Biodistribution of long-circulating PEG-liposomes in a murine model of established subcutaneous abscesses.
The biodistribution of long-circulating PEG-liposomes in a subcutaneous mouse model of established mixed infection abscesses was investigated to assess their possible role as drug carriers in the treatment of small, undrainable intra-abdominal abscesses. There was a 10-30-fold greater localisation of (67)Ga-labelled PEG-liposomes in abscesses compared to uninfected normal skin samples. Over 3% of the injected dose (ID) of liposomes was present in the abscesses 24 h after liposome administration in contrast to 0.1% in normal skin sections. The percentage ID present in the liver, spleen and kidneys was 17%, 4% and 2% per organ respectively. Five days after liposome injection, 2% ID could still be recovered from the abscesses. Using colloidal gold-labelled PEG-liposomes, it was shown that there was a 4-fold greater density of liposome clusters in the subcutaneous tissue surrounding the capsule than in the core of the abscesses. The clusters within the abscesses were distributed evenly. We conclude that PEG-liposomes localise to a significant degree at the infection focus in our mouse model and may provide a new approach to the antimicrobial treatment of intra-abdominal abscesses.
Stearne
Lorna E T
LE
Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, P.O. Box 1738, 3000 DR, Netherlands. stearne@kmic.fgg.eur.nl
Schiffelers
Raymond M
RM
Smouter
Edwin
E
Bakker-Woudenberg
Irma A J M
IA
Gyssens
Inge C
IC
D018784:Abdominal Abscess / Q000378:metabolism*; D000818:Animals; D002199:Capillary Permeability; D004195:Disease Models, Animal; D005710:Gallium Radioisotopes; D017979:Gold Colloid; D007668:Kidney / Q000378:metabolism; D008081:Liposomes / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011092:Polyethylene Glycols / Q000493:pharmacokinetics*; D012871:Skin Diseases / Q000378:metabolism; D013154:Spleen / Q000378:metabolism
D005710:Gallium Radioisotopes; D017979:Gold Colloid; D008081:Liposomes; D011092:Polyethylene Glycols