title: Toxicity of argemone oil: effect on hsp70 expression and tissue damage in transgenic Drosophila melanogaster (hsp70-lacZ) Bg9.
abstract: The effect of argemone oil on hsp70 expression and tissue damage was investigated by studying beta-galactosidase activity, Western blotting and hybridization, and trypan blue staining in the larval tissues of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9. Different concentrations of argemone oil were mixed with food and third-instar larvae were allowed to feed on them for different time intervals (2, 4, 24, and 48 h). Argemone oil was found to induce hsp70 even in the lowest concentration of the adulterant while maximum tissue damage was observed in the higher two treatment groups. Malpighian tubules and midgut tissue reflected maximum damage as evidenced by both high beta-galactosidase activity and trypan blue staining in these tissues. A prior temperature shock treatment to the larvae was enough to protect the larvae from argemone oil-induced tissue damage as evidenced by little or no trypan blue staining. The present study suggests the cytotoxic potential of argemone oil and further strengthens the evidence for the use of hsp70 as a biomarker in risk assessment.
authors: Embryotoxicology Section, Industrial Toxicology Research Centre, Lucknow, India.
authors: I
authors: I
authors: Mukhopadhyay
authors: A
authors: A
authors: Nazir
authors: K
authors: K
authors: Mahmood
authors: D K
authors: DK
authors: Saxena
authors: M
authors: M
authors: Das
authors: S K
authors: SK
authors: Khanna
authors: D K
authors: DK
authors: Chowdhuri
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004330:Drosophila / Q000187:drug effects / Q000196:embryology / Q000235:genetics / Q000502:physiology*; D005502:Food; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D018869:Heat-Shock Response / Q000187:drug effects / Q000502:physiology; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D010938:Plant Oils / Q000633:toxicity*; D014343:Trypan Blue; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D010938:Plant Oils; C030610:argemone oil; D001616:beta-Galactosidase; D014343:Trypan Blue