title: Somatic cell mutation in pediatric patients undergoing allogeneic bone marrow transplantation.
abstract: In order to examine whether bone marrow transplantation (BMT) has genotoxic effects in vivo, mutant frequencies (Mfs) at the hypoxanthine-guanine phosphoribosyl transferase (Hprt) locus were evaluated. Thirty-seven pediatric patients who had received allogeneic BMT for various hematologic or immunologic disorders were enrolled. Nine out of the 37 patients (24.3%) were found to have Hprt-Mfs exceeding the 99% confidence limits calculated from observation of healthy controls. Among factors including gender, primary disease of the patient, donor-recipient histocompatibility relationship, age of donor, and total body irradiation as conditioning regimen, none was associated with an increased Hprt-Mf. In three patients who had chimerism in their peripheral blood after BMT, Hprt mutant clones turned out to be of donor- or recipient-origin. Mfs at the T-cell receptor (TCR) locus were examined in 28 patients. Four patients (14.3%) were found to have increased TCR-Mfs. However, there were not any patients who showed elevation of both Hprt-and TCR-Mfs. These data, taken together, suggest that BMT may cause genotoxicity in vivo in some patients.
authors.affiliation: Department of Pediatrics, Kyoto University, Kyoto, Japan.
authors.forename: Keigo
authors.initials: K
authors.lastname: Hamahata
authors.forename: Masaru
authors.initials: M
authors.lastname: Kubota
authors.forename: Ikuya
authors.initials: I
authors.lastname: Usami
authors.forename: Ying-Wei
authors.initials: YW
authors.lastname: Lin
authors.forename: Ken
authors.initials: K
authors.lastname: Shimizu
authors.forename: Akira
authors.initials: A
authors.lastname: Morimoto
authors.forename: Tatsutoshi
authors.initials: T
authors.lastname: Nakahata
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D016026:Bone Marrow Transplantation; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007223:Infant; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D014184:Transplantation, Homologous
chemical_list: D011948:Receptors, Antigen, T-Cell; D007041:Hypoxanthine Phosphoribosyltransferase