Intralesion transplantation of serotonergic precursors enhances locomotor recovery but has no effect on development of chronic central pain following hemisection injury in rats.
The effects of intralesion grafts of serotonergic precursors on locomotor recovery and development of chronic pain were assessed after chronic spinal cord hemisection injury (SCI) in rats. Serotonin- and brain-derived neurotrophic factor-secreting (RN46A-B14) and RN46A-vector-only cells were transplanted into the site of T13 lateral hemisection 10 days following injury in immunosuppressed animals, and locomotor and pain related behaviors were assessed weekly for 28 days. There were significant improvements in the degree of spontaneous locomotor recovery, but no significant difference was found in the magnitude of development of mechanical allodynia or thermal hyperalgesia in any transplant group. From these results, we conclude that intraparenchymal engraftment of RN46A-B14 cells is largely ineffective in influencing somatosensory outcomes after SCI, in contrast with the efficacy of dorsal intrathecal placement.
Hains
Bryan C
BC
Department of Anatomy and Neurosciences, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-1043, USA.
Yucra
Jennifer A
JA
Eaton
Mary J
MJ
Hulsebosch
Claire E
CE
D000818:Animals; D016380:Brain Tissue Transplantation / Q000379:methods*; D002478:Cells, Cultured; D002908:Chronic Disease; D020233:Gait Disorders, Neurologic / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery*; D007278:Injections, Spinal; D008297:Male; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D010146:Pain / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery*; D010147:Pain Measurement; D017288:Pain Threshold / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000502:physiology; D020127:Recovery of Function / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism*; D013116:Spinal Cord / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013346:Subarachnoid Space / Q000601:surgery; D016896:Treatment Outcome
D012701:Serotonin