Abnormal accumulation in lipopolysaccharide in biliary epithelial cells of rats with self-filling blind loop.
Primary sclerosing cholangitis (PSC) is known to be frequently associated with inflammatory bowel diseases. In a rat with self-filling blind loop (SFBL), a proposed animal model for PSC, hepatobiliary inflammation has previously been demonstrated. In this study, we assessed the involvement of lipopolysaccharide (LPS), a bacterial endotoxin, in the pathogenesis of hepatobiliary inflammation of the SFBL model. The hepatic localization of LPS was examined by immunohistochemistry using an anti-lipid A antibody. The portal blood concentration of LPS was measured by an endotoxin-specific chromogenic Limulus test (Endospecy test). LPS was localized in the biliary epithelial cells (BECs) of rats with SFBL, and the portal blood concentration of LPS was significantly higher than that of sham-operated rats. Development of hepatobiliary inflammation, peribiliary fibrosis, and injury to the intestinal mucosa were histologically confirmed. Constriction in the biliary trees was radiologically demonstrated. These findings suggested that abnormal accumulation of LPS, which may be derived from portal blood, in BECs was involved in the pathogenesis of hepatobiliary inflammation with intestinal injury.
Koga
Hironori
H
Second Department of Medicine, Kurume University School of Medicine, 830-0011, Japan. hirokoga@med.kurume-u.ac.jp
Sakisaka
Shotaro
S
Yoshitake
Masao
M
Harada
Masaru
M
Kumemura
Hiroto
H
Hanada
Shinichiro
S
Taniguchi
Eitaro
E
Kawaguchi
Takumi
T
Kumashiro
Ryukichi
R
Sata
Michio
M
D000818:Animals; D001652:Bile Ducts / Q000033:anatomy & histology* / Q000473:pathology*; D002758:Cholangiography; D004847:Epithelial Cells / Q000378:metabolism*; D007150:Immunohistochemistry; D007249:Inflammation; D008050:Lipid A / Q000276:immunology; D008070:Lipopolysaccharides / Q000097:blood / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D008050:Lipid A; D008070:Lipopolysaccharides