Separation of antigen-specific lymphocytes. A new general method of releasing cells bound to nylon mesh.
Inserting a disulfide bridge between nylon and hapten or antigen allows the release of fiber bound cells by cleaving the disulfide bond with 10(-4) M 2-mercaptoethanol at 4 degrees C. Four to 12-fold enrichments in specific precursor cells could be obtained. The method is compared with the thermal release previously used. The T and B cell content of the specific fraction was determined. It is suggested that the low enrichment in plaque-forming cells of the hapten-specific fraction found after stimulation with antigen in vitro might be due to the fact that there is also enrichment for suppressor cells in the specific fraction.
Kiefer
H
H
Basel Institute for Immunology, 487, Grenzacherstrasse, CH-4058 Basel, Switzerland.
D000818:Animals; D000941:Antigens / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D004220:Disulfides; D006241:Haptens; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D009757:Nylons / Q000737:chemistry; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D004220:Disulfides; D006241:Haptens; D009757:Nylons