Binding of vanadate to human albumin in infusion solutions, to proteins in human fresh frozen plasma, and to transferrin.
The binding of vanadate (V) to human serum albumin (HSA) in infusion solutions, to human fresh frozen plasma (FFP), and to human transferrin (TF) was investigated over a wide concentration range. Free V concentrations were obtained by ultrafiltration. Total and free V concentrations were determined using electrothermal atomic absorption spectrometry (ETAAS). Binding parameters were obtained by non-linear regression. V only bound appreciably to HSA at low concentrations (<1 microM). The binding capacity of HSA was about 1000-fold lower than that of FFP and TF per mole of protein. Binding to FFP and TF in the concentration range investigated could be described by a combination of saturable and additional non-saturable binding. The respective maximal binding capacities (B(max), microM), dissociation constants (k(D), microM), and proportionality constants (C) for the non-saturable, linear binding were B(max)=27, k(D)=2.5, C=0.19 for FFP and B(max)=47, k(D)=0.47, C=0.38 for TF. The results suggest that V is predominantly bound to transferrin in FFP. It is concluded that HSA in infusion solutions represents a reservoir of readily accessible V. Nevertheless, given the high binding capacity of transferrin in plasma, the amount of vanadate delivered via the brief administration of HSA solutions is unlikely to be of major importance.
Heinemann
Günter
G
Institut für Laboratoriumsmedizin, Deutsches Herzzentrum München des Freistaates Bayern, Klinik an der Technischen Universität, Lazarettstrasse 36, 80636 Munich, Germany. heinemann@dhm.mhn.de
Fichtl
Burckhard
B
Mentler
Matthias
M
Vogt
Wolfgang
W
D001798:Blood Proteins / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007263:Infusions, Parenteral; D007700:Kinetics; D010084:Oxidation-Reduction; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D012996:Solutions; D013054:Spectrophotometry, Atomic; D014168:Transferrin / Q000378:metabolism*; D014638:Vanadates / Q000737:chemistry / Q000378:metabolism*
D001798:Blood Proteins; D012709:Serum Albumin; D012996:Solutions; D014168:Transferrin; D014638:Vanadates