title: Phylogeny of the fungus Spilocaea oleagina, the causal agent of peacock leaf spot in olive.
abstract: The fungus Spilocaea oleagina causes peacock leaf spot in olive. Virtually nothing is known about S. oleagina despite the loss of crop yield caused by this fungus. In order to get insight, an in vitro culture of the fungus has been established and its identity confirmed by amplified fragment length polymorphism analysis. Using this in vitro culture, we have cloned and analysed the DNA sequences of the 18S and 28S ribosomal RNA genes (rDNA) as well as the internal transcribed spacers (ITS) and 5.8S rDNA region of S. oleagina. Sequence analysis and comparison to other fungi determined that this fungus belongs to the Dothideomycetes class. We have also determined that S. oleagina is an anamorphic phase of a yet unidentified Venturia species based on phylogenetic analysis using the 28S rDNA and ITS sequences.
authors.affiliation: Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, Campus de Teatinos s/n, 29071, Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: González-Lamothe
authors.forename: Rosa
authors.initials: R
authors.lastname: Segura
authors.forename: Antonio
authors.initials: A
authors.lastname: Trapero
authors.forename: Luciana
authors.initials: L
authors.lastname: Baldoni
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Botella
authors.forename: Victoriano
authors.initials: V
authors.lastname: Valpuesta
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000472:pathogenicity; D001483:Base Sequence; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology*; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012639:Seeds / Q000821:virology
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D004279:DNA, Viral; D012339:RNA, Ribosomal, 28S