title: Identification and characterization of GSTT3, a third murine Theta class glutathione transferase.
abstract: A novel Theta class glutathione transferase (GST) isoenzyme from mouse termed mGSTT3 has been identified by analysis of the expressed sequence tag database. The gene encoding mGSTT3 is clustered with the mGSTT1 and mGSTT2 genes on chromosome 10 and has an exon/intron structure that is similar to that of the other Theta class genes. mGSTT3 is expressed strongly in the liver and to a decreasing extent in the kidney and testis. Recombinant mGSTT3-3 expressed in Escherichia coli had a substrate-specificity profile that differed significantly from that of GSTT1-1 and GSTT2-2 isoenzymes. A molecular model of mGSTT3 suggested that, in comparison with GSTT2, a decrease in volume of the hydrophobic substrate-binding site and the loss of the sulphate-binding pocket prevents its use of the GSTT2 substrate 1-menaphthyl sulphate.
authors.affiliation: Molecular Genetics Group, John Curtin School of Medical Research, Australian National University, P.O. Box 334, Canberra, Australian Capital Territory, 2601, Australia.
authors.forename: Marjorie
authors.initials: M
authors.lastname: Coggan
authors.forename: Jack U
authors.initials: JU
authors.lastname: Flanagan
authors.forename: Michael W
authors.initials: MW
authors.lastname: Parker
authors.forename: Vanicha
authors.initials: V
authors.lastname: Vichai
authors.forename: William R
authors.initials: WR
authors.lastname: Pearson
authors.forename: Philip G
authors.initials: PG
authors.lastname: Board
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D019992:Databases as Topic; D004926:Escherichia coli / Q000201:enzymology; D005091:Exons; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007438:Introns; D051379:Mice; D008957:Models, Genetic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014018:Tissue Distribution
chemical_list: D011994:Recombinant Proteins; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase