Dissecting tumour pathophysiology using intravital microscopy.
For a systemically administered therapeutic agent to reach neoplastic cells, it must enter the blood circulation, cross the vessel wall, move through the extracellular matrix and avoid getting cleared by the lymphatics. In tumours, each of these barriers is abnormal, changes with space and time, and depends on host-tumour interactions. Intravital microscopy has provided unprecedented molecular, cellular, anatomical and functional insights into these barriers and has revealed new approaches to improved detection and treatment.
Jain
Rakesh K
RK
Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston 02114, USA. jain@steele.mgh.harvard.edu
Munn
Lance L
LL
Fukumura
Dai
D
D000818:Animals; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins; D006168:Guinea Pigs; D006801:Humans; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000503:physiopathology* / Q000648:ultrastructure*; D009389:Neovascularization, Pathologic; D011817:Rabbits; D051381:Rats; D013997:Time Factors; D014407:Tumor Cells, Cultured
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins