Biodegradation of cis-dichloroethene as the sole carbon source by a beta-proteobacterium.
An aerobic bacterium capable of growth on cis-dichloroethene (cDCE) as a sole carbon and energy source was isolated by enrichment culture. The 16S ribosomal DNA sequence of the isolate (strain JS666) had 97.9% identity to the sequence from Polaromonas vacuolata, indicating that the isolate was a beta-proteobacterium. At 20 degrees C, strain JS666 grew on cDCE with a minimum doubling time of 73 +/- 7 h and a growth yield of 6.1 g of protein/mol of cDCE. Chloride analysis indicated that complete dechlorination of cDCE occurred during growth. The half-velocity constant for cDCE transformation was 1.6 +/- 0.2 microM, and the maximum specific substrate utilization rate ranged from 12.6 to 16.8 nmol/min/mg of protein. Resting cells grown on cDCE could transform cDCE, ethene, vinyl chloride, trans-dichloroethene, trichloroethene, and 1,2-dichloroethane. Epoxyethane was produced from ethene by cDCE-grown cells, suggesting that an epoxidation reaction is the first step in cDCE degradation.
Coleman
Nicholas V
NV
Air Force Research Laboratory-MLQL, Tyndall AFB, Florida 32403, USA.
Mattes
Timothy E
TE
Gossett
James M
JM
Spain
Jim C
JC
D001673:Biodegradation, Environmental; D004000:Dichloroethylenes / Q000378:metabolism*; D020560:Proteobacteria / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D013816:Thermodynamics
D004000:Dichloroethylenes