title: Determination of the docetaxel vehicle, polysorbate 80, in patient samples by liquid chromatography-tandem mass spectrometry.
abstract: A new simple method was developed for the quantitative determination of the docetaxel (Taxotere) vehicle, polysorbate 80 (Tween 80), in human plasma. Calibration curves were constructed in the range of 1-100 microg/ml, using paclitaxel (0.01 mM) as internal standard, and were analyzed using a power fit with equal weighting. Sample pretreatment involved a one-step extraction with acetonitrile-n-butyl chloride (1:4, v/v). The analytes were separated on a Waters X-Terra MS column (50x2.1 mm I.D.) packed with 3.5-microm ODS material, and eluted with methanol-water (9:1, v/v) containing 0.1% formic acid. The column effluent was monitored by tandem mass spectrometry with electrospray ionization. The overall extraction efficiency was 50-60%, with values for precision and accuracy of < or =16% and <15% relative error, respectively. Our current method is approximately 60-100-fold more sensitive than previous assays, and will be used to define Tween 80 disposition in patients receiving Taxotere.
authors.affiliation: Laboratory of Experimental Chemotherapy and Pharmacology, Department of Medical Oncology, Rotterdam Cancer Institute (Daniel den Hoed Kliniek) and University Hospital Rotterdam, Groene Hilledijk 301, The Netherlands. sparreboom@onch.azr.nl
authors.forename: Alex
authors.initials: A
authors.lastname: Sparreboom
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Julie R
authors.initials: JR
authors.lastname: Brahmer
authors.forename: Jaap
authors.initials: J
authors.lastname: Verweij
authors.forename: Sharyn D
authors.initials: SD
authors.lastname: Baker
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000097:blood* / Q000627:therapeutic use; D002138:Calibration; D000077143:Docetaxel; D006801:Humans; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000097:blood* / Q000627:therapeutic use; D014677:Pharmaceutical Vehicles; D011136:Polysorbates / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D043823:Taxoids
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014677:Pharmaceutical Vehicles; D011136:Polysorbates; D043823:Taxoids; D000077143:Docetaxel; D017239:Paclitaxel