title: Human anti-rhesus D IgG1 antibody produced in transgenic plants.
abstract: Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.
authors.affiliation: Department of Plant Physiology, Molecular Biology Institute, Copenhagen University, Denmark.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bouquin
authors.forename: Mads
authors.initials: M
authors.lastname: Thomsen
authors.forename: Leif Kofoed
authors.initials: LK
authors.lastname: Nielsen
authors.forename: Trine Hefsgaard
authors.initials: TH
authors.lastname: Green
authors.forename: John
authors.initials: J
authors.lastname: Mundy
authors.forename: Morten Hanefeld
authors.initials: MH
authors.lastname: Dziegiel
mesh_terms: D000372:Agglutination Tests; D017360:Arabidopsis / Q000235:genetics*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics* / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D016897:Respiratory Burst; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D011994:Recombinant Proteins; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen