[Radioadaptive response in vitro of unstimulated rat lymphocytes according to metallothionein test].
It was shown that 24 h after acute action of gamma-radiation in vitro on unstimulated rat lymphocytes the metallothionein (MT) level did not change for doses of 0.01; 0.5 and 1.0 Gy, while dosed of 1.75 and 2.5 Gy increased this parameter 2 and 2.6 times on the average. After exposure to 0.01 Gy of adaptive radiation 2 h before exposure to 1.75 Gy of the damaging dose reliably lower (1.5 times on the average) increase in MT content was found. The heterogeneity in display of the radioadaptive response by MT test for various lymphocyte preparations was found. Two possible mechanisms of the obtained changes were discussed. One of them is connected with the selection irradiation death of cells with the initially low MT level, and the other is caused by postradiation MT synthesis as a result of accumulation of the DNA damages and MT genes amplification.
Koterov
A N
AN
State Research Center-Institute of Biophysics, Russian Ministry of Health, Moscow, 123182 Russia.
Filippovich
I V
IV
D000818:Animals; D016923:Cell Death; D004260:DNA Repair; D005784:Gene Amplification; D008214:Lymphocytes / Q000528:radiation effects*; D008668:Metallothionein / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D011829:Radiation Dosage; D011836:Radiation Tolerance; D051381:Rats; D013997:Time Factors
D008668:Metallothionein