title: Gene expression of 5-epi-aristolochene synthase and formation of capsidiol in roots of Nicotiana attenuata and N. sylvestris.
abstract: Three new copies of a sesquiterpenoid synthase gene encoding 5-epi-aristolochene synthase (EAS) were cloned as cDNAs from Nicotiana attenuata and functionally characterized after expression of the recombinant enzymes in E. coli. Differential patterns of EAS gene expression and formation of the antimicrobial sesquiterpenoid capsidiol were found in roots and shoots of two species of Nicotiana. EAS is expressed constitutively in roots of N. attenuata and N. sylvestris corresponding with constitutive capsidiol formation in roots. Constitutive expression of EAS and capsidiol accumulation were not detectable in shoots of rosette plants of N. attenuata, but accumulation of terpene synthase transcripts could be induced in shoots by feeding of the tobacco hornworm, Manduca sexta. Constitutive expression of EAS has not been previously reported from N. tabacum, where capsidiol is known as an elicitor- and pathogen-inducible phytoalexin. It is conceivable that capsidiol contributes not only to an inducible defense against pathogens, but also to a constitutive defense in an organ-specific manner in some species of Nicotiana.
authors: Max Planck Institute for Chemical Ecology, Winzlaer Strasse 10, Beutenberg Campus, 07745 Jena, Germany. bohlmann@interchange.ubc.ca
authors: Jörg
authors: J
authors: Bohlmann
authors: Einar J
authors: EJ
authors: Stauber
authors: Bernd
authors: B
authors: Krock
authors: Neil J
authors: NJ
authors: Oldham
authors: Jonathan
authors: J
authors: Gershenzon
authors: Ian T
authors: IT
authors: Baldwin
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis / Q000302:isolation & purification; D004790:Enzyme Induction; D004926:Escherichia coli; D008401:Gas Chromatography-Mass Spectrometry; D018506:Gene Expression Regulation, Plant; D018402:Manduca / Q000502:physiology; D008969:Molecular Sequence Data; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism*; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D011235:Predatory Behavior; D011994:Recombinant Proteins; D012717:Sesquiterpenes / Q000032:analysis / Q000378:metabolism*; D014026:Nicotiana / Q000201:enzymology* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D012717:Sesquiterpenes; C081843:capsidiol; D019883:Alkyl and Aryl Transferases; C078217:5-epi-aristolochene synthase