title: The rgl-1 is a legitimate homologue of lethal giant larvae recessive oncogene in rat.
abstract: We have cloned a rat homologue of the Drosophila recessive oncogene lethal (2) giant larvae from rat brain by RT-PCR using primers prepared from sequences conserved amongst lgl family genes. Sequence analysis predicts that the rat rgl-1 gene encodes a 1,036 amino acid polypeptide with a molecular weight of approximately 112 kDa, which contains a domain characteristic of WD-40 proteins. Northern blot analysis revealed that the highest expression of rgl-1 is detected in the testis, with moderate expression in ovary, brain, spleen, and kidney. Since there is a high degree of amino acid similarity among lgl proteins in various species, it is likely that there is an evolutionary relationship among these proteins. The amino acid identity of rgl-1 to Drosophila l(2)gl and mouse mgl-1 proteins showed 30.6 and 96.8%, respectively. The rat tomosyn, previously known as a homologue of Drosophila l(2)gl, showed much lower amino acid identity to Drosophila l(2)gl and mouse mgl-1 proteins (17.8 and 20%, respectively). Functional analysis showed that the expression of a rat rgl-1 cDNA in Saccharomyces cerevisiae missing sop genes, the yeast homologues of the Drosophila l(2)gl, restored partially the Na+ tolerance of the cell. Taken together, these results indicate that rgl-1, not tomosyn, is the legitimate homologue of lgl gene.
authors.affiliation: College of Medicine, Pochon CHA University, CHA General Hospital, Seoul, Korea.
authors.forename: Yong-Soo
authors.initials: YS
authors.lastname: Kim
authors.forename: Kwang-Hyun
authors.initials: KH
authors.lastname: Baek
authors.forename: Kwang Youl
authors.initials: KY
authors.lastname: Lee
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Chung
authors.forename: Kyung-Ah
authors.initials: KA
authors.lastname: Lee
authors.forename: Jung Jae
authors.initials: JJ
authors.lastname: Ko
authors.forename: Kwang Yul
authors.initials: KY
authors.lastname: Cha
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016147:Genes, Tumor Suppressor; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats
chemical_list: D011506:Proteins; C457639:RGL1 protein, rat; D012333:RNA, Messenger