Symbiotic and non-symbiotic expression of cgMT1, a metallothionein-like gene from the actinorhizal tree Casuarina glauca.
A clone for a type 1 metallothionein (cgMT1) was isolated from a Casuarina glauca nodule cDNA library. The corresponding gene belongs to a small family and is highly expressed in roots and nitrogen-fixing nodules, whereas low expression was observed in aerial parts of the plant. The promoter region of cgMT1 was isolated and fused to the beta-glucuronidase (gus) gene. Transgenic Casuarinaceae plants showed that the cgMT1 promoter was most active in roots and in the oldest region of the shoot. In situ hybridization indicated that in nodules cgMT1 transcript is present in mature Frankia-infected cells and in the pericycle. Possible roles for cgMT1 in symbiotic and nonsymbiotic tissues are discussed.
Laplaze
Laurent
L
Department of Plant Sciences, University of Cambridge, UK.
Gherbi
Hassen
H
Duhoux
Emile
E
Pawlowski
Katharina
K
Auguy
Florence
F
Guermache
Fathia
F
Franche
Claudine
C
Bogusz
Didier
D
D024506:5' Flanking Region / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006651:Histocytochemistry; D017403:In Situ Hybridization; D019684:Magnoliopsida / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000235:genetics*; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013559:Symbiosis / Q000235:genetics; D014158:Transcription, Genetic
D018076:DNA, Complementary; D018744:DNA, Plant; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D008668:Metallothionein; D005966:Glucuronidase