Reductive deamination as a new step in the anaerobic microbial degradation of halogenated anilines.
In this paper we report the isolation and characterization of an anaerobic enrichment culture as well as of a Rhodococcus sp. strain 2 capable of degrading 3,4-dihaloanilines under nitrate reducing conditions. Using mass spectrometry several of the intermediates formed in the process of 3,4-dichloroaniline conversion were identified. Most interesting is the observation of reductive deamination and the formation of 1,2-dichlorobenzene as one of the intermediates. Using 19F NMR and fluorinated 3,4-dihaloaniline model substrates it was corroborated that reductive deamination of the anilines to give dihalobenzene intermediates represents a new initial step in the anaerobic microbial degradation of these halogenated anilines.
Travkin
Vasili
V
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russian Federation.
Baskunov
Boris P
BP
Golovlev
Eugene L
EL
Boersma
Marelle G
MG
Boeren
Sjef
S
Vervoort
Jacques
J
van Berkel
Willem J H
WJ
Rietjens
Ivonne M C M
IM
Golovleva
Ludmila A
LA
D000693:Anaerobiosis; D000814:Aniline Compounds / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004785:Environmental Pollutants / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012240:Rhodococcus / Q000254:growth & development / Q000378:metabolism*
D000814:Aniline Compounds; D004785:Environmental Pollutants; C014464:3,4-dichloroaniline; C025654:3-chloro-4-fluoroaniline