Effect of presence or absence of corpora lutea on milk production in East Friesian dairy ewes.
The potential luteal effects on milk production were examined in dairy ewes that were not superovulated in contrast to studies using superovulated ewes. Lactating East Friesian crossbred ewes (n = 24) were synchronized using intravaginal progesterone (controlled intravaginal drug-releasing device), PGF2alpha, and gonadotropins. After ovulation, corpora lutea (CL) were counted via laparoscopy on d 4 and 11. On d 5, ewes received either saline (CLYES, n = 12) or PGF2alpha (CLNO, n = 12) to allow CL persistence (2.4 +/- 0.3 CL on d 11) or regression (0 CL on d 11), respectively. Each ewe received two CIDR d 5 to 18 to maintain high concentrations of plasma progesterone (P4) and to suppress estradiol (E2). Each ewe received PGF2alpha on d 18. Data were collected during three periods (pretreatment: d 0 to 5; treatment: d 6 to 18; posttreatment: d 19 to 25). Milk yield and milking time were recorded daily, milk samples were obtained for analyses of fat and protein, and blood samples were collected for P4 and E2 immunoassay. During treatment, CLYES ewes had higher milk yield (1.56 vs. 1.44 +/- 0.01 kg/d), milk fat (92.2 vs. 81.1 +/- 1.3 g/d), and milk protein (83.7 vs. 77.5 +/- 0.8 g/d) compared with CLNO ewes, respectively. Differences were maintained posttreatment, despite luteolysis in CLYES ewes. Estradiol concentrations did not differ between treatments and were low after d 5. Milk production was increased in East Friesian ewes in the presence of an average of 2.4 corpora lutea, an effect independent of estradiol.
McKusick
B C
BC
Department of Animal Sciences, University of Wisconsin, Madison 53706, USA. brett.mckusick@orionpharma.com
Wiltbank
M C
MC
Sartori
R
R
Marnet
P G
PG
Thomas
D L
DL
D000818:Animals; D003338:Corpus Luteum / Q000502:physiology*; D015237:Dinoprost / Q000494:pharmacology; D004958:Estradiol / Q000097:blood; D004973:Estrus Synchronization; D005260:Female; D007774:Lactation / Q000502:physiology*; D008055:Lipids / Q000032:analysis; D008892:Milk / Q000737:chemistry / Q000378:metabolism*; D008894:Milk Proteins / Q000032:analysis; D011374:Progesterone / Q000097:blood; D012756:Sheep / Q000502:physiology*; D013997:Time Factors
D008055:Lipids; D008894:Milk Proteins; D011374:Progesterone; D004958:Estradiol; D015237:Dinoprost