Liquid core waveguide for full imaging of electrophoretic separations.
We employed a liquid core waveguide to image both DNA electrophoresis separations and isoelectric focusing of proteins. The utility of the system is demonstrated for DNA fragment sizing and protein separations. The system utilizes the liquid-core waveguide as an efficient window for both the excitation of separated samples and the collection of light through total internal reflectance, with an ability to detect target molecules in the zeptomolar range. Scanning the excitation laser along the length of the electrophoresis capillary excites individually separated analyte bands, while the fluorescence is collected end-on by an optical fiber coupled to a photomultiplier, thus, creating an image of the separation along the length of the capillary.
Olivares
José A
JA
Los Alamos National Laboratory, Bioscience Division, New Mexico 87545, USA.
Stark
Peter C
PC
Jackson
Paul
P
D004247:DNA / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted / Q000379:methods
D004247:DNA