title: Pituitary corticotroph adenoma containing many apoptotic cells: a histologic, immunohistochemical, ultrastructural and molecular study.
abstract: Apoptosis regulates cell turnover in normal tissues and occurs during the neoplastic process. Owing to difficulties in recognizing apoptotic cells by histology alone, several complementary approaches have been introduced, which disclosed the presence of cells with typical nuclear and cytoplasmic changes characteristic of apoptosis. Electron microscopy remains the most conclusive method to reveal the structural changes. Identification of caspase cleaved keratin 18 intermediate filament rearrangements can contribute to the identification of early apoptotic changes. The authors present here an unusual case of a pituitary corticotroph adenoma removed surgically from a young woman with Cushing disease. The tumor contained many apoptotic cells identified by histology. In addition, the apoptotic events were investigated using various morphologic techniques, including electron microscopy, the in situ end-labeling technique, and immunohistochemistry to confirm the caspase-cleaved keratin 18 rearrangements.
authors.affiliation: Department of Pathology, G. Gennimatas, Athens General Hospital, Greece.
authors.forename: Irene
authors.initials: I
authors.lastname: Messini
authors.forename: George
authors.initials: G
authors.lastname: Kontogeorgos
authors.forename: Kalman
authors.initials: K
authors.lastname: Kovacs
authors.forename: Eva
authors.initials: E
authors.lastname: Horvath
authors.forename: Harley S
authors.initials: HS
authors.lastname: Smyth
mesh_terms: D000236:Adenoma / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000328:Adult; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000032:analysis; D003480:Cushing Syndrome / Q000150:complications / Q000473:pathology / Q000601:surgery; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D020287:In Situ Nick-End Labeling; D019394:Ki-67 Antigen / Q000032:analysis; D010911:Pituitary Neoplasms / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D019394:Ki-67 Antigen; D000324:Adrenocorticotropic Hormone