Effect of kLa on the production of glucose 6-phosphate dehydrogenase from Saccharomyces cerevisiae grown by fermentation process.
In a 5-L fermentor (NBS-MF 105), Saccharomyces cerevisiae (0.7 g/L) was inoculated into a liquid medium (pH 4.0) containing 17 g/L of glucose, 2.55 g/L of yeast extract, 4.25 g/L of peptone, 2.04 g/L of Na2HPO4 x 12H2O, 4.34 g/L of (NH4)2SO4 and 0.064 g/L of MgSO4 x 7H2O and aerobically cultivated at 35 degrees C for 22 h. Agitation and aeration were adjusted to attain initial kLa values of 15, 60, 135, and 230 h(-1). The glucose 6-phosphate dehydrogenase (G6PDH) productivity (PrG6PDH) obtained for kLa values of 15, 60, 135, and 230 h(-1) was 10.6, 31.8, 30.3, and 23.3 U/([Lx h]), respectively, whereas the cell productivity (Pr(x)) for the same kLa values were 0.24, 0.69, 0.69, and 0.49 g/[L x h], respectively. Thus, both events are coupled and depend on the dissolved oxygen in the medium.
Silva
Daniel Pereira
DP
Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmacy, Univeristy of São Paulo, SP, Brazil.
Pessoa
Adalberto
A
Roberto
Inês C
IC
Vitolo
Michele
M
D005285:Fermentation; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D008962:Models, Theoretical; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development*
D005954:Glucosephosphate Dehydrogenase