Morgue mediates apoptosis in the Drosophila melanogaster retina by promoting degradation of DIAP1.
Inhibitor of apoptosis proteins (IAPs) provide a critical barrier to inappropriate apoptotic cell death through direct binding and inhibition of caspases. We demonstrate that degradation of IAPs is an important mechanism for the initiation of apoptosis in vivo. Drosophila Morgue, a ubiquitin conjugase-related protein, promotes DIAP1 down-regulation in the developing retina to permit selective programmed cell death. Morgue complexes with DIAP1 in vitro and mediates DIAP1 degradation in a manner dependent on the Morgue UBC domain. Reaper (Rpr) and Grim, but not Hid, also promote the degradation of DIAP1 in vivo, suggesting that these proteins promote cell death through different mechanisms.
Hays
Rebecca
R
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, 660 South Euclid Avenue; Campus Box 8103, Saint Louis, MO 63110, USA.
Wickline
Laura
L
Cagan
Ross
R
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002875:Chromosomes; D017124:Conserved Sequence; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D066298:In Vitro Techniques; D051034:Inhibitor of Apoptosis Proteins; D008025:Ligases / Q000235:genetics; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D017956:Photoreceptor Cells, Invertebrate / Q000166:cytology / Q000196:embryology / Q000201:enzymology; D012160:Retina / Q000166:cytology* / Q000196:embryology / Q000201:enzymology; D025801:Ubiquitin / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes
C111891:DIAP1 protein, Drosophila; D029721:Drosophila Proteins; D005136:Eye Proteins; C108880:HID protein, Drosophila; D051034:Inhibitor of Apoptosis Proteins; D009479:Neuropeptides; D010455:Peptides; D025801:Ubiquitin; C108881:grim protein, Drosophila; C460739:morgue protein, Drosophila; C086973:rpr protein, Drosophila; D044763:Ubiquitin-Conjugating Enzymes; D008025:Ligases