Highly sensitive chromatographic assay for dopamine determination during in vivo cerebral microdialysis in the rat.
A highly sensitive, yet simple, isocratic high-performance liquid chromatographic (HPLC) assay with electrochemical detection (ED) for the determination of extracellular dopamine (DA) in brain microdialysates is presented. The method makes possible the detection of less than 100 pM (less than 1 fmol on column) and the quantitation of 200 pM (2 fmol on column) of DA with the use of a narrow-bore rather than capillary or microbore column. Analysis is feasible within an 11-min run-time, and thus is suitable for the relatively short sampling intervals used in microdialysis experiments. In the calibration range of 0.2 to 10 nM, the method has excellent linearity and precision, with intra-day relative standard deviations (RSD) of 0.5-2.4% and between-day RSD of 2.1-4.3%.
Virag
Laszlo
L
Department of Anesthesiology, College of Physicians and Surgeons, Columbia University, 622 West 168th Street PH 5, New York, NY 10032, USA.
Whittington
Robert A
RA
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004298:Dopamine / Q000134:cerebrospinal fluid / Q000378:metabolism*; D017551:Microdialysis; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004298:Dopamine