Photothermal monitoring of redox state of respiratory chain in single live cells.
A method for monitoring respiratory chain (RC) activity in single live cells is described. It is based on the registration of the photothermal (PT) response after a laser pulse of a live single cell. The dependence of the PT-response amplitude and shape upon redox state of RC components was studied with a PT microscope for two in vitro models: (1) solutions of the RC component cytochrome c and (2) mice hepatocytes. The parameters of the PT responses differed for oxidized and reduced forms of cytochrome c solutions and for inhibited RC and intact RC. The latter difference may be caused by alteration of the quantum yields of thermal (nonradiative) relaxation for light-absorbing molecules, i.e. RC components, as they undergo reduction during RC inhibition.
Lapotko
Dmitri
D
Luikov Heat and Mass Transfer Institute, Minsk, Belarus. ld@hmti.ac.by
Romanovskaya
Tat'yana
T
Gordiyko
Elena
E
D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism* / Q000528:radiation effects; D007834:Lasers; D051379:Mice; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000502:physiology* / Q000528:radiation effects
D003574:Cytochrome c Group