title: Symbiotic and non-symbiotic expression of cgMT1, a metallothionein-like gene from the actinorhizal tree Casuarina glauca.
abstract: A clone for a type 1 metallothionein (cgMT1) was isolated from a Casuarina glauca nodule cDNA library. The corresponding gene belongs to a small family and is highly expressed in roots and nitrogen-fixing nodules, whereas low expression was observed in aerial parts of the plant. The promoter region of cgMT1 was isolated and fused to the beta-glucuronidase (gus) gene. Transgenic Casuarinaceae plants showed that the cgMT1 promoter was most active in roots and in the oldest region of the shoot. In situ hybridization indicated that in nodules cgMT1 transcript is present in mature Frankia-infected cells and in the pericycle. Possible roles for cgMT1 in symbiotic and nonsymbiotic tissues are discussed.
authors: Department of Plant Sciences, University of Cambridge, UK.
authors: Laurent
authors: L
authors: Laplaze
authors: Hassen
authors: H
authors: Gherbi
authors: Emile
authors: E
authors: Duhoux
authors: Katharina
authors: K
authors: Pawlowski
authors: Florence
authors: F
authors: Auguy
authors: Fathia
authors: F
authors: Guermache
authors: Claudine
authors: C
authors: Franche
authors: Didier
authors: D
authors: Bogusz
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006651:Histocytochemistry; D017403:In Situ Hybridization; D019684:Magnoliopsida / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000235:genetics*; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013559:Symbiosis / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D008668:Metallothionein; D005966:Glucuronidase