title: An integrated comparative map of the porcine X chromosome.
abstract: The objectives of this study were to assign both microsatellite and gene-based markers on porcine chromosome X to two radiation hybrid (RH) panels and to develop a more extensive integrated map of SSC-X. Thirty-five microsatellite and 20 gene-based markers were assigned to T43RH, and 16 previously unreported microsatellite and 15 gene-based markers were added to IMpRH map. Of these, 30 microsatellite and 12 gene-based markers were common to both RH maps. Twenty-two gene-based markers were submitted to BLASTN analysis for identification of orthologues of genes on HSA-X. Single nucleotide polymorphisms (SNPs) were detected for 12 gene-based markers, and nine of these were placed on the genetic map. A total of 92 known loci are present on at least one porcine chromosome X map. Thirty-seven loci are present on all three maps; 31 loci are found on only one map. Location of 33 gene-based markers on the comprehensive map translates into an integrated comparative map that supports conservation of gene order between SSC-X and HSA-X. This integrated map will be valuable for selection of candidate genes for porcine quantitative trait loci (QTLs) that map to SSC-X.
authors.affiliation: USDA-ARS US Meat Animal Research Center, Clay Center, NE 68933-0166, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: McCoard
authors.forename: S C
authors.initials: SC
authors.lastname: Fahrenkrug
authors.forename: L J
authors.initials: LJ
authors.lastname: Alexander
authors.forename: B A
authors.initials: BA
authors.lastname: Freking
authors.forename: G A
authors.initials: GA
authors.lastname: Rohrer
authors.forename: T H
authors.initials: TH
authors.lastname: Wise
authors.forename: J J
authors.initials: JJ
authors.lastname: Ford
mesh_terms: D000818:Animals; D005819:Genetic Markers; D016678:Genome; D006801:Humans; D021101:Radiation Hybrid Mapping; D013552:Swine / Q000235:genetics*; D014960:X Chromosome
chemical_list: D005819:Genetic Markers