The immune responses of common carp, Cyprinus carpio L., injected with carp interleukin-1beta gene.
The function of the common carp, Cyprinus carpio L., interleukin-1beta (IL-1beta) gene was studied by DNA injection. To investigate the immune responses to IL-1beta, a plasmid construct of cytomegalovirus (CMV)-driven carp IL-1beta was injected into the epaxial muscle of carp. IL-1beta protein expressed in serum on 1, 3, and 5 days after plasmid injection was quantified by ELISA and immunohistochemistry. IL1-beta gene injection increased proliferation of the lymphocytes by phytohemagglutinin (PHA). Macrophage functions, such as production of superoxide anion and phagocytosis, also were stimulated by IL-1beta gene injection. Moreover, an increase in resistance to Aeromonas hydrophila infection was recorded in IL-1beta-injected fish compared with control fish. Thus, the cloned homolog of IL-1beta from carp has all the functional similarities to the mammalian IL-1beta gene.
Kono
Tomoya
T
United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan.
Fujiki
Kazuhiro
K
Nakao
Miki
M
Yano
Tomoki
T
Endo
Makoto
M
Sakai
Masahiro
M
D016980:Aeromonas hydrophila / Q000302:isolation & purification; D000818:Animals; D002347:Carps / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology; D018014:Gene Transfer Techniques; D007267:Injections; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics* / Q000502:physiology*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D010587:Phagocytosis; D013481:Superoxides / Q000378:metabolism
D007375:Interleukin-1; D013481:Superoxides; D004247:DNA