Overrepresentation of the short arm of chromosome 12 in seminoma and nonseminoma groups of testicular germ cell tumors.
The amplification of the short arm of the chromosome 12, especially as the i(12p) marker chromosome, has been found to be a highly nonrandom chromosome abnormality associated with testicular germ cell tumors (TGCT). A series of adult TGCT consisting of seven seminomas (SE) and eight nonseminomas (NS) was analyzed by conventional cytogenetics and fluorescent in situ hybridization. Multiplied chromosome 12 material originating from typical i(12p) and from other markers carrying chromosome 12-derived material was found in almost all analyzed tumors (6 of 7 SE cases and 8 of 8 NS cases). Heterogeneity in the copy number of i(12p) and other 12p-derived markers, as well as chromosome 12 aneuploidy, were higher in NS tumors than in SE.
Pienkowska-Grela
Barbara
B
Cytogenetic Laboratory, The Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, 5 K.W. Roentgen Str, 02-781, Warsaw, Poland. barpien@coi.waw.pl
Grygalewicz
Beata
B
Bregula
Urszula
U
D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D018239:Seminoma / Q000235:genetics*; D013736:Testicular Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured