title: Wheat FKBP73 functions in vitro as a molecular chaperone independently of its peptidyl prolyl cis-trans isomerase activity.
abstract: Peptidyl-prolyl cis-trans isomerases (PPIases) catalyse protein folding by accelerating the slow step of cis-trans isomerisation of peptidyl-prolyl bonds. Wheat (Triticum aestivum L.) FKBP73 (wFKBP73) is a peptidyl-prolyl cis-trans isomerase belonging to the FK506-binding protein (FKBP) family. It comprises three FKBP12-like domains, tetratricopeptide repeats and a calmodulin-binding domain (CaMbd). In vitro studies indicated that wFKBP73 possesses PPIase activity, binds calmodulin and forms a heterocomplex with mammalian p23 and wheat Hsp90 in wheat-germ lysate. To further study the role of wFKBP73 we have analysed its chaperone properties. Using the thermal unfolding and aggregation of citrate synthase (CS) as a model system, we have shown that the plant wFKBP73 exhibits chaperone activity, being able to suppress CS aggregation independently of its PPIase activity. The wFKBP73 interacts transiently with non-native CS and slows down its inactivation kinetics, whereas the mammalian homologue, hFKBP52 binds tightly to CS and does not affect its rate of inactivation. Hence, the first plant FKBP shown to function as a molecular chaperone has a mode of action different from that of the mammalian FKBP52.
authors.affiliation: Dep[artment of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel. adina@post.tau.ac.il
authors.forename: Isaac
authors.initials: I
authors.lastname: Kurek
authors.forename: Franziska
authors.initials: F
authors.lastname: Pirkl
authors.forename: Elke
authors.initials: E
authors.lastname: Fischer
authors.forename: Johannes
authors.initials: J
authors.lastname: Buchner
authors.forename: Adina
authors.initials: A
authors.lastname: Breiman
mesh_terms: D002148:Calmodulin-Binding Proteins / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000737:chemistry / Q000378:metabolism; D004789:Enzyme Activation; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism*; D019696:Peptidylprolyl Isomerase / Q000378:metabolism*; D017510:Protein Folding; D022061:Tacrolimus Binding Protein 1A / Q000378:metabolism; D022021:Tacrolimus Binding Proteins / Q000378:metabolism; D013696:Temperature; D014908:Triticum / Q000378:metabolism*
chemical_list: D002148:Calmodulin-Binding Proteins; D018841:HSP90 Heat-Shock Proteins; D018832:Molecular Chaperones; D006639:Histidine; D002950:Citrate (si)-Synthase; D022061:Tacrolimus Binding Protein 1A; D022021:Tacrolimus Binding Proteins; C403485:tacrolimus binding protein 4; D019696:Peptidylprolyl Isomerase