Molecular modeling studies of the DCCD-treated cytochrome bc1 complex: predicted conformational changes and inhibition of proton translocation.
Dicyclohexylcarbodiimide (DCCD) binds covalently to an acidic amino acid located in the cd loop connecting membrane-spanning helices C and D of cytochrome b resulting in an inhibition of proton translocation in the cytochrome bc1 complex with minimal effects on the steady state rate of electron transfer. Single turnover studies performed with the yeast cytochrome bc1 complex indicated that the initial phase of cytochrome b reduction was inhibited 25-45% in the DCCD-treated cytochrome bc1 complex, while the rate of cytochrome c1 reduction was unaffected. Simulations by molecular modeling predict that binding of DCCD to glutamate 163 located in the cd2 loop of cytochrome b of chicken liver mitochondria results in major conformational changes in the protein. The conformation of the cd loop and the end of helix C appeared twisted with a concomitant rearrangement of the amino acid residues of both cd1 and cd2 loops. The predicted rearrangement of the amino acid residues of the cd loop results in disruptions of the hydrogen bonds predicted to form between amino acid residues of the cd and ef loops. Simultaneously, two new hydrogen bonds are predicted to form between glutamate 272 and two residues, aspartate 253 and tyrosine 272. Formation of these new hydrogen bonds would restrict the rotation and protonation of glutamate 272, which may be necessary for the release of the second electrogenic proton obtained during ubiquinol oxidation in the bc1 complex.
Wang
Yudong
Y
Department of Biochemistry and Molecular Pharmacology, School of Medicine, West Virginia University, Morgantown 26505-9142, USA.
Beattie
Diana S
DS
D001665:Binding Sites; D004024:Dicyclohexylcarbodiimide / Q000378:metabolism / Q000494:pharmacology*; D014450:Electron Transport Complex III / Q000737:chemistry* / Q000187:drug effects* / Q000378:metabolism; D018698:Glutamic Acid / Q000737:chemistry; D006860:Hydrogen Bonding; D007700:Kinetics; D008958:Models, Molecular; D010084:Oxidation-Reduction; D011487:Protein Conformation / Q000187:drug effects; D011522:Protons; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism
D011522:Protons; D029701:Saccharomyces cerevisiae Proteins; D018698:Glutamic Acid; D004024:Dicyclohexylcarbodiimide; D014450:Electron Transport Complex III