title: Sin recombinase from Staphylococcus aureus: synaptic complex architecture and transposon targeting.
abstract: The Sin recombinase from Staphylococcus aureus builds a distinctive DNA-protein synaptic complex to regulate strand exchange. Sin binds at two sites within an 86 basepair (bp) recombination site, resH. We propose that inverted motifs at the crossover site, and tandem motifs at the regulatory site, are recognized by structurally disparate Sin dimers. An essential architectural protein, Hbsu, binds at a discrete central site in resH. Positions of Hbsu-induced DNA deformation coincide with natural targets for Tn552 integration. Remarkably, Sin has the same topological selectivity as Tn3 and gammadelta resolvases. Our model for the recombination synapse has at its core an assembly of four Sin dimers; Hbsu plays an architectural role that is taken by two resolvase dimers in models of the Tn3/gammadelta synapse.
authors: Institute of Biomedical and Life Sciences, University of Glasgow, Anderson College, UK. sr2q@udcf.gla.ac.uk
authors: Sally-J
authors: SJ
authors: Rowland
authors: W Marshall
authors: WM
authors: Stark
authors: Martin R
authors: MR
authors: Boocock
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000502:physiology*; D001483:Base Sequence; D001665:Binding Sites; D004254:DNA Nucleotidyltransferases / Q000737:chemistry / Q000502:physiology*; D004251:DNA Transposable Elements / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D004278:DNA, Superhelical / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D019281:Dimerization; D008957:Models, Genetic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D045522:Recombinases; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D013379:Substrate Specificity; D019895:Transposases / Q000737:chemistry / Q000378:metabolism; D045564:Transposon Resolvases
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; C075779:Hbsu protein, bacteria; D045522:Recombinases; D004254:DNA Nucleotidyltransferases; D019895:Transposases; D045564:Transposon Resolvases; C086893:sin protein, Staphylococcus aureus