title: Antibody cross-reactivity of alphaherpesviruses as mirrored in naturally infected primates.
abstract: The extent of antibody cross-reactivity of pooled antisera from rhesus monkeys, baboons, African green monkeys, langurs, sooty mangabeys and humans to 6 alphaherpesviruses (herpes B virus, herpes papio 2, simian agent 8, langur herpes virus and herpes simplex 1 & 2) was examined by two types of enzyme linked immunosorbent assays, an antibody capture assay (tELISA) and an antigen capture assay (dELISA). Percent cross-reactivity was calculated for each serum by comparison of the homologous reaction (100%) to the reaction with heterologous viruses. Comparison of the immunological reactivity of the mangabey antiserum pool to the panel of alphaherpesviruses indicated that these antibodies were induced by a yet unidentified alphaherpesvirus. In general, monkey sera were more cross-reactive to monkey herpesviruses than to human herpesviruses.However, the extent of cross-reactivity of monkey sera to human herpesviruses was relatively lower than the cross-reactivity of human sera to monkey herpes-viruses. Because of this phenomenon of "one-way" cross-reactivity that was also observed within the group of simian herpesviruses, it was difficult to rank the immunologic distances between the viruses in absolute terms.
authors.affiliation: Viral Immunology Center, Department of Biology, Georgia State University, Atlanta, Georgia 30303, USA. biodkkx@langate.gsu.edu
authors.forename: D
authors.initials: D
authors.lastname: Katz
authors.forename: W
authors.initials: W
authors.lastname: Shi
authors.forename: P W
authors.initials: PW
authors.lastname: Krug
authors.forename: R
authors.initials: R
authors.lastname: Henkel
authors.forename: H
authors.initials: H
authors.lastname: McClure
authors.forename: J K
authors.initials: JK
authors.lastname: Hilliard
mesh_terms: D018138:Alphaherpesvirinae / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D016665:Cercocebus atys / Q000276:immunology; D002520:Cercopithecidae / Q000276:immunology; D002522:Chlorocebus aethiops / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D008253:Macaca mulatta / Q000276:immunology; D010215:Papio / Q000276:immunology; D011323:Primates / Q000276:immunology*; D013045:Species Specificity
chemical_list: D000914:Antibodies, Viral; D007106:Immune Sera