Artifactual changes in equine blood following storage, detected using the Advia 120 hematology analyzer.
BACKGROUND
Delayed analysis of blood samples may be caused by restricted access to laboratories. Artifactual changes may occur in the measured analytes as a consequence of delayed analysis and may complicate interpretation of the data.


OBJECTIVE
The purpose of this study was to characterize artifactual changes in equine blood, due to storage, using the Advia 120 hematology analyzer.


METHODS
Samples of blood from 5 horses were analyzed using the Advia 120 soon after collection and again after 24 and 48 hours of storage at either 4 degrees C or ambient laboratory temperature ( approximately 24 degrees C).


RESULTS
Delayed analysis of equine blood samples resulted in increased numbers of normocytic hypochromic RBCs, increased numbers of macrocytic hypochromic RBCs, misclassification of granulocytes as mononuclear cells using the basophil reagent method, and pseudothrombocytosis, due to misclassification of ghost RBCs as platelets. The latter artifact was corrected by an amended version of the software. Many of the artifactual changes were identified by morphology flags.


CONCLUSIONS
Characteristic changes in cytograms produced by the Advia 120 allowed recognition of artifactual changes in stored equine blood samples. These changes were less pronounced in samples stored at 24 degrees C than at 4 degrees C.
Clark
Phillip
P
Pathobiology Section, Institute of Veterinary, Animal, and Biochemical Sciences, Massey University, Palmerston North, New Zealand. pclark@murdoch.edu.au
Mogg
Tony D
TD
Tvedten
Harold W
HW
Korcal
David
D
D000818:Animals; D016477:Artifacts; D001793:Blood Preservation / Q000379:methods / Q000662:veterinary*; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000502:physiology; D005260:Female; D006403:Hematologic Tests / Q000295:instrumentation / Q000592:standards / Q000662:veterinary*; D006736:Horses / Q000097:blood*; D008297:Male; D013696:Temperature; D013997:Time Factors