Tetrazolium microplate assay as a rapid and inexpensive colorimetric method for determination of antibiotic susceptibility of Mycobacterium tuberculosis.
The emergence of multidrug-resistant tuberculosis underscores the need for low-cost, rapid methods to determine the susceptibility of Mycobacterium tuberculosis to antibiotics. A new, rapid, easily read, and inexpensive colorimetric method with a tetrazolium indicator performs this determination as quickly and accurately as the more expensive Alamar Blue technique.
Caviedes
Luz
L
Infectious Diseases Laboratory, Pathology Department, Universidad Peruana Cayetano Heredia, Lima, Peru.
Delgado
Jose
J
Gilman
Robert H
RH
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003124:Colorimetry / Q000191:economics / Q000379:methods; D003365:Costs and Cost Analysis; D008826:Microbial Sensitivity Tests / Q000191:economics / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D010568:Peru; D015203:Reproducibility of Results
D000900:Anti-Bacterial Agents