title: Abnormal accumulation in lipopolysaccharide in biliary epithelial cells of rats with self-filling blind loop.
abstract: Primary sclerosing cholangitis (PSC) is known to be frequently associated with inflammatory bowel diseases. In a rat with self-filling blind loop (SFBL), a proposed animal model for PSC, hepatobiliary inflammation has previously been demonstrated. In this study, we assessed the involvement of lipopolysaccharide (LPS), a bacterial endotoxin, in the pathogenesis of hepatobiliary inflammation of the SFBL model. The hepatic localization of LPS was examined by immunohistochemistry using an anti-lipid A antibody. The portal blood concentration of LPS was measured by an endotoxin-specific chromogenic Limulus test (Endospecy test). LPS was localized in the biliary epithelial cells (BECs) of rats with SFBL, and the portal blood concentration of LPS was significantly higher than that of sham-operated rats. Development of hepatobiliary inflammation, peribiliary fibrosis, and injury to the intestinal mucosa were histologically confirmed. Constriction in the biliary trees was radiologically demonstrated. These findings suggested that abnormal accumulation of LPS, which may be derived from portal blood, in BECs was involved in the pathogenesis of hepatobiliary inflammation with intestinal injury.
authors.affiliation: Second Department of Medicine, Kurume University School of Medicine, 830-0011, Japan. hirokoga@med.kurume-u.ac.jp
authors.forename: Hironori
authors.initials: H
authors.lastname: Koga
authors.forename: Shotaro
authors.initials: S
authors.lastname: Sakisaka
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshitake
authors.forename: Masaru
authors.initials: M
authors.lastname: Harada
authors.forename: Hiroto
authors.initials: H
authors.lastname: Kumemura
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Hanada
authors.forename: Eitaro
authors.initials: E
authors.lastname: Taniguchi
authors.forename: Takumi
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: Ryukichi
authors.initials: R
authors.lastname: Kumashiro
authors.forename: Michio
authors.initials: M
authors.lastname: Sata
mesh_terms: D000818:Animals; D001652:Bile Ducts / Q000033:anatomy & histology* / Q000473:pathology*; D002758:Cholangiography; D004847:Epithelial Cells / Q000378:metabolism*; D007150:Immunohistochemistry; D007249:Inflammation; D008050:Lipid A / Q000276:immunology; D008070:Lipopolysaccharides / Q000097:blood / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D008050:Lipid A; D008070:Lipopolysaccharides