Physiological study of pH stability and sensitivity to pepsin of human gastric lipase.
OBJECTIVE
The aim of this study was to exactly determine the pH stability of human gastric lipase (HGL) and to investigate the mechanism underlying the inactivation of HGL which occurs in gastric juice.


METHODS
Samples of human gastric juice and purified HGL were incubated at various pH values ranging from 0.5 to 8.0, and the residual HGL activity was measured as a function of time using the pHstat technique. Samples of purified HGL were also incubated in the presence of human pepsin. Electrophoresis and Western blot analysis were performed on all the samples in which HGL was inactivated.


RESULTS
HGL was found to be stable in gastric juice at pH values ranging from 2.0 to 7.0, especially between pH 3.0 and 5.0 (half-inactivation time >24 h). HGL activity decreased rapidly below pH 2.0 and above pH 7.0. The inactivation half times were only 43 +/- 9 and 24 +/- 18 min at pH 1 and pH 8, respectively. The pH stability of purified HGL was much lower than that of HGL in gastric juice. Acid or alkaline inactivation of HGL could occur without any prior proteolytic degradation, and this inactivation was irreversible. However, proteolytic degradation of HGL by pepsin also occurred at very low pH values, probably because the acid-denatured HGL is more sensitive to proteolytic cleavage by pepsin. An ex vivo study of HGL activity in several gastric juice samples showed that the HGL activity decreased with the pH of the sample, in both basal and pentagastrin-stimulated gastric juice.


CONCLUSIONS
Although HGL is not as stable as it was previously thought to be under acidic conditions, it is nevertheless the most stable acid lipase and constitutes a good candidate tool for enzyme substitution therapy.
Ville
Eric
E
La Timone Hospital, Gastroenterology Department, Marseille, France.
Carrière
Frédéric
F
Renou
Christophe
C
Laugier
René
R
D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005750:Gastric Juice / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000187:drug effects / Q000502:physiology*; D010434:Pepsin A / Q000494:pharmacology*; D013997:Time Factors
D008049:Lipase; D010434:Pepsin A