Heterogeneity of dehydrogenases of Stenotrophomonas maltophilia showing dye-linked activity with polypropylene glycols.
Distinct enzyme activities were found in extracts from Stenotrophomonas maltophilia showing dye-linked oxidation of polypropylene glycols. The activities were induced when polypropylene glycols served as sole carbon and energy sources for the bacterium. In the logarithmic phase, most of the enzyme activities (88%) were found in the cytoplasm. In the stationary phase, more than half of the activities (54%) were found on the membrane, but significant activities were also distributed in the periplasm (34%) and the cytoplasm (12%). The enzyme activities differed from each other in their localization, time of induction in the growth cycle, specificity toward electron acceptor, and electrophoresis mobility.
Tachibana
Shinjiro
S
Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Okinawa, Japan.
Kawai
Fusako
F
Yasuda
Masaaki
M
D004396:Coloring Agents / Q000493:pharmacokinetics*; D003470:Culture Media; D007700:Kinetics; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D011108:Polymers / Q000493:pharmacokinetics*; D011409:Propylene Glycols / Q000493:pharmacokinetics*; D020615:Stenotrophomonas maltophilia / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity
D004396:Coloring Agents; D003470:Culture Media; D011108:Polymers; D011409:Propylene Glycols; C012504:polypropylene glycol; D010088:Oxidoreductases