title: Cyclosporin A and transforming growth factor beta modify the pattern of extracellular glycosaminoglycans without causing cytoskeletal changes in human gingival fibroblasts.
abstract: Cyclosporin A is a powerful immunosuppressive drug that has had a major impact on transplant therapy. It apparently links to different enzymatic pathways, and affects multiple enzymatic systems. Transforming growth factor beta induces the deposition of glycosaminoglycans, proteoglycans, and collagen fibers in the extracellular matrix. The aim of this study of normal and hypertrophic human gingival fibroblast cultures was to evaluate the cytoskeletal and extracellular changes in glycosaminoglycan secretion due to the presence of cyclosporin A and transforming growth factor beta. The results showed that there is an increase in total and individual classes of extracellular glycosaminoglycans in the presence of cyclosporin A and transforming growth factor beta, but the action of the latter was significantly greater. Immunohistochemical analysis of the cytoskeleton did not reveal any morphological differences between treated and control cells. Our data suggest that the biochemical changes in the extracellular matrix are caused more by cytokine, and that cyclosporin A does not induce any morphological changes in fibroblast cultures derived from hypertrophic and normal gingiva.
authors.affiliation: Anatomy Department of Human-LITA Segrate, State University of Milan, Italy.
authors.forename: Giordano
authors.initials: G
authors.lastname: Stabellini
authors.forename: Francesco
authors.initials: F
authors.lastname: Carinci
authors.forename: Pier
authors.initials: P
authors.lastname: Luigi Bedani
authors.forename: Carla
authors.initials: C
authors.lastname: Calastrini
authors.forename: Monica
authors.initials: M
authors.lastname: De Mattei
authors.forename: Luca
authors.initials: L
authors.lastname: Scapoli
authors.forename: Angelo
authors.initials: A
authors.lastname: Caruso
authors.forename: Mario
authors.initials: M
authors.lastname: Calvitti
authors.forename: Paola
authors.initials: P
authors.lastname: Locci
mesh_terms: D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D003599:Cytoskeleton / Q000378:metabolism*; D005110:Extracellular Space / Q000187:drug effects / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D006025:Glycosaminoglycans; D007166:Immunosuppressive Agents; D016212:Transforming Growth Factor beta; D016572:Cyclosporine; D005944:Glucosamine