Molecular characterization of bovine CD26 upregulated by a staphylococcal superantigen.
In this report, we describe the cloning, sequencing, and expression of the bovine orthologue of CD26 (BoCD26). Several monoclonal antibodies specific for a molecule, activation molecule 3 (ACT3), aberrantly expressed on superantigen-stimulated bovine CD8(+) lymphocytes, reacted with recombinant BoCD26 expressed in COS-7 and CHO cells. We also showed that human CD8(+) T cells stimulated by a superantigen expressed CD26 at high levels. These results demonstrate that ACT3 is identical to BoCD26 and suggest that CD26 upregulation on CD8(+) T cells is a general phenomenon of superantigens and not limited to their effects on bovine cells.
Lee
Sang-Un
SU
Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID 83844, USA.
Park
Yong-Ho
YH
Davis
William C
WC
Hamilton
Jo
J
Naessens
Jan
J
Bohach
Gregory A
GA
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001483:Base Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016466:CHO Cells; D019556:COS Cells; D002417:Cattle; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006224:Cricetinae; D018819:Dipeptidyl Peptidase 4 / Q000096:biosynthesis* / Q000235:genetics*; D004768:Enterotoxins / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013211:Staphylococcus aureus / Q000276:immunology; D018089:Superantigens / Q000276:immunology*; D015854:Up-Regulation
D000942:Antigens, Bacterial; D004768:Enterotoxins; D018089:Superantigens; C034959:enterotoxin C, staphylococcal; D018819:Dipeptidyl Peptidase 4