title: Construction of heme enzymes: four approaches.
abstract: Construction of enzymes that catalyze either desired reactions or exhibit desired substrate specificity is one of the goals of enzymatic study. Rational design of enzymes is an important approach in this field. Another, extremely different, methodology from rational design, directed evolution, has been rapidly developed over the past two years.
authors.affiliation: Center for Integrative Bioscience and Institute for Molecular Science, Okazaki National Research Institutes, 444-8585, Okazaki, Japan. yoshi@ims.ac.jp
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D001665:Binding Sites; D002384:Catalysis; D003580:Cytochromes / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D019020:Directed Molecular Evolution / Q000379:methods; D006420:Hemeproteins / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics; D018716:Molecular Mimicry; D010544:Peroxidases / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D015202:Protein Engineering / Q000379:methods
chemical_list: D003580:Cytochromes; D006420:Hemeproteins; D010544:Peroxidases