title: Tryptophan 80 and leucine 143 are critical for the hydride transfer step of thymidylate synthase by controlling active site access.
abstract: Mutant forms of thymidylate synthase (TS) with substitutions at the conserved active site residue, Trp 80, are deficient in the hydride transfer step of the TS reaction. These mutants produce a beta-mercaptoethanol (beta-ME) adduct of the 2'-deoxyuridine-5'-monophosphate (dUMP) exocyclic methylene intermediate. Trp 80 has been proposed to assist hydride transfer by stabilizing a 5,6,7,8-tetrahydrofolate (THF) radical cation intermediate [Barrett, J. E., Lucero, C. M., and Schultz, P. G. (1999) J. Am. Chem. Soc. 121, 7965-7966.] formed after THF changes its binding from the cofactor pocket to a putative alternate site. To understand the molecular basis of hydride transfer deficiency in a mutant in which Trp 80 was changed to Gly, we determined the X-ray structures of this mutant Escherichia coli TS complexed with dUMP and the folate analogue 10-propargyl-5,8-dideazafolate (CB3717) and of the wild-type enzyme complexed with dUMP and THF. The mutant enzyme has a cavity in the active site continuous with bulk solvent. This cavity, sealed from bulk solvent in wild-type TS by Leu 143, would allow nucleophilic attack of beta-ME on the dUMP C5 exocyclic methylene. The structure of the wild-type enzyme/dUMP/THF complex shows that THF is bound in the cofactor binding pocket and is well positioned to transfer hydride to the dUMP exocyclic methylene. Together, these results suggest that THF does not reorient during hydride transfer and indicate that the role of Trp 80 may be to orient Leu 143 to shield the active site from bulk solvent and to optimally position the cofactor for hydride transfer.
authors.affiliation: Macromolecular Structure Group, Department of Biochemistry and Biophysics, The University of California-San Francisco, San Francisco, California 94143-0448, USA.
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Fritz
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.forename: Janet S
authors.initials: JS
authors.lastname: Finer-Moore
authors.forename: Robert M
authors.initials: RM
authors.lastname: Stroud
mesh_terms: D001665:Binding Sites / Q000502:physiology; D001692:Biological Transport / Q000502:physiology; D003856:Deoxyuracil Nucleotides / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D005492:Folic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D007930:Leucine / Q000737:chemistry / Q000378:metabolism*; D008956:Models, Chemical; D017354:Point Mutation; D011522:Protons; D011799:Quinazolines / Q000378:metabolism; D013763:Tetrahydrofolates / Q000378:metabolism*; D013940:Thymidylate Synthase / Q000378:metabolism*; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003856:Deoxyuracil Nucleotides; D011522:Protons; D011799:Quinazolines; D013763:Tetrahydrofolates; C013123:5,10-methylenetetrahydrofolic acid; C031662:CB 3717; D014364:Tryptophan; D005492:Folic Acid; C007267:2'-deoxyuridylic acid; D013940:Thymidylate Synthase; D007930:Leucine