title: Inhibition of 4-nitroquinoline-1-oxide genotoxicity by Bacillus strains.
abstract: The effect of 16 Bacillus strains from pharmaceutical probiotic preparations (Bacillus spp.) and collection (B. subtilis, B. firmus, B. megaterium, B. pumilus) on genotoxicity induced by the known mutagen 4-nitroquinoline-1-oxide (4-NQO) was studied using the short-term bacterial assay SOS-chromotest. with Escherichia coli PQ37 as the tester organism. It was found that the activity of 0.1 mM 4-NQO was reduced (P < 0.01) after coincubation with Bacillus suspensions (10(8) CFU/ml for 150 min at 37 degrees C). All isolates showed potential for deactivating 4-NQO, and genotoxicity inhibition ranged from 92.9 to 100%. There were no appreciable differences in behaviour observed among probiotic and collection strains or in relation to species. The observed antigenotoxicity was associated with a clear-cut modification of 4-NQO molecular characteristics.
authors: Dipartimento di Biologia Cellulare e Molecolare, Università di Perugia, Italy.
authors: Giovanna
authors: G
authors: Caldini
authors: Francesca
authors: F
authors: Trotta
authors: Giovanni
authors: G
authors: Cenci
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000737:chemistry / Q000633:toxicity*; D000469:Alkaline Phosphatase / Q000378:metabolism; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001407:Bacillus / Q000502:physiology*; D019936:Probiotics / Q000494:pharmacology*; D013014:SOS Response, Genetics / Q000187:drug effects; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D016587:Antimutagenic Agents; D015112:4-Nitroquinoline-1-oxide; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase