title: Purification and characterization of a novel polysaccharide involved in the pellicle produced by a thermotolerant Acetobacter strain.
abstract: Acetobacter strains able to produce a thick pellicle at 37 degrees C were screened among many thermotolerant strains isolated from fruits in Thailand. As a result, Acetobacter sp. SKU 1100 was selected as the producer of a relatively thick pellicle even when cultured at higher temperatures such as 37 degrees C or 40 degrees C. This strain could produce a pellicle polysaccharide in a shaking submerged culture as well as under static culture conditions. The polysaccharide was found to be attached to the bacterial cells. Although the polysaccharide production was higher at 30 degrees C than at 37 degrees C in shaking submerged culture, the productivity in static culture was not decreased even at higher temperatures. The membrane-attached polysaccharide was purified from the SKU 1100 strain by cell disruptions using either ultrasonic treatment or lysozyme treatment, followed by ultracentrifugation, enzyme treatments, dialysis against SDS, DEAE-cellulose column chromatography, alcohol precipitation, and gel filtration chromatography. The polysaccharide purified by the sonic treatment and also by the mild conditions using lysozyme treatment had the same average molecular mass of 120 kDa. The purified polysaccharide was composed of three different monosaccharides; glucose, galactose, and rhamnose, in an approximately equimolar ratio of 1:1:1.
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: Somporn
authors.initials: S
authors.lastname: Moonmangmee
authors.forename: Hirohide
authors.initials: H
authors.lastname: Toyama
authors.forename: Osao
authors.initials: O
authors.lastname: Adachi
authors.forename: Gunjana
authors.initials: G
authors.lastname: Theeragool
authors.forename: Napha
authors.initials: N
authors.lastname: Lotong
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushit
mesh_terms: D000091:Acetobacter / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry; D005638:Fruit / Q000382:microbiology; D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D009005:Monosaccharides / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D013104:Spheroplasts / Q000737:chemistry; D013785:Thailand
chemical_list: D009005:Monosaccharides; D011135:Polysaccharides, Bacterial