Optimum conditions for transformed Panax ginseng hairy roots in flask culture.
Panax ginseng hairy roots were transformed by Agrobacterium rhizogenes KTCT 2744. They showed an active branching pattern and fast growth in hormone-free medium, and good growth at 23 degrees C, pH 5.8, 1/2 MS medium, and 3% sucrose. Sucrose provided the highest growth among seven carbon sources tested. Six complex media were also tested. In the combined sugar study, hairy roots grew better on sucrose without glucose or fructose than with glucose or fructose. In the 1/2 MS basal medium, 30 mM in nitrogen and 0.62 mM phosphate salt concentration was the optimum. The growth ratio was maximal at an inoculum size of 0.4% (w/v). Crude saponin and polysaccharide levels were also measured.
Jeong
Gwi-Taek
GT
Faculty of Chemical Engineering, Chonnam National University, Kwangju, Korea.
Park
Don-Hee
DH
Ryu
Hwa-Won
HW
Lee
Woo-Tai
WT
Park
Kyungmoon
K
Kang
Choon-Hyoung
CH
Hwang
Baik
B
Woo
Je-Chang
JC
D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D003470:Culture Media; D007700:Kinetics; D007785:Lactose / Q000378:metabolism; D009005:Monosaccharides / Q000378:metabolism; D005894:Panax / Q000254:growth & development* / Q000382:microbiology; D010710:Phosphates / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development* / Q000382:microbiology; D011134:Polysaccharides / Q000378:metabolism; D012231:Rhizobium / Q000378:metabolism*; D012503:Saponins / Q000302:isolation & purification / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D003470:Culture Media; D009005:Monosaccharides; D010710:Phosphates; D011134:Polysaccharides; D012503:Saponins; D013395:Sucrose; D007785:Lactose