title: Induction of hepatocyte growth factor in stromal cells by tumor-derived basic fibroblast growth factor enhances growth and invasion of endometrial cancer.
abstract: Tumor progression is often regulated through interactions between carcinoma cells and host stromal cells. In this study of endometrial cancer, we investigated one mechanism potentially involved in hepatocyte growth factor (HGF)-mediated cancer-stromal interactions. Endometrial cancer cells (HEC-1 and ISHIKAWA) expressed the c-met receptor, but HGF did not. HGF, however, did stimulate the proliferation and invasion of these cells. The HGF gene was expressed in stromal cells, which had been separated from primary cultures of endometrial cancers, 6.4 times more than in isolated normal endometrial stromal cells. Immunohistochemical staining revealed immunoreactive HGF in cancer stromal cells, the staining intensity being more pronounced in cancer tissue than in normal endometrium. The conditioned medium from normal epithelial cells and cancer cell lines induced HGF production in normal stromal cells. We identified basic fibroblast growth factor as an HGF inducer derived from endometrial cancer cell lines. Basic fibroblast growth factor derived from tumor cells may induce HGF in endometrial stromal cells, whereas stromal cell-derived HGF leads to the invasive growth of carcinoma cells. These interactions, mediated by HGF and HGF inducers, may play a significant role in the progression of endometrial cancer.
authors: Department of Obstetrics and Gynecology, Tottori University School of Medicine, Yonago 683-8504, Japan. souichi@grape.med.tottori-u.ac.jp
authors: Souichi
authors: S
authors: Yoshida
authors: Tasuku
authors: T
authors: Harada
authors: Tomio
authors: T
authors: Iwabe
authors: Fuminori
authors: F
authors: Taniguchi
authors: Akiko
authors: A
authors: Fujii
authors: Yasuko
authors: Y
authors: Sakamoto
authors: Nobuhiro
authors: N
authors: Yamauchi
authors: Goshi
authors: G
authors: Shiota
authors: Naoki
authors: N
authors: Terakawa
mesh_terms: D002455:Cell Division / Q000187:drug effects; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology*; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000302:isolation & purification / Q000494:pharmacology*; D017228:Hepatocyte Growth Factor / Q000502:physiology*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D012016:Reference Values; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D016222:Fibroblast Growth Factor 2; D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met