High light-induced switch from C(3)-photosynthesis to Crassulacean acid metabolism is mediated by UV-A/blue light.
The high light-induced switch in Clusia minor from C(3)-photosynthesis to Crassulacean acid metabolism (CAM) is fast (within a few days) and reversible. Although this C(3)/CAM transition has been studied intensively, the nature of the photoreceptor at the beginning of the CAM-induction signal chain is still unknown. Using optical filters that only transmit selected wavelengths, the CAM light induction of single leaves was tested. As controls the opposite leaf of the same leaf pair was studied in which CAM was induced by high unfiltered radiation (c. 2100 micromol m(-2) s(-1)). To evaluate the C(3)-photosynthesis/CAM transition, nocturnal CO(2) uptake, daytime stomatal closure and organic acid levels were monitored. Light at wavelengths longer than 530 nm was not effective for the induction of the C(3)/CAM switch in C. minor. In this case CAM was present in the control leaf while the opposite leaf continued performing C(3)-photosynthesis, indicating that CAM induction triggered by high light conditions is wavelength-dependent and a leaf internal process. Leaves subjected to wavelengths in the range of 345-530 nm performed nocturnal CO(2) uptake, (partial) stomatal closure during the day (CAM-phase III), and decarboxylation of citric acid within the first 2 d after the switch to high light conditions. Based on these experiments and evidence from the literature, it is suggested that a UV-A/blue light receptor mediates the light-induced C(3)-photosynthesis/CAM switch in C. minor.
Grams
Thorsten E E
TE
GSF-National Research Center for Environment and Health, Institute of Soil Ecology, Department of Environmental Engineering, Ingolstädter Landstrasse, D-85764 Neuherberg, Germany.
Thiel
Stephan
S
D000222:Adaptation, Physiological / Q000502:physiology / Q000528:radiation effects*; D002245:Carbon Dioxide / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism; D028084:Clusiaceae / Q000145:classification / Q000502:physiology / Q000528:radiation effects; D029627:Crassulaceae / Q000145:classification / Q000378:metabolism* / Q000528:radiation effects; D003653:Decarboxylation / Q000528:radiation effects; D008293:Malates / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology* / Q000528:radiation effects; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism / Q000528:radiation effects*; D018515:Plant Leaves / Q000502:physiology / Q000528:radiation effects; D015398:Signal Transduction / Q000502:physiology / Q000528:radiation effects; D014466:Ultraviolet Rays; D014867:Water / Q000378:metabolism
D008293:Malates; D045322:Photosynthetic Reaction Center Complex Proteins; D014867:Water; D002245:Carbon Dioxide; D019343:Citric Acid; C030298:malic acid