Efficacy of immunization with ferric citrate receptor FecA from Escherichia coli on induced coliform mastitis.
The effects of immunization with the ferric citrate receptor FecA on antibody responses and on experimentally induced mastitis following intramammary challenge were investigated. Twenty-one cows were assigned to seven blocks of three cows based on expected parturition. Cows within block were randomly assigned to one of three treatments: 1) FecA immunization, 2) Escherichia coli J5 immunization, and 3) unimmunized controls. Challenge was by infusion of approximately 60 cfu of E. coli 727 into one uninfected mammary gland between 13 and 31 d after parturition. Cows within block were challenged on the same day. Cows immunized with FecA had higher immunoglobulin (Ig)G titers against FecA in serum and in mammary secretions at calving, immediately before challenge, and 7 d after challenge than did cows immunized with E. coli J5 or control cows. Immunization with FecA also increased IgG titers against whole-cell E. coli 727 in serum and in mammary secretions at calving. Serum IgM titers against FecA were higher in FecA immunized cows than in other treatment groups immediately before challenge. Bacterial counts in milk, duration of bacterial isolation in milk, rectal temperature, and milk somatic cell counts following intramammary challenge were similar among treatments. Milk production and dry matter intake did not differ among treatments. The ferric citrate receptor FecA was immunogenic in cows, but immunization had minimal effect on the clinical severity of experimentally induced E. coli mastitis.
Takemura
K
K
Department of Animal Sciences, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster 44691, USA.
Hogan
J S
JS
Lin
J
J
Smith
K L
KL
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology*; D002417:Cattle; D002452:Cell Count / Q000662:veterinary; D015169:Colony Count, Microbial / Q000662:veterinary; D004926:Escherichia coli / Q000254:growth & development / Q000276:immunology*; D004927:Escherichia coli Infections / Q000517:prevention & control / Q000662:veterinary*; D029968:Escherichia coli Proteins; D005260:Female; D007114:Immunization / Q000662:veterinary*; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007075:Immunoglobulin M / Q000032:analysis / Q000097:blood; D007774:Lactation; D008414:Mastitis, Bovine / Q000382:microbiology / Q000517:prevention & control*; D008892:Milk / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D011897:Random Allocation; D011956:Receptors, Cell Surface; D013997:Time Factors; D016896:Treatment Outcome
D001428:Bacterial Vaccines; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C028589:FecA protein, E coli; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011956:Receptors, Cell Surface