title: Involvement of membrane signaling in the bystander effect in irradiated cells.
abstract: We have shown previously that when confluent cultures of mammalian cells are exposed to very low fluences of alpha particles, fluences whereby only 1-3% of the cell nuclei are traversed by a particle, genetic effects, including specific gene mutations and sister chromatid exchanges, are induced in neighboring, nonirradiated ("bystander") cells (H. Nagasawa and J. B. Little, Cancer Res., 52: 6394-6396, 1992; H. Nagasawa and J. B. Little, Radiat. Res., 152: 552-557, 1999). The present experiments were designed to determine whether signaling pathways arising in the cell membrane may mediate this effect. Cells were irradiated in the presence of Filipin, an agent that disrupts lipid rafts, effectively inhibiting membrane signaling, and the induction of sister chromatid exchange and HPRT mutations by very low fluences of alpha particles (mean doses 0.17-0.5 cGy) was measured. Filipin completely suppressed the induction of both genetic effects in bystander cells. After exposure to 10 cGy, when most mutations occurred in directly irradiated cells, no suppressive effect of Filipin was observed. These results suggest that membrane signaling may play an important role in the bystander effect of radiation. On the other hand, the effects in directly irradiated cells do not appear to be mediated via the cell membrane.
authors: Laboratory of Radiobiology, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
authors: Hatsumi
authors: H
authors: Nagasawa
authors: Aida
authors: A
authors: Cremesti
authors: Richard
authors: R
authors: Kolesnick
authors: Zvi
authors: Z
authors: Fuks
authors: John B
authors: JB
authors: Little
mesh_terms: D000512:Alpha Particles; D000818:Animals; D024201:Bystander Effect / Q000502:physiology*; D016466:CHO Cells / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D006224:Cricetinae; D005372:Filipin / Q000494:pharmacology; D006028:Glycosphingolipids / Q000378:metabolism / Q000502:physiology; D009154:Mutation; D010430:Pentosyltransferases / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000528:radiation effects
chemical_list: D006028:Glycosphingolipids; D005372:Filipin; D010430:Pentosyltransferases; C083867:hypoxanthine-guanine-xanthine phosphoribosyltransferase