(T2AG3)n telomeric sequence hybridization indicating centric fusion rearrangements in the karyotype of the rodent oryzomys subflavus.
Chromosome preparations of 30 specimens of Oryzomys subflavus trapped in eight Brazilian localities were C-, and G-banded and analyzed by fluorescence in situ hybridization (FISH). Two karyotypes were found, 2n = 50/FN = 64, at three coastal localities of the Atlantic Forest domain, and 2n = 58/FN = 70 at two sites located in the Cerrado biome, Brazil Central. Two fluorescence in situ hybridization (FISH) patterns of the telomeric sequence (T2AG3)n were observed: in both karyotypes the probes hybridized to the telomeres of all chromosomes and also a hybridization signal in the centromeric regions of two autosome pairs was seen in the 2n = 50 karyotype. These results, together with the occurrence of other diploid numbers described in the literature, suggest that 0. subflavus is a complex species, bearing fusion/fission rearrangements proper to the different biomes which it inhabits.
Andrades-Miranda
Jaqueline
J
Departamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Zanchin
Nilson I T
NI
Oliveira
Luiz F B
LF
Langguth
Alfredo R
AR
Mattevi
Margarete S
MS
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D001938:Brazil; D002503:Centromere / Q000235:genetics; D002871:Chromosome Banding; D002875:Chromosomes; D004171:Diploidy; D005843:Geography; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008938:Mitosis; D011995:Recombination, Genetic; D016205:Sigmodontinae / Q000235:genetics*; D016615:Telomere / Q000235:genetics*