Inhibition of bacterial adhesion and salmonella infection in BALB/c mice by sialyloligosaccharides and their derivatives from chicken egg yolk.
The effects of an egg-yolk-derived sialyloligosaccharide (YDS), asialo-YDS, and a sialylglycopeptide of YDS (SGP) on bacterial adhesion to intestinal epithelial cells and on Salmonella infection in BALB/c mice were examined. YDS, its derivative asialo-YDS, and SGP strongly inhibited the binding of Salmonella enteritidis but not E. coli K-88 to a human epithelial cell line, Caco-2. In a Salmonella infection experiment using BALB/c mice, oral administration of these reagents effectively prevented the bacteria from proliferating in spleen, as well as preventing lethality. An experiment using radioactive SGP orally administered to mice revealed that the compound was absorbed from the intestine into blood and eliminated via urine within 8 h. However, these reagents did not influence the production of TNF-alpha or NO. in culture macrophages. The results suggest that they inhibit Salmonella infection not by activating macrophages but by inhibiting the entry of bacteria through the gut, suggesting that YDS and its derivatives are useful for preventing Salmonella infection when ingested continuously.
Sugita-Konishi
Yoshiko
Y
Department of Biomedical Food Research and Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.
Sakanaka
Senji
S
Sasaki
Ken
K
Juneja
Lekh Raj
LR
Noda
Tetsuji
T
Amano
Fumio
F
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D018938:Caco-2 Cells; D002460:Cell Line; D002645:Chickens; D004530:Egg Yolk / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D009844:Oligosaccharides / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D012480:Salmonella Infections / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013154:Spleen / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D009844:Oligosaccharides; D014409:Tumor Necrosis Factor-alpha; C016735:sialooligosaccharides; D009569:Nitric Oxide