Heterologous amplification of microsatellite markers from colubroid snakes in European natricines (serpentes: natricinae).
Eighteen microsatellite loci developed for a range of snake species (New World natricines, elapids, crotalids) were tested against European natricines (Natrix natrix, N. maura, and N. tessellata) in cross-species amplification experiments. Five loci were polymorphic (average expected heterozygosity 0.749 for a population of N. natrix in Amsterdam, mean sample size 47.8) and three loci were monomorphic. The remainder could not be consistently scored or failed to amplify. Further tests on single individuals of a diverse set of eight species of colubroid snakes showed that 15 of the 18 loci could be cross-amplified in at least one of these species. We conclude that our results show promise for the utilization of these markers for experimental assessments of genetic variation in the phylogenetically closely related group of European natricine snakes with emphasis on N. natrix. The full suite of microsatellite markers now available for snakes may show additional potential for subsequent investigation across a broader range of colubroid snakes.
Hille
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Zoologische Staatssammlung München, Muenchhausenstrasse 21, D-81247 Munich, Germany. axel.hille@zsm.mwn.de
Janssen
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Menken
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Schlegel
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Thorpe
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D000818:Animals; D017812:Colubridae / Q000235:genetics*; D017931:DNA Primers; D005060:Europe; D005784:Gene Amplification; D005819:Genetic Markers; D018895:Microsatellite Repeats
D017931:DNA Primers; D005819:Genetic Markers