Plasma and salivary IgA subclasses and IgM in HIV-1-infected individuals.
We examined IgA and IgM responses in parotid saliva from human immunodeficiency virus-1 (HIV-1)-infected individuals. Compared to the uninfected controls, levels of salivary secretory IgA2 were significantly increased in HIV-1-infected subjects, with higher levels in those who displayed oral manifestations of HIV- I infection. Assessed by enzyme immunoassay, about two thirds of the HIV-1-infected individuals tested positive for salivary HIV-1-specific IgA antibodies but not for salivary IgM. No clear correlations between the amount of HIV-1-specific IgA and CD4 counts or plasma viral loads were found. The proportions of HIV-1-specific IgA did not correlate with the levels of total IgA. Determined by Western blot, IgA1 accounted for the majority of anti-HIV-1 IgA antibodies in saliva. Comparisons between the specificities of plasma and salivary IgA directed to HIV-1 proteins revealed the absence of salivary anti-gp41 IgA antibodies, and lower HIV-1-specific reactivity of IgA and IgM were determined in saliva than in plasma.
Wu
Xueling
X
Department of Microbiology, University of Alabama at Birmingham, 35294-2170, USA. xueling@uab.edu
Jackson
Susan
S
D000328:Adult; D000368:Aged; D015153:Blotting, Western; D018791:CD4 Lymphocyte Count; D002180:Candidiasis, Oral / Q000150:complications; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D007075:Immunoglobulin M / Q000097:blood*; D017733:Leukoplakia, Hairy / Q000150:complications; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000276:immunology*
D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007075:Immunoglobulin M