Development and applications of transgenesis in the yellow fever mosquito, Aedes aegypti.
Transgenesis technology has been developed for the yellow fever mosquito, Aedes aegypti. Successful integration of exogenous DNA into the germline of this mosquito has been achieved with the class II transposable elements, Hermes, mariner and piggyBac. A number of marker genes, including the cinnabar(+) gene of Drosophila melanogaster, and fluorescent protein genes, can be used to monitor the insertion of these elements. The availability of multiple elements and marker genes provides a powerful set of tools to investigate basic biological properties of this vector insect, as well as the materials for developing novel, genetics-based, control strategies for the transmission of disease.
Adelman
Zachary N
ZN
Department of Molecular Biology and Biochemistry, University of California, 3205 McGaugh, Irvine, CA 92697-3900, USA.
Jasinskiene
Nijole
N
James
Anthony A
AA
D000330:Aedes / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D004251:DNA Transposable Elements; D005819:Genetic Markers; D006801:Humans; D007303:Insect Vectors / Q000235:genetics*; D009032:Mosquito Control / Q000379:methods; D016254:Mutagenesis, Insertional; D015004:Yellow Fever / Q000517:prevention & control* / Q000635:transmission*
D004251:DNA Transposable Elements; D005819:Genetic Markers