Quantification and purity determination of newly synthesized thioacridines by capillary liquid chromatography.
Capillary liquid chromatography (CLC) was applied for quantification and impurity profile determination of ten newly synthesized acridine thioderivatives. A reversed-phase CLC system employing two different stationary phases, Nucleosil C18 and LiChrosorb RP-select B, was used. The mobile phase composition was optimized to get a satisfactory separation of impurities from the main acridine component in a reasonable analysis time. Significant differences in the chromatographic behavior between acridine derivatives containing and lacking amino groups were observed. Optimized separation conditions were used in CLC to measure the calibration curves of the acridine derivatives in a concentration range from 1.0 x 10(-6) to 1.0 x 10(-3) M at two different detector wavelengths (214 and 230 nm). Limits of detection and quantification of all the substances were determined. The detection limits went down to units of microM for most of the derivatives. CLC was also demonstrated to be a suitable method for the purity determination of test batches of the acridine thioderivatives.
Coufal
Pavel
P
Department of Analytical Chemistry, Faculty of Science, Charles University, Prague, Czech Republic. pcoufal@natur.cuni.cz
Bosáková
Zuzana
Z
Tesarová
Eva
E
Kafková
Bozena
B
Suchánková
Jana
J
Barbe
Jacques
J
D000166:Acridines / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000166:Acridines; D013438:Sulfhydryl Compounds