High-density fiber-optic genosensor microsphere array capable of zeptomole detection limits.
The detection limit of a fiber-optic microsensor array was investigated for simultaneous detection of multiple DNA sequences. A random array composed of oligonucleotide-functionalized 3.1-microm-diameter microspheres on the distal face of a 500-microm etched imaging fiber was monitored for binding to fluorescently labeled complementary DNA sequences. Inherent sensor redundancy in the microarray allows the use of multiple microspheres to increase the signal-to-noise ratio, further enhancing the detection capabilities. Specific hybridization was observed for each of three sequences in an array yielding a detection limit of 10(-21) mol (approximately 600 DNA molecules).
Epstein
Jason R
JR
The Max Tishler Laboratory for Organic Chemistry, Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
Lee
Myoyong
M
Walt
David R
DR
D000818:Animals; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry; D008297:Male; D008863:Microspheres; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D012474:Salmon; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004247:DNA