Proton nuclear magnetic resonance characterisation of glycosaminoglycans using chemometric techniques.
Various types of glycosaminoglycans (GAGs) including heparins, chondroitin sulfates, dermatan sulfate and hyaluronic acid were studied from their proton nuclear magnetic resonance (1H NMR) spectra using chemometric techniques. Despite the complexity of the 1H NMR signals, data analysis using principal component analysis enabled the different GAG classes to be distinguished and permitted their classification according to their chemical structure. The analysis of the composition of the major disaccharide unit and other relevant chemical structures in the heparin samples was performed using partial least squares regression.
Ruiz-Calero
Victoria
V
Department of Analytical Chemistry, University of Barcelona, Spain.
Saurina
Javier
J
Hernández-Cassou
Santiago
S
Galceran
M Teresa
MT
Puignou
Lluis
L
D019075:Electrophoresis, Capillary; D006025:Glycosaminoglycans / Q000032:analysis*; D019906:Nuclear Magnetic Resonance, Biomolecular; D012815:Signal Processing, Computer-Assisted
D006025:Glycosaminoglycans