Isolation of a biodegradable sterol-rich fraction from industrial wastes.
Several industrial waste materials were screened for their sterol content. The possibility of using these industrial by-products as sterol sources for the microbiological production of 4-androsten-3,17-dione (AD) and 1,4-androsta-diene-3,17-dione (ADD) was investigated. Two methods of obtaining the sterol fraction from wastes were developed. Sterol-rich (96-98%) fractions were isolated in a yield above 70%, from a tall-oil effluent of a paper pulp industry and from edible-oil deodorizates. These fractions were subsequently used as a substrate for microbial degradation by a Mycobacterium sp. strain and proved to be easily converted to AD and ADD.
Dias
A C P
AC
Departamento de Biologia, Universidade do Minho, Braga, Portugal.
Fernandes
P
P
Cabral
J M S
JM
Pinheiro
H M
HM
D000735:Androstenedione / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D007220:Industrial Waste; D009161:Mycobacterium / Q000378:metabolism; D010938:Plant Oils; D013261:Sterols / Q000302:isolation & purification*; D013997:Time Factors; D014867:Water / Q000737:chemistry
D007220:Industrial Waste; D010938:Plant Oils; D013261:Sterols; D014867:Water; C482568:tall oil; D000735:Androstenedione