Characterization of the spv locus in Salmonella enterica serovar Arizona.
Salmonella enterica serovar Arizona (S. enterica subspecies IIIa) is a common Salmonella isolate from reptiles and can cause serious systemic disease in humans. The spv virulence locus, found on large plasmids in Salmonella subspecies I serovars associated with severe infections, was confirmed to be located on the chromosome of serovar Arizona. Sequence analysis revealed that the serovar Arizona spv locus contains homologues of spvRABC but lacks the spvD gene and contains a frameshift in spvA, resulting in a different C terminus. The SpvR protein functions as a transcriptional activator for the spvA promoter, and SpvB and SpvC are highly conserved. The analysis supports the proposal that the chromosomal spv sequence more closely corresponds to the ancestral locus acquired during evolution of S. enterica, with plasmid acquisition of spv genes in the subspecies I strains involving addition of spvD and polymorphisms in spvA.
Libby
Stephen J
SJ
Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695-7615, USA.
Lesnick
Marc
M
Hasegawa
Patricia
P
Kurth
Michael
M
Belcher
Christopher
C
Fierer
Joshua
J
Guiney
Donald G
DG
D036002:ADP Ribose Transferases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D017124:Conserved Sequence; D016368:Frameshift Mutation; D017353:Gene Deletion; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012104:Reptiles / Q000382:microbiology; D012480:Salmonella Infections / Q000382:microbiology*; D012476:Salmonella arizonae / Q000235:genetics* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D037521:Virulence Factors
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; C445368:SpvD protein, Salmonella enterica; D037521:Virulence Factors; C110589:tccA protein, IncP plasmid RK2; D036002:ADP Ribose Transferases; C415223:spvB protein, Salmonella enterica virulence plasmid