Regulatory and essential light chains of myosin rotate equally during contraction of skeletal muscle.
Myosin head consists of a globular catalytic domain and a long alpha-helical regulatory domain. The catalytic domain is responsible for binding to actin and for setting the stage for the main force-generating event, which is a "swing" of the regulatory domain. The proximal end of the regulatory domain contains the essential light chain 1 (LC1). This light chain can interact through the N and C termini with actin and myosin heavy chain. The interactions may inhibit the motion of the proximal end. In consequence the motion of the distal end (containing regulatory light chain, RLC) may be different from the motion of the proximal end. To test this possibility, the angular motion of LC1 and RLC was measured simultaneously during muscle contraction. Engineered LC1 and RLC were labeled with red and green fluorescent probes, respectively, and exchanged with native light chains of striated muscle. The confocal microscope was modified to measure the anisotropy from 0.3 microm(3) volume containing approximately 600 fluorescent cross-bridges. Static measurements revealed that the magnitude of the angular change associated with transition from rigor to relaxation was less than 5 degrees for both light chains. Cross-bridges were activated by a precise delivery of ATP from a caged precursor. The time course of the angular change consisted of a fast phase followed by a slow phase and was the same for both light chains. These results suggest that the interactions of LC1 do not inhibit the angular motion of the proximal end of the regulatory domain and that the whole domain rotates as a rigid body.
Borejdo
Julian
J
Department of Molecular Biology and Immunology, University of North Texas, Fort Worth, Texas 76107-2699, USA. jborejdo@hsc.unt.edu
Ushakov
Dmitry S
DS
Akopova
Irina
I
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D016880:Anisotropy; D055592:Biophysical Phenomena; D001703:Biophysics; D006801:Humans; D066298:In Vitro Techniques; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000502:physiology; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000502:physiology*; D018994:Myosin Light Chains / Q000737:chemistry* / Q000502:physiology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012399:Rotation
D018994:Myosin Light Chains; D011994:Recombinant Proteins; C491915:hALC-1 protein, human; C029273:P(3)-1-(2-nitro)phenylethyladenosine 5'-triphosphate; D000255:Adenosine Triphosphate