Effects of chlorinated aliphatic hydrocarbons on the fidelity of cell division in human CYP2E1 expressing cells.
Chlorinated organic chemicals are widely used in industry and are present in the environment. Five chlorinated aliphatic hydrocarbons, namely 1-2-dichloroethane, 1,1,2-trichloroethane, trichloroethylene, 2,3-dichlorobutane and 1-chlorohexane were investigated to determine their influence upon the fidelity of cell division in cultured mammalian cells. In order to determine the influence of these chemical compounds upon the fidelity of cell division, a technique known as differential staining of chromosomes and spindle was performed with one genetically engineered cell line and its parental cell line. The genetically engineered cell line used in this study expressed a human P450 enzyme, CYP2E1. Four chemicals, 1-2-dichloroethane, trichloroethylene, 2,3-dichlorobutane and 1-chlorohexane required metabolic bioactivations in order to induce spindle damage in cultured mammalian cells whereas 1,1,2-trichloroethane was a direct-acting spindle poison.
Kim
Adele
A
Samsung Medical Center, Seoul, Korea. aquarius70@hanmail.net
Parry
Elizabeth M
EM
Parry
James M
JM
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002875:Chromosomes / Q000378:metabolism; D006224:Cricetinae; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000737:chemistry / Q000494:pharmacology*; D008941:Spindle Apparatus; D013194:Staining and Labeling / Q000379:methods
D006843:Hydrocarbons, Chlorinated; D019392:Cytochrome P-450 CYP2E1