Human erythroid cells produced ex vivo at large scale differentiate into red blood cells in vivo.
New sources of red blood cells (RBCs) would improve the transfusion capacity of blood centers. Our objective was to generate cells for transfusion by inducing a massive proliferation of hematopoietic stem and progenitor cells, followed by terminal erythroid differentiation. We describe here a procedure for amplifying hematopoietic stem cells (HSCs) from human cord blood (CB) by the sequential application of specific combinations of growth factors in a serum-free culture medium. The procedure allowed the ex vivo expansion of CD34+ progenitor and stem cells into a pure erythroid precursor population. When injected into nonobese diabetic, severe combined immunodeficient (NOD/SCID) mice, the erythroid cells were capable of proliferation and terminal differentiation into mature enucleated RBCs. The approach may eventually be useful in clinical transfusion applications.
Neildez-Nguyen
Thi My Anh
TM
Institut National de la Santé et de la Recherche Médicale U417, Hôpital Saint-Antoine, Paris, France.
Wajcman
Henri
H
Marden
Michael C
MC
Bensidhoum
Morad
M
Moncollin
Vincent
V
Giarratana
Marie-Catherine
MC
Kobari
Ladan
L
Thierry
Dominique
D
Douay
Luc
L
D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis; D002454:Cell Differentiation; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology*; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D051379:Mice; D016513:Mice, SCID; D010477:Perfusion; D013234:Stem Cells / Q000378:metabolism; D013997:Time Factors
D018952:Antigens, CD34; D016895:Culture Media, Serum-Free; D006454:Hemoglobins