title: The LEF1/beta -catenin complex activates movo1, a mouse homolog of Drosophila ovo required for epidermal appendage differentiation.
abstract: Drosophila ovo/svb (dovo) is required for epidermal cuticle/denticle differentiation and is genetically downstream of the wg signaling pathway. Similarly, a mouse homolog of dovo, movo1, is required for the proper formation of hair, a mammalian epidermal appendage. Here, we provide biochemical evidence that movo1 encodes a nuclear DNA binding protein (mOvo1a) that binds to DNA sequences similar to those that dOvo binds to, further supporting the notion that mOvo1a and dOvo are genetically and biochemically homologous proteins. Additionally, we show that the movo1 promoter is activated by the lymphoid enhancer factor 1 (LEF1)/beta-catenin complex, a transducer of wnt signaling. Collectively, our findings suggest that movo1 is a developmental target of wnt signaling during hair morphogenesis in mice, and that the wg/wnt-ovo link in epidermal appendage regulatory pathways has been conserved between mice and flies.
authors: Department of Biological Chemistry, University of California, Irvine, CA 92697, USA.
authors: Baoan
authors: B
authors: Li
authors: Douglas R
authors: DR
authors: Mackay
authors: Qian
authors: Q
authors: Dai
authors: Tony W H
authors: TW
authors: Li
authors: Mahalakshmi
authors: M
authors: Nair
authors: Magid
authors: M
authors: Fallahi
authors: Christopher P
authors: CP
authors: Schonbaum
authors: Judith
authors: J
authors: Fantes
authors: Anthony P
authors: AP
authors: Mahowald
authors: Marian L
authors: ML
authors: Waterman
authors: Elaine
authors: E
authors: Fuchs
authors: Xing
authors: X
authors: Dai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000502:physiology*; D003850:Deoxyribonuclease I / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000196:embryology; D015151:Immunoblotting; D007150:Immunohistochemistry; D051821:Lymphoid Enhancer-Binding Factor 1; D051379:Mice; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators; D014157:Transcription Factors / Q000378:metabolism* / Q000502:physiology*; D014162:Transfection; D051176:beta Catenin
chemical_list: C495267:CTNNB1 protein, mouse; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C497574:Lef1 protein, mouse; D051821:Lymphoid Enhancer-Binding Factor 1; C490318:Ovo1 protein, mouse; D011994:Recombinant Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D051176:beta Catenin; D003850:Deoxyribonuclease I