Lymphatic absorption of docosahexaenoic acid given as monoglyceride, diglyceride, triglyceride, and ethyl ester in rats.
Lymphatic absorption of docosahexaenoic acids (DHA) given as monoglyceride (MG), consisting of 1(or 3)-species (9 1.4%), 2-species (4.2%) and diglyceride (DG) consisting of 1,3-species (70.8%), 1(or 3),2-species (28.6%), were investigated in comparison with that of triglyceride (TG) and ethyl ester (EE). Rats were infused with a lipid emulsion containing 200 mg of DHA-MG, DG, TG, or EE via a gastric cannula. Lymph was collected through the thoracic lymph duct at 2 h intervals for 10 h and at a single collection from 10 to 30 h. Physiological saline containing glucose was infused (2 mL/h) throughout the lymph collection. The overall recovery of DHA at 30 h after its infusion was significantly higher in the rank order DHA-MG>DG>TG=EE. Moreover, time-dependent changes in recovery rates from 2 to 10 h of DHA given as MG were significantly higher than those of the corresponding DG, TG, and EE. These results indicate that DHA-MG and DG are absorbed and transported more effectively than TG and EE forms under restricted water supply, even if they mainly consist of 1(or 3)-species. Lymph lipids were mainly transported as TG, and a large amount of DHA was incorporated into a TG fraction in all fat types examined. Furthermore, the intramolecular distribution of DHA in lymph TG was similar in all groups. docosahexaenoic acid, monoglyceride, diglyceride triglyceride, ethyl ester
Banno
Fumiaki
F
Graduate School of Agricultural Science, Tohoku University, Aoba, Sendai, Japan.
Doisaki
Shinji
S
Shimizu
Nobutoshi
N
Fujimoto
Kenshiro
K
D000704:Analysis of Variance; D000818:Animals; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D004952:Esters / Q000008:administration & dosage / Q000378:metabolism; D005227:Fatty Acids / Q000008:administration & dosage / Q000378:metabolism; D005989:Glycerides / Q000378:metabolism*; D008196:Lymph / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013897:Thoracic Duct / Q000378:metabolism
D004952:Esters; D005227:Fatty Acids; D005989:Glycerides; D004281:Docosahexaenoic Acids