title: Use of 13C-labeled glucose for measuring exogenous glucose oxidation in mice.
abstract: The author modified a respiratory gas analyzer to analyze the respiratory 13CO2 of 12 small laboratory animals all at once. To investigate the practical use of this system, mice were orally (OR) or intravenously (i.v.) given glucose solutions containing three different amounts of 13C-labeled glucose. Expired 13CO2 derived from exogenous glucose was detected within 10 minutes after administration in OR mice, but about 30 minutes in i.v. mice. The height of the peak of 13CO2 expiration was correlated with the administered 13C-glucose mass.
authors: Department of Food and Nutrition, School of Life Studies, Sugiyama Jogakuen University, Nagoya, Japan. kengo@food.sugiyama-u.ac.jp
authors: Kengo
authors: K
authors: Ishihara
authors: Shinichi
authors: S
authors: Oyaizu
authors: Wataru
authors: W
authors: Mizunoya
authors: Yoshiko
authors: Y
authors: Fukuchi
authors: Kyoden
authors: K
authors: Yasumoto
authors: Tohru
authors: T
authors: Fushiki
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D005947:Glucose / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction
chemical_list: D002247:Carbon Isotopes; D005947:Glucose