Antibody cross-reactivity of alphaherpesviruses as mirrored in naturally infected primates.
The extent of antibody cross-reactivity of pooled antisera from rhesus monkeys, baboons, African green monkeys, langurs, sooty mangabeys and humans to 6 alphaherpesviruses (herpes B virus, herpes papio 2, simian agent 8, langur herpes virus and herpes simplex 1 & 2) was examined by two types of enzyme linked immunosorbent assays, an antibody capture assay (tELISA) and an antigen capture assay (dELISA). Percent cross-reactivity was calculated for each serum by comparison of the homologous reaction (100%) to the reaction with heterologous viruses. Comparison of the immunological reactivity of the mangabey antiserum pool to the panel of alphaherpesviruses indicated that these antibodies were induced by a yet unidentified alphaherpesvirus. In general, monkey sera were more cross-reactive to monkey herpesviruses than to human herpesviruses.However, the extent of cross-reactivity of monkey sera to human herpesviruses was relatively lower than the cross-reactivity of human sera to monkey herpes-viruses. Because of this phenomenon of "one-way" cross-reactivity that was also observed within the group of simian herpesviruses, it was difficult to rank the immunologic distances between the viruses in absolute terms.
Katz
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D
Viral Immunology Center, Department of Biology, Georgia State University, Atlanta, Georgia 30303, USA. biodkkx@langate.gsu.edu
Shi
W
W
Krug
P W
PW
Henkel
R
R
McClure
H
H
Hilliard
J K
JK
D018138:Alphaherpesvirinae / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D016665:Cercocebus atys / Q000276:immunology; D002520:Cercopithecidae / Q000276:immunology; D002522:Chlorocebus aethiops / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D008253:Macaca mulatta / Q000276:immunology; D010215:Papio / Q000276:immunology; D011323:Primates / Q000276:immunology*; D013045:Species Specificity
D000914:Antibodies, Viral; D007106:Immune Sera