[Construction and expression of a vector containing protein transduction domain and bcr/abl fusion gene].
OBJECTIVE
To construct a vector containing protein transduction domain (PTD) and bcr/abl fusion gene of chronic myelogenous leukemia and express PTD-bcr/abl fusion protein in E. Coli.


METHODS
DNA fragment encoding PTD was synthesized and fused to PCR-amplified bcr/abl gene fragment, then inserted into plasmid pET-16b to get the expression vector pEPb containing PTD-bcr/abl fusion gene, which was transfected and expressed in E. Coli LB21. PTD-bcr/abl fusion protein was purified by affinity chromatography.


RESULTS
523 bp bcr/abl fusion gene was effectively amplified. The PTD-bcr/abl gene sequencing showed the same sequence as scheduled. The fusion peptide was successfully expressed in E. Coli and purified.


CONCLUSIONS
The results may provide a new PTD-bcr/abl fusion peptide for the immunotherapy of CML.
Liang
Yingmin
Y
Department of Hematology of Tangdu Hospital and Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi'an 710038, China.
Sun
Qiang
Q
Jiang
Shanshan
S
Wang
Jizhu
J
Wu
Rongli
R
Chen
Ping
P
Liu
Li
L
Han
Hua
H
D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D016044:Fusion Proteins, bcr-abl / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D015696:Gene Products, tat / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D016044:Fusion Proteins, bcr-abl