title: Penetration of an antibacterial dentine-bonding system into demineralized human root dentine in vitro.
abstract: In this study, the penetration of three proprietary dentine-bonding agents (Prime & Bond 2.1, Single Bond, Liner Bond 2) and experimental dentine-bonding systems incorporating an antibacterial monomer, 12-methacryloyloxydodecylpyridinium bromide (MDPB), into artificial root caries lesions was evaluated, and the bactericidal activity of each material against Streptococcus mutans or Lactobacillus casei impregnated into demineralized dentine blocks was assessed. All of the commercial dentine-bonding agents were capable of penetrating into the artificial carious lesions to more than 150 microm. The depth of penetration of the experimental systems, which were based on Liner Bond 2, was not significantly different from that of their parent product. Liner Bond 2 primer exhibited the greatest bactericidal effects among the three proprietary dentine-bonding agents tested. Bactericidal activities of experimental primers containing MDPB were greater than those of any other products, and the application of 4% MDPB-containing primer resulted in complete killing of bacteria in demineralized dentine. The results indicate that the penetration of dentine-bonding agents into extensively demineralized root dentine is possible in vitro, and the experimental dentine-bonding systems containing the antibacterial monomer MDPB are capable of killing bacteria within demineralized dentine. This could be of benefit when managing root caries lesions.
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Suita, Japan. imazato@dent.osaka-u.ac.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Imazato
authors.forename: Angus W G
authors.initials: AW
authors.lastname: Walls
authors.forename: Akiko
authors.initials: A
authors.lastname: Kuramoto
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ebisu
mesh_terms: D000096:Acetone / Q000493:pharmacokinetics; D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000493:pharmacokinetics* / Q000494:pharmacology / Q000627:therapeutic use; D017438:Bisphenol A-Glycidyl Methacrylate / Q000493:pharmacokinetics; D002327:Cariostatic Agents / Q000493:pharmacokinetics* / Q000494:pharmacology / Q000627:therapeutic use; D016009:Chi-Square Distribution; D003804:Dentin / Q000378:metabolism*; D003806:Dentin Permeability; D017220:Dentin-Bonding Agents / Q000493:pharmacokinetics*; D006801:Humans; D007778:Lactobacillus / Q000187:drug effects; D008689:Methacrylates / Q000493:pharmacokinetics; D011109:Polymethacrylic Acids / Q000493:pharmacokinetics; D011726:Pyridinium Compounds / Q000493:pharmacokinetics* / Q000494:pharmacology / Q000627:therapeutic use; D017213:Root Caries / Q000188:drug therapy / Q000378:metabolism*; D013295:Streptococcus mutans / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D002327:Cariostatic Agents; C112274:Clearfil Liner Bond 2; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011726:Pyridinium Compounds; C089392:methacryloyloxydodecylpyridinium bromide; C444444:prime and bond 2.1; C115612:single bond; D000096:Acetone; D017438:Bisphenol A-Glycidyl Methacrylate