[Increased expression of monocytic cell adhesion molecules and formation of monocyte-thrombocyte aggregates in coronary restenosis].
OBJECTIVE
To examine expression of superficial antigens by blood monocytes and granulocytes as well as the number of leukocyte-platelet complexes forming in in vitro activation in patients subjected to coronary angioplasty; to analyse changes in these parameters in coronary restenosis.


METHODS
Membrane expression of leukocytic antigens and the number of leukocyte-platelet complexes after activation in the whole blood were measured by direct immunofluorescence and flow cytometry in 24 patients who have undergone stenting of coronary arteries. 14 of them had angiographically confirmed restenosis.


RESULTS
The tests discovered high expression of integrins Mac-1 and VLA-4 by monocytes and elevated relative number of monocyte-platelet complexes in patients with restenosis vs those free of stenosis (1425 +/- 76 and 1195 +/- 71 r.u. for Mac-1, 87 +/- 7 and 65 +/- 6 r.u. for VLA-4, 47 +/- 4 and 29 +/- 3%, respectively, for monocyte-platelet complexes; p < 0.05 for all the indices).


CONCLUSIONS
Coronary restenosis may result from elevated expression of adhesion molecules by monocytes manifest in activation of the cells in vitro.
Aref'eva
T I
TI
Provatorov
S I
SI
Samko
A N
AN
Baĭdun
L V
LV
Krasnikova
T L
TL
D015906:Angioplasty, Balloon, Coronary; D001792:Blood Platelets; D015815:Cell Adhesion Molecules / Q000097:blood*; D002449:Cell Aggregation; D023903:Coronary Restenosis / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D015854:Up-Regulation
D015815:Cell Adhesion Molecules