title: Optimum conditions for transformed Panax ginseng hairy roots in flask culture.
abstract: Panax ginseng hairy roots were transformed by Agrobacterium rhizogenes KTCT 2744. They showed an active branching pattern and fast growth in hormone-free medium, and good growth at 23 degrees C, pH 5.8, 1/2 MS medium, and 3% sucrose. Sucrose provided the highest growth among seven carbon sources tested. Six complex media were also tested. In the combined sugar study, hairy roots grew better on sucrose without glucose or fructose than with glucose or fructose. In the 1/2 MS basal medium, 30 mM in nitrogen and 0.62 mM phosphate salt concentration was the optimum. The growth ratio was maximal at an inoculum size of 0.4% (w/v). Crude saponin and polysaccharide levels were also measured.
authors.affiliation: Faculty of Chemical Engineering, Chonnam National University, Kwangju, Korea.
authors.forename: Gwi-Taek
authors.initials: GT
authors.lastname: Jeong
authors.forename: Don-Hee
authors.initials: DH
authors.lastname: Park
authors.forename: Hwa-Won
authors.initials: HW
authors.lastname: Ryu
authors.forename: Woo-Tai
authors.initials: WT
authors.lastname: Lee
authors.forename: Kyungmoon
authors.initials: K
authors.lastname: Park
authors.forename: Choon-Hyoung
authors.initials: CH
authors.lastname: Kang
authors.forename: Baik
authors.initials: B
authors.lastname: Hwang
authors.forename: Je-Chang
authors.initials: JC
authors.lastname: Woo
mesh_terms: D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D003470:Culture Media; D007700:Kinetics; D007785:Lactose / Q000378:metabolism; D009005:Monosaccharides / Q000378:metabolism; D005894:Panax / Q000254:growth & development* / Q000382:microbiology; D010710:Phosphates / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development* / Q000382:microbiology; D011134:Polysaccharides / Q000378:metabolism; D012231:Rhizobium / Q000378:metabolism*; D012503:Saponins / Q000302:isolation & purification / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
chemical_list: D003470:Culture Media; D009005:Monosaccharides; D010710:Phosphates; D011134:Polysaccharides; D012503:Saponins; D013395:Sucrose; D007785:Lactose