[Study on gene expression changes of arsenic sulfide treated NB4 cells].
OBJECTIVE
To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.


METHODS
Two cDNA probes were made from mRNA of untreated or arsenic sulfide treated NB4 cells. The cells were labelled with Cy3 or Cy5 fluorescence dyes individually, hybridized with cDNA microarray, and scanned for fluorescent intensity. The altered gene expression was screened through the analysis of difference in gene expression profile.


RESULTS
Thirty four genes related to apoptosis, cell cycle and others expressed different after the treatment of arsenic sulfide, 28/34 were up-regulated, 6/34 down-regulated.


CONCLUSIONS
ABC50, PNAS-2 and cyclin G(2) might take part in the process of NB4 cell apoptosis induced by arsenic sulfide.
Gu
Chunhong
C
Department of Hematology, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Chen
Fangyuan
F
Han
Jieying
J
Teng
Ye
Y
Shao
Nianxian
N
Ouyang
Renrong
R
D001152:Arsenicals / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D013440:Sulfides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D001152:Arsenicals; D013440:Sulfides; C045816:arsenic trisulfide