title: One-step PCR to distinguish B virus from related primate alphaherpesviruses.
abstract: By adding betaine to the PCR mixture, we previously established a PCR method to amplify a DNA segment of the glycoprotein G gene of B virus (BV) derived from a rhesus macaque. We have found that DNA of other BV strains derived from cynomolgus, pigtail, and lion-tailed macaques can also serve as the template in our PCR assay. Under the same conditions no product was obtained with DNA of simian agent 8 of green monkeys and Herpesvirus papio 2 of baboons, or the human herpes simplex viruses types 1 and 2. Thus, this PCR method is useful to discriminate BV from other closely related primate alphaherpesviruses.
authors: Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
authors: Makoto
authors: M
authors: Hirano
authors: Shin
authors: S
authors: Nakamura
authors: Fusako
authors: F
authors: Mitsunaga
authors: Maki
authors: M
authors: Okada
authors: Shuya
authors: S
authors: Shirahama
authors: Richard
authors: R
authors: Eberle
mesh_terms: D018138:Alphaherpesvirinae / Q000145:classification* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001622:Betaine; D002522:Chlorocebus aethiops; D006566:Herpesviridae Infections / Q000662:veterinary / Q000821:virology*; D006568:Herpesvirus 1, Cercopithecine / Q000145:classification* / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000145:classification / Q000235:genetics; D018258:Herpesvirus 2, Human / Q000145:classification / Q000235:genetics; D006801:Humans; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D010215:Papio; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011323:Primates; D014709:Vero Cells
chemical_list: D001622:Betaine