Rapid diagnosis of M. tuberculosis using a piezoelectric immunosensor.
A piezoelectric immunosensor was developed for detecting Mycobacteria Tuberculosis (M. TB). Protein A was modified onto a silver-coated piezoelectric quartz-crystal sensor. Antibodies could be bond onto the crystal surface in an ordered orientation through protein A. An impendence analyzer was employed to record the admittance-frequency curve. The experimental results showed a successful and less-rigid bonding of protein A and antibodies. Compared with other traditional detection methods of TB, the method suggested in this paper was sensitive, selective and effective. The nonspecific response was limited by using control antibody-rabbit anti-honey bee venom (anti-HBV). A glycine-HCI buffer solution (pH = 2.4) was used to release antibodies from a crystal coated with protein A, and 0.5 mg/mL anti-TB was used to remove TB from a crystal that was bonded by an antibody. Good reusability was exhibited. Spike samples of sputum and saliva from normal people with and without adding M. tuberculosis were diagnosed using the proposed method. Good results were obtained.
He
Fengjiao
F
Department of Chemistry and Chemical Engineering, Hunan University, Changsha, P R China. Fengjiaoh@hotmail.com
Zhang
Liude
L
D000907:Antibodies, Bacterial / Q000378:metabolism; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008567:Membranes, Artificial; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011791:Quartz; D015203:Reproducibility of Results; D012463:Saliva / Q000382:microbiology; D012680:Sensitivity and Specificity; D012834:Silver; D013183:Sputum / Q000382:microbiology; D013205:Staphylococcal Protein A / Q000276:immunology; D014376:Tuberculosis / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D008567:Membranes, Artificial; D013205:Staphylococcal Protein A; D011791:Quartz; D012834:Silver