title: Preparation and application of a new ligand exchange chiral stationary phase for the liquid chromatographic resolution of alpha-amino acid enantiomers.
abstract: A new liquid chromatographic ligand exchange CSP has been prepared by covalently bonding (S)-N,N-carboxymethyl undecyl leucinol monosodium salt onto silica gel and employed in resolving various alpha-amino acids. The new CSP was quite good in resolving various a-amino acids and the resolution results were dependent on the type and content of organic modifier in the mobile phase. From these results, a chiral recognition model using a lipophilic interaction between the tethering alkyl group of the CSP and the substituent at the chiral center of alpha-amino acids was proposed. The liquid chromatographic resolution of alpha-amino acids on the new CSP was also found to be dependent on the Cu(II) concentration in the mobile phase and the column temperature.
authors: Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, South Korea. mhhyun@pusan.ac.kr
authors: Myung Ho
authors: MH
authors: Hyun
authors: Sang Cheol
authors: SC
authors: Han
authors: Cheong Wha
authors: CW
authors: Lee
authors: Yun Kyeong
authors: YK
authors: Lee
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D008024:Ligands