Cooperative action of alpha-glucanotransferase and maltogenic amylase for an improved process of isomaltooligosaccharide (IMO) production.
Maltogenic amylase and alpha-glucanotransferase (alpha-GTase) were employed in an effort to develop an efficient process for the production of isomaltooligosaccharides (IMOs). Bacillus stearothermophilus maltogenic amylase (BSMA) and alpha-GTase from Thermotoga maritima were overexpressed in Escherichia coli using overexpression vectors. An IMO mixture containing 58% of various IMOs was produced from liquefied corn syrup by the hydrolyzing and transglycosylation activities of BSMA alone. When BSMA and alpha-GTase were reacted simultaneously, the IMO content increased to 68% and contained relatively larger IMOs compared with the products obtained by the reaction without alpha-GTase. Time course analysis of the IMO production suggested that BSMA hydrolyzed maltopentaose and maltohexaose most favorably into maltose and maltotriose and transferred the resulting molecules simultaneously to acceptor molecules to form IMOs. alpha-GTase transferred donor sugar molecules to the hydrolysis products such as maltose and maltotriose to form maltopentaose, which was then rehydrolyzed by BSMA as a favorable substrate.
Lee
Hyun-Soo
HS
Samyang Genex Biotech Research Institute, 63-2 Hwaam-Dong, Yusung-Gu, Taejeon 305-348, Korea.
Auh
Joong-Hyuck
JH
Yoon
Hyun-Geun
HG
Kim
Myo-Jeong
MJ
Park
Jin-Hee
JH
Hong
Seung-Suh
SS
Kang
Min-Hyung
MH
Kim
Tae-Jip
TJ
Moon
Tae-Wha
TW
Kim
Jung-Wan
JW
Park
Kwan-Hwa
KH
D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006004:Glycogen Debranching Enzyme System / Q000235:genetics / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D007534:Isomaltose / Q000096:biosynthesis; D009844:Oligosaccharides / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D014312:Trisaccharides / Q000096:biosynthesis
D006004:Glycogen Debranching Enzyme System; D009844:Oligosaccharides; D011994:Recombinant Proteins; D014312:Trisaccharides; C045441:isomaltotriose; C047512:isomaltotetraose; C047511:isomaltopentaose; C024046:isomaltohexose; D007534:Isomaltose; C020569:4 alpha-glucanotransferase; D006026:Glycoside Hydrolases; C120909:glucan 1,4-alpha-maltohydrolase