[Immunogenic and protective characteristics of recombinant Lassa virus NP protein].
Recombinant fragment of Lassa virus (strain Josiah) nucleocapsid protein (corresponding to amino acid residues 141 + 569) constructed by Dr. Jan ter Meulen (Tropenmedizine, Hamburg) was used for immunizing CBA/calac mice. The preparation was injected intraperitoneally twice with 2-week interval in a dose of 10 micrograms. The parameters of the resultant specific humoral and cell-mediated immunity were comparable to those in reference animals immunized with inactivated Lassa virus. Challenge with Lassa virus (10,000 PFU) resulted in 100% death of the reference animals, while of 15 animals immunized with the recombinant NP protein 8 survived.
Ignat'ev
G M
GM
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D007115:Immunization Schedule; D007124:Immunoenzyme Techniques; D007274:Injections, Intraperitoneal; D007835:Lassa Fever / Q000276:immunology / Q000517:prevention & control*; D007836:Lassa virus / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D019590:Nucleocapsid Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013154:Spleen / Q000276:immunology*
D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins
Immunogennye i protektivnye svoĭstva rekombinantnogo belka NP virusa Lassa.