Synthesis of (R)-1,3-butanediol by enantioselective oxidation using whole recombinant Escherichia coli cells expressing (S)-specific secondary alcohol dehydrogenase.
The synthesis of (R)-1,3-butanediol (BDO) from its racemate was studied using whole cells of recombinant Escherichia coli expressing an (S)-specific secondary alcohol dehydrogenase (CpSADH) from Candida parapsilosis by enantioselective oxidation. Under the optimized conditions, the yield of (R)-1,3-BDO reached 72.6 g/l, with a molar recovery yield of 48.4% from a racemate of 15% and an optical purity of 95% ee.
Yamamoto
Hiroaki
H
Tsukuba Research Center, Daicel Chemical Industries, Ltd., Ibaraki, Japan. h-yamamoto@daicel.co.jp
Matsuyama
Akinobu
A
Kobayashi
Yoshinori
Y
D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D002072:Butylene Glycols / Q000138:chemical synthesis*; D004926:Escherichia coli / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000378:metabolism; D013237:Stereoisomerism
D002072:Butylene Glycols; D011994:Recombinant Proteins; C028491:1,3-butylene glycol; D000426:Alcohol Dehydrogenase