title: Elevated IL-1beta contributes to antibody suppression produced by stress.
abstract: Acute stressor exposure can facilitate innate immunity and suppress acquired immunity. The present study further characterized the potentiating effect of stress on innate immunity, interleukin-1beta (IL-1beta), and demonstrated that stress-induced potentiation of innate immunity may contribute to the stress-induced suppression of acquired immunity. The long-term effect of stress on IL-1beta was measured by using an ex vivo approach. Sprague-Dawley rats were challenged with lipopolysaccharide (LPS) in vivo, and the IL-1beta response was measured in vitro. Splenocytes, mesenteric lymphocytes, and peritoneal cavity cells had a dose- and time-dependent ex vivo IL-1beta response to LPS. Rats that were exposed to inescapable shock (IS, 100 1.6 mA, 5-s tail shocks, 60-s intertrial interval) and challenged with a submaximal dose of LPS 4 days later had elevated IL-1beta measured ex vivo. To test whether the acute stress-induced elevation in IL-1beta contributes to the long-term suppression in acquired immunity, IL-1beta receptors were blocked for 24 h after stress. Serum anti-keyhole limpet hemocyanin (KLH) immunoglobulin (Ig) was measured. In addition, the acute elevation (2 h post-IS) of splenic IL-1beta in the absence of antigen was verified. Interleukin-1 receptor antagonist prevented IS-induced suppression in anti-KLH Ig. These data support the hypothesis that stress-induced increases in innate immunity (i.e., IL-1beta) may contribute to stress-induced suppression in acquired immunity (i.e., anti-KLH Ig).
authors.affiliation: Department of Kinesiology and Applied Physiology, University of Colorado at Boulder, 80309, USA.
authors.forename: Albert
authors.initials: A
authors.lastname: Moraska
authors.forename: Jay
authors.initials: J
authors.lastname: Campisi
authors.forename: Kien T
authors.initials: KT
authors.lastname: Nguyen
authors.forename: Steven F
authors.initials: SF
authors.lastname: Maier
authors.forename: Linda R
authors.initials: LR
authors.lastname: Watkins
authors.forename: Monika
authors.initials: M
authors.lastname: Fleshner
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004597:Electroshock; D004797:Enzyme-Linked Immunosorbent Assay; D006433:Hemocyanins / Q000276:immunology; D007111:Immunity, Cellular / Q000187:drug effects; D007074:Immunoglobulin G / Q000096:biosynthesis; D007375:Interleukin-1 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D007375:Interleukin-1; D008070:Lipopolysaccharides; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin