[Isolation of HIV-1 from infected individuals in Fujian Province].
OBJECTIVE
To isolate the virus from HIV-1 infected individuals.


METHODS
Blood specimens were collected from 10 HIV-1 infected persons in Fujian Province. Peripheral blood mononuclear cells (PBMCs) were separated from the heparinized blood. Each sample was cocultured with fresh normal PBMCs to isolate HIV-1. Supernatant of the cocultures was tested by using HIV-1 P24 antigen Kit.


RESULTS
Of the 10 specimens, 9 showed positive for the P24. 4 of 9 isolated infected MT4 cells, and cell syncytias could be seen. Three isolates showed transient infection of MT4, which probably belong to slow/low virus. Only one isolate could infect MT4 successively, which had been passed for 15 generations without significantly changed viral titer; it is believed to be a rapid/high replication virus.


CONCLUSIONS
HIV-1 virus was successfully isolated.
Wang
H
H
Fujian Provincial Antiepidemic Station, Fuzhou, 350001, China.
Yan
Y
Y
Chen
G
G
D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans