Gastric H-K-ATPase and acid-resistant surface proteins.
Despite the fact that mucus and bicarbonate are important macroscopic components of the gastric mucosal barrier, severe acidic and peptic conditions surely exist at the apical membrane of gastric glandular cells, and these membranes must have highly specialized adaptations to oppose external insults. Parietal cells abundantly express the heterodimeric, acid-pumping H-K-ATPase in their apical membranes. Its beta-subunit (HKbeta), a glycoprotein with >70% of its mass and all its oligosaccharides on the extracellular side, may play a protective role. Here, we show that the extracellular domain of HKbeta is highly resistant to trypsin in the native state (much more than that of the structurally related Na-K-ATPase beta-subunit) and requires denaturation to expose tryptic sites. Native HKbeta also resists other proteases, such as chymotrypsin and V8 protease, which hydrolyze at hydrophobic and anionic amino acids, respectively. Removal of terminal alpha-anomeric-linked galactose does not appreciably alter tryptic sensitivity of HKbeta. However, full deglycosylation makes HKbeta much more susceptible to all proteases tested, including pepsin at pH <2.0. We propose that 1) intrinsic folding of HKbeta, 2) bonding forces between subunits, and 3) oligosaccharides on HKbeta provide a luminal protein domain that resists gastric lytic conditions. Protein folding that protects susceptible charged amino acids and is maintained by disulfide bonding and hydrophilic oligosaccharides would provide a stable structure in the face of large pH changes. The H-K-ATPase is an obvious model, but other gastric luminally exposed proteins are likely to possess analogous protective specializations.
Thangarajah
Hariharan
H
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3200, USA.
Wong
Aline
A
Chow
Dar C
DC
Crothers
James M
JM
Forte
John G
JG
D000818:Animals; D002918:Chymotrypsin / Q000378:metabolism / Q000494:pharmacology; D004220:Disulfides / Q000378:metabolism; D005110:Extracellular Space / Q000201:enzymology; D005690:Galactose / Q000378:metabolism; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000201:enzymology*; D006023:Glycoproteins / Q000378:metabolism*; D017506:H(+)-K(+)-Exchanging ATPase / Q000737:chemistry* / Q000378:metabolism*; D020035:Holoenzymes / Q000378:metabolism; D011489:Protein Denaturation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011817:Rabbits; D012697:Serine Endopeptidases / Q000378:metabolism / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism / Q000494:pharmacology
D004220:Disulfides; D006023:Glycoproteins; D020035:Holoenzymes; D012697:Serine Endopeptidases; D002918:Chymotrypsin; C028344:glutamyl endopeptidase; D014357:Trypsin; D017506:H(+)-K(+)-Exchanging ATPase; D005690:Galactose