title: Aberrant nuclear trafficking of La protein leads to disordered processing of associated precursor tRNAs.
abstract: Eukaryotic precursor tRNAs undergo extensive processing prior to nuclear export. The first of multiple factors to interact with pre-tRNAs and other nascent transcripts is the La protein. Using suppressor and wild-type tRNAs, we demonstrate that the normal distribution of cellular end-processed and spliced tRNA species is disordered by La proteins that lack a conserved nuclear retention element. Fission yeast or human La mutants that lack this element enter nuclei and stabilize nascent pre-tRNA but are aberrantly exported and fail to support normal tRNA processing. Instead, anomalous 5' and 3' end-containing, spliced tRNAs accumulate, complexed with the mutant La protein. Thus, appropriate nuclear trafficking by La affects the normal order of pre-tRNA processing.
authors.affiliation: Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.
authors.forename: Robert V
authors.initials: RV
authors.lastname: Intine
authors.forename: Miroslav
authors.initials: M
authors.lastname: Dundr
authors.forename: Tom
authors.initials: T
authors.lastname: Misteli
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Maraia
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens / Q000737:chemistry / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000378:metabolism*; D022821:RNA Splice Sites; D012326:RNA Splicing; D012261:Ribonucleoproteins / Q000737:chemistry / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics; D014162:Transfection; D000097585:SS-B Antigen
chemical_list: D001324:Autoantigens; D012322:RNA Precursors; D022821:RNA Splice Sites; D012261:Ribonucleoproteins; D000097585:SS-B Antigen