Muscle strength following direct injection of doxorubicin into rabbit sternocleidomastoid muscle in situ.
Direct intramuscular injection of doxorubicin results in permanent myofiber loss. A previous phase I trial demonstrated that such injections could successfully treat blepharospasm and hemifacial spasm. Our previous in vitro study demonstrated that doxorubicin resulted in a dose-dependent reduction in isometric force generation in sternocleidomastoid muscle in rabbits. This present study examined alterations in force generation in these treated muscles in situ, i.e., with the blood and nerve supply intact. Two months after a single doxorubicin injection, functional changes in peak twitch, tetanic force generation, and fatigue rate were assessed in control and doxorubicin-treated sternocleidomastoid muscles in rabbits. Peak force measurements were reduced in the treated muscles. These reductions in muscle strength were significantly greater at tetanic peak amplitudes. Fatigue rate was not altered by doxorubicin treatment of the sternocleidomastoid muscles. These findings support the potential clinical use of doxorubicin chemomyectomy for the treatment of patients with cervical dystonia.
Falkenberg
Jon H
JH
Department of Physiology, University of Minnesota, Minneapolis, Minnesota, USA.
Iaizzo
Paul A
PA
Department of Physiology, University of Minnesota, Minneapolis, Minnesota, USA.|Department of Anesthesiology, University of Minnesota, Minneapolis, Minnesota, USA.
McLoon
Linda K
LK
Department of Ophthalmology, University of Minnesota, Room 374 LRB, 2001 6th Street SE, Minneapolis, Minnesota 55455, USA.|Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.
D000818:Animals; D004317:Doxorubicin / Q000494:pharmacology*; D007273:Injections, Intramuscular; D009119:Muscle Contraction; D018763:Muscle Fatigue / Q000187:drug effects / Q000502:physiology; D009334:Neck Muscles / Q000187:drug effects* / Q000502:physiology*; D011817:Rabbits; D012016:Reference Values
D004317:Doxorubicin