Photochemical control of the infectivity of adenoviral vectors using a novel photocleavable biotinylation reagent.
We have explored a novel strategy for controlling the infectivity of adenoviral vectors. This strategy involves a method whereby the infectivity of adenoviral vectors is neutralized by treatment of viral particles with a water-soluble, photocleavable biotinylation reagent. These modified viral vectors possess little to no infectivity for target cells. Exposure of these modified viral vectors to 365 nm light induces a reversal of the neutralizing, chemical modification, resulting in restoration of infectivity to the viral vectors. The light-directed transduction of target cells by photoactivatable adenoviral vectors was demonstrated successfully both in vitro and in vivo. This photochemical infectivity trigger possesses great potential, both as a research tool and as a novel tactic for the delivery of gene-transfer agents, since the infectivity of adenoviral vectors can be controlled externally in a versatile manner.
Pandori
Mark W
MW
Center for Molecular Imaging Diagnosis and Therapy and Basic Science Laboratory, Department of Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
Hobson
David A
DA
Olejnik
Jerzy
J
Krzymanska-Olejnik
Edyta
E
Rothschild
Kenneth J
KJ
Palmer
Abraham A
AA
Phillips
Tamara J
TJ
Sano
Takeshi
T
D000256:Adenoviridae / Q000187:drug effects / Q000472:pathogenicity* / Q000528:radiation effects; D000818:Animals; D001710:Biotin / Q000138:chemical synthesis / Q000737:chemistry*; D019921:Biotinylation / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D002470:Cell Survival / Q000528:radiation effects; D004285:Dogs; D015870:Gene Expression / Q000235:genetics; D005822:Genetic Vectors / Q000187:drug effects / Q000528:radiation effects*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D010777:Photochemistry / Q000379:methods; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D014774:Virulence / Q000528:radiation effects*
D001710:Biotin