title: Characterization and expression of two genes encoding isoforms of a putative Na, K-ATPase in the chytridiomycete Blastocladiella emersonii.
abstract: A P-type ATPase gene (BePAT1) from the aquatic fungus Blastocladiella emersonii, which surprisingly showed high similarity with the alpha-subunit of Na, K-ATPases from animal cells, has been reported recently [Biochim. Biophys. Acta 1383 (1998) 183]. In the present study, we describe the characterization of a second gene, denominated BePAT2, and show that these two genes have a different intron-exon structure but encode putative proteins with greater than 90% amino acid identity. Northern blot and multiplex reverse transcription and polymerase chain reaction (RT-PCR) assays have revealed that BePAT1 and BePAT2 genes have a non-coordinate, developmentally regulated expression during B. emersonii life cycle. Phosphoenzyme formation experiments using the immunopurified enzymes have indicated the presence of a Na, K-ATPase-like activity. Furthermore, immunofluorescence studies using B. emersonii zoospores localized the ATPases on the plasma membrane of these cells.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748-São Paulo-SP 05508-900, Brazil.
authors.forename: Luciano Gomes
authors.initials: LG
authors.lastname: Fietto
authors.forename: Luciana
authors.initials: L
authors.lastname: Pugliese
authors.forename: Suely Lopes
authors.initials: SL
authors.lastname: Gomes
mesh_terms: D001483:Base Sequence; D001754:Blastocladiella / Q000235:genetics*; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000302:isolation & purification; D019143:Evolution, Molecular; D005800:Genes, Fungal; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011233:Precipitin Tests; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D013172:Spores, Fungal; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D000254:Sodium-Potassium-Exchanging ATPase