title: Characterization of the 16S-23S rRNA intergenic spacer regions among strains of the Fusobacterium necrophorum cluster.
abstract: The 16S-23S rRNA intergenic spacer regions (ISRs) of Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme were characterized. Products of two sizes, about 360 bp (small) and 530 bp (large), were generated by PCR amplification from the 16S-23S rRNA ISR of all the strains tested. The large and small 16S-23S rRNA ISRs of F. necrophorum exhibited a level of sequence similarity of 93.9% to 99.7% and 94.2% to 98.6% homologies within the species, respectively. Only the large spacer regions in these bacteria contained one or two tRNA genes. F. necrophorum subsp. necrophorum contains the isoleucine and alanine tRNA gene, whereas F. necrophorum subsp. funduliforme contains the isoleucine tRNA gene.
authors: Department of Veterinary Microbiology, Faculty of Agriculture, Miyazaki University, Japan.
authors: Jinghua
authors: J
authors: Jin
authors: Delong
authors: D
authors: Xu
authors: Wonganun
authors: W
authors: Narongwanichgarn
authors: Yoshitaka
authors: Y
authors: Goto
authors: Takeshi
authors: T
authors: Haga
authors: Toshiharu
authors: T
authors: Shinjo
mesh_terms: D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics*; D005675:Fusobacterium necrophorum / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S