title: 7,8-Dihydroneopterin induces apoptosis of Jurkat T-lymphocytes via a Bcl-2-sensitive pathway.
abstract: Activated cell-mediated immunity is known to be accompanied by elevated concentrations of 7,8-dihydroneopterin which in high concentrations was found to interfere with the oxidant-antioxidant balance. In this study we investigated whether 7,8-dihydroneopterin mediates apoptosis of Jurkat T-lymphocytes via a CrmA- or Bcl-2-sensitive pathway. Transient transfection assays with CrmA and Bcl-2 expression constructs showed that apoptosis was not affected by CrmA whereas it was significantly decreased upon cotransfection with Bcl-2 constructs. Results suggest that 7,8-dihydroneopterin-induced apoptosis of T-lymphocytes is mediated by a Bcl-2-sensitive pathway.
authors.affiliation: Institute for Medical Chemistry and Biochemistry, Innsbruck, Austria.
authors.forename: Christiane
authors.initials: C
authors.lastname: Enzinger
authors.forename: Barbara
authors.initials: B
authors.lastname: Wirleitner
authors.forename: Christina
authors.initials: C
authors.lastname: Lutz
authors.forename: Günther
authors.initials: G
authors.lastname: Böck
authors.forename: Bettina
authors.initials: B
authors.lastname: Tomaselli
authors.forename: Gottfried
authors.initials: G
authors.lastname: Baier
authors.forename: Dietmar
authors.initials: D
authors.lastname: Fuchs
authors.forename: Gabriele
authors.initials: G
authors.lastname: Baier-Bitterlich
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D019169:Jurkat Cells; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D019798:Neopterin / Q000031:analogs & derivatives; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D011621:Pteridines / Q000494:pharmacology*; D015843:Serpins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D019311:Staurosporine / Q000494:pharmacology; D014764:Viral Proteins
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D019253:Proto-Oncogene Proteins c-bcl-2; D011621:Pteridines; D015843:Serpins; D014764:Viral Proteins; C000976:7,8-dihydroneopterin; D019798:Neopterin; C074641:interleukin-1beta-converting enzyme inhibitor; D020928:Mitogen-Activated Protein Kinases; D019311:Staurosporine