Expression of insulin-like growth factor (IGF), IGF receptor, and IGF-binding protein messenger ribonucleic acids in luteinized granulosa cells from different size follicles after controlled ovarian hyperstimulation.
OBJECTIVE
This study was conducted to determine the gene expression of insulin-like growth factors (IGFs), their receptors, and binding proteins (IGFBPs) in human luteinized granulosa cells collected during ovum retrieval.


METHODS
cDNA probes were used to analyze the gene expression of IGF-I, IGF-II, IGF type 1 receptor (IGFR-1), IGF type 2 receptor (IGFR-2), and IGFBP-1 to -6 in luteinized granulosa cells.


RESULTS
The levels of IGFBP-5 transcripts of the luteinized granulosa cells increased with increasing follicular fluid (FF) volume when follicles were grouped into 3 volume catagories of < or = 1.5 mL: > 1.5 mL but < or = 4.5 mL; and > 4.5 mL (0.28 +/- 0.64 vs. 0.41 +/- 0.76 vs. 0.46 +/- 0.92, p = 0.037).


CONCLUSIONS
The increased levels of IGFBP-5 transcripts in luteinized granulosa cells found with increased follicular fluid volume after COH may favor a shift toward diminished action of IGFs in larger follicles immediately prior to ovulation.
Chang
Shiuh Y
SY
Medical School, Chang-Gung University, Taoyuan, Taiwan.
Tsai
Meng-Yin
MY
Huang
Fu-Jen
FJ
Kung
Fu-Tsai
FT
D000328:Adult; D001826:Body Fluids / Q000737:chemistry; D005260:Female; D005307:Fertilization in Vitro; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000096:biosynthesis / Q000235:genetics; D018969:Insulin-Like Growth Factor Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D016729:Leuprolide / Q000008:administration & dosage; D034461:Luteinization / Q000235:genetics*; D008596:Menotropins / Q000008:administration & dosage; D006080:Ovarian Follicle / Q000166:cytology*; D010062:Ovulation Induction; D012333:RNA, Messenger / Q000096:biosynthesis; D017451:Receptors, Somatomedin / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013002:Somatomedins / Q000096:biosynthesis* / Q000235:genetics
D018975:Insulin-Like Growth Factor Binding Protein 5; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D017451:Receptors, Somatomedin; D013002:Somatomedins; D008596:Menotropins; D016729:Leuprolide