title: Effects of adenine-nucleotides on the sequence-specificity of origin recognition complex-binding to DNA.
abstract: We examined effects of adenine-nucleotides on the sequence-specificity of origin recognition complex (ORC)-binding to DNA, using a gel electrophoretic mobility shift assay. The sequence-specific DNA binding of ORC was observed in the presence of ATP or ATP-gamma-S but not in the presence of adenosine 5'-diphosphate (ADP) or in the absence of any adenine-nucleotides. In contrast, the sequence-independent DNA binding of ORC was observed under any one of these conditions. These results suggest that ATP increases the sequence-specificity of ORC-binding to DNA. In relation to the requirement for incubation at high temperature and inhibition by cardiolipin, there was no significant difference between the sequence-specific and the sequence-independent DNA binding activities of ORC.
authors.affiliation: Faculty of Pharmaceutical Sciences, Okayama University, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Takenaka
authors.forename: Naoko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Jong-Ryul
authors.initials: JR
authors.lastname: Lee
authors.forename: Masaki
authors.initials: M
authors.lastname: Makise
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Tomofusa
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Tohru
authors.initials: T
authors.lastname: Mizushima
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology*; D002460:Cell Line; D004247:DNA / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D004586:Electrophoresis; D009154:Mutation; D051738:Origin Recognition Complex; D011485:Protein Binding
chemical_list: D000227:Adenine Nucleotides; D004268:DNA-Binding Proteins; D051738:Origin Recognition Complex; D004247:DNA