title: Reductive deamination as a new step in the anaerobic microbial degradation of halogenated anilines.
abstract: In this paper we report the isolation and characterization of an anaerobic enrichment culture as well as of a Rhodococcus sp. strain 2 capable of degrading 3,4-dihaloanilines under nitrate reducing conditions. Using mass spectrometry several of the intermediates formed in the process of 3,4-dichloroaniline conversion were identified. Most interesting is the observation of reductive deamination and the formation of 1,2-dichlorobenzene as one of the intermediates. Using 19F NMR and fluorinated 3,4-dihaloaniline model substrates it was corroborated that reductive deamination of the anilines to give dihalobenzene intermediates represents a new initial step in the anaerobic microbial degradation of these halogenated anilines.
authors: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russian Federation.
authors: Vasili
authors: V
authors: Travkin
authors: Boris P
authors: BP
authors: Baskunov
authors: Eugene L
authors: EL
authors: Golovlev
authors: Marelle G
authors: MG
authors: Boersma
authors: Sjef
authors: S
authors: Boeren
authors: Jacques
authors: J
authors: Vervoort
authors: Willem J H
authors: WJ
authors: van Berkel
authors: Ivonne M C M
authors: IM
authors: Rietjens
authors: Ludmila A
authors: LA
authors: Golovleva
mesh_terms: D000693:Anaerobiosis; D000814:Aniline Compounds / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004785:Environmental Pollutants / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012240:Rhodococcus / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000814:Aniline Compounds; D004785:Environmental Pollutants; C014464:3,4-dichloroaniline; C025654:3-chloro-4-fluoroaniline