title: Inhibitory effect of natto, a kind of fermented soybeans, on LDL oxidation in vitro.
abstract: The oxygen radical scavenging activity of natto (fermented soybeans) and its inhibitory effect on the oxidation of rat plasma low-density lipoprotein (LDL) in vitro were investigated to evaluate the usefulness of the antioxidant properties of natto, which has been shown to have antioxidant activity. Natto was separated into three water-soluble fractions: high-molecular-weight viscous substance (HMWVS; Mw > 100 000), low-molecular-weight viscous substance (LMWVS; Mw < 100 000), and soybean water extract (SWE). LMWVS had the strongest radical scavenging activity for hydroxyl and superoxide anion radicals, as assessed by electron spin resonance. The increase of conjugated dienes in LDL oxidized by copper and an azo pigment was depressed by the addition of LMWVS and SWE. These results demonstrate that natto fractions have inhibitory effects on LDL oxidation as a result of their radical scavenging activity.
authors.affiliation: Biotechnology Section, Aomori Advanced Industrial Technology Center, 4-11-6, Daini-tonyamachi, Aomori 030-0113, Japan. iwai@aomori-tech.go.jp
authors.forename: Kunihisa
authors.initials: K
authors.lastname: Iwai
authors.forename: Natsuko
authors.initials: N
authors.lastname: Nakaya
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawasaki
authors.forename: Hajime
authors.initials: H
authors.lastname: Matsue
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001391:Azo Compounds / Q000737:chemistry; D001412:Bacillus subtilis / Q000378:metabolism; D003300:Copper / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D005285:Fermentation; D016166:Free Radical Scavengers / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry; D008297:Male; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013025:Glycine max / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry; D014867:Water
chemical_list: D000975:Antioxidants; D001391:Azo Compounds; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D010936:Plant Extracts; D014867:Water; D013481:Superoxides; D017665:Hydroxyl Radical; D003300:Copper