Microbiological characterisation of Burkholderia cepacia isolates from cystic fibrosis patients: investigation of the exopolysaccharides produced.
Eleven strains of Burkholderia cepacia were isolated directly from clinical specimens: 10 from sputum of cystic fibrosis patients, and one from a vaginal swab. They were biochemically identified using API20NE and confirmed by a PCR-based assay. The genomovar characterisation obtained by specific PCR amplification revealed seven strains belonging to genomovar I, three belonging to genomovar IIIA and one belonging to genomovar IV. All isolates were also typed by ribotyping and random amplification of polymorphic DNA analysis. Some of the characterised strains were examined for the ability to produce exopolysaccharides, with the aim of correlating the genomovar with the exopolysaccharide structure. The polysaccharides were analysed by means of methylation analysis and 1H-NMR spectroscopy in order to determine structural similarities. It was shown that different strains are capable of producing chemically different polysaccharides.
Lagatolla
Cristina
C
Dipartimento di Scienze Biomediche, Università di Trieste, via Fleming 22, I-34127, Italy.
Skerlavaj
Silvia
S
Dolzani
Lucilla
L
Tonin
Enrico A
EA
Monti Bragadin
Carlo
C
Bosco
Marco
M
Rizzo
Roberto
R
Giglio
Luisella
L
Cescutti
Paola
P
D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D019121:Burkholderia Infections / Q000150:complications / Q000382:microbiology*; D016956:Burkholderia cepacia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002240:Carbohydrate Sequence; D002648:Child; D003550:Cystic Fibrosis / Q000150:complications*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008745:Methylation; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D018410:Pneumonia, Bacterial / Q000150:complications / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D019105:Random Amplified Polymorphic DNA Technique; D016585:Vaginosis, Bacterial / Q000382:microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011135:Polysaccharides, Bacterial