Optimization of a human papillomavirus-specific enzyme-linked immunosorbent assay.
A strategy was developed for the control, standardization, and critical evaluation of an enzyme-linked immunosorbent assay (ELISA) for the detection of human papillomavirus-specific immunoglobulin G in human sera. Control human sera, polyclonal animal sera, and monoclonal antibodies were used to establish optimal assay parameters, including antigen coating, serum dilutions, and criteria for daily reproducibility, monitoring, and rejection of assays. Three evaluation techniques were used in parallel to define an optimal cutoff absorbance value that yields greater than 93% sensitivity and 98.5% specificity in the assay's ability to discriminate positive and negative control sera. This strategy provides an optimal method by which to determine cutoff absorbance values for ELISA.
Karem
Kevin L
KL
Viral Exanthems and Herpesvirus Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. kkarem@cdc.gov
Poon
Alysia C
AC
Bierl
Cynthia
C
Nisenbaum
Rosane
R
Unger
Elizabeth
E
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D036022:Capsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology; D027383:Papillomaviridae / Q000276:immunology*; D030361:Papillomavirus Infections / Q000097:blood / Q000276:immunology / Q000821:virology*; D011817:Rabbits; D015203:Reproducibility of Results; D014412:Tumor Virus Infections / Q000097:blood / Q000276:immunology / Q000821:virology*
D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; C094776:L1 protein, Human papillomavirus type 11; D009856:Oncogene Proteins, Viral; C063317:L1 protein, Human papillomavirus type 16