Partially structured state of the functional VH domain of the mouse anti-ferritin antibody F11.
An antibody combining site generally involves the two variable domains, VH from the heavy and VL from the light chain. We expressed the individual VH domain of the mouse anti-human ferritin monoclonal antibody F11. The loss of affinity was not dramatic (K(a)=4.0x10(7) M(-1) versus 8.6x10(8) M(-1) for the parent antibody) and comparable to that previously observed for other VHs. However, the functional VH domain adopted a partially structured state with a significant amount of distorted secondary and compact yet greatly destabilized tertiary structures, as demonstrated by spectroscopic and calorimetric probes. These data provide the first description for a functional antibody domain that meets all the criteria of a partially structured state.
Martsev
Sergey P
SP
Institute of Bio-Organic Chemistry, National Academy of Sciences of Belarus, 220141, Minsk, Byelorussia. martsev@iboch.bas-net.by
Dubnovitsky
Anatoly P
AP
Stremovsky
Oleg A
OA
Chumanevich
Alexander A
AA
Tsybovsky
Yaroslav I
YI
Kravchuk
Zinaida I
ZI
Deyev
Sergey M
SM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000915:Antibody Affinity; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D005293:Ferritins / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000276:immunology; D007142:Immunoglobulin gamma-Chains / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D011487:Protein Conformation; D017510:Protein Folding; D013050:Spectrometry, Fluorescence
D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region; D007142:Immunoglobulin gamma-Chains; D005293:Ferritins