title: Epsins and Vps27p/Hrs contain ubiquitin-binding domains that function in receptor endocytosis.
abstract: Ubiquitin functions as a signal for sorting cargo at multiple steps of the endocytic pathway and controls the activity of trans-acting components of the endocytic machinery (reviewed in refs 1, and 2). By contrast to proteasome degradation, which generally requires a polyubiquitin chain that is at least four subunits long, internalization and sorting of endocytic cargo at the late endosome are mediated by mono-ubiquitination. Here, we demonstrate that ubiquitin-interacting motifs (UIMs) found in epsins and Vps27p (ref. 9) from Saccharomyces cerevisiae are required for ubiquitin binding and protein transport. Epsin UIMs are important for the internalization of receptors into vesicles at the plasma membrane. Vps27p UIMs are necessary to sort biosynthetic and endocytic cargo into vesicles that bud into the lumen of a late endosomal compartment, the multivesicular body. We propose that mono-ubiquitin regulates internalization and endosomal sorting by interacting with modular ubiquitin-binding domains in core components of the protein transport machinery. UIM domains are found in a broad spectrum of proteins, consistent with the idea that mono-ubiquitin can function as a regulatory signal to control diverse biological activities.
authors: Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL, 60208-3500, USA.
authors: Susan C
authors: SC
authors: Shih
authors: David J
authors: DJ
authors: Katzmann
authors: Joshua D
authors: JD
authors: Schnell
authors: Myra
authors: M
authors: Sutanto
authors: Scott D
authors: SD
authors: Emr
authors: Linda
authors: L
authors: Hicke
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D002352:Carrier Proteins / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology*; D056827:Endosomal Sorting Complexes Required for Transport; D017930:Genes, Reporter; D009479:Neuropeptides / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D022161:Transport Vesicles / Q000737:chemistry / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism*; D033921:Vesicular Transport Proteins
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D002352:Carrier Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D009479:Neuropeptides; D011956:Receptors, Cell Surface; D029701:Saccharomyces cerevisiae Proteins; D025801:Ubiquitin; C119627:VPS27 protein, S cerevisiae; D033921:Vesicular Transport Proteins; C114154:epsin