title: Immunogenic protein variations of Clostridium chauvoei cellular antigens associated with the culture growth phase.
abstract: The immunoprotective capacity of four Clostridium chauvoei strains at different growth stages is reported. In all the strains tested, the cells coming from the stationary phase were those with the highest immunoprotective capacity and, depending on the strain, this protective capacity diminished or even disappeared in other phases. Protein profiles were similar in all the strains and few proteins were differentially expressed during growth as shown by SDS-PAGE. For strain 17, a local strain, a clear relationship was observed between the diminution of immunogenicity and the total loss of protective capacity of sonicated cells at late stationary phase.
authors: Area Microbiología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, 5700, Argentina.
authors: María Aída
authors: MA
authors: Mattar
authors: Teresa Inés
authors: TI
authors: Cortiñas
authors: Ana María Stefanini
authors: AM
authors: de Guzmán
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D001431:Bacteriological Techniques; D015153:Blotting, Western; D003013:Clostridium / Q000254:growth & development / Q000276:immunology*; D003015:Clostridium Infections / Q000276:immunology / Q000517:prevention & control; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D013010:Sonication
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007074:Immunoglobulin G