Soybean cell enlargement oscillates with a temperature-compensated period length of ca. 24 min.
Rate of enlargement of epidermal cells from soybean, when measured at intervals of 1 min using a light microscope equipped with a video measurement system, oscillated with a period length of about 24 min. This oscillation parallels the 24-min periodicity observed for the oxidation of NADH by the external plasma membrane NADH oxidase. The increase in length was not only non-linear, but intervals of rapid increase in area alternated with intervals of rapid decrease in area. The length of the period was temperature compensated, and was approximately the same when measured at 14, 24 and 34 degrees C even though the rate of cell enlargement varied over this same range of temperatures. These observations represent the first demonstration of an oscillatory growth behavior correlated with a biochemical activity where the period length of both is independent of temperature (temperature compensated) as is the hallmark of clock-related biological phenomena.
Morre
D J
DJ
Department of Medicinal Chemistry, Purdue University, West Lafayette, IN 47907-1333, USA. Morre@pharmacy.purdue.edu.
Pogue
R
R
Morre
D M
DM
D048429:Cell Size / Q000502:physiology; D018546:Hypocotyl / Q000166:cytology / Q000201:enzymology; D018715:Microscopy, Video; D009097:Multienzyme Complexes / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010507:Periodicity; D019441:Plant Epidermis / Q000166:cytology* / Q000201:enzymology; D013025:Glycine max / Q000166:cytology* / Q000201:enzymology*; D013696:Temperature
D009097:Multienzyme Complexes; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases