Changes in markers of oxidative stress and membrane properties in synaptosomes from rats exposed prenatally to toluene.
The present study was undertaken in order to investigate if toluene induced oxidative stress in brains from rats exposed prenatally to 1800 ppm toluene 6 hr/day at days 7-20 during the pregnancy. 35-42 days after birth the rats were killed and synaptosomal fractions were prepared for the experiments. Synaptosomes from rats exposed prenatally to toluene exhibited an increased level of oxidative stress when incubated with toluene in vitro compared to synaptosomes from unexposed offspring. Also the cell membrane was affected, as the calcium leakage was more increased from exposed synaptosomes than from unexposed. The membrane fluidity increased significantly when synaptosomes were incubated with toluene for 10 min. in vitro but the change in fluidity was identical in both groups of offspring. The results indicate that prenatal exposure to toluene induces long-lasting changes in oxidative status and membrane function.
Edelfors
Sven
S
Department of Pharmacology, Panum Institute, University of Copenhagen, Denmark. sven.edelfors@farmakol.ku.dk
Hass
Ulla
U
Hougaard
Karin S
KS
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects*; D005260:Female; D008431:Maternal-Fetal Exchange; D018384:Oxidative Stress / Q000187:drug effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000633:toxicity*; D013574:Synaptosomes / Q000187:drug effects*; D014050:Toluene / Q000008:administration & dosage / Q000633:toxicity*
D012997:Solvents; D014050:Toluene