title: Detection of a variant metallo-beta-lactamase, IMP-10, from two unrelated strains of Pseudomonas aeruginosa and an alcaligenes xylosoxidans strain.
abstract: The gene bla(IMP-10) of a variant metallo-beta-lactamase, IMP-10, had a single base replacement of G by T at nucleotide 145, which led to an amino acid alteration of Val49 to Phe compared to the IMP-1 enzyme, indicating that IMP-10 was a point mutation derivative of IMP-1. Highly purified enzymes revealed that IMP-10 was different from IMP-1 in its extremely low hydrolyzing activities for penicillins, such as benzylpenicillin, ampicillin, and piperacillin.
authors.affiliation: Laboratory of Drug Resistance in Bacteria, Gunma University School of Medicine, Maebashi, Japan. siyobe@med.gunma-u.ac.jp
authors.forename: Shizuko
authors.initials: S
authors.lastname: Iyobe
authors.forename: Haruko
authors.initials: H
authors.lastname: Kusadokoro
authors.forename: Ayako
authors.initials: A
authors.lastname: Takahashi
authors.forename: Sachie
authors.initials: S
authors.lastname: Yomoda
authors.forename: Toyoji
authors.initials: T
authors.lastname: Okubo
authors.forename: Akio
authors.initials: A
authors.lastname: Nakamura
authors.forename: Koji
authors.initials: K
authors.lastname: O'Hara
mesh_terms: D000421:Alcaligenes / Q000201:enzymology*; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D015378:Imipenem / Q000494:pharmacology; D007700:Kinetics; D010957:Plasmids / Q000235:genetics; D017354:Point Mutation / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet; D013845:Thienamycins / Q000494:pharmacology; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D013845:Thienamycins; D015378:Imipenem; C466680:metallo-beta-lactamase, IMP-10; D001618:beta-Lactamases