A heat-sensitive TRP channel expressed in keratinocytes.
Mechanical and thermal cues stimulate a specialized group of sensory neurons that terminate in the skin. Three members of the transient receptor potential (TRP) family of channels are expressed in subsets of these neurons and are activated at distinct physiological temperatures. Here, we describe the cloning and characterization of a novel thermosensitive TRP channel. TRPV3 has a unique threshold: It is activated at innocuous (warm) temperatures and shows an increased response at noxious temperatures. TRPV3 is specifically expressed in keratinocytes; hence, skin cells are capable of detecting heat via molecules similar to those in heat-sensing neurons.
Peier
Andrea M
AM
Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA.
Reeve
Alison J
AJ
Andersson
David A
DA
Moqrich
Aziz
A
Earley
Taryn J
TJ
Hergarden
Anne C
AC
Story
Gina M
GM
Colley
Sian
S
Hogenesch
John B
JB
McIntyre
Peter
P
Bevan
Stuart
S
Patapoutian
Ardem
A
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D016466:CHO Cells; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000494:pharmacology; D027682:Cation Transport Proteins; D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006224:Cricetinae; D000078404:Epidermal Cells; D004817:Epidermis / Q000294:innervation / Q000378:metabolism; D005727:Ganglia, Spinal / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D017403:In Situ Hybridization; D007473:Ion Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism*; D008564:Membrane Potentials; D051379:Mice; D008969:Molecular Sequence Data; D009411:Nerve Endings / Q000502:physiology; D009474:Neurons / Q000502:physiology; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012430:Ruthenium Red / Q000494:pharmacology; D015398:Signal Transduction; D013116:Spinal Cord / Q000378:metabolism; D050916:TRPV Cation Channels; D013696:Temperature
D027682:Cation Transport Proteins; D007473:Ion Channels; D012333:RNA, Messenger; D050916:TRPV Cation Channels; C459445:TRPV3 protein, human; C486760:Trpv3 protein, mouse; D012430:Ruthenium Red; C071423:capsazepine; D002211:Capsaicin