FA composition of heart and skeletal muscle during embryonic development of the king penguin.
Since the yolk lipids of the king penguin (Aptenodytes patagonicus) naturally contain the highest concentrations of DHA and EPA yet reported for the eggs of any avian species, the effects of this (n-3)-rich yolk on the FA profiles of the embryonic heart and skeletal muscle were investigated. The concentrations (mg/g wet tissue) of phospholipid (PL) in the developing heart and leg muscle of the penguin doubled between days 27 and 55 from the beginning of egg incubation (i.e., from the halfway stage of embryonic development to 2 d posthatch), whereas no net increase occurred in pectoral muscle. During this period, the concentration of TAG in heart decreased by half but increased two- and sixfold in leg and pectoral muscle, respectively. The most notable change in cholesteryl ester concentration occurred in pectoral muscle, increasing ninefold between days 27 and 55. Arachidonic acid (ARA) was the major polyunsaturate in PL of the penguin's heart, where it formed about 20% (w/w) of FA at day 55. At the equivalent developmental stage, the heart PL of the chicken contained a 1.3-fold greater proportion of ARA, contained a fifth less DHA, and was almost devoid of EPA, whereas the latter FA was a significant component (7% of FA) of penguin heart PL. Similarly, in PL of leg and pectoral muscle, the chicken displayed about 1.4-fold more ARA, up to 50% less DHA, and far less EPA in comparison with the penguin. Thus, although ARA-rich PL profiles are achieved in the heart and muscle of the penguin embryo, these profiles are significantly affected by the high n-3 content of the yolk.
Decrock
Frederic
F
Centre d'Ecologie et Physiologie Energétiques, Centre National de la Recherche Scientifique, Associé à l'Université Louis Pasteur, Strasbourg, France.
Groscolas
Rene
R
Speake
Brian K
BK
D000818:Animals; D001717:Birds; D002642:Chick Embryo; D005314:Embryonic and Fetal Development; D006321:Heart / Q000196:embryology*; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*