Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome.
In signal transduction in eukaryotes, protein phosphorylation is a key event. To understand signaling processes, we must first acquire an inventory of phosphoproteins and their phosphorylation sites under different conditions. Because phosphorylation is a dynamic process, elucidation of signaling networks also requires quantitation of these phosphorylation events. In this article, we outline several methods for enrichment of phosphorylated proteins and peptides and discuss various options for their identification and quantitation with special emphasis on mass spectrometry-based techniques.
Mann
Matthias
M
Center for Experimental Bioinformatics, University of Southern Denmark, M, DK-5230, Odense, Denmark. mann@bmb.sdu.dk
Ong
Shao En
SE
Grønborg
Mads
M
Steen
Hanno
H
Jensen
Ole N
ON
Pandey
Akhilesh
A
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002845:Chromatography / Q000379:methods; D005583:Fourier Analysis; D013058:Mass Spectrometry / Q000379:methods*; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D010766:Phosphorylation; D020543:Proteome / Q000737:chemistry*; D015398:Signal Transduction
D000906:Antibodies; D010750:Phosphoproteins; D020543:Proteome