Determination of vitamins A (retinol) and E (alpha-tocopherol) in foods by liquid chromatography: collaborative study.
A collaborative study was conducted for the determination of vitamins A and E. Existing AOAC liquid chromatographic (LC) methods are suited for specific vitamins A and E analytical applications. This method differs from existing methods in that it can be used to assay samples in all 9 sectors of the food matrix. Standards and test samples are saponified in basic ethanol-water solution, neutralized, and diluted, converting fats to fatty acids and retinol esters and tocopherol esters to retinol and tocopherol, respectively. Retinol and alpha-tocopherol are quantitated on separate LC systems, using UV detection at 313 or 328 nm for retinol, and fluorescence detection (excitation 290 nm, emission 330 nm) for alpha-tocopherol. Vitamin concentrations are calculated by comparison of the peak heights or peak areas of vitamins in test samples with those of standards.
De Vries
Jonathan W
JW
Medallion Laboratories, Minneapolis, MN 55427, USA. jon.devries@genmills.com
Silvera
Karlene R
KR
D002853:Chromatography, Liquid; D005504:Food Analysis / Q000379:methods*; D014801:Vitamin A / Q000032:analysis*; D014810:Vitamin E / Q000032:analysis*
D014801:Vitamin A; D014810:Vitamin E