[Vitamin D3 and 20-hydroxyecdysone -- inhibitors of free radical lipid oxidation during D-hypervitaminosis].
Under D-hypovitaminosis (control) conditions the statistically reliable increase of blood serum lipids free radical oxidation was revealed in comparison with the intact animals. Administration of vitamin D3 to the animals suffering from D-hypovitaminosis leads for statistically reliable decrease of blood serum lipids free radical oxidation, while 20-hydroxyecdysone in quantity of 0.02 mg per 1 kg of the animal body weight displays the antioxidative properties. Its antioxidative effect is characterized by a statistically reliable increase of Tind chemiluminescence kinetical parameter as compared with the control. Under D-hypovitaminosis conditions in the mitochondrial membranes the products of lipids free radical oxidation--dien conjugates are accumulated. In the case of administrating to the animals suffering from D-hypovitaminosis D3 or 20-hydroxyecdysone these oxidation products are absent. 20-Hydroxyecdysone under these conditions have been revealed as inducing accumulation in the mitochondrial and microsomal membranes of the substances with lambda 225 nm.
Kuz'menko
A I
AI
Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv. akuzm@hotmail.com
Morozova
R P
RP
Nikolenko
I A
IA
Donchenko
G V
GV
D000818:Animals; D002762:Cholecalciferol / Q000494:pharmacology*; D004441:Ecdysterone / Q000494:pharmacology*; D050356:Lipid Metabolism; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D014804:Vitamin B Deficiency / Q000378:metabolism*
D002762:Cholecalciferol; D004441:Ecdysterone