title: Multiple rearrangements of mitochondrial DNA in unfertilized human oocytes.
abstract: OBJECTIVE
  To determine the rearrangement of mitochondrial DNA (mtDNA) in unfertilized human oocytes and compromised embryos to evaluate the fertilization capacity of oocytes.
  
  
  METHODS
  Prospective laboratory research.
  
  
  METHODS
  IVF laboratory in a university hospital.
  
  
  METHODS
  One hundred twenty-four unfertilized oocytes, 98 arrested embryos, and 45 tripronucleate (3PN) embryos from 65 female patients undergoing in vitro fertilization (IVF).
  
  
  METHODS
  Unfertilized oocytes and poor quality embryos were collected 48 hours after IVF.
  
  
  METHODS
  Comparison of the frequency of mtDNA deletions and fertilization rates of oocytes.
  
  
  RESULTS
  Multiple deletions of mtDNA were found in unfertilized oocytes and arrested embryos obtained from IVF patients. A 4977-bp deletion was the most frequent deletion in human oocytes and embryos. About 66.1% of the unfertilized oocytes, 34.8% of the arrested or fragmented embryos, and 21.1% of the 3PN embryos harbored the 4977-bp deletion of mtDNA. There was a significant increase in the proportion of deleted mtDNA in unfertilized oocytes.
  
  
  CONCLUSIONS
  Accumulation of mtDNA deletions may contribute to mitochondrial dysfunction and impaired ATP production. We conclude that the accumulation of rearranged mtDNA may interfere with fertilization of human oocytes and further embryonic development.
authors: Center for Reproductive Medicine and Science, Taipei Medical University, Taipei, Taiwan.
authors: Rong-Hong
authors: RH
authors: Hsieh
authors: Nu-Man
authors: NM
authors: Tsai
authors: Heng-Kien
authors: HK
authors: Au
authors: Shu-Ju
authors: SJ
authors: Chang
authors: Yau-Huei
authors: YH
authors: Wei
authors: Chii-Ruey
authors: CR
authors: Tzeng
mesh_terms: D004272:DNA, Mitochondrial / Q000235:genetics*; D004622:Embryo, Mammalian / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D015321:Gene Rearrangement; D006801:Humans; D009865:Oocytes / Q000502:physiology*; D011446:Prospective Studies
chemical_list: D004272:DNA, Mitochondrial