Effects of thioamide substitutions on the conformation and stability of alpha- and 3(10)-helices.
Thiopeptides, formed by replacing the amide oxygen atom with a sp(2) sulfur atom, are useful in protein engineering and drug design because they confer resistance to enzymatic degradation and are predicted to be more rigid. This report describes our free molecular dynamics simulations with explicit water and free energy calculations on the effects of thio substitutions on the conformation of alpha-helices, 3(10)-helices, and their relative stability. The most prominent structural effect of thio substitution is the increase in the hydrogen bond distance from 2.1 A for normal peptides to 2.7 A for thiopeptides. To accommodate for the longer C[double bond]S...H-N hydrogen bond, the (phi, psi) dihedral angles of the alpha-helix changed from (-66 degrees, -42 degrees) to (-68 degrees, -38 degrees), and the rise per turn increased from 5.5 to 6.3 A. For 3(10)-helices, the (phi, psi) dihedral angles (-60 degrees, -20 degrees) and rise per turn (6.0 A) changed to (-66 degrees, -12 degrees) and 6.8 A, respectively. In terms of relative stability, the most prominent change upon thio substitution is the decrease in the free energy difference, Delta A(alpha --> 3(10)), from 14 to 3.5 kcal/mol. Therefore, normal peptides are less likely to form 3(10)-helix than are thiopeptides. Component analysis of the Delta A(alpha --> 3(10)) reviews that the entropy advantage of the 3(10)-helix for both Ac-Ala(10)-NHMe and Act-Alat(10)-NHMe is attributed to the 3(10)-helix being more flexible than the alpha-helix. Interestingly, upon thio substitution, this differential flexibility is even more apparent because the alpha-helix conformation of Act-Alat(10)-NHMe becomes more rigid due to the bulkier sulfur atom.
Tran
Tran Trung
TT
Ludwig Institute for Cancer Research and Cooperative Research Centre for Cellular and Growth Factors, Melbourne, Victoria, 3050, Australia.
Zeng
Jun
J
Treutlein
Herbert
H
Burgess
Antony W
AW
D000409:Alanine / Q000031:analogs & derivatives; D001704:Biopolymers; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D012996:Solutions; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013816:Thermodynamics; D013854:Thioamides / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D001704:Biopolymers; D010455:Peptides; D011506:Proteins; D012996:Solutions; D013438:Sulfhydryl Compounds; D013854:Thioamides; C459452:polythioalanine; D014867:Water; C019529:polyalanine; D000409:Alanine