title: Comparison of the resistance to infection of rifampin-bonded gelatin-sealed and silver/collagen-coated polyester prostheses.
abstract: OBJECTIVE
  The purpose of this study was to compare the efficacy of rifampin-bonded gelatin-sealed and silver acetate/collagen-coated knitted polyester prostheses for the prevention of bacteremic graft infection in an animal model.
  
  
  METHODS
  Eighteen 6.0-mm polyester grafts (length, 5.0 cm) were implanted in dogs end-to-end into the infrarenal aorta. The dogs were divided into four groups as a function of type of prosthesis implanted. The dogs in groups I (n = 3) and II (n = 3) received control gelatin-sealed or collagen-coated polyester prostheses, respectively. In group III (n = 6), the dogs received rifampin-bonded gelatin-sealed polyester prostheses. In group IV (n = 6), the dogs received silver/collagen-coated polyester prostheses. Two days after implantation, the grafts were challenged with 6 x 10(9) Staphylococcus aureus intravenously. One week after implantation, the grafts were harvested with sterile technique. Quantitative cultures were obtained from all the harvested grafts. The results were expressed as colony-forming units per cm(2) of graft material. Bacteriologic study was also performed on various tissue samples. The chi(2) test was used to compare the culture proven infection of control and antimicrobial grafts.
  
  
  RESULTS
  All the control grafts were infected with S aureus at the time of removal. Five of the six silver/collagen-coated grafts were infected, whereas none of the six rifampin-bonded gelatin-sealed grafts grew S aureus (P <.01). There was no significant difference in the number of positive culture results of organ samples between the different groups of dogs.
  
  
  CONCLUSIONS
  These results indicate that rifampin-bonded gelatin-sealed polyester grafts are significantly more resistant to bacteremic infection than are silver/collagen-coated polyester grafts in a highly challenging model.
authors.affiliation: Department of Vascular Surgery and Microbiology, Ambroise Paré University Hospital and Faculté de Médecine Paris-Ouest, René Descartes University, 9 avenue Charles de Gaulle, 92100 Boulogne, France. ogb@club-internet.fr
authors.forename: Olivier A
authors.initials: OA
authors.lastname: Goëau-Brissonnière
authors.forename: Dominique
authors.initials: D
authors.lastname: Fabre
authors.forename: Véronique
authors.initials: V
authors.lastname: Leflon-Guibout
authors.forename: Isabelle
authors.initials: I
authors.lastname: Di Centa
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Nicolas-Chanoine
authors.forename: Marc
authors.initials: M
authors.lastname: Coggia
mesh_terms: D000085:Acetates / Q000008:administration & dosage*; D000818:Animals; D001672:Biocompatible Materials; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation; D004285:Dogs; D011091:Polyesters; D016459:Prosthesis-Related Infections / Q000517:prevention & control*; D012293:Rifampin / Q000008:administration & dosage*; D018030:Silver Compounds / Q000008:administration & dosage*; D013203:Staphylococcal Infections / Q000517:prevention & control*
chemical_list: D000085:Acetates; D001672:Biocompatible Materials; D011091:Polyesters; D018030:Silver Compounds; C103100:silver acetate; D012293:Rifampin