Supercritical fluid extraction and high-performance liquid chromatographic determination of phloroglucinols in St. John's Wort (Hypericum perforatum L.).
A small-scale supercritical fluid extraction (SFE) method was developed for the selective extraction of phloroglucinols from St. John's wort (SJW) leaf/flower mixtures using supercritical carbon dioxide (CO(2)). The extraction efficiency was investigated as influenced by pressure, temperature, time, and modifier. The optimized condition of SFE was carried out at 3.80 x 10(4) kpa (5500 psi) and 50 degrees C. Samples were held in static extraction for 10 min, followed by a dynamic extraction for 90 min at the flow rate of 1 mL/min. A simple and sensitive HPLC method was developed for the analysis of hyperforin and adhyperforin, the major phloroglucinols, in the SFE extract of SJW.
Cui
Yanyan
Y
Division of Chemistry, National Center for Toxicological Research, U.S. Food and Drug Administration, HFT-230, 3900 NCTR Road, Jefferson, Arkansas 72079, USA.
Ang
Catharina Y W
CY
D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D020902:Hypericum / Q000737:chemistry*; D010696:Phloroglucinol / Q000032:analysis*; D012680:Sensitivity and Specificity
D010696:Phloroglucinol