A comparative thermodynamic study for both natural and artificial RNA/DNA-protein binary complexes.
In recent years, Systematic Evolution of Ligands by EXponential enrichment (SELEX) technique has been developed into a fast growing field. In contrast to traditional recognition elements, like antibody, our interests focus on novel molecular recognition elements based on nucleic acids, which are of value both for the therapy and biosensors. A comparative study of thermodynamic for both natural and artificial RNA/DNA-protein complexes would establish bases for a specificity of complex formation. In particular, we have shown that aptamers could be used for a direct measuring of thrombin enzymatic activity in a solution.
Spiridonova
V
V
A.N. Belozersky Institute of Physico-Chemical Biolog, Laboratory Building "A", and Chemistry Department, Moscow State University, Vorobyevy Gory, 119899 Moscow, Russian Federation. Spiridon@genebee.msu.su
Rassokhin
T
T
Golovin
A
A
Petrova
E
E
Rohzdestvensky
T
T
Pakhomova
Yu
Y
Kopylov
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011506:Proteins / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013816:Thermodynamics
D011506:Proteins; D012313:RNA; D004247:DNA