[Effect of N-palmitoylethanolamine on phospholipid and fatty acid level in ischemic rat liver under reperfusion].
The effect of 10(-5) M N-palmitoylethanolamine (NPE) on lipid peroxidation processes, phospholipid and fatty acid content in the ischemic rat liver under reperfusion was estimated. It was shown the significant accumulation of thiobarbituric acid reactive substances (TBARS), the increase of lysophosphatidylethanolamine (LPC), phosphatidylserine, phosphatidylethanolamine, sphingomyelin, general cholesterol content and also the decrease of phosphatidylcholine amount in the ischemic rat liver under reperfusion. Simultaneously the redistribution of saturated and unsaturated fatty acids quantity was detected, that indicates the increasing of membrane rigidity. The addition of NPE into the Eurocollins and Bretshneider's solutions under perfusion and preservation of a donor organ reduced the accumulation of TBARS, LPC, total cholesterol, prevented the changes of cholesterol/phospholipids and saturated/unsaturated fatty acids ratio value in the same experimental conditions. These effects, evidently, formed the basis of the protective action of NPE on the ischemic liver tissues under reperfusion.
Hula
N M
NM
Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv. ngula@biochem.kiev.ua
Hordid'ko
T M
TM
Marhitych
V M
VM
Hovseieva
N M
NM
Klimashevs'kyĭ
V M
VM
Shahidulin
M Iu
MIu
D000577:Amides; D000818:Animals; D063388:Endocannabinoids; D004983:Ethanolamines; D005227:Fatty Acids / Q000378:metabolism*; D015227:Lipid Peroxidation; D008099:Liver / Q000098:blood supply*; D008297:Male; D010169:Palmitic Acids / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000577:Amides; D063388:Endocannabinoids; D004983:Ethanolamines; D005227:Fatty Acids; D010169:Palmitic Acids; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances; C005958:palmidrol