Secretory patterns of growth hormone in dogs: circannual, circadian, and ultradian rhythms.
The objective was to characterize the circannual, circadian, and ultradian secretory patterns of growth hormone (GH) in intact crossbred and purebred dogs. In all experiments, blood samples were collected with minimal stress by direct peripheral venipuncture and GH was measured in plasma by a homologous radioimmunoassay. For circannual studies, samples were collected monthly from 6 male dogs between 15:00 and 17:30 h over a 1-year time span. For circadian studies, blood samples were collected at 145-minute intervals from 09:00 to 06:45 h of the following day in 14 female dogs. In ultradian experiments, blood samples were collected at 15-minute intervals for 2.5 h (15:00 to 17:30 h) in 7 males and 7 females. Plasma GH in male dogs remained without change in summer, autumn, and winter but declined (P < 0.01) in spring (LSM +/- SEM; 6.9 +/- 0.5; 6.0 +/- 0.5; 6.3 +/- 0.5; 4.3 +/- 0.5 ng/mL, respectively). No plasma GH circadian rhythmicity was detected. Nor was any ultradian pattern evident in either males or females. No gender-related differences were observed in ultradian GH plasma profiles. It is concluded that, while basal GH levels show seasonal fluctuations in dogs, neither circadian nor ultradian GH secretory fluctuations were present in the dogs assessed.
Gobello
Cristina
C
Instituto de Teriogenología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina. cgobello@netverk.com.ar
Corrada
Yanina A
YA
Castex
Gervasio L
GL
de la Sota
Rodolfo L
RL
Goya
Rodolfo G
RG
D000818:Animals; D002940:Circadian Rhythm; D004285:Dogs / Q000097:blood / Q000502:physiology*; D005260:Female; D013006:Growth Hormone / Q000378:metabolism*; D008297:Male; D010507:Periodicity; D011863:Radioimmunoassay / Q000662:veterinary; D012621:Seasons
D013006:Growth Hormone