Gene for integrin-associated protein (IAP, CD47): physical mapping, genomic structure, and expression studies in skeletal muscle.
Integrin-associated protein (IAP) is a widely expressed membrane protein with multiple functions in immunological and neuronal processes. Having physically mapped the IAP gene into a BAC/PAC contig covering approximately 1 Mb on human chromosome 3ql3.1-q13.2, we determined the genomic organization of the gene, established its expression in skeletal muscle, and identified a novel splice variant. Our expression studies demonstrate expression of integrin-associated protein in the t-tubular system and the euchromatin of skeletal muscle cells where its function thus far is not known.
Schickel
Jörg
J
Institut für Klinische Chemie und Laboratoriumsdiagnostik, Klinikum der Friedrich-Schiller Universität Jena, Germany. joerg.schickel@med.uni-jena.de
Stahn
Katharina
K
Zimmer
Klaus-Peter
KP
Sudbrak
Ralf
R
Størm
Tim M
TM
Dürst
Matthias
M
Kiehntopf
Michael
M
Deufel
Thomas
T
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000378:metabolism; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics*; D001665:Binding Sites; D051928:CD47 Antigen; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3; D005091:Exons; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D020161:Physical Chromosome Mapping; D013347:Subcellular Fractions / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D051928:CD47 Antigen; C497894:CD47 protein, human; D002352:Carrier Proteins