[Molecular-ecological technology of microorganisms and its application to research on environmental pollution].
Nucleicacid probe detection, PCR technique using primer, DNA sequential analysis, and electrophoresis separation and display were summarized and the application of these techniques to research on environmental pollution and future developing directions were discussed. It was pointed out that molecular-ecological technology of microorganisms are playing an important role in studying on relationships between microorganisms and contaminated environment. There are some important advances such as genetic adaptation and evolutionary mechanisms of microorganisms in contaminated environment, the positioning of pollutant-degrading genes of microorganisms and the construction of microbiological engineering bacteria, thus promoting the development of molecular ecology for bioremediation of contaminated environment.
Zhong
Ming
M
Open Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016. mingzh@mail.sy.ln.cn
Zhou
Qixing
Q
D001419:Bacteria / Q000378:metabolism; D004463:Ecology; D004787:Environmental Pollution; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA