The SAM domain of polyhomeotic forms a helical polymer.
The polycomb group (PcG) proteins are important in the maintenance of stable repression patterns during development. Several PcG members contain a protein protein interaction module called a SAM domain (also known as SPM, PNT and HLH). Here we report the high-resolution structure of the SAM domain of polyhomeotic (Ph). Ph-SAM forms a helical polymer structure, providing a likely mechanism for the extension of PcG complexes. The structure of the polymer resembles that formed by the SAM domain of another transcriptional repressor, TEL. The formation of these polymer structures by SAM domains in two divergent repressors suggests a conserved mode of repression involving a higher order chromatin structure.
Kim
Chongwoo A
CA
Department of Chemistry and Biochemistry, Laboratory of Structural Biology and Molecular Medicine, Molecular Biology Institute, UCLA, 611 Charles E. Young Drive East, Los Angeles, California 90095-1570, USA.
Gingery
Mari
M
Pilpa
Rosemarie M
RM
Bowie
James U
JU
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001704:Biopolymers / Q000737:chemistry / Q000235:genetics; D002843:Chromatin / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure*; D019281:Dimerization; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D019143:Evolution, Molecular; D008854:Microscopy, Electron; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D009698:Nucleoproteins / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure*; D063150:Polycomb Repressive Complex 1; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D016415:Sequence Alignment; D013329:Structure-Activity Relationship; D020349:Surface Plasmon Resonance
D001704:Biopolymers; D002843:Chromatin; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009698:Nucleoproteins; D011993:Recombinant Fusion Proteins; C072820:ph-d protein, Drosophila; D063150:Polycomb Repressive Complex 1