Phospholipid and protein analysis of pulmonary surfactant.
Quantitative analysis of the phospholipid composition of pulmonary surfactant preparations has been achieved by a modification of the phosphorus-31 nuclear magnetic resonance method of E London and G Feigenson (J. Lipid Res. 20, 408-412, 1979). Resolution of the protein components by a 2-dimensional isoelectric-focussing-SDS/polyacrylamide-gel-electrophoresis technique is reported for the first time.
Cottrell
R C
RC
The British Industrial Biological Research Association, Carshalton, Surrey, UK.
Herod
I A
IA
Bayley
D
D
Purchase
R
R
Miller
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K
D000818:Animals; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000662:veterinary; D007525:Isoelectric Focusing / Q000379:methods / Q000662:veterinary; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010743:Phospholipids / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D011663:Pulmonary Surfactants / Q000737:chemistry* / Q000302:isolation & purification
D010743:Phospholipids; D011506:Proteins; D011663:Pulmonary Surfactants