Production of antibodies against glycolipids from the Mycobacterium tuberculosis cell wall in aerosol murine models of tuberculosis.
Evolution of antibodies against glycolipids from the Mycobacterium tuberculosis cell wall has been studied for the first time in experimental murine models of tuberculosis induced by aerosol, in which infection, reinfection, reactivation, prophylaxis and treatment with antibiotics have been assayed. Results show a significant humoral response against these antigens, where diacyltrehaloses (DAT) and sulpholipid I (SL-I) elicited higher antibody levels than protein antigens like antigen 85 protein complex (Ag85), culture filtrate proteins (CFP) and purified protein derivative (PPD). Only immunoglobulin M (IgM) antibodies have been detected against DAT and SL-I. Their evolution has a positive correlation with bacillary concentration in tissues.
Cardona
P J
PJ
Unitat de Tuberculosi Experimental, Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Badalona, Catalonia, Spain. pcardona@ns.hugtip.scs.es
Julián
E
E
Vallès
X
X
Gordillo
S
S
Muñoz
M
M
Luquin
M
M
Ausina
V
V
D000217:Acyltransferases; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis; D004187:Disaccharides / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D006017:Glycolipids / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D014373:Tuberculin / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology*; D037521:Virulence Factors
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; D016207:Cytokines; D004187:Disaccharides; D006017:Glycolipids; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D014373:Tuberculin; D037521:Virulence Factors; C009355:sulfolipid I; D000217:Acyltransferases; C112580:antigen 85A, Mycobacterium tuberculosis; C042003:trehalulose