title: Molecular subtyping methods for detection of Salmonella enterica serovar Oranienburg outbreaks.
abstract: This study involved 82 Salmonella enterica serovar Oranienburg isolates from patients with gastroenteritis and/or focal infections, healthy carriers, and cuttlefish chips which were epidemiologically linked to a major outbreak that had affected 1,505 people in Japan between 1998 and 1999. We concurrently investigated four different molecular subtyping methods using human salmonellosis-associated Salmonella serovars and their applicability in detection of serovar Oranienburg in an outbreak. Pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic sequence PCR (ERIC2-PCR), or 16S/23S rRNA ribotyping provided a high degree of interserovar discrimination for most of the serovars, with PFGE being the most discriminatory. For intraserovar typing of serovar Oranienburg, ERIC2-PCR was found to be the most sensitive. Native plasmid profiling, however, revealed nine different subgroups among epidemiologically and genetically related outbreak strains. Using these methods, a link was confirmed between food (cuttlefish chips) and patients in the serovar Oranienburg outbreak. This study underscores the limitations of chromosome-based and plasmid-based typing methods.
authors.affiliation: Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba 305-8575, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Kumao
authors.forename: William
authors.initials: W
authors.lastname: Ba-Thein
authors.forename: Hideo
authors.initials: H
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D005399:Fishes / Q000382:microbiology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D021521:Ribotyping; D012478:Salmonella Food Poisoning / Q000453:epidemiology* / Q000382:microbiology; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification