Isolation of a cotton RGP gene: a homolog of reversibly glycosylated polypeptide highly expressed during fiber development.
A full-length cDNA encoding putative reversibly glycosylated polypeptide (RGP) was cloned from cotton fiber cells using differential display combined with rapid amplification of the cDNA ends. The gene, designated GhRGP1, contains an open reading frame of 1080 bp encoding a protein of 359 amino acids which has 78-86% identity with other plant RGPs. Northern blot analysis showed that the gene is preferentially expressed in fiber cells and its transcripts are abundant both at the primary cell wall elongation stage and at the later stage of secondary cell thickening, suggesting that GhRGP1 may be involved in non-cellulosic polysaccharide biosynthesis of the plant cell wall.
Zhao
Guang-Rong
GR
Department of Biological Sciences and Biotechnology, Tsinghua University, 100084, Beijing, PR China.
Liu
Jin-Yuan
JY
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D018628:Gene Dosage; D006023:Glycoproteins / Q000235:genetics*; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D006023:Glycoproteins; D010940:Plant Proteins; C106959:RGP1 protein, Arabidopsis; D012313:RNA