Induction of human herpesvirus 8 gene expression in a posttransplantation primary effusion lymphoma cell line.
Human herpesvirus 8 (HHV-8 or Kaposi's sarcoma herpesvirus) is a gamma herpesvirus that is most likely the etiologic agent of both Kaposi's sarcoma and primary effusion lymphoma (PEL), a rare HIV-associated lymphoma. The role of HHV-8 in post-transplant lymphoma is less well characterized. We demonstrate that HHV-8 is constitutively present in LH5-21 cells, an atypical patient derived posttransplant PEL cell line. LH5-21 cells lack detectable Epstein-Barr virus, express T cell-associated surface markers and have undergone immunoglobulin heavy chain gene rearrangement. Incubation with 12-O-tetradecanoyl-phorbol- 13-acetate or butyrate induces high levels of several HHV-8 encoded genes that are associated with lytic replication. The patient from whom this cell line was derived demonstrated a dramatic clinical response to withdrawal of immunosuppressive therapy. While HHV-8 associated PELs in the post-transplant setting are rare, this study suggests that improvement in the host immunologic function might help in the management of some PELs.
Shaw
Renee N
RN
Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA.
Waller
Edmund K
EK
Offermann
Margaret K
MK
D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D019288:Herpesvirus 8, Human / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D016867:Immunocompromised Host; D016030:Kidney Transplantation; D016393:Lymphoma, B-Cell / Q000188:drug therapy / Q000209:etiology / Q000821:virology*; D016066:Pleural Effusion, Malignant / Q000188:drug therapy / Q000209:etiology / Q000821:virology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D013755:Tetradecanoylphorbol Acetate