Effect of dietary supplementation with a mixture of Vitamins C and E on fertilization of tertiary butyl hydroperoxide-treated oocytes and parthenogenetic activation in the mouse.
The present study aims to analyze the effect of dietary supplementation with a mixture of Vitamins C and E on fertilization and later development of tertiary butyl hydroperoxide (tBH)-treated mouse oocytes and on parthenogenetic activation of freshly ovulated mouse oocytes. We fed hybrid mice a standard diet supplemented or not supplemented with Vitamins C and E from the first day of weaning until the age of 12 weeks. We noted no significant effect of diet on fertilization rate, percentage of total and hatching blastocysts, total number of cells, mitotic index and percentage of apoptotic nuclei at 120 h post-insemination of oocytes incubated for 15 min in the presence of 0, 1, 5 and 10 microM tBH. Furthermore, diet did not affect the percentage of activated oocytes after treatment with Ca2+ ionophore, acid Tyrode's solution or ethanol. The percentage of parthenogenetically activated oocytes that progressed to the pronuclear stage was significantly higher in the antioxidant group. Oocytes from antioxidant females exhibited a significantly lower mitogen-activated protein kinase (MAPK) activity than oocytes from control females. We detected no significant differences between groups in M-phase-promoting factor (MPF) activity. These results show that oral administration of antioxidants decreases MAPK activity and increases the probability of reaching the pronuclear stage after parthenogenetic activation.
Tarín
J J
JJ
Department of Animal Biology, University of Valencia, Burjasssot, Spain. tarinjj@uv.es
Pérez-Albalá
S
S
García-Pérez
M A
MA
Cano
A
A
D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage*; D046508:Culture Techniques; D019587:Dietary Supplements; D005314:Embryonic and Fetal Development; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D005307:Fertilization in Vitro; D008297:Male; D016200:Maturation-Promoting Factor / Q000032:analysis; D000090204:Mesothelin; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D020928:Mitogen-Activated Protein Kinases / Q000032:analysis; D009865:Oocytes / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D010312:Parthenogenesis; D014810:Vitamin E / Q000008:administration & dosage*; D014886:Weaning; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
C000715528:Msln protein, mouse; D014810:Vitamin E; D020122:tert-Butylhydroperoxide; D016200:Maturation-Promoting Factor; D020928:Mitogen-Activated Protein Kinases; D000090204:Mesothelin; D001205:Ascorbic Acid