Examination of the Borrelia burgdorferi transcriptome in Ixodes scapularis during feeding.
Borrelia burgdorferi gene expression within the guts of engorging Ixodes scapularis ticks was examined by use of differential immunoscreening and differential expression with a customized amplified library. Fourteen chromosomal genes involved in energy metabolism, substrate transport, and signal transduction and 10 (4 chromosomal and 6 plasmid) genes encoding putative lipoproteins and periplasmic proteins were preferentially expressed in engorging ticks. These data demonstrate a new approach to the global analysis of B. burgdorferi genes that are preferentially expressed within the vector during feeding.
Narasimhan
Sukanya
S
Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, L Diagnostics, New Haven, Connecticut 06520, USA.
Santiago
Felix
F
Koski
Raymond A
RA
Brei
Brandon
B
Anderson
John F
JF
Fish
Durland
D
Fikrig
Erol
E
D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D015139:Blotting, Southern; D025065:Borrelia burgdorferi / Q000235:genetics* / Q000378:metabolism; D005247:Feeding Behavior; D005784:Gene Amplification; D015870:Gene Expression; D015698:Genomic Library; D018884:Ixodes / Q000382:microbiology* / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger