Validation of a LC-fluorescence method for determination of free captopril in human plasma, using a pre-column derivatization reaction with monobromobimane.
Both derivatization of free captopril in human plasma samples using monobromobimane as fluorescent label and the corresponding HPLC-fluorescence detection (FLD) method were validated. Calibration curve for the fluorescent captopril derivative in plasma samples is linear in the ppb-ppm range with a detection limit of 4 ppb and an identification limit of 10 ppb (P%: 90; nu > or = 5). These methods were successfully applied on bioequivalence studies carried out on some marketed pharmaceutical formulations.
Tache
Florentin
F
Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, Sos. Panduri, no. 90, Bucharest 5, Romania. flory@chem.unibuc.ro
Farca
Alexandru
A
Medvedovici
Andrei
A
David
Victor
V
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000097:blood*; D001643:Bridged Bicyclo Compounds / Q000737:chemistry*; D002138:Calibration; D002216:Captopril / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes; D006801:Humans; D015203:Reproducibility of Results; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013439:Sulfhydryl Reagents; D013810:Therapeutic Equivalency
D000806:Angiotensin-Converting Enzyme Inhibitors; D001643:Bridged Bicyclo Compounds; D005456:Fluorescent Dyes; D012997:Solvents; D013439:Sulfhydryl Reagents; D002216:Captopril; C028381:monobromobimane