title: Characterization of plasmid MIP233 (IncHI3) of the H complex.
abstract: Plasmid MIP233, the unique member of the IncHI3 subgroup of the H incompatibility complex, is a large self-transferable plasmid encoding for sucrose utilization, the PacB character and resistance to tellurite. The current study examined whether pMIP233 conferred other putatively advantageous plasmid-encoded functions. Heavy metal studies revealed pMIP233 phenotypes of resistance to cadmium, cobalt, lead, zinc and nickel. Sequencing of random clones from a pMIP233 library revealed extensive homology with IS elements and genes involved in the metabolic processes demonstrated. Interestingly, pMIP233 did not appear to code for resistance to a wide range of antibiotics. pMIP233 is considered to be a metabolic plasmid.
authors: Instituto de Biología Experimental, Facultad de Ciencias, Universidad Central de Venezuela, Caracas. galonso@reacciun.ve
authors: Guillermina
authors: G
authors: Alonso
authors: Glenda
authors: G
authors: Vílchez
authors: Igor
authors: I
authors: Bruzual
authors: Vidal
authors: V
authors: Rodríguez-Lemoine
mesh_terms: D001419:Bacteria / Q000187:drug effects; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics*; D008670:Metals / Q000494:pharmacology*; D016366:Open Reading Frames; D010957:Plasmids; D013395:Sucrose / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D008670:Metals; D013395:Sucrose