title: Exercise and T-lymphocyte function: a comparison of proliferation in PBMC and NK cell-depleted PBMC culture.
abstract: This study utilized recently developed microbead technology to remove natural killer (NK) cells from peripheral blood mononuclear cell (PBMC) preparations to determine the effect of acute exercise on T-lymphocyte function, independent of changes in lymphocyte subpopulations. Twelve well-trained male runners completed a 60-min exercise trial at 95% ventilatory threshold and a no-exercise control trial. Six blood samples were taken at each session: before exercise, midexercise, immediately after exercise, and 30, 60, and 90 min after exercise. Isolated PBMC and NK cell-depleted PBMC were stimulated with the mitogen phytohemagglutinin. Cellular proliferation was assessed by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye uptake. In the PBMC cultures, there was a significantly lower mitogen response to phytohemagglutinin in exercise compared with the control condition immediately postexercise. There were no significant differences between the control and exercise conditions in NK cell-depleted PBMC cultures or in the responses adjusted for the percentage of CD3 cells. The present findings do not support the view that T-lymphocyte function is reduced after exercise.
authors.affiliation: School of Human Movement Studies, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia. kateG@qimr.edu.au
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Green
authors.forename: David G
authors.initials: DG
authors.lastname: Rowbottom
authors.forename: Laurel T
authors.initials: LT
authors.lastname: Mackinnon
mesh_terms: D000328:Adult; D017252:CD3 Complex / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007694:Killer Cells, Natural; D007937:Leukapheresis; D007958:Leukocyte Count; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012016:Reference Values; D012420:Running; D013601:T-Lymphocytes / Q000502:physiology*; D013997:Time Factors
chemical_list: D017252:CD3 Complex; D010835:Phytohemagglutinins