title: Evaluation of rapid DNA extraction methods for the quantitative detection of fungi using real-time PCR analysis.
abstract: Three comparatively rapid methods for the extraction of DNA from fungal conidia and yeast cells in environmental (air, water and dust) samples were evaluated for use in real-time PCR (TaqMan) analyses. A simple bead milling method was developed to provide sensitive, accurate and precise quantification of target organisms in air and water (tap and surface) samples. However, quantitative analysis of dust samples required further purification of the extracted DNA by a streamlined silica adsorption procedure.
authors.affiliation: National Exposure Research Laboratory, U.S. Environmental Protection Agency, 26 W. M.L. King Drive, Cincinnati, OH 45268, USA. haugland.rich@epa.gov
authors.forename: Richard A
authors.initials: RA
authors.lastname: Haugland
authors.forename: Nichole
authors.initials: N
authors.lastname: Brinkman
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Vesper
mesh_terms: D017931:DNA Primers; D004271:DNA, Fungal / Q000302:isolation & purification*; D005658:Fungi / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal