title: Gene-specific oxidative DNA damage in Helicobacter pylori-infected human gastric mucosa.
abstract: To study the status of oxidative DNA damage in Helicobacter pylori infection in more detail, we examined oxidative DNA damage to individual genes by determining the loss of PCR product of a targeted gene before and after gastric mucosal DNA was treated with 8-hydroxyguanine glycosylase, which cleaves DNA at the 8-hydroxyguanine residues. The results showed that, of the 5 genes tested, p53, insulin-like growth factor II receptor and transforming growth factor-beta receptor type II showed significant oxidative DNA damage in H. pylori-positive tissues and that the BAX and beta-ACTIN genes were relatively undamaged. These results suggest that in H. pylori infection, oxidative DNA damage does not occur homogeneously throughout the genomic DNA but, rather, in a gene-specific manner. We conclude that the progressive accumulation of preferential oxidative DNA damage in certain genes, such as p53, likely contributes to gastric carcinogenesis.
authors: Department of Pharmacology, College of Medicine, Seoul National University, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea.
authors: Jinhee
authors: J
authors: Choi
authors: Sun-Hee
authors: SH
authors: Yoon
authors: Ja-Eun
authors: JE
authors: Kim
authors: Kwang-Ho
authors: KH
authors: Rhee
authors: Hee-Sang
authors: HS
authors: Youn
authors: Myung-Hee
authors: MH
authors: Chung
mesh_terms: D000328:Adult; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D043382:DNA-Formamidopyrimidine Glycosylase; D004305:Dose-Response Relationship, Drug; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology*; D016158:Genes, p53 / Q000235:genetics; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D008297:Male; D009699:N-Glycosyl Hydrolases / Q000235:genetics* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases; D017527:Receptor, IGF Type 2 / Q000235:genetics; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics
chemical_list: D017527:Receptor, IGF Type 2; D018125:Receptors, Transforming Growth Factor beta; D004247:DNA; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase; D010100:Oxygen