A novel regulatory element determines the timing of Mos mRNA translation during Xenopus oocyte maturation.
Progression through vertebrate oocyte maturation requires that pre-existing, maternally derived mRNAs be translated in a strict temporal order. The mechanism that controls the timing of oocyte mRNA translation is unknown. In this study we show that the early translational induction of the mRNA encoding the Mos proto-oncogene is mediated through a novel regulatory element within the 3' untranslated region of the Mos mRNA. This novel element is responsive to the MAP kinase signaling pathway and is distinct from the late acting, cdc2-responsive, cytoplasmic polyadenylation element. Our findings suggest that the timing of maternal mRNA translation is controlled through signal transduction pathways targeting distinct 3' UTR mRNA elements.
Charlesworth
Amanda
A
Department of Anatomy and Neurobiology, University of Arkansas for Medical Sciences, 4301 West Markham Street Slot 814, Little Rock, AR 72205, USA.
Ridge
John A
JA
King
Leslie A
LA
MacNicol
Melanie C
MC
MacNicol
Angus M
AM
D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D016203:CDC2 Protein Kinase / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D020935:MAP Kinase Signaling System; D008954:Models, Biological; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009865:Oocytes / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D026723:Polyadenylation; D011374:Progesterone / Q000378:metabolism; D014176:Protein Biosynthesis; D017231:Proto-Oncogene Proteins c-mos / Q000378:metabolism* / Q000502:physiology; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013997:Time Factors; D014981:Xenopus / Q000378:metabolism*
D020413:3' Untranslated Regions; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D011374:Progesterone; D012313:RNA; D005982:Glutathione Transferase; D017231:Proto-Oncogene Proteins c-mos; D016203:CDC2 Protein Kinase