title: Search for genes positively selected during primate evolution by 5'-end-sequence screening of cynomolgus monkey cDNAs.
abstract: It is possible to assess positive selection by using the ratio of K(a) (nonsynonymous substitutions per plausible nonsynonymous sites) to K(s) (synonymous substitutions per plausible synonymous sites). We have searched candidate genes positively selected during primate evolution by using 5'-end sequences of 21,302 clones derived from cynomolgus monkey (Macaca fascicularis) brain cDNA libraries. Among these candidates, 10 genes that had not been shown by previous studies to undergo positive selection exhibited a K(a)/K(s) ratio > 1. Of the 10 candidate genes we found, 5 were included in the mitochondrial respiratory enzyme complexes, suggesting that these nuclear-encoded genes coevolved with mitochondrial-encoded genes, which have high mutation rates. The products of other candidate genes consisted of a cell-surface protein, a member of the lipocalin family, a nuclear transcription factor, and hypothetical proteins.
authors: Division of Genetic Resources, National Institute of Infectious Diseases, Tokyo, Japan.osada@nih.go.jp
authors: Naoki
authors: N
authors: Osada
authors: Jun
authors: J
authors: Kusuda
authors: Makoto
authors: M
authors: Hirata
authors: Reiko
authors: R
authors: Tanuma
authors: Munetomo
authors: M
authors: Hida
authors: Sumio
authors: S
authors: Sugano
authors: Momoki
authors: M
authors: Hirai
authors: Katsuyuki
authors: K
authors: Hashimoto
mesh_terms: D000818:Animals; D003574:Cytochrome c Group / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D030561:Databases, Nucleic Acid; D003576:Electron Transport Complex IV / Q000235:genetics; D019143:Evolution, Molecular; D005796:Genes / Q000235:genetics; D008252:Macaca fascicularis / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011323:Primates / Q000235:genetics*; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D003574:Cytochrome c Group; D018076:DNA, Complementary; D003576:Electron Transport Complex IV