Immune responses in mice to intranasal and intracutaneous administration of a DNA vaccine encoding Helicobacter pylori-catalase.
We previously reported that the intracutaneous injection of DNA vaccines encoding Helicobacter pylori heat shock proteins elicited specific immune responses, and led to reduced infection in mice. In this study, we constructed DNA vaccine encoding H. pylori-catalase (pcDNA3.1-kat) and investigated the immune responses to intranasal and intracutaneous administration of pcDNA3.1-kat. C57/BL6 mice were immunized intracutaneously with 10 microg of pcDNA3.1-kat or intranasally with 50 microg of pcDNA3.1-kat. Catalase-specific IgG antibody was detected in the sera of intranasal and intracutaneous immunized mice. Both intranasal and intracutaneous immunized mice were significantly protected from colonization by H. pylori and had significantly reduced degrees of gastritis. These results demonstrate that DNA vaccine encoding H. pylori-catalase can induce an immune response against H. pylori, and that intranasal immunization works as well as intracutaneous immunization.
Miyashita
Masayuki
M
The Second Department of Internal Medicine, Aichi Medical University, 21 Karimata, Yazako, Nagakute-Cho, Aichi, Japan.
Joh
Takashi
T
Watanabe
Katsushi
K
Todoroki
Isami
I
Seno
Kyoji
K
Ohara
Hirotaka
H
Nomura
Tomoyuki
T
Miyata
Mitsuki
M
Kasugai
Kunio
K
Tochikubo
Kunio
K
Itoh
Makoto
M
Nitta
Masakazu
M
D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002374:Catalase / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D002461:Cell Line, Transformed; D005260:Female; D015870:Gene Expression; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D013270:Stomach / Q000276:immunology / Q000473:pathology; D019444:Vaccines, DNA / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D014765:Viral Vaccines; D002374:Catalase