title: Inhibition of haem oxygenase activity increases leukocyte accumulation in the liver following limb ischaemia-reperfusion in mice.
abstract: The role of haem oxygenase (HO) in the hepatic accumulation of leukocytes in mice during the initiation of remote organ injury following normotensive limb ischaemia-reperfusion (I-R) was investigated. Remote organ injury was initiated by 1 h bilateral hindlimb ischaemia followed by either 1 or 1.5 h reperfusion (I-R) in male C57BL/6 mice. Mice were randomly assigned to either sham (no I-R, n = 4), I-R (n = 4 for both time points), I-R plus chromium mesoporphyrin (CrMP, n = 4) to inhibit HO or I-R plus haemin (n = 4) to increase HO. Leukocyte accumulation and leukocyte-endothelial interaction were directly measured using fluorescence intravital microscopy. Leukocytes were labelled via an injection of rhodamine 6G. In sinusoids the total number and the number of stationary leukocytes were assessed. In postsinusoidal venules the number of adherent and rolling leukocytes and the velocities of both red blood cells and leukocytes were measured. The total number of leukocytes increased in sinusoids of I-R mice reaching a plateau within 1 h compared with sham animals, while the number of stationary leukocytes progressively increased over the entire study period. Stationary leukocytes in sinusoids increased after 1 and 1.5 h of I-R following CrMP, while they were significantly reduced following haemin treatment compared to animals treated with I-R only. In postsinusoidal venules a progressive increase in adherent leukocytes also occurred. As observed in sinusoids, CrMP significantly increased, while haemin significantly reduced leukocyte adhesion. The number of rolling leukocytes increased after CrMP in both I-R groups (1 and 1.5 h). The velocities of rolling leukocytes declined following 1.5 h of I-R compared with sham. Haemin treatment of 1.5 h I-R animals restored the velocities back to sham levels. The calculated wall shear rates in postsinusoidal venules were significantly lower in all I-R groups in comparison to sham animals. Combination of 1.5 h I-R with CrMP resulted in the lowest shear rates of all I-R groups. The number of stationary leukocytes within sinusoids and adherent leukocytes in postsinusoidal venules were correlated to the corresponding alanine aminotransferase (ALT) levels. In conclusion, endogenous HO reduces leukocyte-endothelial interactions within the liver. Thus, endogenous HO activity provides an important mechanism controlling the hepatic inflammatory response during the initiation of remote organ injury following normotensive limb ischaemia-reperfusion.
authors: Klinik für Anaesthesiologie, Julius-Maximilians-Universität Würzburg, Germany.
authors: Christian
authors: C
authors: Wunder
authors: Robert W
authors: RW
authors: Brock
authors: Sarah D
authors: SD
authors: McCarter
authors: Aurelia
authors: A
authors: Bihari
authors: Kenneth
authors: K
authors: Harris
authors: Otto
authors: O
authors: Eichelbrönner
authors: Richard F
authors: RF
authors: Potter
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers; D002448:Cell Adhesion; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005121:Extremities / Q000098:blood supply*; D006419:Heme Oxygenase (Decyclizing) / Q000037:antagonists & inhibitors*; D007511:Ischemia / Q000503:physiopathology; D007962:Leukocytes / Q000502:physiology*; D008099:Liver / Q000473:pathology / Q000502:physiology*; D008297:Male; D008652:Mesoporphyrins / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D015424:Reperfusion; D015427:Reperfusion Injury / Q000503:physiopathology*; D014699:Venules / Q000502:physiology
chemical_list: D015415:Biomarkers; D004791:Enzyme Inhibitors; D008652:Mesoporphyrins; C427471:chromium mesoporphyrin; D006419:Heme Oxygenase (Decyclizing); D000410:Alanine Transaminase