WARP is a new member of the von Willebrand factor A-domain superfamily of extracellular matrix proteins.
We report a new member of the von Willebrand factor A-domain protein superfamily, WARP (for von Willebrand factor A-domain-related protein). The full-length mouse WARP cDNA is 2.3 kb in size and predicts a protein of 415 amino acids which contains a signal sequence, a VA-like domain, two fibronectin type III-like repeats, and a short proline- and arginine-rich segment. WARP mRNA was expressed predominantly in chondrocytes and in vitro expression experiments in transfected 293 cells indicated that WARP is a secreted glycoprotein that forms disulphide-bonded oligomers. We conclude that WARP is a new member of the von Willebrand factor A-domain (VA-domain) superfamily of extracellular matrix proteins which may play a role in cartilage structure and function.
Fitzgerald
Jamie
J
Cell and Matrix Biology Research Unit, Murdoch Childrens Research Institute and Department of Paediatrics, University of Melbourne, Royal Children's Hospital, Parkville, Vic., Australia. fitzgerj@cryptic.rch.unimelb.edu.au
Tay Ting
Su
S
Bateman
John F
JF
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000302:isolation & purification; D005347:Fibroblasts / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014841:von Willebrand Factor / Q000737:chemistry* / Q000378:metabolism
D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D012333:RNA, Messenger; C460760:VWA1 protein, human; C460761:WARP protein, mouse; D014841:von Willebrand Factor