title: [Expression of integrin alpha 6 in hepatic sinusoidal capillaration].
abstract: OBJECTIVE
  To explore the expression of integrin alpha 6 in hepatic sinusoidal capillaration.
  
  
  METHODS
  The rat hepatic fibrosis model was established by injection of carbon tetrachloride subcutaneously. Then the expression of laminin and integrin alpha 6 subunit was observed by immunohistochemistry and dot immuno-blotting.
  
  
  RESULTS
  We observed sinusoidal capillaration formed by deposition of laminin along sinusoids in Disse interspace by immunohistochemistry staining. In normal rat the expression of integrin alpha 6 was restricted to portal vascular endothelial cells and bile duct epithelial cell membranes. No expression was observed in sinusoidal endothelial cell membranes. When capillaration integrin alpha 6 was detected in a continuous pattern along the sinusoids, the content of integrin alpha 6 was significantly higher in fibrotic liver tissues than in normal liver tissues as measured by dot immuno-blotting (P<0.05).
  
  
  CONCLUSIONS
  During fibrogenesis, laminin continuously accumulate in liver tissues and form basement membrane resulting in sinusoidal capillaration, and then induce the expression of integrin alpha 6 on SEC membranes.
authors: Digestive Medicine Institute of Huaxi Hospital of Sichuan University, Chengdu 610041, China.
authors: Wenjun
authors: W
authors: Xiao
authors: Yiping
authors: Y
authors: Wang
authors: Xiaojing
authors: X
authors: Liu
authors: Minghui
authors: M
authors: Huang
authors: Tao
authors: T
authors: Gan
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis*; D002251:Carbon Tetrachloride; D015151:Immunoblotting; D007150:Immunohistochemistry; D039503:Integrin alpha6; D007797:Laminin / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D015703:Antigens, CD; D039503:Integrin alpha6; D007797:Laminin; D002251:Carbon Tetrachloride