title: Molecular cloning, functional expression and characterization of d-limonene synthase from Agastache rugosa.
abstract: We cloned the gene of d-limonene synthase (ArLMS) from Agastache rugosa (Labiatae). The function of ArLMS was elucidated by the preparation of recombinant protein and subsequent enzyme assay. ArLMS consisted of 2077 nucleotides including 1839 bp of coding sequence that encodes a protein of 613 amino acids. This protein has a 60 kDa molecular weight, which is identical to that of d-limonene synthase from Schizonepeta tenuifolia (Labiatae). The deduced amino acid sequence of ArLMS shows high homology with the known d- and l-limonene synthases from Labiatae plants. Here, we discussed the amino acid residues responsible for the stereochemical regulation in limonene biosynthesis.
authors.affiliation: Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Maruyama
authors.forename: Daisuke
authors.initials: D
authors.lastname: Saeki
authors.forename: Michiho
authors.initials: M
authors.lastname: Ito
authors.forename: Gisho
authors.initials: G
authors.lastname: Honda
mesh_terms: D031327:Agastache / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D000081005:Cyclohexane Monoterpenes; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D019753:Intramolecular Lyases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D039821:Monoterpenes / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry; D011487:Protein Conformation
chemical_list: D000081005:Cyclohexane Monoterpenes; D018076:DNA, Complementary; D039821:Monoterpenes; D009822:Oils, Volatile; C039648:pulegone; D019753:Intramolecular Lyases; C040265:pinene cyclase I