title: Degradation pathways of trichloroethylene and 1,1,1-trichloroethane by Mycobacterium sp. TA27.
abstract: We analyzed the kinetics and metabolic pathways of trichloroethylene and 1,1,1-trichloroethane degradation by the ethane-utilizing Mycobacterium sp. TA27. The apparent Vmax and Km of trichloroethylene were 9.8 nmol min(-1) mg of cells(-1) and 61.9 microM, respectively. The apparent Vmax and Km of 1,1,1-trichloroethane were 0.11 nmol min(-1) mg of cells(-1) and 3.1 microM, respectively. 2,2,2-trichloroethanol, trichloroacetic acid, chloral, and dichloroacetic acid were detected as metabolites of trichloroethylene. 2,2,2-trichloroethanol, trichloroacetic acid, and dichloroacetic acid were also detected as metabolites of 1,1,1-trichloroethane. The amounts of 2,2,2-trichloroethanol, trichloroacetic acid, chloral, and dichloroacetic acid derived from the degradation of 3.60 micromol trichloroethylene were 0.16 micromol (4.4%), 0.11 micromol (3.1%), 0.02 micromol (0.6%), and 0.02 micromol (0.6%), respectively. The amounts of 2,2,2-trichloroethanol, trichloroacetic acid and dichloroacetic acid derived from the degradation of 1.73 micromol 1,1,1-trichloroethane were 1.48 micromol (85.5%), 0.22 micromol (12.7%), and 0.02 micromol (1.2%), respectively. More than 90% of theoretical total chloride was released in trichloroethylene degradation. Chloral and 2,2,2-trichloroethanol were transformed into each other, and were finally converted to trichloroacetic acid, and dichloroacetic acid. Trichloroacetic acid and dichloroacetic acid were not degraded by strain TA27.
authors: National Institute for Environmental Studies and CREST, Japan Science and Technology Corporation, Tsukuba, Ibaraki.
authors: Akiko
authors: A
authors: Hashimoto
authors: Kazuhiro
authors: K
authors: Iwasaki
authors: Naou
authors: N
authors: Nakasugi
authors: Mutsuyasu
authors: M
authors: Nakajima
authors: Osami
authors: O
authors: Yagi
mesh_terms: D002250:Carbon Radioisotopes / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D009161:Mycobacterium / Q000378:metabolism*; D010084:Oxidation-Reduction; D014240:Trichloroethanes / Q000378:metabolism*; D014241:Trichloroethylene / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D014240:Trichloroethanes; C024566:1,1,1-trichloroethane; D014241:Trichloroethylene