Induction of DNA damage by oxidised amino acids and proteins.
Exposure of amino acids, peptides and proteins to radicals in the presence of O2 generates hydroperoxides in a dose-dependent manner. These hydroperoxides are stable in the absence of exogenous catalysts (e.g. heat, light, redox-active transition metal ions), but decompose rapidly in the presence of these agents to give a variety of radicals including alkoxyl (RO*), peroxyl (ROO*) and carbon-centred (R) species. These radicals are shown to react with DNA to give DNA-protein cross-links and single strand breaks.
Luxford
Catherine
C
The Heart Research Institute, Sydney, NSW, Australia.
Dean
Roger T
RT
Davies
Michael J
MJ
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004249:DNA Damage; D004587:Electrophoresis, Agar Gel; D006861:Hydrogen Peroxide / Q000378:metabolism; D010084:Oxidation-Reduction; D011506:Proteins / Q000378:metabolism*
D000596:Amino Acids; D011506:Proteins; D006861:Hydrogen Peroxide