No soluble common cytokine receptor gamma chain (gamma(c)) in activated human lymphocyte cultures-comparison with soluble IL-2Ralpha.
The presence of a soluble form of the common cytokine receptor gamma chain (gamma(c)) in cell free supernatants from unstimulated and 1-6 days PHA stimulated peripheral blood lymphocyte (PBL) cultures was analyzed using a sandwich ELISA. No naturally produced soluble gamma(c) could be detected in these cell free culture supernatants, although a sensitivity in the nanogram range was achieved for recombinant baculovirus expressed human soluble gamma(c) with this assay (detection limit 0.2 ng hIL-2 sRgamma). Analysis of the very same supernatants for soluble IL-2Ralpha demonstrated increased concentrations (up to 20.4 ng/ml) of this other IL-2R member. The membrane-associated form of the common cytokine receptor gamma chain was detected in cell lysates prepared from stimulated PBL at a concentration of 3.5 ng per 0.5 x 10(6) cells. Analysis of a small panel of serum samples from patients with different disorders verified that the soluble form of hIL-2Ralpha, but not hIL-2 sRgamma, can be detected, which thereby strongly suggests that the human soluble gamma(c) seems to be a valuable marker only for a limited number of clinical disorders.
Lundin
Karolina
K
Department of Biology, Abo Akademi University, Tykistokatu 6, Fin-20520, Turku, Finland.
Tuukkanen
Anna-Maria
AM
Jansson
Christian
C
Nordström
Tommy
T
Lindqvist
Christer
C
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit; D008214:Lymphocytes / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D018123:Receptors, Interleukin / Q000032:analysis*; D011994:Recombinant Proteins; D012995:Solubility
C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D018123:Receptors, Interleukin; D011994:Recombinant Proteins