New simple liquid chromatographic method for the determination of trimethoprim, sulfadiazine and N4-acetylsulfadiazine in plasma of broilers.
A new method for simultaneous quantification of trimethoprim, sulfadiazine and N4-acetylsulfadiazine in plasma of broilers at levels down to 13-16 ng/ml has been developed. Samples were deproteinized with acetonitrile, defatted with hexane, and extracted with dichloromethane. Chromatographic analysis was carried out on a C18 column in the presence of tetrabutylammonium hydrogen sulfate, a competing base, while detection was performed at 240 nm for trimethoprim, and 270 nm for both sulfadiazine and N4-acetylsulfadiazine. Accuracy and precision data showed recoveries and relative standard deviation values better than 87.3% and 3.1%, respectively, for all three analytes. The good analytical characteristics of the method could allow limits of detection in the low ng/ml range to be realised. The method was successfully applied to determine drug concentrations in plasma samples from broilers administered a combination of sulfadiazine and trimethoprim.
Batzias
G C
GC
Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Aristotle University, Thessaloniki, Greece. batzias@vet.auth.gr
Botsoglou
N A
NA
Kotsaki-Kovatsi
V P
VP
Kounenis
G
G
D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood* / Q000493:pharmacokinetics; D002645:Chickens / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013411:Sulfadiazine / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D014295:Trimethoprim / Q000097:blood* / Q000493:pharmacokinetics
D000890:Anti-Infective Agents; D013411:Sulfadiazine; C036312:N(4)-acetylsulfadiazine; D014295:Trimethoprim