Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34+ cells with small- and large-scale Miltenyi cell sorting system.
The purging efficacy of the Miltenyi sorting system was evaluated by qualitative and TaqMan quantitative polymerase chain reaction (PCR) in myeloma patients, using immunoglobulin genes. After small-scale selection, qualitative PCR showed that in 6 of 12 leukaphereses myeloma cells were no longer detectable. Envisaging a possible clinical application, the leukaphereses from three patients underwent large-scale selection. Qualitative PCR showed that myeloma cells were still detectable. Quantitative PCR, performed in two patients, showed a tumour depletion of 1 and 2 logs respectively. Although numbers are small, the promising results obtained with small-scale selection were not reproduced in large-scale experiments.
Voena
Claudia
C
Department of Haematology, Laboratory of Molecular Haematology, Istituto H.S. Raffaele, Milan, Italy.
Locatelli
Giovanni
G
Castellino
Claudia
C
Omedè
Paola
P
Ladetto
Marco
M
Zappone
Elisabetta
E
Milani
Raffaella
R
Perfetti
Vittorio
V
Boccadoro
Mario
M
Pileri
Alessandro
A
Lusso
Paolo
P
Villa
Chiara
C
Malnati
Mauro
M
Corradini
Paolo
P
D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis*; D016465:Bone Marrow Purging / Q000379:methods*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D018189:Immunomagnetic Separation; D007937:Leukapheresis; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000473:pathology* / Q000628:therapy; D018365:Neoplasm, Residual; D016133:Polymerase Chain Reaction
D018952:Antigens, CD34