Cloning, expression and immunization of the new antigen gene Sj-Ts4 of Schistosoma japonicum.
In order to explore the molecular mechanism of high immune protection against schistosomes infection in animals infected with Trichinella spiralis, and to provide several cross-protective antigen genes for developing anti-schistosomiasis vaccine, a Schistosoma japonicum adult worm cDNA library was immunoscreened using sera taken from mice infected with Trichinella spiralis. Nine positive clones were obtained after 3 rounds of immunoscreening. Among them, Sj-Ts4 represents a novel gene of S. japonicum, which coding for a novel protein of 210 amino acids. The protein has a deduced molecular mass of 23 kD and isoelectric point of 7.72. Sj-Ts4 was expressed as a glutathione-S-transferase (GST) fusion protein by cloning into the prokaryotic expression vector pGEX-5X-3. The recombinant Sj-Ts4 protein (rSj-Ts4) was purified and could be recognized by sera of mice infected with S.japonicum. Vaccination of several groups of mice with rSj-Ts4 or rSj-Ts4 incorporated into Freund's complete adjuvant induced high titers of specific IgG antibodies. Two vaccination groups all obtained significant reduction in worm burden (31.36%, 36.80%, P<0.01), compared with the control groups.
Zhou
Dong-Ming
DM
Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Yi
Xin-Yuan
XY
Zeng
Xian-Fang
XF
Wang
Min
M
Cai
Cai
C
Wang
Qing-Lin
QL
Zhang
Shun-Ke
SK
McReynolds
Larry
L
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015801:Helminth Proteins; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology / Q000517:prevention & control; D017422:Sequence Analysis, DNA; D013997:Time Factors
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D015801:Helminth Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C475098:Ts4 antigen, Schistosoma japonicum