title: Transformation system of Beauveria bassiana and Metarhizium anisopliae using nitrate reductase gene of Aspergillus nidulans.
abstract: An heterologous transformation system for entomopathogenic fungi B. bassiana and M. anisopliae was developed based on the use of A. nidulans nitrate reductase gene (niaD). B. bassiana and M. anisopliae niaD stable mutants were selected by treatment of protoplast with ethane methane sulphonate (EMS) and regenerated on chlorate medium. The cloned gene was capable of transforming B. bassiana and M. anisopliae at a frequency of 5.8 to 20 transformants per microg of DNA. Most of them were mitotically stable.
authors.affiliation: Department of Biological Science, Rani Durgawati University, Jabalpur, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Sandhu
authors.forename: J R
authors.initials: JR
authors.lastname: Kinghorn
authors.forename: R C
authors.initials: RC
authors.lastname: Rajak
authors.forename: S E
authors.initials: SE
authors.lastname: Unkles
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics; D005800:Genes, Fungal; D006999:Hypocreales / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*; D008938:Mitosis; D009154:Mutation; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D009565:Nitrate Reductases; D050901:Nitrate Reductase