Nutritional quality of extruded kidney bean (Phaseolus vulgaris L. var. Pinto) and its effects on growth and skeletal muscle nitrogen fractions in rats.
The influence of extrusion cooking on the protein content, amino acid profile, and concentration of antinutritive compounds (phytic acid, condensed tannins, polyphenols, trypsin, chymotrypsin, alpha-amylase inhibitors, and hemagglutinating activity) in kidney bean seeds (Phaseolus vulgaris L. var. Pinto) was investigated. Growing male rats were fed diets based on casein containing raw or extruded kidney beans with or without methionine supplementation for 8 or 15 d. Rates of growth, food intake, and protein efficiency ratio were measured and the weight of the gastrocnemius muscle and the composition of its nitrogenous fraction was determined. Extrusion cooking reduced (P < 0.01) phytic acid, condensed tannins, and trypsin, chymotrypsin, and (alpha-amylase inhibitory activities. Furthermore, hemagglutinating activity was abolished by extrusion treatment. Protein content was not affected by this thermal treatment. Rats fed raw kidney bean lost BW rapidly and the majority died by 9 d. Pretreatment of the beans by extrusion cooking improved food intake and utilization by the rats and they gained BW. Supplementation of extruded kidney bean with methionine further enhanced (P < 0.01) food conversion efficiency and growth. However, BW gains and muscle composition still differed (P < 0.01) from those of rats fed a high-quality protein.
Marzo
F
F
Laboratory of Animal Physiology and Nutrition, School of Agronomy, Universidad Pública de Navarra, Pamplona, Spain. marzo@unavarra.es
Alonso
R
R
Urdaneta
E
E
Arricibita
F J
FJ
Ibáñez
F
F
D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000592:standards; D002149:Energy Intake; D005511:Food Handling / Q000379:methods*; D006384:Hemagglutination; D008297:Male; D008715:Methionine / Q000008:administration & dosage; D018482:Muscle, Skeletal / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D009753:Nutritive Value; D027805:Phaseolus / Q000737:chemistry*; D010833:Phytic Acid / Q000032:analysis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D013634:Tannins / Q000032:analysis; D014361:Trypsin Inhibitors / Q000032:analysis; D015430:Weight Gain / Q000502:physiology*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D004044:Dietary Proteins; D013634:Tannins; D014361:Trypsin Inhibitors; D010833:Phytic Acid; D008715:Methionine; D000516:alpha-Amylases