Quantitative determination for cytotoxic Friedo-nor-oleanane derivatives from five morphological types of Maytenus ilicifolia (Celastraceae) by reverse-phase high-performance liquid chromatography.
Five different morphological types of Maytenus ilicifolia of the same age and harvested under the same conditions showed distinct accumulations of some friedo-nor-oleananes. A rapid, sensitive and reliable reverse-phase HPLC method (employing an external standard) was used for the determination of the cytotoxic triterpenoids, 20 alpha-hydroxymaytenin, 22 beta-hydroxymaytenin, maytenin, celastrol and pristimerin in each of the five types. Well resolved peaks with good detection response and linearity in the range 1.0-100 micrograms/mL were obtained.
Buffa Filho
Waldemar
W
Núcleo de Bioensaio, Biossíntese e Ecofisiologia de Produtos Naturais, Instituto de Química, Universidade Estadual Paulista, CP. 355, 14801-970, Araraquara-SP, Brazil.
Corsino
Joaquim
J
Bolzani
da Silva Vanderlan
da SV
Furlan
Maysa
M
Pereira
Ana Maria S
AM
França
Suzelei Castro
SC
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification; D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029755:Maytenus / Q000033:anatomy & histology / Q000737:chemistry* / Q000145:classification; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives; D024301:Plant Bark / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012015:Reference Standards; D012680:Sensitivity and Specificity; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes; C413246:oleanane; D009828:Oleanolic Acid