A new improved method for extraction of DNA from teeth for the analysis of hypervariable loci.
A new method for better recovery of DNA suitable for amplification of hypervariable loci from fragments of teeth, consisting of two steps-scraping and aspiration, and extensive decalcification-is reported. Higher yields of high molecular weight DNA were obtained from the root, pulp, and crown of all kinds of 120 teeth, irrespective of gender, age, and source of teeth. HLA DQA1, 5 poly markers (LDLR, GYPA, HBGG, D7S8, and Gc), and other 12 short tandem repeat loci (HPRTB, F13B, LPL, D13S317, D7S820, D5S818, D21S11, D18S51, FGA, D8S1179, D3S1358, and vWA) could be successfully amplified and typed from recovered DNA.
Trivedi
R
R
DNA Typing Unit, Central Forensic Science Laboratory, Calcutta, India.
Chattopadhyay
P
P
Kashyap
V K
VK
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D004247:DNA / Q000032:analysis*; D005553:Forensic Dentistry / Q000379:methods*; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D013048:Specimen Handling; D020080:Tandem Repeat Sequences / Q000235:genetics*; D014070:Tooth / Q000737:chemistry*
D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D004247:DNA