Effects of thyroid hormone on mRNAs of phosphoglycerate mutase subunits in rat muscle during development.
BACKGROUND
We previously showed that triiodothyronine (T3) stimulates muscle phosphoglycerate mutase (PGAM) activity and isozyme transition in rat skeletal and cardiac muscles.


METHODS
The effects of T3 on PGAM types B and M subunit expression in rat muscle during development are reported.


RESULTS
T3 administration during the first 21 days of rat life more than doubles type M PGAM mRNA levels, but produces minor effects on type B PGAM mRNA levels. The antihormone propylthiouracil (PTU) slightly decreases both type B and M mRNA levels, but this decrease is not statistically significant.


CONCLUSIONS
Thyroid hormone influences PGAM mRNA isozyme levels differently and increases type M mRNA.
González-Cinca
Núria
N
Unitat de Bioquimica, Departament de Ciéncies Fisiològiques I, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Facultat de Medicina, Universitat de Barcelona, Spain.
Gonzalo
Sergio
S
Ascaso
Carlos
C
Carreras
José
J
Climent
Fernando
F
D000818:Animals; D000831:Animals, Newborn; D013956:Antithyroid Agents / Q000494:pharmacology; D015152:Blotting, Northern; D003720:Densitometry; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006321:Heart / Q000187:drug effects / Q000254:growth & development*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development*; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D010736:Phosphoglycerate Mutase / Q000096:biosynthesis* / Q000235:genetics; D011441:Propylthiouracil / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013963:Thyroid Hormones / Q000494:pharmacology*; D014284:Triiodothyronine / Q000494:pharmacology
D013956:Antithyroid Agents; D007527:Isoenzymes; D012333:RNA, Messenger; D013963:Thyroid Hormones; D014284:Triiodothyronine; D011441:Propylthiouracil; D010736:Phosphoglycerate Mutase