title: Different proteins are phosphorylated under acidic environments in Jurkat cells.
abstract: In order to test the ability of immune cells to grow under acidic conditions, Jurkat cells were cultured in medium at pH 6.3. At this acidic pH, the cells required approximately 2 days per generation, while the generation time was approximately 1 day for cells grown in near neutral medium. Western blot analysis using anti-phosphotyrosine antibody (4G10) showed that protein phosphorylation was affected by external pH. For example, the level of a 66-kDa phosphoprotein increased dramatically in acidic medium, whereas the amount of a 34-kDa phosphoprotein decreased. These results suggest that Jurkat cells use different signal pathways under acidic environments.
authors: Graduate School of Pharmaceutical Sciences, Chiba University, 1-33, Yayoi-cho, Inage-ku, Chiba, Japan.
authors: Toshihiko
authors: T
authors: Fukamachi
authors: Hiromi
authors: H
authors: Saito
authors: Tomohito
authors: T
authors: Kakegawa
authors: Hiroshi
authors: H
authors: Kobayashi
mesh_terms: D002455:Cell Division; D003600:Cytosol / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019169:Jurkat Cells; D007700:Kinetics; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D010750:Phosphoproteins