title: Phospholipid and protein analysis of pulmonary surfactant.
abstract: Quantitative analysis of the phospholipid composition of pulmonary surfactant preparations has been achieved by a modification of the phosphorus-31 nuclear magnetic resonance method of E London and G Feigenson (J. Lipid Res. 20, 408-412, 1979). Resolution of the protein components by a 2-dimensional isoelectric-focussing-SDS/polyacrylamide-gel-electrophoresis technique is reported for the first time.
authors.affiliation: The British Industrial Biological Research Association, Carshalton, Surrey, UK.
authors.forename: R C
authors.initials: RC
authors.lastname: Cottrell
authors.forename: I A
authors.initials: IA
authors.lastname: Herod
authors.forename: D
authors.initials: D
authors.lastname: Bayley
authors.forename: R
authors.initials: R
authors.lastname: Purchase
authors.forename: K
authors.initials: K
authors.lastname: Miller
mesh_terms: D000818:Animals; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000662:veterinary; D007525:Isoelectric Focusing / Q000379:methods / Q000662:veterinary; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010743:Phospholipids / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D011663:Pulmonary Surfactants / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D010743:Phospholipids; D011506:Proteins; D011663:Pulmonary Surfactants