An Expression Release System for Heterogeneous Proteins in E.coli Mediated by the kil Gene.
An expression release system for heterogeneous proteins in Escherichia coli mediated by the colicin release gene(kil gene) was constructed. The system is based on the ability of the Kil protein to release periplasmic proteins into the growth medium. beta-lactamase, an E.coli periplasmic protein, and prolyl endopeptidase (PEP), a periplasmic protein of Aeromonas punctata subsp. punctata were used as report proteins. Commonly, these two proteins are seldom released into the growth medium. The results indicated that the released amounts of the beta-lactamase and of the prolyl endopeptidase were nearly 5 fold and 4 fold than control, respectively.
Tong
Qin
Q
Research Center of Biotechnology, Shanghai Institute for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200233, China. ygong@srcb.ac.cn
Zhang
Hui-Tang
HT
Yang
Yun-Gui
YG
Yang
Sheng-Li
SL
Gong
Yi
Y