Failure of commercial ligase chain reaction to detect Mycobacterium tuberculosis DNA in sputum samples from a patient with smear-positive pulmonary tuberculosis due to a deletion of the target region.
We report on a strain of Mycobacterium tuberculosis with a deletion in the protein antigen B gene overlapping the probe binding sites for the Abbott Diagnostics LCx M. tuberculosis (LCx-MTB) probe assay. A false-negative result with the LCx-MTB assay delayed a laboratory diagnosis of tuberculosis.
Gilpin
Christopher M
CM
Mycobacterium Reference Laboratory, Division of Microbiology, The Prince Charles Hospital, Chermside 4032, Australia. Chris_Gilpin@health.qld.gov.au
Dawson
David J
DJ
O'Kane
Gabrielle
G
Armstrong
John G
JG
Coulter
Christopher
C
D000942:Antigens, Bacterial / Q000235:genetics; D001431:Bacteriological Techniques; D011088:DNA Ligases; D004269:DNA, Bacterial / Q000032:analysis; D003951:Diagnostic Errors; D005188:False Negative Reactions; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D017384:Sequence Deletion; D013183:Sputum / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic; C065517:protein antigen b, Mycobacterium; D011088:DNA Ligases