title: Multicenter Brazilian study of oral Candida species isolated from AIDS patients.
abstract: Oropharyngeal candidiasis continues to be considered the most common opportunistic disease in Aids patients. This study was designed to investigate species distribution, serotype and antifungal susceptibility profile among Candida spp. isolated from the oral cavity of Aids patients recruited from six Brazilian university centers. Oral swabs from 130 Aids patients were plated onto CHROMagar Candida medium and 142 isolates were recovered. Yeast isolates were identified by classical methods and serotyped using the Candida Check or target system-Iatron. Antifungal susceptibility testing was performed according to the NCCLS microbroth assay. C. albicans was the most frequently isolated species (91%), and 70% of the isolates belonged to serotype A. We detected 12 episodes of co-infection (9%), including co-infection with both serotypes of C. albicans. Non-albicans species were isolated from 12 episodes, 50% of them exhibited DDS or resistance to azoles. Otherwise, only 8 out 130 isolates of C. albicans exhibited DDS or resistance to azoles. Brazilian Aids patients are infected mainly by C. albicans serotype A, most of them susceptible to all antifungal drugs.
authors.affiliation: Laboratório Especial de Micologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, 04012-037, Brasil.
authors.forename: Priscilla de Laet
authors.initials: Pde L
authors.lastname: Sant'Ana
authors.forename: Eveline Pípolo
authors.initials: EP
authors.lastname: Milan
authors.forename: Roberto
authors.initials: R
authors.lastname: Martinez
authors.forename: Flávio
authors.initials: F
authors.lastname: Queiroz-Telles
authors.forename: Marcelo Simão
authors.initials: MS
authors.lastname: Ferreira
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Alcântara
authors.forename: Miriam T
authors.initials: MT
authors.lastname: Carvalho
authors.forename: Arnaldo Lopes
authors.initials: AL
authors.lastname: Colombo
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000382:microbiology*; D025141:Drug Resistance, Fungal; D005260:Female; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D007654:Ketoconazole / Q000494:pharmacology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D011446:Prospective Studies; D012703:Serotyping
chemical_list: D000935:Antifungal Agents; D017964:Itraconazole; D015725:Fluconazole; D007654:Ketoconazole