Surface expression of toll-like receptor 4 on THP-1 cells is modulated by Bu-Zhong-Yi-Qi-Tang and Shi-Quan-Da-Bu-Tang.
Human Toll-like receptor 4 (TLR4) has recently been identified and has been shown to be the main protein involved in recognizing Gram-negative bacteria. We examined the regulation of TLR4 surface expression in a human monocytic cell line (THP-1 cells) by two traditional Chinese herbal medicines. Bu-Zhong-Yi-Qi-Tang (TJ-41) and Shi-Quan-Da-Bu-Tang (TJ-48). TJ-41 and TJ-48 upregulated TLR4 surface expression in THP-1 cells, as well as enhanced TLR4 surface expression in these cells both dose- and time-dependently. These findings suggest that TJ-41 and TJ-48 increase the receptor involved in the response to Gram-negative bacteria and may enhance defenses against these pathogens.
Mita
Y
Y
First Department of Internal Medicine, Gunma University Faculty of Medicine, Gunma, Japan. ymita@pop.showa.gunma-u.ac.jp
Dobashi
K
K
Shimizu
Y
Y
Nakazawa
T
T
Mori
M
M
D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D029721:Drosophila Proteins; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D013997:Time Factors; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; D015854:Up-Regulation
D029721:Drosophila Proteins; D004365:Drugs, Chinese Herbal; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors