TFIIH plays an essential role in RNA polymerase I transcription.
TFIIH is a multisubunit protein complex that plays an essential role in nucleotide excision repair and transcription of protein-coding genes. Here, we report that TFIIH is also required for ribosomal RNA synthesis in vivo and in vitro. In yeast, pre-rRNA synthesis is impaired in TFIIH ts strains. In a mouse, part of cellular TFIIH is localized within the nucleolus and is associated with subpopulations of both RNA polymerase I and the basal factor TIF-IB. Transcription systems lacking TFIIH are inactive and exogenous TFIIH restores transcriptional activity. TFIIH is required for productive but not abortive rDNA transcription, implying a postinitiation role in transcription. The results provide a molecular link between RNA polymerase I transcription and transcription-coupled repair of active ribosomal RNA genes.
Iben
Sebastian
S
Division of Molecular Biology of the Cell II, German Cancer Research Center, D-69120, Heidelberg, Germany.
Tschochner
Herbert
H
Bier
Mirko
M
Hoogstraten
Deborah
D
Hozák
Pavel
P
Egly
Jean Marc
JM
Grummt
Ingrid
I
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002466:Cell Nucleolus / Q000378:metabolism / Q000648:ultrastructure; D002474:Cell-Free System; D003001:Cloning, Molecular; D004265:DNA Helicases; D004275:DNA, Ribosomal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D011506:Proteins / Q000378:metabolism / Q000648:ultrastructure; D012318:RNA Polymerase I / Q000235:genetics / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000096:biosynthesis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000172:deficiency / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic; D051759:Xeroderma Pigmentosum Group D Protein; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D011506:Proteins; D012335:RNA, Ribosomal; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D019473:Transcription Factors, TFII; C072022:XPBC-ERCC-3 protein; D051758:Transcription Factor TFIIH; D012318:RNA Polymerase I; D004265:DNA Helicases; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human