Functional analysis of HIV-specific cytotoxic T lymphocytes in antiviral-treated- and-naive patients: a preliminary report.
HIV-specific CTL functions were analyzed in HIV-infected individuals who did or did not receive antiretroviral therapy (ART). Results showed that gp 160 (env)-stimulated perforin- and granzyme-expressing CTL, as well as perforin and granzyme-specific mRNA, were reduced in treated patients whereas TNFalpha was increased in ART-treated compared to naive individuals. Reduction of perforin and granzyme-expressing cells was not secondary to impaired IFNgamma production. A defect of CTL is observed in ART-treated individuals; this defect is not dependent on impaired Th cell function. These results reinforce the need for immunomodulants to successfully approach therapy of HIV infection.
Trabattoni
D
D
Chair of Immunology, Disp Lita Vialba, Universiy of Milan, Italy.
Fossati
S
S
Biasin
M
M
Boasso
A
A
Rizzardini
G
G
Maseratti
R
R
Clerici
M
M
D019380:Anti-HIV Agents / Q000494:pharmacology / Q000627:therapeutic use*; D018414:CD8-Positive T-Lymphocytes / Q000201:enzymology / Q000276:immunology; D003594:Cytoplasmic Granules / Q000201:enzymology; D003602:Cytotoxicity, Immunologic; D018076:DNA, Complementary / Q000235:genetics; D004790:Enzyme Induction; D015686:Gene Products, env / Q000494:pharmacology; D015658:HIV Infections / Q000188:drug therapy* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000032:analysis; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger / Q000032:analysis; D012697:Serine Endopeptidases / Q000032:analysis; D013602:T-Lymphocytes, Cytotoxic / Q000201:enzymology / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics; D014766:Viremia / Q000188:drug therapy / Q000276:immunology
D019380:Anti-HIV Agents; D018076:DNA, Complementary; D015686:Gene Products, env; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D054353:Perforin; D007371:Interferon-gamma; D012697:Serine Endopeptidases