Quinine and citric acid elicit distinctive Fos-like immunoreactivity in the rat nucleus of the solitary tract.
The present experiment investigated Fos-like immunoreactivity (FLI) in the nucleus of the solitary tract (NST) after intraoral infusions of 0.1 M citric acid, 0.3 M NaCl, and 0.3-30 mM quinine monohydrochloride (QHCl) in awake, behaving rats. Increases in QHCl concentration produced increases in the numbers of FLI-labeled neurons in the rostral part of the intermediate (i(r)) and rostral (r) NST, but the topographic distribution of FLI was consistent across QHCl concentrations and distinctive compared with effects of citric acid. Quinine elicited FLI concentrated in the medial third of the nucleus; acid elicited more broadly distributed FLI concentrated farther laterally. Surprisingly, in contrast to QHCl and citric acid, NaCl produced FLI that was indistinguishable from that produced by water. Although the functional significance of these patterns is unknown, citric acid and QHCl are nonpreferred stimuli but produced different oromotor behaviors. QHCl (30 mM) elicited approximately 3.2 times as many gapes as citric acid (0.1 M), and acid elicited more ingestive responses. Parallel differences in FLI expression suggest that different NST regions may have distinctive roles in triggering oromotor behaviors.
Travers
Susan P
SP
Section of Oral Biology, College of Dentistry, The Ohio State University, Columbus, Ohio 43218-2357, USA. travers3@osu.edu
D000284:Administration, Oral; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002452:Cell Count; D019343:Citric Acid / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D011803:Quinine / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000008:administration & dosage; D017552:Solitary Nucleus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013268:Stimulation, Chemical; D013649:Taste / Q000187:drug effects / Q000502:physiology; D014851:Wakefulness; D014867:Water / Q000008:administration & dosage
D016760:Proto-Oncogene Proteins c-fos; D014867:Water; D019343:Citric Acid; D012965:Sodium Chloride; D011803:Quinine