Measurement of endogenous carbon monoxide formation in biological systems.
Endogenous carbon monoxide (CO) formation has been measured in different biological systems using a variety of analytical procedures. The methods include gas chromatography-reduction gas detection, gas chromatography-mass spectroscopic detection, laser sensor-infrared absorption, UV-visible spectrophotometric measurement of CO-hemoglobin or CO-myoglobin complex, and formation of (14)CO from (14)C-heme formed following [2-(14)C]glycine administration. CO formation ranged from a low of 0.029 nmol/mg of protein/h in chorionic villi of term human placenta to a high of 0.28 nmol/mg of protein/h in rat olfactory receptor neurons in culture and rat liver perfusate.
Marks
Gerald S
GS
Department of Pharmacology and Toxicology, Queen's University, Kingston, Ontario, Canada, K7L 3N6. gsm@post.queensu.ca
Vreman
Hendrik J
HJ
McLaughlin
Brian E
BE
Brien
James F
JF
Nakatsu
Kanji
K
D000818:Animals; D002248:Carbon Monoxide / Q000378:metabolism*; D002263:Carboxyhemoglobin / Q000378:metabolism; D002824:Chorionic Villi / Q000378:metabolism; D005260:Female; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy
D002248:Carbon Monoxide; D002263:Carboxyhemoglobin; D006419:Heme Oxygenase (Decyclizing)