Age-related differences in pulmonary inflammatory responses to JP-8 jet fuel aerosol inhalation.
Our previous studies have demonstrated that JP-8 jet fuel aerosol inhalation induced lung injury and dysfunction. To further examine JP-8 jet fuel-induced inflammatory mechanisms, a total of 40 male C57BL/6 mice (young, 3.5 months; adult, 12 months; half in each age group) were randomly assigned to the exposure or control groups. Mice were nose-only exposed to room air or atmospheres of 1000 mg/m3 JP-8 jet fuel for 1 h/day for 7 days. Lung injury was assessed by pulmonary mechanics, respiratory permeability, lavaged cell profile, and chemical mediators in bronchoalveolar lavage fluid (BALF). The young and adult mice exposed to JP-8 jet fuel had similar values with regards to increased lung dynamic compliance, lung permeability, BALF cell count, and decreased PGE2. However, there were several different responses between the young-versus-adult mice with respect to BALF cell differential, TNF-alpha, and 8-iso-PGF2,, levels after exposure to JP-8 jet fuel. These data suggest that JP-8 jet fuel may have different inflammatory mechanisms leading to lung injury and dysfunction in the younger-versus-adult mice.
Wang
S
S
The Joan B. and Donald R. Diamond Lung Injury Laboratory, Department of Pediatrics, The University of Arizona College of Medicine, Tucson, USA.
Young
R S
RS
Witten
M L
ML
D000280:Administration, Inhalation; D000336:Aerosols / Q000008:administration & dosage; D000367:Age Factors; D000403:Airway Resistance; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D006838:Hydrocarbons / Q000008:administration & dosage / Q000633:toxicity*; D018836:Inflammation Mediators / Q000032:analysis; D016676:Macrophages, Alveolar / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010539:Permeability; D011014:Pneumonia / Q000139:chemically induced* / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology
D000336:Aerosols; D006838:Hydrocarbons; D018836:Inflammation Mediators; C098607:JP8 aviation fuel