title: A decrease in cell size accompanies a loss of docosahexaenoate in the rat hippocampus.
abstract: Rats raised on n-3 essential fatty acid deficient diets demonstrate spatial memory deficits. To investigate neuroanatomical correlates of these deficits, morphological analysis of the hippocampus were carried out. Adult, female rats were raised for three generations on n-3 deficient or n-3 supplemented diets. Two n-3 deficient diets contained adequate linoleic acid (LA), or high linoleic acid (high LA), and two supplemented diets contained LA supplemented with alpha-linolenic acid (+LNA), or linoleic supplementation with alpha-linolenic and docosahexaenoic acids (+LNA/DHA). The total fatty acid composition of the hippocampus revealed a profound loss (90%) in docosahexaenoic acid (DHA) in the hippocampi of LA and high LA animals compared to those on +LNA and +LNA/DHA diets with a reciprocal increase in docosapentaenoic acid (DPAn-6) in all phospholipid species. The volume, density, total number, and cell body size of neurons in CA1-3, granular and hilar layers of the hippocampus were measured at septal and temporal locations using unbiased stereology. No differences were detected in any of these measures except for in cell body size; CA1 pyramidal neurons in the LA group were significantly (p < 0.04) smaller than neurons in the +LNA/DHA group at the septal location.
authors: Section of Nutritional Neuroscience, Laboratory of Membrane Biochemistry and Biophysics, Division of Intramural Clinical and Biological Research, National Institutes on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA.
authors: Aneeq
authors: A
authors: Ahmad
authors: Mahadev
authors: M
authors: Murthy
authors: Rebecca S
authors: RS
authors: Greiner
authors: Toru
authors: T
authors: Moriguchi
authors: Norman
authors: N
authors: Salem
mesh_terms: D000818:Animals; D002452:Cell Count; D048429:Cell Size; D004041:Dietary Fats / Q000008:administration & dosage; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D005260:Female; D006624:Hippocampus / Q000737:chemistry* / Q000166:cytology*; D019787:Linoleic Acid / Q000008:administration & dosage; D009474:Neurons / Q000166:cytology; D051381:Rats; D020318:Rats, Long-Evans; D017962:alpha-Linolenic Acid / Q000008:administration & dosage
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D017962:alpha-Linolenic Acid; D004281:Docosahexaenoic Acids; D019787:Linoleic Acid