Inexpensive isolation of beta-D-glucopyranosidase from alpha-L-arabinofuranosidase, alpha-L-rhamnopyranosidase, and o-acetylesterase.
Beta-D-Glucopyranosidase (betaG, EC 3.2.1.21) has been isolated from some collateral activities, alpha-L-arabinofuranosidase (Ara, EC 3.2.1.55), alpha-L-rhamnopyranosidase (Rha, EC 3.2.1.40), and o-acetylesterase (Est, EC 3.1.1.53), using a commercial enzyme preparation and a simple method economically sustainable for the food industry. The procedure comprises precipitation of extraneous substances by adding ethanol and CaCl2, ultrafiltration, and adsorption, first on bentonite and then on chitosan. The results obtained were the complete isolation of betaG from the above-mentioned activities, a drastic reduction in extraneous compounds, such as brown substances and polysaccharides, and a slight increase in purification.
Barbagallo
Riccardo N
RN
University of Catania, Department of Horticulture, Floriculture, Arboriculture and Agroindustrial Technology, Italy. guiding@tiscalinet.it
Spagna
Giovanni
G
Abbate
Cristina
C
Azzaro
Giuseppe
G
Palmeri
Rosa
R
D000115:Acetylesterase / Q000302:isolation & purification / Q000378:metabolism; D000327:Adsorption; D011232:Chemical Precipitation; D002623:Chemistry Techniques, Analytical / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification; D013379:Substrate Specificity; D001617:beta-Glucosidase / Q000302:isolation & purification* / Q000378:metabolism
D011134:Polysaccharides; D000431:Ethanol; D000115:Acetylesterase; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; C022525:alpha-L-rhamnosidase; C019713:alpha-N-arabinofuranosidase