Fast determination of retinol and alpha-tocopherol in plasma by LC.
A fast, selective and economical method for the determination of retinol and alpha-tocopherol is presented. Both vitamins are separated by high-performance liquid chromatography (HPLC) in less than 4 min using an isocratic elution with methanol. The robustness of the method was checked in real samples, obtaining relative standard deviation lower than 3%. The described method was satisfactorily applied to serum samples proceeding of patients enrolled in a METHADONE maintenance treatment program. The retinol concentrations in the serum of these patients fell into the normal interval of concentrations; however, the serum alpha-tocopherol contents were higher than the normal values.
Rodríguez-Delgado
M A
MA
Department of Analytical Chemistry, Nutrition and Food Science, University of La Laguna, 38201, La Laguna, Spain. mrguez@ull.es
Díaz-Flores Estévez
J F
JF
Díaz-Flores Estévez
F
F
Hernández Calzadilla
C
C
Díaz Romero
C
C
D002851:Chromatography, High Pressure Liquid; D006556:Heroin Dependence / Q000097:blood / Q000534:rehabilitation; D006801:Humans; D007202:Indicators and Reagents; D008691:Methadone / Q000627:therapeutic use; D009294:Narcotics / Q000627:therapeutic use; D012015:Reference Standards; D012016:Reference Values; D014801:Vitamin A / Q000097:blood*; D014810:Vitamin E / Q000097:blood*
D007202:Indicators and Reagents; D009294:Narcotics; D014801:Vitamin A; D014810:Vitamin E; D008691:Methadone