LC-ion trap electrospray MS-MS for the determination of cyclopiazonic acid in milk samples.
An LC-ESI-MS (negative ions) method was developed for the determination of cyclopiazonic acid (CPA), a mycotoxin produced by many Aspergillus and Penicillium genuses, in milk samples. First, the acquisition parameters of the ESI mass spectrometer were optimised for deprotonated CPA and MS-MS measurements were performed, giving a fragmentation pattern. After this stage, LC separation was applied to milk extracts (with or without CPA spikes) by means of an aminic column, using the selective reaction monitoring (SRM) acquisition mode for MS detection. The CPA response was linear over three decades of concentration and an LOD of 5 ng mL(-1) was estimated; moreover, the extraction procedure produced almost quantitative recoveries of CPA from milk. Twenty different milk samples were analysed and three of them were found to be contaminated with CPA to various extents.
Losito
Ilario
I
Dipartimento di Chimica, Università degli Studi di Bari, Italy. losito@chimica.uniba.it
Monaci
Linda
L
Aresta
Antonella
A
Zambonin
Carlo G
CG
D000818:Animals; D005506:Food Contamination / Q000032:analysis*; D007211:Indoles / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D007211:Indoles; C000543:cyclopiazonic acid