Thermoresponsive artificial extracellular matrix: N-isopropylacrylamide-graft-copolymerized gelatin.
Poly(N-isopropylacrylamide)-graft-copolymerized gelatin (PNIPAM-gelatin) was prepared by iniferter-based photopolymerization of multiply derivatized dithiocarbamylated gelatin. PNIPAM-gelatins exhibited low critical solution temperature (LCST) immediately below the physiological temperature. PNIPAAm-gelatin-coated dishes induced cell adhesion at 37 degrees C but incomplete detachment at room temperature, whereas dishes coated with PNIPAM or a mixture of PNIPAAm and gelatin showed little cell adhesion. The mixture of PNIPAAm-gelatin and PNIPAAm induced cell adhesion at 37 degrees C and detachment at 20 degrees C: the degrees of cell adhesion and detachment depended on the mixed ratio of PNIPAAm-gelatin and PNIPAAm. Complete thermoresponsive adhesion and detachment were found for the mixture containing a small fraction of PNIPAAm-gelatin (approximately 5 wt% with respect to PNIPAAm; gelatin content in the mixture is 2.7 wt%). Such a mixture may serve as thermoresponsive cell matrix for fabrication of a tissue-engineered device.
Morikawa
Nobuyuki
N
Department of Bioengineering, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
Matsuda
Takehisa
T
D000178:Acrylamides / Q000494:pharmacology*; D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001011:Aorta / Q000378:metabolism; D001565:Benzoates / Q000737:chemistry; D001672:Biocompatible Materials / Q000494:pharmacology; D002417:Cattle; D002448:Cell Adhesion; D002478:Cells, Cultured; D005780:Gelatin / Q000737:chemistry* / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D000178:Acrylamides; D000180:Acrylic Resins; D001565:Benzoates; D001672:Biocompatible Materials; D011108:Polymers; C052970:poly-N-isopropylacrylamide; D005780:Gelatin; C067295:N-isopropylacrylamide