title: Orexin-1 receptor immunoreactivity in chemically identified target neurons in the rat hypothalamus.
abstract: Immunohistochemistry and Western blotting were used to determine the distribution of orexin receptors in the rat brain. Strong orexin receptor 1 (OX1R) immunoreactivity was detected in the hypothalamus including the arcuate, ventromedial, and tuberomammillary nuclei that are involved in feeding regulation. The neuropeptide Y- and proopiomelanocortin-containing neurons of the arcuate nucleus, which act to stimulate or to inhibit feeding, respectively, displayed intense OX1R immunoreactivity by double immunostaining. Western blotting analysis yielded a 50-kDa major band of OX1R.
authors: Department of Anatomy, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors: Ryusuke
authors: R
authors: Suzuki
authors: Hisa
authors: H
authors: Shimojima
authors: Hisayuki
authors: H
authors: Funahashi
authors: Shigeo
authors: S
authors: Nakajo
authors: Shuori
authors: S
authors: Yamada
authors: Jian-Lian
authors: JL
authors: Guan
authors: Shinobu
authors: S
authors: Tsurugano
authors: Kichiro
authors: K
authors: Uehara
authors: Yoichi
authors: Y
authors: Takeyama
authors: Sakae
authors: S
authors: Kikuyama
authors: Seiji
authors: S
authors: Shioda
mesh_terms: D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D001069:Appetite Regulation / Q000502:physiology*; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D007026:Hypothalamic Area, Lateral / Q000166:cytology / Q000378:metabolism; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism* / Q000503:physiopathology; D064446:Orexin Receptors; D000068797:Orexins; D011333:Pro-Opiomelanocortin / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide / Q000378:metabolism*; D014697:Ventromedial Hypothalamic Nucleus / Q000166:cytology / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C578627:Hcrtr1 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D009478:Neuropeptide Y; D009479:Neuropeptides; D064446:Orexin Receptors; D000068797:Orexins; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide; D011333:Pro-Opiomelanocortin