title: Effect of diethylsulphoxide on growth, survival and ion exchange of Escherichia coli.
abstract: OBJECTIVE
  To study the effect of diethylsulphoxide (DESO) on Escherichia coli growth, survival and ionic exchange in comparison with dimethylsulphoxide (DMSO).
  
  
  RESULTS
  Bacterial survival was estimated by counting colony-forming units and by the most probable number (five-tube) technique; the K+ and H+ transport and H(2) formation were determined electrochemically. Diethylsulphoxide at concentrations between 0.01 and 0.5% (w/v) stimulated and above 5% decreased the anaerobic growth rate and survival. 2H+ : K+ exchange and H(2) formation were lost at 5% DESO. At 0.05% DESO the kinetic characteristics of H+ : K+ exchange and H(2) formation were typical for Delta micro (H(+)) -dependent TrkA uncoupled with F(0)F(1) under respiration.
  
  
  CONCLUSIONS
  Diethylsulphoxide at low concentrations serves as an electron acceptor for an anaerobic respiratory chain stimulating bacterial growth and survival through the modulation of H+ : K+ exchange and H(2) formation activity. The effects of DESO were more pronounced than those of DMSO.
  
  
  CONCLUSIONS
  Diethylsulphoxide determines essential biological and therapeutic properties that make its application preferable.
authors.affiliation: Department of Chemistry, Yerevan State University, Yerevan, Armenia.
authors.forename: S A
authors.initials: SA
authors.lastname: Markarian
authors.forename: A A
authors.initials: AA
authors.lastname: Poladyan
authors.forename: G R
authors.initials: GR
authors.lastname: Kirakosyan
authors.forename: A A
authors.initials: AA
authors.lastname: Trchounian
authors.forename: K A
authors.initials: KA
authors.lastname: Bagramyan
mesh_terms: D000473:Alkanes / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D007474:Ion Exchange; D011188:Potassium / Q000378:metabolism; D013454:Sulfoxides / Q000494:pharmacology*
chemical_list: D000473:Alkanes; D013454:Sulfoxides; D006859:Hydrogen; D011188:Potassium; D004121:Dimethyl Sulfoxide