Cloning and characterization of the deoxyribonuclease sd alpha gene from Streptococcus pyogenes.
The pathogenesis of Streptococcus pyogenes infection, especially toxic shock-like syndrome (TSLS), is still not fully understood; however, the exoproteins have been considered to play a role. We analyzed the culture supernatant proteins (exoproteins) from a TSLS-related isolate belonging to M3 serotype S. pyogenes by two-dimensional gel electrophoresis and characterized a single protein spot by using BLAST database. We cloned the gene of this protein and named it sd alpha, which was similar to the deoxyribonuclease (DNase) sdc of S. equisimilis. We showed that the recombinant protein from the sd alpha gene had DNase activity. By polymerase chain reaction, we found that the sd alpha gene was present in most clinically isolated S. pyogenes including TSLS-related isolates. We thus conclude that Sd alpha is a new DNase of S. pyogenes.
Hasegawa
Tadao
T
Department of Molecular Bacteriology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan. hasegawa@med.nagoya-u.ac.jp
Torii
Keizo
K
Hashikawa
Shinnosuke
S
Iinuma
Yoshitsugu
Y
Ohta
Michio
M
D003001:Cloning, Molecular; D003851:Deoxyribonucleases / Q000235:genetics* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis; D017422:Sequence Analysis, DNA; D012772:Shock, Septic / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity
D011994:Recombinant Proteins; D003851:Deoxyribonucleases