title: Mineral status of embryos of domestic fowl following exposure in vivo to the carbonic anhydrase inhibitor acetazolamide.
abstract: Eggs of domestic fowl were given daily injections of vehicle (DMSO) or vehicle plus acetazolamide, a potent inhibitor of the enzyme carbonic anhydrase, beginning on day 12 of incubation. Embryos were removed from eggs on days 16 and 18, and carcasses and yolks were analyzed for calcium, magnesium, and phosphorus. Treatment with acetazolamide did not affect the quantity of calcium or phosphorus in carcasses and the effect, if any, on magnesium in carcasses was small. However, calcium content of yolk was reduced substantially by acetazolamide both on day 16 and day 18. The reduction in calcium content of yolk led, in turn, to a reduction in the total quantity of calcium in eggs on days 16 and 18. Embryos exposed to acetazolamide seemingly mobilized less calcium from the eggshell than did control embryos. When faced with a shortfall in the availability of calcium from the eggshell, embryos defended carcass calcium, and the shortfall was reflected in a reduction in the quantity of calcium deposited in yolk. The results of this study support the concept that the enzyme carbonic anhydrase plays a role in solubilization of the eggshell and provision of calcium to embryos.
authors.affiliation: Department of Biology, Colorado State University, Fort Collins, CO 80523-1878, USA. kpackard@lamar.colostate.edu
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Packard
authors.forename: Leslie D
authors.initials: LD
authors.lastname: Lohmiller
mesh_terms: D000086:Acetazolamide / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000032:analysis*; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology*; D002642:Chick Embryo; D004625:Embryo, Nonmammalian / Q000737:chemistry / Q000187:drug effects*; D008274:Magnesium / Q000032:analysis*; D010758:Phosphorus / Q000032:analysis*; D015053:Zygote / Q000737:chemistry / Q000187:drug effects
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D010758:Phosphorus; D008274:Magnesium; D000086:Acetazolamide; D002118:Calcium