title: Studies on mass production of transformed Panax ginseng hairy roots in bioreactor.
abstract: The growth properties of Panax ginseng hairy roots transformed by Agrobacterium rhizogenes were compared between flask and aerated column or stirred bioreactor. In flask cultures, sucrose, initially 30 g/L, was nearly exhausted after 45 d of culture. The pH of the medium dropped from 5.5 to 4.96 after 10 d, but afterward it gradually increased to 6.4. After 45 d, hairy roots grew about 16-folds. The growth rate of hairy roots in air-bubble column or stirred bioreactor cultures was 1.13 (1.11) to 1.23 (1.20) g fresh wt (dry wt)/(g of cells x d), respectively. For both bioreactors, growth was about three times as high as in the flask cultivation.
authors.affiliation: Faculty of Chemical Engineering, Chonnam National University, Kwangju, Korea.
authors.forename: Gwi-Taek
authors.initials: GT
authors.lastname: Jeong
authors.forename: Don-Hee
authors.initials: DH
authors.lastname: Park
authors.forename: Baik
authors.initials: B
authors.lastname: Hwang
authors.forename: Kyungmoon
authors.initials: K
authors.lastname: Park
authors.forename: Si-Wouk
authors.initials: SW
authors.lastname: Kim
authors.forename: Je-Chang
authors.initials: JC
authors.lastname: Woo
mesh_terms: D000388:Air; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D002478:Cells, Cultured; D007700:Kinetics; D005894:Panax / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D012231:Rhizobium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012503:Saponins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D012503:Saponins