Preparation of well-defined bovine polyhemoglobin based on dimethyl adipimidate and glutaraldebyde cross-linkage.
A well-defined bovine polyhemoglobin was prepared by dimethyl adipimidate (DMA) and glutaraldehyde double cross-linkage method. DMA was used to block some amino groups of hemoglobin, followed by further polymerization with glutaraldehyde. The amino modification degree of hemoglobin was 32% when DMA reacted with hemoglobin at the molar ratio of 200. The bovine polyhemoglobin with narrow molecular weight distribution (mainly 128 kDa) was obtained when glutaraldehyde reacted with DMA-modified hemoglobin. The P(50) and the Hill coefficient for DMA-modified hemoglobin were 19.4 mm Hg and 2.28, respectively, while those for the bovine polyhemoglobin were 15.1 mm Hg and 1.70, respectively. The number of Bohr protons released for DMA-modified hemoglobin and the polyhemoglobin was 0.86 and 0.56 H/tetramer, respectively.
Hu
Tao
T
National Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, PR China. hutao1975@yahoo.com
Su
Zhiguo
Z
D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004119:Dimethyl Adipimidate / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005976:Glutaral / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000378:metabolism
D001802:Blood Substitutes; D003432:Cross-Linking Reagents; D006454:Hemoglobins; C037158:polyhemoglobin; D004119:Dimethyl Adipimidate; D010100:Oxygen; D005976:Glutaral