title: Lectin histochemistry of the rat lymph node: visualisation of stroma, blood vessels, sinuses, and macrophages. A contribution to the concept of an immune accessory role of sinus-lining endothelia.
abstract: The lectin Chelidonium majus agglutinin (CMA) was previously shown to visualise endothelia of all blood vessels and those lining sinuses of red pulp, stromal reticular meshwok (RM) and dendritic cells of lymphatic follicles in white pulp of the spleen in rats. The aim of the present study was the analysis of CMA and some other lectins in labelling RM, vascular structures and macrophages in lymph nodes of rats. It appeared that CMA stained the entire RM, dendritic cells, lining cells of sinuses and all types of blood vessels. Sinus-lining cells of lymph nodes were labelled with CMA and mannose-, GalNac-, and sialic acid-specific lectins. Moreover, lymph node macrophages were labelled above all by mannose specific lectins. The broad lectin-binding pattern of sinuses--not observed in rat spleen- and CMA-reactivity of both sinus-lining and dendritic cells corroborates the hypothesis that lymph node sinus-lining endothelia are precursors or a special type of immune accessory cells.
authors: Institute for Anatomy, University Hospital Hamburg-Eppendorf, Hamburg, Germany. duellmann@uke.uni-hamburg.de
authors: Jochen
authors: J
authors: Düllmann
authors: Els J M
authors: EJ
authors: Van Damme
authors: Willy J
authors: WJ
authors: Peumans
authors: Maike
authors: M
authors: Ziesenitz
authors: Udo
authors: U
authors: Schumacher
mesh_terms: D000818:Animals; D028281:Chelidonium; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D007124:Immunoenzyme Techniques; D037102:Lectins; D008198:Lymph Nodes / Q000098:blood supply / Q000166:cytology / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D008297:Male; D037121:Plant Lectins; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D037102:Lectins; D037121:Plant Lectins