Evidence that DNase I hypersensitive site 5 of the human beta-globin locus control region functions as a chromosomal insulator in transgenic mice.
We have previously reported that DNase I hypersensitive site 5 (5'HS5) of the human beta-globin locus control region functions as a chromatin insulator in stable transfection assays. In this report we show that a 3.2 kb DNA fragment containing the entire 5'HS5 region can protect a position-sensitive (A)gamma-globin gene against position effects in transgenic mice. Bracketing is required for function of 5'HS5 as an insulator. The 5'HS5 insulator operates in adult as well as in embryonic murine erythroid cells. The insulator has no significant stimulatory effects of its own. These results indicate that 5'HS5 can function as a chromatin insulator in vivo.
Li
Qiliang
Q
Division of Medical Genetics, Box 357720, Department of Medicine, University of Washington Medical School, Seattle, WA 98195, USA.
Zhang
Miaohua
M
Han
Hemei
H
Rohde
Alex
A
Stamatoyannopoulos
George
G
D000375:Aging / Q000097:blood / Q000235:genetics; D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003587:Cytomegalovirus / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D003850:Deoxyribonuclease I / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005914:Globins / Q000235:genetics*; D006801:Humans; D019426:Integrases / Q000235:genetics; D019901:Locus Control Region / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019076:Transgenes / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D015017:Yolk Sac / Q000378:metabolism
D002843:Chromatin; D004274:DNA, Recombinant; D012333:RNA, Messenger; D014764:Viral Proteins; D005914:Globins; C045073:Cre recombinase; D019426:Integrases; D003850:Deoxyribonuclease I