Biologic monitoring of dental office sterilizers in Mexico.
BACKGROUND
Biologic indicators (BIs) are the quality control applicable to sterilization cycles, but their use was not previously taught in Mexican dental schools or recommended by professional associations. A Mexican official standard, the Mexican Official Norm, published by health authorities in 1999 makes it compulsory for dentists to biologically verify sterilization cycles. However, only a few dentists comply because the use of BIs is largely unknown and the standard is not being enforced.


OBJECTIVE
To evaluate the incidence of sterilization failures in a convenience sample of dental offices in Mexico.


METHODS
Spore strips were distributed to dentists interested in using this service. The dentists mailed the processed BIs to the laboratory for culture.


RESULTS
In 6 years, 3277 tests were submitted from 82 dental offices. Thirty-four offices (41%) submitted 1 to 12 tests, 22 (27%) sent 14 to 48 tests, 18 (22%) mailed 49 to 96 tests, and 8 (10%) sent >97 tests. The sterilization methods were steam (74.4%), dry heat (20%), and chemical vapor (5.6%). A total of 242 sterilization failures (7.4% of all cycles) were detected. Convection dry heat failed with a greater frequency with chi(2) analysis (chi(2) = 13.71, P =.0175).


CONCLUSIONS
Sterilization failures occurred in instrument loads used in patient treatment. Steam and chemical vapor under pressure failed less often than convection dry heat. When corrective action is taken, routine use of BIs increases patient safety.
Acosta-Gío
A Enrique
AE
Microbiology and Infection Control Laboratory, Postgraduate Division, National University of Mexico Dental School, Mexico City, Mexico.
Mata-Portuguez
Víctor Hugo
VH
Herrero-Farías
Aurelio
A
Sánchez Pérez
Leonor
L
D016009:Chi-Square Distribution; D003748:Dental Equipment / Q000592:standards; D003770:Dental Offices; D003815:Dentists / Q000592:standards*; D004784:Environmental Monitoring; D004866:Equipment Contamination / Q000706:statistics & numerical data*; D006801:Humans; D007202:Indicators and Reagents; D017053:Infection Control / Q000379:methods / Q000592:standards / Q000706:statistics & numerical data; D008800:Mexico; D011786:Quality Control; D013242:Sterilization / Q000295:instrumentation* / Q000379:methods / Q000592:standards*
D007202:Indicators and Reagents