Highly-substrate active isoenzyme acetylcholinesterase-II, in rosy eye mutant of Aedes aegypti mosquito.
Insecticide bioassays were carried out on larvae and adults of rosy eye mutant and wildtype strains of A. aegypti. Both the strains were equally susceptible to DDT, malathion and deltamethrin. Biochemical assays showed an increase in acetylcholinesterase enzyme (AChE) activity in all the stages of mutant strain with both the substrates i.e. acetylthiocholine iodide and S-butyrylthiocholine iodide. However, there was no difference in the percent inhibition of enzyme activity with propoxur in these two strains. Polyacrylamide gel electrophoresis performed in native conditions on the homogenates of adults of rosy eye mosquitoes showed that AChE-II allele was highly active with the substrate acetylthiocholine iodide as compared to wildtype strain. Frequency of the highly active AChE-II allele in the mutant strain was about 68%, whereas it was about 5% in the wildtype strain.
Mourya
D T
DT
National Institute of Virology, Pune, India. icmrniv@icmrniv.ren.nic.in
Gokhale
M D
MD
Barde
P V
PV
Deobagkar
D N
DN
D000110:Acetylcholinesterase / Q000378:metabolism*; D000330:Aedes / Q000201:enzymology* / Q000235:genetics; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000378:metabolism*; D009154:Mutation; D013379:Substrate Specificity
D002800:Cholinesterase Inhibitors; D007527:Isoenzymes; D000110:Acetylcholinesterase