title: Cloning of a tobacco cDNA coding for a putative transcriptional coactivator MBF1 that interacts with the tomato mosaic virus movement protein.
abstract: Viral movement through plasmodesmata in host plants depends on the interaction between virus-encoded movement protein (MP) and host proteins. To search for MP-interacting protein (MIP), far-western screening of a tobacco cDNA library was carried out using a recombinant MP of tomato mosaic virus (ToMV) as a probe. One of the positive cDNA clones, designated MIP204, was highly homologous to a class of transcriptional coactivators commonly referred to as multiprotein bridging factor 1 (MBF1). ToMV MP could also bind to the Arabidopsis homologues of MBF1. The recombinant MIP204 bound to MPs of ToMV and a crucifer tobamovirus CTMV-W, but not of cucumber mosaic virus. MPs of ToMV and the related virus may interact with MBF1-like proteins to modulate host gene expression.
authors.affiliation: Gene Research Center, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Matsushita
authors.forename: Orie
authors.initials: O
authors.lastname: Miyakawa
authors.forename: Masakazu
authors.initials: M
authors.lastname: Deguchi
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nishiguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nyunoya
mesh_terms: D029681:Arabidopsis Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D053830:Plant Viral Movement Proteins; D011485:Protein Binding; D014026:Nicotiana / Q000235:genetics*; D014027:Tobacco Mosaic Virus / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; C491466:MBF1 protein, Arabidopsis; D053830:Plant Viral Movement Proteins; D015534:Trans-Activators; D014764:Viral Proteins