title: Septal grafts and evoked acetylcholine release in the rat hippocampus after 192 IgG-saporin lesions.
abstract: Adult rats received intraseptal injections of 192 IgG-saporin and intrahippocampal grafts of septal cells. Between 6 and 10 months later, we assessed baseline and electrically-evoked release of tritium in hippocampal slices preloaded with [(3)H]choline, and the uptake of [(3)H]choline, [(3)H]noradrenaline and [(3)H]serotonin by hippocampal synaptosomes. The lesions reduced the accumulation of [(3)H]choline by approximately 40%, the evoked release of [(3)H]acetylcholine by approximately 90%, and the uptake of [(3)H]choline by synaptosomes by 90% in the dorsal hippocampus, but increased the relative baseline release of [(3)H]choline by +43%, and the synaptosomal uptake of [(3)H]noradrenaline (66%) and [(3)H]serotonin (58%) in the ventral hippocampus. The increased noradrenaline uptake may account for sympathetic ingrowth. Although the grafts of fetal septal neurons produced modest cholinergic effects, these effects were positive and significant.
authors: Institut für Experimentelle und Klinische Pharmakologie und Toxikologie der Universität Freiburg, Neuropharmakologisches Labor, Hansastrasse 9A, D-79104 Freiburg, Germany.
authors: Anja
authors: A
authors: Birthelmer
authors: Esther
authors: E
authors: Dommes
authors: Hélène
authors: H
authors: Jeltsch
authors: Jean-Christophe
authors: JC
authors: Cassel
authors: Rolf
authors: R
authors: Jackisch
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000633:toxicity*; D016380:Brain Tissue Transplantation / Q000379:methods; D018678:Cholinergic Agents / Q000633:toxicity*; D004558:Electric Stimulation / Q000379:methods; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation*; D000922:Immunotoxins / Q000633:toxicity*; D007276:Injections, Intraventricular; D008297:Male; D009699:N-Glycosyl Hydrolases; D051381:Rats; D020318:Rats, Long-Evans; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D020665:Septum of Brain / Q000637:transplantation*
chemical_list: C085696:192 IgG-saporin; D000911:Antibodies, Monoclonal; D018678:Cholinergic Agents; D000922:Immunotoxins; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins; D000109:Acetylcholine