Isolation and molecular analysis of six taproot expressed genes from sugar beet.
The taproot from sugar beet (Beta vulgaris L.) undergoes a specific developmental process to function as a food storage organ. Suppression Subtractive Hybridization (SSH) was utilized for the isolation of cDNA fragments for taproot expressed genes. Isolation and molecular analysis of six cDNAs encoding the complete gene product revealed that these genes comprise homologues of a drought-inducible linker histone, a homologue of a major latex-like protein, a phosphoenolpyruvate carboxylase kinase, a putative vacuolar processing enzyme, a thaumatin-like protein and an alanine- and glutamic acid-rich protein. All genes are transcribed in taproots while transcription in leaves is low or undetectable.
Kloos
Dorothee U
DU
Institut für Genetik-Biozentrum, Technische Universität Braunschweig, Spielmannstr. 7 D-38106 Braunschweig, Germany.
Oltmanns
Heiko
H
Dock
Christiane
C
Stahl
Dietmar
D
Hehl
Reinhard
R
D027461:Beta vulgaris / Q000235:genetics*; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012333:RNA, Messenger