Single-chain antibodies produced by phage display against the C-terminal 19 kDa region of merozoite surface protein-1 of Plasmodium yoelii reduce parasite growth following challenge.
Antibodies have the potential to be therapeutic reagents for malaria. Here we describe the production of a novel phage antibody display library against the C-terminal 19kDa region of the Plasmodium yoelii YM merozoite surface protein-1 (MSP1(19)). In vivo studies against homologous lethal malaria challenge show an anti-parasite effect in a dose dependent manner, and analysis by plasmon resonance indicates binding to the antigen is comparable to the binding of a protective monoclonal antibody. The data support the lack of a need for any antibody Fc-related function and hold great significance for the development of a therapeutic reagent for malaria.
Vukovic
Peter
P
Cooperative Research Centre for Vaccine Technology, Queensland Institute of Medical Research, 300 Herston Road, P.O. Royal Brisbane Hospital, Herston, Qld. 40029, Australia.
Chen
Ke
K
Qin Liu
Xue
X
Foley
Michael
M
Boyd
Andrew
A
Kaslow
David
D
Good
Michael F
MF
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000627:therapeutic use*; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D007116:Immunization, Passive; D008288:Malaria / Q000188:drug therapy* / Q000276:immunology; D020066:Merozoite Surface Protein 1 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D019151:Peptide Library; D010967:Plasmodium yoelii / Q000187:drug effects / Q000254:growth & development / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D020066:Merozoite Surface Protein 1; D019151:Peptide Library