title: Effect of kLa on the production of glucose 6-phosphate dehydrogenase from Saccharomyces cerevisiae grown by fermentation process.
abstract: In a 5-L fermentor (NBS-MF 105), Saccharomyces cerevisiae (0.7 g/L) was inoculated into a liquid medium (pH 4.0) containing 17 g/L of glucose, 2.55 g/L of yeast extract, 4.25 g/L of peptone, 2.04 g/L of Na2HPO4 x 12H2O, 4.34 g/L of (NH4)2SO4 and 0.064 g/L of MgSO4 x 7H2O and aerobically cultivated at 35 degrees C for 22 h. Agitation and aeration were adjusted to attain initial kLa values of 15, 60, 135, and 230 h(-1). The glucose 6-phosphate dehydrogenase (G6PDH) productivity (PrG6PDH) obtained for kLa values of 15, 60, 135, and 230 h(-1) was 10.6, 31.8, 30.3, and 23.3 U/([Lx h]), respectively, whereas the cell productivity (Pr(x)) for the same kLa values were 0.24, 0.69, 0.69, and 0.49 g/[L x h], respectively. Thus, both events are coupled and depend on the dissolved oxygen in the medium.
authors.affiliation: Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmacy, Univeristy of São Paulo, SP, Brazil.
authors.forename: Daniel Pereira
authors.initials: DP
authors.lastname: Silva
authors.forename: Adalberto
authors.initials: A
authors.lastname: Pessoa
authors.forename: Inês C
authors.initials: IC
authors.lastname: Roberto
authors.forename: Michele
authors.initials: M
authors.lastname: Vitolo
mesh_terms: D005285:Fermentation; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D008962:Models, Theoretical; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development*
chemical_list: D005954:Glucosephosphate Dehydrogenase