Sticky caveats in an otherwise glowing report: oligomerizing fluorescent proteins and their use in cell biology.
Fluorescent proteins from sea creatures have revolutionized the study of cell biology and signal transduction in many ways. Zacharias discusses some of the technical caveats to working with these proteins when they are fused to cellular proteins to track protein localization and interactions. Special attention is paid to problems arising from oligomerization of these fluorescent proteins and how that impacts protein interactions detected by fluorescence resonance energy transfer (FRET).
Zacharias
David A
DA
Merck Research Laboratories, 3535 General Atomics Court, MRLSDB1 Room 1400, San Diego, CA 92122, USA. David_Zacharias@merck.com
D000818:Animals; D002468:Cell Physiological Phenomena / Q000187:drug effects; D019281:Dimerization; D008164:Luminescent Proteins / Q000009:adverse effects / Q000378:metabolism*; D009842:Oligopeptides / Q000009:adverse effects / Q000378:metabolism*; D011108:Polymers / Q000009:adverse effects / Q000378:metabolism
D008164:Luminescent Proteins; D009842:Oligopeptides; D011108:Polymers