F2-isoprostanes biomarkers of lipid peroxidation: their utility in evaluation of oxidative stress induced by toxic agents.
Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of polyunsaturated fatty acids. There is a direct evidence showing that F2-isoprostanes can be utilized as a marker of lipid peroxidation due to the mechanism of their formation (nonenzymatic oxidation of arachidonic acid), chemical stability, sensitive and non-invasive methods of their estimation. An altered generation of F2-isoprostanes has been found in a variety of pathological syndromes associated with oxidative stress. Their quantification allows to elucidate the role of free radicals in oxidative injury. This paper reviews briefly the recent data on isoprostanes: biochemical mechanisms of their formation, methods of their measurement, and the possibilities of their utilization as a quantitative/qualitative marker of oxidative stress in vivo.
Piłacik
Bozena
B
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Lódź, Poland.
Nofer
Teresa Wrońska
TW
Wasowicz
Wojciech
W
D015415:Biomarkers / Q000032:analysis*; D006801:Humans; D028421:Isoprostanes / Q000032:analysis* / Q000096:biosynthesis; D015227:Lipid Peroxidation; D016273:Occupational Exposure; D018384:Oxidative Stress; D017382:Reactive Oxygen Species
D015415:Biomarkers; D028421:Isoprostanes; D017382:Reactive Oxygen Species