[Simultaneous determination of the enantiomers of verapamil and norverapamil in plasma by high performance capillary electrophoresis].
An analytical method has been developed to determine simultaneously plasma concentration of the enantiomers of verapamil and its major metabolite, norverapamil, using capillary zone electrophoresis. Trimethyl-beta-cyclodextrin was selected as chiral selector. Good separation was achieved under the conditions as below: Running electrolyte was pH 2.5, 60 mmol.L-1 phosphate buffer containing 60 mmol.L-1 trimethyl-beta-cyclodextrin; capillary: 30 cm x 75 microns (ID), coated; setpoint of capillary temperature: 20 degrees C; detector: UV 200 nm; injection: electromigration at 12 kV for 7 s; running voltage: 14 kV. Solvent of sample was doubly deionized water. The migration times of enantiomers were less than 12 min. The good linear range was from 300.0 ng.ml-1 to 2.50 ng.ml-1. Relative standard deviations of assay accuracy and precision were less than 12%. It fits for routine therapeutic monitoring as it is highly efficient, rapid, sensitive, with less solvent and sample consumption and automation.
Rui
J
J
Department of Clinical Pharmacology, Jinling Hospital, Nanjing 210002.
Zhou
X
X
Lin
S
S
Yuan
Y
Y
Xiang
B
B
An
D
D
D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D008297:Male; D013237:Stereoisomerism; D014700:Verapamil / Q000031:analogs & derivatives* / Q000097:blood*
C016904:norverapamil; D014700:Verapamil