The study of nafion/xanthine oxidase/au colloid chemically modified biosensor and its application in the determination of hypoxanthine in myocardial cells in vivo.
A novel hypoxanthine (Hx) microsensor was constructed. In this work, Nafion xanthine oxidase (XOD) and Au colloid were immobilized onto the surface of a Pt microelectrode. The enzyme biosensor displayed a quick and sensitive response to Hx. Under physiological conditions, a low detection limit, with high selectivity and sensitivity for Hx determination were obtained. The oxidation current [investigated using current-time (I-t) plots] was linear with Hx concentration ranging from 2.0 x 10(-7) to 2.0 x 10(-5) mol L(-1) with a calculated detection limit of 1.0 x 10(-7) mol L(-1) (S/N of 3). The biosensor should be promising for in vivo measurement of Hx without interferences and fouling. The change of Hx concentration in cardiac myocytes stimulated by L-arginine (L-Arg) and acetylcholine (Ach) was also studied.
Shi
Guoyue
G
Department of Chemistry, East China Normal University, Shanghai.
Liu
Min
M
Zhu
Min
M
Zhou
Tianshu
T
Chen
Junshui
J
Jin
Litong
L
Jin
Ji-Ye
JY
D015374:Biosensing Techniques; D005465:Fluorocarbon Polymers; D017979:Gold Colloid; D006801:Humans; D019271:Hypoxanthine / Q000032:analysis*; D009206:Myocardium / Q000737:chemistry*; D014969:Xanthine Oxidase
D005465:Fluorocarbon Polymers; D017979:Gold Colloid; D019271:Hypoxanthine; C040402:perfluorosulfonic acid; D014969:Xanthine Oxidase