title: The E-selectin SER128ARG gene polymorphism and restenosis after successful coronary angioplasty.
abstract: OBJECTIVE
  Coronary angioplasty remains plagued by the problem of restenosis. Genetic polymorphisms may contribute to the development of restenosis by mediating exaggerated inflammatory responses of the endothelium to angioplasty-induced injury.
  
  
  BACKGROUND
  The serine (Ser)-128-arginine (Arg) gene polymorphism of E-selectin has been implicated in the pathogenesis of coronary artery disease (CAD). We sought to explore whether allelic variants relate to post-angioplasty restenosis.
  
  
  METHODS
  The 128Arg allele was analyzed by PCR in 101 (derivation study, age 54+/-1 years, all mean+/-S.E.M.) and 92 (validation study, age 62+/-1 years) patients with CAD who underwent successful angioplasty.
  
  
  RESULTS
  Restenosis, defined as >50% luminal diameter reduction at the target lesion at follow-up angiography, was found in 54/101 (53%) and 43/92 (47%) patients during follow-up. The 128Arg allele frequency in the derivation study was 10.39% and was 11.96% in the validation study. The 128Arg allele was more prevalent in the restenosis groups (14.81% and 17.44%, respectively) than in the restenosis-free groups (5.32% and 7.14%, respectively, p=0.027 and p=0.031). In multivariate logistic regression, the 128Arg allele emerged as a predictor of restenosis in both studies (p<0.05). There were no differences in the level of soluble E-selectin according to genotype, gender, age (p>0.20), and between patients with restenosis and those without (43.8+/-3.2 vs. 47.4+/-3.1 ng/ml, p>0.20).
  
  
  CONCLUSIONS
  The 128Arg allele of E-selectin may be related to increased endothelial responses to injury, thereby potentially serving as a risk factor for post-angioplasty restenosis in patients with CAD.The development of restenosis remains a problem in patients with CAD. The Ser128Arg polymorphism of E-selectin was analyzed in 101 (derivation) and 92 (validation) CAD patients. Patients with restenosis (54/101 and 43/92) had a higher frequency of the 128Arg allele (14.81 and 17.44%) than those without (5.32%, p=0.027 and 7.14%, p=0.031). In logistic regression, the 128Arg allele emerged as a predictor of restenosis in both studies (p<0.05). The E-selectin 128Arg allele may serve as a risk factor for the development of restenosis.
authors.affiliation: Klinik und Poliklinik für Innere Medizin III, Martin-Luther-Universität Halle-Wittenberg, Ernst-Grube-Strasse 40, D-06097 Halle/Saale, Germany. mathias.rauchhaus@medizin.uni-halle.de
authors.forename: Mathias
authors.initials: M
authors.lastname: Rauchhaus
authors.forename: Michael
authors.initials: M
authors.lastname: Gross
authors.forename: Susanne
authors.initials: S
authors.lastname: Schulz
authors.forename: Darrel P
authors.initials: DP
authors.lastname: Francis
authors.forename: Petra
authors.initials: P
authors.lastname: Greiser
authors.forename: Antje
authors.initials: A
authors.lastname: Norwig
authors.forename: Lorenz
authors.initials: L
authors.lastname: Weidhase
authors.forename: Andrew J S
authors.initials: AJ
authors.lastname: Coats
authors.forename: Rainer
authors.initials: R
authors.lastname: Dietz
authors.forename: Stefan D
authors.initials: SD
authors.lastname: Anker
authors.forename: Christiane
authors.initials: C
authors.lastname: Gläser
mesh_terms: D015906:Angioplasty, Balloon, Coronary / Q000009:adverse effects*; D001120:Arginine / Q000235:genetics; D017023:Coronary Angiography; D003327:Coronary Disease / Q000235:genetics* / Q000628:therapy; D023903:Coronary Restenosis / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D011237:Predictive Value of Tests; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics*; D012694:Serine / Q000235:genetics; D012795:Sialoglycoproteins; D016896:Treatment Outcome
chemical_list: D008562:Membrane Glycoproteins; D017468:Receptors, Fibroblast Growth Factor; D012795:Sialoglycoproteins; C086711:cysteine-rich fibroblast growth factor receptor; D012694:Serine; D001120:Arginine