[Detection of heroin metabolites: 6-monoacetylmorphine and morphine in human hair by GC/MS].
This paper presents a method to detect the main metabolites of heroin: 6-monoacetylmorphine (6-MAM) and morphine (MOR) in human hair using GC/MS-SIM. The hair specimens were washed with special solvents and cut into about 0.5 mm pieces. Ethylmorphine was added as internal standard and HCl solution for hydrolysis. After hydrolysis, 6-MAM and MOR were extracted by a mixture of solvents (chloroform-isopropyl alcohol-heptane 50:17:33). The residue of the extract was derivatized with N-methyl-N-trimethylsilyl trifluoroacetamide(MSTFA), then the trimethylsilyl(TMS)-derivatives were qualitatively and quantitatively analyzed using GC/MS-SIM. The correlation coefficients for 6-MAM and MOR were 0.9996 and 0.9997, respectively. The recoveries of both 6-MAM and MOR were over 50%. The RSD of within-day and between-day was less than 8% and 10%, respectively. The lower limit of detection of both 6-MAM and MOR was 0.5 ng.mg-1. Hair samples of 12 drug abusers were analyzed using this method, 8 of them gave positive results. This method is simple, accurate and sensitive. It is very suitable for routine case work.
Hong
Z
Z
College of Parmacy, Second Military Medical University, Shanghai 200433.
Wu
Y
Y
Wu
M
M
Zhuo
X
X
D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry*; D003932:Heroin / Q000378:metabolism*; D006556:Heroin Dependence / Q000378:metabolism; D006801:Humans; D009020:Morphine / Q000032:analysis*; D009022:Morphine Derivatives / Q000032:analysis*
D009022:Morphine Derivatives; D003932:Heroin; D009020:Morphine; C026979:6-O-monoacetylmorphine