Drosophila Enhancer of zeste protein interacts with dSAP18.
The Drosophila Enhancer of zeste [E(z)] gene encodes a member of the Polycomb group of transcriptional repressors. Here we report evidence for direct physical interaction between E(Z) and dSAP18, which previously has been shown to interact with Drosophila GAGA factor and BICOID proteins. dSAP18 shares extensive sequence similarity with a human polypeptide originally identified as a subunit of the SIN3A-HDAC (switch-independent 3-histone deacetylase) co-repressor complex. Yeast two-hybrid and in vitro binding assays demonstrate direct E(Z)-dSAP18 interaction and show that dSAP18 is capable of interacting with itself. Co-immunoprecipitation experiments provide evidence for in vivo association of E(Z) and dSAP18. Gel filtration analysis of embryo nuclear extracts shows that dSAP18 is present in native protein complexes ranging from approximately 1100 to approximately 450 kDa in molecular mass. These studies provide support for a model in which dSAP18 contributes to the activities of multiple protein complexes, and potentially may mediate interactions between distinct proteins and/or protein complexes.
Wang
Liangjun
L
Department of Biological Sciences, Southern Methodist University, Fondren Science Building, Dallas, TX 75275-0376, USA.
Ding
Lei
L
Jones
Clark A
CA
Jones
Richard S
RS
D000818:Animals; D000071183:Autophagy-Related Proteins; D015153:Blotting, Western; D002352:Carrier Proteins; D002850:Chromatography, Gel; D002874:Chromosome Mapping; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D063151:Polycomb Repressive Complex 2; D011233:Precipitin Tests; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
C498649:ATG16 protein, S cerevisiae; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C407043:bicoid interacting protein 1, Drosophila; C083329:E(z) protein, Drosophila; D063151:Polycomb Repressive Complex 2