title: Production of sepiapterin in Escherichia coli by coexpression of cyanobacterial GTP cyclohydrolase I and human 6-pyruvoyltetrahydropterin synthase.
abstract: Synechocystis sp. strain PCC 6803 GTP cyclohydrolase I and human 6-pyruvoyltetrahydropterin synthase were coexpressed in Escherichia coli. The E. coli transformant produced sepiapterin, which was identified by high-performance liquid chromatography and enzymatically converted to dihydrobiopterin by sepiapterin reductase. Aldose reductase, another indispensable enzyme for sepiapterin production, may be endogenous in E. coli.
authors.affiliation: Department of Microbiology, Inje University, Kimhae 621-749, Korea.
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Woo
authors.forename: Jee Yun
authors.initials: JY
authors.lastname: Kang
authors.forename: Yong Kee
authors.initials: YK
authors.lastname: Choi
authors.forename: Young Shik
authors.initials: YS
authors.lastname: Park
mesh_terms: D000449:Aldehyde Reductase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000201:enzymology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006136:GTP Cyclohydrolase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019761:Phosphorus-Oxygen Lyases / Q000378:metabolism*; D011621:Pteridines / Q000378:metabolism*; D011622:Pterins; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D011621:Pteridines; D011622:Pterins; D011994:Recombinant Proteins; C016727:sepiapterin; D000449:Aldehyde Reductase; D006136:GTP Cyclohydrolase; D019761:Phosphorus-Oxygen Lyases; C043497:6-pyruvoyltetrahydropterin synthase