Genetic diversity in european and Argentinian cultivated potatoes (Solanum tuberosum subsp. tuberosum) detected by inter-simple sequence repeats (ISSRs).
In this study, the use of inter-simple sequence repeats (ISSR) to assess genetic diversity between cultivated potatoes (Solanum tuberosum subsp. tuberosum) is reported. ISSR technology rapidly reveals high polymorphic fingerprints and thus determines the genetic diversity among potato cultivars. Nine primers were selected according to the number of amplified markers and the level of polymorphism detected. Three primers (GAG(CAA)5, CTG(AG)8, and (AG)8) were used to cluster the 28 potato accessions and 77 polymorphic markers were sufficient to identify all of the accessions. Among the 15 simple sequence repeat (SSR) motifs tested, the most abundant were CAA and AG. Argentinian- and European-grown potatoes were easily distinguished, with a higher level of genetic diversity among potatoes from Argentina. An ISSR study using a limited number of cultivars and very few primers clearly differentiated between all cultivars, thus ISSR was revealed to be a good tool for the genetic identification of potato and for future germplasm-management programs.
Bornet
B
B
Laboratoire de Biotechnologie et Physiologie Végétales, ISAMOR, Université de Bretagne Occidentale, Plouzané, France. benjamin.bornet@univ-brest.fr
Goraguer
F
F
Joly
G
G
Branchard
M
M
D001118:Argentina; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers; D005060:Europe; D005819:Genetic Markers; D014644:Genetic Variation; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D011198:Solanum tuberosum / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers