title: High variability between results of different in-house tests for cytomegalovirus (CMV) monitoring and a standardized quantitative plasma CMV PCR assay.
abstract: A total of 2,718 blood samples were analyzed in five virological laboratories for the presence of cytomegalovirus (CMV) by in-house tests and one standardized plasma PCR assay. Results from in-house tests showed remarkable variability. Detection of CMV pp65 antigen or DNA from cells was more sensitive than that by plasma CMV PCR assay.
authors.affiliation: Abteilung Virologie, Universität Ulm, Ulm, Germany.
authors.forename: Lutz
authors.initials: L
authors.lastname: Von Müller
authors.forename: Walter
authors.initials: W
authors.lastname: Hampl
authors.forename: Joachim
authors.initials: J
authors.lastname: Hinz
authors.forename: Helga
authors.initials: H
authors.lastname: Meisel
authors.forename: Angela
authors.initials: A
authors.lastname: Reip
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Engelmann
authors.forename: Regine
authors.initials: R
authors.lastname: Heilbronn
authors.forename: Barbara
authors.initials: B
authors.lastname: Gärtner
authors.forename: Oliver
authors.initials: O
authors.lastname: Krämer
authors.forename: Hermann
authors.initials: H
authors.lastname: Einsele
authors.forename: Holger
authors.initials: H
authors.lastname: Hebart
authors.forename: Tatjana
authors.initials: T
authors.lastname: Ljubicic
authors.forename: Jürgen
authors.initials: J
authors.lastname: Löffler
authors.forename: Thomas
authors.initials: T
authors.lastname: Mertens
mesh_terms: D003470:Culture Media; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology; D004279:DNA, Viral / Q000097:blood*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006785:Hospitals, University; D006801:Humans; D007755:Laboratories, Hospital; D015588:Observer Variation; D010750:Phosphoproteins / Q000097:blood*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D015203:Reproducibility of Results; D014763:Viral Matrix Proteins / Q000097:blood*; D014773:Virology
chemical_list: D003470:Culture Media; D004279:DNA, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa