[Expression of integrin alpha 6 in hepatic sinusoidal capillaration].
OBJECTIVE
To explore the expression of integrin alpha 6 in hepatic sinusoidal capillaration.


METHODS
The rat hepatic fibrosis model was established by injection of carbon tetrachloride subcutaneously. Then the expression of laminin and integrin alpha 6 subunit was observed by immunohistochemistry and dot immuno-blotting.


RESULTS
We observed sinusoidal capillaration formed by deposition of laminin along sinusoids in Disse interspace by immunohistochemistry staining. In normal rat the expression of integrin alpha 6 was restricted to portal vascular endothelial cells and bile duct epithelial cell membranes. No expression was observed in sinusoidal endothelial cell membranes. When capillaration integrin alpha 6 was detected in a continuous pattern along the sinusoids, the content of integrin alpha 6 was significantly higher in fibrotic liver tissues than in normal liver tissues as measured by dot immuno-blotting (P<0.05).


CONCLUSIONS
During fibrogenesis, laminin continuously accumulate in liver tissues and form basement membrane resulting in sinusoidal capillaration, and then induce the expression of integrin alpha 6 on SEC membranes.
Xiao
Wenjun
W
Digestive Medicine Institute of Huaxi Hospital of Sichuan University, Chengdu 610041, China.
Wang
Yiping
Y
Liu
Xiaojing
X
Huang
Minghui
M
Gan
Tao
T
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis*; D002251:Carbon Tetrachloride; D015151:Immunoblotting; D007150:Immunohistochemistry; D039503:Integrin alpha6; D007797:Laminin / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D015703:Antigens, CD; D039503:Integrin alpha6; D007797:Laminin; D002251:Carbon Tetrachloride