Antiserum raised against the Epstein-Barr virus BARF0 protein reacts to HLA-DR beta chain.
The role that Epstein-Barr virus plays in nasopharyngeal carcinoma and Burkitt's lymphoma has been under intense study for many years. With only a limited set of viral genes being expressed in these tumours it has been difficult to understand how the virus could cause/aid in the generation of the tumours. In 1997 a paper was published by Fries et al. [Fries et al. (1997) Identification of a novel protein encoded by the BamH1 A region of the Epstein-Barr virus. J Virol 71: 2765-2771.] in which a rabbit serum was generated and used to identify protein products (RK-BARF0) encoded from the BamH1 A region of EBV. In this paper we have isolated these proteins from two-dimensional gels and identified them, using mass spectrometry, as components of HLA DR.
Schroder
W
W
The Queensland Institute of Medical Research, Brisbane, Australia.
Kienzle
N
N
Bushell
G
G
Sculley
T
T
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003429:Cross Reactions; D015180:Electrophoresis, Gel, Two-Dimensional; D005189:False Positive Reactions; D006684:HLA-DR Antigens / Q000737:chemistry / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology*
D000914:Antibodies, Viral; C086495:BARF1 protein, Human herpesvirus 4; D006684:HLA-DR Antigens; D007106:Immune Sera; D008565:Membrane Proteins; D014764:Viral Proteins