title: Structural basis for the accessory protein recruitment by the gamma-adaptin ear domain.
abstract: The adaptor proteins AP-1 and GGA regulate membrane traffic between the trans-Golgi network (TGN) and endosomes/lysosomes through ARF-regulated membrane association, recognition of sorting signals, and recruitment of clathrin and accessory proteins. The gamma 1-adaptin subunits of AP-1 and GGA possess homologous ear domains involved in the recruitment of accessory proteins, gamma-synergin and Rabaptin-5. The crystal structure of the human gamma 1-adaptin ear domain consists solely of an immunoglobulin-like fold, unlike the alpha-adaptin ear domain. Structure-based mutational analyses reveal a binding site for the accessory proteins that is composed of conserved basic residues, indicating that the recruitment mechanism in gamma 1-adaptin and GGA is distinct from that in alpha-adaptin.
authors.affiliation: Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Tsukuba, Ibaraki 305-0801, Japan.
authors.forename: Terukazu
authors.initials: T
authors.lastname: Nogi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Shiba
authors.forename: Masato
authors.initials: M
authors.lastname: Kawasaki
authors.forename: Tomoo
authors.initials: T
authors.lastname: Shiba
authors.forename: Naohiro
authors.initials: N
authors.lastname: Matsugaki
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Igarashi
authors.forename: Mamoru
authors.initials: M
authors.lastname: Suzuki
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Kato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takatsu
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Nakayama
authors.forename: Soichi
authors.initials: S
authors.lastname: Wakatsuki
mesh_terms: D033982:Adaptor Protein Complex gamma Subunits; D000595:Amino Acid Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D020798:Two-Hybrid System Techniques
chemical_list: D033982:Adaptor Protein Complex gamma Subunits; D008565:Membrane Proteins; D021122:Protein Subunits