title: High pressure static fluorescence to study macromolecular structure-function.
abstract: Through some typical examples, the high pressure static fluorescence method is described. The potentiality of the intrinsic and extrinsic fluorescence probes are analyzed for structural characterizations. Special attention is given to the use of fluorescence to understand the behavior of enzymatic reactions under high pressure. The application of fluorescence polarization is also presented together with some relevant spectroscopic problems inherent in data interpretation.
authors: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai, PR China.
authors: Kangcheng
authors: K
authors: Ruan
authors: Claude
authors: C
authors: Balny
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D011312:Pressure; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013329:Structure-Activity Relationship
chemical_list: D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D011506:Proteins