Over-expression of K-FGF or bFGF results in altered expression of matrix metalloproteinases: correlations with malignant progression and cellular invasion.
Matrix metalloproteinase (MMP) expression was investigated in NIH-3T3 fibroblasts that secrete K-FGF and in NIH-3T3 cells which express chimeric bFGF with a signal sequence targeting bFGF to the secretory pathway. Correlations between altered MMPs' and other proteases' expression and malignant potential were determined. Correlations between the expression of MMPs and the invasion ability of K-FGF and bFGF over-expressing cells were also determined. The resulting correlation between alterations in proteases and malignant progression supports a model which suggests that growth factor modulation of protease expression is part of the altered growth regulatory program associated with cellular transformation and malignant progression.
Lo
James
J
Department of Laboratory Medicine and Pathobiology, St. Michael's Hospital and University of Toronto, Canada M5B 1A6.
Hurta
Robert A R
RA
D016475:3T3 Cells; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000235:genetics*; D051519:Fibroblast Growth Factor 4; D005346:Fibroblast Growth Factors / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D020782:Matrix Metalloproteinases / Q000235:genetics*; D051379:Mice; D009361:Neoplasm Invasiveness / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured
C496446:Fgf4 protein, mouse; D051519:Fibroblast Growth Factor 4; D011518:Proto-Oncogene Proteins; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors; D020782:Matrix Metalloproteinases