title: Production of polyclonal antibodies to Batrachochytrium dendrobatidis and their use in an immunoperoxidase test for chytridiomycosis in amphibians.
abstract: Polyclonal antibodies were produced for diagnosing chytridiomycosis in amphibians. Two sheep and 4 rabbits were inoculated with homogenized whole culture of Batrachochytrium dendrobatidis in Freund's complete adjuvant or triple adjuvant. Antisera from all animals reacted strongly with all stages of B. dendrobatidis and stained the walls, cytoplasm, rhizoids and zoospores in an indirect immunoperoxidase test. Significant cross-reactivity occurred only with some fungi in the Chytridiomycota, and there are no members of this phylum besides B. dendrobatidis that infect frogs. The immunoperoxidase stain is a useful screening test when combined with recognition of the morphology and infection site of B. dendrobatidis.
authors.affiliation: Australian Animal Health Laboratory, CSIRO Livestock Industries, Geelong, Victoria.
authors.forename: Lee
authors.initials: L
authors.lastname: Berger
authors.forename: Alex D
authors.initials: AD
authors.lastname: Hyatt
authors.forename: Veronica
authors.initials: V
authors.lastname: Olsen
authors.forename: Sandra G
authors.initials: SG
authors.lastname: Hengstberger
authors.forename: Donna
authors.initials: D
authors.lastname: Boyle
authors.forename: Gerry
authors.initials: G
authors.lastname: Marantelli
authors.forename: Kaye
authors.initials: K
authors.lastname: Humphreys
authors.forename: Joyce E
authors.initials: JE
authors.lastname: Longcore
mesh_terms: D000663:Amphibians / Q000382:microbiology*; D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D000946:Antigens, Fungal / Q000032:analysis*; D008411:Chytridiomycota / Q000276:immunology*; D003429:Cross Reactions; D003881:Dermatomycoses / Q000175:diagnosis / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D007114:Immunization / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary
chemical_list: D000908:Antibodies, Fungal; D000946:Antigens, Fungal