Characterization of the yeast copper-inducible promoter system in Arabidopsis thaliana.
Inducible promoters or gene-switches are used to both spatially and temporally regulate gene expression. Such regulation can provide information concerning the function of a gene in a developmental context as well as avoid potential harmful effects due to overexpression. A gfp construct under the control of a copper-inducible promoter was introduced into Arabidopsis thaliana (L.) Heynh. and the regulatory parameters of this inducible promoter were determined. Here, we describe the time-course of up- and down-regulation of GFP expression in response to copper level, the optimal regulatory levels of copper, and the tissue specificity of expression in three transgenic lines. We conclude that the copper-inducible promoter system may be useful in regulating the time and location of gene expression in A. thaliana.
Granger
C L
CL
U.S. Department of Agriculture-Agricultural Research, Service Corn Insect and Crop Genetics Research Unit, Department of Agronomy, Iowa State University, Ames, IA 50011, USA.
Cyr
R J
RJ
D016960:Agrobacterium tumefaciens; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D019327:Copper Sulfate / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D049452:Green Fluorescent Proteins; D007613:Kanamycin Resistance / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013997:Time Factors; D015003:Yeasts
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D019327:Copper Sulfate