Effects of chemical and physical agents on viability and infectivity of Encephalitozoon intestinalis determined by cell culture and flow cytometry.
We combined tissue culture and flow cytometry to assess the activities of various temperatures, chemicals, and disinfectants on the viability and infectivity of spores of Encephalitozoon intestinalis. Surfanios and benzalkonium chloride, disinfectants currently used in the hospital, were remarkably efficient in destroying spore viability and infectivity.
Santillana-Hayat
Maud
M
Parasitology-Mycology Laboratory, Department of Infectious Diseases, Saint-Louis Hospital, AP-HP and EA 3520, University of Paris 7, France.
Sarfati
Claudine
C
Fournier
Sandra
S
Chau
Françoise
F
Porcher
Raphaël
R
Molina
Jean-Michel
JM
Derouin
Francis
F
D000818:Animals; D001548:Benzalkonium Compounds / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004202:Disinfectants / Q000494:pharmacology*; D016819:Encephalitozoon / Q000187:drug effects* / Q000472:pathogenicity; D005434:Flow Cytometry; D013170:Spores; D013696:Temperature
D001548:Benzalkonium Compounds; D004202:Disinfectants