title: DNA microarray assessment of putative Borrelia burgdorferi lipoprotein genes.
abstract: A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. These data show that a DNA microarray can globally examine the genes encoding B. burgdorferi lipoproteins.
authors: Section of Rheumatology, Department of Internal Medicine, School of Medicine, Yale University, New Haven, Connecticut 06520-8031, USA.
authors: Fang Ting
authors: FT
authors: Liang
authors: F Kenneth
authors: FK
authors: Nelson
authors: Erol
authors: E
authors: Fikrig
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D025065:Borrelia burgdorferi / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D008074:Lipoproteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008074:Lipoproteins