title: Bone cement extracts modulate the osteoprotegerin/osteoprotegerin-ligand expression in MG63 osteoblast-like cells.
abstract: The osteoprotegerin-ligand (OPG-L) has been identified as the essential factor required for osteoclastogenesis, and its effects are prevented by the osteoprotegerin (OPG). The OPG-L/OPG balance plays a crucial role in coordinating the sequence of osteoclast and osteoblast differentiation during the bone remodeling. The aim of the study was to investigate if polymethylmethacrylate-based cements are able to modulate the expression of OPG-L/OPG in MG63 cells, which are known to have high levels of OPG and inducible expression of OPG-L. Four radio-opaque cements. namely Sulfix-60, CMW1, CMW2 and CMW3, were polymerized for either 1 h or 7 d. MG63 were incubated for 24 h with culture medium only, cement extracts and 2 microg/ml of human recombinant IL-1beta as positive control. An RT-PCR was performed to detect the OPG and OPG-L expression, and the house-keeping gene, GAPDH, was used as a reference for the semi-quantitative analysis. An increase in the OPG-L band density was observed for all cements, and consequently, the OPG-L/OPG ratio also increased. The ability of bone cements to induce the expression of OPG-L could be a co-factor in the development of osteolysis at the bone-cement interface.
authors.affiliation: Laboratorio di Fisiopatologia degli Impianti Ortopedici, Istituti Ortopedici Rizzoli, Bologna, Italy. donatella.granchi@ior.it
authors.forename: Donatella
authors.initials: D
authors.lastname: Granchi
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Cenni
authors.forename: Lucia
authors.initials: L
authors.lastname: Savarino
authors.forename: Gabriela
authors.initials: G
authors.lastname: Ciapetti
authors.forename: Giulia
authors.initials: G
authors.lastname: Forbicini
authors.forename: Melania
authors.initials: M
authors.lastname: Vancini
authors.forename: Cecilia
authors.initials: C
authors.lastname: Maini
authors.forename: Nicola
authors.initials: N
authors.lastname: Baldini
authors.forename: Armando
authors.initials: A
authors.lastname: Giunti
mesh_terms: D001843:Bone Cements / Q000009:adverse effects*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism*; D010014:Osteolysis / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D053244:Osteoprotegerin; D019904:Polymethyl Methacrylate / Q000009:adverse effects; D011475:Prosthesis Failure; D053245:RANK Ligand; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053246:Receptor Activator of Nuclear Factor-kappa B; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor
chemical_list: D001843:Bone Cements; D002352:Carrier Proteins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D053246:Receptor Activator of Nuclear Factor-kappa B; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C505686:TNFRSF11A protein, human; C506030:TNFRSF11B protein, human; C505688:TNFSF11 protein, human; D019904:Polymethyl Methacrylate