title: Rotational-echo double resonance characterization of vancomycin binding sites in Staphylococcus aureus.
abstract: Solid-state NMR experiments with stable isotope-labeled Staphylococcus aureus have provided insight into the structure of the peptidoglycan binding site of a potent fluorobiphenyl derivative of chloroeremomycin (Eli Lilly LY329332). Rotational-echo double resonance (REDOR) NMR provided internuclear distances from the 19F of this glycopeptide antibiotic to natural-abundance 31P and to specific 13C and 15N labels biosynthetically incorporated into the bacteria from labeled alanine, glycine, or lysine in the growth medium. Results from experiments with intact late log phase bacteria and cell walls indicated homogeneous drug-peptidoglycan binding. Drug dimers were not detected in situ, and the hydrophobic fluorobiphenyl group of LY329332 did not insert into the bilayer membrane. A model of the binding site consistent with the REDOR results positions the vancomycin cleft around an un-cross-linked D-Ala-D-Ala peptide stem with the fluorobiphenyl moiety of the antibiotic near the base of a second, proximate stem in a locally ordered peptidoglycan matrix.
authors.affiliation: Department of Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63130, USA.
authors.forename: Sung Joon
authors.initials: SJ
authors.lastname: Kim
authors.forename: Lynette
authors.initials: L
authors.lastname: Cegelski
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Studelska
authors.forename: Robert D
authors.initials: RD
authors.lastname: O'Connor
authors.forename: Anil K
authors.initials: AK
authors.lastname: Mehta
authors.forename: Jacob
authors.initials: J
authors.lastname: Schaefer
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001665:Binding Sites; D002473:Cell Wall / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008956:Models, Chemical; D010457:Peptidoglycan / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000737:chemistry*; D014640:Vancomycin / Q000031:analogs & derivatives* / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; C459087:LY 329332; D010457:Peptidoglycan; C112142:chloroeremomycin; D014640:Vancomycin