Biological significance of proliferation, apoptosis, cytokines, and monocyte/macrophage cells in bone marrow biopsies of 145 patients with myelodysplastic syndrome.
Labeling index (LI), apoptosis, levels of 2 pro-apoptotic cytokines tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta(TGF-beta), and the number of monocyte/macrophage cells that are the likely source of the cytokines were simultaneously measured in plastic-embedded bone marrow (BM) biopsy sections of 145 patients with myelodysplastic syndromes (MDS). TNF-alpha was correlated with TGF-beta (P = .001) and with monocyte/macrophage cells (P = .003). Patients with excess blasts in their marrows had a higher TGF-beta level (P = .01) and monocyte/macrophage number (P = .05). In a linear regression model,TGF-beta emerged as the most significant biological difference between patients who have excess of blasts and those who do not (P = .01). We conclude that in addition to TNF-alpha, TGF-beta also plays a significant role in the initiation and pathogenesis of MDS, and that a more precise definition of its role will likely identify better preventive and therapeutic strategies.
Allampallam
Krishnan
K
Rush Cancer Institute, Rush-Presbyterian-St Luke's Medical Center, Chicago, Illinois 60612, USA.
Shetty
Vilasini
V
Mundle
Suneel
S
Dutt
Diya
D
Kravitz
Howard
H
Reddy
Poluru L
PL
Alvi
Sairah
S
Galili
Naomi
N
Saberwal
Gurveen S
GS
Anthwal
Shalini
S
Shaikh
Maliha W
MW
York
Aaron
A
Raza
Azra
A
D000753:Anemia, Refractory / Q000473:pathology; D000754:Anemia, Refractory, with Excess of Blasts / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000473:pathology*; D002455:Cell Division; D016207:Cytokines / Q000032:analysis*; D005260:Female; D006801:Humans; D015477:Leukemia, Myelomonocytic, Chronic / Q000473:pathology; D008264:Macrophages / Q000473:pathology*; D008297:Male; D009000:Monocytes / Q000473:pathology*; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D012044:Regression Analysis; D016196:S Phase; D016212:Transforming Growth Factor beta / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D016207:Cytokines; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha