Cross-reactivity patterns of IgM and IgG anti-lipopolysaccharide antibodies in individual mice.
A sensitive method has been developed which permits comparative analysis of IgM and IgG antibody specificity against lipopolysaccharide (LPS) antigen. It is based on hemolysis of LPS-coated red blood cells and on its inhibition by homologous and heterologous LPS. By appropriate use of anti-immunoglobulin sera, indirect (facilitated) lysis due to IgG antibodies is obtained, whereas IgM gives direct lysis and is 2-mercaptoethanol-sensitive. IgG can be analyzed either by facilitation with a rabbit anti-mouse Ig or with anti-allotype sera. By use of anti-allotype sera in F1 hybrids, both parental antibody types can be studied separately. Antibodies of either class from individual mice may display different cross-reactivity patterns. Furthermore, for IgM and IgG within a given serum, both similarities and differences have been found. Some of the cross-reactivity patterns have been followed over one year. With few exceptions, individual patterns remained constant throughout this period.
Di Pauli
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Immunology Unit, Biology Division, University of Konstanz, D-775 Konstanz, Fed. Rep. Germany.
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000918:Antibody Specificity; D003429:Cross Reactions; D004912:Erythrocytes / Q000276:immunology; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D011817:Rabbits; D012475:Salmonella / Q000276:immunology; D012756:Sheep
D000907:Antibodies, Bacterial; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides