title: Post-termination complex disassembly by ribosome recycling factor, a functional tRNA mimic.
abstract: Ribosome recycling factor (RRF) together with elongation factor G (EF-G) disassembles the post- termination ribosomal complex. Inhibitors of translocation, thiostrepton, viomycin and aminoglycosides, inhibited the release of tRNA and mRNA from the post-termination complex. In contrast, fusidic acid and a GTP analog that fix EF-G to the ribosome, allowing one round of tRNA translocation, inhibited mRNA but not tRNA release from the complex. The release of tRNA is a prerequisite for mRNA release but partially takes place with EF-G alone. The data are consistent with the notion that RRF binds to the A-site and is translocated to the P-site, releasing deacylated tRNA from the P- and E-sites. The final step, the release of mRNA, is accompanied by the release of RRF and EF-G from the ribosome. With the model post-termination complex, 70S ribosomes were released from the post-termination complex by the RRF reaction and were then dissociated into subunits by IF3.
authors: Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors: Go
authors: G
authors: Hirokawa
authors: Michael C
authors: MC
authors: Kiel
authors: Aiko
authors: A
authors: Muto
authors: Maria
authors: M
authors: Selmer
authors: V Samuel
authors: VS
authors: Raj
authors: Anders
authors: A
authors: Liljas
authors: Kazuei
authors: K
authors: Igarashi
authors: Hideko
authors: H
authors: Kaji
authors: Akira
authors: A
authors: Kaji
mesh_terms: D004926:Escherichia coli; D046911:Macromolecular Substances; D010443:Peptide Chain Termination, Translational / Q000502:physiology*; D020653:Peptide Elongation Factor G / Q000037:antagonists & inhibitors / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011506:Proteins / Q000037:antagonists & inhibitors / Q000502:physiology*; D012333:RNA, Messenger / Q000502:physiology*; D012343:RNA, Transfer / Q000502:physiology*; D012269:Ribosomal Proteins; D012270:Ribosomes / Q000502:physiology
chemical_list: D046911:Macromolecular Substances; D020653:Peptide Elongation Factor G; D011500:Protein Synthesis Inhibitors; D011506:Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C087267:ribosome releasing factor; D012343:RNA, Transfer