Stereologic characteristics of pig small intestine during normal development.
Stereologic methods were used to study the behavior of the pig's intestinal wall during periods that are characterized by a high incidence of gastrointestinal disorders. For this purpose conventionally stained transverse and vertical paraffin sections were made of the small intestine (duodenum, jejunum, and ileum) of fetal, neonatal, and weaned pigs. The volumes of the intestinal walls were estimated using Cavalieri's method. Subsequently, the surface density (Sv) of the tunica mucosa and the volume densities (Vv) of the different small intestinal elements were estimated. Finally, the surface and volumes per serosal surface area (Ss and Vs) were calculated. The decrease of Sv can be attributed to the finding that the mucosal surface increases to a lesser extent compared with the volume of the intestinal wall. The Vs of the various layers increased postnatally, illustrating that the intestinal wall thickens. Despite an increasing total mucosal surface, this postnatal thickening causes Ss to decline. Each of these changes is temporally related to dietary changes, an increased antigen load, and an increased need for protection. Additionally, the regional differences of the various parameters match the qualitative descriptions of the small intestine of the pig and relate to region-specific functions.
Van Ginneken
C
C
Laboratory of Veterinary Anatomy and Embryology, University of Antwerp, Belgium.
Van Meir
F
F
Weyns
A
A
D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology / Q000254:growth & development*; D002011:Brunner Glands / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development; D005314:Embryonic and Fetal Development; D005333:Fetus / Q000033:anatomy & histology; D007421:Intestine, Small / Q000033:anatomy & histology / Q000196:embryology* / Q000254:growth & development*; D012016:Reference Values; D012704:Serous Membrane / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development; D013552:Swine