Immunohistochemical evaluation of the effects of sodium hypochlorite on dentin collagen and glycosaminoglycans.
To test the hypothesis that a commercial solution of 5% NaOCl produces structural and molecular alterations in the collagen and glycosaminoglycans of mineralized and demineralized dentin, radicular segments from human teeth were treated with 5% NaOCl or 2 min at room temperature. As a control, distilled water replaced NaOCl. The experimental and control specimens were processed for indirect immunofluorescence by using antitype I collagen and antichondroitin sulfate antibodies. Tissue sections were morphometrically analyzed. A single exposure for 2 min to a 5% NaOCl solution produced a drastic loss of immunoreactivity in the dentin surface for both antibodies that were used in demineralized specimens. A narrow and irregular band of fluorescence loss was detected in mineralized-dentin segments when anticollagen antibodies were used. The results of this study suggest that 5% NaOCl induces alterations in dentin collagen and glycosaminoglycans and show the protective role of hydroxyapatite on organic matrix stability.
Oyarzún
Alejandro
A
Unit of BioStructure, Faculty of Odontology, University of Chile, Santiago.
Cordero
Ana M
AM
Whittle
Marta
M
D000328:Adult; D024042:Collagen Type I / Q000737:chemistry / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects*; D003806:Dentin Permeability / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry; D005109:Extracellular Matrix / Q000187:drug effects; D019084:Fluorescent Antibody Technique, Indirect; D006025:Glycosaminoglycans / Q000737:chemistry; D006801:Humans; D016877:Oxidants / Q000633:toxicity*; D012388:Root Canal Irrigants / Q000633:toxicity*; D012973:Sodium Hypochlorite / Q000633:toxicity*
D024042:Collagen Type I; D006025:Glycosaminoglycans; D016877:Oxidants; D012388:Root Canal Irrigants; D017886:Durapatite; D012973:Sodium Hypochlorite