Qualitative and quantitative reversed-phase liquid chromatography of a new bisisoxazolylnaphthoquinone.
The main objective of this study was to develop and test the applicability of a sensitive, accurate, and precise liquid chromatographic (LC) method for evaluating the stability characteristics of a new bisisoxazolylnaphthoquinone, 2-(3,5-dimethyl-4-isoxazolylamino)-N-(3,5-dimethyl-4-isoxazolyl)-1,4-naphthoquinone-4-imine compound 1. The method was shown to be selective and stability-indicating. Isocratic elution with a mobile phase of methanol-water (75 + 25, v/v) on a reversed-phase column with UV detection at ambient temperature completely resolved compound 1 from its degradation products. The LC system was calibrated by plotting peak responses versus known concentrations of a reference standard by using an internal standardization procedure. Complete elution occurred after 12 min with a peak symmetry factor of 0.95 for the drug peak. The kinetic degradation of compound 1 was studied over a pH range of 0.88-14.00 to determine the kinetic parameters involved in its decomposition path in aqueous solution.
Ferreyra
Carola F
CF
Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Farmacia, Argentina.
Ortiz
Cristina S
CS
de Bertorello
María M
MM
D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009285:Naphthoquinones / Q000032:analysis* / Q000737:chemistry; D009994:Osmolar Concentration; D012680:Sensitivity and Specificity
D009285:Naphthoquinones