title: Development of radioimmunoassay for measurement of serum digoxin in digitalized patients using novel anti-digoxin antiserum.
abstract: There is an antiserum elicited by digoxin 3'-hemisuccinate-bovine serum albumin (BSA) conjugate possessing high specificity for digoxin. Our study focused on development of RIA using this novel antiserum for measurement of digoxin in serum from digitalized patients. The property of the new antiserum was investigated by RIA with digoxin 3'-hemisuccinyl-[3H]leucine. The separation of bound and free fractions was performed using a dextran-coated charcoal suspension. The new antiserum bound approximately 50% of digoxin 3'-hemisuccinyl-[3H]leucine with a final dilution of 1:30000. The intra- and inter-assay coefficients of variation were <9% in the range of 0.52-4.17 ng/ml. The mean digoxin concentration in serum samples (n=35) from digitalized patients was estimated to be 0.68 ng/ml, which was lower than its measurement of digoxin with the commercial antidigoxin BSA serum and monoclonal anti-digoxin. It is apparent that the RIA described here has sufficient precision. The RIA system was available for the measurement of digoxin in serum from digitalized patients.
authors: Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan. y-ikeda@hokuriku-u.ac.jp
authors: Yukari
authors: Y
authors: Ikeda
authors: Tsutomu
authors: T
authors: Araki
authors: Hiroaki
authors: H
authors: Takimoto
authors: Youichi
authors: Y
authors: Fujii
mesh_terms: D000818:Animals; D002417:Cattle; D004077:Digoxin / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood*; D006801:Humans; D007106:Immune Sera / Q000032:analysis / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods* / Q000706:statistics & numerical data; D012710:Serum Albumin, Bovine / Q000032:analysis
chemical_list: D007106:Immune Sera; C085465:digoxin 3'-hemisuccinate-bovine serum albumin conjugate; D012710:Serum Albumin, Bovine; D004077:Digoxin