title: The sequential model of Barrett's esophagus and adenocarcinoma induced by duodeno-esophageal reflux without exogenous carcinogens.
abstract: BACKGROUND
  The experiment was designed to sequentially examine the histogenesis of Barrett's esophagus and esophageal adenocarcinoma induced by duodenal reflux without exogenous carcinogens.
  
  
  METHODS
  Wistar male rats, 200 in all, each weighing approximately 250 g were used. The totally gastrectomized animals were reconstructed by Schlatter's method to produce duodeno-esophageal reflux (n = 100), for comparison with no reflux, Roux-en-Y reconstruction (n = 100). The excised esophagus was histopathologically examined every 10 weeks after surgery until 50 weeks.
  
  
  RESULTS
  Among the animals with reflux, Barrett's epithelium developed near esophago-jejunostoma 10 weeks after surgery and subsequently spread upward. Columnar dysplasia was first identified in the zone of Barrett's epithelium at 20 weeks, simultaneously with adenocarcinoma. As the incidence of Barrett's esophagus increased over time, the incidence of both dysplasia and adenocarcinoma also increased. Adenocarcinoma developed in the area of columnar dysplasia.
  
  
  CONCLUSIONS
  The temporal progression from Barrett's esophagus to columnar dysplasia and adenocarcinoma is induced by duodeno-esophageal reflux. Columnar dysplasia is a morphological marker for adenocarcinogenesis.
authors.affiliation: Department of Second Surgery, School of Medicine Kanazawa University, Ishikawa, Japan. YIB03163@niftyserve.or.jp
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sato
authors.forename: Koichi
authors.initials: K
authors.lastname: Miwa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sahara
authors.forename: Masataka
authors.initials: M
authors.lastname: Segawa
authors.forename: Takanori
authors.initials: T
authors.lastname: Hattori
mesh_terms: D000230:Adenocarcinoma / Q000209:etiology* / Q000473:pathology; D000818:Animals; D001471:Barrett Esophagus / Q000209:etiology* / Q000473:pathology; D004195:Disease Models, Animal; D004383:Duodenogastric Reflux / Q000150:complications* / Q000473:pathology; D004386:Duodenum / Q000601:surgery; D004938:Esophageal Neoplasms / Q000209:etiology* / Q000473:pathology; D004947:Esophagus / Q000601:surgery; D005743:Gastrectomy; D008297:Male; D051381:Rats