title: Slug is a novel downstream target of MyoD. Temporal profiling in muscle regeneration.
abstract: Temporal expression profiling was utilized to define transcriptional regulatory pathways in vivo in a mouse muscle regeneration model. Potential downstream targets of MyoD were identified by temporal expression, promoter data base mining, and gel shift assays; Slug and calpain 6 were identified as novel MyoD targets. Slug, a member of the snail/slug family of zinc finger transcriptional repressors critical for mesoderm/ectoderm development, was further shown to be a downstream target by using promoter/reporter constructs and demonstration of defective muscle regeneration in Slug null mice.
authors.affiliation: Research Center for Genetic Medicine, Children's National Medical Center, and Genetics Program, George Washington University, Washington, D. C. 20010, USA.
authors.forename: Po
authors.initials: P
authors.lastname: Zhao
authors.forename: Simona
authors.initials: S
authors.lastname: Iezzi
authors.forename: Ethan
authors.initials: E
authors.lastname: Carver
authors.forename: Devin
authors.initials: D
authors.lastname: Dressman
authors.forename: Thomas
authors.initials: T
authors.lastname: Gridley
authors.forename: Vittorio
authors.initials: V
authors.lastname: Sartorelli
authors.forename: Eric P
authors.initials: EP
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology*; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012038:Regeneration / Q000235:genetics*; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D017570:MyoD Protein; C000604407:SNAI1 protein, human; C000604449:Snai2 protein, mouse; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors