Phorbol Ester Induces Formation of A New Phospholipase D Product.
It is demonstrated here that phorbol-12-myristate-13-acetate (PMA) induced the formation of a butanolated-sphingolipids in A-549 cells. In cells labeled with (3)H -serine, the distribution of the radioactivity was easily found inphosphatidylcholine (PC), phosphatidylserine (PS) and phosphatidylethanolamine (PE), but not in phosphatidic acid (PA) and its immediate metabolic derivatives, suggesting that the newly detected PLD products were derived from sphingolipids and were different from the conventional product of PLD. Protein kinase C (PKC)inhibitors or PKC down-regulation by prolonged PMA treatment inhibited the PMA-induced generation of this butanolated-sphingolipid, indicating that PKC activation was required in this process.
Chai
Min-Qiang
MQ
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China. songj@sunm.shcnc.ac.cn
Chen
Jun-Song
JS
Song
Jian-Guo
JG