Lentivirus lytic peptide 1 perturbs both outer and inner membranes of Serratia marcescens.
Bis-lentivirus lytic protein 1 (Bis-LLP1) and polymyxin B exhibited similar killing activities against Serratia marcescens. By electron microscopy, bis-LLP1 interacted with the outer and cytoplasmic bacterial membranes, while polymyxin B affected only the outer membrane. The results of standard biochemical probes supported the findings of the electron microscopy studies, suggesting that these antimicrobial peptides have different mechanisms of action.
Phadke
Shruti M
SM
Department of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA.
Lazarevic
Vanja
V
Bahr
Caroline C
CC
Islam
Kazi
K
Stolz
Donna Beer
DB
Watkins
Simon
S
Tencza
Sarah B
SB
Vogel
Hans J
HJ
Montelaro
Ronald C
RC
Mietzner
Timothy A
TA
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure; D005614:Freeze Fracturing; D008565:Membrane Proteins / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D011112:Polymyxin B / Q000494:pharmacology; D012706:Serratia marcescens / Q000187:drug effects* / Q000648:ultrastructure
D000900:Anti-Bacterial Agents; D008565:Membrane Proteins; D011112:Polymyxin B