High-performance liquid chromatographic analysis and separation of N-feruloylserotonin isomers.
The N-feruloylserotonin containing fraction was isolated from seeds of Leuzea carthamoides (Willd.) DC by solvent extraction followed by column chromatography on silica gel or on Sephadex LH-20. Nuclear magnetic resonance spectroscopic analysis of the isolated fraction showed the presence of four structurally related compounds. These compounds were identified as four isomers of N-feruloylserotonin: N-(Z)-feruloylserotonin, N-(Z)-isoferuloylserotonin, N-(E)-feruloylserotonin and N-(E)-isoferuloylserotonin. They were analyzed by HPLC on Separon SGX C18, Separon SGX and Separon SGX phenyl, using various mobile phases. Separon SGX phenyl phase was found the most efficient for a rapid analysis and for the final separation of the N-feruloylserotonin isomers.
Pavlík
Milan
M
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague.
Laudová
Vera
V
Grüner
Karel
K
Vokác
Karel
K
Harmatha
Juraj
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007536:Isomerism; D012701:Serotonin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D012701:Serotonin