title: Fragmentation and dispersal of the pericentriolar Golgi complex is required for entry into mitosis in mammalian cells.
abstract: The pericentriolar Golgi stacks are fragmented and found dispersed in mitotic mammalian cells. Addition of an antibody to the Golgi-associated protein GRASP65 inhibited Golgi fragmentation by mitotic cytosol in permeabilized cells. Microinjecting this antibody or the C-terminal fragment of GRASP65, which contains the antibody binding site, into normal rat kidney cells prevented entry into mitosis. Under these conditions the cells had completed S phase but were not in the prophase stage of mitosis. Fragmentation of the Golgi apparatus by nocodazole or Brefeldin A treatment prior to or post microinjection of the anti-GRASP65 antibody alleviated the block in mitotic entry. Based on our findings, we suggest that the pericentriolar Golgi organization is a sensor for controlling entry into mitosis in mammalian cells.
authors.affiliation: Cell and Developmental Biology Department, Division of Biology, University of California, San Diego, La Jolla 92093, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Sütterlin
authors.forename: Pattie
authors.initials: P
authors.lastname: Hsu
authors.forename: Arrate
authors.initials: A
authors.lastname: Mallabiabarrena
authors.forename: Vivek
authors.initials: V
authors.lastname: Malhotra
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D001665:Binding Sites; D015415:Biomarkers; D020126:Brefeldin A / Q000494:pharmacology; D002478:Cells, Cultured; D002502:Centrioles / Q000378:metabolism / Q000502:physiology*; D000939:Epitopes / Q000378:metabolism; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D000077614:Golgi Matrix Proteins; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects / Q000502:physiology*; D015739:Nocodazole / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D015415:Biomarkers; D000939:Epitopes; D000077614:Golgi Matrix Proteins; C491577:Gorasp1 protein, rat; D008565:Membrane Proteins; D011994:Recombinant Proteins; D020126:Brefeldin A; D016590:Aphidicolin; D015739:Nocodazole