Cloning and characterization of Igsf9 in mouse and human: a new member of the immunoglobulin superfamily expressed in the developing nervous system.
We describe the cloning and characterization of a novel member of the immunoglobulin superfamily, Igsf9. The predicted protein structure of IGSF9 closely matches that of the neural cell-adhesion molecule (NCAM) subfamily, consisting of an extracellular region containing five immunoglobulin domains and two fibronectin type III (FnIII) repeats, a transmembrane region, and a cytoplasmic tail. We have also characterized the orthologous human IGSF9 gene at 1q22-q23, revealing a highly conserved sequence and genomic organization. Expression of Igsf9 was detected by RT-PCR in mouse embryonic RNA from embryonic day (E) 7.5 to E16.5, while whole-mount in situ hybridization at E10.5 shows intense expression within the dorsal root ganglia, trigeminal ganglia, and olfactory epithelium, and less intense expression in the neuroepithelium, retina, and hindgut. In the human, transcription was detected in a wide variety of fetal tissues at both 8 and 14 weeks. Protein homology of IGSF9 is most similar to the Drosophila melanogaster Turtle protein that functions in coordinated motor output in complex behaviors.
Doudney
Kit
K
Department of Maternal and Fetal Medicine, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College, Hammersmith Campus, London, W12 0NN, UK.
Murdoch
Jennifer N
JN
Braybrook
Claire
C
Paternotte
Caroline
C
Bentley
Louise
L
Copp
Andrew J
AJ
Stanier
Philip
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009420:Nervous System / Q000196:embryology / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D026801:Synteny
D018076:DNA, Complementary; C459198:IGSF9 protein, human; C486759:Igsf9 protein, mouse; D007136:Immunoglobulins; D009419:Nerve Tissue Proteins