title: Diversity of glutamate dehydrogenase in human brain.
abstract: Three forms of glutamate dehydrogenase (GDH, EC 1.4.1.3) are purified from human brain tissue. Two of them, named GDH I (consisting of 58+/-1-kDa subunit) and GDH II (consisting of 56+/-1 -kDa subunit), are readily solubilized and the third one, GDH III (consisting of 56+/-1-kDa subunit), is a membrane-associated (particulate bound) isoform. Kinetic constants were determined for GDH III. These GDH forms were found to differ in hydrophobicity as indicated by different affinity to Phenyl-Sepharose. All three GDH forms showed microheterogeneity on two-dimensional (2-D) gel electrophoresis. Specific polyclonal antibodies, which enable to determine the levels of immunoreactivities of all the GDH forms in human brain extracts by enzyme-chemiluminescent amplified (ECL)-Western immunoblotting, were obtained.
authors: Laboratory of Neurochemistry, Mental Health Research Center RAMS, Moscow, Russia. burbaeva@rcmh.msk.ru
authors: Gulnur Sh
authors: GSh
authors: Burbaeva
authors: Marina S
authors: MS
authors: Turishcheva
authors: Elena A
authors: EA
authors: Vorobyeva
authors: Olga K
authors: OK
authors: Savushkina
authors: Elena B
authors: EB
authors: Tereshkina
authors: Irina S
authors: IS
authors: Boksha
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000276:immunology; D002417:Cattle; D005969:Glutamate Dehydrogenase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera / Q000378:metabolism; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D013552:Swine
chemical_list: D007106:Immune Sera; D007527:Isoenzymes; D005969:Glutamate Dehydrogenase