Method of producing tissue sections from endometrial scrapings.
Endometrial cytology is important not only for observing cytological findings, but also for assessing histological architecture. Therefore, we attempted to produce a histological preparation from the sample using an automatic fixation apparatus (ACF 1000) which employs a membrane filter method. After observation of the cytological features, the cover slip was removed and a paraffin-embedded section was prepared. Producing a histological section from a cytological specimen prepared with the ACF 1000 apparatus was more useful and easier than with the techniques described to date. Peelability of the cell was avoided by using a silane-coated membrane filter. Observation of the same cell cluster as that observed in the cytological sample was also facilitated in the histological section by this technique.
Ono
M
M
Department of Cytology, Kyorin University School of Health Sciences, Hachioji, Tokyo, Japan.
Shiina
Y
Y
Sakuma
K
K
Inoue
H
H
D001706:Biopsy; D004717:Endometrium / Q000473:pathology*; D005260:Female; D016591:Histocytological Preparation Techniques / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D013048:Specimen Handling / Q000379:methods; D016707:Tissue Fixation / Q000295:instrumentation / Q000379:methods; D014462:Ultrafiltration