Transformed bacterial symbionts re-introduced to and detected in host gut.
Strains of Enterobacter agglomerans and Klebsiella pneumoniae isolated from Rhagoletis completa Cresson were engineered to express transgenic fluorescent proteins (ECFP, DsRed). These bacteria were introduced into flies by feeding the flies a sucrose solution in which the bacteria were suspended. The transgenic and heterologous marker protein was expressed and visible in the bacteria after they were ingested by WHF and while they were in the fly gut. We describe the plasmids used to transform these bacteria and demonstrate expression of heterologous proteins from the transforming plasmids and discuss the implications for future pest control strategies.
Peloquin
John J
JJ
Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA. Peloquin@faculty.ucr.edu
Lauzon
Carol R
CR
Potter
Sarah
S
Miller
Thomas A
TA
D000818:Animals; D001761:Bleomycin / Q000737:chemistry; D004064:Digestive System / Q000382:microbiology; D004175:Diptera / Q000382:microbiology*; D004754:Enterobacter / Q000235:genetics / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000235:genetics* / Q000302:isolation & purification*; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification; D008164:Luminescent Proteins / Q000032:analysis; D010957:Plasmids / Q000235:genetics; D013559:Symbiosis; D014169:Transformation, Bacterial; D019076:Transgenes
D008164:Luminescent Proteins; C413663:fluorescent protein 583; D001761:Bleomycin; C105427:Zeocin