Cell enlargement of plant tissue explants oscillates with a temperature-compensated period of ca. 24 min.
Rate of plant cell enlargement, measured at intervals of 3 min using a sensitive linear transducer, oscillates with a minimum period of about 24 min that parallels the 24-min periodicity observed with the oxidation of NADH by the external plasma membrane NADH oxidase and of single cells measured previously by video-enhanced light microscopy. Also exhibiting 24-min oscillations is the steady-state rate of cell enlargement induced by the addition of the auxin herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) or the natural auxin indole-3-acetic acid (IAA). Immediately following 2,4-D addition, a very complex pattern of oscillations is frequently observed. However, after several hours a dominant 24-min period emerges. The length of the 24-min period is temperature compensated and remains constant at 24 min when measured at 15, 25 or 35 degrees C, despite the fact that the rate of cell enlargement approximately doubles for each 10 degree C rise over this same range of temperatures.
Morre
D James
DJ
Department of Medicinal Chemistry, Purdue University, West Lafayette, IN 47907-1333, USA. Morre@pharmacy.purdue.edu
Ternes
Philipp
P
Morre
Dorothy M
DM
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D048429:Cell Size / Q000187:drug effects; D018548:Cotyledon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005583:Fourier Analysis; D006540:Herbicides / Q000494:pharmacology; D018546:Hypocotyl / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007210:Indoleacetic Acids / Q000494:pharmacology; D009097:Multienzyme Complexes / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010507:Periodicity; D019441:Plant Epidermis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D013025:Glycine max / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D003313:Zea mays / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D006540:Herbicides; D007210:Indoleacetic Acids; D009097:Multienzyme Complexes; D010937:Plant Growth Regulators; D009243:NAD; D015084:2,4-Dichlorophenoxyacetic Acid; C030737:indoleacetic acid; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases