A multiplicity of erythrocyte glycolipids of the neolacto series revealed by immuno-thin-layer chromatography with monoclonal anti-I and anti-i antibodies.
The thin-layer-chromatography immunostaining procedure was applied to human erythrocyte glycolipids using monoclonal anti-i and anti-I antibodies which are directed against epitopes on linear and branched carbohydrate chains of the neolacto (poly-N-acetyllactosamine) series. An examination of native and mild-acid-treated glycolipids from normal adult (I(adult) antigen type), neonatal (i(cord)), and I-antigen-deficient adult (i(adult)) erythrocytes enabled certain structural inferences to be made as follows: (a) cells of both I and i phenotypes contain a multiplicity of glycolipids of the neolacto series whose backbones consist of 8 or more sugar residues; (b) the octasaccharide backbones are predominantly linear in cells of i phenotype and branched in those of I type; and (c) more complex glycolipids having decasaccharide and larger backbones with both linear and branched sequences occur in erythrocytes of both phenotypes.
Uemura
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Applied Immunochemistry Research Group, Clinical Research Centre, Harrow, Middlesex, UK.
Childs
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RA
Hanfland
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P
Feizi
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T
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002855:Chromatography, Thin Layer / Q000379:methods*; D000939:Epitopes; D004912:Erythrocytes / Q000737:chemistry* / Q000276:immunology; D004913:Erythrocytes, Abnormal / Q000737:chemistry / Q000276:immunology; D006017:Glycolipids / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007048:I Blood-Group System / Q000276:immunology*; D007231:Infant, Newborn; D051379:Mice; D009844:Oligosaccharides / Q000737:chemistry; D011134:Polysaccharides / Q000276:immunology*; D012015:Reference Standards; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006017:Glycolipids; D007048:I Blood-Group System; D009844:Oligosaccharides; D011134:Polysaccharides; C037199:poly-N-acetyllactosamine