Studies on the development of DNA vaccine against Cysticercus cellulosae infection and its efficacy.
DNA vaccine against Cysticercus cellulosae infection was developed and its efficacy was tested. A pair of primers specific to antigen B gene of C. cellulosae was designed which amplified the gene successfully with RT-PCR. The gene was ligated to PV93 vector, and the recombinant of antigen B gene and PV93 was transformed to JM83 cells. The transformed JM83 cells were cultured in a large scale and the plasmid purified. Based on the recombinant plasmid. a DNA vaccine was developed and used to vaccinate two groups of experimental pigs. In each group, there was a routine vaccine, an enhanced vaccine and a control group. Groups 1 and 2 were challenged at 4 months and at 14 days post vaccination respectively with eggs of Taenia solium. The antibody response was also tested with ELISA. The results suggested that all animals vaccinated AgB gene DNA vaccine, no matter by routine or enhanced vaccine, their antibodies reached maximum peak 23 days post vaccination and decreased gradually. When the animals were challenged 4 months after vaccination, they had strong immunity and the parasites decrease rates were 91.2% and 93.1% respectively. When pigs vaccinated with AgB gene DNA vaccine were challenged 14 days post vaccination with 18,000 eggs/pig. The animals showed strong immunity and the parasite decrease rates were 99.5% and 84.9% respectively. However at that time, the antibodies did not reach the peak. While in the control group, the number of C. cellulosae was as many as 2,500. It was concluded that the pigs vaccinated with DNA vaccine had strong immunity against infection of eggs of T. solium.
Cai
X
X
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Gansu, People's Republic of China. caixp@public.lz.gs.cn
Chai
Z
Z
Jing
Z
Z
Wang
P
P
Luo
X
X
Chen
J
J
Dou
Y
Y
Feng
S
S
Su
C
C
Jin
J
J
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D003551:Cysticercosis / Q000517:prevention & control / Q000662:veterinary*; D003552:Cysticercus / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D008460:Meat / Q000469:parasitology; D018510:RNA, Helminth / Q000737:chemistry / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology / Q000517:prevention & control*; D016896:Treatment Outcome; D014611:Vaccination / Q000592:standards / Q000662:veterinary; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology / Q000592:standards*; D015047:Zoonoses
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018510:RNA, Helminth; D019444:Vaccines, DNA