The iron-containing superoxide dismutase of Ralstonia metallidurans CH34.
The iron-containing superoxide dismutase (Fe-SOD) of Ralstonia metallidurans CH34 was purified and characterised as a homodimer of 2 x 21500 Da containing one iron atom per monomer and exhibiting all the characteristics of the prokaryotic Fe-SODs except for a higher isoelectric point. The protein was 2-fold overexpressed in the presence of selenite, zinc or paraquat. R. metallidurans CH34 was suggested to contain a gene encoding for a manganese-containing SOD located in the inducible chromate resistance operon. Whatever the culture conditions used in this study, including the presence of chromate, only a Fe-SOD, genetically distinct from the putative Mn-SOD, was detected. This Fe-SOD seems to be the only active superoxide dismutase expressed in R. metallidurans CH34.
Roux
Murielle
M
Laboratoire de Chimie et Biochimie des Centres Rédox Biologiques, CEA-Grenoble, DBMS/CB, UMR 5047 CNRS-CEA-UJF, 17 Avenue des Martyrs, 38054 Grenoble Cedex 9, France.
Covés
Jacques
J
D000421:Alcaligenes / Q000201:enzymology*; D019281:Dimerization; D004578:Electron Spin Resonance Spectroscopy; D007501:Iron / Q000032:analysis*; D007700:Kinetics; D021122:Protein Subunits; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D021122:Protein Subunits; D007501:Iron; D013482:Superoxide Dismutase