Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay--assay validation for human milk.
In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.
Sugawara
Yukio
Y
Cosmo Oil Co Ltd, Tokyo, Japan. yukio_sugawara@cosmo-oil.co.jp
Saito
Koichi
K
Ogawa
Masahiko
M
Kobayashi
Susumu
S
Shan
Guomin
G
Sanborn
James R
JR
Hammock
Bruce D
BD
Nakazawa
Hiroyuki
H
Matsuki
Yasuhiko
Y
D000328:Adult; D001572:Benzofurans / Q000032:analysis / Q000633:toxicity*; D000072338:Dibenzofurans, Polychlorinated; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005544:Forecasting; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D008962:Models, Theoretical; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis / Q000633:toxicity*; D012016:Reference Values; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*
D001572:Benzofurans; D000072338:Dibenzofurans, Polychlorinated; D000072317:Polychlorinated Dibenzodioxins; D012989:Soil Pollutants