Genetic homogeneity among Listeria monocytogenes strains from infected patients and meat products from two geographic locations determined by phenotyping, ribotyping and PCR analysis of virulence genes.
Thirty Listeria monocytogenes isolates from human patients and foods originated from two different geographic locations without any epidemiological relations were analyzed for their genotypic and phenotypic virulence gene expressions and genetic relatedness. All strains contained virulence genes, inlA, inlB, actA, hlyA, plcA and plcB, with expected product size in PCR assay except for the actA gene. Some strains produced actA gene product of 268 and others 385 bp. Phenotypically, all were hemolytic but showed variable expressions of phospholipase activity. Ribotyping classified isolates into 12 different groups based on the similarity to DuPont Identification numbers (DID), which consisted primarily of clinical or food isolates or both. Cluster analysis also indicated possible existence of clones of L. monocytogenes that are found in food or human hosts or are evenly distributed between these two. Two isolates (F1 from food and CHL1250 from patient) had unique ribotype patterns that were not previously reported in the RiboPrinter database. This study indicates distribution of diverse L. monocytogenes strains in clinical and food environments. The isolates showed 92-99% genetic homogeneity, in spite of their origins from two different geographic locations and environments.
Jaradat
Z W
ZW
Department of Food Science, Purdue University, West Lafayette, IN 47907-1160, USA.
Schutze
G E
GE
Bhunia
A K
AK
D000818:Animals; D005516:Food Microbiology; D005838:Genotype; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics*; D008461:Meat Products; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D021521:Ribotyping; D014774:Virulence / Q000235:genetics*