Proteolysis of CD14 on human gingival fibroblasts by arginine-specific cysteine proteinases from Porphyromonas gingivalis leading to down-regulation of lipopolysaccharide-induced interleukin-8 production.
Arginine-specific cysteine proteinases (gingipains-R) from periodontopathic Porphyromonas gingivalis cleaved CD14, a bacterial pattern recognition receptor, on human gingival fibroblasts (HGF). Consequently, gingipains-R reduced lipopolysaccharide-induced interleukin-8 production by HGF, indicating that gingipains-R inhibited CD14-dependent HGF activation and are involved in immune evasion by the bacterium in periodontal tissues.
Tada
Hiroyuki
H
Department of Microbiology and Immunology, Tohoku University School of Dentistry, Sendai 980-8575, Japan.
Sugawara
Shunji
S
Nemoto
Eiji
E
Takahashi
Nobuhiro
N
Imamura
Takahisa
T
Potempa
Jan
J
Travis
James
J
Shimauchi
Hidetoshi
H
Takada
Haruhiko
H
D036541:ADP-ribosyl Cyclase; D018829:Adhesins, Bacterial; D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D015703:Antigens, CD; D001120:Arginine; D019013:CD40 Antigens / Q000096:biosynthesis; D018957:CD59 Antigens / Q000096:biosynthesis; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases / Q000276:immunology* / Q000494:pharmacology; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D015536:Down-Regulation / Q000276:immunology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D058851:GPI-Linked Proteins; D000080867:Gingipain Cysteine Endopeptidases; D005881:Gingiva / Q000166:cytology / Q000276:immunology*; D006388:Hemagglutinins / Q000276:immunology* / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D016966:Porphyromonas gingivalis / Q000201:enzymology*
D018829:Adhesins, Bacterial; D000590:Amino Acid Chloromethyl Ketones; D015703:Antigens, CD; D019013:CD40 Antigens; D018957:CD59 Antigens; D015853:Cysteine Proteinase Inhibitors; D058851:GPI-Linked Proteins; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins; D016209:Interleukin-8; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D001120:Arginine; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2; D003546:Cysteine Endopeptidases; C040308:phenylalanyl-prolyl-arginine-chloromethyl ketone