Assessment of the microbody luminal pH in the filamentous fungus Penicillium chrysogenum.
The enzymes of the penicillin biosynthetic pathway in Penicillium chrysogenum are located in different subcellular compartments. Consequently, penicillin pathway precursors and the biologically active penicillins have to cross one or more membranes. The final enzymatic step that is mediated by acyltransferase takes place in a microbody. The pH of the microbody lumen in penicillin producing cells has been determined with fluorescent probes and mutants of the green fluorescent protein and found to be slightly alkaline.
van der Lende
Ted R
TR
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
Breeuwer
Pieter
P
Abee
Tjakko
T
Konings
Wil N
WN
Driessen
Arnold J M
AJ
D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D006863:Hydrogen-Ion Concentration; D008164:Luminescent Proteins; D008830:Microbodies / Q000737:chemistry; D025282:Mycelium / Q000737:chemistry; D010408:Penicillium chrysogenum / Q000737:chemistry* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins