Stage-specific expression of Smad2 and Smad3 during folliculogenesis.
Paracrine and autocrine growth factors can affect many different aspects of ovarian follicle development. Many members of the transforming growth factor beta (TGFbeta) family of growth factors and their receptors are expressed in developing follicles. However, the presence and function of the family of the TGFbeta signaling molecules known as Smads have not been evaluated during follicle development. We have demonstrated that two Smad family members that function as mediators for both activin and TGFbeta are expressed in granulosa cells of preantral follicles but not in large antral follicles. Smad2 expression, but not Smad3 expression, returns in luteal cells. Both Smad2 and Smad3 are translocated to the nucleus of granulosa cells in response to treatment with either TGFbeta or activin. However, Smad2 is more responsive to activin stimulation, and Smad3 is more responsive to TGFbeta stimulation. Stage-specific expression and differing ligand sensitivity of signaling molecules may work together to allow different effects of TGFbeta family ligands using the same signaling pathways over the course of follicular development.
Xu
Jian
J
Magee-Womens Research Institute and the Department of Obstetrics and Gynecology and Reproductive Biology of the University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA.
Oakley
Jennifer
J
McGee
Elizabeth A
EA
D028341:Activins / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003338:Corpus Luteum / Q000737:chemistry; D030762:Estrous Cycle; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D015870:Gene Expression; D006064:Gonadotropins, Equine / Q000494:pharmacology; D006107:Granulosa Cells / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D028322:Inhibin-beta Subunits / Q000494:pharmacology; D009865:Oocytes / Q000737:chemistry; D006080:Ovarian Follicle / Q000737:chemistry / Q000502:physiology*; D010053:Ovary / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013799:Theca Cells / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D016212:Transforming Growth Factor beta; C422405:activin A; D028341:Activins; D005640:Follicle Stimulating Hormone; D028322:Inhibin-beta Subunits