title: A single amino acid substitution in 126-kDa protein of Pepper mild mottle virus associates with symptom attenuation in pepper; the complete nucleotide sequence of an attenuated strain, C-1421.
abstract: The complete nucleotide sequence of an attenuated Pepper mild mottle virus (PMMoV C-1421) RNA genome has been determined. There were two differences from the type isolate in Japan (PMMoV-J). The mutations were located in the middle of the 126-kDa protein (126 K) gene; one mutation influenced amino acid substitution at 649th Val to Ala (V649A), and the other was silent. The analyses using the reverse genetic system of PMMoV-J revealed that symptom attenuation on pepper related to V649A. Accumulations of 126 K and coat protein (CP) in V649A mutant-infected pepper were lower than those of PMMoV-J in immunoblotting. These results suggest that V649A substitution in 126 K affects the accumulation of 126 K leading to a limitation of CP accumulation.
authors.affiliation: National Agricultural Research Center, Tsukuba, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hagiwara
authors.forename: T U
authors.initials: TU
authors.lastname: Ichiki
authors.forename: Y
authors.initials: Y
authors.lastname: Ogawa
authors.forename: T
authors.initials: T
authors.lastname: Omura
authors.forename: S
authors.initials: S
authors.lastname: Tsuda
mesh_terms: D019943:Amino Acid Substitution; D002212:Capsicum / Q000191:economics / Q000821:virology*; D002213:Capsid / Q000032:analysis / Q000096:biosynthesis; D016679:Genome, Viral; D015151:Immunoblotting; D007564:Japan; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000191:economics / Q000821:virology*; D012324:RNA-Dependent RNA Polymerase / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D017898:Tobamovirus / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D014633:Valine / Q000235:genetics
chemical_list: D012324:RNA-Dependent RNA Polymerase; D014633:Valine