Lead induced spermatoxicity in mouse and MPG treatment.
Protective efficacy of MPG (2-mercaptopropionyl glycine) was studied against the toxic effects of lead acetate in Swiss albino mice. The animals were treated with single dose of lead acetate @ 180, 200 and 250 mg/kg b.wt. in presence and absence of MPG. The results indicated that the body weight was slightly higher in MPG treated groups on day 10 as compared to only respective lead treated groups in all the three dose level. However, significantly lower body weight was observed in both lead treated and lead along with MPG treated groups as compared to control. Patten of mortality is similar in both lead treated and lead plus MPG treated groups. Conspicuous degenerative changes in testicular tissues and elevation in sperm head shape abnormality were observed in both lead treated and lead along with MPG treated groups but the sperm head shape abnormality and damage were more in lead treated groups as compared to lead plus MPG treated groups. But this difference was non-significant between the two groups. These observations suggest that MPG may not be significantly effective against lead induced damage in testicular tissues at cellular level. However, MPG is able to maintain slightly lower level of sperm abnormality in all the three dose level as compared to their respective lead treated groups. Further, studies are needed to find out the optimum dose of MPG for protection against the lower doses of lead induced lethality as MPG is not significantly effective against the higher doses of lead.
Gautam
A K
AK
Division of Reproductive Toxicology, National Institute of Occupationa Health, Meghani Nagar, Ahmedabad, India.
Agarwal
K
K
Shah
B A
BA
Kumar
S
S
Saiyed
H N
HN
D000818:Animals; D001835:Body Weight; D004305:Dose-Response Relationship, Drug; D007263:Infusions, Parenteral; D008297:Male; D051379:Mice; D009942:Organometallic Compounds / Q000633:toxicity*; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000002:abnormalities; D013737:Testis / Q000187:drug effects / Q000473:pathology*; D008625:Tiopronin / Q000494:pharmacology*
D009942:Organometallic Compounds; D008625:Tiopronin; C008261:lead acetate