The effects of osmotic and hydrostatic pressures on macromolecular systems.
Osmotic pressure and hydrostatic pressure can be used effectively to probe the behavior of biologically important macromolecules and their complexes. Using the two techniques requires a theoretical framework as well as knowledge of the more common pitfalls. Both are discussed in this review in the context of several examples.
Kornblatt
Jack A
JA
Enzyme Research Group, Department of Biology, Concordia University, Montreal, QC, Canada. krnbltt@vax2.concordia.ca
Kornblatt
M Judith
MJ
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry*; D003576:Electron Transport Complex IV / Q000737:chemistry*; D006801:Humans; D006874:Hydrostatic Pressure; D046911:Macromolecular Substances; D009696:Nucleic Acids / Q000737:chemistry; D009997:Osmotic Pressure; D010751:Phosphopyruvate Hydratase / Q000737:chemistry*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
D046911:Macromolecular Substances; D009696:Nucleic Acids; D011506:Proteins; D003577:Cytochrome P-450 Enzyme System; D003576:Electron Transport Complex IV; D010751:Phosphopyruvate Hydratase