The effects of intravenously administered methylxanthine preparations on the glycoconjugate composition of goblet cells in rabbit tracheal epithelium.
Effects of methylxanthine derivatives, which are inhibitors of phosphodiesterases I-IV used against bronchial asthma, on the composition of glycoconjugates in goblet cells were evaluated in tracheal epithelium of rabbits at 15 and 30 min after intravenous administration of aminophylline (Syntophyllin) and a mixture of etophylline and theophylline (Oxantil), respectively. Percentages of tracheal goblet cells containing neutral, total acidic, sulphated acidic, and sialylated acidic glycoconjugates were assessed using both conventional and lectin histochemistry. No significant changes were found in both experimental groups at 15 min after exposure. A significant decrease in percentage of alpha(2-3)-sialylated glycoconjugate-containing goblet cells occurred at 30 min after administration of Syntophyllin only. It is concluded that the mucus composition of tracheal goblet cells has been affected by the bronchospasmolytic drug Syntophyllin but not by the vasodilator drug Oxantil.
Vajner
Ludek
L
Institute of Histology and Embryology, 2nd Medical Faculty, Charles University Prague, Czech Republic. Ludek.Vajner@lfmotol.cuni.cz
Konrádová
Václava
V
Uhlík
Jirí
J
Zocová
Jarmila
J
D000628:Aminophylline / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D004338:Drug Combinations; D006001:Glycoconjugates / Q000378:metabolism*; D020397:Goblet Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007124:Immunoenzyme Techniques; D007275:Injections, Intravenous; D037102:Lectins / Q000378:metabolism; D008297:Male; D010726:Phosphodiesterase Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D011817:Rabbits; D013194:Staining and Labeling; D013806:Theophylline / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D004338:Drug Combinations; D006001:Glycoconjugates; D037102:Lectins; D010726:Phosphodiesterase Inhibitors; D000628:Aminophylline; D013806:Theophylline; C008749:etofylline