A heminested polymerase chain reaction for the detection of Brazilian rabies isolates from vampire bats and herbivores.
A heminested-PCR (hn-PCR) using primers to the nucleoprotein-coding gene in a nested set was evaluated in the detection of Brazilian strains of rabies virus (RV). A representative number of RV nucleoprotein sequences belonging to genotype 1 were aligned. Based on such alignment, primers were directed to highly conserved regions. All 42 clinical samples positive by both fluorescent antibody and mouse inoculation tests were also positive by the hn-PCR. Brain tissue that had been left to decompose, obtained from an experimentally inoculated mouse was tested by hn-PCR and yielded positive results. In conclusion, primers designed here were capable of amplifying Brazilian RV isolates obtained from a rural epidemiological cycle.
Soares
R M
RM
Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.
Bernardi
F
F
Sakamoto
S M
SM
Heinemann
M B
MB
Cortez
A
A
Alves
L M
LM
Meyer
A D
AD
Ito
F H
FH
Richtzenhain
L J
LJ
D000818:Animals; D001921:Brain / Q000276:immunology; D001938:Brazil; D002685:Chiroptera / Q000821:virology*; D017931:DNA Primers; D005455:Fluorescent Antibody Technique / Q000379:methods; D005838:Genotype; D008322:Mammals / Q000821:virology*; D051379:Mice; D009698:Nucleoproteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011818:Rabies / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D011820:Rabies virus / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D009698:Nucleoproteins