[Expression and identification of specific autoantigens in autoimmune hepatitis].
OBJECTIVE
To express and identify soluble liver antigen (SLA) and cytochrome P-450 (CYP 2D6).


METHODS
SLA cDNA and CYP 2D6 cDNA were obtained from human liver tissue poly (A)+RNA by RT-PCR. The cDNAs were inserted into fusion expression vector PQE-30 site of BamH I and Hind III. SLA and CYP 2D6 were identified by the SDS-PAGE and Western blot.


RESULTS
SDS-PAGE analysis showed that there was a very strong stained band at about 47 kd and 50 kd, respectively. The products could specifically band to anti-SLA or anti-CYP 2D6 autoantibodies.


CONCLUSIONS
The clone and expression of SLA and CYP 2D6 provide useful substances for the diagnosis and research of pathogenesis on autoimmune hepatitis.
Fan
Lieying
L
Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Zhong
Renqian
R
Zhang
Shaojing
S
Wang
Hao
H
Gu
Jun
J
Zhu
Ye
Y
Han
Huanxing
H
D001324:Autoantigens / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D019693:Hepatitis, Autoimmune / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D001324:Autoantigens; D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; C046431:liver antigen LA-1; D003577:Cytochrome P-450 Enzyme System