title: The Sec34/Sec35p complex, a Ypt1p effector required for retrograde intra-Golgi trafficking, interacts with Golgi SNAREs and COPI vesicle coat proteins.
abstract: The Sec34/35 complex was identified as one of the evolutionarily conserved protein complexes that regulates a cis-Golgi step in intracellular vesicular transport. We have identified three new proteins that associate with Sec35p and Sec34p in yeast cytosol. Mutations in these Sec34/35 complex subunits result in defects in basic Golgi functions, including glycosylation of secretory proteins, protein sorting, and retention of Golgi resident proteins. Furthermore, the Sec34/35 complex interacts genetically and physically with the Rab protein Ypt1p, intra-Golgi SNARE molecules, as well as with Golgi vesicle coat complex COPI. We propose that the Sec34/35 protein complex acts as a tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted intra-Golgi vesicles.
authors: Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
authors: Elena S
authors: ES
authors: Suvorova
authors: Rainer
authors: R
authors: Duden
authors: Vladimir V
authors: VV
authors: Lupashin
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D022181:COP-Coated Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020755:Coat Protein Complex I / Q000235:genetics / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D047908:Intracellular Signaling Peptides and Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification; D011485:Protein Binding / Q000235:genetics; D021381:Protein Transport / Q000235:genetics*; D050600:SNARE Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000302:isolation & purification; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C112749:COG2 protein, S cerevisiae; C401396:COG3 protein, S cerevisiae; D002352:Carrier Proteins; D020755:Coat Protein Complex I; D047908:Intracellular Signaling Peptides and Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D010455:Peptides; D050600:SNARE Proteins; D029701:Saccharomyces cerevisiae Proteins; C456610:TFI3 protein, S cerevisiae; D033921:Vesicular Transport Proteins; C060755:YPT1 protein, S cerevisiae; D020691:rab GTP-Binding Proteins