Identification of bluetongue virus serotype 2 (Corsican strain) by reverse-transcriptase PCR reaction analysis of segment 2 of the genome.
In October 2000, bluetongue virus was detected on the French island of Corsica. The disease was also reported in Sardinia, Calabria, Sicily and on the Spanish islands of Majorca and Minorca. This paper describes the use of molecular techniques for a rapid identification and serotype determination of serotype 2 of the virus. The nucleotide sequences of segments 2 and 7 of the genome of the Corsican strain were determined and its phylogenetic relationships are described.
Zientara
S
S
Agence Française de Sécurité Sanitaire des Aliments-Alfort, France.
Sailleau
C
C
Dauphin
G
G
Roquier
C
C
Rémond
E M
EM
Lebreton
F
F
Hammoumi
S
S
Dubois
E
E
Agier
C
C
Merle
G
G
Bréard
E
E
D000818:Animals; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis*; D004196:Disease Outbreaks; D005060:Europe / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D012703:Serotyping; D012756:Sheep; D012757:Sheep Diseases / Q000235:genetics / Q000821:virology*
D004279:DNA, Viral