title: 4-chlorophenol degradation by chloroperoxidase from Caldariomyces fumago: formation of insoluble products.
abstract: This study investigated the degradation of 4-chlorophenol (4-CP) by Caldariomyces fumago chloroperoxidase (CPO). Enzymatic oxidations were studied in reaction mixtures at pH 3.0, 4.0, and 6.0 in the presence and absence of Cl- containing 3.5 IU of CPO and 4-CP and hydrogen peroxide concentrations within the range of 0.5-50 and 0.005-50 mM, respectively. Distinct patterns of products regarding color, concentration, and solubility were observed. Reaction mixtures at pH 6.0 containing 3.5 IU of CPO and 5.0 mM 4-CP and H2O2 (1:1 stoichiometry) showed the highest 4-CP removal of 95% and the highest formation of a dark precipitate.
authors.affiliation: Laboratório de Tecnologia Enzimática, Instituto de Química, Universidade Federal do Rio de Janeiro, RJ, Brasil.
authors.forename: Camilo E
authors.initials: CE
authors.lastname: La Rotta
authors.forename: Elba P S
authors.initials: EP
authors.lastname: Bon
mesh_terms: D001105:Archaea / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002711:Chloride Peroxidase / Q000378:metabolism; D002733:Chlorophenols / Q000493:pharmacokinetics*; D005285:Fermentation; D006861:Hydrogen Peroxide / Q000378:metabolism; D007700:Kinetics
chemical_list: D002733:Chlorophenols; C029107:4-chlorophenol; D006861:Hydrogen Peroxide; D002711:Chloride Peroxidase