[Identification of a protein interacting with apoptin from human leucocyte cDNA library by using yeast two-hybrid screening].
To screen the protein interacting with apoptin from human leucocyte cDNA library by using yeast two-hybrid system, four clones interacting with apoptin were identified. One of them was homologous with Nmi (N-Myc interaction protein). Cell co-immunoprecipitation showed that apoptin could bind to Nmi in mammalian cells. Apoptin mutants T1, T2 and T3 lacked the C-terminal 11 AA,33-46 AA and both,respectively. Apoptin mutants T2 and T3 failed to interact with Nmi, suggesting that its 33-46 AA was pivotal for the interaction. Apoptin mutant T1 still interacted with Nmi, suggesting that its C-terminal 11 AA was not essential for the interaction.
Sun
G J
GJ
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China. sunzx@nic.bmi.ac.cn
Tong
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Dong
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Mei
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Sun
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D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007962:Leukocytes / Q000378:metabolism*; D009154:Mutation; D011233:Precipitin Tests; D012441:Saccharomyces cerevisiae; D020798:Two-Hybrid System Techniques
D036022:Capsid Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C099927:NMI protein, human; C091717:VP3 protein, Chicken anemia virus