title: Recovery from release of ureteral obstruction in the rat: relationship to nephrogenesis.
abstract: BACKGROUND
  Obstructive nephropathy is a major cause of renal insufficiency in infants and children. Despite release of unilateral ureteral obstruction (UUO) in the first five days of life in the rat, renal growth is impaired, while glomerular filtration rate (GFR) is preserved at one month, but decreases markedly by one year. To test the hypothesis that renal recovery from UUO depends on the stage of nephrogenesis at the time of relief of obstruction, renal recovery from relief of five days UUO following completion of nephrogenesis (days 14 to 19) was compared with UUO during nephrogenesis (days 1 to 5).
  
  
  METHODS
  Rats underwent UUO or sham operation at one day of age, with relief five days later. In additional groups of neonatal rats, the operation was at 14 days, with relief at 19 days. Three months later, blood pressure, GFR, urine flow, sodium and potassium excretion, and kidney weight were measured. In addition, the number of glomeruli, glomerular maturation, glomerular diameter, tubular atrophy, and interstitial fibrosis were determined in each kidney. The effects of five-day UUO on number of glomeruli was determined also in adult rats one month following relief of obstruction.
  
  
  RESULTS
  Three months following relief of UUO during days 14 to 19, renal growth was decreased by 50%, compared to a 30% reduction following relief of UUO during days 1 to 5 (P < 0.05). The number of glomeruli was reduced by approximately 50% regardless of the timing of UUO, but glomerular size was reduced only in rats with UUO from days 14 to 19. Blood pressure and tubular atrophy were increased, and GFR, urine flow, sodium and potassium excretion were decreased in the postobstructed kidney of both neonatal groups. In the adult rat, the five-day UUO did not result in a decrease in the number of glomeruli.
  
  
  CONCLUSIONS
  In the period immediately following nephrogenesis, the kidney is particularly susceptible to long-term injury from temporary UUO. This suggests that a delay in relief of significant ureteral obstruction should be avoided if diagnosed in the perinatal or neonatal period.
authors.affiliation: Department of Pediatrics, University of Virginia, Charlottesville, Virginia 22908, USA. RLC2m@Virginia.edu
authors.forename: Robert L
authors.initials: RL
authors.lastname: Chevalier
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Thornhill
authors.forename: Alice Y
authors.initials: AY
authors.lastname: Chang
authors.forename: François
authors.initials: F
authors.lastname: Cachat
authors.forename: Adam
authors.initials: A
authors.lastname: Lackey
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D001284:Atrophy; D001794:Blood Pressure; D004231:Diuresis; D004573:Electrolytes / Q000652:urine; D005919:Glomerular Filtration Rate; D007668:Kidney / Q000254:growth & development* / Q000473:pathology / Q000503:physiopathology*; D007678:Kidney Glomerulus / Q000473:pathology; D007684:Kidney Tubules / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D014517:Ureteral Obstruction / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*
chemical_list: D004573:Electrolytes