Calcyclin is an early vasopressin-induced gene in the renal collecting duct. Role in the long term regulation of ion transport.
Long-term effects of arginine vasopressin (AVP) in the kidney involve the transcription of unidentified genes. By subtractive hybridization experiments performed on the RCCD(1) cortical collecting duct cell line, we identified calcyclin as an early AVP-induced gene (1 h). Calcyclin is a calcium-binding protein involved in the transduction of intracellular signals. In the kidney, calcyclin was localized at the mRNA level in the glomerulus, all along the collecting duct, and in the epithelium lining the papilla. In RCCD(1) cells and in m-IMCD(3) inner medullary collecting duct cells, calcyclin was evidenced in the cytoplasm. Calcyclin mRNA levels were progressively increased by AVP treatment in RCCD(1) (1.7-fold at 4 h) and m-IMCD(3) (2-fold at 7.5 h) cells. In RCCD(1) cells, calcyclin protein levels were increased by 4 h of AVP treatment. In vivo, treatment of genetically vasopressin-deficient Brattleboro rats with AVP for 4 days induced an increase in both calcyclin and aquaporin-2 mRNA expression. Finally, introduction of anti-calcyclin antibodies into RCCD(1) cells by permeabilizing the plasma membrane prevented the long-term (but not short-term) increase in short-circuit current induced by AVP. Taken together, these results suggest that calcyclin is an early vasopressin-induced gene that participates in the late phase of the hormone response in transepithelial ion transport.
Courtois-Coutry
Nathalie
N
INSERM U478, Institut Fédératif de Recherches 02, Faculté de Médecine Xavier Bichat, Université Paris 7, 16 rue Henri Huchard, France.
Le Moellic
Cathy
C
Boulkroun
Sheerazad
S
Fay
Michel
M
Cluzeaud
Françoise
F
Escoubet
Brigitte
B
Farman
Nicolette
N
Blot-Chabaud
Marcel
M
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D018797:Cell Cycle Proteins; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D007150:Immunohistochemistry; D017136:Ion Transport; D007685:Kidney Tubules, Collecting / Q000187:drug effects* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000235:genetics*
D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489132:S100a6 protein, rat; D001127:Arginine Vasopressin