title: Biosynthesis of cholestanol in higher plants.
abstract: To understand the early steps of C(27) brassinosteroid biosynthesis, metabolic experiments were performed with Arabidopsis thaliana and Nicotiana tabacum seedlings, and with cultured Catharanthus roseus cells. [26, 28-2H(6)]Campestanol, [26-2H(3)]cholesterol, and [26-2H(3)]cholestanol were administered to each plant, and the resulting metabolites were analyzed by gas chromatography-mass spectrometry. In all the species examined, [2H(3)]cholestanol was identified as a metabolite of [2H(6)]campestanol, and [2H(3)]cholest-4-en-3-one and [2H(3)]cholestanol were identified as metabolites of [2H(3)]cholesterol. This study revealed that cholestanol (C(27) sterol) was biosynthesized from both cholesterol (C(27) sterol) and campestanol (C(28) sterol). It was also demonstrated that cholestanol was converted to 6-oxocholestanol, and campestanol was converted to 6-oxocampestanol.
authors.affiliation: RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama 351-0198, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Nakajima
authors.forename: Shozo
authors.initials: S
authors.lastname: Fujioka
authors.forename: Takashi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Suguru
authors.initials: S
authors.lastname: Takatsuto
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D030008:Catharanthus / Q000378:metabolism; D004083:Cholestanol / Q000096:biosynthesis*; D002784:Cholesterol / Q000378:metabolism; D010840:Phytosterols / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism*
chemical_list: D010840:Phytosterols; C003535:campestanol; D004083:Cholestanol; D002784:Cholesterol