title: Narrowing and genomic annotation of the commonly deleted region of the 5q- syndrome.
abstract: The 5q- syndrome is the most distinct of the myelodysplastic syndromes, and the molecular basis for this disorder remains unknown. We describe the narrowing of the common deleted region (CDR) of the 5q- syndrome to the approximately 1.5-megabases interval at 5q32 flanked by D5S413 and the GLRA1 gene. The Ensembl gene prediction program has been used for the complete genomic annotation of the CDR. The CDR is gene rich and contains 24 known genes and 16 novel (predicted) genes. Of 40 genes in the CDR, 33 are expressed in CD34(+) cells and, therefore, represent candidate genes since they are expressed within the hematopoietic stem/progenitor cell compartment. A number of the genes assigned to the CDR represent good candidates for the 5q- syndrome, including MEGF1, G3BP, and several of the novel gene predictions. These data now afford a comprehensive mutational/expression analysis of all candidate genes assigned to the CDR.
authors.affiliation: Leukaemia Research Fund Molecular Haematology Unit, Nuffield Department of Clinical Laboratory Science, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom. jboultwo@enterprise.molbiol.ox.ac.uk
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Boultwood
authors.forename: Carrie
authors.initials: C
authors.lastname: Fidler
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Strickson
authors.forename: Fiona
authors.initials: F
authors.lastname: Watkins
authors.forename: Susana
authors.initials: S
authors.lastname: Gama
authors.forename: Lyndal
authors.initials: L
authors.lastname: Kearney
authors.forename: Sabrina
authors.initials: S
authors.lastname: Tosi
authors.forename: Arek
authors.initials: A
authors.lastname: Kasprzyk
authors.forename: Jan-Fang
authors.initials: JF
authors.lastname: Cheng
authors.forename: Rina J
authors.initials: RJ
authors.lastname: Jaju
authors.forename: James S
authors.initials: JS
authors.lastname: Wainscoat
mesh_terms: D018952:Antigens, CD34; D002874:Chromosome Mapping / Q000379:methods; D002895:Chromosomes, Human, Pair 5; D017353:Gene Deletion; D006801:Humans; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D013234:Stem Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D018952:Antigens, CD34