title: Genotoxic effect of ozone in human peripheral blood leukocytes.
abstract: The genotoxic effect of ozone was studied in human leukocytes in vitro, using the single cell gel electrophoresis (SCGE) assay. Cell treatment for 1 h at 37 degrees C with 0.9-5.3 mM O(3) resulted in a dose-dependent increase of DNA damage, comparable to that induced by 4-40 mM of H(2)O(2), used as a positive control. This effect of ozone was reversed by post-treatment incubation of the cells for 45-90 min at 37 degrees C, and prevented by pre-incubation of the cells with catalase (20 microg/ml). These results demonstrate that O(3) induces DNA-damage in primary human leukocytes. The damage is rapidly repaired, and probably mediated by the formation of H(2)O(2).
authors.affiliation: Centro de Investigaciones y Evaluaciones Biológicas, Instituto de Farmacia y Alimentos, Universidad de La Habana, Apdo. 14024, C.P. 11400, Havana, Cuba. silvia@cieb.sld.cu
authors.forename: Silvia
authors.initials: S
authors.lastname: Díaz-Llera
authors.forename: Yanela
authors.initials: Y
authors.lastname: González-Hernández
authors.forename: E A
authors.initials: EA
authors.lastname: Prieto-González
authors.forename: Angel
authors.initials: A
authors.lastname: Azoy
mesh_terms: D002374:Catalase / Q000627:therapeutic use*; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology; D010126:Ozone / Q000494:pharmacology*
chemical_list: D010126:Ozone; D002374:Catalase; D010100:Oxygen