Organellar proteomics: the prizes and pitfalls of opening the nuclear envelope.
Proteomic studies have the potential to comprehensively define the composition of organelles but are limited by the organellar cross-contamination that arises during subcellular fractionation. Comparative proteomics of organellar subfractions can mitigate these problems, as demonstrated by a recent study involving the nuclear envelope.
Schirmer
Eric C
EC
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA. lgerace@scripps.edu
Gerace
Larry
L
D000818:Animals; D002458:Cell Fractionation; D008565:Membrane Proteins / Q000032:analysis; D009685:Nuclear Envelope / Q000737:chemistry*; D009687:Nuclear Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*
D008565:Membrane Proteins; D009687:Nuclear Proteins; D020543:Proteome