title: Biological monitoring of wood dust exposure in nasal lavage by high-performance liquid chromatography.
abstract: A high-performance liquid chromatography (HPLC) method for biomonitoring of occupational wood dust exposure based on nasal lavage as a biomonitoring matrix was developed. Gallic acid (GA) was chosen as the indicator compound for oak dust exposure. From the chromatographic profile of ash dust, four peaks were chosen as indicator compounds. Phenolic indicator compounds were analysed by HPLC. Personal dust samples and corresponding nasal lavage samples were collected from 16 workers exposed to oak dust and six to ash dust. The dust concentrations in the workers' breathing zone varied between 0.7 and 13.8 mg m(-3). The indicators revealed the nature of the wood dust inhaled. For the workers who did not use respirators, the correlation between the dust and corresponding indicator compound in their nasal lavage was significant; r2 = 0.59 (n = 12) for oak dust and r2 = 0.58 (n = 6) for ash dust, respectively. Further, the correlation for oak dust workers who used respirators was r = 0.67 (n = 4). Nasal lavage sampling and HPLC analysis of polyphenol indicator compounds are promising tools for measuring wood dust exposure. Although further validation is necessary, determination of the individual dose may prove invaluable in prospective epidemiological studies.
authors: Lappeenranta Regional Institute of Occupational Health, Finland.
authors: Pirjo
authors: P
authors: Mämmelä
authors: Anneli
authors: A
authors: Tuomainen
authors: Terttu
authors: T
authors: Vartiainen
authors: Lasse
authors: L
authors: Lindroos
authors: Juhani
authors: J
authors: Kangas
authors: Heikki
authors: H
authors: Savolainen
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004391:Dust / Q000009:adverse effects / Q000032:analysis*; D006801:Humans; D017817:Nasal Lavage Fluid; D016273:Occupational Exposure; D012134:Respiratory Protective Devices; D014934:Wood
chemical_list: D004391:Dust