Virosome-mediated delivery of protein antigens to dendritic cells.
Virosomes are reconstituted viral membranes in which protein can be encapsulated. Fusion-active virosomes, fusion-inactive virosomes and liposomes were used to study the conditions needed for delivery of encapsulated protein antigen ovalbumin (OVA) to dendritic cells (DCs) for MHC class I and II presentation. Fusion-active virosomes, but not fusion-inactive virosomes, were able to deliver OVA to DCs for MHC class I presentation at picomolar OVA concentrations. Fusion activity of virosomes was not required for MHC class II presentation of antigen. Therefore, virosomes are an efficient system for delivery of protein antigens for stimulation of both helper and CTL responses.
Bungener
Laura
L
Department of Medical Microbiology, Molecular Virology Section, University of Groningen, Groningen, The Netherlands.
Serre
Karine
K
Bijl
Liesbeth
L
Leserman
Lee
L
Wilschut
Jan
J
Daemen
Toos
T
Machy
Patrick
P
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D015703:Antigens, CD / Q000096:biosynthesis; D018122:B7-1 Antigen / Q000096:biosynthesis; D051940:B7-2 Antigen; D015415:Biomarkers; D019013:CD40 Antigens / Q000096:biosynthesis; D003593:Cytoplasm; D003713:Dendritic Cells / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D008081:Liposomes; D008561:Membrane Fusion; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010047:Ovalbumin / Q000276:immunology; D017452:Receptors, IgG / Q000276:immunology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D022701:Virosomes
D000941:Antigens; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D015415:Biomarkers; D019013:CD40 Antigens; C497965:Cd86 protein, mouse; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D008081:Liposomes; D008562:Membrane Glycoproteins; D017452:Receptors, IgG; D022701:Virosomes; D018799:Intercellular Adhesion Molecule-1; D010047:Ovalbumin