Characterization and comparison of commercially available German and American cockroach allergen extracts.
BACKGROUND
In this study we examine the variability among unstandardized cockroach allergen extracts.


METHODS
We obtained 24 aqueous and glycerinated cockroach allergen extracts from nine manufacturers. We used previously characterized cockroach extracts, E2-Cg and E2-Ca, as references. The modified ninhydrin assay was used to determine protein concentration of each extract. Relative potencies of extracts were determined by competition ELISA, using a human allergic serum pool. Bla g 1 and Bla g 2 levels of glycerinated German cockroach extracts were determined by ELISA using monoclonal antibodies. Extracts were also analysed by SDS-PAGE.


RESULTS
Commercial cockroach allergen extracts had highly variable protein contents that were lower than the protein contents of the references. Electrophoretic data confirmed the presence of a variable number and intensity of protein bands in extracts among manufacturers. The relative potencies of the commercial extracts were between 10 and 782 BAU/mL for German cockroach and 10-250 BAU/mL for American cockroach. The mean Bla g 1 content of the commercial extracts was significantly lower than that of the reference (P = 0.001). The mean Bla g 2 content of the commercial extracts was higher than that of the E2-Cg reference but the Bla g 2 levels were more variable compared to Bla g 1. In glycerinated German cockroach extracts, protein concentrations, relative potencies and specific allergen levels were significantly correlated (P < 0.001).


CONCLUSIONS
Our tests indicate that commercially available cockroach allergen extracts are variable in protein content, electrophoretic banding patterns, relative potency and Bla g 2 levels. In glycerinated German cockroach extracts, protein concentrations, relative potencies and specific allergen levels were significantly correlated.
Patterson
M L
ML
Laboratory of Immunobiochemistry, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Bethesda 20852, USA. pattersonm@cber.fda.gov
Slater
J E
JE
D000485:Allergens / Q000737:chemistry*; D000569:Americas; D000818:Animals; D052179:Antigens, Plant; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry* / Q000592:standards; D004591:Electrophoresis, Polyacrylamide Gel; D005858:Germany; D019476:Insect Proteins / Q000032:analysis; D012015:Reference Standards; D012016:Reference Values; D014020:Tissue Extracts / Q000737:chemistry
D000485:Allergens; D052179:Antigens, Plant; D019476:Insect Proteins; D014020:Tissue Extracts; C105482:allergen Bla g 1; D016282:Aspartic Acid Endopeptidases; C094699:allergen Bla g 2