[HIV genomic mutation on antiretroviral therapy].
BACKGROUND
To study the characteristics of HIV genomic mutation on antiretroviral therapy.


METHODS
HIV DNA and HIV RNA were detected by using Taq-PCR, RT-PCR, LD-PCR, hybridization, and oligonucleotide 32P- probes against gag, tat, nef, LTRU3, etc. RESULYS: The HIV RNA load in serum declined significantly, CD4 lymphocyte count increased, copies of gag region of HIV DNA decreased, and full-length HIV DNA disappeared. Bands showing gag segments hybridized with 32P-probe appeared negative or less clear in some cases after antiretroviral therapy. However, the regions hybridized with probes Xho-nef and LTR U5 were apparently enhanced and some drawbands were seen.


CONCLUSIONS
HIV gene may experiece obvious mutation on antiretroviral therapy.
Xu
X
X
Department of Infectious Diseases, the First Clinic Hospital, Beijing Medical University, 100034 Beijing, China.
Xing
H
H
Chermannn
J C
JC
D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D005260:Female; D005814:Genes, Viral / Q000235:genetics*; D006678:HIV / Q000235:genetics*; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation