title: Mixed urease/amphiphile LB films and their application for biosensor development.
abstract: The optimal conditions for the formation of a stable mixed urease/octadecylamine (ODA) film at the air-water interface are determined. This film is efficiently transferred onto the hydrophobized surface of a pH-ISFET, and the features of the urea enzymatic field-effect transistor (ENFET) are determined: detection limit 0.2 mM, response time 15 s, and dynamic range 0-20 mM.
authors: IFOS-LPC1, UMR CNRS 5621, Ecole Centrale de Lyon, 36 Avenue Guy de Collongue, BP 163, F69131 Ecully Cedex, France.
authors: Aidong
authors: A
authors: Zhang
authors: Yanxia
authors: Y
authors: Hou
authors: Nicole
authors: N
authors: Jaffrezic-Renault
authors: Jialiang
authors: J
authors: Wan
authors: Alexey
authors: A
authors: Soldatkin
authors: Jean-Marc
authors: JM
authors: Chovelon
mesh_terms: D000588:Amines / Q000737:chemistry*; D015374:Biosensing Techniques; D017550:Spectroscopy, Fourier Transform Infrared; D014510:Urease / Q000737:chemistry*
chemical_list: D000588:Amines; D014510:Urease; C009317:stearylamine