Study of the enantioselective elimination of four toxaphene congeners in rat after intravenous administration by high resolution gas chromatography negative ion mass spectrometry.
This study was performed to investigate the possible enantioselective metabolism of the four chlorinated bornanes: #26, #32, #50 and #62 (according to the Parlar nomenclature) by rats. Rats were exposed to a mixture of these toxaphenes by a single intravenous injection. Enantiomer ratios (ER) as well as the enantiomer fractions (EF) were determined in brain, adipose tissue and liver samples at six time intervals by high resolution gas chromatography (HRGC) coupled to negative ion chemical ionization (NICI) mass spectrometry (MS). Capillaries coated with heptakis-(2,3,6-O-tert-butyldimethylsilyl)-beta-cyclodextrin (TBDMS-CD) or octakis-(2,3,6-tri-O-ethyl)-gamma-cyclodextrin (TEG-CD) were used for the enantioselective separations. Significant time-dependent changes of ER and EF were found in all the three tissues for #26, #50 and #62. Greatest deviations from racemic composition were found in the liver, which is known to be the major metabolizing organ for toxaphenes. #32 was metabolized the fastest, but showed no changes in ER. Brief information is also included about the possible reasons for the different behaviors of the four congeners in the studied tissues.
Skopp
S
S
Organic Analytical Chemistry, University of Basel, Switzerland.
Oehme
M
M
Drenth
H
H
D000818:Animals; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007275:Injections, Intravenous; D007306:Insecticides / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism; D014112:Toxaphene / Q000031:analogs & derivatives / Q000493:pharmacokinetics*
D007306:Insecticides; D014112:Toxaphene