Nucleotide sequence of mitochondrial CO I and ribosomal ITS II genes of Opisthorchis viverrini in northeast Thailand.
The mitochondrial cytochrome c oxidase subunit I (CO I) gene and the second internal transcribed spacer region (ITS II) gene of Opisthorchis viverrini were compared among O. viverrini from various areas in northeast Thailand. The nucleotide sequences of partial CO I gene (417 bp) of O. viverrini differed among O. viverrini originated from Ubon Ratana, Leongpleuy, Ban Phai, Maha Sarakham, and Chatturat. These intraspecific variations were classified into 5 patterns but no area-specific pattem was observed. Amino acid sequence deduced from the nucleotide sequences of these genes was identical. Nucleotide sequences of a region of the O. viverrini ITS II gene (296 bp) from different areas were identical. However, they were different from those of Clonorchis sinensis, Haplorchis taichui, H. pumilio, Fasciola gigantica, Echinostoma malayanum and Centrocestus sp..
Ando
K
K
Department of Medical Zoology, School of Medicine, Mie University, Tsu, Japan.
Sithithaworn
P
P
Nuchjungreed
C
C
Tesana
S
S
Srisawangwong
T
T
Limviroj
W
W
Chinzei
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D018509:DNA, Helminth / Q000737:chemistry*; D004272:DNA, Mitochondrial / Q000737:chemistry*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009889:Opisthorchiasis / Q000469:parasitology; D009891:Opisthorchis / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013785:Thailand
D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV