title: The effect of heating against Cryptosporidium oocysts.
abstract: The effect of heat treatment was examined against oocysts of Cryptosporidium parvum, Cryptosporidium muris and chicken Cryptosporidium sp. isolated in Japan. The oocysts of these species were exposed at 50, 55, 60 and 70 degrees C for 5, 15, 30 and 60 sec in water bath, respectively. To determine the infectivity of heated oocysts, the nice and chickens were inoculated with the treated oocysts and the oocyst output in the feces after inoculation was examined. In C. parvum and chicken Cryptosporidium sp., the oocysts were not detected from mice or chickens which were received oocysts heated at 55 degrees C for 30 sec, 60 degrees C for 15 sec and 70 degrees C for 5 sec. In C. muris, the oocysts were not detected from mice which were received oocysts heated at 55 degrees C for 15 sec, 60 degrees C for 15 sec and 70 degrees C for 5 sec. Consequently, it was clarified that the infectivity of Cryptosporidium oocysts to mice and chickens was lost by heating at 55 degrees C for 30 sec, 60 degrees C for 15 sec and 70 degrees C for 5 sec.
authors: Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan.
authors: Takashi
authors: T
authors: Fujino
authors: Toshihiro
authors: T
authors: Matsui
authors: Fumie
authors: F
authors: Kobayashi
authors: Kosuke
authors: K
authors: Haruki
authors: Yukiko
authors: Y
authors: Yoshino
authors: Junko
authors: J
authors: Kajima
authors: Moriyasu
authors: M
authors: Tsuji
mesh_terms: D000818:Animals; D002645:Chickens; D003457:Cryptosporidiosis / Q000662:veterinary*; D003458:Cryptosporidium / Q000254:growth & development*; D005243:Feces / Q000469:parasitology; D006358:Hot Temperature; D007564:Japan; D051379:Mice; D008813:Mice, Inbred ICR; D016513:Mice, SCID; D009865:Oocytes / Q000502:physiology*; D011201:Poultry Diseases / Q000469:parasitology*; D013047:Specific Pathogen-Free Organisms