title: Adaptive correction of depth-induced aberrations in multiphoton scanning microscopy using a deformable mirror.
abstract: We demonstrate adaptive aberration correction for depth-induced spherical aberration in a multiphoton scanning microscope with a micromachined deformable mirror. Correction was made using a genetic learning algorithm with two-photon fluorescence intensity feedback to determine the desired shape for an adaptive mirror. For a 40x/0.6 NA long working distance objective, the axial scanning range was increased from 150 mm to 600 mm.
authors: Center for Ultrafast Optical Science, 1006 IST Bldg, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI 48109, USA.
authors: L
authors: L
authors: Sherman
authors: J Y
authors: JY
authors: Ye
authors: O
authors: O
authors: Albert
authors: T B
authors: TB
authors: Norris
mesh_terms: D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D055095:Optics and Photonics; D017785:Photons; D011791:Quartz / Q000378:metabolism
chemical_list: D011791:Quartz