title: Purification, characterization, thermal, and high-pressure inactivation of pectin methylesterase from bananas (cv Cavendish).
abstract: Pectin methylesterase (PME) was extracted from bananas (cv Cavendish) and purified by affinity chromatography on a CNBr-Sepharose-PME inhibitor (PMEI) column. A single protein and PME activity peak was obtained. For banana PME, a biochemical characterization in terms of molar mass (MM), pI, and kinetic parameters was performed. In a second step, the thermal and high-pressure stability of the enzyme was studied. Isothermal inactivation of purified banana PME could be described by a first-order kinetic model in a temperature range of 65 degrees to 72.5 degrees C, whereas its isobaric-isothermal inactivation followed a fractional-conversion model. Banana PME was found to be more thermally stable compared with PMEs extracted from orange, tomato, and apple.
authors: Laboratory of Food Technology, Department of Food and Microbial Technology, Faculty of Agricultural and Applied Biological Sciences, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, B-3001 Heverlee (Leuven), Belgium.
authors: B
authors: B
authors: Ly Nguyen
authors: A
authors: A
authors: Van Loey
authors: D
authors: D
authors: Fachin
authors: I
authors: I
authors: Verlent
authors: Indrawati
authors: M
authors: M
authors: Hendrickx
authors: I M
authors: IM
authors: Hendrickx
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007700:Kinetics; D028521:Musa / Q000201:enzymology*; D011312:Pressure
chemical_list: D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase