title: [HIV genomic mutation on antiretroviral therapy].
abstract: BACKGROUND
  To study the characteristics of HIV genomic mutation on antiretroviral therapy.
  
  
  METHODS
  HIV DNA and HIV RNA were detected by using Taq-PCR, RT-PCR, LD-PCR, hybridization, and oligonucleotide 32P- probes against gag, tat, nef, LTRU3, etc. RESULYS: The HIV RNA load in serum declined significantly, CD4 lymphocyte count increased, copies of gag region of HIV DNA decreased, and full-length HIV DNA disappeared. Bands showing gag segments hybridized with 32P-probe appeared negative or less clear in some cases after antiretroviral therapy. However, the regions hybridized with probes Xho-nef and LTR U5 were apparently enhanced and some drawbands were seen.
  
  
  CONCLUSIONS
  HIV gene may experiece obvious mutation on antiretroviral therapy.
authors: Department of Infectious Diseases, the First Clinic Hospital, Beijing Medical University, 100034 Beijing, China.
authors: X
authors: X
authors: Xu
authors: H
authors: H
authors: Xing
authors: J C
authors: JC
authors: Chermannn
mesh_terms: D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D005260:Female; D005814:Genes, Viral / Q000235:genetics*; D006678:HIV / Q000235:genetics*; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation