Influence of nutritional conditions on the mycelial growth and exopolysaccharide production in Paecilomyces sinclairii.
OBJECTIVE
The objective of the study was to optimize the submerged culture conditions for the production of exopolysaccharide from Paecilomyces sinclairii.


RESULTS
The optimal temperature and initial pH for exopolysaccharide production by Paecilomyces sinclairii in shake flask culture were found to be 30 degrees C and 6.0, respectively. Sucrose (60 g l(-1)) and corn steep powder (10 g l(-1)) were the most suitable carbon and nitrogen source for exopolysaccharide production.


CONCLUSIONS
Under optimal culture medium, the maximum exopolysaccharide concentration in a 5-l stirred-tank fermenter indicated 7.4 g l(-1), which was approximately three times higher than that in basal medium. The maximum specific growth rates (micro max) and yield coefficient (Y(P/S)) in the optimal culture medium was 0.16 h(-1) and 0.19, respectively.


CONCLUSIONS
The optimal culture conditions reported in this article can be widely applied to the processes for submerged cultures of other mushrooms.
Kim
S W
SW
Department of Biotechnology, Taegu University, Kyungsan, Kyungbuk, Korea.
Hwang
H J
HJ
Xu
C P
CP
Na
Y S
YS
Song
S K
SK
Yun
J W
JW
D002244:Carbon / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008903:Minerals / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010143:Paecilomyces / Q000254:growth & development / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D013696:Temperature
D003470:Culture Media; D008903:Minerals; D011134:Polysaccharides; D002244:Carbon; D009584:Nitrogen