A new method of colorimetric assay of beta-lactamase suitable for estimation of beta-lactamase inhibition in crude microbial culture filtrates.
A simple procedure is described for the rapid assay of beta-lactamases suitable for use in the quantitation of beta-lactamase inhibition in crude culture filtrates of soil microbes. The proposed method is based on measuring the blue reaction product (lambda max 750 nm) formed during reduction of phosphomolybdic acid with reducing products generated from beta-lactam hydrolysis and is not influenced significantly by the metabolites of microbial culture filtrates which often interfere with conventional beta-lactamase assay techniques.
Basu
M
M
Microbiology Laboratory, Department of Biochemistry, University College of Science, Calcutta 700 019, India.
Sanyal
A K
AK
Banerjee
A B
AB
D039903:Actinobacteria / Q000201:enzymology* / Q000254:growth & development; D003124:Colorimetry; D003470:Culture Media; D004791:Enzyme Inhibitors / Q000378:metabolism; D005658:Fungi / Q000201:enzymology* / Q000254:growth & development; D006868:Hydrolysis; D008826:Microbial Sensitivity Tests; D008982:Molybdenum / Q000378:metabolism*; D010084:Oxidation-Reduction; D010756:Phosphoric Acids / Q000378:metabolism*; D012988:Soil Microbiology; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000378:metabolism; D047090:beta-Lactams / Q000378:metabolism
D003470:Culture Media; D004791:Enzyme Inhibitors; D010756:Phosphoric Acids; D065093:beta-Lactamase Inhibitors; D047090:beta-Lactams; D008982:Molybdenum; D001618:beta-Lactamases; C003125:phosphomolybdic acid