Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs.
A PCR identification of methicillin-resistant Staphylococcus aureus (MRSA), obviating the need for subculture on agar media, was investigated. The combination of MRSA detection by mecA femB PCR with prior enrichment in selective broth was tested for 439 swabs. PCR identified 36 MRSA-positive samples, in concordance with conventional methods.
Jonas
D
D
National Reference Center of Hygiene, Institute of Environmental Medicine and Hospital Epidemiology, University Hospital Freiburg, Hugstetter Strasse 55, 79106 Freiburg, Germany. djonas@IUK3.UKL.uni-Freiburg.de
Speck
M
M
Daschner
F D
FD
Grundmann
H
H
D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics; D017931:DNA Primers; D006602:Hexosyltransferases; D006801:Humans; D016106:Methicillin Resistance; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D017931:DNA Primers; C102928:FemB protein, Staphylococcus epidermidis; D046915:Penicillin-Binding Proteins; C083616:mecA protein, Bacillus subtilis; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase