Characterization of plasmid MIP233 (IncHI3) of the H complex.
Plasmid MIP233, the unique member of the IncHI3 subgroup of the H incompatibility complex, is a large self-transferable plasmid encoding for sucrose utilization, the PacB character and resistance to tellurite. The current study examined whether pMIP233 conferred other putatively advantageous plasmid-encoded functions. Heavy metal studies revealed pMIP233 phenotypes of resistance to cadmium, cobalt, lead, zinc and nickel. Sequencing of random clones from a pMIP233 library revealed extensive homology with IS elements and genes involved in the metabolic processes demonstrated. Interestingly, pMIP233 did not appear to code for resistance to a wide range of antibiotics. pMIP233 is considered to be a metabolic plasmid.
Alonso
Guillermina
G
Instituto de Biología Experimental, Facultad de Ciencias, Universidad Central de Venezuela, Caracas. galonso@reacciun.ve
Vílchez
Glenda
G
Bruzual
Igor
I
Rodríguez-Lemoine
Vidal
V
D001419:Bacteria / Q000187:drug effects; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics*; D008670:Metals / Q000494:pharmacology*; D016366:Open Reading Frames; D010957:Plasmids; D013395:Sucrose / Q000378:metabolism
D004251:DNA Transposable Elements; D008670:Metals; D013395:Sucrose