T-cell/periodic acid-Schiff stain: a useful tool in the evaluation of tubulointerstitial infiltrates as a component of renal allograft rejection.
Distinguishing between tubulitis and tubulointerstitial mononuclear cell infiltrates and determining the severity of tubulitis are critical components of diagnosing and grading renal allograft rejection using the 1993 Banff schema, the revised 1997 Banff schema, or the Cooperative Clinical Trials in Transplantation grading system. We describe a novel staining method, the T-PAS stain (CD3 and periodic acid-Schiff), which removes some of the subjectivity in the evaluation of tubulointerstitial infiltrates in renal allograft biopsies. The method simply combines two routine stains, immunoperoxidase staining for T cells (CD3) and periodic acid-Schiff (PAS) staining for tubular basement membrane, on the same section.
Resch
Lothar
L
Department of Pathology, IWK Health Centre and Dalhousie University Faculty of Medicine, Halifax, Nova Scotia, Canada.
Yu
Weiming
W
Fraser
Robert B
RB
Lawen
Joseph G
JG
Acott
Philip D
PD
Crocker
John F S
JF
Wright
James R
JR
D001485:Basement Membrane / Q000378:metabolism / Q000473:pathology; D001707:Biopsy, Needle; D006084:Graft Rejection / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D016030:Kidney Transplantation; D007684:Kidney Tubules / Q000378:metabolism / Q000473:pathology*; D009395:Nephritis, Interstitial / Q000378:metabolism / Q000473:pathology*; D010503:Periodic Acid-Schiff Reaction; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology*