Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1.
An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.
Mukai
Tetsu
T
Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
Komoto
Satoshi
S
Kurosu
Takeshi
T
Palacios
J Alejandro
JA
Li
Yong-Gang
YG
Auwanit
Wattana
W
Tatsumi
Masashi
M
Ikuta
Kazuyoshi
K
D001483:Base Sequence; D018791:CD4 Lymphocyte Count; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D014774:Virulence
D017931:DNA Primers