Preliminary Crystallographic Analysis of Phospholipase A(2) from Naja naja kaouthia Lesson Venom.
An acidic phospholipase A(2) isolated from the venom of Naja naja kaouthia Lesson in Guangxi exhibits anticoagulative and hemolytic activities. In this work, the enzyme was crystallized by the method of hanging drop vapor diffusion. Two crystal forms were obtained and characterized by X-ray diffraction. One of them belonged to space group P 4 ( 3 ) 2 ( 1 )2 or P4(1)2(1)2 with unit cell parameters a b 8.797 nm, c 10.831 nm and there were three molecules per asymmetric unit the other belonged to space group P2(1)3 with unit cell parameters a b c 6.840 nm and there was one molecule per asymmetric unit. The diffraction data were collected up to 0.28 nm for each crystal form. The crystal properties of Naja naja verom phospholipase A(2) from different geographical regions are compared.
Wang
Zhi-Qing
ZQ
National Laboratory of Biomacromolecules, Institute of Biophysics, the Chinese Academy of Sciences, Beijing 100101, China. lin@sun5.ibp.ac.cn
Zhuang
Mao-Xin
MX
Song
Shi-Ying
SY
Lin
Zheng-Jiong
ZJ
Shu
Yu-Yan
YY