Comparison of embryonated chicken eggs with MDCK cell culture for the isolation of swine influenza virus.
Embryonated chicken eggs (ECE) and the Madin-Darby canine kidney (MDCK) cell line were compared for isolation of swine influenza virus (SIV) from nasal swabs and tissue samples. Samples originated from 30 pigs experimentally inoculated with 2 x 106 to 2 x 10(7) embryo infectious dose 50% (EID50)/mL of swine influenza strain A/Swine/Indiana/1726/88 (H1N1). The results were analyzed with McNemar's chi-squared test for symmetry. The results indicated that more samples were SIV-positive with ECE than with tissue culture (P < 0.001), suggesting that ECE remains the system of choice for isolation of SIV. It is recommend that routine use of both SIV isolation systems will increase the sensitivity of detection of virus shedding by considering the differences in growth and tropism of diverse SIV strains.
Clavijo
Alfonso
A
National Centre for Foreign Animal Disease, Winnipeg, Manitoba. aclavijo@inspection.gc.ca
Tresnan
Dina B
DB
Jolie
Rika
R
Zhou
En-Min
EM
D000818:Animals; D002460:Cell Line / Q000821:virology; D002642:Chick Embryo / Q000821:virology*; D004531:Eggs / Q000821:virology; D009980:Influenza A virus / Q000302:isolation & purification*; D009296:Nasal Cavity / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology