m-Bop, a repressor protein essential for cardiogenesis, interacts with skNAC, a heart- and muscle-specific transcription factor.
The m-Bop protein encoded by the mouse Bop gene is strongly expressed in heart and skeletal muscle, and recent studies with Bop knockout mice have demonstrated that m-Bop is essential for cardiogenesis in vivo and can act as a HDAC-dependent repressor in vitro. In the present studies, m-Bop was observed to interact with skNAC, a reported transcriptional activator specific to heart and skeletal muscle. The amino-terminal S region of the split S-ET domain of m-Bop as well as the MYND domain were required for interaction with skNAC in both the two-hybrid system and in coimmunoprecipitation experiments from cultured mammalian cells. As shown previously for interaction of the MYND domain-containing transcriptional corepressor, BS69, with several viral and cellular oncoproteins, a PXLXP motif in skNAC was required for interaction with m-Bop. Similar kinetics of induction and localization of m-Bop and skNAC during the induction of myogenesis in cultured C2C12 cells suggests a possible associated role for these proteins during this process.
Sims
Robert J
RJ
Section of Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, The University of Texas, Austin, Texas 78712, USA.
Weihe
Elizabeth K
EK
Zhu
Li
L
O'Malley
Sean
S
Harriss
June V
JV
Gottlieb
Paul D
PD
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins; D002460:Cell Line; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D006321:Heart / Q000254:growth & development*; D006801:Humans; D007700:Kinetics; D051379:Mice; D018345:Mice, Knockout; D018832:Molecular Chaperones; D009124:Muscle Proteins; D018482:Muscle, Skeletal / Q000254:growth & development; D011485:Protein Binding; D015534:Trans-Activators / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D020798:Two-Hybrid System Techniques
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D018832:Molecular Chaperones; D009124:Muscle Proteins; C484340:SMYD1 protein, human; C096485:Smyd1 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; C094763:ZMYND11 protein, human; C490622:Zmynd11 protein, mouse; C096099:nascent-polypeptide-associated complex