Fuc(alpha1-->3)GalNAc-: the major antigenic motif of Schistosoma mansoni glycolipids implicated in infection sera and keyhole-limpet haemocyanin cross-reactivity.
The aim of the present study was the characterization of the dominant epitope present on Schistosoma mansoni glycolipids, which causes cross-reactivity of S. mansoni and S. haematobium infection sera with keyhole-limpet haemocyanin (KLH). To this end, the monoclonal antibody M2D3H was chosen for its similar behaviour in high-performance TLC immunostaining and inhibition-ELISA to infection sera. Individual, structurally defined oligosaccharides derived from S. mansoni egg glycolipids were tested for their binding to this monoclonal antibody by immunoaffinity chromatography. A terminal fucose residue linked in the (alpha1-->3) position to N-acetylgalactosamine was found to be the common structural determinant of the four oligosaccharides binding to M2D3H. The Fuc(alpha1-->3)GalNAc-motif also appeared to be the basis for the cross-reactivity with KLH, a phenomenon used in the serodiagnosis of S. mansoni, S. haematobium and S. japonicum infections.
Kantelhardt
Sven R
SR
Institute of Biochemistry, Faculty of Medicine, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany.
Wuhrer
Manfred
M
Dennis
Roger D
RD
Doenhoff
Michael J
MJ
Bickle
Quentin
Q
Geyer
Rudolf
R
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000737:chemistry*; D020816:Amino Acid Motifs; D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D012550:Schistosoma mansoni / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D000941:Antigens; D006017:Glycolipids; D009844:Oligosaccharides; D011134:Polysaccharides; C028011:fucosyl alpha 1,3 N-acetylglucosamine; D000117:Acetylglucosamine