title: Anosmin-1, defective in the X-linked form of Kallmann syndrome, promotes axonal branch formation from olfactory bulb output neurons.
abstract: The physiological role of anosmin-1, defective in the X chromosome-linked form of Kallmann syndrome, is not yet known. Here, we show that anti-anosmin-1 antibodies block the formation of the collateral branches of rat olfactory bulb output neurons (mitral and tufted cells) in organotypic cultures. Moreover, anosmin-1 greatly enhances axonal branching of these dissociated neurons in culture. In addition, coculture experiments with either piriform cortex or anosmin-1-producing CHO cells demonstrate that anosmin-1 is a chemoattractant for the axons of these neurons, suggesting that this protein, which is expressed in the piriform cortex, attracts their collateral branches in vivo. We conclude that anosmin-1 has a dual branch-promoting and guidance activity, which plays an essential role in the patterning of mitral and tufted cell axon collaterals to the olfactory cortex.
authors: Unité de Génétique des Déficits Sensoriels, Centre National de la Recherche Scientifique, Unité de Recherche Associée 1968, Institut Pasteur, 25 rue du Dr Roux, F-75724 Paris cedex 15, France. nyani@pasteur.fr
authors: Nadia
authors: N
authors: Soussi-Yanicostas
authors: Fernando
authors: F
authors: de Castro
authors: A Karyn
authors: AK
authors: Julliard
authors: Isabelle
authors: I
authors: Perfettini
authors: Alain
authors: A
authors: Chédotal
authors: Christine
authors: C
authors: Petit
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D019521:Body Patterning / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002630:Chemotactic Factors / Q000235:genetics / Q000378:metabolism; D002633:Chemotaxis / Q000502:physiology*; D006224:Cricetinae; D016326:Extracellular Matrix Proteins; D005260:Female; D005333:Fetus; D005727:Ganglia, Spinal / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D017436:Kallmann Syndrome / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D009415:Nerve Net / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009830:Olfactory Bulb / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D009833:Olfactory Pathways / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D014960:X Chromosome / Q000235:genetics*
chemical_list: C076266:ANOS1 protein, human; D002630:Chemotactic Factors; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins