title: Recognition of synthetic polypeptides corresponding to the N- and C-terminal fragments of Plasmodium falciparum Exp-1 by T-cells and plasma from human donors from African endemic areas.
abstract: The present work describes the recognition of three synthetic polypeptides encompassing the N- and C-terminal regions of the transmembrane Exp-1 protein of the parasite Plasmodium falciparum by plasma and peripheral blood mononuclear cells from naturally exposed individuals living in African endemic areas. The three polypeptides comprise the sequences 23-105, 73-162 and 101-162, and overlap at the transmembrane domain (73-105). Thus, they permitted characterization of the immune response specific to the N- and C-terminal domains in an independent fashion. Two different populations were evaluated, one in the village of Safo in Mali and the other in the villages of Somnaway, Kabortenga and Toussouktenga in Burkina Faso. Antibodies to the sequence 73-162 of Pf Exp-1 were found in 70% of adult Mali donors and in all of the donors tested from Burkina Faso. Strikingly, the N-terminal fragment Pf Exp-1 23-105 was only weakly recognized by a few donors. Evaluation of the T-cell response indicated that the peptide Pf Exp-1 23-105 was more potent than Pf Exp-1 73-162 in inducing a proliferative response. A correlation between peptide-specific interferon-gamma and interleukin-6 production and proliferation to peptide Pf Exp-1 23-105 was observed. Further studies are needed to evaluate this molecule as a vaccine candidate.
authors: Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
authors: Valentin
authors: V
authors: Meraldi
authors: Issa
authors: I
authors: Nebié
authors: Rémy
authors: R
authors: Moret
authors: Nadine
authors: N
authors: Cuzin-Ouattara
authors: Ali
authors: A
authors: Thiocone
authors: Ogobara
authors: O
authors: Doumbo
authors: Fulvio
authors: F
authors: Esposito
authors: Alfred S
authors: AS
authors: Traoré
authors: Giampietro
authors: G
authors: Corradin
authors: Silvia
authors: S
authors: Terenzi
mesh_terms: D000293:Adolescent; D000328:Adult; D000349:Africa / Q000453:epidemiology; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology*; D000953:Antigens, Protozoan / Q000737:chemistry* / Q000276:immunology*; D002050:Burkina Faso / Q000453:epidemiology; D002478:Cells, Cultured; D002675:Child, Preschool; D019353:Endemic Diseases; D005260:Female; D006801:Humans; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000276:immunology*; D008297:Male; D008302:Mali / Q000453:epidemiology; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D010446:Peptide Fragments; C063362:QF116 antigen, Plasmodium falciparum