title: Postnatal expression and distribution of Refsum disease gene associated protein in the rat retina and visual cortex: effect of binocular visual deprivation.
abstract: Previously, phytanoyl-CoA alpha-hydroxylase-associated protein 1 (PAHX-AP1) was isolated as a novel neuron-specific protein to interact with Refsum disease (RfD) gene PAHX. Its expression in the brain increased after eyelid opening, and the elevated level was maintained through adulthood. In this report, to verify the hypothesis that light could trigger this increase, we have examined the developmental distribution pattern of PAHX-AP1 in rat retina and visual cortex, and changes of its expression by binocular deprivation. Northern blot analyses demonstrated PAHX-AP1 expression reached its highest level in the visual cortex and eyeball at 4 weeks after birth, and these levels were maintained through adult life. Two weeks after visual deprivation, its expression in the eyeball and visual cortex decreased compared with the control. In situ hybridization analyses of the retina showed that PAHX-AP1 expression was limited to the ganglionic cell layer at 10 days after birth, but expressed in the inner nuclear cell layer and extended to the outer nuclear cell layer at 2 and 3 weeks after birth, respectively. Two weeks after visual deprivation, however, it decreased in the ganglionic and inner nuclear cell layer, and disappeared in the rod and cone cell layers. In the visual cortex, strong signals of PAHX-AP1 were detected in layers IV and VI, and II-VI at 10 days and 2 weeks after birth, respectively. Its expression decreased after 2 weeks of visual deprivation. These results indicate that visual stimulation is essential for the maintenance of PAHX-AP1 expressions in the retina, especially in the rod and cone cell layers, and visual cortex, and suggest that PAHX-AP1 may be involved in the developmental regulation of the photoreceptor's function.
authors.affiliation: Research Institute of Medical Sciences, Chonnam National University Medical School, Hak-Dong 5, Dong-Ku, Kwangju 501-190, South Korea.
authors.forename: Kyu Youn
authors.initials: KY
authors.lastname: Ahn
authors.forename: Kwang Il
authors.initials: KI
authors.lastname: Nam
authors.forename: Baik Yoon
authors.initials: BY
authors.lastname: Kim
authors.forename: Chul Woong
authors.initials: CW
authors.lastname: Cho
authors.forename: Sang Ki
authors.initials: SK
authors.lastname: Jeong
authors.forename: Kun Jin
authors.initials: KJ
authors.lastname: Yang
authors.forename: Kyung Keun
authors.initials: KK
authors.lastname: Kim
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012035:Refsum Disease / Q000235:genetics; D012160:Retina / Q000737:chemistry* / Q000254:growth & development*; D012683:Sensory Deprivation; D014785:Vision, Ocular; D014793:Visual Cortex / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C429045:Phyhip protein, mouse; D012333:RNA, Messenger