title: Orthotopic SCID mouse model with two newly established human lung squamous carcinoma cell lines.
abstract: The objective of this study was to present an orthotopic SCID mouse model using two newly established human cell lines derived from squamous cell carcinomas of the lung. GO-LCC1 cells (1 x 10(6)) and GO-LCC2 cells (3 x 10(6)) were implanted orthotopically into the right lungs of 10 C.B. 17 scid/scid mice. In both groups, aggressive tumor growth with partial infiltration of the thoracic wall, heart and diaphragm was observed. In only one case distant metastases in the contralateral lung were detected, while all animals developed tumors at the site of inoculation. Xenografted GO-LCC1 cells showed enhanced tumor propagation compared to GO-LCC2 cells. Our data underscore the usefulness of the SCID mouse model to study the biological behaviour of cultuved human tumor cells and furthemore demonstrate the clinical applicability of xenograft techniques using human tumor cell lines derived from pneumonectomy specimens of squamous lung carcinomas.
authors.affiliation: Department of Thoracic, Heart and Vascular Surgery, University of Göttingen, Germany. m.mirzaie@t-online.de
authors.forename: Masoud
authors.initials: M
authors.lastname: Mirzaie
authors.forename: Bastian
authors.initials: B
authors.lastname: Gunawan
authors.forename: Thomas
authors.initials: T
authors.lastname: Meyer
authors.forename: Bernhard
authors.initials: B
authors.lastname: Herse
authors.forename: László
authors.initials: L
authors.lastname: Füzesi
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Ruschewski
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured