Complete nucleotide sequence of plasmid Rts1: implications for evolution of large plasmid genomes.
Rts1, a large conjugative plasmid originally isolated from Proteus vulgaris, is a prototype for the IncT plasmids and exhibits pleiotropic thermosensitive phenotypes. Here we report the complete nucleotide sequence of Rts1. The genome is 217,182 bp in length and contains 300 potential open reading frames (ORFs). Among these, the products of 141 ORFs, including 9 previously identified genes, displayed significant sequence similarity to known proteins. The set of genes responsible for the conjugation function of Rts1 has been identified. A broad array of genes related to diverse processes of DNA metabolism were also identified. Of particular interest was the presence of tus-like genes that could be involved in replication termination. Inspection of the overall genome organization revealed that the Rts1 genome is composed of four large modules, providing an example of modular evolution of plasmid genomes.
Murata
Takahiro
T
Department of Bacteriology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
Ohnishi
Makoto
M
Ara
Takeshi
T
Kaneko
Jun
J
Han
Chang-Gyun
CG
Li
Yong Fang
YF
Takashima
Kayoko
K
Nojima
Hideaki
H
Nakayama
Keisuke
K
Kaji
Akira
A
Kamio
Yoshiyuki
Y
Miki
Takeyoshi
T
Mori
Hirotada
H
Ohtsubo
Eiichi
E
Terawaki
Yoshiro
Y
Hayashi
Tetsuya
T
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004261:DNA Replication; D004251:DNA Transposable Elements; D019143:Evolution, Molecular; D020131:Genes, Duplicate; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D011514:Proteus vulgaris / Q000235:genetics; D018741:Replication Origin / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004251:DNA Transposable Elements