Complex formation of cytochrome C with a calixarene carboxylic acid derivative: a novel solubilization method for biomolecules in organic media.
A calixarene carboxylic acid derivative has been found to form a complex with the cationic protein cytochrome c. The solubilized cytochrome c was stable and showed peroxidase activity in chloroform. The calix[6]arene and the calix[8]arene achieved quantitative extraction of the protein. The calix[6]arene, whose cavity is well-fitted to a protonated amino group, exhibited a selectivity to lysine-rich proteins due to the recognition of the epsilon-amino groups in lysine residues on the surface of the protein. This is the first report showing protein extraction by calixarenes. The solubilized cytochrome c could catalyze an oxidative reaction in organic solvents. This host compound functions as a novel solubilization tool for biomolecules and a separation tool for lysine-rich proteins.
Oshima
Tatsuya
T
Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan.
Goto
Masahiro
M
Furusaki
Shintaro
S
D000818:Animals; D047250:Calixarenes; D002264:Carboxylic Acids; D002725:Chloroform; D003574:Cytochrome c Group / Q000737:chemistry* / Q000378:metabolism; D006736:Horses; D007700:Kinetics; D046911:Macromolecular Substances; D009930:Organic Chemicals; D010084:Oxidation-Reduction; D011485:Protein Binding; D012995:Solubility; D012997:Solvents
D002264:Carboxylic Acids; D003574:Cytochrome c Group; D046911:Macromolecular Substances; D009930:Organic Chemicals; D012997:Solvents; D047250:Calixarenes; D002725:Chloroform