title: Basement membrane and connective tissue proteins in intestinal mucosa of patients with coeliac disease.
abstract: OBJECTIVE
  Gluten ingestion in coeliac disease is associated with alterations of the intestinal mucosa, especially the expansion of the lamina propria. Antiendomysium and antireticulin antibodies may result from interactions between gliadin and extracellular matrix components. By behaving as autoantigens, connective tissue proteins could initiate mucosal damage. This study evaluates changes in the distribution of laminin, type IV collagen, and fibronectin in the mucosa of patients with coeliac disease in an attempt to explain the alterations of mucosal morphology.
  
  
  METHODS
  Intestinal biopsies were obtained from patients with coeliac disease on admission and while on a gluten free diet. The distribution of type IV collagen, laminin, fibronectin, and alpha-smooth muscle actin was evaluated by immunofluorescence and by immunogold labelling and electron microscopy.
  
  
  RESULTS
  In patients with coeliac disease, the intensity of type IV collagen, laminin, and fibronectin immunofluorescent staining was decreased and less well defined than in controls, with frequent breaches in the basement membrane; fibronectin staining was weak in the distal third of the elongated crypts and absent under the flat surface. The distribution of smooth muscle fibre in the distal lamina propria of flat mucosae was altered. The distribution of these proteins was normal as assessed by immunoelectron microscopy.
  
  
  CONCLUSIONS
  The intensity of staining of some components of the basement membrane is decreased in coeliac disease and the distribution of smooth muscle fibres is altered. These changes may result from interactions between gliadin and components of the extracellular matrix and may play a role in the genesis of mucosal lesions and in the damage to the epithelium.
authors.affiliation: Gastroenterology Unit, Human Nutrition Division, Institute of Nutrition and Food Technology, J.P. Alessandri 5540, Santiago, Chile.
authors.forename: S
authors.initials: S
authors.lastname: Verbeke
authors.forename: M
authors.initials: M
authors.lastname: Gotteland
authors.forename: M
authors.initials: M
authors.lastname: Fernández
authors.forename: J
authors.initials: J
authors.lastname: Bremer
authors.forename: G
authors.initials: G
authors.lastname: Ríos
authors.forename: O
authors.initials: O
authors.lastname: Brunser
mesh_terms: D001485:Basement Membrane / Q000378:metabolism; D002446:Celiac Disease / Q000178:diet therapy / Q000378:metabolism* / Q000473:pathology; D024141:Collagen Type IV / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005983:Glutens / Q000008:administration & dosage; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D016253:Microscopy, Immunoelectron
chemical_list: D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D005983:Glutens