title: Expression of membrane-associated mucins MUC1 and MUC4 in major human salivary glands.
abstract: Mucins are high molecular weight glycoproteins secreted by salivary glands and epithelial cells lining the digestive, respiratory, and reproductive tracts. These glycoproteins, encoded in at least 13 distinct human genes, can be subdivided into gel-forming and membrane-associated forms. The gel-forming mucin MUC5B is secreted by mucous acinar cells in major and minor salivary glands, but little is known about the expression pattern of membrane-associated mucins. In this study, RT-PCR and Northern blotting demonstrated the presence of transcripts for MUC1 and MUC4 in both parotid and submandibular glands, and in situ hybridization localized these transcripts to epithelial cells lining striated and excretory ducts and in some serous acinar cells. The same cellular distribution was observed by immunohistochemistry. Soluble forms of both mucins were detected in parotid secretion after immunoprecipitation with mucin-specific antibodies. These studies have shown that membrane-associated mucins are produced in both parotid and submandibular glands and that they are expressed in different cell types than gel-forming mucins. Although the function of these mucins in the oral cavity remains to be elucidated, it is possible that they both contribute to the epithelial protective mucin layer and act as receptors initiating one or more intracellular signal transduction pathways.
authors.affiliation: Department of Periodontology, Boston University Medical Center, Boston, Massachusetts 02118, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Jessica R
authors.initials: JR
authors.lastname: Lague
authors.forename: David P
authors.initials: DP
authors.lastname: Nunes
authors.forename: Paul
authors.initials: P
authors.lastname: Toselli
authors.forename: Frank G
authors.initials: FG
authors.lastname: Oppenheim
authors.forename: Rodrigo V
authors.initials: RV
authors.lastname: Soares
authors.forename: Robert F
authors.initials: RF
authors.lastname: Troxler
authors.forename: Gwynneth D
authors.initials: GD
authors.lastname: Offner
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000378:metabolism*; D018396:Mucin-1 / Q000235:genetics / Q000378:metabolism*; D055264:Mucin-4; D009077:Mucins / Q000235:genetics / Q000378:metabolism*; D010306:Parotid Gland / Q000378:metabolism*; D011233:Precipitin Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013361:Sublingual Gland / Q000378:metabolism*; D013363:Submandibular Gland / Q000378:metabolism*
chemical_list: C524658:MUC4 protein, human; D008565:Membrane Proteins; D018396:Mucin-1; D055264:Mucin-4; D009077:Mucins