title: The comparison of rapid bioassays for the assessment of urban groundwater quality.
abstract: Groundwater is a complex mixture of chemicals that is naturally variable. Current legislation in the UK requires that groundwater quality and the degree of contamination are assessed using chemical methods. Such methods do not consider the synergistic or antagonistic interactions that may affect the bioavailability and toxicity of pollutants in the environment. Bioassays are a method for assessing the toxic impact of whole groundwater samples on the environment. Three rapid bioassays, Eclox, Microtox and ToxAlert, and a Daphnia magna 48-h immobilisation test were used to assess groundwater quality from sites with a wide range of historical uses. Eclox responses indicated that the test was very sensitive to changes in groundwater chemistry; 77% of the results had a percentage inhibition greater than 90%. ToxAlert, although suitable for monitoring changes in water quality under laboratory conditions, produced highly variable results due to fluctuations in temperature and the chemical composition of the samples. Microtox produced replicable results that correlated with those from D. magna tests.
authors: Department of Geology, Royal Holloway, University of London, Egham, Surrey, UK. r.dewhurst@rhul.ac.uk
authors: R E
authors: RE
authors: Dewhurst
authors: J R
authors: JR
authors: Wheeler
authors: K S
authors: KS
authors: Chummun
authors: J D
authors: JD
authors: Mather
authors: A
authors: A
authors: Callaghan
authors: M
authors: M
authors: Crane
mesh_terms: D000641:Ammonia / Q000633:toxicity; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002712:Chlorides / Q000633:toxicity; D003621:Daphnia / Q000187:drug effects*; D005618:Fresh Water / Q000737:chemistry*; D014507:Urbanization; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D014881:Water Supply / Q000032:analysis*
chemical_list: D002712:Chlorides; D014874:Water Pollutants, Chemical; D000641:Ammonia