Enzymatic synthesis of oligosaccharide containing Le(x) unit by using partially purified chicken serum.
Animal sera were screened for an alternative enzyme source of alpha1,3-fucosyltransferase, and the highest activity was observed in chicken serum. A partially purified enzyme fraction almost devoid of coexisting glycosidases was prepared from the chicken serum, and used for the fucosylation of LacNAc compounds. The enzyme reaction was efficient enough to allow the one-pot preparation of designed Le(x) compounds such as LNFP III.
Totani
Kazuhide
K
Science of Biological Resources, The United Graduate School of Agricultural Science, Gifu University, Japan.
Shimizu
Kaoru
K
Harada
Yoichiro
Y
Murata
Takeomi
T
Usui
Taichi
T
D000818:Animals; D002606:Charcoal; D002645:Chickens / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005647:Fucosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016256:Lewis X Antigen / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000138:chemical synthesis*
D016256:Lewis X Antigen; D009844:Oligosaccharides; D002606:Charcoal; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase