title: A comparative analysis of vertebrate sex determination.
abstract: Sex determination in vertebrates is controlled by a variety of mechanisms. We compared the expression of SF1, DAX1, DMRT1, SOX9 and AMH during gonadogenesis in the mouse, chicken and alligator embryo. In contrast to the expression profile of Sf1 in mouse embryos, chicken and alligator embryos show higher levels of Sf1 expression in the developing ovaries compared to testes. This may reflect the higher level of sex hormone synthesis in the ovary compared to the testis in chickens and alligators. The DAX1 gene has a similar expression profile in all three vertebrate species but appears to have different gene structure. As in mouse, DMRT1 was expressed at very high levels in the chicken and alligator male gonad. The male-specific up-regulation of SOX9 expression appears to be a common feature in all three vertebrates. In the chicken and alligator AMH is expressed prior to SOX9, suggesting that in these species SOX9 cannot initiate AMH expression as it does in mammals. SOX9 acts at multiple points in the vertebrate testis pathway but it appears that only some of these functions have been conserved through evolution.
authors: Department of Paediatrics, University of Melbourne and Murdoch Children's Research Institute, Royal Children's Hospital, Victoria, Australia.
authors: Andrew
authors: A
authors: Sinclair
authors: Craig
authors: C
authors: Smith
authors: Patrick
authors: P
authors: Western
authors: Peter
authors: P
authors: McClive
mesh_terms: D000818:Animals; D057137:DAX-1 Orphan Nuclear Receptor; D004268:DNA-Binding Proteins / Q000235:genetics; D012734:Disorders of Sex Development; D006609:High Mobility Group Proteins / Q000235:genetics; D006801:Humans; D012326:RNA Splicing; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000235:genetics; D012097:Repressor Proteins; D055758:SOX9 Transcription Factor; D019849:Sex Determination Processes; D012733:Sex Differentiation / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D057137:DAX-1 Orphan Nuclear Receptor; C110547:DMRT1 protein; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; C539965:NR0B1 protein, human; C539966:Nr0b1 protein, mouse; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins; D018168:Receptors, Retinoic Acid; D012097:Repressor Proteins; C107467:SF1 protein, human; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C491514:Sf1 protein, mouse; C527933:Sox9 protein, mouse; D014157:Transcription Factors