Mouse restin inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis.
Restin, a homologous protein of endostatin, was found by Ramchandran et al. It was the C-terminal fragment of type XV collagen. To analysis the inhibition activity of mouse restin on the proliferation of endothelial cells, the cDNA of restin was amplified from the total RNA of the mouse muscle and cloned into the prokaryotic expression plasmid pQE32. The recombinant protein was expressed in inclusion body with a yield about 60%--70% of total protein. After refolding, the purified recombinant protein specifically inhibits bovine aortic endothelial (BAE) cell proliferation stimulated by basic fibroblast growth factor (bFGF) in a dose-dependent manner, but the activity of restin was weaker than that of endostatin. Treatment of BAE cell with recombinant restin caused G(1) arrest and apoptosis in BAE cells.
Xu
Ren
R
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Science, Shanghai 200031, China.
Xin
Li
L
Fan
Yi
Y
Meng
Hai-Rong
HR
Li
Zai-Ping
ZP
Gan
Ren-Bao
RB
D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects; D017209:Apoptosis / Q000187:drug effects*; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D003094:Collagen / Q000235:genetics / Q000494:pharmacology; D043170:Collagen Type XVIII; D004305:Dose-Response Relationship, Drug; D043169:Endostatins; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D010446:Peptide Fragments / Q000235:genetics / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid
D043170:Collagen Type XVIII; D043169:Endostatins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C076744:cytoplasmic linker protein 170; D003094:Collagen