title: Neutrophils CD11b and fibroblasts PGE(2) are elevated in Alzheimer's disease.
abstract: To evaluate whether inflammation-like mechanisms present in the brain of Alzheimer's disease (AD) patients are reflected in the periphery, the expression of CD11b in peripheral blood neutrophils and the expression and activity of inflammatory markers in cultured skin fibroblasts were examined. We found significantly higher levels of CD11b in neutrophils from sporadic AD patients than in controls and this elevation was positively correlated with disease severity and progression rate of mental decline. Cultured skin fibroblasts from familial (FAD) and sporadic AD patients and from controls were immunopositive for both isoforms of cyclooxygenase with no differences between groups. In unstimulated culture, the production of prostaglandin-E2 in the medium was significantly higher in fibroblasts from sporadic AD and FAD patients than in controls, and this elevation was reverted by the addition of 25 microM of ibuprofen. Our findings provide further evidence of the presence of inflammatory and immuno-related markers in the periphery of AD patients and support those studies indicating the beneficial effects of anti-inflammatory therapy in AD.
authors: Department of Pharmacology, University of Florence, Viale Pieraccini, 6, 50139, Italy.
authors: Carla
authors: C
authors: Scali
authors: Costanza
authors: C
authors: Prosperi
authors: Laura
authors: L
authors: Bracco
authors: Carolina
authors: C
authors: Piccini
authors: Roberto
authors: R
authors: Baronti
authors: Andrea
authors: A
authors: Ginestroni
authors: Sandro
authors: S
authors: Sorbi
authors: Giancarlo
authors: G
authors: Pepeu
authors: Fiorella
authors: F
authors: Casamenti
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000097:blood / Q000378:metabolism* / Q000523:psychology; D002478:Cells, Cultured; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000097:blood*; D008297:Male; D008565:Membrane Proteins; D009483:Neuropsychological Tests; D009504:Neutrophils / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D016177:Macrophage-1 Antigen; D008565:Membrane Proteins; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone