title: First identification of a ranavirus from green pythons (Chondropython viridis).
abstract: Ten juvenile green pythons (Chondropython viridis) died or were euthanized shortly after having been illegally imported into Australia from Indonesia in 1998. Histologic examination of two of the three snakes that died revealed moderately severe chronic ulceration of the nasal mucosa and focal or periacinar degeneration and necrosis of the liver. In addition there was severe necrotizing inflammation of the pharyngeal submucosa accompanied by numerous macrophages, heterophils, and edema. An iridovirus was isolated in culture from several tissues and characterized by immunohistochemistry, electron microscopy, enzyme-linked immunosorbent Assay, polyacrylamide gel electrophoresis, polymerase chain reaction and sequence analysis, restriction endonuclease digestion, and DNA hybridization. This is the first report of a systemic ranavirus infection in any species of snake and is a new member of the genus, Ranavirus.
authors.affiliation: CSIRO, Division of Animal Health, Australian Animal Health Laboratory (AAHL), P.O. Bag 24, Geelong, 3220, Australia.
authors.forename: A D
authors.initials: AD
authors.lastname: Hyatt
authors.forename: M
authors.initials: M
authors.lastname: Williamson
authors.forename: B E H
authors.initials: BE
authors.lastname: Coupar
authors.forename: D
authors.initials: D
authors.lastname: Middleton
authors.forename: S G
authors.initials: SG
authors.lastname: Hengstberger
authors.forename: A R
authors.initials: AR
authors.lastname: Gould
authors.forename: P
authors.initials: P
authors.lastname: Selleck
authors.forename: T G
authors.initials: TG
authors.lastname: Wise
authors.forename: J
authors.initials: J
authors.lastname: Kattenbelt
authors.forename: A A
authors.initials: AA
authors.lastname: Cunningham
authors.forename: J
authors.initials: J
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017821:Boidae / Q000821:virology*; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D007214:Indonesia; D008099:Liver / Q000473:pathology; D008854:Microscopy, Electron / Q000662:veterinary; D008969:Molecular Sequence Data; D009297:Nasal Mucosa / Q000473:pathology; D010614:Pharynx / Q000473:pathology; D010802:Phylogeny; D011793:Queensland; D012327:RNA Virus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D017995:Ranavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D015183:Restriction Mapping / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary
chemical_list: D004279:DNA, Viral