Antisense c-erbB-2 Decreases DNA Repair and Apoptosis Inducibility in Human Diploid Fibroblasts.
pDOR-erbB-2 sense and antisense retroviral expression vectors were introduced into normal human diploid fibroblasts(2BS), respectively, to construct 2BS-A cells(transfected with antisense recombinant vector) and 2BS-S cells (transfected with sense recombinant vector). Southern blot analysis verified that the exgenous c-erbB-2 cDNA were integrated into genomic DNA. Compared with the cells transfected with pDOR-neo empty vector, 2BS-A cells exhibited decreased ability not only to repair DNA damage, but also to undergo apoptosis. This was consistent with our observations that senescent phenotype was earlier appeared in these cells.
Zhang
Mao
M
Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100083, China. ttjzzy@public.gb.com.cn
Zhang
Zong-Yu
ZY
Tong
Tan-Jun
TJ