title: Effects of cigarette smoking with different tar content on erythrocyte oxidant/antioxidant status.
abstract: In this study, the effects of cigarettes with differing tar content on erythrocyte oxidant/antioxidant status was investigated. Malondialdehyde (MDA) as an indicator of oxidant status and superoxide radical scavenger activity (SSA) as an indicator of antioxidant status were measured in erythrocytes from 20 smokers and 10 non-smoker controls. Ten of the 20 smoking subjects smoked five cigarettes with full flavour low tar (FFLT with 12 mg tar) and the others smoked five cigarettes with full flavour high tar (FF with 23 mg tar) over 1 hour. Initial blood samples from both groups at fasting, followed by further samples from smokers at 1.5 hours and 3 hours after smoking. Initial erythrocyte MDA level and SSA activity were found to be higher in the smoking groups compared to non-smokers. Furthermore, both parameters were significantly higher at the 1.5-hour and 3-hour erythrocyte samples when compared to initial values in the FFLT group. However, there were no statistically significant differences between SSA values established at different times in FF group. Results suggest that smoking causes oxidant load in the erythrocytes. Although a compensatory mechanism (i.e. increased SSA activities) develops in the FFLT group after smoking, this cannot prevent peroxidation reactions (i.e. increased MDA levels) in the erythrocytes. As to the types of cigarettes, both seem to have oxidant potential, but oxidation degree in the FFLT group is higher than that of FF group. These results suggest that antioxidant supplementation to smokers might be beneficial to decrease cellular oxidation damages.
authors: Ankara University Medical Faculty, Department of Biochemistry, Sihhiye, Ankara, Turkey. durak@medicine.ankara.edu.tr
authors: Ilker
authors: I
authors: Durak
authors: Serenay
authors: S
authors: Elgün
authors: N
authors: N
authors: Kemal Bingöl
authors: M Y
authors: MY
authors: Burak Cimen
authors: Murat
authors: M
authors: Kaçmaz
authors: Serap
authors: S
authors: Büyükkoçak
authors: H
authors: H
authors: Serdar Oztürk
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008889:Military Personnel; D012016:Reference Values; D012907:Smoking / Q000009:adverse effects* / Q000097:blood; D013638:Tars / Q000009:adverse effects* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D013638:Tars; C024746:tobacco tar; D008315:Malondialdehyde