title: Structural basis for the cyclization of the lipopeptide antibiotic surfactin by the thioesterase domain SrfTE.
abstract: Many biologically active natural peptides are synthesized by nonribosomal peptide synthetases (NRPS). Product release is accomplished by dedicated thioesterase (TE) domains, some of which catalyze an intramolecular cyclization to form macrolactone or macrolactam cyclic peptides. The excised 28 kDa SrfTE domain, a member of the alpha/beta hydrolase enzyme family, exhibits a distinctive bowl-shaped hydrophobic cavity that hosts the acylpeptide substrate and tolerates its folding to form a cyclic structure. A substrate analog confirms the substrate binding site and suggests a mechanism for substrate acylation/deacylation. Docking of the peptidyl carrier protein domain immediately preceding SrfTE positions the 4'-phosphopantheinyl prosthetic group that transfers the nascent acyl-peptide chain to SrfTE. The structure provides a basis for understanding the mechanism of acyl-PCP substrate recognition and for the cyclization reaction that results in release of the macrolactone cyclic heptapeptide.
authors: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors: Steven D
authors: SD
authors: Bruner
authors: Thomas
authors: T
authors: Weber
authors: Rahul M
authors: RM
authors: Kohli
authors: Dirk
authors: D
authors: Schwarzer
authors: Mohamed A
authors: MA
authors: Marahiel
authors: Christopher T
authors: CT
authors: Walsh
authors: Milton T
authors: MT
authors: Stubbs
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D003500:Cyclization; D008024:Ligands; D055666:Lipopeptides; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010204:Pantetheine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D010453:Peptide Synthases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010456:Peptides, Cyclic; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008024:Ligands; D055666:Lipopeptides; D010456:Peptides, Cyclic; C009365:surfactin peptide; D010204:Pantetheine; D010453:Peptide Synthases; C069542:surfactin synthetase; C003129:4'-phosphopantetheine