Effect of IL-2 and IL-6 on parameters related to metastatic activity in a murine melanoma.
Previous results have shown that the metastatic colonization with B16F10 melanoma in vivo increased after in vitro treatment of the cells with IL-2 or IL-6. To further investigate the mechanisms underlying this effect, we have studied adhesion, invasion, and proliferation properties of B16 melanoma, using two sublines with different metastatic ability. Adhesion of tumor cells to Matrigel coats increased using IL-6, which also induced upregulation of VLA-4 expression in both sublines. Unexpectedly, invasion through Matrigel filters was almost completely inhibited by IL-6 and decreased in the presence of IL-2. Cell growth was not affected by these interleukins; however, IL-6 could partially overcome the proliferation blockade induced by stress conditions. Taken together, these results suggest that upregulation of adhesion properties and/or the protective effect induced by IL-6 could account for the enhancement of metastasis exerted by this interleukin.
García de Galdeano
A
A
Departamento de Biología Celular y Ciencias Morfológicas, Universidad del País Vasco, Leioa, España. gcpgasaa@lg.ehu.es
Cruz-Conde
J C
JC
Boyano
M D
MD
García-Vázquez
M D
MD
Cañavate
M L
ML
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000378:metabolism; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D039041:Integrin alpha4beta1; D016023:Integrins / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007376:Interleukin-2 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D007797:Laminin / Q000378:metabolism; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000378:metabolism / Q000556:secondary*; D009361:Neoplasm Invasiveness / Q000473:pathology / Q000503:physiopathology; D009362:Neoplasm Metastasis / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D011509:Proteoglycans / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism; D013234:Stem Cells / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D004338:Drug Combinations; D039041:Integrin alpha4beta1; D016023:Integrins; D007376:Interleukin-2; D015850:Interleukin-6; D007797:Laminin; D011509:Proteoglycans; D015375:Receptors, Interleukin-2; D016201:Receptors, Lymphocyte Homing; C059485:matrigel; D018799:Intercellular Adhesion Molecule-1; D003094:Collagen