An integrated comparative map of the porcine X chromosome.
The objectives of this study were to assign both microsatellite and gene-based markers on porcine chromosome X to two radiation hybrid (RH) panels and to develop a more extensive integrated map of SSC-X. Thirty-five microsatellite and 20 gene-based markers were assigned to T43RH, and 16 previously unreported microsatellite and 15 gene-based markers were added to IMpRH map. Of these, 30 microsatellite and 12 gene-based markers were common to both RH maps. Twenty-two gene-based markers were submitted to BLASTN analysis for identification of orthologues of genes on HSA-X. Single nucleotide polymorphisms (SNPs) were detected for 12 gene-based markers, and nine of these were placed on the genetic map. A total of 92 known loci are present on at least one porcine chromosome X map. Thirty-seven loci are present on all three maps; 31 loci are found on only one map. Location of 33 gene-based markers on the comprehensive map translates into an integrated comparative map that supports conservation of gene order between SSC-X and HSA-X. This integrated map will be valuable for selection of candidate genes for porcine quantitative trait loci (QTLs) that map to SSC-X.
McCoard
S A
SA
USDA-ARS US Meat Animal Research Center, Clay Center, NE 68933-0166, USA.
Fahrenkrug
S C
SC
Alexander
L J
LJ
Freking
B A
BA
Rohrer
G A
GA
Wise
T H
TH
Ford
J J
JJ
D000818:Animals; D005819:Genetic Markers; D016678:Genome; D006801:Humans; D021101:Radiation Hybrid Mapping; D013552:Swine / Q000235:genetics*; D014960:X Chromosome
D005819:Genetic Markers