title: Inward rectifier K(+) current under physiological cytoplasmic conditions in guinea-pig cardiac ventricular cells.
abstract: The outward current that flows through the strong inward rectifier K(+) (K(IR)) channel generates I(K1), one of the major repolarizing currents of the cardiac action potential. The amplitude and the time dependence of the outward current that flows through K(IR) channels is determined by its blockage by cytoplasmic cations such as polyamines and Mg(2+). Using the conventional whole-cell recording technique, we recently showed that the outward I(K1) can show a time dependence during repolarization due to competition of cytoplasmic particles for blocking K(IR) channels. We used the amphotericin B perforated patch-clamp technique to measure the physiological amplitude and time dependence of I(K1) during the membrane repolarization of guinea-pig cardiac ventricular myocytes. In 5.4 mM K(+) Tyrode solution, the density of the current consisting mostly of the sustained component of the outward I(K1) was about 3.1 A F(-1) at around -60 mV. The outward I(K1) showed an instantaneous increase followed by a time-dependent decay (outward I(K1) transient) on repolarization to -60 to -20 mV subsequent to a 200 ms depolarizing pulse at +37 mV (a double-pulse protocol). The amplitudes of the transients were large when a hyperpolarizing pre-pulse was applied before the double-pulse protocol, whereas they were small when a depolarizing pre-pulse was applied. The peak amplitudes of the transients elicited using a hyperpolarizing pre-pulse were 0.36, 0.63 and 1.01 A F(-1), and the decay time constants were 44, 14 and 6 ms, at -24, -35 and -45 mV, respectively. In the current-clamp experiments, a phase-plane analysis revealed that application of pre-pulses changed the current density at the repolarization phase to the extents expected from the changes of the I(K1) transient. Our study provides the first evidence that an outward I(K1) transient flows during cardiac action potentials.
authors: Department of Physiology, Saga Medical School, Saga 849-8501, Japan. keiko@post.saga-med.ac.jp
authors: Keiko
authors: K
authors: Ishihara
authors: Ding-Hong
authors: DH
authors: Yan
authors: Shintaro
authors: S
authors: Yamamoto
authors: Tsuguhisa
authors: T
authors: Ehara
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D003593:Cytoplasm / Q000502:physiology; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology*; D006352:Heart Ventricles; D066298:In Vitro Techniques; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D024661:Potassium Channels, Inwardly Rectifying / Q000187:drug effects / Q000502:physiology*
chemical_list: D024661:Potassium Channels, Inwardly Rectifying; D000666:Amphotericin B