[Quantification of metallothionein induced with selenium by using competitive ELISA].
Metallothionein induced by selenium was assayed with competitive ELISA. It was found that in control mice, the liver contained (2.47+/-0.90) microgram MT/g wet weight tissue, while the mice administered with ZnSO(4) contained (8.15+/-2.20) microgram MT/g wet weight liver tissue and the mice given with selenium malt contained (12.80+/-1.44) microgram MT/g wet weight liver. A significant difference was found between the control and the latter two groups (P<0.05, P<0.05). The MT content of selenium group was significantly higher than the zinc group. It is suggested that the selenium was more potent in induction of MT and was a stronger inducer than ZnSO(4) in its effective dose range, therefore selenium may be developed into a novel and safe MT inducer.
Liu
Ying
Y
National Laboratory of Protein Engineering, College of Life Sciences, Peking University, Beijing 100871, China. rulab@pku.edu.cn
Jiang
Guan-Qun
GQ
Ru
Bing-Gem
BG
Xu
Zhuo-Li
ZL
Guo
Jun-Hua
JH
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D011817:Rabbits; D012643:Selenium / Q000494:pharmacology*; D019287:Zinc Sulfate / Q000494:pharmacology
D019287:Zinc Sulfate; D008668:Metallothionein; D012643:Selenium