title: [Determination of isothiocyanates and related compounds in mustard extract and horseradish extract used as natural food additives].
abstract: Amounts of isothiocyanates and related compounds in a mustard extract and a horseradish extract for food additive use were determined by GC, after confirmation of the identity of GC peaks by GC/MS. Amounts of allyl isothiocyanate, which included that of allyl thiocyanate, because most of the allyl thiocyanate detected in the sample was assumed to have been formed from allyl isothiocyanate during GC analysis, were 97.6% and 85.4%, in the mustard extract and the horseradish extract, respectively. Total amounts of the identified isothiocyanates in the mustard extract and the horseradish extract were 98.5% and 95.4%, respectively. Allyl cyanide, a degradation product of allyl isothiocyanate, was found in the mustard extract and the horseradish extract at the levels of 0.57% and 1.73%, respectively. beta-Phenylethyl cyanide, a possible degradation product of beta-phenylethyl isothiocyanate, and allyl sulfides were found in the horseradish extract, at the levels of 0.13% and 0.46%, respectively. Allylamine, which is another degradation product of allyl isothiocyanate, was determined after acetylation, and was found in the mustard extract and the horseradish extract at the levels of 8 micrograms/g and 67 micrograms/g, respectively.
authors: Tokyo Metropolitan Research Laboratory of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
authors: Yoko
authors: Y
authors: Uematsu
authors: Keiko
authors: K
authors: Hirata
authors: Kumi
authors: K
authors: Suzuki
authors: Kenji
authors: K
authors: Iida
authors: Tadahiko
authors: T
authors: Ueta
authors: Kunihiro
authors: K
authors: Kamata
mesh_terms: D005503:Food Additives / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D017879:Isothiocyanates / Q000032:analysis*; D009149:Mustard Plant / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis
chemical_list: D005503:Food Additives; D017879:Isothiocyanates; D010936:Plant Extracts; C058305:phenethyl isothiocyanate; C004471:allyl isothiocyanate