A novel alanine-insensitive D-serine transporter in rat brain synaptosomal membranes.
D-Serine is an endogenous modulator of brain N-methyl-D-aspartate receptors. This study investigates serine transport in brain synaptosomal fractions. Saturable, temperature-dependent uptake of both D- and L-[3H]serine was observed. Alanine was only partially effective in blocking transport, arguing against involvement of system ASC. Inhibitors of the systems A, L and GLY were also ineffective. Saturation studies suggested a submillimolar K(m) for both D- and L-serine. These data suggest the presence of a novel serine transport system in rodent synaptosomes.
Javitt
Daniel C
DC
Nathan Kline Institute for Psychiatric Research/NYU School of Medicine, 140 Old Orangeburg Road, Orangeburg, NY 10962, USA. javitt@nki.rfmh.org
Balla
Andrea
A
Sershen
Henry
H
D000409:Alanine / Q000494:pharmacology*; D026905:Amino Acid Transport Systems / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D012694:Serine / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism*; D013997:Time Factors
D026905:Amino Acid Transport Systems; D016194:Receptors, N-Methyl-D-Aspartate; D012694:Serine; D000409:Alanine