L-Lactate transport into rat heart mitochondria and reconstruction of the L-lactate/pyruvate shuttle.
In vitro reconstruction of the L-lactate/pyruvate shuttle has been performed, which allows NADH oxidation outside rat heart mitochondria. Such a shuttle occurs due to the combined action of the novel mitochondrial L-lactate/pyruvate antiporter, which differs from the monocarboxylate carrier, and the mitochondrial L-lactate dehydrogenase. The rate of L-lactate/pyruvate antiport proved to regulate the shuttle in vitro.
Valenti
Daniela
D
Centro di Studio sui Mitocondri e Metabolismo Energetico, CNR, Via Amendola 165/A, 70126, Bari, Italy.
de Bari
Lidia
L
Atlante
Anna
A
Passarella
Salvatore
S
D000818:Animals; D017920:Antiporters / Q000737:chemistry*; D001692:Biological Transport; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism*; D008027:Light; D008928:Mitochondria / Q000378:metabolism*; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism*; D051381:Rats; D013050:Spectrometry, Fluorescence; D013386:Succinates / Q000378:metabolism; D027981:Symporters / Q000378:metabolism; D000099233:Monocarboxylate Transport Protein 1
D017920:Antiporters; D007770:L-Lactate Dehydrogenase; D007773:Lactates; D019344:Lactic Acid; D027501:Monocarboxylic Acid Transporters; D010100:Oxygen; D011773:Pyruvates; D013386:Succinates; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1; C017625:2-phenylsuccinate