Effects of the Superoxide Anionic Radicals to the Cultured Cerebral Cortex Neuron of Newborn Rat.
The effect of the superoxide anionic radicals to the cultured cerebral cortex neuron of newborn rat was investigated by using xanthine oxidase/xanthine. The results are as follows. The growth of neuron was inhibited ATPase activity decreased to 4.223 &mgr;mol/(mg.min(-1))(Pr) total cellular carbonyl increased to 295.40 &mgr;mol/g wet weight lipid peroxides(LPO) increased to 4.87 nmol/g(Pr) membrane lipid fluidity decreased (P value 0.398) in nuclear DNA, thesingle strand breaks(SSB) increased in the single cell gel assay(SCG), DNA migration was 7.35 mm ݣ subcellular structures of the neuron changed (mitochondria were swelling, etc.) and SOD gene expression of the neuron increased (the SOD activity and the SOD content increased, SOD mRNA content increased). These suggest that the superoxide anionic radicals could damage proteins, nucleic acids, lipids and subcellular structures in neurons.
Jiang
Zhao-Feng
ZF
Department of Biochemistry, College of Arts and Sciences, Beijing Union University, Beijing 100083, China. zfjiang@163bj.com
He
Xiang-Ping
XP
Liu
Chuan-Hui
CH
Yang
Han-Yi
HY