title: Morphological changes in immunopositive cells of ionotropic glutamate receptor subunits during the development of transplanted fetal ventral mesencephalic neurons.
abstract: To elucidate the morphological changes in immunopositive cells of ionotropic glutamate receptors within intrastriatal 'developing' grafts of fetal ventral mesencephalon (VM) in 6-hydroxydopamine-lesioned rats, immunohistochemistry was performed to detect cells expressing N-methyl-D-aspartate (NMDA) receptor subunit 1 (NR1), the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunits (GluR1, GluR2/3, and GluR4), or tyrosine hydroxylase (TH) in the intrastriatal VM grafts at 1, 4, and 12 weeks following transplantation. One week after transplantation, TH-positive cells were detected without any immunoreactivity of the NMDA and AMPA receptor subunits in the grafts. Four weeks after transplantation, TH-positive cells, distributed homogeneously in the grafts, appeared to be multipolar and larger compared to those at 1 week post-grafting. At this stage, we could observe immunopositive cells of NMDA and AMPA receptors distributed homogeneously in the grafts. Twelve weeks after transplantation, the numbers of NR1- and GluR1-positive cells were smaller than that at 4 weeks post-grafting, whereas TH-positive cells appeared to be more matured in shape and size. On the other hand, the numbers of GluR2/3- and GluR4-positive cells were not changed as compared with those at 4 weeks post-grafting. These results suggest that the ionotropic glutamate receptors have differential roles during the developmental period of the intrastriatal VM grafts.
authors.affiliation: Department of Psychiatry, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki-gun, 889-1692, Miyazaki, Japan. ishiday@post.miyazaki-med.ac.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ishida
authors.forename: Kazunari
authors.initials: K
authors.lastname: Todaka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hashiguchi
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Takeda
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Mitsuyama
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Nishimori
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D016380:Brain Tissue Transplantation; D002454:Cell Differentiation; D004195:Disease Models, Animal; D005260:Female; D016332:Fetal Tissue Transplantation; D006085:Graft Survival; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000166:cytology / Q000637:transplantation*; D008694:Methamphetamine / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation*; D016627:Oxidopamine; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000096:biosynthesis*; D016194:Receptors, N-Methyl-D-Aspartate / Q000096:biosynthesis; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis
chemical_list: C409466:NR1 NMDA receptor; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D008694:Methamphetamine; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase