Characteristics of Na(+)-dependent intestinal nucleoside transport in the pig.
Since the capacity of nucleic acid digestion and absorption appears to be comparatively high in the pig, we investigated the properties of transport of (3)H-labelled nucleosides across the porcine intestinal brush border membrane (BBM) using BBM vesicles isolated from the small intestine of slaughter pigs. In the presence of a transmembrane Na(+) gradient, uridine, thymidine and guanosine transiently accumulated in the vesicular lumen beyond the equilibrium (60 min) value suggesting the presence of Na(+)/nucleoside cotransporters in the BBM. The findings of inhibitory studies are consistent with the presence of two Na(+)-dependent nucleoside transporters with overlapping substrate specificity, one for pyrimidine nucleosides (N2) and one for purine nucleosides (N1). Guanosine appeared to be a specific substrate for N1, while this applies to thymidine for N2. Transport of thymidine and guanosine were also inhibited by 2 mmol/l D-glucose and alpha-methyl-D-glucoside. The maximal transport capacity (V(max)) for Na(+)-dependent thymidine and guanosine transport were much higher than reported for other monogastric species. Unlike in other species tested, there was no proximal-to-distal gradient, neither in nucleoside transport activity nor in the inhibition of nucleoside transport by monosaccharides in the porcine small intestine. The high intestinal nucleoside transport activity may contribute to the high digestive capacity for nucleic acids in the pig.
Scharrer
E
E
Institut of Veterinary Physiology, University of Zurich, Winterthurerstrasse 260, CH-8057 Zürich, Switzerland. scharrer@vetphys.unizh.ch
Rech
K S
KS
Grenacher
B
B
D000818:Animals; D005947:Glucose / Q000378:metabolism; D006151:Guanosine / Q000493:pharmacokinetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D009705:Nucleosides / Q000493:pharmacokinetics*; D012964:Sodium / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*; D013936:Thymidine / Q000493:pharmacokinetics; D014316:Tritium; D014529:Uridine / Q000493:pharmacokinetics
D026901:Membrane Transport Proteins; D009705:Nucleosides; C065171:cif nucleoside transporter; D014316:Tritium; D006151:Guanosine; D012964:Sodium; D005947:Glucose; D013936:Thymidine; D014529:Uridine