Isolation and Analysis of hupR Gene Required for the Expression of Hydrogenase in Rhodobacter sphaeroides.
Cosmid 1 containing the hup genes isolated from the photosynthetic bacterium Rhodobacter sphaeroides was studied. The hupR gene from cosmid 1 was cloned and sequenced (EMBL accession number AJ243734). It encoded a 54.031 kD protein homologous to transcriptional regulators belonging to the superfamily of two-component regulatory systems. The HupR protein was overexpressed in Escherichia coli in the form of His6-tagged HupR. The cloned hupR gene could restore hydrogenase activity in R.sphaeroides hupR mutants and activate hupSL gene transcription.
Xu
D Q
DQ
Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Science, the Chinese Academy of Sciences, Shanghai 200032, China. yqwu@iris.sipp.ac.cn
Wu
Y Q
YQ