title: Phototrophic transformation of phenol to 4-hydroxyphenylacetate by Rhodopseudomonas palustris.
abstract: Newly isolated and culture collection strains of Rhodopseudomonas palustris were able to transform phenol to 4-hydroxyphenylacetate under phototrophic conditions in the presence of acetate, malate, benzoate, or cinnamate as growth substrates. The reaction was examined with uniformly (14)C-labelled phenol and the product was identified by HPLC retention time, UV-scans, and (1)H- and (13)C-NMR analysis. The transformation reaction was detectable in cell-free extracts in the presence of NAD(+) and acetyl-CoA. For further degradation of 4-hydroxyphenylacetate by R. palustris, low partial pressures of oxygen were essential, presumably for aerobic aromatic ring fission reactions by mono- and di-oxygenases.
authors.affiliation: Applied Microbiology, University of Saarbrücken, PO Box 151150, 66041 Saarbrücken, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Noh
authors.forename: S
authors.initials: S
authors.lastname: Heck
authors.forename: F
authors.initials: F
authors.lastname: Giffhorn
authors.forename: G-W
authors.initials: GW
authors.lastname: Kohring
mesh_terms: D009682:Magnetic Resonance Spectroscopy; D010100:Oxygen / Q000378:metabolism; D019800:Phenol / Q000378:metabolism*; D010648:Phenylacetates / Q000378:metabolism*; D012241:Rhodopseudomonas / Q000378:metabolism*
chemical_list: D010648:Phenylacetates; C026246:4-hydroxyphenylacetate; D019800:Phenol; D010100:Oxygen