title: Sequencing-based typing of HLA-B*51 alleles and the significant association of HLA-B*5101 and -B*5108 with Behçet's disease in Greek patients.
abstract: Behçet's disease (BD) is widely known to be strongly associated with human leukocyte antigen (HLA) B51 in many different ethnic groups.Recently, HLA-B51 allele typing of Greek BD patients was performed to study the distribution of B*5101-B*5107 alleles in this Greek population, the B51 antigen strongly associated with BD was found to be predominantly encoded by allele B*5101. As it is now known that the B51 antigen can be encoded by 21 alleles, B*5101-B*5121, we performed HLA-B*51 allele genotyping among 58 Greek patients with BD. After serological HLA typing, typing of HLA-B*51 alleles was performed using the polymerase chain reaction-sequencing-based typing (PCR-SBT) method. The frequency of the B51 antigen was found to be significantly higher in the patient group as compared with the control group (75.9% of patients vs 22.0% of controls. In the genotyping of B51 alleles, 34 out of 44 B51-positive patients possessed B*5101, 13 out of the 44 carried B*5108. In contrast, all of the 9 B51-positive normal controls carried B*5101. This study revealed a strong association between Greeks with BD, both B*5101, B*5108, provided important insights into the molecular mechanism underlying the association between HLA status, this disease.
authors.affiliation: Department of Ophthalmology, Yokohama City University School of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Mizuki
authors.forename: M
authors.initials: M
authors.lastname: Ota
authors.forename: Y
authors.initials: Y
authors.lastname: Katsuyama
authors.forename: K
authors.initials: K
authors.lastname: Yabuki
authors.forename: H
authors.initials: H
authors.lastname: Ando
authors.forename: T
authors.initials: T
authors.lastname: Shiina
authors.forename: G D
authors.initials: GD
authors.lastname: Palimeris
authors.forename: E
authors.initials: E
authors.lastname: Kaklamani
authors.forename: D
authors.initials: D
authors.lastname: Ito
authors.forename: S
authors.initials: S
authors.lastname: Ohno
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Inoko
mesh_terms: D000483:Alleles; D001528:Behcet Syndrome / Q000208:ethnology / Q000235:genetics*; D006115:Greece / Q000208:ethnology; D015235:HLA-B Antigens / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D059949:HLA-B51 Antigen; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D015235:HLA-B Antigens; D059949:HLA-B51 Antigen