title: Crystal structure of murine sCEACAM1a[1,4]: a coronavirus receptor in the CEA family.
abstract: CEACAM1 is a member of the carcinoembryonic antigen (CEA) family. Isoforms of murine CEACAM1 serve as receptors for mouse hepatitis virus (MHV), a murine coronavirus. Here we report the crystal structure of soluble murine sCEACAM1a[1,4], which is composed of two Ig-like domains and has MHV neutralizing activity. Its N-terminal domain has a uniquely folded CC' loop that encompasses key virus-binding residues. This is the first atomic structure of any member of the CEA family, and provides a prototypic architecture for functional exploration of CEA family members. We discuss the structural basis of virus receptor activities of murine CEACAM1 proteins, binding of Neisseria to human CEACAM1, and other homophilic and heterophilic interactions of CEA family members.
authors: Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
authors: Kemin
authors: K
authors: Tan
authors: Bruce D
authors: BD
authors: Zelus
authors: Rob
authors: R
authors: Meijers
authors: Jin-huan
authors: JH
authors: Liu
authors: Jeffrey M
authors: JM
authors: Bergelson
authors: Norma
authors: N
authors: Duke
authors: Rongguang
authors: R
authors: Zhang
authors: Andrzej
authors: A
authors: Joachimiak
authors: Kathryn V
authors: KV
authors: Holmes
authors: Jia-huai
authors: JH
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D002272:Carcinoembryonic Antigen / Q000737:chemistry; D015815:Cell Adhesion Molecules; D017124:Conserved Sequence; D017934:Coronavirus / Q000737:chemistry / Q000502:physiology; D018360:Crystallography, X-Ray; D006023:Glycoproteins / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011485:Protein Binding / Q000502:physiology; D020033:Protein Isoforms / Q000737:chemistry; D017434:Protein Structure, Tertiary; D000085064:Receptors, Coronavirus; D011991:Receptors, Virus / Q000737:chemistry*; D016415:Sequence Alignment
chemical_list: D015703:Antigens, CD; C069847:CD66 antigens; D002272:Carcinoembryonic Antigen; C486183:Ceacam1 protein, mouse; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D020033:Protein Isoforms; D000085064:Receptors, Coronavirus; D011991:Receptors, Virus