Helicobacter pylori and acetylsalicylic acid synergistically accelerate apoptosis via Fas antigen pathway in rabbit gastric epithelial cells.
The mechanism for gastric epithelial cellular apoptosis by both H. pylori and NSAIDs remains unknown. Normal gastric epithelial cells, collected from rabbit stomach, were cultured with viable H. pylori and/or an acetylsalicylic acid for 24 hr as an in vitro model of gastric epithelial cell injury. The Fas antigen expression rate in the gastric epithelial cells was measured using a FACScan. In group E, in which H. pylori inoculation of epithelial cells was followed by treatment with an NSAID, the rate of Fas antigen expression was significantly higher than the rate observed after treatment with either H. pylori or NSAID alone. Despite the fact that drug treatment alone did not significantly increase the Fas expression rate vs control cells. The results suggest that H. pylori and NSAID induce gastric cell injury as apoptosis via the Fas antigen pathway in a synergistic manner and that this effect is particularly strong when NSAID treatment follows H. pylori infection.
Watanabe
Kazuhiro
K
Third Department of Internal Medicine, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.
Hoshiya
Satoshi
S
Tokunaga
Kengo
K
Tanaka
Akifumi
A
Watanabe
Hidehiro
H
Nagamatsu
Shinya
S
Ishida
Hitoshi
H
Takahashi
Shinichi
S
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000942:Antigens, Bacterial; D017209:Apoptosis / Q000187:drug effects*; D001241:Aspirin / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D002460:Cell Line; D053938:DNA Fragmentation; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology* / Q000503:physiopathology; D016481:Helicobacter Infections / Q000503:physiopathology*; D016480:Helicobacter pylori / Q000235:genetics; D015151:Immunoblotting; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D011817:Rabbits; D019014:fas Receptor / Q000502:physiology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; D019014:fas Receptor; D007770:L-Lactate Dehydrogenase; D001241:Aspirin