Origin of two isoprenoid units in a lavandulyl moiety of sophoraflavanone G from Sophora flavescens cultured cells.
Cell suspension cultures of Sophora flavescens produced large amounts of sophoraflavanone G, an 8-lavandulylated flavanone and lupalbigenin, a 6,3'-di-dimethylallylated isoflavone, by the simultaneous addition of cork tissues and methyl jasmonate. The labeling pattern of the isoprene units resulting after administration of [1-13C] glucose into the cell cultures in the presence of the above additives revealed that two isoprene units in the lavandulyl group of sophoraflavanone G and two dimethylallyl groups of lupalbigenin were biosynthesized via the 1-deoxy-D-xylulose-5-phosphate pathway.
Yamamoto
Hirobumi
H
Medicinal Plant Garden, School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Japan. hirobumi@net.nagasaki-u.ac.jp
Zhao
Ping
P
Inoue
Kenichiro
K
D002070:Butadienes / Q000378:metabolism; D002247:Carbon Isotopes; D002478:Cells, Cultured; D044950:Flavanones; D005419:Flavonoids / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism; D045782:Hemiterpenes; D010420:Pentanes; D017368:Protein Prenylation; D029909:Sophora / Q000166:cytology / Q000378:metabolism*
D002070:Butadienes; D002247:Carbon Isotopes; D044950:Flavanones; D005419:Flavonoids; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene; C064849:vexibinol; D005947:Glucose