title: Identification of a tolerant locus on Arabidopsis thaliana to hypervirulent beet curly top virus CFH strain.
abstract: The infection of hosts by the geminivirus depends on the interactions between host and viral factors for viral DNA replication, viral gene expression, and the movement of virus throughout the hosts. This work reports that a hypervirulent strain of Beet curly top virus (BCTV) is different in its ability to infect several ecotypes of Arabidopsis thaliana. Symptoms appeared on Arabidopsis ecotypes around 7 to 10 d after inoculation with BCTV-CFH. Symptoms were more severe in ecotype SKKU including severe leaf curling and development of severely deformed and stunted boting compared to Col-O as a lab standard ecotype. One ecotype Cen-O was asymptomatic to BCTV-CFH infection. Studies of viral DNA replication and virus movement in three excised organs of asymptomatic Cen-O demonstrated that BCTV-CFH could replicate viral DNA and move systemically in this ecotype, suggesting that tolerance was due to the blocks of interactions between host and viral factors on symptom development. This asymptomatic phenotype is similar to the mutation of leftward ORFs, especially ORF R2. Genetic analysis of this ecotype Cen-O indicated that tolerance is due to a single recessive locus.
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea.
authors.forename: Seong-Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Jinkyung
authors.initials: J
authors.lastname: Hur
authors.forename: Jongbum
authors.initials: J
authors.lastname: Park
authors.forename: Sangseob
authors.initials: S
authors.lastname: Lee
authors.forename: Taek-Kyun
authors.initials: TK
authors.lastname: Lee
authors.forename: Man
authors.initials: M
authors.lastname: Chang
authors.forename: Keith R
authors.initials: KR
authors.lastname: Davi
authors.forename: Jeongha
authors.initials: J
authors.lastname: Kim
authors.forename: Sukchan
authors.initials: S
authors.lastname: Lee
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000821:virology*; D018744:DNA, Plant; D004279:DNA, Viral / Q000378:metabolism; D017791:Geminiviridae / Q000472:pathogenicity / Q000502:physiology*; D017343:Genes, Plant; D016366:Open Reading Frames / Q000235:genetics
chemical_list: D018744:DNA, Plant; D004279:DNA, Viral