Copper transfer from the Cu(I) chaperone, CopZ, to the repressor, Zn(II)CopY: metal coordination environments and protein interactions.
Extracellular copper regulates the DNA binding activity of the CopY repressor of Enterococcus hirae and thereby controls expression of the copper homeostatic genes encoded by the cop operon. CopY has a CxCxxxxCxC metal binding motif. CopZ, a copper chaperone belonging to a family of metallochaperones characterized by a MxCxxC metal binding motif, transfers copper to CopY. The copper binding stoichiometries of CopZ and CopY were determined by in vitro metal reconstitutions. The stoichiometries were found to be one copper(I) per CopZ and two copper(I) per CopY monomer. X-ray absorption studies suggested a mixture of two- and three-coordinate copper in Cu(I)CopZ, but a purely three-coordinate copper coordination with a Cu-Cu interaction for Cu(I)2CopY. The latter coordination is consistent with the formation of a compact binuclear Cu(I)-thiolate core in the CxCxxxxCxC binding motif of CopY. Displacement of zinc, by copper, from CopY was monitored with 2,4-pyridylazoresorcinol. Two copper(I) ions were required to release the single zinc(II) ion bound per CopY monomer. The specificity of copper transfer between CopZ and CopY was dependent on electrostatic interactions. Relative copper binding affinities of the proteins were investigated using the chelator, diethyldithiocarbamic acid (DDC). These data suggest that CopY has a higher affinity for copper than CopZ. However, this affinity difference is not the sole factor in the copper exchange; a charge-based interaction between the two proteins is required for the transfer reaction to proceed. Gain-of-function mutation of a CopZ homologue demonstrated the necessity of four lysine residues on the chaperone for the interaction with CopY. Taken together, these results suggest a mechanism for copper exchange between CopZ and CopY.
Cobine
Paul A
PA
National Research Center for Environmental Toxicology, University of Queensland, Coopers Plains, QLD 4108, Australia.
George
Graham N
GN
Jones
Christopher E
CE
Wickramasinghe
Wasantha A
WA
Solioz
Marc
M
Dameron
Charles T
CT
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D001667:Binding, Competitive; D003300:Copper / Q000378:metabolism*; D016983:Enterococcus / Q000378:metabolism*; D008958:Models, Molecular; D018832:Molecular Chaperones; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D011487:Protein Conformation; D012097:Repressor Proteins / Q000737:chemistry* / Q000378:metabolism*; D017186:Titrimetry; D015534:Trans-Activators / Q000737:chemistry* / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
D001426:Bacterial Proteins; C092378:CopY protein, Enterococcus hirae; C092379:CopZ protein, Enterococcus hirae; D018832:Molecular Chaperones; D012097:Repressor Proteins; D015534:Trans-Activators; D003300:Copper; D015032:Zinc