Immunohistochemical evidence for localization of NMDAR1 receptor subunit on dopaminergic neurons of the rat substantia nigra, pars compacta.
Non-fluorescent, double-labeling techniques were used in order to investigate whether NMDAR1 receptor subunits are localized on dopaminergic (i.e. tyrosine hydroxylase-positive) neurons of the rat substantia nigra, pars compacta. It has been found that NMDAR1 receptor subunits are highly abundant in the pars compacta neurons and their dendritic processes. It was also found that vast majority, if not all, of pars compacta neurons which are positive for the presence of NMDAR1 receptor subunits are dopaminergic ones. It is concluded that if NMDAR1 receptor subunits, an indispensable element of functional NMDA receptor ion channel complex, is co-assembled with other subunits of NMDA receptor ion channel complex, NMDA receptors might directly control the activity of dopaminergic neurons.
Wedzony
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Laboratory of Pharmacology and Brain Biostructure, Institute of Pharmacology, Polish Academy of Sciences, Kraków. nfwedzon@cyf-kr.edu.pl
Czepiel
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Fijał
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D000818:Animals; D003712:Dendrites / Q000201:enzymology / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism* / Q000502:physiology; D007150:Immunohistochemistry; D009474:Neurons / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013378:Substantia Nigra / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
C409466:NR1 NMDA receptor; D016194:Receptors, N-Methyl-D-Aspartate; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine