title: Connexin 43 suppresses human glioblastoma cell growth by down-regulation of monocyte chemotactic protein 1, as discovered using protein array technology.
abstract: Previously, we demonstrated that connexin 43 (cx43) suppressed the growth of human glioblastoma cells. To investigate the molecular mechanisms involved in tumor suppression by cx43, we developed a human cytokine array system, which simultaneously detects the expression of 43 cytokines. By using this new technology, we analyzed the cx43-regulated genes in cx43-transfected cells. The cytokine arrays showed that expression of monocyte chemotactic protein-1 (MCP-1) was profoundly reduced in cx43-transfected cells. RT-PCR, immuno-Western blot, and cDNA microarrays further confirmed this observation. Addition of conditioned medium from control-transfected cells and recombinant MCP-1 to cx43-transfected cells significantly enhanced cx43-transfected cell proliferation and colony formation in soft agar. In contrast, addition of neutralization antibody against MCP-1 significantly inhibited cell proliferation in control-transfected cells. Our results suggested that MCP-1 is involved in the suppression of human glioblastoma cell growth by cx43.
authors.affiliation: Department of Gynecology and Obstetrics, Emory University School of Medicine, 1639 Pierce Drive, Atlanta, GA 30322, USA.
authors.forename: Ruochun
authors.initials: R
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
authors.forename: Cheng C
authors.initials: CC
authors.lastname: Wang
authors.forename: Jacob
authors.initials: J
authors.lastname: Gano
authors.forename: Biaoyang
authors.initials: B
authors.lastname: Lin
authors.forename: Qian
authors.initials: Q
authors.lastname: Shi
authors.forename: Alton
authors.initials: A
authors.lastname: Boynton
authors.forename: Jocelyn
authors.initials: J
authors.lastname: Burke
authors.forename: Ruo-Pan
authors.initials: RP
authors.lastname: Huang
mesh_terms: D002455:Cell Division / Q000502:physiology; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D018031:Connexin 43 / Q000235:genetics / Q000502:physiology*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D015536:Down-Regulation; D020869:Gene Expression Profiling; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D014162:Transfection
chemical_list: D018932:Chemokine CCL2; D018031:Connexin 43; D016207:Cytokines