Increase in oxidative stress in kidneys of diabetic Akita mice.
New biomarkers for oxidative damage, were used to identify whether hyperglycemia caused oxidative stress in diabetic Akita mice. At 13 weeks of age, the tissues of these mice were obtained, and the levels of N(epsilon)-(hexanonyl)lysine (HEL) and dityrosine (DT) were measured, these being related to lipid peroxide-derived protein covalent modification and protein cross-linking. The levels of HEL and DT in the kidneys of Akita mice were significantly increased compared with the control mice without any accumulation of thiobarbituric acid reactive substances and 4-hydroxy-2-nonenal-modified protein. Immunopositive staining was clearly observed in the kidneys of the Akita mice when using the anti-HEL antibody or anti-DT antibody. These results suggest that hyperglycemia in Akita mice induced oxidative stress and increased these markers in the kidneys.
Ueno
Yuki
Y
Nagoya University Graduate School of Bioagricultural Sciences, Japan.
Horio
Fumihiko
F
Uchida
Koji
K
Naito
Michitaka
M
Nomura
Hideki
H
Kato
Yoji
Y
Tsuda
Takanori
T
Toyokuni
Shinya
S
Osawa
Toshihiko
T
D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D007668:Kidney / Q000473:pathology / Q000503:physiopathology*; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D014443:Tyrosine / Q000378:metabolism
D014443:Tyrosine; D008239:Lysine