title: PCR tools for the differential diagnosis of Taenia saginata and Taenia solium taeniasis/cysticercosis from different geographical locations.
abstract: The potential value of PCRs in the species-specific diagnosis of have been investigated, using samples of T. saginata and T. solium from different geographical areas. The PCRs examining inter-species differences were based on the sequence of the HDP2 DNA fragment, specific for T. saginata/T. solium, and the sequence of the rDNA internal transcribed spacer 1 and spacer 2 (ITS-1 and ITS-2). This PCR analysis of DNA isolates confirmed morphologic diagnosis and allowed the speciation of samples too small or fragmented for morphologic identification, with clear and consistent inter-species differences between T. saginata (twenty-two) and T. solium (three) geographical isolates. Possible intra-species genomic variability, within these species, was similarly studied through analysis of PCR amplification products (PCR-RFLP) and only encountered one exceptional T. saginata isolate from Kenya, which yielded a unique PCR-RFLP pattern, different from T. saginata DNA of Mexican (one sample) and Spanish (seven samples) origin.
authors: Instituto de Salud Carlos III, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain.
authors: Luis Miguel
authors: LM
authors: González
authors: Estrella
authors: E
authors: Montero
authors: Sabino
authors: S
authors: Puente
authors: Rogelio
authors: R
authors: López-Velez
authors: Marisela
authors: M
authors: Hernández
authors: Edda
authors: E
authors: Sciutto
authors: Leslie J S
authors: LJ
authors: Harrison
authors: R Michael E
authors: RM
authors: Parkhouse
authors: Teresa
authors: T
authors: Gárate
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002417:Cattle; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007630:Kenya; D008297:Male; D008800:Mexico; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013030:Spain; D013045:Species Specificity; D013552:Swine; D013621:Taenia / Q000737:chemistry / Q000235:genetics / Q000254:growth & development*; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D000907:Antibodies, Bacterial; D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer