Impedimetric immunosensor using avidin-biotin for antibody immobilization.
The potentialities of an electrodeposited biotinylated polypyrrole film as an immobilisation matrix for the fabrication of impedimetric immunosensors are described. Biotinylated antibody (anti-human IgG), used as a model system, was attached to free biotin groups on the electrogenerated polypyrrole film using avidin as a coupling reagent. This immobilization method allows to obtain a highly reproducible and stable device. The resulting immunosensor has a linear dynamic range of 10-80 ng ml(-1) of antigen and a detection limit of 10 pg ml(-1). Furthermore, this immunosensor exhibited minor loss in response after two regeneration steps.
Ouerghi
O
O
Ingénierie et Fonctionnalisation des Surfaces, UMR CNRS 5621, Ecole Centrale de Lyon, BP 163, F-69131 Ecully Cédex, France.
Touhami
A
A
Jaffrezic-Renault
N
N
Martelet
C
C
Ouada
H Ben
HB
Cosnier
S
S
D001360:Avidin / Q000737:chemistry*; D015374:Biosensing Techniques; D001710:Biotin / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*
D007074:Immunoglobulin G; D001360:Avidin; D001710:Biotin