title: Promoter elements in the aflatoxin pathway polyketide synthase gene.
abstract: PksA catalyzes the formation of the polyketide backbone necessary for aflatoxin biosynthesis. Based on reporter assays and sequence comparisons of the nor1-pksA intergenic region in different aflatoxin-producing Aspergillus species, cis-acting elements for the aflatoxin pathway-specific regulatory protein, AflR, and the global-acting regulatory proteins BrlA and PacC are involved in pksA promoter activity.
authors.affiliation: Southern Regional Research Center, United States Department of Agriculture, P.O. Box 19687, 1100 R.E. Lee Blvd., New Orleans, LA 70179, USA. erlich@nola.srrc.usda.gov
authors.forename: Kenneth C
authors.initials: KC
authors.lastname: Ehrlich
authors.forename: Beverly G
authors.initials: BG
authors.lastname: Montalbano
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Cary
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Cotty
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis*; D001230:Aspergillus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C113837:AFLR protein, Aspergillus; D000348:Aflatoxins; C063497:BRLA protein, Emericella nidulans; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009097:Multienzyme Complexes; D009687:Nuclear Proteins; C092394:PacC protein, Aspergillus; D014157:Transcription Factors