title: Immunisation of dairy cattle with recombinant Streptococcus uberis GapC or a chimeric CAMP antigen confers protection against heterologous bacterial challenge.
abstract: The gapC genes, encoding the cell surface-associated GapC proteins of S. uberis and S. agalactiae, have been cloned and sequenced. To identify potential vaccine candidates against S. uberis-induced bovine mastitis, lactating dairy cows were vaccinated with either (6 x His)GapC of S. uberis or S. dysgalactiae, or with a chimeric CAMP-factor antigen, CAMP-3. For 7 days following heterologous challenge with S. uberis, milk somatic cell counts were determined to assess differences in the severity of mastitis between vaccinates and an unvaccinated control group. Vaccination with S. uberis (6 x His)GapC or CAMP-3 resulted in a significant reduction in inflammation on several days post-challenge, most significantly for the former antigen. Inflammation was not reduced in S. dysgalactiae (6 x His)GapC vaccinates, suggesting that it does not confer cross-species protection.
authors: Veterinary Infectious Disease Organisation, University of Saskatchewan, Saskatoon, Canada.
authors: Michael C
authors: MC
authors: Fontaine
authors: Jose
authors: J
authors: Perez-Casal
authors: Xin Ming
authors: XM
authors: Song
authors: Jim
authors: J
authors: Shelford
authors: Philip J
authors: PJ
authors: Willson
authors: Andrew A
authors: AA
authors: Potter
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005260:Female; D015870:Gene Expression; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D008414:Mastitis, Bovine / Q000382:microbiology / Q000517:prevention & control*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D011995:Recombination, Genetic; D022541:Streptococcal Vaccines / Q000235:genetics / Q000276:immunology*; D013291:Streptococcus / Q000235:genetics / Q000276:immunology*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C039092:CAMP protein, Streptococcus; D004269:DNA, Bacterial; C469817:GapC protein, Streptococcus; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; D022541:Streptococcal Vaccines; D014614:Vaccines, Synthetic