Paramagnetic resonance of biological metal centers.
The review deals with recent advances in magnetic resonance spectroscopy (hf EPR and NMR) of paramagnetic metal centers in biological macromolecules. In the first half of our chapter, we present an overview of recent technical developments in the NMR of paramagnetic bio-macromolecules. These are illustrated by a variety of examples deriving mainly from the spectroscopy of metalloproteins and their complexes. The second half focuses on recent developments in high-frequency EPR spectroscopy and the application of the technique to copper, iron, and manganese proteins. Special attention is given to the work on single crystals of copper proteins.
Ubbink
M
M
Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Worrall
J A R
JA
Canters
G W
GW
Groenen
E J J
EJ
Huber
M
M
D001665:Binding Sites; D001703:Biophysics / Q000379:methods; D003300:Copper / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D008962:Models, Theoretical
D003300:Copper