Biochemical properties of a keratan sulphate/chondroitin sulphate proteoglycan expressed in primate pluripotent stem cells.
We previously identified a pericellular matrix keratan sulphate/chondroitin sulphate proteoglycan present on the surface of human embryonal carcinoma stem cells, cells whose differentiation mimics early development. Antibodies reactive with various epitopes on this molecule define a cluster of differentiation markers for primate pluripotent stem cells. We describe the purification of a form of this molecule which is secreted or shed into the culture medium. Biochemical analysis of the secreted form of this molecule shows that the monomeric form, whilst containing keratan sulphate, resembles mucins in its structure and its modification with O-linked carbohydrate. Immunofluorescence and immunoblotting data show that monkey and human pluripotent stem cells react with antibodies directed against epitopes on either carbohydrate side chains or the protein core of the molecule.
Cooper
Susan
S
University College London, UK.
Bennett
William
W
Andrade
Jessica
J
Reubinoff
Benjamin E
BE
Thomson
James
J
Pera
Martin F
MF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D017077:Culture Media, Conditioned; D004622:Embryo, Mammalian / Q000166:cytology*; D006031:Glycosylation; D006801:Humans; D007632:Keratan Sulfate / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D000071234:Lumican; D008253:Macaca mulatta; D012995:Solubility; D013234:Stem Cells / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D011508:Chondroitin Sulfate Proteoglycans; D017077:Culture Media, Conditioned; C000604306:LUM protein, human; D000071234:Lumican; D007632:Keratan Sulfate