[Experimental study on repair of peripheral nerve defect by basic fibroblast growth factor combined with autogenous vein graft conduit].
OBJECTIVE
To explore the effect of basic fibroblast growth factor (bFGF) combined with autogenous vein graft conduit on peripheral nerve regeneration.


METHODS
Fifty four New Zealand rabbits were divided into three groups. The main trunk of sciatic nerve of rabbit in one side was severed and bridged by autogenous vein. 0.2 ml bFGF solution (4,000 U/ml) was intravenously injected to the vein graft conduit as group A, the same amount of saline solution as group B, and no solution injection as group C. Microscopic examination, axon video analysis and nerve conduct velocity were performed at the 10th, 30th, and 100th day after operation.


RESULTS
The nerve fibers were grown into vein graft conduit in all groups at 30th after operation, they were more and regular in group A than that of group B and C, and the axon regeneration rate in group A was more than that of group B and C.


CONCLUSIONS
bFGF combined with autogenous vein graft conduit can markedly promote nerve regeneration.
Li
C Y
CY
Department of Hand Surgery, Bao'an People's Hospital, Shenzhen Guangdong, P. R. China 518101.
Cao
D C
DC
D000818:Animals; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D007601:Jugular Veins / Q000637:transplantation*; D008297:Male; D009416:Nerve Regeneration; D059348:Peripheral Nerve Injuries; D011817:Rabbits; D011897:Random Allocation; D012584:Sciatic Nerve / Q000293:injuries* / Q000601:surgery
D016222:Fibroblast Growth Factor 2