title: A hammerhead ribozyme substrate and reporter for in vitro kinetoplastid RNA editing.
abstract: Current in vitro assays for RNA editing in kinetoplastids directly examine the products generated by incubation of pre-mRNA substrate with guide RNA (gRNA) and mitochondrial (mt) extract. RNA editing substrates that are modeled on hammerhead ribozymes were designed with catalytic cores that contained or lacked additional uridylates (Us). They proved to be sensitive reporters of editing activity when used for in vitro assays. A deletion editing substrate that is based on A6 pre-mRNA had no ribozyme activity, but its incubation with gRNA and mt extract resulted in its deletion editing and production of a catalytically active ribozyme. Hammerhead ribozymes are thus sensitive tools to assay in vitro RNA editing.
authors: Department of Pathobiology, University of Washington, Seattle, 98195, USA.
authors: Bingbing
authors: B
authors: Wang
authors: Reza
authors: R
authors: Salavati
authors: Stefan
authors: S
authors: Heidmann
authors: Kenneth
authors: K
authors: Stuart
mesh_terms: D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D017393:RNA Editing; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000235:genetics*; D014542:Uridine Monophosphate / Q000737:chemistry
chemical_list: D016337:RNA, Catalytic; C427204:hammerhead ribozyme; D014542:Uridine Monophosphate