title: Chromosomal and comparative genomic analyses of HHV-8-negative primary effusion lymphoma in five HIV-negative Japanese patients.
abstract: A rare subset of HIV lymphoma, known as primary effusion lymphoma (PEL), is a high-grade tumour carrying human herpes virus 8 (HHV-8). Very little is known about genomic aberration in PEL, and only a few HIV-negative PEL have been reported. Here we report the results of chromosomal analysis and comparative genomic hybridisation (CGH) conducted to detect regions of gain and loss, in five HIV-negative Japanese cases of HHV-8-negative PEL. All patients except one (35-year-old female) were elderly men and the morphologic examination showed large cell type. PEL expressed B-cell-associated and activation-associated antigens, and exhibited clonal immunoglobulin genes. No HHV-8 was detected in all four examined cases, but Epstein-Barr virus (EBV) was detected in one case. Genomic abnormalities and aberrations were identified in all HHV-8/HIV-negative PEL. CGH studies showed gain in 19 of 24 chromosomes. Gains of 3q13-27, 8q24, 10q21-23 and Yq were detected in two of the five cases, but other gains were noted in each case. Chromosomal analysis revealed complex abnormalities both in numbers and structures. Burkitt lymphoma-associated t(8;22) was detected in one case, but +8 chromosome and c-myc amplification were detected in the other three cases by Southern blot and/or fluorecence in situ hybridization (FISH). Abnormality of chromosome 8, which associates with c-myc, was detected in four of the five HHV-8/HIV-negative PEL. However, the other common genomic abnormalities of HHV-8/HIV-negative PEL were not detected in our study, but the complex abnormalities seemed to be true rather than the usual large B-cell lymphoma. Our results suggest that multi-step genomic abnormalities might be associated in HHV-8/HIV-negative PEL tumorigenesis.
authors: Department of Pathology, School of Medicine, Fukuoka University, Japan.
authors: Koichi
authors: K
authors: Ohshima
authors: Masako
authors: M
authors: Ishiguro
authors: Satoshi
authors: S
authors: Yamasaki
authors: Junichi
authors: J
authors: Miyagi
authors: Seichi
authors: S
authors: Okamura
authors: Yasuhiro
authors: Y
authors: Sugio
authors: Tsutomu
authors: T
authors: Muta
authors: Hidenori
authors: H
authors: Sasaki
authors: Takeshi
authors: T
authors: Tuchiya
authors: Chika
authors: C
authors: Kawasaki
authors: Masahiro
authors: M
authors: Kikuchi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000032:analysis; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis; D005260:Female; D016259:Genes, myc / Q000235:genetics; D018023:HIV Seronegativity; D004854:Herpesvirus 4, Human / Q000276:immunology; D019288:Herpesvirus 8, Human / Q000276:immunology; D006801:Humans; D007564:Japan; D016393:Lymphoma, B-Cell / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D016066:Pleural Effusion, Malignant / Q000209:etiology / Q000235:genetics* / Q000473:pathology
chemical_list: D000914:Antibodies, Viral