Human recombinant EGF protein delivered by a biodegradable cell transplantation system.
We have previously shown a new approach to expand cultured human keratinocytes and reconstitute the epidermis in full-thickness wounds using a new microsperical transport system. This was a new approach to increase the cell yield for seeding without altering the anchoring proteins by enzymatic steps. That time we used Cytodex 3 which failed to be degraded and induced an inflammatory reaction in a t-cell-deficient organism. Therefore, we have investigated another microcarrier consisting of PLGA, which is a well-known carrier material for cell culture and transplantation. After coating the PLGA carrier with gelatine the seeding time of viable cells reached 4 h and the cell gain after 7 days of spinner culture was 16-fold. At 14 days after transplantation, we could detect a new stratified epithelium in our full-thickness wound healing model. Because cytokines play a major role in wound healing, we loaded this carrier material with different concentrations of rhEGF, showing a dose dependent release of the protein in vitro and in vivo. This result might lead to a different approach in the treatment of wounds.
Voigt
M
M
Department of Plastic and Hand Surgery, University of Freiburg Medical Center, Germany. voigt@ch11.ukl.uni-freiburg.de
Andree
C
C
Kalt
T
T
Dörmann
S
S
Schaefer
D J
DJ
Walgenbach
K-J
KJ
Stark
G B
GB
D020341:Absorbable Implants; D000328:Adult; D000818:Animals; D001672:Biocompatible Materials; D017690:Cell Transplantation / Q000379:methods*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004815:Epidermal Growth Factor / Q000008:administration & dosage*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects*; D019344:Lactic Acid; D051379:Mice; D008819:Mice, Nude; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D011994:Recombinant Proteins / Q000008:administration & dosage; D014945:Wound Healing / Q000187:drug effects
D001672:Biocompatible Materials; D004337:Drug Carriers; D011108:Polymers; D011994:Recombinant Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004815:Epidermal Growth Factor