Photosynthetic electron transport regulates the stability of the transcript for the protochlorophyllide oxidoreductase gene in the liverwort, Marchantia paleacea var. diptera.
The transfer of Marchantia paleacea var. diptera cells to darkness caused a reversible repression in the accumulation of transcript for a gene, por, encoding the NADPH: protochlorophyllide oxidoreductase (EC 1.3.1.33). The photosynthetic inhibitor DCMU and DBMIB repressed the accumulation in light. In the presence of transcription inhibitor cordycepin, not only incubation in the dark but also addition of DCMU or DBMIB in light stimulated the degradation of the por transcript. These findings suggest that photosynthetic electron transport is involved in regulating the stability of the por transcript.
Eguchi
Saeko
S
Graduate School of Science and Technology, Kumamoto University, Kurokami, Kumamoto, 860-8555 Japan.
Takano
Hiroyoshi
H
Ono
Kanji
K
Takio
Susumu
S
D003624:Darkness; D003839:Deoxyadenosines / Q000494:pharmacology; D003991:Dibromothymoquinone / Q000494:pharmacology; D004237:Diuron / Q000494:pharmacology; D004579:Electron Transport / Q000502:physiology; D008027:Light; D010088:Oxidoreductases / Q000235:genetics*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010788:Photosynthesis / Q000502:physiology*; D010944:Plants / Q000201:enzymology*; D020871:RNA Stability / Q000187:drug effects / Q000528:radiation effects; D018749:RNA, Plant / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D014158:Transcription, Genetic / Q000187:drug effects / Q000528:radiation effects
D003839:Deoxyadenosines; D018749:RNA, Plant; D003991:Dibromothymoquinone; D004237:Diuron; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C023886:protochlorophyllide reductase; C058120:cordycepin