Clinical study of a urinary competitve ELISA for neural thread protein in Alzheimer disease.
AD7C-NTP (neural thread protein) is a approximately 41-kD brain protein that is selectively elevated in Alzheimer disease (AD). AD7C-NTP is associated with the pathologic changes of AD, and overexpression of the AD7C-NTP gene is associated with cell death similar to that found in the AD brain. A newly developed competitive ELISA (enzyme-linked immunosorbent assay) was tested in urine samples from patients with AD, patients with non-AD dementia, and healthy normal individuals. Mean assay measurement in the AD group (30.1 +/- 10.8) was significantly higher than in the non-AD dementia control group (13.4 +/- 3.4) and in the nondementia control group (14.8 +/- 5.2) (P <.001). Mean assay measurement in early-AD cases (25.3 +/- 7.6) was significantly lower than in other AD cases (33.9 +/- 11.4). Levels of more than 18 units were found in 89% of overall AD cases and in 10% of overall controls. The results further validate urinary AD7C-NTP as a biochemical marker for AD and indicate that the competitive ELISA-format AD7C-NTP test in urine is an accurate method for determining AD7C-NTP levels in AD.
Munzar
Michael
M
Nymox Pharmaceutical Corporation, Montreal, Canada. info@nyrnox.com
Levy
Suzanna
S
Rush
Robert
R
Averback
Paul
P
D000328:Adult; D000368:Aged; D000544:Alzheimer Disease / Q000175:diagnosis* / Q000652:urine*; D001667:Binding, Competitive; D015415:Biomarkers / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000652:urine*; D009422:Nervous System Diseases / Q000652:urine; D011237:Predictive Value of Tests; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
C109804:AD7c-NTP; D015415:Biomarkers; D009419:Nerve Tissue Proteins