Cooperative repression of cyclin-dependent kinase inhibitor p21 gene expression by hepatitis B virus X protein and hepatitis C virus core protein.
Co-infection with hepatitis B virus (HBV) and hepatitis C virus (HCV) is common and is associated with a more severe liver disease and increased frequency in the development of hepatocellular carcinoma (HCC). Here, we demonstrated that HBV X protein (HBx) and HCV core protein additively repress the universal cyclin-dependent kinase inhibitor p21 gene at the transcription level. The transforming growth factor-beta responsive element and Sp1 site of the p21 promoter were responsible for the effect of HCV core and HBx, respectively. Furthermore, cell growth was additively stimulated by them, suggesting that additive repression of the p21 might be important to understand the cooperative development of HCC by HBV and HCV.
Han
Hae Jin
HJ
Department of Microbiology, College of Natural Sciences, Pusan National University, 609-735, Pusan, South Korea.
Jung
Eun Young
EY
Lee
Woo Jung
WJ
Jang
Kyung Lib
KL
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000821:virology; D002455:Cell Division; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000096:biosynthesis / Q000235:genetics*; D020868:Gene Silencing; D007700:Kinetics; D008113:Liver Neoplasms / Q000821:virology; D051379:Mice; D011401:Promoter Regions, Genetic; D012313:RNA / Q000096:biosynthesis; D020218:Response Elements; D016329:Sp1 Transcription Factor / Q000378:metabolism; D015534:Trans-Activators / Q000494:pharmacology*; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000502:physiology; D014758:Viral Core Proteins / Q000494:pharmacology*; D054334:Viral Regulatory and Accessory Proteins
C493957:Cdkn1a protein, mouse; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D016329:Sp1 Transcription Factor; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; D014758:Viral Core Proteins; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; C069130:nucleocapsid protein, Hepatitis C virus; D012313:RNA