title: [Application of heteroduplex mobility assay in genetic subtyping on human immunodeficiency virus type 1].
abstract: OBJECTIVE
  Using heteroduplex mobility assay (HMA) to subtype human immunodeficiency virus type 1 (HIV-1) for the purpose of understanding HIV-1 subtype epidemic in Fujian province.
  
  
  METHODS
  DNA fragments of HIV-1 env gene were amplified from peripheral blood mononuclear cell (PBMC) cocultures of HIV-1 infected individuals by nested polymerase chain reaction (PCR). Heteroduplexs were formed through hybridizing PCR products from the samples and reference plasmid. According to the mobility of heteroduplexs in polyacrylamide gel electrophoresis, HIV-1 subtype from that sample was characterized and further confirmed by nucleotide sequencing analysis.
  
  
  RESULTS
  Thirteen of 15 (86.67%) samples were successfully subtyped by HMA, except 2 failures. Subtype E and B took up 80% (12/15) and 6.67% (1/15) respectively. Results indicated a high concordance between HMA and nucleotide sequencing analysis and concordance rate was 86.67% (13/15).
  
  
  CONCLUSIONS
  Subtype E appeared to be the major epidemic strain of HIV-1 in Fujian. HMA showed the characteristics of fastness, easiness, economic and with high specificity, and can be used in the surviellance for the epidemic strain of HIV-1.
authors.affiliation: Fujian Provincial Sanitary and Anti-Epidemic Station, Department of AIDS Prevention and Control, Fuzhou 350001, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Yan
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Weng
authors.forename: Huirong
authors.initials: H
authors.lastname: Wang
authors.forename: Shouli
authors.initials: S
authors.lastname: Wu
authors.forename: Ge
authors.initials: G
authors.lastname: Chen
authors.forename: Zhaoshuang
authors.initials: Z
authors.lastname: Zheng
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.forename: Pingping
authors.initials: P
authors.lastname: Yan
mesh_terms: D005838:Genotype; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D020180:Heteroduplex Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction