A novel piezoelectric biosensor for the detection of phytohormone beta-indole acetic acid.
A novel piezoelectric immunosensor has been developed for the determination of beta-indole acetic acid (IAA) in dilute solutions. The detection is based on competitive immunoreaction between a hapten (IAA) and an antigen (IAA-BSA, hapten-protein conjugation) bound to an anti-IAA antibody, immobilized on a quartz crystal microbalance (QCM). The frequency change (y) of the sensor caused by antigen is linearly related to the logarithm of the concentration of IAA (x) in the range of 0.5 ng/ml - 5 microg/ml with a regression equation of the form y = -23x + 151 (r = 0.9937).
Li
Jin
J
State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Department of Environmental Science and Engineering, Hunan University, ChangSha, P R China.
Wu
Zhao-Yang
ZY
Xiao
Lang-Tao
LT
Zeng
Guang-Ming
GM
Huang
Guo-He
GH
Shen
Guo-Li
GL
Yu
Ru-Qin
RQ
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002138:Calibration; D007118:Immunoassay / Q000379:methods; D018796:Immunoconjugates / Q000276:immunology; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000378:metabolism; D007210:Indoleacetic Acids / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D010937:Plant Growth Regulators / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D011791:Quartz / Q000378:metabolism; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000378:metabolism; D013997:Time Factors
D018796:Immunoconjugates; D007074:Immunoglobulin G; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D011791:Quartz; D012710:Serum Albumin, Bovine; C030737:indoleacetic acid