title: Rhamnose-binding lectins from steelhead trout (Oncorhynchus mykiss) eggs recognize bacterial lipopolysaccharides and lipoteichoic acid.
abstract: The interaction between bacteria and three L-rham-nose-binding lectins, named STL1, STL2, and STL3, from steelhead trout (Oncorhynchus mykiss) eggs was investigated. Although STLs bound to most Gram-negative and Gram-positive bacteria, they agglutinated only Escherichia coli K-12 and Bacillus subtilis among the bacteria tested. The binding was inhibited by L-rhamnose. STLs bound to distinct serotypes of lipopolysaccharides (LPSs), and showed much higher binding activities to smooth-type LPSs of Escherichia coli K-12 and Shigella flexneri 1A than to their corresponding rough-type LPSs. STLs also bound to lipoteichoic acid (LTA) of Bacillus subtilis. These results indicate that STLs bound to bacteria by recognizing LPSs or LTA on the cell surfaces.
authors: Department of Biological Resource Sciences, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors: Hiroaki
authors: H
authors: Tateno
authors: Tomohisa
authors: T
authors: Ogawa
authors: Koji
authors: K
authors: Muramoto
authors: Hisao
authors: H
authors: Kamiya
authors: Mineo
authors: M
authors: Saneyoshi
mesh_terms: D000371:Agglutination / Q000187:drug effects; D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000276:immunology / Q000378:metabolism*; D010063:Ovum / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D012210:Rhamnose / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D013682:Teichoic Acids / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D037102:Lectins; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid; D012210:Rhamnose