title: Biological significance of proliferation, apoptosis, cytokines, and monocyte/macrophage cells in bone marrow biopsies of 145 patients with myelodysplastic syndrome.
abstract: Labeling index (LI), apoptosis, levels of 2 pro-apoptotic cytokines tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta(TGF-beta), and the number of monocyte/macrophage cells that are the likely source of the cytokines were simultaneously measured in plastic-embedded bone marrow (BM) biopsy sections of 145 patients with myelodysplastic syndromes (MDS). TNF-alpha was correlated with TGF-beta (P = .001) and with monocyte/macrophage cells (P = .003). Patients with excess blasts in their marrows had a higher TGF-beta level (P = .01) and monocyte/macrophage number (P = .05). In a linear regression model,TGF-beta emerged as the most significant biological difference between patients who have excess of blasts and those who do not (P = .01). We conclude that in addition to TNF-alpha, TGF-beta also plays a significant role in the initiation and pathogenesis of MDS, and that a more precise definition of its role will likely identify better preventive and therapeutic strategies.
authors: Rush Cancer Institute, Rush-Presbyterian-St Luke's Medical Center, Chicago, Illinois 60612, USA.
authors: Krishnan
authors: K
authors: Allampallam
authors: Vilasini
authors: V
authors: Shetty
authors: Suneel
authors: S
authors: Mundle
authors: Diya
authors: D
authors: Dutt
authors: Howard
authors: H
authors: Kravitz
authors: Poluru L
authors: PL
authors: Reddy
authors: Sairah
authors: S
authors: Alvi
authors: Naomi
authors: N
authors: Galili
authors: Gurveen S
authors: GS
authors: Saberwal
authors: Shalini
authors: S
authors: Anthwal
authors: Maliha W
authors: MW
authors: Shaikh
authors: Aaron
authors: A
authors: York
authors: Azra
authors: A
authors: Raza
mesh_terms: D000753:Anemia, Refractory / Q000473:pathology; D000754:Anemia, Refractory, with Excess of Blasts / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000473:pathology*; D002455:Cell Division; D016207:Cytokines / Q000032:analysis*; D005260:Female; D006801:Humans; D015477:Leukemia, Myelomonocytic, Chronic / Q000473:pathology; D008264:Macrophages / Q000473:pathology*; D008297:Male; D009000:Monocytes / Q000473:pathology*; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D012044:Regression Analysis; D016196:S Phase; D016212:Transforming Growth Factor beta / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D016207:Cytokines; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha