title: Interleukin-1beta activity and collagen synthesis in human dental pulp fibroblasts.
abstract: Immunopathologic reactions play a significant role in inflammatory diseases of dental pulp. Interleukin-1beta (IL-1beta) is recognized as a key player in mediating cellular immune response. In this study, we measured the content of IL-1beta and its effect on collagen synthesis in cultures of fibroblasts derived from healthy and diseased dental pulps. We found that diseased pulp fibroblasts contain 2.5-fold greater amounts of IL-1beta and synthesized 80% greater amounts of collagen compared with healthy pulp fibroblasts. However, exogenous IL-1beta failed to stimulate collagen synthesis by diseased fibroblasts, whereas collagen synthesis by healthy pulp fibroblasts was stimulated by more than 2-fold. These observations imply that pulp disease induces abnormalities associated with fibroblast response toward IL-1beta.
authors.affiliation: Department of Preventive & Restorative Dental Sciences, School of Dentistry, University of California, San Francisco 94143, USA.
authors.forename: Rahmat A
authors.initials: RA
authors.lastname: Barkhordar
authors.forename: Q Perveen
authors.initials: QP
authors.lastname: Ghani
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Russell
authors.forename: M Zamirul
authors.initials: MZ
authors.lastname: Hussain
mesh_terms: D000704:Analysis of Variance; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000494:pharmacology / Q000502:physiology*; D011671:Pulpitis / Q000378:metabolism*
chemical_list: D024042:Collagen Type I; D007375:Interleukin-1