Alternative splicing, expression, and gene structure of the septin-like putative proto-oncogene Sint1.
Sint1 (sept9), a murine gene of the septin family, was previously isolated as a putative proto-oncogene involved in T-cell lymphomagenesis. We now present its genomic structure and report on nine exons shared by all identified variants and at least four alternatively spliced 5' exons. Northern blot analyses using a Sint1 cDNA probe showed in almost all examined tissues two predominant transcripts of 3 and 4 kb. Exon-specific expression analyses assigned one of the 5' exons to the 4 kb transcript, while the other 5' exons seem to represent novel, tissue-specific, weakly expressed transcripts of different sizes, and none of them appear to hybridize to the major 3 kb transcript. Whole-mount in situ hybridization on post-implantation embryos revealed several areas strongly expressing Sint1, including neural crest cells, cephalic mesenchyme, and mesenchymal cells in the developing limb. A clustering of proviruses in four independent retrovirally induced tumors point to a region of about 3 kb around the most upstream exon as important for proviral deregulation of Sint1.
Sørensen
Annette Balle
AB
Department of Molecular and Structural Biology, University of Aarhus, DK-8000, Aarhus C, Denmark.
Warming
Søren
S
Füchtbauer
Ernst Martin
EM
Pedersen
Finn Skou
FS
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004622:Embryo, Mammalian / Q000378:metabolism; D047108:Embryonic Development; D005091:Exons; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D005796:Genes / Q000235:genetics; D005813:Genes, Synthetic; D017403:In Situ Hybridization; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D011247:Pregnancy; D011519:Proto-Oncogenes / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004268:DNA-Binding Proteins; D016601:RNA-Binding Proteins; C116974:sint1 protein, synthetic; D004247:DNA