Screening of Arabidopsis thaliana stems for variation in cell wall polysaccharides.
A high-throughput method is described by which Arabidopsis thaliana stems can be screened for variation in cell wall composition after hydrolysis with Driselase or trifluoroacetic acid (TFA). Driselase, a mixture of fungal enzymes, hydrolyses cellulose (to glucose) and all the major matrix polysaccharides (to monosaccharides and/or characteristic disaccharides); TFA hydrolyses the matrix polysaccharides, but not cellulose, to monosaccharides. Two different wild-type ecotypes, Columbia and Wassilewskija, showed only minor differences in wall carbohydrate composition. A small number of T-DNA-tagged populations that were screened contained individuals in which the proportion of cellulose, xyloglucan or xylan differed quantitatively from the wild-type. Differences from the wild-type were also observed in the susceptibility of the hemicelluloses to hydrolysis by Driselase, probably reflecting differences in wall architecture.
Gardner
Sara L
SL
The Edinburgh Cell Wall Group, Institute of Cell and Molecular Biology, The University of Edinburgh, Daniel Rutherford Building, The King's Buildings, Mayfield Road, UK.
Burrell
M M
MM
Fry
Stephen C
SC
D000143:Acids / Q000737:chemistry; D017360:Arabidopsis / Q000737:chemistry*; D050260:Carbohydrate Metabolism; D002473:Cell Wall / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000032:analysis; D005656:Fungal Proteins; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D018547:Plant Stems / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis*; D014269:Trifluoroacetic Acid / Q000737:chemistry
D000143:Acids; D004269:DNA, Bacterial; D005656:Fungal Proteins; D011134:Polysaccharides; C036483:T-DNA; D014269:Trifluoroacetic Acid; D006026:Glycoside Hydrolases; C035212:driselase