Isolation of oligosaccharides from a partial-acid hydrolysate of pneumococcal type 3 polysaccharide for use in conjugate vaccines.
A series of well-defined oligosaccharide fragments of the capsular polysaccharide of Streptococcus pneumoniae type 3 has been generated. Partial-acid hydrolysis of the capsular polysaccharide, followed by fractionation of the oligosaccharide mixture by Sepharose Q ion-exchange chromatography yielded fragments containing one to seven [-->3)-beta-D-GlcpA-(1-->4)-beta-D-Glcp-(1-->] repeating units. The isolated fragments were analysed for purity by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using an IonPac AS11 column, and their structures were verified by 1H NMR spectroscopy and nano-electrospray mass spectrometry. The oligosaccharides can be used to produce neoglycoprotein vaccines with a defined carbohydrate part.
Lefeber
Dirk J
DJ
Department of Bio-Organic Chemistry, Bijvoet Center, Utrecht University, PO Box 80.075, NL-3508 TB, The Netherlands.
Gutiérrez Gallego
Ricardo
R
Grün
Christian H
CH
Proietti
Daniela
D
D'Ascenzi
Sandro
S
Costantino
Paolo
P
Kamerling
Johannis P
JP
Vliegenthart
Johannes F G
JF
D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000737:chemistry*; D018074:Vaccines, Conjugate / Q000737:chemistry*
D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D018074:Vaccines, Conjugate