Biosensor for the enantioselective analysis of the thyroid hormones (+)-3,3',5-triiodo-L-thyronine (T3) and (+)-3,3',5,5'-tetraiodo-L-thyronine (T4).
An amperometric biosensor based on L-aminoacid oxidase is proposed for enantioselective assay of (+)-3,3',5-triiodo-L-thyronine (L-T3) and (+)-3,3',5,5'-tetraiodo-L-thyronine (L-T4), due to the fact that only the L enantiomer has the hormonal activity. The construction of the amperometric biosensor is simple and reproducible. The analytical information obtained from enantioselective analysis are reliable. The RSD <1% assured by using the amperometric biosensors for L enantiomers assay as raw materials, and from tablets, demonstrated their suitability for the analysis of T3 and T4 at ppb concentration levels.
Aboul-Enein
Hassan V
HV
Pharmaceutical Analysis Laboratory, Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. enein@kshrc.edu.sa
Stefan
Raluca-Ioana
RI
Litescu
Simona
S
Radu
Gabriel Lucian
GL
D000594:Amino Acid Oxidoreductases / Q000737:chemistry; D000818:Animals; D015374:Biosensing Techniques; D017839:Crotalus; D004563:Electrochemistry / Q000379:methods / Q000592:standards; D004800:Enzymes, Immobilized / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007457:Iodine Radioisotopes / Q000737:chemistry; D050830:L-Amino Acid Oxidase; D010592:Pharmaceutic Aids / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018030:Silver Compounds / Q000737:chemistry; D013237:Stereoisomerism; D013696:Temperature; D013974:Thyroxine / Q000032:analysis*; D013997:Time Factors; D014284:Triiodothyronine / Q000032:analysis*
D004800:Enzymes, Immobilized; D007457:Iodine Radioisotopes; D010592:Pharmaceutic Aids; D018030:Silver Compounds; D014284:Triiodothyronine; D006108:Graphite; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase; C037548:silver chloride; D013974:Thyroxine