title: Thermal stability of peroxidase from the african oil palm tree Elaeis guineensis.
abstract: The thermal stability of peroxidase from leaves of the African oil palm tree Elaeis guineensis (AOPTP) at pH 3.0 was studied by differential scanning calorimetry (DSC), intrinsic fluorescence, CD and enzymatic assays. The spectral parameters as monitored by ellipticity changes in the far-UV CD spectrum of the enzyme as well as the increase in tryptophan intensity emission upon heating, together with changes in enzymatic activity with temperature were seen to be good complements to the highly sensitive but integral method of DSC. The data obtained in this investigation show that thermal denaturation of palm peroxidase is an irreversible process, under kinetic control, that can be satisfactorily described by the two-state kinetic scheme, N -->(k) D, where k is a first-order kinetic constant that changes with temperature, as given by the Arrhenius equation; N is the native state, and D is the denatured state. On the basis of this model, the parameters of the Arrhenius equation were calculated.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Salamanca, Salamanca, Spain.
authors.forename: Anabel
authors.initials: A
authors.lastname: Rodríguez
authors.forename: David G
authors.initials: DG
authors.lastname: Pina
authors.forename: Belén
authors.initials: B
authors.lastname: Yélamos
authors.forename: John J
authors.initials: JJ
authors.lastname: Castillo León
authors.forename: Galina G
authors.initials: GG
authors.lastname: Zhadan
authors.forename: Enrique
authors.initials: E
authors.lastname: Villar
authors.forename: Francisco
authors.initials: F
authors.lastname: Gavilanes
authors.forename: Manuel G
authors.initials: MG
authors.lastname: Roig
authors.forename: Ivan Yu
authors.initials: IY
authors.lastname: Sakharov
authors.forename: Valery L
authors.initials: VL
authors.lastname: Shnyrov
mesh_terms: D028023:Arecaceae / Q000201:enzymology*; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D004795:Enzyme Stability; D006358:Hot Temperature; D009195:Peroxidase / Q000737:chemistry*; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence
chemical_list: D009195:Peroxidase