[Configuration in solution and activity mechanism studies on the anticancer Fe(II)-pingyangmycin (PYM) complex].
In order to elucidate the micro-configuration and mechanism of anticancer activity of Fe(II)-PYM complex in solution, the coordination of high spin paramagnetic ferrous ion, Fe(II), and Pingyangmycin, (PYM) was studied in D2O solution by using the paramagnetic ion probe principle and PMR technique and compared with the inactive complexes, e.g., Cu(II)-PYM and Fe(III)-PYM. The basic configuration parameter of the complexes were determined. When the pyrimidine methyl proton was close to the central ion, 6.5 x 10(-10) m was taken as standard, the absolute distance between the central ion and other protons or ligand could be calculated. Results showed that, in Fe(III) solution, the proton of pyrimidine methyl and the terminal acyl group of the sugar moiety were closer to the central ion, but other protons were relaxed and the distance was between 7.0 x 10(-10) m and 15 x 10(-10) m. In the case of Fe(II) complex, the distance of the central ion and the ligand group was between 4.91 x 10(-10) m and 8.85 x 10(-10) m. The distinctive structure diversity with those of inactive complexes was observed and the relationship of the activity mechanism was postulated.
Jiang
J
J
Microbiology Institute, Shanxi Academy of Sciences, Xi'an 710043.
Guo
Y
Y
Zhou
X
X
D000903:Antibiotics, Antineoplastic / Q000737:chemistry* / Q000494:pharmacology; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology; D005296:Ferrous Compounds / Q000737:chemistry* / Q000494:pharmacology; D008968:Molecular Conformation; D013329:Structure-Activity Relationship
D000903:Antibiotics, Antineoplastic; D005296:Ferrous Compounds; D001761:Bleomycin; C021771:zhengguangmycin