title: Nitric oxide and hydroxyl radicals initiate lipid peroxidation by NMDA receptor activation.
abstract: In this experiment, we used direct electron paramagnetic resonance (EPR) spectra to measure lipid peroxidation by hydroxyl radical (.OH), nitric oxide (.NO) and lipid radical (.L). NMDA-receptor associated lipid peroxidation is thought to act through .OH in induction of neurotoxicity. The origin of .OH generation was found to arise mainly from peroxynitrite anion produced from O(2)(-) and .NO rather than from Fenton's reaction. This study verified that .OH generation from interactive reactions between .NO and O(2)(-) initiates NMDA-induced lipid peroxidation of PC12 cells.
authors.affiliation: Department of Psychiatry, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889-1692, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Yuto
authors.initials: Y
authors.lastname: Ueda
authors.forename: Akira
authors.initials: A
authors.lastname: Nakajima
authors.forename: Hidekatsu
authors.initials: H
authors.lastname: Yokoyama
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Mitsuyama
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ohya-Nishiguchi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kamada
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004578:Electron Spin Resonance Spectroscopy; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D016202:N-Methylaspartate / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*
chemical_list: D018690:Excitatory Amino Acid Agonists; D016194:Receptors, N-Methyl-D-Aspartate; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D016202:N-Methylaspartate