Rapid typing of Bordetella pertussis pertussis toxin gene variants by LightCycler real-time PCR and fluorescence resonance energy transfer hybridization probe melting curve analysis.
A LightCycler real-time PCR hybridization probe assay was developed for rapid typing of gene variants of the Bordetella pertussis virulence factor pertussis toxin. The assay correctly identified the ptxS1 alleles of all strains tested, comprising 57 Finnish clinical isolates and 2 vaccine strains. The method is simple, reliable, and suitable for large-scale screening of B. pertussis strains.
Mäkinen
Johanna
J
National Public Health Institute, Department in Turku, Finland. johanna.makinen@utu.fi
Mertsola
Jussi
J
Viljanen
Matti K
MK
Arvilommi
Heikki
H
He
Qiushui
Q
D000595:Amino Acid Sequence; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D001886:Bordetella pertussis / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D005456:Fluorescent Dyes; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015341:Nucleic Acid Probes; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013997:Time Factors; D010566:Virulence Factors, Bordetella / Q000235:genetics*
D005456:Fluorescent Dyes; D015341:Nucleic Acid Probes; D010567:Pertussis Vaccine; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin