Human septin-septin interaction: CDCrel-1 partners with KIAA0202.
Septins are evolutionary conserved cytoskeletal GTPases forming heteropolymer complexes involved in cytokinesis and other cellular processes. CDCrel-1 (cell division cycle related-1) is a recently cloned and characterized human septin which is highly expressed in non-dividing cells, such as neurons. Using a yeast two-hybrid system we demonstrate that CDCrel-1 partners with another uncharacterized human septin, KIAA0202. The interaction of CDCrel-1 and KIAA0202 was confirmed in the human leukemia cell line K-562 using pull-down assays with a KIAA0202-glutathione S-transferase fusion protein and by immunoprecipitation of the CDCrel-1-KIAA0202 complex with an anti-KIAA0202 antibody. Expression studies of the two human septins revealed a concomitant expression of both proteins in certain cells.
Bläser
Susanne
S
Children's Hospital, University of Freiburg, Mathildenstr. 1, Freiburg, Germany.
Jersch
Katrin
K
Hainmann
Ina
I
Wunderle
Daniela
D
Zgaga-Griesz
Andrea
A
Busse
Anja
A
Zieger
Barbara
B
D000906:Antibodies / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D018797:Cell Cycle Proteins; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D020014:K562 Cells; D009206:Myocardium / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D058112:Septins; D020798:Two-Hybrid System Techniques
D000906:Antibodies; D018797:Cell Cycle Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D020558:GTP Phosphohydrolases; C109366:SEPTIN5 protein, human; D058112:Septins