Development and validation of a plasma assay for acyclovir using high-performance capillary electrophoresis with sample stacking.
A sensitive plasma assay for acyclovir has been developed and validated. Acyclovir was separated from plasma components using Oasis HLB columns. Separation was obtained with no plasma interference using micellar electrokinetic chromatography (175 mM SDS) and hydroxypropyl-beta-cyclodextrin (100 mM) in 90 mM borate buffer (pH 8.8) containing 0.2% NaCl. High sensitivity was achieved by large volume sample introduction and stacking. The linear range was from 20 to 10000 ng/ml with a limit of quantitation of 20 ng/ml. This method is a viable alternative to HPLC because of its high separation and sensitivity, reproducibility, and adaptability to other nucleoside analogs.
Vo
Hung C
HC
Preclinical Research, Viridae Clinical Sciences, Inc., 1134 Burrard Street, Vancouver, British Columbia V6Z 1Y8, Canada. hung_vo@viridae.com
Henning
Paul A
PA
Leung
Daniel T
DT
Sacks
Stephen L
SL
D000212:Acyclovir / Q000097:blood*; D000998:Antiviral Agents / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy; D012680:Sensitivity and Specificity
D000998:Antiviral Agents; D000212:Acyclovir