The complete genome sequence of Perina nuda picorna-like virus, an insect-infecting RNA virus with a genome organization similar to that of the mammalian picornaviruses.
Perina nuda picorna-like virus (PnPV) is an insect-infecting RNA virus with morphological and physicochemical characters similar to the Picornaviridae. In this article, we determine the complete genome sequence and analyze the gene organization of PnPV. The genome of PnPV consists of 9476 nucleotides (nts) excluding the poly(A) tail and contains a single large open reading frame (ORF) of 8958 nts (2986 codons) flanked by 473 and 45 nt noncoding regions on the 5' and 3' ends, respectively. Northern blotting did not detect the presence of any subgenomic RNA. The PnPV genome codes for four structural proteins (CP1-4), and determination of their N-terminal sequences by Edman degradation, showed that all four are located in the 5' region of the genome. The 3' part of the PnPV genome contains the consensus sequence motifs for picornavirus RNA helicase, cysteine protease, and RNA-dependent RNA polymerase (RdRp) in that order from the 5' to the 3' end. In all of these characters, the genome organization of PnPV resembles the mammalian picornaviruses and two other insect picorna-like viruses, infectious flacherie virus (IFV) of the silkworm and Sacbrood virus (SBV) of the honeybee. In a phylogenetic tree based on the eight conserved domains in the RdRp sequence, PnPV formed a separate cluster with IFV and SBV, which suggests that these three insect picorna-like viruses might constitute a novel group of insect-infecting RNA viruses.
Wu
Chih-Yu
CY
Department of Entomology, National Taiwan University, Taipei, Taiwan, Republic of China.
Lo
Chu-Fang
CF
Huang
Chang-Jen
CJ
Yu
Hon-Tsen
HT
Wang
Chung-Hsiung
CH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002213:Capsid / Q000235:genetics; D002460:Cell Line; D002874:Chromosome Mapping; D003546:Cysteine Endopeptidases / Q000235:genetics; D004279:DNA, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny; D010849:Picornaviridae / Q000145:classification / Q000235:genetics*; D021382:Protein Sorting Signals; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014764:Viral Proteins
D004279:DNA, Viral; D021382:Protein Sorting Signals; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; C057400:picornain 2A, Picornavirus