Mutations in the N-terminal Transcriptional Activation Region of Androgen Receptor in the Chinese.
Activation function (AF1) region of N-terminal domain of androgen receptor (AR) is necessary for the transcriptional activation function of AR. The sequences of AF1 in twenty normal chinese were amplified by PCR and determined by direct dsDNA cycle sequencing. Based on this, the mutations in AF1 of AR in patients with androgen insensitivity syndrome(AIS) or prostate cancer(PC) were screened by a combination of single strand conformation polymorphism(SSCP) analysis and direct dsDNA cycle sequencing. The sequences between normal chinese and other races were identical and one point mutation (C966A, Ser296Arg), which had not been reported so far, was identified in a patient with prostate cancer with poor differentiation. That suggests alteration of structure and function in AF1 of N-terminal domain of AR might be relative with PC progression of some patients.
Yu
Xiao-Ling
XL
Department of Pathophysiology, Second Military Medical University, Shanghai 200433, China. Lujian@smmu.edu.cn
Wang
Xiao-Hui
XH
Chen
Guang-Chun
GC
Zhao
Zi-Wen
ZW
Lu
Jian
J