Cloning of disease-resistance homologues in end sequences of BAC clones linked to Fom-2, a gene conferring resistance to Fusarium wilt in melon (Cucumis melo L.).
Disease resistance has not yet been characterized at the molecular level in cucurbits, a group of high-value, nutritious, horticultural plants. Previously, we genetically mapped the Fom-2 gene that confers resistance to Fusarium wilt races 0 and I of melon. In this paper, two cosegregating codominant markers (AM, AFLP marker; FM, Fusarium marker) were used to screen a melon bacterial artificial chromosome (BAC) library. Identified clones were fingerprinted and end sequenced. Fingerprinting analysis showed that clones identified by each marker assembled into two separate contigs at high stringency. GenBank searches produced matches to leucine-rich repeats (LRRs) of resistance genes (R genes); to retroelements and to cellulose synthase in clones identified by FM; and to nucleotide-binding sites (NBSs) of R genes, retroelements, and cytochrome P-450 in clones identified by AM. A 6.5-kb fragment containing both NBS and LRR sequences was found to share high homology to TIR (Toll-interleukin-1 receptor)-NBS-LRR R genes, such as N, with 42% identity and 58% similarity in the TIR-NBS and LRR regions. The sequence information may be useful for identifying NBS-LRR class of R genes in other cucurbits.
Wang
Yi-Hong
YH
Clemson University Genomics Institute and Department of Plant Pathology and Physiology, Clemson University, SC 29634, USA.
Choi
Woobong
W
Thomas
Claude E
CE
Dean
Ralph A
RA
D000595:Amino Acid Sequence; D022202:Chromosomes, Artificial, Bacterial; D020451:Contig Mapping; D028463:Cucumis melo / Q000235:genetics* / Q000382:microbiology; D005670:Fusarium; D015723:Gene Library; D017343:Genes, Plant; D005819:Genetic Markers; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics*; D016415:Sequence Alignment
D005819:Genetic Markers