The absence of factor V Leiden mutation in Malays with recurrent spontaneous abortions.
OBJECTIVE
The objectives of this study were to investigate the prevalence of factor V Leiden mutation in Malay women with recurrent spontaneous abortion and to clarify the contribution of the factor V Leiden mutation to recurrent miscarriages in these women.


METHODS
A prospective case control study between June 1999 and April 2000.


METHODS
Hospital University Science of Malaysia, Kubang Kerian, Kelantan, and Maternal and Child Health Clinic, Pasir Mas, Kelantan, Malaysia.


METHODS
A total of 46 Malay women with a history of three or more first or second trimester miscarriages were studied. The control group consisted of 46 parous women without obstetric complications.


METHODS
Diagnosis of factor V Leiden mutation was made by examination of factor V Leiden allele product following Mnl I digestion of factor V Leiden alleles amplified by polymerase chain reaction.


RESULTS
None of the 46 women with recurrent spontaneous abortion carried the mutation. Also, we found no subject carrying the factor V Leiden alleles in the control group.


CONCLUSIONS
These results suggest that that there is no association between the factor V Leiden mutation and recurrent spontaneous abortion in the Malay population.
Yusoff
Narazah Mohd
NM
Department of Haematology, School of Medical Sciences, University Science of Malaysia, Kelantan.
Abdullah
Wan Zaidah
WZ
Ghazali
Selamah
S
Othman
Mohd Shukri
MS
Baba
Abdul Aziz
AA
Abdullah
Norazmi
N
Isa
Mohd Nizam
MN
Chong
Chan Li
CL
D000026:Abortion, Habitual / Q000453:epidemiology / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000483:Alleles; D001483:Base Sequence; D016022:Case-Control Studies; D005165:Factor V / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D015994:Incidence; D008296:Malaysia / Q000453:epidemiology; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D011446:Prospective Studies; D012008:Recurrence; D012016:Reference Values; D012307:Risk Factors
C095381:factor V Leiden; D005165:Factor V