title: Antisense and chemical suppression of the nonmevalonate pathway affects ent-kaurene biosynthesis in Arabidopsis.
abstract: Transgenic plants of Arabidopsis thaliana (L.) Heynh. (ecotype Columbia) expressing the antisense AtMECT gene, encoding 2- C-methyl- D-erythritol 4-phosphate cytidylyltransferase, were generated to elucidate the physiological role of the nonmevalonate pathway for production of ent-kaurene, the latter being the plastidic precursor of gibberellins. In transformed plants pigmentation and accumulation of ent-kaurene were reduced compared to wild-type plants. Fosmidomycin, an inhibitor of 1-deoxy- D-xylulose 5-phosphate reductoisomerase (DXR), caused a similar depletion of these compounds in transgenic plants. These observations suggest that both AtMECT and DXR are important in the synthesis of isopentenyl diphosphate and dimethylallyl diphosphate and that ent-kaurene is mainly produced through the nonmevalonate pathway in the plastid.
authors.affiliation: Department of Biology, Tokyo Gakugei University, Nukuikitamachi 4-1-1, Koganei-shi, Tokyo, 184-8501, Japan. kokada@u-gakugei.ac.jp
authors.forename: Kazunori
authors.initials: K
authors.lastname: Okada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawaide
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Kuzuyama
authors.forename: Haruo
authors.initials: H
authors.lastname: Seto
authors.forename: Ian S
authors.initials: IS
authors.lastname: Curtis
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kamiya
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000037:antagonists & inhibitors; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002338:Carotenoids / Q000096:biosynthesis; D002734:Chlorophyll / Q000096:biosynthesis; D000077194:Chlorophyll A; D002736:Chloroplasts / Q000378:metabolism; D003001:Cloning, Molecular; D016373:DNA, Antisense / Q000235:genetics; D004224:Diterpenes / Q000378:metabolism*; D045786:Diterpenes, Kaurane; D005578:Fosfomycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045782:Hemiterpenes; D008798:Mevalonic Acid / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors; D009713:Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010088:Oxidoreductases / Q000037:antagonists & inhibitors; D010641:Phenotype; D010860:Pigments, Biological / Q000096:biosynthesis; D030821:Plants, Genetically Modified
chemical_list: D016373:DNA, Antisense; D004224:Diterpenes; D045786:Diterpenes, Kaurane; D045782:Hemiterpenes; D009097:Multienzyme Complexes; D009943:Organophosphorus Compounds; D010860:Pigments, Biological; D002734:Chlorophyll; D005578:Fosfomycin; C002627:kaurene; C004809:isopentenyl pyrophosphate; D002338:Carotenoids; C037184:chlorophyll b; C024640:fosmidomycin; D010088:Oxidoreductases; C114231:1-deoxy-D-xylulose 5-phosphate reductoisomerase; C435808:2-C-methyl erythritol 4-phosphate cytidylyltransferase; D009713:Nucleotidyltransferases; D019747:Aldose-Ketose Isomerases; D008798:Mevalonic Acid; D000077194:Chlorophyll A