title: [Intracellular Ca2+ distribution and its role in mouse oocytes maturation].
abstract: To determine the role of calcium and calmodulin in mouse oocyte maturation, we examined the distribution of intracellular calcium during mouse oocyte maturation by using Mira Cal Imaging System. The calcium was present homogeneously in oocytes with intact germinal vesicle (GV) and accumulated around the nuclear region after GV breakdown(GVBD). The high level of calcium disappeared 6 hours later after GVBD. In the presence of 50 mumol/L BAPTA/AM, we failed to observe this phenomena. All eggs treated with 20 mumol/L W7, an antagonist of calmodulin, 50 mumol/L BAPTA/AM, a calcium chelator, could not develop to metaphase II (MII), although GVBD was not affected. We also detected the activity of a cytoplasmic maturation-promoting factor (MPF). W7 and BAPTA/AM had no effects on the rise of MPF activity in the course of maturation. We suggest that compartment distribution of calcium around nuclear region plays an important role in mouse oocyte maturation.
authors.affiliation: Laboratory of Molecular Developmental Biology, Institute of Developmental Biology, Chinese Academy of Sciences, Beijing 100080.
authors.forename: C G
authors.initials: CG
authors.lastname: Wang
authors.forename: M Q
authors.initials: MQ
authors.lastname: Deng
authors.forename: F Z
authors.initials: FZ
authors.lastname: Sun
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D002147:Calmodulin / Q000037:antagonists & inhibitors; D002614:Chelating Agents / Q000494:pharmacology; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D016200:Maturation-Promoting Factor / Q000378:metabolism; D000090204:Mesothelin; D051379:Mice; D009865:Oocytes / Q000254:growth & development*; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: D002147:Calmodulin; D002614:Chelating Agents; C000715528:Msln protein, mouse; D013449:Sulfonamides; D004533:Egtazic Acid; C017967:W 7; D016200:Maturation-Promoting Factor; D000090204:Mesothelin; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium