title: Incidence of Norwalk-like viruses, rotavirus and adenovirus infection in patients with acute gastroenteritis in Jakarta, Indonesia.
abstract: Norwalk-like viruses (NLVs), rotavirus and adenovirus are reportedly responsible from 4 to 42% of non-bacterial acute sporadic gastroenteritis. The incidence of NLVs, adenovirus and rotavirus infections in Indonesia is unclear. A total of 402 symptomatic cases from Indonesian patients with acute gastroenteritis and 102 asymptomatic controls that tested negative for bacteria and parasites were screened for the presence of NLVs, rotavirus and adenovirus using the reverse transcriptase-polymerase chain reaction (RT-PCR), Rotaclone kits and Adenoclone kits. Specific prototype probes were used to ascertain which NLV prototypes were present in the area. NLVs were detected in 45/218 (21%), rotavirus was detected in 170/402 (42%) and adenovirus was detected in 11/273 (4%) samples examined. Genetic analysis of the RT-PCR products using specific prototype probes for NLVs indicated that the prototypes were 42% Taunton agent and 58% Hawaii/Snow Mountain agent. Comparative data on patients showed that the incidence of rotavirus infections was two times greater than the NLVs infections, and that adenovirus infections were the least prevalent. All of the control samples tested were negative for NLVs and adenoviruses, however 8/70 (11%) of the samples were positive for rotaviruses. The high incidence of enteric viral-related infections is a threat among acute diarrheic patients in Jakarta, Indonesia.
authors: U.S. Naval Medical Research Unit No. 2, American Embassy Jakarta, Unit 8132, NAMRU-2, FPO AP, 96520-8132, Indonesia.
authors: D
authors: D
authors: Subekti
authors: M
authors: M
authors: Lesmana
authors: P
authors: P
authors: Tjaniadi
authors: N
authors: N
authors: Safari
authors: E
authors: E
authors: Frazier
authors: C
authors: C
authors: Simanjuntak
authors: S
authors: S
authors: Komalarini
authors: J
authors: J
authors: Taslim
authors: J R
authors: JR
authors: Campbell
authors: B A
authors: BA
authors: Oyofo
mesh_terms: D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000453:epidemiology* / Q000821:virology; D000260:Adenoviruses, Human / Q000302:isolation & purification*; D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000302:isolation & purification; D003968:Diarrhea, Infantile / Q000453:epidemiology / Q000821:virology; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D015994:Incidence; D007214:Indonesia / Q000453:epidemiology; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D029322:Norovirus / Q000302:isolation & purification*; D011891:Rain; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology; D012621:Seasons; D017678:Sex Distribution; D014505:Urban Population
chemical_list: D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic