Med, a cell-surface localized protein regulating a competence transcription factor gene, comK, in Bacillus subtilis.
Med was found as a positive regulator for comK, a master regulator for late competence genes. It was found by Western analysis that the ComK level was decreased in a med mutant. Experiments using an alkaline phosphatase fusion with Med and Western analysis of Med were done because a putative lipo-modification signal is found at the N-terminus of Med. The results obtained are consistent with the localization of Med at the cell surface. An implication of the cell-surface localization of Med is discussed in terms of comK regulation.
Ogura
Mitsuo
M
Department of Marine Science and Technology, Tokai University, Shimizu, Shizuoka, Japan. oguram@scc.u-tokai.ac.jp
Hashimoto
Hitoshi
H
Tanaka
Teruo
T
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D016384:Consensus Sequence; D017931:DNA Primers; D015964:Gene Expression Regulation, Bacterial; D008024:Ligands; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics*
D001426:Bacterial Proteins; D017931:DNA Primers; D008024:Ligands; D015534:Trans-Activators; D014157:Transcription Factors; C087449:comK protein, Bacillus subtilis