Pronuclear abnormalities and cytoskeletal organization during assisted fertilization in a patient with multifollicular ovarian response.
OBJECTIVE
To analyze the distribution of a tubulins and acetylated alpha tubulins and the chromatin configuration in abnormally fertilized zygotes from a patient with a multifollicular ovarian response after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).


METHODS
Immunofluorescence and phase contrast microscopy was performed in abnormally fertilized zygotes.


RESULTS
After phase contrast microscopy analysis, immunofluorescence staining was performed in 20 oocytes that developed > or = 3 pronuclei (PN) and karyomeres after IVF-ICSI. Around 80% of the abnormal zygotes from IVF were the consequence of monospermic fertilizations. Retention of the second polar body (PB) and the presumptive split of > or =1 PN within the cytoplasm were the main events present in most oocytes after IVF-ICSI.


CONCLUSIONS
Fluorescence labeling of selected sperm and oocyte components affords a unique view of abnormal fertilized zygotes. Surprisingly, anomalies detected after IVF-ICSI showed similar etiologies in this special group of zygotes.
Rawe
V Y
VY
Centro de Estudios en Ginecología y Reproducción, CEGyR, Viamonte, Buenos Aires, Argentina. vanerawe@hotmail.com
Kopelman
S
S
Nodar
F N
FN
Olmedo
S Brugo
SB
Chillik
C F
CF
D000107:Acetylation; D000328:Adult; D002843:Chromatin / Q000648:ultrastructure; D015925:Cryopreservation; D003599:Cytoskeleton / Q000737:chemistry / Q000648:ultrastructure*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008540:Meiosis; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D016471:Ovarian Hyperstimulation Syndrome / Q000209:etiology; D010062:Ovulation Induction / Q000009:adverse effects; D011247:Pregnancy; D011499:Protein Processing, Post-Translational; D020554:Sperm Injections, Intracytoplasmic; D013082:Sperm Tail / Q000737:chemistry / Q000648:ultrastructure; D013094:Spermatozoa; D014404:Tubulin / Q000032:analysis / Q000737:chemistry; D015053:Zygote / Q000737:chemistry / Q000473:pathology*
D002843:Chromatin; D014404:Tubulin