LC determination of aminoglutethimide enantiomers as dansyl and fluorescamine derivatives in tablet formulations.
Determination of dansyl (AG-DNS) and fluorescamine (AG-F) derivatives of rac-aminoglutethimide in tablet formulation by HPLC has been achieved on a cellulose tris-(3,5-dimethylphenyl carbamate), known as Chiralcel OD and OD-R under normal and reversed phase columns, respectively, using a fluorescence detector (lambda(ex), 360 nm; lambda(em), 530 nm for AG-DNS derivatives; lambda(ex), 395 nm, lambda(em), 495 nm for fluorescamine derivatives (AG-F)). The best results were obtained with mobile phase ethanol:cyclohexane:methanol (95:5:2 v/v/v) for AG-DNS derivatives and acetonitrile:0.5% ortho-phosphoric acid (85:15 v/v) containing 0.26 mM 1-hexanesulfonic acid sodium salt (HSA) for AG-F, respectively. The lower limit of detection (signal to noise ratio of 3:1) were found to be 20 ng ml(-1) for each enantiomer for AG-DNS and 20.5 ng ml(-1) for each diastreoisomer for AG-F.
Cesur
Nesrin
N
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Istanbul, 34452, Beyazit, Istanbul, Turkey.
Apak
T Idil
TI
Aboul-Enein
Hassan Y
HY
Ozkirimli
Sumru
S
D000616:Aminoglutethimide / Q000032:analysis*; D018931:Antineoplastic Agents, Hormonal / Q000032:analysis*; D002482:Cellulose; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000032:analysis*; D005450:Fluorescamine / Q000031:analogs & derivatives* / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D013607:Tablets
D018931:Antineoplastic Agents, Hormonal; D003619:Dansyl Compounds; D012996:Solutions; D013607:Tablets; D000616:Aminoglutethimide; D005450:Fluorescamine; D002482:Cellulose