Expression of the iorAB genes from Brevundimonas diminuta 7 encoding the molybdenum hydroxylase isoquinoline 1-oxidoreductase in Pseudomonas putida.
Isoquinoline 1-oxidoreductase (Ior) from Brevundimonas diminuta 7, encoded by iorAB, is a molybdenum hydroxylase containing a molybdopterin cytosine dinucleotide molybdenum cofactor (Mo-MCD) and two distinct [2Fe2S] clusters. The iorAB genes were inserted into pJB653, generating pIL1. Pseudomonas putida KT2440, and P. putida 86 which produces a Mo-MCD-containing quinoline 2-oxidoreductase when grown on quinoline, were used as recipients for pIL1. Upon induction of gene expression, both clones produced Ior protein, but Ior activity was not detectable in P. putida KT2440 pIL1. In P. putida 86 pIL1, formation of catalytically active Ior required the presence of quinoline, suggesting that accessory gene(s) encoding product(s) essential for the assembly of catalytically competent Ior is (are) part of the quinoline regulon in P. putida 86.
Israel
Ilka
I
AG Mikrobiologie, FB 7, Carl von Ossietzky Universität Oldenburg, P.O. Box 2503, Germany.
Sohni
Monika
M
Fetzner
Susanne
S
D001483:Base Sequence; D016934:Caulobacter / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D005798:Genes, Bacterial; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism; D009876:Operon; D044925:Oxidoreductases Acting on CH-CH Group Donors; D016958:Pseudomonas putida / Q000235:genetics*; D011804:Quinolines / Q000378:metabolism
D017931:DNA Primers; D011804:Quinolines; C037219:quinoline; D006899:Mixed Function Oxygenases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C086842:isoquinoline 1-oxidoreductase