title: Virosome-mediated delivery of protein antigens to dendritic cells.
abstract: Virosomes are reconstituted viral membranes in which protein can be encapsulated. Fusion-active virosomes, fusion-inactive virosomes and liposomes were used to study the conditions needed for delivery of encapsulated protein antigen ovalbumin (OVA) to dendritic cells (DCs) for MHC class I and II presentation. Fusion-active virosomes, but not fusion-inactive virosomes, were able to deliver OVA to DCs for MHC class I presentation at picomolar OVA concentrations. Fusion activity of virosomes was not required for MHC class II presentation of antigen. Therefore, virosomes are an efficient system for delivery of protein antigens for stimulation of both helper and CTL responses.
authors: Department of Medical Microbiology, Molecular Virology Section, University of Groningen, Groningen, The Netherlands.
authors: Laura
authors: L
authors: Bungener
authors: Karine
authors: K
authors: Serre
authors: Liesbeth
authors: L
authors: Bijl
authors: Lee
authors: L
authors: Leserman
authors: Jan
authors: J
authors: Wilschut
authors: Toos
authors: T
authors: Daemen
authors: Patrick
authors: P
authors: Machy
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D015703:Antigens, CD / Q000096:biosynthesis; D018122:B7-1 Antigen / Q000096:biosynthesis; D051940:B7-2 Antigen; D015415:Biomarkers; D019013:CD40 Antigens / Q000096:biosynthesis; D003593:Cytoplasm; D003713:Dendritic Cells / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D008081:Liposomes; D008561:Membrane Fusion; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010047:Ovalbumin / Q000276:immunology; D017452:Receptors, IgG / Q000276:immunology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D022701:Virosomes
chemical_list: D000941:Antigens; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D015415:Biomarkers; D019013:CD40 Antigens; C497965:Cd86 protein, mouse; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D008081:Liposomes; D008562:Membrane Glycoproteins; D017452:Receptors, IgG; D022701:Virosomes; D018799:Intercellular Adhesion Molecule-1; D010047:Ovalbumin