Immunogenicity of a recombinant lumpy skin disease virus (neethling vaccine strain) expressing the rabies virus glycoprotein in cattle.
Rabies virus (RV) readily infects cattle and causes a fatal neurological disease. A stable vaccine, which does not require the maintenance of a cold chain and that is administered once to elicit lifelong immunity to rabies would be advantageous. The present study describes the construction of a live recombinant lumpy skin disease virus (LSDV) vaccine, expressing the glycoprotein of rabies virus (RG) and assessment of its ability to generate a humoral and cellular immune response against rabies virus in cattle. Cattle inoculated with the recombinant virus (rLSDV-RG) developed humoral immunity that was demonstrated in ELISA and neutralisation assays to RV. High titres of up to 1513IU/ml of RV neutralising antibodies were induced. In addition, peripheral blood mononuclear cells from rLSDV-RG-immunised animals demonstrated the ability to proliferate in response to stimulation with inactivated RV, whereas the animal vaccinated with wild type LSDV did not. This recombinant vaccine candidate thus has the potential to be used in ruminants as a cost-effective vaccine against both lumpy skin disease (LSD) and rabies.
Aspden
Kate
K
Division of Medical Virology, Department Clinical Laboratory Sciences, Faculty of Health Sciences, University of Cape Town, Observatory, Cape Town 7925, South Africa.
van Dijk
Alberdina A
AA
Bingham
John
J
Cox
Dermot
D
Passmore
Jo-Ann
JA
Williamson
Anna-Lise
AL
D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral; D002417:Cattle; D018929:Cell Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D017930:Genes, Reporter; D005822:Genetic Vectors; D006023:Glycoproteins / Q000276:immunology*; D007111:Immunity, Cellular; D008167:Lumpy skin disease virus / Q000235:genetics / Q000276:immunology*; D011819:Rabies Vaccines / Q000276:immunology*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*; D001616:beta-Galactosidase / Q000378:metabolism
D000956:Antigens, Viral; D006023:Glycoproteins; D011819:Rabies Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus; D001616:beta-Galactosidase