title: In vivo imaging reveals different cellular functions for FGF and Dpp signaling in tracheal branching morphogenesis.
abstract: In the developing tracheal system of Drosophila melanogaster, six major branches arise by guided cell migration from a sac-like structure. The chemoattractant Branchless/FGF (Bnl) appears to guide cell migration and is essential for the formation of all tracheal branches, while Decapentaplegic (Dpp) signaling is strictly required for the formation of a subset of branches, the dorsal and ventral branches. Using in vivo confocal video microscopy, we find that the two signaling systems affect different cellular functions required for branching morphogenesis. Bnl/FGF signaling affects the formation of dynamic filopodia, possibly controlling cytoskeletal activity and motility as such, and Dpp controls cellular functions allowing branch morphogenesis and outgrowth.
authors.affiliation: Abteilung Zellbiologie, Biozentrum der Universität Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
authors.forename: Carlos
authors.initials: C
authors.lastname: Ribeiro
authors.forename: Andreas
authors.initials: A
authors.lastname: Ebner
authors.forename: Markus
authors.initials: M
authors.lastname: Affolter
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000502:physiology*; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics / Q000502:physiology; D009024:Morphogenesis; D011554:Pseudopodia / Q000502:physiology; D015398:Signal Transduction; D014132:Trachea / Q000254:growth & development*
chemical_list: D029721:Drosophila Proteins; D019476:Insect Proteins; C102644:bnl protein, Drosophila; C064196:dpp protein, Drosophila; D005346:Fibroblast Growth Factors