Novel effects of diets enriched with corn oil or with an olive oil/sunflower oil mixture on vitamin K metabolism and vitamin K-dependent proteins in young men.
Little is known of how the fat components of diets influence the absorption and metabolism of vitamin K and the possible consequences to the synthesis of vitamin K-dependent (VKD) proteins in different target organs. We have evaluated the effects of two diets on circulating phylloquinone (K1) and triacylglycerols (TAG). One diet was enriched with corn oil (CO) (also rich in gamma-tocopherol) and the other with an olive/sunflower (O/SO) mixture (rich in alpha-tocopherol). Effects on gamma-carboxylation were assessed from coagulation assays and sensitive assays for undercarboxylated prothrombin (ucFII) and osteocalcin (ucOC). Total plasma matrix Gla-protein (MGP) was also measured. After an initial adjustment diet, 26 healthy young men were fed, in a crossover design, the O/SO or CO diet for 2 weeks. Mean intakes of K1 during consumption of adjustment, O/SO, and CO diets were 225 microg/day, 291 microg/day, and 291 microg/day, respectively. Mean fasting levels of TAG and K1 were both significantly reduced by the CO diet, but not by the O/SO diet. Neither diet reduced FII activity but ucFII became detectable in nine subjects, eight of whom showed this abnormality with both diets. The CO diet induced a rise in ucOC (P < 0.05), which was negatively correlated to ucFII (r = -0.71, P < 0.03). The CO but not O/SO diet induced a decrease of total circulating MGP. We conclude that both oils, notably CO, affected vitamin K absorption and/or metabolism which may increase the requirements for gamma-carboxylation. The mechanism is unclear but may result from interactions of vitamin K with PUFA and/or other lipid components such as vitamin E.
Schurgers
Leon J
LJ
Department of Biochemistry and Cardiovascular Research Institute, Maastricht University, The Netherlands.
Shearer
Martin J
MJ
Soute
Berry A M
BA
Elmadfa
Ibrahim
I
Harvey
Julia
J
Wagner
Karl-Heinz
KH
Tomasch
Richard
R
Vermeer
Cees
C
D000328:Adult; D003314:Corn Oil / Q000494:pharmacology*; D006801:Humans; D008297:Male; D000069463:Olive Oil; D010938:Plant Oils / Q000494:pharmacology*; D000074242:Sunflower Oil; D014280:Triglycerides / Q000097:blood; D014812:Vitamin K / Q000097:blood / Q000378:metabolism*
D000069463:Olive Oil; D010938:Plant Oils; D000074242:Sunflower Oil; D014280:Triglycerides; D014812:Vitamin K; D003314:Corn Oil