The redox state of plastoquinone pool regulates state transitions via cytochrome b6f complex in Synechocystis sp. PCC 6803.
The effects of benzoquinone analogues, phenyl-1,4-benzoquinone (PBQ) and 2,5-dibromo-3-methyl-6-isopropyl-1,4-benzoquinone (DBMIB), on state transitions in Synechocystis sp. PCC 6803 were investigated. PBQ induced a transition from state 2 to state 1 in the absence of actinic light whereas DBMIB caused a state 2 transition. 3-(3,4-Dichlorophenyl)-1,1-dimethyl urea could not eliminate the effects of PBQ and DBMIB. These results imply that the redox state of the plastoquinone pool controls the state transitions in vivo and cytochrome b6f complex is involved in this process. As a working hypothesis, we propose that the occupancy of the quinol oxidation site and the movement of the Rieske protein may be pivotal in this regulation.
Mao
Hai Bin
HB
State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, PR China.
Li
Guo Fu
GF
Ruan
Xiang
X
Wu
Qing Yu
QY
Gong
Yan Dao
YD
Zhang
Xiu Fang
XF
Zhao
Nan Ming
NM
D016227:Benzoquinones / Q000494:pharmacology; D000458:Cyanobacteria / Q000378:metabolism*; D003573:Cytochrome b Group / Q000378:metabolism*; D045346:Cytochrome b6f Complex; D003991:Dibromothymoquinone / Q000494:pharmacology; D008027:Light; D009097:Multienzyme Complexes / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010971:Plastoquinone / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D016227:Benzoquinones; D003573:Cytochrome b Group; D009097:Multienzyme Complexes; D003991:Dibromothymoquinone; C029322:phenylbenzoquinone; D045346:Cytochrome b6f Complex; D010971:Plastoquinone