Antioxidant activity of phenolic compounds isolated from Mesona procumbens Hemsl.
The antioxidant activity of phenolic compounds isolated from Mesona procumbens Hemsl. (Hsian-tsao) was investigated. Hsian-tsao was extracted with various solvents, and the results showed that the fraction treated with acidic ethyl acetate (pH 2) possessed large amounts of phenolic compounds and a strong antioxidant activity on peroxidation of linoleic acid. The antioxidant activity (inhibition of peroxidation, IP%) of the acidic ethyl acetate of Hsian-tsao extract at 50 microg/mL (98.9%) was stronger than those of 50 microg/mL alpha-tocopherol (78%) and BHA at 10 microg/mL (90%). When fractionated with Amberlite XAD-7 gel chromatography, the acidic ethyl acetate fraction of Hsian-tsao extract was separated into four subfractions (A-D). Subfraction B, with high yield and strong antioxidant activity, was further isolated and purified and then identified as containing protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, caffeic acid, and syringic acid by means of UV, EI-MS, and (1)H and (13)C NMR. The antioxidant capability of isolated compounds was also determined using the thiocyanate system and the erythrocyte ghost system. The results indicate that the phenolic acids could be important antioxidant components in Hsian-tsao, among which caffeic acid with the highest antioxidant activity and the greatest content is most important.
Hung
Chien-Ya
CY
Department of Food Science, National Chung Hsing University, 250 Kuokuang Road, Taichung 402, Taiwan, Republic of China.
Yen
Gow-Chin
GC
D000085:Acetates; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002109:Caffeic Acids / Q000032:analysis / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D062385:Hydroxybenzoates / Q000032:analysis; D019787:Linoleic Acid / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D014641:Vanillic Acid / Q000032:analysis; D024502:alpha-Tocopherol / Q000494:pharmacology
D000085:Acetates; D000975:Antioxidants; D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; D010636:Phenols; D010936:Plant Extracts; C009091:protocatechuic acid; D005707:Gallic Acid; C007650:ethyl acetate; D019787:Linoleic Acid; C001945:syringic acid; D014641:Vanillic Acid; D024502:alpha-Tocopherol; C040048:caffeic acid