Spontaneous release of T cell receptors for alloantigens. III. The effects of anti-T cell receptor antiserum, of alloantiserum and of trypsin on T cell receptor release.
Spleen and lymph node T lymphocytes cultivated in vitro spontaneously released receptors for alloantigens. Among the various specificities, those receptors fitting certain alloantigens could be absorbed by formolized cells or proper genotype without influencing receptors for other alloantigens. Shedding of T cell receptors could be inhibited for 8 h by treating cells with antisera directed against T cell receptors (anti-RS antisera). Inhibition experiments indicated that resynthesis of T cell receptors appears to be complete by 8 h. Experiments on prevention of receptor shedding revealed highly specific and presumably cytotoxic elimination of T cells with receptors for given alloantigens. Treatment of cells with alloantiserum obtained by skin graft rejection failed to inhibit spontaneous release of T cell receptors, whereas treatment of lymphocytes with trypsin resulted in an 8-h delay of receptor shedding similar to that after treatment of cells with anti-RS antisera. This inhibition was, however, nonspecific.
Ramseier
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Division of Experimental Microbiology, Department of Medical Microbiology, University of Zürich, Gloriastrasse 32, CH-8028 Zürich, Switzerland.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D006084:Graft Rejection / Q000276:immunology; D066298:In Vitro Techniques; D007518:Isoantibodies / Q000494:pharmacology; D007519:Isoantigens / Q000378:metabolism*; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D016038:Skin Transplantation / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014357:Trypsin / Q000494:pharmacology
D000961:Antilymphocyte Serum; D007518:Isoantibodies; D007519:Isoantigens; D011948:Receptors, Antigen, T-Cell; D014357:Trypsin