Expression of HSP70 gene in rat brain after exposures to repeated +Gz.
OBJECTIVE
To study the changes of mRNA expression of heat shock protein 70 (HSP70) in the rat brain exposed to repeated +Gz.


METHODS
The mRNA expression levels of HSP70 in rat brain were measured by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
The HSP70 mRNA expression levels in rat brains taken 30 min and 6 h after repeated +Gz exposures were significantly higher than those in control group, while the difference between the levels of control group and those of experimental rat brains taken 24 h after +Gz exposure was not significant.


CONCLUSIONS
It is suggested that HSP70 mRNA expression in rat brain can be induced by repeated +Gz exposures and the increased HSP70 mRNA expression may play an important role in self-protection against brain damage induced by +Gz exposures.
Cai
Q
Q
Molecular Biology Research Center, General Hospital of The Air Force, Beijing.
Liu
H J
HJ
Chen
Y C
YC
Ji
G Y
GY
Chen
T X
TX
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000502:physiology; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018471:Hypergravity; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger