[Gene diagnosis of hemophilia B by multiple STR analysis].
OBJECTIVE
To establish a simple, rapid genetic diagnostic system for haemophilia B.


METHODS
The polymorphisms of eight STR loci in 87 normal persons and 8 haemophilia B families were assayed by PCR and genescan, and the linkage relations were analysed.


RESULTS
Six of the eight STR loci can provide genetic information for haemophilia B, and the heterozygosity is 0.50 approximately 0.83, PIC 0.39 approximately 0.80, and DP 0.66 approximately 0.94.


CONCLUSIONS
Combination of multiple STR loci analysis could be effective method for genetic diagnosis of haemophilia B.
Liu
Xiangfan
X
Shanghai Ruijin Hospital, Shanghai Institute of Hematology, Shanghai 200025, China.
Wang
Xuefeng
X
Fan
Qishi
Q
Chu
Haiyan
H
Fang
Yi
Y
Wang
Hongli
H
D005260:Female; D006580:Genetic Carrier Screening / Q000379:methods; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D002836:Hemophilia B / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010375:Pedigree; D011110:Polymorphism, Genetic