[Isolation, purification and physico-chemical properties of immunoactive constituents from the fruit of Lycium barbarum L].
Three glycoconjugates, LbGp3, LbGp4 and LbGp5, were isolated from the fruit of Lycium barbarum L. Molecular weights of LbGp3, LbGp4 and LbGp5 were 9.25 x 10(4), 21.48 x 10(4) and 2.37 x 10(4), respectively. Carbohydrate contents of LbGp3, LbGP4 and LbGp5 were 93.6%, 85.6%, 8.6%, respectively. LbGp3 was composed of Ara and Gal in a molar ratio of 1:1. LbGp4 was composed of Ara, Gal, Rha and Glc in a molar ratio of 1.5:2.5:0.43:0.23. LbGp5 was composed of Rha, Ara, Xyl, Gal, Man and Glc in a molar ratio of 0.33:0.52:0.42:0.94:0.85:1. Elemental analysis of N contents: LbGp3 0.83%, LbGp4 1.72%, LbGp5 9.58%. The linkage between the glycan and protein may be of O-linkage in LbGp4.
Huang
L
L
Shanghai Institute of Organic Chemistry, Academia Sinica, Shanghai 200032.
Lin
Y
Y
Tian
G
G
Ji
G
G
D000818:Animals; D005638:Fruit / Q000737:chemistry; D006001:Glycoconjugates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D032305:Lycium / Q000737:chemistry*; D008213:Lymphocyte Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
D006001:Glycoconjugates; D007166:Immunosuppressive Agents; D011134:Polysaccharides