title: Comparison of nuclear morphometric parameters in cytologic smears and histologic sections of spontaneous canine tumors.
abstract: BACKGROUND
  Nuclear morphometry may provide useful diagnostic and prognostic information for neoplasms in animals. Most available data have been obtained from histologic sections. Nuclear morphometry of cytologic smears may provide important preoperative information.
  
  
  OBJECTIVE
  The goal of this study was to compare nuclear morphometric parameters in cytologic smears and histologic sections from spontaneous canine tumors.
  
  
  METHODS
  Mean nuclear area (MNA), mean nuclear perimeter (MNP), mean nuclear form factor (FF; nuclear perimeter(2)/4pi nuclear area) and their respective SDs were assessed by image analysis of both hematoxylin and eosin-stained histologic sections and May-Grünwald-Giemsa-stained cytologic smears from the same case in 20 spontaneous canine tumors of different histogenesis. The above parameters were selected as being the best morphometric tools for measuring variation in shape and size in cells after neoplastic transformation. Data were compared by ANOVA with P<.01 considered significant.
  
  
  RESULTS
  There was a significant difference between histologic and cytologic specimens for MNA, MNP, and their SDs. Only the differences between FF and the SD of FF were not statistically significant.
  
  
  CONCLUSIONS
  Only nuclear morphometric data related to nuclear shape and nuclear shape variability are comparable between histologic and cytologic specimens. Nuclear area and perimeter may be affected by the different fixation and smear preparation techniques used in histology and cytology.
authors.affiliation: Department of Veterinary Public Health, Unit of Pathology, Faculty of Veterinary Medicine, University of Messina, Messina, Italy. gdevico@unime.it
authors.forename: G
authors.initials: G
authors.lastname: De Vico
authors.forename: A
authors.initials: A
authors.lastname: Sfacteria
authors.forename: P
authors.initials: P
authors.lastname: Maiolino
authors.forename: G
authors.initials: G
authors.lastname: Mazzullo
mesh_terms: D000818:Animals; D003584:Cytological Techniques / Q000379:methods / Q000662:veterinary*; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000175:diagnosis / Q000473:pathology*; D004285:Dogs; D006652:Histological Techniques / Q000379:methods / Q000662:veterinary*; D019044:Image Cytometry / Q000379:methods / Q000662:veterinary; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D012189:Retrospective Studies; D013194:Staining and Labeling / Q000662:veterinary