A new syringopeptin produced by bean strains of Pseudomonas syringae pv. syringae.
Two strains (B728a and Y37) of the phytopathogenic bacterium Pseudomonas syringae pv. syringae isolated from bean (Phaseolus vulgaris) plants were shown to produce in culture both syringomycin, a lipodepsinonapeptide secreted by the majority of the strains of the bacterium, and a new form of syringopeptin, SP(22)Phv. The structure of the latter metabolite was elucidated by the combined use of mass spectrometry (MS), nuclear magnetic resonance (NMR) spectroscopy and chemical procedures. Comparative phytotoxic and antimicrobial assays showed that SP(22)Phv did not differ substantially from the previously characterized syringopeptin 22 (SP(22)) as far as toxicity to plants was concerned, but was less active in inhibiting the growth of the test fungi Rhodotorula pilimanae and Geotrichum candidum and of the Gram-positive bacterium Bacillus megaterium.
Grgurina
Ingeborg
I
Dipartimento di Scienze Biochimiche A. Rossi-Fanelli, Università di Roma La Sapienza, P.le A. Moro, 5, 00185 Rome, Italy. Ingeborg.Grgurina@uniroma1.it
Mariotti
Feliciana
F
Fogliano
Vincenzo
V
Gallo
Monica
M
Scaloni
Andrea
A
Iacobellis
Nicola S
NS
Lo Cantore
Pietro
P
Mannina
Luisa
L
van Axel Castelli
Valeria
V
Greco
Maria Luigia
ML
Graniti
Antonio
A
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000382:microbiology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D011549:Pseudomonas / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity; D014026:Nicotiana / Q000187:drug effects
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001427:Bacterial Toxins; D010456:Peptides, Cyclic; C464465:syringopeptin SP(22)Phv