title: A simple method for focally delivering multiple drugs or ligands to cells.
abstract: A simple venturi-based "spritzer" device is described that is capable of repeatedly and rapidly delivering small volumes of different solutions to cells or tissues. This is particularly advantageous in situations when the appropriate ligand or drug is not known a priori and has to be determined "on the fly" during the limited lifetime of a preparation. The utility of the device is demonstrated for applying odorants to olfactory receptor neurons (ORNs).
authors: Whitney Laboratory, Department of Neuroscience, Center for Smell and Taste, University of Florida, 9505 Ocean Shore Boulevard, Gainesville, FL 32610, USA. doolin@whitney.ufl.edu
authors: Richard E
authors: RE
authors: Doolin
authors: Barry W
authors: BW
authors: Ache
mesh_terms: D000818:Animals; D008024:Ligands; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008845:Microinjections / Q000295:instrumentation* / Q000379:methods*; D008121:Nephropidae; D009812:Odorants; D018034:Olfactory Receptor Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011392:Proline / Q000494:pharmacology
chemical_list: D008024:Ligands; D011392:Proline