Identification of a new HLA-B*15 allele, HLA-B*1569.
We have identified a new HLA-B*15 allele (B*1569) by polymerase chain reaction (PCR) using sequence-specific primers (SSP) and sequence-based typing (SBT). This novel allele was found in a 67-year-old white Caucasian male and differs from HLA-B*1503 at 3 positions. The nucleotide substitutions at positions 544, 559 and 560 result in amino acid changes at codon 158 from GCC (alanine) to ACC (threonine), and at codon 163 from CTG (leucine) to ACG (threonine).
Smillie
David M
DM
Histocompatibility Laboratory, National Blood Service, Sheffield, United Kingdom. david.smillie@nbs.nhs.uk
Smith
P A
PA
Day
S
S
Dunn
P P J
PP
D000368:Aged; D000483:Alleles; D001483:Base Sequence; D005091:Exons; D015235:HLA-B Antigens / Q000235:genetics*; D059910:HLA-B15 Antigen; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D044465:White People / Q000235:genetics
D015235:HLA-B Antigens; D059910:HLA-B15 Antigen