Entamoeba histolytica lectins contain unique 6-Cys or 8-Cys chitin-binding domains.
The Jacob lectin, the most abundant glycoprotein in the cyst wall of Entamoeba invadens, contains five unique 6-Cys chitin-binding domains (CBDs). We identified Entamoeba histolytica and Entamoeba dispar genes encoding Jacob homologues, each of which contains two predicted 6-Cys CBDs. A unique 8-Cys CBD was found at the N termini of the E. histolytica chitinase and three other predicted lectins, called Jessie 1 to Jessie 3. The CBDs of four E. histolytica lectins (Jacob, chitinase, and Jessies 2 and 3) were expressed in secretory vesicles of transfected amebae and shown to bind to particulate chitin.
Van Dellen
Katrina
K
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston University School of Dental Medicine, Boston, Massachusetts 02115, USA.
Ghosh
Sudip K
SK
Robbins
Phillips W
PW
Loftus
Brendan
B
Samuelson
John
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D003545:Cysteine / Q000235:genetics* / Q000378:metabolism; D016054:DNA, Protozoan; D004748:Entamoeba histolytica / Q000235:genetics* / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D037102:Lectins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism
D016054:DNA, Protozoan; D006023:Glycoproteins; C457361:Jacob protein, Entamoeba histolytica; D037102:Lectins; D015800:Protozoan Proteins; D002686:Chitin; D002688:Chitinases; D003545:Cysteine