ADA3-containing complexes associate with estrogen receptor alpha.
Transcriptional repression and activation by nuclear receptors (NRs) are brought about by coregulator complexes. These complexes modify the chromatin environment of target genes and affect the activity of the basal transcription machinery. We have previously implicated the yeast ADA3 protein in transcriptional activation by estrogen and retinoid X receptors in yeast and mammalian cells. Here we report the cloning of the mouse homolog of ADA3 and its characterization with respect to the estrogen receptor alpha (ERalpha) function. Mouse mADA3 is 23% identical and 47% similar to yeast yADA3, and mADA3 in contrast to yADA3 does not interact with NRs directly even though it contains two LxxLL NR boxes. However, the ADA3-containing TBP-free-TAF-containing complex (TFTC) can interact with ERalpha in a ligand-independent manner, indicating that other subunits of the complex are sufficient to mediate interaction with NRs.
Benecke
Arndt
A
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Collège de France, BP163, 67404 Illkirch Cedex, France.
Gaudon
Claudine
C
Garnier
Jean-Marie
JM
vom Baur
Elmar
E
Chambon
Pierre
P
Losson
Régine
R
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D047628:Estrogen Receptor alpha; D020224:Expressed Sequence Tags; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011485:Protein Binding; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017385:Sequence Homology; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D047628:Estrogen Receptor alpha; D005656:Fungal Proteins; D046911:Macromolecular Substances; C083323:NGG1 protein, S cerevisiae; D011960:Receptors, Estrogen; D029701:Saccharomyces cerevisiae Proteins; C580756:Tada3 protein, mouse; D014157:Transcription Factors