Enhanced expression of neuronal nitric oxide synthase and phospholipase C-gamma1 in regenerating murine neuronal cells by pulsed electromagnetic field.
Pulsed electromagnetic field (PEMF) has been shown to improve the rate of peripheral nerve regeneration. In the present study we investigated the expression of neuronal nitric oxide synthase (nNOS) and phospholipase C-gamma1 (PLC-gamma1) in regenerating rat laryngeal nerves during the exposure to PEMF after surgical transection and reanastomosis. Axons were found to regenerate into the distal stump nearly twice faster in PEMF-exposed animals than in the control. Consistently, motor function was better recovered in PEMF-treated rats. The expression of nNOS and PLC-gamma1 was highly enhanced in the regenerated nerves.
Kim
Sung Sook
SS
Department of Pathology, Ulsan University College of Medicine, Korea.
Shin
Hye Jung
HJ
Eom
Dae Woon
DW
Huh
Joo Ryung
JR
Woo
Yeongju
Y
Kim
Hyokyung
H
Ryu
Sung Ho
SH
Suh
Pann Gill
PG
Kim
Moon Jung
MJ
Kim
Jee Yun
JY
Koo
Tae Wan
TW
Cho
Yoon Hee
YH
Chung
Sung Min
SM
D000818:Animals; D004574:Electromagnetic Fields; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology / Q000528:radiation effects; D009474:Neurons / Q000502:physiology* / Q000528:radiation effects; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D051966:Phospholipase C gamma; D051381:Rats; D017207:Rats, Sprague-Dawley; D012009:Recurrent Laryngeal Nerve / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects* / Q000601:surgery; D010738:Type C Phospholipases / Q000378:metabolism*
D007527:Isoenzymes; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; D010738:Type C Phospholipases; D051966:Phospholipase C gamma