[Determination of 7 bio-active alkaloids in Stephania plants by RP-HPLC].
Seven bio-active alkaloids (stepholidine, sinoacutine, isocorydine, l-tetrahydropalmatine, crebanine, fanchinoline and tetrandrine) in Stephania plants were determined by RP-HPLC, using UV detection (282 nm) and gradient elution. The reversed phase system consisted of ODS column and methanol-water-triethylamine as mobile phase. The flow rate was 1.0 ml.min-1. Good linearity between peak heights and concentrations of the alkaloids was obtained in the concentration range. The HPLC method proved accurate, precise and sensitive. The results showed that there were some differences in the occurrence and content of the alkaloids between various species and between the same species from different habitats and collecting time. Based on the results, some species with high content of the 7 bio-active alkaloids were selected. The study provided some useful information for the utilization of medicinal plant resources in the genus Stephania.
Huang
J
J
Department of Pharmacognosy, School of Pharmacy, Shanghai Medical University, Shanghai 200032.
Guo
J
J
Duan
G
G
D000470:Alkaloids / Q000032:analysis; D001060:Aporphines / Q000032:analysis; D044182:Benzylisoquinolines; D001599:Berberine / Q000031:analogs & derivatives* / Q000032:analysis*; D001600:Berberine Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D009019:Morphinans / Q000032:analysis; D013045:Species Specificity; D031607:Stephania / Q000737:chemistry* / Q000145:classification
D000470:Alkaloids; D001060:Aporphines; D044182:Benzylisoquinolines; D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; D009019:Morphinans; D001599:Berberine; C010513:stepholidine; C061009:crebanine; C030168:isocorydine; C014215:tetrahydropalmatine; C009270:salutaridine; C060802:fangchinoline