Hadamard transform time-of-flight mass spectrometry: a high-speed detector for capillary-format separations.
This work demonstrates that with an intrinsic duty cycle of 50% and spectral storage speeds up to 277 spectra s(-1) Hadamard transform time-of-flight mass spectrometry (HT-TOFMS) is a promising detector for any capillary-format separation that can be coupled to MS by electrospray ionization. Complete resolution of the components of a nine-peptide standard was achieved by coupling pressurized-capillary electrophoresis (pCE) to HT-TOFMS. The addition of pressure to the separation capillary decreased analysis times and stabilized the electrospray ionization source. Pulsed-pressurized injection of reserpine was used to experimentally simulate narrower peaks than those obtained in the pCE. HT-TOFMS was able to sample peaks having widths in the millisecond range.
Fernández
Facundo M
FM
Department of Chemistry, Stanford University, California 94305-5080, USA.
Vadillo
José M
JM
Kimmel
Joel R
JR
Wetterhall
Magnus
M
Markides
Karin
K
Rodriguez
Nestor
N
Zare
Richard N
RN
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods / Q000592:standards; D016247:Information Storage and Retrieval; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D013997:Time Factors
D010455:Peptides