title: Basic fibroblast growth factor evokes a rapid glutamate release through activation of the MAPK pathway in cultured cortical neurons.
abstract: We examined the possibility that basic fibroblast growth factor (bFGF) is involved in synaptic transmissions. We found that bFGF rapidly induced the release of glutamate and an increase in the intracellular Ca2+ concentration through voltage-dependent Ca2+ channels in cultured cerebral cortical neurons. bFGF also evoked a significant influx of Na+. Tetanustoxin inhibited the bFGF-induced glutamate release, revealing that bFGF triggered exocytosis. The mitogen-activated protein kinase (MAPK) pathway was required for these acute effects of bFGF. We also found that pretreatment with bFGF significantly enhanced high K+-elicited glutamate release also in a MAPK activation-dependent manner. Therefore, we propose that bFGF exerts promoting effects on excitatory neuronal transmission via activation of the MAPK pathway.
authors.affiliation: Division of Protein Biosynthesis, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Numakawa
authors.forename: Daisaku
authors.initials: D
authors.lastname: Yokomaku
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Kiyosue
authors.forename: Naoki
authors.initials: N
authors.lastname: Adachi
authors.forename: Tomoya
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Numakawa
authors.forename: Takahisa
authors.initials: T
authors.lastname: Taguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hatanaka
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamada
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D005089:Exocytosis; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D020935:MAP Kinase Signaling System; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction; D012964:Sodium / Q000378:metabolism; D013744:Tetanus Toxin / Q000378:metabolism; D013997:Time Factors
chemical_list: D000596:Amino Acids; D013744:Tetanus Toxin; D016222:Fibroblast Growth Factor 2; D018698:Glutamic Acid; D005346:Fibroblast Growth Factors; D012964:Sodium; D011188:Potassium; D002118:Calcium