Growth of human glioblastomas as xenografts in the brains of athymic rats.
BACKGROUND
We have developed xenografts of human glioblastoma (GBM) and established the baseline growth parameters and histopathological features of these tumors.


METHODS
Cells from 4 different human GBM cell lines were injected into the right caudate-putamen of brain in athymic rats. We measured tumor weights and the estimated survival time of each rat. RESULTS-CONCLUSION: U-251 MG and U-87 MG cells produced solid intracerebral tumors with a 100% tumor take rate, while SF-767 and SF-126 cells did not grow in the brains of athymic rats. Under the conditions employed, U-87 MG tumors grew faster than U-251 MG tumors, but both types of tumors exhibited reproducible growth characteristics from animal to animal. There was heterogeneity in the growth characteristics and histologies between the 2 tumor types, indicating that these tumor models might be useful for simulating some of the heterogeneity that occurs between GBM in humans.
Ozawa
Tomoko
T
Brain Tumor Research Center, Department of Neurological Surgery, School of Medicine, University of California, San Francisco, California 94143-0520, USA.
Wang
Jingli
J
Hu
Lily J
LJ
Bollen
Andrew W
AW
Lamborn
Kathleen R
KR
Deen
Dennis F
DF
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D008136:Longevity; D008297:Male; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology*; D051381:Rats; D011923:Rats, Nude; D013997:Time Factors; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays / Q000379:methods*