Expression of transcription factors in the brain of rats with perinatal asphyxia.
No information is available on transcription factors (TF), the main regulators of gene expression, in perinatal asphyxia (PA), and as pathomechanisms in PA are different, data on TFs from ischemia or hypoxia cannot be simply extrapolated to PA, and no studies have been reported to show an expressional pattern or the concerted action of TFs. We, therefore, used a gene-hunting technique, subtractive hybridization, to show sequences different in brains of normoxic and perinatally asphyxiated (10 and 20 min of asphyxia) rats. These subtracted sequences were identified by gene bank and assigned to individual genes. At 10 min of PA the TFs NFI/CAAT-binding protein, NF-kappa-B p65, N-myc, basic helix loop helix protein D82868, and c-myc intron binding protein were upregulated. At 20 min of PA the TFs SOX4 and neuronal death factor were upregulated, whereas the TFs c-maf, PEBP major transcription factor, brn-2, homeodomain protein Af004431, and zinc finger transcriptional factor M65008 were downregulated. The biological meaning of our findings is the demonstration of a pathophysiological pattern of TFs including POU, zinc finger, homeodomain, and basic helix-loop helix motifs in PA, proposing pathomechanisms for brain damage from PA, explaining transcriptional changes in general (as, e.g., NF-kappa-B p65, etc.) or in specific terms (as, e.g., neuronal death factor).
Lubec
B
B
Department of Neonatology, University of Vienna, Austria.
Labudova
O
O
Hoeger
H
H
Kirchner
L
L
Lubec
G
G
D000818:Animals; D000831:Animals, Newborn; D001238:Asphyxia Neonatorum / Q000378:metabolism*; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D004195:Disease Models, Animal; D018257:Helix-Loop-Helix Motifs; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012016:Reference Values; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D016328:NF-kappa B; D012333:RNA, Messenger; D014157:Transcription Factors