[Hepatocyte targeted DNA carrier L-PEI: the lactosylated polyethyleneimine and transfection in vitro].
BACKGROUND
To develop a hepatocyte targeted DNA carrier for expression of IFN or delivery of antiviral antisense neucleic acid into hapatocytes.


METHODS
The hepatocyte targeted lactosylated polyethleneimine (L-PEI) was prepared through reductive amination of PEI with lactose. The in vitro transfection experiment was conducted and the stability of the DNA complex in rat serum was evaluated.


RESULTS
L-PEI can effectively deliver luciferase gene into the galactose receptor positive Huh-7 cells. The transfection can be specifically inhibited by the synthesized ligand-lactosaminated BSA. The complex of DNA/L-PEI is stable in rat serum with only little degradation observed after 30 minutes, while free DNA was destroyed completely.


CONCLUSIONS
L-PEI can be used as an specific and efficient DNA carrier.
Jin
X
X
he 302th Hospital of PLA, Beijing 100039, China.
Zhang
L
L
Lou
M
M
D000818:Animals; D004247:DNA / Q000008:administration & dosage* / Q000378:metabolism; D004337:Drug Carriers; D018390:Gene Targeting; D022781:Hepatocytes / Q000378:metabolism*; D066298:In Vitro Techniques; D011094:Polyethyleneimine / Q000737:chemistry*; D051381:Rats; D014162:Transfection
D004337:Drug Carriers; D011094:Polyethyleneimine; D004247:DNA