Adenylate cyclase of myometrium and its regulation by cAMP-dependent protein kinase.
The purpose of this study was to examine activity of adenylate cyclase (AC) and its cAMP-dependent phosphorylation by cAMP-dependent protein kinase (PKA) in the membrane of rabbit myometrium. Isoproterenol (IP) significantly increased AC activity of nonpregnant rabbits myometrium plasma membranes. However, during pregnancy AC of myometrium plasma membranes did not respond to IP. Phosphorylation of the myometrium membrane by PKA was followed by significantly decreased response of AC to IP.
Shlykov
S G
SG
Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv. kinet@biochem.kiev.ua
Kalinski
M I
MI
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D005260:Female; D007545:Isoproterenol / Q000494:pharmacology; D009215:Myometrium / Q000187:drug effects / Q000201:enzymology*; D010766:Phosphorylation; D011247:Pregnancy; D011817:Rabbits
D017868:Cyclic AMP-Dependent Protein Kinases; D000262:Adenylyl Cyclases; D007545:Isoproterenol