Identifying the major proteome components of Helicobacter pylori strain 26695.
The whole genome sequences of Helicobacter pylori strain 26695 have been reported. Whole cell proteins of H. pylori strain 26695 cells were obtained and analyzed by two-dimensional electrophoresis, using immobilized pH gradient strips. The most abundant proteins were shown in the region of pI 4.0-9.5 with molecular masses from 10 to 100 kDa. Soluble proteins were precipitated by the use of 0-80% saturated solutions of ammonium sulfate. Soluble proteins precipitated by the 0-40% saturations of ammonium sulfate produced similar spot profiles and their abundant protein spots had acidic pI regions. However, a number of soluble proteins precipitated by more than 60% saturation of ammonium sulfate were placed in the alkaline pI regions, compared to those precipitated by 40% saturation. In addition, we have performed an extensive proteome analysis of the strain utilizing peptide MALDI-TOF-MS. Among the 345 protein spots processed, 175 proteins were identified. The identified spots represented 137 genes. One-hundred and fifteen proteins were newly identified in this study, including DNA polymerase III beta-subunit. These results might provide guidance for the enrichment of H. pylori proteins and contribute to construct a master protein map of H. pylori.
Cho
Myung-Je
MJ
Department of Microbiology, College of Medicine, Gyeongsang National University, Chinju, Kyung-Nam, Korea.
Jeon
Beong-Sam
BS
Park
Jeong-Won
JW
Jung
Tae-Sung
TS
Song
Jae-Young
JY
Lee
Woo-Kon
WK
Choi
Yeo-Jeong
YJ
Choi
Sang-Haeng
SH
Park
Seong-Gyu
SG
Park
Jeong-Uck
JU
Choe
Mi-Young
MY
Jung
Seun-Ae
SA
Byun
Eun-Young
EY
Baik
Seung-Chul
SC
Youn
Hee-Shang
HS
Ko
Gyung-Hyuck
GH
Lim
DongBin
D
Rhee
Kwang-Ho
KH
D001426:Bacterial Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D016480:Helicobacter pylori / Q000737:chemistry*; D008970:Molecular Weight; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D020543:Proteome