PET imaging of ischemic neuronal death in the hippocampus of living monkeys.
The aim of the present study was to visualize postischemic hippocampal neuronal death in the living monkey brain, using a high-resolution positron emission tomography (PET) and novel radioligands. In preceding papers, we reported on postischemic hippocampal neuronal death in a model of Japanese monkeys (Macaca fuscata) undergoing a 20-min complete whole-brain ischemia. Using the same model here, we investigated the in vivo bindings of two radiotracers, [11C]Ro15-4513 (a type II benzodiazepine receptor ligand) and [11C](+)3-MPB (a muscarinic cholinergic receptor ligand), in the hippocampus on day 7 after ischemia, as compared to the normal hippocampus. A significant decrease in the in vivo binding of [11C]Ro154513 and [11C(+)3-MPB was observed in the postischemic monkey hippocampus on day 7 after ischemia compared to controls. Light and electron microscopic analyses of postischemic CA1 neurons showed typical features of coagulation necrosis, as associated with a marked reduction of postsynaptic densities and presynaptic vesicles. These results suggest that semiquantification of hippocampal neuronal death is possible in the living primate brain using PET, and that the same procedures can be applied for evaluating neuronal cell loss in patients with ischemic injuries and/or dementia.
Zhao
Liang
L
Department of Neurosurgery, Kanazawa University Graduate School of Medical Sciences, Japan.
Yamashima
Tetsumori
T
Wang
Xiang-Di
XD
Tonchev
Anton B
AB
Yamashita
Junkoh
J
Kakiuchi
Takeharu
T
Nishiyama
Shingo
S
Kuhara
Shigehide
S
Takahashi
Kazuhiro
K
Tsukada
Hideo
H
D000345:Affinity Labels; D000818:Animals; D001386:Azides; D001569:Benzodiazepines; D002545:Brain Ischemia / Q000000981:diagnostic imaging* / Q000473:pathology; D002250:Carbon Radioisotopes; D016923:Cell Death; D003704:Dementia / Q000000981:diagnostic imaging / Q000473:pathology; D004195:Disease Models, Animal; D006624:Hippocampus / Q000000981:diagnostic imaging* / Q000473:pathology; D008239:Lysine / Q000031:analogs & derivatives; D008251:Macaca; D008301:Maleimides; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D011963:Receptors, GABA-A / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000378:metabolism; D014055:Tomography, Emission-Computed
D000345:Affinity Labels; D001386:Azides; D002250:Carbon Radioisotopes; D008301:Maleimides; D011963:Receptors, GABA-A; D011976:Receptors, Muscarinic; D001569:Benzodiazepines; C042957:Ro 15-4513; C047272:3-(N-maleimidopropionyl)biocytin; D008239:Lysine