title: Desumoylation activity of Axam, a novel Axin-binding protein, is involved in downregulation of beta-catenin.
abstract: Axam has been identified as a novel Axin-binding protein that inhibits the Wnt signaling pathway. We studied the molecular mechanism by which Axam stimulates the downregulation of beta-catenin. The C-terminal region of Axam has an amino acid sequence similar to that of the catalytic region of SENP1, a SUMO-specific protease (desumoylation enzyme). Indeed, Axam exhibited activity to remove SUMO from sumoylated proteins in vitro and in intact cells. The Axin-binding domain is located in the central region of Axam, which is different from the catalytic domain. Neither the Axin-binding domain nor the catalytic domain alone was sufficient for the downregulation of beta-catenin. An Axam fragment which contains both domains was able to decrease the level of beta-catenin. On substitution of Ser for Cys(547) in the catalytic domain, Axam lost its desumoylation activity. Further, this Axam mutant decreased the activity to downregulate beta-catenin. Although Axam strongly inhibited axis formation and expression of siamois, a Wnt-response gene, in Xenopus embryos, Axam(C547S) showed weak activities. These results demonstrate that Axam functions as a desumoylation enzyme to downregulate beta-catenin and suggest that sumoylation is involved in the regulation of the Wnt signaling pathway.
authors: Department of Biochemistry, Faculty of Medicine, Hiroshima University, Minami-ku, Hiroshima 734-8551, Japan.
authors: Takayuki
authors: T
authors: Kadoya
authors: Hideki
authors: H
authors: Yamamoto
authors: Toshiaki
authors: T
authors: Suzuki
authors: Akira
authors: A
authors: Yukita
authors: Akimasa
authors: A
authors: Fukui
authors: Tatsuo
authors: T
authors: Michiue
authors: Toshimasa
authors: T
authors: Asahara
authors: Keiji
authors: K
authors: Tanaka
authors: Makoto
authors: M
authors: Asashima
authors: Akira
authors: A
authors: Kikuchi
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D060466:Axin Protein; D001483:Base Sequence; D001665:Binding Sites; D019521:Body Patterning / Q000235:genetics; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D015536:Down-Regulation; D006801:Humans; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins; D025842:SUMO-1 Protein / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D015534:Trans-Activators; D051153:Wnt Proteins; D014981:Xenopus / Q000196:embryology / Q000235:genetics; D029867:Xenopus Proteins; D029961:Zebrafish Proteins; D051176:beta Catenin
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D060466:Axin Protein; C495266:CTNNB1 protein, Xenopus; C495270:CTNNB1 protein, human; D002352:Carrier Proteins; C495268:Ctnnb1 protein, rat; D003598:Cytoskeletal Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D025842:SUMO-1 Protein; C417511:Senp2 protein, rat; D015534:Trans-Activators; D051153:Wnt Proteins; D029867:Xenopus Proteins; D029961:Zebrafish Proteins; C555454:axin1 protein, Xenopus; D051176:beta Catenin; D004247:DNA