title: Influences of olfactory ensheathing cells transplantation on axonal regeneration in spinal cord of adult rats.
abstract: OBJECTIVE
  To observe whether olfactory ensheathing cells could be used to promote axonal regeneration in a spontaneously nonregenerating system.
  
  
  METHODS
  After laminectomy at the lower thoracic level, the spinal cords of adult rats were exposed and completely transected at T10. A suspension of ensheathing cells was injected into the lesion site in 12 adult rats, and control D/F-12 (1:1 mixture of DMEM and Ham's F-12) was injected in 12 adult rats. Six weeks and ten weeks after cell transplantation, the rats were evaluated by climbing test and motor evoked potentials (MEPs) monitoring. The samples were procured and studied with histologicl and immunohistochemical methods.
  
  
  RESULTS
  At the 6th week after cell transplantation, all the rats in both the transplanted and control groups were paraplegic and the MEPs could not be recorded. At the 10th week after cell transplantation, of 7 rats in the control group, 2 rats had muscles' contraction of the lower extremities, 2 rats had hips and/or knees' active movement; and 5 rats' MEPs could be recorded in the hind limbs in the transplanted group (n=7). None of the rats in the control group had functional improvement and no MEPs recorded (n=7). Numerous regenerating axons were observed through the transplantation and continued to regenerate into the denervated host tract. Cell labelling using anti-Myelin Basic Protein (MBP) and anti-Nerve Growth Factor Receptor (anti-NGFR) indicated that the regenerated axons were derived from the appropriate neuronal source and that donor cells migrated into the denervated host tract. But axonal degeneration existed and regenerating axons were not observed within the spinal cords of the adult rats with only D/F-12 injection.
  
  
  CONCLUSIONS
  The axonal regeneration in the transected adult rat spinal cord is possible after ensheathing cells transplantation.
authors.affiliation: Department of Orthopaedics, Memorial Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510120, China. shenh.y.@163.com
authors.forename: Huiyong
authors.initials: H
authors.lastname: Shen
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Wu
authors.forename: Yantao
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhian
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D016380:Brain Tissue Transplantation; D017690:Cell Transplantation; D008297:Male; D009416:Nerve Regeneration; D009830:Olfactory Bulb / Q000166:cytology* / Q000637:transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000503:physiopathology / Q000601:surgery*