Comparison of chromogenic and fluorogenic membrane filtration methods for detection of four Candida species.
CHROMagar Candida medium was inoculated with a variety of clinical samples (n = 282) by using membrane filtration instead of swabbing or streaking. This modified CHROMagar procedure increased the ability to detect yeast by 16.7%. Compared to an enzymatic two-step membrane filtration method with fluorogenic substrates, it yielded similar recovery but was slower.
Bauters
T G
TG
Laboratory for Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University, Harlebekestraat 72, 9000 Ghent, Belgium.
Nelis
H J
HJ
D002175:Candida / Q000145:classification / Q000302:isolation & purification*; D002177:Candidiasis / Q000097:blood / Q000175:diagnosis; D003470:Culture Media; D004947:Esophagus / Q000382:microbiology; D005260:Female; D006801:Humans; D008567:Membranes, Artificial; D012680:Sensitivity and Specificity; D013997:Time Factors; D014132:Trachea / Q000382:microbiology; D014462:Ultrafiltration / Q000379:methods; D014621:Vagina / Q000382:microbiology
D003470:Culture Media; D008567:Membranes, Artificial