Solid lipid nanoparticles (SLN) as ocular delivery system for tobramycin.
Aim of this study was to evaluate solid lipid nanoparticles (SLN) as carriers for topical ocular delivery of tobramycin (TOB). The SLN were in the colloidal size range (average diameter below 100 nm; polydispersity index below 0.2) and contained 2.5% TOB as ion-pair complex with hexadecyl phosphate. The preocular retention of SLN in rabbit eyes was tested using drug-free, fluorescent SLN (F-SLN): these were retained for longer times on the corneal surface and in the conjunctival sac when compared with an aqueous fluorescent solution. A suspension of TOB-loaded SLN (TOB-SLN) containing 0.3% w/v TOB was administered topically to rabbits, and the aqueous humour concentration of TOB was determined up to six hours. When compared with an equal dose of TOB administered by standard commercial eyedrops, TOB-SLN produced a significantly higher TOB bioavailability in the aqueous humour.
Cavalli
Roberta
R
Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, I-10125, Torino, Italy. roberta.cavalli@unito.it
Gasco
M Rosa
MR
Chetoni
Patrizia
P
Burgalassi
Susi
S
Saettone
M Fabrizio
MF
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics*; D001082:Aqueous Humor / Q000378:metabolism; D019540:Area Under Curve; D001682:Biological Availability; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005123:Eye / Q000378:metabolism*; D007202:Indicators and Reagents; D007509:Irritants; D008081:Liposomes; D008297:Male; D011817:Rabbits; D014031:Tobramycin / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics*
D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D007509:Irritants; D008081:Liposomes; D014031:Tobramycin