title: [Determination of levamisole in livestock products using high performance liquid chromatography].
abstract: A method is described for the determination of the anthelmintic levamisole in muscle, liver, kidney and fat of cattle, swine and poultry using high performance liquid chromatography with photodiode array detection. Levamisole was extracted from an alkaline sample with ethyl acetate and back-extracted with 0.1 mol/L hydrochloric acid. The extract was applied to an SCX solid-phase extraction column. The column was washed with water and methanol. Levamisole was eluted with a solution of ammonia in methanol. The eluate was evaporated to dryness and the residue was dissolved in the mobile phase and injected into the HPLC system. Mean recoveries from 0.01-0.10 microgram/g fortified muscle, liver, kidney and fat samples ranged from 78.3 to 99.8%. The detection limit for the assay was 0.005 microgram/g.
authors: Tokyo Metropolitan Research Laboratory of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
authors: Miho
authors: M
authors: Sakamoto
authors: Kazue
authors: K
authors: Takeba
authors: Kenji
authors: K
authors: Fujinuma
authors: Katsuhiko
authors: K
authors: Jimbo
authors: Tomoyuki
authors: T
authors: Miyazaki
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000032:analysis*; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007978:Levamisole / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D013552:Swine
chemical_list: D000969:Antinematodal Agents; D007978:Levamisole