title: Non-coordinate expression of closely linked mouse casein genes.
abstract: Expression levels of five mouse casein genes were analysed in the mammary gland of virgin, pregnant and lactating mice. We have already shown that the five murine casein genes are arranged in the order, alpha-beta-gamma-epsilon-kappa in a tandem array, very close to each other in a 250 kb DNA fragment of mouse genome. Northern blot analysis showed that, of the calcium-sensitive casein genes, the epsilon casein gene is expressed only during lactation unlike the alpha, beta and gamma casein genes which are expressed during pregnancy and lactation. Even though the alpha, beta and gamma genes exhibited a co-ordinated expression pattern from mid to the later stages of pregnancy, the mRNA levels varied considerably (60, 90 and 100% respectively) by the onset of lactation. The mRNA level of the calcium-insensitive kappa casein gene increased from mid-pregnancy but at a lower rate and reached approximately 60% by the first day of lactation. Considering the locations and closeness of the casein genes, a non-coordinate expression profile is exhibited by the mouse casein genes, particularly the epsilon casein gene.
authors: Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Trichur, India. sisilamma@yahoo.com
authors: S
authors: S
authors: George
authors: A
authors: A
authors: Springbett
authors: A J
authors: AJ
authors: Clark
authors: A L
authors: AL
authors: Archibald
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002364:Caseins / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression / Q000502:physiology*; D007774:Lactation / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D011247:Pregnancy; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D002364:Caseins; D018076:DNA, Complementary; D012333:RNA, Messenger; D012313:RNA