Molecular epidemiology of subgroup C avian pneumoviruses isolated in the United States and comparison with subgroup a and B viruses.
The avian pneumovirus (APV) outbreak in the United States is concentrated in the north-central region, particularly in Minnesota, where more outbreaks in commercial turkeys occur in the spring (April to May) and autumn (October to December). Comparison of the nucleotide and amino acid sequences of nucleoprotein (N), phosphoprotein (P), matrix (M), fusion (F), and second matrix (M2) genes of 15 U.S. APV strains isolated between 1996 and 1999 revealed between 89 and 94% nucleotide sequence identity and 81 to 95% amino acid sequence identity. In contrast, genes from U.S. viruses had 41 to 77% nucleotide sequence identity and 52 to 78% predicted amino acid sequence identity with European subgroup A or B viruses, confirming that U.S. viruses belonged to a separate subgroup. Of the five proteins analyzed in U.S. viruses, P was the most variable (81% amino acid sequence identity) and N was the most conserved (95% amino acid sequence identity). Phylogenetic comparison of subgroups A, B, and C viruses indicated that A and B viruses were more closely related to each other than either A or B viruses were to C viruses.
Shin
Hyun-Jin
HJ
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA.
Cameron
Kjerstin T
KT
Jacobs
Janet A
JA
Turpin
Elizabeth A
EA
Halvorson
David A
DA
Goyal
Sagar M
SM
Nagaraja
Kakambi V
KV
Kumar
Mahesh C
MC
Lauer
Dale C
DC
Seal
Bruce S
BS
Njenga
M Kariuki
MK
D000595:Amino Acid Sequence; D000818:Animals; D003120:Colorado / Q000453:epidemiology; D017931:DNA Primers; D004196:Disease Outbreaks / Q000662:veterinary; D008910:Minnesota / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D018111:Pneumovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018186:Pneumovirus Infections / Q000453:epidemiology / Q000662:veterinary*; D011201:Poultry Diseases / Q000453:epidemiology / Q000821:virology*; D012621:Seasons; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014422:Turkeys; D014481:United States / Q000453:epidemiology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D017931:DNA Primers; D014764:Viral Proteins