Influence of anti-CD49f and anti-CD29 monoclonal antibodies on mitotic activity of epithelial cells (HaCaT) and gingival fibroblasts in vitro.
A major complication in the treatment of periodontitis marginalis is the reepithelization of periodontal defects inhibiting collagen fiber attachment and periodontal regeneration. In this study we investigated the possibility of a molecular blockade of epithelial mitosis in vitro. Monoclonal antibodies against the VLA-6 laminin receptor subunit alpha6 interrupted interactions between epithelial cells (HaCaT cells) and their extracellular matrix and thus resulted in reduction of proliferation rates by more than 50%. The same effect was observed with anti alpha1-antibodies. In contrast, collagen-producing and -secreting gingival fibroblasts, which play an important role in periodontal regeneration, remained unaffected by the applied anti alpha6 antibodies. Correspondingly, these cells were found to lack VLA-6 laminin receptors. Selective molecular inhibition of epithelial proliferation and apical migration by monoclonal anti alpha6 antibody application may provide an adjuvant periodontitis therapy resulting in an enhanced periodontal regeneration.
Becker
Christoph
C
Department for Operative Dentistry, Periodontology and Preventive Dentistry, Medical Faculty, Rheinisch Westfälische Technische Hochschule (RWTH), Aachen, Germany. cbecker@ukaachen.de
Buttler
Patricia
P
Gräber
Hans Georg
HG
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015703:Antigens, CD / Q000502:physiology*; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects; D004846:Epithelial Attachment / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D039423:Integrin alpha1; D039121:Integrin alpha6beta1; D016023:Integrins / Q000037:antagonists & inhibitors / Q000502:physiology*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects*; D010519:Periodontium / Q000502:physiology; D017474:Receptors, Laminin / Q000096:biosynthesis / Q000502:physiology*; D012038:Regeneration
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039423:Integrin alpha1; D039121:Integrin alpha6beta1; D016023:Integrins; D017474:Receptors, Laminin