title: New cytogenetic variant, insertion (15;17)(q22;q12q21), in an adolescent with acute promyelocytic leukemia.
abstract: We present the case of a 15-year-old female with acute promyelocytic leukemia and a new variant chromosome rearrangement identified as ins(15;17)(q22;q12q21) by conventional cytogenetic analysis. This finding was confirmed by fluorescence in situ hybridization using the PML-RARA DNA probe and whole chromosome paints 15 and 17. A typical PML-RARA fusion transcript consistent with a breakpoint in intron 3 of the PML gene and intron 2 of the RARA gene was identified by reverse transcription polymerase chain reaction.
authors: Division of Molecular Diagnostics, Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
authors: Raj
authors: R
authors: Rolston
authors: Karen E
authors: KE
authors: Weck
authors: Jean M
authors: JM
authors: Tersak
authors: Maureen E
authors: ME
authors: Sherer
authors: Kathleen
authors: K
authors: Cumbie
authors: Sofia
authors: S
authors: Shekhter-Levin
mesh_terms: D000293:Adolescent; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic