Cross-linked polyhemoglobin-superoxide dismutase-catalase supplies oxygen without causing blood-brain barrier disruption or brain edema in a rat model of transient global brain ischemia-reperfusion.
In strokes, myocardial infarctions, severe sustained hemorrhagic shock, and donor organs, inadequate blood supply results in lack of oxygen to the tissue (ischemia). If ischemia is sustained, reperfusion with the needed oxygen can result in tissue injury (ischemia-reperfusion injury) due to formation of reactive oxygen species. We are studying an oxygen-carrying solution with anitoxidant activity formed by cross-linking hemoglobin, superoxide dismutase, and catalase to form PolyHb-SOD-CAT. The present report studies its effect on the blood-brain barrier and cerebral edema when used in a transient global brain ischemia-reperfusion rat model. We compare this solution to sham-control, oxygenated saline, stroma-free hemoglobin (SF-Hb), polymerized hemoglobin (PolyHb), and a mixture of SF-Hb, SOD, and CAT in free solution. The results show that the cross-linked PolyHb-SOD-CAT solution, unlike the other solutions, can supply oxygen to ischemic tissues without causing reperfusion injury in the transient global brain ischemia-reperfusion model.
Powanda
D Douglas
DD
Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
Chang
Thomas M S
TM
D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry / Q000494:pharmacology*; D001812:Blood-Brain Barrier / Q000502:physiology*; D001929:Brain Edema / Q000517:prevention & control; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism; D002374:Catalase / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004396:Coloring Agents / Q000493:pharmacokinetics; D005070:Evans Blue / Q000493:pharmacokinetics; D006454:Hemoglobins / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D008970:Molecular Weight; D010100:Oxygen / Q000378:metabolism; D010108:Oxyhemoglobins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D014867:Water / Q000378:metabolism
D001802:Blood Substitutes; D004396:Coloring Agents; D006454:Hemoglobins; D010108:Oxyhemoglobins; D014867:Water; D005070:Evans Blue; D002374:Catalase; D013482:Superoxide Dismutase; C086134:polyhemoglobin-superoxide dismutase-catalase; D010100:Oxygen