Analysis of fumonisin B(1) in Fusarium proliferatum-infected asparagus spears and garlic bulbs from Germany by liquid chromatography-electrospray ionization mass spectrometry.
Fusarium proliferatum is one of a group of fungal species that produce fumonisins and is considered to be a pathogen of many economically important plants. The occurrence of fumonisin B(1) (FB(1)) in F. proliferatum-infected asparagus spears from Germany was investigated using a liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) method with isotopically labeled fumonisin FB(1)-d(6) as internal standard. FB(1) was detected in 9 of the 10 samples in amounts ranging from 36.4 to 4513.7 ng/g (based on dry weight). Furthermore, the capability of producing FB(1) by the fungus in garlic bulbs was investigated. Therefore, garlic was cultured in F. proliferatum-contaminated soil, and the bulbs were screened for infection with F. proliferatum and for the occurrence of fumonisins by LC-MS. F. proliferatum was detectable in the garlic tissue, and all samples contained FB(1) (26.0-94.6 ng/g). This is the first report of the natural occurrence of FB(1) in German asparagus spears, and these findings suggest a potential for natural contamination of garlic bulbs with fumonisins.
Seefelder
Walburga
W
Lehrstuhl für Lebensmittelchemie, Universität Würzburg, Am Hubland, 97074 Würzburg, Germany.
Gossmann
Monika
M
Humpf
Hans-Ulrich
HU
D027761:Asparagus Plant / Q000737:chemistry* / Q000382:microbiology; D002264:Carboxylic Acids / Q000032:analysis*; D002853:Chromatography, Liquid; D005506:Food Contamination; D037341:Fumonisins; D005670:Fusarium / Q000378:metabolism*; D005737:Garlic / Q000737:chemistry* / Q000382:microbiology; D005858:Germany; D021241:Spectrometry, Mass, Electrospray Ionization
D002264:Carboxylic Acids; D037341:Fumonisins; C056933:fumonisin B1