Low expression of cyclin D2 in G2/M-arrested and transformed proliferating Balb/3T3 cells.
At present, the effect of cyclin D2 implicated in cell cycle regulation, differentiation, and oncogenic transformation is not fully confirmed. To better elucidate the role of cyclin D2 in controling the cell proliferation, cyclin D2 expression level was determined at the early initiation and promotion stages during the in vitro two-stage transformation process of Balb/3T3 A31 cells. N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced G2/M-arrested cells expressed low level of cyclin D2 mRNA, while the contact-inhibited nonproliferating cells expressed high level of cyclin D2 mRNA. In the transformed proliferating cells at the promotion stage, cyclin D2 mRNA was not expressed. These data suggest that cyclin D2 expression may be associated with the type of growth arrest and nonproliferating state, but not with the cell proliferation and transformation.
Fang
Ming-Zhu
MZ
Laboratory of Toxicology, College of Veterinary Medicine, and School of Agricultural Biotechnology, Seoul National University, Suwon, Korea.
Lee
Moon-Han
MH
Lee
Yong-Soon
YS
Kim
Young-Chul
YC
Lee
Byung-Moo
BM
Cho
Myung-Haing
MH
D016475:3T3 Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D056742:Cyclin D2; D016213:Cyclins / Q000096:biosynthesis* / Q000235:genetics; D016195:G2 Phase / Q000502:physiology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008938:Mitosis / Q000502:physiology; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
C486196:Ccnd2 protein, mouse; D056742:Cyclin D2; D016213:Cyclins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D008769:Methylnitronitrosoguanidine