title: 5-Halogenated thymidine analogues induce a senescence-like phenomenon in HeLa cells.
abstract: We tested various thymidine analogues for induction of a senescence-like phenomenon in HeLa cells. CldU, BrdU, and IdU similarly induced the morphology of senescent cells and typical senescence markers. Thymidine analogues other than 5-halogenated forms caused only cell death. BrdU efficiently killed the cells in cooperation with irradiation with light and a brief treatment with Hoechst 33258, but CldU did not at all. 5-Halogenated thymidine analogues were thus shown to be specific inducers of cellular senescence in mammalian cells.
authors.affiliation: Division of Biochemistry, Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Yokohama, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Michishita
authors.forename: Naoto
authors.initials: N
authors.lastname: Matsumura
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kurahashi
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Suzuki
authors.forename: Hideki
authors.initials: H
authors.lastname: Ogino
authors.forename: Michihiko
authors.initials: M
authors.lastname: Fujii
authors.forename: Dai
authors.initials: D
authors.lastname: Ayusawa
mesh_terms: D001973:Bromodeoxyuridine / Q000494:pharmacology; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D010782:Photolysis; D013329:Structure-Activity Relationship; D013936:Thymidine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D001616:beta-Galactosidase; D001973:Bromodeoxyuridine; D013936:Thymidine