Expansion of megakaryocyte progenitors from cryopreserved leukocyte concentrates of human placental and umbilical cord blood in short-term liquid culture.
Long-term severe thrombocytopenia following human placental and umbilical cord blood (CB) transplantation is a significant clinical problem. We studied the ex vivo expansion of megakaryocytic progenitor cells (CFU-Meg) from cryopreserved/thawed leukocyte concentrates (LC) of CB prepared by the Tokyo Cord Blood Bank protocol. The LC cells were cultured in serum-free culture medium supplemented with a combination of early-acting cytokines including thrombopoietin (TPO), flt3-ligand (FL), and stem cell factor (SCF). Combination of TPO plus FL, TPO plus SCF, and all of these cytokines together resulted in 8.9-, 7.7-, and 8.4-fold increases in CFU-Meg, respectively, by Day 5 of culture. Our results showed that this simple expansion strategy has the potential for expanding CFU-Meg from cryopreserved/thawed LC cells from CB.
Sasayama
N
N
Cell Processing Department, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan.
Kashiwakura
I
I
Tokushima
Y
Y
Wada
S
S
Murakami
M
M
Hayase
Y
Y
Takagi
Y
Y
Takahashi
T A
TA
D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage; D015925:Cryopreservation; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007962:Leukocytes / Q000166:cytology* / Q000502:physiology; D008533:Megakaryocytes / Q000166:cytology* / Q000502:physiology; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D013997:Time Factors