Precautions in the use of 110mAg as a tracer of silver metabolism in ecotoxicology: preferential bioconcentration of 109Cd by trout gills after 110mAg exposure.
An often overlooked problem in the use of radiotracers is the possibility of isotopic contamination. Commercially available silver 110mAg was used to study silver uptake and depuration in rainbow trout and European eel. Quality control by means of comparative gamma and beta counting brought our attention to a contamination of the 110mAg stock with 109Cd, which could be seen only because the 109Cd was markedly bioconcentrated by trout gills. The contamination could not be detected in eel gills or in other tissues of both species. The difference between trout and eel gill structure and function is the probable explanation for the marked difference in 109Cd accumulation. This contamination was identified as 109Cd by gamma spectroscopy and its origin by transmutation of natural silver as a result of neutron activation is described. Failure to recognize this contamination problem would have resulted in serious misinterpretation of the data set. Guidance for avoiding this problem is given.
Hansen
Heinz J M
HJ
Risoe National Laboratory, Nuclear Department, Roskilde, Denmark.
Grosell
Martin
M
Jacobsen
Ulf
U
Jørgensen
Jesper C
JC
Hogstrand
Christer
C
Wood
Christopher M
CM
D000818:Animals; D002106:Cadmium Radioisotopes / Q000302:isolation & purification*; D004524:Eels; D004781:Environmental Exposure; D005880:Gills / Q000502:physiology; D017686:Oncorhynchus mykiss / Q000502:physiology; D011786:Quality Control; D011868:Radioisotopes / Q000737:chemistry* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry* / Q000493:pharmacokinetics; D013057:Spectrum Analysis; D018675:Toxicity Tests
D002106:Cadmium Radioisotopes; D011868:Radioisotopes; D012834:Silver