title: Utility of a multiplex PCR assay for detecting herpesvirus DNA in clinical samples.
abstract: A multiplex PCR was designed to amplify herpes simplex virus types 1 and 2, cytomegalovirus, and varicella-zoster virus DNA present in a diverse range of clinical material. The susceptibility of these viruses to in vivo inhibition by at least one antiviral drug was an important consideration in their inclusion in the multiplex detection system. An aliquot of equine herpesvirus was introduced into each specimen prior to extraction and served as an indicator of potential inhibitors of the PCR and a detector of suboptimal PCR conditions. Compared to virus isolation and immunofluorescence-based antigen detection, the multiplex assay yielded higher detection rates for all viruses represented in the assay. The turnaround time for performance of the assay was markedly reduced compared to those for the other techniques used to identify these viruses. More than 21,000 tests have been performed using the assay. Overall, the multiplex PCR enabled the detection of substantially increased numbers of herpesviruses, in some cases in specimens or anatomical sites where previously they were rarely if ever identified using traditional detection methods.
authors: Victorian Infectious Diseases Reference Laboratory (VIDRL), 10 Wreckyn Street, North Melbourne 3051, Victoria, Australia.
authors: Julian
authors: J
authors: Druce
authors: Mike
authors: M
authors: Catton
authors: Doris
authors: D
authors: Chibo
authors: Kirsty
authors: K
authors: Minerds
authors: David
authors: D
authors: Tyssen
authors: Renata
authors: R
authors: Kostecki
authors: Bill
authors: B
authors: Maskill
authors: Wendy
authors: W
authors: Leong-Shaw
authors: Marie
authors: M
authors: Gerrard
authors: Chris
authors: C
authors: Birch
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D005123:Eye / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification*; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012463:Saliva / Q000821:virology; D012867:Skin / Q000821:virology; D013048:Specimen Handling / Q000379:methods; D014764:Viral Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D014764:Viral Proteins