Embryonic lethality of mutant mice deficient in the p116 gene.
We report a lethal phenotype of mouse embryo with a disruption in the gene encoding p116, a subunit of the translation initiation factor, eIF3. The amino acid sequence of mouse p116, as deduced from the cDNA, shows high homology (97%) with human p116, and contains the conserved RNA binding sites, RNP1 and RNP2. The p116 mRNA is ubiquitously expressed in various organs, suggesting a house-keeping function of the p116 protein. To obtain genetic evidence for the essential role of the p116 protein in mouse cells, we constructed mice with a disruption in the p116 gene. Heterozygous p116(+/-) mice were intercrossed, and the genotypes of the offspring were determined. The results indicated no p116(-/-) pups among 84 neonates. Also, there were no p116(-/-) embryos 13.5 days postcoitum (d.p.c.). Among 77 embryos, there was only one p116(-/-) embryo at the blastocyst stage (3.5 d.p.c.). These results indicate that p116 plays an essential role in the early stages of mouse development.
Koyanagi-Katsuta
Rimiko
R
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
Akimitsu
Nobuyoshi
N
Hamamoto
Hiroshi
H
Arimitsu
Nagisa
N
Hatano
Toshiyuki
T
Sekimizu
Kazuhisa
K
D000595:Amino Acid Sequence; D000818:Animals; D018387:Codon, Initiator; D018076:DNA, Complementary / Q000032:analysis; D004622:Embryo, Mammalian / Q000473:pathology*; D039621:Eukaryotic Initiation Factor-3 / Q000172:deficiency / Q000235:genetics*; D016678:Genome; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000172:deficiency / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D018387:Codon, Initiator; D018076:DNA, Complementary; D039621:Eukaryotic Initiation Factor-3; D021122:Protein Subunits