Development and optimization of organic acid analysis in tobacco with ion chromatography and suppressed conductivity detection.
With the aid of a central composite face-centered design, an ion chromatographic method was developed and optimized for analyzing organic acids in tobacco. A Dionex-100 ion chromatograph with an ion suppressor and a conductivity detector, and a Bio-Rad Aminex HPX-87H column were employed. Only 13 analyses were required to optimize two factors: column temperature and eluent strength. Two sets of optimal conditions for separating nine acids were found: 1.8 mM HFBA eluent and 42 degrees C column temperature, and 0.8 mM HFBA eluent and 50 degrees C column temperature. The flow-rate was 0.6 ml min(-1) and the analysis time was 18 min or less. A sample preparation procedure included extraction of 2 g ground tobacco with 100 ml of 5 mM sulfuric acid solution for 3 h, filtration of the extract, and dilution of the filtrate 10-fold with deionized water.
Qiu
Jinshu
J
United States Tobacco Manufacturing Limited Partnership, Nashville, TN 37203, USA. qiujinshu@yahoo.com
Jin
Xiaohong
X
D000143:Acids / Q000032:analysis*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D009930:Organic Chemicals / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014026:Nicotiana / Q000737:chemistry*
D000143:Acids; D009930:Organic Chemicals