Characterization of arsenic species in kidney of the clam Tridacna derasa by multidimensional liquid chromatography-ICPMS and electrospray time-of-flight tandem mass spectrometry.
The identification of water-soluble arsenic species in a kidney of the Tridacna clam by electrospray quadrupole/time-of-flight mass spectrometry (ES Q-TOF MS) was investigated. The species were isolated by three-dimensional LC (size exclusion-anion exchange-cation exchange); the elution of arsenic was monitored by ICPMS. The average accuracy and precision of the molecular mass measurements, studied for a number of organoarsenic standards, were 22 (negative bias) and 15 ppm, respectively. Structural information was obtained in the tandem Q-TOF mode. A total of 15 organoarsenic species were identified, and 13 of these possessed the dimethylarsinoyl group (8 ribofuranosides, 4 acyclic compounds, and 1 dihydroxyfuran). Four of these species were previously unreported. Arsenobetaine and dimethylarsinic acid were also detected. The major species (accounting for up to 50% water-soluble arsenic) was 5-dimethylarsinoyl-2,3,4-trihydroxypentanoic acid. The metabolic interrelationships of these compounds and their significance are briefly discussed.
McSheehy
Shona
S
Group of Bio-inorganic Analytical Chemistry, CNRS UMR 5034, Hélioparc, France.
Szpunar
Joanna
J
Lobinski
Ryszard
R
Haldys
Violette
V
Tortajada
Jeanine
J
Edmonds
John S
JS
D000818:Animals; D001152:Arsenicals / Q000032:analysis* / Q000493:pharmacokinetics; D001711:Biotransformation; D049872:Bivalvia / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007668:Kidney / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D001152:Arsenicals