title: Effect of caries disclosing agents on bond strengths of total-etch and self-etching primer dentin bonding systems to resin composite.
abstract: This study examined the effect of caries disclosing dyes on composite to dentin shear bond strengths of a total etch, one-bottle and two self-etching, non-rinsing primer dental adhesives. Two caries disclosing dyes were evaluated, Seek and Snoop, with three dentin adhesives, Prime & Bond NT, Prompt L-Pop and Clearfil SE Bond. Extracted human molars stored in 0.2% sodium azide were sectioned longitudinally to expose dentin and embedded in acrylic, leaving the dentin exposed. Each dentin adhesive had three test groups (n=12); a control and one with each of the caries disclosing dyes. The control group had the dentin conditioned and the adhesive applied following the manufacturer's instructions. The caries disclosing dye groups had the dentin first treated for 10 seconds with the disclosing dye, rinsed, then the dentin adhesives were applied as in the controls. A column of Tetric Ceram was bonded after dentin adhesive placement to each specimen and light cured. Specimens were stored in room temperature water for 24 hours, thermocycled for 1,000 cycles between 5 degrees C and 55 degrees C and tested in shear until failure. Mean +/- SD shear bond values (SBV) were determined in MPa. A one-way ANOVA and Student Neuman Keuls multiple comparison test within each DBS were performed at a significance level ofp<0.05 to analyze the caries disclosing dyes input on SBV versus the controls. Surface analysis to determine the nature of the type of dentin/composite fracture/ separation was also performed. For the fracture analysis data, a Chi-Square test was performed at a significance level of p<0.05. The results of this study indicate that using the two tested caries disclosing dyes, with a total etch, one bottle and two self-etching, non-rinsing primer dental adhesives did not negatively affect the dentin-to-composite shear bond strengths of the three tested dentin bonding systems (p>0.05).
authors.affiliation: Preclinical Operative Dentistry Courses, University of Connecticut Health Center, USA. kazemi@nso2.uchc.edu
authors.forename: Reza B
authors.initials: RB
authors.lastname: Kazemi
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Meiers
authors.forename: Karim
authors.initials: K
authors.lastname: Peppers
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000704:Analysis of Variance; D016009:Chi-Square Distribution; D004396:Coloring Agents / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry*; D001840:Dental Bonding; D003731:Dental Caries / Q000175:diagnosis*; D003804:Dentin / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D011109:Polymethacrylic Acids / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D013223:Statistics as Topic; D013314:Stress, Mechanical; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: C438313:Clearfil SE Bond; D004396:Coloring Agents; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; C408584:Prompt L-Pop; D019279:Resin Cements; C121818:Tetric ceram; D014867:Water