Rapid detection of Escherichia coli O157:H7 with multiplex real-time PCR assays.
A SYBR Green LightCycler PCR assay using a single primer pair allowed simultaneous detection of stx1 and/or stx2 of Escherichia coli O157:H7. A distinct sequence of the Shiga-like toxin genes was amplified to yield products of 227 and/or 224 bp, respectively. The two products were distinguished by melting point curve analysis.
Jothikumar
Narayanan
N
Department of Food Science and Canadian Research Institute for Food Safety, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Griffiths
Mansel W
MW
D003199:Computer Systems; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*