title: Successive cultures of mature hepatocytes for hepatocyte autotransplantation to assist liver function after liver resection for cancer.
abstract: We developed a method for the rapid successive cultures of adult rat mature hepatocytes on plastic dishes while avoiding viral transformation or co-culture with other cell lines. This method also allows for culturing adult human mature hepatocytes up to the secondary culture. These can be expected to provide a good source for hepatocyte autotransplantation, and, combined with the previously reported methods for the transplantation of hepatocytes into the spleen, a promising option for the support of liver function after liver resection for cancer without the need for immunosuppressive agents.
authors.affiliation: Department of Surgery, Shiga Medical Center for Adults, Moriyama City, Shiga 524, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Funaki
authors.forename: Junji
authors.initials: J
authors.lastname: Tanaka
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Gakuji
authors.initials: G
authors.lastname: Ohshio
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nonaka
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Yotsumoto
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Sugie
authors.forename: Masayuki
authors.initials: M
authors.lastname: Imamura
mesh_terms: D000328:Adult; D000418:Albumins / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D017690:Cell Transplantation; D002478:Cells, Cultured / Q000166:cytology; D006003:Glycogen / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology* / Q000637:transplantation*; D006801:Humans; D007166:Immunosuppressive Agents; D008099:Liver / Q000502:physiology* / Q000601:surgery; D008113:Liver Neoplasms / Q000601:surgery*; D008297:Male; D008858:Microscopy, Phase-Contrast; D008938:Mitosis; D051381:Rats; D017208:Rats, Wistar; D014182:Transplantation, Autologous
chemical_list: D000418:Albumins; D007166:Immunosuppressive Agents; D006003:Glycogen