Evidence of the presence of IS1245 and IS1311 or closely related insertion elements in nontuberculous mycobacteria outside of the Mycobacterium avium complex.
A PCR assay based on the simultaneous detection of IS1245 and IS1311 was developed and used to determine the host range of these insertion elements. Specific PCR products were observed in Mycobacterium malmoense, Mycobacterium scrofulaceum, and Mycobacterium nonchromogenicum, indicating that IS1245 and IS1311 are not limited to the Mycobacterium avium complex.
Keller
Andreas P
AP
Department of General Pediatrics, Children's Hospital, University of Heidelberg, 69120 Heidelberg, Germany.
Beggs
Marjorie L
ML
Amthor
Beate
B
Bruns
Fabian
F
Meissner
Peter
P
Haas
Walter H
WH
D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D009161:Mycobacterium / Q000145:classification / Q000235:genetics*; D009162:Mycobacterium avium / Q000145:classification / Q000235:genetics; D015359:Mycobacterium scrofulaceum / Q000145:classification / Q000235:genetics; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial