Immunolocalization of 1- O-sinapoylglucose:malate sinapoyltransferase in Arabidopsis thaliana.
The serine carboxypeptidase-like protein 1- O-sinapoylglucose:malate sinapoyltransferase (SMT) catalyzes the transfer of the sinapoyl moiety of 1- O-sinapoylglucose to malate in the formation of sinapoylmalate in some members of the Brassicaceae. Rabbit polyclonal monospecific antibodies were raised against the recombinant SMT produced in Escherichia coli from the corresponding Arabidopsis thaliana (L.) Heynh. cDNA. Immunoblot analysis of protein from different Arabidopsis tissues showed that the SMT is produced in all plant organs, except in the seeds and young seedlings. The enzyme was most abundant in older seedlings as well as in rosette leaves and the flowering stem of the plant. Minor amounts were found in the cauline leaves, flower buds and siliques. Traces were detected in the root and flowers. Arabidopsis and transgenic tobacco ( Nicotiana tabacum L.) plants expressing the full-length Arabidopsis SMT containing an N-terminal signal peptide showed apparent molecular masses of the protein of 52-55 kDa. The difference of ca. 8 kDa compared to the recombinant protein produced in E. coli was shown to be due to post-translational N-glycosylation of SMT in plants. Immunofluorescent labeling of Arabidopsis leaf sections localized SMT to the central vacuoles of mesophyll and epidermal cells. Comparable leaf sections of an SMT deletion mutant showed no vacuolar immunofluorescent labeling. We conclude that Arabidopsis SMT is synthesized as a precursor protein that is targeted to the endoplasmic reticulum where the signal peptide is removed. The correct N-terminus of the recombinantly produced SMT protein lacking the signal peptide was confirmed by Edman degradation. The protein is probably glycosylated in the Golgi apparatus from where it is subsequently routed to the vacuole.
Hause
Bettina
B
Institut für Pflanzenbiochemie, Abteilung Sekundärstoffwechsel, Weinberg 3, 06120 Halle (Saale), Germany.
Meyer
Knut
K
Viitanen
Paul V
PV
Chapple
Clint
C
Strack
Dieter
D
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D015153:Blotting, Western; D002934:Cinnamates / Q000378:metabolism; D005960:Glucosides / Q000378:metabolism; D007150:Immunohistochemistry; D008293:Malates / Q000138:chemical synthesis / Q000378:metabolism; D015394:Molecular Structure; D010666:Phenylpropionates / Q000138:chemical synthesis; D018515:Plant Leaves / Q000201:enzymology; D018547:Plant Stems / Q000201:enzymology; D030821:Plants, Genetically Modified; D011817:Rabbits; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics
C079164:2-O-sinapoylmalate; D002934:Cinnamates; D005960:Glucosides; D008293:Malates; D010666:Phenylpropionates; C103373:sinapoylglucose; C030298:malic acid; D000217:Acyltransferases; C416511:sinapoylglucose malate sinapoyltransferase