[Flow cytometry estimate of s phase cell cycle and prognosis of osteosarcoma in children and adolescents].
Flow cytometry analysis has been used to measure the S phase in paraffin embedded osteosarcoma tissues of 22 children and adolescents who were surgically treated. The aim of this study was the assessment of S phase cell cycle in relation to survival, localization of tumour and histopathological grading. Our findings demonstrate the usefulness of flow cytometry determination of S phase cell cycle as a prognostic factor. Higher percentage of S phase cells predict worse prognosis.
Wnuk
A
A
Samodzielna Pracownia Cytometrii, Instytut Matki i Dziecka, Kasprzaka 17a, 01-211 Warszawa, Poland. cytometr@imid.med.pl
Rytwinski
K
K
Rychlowska
M
M
Klepacka
T
T
Michalak
E
E
D000293:Adolescent; D000782:Aneuploidy; D001859:Bone Neoplasms / Q000473:pathology* / Q000601:surgery; D002648:Child; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology* / Q000601:surgery; D011379:Prognosis; D016196:S Phase
D004273:DNA, Neoplasm