Apoptotic cell death of cerebellar granule cells in rolling mouse Nagoya.
Rolling mouse Nagoya is a voltage dependent calcium channel alpha1A subunit mutant showing moderate ataxia. Granule cell loss was previously reported in the cerebellar vermis of homozygous rolling. Apoptotic cerebellar granule cell death was reported in homozygous leaner mice, an allele of rolling. Cerebella of 21-day-old rolling and wild type mice were used for terminal dUTP nick-end-labeling (TUNEL) assay and electron microscopic observation to understand the mechanism of granule cell loss in rolling mice. The number of TUNEL-positive cells was significantly increased in rolling. More TUNEL-positive cells were observed in the anterior cerebellar vermis compared with posterior. Condensation and fragmentation of granule cell nuclei in rolling mouse were observed frequently. These results suggest that apoptosis is one of the mechanisms of granule cell loss in the rolling cerebellum.
Suh
Young Suk
YS
Department of Anatomy, College of Medicine and Human Genetics, Institute of Medicine Science Research Center, Korea University, 126-1 Anam-Dong 5-Ga, Sungbuk-Ku, Seoul 136-705, South Korea.
Oda
Sen ich
Si
Kang
Yoon Hee
YH
Kim
Hyun
H
Rhyu
Im Joo
IJ
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001259:Ataxia / Q000235:genetics* / Q000503:physiopathology*; D015220:Calcium Channels / Q000235:genetics; D002531:Cerebellum / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D006720:Homozygote; D020287:In Situ Nick-End Labeling; D008297:Male; D051379:Mice; D008818:Mice, Neurologic Mutants / Q000502:physiology*; D008854:Microscopy, Electron; D020033:Protein Isoforms / Q000235:genetics; D012016:Reference Values
D015220:Calcium Channels; D020033:Protein Isoforms