title: A bacteriophage reagent for Salmonella: molecular studies on Felix 01.
abstract: Felix 01 (F01) is a bacteriophage originally isolated by Felix and Callow which lyses almost all Salmonella strains and has been widely used as a diagnostic test for this genus. Molecular information about this phage is entirely lacking. In the present study, the DNA of the phage was found to be a double-stranded linear molecule of about 80 kb. 11.5 kb has been sequenced and in this region A + T content is 60%. There are relatively few restriction endonuclease cleavage sites in the native genome and clones show this is due to their absence rather than modification. A restriction map of the genome has been constructed. The ends of the molecule cannot be ligated although they contain 5' phosphates. At least 60% of the genome must encode proteins. In the sequenced portion, many open reading frames exist and these are tightly packed together. These have been examined for homology to published proteins but only 1 to 17 shows similarity to known proteins. F01 is therefore the prototype of a new phage family. On the basis of restriction sites, codon usage and the distribution of nonsense codons in the unused reading frames, a strong case can be made for natural selection that reacts to mRNA structure and function.
authors: Department of Biology, Technion, Israel Institute of Technology, Haifa. jkuhn@techunix.technion.ac.il
authors: Jonathan
authors: J
authors: Kuhn
authors: Mordechai
authors: M
authors: Suissa
authors: David
authors: D
authors: Chiswell
authors: Aviva
authors: A
authors: Azriel
authors: Bluma
authors: B
authors: Berman
authors: Dina
authors: D
authors: Shahar
authors: Sarah
authors: S
authors: Reznick
authors: Rekefet
authors: R
authors: Sharf
authors: Joseph
authors: J
authors: Wyse
authors: Tamar
authors: T
authors: Bar-On
authors: Ilana
authors: I
authors: Cohen
authors: Rachel
authors: R
authors: Giles
authors: Irit
authors: I
authors: Weiser
authors: Sharon
authors: S
authors: Lubinsky-Mink
authors: Shimon
authors: S
authors: Ulitzur
mesh_terms: D001483:Base Sequence; D003062:Codon; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005814:Genes, Viral; D016679:Genome, Viral; D016366:Open Reading Frames; D015183:Restriction Mapping; D012475:Salmonella / Q000821:virology; D012483:Salmonella Phages / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D014764:Viral Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D014774:Virulence
chemical_list: D003062:Codon; D004279:DNA, Viral; D014764:Viral Proteins