[Experimental study on the induced-differentiation of human esophageal cancer cells treated with dimethylsulfoxide].
The fact that treatment of leukemia (Acute Promyelocytic Leukemia) with ATRA (All-Trans Retinoic Acid) was so succeeded that it was considered as a good example for tumor therapy. In the treatment of solid tumors by means of induced differentiation, however, has not been yet so broken-through. DMSO (Dimethylsulfoxide) was a common and simple organic compound, which comprised a variety of biological activities. For example, DMSO induced differentiation of leukemia in many reports. However, the effect of DMSO on solid tumors was to be explored further. In the present study, DMSO was used to human esophageal cancer cell lines in vitro in comparison with the classical inducer ATRA. From the view of morphology, cell cycle, growth inhibition, cytokeratin 4 expression, dye transfer and tumorigenecity, the results demonstrated that DMSO as well as ATRA could induce differentiation of human esophageal cancer cells. Interestingly, DMSO was confirmed to be more effective in inducing differentiation of esophageal cancer cells than ATRA. It suggests that DMSO showed some good prospects for the treatment of solid tumors.
Tan
L J
LJ
Dept. of Cell Biology, Shanghai Second Medical University, Shanghai 200025.
Tang
X M
XM
Shen
Z Y
ZY
Xu
J J
JJ
D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004938:Esophageal Neoplasms / Q000473:pathology*; D006801:Humans; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D014212:Tretinoin; D004121:Dimethyl Sulfoxide