Enzymes of malate metabolism in Mesorhizobium ciceri CC 1192.
Electrophoretic studies were performed on enzymes concerned with the oxidation of malate in free-living and bacteroid cells of Mesorhizobium ciceri CC 1192, which forms nitrogen-fixing symbioses with chickpea (Cicer arietinum L.) plants. Two malate dehydrogenases were detected in extracts from both types of cells in native polyacrylamide electrophoresis gels that were stained for enzyme activity. One band of malate dehydrogenase activity was stained only in the presence of NADP+, whereas the other band was revealed with NAD+ but not NADP+. Further evidence for the occurrence of separate NAD- and NADP-dependent malate dehydrogenases was obtained from preliminary enzyme kinetic studies with crude extracts from free-living M. ciceri CC 1192 cells. Activity staining of electrophoretic gels also indicated the presence of two malic enzymes in free-living and bacteroid cells of M. ciceri CC 1192. One malic enzyme was active with both NAD+ and NADP+, whereas the other was specific for NADP+. Possible roles of the multiple forms of malate dehydrogenase and malic enzyme in nitrogen-fixing symbioses are discussed.
Tabrett
Catherine Ann
CA
Department of Agricultural Chemistry and Soil Science, University of Sydney, New South Wales, Australia.
Copeland
Les
L
D020561:Alphaproteobacteria / Q000201:enzymology* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D015964:Gene Expression Regulation, Bacterial; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D050538:Malate Dehydrogenase (NADP+); D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009586:Nitrogen Fixation; D018517:Plant Roots / Q000382:microbiology*; D013559:Symbiosis
D007527:Isoenzymes; D009243:NAD; D009249:NADP; D008291:Malate Dehydrogenase; D050538:Malate Dehydrogenase (NADP+)