Determination of menadione sodium bisulfite in pharmaceutical preparations by flow-injection on-line photochemical spectrofluorometry.
A flow-injection on-line photochemical spectrofluorometry (FI-PF) was developed for the determination of menadione sodium bisulfite (MSB) using acetone and sodium sulfite as sensitizing reagents. An injected sample band carried by a water stream was on-line merged with a mixed NaOH, Na2SO3 and acetone solution in a "T" connector. It was then driven to pass a knotted PTFE photochemical reactor (0.5 mm i.d. x 200 cm, KR) that was freely coiled around a 6-W low-pressure mercury lamp. While passing the KR, MSB was derived into an intensively fluorescent compound that was on-line delivered into a flow-through cell and detected therein at an emission wavelength of 459 nm and an excitation wavelength of 336 nm. Under optimized conditions a detection limit of 0.38 microg l(-1) was achieved at a sampling rate of 90 h(-1). Eleven determinations of 0.5 mg l(-1) and 0.05 mg l(-1) MSB standard solution gave RSDs of 0.75% and 1.3%, respectively. The calibration curve was linear in the MSB concentration range 0.005-1.5 mg l(-1). The proposed method was successfully applied to assay the MSB content in MSB injection.
He
Q
Q
Department of Chemistry, Zhejiang University, Hangzhou, P R China.
Wang
Z
Z
Cao
X
X
Chen
H
H
Ke
Y
Y
D017022:Flow Injection Analysis / Q000379:methods*; D009862:Online Systems; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010777:Photochemistry / Q000295:instrumentation / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D024483:Vitamin K 3 / Q000032:analysis*
D004364:Pharmaceutical Preparations; D024483:Vitamin K 3