Studies on mass production of transformed Panax ginseng hairy roots in bioreactor.
The growth properties of Panax ginseng hairy roots transformed by Agrobacterium rhizogenes were compared between flask and aerated column or stirred bioreactor. In flask cultures, sucrose, initially 30 g/L, was nearly exhausted after 45 d of culture. The pH of the medium dropped from 5.5 to 4.96 after 10 d, but afterward it gradually increased to 6.4. After 45 d, hairy roots grew about 16-folds. The growth rate of hairy roots in air-bubble column or stirred bioreactor cultures was 1.13 (1.11) to 1.23 (1.20) g fresh wt (dry wt)/(g of cells x d), respectively. For both bioreactors, growth was about three times as high as in the flask cultivation.
Jeong
Gwi-Taek
GT
Faculty of Chemical Engineering, Chonnam National University, Kwangju, Korea.
Park
Don-Hee
DH
Hwang
Baik
B
Park
Kyungmoon
K
Kim
Si-Wouk
SW
Woo
Je-Chang
JC
D000388:Air; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D002478:Cells, Cultured; D007700:Kinetics; D005894:Panax / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D012231:Rhizobium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012503:Saponins / Q000378:metabolism*; D013997:Time Factors
D012503:Saponins