Transcriptional analysis of the recA gene in Streptomyces rimosus: identification of the new type of promoter.
Using primer-extension analysis we identified two transcription start sites for the recA gene in Streptomyces rimosus. A longer, weak transcript is initiated from the distal SEP promoter that contains a Cheo box like sequence: GAAC-N4-ATTC. However, the major start site of transcription is a G at position -36 and this shorter transcript significantly increases in response to DNA damage by UV-light. The -35 box (TTGTCA) and -10 box (TAGCGT) of the strong recA promoter are only 11 bp apart and this proximal promoter is almost identical to the strong, DNA damage-inducible promoter of Mycobacterium tuberculosis recA gene. We inspected the Streptomyces coelicolor database and found this type of promoter in the upstream regions of many (potentially) UV-inducible genes as well as some other genes/ORFs. Moreover, the DNA sequence between the predicted -35 and -10 boxes is also partially conserved. The consensus sequence for this new type of promoter in Streptomyces is: TTGTCAGTGGC-N6-TAGggT.
Ahel
Ivan
I
Department of Molecular Genetics, Rudjer Bosković Institute, pp180, 10002 Zagreb, Croatia.
Vujaklija
Dusica
D
Mikoc
Andreja
A
Gamulin
Vera
V
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D016384:Consensus Sequence; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics / Q000528:radiation effects; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects; D005798:Genes, Bacterial / Q000528:radiation effects; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic / Q000235:genetics; D011938:Rec A Recombinases / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013302:Streptomyces / Q000235:genetics* / Q000528:radiation effects; D024363:Transcription Initiation Site; D014158:Transcription, Genetic / Q000528:radiation effects; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011938:Rec A Recombinases