title: PspE (phage-shock protein E) of Escherichia coli is a rhodanese.
abstract: The psp (phage-shock protein) operon of Escherichia coli is induced when the bacteria are infected by filamentous phage and under several other stress conditions. The physiological role of the individual Psp proteins is still not known. We demonstrate here that the last gene of the operon, pspE, encodes a thiosulfate:cyanide sulfurtransferase (EC 2.8.1.1; rhodanese). Kinetic analysis revealed that catalysis occurs via a double displacement mechanism as described for other rhodaneses. The K(m)s for SSO3(2-) and CN- were 4.6 and 27 mM, respectively.
authors: Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands. h.adams@bio.uu.nl
authors: Hendrik
authors: H
authors: Adams
authors: Wieke
authors: W
authors: Teertstra
authors: Margot
authors: M
authors: Koster
authors: Jan
authors: J
authors: Tommassen
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000502:physiology*; D004926:Escherichia coli / Q000201:enzymology*; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000502:physiology*; D007700:Kinetics; D008969:Molecular Sequence Data; D009876:Operon; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D013884:Thiosulfate Sulfurtransferase / Q000737:chemistry / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; C069816:phage shock protein, Bacteria; D013884:Thiosulfate Sulfurtransferase