title: LC/MS studies on characterization and determination of N,N'-ethylenebisdithiocarbamate fungicides in environmental water samples.
abstract: Liquid chromatography/mass spectrometry (LC/MS) has been applied to analyze N,N'-ethylenebisdithiocarbamate fungicides (EBDCs: manzeb, maneb and zineb) in environmental water samples. The EBDCs that are zinc and/or manganese salts are transformed into readily water-soluble sodium salts by adding an alkaline EDTA solution. The N,N'-ethylenebisdithiocarbamate anion is extracted into chloroform-hexane (3:1) as an ion pair with tetrabutylammonium, and then S-alkylated with methyl iodide. The extraction and derivatization are carried out at room temperature. The derivatized ethylenebisdithiocarbamic acid dimethyl ester is introduced into an LC/MS equipped with a negative ion electrospray ionization interface. Identification of the compound is performed with the specific quasi-molecular ion, and the quantitative analyses are carried out using the peak areas. The average recoveries and coefficients of variation of EBDCs at sub-ppb level are 79.1% and 29.3% (n = 6), respectively. The limit of detection based on standard deviation of 0.043 microg/L for manzeb is achieved.
authors: Kitakyushu City Institute of Environmental Sciences, Tobata, Japan.
authors: Yoshifumi
authors: Y
authors: Hanada
authors: Teizi
authors: T
authors: Tanizaki
authors: Minoru
authors: M
authors: Koga
authors: Hiroaki
authors: H
authors: Shiraishi
authors: Mitsuyuki
authors: M
authors: Soma
mesh_terms: D002138:Calibration; D005618:Fresh Water / Q000032:analysis; D005659:Fungicides, Industrial / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008344:Maneb / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014881:Water Supply / Q000032:analysis*; D015038:Zineb / Q000032:analysis*
chemical_list: D005659:Fungicides, Industrial; D008344:Maneb; C013099:mancozeb; D015038:Zineb