title: Distraction osteogenesis enhanced by osteoblastlike cells and collagen gel.
abstract: Femoral distraction was done in rats to determine whether the injection of osteoblastlike cells with collagen gel into the distracted callus was useful for new bone formation. The cells were obtained from the femoral marrow of Sprague-Dawley rats and cultured for approximately 3 weeks. These rats were divided into four groups. The rats in Group A received injections of physiologic saline, those in Group B received injections of collagen gel, those in Group C received injections of cells, and those in Group D received injections containing a mixture of cells and collagen gel. The distracted areas were harvested and evaluated by histologic analysis, radiography, three-point bending testing, and the weight of femoral ash. Histologic evaluation did not show an immunoreaction between the donor and recipient. Radiographs showed that Group D had the most callus, and the fracture strength in this group as determined by the three-point bending test was higher than in Group A at 2, 4, and 6 weeks after elongation was completed. Group D showed a significant difference in the ash weight of the distracted femurs at 2 weeks. The current study showed that osteoblastlike cells with collagen gel promoted new bone formation in the distracted gap, and shortened the consolidation period.
authors.affiliation: Department of Orthopaedic Surgery, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takamine
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Takahiko
authors.initials: T
authors.lastname: Kitakoji
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kurita
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ono
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Ohshima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitoh
authors.forename: Naoki
authors.initials: N
authors.lastname: Ishiguro
authors.forename: Hisashi
authors.initials: H
authors.lastname: Iwata
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001707:Biopsy, Needle; D001861:Bone Regeneration / Q000502:physiology*; D017690:Cell Transplantation; D003094:Collagen / Q000494:pharmacology*; D004195:Disease Models, Animal; D005269:Femur / Q000473:pathology* / Q000601:surgery*; D017102:Fracture Healing / Q000502:physiology; D008297:Male; D010006:Osteoblasts / Q000637:transplantation*; D019857:Osteogenesis, Distraction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013718:Tensile Strength
chemical_list: D003094:Collagen