Molecular cloning and characterization of the mouse reticulon 3 cDNA.
cDNA of mouse reticulon 3 (mRTN3) was cloned. The cloned cDNA is 1745 bp long and contains an open reading frame of 237 amino acids for a 30 kDa protein. The gene was mapped at band B of chromosome 19 by FISH. Two altematively spliced transcripts, 3.4 and 2.3 kb, of mRTN3 were found by Northem blot analysis. Both transcripts were expressed in many tissues and embryos and the highest expression of the 3.4 kb-transcript was observed in the brain, especially in neurons. The expression of 30 kDa-mRTN3 protein was also greatest in the brain. Both N and C-termini of mRTN3 faced the cytosol, indicating that they may recruit other proteins to the endoplasmic reticulum.
Hamada
Nobuyuki
N
Department of Virology, Kurume University School of Medicine, Fukuoka, Japan.
Iwahashi
Jun
J
Suzuki
Kentaro
K
Ogi
Hidenao
H
Kashiwagi
Takahito
T
Hara
Koyu
K
Toyoda
Michiko
M
Yamada
Tatsuo
T
Toyoda
Tetsuya
T
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C482720:Rtn3 protein, mouse