Adaptive correction of depth-induced aberrations in multiphoton scanning microscopy using a deformable mirror.
We demonstrate adaptive aberration correction for depth-induced spherical aberration in a multiphoton scanning microscope with a micromachined deformable mirror. Correction was made using a genetic learning algorithm with two-photon fluorescence intensity feedback to determine the desired shape for an adaptive mirror. For a 40x/0.6 NA long working distance objective, the axial scanning range was increased from 150 mm to 600 mm.
Sherman
L
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Center for Ultrafast Optical Science, 1006 IST Bldg, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI 48109, USA.
Ye
J Y
JY
Albert
O
O
Norris
T B
TB
D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D055095:Optics and Photonics; D017785:Photons; D011791:Quartz / Q000378:metabolism
D011791:Quartz