Basal levels of DNA strand breaks in human leukocytes determined by comet assay.
In order to determine background levels of DNA strand breaks, we examined 80 healthy individuals by comet assay considering age, sex, and smoking as confounding factors. Only age was found to have a significant effect on basal levels. One thousand cells of each donor were graded by eye into 5 categories according to the amount of DNA in the tail: classification group A (no damage) <5%, B (low damage) 5-20%, C (medium damage) 20-40%, D (high damage) 40-95%, and group E (total damage) >95%. The interpretation of the comet assay was modified to achieve a tail factor, which represents the DNA damage of 1000 scored cells as a single number, without the need of an image analysis software package. Hydrogen peroxide and bleomycin used for in vitro exposure of lymphocytes, produced clear dose-related responses in the comet assay. Our data encourage the application of the used classification model for a sensitive and fast quantification of DNA damage. Results in this study are in agreement with most of the earlier investigations.
Diem
Elisabeth
E
Department of Occupational Medicine, University of Vienna, Austria. Elisabeth.Diem@akh-wien.ac.at
Ivancsits
Sabine
S
Rüdiger
Hugo W
HW
D000328:Adult; D000375:Aging / Q000473:pathology; D000465:Algorithms; D001761:Bleomycin / Q000494:pharmacology; D004396:Coloring Agents; D020552:Comet Assay; D004249:DNA Damage; D003627:Data Interpretation, Statistical; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007962:Leukocytes / Q000737:chemistry* / Q000473:pathology; D008297:Male; D016877:Oxidants / Q000494:pharmacology; D012016:Reference Values; D012727:Sex Characteristics; D012907:Smoking / Q000473:pathology
D004396:Coloring Agents; D016877:Oxidants; D001761:Bleomycin; D006861:Hydrogen Peroxide