A comparison of two skin test methodologies and allergens from two different manufacturers.
BACKGROUND
Skin prick tests (SPTs) are a frequently used method for evaluation of atopy. A variety of standard allergen preparations are available, together with a number of different methods of application.


OBJECTIVE
The objective of this study was to compare SPT reactivity 1) using Soluprick SQ allergens (ALK Allergologisk Laboratorium A/S, Hørsholm, Denmark) and Bayer allergens [Bayer Corporation, West Haven, CT], and 2) using two common methods of application, a standard prick lancet and a Quintest, both produced by Bayer.


METHODS
SPTs were undertaken on 22 adult volunteers (mean age 40 years, 17 female, 5 male). Wheal size was recorded as mean diameters (mm) and area (mm2).


RESULTS
Bayer allergens produced larger mean diameters and areas to dust mites than ALK allergens, with the differences significant when allergens were applied with the lancet. There was a tendency for the ALK cat allergen to produce larger reactions than the Bayer product. The method of application also influenced the wheal size, with the lancet producing significantly larger mean diameters than the Quintest for the histamine and allergens from both manufacturers, except the ALK cat allergen. There were similar differences between methods of application for reactions measured as an area.


CONCLUSIONS
SPTs that use different allergens or different methods of application will not provide comparable assessments of atopy.
Rhodius
Robert
R
Faculty of Medicine, University of Groningen, The Netherlands.
Wickens
Kristin
K
Cheng
Soo
S
Crane
Julian
J
D000328:Adult; D000368:Aged; D000818:Animals; D039741:Antigens, Dermatophagoides; D002415:Cats / Q000276:immunology*; D004890:Erythema / Q000209:etiology / Q000473:pathology; D005188:False Negative Reactions; D005260:Female; D006023:Glycoproteins / Q000008:administration & dosage / Q000009:adverse effects / Q000032:analysis; D006632:Histamine / Q000008:administration & dosage; D006801:Humans; D008297:Male; D008925:Mites / Q000276:immunology*; D012882:Skin Tests / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D014020:Tissue Extracts / Q000008:administration & dosage / Q000009:adverse effects / Q000592:standards
D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D014020:Tissue Extracts; D006632:Histamine; C059296:Fel d 1 protein, Felis domesticus