title: TGFbeta1 effects on functional activity of porcine thyroid cells cultured in suspension.
abstract: Thyrotropin (TSH) and transforming growth factor beta 1 (TGFbeta1) have major roles in the regulation of folliculogenesis and differentiation in thyroid cells. Isolated porcine thyroid cells cultured in the presence of TSH on a plastic surface recover a follicular architecture and exhibit normal functional properties. The addition of TGFbeta1 to the culture medium induces important morphological changes and extracellular matrix remodelling. Similarly, thyroid cells lose their ability to organify iodine and their responsiveness to adenylate cyclase. The aim of this study was to analyse the influence of TGFbeta1 on the functional activity of thyrocytes in suspension culture, independent of follicle disruption. In this system, we demonstrate that TGFbeta1 inhibits expression of thyroperoxidase, NADPH oxidase activity, iodine uptake and, consequently, iodine organification. Moreover, TGFbeta1 decreases basal and TSH-stimulated cAMP production and TSH receptor expression. Taken together, these data converge to demonstrate an essential role of TGFbeta1 in the regulation of the thyroid cell function.
authors: IFR 53 Biomolécules, FRE CNRS 2260, Université de Reims, Champagne-Ardenne, France.
authors: N
authors: N
authors: Delorme
authors: C
authors: C
authors: Remond
authors: H
authors: H
authors: Sartelet
authors: E
authors: E
authors: Petitfrère
authors: C
authors: C
authors: Clément
authors: C
authors: C
authors: Schneider
authors: G
authors: G
authors: Bellon
authors: A
authors: A
authors: Virion
authors: B
authors: B
authors: Haye
authors: L
authors: L
authors: Martiny
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D007453:Iodide Peroxidase / Q000378:metabolism; D007457:Iodine Radioisotopes / Q000378:metabolism; D008854:Microscopy, Electron; D019255:NADPH Oxidases / Q000378:metabolism; D011989:Receptors, Thyrotropin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D019700:Thrombospondin 1 / Q000378:metabolism; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D013972:Thyrotropin / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1
chemical_list: D007457:Iodine Radioisotopes; D011989:Receptors, Thyrotropin; D019700:Thrombospondin 1; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D013972:Thyrotropin; D000242:Cyclic AMP; D007453:Iodide Peroxidase; D019255:NADPH Oxidases