[Specific antibodies against recombinant MSP1 of Plasmodium falciparum strongly inhibit the parasite growth in vitro].
In order to produce large amounts of protein for vaccine trials, a synthetic msp1-42 gene was inserted into Pichia pastoris expression vector and the plasmid was introduced into Pichia pastoris SMD1168 by electroporation. The expressed MSP1-42 was secreted into the protein-free medium. To measure the conformational properties of MSP1-42,16 monoclonal antibodies (11 recognizing conformational epitopes) were allowed to interact with the Pichia-derived MSP1-42, and all antibodies specific for conserved and K1 protype interacted with the protein. Interestingly, three monoclonal antibodies (e.g. 9.8, 13.1 and 7.3), that were shown not to interact with CHO-derived MSP1, could interact with the Pichia-derived MSP1-42. Rabbits were immunized with recombinant MSP1-42 formulated with CFA adjuvant four times. The rabbits were bled on the day 3 after last immunization, and total IgG isolated by protein A column from the immunized rabbits was shown to strongly inhibit the parasite growth in vitro dose-dependently, whereas IgG from rabbit with adjuvant had no inhibition.
Zhang
D M
DM
Department of Etiological Biology of Second Military Medical University, Shanghai 200433, China. malaria@guomai.sh.cn
Pan
W Q
WQ
Lu
D R
DR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000918:Antibody Specificity; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D020066:Merozoite Surface Protein 1 / Q000276:immunology*; D021261:Parasitic Sensitivity Tests; D010446:Peptide Fragments / Q000276:immunology; D010843:Pichia / Q000235:genetics; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000981:Antiprotozoal Agents; D020066:Merozoite Surface Protein 1; D010446:Peptide Fragments; D011994:Recombinant Proteins