Occurrence and genetic association of selected virulence factors in clinical Escherichia coli isolates.
Occurrence of cnf1+ E. coli pathogenic strains among extraintestinal E. coli isolates was evaluated to explain an impact of cytotoxic necrotizing factor type 1 (CNF1) in human infections. A total of 120 E. coli isolates were characterized for presence of virulence factors cnf1- and pap--specific sequences by PCR, and the production of alpha-hemolysin using blood agar-plate test. Different association patterns among the detected virulence factors were obtained by comparison of various groups of clinical E. coli isolates. These differences probably reflect a potential impact of CNF1 in the colonization of vaginal environment.
Bogyiová
E
E
Institute of Medical Microbiology, School of Medicine, P. J. Safárik University, 040 66 Kosice, Slovakia. bogyie@central.medic.upjs.sk
Siegfried
L
L
Kmetová
M
M
Sándorcínová
Z
Z
Liptáková
A
A
Biros
E
E
D000328:Adult; D001427:Bacterial Toxins / Q000235:genetics* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003603:Cytotoxins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D010861:Fimbriae, Bacterial / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014774:Virulence / Q000235:genetics
D001427:Bacterial Toxins; D003603:Cytotoxins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; C060296:cytotoxic necrotizing factor type 1