title: Electrochemical studies on reconstituted horseradish peroxidase modified carbon paste electrodes.
abstract: Horseradish peroxidase (HRP) is a heme protein that acts specifically on H(2)O(2) as the electron acceptor. Hemin (Ferriprotoporhyrin-IX) is the prosthetic group of the enzyme. A direct molecular wire to the redox center of the enzyme is expected to enhance the electrochemical response of the enzyme. Native HRP was immobilized onto the surface of glassy carbon (GC) matrix using a 16-atom spacer arm. We have also immobilized the redox center of the enzyme (hemin) through one of the propionate groups onto the surface of glassy carbon matrix using an 11-atom spacer arm with amino terminus. Apoperoxidase was isolated according to the Teale's method and was allowed to reconstitute with the hemin-bound matrix for enzyme reconstitution. The HRP paste and reconstituted-HRP (rec-HRP) paste electrodes were used to study the electrochemical response to substrate H(2)O(2) using electrochemical techniques like cyclic voltammetry (CV) and flow injection (FI) studies. Flow injection studies using HRP paste electrode showed a linearity from 25 to 200 microM H(2)O(2). The rec-HRP paste showed approximately 100 times increase in the electron transfer rates compared to native HRP paste, and substrate linearity from 25 to 100 microM was observed.
authors: Department of Biochemistry, School of Life Sciences, University of Hyderabad, 500 046, Hyderabad 500 046, India. ckmslrs@uohyd.ernet.in
authors: Shailly
authors: S
authors: Varma
mesh_terms: D002244:Carbon / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006735:Horseradish Peroxidase / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D002244:Carbon; D006735:Horseradish Peroxidase