title: Synthesis of a novel class of polyhydroxyalkanoates in Arabidopsis peroxisomes, and their use in monitoring short-chain-length intermediates of beta-oxidation.
abstract: The poly[(R)-3-hydroxyalkanoate] (PHA) synthase gene (phaC(Ac)) of Aeromonas caviae FA440 was modified by adding a peroxisome targeting signal encoding the last 10 amino acids at the carboxyl-terminal of spinach glycolate oxidase. The modified gene was introduced into Arabidopsis thaliana plants by Agrobacterium-mediated transformation. The transgenic Arabidopsis plant expressed the introduced gene and its protein, and it accumulated PHA in its tissues. Gas chromatography-mass spectrometry analysis demonstrated the accumulation of a novel type of PHA, poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate). This strongly suggests that short-chain-length (R)-3-hydroxyacyl-CoAs were generated from intermediates of peroxisomal beta-oxidation. It was revealed by using this transgenic plant that Tween-20 can activate peroxisomal beta-oxidation of short-chain-length fatty acids.
authors.affiliation: RIKEN Institute, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198 Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Arai
authors.forename: Hideo
authors.initials: H
authors.lastname: Nakashita
authors.forename: Yoshikatu
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shimizu
authors.forename: Michiko
authors.initials: M
authors.lastname: Yasuda
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Doi
authors.forename: Isamu
authors.initials: I
authors.lastname: Yamaguchi
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000333:Aeromonas / Q000201:enzymology / Q000235:genetics; D016960:Agrobacterium tumefaciens / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D010084:Oxidation-Reduction / Q000187:drug effects; D020675:Peroxisomes / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011108:Polymers / Q000378:metabolism*; D011136:Polysorbates / Q000494:pharmacology; D012639:Seeds / Q000235:genetics; D000094122:Polyhydroxybutyrates
chemical_list: D000217:Acyltransferases; D005227:Fatty Acids; D011108:Polymers; D011136:Polysorbates; C435024:poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C077225:poly(3-hydroxyalkanoic acid) synthase