[Effect of cryoprotectors on binding of bromthymol blue by bovine methemoglobin].
Spectrophotometric method was used for study the binding of bromthymol blue dye (BTB) with bovine methemoglobin in 15% solutions of ethanol, glycerol and polyethylene glycol with molecular mass of 1.5 kDa (PEG-1500). It was shown, that adsorption of BTB by methemoglobin decreased in the sequence: glycerol > ethanol > PEG-1500. It is supposed that adsorption of the alcohols on the BTS sites of binding on methemolglobin led to the decrease of the amount of binding sites accessible for the dye.
Kucherenko
Iu V
IuV
Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkov.
Rozanova
E D
ED
D000818:Animals; D001979:Bromthymol Blue / Q000378:metabolism*; D002417:Cattle; D008706:Methemoglobin / Q000378:metabolism*; D008970:Molecular Weight; D011485:Protein Binding; D013057:Spectrum Analysis
D008706:Methemoglobin; D001979:Bromthymol Blue