gp130-linked signal transduction promotes the differentiation and maturation of dendritic cells.
In order to explore the role of gp130-linked signal transduction in the differentiation and maturation of dendritic cells (DC), the mAb, B-S12, an agonist of gp130, was used for the activation of gp130 on DC. The effects of cytokines and of anti-gp130 mAb on the proliferation of DC, and their expression of IL-12 and CD80 (B7-1) by DC were evaluated. DC differentiating from peripheral blood mononuclear cells did not express the IL-6 receptor alpha chain, but expressed gp130. Anti-gp130 mAb promoted the proliferation of DC, induced by IL-4 and granulocyte macrophage colony stimulating factor (GM-CSF), by up-regulating the GM-CSF receptor on DC. DC induced by gp130 mAb and cytokines expressed DC-derived CC chemokine, as measured by RT-PCR. Induced DC also stimulated strong proliferation of autologous T cells in mixed lymphocyte reaction since an up-regulated expression of IL-12 and CD80 (B7-1) was observed in DC activated by anti-gp130 mAb. Thus, gp130 signal transduction is important for the differentiation and maturation of DC.
Wang
Yue-Dan
YD
Department of Immunology, Soochow (Suzhou) University, 48 Renmin Road, Suzhou, China 215007.
Gu
Zong-Jiang
ZJ
Huang
Jian-An
JA
Zhu
Yi-Bei
YB
Zhou
Zhao-Hua
ZH
Xie
Wei
W
Xu
Ying
Y
Qiu
Yu-Hua
YH
Zhang
Xue-Guang
XG
D000328:Adult; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000378:metabolism*; D002454:Cell Differentiation; D002455:Cell Division; D019742:Chemokines, CC / Q000235:genetics; D050822:Cytokine Receptor gp130; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D018664:Interleukin-12 / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C111792:CCL18 protein, human; D019742:Chemokines, CC; C494199:IL6ST protein, human; D008562:Membrane Glycoproteins; D050822:Cytokine Receptor gp130; D018664:Interleukin-12