Effect of rancid corn oil on some onco/suppressor gene expressions in vivo. A short-term study.
Autooxidation of polyunsaturated fatty acids (PUFAs) of edible oils results in the formation of fatty acid hydroperoxides that can undergo further chemical transformations to yield a variety of re-arranged and chain-cleavage products. Since the oxidation products of PUFAs have been reported to have cytotoxic and mutagenic effects, the consumption of rancid oils and fats represents a possible health hazard for the population. Storage of corn oil at room temperature and in the refrigerator for a forty-eight month period resulted in two different qualities of oil samples, which were characterized by UV, titrimetric (peroxide value, acid value) and GC-MS methods. Earlier it was demonstrated that the increase of expression of certain oncogenes and tumor suppressor genes is a method of choice for the early detection of carcinogen exposure. Treatment of CBA/Calpha inbred mice with the two oil samples showed significantly increased expression of the Ha-ras gene in all the investigated organs (liver, lung, kidney, thymus and spleen) of the rancid corn oil-treated animals. Expression of the c-myc and the p53 genes was also increased after the rancid corn oil-treatment in all the organs but the thymus of the mice. The results suggest that rancid oils, rich in omega-6 unsaturated fatty acids, could be involved not only in tumor promotion but in initiation as well.
Perjési
Pál
P
Department of Medical Chemistry, University Medical School of Pécs, Hungary.
Pintér
Zoltán
Z
Gyöngyi
Zoltán
Z
Ember
István
I
D000818:Animals; D003314:Corn Oil / Q000737:chemistry / Q000633:toxicity*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D016259:Genes, myc / Q000187:drug effects*; D016158:Genes, p53 / Q000187:drug effects*; D011905:Genes, ras / Q000187:drug effects*; D008054:Lipid Peroxides / Q000737:chemistry / Q000633:toxicity*; D051379:Mice; D008808:Mice, Inbred CBA; D010084:Oxidation-Reduction; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics
D008054:Lipid Peroxides; D016271:Proto-Oncogene Proteins c-myc; D016159:Tumor Suppressor Protein p53; D012313:RNA; D003314:Corn Oil; D016283:Proto-Oncogene Proteins p21(ras)