Oviedomycin, an unusual angucyclinone encoded by genes of the oleandomycin-producer Streptomyces antibioticus ATCC11891.
Our investigations on the discovery of novel natural metabolites using type II polyketide synthase gene probes (actI/III) yielded an unusual angucyclinone, oviedomycin (2), when applied to the oleandomycin (1) producer Streptomyces antibioticus ATCC11891. The novel natural product was produced using S. albus R(-)M(-) as a host strain, into which a cosmid containing the oviedomycin gene cluster was transformed. Its structure was elucidated by NMR spectroscopy and mass spectrometry.
Méndez
Carmen
C
Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, 33006 Oviedo, Spain.
Künzel
Eva
E
Lipata
Fredilyn
F
Lombó
Felipe
F
Cotham
William
W
Walla
Michael
M
Bearden
Daniel W
DW
Braña
Alfredo F
AF
Salas
Jose A
JA
Rohr
Jürgen
J
D000617:Aminoglycosides / Q000737:chemistry / Q000302:isolation & purification*; D004988:Ethers, Cyclic / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013303:Streptomyces antibioticus / Q000235:genetics* / Q000378:metabolism*
D000617:Aminoglycosides; D004988:Ethers, Cyclic; D009097:Multienzyme Complexes; C461780:oviedomycin