Growth-associated protein 43 is up-regulated in the ganglion cells of the ischemic rat retina.
We investigated the expression and cellular localization of growth-associated protein (GAP)-43 in the rat retina following ischemia induced by transiently increased intraocular pressure. In the normal retina, GAP-43 immunoreactivity was restricted to profiles in the inner plexiform layer. Following ischemia and reperfusion, immunoreactivity appeared in ganglion cells. The cell density of labeled ganglion cells peaked three days post-lesion and then decreased at seven days. Quantitative evaluation by immunoblotting confirmed that GAP-43 expression increased at three days (to 190% of control levels) and then slightly decreased at seven days. Our findings suggest that some ganglion cells have the potential to regenerate through the up-regulation of GAP-43 in the ischemic rat retina.
Ju
Won-Kyu
WK
Department of Anatomy, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, Seoul 137-701, Korea.
Gwon
Jae-Sung
JS
Park
Sung-Jin
SJ
Kim
Keun-Young
KY
Moon
Jung-Il
JI
Lee
Mun-Yong
MY
Oh
Su-Ja
SJ
Chun
Myung-Hoon
MH
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000503:physiopathology; D016923:Cell Death / Q000502:physiology*; D019922:GAP-43 Protein / Q000378:metabolism*; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D007429:Intraocular Pressure / Q000502:physiology; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology; D012164:Retinal Diseases / Q000378:metabolism* / Q000503:physiopathology; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism*; D013477:Superior Colliculi / Q000166:cytology / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology*; D014795:Visual Pathways / Q000166:cytology / Q000378:metabolism
D019922:GAP-43 Protein; D006735:Horseradish Peroxidase