Diversity of glutamate dehydrogenase in human brain.
Three forms of glutamate dehydrogenase (GDH, EC 1.4.1.3) are purified from human brain tissue. Two of them, named GDH I (consisting of 58+/-1-kDa subunit) and GDH II (consisting of 56+/-1 -kDa subunit), are readily solubilized and the third one, GDH III (consisting of 56+/-1-kDa subunit), is a membrane-associated (particulate bound) isoform. Kinetic constants were determined for GDH III. These GDH forms were found to differ in hydrophobicity as indicated by different affinity to Phenyl-Sepharose. All three GDH forms showed microheterogeneity on two-dimensional (2-D) gel electrophoresis. Specific polyclonal antibodies, which enable to determine the levels of immunoreactivities of all the GDH forms in human brain extracts by enzyme-chemiluminescent amplified (ECL)-Western immunoblotting, were obtained.
Burbaeva
Gulnur Sh
GSh
Laboratory of Neurochemistry, Mental Health Research Center RAMS, Moscow, Russia. burbaeva@rcmh.msk.ru
Turishcheva
Marina S
MS
Vorobyeva
Elena A
EA
Savushkina
Olga K
OK
Tereshkina
Elena B
EB
Boksha
Irina S
IS
D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000276:immunology; D002417:Cattle; D005969:Glutamate Dehydrogenase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera / Q000378:metabolism; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D013552:Swine
D007106:Immune Sera; D007527:Isoenzymes; D005969:Glutamate Dehydrogenase