Pharmacological effects of Eugenia uniflora (Myrtaceae) aqueous crude extract on rat's heart.
The effect of aqueous crude extract (ACE) of Eugenia uniflora L. (Myrtaceae) was studied on rat's perfused ventricles. This plant is used in South American traditional medicine as an antihypertensive and we already demonstrated previously its hypotensive properties. In this paper, maximal left intraventriclular pressure (P) of rat's hearts beating at 0.2 Hz firstly increased to 162.1+/-11.1% of basal value during 1-3 min of perfusing ACE 0.6%. Maximum rate of contraction (+P) also increased to duplicating +P/P ratio. Both types of effect were significantly decreased by either propranolol 0.35 microM, and pre-treatment with reserpine (5 mg/kg), suggesting that they were caused by a compound that releases cathecolamines with beta-adrenergic action. Nevertheless, after 20 min of perfusing ACE, ventricles decreased P to about 50% of their basal value, suggesting a negative-inotropic compound present in the extract. The perfusion of 1.2% ACE decreased P in a pressure-[Ca](o) curve (0.5-2 mM) in a non-competitive manner, suggesting that an irreversible Ca-blocking compound is also present in the extract. In summary, E. uniflora ACE has a dual effect on the heart related to its hypotensive action and is probably responsible for the therapeutic or adverse effects in patients under cardiac risk.
Consolini
Alicia E
AE
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Cátedra de Farmacodinamia, Universidad Nacional de La Plata, 47 y 115 (1900), La Plata, Argentina. dinamia@nahuel.biol.unlp.edu.ar
Sarubbio
Marisol Gracía
MG
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004347:Drug Interactions; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D006352:Heart Ventricles / Q000187:drug effects*; D008297:Male; D008519:Medicine, Traditional; D009200:Myocardial Contraction / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012110:Reserpine / Q000494:pharmacology; D027842:Syzygium; D013997:Time Factors
D010936:Plant Extracts; D012110:Reserpine; D011433:Propranolol