title: Characterization of phi12, a bacteriophage related to phi6: nucleotide sequence of the large double-stranded RNA.
abstract: The isolation of additional bacteriophages besides phi6 containing segmented double-stranded RNA genomes (dsRNA) has expanded the Cystoviridae family to nine members. Comparing the genomic sequences of these viruses has allowed evaluation of important genetic as well as structural motifs. These comparative studies are resulting in greater understanding of viral evolution and the role played by genetic and structural variation in the assembly mechanisms of the cystoviruses. In this regard, the large double-stranded RNA genomic segment of bacteriophage phi12 was copied as cDNA and its nucleotide sequence determined. This genome's organization is similar to that of the large segment of bacteriophages phi6, phi8, and phi13. In the amino acid sequence of the viral RNA-dependent RNA polymerase (P2), similarity was found to the comparable proteins of phi6, phi8, and phi13. Amino acid sequence similarity was also noted in the nucleotide triphosphate phosphorylase (P4) to the comparable proteins of phi8 and phi13.
authors.affiliation: Department of Microbiology and Immunology, The Sophie Davis School of Biomedical Education, The City College of New York, New York, NY 10031, USA. pgottl@med.cuny.edu
authors.forename: Paul
authors.initials: P
authors.lastname: Gottlieb
authors.forename: Christiaan
authors.initials: C
authors.lastname: Potgieter
authors.forename: Hui
authors.initials: H
authors.lastname: Wei
authors.forename: Igor
authors.initials: I
authors.lastname: Toporovsky
mesh_terms: D000595:Amino Acid Sequence; D017126:Bacteriophage phi 6 / Q000145:classification / Q000235:genetics*; D001435:Bacteriophages / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D017910:Cystoviridae / Q000235:genetics; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins