Characterisation of sucrose-negative Yersinia enterocolitica 4/O:3 isolates recovered from pig tonsils.
Sucrose-negative Yersinia enterocolitica isolates of bioserotype 4/O:3 have been recovered for the first time. They were found in 2% of the tonsils of clinically healthy fattening pigs. These sucrose-negative Y. enterocolitica isolates could not be differentiated from Y. kristensenii isolates using API 20E; thus, they were identified using PCR and sequencing. Using pulsed-field gel electrophoresis (PFGE). NotI profiles of sucrose-negative Y. enterocolitica 4/O:3 isolates showed a high similarity to sucrose-positive Y. enterocolitica 4/O:3 isolates. This study demonstrated that sucrose-negative Y. enterocolitica 4/O:3 isolates of porcine origin can harbour virulence genes; plasmid-encoded virulence markers were found in 8 out of 11 isolates and all isolates contained chromosomal-encoded virulence markers. Thus, the pathogenicity of sucrose-negative Yersinia isolates should always be assessed.
Fredriksson-Ahomaa
M
M
Department of Food and Environmental Hygiene, Helsinki University, Finland. mkufa@yahoo.com
Niskanen
T
T
Neubauer
H
H
Laukkanen
R
R
Korkeala
H
H
D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D014066:Palatine Tonsil / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012703:Serotyping / Q000379:methods / Q000662:veterinary; D013395:Sucrose / Q000378:metabolism*; D013552:Swine; D014774:Virulence; D015008:Yersinia enterocolitica / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity
D004269:DNA, Bacterial; D013395:Sucrose