DNA microarray assessment of putative Borrelia burgdorferi lipoprotein genes.
A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. These data show that a DNA microarray can globally examine the genes encoding B. burgdorferi lipoproteins.
Liang
Fang Ting
FT
Section of Rheumatology, Department of Internal Medicine, School of Medicine, Yale University, New Haven, Connecticut 06520-8031, USA.
Nelson
F Kenneth
FK
Fikrig
Erol
E
D001426:Bacterial Proteins / Q000235:genetics*; D025065:Borrelia burgdorferi / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D008074:Lipoproteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008074:Lipoproteins