CTX-m-3 beta-lactamase-producing Escherichia coli from Greece.
An Escherichia coli clinical strain resistant to all beta-lactams except carbapenems was isolated in a Greek hospital. Analysis of beta-lactamase content by isoelectric focusing, PCR assays specific for various bla genes, and DNA sequencing showed that the strain produced TEM-1, a Citrobacter freundii AmpC-related cephalosporinase, and CTX-M-3. The blaCTX.M-3 gene was carried by a 120-kb plasmid that was readily transferable to a susceptible E. coli host.
Mavroidi
A
A
Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.
Tzelepi
E
E
Miriagou
V
V
Gianneli
D
D
Legakis
N J
NJ
Tzouvelekis
L S
LS
D016971:Citrobacter freundii / Q000235:genetics; D004351:Drug Resistance; D004926:Escherichia coli / Q000201:enzymology*; D004927:Escherichia coli Infections / Q000382:microbiology; D006115:Greece; D006801:Humans; D007525:Isoelectric Focusing; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000737:chemistry / Q000378:metabolism*
C417271:beta-lactamase CTX-M-3; D001618:beta-Lactamases