Technetium-99m labeling anti-amastigote polyclonal antibodies of Leishmania amazonensis.
Anti-amastigote polyclonal antibody (IgG) was incubated with solutions of stannous chloride and sodium borohidride. After that, 3.7 MBq of technetium-99m (99mTc) was added. A labeling yield of the antibody about 84% was obtained. After filtration of 99mTc-IgG, the radiochemical purity increased from 84 to 95%. The labeling of IgG with 99mTc did not modify the immunoreactivity of the antibody, since it was able to identify in vitro and in vivo the specific antigen of Leishmania amazonensis.
Araujo
J G V C
JG
Laboratório de Radioisótopos, Faculdade de Farmácia, UFMG, Brazil.
Toledo
V P C P
VP
Guimarães
T M P D
TM
Bernardo-Filho
M
M
Simal
C J R
CJ
Mota
L G
LG
Diniz
S O F
SO
Cardoso
V N
VN
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D006224:Cricetinae; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007553:Isotope Labeling / Q000379:methods; D007894:Leishmania mexicana / Q000302:isolation & purification*; D007897:Leishmaniasis, Mucocutaneous / Q000000981:diagnostic imaging* / Q000276:immunology / Q000378:metabolism; D009666:Nose / Q000000981:diagnostic imaging / Q000276:immunology; D016719:Radioimmunodetection / Q000379:methods; D019275:Radiopharmaceuticals; D012016:Reference Values; D012680:Sensitivity and Specificity; D013667:Technetium
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D019275:Radiopharmaceuticals; D013667:Technetium