Genes induced by growth hormone in a model of adipogenic differentiation.
A substantial number of GH regulated genes have been reported in mature hepatocytes, but genes involved in GH-initiated cell differentiation have not yet been identified. Here we have studied a well-characterised model of GH-dependent differentiation, adipogenesis of 3T3-F442A preadipocytes, to identify genes rapidly induced by GH. Using the suppression subtractive hybridisation technique, we have identified eight genes induced within 60 min of GH treatment, and verified these by northern analysis. Six were identifiable as Stat 2, Stat 3, thrombospondin-1, oncostatin M receptor beta chain, a DEAD box RNA helicase, and muscleblind, a developmental transcription factor. Bioinformatic approaches assigned one of the two remaining unknown genes as a novel 436 residue serine/threonine kinase. As each of the identified genes have important developmental roles, they may be important in initiating GH-induced adipogenesis.
Shang
Catherine A
CA
Department of Physiology and Pharmacology, Institute for Molecular Biology, University of Queensland, Qld 4072, Brisbane, Australia.
Thompson
Barry J L
BJ
Teasdale
Rohan
R
Brown
Richard J
RJ
Waters
Michael J
MJ
D017667:Adipocytes / Q000502:physiology*; D000595:Amino Acid Sequence; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D015723:Gene Library; D013006:Growth Hormone / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism; D053677:Receptors, Oncostatin M; D050795:STAT2 Transcription Factor; D050796:STAT3 Transcription Factor; D019700:Thrombospondin 1 / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D018121:Receptors, Cytokine; D053677:Receptors, Oncostatin M; D050795:STAT2 Transcription Factor; C494084:STAT2 protein, human; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D019700:Thrombospondin 1; D015534:Trans-Activators; C109271:mbl protein, Drosophila; D013006:Growth Hormone; D017346:Protein Serine-Threonine Kinases; D020365:RNA Helicases