title: A novel striated tropomyosin incorporated into organized myofibrils of cardiomyocytes in cell and organ culture.
abstract: Striated muscle tropomyosin is classically described as consisting of 10 exons, 1a, 2b, 3, 4, 5, 6b, 7, 8, and 9a/b, in both skeletal and cardiac muscle. A novel isoform found in embryonic axolotl heart maintains exon 9a/b of striated muscle but also has a smooth muscle exon 2a instead of exon 2b. Translation and subsequent incorporation into organized myofibrils, with both isoforms, was demonstrated with green fluorescent protein fusion protein construct. Mutant axolotl hearts lack sufficient tropomyosin in the ventricle and this smooth/striated chimeric tropomyosin was sufficient to replace the missing tropomyosin and form organized myofibrils.
authors.affiliation: Department of Cell and Developmental Biology, Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Zajdel
authors.forename: Jean M
authors.initials: JM
authors.lastname: Sanger
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Denz
authors.forename: Sung
authors.initials: S
authors.lastname: Lee
authors.forename: Syamalima
authors.initials: S
authors.lastname: Dube
authors.forename: Bernard J
authors.initials: BJ
authors.lastname: Poiesz
authors.forename: Joseph W
authors.initials: JW
authors.lastname: Sanger
authors.forename: Dipak K
authors.initials: DK
authors.lastname: Dube
mesh_terms: D000557:Ambystoma; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D005260:Female; D049452:Green Fluorescent Proteins; D006321:Heart / Q000196:embryology; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D018482:Muscle, Skeletal / Q000378:metabolism*; D009154:Mutation; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism*; D009924:Organ Culture Techniques; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014335:Tropomyosin / Q000235:genetics / Q000378:metabolism*
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D014335:Tropomyosin; D049452:Green Fluorescent Proteins