title: Methamphetamine-induced apoptosis in a CNS-derived catecholaminergic cell line.
abstract: Methamphetamine (METH) causes neurotoxic damages to the dopaminergic system in mammals, but whether it exerts toxicity to dopamine cells in culture has not been fully explored. In order to develop an in vitro model of METH-induced dopamine neurotoxicity toward more systemical examination of the mechanism, we investigated METH toxicity in a clonal dopamine producing cell line (CATH.a). We show in the present study that METH produces a time- and dose-dependent increase in cell death via a process similar to apoptosis. The METH toxicity seems to be produced by oxidative stress, as it was attenuated by the antioxidant glutathione, and to involve dopamine because dopamine release and synthesis inhibitors attenuated the toxicity. This catecholaminergic cell line derived from the central nervous system may become a useful in vitro model to elucidate the mechanism underlying the METH-induced dopaminergic neuronal damage.
authors.affiliation: Department of Biochemistry, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Choi
authors.forename: Tae Moo
authors.initials: TM
authors.lastname: Yoo
authors.forename: Soo Youn
authors.initials: SY
authors.lastname: Chung
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Yang
authors.forename: Joo-Il
authors.initials: JI
authors.lastname: Kim
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Ha
authors.forename: Onyou
authors.initials: O
authors.lastname: Hwang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002490:Central Nervous System / Q000166:cytology / Q000502:physiology*; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D008694:Methamphetamine / Q000494:pharmacology* / Q000633:toxicity; D018384:Oxidative Stress; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D044005:Tetrahydroisoquinolines; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D007546:Isoquinolines; D011500:Protein Synthesis Inhibitors; D044005:Tetrahydroisoquinolines; D003609:Dactinomycin; D008694:Methamphetamine; C074266:4-phenyl-1,2,3,4-tetrahydroisoquinoline; D003513:Cycloheximide; D005978:Glutathione; D004298:Dopamine