Identification of in vivo expressed vaccine candidate antigens from Staphylococcus aureus.
For the design of potent subunit vaccines, it is of paramount importance to identify all antigens immunologically recognized by a patient population infected with a pathogen. We have developed a rapid and efficient procedure to identify such commonly recognized antigens, and here we provide a comprehensive in vivo antigenic profile of Staphylococcus aureus, an important human pathogen. S. aureus peptides were displayed on the surface of Escherichia coli via fusion to one of two outer membrane proteins (LamB and FhuA) and probed with sera selected for high Ab titer and opsonic activity. A total of 60 antigenic proteins were identified, most of which are located or predicted to be located on the surface of the bacterium or secreted. The identification of these antigens and their reactivity with individual sera from patients and healthy individuals greatly facilitate the selection of promising vaccine candidates for further evaluation. This approach, which makes use of whole genome sequence information, has the potential to greatly accelerate and facilitate the formulation of novel vaccines and is applicable to any pathogen that induces Abs in humans and/or experimental animals.
Etz
Hildegard
H
InterCell Biomedizinische Forschungs- und Entwicklungs-AG, Campus Vienna Biocenter 6, 1030 Vienna, Austria.
Minh
Duc Bui
DB
Henics
Tamás
T
Dryla
Agnieszka
A
Winkler
Birgit
B
Triska
Christine
C
Boyd
Aoife P
AP
Söllner
Johannes
J
Schmidt
Walter
W
von Ahsen
Uwe
U
Buschle
Michael
M
Gill
Steven R
SR
Kolonay
James
J
Khalak
Hanif
H
Fraser
Claire M
CM
von Gabain
Alexander
A
Nagy
Eszter
E
Meinke
Andreas
A
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D004269:DNA, Bacterial; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D016680:Genome, Bacterial; D015698:Genomic Library; D006801:Humans; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010587:Phagocytosis; D018272:Porins; D011991:Receptors, Virus / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013203:Staphylococcal Infections / Q000097:blood / Q000276:immunology*; D013209:Staphylococcal Vaccines / Q000235:genetics / Q000276:immunology*; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D018985:Epitopes, B-Lymphocyte; D029968:Escherichia coli Proteins; C050900:FhuA protein, E coli; D018272:Porins; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D013209:Staphylococcal Vaccines; D014614:Vaccines, Synthetic; C016532:maltoporins