title: Identification and quantification of galloyl derivatives, flavonoid glycosides and anthocyanins in leaves of Pistacia lentiscus L.
abstract: Separation, identification and quantification of polyphenols was carried out on leaves of Pistacia lentiscus L., an evergreen member of the family Anacardiaceae, using semi-preparative HPLC, HPLC-photodiode array detection and HPLC-MS analysis, together with 1H- and 13C NMR. Three major classes of secondary metabolites were detected: (i) gallic acid and galloyl derivatives of both glucose and quinic acid; (ii) flavonol glycosides, i.e. myricetin and quercetin glycosides; and (iii) anthocyanins, namely delphinidin 3-O-glucoside and cyanidin 3-O-glucoside. Low amounts of catechin were also detected. The concentration of galloyl derivatives was extremely high, representing 5.3% of the leaf dry weight, and appreciable amounts of myricetin derivatives were also detected (1.5% on a dry weight basis). These findings may be useful in establishing a relationship between the chemical composition of the leaf extract and the previously reported biological activity of P. lentiscus, and may also assign a new potential role of P. lentiscus tissue extracts in human health care.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università degli Studi, Via G. Capponi 9, I-50121, Firenze, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Romani
authors.forename: P
authors.initials: P
authors.lastname: Pinelli
authors.forename: C
authors.initials: C
authors.lastname: Galardi
authors.forename: N
authors.initials: N
authors.lastname: Mulinacci
authors.forename: M
authors.initials: M
authors.lastname: Tattini
mesh_terms: D000872:Anthocyanins / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D027927:Pistacia / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D011108:Polymers / Q000032:analysis* / Q000737:chemistry
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D006027:Glycosides; D010636:Phenols; D011108:Polymers; D005707:Gallic Acid