[Analysis and applications of a PrP antibody elicited by synthesized human PrP peptides].
BACKGROUND
To further study the immuno-reactivity and specificity of a PrP antibody elicited by synthesized human PrP peptides.


METHODS
Construction of a plasmid which expresses a N-terminus deleted human PrP protein and purification of this truncated protein. The prepared PrP antibody was tested for reaction with various prokaryotic expressed human PrP proteins,including the full-length protein,the N-terminus-and C-terminus-truncated proteins with Western blot. The antibody was also used in the immunofluorescence assays to identify the PrP proteins expressed from the cell lines transfected with human PrP genes. Moreover,the brain tissues from scrapie agents-infected hamsters and a CJD patient were extracted and the proteinase K-resistant PrP-res were analyzed with the prepared antibody.


RESULTS
The PrP-peptide elicited antibody could recognize both prokaryotic and eukaryotic expressed human PrP proteins, as well as the PrP-res proteins in the brain tissues both from the scrapie-infected hamsters and CJD patient.


CONCLUSIONS
The tested antibody possesses eligible immuno-reactivity and specificity and can be used for diagnosis of CJD in the clinical work.
Sun
X
X
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Dong
X
X
Zhang
B
B
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D016466:CHO Cells; D007562:Creutzfeldt-Jakob Syndrome / Q000175:diagnosis; D006224:Cricetinae; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis*; D018622:PrP 27-30 Protein / Q000276:immunology*; D011498:Protein Precursors / Q000032:analysis*; D012608:Scrapie / Q000175:diagnosis
D000906:Antibodies; D010446:Peptide Fragments; D011498:Protein Precursors; D018622:PrP 27-30 Protein