PCR-based detection of resistance to acetyl-CoA carboxylase-inhibiting herbicides in black-grass (Alopecurus myosuroides Huds) and ryegrass (Lolium rigidum gaud).
A simple method based upon allele-specific PCR was developed to detect an isoleucine-leucine substitution in the gene encoding chloroplastic acetyl-coenzyme A carboxylase (ACCase) in two gramineous weeds: Lolium rigidum Gaud and Alopecurus myosuroides Huds. Analysis of 1800 A myosuroides and 750 L rigidum seedlings showed that the presence of ACCase leucine allele(s) conferred cross-resistance to the cyclohexanedione herbicide cycloxydim and to the aryloxyphenoxypropionate herbicides fenoxaprop-P-ethyl and diclofop-methyl. Seedlings containing ACCase leucine allele(s) could be either sensitive or resistant to the aryloxyphenoxypropionate herbicides haloxyfop-P-methyl and clodinafop-propargyl. Successful detection of resistant plants in a field population of A myosuroides was achieved using this PCR assay. Using it with basic molecular biology laboratory equipment, the presence of resistant leucine ACCase allele(s) can be detected within one working day.
Délye
Christophe
C
Institut National de la Recherche Agronomique, Laboratoire de Malherbologie et Agronomie, BP 86510, 21065 Dijon, France. delye@dijon.inra.fr
Matéjicek
Annick
A
Gasquez
Jacques
J
D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors*; D000483:Alleles; D019943:Amino Acid Substitution; D001681:Biological Assay; D004351:Drug Resistance / Q000235:genetics; D006540:Herbicides / Q000378:metabolism* / Q000633:toxicity; D008129:Lolium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020125:Mutation, Missense; D006109:Poaceae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011422:Propionates / Q000378:metabolism / Q000633:toxicity; D011725:Pyridines / Q000378:metabolism / Q000633:toxicity; D012680:Sensitivity and Specificity
D006540:Herbicides; D011422:Propionates; D011725:Pyridines; C054176:haloxyfop; D000103:Acetyl-CoA Carboxylase; C400760:clodinafop