What is the best sample for determining the early postmortem period by on-the-spot flow cytometry analysis?
The level of degradation of DNA as a means for determining the time of death has been proposed as a valid adjunct to the classic thanatochronologic methods. The twofold aim of this work was to determine which organ might reveal both a correlation between the percentage of degradation of the DNA and the time lapse since death, and would be easiest to sample and yield the most reproducible results even in technically unfavorable situations such as on-the-spot investigations at the scene of death. A comparison of the spleen, blood, and liver showed that hepatic tissue best meets these specific needs because it shows a virtually linear correlation between the time elapsed since death and the level of degradation of the DNA, and it can easily be sampled at the scene of death by use of a common biopsy needle.
Di Nunno
Nunzio
N
Section of Forensic Medicine, Department of Internal Medicine and Public Medicine, University of Bari, Italy. forem@medicinalegale.uniba.it
Costantinides
Fulvio
F
Cina
Stephen J
SJ
Rizzardi
Clara
C
Di Nunno
Cosimo
C
Melato
Mauro
M
D001344:Autopsy / Q000379:methods; D001773:Blood Cells / Q000473:pathology; D004247:DNA / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008099:Liver / Q000473:pathology; D011180:Postmortem Changes; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods; D013154:Spleen / Q000473:pathology; D013997:Time Factors
D004247:DNA