Prevalence of HIV co-receptor polymorphisms in HIV-infected patients and uninfected volunteers in Luxembourg.
OBJECTIVE
To describe the prevalence of nine HIV-1 co-receptor polymorphisms in Luxembourg (CCR5-Delta 32, CCR5-58755-A/G, CCR5m303, CCR5-59029-A/G, CCR2-64I, CCR5-59653-C/T, CX(3) CR1-V249I, CX(3) CR1-T280M, and SDF1-3'A) in 288 HIV-1-infected patients and in 158 uninfected, healthy volunteers.


METHODS
The presence of mutations was detected using PCR-restriction fragment length polymorphism (RFLP)-based methods.


RESULTS
We did not find any significant differences in genotype distributions and allele frequencies between infected and uninfected participants in Luxembourg. The distribution of genotypes agreed in all groups with Hardy-Weinberg predicted proportions. CCR5-Delta 32 was significantly associated with HIV-1-infected slow progressor patients.


CONCLUSIONS
Overall, allele frequencies were comparable to frequencies reported in previous studies in Caucasian populations.
Roman
François
F
Laboratoire de Rétrovirologie, Centre de Recherche Public - Santé, Centre Hospitalier de Luxembourg, Luxembourg. roman.f@retrovirology.lu
Franck
Nathalie
N
Burgy
Catherine
C
Servais
Jean
J
Zimmer
Jean-Marie
JM
Mossong
Joël
J
Goubau
Patrick
P
Schneider
François
F
Hemmer
Robert
R
Schmit
Jean-Claude
JC
D044466:Asian People / Q000235:genetics; D044383:Black People / Q000235:genetics; D015331:Cohort Studies; D005858:Germany / Q000453:epidemiology; D015658:HIV Infections / Q000453:epidemiology* / Q000235:genetics; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D019713:Receptors, CCR5 / Q000235:genetics*; D044465:White People / Q000235:genetics
D019713:Receptors, CCR5