title: Effects of brefeldin A and nordihydroguaiaretic acid on endomembrane dynamics and lipid synthesis in plant cells.
abstract: Effects of brefeldin A (BFA) and nordihydroguaiaretic acid (NDGA) on endomembrane structures and lipid synthesis were compared in maize root cells and tobacco Bright Yellow-2 cells. Immunofluorescence and electron microscopy studies showed that NDGA altered the structure and distribution of the endoplasmic reticulum (ER) within 1 h but not of the Golgi apparatus whereas, as shown previously, BFA altered that organization of the Golgi apparatus and, only subsequently, of the ER. Biochemical studies revealed that both drugs and especially BFA led to a strong inhibition of the phytosterol biosynthetic pathway: BFA led to accumulation of sterol precursors. The importance of phytosterols in membrane architecture and membrane trafficking is discussed.
authors: Laboratoire de Dynamique de la Compartimentation Cellulaire, Institut des Sciences du Végétal, CNRS UPR 2355, 91198 Cedex, Gif-sur-Yvette, France.
authors: Patricia
authors: P
authors: Mérigout
authors: François
authors: F
authors: Képès
authors: Anne-Marie
authors: AM
authors: Perret
authors: Béatrice
authors: B
authors: Satiat-Jeunemaitre
authors: Patrick
authors: P
authors: Moreau
mesh_terms: D020126:Brefeldin A / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000187:drug effects / Q000648:ultrastructure; D008055:Lipids / Q000096:biosynthesis*; D009637:Masoprocol / Q000494:pharmacology*; D010840:Phytosterols / Q000096:biosynthesis; D010944:Plants / Q000187:drug effects*; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D008055:Lipids; D010840:Phytosterols; D020126:Brefeldin A; D009637:Masoprocol