title: STAG2 and Rad21 mammalian mitotic cohesins are implicated in meiosis.
abstract: STAG/SA proteins are specific cohesin complex subunits that maintain sister chromatid cohesion in mitosis and meiosis. Two members of this family, STAG1/SA1 and STAG2/SA2,double dagger are classified as mitotic cohesins, as they are found in human somatic cells and in Xenopus laevis as components of the cohesin(SA1) and cohesin(SA2) complexes, in which the shared subunits are Rad21/SCC1, SMC1 and SMC3 proteins. A recently reported third family member, STAG3, is germinal cell-specific and is a subunit of the meiotic cohesin complex. To date, the meiosis-specific cohesin complex has been considered to be responsible for sister chromatid cohesion during meiosis. We studied replacement of the mitotic by the meiotic cohesin complex during mouse germinal cell maturation, and we show that mammalian STAG2 and Rad21 are also involved in several meiosis stages. Immunofluorescence results suggest that a cohesin complex containing Rad21 and STAG2 cooperates with a STAG3-specific complex to maintain sister chromatid cohesion during the diplotene stage of meiosis.
authors.affiliation: Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, UAM Campus de Cantoblanco, Madrid E-28049, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Prieto
authors.forename: Nieves
authors.initials: N
authors.lastname: Pezzi
authors.forename: Jose M
authors.initials: JM
authors.lastname: Buesa
authors.forename: Leonor
authors.initials: L
authors.lastname: Kremer
authors.forename: Isabel
authors.initials: I
authors.lastname: Barthelemy
authors.forename: Candelas
authors.initials: C
authors.lastname: Carreiro
authors.forename: Fernando
authors.initials: F
authors.lastname: Roncal
authors.forename: Alicia
authors.initials: A
authors.lastname: Martinez
authors.forename: Lucio
authors.initials: L
authors.lastname: Gomez
authors.forename: Raul
authors.initials: R
authors.lastname: Fernandez
authors.forename: Carlos
authors.initials: C
authors.lastname: Martinez-A
authors.forename: Jose L
authors.initials: JL
authors.lastname: Barbero
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D005455:Fluorescent Antibody Technique; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008938:Mitosis / Q000502:physiology; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D010750:Phosphoproteins / Q000235:genetics / Q000502:physiology*; D011418:Prophase / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins / Q000502:physiology; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D029702:Schizosaccharomyces pombe Proteins; C465862:Rad21 protein, mouse; C476538:Stag2 protein, mouse; C498637:Stag3 protein, mouse; C077081:REC8 protein, S pombe; D000097722:Cohesins