In situ detection of Bartonella henselae cells.
A new in situ hybridization technique was developed for identification of Bartonella henselae cells in cell suspension or in tissue sections. Use of highly specific probes labeled with either fluorescein or digoxigenin allows discrimination between B. henselae and closely related B. quintana cells. No cross-hybridization with other Bartonella or non-Bartonella species was observed. Besides its specificity it showed higher sensitivity as compared to PCR based detection methods. Moreover, its application allows direct observation of B. henselae in infected tissues.
Hercík
K
K
Division of Cell and Molecular Microbiology, Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic.
Melter
O
O
Janecek
J
J
Branny
P
P
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods; D001474:Bartonella Infections / Q000382:microbiology; D018416:Bartonella henselae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015342:DNA Probes; D004076:Digoxigenin / Q000737:chemistry; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes; D017403:In Situ Hybridization / Q000379:methods*; D008099:Liver / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D012680:Sensitivity and Specificity; D013154:Spleen / Q000382:microbiology
D015342:DNA Probes; D005456:Fluorescent Dyes; D004076:Digoxigenin; D019793:Fluorescein