Introducing antisense oligonucleotides into Pneumocystis carinii.
To improve the knowledge on Pneumocystis carinii growth, a homologous P. carinii transformation system would provide a tool to promote replication of this fungus. Antisense oligonucleotides have been successfully introduced by electroporation or direct uptake in order to downregulate the prohibitin negative function on cell cycle.
de Monbrison
Frédérique
F
Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Hospices Civils de Lyon, E.A. 3087, Université Claude Bernard Lyon I, 69373, cedex 08, Lyons, France. fdmonb@rockefeller.univ-lyon1.fr
Picot
Stéphane
S
D000818:Animals; D002453:Cell Cycle; D018274:Electroporation / Q000379:methods*; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism*; D011010:Pneumocystis / Q000254:growth & development* / Q000378:metabolism; D011020:Pneumonia, Pneumocystis / Q000382:microbiology; D000091402:Prohibitins; D011506:Proteins / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins; D014170:Transformation, Genetic
D016376:Oligonucleotides, Antisense; D000091402:Prohibitins; D011506:Proteins; D012097:Repressor Proteins