Formation of dTDP-rhamnose is essential for growth of mycobacteria.
It was determined that the dTDP-rhamnose synthesis gene, rmlD, could be inactivated in Mycobacterium smegmatis only in the presence of a rescue plasmid carrying functional rmlD. Hence, dTDP-rhamnose biosynthesis is essential for the growth of mycobacteria and the targeting of dTDP-rhamnose synthesis for new tuberculosis drugs is supported.
Ma
Yufang
Y
Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA.
Pan
Fei
F
McNeil
Michael
M
D002237:Carbohydrate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D020102:Mycobacterium smegmatis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D010957:Plasmids; D011995:Recombination, Genetic; D013942:Thymine Nucleotides / Q000096:biosynthesis*
D003470:Culture Media; D009702:Nucleoside Diphosphate Sugars; D013942:Thymine Nucleotides; C073293:thymidine diphosphate rhamnose; D002237:Carbohydrate Dehydrogenases; C107144:dTDP-4-dehydrorhamnose reductase