Separation of bisbenzylisoquinoline alkaloids by micellar electrokinetic chromatography.
The micellar electrokinetic chromatographic (MEKC) separation of seven bisbenzylisoquinoline alkaloids has been developed. The effects of various separating factors were studied. Optimum separation was achieved using a buffer (pH 9.2) of 20 mM sodium borate and 20 mM sodium dihydrogen phosphate buffer containing 55 mM sodium cholate; the optimum voltage and injection time were 21 kV and 0.05 min, respectively. Highest peak efficiency was obtained when the analytes were dissolved in 10 mM sodium dodecyl sulphate as sample matrix for injection. The elution order of the bisbenzylisoquinoline alkaloids was related to their lipophilicity. The resolution, run time and detection limits of the MEKC method were compared with those of an HPLC method developed previously.
Kuo
Ching-Hua
CH
School of Pharmacy, National Taiwan University, Taipei, Taiwan.
Sun
Shao-Wen
SW
D000470:Alkaloids / Q000737:chemistry*; D044182:Benzylisoquinolines; D002021:Buffers; D020355:Cholates; D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007546:Isoquinolines / Q000737:chemistry*; D019770:Lauraceae / Q000737:chemistry; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D012995:Solubility; D013997:Time Factors
D000470:Alkaloids; D044182:Benzylisoquinolines; D002021:Buffers; D020355:Cholates; D007546:Isoquinolines