title: A 4-AP-sensitive current is enhanced by chronic carbon monoxide exposure in coronary artery myocytes.
abstract: A physiological role of carbon monoxide has been suggested for coronary myocytes; however, direct evidence is lacking. The objective of this study was to test the effect of chronic carbon monoxide exposure on the K(+) currents of the coronary myocytes. The effect of 3-wk chronic exposure to carbon monoxide was assessed on K(+) currents in isolated rat left coronary myocytes by the use of the patch-clamp technique in the whole cell configuration. Moreover, membrane potential studies were performed on coronary artery rings using intracellular microelectrodes, and coronary blood flow in isolated heart preparation was recorded. Carbon monoxide did not change the amplitude of global whole cell K(+) current, but it did increase the component sensitive to 1 mM 4-aminopyridine. Carbon monoxide exposure hyperpolarized coronary artery segments by approximately 10 mV and, therefore, increased their sensitivity to 4-aminopyridine. This effect was associated with an enhancement of coronary blood flow. We conclude that chronic carbon monoxide increases a 4-aminopyridine-sensitive current in isolated coronary myocytes. This mechanism could, in part, contribute to hyperpolarization and to increased coronary blood flow observed with carbon monoxide.
authors: Unité de Préconditionnement du Myocarde, Unité de Formation et de Recherche Sciences, 49045 Angers Cedex, France.
authors: Christine
authors: C
authors: Barbé
authors: Eric
authors: E
authors: Dubuis
authors: Annie
authors: A
authors: Rochetaing
authors: Paul
authors: P
authors: Kreher
authors: Pierre
authors: P
authors: Bonnet
authors: Christophe
authors: C
authors: Vandier
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology*; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D002248:Carbon Monoxide / Q000008:administration & dosage*; D003326:Coronary Circulation; D003331:Coronary Vessels / Q000187:drug effects* / Q000502:physiology*; D004553:Electric Conductivity; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D018408:Patch-Clamp Techniques; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D015221:Potassium Channels / Q000187:drug effects* / Q000502:physiology; D051381:Rats
chemical_list: D026902:Potassium Channel Blockers; D015221:Potassium Channels; D002248:Carbon Monoxide; D015761:4-Aminopyridine