[DNA degradation in the leaves of the sugar beet (Beta vulgaris L.) in the process of senescence].
Programmed cell death (PCD) is a characteristic of all living beings in process of their development and in response to biotic and abiotic factors effects. On the pattern of the growing old 7-8th leaves of the sugar-beet (Beta vulgaris L.) of the first year vegetation of Belocerkovskaya singl-seed-45 sort character of the degradation and state of the methylation GTCGAC- and CTGCAG-sequences of the DNA were investigated. It was established by a method of electrophoresis that in the growing old leaves which didn't have any external indications of the senescence yet the DNA fragmentation didn't have place. The degradation of the total and nuclear DNA became intensive as far as senescence was gradually increasing with the age. The DNA degradation is accompanied by high level of its methylation in recognition sites of Pst I and Sal I which doesn't change during senscence of the leaves.
Topchiĭ
N M
NM
Taras Shevchenko Kyiv National University, Ukraine. topchiy_nataliya@univ.kiev.ua
D027461:Beta vulgaris / Q000235:genetics* / Q000502:physiology; D053938:DNA Fragmentation; D018744:DNA, Plant / Q000378:metabolism*; D006868:Hydrolysis; D018515:Plant Leaves / Q000235:genetics*
D018744:DNA, Plant