Engineered Listeria monocytogenes as an AIDS vaccine.
Listeria monocytogenes (Lm) is an attractive vector to elicit T cell immunity because it infects antigen-presenting cells and because infection originates at the mucosa. Lm expressing HIV gag elicits sustained high levels of gag-specific CTL in mice. Since Lm causes disease in immunocompromised hosts, a highly attenuated strain of Lm that requires D-Ala for viability was produced. Attenuated bacteria expressing HIV-1 gag (Lmdd-gag) are as efficient as wild-type recombinants at stimulating gag-specific murine CTL when administered with D-Ala and at boosting human CTL in vitro. Lmdd-gag immunization protects mice from vaccinia-gag challenge and induces mucosal CTL, even after systemic immunization.
Lieberman
Judy
J
The Center for Blood Research and Department of Pediatrics, Harvard Medical School, 800 Huntington Avenue, Boston, MA 02115, USA. lieberman@cbr.med.harvard.edu
Frankel
Fred R
FR
D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology / Q000633:toxicity; D000328:Adult; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology / Q000633:toxicity; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D015750:Genes, gag; D015754:Genes, nef; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D018928:Immunity, Mucosal; D007231:Infant, Newborn; D008089:Listeria monocytogenes / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D011247:Pregnancy; D012449:Safety; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014613:Vaccines, Attenuated / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000633:toxicity; D014616:Vaccinia virus / Q000276:immunology
D016915:AIDS Vaccines; D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic