The effect of composition of parenteral solution on the thermal resistance of Bacillus stearothermophilus and Bacillus subtilis spores.
Large-volume parenteral solutions were submitted to heat treatments after being inoculated with Bacillus stearothermophilus ATCC 7953 (Tr = 121 degrees C) and Bacillus subtilis ATCC 9372 (Tr = 104.5 degrees C) spores. The average decimal reduction time for B. stearothermophilus ranged from a D121 degrees C value of 1.31 to 3.14 min, in glucophysiologic and Ringer's solutions respectively. For B. subtilis, D104.5 degrees C value increased from 0.69 to 1.37 min, in Ringer's (pH=5.91) and 50% glucose (pH 3.05) solutions respectively. The z value ranged from 7.95 degrees C (20% mannitol solution) to 13.14 degrees C (50% glucose solution), corresponding to an activation energy (Ea) of 81.48 and 49.30 kcal/mol, respectively.
Penna
Thereza Christina Vessoni
TC
Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Science, University of São Paulo, SP, Brazil. tcvpenna@usp.br
Marques
Marcelo
M
Machoshvili
Irena A
IA
Ishii
Marina
M
D001412:Bacillus subtilis / Q000502:physiology*; D002151:Calorimetry; D001411:Geobacillus stearothermophilus / Q000502:physiology*; D006358:Hot Temperature; D007263:Infusions, Parenteral; D013171:Spores, Bacterial / Q000502:physiology