Immunogenic protein variations of Clostridium chauvoei cellular antigens associated with the culture growth phase.
The immunoprotective capacity of four Clostridium chauvoei strains at different growth stages is reported. In all the strains tested, the cells coming from the stationary phase were those with the highest immunoprotective capacity and, depending on the strain, this protective capacity diminished or even disappeared in other phases. Protein profiles were similar in all the strains and few proteins were differentially expressed during growth as shown by SDS-PAGE. For strain 17, a local strain, a clear relationship was observed between the diminution of immunogenicity and the total loss of protective capacity of sonicated cells at late stationary phase.
Mattar
María Aída
MA
Area Microbiología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, 5700, Argentina.
Cortiñas
Teresa Inés
TI
de Guzmán
Ana María Stefanini
AM
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D001431:Bacteriological Techniques; D015153:Blotting, Western; D003013:Clostridium / Q000254:growth & development / Q000276:immunology*; D003015:Clostridium Infections / Q000276:immunology / Q000517:prevention & control; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D013010:Sonication
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007074:Immunoglobulin G