title: GDH1 expression is regulated by GLN3, GCN4, and HAP4 under respiratory growth.
abstract: In the yeast Saccharomyces cerevisiae, two NADP(+)-dependent glutamate dehydrogenase isoenzymes encoded by GDH1 and GDH3 catalyze the synthesis of glutamate from ammonium and alpha-ketoglutarate. In this work we analyzed GDH1 transcriptional regulation, in order to deepen the studies in regard to its physiological role. Our results indicate that: (i) GDH1 expression is strictly controlled in ethanol-grown cultures, constituting a fine-tuning mechanism that modulates the abundance of Gdh1p monomers under this condition, (ii) GDH1 expression is controlled by transcriptional activators that have been considered as exclusive of either nitrogen (Gln3p and Gcn4p) or carbon metabolism (HAP complex), and (iii) chromatin remodeling complexes play a role in GDH1 expression; ADA2 and ADA3 up-regulated GDH1 expression on ethanol, while that on glucose was ADA3-dependent. SPT3 and SNF2 activated GDH1 expression on either carbon source whereas GCN5 played no role in any condition tested. The above described combinatorial control results in a refined mechanism that coordinates carbon and nitrogen utilization.
authors.affiliation: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, 04510 Mexico City, México.
authors.forename: Lina
authors.initials: L
authors.lastname: Riego
authors.forename: Amaranta
authors.initials: A
authors.lastname: Avendaño
authors.forename: Alexander
authors.initials: A
authors.lastname: DeLuna
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Rodríguez
authors.forename: Alicia
authors.initials: A
authors.lastname: González
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D023081:CCAAT-Binding Factor / Q000378:metabolism*; D002455:Cell Division; D004268:DNA-Binding Proteins / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D051548:Histone Acetyltransferases; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation; D000099224:p300-CBP-Associated Factor
chemical_list: D023081:CCAAT-Binding Factor; D004268:DNA-Binding Proteins; D000431:Ethanol; D005656:Fungal Proteins; D005969:Glutamate Dehydrogenase; D051548:Histone Acetyltransferases; D011494:Protein Kinases; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C071664:GLN3 protein, S cerevisiae; C061437:HAP4 protein, S cerevisiae; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor