title: Monitoring of azo dye degradation processes in a bioreactor by on-line high-performance liquid chromatography.
abstract: A technical solution and development of a method for on-line HPLC monitoring of bioreactor processes in a membrane reactor system are presented. Experiences in system design for the continuous coupling of a bioreactor system with capillary by-pass circuits using membrane flow cells and a dual HPLC system are reported. A continuously working integrated sample purification step by ultrafiltration with the membrane cell coupling is established. Using electrical switching valves and separated pumping and eluent systems, the dual HPLC system allows diode array detection as well as measurement of the refractive index. The application of the on-line HPLC monitoring system is demonstrated by measuring the anaerobic H-acid degradation kinetics. H-acid, 1-amino-8-hydroxynaphthalene-3,6-disulfonic acid, is one of the most important coupling components for a variety of direct, mordant, reactive dyes which remains in the process water and the textile dyeing effluents in high concentration.
authors: University of Applied Sciences Cologne, Department of Chemical Engineering and Plant Design, Germany. astrid.rehorek@fh-koeln.de
authors: Astrid
authors: A
authors: Rehorek
authors: Kurt
authors: K
authors: Urbig
authors: Ruth
authors: R
authors: Meurer
authors: Claudia
authors: C
authors: Schäfer
authors: Alexander
authors: A
authors: Plum
authors: Gerd
authors: G
authors: Braun
mesh_terms: D001391:Azo Compounds / Q000737:chemistry*; D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D012031:Refractometry
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D010100:Oxygen