title: Expression of Exogenous E-cadherin Regulates Anchorage-independent Growth in Human Lung Adenocarcinoma Cells.
abstract: To explore the suppressive effects of E-cadherin on aggregation and anchorage-independent growth of human lung adenocarcinoma cells, a mammalian expression vector containing full E-cadherin cDNA was constructed and transfected into a metastatic human lung adenocarcinoma cell line A549. RT-PCR and Western blot were used to analyse expression levels of E-cadherin. Cells aggregation and anchorage-independent growth of the tumor cells before and after gene transfection were assessed respectively. The results indicated that stable transfectants showed markedly increased levels of expressed E-cadherin compared with the corresponding sham transfectants. The transfections showed more intense cellular aggregation and anchorage-independent growth.
authors.affiliation: Department of Biochemistry, Medical Center of Fudan University, Shanghai 200032, China. zhshen@shmu.edu.cn
authors.forename: Chuan-Xin
authors.initials: CX
authors.lastname: Huang
authors.forename: She
authors.initials: S
authors.lastname: Chen
authors.forename: Miao
authors.initials: M
authors.lastname: Ying
authors.forename: Zong-Hou
authors.initials: ZH
authors.lastname: Shen