Infiltrating CD14+ monocytes and expression of CD14 by activated parenchymal microglia/macrophages contribute to the pool of CD14+ cells in ischemic brain lesions.
CD14, a key pattern recognition receptor of the innate immune system, is a surface molecule on monocytic cells involved in cellular activation. We investigated 18 autopsy cases of focal cerebral infarctions (FCI) by immunohistochemistry to examine CD14 expression following ischemia. Controls confirmed constitutive CD14 expression by few perivascular cells. In contrast to quiescent CD14- parenchymal microglial cells, following ischemia activated microglia/macrophages expressed abundant CD14. In FCI, CD14+ cells increased both in perivascular spaces and in brain parenchyma within 1-2.5 days and remained elevated until late stages. Early CD14 expression suggests an essential part of CD14 in the acute inflammatory response following stroke.
Beschorner
Rudi
R
Institute of Brain Research, University of Tübingen Medical School, Calwer Str. 3, Germany. rudi.beschorner@med.uni-tuebingen.de
Schluesener
Hermann J
HJ
Gözalan
Fatma
F
Meyermann
Richard
R
Schwab
Jan M
JM
D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies; D001921:Brain / Q000276:immunology / Q000473:pathology; D002545:Brain Ischemia / Q000276:immunology* / Q000473:pathology* / Q000503:physiopathology; D002196:Capillaries / Q000166:cytology; D004660:Encephalitis / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000032:analysis* / Q000096:biosynthesis; D008264:Macrophages / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D008297:Male; D017628:Microglia / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D020521:Stroke / Q000276:immunology / Q000473:pathology / Q000503:physiopathology
D000906:Antibodies; D018950:Lipopolysaccharide Receptors