[Characterization of the cell-bound polysaccharides of Bifidobacterium adolescentis 94 BIM].
The cell-bound polysaccharides (PSs) of Bifidobacterium adolescentis 94 BIM were stripped from the cell surface with 2% sodium dodecyl sulfate (SDS), 1.5% Cetavlon, and 1% Triton X-100 and purified by precipitation with 5 volumes of ethanol. According to the extraction conditions used, the polysaccharide preparations were designated as PS-SDS-6 degrees C, PS-SDS-100 degrees C, PS-Cet, and PS-Trit. The gel-permeation chromatography of the first two preparations with the use of a Bio-Gel P-10 column and 1% acetic acid as the eluant yielded two peaks, F1 and F2, which contained carbohydrates and no phosphorus. All polysaccharides were primarily composed of glucose and galactose. The polysaccharides PS-Cet and PS-Trit were found to be branched and contain glucose residues at the terminal position, position 4, and position 6, and galactose residue at position 3. PS-SDS-6 degrees C has a glucose residue at position 4.
Novik
G I
GI
Institute of Microbiology, National Academy of Sciences of Belarus, ul. Kuprevicha 2, Minsk, 220141 Belarus.
Astapovich
N I
NI
Kubler
J
J
Gam'ian
A
A
D001644:Bifidobacterium / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D002850:Chromatography, Gel; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D011092:Polyethylene Glycols; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate
D002593:Cetrimonium Compounds; D011135:Polysaccharides, Bacterial; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D005947:Glucose; D005690:Galactose; D000077286:Cetrimonium