title: The C-terminal domain of the largest subunit of RNA polymerase II is required for stationary phase entry and functionally interacts with the Ras/PKA signaling pathway.
abstract: The Saccharomyces cerevisiae Ras proteins control cell growth by regulating the activity of the cAMP-dependent protein kinase (PKA). In this study, a genetic approach was used to identify cellular processes that were regulated by Ras/PKA signaling activity. Interestingly, we found that mutations affecting the C-terminal domain (CTD), of Rpb1p, the largest subunit of RNA polymerase II, were very sensitive to changes in Ras signaling activity. The Rpb1p CTD is a highly conserved, repetitive structure that is a key site of control during the production of a mature mRNA molecule. We found that mutations compromising the CTD were synthetically lethal with alterations that led to elevated levels of Ras/PKA signaling. Altogether, the data suggested that Ras/PKA activity was negatively regulating a protein that functioned in concert with the CTD during RNA pol II transcription. Consistent with this prediction, we found that elevated levels of Ras signaling caused growth and transcription defects that were very similar to those observed in mutants encoding an Rpb1p with a truncated CTD. In all, these data suggested that S. cerevisiae growth control and RNA pol II transcription might be coupled by using the Ras pathway to regulate CTD function.
authors.affiliation: Department of Molecular Genetics and Program in Molecular, Cellular, and Developmental Biology, Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Susie C
authors.initials: SC
authors.lastname: Howard
authors.forename: Yelena V
authors.initials: YV
authors.lastname: Budovskaya
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Chang
authors.forename: Paul K
authors.initials: PK
authors.lastname: Herman
mesh_terms: D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D005816:Genetic Complementation Test; D008957:Models, Genetic; D009154:Mutation; D010641:Phenotype; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012319:RNA Polymerase II / Q000737:chemistry* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D014158:Transcription, Genetic; D018631:ras Proteins / Q000378:metabolism*
chemical_list: D017868:Cyclic AMP-Dependent Protein Kinases; D012319:RNA Polymerase II; D018631:ras Proteins