In vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium.
Chicken egg yolk antibody (IgY) raised against Salmonella enteritidis or Salmonella typhimurium was found in highly specific activity levels by ELISA. S. enteritidis- and S. typhimurium-specific IgY powder, prepared by freeze-drying the egg yolk water-soluble fraction, contained 15.5 and 10.0% of specific IgY, respectively. Anti-S. enteritidis IgY cross-reacted 55.3% with S. typhimurium. The cross-reactivity of anti-S. typhimurium IgY with S. enteritidis was 42.4%. Salmonella-specific IgY was demonstrated to inhibit Salmonella growth in liquid medium. The growth rate of S. enteritidis incubated with S. enteritidis-specific IgY was fourfold less than that of the control group during a 4-to-6-h incubation. Cell counts of S. typhimurium incubated with S. typhimurium-specific IgY were reduced by 1.6 log cfu/mL in comparison to that of the control group after 6 h of incubation. The specific binding activity of IgY was further evaluated by using immunofluorescence and immunoelectron microscopy. It was found that Salmonella-specific IgY could bind to the antigens expressed on the Salmonella surface, resulting in structural alterations of the bacterial surface.
Lee
E N
EN
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
Sunwoo
H H
HH
Menninen
K
K
Sim
J S
JS
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D000918:Antibody Specificity; D002645:Chickens; D015169:Colony Count, Microbial; D003429:Cross Reactions; D004530:Egg Yolk / Q000276:immunology* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007136:Immunoglobulins / Q000032:analysis / Q000276:immunology*; D016253:Microscopy, Immunoelectron / Q000662:veterinary; D011208:Powders; D012477:Salmonella enteritidis / Q000254:growth & development / Q000276:immunology*; D012486:Salmonella typhimurium / Q000254:growth & development / Q000276:immunology*; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; C016512:IgY; D007136:Immunoglobulins; D011208:Powders