Insulin induces PFKFB3 gene expression in HT29 human colon adenocarcinoma cells.
Fructose 2,6-bisphosphate is present at high concentrations in many established lines of transformed cells. It plays a key role in the maintenance of a high glycolytic rate by coupling hormonal and growth factor signals with metabolic demand. The concentration of fructose 2,6-bisphosphate is controlled by the activity of the homodimeric bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2). We report here the PFKFB-3 gene expression control by insulin in the human colon adenocarcinoma HT29 cell line. The incubation of these cells with 1 microM insulin resulted in an increase in the PFK-2 mRNA level after 6 h of treatment, this effect being blocked by actinomycin D. Furthermore, insulin induced ubiquitous PFK-2 protein levels, that were evident after a lag of 3 h and could be inhibited by incubation with cycloheximide.
Riera
Lluís
L
Unitat de Bioquímica i Biologia Molecular, Departament de Ciències Fisiològiques II, Campus de Bellvitge, Universitat de Barcelona, E-08907 L'Hospitalet, Spain.
Manzano
Anna
A
Navarro-Sabaté
Aurea
A
Perales
Jose C
JC
Bartrons
Ramon
R
D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006019:Glycolysis; D019073:HT29 Cells; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D025481:Phosphofructokinase-2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D013997:Time Factors
D007328:Insulin; D011506:Proteins; D012333:RNA, Messenger; D003609:Dactinomycin; D003513:Cycloheximide; C107345:PFKFB3 protein, human; D025481:Phosphofructokinase-2