Remote sensing of gene expression in Planta: transgenic plants as monitors of exogenous stress perception in extraterrestrial environments.
Transgenic arabidopsis plants containing the alcohol dehydrogenase (Adh) gene promoter fused to the green fluorescent protein (GFP) reporter gene were developed as biological sensors for monitoring physiological responses to unique environments. Plants were monitored in vivo during exposure to hypoxia, high salt, cold, and abcissic acid in experiments designed to characterize the utility and responses of the Adh/GFP biosensors. Plants in the presence of environmental stimuli that induced the Adh promoter responded by expressing GFP, which in turn generated a detectable fluorescent signal. The GFP signal degraded when the inducing stimulus was removed. Digital imaging of the Adh/GFP plants exposed to each of the exogenous stresses demonstrated that the stress-induced gene expression could be followed in real time. The experimental results established the feasibility of using a digital monitoring system for collecting gene expression data in real time from Transgenic Arabidopsis Gene Expression System (TAGES) biosensor plants during space exploration experiments.
Manak
Michael S
MS
Plant Molecular and Cellular Biology, Horticulture Sciences Department, University of Florida, Gainesville, FL 32611-0690, USA.
Paul
Anna-Lisa
AL
Sehnke
Paul C
PC
Ferl
Robert J
RJ
D000426:Alcohol Dehydrogenase / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D015374:Biosensing Techniques; D005118:Extraterrestrial Environment; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D007091:Image Processing, Computer-Assisted; D008164:Luminescent Proteins / Q000235:genetics; D018521:Plant Physiological Phenomena; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000502:physiology; D011401:Promoter Regions, Genetic; D013686:Telemetry
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D000426:Alcohol Dehydrogenase