title: Dynamic imaging of T cell-dendritic cell interactions in lymph nodes.
abstract: T cell immune responses begin within organized lymphoid tissues. The pace, topology, and outcomes of the cellular interactions that underlie these responses have, so far, been inferred from static imaging of sectioned tissue or from studies of cultured cells. Here we report dynamic visualization of antigen-specific T cells interacting with dendritic cells within intact explanted lymph nodes. We observed immunological synapse formation and prolonged interactions between these two cell types, followed by the activation, dissociation, and rapid migration of T cells away from the antigenic stimulus. This high-resolution spatiotemporal analysis provides insight into the nature of cell interactions critical to early immune responses within lymphoid structures.
authors.affiliation: Lymphocyte Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Sabine
authors.initials: S
authors.lastname: Stoll
authors.forename: Jérôme
authors.initials: J
authors.lastname: Delon
authors.forename: Tilmann M
authors.initials: TM
authors.lastname: Brotz
authors.forename: Ronald N
authors.initials: RN
authors.lastname: Germain
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D000941:Antigens / Q000276:immunology; D015703:Antigens, CD; D002450:Cell Communication; D002455:Cell Division; D002465:Cell Movement; D003713:Dendritic Cells / Q000276:immunology* / Q000502:physiology / Q000648:ultrastructure; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted; D007365:Intercellular Junctions / Q000502:physiology / Q000648:ultrastructure; D051917:Leukosialin; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012795:Sialoglycoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology / Q000648:ultrastructure
chemical_list: D000941:Antigens; D015703:Antigens, CD; D005456:Fluorescent Dyes; D051917:Leukosialin; D011993:Recombinant Fusion Proteins; D012795:Sialoglycoproteins; C497863:Spn protein, mouse