title: A new method for fixing Biomphalaria glabrata for histologic studies, using shell perforation.
abstract: A new technique for fixation of Biomphalaria glabrata for histologic studies is described. It consists in performing several external holes in the shell, before placing the entire snail into the fixative. It is a very practical and quick procedure that showed excellent results when compared to the usual techniques.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: Luciene
authors.initials: L
authors.lastname: Barbosa
authors.forename: Paulo Marcos Z
authors.initials: PM
authors.lastname: Coelho
authors.forename: Débora
authors.initials: D
authors.lastname: Negrão-Corrêa
authors.forename: Zilton A
authors.initials: ZA
authors.lastname: Andrade
mesh_terms: D000818:Animals; D001700:Biomphalaria; D004396:Coloring Agents; D006416:Hematoxylin; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D004396:Coloring Agents; D006416:Hematoxylin