Purine composition of the crystalline cytoplasmic inclusions of Paramecium tetraurelia.
Crystalline cytoplasmic inclusions were isolated by differential centrifugation from mass cultures of Paramecium tetraurelia feeding on Klebsiella pneumonia. Physical and chemical measurements of intact and solubilized crystals determined that they consist primarily of guanine and hypoxanthine with traces of xanthine. Crystals from the mutant sombre consist primarily of xanthine, suggesting there is a disorder of purine metabolism in this mutant.
Creutz
Carl E
CE
Department of Pharmacology, University of Virginia, Charlottesville 22908, USA. Creutz@virginia.edu
Mohanty
Sarina
S
Defalco
Tony
T
Kretsinger
Robert H
RH
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006147:Guanine / Q000032:analysis*; D007042:Hypoxanthines / Q000032:analysis*; D002479:Inclusion Bodies / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009154:Mutation; D016805:Paramecium tetraurelia / Q000737:chemistry* / Q000254:growth & development / Q000502:physiology; D014961:X-Ray Diffraction
D007042:Hypoxanthines; D006147:Guanine