[Regulatory effect of Bcl-2 on the cortical neurons of primary cultured mice with HSV-1 infection].
OBJECTIVE
To study the effect of Bcl-2 on the cortical neurons of primary cultured mice with HSV-1 infection.


METHODS
Analysis of the expression of Bcl-2 on the cortical neurons of primary cultured fetal mice in vitro infected with HSV-1 for 11 hours or exposed to sorbitol for 5 hours was made by flow cytometry and Western blotting.


RESULTS
The Bcl-2 of the cultured neurons undergoing HSV-1 infection expressed upregulating compared with the mock untreated neurons. The Bcl-2 protein of HSV-1 infected and exposed to sorbitol neurons expressed upregulating compared with the control group. The Bcl-2 protein of the primary cultured 3 day cells expressed higher than that of the primary cultured 7 day cells. The higher neurovirulence, the higher expression of Bcl-2.


CONCLUSIONS
Bcl-2 might protect the primary cultured cortical neurons of fetal mice from apoptosis where infected with HSV-1, and thereby the lifespan of host cells may be prolonged.
Wang
J
J
Department of Neurology, Beijing Friendship Hospital, Beijing 100050, China.
Wang
D
D
Feng
Z
Z
Wang
R J
RJ
Lu
Y
Y
Shen
J
J
Xu
Y
Y
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000821:virology; D005260:Female; D005333:Fetus; D006561:Herpes Simplex / Q000378:metabolism / Q000473:pathology; D018259:Herpesvirus 1, Human / Q000235:genetics; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000821:virology; D011247:Pregnancy; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics
D019253:Proto-Oncogene Proteins c-bcl-2