title: Comparative genome sequencing for discovery of novel polymorphisms in Bacillus anthracis.
abstract: Comparison of the whole-genome sequence of Bacillus anthracis isolated from a victim of a recent bioterrorist anthrax attack with a reference reveals 60 new markers that include single nucleotide polymorphisms (SNPs), inserted or deleted sequences, and tandem repeats. Genome comparison detected four high-quality SNPs between the two sequenced B. anthracis chromosomes and seven differences among different preparations of the reference genome. These markers have been tested on a collection of anthrax isolates and were found to divide these samples into distinct families. These results demonstrate that genome-based analysis of microbial pathogens will provide a powerful new tool for investigation of infectious disease outbreaks.
authors: The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA., Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA.
authors: Timothy D
authors: TD
authors: Read
authors: Steven L
authors: SL
authors: Salzberg
authors: Mihai
authors: M
authors: Pop
authors: Martin
authors: M
authors: Shumway
authors: Lowell
authors: L
authors: Umayam
authors: Lingxia
authors: L
authors: Jiang
authors: Erik
authors: E
authors: Holtzapple
authors: Joseph D
authors: JD
authors: Busch
authors: Kimothy L
authors: KL
authors: Smith
authors: James M
authors: JM
authors: Schupp
authors: Daniel
authors: D
authors: Solomon
authors: Paul
authors: P
authors: Keim
authors: Claire M
authors: CM
authors: Fraser
mesh_terms: D000818:Animals; D000881:Anthrax / Q000382:microbiology; D001408:Bacillus anthracis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D023482:Bioterrorism; D007446:Chromosome Inversion; D019295:Computational Biology; D004196:Disease Outbreaks; D005819:Genetic Markers; D014644:Genetic Variation; D016680:Genome, Bacterial; D023281:Genomics; D006801:Humans; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010802:Phylogeny; D010957:Plasmids; D020641:Polymorphism, Single Nucleotide; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D013045:Species Specificity; D019895:Transposases / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: D005819:Genetic Markers; D019895:Transposases