title: Molecular and biochemical characterization of a distinct type of fructose-1,6-bisphosphatase from Pyrococcus furiosus.
abstract: The Pyrococcus furiosus fbpA gene was cloned and expressed in Escherichia coli, and the fructose-1,6-bisphosphatase produced was subsequently purified and characterized. The dimeric enzyme showed a preference for fructose-1,6-bisphosphate, with a K(m) of 0.32 mM and a V(max) of 12.2 U/mg. The P. furiosus fructose-1,6-bisphosphatase was strongly inhibited by Li(+) (50% inhibitory concentration, 1 mM). Based on the presence of conserved sequence motifs and the substrate specificity of the P. furiosus fructose-1,6-bisphosphatase, we propose that this enzyme belongs to a new family, class IV fructose-1,6-bisphosphatase.
authors.affiliation: Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands. corne.verhees@algemeen.micr.wau.nl
authors.forename: Corné H
authors.initials: CH
authors.lastname: Verhees
authors.forename: Jasper
authors.initials: J
authors.lastname: Akerboom
authors.forename: Emile
authors.initials: E
authors.lastname: Schiltz
authors.forename: Willem M
authors.initials: WM
authors.lastname: de Vos
authors.forename: John
authors.initials: J
authors.lastname: van der Oost
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D006597:Fructose-Bisphosphatase / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D020027:Pyrococcus furiosus / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D019843:Archaeal Proteins; D006597:Fructose-Bisphosphatase