title: Bioorganic mechanisms of the formation of free radicals catalyzed by glucose oxidase.
abstract: In this communication, we have described the activation of several xenobiotics by glucose oxidase from Aspergillus niger. The following compounds are readily reduced by d-glucose, in the presence of glucose oxidase: p-nitroso-N,N-dimethylaniline, methyl-1,4-benzoquinone, and 7,7,8,8-tetracyano-quinodimethane. In each case, the products of enzymatic reduction undergo a dismutation reaction with the parent compound and thus afford the formation of free radicals. In some cases, and at an appropriate pH value, the transformation of a parent compound into free radicals is almost quantitative. Under optimal conditions, free radicals are stable for several minutes in aqueous solutions under physiological conditions.
authors.affiliation: Faculty of Science Novi Sad, Yugoslavia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Trivić
authors.forename: Vladimir
authors.initials: V
authors.lastname: Leskovac
authors.forename: Jasmina
authors.initials: J
authors.lastname: Zeremski
authors.forename: Miroslav
authors.initials: M
authors.lastname: Vrvić
authors.forename: Gary W
authors.initials: GW
authors.lastname: Winston
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D016227:Benzoquinones / Q000737:chemistry / Q000378:metabolism; D002384:Catalysis; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009570:Nitriles / Q000737:chemistry / Q000378:metabolism; D009603:Nitroso Compounds / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D013379:Substrate Specificity; D015262:Xenobiotics / Q000737:chemistry / Q000378:metabolism
chemical_list: D016227:Benzoquinones; D005609:Free Radicals; D009570:Nitriles; D009603:Nitroso Compounds; D015262:Xenobiotics; C008651:4-nitrosodimethylaniline; C013703:tetracyanoquinodimethane; D005949:Glucose Oxidase; D005947:Glucose; C042889:2-methyl-1,4-benzoquinone