Dynamics of reporter gene stimulation by HMG box proteins.
Overcoming local DNA rigidity is required to perform three-dimensional DNA-protein configuration at promoter regions. The abundant architectural nonhistone chromosomal HMG box proteins are nonsequence-specific; however, they have been established to specifically recognize distorted DNA. Using transient transfection to overexpress two different members of the HMGB-1/2 family of DNA architectural factors, we demonstrate that these proteins provide a general enhancement in reporter gene expression irrespective of the promoter being considered. Evidences are also provided indicating that stimulation may not be achieved by recruitment of the proteins by regulatory factors or as a consequence of major chromatin unfolding as previously suggested. Interestingly, the influence of the HMG box proteins under study was overridden when the promoters were either induced or stimulated by Trichostatin A (TSA) but recovered upon extended induction period. These results also support the concept that the architectural role of these proteins can contribute to the preinitiation complex assembly required for basal transcription, but to a much lesser extent to the poised promoter scaffolding characteristic of activated transcription.
Veilleux
Stéphane
S
Département de Biochimie, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada J1H 5N4.
Boissonneault
Guylain
G
D002460:Cell Line; D002875:Chromosomes / Q000737:chemistry; D017930:Genes, Reporter; D024002:HMGB Proteins / Q000037:antagonists & inhibitors / Q000502:physiology*; D024243:HMGB1 Protein / Q000502:physiology; D024261:HMGB2 Protein / Q000502:physiology; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D007700:Kinetics; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000502:physiology; D015533:Transcriptional Activation / Q000187:drug effects
D024002:HMGB Proteins; D024243:HMGB1 Protein; D024261:HMGB2 Protein; D006877:Hydroxamic Acids; D015534:Trans-Activators; C012589:trichostatin A