Polypeptide synthesis directed by DNA as a messenger in cell-free polypeptide synthesis by extreme thermophiles, Thermus thermophilus HB27 and Sulfolobus tokodaii strain 7.
Polypeptide synthesis at high temperature directed by single strand DNA as a messenger was investigated using cell-free extracts of an extremely thermophilic bacterium, Thermus thermophilus strain HB27, and a hyperthermophilic, acidophilic archaeon, Sulfolobus tokodaii strain 7. Aminoglycoside antibiotics enhanced the reaction; neomycin stimulated it most effectively when the extract of the thermophilic bacterium was used, and paromomycin was the best among the antibiotics tested for the extract of the hyperthermophilic archaeon. A common correlation was found between the stimulation of DNA-directed polypeptide synthesis and the misreading rate in RNA-directed polypeptide synthesis. Spermine stimulated the reaction directed by DNA like in the case of poly(Phe) synthesis directed by poly (rU). The cell-free systems can be used for direct production of proteins from genes in high throughput studies on the structural genomics of thermophilus.
Uzawa
Taketoshi
T
Department of Life Science, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan. uzawa@cc.osaka-kyoiku.ac.jp
Yamagishi
Akihiko
A
Oshima
Tairo
T
D003570:Cytidine Triphosphate / Q000494:pharmacology; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D003609:Dactinomycin / Q000494:pharmacology; D010452:Peptide Biosynthesis / Q000187:drug effects / Q000502:physiology*; D014176:Protein Biosynthesis; D016944:Sulfolobus / Q000378:metabolism*; D016963:Thermus thermophilus / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000494:pharmacology
D003609:Dactinomycin; D003570:Cytidine Triphosphate; D004247:DNA; D014544:Uridine Triphosphate