Isolation and identification of Mycobacterium kansasii from pleural fluid of a dog with persistent pleural effusion.
A 3-year-old spayed female Whippet was examined for cough and respiratory distress. Lung lobe torsion with pleural effusion was diagnosed, and lung lobectomy was performed. Pleural effusion recurred during the following 27 months; conventional bacteriologic cultures of pleural effusion did not result in bacterial growth. A second lung lobectomy, pleuroperitoneal shunt placement. and pericardectomy were subsequently performed. Mycobacterium kansasii was eventually isolated from pleural fluid and identified by polymerase chain reaction amplification and DNA sequencing. The dog was euthanatized before therapeutic response could be evaluated. To our knowledge, this is the first report of M. kansasii infection in a dog. Additionally, this is the first report of mycobacterial isolation from pleural fluid, and one of few reports of antemortem mycobacterial isolation from a body fluid, as opposed to identification in specimens during histologic examination. Routine bacteriologic culture methods are insufficient to isolate mycobacterial agents, and special methods are indicated in dogs with persistent pleural effusion.
Pressler
Barrak M
BM
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
Hardie
Elizabeth M
EM
Pitulle
Christian
C
Hopwood
Robin M
RM
Sontakke
Sushama
S
Breitschwerdt
Edward B
EB
D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D004283:Dog Diseases / Q000175:diagnosis / Q000382:microbiology*; D004285:Dogs; D017809:Fatal Outcome; D005260:Female; D008168:Lung / Q000601:surgery; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D019909:Mycobacterium kansasii / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010996:Pleural Effusion / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012008:Recurrence
D004269:DNA, Bacterial