title: Pneumonia due to Bordetella bronchiseptica in a cystic fibrosis patient: 16S rRNA sequencing for diagnosis confirmation.
abstract: Bordetella bronchiseptica was identified as an unusual etiologic agent of pulmonary recurrent exacerbations and pneumonia in a cystic fibrosis (CF) patient by utilizing a 16S rRNA molecular kit in our hospital's clinical laboratory. This method appears to be a useful approach for identifying new emerging CF pathogens when discrepancies exist between phenotypical tests.
authors.affiliation: Laboratoire de Bactériologie-Hygiène. Département de Pneumologie, Hôpital A. Calmette, CHRU de Lille, Lille, France. fwallet@chru-lille.fr
authors.forename: Frédéric
authors.initials: F
authors.lastname: Wallet
authors.forename: Thierry
authors.initials: T
authors.lastname: Perez
authors.forename: Sylvie
authors.initials: S
authors.lastname: Armand
authors.forename: Benoit
authors.initials: B
authors.lastname: Wallaert
authors.forename: René J
authors.initials: RJ
authors.lastname: Courcol
mesh_terms: D000328:Adult; D001885:Bordetella Infections / Q000382:microbiology; D016950:Bordetella bronchiseptica / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003550:Cystic Fibrosis / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D006801:Humans; D018410:Pneumonia, Bacterial / Q000175:diagnosis* / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S