title: Gizzerosine-induced histopathological lesions in broiler chicks.
abstract: 1. The aim of this study was to investigate pathomorphological changes in broiler chicks fed with different doses of gizzerosine, a substance produced during the heat treatments of fish meal. 2. The experiment was carried out in Ross broiler chicks which were divided into three groups: group A received 100% of non-medicated commercial mash for broiler chicks. During an experimental 5-d period, 50% of commercial mash was replaced with unheated fish meal (0.65 ppm gizzerosine) in group B and in group C with heated fish meal (1.15 ppm gizzerosine). Fourteen chicks from each group were killed every day. Samples of gastrointestinal and lymphoid organs, lung, pancreas, liver, brain and kidney tissue were sampled for histopathological analysis. Organs were embedded in paraffin and stained with hematoxylin-eosin stain and using periodic acid-Schiff reagent (PAS) and Sudan III (frozen sections). 3. Necropsy did not reveal notable differences between treated groups. There were no significant histopathological changes in immunocompetent organs nor in the lungs, the pancreas, the kidney or the brain. Sharply demarcated multiple vacuoles were observed in the myocardium in group C toward the end of the experiment. In group C, the prevalent changes in the gizzard and the proventriculus were slight to severe cuticle erosions and oedema of the lamina propria with or without multiple vacuoles, respectively, towards the end of the experiment. The most prominent changes toward the end of the experiment were dispersed cell vacuolisation in duodenal, jejunual, ileal and caecal lamina propria in group C. 4. In conclusion, it should be emphasised that extra-gizzard gizzerosine-induced lesions are probably not mediated by H2-receptor stimulation, but could be a consequence of cellular hypoxia.
authors: Poultry Center, Croation Veterinary Institute, Zagreb.
authors: M
authors: M
authors: Tisljar
authors: Z
authors: Z
authors: Grabarević
authors: B
authors: B
authors: Artuković
authors: Z
authors: Z
authors: Simec
authors: P
authors: P
authors: Dzaja
authors: D
authors: D
authors: Vranesić
authors: A
authors: A
authors: Bauer
authors: M
authors: M
authors: Tudja
authors: V
authors: V
authors: Herak-Perković
authors: P
authors: P
authors: Juntes
authors: M
authors: M
authors: Pogacnik
mesh_terms: D000821:Animal Feed; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D004386:Duodenum / Q000187:drug effects / Q000473:pathology; D005260:Female; D005394:Fish Flour / Q000009:adverse effects*; D005895:Gizzard, Avian / Q000187:drug effects / Q000473:pathology; D006321:Heart / Q000187:drug effects; D007093:Imidazoles / Q000378:metabolism / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000473:pathology
chemical_list: D007093:Imidazoles; C045114:gizzerosine