Expression of cell surface NM23 proteins of human leukemia cell lines of various cellular lineage and differentiation stages.
Cell surface expression of NM23 protein is only observed on tumor cell lines, but not on normal cells. To examine what types of tumor cell line express the cell surface NM23 protein, we measured the cell surface NM23-H1 and NM23-H2 proteins of leukemia line cells on various cellular lineage and differentiation stages. The NM23-H1 was expressed on myeloid leukemia lines but not lymphoid lines, while NM23-H2 was only expressed on erythroleukemia lines. The complement-dependent cytolysis confirmed the expression of these proteins on the surface. Surface NM23-H1 and NM23-H2 proteins were decreased during in vitro erythroid and granulocyte differentiation. These results show that the surface expression of NM23 proteins is related to cellular lineage and differentiation stage of leukemia line cells.
Okabe-Kado
Junko
J
Saitama Cancer Center Research Institute, 818 Komuro, Ina, Kita-adachi, 362-0806, Saitama, Japan. jkado@cancer-c.pref.saitama.jp
Kasukabe
Takashi
T
Honma
Yoshio
Y
D014408:Biomarkers, Tumor / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D004912:Erythrocytes / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology*; D008214:Lymphocytes / Q000473:pathology; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism*; D022423:Myeloid Cells / Q000473:pathology; D054778:NM23 Nucleoside Diphosphate Kinases; D009363:Neoplasm Proteins / Q000378:metabolism; D009701:Nucleoside-Diphosphate Kinase; D014157:Transcription Factors / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D054778:NM23 Nucleoside Diphosphate Kinases; D009363:Neoplasm Proteins; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins