Natural variability in the Arabidopsis response to infection with Erwinia carotovora subsp. carotovora.
The natural variation in the response of Arabidopsis thaliana (L.) Heynh. to Erwinia carotovora subsp. carotovora has been studied in seven ecotypes and two mutants. The susceptibility of all the plant types was investigated by (i) macroscopic symptoms, (ii) fluorescence microscopy using green fluorescent protein (GFP) and (iii) bacterial growth in planta. Although all the plants were susceptible to the bacterium, there was no correlation in the degree of infection as ascertained by the three methods. The induction, upon infection, of several genes known to be involved in defense was analyzed by RNA blot hybridization. The patterns of expression of these genes differed according to the genotype. These results suggest that both salicylic and jasmonic acid play a role in the response of Arabidopsis to this bacterium.
Aguilar
Isabel
I
Departamento de Biotecnología - Universidad Politécnica de Madrid, E.T.S. Ingenieros Agrónomos, Ciudad Universitaria s/n, 28040 Madrid, Spain.
Alamillo
Josefa M
JM
García-Olmedo
Francisco
F
Rodríguez-Palenzuela
Pablo
P
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins; D007113:Immunity, Innate / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009154:Mutation; D016973:Pectobacterium carotovorum / Q000235:genetics / Q000254:growth & development*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D008164:Luminescent Proteins; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins