Effect of hydrocortisone on the activity of some lysosomal enzymes in mice.
OBJECTIVE
Changes in the activity of cathepsin D and L, alanine aminopeptidase, leucine aminopeptidase, N-acetyl-beta-glucosaminidase, lysosomal arylesterase and lysosomal lipase in the liver and kidney of unselected and selected mice, subjected to 7.5 mg/kg b.w. of hydrocortisone injection for 4 and 8 days.


METHODS
The homogenates of the liver and kidney were subjected to differentiated centrifuging and determination of studied enzymes.


RESULTS
Injection of hydrocortisone caused an increase in the activity of all investigated lysosomal enzymes in the liver and kidney of mice.


CONCLUSIONS
The reactions of selected mice were stronger in comparison with unselected ones. The highest increase in the activity investigated enzymes was observed after 8 days of hydrocortisone injection.
Witek
Bozena
B
Department of Genetics, Institute of Biology, Swietokrzyska Academy, Kielce, Poland. b.witek@pu.kielce.pl
Ochwanowska
Ewa
E
Slewa
Alina
A
Kolataj
Adam
A
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D018826:CD13 Antigens / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism*; D056668:Cathepsin L; D002403:Cathepsins / Q000378:metabolism; D003546:Cysteine Endopeptidases; D004789:Enzyme Activation / Q000187:drug effects; D006706:Homeostasis / Q000187:drug effects; D006854:Hydrocortisone / Q000494:pharmacology*; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D051379:Mice
D000893:Anti-Inflammatory Agents; D008049:Lipase; D000118:Acetylglucosaminidase; D002403:Cathepsins; D007931:Leucyl Aminopeptidase; D018826:CD13 Antigens; D003546:Cysteine Endopeptidases; D056668:Cathepsin L; C534278:Ctsl protein, mouse; D002402:Cathepsin D; D006854:Hydrocortisone