title: Carp, a cardiac ankyrin-repeated protein, and its new homologue, Arpp, are differentially expressed in heart, skeletal muscle, and rhabdomyosarcomas.
abstract: Arpp, a protein containing an ankyrin repeat domain, PEST sequence, and proline-rich region, is a novel ankyrin-repeated protein highly homologous to Carp, which is proposed to be the putative genetic marker for cardiac hypertrophy. In this study, we comparatively analyzed expression of Arpp and Carp protein in skeletal and cardiac muscles and rhabdomyosarcomas (RMSs). In adult skeletal muscle, Arpp was preferentially expressed in the nucleus and cytoplasm of type I fibers, whereas Carp was barely detectable in skeletal muscle. On the other hand, in adult cardiac muscle, interestingly, Arpp was expressed in ventricles mostly, whereas Carp was expressed throughout the atrium and ventricle. Furthermore, although Carp was identified in fetal heart at 11 developmental weeks, Arpp was very low or undetectable in these fetal hearts. These results suggest that Arpp and Carp are differentially expressed and function in both skeletal and cardiac muscle of fetus and adult. We found that Arpp expression was induced during the differentiation of C2C12 cells in vitro, suggesting that Arpp-expression may be associated with the differentiation stage during myogenesis. Both Arpp and Carp were found to be expressed in all of the RMS cases studied. Because the expression patterns of Arpp in RMS were different from those of muscle actin or desmin, Arpp may be detectable in RMS cases that do not express other existing RMS markers.
authors: Department of Molecular Biology, Tottori University, Yonago, Japan.
authors: Naoko
authors: N
authors: Ishiguro
authors: Takeshi
authors: T
authors: Baba
authors: Tsuyoshi
authors: T
authors: Ishida
authors: Kengo
authors: K
authors: Takeuchi
authors: Mitsuhiko
authors: M
authors: Osaki
authors: Nobuhito
authors: N
authors: Araki
authors: Eikichi
authors: E
authors: Okada
authors: Satsuki
authors: S
authors: Takahashi
authors: Masahiro
authors: M
authors: Saito
authors: Mari
authors: M
authors: Watanabe
authors: Chisato
authors: C
authors: Nakada
authors: Yoshiyuki
authors: Y
authors: Tsukamoto
authors: Kenzo
authors: K
authors: Sato
authors: Kinji
authors: K
authors: Ito
authors: Masashi
authors: M
authors: Fukayama
authors: Shigeo
authors: S
authors: Mori
authors: Hisao
authors: H
authors: Ito
authors: Masatsugu
authors: M
authors: Moriyama
mesh_terms: D000199:Actins / Q000032:analysis; D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002454:Cell Differentiation; D002460:Cell Line; D002648:Child; D003893:Desmin / Q000032:analysis; D005260:Female; D005318:Fetal Heart / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D007890:Leiomyosarcoma / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D018657:Muscle Fibers, Slow-Twitch / Q000737:chemistry; D009124:Muscle Proteins; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000166:cytology; D009130:Muscle, Smooth / Q000737:chemistry; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009211:Myoglobin / Q000032:analysis; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D012208:Rhabdomyosarcoma / Q000378:metabolism* / Q000473:pathology; D017386:Sequence Homology, Amino Acid
chemical_list: C491304:ANKRD1 protein, human; C432364:ANKRD2 protein, human; D000199:Actins; D000906:Antibodies; D003893:Desmin; D009124:Muscle Proteins; D009211:Myoglobin; D009687:Nuclear Proteins; D012097:Repressor Proteins