Phototrophic transformation of phenol to 4-hydroxyphenylacetate by Rhodopseudomonas palustris.
Newly isolated and culture collection strains of Rhodopseudomonas palustris were able to transform phenol to 4-hydroxyphenylacetate under phototrophic conditions in the presence of acetate, malate, benzoate, or cinnamate as growth substrates. The reaction was examined with uniformly (14)C-labelled phenol and the product was identified by HPLC retention time, UV-scans, and (1)H- and (13)C-NMR analysis. The transformation reaction was detectable in cell-free extracts in the presence of NAD(+) and acetyl-CoA. For further degradation of 4-hydroxyphenylacetate by R. palustris, low partial pressures of oxygen were essential, presumably for aerobic aromatic ring fission reactions by mono- and di-oxygenases.
Noh
U
U
Applied Microbiology, University of Saarbrücken, PO Box 151150, 66041 Saarbrücken, Germany.
Heck
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S
Giffhorn
F
F
Kohring
G-W
GW
D009682:Magnetic Resonance Spectroscopy; D010100:Oxygen / Q000378:metabolism; D019800:Phenol / Q000378:metabolism*; D010648:Phenylacetates / Q000378:metabolism*; D012241:Rhodopseudomonas / Q000378:metabolism*
D010648:Phenylacetates; C026246:4-hydroxyphenylacetate; D019800:Phenol; D010100:Oxygen