title: A new simple and rapid electrochemical method for the determination of hydroxyl radical generated by Fenton reaction and its application.
abstract: A simple and rapid electrochemical method to detect the hydroxyl radical is described. This method employed the reaction between.OH and dimethyl sulfoxide (DMSO) to generate quantitatively formaldehyde, which then reacted with hydrazine hydrochloride at pH 6.2. The product showed a second-order derivative cathodic wave with the peak potential of -1.08 V( vsSCE) by single sweep oscillopolarography. The electrochemical behavior of the product was investigated by single sweep oscillopolarography and cyclic voltammetry. The experimental conditions for the measurement were optimized and the scavenging activity of some flavonoids on hydroxyl radicals was studied.
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100037, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zou
authors.forename: Chao
authors.initials: C
authors.lastname: Tai
authors.forename: Xue-Xin
authors.initials: XX
authors.lastname: Gu
authors.forename: Ruo-Hua
authors.initials: RH
authors.lastname: Zhu
authors.forename: Qi-Hua
authors.initials: QH
authors.lastname: Guo
mesh_terms: D001205:Ascorbic Acid; D004492:Edetic Acid; D004563:Electrochemistry / Q000379:methods; D005557:Formaldehyde / Q000032:analysis; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical / Q000032:analysis*; D007202:Indicators and Reagents; D007501:Iron; D007700:Kinetics; D011048:Polarography / Q000379:methods
chemical_list: C045076:Fenton's reagent; D007202:Indicators and Reagents; D005557:Formaldehyde; D017665:Hydroxyl Radical; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D007501:Iron; D001205:Ascorbic Acid