title: Envelope-induced cell transformation by ovine betaretroviruses.
abstract: Ovine betaretroviruses include Jaagsiekte sheep retrovirus (JSRV) and enzootic nasal tumor virus (ENTV). JSRV and ENTV represent a unique class of oncogenic retroviruses that induce tumors of the respiratory tract. JSRV and ENTV are highly related but induce different diseases. Expression of the JSRV envelope (Env) induces transformation of rodent fibroblasts in vitro and phosphorylation of Akt, a central player in the phosphatidylinositol 3-kinase (PI-3K)/Akt signal transduction pathway. However, little information is available on the molecular biology of ENTV. In this study, we initially assessed whether the ENTV Env has the same properties as the homologous JSRV protein. We performed entry and interference assays using retroviral vectors pseudotyped with either the JSRV or the ENTV Env and sheep choroid plexus cells, choroid plexus cells stably expressing the JSRV Env protein, human 293T cells, mouse NIH 3T3 cells, or NIH 3T3 cells expressing human hyaluronidase 2 (HYAL2), the cellular receptor for JSRV. The results obtained indicated that ENTV and JSRV share the same receptor in sheep cells and that they can use human HYAL2 as a cellular receptor in mouse cells. The ENTV Env induces transformation of rodent fibroblasts in vitro. As with the JSRV Env, the tyrosine at position 590 is critical for ENTV Env-induced cell transformation, and Akt is phosphorylated in ENTV Env-transformed cells but not in the parental cell lines. Thus, ovine betaretroviruses share a common mechanism of cell transformation. We further investigated the relevance of Akt activation in cells transformed by ovine betaretroviruses. A PI-3K inhibitor blocked Akt phosphorylation in JSRV Env-transformed cells, suggesting a possible involvement of PI-3K in JSRV and ENTV Env-induced cell transformation. In addition, phosphorylated Akt was detected in a cell line derived from a lung tumor of a sheep with naturally occurring ovine pulmonary adenocarcinoma.
authors: Department of Medical Microbiology and Parasitology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA.
authors: Alberto
authors: A
authors: Alberti
authors: Claudio
authors: C
authors: Murgia
authors: Shan-Lu
authors: SL
authors: Liu
authors: Manuela
authors: M
authors: Mura
authors: Chris
authors: C
authors: Cousens
authors: Mike
authors: M
authors: Sharp
authors: A Dusty
authors: AD
authors: Miller
authors: Massimo
authors: M
authors: Palmarini
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D030121:Betaretrovirus / Q000235:genetics* / Q000502:physiology; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003593:Cytoplasm / Q000378:metabolism; D015686:Gene Products, env / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D022141:Jaagsiekte sheep retrovirus / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt; D051381:Rats; D011991:Receptors, Virus / Q000378:metabolism; D012756:Sheep; D014407:Tumor Cells, Cultured
chemical_list: D015686:Gene Products, env; D019869:Phosphatidylinositol 3-Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D051057:Proto-Oncogene Proteins c-akt; D011991:Receptors, Virus; C494918:AKT1 protein, human; C494920:Akt1 protein, rat