[Analysis of the nucleotide sequence for C and NS5 regions and the genotype of HCV isolate in Shandong Province].
OBJECTIVE
To study the genotype of HCV epidemic strains in Shandong Province.


METHODS
HCV fragments of C(432 bp) and NS5(319 bp) regions were amplified by RT nested PCR. The fragments were cloned to T vector and sequenced by dideoxynucleotide chain termination reaction.


RESULTS
Three of all 4 strains in C region were 1b genotype, and 1 was 2a. All of 10 strains of NS5 region were 1b genotype. No other genotype was found in this region. The homology of nucleotide sequence was over 90% compared with that of more than 50 strains in Gen Bank.


CONCLUSIONS
The major genotype of HCV epidemic strains in Shandong Province is 1b. The 2a genotype is also found in this area.
Zhang
W
W
Key Laboratory of Molecular Virology, Medical College of Qingdao University, Qiangdao 266021, China.
Yu
H
H
Wang
X
X
Wang
Q
Q
Lu
R
R
Wang
H Q
HQ
Shao
J J
JJ
D005838:Genotype; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014758:Viral Core Proteins / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase
D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; C069130:nucleocapsid protein, Hepatitis C virus; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase