The interactions of nucleic acids at elevated hydrostatic pressure.
The application of elevated hydrostatic pressure on the order of a few thousand bar provides insight into the molecular forces responsible for stabilizing the conformations and non-covalent interactions of biological molecules in aqueous solution. In particular, the parameters derived from these studies have enabled researchers to glean information regarding the importance of hydration in the energetics and kinetics of these systems. This review presents data concerned with the application of hydrostatic pressure to study the thermodynamics, kinetics, and structure of nucleic acids and the interactions between nucleic acids and proteins, enzymes, and drugs. These complexes often form extremely stable non-covalent complexes in which electrostatic interactions play an important role. The sensitivity of these interactions to pressure offers a valuable experimental tool for investigating the energetics of the complexes.
Macgregor
Robert B
RB
Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada. rob.macgregor@utoronto.ca
D000818:Animals; D004268:DNA-Binding Proteins / Q000737:chemistry; D004798:Enzymes / Q000737:chemistry; D006801:Humans; D006874:Hydrostatic Pressure; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry
D004268:DNA-Binding Proteins; D004798:Enzymes; D009696:Nucleic Acids; D011506:Proteins