title: Characterization of the glycoprotein B gene from ruminant alphaherpesviruses.
abstract: The complete open reading frame and promoter region of the glycoprotein B (gB) gene has been identified and sequenced from five poorly characterized alphaherpesviruses of ruminants, bovine herpesvirus 5 (BHV-5), buffalo herpesvirus 1 (BuHV-1), cervine herpesvirus 1 (CerHV-1), rangiferine herpesvirus 1 (RanHV-1), and caprine herpesvirus 1 (CapHV-1). One of the two regions identified with considerable sequence and length variation is also target of the immune system, as two B cell epitopes have been identified in this location. Features shared with bovine herpesvirus 1 (BHV-1) gB include two broad hydrophobic regions, six N-glycosylation sites and ten conserved cysteine residues in the gB extracellular domain. Phylogenetic analysis showed that the studied ruminant alphaherpesviruses form, together with BHV-1, a consistent group within the alpha2 subgroup of the herpesviruses. BHV-5 and BuHV-1 are most closely related to BHV-1, followed by CerHV-1, RanHV-1 and more distantly by CapHV-1. A remarkable high degree of sequence similarity was observed between BuHV-1 and the neuropathogenic BHV-5.
authors.affiliation: Department of Chemistry and Biochemistry, University of Bern, Switzerland. carlos.ros@ibc.unibe.ch
authors.forename: Carlos
authors.initials: C
authors.lastname: Ros
authors.forename: Sándor
authors.initials: S
authors.lastname: Belák
mesh_terms: D018138:Alphaherpesvirinae / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002020:Buffaloes; D002417:Cattle; D004279:DNA, Viral / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012418:Ruminants / Q000821:virology*; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics*; D014764:Viral Proteins
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins