Quantitative detection of respiratory Chlamydia pneumoniae infection by real-time PCR.
Real-time PCR was evaluated as a quantitative diagnostic method for Chlamydia pneumoniae infection using different respiratory samples. Real-time PCR had efficiency equal to or better than that of nested touchdown PCR. This study confirmed sputum as the best sampling material to detect an ongoing C. pneumoniae infection.
Kuoppa
Yvonne
Y
Department of Virology, University of Umeå, Umeå, Sweden. Yvonne.Kuoppa@climi.umu.se
Boman
Jens
J
Scott
Lena
L
Kumlin
Urban
U
Eriksson
Iréne
I
Allard
Annika
A
D023521:Chlamydophila Infections / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology*; D010614:Pharynx / Q000382:microbiology*; D018410:Pneumonia, Bacterial / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000382:microbiology*; D013183:Sputum / Q000382:microbiology*
D003470:Culture Media; D004269:DNA, Bacterial