[Characterization of pathogenic bacteria of the cupped oyster Crassostrea gigas].
The French mollusc production is mainly based on the Pacific cupped oyster, Crassostrea gigas. Since 1991, outbreaks of mass mortality of juveniles are reported during the summer period. These outbreaks are a major concern of oyster industry. Several studies have established given bacterial strains to be pathogenic for bivalve species, including oysters. Here we present a study of mortality outbreaks of C. gigas, as initiated in 1995. In a first step, bacterial strains were isolated during mass mortality outbreak and were biochemically characterised. Among the isolated strains, some strains of Vibrio splendidus biovar II were found to be pathogenic by means of experimental challenge of oyster juveniles. In the second step, a genotypical identification of the pathogenic strain was undertaken, based on 16S RNA sequences and phylogenetic analysis. It confirmed that the pathogenetic strain belonged to Vibrio splendidus biovar II.
Waechter
Magali
M
Laboratoire de génétique et pathologie, Ifremer, 17390 La Tremblade, France.
Le Roux
Frédérique
F
Nicolas
Jean-Louis
JL
Marissal
Eric
E
Berthe
Franck
F
D000818:Animals; D005838:Genotype; D010125:Ostreidae / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D014733:Vibrio / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S