title: Effect of abdominal infections on peritoneal and systemic production of interleukin 6 and monocyte chemoattractant protein-1.
abstract: The aim of the study was to compare peritoneal and systemic production of interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) in uninfected patients and in patients with peritonitis. Peritoneum was excised at laparotomy for acute peritonitis (n = 22) or noninfectious reasons (n = 61), and was incubated with or without lipopolysaccharide (LPS). Mediator concentrations in the culture-supernatants, in the patients' serum, and in plasmasupernatants of LPS-stimulated whole blood were related to outcome. Spontaneous production of IL-6 by the peritoneum was increased in infected patients compared with uninfected patients. In contrast to IL-6, LPS-stimulated production of MCP-1 was significantly less in infected patients. Serum concentrations of both mediators were higher in infected patients and the highest concentrations of MCP-1 were in patients who died. LPS-stimulated production of IL-6 in whole blood was least, whereas that of MCP-1 was greatest in infected patients who died. These contrasting results for local and systemic production of mediators illustrate the compartmentalized immune response to intra-abdominal infection.
authors.affiliation: Department of Surgery, University of Erlangen-Nuremberg, Germany.
authors.forename: Jutta
authors.initials: J
authors.lastname: Riese
authors.forename: Sven
authors.initials: S
authors.lastname: Schoolmann
authors.forename: Christian
authors.initials: C
authors.lastname: Denzel
authors.forename: Olaf
authors.initials: O
authors.lastname: Herrmann
authors.forename: Werner
authors.initials: W
authors.lastname: Hohenberger
authors.forename: Werner
authors.initials: W
authors.lastname: Haupt
mesh_terms: D001769:Blood / Q000378:metabolism; D018932:Chemokine CCL2 / Q000097:blood*; D005260:Female; D006801:Humans; D007239:Infections / Q000276:immunology / Q000378:metabolism*; D015850:Interleukin-6 / Q000097:blood*; D008070:Lipopolysaccharides; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D010537:Peritoneum / Q000378:metabolism; D010538:Peritonitis / Q000276:immunology / Q000378:metabolism* / Q000601:surgery; D015996:Survival Rate; D016896:Treatment Outcome
chemical_list: D018932:Chemokine CCL2; D015850:Interleukin-6; D008070:Lipopolysaccharides