Simplified high-performance liquid chromatographic method for determination of risperidone and 9-hydroxyrisperidone in plasma after overdose.
For toxicological purposes, a HPLC assay was developed for the simultaneous determination of risperidone and 9-hydroxyrisperidone in human plasma. After a single-step liquid-liquid extraction, both compounds were separated on a C(18) column and measured at 280 nm. A good inter-assay accuracy (<116%) was achieved with inter-assay precision less than 12%. Quantification limits were 10 ng/ml. This rapid method (run time <5 min) is currently used for poison management.
Titier
K
K
Department of Pharmacology and Toxicology, EA 525, University Victor Segalen, Place Amélie Raba-Léon, 33076 Bordeaux, Cedex, France.
Déridet
E
E
Cardone
E
E
Abouelfath
A
A
Moore
N
N
D014150:Antipsychotic Agents / Q000009:adverse effects / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D062787:Drug Overdose / Q000097:blood*; D006801:Humans; D007555:Isoxazoles / Q000097:blood*; D000068882:Paliperidone Palmitate; D011743:Pyrimidines / Q000097:blood*; D015203:Reproducibility of Results; D018967:Risperidone / Q000009:adverse effects / Q000097:blood*; D012680:Sensitivity and Specificity
D014150:Antipsychotic Agents; D007555:Isoxazoles; D011743:Pyrimidines; D018967:Risperidone; D000068882:Paliperidone Palmitate