title: Phenotype markers and function of neutrophils in children with hemolytic uremic syndrome.
abstract: The hemolytic uremic syndrome (HUS) is the most-common cause of acute renal failure in children. Several researchers have reported the presence of neutrophil (PMN) activating cytokines, such as interleukin-8 and tumor necrosis factor-alpha, in the sera of HUS patients. Moreover, PMN-derived products, such as elastase, were increased. These observations have lead to the hypothesis that activated PMN could act as mediators of endothelial damage. The objective of this investigation was to directly evaluate the activation status of peripheral PMN from children with HUS. For this purpose, 12 children with typical HUS were bled during the acute period, before dialysis and/or transfusion, and 8 of them were also bled after 1 month follow-up. Additionally, blood samples from healthy control children admitted for routine surgical procedures, chronic uremic children, and neutrophilic children with acute infections not related to HUS were collected and processed in an identical manner. The function and membrane activation markers of PMN from these groups were evaluated. We found that during the acute period of HUS, PMN had reduced expression of FcgammaRIII (CD16) and CD11b, were degranulated, and exhibited an impaired antibody-dependent cellular cytotoxicity. These parameters returned to normal after clinical recuperation. We conclude that PMN activation in HUS patients is a very early and transient event, and upon hospitalization before dialysis PMN show a phenotype and functional pattern of partial deactivation.
authors.affiliation: División Inmunología, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Pacheco de Melo 3081, 1425, Buenos Aires, Argentina.
authors.forename: Gabriela C
authors.initials: GC
authors.lastname: Fernández
authors.forename: Carolina
authors.initials: C
authors.lastname: Rubel
authors.forename: Paula
authors.initials: P
authors.lastname: Barrionuevo
authors.forename: Laura
authors.initials: L
authors.lastname: López
authors.forename: Flavia
authors.initials: F
authors.lastname: Ramirez
authors.forename: Mario
authors.initials: M
authors.lastname: Díaz
authors.forename: Martín A
authors.initials: MA
authors.lastname: Isturiz
authors.forename: Marina S
authors.initials: MS
authors.lastname: Palermo
mesh_terms: D000293:Adolescent; D000328:Adult; D000920:Antibody-Dependent Cell Cytotoxicity; D015415:Biomarkers / Q000032:analysis; D015550:Cell Degranulation; D002462:Cell Membrane / Q000378:metabolism; D002648:Child; D002908:Chronic Disease; D005260:Female; D006463:Hemolytic-Uremic Syndrome / Q000097:blood*; D006801:Humans; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D010641:Phenotype; D017452:Receptors, IgG / Q000378:metabolism; D012016:Reference Values; D014511:Uremia / Q000097:blood
chemical_list: D015415:Biomarkers; D016177:Macrophage-1 Antigen; D017452:Receptors, IgG