Effect of gamma-irradiation on H3 histone and DNA in solution.
Three methods, namely, absorbance of colour by reaction with Folin-Ciocalteau reagent, UV absorbance and fluorescence intensity measurements for detection of H3 histone in 0.15 M standard saline citrate (SSC) solution were compared. Maximum sensitivity was found with the Folin-Ciocalteau method. Effect of varying pH and of gamma- radiation on H3 histone and on interaction of H3 histone with DNA were studied. For this, solutions of H3 histone in SSC, in 0.9% NaCl, H3 histone + DNA in 0.9% NaCl were subjected to varying pH (1-10) and gamma- radiation (dose 10-50 Gy) and lambda(max) and Alambda(max) were monitored. From the molar ratios of histone and DNA in the complex, it was observed that at gamma -radiation dose of 50 Gy and pH 8.54, there was a depletion of 6-8 microg/ml of histone from the histone-DNA complex.
Upadhyay
S N
SN
Radiation Biology Department, Institute of Nuclear Medicine & Allied Sciences, Delhi.
D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism / Q000528:radiation effects*; D005720:Gamma Rays; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism / Q000528:radiation effects*; D008982:Molybdenum; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013950:Thymus Gland / Q000737:chemistry; D017973:Tungsten Compounds
C029556:Folin's phenol reagent; D006657:Histones; D017973:Tungsten Compounds; D008982:Molybdenum; D004247:DNA