Intranasal immunization with recombinant antigens associated with new cationic particles induces strong mucosal as well as systemic antibody and CTL responses.
New cationic nanoparticles (SMBV) were evaluated for use as a nasal vaccine delivery system for two recombinant proteins: HBsAg and beta-galactosidase. Each protein was formulated with SMBV and intranasally administrated to non-anesthetized mice. In each model, the formulated protein induced high levels of specific serum IgG antibodies and cytotoxic T lymphocyte (CTL) responses. Moreover, specific IgA antibodies were found in nasal as well as in vaginal washes of intranasally immunized mice with the protein associated with SMBV. In contrast, no IgG or IgA antibodies and no CTL were detected in mice immunized with free protein. The detection of a CTL response and an increase in both IgG1 and IgG2a antibodies in serum suggest that SMBV amplifies both Th1 and Th2 responses without modifying the Th1/Th2 profile of the immune response induced by the natural protein. These data demonstrate the high potential of SMBV for use as a nasal delivery system for sub-unit vaccines.
Debin
Arnaud
A
Biovector Therapeutics, Chemin du Chêne Vert, BP 169, 31676 Labège, France.
Kravtzoff
Roger
R
Santiago
Jocelyn Vaz
JV
Cazales
Laurence
L
Sperandio
Sandrine
S
Melber
Karl
K
Janowicz
Zbigniew
Z
Betbeder
Didier
D
Moynier
Marinette
M
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology; D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000917:Antibody Formation; D002412:Cations / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D007111:Immunity, Cellular; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000276:immunology; D001616:beta-Galactosidase / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D002412:Cations; D006514:Hepatitis B Surface Antigens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014614:Vaccines, Synthetic; D001616:beta-Galactosidase