Induction of cytochrome P450 1A and 2B by alpha- and beta-ionone in Sprague Dawley rats.
Beta-lonone has been reported to induce the cytochrome P450 (P450) 281 in rats. In this study, the effects of beta-ionone and an isomer, alpha-ionone, on liver P450 1A and 2B expression in Sprague Dawley rats were investigated. Subcutaneous administration of alpha- and beta-ionone 72 and 48 hr prior to sacrificing the animals induced the liver microsomal P450 1A and 2B proteins. P450 2B1 induction was associated with the accumulation of its corresponding mRNA. Induction by beta-ionone was much higher than that by a-ionone in both the mRNA and protein levels. When the route of administration was compared, P450 2B was induced more strongly after oral administration compared to that after subcutaneous injection. A single oral dose of 100, 300 and 600 mg/kg of alpha- and beta-ionone for 24 h induced P450 2B1-selective pentoxyresorufin O-depentylase activity comparably in a dose-dependent manner. In addition, alpha- and beta-ionone induced the P450 1A and 2B proteins. These results suggest that alpha- and beta-ionone might be potent P450 281 inducers in rats, and that both ionones may be useful for examining the role of metabolic activation in chemical-induced toxicity where metabolic activation is required.
Jeong
Hye Gwang
HG
College of Pharmacy, Chosun University, Kwangju, Korea.
Chun
Young-Jin
YJ
Yun
Chul-Ho
CH
Moon
Chang-Kiu
CK
Lee
Hye-Sook
HS
Han
Sang Seop
SS
Lee
Eung-Seok
ES
Jeong
Tae Cheon
TC
D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis* / Q000235:genetics; D019362:Cytochrome P-450 CYP2B1 / Q000096:biosynthesis* / Q000235:genetics; D004790:Enzyme Induction / Q000187:drug effects; D008297:Male; D045792:Norisoprenoids; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013729:Terpenes / Q000494:pharmacology*
D045792:Norisoprenoids; D012333:RNA, Messenger; D013729:Terpenes; C008157:beta-ionone; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1; C011879:alpha-ionone