Effects of adenine-nucleotides on the sequence-specificity of origin recognition complex-binding to DNA.
We examined effects of adenine-nucleotides on the sequence-specificity of origin recognition complex (ORC)-binding to DNA, using a gel electrophoretic mobility shift assay. The sequence-specific DNA binding of ORC was observed in the presence of ATP or ATP-gamma-S but not in the presence of adenosine 5'-diphosphate (ADP) or in the absence of any adenine-nucleotides. In contrast, the sequence-independent DNA binding of ORC was observed under any one of these conditions. These results suggest that ATP increases the sequence-specificity of ORC-binding to DNA. In relation to the requirement for incubation at high temperature and inhibition by cardiolipin, there was no significant difference between the sequence-specific and the sequence-independent DNA binding activities of ORC.
Takenaka
Hitomi
H
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Takahashi
Naoko
N
Lee
Jong-Ryul
JR
Makise
Masaki
M
Yamaguchi
Yoshihiro
Y
Tsuchiya
Tomofusa
T
Mizushima
Tohru
T
D000227:Adenine Nucleotides / Q000494:pharmacology*; D002460:Cell Line; D004247:DNA / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D004586:Electrophoresis; D009154:Mutation; D051738:Origin Recognition Complex; D011485:Protein Binding
D000227:Adenine Nucleotides; D004268:DNA-Binding Proteins; D051738:Origin Recognition Complex; D004247:DNA