title: Chromosomal ampC genes in Enterobacter species other than Enterobacter cloacae, and ancestral association of the ACT-1 plasmid-encoded cephalosporinase to Enterobacter asburiae.
abstract: The amplification and sequence of ampC genes in Enterobacter asburiae, Enterobacter cancerogenus, Enterobacter dissolvens, Enterobacter hormaechei and Enterobacter intermedius bring the number of known cephalosporinase sequences from the genus Enterobacter to seven. Expression in Escherichia coli of the ampC genes from E. asburiae, E. hormaechei and E. intermedius established the functional nature of these genes. ampC from E. asburiae shows 96.5% identity to bla(ACT-1) encoding a plasmid-borne cephalosporinase previously believed to derive from Enterobacter cloacae. The reassignment of ACT-1 ancestry to E. asburiae is confirmed by the 95.5% identity between ampR upstream of bla(ACT-1) and ampR from E. asburiae.
authors.affiliation: Service de Bactériologie, Hôpital Tenon, UFR Saint Antoine, Paris, France. martin.rottman@rpc.ap-hop-paris.fr
authors.forename: Martin
authors.initials: M
authors.lastname: Rottman
authors.forename: Yahia
authors.initials: Y
authors.lastname: Benzerara
authors.forename: Béatrice
authors.initials: B
authors.lastname: Hanau-Berçot
authors.forename: Chantal
authors.initials: C
authors.lastname: Bizet
authors.forename: Alain
authors.initials: A
authors.lastname: Philippon
authors.forename: Guillaume
authors.initials: G
authors.lastname: Arlet
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins; D001483:Base Sequence; D002510:Cephalosporinase / Q000235:genetics*; D002876:Chromosomes, Bacterial; D017931:DNA Primers; D004754:Enterobacter / Q000145:classification / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D016972:Enterobacter cloacae / Q000235:genetics*; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D010957:Plasmids; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D017931:DNA Primers; D047090:beta-Lactams; D002510:Cephalosporinase; C048795:AmpC beta-lactamases; D001618:beta-Lactamases