title: The sctR of Salmonella enterica serova Typhimurium encoding a homologue of MerR protein is involved in the copper-responsive regulation of cuiD.
abstract: We have identified the cuiD gene in Salmonella enterica serova Typhimurium that codes for a putative multicopper oxidase. Expression of cuiD is induced by copper ion and its promoter/operator has sequence similarity to the promoters controlled by the transcriptional regulators of the MerR family. We also identified and isolated a gene from S. enterica serova Typhimurium that encodes a 138-amino acid residue protein, sctR, a new member of the MerR family of transcriptional regulators. Transposon-insertional disruption of sctR shows sensitivity to copper ion and no response of cuiD expression. Copper-responsive induction and copper tolerance were restored by providing sctR in trans, suggesting that SctR plays an important role in intracellular copper detoxification.
authors.affiliation: Department of Microbiology, HanNam University, 133 Ojung-Dong, Taeduk-Ku, Taejon 306-791, South Korea.
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Kim
authors.forename: Mi Hwa
authors.initials: MH
authors.lastname: Kim
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Joe
authors.forename: Sang Sun
authors.initials: SS
authors.lastname: Song
authors.forename: In Soo
authors.initials: IS
authors.lastname: Lee
authors.forename: Soon-Yong
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003300:Copper / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009876:Operon; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001616:beta-Galactosidase / Q000187:drug effects / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C051124:MerR protein, Bacteria; C460088:SctR protein, Salmonella typhimurium; D014157:Transcription Factors; D003300:Copper; C460089:CiuD protein, Salmonella typhimurium; D010088:Oxidoreductases; C024748:copper oxidase; D001616:beta-Galactosidase