[Induction of Th1 immune response against tumor by genetically engineered fusion of tumor cells and dendritic cells].
OBJECTIVE
To study the antitumor activity of engineered fusion of tumor cell and dendritic cells (DC).


METHODS
J558 tumor cells were transfected with mouse IL-12 (mIL-12) gene and then fused with DCs to develop a hybrid-engineered tumor vaccine. BALB/c mice were challenged with wild-type J558 tumor cells 14 days after vaccinated with hybrid-engineered J558.


RESULTS
mIL-12 was detected at (870 +/- 60) pg.(10(5) cells)(-1).ml(-1) in the culture supernatants and the cell-fusion rate was about 30% by co-focal microscopy. In addition, the lymphocytes from popliteal nodes and groin nodes of these mice vaccinated with hybrid-engineered J558 secreted higher levels of IFN-gamma than that of other control mice, and vaccination of mice with the fusion vaccine induced more efficient tumor-specific CTL cytotoxicity against wild-type tumor cells in vitro and with efficient antitumor immunity in vivo.


CONCLUSIONS
It suggested that vaccination of mice with the fusion vaccine induced stronger Th1-dominant responses and this approach could perhaps be applied to clinical settings of DCs-based cancer vaccines.
Zhang
Weidong
W
Department of Microbiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Yang
Hong
H
Zeng
Hongtao
H
Chen
Zhuang
Z
D000818:Animals; D002459:Cell Fusion; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D006822:Hybrid Cells / Q000166:cytology / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000378:metabolism / Q000401:mortality; D015996:Survival Rate; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000276:immunology
D018664:Interleukin-12; D007371:Interferon-gamma