Cereal supplementation modified the fibrolytic activity but not the structure of the cellulolytic bacterial community associated with rumen solid digesta.
4 ruminally cannulated cows were fed a forage diet (93% hay + 7% straw) and a mixed diet (33 % hay + 7% straw + 40% barley) in a 2 x 2 crossover experimental design. In sacco degradation of forage, fibrolytic activities (polysaccharidases and glycosidases) of the solid-associated bacteria (SAB), and distribution of the 3 main cellulolytic bacterial species (Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens) were determined for both diets. Barley supplementation decreased the hay degradation rate and mainly the polysaccharidase activities of the SAB (30% on average). The sum of rRNA of the 3 cellulolytic bacterial species represented on average 17% of the total bacterial signal and R. albus was the dominant cellulolytic bacterial species of the 3 studied. Barley supplementation did not modify the proportion of the 3 cellulolytic bacteria attached to plant particles. The negative effect of barley on the ruminal hay degradation rate is due to a decrease in fibrolytic activity of the SAB, and not to a modification of the balance of the three cellulolytic bacterial species examined.
Martin
C
C
Unité de Recherche sur les Herbivores, INRA, Centre de Clermont-Theix, Saint-Genès-Champanelle, France. cecile@clermont.inra.fr
Millet
L
L
Fonty
G
G
Michalet-Doreau
B
B
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001421:Bacteria, Anaerobic / Q000201:enzymology* / Q000235:genetics; D001422:Bacterial Adhesion / Q000502:physiology; D002404:Catheterization; D002417:Cattle / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D018592:Cross-Over Studies; D004043:Dietary Fiber / Q000378:metabolism*; D019587:Dietary Supplements; D002523:Edible Grain / Q000378:metabolism*; D005260:Female; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000378:metabolism; D001467:Hordeum; D015336:Molecular Probe Techniques / Q000662:veterinary; D015347:RNA Probes; D011897:Random Allocation; D012417:Rumen / Q000378:metabolism / Q000382:microbiology*; D013045:Species Specificity
D004043:Dietary Fiber; D015347:RNA Probes; D002482:Cellulose; D006026:Glycoside Hydrolases