Activities of overt and latent diacylglycerol acyltransferases (DGATs I and II) in liver microsomes of ob/ob mice.
The effect of the ob/ob mutation on microsomal overt and latent acyl CoA:diacylglycerol acyl transferase activities (DGATs I and II) in liver microsomes of mice was investigated. Overt and latent hepatic DGAT activities for 16-week-old lean Ob/? mice were 0.42+/-0.14 and 1.57+/-0.04 micromol/mg/min, respectively. For ob/ob mice DGAT I and II activities were 1.17+/-0.28 and 3.09+/-0.78 micromol/mg/min, respectively. The hepatic triacylglycerol (TAG) concentration of 16-week-old mice was increased three-fold in ob/ob mice relative to those in lean controls. The data suggest that the increased rate of hepatic TAG secretion and intracellular accumulation in ob/ob mice is accompanied by parallel increases in the activities of both DGATs.
Waterman
I J
IJ
Cell Biochemistry, Hannah Research Institute, Ayr, Scotland, UK. watermani@hri.sari.ac.uk
Zammit
V A
VA
D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D051048:Diacylglycerol O-Acyltransferase; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D008862:Microsomes, Liver / Q000201:enzymology*; D009154:Mutation; D009765:Obesity / Q000201:enzymology*; D014280:Triglycerides / Q000378:metabolism
D007527:Isoenzymes; D014280:Triglycerides; D000217:Acyltransferases; C494882:Dgat1 protein, mouse; D051048:Diacylglycerol O-Acyltransferase