The effect of increased dietary intake on superovulatory response to FSH in heifers.
We have previously shown that the number of ovarian follicles <4 mm in diameter can be increased by enhanced dietary intake in heifers. This study investigated the effect of the same dietary treatment on superovulatory response. The estrous cycles of 24 mature Hereford x Friesian heifers were synchronized by a standard progesterone plus prostaglandin protocol. The animals were fed with either 100% (group M, n = 12) or 200% (group 2M, n = 12) maintenance requirements for a 3-week period. Starting from day 4 of the synchronized estrous cycle, all the animals were superovulated using a standard 4-day FSH regime followed by an injection of GnRH analogue (GnRHa) to induce ovulation. Rectal ultrasound scanning was carried out to assess ovarian follicular populations at the start of FSH treatment and on the day of GnRHa injection, and to determine the number of corpora lutea 5 days after GnRHa injection. The body weight (BW) and body condition score (BCS) were recorded weekly and plasma samples were collected throughout the experimental period. There were no differences in either BW or BCS between two groups at the start of the experiment. The BW and BCS were maintained during the experiment in the group M, whilst animals in the group 2M showed a non-significant (P > 0.05) increase in BW and BCS. Circulating concentrations of insulin were significantly (P < 0.01) higher in heifers from the group 2M throughout the controlled feeding period. The group 2M had significantly (P < 0.05) more follicles 2-4 mm in diameter at the start of FSH treatment and more (P < 0.01) follicles >9 mm in diameter on the day of GnRHa injection, when compared with the group M. Similarly, 5 days after GnRHa injection there were significantly (P < 0.01) more corpora lutea in the group 2M (18.1+/-2.2) than in the group M (10.6+/-3.0). In addition, plasma progesterone concentrations following GnRHa injection were significantly (P < 0.01) higher in heifers from the group 2M. In conclusion, these results confirm that increased dietary intake can enhance the recruitment of ovarian follicles in heifers. This treatment may provide a valuable approach to improving superovulatory response in cattle.
Gong
J G
JG
Division of Integrative Biology, Roslin Institute, Edinburgh, Midlothian, UK. jin-gui.gong@bbsrc.ac.uk
Armstrong
D G
DG
Baxter
G
G
Hogg
C O
CO
Garnsworthy
P C
PC
Webb
R
R
D000818:Animals; D001823:Body Composition; D001835:Body Weight; D002064:Buserelin / Q000008:administration & dosage; D002417:Cattle / Q000502:physiology*; D003008:Cloprostenol / Q000008:administration & dosage; D003338:Corpus Luteum / Q000033:anatomy & histology; D004032:Diet; D015237:Dinoprost / Q000008:administration & dosage; D004973:Estrus Synchronization; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D006080:Ovarian Follicle / Q000033:anatomy & histology; D010060:Ovulation; D011374:Progesterone / Q000008:administration & dosage / Q000097:blood; D013480:Superovulation
D007328:Insulin; D003008:Cloprostenol; D011374:Progesterone; D005640:Follicle Stimulating Hormone; D015237:Dinoprost; D002064:Buserelin