title: Urinary 8-hydroxydeoxyguanosine and sister chromatid exchanges in patients with total hip replacements.
abstract: Ion release from metal implants has been suspected to increase the risk of genotoxic effects in patients wearing orthopedic metal devices. In this study we used urinary 8-hydroxydeoxyguanosine (8-OHdG) as marker of oxidative DNA damage and the frequency of sister chromatid exchanges in lymphocytes to test a possible relationship between the concentrations of chromium or cobalt and the induction of cytogenetic modifications in 46 patients with total hip replacements. A broad range of individual levels of metals has been observed in these patients: chromium in blood, 1.59-14.11 microg/L; chromium in urine, 0.79-93.80 microg/24 h; cobalt in blood, 0.77-37.80 microg/L; cobalt in urine, 2.59-166.94 microg/24 h. By linear regression analysis, no significant correlation between urinary 8OHdG or sister chromatid exchange (SCE) and the concentrations of metals was found. However, cobalt in blood as well as 8-OHdG in urine were higher in patients with implants 3-4 yr old as compared to patients with implants 1-2 yr old. Smoking significantly increased the frequency of SCE. Our data do not indicate a dependence of 8-OHdG in urine or SCE on the levels of chromium or cobalt in patients with total hip replacements.
authors: Department of Occupational Medicine, University of Vienna, Austria. alex.pilger@akh-wien.ac.at
authors: Alexander
authors: A
authors: Pilger
authors: Andreas
authors: A
authors: Schaffer
authors: Hugo W
authors: HW
authors: Rüdiger
authors: Wolf
authors: W
authors: Osterode
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D019644:Arthroplasty, Replacement, Hip; D002857:Chromium / Q000097:blood / Q000652:urine; D003035:Cobalt / Q000097:blood / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D005260:Female; D006622:Hip Prosthesis / Q000009:adverse effects*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D012907:Smoking / Q000378:metabolism; D013997:Time Factors
chemical_list: D002857:Chromium; D003035:Cobalt; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine