title: Separable putative polyadenylation and cleavage motifs in Trichomonas vaginalis mRNAs.
abstract: 3' Untranslated region processing and polyadenylation in Trichomonas vaginalis was analyzed by 3' rapid amplification of cDNA ends and sequence analysis of T. vaginalis mRNAs. A putative polyadenylation signal with the sequence UAAA was found 11-30 nucleotides upstream from the cleavage site. The motif pyrimidine( downward arrow)(A)(0-3)AAUU is proposed to be the cleavage site for polyadenylation of transcripts. This potential sequence defining the cleavage site for polyadenylation in eukaryotes is a novel finding. As in other eukaryotes, runs of several U's downstream from the cleavage site were identified. A working hypothesis is proposed which couples the UAA translation stop codon with the signaling for the 3'end processing of transcripts in this early divergent parasitic protozoa.
authors.affiliation: Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70-228, 04510 Mexico D. F., Mexico.
authors.forename: Norma
authors.initials: N
authors.lastname: Espinosa
authors.forename: Roberto
authors.initials: R
authors.lastname: Hernández
authors.forename: Lorena
authors.initials: L
authors.lastname: López-Griego
authors.forename: Imelda
authors.initials: I
authors.lastname: López-Villaseñor
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics / Q000378:metabolism; D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011061:Poly A / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013389:Succinate-CoA Ligases / Q000235:genetics; D014158:Transcription, Genetic; D014246:Trichomonas vaginalis / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D000199:Actins; D012333:RNA, Messenger; D011061:Poly A; D013389:Succinate-CoA Ligases