The sctR of Salmonella enterica serova Typhimurium encoding a homologue of MerR protein is involved in the copper-responsive regulation of cuiD.
We have identified the cuiD gene in Salmonella enterica serova Typhimurium that codes for a putative multicopper oxidase. Expression of cuiD is induced by copper ion and its promoter/operator has sequence similarity to the promoters controlled by the transcriptional regulators of the MerR family. We also identified and isolated a gene from S. enterica serova Typhimurium that encodes a 138-amino acid residue protein, sctR, a new member of the MerR family of transcriptional regulators. Transposon-insertional disruption of sctR shows sensitivity to copper ion and no response of cuiD expression. Copper-responsive induction and copper tolerance were restored by providing sctR in trans, suggesting that SctR plays an important role in intracellular copper detoxification.
Kim
Jin Sook
JS
Department of Microbiology, HanNam University, 133 Ojung-Dong, Taeduk-Ku, Taejon 306-791, South Korea.
Kim
Mi Hwa
MH
Joe
Min Ho
MH
Song
Sang Sun
SS
Lee
In Soo
IS
Choi
Soon-Yong
SY
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003300:Copper / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009876:Operon; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001616:beta-Galactosidase / Q000187:drug effects / Q000235:genetics
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C051124:MerR protein, Bacteria; C460088:SctR protein, Salmonella typhimurium; D014157:Transcription Factors; D003300:Copper; C460089:CiuD protein, Salmonella typhimurium; D010088:Oxidoreductases; C024748:copper oxidase; D001616:beta-Galactosidase