High pressure static fluorescence to study macromolecular structure-function.
Through some typical examples, the high pressure static fluorescence method is described. The potentiality of the intrinsic and extrinsic fluorescence probes are analyzed for structural characterizations. Special attention is given to the use of fluorescence to understand the behavior of enzymatic reactions under high pressure. The application of fluorescence polarization is also presented together with some relevant spectroscopic problems inherent in data interpretation.
Ruan
Kangcheng
K
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai, PR China.
Balny
Claude
C
D005453:Fluorescence; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D011312:Pressure; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013329:Structure-Activity Relationship
D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D011506:Proteins