Minor pilin subunits are conserved in Vibrio cholerae type IV pili.
The nucleotide sequences of five open reading frames within the Vibrio cholerae NAGV14 type IV pilus gene cluster were determined. The genes showed high homology to the mannose-sensitive hemagglutinin (MSHA) pilus genes mshB, mshC, mshD, mshO and mshP. PCR analysis showed that a MSHA-like gene cluster is highly conserved among different V. cholerae strains, with the exception of the previously reported major pilin subunit. Recombinant MshB and MshO proteins were purified and specific antiserum was raised to each of them. Western blotting analyses showed that these antisera reacted with purified NAGV14 and MSHA pili. The results suggested that MshB and MshO are minor components of the pilus fiber. Although there was no cross-reaction between the major pilin subunits of NAGV14 and MSHA pili, minor components seemed to be highly homologous and immunologically cross-reactive.
Toma
Claudia
C
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan. k950417@med.u-ryukyu.ac.jp
Kuroki
Hiromi
H
Nakasone
Noboru
N
Ehara
Masahiko
M
Iwanaga
Masaaki
M
D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D003429:Cross Reactions; D004269:DNA, Bacterial / Q000235:genetics; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D005798:Genes, Bacterial; D006388:Hemagglutinins / Q000235:genetics* / Q000276:immunology; D037601:Mannose-Binding Lectin; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012703:Serotyping; D013045:Species Specificity; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006388:Hemagglutinins; D037601:Mannose-Binding Lectin; C101032:MshA protein, Vibrio cholerae; D036781:Fimbriae Proteins