Expression of D7 and D7-related proteins in the salivary glands of the human malaria mosquito Anopheles stephensi.
Full-length cDNA clones encoding D7 (AnsD7) and D7-related (AnsD7r1) secreted salivary gland proteins were isolated from Anopheles stephensi. Corresponding proteins were separated by SDS-PAGE and analysed by N-terminal sequencing, which also identified a second D7-related protein (AnsD7r2). AnsD7 encodes a protein of 37 kDa, AnsD7r1 of 18 kDa, and AnsD7r2 of 16 kDa. Polyclonal antibodies against recombinant AnsD7 showed immunological cross-reactivity with the D7-related proteins, and alignment demonstrated sequence similarity between the C-terminal region of AnsD7 and the D7-related proteins. AnsD7, AnsD7r1 and AnsD7r2 were major female-specific salivary gland proteins, and Western blotting, immunohistochemistry and immunogold labelling demonstrated expression was predominantly in the secretory cavities of the distal-lateral and median lobes. Expression and localization of D7 and D7-related proteins was similar in Plasmodium berghei-infected and uninfected mosquitoes.
Suwan
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Division of Molecular Biology and Immunology, Liverpool School of Tropical Medicine, School of Biological Sciences, University of Liverpool, Liverpool, UK.
Wilkinson
M C
MC
Crampton
J M
JM
Bates
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology; D001483:Base Sequence; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015870:Gene Expression; D006801:Humans; D007120:Immunochemistry; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007303:Insect Vectors / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology; D008288:Malaria; D008969:Molecular Sequence Data; D010962:Plasmodium berghei; D012333:RNA, Messenger; D012469:Salivary Glands / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012967:Sodium Dodecyl Sulfate
D018076:DNA, Complementary; D019476:Insect Proteins; D012333:RNA, Messenger; D012967:Sodium Dodecyl Sulfate