High-performance liquid chromatographic method for the quantification of unbound evernimicin in human plasma ultrafiltrate.
A rapid HPLC method was developed for quantification of unbound evernimicin in human plasma. Protein-free samples prepared by ultrafiltration were injected directly onto a polymeric reversed-phase column and the eluent monitored at 302 nm. Evernimicin that eluted within 3.5 min was well resolved from endogenous components. Linearity was established between peak height and evernimicin concentration from 25 to 2500 ng/ml. Assay precision (C.V.) was within 5% while bias was no greater than 3%. This method has been used for the ex vivo assessment of evernimicin protein binding in human plasma from safety and tolerance as well as liver dysfunction and renal insufficiency studies.
Zhong
Ruyun
R
Department of Drug Metabolism and Pharmacokinetics, Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA. ruyun.zhong@spcorp.com
Hernandez
Abraham
A
Alton
Kevin B
KB
Kishnani
Narendra S
NS
Patrick
James E
JE
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014462:Ultrafiltration
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C001202:evernimicin