title: Novel splice variants of amphiphysin I are expressed in retina.
abstract: Using RT-PCR-based cDNA cloning, we identified novel splice variants of amphiphysin I, termed amph Ir, that are expressed specifically in retina. In comparison with the prototype amphiphysin I, amph Ir contained two novel insertions (inserts A and B) and one deletion. Insert A is only 9 bp in length but appears to be a determinant for the retina-specific expression. In contrast, insert B is a large domain of 1740 bp and two shorter transcripts with 3'-truncated insert B were also expressed. All the insert sequences were present as unidentified exons in the amphiphysin I gene on human chromosome 7. Western blot analysis of various rat tissues with anti-insert B antibody confirmed the presence and tissue specificity of the variant proteins.
authors.affiliation: Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Terada
authors.forename: Ken
authors.initials: K
authors.lastname: Tsutsui
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Sano
authors.forename: Osamu
authors.initials: O
authors.lastname: Hosoya
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohtsuki
authors.forename: Akira
authors.initials: A
authors.lastname: Tokunaga
authors.forename: Kimiko
authors.initials: K
authors.lastname: Tsutsui
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D006801:Humans; D007438:Introns; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics*; D009928:Organ Specificity; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C075637:amphiphysin