title: [Study on gene expression changes of arsenic sulfide treated NB4 cells].
abstract: OBJECTIVE
  To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.
  
  
  METHODS
  Two cDNA probes were made from mRNA of untreated or arsenic sulfide treated NB4 cells. The cells were labelled with Cy3 or Cy5 fluorescence dyes individually, hybridized with cDNA microarray, and scanned for fluorescent intensity. The altered gene expression was screened through the analysis of difference in gene expression profile.
  
  
  RESULTS
  Thirty four genes related to apoptosis, cell cycle and others expressed different after the treatment of arsenic sulfide, 28/34 were up-regulated, 6/34 down-regulated.
  
  
  CONCLUSIONS
  ABC50, PNAS-2 and cyclin G(2) might take part in the process of NB4 cell apoptosis induced by arsenic sulfide.
authors.affiliation: Department of Hematology, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Gu
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Chen
authors.forename: Jieying
authors.initials: J
authors.lastname: Han
authors.forename: Ye
authors.initials: Y
authors.lastname: Teng
authors.forename: Nianxian
authors.initials: N
authors.lastname: Shao
authors.forename: Renrong
authors.initials: R
authors.lastname: Ouyang
mesh_terms: D001152:Arsenicals / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D013440:Sulfides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
chemical_list: D001152:Arsenicals; D013440:Sulfides; C045816:arsenic trisulfide