Cloning of a cation efflux pump gene associated with chlorhexidine resistance in Klebsiella pneumoniae.
Expression libraries of a chlorhexidine-resistant Klebsiella pneumoniae strain were constructed and transformed into Escherichia coli XLOLR. Twenty chlorhexidine-resistant transformants were obtained after selection. All clones contained a novel 903-nucleotide locus. Its sequences were compatible with a cation efflux pump, and the locus was thus designated as cepA. Retransformation using cepA-containing plasmids conferred chlorhexidine resistance to both XLOLR and a chlorhexidine-sensitive K. pneumoniae strain. Therefore, CepA is associated with chlorhexidine resistance and may act as a cation efflux pump.
Fang
Chi-Tai
CT
Department of Internal Medicine, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
Chen
Haur-Chuan
HC
Chuang
Yi-Ping
YP
Chang
Shan-Chwen
SC
Wang
Jin-Town
JT
D000595:Amino Acid Sequence; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001483:Base Sequence; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D002710:Chlorhexidine / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics
D000891:Anti-Infective Agents, Local; D027682:Cation Transport Proteins; D004269:DNA, Bacterial; D002710:Chlorhexidine