[Effect of 8-(N,N'-diethylamino)-n-octyl-3,4,5-trimethoxybenzoate on [Ca2+]i and the release of NO in cultured endothelial cells of the calf middle cerebral artery].
The effect of 8-(N,N'-diethylamino)-n-octyl-3,4,5-trimethoxybenzoate(TMB-8) on [Ca2+]i and the release of NO in cultured endothelial cells of the calf middle cerebral artery was studied by a system of measurement of AR-CM-MIC, using Fura-2/AM as a fluorescent indicator and the oxy-haemoglobin method. In the presence of extracellular Ca2+ 1.3 mmol.L-1, the resting [Ca2+]i and the extinction difference(delta E) of between 401 and 410 nm were not changed by TMB-8 12.5 and 25 mumol.L-1, but were increased by TMB-8 50 and 100 mumol.L-1. The rise of delta E induced by TMB-8 50 and 100 mumol.L-1 was blocked completely by L-NAME. The elevation of [Ca2+]i was related to the release of NO. The result suggested that TMB-8 increased resting [Ca2+]i of endothelial cells of the calf middle cerebral artery and induced NO release.
Wang
B
B
Department of Pharmacology, Nanjing Medical University, Nanjing 210029.
Yu
H
H
Xiao
J
J
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D008297:Male; D020768:Middle Cerebral Artery / Q000166:cytology; D009569:Nitric Oxide / Q000378:metabolism*; D014665:Vasodilator Agents / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D014665:Vasodilator Agents; D009569:Nitric Oxide; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; D002118:Calcium