[Determination of oligopeptide by IR matrix-assisted laser desorption ionization mass spectrometry].
Luteinizing releasing hormone analog (LRH-A), renin substrate tetradecapeptide and angiotensin I were analyzed with infrared-matrix assisted laser desorption ionization/Fourier transform ion cyclotron resonance mass spectrometry (IR-MALDI/FTICR MS). 2,5-Dihydroxybenzoic (DHB) and succinic acid (SA) were used as matrices. The m/z of oligopeptides were determined with an error less than 4 x 10(-6). A series of characteristic fragment ions of peptides were obtained by increasing the laser power density appropriately. Their elemental compositions and the first order structures were confirmed. A sensitive, rapid and accurate method was established for the determination of oligopeptide.
Zhou
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H
Analytical and Computer Center, China Pharmaceutical University, Nanjing 210009.
Sheng
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D000595:Amino Acid Sequence; D000803:Angiotensin I / Q000032:analysis; D000808:Angiotensinogen / Q000032:analysis; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis; D009842:Oligopeptides / Q000032:analysis*; D011487:Protein Conformation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D009842:Oligopeptides; D000808:Angiotensinogen; D007987:Gonadotropin-Releasing Hormone; D000803:Angiotensin I