Micro-capillary liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry--a powerful tool for peptide and protein identification.
In order to be able to study complex biological samples, a micro-capillary liquid chromatography system was coupled to a 9.4 T Fourier transform ion cyclotron resonance mass spectrometer. The setup was tested on a tryptic digest of bovine serum albumin, which resulted in high sequence coverage (> 92%) of the protein.
Ramström
M
M
Department of Analytical Chemistry, Uppsala University, Sweden.
Palmblad
M
M
Amirkhani
A
A
Tsybin
Y O
YO
Markides
K E
KE
Håkansson
P
P
Bergquist
J
J
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D012709:Serum Albumin / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D010455:Peptides; D011506:Proteins; D012709:Serum Albumin