An IS900-like sequence found in a Mycobacterium sp. other than Mycobacterium avium subsp. paratuberculosis.
The insertion sequence IS900 has been considered specific for Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) and has, therefore, been used as the target gene for diagnostic PCR of M. paratuberculosis. From a healthy dairy cow we have isolated and characterised a mycobacterium harbouring one copy of a sequence with 94% identity to IS900 at the nucleic acid level. The isolate was shown to be related to Mycobacterium cookii, as assessed by 16S rRNA sequencing. Strong amplifications were obtained with several PCR primers described for detection of IS900. This finding shows the need of alternative PCR systems based on other genes than IS900 to confirm the presence of M. paratuberculosis.
Englund
Stina
S
Department of Bacteriology, National Veterinary Institute, SE-751 89 Uppsala, Sweden. stina.englund@sva.se
Bölske
Göran
G
Johansson
Karl Erik
KE
D000818:Animals; D002417:Cattle; D004251:DNA Transposable Elements / Q000235:genetics*; D009162:Mycobacterium avium / Q000145:classification / Q000235:genetics*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification / Q000235:genetics*; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004251:DNA Transposable Elements; D012336:RNA, Ribosomal, 16S