Effect of cationic liposomes in an in vitro transcription and translation system.
The effects of cationic liposomes complexed with plasmid DNA on the process of transcription was examined using a recently developed rapid cell free translation system. The findings indicate that the liposome itself inhibited the process when the ratio of DNA/liposome typically used in transfection studies was used.
Tachibana
Rieko
R
Faculty of Pharmaceutical Sciences, The University of Tokushima, Japan.
Harashima
Hideyoshi
H
Ishida
Tatsuhiro
T
Shinohara
Yasuo
Y
Hino
Mami
M
Terada
Hiroshi
H
Baba
Yoshinobu
Y
Kiwada
Hiroshi
H
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002412:Cations; D008081:Liposomes / Q000494:pharmacology*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis / Q000187:drug effects*; D011506:Proteins / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*
D002412:Cations; D008081:Liposomes; D011506:Proteins