title: MRI evaluation and functional assessment of brain injury after hypoxic ischemia in neonatal mice.
abstract: OBJECTIVE
  Severe perinatal asphyxia is an important cause of brain injury in the newborn infant. We examined early events after hypoxic ischemia (HI) in the 7-day-old mouse brain by MRI and related them to long-term functional effects and histopathology in the same animals at 4 to 5 weeks of age.
  
  
  METHODS
  HI was induced in 7-day-old CD1 mice by exposure to 8% oxygen for 30 minutes after occlusion of the left common carotid artery. The resulting unilateral focal lesion was evaluated in vivo by MRI (T2 maps and apparent diffusion coefficient maps) at 3, 6, and 24 hours and 5 days after hypoxia. Locomotion and sensorimotor function were analyzed after 3 weeks. Four weeks after HI, the mice were killed, and cresyl violet-stained brain sections were examined morphologically.
  
  
  RESULTS
  A decrease in apparent diffusion coefficient values in cortex on the affected side was found at 3 hours after HI. T2 values were significantly increased after 6 hours and remained so for 5 days. Maximal size of the lesion was attained at 3 to 6 hours after HI and declined thereafter. Animals with MRI-detected lesions had decreased forward locomotion, performed worse than controls in the beam-walking test, and showed a unilateral hypotrophy in the cresyl violet-stained brain sections 4 weeks later.
  
  
  CONCLUSIONS
  The temporal progression of the damage after HI in 7-day-old mice differs from that of the adult brain as judged by MRI. The early lesions detected by MRI were related to functional impairments for these mice in near-adult life.
authors: Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden. ulrika.aden@fyfa.ki.se
authors: Ulrika
authors: U
authors: Adén
authors: Viktoria
authors: V
authors: Dahlberg
authors: Bertil B
authors: BB
authors: Fredholm
authors: Li-Ju
authors: LJ
authors: Lai
authors: Zhengguan
authors: Z
authors: Chen
authors: Börje
authors: B
authors: Bjelke
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal; D002545:Brain Ischemia / Q000150:complications / Q000175:diagnosis* / Q000503:physiopathology; D002340:Carotid Artery Diseases / Q000150:complications; D017536:Carotid Artery, Common / Q000473:pathology / Q000502:physiology; D002540:Cerebral Cortex / Q000473:pathology*; D004058:Diffusion; D004195:Disease Models, Animal; D018450:Disease Progression; D000860:Hypoxia / Q000150:complications* / Q000503:physiopathology; D020925:Hypoxia-Ischemia, Brain / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology; D008124:Locomotion; D008279:Magnetic Resonance Imaging; D051379:Mice; D011237:Predictive Value of Tests