Comparison of different DNA preparation protocols for PCR diagnosis of Human African Trypanosomosis in Côte d'Ivoire.
During a medical survey the sleeping sickness focus in Bonon, Ivory Coast, PCR with Trypanosoma brucei specific primers (TBR 1-2 from Parasitology 99 (1989) 57) was tested on DNA derived from blood samples. DNA purification using a chelating resin was performed either on whole blood or on the buffy coat prepared in two different ways. The preparation based on whole blood performed better than those using the buffy-coat. Using this first method, the sensitivity was 100% on parasitologically confirmed patients, and the specificity was 92%. However, problems of reproducibility of the technique were pointed out, particularly on samples from serologically positive but apparently aparasitemic individuals. It is suggested that the PCR could help in the diagnosis of Human African Trypanosomosis, but the use of other primers should be investigated.
Solano
P
P
Institut Pierre Richet, IRD UR 035, BP 1500, Bouake, Ivory Coast. solano@ird.ci
Jamonneau
V
V
N'Guessan
P
P
N'Dri
L
L
Dje
N N
NN
Miezan
T W
TW
Lejon
V
V
Büscher
P
P
Garcia
A
A
D000818:Animals; D007560:Cote d'Ivoire; D016054:DNA, Protozoan / Q000302:isolation & purification*; D004492:Edetic Acid; D006493:Heparin; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014347:Trypanosoma brucei gambiense; D014353:Trypanosomiasis, African / Q000175:diagnosis*
D016054:DNA, Protozoan; D006493:Heparin; D004492:Edetic Acid