A human short-chain dehydrogenase/reductase gene: structure, chromosomal localization, tissue expression and subcellular localization of its product.
We have previously described the cloning of Hep27, a short-chain dehydrogenase/reductase, which is synthesized in human hepatoblastoma HepG2 cells following growth arrest induced by butyrate treatment. The present report describes the cloning, the structure and the physical and cytogenetic mapping of the gene coding for Hep27. We also show that Hep27 is synthesized in a limited number of human normal tissues and that it is localized in the nuclei and cytoplasm of HepG2 cells.
Pellegrini
Silvia
S
Department of Experimental Pathology and Medical Biotechnology, University of Pisa, Via S. Zeno 37, I-56127 Pisa, Italy.
Censini
Stefano
S
Guidotti
Silvia
S
Iacopetti
Paola
P
Rocchi
Mariano
M
Bianchi
Marco
M
Covacci
Antonello
A
Gabrielli
Franco
F
D000426:Alcohol Dehydrogenase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D000429:Alcohol Oxidoreductases; D001483:Base Sequence; D001940:Breast / Q000378:metabolism; D000074409:Carbonyl Reductase (NADPH); D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D003001:Cloning, Molecular; D003600:Cytosol / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D015723:Gene Library; D006801:Humans; D008099:Liver / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D010053:Ovary / Q000378:metabolism; D010306:Parotid Gland / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D012333:RNA, Messenger / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism; D014407:Tumor Cells, Cultured
D009687:Nuclear Proteins; D012333:RNA, Messenger; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D000074409:Carbonyl Reductase (NADPH); C095961:DHRS2 protein, human