Sensitive liquid chromatographic--mass spectrometric assay for norfloxacin in poultry tissue.
A highly sensitive and specific method for the determination of norfloxacin in poultry tissues by LC-MS was developed and validated. An extract of the sample was separated on a C(18) reversed-phase column and analyzed by LC-MS. The mobile phase was gradiently flowed with 2% acetic acid and acetonitrile. The limit of detection and limit of quantitation were 1 and 5 ng/g, respectively. Mean recoveries from various spiked tissues were 87.2% (ranging from 82.5 to 92.7%) for norfloxacin. The method has been successfully applied to determine norfloxacin in poultry muscle.
Lim
Jonghwan
J
Division of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chungnam National University, 220 Kung-dong, Yusong-ku, Taejon, 305-764, South Korea.
Park
Byungkwon
B
Yun
Hyoin
H
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D009643:Norfloxacin / Q000032:analysis*; D011200:Poultry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000890:Anti-Infective Agents; D009643:Norfloxacin