Removal of endotoxin in blood by polymyxin B immobilized polystyrene-derivative fiber.
Polymyxin B, a cationic cyclic decapeptide antibiotic, is well known to bind endotoxin and to neutralize its toxicity. Based on this principle, polymyxin B was immobilized on the chloroacetamidomethylated polystyrene fiber that is reinforced by polypropylene. The adsorbing capacity of the obtained fibers (polymyxin B immobilized fiber [PMX-F]) was evaluated on endotoxin and other serum components in serum and on heparin in phosphate-buffered saline. Fluorescein isothiocyanate-labeled or tetramethylrhodamine isothiocyanate-labeled lipopolysaccharide (LPS) was used as endotoxin. The measurement of the fluorescence intensity showed that PMX-F adsorbed these LPSs depending on their concentration and on amount. The adsorption of endotoxin was confirmed by desorption of LPS from PMX-F as well. PMX-F adsorbed serum amyloid protein A besides LPS, but neither C-reactive protein nor low-density lipoprotein. The adsorbing property of heparin was low.
Teramoto
Kazuo
K
Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan.
Nakamoto
Yoshiaki
Y
Kunitomo
Tetsunosuke
T
Shoji
Hisataka
H
Tani
Toru
T
Hanazawa
Kazuyoshi
K
Kodama
Masashi
M
D000042:Absorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001781:Blood Component Removal; D002417:Cattle; D004731:Endotoxins / Q000097:blood*; D019793:Fluorescein; D006801:Humans; D011112:Polymyxin B / Q000494:pharmacology*; D011126:Polypropylenes; D011137:Polystyrenes; D012235:Rhodamines
D000900:Anti-Bacterial Agents; D004731:Endotoxins; D011126:Polypropylenes; D011137:Polystyrenes; D012235:Rhodamines; C005358:tetramethylrhodamine; D011112:Polymyxin B; D019793:Fluorescein