Growth of AM fungi on in vitro root organ culture of Sorghum vulgare and Saccharum officinarum.
Spores of Gl mosseae and Gig gigantea germinated on minimal medium produced extraradical mycelium. Gl. mosseae infected roots of S. officinarum in in vitro condition were inoculated in M medium with in vitro cultured roots of Sorghum vulgare (test roots). From the infected root of S. officinarum, the mycelium developed and it infected the test roots. The roots developed new mycelia and further the mycelia produced a few hyaline spores. In MS medium combined with soil extract, root exudate, thiamine HCl and inositol combination, spore germination and germ tube growth were higher when compared with other media.
Raman
N
N
Centre for Advanced Studies in Botany, University of Madras, Chennai, India. raman55@vsnl.com
Sahadevan
C
C
Srinivasan
V
V
D046508:Culture Techniques; D005658:Fungi / Q000254:growth & development*; D018525:Germination; D018517:Plant Roots / Q000382:microbiology; D006109:Poaceae / Q000382:microbiology* / Q000502:physiology