title: Regulation of bacterial motility in response to low pH in Escherichia coli: the role of H-NS protein.
abstract: The effect of detrimental conditions on bacterial motility in Escherichia coli was investigated. Expression profiling of mutant E. coli strains by DNA arrays and analysis of phenotypic traits demonstrated that motility and low-pH resistance are coordinately regulated. Analysis of transcriptional fusions suggests that bacterial motility in response to an acidic environment is mediated via the control by H-NS of flhDC expression. Moreover, the results suggested that the presence of an extended mRNA 5' end and DNA topology are required in this process. Finally, the presence of a similar regulatory region in several Gram-negative bacteria implies that this mechanism is largely conserved.
authors: Unité de Génétique des Génomes Bactériens1 and Génopole-Plateau Protéomique2, Institut Pasteur, Paris, France.
authors: Olga A
authors: OA
authors: Soutourina
authors: Unité de Génétique des Génomes Bactériens1 and Génopole-Plateau Protéomique2, Institut Pasteur, Paris, France.
authors: Evelyne
authors: E
authors: Krin
authors: Unité de Génétique des Génomes Bactériens1 and Génopole-Plateau Protéomique2, Institut Pasteur, Paris, France.
authors: Christine
authors: C
authors: Laurent-Winter
authors: Unité de Génétique des Génomes Bactériens1 and Génopole-Plateau Protéomique2, Institut Pasteur, Paris, France.
authors: Florence
authors: F
authors: Hommais
authors: Unité de Génétique des Génomes Bactériens1 and Génopole-Plateau Protéomique2, Institut Pasteur, Paris, France.
authors: Antoine
authors: A
authors: Danchin
authors: Unité de Génétique des Génomes Bactériens1 and Génopole-Plateau Protéomique2, Institut Pasteur, Paris, France.
authors: Philippe N
authors: PN
authors: Bertin
mesh_terms: D001426:Bacterial Proteins; D004278:DNA, Superhelical / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005408:Flagellin / Q000096:biosynthesis* / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013489:Suppression, Genetic / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C031908:H-NS protein, bacteria; D012333:RNA, Messenger; D015534:Trans-Activators; C090552:flhC protein, E coli; C090553:flhD protein, E coli; D005408:Flagellin