Isolation (from a basal cell carcinoma) of a functionally distinct fibroblast-like cell type that overexpresses Ptch.
In this study we report on the isolation and characterization of a nonepithelial, nontumorigenic cell type (BCC1) derived from a basal cell carcinoma from a patient. The BCC1 cells share many characteristics with dermal fibroblasts, such as the expression of vimentin, lack of expression of cytokeratins, and insensitivity to agents that cause growth inhibition and differentiation of epithelial cells; however, significant differences between BCC1 cells and fibroblasts also exist. For example, BCC1 cells are stimulated to undergo DNA synthesis in response to interferon-gamma, whereas dermal fibroblasts are not. More over, BCC1 cells overexpress the basal cell carcinoma-specific genes ptch and ptch2. These data indicate that basal cell carcinomas are associated with a functionally distinct population of fibroblast-like cells that overexpress known tumor-specific markers (ptch and ptch2).
Dicker
Anthony J
AJ
Epithelial Pathobiology Group, Center for Immunology & Cancer Research, University of Queensland, Princess Alexandra Hospital, Brisbane, Queensland, Australia.
Serewko
Magdalena M
MM
Russell
Terry
T
Rothnagel
Joseph A
JA
Strutton
Geoff M
GM
Dahler
Alison L
AL
Saunders
Nicholas A
NA
D014408:Biomarkers, Tumor; D015197:Carcinogenicity Tests; D002280:Basal Cell Carcinoma; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007668:Kidney / Q000166:cytology; D008565:Membrane Proteins / Q000235:genetics*; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D000072082:Patched-2 Receptor; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface; D012878:Skin Neoplasms; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D008565:Membrane Proteins; C000606236:PTCH1 protein, human; C000606238:PTCH2 protein, human; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D000072082:Patched-2 Receptor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface