Slug is a novel downstream target of MyoD. Temporal profiling in muscle regeneration.
Temporal expression profiling was utilized to define transcriptional regulatory pathways in vivo in a mouse muscle regeneration model. Potential downstream targets of MyoD were identified by temporal expression, promoter data base mining, and gel shift assays; Slug and calpain 6 were identified as novel MyoD targets. Slug, a member of the snail/slug family of zinc finger transcriptional repressors critical for mesoderm/ectoderm development, was further shown to be a downstream target by using promoter/reporter constructs and demonstration of defective muscle regeneration in Slug null mice.
Zhao
Po
P
Research Center for Genetic Medicine, Children's National Medical Center, and Genetics Program, George Washington University, Washington, D. C. 20010, USA.
Iezzi
Simona
S
Carver
Ethan
E
Dressman
Devin
D
Gridley
Thomas
T
Sartorelli
Vittorio
V
Hoffman
Eric P
EP
D000818:Animals; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology*; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012038:Regeneration / Q000235:genetics*; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D017570:MyoD Protein; C000604407:SNAI1 protein, human; C000604449:Snai2 protein, mouse; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors