[Determination of angiotensin-converting enzyme activity using dansyl-Phe-Ala-Arg as the substrate].
Modificated method for the determination of the angiotensin converting enzyme (EC 3.4.15.1) activity in neural tissue is proposed. The methods is based on fluorimetrically determination of released dansyl-Phe from dansyl-Phe-Ala-Arg at pH 7.6. Km is 50 +/- 10 MM. The high specificity of the method is provide by using captopril, the high specific angiotensin converting enzyme inhibitor. The sensitivity of the method is 0.001 nmol/min per mg protein that it is lesser in 15-fold than the lowest enzyme activity in brain region. The distribution of the angiotensin converting enzyme activity that using the method in brain regions and peripherial tissues of rat is presented.
Shchetinina
N V
NV
Penza State Pedagogical University, Russia.
Vernigora
A N
AN
Gengin
M T
MT
D000818:Animals; D001921:Brain / Q000201:enzymology; D003619:Dansyl Compounds / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009842:Oligopeptides / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D051381:Rats; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
D003619:Dansyl Compounds; D009842:Oligopeptides; C043048:dansyl-phenylalanyl-leucyl-arginine; D007703:Peptidyl-Dipeptidase A