title: Suicide inactivation of peroxidases and the challenge of engineering more robust enzymes.
abstract: As the number of industrial applications for proteins continues to expand, the exploitation of protein engineering becomes critical. It is predicted that protein engineering can generate enzymes with new catalytic properties and create desirable, high-value, products at lower production costs. Peroxidases are ubiquitous enzymes that catalyze a variety of oxygen-transfer reactions and are thus potentially useful for industrial and biomedical applications. However, peroxidases are unstable and are readily inactivated by their substrate, hydrogen peroxide. Researchers rely on the powerful tools of molecular biology to improve the stability of these enzymes, either by protecting residues sensitive to oxidation or by devising more efficient intramolecular pathways for free-radical allocation. Here, we discuss the catalytic cycle of peroxidases and the mechanism of the suicide inactivation process to establish a broad knowledge base for future rational protein engineering.
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, AP 510-3 Cuernavaca, Morelos 62250, México. brenda@ibt.unam.mx
authors.forename: Brenda
authors.initials: B
authors.lastname: Valderrama
authors.forename: Marcela
authors.initials: M
authors.lastname: Ayala
authors.forename: Rafael
authors.initials: R
authors.lastname: Vazquez-Duhalt
mesh_terms: D002384:Catalysis; D030562:Databases, Protein; D004795:Enzyme Stability; D005609:Free Radicals / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010544:Peroxidases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*
chemical_list: D005609:Free Radicals; D006861:Hydrogen Peroxide; D010544:Peroxidases; D010100:Oxygen