Relationship between interleukin-1-alpha concentration in tumors and cell growth in gastric cancer, determined using flow cytometry.
BACKGROUND: Interleukin-1-alpha (IL-1alpha) produced by tumor cells stimulates the proliferation or the growth of several cancer cell lines. The aim of this study was to clarify the relationship between growth activity evaluated by DNA analysis and the concentration of tumor-derived IL-1alpha in gastric cancers in clinical cases.METHODS: We measured the concentration of IL-1alpha in homogenized tumor samples obtained from 49 patients with gastric cancer, using an enzyme-linked immunosorbent assay, and we analyzed the cellular DNA content of paraffin-embedded tumor sections using flow cytometry.RESULTS: Both the IL-1alpha concentration and the percentage of S-phase fraction were significantly correlated with liver metastasis, histologic type, pattern of tumor infiltration, quantity of stroma, and venous invasion. A good correlation was found between IL-1alpha concentration in tumors and the percentage of S-phase fraction ( R = 0.604, P < 0.0001).CONCLUSION: IL-1alpha may be related to the stimulation of cell growth of gastric cancer in clinical cases.
Furuya
Yoshitaka
Y
First Department of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
Ichikura
Takashi
T
Mochizuki
Hidetaka
H
Shinomiya
Nariyoshi
N