title: Osmolarity hypersensitivity of hog1 deleted mutants is suppressed by mutation in KSS1 in budding yeast Saccharomyces cerevisiae.
abstract: An osmosensing mechanism of Saccharomyces cerevisiae involves a mitogen-activated protein kinase (MAPK) cascade (HOG pathway). This study aimed to investigate the response of the yeast to osmotic stress. A mutant strain, in which the HOG1 gene was disrupted by TRP1, was constructed. A spontaneous mutant, named YJY45, which suppresses the osmosensitive growth phenotype of the hog1 deletion mutant, was selected and showed a secondary phenotype of temperature sensitivity on YPD containing 0.5 M NaCl at 37 degrees C. Our data indicate that the spontaneous mutation in YJY45 mutant was mapped in KSS1, which is one of the MAPK family. The mutation in KSS1 suppresses the osmolarity-hypersensitive phenotype of the hog1 deletion mutation and restores GPD1 induction.
authors: Department of Molecular Biology, Pusan National University, 609-735, South Korea.
authors: Soo-Jung
authors: SJ
authors: Lee
authors: Shi-Young
authors: SY
authors: Park
authors: Jong-Gil
authors: JG
authors: Na
authors: Yung-Jin
authors: YJ
authors: Kim
mesh_terms: D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics; D020935:MAP Kinase Signaling System / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000502:physiology*; D016254:Mutagenesis, Insertional; D009994:Osmolar Concentration; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D017384:Sequence Deletion; D015398:Signal Transduction / Q000235:genetics; D013489:Suppression, Genetic; D014157:Transcription Factors / Q000502:physiology
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005993:Glycerolphosphate Dehydrogenase; C075580:FUS3 protein, S cerevisiae; C088589:HOG1 protein, S cerevisiae; C541984:KSS1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases