Changes in fused cells induced by hvj (sendai virus): redistribution of cytoplasmic organelles and cytoskeletal reorganization.
To understand the relationship between the location of organelles and cellular function, we examined the dynamic state of cytoplasmic organelles and cytoskeleton in polynuclear Ehrlich ascites tumor (EAT) cells fused with hemagglutinating virus of Japan (HVJ; Sendai virus) by confocal laser scanning microscopy. Irregular fused cells gradually became spherical during culture, and nuclei and mitochondria were redistributed in the fused cell; nuclei formed a cluster surrounded by mitochondria. F-actin, vimentin, and microtubules were also reorganized with the redistribution of cell organelles. Further, when the morphological change was inhibited by L4-1, a chlorophyll-like substance derived from silkworm faeces, or pyropheophorbide-a, the arrangement of organelles and cytoskeleton remained disturbed, suggesting that the movement of the cytoskeleton is closely associated with cell shape and the distribution of cytoplasmic organelles.
Hirayama
Etsuko
E
Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, 1, Shichonocho, Misasagi, Yamashina-ku 607-8412, Japan.
Ama
Masako
M
Takahashi
Ken-Ichiro
K
Hiraki
Akihiro
A
Kim
Jeman
J
D000199:Actins / Q000502:physiology; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology / Q000503:physiopathology; D003599:Cytoskeleton / Q000502:physiology*; D006822:Hybrid Cells / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D018613:Microscopy, Confocal; D015388:Organelles / Q000502:physiology*; D011166:Porphyrins / Q000502:physiology; D029082:Sendai virus / Q000502:physiology / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D000199:Actins; D011166:Porphyrins