Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine.
Heterologous prime/boost regimens have the potential for raising high levels of immune responses. Here, we report that DNA priming followed by a recombinant modified vaccinia Ankara (rMVA) booster has controlled a highly pathogenic immunodeficiency virus challenge in a Rhesus macaque model. Both the DNA and rMVA components of the vaccine expressed multiple immunodeficiency virus proteins. Two DNA inoculations at 0 and 8 weeks and a single rMVA booster at 24 weeks effectively controlled an intrarectal challenge administered 7 months after the booster. These highly promising findings provide hope that a relatively simple multiprotein DNA/MVA vaccine can help to control the AIDS epidemic.
Amara
Rama Rao
RR
Vaccine Research Center, Yerkes Regional Primate Research Center, Emory University, 954 Gatewood Road NE, Atlanta, GA 30329, USA.
Villinger
Francois
F
Altman
John D
JD
Lydy
Shari L
SL
O'Neil
Shawn P
SP
Staprans
Silvija I
SI
Montefiori
David C
DC
Xu
Yan
Y
Herndon
James G
JG
Wyatt
Linda S
LS
Candido
Maria Angelito
MA
Kozyr
Natalia L
NL
Earl
Patricia L
PL
Smith
James M
JM
Ma
Hak-Ling
HL
Grimm
Bennett D
BD
Hulsey
Michael L
ML
McClure
Harold M
HM
McNicholl
Janet M
JM
Moss
Bernard
B
Robinson
Harriet L
HL
D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D018791:CD4 Lymphocyte Count; D004353:Drug Evaluation, Preclinical; D007111:Immunity, Cellular; D018928:Immunity, Mucosal; D007115:Immunization Schedule; D007117:Immunization, Secondary; D007156:Immunologic Memory; D007413:Intestinal Mucosa / Q000276:immunology* / Q000821:virology; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D012007:Rectum; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology / Q000627:therapeutic use; D014616:Vaccinia virus / Q000276:immunology; D019562:Viral Load; D014774:Virulence
D016915:AIDS Vaccines; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic