title: Bacterial adhesion onto azidated poly(vinyl chloride) surfaces.
abstract: A plasticized poly(vinyl chloride) surface was modified by azidation using sodium azide in the presence of a phase transfer catalyst in aqueous media. Subsequent to azidation, the surface was crosslinked using ultraviolet radiation. Contact angle measurements showed that the surface became hydrophilic on azidation whereas photoirradiation did not have any further effect on the hydrophilicity of the azidated surface. Control, azidated, and photocrosslinked surfaces were exposed to two strains of bacteria commonly implicated in device infection such as Staphylococcus aureus and Escherichia coli. Whereas the control and photocrosslinked surfaces showed no significant difference in bacterial adhesion, the azidated surface showed significantly reduced adhesion to both strains. Data obtained indicate that the presence of an intact azide function on the polymer surface is responsible for the reduced bacterial adherence and the surface hydrophobicity/hydrophilicity did not exert any effect in the present case. Although azides are known to be effective only against Gram-negative species, surprising was the observation that the azidated polymer surface was equally effective against a Gram-positive species such as S. aureus. Because sodium azide is routinely used as a preservative to prevent bacterial and fungal growth in many microbiology reagents and diagnostic kits, covalent binding of the azide onto a polymer surface or synthesizing azide containing polymers may be an interesting method to investigate in tackling the problem of bacterial adhesion and colonization of medical devices.
authors: Division of Polymer Chemistry, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Satelmond Palace Campus, Trivandrum 695 012, India.
authors: S
authors: S
authors: Lakshmi
authors: S S Pradeep
authors: SS
authors: Kumar
authors: A
authors: A
authors: Jayakrishnan
mesh_terms: D001386:Azides; D001422:Bacterial Adhesion; D003432:Cross-Linking Reagents / Q000737:chemistry; D004926:Escherichia coli / Q000502:physiology* / Q000648:ultrastructure; D008027:Light; D011143:Polyvinyl Chloride; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000502:physiology* / Q000648:ultrastructure; D013499:Surface Properties
chemical_list: D001386:Azides; D003432:Cross-Linking Reagents; D011143:Polyvinyl Chloride