title: Direct identification of stylet secreted proteins from root-knot nematodes by a proteomic approach.
abstract: The stylet secretions produced by plant parasitic root-knot nematodes are thought to be pathogenicity factors involved in the invasion of the root tissue and in the induction and maintenance of feeding cells. A new procedure was established that allowed the direct qualitative analysis of proteins secreted by Meloidogyne incognita infective juveniles. Purified proteins whose isoelectric point (pI) ranged from 5.0 to 7.5 were separated by two-dimensional (2D) electrophoresis and the seven most abundant proteins were identified by micro-sequencing. A calreticulin (CRT) was isolated and transcription of its gene in infective juveniles and adults was demonstrated. Moreover, evidence for expression of the CRT in the subventral oesophageal glands of infective juveniles was obtained. The potential roles of this secreted protein in pathogenesis and the advantages of developing this strategy to obtain new insights into plant-nematode interactions are discussed.
authors.affiliation: INRA, Unité Interactions Plantes-Microorganismes et Santé Végétale, 123 bld Francis Meilland BP2078, 06606 Antibes Cedex, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Jaubert
authors.forename: Terence Neil
authors.initials: TN
authors.lastname: Ledger
authors.forename: Jean Baptiste
authors.initials: JB
authors.lastname: Laffaire
authors.forename: Christine
authors.initials: C
authors.lastname: Piotte
authors.forename: Pierre
authors.initials: P
authors.lastname: Abad
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Rosso
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D037282:Calreticulin; D015180:Electrophoresis, Gel, Two-Dimensional; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000469:parasitology*; D020543:Proteome; D012118:Resorcinols / Q000494:pharmacology; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014158:Transcription, Genetic; D014431:Tylenchoidea / Q000187:drug effects / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D002135:Calcium-Binding Proteins; D037282:Calreticulin; D015801:Helminth Proteins; D020543:Proteome; D012118:Resorcinols; D012261:Ribonucleoproteins; C031389:resorcinol