A novel root-specific gene, MIC-3, with increased expression in nematode-resistant cotton (Gossypium hirsutum L.) after root-knot nematode infection.
A full-length cDNA, MIC-3, has been identified from a lambda ZAPII cDNA library constructed from the mRNA of nematode-resistant cotton (Gossypium hirsutum L.) roots after infection with root-knot nematode (Meloidogyne incognita). The putative open reading frame of MIC-3 encoded a protein of 141 amino acids with a calculated molecular mass of 15.3 kDa. Seven alternative polyadenylation sites have been identified for the MIC-3 transcripts, and the major transcripts are the longest ones. The MIC-3 gene contains a single intron within its coding region and belongs to a novel, multi-gene family containing up to six members. Expression of MIC-3 is root localized and specifically enhanced in the nematode induced, immature galls of resistant cotton line M-249, suggesting that MIC-3 may play a critical role in the resistance response to root-knot nematode.
Zhang
Xiang-Dong
XD
Department of Biochemistry and Molecular Biology, Box 9650, Mississippi State University, Mississippi State, MS 39762, USA.
Callahan
Franklin E
FE
Jenkins
Johnie N
JN
Ma
Din-Pow
DP
Karaca
Mehmet
M
Saha
Sukumar
S
Creech
Roy G
RG
D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D015723:Gene Library; D003368:Gossypium / Q000235:genetics* / Q000469:parasitology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010935:Plant Diseases / Q000209:etiology; D018517:Plant Roots / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics*
D018829:Adhesins, Bacterial; D002352:Carrier Proteins; C421382:MIC3 protein, Toxoplasma gondii; D015800:Protozoan Proteins