title: Cholinesterase activity and acetylcholinesterase glycosylation are altered in human breast cancer.
abstract: Increasing evidence supports the involvement of cholinesterases in tumorigenesis. Several tumour cells show ChE activity, while the acetyl- (AChE) and butyrylcholinesterase (BuChE) genes are amplified in leukemias, ovarian carcinoma and other cancers. ChE activity was measured in 31 samples of tumoral breast (TB) and 20 of normal breast (NB). Despite the wide variations observed, BuChE predominated over AChE both in TB and NB. The mean AChE activity in NB was 1.61 nmol of the substrate hydrolysed per minute and per miligram protein (mU/mg), which rose to 3.09 mU/mg in TB (p = 0.041). The BuChE activity dropped from 5.24 mU/mg in NB to 3.39 mU/mg in TB (p = 0.002). Glycolipid-linked AChE dimers and monomers and hydrophilic BuChE tetramers and monomers were identified in NB and TB, and their proportions were unmodified by the neoplasia. The amount of AChE forms reacting with wheat germ agglutinin (WGA) decreased in TB while that of BuChE species was unaffected, demonstrating that the glycosylation of AChE was altered in TB. The binding of AChE and BuChE with antibodies was unaffected by the neoplasia. The difference in lectin reactivity between erythrocyte and breast AChE, the lack of AChE in blood plasma, and the finding of monomeric BuChE in breast but not in plasma suggest that breast epithelial cells produce AChE for membrane attachment and hydrophilic BuChE for secretion. Several reasons are provided to explain the altered expression of ChEs in breast cancer.
authors: Departamento de Bioquímica y Biología Molecular-A, Edificio de Veterinaria, Universidad de Murcia, Spain.
authors: Francisco
authors: F
authors: Ruiz-Espejo
authors: Juan
authors: J
authors: Cabezas-Herrera
authors: Julián
authors: J
authors: Illana
authors: Francisco J
authors: FJ
authors: Campoy
authors: Cecilio J
authors: CJ
authors: Vidal
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001940:Breast / Q000201:enzymology*; D001943:Breast Neoplasms / Q000201:enzymology*; D002091:Butyrylcholinesterase / Q000378:metabolism; D016022:Case-Control Studies; D002802:Cholinesterases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006031:Glycosylation; D006801:Humans; D008875:Middle Aged
chemical_list: D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases