title: Comparative study of two methods for RNA extraction prior to detection of resistance to human immunodeficiency virus type 1 with the line probe assay.
abstract: We evaluated two methods from Roche and Promega for RNA extraction prior to the genotypic detection of human immunodeficiency virus type 1 resistance by line probe assay (LiPA). Fifty plasma RNA extracts were processed in parallel by LiPA. Results obtained by the Roche method were superior in the proportion of amplified samples, the percentage of mutated samples, and band intensity.
authors.affiliation: Microbiology Service, University Hospital of Valladolid, Spain. eiros@med.uva.es
authors.forename: Jose María
authors.initials: JM
authors.lastname: Eiros
authors.forename: Cristina
authors.initials: C
authors.lastname: Labayru
authors.forename: Beatriz
authors.initials: B
authors.lastname: Hernández
authors.forename: Raúl
authors.initials: R
authors.lastname: Ortiz De Lejarazu
authors.forename: Antonio
authors.initials: A
authors.lastname: Rodríguez Torres
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology* / Q000627:therapeutic use; D024882:Drug Resistance, Viral; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D016333:HIV Protease / Q000235:genetics; D054303:HIV Reverse Transcriptase / Q000235:genetics; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D015336:Molecular Probe Techniques; D012367:RNA, Viral / Q000302:isolation & purification*
chemical_list: D019380:Anti-HIV Agents; D012367:RNA, Viral; D054303:HIV Reverse Transcriptase; D016333:HIV Protease