Detection of highly pathogenic and low pathogenic avian influenza subtype H5 (Eurasian lineage) using NASBA.
Nucleic acid sequence-based amplification (NASBA) is a technique that allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique has been developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The NASBA technique described below is rapid and specific for the identification of influenza A subtype H5 viruses of the Eurasian lineage. More importantly, it can be used to distinguish highly pathogenic and low pathogenic strains of the H5 subtype.
Collins
Richard A
RA
Hong Kong DNA Chips Ltd, 1805-6, 18/F, Lu Plaza, 2 Wing Yip Street, Hong Kong SAR, Kowloon, China.
Ko
Lung Sang
LS
So
Ka Lun
KL
Ellis
Trevor
T
Lau
Lok Ting
LT
Yu
Albert Cheung Hoi
AC
D000818:Animals; D001717:Birds; D002642:Chick Embryo; D017931:DNA Primers; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000175:diagnosis / Q000821:virology; D008163:Luminescent Measurements; D021141:Nucleic Acid Amplification Techniques; D012367:RNA, Viral / Q000032:analysis; D013552:Swine
D017931:DNA Primers; D012367:RNA, Viral