Influence of organic solvents on papain kinetics.
Papain activity was studied in water-organic solvent mixtures using the fluorogenic substrate Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans. The increase of organic solvent (MeOH, EtOH, iPrOH, TFE, MeCN, (MeO)2Et and DMF) concentration in the mixture caused a substantial decrease the initial rate of papain-catalyzed hydrolysis. Moreover, the number of papain active sites decreased with the increase of DMF and MeOH concentration.
Szabelski
M
M
Faculty of Chemistry, University of Gdańsk, Poland.
Stachowiak
K
K
Wiczk
W
W
D000595:Amino Acid Sequence; D001665:Binding Sites; D004126:Dimethylformamide / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D010206:Papain / Q000737:chemistry*; D010452:Peptide Biosynthesis; D013050:Spectrometry, Fluorescence
D004126:Dimethylformamide; D010206:Papain