Detection and characterization of rotavirus G and P types from children participating in a rotavirus vaccine trial in Belém, Brazil.
This study sought the characterization of rotaviruses in a trial with a tetravalent rhesus-human rotavirus vaccine in Belém, Brazil in children who received three doses of vaccine or placebo in the 1st, 3rd and 5th months of life. Rotavirus electropherotypes, subgroups, G serotypes, G, [P] and [P], G genotypes were determined in 93.3%, 95.9%, 93.3%, 73.3%, 95.5% and 92.2% of isolates, respectively. Serotypes G1, G2 and G4 were detected in 58.9%, 30% and 4.4% of the cases, respectively. Rotavirus genotype G5 was detected for the first time in Northern region in 4.4% of the infections. Rotavirus genotypes P[8], P[4], P[6] and P[8 + 6] were detected in 54.5%, 26.7%, 12.2%, and 2.2% of the cases, respectively. The predominant genotypes were P[8], G1 and P[4], G2 with 53% and 26.6% of the infections, respectively. Unusual strains accounted for 20.5% including P[4], G1, P[6], G1, P[6], G4, P[6], G5, P[8], G2, P[8], G5. Mixed infections involving P[8 + 6], G2 and P[8 + 6], G1 were also noted. The neonatal P[6] strains associated with diarrhea were detected among children aged 9-24 months. To our knowledge, this study represents the first in Brazil to analyse, on molecular basis, rotavirus genotypes from children participating in a rotavirus vaccine trial. These results are of potential importance regarding future rotavirus vaccination strategies in Brazil.
Mascarenhas
J D P
JD
Instituto Evandro Chagas, Fundação Nacional de Saúde, 66090-000 Belém, PA, Brasil. joanamascarenhas@iec.pa.gov.br
Linhares
A C
AC
Gabbay
Y B
YB
Leite
J P G
JP
D001938:Brazil; D002648:Child; D002675:Child, Preschool; D004311:Double-Blind Method; D005759:Gastroenteritis / Q000821:virology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D011446:Prospective Studies; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D022243:Rotavirus Vaccines / Q000627:therapeutic use; D012703:Serotyping
D022243:Rotavirus Vaccines