Cryopreserved chronic lymphocytic leukemia cells analyzed by multicolor fluorescence in situ hybridization after optimized mitogen stimulation.
We investigated the utility of multicolor in situ fluorescence hybridization (mFISH) on cryopreserved blood cells from 11 chronic lymphocytic leukemia (CLL) patients. The results demonstrate that an individually chosen optimized mitogen combination induces proliferation of neoplastic B-cells after cryopreservation. Abnormal cells were detected in eight samples by mFISH, and, in six samples, the abnormality could be verified by comparative genomic hybridization or interphase FISH. In addition to typical CLL abnormalities, such as del(11q) or +12, several balanced translocations and single-cell abnormalities were found. Thus, mFISH can reveal new prognostically relevant chromosome aberrations in CLL.
Karhu
Ritva
R
Laboratory of Cancer Genetics, University of Tampere and Tampere University Hospital, Tampere, Finland.
Vilpo
Leena
L
Isola
Jorma
J
Knuutila
Sakari
S
Vilpo
Juhani
J
D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002869:Chromosome Aberrations; D020223:Chromosome Painting; D015925:Cryopreservation; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008934:Mitogens / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D008934:Mitogens