Spontaneous secretion of immunoglobulins and anti-HIV-1 antibodies by in vivo activated B lymphocytes from HIV-1-infected subjects: monocyte and natural killer cell requirement for in vitro terminal differentiation into plasma cells.
Peripheral blood mononuclear cells from HIV-1-infected subjects secrete spontaneously in vitro immunoglobulins (Ig) and anti-HIV-1 antibodies (Ab). Purified B lymphocytes secrete only minute amounts of Ig and anti-HIV-1 Ab compared with unfractionated cells. Monocytes and natural killer cells enhanced both secretions by cell-to-cell contacts, involving adhesion and CD27, CD80 costimulatory molecules and IL-6. Cell interactions prolonged the survival and allowed the terminal maturation of in vivo activated B cells. The secreting cell precursors were highly differentiated B cells expressing a broad diversity of maturation markers (CD27(+), CD38(+), CD20(+/-), CD37(+/-), CD71(+/-), HLA-DQ(+/-), sIg(+/-)) but not sIgD, CD28, or CD40. This phenotype and the cytologic aspect of purified B cells suggest that these cells are early plasma cells originated from germinal center. Ex vivo secreting peripheral B cells had probably gone beyond the CD40/CD40 ligand interaction; then following CD28/CD80 and CD27/CD27 ligand (CD70) interactions in the presence of IL-6, they achieved in vitro their differentiation into plasma cells.
Fournier
Anne Marie
AM
Département de Virologie Clinique, Centre Hospitalier et Universitaire, Montpellier, France.
Fondere
Jean-Michel
JM
Alix-Panabieres
Catherine
C
Merle
Corinne
C
Baillat
Vincent
V
Huguet
Marie-France
MF
Taïb
Jacques
J
Ohayon
Viviane
V
Zembala
Marek
M
Reynes
Jacques
J
Vendrell
Jean Pierre
JP
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology*; D019013:CD40 Antigens / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000502:physiology; D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D015483:HIV Antibodies / Q000096:biosynthesis*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000502:physiology*; D008213:Lymphocyte Activation; D009000:Monocytes / Q000502:physiology*; D010950:Plasma Cells / Q000502:physiology*; D011990:Receptors, Transferrin
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D019013:CD40 Antigens; C068126:CD71 antigen; D015815:Cell Adhesion Molecules; D015483:HIV Antibodies; D007136:Immunoglobulins; D015850:Interleukin-6; D011990:Receptors, Transferrin