Analysis of heat-shock related gene expression in head-and-neck cancer using cDNA arrays.
OBJECTIVE
The aim of the present study was to identify genes regulated in the early response to heat-shock in human head-and-neck cancer cells using a cDNA array.


METHODS
IMC-3 cells were heat-shocked at 44 degrees C for 30 min, then incubated for 6 h. After 6 h incubation, mRNAs were extracted. Early gene expressions in IMC-3 cells were analyzed using a cDNA array after heat-shock. In addition, reverse transcription-polymerase chain reaction (RT-PCR) was performed to identify the expressions of mRNA for confirming features by cDNA array analysis in several other cell lines (KB, T3M-1, SCC-TF) as well as in IMC-3 cells.


RESULTS
Twenty-eight genes were found to be induced or upregulated by heat-shock in IMC-3 cells. These included genes involved in the apoptosis (e.g., CC3, caspase10), tumor invasion (e.g., CC3, TIMP-3), cell cycle checkpoint control (e.g., DP-1, CDC25A), signal transduction (e.g., MEK1) as well as genes associated with heat stress (e.g., Hsp70B', Hsp40). Gene expressions of CC3 and MEK1 were recognized to be induced by heat-shock in pharyngeal cancer cells (KB, T3M-1) and lingual cancer cells (SCC-TF).


CONCLUSIONS
The observed alteration in gene expression may play a role in various biochemical pathways of cancer cells exposed to heat-shock.
Narita
Norihiko
N
Department of Otorhinolaryngology, Fukui Medical University, Matsuoka, Fukui 910-1193, Japan. norihiko@fmsrsa.fukui-med.ac.jp
Noda
Ichiro
I
Ohtsubo
Toshio
T
Fujieda
Shigeharu
S
Tokuriki
Masaharu
M
Saito
Takehisa
T
Saito
Hitoshi
H
D000123:Acetyltransferases; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000628:therapy; D005334:Fever; D020869:Gene Expression Profiling / Q000379:methods*; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000628:therapy; D006801:Humans; D048369:MAP Kinase Kinase 1; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D014157:Transcription Factors; D000123:Acetyltransferases; C111246:HTATIP2 protein, human; D017346:Protein Serine-Threonine Kinases; D048369:MAP Kinase Kinase 1; C482125:MAP2K1 protein, human; D020929:Mitogen-Activated Protein Kinase Kinases