[Production of HPV 16 major capsid protein L1 with baculovirus expression system].
BACKGROUND
To obtain human papillomavirus type 16(HPV 16) major capsid protein L1 with baculovirus expression system.


METHODS
Using pFb1 as a vector,a recombinant baculovirus expressing plasmid,which contains HPV16L1 gene sequence,was generated. The constructed virus was infected into an insect cell line Sf9.


RESULTS
After incubating at 27 degrees for 72 hours, the infected cells were collected and total cellular poteins were extracted. SDS-PAGE assay revealed a roughly 56 000 expressed protein and Western blot confirmed that the expressed protein arose from HPV16L1.


CONCLUSIONS
HPV16 later protein L1 could be efficiently expressed with baculovirus expression system,and the expressed L1 protein remains to have good immunoreactivity. This study may supply a basic work for preparing virus-like particle and prophylactic HPV subunit vaccines.
Zheng
B
B
Xian Medical University, Xian 710068,China.
Wang
J
J
Jiang
H
H
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D036022:Capsid Proteins; D002478:Cells, Cultured; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D007313:Insecta / Q000821:virology; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids / Q000235:genetics
D036022:Capsid Proteins; D009856:Oncogene Proteins, Viral; C063317:L1 protein, Human papillomavirus type 16