Investigation of the effect of high hydrostatic pressure on proteins and lipidic membranes by dynamic fluorescence spectroscopy.
Dynamic fluorescence spectroscopy brings new insight into the functional and structural changes of biological molecules under moderate and high hydrostatic pressure. The principles of time-resolved fluorescence methods are briefly described and the resulting type of information is summarized. A first set of selected applications of the use of dynamic fluorescence on pressure effects on proteins in terms of denaturation, ternary and quaternary structure, aggregation and also interaction with DNA are presented. A second set of applications is devoted to the effect of pressure and of cholesterol on lateral heterogeneity of lipidic membranes.
Tauc
Patrick
P
Laboratoire de Biotechnologie et de Pharmacologie Génétique Appliquée, UMR 8532 C.N.R.S., Ecole Normale Supérieure de Cachan, Cachan, France.
Mateo
C Reyes
CR
Brochon
Jean Claude
JC
D002784:Cholesterol / Q000032:analysis*; D006874:Hydrostatic Pressure; D008051:Lipid Bilayers / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors
D008051:Lipid Bilayers; D011506:Proteins; D002784:Cholesterol