Bacterial colonization of intravenous catheters in young dogs suspected to have parvoviral enteritis.
OBJECTIVE
To determine the prevalence of bacterial colonization of IV catheters among young dogs suspected to have parvoviral enteritis, to identify the organisms responsible for catheter colonization, and to determine the antimicrobial susceptibility of organisms that were obtained.


METHODS
Case series.


METHODS
100 dogs.


METHODS
Catheters were aseptically removed when fluid therapy was discontinued, the catheter was replaced, or the dog died. The distal tip of the catheter was cut off, split open, and vortexed with sterile saline (0.9% NaCl) solution. The saline solution was plated on culture plates, which were then incubated and examined for bacterial growth every 24 hours for 72 hours. All bacteria cultured were identified, and antimicrobial susceptibility was determined.


RESULTS
Bacteria were isolated from 22 catheters. Most bacteria that were isolated were of gastrointestinal tract or environmental origin (Serratia odorifera, S. liquefaciens, S. marcescens, Acinobacter anitratus, Citrobacter freundii, Klebsiella pneumoniae, K. oxytoca, Escherichia coli, Enterobacter spp). Only 2 gram-positive organisms were isolated (Staphylococcus intermedius and Streptococcus spp). High percentages of organisms were resistant to penicillin, lincomycin, cloxacillin, erythromycin, and cephalexin. Percentages of organisms resistant to amikacin, enrofloxacin, chloramphenicol, potentiated sulfonamides, and amoxicillin-clavulanic acid were low.


CONCLUSIONS
Results suggest that IV catheters may be colonized with bacteria in 22% of young dogs suspected to have parvovirus infection.
Lobetti
Remo G
RG
Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
Joubert
Kenneth E
KE
Picard
Jackie
J
Carstens
Johan
J
Pretorius
Elize
E
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002408:Catheters, Indwelling / Q000009:adverse effects / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000382:microbiology* / Q000628:therapy / Q000821:virology; D004285:Dogs; D024901:Drug Resistance, Multiple, Bacterial; D004751:Enteritis / Q000628:therapy / Q000662:veterinary* / Q000821:virology; D005260:Female; D005440:Fluid Therapy / Q000662:veterinary; D008297:Male; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D010322:Parvoviridae Infections / Q000150:complications / Q000628:therapy / Q000662:veterinary*
D000900:Anti-Bacterial Agents