title: Yersinia enterocolitica YopQ: strain-dependent cytosolic accumulation and post-translational secretion.
abstract: YopQ in Yersinia enterocolitica (YopK in Yersinia pseudotuberculosis) is a type III secreted protein required for virulence of yersiniae. In this study YopQ expression, secretion and nucleotide sequences of the corresponding yopQ gene from different yersinia strains were analysed. The cytosolic accumulation differed significantly among serotypes of Y. enterocolitica. These differences might be attributable to variations in the nucleotide sequence and their consequences on mRNA secondary structure. An mRNA signal hypothesis has been proposed for YopQ, predicting the coupling of translation and secretion via an mRNA signal. This hypothesis claims a strictly co-translational secretion of YopQ without its intracellular accumulation. The presence of YopQ in the cytosol, even with a closed secretion apparatus, is demonstrated. Moreover, post-translational secretion of YopQ could be demonstrated. These findings do not support the mRNA signal hypothesis for co-translational secretion.
authors: Max von Pettenkofer-Institut, Pettenkoferstr. 9a, D-80336 Munich, Germany1.
authors: Janja
authors: J
authors: Trček
authors: Max von Pettenkofer-Institut, Pettenkoferstr. 9a, D-80336 Munich, Germany1.
authors: Gottfried
authors: G
authors: Wilharm
authors: Max von Pettenkofer-Institut, Pettenkoferstr. 9a, D-80336 Munich, Germany1.
authors: Christoph A
authors: CA
authors: Jacobi
authors: Max von Pettenkofer-Institut, Pettenkoferstr. 9a, D-80336 Munich, Germany1.
authors: Jürgen
authors: J
authors: Heesemann
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D019286:Endopeptidase K / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D015008:Yersinia enterocolitica / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; C067570:YopQ protein, Yersinia enterocolitica; D019286:Endopeptidase K