Complementary DNA sequence of the novel HLA-B*3704 allele.
The novel HLA-B*3704 allele was identified by polymerase chain reaction with sequence specific oligoneucletides (PCR-SSO) in a Spanish Caucasoid individual whose T-lymphocytes showed an ambiguous HLA-B phenotype. The nucleotide sequence of B*3704 was determined after reverse transcription-polymerase chain reaction (RT-PCR) amplification and molecular cloning of its complete coding region. B*3704 differs from B*3701 by a single nucleotide replacement that induces the substitution of histidine for tyrosine 171. Residue 171 is located in the alpha-helix of the alpha-2 domain, lining the A pocket of the peptide-binding site. Therefore, the His171 substitution seen in HLA-B*3704 is likely to affect its antigen-presenting properties and is probably responsible for the differentiated serological phenotype of this allele in comparison with B*3701.
Estefanía
E
E
Servicio de Inmunología, H.U. Clínica Puerta de Hierro, Madrid, Spain.
Gómez-Lozano
N
N
de Pablo
R
R
Moreno
M E
ME
Vilches
Carlos
C
D000483:Alleles; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015235:HLA-B Antigens / Q000235:genetics* / Q000276:immunology; D059912:HLA-B37 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017433:Protein Structure, Secondary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D044465:White People / Q000235:genetics
D018076:DNA, Complementary; D015235:HLA-B Antigens; D059912:HLA-B37 Antigen