title: Characterization of monoclonal antibodies against the second-generation schizonts of Leucocytozoon caulleryi.
abstract: Monoclonal antibodies (MAbs), R1 and M5, were established against the second-generation schizont of Leucocytozoon caulleryi (L. caulleryi). Both antibodies reacted to membrane and internal structure proteins of the second-generation schizont by immunofluorescence microscopy. Molecular weight of the second-generation schizont (2GS) antigen was analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. At least 40 protein bands were detected in 2GS antigen by SDS-PAGE under reduced condition and ranged from 10 to 270 kDa. MAb R1 reacted to polypeptides of 150-268 kDa in 2GS antigen, whereas MAb M5 did with that of 66 kDa. Injection with a protein of 2GS antigen fractionated by affinity chromatography using MAbs R1 and M5 protected chickens against challenge with sporozoites of L. caulleryi. These results suggest that MAbs, R1 and M5, recognize 2GS antigen of L caulleryi.
authors: Research Center for Veterinary Science, The Kitasato Institute, Kitamoto, Saitama, Japan.
authors: Tohru
authors: T
authors: Gotanda
authors: Makito
authors: M
authors: Doi
authors: Yoshio
authors: Y
authors: Eiguchi
authors: Yoshio
authors: Y
authors: Tanaka
authors: Shigeki
authors: S
authors: Kobayashi
authors: Yujirou
authors: Y
authors: Fujisaki
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D002645:Chickens / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D016787:Haemosporida / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence / Q000662:veterinary; D008970:Molecular Weight; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology*; D013047:Specific Pathogen-Free Organisms
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan