Determination of fenoterol in human plasma by HPLC with fluorescence detection after derivatization.
A new method for the determination of fenoterol is described, which uses HPLC separation with fluorescence detection. Dobutamine is employed as an internal standard. The separation was achieved on a short reversed phase column with a mobile phase consisting of water, acetonitrile and methanol. Prior to chromatography both analytes are derivatized with 9-chloroformyl-carbazole. Isolation of the analytes from plasma is carried out by liquid-liquid extraction into 2-butanol after protein precipitation with acetonitrile. The method is capable of estimating fenoterol concentrations in the sub-nanogram per ml range with sufficient accuracy and precision. The determination of fenoterol can now be carried out in the average laboratory without radiolabelled material.
Meineke
Ingolf
I
Department of Clinical Pharmacology, University of Göttingen, Robert-Koch-Strasse 40, D-37075 Gottingen, Germany. imeineke@med.uni-goettingen.de
Steinmetz
Hannelore
H
Kramer
Skaidrit
S
Gleiter
Christoph H
CH
D000318:Adrenergic beta-Agonists / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005280:Fenoterol / Q000097:blood*; D006801:Humans; D011247:Pregnancy; D015203:Reproducibility of Results
D000318:Adrenergic beta-Agonists; D005280:Fenoterol