Biosynthesis of vitamin B6 in Rhizobium: in vitro synthesis of pyridoxine from 1-deoxy-D-xylulose and 4-hydroxy-L-threonine.
Pyridoxine (vitamin B6) in Rhizobium is synthesized from 1-deoxy-D-xylulose and 4-hydroxy-L-threonine. To define the pathway enzymatically, we established an enzyme reaction system with a crude enzyme solution of R. meliloti IFO14782. The enzyme reaction system required NAD+, NADP+, and ATP as coenzymes, and differed from the E. coli enzyme reaction system comprising PdxA and PdxJ proteins, which requires only NAD+ for formation of pyridoxine 5'-phosphate from 1-deoxy-D-xylulose 5-phosphate and 4-(phosphohydroxy)-L-threonine.
Tazoe
Masaaki
M
Department of Applied Microbiology, Nippon Roche Research Center, Kamakura, Kanagawa, Japan. masaaki.tazoe@roche.com
Ichikawa
Keiko
K
Hoshino
Tatsuo
T
D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D011736:Pyridoxine / Q000096:biosynthesis*; D012231:Rhizobium / Q000378:metabolism*; D013912:Threonine / Q000031:analogs & derivatives* / Q000378:metabolism*; D025101:Vitamin B 6 / Q000096:biosynthesis*; D014996:Xylulose / Q000031:analogs & derivatives* / Q000378:metabolism*
D001426:Bacterial Proteins; D009243:NAD; C011285:hydroxythreonine; D013912:Threonine; D009249:NADP; D014996:Xylulose; C029724:1-deoxy-2-pentulose; D025101:Vitamin B 6; D000255:Adenosine Triphosphate; D011736:Pyridoxine