Molecular diagnosis of Granulicatella elegans on the cardiac valve of a patient with culture-negative endocarditis.
We herein report one case of culture-negative infectious endocarditis (IE) where the organism, Granulicatella elegans, was identified by molecular analysis using broad-range PCR primers complementary to the 16S rRNA gene on the removed valve. The results and utility of this method are discussed.
Casalta
J P
JP
Unité des Rickettsies, CNRS UMR 6020, IFR 48, Université de la Méditerranée, Faculté de Médecine, 27 boulevard Jean Moulin, 13385 Marseille cedex 05, France.
Habib
G
G
La Scola
B
B
Drancourt
M
M
Caus
T
T
Raoult
D
D
D000328:Adult; D017931:DNA Primers; D004697:Endocarditis, Bacterial / Q000175:diagnosis*; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis*; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013289:Streptococcaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014261:Tricuspid Valve / Q000382:microbiology*
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S