Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus.
The tobacco N gene confers resistance to tobacco mosaic virus (TMV) and encodes a Toll-interleukin-1 receptor/nucleotide binding site/leucine-rich repeat (TIR-NBS-LRR) class protein. We have developed and used a tobacco rattle virus (TRV) based virus induced gene silencing (VIGS) system to investigate the role of tobacco candidate genes in the N-mediated signalling pathway. To accomplish this we generated transgenic Nicotiana benthamiana containing the tobacco N gene. The transgenic lines exhibit hypersensitive response (HR) to TMV and restrict virus spread to the inoculated site. This demonstrates that the tobacco N gene can confer resistance to TMV in heterologous N. benthamiana. We have used this line to study the role of tobacco Rar1-, EDS1-, and NPR1/NIM1- like genes in N-mediated resistance to TMV using a TRV based VIGS approach. Our VIGS analysis suggests that these genes are required for N function. EDS1-like gene requirement for the N function suggests that EDS1 could be a common component of bacterial, fungal and viral resistance signalling mediated by the TIR-NBS-LRR class of resistance proteins. Requirement of Rar1- like gene for N-mediated resistance to TMV and some powdery mildew resistance genes in barley provide the first example of converging points in the disease resistance signalling pathways mediated by TIR-NBS-LRR and CC-NBS-LRR proteins. The TRV based VIGS approach as described here to study N-mediated resistance signalling will be useful for the analysis of not only disease resistance signalling pathways but also of other signalling pathways in genetically intractable plant systems.
Liu
Yule
Y
Department of Molecular, Cellular and Developmental Biology, OML 451, Yale University, PO Box 208104, New Haven, CT 06520-8104, USA.
Schiff
Michael
M
Marathe
Rajendra
R
Dinesh-Kumar
S P
SP
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D020868:Gene Silencing; D049452:Green Fluorescent Proteins; D007113:Immunity, Innate / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins; D008027:Light; D008164:Luminescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics; D014026:Nicotiana / Q000235:genetics* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000254:growth & development*
D029681:Arabidopsis Proteins; D036022:Capsid Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C118837:EDS1 protein, Arabidopsis; D047908:Intracellular Signaling Peptides and Proteins; D008164:Luminescent Proteins; C089425:N protein, Nicotiana glutinosa; C105496:NPR1 protein, Arabidopsis; D010940:Plant Proteins; C499582:Rar1 protein, Hordeum vulgare; C085220:coat protein, Tobacco rattle virus; D049452:Green Fluorescent Proteins