title: Impaired noradrenaline-induced lipolysis in white fat of aP2-Ucp1 transgenic mice is associated with changes in G-protein levels.
abstract: In vitro experiments suggest that stimulation of lipolysis by catecholamines in adipocytes depends on the energy status of these cells. We tested whether mitochondrial uncoupling proteins (UCPs) that control the efficiency of ATP production could affect lipolysis and noradrenaline signalling in white fat in vivo. The lipolytic effect of noradrenaline was lowered by ectopic UCP1 in white adipocytes of aP2-Ucp1 transgenic mice, overexpressing the UCP1 gene from the aP2 gene promoter, reflecting the magnitude of UCP1 expression, the impaired stimulation of cAMP levels by noradrenaline and the reduction of the ATP/ADP ratio in different fat depots. Thus only subcutaneous but not epididymal fat was affected. UCP1 also down-regulated the expression of hormone-sensitive lipase and lowered its activity, and altered the expression of trimeric G-proteins in adipocytes. The adipose tissue content of the stimulatory G-protein alpha subunit was increased while that of the inhibitory G-protein alpha subunits decreased in response to UCP1 expression. Our results support the idea that the energy status of cells, and the ATP/ADP ratio in particular, modulates the lipolytic effects of noradrenaline in adipose tissue in vivo. They also demonstrate changes at the G-protein level that tend to overcome the reduction of lipolysis when ATP level in adipocytes is low. Therefore, respiratory uncoupling may exert a broad effect on hormonal signalling in adipocytes.
authors.affiliation: Department of Adipose Tissue Biology and Center for Integrated Genomics, Institute of Physiology, Academy of Sciences of the Czech Republic, Vídenská 1803, 142 20 Prague, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Flachs
authors.forename: Jirí
authors.initials: J
authors.lastname: Novotný
authors.forename: Filip
authors.initials: F
authors.lastname: Baumruk
authors.forename: Kristina
authors.initials: K
authors.lastname: Bardová
authors.forename: Lenka
authors.initials: L
authors.lastname: Bourová
authors.forename: Ivan
authors.initials: I
authors.lastname: Miksík
authors.forename: Jana
authors.initials: J
authors.lastname: Sponarová
authors.forename: Petr
authors.initials: P
authors.lastname: Svoboda
authors.forename: Jan
authors.initials: J
authors.lastname: Kopecký
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000273:Adipose Tissue / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism; D017931:DNA Primers; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D005838:Genotype; D022781:Hepatocytes / Q000201:enzymology; D007473:Ion Channels; D008049:Lipase / Q000235:genetics; D008066:Lipolysis; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D024101:Mitochondrial Proteins; D009638:Norepinephrine / Q000378:metabolism*; D000071256:Uncoupling Protein 1
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D008049:Lipase; D019204:GTP-Binding Proteins; D009638:Norepinephrine