ERK MAP Kinase is required in 1,25(OH)2D3-induced differentiation in human osteoblasts.
Expression of alkaline phosphatase(ALP)activity represents a key event during the differentiation processes of osteoblasts, and the level of ALP activity has been routinely used as a relative measure of differentiation stages of osteoblasts. In human osteoblasts, we showed that vitamin D3 analogue, 1,25(OH)2D3, had a stimulatory effect on ALP activity after 3 days, compared with control. The treatment of PD098059, an ERK MAP Kinase inhibitor, had a reducing effect on ALP activity, a differentiation marker in 1,25(OH)2D3-treated primary human osteoblasts. However, SB203580, a potent p38 MAP Kinase inhibitor, had no effect on the differentiation in this system. This indicates that ERK, not p38, is directly related to 1,25(OH)2D3-stimulated ALP activity in primary human osteoblasts. These results also show that the vitamin D3 analogue stimulates ERK1 activation in primary human osteoblasts. This finding provides one of signaling pathways for differentiation in primary human osteoblasts.
Chae
H J
HJ
Department of Dental Pharmacology and Wonkwang Dental Research Institute, School of Dentistry, Wonkwang University, Iksan, Chonbuk, South Korea.
Jeong
B J
BJ
Ha
M S
MS
Lee
J K
JK
Byun
J O
JO
Jung
W Y
WY
Yun
Y G
YG
Lee
D G
DG
Oh
S H
SH
Chae
S W
SW
Kwak
Y G
YG
Kim
H H
HH
Lee
Z H
ZH
Kim
H R
HR
D000469:Alkaline Phosphatase / Q000378:metabolism; D002117:Calcitriol / Q000494:pharmacology*; D002120:Calcium Channel Agonists / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000201:enzymology*; D011233:Precipitin Tests; D011725:Pyridines / Q000494:pharmacology
D002120:Calcium Channel Agonists; D004791:Enzyme Inhibitors; D005419:Flavonoids; D007093:Imidazoles; D011725:Pyridines; D020928:Mitogen-Activated Protein Kinases; D000469:Alkaline Phosphatase; D002117:Calcitriol; C093642:SB 203580; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one