Genotypic and phenotypic expression of vocal fold polyps and Reinke's edema: a preliminary study.
Although a great deal of research exists regarding lamina propria composition, no report exists that relates gene expression in benign laryngeal lesions to phenotypic markers. In this study, messenger RNA profiles for extracellular matrix proteins--procollagen I, collagenase, elastase, fibronectin, fibromodulin, decorin, hyaluronic acid synthase 2, and hyaluronidase--were completed on 5 polyps and 4 Reinke's edema specimens. These genotypic profiles were correlated to a videostroboscopic parameter of mucosal wave stiffness, which was used as a measurement of phenotypic expression. Polyps, characterized by stiffer mucosal waves, had higher levels of gene expression, whereas stiffer mucosal wave scores for Reinke's edema were associated with lower gene activity levels. This study supports the hypothesis that there is a relationship between genotypic expression found in polyps and Reinke's edema and phenotype as defined by a loss of or a decreased mucosal wave. The study also gives clues as to the proteins responsible for the phenotype.
Thibeault
Susan L
SL
Department of Surgery, University of Wisconsin-Madison, USA.
Gray
Steven D
SD
Li
Wenhua
W
Ford
Charles N
CN
Smith
Marshall E
ME
Davis
R Kim
RK
D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics; D017931:DNA Primers; D058575:Decorin; D005109:Extracellular Matrix / Q000235:genetics; D016326:Extracellular Matrix Proteins; D000071235:Fibromodulin; D005353:Fibronectins / Q000235:genetics; D015870:Gene Expression; D005838:Genotype; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000235:genetics; D007819:Laryngeal Edema / Q000235:genetics*; D007822:Laryngeal Neoplasms / Q000235:genetics*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011127:Polyps / Q000235:genetics*; D011509:Proteoglycans / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D013053:Spectrophotometry; D014827:Vocal Cords
D002352:Carrier Proteins; C546878:DCN protein, human; D017931:DNA Primers; D058575:Decorin; D016326:Extracellular Matrix Proteins; C000604330:FMOD protein, human; D005353:Fibronectins; D011509:Proteoglycans; D012333:RNA, Messenger; D000071235:Fibromodulin; D006821:Hyaluronoglucosaminidase