title: Sac I Restriction Fragment Length Polymorphism (RFLP) related to the human CST2 gene.
abstract: Restriction Fragment Length Polymorphism (RFLP) related to cystatin gene (CST) family was detected in the Japanese population by using restriction enzyme Sac I. A polymorphic site, located at 0.9 kb from the 3' end of the CST2 gene, revealed a two allele polymorphism with band sizes of 3.5 kb and 8.3 kb by hybridization with probe including exon 2 of the CST1 gene. The gene frequencies in the Japanese population were 0.326 for 3.5 kb allele and 0.674 for 8.3 kb allele (n = 86). The phenotypes of the polymorphism showed no association with the previously reported electrophoretic cystatin SA protein phenotypes.
authors.affiliation: Department of Forensic Odontology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Minaguchi
authors.forename: Tateshi
authors.initials: T
authors.lastname: Kiriyama
authors.forename: Eiichi
authors.initials: E
authors.lastname: Saitoh
authors.forename: Satoko
authors.initials: S
authors.lastname: Isemura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sanada
mesh_terms: D000483:Alleles; D015139:Blotting, Southern; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D015891:Cystatins / Q000235:genetics*; D005787:Gene Frequency; D006801:Humans; D007564:Japan; D005810:Multigene Family; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012471:Salivary Proteins and Peptides / Q000235:genetics*
chemical_list: D015891:Cystatins; D012471:Salivary Proteins and Peptides