Cyclic voltammetry study of glucose and insulin interactions with supported lipid membrane.
The effect of D-glucose and insulin on conducting properties of supported bilayer lipid membranes (s-BLM) modified by anthraquinone-2-sulphonic acid (AQS) at the presence of potassium ferricyanide was studied by means of cyclic voltammetry (CV). Both the oxidation and the reduction current peaks were found to decrease at the presence of glucose in concentration range varying from 10 to 320 mM. The influence of insulin on membrane properties is ambiguous. While the pretreatment of membrane with 20 mU l(-1) of insulin evoked slight increase of the current with unchanged course of the dependence of peak current on glucose, the decrease of conductance was observed above 10(5) mU l(-1) of insulin.
Laputková
G
G
Department of Medical Biophysics, Medical Faculty, University of P.J. Safárik, Trieda SNP 1, 040 66, Kosice, Slovak Republic.
Sabo
J
J
D000880:Anthraquinones / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D005947:Glucose / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*
D000880:Anthraquinones; D007328:Insulin; D008051:Lipid Bilayers; C023554:anthraquinone sulfonate; D005947:Glucose