title: The HMG1 ta(i)le.
abstract: We have studied structural changes in DNA/protein complexes using the CD spectroscopy, upon the interaction of HMG1-domains with calf thymus DNA at different ionic strengths. HMG1 protein isolated from calf thymus and recombinant HMG1-(A+B) protein were used. Recombinant protein HMG1-(A+B) represents a rat HMG1 lacking C-terminal acidic tail. At low ionic strength (15 mM NaCl) we observed similar behavior of both proteins upon interaction with DNA. Despite this, at higher ionic strength (150 mM NaCl) their interaction with DNA leads to a completely different structure of the complexes. In the case of HMG1-(A+B)/DNA complexes we observed the appearance of DNA fractions possessing very high optical activity. This could be a result of formation of the highly-ordered DNA structures modulated by the interaction with HMG1-domains. Thus the comparison studies of HMG1 and HMG1-(A+B) interaction with DNA show that negatively charged C-terminal tail of HMG1 modulates interaction of the protein with DNA. The striking difference of the behaviour of these two systems allows us to explain the functional role of multiple HMG1 domains in some regulatory and architectural proteins.
authors.affiliation: Molecular Biophysics Department, Physical Faculty of St.-Petersburg State University, 1 Ulianovskaya st., Stary Petergof, St.-Petersburg, 198904, Russia.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Polyanichko
authors.forename: Elena V
authors.initials: EV
authors.lastname: Chikhirzhina
authors.forename: Alexei N
authors.initials: AN
authors.lastname: Skvortsov
authors.forename: Elena I
authors.initials: EI
authors.lastname: Kostyleva
authors.forename: Pierre
authors.initials: P
authors.lastname: Colson
authors.forename: Claude
authors.initials: C
authors.lastname: Houssier
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Vorob'ev
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D004278:DNA, Superhelical / Q000378:metabolism; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D012965:Sodium Chloride
chemical_list: D004278:DNA, Superhelical; D024243:HMGB1 Protein; D011994:Recombinant Proteins; D012965:Sodium Chloride