title: Expression of aquaporin-5 and its regulation in skeletal muscle cells.
abstract: The aquaporins constitute a family of homologous intrinsic membrane proteins that function as highly selective water channels and are highly expressed in tissues where rapid water movement across the cell membrane is required. Molecular mechanism of water transport through the plasma membrane of skeletal muscle is still not clear. This study was designed to identify aquaporin subtypes and their expression regulation in C2C12 cells, a mouse myoblastic cell line. RT-PCR, immunohistochemistry and Western blot analysis revealed that C2C12 cells express AQP5. AQP5 expression was increased by induction of C2C12 differentiation. Exposure of C2C12 cells to hypertonic solutions induced an increase in AQP5 expression and p38 kinase activation. However, a p38 kinase inhibitor failed to inhibit hyperosmolar induction of AQP5 expression in C2C12 cells. These data indicate that skeletal muscle cells express AQP5 protein and its expression is regulated by differentiation and hypertonic stress.
authors: Research Center for Molecular Medicine, Department of Physiology, College of Medicine, Pusan National University, Korea.
authors: Sook Mi
authors: SM
authors: Hwang
authors: Ryang Hwa
authors: RH
authors: Lee
authors: Jin Mi
authors: JM
authors: Song
authors: Sick
authors: S
authors: Yoon
authors: You Sun
authors: YS
authors: Kim
authors: Sang Joon
authors: SJ
authors: Lee
authors: Soo Kyung
authors: SK
authors: Kang
authors: Jin Sup
authors: JS
authors: Jung
mesh_terms: D000818:Animals; D051402:Aquaporin 5; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D006982:Hypertonic Solutions; D008565:Membrane Proteins; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D020033:Protein Isoforms; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: C496038:Aqp5 protein, mouse; D051402:Aquaporin 5; D020346:Aquaporins; D006982:Hypertonic Solutions; D008565:Membrane Proteins; D020033:Protein Isoforms; D020928:Mitogen-Activated Protein Kinases