Cytokines affect procathepsin D-stimulated proliferation of breast cancer cells.
Procathepsin D is over secreted by certain human cancer cells. This enzymatically inactive precursor has been established as playing an important role in the development of several types of cancer. Due to their pleiotropical effects, numerous cytokines have also been recognized as important immunotherapeutic agents. In this study, we focused on the role of IL-4, IL-10 and IL-13 on procathepsin D-stimulated proliferation of breast cancer cells. Our results clearly showed that only ER+ breast cancer cells responded to the presence of cytokines by proliferation; ER- cells were resistant to the addition of cytokines. Since addition of anti-procathepsin D antibodies blocked the growth potentiation, we concluded that addition of these cytokines resulted in stimulation of synthesis and/or release of procathepsin D. This conclusion was further supported by findings of procathepsin D in culture supematants of cells incubated with cytokines.
Voburka
Zdenek
Z
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague.
Vetvicka
Vaclav
V
Vetvickova
Jana
J
Fusek
Martin
M
D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002402:Cathepsin D / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D004792:Enzyme Precursors / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology; D018793:Interleukin-13 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000502:physiology; D014407:Tumor Cells, Cultured
D004792:Enzyme Precursors; D018793:Interleukin-13; D007378:Interleukins; D011960:Receptors, Estrogen; D016753:Interleukin-10; D015847:Interleukin-4; C058345:procathepsin D; D002402:Cathepsin D