[Composition analysis of hematopoietic stem/progenitor cells in peripheral blood and bone marrow from patients with paroxysmal nocturnal hemoglobinuria].
OBJECTIVE
To evaluate the composition and proportion of the hematopoietic stem cells in peripheral blood (PB) and bone marrow (BM) in paroxysmal nocturnal hemoglobinuria (PNH) patients.


METHODS
The CD(34) and CD(59) expressions in PB and BM samples from 21 PNH patients and 8 normal volunteers were analyzed by flow cytometry, and their total numbers were compared.


RESULTS
The number of CD(34)(+) cells in both PB and BM of the patients was significantly lower than that in normal controls (P < 0.001), while there was no significant difference between the remittent patients and normal controls. The CD(34)(+) cells in PB of the patients exhibited predominantly normal CD(59) phenotype, which had no relationship either to the patients' clinical manifestations or to the CD(59) expression status of bone marrow CD(34)(+) cells.


CONCLUSIONS
The hematopoietic stem cells in patients with PNH decreases in number but show mainly normal phenotype in PB.
Han
Bing
B
Department of Hematology, Peking Union Medical College, Peking Union Medical College Hospital, CAMS & PUMC, Beijing 100730, China.
Wu
Yongji
Y
Zhang
Zhinan
Z
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology*; D018957:CD59 Antigens / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged
D018952:Antigens, CD34; D018957:CD59 Antigens