Crystallization and preliminary X-ray crystallographic analysis of enoyl-acyl carrier protein reductase from Helicobacter pylori.
Enoyl-acyl carrier protein reductase (ENR) catalyzes the NADH-dependent stereospecific reduction of alpha,beta-unsaturated fatty acids bound to the acyl-carrier protein. ENR from Helicobacter pylori has been overexpressed in Escherichia coli and has been crystallized in the presence of its cofactor NADH and the inhibitor triclosan (or its analogue diclosan) at 296 K using polyethylene glycol (PEG) 400 as a precipitant. For the triclosan (or diclosan) complex, diffraction data to 2.5 (or 2.3) A resolution have been collected using synchrotron X-rays. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 73.35, b = 94.91, c = 75.38 A, beta = 106.21 degrees for the triclosan complex (or a = 73.25, b = 95.07, c = 75.02 A, beta = 106.53 degrees for the diclosan complex). The asymmetric unit contains one homotetramer, with a corresponding V(M) of 2.10 A(3) Da(-1) and a solvent content of 41% by volume.
Lee
Hyung Ho
HH
Laboratory of Structural Proteomics, School of Chemistry and Molecular Engineering, Seoul National University, Seoul 151-742, South Korea.
Yun
Jungmin
J
Moon
Jinho
J
Han
Byung Woo
BW
Lee
Byung Il
BI
Lee
Jae Young
JY
Suh
Se Won
SW
D003460:Crystallization; D018360:Crystallography, X-Ray; D050753:Enoyl-(Acyl-Carrier-Protein) Reductase (NADH); D004791:Enzyme Inhibitors; D016480:Helicobacter pylori / Q000201:enzymology*; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000737:chemistry*; D011487:Protein Conformation; D014260:Triclosan / Q000494:pharmacology
D004791:Enzyme Inhibitors; D014260:Triclosan; D010088:Oxidoreductases; D050753:Enoyl-(Acyl-Carrier-Protein) Reductase (NADH)