title: Transepidermal water loss does not correlate with skin barrier function in vitro.
abstract: The purpose of this study was to investigate the relationship between transepidermal water loss and skin permeability to tritiated water (3H2O) and the lipophilic penetrant sulfur mustard in vitro. No correlation was found between basal transepidermal water loss rates and the permeability of human epidermal membranes to 3H2O (p = 0.72) or sulfur mustard (p = 0.74). Similarly, there was no correlation between transepidermal water loss rates and the 3H2O permeability of full-thickness pig skin (p = 0.68). There was no correlation between transepidermal water loss rate and 3H2O permeability following up to 15 tape strips (p = 0.64) or up to four needle-stick punctures (p = 0.13). These data indicate that transepidermal water loss cannot be unconditionally ascribed to be a measure of skin barrier function. It is clear that further work should be conducted to interpret the significance of measuring transepidermal water loss by evaporimetry.
authors: Department of Biomedical Sciences, CBD Porton Down, Salisbury, Wiltshire, UK. rchilcott@dstl.gov.uk
authors: Robert P
authors: RP
authors: Chilcott
authors: Christopher H
authors: CH
authors: Dalton
authors: Andrew J
authors: AJ
authors: Emmanuel
authors: Ceri E
authors: CE
authors: Allen
authors: Simon T
authors: ST
authors: Bradley
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003879:Dermatologic Agents / Q000493:pharmacokinetics; D015194:Diffusion Chambers, Culture; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D009151:Mustard Gas / Q000493:pharmacokinetics; D011677:Punctures; D013462:Sulfur Radioisotopes / Q000493:pharmacokinetics; D013552:Swine; D014316:Tritium / Q000493:pharmacokinetics; D014867:Water / Q000378:metabolism*
chemical_list: D003879:Dermatologic Agents; D013462:Sulfur Radioisotopes; D014867:Water; D014316:Tritium; D009151:Mustard Gas