title: In vitro screening for antitumour activity of Clinopodium vulgare L. (Lamiaceae) extracts.
abstract: Aqueous extract of Clinopodium vulgare L. showed strong antitumour activity when tested in vitro on A2058 (human metastatic melanoma), HEp-2 (epidermoid carcinoma, larynx, human) and L5178Y (mouse lymphoma) cell lines-6 h after treatment disintegration of the nuclei and cell lysis started. Applied at a concentration of 80 microg/ml it reduced the cell survival to 1.0, 5.6 and 6.6%, respectively. The concentrations of aqueous extract inhibiting the growth of A2058, HEp-2 and L5178Y cells by 50% (IC50 values) were calculated to be 20, 10 and 17.8 microg/ml respectively. Two groups of active substances were detected: the first one, probably combining glycosides, influenced adhesion, while the second one caused massive cell vacuolisation. The chloroform extract, which contained ursolic acid and gentriacontan had also cytotoxic, however a little bit weaker effect. All changes observed were irreversible.
authors: Section for Medical Inflammation Research, Department of Cell and Molecular Biology, Lund University, Sweden. Balik.Dzhambazov@inflam.lu.se
authors: Balik
authors: B
authors: Dzhambazov
authors: Sashka
authors: S
authors: Daskalova
authors: Adriana
authors: A
authors: Monteva
authors: Nikola
authors: N
authors: Popov
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004354:Drug Screening Assays, Antitumor / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry / Q000633:toxicity*; D051379:Mice; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D010936:Plant Extracts