Sequencing of argentinated peptides by means of matrix-assisted laser desorption/ionization tandem mass spectrometry.
Argentinated peptide ions are formed in abundance under matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) conditions in the presence of Ag+ ions. These argentinated peptide ions are fragmented facilely under MALDI-MS/MS conditions to yield [b(n) + OH + Ag]+, [b(n) - H + Ag]+ and [a(n) - H + Ag]+ ions that are indicative of the C-terminal sequence. These observations parallel those made earlier under electrospray MS conditions (Chu, I. K; Guo, X.; Lau, T.-C.; Siu, K W. M. Anal. Chem. 1999, 71, 2364-2372). A mixed protonated and argentinated tryptic peptide map was generated from 37 fmol of bovine serum albumin (BSA) using MALDI-MS. MALDI-MS/MS data from four argentinated peptides at a protein amount of 350 fmol unambiguously identified the protein as BSA. Sequence-tag analysis of two argentinated tryptic peptides was used to identify unambiguously myocyte enhancer factor 2A, which had been recombinantly expressed in a bacterial cell line.
Chu
Ivan K
IK
Department of Chemistry, and Centre for Research in Mass Spectrometry, York University, Ontario, Canada.
Cox
David M
DM
Guo
Xu
X
Kireeva
Inga
I
Lau
Tai Chu
TC
McDermott
John C
JC
Siu
K W Michael
KW
D000818:Animals; D002417:Cattle; D004268:DNA-Binding Proteins / Q000032:analysis; D064326:MEF2 Transcription Factors; D018006:Myogenic Regulatory Factors; D010446:Peptide Fragments / Q000032:analysis*; D011506:Proteins / Q000032:analysis; D012680:Sensitivity and Specificity; D020539:Sequence Analysis, Protein / Q000295:instrumentation / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000378:metabolism; D012834:Silver / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014157:Transcription Factors / Q000032:analysis; D014357:Trypsin / Q000378:metabolism
D004268:DNA-Binding Proteins; D064326:MEF2 Transcription Factors; D018006:Myogenic Regulatory Factors; D010446:Peptide Fragments; D011506:Proteins; D014157:Transcription Factors; D012710:Serum Albumin, Bovine; D012834:Silver; D014357:Trypsin