Genome aberrations observed by restriction landmark genome scanning analysis of chromosomal DNA in various types of primary hepatocellular carcinoma.
OBJECTIVE
The genes responsible for hepatocellular carcinoma have not been identified. To identify the relevant genes of hepatocellular carcinoma, detailed and comprehensive information of genomic aberrations must be obtained. To reveal the chromosomal aberrations in hepatocellular carcinoma, we carried out a restriction landmark genome scanning analysis of various types of hepatocellular carcinoma.


METHODS
Samples of various types of hepatocellular carcinoma, including two with multinodular-hepatocellular carcinomas, one hepatocellular carcinoma showing nodules in a nodule pattern, one hepatocellular carcinoma metastasized to different tissues, three small (< 2.0 cm) hepatocellular carcinomas and four large (> 5.0 cm) hepatocellular carcinomas were examined by the restriction landmark genome scanning method with corresponding non-hepatocellular carcinoma tissues. Restriction enzyme Not I was used as a landmark enzyme, Eco RV was used as a fragmentation enzyme, and Hin fI was used as a digestion enzyme in the gel for two-dimensional electrophoresis.


RESULTS
We observed spot aberrations with different origins. Frequently observed spot aberrations originated from the change in the methylation status of repetitive sequences. No clear correlation between the pathological grade and the number or type of spot aberrations was observed.


CONCLUSIONS
We demonstrated that major aberrations of restriction landmark genome scanning spots originated from the change of methylation status in hepatocellular carcinoma.
Hanafusa
Tadashi
T
Isotope Center, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8558, Japan. hanafusa@cc.okayamau-u.ac.jo
Yumoto
Yasuhiro
Y
Mimura
Tetsushige
T
Shinji
Toshiyuki
T
Hada
Hajime
H
Koide
Norio
N
Tsuji
Takao
T
D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002869:Chromosome Aberrations; D003001:Cloning, Molecular / Q000379:methods; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000032:analysis*; D016147:Genes, Tumor Suppressor; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D004273:DNA, Neoplasm