T-cell receptor bias in patients with allergic bronchopulmonary aspergillosis.
CD4(+) Th2 helper cell mediated immune responses have been shown to play a crucial role in the pathogenesis of ABPA. HLA and TCR are the candidate genes, which can influence the specificity of these responses. We have previously established a strong association of HLA DR2/5 in ABPA susceptibility. The study was designed to determine whether allergen specific T cell express a limited usage of T cell receptor (TCR) Vbeta gene repertoire in ABPA and to find an association of susceptible HLA-DR determinants with the identified TCR gene segments. TCR Vbeta typing was performed on antigen specific T cell lines from 14 ABPA and 12 nonABPA patients. The majority of ABPA patients (86%) expressed allergen specific T cells with Vbeta13 genes indicating its role in susceptibility, whereas in nonABPA controls, Vbeta1 genes T cell repertoires were predominantly expressed. The unrestricted pattern of Vbeta gene amplification seen before antigen stimulation suggests an oligoclonal expansion of a specific T cell population in response to the allergen Asp f 1 in ABPA and nonABPA patients. The increased usage of Vbeta13 in ABPA and Vbeta1 in nonABPA indicates their importance in susceptibility and resistance, respectively.
Chauhan
Bela
B
Department of Internal Medicine, Saint Louis University School of Medicine, St. Louis, MO 63104, USA.
Hutcheson
Patricia S
PS
Slavin
Raymond G
RG
Bellone
Clifford J
CJ
D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant; D001229:Aspergillosis, Allergic Bronchopulmonary / Q000097:blood / Q000276:immunology*; D002478:Cells, Cultured; D005656:Fungal Proteins / Q000276:immunology*; D015870:Gene Expression; D015802:HLA-DR2 Antigen / Q000276:immunology; D015805:HLA-DR5 Antigen / Q000276:immunology; D006801:Humans; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
C081947:ASPF1 protein, Aspergillus fumigatus; D000485:Allergens; D052179:Antigens, Plant; D005656:Fungal Proteins; D015802:HLA-DR2 Antigen; D015805:HLA-DR5 Antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta