A novel, brain-specific mouse drebrin: cDNA cloning, chromosomal mapping, genomic structure, expression, and functional characterization.
Drebrin A, a major neuronal actin-binding protein, regulates the dendritic spine shapes of neurons. Here, we have cloned and characterized a novel mouse cDNA clone encoding a truncated form of drebrin A, named s-drebrin A. Analysis of the genomic organization of the mouse drebrin gene (Dbn1), which mapped to the central portion of chromosome 13, revealed that isoforms including s-drebrin A are generated by alternative splicing from a single drebrin gene. The s-drebrin A mRNA was expressed in the brain, but not in non-neuronal tissues. The s-drebrin A expression was barely detected in the embryonic brain, but was upregulated during postnatal development of the brain. Overexpression of GFP-tagged s-drebrin A in fibroblasts showed it to be associated with actin filaments and with changes in actin cytoskeleton organization. These findings suggest that s-drebrin A has a role in spine morphogenesis, possibly by competing the actin-binding activity with drebrin A.
Jin
Minghao
M
Department of Neurobiology and Behavior, Gunma University School of Medicine, 3-39-22 Showamachi, Maebashi,371-8511, Japan.
Tanaka
Satoshi
S
Sekino
Yuko
Y
Ren
Yong
Y
Yamazaki
Hiroyuki
H
Kawai-Hirai
Rika
R
Kojima
Nobuhiko
N
Shirao
Tomoaki
T
D000199:Actins / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D016466:CHO Cells; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D003433:Crosses, Genetic; D003599:Cytoskeleton / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005091:Exons; D005260:Female; D015870:Gene Expression; D005796:Genes / Q000235:genetics; D049452:Green Fluorescent Proteins; D007438:Introns; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009115:Muridae; D009479:Neuropeptides / Q000235:genetics* / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
D000199:Actins; D018076:DNA, Complementary; D008164:Luminescent Proteins; D009479:Neuropeptides; D011993:Recombinant Fusion Proteins; C053386:drebrins; D049452:Green Fluorescent Proteins