Analysis of the NF2 gene in oligodendrogliomas and ependymomas.
Allelic losses of chromosome 22 are commonly found in ependymomas and oligodendrogliomas, suggesting that at least one tumor suppressor gene on chromosome 22 must be inactivated during the multistep process of tumorigenesis in these glial tumors. The neurofibromatosis 2 gene (NF2) located at 22q12, is a candidate tumor suppressor gene potentially involved in the pathogenesis of gliomas. Because there have been only a few studies of the NF2 gene in glial tumors other than astrocytoma, we screened the entire 17 NF2 exons for mutations in a series of 47 nonastrocytic tumors, including 40 oligodendrogliomas and 7 ependymomas. Only one mutation was detected, a 59-base pair insertion in exon 3 from a spinal anaplastic ependymoma. These results concur with previous findings proposing preferential inactivation of the NF2 gene in a subgroup of ependymomas, and suggest that the NF2 gene is not the target of chromosome 22 aberrations in oligodendrogliomas.
Alonso
M Eva
ME
Laboratorio de Oncogenética Molecular, Dept. C. Experimental, Hospital Universitario La Paz, Paseo de la Castellana 261, 28046 Madrid, Spain.
Bello
M Josefa
MJ
Arjona
Dolores
D
Gonzalez-Gomez
Pilar
P
Lomas
Jesus
J
de Campos
Jose M
JM
Kusak
M Elena
ME
Isla
Alberto
A
Rey
Juan A
JA
D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D004806:Ependymoma / Q000235:genetics* / Q000473:pathology; D005091:Exons / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D016254:Mutagenesis, Insertional; D009154:Mutation; D025581:Neurofibromin 2 / Q000235:genetics*; D009837:Oligodendroglioma / Q000235:genetics* / Q000473:pathology; D018807:Polymorphism, Single-Stranded Conformational
D004273:DNA, Neoplasm; D025581:Neurofibromin 2