title: NADH oxidation by manganese peroxidase with or without alpha-hydroxy acid.
abstract: NADH oxidation by manganese peroxidase (MnP) was done in a reaction mixture including either alpha-hydroxy acid or acetate. The oxidation in the former reaction mixture was inhibited by a catalase and was accelerated by exogenous H2O2, while the oxidation in the latter reaction mixture was inhibited by a superoxide dismutase and was not accelerated by the exogenous H2O2. These results indicated that there are significant differences between the two reaction systems, particularly, in the active oxygen species involved in the reactions. Additionally, the experiment of MnP reduction with Mn(II) suggests that MnP has a separate catalytic activity other than an oxidation of Mn(II) to Mn(III) in the reaction mixture including acetate.
authors.affiliation: Chemical and Environmental Technology Laboratory, Kobe Steel, Ltd., Japan. te-deguchi@rd.kcrl.kobelco.co.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Deguchi
authors.forename: Masaaki
authors.initials: M
authors.lastname: Matsubara
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Nishida
mesh_terms: D000085:Acetates / Q000494:pharmacology; D005658:Fungi / Q000201:enzymology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006880:Hydroxy Acids / Q000494:pharmacology*; D007700:Kinetics; D008293:Malates / Q000494:pharmacology; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*
chemical_list: D000085:Acetates; D006880:Hydroxy Acids; D008293:Malates; D009243:NAD; D006861:Hydrogen Peroxide; D010544:Peroxidases; C051129:manganese peroxidase