Flow injection spectrophotometric determination of adrenaline in pharmaceutical formulations using a solid-phase reactor containing lead(IV) dioxide immobilized in a polyester resin.
A flow injection spectrophotometric procedure is proposed for determining adrenaline in pharmaceutical formulations. In this work, the adrenaline in acetate buffer reacts with a solid-phase reactor containing lead(IV) dioxide immobilized in a polyester resin and the adrenochrome yielded was continuously monitored at 486 nm. The analytical curve was linear in the adrenaline concentration range from 0.1 to 0.8 mmol l(-1) with a detection limit of 8 x 10(-3) mmol l(-1). Recoveries of 96.5-105% and relative standard deviation of 0.2% for a solution containing 0.4 mmol l(-1) adrenaline (n = 10) were obtained. The analytical frequency was 130 determinations per hour and the results obtained for adrenaline in pharmaceutical formulations using this procedure and those obtained using a pharmacopoeia procedure are in agreement at the 95% confidence level.
Teixeira
Marcos F S
MF
Departamento de Química, Centro de Ciências Exatas e de Tecnologia, Universidade Federal de São Carlos, SP, Brazil.
Marcolino-Júnior
Luiz H
LH
Fatibello-Filho
Orlando
O
D000323:Adrenochrome / Q000032:analysis / Q000737:chemistry; D004837:Epinephrine / Q000032:analysis* / Q000737:chemistry; D017022:Flow Injection Analysis / Q000295:instrumentation*; D007202:Indicators and Reagents; D007854:Lead / Q000737:chemistry*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D010316:Particle Size; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D015203:Reproducibility of Results; D012117:Resins, Synthetic / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods
D007202:Indicators and Reagents; D010087:Oxides; D004364:Pharmaceutical Preparations; D011091:Polyesters; D012117:Resins, Synthetic; D007854:Lead; C047365:lead oxide; D000323:Adrenochrome; D004837:Epinephrine