Analysis of homozygous deletion of the p16 gene and correlation with survival in patients with glioblastoma multiforme.
OBJECTIVE
One of the most frequent genetic abnormalities found in patients with glioblastoma multiforme (GBM) is homozygous deletion of the p16 tumor suppressor gene. The authors investigated whether this deletion is associated with prognosis in patients with GBM.


METHODS
In 46 adult patients with supratentorial GBM, homozygous deletion of the p16 gene in tumor DNA was examined using the multiplex polymerase chain reaction assay. The deletion was confirmed in 14 (30.4%) of 46 patients, eight (30.8%) of 26 men and six (30.0%) of 20 women. Cox proportional hazard regression analysis, adjusted for age at surgery, the Karnofsky Performance Scale score, extent of resection, and the MIB-1 labeling index. revealed that homozygous deletion of the p16 gene was significantly associated with overall survival and progression-free survival in men, but not in women.


CONCLUSIONS
The results of this study suggest that p16 homozygous deletion is a significant unfavorable prognostic factor in male patients with GBM.
Kamiryo
Takanori
T
Department of Neurosurgery, Kumamoto University Medical School, Japan. tkamiryo-nsu@umin.ac.jp
Tada
Kenji
K
Shiraishi
Shoji
S
Shinojima
Naoki
N
Nakamura
Hideo
H
Kochi
Masato
M
Kuratsu
Jun-ichi
J
Saya
Hideyuki
H
Ushio
Yukitaka
Y
D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000601:surgery; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D018572:Disease-Free Survival; D005260:Female; D017353:Gene Deletion; D005909:Glioblastoma / Q000453:epidemiology / Q000235:genetics* / Q000601:surgery; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011379:Prognosis; D015996:Survival Rate
D019941:Cyclin-Dependent Kinase Inhibitor p16