New approaches in the development of DNA sensors: hybridization and electrochemical detection of DNA and RNA at two different surfaces.
Up to now, the development of the electrochemical DNA hybridization sensors relied on solid electrodes, on which both the hybridization and detection steps have been performed. Here we propose a new method in which the DNA hybridization is performed at commercially available magnetic beads and electrochemical detection on detection electrodes (DE). Due to minimum nonspecific DNA adsorption at the magnetic beads, very high specificity of the DNA hybridization is achieved. Optimum DE can be chosen only with respect to the given electrode process. It is shown that high sensitivity and specificity in the detection of relatively long target DNAs can be obtained (a) by using cathodic stripping voltammetry at mercury or solid mercury amalgam DEs for the determination of purine bases, released from DNA by acid treatment, and (b) by enzyme-linked immunoassay of target DNA modified by osmium tetroxide,2,2'-bipyridine (Os,bipy) at carbon DEs. Direct determination of Os,bipy at mercury and carbon electrodes is also possible.
Palecek
E
E
Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolska 135, 61265 Brno, Czech Republic. palecek@ibp.cz
Fojta
M
M
Jelen
F
F
D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000737:chemistry*; D012680:Sensitivity and Specificity
D012313:RNA; D004247:DNA