[Detection of inv (16) in acute myelomonocytic leukemia by interphase fluorescence in situ hybridization].
OBJECTIVE
To evaluate fluorescence in situ hybridization (FISH) in the detection of inv (16) (p13; q22).


METHODS
Spectrum red labeled yeast artificial chromosome (YAC) clone 854E2 which spans the breakpoint cluster region in MYH11 in band 16p13 and single color interphase FISH were used to detect inv (16) in 26 cases of acute myelomonocytic leukemias (AML-M(4)), and the results were compared with that of conventional cytogenetic analysis.


RESULTS
R banding karyotyping test revealed no inv (16) in 25 cases, one AML M(4Eo) case showed inv (16) by G banding. Nine cases including all three M(4Eo) had inv (16) by FISH analysis, among whom the characteristic fluorescence signal pattern of the inv (16) was seen in 13.3% to 32.1% (median, 21.3%) of the tested cells.


CONCLUSIONS
YAC 854E2 and interphase FISH provide a powerful technique in the detection of inv (16) (p13q22).
Li
Jianyong
J
First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou 215006, China.
Pan
Jinlan
J
Wu
Yafang
Y
Guo
Yu
Y
Xue
Yongquan
Y
D000328:Adult; D000368:Aged; D007446:Chromosome Inversion; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase / Q000235:genetics; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged