Induction of cell death by pro-oxidant action of Moxa smoke.
Moxa smoke induced internucleosomal DNA fragmentation in human promyelocytic leukemic HL-60 cells, but not in other cell lines. The cytotoxic activity of Moxa smoke was significantly reduced by a popular antioxidant, N-acetyl-L-cysteine (NAC). Moxa smoke showed oxidation potential (measured by NO monitor) and produced carbon radical (measured by ESR spectroscopy). The addition of NAC significantly reduced both the oxidation potential and carbon radical intensity of Moxa smoke. Activity staining of polyacryamide gel electrophoresis of MnSOD revealed the possible modification of the conformation and/or activity of this enzyme at an early stage of HL-60 cell death. These data suggest that Moxa smoke induces cytotoxicity by its pro-oxidant action.
Hitosugi
Naoko
N
Department of Anesthesia, Saitama Medical School, Sakado, Japan.
Ohno
Rie
R
Hatsukari
Ikusuke
I
Nakamura
Shinichi
S
Mizukami
Satoshi
S
Nagasaka
Hiroshi
H
Matsumoto
Isao
I
Satoh
Kazue
K
Negoro
Takaharu
T
Hashimoto
Ken
K
Sakagami
Hiroshi
H
D000111:Acetylcysteine / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000628:therapy; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005609:Free Radicals / Q000378:metabolism; D018922:HL-60 Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015674:Mammary Neoplasms, Animal / Q000378:metabolism / Q000628:therapy; D008928:Mitochondria / Q000187:drug effects; D009071:Moxibustion; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D012468:Salivary Gland Neoplasms / Q000378:metabolism / Q000628:therapy; D012906:Smoke; D013482:Superoxide Dismutase / Q000378:metabolism; D014407:Tumor Cells, Cultured
D005609:Free Radicals; D017382:Reactive Oxygen Species; D012906:Smoke; D013482:Superoxide Dismutase; D000111:Acetylcysteine