title: Transcription profile of cells infected with human T-cell leukemia virus type I compared with activated lymphocytes.
abstract: Human T-cell leukemia virus type I (HTLV-I) is the etiologic agent for adult T-cell leukemia and the neurological disorder tropical spastic paraparesis/HTLV-I-associated myelopathy. CD4+ T lymphocytes, the primary hosts for HTLV-I, undergo a series of changes that lead to T-cell activation, immortalization, and transformation. To gain insight into the genetic differences between activated and HTLV-I-infected lymphocytes, we performed Affymetrix GeneChip analysis of activated and HTLV-I-infected cells. Using the Hu6800 GeneChip, we identified approximately 763 genes that had differentially regulated expression in at least three of five HTLV-I cell lines. Classification of these genes into functional groups including cellular receptors, kinases, phosphatases, cytokines, signal proteins, and transcription factors provides insight into genes and pathways that are differentially regulated during HTLV-I transformation.
authors.affiliation: Basic Research Laboratory, Virus Tumor Biology Section, National Cancer Institute, Bethesda, Maryland 20892, USA.
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Pise-Masison
authors.forename: Michael
authors.initials: M
authors.lastname: Radonovich
authors.forename: Renaud
authors.initials: R
authors.lastname: Mahieux
authors.forename: Pramita
authors.initials: P
authors.lastname: Chatterjee
authors.forename: Craig
authors.initials: C
authors.lastname: Whiteford
authors.forename: Janet
authors.initials: J
authors.lastname: Duvall
authors.forename: Claire
authors.initials: C
authors.lastname: Guillerm
authors.forename: Antoine
authors.initials: A
authors.lastname: Gessain
authors.forename: John N
authors.initials: JN
authors.lastname: Brady
mesh_terms: D017209:Apoptosis / Q000235:genetics; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics; D002472:Cell Transformation, Viral / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015967:Gene Expression Regulation, Viral; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit; D019169:Jurkat Cells / Q000378:metabolism / Q000502:physiology; D008213:Lymphocyte Activation / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000502:physiology* / Q000821:virology*; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D018797:Cell Cycle Proteins; D016207:Cytokines; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D014157:Transcription Factors