Evaluation of rapid DNA extraction methods for the quantitative detection of fungi using real-time PCR analysis.
Three comparatively rapid methods for the extraction of DNA from fungal conidia and yeast cells in environmental (air, water and dust) samples were evaluated for use in real-time PCR (TaqMan) analyses. A simple bead milling method was developed to provide sensitive, accurate and precise quantification of target organisms in air and water (tap and surface) samples. However, quantitative analysis of dust samples required further purification of the extracted DNA by a streamlined silica adsorption procedure.
Haugland
Richard A
RA
National Exposure Research Laboratory, U.S. Environmental Protection Agency, 26 W. M.L. King Drive, Cincinnati, OH 45268, USA. haugland.rich@epa.gov
Brinkman
Nichole
N
Vesper
Stephen J
SJ
D017931:DNA Primers; D004271:DNA, Fungal / Q000302:isolation & purification*; D005658:Fungi / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
D017931:DNA Primers; D004271:DNA, Fungal