Differential modulation of host macrophage function by AK-5 tumor is dependent on the site of tumor transplantation.
We have studied the mechanisms involved in the activation or suppression of the immune response by the tumor cells, which is also dependent on the site of tumor transplantation. AK-5 tumor cells when transplanted s.c. activated peritoneal macrophages through the induction of different cytokines, whereas tumor cells when transplanted i.p. suppressed the activity of macrophages as well as the production of cytokines. Suppression of macrophage function was also observed when they were cocultured with tumor cells in vitro. Our studies suggest the capability of AK-5 tumor in driving the immune response either towards activated status or towards immune suppression, depending on the site of transplantation. Activated macrophages produced free radicals, which were cytotoxic to the tumor cells.
Mitra
Roshni
R
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Bhaumik
Sraboni
S
Khar
Ashok
A
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology*; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D030421:Peroxynitrous Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
C113948:AK-5 antigen; D000951:Antigens, Neoplasm; D016207:Cytokines; D017382:Reactive Oxygen Species; D030421:Peroxynitrous Acid; C002744:3-nitrotyrosine; D014443:Tyrosine; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat