Postcolumn derivatization method for determination of reducing and phosphorylated sugars in chicken by high performance liquid chromatography.
A postcolumn derivatization method is described for determination of reducing sugars and phosphorylated reducing sugars from chicken meat and other foods using high-performance liquid chromatography (HPLC). Reducing sugars are extracted with ethanol/water, separated on a Kromasil amine-bonded column by isocratic analysis using acetonitrile/water as the mobile phase, and, after postcolumn reaction with tetrazolium blue, are determined by the resulting absorbance at 550 nm. Phosphorylated sugars are first dephosphorylated using alkaline phosphatase and then determined by the same method.
Aliani
Michel
M
Department of Food Science, Queen's University Belfast, and DARD Food Science Division, Newforge Lane, Belfast BT9 5PX, Northern Ireland, UK.
Farmer
Linda J
LJ
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005632:Fructose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D019298:Glucose-6-Phosphate / Q000032:analysis; D018482:Muscle, Skeletal / Q000737:chemistry; D011202:Poultry Products / Q000032:analysis*; D011786:Quality Control; D012266:Ribose / Q000032:analysis; D012267:Ribosemonophosphates / Q000032:analysis; D013403:Sugar Phosphates / Q000032:analysis*
D002241:Carbohydrates; D012267:Ribosemonophosphates; D013403:Sugar Phosphates; D005632:Fructose; C031626:ribose-5-phosphate; D019298:Glucose-6-Phosphate; D012266:Ribose; D000469:Alkaline Phosphatase; D005947:Glucose