The effect of adhesion molecule blockade on pulmonary reperfusion injury.
BACKGROUND
Selectins are the molecules involved in the initial adhesion of the activated neutrophil on pulmonary endothelium. We investigated the efficacy of selectin blockade in a selective (monoclonal antibody RMP-1) and nonselective (Fucoidin) manner in pulmonary reperfusion injury.


METHODS
Groups of six rat lungs were flushed with University of Wisconsin solution then stored at 4 degrees C for 4 hours. They then underwent sanguinous reperfusion for 30 minutes during which functional measures (gas exchange, pulmonary artery pressure, and airway pressure) of lung performance were made. After reperfusion we estimated their capillary filtration coefficient (Kfc units g/cm water/minute/g wet lung tissue) using a gravimetric technique. Four groups were studied: group I had no reperfusion, group II had 30 minutes of reperfusion, group III had infusion of 20 mg/kg Fucoidin before reperfusion, and group IV had infusion of 20 microg/mL RMP-1 before reperfusion.


RESULTS
Reperfusion injury was found between groups I and II by an increase in capillary filtration coefficient (1.048 +/- 0.316 to 3.063 +/- 0.466, p < 0.01). Groups III and IV had a significantly lower Kfc than group II (0.967 +/- 0.134 and 1.205 +/- 0.164, respectively, p < 0.01). There was no significant functional difference between groups II, III, and IV.


CONCLUSIONS
Reperfusion-induced hyperpermeability was ameliorated by selective (RMP-1) and nonselective (Fucoidin) selectin blockade.
Levine
Adrian J
AJ
Cardiothoracic Surgical Unit, Queen Elizabeth Hospital, Edgbaston, Birmingham, United Kingdom.
Parkes
Karen
K
Rooney
Stephen J
SJ
Bonser
Robert S
RS
D000403:Airway Resistance; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001794:Blood Pressure; D002199:Capillary Permeability / Q000187:drug effects; D066298:In Vitro Techniques; D008168:Lung / Q000098:blood supply* / Q000473:pathology; D016040:Lung Transplantation; D008297:Male; D009926:Organ Preservation; D009929:Organ Size; D019007:P-Selectin / Q000276:immunology / Q000502:physiology*; D011134:Polysaccharides / Q000494:pharmacology; D011651:Pulmonary Artery; D011659:Pulmonary Gas Exchange; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology*
D000911:Antibodies, Monoclonal; D019007:P-Selectin; D011134:Polysaccharides; C007789:fucoidan