title: Identification of a newly characterized HIV-1 BG intersubtype circulating recombinant form in Galicia, Spain, which exhibits a pseudotype-like virion structure.
abstract: We recently reported the finding of phylogenetically related HIV-1 BG intersubtype recombinant and G subtype nonrecombinant viruses circulating among injecting drug users in the region of Galicia in northwestern Spain. Here, we report the characterization of near full-length genome sequences of nine of these viruses (seven BG recombinant and two of nonrecombinant G subtype), obtained from epidemiologically unlinked individuals. Bootscan analysis reveals that six recombinant viruses share an identical mosaic structure, with two intersubtype breakpoints delimiting a B subtype segment comprising most of Env gp120 and the external portion of Env gp41, with the remaining portions of the genome being of subtype G, thus mimicking a pseudotype virion structure. The seventh BG recombinant virus exhibits breakpoints in env coincident with the other BG viruses but contains additional B subtype segments in gag and pol. In phylogenetic trees of complete genomes and of the B subtype segment of env, all seven BG viruses group in a monophyletic cluster. G subtype portions of the BG viruses group uniformly with the newly derived nonrecombinant G subtype viruses of Galicia in bootscan analysis, which points to the locally circulating G subtype strain as parental of the recombinants. These results allow us to define a new HIV-1 circulating recombinant form (CRF14_BG), the first reported to originate in Western Europe.
authors: Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain.
authors: Elena
authors: E
authors: Delgado
authors: Michael M
authors: MM
authors: Thomson
authors: María Luisa
authors: ML
authors: Villahermosa
authors: María
authors: M
authors: Sierra
authors: Antonio
authors: A
authors: Ocampo
authors: Celia
authors: C
authors: Miralles
authors: Raúl
authors: R
authors: Rodríguez-Pérez
authors: Julio
authors: J
authors: Diz-Aren
authors: Rafael
authors: R
authors: Ojea-de Castro
authors: Elena
authors: E
authors: Losada
authors: María Teresa
authors: MT
authors: Cuevas
authors: Elena
authors: E
authors: Vázquez-de Parga
authors: Rocío
authors: R
authors: Carmona
authors: Lucía
authors: L
authors: Pérez-Alvarez
authors: Leandro
authors: L
authors: Medrano
authors: Laureano
authors: L
authors: Cuevas
authors: José Antonio
authors: JA
authors: Taboada
authors: Rafael
authors: R
authors: Nájera
mesh_terms: D005260:Female; D016679:Genome, Viral; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D015700:HIV Envelope Protein gp41 / Q000235:genetics; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013030:Spain / Q000453:epidemiology; D015819:Substance Abuse, Intravenous / Q000150:complications; D014771:Virion
chemical_list: D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41