Tissue-engineered bone using mesenchymal stem cells and a biodegradable scaffold.
Bone marrow has been shown to contain a population of rare cells capable of differentiating to the cells that form various tissues. These cells, referred to as mesenchymal stem cells (MSCs), are capable of forming bone when implanted ectopically in an appropriate scaffold. The aim of this study was to investigate the potential of a new beta-tricalcium phosphate (beta-TCP) as a scaffold and to compare the osteogenic potential between beta-TCP and hydroxyapatite (HA). The beta-TCP and HA loaded with MSCs were implanted in subcutaneous sites and harvested at 1, 2, 4, and 8 weeks after implantation for biochemical and histological analysis. Biochemically, in both beta-TCP and HA composites, the alkaline phosphatase activity in the composites could be detected and was maintained at a high level for 8 weeks. In the histological analysis, active bone formation could be found in both the beta-TCP and HA composites. These findings suggest that beta-TCP could play a role as a scaffold as well as HA. The fabricated synthetic bone using biodegradable beta-TCP as a scaffold in vivo is useful for reconstructing bone, because the scaffold material is absorbed several months after implantation.
Boo
Jae Seong
JS
Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Nagoya University, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan. saiseifu@med.nagoya-u.ac.jp
Yamada
Yoichi
Y
Okazaki
Yasuhiro
Y
Hibino
Yoshitaka
Y
Okada
Kunihiko
K
Hata
Ken-Ichiro
K
Yoshikawa
Takafumi
T
Sugiura
Yasuo
Y
Ueda
Minoru
M
D020341:Absorbable Implants; D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells; D002130:Calcium Phosphates; D017886:Durapatite; D008297:Male; D008648:Mesoderm / Q000166:cytology; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis; D051381:Rats; D011916:Rats, Inbred F344; D013234:Stem Cells / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D015675:Osteocalcin; D017886:Durapatite; D000469:Alkaline Phosphatase; C018392:tricalcium phosphate