Identification of C3a and N-truncated C3a as vascular permeability-enhancing factors from the exudate of chronic phase of carrageenan-induced inflammation in rats.
Two basic proteins enhancing vascular permeability have been purified from the exudate of the chronic phase of carrageenan-induced inflammation in rats. One major and one minor peak on reversed-phase HPLC showed molecular masses of 9.3 kDa and 7.6 kDa, respectively, on sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. NH2-terminal amino acid sequencing analysis of the purified proteins revealed that the major peak is identical to C3a, while the main sequence of the minor peak is identical to NH2-terminal 11 amino acids truncated C3a. In addition, plasmin was able to cleave C3a into the N-truncated C3a. Intradermal injection of both purified C3a and N-truncated C3a into rat skin enhanced vascular permeability, and the increased permeability was suppressed by the pretreatment with cyproheptadine. Our results suggest that the purified C3a and N-truncated C3a have the characteristics of anaphylatoxins and may contribute to exudation in the chronic phase of carrageenan-induced inflammation in rats.
Miyama
Katsuyoshi
K
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Takano
Katsuhiko
K
Atsumi
Ikuyo
I
Nakagawa
Hideo
H
D000595:Amino Acid Sequence; D000818:Animals; D002199:Capillary Permeability / Q000502:physiology*; D002351:Carrageenan; D002851:Chromatography, High Pressure Liquid; D015926:Complement C3a / Q000502:physiology*; D003533:Cyproheptadine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005122:Exudates and Transudates / Q000737:chemistry*; D005341:Fibrinolysin / Q000378:metabolism; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000502:physiology; D012867:Skin / Q000098:blood supply
D006634:Histamine H1 Antagonists; D003533:Cyproheptadine; D015926:Complement C3a; D002351:Carrageenan; D005341:Fibrinolysin