title: A new syringopeptin produced by bean strains of Pseudomonas syringae pv. syringae.
abstract: Two strains (B728a and Y37) of the phytopathogenic bacterium Pseudomonas syringae pv. syringae isolated from bean (Phaseolus vulgaris) plants were shown to produce in culture both syringomycin, a lipodepsinonapeptide secreted by the majority of the strains of the bacterium, and a new form of syringopeptin, SP(22)Phv. The structure of the latter metabolite was elucidated by the combined use of mass spectrometry (MS), nuclear magnetic resonance (NMR) spectroscopy and chemical procedures. Comparative phytotoxic and antimicrobial assays showed that SP(22)Phv did not differ substantially from the previously characterized syringopeptin 22 (SP(22)) as far as toxicity to plants was concerned, but was less active in inhibiting the growth of the test fungi Rhodotorula pilimanae and Geotrichum candidum and of the Gram-positive bacterium Bacillus megaterium.
authors: Dipartimento di Scienze Biochimiche A. Rossi-Fanelli, Università di Roma La Sapienza, P.le A. Moro, 5, 00185 Rome, Italy. Ingeborg.Grgurina@uniroma1.it
authors: Ingeborg
authors: I
authors: Grgurina
authors: Feliciana
authors: F
authors: Mariotti
authors: Vincenzo
authors: V
authors: Fogliano
authors: Monica
authors: M
authors: Gallo
authors: Andrea
authors: A
authors: Scaloni
authors: Nicola S
authors: NS
authors: Iacobellis
authors: Pietro
authors: P
authors: Lo Cantore
authors: Luisa
authors: L
authors: Mannina
authors: Valeria
authors: V
authors: van Axel Castelli
authors: Maria Luigia
authors: ML
authors: Greco
authors: Antonio
authors: A
authors: Graniti
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000382:microbiology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D011549:Pseudomonas / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity; D014026:Nicotiana / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001427:Bacterial Toxins; D010456:Peptides, Cyclic; C464465:syringopeptin SP(22)Phv