Bifunctional phosphomannose isomerase/GDP-D-mannose pyrophosphorylase is the point of control for GDP-D-mannose biosynthesis in Helicobacter pylori.
In this report a recombinant bifunctional phosphomannose isomerase/GDP-D-mannose pyrophosphorylase from Helicobacter pylori has been studied. The enzyme catalyzes the first and third steps of GDP-D-mannose biosynthesis from D-fructose-6-phosphate. The first step, isomerization from D-fructose-6-phosphate to D-mannose-6-phosphate, is found to be rate-limiting in GDP-D-mannose biosynthesis due to feedback inhibition. The inhibition is of non-competitive (mixed) type. As the enzyme is found only in bacteria probably participating in capsular polysaccharide biosynthesis, it could be a specific therapeutic target against bacterial infection.
Wu
Bingyuan
B
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Zhang
Yingxin
Y
Zheng
Rong
R
Guo
Cuiwen
C
Wang
Peng George
PG
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D017124:Conserved Sequence; D004789:Enzyme Activation / Q000187:drug effects; D025461:Feedback, Physiological / Q000502:physiology; D005636:Fructosephosphates / Q000378:metabolism; D006155:Guanosine Diphosphate Mannose / Q000096:biosynthesis* / Q000494:pharmacology; D016480:Helicobacter pylori / Q000201:enzymology*; D008359:Mannose-6-Phosphate Isomerase / Q000235:genetics / Q000378:metabolism*; D008360:Mannosephosphates / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D005636:Fructosephosphates; D008360:Mannosephosphates; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; D006155:Guanosine Diphosphate Mannose; C027693:mannose-6-phosphate; C027618:fructose-6-phosphate; D009713:Nucleotidyltransferases; C026917:mannose 1-phosphate guanylyltransferase; D008359:Mannose-6-Phosphate Isomerase