High-efficiency fluorescence quenching of conjugated polymers by proteins.
The fluorescence of the water-soluble anionic conjugated polymer, poly[lithium 5-methoxy-2-(4-sulfobutoxy)-1,4-phenylenevinylene] (MBL-PPV), is quenched in dilute aqueous solution by cytochrome c, a small, naturally occurring electron-transfer protein. The large value obtained for the Stern-Volmer constant (K(sv) = 3.2 x 10(8) at pH 7.4, and approximately 10(9) in acidic solutions) is attributed to a combination of two factors: (1) facile ET between the luminescent semiconducting polymer and the protein and (2) the Columb attraction between the oppositely charged polyelectrolytes. This system shows significant potential for biosensor applications.
Fan
Chunhai
C
Institute for Polymers and Organic Solids, Department of Chemistry, University of California at Santa Barbara, 93106, USA..
Plaxco
Kevin W
KW
Heeger
Alan J
AJ
D015374:Biosensing Techniques; D003574:Cytochrome c Group / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D014753:Vinyl Compounds / Q000737:chemistry*
D003574:Cytochrome c Group; D009211:Myoglobin; D011108:Polymers; D014753:Vinyl Compounds; C460780:poly(lithium 5-methoxy-2-(4-sulfobutoxy)-1,4-phenylenevinylene); D009113:Muramidase