Folding and intrinsic stability of deletion variants of PrP(121-231), the folded C-terminal domain of the prion protein.
Transmissible spongiform encephalopathies in mammals are believed to be caused by PrPSc, the insoluble, oligomeric isoform of the cellular prion protein PrPC. PrPC and the subunits of PrPSc have identical covalent but different tertiary structure. To address the question of whether parts of the structure of PrPC are sufficiently stable to be retained in PrPSc, we have constructed two deletion variants of the C-terminal PrPC domain, PrP(121-231), which is the only part of recombinant PrP with defined tertiary structure. One of the variants, H2-H3, comprises the last two alpha-helices of PrP(121-231) that have been proposed to be preserved in models of PrP(Sc). In the other variant, PrP(121-231)-deltaH1, the first alpha-helix of PrP(121-231) was deleted and replaced by introduction of the beta-turn dipeptide Asn-Gly between the strands of the single beta-sheet of PrP(121-231). Although both deletion constructs still show alpha-helical CD-spectra, they are more disordered and thermodynamically strongly destabilized compared to PrP(121-231), with free energies of folding close to zero. These data demonstrate that the tertiary structure context is critical for the conformation of the segment comprising alpha-helix 2 and 3 in the solution structure of recombinant PrP.
Eberl
Heike
H
Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Hönggerberg, Zürich, Switzerland.
Glockshuber
Rudi
R
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D051379:Mice; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics; D010749:Phosphoprotein Phosphatases / Q000737:chemistry; D018620:PrPSc Proteins / Q000737:chemistry; D011328:Prions / Q000737:chemistry* / Q000235:genetics; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017384:Sequence Deletion; D014508:Urea / Q000494:pharmacology
D001426:Bacterial Proteins; D010446:Peptide Fragments; D018620:PrPSc Proteins; D011328:Prions; C100569:prion protein (121-231); D014508:Urea; D010749:Phosphoprotein Phosphatases