beta-Adrenoceptor density and adenylyl cyclase activity in obese rabbit hearts.
OBJECTIVE
To determine whether decreased cardiac responsiveness to isoproterenol in obesity is associated with alterations in beta-receptors and/or adenylyl cyclase activity. ANIMALS AND DESIGN: After 12 weeks of control or ad libitum high-fat diets, left ventricular tissue from lean and obese female New Zealand white rabbits was assayed for beta-receptor binding density (11 lean, 11 obese) and isoproterenol-stimulated adenylyl cyclase activity (eight lean, 10 obese).


METHODS
Nonlinear least squares regression analysis was used to determine maximum density of beta-receptors and receptor affinity for (125)I-iodocyanopindolol. Four-parameter logistic regression was used to determine minimum, maximum, slope and EC(50) for isoproterenol-stimulated adenylyl cyclase activity.


RESULTS
Obese rabbits had elevated resting blood pressure and heart rate, and higher ventricular weights. However, beta-adrenoceptor density and affinity were not significantly different in lean and obese rabbits. Basal and maximum isoproterenol-stimulated adenylyl cyclase activity did not differ between lean and obese rabbits. In addition, maximal stimulation in response to sodium flouride did not differ between lean and obese. EC(50) for isoproterenol-stimulated adenylyl cyclase activity did not differ between lean and obese rabbits.


CONCLUSIONS
Obesity-related decreases in responsiveness of the isolated heart to isoproterenol are not associated with alterations in beta-receptor density and affinity. In addition, adenylyl cyclase activity appeared unchanged in ventricular preparations from obese rabbits. Decreased responsiveness to isoproterenol in obesity may be due to defects downstream of adenylyl cyclase activation of cyclic AMP.
Carroll
J F
JF
Department of Integrative Physiology, University of North Texas Health Science Center, Fort Worth, TX 76107, USA. jcarroll@hsc.unt.edu
Kyser
C K
CK
Martin
M M
MM
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure; D005260:Female; D006321:Heart / Q000187:drug effects; D006339:Heart Rate; D006352:Heart Ventricles / Q000473:pathology; D007457:Iodine Radioisotopes; D020120:Iodocyanopindolol / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D016015:Logistic Models; D009206:Myocardium / Q000737:chemistry* / Q000201:enzymology; D009765:Obesity / Q000201:enzymology* / Q000378:metabolism*; D009929:Organ Size; D011817:Rabbits; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000378:metabolism; D012044:Regression Analysis
D000318:Adrenergic beta-Agonists; D007457:Iodine Radioisotopes; D011943:Receptors, Adrenergic, beta; D020120:Iodocyanopindolol; D000262:Adenylyl Cyclases; D007545:Isoproterenol