Fragmentation and dispersal of the pericentriolar Golgi complex is required for entry into mitosis in mammalian cells.
The pericentriolar Golgi stacks are fragmented and found dispersed in mitotic mammalian cells. Addition of an antibody to the Golgi-associated protein GRASP65 inhibited Golgi fragmentation by mitotic cytosol in permeabilized cells. Microinjecting this antibody or the C-terminal fragment of GRASP65, which contains the antibody binding site, into normal rat kidney cells prevented entry into mitosis. Under these conditions the cells had completed S phase but were not in the prophase stage of mitosis. Fragmentation of the Golgi apparatus by nocodazole or Brefeldin A treatment prior to or post microinjection of the anti-GRASP65 antibody alleviated the block in mitotic entry. Based on our findings, we suggest that the pericentriolar Golgi organization is a sensor for controlling entry into mitosis in mammalian cells.
Sütterlin
Christine
C
Cell and Developmental Biology Department, Division of Biology, University of California, San Diego, La Jolla 92093, USA.
Hsu
Pattie
P
Mallabiabarrena
Arrate
A
Malhotra
Vivek
V
D000595:Amino Acid Sequence; D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D001665:Binding Sites; D015415:Biomarkers; D020126:Brefeldin A / Q000494:pharmacology; D002478:Cells, Cultured; D002502:Centrioles / Q000378:metabolism / Q000502:physiology*; D000939:Epitopes / Q000378:metabolism; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D000077614:Golgi Matrix Proteins; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects / Q000502:physiology*; D015739:Nocodazole / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism
D015415:Biomarkers; D000939:Epitopes; D000077614:Golgi Matrix Proteins; C491577:Gorasp1 protein, rat; D008565:Membrane Proteins; D011994:Recombinant Proteins; D020126:Brefeldin A; D016590:Aphidicolin; D015739:Nocodazole