Toxicities of two freshwater puffers in Taiwan.
Forty-two and twelve specimens of puffers Tetraodon ocellatus and T. nigroviridis were, respectively, collected from the aquaria in Taiwan and determined for toxicity by using tetrodotoxin bioassay. It was found that T. ocellatus contained moderate amounts of toxin in skin and viscera, and the highest toxicity scores were 432 MU/g skin and 212 MU/g viscera. The specimens of T. nigroviridus contained weak amounts of toxin in skin, and the highest toxicity score was 124 MU/g. The toxin was partially purified from the toxic specimens of each species by ultrafiltration using a YM-1 membrane, followed by chromatography on Bio-Gel P-2 column. Analyses by electrophoresis, thin layer chromatography, and high performance liquid chromatography showed that the toxin from both species was composed of tetrodotoxin and anhydrotetrodotoxin.
Shin-Jung
Lin
L
Department of Food Health, Deh Yu College of Nursing and Management, Keelung, Taiwan, ROC.
Liao
Chien-Fu
CF
Arakawa
Osamu
O
Noguchi
Tamao
T
Hwang
Deng-Fwu
DF
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004588:Electrophoresis, Cellulose Acetate; D005517:Foodborne Diseases / Q000517:prevention & control; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012621:Seasons; D012867:Skin / Q000737:chemistry; D013045:Species Specificity; D013624:Taiwan; D023721:Tetraodontiformes / Q000378:metabolism*; D013779:Tetrodotoxin / Q000302:isolation & purification* / Q000633:toxicity*; D023382:Toxicity Tests, Acute; D014781:Viscera / Q000737:chemistry
D013779:Tetrodotoxin