Expression of proenkephalin (PENK) mRNA in inflammatory leukocytes during experimental peritonitis in Swiss mice.
Zymosan- or thioglycollate-induced experimental peritoneal inflammation in mice may serve as a convenient model for investigations of involvement of opioid peptides derived from exudatory leukocytes in the inflammatory processes. During peritonitis, the influx of neutrophils and monocytes/macrophages correlated with a sequential appearance of proinflammatory cytokines (IL-1beta and TNFalpha). After both kinds of stimulation, the expression of PENK mRNA was much higher in exudatory peritoneal leukocytes than its basal level in steady state.
Chadzinska
M
M
Department of Evolutionary Immunobiology, Institute of Zoology, Jagiellonian University, Kraków, Poland.
Maj
M
M
Scislowska-Czarnecka
A
A
Przewłocka
B
B
Plytycz
B
B
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D004745:Enkephalins / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007375:Interleukin-1 / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D010538:Peritonitis / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013864:Thioglycolates; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015054:Zymosan
D004745:Enkephalins; D007375:Interleukin-1; D011498:Protein Precursors; D012333:RNA, Messenger; D013864:Thioglycolates; D014409:Tumor Necrosis Factor-alpha; C029992:proenkephalin; C017487:2-mercaptoacetate; D015054:Zymosan