Improvement of rat liver function by energy repletion after the preservation period: implications for hepatic graft management.
We very recently showed (using a blood-free perfusion model) that cold preservation sensitized rat hepatocyte functions to rewarming ischemic injury and that the injury can be prevented by repleting high-energy adenylates in the liver by short-term oxygenated warm reperfusion. Here we investigated whether short-term reperfusion after the preservation period can improve hepatic graft function in a blood reperfusion model. Eighteen-hour cold-preserved rat livers either untreated (Group A) or pretreated by 30-min oxygenated warm reperfusion after preservation (Group B) were subjected to 20-min ischemic rewarming and then reperfused with blood. Livers in Group B compared to Group A exhibited approx. three times increased bile production and bromosulfophthalein excretion, nearly 7-fold decreased swelling, and 1.2-fold improved blood flow. These results suggest that repletion of the energy by short-term oxygenated reperfusion after prolonged preservation may improve markedly initial hepatic graft function.
Kukan
M
M
Laboratory of Perfused Organs, Institute of Preventive and Clinical Medicine, Limbová 14, 83301 Bratislava, Slovakia.
Vajdová
K
K
Lutterová
M
M
Kristek
F
F
Kebis
A
A
Kuba
D
D
Horecký
J
J
D000241:Adenosine; D000255:Adenosine Triphosphate / Q000378:metabolism; D000493:Allopurinol; D000818:Animals; D001646:Bile / Q000502:physiology; D003080:Cold Temperature; D004734:Energy Metabolism; D005978:Glutathione; D006358:Hot Temperature; D066298:In Vitro Techniques; D007328:Insulin; D008099:Liver / Q000502:physiology* / Q000648:ultrastructure; D016031:Liver Transplantation / Q000473:pathology / Q000502:physiology*; D008297:Male; D008854:Microscopy, Electron; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D009929:Organ Size; D010100:Oxygen / Q000378:metabolism; D010477:Perfusion; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D000255:Adenosine Triphosphate; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D010100:Oxygen