Separation of phospholipid classes in sea urchin, paracentrotus lividus by high-performance liquid chromatography.
A simple high-performance liquid chromatography (HPLC) method for determination of major phospholipid classes in sea urchin Paracentrotus lividus is described. The separation was performed on a Tracer Extrasil SI 5 microm 25 x 0.4 cm column and an isocratic mobile phase of acetonitrile-methanol 85%-phosphoric acid (50:50:1.8, v/v). The HPLC method utilizes UV detection at 205 nm. Five phospholipids were identified and quantified: phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC) and sphingomyelin (SM). Fresh and canned samples were analyzed. Student's t-test showed no significant difference (P < or = 0.05) between the mean phospholipid contents of raw and canned sea urchin.
Rodriguez-Bernaldo de Quiros
A
A
Nutrition and Bromatology Department, Pharmacy Faculty, University of Santiago de Compostela, La Coruña, Spain.
López-Hernández
J
J
Simal-Lozano
J
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010743:Phospholipids / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012617:Sea Urchins; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D010743:Phospholipids