Wild-type levels of Spo11-induced DSBs are required for normal single-strand resection during meiosis.
We have studied the repair of a DNA-DSB created by the VMA1-derived endonuclease in mutants that have different levels of Spo11-DSBs: WT (sae2), few (hop1), and none (spo11-Y135F). In spo11-Y135F and hop1 cells, intrachromosomal repair is more frequent than in WT and sae2 cells. In spo11-Y135F cells there was no chromosome pairing or synapsis and a faster turnover of resected DNA. Compared to WT and sae2 cells, spo11-Y135F and hop1 cells have a greater proportion of long resection tracts. The data suggest that high levels of Spo11-DSBs are required for normal regulation of resection, even at a DSB created by another protein. WT control over resection could be important for directing repair to be interchromosomal, increasing the chance of creating interhomolog connections essential to meiotic segregation.
Neale
Matthew J
MJ
Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, United Kingdom.
Ramachandran
Madhu
M
Trelles-Sticken
Edgar
E
Scherthan
Harry
H
Goldman
Alastair S H
AS
D019457:Chromosome Breakage; D015825:Chromosomes, Fungal / Q000235:genetics* / Q000648:ultrastructure; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000235:genetics* / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000502:physiology*; D004950:Esterases / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D008540:Meiosis / Q000235:genetics*; D008957:Models, Genetic; D006180:Proton-Translocating ATPases; D011995:Recombination, Genetic / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D017384:Sequence Deletion; D013573:Synaptonemal Complex / Q000502:physiology; D000099257:Meiotic Recombination Protein SPO11
D004271:DNA, Fungal; D004277:DNA, Single-Stranded; D004706:Endodeoxyribonucleases; D004950:Esterases; D006180:Proton-Translocating ATPases; D029701:Saccharomyces cerevisiae Proteins; D000099257:Meiotic Recombination Protein SPO11; C081687:VMA1 protein, S cerevisiae