title: A second locus and new alleles in the major histocompatibility complex class II (ELA-DQB) region in the horse.
abstract: More than two nucleotide sequences of the second exon of the ELA-DQB region retrieved from a single animal and two different sequences isolated from horses homozygous in the major histocompatibility complex (MHC) region by descent indicated the existence of at least two ELA-DQB loci at the genomic level. New alleles detected by polymerase chain reaction single strand conformation polymorphism (SSCP) and defined by nucleotide sequencing of the second exon of the DQB gene(s) were described. Based on the level of nucleotide sharing, at least two groups of alleles were shown to exist. The newly defined alleles belonged preferentially to one of the groups. However, their specific locus assignment was not possible from the data collected. At least one of these alleles was shown to be transcribed. No frame-shift mutations were identified among the new alleles, although one pseudoallele containing a stop codon was identified at the genomic DNA level.
authors.affiliation: Institute of Animal Breeding and Genetics, Faculty of Veterinary Medicine, Brno, Czech Republic. horin.dior@ics.muni.cz
authors.forename: P
authors.initials: P
authors.lastname: Horín
authors.forename: J
authors.initials: J
authors.lastname: Matiasovic
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D018397:Equidae / Q000235:genetics*; D016678:Genome; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
chemical_list: D000949:Histocompatibility Antigens Class II