Full-length complementary DNA and the derived amino acid sequence of horse uteroglobin.
After its original description as a steroid-dependent protein in the rabbit uterus, uteroglobin became one of the best characterized proteins. However, detailed knowledge of its physiological role remains an enigma. In this study we investigate how its structure is phylogenetically conserved in the horse compared to other mammalian species. Northern blot analysis showed that in horses, the main expression of uteroglobin appears in lung, uterus, and prostate tissues. Western blot analysis demonstrated that the dimeric form of uteroglobin is found predominantly in biological compartments. Using a RACE-PCR technique, we cloned and sequenced the full-length cDNA (473 base pairs) that encodes equine uteroglobin. The nucleotide sequence was shown to characterize the primary structure of this protein. This enabled us to add equine uteroglobin to a comparative amino acid alignment of 8 other uteroglobin molecules, and finally, to unravel 14 evolutionary completely conserved amino acids. We summarize these results with a computer-based 3-D model of horse uteroglobin, and discuss new concepts on the physiological role of uteroglobin, in particular as a specific binding protein.
Müller-Schöttle
Frank
F
Department of Anatomy and Reproductive Biology, Medical School, RWTH University of Aachen, D-52057 Aachen, Germany.
Bogusz
Agata
A
Grötzinger
Joachim
J
Herrler
Andreas
A
Krusche
Claudia A
CA
Beier-Hellwig
Karin
K
Beier
Henning M
HM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D003198:Computer Simulation; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry*; D019143:Evolution, Molecular; D006736:Horses; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014598:Uteroglobin / Q000737:chemistry* / Q000235:genetics*
D018076:DNA, Complementary; D014598:Uteroglobin