title: Quantitative detection of respiratory Chlamydia pneumoniae infection by real-time PCR.
abstract: Real-time PCR was evaluated as a quantitative diagnostic method for Chlamydia pneumoniae infection using different respiratory samples. Real-time PCR had efficiency equal to or better than that of nested touchdown PCR. This study confirmed sputum as the best sampling material to detect an ongoing C. pneumoniae infection.
authors.affiliation: Department of Virology, University of Umeå, Umeå, Sweden. Yvonne.Kuoppa@climi.umu.se
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Kuoppa
authors.forename: Jens
authors.initials: J
authors.lastname: Boman
authors.forename: Lena
authors.initials: L
authors.lastname: Scott
authors.forename: Urban
authors.initials: U
authors.lastname: Kumlin
authors.forename: Iréne
authors.initials: I
authors.lastname: Eriksson
authors.forename: Annika
authors.initials: A
authors.lastname: Allard
mesh_terms: D023521:Chlamydophila Infections / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology*; D010614:Pharynx / Q000382:microbiology*; D018410:Pneumonia, Bacterial / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000382:microbiology*; D013183:Sputum / Q000382:microbiology*
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial