Synthesis of a novel class of polyhydroxyalkanoates in Arabidopsis peroxisomes, and their use in monitoring short-chain-length intermediates of beta-oxidation.
The poly[(R)-3-hydroxyalkanoate] (PHA) synthase gene (phaC(Ac)) of Aeromonas caviae FA440 was modified by adding a peroxisome targeting signal encoding the last 10 amino acids at the carboxyl-terminal of spinach glycolate oxidase. The modified gene was introduced into Arabidopsis thaliana plants by Agrobacterium-mediated transformation. The transgenic Arabidopsis plant expressed the introduced gene and its protein, and it accumulated PHA in its tissues. Gas chromatography-mass spectrometry analysis demonstrated the accumulation of a novel type of PHA, poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate). This strongly suggests that short-chain-length (R)-3-hydroxyacyl-CoAs were generated from intermediates of peroxisomal beta-oxidation. It was revealed by using this transgenic plant that Tween-20 can activate peroxisomal beta-oxidation of short-chain-length fatty acids.
Arai
Yuko
Y
RIKEN Institute, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198 Japan.
Nakashita
Hideo
H
Suzuki
Yoshikatu
Y
Kobayashi
Yumiko
Y
Shimizu
Toshiyuki
T
Yasuda
Michiko
M
Doi
Yoshiharu
Y
Yamaguchi
Isamu
I
D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000333:Aeromonas / Q000201:enzymology / Q000235:genetics; D016960:Agrobacterium tumefaciens / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D010084:Oxidation-Reduction / Q000187:drug effects; D020675:Peroxisomes / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011108:Polymers / Q000378:metabolism*; D011136:Polysorbates / Q000494:pharmacology; D012639:Seeds / Q000235:genetics; D000094122:Polyhydroxybutyrates
D000217:Acyltransferases; D005227:Fatty Acids; D011108:Polymers; D011136:Polysorbates; C435024:poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C077225:poly(3-hydroxyalkanoic acid) synthase