Studies on the interaction between Cd(2+) ions and nucleobases and nucleotides.
Cadmium is a potent carcinogen in rodents and has recently been accepted by the International Agency for Research on Cancer as a category 1 (human) carcinogen, but the molecular mechanism of its action remains largely unclear. It has however been suggested that cadmium-induced carcinogenesis may involve either direct or indirect interaction of Cd(2+) with DNA. In this study it is found that when Cd(2+) is allowed to interact with adenine and guanine, there is a marked change in the high performance liquid chromatography (HPLC) retention time for adenine but not for guanine. Since Cd(2+) is believed to bind covalently to adenine and guanine, the changes in retention time but absence of any cadmium in the peak fraction point to the following: (i) lability of cadmium-nucleobase adducts, and (ii) introduction of some kind of chemical modification in adenine but not in guanine as a result of covalent binding. This result is different from that for Ni(2+) in which case a change in retention time was observed for guanine but not for adenine.
Hossain
Zahed
Z
School of Biomedical Sciences, Faculty of Health Sciences C42, PO Box 170, The University of Sydney, East Street, NSW 1825, Lidcombe, Australia
Huq
Fazlul
F
D000225:Adenine / Q000737:chemistry / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002104:Cadmium / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D004247:DNA / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D003848:Deoxyguanine Nucleotides / Q000737:chemistry / Q000378:metabolism; D006147:Guanine / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D015394:Molecular Structure; D017382:Reactive Oxygen Species / Q000378:metabolism; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared
D003848:Deoxyguanine Nucleotides; D017382:Reactive Oxygen Species; D002104:Cadmium; D000249:Adenosine Monophosphate; D006147:Guanine; C007257:2'-deoxyguanosine 5'-phosphate; D004247:DNA; D000225:Adenine