[Low-molecular-weight chromium-binding substances in hepatic and nephric tissues of chromium-exposed rats].
Male Wistar rats were injected intraperitoneally with potassium dichromate(2.55 mg Cr6+ per kg body weight) for 7 days, and were observed continuously for 7 days after stopping Cr injection. The results were that concentrations of chromium-binding substances (HMW-Cr, LMW-Cr, and MMW-Cr) increased significantly with the extension of Cr injection time; MMW-Cr was not found in the control group. MMW-Cr may be a valuable indicator in diagnosing chromium toxication. It was efficient to use Sephadex G-50 column chromatography and ultra-violet absorption spectrometry-atomic absorption spectrophotometry in isolation, and then it was followed by quantitative analysis of chromium-binding substances.
Gao
Z
Z
Environmental Medicine Research Laboratory, Hunan Medical University, Changsha 410078.
Zeng
M
M
Wang
X
X
An
F
F
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D011192:Potassium Dichromate / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C485790:chromodulin protein, rat; D011192:Potassium Dichromate