[A preliminary genetic reassortment between Hantaan virus and Seoul virus strains].
OBJECTIVE
To determine the frequency and characteristics of reassortment among Hantaan and Seoul viruses causing hemorrhagic fever with renal syndrome (HFRS).


METHODS
Mixed infections were initiated in tissue culture, using Hantaan virus strain 76 - 118 and Seoul virus strain SR-11. Potential reassortant virus plaques were picked out by multiplex RT-PCR, using primers specific for individual genome segments (L, M, S) of each strain.


RESULTS
Most of the progeny virus plaques (68.19% of 44) had parental genotype of 76 - 118 strain or SR-11 strain while 2 of 44 plaques had mixed genotypes that yielded RT-PCR bands for the same segment of both parental strains. Reassortant viruses were detected in 68.19% of 44 progeny plaques tested, involving the M and S segments. In addition, approximately 4.55% of the progeny virus plaques appeared to contain S or M segments originating from both parental virus strains, showing that they were diploid.


CONCLUSIONS
Genetic reassortment can occur between Hantaan virus and Seoul virus strains.
Kang
Wenzhen
W
Department of Infectious Diseases, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China.
Huang
Changxing
C
Bai
Xuefan
X
Yang
Weisong
W
Li
Guangyu
G
D000818:Animals; D002522:Chlorocebus aethiops; D016679:Genome, Viral; D005838:Genotype; D018063:Hantaan virus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029263:Seoul virus / Q000235:genetics*; D014709:Vero Cells
D012367:RNA, Viral