title: Persistent rat cytomegalovirus (RCMV) infection of the salivary glands contributes to the anti-RCMV humoral immune response.
abstract: The salivary glands are the major sites of persistent replication of rat cytomegalovirus (RCMV). At several months post infection (pi), infectious RCMV is usually still produced in the salivary glands but not in any other organ or tissue of the rat. To investigate whether the persistence of RCMV in the salivary glands is crucial to the pathogenesis of viral infection, we monitored the progression of RCMV-induced disease in rats from which the salivary glands had been surgically removed (desalivated) as well as in sham-operated rats, both after a lethal and sublethal challenge with RCMV. Desalivation did not have a significant effect on either RCMV-induced morbidity or mortality. As expected, at 1 year pi, relatively high levels of infectious virus were detected in the salivary glands of sham-operated rats, whereas neither infectious virus nor RCMV DNA could be detected in liver, spleen and lungs of these animals. Infectious virus and viral DNA were also undetectable in organs from desalivated animals at 1 year pi. Surprisingly, a difference was found between desalivated and sham-operated rats in the titers of anti-RCMV IgG antibodies, which were significantly higher in sham-operated rats than in desalivated animals at 183, 295 and 365 days pi. This finding indicates that the persistence of RCMV in the salivary glands may contribute significantly to the anti-RCMV humoral immunity of infected rats.
authors.affiliation: Department of Medical Microbiology, Cardiovascular Research Institute Maastricht, University of Maastricht, PO Box 5800, 6202 AZ Maastricht, The Netherlands.
authors.forename: Jeroen S
authors.initials: JS
authors.lastname: Kloover
authors.forename: Anthony E
authors.initials: AE
authors.lastname: van den Bogaard
authors.forename: Joanne G
authors.initials: JG
authors.lastname: van Dam
authors.forename: Gert E L M
authors.initials: GE
authors.lastname: Grauls
authors.forename: Cornelis
authors.initials: C
authors.lastname: Vink
authors.forename: Cathrien A
authors.initials: CA
authors.lastname: Bruggeman
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D004195:Disease Models, Animal; D006566:Herpesviridae Infections / Q000097:blood / Q000276:immunology* / Q000821:virology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008297:Male; D018146:Muromegalovirus / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D012469:Salivary Glands / Q000276:immunology* / Q000601:surgery; D017735:Virus Latency / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G