Neutrophils CD11b and fibroblasts PGE(2) are elevated in Alzheimer's disease.
To evaluate whether inflammation-like mechanisms present in the brain of Alzheimer's disease (AD) patients are reflected in the periphery, the expression of CD11b in peripheral blood neutrophils and the expression and activity of inflammatory markers in cultured skin fibroblasts were examined. We found significantly higher levels of CD11b in neutrophils from sporadic AD patients than in controls and this elevation was positively correlated with disease severity and progression rate of mental decline. Cultured skin fibroblasts from familial (FAD) and sporadic AD patients and from controls were immunopositive for both isoforms of cyclooxygenase with no differences between groups. In unstimulated culture, the production of prostaglandin-E2 in the medium was significantly higher in fibroblasts from sporadic AD and FAD patients than in controls, and this elevation was reverted by the addition of 25 microM of ibuprofen. Our findings provide further evidence of the presence of inflammatory and immuno-related markers in the periphery of AD patients and support those studies indicating the beneficial effects of anti-inflammatory therapy in AD.
Scali
Carla
C
Department of Pharmacology, University of Florence, Viale Pieraccini, 6, 50139, Italy.
Prosperi
Costanza
C
Bracco
Laura
L
Piccini
Carolina
C
Baronti
Roberto
R
Ginestroni
Andrea
A
Sorbi
Sandro
S
Pepeu
Giancarlo
G
Casamenti
Fiorella
F
D000368:Aged; D000544:Alzheimer Disease / Q000097:blood / Q000378:metabolism* / Q000523:psychology; D002478:Cells, Cultured; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000097:blood*; D008297:Male; D008565:Membrane Proteins; D009483:Neuropsychological Tests; D009504:Neutrophils / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism
D007527:Isoenzymes; D016177:Macrophage-1 Antigen; D008565:Membrane Proteins; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone