Preferential transduction of human hepatocytes with lentiviral vectors pseudotyped by Sendai virus F protein.
One of the major challenges facing gene therapy is the development of vectors targeting specific cell types. Restricting gene delivery to the relevant cell type leads to reduced T-cell responses to transgene products and prolonged gene expression. In this study, we demonstrate that vectors derived from human immunodeficiency virus (HIV) can be pseudotyped with Sendai virus fusion protein F. Such vectors transduced human hepatoma cells and primary human hepatocytes efficiently, but not non-liver cells. Several different approaches were also taken to significantly increase the titer of the pseudotyped vector. These studies may facilitate HIV vector-mediated gene delivery into liver in vivo.
Kowolik
Claudia M
CM
Department of Virology, Beckman Research Institute, City of Hope, 1500 E. Duarte Road, Duarte, California 91010, USA.
Yee
Jiing-Kuan
JK
D037263:Asialoglycoprotein Receptor / Q000378:metabolism; D005822:Genetic Vectors; D006678:HIV / Q000235:genetics*; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D029082:Sendai virus; D014161:Transduction, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
D037263:Asialoglycoprotein Receptor; C099057:F0 protein, Sendai virus; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins