title: Oxidant regulation of the Saccharomyces cerevisiae GSH1 gene.
abstract: Glutathione (gamma-L-glutamyl-L-cysteinylglycine) is an important antioxidant molecule, helping to protect the cell against oxidative stress. Expression of the Saccharomyces cerevisiae GSH1 gene, coding for the first enzyme involved in glutathione biosynthesis, is regulated at the level of transcription by oxidants and heavy metals. We have characterised the sequences of the GSH1 promoter responsible for the amino acid-dependent H(2)O(2) regulation of transcription. We show that there are at least two H(2)O(2)-responsive elements in the promoter, neither of which map to the putative Yap1 binding site. Our results suggest that the Yap1 protein plays an important, but indirect role in the H(2)O(2)-dependent regulation of GSH1 transcription.
authors.affiliation: Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, UK.
authors.forename: Ulla H
authors.initials: UH
authors.lastname: Dormer
authors.forename: John
authors.initials: J
authors.lastname: Westwater
authors.forename: Duncan W S
authors.initials: DW
authors.lastname: Stephen
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Jamieson
mesh_terms: D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005721:Glutamate-Cysteine Ligase; D018398:Homeodomain Proteins / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D018398:Homeodomain Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae; D006861:Hydrogen Peroxide; C490862:GSH1 protein, S cerevisiae; D005721:Glutamate-Cysteine Ligase