[Establishment of sandwich ELISA with McAbs to measure histidine-rich glycoprotein in plasma].
The objective of this paper is to establish a method using sandwich ELISA with McAbs to measure plasma histidine-rich glycoprotein (HRG). The results showed that this method, with a working range from 2.5 ng.ml-1 to 80 ng.ml-1, provided the lower limit of detection of 1 ng.ml-1, the average intra-assay CV, the interassay CV and the recovery rate of HRG were 8.2%, 11.5% and 89.1%, respectively. Interference by antithrombin III and human albumin was not significant. With this method, the concentration of plasma HRG from healthy donors was (110.3 +/- 9.7) mg.L-1, and the value of plasma HRG in liver cirrhosis was (79.5 +/- 12.6) mg.L-1. These results suggest that sandwich ELISA with McAbs is high sensitive, precise and specific.
Xu
K
K
Department of Clinical Biochemistry, Hunan Medical University, Changsha 410013.
Chen
Z
Z
D000328:Adult; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood; D008297:Male; D011506:Proteins / Q000032:analysis*; D000099053:Histidine-Rich Glycoprotein
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D011506:Proteins; D000099053:Histidine-Rich Glycoprotein