title: A simple method for enrichment of uninucleate conidia of Aspergillus oryzae.
abstract: Aspergillus oryzae produces multinucleate conidia, which makes the obtaining of homokaryons labor-intensive. Analysis of conidia by flow cytometry clarified the relationship that conidia of lower nuclear number were smaller in size. Based on this, we have developed a simple way to enrich uninucleate conidia with a membrane filter. Our results also suggest that the method is useful for elimination of heterokaryons.
authors: Noda Institute for Scientific Research, Noda-shi, Chiba, Japan. shara@mail.kikkoman.co.jp
authors: Seiichi
authors: S
authors: Hara
authors: Ryohei F
authors: RF
authors: Tsuji
authors: Osamu
authors: O
authors: Hatamoto
authors: Tsutomu
authors: T
authors: Masuda
mesh_terms: D001236:Aspergillus oryzae / Q000254:growth & development* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D005434:Flow Cytometry; D008863:Microspheres; D012542:Scattering, Radiation; D014462:Ultrafiltration