[Induction of active forms of oxygen by biotic elicitors in tomato cell cultures].
The elicitor-induced generation of two oxygen species in tomato cell culture as well as their involvement into hypersensitive reaction was investigated. Generation of superoxide O2.- was measured by a lucigenin-related chemiluminescence. Accumulation of hydrogen peroxide H2O2 was measured by a fluorescent probe pyranin. Xylanase and chitosan were used as biotic elicitors with different mode of action. It was found that both O2.- and H2O2 had been accumulated in elicitor-treated tomato cells. The results obtained show that reactive oxidants are important signal transduction elements for activation of hypersensitive response in tomato cells.
Perkovskaia
G Iu
GIu
Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, Kyiv. gperk@uninet.kiev.ua
Kravchuk
Zh N
ZhN
Dmitriev
A P
AP
D002478:Cells, Cultured; D005456:Fluorescent Dyes; D008163:Luminescent Measurements; D018551:Solanum lycopersicum / Q000166:cytology / Q000378:metabolism*; D017382:Reactive Oxygen Species
D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species