title: Effect of (1-->3)- and (1-->4)-linkages of fully sulfated polysaccharides on their anticoagulant activity.
abstract: Chemically fully sulfated polysaccharides including xylan (-->4Xylbeta-(1-->4)Xylbeta1-->), amylose (-->4Glcalpha-(1-->4)Glcalpha1-->), cellulose (-->4Glcbeta-(1-->4)Glcbeta1-->), curdlan (-->3Glcbeta-(1-->3)Glcbeta1-->) and galactan (-->3Galbeta-(1-->3)Galbeta1-->), which have been isolated from Korean clam, were prepared, and their anticoagulant activity was investigated. The results strongly suggest that the activity might not be depending on anomeric configuration (alpha or beta) or monosaccharide species but on the glycosidic linkage, either (1-->3) or (1-->4). 1H NMR studies of these modified polysaccharides show that the neighboring sulfate groups at the C-2 and C-3 positions might have caused the conformational changes of each monosaccharide from 4C(1) to 1C(4). Furthermore, the effect of 6-sulfate residues on the anticoagulant activity was investigated using a specific desulfated reaction for the chemically fully sulfated polysaccharides. The 6-sulfate group is very important in determining anticoagulant activity of (1-->3)-linked polysaccharides, whereas the activity is not affected by presence or absence of the 6-sulfate group in (1-->4)-linked polysaccharides.
authors: Graduate School of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba-shi, Chiba 263-8522, Japan.
authors: Amornrut
authors: A
authors: Chaidedgumjorn
authors: Hidenao
authors: H
authors: Toyoda
authors: Eun Rhan
authors: ER
authors: Woo
authors: Kyung Bok
authors: KB
authors: Lee
authors: Yeong Shik
authors: YS
authors: Kim
authors: Toshihiko
authors: T
authors: Toida
authors: Toshio
authors: T
authors: Imanari
mesh_terms: D000421:Alcaligenes / Q000737:chemistry; D000688:Amylose / Q000737:chemistry; D000925:Anticoagulants / Q000737:chemistry*; D029662:Betula / Q000737:chemistry; D002236:Carbohydrate Conformation; D002482:Cellulose / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D015394:Molecular Structure; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D011134:Polysaccharides / Q000737:chemistry*; D011198:Solanum tuberosum / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013431:Sulfates / Q000737:chemistry*; D047071:beta-Glucans
chemical_list: D000925:Anticoagulants; D005936:Glucans; D011134:Polysaccharides; D013431:Sulfates; D047071:beta-Glucans; C038459:curdlan; D002482:Cellulose; D000688:Amylose