Neonatal hepatic steatosis by disruption of the adenosine kinase gene.
Neonatal hepatic steatosis (OMIM 228100) is a fatal condition of unknown etiology characterized by a pale and yellow liver and early postnatal mortality. In the present study, a deficit in adenosine-dependent metabolism is proposed as a causative factor. Physiologically, adenosine is efficiently metabolized to AMP by adenosine kinase (ADK), an enzyme highly expressed in liver. ADK not only ensures normal adenine nucleotide levels but also is essential for maintaining S-adenosylmethionine-dependent transmethylation processes, where adenosine, an obligatory product, has to be constantly removed. Homozygous Adk(-/-) mutants developed normally during embryogenesis. However, within 4 days after birth they displayed microvesicular hepatic steatosis and died within 14 days with fatty liver. Adenine nucleotides were decreased and S-adenosylhomocysteine, a potent inhibitor of transmethylation reactions, was increased in the mutant liver. Thus, a deficiency in adenosine metabolism is identified as a powerful contributor to the development of neonatal hepatic steatosis, providing a model for the rapid development of postnatally lethal fatty liver.
Boison
Detlev
D
Institute of Pharmacology and Toxicology, University of Zurich, CH-8057 Zurich, Switzerland. boison@pharma.unizh.ch
Scheurer
Louis
L
Zumsteg
Valérie
V
Rülicke
Thomas
T
Litynski
Piotr
P
Fowler
Brian
B
Brandner
Sebastian
S
Mohler
Hanns
H
D000227:Adenine Nucleotides / Q000378:metabolism; D000248:Adenosine Kinase / Q000235:genetics / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001049:Apnea / Q000201:enzymology / Q000235:genetics; D001831:Body Temperature; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D005260:Female; D018390:Gene Targeting; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008136:Longevity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012435:S-Adenosylhomocysteine / Q000378:metabolism
D000227:Adenine Nucleotides; D012435:S-Adenosylhomocysteine; D000248:Adenosine Kinase