title: Immune responses in mice to intranasal and intracutaneous administration of a DNA vaccine encoding Helicobacter pylori-catalase.
abstract: We previously reported that the intracutaneous injection of DNA vaccines encoding Helicobacter pylori heat shock proteins elicited specific immune responses, and led to reduced infection in mice. In this study, we constructed DNA vaccine encoding H. pylori-catalase (pcDNA3.1-kat) and investigated the immune responses to intranasal and intracutaneous administration of pcDNA3.1-kat. C57/BL6 mice were immunized intracutaneously with 10 microg of pcDNA3.1-kat or intranasally with 50 microg of pcDNA3.1-kat. Catalase-specific IgG antibody was detected in the sera of intranasal and intracutaneous immunized mice. Both intranasal and intracutaneous immunized mice were significantly protected from colonization by H. pylori and had significantly reduced degrees of gastritis. These results demonstrate that DNA vaccine encoding H. pylori-catalase can induce an immune response against H. pylori, and that intranasal immunization works as well as intracutaneous immunization.
authors: The Second Department of Internal Medicine, Aichi Medical University, 21 Karimata, Yazako, Nagakute-Cho, Aichi, Japan.
authors: Masayuki
authors: M
authors: Miyashita
authors: Takashi
authors: T
authors: Joh
authors: Katsushi
authors: K
authors: Watanabe
authors: Isami
authors: I
authors: Todoroki
authors: Kyoji
authors: K
authors: Seno
authors: Hirotaka
authors: H
authors: Ohara
authors: Tomoyuki
authors: T
authors: Nomura
authors: Mitsuki
authors: M
authors: Miyata
authors: Kunio
authors: K
authors: Kasugai
authors: Kunio
authors: K
authors: Tochikubo
authors: Makoto
authors: M
authors: Itoh
authors: Masakazu
authors: M
authors: Nitta
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002374:Catalase / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D002461:Cell Line, Transformed; D005260:Female; D015870:Gene Expression; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D013270:Stomach / Q000276:immunology / Q000473:pathology; D019444:Vaccines, DNA / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D014765:Viral Vaccines; D002374:Catalase