Gelling properties of heat-denatured beta-lactoglobulin aggregates in a high-salt buffer.
Thermal denaturation, rheological, and microstructural properties of gels prepared from native beta-lactoglobulin (beta-LG) and preheated or heat-denatured beta-LG (HDLG) aggregates were compared. The HDLG was prepared by heating solutions of 4% beta-LG in deionized water, pH 7.0, at 80 degrees C for 30 min and then diluted to the desired concentration in 0.6 M NaCl and 0.05 M phosphate buffer at pH 6.0, 6.5, and 7.0. When reheated to 71 degrees C, HDLG formed a gel at a concentration of 2% protein. At pH 7.0, 3% HDLG gelled at 52.5 degrees C and had a storage modulus (G') of 2200 Pa after cooling. beta-LG (3%) in 0.6 M NaCl and 0.05 M phosphate buffer, pH 7.0, did not gel when heated to 71 degrees C. The gel point of 3% HDLG decreased by 10.5 degrees C and the G' did not change when the pH was decreased to 6.0. The HDLG gel microstructure was composed of strands and clumps of small globular aggregates in contrast to beta-LG gels, which contained a particulate network of compacted globules. The HDLG formed a gel at a lower concentration and lower temperature than beta-LG in the high-salt buffer, suggesting an application in meat systems or other food products prepared with salt and processed at temperatures of < or =71 degrees C.
Vittayanont
Manee
M
Department of Food Science and Human Nutrition and Center for Advanced Microscopy, Michigan State University, East Lansing, Michigan 48824-1224, USA.
Steffe
James F
JF
Flegler
Stanley L
SL
Smith
Denise M
DM
D002021:Buffers; D002152:Calorimetry, Differential Scanning; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D005524:Food Technology; D005782:Gels; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007782:Lactoglobulins / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010710:Phosphates; D011489:Protein Denaturation; D012212:Rheology; D012965:Sodium Chloride; D012996:Solutions
D002021:Buffers; D005782:Gels; D007782:Lactoglobulins; D010710:Phosphates; D012996:Solutions; D012965:Sodium Chloride