title: Development of a genetic selection for catalytic antibodies.
abstract: The design and evaluation of a new genetic selection system for evolving catalytic antibodies with aldolase activity are described. Through a series of model selections, we have identified selection conditions where expression of a catalytically active antibody confers a growth advantage to Escherichia coli. In addition, we provide evidence that the growth advantage is a direct result of catalytic activity.
authors.affiliation: Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Jeff
authors.initials: J
authors.lastname: Gildersleeve
authors.forename: Jeff
authors.initials: J
authors.lastname: Janes
authors.forename: Helle
authors.initials: H
authors.lastname: Ulrich
authors.forename: Priscilla
authors.initials: P
authors.lastname: Yang
authors.forename: Carlos
authors.initials: C
authors.lastname: Barbas
authors.forename: Peter G
authors.initials: PG
authors.lastname: Schultz
mesh_terms: D017151:Antibodies, Catalytic / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000254:growth & development; D005634:Fructose-Bisphosphate Aldolase / Q000276:immunology*; D012641:Selection, Genetic
chemical_list: D017151:Antibodies, Catalytic; D017931:DNA Primers; D005634:Fructose-Bisphosphate Aldolase