title: Cultivation of hematopoietic stem and progenitor cells: biochemical engineering aspects.
abstract: The ex vivo expansion of hematopoietic cells is one of the most challenging fields in cell culture. This is a rapidly growing area of tissue engineering with many potential applications in bone marrow transplantation, transfusion medicine or gene therapy. Over the last few years much progress has been made in understanding hematopoietic differentiation, discovery of cytokines, isolation and identification of cellular subtypes and in the development of a variety of bioreactor concepts. All this has led to a number of (preliminary) clinical trials that gave a hint of the benefits that can be obtained from the use of expanded hematopoietic cells in therapy. Moreover, as we understand the complexity and the regulation of hematopoiesis, it becomes obvious that highly sophisticated cultivation techniques and bioreactor concepts are needed: a new challenge for bioprocess engineering in cell culture.
authors: Institut für Biotechnologie 2, Forschungszentrum Jülich GmbH, Germany. th.noll@fz-juelich.de
authors: Thomas
authors: T
authors: Noll
authors: Nanni
authors: N
authors: Jelinek
authors: Sebastian
authors: S
authors: Schmid
authors: Manfred
authors: M
authors: Biselli
authors: Christian
authors: C
authors: Wandrey
mesh_terms: D001672:Biocompatible Materials; D019149:Bioreactors; D018929:Cell Culture Techniques; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials