title: Effect of gamma-ray irradiated poly(L-lactide) on the differentiation of mouse osteoblast-like MC3T3-E1 cells.
abstract: The purpose of this study was to clarify the effects of gamma-ray irradiated poly(L-lactide) (PLLA) on the proliferation and differentiation of mouse osteoblast-like MC3T3-E1 cells. The PLLA was y-irradiated at the dose of 10, 25 or 50 kGy, and the molecular weight of irradiated PLLA decreased with increasing irradiation dose. The proliferation and differentiation of MC3T3-E1 cells cultured on irradiated PLLA for 2 weeks were evaluated using micromass culture. The y-irradiation of PLLA did not affect the proliferation, but stimulated the differentiation of MC3T3-E1 cells cultured on irradiated PLLA. These results suggested that lower change in the molecular weight of PLLA was responsible for stimulation of the differentiation of MC3T3-E1 cells cultured on irradiated PLLA. Furthermore, the proliferation and calcification of MC3T3-E1 cells cultured in the medium containing low molecular weight PLLA for 2 weeks were evaluated. The low molecular weight PLLA also stimulated the calcification of MC3T3-E1 cells with no effect on the proliferation. The y-irradiation was suitable for PLLA on the differentiation of mouse osteoblasts.
authors.affiliation: Division of Medical Devices, National Institute of Health Sciences, Tokyo, Japan. isama@nihs.go.jp
authors.forename: K
authors.initials: K
authors.lastname: Isama
authors.forename: T
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D016475:3T3 Cells; D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D004247:DNA / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006909:Hydroxyproline / Q000737:chemistry; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry*; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C033616:poly(lactide); D004247:DNA; D006909:Hydroxyproline; D002118:Calcium