title: [Detection of the endotoxin of Gram-negative bacteria in human blood].
abstract: Recent new data on the important role played by lipopolysaccharides (endotoxin) of Gram negative bacteria in physiology and pathogenesis of the most important human infectious and noninfectious diseases testify to the necessity of wide clinical trials of different methods for LPS detection in blood and other physiological fluids. Among presently available diagnostic methods for endotoxinemia detection, the highly sensitive LAL (Limulus Amebocyte Lysate) test in various modifications is most widely used. The LAL test is known to be non-specific, however many drawbacks of this test have been successfully overcome. The results of clinical studies on the determination of the LPS activity in the systemic blood stream and antibody titers to its most common determinants, as well as the reserves of endotoxin binding with granulocytes give grounds for optimistic evaluation of the future studies on the role of LPS in human physiology and pathology. In clinical practice both positive sides and drawbacks of the presently known methods for LPS detection, including the LAL test, must be borne in mind for the complex evaluation of endotoxinemia levels.
authors.affiliation: Gamaleya Research Institute of Epidemiology and Microbiology, Institute of General and Clinical Pathology, Moscow, Russia.
authors.forename: V M
authors.initials: VM
authors.lastname: Bondarenko
authors.forename: V G
authors.initials: VG
authors.lastname: Likhoded
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Iakovlev
mesh_terms: D001769:Blood / Q000382:microbiology*; D004731:Endotoxins / Q000097:blood* / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D016905:Gram-Negative Bacterial Infections / Q000097:blood*; D006098:Granulocytes / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000097:blood* / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides