Effects of acylation on the structure, lipid binding, and transfer activity of wheat lipid transfer protein.
Study of the effect of protein chemical acylation on their functional properties or activity often brings valuable information regarding structure-function relationships. We performed such work on wheat lipid transfer protein, LTP1, to investigate the role of grafted acyl chains on the lipid binding and transfer properties. LTP1 was acylated by using anhydride derivatives of various chain lengths from C2 to C6. Only the chemical modifications with hexanoic acid yielded a marked effect on the tertiary structure and a slight change in the secondary structure. The affinity of the modified proteins for myristoyl-lysophosphatidylcholine was similar to that of the native protein accompanied by a slight decrease in stoichiometry. Interestingly, the acylation of LTP1 enhanced the lipid transfer activity by at least a factor of 10 for hexanoic chain length. Finally, the grafting of acyl chains was investigated by means of molecular modelling, and an attempt is made to correlate with our experimental data.
Pato
Christine
C
Unité de Biochimie et Technologie des Protéines, Institut National de la Recherche Agronomique, Nantes, France.
Tran
Vinh
V
Marion
Didier
D
Douliez
Jean Paul
JP
D000215:Acylation; D000812:Anhydrides / Q000737:chemistry; D052179:Antigens, Plant; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D002942:Circular Dichroism; D007700:Kinetics; D008024:Ligands; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D008958:Models, Molecular; D010940:Plant Proteins; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D017186:Titrimetry / Q000379:methods; D014908:Triticum / Q000737:chemistry* / Q000378:metabolism*; D014443:Tyrosine / Q000737:chemistry
D000812:Anhydrides; D052179:Antigens, Plant; D002352:Carrier Proteins; D008024:Ligands; D008055:Lipids; D010940:Plant Proteins; C511920:lipid transfer proteins, plant; D014443:Tyrosine