title: A Ca(2+)-inhibited non-selective cation conductance contributes to pacemaker currents in mouse interstitial cell of Cajal.
abstract: Interstitial cells of Cajal (ICC) provide pacemaker activity in some smooth muscles. The nature of the pacemaker conductance is unclear, but studies suggest that pacemaker activity is due to a voltage-independent, Ca(2+)-regulated, non-selective cation conductance. We investigated Ca(2+)-regulated conductances in murine intestinal ICC and found that reducing cytoplasmic Ca(2+) activates whole-cell inward currents and single-channel currents. Both the whole-cell currents and single-channel currents reversed at 0 mV when the equilibrium potentials of all ions present were far from 0 mV. Recordings from on-cell patches revealed oscillations in unitary currents at the frequency of pacemaker currents in ICC. Voltage-clamping cells to -60 mV did not change the oscillatory activity of channels in on-cell patches. Depolarizing cells with high external K(+) caused loss of resolvable single-channel currents, but the oscillatory single-channel currents were restored when the patches were stepped to negative potentials. Unitary currents were also resolved in excised patches. The single-channel conductance was 13 pS, and currents reversed at 0 mV. The channels responsible were strongly activated by 10(-7) M Ca(2+), and 10(-6) M Ca(2+) reduced activity. The 13 pS channels were strongly activated by the calmodulin inhibitors calmidazolium and W-7 in on-cell and excised patches. Calmidazolium and W-7 also activated a persistent inward current under whole-cell conditions. Murine ICC express Ca(2+)-inhibited, non-selective cation channels that are periodically activated at the same frequency as pacemaker currents. This conductance may contribute to the pacemaker current and generation of electrical slow waves in GI muscles.
authors: Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA.
authors: Sang Don
authors: SD
authors: Koh
authors: Jae Yeoul
authors: JY
authors: Jun
authors: Tae Wan
authors: TW
authors: Kim
authors: Kenton M
authors: KM
authors: Sanders
mesh_terms: D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002147:Calmodulin / Q000494:pharmacology; D003106:Colon / Q000502:physiology; D003238:Connective Tissue / Q000502:physiology*; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D007093:Imidazoles / Q000494:pharmacology; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009130:Muscle, Smooth / Q000502:physiology*; D009544:Niflumic Acid / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D013449:Sulfonamides
chemical_list: D000700:Analgesics; D002147:Calmodulin; D004791:Enzyme Inhibitors; D007093:Imidazoles; D007473:Ion Channels; D013449:Sulfonamides; C031938:calmidazolium; D009544:Niflumic Acid; D004533:Egtazic Acid; C017967:W 7; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium