Protein kinases activities in erythrocyte membranes of asphyxiated newborns.
OBJECTIVE
Perinatal asphyxia represents a major cause of acute brain impairment and mortality in neonates. To develop the effective therapies able to reduce post-asphyxial damages, the understanding of biochemical processes accompanying asphyxia appears to be of the great relevance.


METHODS
The activities of protein kinases A and C, and tyrosine kinases in erythrocyte membranes of healthy and asphyxiated neonatals were compared. Using monoclonal antibodies the band 3 presence and its phosphotyrosine levels were assayed.


RESULTS
In asphyxiated erythrocyte membranes the activities of PKA and tyrosine kinases increased, whereas the activity of PKC was reduced in relation to healthy newborns. Under asphyxia the band 3 has been overphosphorylated; however, its amount decreased.


CONCLUSIONS
These findings may provide some evidence for a potential role of asphyxia in disturbance of phosphorylation processes in erythrocytes, as reflected by altered protein kinases activities. The diminished band 3 presence may be partially responsible for the impairment of erythrocyte function.
Zylinska
Ludmila
L
Neurochemical Laboratory, Department of Biochemistry, Medical University of Lodz, Poland. luska@csk.am.lodz.pl
Sobolewska
Barbara
B
Gulczynska
Ewa
E
Ochedalski
Tomasz
T
Soszynski
Miroslaw
M
D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D001238:Asphyxia Neonatorum / Q000097:blood* / Q000201:enzymology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D006801:Humans; D015151:Immunoblotting; D007231:Infant, Newborn; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000032:analysis; D011493:Protein Kinase C / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*
D001457:Anion Exchange Protein 1, Erythrocyte; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C