Determination of matrine and oxymatrine in Sophora subprostata by CE.
Shan-dou-gen is the dried roots of Sophora subprostata (Leguminosae) and a commonly used Chinese herbal drug in Taiwan. It possesses antipyretic, anti-inflammatory, analgesic effects and is used to treat sore throat and acute pharyngolaryngeal infections. To evaluate the quality of S. subprostata, a simple, rapid and accurate high-performance capillary electrophoresis (HPCE) method was developed for the assay of two alkaloids: matrine and oxymatrine. The electrolyte was a buffer solution containing 75% 130 mM phosphate buffer (NaH2PO4/H3PO4, pH 3.5) and 25% acetonitrile. Applied voltage was 10 kV and temperature was 30 degrees C. 2-(4-Hydroxyphenyl)ethylammonium chloride was used as an internal standard and detector set at 200 nm. The contents of matrine and oxymatrine of S. subprostata in several different samples of crude drugs and commercial concentrated preparation have also been determined.
Ku
Yoe-Ray
YR
Department of Health, National Laboratories of Foods and Drugs, Executive Yuan 161-2, Kuen-Yang Street, Nankang, Taipei, Taiwan, ROC.
Chang
Li-Yun
LY
Lin
Jer-Huei
JH
Ho
Li-Kang
LK
D000470:Alkaloids / Q000032:analysis*; D002138:Calibration; D019075:Electrophoresis, Capillary; D007887:Fabaceae / Q000737:chemistry*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D018517:Plant Roots / Q000737:chemistry; D011807:Quinolizines; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013624:Taiwan; D000093842:Matrines
D000470:Alkaloids; D007202:Indicators and Reagents; D011807:Quinolizines; D012996:Solutions; C037573:oxymatrine; D000093842:Matrines