A radiation hybrid map of bovine X chromosome (BTAX).
We present a comprehensive radiation hybrid map of the bovine X chromosome (Chr) containing 20 new markers, including both microsatellites and expressed genes. This study was conducted with a 5000-rad whole genome RH cell panel consisting of 90 hybrid cell lines. Retention frequencies of individual markers range from 7.8% for XIST to 31.1% for TGLA325. Statistical analysis with RHMAPPER placed all the loci into five linkage groups under a LOD score criterion of 6.0. These groups could be oriented relative to each other because they included multiple microsatellite loci from the consensus linkage map of the X Chr. Markers included in both this RH map and the bovine cytogenetic map were in a consistent order. The comparative bovine-human map thus generated consists of five blocks of genes, the order of which is conserved, although in the opposite direction when presented as ideograms with p and q arms. Inversions of three blocks account for the difference in gene order across the entirety of the two X Chrs.
Amaral
M Elisabete J
ME
Departamento de Biologia, IBILCE - UNESP, São José Rio Preto, SP, 15054-000, Brazil.
Kata
Srinivas R
SR
Womack
James E
JE
D000818:Animals; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D016133:Polymerase Chain Reaction / Q000662:veterinary; D021101:Radiation Hybrid Mapping / Q000662:veterinary*; D014960:X Chromosome / Q000235:genetics*
D005819:Genetic Markers; D004247:DNA