Maternal lineages of Pinus densata, a diploid hybrid.
Previous morphological, allozyme and chloroplast DNA data have suggested that Pinus densata originated through hybridization between P. tabuliformis and P. yunnanensis. In the present study, sequence and restriction site analyses of maternally inherited mitochondrial nad1 intron were used to detect variation patterns in 19 populations of P. tabuliformis, P. yunnanensis and P. densata. A total of three mitotypes (A, B, C) were detected. All but one of the populations of P. yunnanensis possessed mitotype B while all populations of P. tabuliformis had mitotype A. Pinus densata populations, on the other hand, harboured both mitotypes A and B, which are characteristic of P. tabuliformis and P. yunnanensis, respectively. This result gives strong additional evidence supporting the hybrid origin of this diploid pine. The distribution of mitotypes indicated very different mating compositions and evolutionary history among P. densata populations. It seems that local founder populations and backcrosses may have played important roles in the early establishment of P. densata populations. The uplift of the Tibetan Plateau had a significant impact on the distribution of maternal lineages of P. densata populations.
Song
Bao-Hua
BH
Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Wang
Xiao-Quan
XQ
Wang
Xiao-Ru
XR
Sun
Lan-Ju
LJ
Hong
De-Yuan
DY
Peng
Pei-Hao
PH
D002681:China; D018742:DNA, Chloroplast / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics; D014644:Genetic Variation; D006824:Hybridization, Genetic; D007438:Introns; D024101:Mitochondrial Proteins / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D028223:Pinus / Q000235:genetics*; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length
D018742:DNA, Chloroplast; D004272:DNA, Mitochondrial; D024101:Mitochondrial Proteins; D010940:Plant Proteins