Expression of anthrax lethal factor gene by osmolyte induction.
The anthrax toxin consists of protective antigen (PA), lethal factor (LF) and edema factor (EF). PA mediates the entry of LF and EF to the cytosol where they exert their effects. Although PA is the major component of the vaccines against anthrax, LF has also been found to play an important role in enhancing protective immunity. We have developed an osmolyte-inducible LF expression system. The protein expression system contributed no additional amino acids to the recombinant LF making it suitable for the human vaccine trials.
Singh
Aparna
A
Centre for Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India.
Singh
Samer
S
Waheed
Syed Mohsin
SM
Khandelwal
Puneet
P
Bhatnagar
Rakesh
R
D000881:Anthrax / Q000382:microbiology* / Q000517:prevention & control; D022122:Anthrax Vaccines; D000942:Antigens, Bacterial; D001408:Bacillus anthracis / Q000235:genetics* / Q000472:pathogenicity; D001427:Bacterial Toxins; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification; D002460:Cell Line; D004926:Escherichia coli; D015964:Gene Expression Regulation, Bacterial; D008264:Macrophages / Q000166:cytology / Q000382:microbiology; D009997:Osmotic Pressure; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification; D014774:Virulence
D022122:Anthrax Vaccines; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D002352:Carrier Proteins; D011993:Recombinant Fusion Proteins; C030325:anthrax toxin