title: Luminol-type chemiluminescence derivatization reagents for liquid chromatography and capillary electrophoresis.
abstract: The present paper provides the principles for chemiluminescence of luminol-type compounds and their wide and powerful application to the detection system in liquid chromatography and capillary electrophoresis as derivatization reagents. The reagents can be classified into two types, chemiluminescence labeling and chemiluminogenic reagents. The former reagents are highly chemiluminescent themselves and used for tagging their intense chemiluminophores to analytes, whereas the latter are weakly chemiluminescent but generate intense chemiluminescence by reaction with analytes. The liquid chromatographic methods utilizing chemiluminescence derivatizing reactions with luminol-type reagents allow the analytes to be detected at pmol-sub-fmol levels. Furthermore, the chemiluminogenic reactions show high selectivity owing to their selective reaction against the analytes permitting facile and reproducible detection.
authors: Faculty of Pharmaceutical Sciences, Fukuoka University, Nanakuma, Johnan, Japan. masayama@fukuoka-u.ac.jp
authors: Masatoshi
authors: M
authors: Yamaguchi
authors: Hideyuki
authors: H
authors: Yoshida
authors: Hitoshi
authors: H
authors: Nohta
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007202:Indicators and Reagents / Q000737:chemistry*; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents; D008165:Luminol