Effects of post-mortem delay and storage duration on the expression of GFAP in normal human adult retinae.
Glial fibrillary acidic protein (GFAP) is an established marker of retinal glia and has been shown to be modulated by several cytokines and retinal pathology. The influence of a number of factors, including post-mortem delay, storage duration and retinal pathology, on the distribution and morphology of macroglia and GFAP antigenicity was examined in human retina. The effects of these parameters on GFAP expression were estimated using immunohistochemistry, confocal microscopy and image analysis. Changes in expression of antigenicity were analysed in human retinal cryosections at three levels: constitutive,aberrant and total. The results indicated that short-term and long-term storage duration had no significant effect on GFAP immunoreactivity at all three levels of expression (P > 0.2).However, a significant increase in GFAP immunoreactivity and distribution at all three levels of expression was associated with prolonged post-mortem delay (> 30 h) (P < 0.05). This study highlights the importance of rigorous matching of post-mortem delay between control specimens in histological studies of human retinae. The study further demonstrates the utility of Eye Bank retinae fixed and stored in 2% paraformaldehyde, provided that appropriate controls are applied.
Wu
Kathy H C
KH
Department of Clinical Ophthalmology and Save Sight Institute, University of Sydney, Sydney, New South Wales, Australia. khcwu@eye.usyd.edu.au
Penfold
Philip L
PL
Billson
Francis A
FA
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D009457:Neuroglia / Q000378:metabolism; D009926:Organ Preservation; D012160:Retina / Q000378:metabolism*; D013997:Time Factors; D016707:Tissue Fixation; D020858:Tissue and Organ Harvesting / Q000379:methods*
D005904:Glial Fibrillary Acidic Protein