title: From combinatorial chemistry to chemical microarray.
abstract: Combinatorial chemistry was first applied to the generation of peptide arrays in 1984. Since then, the field of combinatorial chemistry has evolved rapidly into a new discipline. There is a great need for the development of methods to examine the proteome functionally at a global level. Using many of the techniques and instruments developed for DNA microarrays, chemical microarray methods have advanced significantly in the past three years. High-density chemical microarrays can now be synthesized in situ on glass slides or be printed through covalent linkage or non-specific adsorption to the surface of the solid-support with fully automatic arrayers. Microfabrication methods enable one to generate arrays of microsensors at the end of optical fibers or arrays of microwells on a flat surface. In conjunction with the one-bead one-compound combinatorial library method, chemical microarrays have proven to be very useful in lead identification and optimization. High-throughput protein expression systems, robust high-density protein, peptide and small-molecule microarray systems, and automatic mass spectrometers are critical tools for the field of functional proteomics.
authors.affiliation: Division of Hematology and Oncology, UCD Cancer Center, 4501 X Street, Sacramento CA 95817, USA. kit.lam@ucdmc.ucdavis.edu
authors.forename: Kit S
authors.initials: KS
authors.lastname: Lam
authors.forename: Manat
authors.initials: M
authors.lastname: Renil
mesh_terms: D020650:Combinatorial Chemistry Techniques / Q000379:methods*