Glycerol-3-phosphate-induced catabolite repression in Escherichia coli.
The formation of glycerol-3-phosphate (G3P) in cells growing on TB causes catabolite repression, as shown by the reduction in malT expression. For this repression to occur, the general proteins of the phosphoenolpyruvate-dependent phosphotransferase system (PTS), in particular EIIA(Glc), as well as the adenylate cyclase and the cyclic AMP-catabolite activator protein system, have to be present. We followed the level of EIIA(Glc) phosphorylation after the addition of glycerol or G3P. In contrast to glucose, which causes a dramatic shift to the dephosphorylated form, glycerol or G3P only slightly increased the amount of dephosphorylated EIIA(Glc). Isopropyl-beta-D-thiogalactopyranoside-induced overexpression of EIIA(Glc) did not prevent repression by G3P, excluding the possibility that G3P-mediated catabolite repression is due to the formation of unphosphorylated EIIA(Glc). A mutant carrying a C-terminally truncated adenylate cyclase was no longer subject to G3P-mediated repression. We conclude that the stimulation of adenylate cyclase by phosphorylated EIIA(Glc) is controlled by G3P and other phosphorylated sugars such as D-glucose-6-phosphate and is the basis for catabolite repression by non-PTS compounds. Further metabolism of these compounds is not necessary for repression. Two-dimensional polyacrylamide gel electrophoresis was used to obtain an overview of proteins that are subject to catabolite repression by glycerol. Some of the prominently repressed proteins were identified by peptide mass fingerprinting. Among these were periplasmic binding proteins (glutamine and oligopeptide binding protein, for example), enzymes of the tricarboxylic acid cycle, aldehyde dehydrogenase, Dps (a stress-induced DNA binding protein), and D-tagatose-1,6-bisphosphate aldolase.
Eppler
Tanja
T
Department of Biology, University of Konstanz, 78457 Konstanz, Germany.
Postma
Pieter
P
Schütz
Alexandra
A
Völker
Uwe
U
Boos
Winfried
W
D000262:Adenylyl Cyclases / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D004268:DNA-Binding Proteins; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins; D005990:Glycerol / Q000494:pharmacology; D005994:Glycerophosphates / Q000494:pharmacology; D007763:Lac Operon; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000032:analysis / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D014157:Transcription Factors / Q000378:metabolism
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D005994:Glycerophosphates; C058976:MalT protein, E coli; D014157:Transcription Factors; C032242:crr protein, E coli; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D000262:Adenylyl Cyclases; D005990:Glycerol