title: Enterocin 416K1, an antilisterial bacteriocin produced by Enterococcus casseliflavus IM 416K1 isolated from Italian sausages.
abstract: Enterococci (118) from Italian sausages were tested for the production of antimicrobial substances. Of these, 7.6% showed antibacterial activity against one or several closely related microorganisms used as indicators. Enterococcus casseliflavus IM 416K1 in particular produced a bacteriocin (Enterocin 416K1) with strong anti-listerial antagonistic activity. The bacteriocin withstood heating at 90 degrees C for 120 min and storage at 4 degrees C for 6 months. The mode of action was identified as bactericidal. The crude activity of Enterocin 416K1 was linked to a molecule with an apparent molecular weight smaller than 5 kDa. Plasmid analysis of E. casseliflavus IM 416K1 revealed the presence of four plasmids with different molecular weights (34, 11, 7 and 3.3 MDa). All the Bac- variants produced by curing experiments showed loss of the single plasmid of 34 MDa. Bacteriocin activity and immunity production may be linked to genes located on that same plasmid.
authors: Department of Biomedical Sciences, University of Modena and Reggio E., Modena, Italy.
authors: C
authors: C
authors: Sabia
authors: G
authors: G
authors: Manicardi
authors: P
authors: P
authors: Messi
authors: S
authors: S
authors: de Niederhäusern
authors: M
authors: M
authors: Bondi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D001952:Bridged-Ring Compounds / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D003257:Consumer Product Safety; D016983:Enterococcus / Q000378:metabolism*; D005516:Food Microbiology; D006801:Humans; D007700:Kinetics; D008461:Meat Products / Q000032:analysis* / Q000382:microbiology; D008970:Molecular Weight; D010957:Plasmids
chemical_list: D000900:Anti-Bacterial Agents; D001952:Bridged-Ring Compounds; C012306:enterocin