Normal gene expression in male F344 rat nasal transitional and respiratory epithelium.
The nasal epithelium is an important target site for chemically-induced toxicity and carcinogenicity in rodents. Gene expression profiles were determined in order to provide normal baseline data for nasal transitional/respiratory epithelium from healthy rats. Cells lining the rat nasal passages were collected and gene expression analysis was performed using Clontech cDNA Rat Atlas 1.2 arrays (1185 genes). The percentages of genes within specific average expression ranges were 4.2% at 45,000-1000, 14.8% at 1000-200, 25.0% at 200-68, and 56.0% below 68. Nine out of a subset of ten genes were confirmed for relative signal intensity using quantitative real-time RT-PCR. The most highly expressed genes included those involved in phase I (e.g. cytochrome P450s) and phase II (e.g. glutathione S-transferases) xenobiotic metabolism, bioenergetics (e.g. cytochrome oxidase), osmotic balance (e.g. Na(+)/K(+) ATPase) and epithelial ionic homeostasis (e.g. ion channels). Such baseline data will contribute to further understanding the normal physiology of these cells and facilitate the interpretation of responses by the nasal epithelial cells to xenobiotic treatment or disease.
Hester
Susan D
SD
US EPA, Research Triangle Park, NC, USA. hester.susan@epamail.epa.gov
Benavides
Gina B
GB
Sartor
Maureen
M
Yoon
Lawrence
L
Wolf
Douglas C
DC
Morgan
Kevin T
KT
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D004848:Epithelium / Q000378:metabolism; D020869:Gene Expression Profiling; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344 / Q000235:genetics*; D020545:Respiratory Mucosa / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D012313:RNA