Vitellogenin induction in Cyprinus carpio treated with 17beta-estradiol and 4-nonylphenol.
The aim of the present study was to demonstrate induction of vitellogenin in the common carp (Cyprinus carpio) as a biomarker for monitoring freshwater ecosystems. Sexually undifferentiated specimens of common carp were treated experimentally with 17beta-estradiol and increasing doses of 4-nonylphenol and levels of plasma Vtg were measured in order to: 1) validate an ELISA assay for plasma Vtg in the common carp using the polyclonal rabbit anti-salmon Vtg antibody AA1 (Biosense, Norway); 2) check the sensitivity of carp juveniles in producing Vtg in response to estrogen stimulation. The group treated with 17beta-estradiol showed high induction (156%) with respect to controls, also groups treated with 4-nonylphenol showed induction of Vtg. The group treated with 100 mg kg(-1) b.wt showed an induction of 61%. A statistically significant correlation was found between dose and response. This preliminary study demonstrate a response to the ELISA assay for Vtg in the common carp using rabbit anti-salmon antibody AA1. With further evidence the tested biomarker might be proposed for large scale monitoring of estrogenic effects caused by pollution in urban and industrial effluents.
Casini
Silvia
S
Department of Environmental Science, University of Siena, Italy. CasiniS@unisi.it
Fossi
M Cristina
MC
Mori
Gabriele
G
Bjornstad
Anne
A
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002347:Carps / Q000502:physiology*; D004781:Environmental Exposure; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000009:adverse effects / Q000494:pharmacology*; D010636:Phenols / Q000009:adverse effects / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D014819:Vitellogenins / Q000096:biosynthesis*
D015415:Biomarkers; D010636:Phenols; D014819:Vitellogenins; D004958:Estradiol; C041594:4-nonylphenol