Measurement of corticoids in the patients with clinical features indicative of mineralocorticoid excess.
BACKGROUND
A method for the measurement of five important serum and urinary corticoids on the syndrome of mineralcorticoid excess is reported. The methodology was combined gas chromatography-mass spectrometry (GC-MS) with selected ion-monitoring mode.


METHODS
After extraction with a solid-phase cartridge using an Oasis HLB copolymer, the residues were derivatized with a mixture of N-methyl-N-trimethylsilyltrifluoroacetamide/ammonium iodide/dithioerythritol (1000:4:5, v/w/w), and analyzed.


RESULTS
The linearity as the regression coefficients were >0.979 over a range of 1-500 ng/ml, and limit of detection ranged from 1 to 3 ng/ml while their analytical recoveries varied in the range of 75.7-94.9%. The overall precision (% CV) of the method were 3.2-7.2% and 3.6-6.3% for serum and urine, respectively. The accuracy expresses as % bias ranged from -4.1 to 6.4%. This assay was used on two patients with hypokalemic hypertension, and may be useful in ruling out mineralcorticoid excess (AME) type 1 or 2.


CONCLUSIONS
The present GC-MS technique may be useful to differentiate between the syndrome of AME and other hypertensive diseases with clinical features suggestive of mineralcorticoid excess because of the assay's reliablity and precision.
Choi
Man Ho
MH
Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 130-650, South Korea.
Hahm
Jong Ryeal
JR
Jung
Byung Hwa
BH
Chung
Bong Chul
BC
D000305:Adrenal Cortex Hormones / Q000097:blood* / Q000378:metabolism / Q000652:urine*; D000328:Adult; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D008659:Metabolic Diseases / Q000097:blood / Q000378:metabolism / Q000652:urine; D008901:Mineralocorticoids / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000305:Adrenal Cortex Hormones; D008901:Mineralocorticoids