title: Repair of wounded monolayers of cultured bovine aortic endothelial cells is inhibited by calcium spirulan, a novel sulfated polysaccharide isolated from Spirulina platensis.
abstract: Calcium spirulan (Ca-SP) is a novel sulfated polysaccharide isolated from a blue-green alga Spirulina platensis. Ca-SP inhibits thrombin by activation of heparin cofactor II. Therefore, it could serve as an origin of anti-atherogenic medicines. Since maintenance of vascular endothelial cell monolayers is important for prevention of vascular lesions such as atherosclerosis, the effect of Ca-SP at 20 microg/ml or less on the repair of wounded bovine aortic endothelial cell monolayers in culture was investigated in the present study. When the monolayers were wounded and cultured in the presence of Ca-SP, the polysaccharide inhibited the appearance of the cells in the wounded area. The inhibition was also observed even when the repair was promoted by excess basic fibroblast growth factor, which is one of the autocrine growth factors that are involved in the endothelial cell monolayer maintenance. On the other hand, Ca-SP inhibited the cell growth and the incorporation of [3H]thymidine into the acid-insoluble fraction of proliferating endothelial cells, suggesting that Ca-SP inhibits endothelial cell proliferation. From these results, it is concluded that Ca-SP may retard the repair process of damaged vascular endothelium through inhibition of vascular endothelial cell proliferation by induction of a lower ability to respond to stimulation by endogenous basic fibroblast growth factor.
authors: Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan. t-kaji@hokuriku-u.ac.jp
authors: Toshiyuki
authors: T
authors: Kaji
authors: Yasuyuki
authors: Y
authors: Fujiwara
authors: Yuki
authors: Y
authors: Inomata
authors: Chieko
authors: C
authors: Hamada
authors: Chika
authors: C
authors: Yamamoto
authors: Satomi
authors: S
authors: Shimada
authors: Jung-Bum
authors: JB
authors: Lee
authors: Toshimitsu
authors: T
authors: Hayashi
mesh_terms: D000818:Animals; D001161:Arteriosclerosis / Q000517:prevention & control*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D011134:Polysaccharides; D016222:Fibroblast Growth Factor 2; C102617:spirulan