Uterine, ovarian, and production responses of lactating dairy cows to increasing dietary concentrations of menhaden fish meal.
The primary objective was to determine whether the dietary polyunsaturated fatty acids, eicosapentaenoic (EPA, C20:5, n-3) and docosahexaenoic (DHA, C22:6, n-3), present in fish meal (FM) can attenuate uterine secretion of PGF2alpha in response to a challenge with estradiol and oxytocin in lactating dairy cows. Cycling multiparous cows (n = 32) were fed diets containing 0 (OFM), 2.6 (2.6FM), 5.2 (5.2FM), or 7.8% menhaden FM (7.8FM). The diet consisting of 7.8FM also contained fish oil (0.28% of dietary dry matter) to increase intake of EPA and DHA. Average dry matter intake was 24.9 kg/d and unaffected by diet. Combined intakes of EPA and DHA averaged 0, 12.8, 24.1, and 54.0 g/d from the OFM, 2.6FM, 5.2FM, and 7.8FM diets, respectively. At 30 to 34 d after initiation of dietary treatments, cows received an i.m. injection of 100 microg of GnRH followed by i.m. administration of 25 and 15 mg of PGF2alpha after 7 and 8 d, respectively. Synchronous ovulation was induced by an injection of 3000 IU of human chorionic gonadotropin (hCG) given 24 h later on d 9. Subsequent luteal phase increases in plasma progesterone concentrations did not differ (0.88 ng/ml per day). At 15 d after hCG injection, cows were injected with estradiol-17beta (3 mg, i.v.) at 0900 h and oxytocin (100 IU, i.v.) at 1300 h. Plasma PGF2alpha metabolite concentrations after oxytocin injection were reduced in cows fed diets containing FM compared with those fed OFM. Milk production (39.1 kg/d) and concentrations of fat, protein, or urea nitrogen in milk were not affected by diet. Feeding fish meal and fish oil containing eicosapentaenoic acid and docosahexaenoic acid reduced the proportion of n-6 fatty acids and increased that of n-3 fatty acids in milk in a dose-responsive manner.
Mattos
R
R
Department of Animal Sciences, University of Florida, Gainesville 32611-0920, USA.
Staples
C R
CR
Williams
J
J
Amorocho
A
A
McGuire
M A
MA
Thatcher
W W
WW
D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002417:Cattle / Q000502:physiology*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015118:Eicosapentaenoic Acid; D004958:Estradiol / Q000494:pharmacology; D004973:Estrus Synchronization; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage*; D005396:Fish Products; D007774:Lactation / Q000187:drug effects / Q000502:physiology; D008055:Lipids / Q000032:analysis; D008892:Milk / Q000737:chemistry / Q000378:metabolism; D008894:Milk Proteins / Q000187:drug effects / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D010121:Oxytocin / Q000494:pharmacology; D011374:Progesterone / Q000097:blood; D011897:Random Allocation; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D008055:Lipids; D008894:Milk Proteins; C033266:Menhaden oil; D004281:Docosahexaenoic Acids; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D011374:Progesterone; D004958:Estradiol; D010121:Oxytocin; D015118:Eicosapentaenoic Acid; D015237:Dinoprost