Measurement of alpha-tocopherol turnover in plasma and in lipoproteins using stable isotopes and gas chromatography/mass spectrometry.
Burton and Daroszewska (16) have presented an excellent method for quantifying alpha-tocopherol in human and animal tissues. The present paper expands that method by including the theory and calculations for alpha-tocopherol turnover in human plasma and lipoprotein fractions. Recent advances in mathematical modeling in experimental nutrition (22) have been aided by the increased availability of labeled isotopes and sensitive analytical methods. Applied to the study of alpha-tocopherol, these techniques will allow the characterization of the kinetic behavior of this micronutrient in vivo and expand the understanding of this key nutrient's role in preventing disease.
Parks
Elizabeth J
EJ
Department of Food Science and Nutrition, University of Minnesota, Twin Cities, St. Paul, MN, USA.
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007554:Isotopes / Q000378:metabolism; D008074:Lipoproteins / Q000378:metabolism*; D024502:alpha-Tocopherol / Q000032:analysis* / Q000097:blood
D007554:Isotopes; D008074:Lipoproteins; D024502:alpha-Tocopherol