title: Characterization of gibberellin producing strains of Fusarium moniliforme based on DNA polymorphism.
abstract: The gibberellins are one of the major groups of growth promoting hormones and are secondary metabolites of the fungus Fusarium moniliforme (Perfect stage: Gibberella fujikuroi). Sixteen strains of Fusarium from different geographical regions and different hosts were analysed for their ability to produce gibberellins (GA) and for genetic relatedness by random amplified polymorphic DNA (RAPD). Range of gibberellin production varied between 28.9 to 600.0 mg g(-1) dry weight of mycelium in different strains of Fusarium. RAPD analysis showed completely different pattern between high, moderate and low producing strains. High producers formed nearly identical RAPD patterns, whereas the low and moderate producers gave heterologous amplification patterns. Since Fusarium pallidoroseum was in another group, it was possible to distinguish between different species of the genus Fusarium by RAPD. These investigations may find an application in the diagnosis of unknown Fusarium species and in distinguishing isolates of Gibberella fujikuroi within the section of Liseola.
authors.affiliation: Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi.
authors.forename: Neena
authors.initials: N
authors.lastname: Mitter
authors.forename: A C
authors.initials: AC
authors.lastname: Srivastava
authors.lastname: Renu
authors.forename: Shahid
authors.initials: S
authors.lastname: Ahamad
authors.forename: A K
authors.initials: AK
authors.lastname: Sarbhoy
authors.forename: D K
authors.initials: DK
authors.lastname: Agarwal
mesh_terms: D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics*; D005670:Fusarium / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D014644:Genetic Variation; D005875:Gibberellins / Q000096:biosynthesis*; D016533:Mycological Typing Techniques; D010944:Plants / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D004271:DNA, Fungal; D005875:Gibberellins