[Evaluation of the sensitivity of the indirect immunofluorescence test at the 1:20 dilution in the diagnosis of Chagas disease].
Sera from people in the chronic stage of Chagas disease, whose infection had been parasitologically validated, were assayed by using the indirect immunofluorescence test to evaluated its performance at the 1:20 dilution. All tests were consistently positive at 1:20 and higher dilutions, even in the presence of concomitant infection with the human immunodeficiency virus (HIV). It is thus valid, into the light of this experiment, to take into account the remarkable sensitivity of such serological test at the above mentioned dilution.
Amato Neto
Vicente
V
Laboratório de Parasitologia, Instituto de Medicina Tropical de São Paulo, Brasil. amatnet@usp.br
De Marchi
Cláudia Regina
CR
Rossitto
Sandra Therezinha
ST
Nascimento
Maria Savoia
MS
D014355:Chagas Disease / Q000175:diagnosis*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D007201:Indicator Dilution Techniques; D012680:Sensitivity and Specificity; D012698:Serologic Tests