Development of a kinetic basis for bioavailability of sorbed naphthalene in soil slurries.
The degradation of naphthalene in soil-slurry systems was studied using four different organisms and two soils. Organisms with zero-order, first-order, and Michaelis-Menten rates were selected. The soils had substantially different sorption distribution coefficients. Sorption and desorption was evaluated in abiotic soil-slurry systems. The desorption process was described by a model that accounts for equilibrium, rate-limited and non-desorbing sites. Biodegradation parameters were measured in soil-extract solutions. Bioavailability assays, inoculated soil slurries, were conducted and both liquid- and sorbed-phase naphthalene concentrations were measured over time. For the less sorptive soil, the results could be explained by sequential desorption and degradation processes. For the other soil, enhanced degradation was clearly observed for the organisms with first-order and Michaelis-Menten rates. Several explanations are explored for these observations including direct sorbed-phase degradation and the development of elevated substrate concentrations at the organism/sorbent interface. No enhancement was found for the organism with zero-order kinetics.
Park
Jeong-Hun
JH
Department of Civil and Environmental Engineering, Michigan State University, East Lansing 48824, USA.
Zhao
Xianda
X
Voice
Thomas C
TC
D001682:Biological Availability; D002448:Cell Adhesion; D002455:Cell Division; D007700:Kinetics; D008954:Models, Biological; D009281:Naphthalenes / Q000032:analysis / Q000378:metabolism*; D011549:Pseudomonas / Q000166:cytology / Q000378:metabolism*; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
D009281:Naphthalenes; D012987:Soil; D012989:Soil Pollutants; C031721:naphthalene