Quantification of sensitivity of endothelial cell markers for the astrocytoma and oligodendroglioma tumours.
Angiogenesis, the formation of new blood vessels, is necessary for tumour growth. The aim of this study was to quantify the sensitivity of five endothelial cell markers CD31/CD34-mix, CD31, CD34, lectin UEA-1 and FVIII-rag. Paraffin-embedded tumour tissue from 15 patients with astrocytomas grades II-IV and oligodendrogliomas grades II-III was used Sections of 2 microm were stained. Seven serial sections from each of the 15 tumours enabled consecutive examination of the vessels with all five markers and with two interposed hematoxylin-eosin (H&E) sections as controls. Among the endothelial cell markers, the mix CD31/CD34 showed the highest sensitivity with 79% and 99%, respectively, when applied in astrocytomas grade II and grades III-IV and 86% when applied in oligodendrogliomas. The study has shown that the sensitivity of the five endothelial cell markers varied. The mix CD31/CD34 was superior and seems to be a useful marker for quantification of angiogenesis.
Aronsson
Dhana E
DE
Department of Neuroscience, Neurology, Uppsala University, Sweden. dhana.aronsson@medcellbiol.uu.se
Muhr
Carin
C
D018952:Antigens, CD34 / Q000378:metabolism; D001254:Astrocytoma / Q000098:blood supply* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001932:Brain Neoplasms / Q000098:blood supply* / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism; D009837:Oligodendroglioma / Q000098:blood supply* / Q000473:pathology; D016612:Paraffin Embedding; D037121:Plant Lectins; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D012680:Sensitivity and Specificity; D014841:von Willebrand Factor / Q000378:metabolism
D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D037102:Lectins; D037121:Plant Lectins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C036850:Ulex europaeus lectins; D014841:von Willebrand Factor