Regulation of autolysins in teichuronic acid-containing Bacillus subtilis cells.
Bacillus subtilis cells grown under phosphate starvation induce teichuronic acid (TUA) synthesis while simultaneously repressing teichoic acid synthesis (TA). The turnover rates of TA-containing and TUA-containing walls are similar, indicating that autolysin function is similar and suggesting that modulation of autolytic function may be similar. In this study, it is demonstrated, utilizing fluorescein isothiocyanate (FITC)-dextran to probe the wall pH, that a low pH exists in the wall matrix. A second probe, cationized ferritin (CF), was used to observe cell surface protonation. Suspensions of B. subtilis cells containing either TA or TUA were aggregated with CF only after the addition of a proton-motive-force-dissipating agent. Respiring B. subtilis TUA-containing cells labelled with FITC-dextran exhibited little fluorescence. Conversely, fluorescence intensities exhibited by cells de-energized with nitrogen gas were significantly greater. The effects of protonmotive force on autolytic activity were studied by adding cell wall protein extract containing concentrated autolysin to exponentially growing TA-containing and TUA-containing B. subtilis cells. Both TUA-containing and TA-containing cells were lysed only after the addition of sodium azide. These data suggest that during normal growth the wall of TUA-containing B. subtilis cells is protonated, and proton-motive force influences autolytic regulation in both TUA-containing and TA-containing B. subtilis cells.
Calamita
Heather G
HG
Department of Microbiology and Immunology, University of Louisville Health Sciences Center, KY 40292, USA.
Doyle
R J
RJ
D001412:Bacillus subtilis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D005293:Ferritins / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000378:metabolism*; D009584:Nitrogen / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D018892:Proton-Motive Force / Q000187:drug effects / Q000502:physiology*; D019810:Sodium Azide / Q000494:pharmacology; D013682:Teichoic Acids / Q000378:metabolism; D014574:Uronic Acids / Q000378:metabolism*
D001426:Bacterial Proteins; D010710:Phosphates; D013682:Teichoic Acids; D014574:Uronic Acids; C010788:teichuronic acid; D005293:Ferritins; D019810:Sodium Azide; D009238:N-Acetylmuramoyl-L-alanine Amidase; D009584:Nitrogen