Altered expression of cellular genes in neutrophils of periparturient dairy cows.
The periparturient dairy cow undergoes a plethora of physiological changes, including changes in the immune system that lead to profound effects on animal health. Of the immune cells affected at parturition, the neutrophil has been of particular interest due to its primary role in innate immune defense against mastitis. Immune functions of bovine neutrophils are known to be depressed around parturition, but it has not been discerned at what level these alterations occur, including the possibility that parturition induces changes in expression of key genes. The hypothesis of the present study was that blood neutrophils respond to the physiology of parturition by altering the expression of genes needed for normal cellular functions. The main objectives of the study were to detect and characterize parturition induced changes in neutrophil gene expression, to determine if altered gene expression was significantly associated with the main steroid hormones of bovine parturition, and to obtain putative identities of differentially expressed neutrophil genes. Differential gene expression was detected and characterized through mRNA abundance changes in neutrophils, and steroid hormone concentrations by RIA assay of periparturient serum samples. Preliminary assessment of differential gene expression was done using differential display reverse transcription polymerase chain reaction (DDRT-PCR) followed by secondary screening using high throughput cDNA dot blot hybridization. Altered gene expression was confirmed using Northern blot hybridization and detailed expression patterns characterized using quantitative slot blot analysis. The identities of two fully characterized transcripts with clear parturition induced repression (P< or =0.02) in neutrophils had high DNA sequence homology with genes that encode bovine mitochondrial cytochrome b (cytb) and rig/ribosomal protein S15 (rig/RPS15). These proteins are critical for normal respiratory metabolism and translation in cells, respectively. The gene expression profiles for cytb were significantly related to serum progesterone concentration (r=0.44) and for rig/RPS15 to progesterone and estradiol concentrations (r=0.35, 0.36, respectively). Eleven additional transcripts showed evidence of parturition induced repression in neutrophils and were putatively identified as representing genes of the citric acid cycle and various DNA binding proteins. Results of this study show for the first time that genes regulating basic life functions of bovine neutrophils may be repressed by parturition, possibly due to influences of steroid hormones.
Madsen
Sally A
SA
Immunogenetics Laboratory, Department of Animal Science, 1205E Anthony Hall, Michigan State University, East Lansing 48824, USA.
Weber
Patty S D
PS
Burton
Jeanne L
JL
D000818:Animals; D015152:Blotting, Northern / Q000662:veterinary; D002417:Cattle / Q000097:blood* / Q000276:immunology; D003573:Cytochrome b Group / Q000096:biosynthesis / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D004958:Estradiol / Q000097:blood; D005260:Female; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation / Q000276:immunology*; D006854:Hydrocortisone / Q000097:blood; D007743:Labor, Obstetric / Q000097:blood* / Q000276:immunology; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics; D017422:Sequence Analysis, DNA
D003573:Cytochrome b Group; D018076:DNA, Complementary; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C036004:ribosomal protein S15; D011374:Progesterone; D004958:Estradiol; D006854:Hydrocortisone