[Effects of isorhapotigenin and resveratrol on function of peripheral blood polymorphonuclear leukocytes from rabbits].
In effects of isorhapotigenin and resveratrol on chemotaxis and release of beta-glucuronidase from rabbit peripheral blood polymorphonuclear leukocytes stimulated with formylmethionyl-leucyl-phenyl-phenylalanine (fMLPP) were studied. The optimal concentration of fMLPP for the induction of chemotaxis of polymorphonuclear leukocytes was approximately at 5 x 10(-10) mol.L-1. fMLPP, at the concentration range of 10(-10)-10(-6) mol.L-1, dose-dependently increased the release of beta-glucuronidase from polymorphonuclear leukocytes. Isorhapotigenin and resveratrol both inhibited the chemotaxis of polymorphonuclear leukocytes stimulated with fMLPP(5 x 10(-10) mol.L-1). Isorhapotigenin, at the concentration range of 10(-5)-10(-4) mol.L-1, dose-dependently inhibited the release of beta-glucuronidase from polymorphonuclear leukocytes stimulated with fMLPP(10(-8) mol.L-1). Resveratrol, at the concentrations of 1 x 10(-5) and 3 x 10(-5) mol.L-1, was shown to dose-dependently inhibit beta-glucuronidase release from polymorphonuclear leukocytes stimulated with fMLPP(10(-8) mol.L-1).
Zhong
M
M
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050.
Guo
Y
Y
Deng
J
J
Wang
W
W
Cheng
G
G
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D005966:Glucuronidase / Q000097:blood; D009504:Neutrophils / Q000201:enzymology / Q000502:physiology; D011817:Rabbits; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D013267:Stilbenes; C432307:isorhapontigenin; D005966:Glucuronidase; D000077185:Resveratrol