title: Cyclin E and chromosome instability in colorectal cancer cell lines.
abstract: OBJECTIVE
  The development of colorectal cancer depends on at least two distinct pathways involving genetic instability, namely: chromosome instability (CIN) and microsatellite instability. Cyclin E is involved in aneuploidy and several cancer types show an abnormal number of chromosomes.
  
  
  METHODS
  Cyclin E protein and mRNA values were analysed in human fetal skin fibroblasts and five colorectal cancer cell lines.
  
  
  RESULTS
  Cells with an aberrant number of chromosomes had higher cyclin E mRNA values and a significant increase in protein concentrations.
  
  
  CONCLUSIONS
  These data suggest that cyclin E regulation is altered in aneuploid cells and is an important factor in the CIN pathway.
authors.affiliation: Department of Internal and Public Medicine, Division of Medical Genetics, University of Bari, Bari 70124, Italy. cristianosimone@hotmail.com
authors.forename: C
authors.initials: C
authors.lastname: Simone
authors.forename: N
authors.initials: N
authors.lastname: Resta
authors.forename: L
authors.initials: L
authors.lastname: Bagella
authors.forename: A
authors.initials: A
authors.lastname: Giordano
authors.forename: G
authors.initials: G
authors.lastname: Guanti
mesh_terms: D002869:Chromosome Aberrations; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D019927:Cyclin E / Q000235:genetics* / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D019927:Cyclin E; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm