Different diets and amino acid supplementation do not affect the voluntary consumption of ethanol by rats.
The effects of four different diets (control diet: 19.5% protein, 60.5% carbohydrate, 10% fat; diet I: 65% protein, 10% carbohydrate, 10% fat; diet II: 5% protein, 76% carbohydrate, 10% fat; diet III: 20% protein, 69% carbohydrate, 1% fat; diet IV: 69% protein, 15% carbohydrate, 1% fat) and supplementation with 3 amino acids (tryptophan: 150 mg/kg/d; arginine: 400 mg/kg/d; taurine: 380 mg/kg/d) on the voluntary consumption of ethanol were investigated in rats using the 2 bottle method. First, rats received the control diet and diets I, II, III and IV for 20 days with a choice of ethanol for the last 6 days only. Ethanol consumption was similar in all dietary groups. Second, rats received the control diet for 8 days followed by diets I, II and IV for another 8 days. Ethanol was offered throughout both periods. The switch to the special diets did not affect ethanol consumption. Third, rats received a control diet with arginine, tryptophan or taurine added to the drinking fluids for 16 days with a choice of ethanol for the last 5 days; thereafter supplementation stopped but the ethanol choice remained. No difference in the voluntary intake of ethanol was noted but ethanol consumption fell after cessation of arginine supplementation. In conclusion, diets differing greatly in their composition or supplementation with these 3 amino acids did not affect the voluntary choice of ethanol by rats in a significant manner.
Much
Martin
M
Department of Biochemistry and Molecular Pharmacology, Jefferson Medical College of Thomas Jefferson University, Philadelphia, PA 19107, USA.
Dadmarz
Mitra
M
Hofford
James M
JM
Vogel
Wolfgang H
WH
D000428:Alcohol Drinking; D000596:Amino Acids / Q000008:administration & dosage*; D000818:Animals; D004032:Diet; D019587:Dietary Supplements; D051381:Rats; D017207:Rats, Sprague-Dawley; D012646:Self Administration
D000596:Amino Acids