Integration host factor interactions with Neisseria gene sequences: correlation between predicted binding sites and in vitro binding of Neisseria -derived IHF protein.
Putative integration host factor (IHF) binding sites are frequently being identified in Neisseria gene sequences on the basis of similarity to a degenerate Escherichia coli -derived consensus binding sequence. In this report, three different Neisseria genetic systems that contain predicted IHF binding sites were assessed for IHF binding through gel retardation analysis. The results show a positive correlation between the identification of a predicted Neisseria IHF binding site and in vitro binding of Neisseria -derived IHF protein.
Hill
S A
SA
Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, US.
Samuels
D S
DS
Nielsen
C
C
Knight
S W
SW
Pagotto
F
F
Dillon
J A R
JA
D001483:Base Sequence; D001665:Binding Sites; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D034982:Integration Host Factors / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009343:Neisseria / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D016415:Sequence Alignment; D013223:Statistics as Topic
D004269:DNA, Bacterial; D034982:Integration Host Factors