Cloning and expression of a novel human galectin cDNA, predominantly expressed in placenta(1).
A novel human galectin cDNA (PPL13) was isolated by screening a human 18-week fetal brain library. The mRNA was predominantly expressed in placenta, while the expression of it was not or barely detectable in heart, brain, lung, liver, skeletal muscle, kidney, and pancreas by Northern blot. COS-7 cells transfected with cDNA encoding human PPL13 sequestered the protein in nuclei although it lacked any known nuclear localization signal. STS of Unigene Hs. 24236 placed the cDNA to human chromosome 19q13.2.
Yang
Quan-Sheng
QS
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai, PR China.
Ying
Kang
K
Yuan
Hong-Ling
HL
Chen
Jin-Zhong
JZ
Meng
Xian-Fang
XF
Wang
Zhao
Z
Xie
Yi
Y
Mao
Yu-Min
YM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D037161:Galectins; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism*; D011257:Pregnancy Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D014162:Transfection
D018076:DNA, Complementary; D037161:Galectins; C038195:LGALS13 protein, human; D011257:Pregnancy Proteins