5-Halogenated thymidine analogues induce a senescence-like phenomenon in HeLa cells.
We tested various thymidine analogues for induction of a senescence-like phenomenon in HeLa cells. CldU, BrdU, and IdU similarly induced the morphology of senescent cells and typical senescence markers. Thymidine analogues other than 5-halogenated forms caused only cell death. BrdU efficiently killed the cells in cooperation with irradiation with light and a brief treatment with Hoechst 33258, but CldU did not at all. 5-Halogenated thymidine analogues were thus shown to be specific inducers of cellular senescence in mammalian cells.
Michishita
Eriko
E
Division of Biochemistry, Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Yokohama, Japan.
Matsumura
Naoto
N
Kurahashi
Toshihiro
T
Suzuki
Toshikazu
T
Ogino
Hideki
H
Fujii
Michihiko
M
Ayusawa
Dai
D
D001973:Bromodeoxyuridine / Q000494:pharmacology; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D010782:Photolysis; D013329:Structure-Activity Relationship; D013936:Thymidine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000378:metabolism
D001616:beta-Galactosidase; D001973:Bromodeoxyuridine; D013936:Thymidine