[Effect of dehydration and dDAVP on water permeability of basolateral membranes of epithelial cells in the kidney collecting tubules].
Water permeability of the outer medullary collecting duct's (OMCD) basolateral membrane was determined in vitro in the tubules isolated from hyperhydrated or dehydrated Wistar rats. Oil was injected into the lumen to block apical membrane water permeability. OMCD fragments underwent a hypoosmic shock (600/300 mOsm) and epithelial cells volume increased ad recorded with a digital camera. The latter's rate was used to calculate apparent water permeability of the membrane (Pf). Treatment of the tubules with Hg2Cl2 suppressed the water permeability. Water deprivation and dDAVP induced an increase in the basolateral water permeability. The data obtained suggest that the water permeability of the OMCD basolateral membrane may be stimulated by vasopressin and water deprivation.
Solenov
E I
EI
Institute of Cytology and Genetics, Russian Acad. Sci., Siberian Branch, 10 Pr. Lavrentyeva, Novosibirsk, 630090, Russia.
Baturina
G S
GS
Nesterov
V V
VV
Khodus
G R
GR
Ivanova
L N
LN
D000818:Animals; D001692:Biological Transport; D002463:Cell Membrane Permeability; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology*; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017483:Receptors, Vasopressin / Q000819:agonists; D019459:Urothelium / Q000378:metabolism; D014867:Water / Q000378:metabolism*; D014868:Water Deprivation
D017483:Receptors, Vasopressin; D014867:Water; D003894:Deamino Arginine Vasopressin