Structure of the O-polysaccharide from the lipopolysaccharide from Vibrio cholerae O6.
The O-polysaccharide from Vibrio cholerae O6 was isolated from the LPS by mild-acid hydrolysis and has been investigated by sugar and methylation analysis and NMR spectroscopy. The polysaccharide was also depolymerized with aqueous hydrofluoric acid to give the repeating unit and multiples thereof. The O-polysaccharide had the following tetrasaccharide repeating unit. Two O-acetyl groups are present, one of them making the GlcNAc residue fully substituted and the steric crowding considerable at the branching residue.
Bergström
Niklas
N
Analytical Unit, Clinical Research Centre, Karolinska Institute, Huddinge University Hospital, Novum, SE-141 86, Sweden.
Nair
G Balakrish
GB
Weintraub
Andrej
A
Jansson
Per-Erik
PE
D002240:Carbohydrate Sequence; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D014734:Vibrio cholerae / Q000737:chemistry*
D008070:Lipopolysaccharides; D019081:O Antigens