title: Gene expression of D-amino acid oxidase in cultured rat astrocytes: regional and cell type specific expression.
abstract: Neuromodulative free D-serine is present in mammalian brain, and localized to type-2 astrocytes in culture. D-amino acid oxidase (DAO) is a flavoenzyme that catalyzes D-amino acids. We examined the DAO gene expression in cultured rat astrocytes by reverse transcriptase-polymerase chain reaction. We established a method to prepare highly purified culture of type-1 and type-2 astrocytes from any brain region. This method utilizes combination of cell type specific separation by shaking and subsequent purification by immunopanning or treatment with cytosine arabinoside. We detected higher DAO gene expression in type-1 astrocyte cultures from cerebellum than that from cerebral cortex. In cerebellum, we observed higher DAO expression in type-1 astrocyte cultures than that in type-2. We also revealed that DAO expression in C6, corresponding to type-1 astrocyte, was higher than that in CG-4 derived type-2 astrocytes.
authors: Department of Gene Regulatorics, Institute for Enzyme Research, The University of Tokushima, Tokushima 770-8503, Japan.
authors: Yoshiteru
authors: Y
authors: Urai
authors: Osamu
authors: O
authors: Jinnouchi
authors: Kyung Tak
authors: KT
authors: Kwak
authors: Atsuhiko
authors: A
authors: Suzue
authors: Shinji
authors: S
authors: Nagahiro
authors: Kiyoshi
authors: K
authors: Fukui
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001253:Astrocytes / Q000166:cytology* / Q000201:enzymology*; D001921:Brain / Q000166:cytology* / Q000201:enzymology*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology; D003561:Cytarabine; D003605:D-Amino-Acid Oxidase / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D000954:Antigens, Surface; D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; C046045:Ran 2 protein, rat; D003561:Cytarabine; D003605:D-Amino-Acid Oxidase