Expression, proteolysis and activation of caspases 6 and 7 during rat C6 glioma cell apoptosis.
Activation of cysteinyl aspartate-specific proteases (caspases) may underlie apoptotic cell death in brain. Terminal, executioner caspases 3, 6 and 7 likely contribute to such cell death in a stimulus- and cell type-specific manner. Here we investigate the processing and activation of caspases 3, 6 and 7 in rat C6 glioma cells induced to undergo apoptosis by staurosporine (STS) treatment as a model of apoptosis in glia. Proteolysis and activation of caspases 3 and 7 as determined by immunoblotting and substrate-specific cleavage assay (DEVDase) preceded caspase-6 proteolysis and increased VEIDase activity following STS treatment. Activation of caspase-6 was paralleled by cleavage of the nuclear envelope protein lamin-A. These results highlight temporal differences in the activation of the triad of executioner caspases 3, 6 and 7 during glial cell apoptosis.
Meller
Robert
R
R.S. Dow Neurobiology Laboratories, Legacy Clinical Research and Technology Center, 1225 NE 2nd Avenue, Portland, OR 97232, USA. rmeller@downeurobiology.org
Skradski
Shana L
SL
Simon
Roger P
RP
Henshall
David C
DC
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001921:Brain / Q000201:enzymology* / Q000503:physiopathology; D001927:Brain Diseases / Q000201:enzymology* / Q000503:physiopathology; D053148:Caspase 3; D053178:Caspase 6; D053179:Caspase 7; D020169:Caspases / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005910:Glioma; D020287:In Situ Nick-End Labeling; D034904:Lamin Type A; D034882:Lamins; D009457:Neuroglia / Q000201:enzymology* / Q000473:pathology; D009687:Nuclear Proteins / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D051381:Rats; D019311:Staurosporine / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D004791:Enzyme Inhibitors; D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins; D010447:Peptide Hydrolases; C505410:Casp3 protein, rat; C505518:Casp6 protein, rat; D053148:Caspase 3; D053178:Caspase 6; D053179:Caspase 7; D020169:Caspases; D019311:Staurosporine