High variability between results of different in-house tests for cytomegalovirus (CMV) monitoring and a standardized quantitative plasma CMV PCR assay.
A total of 2,718 blood samples were analyzed in five virological laboratories for the presence of cytomegalovirus (CMV) by in-house tests and one standardized plasma PCR assay. Results from in-house tests showed remarkable variability. Detection of CMV pp65 antigen or DNA from cells was more sensitive than that by plasma CMV PCR assay.
Von Müller
Lutz
L
Abteilung Virologie, Universität Ulm, Ulm, Germany.
Hampl
Walter
W
Hinz
Joachim
J
Meisel
Helga
H
Reip
Angela
A
Engelmann
Elisabeth
E
Heilbronn
Regine
R
Gärtner
Barbara
B
Krämer
Oliver
O
Einsele
Hermann
H
Hebart
Holger
H
Ljubicic
Tatjana
T
Löffler
Jürgen
J
Mertens
Thomas
T
D003470:Culture Media; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology; D004279:DNA, Viral / Q000097:blood*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006785:Hospitals, University; D006801:Humans; D007755:Laboratories, Hospital; D015588:Observer Variation; D010750:Phosphoproteins / Q000097:blood*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D015203:Reproducibility of Results; D014763:Viral Matrix Proteins / Q000097:blood*; D014773:Virology
D003470:Culture Media; D004279:DNA, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa