Circulating CD34+ cells to predict the adequate harvest of peripheral blood progenitor cells in platinum-based chemotherapy.
OBJECTIVE
The aim of this study was to clarify the appropriate timing for peripheral blood progenitor cells (PBPC) harvesting after platinum-based mobilization chemotherapy by measurement of the circulating CD34+ cell concentrations.


METHODS
PBPC were collected for autotransplantation via a total of 68 leukaphereses in 16 patients with gynecological cancer. Circulating CD34+ cell concentrations were measured by CD34-side scatter parameter analysis.


RESULTS
Data could be fitted into a linear regression line described by the equation y = 0.33 + 4.14 x (x) (R2 = 0.256), where y = the number of harvested colony-forming unit granulocyte macrophage (CFU-GM (x10(5)/kg) and x = the percentage of circulating CD34+ cells and y = 1.747 + 44.53 x (x) (R2= 0.475), where y = the number of harvested CD34+ cells (x10(6)/kg) and x = the percentage of circulating CD34+ cells. Failure to mobilize sufficient CFU-GM numbers (>1 x 10(5)/kg) occurred in 29 of 31 leukaphereses when the percentage of circulating CD34+ cells was less than 0.10%.


CONCLUSIONS
Harvesting procedure may be avoided when circulating CD34+ cells showed less than 0.10% after platinum-based mobilization chemotherapy.
Kurata
H
H
Department of Obstetrics and Gynecology, Niigata University Faculty of Medicine, Japan. tounin@med.niigata-u.ac.jp
Takakuwa
K
K
Tsuneki
I
I
Aoki
Y
Y
Tanaka
K
K
D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D002452:Cell Count; D003114:Colony-Forming Units Assay; D005260:Female; D006098:Granulocytes; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007937:Leukapheresis; D016014:Linear Models; D008264:Macrophages; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000628:therapy; D017671:Platinum Compounds / Q000627:therapeutic use*; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology; D020858:Tissue and Organ Harvesting; D014182:Transplantation, Autologous
D018952:Antigens, CD34; D017671:Platinum Compounds