title: Effects of melatonin on noncardiogenic pulmonary edema secondary to adnexial ischemia-reperfusion in guinea pig.
abstract: OBJECTIVE
  Pulmonary edema has been shown to occur following ischemia-reperfusion injury in a variety of organs and effects of several pharmacological agents on ischemia-reperfusion-induced damage has been investigated previously. However, there are only a few studies in the literature about pulmonary injury following adnexial ischemia-reperfusion. In this study we aimed at investigating pulmonary changes following adnexial ischemia-reperfusion and the effects of melatonin on noncardiogenic pulmonary edema secondary to adnexial ischemia-reperfusion.
  
  
  METHODS
  A total of 32 Dunkin-Harley guinea pigs were randomly divided into four groups. In group I, oopherectomy was performed following adnexial torsion of three hours (ischemia). Adnexial torsion of three hours followed by a 3 hours detorsion period (reperfusion) and then oopherectomy was performed in remaining three groups. No therapy was given in group one and two while isotonic saline and melatonin was applied intraperitoneally in groups three and four, respectively. Serum malondialdehyde (MDA) levels of operated ovaries and lungs and polimorphonuclear leucocyte infiltration of lungs were determined.
  
  
  RESULTS
  MDA levels in serum, ovary and lungs were higher in detorsion groups (groups II, III) than torsion group (group I) (p<0.01). Melatonin administration significantly decreased the polimorphonuclear leukocytes infiltration of lung parenchime and MDA levels in serum, ovaries and lungs (groups II and IV; groups III and IV; p<0.01, p<0.01). MDA levels and lung tissue PNL infiltration levels of melatonin administered detorsioned group was similar to those levels of only salphingo-oopherectomy performed group (Group I and IV, p>0.05).
  
  
  CONCLUSIONS
  Pathophysiology of ischemia-reperfusion must be considered in the cases of adnexial torsion where detorsion is thought. As an antioxidant, melatonin administration might be helpful in decreasing post-operative morbidity by decreasing reperfusion injury of lungs.
authors.affiliation: Firat University, Faculty of Medicine, Department of Obstetrics and Gynecology, Elazig, Turkey.
authors.forename: Husnu
authors.initials: H
authors.lastname: Celik
authors.forename: Ahmet
authors.initials: A
authors.lastname: Ayar
authors.forename: Niyazi
authors.initials: N
authors.lastname: Tug
authors.forename: Mehmet
authors.initials: M
authors.lastname: Simsek
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Ozercan
authors.forename: Gürkan
authors.initials: G
authors.lastname: Cikim
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Bildirici
mesh_terms: D000291:Adnexal Diseases / Q000150:complications / Q000188:drug therapy; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D008168:Lung / Q000737:chemistry / Q000473:pathology; D008315:Malondialdehyde / Q000032:analysis; D008550:Melatonin / Q000494:pharmacology*; D010052:Ovariectomy; D011654:Pulmonary Edema / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D014102:Torsion Abnormality
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D008550:Melatonin