Effects of novel phenoxazine compounds, 2-amino-4, 4a-dihydro-4alpha, 7-dimethyl-3H-phenoxazine-3-one and 3-amino-1, 4alpha-dihydro-4alpha, 8-dimethyl-2H-phenoxazine-2-one on proliferation of phytohemagglutinin- or anti-human IgM-activated human peripheral blood mononuclear cells.
We examined the in vitro effects of 2-amino-4, 4alpha-dihydro-4alpha, 7-dimethyl-3H-phenoxazine-3-one(Phx 1)and 3-amino-1, 4alpha-dihydro-4a, 8-dimethyl-2H-phenoxazine-2-one (Phx 2) on the proliferation of phytohemagglutinin (PHA)- or anti-human IgM-activated human peripheral blood mononuclear cells (PBMC). Phx 1 and Phx 2 inhibited the incorporation of 3H-thymidine of PHA-activated PBMC by as much as 75% and 40%, respectively, at a concentration of 40 microM. The inhibition was dependent on the dose of Phx 1 and Phx 2. These results strongly suggest that Phx 1 and Phx 2 inhibit proliferation of T cells, because PHA specifically activates the T cells among PBMC. On the other hand, when PBMC were activated by anti-human IgM, which specifically stimulates B cells, the incorporation of 3H-thymidine was rather increased in the presence of 15.8 microM Phx 1 or Phx 2. However, at a higher concentration of Phx 1 or Phx 2 (50 microM), the incorporation of 3H-thymidine was increased by Phx 1, but was inhibited by Phx 2. These results suggest different effects of Phx 1 and Phx 2 on proliferation of human T and B cells.
Akazawa
Mami
M
Department of Geriatric Medicine, Tokyo Medical University, Japan.
Koshibu-Koizumi
Junko
J
Iwamoto
Toshihiko
T
Takasaki
Masaru
M
Nakamura
Michio
M
Tomoda
Akio
A
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008213:Lymphocyte Activation; D015394:Molecular Structure; D010078:Oxazines / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
C470370:3-amino-1,4a-dihydro-4a,8-dimethyl-2H-phenoxazine-2-one; D000888:Antibodies, Anti-Idiotypic; D010078:Oxazines; D010835:Phytohemagglutinins; C044203:anti-IgM; C039203:phenoxazine; D013936:Thymidine