Immunological protection induced by bacillus Calmette-Guérin treatment in a murine bladder tumor model.
BACKGROUND
It has been previously reported that MBT-2 tumor growth is completely inhibited when mice are inoculated with bacillus Calmette-Guérin (BCG). In this study it was examined whether or not vaccination with a mixture of BCG and MBT-2 cells also induces immunological protection against murine bladder tumors.


METHODS
Seven hundred thousand MBT-2 cells and 1 mg of BCG (Tokyo 172 strain) per mouse were injected subcutaneously into female C3H/HeN mice. Four and eight weeks after vaccination with this mixture, animals were reinoculated with MBT-2 cells alone or MBT-2 cells cocultured with BCG.


RESULTS
Animals vaccinated with a mixture of BCG and MBT-2 cells showed MBT-2 tumor growth but completely rejected the MBT-2 cells cocultured with BCG. MBT-2 cells cocultured with BCG developed into tumors when they were inoculated into the control animals. Splenocytes prepared from vaccinated animals showed specific cytocidal activity against MBT-2 cells precultured with BCG.


CONCLUSIONS
The results suggest that a mixture of BCG and MBT-2 cells induces antitumor immunological protection against BCG- or MBT-2-associated antigens presented on MBT-2 cells precultured with BCG.
Iwasaki
Akio
A
Department of Urology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba city, Ibaraki, Japan.
Kawai
Koji
K
Hayashi
Hitoshi
H
Ikeda
Noriko
N
Toida
Ichiro
I
Ohtani
Mikinobu
M
Akaza
Hideyuki
H
D000818:Animals; D001500:BCG Vaccine / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000473:pathology; D002295:Carcinoma, Transitional Cell / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D016233:Immunotherapy, Active; D051379:Mice; D008809:Mice, Inbred C3H; D001749:Urinary Bladder Neoplasms / Q000276:immunology* / Q000473:pathology
D001500:BCG Vaccine