Non-neuronal cells involved in the degeneration and regeneration of the fish retina.
In the present work we show that during the degenerative process occurring after the cryo-elimination of the tench peripheral growing zone many non-neuronal cell types in addition to the resident microglial cells, appear within the affected areas. Some of them are normally found in the retina, such as the retinal pigmented epithelium cells and others originate from extra-retinal tissues. We identified these as granular leukocytes and macrophages. The microglial cells and macrophages, those resident in the sub-retinal space, and the invasive ones, act as phagocytes. The analysis of the injured retina following lesion shows that the invasive macrophages, arising from the scleral extra-retinal tissues, penetrate the neural retina, and migrate from the scleral to the vitreal portion. In contrast those coming from the vitreal extra-retinal tissues migrate in the opposite direction. Moreover, the retinal pigmented epithelium cells present remarkable modifications in their morphology and distribution and enter the neural retina, where they disrupt the surrounding tissue. We have also observed that this cryo-lesion causes an inflammation mediated by a type of granular leukocyte, denominated heterophils which penetrate the neural retina and probably come from the blood supply. Our results suggest that, during the first days after the lesion, the participation of diverse non-neuronal cells removing cell debris from the damaged zone should create a favourable environment allowing the regeneration of the neural retina.
Lillo
C
C
Biología Celular, INCyL, Facultad de Medicina, Campus Unamuno, Universidad de Salamanca, Spain.
Velasco
A
A
Jimeno
D
D
Cid
E
E
Aijón
J
J
Lara
J M
JM
D000818:Animals; D002465:Cell Movement / Q000502:physiology; D003530:Cyprinidae; D007962:Leukocytes / Q000166:cytology / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure; D008264:Macrophages / Q000473:pathology / Q000502:physiology* / Q000648:ultrastructure; D017628:Microglia / Q000166:cytology* / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D009410:Nerve Degeneration / Q000473:pathology* / Q000503:physiopathology; D009416:Nerve Regeneration; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure; D012160:Retina / Q000166:cytology* / Q000473:pathology / Q000502:physiology* / Q000648:ultrastructure