Characterization of cDNA clones encoding ribonucleoprotein antigens expressed in Trypanosoma cruzi amastigotes.
Sera from patients with Chagas' disease were used to screen a Trypanosoma cruzi amastigote cDNA library. Characterization of 50 positive clones showed that 21 (42%) encode previously identified T. cruzi ribosomal and flagellar proteins, heat-shock proteins or proteins with repetitive motifs. Twenty-nine clones (58%) correspond to nine genes not previously described in T. cruzi. Three cDNAs, encoding novel repetitive antigens with homology to ribosomal proteins and to other RNA binding proteins, were further characterized. Patient humoral responses against the recombinant proteins encoded by these cDNAs were evaluated in anticipation that they may constitute potential new targets for serodiagnostic assays.
DaRocha
Wanderson D
WD
Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Bartholomeu
Daniella C
DC
Macêdo
Camila D S
CD
Horta
M Fátima
MF
Cunha-Neto
Edécio
E
Donelson
John E
JE
Teixeira
Santuza M R
SM
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D014355:Chagas Disease / Q000097:blood / Q000276:immunology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D017125:Genes, Protozoan; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D012261:Ribonucleoproteins / Q000276:immunology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000276:immunology*
D000953:Antigens, Protozoan; D018076:DNA, Complementary; D016054:DNA, Protozoan; D012333:RNA, Messenger; D012261:Ribonucleoproteins