Phylogenetic analysis of a human isolate from the 2000 Israel West Nile virus epidemic.
Specimens from a patient of the 2000 Israel West Nile virus epidemic were analyzed by reverse transcription-polymerase chain reaction. Products corresponding to E, NS3, and NS5 sequences were amplified from cerebellar but not from cortical samples. Phylogenetic analyses indicated a closer relationship of this isolate to 1996 Romanian and 1999 Russian than to 1998-99 Israeli or 1999 New York isolates.
Briese
Thomas
T
Emerging Diseases Laboratory, Dept. of Neurology, Microbiology and Molecular Genetics, 3107 Gillespie Neuroscience Building, University of California at Irvine, Irvine, CA 92697-4292, USA. tbriese@uci.edu
Rambaut
Andrew
A
Pathmajeyan
Melissa
M
Bishara
Jihad
J
Weinberger
Miriam
M
Pitlik
Silvio
S
Lipkin
W Ian
WI
D000368:Aged; D004196:Disease Outbreaks; D017809:Fatal Outcome; D005260:Female; D006801:Humans; D007557:Israel; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014901:West Nile Fever / Q000821:virology*; D014902:West Nile virus / Q000235:genetics* / Q000302:isolation & purification*