Selective monitoring of 3 out of 50,000 protein vibrations.
An important group in a large protein, which is the phosphate group that transiently binds to the Ca2+ transporting ATPase (Ca2+-ATPase) of the sarcoplasmic reticulum membrane, is selectively observed with IR spectroscopy. The three phosphate stretching vibrations stand out from the 50,000 protein vibrations in an isotope exchange experiment where oxygen exchange at the phosphate group is catalyzed by the Ca2+-ATPase.
Barth
Andreas
A
Institut für Biophysik, Johann Wolfgang Goethe-Universität, Theodor Stern Kai 7, Haus 74, D-60590 Frankfurt am Main, Germany. barth@biophysik.uni-frankfurt.de
D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000737:chemistry / Q000378:metabolism*; D007554:Isotopes; D010100:Oxygen / Q000378:metabolism; D011506:Proteins / Q000502:physiology*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
D007554:Isotopes; D011506:Proteins; D000252:Calcium-Transporting ATPases; D010100:Oxygen; D002118:Calcium