In vitro study of urease/AlaDH enzyme system encapsulated into human erythrocytes and research into its medical applications.
In our system, urease/AlaDH have been encapsulated within erythrocytes by using slow dialysis methods. Urea is decomposed into ammonia and bicarbonate and the ammonia released is converted into alanine by reacting pyruvate under the catalytic action of AlaDH. It is very important for our that products are formed quickly but the ammonia is not connected definetely. For this aim, urease/AlaDH we encapsulated using different enzyme activity ratio (0.5:1.5; 0.5:2.5; 0.25:1.25 U/U urease/AlaDH). The activities of enzyme systems, encapsulation yield, McV, McH, and McHc were measured for each sample. Investigated results suggest that loaded enzyme systems can be used as potential carrier systems for the removal of high levels of urea from blood.
Hamarat Baysal
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Faculty of Science Biochemistry Department, Ege University, Izmir, Turkey. hamarat@sci.ege.edu.tr
Uslan
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AH
D050788:Alanine Dehydrogenase; D000594:Amino Acid Oxidoreductases / Q000378:metabolism / Q000494:pharmacology*; D000641:Ammonia / Q000378:metabolism; D002214:Capsules; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D019289:Pyruvic Acid / Q000378:metabolism; D014508:Urea / Q000378:metabolism*; D014510:Urease / Q000378:metabolism / Q000494:pharmacology*
D002214:Capsules; D000641:Ammonia; D019289:Pyruvic Acid; D014508:Urea; D000594:Amino Acid Oxidoreductases; D050788:Alanine Dehydrogenase; D014510:Urease