Co-expression of endothelin-1 and vascular endothelial growth factor mediates increased vascular permeability in lung grafts before reperfusion.
Endothelin-1 (ET-1) protein levels increase in lung transplant recipients, although the source of ET-1 remains uncertain. Evidence is accumulating that ET-1 coregulates the expression of vascular endothelial growth/permeability factor (VEGF-A). By using real-time polymerase chain reaction and Western blotting, we describe upregulated ET-1 mRNA and VEGF-A protein levels as well as increased fluid content in donor lung-tissue biopsies before reperfusion. Hypoxia and ET-1 co-upregulate VEGF-A overexpression, therefore temporary VEGF-A antagonism during graft preservation might be of benefit in lung transplantation.
Taghavi
Shahrokh
S
Department of Cardiothoracic Surgery, University of Vienna, Vienna, Austria.
Abraham
Dietmar
D
Riml
Patricia
P
Paulus
Patrick
P
Schäfer
Romana
R
Klepetko
Walter
W
Aharinejad
Seyedhossein
S
D000328:Adult; D015153:Blotting, Western; D002199:Capillary Permeability / Q000502:physiology*; D016228:Endothelial Growth Factors / Q000032:analysis*; D019332:Endothelin-1 / Q000032:analysis*; D005260:Female; D006801:Humans; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D016040:Lung Transplantation / Q000502:physiology*; D008297:Male; D016133:Polymerase Chain Reaction; D015424:Reperfusion; D042461:Vascular Endothelial Growth Factor A
D016228:Endothelial Growth Factors; D019332:Endothelin-1; D042461:Vascular Endothelial Growth Factor A