7,8-Dihydroneopterin induces apoptosis of Jurkat T-lymphocytes via a Bcl-2-sensitive pathway.
Activated cell-mediated immunity is known to be accompanied by elevated concentrations of 7,8-dihydroneopterin which in high concentrations was found to interfere with the oxidant-antioxidant balance. In this study we investigated whether 7,8-dihydroneopterin mediates apoptosis of Jurkat T-lymphocytes via a CrmA- or Bcl-2-sensitive pathway. Transient transfection assays with CrmA and Bcl-2 expression constructs showed that apoptosis was not affected by CrmA whereas it was significantly decreased upon cotransfection with Bcl-2 constructs. Results suggest that 7,8-dihydroneopterin-induced apoptosis of T-lymphocytes is mediated by a Bcl-2-sensitive pathway.
Enzinger
Christiane
C
Institute for Medical Chemistry and Biochemistry, Innsbruck, Austria.
Wirleitner
Barbara
B
Lutz
Christina
C
Böck
Günther
G
Tomaselli
Bettina
B
Baier
Gottfried
G
Fuchs
Dietmar
D
Baier-Bitterlich
Gabriele
G
D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D019169:Jurkat Cells; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D019798:Neopterin / Q000031:analogs & derivatives; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D011621:Pteridines / Q000494:pharmacology*; D015843:Serpins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D019311:Staurosporine / Q000494:pharmacology; D014764:Viral Proteins
D000975:Antioxidants; D004791:Enzyme Inhibitors; D019253:Proto-Oncogene Proteins c-bcl-2; D011621:Pteridines; D015843:Serpins; D014764:Viral Proteins; C000976:7,8-dihydroneopterin; D019798:Neopterin; C074641:interleukin-1beta-converting enzyme inhibitor; D020928:Mitogen-Activated Protein Kinases; D019311:Staurosporine