A PCR assay for rapid detection of vancomycin-resistant enterococci.
Since the first report of a vancomycin-resistant enterococcal clinical isolate, these Gram-positive bacteria have emerged as important nosocomial pathogens. Several glycopeptide resistance phenotypes can be distinguished on the basis of the level and inducibility of resistance to vancomycin and teicoplanin. In the present study, we developed a multiplex PCR, which allows the simultaneous identification of enterococci at the genus level and detection of the most frequent glycopeptide resistance genotypes. Five primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 and tuf were used in one reaction tube with bacterial DNA extracted from three to five colonies. This PCR method is suitable for the rapid detection of vancomycin-resistant enterococci.
Pérez-Hernández
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X
Molecular Biology Laboratory, Research Unit, Nuestra Señora de Candelaria University Hospital, Santa Cruz de Tenerife, Spain.
Méndez-Alvarez
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Claverie-Martín
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D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D016983:Enterococcus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D020713:Vancomycin Resistance
D004269:DNA, Bacterial