Cardiomyocyte differentiation of mouse and human embryonic stem cells.
Ischaemic heart disease is the leading cause of morbidity and mortality in the western world. Cardiac ischaemia caused by oxygen deprivation and subsequent oxygen reperfusion initiates irreversible cell damage, eventually leading to widespread cell death and loss of function. Strategies to regenerate damaged cardiac tissue by cardiomyocyte transplantation may prevent or limit post-infarction cardiac failure. We are searching for methods for inducing pluripotent stem cells to differentiate into transplantable cardiomyocytes. We have already shown that an endoderm-like cell line induced the differentiation of embryonal carcinoma cells into immature cardiomyocytes. Preliminary results show that human and mouse embryonic stem cells respond in a similar manner. This study presents initial characterization of these cardiomyocytes and the mouse myocardial infarction model in which we will test their ability to restore cardiac function.
Mummery
C
C
Hubrecht Laboratory, Utrecht, The Netherland. christin@niob.knaw.nl
Ward
D
D
van den Brink
C E
CE
Bird
S D
SD
Doevendans
P A
PA
Opthof
T
T
Brutel de la Riviere
A
A
Tertoolen
L
L
van der Heyden
M
M
Pera
M
M
D000328:Adult; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D017690:Cell Transplantation; D018920:Coculture Techniques; D004195:Disease Models, Animal; D004594:Electrophysiology; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D007473:Ion Channels / Q000378:metabolism; D051379:Mice; D009203:Myocardial Infarction / Q000503:physiopathology / Q000628:therapy*; D009206:Myocardium / Q000166:cytology*; D018408:Patch-Clamp Techniques; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D016277:Ventricular Function, Left
D007473:Ion Channels