Spatially resolved IR microspectroscopy of single cells.
Fourier transform IR (FTIR) microspectroscopy at a spatial resolution of 18 microm was used to study skin fibroblasts and giant sarcoma cells. Both cell lines were derived from the same patient; they were metabolically active and in the exponentially growing phase. The IR spectra were acquired for the nuclei and cytosol of untreated cells, cells washed with ethanol, and cells treated with RNase or DNase. A comparison of the spectra of the two cell lines yielded only insignificant spectral differences, indicating that IR spectroscopy monitors the overall cell activity rather than specific signs of cancer.
Lasch
Peter
P
Department of Chemistry, Hunter College and Graduate Center of the City University of New York, 695 Park Avenue, New York, New York 10021, USA. laschp@rki.de
Pacifico
Anthony
A
Diem
Max
M
D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D015726:Giant Cells / Q000166:cytology; D006801:Humans; D012260:Ribonucleases / Q000378:metabolism; D012509:Sarcoma / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D012997:Solvents / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D012997:Solvents; D000431:Ethanol; D003851:Deoxyribonucleases; D012260:Ribonucleases