Tiny telomere DNA.
We describe the design, synthesis and biophysical characterization of a novel DNA construct in which a folded quadruplex structure is joined to a standard double helix. Circular dichroism, gel electrophoresis, three-dimensional UV melting and differential scanning calorimetry were all used to characterize the structure. Rigorous molecular dynamics simulations were used to build a plausible atomic-level structural model of the DNA construct. This novel DNA construct provides a model for the duplex-quadruplex junction region at the end of chromosomal DNA and offers a system for the study of structure-selective ligand binding.
Ren
Jinsong
J
Department of Biochemistry, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216-4505, USA.
Qu
Xiaogang
X
Trent
John O
JO
Chaires
Jonathan B
JB
D001483:Base Sequence; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D054856:G-Quadruplexes; D008024:Ligands; D008958:Models, Molecular; D009690:Nucleic Acid Conformation / Q000528:radiation effects; D009691:Nucleic Acid Denaturation / Q000528:radiation effects; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D016615:Telomere / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D013816:Thermodynamics; D014466:Ultraviolet Rays
D008024:Ligands; D009838:Oligodeoxyribonucleotides; D004247:DNA