Modulation of osteoblast function using poly(D,L-lactic acid) surfaces modified with alkylation derivative of chitosan.
Poly(D,L-lactic acid) (PDLLA) was modified with alkylated chitosan (N-butyl chitosan and N-cetyl chitosan), and the effects of modified films on the functions of rat osteoblasts were investigated. The characteristics of surfaces (both modified and control) were examined by water contact angle measurement and electron spectroscopy for chemical analysis (ESCA). Cell morphologies on these surfaces were taken using scanning electron microscopy (SEM). Cell attachment and proliferation were used to assess cell behavior on modified surface and control. MTT assay was used to determined cell viability, and alkaline phosphatase (ALP) activity was taken to evaluate differentiated cell function. Compared with the untreated films, no significant difference in cell attachment of osteoblasts was found on the modified films at a period of 8 h (p > 0.05). However, cell proliferation of N-butyl chitosan rather than N-cetyl chitosan modified PDLLA films was significantly higher than that found on control one (p < 0.05) at the end of the 4th and 7th days. The cell viability of osteoblasts on N-butyl chitosan modified PDLLA films were found higher than that on control (p < 0.05). These results suggested that N-butyl chitosan contributed greater than N-cetyl chitosan when used to modify PDLLA films for improving its biocompatibility.
Cai
Kaiyong
K
Research Institute of Polymeric Materials, Tianjin University, P.R. China.
Liu
Wenguang
W
Li
Fang
F
Yao
Kangde
K
Yang
Zhiming
Z
Li
Xiuqiong
X
Xie
Huiqi
H
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000494:pharmacology*; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D048271:Chitosan; D004396:Coloring Agents / Q000494:pharmacology; D007700:Kinetics; D019344:Lactic Acid / Q000494:pharmacology*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D051381:Rats; D012886:Skull / Q000166:cytology; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D014867:Water
D001672:Biocompatible Materials; D004396:Coloring Agents; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D013778:Tetrazolium Salts; D013844:Thiazoles; D014867:Water; C067347:N-carboxybutyl chitosan; D002686:Chitin; D019344:Lactic Acid; C033616:poly(lactide); C012693:N-acetylchitosan; D048271:Chitosan; D000469:Alkaline Phosphatase; C022616:thiazolyl blue; D010100:Oxygen