Derivative matrix isopotential synchronous fluorescence spectroscopy for the direct determination of 1-hydroxypyrene as a urinary biomarker of exposure to polycyclic aromatic hydrocarbons.
Urinary 1-hydroxypyrene is a biomarker in the measurement of human exposure to polycyclic aromatic hydrocarbons. A rapid and simple derivative isopotential synchronous fluorescence method was developed for the direct determination of 1-hydroxypyrene in urine. A length of iso-intensity route was scanned on the three-dimensional fluorescence spectrum of urine and this result was combined with that from derivative technique. Thus the strong background signals of urine were removed and the 1-hydroxypyrene can be determined directly in urine without tedious pre-separation. The derivative isopotential synchronous fluorescence spectrum was directly obtained from a single scan on a spectrofluorometer, which further simplified isopotential synchronous fluorescence technique. The recoveries of 93% to 115% were obtained for 1-hydroxypyrene added to urine.
Li
Y Q
YQ
Department of Chemistry, Xiamen University, China. yqlig@xmu.edu.cn
Sui
W
W
Wu
C
C
Yu
L J
LJ
D015415:Biomarkers; D002021:Buffers; D002138:Calibration; D004781:Environmental Exposure; D006801:Humans; D007202:Indicators and Reagents; D009153:Mutagens / Q000032:analysis* / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D011721:Pyrenes / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014556:Urine / Q000737:chemistry
D015415:Biomarkers; D002021:Buffers; D007202:Indicators and Reagents; D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; C033146:1-hydroxypyrene