Comparative genome sequencing for discovery of novel polymorphisms in Bacillus anthracis.
Comparison of the whole-genome sequence of Bacillus anthracis isolated from a victim of a recent bioterrorist anthrax attack with a reference reveals 60 new markers that include single nucleotide polymorphisms (SNPs), inserted or deleted sequences, and tandem repeats. Genome comparison detected four high-quality SNPs between the two sequenced B. anthracis chromosomes and seven differences among different preparations of the reference genome. These markers have been tested on a collection of anthrax isolates and were found to divide these samples into distinct families. These results demonstrate that genome-based analysis of microbial pathogens will provide a powerful new tool for investigation of infectious disease outbreaks.
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Timothy D
TD
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA., Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA.
Salzberg
Steven L
SL
Pop
Mihai
M
Shumway
Martin
M
Umayam
Lowell
L
Jiang
Lingxia
L
Holtzapple
Erik
E
Busch
Joseph D
JD
Smith
Kimothy L
KL
Schupp
James M
JM
Solomon
Daniel
D
Keim
Paul
P
Fraser
Claire M
CM
D000818:Animals; D000881:Anthrax / Q000382:microbiology; D001408:Bacillus anthracis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D023482:Bioterrorism; D007446:Chromosome Inversion; D019295:Computational Biology; D004196:Disease Outbreaks; D005819:Genetic Markers; D014644:Genetic Variation; D016680:Genome, Bacterial; D023281:Genomics; D006801:Humans; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010802:Phylogeny; D010957:Plasmids; D020641:Polymorphism, Single Nucleotide; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D013045:Species Specificity; D019895:Transposases / Q000235:genetics; D014774:Virulence / Q000235:genetics
D005819:Genetic Markers; D019895:Transposases