title: Mycobacterium simiae infection in a patient with acquired immunodeficiency syndrome.
abstract: Mycobacterium simiae is usually an environmental contaminant rarely associated with human disease. We report a fatal case of M.simiae infection in a 37 year old, HIV positive, male from whom the organism was isolated from blood culture. The identification of M.simiae was performed using DNA amplification followed by analysis on 3% agarose gel of the amplicon fragments after digestion by restriction endonucleases. The precise identification of mycobacterial isolates to the species level is important, with both epidemiological and therapeutic implications.
authors: Lamina Laboratory, Rio de Janeiro, RJ, Brazil. jorge.sampaio@fleury.com.br
authors: J L
authors: JL
authors: Sampaio
authors: N
authors: N
authors: Artiles
authors: R M
authors: RM
authors: Pereira
authors: J R
authors: JR
authors: Souza
authors: J P
authors: JP
authors: Leite
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology*; D000328:Adult; D017809:Fatal Outcome; D006801:Humans; D008297:Male; D009161:Mycobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length