title: Cloning of mouse genomic ribosomal protein L6 gene and analysis of its promoter.
abstract: Ribosomal protein (Rp) L6 is also defined as Taxreb107 (Tax responsive element binding protein 107) for its binding activity to the long terminal repeats of human T cell leukemia virus (HTLV)-I. We cloned the genomic gene of mouse RpL6/Taxreb107 and analyzed its exon/intron structures. The promoter of RpL6/Taxreb107 contains recognition sites for multiple transcription factors including nuclear factor (NF)-kappa B. Luciferase reporter assay showed that the RpL6/Taxreb107 promoter has a constitutive activity in transfected cells, and the constitutive activity depends on the intact promoter. Expression of HTLV-I viral protein Tax mildly but reproducibly induced RpL6/Taxreb107 mRNA and promoter activity. We provide evidence suggesting that induction of RpL6/Taxreb107 by Tax is at least partially mediated by the NF-kappa B site in the promoter of RpL6/Taxreb107. Taken together, Tax up-regulates RpL6/Taxreb107 and this may provide a feedback mechanism to facilitate proliferation of HTLV-I-infected cells and production of viral particles.
authors.affiliation: Department of Medical Genetics and Developmental Biology, The Fourth Military Medical University, Xian 710032, People's Republic of China.
authors.forename: Jishu
authors.initials: J
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Hua
authors.initials: H
authors.lastname: Han
mesh_terms: D000818:Animals; D019556:COS Cells; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D016356:Gene Products, tax / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000737:chemistry / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D016356:Gene Products, tax; D016328:NF-kappa B; D012269:Ribosomal Proteins; C031249:ribosomal protein L6; C093544:tax-responsive-element-binding protein 107, mouse