Dendritic cell-based proliferative assays of peripheral T cell responses to tetanus toxoid.
Antigen-specific proliferative responses of peripheral blood T cells are widely used in humans to study the T cell compartment. However, responses to autoantigens are often weak and poorly reproducible. Here we show, using a test recall antigen (tetanus toxoid), that absolute levels of proliferation, minimally detectable antigen doses, and/or ability to detect statistically significant responses can be enhanced using in vitro-generated autologous dendritic cells as antigen presenting cells.
Narendran
Parth
P
University Department of Medicine, Bristol Royal Infirmary, Bristol BS2 8HW, UK. parth@narendran.co.uk
Elsegood
Kathryn
K
Leech
Nicola J
NJ
Dayan
Colin M
CM
D017951:Antigen Presentation; D001324:Autoantigens / Q000032:analysis* / Q000276:immunology*; D002455:Cell Division; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*; D014611:Vaccination
D001324:Autoantigens; D013745:Tetanus Toxoid