title: Effect of amino acids on thaxtomin A biosynthesis by Streptomyces scabies.
abstract: The regulatory effect of amino acids on the production of thaxtomin A, a phytotoxin produced by Streptomyces scabies, was investigated. Tryptophan had an important inhibitory effect on the toxin biosynthesis in all five strains of S. scabies tested. Two other aromatic amino acids (tyrosine and phenylalanine) also inhibited thaxtomin A biosynthesis, while aliphatic amino acids did not cause an important decline in thaxtomin A production. Methylation of tryptophan prevented or reduced the inhibitory effect on thaxtomin A biosynthesis. In spite of the inhibitory action of tryptophan and phenylalanine on thaxtomin A production, incorporation of these radiolabeled molecules into thaxtomin A confirmed that they are metabolic precursors for the biosynthesis of the phytotoxin.
authors.affiliation: Centre d'étude et de valorisation de la diversité microbienne, Département de biologie, Université de Sherbrooke, QC, Canada.
authors.forename: Annie
authors.initials: A
authors.lastname: Lauzier
authors.forename: Claudia
authors.initials: C
authors.lastname: Goyer
authors.forename: Luc
authors.initials: L
authors.lastname: Ruest
authors.forename: Ryszard
authors.initials: R
authors.lastname: Brzezinski
authors.forename: Don L
authors.initials: DL
authors.lastname: Crawford
authors.forename: Carole
authors.initials: C
authors.lastname: Beaulieu
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis; D007211:Indoles / Q000378:metabolism*; D010649:Phenylalanine / Q000502:physiology; D010879:Piperazines / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D013302:Streptomyces / Q000378:metabolism*; D014364:Tryptophan / Q000502:physiology
chemical_list: D001427:Bacterial Toxins; D007211:Indoles; D010879:Piperazines; C406691:thaxtomine A; D010649:Phenylalanine; D014364:Tryptophan