Stability of a recombinant adenoviral vector: optimization of conditions for storage, transport and delivery.
Recombinant viral vectors have been developed for use as therapeutic agents and for the introduction of exogenous genes into living cells. However, little is known about the viability and stability of such recombinant viruses during storage, transport and delivery under various conditions. We describe here an analysis of the stability of an adenoviral vector in crude solutions of cell lysates during freezing and thawing and during storage at various temperatures in the presence and in the absence of glycerol. For example, the titer of adenoviruses in crude lysates of infected cells was reduced only ten-fold or three-fold after two hundred rounds of freezing and thawing or after incubation at 28 degrees C for 14 days, respectively. Our observations indicate that recombinant adenoviral vector was more stable than expected both during freezing and thawing and during storage at low temperatures. Our results confirm the importance of appropriate conditions for the delivery and transport of recombinant adenoviral vectors.
Ugai
Hideyo
H
Gene Engineering Division, Department of Biological Systems, BioResource Center, Tsukuba Institute, RIKEN, Tsukuba, Ibaraki 305-0074.
Watanabe
Sanae
S
Suzuki
Erika
E
Tsutsui-Nakata
Hatsumi
H
Yokoyama
Kazunari K
KK
Murata
Takehide
T
D000256:Adenoviridae / Q000235:genetics*; D002460:Cell Line; D005615:Freezing; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D013048:Specimen Handling; D013696:Temperature; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
D001616:beta-Galactosidase