[Molecular identification of sibling Anopheles species: example of the Anopheles minimus and Anopheles dirus complexes, major malarial vectors in Southeast Asia].
Effective control of malaria vectors requires precise identification of species. This is especially important within complexes of species that cannot be distinguished based on morphological features. Two methods based on polymerase chain reaction (PCR) analysis have been developed to identify 2 species in the Anopheles minimus complex and 5 species of the Minimums group as well as 4 species of the Anopheles dirus complex. Association of oligonucleotide couples in the form of multiplex PCR has allowed development of two simple, reliable PCR techniques adapted to each one of these species complexes that comprise the major vectors of malaria in Southeast Asia. Specifically designed to meet the needs of entomologist working in the field for reliable, cost-effective tests, these techniques will facilitate assessment of the geographical distribution of each vector. These data will help to better target vector control measures.
Manguin
S
S
Centre de Biologie et Gestion des Populations (CBGP), IRD-Montpellier, France. manguin@mpl.ird.fr
Mouchet
J
J
Coosemans
M
M
D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001210:Asia, Southeastern / Q000453:epidemiology; D002965:Classification; D003362:Cost-Benefit Analysis; D004247:DNA / Q000032:analysis; D004772:Entomology / Q000379:methods; D005828:Genetics, Population; D006801:Humans; D007303:Insect Vectors; D008288:Malaria / Q000453:epidemiology / Q000635:transmission*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*
D004247:DNA