title: Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome.
abstract: In signal transduction in eukaryotes, protein phosphorylation is a key event. To understand signaling processes, we must first acquire an inventory of phosphoproteins and their phosphorylation sites under different conditions. Because phosphorylation is a dynamic process, elucidation of signaling networks also requires quantitation of these phosphorylation events. In this article, we outline several methods for enrichment of phosphorylated proteins and peptides and discuss various options for their identification and quantitation with special emphasis on mass spectrometry-based techniques.
authors: Center for Experimental Bioinformatics, University of Southern Denmark, M, DK-5230, Odense, Denmark. mann@bmb.sdu.dk
authors: Matthias
authors: M
authors: Mann
authors: Shao En
authors: SE
authors: Ong
authors: Mads
authors: M
authors: Grønborg
authors: Hanno
authors: H
authors: Steen
authors: Ole N
authors: ON
authors: Jensen
authors: Akhilesh
authors: A
authors: Pandey
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002845:Chromatography / Q000379:methods; D005583:Fourier Analysis; D013058:Mass Spectrometry / Q000379:methods*; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D010766:Phosphorylation; D020543:Proteome / Q000737:chemistry*; D015398:Signal Transduction
chemical_list: D000906:Antibodies; D010750:Phosphoproteins; D020543:Proteome