Rhamnose-binding lectins from steelhead trout (Oncorhynchus mykiss) eggs recognize bacterial lipopolysaccharides and lipoteichoic acid.
The interaction between bacteria and three L-rham-nose-binding lectins, named STL1, STL2, and STL3, from steelhead trout (Oncorhynchus mykiss) eggs was investigated. Although STLs bound to most Gram-negative and Gram-positive bacteria, they agglutinated only Escherichia coli K-12 and Bacillus subtilis among the bacteria tested. The binding was inhibited by L-rhamnose. STLs bound to distinct serotypes of lipopolysaccharides (LPSs), and showed much higher binding activities to smooth-type LPSs of Escherichia coli K-12 and Shigella flexneri 1A than to their corresponding rough-type LPSs. STLs also bound to lipoteichoic acid (LTA) of Bacillus subtilis. These results indicate that STLs bound to bacteria by recognizing LPSs or LTA on the cell surfaces.
Tateno
Hiroaki
H
Department of Biological Resource Sciences, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Ogawa
Tomohisa
T
Muramoto
Koji
K
Kamiya
Hisao
H
Saneyoshi
Mineo
M
D000371:Agglutination / Q000187:drug effects; D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000276:immunology / Q000378:metabolism*; D010063:Ovum / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D012210:Rhamnose / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D013682:Teichoic Acids / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
D002241:Carbohydrates; D037102:Lectins; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid; D012210:Rhamnose