title: Developmental expression of trehalase: role of transcriptional activation.
abstract: The third postnatal week of mouse development is characterized by dramatic changes of gene expression in the small intestine. Although these changes are often assumed to reflect regulation at the level of transcription, to date there have been no direct investigations of this. In the current study we have used trehalase as a marker of intestinal maturation. Highly sensitive reverse transcriptase-polymerase chain reaction methods were developed for semi-quantitative analysis of both initial and mature transcripts, i.e., hnRNA and mRNA. Jejunums collected during normal development (specifically from postnatal days 8-21) showed parallel increases in the levels of trehalase hnRNA and mRNA. Likewise, when precocious gut maturation was elicited by dexamethasone administration on days 8-10, both initial and mature trehalase transcripts were significantly increased, although with a relatively slow time course. We conclude that both normal and glucocorticoid-induced maturation of trehalase expression reflect transcriptional activation. However, the slow time course of the glucocorticoid effect suggests that trehalase may not be a primary response gene.
authors: Department of Pediatrics, Baylor College of Medicine, One Baylor College of Medicine, Houston, TX 77030, USA.
authors: Hans
authors: H
authors: Gartner
authors: Pramila
authors: P
authors: Shukla
authors: Diane C
authors: DC
authors: Markesich
authors: Nitikul S
authors: NS
authors: Solomon
authors: Thomas J
authors: TJ
authors: Oesterreicher
authors: Susan J
authors: SJ
authors: Henning
mesh_terms: D000367:Age Factors; D000818:Animals; D000833:Animals, Suckling; D003907:Dexamethasone / Q000008:administration & dosage; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007421:Intestine, Small / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012313:RNA / Q000032:analysis / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation / Q000187:drug effects; D014198:Trehalase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012313:RNA; D003907:Dexamethasone; D014198:Trehalase