Induced DNA recombination by Cre recombinase protein transduction.
Cre is a DNA recombinase that recognizes 34 base-pair loxP sites of recombination. We have developed a cell-permeable Cre recombinase, TATCre, that is capable of mediating deletion of loxP-flanked targets by simply adding TATCre to cell cultures. Thus, TATCre allows efficient induced DNA recombination without the use of a Cre recombinase transgene or any other genetic material and should prove useful for the genetic manipulation of a wide variety of cell types that have been engineered to possess loxP sites.
Joshi
Sunil K
SK
Institute of Molecular Medicine & Genetics and Department of Medicine, Medical College of Georgia, Augusta 30912, USA.
Hashimoto
Kahoko
K
Koni
Pandelakis A
PA
D000818:Animals; D005347:Fibroblasts / Q000378:metabolism; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases