Regulation of complement-mediated swine endothelial cell lysis by herpes simplex virus glycoprotein, gC1.
Herpes simplex virus type 1 (HSV-1) encodes several immuno-regulatory proteins that allow it to escape from the human immune system. The regulatory function of a HSV-1 glycoprotein gC (HSV-gC1) molecule on complement-mediated swine endothelial cell (SEC) lysis was investigated. The HSV-gC1 gene was obtained by the PCR method from the HSV-1 genome. The complement-regulatory function of this molecule was analyzed by cytotoxicity assay, using Chinese hamster ovarian tumor (CHO) cell and SEC transfectants and six human serum samples. FACS and Western blot analysis revealed the expression of the HSV-gC1 molecule on the transfectants. The CHO cell transfectants showed significant resistance to cell lysis by the sera that did not contain the anti-HSV-gC1 antibody. The SEC transfectants, however, showed a marked resistance to cell lysis in all cases. The introduction of a viral immune regulator such as HSV-gC1 into the swine cell provides a new approach for successful xenotransplantation.
Matsunami
Katsuyoshi
K
Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Miyagawa
Shuji
S
Fukuta
Daisuke
D
Shirakura
Ryota
R
D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D003179:Complement C3b / Q000276:immunology*; D006224:Cricetinae; D003412:Cricetulus; D003601:Cytotoxicity Tests, Immunologic; D015536:Down-Regulation; D004727:Endothelium / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D013552:Swine; D014162:Transfection; D014183:Transplantation, Heterologous / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; D003179:Complement C3b; D007770:L-Lactate Dehydrogenase