Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome.
Presence of porcine circovirus type 1 (PCV1) and PCV2 was studied in sera and superficial inguinal lymph nodes from postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs by using in situ hybridization and PCR. PCV1 and PCV2 were found in less than 3% and more than 50% of the samples, respectively. The most sensitive technique and site was PCR in superficial inguinal lymph nodes, but in situ hybridization correlated better with presence of characteristic lesions.
Calsamiglia
Maria
M
Centre de Recerca en Sanitat Animal (CReSA), Campus Universitari Bellaterra, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Maria.Calsamiglia@uab.es
Segalés
Joaquim
J
Quintana
Josefina
J
Rosell
Carles
C
Domingo
Mariano
M
D000818:Animals; D018173:Circoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D017925:Circovirus / Q000145:classification / Q000302:isolation & purification*; D017403:In Situ Hybridization; D008198:Lymph Nodes / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000821:virology*; D019282:Wasting Syndrome / Q000662:veterinary* / Q000821:virology