title: Immunogenicity of a recombinant lumpy skin disease virus (neethling vaccine strain) expressing the rabies virus glycoprotein in cattle.
abstract: Rabies virus (RV) readily infects cattle and causes a fatal neurological disease. A stable vaccine, which does not require the maintenance of a cold chain and that is administered once to elicit lifelong immunity to rabies would be advantageous. The present study describes the construction of a live recombinant lumpy skin disease virus (LSDV) vaccine, expressing the glycoprotein of rabies virus (RG) and assessment of its ability to generate a humoral and cellular immune response against rabies virus in cattle. Cattle inoculated with the recombinant virus (rLSDV-RG) developed humoral immunity that was demonstrated in ELISA and neutralisation assays to RV. High titres of up to 1513IU/ml of RV neutralising antibodies were induced. In addition, peripheral blood mononuclear cells from rLSDV-RG-immunised animals demonstrated the ability to proliferate in response to stimulation with inactivated RV, whereas the animal vaccinated with wild type LSDV did not. This recombinant vaccine candidate thus has the potential to be used in ruminants as a cost-effective vaccine against both lumpy skin disease (LSD) and rabies.
authors.affiliation: Division of Medical Virology, Department Clinical Laboratory Sciences, Faculty of Health Sciences, University of Cape Town, Observatory, Cape Town 7925, South Africa.
authors.forename: Kate
authors.initials: K
authors.lastname: Aspden
authors.forename: Alberdina A
authors.initials: AA
authors.lastname: van Dijk
authors.forename: John
authors.initials: J
authors.lastname: Bingham
authors.forename: Dermot
authors.initials: D
authors.lastname: Cox
authors.forename: Jo-Ann
authors.initials: JA
authors.lastname: Passmore
authors.forename: Anna-Lise
authors.initials: AL
authors.lastname: Williamson
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral; D002417:Cattle; D018929:Cell Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D017930:Genes, Reporter; D005822:Genetic Vectors; D006023:Glycoproteins / Q000276:immunology*; D007111:Immunity, Cellular; D008167:Lumpy skin disease virus / Q000235:genetics / Q000276:immunology*; D011819:Rabies Vaccines / Q000276:immunology*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D011819:Rabies Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus; D001616:beta-Galactosidase