Constituents of Chinese propolis and their antiproliferative activities.
Two new flavonoids, 3-O-[(S)-2-methylbutyroyl]pinobanksin (1) and 6-cinnamylchrysin (2), were isolated from the EtOAc-soluble fraction of the MeOH extract of Chinese propolis, along with 12 known compounds (3-14). The structures of the isolated compounds were elucidated on the basis of spectroscopic and chemical analyses. The isolated compounds were tested for their antiproliferative activity toward five different cancer cell lines. Benzyl caffeate (13) and phenethyl caffeate (14) showed potent antiproliferative activity toward tested cell lines with a selective activity toward colon 26-L5 carcinoma cell line (EC(50) values: 13, 1.01; 14, 0.30 microM).
Usia
Tepy
T
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630-Sugitani, Toyama 930-0194, Japan.
Banskota
Arjun H
AH
Tezuka
Yasuhiro
Y
Midorikawa
Kiyoshi
K
Matsushige
Katsumichi
K
Kadota
Shigetoshi
S
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001713:Biphenyl Compounds; D002109:Caffeic Acids / Q000737:chemistry / Q000494:pharmacology; D002681:China; D002855:Chromatography, Thin Layer; D003110:Colonic Neoplasms; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D015394:Molecular Structure; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D011429:Propolis / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D014407:Tumor Cells, Cultured / Q000187:drug effects
C458536:3-O-(2-methylbutyroyl)pinobanksin; C458537:6-cinnamylchrysin; D000970:Antineoplastic Agents; D001713:Biphenyl Compounds; D002109:Caffeic Acids; D005419:Flavonoids; D016166:Free Radical Scavengers; D010851:Picrates; C115232:benzyl caffeate; D011429:Propolis; C004931:1,1-diphenyl-2-picrylhydrazyl; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol