title: A simple, optimized method for the determination of sulphide in whole blood by GC-mS as a marker of bowel fermentation processes.
abstract: Hydrogen sulphide is produced in human large intestine by anaerobic fermentation and may play a pathogenic role. An analytical method for determination of sulphide in whole blood using an extractive alkylation technique was optimised and validated for this purpose. The sample was mixed with organic phase containing pentafluorobenzyl bromide as an alkylating agent. The benzalkonium chloride was used as a phase-transfer catalyst. The quantitative determination was performed using GC-MS technique in selected ion monitoring mode. The blood levels of sulphide of healthy controls were measured (35-80 microM/l). The method is versatile, reproducible (RSD=2.7%) and suitable for research of anaerobic fermentation in vivo.
authors.affiliation: Medical Faculty, Charles University, Hradec Králové, Czech Republic. rhyspler@lfhk.cuni.cz
authors.forename: Radomír
authors.initials: R
authors.lastname: Hyspler
authors.forename: Alena
authors.initials: A
authors.lastname: Tichá
authors.forename: Monika
authors.initials: M
authors.lastname: Indrová
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Zadák
authors.forename: Lidmila
authors.initials: L
authors.lastname: Hysplerová
authors.forename: Jirí
authors.initials: J
authors.lastname: Gasparic
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Churácek
mesh_terms: D015415:Biomarkers / Q000097:blood*; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007420:Intestine, Large / Q000502:physiology*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013440:Sulfides / Q000097:blood*
chemical_list: D015415:Biomarkers; D013440:Sulfides