title: Expression of CD44v5 and -v6 in Barrett's carcinoma is not increased compared to that in nondysplastic Barrett's mucosa.
abstract: Barrett's adenocarcinoma currently shows the highest increase in the incidence of all malignant tumors. Reliable molecular markers to identify Barrett's patients at risk are still missing. Our own results demonstrate that the expression of CD44v6 correlates with the development of dysplasia in colorectal neoplasms. Therefore, we examined the expression of CD44 variants v5 and v6 in normal esophageal mucosa, non-dysplastic Barrett's mucosa, and Barrett's carcinoma. mRNA from biopsy specimens of patients with Barrett's esophagus (n = 19) or Barrett's carcinoma (n = 15) and patients without esophageal diseases (controls; n = 9) were extracted and used as templates for cDNA synthesis. CD44 variants were detected by RT-PCR with primers hybridizing with CD44 sequences up- and downstream of variable exons. CD44v6 expression was found in 36 of 56 biopsy specimens (64%) of non-dysplastic Barrett's mucosa, in 100% of squamous epithelium, and in none of the gastric mucosa specimens. Eleven of 15 specimens (73%) of Barrett's carcinoma tested positive for v6 expression. The identification of v5 expression did not give additional information. There was no correlation between CD44v5 or -v6 expression and staging or grading of the tumors. Expression of CD44v5 and -v6 seems to be independent of the development of cancer in Barrett's mucosa.
authors: Department of Internal Medicine II, Saarland-University, D-66421 Homburg/Saar, Germany.
authors: Markus
authors: M
authors: Menges
authors: Reinhild
authors: R
authors: Goebel
authors: Werner
authors: W
authors: Pueschel
authors: Martin
authors: M
authors: Zeitz
authors: Andreas
authors: A
authors: Stallmach
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000276:immunology*; D017398:Alternative Splicing; D000940:Antigenic Variation; D001471:Barrett Esophagus / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000276:immunology*; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics*; D009092:Mucous Membrane / Q000276:immunology / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C113800:CD44v5 antigen; C112697:CD44v6 antigen; D018076:DNA, Complementary; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D012333:RNA, Messenger; D012334:RNA, Neoplasm