title: EDTA-dependent pseudothrombocytopenia confirmed by supplementation of kanamycin; a case report.
abstract: EDTA-dependent pseudothrombocytopenia (PTCP) is the phenomenon of a spurious low platelet count due to EDTA-induced aggregation of platelets. Since the failure to recognize EDTA-dependent PTCP may result in incorrect diagnosis and inappropriate treatment, the recognition of this phenomenon is very important. We report an insidious case of EDTA-dependent PTCP confirmed by supplementation of kanamycin to anticoagulant in a 53-year-old women. Although sodium citrate and heparin usually prevented the aggregation of platelets in EDTA-dependent PTCP patients, these anticoagulants failed in preventing PTCP in our case. EDTA-dependent PTCP was confirmed by the findings that the clumping of platelets on microscopic evaluation was found in EDTA-anticoagulated blood samples, whereas thrombocytopenia and platelet aggregation were not revealed in the sample supplemented with kanamycin.
authors.affiliation: Department of Internal Medicine, Konkuk University College of Medicine, Seoul, Korea.
authors.forename: Hae Lyun
authors.initials: HL
authors.lastname: Ahn
authors.forename: Young Il
authors.initials: YI
authors.lastname: Jo
authors.forename: Young Suk
authors.initials: YS
authors.lastname: Choi
authors.forename: Jung Yeon
authors.initials: JY
authors.lastname: Lee
authors.forename: Hae Woon
authors.initials: HW
authors.lastname: Lee
authors.forename: Seong Ryul
authors.initials: SR
authors.lastname: Kim
authors.forename: Joon
authors.initials: J
authors.lastname: Sim
authors.forename: Weon
authors.initials: W
authors.lastname: Lee
authors.forename: Chun Jo
authors.initials: CJ
authors.lastname: Jin
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000925:Anticoagulants / Q000009:adverse effects* / Q000494:pharmacology; D004492:Edetic Acid / Q000009:adverse effects*; D005260:Female; D006801:Humans; D007612:Kanamycin / Q000494:pharmacology*; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000627:therapeutic use*; D010976:Platelet Count; D013921:Thrombocytopenia / Q000097:blood* / Q000139:chemically induced
chemical_list: D000900:Anti-Bacterial Agents; D000925:Anticoagulants; D010975:Platelet Aggregation Inhibitors; D007612:Kanamycin; D004492:Edetic Acid