Lateral flagella of Aeromonas species are essential for epithelial cell adherence and biofilm formation.
Mesophilic Aeromonas strains express a single polar flagellum in all culture conditions and produce lateral flagella on solid media. Such hyperflagellated cells demonstrate increased adherence. Nine lateral flagella genes, lafA-U for Aeromonas hydrophila, and four Aeromonas caviae genes, lafA1, lafA2, lafB and fliU, were isolated. Mutant characterization, nucleotide and N-terminal sequencing demonstrated that the A. hydrophila and A. caviae lateral flagellins were almost identical, but were distinct from their polar flagellum counterparts. The aeromonad lateral flagellins exhibited higher molecular masses on SDS-PAGE, and this aberrant migration was thought to result from post-translational modification through glycosylation. Mutation of the Aeromonas lafB, lafS or both A. caviae lateral flagellins caused the loss of lateral flagella and a reduction in adherence and biofilm formation. Mutations in lafA1, lafA2, fliU or lafT resulted in strains that expressed lateral flagella, but had reduced adherence levels. Mutation of the lateral flagella loci did not affect polar flagellum synthesis, but the polarity of the transposon insertions on the A. hydrophila lafTlU genes resulted in non-motility. However, mutations that abolished polar flagellum production also inhibited lateral flagella expression. We conclude that Aeromonas lateral flagella: (i) play a role in adherence and biofilm formation; (ii) are distinct from the polar flagellum; (iii) synthesis is dependent upon the presence of a polar flagellum filament; and (iv) that the motor proteins of the polar and lateral flagella systems appear to be shared.
Gavín
Rosalina
R
Departamento Microbiología, Facultad Biología, Universidad Barcelona, Spain.
Rabaan
Ali A
AA
Merino
Susana
S
Tomás
Juan M
JM
Gryllos
Ioannis
I
Shaw
Jonathan G
JG
D000333:Aeromonas / Q000235:genetics* / Q000502:physiology; D016980:Aeromonas hydrophila / Q000235:genetics* / Q000502:physiology; D001422:Bacterial Adhesion / Q000502:physiology*; D001483:Base Sequence; D018441:Biofilms / Q000254:growth & development*; D002460:Cell Line; D004269:DNA, Bacterial; D004847:Epithelial Cells / Q000382:microbiology; D005407:Flagella; D005408:Flagellin / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D005408:Flagellin