[Host-guest molecule interaction mechanism of hemostatics with liposomes and red blood cells studied with fluorescence polarimetric method].
The supermolecule compounds of adrenobazone, p-aminomethylbenzoic acid, vitamin K1, 6-amino caproic acid with liposomes and red blood cells were studied by fluorescence polarimetric method. The mechanisms of formation of the supermolecule compounds were examined by fluorescence probe of the link of 1,6-dipheny-1,3,5-hexatriene (DPH) with liposomes which were taken as a model of blood cells. The interaction mechanism of hemostatics with red blood cells was described according the quantitative relationship between polarization value (P) and the microviscosity [formula: see text]. The result showed that the acting force between hemostatics and liposomes or that between hemostatics and red blood cells were mainly supermolecular acting force. The acting force between vitamin K1 and cytomembrane is hydrophobic force and those between adrenobazone, p-aminomethylbenzoic acid, or 6-amino caproic acid and cytomembrane are hydrogen bond or electrostatic force. Under the same drug concentration, all of the four haemostatics can reduce the fluidity of the cell membrane, which benefits blood coagulation. The binding ways of hemostatics with red blood cells was also discussed.
Du
M
M
Department of Chemistry, Shandong Normal University, Jinan 250014.
Tang
B
B
Shen
H
H
Wang
S
S
Sun
T
T
Zhang
C
C
D010129:4-Aminobenzoic Acid / Q000494:pharmacology; D000323:Adrenochrome / Q000031:analogs & derivatives* / Q000494:pharmacology; D015119:Aminocaproic Acid / Q000494:pharmacology; D000818:Animals; D001809:Blood Viscosity / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D005454:Fluorescence Polarization; D006490:Hemostatics / Q000494:pharmacology*; D008081:Liposomes / Q000378:metabolism*; D008560:Membrane Fluidity / Q000187:drug effects; D011817:Rabbits; D010837:Vitamin K 1 / Q000494:pharmacology; D062366:para-Aminobenzoates
D006490:Hemostatics; D008081:Liposomes; D062366:para-Aminobenzoates; C015668:4-aminomethylbenzoic acid; D000323:Adrenochrome; C073338:carbazochrome; D010837:Vitamin K 1; D010129:4-Aminobenzoic Acid; D015119:Aminocaproic Acid