Sequence of a new DR12 allele with two silent mutations that affect PCR-SSP typing.
A new HLA-DR12 allele has been identified in a European Caucasoid bone marrow donor. The DRB1*12012 allele differs from DRB1*12011 by two silent substitutions at codons 72 and 78, two polymorphic positions used for DNA subtyping of the DR12 serotype. The co-occurence of the two nucleotide changes is unique to the DR12 group and results in a new PCR-SSP typing pattern. The complete HLA type of the donor is A24, A68; B55, B61; Cw*01, Cw*0304; DRB1*12012, DRB1*1402; DRB3*0101, DRB3*0202; DQB1*0301. HLA-DRB1*12012 is a rare allele as it occurs in < 0.2% of DR12 donors.
Zanone
R
R
National Reference Laboratory for Histocompatibility, Transplantation Immunology Unit, University Hospital, Geneva, Switzerland.
Bettens
F
F
Tiercy
J-M
JM
D000483:Alleles; D001483:Base Sequence; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D059810:HLA-DR Serological Subtypes; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D012689:Sequence Homology, Nucleic Acid
D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C082411:HLA-DR12 antigen