title: A stereologic evaluation of glucagon-like peptide-1 (GLP-1) mucosal cells in the small intestine of the developing pig.
abstract: Studies that investigate possible developmental changes in gastrointestinal hormones in the pig are sparse and contradictory. Therefore, a quantitative morphological study using stereologic methods on paraffin sections of the different small intestinal regions (cranial and caudal duodenum, jejunum and ileum) of the developing pig (second half of the gestation, neonatal and weaning period) has been conducted. The sections were processed for GLP-1-immunohistochemistry. During the investigated time span, the volume of GLP-1-IR cells increased approximately 40-fold in both the jejunum and ileum, notwithstanding a decrease of their volume density. The ileal small intestinal segment contained the highest volume density of GLP-1-IR cells. In contrast, GLP-1-IR cells were only occasionally encountered in the duodenum. The development-related changes of the investigated parameters coincide with dietary changes and the regional differences are in accordance with functional reports that point to GLP-1 as a gastrointestinal hormone that ediates the 'ileal brake' and stimulates glucose-dependent pancreatic insulin secretion.
authors.affiliation: Laboratory of Veterinary Anatomy and Embryology, Faculty of Medical and Pharmaceutical Sciences, University of Antwerp, Slachthuislaan 68, 2060 Antwerp, Belgium. chris.vanginneken@ua.ac.be
authors.forename: C
authors.initials: C
authors.lastname: Van Ginneken
authors.forename: K
authors.initials: K
authors.lastname: Verlinden
authors.forename: F
authors.initials: F
authors.lastname: Van Meir
authors.forename: S
authors.initials: S
authors.lastname: Sys
authors.forename: A
authors.initials: A
authors.lastname: Weyns
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D048429:Cell Size / Q000502:physiology; D005314:Embryonic and Fetal Development; D005934:Glucagon / Q000378:metabolism*; D052216:Glucagon-Like Peptide 1; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D013552:Swine / Q000502:physiology*
chemical_list: D010446:Peptide Fragments; D011498:Protein Precursors; D052216:Glucagon-Like Peptide 1; D005934:Glucagon