Nitric oxide synthase histochemistry in insect nervous systems: Methanol/formalin fixation reveals the neuroarchitecture of formaldehyde-sensitive NADPH diaphorase in the cockroach Periplaneta americana.
Formaldehyde-insensitive NADPH diaphorase (NADPHd) activity is used widely as a histochemical marker for neuronal nitric oxide synthase (NOS). However, in several insects including the cockroach Periplaneta americana, NOS is apparently formaldehyde-sensitive; NADPHd fails to reveal neuron morphology and results in faint generalized staining. Here we have used a novel fixative, methanol/ formalin (MF), to reveal for the first time the neuroarchitecture of NADPHd in the cockroach, with intense selective staining occurring in neurons throughout the brain and thoracic ganglia. Immunocytochemical and histochemical analysis of cockroach and locust nervous systems indicated that neuronal NADPHd after MF fixation can be attributed to NOS. However, NADPHd in locust glial and perineurial cells was histochemically different from that in neurons and may thus be due to enzymes other than NOS. Histochemical implications of species-specific enzyme properties and of the transcriptional complexity of the NOS gene are discussed. The present findings suggest that MF fixation is a valuable new tool for the comparative analysis of the neuroarchitecture of NO signaling in insects. The Golgi-like definition of the staining enabled analysis of the NADPHd architecture in the cockroach and comparison with that in the locust. NADPHd in the tactile neuropils of the thoracic ganglia showed a similar organization in the two species. The olfactory glomeruli of the antennal lobes were in both species densely innervated by NADPHd-positive local interneurons that correlated in number with the number of glomeruli. Thus, the NADPHd architectures appear highly conserved in primary sensory neuropils. In the cockroach mushroom bodies, particularly dense staining in the gamma-layer of the lobes was apparently derived from Kenyon cells, whereas extrinsic arborizations were organized in domains across the lobes, an architecture that contrasts with the previously described tubular compartmentalization of locust mushroom bodies. These divergent architectures may result in different spatiotemporal dynamics of NO diffusion and suggest species differences in the role of NO in the mushroom bodies.
Ott
Swidbert R
SR
School of Biological Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, United Kingdom. S.R.Ott@qmul.ac.uk
Elphick
Maurice R
MR
D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology; D003058:Cockroaches / Q000166:cytology / Q000201:enzymology*; D005260:Female; D005557:Formaldehyde; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000201:enzymology; D006110:Grasshoppers / Q000166:cytology / Q000201:enzymology*; D006651:Histocytochemistry / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008297:Male; D000432:Methanol; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009420:Nervous System / Q000166:cytology / Q000201:enzymology*; D009457:Neuroglia / Q000166:cytology / Q000201:enzymology; D026602:Nitrergic Neurons / Q000166:cytology / Q000201:enzymology; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D016707:Tissue Fixation / Q000379:methods*
D005557:Formaldehyde; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase; D000432:Methanol