Simple high-density lipoprotein cholesterol assay based on dry chemistry.
BACKGROUND
Currently, high-density lipoprotein cholesterol (HDL-C), a factor which prevents progression of arteriosclerosis, is measured using laboratory-based chemistry analyzers without a pretreatment step. Because HDL-C is measured with a pretreatment step in many point-of-care testing systems, a direct assay is needed.


METHODS
A dry-chemistry-based assay using surfactants has recently been developed in parallel with the development of a dedicated reagent. A simple analyzer that accepts whole blood samples was also developed.


RESULTS
The assay demonstrated excellent precision, dilution linearity and intermethod comparison. In an interference test, assay values tended to be lower in the presence of high concentrations of hemoglobin, conjugated or unconjugated bilirubin. Neither ascorbic acid up to 20 mg/dl, nor formazin turbidity up to 2100, had an effect on the assay.


CONCLUSIONS
This dry-chemistry assay using only surfactants for specificity in the direct HDL-C method was judged useful for point-of-care instrumentation in terms of equipment compactness, operational simplicity and rapid responsiveness.
Yamada
Tetsuaki
T
Department of Clinical Hematology and Clinical Diagnostics, Graduate School of Medicine, Osaka City University Medical School, 1-4-3 Asahimachi, Abeno, Osaka 545-8585, Japan.
Nishino
Susumu
S
Takubo
Takayuki
T
Hino
Masayuki
M
Kitagawa
Seiichi
S
Tatsumi
Noriyuki
N
D001774:Blood Chemical Analysis / Q000379:methods*; D008076:Cholesterol, HDL / Q000097:blood*; D006801:Humans; D016015:Logistic Models; D019095:Point-of-Care Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D013501:Surface-Active Agents; D013997:Time Factors
D008076:Cholesterol, HDL; D013501:Surface-Active Agents