Mixed urease/amphiphile LB films and their application for biosensor development.
The optimal conditions for the formation of a stable mixed urease/octadecylamine (ODA) film at the air-water interface are determined. This film is efficiently transferred onto the hydrophobized surface of a pH-ISFET, and the features of the urea enzymatic field-effect transistor (ENFET) are determined: detection limit 0.2 mM, response time 15 s, and dynamic range 0-20 mM.
Zhang
Aidong
A
IFOS-LPC1, UMR CNRS 5621, Ecole Centrale de Lyon, 36 Avenue Guy de Collongue, BP 163, F69131 Ecully Cedex, France.
Hou
Yanxia
Y
Jaffrezic-Renault
Nicole
N
Wan
Jialiang
J
Soldatkin
Alexey
A
Chovelon
Jean-Marc
JM
D000588:Amines / Q000737:chemistry*; D015374:Biosensing Techniques; D017550:Spectroscopy, Fourier Transform Infrared; D014510:Urease / Q000737:chemistry*
D000588:Amines; D014510:Urease; C009317:stearylamine