title: LACK-specific CD4(+) T cells that induce gamma interferon production in patients with localized cutaneous leishmaniasis during an early stage of infection.
abstract: The profile of cytokines induced by soluble leishmania antigen (SLA) and the Leishmania homologue of the mammalian receptor for activated C kinase (LACK), a candidate vaccine against leishmaniasis, and the cellular source of the cytokines produced in response to these antigens were analyzed in patients infected with Leishmania guyanensis. Gamma interferon (IFN-gamma) and interleukin-10 (IL-10) were produced in response to LACK. Although LACK-specific CD4(+) cells producing IFN-gamma were isolated only during the early phase of infection (less than 30 days following the onset of infection), cells producing IL-10 in response to LACK were detected in all patients. CD4(+) T cells producing IFN-gamma and IL-13 were produced in response to SLA in all patients. SLA- and LACK-specific T cells are effector memory cells, as they are CD45RA(-) CCR7(-) CD4(+) T cells. CD4(+) T cells producing IFN-gamma are CD62L(-), and CD4(+) T cells producing IL-10 are CD62L(+), indicating that these cells have different tissue-homing capacities. These findings show that SLA and LACK induce both type 1 (IFN-gamma) and type 2 (IL-10 or IL-13) cell responses.
authors: Immunologie des Leishmanioses. Service d'Epidémiologie, Institut Pasteur de Cayenne, 97306 Cayenne Cedex, France.
authors: Eliane
authors: E
authors: Bourreau
authors: Ghislaine
authors: G
authors: Prévot
authors: Jacques
authors: J
authors: Gardon
authors: Roger
authors: R
authors: Pradinaud
authors: Hitoshi
authors: H
authors: Hasagewa
authors: Geneviève
authors: G
authors: Milon
authors: Pascal
authors: P
authors: Launois
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D015415:Biomarkers; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000378:metabolism; D018793:Interleukin-13 / Q000096:biosynthesis; D019041:L-Selectin; D018334:Leishmania guyanensis / Q000276:immunology*; D007897:Leishmaniasis, Mucocutaneous / Q000097:blood / Q000276:immunology* / Q000473:pathology; D017493:Leukocyte Common Antigens; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D015800:Protozoan Proteins / Q000276:immunology*; D054400:Receptors, CCR7; D019707:Receptors, Chemokine; D013997:Time Factors
chemical_list: D000953:Antigens, Protozoan; D015415:Biomarkers; C515370:CCR7 protein, human; D018793:Interleukin-13; D010455:Peptides; D015800:Protozoan Proteins; D054400:Receptors, CCR7; D019707:Receptors, Chemokine; D019041:L-Selectin; D016753:Interleukin-10; C093139:LACK antigen, Leishmania; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1