title: Cryopreserved chronic lymphocytic leukemia cells analyzed by multicolor fluorescence in situ hybridization after optimized mitogen stimulation.
abstract: We investigated the utility of multicolor in situ fluorescence hybridization (mFISH) on cryopreserved blood cells from 11 chronic lymphocytic leukemia (CLL) patients. The results demonstrate that an individually chosen optimized mitogen combination induces proliferation of neoplastic B-cells after cryopreservation. Abnormal cells were detected in eight samples by mFISH, and, in six samples, the abnormality could be verified by comparative genomic hybridization or interphase FISH. In addition to typical CLL abnormalities, such as del(11q) or +12, several balanced translocations and single-cell abnormalities were found. Thus, mFISH can reveal new prognostically relevant chromosome aberrations in CLL.
authors: Laboratory of Cancer Genetics, University of Tampere and Tampere University Hospital, Tampere, Finland.
authors: Ritva
authors: R
authors: Karhu
authors: Leena
authors: L
authors: Vilpo
authors: Jorma
authors: J
authors: Isola
authors: Sakari
authors: S
authors: Knuutila
authors: Juhani
authors: J
authors: Vilpo
mesh_terms: D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002869:Chromosome Aberrations; D020223:Chromosome Painting; D015925:Cryopreservation; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008934:Mitogens / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D008934:Mitogens