Ribonuclease H1 maps to chromosome 2 and has at least three pseudogene loci in the human genome.
We have analyzed the genomic structure of ribonuclease H1 (RNase H1) loci in the human genome. Human PAC library screening combined with database searches indicated that several loci are present. The transcribed gene is localized on chromosome 2p25. This was confirmed by RNA analysis of a monochromosomal hybrid cell line that expressed human chromosome 2. These data contradict a previous report, as well as the current Human Genome Project (HGP) annotation, which had placed the gene on chromosome 17p11.2. This location represents a pseudogene. Another highly similar pseudogene is present at a separate locus located more distal on chromosome 17p, while a third pseudogene is localized on chromosome 1q.
ten Asbroek
Anneloor L M A
AL
Neurozintuigen Laboratory, Academic Medical Center, 1000 DE Amsterdam, The Netherlands.
van Groenigen
Marjon
M
Jakobs
Marja E
ME
Koevoets
Cindy
C
Janssen
Bert
B
Baas
Frank
F
D001483:Base Sequence; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002886:Chromosomes, Human, Pair 17; D002889:Chromosomes, Human, Pair 2; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D011544:Pseudogenes; D016914:Ribonuclease H / Q000235:genetics*
D016914:Ribonuclease H; C078856:ribonuclease HI