Oxidative biotransformations using oxygenases.
Considerable progress has been made in manipulating oxidative biotransformations using oxygenases. Substrate acceptance, catalytic activity, regioselectivity and stereoselectivity have been improved significantly by substrate engineering, enzyme engineering or biocatalyst screening. Preparative biotransformations have been carried out to synthesize useful pharmaceutical intermediates or chiral synthons on the gram to several-hundred-gram scale, by use of whole cells of wild type or recombinant strains. The synthetic application of oxygenases in vitro has been shown to be possible by enzymatic or electrochemical regeneration of NADH or NADPH.
Li
Zhi
Z
Institute of Biotechnology, ETH Zurich, Hoenggerberg, CH-8093, Zurich, Switzerland. zhi@biotech.biol.ethz.ch
van Beilen
Jan B
JB
Duetz
Wouter A
WA
Schmid
Andreas
A
de Raadt
Anna
A
Griengl
Herfried
H
Witholt
Bernard
B
D001711:Biotransformation / Q000187:drug effects; D003067:Coenzymes; D006900:Hydroxylation; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D015202:Protein Engineering; D013379:Substrate Specificity; D014169:Transformation, Bacterial
D003067:Coenzymes; D010105:Oxygenases