title: Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34+ cells with small- and large-scale Miltenyi cell sorting system.
abstract: The purging efficacy of the Miltenyi sorting system was evaluated by qualitative and TaqMan quantitative polymerase chain reaction (PCR) in myeloma patients, using immunoglobulin genes. After small-scale selection, qualitative PCR showed that in 6 of 12 leukaphereses myeloma cells were no longer detectable. Envisaging a possible clinical application, the leukaphereses from three patients underwent large-scale selection. Qualitative PCR showed that myeloma cells were still detectable. Quantitative PCR, performed in two patients, showed a tumour depletion of 1 and 2 logs respectively. Although numbers are small, the promising results obtained with small-scale selection were not reproduced in large-scale experiments.
authors.affiliation: Department of Haematology, Laboratory of Molecular Haematology, Istituto H.S. Raffaele, Milan, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Voena
authors.forename: Giovanni
authors.initials: G
authors.lastname: Locatelli
authors.forename: Claudia
authors.initials: C
authors.lastname: Castellino
authors.forename: Paola
authors.initials: P
authors.lastname: Omedè
authors.forename: Marco
authors.initials: M
authors.lastname: Ladetto
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Zappone
authors.forename: Raffaella
authors.initials: R
authors.lastname: Milani
authors.forename: Vittorio
authors.initials: V
authors.lastname: Perfetti
authors.forename: Mario
authors.initials: M
authors.lastname: Boccadoro
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pileri
authors.forename: Paolo
authors.initials: P
authors.lastname: Lusso
authors.forename: Chiara
authors.initials: C
authors.lastname: Villa
authors.forename: Mauro
authors.initials: M
authors.lastname: Malnati
authors.forename: Paolo
authors.initials: P
authors.lastname: Corradini
mesh_terms: D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis*; D016465:Bone Marrow Purging / Q000379:methods*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D018189:Immunomagnetic Separation; D007937:Leukapheresis; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000473:pathology* / Q000628:therapy; D018365:Neoplasm, Residual; D016133:Polymerase Chain Reaction
chemical_list: D018952:Antigens, CD34