Adsorption characteristics of human plasma fibronectin in relationship to cell adhesion.
Adsorption of human plasma fibronectin (FN) on nonsulfonated and sulfonated polymer surfaces was studied, by using a polyclonal antiserum to FN and the ELISA method. ELISA signal was recorded as a function of FN concentration in solutions. The concentration dependence of FN binding shows the saturation effect in the range 5-10 microg/mL. ELISA data are discussed in the terms of a self-assembled monolayer and different conformations of the FN molecule. The early adhesion of L1210 cells to polymer surfaces after prior adsorption of FN on these surfaces was studied under static conditions. In the case of FN adsorbed on sulfonated surfaces, the relative number of adhering cells increased with the increase of the interfacial surface tension (i.e., the cell adhesion depends on the surface density of sulfonic groups). However, in the case of FN adsorbed on nonsulfonated surfaces, the relative number of adhering cells was low and independent on the interfacial surface tension. The alpha(5)beta(1)-integrin blocking by a monoclonal antibody resulted in a strong inhibition of the cell adhesion to FN adsorbed on sulfonated polymer surfaces. This indicates that cell adhesion to FN adsorbed on these surfaces is mostly mediated by the alpha(5)beta(1)-integrin. In contrast, in the case of FN adsorbed on nonsulfonated surfaces the cell adhesion was not inhibited by the alpha(5)beta(1)-integrin blocking.
Kowalczyńska
Hanna M
HM
Department of Biophysics and Biomathematics, Medical Centre for Postgraduate Education, ul. Marymoncka 99, 01-813 Warszawa, Poland. hmkowal@cmkp.edu.pl
Nowak-Wyrzykowska
Małgorzata
M
Dobkowski
Jacek
J
Kołos
Robert
R
Kamiński
Jarosław
J
Makowska-Cynka
Alicja
A
Marciniak
Ewa
E
D000327:Adsorption; D000476:Alkanesulfonates / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000493:pharmacokinetics*; D002448:Cell Adhesion / Q000187:drug effects; D005260:Female; D005353:Fibronectins / Q000097:blood / Q000378:metabolism*; D006801:Humans; D039081:Integrin alpha5beta1 / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008811:Mice, Inbred DBA; D008954:Models, Biological; D011108:Polymers / Q000493:pharmacokinetics*; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D013499:Surface Properties; D014407:Tumor Cells, Cultured
D000476:Alkanesulfonates; D001672:Biocompatible Materials; D005353:Fibronectins; D039081:Integrin alpha5beta1; D011108:Polymers