title: [Clinical and pathological characteristics and pathogenesis of autoimmune hepatitis].
abstract: BACKGROUND
  To explore the clinical and pathological characteristics and pathogenesis of autoimmunohepatitis (AIH).
  
  
  METHODS
  The serum and liver biopsy specimens and clinical data of 26 cases with patients with AIH were analyzed and scored according to the criteria of International autoimmune hepatitis (IAIHG, 1999). The changes of dendritic cells (DC) in the liver tissues were observed with a panel of DC markers (CD-80/B7-1, CD-86/B7-2, CD-1a and HLA-DR) and immunohistochemistry, and the activation of hepatic stellate cells (HSC) and the expression of TGF-alpha were also detected. Liver tissue specimens from 10 patients with chronic viral hepatitis B and C respectively and 5 normal liver specimens were chosen as controls.
  
  
  RESULTS
  Mean aggregate scores of 26 AIH cases, including 21 cases of type B (80.8%) and 5 cases of type C (19.2%), which were 18.6 +/- 1.4 and 19.1 +/- 2.1 respectively. There were significant differences between the type B and type C in the average age levels of serum ALT and AST, and alpha-Glo (P <0.001 or P< 0.01 or P <0.05). Histological features of all the AIH liver tissues showed the lesions of chronic active hepatitis such as interface hepatitis/piecemeal necrosis (100%), obvious lobular inflammation (type B 95.2%, type C 100%), bridging necrosis (57.1% type B, 80.0% type C, P<0.05), rosetting of liver cells (71.4% type B, 100% type C, P<0.01), central lobular confluent necrosis (33.3% type B, 80.0% type C, P<0.001), predominant plasmacytic infiltration (type B 95.2%, type C 20.0%, P<0.001). The rates of increased and concentrated DC in the portal and lobular areas, especially in the active lesions in type B and type C AIH were 85.7% (18/21) and 5/5 respectively. It was found that DC and lymphocytes surrounded the hepatocytes which partly expressed HLA-DR antigen, while there were no or a few HLA-DR positive hepatocytes in controls. Meanwhile, the number of alpha-SMA positive HSC and the expression of TGF- were obviously increased in AIH liver tissues.
  
  
  CONCLUSIONS
  Several clinical and pathological features of AIH were identified in this study. As an antigen-presenting cell, DC might play an important role in the pathogenesis of AIH. In China, sub-type B of AIH might be more frequent than sub-type C and there were differences in clinical aspects, serology and pathology between the two types.
authors: Department of Pathology, 302nd Hosptital of PLA, Beijing 100039, China.
authors: Jingmin
authors: J
authors: Zhao
authors: Songshan
authors: S
authors: Wang
authors: Yanling
authors: Y
authors: Sun
authors: Guangde
authors: G
authors: Zhou
authors: Ping
authors: P
authors: Liu
authors: Erhong
authors: E
authors: Meng
authors: Shaojie
authors: S
authors: Xin
authors: Taihe
authors: T
authors: Zhang
authors: Fusheng
authors: F
authors: Wang
authors: Yuanli
authors: Y
authors: Mao
authors: Li
authors: L
authors: Li
authors: Yingxin
authors: Y
authors: Li
authors: Hongfei
authors: H
authors: Zhang
authors: Lingxia
authors: L
authors: Zhang
authors: Jumei
authors: J
authors: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D019693:Hepatitis, Autoimmune / Q000097:blood / Q000473:pathology*; D006801:Humans; D008099:Liver / Q000473:pathology*; D008297:Male; D008875:Middle Aged