[Estimation of pH-dependence of calcium binding with calmodulin].
A methodical approach to estimating calmodulin Ca(2+)-binding properties based on its interaction with highly porous watman and consequent 45Ca2+ binding was proposed. At changing pH from 6.5 until 7.5 the affinity of Ca2+ to calmodulin increases in 4.3-fold. The article displays a model of mechanism for Ca(2+)-binding with calmodulin where the dissociation of H+ from Ca(2+)-binding sites is a limited stage of the process.
Danylovych
Iu V
IuV
Palladin Institute of Biochemistry of NAS of Ukraine, Kyiv. kinet@biochem.kiev.ua
Tuhaĭ
V A
VA
D000818:Animals; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D011485:Protein Binding
D002147:Calmodulin; D002118:Calcium