Target-assisted iterative screening reveals novel interactors for PSD95, Nedd4, Src, Abl and Crk proteins.
A new in vitro screening method has been developed and applied to a commercial phage-displayed cDNA library to search for novel protein-protein interactions. PDZ, WW and SH3 domains from PSD95, Nedd4, Src, Abl and Crk proteins were used as targets. 12 novel putative and 2 previously reported interactions were identified in test screens. The novel screening format, dubbed TAIS (target-assisted iterative screening), is discussed as an alternative platform to existing technologies for a pair-wise characterization of protein-protein interactions.
Kurakin
Alexei
A
Buck Institute for Age Research, 8001 Redwood Blvd., Novato, CA 94945, USA. akourakine@buckinstitute.org
Bredesen
Dale
D
D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D020740:Chimerin Proteins / Q000378:metabolism; D000076122:Disks Large Homolog 4 Protein; D056827:Endosomal Sorting Complexes Required for Transport; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008025:Ligases / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000378:metabolism; D062551:NAV1.2 Voltage-Gated Sodium Channel; D000075702:Nedd4 Ubiquitin Protein Ligases; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D000070798:Nogo Proteins; D009842:Oligopeptides / Q000378:metabolism*; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism*; D050737:Proto-Oncogene Proteins c-crk; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017385:Sequence Homology; D015222:Sodium Channels / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases
D002135:Calcium-Binding Proteins; D020740:Chimerin Proteins; C501444:DLG4 protein, human; D000076122:Disks Large Homolog 4 Protein; D056827:Endosomal Sorting Complexes Required for Transport; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009185:Myelin Proteins; D062551:NAV1.2 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins; D000070798:Nogo Proteins; D009842:Oligopeptides; D011518:Proto-Oncogene Proteins; D050737:Proto-Oncogene Proteins c-crk; C000603199:RTN4 protein, human; D011993:Recombinant Fusion Proteins; C568251:SCN2A protein, human; D015222:Sodium Channels; C040475:Src peptide; C033652:postsynaptic density proteins; C100848:LAPTM5 protein, human; D000075702:Nedd4 Ubiquitin Protein Ligases; C000618483:Nedd4 protein, human; D044767:Ubiquitin-Protein Ligases; D016315:Proto-Oncogene Proteins c-abl; D008025:Ligases