Spherical molecularly imprinted polymer particles: a promising tool for molecular recognition in capillary electrokinetic separations.
Spherical molecularly imprinted polymer particles obtained via precipitation polymerization, were introduced as a pseudostationary phase in capillary electrophoresis (CE) to study molecular recognition. Analyses were performed via a partial filling technique using (+)-ephedrine-imprinted microspheres (100-200 nm) which were polymerized from methacrylic acid and 1,1,1-Tris(hydroxymethyl)propanetrimethacrylate using acetonitrile as the solvent. The influence of pH and the modifier content on the separation was investigated. A 0.1% w/v suspension in an aqueous 10 mM phosphate buffer (pH 2.5 with 40% acetonitrile) was hydrodynamically injected into the CE system (80% of the effective capillary length) and led to full baseline separation of racemic ephedrine within 10 min.
De Boer
Theo
T
Department of Analytical Chemistry and Toxicology, University Centre for Pharmacy, Groningen, The Netherlands.
Mol
Roelof
R
De Zeeuw
Rokus A
RA
De Jong
Gerhardus J
GJ
Sherrington
David C
DC
Cormack
Peter A G
PA
Ensing
Kees
K
D018663:Adrenergic Agents / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods; D020374:Chromatography, Micellar Electrokinetic Capillary; D003432:Cross-Linking Reagents; D004809:Ephedrine / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008863:Microspheres; D013237:Stereoisomerism
D018663:Adrenergic Agents; D003432:Cross-Linking Reagents; D004809:Ephedrine