Preventive effects of Impatiens balsamina on the hen egg-white lysozyme (HEL)-induced decrease in blood flow.
Monitoring the blood flow of unanesthesized mice was found to be a reliable and effective method for studying their anaphylactic responses, in addition to the known method of monitoring blood pressure. Hen egg-white lysozyme (HEL)-specific anaphylaxis in mice was estimated by monitoring the decrease in blood flow with a Doppler blood flow meter. This method is convenient for searching for both anaphylaxis and anti-anaphylactic substances from natural products. Using this system, we estimated the anti-anaphylactic effects of the 35% ethanol extract (IB) of petals of Impatiens balsamina L., as well as those of anti-allergic agents currently used. Kaempferol 3-rutinoside and lawsone from IB significantly inhibited the decrease of blood flow. We also found that platelet-activating factor (PAF) and serotonin participate in decreasing the blood flow, but histamine does not.
Ishiguro
Kyoko
K
School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Hyogo, Japan. ishiguro@mwu.mukogawa-u.ac.jp
Ohira
Yukiko
Y
Oku
Hisae
H
D000707:Anaphylaxis / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology*; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects / Q000502:physiology; D029069:Impatiens / Q000737:chemistry*; D008297:Male; D051379:Mice; D009113:Muramidase / Q000009:adverse effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D018514:Plant Structures; D012039:Regional Blood Flow / Q000187:drug effects / Q000502:physiology; D013623:Tail / Q000098:blood supply* / Q000187:drug effects*
D010936:Plant Extracts; C421864:hen egg lysozyme; D009113:Muramidase