Effect of gravity on lymphocyte proliferation.
Reports on postflight examination of lymphocytes from crew members of soviet and american spaceships show a depression of reactivity towards mitogens in vitro. The purpose of this communication is to present experimental evidence that gravity can interfere with lymphocyte activation. Lymphocytes were incubated in the presence of concanavalin A in a centrifuge at 2 and 4 g for four days. This environment causes a significant acceleration of the response to the mitogen. In addition, remarkable differences in the ultrastructure of cells grown at 1 g and 4 g are observed by electron microscopy. This investigation is related to the experiment "Effect of weightlessness on lymphocyte proliferation" experiment which will be performed during the first Spacelab mission.
Cogoli
A
A
Laboratorium fur Biochemie, Institut fur Zellbiologie, ETH-Zentrum, Zurich, Switzerland.
Valluchi-Morf
M
M
Bohringer
H R
HR
Vanni
M R
MR
Muller
M
M
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005260:Female; D018471:Hypergravity; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D013154:Spleen / Q000166:cytology; D013936:Thymidine / Q000378:metabolism; D014617:Vacuoles
D003208:Concanavalin A; D004247:DNA; D013936:Thymidine