Gas chromatography-mass spectrometry analysis of 4-O-methylpyridoxine (MPN) in the serum of patients with ginkgo seed poisoning.
The 4-O-methylpyridoxine (MPN) present in the seeds of the Ginkgo biloba (maidenhair tree) has anti-vitamin B6 actions, and ginkgo seed poisoning can induce convulsions. We developed a specific quantitative method using gas chromatography-mass spectrometry for the analysis of MPN in human serum. The trifluoroacyl (TFA) derivative of MPN was obtained by treating MPN with trifluoroacetic anhydride at 50 degrees C for 5 min and remained stable for 6 h. The calibration curve of standard MPN obtained in the selective ion mode using the base ion (m/z 343) was linear between 100 pg and 10 ng, and the detection limit was 50 pg. The full mass spectrum of 100 pg of the TFA derivative of MPN was obtained easily. MPN was extracted from the serum with the use of a C18 solid-phase extraction cartridge. The recovery rate of MPN added to the serum at a concentration of 0.1 microg/mL was 90.0%.
Fujisawa
Manami
M
Department of Analytical Chemistry, Niigata College of Pharmacy, Japan. manami@hosp.niigata.niigata.jp
Hori
Yasushi
Y
Nakajima
Mariko
M
Shimada
Kenji
K
Yoshikawa
Hideto
H
Wada
Keiji
K
D000931:Antidotes / Q000627:therapeutic use; D002849:Chromatography, Gas; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D020441:Ginkgo biloba / Q000506:poisoning*; D006801:Humans; D007223:Infant; D013058:Mass Spectrometry; D010939:Plant Poisoning / Q000188:drug therapy; D011732:Pyridoxal Phosphate / Q000627:therapeutic use; D011736:Pyridoxine / Q000031:analogs & derivatives* / Q000097:blood* / Q000302:isolation & purification; D012639:Seeds / Q000506:poisoning; D012640:Seizures / Q000139:chemically induced
D000931:Antidotes; C001737:4-methoxymethylpyridoxine; D011732:Pyridoxal Phosphate; D011736:Pyridoxine