title: Effect of estrogen and 1alpha,25(OH)2- vitamin D3 on the activity and growth of human primary osteoblast-like cells in vitro.
abstract: OBJECTIVE
  To evaluate effects of 17beta-E(2) and 1alpha,25(OH)(2)-vitamin D(3) on human osteoblast-like (hOB) cells.
  
  
  METHODS
  Controlled, experimental study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  hOB cell cultures were prepared from the upper femur of postmenopausal patients undergoing bipolar endoprosthesis arthroplasty for a fractured femoral neck.
  
  
  METHODS
  hOB cells were subcultured with either 17beta-E(2) or 1alpha,25(OH)(2)-vitamin D(3), or both.
  
  
  METHODS
  Cell proliferation and activity of alkaline phosphatase, osteocalcin, and interleukin-6.
  
  
  RESULTS
  17beta-E(2) significantly reduced interleukin-6 and osteocalcin to 34% and 60% of control value but induced alkaline phosphatase and cell proliferation to 183% and 150% of control value. 1alpha,25(OH)(2)-vitamin D(3) significantly decreased cell proliferation to 88% of that of control group, but 1alpha,25(OH)(2)-vitamin D(3) plus 17beta-E(2) showed no difference from the control group. Alkaline phosphatase and osteocalcin were significantly increased by 1alpha,25(OH)(2)-vitamin D(3) alone or combined with 17beta-E(2), to 169% and 198% and to 144% and 144% of control value, respectively. 1alpha,25(OH)(2)-vitamin D(3), with or without 17beta-E(2), decreased interleukin-6 levels to 27% and 38% of control group, respectively.
  
  
  CONCLUSIONS
  17beta-E(2) and 1alpha,25(OH)(2)-vitamin D(3) have effects on osteoblasts. The prevention of osteoporosis by estrogen may be related not only to direct effects on osteoblastic activity and proliferation but also to indirect effects on osteoclasts by the decrease of interleukin-6 secretion.
authors: Department of Obstetrics and Gynecology, Keelung Chang Gung Memorial Hospital, Republic of, Keelung, Taiwan, Republic of China.
authors: Fang-Ping
authors: FP
authors: Chen
authors: Ning
authors: N
authors: Lee
authors: Kun-Chuang
authors: KC
authors: Wang
authors: Yung-Kui
authors: YK
authors: Soong
authors: Ko-En
authors: KE
authors: Huang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004338:Drug Combinations; D004958:Estradiol / Q000494:pharmacology*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D015675:Osteocalcin / Q000037:antagonists & inhibitors; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000093242:Interleukin-6 Inhibitors
chemical_list: D000469:Alkaline Phosphatase; D004338:Drug Combinations; D004958:Estradiol; D015675:Osteocalcin; D014807:Vitamin D; D000093242:Interleukin-6 Inhibitors; C116441:2-methyl-1,25-dihydroxyvitamin D3