Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose.
Evidence is presented that the specificity of jacalin, the seed lectin from jack fruit (Artocarpus integrifolia), is not directed exclusively against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and N-acetylmuramic acid. Structural analyses demonstrated that the relatively large size of the carbohydrate-binding site enables jacalin to accommodate monosaccharides with different hydroxyl conformations and provided unambiguous evidence that the beta-prism structure of jacalin is a sufficiently flexible structural scaffold to confer different carbohydrate-binding specificities to a single lectin.
Bourne
Yves
Y
AFMB, UMR-CNRS 6098, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
Astoul
Corinne Houlès
CH
Zamboni
Véronique
V
Peumans
Willy J
WJ
Menu-Bouaouiche
Laurence
L
Van Damme
Els J M
EJ
Barre
Annick
A
Rougé
Pierre
P
D000276:Adjuvants, Immunologic / Q000737:chemistry; D001212:Asialoglycoproteins / Q000737:chemistry; D001665:Binding Sites; D002241:Carbohydrates / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004305:Dose-Response Relationship, Drug; D060748:Fetuins; D005690:Galactose / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D008358:Mannose / Q000737:chemistry*; D008958:Models, Molecular; D009112:Muramic Acids / Q000737:chemistry; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry; D011485:Protein Binding; D020349:Surface Plasmon Resonance; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000737:chemistry
D000276:Adjuvants, Immunologic; D001212:Asialoglycoproteins; D002241:Carbohydrates; D060748:Fetuins; D006023:Glycoproteins; D037102:Lectins; D009112:Muramic Acids; D037121:Plant Lectins; D010940:Plant Proteins; D000509:alpha-Fetoproteins; C110154:arcelin-1; C017029:asialofetuin; C043696:jacalin; C031651:N-acetylmuramic acid; D019158:N-Acetylneuraminic Acid; D005947:Glucose; D008358:Mannose; D005690:Galactose