Autoantibodies against golgi apparatus induced by arteriviruses.
Members of the genus Arterivirus within the monogeneric family Arteriviridae are lactate dehydrogenase-elvating virus (LDV), porcine reproductive and respiratory syndrome virus (PRRSV), equine arteritis virus (EAV) and simian hemorrhagic fever virus. In LDV-infected mice the appearance of autoantibodies against Golgi-antigen dominated the early immune response. Shared antigenicity between LDV and Golgi-antigen of normal cells could not be demonstrated. Monoclonal antibodies (MAbs) reacted either with LDV or with Golgi-antigen but not with both. Immunization of mice with the porcine arterivirus PRRSV, however, led to the establishment of MAbs that recognized the structural glycoprotein GP3 as well as Golgi-antigen of normal porcine cells indicating molecular mimicry of viral and cellular antigen. In addition to cross-reactive antibodies MAbs solely reactive with Golgi-antigen were observed. After immunization of mice with EAV, the equine arterivirus, clones were isolated producing Golgi-antigen recognizing autoantibodies. Morphogenesis of arteriviruses occurs in the Golgi region. The autoimmune responses following immunization with arteriviruses may offer an approach for determining the mechanism by which such responses develop and become of biologic importance.
Weiland
Emilie
E
Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany. E.Weiland@tue.bfav.de
Weiland
Frank
F
D016475:3T3 Cells; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D018018:Arterivirus / Q000276:immunology* / Q000648:ultrastructure; D001323:Autoantibodies / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D007772:Lactate dehydrogenase-elevating virus / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; C468294:GP3 protein, equine arteritis virus; D014759:Viral Envelope Proteins