Validation of the polymerase chain reaction for the diagnosis of human cutaneous leishmaniasis in north-west Colombia.
Leishmania species of the subgenus Viannia account for 88% of all cases of leishmaniasis recorded in Colombia. Correct diagnosis is essential as infection with members of this subgenus can produce disfiguring destruction of the mucosa. Several methods are available to diagnose leishmaniasis in clinical samples. More recently, the polymerase chain reaction (PCR) has been used, with varying sensitivities and specificities depending on the primers used. In this paper we report on the sensitivity and specificity of PCR primers B1/B2 used on clinical samples and compare their use to the conventional parasitological methods. PCR alone is more sensitive than any single conventional method used, but a combination of conventional methods produced comparable sensitivity. PCR is well suited for use in selected cases and as a test for mucosal leishmaniasis.
Isaza
Diana Maria
DM
Instituto Colombiano de Medicina Tropical Antonio Roldan Betancur, Medellin, Colombia.
Arboleda
Margarita
M
Restrepo
Marcos
M
McCann
Sharon H E
SH
Barker
Douglas C
DC
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002648:Child; D003105:Colombia; D018105:DNA, Kinetoplast / Q000235:genetics; D006801:Humans; D007891:Leishmania / Q000235:genetics; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis*; D008875:Middle Aged; D010274:Parasitology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D018105:DNA, Kinetoplast