The functional significance of absence: the chromosomal segment harboring Tp53 is absent from the T55 rat radiation hybrid mapping panel.
The T55 rat radiation hybrid (RH) mapping panel has been reported to retain the entire rat genome at retention frequencies between 22% and 37%. However, we found that a small segment of rat chromosome 10 harboring at least four different genes, including Tp53, was completely absent from the panel (retention frequency = 0%). Two other markers located in the vicinity exhibited much reduced retention (2-6%). RH clones are generated by transferring highly fragmented DNA into a recipient cell. There might be a strong selection against the transfer and retention of chromosome segments harboring an intact Tp53, as the action of this gene might prevent proliferation and establishment of the RH clone. Our finding further suggests that unexpected low retention or absence of chromosome segments in an RH panel may represent indications that the segments harbor genes with important functions in cell proliferation control.
Behboudi
Afrouz
A
Department of Cell and Molecular Biology-Genetics, Lundberg Laboratory, Göteborg University, SE 405 30 Göteborg, Sweden. Afrouz.Behboudi@gen.gu.se
Roshani
Leyla
L
Lundin
Lisa
L
Ståhl
Fredrik
F
Levan
Karin Klinga
KK
Levan
Göran
G
D000818:Animals; D002874:Chromosome Mapping; D017353:Gene Deletion; D016158:Genes, p53; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D021101:Radiation Hybrid Mapping; D051381:Rats
D005819:Genetic Markers