Sensor-based measurements of the role and interactions of free radicals in cellular systems.
Direct real-time electrochemical measurements have offered new insight into the importance of free radical interplay in a number of cell culture and in vivo models of neurodegenerative processes. This review highlights investigations carried out in this laboratory of real-time superoxide and nitric oxide free radical generation, and presents evidence of complex inter-relationships between these species. These include: a novel function for astrocytic nitric oxide synthase in controlling neuronal nitric oxide availability; and the demonstration that extracellular superoxide flux can lead to the generation of NO by glial cells. The possible consequences of these interactions are discussed.
McNeil
Calum J
CJ
Centre for Nanoscale Science and Technology, The Medical School, University of Newcastle upon Tyne, UK. calum.mcneil@ncl.ac.uk
Manning
Philip
P
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002478:Cells, Cultured; D004563:Electrochemistry; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D017628:Microglia / Q000166:cytology / Q000201:enzymology; D009474:Neurons / Q000166:cytology* / Q000201:enzymology*; D009569:Nitric Oxide / Q000378:metabolism
D005609:Free Radicals; D009569:Nitric Oxide