title: Production and characterization of a pig line transgenic for human membrane cofactor protein.
abstract: A pig line transgenic for human membrane cofactor protein (hMCP) has been established. Offspring from the founder were produced by crossing the founder with pigs heterozygous for the human decay accelerating factor (hDAF) transgene. As a result, pigs transgenic for both hMCP and hDAF have been produced. Ribonuclease protection assay (RPA) indicated that hMCP was expressed in all the tissues analysed. In addition, immunohistochemical results indicated a high level of expression of hMCP on neural tissues and islets where hDAF was absent or weakly expressed. C3 fragment deposition and cytotoxicity assays indicated that hMCP expression alone on pig endothelial cells and peripheral blood lymphocytes (PBLs) provided protection against human complement mediated damage. However, we did not find that porcine endothelial cells expressing both hDAF and hMCP were better protected than those expressing hDAF alone. The expression of hMCP on tissues where hDAF is not expressed could provide these tissues with protection against human complement mediated lysis.
authors: Imutran Ltd (A Novartis Pharma AG Co.), Cambridge, UK. chunyanzhou@bjmu.edu
authors: Chun-Yan
authors: CY
authors: Zhou
authors: Elizabeth
authors: E
authors: McInnes
authors: Nicola
authors: N
authors: Parsons
authors: Gillian
authors: G
authors: Langford
authors: Richard
authors: R
authors: Lancaster
authors: Andrew
authors: A
authors: Richards
authors: Gilda
authors: G
authors: Pino-Chavez
authors: Gabriela
authors: G
authors: Dos Santos Cruz
authors: Laura
authors: L
authors: Copeman
authors: Christine
authors: C
authors: Carrington
authors: Simon
authors: S
authors: Thompson
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics* / Q000276:immunology*; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000235:genetics*; D018958:CD55 Antigens / Q000235:genetics; D003176:Complement C3 / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D004730:Endothelium, Vascular / Q000276:immunology; D015870:Gene Expression; D005818:Genetic Engineering; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control; D006801:Humans; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000235:genetics*; D013552:Swine / Q000235:genetics* / Q000276:immunology*; D014183:Transplantation, Heterologous
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C497857:CD46 protein, human; D018958:CD55 Antigens; D003176:Complement C3; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins