[The role of low-molecular-weight nitrogen compounds in the osmotolerance of Rhodococcus erythropolis and Arthrobacter globiformis].
Investigations showed that Rhodococcus erythropolis E-15 and Arthrobacter globiformis 2F cells respond to osmotic shock by increasing the synthesis of free amino acids, primarily glutamic acid (80% of the intracellular free amino acid pool). The osmoprotective role of glutamic acid follows from its beneficial effect on the growth of bacteria in high-salinity media. It was found that the addition of this amino acid to the growth medium at a concentration of 2 mM shortened the lag phase and increased the growth rate and biomass yield of either of the two bacteria. The addition of another osmoprotectant, trehalose, to the high-salinity growth medium of R. erythropolis E-15 at the same concentration (2 mM), restored the growth parameters of this bacterium to the control values.
Komarova
T I
TI
Department of Hydrobiology, Faculty of Biology, Moscow State University, Vorob'evy gory, Moscow, 119899 Russia.
Koronelli
T V
TV
Timokhina
E A
EA
D000596:Amino Acids / Q000096:biosynthesis*; D001173:Arthrobacter / Q000254:growth & development / Q000502:physiology*; D003470:Culture Media; D018698:Glutamic Acid / Q000096:biosynthesis; D017672:Nitrogen Compounds / Q000378:metabolism*; D009997:Osmotic Pressure; D012240:Rhodococcus / Q000254:growth & development / Q000502:physiology*; D012965:Sodium Chloride; D014199:Trehalose
D000596:Amino Acids; D003470:Culture Media; D017672:Nitrogen Compounds; D018698:Glutamic Acid; D012965:Sodium Chloride; D014199:Trehalose