The nuclear export signal (NES) found in the amino-terminal region of carp MEK1 and MKK6 is lacking in carp MKK4.
Carp MKK4 (cMKK4) cDNA was isolated from an ovary cDNA library. cMKK4 mRNA was ubiquitously distributed in various tissues of adult carp. Sequence analysis revealed that cMKK4 lacks a nuclear export signal sequence, unlike mammalian and frog MEKs (ERK activator) and cMKK6 (carp p38 activator), where it plays an important role in anchoring these MAP kinase activators to the cytoplasm. cMKK4 protein was found to be diffused throughout the cell, whereas cMEK1 and cMKK6 proteins were seen exclusively in the cytoplasm.
Hashimoto
Hisashi
H
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Matsuo
Yoshiyuki
Y
Yokoyama
Yoshihiro
Y
Toyohara
Haruhiko
H
Sakaguchi
Morihiko
M
D021581:Active Transport, Cell Nucleus / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics* / Q000378:metabolism; D002347:Carps / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D048369:MAP Kinase Kinase 1; D048669:MAP Kinase Kinase 6; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017346:Protein Serine-Threonine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048369:MAP Kinase Kinase 1; D048669:MAP Kinase Kinase 6; D020929:Mitogen-Activated Protein Kinase Kinases