Collagenase production in an antarctic strain of Arthrobotrys tortor Jarowaja.
This paper describes the results of a comparative screening between the nematophagous Antarctic fungus Arthrobotrys tortor and other species of that genus for the production of extracellular collagenases. The nematode species used in this study was Caenorhabditis elegans, feeding on Escherichia coli cultures. Determination of collagenase activity was made using insoluble collagen from bovine Achilles tendon and determining the amount of solubilized hydroxyproline produced. The results show that the total amount of collagenase produced by the Antarctic strain of A. tortor was about threefold higher than that observed for the other species. In the Antarctic strain, collagenase was shown to be a constitutive enzyme.
Tosi
Solveig
S
Università di Pavia, Italy.
Annovazzi
Laura
L
Tosi
Ilaria
I
Iadarola
Paolo
P
Caretta
Giuseppe
G
D000818:Animals; D000864:Antarctic Regions; D001673:Biodegradation, Environmental; D017173:Caenorhabditis elegans / Q000254:growth & development / Q000382:microbiology; D003094:Collagen / Q000378:metabolism; D017364:Collagenases / Q000096:biosynthesis*; D006909:Hydroxyproline / Q000032:analysis / Q000096:biosynthesis; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000472:pathogenicity; D013045:Species Specificity
D003094:Collagen; D017364:Collagenases; D006909:Hydroxyproline