p53 and c-myc activation by Pasteurella haemolytica leukotoxin is correlated with bovine mononuclear cells apoptosis.
To analyse the role of Pasteurella haemolytica Leukotoxin (LKT) in the mechanism of apoptotic cell death of bovine lymphocytes, we evaluated DNA fragmentation and p53 and c-myc expression. P. haemolytica strain ATCC 14003 was cultivated for LKT production. DNA fragmentation was analysed by electrophoresis on Agarose gel. DNA strand breaks in individual apoptotic cells were also detected by an in situ Terminal deoxy nucleotidyl Transferase (TdT). The Polymerase Chain Reaction (PCR) procedure was used for verified p53 and c-myc activation by P. haemolytica LKT. LKT was able to induce DNA fragmentation in a dose and time-dependent fashion. The greatest apoptotic effect was obtained using LKT at a concentration of 0.25 U. The results show that p53 and c-myc activation by LKT is correlated with apoptosis of bovine lymphocytes and monocytes. Our data suggest that LKT may have an important role in the bacterial virulence of Pasteurella haemolytica.
Marcatili
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Dipartimento di Patologia Generale, Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, Italy.
D'Isanto
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M
Vitiello
M
M
Galdiero
R
R
Galdiero
M
M
D000818:Animals; D017209:Apoptosis; D001427:Bacterial Toxins / Q000378:metabolism*; D002417:Cattle; D053938:DNA Fragmentation; D004253:DNA Nucleotidylexotransferase / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005098:Exotoxins / Q000502:physiology*; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000473:pathology*; D016978:Mannheimia haemolytica / Q000737:chemistry*; D016271:Proto-Oncogene Proteins c-myc / Q000187:drug effects / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects / Q000378:metabolism*; D014774:Virulence
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; D016271:Proto-Oncogene Proteins c-myc; D016159:Tumor Suppressor Protein p53; C018579:leukotoxin; D004253:DNA Nucleotidylexotransferase