title: Chymase inhibitors may prevent postoperative adhesion formation.
abstract: OBJECTIVE
  To clarify the role of chymase produced from mast cells in adhesion formation, we measured chymase activity level and investigated the preventive effect of a chymase inhibitor, Suc-Val-Pro-Phe(p)(OPh)(2), on the postoperative adhesion formation.
  
  
  METHODS
  Prospective randomized study using a surgical model for adhesion formation.
  
  
  METHODS
  Clean hamsters in an academic research environment.
  
  
  METHODS
  Sixty-seven female Syrian hamsters.
  
  
  METHODS
  Hamsters were given a lesion, produced by uterus scraping, and the chymase inhibitor (10 microM) or placebo was injected into the abdomen. Chymase activities in uteri were measured 3 days after the operation, and the scores of adhesion formations were assessed at 2 weeks.
  
  
  METHODS
  Measurement of chymase activity and scoring of adhesion formation were performed.
  
  
  RESULTS
  A significant increase of chymase activity in the injured uterus reduced by treatment with the chymase inhibitor. The scores of adhesion formations in the chymase inhibitor-treated group were significantly decreased in comparison with those in the placebo-treated group.
  
  
  CONCLUSIONS
  Chymase contained in mast cells plays an important role in adhesion formation, and a chymase inhibitor may be a useful drug for prevention of adhesion formation.
authors: Department of Pharmacology, Osaka Medical College, Takatsuki City, Osaka, Japan.
authors: Yukiko
authors: Y
authors: Okamoto
authors: Shinji
authors: S
authors: Takai
authors: Mayumi
authors: M
authors: Yamada
authors: Mizuo
authors: M
authors: Miyazaki
mesh_terms: D000818:Animals; D053818:Chymases; D006224:Cricetinae; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D013509:Gynecologic Surgical Procedures / Q000009:adverse effects; D008647:Mesocricetus; D009842:Oligopeptides / Q000494:pharmacology*; D010532:Peritoneal Diseases / Q000473:pathology / Q000517:prevention & control; D012697:Serine Endopeptidases / Q000378:metabolism*; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control; D014599:Uterus / Q000201:enzymology / Q000601:surgery*
chemical_list: D004791:Enzyme Inhibitors; D009842:Oligopeptides; C102024:succinyl-valyl-prolyl-phenylalanyl-diphenylphosphonate; D012697:Serine Endopeptidases; D053818:Chymases