Effects of streptozotocin-induced diabetes on acetoacetyl-CoA synthetase activity in rats.
In order to investigate the physiological role of acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, EC 6.2.1.16), a cytosolic acetoacetate-activating enzyme, effects of streptozotocin (STZ)-induced diabetes on the enzyme activity was investigated in rats. At 72 hr of the STZ administration (80 mg/kg body weight, injected intravenously), hepatic enzyme specific activity decreased to 23% of its initial activity. However, the enzyme activities in non-hepatic tissues were not significantly affected by the STZ treatment. Feeding of rats with both 4% cholestyramine and 0.4% pravastatin for 3 days remarkably increased the hepatic acetoacetyl-CoA synthetase activity and decreased the plasma ketone bodies level in the diabetic rats. These results suggest that acetoacetyl-CoA synthetase has important roles in the regulation of ketone body utilization in rat liver and that these hypocholesterolemic agents have the ability to remedy the impaired utilization of ketone bodies under the diabetic condition.
Sato
Hiroki
H
Department of Health Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan.
Takahashi
Noriko
N
Nakamoto
Mayumi
M
Ohgami
Masahiro
M
Yamazaki
Masahiro
M
Fukui
Tetsuya
T
D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology; D003066:Coenzyme A Ligases / Q000378:metabolism*; D003920:Diabetes Mellitus / Q000139:chemically induced / Q000201:enzymology*; D004195:Disease Models, Animal; D005260:Female; D007657:Ketone Bodies / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000494:pharmacology; D014018:Tissue Distribution
D000924:Anticholesteremic Agents; D007657:Ketone Bodies; D013311:Streptozocin; D003066:Coenzyme A Ligases; C023747:acetoacetyl-CoA synthetase