The third myrosinase gene TGG3 in Arabidopsis thaliana is a pseudogene specifically expressed in stamen and petal.
Genomic clones and full-length cDNA for the myrosinase gene TGG3 from Arabidopsis thaliana ecotype Columbia were sequenced. The TGG3 gene was similar with the earlier described myrosinase genes and shared the conserved intron/exon splice sites but had an insertion of one nucleotide in exon 5, a deletion of two nucleotides in exon 6 and a deletion of approximately 210 nucleotides in exon 12. These mutations shifted the open reading frame in exon 5 and resulted in a truncated protein. Analysis of the TGG3 DNA sequence from five other Arabidopsis ecotypes showed polymorphisms, but in no case did a functional TGG3 gene appear to be present. Although TGG3 apparently is a pseudogene, it was expressed specifically in stamen and petal according to RT-PCR analysis, while TGG1 and TGG2 transcripts were present in most of the tested tissues. Western blot analysis showed only one myrosinase band of 68 kDa corresponding to TGG1 and TGG2 in flower samples, while no band corresponding to TGG3 was encountered. Apparently only two functional myrosinases are present in this gene family in Arabidopsis.
Zhang
Jiaming
J
Department of Plant Biology, Genetics Centre, Swedish University of Agricultural Sciences, Box 7080, S-750 07 Uppsala, Sweden Department of Pharmacology, College of Medicine, 835 S. Wolcott Avenue, University of Illinois at Chicago, Chicago, IL 60612, USA Department of Medical Biochemistry and Microbiology, Biomedical Centre, Box 582, Uppsala University, S-751 23 Uppsala, Sweden 1Present address: National Key Biotechnology Laboratory, Chinese Academy of Tropical Agricultural Sciences, Chengxi, Haikou, 571101, China.
Pontoppidan
Bo
B
Xue
Jiaping
J
Rask
Lars
L
Meijer
Johan
J