title: Determination of Cr3+, CrO42-, and Cr2O72- in environmental matrixes by high-performance liquid chromatography with diode-array detection (HPLC-DAD).
abstract: A high-performance liquid chromatographic method with diode array detection (HPLC-DAD), based on chelation with ammonium pyrrolidinedithiocarbamate (APDC), has been developed for the determination of chromium species. Determination of Cr3+, CrO42-, and Cr2O72- was performed for standards and synthetic environmental matrixes. This method is robust, rugged, and can be used for rapid routine determination of chromium species with high precision and reliability. Sample pretreatment is simple. The method is capable of discriminating not only between Cr(III) and Cr(VI) but also between the chemical forms of Cr(VI) - CrO42- and Cr2O72-. By analysis of numerous samples the method has been shown to be selective, sensitive, and free from matrix interference, which is crucial for the determination of chromium species in difficult-to-analyze environmental matrixes. This method has been validated by means of an interlaboratory study. Although different speciation techniques were used during this study, there was good agreement between results from the two laboratories. The method detection limits were 7 and 4 mg L(-1) for Cr3+ and Cr2O72-, respectively. Recoveries of the analytes from spiked samples were 98% and 100% for Cr3+ and Cr2O72-, respectively. Both were based on a 10-mL sample volume spiked with 0.4 mg L(-1) chromium.
authors.affiliation: SAIC Canada, Ottawa, Ontario K1A 0H3, Canada. shamil.cathum@saic.com
authors.forename: S
authors.initials: S
authors.lastname: Cathum
authors.forename: C E
authors.initials: CE
authors.lastname: Brown
authors.forename: W
authors.initials: W
authors.lastname: Wong
mesh_terms: D002840:Chromates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002857:Chromium / Q000032:analysis*; D007202:Indicators and Reagents; D007753:Laboratories / Q000592:standards; D011786:Quality Control; D012680:Sensitivity and Specificity
chemical_list: D002840:Chromates; D007202:Indicators and Reagents; D002857:Chromium