Simultaneous detection of porcine circovirus 2 and porcine parvovirus in naturally and experimentally coinfected pigs by double in situ hybridization.
A technique for double in situ hybridization to simultaneously detect porcine circovirus 2 (PCV2) and porcine parvovirus (PPV) in the same tissue section was developed and applied to lymph node and spleen from 8 pigs experimentally coinfected with PCV2 and PPV and 20 pigs with naturally occurring postweaning multisystemic wasting syndrome. For double labeling studies, the tissue samples were processed sequentially, first for PPV in situ hybridization using a digoxigenin-labeled probe and then for PCV2 in situ hybridization using a biotinylated probe. Positive cells contained reaction products for PCV2 and PPV, respectively. Both PCV2 DNA and PPV DNA were observed mainly in the cytoplasm but occasionally in the nucleus. With double in situ hybridization, both PCV2 DNA and PPV DNA were simultaneously detected in lymph node and spleen. This double labeling technique for the detection of PCV2 and PPV is suitable both for pathogenesis studies and for diagnostic applications.
Kim
Junghyun
J
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon, Kyounggi Do, Republic of Korea.
Chae
Chanhee
C
D000818:Animals; D018173:Circoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D017925:Circovirus / Q000235:genetics* / Q000472:pathogenicity; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D017403:In Situ Hybridization / Q000379:methods / Q000662:veterinary*; D008198:Lymph Nodes / Q000821:virology; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D028323:Parvovirus, Porcine / Q000235:genetics* / Q000472:pathogenicity; D013154:Spleen / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000235:genetics / Q000821:virology
D017931:DNA Primers; D004279:DNA, Viral