Effects of arginine, L-alanyl-L-glutamine or taurine on neutrophil (PMN) free amino acid profiles and immune functions in vitro.
The objective of this study was to determine the effects of arginine, L-alanyl-L-glutamine (Ala-Gln) or taurine on polymorphonuclear leucocyte (PMN) free amino acid profiles, superoxide anion (O2-) generation, hydrogen peroxide (H2O2) formation and released myeloperoxidase activity (MPO). Arginine led to significant increases in PMN arginine, ornithine, citrulline, aspartate, glutamate and alanine concentrations as well as increased H2O2-generation and MPO activity while O(2-)-formation was decreased. Ala-Gln caused significant increases in PMN free glutamine, alanine, asparagine, aspartate, glutamate, ornithine, arginine, serine and glycine concentrations and increased PMN immune functions. Taurine significantly increased PMN free taurine profiles, reduced PMN neutral amino acid content and decreased H2O2- and O(2-)-formation while MPO was increased. Altogether, the pharmacological regimens which enhance the supply of arginine, Ala-Gln or taurine in whole blood significantly affect PMN "susceptible free amino acid pool". This may be one of the determinants in PMN nutrition considerably influencing PMN immune functions. Introduction Polymorphonuclear leucocytes (PMN) ensure an important part of non-specific cell-mediated immunity and play a crucial role in the host defense
Mühling
J
J
Department of Anaesthesiology and Intensive Care Medicine, Justus Liebig University, Giessen, Federal Republic of Germany. joerg.muehling@chiru.med.uni-giessen.de
Fuchs
M
M
Fleck
C
C
Sablotzki
A
A
Krüll
M
M
Dehne
M G
MG
Gonter
J
J
Weiss
S
S
Engel
J
J
Hempelmann
G
G
D000328:Adult; D000596:Amino Acids / Q000097:blood; D001120:Arginine / Q000494:pharmacology*; D002853:Chromatography, Liquid; D004151:Dipeptides / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology*
D000596:Amino Acids; D004151:Dipeptides; D013654:Taurine; D001120:Arginine; D009195:Peroxidase; C054122:alanylglutamine