title: Sensitive and rapid liquid chromatography-tandem mass spectrometry method for the determination of stavudine in human plasma.
abstract: A sensitive method for the determination of stavudine in plasma was developed, using high-performance liquid chromatographic separation with tandem mass spectrometric detection. The samples were extracted from plasma with Waters, Sep-Pak Vac, 100 mg, tC(18) solid-phase extraction (SPE) columns. Chromatography was performed on a Supelco Discovery C(18), 5 microm, 150 x 2 mm column with a mobile phase consisting of ammonium acetate (0.01 M)-acetonitrile-methanol (800:100:100, v/v/v) at a flow-rate of 0.3 ml/min. Detection was achieved by an Applied Biosystems API 2000 mass spectrometer (LC-MS-MS) set at unit resolution in the multiple reaction monitoring mode (MRM). Atmospheric pressure chemical ionization (APCI) was used for ion production. The mean recovery for stavudine was 94% with a lower limit of quantification set at 4 ng/ml. This assay method makes use of the increased sensitivity and selectivity of mass spectrometric (MS-MS) detection to allow for a more rapid (extraction and chromatography) and selective method for the determination of stavudine in human plasma than has previously been described.
authors.affiliation: FARMOVS-PAREXEL Clinical Research Organisation, Private Bag X09, Brandhof, Bloemfontein 9324, South Africa. lubbe.wiesner@farmovs-parexel.com
authors.forename: J L
authors.initials: JL
authors.lastname: Wiesner
authors.forename: F C W
authors.initials: FC
authors.lastname: Sutherland
authors.forename: M J
authors.initials: MJ
authors.lastname: Smit
authors.forename: G H
authors.initials: GH
authors.lastname: van Essen
authors.forename: H K L
authors.initials: HK
authors.lastname: Hundt
authors.forename: K J
authors.initials: KJ
authors.lastname: Swart
authors.forename: A F
authors.initials: AF
authors.lastname: Hundt
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D018894:Reverse Transcriptase Inhibitors / Q000097:blood* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D018119:Stavudine / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D018894:Reverse Transcriptase Inhibitors; D018119:Stavudine