Evaluation of N-acetylchitooligosaccharides as the main carbon sources for the growth of intestinal bacteria.
N-Acetylchitooligosaccharides ((GlcNAc)(n)) with different degrees of polymerization (n=1-6) were prepared as the main carbon sources in media for evaluating the growth of nine intestinal bacteria. A chitohydrolysate was prepared by hydrolyzing shrimp-shell chitin using HCl. After purification, the purity of each (GlcNAc)(1-6) was >86%. The growth of intestinal bacteria was carried out in a basal medium (BM) containing 0.2% (w/v) of each sugar or glucose as the main carbon source and was evaluated using maximum cell densities and specific growth rates. Bacteroides fragilis and Clostridium perfringens could respectively utilize GlcNAc and (GlcNAc)(2) more efficiently for growth than glucose. Bifidobacterium adolescentis and Eubacterium limosum could use (GlcNAc)(1-6) slightly as their main carbon source. Escherichia coli, Lactococcus lactis and Proteus vulgaris could utilize glucose more efficiently than (GlcNac)(1-6). GlcNAc was used more readily than (GlcNAc)(2-6) by Staphylococcus aureus, exhibiting almost the same specific growth rates. In BM, Streptococcus faecalis grew well even without adding each of the sugars tested.
Chen
Hsing-Chen
HC
Department of Food Science, National Taiwan Ocean University, Keelung 202, Taiwan. hcchen@mail.ntou.edu.tw
Chang
Chiao-Ching
CC
Mau
Wei-Juin
WJ
Yen
Lin-Shiun
LS
D000117:Acetylglucosamine / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D001704:Biopolymers; D050260:Carbohydrate Metabolism; D002244:Carbon / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology*; D009844:Oligosaccharides / Q000378:metabolism*; D013045:Species Specificity
D001704:Biopolymers; D003470:Culture Media; D009844:Oligosaccharides; D002686:Chitin; D002244:Carbon; D005947:Glucose; D000117:Acetylglucosamine