Crystallization and preliminary X-ray crystallographic studies of the sixth PDZ domain of glutamate-receptor interacting protein 1 (GRIP1) from Rattus norvegicus.
The sixth PDZ domain from GRIP1 and its complex with the octapeptide of the liprin-alpha1 C-terminus were crystallized at 294 K by the hanging-drop vapour-diffusion method. The native crystal belongs to space group P6(1)22 (or P6(5)22), with unit-cell parameters a = b = 40.3, c = 222.9 A. The complex crystal belongs to space group R32, with unit-cell parameters a = b = 117.8, c = 102.0 A. Native and peptide-complex diffraction data were collected to resolutions of 1.5 and 1.8 A, respectively, using synchrotron X-rays.
Park
Seong Ho
SH
Department of Life Science, Kwangju Institute of Science and Technology, Gwangju 500-712, South Korea.
Im
Young Jun
YJ
Rho
Seong Hwan
SH
Lee
Jun Hyuck
JH
Yang
Soyoung
S
Kim
Eunjoon
E
Eom
Soo Hyun
SH
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D051958:Nuclear Receptor Coactivator 2; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D051381:Rats; D014157:Transcription Factors / Q000737:chemistry*
C498003:Ncoa2 protein, rat; D051958:Nuclear Receptor Coactivator 2; D014157:Transcription Factors