Regulation of bacterial motility in response to low pH in Escherichia coli: the role of H-NS protein.
The effect of detrimental conditions on bacterial motility in Escherichia coli was investigated. Expression profiling of mutant E. coli strains by DNA arrays and analysis of phenotypic traits demonstrated that motility and low-pH resistance are coordinately regulated. Analysis of transcriptional fusions suggests that bacterial motility in response to an acidic environment is mediated via the control by H-NS of flhDC expression. Moreover, the results suggested that the presence of an extended mRNA 5' end and DNA topology are required in this process. Finally, the presence of a similar regulatory region in several Gram-negative bacteria implies that this mechanism is largely conserved.
Soutourina
Olga A
OA
Unité de Génétique des Génomes Bactériens1 and Génopole-Plateau Protéomique2, Institut Pasteur, Paris, France.
Krin
Evelyne
E
Unité de Génétique des Génomes Bactériens1 and Génopole-Plateau Protéomique2, Institut Pasteur, Paris, France.
Laurent-Winter
Christine
C
Unité de Génétique des Génomes Bactériens1 and Génopole-Plateau Protéomique2, Institut Pasteur, Paris, France.
Hommais
Florence
F
Unité de Génétique des Génomes Bactériens1 and Génopole-Plateau Protéomique2, Institut Pasteur, Paris, France.
Danchin
Antoine
A
Unité de Génétique des Génomes Bactériens1 and Génopole-Plateau Protéomique2, Institut Pasteur, Paris, France.
Bertin
Philippe N
PN
Unité de Génétique des Génomes Bactériens1 and Génopole-Plateau Protéomique2, Institut Pasteur, Paris, France.
D001426:Bacterial Proteins; D004278:DNA, Superhelical / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005408:Flagellin / Q000096:biosynthesis* / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013489:Suppression, Genetic / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics
D001426:Bacterial Proteins; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C031908:H-NS protein, bacteria; D012333:RNA, Messenger; D015534:Trans-Activators; C090552:flhC protein, E coli; C090553:flhD protein, E coli; D005408:Flagellin