Toxicogenomics; transcript profiling and potential application to chemical allergy.
Novel transcript profiling technologies allow simultaneous measurement of the changes in expression of many hundreds or many thousands of genes. The availability of these methods has brought about revolutionary changes in many areas of investigative biology, where analyses of patterns of gene expression, rather than of individual genes, are being employed. The application of these technologies to toxicology (toxicogenomics) offers new opportunities for both mechanistic toxicity research and predictive toxicology. Here we provide an overview of the basic approaches used in this field. The development of a series of toxicology-specific microarrays in our own laboratory is discussed, together with an example of one area of mechanistic research, chemical allergy, where we believe judicious application of toxicogenomics will make an important contribution.
Pennie
W D
WD
Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield SK10 4TJ, UK.
Kimber
I
I
D000818:Animals; D020869:Gene Expression Profiling; D023281:Genomics / Q000379:methods*; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D051381:Rats; D014116:Toxicology; D015262:Xenobiotics / Q000633:toxicity*
D015262:Xenobiotics