An ATP-dependent step is required for the translocation of microinjected precursor mRNA into nuclear speckles.
Nuclear speckles (speckles) represent a distinct nuclear compartment within the interchromatin space and are enriched in splicing factors. In a previous study (Melcák et al., 2001), it has been shown that the pre-spliceosomal assembly on microinjected splicing-competent precursor mRNA takes place in the speckles, and it has been suggested that the targeting of RNA into speckes consists of two interdependent steps, namely the diffusion process, followed by the energy-dependent translocation of RNA into the speckles. In the present study, we confirm the existence of these two steps and show that this latter translocation is ATP dependent.
Kopský
V
V
Department of Cell Biology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague.
Vecerová
J
J
Melcák
I
I
Pliss
A
A
Stulík
J
J
Koberna
K
K
Tomásková
L
L
Raska
I
I
D000255:Adenosine Triphosphate / Q000378:metabolism*; D022003:Cell Nucleus Structures / Q000378:metabolism*; D005260:Female; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D008845:Microinjections; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D012326:RNA Splicing; D034443:RNA Transport; D017381:Spliceosomes / Q000235:genetics / Q000378:metabolism
D005456:Fluorescent Dyes; D012322:RNA Precursors; D000255:Adenosine Triphosphate