title: Electron microscopic detection of tin accumulation in biliopancreatic concrements after induction of chronic pancreatitis in rats by di-n-butyltin dichloride.
abstract: The organotin compound di-n-butyltin dichloride (DBTC) is able to induce an acute and later a chronic pancreatitis in rats. In previous papers the authors demonstrated this DBTC pancreatitis as a rat model for an interstitial pancreatitis with tendency to transduction to the chronic form. DBTC is excreted according to its lipophilic nature by liver and bile. Therefore, the bilio-pancreatic main duct is necrotized by the tin-loaded bile. The duct system is blocked by cell debris and later by epithelial proliferations. In the chronic phase, numerous rats develop concrements in the main duct. In the present paper, the authors report about bacterial growth in some bilio-pancreatic concrements. Whereas the electron microscopic detection of tin by energy-dispersive X-ray analysis (EDX) in SEM or electron energy loss spectroscopy (EELS) in TEM was negative in the parenchyma of pancreas and liver, some concrements with bacterial cells were positive for this element. Tin mapping with energy spectroscopic imaging (ESI) in TEM demonstrated the congruency of tin signals and electron-dense particles inside these bacteria and of electron-dense accumulations in the matrix of these concrements. The low content of tin in pancreatic and liver tissue and the higher quantity of tin inside the bacterial contaminated concrements were supported by atomic absorption spectrophotometry (AAS). The paper discusses the long time preservation of tin in the concrements as an action of heavy-metal- accumulating bacteria, which should be classified in the future by bacteriological methods.
authors.affiliation: Electron Microscopic Centre, Department of Pathology, Medical Faculty, University of Rostock, Germany. ludwig.jonas@med.uni-rostock.de
authors.forename: Ludwig
authors.initials: L
authors.lastname: Jonas
authors.forename: Gerhard
authors.initials: G
authors.lastname: Fulda
authors.forename: Geofred
authors.initials: G
authors.lastname: Kröning
authors.forename: Jutta
authors.initials: J
authors.lastname: Merkord
authors.forename: Horst
authors.initials: H
authors.lastname: Nizze
mesh_terms: D000818:Animals; D001419:Bacteria / Q000648:ultrastructure; D002137:Calculi / Q000139:chemically induced / Q000737:chemistry / Q000382:microbiology / Q000648:ultrastructure*; D002908:Chronic Disease; D003135:Common Bile Duct / Q000187:drug effects / Q000648:ultrastructure*; D004195:Disease Models, Animal; D004577:Electron Probe Microanalysis; D007275:Injections, Intravenous; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D009947:Organotin Compounds / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity*; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010183:Pancreatic Ducts / Q000187:drug effects / Q000648:ultrastructure; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011917:Rats, Inbred Lew; D013054:Spectrophotometry, Atomic; D014001:Tin / Q000032:analysis / Q000378:metabolism*
chemical_list: D009947:Organotin Compounds; D014001:Tin; C020735:dibutyldichlorotin