Activation of cellulose membranes with 1,1'-carbonyldiimidazole or 1-cyano-4-dimethylaminopyridinium tetrafluoroborate as a basis for the development of immunosensors.
Methods for the activation of a cellulose dialysis membrane for immunosensor applications have been developed. For activation two reagents, 1,1'-carbonyldiimidazole (CDI) and 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP), were compared with respect to the coupling efficiency for glucose oxidase (GOx) and 1,8-diamino-2,6-dioxaoctane. The maximum level of activation was 2.4 micromol cm(-2) for CDI and 0.2 micromol cm(-2) for CDAP activation. We observed 1.5 microg cm(-2) and 0.4 x 10(-4) U cm(-2) GOx with CDI-activated membranes whereas 1.7 microg cm(-2) and 7.2 x 10(-4) U cm(-2) GOx were observed with CDAP-activated membranes. With 1,8-diamino-2,6-dioxaoctane amino group densities of 0.165 and 0.09 micromol cm(-2) were observed via CDI and CDAP activation, respectively. An amino-modified membrane was used for coupling a ligand (pentapeptide) and an immunoenzymometric assay for hemoglobin A1c was carried out.
Stöllner
Daniela
D
Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25, Haus 25, D-14476 Golm, Germany.
Scheller
Frieder W
FW
Warsinke
Axel
A
D000345:Affinity Labels / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002482:Cellulose / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006442:Glycated Hemoglobin / Q000032:analysis / Q000276:immunology; D007093:Imidazoles / Q000737:chemistry*; D007124:Immunoenzyme Techniques / Q000379:methods; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D008970:Molecular Weight; D009570:Nitriles / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D011726:Pyridinium Compounds / Q000737:chemistry*
D000345:Affinity Labels; D002482:Cellulose; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D006442:Glycated Hemoglobin; D007093:Imidazoles; D008567:Membranes, Artificial; D009570:Nitriles; D011725:Pyridines; D011726:Pyridinium Compounds; C417980:4-(dimethylamine)pyridine; C067292:1-cyano-4-dimethylaminopyridinium; C006900:N,N-carbonyldiimidazole