Molecular characterization of the vanE gene cluster in vancomycin-resistant Enterococcus faecalis N00-410 isolated in Canada.
The vanE operon was characterized from Enterococcus faecalis N00-410 (MIC of vancomycin = 24 microg/ml). The organization of the vanE operon was identical to that of the vanC1 operon from Enterococcus gallinarum, with protein identities ranging from 46 to 63%. An open reading frame located downstream of the vanE operon showed significant homology to a number of integrase genes, all of which are located downstream of the chromosomal GMP synthase gene guaA.
Boyd
D A
DA
Nosocomial Infections, National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba, Canada.
Cabral
T
T
Van Caeseele
P
P
Wylie
J
J
Mulvey
M R
MR
D002170:Canada; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D005810:Multigene Family / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D020713:Vancomycin Resistance / Q000235:genetics*