GATA proteins are potential negative regulators of HDC gene expression in the gastric epithelium.
In this study, we used the gastric epithelial cell line AGS-G(R) to investigate the role of GATA transcription factors in the regulation of both basal and gastrin-stimulated L-histidine decarboxylase (HDC) gene transcription. Using reporter gene technology, we compared the transcriptional activity of a construct, hHDC503, which contained the 5'-flanking region of the human HDC gene with that of similar constructs lacking selected GATA consensus sequences. We demonstrated the expression of GATA-4 and GATA-6 proteins within the AGS-G(R) cells and found evidence that these transcription factors can negatively regulate HDC gene expression.
Watson
Fiona
F
The Department of Pathology, Duncan Building, The University of Liverpool, Daulby Street, Liverpool L69 3GA, UK. watso@liv.ac.uk
Kiernan
Rachel S
RS
Dimaline
Rod
R
D002460:Cell Line; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D050991:GATA4 Transcription Factor; D050993:GATA6 Transcription Factor; D005755:Gastrins / Q000037:antagonists & inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D006640:Histidine Decarboxylase / Q000096:biosynthesis*; D006801:Humans; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D050991:GATA4 Transcription Factor; D050993:GATA6 Transcription Factor; C494759:GATA6 protein, human; D005755:Gastrins; D014157:Transcription Factors; D006640:Histidine Decarboxylase