title: The use of a highly sulfated cyclodextrin for the simultaneous chiral separation of amphetamine-type stimulants by capillary electrophoresis.
abstract: We investigated the simultaneous chiral separation of nine amphetamine type stimulants (dl-norephedrine, dl-norpseudoephedrine, dl-ephedrine, dl-pseudoephedrine, dl-amphetamine, dl-methamphetamine, dl-methylenedioxyamphetamine (MDA), dl-methylenedioxymethamphetamine (MDMA), and dl-methylenedioxyethylamphetamine (MDEA)) by capillary electrophoresis using highly sulfated gamma-cyclodextrin (SU(XIII)-gamma-CD) as a chiral selector. Three different approaches using SU(XIII)-gamma-CD with 50 mM phosphate background electrolyte were designed; (I) high CD concentration (10 mM SU(XIII)-gamma-CD) at neutral pH (pH 7.0) in the normal polarity mode, (II) low CD concentration (1.0 mM) at low pH (pH 2.6) in the normal polarity mode and (III) high CD concentration at low pH (pH 2.6) in the reversed-polarity mode. In mode (II), the effects of adding three neutral CDs (beta-CD, dimethyl-beta-CD and hydroxypropyl-beta-CD) were also investigated. The best separation was obtained after optimizing mode (III) as follows: run buffer of 10 mM SU(XIII)-gamma-CD with 50 mM phosphate background electrolyte at pH 2.6, applied voltage of -12 kV and capillary temperature of 15 degrees C.
authors: National Research Institute of Police Science, Chiba, Japan. togawa@nrips.go.jp
authors: Yuko T
authors: YT
authors: Iwata
authors: Agnes
authors: A
authors: Garcia
authors: Tatsuyuki
authors: T
authors: Kanamori
authors: Hiroyuki
authors: H
authors: Inoue
authors: Tohru
authors: T
authors: Kishi
authors: Ira S
authors: IS
authors: Lurie
mesh_terms: D018663:Adrenergic Agents / Q000302:isolation & purification; D000662:Amphetamines / Q000302:isolation & purification*; D000697:Central Nervous System Stimulants / Q000302:isolation & purification*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D013237:Stereoisomerism; D013431:Sulfates / Q000737:chemistry / Q000494:pharmacology; D013696:Temperature; D047408:gamma-Cyclodextrins
chemical_list: D018663:Adrenergic Agents; D000662:Amphetamines; D000697:Central Nervous System Stimulants; D003505:Cyclodextrins; D013431:Sulfates; D047408:gamma-Cyclodextrins; C023792:gamma-cyclodextrin