title: The structure and function of catalytic domains within inositol polyphosphate 5-phosphatases.
abstract: Phosphoinositide signaling pathways regulate many essential cellular functions including proliferation, differentiation and survival, cytoskeletal organization, and vesicular trafficking. The inositol polyphosphate 5-phosphatases regulate the cellular levels of several bioactive phosphoinositide species. This review describes the structure and function of the 5-phosphatase and Sac1 catalytic domains of these enzymes. The crystal structure of the 5-phosphatase domain has been solved and shares homology with members of the AP endonuclease family. The phosphoinositide polyphosphatase activity of the Sac1 domain, found in some inositol polyphosphate 5-phosphatases, is defined by a motif, CX5 R(T/S), also found in both protein and lipid phosphatases.
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Victoria, Australia. James.Whisstock@med.monash.edu.au
authors.forename: J C
authors.initials: JC
authors.lastname: Whisstock
authors.forename: F
authors.initials: F
authors.lastname: Wiradjaja
authors.forename: J E
authors.initials: JE
authors.lastname: Waters
authors.forename: R
authors.initials: R
authors.lastname: Gurung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019757:Carbon-Oxygen Lyases / Q000737:chemistry / Q000235:genetics; D020134:Catalytic Domain; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D019143:Evolution, Molecular; D006801:Humans; D000072181:Inositol Polyphosphate 5-Phosphatases; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D015398:Signal Transduction; D013379:Substrate Specificity
chemical_list: D009419:Nerve Tissue Proteins; C097702:synaptojanin; D010744:Phosphoric Monoester Hydrolases; D000072181:Inositol Polyphosphate 5-Phosphatases; D019757:Carbon-Oxygen Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase