Dugbe nairovirus S segment: correction of published sequence and comparison of five isolates.
The sequence of the S (small) RNA segment of the ArD 44313 isolate of Dugbe nairovirus (DUG) has been redetermined, and a number of apparent errors in the previously reported sequence (V. K. Ward, A. C. Marriott, A. A. El-Ghorr, and P. A. Nuttall, 1990, Virology 175, 518-524) were revealed. Our results indicate that the S RNA is 1716 nucleotides (nt) in length and contains one large open reading frame spanning 1449 nt. This can encode a 483 amino acid polypeptide, M(r) 53.9 kDa, corresponding to the viral nucleocapsid protein N. The DUG N protein is thus similar in length to the N proteins of Hazara (HAZ) and Crimean-Congo haemorrhagic fever (CCHF) nairoviruses, which are 485 and 482 amino acids in length, respectively. S segment RNA sequences were also determined for DUG isolates IbAr 1792, IbH 11480, ArD 16095, and KT 281/75; only the KT 281/75 sequence differed markedly from that of ArD 44313. Phylogenetic trees were constructed for these nairovirus S segment sequences.
Bridgen
Anne
A
Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, Church Street, Glasgow, G11 5JR, Scotland. A.Bridgen@vir.gla.ac.uk
Dalrymple
David A
DA
Elliott
Richard M
RM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D004279:DNA, Viral; D051379:Mice; D008969:Molecular Sequence Data; D016855:Nairovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral; D012367:RNA, Viral