The biological and structural similarity between lunularic acid and abscisic acid.
Lunularic acid (LA) inhibited not only the germination and the growth of cress and lettuce at 1 mM but also the gibberellic acid (GA3)-induced alpha-amylase induction in embryoless barley seeds at 120 microM, which was recognized as a specific activity of abscisic acid (ABA). Moreover LA and ABA equally inhibited the growth of Lunularia cruciata A18 strain callus at 40 and 120 microM. A computational analysis revealed that the stable conformers of LA could be superimposed on the stable ABA conformers. In addition, the antibody raised against the conjugate of C1-ABA-bovine serum albumin (ABA-BSA) reacted with LA-horse-radish peroxidase (LA-HRP) conjugate as well as ABA-HRP conjugate, apparently. These results can explain why LA has ABA-like activity in higher plants. Moreover the results suggest that LA and ABA bind to the same receptor in higher plants.
Yoshikawa
Hiromichi
H
Department of Environmental Chemistry, Kyushu Kyoritsu University, Kitakyushu, Japan. fortuna@kyukyo-u.ac.jp
Ichiki
Yayoi
Y
Sakakibara
Keiko Doi
KD
Tamura
Hiroto
H
Suiko
Masahito
M
D000040:Abscisic Acid / Q000737:chemistry* / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D001467:Hordeum / Q000201:enzymology; D007700:Kinetics; D029686:Lepidium / Q000187:drug effects*; D018545:Lactuca / Q000187:drug effects*; D008958:Models, Molecular; D008968:Molecular Conformation; D012459:Salicylates / Q000737:chemistry* / Q000494:pharmacology*; D012639:Seeds / Q000187:drug effects* / Q000201:enzymology; D013267:Stilbenes; D000516:alpha-Amylases / Q000096:biosynthesis
D012459:Salicylates; D013267:Stilbenes; C067452:lunularic acid; D000040:Abscisic Acid; D000516:alpha-Amylases