[Human immunodeficiency virus (HIV) co-receptors expression in mononuclear leucocytes (ML) of HIV+ patients].
Variations of the expression of CXCR4 and CCR5 HIV co-receptors after non stimulated culture of peripheral blood mononuclear cells (PBMC) from HIV+ patients were studied. Expression of CCR5 on both CD4+ and CD8+ T lymphocytes was reduced after 7 days and remained low throughout the culture. CXCR4 levels remained stable in both lymphocyte subpopulations. No significant changes were observed in control HIV- PBMC cultures. In order to ascertain if the CCR5 changes were associated to in vitro HIV replication, 6 days pre-cultured HIV- PBMC were infected with HIV+ culture supernatants. After 3 days CCR5 expression was reduced both in CD4+ and in CD8+ T lymphocytes, while CXCR4 expression was not, coincident with initiation of HIV replication in culture. These results suggest that CCR5 down modulation in CD4+ or CD8+ T lymphocytes is a consequence of HIV replication.
Belmonte
L
L
Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires. lilibelm@hematologia.anm.edu.ar
de Elizalde de Bracco
M M
MM
Ruibal-Ares
B
B
D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism* / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism* / Q000821:virology; D006678:HIV / Q000276:immunology / Q000378:metabolism*; D015658:HIV Infections / Q000276:immunology / Q000378:metabolism / Q000821:virology; D006801:Humans; D019713:Receptors, CCR5 / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000378:metabolism*; D014779:Virus Replication
D019713:Receptors, CCR5; D019718:Receptors, CXCR4
Expresion de co-receptores para el virus de la inmunodeficiencia humana (VIH) luego del cultivo de leucocitos mononucleares (LM) de pacientes VIH+.