RP-LC determination of oleane derivatives in Terminalia arjuna.
A rapid sensitive and reproductive reversed phase high performance liquid chromatographic method with photo diode arrray detection is described for the simultaneous quantification of major oleane derivatives: arjunic acid (4), arjunolic acid (3), arjungenin (2) and arjunetin (1) in Terminalia arjuna extract. The method involves the use of a Waters Spherisorb S10 ODS2 column (250 x 4.6 mm, I.D., 10 microm) and binary gradient mobile phase profile. The various other aspects of analysis viz. Extraction efficiency, peak purity and similarity were validated using a photo diode array detector.
Singh
D V
DV
Analytical Biophysical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O.-CIMAP, Lucknow 226 015, India.
Verma
R K
RK
Singh
S C
SC
Gupta
M M
MM
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D007194:India; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000032:analysis; D019441:Plant Epidermis / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents
D012997:Solvents; D009828:Oleanolic Acid