title: Amniotic membrane transplantation for reconstruction after excision of large ocular surface neoplasias.
abstract: OBJECTIVE
  To evaluate the clinical outcome of patients in whom ocular surface reconstruction was performed using amniotic membrane transplantation (AMT) after the excision of large (>20 mm square) ocular surface neoplasias (OSN).
  
  
  METHODS
  A non-comparative interventional case series. In 16 eyes of 16 patients, excision of large OSN including conjunctival intraepithelial neoplasia (CIN), primary acquired melanosis, and malignant melanoma was followed by adjunctive cryotherapy and suturing of a single layer of amniotic membrane (AM) with the basement membrane side facing up to the healthy bordering tissue. Epithelial healing, complications, and tumour recurrences were analysed.
  
  
  RESULTS
  During a mean follow up of 23.7 (SD 11, range 11-43) months, ocular surface healing was rapid and complete in all cases. One complication of pyogenic granuloma was noted. Tumour recurrence occurred in one out of 10 CIN cases (10%), no recurrences were observed in the patients with melanotic lesions.
  
  
  CONCLUSIONS
  AMT in lieu of conjunctival or mucosal autograft is an effective substrate for reconstructing the ocular surface following excision of large OSN. AMT is effective in managing large OSN by avoiding the complications that may be associated with conventional removal, specifically in cases where the limbal architecture is destroyed by surgical resection or adjuvant therapies.
authors.affiliation: Department of Ophthalmology, Bascom Palmer Eye Institute, FL, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Espana
authors.forename: P
authors.initials: P
authors.lastname: Prabhasawat
authors.forename: M
authors.initials: M
authors.lastname: Grueterich
authors.forename: A
authors.initials: A
authors.lastname: Solomon
authors.forename: S C G
authors.initials: SC
authors.lastname: Tseng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000650:Amnion / Q000637:transplantation*; D001684:Biological Dressings; D002278:Carcinoma in Situ / Q000601:surgery; D003131:Combined Modality Therapy; D003230:Conjunctival Neoplasms / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008545:Melanoma / Q000601:surgery; D008548:Melanosis / Q000601:surgery; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D014945:Wound Healing