In vitro development of Haplorchis taichui (Trematoda: Heterophyidae).
Newly excysted metacercariae of Haplorchis taichui were cultured in a candle jar set at 37 degrees C. Both monophasic culture media [0.85% NaCl, RPMI 1640, RPMI 1640+10% fetal calf serum (FCS)] and diphasic culture media [RPMI 1640 + egg yolk agar, RPMI 1640 + 5%, 10% or 15% blood in blood agar (BA), RMPI 1640 + 5%, 10% and 15% FCS with 5% blood in BA] were used in vitro. Parasites survived for only 1 day in 0.85% NaCl without any development. In RPMI 1640 with egg yolk agar and RMPI 1640 + 5%, 10% FCS, the parasite survived for 3-5 days. In contrast, worms survived for 12-14 days in RPMI 1640 with blood agar without any change in result in a different concentration of blood in BA. The ovary and testes were observed after 3 days incubation in this media. Nevertheless, only 1 parasite in RPMI 1640 with 15% blood in BA had vitellaria and eggs at day 6. RPMI 1640 with blood agar can be used as short-term maintenance for the in vitro culture of H. taichui. However, further studies are needed.
Chaithong
U
U
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand.
Sukontason
K
K
Boonsriwong
N
N
Sukontason
K L
KL
Piangjai
S
S
D000818:Animals; D003470:Culture Media / Q000032:analysis / Q000737:chemistry*; D017077:Culture Media, Conditioned; D003530:Cyprinidae / Q000469:parasitology; D005393:Fish Diseases / Q000469:parasitology; D006578:Heterophyidae / Q000254:growth & development*; D066298:In Vitro Techniques; D013696:Temperature; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary
D003470:Culture Media; D017077:Culture Media, Conditioned