Monoclonal antibody-based enzyme immunoassay for detection of ricin.
A sensitive and specific enzyme-linked immunoadsorbent assay (ELISA) was developed to detect ricin in biological fluids. The assay is based on the sandwich format using monoclonal antibodies (MAbs) of two distinct specificities. An affinity-purified anti-ricin B chain MAb (1G7) is utilized to adsorb ricin from solution and the second anti-ricin A chain MAb (5E11) conjugated with peroxidase is then used to form a sandwich, and peroxidase allows color development and measurement of optical density at 450 nm. Standard curves were linear over the range of 2.5-100 ng/mL ricin. The limit of detection was below 5 ng/mL in assay buffer as well as in a 1:10 dilution of urine or 1:50 dilution of human serum spiked with ricin.
Shyu
Huey-Fen
HF
Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Chiao
Der-Jiang
DJ
Liu
Hwan-Wun
HW
Tang
Shiao-Shek
SS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D015153:Blotting, Western; D002624:Chemistry, Clinical / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D016014:Linear Models; D051379:Mice; D008807:Mice, Inbred BALB C; D009195:Peroxidase / Q000378:metabolism; D012276:Ricin / Q000032:analysis* / Q000097:blood / Q000652:urine; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D012276:Ricin; D009195:Peroxidase