title: Altered dye diffusion and upregulation of connexin37 in mouse aortic endothelium deficient in connexin40.
abstract: Connexin40 (Cx40), connexin37 (Cx37) and connexin43 (Cx43) are subunit proteins of gap junction channels in the vascular wall which are presumably involved in the propagation of vasomotor signals. In this study we have investigated in Cx40-deficient versus wild-type aortic endothelium to which extent loss of Cx40 impairs intercellular communication. We show in Cx40-deficient mice that expression of both Cx37 and Cx43 protein was increased approximately 3- and 2-fold over the level in wild-type endothelium, respectively. Furthermore, Cx37 immunosignals were distributed more homogeneously on contacting plasma membranes in Cx40-deficient versus with wild-type endothelium. Cx43 was not detected in endothelium but only in smooth muscle cells of the vessel wall. Iontophoretic injection of Lucifer Yellow or neurobiotin into aortic endothelium of Cx40-deficient mice showed extensive intercellular transfer of neurobiotin but not of Lucifer Yellow. In contrast, intercellular spreading of Lucifer Yellow was observed in endothelium of wild-type aorta. As shown by electron microscopy, gap junctions in Cx40-deficient endothelium were morphologically different from those of wild-type vessels. These results demonstrate that dye diffusibility of endothelial gap junctions is different in Cx40-deficient and wild-type mice, although Cx40-deficient mice retain the capability of intercellular communication. Apparently, Cx40-deficient endothelial cells upregulate and redistribute Cx37 as a molecular adaptation to the lack of Cx40.
authors: Institut für Genetik, Abteilung Molekulargenetik, Universität Bonn, Deutschland.
authors: Olaf
authors: O
authors: Krüger
authors: Jean-Louis
authors: JL
authors: Bény
authors: Fabienne
authors: F
authors: Chabaud
authors: Otto
authors: O
authors: Traub
authors: Martin
authors: M
authors: Theis
authors: Klaudia
authors: K
authors: Brix
authors: Susanne
authors: S
authors: Kirchhoff
authors: Klaus
authors: K
authors: Willecke
mesh_terms: D000818:Animals; D001011:Aorta; D001710:Biotin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D002450:Cell Communication; D004396:Coloring Agents / Q000378:metabolism*; D018031:Connexin 43 / Q000032:analysis; D017630:Connexins / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D004058:Diffusion; D004730:Endothelium, Vascular / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D017629:Gap Junctions; D015870:Gene Expression; D007478:Iontophoresis; D007546:Isoquinolines / Q000008:administration & dosage / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000737:chemistry; D000097003:Gap Junction alpha-5 Protein; D000097006:Gap Junction alpha-4 Protein
chemical_list: D001710:Biotin; D004396:Coloring Agents; D018031:Connexin 43; D017630:Connexins; D007546:Isoquinolines; D000097003:Gap Junction alpha-5 Protein; D000097006:Gap Junction alpha-4 Protein; C070614:neurobiotin; C017475:lucifer yellow