title: Apolipoprotein C polymorphism in sheep: allele frequencies and association with plasma lipid and lipoprotein levels.
abstract: The genetic polymorphism of apolipoprotein C in normal plasma of four European sheep breeds (Suffolk, Corriedale, Cheviot, and Finn) was first detected using one-dimensional polyacrylamide gel isoelectric focusing (pH 2.5-5.0) followed by immunoblotting with antihuman apolipoprotein CII antibody. Six phenotypes (1-1, 2-1, 2-2, 3-1, 3-2, and 3-3) were identified in the 4.3-4.8 pH range, consisting of the combination of three isoform groups. On the basis of family and population data, these phenotypes were controlled autosomally by three codominant alleles, designated APOC*1, APOC*2, and APOC*3, the first being the most common allele. The frequency distributions of these alleles were similar between the Suffolk and Corriedale sheep, and between the Cheviot and Finn sheep. The former breeds had a significantly lower APOC*2 frequency than the latter breeds (P < 0.001). The mean plasma total-, HDL- and LDL-cholesterol levels of type 3-1 animals were significantly higher compared to type 1-1 animals in the Suffolk sheep (P < or = 0.04). However these differences were not seen in the Corriedale sheep.
authors: Department of Legal Medicine, Showa University School of Medicine, Tokyo, Japan.
authors: Kenji
authors: K
authors: Tsunoda
authors: Shohei
authors: S
authors: Hamato
authors: Shoei
authors: S
authors: Kurosawa
authors: Ayako
authors: A
authors: Shirato
authors: Yaetsu
authors: Y
authors: Kurosawa
authors: Koichiro
authors: K
authors: Fujimaki
authors: Masahiro
authors: M
authors: Muto
authors: Keizo
authors: K
authors: Sato
mesh_terms: D000818:Animals; D001056:Apolipoproteins C / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003433:Crosses, Genetic; D005260:Female; D005787:Gene Frequency; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007525:Isoelectric Focusing; D008055:Lipids / Q000097:blood*; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008297:Male; D010641:Phenotype; D011110:Polymorphism, Genetic; D020033:Protein Isoforms / Q000235:genetics; D012756:Sheep / Q000097:blood / Q000235:genetics*
chemical_list: D001056:Apolipoproteins C; D002788:Cholesterol Esters; D008078:Cholesterol, LDL; C059714:HDL cholesteryl ester; D008055:Lipids; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D020033:Protein Isoforms; D002784:Cholesterol