Simultaneous determination of the carboxylate and lactone forms of 10-hydroxycamptothecin in human serum by restricted-access media high-performance liquid chromatography.
A simple restricted-access media (RAM) HPLC method for simultaneous determination of the lactone and carboxylate forms of 10-hydroxycamptothecin (HCPT) in human serum was established. Using a RAM Hisep analytical column, serum samples were directly injected into the HPLC system. The eluted peaks of two forms of HCPT were monitored with a fluorescence detector. The separation was completed in 17 min. The linear range was 20-1000 ng/ml, intra-day and inter-day variations being less than 5%. The kinetic equation was introduced according to the analytical results. The equation shows that the course of the HCPT lactone form converting to carboxylate form in human serum at 4 degrees C is a first-order kinetic course. The concentration of each form at the moment of sampling was calculated by extrapolation.
Ma
Jun
J
Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Liu
Chun-Li
CL
Zhu
Peng-Ling
PL
Jia
Zheng-Ping
ZP
Xu
Li-Ting
LT
Wang
Rong
R
D000970:Antineoplastic Agents / Q000097:blood* / Q000737:chemistry; D002138:Calibration; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000097:blood* / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007783:Lactones / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000970:Antineoplastic Agents; D002264:Carboxylic Acids; D007783:Lactones; C028098:10-hydroxycamptothecin; D002166:Camptothecin