Vascularization of the brains of the Atlantic and Pacific hagfishes, Myxine glutinosa and Eptatretus stouti: a scanning electron microscope study of vascular corrosion casts.
The microvascularization of the brains of the hagfishes, Myxine glutinosa L. and Eptatretus stouti, were studied by scanning electron microscopy (SEM) of microvascular corrosion casts. Sections of these casts were used to determine the vascular territories of defined brain areas. Histological serial sections (10 microm) of the brains served for correlation of findings. Analysis of the microvascular casts of both species revealed that the blood supply to and from these brains arose ventrally and dorsally, respectively. Neither species possesses an arterial circle (Circulus Willisi) and both have similar microvascular patterns. The only difference between Myxine and Eptatretus was that the posterior cerebral artery in Myxine divides into mesencephalic and rhombencephalic branches, and in Eptatretus a third branch, termed telencephalic branch, arises from the posterior cerebral artery. 3D-morphometry revealed that luminal diameters of: 1) intracerebral arteries and arterioles range from 35.11 +/- 5.66 microm (mean +/- SEM) in the hypothalamus to 92.69 +/- 14.48 microm in the thalamus; 2) capillaries range from 17.8 +/- 0.44 microm in the olfactory bulb to 21.70 +/- 0.87 microm in the basal ganglia; and 3) intracerebral venules and veins range from 49.38 +/- 4.17 microm in the hypothalamus to 75.58 +/- 6.59 microm in the rhombencephalon. Interbranching distances of arteries and arterioles range from 179.19 +/- 11.32 microm in the optic tectum to 235.19 +/- 94.64 microm in the hypothalamus. Capillaries range from 91.07 +/- 6.22 microm in the hypothalamus to 116.15 +/- 9.45 microm in the thalamus, and venules and veins range from 137.30 +/- 18.11 microm in the hypothalamus to 189.83 +/- 17.47 microm in the optic tectum. Intervascular distances range from 70.58 +/- 3.58 microm in the olfactory bulb to 89.52 +/- 5.74 microm in the optic tectum. Branching angles of arteries and arterioles range from 38.39 +/- 10.9 degrees in the olfactory bulb to 100.73 +/- 9.4 degrees in the optic tectum, and the branching angles of capillaries range from 74.40 +/- 5.42 degrees in the optic tectum to 90.24 +/- 4.66 degrees in the olfactory bulb. Finally, the branching angles of the venules and veins range from 67.84 +/- 6.83 degrees in the tegmentum of the mesencephalon to 92.30 +/- 6.35 degrees in the optic tectum.
Cecon
Stephan
S
University of Salzburg, Institute of Zoology, Department of Vascular- and Performance Biology, A-5020 Salzburg, Austria.
Minnich
Bernd
B
Lametschwandtner
Alois
A
D000818:Animals; D001808:Blood Vessels / Q000648:ultrastructure*; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000648:ultrastructure; D002536:Cerebral Arteries / Q000648:ultrastructure; D002550:Cerebral Veins / Q000648:ultrastructure; D016319:Corrosion Casting; D006196:Hagfishes / Q000033:anatomy & histology*; D016591:Histocytological Preparation Techniques; D008833:Microcirculation / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods*