Crystal structure of murine sCEACAM1a[1,4]: a coronavirus receptor in the CEA family.
CEACAM1 is a member of the carcinoembryonic antigen (CEA) family. Isoforms of murine CEACAM1 serve as receptors for mouse hepatitis virus (MHV), a murine coronavirus. Here we report the crystal structure of soluble murine sCEACAM1a[1,4], which is composed of two Ig-like domains and has MHV neutralizing activity. Its N-terminal domain has a uniquely folded CC' loop that encompasses key virus-binding residues. This is the first atomic structure of any member of the CEA family, and provides a prototypic architecture for functional exploration of CEA family members. We discuss the structural basis of virus receptor activities of murine CEACAM1 proteins, binding of Neisseria to human CEACAM1, and other homophilic and heterophilic interactions of CEA family members.
Tan
Kemin
K
Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
Zelus
Bruce D
BD
Meijers
Rob
R
Liu
Jin-huan
JH
Bergelson
Jeffrey M
JM
Duke
Norma
N
Zhang
Rongguang
R
Joachimiak
Andrzej
A
Holmes
Kathryn V
KV
Wang
Jia-huai
JH
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D002272:Carcinoembryonic Antigen / Q000737:chemistry; D015815:Cell Adhesion Molecules; D017124:Conserved Sequence; D017934:Coronavirus / Q000737:chemistry / Q000502:physiology; D018360:Crystallography, X-Ray; D006023:Glycoproteins / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011485:Protein Binding / Q000502:physiology; D020033:Protein Isoforms / Q000737:chemistry; D017434:Protein Structure, Tertiary; D000085064:Receptors, Coronavirus; D011991:Receptors, Virus / Q000737:chemistry*; D016415:Sequence Alignment
D015703:Antigens, CD; C069847:CD66 antigens; D002272:Carcinoembryonic Antigen; C486183:Ceacam1 protein, mouse; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D020033:Protein Isoforms; D000085064:Receptors, Coronavirus; D011991:Receptors, Virus