Pap1-mediated regulation of thioredoxin gene from Schizosaccharomyces pombe.
The genomic DNA encoding thioredoxin (TRX) was previously isolated from the fission yeast Schizosaccharomyces pombe. In this investigation, regulation of the S. pombe TRX gene was studied in lacZ translational fusions. The synthesis of beta-galactosidase from the fusion plasmid pYKT24 was significantly enhanced by treatments with cadmium chloride, zinc chloride, and high temperatures. Synthesis of beta-galactosidase from the fusion plasmid was significantly decreased by higher concentrations (5 microM, 10 microM) of mercuric chloride, whereas it was enhanced by its lower concentration (1 microM). Diamide affected the synthesis of beta-galactosidase in the same manner with mercuric chloride. However, high osmolarity had no effect on the beta-galactosidase synthesis from the fusion plasmid pYKT24. Various fusion plasmids were constructed to carry serially deleted upstream regions of the TRX gene. Pap1 mediates the regulation of the S. pombe TRX gene. The upstream region, between 987 and 1,270 bp from the translational initiation point, is responsible for the regulation.
Cho
Young-Wook
YW
Division of Life Sciences, Kangwon National University, Chuncheon, Korea.
Kim
Daemyung
D
Park
Eun-Hee
EH
Lim
Chang-Jin
CJ
D050976:Basic-Leucine Zipper Transcription Factors; D019256:Cadmium Chloride / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008627:Mercuric Chloride / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D000073718:Pancreatitis-Associated Proteins; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D013879:Thioredoxins / Q000235:genetics* / Q000378:metabolism; D024483:Vitamin K 3 / Q000494:pharmacology; D017967:Zinc Compounds / Q000494:pharmacology; D001616:beta-Galactosidase / Q000378:metabolism
D050976:Basic-Leucine Zipper Transcription Factors; D002712:Chlorides; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D016877:Oxidants; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; C000612141:REG3A protein, human; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D017967:Zinc Compounds; D013879:Thioredoxins; D008627:Mercuric Chloride; D024483:Vitamin K 3; C016837:zinc chloride; D006861:Hydrogen Peroxide; D001616:beta-Galactosidase; D019256:Cadmium Chloride