A single buffer that universally serves both restriction digestion and loading.
Restriction digestion is routinely performed in a buffer compatible with the restriction enzyme used. To load the samples on agarose gels for electrophoresis it is then necessary to add a loading buffer. A 10X loading buffer is often used, and consists of a dye to track the electrophoresis and a dense solution so that the digestion mixture sinks into the well. We describe a new buffer, which acts as a universal digestion buffer as well as a loading buffer. This avoids double handling of samples, which wastes both time and consumables. Importantly, the efficiency of digestion was found not to be significantly decreased in this new buffer.
Corbett
Alexandra J
AJ
Brady
Jamie L
JL
Lew
Andrew M
AM
D002021:Buffers; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D015183:Restriction Mapping / Q000379:methods*
D002021:Buffers; C047020:endodeoxyribonuclease ScaI; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific