Cryogenic oven-trapping gas chromatography for analysis of volatile organic compounds in body fluids.
Cryogenic oven-trapping (COT) with capillary GC has been successfully applied to analysis of chloroform, dichloromethane, trichloroethylene, diethyl ether, the components of solvent thinner (ethyl acetate, benzene, n-butanol, toluene, and others), xylene isomers, cyanide, ethanol, hexanes, general anesthetics, and styrene in human body fluids. This COT-GC technique was compared with headspace solid-phase microextraction (SPME) coupled with GC for some volatile organic compounds (VOC); for all compounds compared the sensitivity achieved using COT-GC was more than ten times higher than for headspace SPME-GC. The COT-GC method is recommended for widespread use in forensic and environmental toxicology, because it is simple, requires no special GC operations, and yet enables high sensitivity and high resolution.
Watanabe-Suzuki
K
K
Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan. kanako@hama-med.ac.jp
Ishii
A
A
Suzuki
O
O
D001826:Body Fluids / Q000737:chemistry*; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D005615:Freezing; D006801:Humans; D009930:Organic Chemicals / Q000032:analysis*
D009930:Organic Chemicals