[Expression of baculovirus recombinant HPV16L1 and L2 capsid proteins in insect cells].
BACKGROUND
To express capsid proteins of HPV16 in insect cells system.


METHODS
Recombinant baculovirus stocks were generated by cotransfection with baculovirus DNA and baculovirus recombinant transfer plasmids. The expression of aimed proteins in insect cells were confirmed by using SDS-PAGE and Western blot.


RESULTS
Two stable strains of recombinant baculovirus expressing HPV16L1 protein alone and co-expressing HPV16L1 plus L2 proteins were obtained. The L1 and L2 ORFs of HPV16 produced proteins of 57000 and 97000, respectively. The yield of L1 protein was about 25%-30% of the total proteins of the insect cell.


CONCLUSIONS
L1 and L2 proteins of HPV16 could be expressed with high efficiency in insect cells via recombinant baculoviruses.
Wei
L
L
Institute of Virology, Chinese Academy of Preventive Medicine,Beijing 100052, China.
Han
L
L
Ren
J
J
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002213:Capsid; D036022:Capsid Proteins; D005822:Genetic Vectors; D007313:Insecta / Q000821:virology; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis
D036022:Capsid Proteins; C063316:L2 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins; C063317:L1 protein, Human papillomavirus type 16