title: Identification of nucleolin and nucleophosmin as genotoxic stress-responsive RNA-binding proteins.
abstract: Genotoxic stress (DNA damage) can elicit multiple responses in mammalian cells, including the activation of numerous cascades of signal transduction that result in the activation of cellular genes involved in growth control, DNA repair and apoptosis. In an earlier report, we have shown that DNA-damaging agents can also induce the RNA-binding activity of several specific proteins that favor a double stem-loop RNA structure. Here we report the purification and identification of nucleophosmin (NPM) and nucleolin as two genotoxic stress-responsive RNA-binding proteins. UV radiation induces the protein expression levels and RNA-binding activity of NPM while nucleolin RNA-binding activity increases after UV or ionizing radiation exposure. Moreover, we have identified 40 mRNA ligands that are potentially regulated by nucleolin, several of which are stress-responsive transcripts. In addition, our data indicate that activation of nucleolin RNA-binding activity by genotoxic stress is mediated by stress-activated protein kinase p38. Our findings suggest that activation of the RNA-binding properties of nucleolin and NPM is part of the cellular response to genotoxic stress.
authors: University of Maryland, Baltimore, School of Medicine, Biochemistry and Molecular Biology Department, 108 North Greene Street, Baltimore, MD 21201-1503, USA.
authors: Chonglin
authors: C
authors: Yang
authors: Dony A
authors: DA
authors: Maiguel
authors: France
authors: F
authors: Carrier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D004249:DNA Damage; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009153:Mutagens / Q000494:pharmacology; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding / Q000187:drug effects / Q000528:radiation effects; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D048051:p38 Mitogen-Activated Protein Kinases; D000097796:Nucleolin
chemical_list: D008741:Methyl Methanesulfonate; D020928:Mitogen-Activated Protein Kinases; D009153:Mutagens; D009687:Nuclear Proteins; D000090243:Nucleophosmin; D010750:Phosphoproteins; D012313:RNA; D016601:RNA-Binding Proteins; D048051:p38 Mitogen-Activated Protein Kinases; D000097796:Nucleolin; C000716967:NPM1 protein, human