Metabolic underpinnings of the paradoxical net phosphocreatine resynthesis in contracting rat gastrocnemius muscle.
Net phosphocreatine (PCr) resynthesis during muscle contraction is a paradoxical phenomenon because it occurs under conditions of high energy demand. The metabolic underpinnings of this phenomenon were analyzed non-invasively using 31P-magnetic resonance spectroscopy in rat gastrocnemius muscle (n=11) electrically stimulated (7.6 Hz, 6 min duration) in situ under ischemic and normoxic conditions. During ischemic stimulation, [PCr] initially fell to a steady state (9+/-5% of resting concentration) which was maintained for the last 5 min of stimulation, whereas isometric force production decreased to a non-measurable level beyond 3 min. Throughout normoxic stimulation, [PCr] and force production declined to a steady state after respectively 1 min (5+/-3% of resting concentration) and 3.25 min (21+/-8% of initial value) of stimulation. Contrary to the observations under ischemia, a paradoxical net PCr resynthesis was recorded during the last 2 min of normoxic stimulation and was not accompanied by any improvement in force production. These results demonstrate that the paradoxical net PCr resynthesis recorded in contracting muscle relies exclusively on oxidative energy production and could occur in inactivated fibers, similarly to PCr resynthesis during post-exercise recovery.
Giannesini
Benoit
B
Centre de Résonance Magnétique Biologique et Médicale, UMR CNRS 6612, Faculté de Médecine de Marseille, 27 Boulevard Jean Moulin, 13005, Marseille, France.
Izquierdo
Marguerite
M
Cozzone
Patrick J
PJ
Bendahan
David
D
D000255:Adenosine Triphosphate / Q000378:metabolism; D000693:Anaerobiosis; D000818:Animals; D004558:Electric Stimulation; D004734:Energy Metabolism; D006019:Glycolysis; D006614:Hindlimb; D006863:Hydrogen-Ion Concentration; D007537:Isometric Contraction; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010725:Phosphocreatine / Q000096:biosynthesis* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D010725:Phosphocreatine; D000255:Adenosine Triphosphate