title: Small bowel resection in the rat: the effect upon bone and mineral metabolism.
abstract: OBJECTIVE
  We analyzed bone and mineral metabolism after long-segment small bowel resection in the rat to detect functional and morphological alterations and to determine the development of osteopathy.
  
  
  METHODS
  Twelve-week-old male Lewis rats were randomized into short (8-week) or long (16-week) follow-up groups. Sham operation, resection of the proximal third of the small bowel, resection of the distal third of the bowel and resection of the entire jejunum and ileum were carried out. Nineteen days before the end of the experiment the animals were transferred into a metabolic cage to analyze weight gain/loss, food intake, and fecal excretion/24 h. At the end of the experiment the animals were killed; blood samples and bowel and bone specimens were collected, length, weight, volume, density, mineral content, and fracturing energy were determined, and bone histology was examined. The calcium/phosphorus ratio, nonmineralized tissue content and the ratio fracturing energy/mean bone density were calculated.
  
  
  RESULTS
  After 8 weeks there were significant differences to the control group in body weight, weight gain, food efficiency, femur length, weight, volume, mineral content, mineral density, fracturing energy per bone volume, and bone density but not in bone calcium or magnesium. After 16 weeks there were differences in body weight, weight gain, food efficiency, femur length, weight, volume, bone mineral content and density, bone minerals, and nonmineralized tissue but not in fracturing energy; the average values of all these parameters were lower in the resected groups, and lowest in the group after resection of the entire jejunum and ileum. Bone histology showed a reduction in trabecular bone mass after long-segment small bowel resection.
  
  
  CONCLUSIONS
  Long-segment small bowel resection causes a significant loss of body weight despite of a comparable mean chow ingestion resulting in a significant decreased food efficiency. We conclude that there is no inverse relationship of bone calcium content and the fracture risk, and that there is no severe mineralization defect after long-segment small bowel resection.
authors.affiliation: Department of Surgery, University of Erlangen, Germany. sdkastl@aol.com
authors.forename: S
authors.initials: S
authors.lastname: Kastl
authors.forename: H
authors.initials: H
authors.lastname: Gepp
authors.forename: T
authors.initials: T
authors.lastname: Sommer
authors.forename: U
authors.initials: U
authors.lastname: Kotschenreuther
authors.forename: M
authors.initials: M
authors.lastname: Radespiel-Tröger
authors.forename: P O
authors.initials: PO
authors.lastname: Schwille
authors.forename: W
authors.initials: W
authors.lastname: Hohenberger
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density; D001851:Bone Diseases, Metabolic / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D002118:Calcium / Q000378:metabolism; D005269:Femur / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D007421:Intestine, Small / Q000601:surgery*; D008274:Magnesium / Q000378:metabolism; D008297:Male; D010758:Phosphorus / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D010758:Phosphorus; D008274:Magnesium; D002118:Calcium