Development and validation of a liquid chromatographic method for Casiopeina IIIi in rat plasma.
A sensitive and specific liquid chromatographic method using extraction with zinc sulfate has been developed for the determination of Casiopeina IIIi and validated over the linear range 5-100 microg/ml in 1 ml of rat plasma. The analysis was performed on a Symmetry C(18) (5 microm) column. The mobile phase was methanol: 0.01 M phosphate buffer pH 6.5 (40:60, v/v). The column effluent was monitored at 262 nm. The results showed that the assay is sensitive at 5 microg/ml. Maximum intra-day coefficient of variation was 10.6%. The recovery obtained in plasma was 87.2%. The method was used to perform protein binding studies by equilibrium dialysis in rat plasma and was found to be satisfactory.
Fuentes-Noriega
Inés
I
Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, 04510 México D.F., Mexico.
Ruiz-Ramírez
Lena
L
Tovar Tovar
Araceli
A
Rico-Morales
Héctor
H
Gracia-Mora
Isabel
I
D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D009942:Organometallic Compounds / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000970:Antineoplastic Agents; D009942:Organometallic Compounds; C461490:casiopeina IIIi