The sensory nitrergic nature of the hepatic insulin sensitizing substance mechanism in conscious rabbits.
Functional deterioration of sensory fibres in the anterior hepatic plexus or intraportal administration of 7-nitro indazole (1 mg/kg), a selective inhibitor of neural nitric oxide (NO) synthase, caused insulin resistance as determined by hyperinsulinaemic (100 micro U/ml) euglycaemic (5.5 mmol/l) glucose clamping in chronically instrumented conscious rabbits. Intraportal nitroglycerin restored insulin sensitivity in either case. We conclude that NO of sensory neural origin plays a major role in endogenous neurogenic insulin sensitizing mechanisms.
Porszasz
Robert
R
Department of Pharmacology and Pharmacotherapy, Medical University of Debrecen, H-4032, Nagyerdei krt 98, Debrecen 4032, Hungary.
Legvari
Gyorgyi
G
Nemeth
Jozsef
J
Literati
Peter N
PN
Szolcsanyi
Janos
J
Szilvassy
Zoltan
Z
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology; D066298:In Vitro Techniques; D007191:Indazoles / Q000494:pharmacology; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology*; D008099:Liver / Q000294:innervation / Q000378:metabolism*; D008297:Male; D009412:Nerve Fibers / Q000502:physiology; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D011817:Rabbits; D011863:Radioimmunoassay
D001786:Blood Glucose; D007191:Indazoles; D007328:Insulin; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D002211:Capsaicin; C080122:7-nitroindazole