title: Examination of the Borrelia burgdorferi transcriptome in Ixodes scapularis during feeding.
abstract: Borrelia burgdorferi gene expression within the guts of engorging Ixodes scapularis ticks was examined by use of differential immunoscreening and differential expression with a customized amplified library. Fourteen chromosomal genes involved in energy metabolism, substrate transport, and signal transduction and 10 (4 chromosomal and 6 plasmid) genes encoding putative lipoproteins and periplasmic proteins were preferentially expressed in engorging ticks. These data demonstrate a new approach to the global analysis of B. burgdorferi genes that are preferentially expressed within the vector during feeding.
authors: Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, L Diagnostics, New Haven, Connecticut 06520, USA.
authors: Sukanya
authors: S
authors: Narasimhan
authors: Felix
authors: F
authors: Santiago
authors: Raymond A
authors: RA
authors: Koski
authors: Brandon
authors: B
authors: Brei
authors: John F
authors: JF
authors: Anderson
authors: Durland
authors: D
authors: Fish
authors: Erol
authors: E
authors: Fikrig
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D015139:Blotting, Southern; D025065:Borrelia burgdorferi / Q000235:genetics* / Q000378:metabolism; D005247:Feeding Behavior; D005784:Gene Amplification; D015870:Gene Expression; D015698:Genomic Library; D018884:Ixodes / Q000382:microbiology* / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger