Oligomerization process of the hemolytic lectin CEL-III purified from a sea cucumber, Cucumaria echinata.
CEL-III is a Ca(2+)-dependent lectin purified from a sea cucumber, Cucumaria echinata. This protein exhibits strong hemolytic activity as well as cytotoxicity toward some cultured cell lines. Hemolysis is caused by CEL-III oligomers formed in the cell membrane after binding to specific carbohydrate chains on the cell surface. We have found that the oligomerization of CEL-III is also induced by the binding of simple carbohydrates, such as lactose, in aqueous solution under high pH and high ionic strength conditions. From gel filtration analysis of the oligomerization of CEL-III, it was found that the formation of the CEL-III oligomer is effectively induced by the binding of lactose and lactulose, disaccharides containing a beta-galactoside structure. Electron micrographs of the resulting oligomers revealed them to exist as particles with a size of approximately 20-30 nm. The oligomerization process required more than 1 h, which is consistent with the increase in surface hydrophobicity as measured using a fluorescent probe, 8-anilinonaphthalene-1-sulfonate. However, a change in the far-UV CD spectra as well as small-angle X-ray scattering occurred within a few minutes, suggesting that a structural change in the protein takes place rapidly, but the following growth of the oligomer is a much slower process.
Kuwahara
Hiromiki
H
Department of Applied Chemistry, Faculty of Engineering, Nagasaki University Bunkyo-machi Nagasaki 852-8521, Japan.
Yamasaki
Takayuki
T
Hatakeyama
Tomomitsu
T
Aoyagi
Haruhiko
H
Fujisawa
Tetsuro
T
D000817:Anilino Naphthalenesulfonates / Q000737:chemistry; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002850:Chromatography, Gel; D002942:Circular Dichroism; D003603:Cytotoxins / Q000494:pharmacology; D005453:Fluorescence; D006461:Hemolysis; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008854:Microscopy, Electron; D011487:Protein Conformation; D012542:Scattering, Radiation; D012616:Sea Cucumbers / Q000737:chemistry / Q000378:metabolism*; D012997:Solvents; D013997:Time Factors; D014965:X-Rays
D000817:Anilino Naphthalenesulfonates; C402644:CEL-III lectin, Cucumaria echinata; D003603:Cytotoxins; D037102:Lectins; D012997:Solvents; C027132:1-anilino-8-naphthalenesulfonate