3-[123I]Iodo-L-alpha-methyl tyrosine transport into human fibroblasts and comparison with Ewing's sarcoma cells.
The cellular transport systems and the transport kinetics of [123I]IMT uptake into non-malignant extracranial cells were characterized for the first time. Human fibroblasts were chosen as non-malignant extracranial cells as they are found ubiquitous in the body. [123I]IMT is exclusively transported into fibroblasts via the sodium independent system L. An apparent Michaelis constant K(m) = 116.2 +/- 18.9 microM and a maximum transport velocity V(max) = 191.6 +/- 13.9 pmol x (10(6) cells)(-1) x min(-1) were calculated for the sodium-independent transport. These results were compared with those determined in two malignantly transformed extracranial cell lines, the human Ewing's sarcoma cell lines VH-64 and CADO-ES-1.
Franzius
Christiane
C
Department of Nuclear Medicine, University Hospital, Muenster, Germany. franziu@uni-muenster.de
Kopka
Klaus
K
van Valen
Frans
F
Riemann
Burkhard
B
Sciuk
Joachim
J
Schober
Otmar
O
D001859:Bone Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism*; D002460:Cell Line; D005347:Fibroblasts / Q000000981:diagnostic imaging / Q000378:metabolism*; D006801:Humans; D008781:Methyltyrosines / Q000493:pharmacokinetics*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012512:Sarcoma, Ewing / Q000000981:diagnostic imaging / Q000378:metabolism*; D012680:Sensitivity and Specificity; D012964:Sodium / Q000493:pharmacokinetics
D008781:Methyltyrosines; D019275:Radiopharmaceuticals; D012964:Sodium; C030855:3-iodo-alpha-methyltyrosine