[Anti-inflammatory molecule, Clara cell 10 kilodalton protein and respiratory diseases].
Clara cell 10 kilodalton protein(CC10) is the major product of non-ciliated bronchiolar epithelial cells (Clara cells) and is identical with uteroglobin and urinary protein 1(P-1). CC10/uteroglobin is a homodimer consisting of 70 amino acid subunits arranged in an antiparallel fashion and connected by two disulfide bonds. CC10/uteroglobin shows an anti-inflammatory property including inhibition of phospholipase A2 and phospholipase C. We analyzed the epitope of monoclonal antibodies(mAbs) to human CC10/P-1. TY-5, TY-7 and TY-8 recognized alpha 1-, alpha 2- and alpha 3-helix of CC10, respectively. The combination of TY-1 and TY-2 are most suitable for ELISA, while TY-5 and TY-7 are very good for immunoblot and immunohistochemical analysis. Circulating CC10 levels are increased in sarcoidosis and decreased in asthma. Bronchoalveolar lavage CC10 levels are increased in sarcoidosis patients with a good outcome. The use of these mAbs is a powerful tool to investigate the clinical roles of CC10. CC10 is a regulator of the inflammatory process in respiratory diseases.
Shijubo
Noriharu
N
Third Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo 060-8543.
Itoh
Yoshihira
Y
Abe
Shosaku
S
D000911:Antibodies, Monoclonal / Q000276:immunology; D001249:Asthma / Q000175:diagnosis* / Q000503:physiopathology; D015415:Biomarkers / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000032:analysis* / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007118:Immunoassay; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D011379:Prognosis; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D017565:Sarcoidosis, Pulmonary / Q000175:diagnosis* / Q000503:physiopathology; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors; D014598:Uteroglobin
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D004791:Enzyme Inhibitors; D000939:Epitopes; D011506:Proteins; C556389:SCGB1A1 protein, human; D014598:Uteroglobin; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases