Diversity of streptomycetes in water-damaged building materials based on 16S rDNA sequences.
OBJECTIVE
The diversity of streptomycetes in two different types of water-damaged building materials was investigated.


RESULTS
Direct PCR amplification of 16S rDNA from DNA isolated from building materials, cloning of the fragments and sequence analysis were used. In the phylogenetic analysis of the variable gamma region of the PCR amplification products, the sequences affiliated with five groups.


CONCLUSIONS
Several different sequences were found in both materials, suggesting the presence of several species. Also, previously unknown sequences were detected, although all the sequences clustered together with sequences of known species.


CONCLUSIONS
Streptomycetes are known as indicators for moisture and mould damage in buildings and potential health risk, but their diversity in indoor environments is still unknown.
Rintala
H
H
National Public Health Institute (KTL), Laboratory of Environmental Microbiology, Kuopio, Finland. helena.rintala@ktl.fi
Nevalainen
A
A
Suutari
M
M
D003001:Cloning, Molecular; D005430:Floors and Floorcoverings; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014867:Water / Q000009:adverse effects
D012336:RNA, Ribosomal, 16S; D014867:Water