Expression of histidine decarboxylase and synthesis of histamine by human small cell lung carcinoma.
We recently found that human small cell lung carcinomas (SCLCs) express, in addition to other neuroendocrine markers, vesicular monoamine transporters. Our present results indicate that SCLCs are histaminergic. We detected the biosynthetic enzyme histidine decarboxylase by immunohistochemistry in paraffin sections of 12 biopsies of SCLC tumors. This finding was supported by immunoblotting and reverse transcription-polymerase chain reaction experiments using established SCLC cell lines, frozen and paraffin-embedded SCLC tumors. Moreover, we found histamine to be synthesized, stored, and released by cultured SCLC cells. Our novel observations may be useful for developing new diagnostic tools for this frequent and highly malignant tumor.
Graff
Lothar
L
Anatomisches Institut, Universität München, München, Germany.
Frungieri
Mónica
M
Zanner
Robert
R
Pohlinger
Agnes
A
Prinz
Christian
C
Gratzl
Manfred
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018288:Carcinoma, Small Cell / Q000378:metabolism / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006632:Histamine / Q000096:biosynthesis*; D006639:Histidine / Q000494:pharmacology; D006640:Histidine Decarboxylase / Q000096:biosynthesis*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D006639:Histidine; D006632:Histamine; D006640:Histidine Decarboxylase