Coordinated transcription of key pathways in the mouse by the circadian clock.
In mammals, circadian control of physiology and behavior is driven by a master pacemaker located in the suprachiasmatic nuclei (SCN) of the hypothalamus. We have used gene expression profiling to identify cycling transcripts in the SCN and in the liver. Our analysis revealed approximately 650 cycling transcripts and showed that the majority of these were specific to either the SCN or the liver. Genetic and genomic analysis suggests that a relatively small number of output genes are directly regulated by core oscillator components. Major processes regulated by the SCN and liver were found to be under circadian regulation. Importantly, rate-limiting steps in these various pathways were key sites of circadian control, highlighting the fundamental role that circadian clocks play in cellular and organismal physiology.
Panda
Satchidananda
S
The Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA.
Antoch
Marina P
MP
Miller
Brooke H
BH
Su
Andrew I
AI
Schook
Andrew B
AB
Straume
Marty
M
Schultz
Peter G
PG
Kay
Steve A
SA
Takahashi
Joseph S
JS
Hogenesch
John B
JB
D000818:Animals; D001483:Base Sequence; D001683:Biological Clocks / Q000235:genetics / Q000502:physiology*; D056926:CLOCK Proteins; D002453:Cell Cycle / Q000502:physiology; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D017403:In Situ Hybridization; D008099:Liver / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009154:Mutation; D013493:Suprachiasmatic Nucleus / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*
D015534:Trans-Activators; D014157:Transcription Factors; D056926:CLOCK Proteins; C538944:Clock protein, mouse