Effect of baicalein on apoptosis of the human Hep G2 cell line was induced by mitochondrial dysfunction.
The effects of baicalein on the human hepatoblastoma G2 (Hep G2) cell line were investigated in this study. By an SRB viability assay, we demonstrated that baicalein reduced the viability in a dose- and time-dependent manner. The apoptotic features such as chromatin condensation and DNA fragmentation were observed in the baicalein-treated cells. During the process of apoptosis, we noticed a sequential dissipation of mitochondrial membrane potential (DeltaPsim) and an apparent redistribution of cytochrome c from the mitochondria to the cytosol in baicalein-treated cells. Furthermore, the mitochondrial Bcl-2 protein represented a dramatic change in response to baicalein treatment. Altogether, our data suggested that the effect of baicalein on apoptosis of the human Hep G2 cell line was induced by mitochondrial dysfunction and Bcl-2 regulation.
Chang
Wen-Huei
WH
Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
Chen
Ching-Hsein
CH
Gau
Rung-Jiun
RJ
Lin
Chun-Ching
CC
Tsai
Ching-Lin
CL
Tsai
Kelvin
K
Lu
Fung-Jou
FJ
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D002470:Cell Survival / Q000187:drug effects; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism; D003574:Cytochrome c Group / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D019686:Lamiaceae; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects / Q000378:metabolism; D014407:Tumor Cells, Cultured
D002843:Chromatin; D003574:Cytochrome c Group; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; C006680:baicalein; D004247:DNA