Radicals, radical pairs and triplet states in photosynthesis.
The investigation of radical ions, radical pairs, and triplet states that occur in the primary processes of photosynthesis by various EPR techniques is described. The determination of the valence electron spin density distribution by ENDOR/TRIPLE spectroscopy is demonstrated for the primary electron donor. In combination with site-directed mutagenesis, these studies show that the spin distribution in the chlorophyll donor is strongly affected by the protein environment, and a link is established with the donor's oxidation potential and function in the electron transfer process. Similarities and differences between electron donors in anoxygenic (bacterial) and oxygenic photosynthesis are briefly discussed. Application of transient and pulse EPR techniques give information also on short-lived intermediates, such as radical pairs and triplet states, and allow the determination of distances and relative orientations of these species. The extension to time-resolved pulse ENDOR spectroscopy opens the possibility to resolve the electron-nuclear hyperfine structure of these reaction intermediates, even on a very short time scale.
Lubitz
Wolfgang
W
Max-Planck-Institut für Strahlenchemie, Stiftstrasse 34-36, 45470 Mülheim/Ruhr, Germany.
Lendzian
Friedhelm
F
Bittl
Robert
R
D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport / Q000235:genetics; D004583:Electrons; D005609:Free Radicals / Q000737:chemistry*; D045342:Light-Harvesting Protein Complexes; D010788:Photosynthesis / Q000235:genetics; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000235:genetics
D005609:Free Radicals; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins