Identification of the lymphocytic choriomeningitis virus (LCMV) proteins required to rescue LCMV RNA analogs into LCMV-like particles.
We have used a reverse genetic approach to identify the viral proteins required for packaging and assembly of the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV). Plasmids encoding individual LCMV proteins under the control of an RNA polymerase II promoter were cotransfected with a plasmid containing an LCMV minigenome (MG). Intracellular synthesis of the LCMV MG was driven by T7 RNA polymerase whose expression was also mediated by a Pol II promoter. The supernatant from transfected cells was passaged onto fresh cells that were subsequently infected with LCMV to provide the minimal viral trans-acting factors, NP and L, that are required for LCMV MG RNA replication and expression. Reconstitution of LCMV-specific packaging and passage was detected by expression of the chloramphenicol acetyl transferase (CAT) reporter gene present in the MG. NP and L did not direct detectable levels of MG passage. Addition of Z and GP resulted in high levels of passage of CAT activity, which could be prevented by LCMV neutralizing antibodies. Passage of LCMV MG was inhibited by omission of either GP or Z.
Lee
Ki Jeong
KJ
Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 92037, USA.
Perez
Mar
M
Pinschewer
Daniel D
DD
de la Torre
Juan Carlos
JC
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism*; D016679:Genome, Viral; D006023:Glycoproteins / Q000378:metabolism*; D008217:Lymphocytic choriomeningitis virus / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D012319:RNA Polymerase II / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014764:Viral Proteins / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000378:metabolism*; D014771:Virion / Q000235:genetics / Q000378:metabolism*
D000956:Antigens, Viral; D006023:Glycoproteins; D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins; C115679:glycoprotein peptide, Lymphocytic choriomeningitis virus; D012319:RNA Polymerase II