Comparison of micellar and microemulsion electrokinetic chromatography for the analysis of water- and fat-soluble vitamins.
Separation and determination of water- and fat-soluble vitamins by micellar (MEKC) and microemulsion electrokinetic chromatography (MEEKC) are compared. MEKC is only useful in the quantitative analysis of water-soluble vitamins when sodium dodecylsulfate (SDS) is used as the surfactant. However, the separation of mixtures containing water- and fat-soluble vitamins is only achieved by MEEKC using a microemulsion prepared by mixing SDS as the surfactant, butanol as the co-surfactant, octane as the non-polar modifier and propanol as the second co-surfactant. The injection time and the solvent used for the dilution of samples have a significant effect on the analysis of lypophilic compounds. The most reproducible results in the analysis of fat-soluble vitamins are obtained by using the same microemulsion electrolyte as the solvent for samples and an injection time of 10 s.
Sánchez
Juan M
JM
Department of Chemistry, University of Girona, Spain.
Salvadó
Victoria
V
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004655:Emulsions; D005223:Fats; D008823:Micelles; D012995:Solubility; D014815:Vitamins / Q000032:analysis*; D014867:Water
D004655:Emulsions; D005223:Fats; D008823:Micelles; D014815:Vitamins; D014867:Water