Expansion of lung V alpha 14 NKT cells by administration of alpha-galactosylceramide-pulsed dendritic cells.
NKT cells, a novel murine lymphoid lineage bearing an invariant T cell receptor encoded by V alpha 14 and J alpha 281 gene segments, recognize a specific ligand glycolipid, alpha-galactosylceramide (alpha-GalCer) in a CD1d-dependent manner. Recent research has revealed that activated V alpha 14 NKT cells have dramatic antitumor effects against a wide variety of tumor cell lines in vivo and in vitro. Here, we demonstrate strong in vivo antitumor effects brought about by treatment with alpha-GalCer-pulsed dendritic cells in comparison with in vitro-activated V alpha 14 NKT cells. Furthermore, we show a significant expansion of endogenous V alpha 14 NKT cells in the lung following the administration of alpha-GalCer-pulsed dendritic cells. The feasibility of immunotherapy with alpha-GalCer-pulsed dendritic cells is discussed.
Akutsu
Yasunori
Y
CREST (Core Research for Evolutional Science and Technology) Project, JST, Kawaguchi, Saitama 332-0012, Japan.
Nakayama
Toshinori
T
Harada
Michishige
M
Kawano
Tetsu
T
Motohashi
Shinichiro
S
Shimizu
Eiko
E
Ito
Toshihiro
T
Kamada
Noriaki
N
Saito
Takeshi
T
Matsubara
Hisahiro
H
Miyazawa
Yukimasa
Y
Ochiai
Takenori
T
Taniguchi
Masaru
M
D000818:Animals; D018949:Antigens, CD1 / Q000096:biosynthesis; D055610:Antigens, CD1d; D000970:Antineoplastic Agents / Q000494:pharmacology; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D019070:Cell Lineage; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003713:Dendritic Cells / Q000166:cytology*; D005699:Galactosylceramides / Q000378:metabolism* / Q000494:pharmacology; D007167:Immunotherapy / Q000379:methods*; D007963:Leukocytes, Mononuclear; D008024:Ligands; D008168:Lung / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009362:Neoplasm Metastasis; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018949:Antigens, CD1; D055610:Antigens, CD1d; D000970:Antineoplastic Agents; D002860:Chromium Radioisotopes; D005699:Galactosylceramides; D008024:Ligands; C493154:alpha-galactosylceramide; D012313:RNA