[Comparison of cytogenetic response of human lymphocytes to in vivo and in vitro exposure to low-dose gamma rays. Translocations and dicentrics detected by the FISH technique].
On peripheral lymphocytes of 5 cancer patients undergone wholebody therapeutic irradiation (at daily dose of 10 cGy up to total dose 50 cGy of 60Co gamma-rays) the dose response of unstable and stable chromosomal exchanges detected by FISH was compared with the dose response of the some aberrations in lymphocytes irradiated in vitro. The dose response fitted well to linear function. For dicentrics the lower slope of dose-response curve was found for in vivo irradiated lymphocytes as compared to the dose-response curve obtained for in vitro irradiated lymphocytes of the same patients. No difference between in vivo and in vitro irradiation of lymphocytes was found for translocations. The frequency of translocations increased faster with the dose than the frequency of dicentrics only in lymphocytes irradiated in vivo.
Vorobtsova
I E
IE
Central Research Institute of Roentgenology and Radiology, Ministry of Health of Russa, St. Petersburg, 197758 Russia.
Kanaeva
A Iu
AIu
Timofeeva
N M
NM
Semënov
A V
AV
Zharinov
G M
GM
Darrudi
F
F
Nataradzhan
A T
AT
D000328:Adult; D000368:Aged; D001859:Bone Neoplasms / Q000556:secondary; D002478:Cells, Cultured / Q000528:radiation effects; D016009:Chi-Square Distribution; D002869:Chromosome Aberrations; D002894:Chromosomes, Human, Pair 4 / Q000528:radiation effects; D002898:Chromosomes, Human, Pair 8 / Q000528:radiation effects; D003037:Cobalt Radioisotopes / Q000627:therapeutic use; D020732:Cytogenetic Analysis; D003627:Data Interpretation, Statistical; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000532:radiotherapy; D009369:Neoplasms / Q000235:genetics / Q000532:radiotherapy; D011829:Radiation Dosage; D011879:Radiotherapy Dosage; D014178:Translocation, Genetic; D014916:Whole-Body Irradiation
D003037:Cobalt Radioisotopes