Genetic relatedness among dioecious Ficus carica L. cultivars by random amplified polymorphic DNA analysis, and evaluation of agronomic and morphological characters.
A collection of 64 fig (Ficus carica L.) accessions was characterized through the use of RAPD markers, and results were evaluated in conjunction with morphological and agronomical characters, in order to determine the genetic relatedness of genotypes with diverse geographic origin. The results indicate that fig cultivars have a rather narrow genetic base. Nevertheless, RAPD markers could detect enough polymorphism to differentiate even closely related genotypes (i.e., clones of the same cultivar) and a unique fingerprint for each of the genotypes studied was obtained. No wasteful duplications were found in the collection. Cluster analysis allowed the identification of groups in accordance with geographic origin, phenotypic data and pedigree. Taking into account the limited information concerning fig cultivar development, the results of this study, which provide information on the genetic relationships of genetically distinct material, dramatically increase the fundamental and practical value of the collection and represent an invaluable tool for fig germplasm management.
Papadopoulou
Kalliopi
K
National Agricultural Research Foundation, Institute of Kalamata, Greece. nagrefkal@kal.forthnet.gr
Ehaliotis
Constantinos
C
Tourna
Maria
M
Kastanis
Petros
P
Karydis
Ioannis
I
Zervakis
Georgios
G
D030681:Ficus / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D010641:Phenotype; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique