Inflammatory responses and mucus secretion in rats with acute bronchiolitis induced by nickel chloride.
The effects of inhaled particulate matter in the workplace and outdoor environment on sensitive subpopulations are not sufficiently investigated in human and animal models. Thus, animal models for pulmonary diseases are necessary for appropriate risk assessment of toxic materials. We studied biochemical characteristics of an acute inflammatory process induced by inhalation of nickel chloride aerosols in rats. Acute bronchiolitis was induced by inhalation of nickel chloride aerosols for 5 days in Wistar rats according to the method described by Kyono et al. (1999). Deterioration and recovery from inflammatory responses were evaluated by analyzing markers of inflammation in bronchoalveolar lavage (BAL) fluid. Experimental animals were sacrificed during and after the nickel aerosol exposure period. The number of neutrophils markedly increased to approximately 0.5 x 10(3) cells/microl BAL fluid during nickel aerosol exposure, accompanied by increase of total protein, soluble L-selectin, cytokine-induced neutrophil chemoattractant/growth-regulated gene products (CINC/GRO), elastolytic activity, trypsin inhibitory capacity, beta-glucuronidase activity, fucose, and sialic acid in BAL fluid compared with those of the control group. There was correlation between number of leukocytes and soluble L-selectin concentration. The number of pulmonary macrophages in BAL fluid decreased to approximately 15% of those of the control group on the days of nickel aerosol exposure. The level of CINC/GRO recovered to that of the control group on day 3 after cessation of the nickel aerosol exposure. However, other inflammatory markers remained at the elevated levels. Changes in the markers of inflammation during and after the nickel aerosol exposure were consistent with previously reported morphological findings. The results indicated that this animal model is potentially useful as an acute bronchiolitis model.
Ishihara
Yoko
Y
Department of Hygiene and Public Health (I), School of Medicine, Tokyo Women's Medical University, Tokyo, Japan. ishihara@research.twmu.ac.jp
Kyono
Hiroko
H
Serita
Fumio
F
Toya
Tadao
T
Kawashima
Hiroto
H
Miyasaka
Masayuki
M
D000818:Animals; D001988:Bronchiolitis / Q000139:chemically induced* / Q000276:immunology / Q000503:physiopathology; D004195:Disease Models, Animal; D007249:Inflammation / Q000473:pathology; D019570:Inhalation Exposure; D008297:Male; D009093:Mucus / Q000378:metabolism; D009532:Nickel / Q000008:administration & dosage / Q000009:adverse effects*; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar
C022838:nickel chloride; D009532:Nickel