Kinetic flow-injection determination of hydrogen peroxide by use of iron(III)-catalyzed coloration and its application to the determination of biological substances.
A kinetic flow-injection (FI) method is described for the determination of hydrogen peroxide. This method is based on an iron(III)-catalyzed oxidative coupling of 4-aminoantipyrine with N,N-dimethylaniline by hydrogen peroxide. By measuring the change in the absorbance of the dye formed at 560 nm, 1 x 10(-6) - 6 x 10(-4) M hydrogen peroxide could be determined with a sampling rate of 15 h(-1). The relative standard deviation (n = 30) was 0.8% for 5 x 10(-5) M hydrogen peroxide. There was little interference of the co-existing ions and compounds. After introducing some immobilized enzyme reactors to the FI system, the proposed method allowed the determination of glucose and uric acid ranging from 1 x 10(-6) to 6 x 10(-4) M with relative standard deviations of below 2%. The applicability of the method was demonstrated by determining these substances in serum samples.
Maeuchihara
N
N
Department of Chemistry, University of Tsukuba, Japan.
Nakano
S
S
Kawashima
T
T
D000675:Ampyrone / Q000737:chemistry; D000814:Aniline Compounds / Q000737:chemistry; D001786:Blood Glucose / Q000032:analysis; D002138:Calibration; D002384:Catalysis; D017022:Flow Injection Analysis; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D007202:Indicators and Reagents; D007501:Iron; D007700:Kinetics; D010783:Photometry; D014503:Urate Oxidase / Q000737:chemistry; D014527:Uric Acid / Q000097:blood
D000814:Aniline Compounds; D001786:Blood Glucose; D007202:Indicators and Reagents; D000675:Ampyrone; D014527:Uric Acid; C015157:N,N-dimethylaniline; D006861:Hydrogen Peroxide; D007501:Iron; D005949:Glucose Oxidase; D014503:Urate Oxidase