title: A tomato enzyme catalyzing the phosphorylation of 3,4-dihydroxy-2-butanone.
abstract: A riboflavin biosynthesis ribB mutant of Escherichia coli deficient of 3,4-dihydroxy-2-butanone 4-phosphate synthase was complemented with a cDNA library from Lycopersicon esculentum. The complementing gene was isolated and expressed in E. coli. The resulting protein was shown to specify a 62 kDa protein which phosphorylates dihydroxyacetone, both enantiomers of 3,4-dihydroxy-2-butanone, and several other aldoses and ketoses. Sequence analysis revealed homology to dihydroacetone kinases (dak) genes from plants, animals, fungi and some eubacteria. Genes with similarity to the 5' part of the dak gene from tomato were found in many other eubacteria. The physiological role of the dak gene is still incompletely known.
authors: Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstr. 4, D-85747 Garching, Germany.
authors: Stefan
authors: S
authors: Herz
authors: Klaus
authors: K
authors: Kis
authors: Adelbert
authors: A
authors: Bacher
authors: Felix
authors: F
authors: Rohdich
mesh_terms: D000595:Amino Acid Sequence; D002074:Butanones / Q000378:metabolism*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005470:Fluorometry; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D012256:Riboflavin / Q000096:biosynthesis; D013379:Substrate Specificity
chemical_list: C460540:3,4-dihydroxy-2-butanone; D002074:Butanones; D007527:Isoenzymes; D017853:Phosphotransferases (Alcohol Group Acceptor); C023132:glycerone kinase; D012256:Riboflavin