Alphabeta and gammadelta T cell subsets in chronic renal failure in children on dialysis treatment.
BACKGROUND
Impaired immunity, particularly cell-mediated, is one of the features of chronic renal failure. This also concerns impaired T cell dependent responsiveness.


METHODS
The expression of T cell surface antigens (CD3, CD25, TCRalphabeta, TCRgammadelta) was evaluated on peripheral blood (PB) mononuclear cells using two-color flow cytometry in 10 children on continuous ambulatory peritoneal dialysis (CAPD) and in 13 children on maintenance hemodialysis (HD) with polysulfone and cuprophane dialysers.


RESULTS
In HD children absolute numbers of leukocytes, lymphocytes, CD3+, alphabeta, gammadelta T cells and a percentage of gammadelta T cells were decreased versus healthy children. Also, we observed a relative increase of CD3+, CD3+/CD25+ and alphabeta T cells after sessions with cuprophane membranes, and an increase of CD3+/CD25+, alphabeta T cell percentages after sessions with the polysulfone membranes. Additionally we found a decrease of both relative and absolute numbers of gammadelta T cells after HD with polysulfone. In CAPD children we found declined absolute numbers of total lymphocytes, CD3+ and alphabeta T cells and higher relative values of CD3+ and alphabeta T cells versus controls.


CONCLUSIONS
The T cell depletion in chronic renal failure (CRF) patients primarily results from uremic-related toxicities, rather than from CAPD or HD-related incompatibilities. We showed a significant decrease of gammadelta T cells in CRF patients on HD, that may be partly responsible for impaired T-dependent responsiveness in that group. The intradialytic changes of gammadelta Tcells may result from a different degree of biocompatibility during the application of various dialysis membranes.
Szczepánska
Maria
M
Dialysis Division, Department of Paediatrics, Clinic of Nephrology, Endocrinology and Metabolic Diseases of Childhood, Silesian School of Medicine, ul. 3 Maja 13/15, 41-800 Zabrze, Poland. szczep57@priv5.onet.pl
Szprynger
Krystyna
K
Mazur
Bogdan
B
Szczepánski
Tomasz
T
D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000276:immunology / Q000628:therapy; D018655:Lymphocyte Count; D008297:Male; D010531:Peritoneal Dialysis, Continuous Ambulatory; D006435:Renal Dialysis; D016176:T-Lymphocyte Subsets