Type 1, P and S fimbriae, and afimbrial adhesin I are not essential for uropathogenic Escherichia coli to adhere to and invade bladder epithelial cells.
Fimbrial (type 1, P, and S) and afimbrial adhesins, the unique virulence traits of uropathogenic Escherichia coli (UPEC), are well recognized for their role in the initial step of uropathogenesis. In this study, we investigated whether these adhesins are dispensable for UPEC in adherence and invasion of uroepithelial cells by using E. coli isolates (n=40) from cystitis patients and T-24 cells, the bladder carcinoma cell line. We found all isolates adherent to T-24 cells within 15 min of infection. In invasion assay, all isolates could invade T-24 cells to a variable degree; 22.5% of them were found highly invasive. About 33% of isolates that do not have any recognized adhesins were as invasive as other isolates. The amplitude of invasiveness was also independent of the adhesins. In conclusion, this study demonstrates that type 1 fimbriae, P fimbriae, S fimbriae, and afimbrial adhesin I are not required for UPEC to adhere to and invade uroepithelial cells.
Miyazaki
Jun
J
Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba-shi, Ibaraki 305-8575, Japan. md995454@md.tsukuba.ac.jp
Ba-Thein
William
W
Kumao
Toshio
T
Obata Yasuoka
Mana
M
Akaza
Hideyuki
H
Hayshi
Hideo
H
D018830:Adhesins, Escherichia coli / Q000502:physiology*; D001422:Bacterial Adhesion / Q000502:physiology*; D001426:Bacterial Proteins / Q000502:physiology; D001427:Bacterial Toxins; D002295:Carcinoma, Transitional Cell / Q000473:pathology; D003556:Cystitis / Q000382:microbiology; D003603:Cytotoxins / Q000502:physiology; D004847:Epithelial Cells / Q000382:microbiology; D004926:Escherichia coli / Q000737:chemistry / Q000472:pathogenicity* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000502:physiology*; D005260:Female; D010861:Fimbriae, Bacterial / Q000502:physiology*; D006460:Hemolysin Proteins / Q000502:physiology; D006801:Humans; D008565:Membrane Proteins; D006899:Mixed Function Oxygenases / Q000502:physiology; D006180:Proton-Translocating ATPases; D014407:Tumor Cells, Cultured / Q000382:microbiology; D001743:Urinary Bladder / Q000382:microbiology*; D001749:Urinary Bladder Neoplasms / Q000473:pathology; D019459:Urothelium / Q000382:microbiology; D014774:Virulence
D018830:Adhesins, Escherichia coli; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003603:Cytotoxins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D008565:Membrane Proteins; C101637:ymcE protein, E coli; C060296:cytotoxic necrotizing factor type 1; D006899:Mixed Function Oxygenases; C047196:lysine N-epsilon hydroxylase; D006180:Proton-Translocating ATPases; C101709:AtpH protein, E coli