title: Alteration of the LRP1B gene region is associated with high grade of urothelial cancer.
abstract: We have delineated regions of interest at chromosome 2q21.2, 2q36.3, and 2q37.1 by deletion mapping of 114 urothelial cancers (UC). Altogether, 17%, 18%, and 63% of the G1, G2, and G3 tumors displayed loss of heterozygosity at chromosome 2q, respectively, The region at 2q21.2 was narrowed down to the LRP1B gene (NT_005129.6). Hemi- and homozygous deletion at the LRP1B gene region was seen in 31 of 114 UCs. Only 8% of the UCs with G1 and none with G2 tumors showed loss of heterozygosity at the LRP1B gene, whereas 49% of the G3 UCs had allelic loss at this region. RT-PCR analysis of the LRP1B gene showed the lack of expression of several exons in 2 of 9 cases analyzed. Our analysis suggests that the LRP1B gene is a candidate tumor suppressor gene in UCs.
authors: Laboratory of Molecular Oncology, Department of Urology, Institute of Human Genetics, Ruprecht-Karls-University, Heidelberg, Germany.
authors: Sigrun
authors: S
authors: Langbein
authors: Orsolya
authors: O
authors: Szakacs
authors: Monica
authors: M
authors: Wilhelm
authors: Farkas
authors: F
authors: Sukosd
authors: Susanne
authors: S
authors: Weber
authors: Anna
authors: A
authors: Jauch
authors: Antonio
authors: A
authors: Lopez Beltran
authors: Peter
authors: P
authors: Alken
authors: Tilman
authors: T
authors: Kälble
authors: Gyula
authors: G
authors: Kovacs
mesh_terms: D002277:Carcinoma / Q000145:classification / Q000235:genetics* / Q000473:pathology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002889:Chromosomes, Human, Pair 2; D044842:Cullin Proteins; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D009693:Nucleic Acid Hybridization; D011973:Receptors, LDL / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000145:classification / Q000235:genetics* / Q000473:pathology; D019459:Urothelium / Q000473:pathology
chemical_list: C503645:CUL3 protein, human; D018797:Cell Cycle Proteins; D044842:Cullin Proteins; D004273:DNA, Neoplasm; C434476:LRP1B protein, human; D011973:Receptors, LDL