title: Analysis of receptor (CD46, CD150) usage by measles virus.
abstract: In order to investigate which measles virus (MV)-strains use CD46 and/or CD150 (signalling lymphocytic activation molecule, SLAM) as receptors, CHO cells expressing either recombinant CD46 or SLAM were infected with a panel of 28 MV-strains including vaccine strains, wild-type strains with various passage histories and recombinant viruses. We found that SLAM served as a common receptor conferring virus uptake and syncytium formation for all MV-strains tested. Predominantly vaccine and laboratory adapted strains, but also a minor fraction of the wild-type strains tested, could utilize both CD46 and SLAM. Using recombinant viruses, we demonstrate that the single amino acid exchange in the haemagglutinin (H) protein at position 481 Asn/Tyr (H481NY) determines whether the virus can utilize CD46. This amino acid alteration has no affect on the usage of SLAM as receptor, and as such demonstrates that the binding sites for SLAM and CD46 are distinct.
authors.affiliation: Institut für Virologie und Immunbiologie, Versbacher Str. 7, D-97078 Würzburg, Germany1.
authors.forename: Christian
authors.initials: C
authors.lastname: Erlenhöfer
authors.affiliation: School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT9 7BL, UK2.
authors.forename: W Paul
authors.initials: WP
authors.lastname: Duprex
authors.affiliation: School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT9 7BL, UK2.
authors.forename: Bert K
authors.initials: BK
authors.lastname: Rima
authors.affiliation: Institut für Virologie und Immunbiologie, Versbacher Str. 7, D-97078 Würzburg, Germany1.
authors.forename: Volker
authors.initials: V
authors.lastname: Ter Meulen
authors.affiliation: Institut für Virologie und Immunbiologie, Versbacher Str. 7, D-97078 Würzburg, Germany1.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schneider-Schaulies
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000378:metabolism*; D001216:Asparagine; D016466:CHO Cells; D006224:Cricetinae; D015726:Giant Cells; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism*; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis / Q000378:metabolism*; D008459:Measles virus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism*; D011956:Receptors, Cell Surface; D011991:Receptors, Virus / Q000032:analysis / Q000378:metabolism*; D011995:Recombination, Genetic; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014443:Tyrosine; D014779:Virus Replication
chemical_list: D015703:Antigens, CD; C497857:CD46 protein, human; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D007136:Immunoglobulins; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C000604059:SLAMF1 protein, human; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014443:Tyrosine; D001216:Asparagine