Isolation and characterization of a novel postsynaptic/cytotoxic neurotoxin from Daboia russelli russelli venom.
A postsynaptic neurotoxin was purified from Daboia russelli russelli venom using gel filtration, ion-exchange chromatography and reverse-phase high-performance liquid chromatography. The N-terminal sequence, molecular mass and pharmacological activities of the neurotoxin/cytotoxin indicate that it is a short-chain neurotoxin like that found in Elapid venom. This is the first report on the presence of such a postsynaptic neurotoxin from D. r. russelli venom.
Shelke
R R J
RR
Biochemistry of Aging Laboratory, College of Health and Human Performance, Gainesville, FL 3211-8205, USA. shelker@ufl.edu
Sathish
S
S
Gowda
T V
TV
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D007928:Lethal Dose 50; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009046:Motor Neurons / Q000187:drug effects; D009132:Muscles / Q000187:drug effects; D009498:Neurotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity*; D011898:Ranidae; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013569:Synapses / Q000187:drug effects*; D018675:Toxicity Tests; D014757:Viper Venoms / Q000737:chemistry*; D017819:Viperidae
D009498:Neurotoxins; D014757:Viper Venoms