title: Analysis of heat-shock related gene expression in head-and-neck cancer using cDNA arrays.
abstract: OBJECTIVE
  The aim of the present study was to identify genes regulated in the early response to heat-shock in human head-and-neck cancer cells using a cDNA array.
  
  
  METHODS
  IMC-3 cells were heat-shocked at 44 degrees C for 30 min, then incubated for 6 h. After 6 h incubation, mRNAs were extracted. Early gene expressions in IMC-3 cells were analyzed using a cDNA array after heat-shock. In addition, reverse transcription-polymerase chain reaction (RT-PCR) was performed to identify the expressions of mRNA for confirming features by cDNA array analysis in several other cell lines (KB, T3M-1, SCC-TF) as well as in IMC-3 cells.
  
  
  RESULTS
  Twenty-eight genes were found to be induced or upregulated by heat-shock in IMC-3 cells. These included genes involved in the apoptosis (e.g., CC3, caspase10), tumor invasion (e.g., CC3, TIMP-3), cell cycle checkpoint control (e.g., DP-1, CDC25A), signal transduction (e.g., MEK1) as well as genes associated with heat stress (e.g., Hsp70B', Hsp40). Gene expressions of CC3 and MEK1 were recognized to be induced by heat-shock in pharyngeal cancer cells (KB, T3M-1) and lingual cancer cells (SCC-TF).
  
  
  CONCLUSIONS
  The observed alteration in gene expression may play a role in various biochemical pathways of cancer cells exposed to heat-shock.
authors: Department of Otorhinolaryngology, Fukui Medical University, Matsuoka, Fukui 910-1193, Japan. norihiko@fmsrsa.fukui-med.ac.jp
authors: Norihiko
authors: N
authors: Narita
authors: Ichiro
authors: I
authors: Noda
authors: Toshio
authors: T
authors: Ohtsubo
authors: Shigeharu
authors: S
authors: Fujieda
authors: Masaharu
authors: M
authors: Tokuriki
authors: Takehisa
authors: T
authors: Saito
authors: Hitoshi
authors: H
authors: Saito
mesh_terms: D000123:Acetyltransferases; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000628:therapy; D005334:Fever; D020869:Gene Expression Profiling / Q000379:methods*; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000628:therapy; D006801:Humans; D048369:MAP Kinase Kinase 1; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D014157:Transcription Factors; D000123:Acetyltransferases; C111246:HTATIP2 protein, human; D017346:Protein Serine-Threonine Kinases; D048369:MAP Kinase Kinase 1; C482125:MAP2K1 protein, human; D020929:Mitogen-Activated Protein Kinase Kinases