Eukaryotic elongation factor 1A interacts with the upstream pseudoknot domain in the 3' untranslated region of tobacco mosaic virus RNA.
The genomic RNA of tobacco mosaic virus (TMV), like that of other positive-strand RNA viruses, acts as a template for both translation and replication. The highly structured 3' untranslated region (UTR) of TMV RNAs plays an important role in both processes; it is not polyadenylated but ends with a tRNA-like structure (TLS) preceded by a conserved upstream pseudoknot domain (UPD). The TLS of tobamoviral RNAs can be specifically aminoacylated and, in this state, can interact with eukaryotic elongation factor 1A (eEF1A)/GTP with high affinity. Using a UV cross-linking assay, we detected another specific binding site for eEF1A/GTP, within the UPDs of TMV and crucifer-infecting tobamovirus (crTMV), that does not require aminoacylation. A mutational analysis revealed that UPD pseudoknot conformation and some conserved primary sequence elements are required for this interaction. Its possible role in the regulation of tobamovirus gene expression and replication is discussed.
Zeenko
Vladimir V
VV
Friedrich Miescher Institute, CH-4002 Basel, Switzerland.
Ryabova
Lyubov A
LA
Spirin
Alexander S
AS
Rothnie
Helen M
HM
Hess
Daniel
D
Browning
Karen S
KS
Hohn
Thomas
T
D020413:3' Untranslated Regions / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D005057:Eukaryotic Cells; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020648:Peptide Elongation Factor 1 / Q000302:isolation & purification / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014026:Nicotiana; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014908:Triticum / Q000378:metabolism
D020413:3' Untranslated Regions; D020648:Peptide Elongation Factor 1; D010936:Plant Extracts; D010940:Plant Proteins; D012367:RNA, Viral