title: cDNA cloning of biotransformation enzymes belonging to the cytochrome P450 family in the antennae of the noctuid moth Mamestra brassicae.
abstract: The involvement of cytochrome P450 enzymes in olfaction was demonstrated in vertebrates some time ago. In insects these enzymes are well known for their role in insecticide resistance, but the involvement of P450 in pheromone degradation was only recently demonstrated. Using a PCR strategy, we have isolated two cDNAs from the antennae of the cabbage armyworm Mamestra brassicae - CYP4L4 and CYP4S4 - which encode microsomal P450s. CYP4S4 expression is restricted to the antennae, whereas CYP4L4 is also found in the proboscis and legs. Moreover, the two genes are strongly expressed in one type of sensory unit of the antennae - the sensilla trichodea - which are tuned to the detection of odourants. The putative function of the corresponding enzymes is discussed with regard to their respective expression patterns.
authors: Unité de Phytopharmacie et Médiateurs Chimiques, INRA, France. maibeche@versailles.inra.fr
authors: M
authors: M
authors: Maïbèche-Coisne
authors: E
authors: E
authors: Jacquin-Joly
authors: M C
authors: MC
authors: François
authors: P
authors: P
authors: Nagnan-Le Meillour
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; C460222:cytochrome P-450 CYP4L4 (cabbage armyworm); C460221:cytochrome P-450 CYP4S4 (cabbage armyworm); D003577:Cytochrome P-450 Enzyme System