Laser capture microdissection and microarray expression analysis of lung adenocarcinoma reveals tobacco smoking- and prognosis-related molecular profiles.
Recent expression profile analyses revealed that lung adenocarcinomas can be divided into several subgroups with diverse pathological features. Because cellular heterogeneity of tumors can confound these analyses, we used laser capture microdissection and microarray expression analysis to characterize the molecular profiles of lung adenocarcinomas. We found 45 genes delineating smokers and nonsmokers that were located at chromosomal loci frequently altered in non-small cell lung cancers, and 27 genes, which were differentially expressed between survivors and nonsurvivors 5 years after surgery. These results are consistent with the hypothesis that the abnormal expression of genes involved in maintaining the mitotic spindle checkpoint and genomic stability, e.g., hBUB3, hZW10, and APC2, contribute to the molecular pathogenesis and tumor progression of tobacco smoke-induced adenocarcinoma of the lung.
Miura
Koh
K
Laboratory of Human Carcinogenesis, Biometric Research Branch, Division of Cancer Treatment and Diagnosis National Cancer Institute, NIH, 37 Convent Drive, Bethesda, MD 20892, USA..
Bowman
Elise D
ED
Simon
Richard
R
Peng
Amy C
AC
Robles
Ana I
AI
Jones
Raymond T
RT
Katagiri
Toyomasa
T
He
Ping
P
Mizukami
Hiroki
H
Charboneau
Lu
L
Kikuchi
Takefumi
T
Liotta
Lance A
LA
Nakamura
Yusuke
Y
Harris
Curtis C
CC
D000230:Adenocarcinoma / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D000368:Aged; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D004210:Dissection / Q000379:methods; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007834:Lasers; D008175:Lung Neoplasms / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012737:Sex Factors; D012907:Smoking / Q000009:adverse effects / Q000235:genetics*