title: Embryonic lethality of mutant mice deficient in the p116 gene.
abstract: We report a lethal phenotype of mouse embryo with a disruption in the gene encoding p116, a subunit of the translation initiation factor, eIF3. The amino acid sequence of mouse p116, as deduced from the cDNA, shows high homology (97%) with human p116, and contains the conserved RNA binding sites, RNP1 and RNP2. The p116 mRNA is ubiquitously expressed in various organs, suggesting a house-keeping function of the p116 protein. To obtain genetic evidence for the essential role of the p116 protein in mouse cells, we constructed mice with a disruption in the p116 gene. Heterozygous p116(+/-) mice were intercrossed, and the genotypes of the offspring were determined. The results indicated no p116(-/-) pups among 84 neonates. Also, there were no p116(-/-) embryos 13.5 days postcoitum (d.p.c.). Among 77 embryos, there was only one p116(-/-) embryo at the blastocyst stage (3.5 d.p.c.). These results indicate that p116 plays an essential role in the early stages of mouse development.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
authors.forename: Rimiko
authors.initials: R
authors.lastname: Koyanagi-Katsuta
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Akimitsu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hamamoto
authors.forename: Nagisa
authors.initials: N
authors.lastname: Arimitsu
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Hatano
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Sekimizu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018387:Codon, Initiator; D018076:DNA, Complementary / Q000032:analysis; D004622:Embryo, Mammalian / Q000473:pathology*; D039621:Eukaryotic Initiation Factor-3 / Q000172:deficiency / Q000235:genetics*; D016678:Genome; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000172:deficiency / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D018387:Codon, Initiator; D018076:DNA, Complementary; D039621:Eukaryotic Initiation Factor-3; D021122:Protein Subunits