title: Identifying the major proteome components of Helicobacter pylori strain 26695.
abstract: The whole genome sequences of Helicobacter pylori strain 26695 have been reported. Whole cell proteins of H. pylori strain 26695 cells were obtained and analyzed by two-dimensional electrophoresis, using immobilized pH gradient strips. The most abundant proteins were shown in the region of pI 4.0-9.5 with molecular masses from 10 to 100 kDa. Soluble proteins were precipitated by the use of 0-80% saturated solutions of ammonium sulfate. Soluble proteins precipitated by the 0-40% saturations of ammonium sulfate produced similar spot profiles and their abundant protein spots had acidic pI regions. However, a number of soluble proteins precipitated by more than 60% saturation of ammonium sulfate were placed in the alkaline pI regions, compared to those precipitated by 40% saturation. In addition, we have performed an extensive proteome analysis of the strain utilizing peptide MALDI-TOF-MS. Among the 345 protein spots processed, 175 proteins were identified. The identified spots represented 137 genes. One-hundred and fifteen proteins were newly identified in this study, including DNA polymerase III beta-subunit. These results might provide guidance for the enrichment of H. pylori proteins and contribute to construct a master protein map of H. pylori.
authors: Department of Microbiology, College of Medicine, Gyeongsang National University, Chinju, Kyung-Nam, Korea.
authors: Myung-Je
authors: MJ
authors: Cho
authors: Beong-Sam
authors: BS
authors: Jeon
authors: Jeong-Won
authors: JW
authors: Park
authors: Tae-Sung
authors: TS
authors: Jung
authors: Jae-Young
authors: JY
authors: Song
authors: Woo-Kon
authors: WK
authors: Lee
authors: Yeo-Jeong
authors: YJ
authors: Choi
authors: Sang-Haeng
authors: SH
authors: Choi
authors: Seong-Gyu
authors: SG
authors: Park
authors: Jeong-Uck
authors: JU
authors: Park
authors: Mi-Young
authors: MY
authors: Choe
authors: Seun-Ae
authors: SA
authors: Jung
authors: Eun-Young
authors: EY
authors: Byun
authors: Seung-Chul
authors: SC
authors: Baik
authors: Hee-Shang
authors: HS
authors: Youn
authors: Gyung-Hyuck
authors: GH
authors: Ko
authors: DongBin
authors: D
authors: Lim
authors: Kwang-Ho
authors: KH
authors: Rhee
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D016480:Helicobacter pylori / Q000737:chemistry*; D008970:Molecular Weight; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D020543:Proteome