[Damage to macrophage membranes by exposure to middle-wave ultraviolet radiation].
An influence of middle-wave ultraviolet radiation (lambda max = 306 nm) on plasma membranes of mice peritoneal macrophages was studied by microfluorimetry analysis. It was found that a percentage of cells with damaged plasma membranes in the irradiated macrophage population reliably increased with the UVB dose starting with 6 J/cm2. Irradiation of cells with 4.2 J/cm2 UVR dose which does not cause evident damage to plasma membranes led to the latent damage which was detected by treatment with detergent digitonin (4.5 micrograms/ml).
Pirutin
S K
SK
Lomonosov Moscow State University, Biology Faculty, Biophysical Department, Moscow, 119899 Russia. pirutin@aport.ru
Turovetskiĭ
V B
VB
Kudriashov
Iu B
IuB
Rubin
A B
AB
D000818:Animals; D002462:Cell Membrane / Q000528:radiation effects*; D002478:Cells, Cultured; D003627:Data Interpretation, Statistical; D004072:Digitonin / Q000494:pharmacology; D005470:Fluorometry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D017737:Macrophages, Peritoneal / Q000528:radiation effects*; D051379:Mice; D011829:Radiation Dosage; D014466:Ultraviolet Rays
D007202:Indicators and Reagents; D004072:Digitonin