MS3 using the collision cell of a tandem mass spectrometer system.
We report the feasibility of multistage fragmentation in combination with a fast background subtraction method, yielding the equivalent of MS3. The first quadrupole selects an ion of interest, and the ion is axially accelerated into Q2 to generate fragment ions. Subsequent stages of mass selection and fragmentation are obtained by quadrupolar resonant excitation within the Q2 collision cell. The fragments are analyzed downstream by either a resolving quadrupole or a time-of-flight (TOF) mass spectrometer, and multistage spectra are obtained by subtraction (MS(n) - MS(n-1)) for n = 3 or 4. We discuss the characterization of this method, including product ion arrival times, fragmentation efficiencies, and ion selectivity. We report accurate TOF mass spectra of background-subtracted MS3 for protonated molecules reserpine (m/z 609), bosentan (m/z 1552), and taxol (m/z 854).
Cousins
Lisa M
LM
MDS SCIEX, 71 Four Valley Dr., Concord, Ontario, Canada L4K 4V8. LisaC@Ionics.ca
Thomson
Bruce A
BA
D000077300:Bosentan; D005240:Feasibility Studies; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D017239:Paclitaxel / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis; D012110:Reserpine / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods; D013449:Sulfonamides / Q000032:analysis
D004364:Pharmaceutical Preparations; D013449:Sulfonamides; D012110:Reserpine; D017239:Paclitaxel; D000077300:Bosentan