Isolation and identification of a new bifidogenic growth stimulator produced by Propionibacterium freudenreichii ET-3.
We have found a new growth stimulator for bifidobacteria in the culture broth of Propionibacterium freudenreichii ET-3. The bifidogenic growth stimulator (BGS) was purified by Diaion HP-20 column chromatography and preparative HPLC. Spectroscopic methods including 1H-NMR, UV, and LC-ESI-MS experiments indicated that the chemical structure of the bifidogenic growth stimulator was 1,4-dihydroxy-2-naphthoic acid (DHNA). Approximately 10 mg/L of DHNA was found to be produced in the culture broth of P. freudenreichii ET-3.
Isawa
Kakuhei
K
Research & Development Center, Meiji Dairies Corporation, Kanagawa, Japan.
Hojo
Kenichi
K
Yoda
Nobuo
N
Kamiyama
Tomonori
T
Makino
Seiya
S
Saito
Mizue
M
Sugano
Hitomi
H
Mizoguchi
Chinami
C
Kurama
Saori
S
Shibasaki
Mika
M
Endo
Noriko
N
Sato
Yoshio
Y
D001644:Bifidobacterium / Q000187:drug effects* / Q000254:growth & development*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D009284:Naphthols / Q000378:metabolism* / Q000494:pharmacology*; D011424:Propionibacterium / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D009284:Naphthols; C024023:1,4-dihydroxy-2-naphthoic acid