Influence of surface cell structures on physicochemical properties of Escherichia coli.
The partition of cells in a polyethylene glycol-dextran two phase system was used to compare the relative hydrophobicity of E. coli strains expressing different surface structures. The role of fimbriae and surface antigens on the behavior partition was investigated. The strains expressing PAP fimbriae and/or O-antigen showed a higher surface hydrophobicity than strains which express only type 1 fimbriae and/or R-antigen. No relation was found between K and H antigen and hydrophobicity measurements. The influence of surface structures on electrophoretic mobility has been evaluated. The polysaccharide capsules of AL 213 and AL 499 strains generated a high EPM. For non capsulated E. coli the EPM of rough strains (AL 46, 382) is higher than smooth strain (AL52).
El Ghmari
A
A
UFR de Chimie Appliquée et Sciences de l'Environnement, Laboratoire de Géosciences de l'Environnement, Faculté des Sciences et Techniques, Beni mellal, Maroc.
Latrache
H
H
Hamadi
F
F
El Louali
M
M
El Bouadili
A
A
Hakkou
A
A
Bourlioux
P
P
D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry*; D000954:Antigens, Surface / Q000737:chemistry*; D016667:Bacterial Capsules; D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D013499:Surface Properties
D000942:Antigens, Bacterial; D000954:Antigens, Surface