Water stress, CO2 and photoperiod influence hormone levels in wheat.
'Super Dwarf' wheat (Triticum aestivum L.) plants have been grown from seed to maturity in the Mir space station where they were periodically exposed, because of microgravity and other constraints, to water deficit, waterlogging, high CO2 levels, and low light intensities. The plants produced many tillers, but none of them produced viable seed. Studies have been initiated to determine why the plants responded in these ways. In the present study, effects of the listed stresses on abscisic acid (ABA), indole-3-acetic acid (IAA) and isopentenyl adenosine ([9R]iP) levels in roots and leaves of plants grown under otherwise near optimal conditions on earth were measured. Hormones were extracted, purified by HPLC, and quantified by noncompetitive indirect ELISA. In response to water deficit, ABA levels increased in roots and leaves, IAA levels decreased in roots and leaves, and [9R]iP levels increased in leaves but decreased in roots. In response to waterlogging, ABA, IAA and [9R]iP levels briefly increased in roots and leaves and then decreased. When portions of the root system were exposed to waterlogging and/or water deficit, ABA levels in leaves increased while [9R]iP and IAA levels decreased. These responses were correlated with the percentage of the root system stressed. At a low photosynthetic photon flux (100 micromoles m-2 s-1), plants grown in continuous light had higher leaf ABA levels than plants grown using an 18 or 21 h photoperiod.
Nan
Rubin
R
Plants, Soils, and Biometeorology Department, Utah State University, Logan, UT 84322-4820, USA.
Carman
John G
JG
Salisbury
Frank B
FB
D000040:Abscisic Acid / Q000378:metabolism; D002245:Carbon Dioxide / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000378:metabolism; D007541:Isopentenyladenosine / Q000378:metabolism; D008027:Light; D017440:Photoperiod; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D014908:Triticum / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D014867:Water / Q000494:pharmacology*
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D014867:Water; D002245:Carbon Dioxide; C030737:indoleacetic acid; D000040:Abscisic Acid; D007541:Isopentenyladenosine