Comparison of three DNA marker systems for assessing genetic diversity in Asian arowana (Scleropages formosus).
Three DNA marker systems -- random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and microsatellites -- were used to estimate the genetic diversity in Asian arowana (Scleropages formosus) by genotyping fish individuals from three different sources. Parallel application of the three DNA marker systems allowed us to compare their utility for the analysis of genetic diversity. Microsatellites displayed the highest expected heterozygosity, whereas the values obtained by RAPD and AFLP were much lower. Multiplex ratio and marker index were higher for AFLP than for RAPD or microsatellites. Weak correlation was detected between genetic similarity estimated from data obtained with the three DNA marker systems: estimates from RAPD and AFLP data turned out to be higher than those from microsatellites. On the other hand genetic similarity was higher in the red variety than in the green one, especially when tested with microsatellites. Based on the genetic distance matrices calculated from microsatellite analysis, all red individuals were clustered into one group, whereas only a subset of them was clustered when either RAPD or AFLP was used. This indicated that the microsatellite system detected population subdivision more efficiently than either RAPD or AFLP.
Yue
Genhua
G
Laboratory of Fish Reproduction and Biotechnology, Institute of Molecular Agrobiology, National University of Singapore, Singapore.
Li
Yang
Y
Chen
Fan
F
Cho
Serena
S
Lim
Lian Chuan
LC
Orban
Laszlo
L
D000818:Animals; D016000:Cluster Analysis; D017931:DNA Primers; D005399:Fishes / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D005819:Genetic Markers