ER function in the adult male rat: short- and long-term effects of the antiestrogen ICI 182,780 on the testis and efferent ductules, without changes in testosterone.
Male rats, 30 d old, were treated with the antiestrogen ICI 182,780 (3-150 d) to determine sequences of events leading to testicular atrophy and infertility. Plasma testosterone and LH concentrations were unchanged. ICI 182,780 induced dilation of efferent ductules as early as 3 d post treatment, and the dilation increased over time, resulting in an overall increase of 200% in tubule diameter. A gradual reduction in height of the ductule epithelium was observed; however, the microvilli height increased up to d 73 but subsequently decreased. A transient increase in lysosomes in nonciliated cells was seen from d 15 to d 100. Testicular weight increased by d 45 and seminiferous tubules were dilated by d 52. These effects on testes persisted until d 100, but on d 150 the weight decreased and severe atrophy was observed. These testicular effects were probably owing to accumulation of fluid following inhibition of reabsorption in the efferent ductules, similar to the ER-alpha knockout mouse. In agreement with this conclusion, there was a decrease in Na+-H+ exchanger-3 mRNA and protein, which is consistent with previous studies showing that ER is required for expression of Na+-H+ exchanger-3 and ultimately fluid reabsorption in the efferent ductules.
Oliveira
Cleida A
CA
Department of Veterinary Biosciences, University of Illinois, Urbana, Illinois 61802, USA.
Zhou
Qing
Q
Carnes
Kay
K
Nie
Rong
R
Kuehl
David E
DE
Jackson
Gary L
GL
Franca
Luiz R
LR
Nakai
Masaaki
M
Hess
Rex A
RA
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001284:Atrophy; D015152:Blotting, Northern; D001835:Body Weight / Q000187:drug effects; D004543:Ejaculatory Ducts / Q000187:drug effects* / Q000201:enzymology; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D000077267:Fulvestrant; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007150:Immunohistochemistry; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000502:physiology*; D012671:Seminiferous Tubules / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers / Q000096:biosynthesis / Q000235:genetics; D013737:Testis / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D013739:Testosterone / Q000097:blood*
D004965:Estrogen Antagonists; D011960:Receptors, Estrogen; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers; D000077267:Fulvestrant; D013739:Testosterone; D004958:Estradiol; D000135:Acid Phosphatase