Identification of a novel prokaryotic HEAT-repeats-containing protein which interacts with a cyanobacterial IscA homolog.
IscA homologs are known to be involved in iron-sulfur cluster formation in various organisms. Recombinant proteins of two IscA homologs from the cyanobacterium Synechocystis PCC 6803, designated SLR1417 and SLR1565, were purified. The absorption spectrum of purified SLR1565 was typical for [2Fe-2S] cluster-containing proteins, whereas that of SLR1417 predominantly showed the presence of the iron ion alone. In the cyanobacterial cell extracts, only SLR1565 was found to form a complex with a novel prokaryotic HEAT-repeats-containing protein, SLR1098. Thus, the two cyanobacterial IscA protein homologs exist in distinct molecular states, suggesting different cellular roles for these proteins.
Morimoto
Kozo
K
Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Japan.
Nishio
Kazuaki
K
Nakai
Masato
M
D016948:Azotobacter vinelandii; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002850:Chromatography, Gel; D000458:Cyanobacteria; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011485:Protein Binding; D020449:Repetitive Sequences, Amino Acid; D017386:Sequence Homology, Amino Acid; D013057:Spectrum Analysis; D013347:Subcellular Fractions / Q000737:chemistry
D001426:Bacterial Proteins; D002352:Carrier Proteins; D007506:Iron-Sulfur Proteins; D046911:Macromolecular Substances