[Discovery of natural variant strain of HIV1 in env gene C2-V3 encoded PND of membrane protein].
OBJECTIVE
To sequence and analyze the env gene C2-V3 region of proviral genome from a HIV1 isolate(WWBH7) which was obtained from Huadong Area in China.


METHODS
The env gene C2-V3 DNA fragment was amplified by nested-primer PCR with genome DNA of eripheral blood mononuclear cells from a confirmed HIV1 infected individual as a template. The amplified DNA fragment was inserted into pGEM-T vector. The recombinant plasmid was confirmed by restriction enzyme analysis. The inserted DNA fragment was sequenced by ABI737 autosequencer and analyzed by PROSIS software.


RESULTS
The HIV1 strain was the derivatives of HIV1 B subtype. But there was mutation of 192 bp fragment repeated insertion at env C2-V3 region of the HIV1 strain compared with standard HIV1 B subtype such as SF2 strain. The mutation brought about a double V3 region in gene encoded PND (principal neutralizing domains). The DNA sequence was registered in GenBank (AF220245).


CONCLUSIONS
This was a natural mutated variant strain of HIV1 whose genome showed a 192 bp repeated insertion at C2-V3 region of env gene encoded PND of membrane protein.
Wang
B
B
Laboratory of Molecular Virology, Medical College of Qingdao University, Qingdao 266021, China.
Fu
J
J
Wu
H
H
Liu
C X
CX
Qian
D M
DM
Fang
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D015700:HIV Envelope Protein gp41 / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D017422:Sequence Analysis, DNA
D015700:HIV Envelope Protein gp41