title: The OhrR repressor senses organic hydroperoxides by reversible formation of a cysteine-sulfenic acid derivative.
abstract: Reactive oxygen species induce the expression of detoxification and repair genes critical for life in an aerobic environment. Bacterial factors that sense reactive oxygen species use either thiol-disulfide exchange reactions (OxyR, RsrA) or redox labile 2Fe-2S clusters (SoxR). We demonstrate that the reduced form of Bacillus subtilis OhrR binds cooperatively to two adjacent inverted repeat sequences in the ohrA control region and thereby represses transcription. In the presence of organic hydroperoxides, OhrR is inactivated by the reversible oxidation of a single conserved cysteine residue to the corresponding cysteine-sulfenic acid, and perhaps to higher oxidation states.
authors: Department of Microbiology, Cornell University, Ithaca, NY 14853-8101,USA.
authors: Mayuree
authors: M
authors: Fuangthong
authors: John D
authors: JD
authors: Helmann
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001555:Benzene Derivatives / Q000378:metabolism* / Q000494:pharmacology; D003545:Cysteine / Q000378:metabolism*; D004269:DNA, Bacterial; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D016877:Oxidants / Q000378:metabolism* / Q000494:pharmacology; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013434:Sulfenic Acids / Q000378:metabolism*; D014157:Transcription Factors; D020122:tert-Butylhydroperoxide / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D001555:Benzene Derivatives; D004269:DNA, Bacterial; D016877:Oxidants; D012097:Repressor Proteins; D013434:Sulfenic Acids; D014157:Transcription Factors; C122138:peroxide repressor proteins; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D003545:Cysteine; C007164:cumene hydroperoxide