title: Measurement of corticoids in the patients with clinical features indicative of mineralocorticoid excess.
abstract: BACKGROUND
  A method for the measurement of five important serum and urinary corticoids on the syndrome of mineralcorticoid excess is reported. The methodology was combined gas chromatography-mass spectrometry (GC-MS) with selected ion-monitoring mode.
  
  
  METHODS
  After extraction with a solid-phase cartridge using an Oasis HLB copolymer, the residues were derivatized with a mixture of N-methyl-N-trimethylsilyltrifluoroacetamide/ammonium iodide/dithioerythritol (1000:4:5, v/w/w), and analyzed.
  
  
  RESULTS
  The linearity as the regression coefficients were >0.979 over a range of 1-500 ng/ml, and limit of detection ranged from 1 to 3 ng/ml while their analytical recoveries varied in the range of 75.7-94.9%. The overall precision (% CV) of the method were 3.2-7.2% and 3.6-6.3% for serum and urine, respectively. The accuracy expresses as % bias ranged from -4.1 to 6.4%. This assay was used on two patients with hypokalemic hypertension, and may be useful in ruling out mineralcorticoid excess (AME) type 1 or 2.
  
  
  CONCLUSIONS
  The present GC-MS technique may be useful to differentiate between the syndrome of AME and other hypertensive diseases with clinical features suggestive of mineralcorticoid excess because of the assay's reliablity and precision.
authors: Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 130-650, South Korea.
authors: Man Ho
authors: MH
authors: Choi
authors: Jong Ryeal
authors: JR
authors: Hahm
authors: Byung Hwa
authors: BH
authors: Jung
authors: Bong Chul
authors: BC
authors: Chung
mesh_terms: D000305:Adrenal Cortex Hormones / Q000097:blood* / Q000378:metabolism / Q000652:urine*; D000328:Adult; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D008659:Metabolic Diseases / Q000097:blood / Q000378:metabolism / Q000652:urine; D008901:Mineralocorticoids / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000305:Adrenal Cortex Hormones; D008901:Mineralocorticoids