[A detection method of recombinant DNA from genetically modified potato (NewLeaf Plus potato) and detection of NewLeaf Plus potato in snack].
A detection method using polymerase chain reaction (PCR) was developed to detect the genetically modified (GM) potato (NewLeaf Plus potato; NL-P), which has not been authorized as safe in foods in Japan. The potato sucrose synthase gene was used as an internal control. The DNA from NL-P specifically provided an amplified band using PCR with a primer pair recognizing PLRV-rep gene. In addition, to prevent false-positive results in processed potato foods infected with PLRV, we designed a primer pair recognizing sequences derived from two organisms to detect specifically NL-P in processed potato. The PCR product obtained using the designed primer pair was specific for NL-P. The DNA introduced into NL-P could be detected from potato powder samples containing 0.05% NL-P. The proposed method was applied to the detection of NL-P in 25 processed potato foods. NL-P was detected in 3 snack products.
Akiyama
Hiroshi
H
National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Sugimoto
Kazue
K
Matsumoto
Misao
M
Isuzugawa
Kazuto
K
Shibuya
Masaaki
M
Goda
Yukihiro
Y
Toyoda
Masatake
M
D018744:DNA, Plant / Q000032:analysis*; D004274:DNA, Recombinant / Q000032:analysis*; D005504:Food Analysis / Q000379:methods; D005964:Glucosyltransferases / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000235:genetics*
D018744:DNA, Plant; D004274:DNA, Recombinant; D005964:Glucosyltransferases; C022699:sucrose synthase