title: Human erythroid cells produced ex vivo at large scale differentiate into red blood cells in vivo.
abstract: New sources of red blood cells (RBCs) would improve the transfusion capacity of blood centers. Our objective was to generate cells for transfusion by inducing a massive proliferation of hematopoietic stem and progenitor cells, followed by terminal erythroid differentiation. We describe here a procedure for amplifying hematopoietic stem cells (HSCs) from human cord blood (CB) by the sequential application of specific combinations of growth factors in a serum-free culture medium. The procedure allowed the ex vivo expansion of CD34+ progenitor and stem cells into a pure erythroid precursor population. When injected into nonobese diabetic, severe combined immunodeficient (NOD/SCID) mice, the erythroid cells were capable of proliferation and terminal differentiation into mature enucleated RBCs. The approach may eventually be useful in clinical transfusion applications.
authors: Institut National de la Santé et de la Recherche Médicale U417, Hôpital Saint-Antoine, Paris, France.
authors: Thi My Anh
authors: TM
authors: Neildez-Nguyen
authors: Henri
authors: H
authors: Wajcman
authors: Michael C
authors: MC
authors: Marden
authors: Morad
authors: M
authors: Bensidhoum
authors: Vincent
authors: V
authors: Moncollin
authors: Marie-Catherine
authors: MC
authors: Giarratana
authors: Ladan
authors: L
authors: Kobari
authors: Dominique
authors: D
authors: Thierry
authors: Luc
authors: L
authors: Douay
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis; D002454:Cell Differentiation; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology*; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D051379:Mice; D016513:Mice, SCID; D010477:Perfusion; D013234:Stem Cells / Q000378:metabolism; D013997:Time Factors
chemical_list: D018952:Antigens, CD34; D016895:Culture Media, Serum-Free; D006454:Hemoglobins