Effect of a trapping force on a photon-counting histogram.
A theoretical study of the effect of a harmonic trapping force on the distribution of the fluorescence photon counts from Brownian particles is reported. The research of by Chen et al. [Biophys. J. 77, 553 (1999)] is extended. Simulations of the photon-counting histograms indicate that the trapping force parameters can be estimated from changes in the shapes of the histograms, at least for microspheres.
Chirico
Giuseppe
G
Istituto Nazionale per la Fisica della Materia, Dipartamento di Fisica, Università di Milano Bicocca, Milan, Italy. giuseppe.chirico@mib.infn.it