title: The granulocyte colony-stimulating factor response after intrapulmonary and systemic bacterial challenges.
abstract: In contrast to many cytokines such as tumor necrosis factor (TNF)-alpha, we hypothesized that, after an intrapulmonary bacterial challenge, lung-derived granulocyte colony-stimulating factor (G-CSF) would subsequently enter the systemic circulation. BALB/c mice were given Escherichia coli or saline, either intratracheally or intravenously. Four hours after intratracheal E. coli administration, bronchoalveolar lavage fluid (BALF) and plasma G-CSF concentrations increased, compared with concentrations in phosphate-buffered saline-treated controls. Lung G-CSF messenger RNA (mRNA) increased to 586+/-229 copies G-CSF mRNA/ng ribosomal RNA (rRNA) from the values in control animals (<0.5 copies/ng rRNA). In contrast, G-CSF mRNA was not increased in the extrapulmonary tissues examined (liver, spleen, and kidney) in mice challenged with intratracheal E. coli (<1 copy/ng rRNA). Intravenous E. coli increased plasma G-CSF and TNF-alpha, but neither cytokine was detected in BALF. These data show that, after an intrapulmonary infection, both lung and circulating G-CSF increase and that the lung is the likely source.
authors: Department of Physiology, Section of Pulmonary Critical Care Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA.
authors: Lee J
authors: LJ
authors: Quinton
authors: Steve
authors: S
authors: Nelson
authors: Darren M
authors: DM
authors: Boé
authors: Ping
authors: P
authors: Zhang
authors: Qiu
authors: Q
authors: Zhong
authors: Jay K
authors: JK
authors: Kolls
authors: Gregory J
authors: GJ
authors: Bagby
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology / Q000502:physiology*; D016179:Granulocyte Colony-Stimulating Factor / Q000032:analysis* / Q000097:blood / Q000235:genetics; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D008168:Lung / Q000276:immunology* / Q000382:microbiology; D016676:Macrophages, Alveolar / Q000276:immunology; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis / Q000235:genetics; D013154:Spleen / Q000276:immunology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D012335:RNA, Ribosomal; D014409:Tumor Necrosis Factor-alpha; D016179:Granulocyte Colony-Stimulating Factor