Spectral surface plasmon resonance biosensor for detection of staphylococcal enterotoxin B in milk.
This work evaluates a newly developed wavelength modulation-based SPR biosensor for the detection of staphylococcal enterotoxin B (SEB) in milk. Two modes of operation of the SPR biosensor are described: direct detection of SEB and sandwich assay. In the sandwich assay detection mode, secondary antibodies are bound to the already captured toxin to amplify sensor response. Samples including SEB in buffer and SEB in milk were analyzed in this work. The SPR biosensor has been shown to be capable of directly detecting concentrations of SEB in buffer as low as 5 ng/ml. In sandwich detection mode, the lowest detection limit was determined to be 0.5 ng/ml for both buffer and milk samples. The reported wavelength modulation-based SPR sensor provides a generic platform which can be tailored for detection of various foodborne pathogens and agents for food analysis and testing.
Homola
Jirí
J
Department of Electrical Engineering, University of Washington, Seattle 98195, USA. homola@ee.washington.edu
Dostálek
Jakub
J
Chen
Shengfu
S
Rasooly
Avraham
A
Jiang
Shaoyi
S
Yee
Sinclair S
SS
D000818:Animals; D000907:Antibodies, Bacterial; D015374:Biosensing Techniques; D004768:Enterotoxins / Q000032:analysis / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D008892:Milk / Q000382:microbiology*; D013202:Staphylococcal Food Poisoning / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
D000907:Antibodies, Bacterial; D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal