Indirect high-performance liquid chromatographic separation of stereoisomers of beta-alkyl-substituted amino acids by the application of (S)-N-(4-nitrophenoxycarbonyl)phenylalanine methoxyethyl ester as chiral derivatizing agent.
The indirect high-performance liquid chromatographic enantioresolution of beta-alkyl-substituted analogues of tyrosine, phenylalanine, 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid and tryptophan is reported. (S)-N-(4-Nitrophenoxycarbonyl)phenylalanine methoxyethyl ester, a recently developed chiral derivatizing agent, was used for pre-column derivatization of the investigated analytes. The diastereoisomers formed were analysed under reversed-phase conditions. The effects of parameters such as the amount and type of the organic modifier and the type of the stationary phase on the resolution and retention of the derivatives were investigated. Chromatographic conditions were found for the separation of all four stereoisomers of each analyte.
Vékes
Erika
E
Department of Inorganic and Analytical Chemistry, University of Szeged, Hungary.
Török
Gabriella
G
Péter
Antal
A
Sapi
János
J
Tourwé
Dirk
D
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004952:Esters / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry*; D009574:Nitro Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D000596:Amino Acids; D004952:Esters; D007202:Indicators and Reagents; C467080:N-(4-nitrophenoxycarbonyl)phenylalanine methoxyethyl ester; D009574:Nitro Compounds