title: Abscisic acid- and cold-induced thaumatin-like protein in winter wheat has an antifungal activity against snow mould, Microdochium nivale.
abstract: Cold acclimation of winter wheat (Triticum aestivum L.) seedlings induces accumulation in the apoplast of taTLPs that are similar to thaumatin-like proteins (TLPs), which are pathogenesis-related proteins. We characterized a cDNA of WAS-3a encoding the major isoform of taTLPs from winter wheat cells and showed that WAS-3a transcripts were markedly increased by treatment with ABA and by treatment with elicitors (chitosan, beta-glucan and cell wall fractions of Fusarium oxysporum and Microdochium nivale) in wheat cells. To analyse the function of WAS-3a, a highly efficient expression system using wheat cells was established, and a large amount of recombinant WAS-3a protein (rWAS-3a) was obtained with near homogeneity. Antifungal assays using various fungi grown on agar plates revealed that rWAS-3a inhibits hyphal growth of pink snow mould, Microdochium nivale, at a low temperature. The results suggest that cold-induced taTLPs that accumulate in the apoplast contribute to snow mould resistance of winter wheat.
authors: Institute of Low Temperature Science, Hokkaido University, Kita-19 Nishi-8, Kita-ku, Sapporo 060-0819, Japan Research Institute of Agricultural Resources, Ishikawa Agricultural College, 1-308, Suematsu, Nonoichi, Ishikawa 921, Japan Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-8, Kita-ku, Sapporo, 060-8589, Japan.
authors: Chikako
authors: C
authors: Kuwabara
authors: Daisuke
authors: D
authors: Takezawa
authors: Takiko
authors: T
authors: Shimada
authors: Tatsurou
authors: T
authors: Hamada
authors: Seizo
authors: S
authors: Fujikawa
authors: Keita
authors: K
authors: Arakawa