The recent history of somatic cloning in mammals.
The history of somatic cell nuclear transfer (NT) in mammals is full of exciting experiments and findings regarding the technique and outcome of NT, despite only covering a period of 6 years. The production of Dolly, for the first time demonstrating cloning from an adult somatic cell, had a great impact on subsequent studies. However, the more progress we make, the more obvious it becomes how little we know about the processes during NT, specifically how reprogramming events occur. Therefore, it is certainly challenging to continue investigating every step of somatic cell NT more intensively, starting from the donor cell, (type, cell cycle, synchronization, population doublings) and continuing until the cloned offspring are born and even further, to see how and if NT has an influence on health, viability, quantitative traits, and reproduction of cloned individuals.
Brem
Gottfried
G
Ludwig Bolzmann Institute of Immuno-, Cyto- and Moleculargenetic Research, Vienna, Austria. gbremabg@netscape.net
Kühholzer
Birgit
B
D000818:Animals; D019976:Cloning, Organism / Q000639:trends; D004624:Embryo Transfer / Q000639:trends; D015870:Gene Expression; D053652:Nuclear Transfer Techniques; D012756:Sheep; D013997:Time Factors