title: High-level expression and characterization of fully active recombinant conger eel galectins in Eschericia coli.
abstract: An expression system for recombinant conger eel galectins, congerins I and II, were constructed using the pTV 118N plasmid vector and Escherichia coli. Recombinant congerins I and II could be obtained in the soluble active form with high quantitative yield. Mutation of codons for Val and Leu located in the N-terminal region of Con I increased the expression efficiency. Purification of recombinant proteins were done by only two chromatographical steps from E. coli extract. The purified recombinant congerins were found to be almost the same as the native ones except for the acetyl group at the N-terminus; that is, they showed the same structures and carbohydrate binding activities, suggesting that N-terminal acetyl groups of congerins were not significant for activity.
authors: Department of Molecular Life Science, Graduate School of Life Sciences, Tohoku University, Sendai, Japan. ogawa@biochem.tohoku.ac.jp
authors: Tomohisa
authors: T
authors: Ogawa
authors: Chihiro
authors: C
authors: Ishii
authors: Yuji
authors: Y
authors: Suda
authors: Hisao
authors: H
authors: Kamiya
authors: Koji
authors: K
authors: Muramoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D017931:DNA Primers; D004524:Eels; D037161:Galectins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D017931:DNA Primers; D037161:Galectins; D011994:Recombinant Proteins; D004247:DNA