title: Enzymes immobilized on magnetic carriers: efficient and selective system for protein modification.
abstract: In order to obtain an economical, efficient and selective system for glycoprotein modification we prepared reactors with immobilized neuraminidase or (and) galactose oxidase. High storage and operational stability of the enzyme reactors was obtained by their immobilization through the carbohydrate parts of the enzyme molecules to hydrazide-modified supports. Magnetic and non-magnetic forms of bead cellulose and poly(HEMA-co-EDMA) microspheres were used for immobilization. These reactors can be used almost universally for the activation of ligands and for labelling of substances having a carbohydrate moiety.
authors.affiliation: Department of Biological and Biochemical Sciences, University of Pardubice, Czech Republic. zuzana.bilkova@curie.fr
authors.forename: Z
authors.initials: Z
authors.lastname: Bílková
authors.forename: M
authors.initials: M
authors.lastname: Slováková
authors.forename: D
authors.initials: D
authors.lastname: Horák
authors.forename: J
authors.initials: J
authors.lastname: Lenfeld
authors.forename: J
authors.initials: J
authors.lastname: Churácek
mesh_terms: D001665:Binding Sites; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D008280:Magnetics
chemical_list: D004800:Enzymes, Immobilized; D006023:Glycoproteins