title: Growth-associated protein 43 is up-regulated in the ganglion cells of the ischemic rat retina.
abstract: We investigated the expression and cellular localization of growth-associated protein (GAP)-43 in the rat retina following ischemia induced by transiently increased intraocular pressure. In the normal retina, GAP-43 immunoreactivity was restricted to profiles in the inner plexiform layer. Following ischemia and reperfusion, immunoreactivity appeared in ganglion cells. The cell density of labeled ganglion cells peaked three days post-lesion and then decreased at seven days. Quantitative evaluation by immunoblotting confirmed that GAP-43 expression increased at three days (to 190% of control levels) and then slightly decreased at seven days. Our findings suggest that some ganglion cells have the potential to regenerate through the up-regulation of GAP-43 in the ischemic rat retina.
authors: Department of Anatomy, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, Seoul 137-701, Korea.
authors: Won-Kyu
authors: WK
authors: Ju
authors: Jae-Sung
authors: JS
authors: Gwon
authors: Sung-Jin
authors: SJ
authors: Park
authors: Keun-Young
authors: KY
authors: Kim
authors: Jung-Il
authors: JI
authors: Moon
authors: Mun-Yong
authors: MY
authors: Lee
authors: Su-Ja
authors: SJ
authors: Oh
authors: Myung-Hoon
authors: MH
authors: Chun
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000503:physiopathology; D016923:Cell Death / Q000502:physiology*; D019922:GAP-43 Protein / Q000378:metabolism*; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D007429:Intraocular Pressure / Q000502:physiology; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology; D012164:Retinal Diseases / Q000378:metabolism* / Q000503:physiopathology; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism*; D013477:Superior Colliculi / Q000166:cytology / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology*; D014795:Visual Pathways / Q000166:cytology / Q000378:metabolism
chemical_list: D019922:GAP-43 Protein; D006735:Horseradish Peroxidase