Excision of selectable marker genes from transgenic plants.
Selectable marker genes are required to ensure the efficient genetic modification of crops. Economic incentives and safety concerns have prompted the development of several strategies (site-specific recombination, homologous recombination, transposition, and co-transformation) to eliminate these genes from the genome after they have fulfilled their purpose. Recently, chemically inducible site-specific recombinase systems have emerged as valuable tools for efficiently regulating the excision of transgenes when their expression is no longer required. The implementation of these strategies in crops and their further improvement will help to expedite widespread public acceptance of agricultural biotechnology
Hare
Peter D
PD
Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Chua
Nam-Hai
NH
D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D017353:Gene Deletion; D018506:Gene Expression Regulation, Plant; D018014:Gene Transfer Techniques; D005818:Genetic Engineering / Q000379:methods; D005819:Genetic Markers; D030821:Plants, Genetically Modified / Q000145:classification / Q000235:genetics*; D045522:Recombinases; D011995:Recombination, Genetic; D019076:Transgenes / Q000235:genetics*
D004251:DNA Transposable Elements; D005819:Genetic Markers; D045522:Recombinases; D004254:DNA Nucleotidyltransferases