title: Detection of highly pathogenic and low pathogenic avian influenza subtype H5 (Eurasian lineage) using NASBA.
abstract: Nucleic acid sequence-based amplification (NASBA) is a technique that allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique has been developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The NASBA technique described below is rapid and specific for the identification of influenza A subtype H5 viruses of the Eurasian lineage. More importantly, it can be used to distinguish highly pathogenic and low pathogenic strains of the H5 subtype.
authors: Hong Kong DNA Chips Ltd, 1805-6, 18/F, Lu Plaza, 2 Wing Yip Street, Hong Kong SAR, Kowloon, China.
authors: Richard A
authors: RA
authors: Collins
authors: Lung Sang
authors: LS
authors: Ko
authors: Ka Lun
authors: KL
authors: So
authors: Trevor
authors: T
authors: Ellis
authors: Lok Ting
authors: LT
authors: Lau
authors: Albert Cheung Hoi
authors: AC
authors: Yu
mesh_terms: D000818:Animals; D001717:Birds; D002642:Chick Embryo; D017931:DNA Primers; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000175:diagnosis / Q000821:virology; D008163:Luminescent Measurements; D021141:Nucleic Acid Amplification Techniques; D012367:RNA, Viral / Q000032:analysis; D013552:Swine
chemical_list: D017931:DNA Primers; D012367:RNA, Viral