Rapid selection system of strains with higher avicel degrading ability in a cellulolytic fungus, trichoderma.
We have attempted to develop an active selection system for strains that have a higher potential for Avicel degradation using haploidized conidia from colchicine-treated Trichoderma reesei Rut C-30 as a model strain. Avicel, absorbent cotton, and wood powder were used as substrates for selection. It appeared that the strains that degrade Avicel actively could be effectively selected when the solid medium containing the selection substrate and the liquid medium containing Avicel were used.
Toyama
Hideo
H
Department of Food Science & Technology, Faculty of Horticulture, Minamikyushu University, Takanae, Miyazaki, Japan. gaf00771@nifty.com
Yamagishi
Naotsugu
N
Toyama
Nobuo
N
D001673:Biodegradation, Environmental; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D003368:Gossypium; D006238:Haploidy; D013379:Substrate Specificity; D014242:Trichoderma / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification; D014934:Wood
D002482:Cellulose; D002480:Cellulase