title: The effects of sialic acid on the gene expression of fibrillar collagens: different changes in normal and fibrotic scar derived fibroblasts.
abstract: OBJECTIVE
  To investigate the effect of free sialic acid on collagen gene expression in fibroblasts.
  
  
  METHODS
  Cell culture study.
  
  
  METHODS
  University hospital, Finland. CELL LINES: Human granulation tissue fibroblasts, human hypertrophic scar fibroblasts and human keloid fibroblasts.
  
  
  METHODS
  Treatment of cell cultures with 3 microM, 30 microM and 300 microM N-acetyl-neuraminic acid.
  
  
  METHODS
  The measurement of steady state level of mRNA for type I and type III collagen.
  
  
  RESULTS
  Fibroblast lines react dissimilarly under the influence of sialic acid. Granulation tissue fibroblasts showed decrease in the gene expression of type I and III collagen, while keloid fibroblasts contrastingly showed an increase. Hypertrophic scar derived fibroblasts showed no change.
  
  
  CONCLUSIONS
  Sialic acids may decrease collagen gene expression in granulation tissue and that disturbed wound healing in diabetics and smokers may in part be due to direct effect of sialic acids on fibroblasts. Sialic acids may in part induce keloid formation.
authors.affiliation: Department of Surgery, University of Turku, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Kössi
authors.forename: J
authors.initials: J
authors.lastname: Aalto
authors.forename: S
authors.initials: S
authors.lastname: Haataja
authors.forename: J
authors.initials: J
authors.lastname: Niinikoski
authors.forename: J
authors.initials: J
authors.lastname: Peltonen
authors.forename: M
authors.initials: M
authors.lastname: Laato
mesh_terms: D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000235:genetics*; D024042:Collagen Type I / Q000187:drug effects / Q000235:genetics*; D024061:Collagen Type III / Q000187:drug effects / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects*; D006097:Granulation Tissue / Q000187:drug effects; D006801:Humans; D006824:Hybridization, Genetic; D019158:N-Acetylneuraminic Acid / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis*; D012016:Reference Values; D012680:Sensitivity and Specificity; D014945:Wound Healing / Q000502:physiology
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D012333:RNA, Messenger; D019158:N-Acetylneuraminic Acid