[Receptor-mediated endocytosis induced macrophage cytosolic acidification and exocytosis].
The effects of Con A, WGA, Zymosan A on macrophage cytosolic pH and outflow of lysosomal content through exocytosis were studied with SNAFL-calcein and FITC-Dextran on ACAS570. The results showed all three ligands could induce macrophage cytosolic acidification in about 10 min and kept at the same level hereafter; outflow of lysosomal fluorescent probe through exocytosis appeared in 15-20 min. In resting conditions, macrophage lysosomes mainly distributed in cell center; after stimulated for 15 min by three ligands, the number of lysosomes increased in membrane periphery, in 25-30 min lysosomes moved back toward cell center. We proposed that ligands induced lysosomal pH rises was a basic factor for outflow of lysosomal content through exocytosis, cytosolic acidification inhibited receptor-mediated endocytosis. Cytosolic acidification and outflow of lysosomal content through exocytosis were the results of cellular self-regulation and self-protection during receptor-mediated endocytosis.
Lei
G H
GH
Central Laboratory, Department of Pediatrics of Nanfang Hospital, First Military Medical University, Guangzhou 510515.
Zhang
W
W
Wu
J C
JC
Piao
Y J
YJ
Bao
Y Y
YY
Huang
H
H
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D003593:Cytoplasm / Q000737:chemistry; D003600:Cytosol / Q000502:physiology; D004705:Endocytosis / Q000187:drug effects; D005089:Exocytosis / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D017737:Macrophages, Peritoneal / Q000737:chemistry / Q000502:physiology*; D008297:Male; D051379:Mice; D014909:Wheat Germ Agglutinins / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D015054:Zymosan