[Purification and bioassay of macrophage-derived neurotrophic factor].
Macrophage-derived neurotrophic factor (M phi DNF) is purified from macrophage conditioned medium by a procedure consisting of column chromatography with Sephacryl S-100-HR, high-performance liquid chromatography (HPLC), and a final step using reverse-phase HPLC. The product shows a single protein band in sodium dodecyl sulfate-polyacrylamide gel. It has a molecular weight of 60.5 kD and an isoelectric point of pI 5.1 and contains more leucine, lysine, glutamine and aspartic acids in its amino acid composition. Purified M phi DNF can promote the survival, activity, and neurite outgrowth of cultured cerebellar cortical neurons and that this effect reaches maximal levels with concentrations of the M phi DNF ranging from 500-1000 ng/ml.
Li
S L
SL
Division of Neurosciences, Department of Histology and Embryology.
Guo
W H
WH
Gu
X F
XF
D000818:Animals; D002478:Cells, Cultured; D002525:Cerebellar Cortex / Q000166:cytology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017737:Macrophages, Peritoneal / Q000737:chemistry*; D051379:Mice; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000302:isolation & purification* / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects
D009414:Nerve Growth Factors