DNA vaccine against Friend erythroleukaemia virus.
Plasmids carrying DNA copies of the gag and env genes of FV, which causes erythroleukaemia in susceptible mouse strains, were prepared. Expression of the cloned genes was confirmed by indirect immunofluorescence in cells transfected in vitro. Immunization experiments were performed in DBA/2 mice. Animals were injected with three doses of plasmid either intramuscularly (100 microg DNA per dose) or intradermally (1 microg DNA per dose); in the latter case, a gene gun was used. The FV type A or P was used as a challenge. The immunization with gag- and env-derived vaccines resulted in protective immunity in a high proportion of mice.
Síma
P
P
Institute of Haematology and Blood Transfusion, Department of Experimental Virology, Prague, Czech Republic.
Smahel
M
M
Jelínek
F
F
Vonka
V
V
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D019470:Biolistics; D002460:Cell Line; D005622:Friend murine leukemia virus / Q000235:genetics / Q000276:immunology*; D015686:Gene Products, env / Q000235:genetics / Q000378:metabolism; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009929:Organ Size; D012192:Retroviridae Infections / Q000276:immunology / Q000517:prevention & control*; D013154:Spleen / Q000821:virology; D013155:Spleen Focus-Forming Viruses / Q000276:immunology; D014162:Transfection; D014611:Vaccination; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D015686:Gene Products, env; D015683:Gene Products, gag; D019444:Vaccines, DNA; D014765:Viral Vaccines