title: Utility of Y-STR haplotype and mtDNA sequence in personal identification of human remains.
abstract: The utility of Y-STR haplotype and mtDNA sequence in personal identification from human remains is demonstrated. In five cases, severely damaged human remains were identified by Y-chromosomal short tandem repeat (STR) (Y-STR) haplotyping and mitochondrial DNA (mtDNA) sequencing. DNA was extracted from highly adipoceratous tissues using the phenol-chloroform method and polymerase chain reaction amplified for Y-STR haplotyping and sequencing of two hypervariable regions, HV1 and HV2, of the mtDNA. The authors also profiled the Y-STR haplotype and mtDNA sequence of a blood sample that was obtained from the presumptive brother of the decedent. Y-STR haplotyping and mtDNA sequencing results were compared with one another and with the Anderson sequence. It was concluded from these data that the lower part of the body (Case 1), the human head (Case 2), the upper part of the body (Case 3), and the right arm (Case 4) were from the same individual, whereas another lower part of a body (Case 5) was from a relative of the presumptive brother. A novel descriptive way of a statistical characteristic, gene or haplotype diversity, is shown.
authors.affiliation: Department of Legal Medicine, Nagoya City University Hospital, Kawasumi Mizuho-ku, 467-8601, Japan.
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Koyama
authors.forename: Mineo
authors.initials: M
authors.lastname: Iwasa
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Tsuchimochi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Maeno
authors.forename: Ichiro
authors.initials: I
authors.lastname: Isobe
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Masataka
authors.initials: M
authors.lastname: Nagao
mesh_terms: D000328:Adult; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D020080:Tandem Repeat Sequences / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial