[Growth-coupled lipid synthesis in Mortierella alpina LPM 301, a producer of arachidonic acid].
Mortierella alpina LPM 301, a producer of arachidonic acid (ARA), was found to possess a unique property of intense lipid synthesis in the period of active mycelium growth. Under batch cultivation of this strain in glucose-containing media with potassium nitrate or urea, the bulk of lipids (28-35% of dry biomass) was produced at the end of the exponential growth phase and remained almost unaltered in the stationary phase. The ARA content of lipids comprised 42-50% at the beginning of the stationary phase and increased continuously after glucose depletion in the medium due to the turnover of intracellular fatty acids; by the end of fermentation (189-210 h), the amount of ARA reached 46-60% of the total fatty acids (16-19% of dry mycelium). Plausible regulatory mechanisms of the growth-coupled lipid synthesis in microorganisms are discussed.
Eroshin
V K
VK
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.
Dediukhina
E G
EG
Satrutdinov
A D
AD
Chistiakova
T I
TI
D016718:Arachidonic Acid / Q000032:analysis / Q000096:biosynthesis; D003470:Culture Media; D005947:Glucose; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis*; D020094:Mortierella / Q000254:growth & development / Q000378:metabolism*; D025282:Mycelium / Q000737:chemistry / Q000378:metabolism; D009566:Nitrates; D017680:Potassium Compounds; D014508:Urea
D003470:Culture Media; D008055:Lipids; D009566:Nitrates; D017680:Potassium Compounds; D016718:Arachidonic Acid; D014508:Urea; D005947:Glucose; C023844:potassium nitrate