Pro-EMAP II is not primarily cleaved by caspase-3 and -7.
Endothelial monocyte-activating polypeptide (EMAP) II is a unique cytokine, also known as p43, the active mature form of which exhibits antiangiogenic properties in vivo and in vitro. The proteolytic enzymes associated with the cleavage and release of the active mature form, however, remain unclear. Here we show that, in contrast to prior observations, purified pro-EMAP II is not cleaved by either caspase-3 or -7 in vivo or in vitro. Thus other proteolytic processes, which allow it to induce apoptosis via caspase-3 activation in migrating and dividing endothelium, may be involved in the release of the active mature EMAP II.
Zhang
F R
FR
Departments of Pediatrics, Cardiothoracic Surgical Research, and Surgery, Childrens Hospital Research Institute, Los Angeles 90027, USA.
Schwarz
M A
MA
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3; D053179:Caspase 7; D020169:Caspases / Q000737:chemistry / Q000378:metabolism*; D018920:Coculture Techniques; D016207:Cytokines; D004789:Enzyme Activation / Q000187:drug effects; D005047:Etoposide / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D019169:Jurkat Cells; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D051379:Mice; D009363:Neoplasm Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology
D016207:Cytokines; D009363:Neoplasm Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D016601:RNA-Binding Proteins; C077219:small inducible cytokine subfamily E, member 1; D005047:Etoposide; C505407:CASP3 protein, human; C505520:CASP7 protein, human; C505409:Casp3 protein, mouse; C505524:Casp7 protein, mouse; D053148:Caspase 3; D053179:Caspase 7; D020169:Caspases