title: A fine physical map of the rice chromosome 4.
abstract: As part of an international effort to completely sequence the rice genome, we have produced a fine bacterial artificial chromosome (BAC)-based physical map of the Oryza sativa japonica Nipponbare chromosome 4 through an integration of 114 sequenced BAC clones from a taxonomically related subspecies O. sativa indica Guangluai 4 and 182 RFLP and 407 expressed sequence tag (EST) markers with the fingerprinted data of the Nipponbare genome. The map consists of 11 contigs with a total length of 34.5 Mb covering 94% of the estimated chromosome size (36.8 Mb). BAC clones corresponding to telomeres, as well as to the centromere position, were determined by BAC-pachytene chromosome fluorescence in situ hybridization (FISH). This gave rise to an estimated length ratio of 5.13 for the long arm and 2.9 for the short arm (on the basis of the physical map), which indicates that the short arm is a highly condensed one. The FISH analysis and physical mapping also showed that the short arm and the pericentromeric region of the long arm are rich in heterochromatin, which occupied 45% of the chromosome, indicating that this chromosome is likely very difficult to sequence. To our knowledge, this map provides the first example of a rapid and reliable physical mapping on the basis of the integration of the data from two taxonomically related subspecies.
authors: National Center for Gene Research, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China.
authors: Qiang
authors: Q
authors: Zhao
authors: Yu
authors: Y
authors: Zhang
authors: Zhukuan
authors: Z
authors: Cheng
authors: Mingsheng
authors: M
authors: Chen
authors: Shengyue
authors: S
authors: Wang
authors: Qi
authors: Q
authors: Feng
authors: Yucheng
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authors: Huang
authors: Ying
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authors: Li
authors: Yesheng
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authors: Tang
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authors: Zhou
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authors: Chen
authors: Shuliang
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authors: Yu
authors: Jingjie
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authors: Zhu
authors: Xin
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authors: Hu
authors: Jie
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authors: Mu
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authors: Ying
authors: Pei
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authors: Hao
authors: Lei
authors: L
authors: Zhang
authors: Yiqi
authors: Y
authors: Lu
authors: Lei S
authors: LS
authors: Zhang
authors: Yilei
authors: Y
authors: Liu
authors: Zhen
authors: Z
authors: Yu
authors: Danlin
authors: D
authors: Fan
authors: Qijun
authors: Q
authors: Weng
authors: Ling
authors: L
authors: Chen
authors: Tingting
authors: T
authors: Lu
authors: Xiaohui
authors: X
authors: Liu
authors: Peixin
authors: P
authors: Jia
authors: Tongguo
authors: T
authors: Sun
authors: Yongrui
authors: Y
authors: Wu
authors: Yujun
authors: Y
authors: Zhang
authors: Ying
authors: Y
authors: Lu
authors: Can
authors: C
authors: Li
authors: Rong
authors: R
authors: Wang
authors: Haiyan
authors: H
authors: Lei
authors: Tao
authors: T
authors: Li
authors: Hao
authors: H
authors: Hu
authors: Mei
authors: M
authors: Wu
authors: Runquan
authors: R
authors: Zhang
authors: Jianping
authors: J
authors: Guan
authors: Jia
authors: J
authors: Zhu
authors: Gang
authors: G
authors: Fu
authors: Minghong
authors: M
authors: Gu
authors: Guofan
authors: G
authors: Hong
authors: Yongbiao
authors: Y
authors: Xue
authors: Rod
authors: R
authors: Wing
authors: Jiming
authors: J
authors: Jiang
authors: Bin
authors: B
authors: Han
mesh_terms: D002875:Chromosomes / Q000737:chemistry / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial; D020451:Contig Mapping; D020732:Cytogenetic Analysis / Q000379:methods; D018744:DNA, Plant / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D012275:Oryza / Q000737:chemistry / Q000235:genetics*; D020161:Physical Chromosome Mapping / Q000379:methods*; D011995:Recombination, Genetic / Q000235:genetics; D012639:Seeds / Q000235:genetics
chemical_list: D018744:DNA, Plant