title: Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from water samples.
abstract: OBJECTIVE
  Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from tap water.
  
  
  RESULTS
  Ferric sulphate, aluminium sulphate and calcium carbonate were compared for their recovery efficiency of C. parvum oocysts from tap water. Lower mean recovery was achieved by calcium carbonate (38.8%) compared with ferric sulphate (61.5%) and aluminium sulphate (58.1%) for the recovery of 2.5 x 10(5) oocysts l(-1); 2.5 oocysts l(-1) and 1 oocyst l(-1) were adequately purified using ferric sulphate flocculation. In vitro excystation experiments showed that ferric sulphate flocculation does not markedly reduce the viability of oocysts.
  
  
  CONCLUSIONS
  Ferric sulphate flocculation is a simple and effective tool for the purification of C. parvum oocysts from tap water.
  
  
  CONCLUSIONS
  The high recovery rates and low impact on oocyst viability provided by ferric sulphate flocculation might be useful for the detection of Cryptosporidium oocysts in environmental water samples.
authors.affiliation: Institute for Medical Parasitology, Medical Faculty, University of Bonn, Germany. Panagiotis.Karanis@medizin.uni-koeln.de
authors.forename: P
authors.initials: P
authors.lastname: Karanis
authors.forename: A
authors.initials: A
authors.lastname: Kimura
mesh_terms: D000534:Alum Compounds / Q000737:chemistry*; D000818:Animals; D002119:Calcium Carbonate / Q000737:chemistry*; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D005290:Ferric Compounds / Q000737:chemistry*; D005428:Flocculation Tests / Q000379:methods; D014871:Water Microbiology
chemical_list: D000534:Alum Compounds; D005290:Ferric Compounds; C041524:aluminum sulfate; C024823:ferric sulfate; D002119:Calcium Carbonate