Differential distribution of the fibres of the collagenous and elastic systems and of glycosaminoglycans in the rat pubic joint.
The pubic joint of male and female rats was studied at the light- and electron microscopical levels using methods that selectively disclose the extracellular matrix fibres and glycosaminoglycans. The interpubic tissue showed no difference between sexes (including pregnant and intrapartum females). The medial ends of the pubic bones were covered by articular caps of hyaline cartilage that blended in the midline. The whole articular cartilage was covered dorsally and ventrally (as well as craneally and caudally) by a typical perichondrium. The differential distribution of the fibres of the collagenous and elastic systems in the pubic joint agreed with the results reported in the literature for other rat cartilages. Collagen fibres, composed mainly of type-I collagen, were localised to the fibrous perichondrium and bone. Type-II collagen was localised to the central nucleus of hyaline cartilage, whereas reticulin fibres (rich in type-III collagen) were found in the adventitial loose connective tissue adherent to the most superficial layer of the perichondrium. The central nucleus of hyaline cartilage possessed the two types of elastic-related fibres: elaunin fibres were localised mainly to the chondrogenic layer of the perichondrium, whereas oxytalan fibres were found in the matrix that surrounded the chondrocytes. The bulk of the glycosaminoglycans present in the pubic joint cartilage corresponded to hyaluronic acid and chondroitin sulphate. The propriety of classification of the rat pubic joint as a true synchondrosis (instead of symphysis), and the fact that the unaltered pelvis of the rat seems to be adequate for normal parturition, are discussed.
Ortega
H H
HH
Department of Human Physiology, Faculty of Biochemistry and Biological Sciences, UNL, Santa Fe, Argentina.
Joazeiro
P P
PP
Muñoz-de-Toro
M M
MM
Luque
E H
EH
Montes
G S
GS
D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism* / Q000648:ultrastructure; D024042:Collagen Type I / Q000378:metabolism / Q000648:ultrastructure; D024043:Collagen Type II / Q000378:metabolism / Q000648:ultrastructure; D024061:Collagen Type III / Q000378:metabolism / Q000648:ultrastructure; D003285:Contractile Proteins / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism* / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D008297:Male; D011247:Pregnancy; D011631:Pubic Symphysis / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar / Q000378:metabolism*; D012737:Sex Factors
D024042:Collagen Type I; D024043:Collagen Type II; D024061:Collagen Type III; D003285:Contractile Proteins; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; C049028:elaunin; C437744:oxytalan