Alteration of the LRP1B gene region is associated with high grade of urothelial cancer.
We have delineated regions of interest at chromosome 2q21.2, 2q36.3, and 2q37.1 by deletion mapping of 114 urothelial cancers (UC). Altogether, 17%, 18%, and 63% of the G1, G2, and G3 tumors displayed loss of heterozygosity at chromosome 2q, respectively, The region at 2q21.2 was narrowed down to the LRP1B gene (NT_005129.6). Hemi- and homozygous deletion at the LRP1B gene region was seen in 31 of 114 UCs. Only 8% of the UCs with G1 and none with G2 tumors showed loss of heterozygosity at the LRP1B gene, whereas 49% of the G3 UCs had allelic loss at this region. RT-PCR analysis of the LRP1B gene showed the lack of expression of several exons in 2 of 9 cases analyzed. Our analysis suggests that the LRP1B gene is a candidate tumor suppressor gene in UCs.
Langbein
Sigrun
S
Laboratory of Molecular Oncology, Department of Urology, Institute of Human Genetics, Ruprecht-Karls-University, Heidelberg, Germany.
Szakacs
Orsolya
O
Wilhelm
Monica
M
Sukosd
Farkas
F
Weber
Susanne
S
Jauch
Anna
A
Lopez Beltran
Antonio
A
Alken
Peter
P
Kälble
Tilman
T
Kovacs
Gyula
G
D002277:Carcinoma / Q000145:classification / Q000235:genetics* / Q000473:pathology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002889:Chromosomes, Human, Pair 2; D044842:Cullin Proteins; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D009693:Nucleic Acid Hybridization; D011973:Receptors, LDL / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000145:classification / Q000235:genetics* / Q000473:pathology; D019459:Urothelium / Q000473:pathology
C503645:CUL3 protein, human; D018797:Cell Cycle Proteins; D044842:Cullin Proteins; D004273:DNA, Neoplasm; C434476:LRP1B protein, human; D011973:Receptors, LDL