Heat stress protection against mesenteric I/R-induced alterations in intestinal mucosa in rats.
Prior induction of heat shock protein 70 (HSP70) protects against ischemia-reperfusion (I/R) mucosal injury, but the ability of HSP70 to affect I/R-induced alterations in epithelial cell function is unknown. Rats subjected to whole body hyperthermia (41.5-42 degrees C for 6 min) increased HSP70 and heat shock factor 1 mRNA expression, reaching a maximum 2 h after heat stress and declining thereafter. HSP70 production was maximally elevated at 4 h after heat stress and remained elevated until after 12 h. Heat stress alone had no effect on mucosal function except to enhance secretion in response to ACh. Heat stress provided complete morphological protection against I/R-induced mucosal injury but did not confer a similar protection against I/R-induced decreases in mucosal resistance, sodium-linked glucose absorption, or tachykinin-mediated chloride secretion. Heat stress, however, attenuated the I/R-induced suppression of ACh response, and this effect was dependent on enteric nerves. Thus induction of heat shock protein 70 is associated with the preservation of mucosal architecture and attenuation of some specific functional alterations induced by I/R.
Fleming
Sherry D
SD
Department of Cellular Injury, Walter Reed Army Medical Institute of Research, Silver Spring, Maryland, USA.
Starnes
Benjamin W
BW
Kiang
Juliann G
JG
Stojadinovic
Alexander
A
Tsokos
George C
GC
Shea-Donohue
Terez
T
D000042:Absorption; D000109:Acetylcholine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D002712:Chlorides / Q000378:metabolism; D017615:Enteric Nervous System / Q000502:physiology; D005947:Glucose / Q000493:pharmacokinetics; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature; D006979:Hyperthermia, Induced; D007413:Intestinal Mucosa / Q000473:pathology* / Q000503:physiopathology; D007511:Ischemia / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology* / Q000503:physiopathology; D013152:Splanchnic Circulation; D013312:Stress, Physiological / Q000503:physiopathology*; D013779:Tetrodotoxin / Q000494:pharmacology
D002712:Chlorides; D018840:HSP70 Heat-Shock Proteins; D013779:Tetrodotoxin; D005947:Glucose; D000109:Acetylcholine