title: Actinomyces species, streptococci, and Enterococcus faecalis in primary root canal infections.
abstract: The purpose of this study was to evaluate the prevalence of Actinomyces species, streptococci, and Enterococcus faecalis in primary root canal infections by using a molecular genetic method. Samples were obtained from 53 infected teeth, of which 27 cases were diagnosed as acute periradicular abscesses. DNA was extracted to evaluate the occurrence of 13 bacterial species by using whole genomic DNA probes and checkerboard DNA-DNA hybridization. Polymerase chain reaction using an ubiquitous bacterial primer was undertaken to check the presence of bacterial DNA in clinical samples. All root canal samples contained bacteria as demonstrated by polymerase chain reaction. The checkerboard DNA-DNA hybridization assay allowed the detection of streptococci in 22.6% of the samples, Actinomyces species in 9.4%, and E. faecalis in 7.5%. The most prevalent species were members of the Streptococcus anginosus group. With regard to the asymptomatic lesions, the most prevalent species were S. intermedius (11.5% of the cases), E. faecalis (11.5%), and S. anginosus (7.7%). S. constellatus was the most prevalent species in pus samples (25.9% of the cases). The other most prevalent species in abscessed teeth were A. gerencseriae (14.8%), S. gordonii (11.1%), S. intermedius (11.1%), A. israelii (7.4%), S. anginosus (7.4%), and S. sanguis (7.4%). S. constellatus was the only species positively associated with acute periradicular abscess (p < 0.01).
authors.affiliation: Oral Microbiology Laboratory, Institute of Microbiology, Federal University of Rio de Janeiro, RJ, Brazil.
authors.forename: José F
authors.initials: JF
authors.lastname: Siqueira
authors.forename: Isabela N
authors.initials: IN
authors.lastname: Rôças
authors.forename: Renata
authors.initials: R
authors.lastname: Souto
authors.forename: M
authors.initials: M
authors.lastname: de Uzeda
authors.forename: A P
authors.initials: AP
authors.lastname: Colombo
mesh_terms: D000190:Actinomyces / Q000235:genetics / Q000302:isolation & purification*; D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000032:analysis; D013293:Enterococcus faecalis / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010482:Periapical Abscess / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial