Proteomic map and database of lymphoblastoid proteins.
Advances in genomics have led to the accumulation of an unprecedented amount of data, giving rise to a new field in biochemistry, proteomics. We used a combination of two dimensional gel electrophoresis, analysis and annotation using third-generation software, and mass spectrometry to establish the proteome maps of lymphoblastoid B-cells, a prerequisite for analysis of drug effects and lymphocyte cell diseases. About 1200 protein spots were detected and characterised in terms of their isoelectric point, molecular mass and expression. The present status of proteomic technologies, as well as a description of the usefulness of human hematopoietic cells proteomic database are discussed.
Caron
Michel
M
Université Paris 13, UFR SMBHI Leonard de Vinci, Bobigny, France. caron@smbh.univ-paris13.fr
Imam-Sghiouar
Naima
N
Poirier
Florence
F
Le Caër
Jean-Pierre
JP
Labas
Valerie
V
Joubert-Caron
Raymonde
R
D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel; D008214:Lymphocytes / Q000737:chemistry*; D020543:Proteome
D020543:Proteome