title: Synthesis of (R)-1,3-butanediol by enantioselective oxidation using whole recombinant Escherichia coli cells expressing (S)-specific secondary alcohol dehydrogenase.
abstract: The synthesis of (R)-1,3-butanediol (BDO) from its racemate was studied using whole cells of recombinant Escherichia coli expressing an (S)-specific secondary alcohol dehydrogenase (CpSADH) from Candida parapsilosis by enantioselective oxidation. Under the optimized conditions, the yield of (R)-1,3-BDO reached 72.6 g/l, with a molar recovery yield of 48.4% from a racemate of 15% and an optical purity of 95% ee.
authors.affiliation: Tsukuba Research Center, Daicel Chemical Industries, Ltd., Ibaraki, Japan. h-yamamoto@daicel.co.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Akinobu
authors.initials: A
authors.lastname: Matsuyama
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kobayashi
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D002072:Butylene Glycols / Q000138:chemical synthesis*; D004926:Escherichia coli / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D002072:Butylene Glycols; D011994:Recombinant Proteins; C028491:1,3-butylene glycol; D000426:Alcohol Dehydrogenase