Determination of grepafloxacin and clinafloxacin by capillary zone electrophoresis.
A simple and rapid capillary zone electrophoresis determination method with UV detection of grepafloxacin and clinafloxacin has been developed. The separation was performed in 35 mM borate-35 mM phosphate buffer solution (pH 8.6), containing 6% (v/v) of acetonitrile. Analyses were realised using fused-silica capillaries (57 cm length x 75 microm I.D.) and the operating conditions were: 15 kV applied voltage, 30 degrees C and detection at 279 nm. Piromidic acid was used as an internal standard. The linear concentration range of application was 1.0-120.0 microg ml(-1) for both compounds, with a detection limit of 0.2 microg ml(-1) for grepafloxacin and 0.3 microg ml(-1) for clinafloxacin. The analysis yielded good reproducibility (RSD between 3.37 and 1.74%). It was applied to the determination of grepafloxacin and clinafloxacin in human and rat urine samples. The method was validated using HPLC as a reference method. Recovery levels were between 94.5 and 103%.
Navalón
Alberto
A
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, E-18071 Granada, Spain.
Araujo
Lilia
L
Prieto
Avismelsi
A
Vílchez
José Luis
JL
D000818:Animals; D000890:Anti-Infective Agents / Q000652:urine*; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D024841:Fluoroquinolones; D006801:Humans; D010879:Piperazines / Q000652:urine*; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D010879:Piperazines; C057477:clinafloxacin; C075375:grepafloxacin