title: Spontaneous and stimulated secretion of monocyte chemotactic protein-1 and macrophage migration inhibitory factor by peritoneal macrophages in women with and without endometriosis.
abstract: OBJECTIVE
  To assess spontaneous and stimulated secretion of monocyte chemotactic protein-1 (MCP-1) and macrophage migration inhibitory factor (MIF) by peritoneal macrophages in women with and without endometriosis.
  
  
  METHODS
  Macrophages were isolated from the peritoneal fluid and cultured for different periods of time (6, 20, and 44 hours) without any stimulation to determine spontaneous secretion of MCP-1 and MIF. Macrophages were also exposed to 1 microg/mL lipopolysaccharide for 6 hours to evaluate the stimulated secretion of these cytokines.
  
  
  METHODS
  Gynecology clinic and human reproduction research laboratory.
  
  
  METHODS
  Twelve fertile women and 11 women with endometriosis.
  
  
  METHODS
  Peritoneal fluid obtained at laparoscopy.
  
  
  METHODS
  Monocyte chemotactic protein-1 and MIF concentrations in the culture medium using ELISA.
  
  
  RESULTS
  Peritoneal macrophages of women with endometriosis demonstrated an increased capacity to secrete MCP-1 either spontaneously or after stimulation with lipopolysaccharide. They also showed a marked tendency for an increased secretion of MIF, but no statistically significant difference was found.
  
  
  CONCLUSIONS
  Monocyte chemotactic protein-1 and MIF production by peritoneal macrophages may contribute to paracrine and autocrine activation and to macrophage accumulation in the peritoneal cavity of women with endometriosis. These mechanisms may exacerbate peritoneal inflammation and favor the growth of endometrial implants.
authors: Département d'Obstétrique-Gynécologie, Faculté de Médecine, Université Laval, Québec, Canada. ali.akoum@crsfa.ulaval.ca
authors: Ali
authors: A
authors: Akoum
authors: Jihong
authors: J
authors: Kong
authors: Christine
authors: C
authors: Metz
authors: Marie Claude
authors: MC
authors: Beaumont
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004715:Endometriosis / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008263:Macrophage Migration-Inhibitory Factors / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*
chemical_list: D018932:Chemokine CCL2; D003470:Culture Media; D008070:Lipopolysaccharides; D008263:Macrophage Migration-Inhibitory Factors