Interaction of microtubules with active principles of Xanthium strumarium.
Indigenous variety of Xanthium strumarium (X. strumarium) was screened for its antimitotic activity using the microtubule-tubulin system isolated from mammalian tissue. A preliminary phytochemical screening of the whole extracts of the plant was carried out followed by partial purification of the whole extract of X.strumarium. The separated fractions obtained were identified and used for in vitro polymerization studies. The whole as well as partially separated chemical constituents of X. strumarium showed effective inhibition of tubulin polymerization. The results thus suggest that X. strumarium may possess antimitotic components.
Menon
G S
GS
Department of Biochemistry, Institute of Science, Mumbai, India.
Kuchroo
K
K
Dasgupta
D
D
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001921:Brain / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D006160:Guanosine Triphosphate / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D011485:Protein Binding; D013997:Time Factors; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism
D000970:Antineoplastic Agents; D014404:Tubulin; D006160:Guanosine Triphosphate