Phosphatidylethanol in rat organs after ethanol exposure.
BACKGROUND
Phosphatidylethanol (PEth) is an abnormal phospholipid formed in mammalian cells that have been exposed to ethanol. It has been suggested that PEth mediates some of the damaging effects of ethanol on cells. This study was performed to investigate the level of PEth in organs of rats after in vivo alcohol exposure.


METHODS
Three exposure models were studied: (1) acute, intraperitoneal injection of ethanol (n = 3 x 3); (2) chronic, forced ethanol drinking (n = 6); and (3) chronic, free choice of ethanol (n = 20). PEth was analyzed by high-performance liquid chromatography after lipid extraction of the organs.


RESULTS
One acute injection gave detectable PEth levels in most organs analyzed, with maximal levels reached after 2 hr. The highest levels were reached in intestines, stomach, and lung. No PEth was detected in skeletal muscle, pancreas, or testis. The two exposure models for oral intake of ethanol also gave detectable PEth levels in most organs. The highest levels were reached in stomach, lung, and spleen. PEth was detected in muscle only in animals with heavy total alcohol intake.


CONCLUSIONS
PEth is formed in most organs of rats exposed to ethanol acutely or chronically. Variations in PEth level and rates of PEth formation and PEth degradation are organ specific.
Aradottir
Steina
S
Department of Medical Neurochemistry, Institute of Laboratory Medicine, Lund University, Sweden. steina.aradottir@neurokemi.lu.se
Lundqvist
Christofer
C
Alling
Christer
C
D000284:Administration, Oral; D000428:Alcohol Drinking / Q000378:metabolism; D000818:Animals; D002492:Central Nervous System Depressants / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D002755:Choice Behavior / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D020404:Glycerophospholipids / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution / Q000187:drug effects / Q000502:physiology
D002492:Central Nervous System Depressants; D020404:Glycerophospholipids; C051521:phosphatidylethanol; D000431:Ethanol