title: Introducing antisense oligonucleotides into Pneumocystis carinii.
abstract: To improve the knowledge on Pneumocystis carinii growth, a homologous P. carinii transformation system would provide a tool to promote replication of this fungus. Antisense oligonucleotides have been successfully introduced by electroporation or direct uptake in order to downregulate the prohibitin negative function on cell cycle.
authors: Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Hospices Civils de Lyon, E.A. 3087, Université Claude Bernard Lyon I, 69373, cedex 08, Lyons, France. fdmonb@rockefeller.univ-lyon1.fr
authors: Frédérique
authors: F
authors: de Monbrison
authors: Stéphane
authors: S
authors: Picot
mesh_terms: D000818:Animals; D002453:Cell Cycle; D018274:Electroporation / Q000379:methods*; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism*; D011010:Pneumocystis / Q000254:growth & development* / Q000378:metabolism; D011020:Pneumonia, Pneumocystis / Q000382:microbiology; D000091402:Prohibitins; D011506:Proteins / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins; D014170:Transformation, Genetic
chemical_list: D016376:Oligonucleotides, Antisense; D000091402:Prohibitins; D011506:Proteins; D012097:Repressor Proteins