title: Intestinal energy metabolism after ischemia-reperfusion: Effects of moderate hypothermia and perfluorocarbons.
abstract: OBJECTIVE
  This study investigated the roles of moderate hypothermia and extraluminal oxygenated perfluorcarbon (PFC) on intestinal metabolism after ischemia-reperfusion.
  
  
  METHODS
  A model of 30-minute intestinal ischemia followed by 60 minutes of reperfusion was used. The animals were maintained at either normothermia (36.5 to 37.5 degrees C) or moderate hypothermia (31 to 32 degrees C). Four groups of adult rats were studied (n = 8 per group): (A) sham at normothermia, (B) ischemia-reperfusion at normothermia, (C) ischemia-reperfusion at hypothermia and, (D) ischemia-reperfusion with extraluminal oxygenated PFC perfusion during ischemia at normothermia. Intestinal phosphocreatine, ATP and lactate levels were measured. Histologic changes in the intestine were evaluated.
  
  
  RESULTS
  Intestinal ischemia-reperfusion at normothermia caused a marked reduction in phosphocreatine and ATP with an increase in lactate. Moderate hypothermia exerted beneficial effects by attenuating the depletion of high-energy phosphates and the elevation of lactate. Extraluminal PFC perfusion during ischemia failed to produce a protective effect on high-energy phosphates, although it reduced lactate accumulation. Moderate hypothermia significantly decreased the degree of mucosal damage.
  
  
  CONCLUSIONS
  Whole-body moderate hypothermia protects the small intestine from reperfusion injury as measured both biochemically and histologically. Extraluminal oxygenated PFC administration during ischemia did not protect the intestine from reperfusion injury in this model.
authors: London, England.
authors: Paisarn
authors: P
authors: Vejchapipat
authors: Edward
authors: E
authors: Proctor
authors: Alan
authors: A
authors: Ramsay
authors: Andy
authors: A
authors: Petros
authors: David G
authors: DG
authors: Gadian
authors: Lewis
authors: L
authors: Spitz
authors: Agostino
authors: A
authors: Pierro
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004734:Energy Metabolism; D005466:Fluorocarbons / Q000008:administration & dosage; D007036:Hypothermia, Induced; D007082:Ileum / Q000473:pathology; D007413:Intestinal Mucosa / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000628:therapy*
chemical_list: D005466:Fluorocarbons; D010725:Phosphocreatine; D019344:Lactic Acid; D000255:Adenosine Triphosphate