Nitric oxide and hydroxyl radicals initiate lipid peroxidation by NMDA receptor activation.
In this experiment, we used direct electron paramagnetic resonance (EPR) spectra to measure lipid peroxidation by hydroxyl radical (.OH), nitric oxide (.NO) and lipid radical (.L). NMDA-receptor associated lipid peroxidation is thought to act through .OH in induction of neurotoxicity. The origin of .OH generation was found to arise mainly from peroxynitrite anion produced from O(2)(-) and .NO rather than from Fenton's reaction. This study verified that .OH generation from interactive reactions between .NO and O(2)(-) initiates NMDA-induced lipid peroxidation of PC12 cells.
Hayashi
Yoshihito
Y
Department of Psychiatry, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889-1692, Japan.
Ueda
Yuto
Y
Nakajima
Akira
A
Yokoyama
Hidekatsu
H
Mitsuyama
Yoshio
Y
Ohya-Nishiguchi
Hiroaki
H
Kamada
Hitoshi
H
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004578:Electron Spin Resonance Spectroscopy; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D016202:N-Methylaspartate / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*
D018690:Excitatory Amino Acid Agonists; D016194:Receptors, N-Methyl-D-Aspartate; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D016202:N-Methylaspartate