title: HSP70 and HSP90 expression in leucocytes after exercise in moderately trained humans.
abstract: In this study, we examined expression of heat shock proteins (HSP) 70 and 90 in human leucocytes after moderate-to-heavy exercise. We also compared baseline levels of HSP70 and HSP90 in trained (TR) and untrained (UT) subjects. Eleven TR subjects ran on a treadmill for 1 h at 70% of maximal oxygen consumption. The HSP levels were measured prior to exercise and 15 and 24 h after exercise. Baseline HSP levels were also measured in eight UT controls. Fifteen hours and 24 h after exercise, TR subjects showed no significant increases in HSP70 (P > 0.05). The HSP90 levels also did not change (P > 0.05). Baseline HSP70 levels in TR subjects were lower than in UT subjects (2.04 +/- 0.51 ng vs. 4.52 +/- 0.95 ng, P < 0.05), while HSP90 levels were similar in TR and UT subjects. We conclude that exercise at an intensity that is within normal limits for a moderately trained individual is not a sufficient stimulus of HSP70 production in leucocytes. We also conclude that blunted levels of baseline HSP70 expression in TR subjects might be a chronic adaptation to training.
authors.affiliation: Department of Physiology, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
authors.forename: S
authors.initials: S
authors.lastname: Shastry
authors.forename: D O
authors.initials: DO
authors.lastname: Toft
authors.forename: M J
authors.initials: MJ
authors.lastname: Joyner
mesh_terms: D000222:Adaptation, Physiological; D000328:Adult; D015153:Blotting, Western; D001823:Body Composition; D001835:Body Weight; D015536:Down-Regulation; D004591:Electrophoresis, Polyacrylamide Gel; D015444:Exercise / Q000502:physiology*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D010101:Oxygen Consumption
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins