Detection of genomic alterations in human endometrial cancer by two-dimensional gel electrophoresis.
OBJECTIVE
The purpose of this study was to detect genomic alterations in human endometrial cancer by two-dimensional gel electrophoresis.


METHODS
With use of a newly developed two-dimensional gel electrophoresis assay, we scanned 19 high-risk DNA fragments for alterations in human endometrial hyperplasias and adenocarcinomas. This method includes cleaving of high-molecular-weight DNA, radioactive labeling, and separating DNA fragments by two-dimensional gel electrophoresis. By comparing the two-dimensional gel electrophoresis profile (spots) of neoplastic with normal endometrium, genetic alterations such as amplification, allelic loss, and hypermethylation or hypomethylation can be detected.


RESULTS
Seven of 8 human endometrial adenocarcinoma (88%) and 1 of 2 hyperplasias (50%) revealed changes in spot density. The number of spots changed per specimen was 4. The median percentage of specimens with changes in an individual spot was 30%. Eleven spots had a reduction or loss of spot density, and 8 spots had an increase in spot density.


CONCLUSIONS
By use of a novel two-dimensional gel electrophoresis assay, we identified genetic alterations in 50% of hyperplasias and 88% of endometrial adenocarcinomas.
Pohlod-Miller
Susan
S
Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Medical College of Ohio, Toledo 43614-5809, USA.
Fanning
James
J
Gu
Ping
P
Crist
Keith A
KA
You
Ming
M
D000230:Adenocarcinoma / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004714:Endometrial Hyperplasia / Q000235:genetics; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D016678:Genome; D006801:Humans; D012016:Reference Values