Mouse antibody response to a microsporidian parasite following inoculation with a gene coding for parasite ribosomal RNA.
This study found that a plasmid construct encoding the small-subunit ribosomal RNA (SSUrRNA) of the microsporidian Microgemma caulleryi generates a humoral response upon intramuscular inoculation in mice. The plasmid used was pCMV, following preliminary trials indicating efficient beta-galactosidase gene expression in mouse muscle cells transfected with pCMV/beta-Gal. The antibodies produced after inoculation with pCMV/SSUDNA recognized parasite spore antigens and reached maximum levels at 30 days postinoculation, subsequently remaining stable for at least 120 days. Due to the highly conserved sequence of the SSUrDNA in different microsporidian species, these results open up interesting prospects for broad-spectrum vaccination.
Leiro
J
J
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain. mpleiro@usc.es
Siso
M I G
MI
Iglesias
R
R
Ubeira
F M
FM
Sanmartín
M L
ML
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay; D017125:Genes, Protozoan; D005822:Genetic Vectors; D007273:Injections, Intramuscular; D007763:Lac Operon; D051379:Mice; D008807:Mice, Inbred BALB C; D016816:Microsporida / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D016052:Protozoan Vaccines / Q000008:administration & dosage*; D016053:RNA, Protozoan / Q000235:genetics / Q000276:immunology; D012335:RNA, Ribosomal / Q000235:genetics / Q000276:immunology*; D013170:Spores / Q000276:immunology; D014614:Vaccines, Synthetic
D000913:Antibodies, Protozoan; D016052:Protozoan Vaccines; D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D014614:Vaccines, Synthetic