Comparison of murine Epo ELISA and Epo bioassays in detecting serum Epo levels during anemia associated with malaria infection.
A highly sensitive sandwich ELISA specific for murine erythropoietin (mEpo) was developed using commercially available monoclonal anti-mouse Epo antibody and polyclonal anti-human Epo antibody. This newly developed Epo ELISA protocol and the traditional Epo bioassay method were used to analyze Epo production in response to anemia induced during blood-stage Plasmodium chabaudi AS (P. chabaudi AS) malaria infection in C57BL/6 mice. Both methods revealed an inverse correlation between the serum Epo concentration and hematocrit level, but Epo values estimated by the Epo bioassay were between 5- and 20-fold higher than those estimated by the ELISA. Further study demonstrated that the estimated Epo level in bioassay was strongly influenced by other cytokines present in the samples. Therefore, the Epo bioassay detects the net erythropoiesis promoting activities, whereas the ELISA method specifically measures the level of Epo in the samples. Combined with the Epo bioassay, the murine Epo ELISA will be an extremely useful tool in specifically measuring the Epo response and facilitating the understanding of mechanisms involved in the development of anemia-associated diseases using mouse models.
Chang
Kai-Hsin
KH
Institute of Parasitology, Macdonald Campus, McGill University, 21, 111 Lakeshore Road, Ste-Anne-de-Bellevue, Quebec, Canada H9X 1C9.
Stevenson
Mary M
MM
D000740:Anemia / Q000097:blood* / Q000209:etiology; D000818:Animals; D001681:Biological Assay; D004797:Enzyme-Linked Immunosorbent Assay; D004921:Erythropoietin / Q000032:analysis* / Q000276:immunology; D008288:Malaria / Q000097:blood / Q000150:complications*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016788:Plasmodium chabaudi; D012680:Sensitivity and Specificity
D004921:Erythropoietin