title: Sequence and functional similarities between pro-apoptotic Bid and plant lipid transfer proteins.
abstract: Pro-apoptotic proteins of the Bcl-2 family are known to act on mitochondria and facilitate the release of cytochrome c, but the biochemical mechanism of this action is unknown. Association with mitochondrial membranes is likely to be important in determining the capacity of releasing cytochrome c. The present work provides new evidence suggesting that some pro-apoptotic proteins like Bid have an intrinsic capacity of binding and exchanging membrane lipids. Detailed analysis indicates a significant sequence similarity between a subset of Bcl-2 family proteins including Bid and Nix and plant lipid transfer proteins. The similar structural signatures could be related to common interactions with membrane lipids. Indeed, isolated Bid shows a lipid transfer activity that is even higher than that of plant lipid transfer proteins. To investigate the possible relevance of these structure-function correlations to the apoptotic action of Bid, cell free assays were established with isolated mitochondria, recombinant Bid and a variety of exogenous lipids. Micromolar concentrations of lysolipids such as lysophosphatidylcholine were found to change the association of Bid with mitochondria and also stimulate the release of cytochrome c promoted by Bid. The changes in mitochondrial association and cytochrome c release were enhanced by the presence of liposomes of lipid composition similar to that of mitochondrial membranes. Thus, a mixture of liposomes, mitochondria and key lysolipids could reproduce the conditions enabling Bid to transfer lipids between donor and acceptor membranes, and also change its reversible association with mitochondria. Bid was also found to enhance the incorporation of a fluorescent lysolipid, but not of a related fatty acid, into mitochondria. On the basis of the results presented here, it is hypothesised that Bid action may depend upon its capacity of exchanging lipids and lysolipids with mitochondrial membranes. The hypothesis is discussed in relation to current models for the integrated action of pro-apoptotic proteins of the Bcl-2 family.
authors: Cancer Research Campaign Molecular Pharmacology Group, School of Biological Sciences, Stopford Building, University of Manchester, M13 9PT, Manchester, UK. mauro1it@hotmail.com
authors: Mauro
authors: M
authors: Degli Esposti
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D051023:BH3 Interacting Domain Death Agonist Protein; D001896:Boron Compounds; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D005456:Fluorescent Dyes; D006801:Humans; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008246:Lysophospholipids / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D051023:BH3 Interacting Domain Death Agonist Protein; C494814:BID protein, human; D001896:Boron Compounds; D002352:Carrier Proteins; D003574:Cytochrome c Group; D005456:Fluorescent Dyes; D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D010940:Plant Proteins; C030363:lipid transfer protein