title: The determination of protein phosphorylation on electrophoresis gel blots by laser ablation inductively coupled plasma-mass spectrometry.
abstract: Laser ablation interfaced with inductively coupled plasma-mass spectrometry is described as a new method to determine the presence of phosphorylated proteins on electrophoresis gel blots. The method was applied to the phosphoprotein beta-casein with good detection levels being observed at 16 pmole. Attempts at using the technique to detect beta-casein on electrophoresis gels are also described.
authors.affiliation: Computational and Structural Sciences, GlaxoSmithKline, Stevenage, UK. PSM0667@gsk.com
authors.forename: Peter
authors.initials: P
authors.lastname: Marshall
authors.forename: Olivier
authors.initials: O
authors.lastname: Heudi
authors.forename: Satty
authors.initials: S
authors.lastname: Bains
authors.forename: Hamzah Neil
authors.initials: HN
authors.lastname: Freeman
authors.forename: Fadi
authors.initials: F
authors.lastname: Abou-Shakra
authors.forename: Kevin
authors.initials: K
authors.lastname: Reardon
mesh_terms: D002364:Caseins / Q000032:analysis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007834:Lasers; D010766:Phosphorylation
chemical_list: D002364:Caseins