title: Hadamard transform time-of-flight mass spectrometry: a high-speed detector for capillary-format separations.
abstract: This work demonstrates that with an intrinsic duty cycle of 50% and spectral storage speeds up to 277 spectra s(-1) Hadamard transform time-of-flight mass spectrometry (HT-TOFMS) is a promising detector for any capillary-format separation that can be coupled to MS by electrospray ionization. Complete resolution of the components of a nine-peptide standard was achieved by coupling pressurized-capillary electrophoresis (pCE) to HT-TOFMS. The addition of pressure to the separation capillary decreased analysis times and stabilized the electrospray ionization source. Pulsed-pressurized injection of reserpine was used to experimentally simulate narrower peaks than those obtained in the pCE. HT-TOFMS was able to sample peaks having widths in the millisecond range.
authors.affiliation: Department of Chemistry, Stanford University, California 94305-5080, USA.
authors.forename: Facundo M
authors.initials: FM
authors.lastname: Fernández
authors.forename: José M
authors.initials: JM
authors.lastname: Vadillo
authors.forename: Joel R
authors.initials: JR
authors.lastname: Kimmel
authors.forename: Magnus
authors.initials: M
authors.lastname: Wetterhall
authors.forename: Karin
authors.initials: K
authors.lastname: Markides
authors.forename: Nestor
authors.initials: N
authors.lastname: Rodriguez
authors.forename: Richard N
authors.initials: RN
authors.lastname: Zare
mesh_terms: D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods / Q000592:standards; D016247:Information Storage and Retrieval; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D013997:Time Factors
chemical_list: D010455:Peptides