title: Impact of Heavy Metals on the Selective Phenotypical Markers of Pseudomonas aeruginosa.
abstract: The aim of this study was to characterize the impact of heavy metals on phenotypical markers of Pseudomonas aeruginosa. Twenty-two isolates of P. aeruginosa, either clinical (20) or secondary treated wastewater (2), were used to inoculate micro-ecosystems of sterile distilled water or secondary waste effluent in the presence of subminimal inhibitory concentrations of a variety of heavy metals commonly encountered in the aquatic naturally habitat (Ca2+, Co2+, Cr3+, Cu2+, Hg2+, Ni2+, Zn2+). Micro-ecosystems were exposed to visible light at laboratory temperature and individual strains were reisolated after a 1-, 3-, or 6-month period. The re-isolates (129) were characterized using hierarchical classification analysis in order to define affinities among variants of P. aeruginosa. Subsequently, discriminant analysis was used to detect eventual relationships among the different phenotypical markers studied. Results of the hierarchical classification, based on qualitative or quantitative approaches, showed clearly that incubation of P. aeruginosa in the presence of heavy metals altered the studied phenotypical markers, namely serotype, phage type, MIC of metals, and pyocin type. Discriminant analysis showed that the studied phenotypical markers could be classified into four clusters: C1 (L1 and L2 phage types, Hg tolerance and/or resistance, S2 serotype), C2 (P2 pyocin type, Cd tolerance and/or resistance, S1 serotype), C3 (Co and Cr tolerance and/or resistance) and C4 (P1 pyocin type, Ni, Zn, and Cu tolerance and/or resistance).
authors.affiliation: Laboratoire Eau et Environnement, Institut National de Recherche Scientifique et Technique, BP 95 - 2050, Hammam-Lif, Tunisia.
authors.forename: A.
authors.initials: A
authors.lastname: Hassen
authors.forename: Z.
authors.initials: Z
authors.lastname: Jerboui
authors.forename: M.
authors.initials: M
authors.lastname: Chérif
authors.forename: N.
authors.initials: N
authors.lastname: Saidi
authors.forename: S.
authors.initials: S
authors.lastname: Gharbi
authors.forename: A.
authors.initials: A
authors.lastname: Boudabous