title: Isolation of oligosaccharides from a partial-acid hydrolysate of pneumococcal type 3 polysaccharide for use in conjugate vaccines.
abstract: A series of well-defined oligosaccharide fragments of the capsular polysaccharide of Streptococcus pneumoniae type 3 has been generated. Partial-acid hydrolysis of the capsular polysaccharide, followed by fractionation of the oligosaccharide mixture by Sepharose Q ion-exchange chromatography yielded fragments containing one to seven [-->3)-beta-D-GlcpA-(1-->4)-beta-D-Glcp-(1-->] repeating units. The isolated fragments were analysed for purity by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using an IonPac AS11 column, and their structures were verified by 1H NMR spectroscopy and nano-electrospray mass spectrometry. The oligosaccharides can be used to produce neoglycoprotein vaccines with a defined carbohydrate part.
authors: Department of Bio-Organic Chemistry, Bijvoet Center, Utrecht University, PO Box 80.075, NL-3508 TB, The Netherlands.
authors: Dirk J
authors: DJ
authors: Lefeber
authors: Ricardo
authors: R
authors: Gutiérrez Gallego
authors: Christian H
authors: CH
authors: Grün
authors: Daniela
authors: D
authors: Proietti
authors: Sandro
authors: S
authors: D'Ascenzi
authors: Paolo
authors: P
authors: Costantino
authors: Johannis P
authors: JP
authors: Kamerling
authors: Johannes F G
authors: JF
authors: Vliegenthart
mesh_terms: D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000737:chemistry*; D018074:Vaccines, Conjugate / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D018074:Vaccines, Conjugate