title: Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay--assay validation for human milk.
abstract: In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.
authors: Cosmo Oil Co Ltd, Tokyo, Japan. yukio_sugawara@cosmo-oil.co.jp
authors: Yukio
authors: Y
authors: Sugawara
authors: Koichi
authors: K
authors: Saito
authors: Masahiko
authors: M
authors: Ogawa
authors: Susumu
authors: S
authors: Kobayashi
authors: Guomin
authors: G
authors: Shan
authors: James R
authors: JR
authors: Sanborn
authors: Bruce D
authors: BD
authors: Hammock
authors: Hiroyuki
authors: H
authors: Nakazawa
authors: Yasuhiko
authors: Y
authors: Matsuki
mesh_terms: D000328:Adult; D001572:Benzofurans / Q000032:analysis / Q000633:toxicity*; D000072338:Dibenzofurans, Polychlorinated; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005544:Forecasting; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D008962:Models, Theoretical; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis / Q000633:toxicity*; D012016:Reference Values; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*
chemical_list: D001572:Benzofurans; D000072338:Dibenzofurans, Polychlorinated; D000072317:Polychlorinated Dibenzodioxins; D012989:Soil Pollutants