PCR tools for the differential diagnosis of Taenia saginata and Taenia solium taeniasis/cysticercosis from different geographical locations.
The potential value of PCRs in the species-specific diagnosis of have been investigated, using samples of T. saginata and T. solium from different geographical areas. The PCRs examining inter-species differences were based on the sequence of the HDP2 DNA fragment, specific for T. saginata/T. solium, and the sequence of the rDNA internal transcribed spacer 1 and spacer 2 (ITS-1 and ITS-2). This PCR analysis of DNA isolates confirmed morphologic diagnosis and allowed the speciation of samples too small or fragmented for morphologic identification, with clear and consistent inter-species differences between T. saginata (twenty-two) and T. solium (three) geographical isolates. Possible intra-species genomic variability, within these species, was similarly studied through analysis of PCR amplification products (PCR-RFLP) and only encountered one exceptional T. saginata isolate from Kenya, which yielded a unique PCR-RFLP pattern, different from T. saginata DNA of Mexican (one sample) and Spanish (seven samples) origin.
González
Luis Miguel
LM
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain.
Montero
Estrella
E
Puente
Sabino
S
López-Velez
Rogelio
R
Hernández
Marisela
M
Sciutto
Edda
E
Harrison
Leslie J S
LJ
Parkhouse
R Michael E
RM
Gárate
Teresa
T
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002417:Cattle; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007630:Kenya; D008297:Male; D008800:Mexico; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013030:Spain; D013045:Species Specificity; D013552:Swine; D013621:Taenia / Q000737:chemistry / Q000235:genetics / Q000254:growth & development*; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology
D000907:Antibodies, Bacterial; D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer