The influence of ovariectomy and estrogen replacement on voiding patterns and detrusor muscarinic receptor affinity in the rat.
A rat model of ovariectomy-induced voiding dysfunction was established and the effects of ovariectomy and subsequent estrogen replacement on the affinity of muscarinic receptors in the rat bladder were determined. Voiding frequency and spatial distribution patterns were documented in sham-operated (control), and ovariectomized (placebo- or estrogen-treated) rats. The ovariectomized rats had a significantly different urinating pattern, i.e. higher voiding frequency and less peripheral voiding than the sham-operated group, suggestive of urge incontinence. Using this model of voiding dysfunction, negative logs of dissociation constants of carbachol of the rat detrusor muscarinic receptors were then determined indirectly using the Furchgott's double-reciprocal method. Receptor affinities were not significantly different in all groups compared to control females. In conclusion, a model of ovariectomy-induced voiding dysfunction in ovariectomized rats was established, where bladder dysfunction occurred with no significant changes in the affinity of muscarinic receptors.
Liang
Willmann
W
Department of Pharmacology and Therapeutics, 2176 Health Sciences Mall, University of British Columbia, V6T 1Z3, Vancouver, BC, Canada.
Afshar
Karoush
K
Stothers
Lynn
L
Laher
Ismail
I
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D018678:Cholinergic Agents / Q000494:pharmacology; D015914:Estrogen Replacement Therapy; D005260:Female; D008297:Male; D008593:Menopause / Q000378:metabolism; D023421:Models, Animal; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism*
D018678:Cholinergic Agents; D011976:Receptors, Muscarinic; D002217:Carbachol