Capillary gas chromatographic determination of 1,4-butanediol and gamma-hydroxybutyrate in human plasma and urine.
This article describes two methods for the determination of 1,4-butanediol and gamma-hydroxybutyrate in human plasma and urine using capillary gas chromatography. For 1,4-butanediol, plasma or urine samples (500 microl) were extracted by protein precipitation whereas for gamma-hydroxybutyrate, plasma or urine samples (500 microl) were extracted and derivatised with BF3-butanol. The compounds were separated on a Supelcowax-10 column and detection was achieved using a flame ionization detector. The methods are linear over the specific ranges investigated, accurate (with a percentage of the nominal concentration <109.8%) and showed intra-day and inter-day precision within the ranges of 5.0-12.0 and 7.0-10.1%, respectively. No interferences were observed in plasma and urine from hospitalized patients.
Blanchet
Benoît
B
Laboratory of Pharmacology and Toxicology, CHU Henri Mondor, Creteil, France.
Morand
Karine
K
Hulin
Anne
A
Astier
Alain
A
D002072:Butylene Glycols / Q000032:analysis* / Q000097:blood / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012978:Sodium Oxybate / Q000032:analysis* / Q000097:blood / Q000652:urine
D002072:Butylene Glycols; D012978:Sodium Oxybate; C039681:1,4-butanediol