title: Distribution of the long leptin receptor isoform in brush border, basolateral membrane, and cytoplasm of enterocytes.
abstract: OBJECTIVE
  Leptin, a hormone mainly produced by fat cells, acts primarily on the hypothalamus regulating energy expenditure and food intake. Leptin receptors are expressed in several tissues and the possible physiological role of leptin is being extensively investigated, with the result that important peripheral actions of the hormone in the organism are being discovered. Recent studies have demonstrated leptin and leptin receptor expression in gastric epithelial cells. In the present study, we report the presence of the long leptin receptor isoform (OB-Rb) in human, rat, and mouse small intestine, supporting the hypothesis of leptin as a hormone involved in gastrointestinal function.
  
  
  METHODS
  The presence of the leptin receptor was determined by immunocytochemical methods using antibodies against the peptide corresponding to the carboxy terminus of the long isoform of the leptin receptor. Human duodenal biopsies from normal individuals undergoing gastrointestinal endoscopy, and intestinal fragments of Wistar rats and Swiss mice were processed for the study.
  
  
  RESULTS
  Immunoreactivity for the long leptin receptor isoform was observed in the three studied species. Staining was located throughout the cytoplasm of the enterocytes, of both villi and crypts, and in the basolateral plasma membrane. Immunolabelling for OB-Rb protein was also found in the brush border of human enterocytes of formol and paraformaldehyde fixed samples.
  
  
  CONCLUSIONS
  This report demonstrates the presence of the long leptin receptor isoform in the absorptive cells of rat, mouse, and human small intestine, suggesting that leptin could have a physiological role in the regulation of nutrient absorption.
authors: Department of Physiology and Nutrition, University of Navarra, Pamplona, Spain.
authors: J
authors: J
authors: Barrenetxe
authors: A C
authors: AC
authors: Villaro
authors: L
authors: L
authors: Guembe
authors: I
authors: I
authors: Pascual
authors: M
authors: M
authors: Muñoz-Navas
authors: A
authors: A
authors: Barber
authors: M P
authors: MP
authors: Lostao
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004386:Duodenum / Q000378:metabolism*; D020895:Enterocytes / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007583:Jejunum / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002352:Carrier Proteins; C515448:LEPR protein, human; D020738:Leptin; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; C515447:leptin receptor, mouse