Effects of phospholipase Cgamma on Polo-like kinase 1 expression in human glioma cells.
OBJECTIVE
The precise regulation of the mammalian PLK-1 gene, involved in progression of the G2/M transition, remains to be clarified. Phospholipase Cgamma, associated with cell proliferation and invasion in human gliomas, could be one of the important candidates for the modulation of PLK-1 expression. Therefore, we studied the correlation between PLCgamma activity and PLK-1 expression and determined cell size and proliferation after PLCgamma inhibition in two glioma cell lines and a primary culture of a glioblastoma.


METHODS
The glioma cells were investigated with highly specific chemical and antisense inhibition of PLCgamma. The effects of the inhibition were checked by means of morphometrical, semiquantitative immunohistochemical methods, and MTT-assays.


RESULTS
The chemical and antisense inhibition of PLCgamma resulted in decreased expression of PLK-1 and reduced cell density in glioma cell lines as well as in a primary culture of a glioblastoma. These findings were verified by MTT-assays.


CONCLUSIONS
The activity of PLCgamma seems to modulate the expression of PLK-1. In further experiments serum-depleted cultures, stimulated by different growth factors, should be used after inhibitor pretreatment to study the in vivo conditions.
Dietzmann
K
K
Institute of Neuropathology, Otto-von-Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany. Knut.Dietzmann@Medizin.Uni-Magdeburg.de
Kirches
E
E
Mawrin
C
C
D018797:Cell Cycle Proteins; D002455:Cell Division; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000235:genetics; D016376:Oligonucleotides, Antisense; D051966:Phospholipase C gamma; D011494:Protein Kinases / Q000096:biosynthesis* / Q000235:genetics; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors* / Q000235:genetics; D000097869:Polo-Like Kinase 1
D018797:Cell Cycle Proteins; D007527:Isoenzymes; D016376:Oligonucleotides, Antisense; D051966:Phospholipase C gamma; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D010738:Type C Phospholipases; D000097869:Polo-Like Kinase 1