title: A locust DNA-binding protein involved in gene regulation by juvenile hormone.
abstract: Although juvenile hormone (JH) has essential roles in insect development and reproduction, the molecular mechanisms of gene regulation by JH remain an enigma. In Locusta migratoria, the partially palindromic 15-nt sequence, GAGGTTCGAG(A)/(T)CCT(T)/(C), found upstream of a JH-induced gene, jhp21, was designated as a putative juvenile hormone response element (JHRE). When JH-deprived adult female locusts were treated with the active JH analog, methoprene, a fat body nuclear factor that bound specifically to JHRE appeared after 24 h. Binding exhibited a preference for an inverted repeat with GAGGTTC in the left half-site, a single nucleotide spacer, and a right half-site in which some variation is acceptable. Binding to JHRE was abolished by phosphorylation catalyzed by a C-type protein kinase present in the nuclear extracts. The DNA-binding protein is thus believed to be a transcription factor, which is brought to an active state through the action of JH and then participates in the regulation of certain JH-dependent genes.
authors: Department of Biology, Queen's University, Kingston, Ont., Canada K7L 3N6.
authors: S
authors: S
authors: Zhou
authors: J
authors: J
authors: Zhang
authors: M
authors: M
authors: Hirai
authors: Y
authors: Y
authors: Chinzei
authors: H
authors: H
authors: Kayser
authors: G R
authors: GR
authors: Wyatt
authors: V K
authors: VK
authors: Walker
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005216:Fat Body / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006110:Grasshoppers / Q000502:physiology*; D019476:Insect Proteins / Q000378:metabolism*; D007605:Juvenile Hormones / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D008726:Methoprene / Q000494:pharmacology; D010766:Phosphorylation; D011485:Protein Binding; D020218:Response Elements / Q000235:genetics; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D019476:Insect Proteins; D007605:Juvenile Hormones; D014157:Transcription Factors; D008726:Methoprene