[Protective effects of d-, l-, and dl-3-n-butylphthalide on neuronal damage induced by hypoxia/hypoglycemia in cultured rat cortical neurons].
The effects of l-3-n-butylphthalide(l-NBP) and d-3-n-butylphthalide(d-NBP) on hypoxia/hypoglycemia-induced cytotoxicity in primary cultured rat cortical neurons were studied. l-NBP and d-NBP(1-100 mumol.L-1) were shown to inhibit hypoxia/hypoglycemia-induced LDH release, decrease the percent of cell death and improve the damaged cellular morphology at 10 mumol.L-1 concentration. In addition, l-NBP, d-NBP and dl-NBP were also found to significantly reduce the liberation of polyribosomes from the neuronal rough endoplasmic reticulum and disaggregation of polyribosomes induced by hypoxia/hypoglycemia. These data suggest that l-NBP, d-NBP and dl-NBP can remarkably protect cultured neurons against hypoxia/hypoglycemia induced damage.
Yan
C
C
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050.
Feng
Y
Y
D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005333:Fetus; D007003:Hypoglycemia; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011132:Polyribosomes / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism
D001572:Benzofurans; D018696:Neuroprotective Agents; C027125:3-n-butylphthalide; D007770:L-Lactate Dehydrogenase