Synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate with recombinant Escherichia coli cells expressing (S)-specific secondary alcohol dehydrogenase.
The synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate ((R)-ECHB) from ethyl 4-chloroacetoacetate was studied using whole recombinant cells of Escherichia coli expressing a secondary alcohol dehydrogenase of Candida parapsilosis. Using 2-propanol as an energy source to regenerate NADH, the yield of (R)-ECHB reached 36.6 g/l (more than 99% ee, 95.2% conversion yield) without addition of NADH to the reaction mixture.
Yamamoto
Hiroaki
H
Tsukuba Research Center, Daicel Chemical Industries, Ltd., Ibaraki, Japan. h-yamamoto@daicel.co.jp
Matsuyama
Akinobu
A
Kobayashi
Yoshinori
Y
D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D001483:Base Sequence; D002087:Butyrates / Q000378:metabolism*; D002175:Candida / Q000201:enzymology*; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic
D002087:Butyrates; D017931:DNA Primers; C121244:ethyl 4-chloro-3-hydroxybutanoate; D000426:Alcohol Dehydrogenase