title: Subfunctionalization of duplicate mitf genes associated with differential degeneration of alternative exons in fish.
abstract: The microphthalmia-associated transcription factor (MITF) exists in at least four isoforms. These are generated in higher vertebrates using alternative 5' exons and promoters from a single gene. Two separate genes (mitf-m and mitf-b), however, are present in different teleost fish species including the poeciliid Xiphophorus, the pufferfishes Fugu rubripes and Tetraodon nigroviridis, and the zebrafish Danio rerio. Fish proteins MITF-m and MITF-b correspond at both the structural and the expression levels to one particular bird/mammalian MITF isoform. In the teleost lineage subfunctionalization of mitf genes after duplication at least 100 million years ago is associated with the degeneration of alternative exons and, probably, regulatory elements and promoters. For example, a remnant of the first exon specific for MITF-m is detected within the pufferfish gene encoding MITF-b. Retracing the evolutionary history of mitf genes in vertebrates uncovered the differential recruitment of new introns specific for either the teleost or the bird/mammalian lineage.
authors.affiliation: Department of Physiological Chemistry I, Biocenter (Theodor-Boveri Institute), University of Würzburg, D-97074 Würzburg, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Altschmied
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Delfgaauw
authors.forename: Brigitta
authors.initials: B
authors.lastname: Wilde
authors.forename: Jutta
authors.initials: J
authors.lastname: Duschl
authors.forename: Laurence
authors.initials: L
authors.lastname: Bouneau
authors.forename: Jean-Nicolas
authors.initials: JN
authors.lastname: Volff
authors.forename: Manfred
authors.initials: M
authors.lastname: Schartl
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D003532:Cyprinodontiformes / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D019143:Evolution, Molecular; D005091:Exons; D020440:Gene Duplication; D051739:Microphthalmia-Associated Transcription Factor; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D023721:Tetraodontiformes / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D051739:Microphthalmia-Associated Transcription Factor; D014157:Transcription Factors