[Effects of cytokines and umbilical cord blood preserved at 4 degrees C on the growth of CFU-GM and HPP-CFC].
We studied the effects of different cytokine combinations and umbilical cord blood preserved at 4 degrees C for 4 days on the growth of CFU-GM and HPP-CFC. The results showed that the growth of umbilical cord blood CFU-GM was markedly different when various combinations of cytokines were used. After 2 weeks culturing with IL-3 alone or plus GM-CSF, the number of umbilical cord blood CFU-GM was increased when compared with GM-CSF group; upon stimulation with GM-CSF plus IL-3 plus IL-6 plus Epo, a significant increase of umbilical cord blood CFU-GM was seen. After umbilical cord blood being preserved 4 days at 4 degrees C, the number of umbilical cord blood CFU-GM and HPP-CFC was decreased.
Tan
Y
Y
Department of Blood Physiology, Hunan Medical University, Changsha 410078.
Li
W
W
Jiang
D
D
D001793:Blood Preservation / Q000379:methods; D003080:Cold Temperature; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology; D011247:Pregnancy
D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D015850:Interleukin-6