Kidney preservation in pigs with University of Wisconsin and Celsior solution.
BACKGROUND
Although University of Wisconsin (UW) solution continues to be the most commonly used for intra-abdominal organs a new solution, Celsior, already utilized for heart and lungs, has been proposed for kidney and liver preservation. The aim of this research was to assess the effect of Celsior compared to UW on the immediate graft function of pig autotransplanted kidneys.


METHODS
We used an autotransplantation pig model to avoid immunological interferences. After harvesting the graft was immediately bench perfused through the renal artery with 200 cc of solution and preserved for 24, 32 and 48 hrs. After cold ischemia grafts were autotransplanted on cava vein and aorta and a ureteral-ureteral anastomosis was performed. Contralateral nephrectomy was carried out at the end of this procedure. Each experimental group was composed of 5 animals. All animals were sacrificed after 1 week.


RESULTS
All animals with 24 hr and 32 hr preserved grafts survived up to sacrifice; kidney function was recovered in all animals of 24 hr groups and in most of the animals of 32 hr preserved grafts (4/5) without any differences between the 2 solutions. In Celsior and UW 48 hr groups only 5 animals survived (3 and 2, respectively) and in all these animals kidney function was not totally recovered.


CONCLUSIONS
Our data show that preservation of kidneys with the Celsior solution in the experimental setting is equivalent to UW solution.
Cavallari
G
G
Department of Surgery and Transplantation, University of Bologna, S. Orsola Hospital, Bologna, Italy.
Catena
F
F
Santoni
B
B
Montalti
R
R
Turi
P
P
Beltempo
P
P
Bertelli
R
R
Piazzese
E
E
Pacilè
V
V
Faenza
A
A
Nardo
B
B
Cavallari
A
A
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D005978:Glutathione; D006639:Histidine; D007328:Insulin; D016030:Kidney Transplantation; D008353:Mannitol; D019188:Organ Preservation Solutions; D011887:Raffinose; D013552:Swine; D014182:Transplantation, Autologous
C088453:Celsior; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D006639:Histidine; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose