Comparison of Fonsecaea pedrosoi sclerotic cells obtained in vivo and in vitro: ultrastructure and antigenicity.
The parasitic form of Fonsecaea pedrosoi from the hyperkeratotic layer of the skin was obtained from four patients with chromoblastomycosis. Primary cultures containing hyphae and conidia were successfully converted into sclerotic cells in the presence of 800 microM propranolol and low pH as described before. The morphology of sclerotic cells of F. pedrosoi obtained in vivo and in vitro was analyzed by light and electron microscopy. Their antigenicity was also compared by immunofluorescence microscopy and ELISA assays, using serum samples from untreated patients infected with F. pedrosoi. Due to the similarity of the sclerotic cells obtained in vivo and in vitro, the latter can be more adequately in studies of host-parasite interactions in chromoblastomycosis.
da Silva
Jorge P
JP
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Bloco G, CCS, RJ 21949-970, Brazil.
Alviano
Daniela S
DS
Alviano
Celuta S
CS
de Souza
Wanderley
W
Travassos
Luiz R
LR
Diniz
José A P
JA
Rozental
Sonia
S
D000328:Adult; D000368:Aged; D000946:Antigens, Fungal / Q000276:immunology; D002454:Cell Differentiation; D002862:Chromoblastomycosis / Q000382:microbiology* / Q000473:pathology; D003238:Connective Tissue / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D004817:Epidermis / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D003904:Mitosporic Fungi / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D011433:Propranolol / Q000494:pharmacology
D000946:Antigens, Fungal; D011433:Propranolol