Mechanisms of induction of germination of Bacillus subtilis spores by high pressure.
Spores of Bacillus subtilis lacking all germinant receptors germinate >500-fold slower than wild-type spores in nutrients and were not induced to germinate by a pressure of 100 MPa. However, a pressure of 550 MPa induced germination of spores lacking all germinant receptors as well as of receptorless spores lacking either of the two lytic enzymes essential for cortex hydrolysis during germination. Complete germination of spores either lacking both cortex-lytic enzymes or with a cortex not attacked by these enzymes was not induced by a pressure of 550 MPa, but treatment of these mutant spores with this pressure caused the release of dipicolinic acid. These data suggest the following conclusions: (i) a pressure of 100 MPa induces spore germination by activating the germinant receptors; and (ii) a pressure of 550 MPa opens channels for release of dipicolinic acid from the spore core, which leads to the later steps in spore germination.
Paidhungat
Madan
M
Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA.
Setlow
Barbara
B
Daniels
William B
WB
Hoover
Dallas
D
Papafragkou
Efstathia
E
Setlow
Peter
P
D001412:Bacillus subtilis / Q000502:physiology*; D010848:Picolinic Acids / Q000378:metabolism; D011312:Pressure; D013171:Spores, Bacterial / Q000378:metabolism / Q000502:physiology*
D010848:Picolinic Acids; C004860:dipicolinic acid