p53AIP1 regulates the mitochondrial apoptotic pathway.
We identified recently the p53AIP1 gene, a novel p53 target that mediates p53-dependent apoptosis. In the experiments reported here, ectopic expression of p53AIP1 induced down-regulation of mitochondrial DeltaPsim and release of cytochrome c from mitochondria in human cells. Immunoprecipitation and immunostaining experiments indicated interaction between p53AIP1 and bcl-2 proteins at mitochondria. Overexpression of bcl-2 blocked the down-regulation of mitochondrial DeltaPsim and the proapoptotic activity of p53AIP1. Our results implicate p53AIP1 as a pivotal mediator of the p53-dependent mitochondrial apoptotic pathway.
Matsuda
Koichi
K
Human Genome Center, Institute of Medical Science, University of Tokyo, 461 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Yoshida
Koji
K
Taya
Yoichi
Y
Nakamura
Kozo
K
Nakamura
Yusuke
Y
Arakawa
Hirofumi
H
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins; D019556:COS Cells; D003574:Cytochrome c Group / Q000378:metabolism / Q000502:physiology; D015536:Down-Regulation; D006801:Humans; D007425:Intracellular Membranes / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology*; D011506:Proteins / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000502:physiology; D014407:Tumor Cells, Cultured
D051017:Apoptosis Regulatory Proteins; D003574:Cytochrome c Group; C414742:P53AIP1 protein, human; D011506:Proteins; D019253:Proto-Oncogene Proteins c-bcl-2