[Observation of female gametophyte development of Ginkgo biloba and its protoplast isolation and culture].
Results of observation showed that the female gametophyte of Ginkgo biloba was at the coenocytic stage from March 30ty to May 30ty. A density of 6-8 x 10(5) protoplasts/ml with a viability of 87.3% was achieved when the female gametopytes collected from May 8th to 15th were treated with 0.5% cellulase Onzuka R-10, 0.5% Pectolyase for 4-5 hours. The thin-layer liquid Murashige and Tuker medium modified by omitting ammonium ions and supplementing with glutamine 1000 mg/L, Vc 5 mg/L, benzyladenine 1.0 mg/L and naphthaleneacetic acid 3.0 mg/L was used for the protoplast culture and multiple cell colonies were obtained.
Chen
X S
XS
Shangdong Agricultural University, Taian 271018.
Deng
X X
XX
Zhang
W C
WC
Zhang
Y M
YM
D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D020441:Ginkgo biloba / Q000254:growth & development*; D006801:Humans; D011523:Protoplasts / Q000737:chemistry*
D003470:Culture Media