Development of an improved method for production of antiscorpion F(ab')2 fragment of IgG with high yield and potency.
The main objective of the present work was to obtain a stable and highly purified scorpion antitixon, rich in specific antibodies, free of immunologically irrelevant plasma proteins or gross proteins. Our results indicate that the most potent yielded and purified F(ab')2 antivenom preparation was obtained when the first discarded precipitate was washed with 14% ammonium sulphate saline; then after the second addition of ammonium sulphate, the mixture was stirring overnight followed by precipitation of most non-immunoglobulin proteins with the aid of caprylic acid to produce antivenom rich in specific antibodies with higher yield and potency compared to the method commonly used.
Seddik
Salwa S
SS
Egyptian Organization for Biological Products and Vaccines (VACSERA), Agouza, Giza, Egypt. salwaseddik@hotmail.com
Wanas
Soheir
S
Shehata
Awatef
A
Fawaz
Shehata
S
Helmy
Madiha H
MH
D000818:Animals; D000997:Antivenins / Q000276:immunology / Q000302:isolation & purification*; D005591:Chemical Fractionation; D011232:Chemical Precipitation; D004355:Drug Stability; D006736:Horses; D007106:Immune Sera / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000276:immunology / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests; D012604:Scorpion Venoms / Q000276:immunology* / Q000633:toxicity
D000997:Antivenins; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D012604:Scorpion Venoms