Galactosaminoglycan composition in chicken eggshell.
Galactosaminoglycans, isolated from decalcified chicken eggshell by papain digestion and ion-exchange chromatography, were fractionated by selective precipitation at varying concentrations of ethanol and characterized by chemical and enzymatic methods. The eggshell contained 0.15 microg galactosaminoglycan uronic acid/mg dry weight. Most (to approximately 87% of total) galactosaminoglycans were found to be chondroitin sulfate-dermatan sulfate copolymers with iduronic acid contents being approximately 20 to 30% of uronic acid. The remaining (to approximately 12% of total) galactosaminoglycans were chondroitin sulfate-dermatan sulfate copolymers with higher iduronic acid contents averaging 59% of uronic acid. Results of chondroitinase-ABC digestion demonstrated 4-sulfated disaccharides to be the major repeating units in the chicken eggshell galactosaminoglycans.
Nakano
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T
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada. tnakano@ualberta.ca
Ikawa
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N
Ozimek
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L
D000818:Animals; D005591:Chemical Fractionation; D002645:Chickens; D002809:Chondroitin Sulfates / Q000032:analysis; D002852:Chromatography, Ion Exchange / Q000379:methods / Q000662:veterinary; D003871:Dermatan Sulfate / Q000031:analogs & derivatives* / Q000032:analysis; D004528:Egg Shell / Q000737:chemistry*; D004588:Electrophoresis, Cellulose Acetate / Q000662:veterinary; D000431:Ethanol / Q000737:chemistry; D006820:Hyaluronic Acid / Q000032:analysis; D007067:Iduronic Acid / Q000032:analysis; D010206:Papain / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis*; D014574:Uronic Acids / Q000032:analysis
D011134:Polysaccharides; D014574:Uronic Acids; C069524:dermatan sulfate chondroitin sulfate; D003871:Dermatan Sulfate; D007067:Iduronic Acid; D000431:Ethanol; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; C007815:galactosaminoglycan; D010206:Papain