title: [Properties of atropine interaction with the plasma membrane of myometrial cells].
abstract: Atropine binding with some vesicular preparations of pig myometrium cellular plasmatic membranes has been estimated by means of ultracentrifugation in order of membranes precipitation as well as of two-beam spectrofotometry designed for recording UV-spectrum absorption by the residual solutions containing atropin. While utilizing Langmuir probe of sorption isotherm rectilinear sites the affinity of binding centers to atropin (34 kJ/mol) and Kd = 1 mmM was measured. Fresing-defrosting of the membranes vesicular preparations provides for the sorption increase by 28% and the affinity decrease reaching 13 kJ/mol. The hypothesis was made about the prioritative correct orientation of vesicules.
authors.affiliation: Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Danylovych
authors.forename: V A
authors.initials: VA
authors.lastname: Tuhaĭ
mesh_terms: D000818:Animals; D001285:Atropine / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D009215:Myometrium / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D014461:Ultracentrifugation
chemical_list: D001285:Atropine