A simple method for enrichment of uninucleate conidia of Aspergillus oryzae.
Aspergillus oryzae produces multinucleate conidia, which makes the obtaining of homokaryons labor-intensive. Analysis of conidia by flow cytometry clarified the relationship that conidia of lower nuclear number were smaller in size. Based on this, we have developed a simple way to enrich uninucleate conidia with a membrane filter. Our results also suggest that the method is useful for elimination of heterokaryons.
Hara
Seiichi
S
Noda Institute for Scientific Research, Noda-shi, Chiba, Japan. shara@mail.kikkoman.co.jp
Tsuji
Ryohei F
RF
Hatamoto
Osamu
O
Masuda
Tsutomu
T
D001236:Aspergillus oryzae / Q000254:growth & development* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D005434:Flow Cytometry; D008863:Microspheres; D012542:Scattering, Radiation; D014462:Ultrafiltration