Prostaglandin transporter PGT is expressed in cell types that synthesize and release prostanoids.
PGT is a broadly expressed transporter of prostaglandins (PGs) and thromboxane that is energetically poised to take up prostanoids across the plasma membrane. To gain insight into the function of PGT, we generated mouse monoclonal antibody 20 against a portion of putative extracellular loop 5 of rat PGT. Immunoblots of endogenous PGT in rat kidney revealed a 65-kDa protein in a zonal pattern corresponding to PG synthesis rates (papilla congruent with medulla > cortex). Immunocytochemically, PGT in rat kidneys was expressed in glomerular endothelial and mesangial cells, arteriolar endothelial and muscularis cells, principal cells of the collecting duct, medullary interstitial cells, medullary vasa rectae endothelia, and papillary surface epithelium. Proximal tubules, which are known to take up and metabolize PGs, were negative. Immunoblotting and immunocytochemistry revealed that rat platelets also express abundant PGT. Coexpression of the PG synthesis apparatus (cyclooxygenase) and PGT by the same cell suggests that prostanoids may undergo release and reuptake.
Bao
Yi
Y
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Pucci
Michael L
ML
Chan
Brenda S
BS
Lu
Run
R
Ito
Shigekazu
S
Schuster
Victor L
VL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000918:Antibody Specificity; D017920:Antiporters / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D009865:Oocytes / Q000737:chemistry / Q000378:metabolism; D009928:Organ Specificity; D027361:Organic Anion Transporters; D011453:Prostaglandins / Q000378:metabolism*; D051381:Rats; D014162:Transfection; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D017920:Antiporters; D004268:DNA-Binding Proteins; D027361:Organic Anion Transporters; D011453:Prostaglandins; C503526:SLCO2A1 protein, human; C503527:Slco2a1 protein, mouse; C503528:Slco2a1 protein, rat