[Receptor protein tyrosine kinase in splenic lymphocytes. Features of isolation and purification properties].
By means of an immune affinity chromatography we have obtained some receptor tyrosine protein kinase preparations from the spleen lymphocytes membrane in control and in 12 hours after rat X-ray irradiation in the doses of 0.5 and 1 Gy. An inhibitory analysis and estimation of essential catalytic properties of this enzyme have been performed. As a result of the researches performed we have suggested that EGF-R tyrosine protein kinases are involved in radiation-induced response of immune competent spleen cells and mediate the effects of EGF signal transduction in these cells in 12 hours after irradiation in the dose of 1 Gy.
Kirpenko
T O
TO
Kucherenko
M Ie
MIe
Ostapchenko
L I
LI
D000818:Animals; D002384:Catalysis; D004307:Dose-Response Relationship, Radiation; D066246:ErbB Receptors / Q000302:isolation & purification / Q000378:metabolism*; D008214:Lymphocytes / Q000201:enzymology* / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013154:Spleen / Q000166:cytology / Q000201:enzymology* / Q000528:radiation effects
D066246:ErbB Receptors