title: Complete nucleotide sequence of plasmid Rts1: implications for evolution of large plasmid genomes.
abstract: Rts1, a large conjugative plasmid originally isolated from Proteus vulgaris, is a prototype for the IncT plasmids and exhibits pleiotropic thermosensitive phenotypes. Here we report the complete nucleotide sequence of Rts1. The genome is 217,182 bp in length and contains 300 potential open reading frames (ORFs). Among these, the products of 141 ORFs, including 9 previously identified genes, displayed significant sequence similarity to known proteins. The set of genes responsible for the conjugation function of Rts1 has been identified. A broad array of genes related to diverse processes of DNA metabolism were also identified. Of particular interest was the presence of tus-like genes that could be involved in replication termination. Inspection of the overall genome organization revealed that the Rts1 genome is composed of four large modules, providing an example of modular evolution of plasmid genomes.
authors.affiliation: Department of Bacteriology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Murata
authors.forename: Makoto
authors.initials: M
authors.lastname: Ohnishi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ara
authors.forename: Jun
authors.initials: J
authors.lastname: Kaneko
authors.forename: Chang-Gyun
authors.initials: CG
authors.lastname: Han
authors.forename: Yong Fang
authors.initials: YF
authors.lastname: Li
authors.forename: Kayoko
authors.initials: K
authors.lastname: Takashima
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojima
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nakayama
authors.forename: Akira
authors.initials: A
authors.lastname: Kaji
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kamio
authors.forename: Takeyoshi
authors.initials: T
authors.lastname: Miki
authors.forename: Hirotada
authors.initials: H
authors.lastname: Mori
authors.forename: Eiichi
authors.initials: E
authors.lastname: Ohtsubo
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Terawaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hayashi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004261:DNA Replication; D004251:DNA Transposable Elements; D019143:Evolution, Molecular; D020131:Genes, Duplicate; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D011514:Proteus vulgaris / Q000235:genetics; D018741:Replication Origin / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements