title: Clonal structure of Shiga toxin (Stx)-producing and beta-D-glucuronidase-positive Escherichia coli O157:H7 strains isolated from outbreaks and sporadic cases in Hokkaido, Japan.
abstract: A total of 22 clonal phenotypic variants of Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 was isolated from six different locations in Hokkaido, Japan. These isolates were negative for sorbitol fermentation but positive for beta-D-glucuronidase (GUD+). They carried eaeA, EHEC-hlyA, pas and etpD genes like typical E. coli O157:H7 and, in addition, st1 and stx2 genes. However, they were shown to lack katP and espP genes that are present in typical STEC O157:H7. All these atypical GUD+ STEC O157:H7 isolates had very similar antimicrobial susceptibilities. Pulsed-field gel electrophoresis analysis with XbaI, SfiI, SwaI, SpeI and NotI indicated that they were identical or closely related to one another. From their phenotypic and genotypic features, these GUD+ STEC O157:H7 isolates may represent a distinct clone among STEC O157.
authors: Hokkaido Institute of Public Health, North-19, West-12, Kita-ku, Sapporo 060-0819, Japan.
authors: Hideki
authors: H
authors: Nagano
authors: Hokkaido Institute of Public Health, North-19, West-12, Kita-ku, Sapporo 060-0819, Japan.
authors: Toyo
authors: T
authors: Okui
authors: Hokkaido Institute of Public Health, North-19, West-12, Kita-ku, Sapporo 060-0819, Japan.
authors: Osamu
authors: O
authors: Fujiwara
authors: Hokkaido Institute of Public Health, North-19, West-12, Kita-ku, Sapporo 060-0819, Japan.
authors: Yasuhiro
authors: Y
authors: Uchiyama
authors: Hokkaido Institute of Public Health, North-19, West-12, Kita-ku, Sapporo 060-0819, Japan.
authors: Naoto
authors: N
authors: Tamate
authors: Hokkaido Institute of Public Health, North-19, West-12, Kita-ku, Sapporo 060-0819, Japan.
authors: Hiroyuki
authors: H
authors: Kumada
authors: Hokkaido Institute of Public Health, North-19, West-12, Kita-ku, Sapporo 060-0819, Japan.
authors: Y O
authors: YO
authors: Morimoto
authors: Hokkaido Institute of Public Health, North-19, West-12, Kita-ku, Sapporo 060-0819, Japan.
authors: Shoki
authors: S
authors: Yano
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D005260:Female; D005838:Genotype; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007564:Japan / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D022603:Shiga Toxins / Q000096:biosynthesis* / Q000235:genetics; D014774:Virulence
chemical_list: D004269:DNA, Bacterial; D022603:Shiga Toxins; D005966:Glucuronidase