Characterization of a novel killer cell lectin-like receptor (KLRH1) expressed by alloreactive rat NK cells.
NK cells have the ability to recognize and kill MHC-mismatched hemopoietic cells. In the present study, strain-specific differences in the rat NK allorecognition repertoire were exploited to generate Abs against receptors that may be involved in allogeneic responses. A mAb termed STOK9 was selected, and it reacted with subsets of NK cells and NKR-P1(+) T cells from certain rat strains possessing highly alloreactive NK cells. The STOK9(+) NK subset was broadly alloreactive and lysed Con A lymphoblast targets from a range of MHC-mismatched strains. The mAb STOK9 precipitated a 75-kDa dimeric glycoprotein from NK lysates. Expression cloning revealed that each monomer consisted of 231 aa with limited homology to other previously characterized killer cell lectin-like receptors (KLRs). This glycoprotein therefore constitutes a novel KLR branch, and it has been termed KLRH1. A gene in the central region of the natural killer gene complex on rat chromosome 4 encodes KLRH1. A mouse homolog appears to be present as deduced from analyses of genomic trace sequences. The function of KLRH1 is unknown, but it contains an immunoreceptor tyrosine-based inhibitory motif, suggesting an inhibitory function. The MHC haplotype of the host appears to influence KLRH1 expression, suggesting that it may function as an MHC-binding receptor on subsets of NK cells and T lymphocytes.
Naper
Christian
C
Department of Anatomy, University of Oslo, and Institute of Immunology, Rikshospitalet, University Hospital, N-0027 Oslo, Norway.
Hayashi
Shigenari
S
Løvik
Guro
G
Kveberg
Lise
L
Niemi
Eréne C
EC
Rolstad
Bent
B
Dissen
Erik
E
Ryan
James C
JC
Vaage
John T
JT
D000595:Amino Acid Sequence; D000818:Animals; D000950:Antigens, Ly; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D019281:Dimerization; D006239:Haplotypes / Q000276:immunology; D006825:Hybridomas; D007519:Isoantigens / Q000235:genetics / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism*; D037102:Lectins / Q000096:biosynthesis / Q000737:chemistry; D037181:Lectins, C-Type; D016131:Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D008285:Major Histocompatibility Complex / Q000235:genetics / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D055630:Receptors, NK Cell Lectin-Like; D017386:Sequence Homology, Amino Acid
D000950:Antigens, Ly; D007519:Isoantigens; C456699:Klrh1 protein, rat; D037102:Lectins; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D055630:Receptors, NK Cell Lectin-Like