Coding single nucleotide polymorphism in the high-affinity immunoglobulin E receptor b chain (FcepsilonRI-beta) gene is associated with immunoglobulin E receptor-mediated histamine release from basophils.
BACKGROUND
Our previous work on linkage analysis showed that histamine release from basophils to anti-IgE stimuli was linked to the gene marker of chromosome 11q13, where the beta chain of the high-affinity receptor for IgE (FcepsilonRI-beta) is located.


OBJECTIVE
To evaluate the association between FcepsilonRI-mediated histamine release from basophils and four bi-allelic single nucleotide polymorphisms of the FcepsilonRI-beta gene.


METHODS
Phenotypes of asthma, such as maximal histamine release from basophils and atopy, were measured from 80 randomly recruited asthmatic children. Polymorphisms of the FcepsilonRI-beta gene were determined by PCR-based methods.


RESULTS
The polymorphism in exon 7, resulting in Glu to Gly substitution, was significantly associated with histamine release from basophils to anti-IgE stimuli, but not with total IgE levels and skin test responses to aeroallergens.


CONCLUSIONS
This study supports a role for the FcepsilonRI-beta gene in the expression of high affinity IgE receptor-mediated histamine release from basophils.
Kim
Y-K
YK
Department of Internal Medicine, Seoul National University College of Medicine, Korea. youyoung@plaza.snu.ac.kr
Oh
S-Y
SY
Oh
H-B
HB
Chun
S-Y
SY
Cho
S-H
SH
Koh
Y-Y
YY
Min
K-U
KU
Kim
Y-Y
YY
D000293:Adolescent; D000483:Alleles; D001249:Asthma / Q000235:genetics*; D001491:Basophils / Q000276:immunology* / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002648:Child; D005260:Female; D006636:Histamine Release / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011897:Random Allocation; D017455:Receptors, IgE / Q000235:genetics* / Q000502:physiology*
D017455:Receptors, IgE; D000001:Calcimycin