Nitric oxide as a regulator of gene expression: studies with the transcription factor proteins cJun and p50.
Both oxidative and nitrosative stresses may result in the inactivation of the binding to DNA of redox-sensitive transcription factors. The underlying biochemical mechanisms may involve oxidation or nitrosylation of critical thiols within the DNA binding domains of these proteins. However, S-glutahionylation, the formation of a mixed disulfide between glutathione and the redox-sensitive cysteine residues, has been shown to occur under NO exposure and pro-oxidative conditions in c-Jun, one of the AP-1 constituents. This modification may be functionally important as it is reversible and has been detected in other transcription factors, such as NF-kappaB, by using covalent chromatography with a modified S-nitrosoglutathione sepharose.
Pineda-Molina
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Centro de Investigaciones Biológicas, Instituto Reina Sofía de Investigaciones Nefrológicas, CSIC, Madrid, Spain.
Lamas
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D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D016328:NF-kappa B / Q000235:genetics*; D052002:NF-kappa B p50 Subunit; D009569:Nitric Oxide / Q000494:pharmacology*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics*
D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D016755:Proto-Oncogene Proteins c-jun; D009569:Nitric Oxide