title: TEM-71, a novel plasmid-encoded, extended-spectrum beta-lactamase produced by a clinical isolate of Klebsiella pneumoniae.
abstract: TEM-71, a novel extended-spectrum beta-lactamase from a Klebsiella pneumoniae clinical isolate, had an isoelectric point of 6.0 and a substrate profile showing preferential hydrolysis of cefotaxime over ceftazidime. It differed from TEM-1 by two substitutions, Gly238Ser and Glu240Lys, and was under the control of the strong P4 promoter.
authors.affiliation: Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. Jkr1@cdc.gov
authors.forename: J Kamile
authors.initials: JK
authors.lastname: Rasheed
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Anderson
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Queenan
authors.forename: James W
authors.initials: JW
authors.lastname: Biddle
authors.forename: Antonio
authors.initials: A
authors.lastname: Oliver
authors.forename: George A
authors.initials: GA
authors.lastname: Jacoby
authors.forename: Karen
authors.initials: K
authors.lastname: Bush
authors.forename: Fred C
authors.initials: FC
authors.lastname: Tenover
mesh_terms: D019943:Amino Acid Substitution; D002439:Cefotaxime / Q000378:metabolism; D002442:Ceftazidime / Q000378:metabolism; D018550:Cephalosporin Resistance / Q000235:genetics*; D002511:Cephalosporins / Q000378:metabolism / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D007525:Isoelectric Focusing; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D002511:Cephalosporins; D004269:DNA, Bacterial; D002442:Ceftazidime; C466678:TEM-71 beta-lactamase; D001618:beta-Lactamases; D002439:Cefotaxime