Isolation of genes encoding novel transcription factors which interact with the Hap complex from Aspergillus species.
The Saccharomyces cerevisiae CCAAT-binding factor is composed of four subunits Hap2p, Hap3p, Hap4p and Hap5p. Three subunits, Hap2/3/5p, are required for DNA-binding and Hap4p is involved in transcriptional activation. Although homologues of Hap2/3/5p (in the case of Aspergillus nidulans; HapB/C/E, respectively) were found in many eukaryotes, no Hap4p homologues have been found except for the other yeast, Kluyveromyces lactis. With the lexA-hap2, -hapB, -hapC, or -hapE fusion gene, we evaluated the ability of interaction between Aspergillus Hap subunits and S. cerevisiae Hap4p subunit in S. cerevisiae. Using the system with lexA-hapB, a gene encoding a novel transcriptional activator, which interacted with the Hap complex, was isolated from A. nidulans and designated hapX.
Tanaka
Akimitsu
A
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Kato
Masashi
M
Nagase
Takashi
T
Kobayashi
Tetsuo
T
Tsukagoshi
Norihiro
N
D000595:Amino Acid Sequence; D001230:Aspergillus / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D023081:CCAAT-Binding Factor / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D023081:CCAAT-Binding Factor; D005656:Fungal Proteins; C100785:HapC protein, Emericella nidulans; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C111072:aab-1 protein, Neurospora