Detergent extract from chlamydial elementary bodies used as antigen for enzyme-linked immunosorbent assay.
Soluble antigen (SA) from chlamydial elementary bodies (EBs) was extracted with N-lauroylsarcosine. The extracted SA composed of lipopolysaccharide (LPS) and proteins was compared with EBs using an enzyme-linked immunosorbent assay (ELISA). Patient sera from natural chlamydial infections exhibited ELISA mean absorbance (A(492) and A(405/650)) values 2-5 times higher with SA than with EBs, resulting in a better discrimination between positive and negative human sera.
Quevedo Diaz
M A
MA
Institute of Virology, Slovak Academy of Sciences, Dúbravská 9, 84245 Bratislava, Slovak Republic.
Melnicáková
J
J
Kazár
J
J
Hoch
J
J
Senfleben
S
S
Lukácová
M
M
D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000737:chemistry*; D015153:Blotting, Western; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000737:chemistry / Q000276:immunology*; D003902:Detergents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D012521:Sarcosine / Q000031:analogs & derivatives* / Q000737:chemistry
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D003902:Detergents; D008070:Lipopolysaccharides; C025231:sarkosyl; D012521:Sarcosine