title: Rat peripheral CD4+CD8+ T lymphocytes are partially immunocompetent thymus-derived cells that undergo post-thymic maturation to become functionally mature CD4+ T lymphocytes.
abstract: CD4+CD8+ double-positive (DP) T cells represent a minor subpopulation of T lymphocytes found in the periphery of adult rats. In this study, we show that peripheral DP T cells appear among the first T cells that colonize the peripheral lymphoid organs during fetal life, and represent approximately 40% of peripheral T cells during the perinatal period. Later their proportion decreases to reach the low values seen in adulthood. Most DP T cells are small size lymphocytes that do not exhibit an activated phenotype, and their proliferative rate is similar to that of the other peripheral T cell subpopulations. Only 30-40% of DP T cells expresses CD8beta chain, the remaining cells expressing CD8alphaalpha homodimers. However, both DP T cell subsets have an intrathymic origin since they appear in the recent thymic emigrant population after injection of FITC intrathymically. Functionally, although DP T cells are resistant to undergo apoptosis in response to glucocorticoids, they show poor proliferative responses upon CD3/TCR stimulation due to their inability to produce IL-2. A fraction of DP T cells are not actively synthesizing the CD8 coreceptor, and they gradually differentiate to the CD4 cell lineage in reaggregation cultures. Transfer of DP T lymphocytes into thymectomized SCID mice demonstrates that these cells undergo post-thymic maturation in the peripheral lymphoid organs and that their CD4 cell progeny is fully immunocompetent, as judged by its ability to survive and expand in peripheral lymphoid organs, to proliferate in response to CD3 ligation, and to produce IL-2 upon stimulation.
authors: Department of Cell Biology, Faculty of Biology, Complutense University, 28040 Madrid, Spain.
authors: Eva
authors: E
authors: Jiménez
authors: Rosa
authors: R
authors: Sacedón
authors: Angeles
authors: A
authors: Vicente
authors: Carmen
authors: C
authors: Hernández-López
authors: Agustín G
authors: AG
authors: Zapata
authors: Alberto
authors: A
authors: Varas
mesh_terms: D000375:Aging / Q000276:immunology; D000818:Animals; D015704:CD4 Antigens / Q000096:biosynthesis*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D016827:CD8 Antigens / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000276:immunology; D016130:Immunophenotyping; D007275:Injections, Intravenous; D018655:Lymphocyte Count; D051379:Mice; D016513:Mice, SCID; D051381:Rats; D017208:Rats, Wistar; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism* / Q000637:transplantation; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens