title: Ultrastructure of the early human feto-maternal interface co-cultured in vitro.
abstract: BACKGROUND
  The study was designed to investigate the ultrastructural features of the early human feto-maternal interface when generated by in-vitro co-culture, and compare these with findings reported previously from human pregnancies.
  
  
  METHODS
  Placental villi and decidua parietalis tissues from 8-12 week pregnancies were co-cultured in vitro over a 4-day period. The co-incubations were ended at 24 h intervals and processed for electron microscopical studies, and for immunocytochemistry using anti-cytokeratin antibody (CAM 5.2) for trophoblast.
  
  
  RESULTS
  Loss of the syncytium at points of contact with the decidual stroma, cytotrophoblast column formation, differentiation and invasion of extravillous trophoblast (EVT) cells into the decidual stroma over the 4-day period of co-culture were observed. Cellular components, such as actin filaments, microtubules, glycogen granules and lamellipodic processes found in EVT cells were consistent with active cellular locomotion.
  
  
  CONCLUSIONS
  These ultrastructural studies emphasize the usefulness of this model in investigating the formation of the feto-maternal interface of human pregnancy. The recruitment of cytotrophoblast to the syncytium by a process involving fusion of the intervening plasma membranes, and the migration of EVT cells causing little or no damage to the surrounding decidual cells, resemble in-vivo data.
authors: Institute of Reproductive and Developmental Biology, Wolfson & Weston Research Centre for Family Health, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK.
authors: M O
authors: MO
authors: Babawale
authors: M A
authors: MA
authors: Mobberley
authors: T A
authors: TA
authors: Ryder
authors: M G
authors: MG
authors: Elder
authors: M H F
authors: MH
authors: Sullivan
mesh_terms: D002454:Cell Differentiation; D002465:Cell Movement / Q000502:physiology; D002824:Chorionic Villi / Q000648:ultrastructure*; D018920:Coculture Techniques; D003656:Decidua / Q000166:cytology / Q000648:ultrastructure*; D005260:Female; D015726:Giant Cells / Q000166:cytology / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D011247:Pregnancy; D014327:Trophoblasts / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure