title: Increase in oxidative stress in kidneys of diabetic Akita mice.
abstract: New biomarkers for oxidative damage, were used to identify whether hyperglycemia caused oxidative stress in diabetic Akita mice. At 13 weeks of age, the tissues of these mice were obtained, and the levels of N(epsilon)-(hexanonyl)lysine (HEL) and dityrosine (DT) were measured, these being related to lipid peroxide-derived protein covalent modification and protein cross-linking. The levels of HEL and DT in the kidneys of Akita mice were significantly increased compared with the control mice without any accumulation of thiobarbituric acid reactive substances and 4-hydroxy-2-nonenal-modified protein. Immunopositive staining was clearly observed in the kidneys of the Akita mice when using the anti-HEL antibody or anti-DT antibody. These results suggest that hyperglycemia in Akita mice induced oxidative stress and increased these markers in the kidneys.
authors.affiliation: Nagoya University Graduate School of Bioagricultural Sciences, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ueno
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Horio
authors.forename: Koji
authors.initials: K
authors.lastname: Uchida
authors.forename: Michitaka
authors.initials: M
authors.lastname: Naito
authors.forename: Hideki
authors.initials: H
authors.lastname: Nomura
authors.forename: Yoji
authors.initials: Y
authors.lastname: Kato
authors.forename: Takanori
authors.initials: T
authors.lastname: Tsuda
authors.forename: Shinya
authors.initials: S
authors.lastname: Toyokuni
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Osawa
mesh_terms: D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D007668:Kidney / Q000473:pathology / Q000503:physiopathology*; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D014443:Tyrosine; D008239:Lysine