title: Rational use of the PFA-100 device for screening of platelet function disorders and von Willebrand disease.
abstract: Two hundred and five patients referred for evaluation of platelet functions and 126 healthy controls were tested with the PFA-100 instrument. A cut-off value of 150 s for collagen/epinephrine (CEPI) closure time (CT) produced most acceptable sensitivity (90%), specificity (85.2%), and positive (82.6%) and negative (91.6%) predictivity values for screening of platelet function disorders and von Willebrand disease (vWD). All patients with vWD and Glanzmann thrombasthenia could be detected by PFA-100. Both CEPI and collagen/adenosine diphosphate (CADP) CTs were elevated in all of these cases. Sensitivity of the device was 81.6% for patients with platelet secretion defects. CADP CT was normal in 63.9% of the patients in this subgroup. Specificity (47%) and positive predictivity (57%) of the instrument were diminished in patients with low hemoglobin concentrations. Depending on the results, an algorithm was developed for screening of platelet function disorders and vWD with PFA-100.
authors.affiliation: Department of Hematology, Hacettepe University Medical School, Ankara, Turkey. ybuyukas@hacettepe.edu.tr
authors.forename: Y
authors.initials: Y
authors.lastname: Buyukasik
authors.forename: S
authors.initials: S
authors.lastname: Karakus
authors.forename: H
authors.initials: H
authors.lastname: Goker
authors.forename: I C
authors.initials: IC
authors.lastname: Haznedaroglu
authors.forename: D
authors.initials: D
authors.lastname: Ozatli
authors.forename: N
authors.initials: N
authors.lastname: Sayinalp
authors.forename: O I
authors.initials: OI
authors.lastname: Ozcebe
authors.forename: S V
authors.initials: SV
authors.lastname: Dundar
authors.forename: S
authors.initials: S
authors.lastname: Kirazli
mesh_terms: D000465:Algorithms; D016022:Case-Control Studies; D003663:Decision Trees; D019719:Diagnostic Equipment; D006801:Humans; D010979:Platelet Function Tests / Q000295:instrumentation / Q000379:methods / Q000592:standards; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013915:Thrombasthenia / Q000097:blood / Q000175:diagnosis; D014842:von Willebrand Diseases / Q000097:blood / Q000175:diagnosis*