Identification of radical scavengers in sweet grass (Hierochloe odorata).
Extracts from aerial parts of sweet grass (Hierochloe odorata) were active DPPH free radical scavengers. The active compounds were detected in extract fractions using HPLC with on-line radical scavenging detection. After multistep fractionation of the extract, two new natural products possessing radical scavenging activity were isolated, and their structures were elucidated by NMR and MS. They were identified as 5,8-dihydroxybenzopyranone and 5-hydroxy-8-O-beta-D-glucopyranosyl-benzopyranone. Activities of the compounds isolated were tested by DPPH and ABTS free radical scavenging assays, and compared with the known natural antioxidant rosmarinic acid and Trolox.
Pukalskas
Audrius
A
Department of Food Technology, Kaunas University of Technology, Radvilenu pl. 19, LT-3028 Kaunas, Lithuania.
van Beek
Teris A
TA
Venskutonis
Rimantas P
RP
Linssen
Jozef P H
JP
van Veldhuizen
Albertus
A
de Groot
Aede
A
D000975:Antioxidants / Q000032:analysis; D015764:Bepridil / Q000031:analogs & derivatives*; D001713:Biphenyl Compounds; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000032:analysis*; D005609:Free Radicals; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010851:Picrates; D006109:Poaceae / Q000737:chemistry*
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D007202:Indicators and Reagents; D010851:Picrates; D015764:Bepridil; C004931:1,1-diphenyl-2-picrylhydrazyl