title: Evidence that DNase I hypersensitive site 5 of the human beta-globin locus control region functions as a chromosomal insulator in transgenic mice.
abstract: We have previously reported that DNase I hypersensitive site 5 (5'HS5) of the human beta-globin locus control region functions as a chromatin insulator in stable transfection assays. In this report we show that a 3.2 kb DNA fragment containing the entire 5'HS5 region can protect a position-sensitive (A)gamma-globin gene against position effects in transgenic mice. Bracketing is required for function of 5'HS5 as an insulator. The 5'HS5 insulator operates in adult as well as in embryonic murine erythroid cells. The insulator has no significant stimulatory effects of its own. These results indicate that 5'HS5 can function as a chromatin insulator in vivo.
authors.affiliation: Division of Medical Genetics, Box 357720, Department of Medicine, University of Washington Medical School, Seattle, WA 98195, USA.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Li
authors.forename: Miaohua
authors.initials: M
authors.lastname: Zhang
authors.forename: Hemei
authors.initials: H
authors.lastname: Han
authors.forename: Alex
authors.initials: A
authors.lastname: Rohde
authors.forename: George
authors.initials: G
authors.lastname: Stamatoyannopoulos
mesh_terms: D000375:Aging / Q000097:blood / Q000235:genetics; D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003587:Cytomegalovirus / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D003850:Deoxyribonuclease I / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005914:Globins / Q000235:genetics*; D006801:Humans; D019426:Integrases / Q000235:genetics; D019901:Locus Control Region / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019076:Transgenes / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D015017:Yolk Sac / Q000378:metabolism
chemical_list: D002843:Chromatin; D004274:DNA, Recombinant; D012333:RNA, Messenger; D014764:Viral Proteins; D005914:Globins; C045073:Cre recombinase; D019426:Integrases; D003850:Deoxyribonuclease I