How does infection with parthenogenesis-inducing Wolbachia reduce the fitness of Trichogramma?
We analyzed the survival rate of the immature stages of Trichogramma species and lines that differed in their mode of reproduction. Specifically, we compared the mortality of arrhenotokous (W(-)), irrevertable thelytokous (W(-)), and Wolbachia-associated thelytokous (W(+)) forms. The embryonic mortality of the W(+) strains was significantly higher than that of the W(-) lines. The embryonic mortality was negligible for the arrhenotokous Trichogramma evanescens and the thelytokous Trichogramma cacoeciae, which is not infected with Wolbachia. Only 30% of the eggs of the Wolbachia-infected strains developed to adults, while the emergence rate of the Wolbachia-free strains was more than 78%, irrespective of the origin of the strains. More than 78% of the overall mortality in W(+) strains happened during the early stages of development. About 35% of embryos of W(+) strains remain in the mitotic stage even 48 h after oviposition. Most embryos of W(-) strains had already developed to cellular blastoderm after 6 h, regardless of strains. The mortality of immature stages in W(+) strains was mainly caused by the failure of the mitotic divisions.
Tagami
Y
Y
Hiroshima University, Higashi-Hiroshima 739-8528, Japan. tagamiy@affrc.go.jp
Miura
K
K
Stouthamer
R
R
D000818:Animals; D005260:Female; D014863:Wasps / Q000196:embryology / Q000382:microbiology*; D020577:Wolbachia / Q000502:physiology*