Characteristics of lysophosphatidylcholine in its inhibition of taurine uptake by human intestinal Caco-2 cells.
The characteristics of lysophosphatidylcholine (LPC) in its inhibition of the taurine uptake by human intestinal Caco-2 cells were investigated. By treating the cells with 200 microM of LPC, the taurine uptake was rapidly decreased by approximately 60%. This decrease was accompanied by an increase in the Km value for the uptake. A rapid uptake of LPC itself by the cells was also observed. The inhibitory activity of LPC was specific to the uptake of taurine and certain amino acids, while the uptake of glucose, glutamic acid and peptide (glycylglutamine) was not affected by LPC. The activity was dependent on the structure of a polar head and the bound fatty acid. The phosphorylcholine residue was likely to have played an important role, and surface active LPC with fatty acids of C14 or longer was highly inhibitory. These results suggest that the interaction of LPC with the taurine transporter in the intestinal cell membrane was the cause of the reduced taurine uptake.
Ishizuka
Koji
K
Department of Applied Biological Chemistry, The University of Tokyo, Japan.
Miyamoto
Yusei
Y
Satsu
Hideo
H
Sato
Ryuichiro
R
Shimizu
Makoto
M
D001692:Biological Transport / Q000187:drug effects; D018938:Caco-2 Cells; D003470:Culture Media; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D013500:Surface Tension; D013654:Taurine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013656:Taurocholic Acid / Q000494:pharmacology
D003470:Culture Media; D008244:Lysophosphatidylcholines; D013654:Taurine; D013656:Taurocholic Acid