title: Molecular cloning and characterization of the mouse reticulon 3 cDNA.
abstract: cDNA of mouse reticulon 3 (mRTN3) was cloned. The cloned cDNA is 1745 bp long and contains an open reading frame of 237 amino acids for a 30 kDa protein. The gene was mapped at band B of chromosome 19 by FISH. Two altematively spliced transcripts, 3.4 and 2.3 kb, of mRTN3 were found by Northem blot analysis. Both transcripts were expressed in many tissues and embryos and the highest expression of the 3.4 kb-transcript was observed in the brain, especially in neurons. The expression of 30 kDa-mRTN3 protein was also greatest in the brain. Both N and C-termini of mRTN3 faced the cytosol, indicating that they may recruit other proteins to the endoplasmic reticulum.
authors: Department of Virology, Kurume University School of Medicine, Fukuoka, Japan.
authors: Nobuyuki
authors: N
authors: Hamada
authors: Jun
authors: J
authors: Iwahashi
authors: Kentaro
authors: K
authors: Suzuki
authors: Hidenao
authors: H
authors: Ogi
authors: Takahito
authors: T
authors: Kashiwagi
authors: Koyu
authors: K
authors: Hara
authors: Michiko
authors: M
authors: Toyoda
authors: Tatsuo
authors: T
authors: Yamada
authors: Tetsuya
authors: T
authors: Toyoda
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C482720:Rtn3 protein, mouse