Immunotherapy with autologous dendritic cells and tumor-specific synthetic peptides for synovial sarcoma.
Synovial sarcoma in an 11-year-old Japanese girl relapsed 5 months after autologous stem cell transplantation. Autologous dendritic cells (DCs) were generated from her peripheral blood mononuclear cells using granulocyte/macrophage colony-stimulating factor and IL-4. Dendritic cells were pulsed with synthetic peptides containing a junctional region of SYT-SSX2 fusion protein generated by t(X;18) and were administered once per week. No side effects were observed. Growth of metastatic nodules in the lung was temporally suppressed. The delayed-type hypersensitivity responses in skin were enhanced to tumor lysate but not to peripheral blood mononuclear cell lysate. The CD3+ cells cultured with pulsed DCs lysed tumor cells in vitro. Immunotherapy using DCs and tumor-specific peptides may be a safe approach in the treatment of childhood cancer.
Matsuzaki
Akinobu
A
Department of Pediatrics, Graduate School of Medical Sciences, Fukuoka, Japan. matsuzaki@shs.kyushu-u.ac.jp
Suminoe
Aiko
A
Hattori
Hiroyoshi
H
Hoshina
Takayuki
T
Hara
Toshiro
T
D000951:Antigens, Neoplasm / Q000276:immunology; D001859:Bone Neoplasms / Q000628:therapy*; D002478:Cells, Cultured / Q000166:cytology / Q000276:immunology; D002648:Child; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D017931:DNA Primers / Q000737:chemistry; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D007167:Immunotherapy; D015847:Interleukin-4 / Q000276:immunology; D009363:Neoplasm Proteins / Q000627:therapeutic use*; D009364:Neoplasm Recurrence, Local / Q000628:therapy*; D015514:Oncogene Proteins, Fusion / Q000627:therapeutic use*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D013902:Radiography, Thoracic; D013584:Sarcoma, Synovial / Q000628:therapy*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D013899:Thoracic Neoplasms / Q000628:therapy*
D000951:Antigens, Neoplasm; D017931:DNA Primers; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D010455:Peptides; C116512:SYT-SSX fusion protein; D015847:Interleukin-4