Cytokine network of eutopic and ectopic endometrium in women with adenomyosis.
OBJECTIVE
Recent studies showed the impairment of local cytokine balance in women with external endometriosis, but similar findings concerning direct production of cytokines by immunocompetent cells of women with adenomyosis are absent. In this context, investigation of the cytokine synthesis by mononuclear cells (MNCs) infiltrating eutopic and ectopic endometrium is of special interest.


METHODS
Concentration of interferon-gamma (IFNgamma), interferon-alpha (IFNalpha), tumour necrosis factor-alpha (TNFalpha), interleukin- 1beta (IL-1beta) and epidermal growth factor (EGF) in supernatants (SNs) of 24-hr cultures of MNCs obtained from eutopic and ectopic endometrium of women with adenomyosis was determined by enzyme-linked immunosorbent assay.


RESULTS
The levels of IFNgamma, IFNalpha, TNFalpha, IL-1beta and EGF in SNs of eutopic endometrial MNCs of women with adenomyosis were significantly increased and the content of IL-8 in SNs was reduced compared with that of the control figures. Ectopic MNCs of women with adenomyosis produced higher levels of IFNgamma, IFNalpha and TNFalpha than the MNCs of normal endometrium. The production of IL-1beta, IL-8 and EGF by ectopic endometrial MNCs was significantly reduced.


CONCLUSIONS
The results obtained indicate a significant role of local cytokine production impairment in the development of adenomyosis.
Sotnikova
Natalia
N
Laboratory of Clinical Immunology, Research Institute of Maternity and Childhood, Ivanovo, Russia. sotnikova@imail.ru
Antsiferova
Ioulia
I
Malyshkina
Anna
A
D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism*; D004715:Endometriosis / Q000276:immunology* / Q000473:pathology; D004717:Endometrium / Q000276:immunology* / Q000473:pathology; D004815:Epidermal Growth Factor / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D016898:Interferon-alpha; D007375:Interleukin-1; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D004815:Epidermal Growth Factor; D007371:Interferon-gamma