Molecular and genetic characterization of a non-climacteric phenotype in melon reveals two loci conferring altered ethylene response in fruit.
Fruit ripening and abscission are associated with an ethylene burst in several melon (Cucumis melo) genotypes. In cantaloupe as in other climacteric fruit, exogenous ethylene can prematurely induce abscission, ethylene production, and ripening. Melon genotypes without fruit abscission or without ethylene burst also exist and are, therefore, non-climacteric. In the nonabscising melon fruit PI 161375, exogenous ethylene failed to stimulate abscission, loss of firmness, ethylene production, and expression of all target genes tested. However, the PI 161375 etiolated seedlings displayed the usual ethylene-induced triple response. Genetic analysis on a population of recombinant cantaloupe Charentais x PI 161375 inbred lines in segregation for fruit abscission and ethylene production indicated that both characters are controlled by two independent loci, abscission layer (Al)-3 and Al-4. The non-climacteric phenotype in fruit tissues is attributable to ethylene insensitivity conferred by the recessive allelic forms from PI 161375. Five candidate genes (two ACO, two ACS, and ERS) that were localized on the melon genetic map did not exhibit colocalization with Al-3 or Al-4.
Périn
Christophe
C
Institut National de la Recherche Agronomique, Station de Génétique et d'Amélioration des Fruits et Légumes, Domaine St. Maurice, Boîte Postale 94, 84143 Montfavet cedex, France.
Gomez-Jimenez
MariCarmen
M
Hagen
Lynda
L
Dogimont
Catherine
C
Pech
Jean-Claude
JC
Latché
Alain
A
Pitrat
Michel
M
Lelièvre
Jean-Marc
JM
D000475:Alkenes / Q000378:metabolism / Q000494:pharmacology; D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D000598:Amino Acids, Cyclic / Q000378:metabolism; D002874:Chromosome Mapping; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism; D003433:Crosses, Genetic; D028463:Cucumis melo / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism* / Q000494:pharmacology; D005638:Fruit / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008190:Lyases / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010101:Oxygen Consumption / Q000502:physiology; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics; D018520:Plant Shoots / Q000187:drug effects; D019655:Quantitative Trait, Heritable; D011956:Receptors, Cell Surface / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D013997:Time Factors
D000475:Alkenes; D000598:Amino Acids, Cyclic; C064034:ERS1 protein, S cerevisiae; D005030:Ethylenes; D005656:Fungal Proteins; D008565:Membrane Proteins; D010940:Plant Proteins; D011956:Receptors, Cell Surface; D029701:Saccharomyces cerevisiae Proteins; C083750:ethylene receptors, plant; C023863:1-aminocyclopropane-1-carboxylic acid; C036216:ethylene; C013658:propylene; D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase; D008190:Lyases; C021773:1-aminocyclopropanecarboxylate synthase; D003066:Coenzyme A Ligases; C115795:acyl CoA synthetase 5