title: Sequence analysis of the alpha trans-inducing factor of bovine herpesvirus type 5 (BHV-5).
abstract: Bovine herpesvirus (BHV), a member of the subfamily Alphaherpesvirinae, is classified into neurovirulent and non-neurovirulent subtypes on a basis of differential neuropathogenicities. Transcription of viral immediate early (IE) genes during alphaherpesvirus gene expression, is mediated by a multi-component immediate early complex (IEC) integrated by the viral tegument protein alpha trans-inducing factor (alpha-tif), a host cell protein (HCF), and a host Octamer protein (Oct). In this paper, we present a sequence analysis of the alpha-tif of the encephalitic BHV subtype, bovine herpesvirus type 5 (BHV-5). Bovine herpesvirus type 1 (BHV-1) and BHV-5 alpha-tifs share 98% amino acid sequence homology. However, BHV-5 alpha-tif is 23 residues shorter at the amino terminus than BHV-1 alpha-tif. Amino acid alignment of the alpha-tifs of BHV-1 and BHV-5 with other alphaherpesviruses indicates areas of conserved motifs but also important differences located mainly at the amino and carboxyl termini.
authors.affiliation: Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo 58105, USA. karin.gillette@ndsu.nodak.edu
authors.forename: Karin
authors.initials: K
authors.lastname: Gillette
authors.forename: Vikram
authors.initials: V
authors.lastname: Misra
authors.forename: Ana
authors.initials: A
authors.lastname: Bratanich
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D017405:Herpes Simplex Virus Protein Vmw65 / Q000737:chemistry / Q000235:genetics*; D025881:Herpesvirus 5, Bovine / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015533:Transcriptional Activation
chemical_list: D017405:Herpes Simplex Virus Protein Vmw65