Immobilization of biocomponents for immune optical sensors.
Immobilisation of both human immunoglobulin(IgG) and antiimmunoglobulin (anti-IgG) was performed by means of polyelectrolyte self-assembly. This technique was compared with direct immobilisation of the immune components on bare gold and their covalent binding via glutaraldehyde as a bifunctional reagent. Additionally, the immune components were properly oriented during their immobilisation by using a predeposited layer of the protein A. Methods of the surface plasmon resonance (SPR) and planar interferometry were employed for monitoring the immobilisation as well as specific immune reaction. It was shown that in case of the use of polyelectrolyte self-assembly it is possible to achieve the sensitivity of the analysis up to 30 ng/ml for SPR and up to 1 ng/ml for planar interferometer based immune sensors.
Starodub
N F
NF
Palladin Institute of Biochemistry, NAS of Ukraine, Department of Sensory and Regulatory Systems. starodub@biochem.kiev.ua
Nabok
A V
AV
Starodub
V M
VM
Ray
A K
AK
Hassan
A K
AK
D001323:Autoantibodies / Q000276:immunology*; D015374:Biosensing Techniques; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance
D001323:Autoantibodies; D007074:Immunoglobulin G