Development of a genetic selection for catalytic antibodies.
The design and evaluation of a new genetic selection system for evolving catalytic antibodies with aldolase activity are described. Through a series of model selections, we have identified selection conditions where expression of a catalytically active antibody confers a growth advantage to Escherichia coli. In addition, we provide evidence that the growth advantage is a direct result of catalytic activity.
Gildersleeve
Jeff
J
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Janes
Jeff
J
Ulrich
Helle
H
Yang
Priscilla
P
Barbas
Carlos
C
Schultz
Peter G
PG
D017151:Antibodies, Catalytic / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000254:growth & development; D005634:Fructose-Bisphosphate Aldolase / Q000276:immunology*; D012641:Selection, Genetic
D017151:Antibodies, Catalytic; D017931:DNA Primers; D005634:Fructose-Bisphosphate Aldolase