title: Antioxidant properties of gallocatechin and prodelphinidins from pomegranate peel.
abstract: Gallocatechins and a range of prodelphinidins were purified by high performance liquid chromatography from pomegranate peel. Gallocatechin, gallocatechin-(4-8)-catechin, gallocatechin-(4-8)-gallocatechin and catechin-(4-8)-gallocatechin were all identified, purified and quantified by LC-DAD-MS and MS-MS. The antioxidant properties of these compounds were assessed using two methods: (i) inhibition of ascorbate/iron-induced peroxidation of phosphatidylcholine liposomes; and (ii) scavenging of the radical cation of 2,2-azinobis (3-ethyl-benzothiazoline-6-sulphonate, ABTS) relative to the water-soluble vitamin E analogue Trolox C (expressed as Trolox C equivalent antioxidant capacity, TEAC). The prodelphinidin dimers were potent antioxidants in the aqueous phase, being much more effective than the gallocatechin monomer. However, in the lipid phase, only one of the dimers (gallocatechin-(4-8)-catechin) was significantly more effective than the monomer in the inhibition of lipid peroxidation of phosphatidylcholine vesicles. This study represents the first report on the antioxidant properties of prodelphinidins.
authors: Phytochemicals Team, Nutrition, Health and Consumer Sciences Division, Institute of Food Research, Norwich, UK. geoff.plumb@bbsrc.ac.uk
authors: G W
authors: GW
authors: Plumb
authors: S
authors: S
authors: de Pascual-Teresa
authors: C
authors: C
authors: Santos-Buelga
authors: J C
authors: JC
authors: Rivas-Gonzalo
authors: G
authors: G
authors: Williamson
mesh_terms: D000872:Anthocyanins; D000975:Antioxidants / Q000494:pharmacology*; D001578:Benzopyrans / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D019281:Dimerization; D004305:Dose-Response Relationship, Drug; D005638:Fruit; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D050356:Lipid Metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008956:Models, Chemical; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D001578:Benzopyrans; D010936:Plant Extracts; C466030:gallocatechin-(4-8)-catechin; C466031:gallocatechin-(4-8)-gallocatechin; D002392:Catechin; C017185:delphinidin; C057580:gallocatechol