title: Total parenteral nutrition-associated changes in mouse intestinal intraepithelial lymphocytes.
abstract: Intraepithelial lymphocytes (IEL) play a major role in mucosal defense mechanisms against intraluminal foreign antigens. To address the role luminal nutrients have on the phenotype and function of the IEL, we administered total parenteral nutrition (TPN) to mice, with the absence of enteral intake. We hypothesized that administration of TPN would result in changes in the phenotype and function of the IEL. For this, we utilized a mouse model of TPN. A significant decline in the CD4+ IEL population occurred with TPN. Additionally, the CD8+,CD44+ IEL subset showed a 65% decline (P < 0.05), and the CD4+,CD44+ subset declined by 55% with TPN (P < 0.05). The CD8alphabeta+ population (a marker of thymic-dependence) also declined by 92% (P < 0.01) with TPN. IEL in the TPN group showed a significantly lower degree of in vitro proliferation. In conclusion, the IEL showed significant phenotypic changes with TPN including the loss of the thymic-derived population. Functionally, the IEL showed a significant decline in proliferation. Such changes demonstrate the important role luminal nutrients have on IEL phenotype and function.
authors.affiliation: Department of Surgery, University of Michigan Medical School and C.S. Mott Children's Hospital, Ann Arbor 48109, USA.
authors.forename: Irfan
authors.initials: I
authors.lastname: Kiristioglu
authors.forename: Paul
authors.initials: P
authors.lastname: Antony
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Fan
authors.forename: Benjamin
authors.initials: B
authors.lastname: Forbush
authors.forename: R Lee
authors.initials: RL
authors.lastname: Mosley
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Teitelbaum
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D018960:Hyaluronan Receptors / Q000032:analysis; D007422:Intestines / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010289:Parenteral Nutrition, Total; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013047:Specific Pathogen-Free Organisms
chemical_list: D018960:Hyaluronan Receptors; D015375:Receptors, Interleukin-2