14-3-3 in the cerebrospinal fluid of patients with variant and sporadic Creutzfeldt-Jakob disease measured using capture assay able to detect low levels of 14-3-3 protein.
A protein capture assay was used to measure 14-3-3 (-isoform) in the cerebrospinal fluid (CSF) of patients with either variant or sporadic Creutzfeldt-Jakob disease (CJD). The results were compared with those obtained using Western blotting. Elevated levels of 14-3-3 were found in 58% of variant CJD (vCJD) patients and 82% of sporadic CJD (spCJD) patients using the protein capture assay. Using a Western blotting technique, the presence of CSF 14-3-3 was detected in 58% of vCJD patients and in 89% of spCJD patients. When the results from the protein capture assay and the Western blot were combined, 14-3-3 was detected in 77% of vCJD patients and in 91% of spCJD patients. These results suggest that although analysis of CSF 14-3-3 is not as useful in vCJD as it is in the sporadic form of the disease, a combination of these two techniques results in increased sensitivity of 14-3-3 for the diagnosis of vCJD.
Green
Alison J E
AJ
National CJD Surveillance Unit, University of Edinburgh, Western General Hospital, Crewe Road, EH4 2XU, UK. alison.green@ed.ac.uk
Ramljak
Sanja
S
Müller
Werner E G
WE
Knight
Richard S G
RS
Schröder
Heinz C
HC
D048948:14-3-3 Proteins; D000368:Aged; D020816:Amino Acid Motifs / Q000502:physiology; D001681:Biological Assay / Q000379:methods; D015415:Biomarkers; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid* / Q000175:diagnosis* / Q000503:physiopathology; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D013223:Statistics as Topic; D014446:Tyrosine 3-Monooxygenase / Q000134:cerebrospinal fluid*; D015854:Up-Regulation / Q000502:physiology*
D048948:14-3-3 Proteins; D015415:Biomarkers; D014446:Tyrosine 3-Monooxygenase