title: Oviedomycin, an unusual angucyclinone encoded by genes of the oleandomycin-producer Streptomyces antibioticus ATCC11891.
abstract: Our investigations on the discovery of novel natural metabolites using type II polyketide synthase gene probes (actI/III) yielded an unusual angucyclinone, oviedomycin (2), when applied to the oleandomycin (1) producer Streptomyces antibioticus ATCC11891. The novel natural product was produced using S. albus R(-)M(-) as a host strain, into which a cosmid containing the oviedomycin gene cluster was transformed. Its structure was elucidated by NMR spectroscopy and mass spectrometry.
authors.affiliation: Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, 33006 Oviedo, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Méndez
authors.forename: Eva
authors.initials: E
authors.lastname: Künzel
authors.forename: Fredilyn
authors.initials: F
authors.lastname: Lipata
authors.forename: Felipe
authors.initials: F
authors.lastname: Lombó
authors.forename: William
authors.initials: W
authors.lastname: Cotham
authors.forename: Michael
authors.initials: M
authors.lastname: Walla
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Bearden
authors.forename: Alfredo F
authors.initials: AF
authors.lastname: Braña
authors.forename: Jose A
authors.initials: JA
authors.lastname: Salas
authors.forename: Jürgen
authors.initials: J
authors.lastname: Rohr
mesh_terms: D000617:Aminoglycosides / Q000737:chemistry / Q000302:isolation & purification*; D004988:Ethers, Cyclic / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013303:Streptomyces antibioticus / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000617:Aminoglycosides; D004988:Ethers, Cyclic; D009097:Multienzyme Complexes; C461780:oviedomycin