Truncated apoE forms tangle-like structures in a neuronal cell line.
Apolipoprotein E is the predominant brain lipoprotein and polymorphic variation in the APOE gene the major genetic susceptibly factor for late onset Alzheimer's disease (AD). Recently it was reported that carboxyl-truncated ApoE fragments induce tangle-like structures in neurons. We confirm the finding: in mouse neuroblastoma cells truncated apoE fragments lacking the carboxyterminus induce structures that have the appearance of neurofibrillary tangles. However these tangles are not induced in non-neuronal cells even in the presence of co-expressed neurofilaments or tau. Further understanding of the basis of this cell specificity might add to understanding of the cell specificity of tangles in AD.
Ljungberg
M Cecilia
MC
Department of Neuroscience, Institute of Psychiatry, King's College London, De Crespigny Park, London SE5 8AF, UK.
Dayanandan
Rejith
R
Asuni
Ayodeji
A
Rupniak
Tom H
TH
Anderton
Brian H
BH
Lovestone
Simon
S
D000544:Alzheimer Disease / Q000378:metabolism* / Q000503:physiopathology; D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency* / Q000235:genetics*; D001921:Brain / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D016466:CHO Cells; D019556:COS Cells; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D005333:Fetus; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000378:metabolism; D051379:Mice; D016874:Neurofibrillary Tangles / Q000378:metabolism* / Q000473:pathology; D016900:Neurofilament Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D010446:Peptide Fragments / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000502:physiology; D022161:Transport Vesicles / Q000378:metabolism; D014407:Tumor Cells, Cultured; D016875:tau Proteins / Q000378:metabolism
D001057:Apolipoproteins E; D018076:DNA, Complementary; D016900:Neurofilament Proteins; D010446:Peptide Fragments; D021382:Protein Sorting Signals; D016875:tau Proteins