title: Application of liquid chromatography-mass spectrometry to the quantification of bisphenol A in human semen.
abstract: The potential risks to human health and reproduction from the xenoestrogen bisphenol A (BPA) have not been well established. This is due in part to the absence of accurate analytical methods to quantify BPA in biological samples. In this study we establish an accurate, sensitive and selective analytical method for the quantification of BPA in human semen. To quantify BPA we compared the techniques of liquid chromatography-mass spectrometry (LC-MS) and enzyme-linked immunosorbent assay (ELISA). In addition we have taken steps to eliminate BPA contamination during sample extraction and preparation. Results show that the ELISA method gives an over-estimate of BPA concentration, which may be due, at least in part, to non-specific interactions with the BPA-antibodies. LC-MS gave much more accurate results and proved to be more sensitive with a detection limit of 0.5 ng ml(-1) compared to 2.0 ng ml(-1) by ELISA.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Inoue
authors.forename: Megumi
authors.initials: M
authors.lastname: Wada
authors.forename: Tae
authors.initials: T
authors.lastname: Higuchi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Oshio
authors.forename: Takashi
authors.initials: T
authors.lastname: Umeda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazawa
mesh_terms: D001559:Benzhydryl Compounds; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D012661:Semen / Q000737:chemistry*; D012680:Sensitivity and Specificity; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds