Characterisation of the diol dehydratase pdu operon of Lactobacillus collinoides.
The three genes (pduCDE) encoding the diol dehydratase of Lactobacillus collinoides were sequenced. They exhibited strong identities with the ddrABC and pduCDE genes of Klebsiella oxytoca and Salmonella enterica, respectively. These genes are part of a putative operon with at least four other genes. An eighth open reading frame was identified as homologous to the pocR gene (encoding the operon regulatory protein). Although the enzyme was detected in exponential growth phase, PduCDE activity was increased at the end of exponential phase in presence of 1,2-propanediol.
Sauvageot
Nicolas
N
USC INRA de Microbiologie de l'Environnement, Université de Caen, 14032 Caen Cedex, France. phdlme@ibba.unicaen.fr
Muller
Cécile
C
Hartke
Axel
A
Auffray
Yanick
Y
Laplace
Jean-Marie
JM
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics; D004790:Enzyme Induction / Q000187:drug effects; D005798:Genes, Bacterial; D007709:Klebsiella / Q000201:enzymology / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics; D007778:Lactobacillus / Q000201:enzymology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D011408:Propanediol Dehydratase / Q000235:genetics / Q000378:metabolism*; D019946:Propylene Glycol / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019779:Salmonella enterica / Q000201:enzymology / Q000235:genetics; D013045:Species Specificity; D015534:Trans-Activators
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C084166:PocR protein, Salmonella; D015534:Trans-Activators; D019946:Propylene Glycol; D011408:Propanediol Dehydratase