title: Development and validation of a liquid chromatographic method for Casiopeina IIIi in rat plasma.
abstract: A sensitive and specific liquid chromatographic method using extraction with zinc sulfate has been developed for the determination of Casiopeina IIIi and validated over the linear range 5-100 microg/ml in 1 ml of rat plasma. The analysis was performed on a Symmetry C(18) (5 microm) column. The mobile phase was methanol: 0.01 M phosphate buffer pH 6.5 (40:60, v/v). The column effluent was monitored at 262 nm. The results showed that the assay is sensitive at 5 microg/ml. Maximum intra-day coefficient of variation was 10.6%. The recovery obtained in plasma was 87.2%. The method was used to perform protein binding studies by equilibrium dialysis in rat plasma and was found to be satisfactory.
authors.affiliation: Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, 04510 México D.F., Mexico.
authors.forename: Inés
authors.initials: I
authors.lastname: Fuentes-Noriega
authors.forename: Lena
authors.initials: L
authors.lastname: Ruiz-Ramírez
authors.forename: Araceli
authors.initials: A
authors.lastname: Tovar Tovar
authors.forename: Héctor
authors.initials: H
authors.lastname: Rico-Morales
authors.forename: Isabel
authors.initials: I
authors.lastname: Gracia-Mora
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D009942:Organometallic Compounds / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; D009942:Organometallic Compounds; C461490:casiopeina IIIi