Comparison between the counter immunoelectrophoresis test and mouse neutralization test for the detection of antibodies against rabies virus in dog sera.
The detection of rabies antibodies is extremely valuable for epidemiological studies, determination of immune status in man, animals, and for the diagnosis of the disease. Several serological procedures have been described for this purpose. The present study reports a comparison between counterimmunoelectrophoresis test (CIET) and mouse neutralization test (MNT) in the detection of antibodies against rabies virus from 212 serum samples of vaccinated dogs. The agreement between both techniques was 79.7% and a significative association was demonstrated. The correlation coefficients between MNT and the CIET titers was determined considering 88 samples showing positive results in both techniques [CIET = 2 and MNT = 5 (0.13 IU/ml)] and resulted r2 = 0.7926 (p < 0.001). The performance of CIET system was technically simple, cheap and rapid, and thereby it could be useful for serological monitoring of dog vaccination campaigns as well as for individual analysis.
Silva
Luzia Helena Queiroz da
LH
Departamento de Apoio, Produção e Saúde Animal, Curso de Medicina Veterinária, Universidade Estadual Paulista, Araçatuba, SP, 16050-680, Brasil. lhqsilva@fmva.unesp.br
Bissoto
César Eduardo
CE
Carvalho
Cristiano de
Cd
Cardoso
Tereza Cristina
TC
Pinheiro
Devani M
DM
Perri
Silvia Helena Venturoli
SH
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D003378:Counterimmunoelectrophoresis; D004283:Dog Diseases / Q000175:diagnosis / Q000276:immunology / Q000517:prevention & control; D004285:Dogs; D016014:Linear Models; D051379:Mice; D009500:Neutralization Tests; D011818:Rabies / Q000175:diagnosis / Q000276:immunology / Q000517:prevention & control; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000276:immunology*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D011819:Rabies Vaccines