Evaluation of drug-induced hepatotoxicity by plasma retinol binding protein.
Retinol binding protein (RBP) in the plasma of rats treated with D-galactosamine was monitored to establish whether its level can be used to evaluate drug-induced hepatotoxicity. Blood was withdrawn by heart puncture at 0 hours and 12 hours after the administration of D-galactosamine (400 mg/kg body weight i.p.) to rats. Lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) in the plasma at 12 hours after the D-galactosamine administration significantly increased, while RBP in the plasma at that time significantly decreased. On the other hand, the albumin in the plasma was unaffected at 12 hours after the D-galactosamine administration. Thus RBP seems to monitor different aspects of drug-induced hepatotoxicity than LDH and ALT and to detect the drug-induced hepatotoxicity more sensitively than albumin level under the present conditions.
Isozaki
Masanao
M
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cyo, Inage-ku, Chiba 263-8522, Japan.
Masubuchi
Yasuhiro
Y
Horie
Toshiharu
T
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood* / Q000473:pathology; D005688:Galactosamine / Q000008:administration & dosage / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D007770:L-Lactate Dehydrogenase / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012177:Retinol-Binding Proteins / Q000378:metabolism*; D054839:Retinol-Binding Proteins, Plasma; D012709:Serum Albumin / Q000032:analysis
D012177:Retinol-Binding Proteins; D054839:Retinol-Binding Proteins, Plasma; D012709:Serum Albumin; D005688:Galactosamine; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase