[Studies on the anticancer effect of howiinol A, a new compound isolated from Goniothalamus howii].
The effect of howiinol A (GHM-10) on the growth of several cancer and normal cells were studied, using the methods of cell growth curve determination, MTT test and soft-agar colony formation assay. The results showed that GHM-10 exhibited potent inhibitory effect on cancer cells with an IC50 of 2 micrograms.ml-1 approximately. Normal cells, especially bone marrow progenitor cells, were shown to be less sensitive to GHM-10. A drug-resistant cell line, KB/VCR200, was found to be as sensitive as its parental KB cells. GHM-10 did not induce HL-60 cell differentiation. In murine transplantable tumors, GHM-10 was found to have significant therapeutic effect on mice bearing solid tumor hepatoma H22, Lewis lung carcinoma and S180 ascites.
He
J
J
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050.
Ye
Y
Y
Xu
C
C
D000818:Animals; D029061:Annonaceae / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000627:therapeutic use*; D018827:Carcinoma, Lewis Lung / Q000188:drug therapy; D005260:Female; D006801:Humans; D007624:KB Cells; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D024301:Plant Bark / Q000737:chemistry; D011753:Pyrones / Q000302:isolation & purification / Q000627:therapeutic use*; D012510:Sarcoma 180 / Q000188:drug therapy
D000972:Antineoplastic Agents, Phytogenic; D011753:Pyrones; C426486:howiinol A