Development of a fluorescence polarization assay for the determination of aflatoxins in grains.
Aflatoxins produced by Aspergillus flavus are commonly found in human and animal foods including grains, cereals, peanut products, sorghum, and soy seeds. Exposure to aflatoxins has been associated with carcinogenicity. This paper reports a simple, portable, and rapid fluorescence polarization (FP) assay for aflatoxin determination in grains. This immunoassay is field portable, homogeneous, and without any washing and cleaning steps. The assay is based upon the competition between free aflatoxin and an aflatoxin-fluorescein tracer for an aflatoxin-specific monoclonal antibody in solution. A series of naturally contaminated corn, sorghum, peanut butter, and peanut paste samples were analyzed by FP and compared with HPLC results. Similarly, spiked popcorn samples were analyzed by FP. FP results of naturally contaminated samples correlated well with HPLC (r (2) = 0.97). FP analysis of spiked popcorn samples (with a mixture of B(1)/B(2)/G(1)/G(2), 7/1/3/1, w/w) gave a good correlation with spiked values (r (2) = 0.99). However, FP consistently underestimated the aflatoxin contents. This was perhaps due to low cross-reactivity of the antibody used toward B(2), G(1), and G(2) aflatoxins. These results combined with the portability and simplicity of the assay suggest that the assay can be used for screening total aflatoxin in grains.
Nasir
Mohammad Sarwar
MS
Diachemix Corporation, 683 East Center Street, Grayslake, Illinois 60030, USA. m-nasir@diachemix.com
Jolley
Michael E
ME
D000348:Aflatoxins / Q000032:analysis*; D000818:Animals; D010367:Arachis / Q000737:chemistry; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D002523:Edible Grain / Q000737:chemistry*; D005454:Fluorescence Polarization / Q000379:methods*; D005506:Food Contamination; D006801:Humans; D007202:Indicators and Reagents; D012680:Sensitivity and Specificity; D003313:Zea mays / Q000737:chemistry
D000348:Aflatoxins; D007202:Indicators and Reagents