title: Isolation of streptonigrin and its novel derivative from Micromonospora as inducing agents of p53-dependent cell apoptosis.
abstract: Streptonigrin (1) and its novel natural derivative 7-(1-methyl-2-oxopropyl)streptonigrin (2) were isolated from an actinomycete strain, Micromonospora sp. IM 2670. The inductions for 1 and 2 are more potent in the human neuroblastoma SH-SY5Y cells that contain wild-type p53 than in SH-SY5Y-5.6 cells that overexpress a dominant negative mutant of p53, thus suggesting that they induce apoptosis through a p53-dependent pathway.
authors.affiliation: Medicinal and Combinatorial Chemistry Laboratory, Lead Discovery Group, Microbial Collection, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Singapore.
authors.forename: Haishan
authors.initials: H
authors.lastname: Wang
authors.forename: Su Ling
authors.initials: SL
authors.lastname: Yeo
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: He
authors.forename: Francesca
authors.initials: F
authors.lastname: Ronca
authors.forename: Anthony E
authors.initials: AE
authors.lastname: Ting
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Victor C
authors.initials: VC
authors.lastname: Yu
authors.forename: Mui Mui
authors.initials: MM
authors.lastname: Sim
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002467:Cell Nucleus / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D016158:Genes, p53 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D020128:Inhibitory Concentration 50; D008847:Micromonospora / Q000737:chemistry*; D008968:Molecular Conformation; D015394:Molecular Structure; D009447:Neuroblastoma / Q000473:pathology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D013308:Streptonigrin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: C461041:7-(1-methyl-2-oxopropyl)streptonigrin; D016159:Tumor Suppressor Protein p53; D013308:Streptonigrin