title: Humanin inhibits cell death of serum-deprived PC12h cells.
abstract: Humanin (HN) and S14G HN (HNG) are recently discovered polypeptides that rescue cells from death induced by multiple different types of familial Alzheimer's disease genes and by amyloid-beta. However, the cytoprotective activity of these peptides against other cell death-inducing stimuli remains unclear. In this study, we demonstrated, using three different methods (MTS assay, caspase-3 assay, and detection of DNA fragmentation), that both HN and HNG protect PC12 cells from death elicited by serum deprivation. This implies the potential of the peptides to rescue cells from a broad spectrum, if not all, of cell death-inducing factors. Further investigations on HN may lead the possible application of this peptide as therapeutic agent for the treatment of other neurodegenerative diseases.
authors: Department of Neurology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan.
authors: Shingo
authors: S
authors: Kariya
authors: Nobuyuki
authors: N
authors: Takahashi
authors: Naoki
authors: N
authors: Ooba
authors: Makoto
authors: M
authors: Kawahara
authors: Hitoshi
authors: H
authors: Nakayama
authors: Satoshi
authors: S
authors: Ueno
mesh_terms: D000818:Animals; D053148:Caspase 3; D020169:Caspases / Q000187:drug effects / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D016895:Culture Media, Serum-Free / Q000633:toxicity*; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D047908:Intracellular Signaling Peptides and Proteins; D019636:Neurodegenerative Diseases / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology*; D051381:Rats
chemical_list: D016895:Culture Media, Serum-Free; D047908:Intracellular Signaling Peptides and Proteins; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D011506:Proteins; C427211:humanin; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases