Combined antibiotic and free radical trap treatment is effective at combating Staphylococcus-aureus-induced septic arthritis.
Although early antibiotic treatment of patients with septic arthritis eradicates bacteria, joint destruction commonly results from the unregulated host inflammatory responses to infection. The spin trap compound phenyl-N-tert-butyl nitrone (PBN) has been shown to have both anti-inflammatory and antioxidant effects. The aim of this study was to assess the effect of combined systemic administration of PBN and cloxacillin on the development of Staphylococcus aureus arthritis.Three days after Naval Medical Research Institute (NMRI) mice were infected intravenously with S. aureus LS-1, daily treatment was started with cloxacillin alone, PBN alone, or cloxacillin and PBN. Arthritis, weight loss and general condition were evaluated for each mouse, and joints were analyzed histopathologically. Systemic administration of PBN in conjunction with cloxacillin ameliorated the course of experimental S. aureus arthritis, as evidenced by an increased cure rate. Thus, combinatorial antioxidant plus antibiotic anti-inflammatory therapies represent a potentially efficacious approach to the management of septic arthritis.
Sakiniene
Egidija
E
Department of Rheumatology, University of Göteborg, Sweden. Egidija.Sakiniene@rheuma.gu.se
Collins
L Vincent
LV
D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000382:microbiology / Q000503:physiopathology; D001170:Arthritis, Infectious / Q000188:drug therapy* / Q000382:microbiology / Q000503:physiopathology; D003023:Cloxacillin / Q000627:therapeutic use*; D003497:Cyclic N-Oxides; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D005260:Female; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D007668:Kidney / Q000382:microbiology; D051379:Mice; D008815:Mice, Inbred Strains; D009589:Nitrogen Oxides / Q000627:therapeutic use*; D010406:Penicillins / Q000627:therapeutic use*; D012720:Severity of Illness Index; D013211:Staphylococcus aureus / Q000276:immunology; D013585:Synovitis / Q000188:drug therapy / Q000473:pathology; D016896:Treatment Outcome
D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D009589:Nitrogen Oxides; D010406:Penicillins; C029217:phenyl-N-tert-butylnitrone; D003023:Cloxacillin