A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein.
A rice spotted leaf (lesion-mimic) gene, Spl7, was identified by map-based cloning. High-resolution mapping with cleaved amplified polymorphic sequence markers enabled us to define a genomic region of 3 kb as a candidate for Spl7. We found one ORF that showed high similarity to a heat stress transcription factor (HSF). Transgenic analysis verified the function of the candidate gene for Spl7: leaf spot development was suppressed in spl7 mutants with a wild-type Spl7 transgene. Thus, we conclude that Spl7 encodes the HSF protein. The transcript of spl7 was observed in mutant plants. The levels of mRNAs (Spl7 in wild type and spl7 in mutant) increased under heat stress. Sequence analysis revealed only one base substitution in the HSF DNA-binding domain of the mutant allele, causing a change from tryptophan to cysteine.
Yamanouchi
Utako
U
Institute of the Society for Techno-Innovation of Agriculture, Forestry, and Fisheries, Tsukuba, Ibaraki 305-0854, Japan.
Yano
Masahiro
M
Lin
Hongxuan
H
Ashikari
Motoyuki
M
Yamada
Kyoji
K
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D005819:Genetic Markers; D006360:Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009687:Nuclear Proteins / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
D029681:Arabidopsis Proteins; D005819:Genetic Markers; D006360:Heat-Shock Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins; D012097:Repressor Proteins; C416221:SPL protein, Arabidopsis; D014157:Transcription Factors