Differential anti-lipid peroxidative activity of melatonin.
Scavenging activities of melatonin, which is a pineal secretory product and functions in circadian biology, and its related compounds against reactive oxygen species such as superoxide anion radical, hydrogen peroxide, hydroxyl radical and singlet oxygen as well as organic peroxide radical (t-BuOO*) were evaluated chemically by using electron spin resonance-spin trap and chemiluminescence methods. Antioxidative activity of the compounds was estimated by IC50 value (microM), 50% inhibiting concentration of a compound against reactive oxygen species formed in each system, and the second-order rate constants (k2) for the reactions of the compounds and superoxide anion radical or hydroxyl radical. Because melatonin has exhibited the highest scavenging activity against t-BuOO*, the biochemical anti-lipid peroxide radical scavenging activities of melatonin were examined. We found that melatonin exhibits higher anti-lipid peroxidative activity in the rat brain microsomes than in the rat liver microsomal and liposomal systems, suggesting that melatonin may function as a treatment for reactive oxygen species-related diseases of the brain.
Kawanishi
Shinya
S
Department of Analytical and Bioinorganic Chemistry, Kyoto Pharmaceutical University, Japan.
Sakurai
Hiromu
H
D016166:Free Radical Scavengers; D007700:Kinetics; D015227:Lipid Peroxidation; D008550:Melatonin / Q000737:chemistry*; D013481:Superoxides; D020122:tert-Butylhydroperoxide
D016166:Free Radical Scavengers; D013481:Superoxides; D020122:tert-Butylhydroperoxide; D008550:Melatonin