Within- and between-strain genetic variability in White Leghorn population detected through RAPD markers.
1. Five White Leghorn (WLH) strains, namely G, H, I, J and C, differing in selection history, were screened for randomly amplified polymorphic DNA (RAPD) polymorphism using 50 random decamer primers. 2. Only 12 primers detected polymorphism between the strains. Out of 96 bands scored using these primers, only 21 (21.9%) were found to be polymorphic. 3. Strains differed considerably for within-population genetic similarity, estimated either by band sharing (WS=0.836 to 0.968) or band frequency (WF=0.820 to 0.969). 4. Between-strain genetic similarity estimates, based on band sharing (BS) as well as on band frequency (BF) ranged from 0.756 to 0.958 and from 0.830 to 0.996, respectively. Strains C and H were most closely related to each other, while J appeared to be more distant from other strains. 5. The between-strain genetic distance estimates also revealed similar trends. 6. A phylogenetic tree, derived using Nei's coefficient of similarity revealed similar relationships between the WLH strains.
Singh
R V
RV
Central Avian Research Institute, Izatnagar, UP, India.
Sharma
D
D
D000818:Animals; D002645:Chickens / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012641:Selection, Genetic