[Methanotrophic communities in the soils of Russian northern taiga and subarctic tundra].
The PCR analysis of DNA extracted from soil samples taken in Russian northern taiga and subarctic tundra showed that the DNA extracts contain genes specific to methanotrophic bacteria, i.e., the mmoX gene encoding the conserved alpha-subunit of the hydroxylase component of soluble methane monooxygenase, the pmoA gene encoding the alpha-subunit of particulate methane monooxygenase, and the mxaF gene encoding the alpha-subunit of methanol dehydrogenase. PCR analysis with group-specific primers also showed that methanotrophic bacteria in the northern taiga and subarctic tundra soils are essentially represented by the type I genera Methylobacter, Methylomonas, Methylosphaera, and Methylomicrobium and that some soil samples contain type II methanotrophs close to members of the genera Methylosinus and Methylocystis. The electron microscopic examination of enrichment cultures obtained from the soil samples confirmed the presence of methanotrophic bacteria in the ecosystems studied and showed that the methanotrophs contain only small amounts of intracytoplasmic membranes.
Kaliuzhnaia
M G
MG
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.
Makutina
V A
VA
Rusakova
T G
TG
Nikitin
D V
DV
Khmelenina
V N
VN
Dmitriev
V V
VV
Trotsenko
Iu A
IuA
D000429:Alcohol Oxidoreductases / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008697:Methane / Q000378:metabolism*; D020580:Methylobacterium / Q000235:genetics / Q000302:isolation & purification; D020620:Methylomonas / Q000235:genetics / Q000302:isolation & purification; D020581:Methylosinus / Q000235:genetics / Q000302:isolation & purification; D008854:Microscopy, Electron; D010105:Oxygenases / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D012988:Soil Microbiology
D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); D010105:Oxygenases; C021555:methane monooxygenase; D008697:Methane