Streptococcus pneumoniae polysaccharides conjugated to the outer membrane protein A from Klebsiella pneumoniae elicit protective antibodies.
Polysaccharides (PSs) derived from Streptococcus pneumoniae include more than 90 serotypes and differ greatly in their immunogenicity. In addition, immunization with PSs does not induce high affinity antibody production and no memory B-cells are generated. Coupling PSs to carrier proteins has been reported to induce B-cell maturation and to install a B-cell memory. As an alternative carrier protein, the outer membrane protein A (OmpA) derived from Klebsiella pneumoniae has been coupled to various PSs. We evaluated the immunogenicity of two PS conjugates, using PS derived from S. pneumoniae types 14 and 19. In this report, we show that anti-PS IgG responses are generated after the conjugation of PSs to P40. In addition, the humoral response generated is able to protect mice from a bacterial challenge. Our results indicate that P40 could be included in the development of new PS conjugate vaccines.
Libon
Christine
C
Centre d'Immunologie Pierre Fabre, 5 Avenue Napoléon III, St. Julien en Genevois, France. christine.libon@pierre-fabre.com
Haeuw
Jean François
JF
Crouzet
Françoise
F
Mugnier
Chantal
C
Bonnefoy
Jean Yves
JY
Beck
Alain
A
Corvaïa
Nathalie
N
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000145:classification; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis / Q000145:classification; D007711:Klebsiella pneumoniae; D051379:Mice; D008807:Mice, Inbred BALB C; D011008:Pneumococcal Infections / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology; D018074:Vaccines, Conjugate / Q000276:immunology*
D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D022242:Pneumococcal Vaccines; D011134:Polysaccharides; D018074:Vaccines, Conjugate; C078167:OMPA outer membrane proteins