[Determination of medullasin levels for the diagnosis of multiple sclerosis].
Medullasin levels in granulocytes of patients with neurological diseases and healthy volunteers were determined by the enzyme immunoassay using mouse monoclonal antibodies against human medullasin and o-phenylenediamine-H2O2 as the detection system of the enzyme activity. One hundred twenty-one out of 159 patients with multiple sclerosis (76.1%) showed positive results (above means of normals + 2SD) in this test, while only 16.9% (24/142) of patients with non-inflammatory neurological diseases had positive results. This enzyme immunoassay method for medullasin is considered to be an useful paraclinical test for the diagnosis of multiple sclerosis.
Aoki
Yosuke
Y
Department of Food and Health Science, Faculty of Human Life Science, Jissen Women's University, Hino 191-8510.
Saida
Takahiko
T
Nakano
Imaharu
I
Saito
Toyokazu
T
Ikeguchi
Kunihiko
K
Urabe
Takao
T
Nishiguchi
Etsuko
E
Suzuki
Hideaki
H
Takahashi
Kiyoshi
K
Mizuno
Yoshikuni
Y
D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000175:diagnosis*; D012697:Serine Endopeptidases / Q000097:blood*
D012697:Serine Endopeptidases; C034869:medullasin