title: Correct GPI-anchor synthesis is required for the incorporation of endoglucanase/glucanosyltransferase Bgl2p into the Saccharomyces cerevisiae cell wall.
abstract: The SSU21/MCD4 gene encodes an essential component of the glycosylphosphatidylinositol (GPI)-anchor synthesis pathway in Saccharomyces cerevisiae. Here we demonstrate that the ssu21 mutation affected the transport and the incorporation into the cell wall of the major non-GPI yeast cross-linker - endoglucanase/glucanosyltransferase Bgl2p. This mutation also led to a decrease in the levels of both known types of cell wall mannoproteins, those covalently linked with glucan and SDS-extractable proteins. Our results indicate that the precision of the GPI-anchor synthesis is essential for cell wall assembly and suggest the strong interdependence of different groups of cell wall proteins during their incorporation into the cell wall.
authors.affiliation: Molecular Biology Department, Faculty of Biology, Lomonosov Moscow State University, Vorobiovy Gory, 119899, Russia. kalebina@protein.bio.msu.ru
authors.forename: Tatyana S
authors.initials: TS
authors.lastname: Kalebina
authors.forename: Daniela K
authors.initials: DK
authors.lastname: Laurinavichiute
authors.forename: Anna N
authors.initials: AN
authors.lastname: Packeiser
authors.forename: Oleg S
authors.initials: OS
authors.lastname: Morenkov
authors.forename: Michael D
authors.initials: MD
authors.lastname: Ter-Avanesyan
authors.forename: Igor S
authors.initials: IS
authors.lastname: Kulaev
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols / Q000096:biosynthesis*; D016296:Mutagenesis; D011523:Protoplasts / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D017261:Glycosylphosphatidylinositols; D029701:Saccharomyces cerevisiae Proteins; C080389:BGL2 protein, S cerevisiae; D004693:Glucan Endo-1,3-beta-D-Glucosidase