title: Toxicities of two freshwater puffers in Taiwan.
abstract: Forty-two and twelve specimens of puffers Tetraodon ocellatus and T. nigroviridis were, respectively, collected from the aquaria in Taiwan and determined for toxicity by using tetrodotoxin bioassay. It was found that T. ocellatus contained moderate amounts of toxin in skin and viscera, and the highest toxicity scores were 432 MU/g skin and 212 MU/g viscera. The specimens of T. nigroviridus contained weak amounts of toxin in skin, and the highest toxicity score was 124 MU/g. The toxin was partially purified from the toxic specimens of each species by ultrafiltration using a YM-1 membrane, followed by chromatography on Bio-Gel P-2 column. Analyses by electrophoresis, thin layer chromatography, and high performance liquid chromatography showed that the toxin from both species was composed of tetrodotoxin and anhydrotetrodotoxin.
authors: Department of Food Health, Deh Yu College of Nursing and Management, Keelung, Taiwan, ROC.
authors: Lin
authors: L
authors: Shin-Jung
authors: Chien-Fu
authors: CF
authors: Liao
authors: Osamu
authors: O
authors: Arakawa
authors: Tamao
authors: T
authors: Noguchi
authors: Deng-Fwu
authors: DF
authors: Hwang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004588:Electrophoresis, Cellulose Acetate; D005517:Foodborne Diseases / Q000517:prevention & control; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012621:Seasons; D012867:Skin / Q000737:chemistry; D013045:Species Specificity; D013624:Taiwan; D023721:Tetraodontiformes / Q000378:metabolism*; D013779:Tetrodotoxin / Q000302:isolation & purification* / Q000633:toxicity*; D023382:Toxicity Tests, Acute; D014781:Viscera / Q000737:chemistry
chemical_list: D013779:Tetrodotoxin