Proteomics of allergens.
The present state of proteomics research is generally outlined and the character of allergenic compounds briefly elucidated. The principles of experimental approaches to isolation, purification, identification and characterization of allergens and to monitoring of their biological activity are described, with emphasis on the most modern methods. Selected examples are given for illustration and important results are summarized in tables.
Tichá
Marie
M
Department of Biochemistry, Charles University, Faculty of Sciences, Albertov 2030, 128 40 Prague 2, Czech Republic.
Pacáková
Vera
V
Stulík
Karel
K
D000485:Allergens / Q000378:metabolism*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D020543:Proteome
D000485:Allergens; D020543:Proteome