Expression of intestinal ornithine decarboxylase during postnatal development in neonatal rats.
Ornithine decarboxylase (ODC) has been shown to play an essential role in intestinal growth and maturation in rats. However, the regulatory mechanisms have not been fully elucidated. We studied the mechanisms of expression of intestinal ODC during postnatal development. Rat small intestinal mucosa was obtained from postnatal days 10, 15, 17, 19, 21, 24 and 30. Intestinal mucosa was assayed for ODC and sucrase activities. In addition, intestinal ODC mRNA, and ODC protein levels were also measured. The results showed that the intestinal sucrase activity was low before postnatal day 19. The sucrase activity then increased steadily from day 19 up to day 30. Intestinal ODC activities remained low from postnatal day 10 to day 17. A sharp increase in ODC activity was noted on day 19, which peaked on day 24 (a 20-fold increase from its low basal level) and declined on day 30. Intestinal ODC proteins followed the same pattern of postnatal expression as that of ODC activity. In contrast, ODC mRNA did not show significant change throughout the study period. The possible mechanisms by which intestinal ODC mRNA levels remain practically unchanged during postnatal development are discussed. We conclude that the ontogenic increase in sucrase activity, a marker for intestinal maturation, occurs at the same time to that of the induction of ODC activity. We also suggest that the induction of intestinal ODC activity during postnatal development is the result of post-transcriptional events or other cellular mechanisms. A better understanding of the regulation of polyamine biosynthesis during postnatal development of the small intestine will provide insights contributing to the maturation of the small intestine.
Lin
Chuan-Hao
CH
Department of Pediatrics, Division of Gastroenterology and Nutrition, Wayne State University and Children's Hospital of Michigan, 3901 Beaubien Blvd., Detroit 48201, USA. lchuan@med.wayne.edu
Vijesurier
Roy
R
Ho
Ye-Shih
YS
Schipper
Raymond G
RG
Tolia
Vasundhara
V
Moshier
Jeffrey A
JA
Majumdar
Adhip P N
AP
D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D007413:Intestinal Mucosa / Q000201:enzymology / Q000254:growth & development; D007421:Intestine, Small / Q000201:enzymology / Q000254:growth & development*; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013393:Sucrase / Q000096:biosynthesis
D012333:RNA, Messenger; D013393:Sucrase; D009955:Ornithine Decarboxylase