title: Nondestructive quality control for microarray production.
abstract: The use of microarrays to monitor gene expression has become a standard research tool at both academic and industrial research institutions. Quality control of common printing defects during DNA deposition onto glass substrates is critical to maintaining data integrity and preventing the needless consumption of precious RNA, labeling reagents, and time. Here we demonstrate a nondestructive method for monitoring the quality of every spot on every chip of a microarray production run. We have identified many common manufacturing defects, while not perturbing the attachment of our oligonucleotide target to the substrate or altering further hybridization. This protocol is simple, fast, and inexpensive.
authors.affiliation: Transcriptional Profiling Group, Biogen Inc., Cambridge, MA 02142, USA. jeff_shearstone@biogen.com
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Shearstone
authors.forename: Norman E
authors.initials: NE
authors.lastname: Allaire
authors.forename: Michael E
authors.initials: ME
authors.lastname: Getman
authors.forename: Steven
authors.initials: S
authors.lastname: Perrin
mesh_terms: D002232:Carbocyanines; D005456:Fluorescent Dyes; D006825:Hybridomas; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards*; D011786:Quality Control; D013601:T-Lymphocytes
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C089187:cyanine dye 3