Anti-apoptotic role of phospholipase D in spontaneous and delayed apoptosis of human neutrophils.
Neutrophil apoptosis is a constitutive process that can be enhanced or delayed by signals induced by various stimuli. We investigated the role of phospholipase D (PLD) in neutrophil apoptosis. The apoptotic rate of neutrophils was found to be increased by 1-butanol and decreased by the exogenous addition of PLD. Moreover, the delay of apoptosis by apoptosis-delaying stimuli such as granulocyte/macrophage colony-stimulating factor or lipopolysaccharide (LPS) was also blocked by 1-butanol. Unstimulated PLD activity in cultured cells for 20 h was higher than that in freshly isolated cells and further increased in cultured cells with LPS. These results suggest that PLD is involved in the up-regulation of neutrophil survival.
Park
Myung Ae
MA
Department of Biochemistry, Dong-A University College of Medicine, Busan, South Korea.
Lee
Min Jung
MJ
Lee
Sang Hwa
SH
Jung
Dong Keun
DK
Kwak
Jong Young
JY
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D000440:Butanols / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D010739:Phospholipase D / Q000378:metabolism* / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects
D000319:Adrenergic beta-Antagonists; D000440:Butanols; D016209:Interleukin-8; D008070:Lipopolysaccharides; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D011433:Propranolol; D010739:Phospholipase D