title: A comparison of local and peripheral parasite-specific antibody production in different strains of mice infected with Trichuris muris.
abstract: The serum parasite-specific antibody responses of different mouse strains infected with Trichuris muris reflect the nature of the T-helper response mounted by the host, in that resistant Th2-responding strains, such as BALB/K, produce immunoglobulin (Ig)G1 and susceptible predominantly Th1-responding strains, such as AKR, produce IgG2a and IgG1. However, the kinetics of antibody production in the sera, as determined by enzyme-linked immunosorbent assay, do not reflect infection status in that resistant strains can expel their worm burdens before antibodies are detectable in the sera. Here, we show that parasite-specific antibody production by in vitro lipopolysaccharide-stimulated mesenteric lymph node cells (MLN) not only correlate with serum antibody isotypes, but also follow expulsion kinetics. Additionally, the antibody levels seen locally match changes in absolute B220+ cell numbers in the MLN (determined by flow cytometry) and changes in MLN parasite-specific plasma cells in the MLN (determined by ELISPOT). These results show that B cell responses are tightly regulated locally in both resistant and susceptible strains of mice infected with T. muris.
authors: School of Biological Sciences, University of Manchester, Manchester, UK.
authors: Nathan M
authors: NM
authors: Blackwell
authors: Kathryn J
authors: KJ
authors: Else
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D007070:Immunoglobulin A / Q000032:analysis* / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008297:Male; D008643:Mesentery / Q000033:anatomy & histology / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D013045:Species Specificity; D014257:Trichuriasis / Q000276:immunology* / Q000469:parasitology; D014258:Trichuris / Q000276:immunology* / Q000469:parasitology
chemical_list: D000909:Antibodies, Helminth; D007070:Immunoglobulin A; D007074:Immunoglobulin G