title: Platelet-derived growth factor D: tumorigenicity in mice and dysregulated expression in human cancer.
abstract: Platelet-derived growth factor (PDGF) has been directly implicated in developmental and physiological processes, as well as in human cancer and other proliferative disorders. We have recently isolated and characterized a novel protease-activated member of the PDGF family, PDGF D. PDGF D has been shown to be proliferative for cells of mesenchymal origin, signaling through PDGF receptors. Comprehensive and systematic PDGF D transcript analysis revealed expression in many cell lines derived from ovarian, renal, and lung cancers, as well as from astrocytomas and medulloblastomas. beta PDGF receptor profiling further suggested autocrine signaling in several brain tumor cell lines. PDGF D transforming ability and tumor formation in SCID mice was further demonstrated. Exploiting a sensitive PDGF D sandwich ELISA using fully human monoclonal antibodies, PDGF D was detected at elevated levels in the sera of ovarian, renal, lung, and brain cancer patients. Immunohistochemical analysis confirmed PDGF D localization to ovarian and lung tumor tissues. Together, these data demonstrate that PDGF D plays a role in certain human cancers.
authors: CuraGen Corp., Branford, Connecticut 06405, USA. wlarochelle@curagen.com
authors: William J
authors: WJ
authors: LaRochelle
authors: Michael
authors: M
authors: Jeffers
authors: Jose R F
authors: JR
authors: Corvalan
authors: Xiao-Chi
authors: XC
authors: Jia
authors: Xiao
authors: X
authors: Feng
authors: Sandra
authors: S
authors: Vanegas
authors: Justin D
authors: JD
authors: Vickroy
authors: Xiao-Dong
authors: XD
authors: Yang
authors: Francine
authors: F
authors: Chen
authors: Gadi
authors: G
authors: Gazit
authors: Jane
authors: J
authors: Mayotte
authors: Jennifer
authors: J
authors: Macaluso
authors: Beth
authors: B
authors: Rittman
authors: Frank
authors: F
authors: Wu
authors: Mohan
authors: M
authors: Dhanabal
authors: John
authors: J
authors: Herrmann
authors: Henri S
authors: HS
authors: Lichenstein
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002471:Cell Transformation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D016513:Mice, SCID; D009369:Neoplasms / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D020797:Receptor, Platelet-Derived Growth Factor beta