title: In situ visualization of caspase-1-like activity associated with promotion of hippocampal cell death.
abstract: To clarify the function of caspase-1-like proteases in neuronal cell death, it is important to be able to detect the activity in living organs by microscopic visualization. In the present study, we synthesized a novel fluorescent substrate sensitive to the caspase-1-like activity, which is easily introduced into cells constituting living organs by extracellular application. As a result, the substrate was shown to be useful in imaging the caspase-1-like activity in rat hippocampal slice cultures. After induction of cell death with glutamate, a significant increase in the activity was observed, especially in the pyramidal cells, suggesting the association of the activity with promotion of cell death.
authors: School of Life Science, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan.
authors: Wataru
authors: W
authors: Nishii
authors: Takuji
authors: T
authors: Shoda
authors: Nagisa
authors: N
authors: Matsumoto
authors: Takeshi
authors: T
authors: Nakamura
authors: Yoshihisa
authors: Y
authors: Kudo
authors: Kenji
authors: K
authors: Takahashi
mesh_terms: D000818:Animals; D020170:Caspase 1 / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D016923:Cell Death; D018698:Glutamic Acid / Q000633:toxicity; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000379:methods*; D009842:Oligopeptides / Q000737:chemistry*; D009924:Organ Culture Techniques; D017966:Pyramidal Cells / Q000201:enzymology; D051381:Rats; D012235:Rhodamines / Q000737:chemistry*
chemical_list: C454919:N(alpha)-5-tetramethylrhodaminyl-Tyr-Val-Ala-Asp-Ala-Cys(S-acrylodan); D009842:Oligopeptides; D012235:Rhodamines; D018698:Glutamic Acid; D020170:Caspase 1