title: Purification and characterization of a laccase from the white-rot fungus Trametes multicolor.
abstract: The wood-degrading fungus Trametes multicolor secretes several laccase isoforms when grown on a simple medium containing copper in the millimolar range for stimulating laccase synthesis. The main isoenzyme laccase II was purified to apparent homogeneity from the culture supernatant by using anion-exchange chromatography and gel filtration. Laccase II is a monomeric glycoprotein with a molecular mass of 63 kDa as determined by sodium dodecylsulfate polyacrylamide gel electrophoresis, contains 18% glycosylation, and has a pI of 3.0. It oxidizes a variety of phenolic substrates as well as ferrocyanide and iodide. The pH optimum depends on the substrate employed and shows a bell-shaped pH activity profile with an optimum of 4.0 to 5.0 for the phenolic substrates, while the nonphenolic substrates ferrocyanide and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) show a monotonic pH profile with a rate decreasing with increasing pH.
authors.affiliation: Division of Biochemical Engineering, Institute of Food Technology, University of Agricultural Sciences Vienna, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Leitner
authors.forename: Johann
authors.initials: J
authors.lastname: Hess
authors.forename: Christiane
authors.initials: C
authors.lastname: Galhaup
authors.forename: Roland
authors.initials: R
authors.lastname: Ludwig
authors.forename: Bernd
authors.initials: B
authors.lastname: Nidetzky
authors.forename: Klaus D
authors.initials: KD
authors.lastname: Kulbe
authors.forename: Dietmar
authors.initials: D
authors.lastname: Haltrich
mesh_terms: D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D042845:Laccase; D008970:Molecular Weight; D010088:Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism*; D013379:Substrate Specificity; D013816:Thermodynamics
chemical_list: D007527:Isoenzymes; D010088:Oxidoreductases; D042845:Laccase