The evaluation of the radioprotective effect of Triphala (an ayurvedic rejuvenating drug) in the mice exposed to gamma-radiation.
The effect of 0, 5, 6.25, 10, 12.5, 20, 25, 40, 50 and 80 mg/kg b. wt. of aqueous extract of triphala (an Ayurvedic herbal medicine) administrered intraperitoneally was studied on the radiation-induced mortality in mice exposed to 10 Gy of gamma-radiation. Treatment of mice with different doses of triphala consecutively for five days before irradiation delayed the onset of mortality and reduced the symptoms of radiation sickness when compared with the non-drug treated irradiated controls. The highest protection against GI (gastrointestinal) death was observed for 12.5 mg/kg triphala, where a highest number of survivors were reported up to 10 days post-irradiation. While 10 mg/kg triphala i.p. provided the best protection as evidenced by the highest number of survivors after 30 days post-irradiation in this group when compared with the other doses of triphala. Toxicity study showed that triphala was non-toxic up to a dose of 240 mg/kg, where no drug-induced mortality was observed. The LD50 dose i.p. of triphala was found to be 280 mg/kg b. wt. Our study demonstrates the ability of triphala as a good radioprotective agent and the optimum protective dose of triphala was 1/28 of its LD50 dose.
Jagetia
G C
GC
Department of Radiobiology, Kasturba Medical College, Manipal, India. gc.jagetia@kmc.edu
Baliga
M S
MS
Malagi
K J
KJ
Sethukumar Kamath
M
M
D000818:Animals; D029764:Combretaceae; D004305:Dose-Response Relationship, Drug; D019665:Euphorbiaceae; D005638:Fruit / Q000737:chemistry; D005720:Gamma Rays / Q000009:adverse effects*; D008297:Male; D008515:Medicine, Ayurvedic; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000401:mortality / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D015996:Survival Rate; D023382:Toxicity Tests, Acute / Q000379:methods
D010936:Plant Extracts; D011837:Radiation-Protective Agents