title: In situ hybridization and immunogold localization of vascular endothelial growth factor receptor-2 on the pericytes of the chick chorioallantoic membrane.
abstract: This paper describes the expression of VEGF and of VEGFR-2 in the vasculature of the chorioallantoic membrane (CAM) as revealed by in situ hybridization and immunoelectron microscopy. Results showed that VEGFR-2 is expressed in both the endothelial cells and the pericytes, while VEGF in the chorionic epithelial cells. VEGF may therefore be released to promote both angiogenesis, by initiating an angiogenic response by endothelial cells expressing VEGFR-2, and the recruitment of pericytes along the capillary wall, playing also a crucial role in maturation and stabilization of the CAM blood vessels.
authors.affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy
authors.forename: Domenico
authors.initials: D
authors.lastname: Ribatti
authors.forename: Amauri
authors.initials: A
authors.lastname: Cruz
authors.forename: Beatrice
authors.initials: B
authors.lastname: Nico
authors.forename: Judith
authors.initials: J
authors.lastname: Favier
authors.forename: Angelo
authors.initials: A
authors.lastname: Vacca
authors.forename: Patrizia
authors.initials: P
authors.lastname: Corsi
authors.forename: Luisa
authors.initials: L
authors.lastname: Roncali
authors.forename: Franco
authors.initials: F
authors.lastname: Dammacco
mesh_terms: D000818:Animals; D002642:Chick Embryo; D016228:Endothelial Growth Factors / Q000096:biosynthesis / Q000378:metabolism; D004727:Endothelium / Q000166:cytology; D007150:Immunohistochemistry / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000378:metabolism; D008222:Lymphokines / Q000096:biosynthesis / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D018919:Neovascularization, Physiologic; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000096:biosynthesis*; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D040301:Vascular Endothelial Growth Factor Receptor-2