title: Mitochondrial DNA polymorphisms and fertility in beef cattle.
abstract: Four hundred and twenty-two beef cattle of two different breeds (purebred Hereford and composite multibreed) were characterized by polymerase chain reaction-restriction fragment length polymorphism, using the restriction enzymes ApaI, AvaII, HindIII, PstI, SpeI, SspI and TaqI in two regions (the D-loop and the ND-5 gene) of mitochondrial DNA. The association between molecular haplotypes and records on calving rate, defined as the mean number of live calves born per year over 4 years, were examined by analysis of variance. A significant association was found between calving rate and mitochondrial polymorphisms in both breeds. This may have implications for genetically improving cow fertility.
authors.affiliation: Division of Veterinary and Biomedical Sciences, Murdoch University, Perth, WA, Australia.
authors.lastname: Sutarno
authors.forename: J M
authors.initials: JM
authors.lastname: Cummins
authors.forename: J
authors.initials: J
authors.lastname: Greeff
authors.forename: A J
authors.initials: AJ
authors.lastname: Lymbery
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000502:physiology; D004272:DNA, Mitochondrial / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005260:Female; D005298:Fertility / Q000235:genetics*; D008460:Meat; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy
chemical_list: D004272:DNA, Mitochondrial; C061568:endodeoxyribonuclease SspI; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C038485:GGGCCC-specific type II deoxyribonucleases; C038308:GGWCC-specific type II deoxyribonucleases; C027046:TCGA-specific type II deoxyribonucleases