title: Cowpea mosaic virus 32- and 60-kilodalton replication proteins target and change the morphology of endoplasmic reticulum membranes.
abstract: Cowpea mosaic virus (CPMV) replicates in close association with small membranous vesicles that are formed by rearrangements of intracellular membranes. To determine which of the viral proteins are responsible for the rearrangements of membranes and the attachment of the replication complex, we have expressed individual CPMV proteins encoded by RNA1 in cowpea protoplasts by transient expression and in Nicotiana benthamiana plants by using the tobacco rattle virus (TRV) expression vector. The 32-kDa protein (32K) and 60K, when expressed individually, accumulate in only low amounts but are found associated with membranes mainly derived from the endoplasmic reticulum (ER). 24K and 110K are freely soluble and accumulate to high levels. With the TRV vector, expression of 32K and 60K results in rearrangement of ER membranes. Besides, expression of 32K and 60K results in necrosis of the inoculated N. benthamiana leaves, suggesting that 32K and 60K are cytotoxic proteins. On the other hand, during CPMV infection 32K and 60K accumulate to high levels without causing necrosis.
authors.affiliation: Laboratory of Molecular Biology, Wageningen University, Wageningen, The Netherlands.
authors.forename: Jan E
authors.initials: JE
authors.lastname: Carette
authors.forename: Jan
authors.initials: J
authors.lastname: van Lent
authors.forename: Stuart A
authors.initials: SA
authors.lastname: MacFarlane
authors.forename: Joan
authors.initials: J
authors.lastname: Wellink
authors.forename: Ab
authors.initials: A
authors.lastname: van Kammen
mesh_terms: D017798:Comovirus / Q000378:metabolism / Q000472:pathogenicity*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D007887:Fabaceae / Q000821:virology*; D007425:Intracellular Membranes / Q000378:metabolism*; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D011523:Protoplasts / Q000821:virology; D012329:RNA, Bacterial / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism; D014026:Nicotiana / Q000821:virology; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: C042027:RNA I; D012329:RNA, Bacterial; D014764:Viral Proteins