title: Wild-type levels of Spo11-induced DSBs are required for normal single-strand resection during meiosis.
abstract: We have studied the repair of a DNA-DSB created by the VMA1-derived endonuclease in mutants that have different levels of Spo11-DSBs: WT (sae2), few (hop1), and none (spo11-Y135F). In spo11-Y135F and hop1 cells, intrachromosomal repair is more frequent than in WT and sae2 cells. In spo11-Y135F cells there was no chromosome pairing or synapsis and a faster turnover of resected DNA. Compared to WT and sae2 cells, spo11-Y135F and hop1 cells have a greater proportion of long resection tracts. The data suggest that high levels of Spo11-DSBs are required for normal regulation of resection, even at a DSB created by another protein. WT control over resection could be important for directing repair to be interchromosomal, increasing the chance of creating interhomolog connections essential to meiotic segregation.
authors: Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, United Kingdom.
authors: Matthew J
authors: MJ
authors: Neale
authors: Madhu
authors: M
authors: Ramachandran
authors: Edgar
authors: E
authors: Trelles-Sticken
authors: Harry
authors: H
authors: Scherthan
authors: Alastair S H
authors: AS
authors: Goldman
mesh_terms: D019457:Chromosome Breakage; D015825:Chromosomes, Fungal / Q000235:genetics* / Q000648:ultrastructure; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000235:genetics* / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000502:physiology*; D004950:Esterases / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D008540:Meiosis / Q000235:genetics*; D008957:Models, Genetic; D006180:Proton-Translocating ATPases; D011995:Recombination, Genetic / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D017384:Sequence Deletion; D013573:Synaptonemal Complex / Q000502:physiology; D000099257:Meiotic Recombination Protein SPO11
chemical_list: D004271:DNA, Fungal; D004277:DNA, Single-Stranded; D004706:Endodeoxyribonucleases; D004950:Esterases; D006180:Proton-Translocating ATPases; D029701:Saccharomyces cerevisiae Proteins; D000099257:Meiotic Recombination Protein SPO11; C081687:VMA1 protein, S cerevisiae