title: Effects of histamine on phosphoinositide metabolism in chick cerebral cortex.
abstract: Effects of histamine (HA) and agonists of HA receptors on phosphoinositide metabolism in chick cerebral cortex have been studied using two approaches - measurement of inositol 1,4,5-trisphosphate (IP3) level by a specific and sensitive IP3 receptor radioassay, and analysis of [3H]inositol phosphates accumulation in cortical slices prelabeled with myo-[3H]inositol. HA concentration-dependently elevated IP3 levels in slices of chick cerebral cortex. The effect of HA was mimicked by 2-methylHA, a selective agonist of H1-HA receptors, and blocked by mepyramine, an H1 receptor antagonist. 4-MethylHA and Ralpha-methylHA, selective agonists of H2- and H3-HA receptors, respectively, did not affect IP3 level in the chick cerebrum. In cerebral cortical slices prelabeled with myo-[3H]inositol, 2-methylHA significantly stimulated [3H]inositol phosphates accumulation, whereas HA only slightly and non-significantly increased phosphoinositide metabolism. It is suggested that phospholipase C-coupled H1-HA receptors are present in the chick cerebral cortex, yet their number seems to be a small one.
authors: Department of Biogenic Amines, Polish Academy of Sciences, Lódź.
authors: J B
authors: JB
authors: Zawilsk
authors: A
authors: A
authors: Woldan-Tambor
authors: K
authors: K
authors: Kubal
authors: Z
authors: Z
authors: Nowak
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D002645:Chickens; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism*; D008297:Male; D011738:Pyrilamine / Q000494:pharmacology; D011869:Radioligand Assay
chemical_list: D006634:Histamine H1 Antagonists; D007295:Inositol Phosphates; D006632:Histamine; D015544:Inositol 1,4,5-Trisphosphate; D011738:Pyrilamine