Cloning of a tobacco cDNA coding for a putative transcriptional coactivator MBF1 that interacts with the tomato mosaic virus movement protein.
Viral movement through plasmodesmata in host plants depends on the interaction between virus-encoded movement protein (MP) and host proteins. To search for MP-interacting protein (MIP), far-western screening of a tobacco cDNA library was carried out using a recombinant MP of tomato mosaic virus (ToMV) as a probe. One of the positive cDNA clones, designated MIP204, was highly homologous to a class of transcriptional coactivators commonly referred to as multiprotein bridging factor 1 (MBF1). ToMV MP could also bind to the Arabidopsis homologues of MBF1. The recombinant MIP204 bound to MPs of ToMV and a crucifer tobamovirus CTMV-W, but not of cucumber mosaic virus. MPs of ToMV and the related virus may interact with MBF1-like proteins to modulate host gene expression.
Matsushita
Yasuhiko
Y
Gene Research Center, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Miyakawa
Orie
O
Deguchi
Masakazu
M
Nishiguchi
Masamichi
M
Nyunoya
Hiroshi
H
D029681:Arabidopsis Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D053830:Plant Viral Movement Proteins; D011485:Protein Binding; D014026:Nicotiana / Q000235:genetics*; D014027:Tobacco Mosaic Virus / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; C491466:MBF1 protein, Arabidopsis; D053830:Plant Viral Movement Proteins; D015534:Trans-Activators; D014764:Viral Proteins