title: Calcium-dependent protection from complement lysis in Naegleria fowleri amebae.
abstract: Pathogenic Naegleria fowleri amebae are resistant to the lytic effects of serum complement. The presence of surface glycoproteins or removal of the membrane attack complex (MAC) of complement from the cell surface by vesiculation serve to protect the amebae from complement lysis. The specific mediators important in stimulating complement resistance are not defined. These studies were undertaken to examine the effect of Ca(2+) ions in initiating complement resistance of N. fowleri in contrast to non-pathogenic complement-sensitive N. gruberi. Chelation of extracellular calcium with ethylene glycol tetraacetic acid (EGTA) or chelation of intracellular calcium with 1,2-bis-(O-Aminophenoxy) ethane-N,N,N,N tetraacetic acid tetra (acetoxymethyl) ester (BAPTA-AM) increased complement lysis of N. fowleri. Chelation of calcium ions did not affect complement sensitivity of N. gruberi. Increased lysis of ionomycin-treated N. fowleri was detected after exposure to serum complement, suggesting that a threshold level of Ca(2+) mediates complement resistance before survival mechanisms are overwhelmed and lysis occurs. A differential influx of Ca(2+) ions occurred in fura-2 labeled N. fowleri after deposition of complement component C9 to form the MAC complex on the cell surface in comparison to N. gruberi. These studies suggest that Ca(2+) ions influence complement resistance in N. fowleri but do not play a role in altering the sensitivity of N. gruberi to complement.
authors.affiliation: Department of Microbiology and Immunology, VA Commonwealth University, Richmond, VA23298-0678, USA.
authors.forename: D-M
authors.initials: DM
authors.lastname: Chu
authors.forename: J
authors.initials: J
authors.lastname: Woodward
authors.forename: A
authors.initials: A
authors.lastname: Fritzinger
authors.forename: F
authors.initials: F
authors.lastname: Marciano-Cabral
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002614:Chelating Agents / Q000378:metabolism; D003167:Complement Activation; D003165:Complement System Proteins / Q000276:immunology / Q000378:metabolism*; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D016257:Fura-2 / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D017136:Ion Transport; D015759:Ionomycin / Q000378:metabolism; D007476:Ionophores / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D016848:Naegleria fowleri / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D019284:Thapsigargin / Q000378:metabolism
chemical_list: D002614:Chelating Agents; D005456:Fluorescent Dyes; D007476:Ionophores; C049925:fura-2-am; D008345:Manganese; D004533:Egtazic Acid; D015759:Ionomycin; D019284:Thapsigargin; D003165:Complement System Proteins; D008274:Magnesium; D002118:Calcium; D016257:Fura-2