Storekeeper defines a new class of plant-specific DNA-binding proteins and is a putative regulator of patatin expression.
The expression of class I patatin genes is restricted to potato tubers but can be induced in other tissues by exogenous sucrose. Here we show that tuber-specific and sucrose-inducible gene expression is reduced in transgenic potato plants by mutations in a conserved 10 base pair motif within the B-box of the patatin promoter. In a southwestern screen, we have isolated a novel DNA-binding protein designated Storekeeper (STK) that specifically recognises the B-box motif in vitro. Gel shift experiments with an STK-specific antibody suggest that STK is the B-box binding protein found in tuber nuclei. We propose that STK, the defining member of a new class of DNA binding proteins, regulates patatin expression in potato tubers via the B-box motif.
Zourelidou
Melina
M
Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK.
de Torres-Zabala
Marta
M
Smith
Caroline
C
Bevan
Michael W
MW
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D003001:Cloning, Molecular; D018983:DNA Footprinting / Q000379:methods; D004268:DNA-Binding Proteins / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009154:Mutation; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D018547:Plant Stems / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D011198:Solanum tuberosum / Q000235:genetics*; D013045:Species Specificity; D013395:Sucrose / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics
D004268:DNA-Binding Proteins; D010940:Plant Proteins; D018749:RNA, Plant; D014157:Transcription Factors; C043222:patatin protein, Solanum tuberosum; D013395:Sucrose; D002265:Carboxylic Ester Hydrolases