title: PCR-based detection of resistance to acetyl-CoA carboxylase-inhibiting herbicides in black-grass (Alopecurus myosuroides Huds) and ryegrass (Lolium rigidum gaud).
abstract: A simple method based upon allele-specific PCR was developed to detect an isoleucine-leucine substitution in the gene encoding chloroplastic acetyl-coenzyme A carboxylase (ACCase) in two gramineous weeds: Lolium rigidum Gaud and Alopecurus myosuroides Huds. Analysis of 1800 A myosuroides and 750 L rigidum seedlings showed that the presence of ACCase leucine allele(s) conferred cross-resistance to the cyclohexanedione herbicide cycloxydim and to the aryloxyphenoxypropionate herbicides fenoxaprop-P-ethyl and diclofop-methyl. Seedlings containing ACCase leucine allele(s) could be either sensitive or resistant to the aryloxyphenoxypropionate herbicides haloxyfop-P-methyl and clodinafop-propargyl. Successful detection of resistant plants in a field population of A myosuroides was achieved using this PCR assay. Using it with basic molecular biology laboratory equipment, the presence of resistant leucine ACCase allele(s) can be detected within one working day.
authors: Institut National de la Recherche Agronomique, Laboratoire de Malherbologie et Agronomie, BP 86510, 21065 Dijon, France. delye@dijon.inra.fr
authors: Christophe
authors: C
authors: Délye
authors: Annick
authors: A
authors: Matéjicek
authors: Jacques
authors: J
authors: Gasquez
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors*; D000483:Alleles; D019943:Amino Acid Substitution; D001681:Biological Assay; D004351:Drug Resistance / Q000235:genetics; D006540:Herbicides / Q000378:metabolism* / Q000633:toxicity; D008129:Lolium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020125:Mutation, Missense; D006109:Poaceae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011422:Propionates / Q000378:metabolism / Q000633:toxicity; D011725:Pyridines / Q000378:metabolism / Q000633:toxicity; D012680:Sensitivity and Specificity
chemical_list: D006540:Herbicides; D011422:Propionates; D011725:Pyridines; C054176:haloxyfop; D000103:Acetyl-CoA Carboxylase; C400760:clodinafop