title: An IS900-like sequence found in a Mycobacterium sp. other than Mycobacterium avium subsp. paratuberculosis.
abstract: The insertion sequence IS900 has been considered specific for Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) and has, therefore, been used as the target gene for diagnostic PCR of M. paratuberculosis. From a healthy dairy cow we have isolated and characterised a mycobacterium harbouring one copy of a sequence with 94% identity to IS900 at the nucleic acid level. The isolate was shown to be related to Mycobacterium cookii, as assessed by 16S rRNA sequencing. Strong amplifications were obtained with several PCR primers described for detection of IS900. This finding shows the need of alternative PCR systems based on other genes than IS900 to confirm the presence of M. paratuberculosis.
authors: Department of Bacteriology, National Veterinary Institute, SE-751 89 Uppsala, Sweden. stina.englund@sva.se
authors: Stina
authors: S
authors: Englund
authors: Göran
authors: G
authors: Bölske
authors: Karl Erik
authors: KE
authors: Johansson
mesh_terms: D000818:Animals; D002417:Cattle; D004251:DNA Transposable Elements / Q000235:genetics*; D009162:Mycobacterium avium / Q000145:classification / Q000235:genetics*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification / Q000235:genetics*; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D012336:RNA, Ribosomal, 16S