An EVH1/WH1 domain as a key actor in TGFbeta signalling.
EVH1 (enabled VASP (vasodilator-stimulated protein) homology 1)/WH1 (WASP (Wiskott-Aldrich syndrome protein) homology 1) domains, present in Ena VASP and WASP, are protein interaction modules specialised in binding proline-rich ligands. An EVH1/WH1 domain is here identified in the recently cloned SMIF protein, a key protein in transforming growth factor-beta (TGFbeta) signalling which was not yet related to defined domains. The SMIF EVH1/WH1 domain interacts with the proline-rich Smad4 activation domain, leading to translocation of so-formed complex to the nucleus where SMIF possesses strong intrinsic TGFbeta-inducible transcriptional activity. This finding highlights the pivotal role that the EVH1/WH1 family of domains play in multiple eukaryotic signal transduction pathways.
Callebaut
Isabelle
I
Systèmes Moléculaires and Biologie Structurale, LMCP, CNRS UMR 7590, Universités Paris 6 and Paris 7, Case 115, 4 place Jussieu, Paris, France. isabelle.callebaut@lmcp.jussieu.fr
D000818:Animals; D001685:Biological Factors / Q000235:genetics / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000235:genetics; D016000:Cluster Analysis; D019295:Computational Biology; D017124:Conserved Sequence; D004331:Drosophila melanogaster; D006801:Humans; D008024:Ligands; D051379:Mice; D008840:Microfilament Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics; D011485:Protein Binding; D017434:Protein Structure, Tertiary / Q000502:physiology; D011506:Proteins / Q000235:genetics; D051381:Rats; D012441:Saccharomyces cerevisiae; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000502:physiology*; D051316:Wiskott-Aldrich Syndrome Protein; D000098662:Vasodilator-Stimulated Phosphoprotein
D001685:Biological Factors; D015815:Cell Adhesion Molecules; D008024:Ligands; D008840:Microfilament Proteins; D010750:Phosphoproteins; D011506:Proteins; D016212:Transforming Growth Factor beta; C495767:WAS protein, human; C495768:Was protein, mouse; C495769:Was protein, rat; D051316:Wiskott-Aldrich Syndrome Protein; D000098662:Vasodilator-Stimulated Phosphoprotein; C033031:smooth muscle inhibitory factor