title: Determination of lormetazepam and its main metabolite in serum using micellar electrokinetic capillary chromatography with direct injection and ultraviolet absorbance detection.
abstract: The use of micellar electrokinetic chromatography for the determination of lormetazepam and its metabolite, lorazepam, in serum samples at a concentration range of therapeutic interest was investigated. The separation was carried out at 30 degrees C and 25 kV, using a 15 mM borate-phosphate buffer (pH 8) with 30 mM sodium dodecyl sulfate as the separation electrolyte and 15% methanol as organic modifier. The analyses were carried out in 20 min under these conditions. Detection limits of 0.5 mg l(-1) were achieved for both benzodiazepines in serum. This method was employed for the quantitative resolution of both drugs (at different concentration ratios) in serum with very good recoveries.
authors.affiliation: Department of Analytical Chemistry and Food Technology, Faculty of Chemistry, University of Castilla-La Mancha, E-13071 Ciudad Real, Spain. jjberzas@qata-cr.uclm.es
authors.forename: J J
authors.initials: JJ
authors.lastname: Berzas Nevado
authors.forename: G
authors.initials: G
authors.lastname: Castañeda Peñalvo
authors.forename: M J
authors.initials: MJ
authors.lastname: Pinilla Calderón
mesh_terms: D014151:Anti-Anxiety Agents / Q000097:blood*; D001569:Benzodiazepines; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008140:Lorazepam / Q000031:analogs & derivatives* / Q000097:blood*; D012680:Sensitivity and Specificity; D013501:Surface-Active Agents
chemical_list: D014151:Anti-Anxiety Agents; D002021:Buffers; D013501:Surface-Active Agents; D001569:Benzodiazepines; C023842:lormetazepam; D008140:Lorazepam