[Protective effects of growth hormone en cell cultures of the central nervous system].
OBJECTIVE
We examine a possible protective effect from growth hormone (GH) against radiation in cell cultures of rat embryon brain cortex.


METHODS
Brain cortexes were removed from rat embryos of 17 days development and prepared for cell culture. The culture medium of half the plates was enriched with 500 ng/ml GH. All plates received a radiation dose of 3 Gy per plate and were again incubated for 24 hours. The TUNEL technique was employed to verify cell apoptosis in the irradiated plates and compare its level in plates with and without GH.


CONCLUSIONS
We observed that irradiated cultures with GH had significantly less cell apoptosis than those without GH and concluded that this hormone excercised a protective effect on the cell cultures. The present study demonstrated the protective effect from GH in rat embryonary cells and suggests the need for future in vitro and in vivo studies using central nervous system cells.
Isla
A
A
Servicio de Neurocirug a; Hospital General Universitario La Paz, Madrid, 28046, Espa a. eislapa@mixmail.com
Budke
M
M
Cacicedo
L
L
García-Grande
A
A
Vázquez-Rodríguez
I
I
De Miguel
E
E
Pérez-Alvarez
M
M
D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000166:cytology* / Q000187:drug effects* / Q000528:radiation effects; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000502:physiology; D013006:Growth Hormone / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling / Q000295:instrumentation; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D018696:Neuroprotective Agents; D013006:Growth Hormone