title: High-performance liquid chromatographic analysis and separation of N-feruloylserotonin isomers.
abstract: The N-feruloylserotonin containing fraction was isolated from seeds of Leuzea carthamoides (Willd.) DC by solvent extraction followed by column chromatography on silica gel or on Sephadex LH-20. Nuclear magnetic resonance spectroscopic analysis of the isolated fraction showed the presence of four structurally related compounds. These compounds were identified as four isomers of N-feruloylserotonin: N-(Z)-feruloylserotonin, N-(Z)-isoferuloylserotonin, N-(E)-feruloylserotonin and N-(E)-isoferuloylserotonin. They were analyzed by HPLC on Separon SGX C18, Separon SGX and Separon SGX phenyl, using various mobile phases. Separon SGX phenyl phase was found the most efficient for a rapid analysis and for the final separation of the N-feruloylserotonin isomers.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague.
authors.forename: Milan
authors.initials: M
authors.lastname: Pavlík
authors.forename: Vera
authors.initials: V
authors.lastname: Laudová
authors.forename: Karel
authors.initials: K
authors.lastname: Grüner
authors.forename: Karel
authors.initials: K
authors.lastname: Vokác
authors.forename: Juraj
authors.initials: J
authors.lastname: Harmatha
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007536:Isomerism; D012701:Serotonin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012701:Serotonin