Determination of the triterpenoid, betulinic acid, in Doliocarpus schottianus by HPLC.
The isolation, detection and quantification of betulinic acid in Doliocarpus schottianus are described. The isolation from the plant extract was made by column chromatography and centrifugal TLC, and betulinic acid was characterized by spectrometric methods. The detection and quantification were made by HPLC using a C18 column eluted with acetonitrile: water and detected at 210 nm. The results showed that the metabolite accumulates in the bark of the plant, but very small concentrations were also found in the leaves and wood.
de Oliveira
Brás H
BH
UFPR, Departamento de Química, CP19081, 81531-970, Curitiba-PR, Brazil. bho@quimica.ufpr.br
Santos
Cid A M
CA
Espíndola
Ana Paula D M
AP
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D053978:Pentacyclic Triterpenes; D010944:Plants / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D000094062:Betulinic Acid
D053978:Pentacyclic Triterpenes; D014315:Triterpenes; D000094062:Betulinic Acid