title: Partially structured state of the functional VH domain of the mouse anti-ferritin antibody F11.
abstract: An antibody combining site generally involves the two variable domains, VH from the heavy and VL from the light chain. We expressed the individual VH domain of the mouse anti-human ferritin monoclonal antibody F11. The loss of affinity was not dramatic (K(a)=4.0x10(7) M(-1) versus 8.6x10(8) M(-1) for the parent antibody) and comparable to that previously observed for other VHs. However, the functional VH domain adopted a partially structured state with a significant amount of distorted secondary and compact yet greatly destabilized tertiary structures, as demonstrated by spectroscopic and calorimetric probes. These data provide the first description for a functional antibody domain that meets all the criteria of a partially structured state.
authors: Institute of Bio-Organic Chemistry, National Academy of Sciences of Belarus, 220141, Minsk, Byelorussia. martsev@iboch.bas-net.by
authors: Sergey P
authors: SP
authors: Martsev
authors: Anatoly P
authors: AP
authors: Dubnovitsky
authors: Oleg A
authors: OA
authors: Stremovsky
authors: Alexander A
authors: AA
authors: Chumanevich
authors: Yaroslav I
authors: YI
authors: Tsybovsky
authors: Zinaida I
authors: ZI
authors: Kravchuk
authors: Sergey M
authors: SM
authors: Deyev
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000915:Antibody Affinity; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D005293:Ferritins / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000276:immunology; D007142:Immunoglobulin gamma-Chains / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D011487:Protein Conformation; D017510:Protein Folding; D013050:Spectrometry, Fluorescence
chemical_list: D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region; D007142:Immunoglobulin gamma-Chains; D005293:Ferritins