title: T-cell factor-4 frameshift mutations occur frequently in human microsatellite instability-high colorectal carcinomas but do not contribute to carcinogenesis.
abstract: Colorectal carcinomas with microsatellite instability accumulate errors in short repetitive DNA repeats, especially mono and dinucleotide repeats. One such error-prone A(9) monorepeat is found in exon 17 of the TCF-4 gene. TCF-4 and beta-catenin form a transcription complex, which is important for both maintenance of normal epithelium and development of colorectal tumors. To elucidate the relevance of frameshift mutations in the TCF-4 in colorectal carcinogenesis, a variety of investigations in human tumors and cell lines was performed. It was found that mutations in the TCF-4 A(9) repeat do not contribute to tumorigenesis and seem to be passenger mutations.
authors.affiliation: Pathologisches Institut, der Friedrich-Alexander-Universität Erlangen-Nürnberg, Krankenhausstrasse 8-10, D-91054 Erlangen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Ruckert
authors.forename: Elke
authors.initials: E
authors.lastname: Hiendlmeyer
authors.forename: Wolfgang M
authors.initials: WM
authors.lastname: Brueckl
authors.forename: Ursula
authors.initials: U
authors.lastname: Oswald
authors.forename: Kurt
authors.initials: K
authors.lastname: Beyser
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Dietmaier
authors.forename: Angela
authors.initials: A
authors.lastname: Haynl
authors.forename: Claudia
authors.initials: C
authors.lastname: Koch
authors.forename: Josef
authors.initials: J
authors.lastname: Rüschoff
authors.forename: Thomas
authors.initials: T
authors.lastname: Brabletz
authors.forename: Thomas
authors.initials: T
authors.lastname: Kirchner
authors.forename: Andreas
authors.initials: A
authors.lastname: Jung
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000235:genetics; D005091:Exons; D016368:Frameshift Mutation; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016296:Mutagenesis; D051820:TCF Transcription Factors; D015534:Trans-Activators; D058118:Transcription Factor 7-Like 2 Protein; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation; D014162:Transfection; D051176:beta Catenin
chemical_list: C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D051820:TCF Transcription Factors; C544894:TCF7L2 protein, human; D015534:Trans-Activators; D058118:Transcription Factor 7-Like 2 Protein; D014157:Transcription Factors; D051176:beta Catenin