title: Analysis of experimental liver metastasis from tumors inoculated in the mouse stomach cavity.
abstract: OBJECTIVE
  This study was conducted to develop a patient-like hematogenous metastatic model of gastric-cancer in order to gain an understanding of the tumor biology and to search for new methods of treatment.
  
  
  METHODS
  We established a natural and easily reproducible liver metastasis model by orthotopic gastric inoculation in Balb/c mice, using the syngeneic tumor, colon 26.
  
  
  RESULTS
  This model allowed us to evaluate the effect of partial gastric resection with excision of tumor nodules on the formation of experimental liver metastases from the stomach cavity. Mice given partial gastrectomy showed less metastatic ability than control mice. In these experimental groups, liver metastasis was observed in the only group of mice that died of local tumor regrowth due to incomplete resection of the primary tumor. It is suggested that a period of at least 10 days is required for the formation of liver metastases after tumor inoculation into the stomach cavity. There was no significant increase in the number of liver metastases following splenectomy, or after the administration of anti-asialo GM1 antibody or silica.
  
  
  CONCLUSIONS
  This experimental model of liver metastases will provide a useful means of understanding tumor biology and the regulation of liver metastases by host immunocompetent cells, and for assessing new therapeutic agents.
authors: Department of Academic Surgery, Graduate School of Medicine, Chiba University, Japan.
authors: Yoshio
authors: Y
authors: Gunji
authors: Serdar
authors: S
authors: Yol
authors: Takao
authors: T
authors: Suzuki
authors: Seiji
authors: S
authors: Hori
authors: Hideki
authors: H
authors: Hayashi
authors: Hideaki
authors: H
authors: Shimada
authors: Hisahiro
authors: H
authors: Matsubara
authors: Yoshihiro
authors: Y
authors: Nabeya
authors: Takenori
authors: T
authors: Ochiai
mesh_terms: D000818:Animals; D005260:Female; D005743:Gastrectomy; D008113:Liver Neoplasms / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000473:pathology* / Q000601:surgery; D014407:Tumor Cells, Cultured