One-step PCR to distinguish B virus from related primate alphaherpesviruses.
By adding betaine to the PCR mixture, we previously established a PCR method to amplify a DNA segment of the glycoprotein G gene of B virus (BV) derived from a rhesus macaque. We have found that DNA of other BV strains derived from cynomolgus, pigtail, and lion-tailed macaques can also serve as the template in our PCR assay. Under the same conditions no product was obtained with DNA of simian agent 8 of green monkeys and Herpesvirus papio 2 of baboons, or the human herpes simplex viruses types 1 and 2. Thus, this PCR method is useful to discriminate BV from other closely related primate alphaherpesviruses.
Hirano
Makoto
M
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
Nakamura
Shin
S
Mitsunaga
Fusako
F
Okada
Maki
M
Shirahama
Shuya
S
Eberle
Richard
R
D018138:Alphaherpesvirinae / Q000145:classification* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001622:Betaine; D002522:Chlorocebus aethiops; D006566:Herpesviridae Infections / Q000662:veterinary / Q000821:virology*; D006568:Herpesvirus 1, Cercopithecine / Q000145:classification* / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000145:classification / Q000235:genetics; D018258:Herpesvirus 2, Human / Q000145:classification / Q000235:genetics; D006801:Humans; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D010215:Papio; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011323:Primates; D014709:Vero Cells
D001622:Betaine