[Expression of thermal stable, soluble hepatitis E virus recombinant antigen].
BACKGROUND
To obtain thermal stable, soluble, biologically active hepatitis E virus recombinant antigen using thioredoxin fusion expression system.


METHODS
HEV ORF2 gene fragment (6964-7126 nt) was inserted into thioredoxin fusion expression vector pThioHisA. The recombinant plasmid was transformed into E. coli BL21 strain. After induction with IPTG, cells were lysed and the supernatant was subjected to 80 degree treatment for 10 minutes. After centrifugation, the supernatant was tested by ELISA.


RESULTS
SDS-PAGE analysis showed the thioredoxin. HEV fusion protein was highly expressed and was thermally stable, soluble. HEV specific ELISA confirmed this fusion protein possessing HEV specific antigenicity.


CONCLUSIONS
Using thioredoxin fusion expression system, a soluble, thermal stable, biologically active HEV recombinant antigen was successfully expressed.
Zhang
Mingcheng
M
Hepatitis Branch, Institute of Virology,Chinese Academy of Preventive Medicine, Beijing 100052, China.
Yi
Yao
Y
Zhan
Meiyun
M
Liu
Chongbai
C
Bi
Shengli
S
D000956:Antigens, Viral / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D016752:Hepatitis E virus / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D013879:Thioredoxins / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
D000956:Antigens, Viral; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D013879:Thioredoxins