title: Integration host factor interactions with Neisseria gene sequences: correlation between predicted binding sites and in vitro binding of Neisseria -derived IHF protein.
abstract: Putative integration host factor (IHF) binding sites are frequently being identified in Neisseria gene sequences on the basis of similarity to a degenerate Escherichia coli -derived consensus binding sequence. In this report, three different Neisseria genetic systems that contain predicted IHF binding sites were assessed for IHF binding through gel retardation analysis. The results show a positive correlation between the identification of a predicted Neisseria IHF binding site and in vitro binding of Neisseria -derived IHF protein.
authors.affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, US.
authors.forename: S A
authors.initials: SA
authors.lastname: Hill
authors.forename: D S
authors.initials: DS
authors.lastname: Samuels
authors.forename: C
authors.initials: C
authors.lastname: Nielsen
authors.forename: S W
authors.initials: SW
authors.lastname: Knight
authors.forename: F
authors.initials: F
authors.lastname: Pagotto
authors.forename: J A R
authors.initials: JA
authors.lastname: Dillon
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D034982:Integration Host Factors / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009343:Neisseria / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D016415:Sequence Alignment; D013223:Statistics as Topic
chemical_list: D004269:DNA, Bacterial; D034982:Integration Host Factors