Development and application of a new scheme for typing Campylobacter jejuni and Campylobacter coli by PCR-based restriction fragment length polymorphism analysis.
A molecular typing approach for Campylobacter jejuni and Campylobacter coli was developed with restriction fragment length polymorphism analysis of a 9.6-kb PCR-amplified portion of the lipopolysaccharide gene cluster. Sixty-one Penner serotype reference strains were analyzed with this new genotyping scheme, and 32 genogroups were found. Eleven additional genogroups were obtained from 87 clinical C. jejuni strains tested. This molecular typing method shows a correlation with the Penner heat-stable serotyping method, a phenotypic typing method based on lipopolysaccharide structures that is often used as a "gold standard" for subtyping Campylobacter spp. This strong correlation suggests that the data obtained can be directly compared with epidemiological data collected in the past by classical serotyping of C. jejuni and C. coli. In contrast to the high percentage of nontypeability by phenotyping, this molecular typing method results in 100% typeability and provides a superior alternative to serotyping.
Shi
Feng
F
Department of Biotechnology and Environmental Biology, Royal Melbourne Institute of Technology University, Bundoora West Campus, Bldg. 223 Level 1, Plenty Road, Bundoora, Victoria 3083, Australia. shi.feng@rmit.edu.au
Chen
Yuen Yuen
YY
Wassenaar
Trudy M
TM
Woods
Walter H
WH
Coloe
Peter J
PJ
Fry
Benjamin N
BN
D000818:Animals; D001483:Base Sequence; D002169:Campylobacter Infections / Q000175:diagnosis / Q000662:veterinary; D017000:Campylobacter coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011200:Poultry; D011201:Poultry Diseases / Q000382:microbiology; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping / Q000379:methods
D017931:DNA Primers; D008070:Lipopolysaccharides