Use of reverse transcriptase PCR in early diagnosis of Rift Valley fever.
Reverse transcriptase PCR (RT-PCR) for diagnosis of Rift Valley fever (RVF) was evaluated by using 293 human and animal sera sampled during an RVF outbreak in Mauritania in 1998. Results of the RT-PCR diagnostic method were compared with those of virus isolation (VI) and detection of immunoglobulin M (IgM) antibodies. Our results showed that RT-PCR is a specific, sensitive tool for RVF diagnosis in the early phase of the disease and that its results do not differ significantly from those obtained by VI. Moreover, the combined results of RT-PCR and IgM antibody detection were in 100% concordance with the results of VI.
Sall
A A
AA
Institut Pasteur de Dakar, Dakar, Senegal. sall@pasteur.fr
Macondo
E A
EA
Sène
O K
OK
Diagne
M
M
Sylla
R
R
Mondo
M
M
Girault
L
L
Marrama
L
L
Spiegel
A
A
Diallo
M
M
Bouloy
M
M
Mathiot
C
C
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002162:Camelus; D002417:Cattle; D002460:Cell Line; D004196:Disease Outbreaks; D005602:France / Q000453:epidemiology; D006041:Goats; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D012367:RNA, Viral / Q000097:blood*; D012194:RNA-Directed DNA Polymerase; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012295:Rift Valley Fever / Q000175:diagnosis* / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D012296:Rift Valley fever virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D012756:Sheep; D017361:Viral Nonstructural Proteins / Q000235:genetics
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D012194:RNA-Directed DNA Polymerase