[Isolation of novel expression sequences of C2H2 type zinc finger protein gene from human brain tissue according to the conservation of zinc finger motif].
By low stringency PCR amplification of genomic DNA using the primers designed based on the conservation of zinc finger motif, we got 8 gradient eletrophoretic bands. After recovery of the second and third bands, the DNA fragments in them were cloned and sequenced. Compared to the GenBank database, among these 60 segments containing zinc finger motif, 23 segments were novel zinc finger genes' genomic segments. Then the human brain tissue cDNA library was screened, using these segments as probes, and 44 positive clones were obtained. Rescreening 28 of them, we got 20 rescreened clones. All of them were sequenced and sent to the GenBank DNA database for sequence analysis, the results showed that 16 were novel C2H2 type zinc finger protein cDNA segments. The cDNA segments encoding the novel C2H2 type zinc finger proteins provide the basic materials for cloning of full length cDNA of valuable novel zinc finger protein genes.
Sun
X Y
XY
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433.
Yu
L
L
Wu
G J
GJ
Fan
Y X
YX
Zheng
Q P
QP
Hu
P R
PR
Zhang
M
M
Jiang
Y
Y
Liu
S
S
Xu
Y F
YF
Zhao
S Y
SY
D000595:Amino Acid Sequence; D001923:Brain Chemistry / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016335:Zinc Fingers / Q000235:genetics*