Comparative uptake of polyamines by prostate and non-prostate cancer cell lines.
The Km and Vmax of [14C]-radiolabeled polyamines were determined for PC-3 and AT3B-1 cell lines. With PC-3 Km values are in the following order: ornithine> spermidine> spermine> putrescine, while with AT3B-1 it was spermidine> ornithine> spermine> putrescine. To determine which of these polyamines exhibit higher accumulation, the relative uptake of all the four amines was studied with prostate (PC-3, AT3B-1, LNCaP) and non-prostate (MCF-7, KLN-205, OVCAR) cell lines at 10 and 20 microM after 1 hour. Spermine and spermidine accumulated at higher levels in prostate (AT3B-1 and LNCaP) over non-prostate cell lines (p < 0.01). Putrescine accumulated more in PC-3 and LNCaP than the non-prostate cancer cells.
Srinath
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P
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada.
McQuarrie
S A
SA
Suresh
M R
MR
D000818:Animals; D001943:Breast Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics*; D006801:Humans; D008297:Male; D009952:Ornithine / Q000493:pharmacokinetics; D011073:Polyamines / Q000493:pharmacokinetics*; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism*; D011700:Putrescine / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013095:Spermidine / Q000493:pharmacokinetics; D013096:Spermine / Q000493:pharmacokinetics; D014407:Tumor Cells, Cultured / Q000000981:diagnostic imaging / Q000378:metabolism; D014565:Urogenital Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism
D002250:Carbon Radioisotopes; D011073:Polyamines; D013096:Spermine; D009952:Ornithine; D013095:Spermidine; D011700:Putrescine