[A comparative study on the syntheses of DNA, RNA and protein during in vitro organogenesis and somatic embryogenesis of Lycium barbarum L].
After the calli originating from the leaf explant of Lycium barbarum L. were selected and proliferated, the yellowish calli with same origin, similar state were transferred to O medium or E medium and the regenerative systems of organogenesis and somatic embryogenesis might form. By these systems, a comparative study on the synthetic activities of DNA, RNA and protein in the two in vitro regeneration pathways was carried out. The results were as follows: (1) Before meristemoid and embryogenic cells were formed, the synthesis of RNA was activated firstly, followed with the increase of synthesis rates of DNA and protein. During the formation of globular embryo, the synthesis rate of DNA increased quickly and then the activities of syntheses of RNA and protein reached the peak, while it was the contrary during germination of adventitious bud. (2) Components of soluble protein changed regularly. A peptide (153.6 kD) appeared during the initiation of both organogenesis and somatic embryogenesis. Several peptides disappearing gradually in the early-stage of differentiation could regenerate with the formation of shoot primordium and globular embryo. Corresponding to morphogenesis, both regenerative systems had specific peptides (84.9 kD, 46.3 kD and 44 kD, 36.2 kD) as molecular markers of its own development. In addition, the relation and mechanism of the two regenerative systems were discussed.
Hu
Z
Z
Department of Biology, Lanzhou University, Lanzhou 730000.
Ding
H B
HB
Wang
X
X
Wang
L S
LS
D046508:Culture Techniques; D018744:DNA, Plant / Q000096:biosynthesis*; D032305:Lycium / Q000254:growth & development* / Q000378:metabolism; D018515:Plant Leaves / Q000254:growth & development; D010940:Plant Proteins / Q000096:biosynthesis*; D018749:RNA, Plant / Q000096:biosynthesis*
D018744:DNA, Plant; D010940:Plant Proteins; D018749:RNA, Plant