Pyrenebutanoate as a dynamic protein modifier for fluorometric detection in capillary zone electrophoresis.
Pyrenebutanoate as the amphiphilic fluorescent compound is suggested as a buffer additive in capillary zone electrophoresis (CZE) for a dynamic modification of several protein samples. Using deuterium lamp UV excitation for the on-column fluorometric detection, minimum detectable amounts in the amol-range of the proteins sampled on the CZE capillary was achieved.
Horká
Marie
M
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic. horka@iach.cz
Slais
Karel
K
D019075:Electrophoresis, Capillary / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D011506:Proteins; D011721:Pyrenes; C002218:1-pyrenebutyrate