title: Dietary modulation of circulating leptin levels: site-specific changes in fat deposition and ob mRNA expression.
abstract: In order to study the effects of diet on fat distribution, circulating leptin levels and ob mRNA expression, diets of different macronutrient composition were fed to lean mice and gold thioglucose-obese mice. A high-fat diet and 2 high-carbohydrate diets, one containing mostly high-glycaemic-index starch and the other containing low-glycaemic-index starch were fed ad libitum for 10 weeks and were compared to standard laboratory chow. Weight gain was attenuated by feeding low-glycaemic-index starch in all mice and by feeding a high-fat diet in lean mice. Reduced adiposity was seen in lean mice fed low-glycaemic-index starch, whereas increased adiposity was seen in both lean and obese mice fed on the high-fat diet. Circulating leptin levels, when corrected for adiposity, were decreased in all mice fed either the high-fat diet or the low-GI diet. In epididymal fat pads, decreased ob mRNA expression was seen after both high-fat and high-glycaemic-index starch feeding. These results show that diet macronutrient composition contributes to the variability of circulating leptin levels by the combined effects of diet on fat distribution and on site-specific changes in ob mRNA expression.
authors: Human Nutrition Unit, Department of Biochemistry, University of Sydney, Sydney NSW 2006, Australia.
authors: C G
authors: CG
authors: Walker
authors: J M
authors: JM
authors: Bryson
authors: J L
authors: JL
authors: Phuyal
authors: I D
authors: ID
authors: Caterson
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001823:Body Composition; D001835:Body Weight; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004041:Dietary Fats / Q000008:administration & dosage*; D038321:Glycemic Index; D007328:Insulin / Q000378:metabolism; D020738:Leptin / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008820:Mice, Obese; D009765:Obesity / Q000097:blood / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004040:Dietary Carbohydrates; D004041:Dietary Fats; D007328:Insulin; D020738:Leptin; D012333:RNA, Messenger