title: Ultrastructural immunocytochemistry of autoimmune bullous diseases.
abstract: Advanced immunopathological assays have been developed to elucidate the pathophysiology and provide more precise nosological definitions of the immunobullous diseases. Forty-seven patients suffering from autoimmune bullous diseases (intra- or subepidermal) were studied by immunoelectron microscopy (direct and indirect). Peroxidase staining was revealed by diaminobenzidine (determination of immune deposit location) in the majority of the cases of subepidermal bullous diseases, but in less than half of the cases of intraepidermal bullous diseases. Immunoelectron microscopy features contributed in verifying the diagnosis of rare entities such as cicatricial pemphigoid, paraneoplastic autoimmune bullous disease, linear IgA disease and epidermolysis bullosa acquisita.
authors: Department of Dermatology, A Sygros Hospital, School of Medicine, National University of Athens, Greece.
authors: Anthony
authors: A
authors: Karpouzis
authors: Emmanuel
authors: E
authors: Vamvassakis
authors: Nicholas
authors: N
authors: Stavrianeas
authors: Elma
authors: E
authors: Koumantaki-Mathioudaki
authors: Myrsini
authors: M
authors: Karpouzi
authors: Anthony
authors: A
authors: Vareltzides
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D012867:Skin / Q000276:immunology* / Q000648:ultrastructure; D012872:Skin Diseases, Vesiculobullous / Q000276:immunology* / Q000378:metabolism / Q000473:pathology
chemical_list: D001323:Autoantibodies