title: In situ detection of cellular and abnormal isoforms of prion protein in brains of cattle with bovine spongiform encephalopathy and sheep with scrapie by use of a histoblot technique.
abstract: To detect prion protein, brains from 5 cattle naturally affected with bovine spongiform encephalopathy (BSE) and 3 sheep naturally affected with scrapie were examined and compared with brains of normal cattle and sheep using a histoblot technique. The technique enabled the in situ distinctive detection of the cellular (PrP(C)) and abnormal (PrP(Sc)) isoforms of the prion protein. In BSE- or scrapie-affected brains, the Prp(C) signal decreased, especially in those areas where the PrP(Sc) signal was detected.
authors.affiliation: National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors.forename: Kumiko M
authors.initials: KM
authors.lastname: Kimura
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Makoto
authors.initials: M
authors.lastname: Haritani
authors.forename: Minoru
authors.initials: M
authors.lastname: Narita
authors.forename: Peter
authors.initials: P
authors.lastname: Belleby
authors.forename: Julie
authors.initials: J
authors.lastname: Smith
authors.forename: Yvonne I
authors.initials: YI
authors.lastname: Spencer
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis* / Q000473:pathology; D007150:Immunohistochemistry / Q000379:methods*; D011328:Prions / Q000032:analysis*; D020033:Protein Isoforms / Q000032:analysis; D012608:Scrapie / Q000175:diagnosis* / Q000473:pathology; D012680:Sensitivity and Specificity; D012756:Sheep
chemical_list: D011328:Prions; D020033:Protein Isoforms