Matrix effect on the analysis of oligonucleotides by using a mass spectrometer with a sonic spray ionization source.
Matrix or impurities remaining in a DNA sample solution after various sample treatment procedures may influence a subsequent DNA analysis. In this work, several matrices were investigated concerning their effects on the analysis of oligonucleotide by using an ion-trap mass spectrometer equipped with a sonic spray ionization source. Inorganic salts of sodium chloride and magnesium chloride depressed the signal intensity by about 50% when the content of the salts was about 10 microM. dNTPs and Taq showed more severe depression on the oligonucleotide. However, Tris, or (hydroxymethyl)aminomethane, intensified the signal intensity, if its content was within an appropriate range. When the content of Tris was about 500 microM, the signal intensity was enhanced by factors of 3 and 5 for the 6-mer and the 20-mer oligonucleotides, respectively. With the existence of Tris, matrix effects from the inorganic salts, dNTPs and Taq were reduced.
Huang
M
M
Life Science Group, Hitachi Ltd., Kawagoe, Saitama, Japan. huang@ls.hitachi.co.jp
Hirabayashi
A
A
Okumura
A
A
Hirabayashi
Y
Y
D015224:Dideoxynucleosides / Q000737:chemistry; D015636:Magnesium Chloride / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods*; D019914:Taq Polymerase / Q000737:chemistry; D014325:Tromethamine / Q000737:chemistry; D014867:Water / Q000737:chemistry
D015224:Dideoxynucleosides; D009841:Oligonucleotides; D010710:Phosphates; D014325:Tromethamine; D015636:Magnesium Chloride; D014867:Water; D012965:Sodium Chloride; D019914:Taq Polymerase; D000432:Methanol