Monoaminergic and peptidergic axonal projections to the vagal motor cell column of a teleost, the filefish Stephanolepis cirrhifer.
In an immunohistochemical study, the vagal motor nucleus of a teleost, the filefish Stephanolepis cirrhifer, could be divided into a rostral part and a caudal part, and the former into a dorsolateral group and a ventromedial group. The dorsolateral group consisted of neurons immunoreactive for calcitonin gene-related peptide, whereas the ventrolateral-caudal group was negative for calcitonin gene-related peptide. The latter group was retrogradely labeled after dextran amine injection to the visceral ramus of the vagus nerve, suggesting that it is a general visceral efferent column, made up of parasympathetic preganglionic neurons, whereas the dorsolateral rostral group is a special visceral efferent column. In the general visceral efferent column, a dense concentration of nerve fibers immunoreactive for serotonin, tyrosine hydroxylase, cholecystokinin-8, and substance P, and a small number of fibers immunoreactive for neuropeptide Y was observed. Perikarya in contact with varicose terminals immunoreactive for these substances were frequently seen. In contrast, in the special visceral efferent column, only a moderate concentration of neuropeptide Y-immunoreactive nerve fibers and a sparse distribution of fibers immunoreactive for tyrosine hydroxylase were observed. Perikarya in contact with varicose terminals immunoreactive for these substances were rare. These results suggest that the vagal parasympathetic preganglionic neurons might receive multiple inputs of monoaminergic and peptidergic fibers involved in the regulation of the visceral organs. On the other hand, monoaminergic and peptidergic afferent fibers might be of much less significance in the activity of the special visceral efferent component of the vagus nerve.
Funakoshi
Kengo
K
Department of Anatomy, Yokohama City University School of Medicine, Yokohama 236-0004, Japan. funako@yokohama-cu.ac.jp
Atobe
Yoshitoshi
Y
Nakano
Masato
M
Hisajima
Tatsuya
T
Goris
Richard C
RC
Kishida
Reiji
R
D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D015306:Biogenic Monoamines / Q000378:metabolism*; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism; D002766:Cholecystokinin / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003911:Dextrans; D005399:Fishes / Q000033:anatomy & histology / Q000378:metabolism*; D019793:Fluorescein; D008526:Medulla Oblongata / Q000166:cytology / Q000378:metabolism*; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D012701:Serotonin / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000166:cytology / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014630:Vagus Nerve / Q000166:cytology / Q000378:metabolism*; D014781:Viscera / Q000294:innervation / Q000502:physiology
D015306:Biogenic Monoamines; D002395:Catecholamines; D003911:Dextrans; D009478:Neuropeptide Y; D009479:Neuropeptides; D010446:Peptide Fragments; C410885:cholecystokinin 8; D012701:Serotonin; D013373:Substance P; D002766:Cholecystokinin; D014446:Tyrosine 3-Monooxygenase; D002795:Choline O-Acetyltransferase; D015740:Calcitonin Gene-Related Peptide; D019793:Fluorescein