Novel near-infrared cyanine fluorochromes: synthesis, properties, and bioconjugation.
Recently, near-infrared (NIR) fluorescence light has been applied to image various biological events in vivo, because it penetrates tissue more efficiently than light in the visible spectrum. Compounds exhibiting fluorescent properties in the NIR range are key elements for this upcoming optical imaging technology. In this paper, we report the synthesis of four new, water-soluble NIR cyanine fluorochromes which have superior chemical stability and optical properties. Each fluorochrome was designed with a monoreactive carboxyl group for labeling purposes. When multiple fluorochromes were attached to a single macromolecule, fluorescence quenching was observed. On the basis of this property, a novel autoquenched enzyme sensitive NIR fluorescence probe was prepared.
Lin
Yuhui
Y
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown 02129, USA.
Weissleder
Ralph
R
Tung
Ching-Hsuan
CH
D000577:Amides / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002232:Carbocyanines / Q000138:chemical synthesis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D013386:Succinates; D014357:Trypsin / Q000378:metabolism
D000577:Amides; D002232:Carbocyanines; D005456:Fluorescent Dyes; D013386:Succinates; D014357:Trypsin; C011116:succinamide