title: [CaSRB9, a novel Candida albicans gene, plays a role in morphogenesis of Saccharomyces cerevisiae].
abstract: Candida albicans is the most frequently isolated fungal pathogen in humans. Many factors are involved in its morphological transition. Flo8 plays an important role in morphogenesis of Saccharomyces cerevisiae. In this work, a C.albicans genomic DNA library was introduced into an S.cerevisiae flo8/flo8 mutant to screen genes which could complement its invasive growth defect. In this screening, a novel gene was isolated and designated CaSRB9 (Candida albicans SRB9 gene). The CaSRB9 gene had an ORF of 4 998 bp, encoding a putative protein of 1 665 amino acids. The CaSrb9 shared highest similarity in amino acids (38%) with Srb9 of S.cerevisiae. Ectopic expression of the CaSRB9 gene in diploid S. cerevisiae suppressed defect in filamentous growth of some mutants in filamentation MAPK pathway (ste7/ste7, ste 12 / ste 12, and tec 1 / tec 1) and flo 8 / flo 8 mutant under nitrogen starvation conditions. In haploid S. cerevisiae, ectopic expressed CaSrb9 complemented the invasive growth defect of flo8 mutant but failed to complement the invasive growth defects of the mutants in filamentation MAPK pathway.
authors.affiliation: State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhou
authors.forename: F
authors.initials: F
authors.lastname: Cao
authors.forename: J Y
authors.initials: JY
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D002176:Candida albicans / Q000235:genetics*; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D056892:Mediator Complex; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics* / Q000494:pharmacology
chemical_list: C100940:FLO8 protein, S cerevisaie; D005656:Fungal Proteins; D056892:Mediator Complex; D009687:Nuclear Proteins; C093967:SSN2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors