Fecal glucocorticoid metabolites as a measure of adrenal activity in dairy cattle.
The feasibility of monitoring acute adrenal activity in New Zealand dairy cattle by measuring fecal glucocorticoid metabolites was investigated. Fecal glucocorticoid measurement has potential as an indicator of adrenal activity and animal stress because sampling is relatively noninvasive, does not interfere with the stress response itself, and permits on-farm monitoring. Fecal samples were collected from dairy cattle following ACTH challenge and exposure to stressors (novel environment, transport). Two immunoassays (11,17-dioxoandrostane enzymeimmunoassay and ICN corticosterone radioimmunoassay) were compared. Both assays detected increased immunoreactive fecal glucocorticoid metabolites following acute adrenal activity and the temporal relationship between plasma corticosteroids and fecal metabolite excretion was determined. The time to peak excretion was closely related to the transit time of digesta passing between the bile duct and the rectum and was affected by seasonal changes in feed intake and pasture digestibility. We conclude that measurement of glucocorticoid metabolites reliably indicates acute adrenal activity in dairy cattle and in combination with other physiological and behavioral measures has potential for monitoring health and welfare in dairy cattle. (c) 2002 Elsevier Science (USA).
Morrow
Catherine J
CJ
Ruakura Research Centre, AgResearch, PB 3123, Hamilton, New Zealand. catherine.morrow@agresearch.co.nz
Kolver
Eric S
ES
Verkerk
Gwyneth A
GA
Matthews
Lindsay R
LR
D000311:Adrenal Glands / Q000502:physiology*; D000324:Adrenocorticotropic Hormone; D000731:Androstanes / Q000032:analysis; D000818:Animals; D002417:Cattle / Q000502:physiology*; D003345:Corticosterone / Q000032:analysis; D004435:Eating; D005243:Feces / Q000737:chemistry*; D005260:Female; D005772:Gastrointestinal Transit; D005938:Glucocorticoids / Q000032:analysis* / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007774:Lactation; D011247:Pregnancy; D011863:Radioimmunoassay; D012621:Seasons
D000731:Androstanes; D005938:Glucocorticoids; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone