Synthesis and ion conductance behavior of a tetrameric alamethicin ion channel.
[structure: see text] A porphyrin-tethered construct, containing four full-length alamethicin monomers, has been synthesized and characterized. The ion conductance data of the assembly in 1 M HCl display long-lived, albeit noisy, channels that appear to be voltage-independent multiples of only one conductance state. The noise in the data is consistent with the molecular modeling studies, which indicate that the side chain of glutamine 7 of alamethicin does not fit well into the narrow pore of a parallel four-helix bundle.
Wassner
Ari J
AJ
Department of Chemistry, Haverford College, 370 Lancaster Avenue, Haverford, Pennsylvania 19041, USA.
Hurt
Jessica A
JA
Lear
James D
JD
Akerfeldt
Karin S
KS
D000408:Alamethicin / Q000138:chemical synthesis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D007473:Ion Channels / Q000737:chemistry*; D007476:Ionophores / Q000138:chemical synthesis* / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013698:Templates, Genetic
D007202:Indicators and Reagents; D007473:Ion Channels; D007476:Ionophores; D000408:Alamethicin