D-amino acid oxidase: its potential in the production of 7-aminocephalosporanic acid.
D-Amino acid oxidase (DAAO) used in the preparation of alpha-keto acids, in the determination of D-amino acids and in the resolution of racemic mixture of amino acids is produced by a wide range of microorganisms. In the recent past this enzyme is being recognized for its potential in the commercial production of 7-aminocephalosporanic acid (7-ACA), a starting material for various semisynthetic cephalosporins. Though this enzyme is widespread among microorganisms, very few microbial species have been explored for the production of 7-ACA; this is because cephalosporin C is quantitatively deaminated by limited microbial DAAOs. Comparison of physico-chemical properties of enzyme preparations indicate wide variations, however in general DAAOs are specific for D-configuration of amino acids. Both immobilized enzyme and cell preparations are developed for its various applications. The advantages of DAAO in the production of 7-ACA are discussed.
Mujawar
S K
SK
Biochemistry Division, Research and Development, Hindustan Antibiotics Ltd., Pimpri, Pune 411 018, India.
D000818:Animals; D001419:Bacteria / Q000201:enzymology / Q000378:metabolism; D002511:Cephalosporins / Q000096:biosynthesis* / Q000737:chemistry; D003605:D-Amino-Acid Oxidase / Q000096:biosynthesis / Q000737:chemistry*; D006801:Humans
D002511:Cephalosporins; C030735:7-aminocephalosporanic acid; D003605:D-Amino-Acid Oxidase