A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells.
E2F-6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. To better elucidate the molecular mechanism of repression by E2F-6, we have purified the factor from cultured cells. E2F-6 is found in a multimeric protein complex that contains Mga and Max, and thus the complex can bind not only to the E2F-binding site but also to Myc- and Brachyury-binding sites. Moreover, the complex contains chromatin modifiers such as a novel histone methyltransferase that modifies lysine 9 of histone H3, HP1gamma, and Polycomb group (PcG) proteins. The E2F-6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells. These data suggest that these chromatin modifiers contribute to silencing of E2F- and Myc-responsive genes in quiescent cells.
Ogawa
Hidesato
H
Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA.
Ishiguro
Kei-Ichiro
K
Gaubatz
Stefan
S
Livingston
David M
DM
Nakatani
Yoshihiro
Y
D000595:Amino Acid Sequence; D001665:Binding Sites; D018797:Cell Cycle Proteins; D002843:Chromatin / Q000378:metabolism*; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D004268:DNA-Binding Proteins; D019281:Dimerization; D050684:E2F Transcription Factors; D050701:E2F6 Transcription Factor; D016193:G1 Phase; D020868:Gene Silencing; D006367:HeLa Cells; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; D006657:Histones / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D008745:Methylation; D008780:Methyltransferases / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D023302:Protein Footprinting; D011496:Protein Methyltransferases; D011506:Proteins; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D016192:Resting Phase, Cell Cycle; D050717:Retinoblastoma-Like Protein p130; D014157:Transcription Factors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
D018797:Cell Cycle Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050701:E2F6 Transcription Factor; C491744:E2F6 protein, human; D006657:Histones; D010750:Phosphoproteins; D011506:Proteins; D016271:Proto-Oncogene Proteins c-myc; C493837:RBL2 protein, human; D011994:Recombinant Proteins; D050717:Retinoblastoma-Like Protein p130; D014157:Transcription Factors; D000090266:Chromobox Protein Homolog 5; C455847:EHMT1 protein, human; D000076983:Histone Methyltransferases; D008780:Methyltransferases; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase