Determination of cannabinoids by gas chromatography-mass spectrometry and large-volume programmed-temperature vaporiser injection using 25 microl of biological fluid.
This paper presents a GC-MS confirmation method, based on large-volume programmed-temperature vaporisation (PTV) injection, for the determination of cannabinoids in plasma samples (or whole blood) with deuterium-labelled internal standards using only 25 microl of biological fluid. The analytes, Delta(9)-tetrahydrocannabinol (THC), 11-hydroxy-Delta(9)-tetrahydrocannabinol (11-OH-THC) and 11-nor-9-carboxy-Delta 9-tetrahydrocannabinol (THC-COOH), were enriched by means of solid-phase extraction cartridges containing octadecyl-bonded silica and were, subsequently, methylated. A 20 microl aliquot of an extract in hexane was injected into a PTV in solvent split mode. Method development and the results of the analyses of standard reference material and real samples are presented and discussed. This micro-method is precise and sensitive enough to assess relevant cannabinoid levels in human blood for forensic investigations as well as for clinical applications.
Teske
J
J
Institute of Legal Medicine, Leipzig University, Johannisallee 28, D-04103, Leipzig, Germany. teske@sonne.tachemie.uni-leipzig.de
Putzbach
K
K
Engewald
W
W
Müller
R K
RK
D000293:Adolescent; D002138:Calibration; D002186:Cannabinoids / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006358:Hot Temperature; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002186:Cannabinoids