title: Human recombinant EGF protein delivered by a biodegradable cell transplantation system.
abstract: We have previously shown a new approach to expand cultured human keratinocytes and reconstitute the epidermis in full-thickness wounds using a new microsperical transport system. This was a new approach to increase the cell yield for seeding without altering the anchoring proteins by enzymatic steps. That time we used Cytodex 3 which failed to be degraded and induced an inflammatory reaction in a t-cell-deficient organism. Therefore, we have investigated another microcarrier consisting of PLGA, which is a well-known carrier material for cell culture and transplantation. After coating the PLGA carrier with gelatine the seeding time of viable cells reached 4 h and the cell gain after 7 days of spinner culture was 16-fold. At 14 days after transplantation, we could detect a new stratified epithelium in our full-thickness wound healing model. Because cytokines play a major role in wound healing, we loaded this carrier material with different concentrations of rhEGF, showing a dose dependent release of the protein in vitro and in vivo. This result might lead to a different approach in the treatment of wounds.
authors.affiliation: Department of Plastic and Hand Surgery, University of Freiburg Medical Center, Germany. voigt@ch11.ukl.uni-freiburg.de
authors.forename: M
authors.initials: M
authors.lastname: Voigt
authors.forename: C
authors.initials: C
authors.lastname: Andree
authors.forename: T
authors.initials: T
authors.lastname: Kalt
authors.forename: S
authors.initials: S
authors.lastname: Dörmann
authors.forename: D J
authors.initials: DJ
authors.lastname: Schaefer
authors.forename: K-J
authors.initials: KJ
authors.lastname: Walgenbach
authors.forename: G B
authors.initials: GB
authors.lastname: Stark
mesh_terms: D020341:Absorbable Implants; D000328:Adult; D000818:Animals; D001672:Biocompatible Materials; D017690:Cell Transplantation / Q000379:methods*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004815:Epidermal Growth Factor / Q000008:administration & dosage*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects*; D019344:Lactic Acid; D051379:Mice; D008819:Mice, Nude; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D011994:Recombinant Proteins / Q000008:administration & dosage; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D011108:Polymers; D011994:Recombinant Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004815:Epidermal Growth Factor