title: Tenascin in the human intervertebral disc: alterations with aging and disc degeneration.
abstract: Our objective for this study was to determine the presence and distribution of tenascin in the human intervertebral disc. The tenascins are a family of extracellular matrix proteins with repeated structural domains homologous to epidermal growth factor, fibronectin type III and the fibrinogens. Little is known about the presence of this protein in the disc. Ten normal human discs donated from subjects newborn to 15 years old, 10 control discs from adult donors aged 24-41 years, and 11 surgical disc specimens from patients aged 26-76 years were examined for immunolocalization of tenascin. In young discs, tenascin was localized throughout the annulus; in the nucleus, localization was confined to pericellular matrix. In adult control and degenerating disc specimens, tenascin in the annulus was localized primarily in pericellular matrix regions encircling either single cells or clusters of disc cells; in rare instances localization was more diffuse in the intraterritorial matrix. In young, healthy disc, tenascin was abundant throughout the annulus. In contrast, degenerating discs in adults showed a localization restricted to the pericellular, and rarely, more restricted intraterritorial matrix. These observations indicate that changes in the amount and distribution of tenascin may have a role in disc aging and degeneration, possibly by modulating fibronectin-disc-cell interactions, and causing alterations in the shape of disc cells.
authors: Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, North Carolina 28232, USA. hgruber@carolinas.org
authors: H E
authors: HE
authors: Gruber
authors: J A
authors: JA
authors: Ingram
authors: E N
authors: EN
authors: Hanley
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism* / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007223:Infant; D007403:Intervertebral Disc / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D019063:Tenascin / Q000378:metabolism*
chemical_list: D019063:Tenascin