title: Chemically modified tetracycline (CMT-8) and estrogen promote wound healing in ovariectomized rats: effects on matrix metalloproteinase-2, membrane type 1 matrix metalloproteinase, and laminin-5 gamma2-chain.
abstract: Estrogen deficiency is associated with impaired cutaneous wound healing. Remodeling of the extracellular matrix in wound healing involves the action of matrix metalloproteinases on basement membrane zone components, especially laminin-5. We studied the effects of estrogen and a potent matrix metalloproteinase inhibitor, chemically modified non-antimicrobial tetracycline, CMT-8, on wound healing in ovariectomized rats. At the tissue level, laminin-5 gamma2-chain expression was decreased and the migration-inductive 80 kDa form of laminin-5 gamma2-chain was absent in ovariectomized rats when compared with sham and CMT-8- or estrogen-treated ovariectomized animals as detected by Western blotting. The highest levels of gelatinolytic activity (matrix metalloproteinase-2 and -9) were found in sham animals. Levels were reduced in ovariectomized rats and were lowest after treating ovariectomized rats with CMT-8 or estrogen as analyzed by functional activity assay and zymography. The total amount of membrane type 1-matrix metalloproteinase was unchanged in all groups. We conclude that CMT-8 and estrogen can promote wound healing in ovariectomized rats, not only by normalizing wound bed total collagen content and structure, but also by recovering the expression and processing of key molecules in wound healing, i.e., laminin-5 gamma2-chain. This study shows, for the first time, the role of estrogen and CMT-8 in laminin-5 gamma2-chain modulation in vivo.
authors: Department of Clinical Veterinary Sciences, University of Helsinki, Finland. pirila@hammas.helsinki.fi
authors: Emma
authors: E
authors: Pirilä
authors: Mataleens
authors: M
authors: Parikka
authors: Nungavarm S
authors: NS
authors: Ramamurthy
authors: Päivi
authors: P
authors: Maisi
authors: Steve
authors: S
authors: McClain
authors: Allan
authors: A
authors: Kucine
authors: Taina
authors: T
authors: Tervahartiala
authors: Kaiu
authors: K
authors: Prikk
authors: Lorne M
authors: LM
authors: Golub
authors: Tuula
authors: T
authors: Salo
authors: Timo
authors: T
authors: Sorsa
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004817:Epidermis / Q000378:metabolism; D004967:Estrogens / Q000502:physiology*; D005260:Female; D018093:Gelatinases / Q000378:metabolism; D022002:Hemidesmosomes / Q000502:physiology; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D023421:Models, Animal; D010052:Ovariectomy; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013754:Tetracyclines / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology*; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D004967:Estrogens; D018093:Gelatinases; D020781:Matrix Metalloproteinase 1; D020778:Matrix Metalloproteinase 2; D011480:Protease Inhibitors; D013754:Tetracyclines; D000097794:Kalinin; C117153:tetracycline CMT-8