title: Optimization of the separation of some Chelidonium maius L. alkaloids by reversed phase high-performance liquid chromatography using cyanopropyl bonded stationary phase.
abstract: The high-performance liquid chromatographic method using two stationary phases: RP-18 and cyanopropyl silica for the analysis of some alkaloids in Chelidonium maius L. extracts is performed. The extract was chromatographed using a mobile phase composed of an organic modifier (acetonitrile, methanol, tetrahydrofuran or 1,4-dioxan), phosphate buffer and sodium octadecylsulfate or di (2-ethyl hexyl) orthophosphoric acid (HDEHP) as ion-pairing reagents. The influence of the different kind of stationary phase and organic modifiers on retention has been compared. In order to improve the selectivity of separation and peak shape, gradient elution was applied.
authors.affiliation: Department of Inorganic and Analytical Chemistry, Medical University, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Petruczynik
authors.forename: Maria
authors.initials: M
authors.lastname: Gadzikowska
authors.forename: Monika
authors.initials: M
authors.lastname: Waksmundzka-Hajnos
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry; D028281:Chelidonium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D018514:Plant Structures / Q000737:chemistry; D012821:Silanes / Q000737:chemistry
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D012821:Silanes; C097372:cyanopropyl dimethyl dimethylamino silane