[Immunophenotyping of eighty six children with acute lymphoblastic leukemia by three-color flow cytometry].
OBJECTIVE
To evaluate the specificity of three-color flow cytometry in childhood acute lymphoblastic leukemia (ALL) immunophenotyping.


METHODS
Immunophenotyping was performed by three-color flow cytometry analysis using CD(45)/SSC gating.


RESULTS
The percentage of blasts was correlated better with leukemic cell count compared with that of FSC/SSC, and the false positive results were low. Among eighty six cases of ALL, 95.3% was B-ALL, in which common-ALL and Pro-B-ALL were 76.8% and 6.1%, respectively, and 2.3% was T-ALL. CD(34)(+) and myeloid-associated antigen expression were observed in 57.0% and 34.9% of the cases, respectively, among which Pro-B-ALL was the commonest. CD(33) was more commonly expressed than CD(13) in Pro-B-ALL cases, but no difference in the expression between these two antigens in other subtypes.


CONCLUSIONS
Gating of CD(45)/SSC eliminated effection of normal cells to blasts in bone marrow, with which the immunophenotyping results were more reliable.
Wan
Suigui
S
Department of Hematology, Xuanwu Hospital, CUMS, Beijing 100053, China.
Gong
Wenyu
W
Sun
Xuejing
X
Xu
Juan
J
Tian
Ding
D
D015703:Antigens, CD / Q000032:analysis; D002648:Child; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology*
D015703:Antigens, CD