Global high-throughput screens for cellular function.
There is a critical need for global methods that allow for high-throughput assessment of cellular function for clinical and basic scientists working in both academia and the pharmaceutical industry. These methods typically couple systematic inactivation strategies with high-throughput cell-based assays that facilitate rapid target validation. We present here a survey of these technologies and their applications. We discuss their promise and limitations in addressing the vast number of candidate molecules of disease relevance that are emerging from genomics and proteomics.
Hauptschein
Robert S
RS
Department of Physiology, Tufts University School of Medicine, Boston, Mass., 02111, USA.
Eustace
Brenda K
BK
Jay
Daniel G
DG
D000818:Animals; D002468:Cell Physiological Phenomena; D005820:Genetic Testing; D006801:Humans; D008403:Mass Screening / Q000379:methods*