Peptide differential display: a novel approach for phase transition in locusts.
Today, the question of the physiological cause of phase transition, the transition from the solitary to the gregarious phase, in locusts remains unanswered. We hereby present a novel approach by which we have attempted to determine whether different phases express or release different peptides in similar physiological conditions. For this purpose, a peptidomic analysis of the corpora cardiaca and hemolymph of crowded and isolated locusts of Schistocerca gregaria was performed using high performance liquid chromatography and matrix-assisted laser desorption ionisation time of flight mass spectrometry. A comparison between the two conditions reveals differences in the number and amount of peptides present in the corpora cardiaca and the hemolymph. Further research will have to identify these phase specific differences and their role in locust phase polymorphism.
Clynen
Elke
E
Laboratory for Developmental Physiology and Molecular Biology, K.U. Leuven, Leuven, Belgium. elke.clynen@bio.kuleuven.ac.be
Stubbe
Dirk
D
De Loof
Arnold
A
Schoofs
Liliane
L
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006110:Grasshoppers; D006458:Hemolymph / Q000378:metabolism; D008297:Male; D009490:Neurosecretory Systems / Q000378:metabolism; D010455:Peptides / Q000737:chemistry*; D011110:Polymorphism, Genetic; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D010455:Peptides