Vasopressin immunoreactivity and release in the suprachiasmatic nucleus of wild-type and tau mutant Syrian hamsters.
Despite the prominent role of the Syrian hamster (Mesocricetus auratus) in studies of circadian rhythms, there are no data available on the temporal dynamics of the neuropeptide vasopressin (AVP), a major output system of the suprachiasmatic nucleus (SCN). We studied the hamster SCN-AVP system in vivo across the light period and in vitro using long-term organotypic SCN cultures. Additionally, we compared wild-type and tau mutant hamsters with an endogenous circadian period of approximately 24 h and approximately 20 h, respectively. The in vivo study revealed no differences in the number of SCN-AVP neurons between the two genotypes of hamsters studied at three time points across the light period of the circadian cycle. A significantly higher level of AVP-immunoreactivity, however, was found in the SCN of wild-type compared to tau mutant hamsters at the beginning and in the middle of the light period, but not at the end of the light period. SCN-AVP cell number and immunostaining decreased significantly across the light period in wild-type hamsters, but not in tau mutants. The in vitro study revealed a significantly higher rate of AVP release per 24 h from the tau mutant SCN compared to the wild-type SCN. Robust circadian oscillations in AVP release were not found in either type of hamster. These results may suggest that the SCN-AVP system of hamsters, irrespective of genotype, is relatively weak compared to other species. Moreover, the tau mutation seems to influence the SCN-AVP system by enhancing the rate of AVP release and by reducing AVP content and its daily fluctuation.
Van der Zee
Eddy A
EA
Zoological Laboratory, University of Groningen, P.O. Box 14, 9750 AA, Haren, The Netherlands. e.a.van.der.zee@biol.rug.nl
Oklejewicz
Malgorzata
M
Jansen
Koen
K
Daan
Serge
S
Gerkema
Menno P
MP
D000818:Animals; D001683:Biological Clocks / Q000235:genetics*; D047388:Casein Kinases; D002452:Cell Count; D002940:Circadian Rhythm / Q000235:genetics*; D006224:Cricetinae; D005838:Genotype; D007150:Immunohistochemistry; D008297:Male; D008647:Mesocricetus; D009154:Mutation / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009924:Organ Culture Techniques; D010775:Photic Stimulation; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000166:cytology / Q000378:metabolism*; D014667:Vasopressins / Q000378:metabolism*
D014667:Vasopressins; D011494:Protein Kinases; D047388:Casein Kinases