Sequence analysis for assessing potential allergenicity.
Sequence analysis plays an important role in assessing the potential allergenicity of proteins used in transgenic foods, particularly for proteins that have not previously been part of the food supply. Sequence comparisons are used to indicate potential unexpected cross reactivity to existing allergens and to assess the potential for developing new sensitivities. Although the concept of using sequence analysis is straightforward, implementing a bioinformatic analysis that is accurate and complete can be complex. Several factors need to be considered, including the design and content of the sequence database, the analysis strategy, and the criteria for evaluating the results.
Gendel
Steven M
SM
Biotechnology Studies Branch, National Center for Food Safety and Technology, Summit-Argo, Illinois 60501, USA. sgendel@cfsan.fda.gov
D000465:Algorithms; D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D019295:Computational Biology / Q000379:methods*; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis; D020407:Internet; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D000485:Allergens; D000939:Epitopes