title: DDA adjuvant induces a mixed Th1/Th2 immune response when associated with BBG2Na, a respiratory syncytial virus potential vaccine.
abstract: Human respiratory syncytial virus (hRSV) is one of the most common causes of respiratory infection in infants and the elderly. Previous attempts to vaccinate children against RSV failed and the induction of an aberrant Th2-type immune response was shown to induce severe to fatal pulmonary disease characterised in part by eosinophilia. BBG2Na is a promising human RSV subunit vaccine candidate which successfully passed phase II clinical trials in adults in association with Adju-Phos((R)). However, this formulation is not the most suitable for use in children since aluminium salts are known to induce a Th2-based immune response. In this study, we describe a potent and safe adjuvant formulation for BBG2Na in dimethyldioctadecylammonium bromide (DDA) that induces a mixed Th1/Th2 immune response in BALB/c mice. Furthermore, BBG2Na showed the same protective efficacy against RSV challenge when formulated either in DDA or in alum in mice and cotton rats.
authors.affiliation: Centre d'Immunologie Pierre Fabre, 5 Avenue Napoléon III, BP 497, F-74164 Saint Julien-en-Genevois, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Klinguer-Hamour
authors.forename: Christine
authors.initials: C
authors.lastname: Libon
authors.forename: Hélène
authors.initials: H
authors.lastname: Plotnicky-Gilquin
authors.forename: Marie Claire
authors.initials: MC
authors.lastname: Bussat
authors.forename: Lydie
authors.initials: L
authors.lastname: Revy
authors.forename: Thien
authors.initials: T
authors.lastname: Nguyen
authors.forename: Jean Yves
authors.initials: JY
authors.lastname: Bonnefoy
authors.forename: Nathalie
authors.initials: N
authors.lastname: Corvaïa
authors.forename: Alain
authors.initials: A
authors.lastname: Beck
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D051381:Rats; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage* / Q000009:adverse effects / Q000276:immunology*; D016205:Sigmodontinae; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000009:adverse effects / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; C472186:BBG2Na vaccine; D015848:Interleukin-5; D000644:Quaternary Ammonium Compounds; D022261:Respiratory Syncytial Virus Vaccines; D019444:Vaccines, DNA; D014764:Viral Proteins; D015847:Interleukin-4; C015831:dimethyldioctadecylammonium; D007371:Interferon-gamma