Incorporation of horseradish peroxidase in a Kieselguhr membrane and the application to a mediator-free hydrogen peroxide sensor.
Horseradish peroxidase was incorporated in a kieselguhr membrane. The electron-transfer process of the enzyme was examined by cyclic voltammetry. It was observed that the electron-transfer reactivity of horseradish peroxidase was greatly enhanced, and that direct electrochemistry was accordingly feasible. Using the merits of the direct electron-transfer reactivity of horseradish peroxidase and its specific enzymatic catalysis towards hydrogen peroxide, an unmediated hydrogen peroxide biosensor was constructed. The calibration plot of this hydrogen peroxide sensor was linear in the range of 2.0 x 10(-6) mol/L - 6.5 x 10(-4) mol/L. The relative standard deviation was 4.1% for 6 successive determinations at a concentration of 1.0 x 10(-4) mol/L. The detection limit was 1.0 x 10(-6) mol/L.
Fan
C
C
Department of Biochemistry and National Laboratory of Pharmaceutical Biotechnology, Nanjing University, P R China.
Wang
H
H
Zhu
D
D
Wagner
G
G
Li
G
G
D015374:Biosensing Techniques; D002384:Catalysis; D007692:Diatomaceous Earth; D004563:Electrochemistry; D004800:Enzymes, Immobilized; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D010084:Oxidation-Reduction
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D007692:Diatomaceous Earth; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase