title: Lentivirus lytic peptide 1 perturbs both outer and inner membranes of Serratia marcescens.
abstract: Bis-lentivirus lytic protein 1 (Bis-LLP1) and polymyxin B exhibited similar killing activities against Serratia marcescens. By electron microscopy, bis-LLP1 interacted with the outer and cytoplasmic bacterial membranes, while polymyxin B affected only the outer membrane. The results of standard biochemical probes supported the findings of the electron microscopy studies, suggesting that these antimicrobial peptides have different mechanisms of action.
authors.affiliation: Department of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA.
authors.forename: Shruti M
authors.initials: SM
authors.lastname: Phadke
authors.forename: Vanja
authors.initials: V
authors.lastname: Lazarevic
authors.forename: Caroline C
authors.initials: CC
authors.lastname: Bahr
authors.forename: Kazi
authors.initials: K
authors.lastname: Islam
authors.forename: Donna Beer
authors.initials: DB
authors.lastname: Stolz
authors.forename: Simon
authors.initials: S
authors.lastname: Watkins
authors.forename: Sarah B
authors.initials: SB
authors.lastname: Tencza
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Vogel
authors.forename: Ronald C
authors.initials: RC
authors.lastname: Montelaro
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Mietzner
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure; D005614:Freeze Fracturing; D008565:Membrane Proteins / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D011112:Polymyxin B / Q000494:pharmacology; D012706:Serratia marcescens / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents; D008565:Membrane Proteins; D011112:Polymyxin B