Donor oocyte cytoplasmic transfer did not enhance implantation of embryos of women with poor ovarian reserve.
OBJECTIVE
To determine whether donor oocyte cytoplasm transferred into the oocytes of women < or = 40 years or with diminished ovarian reserve would enhance embryo quality, implantation, or pregnancy rates.


METHODS
Study subjects included women > or = 40 years (15) or with abnormal FSH levels (3). Healthy volunteers (18) produced oocytes for cryopreservation. Donor oocytes were thawed and cytoplasm from surviving oocytes was injected with a single sperm into the cytoplasm of recipient oocytes. Outcome measures included embryo quality scores, implantation, and pregnancy rates.


RESULTS
Eighteen donors produced 213 oocytes for cryopreservation and 39/171 (22.8%) survived thawing. Eighteen recipients initiated 25 IVF cycles with embryo transfer in 20 cycles after cytoplasmic transfer (CT). Four cycles resulted in three biochemical losses and one aneuploid clinical loss. Embryo quality did not improve with CT compared to pre-CT IVF cycles in six recipients.


CONCLUSIONS
CT with cryopreserved donor oocyte cytoplasm did not enhance success in women with advanced reproductive age or low ovarian reserve.
Opsahl
M S
MS
Genetics & IVF Institute, Fairfax, Virginia 22031, USA.
Thorsell
L P
LP
Geltinger
M E
ME
Iwaszko
M A
MA
Blauer
K L
KL
Sherins
R J
RJ
D000328:Adult; D000375:Aging / Q000502:physiology*; D000782:Aneuploidy; D002470:Cell Survival; D016922:Cellular Senescence; D015925:Cryopreservation; D003593:Cytoplasm / Q000502:physiology / Q000637:transplantation*; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000503:physiopathology*; D008540:Meiosis; D008875:Middle Aged; D018587:Oocyte Donation / Q000379:methods; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure; D009866:Oogenesis; D010053:Ovary / Q000503:physiopathology*; D011247:Pregnancy; D018873:Pregnancy Rate; D020554:Sperm Injections, Intracytoplasmic
D005640:Follicle Stimulating Hormone