PrrC from Rhodobacter sphaeroides, a homologue of eukaryotic Sco proteins, is a copper-binding protein and may have a thiol-disulfide oxidoreductase activity.
PrrC from Rhodobacter sphaeroides provides the signal input to a two-component signal transduction system that senses changes in oxygen tension and regulates expression of genes involved in photosynthesis (Eraso, J.M. and Kaplan, S. (2000) Biochemistry 39, 2052-2062; Oh, J.-I. and Kaplan, S. (2000) EMBO J. 19, 4237-4247). It is also a homologue of eukaryotic Sco proteins and each has a C-x-x-x-C-P sequence. In mitochondrial Sco proteins these cysteines appear to be essential for the biogenesis of the CuA centre of respiratory cytochrome oxidase. Overexpression and purification of a water-soluble and monomeric form of PrrC has provided sufficient material for a chemical and spectroscopic study of the properties of the four cysteine residues of PrrC, and its ability to bind divalent cations, including copper. PrrC expressed in the cytoplasm of Escherichia coli binds Ni2+ tightly and the data are consistent with a mononuclear metal site. Following removal of Ni2+ and formation of renatured metal-free rPrrC (apo-PrrC), Cu2+ could be loaded into the reduced form of PrrC to generate a protein with a distinctive UV-visible spectrum, having absorbance with a lambda(max) of 360 nm. The copper:PrrC ratio is consistent with the presence of a mononuclear metal centre. The cysteines of metal-free PrrC oxidise in the presence of air to form two intramolecular disulfide bonds, with one pair being extremely reactive. The cysteine thiols with extreme O2 sensitivity are involved in copper binding in reduced PrrC since the same copper-loaded protein could not be generated using oxidised PrrC. Thus, it appears that PrrC, and probably Sco proteins in general, could have both a thiol-disulfide oxidoreductase function and a copper-binding role.
McEwan
Alastair G
AG
School of Molecular and Microbial Sciences, The University of Queensland, 4072, Brisbane, Qld, Australia. mcewan@mailbox.uq.edu.au
Lewin
Allison
A
Davy
Sharon L
SL
Boetzel
Ruth
R
Leech
Andrew
A
Walker
Daniel
D
Wood
Tania
T
Moore
Geoffrey R
GR
D020816:Amino Acid Motifs; D000818:Animals; D001051:Apoenzymes / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005057:Eukaryotic Cells / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000737:chemistry; D011490:Protein Disulfide Reductase (Glutathione) / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012242:Rhodobacter sphaeroides / Q000201:enzymology; D012260:Ribonucleases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014867:Water / Q000737:chemistry
D001051:Apoenzymes; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D024101:Mitochondrial Proteins; D011994:Recombinant Proteins; C023548:copper-binding protein; D014867:Water; D003300:Copper; D011490:Protein Disulfide Reductase (Glutathione); C084965:PrrC protein, E coli; D012260:Ribonucleases; D003545:Cysteine