title: Crystallization of the AAA domain of the ATP-dependent protease FtsH of Escherichia coli.
abstract: FtsH is a membrane-anchored ATP-dependent protease that degrades misfolded or misassembled membrane proteins as well as a subset of cytoplasmic regulatory proteins. It belongs to the family of AAA(+) ATPases with roles in diverse cellular processes. The ATPase domain of FtsH from Escherichia coli has been crystallized from ammonium sulfate solutions and crystals diffracting to 1.5 A resolution have been obtained.
authors.affiliation: Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, England.
authors.forename: Szymon
authors.initials: S
authors.lastname: Krzywda
authors.forename: Andrzej M
authors.initials: AM
authors.lastname: Brzozowski
authors.forename: Kiyonobu
authors.initials: K
authors.lastname: Karata
authors.forename: Teru
authors.initials: T
authors.lastname: Ogura
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Wilkinson
mesh_terms: D049069:ATP-Dependent Proteases; D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012697:Serine Endopeptidases / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D049069:ATP-Dependent Proteases; C513383:FtsH protein, E coli; D012697:Serine Endopeptidases