title: Hydrolytic properties of crude alpha-L-rhamnosidases produced by several wild strains of mesophilic fungi.
abstract: OBJECTIVE
  Observation of the dependence of alpha-L-rhamnosidase activity on pH and temperature and the capability to hydrolyse concentrated naringin solutions and hesperidin suspensions of enzyme complexes produced by several fungi.
  
  
  RESULTS
  The enzymes were produced by several wild strains of mesophilic fungi grown in liquid media containing rhamnose as sole carbon source. The properties and their ranges of values measured were as follows: (i) optimum pH, 3.5-6.5; (ii) optimum temperature, 50-65 degrees C; (iii) hydrolysis of supersaturated 100 g l(-1) naringin solutions, 45-100% and (iv) hydrolysis of hesperidin suspensions, 6-35%.
  
  
  CONCLUSIONS
  Some alpha-L-rhamnosidase enzymes hydrolysed supersaturated naringin solutions with a high yield. The enzyme produced by Fusarium sambucinum 310 showed good activity even at pH 10.
  
  
  CONCLUSIONS
  Crude enzymes with possible utilization as catalysts for the manufacture of hydrolysis products of the flavonoid glycosides were found.
authors.affiliation: Instituto de Investigaciones para la Industria Química, Universidad Nacional de Salta, CONICET, Buenos Aires, Salta, Argentina.
authors.forename: E
authors.initials: E
authors.lastname: Scaroni
authors.forename: C
authors.initials: C
authors.lastname: Cuevas
authors.forename: L
authors.initials: L
authors.lastname: Carrillo
authors.forename: G
authors.initials: G
authors.lastname: Ellenrieder
mesh_terms: D001230:Aspergillus / Q000201:enzymology; D044950:Flavanones; D005419:Flavonoids / Q000378:metabolism*; D005670:Fusarium / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006569:Hesperidin / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009089:Mucor / Q000201:enzymology; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology
chemical_list: D044950:Flavanones; D005419:Flavonoids; D006569:Hesperidin; D006026:Glycoside Hydrolases; C022525:alpha-L-rhamnosidase; C005274:naringin