title: Quantification of sensitivity of endothelial cell markers for the astrocytoma and oligodendroglioma tumours.
abstract: Angiogenesis, the formation of new blood vessels, is necessary for tumour growth. The aim of this study was to quantify the sensitivity of five endothelial cell markers CD31/CD34-mix, CD31, CD34, lectin UEA-1 and FVIII-rag. Paraffin-embedded tumour tissue from 15 patients with astrocytomas grades II-IV and oligodendrogliomas grades II-III was used Sections of 2 microm were stained. Seven serial sections from each of the 15 tumours enabled consecutive examination of the vessels with all five markers and with two interposed hematoxylin-eosin (H&E) sections as controls. Among the endothelial cell markers, the mix CD31/CD34 showed the highest sensitivity with 79% and 99%, respectively, when applied in astrocytomas grade II and grades III-IV and 86% when applied in oligodendrogliomas. The study has shown that the sensitivity of the five endothelial cell markers varied. The mix CD31/CD34 was superior and seems to be a useful marker for quantification of angiogenesis.
authors.affiliation: Department of Neuroscience, Neurology, Uppsala University, Sweden. dhana.aronsson@medcellbiol.uu.se
authors.forename: Dhana E
authors.initials: DE
authors.lastname: Aronsson
authors.forename: Carin
authors.initials: C
authors.lastname: Muhr
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D001254:Astrocytoma / Q000098:blood supply* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001932:Brain Neoplasms / Q000098:blood supply* / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism; D009837:Oligodendroglioma / Q000098:blood supply* / Q000473:pathology; D016612:Paraffin Embedding; D037121:Plant Lectins; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D012680:Sensitivity and Specificity; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D037102:Lectins; D037121:Plant Lectins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C036850:Ulex europaeus lectins; D014841:von Willebrand Factor