Aeromonas hydrophila subsp. dhakensis subsp. nov., isolated from children with diarrhoea in Bangladesh, and extended description of Aeromonas hydrophila subsp. hydrophila (Chester 1901) Stanier 1943 (approved lists 1980).
A group of 10 Aeromonas hydrophila-like strains referred to as group BD-2, which mainly comprised haemolytic and cytotoxic isolates from diarrhoeal children in Bangladesh, was subjected to a polyphasic taxonomic study including fluorescent amplified fragment length polymorphism (FAFLP) and ERIC-PCR fingerprinting, microplate DNA-DNA hybridization and extensive phenotypic characterization. Comparison of FAFLP and ERIC-PCR genotypic profiles indicated that the group BD-2 isolates constituted a separate taxon that was most closely related to A. hydrophila DNA hybridization group (HG) 1. Determination of DNA-DNA hybridization values with type and reference strains of all known Aeromonas taxa revealed that the BD-2 group represented a homogeneous taxon (internal DNA homology, 77-94%) that exhibited 78-92% DNA relatedness with the type strain of A. hydrophila HG1. These results indicated that the BD-2 group belongs genotypically to A. hydrophila HG1, but examination of 152 phenotypic characteristics revealed that the group BD-2 isolates differed from representatives of HG1 in eight biochemical properties. The diagnostic value of at least three of these tests, namely utilization of urocanic acid and L-arabinose and acid production from L-arabinose, has been confirmed outside this study by other workers. Collectively, the genotypic and phenotypic data reported suggest that the BD-2 group represents a subspecies of A. hydrophila, for which the name Aeromonas hydrophila subsp. dhakensis subsp. nov. is proposed. Its type strain is P21T (= LMG 19562T= CCUG 45377T). An extended description of A. hydrophila subsp. hydrophila (Chester 1901) Stanier 1943 (Approved Lists 1980) is given.
Huys
Geert
G
Kämpfer
Peter
P
Albert
M John
MJ
Kühn
Inger
I
Denys
Rik
R
Swings
Jean
J
D016980:Aeromonas hydrophila / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001459:Bangladesh / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis*; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length
D004269:DNA, Bacterial