IR spectra and IR spectral maps of individual normal and cancerous cells.
The IR microspectra for individual normal and cancerous cells are reported at a spatial resolution that permits a distinction between the nuclear and cytoplasmic regions. The observed spectra reveal large differences in the spectral contributions of RNA, DNA, and phospholipids: metabolically inactive cells show spectral patterns of proteins only, whereas actively dividing cells also show spectral signatures of nucleic acids and phospholipids. These spectral variations are independent of the state of health of a cell.
Diem
Max
M
Department of Chemistry, City University of New York, Hunter College, 695 Park Avenue, New York, New York 10021, USA. mdiemhc@aol.com
Chiriboga
Luis
L
Lasch
Peter
P
Pacifico
Anthony
A
D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D016193:G1 Phase; D016195:G2 Phase; D015726:Giant Cells / Q000378:metabolism; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D016196:S Phase; D012509:Sarcoma / Q000378:metabolism; D013055:Spectrophotometry, Infrared / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism
D010743:Phospholipids; D012313:RNA; D004247:DNA