Environmental induction models for the investigation of activity: changes in glutathione peroxidase, a crucial factor of the antioxidant defence.
Glutathione peroxidase enzyme superfamily plays significant role in the elimination of reactive oxygen free radicals in the animals. Many characteristics of these proteins have been revealed already, but their regulation is still not known. Several data suggest that some environmental factors have certain regulatory effect, while others propose strict genetic regulation. In this report we present four different environmental induction models in which New Zealand white rabbits were used as experimental animals. In three models, free radical load of different origin, lipidperoxide load, application of a glutathione depletor or a prooxidant agent, was introduced. Beside these negative models a positive model was also constructed in which additive selenium was supplied. Glutathione peroxidase activity was measured in blood serum, erythrocyte haemolysate and liver. Reduced glutathione, and malondialdehyde concentration in the liver were also determined. According to the results, the established models are capable for analysing the enzyme activity x environmental interactions.
Erdélyi
M
M
Szent István University, Department of Nutrition, Gödöllo, Hungary. erdelyi@fau.gau.hu
Mézes
M
M
Virág
G
G
D000818:Animals; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005978:Glutathione / Q000172:deficiency*; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism*; D008054:Lipid Peroxides / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D012643:Selenium / Q000494:pharmacology*
D008054:Lipid Peroxides; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D005978:Glutathione; D012643:Selenium