[Study on the expression of endothelial nitric oxide synthase and its mRNA in liver tissues of patients with chronic hepatitis B].
BACKGROUND
To study the expression of endothelial nitric oxide synthase (eNOS) and its mRNA in liver tissues of patients with chronic hepatitis B (CHB) and the relationship between its expression and pathological changes of hepatic tissues and the clinical significance.


METHODS
48 cases of specimens of liver tissues with CHB were observed and analyzed using immunohistochemical method and in situ nucleic acid hybridization.


RESULTS
eNOS was expressed in hepatocytes, sinusiod endothelial cells and vessel endothelial cells, being predominantly expressed in hepatocytes. The number and distribution of eNOS mRNA positive cells were similiar to those of its protein. Increase in expression of eNOS was correlated with degrees of inflammatory activity and fibrosis (r=0.6855 [P<0.05]; r=0.4828 [P<0.05]; respectively), and with decreasing PA and ALB (F=3.4101 [P<0.05]; F=9.1714 [P<0.0]). Changes of expression of TGF-alpha-R were parallel to those of eNOS.


CONCLUSIONS
eNOS may be involved in pathological lesion of CHB by affecting the expression of TGF-R in liver tissues with CHB.
He
J
J
The 302nd Hospital of PLA, Beijing 100039, China.
Xin
S
S
Zhao
J
J
D000328:Adult; D004727:Endothelium / Q000201:enzymology; D005260:Female; D019694:Hepatitis B, Chronic / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis
D012333:RNA, Messenger; D019001:Nitric Oxide Synthase