title: Cooperative action of alpha-glucanotransferase and maltogenic amylase for an improved process of isomaltooligosaccharide (IMO) production.
abstract: Maltogenic amylase and alpha-glucanotransferase (alpha-GTase) were employed in an effort to develop an efficient process for the production of isomaltooligosaccharides (IMOs). Bacillus stearothermophilus maltogenic amylase (BSMA) and alpha-GTase from Thermotoga maritima were overexpressed in Escherichia coli using overexpression vectors. An IMO mixture containing 58% of various IMOs was produced from liquefied corn syrup by the hydrolyzing and transglycosylation activities of BSMA alone. When BSMA and alpha-GTase were reacted simultaneously, the IMO content increased to 68% and contained relatively larger IMOs compared with the products obtained by the reaction without alpha-GTase. Time course analysis of the IMO production suggested that BSMA hydrolyzed maltopentaose and maltohexaose most favorably into maltose and maltotriose and transferred the resulting molecules simultaneously to acceptor molecules to form IMOs. alpha-GTase transferred donor sugar molecules to the hydrolysis products such as maltose and maltotriose to form maltopentaose, which was then rehydrolyzed by BSMA as a favorable substrate.
authors: Samyang Genex Biotech Research Institute, 63-2 Hwaam-Dong, Yusung-Gu, Taejeon 305-348, Korea.
authors: Hyun-Soo
authors: HS
authors: Lee
authors: Joong-Hyuck
authors: JH
authors: Auh
authors: Hyun-Geun
authors: HG
authors: Yoon
authors: Myo-Jeong
authors: MJ
authors: Kim
authors: Jin-Hee
authors: JH
authors: Park
authors: Seung-Suh
authors: SS
authors: Hong
authors: Min-Hyung
authors: MH
authors: Kang
authors: Tae-Jip
authors: TJ
authors: Kim
authors: Tae-Wha
authors: TW
authors: Moon
authors: Jung-Wan
authors: JW
authors: Kim
authors: Kwan-Hwa
authors: KH
authors: Park
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006004:Glycogen Debranching Enzyme System / Q000235:genetics / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D007534:Isomaltose / Q000096:biosynthesis; D009844:Oligosaccharides / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D014312:Trisaccharides / Q000096:biosynthesis
chemical_list: D006004:Glycogen Debranching Enzyme System; D009844:Oligosaccharides; D011994:Recombinant Proteins; D014312:Trisaccharides; C045441:isomaltotriose; C047512:isomaltotetraose; C047511:isomaltopentaose; C024046:isomaltohexose; D007534:Isomaltose; C020569:4 alpha-glucanotransferase; D006026:Glycoside Hydrolases; C120909:glucan 1,4-alpha-maltohydrolase