title: Crystallization and preliminary X-ray crystallographic analysis of enoyl-acyl carrier protein reductase from Helicobacter pylori.
abstract: Enoyl-acyl carrier protein reductase (ENR) catalyzes the NADH-dependent stereospecific reduction of alpha,beta-unsaturated fatty acids bound to the acyl-carrier protein. ENR from Helicobacter pylori has been overexpressed in Escherichia coli and has been crystallized in the presence of its cofactor NADH and the inhibitor triclosan (or its analogue diclosan) at 296 K using polyethylene glycol (PEG) 400 as a precipitant. For the triclosan (or diclosan) complex, diffraction data to 2.5 (or 2.3) A resolution have been collected using synchrotron X-rays. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 73.35, b = 94.91, c = 75.38 A, beta = 106.21 degrees for the triclosan complex (or a = 73.25, b = 95.07, c = 75.02 A, beta = 106.53 degrees for the diclosan complex). The asymmetric unit contains one homotetramer, with a corresponding V(M) of 2.10 A(3) Da(-1) and a solvent content of 41% by volume.
authors.affiliation: Laboratory of Structural Proteomics, School of Chemistry and Molecular Engineering, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Hyung Ho
authors.initials: HH
authors.lastname: Lee
authors.forename: Jungmin
authors.initials: J
authors.lastname: Yun
authors.forename: Jinho
authors.initials: J
authors.lastname: Moon
authors.forename: Byung Woo
authors.initials: BW
authors.lastname: Han
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Lee
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Se Won
authors.initials: SW
authors.lastname: Suh
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D050753:Enoyl-(Acyl-Carrier-Protein) Reductase (NADH); D004791:Enzyme Inhibitors; D016480:Helicobacter pylori / Q000201:enzymology*; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000737:chemistry*; D011487:Protein Conformation; D014260:Triclosan / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D014260:Triclosan; D010088:Oxidoreductases; D050753:Enoyl-(Acyl-Carrier-Protein) Reductase (NADH)