Correlation between HSP90 induction kinetics in murine leukemia cells and the amount of cisplatin over a wide range of cytostatic concentrations.
The induction of HSP90 in murine erythroleukemia cells, clone F4 N, by cisplatin (DDP) was examined using indirect immunofluorescence and avidin-biotin technique, and compared with cisplatin cytotoxicity. A reverse dependence of HSP90 induction time was found on a wide range of cisplatin concentrations (0.5-10 microM), which proved to be cytostatic up to 48 h of continuous treatment. Thus, the observed induction pattern of HSP90 in F4 N cells strictly correlated with their high tolerance toward DDP. This indicates that HSP90 might be responsible, at least in part, for cisplatin resistance of F4 N cells.
Detcheva
Roumiana L
RL
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia. roumi@obzor.bio21.bas.bg
Kenderov
Andrey N
AN
Russinova
Angelina I
AI
Spassovska
Nadejda C
NC
Kolev
Kolyo G
KG
Grancharov
Konstantin C
KC
D000818:Animals; D000911:Antibodies, Monoclonal; D002945:Cisplatin / Q000494:pharmacology; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis* / Q000187:drug effects; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D051379:Mice; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D018841:HSP90 Heat-Shock Proteins; D002945:Cisplatin