title: Effect of mild acid pH on the functioning of bacterial membranes in Vibrio cholerae.
abstract: In this paper, we initiated the first two-dimensional electrophoresis map of Vibrio cholerae, the aetiological agent of cholera disease. In this pathogen the efficient adaptation to detrimental conditions plays an important role in its survival in both the aquatic reservoir and human intestine. By proteome analysis we investigated the effect of mild acid treatment on the physiology of V. cholerae. More than 50 proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and database searching. Amongst them, pH regulated proteins belong to various functional classes such as intermediary metabolism and bacterial envelope. Several proteins whose accumulation level was decreased in response to acidic pH are known to be involved in the organization and the functioning of membranes, including lipopolysaccharide. Consistent with this, we observed an increased susceptibility to hydrophobic drugs, a loss of motility and a reduction in the ability to form a biofilm in cells grown at pH 6. Our results suggest that V. cholerae is able to sense a moderate decrease in pH and to modify accordingly its structure and physiology.
authors: Unité de Génétique des Génomes Bactériens, Institut Pasteur, Paris, France.
authors: Florence
authors: F
authors: Hommais
authors: Christine
authors: C
authors: Laurent-Winter
authors: Valérie
authors: V
authors: Labas
authors: Evelyne
authors: E
authors: Krin
authors: Christian
authors: C
authors: Tendeng
authors: Olga
authors: O
authors: Soutourina
authors: Antoine
authors: A
authors: Danchin
authors: Philippe
authors: P
authors: Bertin
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D018441:Biofilms; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D048429:Cell Size; D030541:Databases, Genetic; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010641:Phenotype; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014734:Vibrio cholerae / Q000737:chemistry / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome