title: Deep lamellar keratoplasty with trypan blue intrastromal staining.
abstract: This technique was developed to facilitate removal of the deep stromal layers during deep lamellar keratoplasty. A trypan blue 0.02% solution is injected into the stromal fibers, enabling the surgeon to visualize and remove the posterior stromal layers. This decreases the risk of perforation of Descemet's membrane and the endothelium.
authors.affiliation: Department of Ophthalmology, University of L'Aquila, L'Aquila, Italy. anbalestrazzi@oculista.zzn.com
authors.forename: Emilio
authors.initials: E
authors.lastname: Balestrazzi
authors.forename: Angelo
authors.initials: A
authors.lastname: Balestrazzi
authors.forename: Luigi
authors.initials: L
authors.lastname: Mosca
authors.forename: Alessandra
authors.initials: A
authors.lastname: Balestrazzi
mesh_terms: D004396:Coloring Agents; D003316:Corneal Diseases / Q000175:diagnosis / Q000601:surgery; D003319:Corneal Stroma / Q000473:pathology* / Q000601:surgery*; D016039:Corneal Transplantation / Q000379:methods*; D006801:Humans; D007267:Injections; D013194:Staining and Labeling / Q000379:methods; D014343:Trypan Blue
chemical_list: D004396:Coloring Agents; D014343:Trypan Blue