title: Complex polysaccharide inclusions in skeletal muscle adjacent to sarcomas in two dogs.
abstract: Inclusions of periodic acid-Schiff-positive, amylase resistant material were found within skeletal muscle fibers adjacent to an osteosarcoma in the proximal femur of an 8-year-old intact female Cocker Spaniel dog (dog No. 1) and adjacent to a synovial cell sarcoma of the stifle joint in a 7-year-old spayed female Bouvier des Flandres dog (dog No. 2). Inclusions were pale blue-gray with hematoxylin and eosin stain and formed irregular inclusions, replacing up to approximately 80% of the fiber diameter. Inclusions from dog No. 2 were of non-membrane-bound granular to filamentous material that occasionally formed discrete, elongate electron-dense masses. The features of these inclusions were similar to those of materials previously described as complex polysaccharide, polyglucosan bodies, amylopectin, and Lafora bodies. Evidence for a generalized metabolic disorder was not found in these two dogs, suggesting that storage of complex polysaccharide can occur as a relatively nonspecific response to metabolic alterations in skeletal muscle in a variety of conditions.
authors: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis 97331, USA.
authors: B A
authors: BA
authors: Valentine
authors: R J
authors: RJ
authors: Bildfell
authors: B J
authors: BJ
authors: Cooper
authors: U
authors: U
authors: Giger
authors: K A
authors: KA
authors: Fischer
mesh_terms: D000687:Amylopectin / Q000378:metabolism; D000818:Animals; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D005266:Femoral Neoplasms / Q000473:pathology / Q000662:veterinary*; D007592:Joint Diseases / Q000473:pathology / Q000662:veterinary; D008854:Microscopy, Electron / Q000662:veterinary; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D012516:Osteosarcoma / Q000473:pathology / Q000662:veterinary*; D011134:Polysaccharides / Q000378:metabolism*; D013584:Sarcoma, Synovial / Q000473:pathology / Q000662:veterinary*; D013264:Stifle
chemical_list: D011134:Polysaccharides; D000687:Amylopectin