An Arabidopsis thaliana pectin acetylesterase gene is upregulated in nematode feeding sites induced by root-knot and cyst nematodes.
By using differential display, gene expression was investigated in Arabidopsis thaliana roots shortly after nematode infection, and a putative pectin acetylesterase (PAE) homolog (DiDi 9C-12) was found to be upregulated. PAEs catalyze the deacetylation of pectin, a major compound of primary cell walls. mRNA in situ hybridization experiments showed that the expression of DiDi 9C-12 was enhanced very early after infection in initiating giant-cells and in cells surrounding the nematodes. Later on, the level of DiDi 9C-12 mRNA was lower in giant-cells and transcripts were mainly found in parenchyma, endodermis, and pericycle cells of the root gall. Twenty days after infection, DiDi 9C-12 transcripts could no longer be detected. DiDi 9C-12 transcripts were also found in young syncytia and in the cells surrounding the expanding syncytium. Our results suggest that plant parasitic nematodes can modulate the rapid growth of the feeding cells and the expansion of the root gall by triggering the expression of DiDi 9C-12. PAEs, which probably act together with a range of other pectin-degrading enzymes, could be involved in softening and loosening the primary cell wall in nematode-infected plant roots.
Vercauteren
Isabel
I
Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, Belgium.
de Almeida Engler
Janice
J
De Groodt
Ruth
R
Gheysen
Godelieve
G
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000469:parasitology; D029681:Arabidopsis Proteins / Q000235:genetics*; D004950:Esterases / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009348:Nematoda / Q000254:growth & development*; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000469:parasitology; D017386:Sequence Homology, Amino Acid; D014431:Tylenchoidea / Q000254:growth & development; D015854:Up-Regulation / Q000235:genetics
D029681:Arabidopsis Proteins; D004950:Esterases; C100533:pectinacetylesterase