Haptens and monoclonal antibodies for immunoassay of imidazolinone herbicides.
A set of haptens structurally resembling the herbicide imazethapyr (PURSUIT) was synthesized and used to derive monoclonal antibodies (MAbs) and direct and indirect competition enzyme immunoassays (EIAs) which could detect imazethapyr, imazaquin (SCEPTER), imazapic (CADRE), and imazamox (RAPTOR) in the 3-30 ng/mL (parts per billion) range, and imazapyr (ARSENAL) and imazamethabenz-methyl (ASSERT) in the 300-500 ppb range. Two MAbs, 3A2 and 3A5, had affinities of 10-75 nM for imazethapyr. MAbs 1A5, 1D2, and 3A5 were specific for the S isomers of the herbicides. Some MAbs were stable in solutions containing up to 15% methanol and 5% acetonitrile in indirect EIAs. Plates coated with hapten conjugates for indirect EIA could be stored frozen. Selectivity for the imidazolinones by some MAbs varied with different coating conjugates. These MAbs and haptens should prove useful in immunochemical analysis and residue recovery methods for imazethapyr and other imidazolinone herbicides.
Chin
Tina E
TE
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720-3206, USA. tina@molsci.org
Wong
Rosie B
RB
Pont
Joseph L
JL
Karu
Alexander E
AE
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D005260:Female; D006241:Haptens; D006540:Herbicides / Q000032:analysis*; D006825:Hybridomas / Q000276:immunology; D007093:Imidazoles / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D009539:Nicotinic Acids / Q000032:analysis / Q000276:immunology; D012996:Solutions
D000911:Antibodies, Monoclonal; D006241:Haptens; D006540:Herbicides; D007093:Imidazoles; D009539:Nicotinic Acids; D012996:Solutions; C093630:imazethapyr