Search for genes positively selected during primate evolution by 5'-end-sequence screening of cynomolgus monkey cDNAs.
It is possible to assess positive selection by using the ratio of K(a) (nonsynonymous substitutions per plausible nonsynonymous sites) to K(s) (synonymous substitutions per plausible synonymous sites). We have searched candidate genes positively selected during primate evolution by using 5'-end sequences of 21,302 clones derived from cynomolgus monkey (Macaca fascicularis) brain cDNA libraries. Among these candidates, 10 genes that had not been shown by previous studies to undergo positive selection exhibited a K(a)/K(s) ratio > 1. Of the 10 candidate genes we found, 5 were included in the mitochondrial respiratory enzyme complexes, suggesting that these nuclear-encoded genes coevolved with mitochondrial-encoded genes, which have high mutation rates. The products of other candidate genes consisted of a cell-surface protein, a member of the lipocalin family, a nuclear transcription factor, and hypothetical proteins.
Osada
Naoki
N
Division of Genetic Resources, National Institute of Infectious Diseases, Tokyo, Japan.osada@nih.go.jp
Kusuda
Jun
J
Hirata
Makoto
M
Tanuma
Reiko
R
Hida
Munetomo
M
Sugano
Sumio
S
Hirai
Momoki
M
Hashimoto
Katsuyuki
K
D000818:Animals; D003574:Cytochrome c Group / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D030561:Databases, Nucleic Acid; D003576:Electron Transport Complex IV / Q000235:genetics; D019143:Evolution, Molecular; D005796:Genes / Q000235:genetics; D008252:Macaca fascicularis / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011323:Primates / Q000235:genetics*; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA
D003574:Cytochrome c Group; D018076:DNA, Complementary; D003576:Electron Transport Complex IV