title: Hypoxia influences generation and propagation of electrical activity in embryonic cardiomyocyte clusters.
abstract: The influence of tissue hypoxia on the generation and propagation of excitation was studied in spontaneously beating embryonic cardiomyocyte clusters grown in eight 9-12 days old embryoid bodies. Within the embryoid bodies one to three separately active clusters of cardiomyocytes were found, each having its own pacemaker cell. Lowering of tissue PO(2) caused bradycardia as well as arrhythmia in all embryoid bodies investigated. The mean frequency of the extracellularly recorded action potentials decreased under conditions of pronounced hypoxia from a mean of 1.4-1.8 Hz to below 0.8 Hz. In three embryoid bodies hypoxia-sensitive as well as hypoxia-tolerant cardiomyocyte clusters were found. The hypoxia-insensitive cardiomyocytes showed a low frequency of spontaneous activity. In addition to the observed changes in the generation of excitation, tissue hypoxia caused an approximately 60% reduction in the velocity of conduction within the cardiomyocyte clusters. Moreover, in at least one of the eight experiments propagation failure with an incomplete block in spread of excitation was observed. All hypoxia-induced effects on generation and propagation of embryonic cardiomyocyte excitation were completely reversible after reoxygenation.
authors.affiliation: Department of Physiology I, University of Bonn, Nussallee 11, 53115 Bonn, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Wenzel
authors.forename: M
authors.initials: M
authors.lastname: Dittrich
authors.forename: J
authors.initials: J
authors.lastname: Hescheler
authors.forename: J
authors.initials: J
authors.lastname: Grote
mesh_terms: D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D004553:Electric Conductivity; D004594:Electrophysiology; D006321:Heart / Q000196:embryology*; D051379:Mice; D009206:Myocardium / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis / Q000378:metabolism; D013997:Time Factors
chemical_list: D010100:Oxygen