Chondroinduction of mouse mesenchymal stem cells in three-dimensional highly porous matrix scaffolds.
Porous polyvinyl formal (PVF) resin and poly(lactide-caprolactone) [P(LA/CL)] sponges were examined as three-dimensional matrices for chondroinduction of cultured bone marrow mesenchymal stem cells (MSCs). Approximately 5 x 10(5) mouse MSCs were seeded in porous PVF resin or P(LA/CL) sponges and were cultured for up to 1 month in serum-free high-glucose Dulbecco's modified Eagle's medium containing 10 ng/mL transforming growth factor-beta3 and 100 nM dexamethasone for chondroinduction. After the 1-month culture period, the PVF resin and P(LA/CL) sponges contained approximately twice the amount of glycosaminoglycans compared with the control pellet. Safranin-O staining of PVF and P(LA/CL) after 1 month of culture revealed a cartilage-like extracellular matrix containing glycosaminoglycans and collagen. When implanted into nude mice, PVF and P(LA/CL) seeded with MSCs were found to be both biocompatible and chondroinductive. These highly porous scaffolds can maintain a large number of cells in a three-dimensional structure. Both are potentially promising for the chondroinduction of bone marrow MSCs for research and clinical applications.
Aung
Tun
T
Department of Biomedical Engineering, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki 305-8575, Japan.
Miyoshi
Hirotoshi
H
Tun
Thein
T
Ohshima
Norio
N
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D017690:Cell Transplantation; D020219:Chondrogenesis / Q000502:physiology*; D016895:Culture Media, Serum-Free; D046508:Culture Techniques / Q000379:methods*; D006025:Glycosaminoglycans / Q000378:metabolism; D008297:Male; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D011091:Polyesters / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
D001672:Biocompatible Materials; D016895:Culture Media, Serum-Free; D006025:Glycosaminoglycans; D011091:Polyesters; C062968:lactide-caprolactone copolymer