Gene expression during Pi deficiency in Pholiota nameko: accumulation of mRNAs for two transporters.
The effects of Pi deficiency on gene expression in Pholiota nameko were examined. A cDNA library was constructed from poly(A)+ RNA isolated from mycelia cultured in Pi-depleted (P-) media, and 150 clones corresponding to Pi deficiency-inducible (pdi) genes were selected by differential hybridization with probes prepared from poly(A)+ RNAs from the mycelia cultured in the Pi-supplied (P+) and P- media. These clones were considered to derive from 31 genes by cross-hybridization. Northern blot analysis showed that these pdi genes were expressed in various patterns during Pi deficiency. Among the clones, the DNA sequences of pdi85 and pdi343 were analyzed. The deduced amino acid sequences indicated that they have structural similarities to Pi and metabolite transporters.
Tasaki
Yuji
Y
Graduate School of Science and Technology, Niigata University, Ikarashi, Japan.
Kamiya
Yuki
Y
Azwan
A
A
Hara
Takashi
T
Joh
Toshio
T
D000363:Agaricales / Q000235:genetics* / Q000254:growth & development; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism*; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D010710:Phosphates; D012331:RNA, Fungal; D012333:RNA, Messenger