Evaluation of different carbon and nitrogen sources in production of rhamnolipids by a strain of Pseudomonas aeruginosa.
Culture conditions involving variations in carbon and nitrogen sources and different C:N ratios were examined with the aim of increasing productivity in the process of rhamnolipid synthesis by Pseudomonas aeruginosa. In addition to the differences in productivity, the use of different carbon sources resulted in several proportions related to the types of rhamnolipids synthesized (monorhamnolipids and dirhamnolipids). Furthermore, the variation in nutrients, mainly the nitrogen source, resulted in different amounts of virulence factors, as phenazines and extracellular proteins. The data point out a new concern in the choice of substrate to be used for rhamnolipid production by P. aeruginosa: toxic byproducts.
Santos
Alexandre S
AS
Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Brazil. letam@acd.ufrj.br
Sampaio
Ana Paula W
AP
Vasquez
Gina S
GS
Santa Anna
Lídia M
LM
Pereira
Nei
N
Freire
Denise M G
DM
D001431:Bacteriological Techniques / Q000379:methods; D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media; D000431:Ethanol / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006017:Glycolipids / Q000096:biosynthesis* / Q000302:isolation & purification; D007700:Kinetics; D009584:Nitrogen / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*; D013024:Soybean Oil / Q000378:metabolism; D013379:Substrate Specificity
D003470:Culture Media; D006017:Glycolipids; D000069463:Olive Oil; D010938:Plant Oils; C418382:rhamnolipid; D000431:Ethanol; D002244:Carbon; D013024:Soybean Oil; D009584:Nitrogen; D005990:Glycerol