T type 1/type 2 subsets balance in B-cell chronic lymphocytic leukemia--the three-color flow cytometry analysis.
The impairments in immune cell functions as well as changes in cytokine network are the cause of malignant cell accumulation and secondary immune deficiencies in B-cell chronic lymphocytic leukemia (B-CLL). To broaden the knowledge of immune system in B-CLL we tried to evaluate the Th1/Th2 and Tc1/Tc2 balance in B-CLL patients in comparison with healthy individuals and changes of this pattern during disease progression. The three-color flow cytometry technique was used to analyze IL-4 and IFNgamma expression in peripheral blood CD3+CD4+ and CD3+CD8+ cells. The results indicate the dominance of T type 1 cells and T-cell-mediated immunity in B-CLL patients that is however shifted towards T type 2 during disease progression.
Podhorecka
Monika
M
Department of Clinical Immunology, University School of Medicine in Lublin, Jaczewskiego 8, 20-950, Lublin, Poland. mpodhor@asklepios.am.lukin.pl
Dmoszynska
Anna
A
Rolinski
Jacek
J
Wasik
Ewa
E
D015415:Biomarkers; D017252:CD3 Complex / Q000097:blood; D018791:CD4 Lymphocyte Count / Q000295:instrumentation / Q000379:methods; D016827:CD8 Antigens / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry; D018450:Disease Progression; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology*; D018417:Th1 Cells / Q000737:chemistry; D018418:Th2 Cells / Q000737:chemistry
D015415:Biomarkers; D017252:CD3 Complex; D016827:CD8 Antigens; D015847:Interleukin-4; D007371:Interferon-gamma