Partial characterization of a human submandibular/sublingual salivary adhesion-promoting protein.
Human submandibular/sublingual saliva contains a protein that promotes adhesion of Streptococcus mutans JBP serotype-c to spheroidal hydroxyapatite in vitro. A high molecular-weight (250,000-300,000 Da) adhesion-promoting protein (APP) was purified by Trisacryl 2000 M gel-filtration chromatography and gel electroelution before it was partially characterized. Lectin blotting identified that the terminal carbohydrates include N-acetyl glucosamine-beta 1-4-N-acetylglucosamine, galactose and galactose-beta 1-3-N-acetyl galactosamine. Antibodies to APP demonstrated no difference in the immunoreactive pattern of APP from saliva of caries-active or caries-resistant individuals belonging to four different ethnic groups: Asian, African-American, Hispanic or Caucasian. No immunological similarities to salivary mucins or parotid agglutinins were detected by Western blotting using immuno-cross-reactivity as a criterion. APP appears to be a unique protein found in submandibular/sublingual saliva. Understanding such a protein could help prevent S. mutans attachment to the enamel surface.
Akintoye
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SO
Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 E 24th Street, New York, NY 10010-4086, USA.
Dasso
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M
Hay
D I
DI
Ganeshkumar
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N
Spielman
A I
AI
D001422:Bacterial Adhesion; D015153:Blotting, Western; D002850:Chromatography, Gel; D003731:Dental Caries / Q000382:microbiology; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007150:Immunohistochemistry; D008970:Molecular Weight; D012471:Salivary Proteins and Peptides / Q000737:chemistry* / Q000302:isolation & purification; D013295:Streptococcus mutans / Q000502:physiology; D013363:Submandibular Gland / Q000378:metabolism
D012471:Salivary Proteins and Peptides; C465005:salivary adhesion-promoting protein, human; D017886:Durapatite