title: Conformational changes of colicin Ia channel-forming domain upon membrane binding: a solid-state NMR study.
abstract: Channel-forming colicins are bactericidal proteins that spontaneously insert into hydrophobic lipid bilayers. We have used magic-angle spinning solid-state nuclear magnetic resonance spectroscopy to examine the conformational differences between the water-soluble and the membrane-bound states of colicin Ia channel domain, and to study the effect of bound colicin on lipid bilayer structure and dynamics. We detected (13)C and (15)N isotropic chemical shift differences between the two forms of the protein, which indicate structural changes of the protein due to membrane binding. The Val C(alpha) signal, unambiguously assigned by double-quantum experiments, gave a 0.6 ppm downfield shift in the isotropic position and a 4 ppm reduction in the anisotropic chemical shift span after membrane binding. These suggest that the alpha-helices in the membrane-bound colicin adopt more ideal helical torsion angles as they spread onto the membrane. Colicin binding significantly reduced the lipid chain order, as manifested by (2)H quadrupolar couplings. These results are consistent with the model that colicin Ia channel domain forms an extended helical array at the membrane-water interface upon membrane binding.
authors: Department of Chemistry, Iowa State University, Ames, IA 50011, USA.
authors: Daniel
authors: D
authors: Huster
authors: Xiaolan
authors: X
authors: Yao
authors: Karen
authors: K
authors: Jakes
authors: Mei
authors: M
authors: Hong
mesh_terms: D000595:Amino Acid Sequence; D016880:Anisotropy; D002247:Carbon Isotopes; D002462:Cell Membrane / Q000737:chemistry*; D003087:Colicins / Q000737:chemistry*; D003903:Deuterium; D008051:Lipid Bilayers / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008565:Membrane Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D010759:Phosphorus Isotopes; D011485:Protein Binding; D011487:Protein Conformation
chemical_list: D002247:Carbon Isotopes; D003087:Colicins; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010759:Phosphorus Isotopes; D003903:Deuterium