title: Vitamin B12 and folic acid in children with intestinal parasitic infection.
abstract: OBJECTIVE
  To determine prospectively plasma levels of vitamin B12 and folic acid in children with intestinal parasitic infection before and three months after antiparasitic treatment.
  
  
  METHODS
  3036 stool samples were collected from 1959 children and 939 cello-tape anal swabs were taken from 688 children for intestinal parasite investigation. Of these, 155 children were identified as having a parasitic infection; however, only 86 were followed up during this study: 26 children with Giardia lamblia infection were treated with tinidazole and metronidazole, pyrantel pamoate was used in the treatment of 40 children with Enterobius vermicularis, and 20 patients infected with Cryptosporidium parvum received only symptomatic treatment. Vitamin B12 and folic acid levels were measured by radioimmunoassay, before and three months after the completion of treatment.
  
  
  RESULTS
  Vitamin B12 serum concentrations did not show any significant differences among the three groups. There was a significant increase in vitamin B12 serum concentrations after three months of anti-parasitic treatment (630.57+/-200.97 vs. 667.97+/-181.55 pg/dL, p = 0.002, n = 86). Paired analysis in each group showed only significant increases for vitamin B12 in the Giardia lamblia group and in the Enterobius vermicularis group. No statistically significant differences were found for folic acid serum concentrations before and three months after treatment.
  
  
  CONCLUSIONS
  Patients with symptomatic infection by Giardia lamblia and Enterobius vermicularis have lower vitamin B12 levels than asymptomatic patients. This could reflect a more affected intestinal mucous. These results could present the opportunity to treat these parasitic infections and to use vitamin B12 supplementation in symptomatic children with Giardia lamblia and Enterobius vermicularis infection.
authors: Department of Paediatrics, Lozano Blesa Hospital, University of Zaragoza School of Medicine, Spain. olivares@posta.unizar.es
authors: José L
authors: JL
authors: Olivares
authors: Ramona
authors: R
authors: Fernández
authors: Jesús
authors: J
authors: Fleta
authors: María Y
authors: MY
authors: Ruiz
authors: Antonio
authors: A
authors: Clavel
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000097:blood / Q000628:therapy; D004358:Drug Therapy; D017229:Enterobiasis / Q000097:blood / Q000188:drug therapy; D005243:Feces / Q000469:parasitology; D005260:Female; D005492:Folic Acid / Q000097:blood*; D005873:Giardiasis / Q000097:blood; D006801:Humans; D007223:Infant; D007411:Intestinal Diseases, Parasitic / Q000097:blood*; D008297:Male; D013030:Spain; D014805:Vitamin B 12 / Q000097:blood*
chemical_list: D005492:Folic Acid; D014805:Vitamin B 12