Cloning and analysis of the telomere and terminal inverted repeat of the linear chromosome of Streptomyces griseus.
Cloning and sequencing of the telomere of Streptomyces griseus revealed five palindromic sequences in the terminal 116 nucleotides, all of which can make a hairpin loop structure. However, the end sequence cannot form the foldback secondary structure that is common in Streptomyces telomeres and is suggested to be necessary for terminal replication. Both inside ends of the terminal inverted repeat (TIR) were also cloned and sequenced. The results confirmed the size of the TIR to be 24 kb and identified two almost identical open reading frames that might have been involved in the formation of the TIR.
Goshi
Kohei
K
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.
Uchida
Tetsuya
T
Lezhava
Alexander
A
Yamasaki
Masayuki
M
Hiratsu
Keiichiro
K
Shinkawa
Hidenori
H
Kinashi
Haruyasu
H
D001483:Base Sequence; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013305:Streptomyces griseus / Q000235:genetics*; D016615:Telomere / Q000737:chemistry* / Q000235:genetics*; D020079:Terminal Repeat Sequences / Q000235:genetics*