Permeation of macromolecules into polyelectrolyte microcapsules.
Polyelectrolyte microcapsules (PEMCs) have been prepared by coating red blood cells with the polyelectrolytes poly(styrenesulfonate), poly(allylamine hydrochloride), and dextran sulfate applying the layer-by-layer technique with subsequent dissolution of the core. The capsule permeability for human serum albumin (HSA) was studied as a function of the ionic strength and pH by means of confocal microscopy. PEMCs produced with dextran sulfate and poly(allylamine hydrochloride) show a significant increase in permeability for HSA at salt concentrations over 1 mM. For PEMCs prepared with poly(styrenesulfonate) and poly(allylamine hydrochloride) the limiting salt concentration is 5 mM. No pH dependence for permeation was observed. A correlation between the permeation and adsorption of HSA on the PEMC walls was investigated. Finally, a mechanism for the permeability, combining electrostatic interactions, and the presence of pores in the polymer layers is presented confirmed by the considerable increase of permeation of charged molecules in the presence of salt and the permeation of neutral molecules regardless of the ionic strength.
Georgieva
R
R
Institute of Transfusion Medicine, Medical Faculty Charité, Humboldt University of Berlin, Berlin, Germany.
Moya
S
S
Hin
M
M
Mitlöhner
R
R
Donath
E
E
Kiesewetter
H
H
Möhwald
H
H
Bäumler
H
H
D001704:Biopolymers / Q000493:pharmacokinetics*; D002214:Capsules / Q000737:chemistry*; D016264:Dextran Sulfate / Q000737:chemistry; D004912:Erythrocytes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D009994:Osmolar Concentration; D010539:Permeability; D011073:Polyamines / Q000737:chemistry; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D012709:Serum Albumin / Q000493:pharmacokinetics
D001704:Biopolymers; D002214:Capsules; D016264:Dextran Sulfate; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers; D011137:Polystyrenes; D012709:Serum Albumin; C063994:polyallylamine; C003321:polystyrene sulfonic acid