[Gene expression pattern of senescence associated genes in rat muscle].
This work is aimed at the investigation of the senescence associated genes differentially expressed in rat muscle by cDNA microarray. After total RNA isolation, mRNA purification and probe preparation, the gene expression patterns of rat muscle from the old group (30 months old) and the young group (3 months old) were compared by cDNA gene chips. In the muscle of old rats there were reproducibly 127 differentially expressed genes, among which some down-regulated genes were involved in energy metabolism and signal transduction, while some up-regulated genes were related to protein degradation and cell apoptosis. Meanwhile muscle fiber atrophy with mitochondria vacuolation was found in aging rat by electron microscope.
Zhang
Fang-Lin
FL
Shanghai Second Medical University, Ruijin Hospital, Shanghai Institute of Endocrinology, Shanghai 200025, China.
Huang
Guo-Liang
GL
Ye
Chuan-Zhong
CZ
Zhang
Kai-Zhen
KZ
Lin
Zhe-Zhang
ZZ
Chen
Xiao-Chun
XC
You
Jie
J
D000375:Aging; D000818:Animals; D020869:Gene Expression Profiling; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger