Comparison of SPA, FRET, and FP for kinase assays.
Wu
Jinzi J
JJ
Immunex Corporation, Seattle, WA, USA.
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D015195:Drug Design; D004353:Drug Evaluation, Preclinical; D005454:Fluorescence Polarization / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D009842:Oligopeptides / Q000737:chemistry; D011493:Protein Kinase C / Q000032:analysis; D011494:Protein Kinases / Q000032:analysis* / Q000276:immunology; D017346:Protein Serine-Threonine Kinases / Q000032:analysis; D011505:Protein-Tyrosine Kinases / Q000032:analysis; D012588:Scintillation Counting; D013379:Substrate Specificity
D000906:Antibodies; D009842:Oligopeptides; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D011493:Protein Kinase C