The determination of protein phosphorylation on electrophoresis gel blots by laser ablation inductively coupled plasma-mass spectrometry.
Laser ablation interfaced with inductively coupled plasma-mass spectrometry is described as a new method to determine the presence of phosphorylated proteins on electrophoresis gel blots. The method was applied to the phosphoprotein beta-casein with good detection levels being observed at 16 pmole. Attempts at using the technique to detect beta-casein on electrophoresis gels are also described.
Marshall
Peter
P
Computational and Structural Sciences, GlaxoSmithKline, Stevenage, UK. PSM0667@gsk.com
Heudi
Olivier
O
Bains
Satty
S
Freeman
Hamzah Neil
HN
Abou-Shakra
Fadi
F
Reardon
Kevin
K
D002364:Caseins / Q000032:analysis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007834:Lasers; D010766:Phosphorylation
D002364:Caseins