Charaterization of Leishmania major Friedlin telomeric terminus.
Here we have characterized Leishmania major (Friedlin) telomeric terminus (the very end) using recombinants obtained by a vector-adaptor cloning protocol. As in L. donovani, the last nine nucleotides of L. major terminus are 5'-GGTTAGGGT-OH 3', differing from Trypanosoma cruzi and T. brucei terminus 5'GGGTTAGGG-OH 3', thus indicating that these sequences are genus specific. We have also made a comparative analysis between L. major and L. donovani telomere-associated sequences, and described a novel non-repeated telomeric associated sequence common to L. major low molecular weight chromosomal bands.
Chiurillo
Miguel Angel
MA
Laboratorio de Genetica Molecular, Instituto de Biología Experimental, Universidad Central de Venezuela, Caracas, Venezuela.
Ramírez
José Luis
JL
D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D007893:Leishmania donovani / Q000235:genetics; D018320:Leishmania major / Q000235:genetics*; D008969:Molecular Sequence Data; D016615:Telomere / Q000737:chemistry / Q000235:genetics*