[The analysis of biological characteristics for the tissue-plasminogen activator (t-PA) mutant engineered cell line].
The morphology of t-PA mutant engineered cell line FSGGI48 is similar to that of CHO-dhfr- cell in polygon. When aminopterin (MTX) concentration is 5 x 10(-6) mol/L, a part of cells become slim, but they are still in polygon. Therefore, the morphology of FSGGI48 cell line is normal. The expression level is 4000 IU/10(6) cells/24 h in serum-free medium. The cell line is bequeathed for three months in vitro and revived after freezing three months, the greater part of cells are stable. The expression level is 3000-4000 IU/10(6) cells/24 h. The assay of tumorigenesis shows that the cells, cellular DNA and purified products don't form tumors in nude mice. The cell line hasn't been contaminated by mycoplasma. The results of chromosomal analysis show that the chromosomal number of CHO-dhfr- cell is 20 and the abnormal rate is 6%. FSGGI48 cell line is same as CHO-dhfr- cell in chromosomal number and the abnormal rate is 15%-18%, it is normal in scope. Therefore, the engineered cell line FSGGI48 is excellent.
Chen
L
L
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071.
Xu
X Y
XY
Liu
S H
SH
Zhu
H Q
HQ
Huang
P T
PT
Huang
C F
CF
D000818:Animals; D016466:CHO Cells / Q000378:metabolism*; D015197:Carcinogenicity Tests; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009154:Mutation; D013762:Tetrahydrofolate Dehydrogenase / Q000172:deficiency; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics*
D013762:Tetrahydrofolate Dehydrogenase; D010959:Tissue Plasminogen Activator