Effect of acidic phospholipids on the structural properties of recombinant cytosolic human glyoxalase II.
A peculiar characteristic of highly concentrated cytosolic recombinant human glyoxalase II (GII) solutions is to undergo partial precipitation. Previous work indicated that anionic phospholipids (PLs) exert a noncompetitive inhibition on the enzymatic activity of the soluble enzyme. In this study, FTIR spectroscopy was used to analyze the structural properties and the thermal stability of the soluble protein in the absence and in the presence of liposomes made of different phospholipids (PLs). The structural analysis was performed on the precipitate as well. The interaction of acidic PLs with GII lowered the thermal stability of the enzyme and inhibited protein intermolecular interactions (aggregation) brought about by thermal denaturation. Infrared data indicated that ionic and hydrophobic interactions occur between GII and acidic PLs causing small changes in the secondary structure of the enzyme. No interactions of the protein with egg phosphatidylcholine liposomes were detected. The results are consistent with the destabilization of the protein tertiary structure, and indicate that GII possesses hydrophobic part(s) that interact with the acyl chains of PLs. Data on precipitated GII did not show remarkable modification of secondary structure, suggesting that hydrophobic stretches of the enzyme may also be involved in the protein-protein association (precipitation) at high GII concentration. The alterations in the GII structure and the noncompetitive inhibition exerted by acidic PLs are strictly related.
Scirè
Andrea
A
Institute of Biochemistry, University of Ancona, Via Ranieri, Ancona, Italy.
Saccucci
Franca
F
Bertoli
Enrico
E
Cambria
Maria Teresa
MT
Principato
Giovanni
G
D'Auria
Sabato
S
Tanfani
Fabio
F
D011232:Chemical Precipitation; D003600:Cytosol / Q000201:enzymology; D004795:Enzyme Stability; D004952:Esters / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes / Q000737:chemistry; D010713:Phosphatidylcholines / Q000494:pharmacology; D010743:Phospholipids / Q000737:chemistry / Q000494:pharmacology*; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013869:Thiolester Hydrolases / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000099301:Hydroxyacylglutathione Hydrolase
D004952:Esters; D005227:Fatty Acids; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D011994:Recombinant Proteins; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase