Development, evaluation, and application of lateral-flow immunoassay (immunochromatography) for detection of rotavirus in bovine fecal samples.
A lateral-flow immunoassay (LFT) was developed to detect bovine rotavirus in fecal samples. Using samples (n = 74) from diarrheic calves, a comparison of the LFT with a commercial latex agglutination test (LAT) and transmission electron microscopy (EM) was conducted. When EM was used as the reference method, initial studies of 29 samples indicated 70 and 80% sensitivities of the LFT and LAT, respectively, with both being 100% specific. When the LAT was the reference test, the LFT was 75% sensitive and 91% specific. Additional specimens (n = 45) were tested by the LFT and LAT alone, and results were identical for both methods.
Al-Yousif
Yousif
Y
Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506, USA.
Anderson
Joe
J
Chard-Bergstrom
Cindy
C
Kapil
Sanjay
S
D000818:Animals; D000956:Antigens, Viral / Q000097:blood* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000821:virology*; D002845:Chromatography / Q000379:methods; D003967:Diarrhea / Q000276:immunology / Q000821:virology*; D005243:Feces / Q000821:virology; D007118:Immunoassay / Q000379:methods; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary* / Q000821:virology
D000956:Antigens, Viral