title: Constituents of Chinese propolis and their antiproliferative activities.
abstract: Two new flavonoids, 3-O-[(S)-2-methylbutyroyl]pinobanksin (1) and 6-cinnamylchrysin (2), were isolated from the EtOAc-soluble fraction of the MeOH extract of Chinese propolis, along with 12 known compounds (3-14). The structures of the isolated compounds were elucidated on the basis of spectroscopic and chemical analyses. The isolated compounds were tested for their antiproliferative activity toward five different cancer cell lines. Benzyl caffeate (13) and phenethyl caffeate (14) showed potent antiproliferative activity toward tested cell lines with a selective activity toward colon 26-L5 carcinoma cell line (EC(50) values: 13, 1.01; 14, 0.30 microM).
authors.affiliation: Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630-Sugitani, Toyama 930-0194, Japan.
authors.forename: Tepy
authors.initials: T
authors.lastname: Usia
authors.forename: Arjun H
authors.initials: AH
authors.lastname: Banskota
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tezuka
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Midorikawa
authors.forename: Katsumichi
authors.initials: K
authors.lastname: Matsushige
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Kadota
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001713:Biphenyl Compounds; D002109:Caffeic Acids / Q000737:chemistry / Q000494:pharmacology; D002681:China; D002855:Chromatography, Thin Layer; D003110:Colonic Neoplasms; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D015394:Molecular Structure; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D011429:Propolis / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: C458536:3-O-(2-methylbutyroyl)pinobanksin; C458537:6-cinnamylchrysin; D000970:Antineoplastic Agents; D001713:Biphenyl Compounds; D002109:Caffeic Acids; D005419:Flavonoids; D016166:Free Radical Scavengers; D010851:Picrates; C115232:benzyl caffeate; D011429:Propolis; C004931:1,1-diphenyl-2-picrylhydrazyl; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol