Effects of waterborne exposure to 4-nonylphenol on plasma sex steroid and vitellogenin concentrations in sexually mature male carp (Cyprinus carpio).
4-Nonylphenol (NP) has been shown to elicit estrogenic responses both in vivo and in vitro. The mechanism by which NP exerts estrogenic and other endocrine-modulating effects in vivo remains unclear, however. The goal of this study was to evaluate the ability of NP to elicit estrogenic responses through indirect mechanisms of action involving the modulation of endogenous steroid hormone concentrations. Sexually mature male common carp (Cyprinus carpio) were exposed to aqueous NP concentrations ranging from <0.05 to 5.4 microg NP/l for 28-31 d. Approximately 0.5-3.5 ppm of NP was detected in pooled plasma samples or tissue samples from the carp studied. NP exposure did not significantly increase plasma concentrations of 17beta-estradiol (E2), testosterone (T) or vitellogenin (VTG). Excluding outliers, plasma E2 concentrations ranged from <175 to 700 pg E2/ml. T concentrations ranged from 940 to 24,700 pg T/ml plasma. The greatest VTG concentration detected was 52 microg/ml. One-third of the plasma samples tested contained <1 microg VTG/ml. Overall, the results of this study did not support the hypothesis that exposure to waterborne NP can modulate concentrations of steroid hormones in the plasma of sexually mature male carp. The results did, however, raise a number of questions regarding the utility of estradiol equivalent (EEQ) estimates as a means of predicting in vivo effects of estrogenic substances. Furthermore, they provide information regarding the concentrations and variability of E2, T, and VTG in the plasma of sexually mature male carp, which may aid in design and interpretation of future studies.
Villeneuve
D L
DL
Department of Zoology, Michigan State University, East Lansing 48824, USA. villene1@msu.edu
Villalobos
S A
SA
Keith
T L
TL
Snyder
E M
EM
Fitzgerald
S D
SD
Giesy
J P
JP
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002347:Carps / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004703:Endocrine System / Q000187:drug effects; D004781:Environmental Exposure; D004958:Estradiol / Q000097:blood*; D008297:Male; D010636:Phenols / Q000009:adverse effects*; D012016:Reference Values; D013739:Testosterone / Q000097:blood*; D014819:Vitellogenins / Q000097:blood* / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
D015415:Biomarkers; D010636:Phenols; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D013739:Testosterone; D004958:Estradiol; C041594:4-nonylphenol