Pentoxifylline reduces biochemical markers of ischemia-reperfusion induced spinal cord injury in rabbits.
METHODS
Occlusion of the infrarenal abdominal aorta with administration of pentoxifylline was applied to adult rabbits, followed by removal of aortic clamp and reperfusion. Tissue levels of cytokines, lipid peroxides, and antioxidant enzymes were assayed and compared within groups.


OBJECTIVE
To examine the effect of pentoxifylline (PTX) on cytokine levels, lipid peroxidation, and antioxidant enzymes in a rabbit model of spinal cord ischemia-reperfusion injury induced by aortic occlusion.


METHODS
Isparta, Turkey.


METHODS
Rabbits were randomly allocated into four groups of sham laparotomy (SHAM), sham laparotomy with PTX administration (SHAM+PTX), aortic occlusion and reperfusion (AOR), aortic occlusion and reperfusion with PTX administration (AOR+PTX). An intravenous bolus of 50 mg/kg PTX was given just before aortic cross clamping. An atraumatic microvascular clamp was then placed on the abdominal aorta immediately distal to the left renal artery for 30 min. PTX was infused at a rate of 0.5 mg/kg/min during the aortic occlusion. Animals were subjected to 120 min of reperfusion after removal of the aortic clamp. All animals were sacrificed at the end of reperfusion. The lumbosacral segments of spinal cords were quickly harvested and stored at -78 degrees C for biochemical assays of IL-6, TNF-alpha, MDA, SOD, and CAT levels. Differences among groups were analyzed by one-way analysis of variance followed by a post hoc Tukey's honestly significant difference test.


RESULTS
No differences in mean levels of IL-6, TNF-alpha, MDA, SOD, and CAT were noted between SHAM and SHAM+PTX groups (P>0.05). There was a significant increase in all biochemical parameters in the AOR group (P<0.05). Administration of PTX significantly attenuated the levels of all biochemical parameters in the AOR+PTX group (P<0.05).


CONCLUSIONS
PTX pretreatment attenuated ischemia-reperfusion induced spinal cord injury in a rabbit model, in terms of biochemical parameters of ischemia and reperfusion.
Savaş
S
S
Department of Physical Medicine and Rehabilitation, Suleyman Demirel University Medical School, Isparta, Turkey.
Delibaş
N
N
Savaş
C
C
Sütçü
R
R
Cindaş
A
A
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001018:Aortic Diseases / Q000150:complications; D001157:Arterial Occlusive Diseases / Q000150:complications; D015415:Biomarkers / Q000032:analysis; D016207:Cytokines / Q000187:drug effects* / Q000378:metabolism; D004195:Disease Models, Animal; D006401:Hematologic Agents / Q000494:pharmacology*; D007378:Interleukins / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010431:Pentoxifylline / Q000378:metabolism / Q000494:pharmacology*; D011817:Rabbits; D011897:Random Allocation; D015427:Reperfusion Injury / Q000209:etiology; D020760:Spinal Cord Ischemia / Q000097:blood* / Q000188:drug therapy* / Q000209:etiology; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000975:Antioxidants; D015415:Biomarkers; D016207:Cytokines; D006401:Hematologic Agents; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D010431:Pentoxifylline