Carp, a cardiac ankyrin-repeated protein, and its new homologue, Arpp, are differentially expressed in heart, skeletal muscle, and rhabdomyosarcomas.
Arpp, a protein containing an ankyrin repeat domain, PEST sequence, and proline-rich region, is a novel ankyrin-repeated protein highly homologous to Carp, which is proposed to be the putative genetic marker for cardiac hypertrophy. In this study, we comparatively analyzed expression of Arpp and Carp protein in skeletal and cardiac muscles and rhabdomyosarcomas (RMSs). In adult skeletal muscle, Arpp was preferentially expressed in the nucleus and cytoplasm of type I fibers, whereas Carp was barely detectable in skeletal muscle. On the other hand, in adult cardiac muscle, interestingly, Arpp was expressed in ventricles mostly, whereas Carp was expressed throughout the atrium and ventricle. Furthermore, although Carp was identified in fetal heart at 11 developmental weeks, Arpp was very low or undetectable in these fetal hearts. These results suggest that Arpp and Carp are differentially expressed and function in both skeletal and cardiac muscle of fetus and adult. We found that Arpp expression was induced during the differentiation of C2C12 cells in vitro, suggesting that Arpp-expression may be associated with the differentiation stage during myogenesis. Both Arpp and Carp were found to be expressed in all of the RMS cases studied. Because the expression patterns of Arpp in RMS were different from those of muscle actin or desmin, Arpp may be detectable in RMS cases that do not express other existing RMS markers.
Ishiguro
Naoko
N
Department of Molecular Biology, Tottori University, Yonago, Japan.
Baba
Takeshi
T
Ishida
Tsuyoshi
T
Takeuchi
Kengo
K
Osaki
Mitsuhiko
M
Araki
Nobuhito
N
Okada
Eikichi
E
Takahashi
Satsuki
S
Saito
Masahiro
M
Watanabe
Mari
M
Nakada
Chisato
C
Tsukamoto
Yoshiyuki
Y
Sato
Kenzo
K
Ito
Kinji
K
Fukayama
Masashi
M
Mori
Shigeo
S
Ito
Hisao
H
Moriyama
Masatsugu
M
D000199:Actins / Q000032:analysis; D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002454:Cell Differentiation; D002460:Cell Line; D002648:Child; D003893:Desmin / Q000032:analysis; D005260:Female; D005318:Fetal Heart / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D007890:Leiomyosarcoma / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D018657:Muscle Fibers, Slow-Twitch / Q000737:chemistry; D009124:Muscle Proteins; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000166:cytology; D009130:Muscle, Smooth / Q000737:chemistry; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009211:Myoglobin / Q000032:analysis; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D012208:Rhabdomyosarcoma / Q000378:metabolism* / Q000473:pathology; D017386:Sequence Homology, Amino Acid
C491304:ANKRD1 protein, human; C432364:ANKRD2 protein, human; D000199:Actins; D000906:Antibodies; D003893:Desmin; D009124:Muscle Proteins; D009211:Myoglobin; D009687:Nuclear Proteins; D012097:Repressor Proteins