Evaluation of an immunofluorescent-antibody test using monoclonal antibodies directed against Enterocytozoon bieneusi and Encephalitozoon intestinalis for diagnosis of intestinal microsporidiosis in Bamako (Mali).
A 2-month study was carried out in Mali to evaluate an immunofluorescent-antibody test (IFAT) using monoclonal probes specific for Enterocytozoon bieneusi or Encephalitozoon intestinalis. Sixty-one human immunodeficiency virus (HIV)-seropositive adult patients and 71 immunocompetent children were enrolled. Microsporidia were detected in stools from 8 of 61 patients (13.1%) seropositive for HIV. A single species, E. bieneusi, was identified. All the children were negative for microsporidia. The sensitivity and specificity of IFAT were 100% compared with those of PCR, which was used as the "gold standard." Moreover, species identification by IFAT was more rapid and less expensive than that by PCR. These results show the suitability of IFAT for detection of microsporidia in developing countries.
Alfa Cisse
O
O
Unité INSERM 511, Laboratoire de Parasitologie-Mycologie, Centre Hospitalier-Universitaire de la Pitié-Salpêtrière, 47 Boulevard de l'Hôpital, 75013 Paris, France.
Ouattara
A
A
Thellier
M
M
Accoceberry
I
I
Biligui
S
S
Minta
D
D
Doumbo
O
O
Desportes-Livage
I
I
Thera
M A
MA
Danis
M
M
Datry
A
A
D000328:Adult; D000818:Animals; D002648:Child; D016819:Encephalitozoon / Q000302:isolation & purification*; D016890:Encephalitozoonosis / Q000175:diagnosis*; D021862:Enterocytozoon / Q000302:isolation & purification*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006679:HIV Seropositivity / Q000469:parasitology; D006801:Humans; D007121:Immunocompetence; D008302:Mali; D016881:Microsporidiosis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity