Effect on Glucose Transport and Anion Exchange in Human Erythrocytes by Mechanical Force Factors.
The mechanical forces play an important role in both the normal physiology and the pathology of the cardiovascular system. It was observed that an increase in speed of rotary flow of the intact erythrocyte suspension resulted in increase in the rate of glucose entry across the erythrocyte membranes. The effects of osmotic stress and a membrane curvature-altering drug, chlorpromazine, on the glucose and anion transport were also shown. The decrease in the activation energy for glucose and anion transport according to Arrhenius plot for temperature dependence of membrane transport meant that thermodynamic barrier for transporter reorientation in the membranes was reduced. The data from the measurement of intrinsic fluorescence quench in ghosts indicated that the conformations of both glucose transport protein (GLUT1) and anion transport protein (band 3) in the erythrocyte membranes were affected by the mechanical force factors. After inhibition of anion transport, a modification of the response of glucose transport by the mechanical force was observed, which also indicates that there is an information linkage between GLUT1 and band 3 in the erythrocyte membranes.
Li
Yue-Zhou
YZ
Liren Laboratory, Department of Physiology and Biophysics, School of Life Sciences, Fudan University, Shanghai 200433, China. zhzhang@fudan.edu.cn
Zhou
Han-Qing
HQ
Peng
Feng
F
Zhang
Zhi-Hong
ZH