title: Expression of anthrax lethal factor gene by osmolyte induction.
abstract: The anthrax toxin consists of protective antigen (PA), lethal factor (LF) and edema factor (EF). PA mediates the entry of LF and EF to the cytosol where they exert their effects. Although PA is the major component of the vaccines against anthrax, LF has also been found to play an important role in enhancing protective immunity. We have developed an osmolyte-inducible LF expression system. The protein expression system contributed no additional amino acids to the recombinant LF making it suitable for the human vaccine trials.
authors: Centre for Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India.
authors: Aparna
authors: A
authors: Singh
authors: Samer
authors: S
authors: Singh
authors: Syed Mohsin
authors: SM
authors: Waheed
authors: Puneet
authors: P
authors: Khandelwal
authors: Rakesh
authors: R
authors: Bhatnagar
mesh_terms: D000881:Anthrax / Q000382:microbiology* / Q000517:prevention & control; D022122:Anthrax Vaccines; D000942:Antigens, Bacterial; D001408:Bacillus anthracis / Q000235:genetics* / Q000472:pathogenicity; D001427:Bacterial Toxins; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification; D002460:Cell Line; D004926:Escherichia coli; D015964:Gene Expression Regulation, Bacterial; D008264:Macrophages / Q000166:cytology / Q000382:microbiology; D009997:Osmotic Pressure; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification; D014774:Virulence
chemical_list: D022122:Anthrax Vaccines; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D002352:Carrier Proteins; D011993:Recombinant Fusion Proteins; C030325:anthrax toxin