title: Cell kinetics of slow renewing cell populations in mice stomach.
abstract: BACKGROUND
  The renewal rates of parietal and chief cells in the gastric mucosa and smooth muscle cells of muscularis propria have not been examined as precisely as superficial epithelial cells. To examine cell renewal of these cells, continuous labeling with tritiated ([3H])-thymidine was performed.
  
  
  METHODS
  Mice received 112 repeated injections of [3H]-thymidine at 6-hour intervals for 28 days after birth and were killed immediately thereafter, or 60, 120, 200 or 300 days after the last injection.
  
  
  RESULTS
  After continuous labeling, most cells in the stomach were labeled. At 60 days, unlabeled parietal cells in the neck area of the gland and unlabeled chief cells in the middle part of the gland appeared. Thereafter, the area of unlabeled cells expanded downwards to the bottom of the gland. Times required for labeling of total cell populations of parietal and chief cells to half were less than 60 days and more than 200 days, respectively. At 300 days, most parietal cells and about half of the chief cells remained labeled in the bottom of the gland. The labeling index of smooth muscle cells was about 100% for 300 days.
  
  
  CONCLUSIONS
  The time required for the newly formed parietal and chief cells to reach the lower end of the gland was more than 300 days. As a total cell population, the renewal rate of parietal cells was more rapid than that of chief cells. However, in terms of the downward migrating cell population, the renewal rate of parietal cells was a little slower than that of chief cells. Smooth muscle cells showed almost no renewal.
authors: Division of Gene Therapy, Intractable Disease Research Center, Tokyo Medical University, Tokyo, Japan. magami@tokyo-med.ac.jp
authors: Yasushi
authors: Y
authors: Magami
authors: Takeshi
authors: T
authors: Azuma
authors: Hideto
authors: H
authors: Inokuchi
authors: Fuminori
authors: F
authors: Moriyasu
authors: Keiichi
authors: K
authors: Kawai
authors: Takanori
authors: T
authors: Hattori
mesh_terms: D000818:Animals; D001345:Autoradiography; D002453:Cell Cycle; D002465:Cell Movement; D019872:Chief Cells, Gastric / Q000166:cytology*; D005753:Gastric Mucosa / Q000166:cytology; D008297:Male; D051379:Mice; D009130:Muscle, Smooth / Q000166:cytology; D010295:Parietal Cells, Gastric / Q000166:cytology*; D013936:Thymidine; D013997:Time Factors; D014316:Tritium
chemical_list: D014316:Tritium; D013936:Thymidine