Effects of chitin/chitosan and their oligomers/monomers on migrations of fibroblasts and vascular endothelium.
Effects of chitin/chitosan and their oligomers/monomers on migrations of fibroblasts (3T6) and vascular endothelial cells (human umbilical vascular endothelial cell: HUVEC) were evaluated in vitro. In direct migratory assay using the blind well chamber method, migratory activity of 3T6 was seen to be reduced by chitin, chitosan and the chitosan monomer (GlcN). Migratory activity of HUVECs was enhanced by chitin, chitosan and the chitin monomer (GlcNAc), and was reduced by chitosan oligomers and GlcN. Supernatant of 3T6 preincubated with chitin or chitosan reduced migratory activity of 3T6 cells. Supernatant of HUVECs preincubated with chitosan also reduced migratory activity of HUVECs, but supernatant preincubated with chitin had no effect on them. In a proliferation (MTT reduction) assay, none of the samples affected proliferation of either type of cell.
Okamoto
Yoshiharu
Y
Department of Veterinary Surgery, Faculty of Agriculture, Tottori University, Japan. yokamoto@muses.tottori-u.ac.jp
Watanabe
Midori
M
Miyatake
Katsuyuki
K
Morimoto
Minoru
M
Shigemasa
Yoshihiro
Y
Minami
Saburo
S
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D048271:Chitosan; D004396:Coloring Agents / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D051379:Mice; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology
D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D002686:Chitin; D048271:Chitosan; C022616:thiazolyl blue