Synthesis and biodistribution of 211At-labeled, biotinylated, and charge-modified poly-L-lysine: evaluation for use as an effector molecule in pretargeted intraperitoneal tumor therapy.
Poly-L-lysine (7, 21, and 204 kDa) has been evaluated as an effector carrier for use in pretargeted intraperitoneal tumor therapy. For the synthesis, the epsilon-amino groups on the poly-L-lysine were modified in three steps utilizing conjugate biotinylation with biotin amidocaproate N-hydroxysuccinimide ester (BANHS), conjugate radiolabeling with (211)At using the intermediate reagent N-succinimidyl 3-(trimethylstannyl)benzoate (m-MeATE), and charge modification using succinic anhydride, resulting in an increase in the molecular weight of approximately 80% of the final product. The labeling of the m-MeATE reagent and subsequent conjugation of the polymer were highly efficient with overall radiochemical yields in the range of 60-70%. The in vitro avidin binding ability of the modified polymer was almost complete (90-95%), as determined by binding to avidin beads using a convenient filter tube assay. Following intraperitoneal (ip) injection in athymic mice, the 13 kDa polymer product was cleared mainly via the kidneys with fast kinetics (biological half-live T(b) approximately 2 h) and with low whole-body retention. The clearance of the 38 kDa polymer was distributed between kidneys and liver, and the 363 kDa polymer was mainly sequestered by the liver with a T(b) of 8 h. Increased tissue uptake in the thyroid, lungs, stomach, and spleen following the distribution of the large effector molecules (38 and 363 kDa) suggests that degradation of the polymers by the liver may release some of the label as free astatine/astatide.
Lindegren
Sture
S
Department of Radiation Physics, Göteborg University, Sahlgrenska University Hospital, SE-413 45, Sweden. sture.lindegren@radfys.gu.se
Andersson
Håkan
H
Jacobsson
Lars
L
Bäck
Tom
T
Skarnemark
Gunnar
G
Karlsson
Börje
B
D000614:Aminocaproates / Q000737:chemistry; D000818:Animals; D001246:Astatine / Q000737:chemistry*; D001360:Avidin / Q000378:metabolism*; D001565:Benzoates / Q000737:chemistry; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D019921:Biotinylation; D004337:Drug Carriers; D005260:Female; D006207:Half-Life; D007274:Injections, Intraperitoneal; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011107:Polylysine / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics*; D011108:Polymers / Q000493:pharmacokinetics; D014018:Tissue Distribution; D014298:Trimethyltin Compounds / Q000737:chemistry
D000614:Aminocaproates; D001565:Benzoates; D004337:Drug Carriers; D011108:Polymers; D014298:Trimethyltin Compounds; C061345:N-succinimidyl 3-(trimethylstannyl)benzoate; D001360:Avidin; D011107:Polylysine; D001710:Biotin; C048592:biotinyl-6-aminocaproic acid N-hydroxysuccinimide ester; D001246:Astatine