Polarization oxygen sensor: a template for a class of fluorescence-based sensors.
We describe a novel oxygen polarization sensor based on a single molecule that changes the observed anisotropy of its emission with variation of the oxygen concentration. The approach is demonstrated both in solution and in solid films. A simple mathematical description of the sensor is included and experimentally verified. The technique demonstrated here creates opportunity for development of a new class of polarization-based dyes and sensors.
Kostov
Yordan
Y
Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County, Baltimore 21250, USA.
Gryczynski
Zygmunt
Z
Rao
Govind
G
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D002138:Calibration; D005454:Fluorescence Polarization / Q000295:instrumentation; D010100:Oxygen / Q000032:analysis*
D010100:Oxygen