HPLC analysis of plasma 9-methoxycanthin-6-one from Eurycoma longifolia and its application in a bioavailability/pharmacokinetic study.
A new and simple HPLC method using fluorescence detection was developed to determine 9-methoxycanthin-6-one, an active compound of Eurycoma longifolia Jack in rat and human plasma. The method entailed direct injection of plasma sample after deproteinization using acetonitrile. The mobile phase comprised acetonitrile and distilled water (55 : 45, v/v). Analysis was run at a flow rate of 1.0 ml/min with the detector operating at an excitation wavelength of 371 nm and emission wavelength of 504 nm. The method was specific and sensitive with a detection limit of 0.6 ng/ml and a quantification limit of approximately 1.6 ng/ml. The method was applied in a pilot pharmacokinetic/bioavailability study of the compound in rats. Less than 1 % of the compound was found to be absorbed orally.
Tan
Saadiah
S
School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia.
Yuen
Kah-Hay
KH
Chan
Kit-Lam
KL
D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D002243:Carbolines; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000097:blood / Q000493:pharmacokinetics*; D010936:Plant Extracts / Q000097:blood / Q000493:pharmacokinetics*; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D029641:Simaroubaceae; D007769:Lactams
D002243:Carbolines; D009828:Oleanolic Acid; D010936:Plant Extracts; C459628:9-methoxycanthin-6-one; D007769:Lactams