Cellular immunolocalization of gastric and pancreatic lipase in various tissues obtained from dogs.
OBJECTIVE
To determine cellular immunolocalization of canine gastric lipase (cGL) and canine pancreatic lipase (cPL) in various tissues obtained from clinically healthy dogs.


METHODS
Samples of 38 tissues collected from 2 climically healthy dogs.


METHODS
The cGL and cPL were purified from gastric and pancreatic tissue, respectively, obtained from dogs. Antisera against both proteins were developed, using rabbits, and polyclonal antibodies were purified by use of affinity chromatography. Various tissues were collected from 2 healthy dogs. Primary antibodies were used to evaluate histologic specificity. Replicate sections from the collected tissues were immunolabeled for cGL and cPL and examined by use of light microscopy.


RESULTS
Mucous neck cells and mucous pit cells of gastric glands had positive labeling for cGL, whereas other tissues did not immunoreact with cGL. Pancreatic acinar cells had positive labeling for cPL, whereas other tissues did not immunoreact with cPL.


CONCLUSIONS
We concluded that cGL and cPL are exclusively expressed in gastric glands and pancreatic acinar cells, respectively. Also, evidence for cross-immunoreactivity with other lipases or related proteins expressed by other tissues was not found for either protein. Analysis of these data suggests that gastric lipase is a specific marker for gastric glands and that pancreatic lipase is a specific marker for pancreatic acinar cells. These markers may have clinical use in the diagnosis of gastric and exocrine pancreatic disorders, respectively.
Steiner
Jörg M
JM
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station 77843-4474, USA.
Berridge
Brian R
BR
Wojcieszyn
John
J
Williams
David A
DA
D000818:Animals; D004285:Dogs / Q000378:metabolism*; D007150:Immunohistochemistry / Q000662:veterinary; D008049:Lipase / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D013270:Stomach / Q000201:enzymology*
D008049:Lipase