title: IR spectra and IR spectral maps of individual normal and cancerous cells.
abstract: The IR microspectra for individual normal and cancerous cells are reported at a spatial resolution that permits a distinction between the nuclear and cytoplasmic regions. The observed spectra reveal large differences in the spectral contributions of RNA, DNA, and phospholipids: metabolically inactive cells show spectral patterns of proteins only, whereas actively dividing cells also show spectral signatures of nucleic acids and phospholipids. These spectral variations are independent of the state of health of a cell.
authors.affiliation: Department of Chemistry, City University of New York, Hunter College, 695 Park Avenue, New York, New York 10021, USA. mdiemhc@aol.com
authors.forename: Max
authors.initials: M
authors.lastname: Diem
authors.forename: Luis
authors.initials: L
authors.lastname: Chiriboga
authors.forename: Peter
authors.initials: P
authors.lastname: Lasch
authors.forename: Anthony
authors.initials: A
authors.lastname: Pacifico
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D016193:G1 Phase; D016195:G2 Phase; D015726:Giant Cells / Q000378:metabolism; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D016196:S Phase; D012509:Sarcoma / Q000378:metabolism; D013055:Spectrophotometry, Infrared / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism
chemical_list: D010743:Phospholipids; D012313:RNA; D004247:DNA