Potential application of nonstructural protein NS1 serotype-specific immunoglobulin G enzyme-linked immunosorbent assay in the seroepidemiologic study of dengue virus infection: correlation of results with those of the plaque reduction neutralization test.
An NS1 serotype-specific indirect enzyme-linked immunosorbent assay (ELISA) was developed to differentiate primary and secondary dengue virus infections and serotypes of primary dengue virus infection. For this report, we carried out retrospective seroepidemiologic studies on serum samples collected from residents of Liuchiu Hsiang, Pingtung County, an isolated island in southern Taiwan during 1997-1998. The results demonstrated that good correlation existed between dengue virus NS1 serotype-specific immunoglobulin G (IgG) ELISA and dengue virus plaque reduction neutralization test (PRNT). Our data suggested that NS1 serotype-specific IgG ELISA could replace PRNT for seroepidemiologic studies to differentiate Japanese encephalitis and dengue virus infections and for dengue virus serotyping.
Shu
Pei-Yun
PY
Division of Research and Diagnosis, Center for Disease Control, Department of Health, 161 Kun-Wang Street, Taipei, Taiwan, Republic of China.
Chen
Li-Kuang
LK
Chang
Shu-Fen
SF
Yueh
Yi-Yun
YY
Chow
Ling
L
Chien
Li-Jung
LJ
Chin
Chuan
C
Yang
Hui-Hua
HH
Lin
Ting-Hsiang
TH
Huang
Jyh-Hsiung
JH
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D002648:Child; D003715:Dengue / Q000175:diagnosis / Q000453:epidemiology* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006723:Hong Kong / Q000453:epidemiology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008875:Middle Aged; D015995:Prevalence; D012680:Sensitivity and Specificity; D017361:Viral Nonstructural Proteins / Q000302:isolation & purification*; D010948:Viral Plaque Assay
D007074:Immunoglobulin G; C082515:NS1 protein, dengue-1 virus; D017361:Viral Nonstructural Proteins