Bursa lymphocytes and IgM-containing cells in chicken embryos bursectomized at 52-64 hours of incubation.
A technique of surgical removal of the bursal primordium ("bursectomy") of chicken embryos at stage 17, approximately 52-64 hours and 29-32 somites, is described. The survival rate of bursectomized (Bx) embryos approached a level of 50% on the 21st day. About 20% of correctly Bx embryos exhibited malformations of the anal sphincter and the large intestine. Using a rabbit anti-bursacyte serum, which did not react with thymocytes, the specific bursa-derived cell (Bu) marker was detected on the surface of bursa, spleen, bone marrow and thymus lymphocytes. Early embryonic bursectomy caused a moderate depletion of Bu marker-bearing and IgM-containing cells. It has been postulated that embryonic Bu cells can be recruited from sites other than the bursa and in the absence of the bursa.
Janković
B D
BD
Immunology Research Center, Vojvode Stepe 458, P.O. Box 979, 11000 Belgrade, Yugoslavia.
Knezević
Z
Z
Isaković
K
K
Mitrović
K
K
Marković
B M
BM
Rajcević
M
M
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002060:Bursa of Fabricius / Q000166:cytology / Q000196:embryology* / Q000276:immunology*; D002642:Chick Embryo; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D007075:Immunoglobulin M / Q000378:metabolism*; D013997:Time Factors
D000944:Antigens, Differentiation, B-Lymphocyte; D007075:Immunoglobulin M