title: Combined cuff and suture technique for orthotopic whole intestinal transplantation in rats.
abstract: For the purpose of immunological study on small intestinal transplantation (SIT), rat SIT models using direct suture technique widely have been used, which requires at least several months of training for microsurgery. Alternatively, a simple cuff technique for SIT has been mainly used by us, which reduces warm-ischemic time and the training period, but the entire intestinal grafts usually obtain a limited blood supply. This report describes a modification of a combined cuff and suture technique for rat SIT to aid beginning microsurgical transplantation researchers. The advantages are 1) use of only arterial suturing, making it easier for beginners, with the cuff technique applied to the more difficult vein anastomosis; 2) achievement of better arterial inflow and graft survival than when the arterial cuff technique is used; and 3) doing only partial clamping of the aorta, which improves animal survival and success of the procedure. A very high successful rate in orthotopic whole SIT was achieved even by beginners.
authors: First Department of Surgery, Okayama University Medical School, Japan.
authors: Atsunori
authors: A
authors: Nakao
authors: Kazunori
authors: K
authors: Tahara
authors: Seiichiro
authors: S
authors: Inoue
authors: Kouichi
authors: K
authors: Mizuta
authors: Takayuki
authors: T
authors: Takeichi
authors: Hiroo
authors: H
authors: Uchida
authors: Noriaki
authors: N
authors: Tanaka
authors: Eiji
authors: E
authors: Kobayashi
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D004195:Disease Models, Animal; D006085:Graft Survival; D007420:Intestine, Large / Q000637:transplantation; D007421:Intestine, Small / Q000637:transplantation; D008297:Male; D008866:Microsurgery / Q000379:methods; D016377:Organ Transplantation / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew; D012680:Sensitivity and Specificity; D013536:Suture Techniques; D016042:Transplantation, Heterotopic