Direct electrochemistry of cytochrome c at a glassy carbon electrode modified with single-wall carbon nanotubes.
The electrochemistry of horse heart cytochrome c was studied by cyclic voltammetry at a glassy carbon electrode modified with single-wall carbon nanotubes (SWNTs). A pair of well-defined redox waves was obtained in cytochrome c aqueous solution at an activated SWNT film-modified electrode. The optimal conditions for activating the SWNT film-modified electrode has been determined. The electrode reaction of cytochrome c is a diffusion-controlled process. The peak current increases linearly with the concentration of cytochrome c in the range from 3.0 x 10(-5)-7.0 x 10(-4) M. The detection limit is 1.0 x 10(-5) M. The activated SWNT film was characterized by scanning electron microscopy. Furthermore, interaction of cytochrome c with adenine was characterized by electrochemical and spectral methods.
Wang
Jianxiu
J
College of Chemistry and Molecular Engineering, Peking University, Beijing, People's Republic of China.
Li
Meixian
M
Shi
Zujin
Z
Li
Nanqiang
N
Gu
Zhennan
Z
D000225:Adenine / Q000378:metabolism; D000818:Animals; D003574:Cytochrome c Group / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D006736:Horses; D006863:Hydrogen-Ion Concentration; D036103:Nanotechnology / Q000295:instrumentation; D010084:Oxidation-Reduction; D011485:Protein Binding; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D003574:Cytochrome c Group; D000225:Adenine