title: Calcium salts deposition in rat connective tissue after the implantation of calcium hydroxide-containing sealers.
abstract: This study was conducted to observe the rat subcutaneous connective tissue reaction to implanted dentin tubes that were filled with mineral trioxide aggregate, Sealapex, Calciobiotic Root Canal Sealer (CRCS), Sealer 26, and the experimental material, Sealer Plus. The animals were sacrificed after 7 and 30 days, and the specimens were prepared for histological analysis after serial sections with a hard-tissue microtome. The undecalcified sections were examined with polarized light after staining according to the Von Kossa technique for calcium. At the tube openings, there were Von Kossa-positive granules that were birefringent to polarized light. Next to these granulations, there was irregular tissue, like a bridge, that was Von Kossa-positive. The dentin walls of the tubes exhibited a structure highly birefringent to polarized light, usually like a layer, in the tubules. These results were observed with all the studied materials, except the CRCS, which didn't exhibit any kind of mineralized structure. The results suggest that among the materials studied, the CRCS could have the least possibility of encouraging hard tissue deposition.
authors.affiliation: Department of Endodontics, Faculdade de Odontologia de Araçatuba, UNESP, São Paulo, Brazil.
authors.forename: Roberto
authors.initials: R
authors.lastname: Holland
authors.forename: Valdir
authors.initials: V
authors.lastname: de Souza
authors.forename: Mauro Juvenal
authors.initials: MJ
authors.lastname: Nery
authors.forename: o Felicio Estrada
authors.initials: oF
authors.lastname: Bernabé
authors.forename: José Arlindo Otoboni
authors.initials: JA
authors.lastname: Filho
authors.forename: Eloi Dezan
authors.initials: ED
authors.lastname: Junior
authors.forename: Sueli Satomi
authors.initials: SS
authors.lastname: Murata
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D001718:Birefringence; D001729:Bismuth / Q000494:pharmacology; D017610:Calcium Compounds / Q000032:analysis* / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000494:pharmacology*; D003238:Connective Tissue / Q000737:chemistry / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure; D003809:Dentin, Secondary / Q000737:chemistry; D004338:Drug Combinations; D006801:Humans; D010087:Oxides / Q000494:pharmacology; D051381:Rats; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012459:Salicylates / Q000494:pharmacology; D017640:Silicates / Q000494:pharmacology; D015034:Zinc Oxide / Q000494:pharmacology
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D012459:Salicylates; C080620:Sealer 26; D017640:Silicates; C086631:mineral trioxide aggregate; C055601:Sealapex; C075563:Calcibiotic Root Canal Sealer; D002126:Calcium Hydroxide; D015034:Zinc Oxide; D001729:Bismuth