[Screening and characterization of human phage antibody to hepatitis virus C NS5A antigen].
OBJECTIVE
To screen and characterize human phage antibody against hepatitis C virus (HCV)NS5A antigen.


METHODS
The recombinant phages were panned by recombinant HCV NS5A antigen which was coated in a microtiter plate; after five rounds of biopanning, 35 clones were obtained and demonstrated specific to NS5A antigen. The specific binding capacity of the ScFv antibody to HCV NS5A antigen was determined by ELISA.


RESULTS
HCV NS5A phage antibody had a specific combination capacity with hepatitis C virus NS5A antigen. The DNA sequence data showed that the ScFv gene was composed of 789 bp and codes for a peptide of 262 amino acid residues.


CONCLUSIONS
Human single chain antibody to hepatitis C virus NS5A antigen has been identified by means of the phage display technology.
Cheng
J
J
Gene Therapy Research Center, Institute of Infectious Diseases, The 302nd Hospital of PLA, Beijing 100039, China.
Zhong
Y
Y
Shi
S
S
Liu
Y
Y
Wang
G
G
Dong
J
J
Xia
X B
XB
Chen
J M
JM
D000914:Antibodies, Viral / Q000235:genetics*; D016174:Hepacivirus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D019151:Peptide Library; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D057127:Single-Chain Antibodies; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000276:immunology; D014779:Virus Replication
D000914:Antibodies, Viral; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D057127:Single-Chain Antibodies; D017361:Viral Nonstructural Proteins; C114805:scFv fragment SW1; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase