[Determination of elastase 1 in plasma or serum by latex turbidometric immunoassay with automatic analyzer].
We developed a latex turbidometric immunoassay(LTIA) for elastase 1. The precisions were 1.0-2.8% intra-run and 1.2-2.9% inter-day. The dilution test showed good linearity between 80-4000 ng/dl. There was interference by bilirubin, hemoglobin, lipemia or rheumatoid factor. Serum and plasma elastase 1 measured by LTIA(y) showed significant correlations with those by RIA(x) (serum: y = 0.98x--18, r = 0.980, n = 64, plasma: y = 0.96x--19, r = 0.976, n = 61). It was confirmed that the values were elevated in patients with pancreatic disease compared to the normal level. These results demonstrate that the determination of elastase 1 in plasma or serum by this method is useful, simple and time-saving, when compared with that by RIA.
Ohde
Katsuya
K
Iatron Laboratories, Inc., Chiba 289-2247.
Sawai
Tokio
T
D001322:Autoanalysis / Q000295:instrumentation* / Q000379:methods; D015415:Biomarkers / Q000097:blood; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007840:Latex; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation* / Q000379:methods; D010182:Pancreatic Diseases / Q000175:diagnosis; D010196:Pancreatic Elastase / Q000097:blood*
D015415:Biomarkers; D007840:Latex; D010196:Pancreatic Elastase