Comparison of different methods for diagnosis of porcine proliferative enteropathy.
The objectives of this study were: (1) to compare 2 methods of serology; (2) to compare 3 histologic techniques; and (3) to compare 2 methods of detecting shedding in pigs experimentally challenged with Lawsonia intracellularis. The sensitivities of these tests were determined by the detection of infection. Forty 5-week-old pigs were inoculated on day 0 with intestinal homogenate from pigs with proliferative enteropathy (PE). Clinical evaluation was done on day 7 and daily from day 14 to 28 postinoculation. Fecal shedding of L. intracellularis was monitored by use of polymerase chain reaction (PCR) analysis and immunoperoxidase staining at 7-day intervals. Serum was obtained on days 0 and 28 for serologic testing by glass slide and tissue culture indirect fluorescent antibody tests. At euthanasia on day 28, gross intestinal lesions were evaluated and ileum samples collected for histologic analyses. Ileal histologic sections from each animal were stained by hematoxylin and eosin, Warthin-Starry silver stain, and immunohistochemistry (IHC). Of the 40 pigs, 36 had gross lesions typical of PE at necropsy. The percentage of agreement between the 2 serologic methods was 94.4%. Immunoperoxidase stain of fecal smears was more sensitive than PCR for detecting fecal shedding, especially on day 21 (89.5% and 60.5%, respectively) and day 28 (59.4% and 37.5%, respectively) post-inoculation. The IHC stain was much more sensitive for detecting infection than the routinely used hematoxylin and eosin and Warthin-Starry silver stains. In conclusion, in experimentally infected pigs, both serologic methods were appropriate techniques for detecting infection. For fecal samples, PCR has low sensitivity. Immunohistochemistry is the best diagnostic tool for formalin-fixed samples.
Guedes
Roberto M C
RM
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, Saint Paul 55108, USA. gued0001@tc.umn.edu
Gebhart
Connie J
CJ
Winkelman
Nathan L
NL
Mackie-Nuss
Rebecca A C
RA
Marsteller
Thomas A
TA
Deen
John
J
D000818:Animals; D005243:Feces / Q000382:microbiology; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000662:veterinary*; D007082:Ileum / Q000382:microbiology / Q000473:pathology; D007124:Immunoenzyme Techniques / Q000379:methods / Q000662:veterinary; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary; D007410:Intestinal Diseases / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D020639:Lawsonia Bacteria; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology