Detecting endocrine-disrupting compounds by fast impedance measurements.
The increasing concern worldwide over the adverse effects of endocrine disruptors on human health has created a need for screening systems to detect xenoestrogens, a diverse group of environmental chemicals that mimic estrogenic actions and are hypothesized to decrease male fertility. Here, we describe a novel, class-selective detector that uses fast impedance measurements to monitor the binding of estrogen and xenoestrogens to a native estrogen receptor. We embedded the receptor in synthetic lipid bilayers attached to gold electrodes. The lipid bilayers serve as electrical circuits constructed of resistors and capacitors. Estrogen binding to the receptor-modified electrode is immediately followed by conformational changes in the lipid layer, leading to alterations of the electrical circuit components that are detected by fast impedance measurements. The electrochemical system enabled characterization of changes in the bilayer structure and quantification of estrogen binding to the receptor. To assess the effectiveness of the method for detecting environmental estrogenic chemicals, we chose two classes of xenoestrogens: bisphenol A, a synthetic xenoestrogen, and genistein, a phytoestrogen. This system is highly sensitive and amenable to use in the field, providing an efficient and economic tool for measuring minuscule amounts of endocrine-disrupting chemicals in environmental or human samples.
Granek
Vered
V
Deapartment of Molecular Microbiology and Biotechnology, Tel-Aviv University, Ramat-Aviv, Israel.
Rishpon
Judith
J
D004563:Electrochemistry; D004566:Electrodes; D004784:Environmental Monitoring / Q000379:methods*; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D008055:Lipids; D008968:Molecular Conformation; D012680:Sensitivity and Specificity; D015262:Xenobiotics / Q000032:analysis*
D004968:Estrogens, Non-Steroidal; D008055:Lipids; D015262:Xenobiotics