Dipeptidyl peptidase with strict substrate specificity of an anaerobic periodontopathogen Porphyromonas gingivalis.
A dipeptidyl peptidase which hydrolyzed Xaa-Ala-p-nitroanilide was purified to homogeneity by sequential procedures including ammonium sulfate precipitation, ion-exchange chromatography, hydrophobic interaction chromatography, gel filtration and isoelectric focusing from the cell extract of Porphyromonas gingivalis. The purified enzyme hydrolyzed p-nitroanilide derivatives of Lys-Ala, Ala-Ala, and Val-Ala, but not Xaa-Pro. Enzyme activity was maximum at neutral pHs. Its molecular mass was 64 kDa with an isoelectric point of 5.7. The enzyme belonged to the family of serine peptidases.
Fujimura
Setsuo
S
Department of Oral Microbiology, Matsumoto Dental University, Shiojiri-Shi, Nagano-Ken 399-0781, Japan. fujimura@po.mdu.ac.jp
Hirai
Kaname
K
Shibata
Yukinaga
Y
D000409:Alanine / Q000737:chemistry; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002413:Cations, Divalent / Q000494:pharmacology; D003374:Coumarins / Q000494:pharmacology; D004151:Dipeptides / Q000378:metabolism; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D049934:Isocoumarins; D007526:Isoelectric Point; D007531:Isoflurophate / Q000494:pharmacology; D008970:Molecular Weight; D016966:Porphyromonas gingivalis / Q000201:enzymology*; D018720:Prevotella / Q000201:enzymology; D018713:Prevotella intermedia / Q000201:enzymology; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013045:Species Specificity; D013379:Substrate Specificity
D001426:Bacterial Proteins; D002413:Cations, Divalent; D003374:Coumarins; D004151:Dipeptides; D004791:Enzyme Inhibitors; D049934:Isocoumarins; D015842:Serine Proteinase Inhibitors; D007531:Isoflurophate; C046235:3,4-dichloroisocoumarin; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D000409:Alanine