title: [Dystrophin detection by immunofluorescent technique for diagnosing muscular dystrophy].
abstract: OBJECTIVE
  To establish a specific technique for diagnosing and classifying Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD), facioscapulohumeral muscular dystrophy (FSHD) and neurologic dystrophy.
  
  
  METHODS
  Forty-seven cases were detected by immunofluorescence technique for analyzing dystrophin located in skeletal muscle cell membrane with the use of mouse monoclonal antibodies, goat and rabbit polyclonal antibodies.
  
  
  RESULTS
  The normal individuals showed ringed positive staining stripe around muscle fibers. Negative result of staining was seen in 16 DMD patients. Eleven BMD patients had discontinuous or a patchy positive staining pattern, and all of 10 FSHD and 10 neurological amyotrophic patients showed positive dystrophin staining.
  
  
  CONCLUSIONS
  Detecting dystrophin in the skeletal muscle cell membrane of muscular patients is an efficient technique for diagnosing and classifying various types of muscular dystrophy.
authors.affiliation: Department of Neurology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, 510080 P. R. China. czym@gzsums.edu.cn
authors.forename: Xun
authors.initials: X
authors.lastname: Wang
authors.forename: Youmei
authors.initials: Y
authors.lastname: Xie
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhuoling
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D016189:Dystrophin / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000473:pathology; D009136:Muscular Dystrophies / Q000175:diagnosis* / Q000378:metabolism; D020388:Muscular Dystrophy, Duchenne / Q000175:diagnosis / Q000378:metabolism; D020391:Muscular Dystrophy, Facioscapulohumeral / Q000175:diagnosis / Q000378:metabolism; D009468:Neuromuscular Diseases / Q000175:diagnosis / Q000378:metabolism
chemical_list: D016189:Dystrophin