A single amino acid substitution in 126-kDa protein of Pepper mild mottle virus associates with symptom attenuation in pepper; the complete nucleotide sequence of an attenuated strain, C-1421.
The complete nucleotide sequence of an attenuated Pepper mild mottle virus (PMMoV C-1421) RNA genome has been determined. There were two differences from the type isolate in Japan (PMMoV-J). The mutations were located in the middle of the 126-kDa protein (126 K) gene; one mutation influenced amino acid substitution at 649th Val to Ala (V649A), and the other was silent. The analyses using the reverse genetic system of PMMoV-J revealed that symptom attenuation on pepper related to V649A. Accumulations of 126 K and coat protein (CP) in V649A mutant-infected pepper were lower than those of PMMoV-J in immunoblotting. These results suggest that V649A substitution in 126 K affects the accumulation of 126 K leading to a limitation of CP accumulation.
Hagiwara
K
K
National Agricultural Research Center, Tsukuba, Ibaraki, Japan.
Ichiki
T U
TU
Ogawa
Y
Y
Omura
T
T
Tsuda
S
S
D019943:Amino Acid Substitution; D002212:Capsicum / Q000191:economics / Q000821:virology*; D002213:Capsid / Q000032:analysis / Q000096:biosynthesis; D016679:Genome, Viral; D015151:Immunoblotting; D007564:Japan; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000191:economics / Q000821:virology*; D012324:RNA-Dependent RNA Polymerase / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D017898:Tobamovirus / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D014633:Valine / Q000235:genetics
D012324:RNA-Dependent RNA Polymerase; D014633:Valine