Extracellular superoxide dismutase and glomerular mesangial cells: its production and regulation.
Extracellular superoxide dismutase (EC-SOD) is synthesized in mesenchymally derived cells and prevents the oxygen radical-induced injury. We studied whether kidney mesangial cells (MCs) produce EC-SOD and how its production is associated with chemokine secretion. Under unstimulated condition, MCs produced EC-SOD, and its production was correlated positively with cyclic adenosine monophosphate (cAMP), but negatively with interleukin (IL)-6 or IL-8 production. By prednisolone or phorbol myristate acetate treatment, EC-SOD levels were correlated negatively with levels of IL-6 and IL-8. The presence of adenylate cyclase inhibitor 2',3'-dideoxyadenosine lost the prednisolone effect. The stimulation of EC-SOD production might be one of the important effects of prednisolone via cAMP pathway in MCs.
Yamada
Harutaka
H
First Department of Internal Medicine, Aichi Medical University, Yazako-Karimata, Nagakute, Aichi, Japan. harutaka@amugw.aichi-med-u.ac.jp
Adachi
Tetsuo
T
Fukatsu
Atsushi
A
Misao
Sachiko
S
Yamada
Yasukazu
Y
Aoki
Takanari
T
Miura
Naoto
N
Sakuma
Masato
M
Nishikawa
Kazuhiro
K
Futenma
Arao
A
Kakumu
Shinichi
S
D000067956:Adenylyl Cyclase Inhibitors; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D016048:Dideoxyadenosine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D020536:Enzyme Activators / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism*; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D011239:Prednisolone / Q000037:antagonists & inhibitors / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000067956:Adenylyl Cyclase Inhibitors; D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D015850:Interleukin-6; D016209:Interleukin-8; D016048:Dideoxyadenosine; D011239:Prednisolone; D000242:Cyclic AMP; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate