Identification of differentially expressed genes induced by transient ischemic stroke.
We have used a rat model of focal cerebral ischemia to investigate changes in gene expression that occur during stroke. To monitor these changes, we employed representational difference analysis-polymerase chain reaction (PCR). A total of 128 unique gene fragments were isolated, and we selected 13 of these for quantitative reverse transcriptase-PCR analysis. Of these 13 genes, we found seven that were differentially expressed. Four of these genes have not previously been implicated in stroke, and include neuronal activity regulated pentraxin (Narp), cysteine rich protein 61 (Cyr61), Bcl-2 binding protein BIS (Bcl-2-interacting death suppressor), and lectin-like ox-LDL receptor (LOX-1). We demonstrated differential expression of each gene by quantitative PCR analysis, and in the case of LOX-1, we further confirmed differential expression by in situ hybridization. LOX-1 expression is induced greater than ten fold at the core lesion site, and is essentially localized to the ipsilateral half of the brain. LOX-1 appears to be expressed in a non-neuronal cell type, and it does not appear to be expressed in vascular endothelial cells within the brain. This suggests that LOX-1 may serve a novel function in the brain.
Schwarz
David A
DA
Neurocrine Biosciences, Inc., 10555 Science Center Drive, San Diego, CA 92121-1102, USA. dschwarz@neurocrine.com
Barry
Guy
G
Mackay
Kenneth B
KB
Manu
Frank
F
Naeve
Gregory S
GS
Vana
Alicia M
AM
Verge
Gail
G
Conlon
Paul J
PJ
Foster
Alan C
AC
Maki
Richard A
RA
D000818:Animals; D002545:Brain Ischemia / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D002544:Cerebral Infarction / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D018076:DNA, Complementary / Q000032:analysis; D004730:Endothelium, Vascular / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D017072:Neostriatum / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D009457:Neuroglia / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011973:Receptors, LDL / Q000235:genetics; D051131:Receptors, Oxidized LDL; D015427:Reperfusion Injury / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D051127:Scavenger Receptors, Class E; D020521:Stroke / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D015854:Up-Regulation / Q000502:physiology
D018076:DNA, Complementary; C495136:OLR1 protein, rat; D012333:RNA, Messenger; D011973:Receptors, LDL; D051131:Receptors, Oxidized LDL; D051127:Scavenger Receptors, Class E