Novel bacteriochlorophyll e structures and species-specific variability of pigment composition in green sulfur bacteria.
The relative composition of bacteriochlorophyll (BChl) homologs in five different strains of brown-colored green sulfur bacteria was investigated by HPLC-MS/MS and NMR analyses. In addition, the effect of incubation light intensities on homolog distribution was studied in one of the strains (strain Dagow III). A total of 23 different BChl e structures were detected and comprise four homologous porphyrin ring systems and eight different esterifying alcohols. Several BChl e structures are novel. These include a C-8 ethyl, C-12 methyl [E, M] BChl e(F) homolog which was identified by (1)H-NMR analyses of the isolated, main farnesyl homologs (BChl e(F)). In addition, five previously unknown homolog series with dodecanol, pentadecenol, tetradecanol, hexadecenol and phytol as the esterifying alcohols were detected. The composition of BChl e homologs from the five strains of green sulfur bacteria differed with respect to the relative abundance of the homologs (BChl e(F) : 25.6-67.0% of total BChl e content in stationary cultures). In strain Dagow III, the abundance of BChl e(F) homologs decreased upon entry into the stationary phase. In all free-living strains, the abundance of BChl e(F) was increased when the relative carotenoid content was low. The present results provide a detailed picture of pigment composition in chlorosomes and thus will help to elucidate their structure and function. Furthermore, the newly discovered BChl e molecules are valuable biomarkers for the study of the occurrence and metabolism of green sulfur bacteria in past and present ecosystems.
Glaeser
Jens
J
Ludwig-Maximilians-Universität München, Institut für Genetik und Mikrobiologie, Maria-Ward-Strasse 1a, 80638 Munich, Germany.
Bañeras
Lluis
L
Rütters
Heike
H
Overmann
Jörg
J
D001429:Bacteriochlorophylls / Q000032:analysis / Q000737:chemistry*; D002338:Carotenoids / Q000032:analysis / Q000145:classification; D019414:Chlorobi / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013045:Species Specificity
D001429:Bacteriochlorophylls; C120930:bacteriochlorophyll e; D002338:Carotenoids