title: Electrophoretic injection within microdevices.
abstract: The flexibility of the microfabricated format creates unique opportunities for study of the electrophoretic process. The present work utilizes digital images to capture the motion of DNA samples during pre-electrophoretic processes. A systematic study of DNA loading and strong sample stacking (sample concentration effects) was performed in order to analyze realistic DNA analysis conditions within microdevices. Using digital imaging and microscopy, DNA sample profiles within the injector were analyzed by deconvolving the geometrical intensity profile into different velocity groups. This analysis illustrates the evolution of molecular separation into distinct migrating populations within the injector itself. The present study performed DNA injections within microfabricated devices imposing run voltages between 85 and 850 V/cm. Data from 3 different offset lengths of a double-T cross-injector, 10 different applied voltages, and 2 different sample preparation protocols are presented.
authors.affiliation: New York Center for Biomedical Engineering, Department of Mechanical Engineering, The City College of the City University of New York, New York 10031, USA. vazquez@ccny.cuny.edu
authors.forename: Maribel
authors.initials: M
authors.lastname: Vazquez
authors.forename: Gareth
authors.initials: G
authors.lastname: McKinley
authors.forename: Luba
authors.initials: L
authors.lastname: Mitnik
authors.forename: Samantha
authors.initials: S
authors.lastname: Desmarais
authors.forename: Paul
authors.initials: P
authors.lastname: Matsudaira
authors.forename: Daniel
authors.initials: D
authors.lastname: Ehrlich
mesh_terms: D004247:DNA / Q000032:analysis*; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted; D008832:Microchemistry
chemical_list: D004247:DNA