title: Influence of anti-CD49f and anti-CD29 monoclonal antibodies on mitotic activity of epithelial cells (HaCaT) and gingival fibroblasts in vitro.
abstract: A major complication in the treatment of periodontitis marginalis is the reepithelization of periodontal defects inhibiting collagen fiber attachment and periodontal regeneration. In this study we investigated the possibility of a molecular blockade of epithelial mitosis in vitro. Monoclonal antibodies against the VLA-6 laminin receptor subunit alpha6 interrupted interactions between epithelial cells (HaCaT cells) and their extracellular matrix and thus resulted in reduction of proliferation rates by more than 50%. The same effect was observed with anti alpha1-antibodies. In contrast, collagen-producing and -secreting gingival fibroblasts, which play an important role in periodontal regeneration, remained unaffected by the applied anti alpha6 antibodies. Correspondingly, these cells were found to lack VLA-6 laminin receptors. Selective molecular inhibition of epithelial proliferation and apical migration by monoclonal anti alpha6 antibody application may provide an adjuvant periodontitis therapy resulting in an enhanced periodontal regeneration.
authors.affiliation: Department for Operative Dentistry, Periodontology and Preventive Dentistry, Medical Faculty, Rheinisch Westfälische Technische Hochschule (RWTH), Aachen, Germany. cbecker@ukaachen.de
authors.forename: Christoph
authors.initials: C
authors.lastname: Becker
authors.forename: Patricia
authors.initials: P
authors.lastname: Buttler
authors.forename: Hans Georg
authors.initials: HG
authors.lastname: Gräber
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015703:Antigens, CD / Q000502:physiology*; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects; D004846:Epithelial Attachment / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D039423:Integrin alpha1; D039121:Integrin alpha6beta1; D016023:Integrins / Q000037:antagonists & inhibitors / Q000502:physiology*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects*; D010519:Periodontium / Q000502:physiology; D017474:Receptors, Laminin / Q000096:biosynthesis / Q000502:physiology*; D012038:Regeneration
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039423:Integrin alpha1; D039121:Integrin alpha6beta1; D016023:Integrins; D017474:Receptors, Laminin