Increased expression of intranuclear AChE involved in apoptosis of SK-N-SH cells.
Programmed cell death plays an integral role in neurodegenerative diseases such as Alzheimer's disease (AD). Acetylcholinesterase (AChE) was suggested to be neurotoxic in vivo and in vitro and accelerate assembly of amyloid peptide into Alzheimer's fibrils. In our experiments, we found increased AChE expression in apoptotic neuroblastoma SK-N-SH cells after long-term culture. Our results first showed that in apoptotic SK-N-SH cells, AChE aggregated in the nucleus and suppression of AChE expression with antisense oligonucleotide could save the cells from apoptosis. It was also found that caspase-3 activity was parallel with AChE activation in apoptotic SK-N-SH cells. These results suggest that AChE plays an important role in the procession of neuroblastoma cell apoptosis and favor the association between AChE and neuronal apoptosis in AD.
Yang
Lei
L
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, 200031, Shanghai, PR China.
He
Heng-Yi
HY
Zhang
Xue-Jun
XJ
D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism*; D000544:Alzheimer Disease / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology; D016229:Amyloid beta-Peptides / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics*; D001921:Brain / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D018929:Cell Culture Techniques; D002467:Cell Nucleus / Q000201:enzymology* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D009447:Neuroblastoma; D009474:Neurons / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D015854:Up-Regulation / Q000502:physiology
D016229:Amyloid beta-Peptides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D000110:Acetylcholinesterase; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases