[Modulation of voltage-gated sodium channels by recombinant interferon-alpha2b in the human neuroblastoma cell line IMR-32].
Clonal human neuroblastoma cells imr-32 are a suitable model system for studies of neuronal excitability modulation. The ability interferon-alpha 2b "laferon" to modulate the mechanisms of electrical activity was studied in whole-cell patch-clamped undifferentiated human neuroblastoma cells IMR-32. It was shown that 1 h incubation of IMR-32 cells at 37 degrees C in medium with laferon (600 U/ml) exerted changes in voltage-dependent properties of Na(+)-channels. The results of the present study demonstrate that laferon decreased of Na(+)-channels sensitivity to changes of membrane potential leading of IMR-32 cells electrical excitability decrease.
Kucher
V V
VV
Bogomolets Institute of Physiology, NAS of Ukraine, Kyiv.
Magura
I S
IS
Rozhmanova
O M
OM
Dolgaia
E V
EV
Pogorelaia
N Kh
NKh
D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D015640:Ion Channel Gating / Q000187:drug effects*; D009447:Neuroblastoma / Q000378:metabolism* / Q000503:physiopathology; D018408:Patch-Clamp Techniques; D011994:Recombinant Proteins; D015222:Sodium Channels / Q000187:drug effects* / Q000502:physiology; D014407:Tumor Cells, Cultured
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D015222:Sodium Channels