Oestrogen regulates neurofilament expression in a subset of anterior pituitary cells of the adult female rat.
It is the prevailing view that the neurohypophysis derives from neural crest while the pituitary's anterior lobe is of ectodermal origin. However, it has been recently suggested that anterior pituitary cells could have in part neuro-ectodermal origin, and thus should express specific neuronal markers. This issue was examined previously with conflicting results. The present study attempts to clarify the question of whether or not neuronal markers are expressed in the adenohypophysis. Using quantitative immunofluorescence, we have positively identified a subset of anterior pituitary cells, which express immunoreactivity for neuronal markers, including 68 kDa neurofilament (NF68). Interestingly, we noticed that the expression of NF68 is sexually dimorphic (i.e. neurofilament-positive cells are more abundant in sexually mature female rats). In addition, NF68 expression in female rats increases during ontogenic development and reaches a plateau level after puberty. Thereafter, it displays plastic changes along the oestrous cycle, with the maximum of neurofilament expression at oestrus and the minimum at proestrus. NF68 immunoreactivity was examined after ovariectomy, oestradiol replacement and treatment with an specific oestrogen receptor antagonist. Bilateral ovariectomy induced a significant reduction in the number of NF68-positive cells. This effect was completely prevented by treatment of ovariectomized rats with oestradiol. When intact female rats were treated with the anti-oestrogen tamoxifen, a drastic decrease in NF68 expression in anterior pituitary cells was observed. Furthermore, oestradiol administration in castrated male rats increased NF68 immunoreactivity. Double-immunolabelling experiments provided evidence that pituitary cells expressing neuronal traits correspond to subsets of lactotrophs, somatotrophs, thyrotrophs and gonadotrophs. It remains to be established if NF68 induction in the pituitary is due to direct and/or indirect effects of oestrogens. Also, the possible functional role of this subset of NF68-positive anterior pituitary cells in the female rat remains to be examined.
Fiordelisio
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T
Instituto de Fisiología Celular, Departamento de Biofísica, Universidad Nacional Autónoma de México, UNAM, Circuito Exterior, Ciudad Universitaria, México City, México.
Hernández-Cruz
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A
D000818:Animals; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000502:physiology*; D004971:Estrus / Q000502:physiology; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016900:Neurofilament Proteins / Q000378:metabolism*; D009919:Orchiectomy; D010052:Ovariectomy; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D012727:Sex Characteristics; D013629:Tamoxifen / Q000494:pharmacology
D004965:Estrogen Antagonists; D004967:Estrogens; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C066858:neurofilament protein NF 68; D013629:Tamoxifen; D004958:Estradiol