Screening An alpha-glucosidase Inhibitor from A Phage-displayed Peptide Library.
A phage-displayed peptide library was biopanned with Aspergillus niger glucoamylase. Three specifically binding phage clones (GB-1, GB-2 and GB-3) were identified after three cycles of screening and amplification. All three peptides contained a disulfide bridge, and the impairment of disulfide bond greatly lowered the binding activity. One peptide, cyclic KCHFEECLAY, representing the N-terminal 10 amino acid residues from GB-1, competitively inhibited A. niger glucoamylase (K(I) 0.2 mmol/L) as well as rat intestinal alpha-glucosidase (KI 1.4 mmol/L).
Li
Jia-Da
JD
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China. kyw0717@sunm.shcnc.ac.cn
Wang
Ke-Yi
KY