Comparison of static and dynamic medium environments for culturing of pre-implantation mouse embryos.
The ability to manipulate mouse pre-implantation embryos in vitro has become a valuable tool in many scientific disciplines. However, fewer embryos maintain viability following in vitro manipulation compared with embryos in vivo. It has been suggested that use of dynamic medium environments may improve viability by simulating in utero environment. The objective of the study reported here was to compare a microdrop in vitro culture system with a microdevice in vitro culture system containing a static and two dynamic medium environments (0.1 and 0.5 microl/h) for culturing of mouse pre-implantation embryos. Results indicated that the static medium environment, in silicon-glass microdevices, was not significantly different from the microdrop control environment in proportion of embryos developing from the two-cell to the blastocyst stage. However, the static microdevice environment produced significantly (P < 0.05) more morulas than did that of the control group. Both of these treatment groups, under the presented conditions, consistently had significantly higher proportions of blastocysts (P < 0.05) and morulas (P < 0.05) and lower proportions of abnormal (P < 0.05) and eight-cell embryos (P < 0.05), compared with those of the high flow rate dynamic environment microdevice treatment groups. Studies exploring slower or pulsatile rates of medium delivery in a dynamic medium environment are indicated.
Hickman
Debra L
DL
Department of Animal Sciences, University of Illinois, Urbana 61801, USA.
Beebe
David J
DJ
Rodriguez-Zas
Sandra L
SL
Wheeler
Matthew B
MB
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry*; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains
D003470:Culture Media