Near-infrared analysis of soluble and insoluble dietary fiber fractions of cereal food products.
The use of near-infrared (NIR) reflectance spectroscopy for the rapid and accurate measurement of soluble and insoluble dietary fiber was explored in a diverse group of cereal products. Ground samples were analyzed for soluble and insoluble dietary fiber (AOAC Method 991.43) and scanned (NIRSystems 6500 monochromator) to obtain NIR spectra. Modified PLS models were developed to predict insoluble and soluble dietary fiber using data sets expanded to include products with high fat and high sugar contents. The models predicted insoluble dietary fiber accurately with an SECV of 1.54% and an R(2) of 0.98 (AOAC determined range of 0-48.77%) and soluble dietary fiber less accurately with an SECV of 1.15% and an R(2) of 0.82 (AOAC determined range of 0-13.84%). Prediction of independent validation samples by the soluble fiber model resulted in a bias that may be related to the way the reference method treats samples with different soluble fiber constituents. The insoluble fiber model can be used to rapidly monitor insoluble dietary fiber in cereal products for nutrition labeling.
Kays
Sandra E
SE
Richard B. Russell Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 5677, Athens, Georgia 30604-5677, USA. sekays@qaru.ars.usda.gov
Barton
Franklin E
FE
D002138:Calibration; D004043:Dietary Fiber / Q000032:analysis*; D002523:Edible Grain / Q000737:chemistry*; D011786:Quality Control; D012995:Solubility; D019265:Spectroscopy, Near-Infrared