title: Characterization of human metapneumoviruses isolated from patients in North America.
abstract: Human metapneumovirus (HMPV) was recently identified in The Netherlands and was linked to acute respiratory tract illness. In this study, 11 isolates from 10 patients with respiratory disease from Quebec, Canada, were tested by a reverse-transcriptase polymerase chain reaction based on the fusion protein gene. Identified sequences were consistent with HMPV. The patients were 2 months to 87 years of age (median age, 58 years) and presented with acute respiratory tract illness during the winter season. Sequence studies of the nucleocapsid, fusion, and polymerase genes identified 2 main lineages of HMPV and cocirculation of both lineages during the same year. These findings support a previous finding that HMPV is a human respiratory pathogen that merits further study.
authors.affiliation: Respiratory Virus Section, Respiratory and Enteric Viruses Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30333, USA.
authors.forename: Teresa C T
authors.initials: TC
authors.lastname: Peret
authors.forename: Guy
authors.initials: G
authors.lastname: Boivin
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Michel
authors.initials: M
authors.lastname: Couillard
authors.forename: Charles
authors.initials: C
authors.lastname: Humphrey
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: Dean D
authors.initials: DD
authors.lastname: Erdman
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Anderson
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D029121:Metapneumovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008875:Middle Aged; D008969:Molecular Sequence Data; D018184:Paramyxoviridae Infections / Q000821:virology*; D010802:Phylogeny; D011792:Quebec; D012141:Respiratory Tract Infections / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014760:Viral Fusion Proteins / Q000235:genetics
chemical_list: D014760:Viral Fusion Proteins