title: The delta subunit of AP-3 is required for efficient transport of VSV-G from the trans-Golgi network to the cell surface.
abstract: Vesicular stomatitis virus glycoprotein (VSV-G) is a transmembrane protein that functions as the surface coat of enveloped viral particles. We report the surprising result that VSV-G uses the tyrosine-based di-acidic motif (-YTDIE-) found in its cytoplasmic tail to recruit adaptor protein complex 3 for export from the trans-Golgi network. The same sorting code is used to recruit coat complex II to direct efficient transport from the endoplasmic reticulum to the Golgi apparatus. These results demonstrate that a single sorting sequence can interact with sequential coat machineries to direct transport through the secretory pathway. We propose that use of this compact sorting domain reflects a need for both efficient endoplasmic reticulum export and concentration of VSV-G into specialized post-trans-Golgi network secretory-lysosome type transport containers to facilitate formation of viral coats at the cell surface.
authors: Department of Cell Biology and the Institute for Childhood and Neglected Diseases, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors: Noriyuki
authors: N
authors: Nishimura
authors: Helen
authors: H
authors: Plutner
authors: Klaus
authors: K
authors: Hahn
authors: William E
authors: WE
authors: Balch
mesh_terms: D033963:Adaptor Protein Complex 3; D033966:Adaptor Protein Complex beta Subunits; D033981:Adaptor Protein Complex delta Subunits; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001665:Binding Sites; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D014157:Transcription Factors / Q000378:metabolism*; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D021601:trans-Golgi Network / Q000378:metabolism*
chemical_list: C491904:AP3D1 protein, human; D033963:Adaptor Protein Complex 3; D033966:Adaptor Protein Complex beta Subunits; D033981:Adaptor Protein Complex delta Subunits; D015703:Antigens, CD; C113910:Ap3d1 protein, mouse; C028391:G protein, vesicular stomatitis virus; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D014157:Transcription Factors; D014759:Viral Envelope Proteins