Cyclin E and chromosome instability in colorectal cancer cell lines.
OBJECTIVE
The development of colorectal cancer depends on at least two distinct pathways involving genetic instability, namely: chromosome instability (CIN) and microsatellite instability. Cyclin E is involved in aneuploidy and several cancer types show an abnormal number of chromosomes.


METHODS
Cyclin E protein and mRNA values were analysed in human fetal skin fibroblasts and five colorectal cancer cell lines.


RESULTS
Cells with an aberrant number of chromosomes had higher cyclin E mRNA values and a significant increase in protein concentrations.


CONCLUSIONS
These data suggest that cyclin E regulation is altered in aneuploid cells and is an important factor in the CIN pathway.
Simone
C
C
Department of Internal and Public Medicine, Division of Medical Genetics, University of Bari, Bari 70124, Italy. cristianosimone@hotmail.com
Resta
N
N
Bagella
L
L
Giordano
A
A
Guanti
G
G
D002869:Chromosome Aberrations; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D019927:Cyclin E / Q000235:genetics* / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D014407:Tumor Cells, Cultured
D019927:Cyclin E; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm