Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype.
We sought to test the hypothesis that dietary long-chain n-3 PUFA (LC n-3 PUFA) in fish oil stimulate the gene expression of lipoprotein lipase (LPL) in human adipose tissue (AT). In a randomized, double blind, placebo-controlled, cross-over study, 51 male subjects expressing an atherogenic lipoprotein phenotype (ALP) had their diets supplemented with fish oil for 6 weeks. As we previously reported for this group, supplementation with LC n-3 PUFA produced a decrease in fasting plasma triglyceride (TG) (-35%, P < 0.05), attenuation of the postprandial TG response (area and incremental area under the curve; AUC and IAUC, P < 0.05), and a decrease in small, dense LDL. The present study extended these observations by showing that these changes were accompanied by a marked increase in the concentration of LPL mRNA in adipose tissue (AT-LPL mRNA, +55%, P = 0.003) and post-heparin LPL activity (PH-LPL, +31%, P = 0.036). There was also evidence of an association between LPL gene expression and polymorphism in the apolipoprotein E gene. We conclude that the favorable influence of dietary n-3 PUFA on the ALP may be mediated, in part, through an increase in the plasma activity and gene expression of lipoprotein lipase in human adipose tissue.
Khan
Syrah
S
School of Biomedical & Life Sciences, University of Surrey, Guildford, United Kingdom.
Minihane
Anne-Marie
AM
Talmud
Philippa J
PJ
Wright
John W
JW
Murphy
Margaret C
MC
Williams
Christine M
CM
Griffin
Bruce A
BA
D000273:Adipose Tissue / Q000378:metabolism*; D001057:Apolipoproteins E / Q000235:genetics; D001792:Blood Platelets / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D018592:Cross-Over Studies; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D004311:Double-Blind Method; D005227:Fatty Acids / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005395:Fish Oils / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008071:Lipoprotein Lipase / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D001057:Apolipoproteins E; D004041:Dietary Fats; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D008055:Lipids; D008074:Lipoproteins; D012333:RNA, Messenger; D008071:Lipoprotein Lipase