Expression of tumor necrosis factor ligand superfamily costimulatory molecules CD27L, CD30L, OX40L and 4-1BBL in the heart of patients with acute myocarditis and dilated cardiomyopathy.
BACKGROUND
T-cell-mediated myocardial damage is known to be involved in acute myocarditis and dilated cardiomyopathy. Recently, we found that tumor necrosis factor (TNF) ligand superfamily costimulatory molecules, especially 4-1BBL, played an important role in the myocardial damage of murine acute viral myocarditis.


RESULTS
To investigate the roles for CD27L, CD30L, OX40L and 4-1BBL, which belong to TNF ligand superfamily, in the development of acute myocarditis and dilated cardiomyopathy, we analyzed the expression of these antigens in the myocardial tissues of patients with acute myocarditis and dilated cardiomyopathy. We also examined expression of the receptors for these molecules, CD27, CD30, OX40 and 4-1BB, which belong to TNF receptor superfamily, on the infiltrating cells. Strong expression of CD27L, CD30L and 4-1BBL and weak to moderate expression of OX40L was found in the cardiac myocytes of patients with acute myocarditis. Moderate expression of CD27L, CD30L and 4-1BBL and weak expression of OX40L was found on the cardiac myocytes of patients with dilated cardiomyopathy. Most of the infiltrating cells expressed CD27, CD30 and 4-1BB and a part of the infiltrating cells expressed OX40.


CONCLUSIONS
Our findings suggest that expression of TNF ligand superfamily costimulatory molecules on cardiac myocytes may play a role in the cell-mediated myocardial damage in patients with acute myocarditis and dilated cardiomyopathy as in murine viral myocarditis.
Seko
Yoshinori
Y
Department of Cardiovascular Medicine, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Yokyo 113-8655, Japan. sekoyosh-tky@umin.ac.jp
Ishiyama
Shigeru
S
Nishikawa
Toshiro
T
Kasajima
Takeshi
T
Hiroe
Michiaki
M
Suzuki
Shin
S
Ishiwata
Sugao
S
Kawai
Sachio
S
Tanaka
Yuetsu
Y
Azuma
Miyuki
M
Kobata
Tetsuji
T
Yagita
Hideo
H
Okumura
Ko
K
Nagai
Ryozo
R
D053330:4-1BB Ligand; D000208:Acute Disease; D015703:Antigens, CD; D053282:CD27 Ligand; D053285:CD30 Ligand; D002311:Cardiomyopathy, Dilated / Q000378:metabolism* / Q000473:pathology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008875:Middle Aged; D009205:Myocarditis / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D053278:OX40 Ligand; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D053330:4-1BB Ligand; D015703:Antigens, CD; D053282:CD27 Ligand; D053285:CD30 Ligand; C505771:CD70 protein, human; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D053278:OX40 Ligand; C089251:TNFSF4 protein, human; C505774:TNFSF8 protein, human; C505974:TNFSF9 protein, human; D014409:Tumor Necrosis Factor-alpha