title: Juvenile hormone biosynthesis in the fall armyworm, Spodoptera frugiperda (Lepidoptera, Noctuidae).
abstract: The in vitro production of juvenile hormones (JH) was investigated by using corpora allata (CA) of larvae and corpora cardiaca-corpora allata (CC-CA) complexes of adult females of the fall armyworm Spodoptera frugiperda. In female moths, JH release was high compared to that in 5th and 6th instar larvae. Concentrations of 0.11-0.12 mM methionine, 180-200 mM Na(+), 5.8-8.3 mM K(+), 10-50 mM Ca(2+) and a pH range of 5.7-6.3 yielded optimal incorporation of L-[methyl-(3)H] methionine in vitro by CC-CA complexes. The highest hourly incorporation occurred during a 9-h incubation period following a 1.5-h lag-phase. JH release from CC-CA complexes of adult females was shown to be age-dependent with a peak value on day 2 (approx. 4 pmol h(-1) CA(-1)). By a combination of reversed phase (RP)- and normal phase (NP)-high performance liquid chromatography (HPLC), two major labelled products released by the complex were separated. One compound co-migrated with chemically synthesized JH II diol, the second compound with JH III diol. Only traces of JH II and III could be detected in some samples. Gland extracts also contained both the major radiolabelled products. Double labelling experiments using [3H]methionine and [14C]acetate confirmed their de novo synthesis in CC-CA complexes of female moths. The nature of chemically synthesized reference JH III diol was proved by LC-MS (ESI mass spectrometry) and 1H-NMR (nuclear magnetic resonance spectroscopy).
authors: Animal Ecology I, University of Bayreuth, D-95440 Bayreuth, Germany.
authors: Sebastian
authors: S
authors: Range
authors: Uwe
authors: U
authors: Oeh
authors: Matthias W
authors: MW
authors: Lorenz
authors: Winfried
authors: W
authors: Etzel
authors: Ralf
authors: R
authors: Nauen
authors: Klaus H
authors: KH
authors: Hoffmann
mesh_terms: D000367:Age Factors; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003335:Corpora Allata / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D007605:Juvenile Hormones / Q000096:biosynthesis*; D009682:Magnetic Resonance Spectroscopy; D009490:Neurosecretory Systems / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D018411:Spodoptera / Q000378:metabolism*; D013997:Time Factors
chemical_list: D007605:Juvenile Hormones