title: Antibody reactivity of a standardized human serum protein solution against a spectrum of microbial pathogens and toxins: comparison with fresh frozen plasma.
abstract: In this study, we compared a standardized solution of human serum protein (HSP) and fresh frozen plasma (FFP) with regard to the antibody specificity against a number of microbial pathogens and some important pathogenicity factors of bacterial pathogens. Due to the clinical use of HSP and FFP for therapeutical plasma exchange, we have chosen a spectrum of microbial pathogens for serological analysis that is critical in clinical settings. With the enzyme-linked immunosorbent assay technique, we could show that HSP contains marked IgG antibody reactivity against antigens of Escherichia coli, Campylobacter jejuni, Enterobacter sakazakii, Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, S. epidermidis, Streptococcus pneumoniae, Enterococcus faecalis, Chlamydia pneumoniae, and Candida albicans. Although no IgM antibodies against the pathogens tested could be detected in HSP, moderate IgA reactivity was found against 4 of 12 microbial antigens. Immunoblot analysis demonstrated specific IgA and IgG responses against the endoproteinase Glu-C and the superantigens enterotoxin A and B of S. aureus, the IgA-protease of Neisseria gonorrhoeae, and Shiga toxin 2 of enterohemorrhagic E. coli. By using 3 different HSP batches in parallel, we could demonstrate antibody reactivity against important microbial pathogens and toxins. This antibody profile is essentially more homogeneous than that of 3 batches of FFP.
authors: Institut für Hygiene und Mikrobiologie der Universität Würzburg, Würzburg; and Biotest Pharma GmbH, Dreieich, Germany. hschmidt@hygiene.uni-wuerzburg.de
authors: Herbert
authors: H
authors: Schmidt
authors: Reinhard
authors: R
authors: Lissner
authors: Wilhelm
authors: W
authors: Struff
authors: Oliver
authors: O
authors: Thamm
authors: Helge
authors: H
authors: Karch
mesh_terms: D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D001798:Blood Proteins; D017848:Blood-Borne Pathogens; D005656:Fungal Proteins / Q000276:immunology; D006801:Humans; D010949:Plasma; D010951:Plasma Exchange; D012996:Solutions; D014118:Toxins, Biological / Q000276:immunology*; D014774:Virulence
chemical_list: D000942:Antigens, Bacterial; D001798:Blood Proteins; D005656:Fungal Proteins; D012996:Solutions; D014118:Toxins, Biological