Anti-inflammatory, analgesic activity and acute toxicity of Glaucium grandiflorum extract.
The species of Glaucium have been used in Iranian herbal medicine as laxative, hypnotic, antidiabetic agents and also in the treatment of dermatitis. The anti-inflammatory and analgesic effects of the aerial parts of Glaucium grandiflorum Boiss & Huet (Papaveraceae), a native plant of Iran, were studied using carrageenan induced edema, formalin and hot plate tests. The G. grandiflorum extract at the dose of 200 mg/kg had more edema inhibition than indomethacin at the doses of 10 (P<0.01) and 8 mg/kg (P<0.001) in the carrageenan test. The ED(50) (i.p.) in the edema induced by carrageenan was 13.59 mg/kg. In formalin test, the extract (60-90 mg/kg, i.p.) caused graded inhibition of both phases of formalin-induced pain. In hot plate test, the i.p. administration of the extract at the doses of 60, 70, 80 and 90 mg/kg significantly raised the pain threshold at a observation time of 45 min in comparison with control (P<0.001). The extract, at the antinociceptive doses, did not affect motor coordination of animals when assessed in the rotarod model. The 72 h acute LD(50) value of this extract after i.p. administration in mice was 797.94 mg/kg.
Morteza-Semnani
Katayoun
K
Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, 48175-861, Iran. semnani_k@yahoo.co.uk
Saeedi
Madjid
M
Hamidian
Mahmood
M
Vafamehr
Hamid
H
Dehpour
Ahmad Reza
AR
D000700:Analgesics / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D008297:Male; D051379:Mice; D010147:Pain Measurement; D028101:Papaveraceae; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D002351:Carrageenan