Antitumor effect of MAb EMD 55900 depends on EGF-R expression and histopathology.
The proliferative stimulus of the epidermal growth factor (EGF) in human epithelial cells is mediated by its binding to the external domain of the EGF receptor (EGF-R). The purpose of this study was to investigate whether growth arrest of tumors treated with anti-EGF-R MAb (EMD 55900) was dependent on EGF-R expression and distinct histopathologic criteria of those neoplasms. Nine different adenocarcinomas, squamous cell carcinomas and two neoplastic epithelial cell lines (A431 and Detroit 562), which were characterized by high EGF-R expression, were xenotransplanted onto NMRI-nu/nu mice and treated with an anti-EGF-R antibody (EMD 55900). Results revealed that EGF-R expression and distinct histopathologic growth patterns play an important role for the therapeutic effect of the EGF-R antibody treatment. Tumors with high epithelial cellularity and little connective tissue responded to EMD 55900 treatment to a greater degree of growth reduction than tumors with lower cellularity. These results will be helpful for evaluation of patients who would benefit from tumor therapy with anti-EGF-R antibody.
Solbach
Christine
C
Department of Gynecology and Obstetrics, School of Medicine, Johann Wolfgang Goethe University, Theodor-Stern Kai 7, D-60590 Frankfurt am Main, Germany.
Sterner-Kock
Anja
A
Roller
Marc
M
Schnürch
Hans Georg
HG
Stegmüller
Manfred
M
Caspar-Bell
Gudrun
G
Schumm-Draeger
Petra Maria
PM
Kaufmann
Manfred
M
Knecht
Rainald
R
D000230:Adenocarcinoma / Q000628:therapy; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000628:therapy; D002455:Cell Division; D004847:Epithelial Cells / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008024:Ligands; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D008024:Ligands; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D066246:ErbB Receptors