title: Impaired light chain allelic exclusion and lack of positive selection in immature B cells expressing incompetent receptor deficient of CD19.
abstract: Positive signaling is now thought to be important for B cell maturation, although the nature of such signals has not yet been defined. We are studying the regulatory role of B cell Ag receptor (BCR) signaling in mediating positive selection of immature B cells. To do so, we use Ig transgenic mice (3-83Tg) that are deficient in CD19, thus generating a monoclonal immature B cell population expressing signaling-incompetent BCR. Immature 3-83Tg CD19(-/-) B cells undergo developmental arrest in the bone marrow, allowing maturation only to cells that effectively compensate for the compromised receptor by elevated levels of BCR. We find that developmentally arrested 3-83Tg CD19(-/-) B cells fail to impose L chain allelic exclusion and undergo intensive V(D)J recombination to edit their BCR. Furthermore, immature 3-83Tg CD19(-/-) B cells, which were grown in vitro, failed to undergo positive selection and to survive when adoptively transferred into normal recipients. However, elevation of BCR expression levels, obtained by transgene homozygosity, effectively compensated for the compromised BCR and completely restored BCR-mediated Ca(2+) influx, allelic exclusion, and positive selection. Our results suggest that the BCR signaling threshold mediates positive selection of developing B cells, and that a receptor-editing mechanism has an important role in rescuing cells that fail positive selection because of incompetent receptors.
authors.affiliation: Department of Immunology, Faculty of Medicine, Technion, and Endocrine Research Unit, Carmel Medical Center, Haifa, Israel.
authors.forename: Shoham
authors.initials: S
authors.lastname: Shivtiel
authors.forename: Nira
authors.initials: N
authors.lastname: Leider
authors.forename: Orit
authors.initials: O
authors.lastname: Sadeh
authors.forename: Zaki
authors.initials: Z
authors.lastname: Kraiem
authors.forename: Doron
authors.initials: D
authors.lastname: Melamed
mesh_terms: D000483:Alleles; D000818:Animals; D018941:Antigens, CD19 / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D015329:Gene Rearrangement, T-Lymphocyte; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D051379:Mice; D011947:Receptors, Antigen, B-Cell / Q000032:analysis / Q000502:physiology
chemical_list: D018941:Antigens, CD19; D007147:Immunoglobulin Light Chains; D011947:Receptors, Antigen, B-Cell; D002118:Calcium