Intracellular insulin-responsive glucose transporter (GLUT4) distribution but not insulin-stimulated GLUT4 exocytosis and recycling are microtubule dependent.
To investigate the potential role of microtubules in the regulation of insulin-responsive glucose transporter (GLUT4) trafficking in adipocytes, we examined the effects of microtubule depolymerizing and stabilizing agents. In contrast to previous reports, disruption or stabilization of microtubule structures had no significant effect on insulin-stimulated GLUT4 translocation. However, consistent with a more recent study (Molero, J. C., J. P. Whitehead, T. Meerloo, and D. E. James, 2001, J Biol Chem 276:43829-43835) nocodazole did inhibit glucose uptake through a direct interaction with the transporter itself independent of the translocation process. In addition, the initial rate of GLUT4 endocytosis was not significantly affected by microtubule depolymerization. However, these internalized GLUT4 compartments are confined to regions just beneath the plasma membrane and were not exposed to the extracellular space. Furthermore, they were unable to undergo further sorting steps and trafficking to the perinuclear region. Nevertheless, these apparent early endocytic GLUT4 compartments fully responded to a second insulin stimulation with an identical extent of plasma membrane translocation. Together, these data demonstrate that although microtubular organization may play a role in the trafficking of GLUT4 early endocytic vesicles back to the perinuclear region, they do not have a significant role in insulin-stimulated GLUT4 exocytosis, initial endocytosis from the plasma membrane and/or recycling back to the plasma membrane.
Shigematsu
Satoshi
S
Department of Physiology & Biophysics, The University of Iowa, Iowa City, Iowa 52242, USA.
Khan
Ahmir H
AH
Kanzaki
Makoto
M
Pessin
Jeffrey E
JE
D016475:3T3 Cells; D017667:Adipocytes / Q000378:metabolism / Q000648:ultrastructure*; D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D004705:Endocytosis; D005089:Exocytosis; D005455:Fluorescent Antibody Technique; D005947:Glucose / Q000378:metabolism; D051275:Glucose Transporter Type 4; D049452:Green Fluorescent Proteins; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008870:Microtubules / Q000502:physiology*; D009004:Monosaccharide Transport Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins; D015739:Nocodazole / Q000494:pharmacology; D011108:Polymers / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D011993:Recombinant Fusion Proteins
D051275:Glucose Transporter Type 4; D007328:Insulin; D008164:Luminescent Proteins; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D011108:Polymers; D016271:Proto-Oncogene Proteins c-myc; D011993:Recombinant Fusion Proteins; C495693:Slc2a4 protein, mouse; D049452:Green Fluorescent Proteins; D005947:Glucose; D015739:Nocodazole