Detection of a variant metallo-beta-lactamase, IMP-10, from two unrelated strains of Pseudomonas aeruginosa and an alcaligenes xylosoxidans strain.
The gene bla(IMP-10) of a variant metallo-beta-lactamase, IMP-10, had a single base replacement of G by T at nucleotide 145, which led to an amino acid alteration of Val49 to Phe compared to the IMP-1 enzyme, indicating that IMP-10 was a point mutation derivative of IMP-1. Highly purified enzymes revealed that IMP-10 was different from IMP-1 in its extremely low hydrolyzing activities for penicillins, such as benzylpenicillin, ampicillin, and piperacillin.
Iyobe
Shizuko
S
Laboratory of Drug Resistance in Bacteria, Gunma University School of Medicine, Maebashi, Japan. siyobe@med.gunma-u.ac.jp
Kusadokoro
Haruko
H
Takahashi
Ayako
A
Yomoda
Sachie
S
Okubo
Toyoji
T
Nakamura
Akio
A
O'Hara
Koji
K
D000421:Alcaligenes / Q000201:enzymology*; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D015378:Imipenem / Q000494:pharmacology; D007700:Kinetics; D010957:Plasmids / Q000235:genetics; D017354:Point Mutation / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet; D013845:Thienamycins / Q000494:pharmacology; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
D004269:DNA, Bacterial; D013845:Thienamycins; D015378:Imipenem; C466680:metallo-beta-lactamase, IMP-10; D001618:beta-Lactamases