title: [Construction and expression of a vector containing protein transduction domain and bcr/abl fusion gene].
abstract: OBJECTIVE
  To construct a vector containing protein transduction domain (PTD) and bcr/abl fusion gene of chronic myelogenous leukemia and express PTD-bcr/abl fusion protein in E. Coli.
  
  
  METHODS
  DNA fragment encoding PTD was synthesized and fused to PCR-amplified bcr/abl gene fragment, then inserted into plasmid pET-16b to get the expression vector pEPb containing PTD-bcr/abl fusion gene, which was transfected and expressed in E. Coli LB21. PTD-bcr/abl fusion protein was purified by affinity chromatography.
  
  
  RESULTS
  523 bp bcr/abl fusion gene was effectively amplified. The PTD-bcr/abl gene sequencing showed the same sequence as scheduled. The fusion peptide was successfully expressed in E. Coli and purified.
  
  
  CONCLUSIONS
  The results may provide a new PTD-bcr/abl fusion peptide for the immunotherapy of CML.
authors.affiliation: Department of Hematology of Tangdu Hospital and Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Liang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.forename: Shanshan
authors.initials: S
authors.lastname: Jiang
authors.forename: Jizhu
authors.initials: J
authors.lastname: Wang
authors.forename: Rongli
authors.initials: R
authors.lastname: Wu
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Han
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D016044:Fusion Proteins, bcr-abl / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D015696:Gene Products, tat / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D016044:Fusion Proteins, bcr-abl