Cancer/testis antigen CSAGE is concurrently expressed with MAGE in chondrosarcoma.
Differential display-polymerase chain reaction was used to compare gene expression between human chondrosarcoma cell lines and normal cartilage. A new gene, CSAGE, has been cloned and belongs to a gene family that includes the taxol resistance associated gene (TRAG)-3. CSAGE, like TRAG-3, does not confer resistance to taxol when transfected in vitro. Both genes have alternatively spliced variants. CSAGE and TRAG-3 are expressed in chondrosarcoma, melanoma, and cartilage and testis, but not in other normal tissues. TRAG-3 has been reported to be a cancer/testis antigen. Our results suggest that CSAGE belongs to the growing list of cancer/testis antigens as well. In all of the CSAGE positive samples, the melanoma antigen gene family was also expressed. This is the first report on the expression of cancer/testis antigens in chondrosarcoma.
Lin
Chuzhao
C
Department of Orthopaedics, Rhode Island Hospital, Brown University School of Medicine, Providence, RI, USA.
Mak
Solida
S
Meitner
Patricia A
PA
Wolf
Jennifer M
JM
Bluman
Eric M
EM
Block
Joel A
JA
Terek
Richard M
RM
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D002813:Chondrosarcoma / Q000235:genetics* / Q000473:pathology; D019008:Drug Resistance, Neoplasm; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020014:K562 Cells; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D017239:Paclitaxel / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000951:Antigens, Neoplasm; C457202:CSAG1 protein, human; C118809:CSAG2 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D017239:Paclitaxel