Cloning and characterization of a phospholipase C from the C(4) plant Digitaria sanguinalis.
As a PLC activity was implicated in the light transduction pathway that controls C(4) photosynthesis in Digitaria sanguinalis, a full length PLC cDNA (DsPLC2) was cloned. The proteins encoded by the two possible open reading frames were produced in Escherichia coli; they both harbour a PLC activity but with different response to Ca(2+) concentration, and with different sensitivity to the PLC inhibitor U-73122.
Coursol
Sylvie
S
Institut de Biotechnologie des Plantes, UMR 8618, Université Paris XI, Bâtiment 630, 91405 Orsay Cedex, France.
Pierre
Jean-Noël
JN
Vidal
Jean
J
Grisvard
Jeanine
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D004963:Estrenes / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D006109:Poaceae / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D011760:Pyrrolidinones / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D010738:Type C Phospholipases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D004963:Estrenes; D011760:Pyrrolidinones; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D010738:Type C Phospholipases; D002118:Calcium