An LRR receptor kinase involved in perception of a peptide plant hormone, phytosulfokine.
The sulfated peptide phytosulfokine (PSK) is an intercellular signal that plays a key role in cellular dedifferentiation and proliferation in plants. Using ligand-based affinity chromatography, we purified a 120-kilodalton membrane protein, specifically interacting with PSK, from carrot microsomal fractions. The corresponding complementary DNA encodes a 1021-amino acid receptor kinase that contains extracellular leucine-rich repeats, a single transmembrane domain, and a cytoplasmic kinase domain. Overexpression of this receptor kinase in carrot cells caused enhanced callus growth in response to PSK and a substantial increase in the number of tritium-labeled PSK binding sites, suggesting that PSK and this receptor kinase act as a ligand-receptor pair.
Matsubayashi
Yoshikatsu
Y
Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan. matsu@agr.nagoya-u.ac.jp
Ogawa
Mari
M
Morita
Akiko
A
Sakagami
Youji
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D001667:Binding, Competitive; D002460:Cell Line; D002846:Chromatography, Affinity; D018076:DNA, Complementary; D018552:Daucus carota / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D017343:Genes, Plant; D006031:Glycosylation; D007930:Leucine; D008024:Ligands; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D036361:Peptide Hormones; D010937:Plant Growth Regulators; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020449:Repetitive Sequences, Amino Acid
D018076:DNA, Complementary; D008024:Ligands; C100975:PSK-alpha protein, plant; D036361:Peptide Hormones; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C409473:phytosulfokine-alpha receptor, plant; D007930:Leucine