title: Effects of varying gonadotrophin dose and timing on antrum formation and ovulation efficiency of mouse follicles in vitro.
abstract: BACKGROUND
  This study tested factors affecting mouse follicle growth in vitro, to determine end-points marking follicle function in vitro.
  
  
  METHODS
  Pre-antral follicles (mean 137 microm) from B6CBF1 mice were cultured in a substrate-adherent system for < or = 14 days. FSH (0-1000 mIU/ml) day of HCG (1.5 IU/ml days 9-14) protein supplement [fetal calf serum (FCS) (x2) mouse serum (x2) hypogonadal (hpg) mouse serum or human serum albumin (HSA)] were varied. Follicle survival timing of antrum formation incidence of ovulation within 16,24,40,48 h of HCG oocyte growth were assessed.
  
  
  RESULTS
  FSH (100 mIU/ml) produced the best antral development (P < 0.001 versus 10 and 1000 mIU/ml). Antra were observed from day 5. Transient antra formed occasionally in the absence of FSH. By 14 days significant senescence had occurred (P < 0.001) but the proportion of follicles ovulating within 16 h of HCG declined from day 9 onwards indicating this to be a more sensitive marker of follicle responsiveness. Optimal growth occurred in 5% FCS (x2) or hpg mouse serum although fewer follicles ovulated in hpg serum (P < 0.05). No normal growth occurred in normal mouse serum (x2) or HSA. Oocytes grew to full size within 9 days with 100 mIU/ml FSH FCS.
  
  
  CONCLUSIONS
  These data provide sensitive end-points for assessing follicle growth in vitro.
authors: Department of Biological Sciences University of Warwick Coventry CV4 7AL UK.
authors: Leila M
authors: LM
authors: Mitchell
authors: C Richard
authors: CR
authors: Kennedy
authors: Geraldine M
authors: GM
authors: Hartshorne
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D048429:Cell Size / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000166:cytology; D009994:Osmolar Concentration; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology*; D010060:Ovulation / Q000187:drug effects*; D012709:Serum Albumin / Q000494:pharmacology
chemical_list: D006063:Chorionic Gonadotropin; D012709:Serum Albumin; D005640:Follicle Stimulating Hormone