title: [Detection of inv (16) in acute myelomonocytic leukemia by interphase fluorescence in situ hybridization].
abstract: OBJECTIVE
  To evaluate fluorescence in situ hybridization (FISH) in the detection of inv (16) (p13; q22).
  
  
  METHODS
  Spectrum red labeled yeast artificial chromosome (YAC) clone 854E2 which spans the breakpoint cluster region in MYH11 in band 16p13 and single color interphase FISH were used to detect inv (16) in 26 cases of acute myelomonocytic leukemias (AML-M(4)), and the results were compared with that of conventional cytogenetic analysis.
  
  
  RESULTS
  R banding karyotyping test revealed no inv (16) in 25 cases, one AML M(4Eo) case showed inv (16) by G banding. Nine cases including all three M(4Eo) had inv (16) by FISH analysis, among whom the characteristic fluorescence signal pattern of the inv (16) was seen in 13.3% to 32.1% (median, 21.3%) of the tested cells.
  
  
  CONCLUSIONS
  YAC 854E2 and interphase FISH provide a powerful technique in the detection of inv (16) (p13q22).
authors.affiliation: First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou 215006, China.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Li
authors.forename: Jinlan
authors.initials: J
authors.lastname: Pan
authors.forename: Yafang
authors.initials: Y
authors.lastname: Wu
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Xue
mesh_terms: D000328:Adult; D000368:Aged; D007446:Chromosome Inversion; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase / Q000235:genetics; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged