Effects of vitamin K(2) on bone of ovariectomized rats and on a rat osteoblastic cell line.
The effects of vitamin K(2) on bone mineral density (BMD) and bone metabolic markers of ovariectomized rats, and those on mRNA expression of osteocalcin and IL-6 on a rat osteoblastic cell line, were investigated. BMD and bone metabolic markers were examined in ovariectomized rats after 2 months' treatment with vitamin K(2), and mRNA expression of osteocalcin and IL-6 were measured in the cell line after 24-hour treatment with vitamin K(2). Vitamin K(2) attenuated the decline in BMD after ovariectomy in the rats, and suppressed serum deoxypyridinoline levels of the ovariectomized rats. No effect on osteocalcin and IL-6 mRNA expression on the cell line was observed. In conclusion, vitamin K(2) has a bone-protective effect on ovariectomized rats.
Iwamoto
Ichiro
I
Department of Obstetrics and Gynecology, Kagoshima University, Sakuragaoka, Kagoshima, Japan. ichiro@m.kufm.kagoshima-uc.ac.jp
Kosha
Shoichiro
S
Fujino
Toshinori
T
Nagata
Yukihiro
Y
D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects*; D002460:Cell Line; D005260:Female; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis / Q000235:genetics; D010052:Ovariectomy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D024482:Vitamin K 2 / Q000494:pharmacology*
D015850:Interleukin-6; D012333:RNA, Messenger; D015675:Osteocalcin; D024482:Vitamin K 2