[The studies of the phylogeny of acridoidea based on mtDNA sequences].
The mtDNA Cyt b sequences (432 bp) were analyzed in 10 individuals from 8 different families of Acridoidea in China. The homologous sequences were compared, the used frequency of nucleotide was calculated and the molecular phylogenetic tree constructed by Neighbor-Joining method using T. japonica as outgroup. The confidence of nodes in the trees was evaluated by bootstrap (1000 replicates). These sequences were the middle part of Cyt b gene, and in the obtained sequences, A% + T% was about 70.4% and G% + C% only 29.6%. The sequence data revealed considerable variation in 177 nucleotide sites (about 41.0%) among the analyzed individuals from 8 different families. From every amino acid codon, A% + T% in the third site was higher (86.6%) than the other two sites and lower than other insects in the corresponding region. The NJ tree suggested that 11 individuals from the 8 families clustered in 4 groups, among which Gomphoceridae and Pamphagidae firstly clustered and then together with Arcypteridae and Acrididae to form group I; Cluster II was made of three species from Catantopidae, O. japonica, O. chinensis and O. intricata; Pyrgmorphidae and Oedipodidae formed group III and Chrotogonidae single as cluster IV, respectively. The phylogenetic relationships of the 8 families was: Chrotogonidae-->Pyrgomorphidae and Oedipodidae-->Catantopidae-->Acrididae-->Arcypteridae-->Gomphoceridae and Pamphagidae, which is little different from the morphological results.
Ren
Zhu-Mei
ZM
College of Life Science and Technology, Shanxi University, Taiyuan 030006, China. zmren@sxu.edu.cn
Ma
En-Bo
EB
Guo
Ya-Ping
YP
D000818:Animals; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000737:chemistry*; D006110:Grasshoppers / Q000145:classification*; D008969:Molecular Sequence Data; D010802:Phylogeny
D003573:Cytochrome b Group; D004272:DNA, Mitochondrial