title: Thymidine phosphorylase is regulated by tamoxifen in T47D breast cancer cell line.
abstract: BACKGROUND
  Thymidine phosphorylase (TP) has diverse functions within cells, including increasing the sensitivity of cancer cells to cytotoxic drugs including 5-fluorouracil (5-FU) and methotrexate. However, the regulators of TP still remains largely unknown.
  
  
  METHODS
  In this study, we examined whether tamoxifen has specific effects on TP expression in the T47D breast cancer cell line. We studied the TP expression in the T47D cell line before and after tamoxifen treatment using Western blot analysis and ELISA.
  
  
  RESULTS
  We found that TP expression is significantly up-regulated in tamoxifen-treated cells compared with control.
  
  
  CONCLUSIONS
  Our findings suggest that tamoxifen may increase chemotherapy sensitivity through TP up-regulation for treatment regimens including doxifluridine, capecitabine, and/or methotrexate.
authors.affiliation: Hirotoshi Utsunomiya, Clinical and Basic Research Institute of Molecular Cellular Biology and Bioengineering, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-0012, Japan. utsu@wakayama-med.ac.jp
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Utsunomiya
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ueshima
authors.forename: Mari
authors.initials: M
authors.lastname: Kawashiro
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Yang
authors.forename: Misa
authors.initials: M
authors.lastname: Nakamura
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakurai
authors.forename: Ichiro
authors.initials: I
authors.lastname: Mori
authors.forename: Kennichi
authors.initials: K
authors.lastname: Kakudo
mesh_terms: D015153:Blotting, Western; D001943:Breast Neoplasms / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D011336:Probability; D012016:Reference Values; D012680:Sensitivity and Specificity; D013629:Tamoxifen / Q000494:pharmacology*; D013939:Thymidine Phosphorylase / Q000032:analysis* / Q000187:drug effects*; D014407:Tumor Cells, Cultured
chemical_list: D013629:Tamoxifen; D013939:Thymidine Phosphorylase