Electroosmotic flow controllable coating on a capillary surface by a sol-gel process for capillary electrophoresis.
A simple coating procedure employing a sol-gel process to modify the inner surface of a bare fused-silica capillary with a positively charged quaternary ammonium group is established. Scanning electron microscopic studies reveal that a smooth coating with 1 to approximately 2 microm thickness can be obtained at optimized coating conditions. With 40 mM citrate as a running electrolyte, the plot of electroosmotic flow (EOF) versus pH shows a unique three-stage EOF pattern from negative to zero and then to positive over a pH range of 2.5 to 7.0. At pH above 5.5, the direction of the EOF is from the anode to the cathode, as is the case in a bare fused-silica capillary, and the electroosmotic mobility increases as the pH increases. However, the direction of the EOF is reversed at pH below 4.0. Over the pH range of 4.0 to 5.5, zero electroosmotic mobility is obtained. Such a three-stage EOF pattern has been used to separate six aromatic acids under suppressed EOF and to separate nitrate and nitrite with the anions migrating in the same direction as the EOF. The positively charged quaternary ammonium group on the coating was also utilized to minimize the adsorption problem during the separation of five basic drugs under suppressed EOF and during the separation of four basic proteins with the cations migrate in the opposite direction as the EOF. Also, the stability and reproducibility of this column are good.
Hsieh
Yi-Yun
YY
Department of Chemistry, National Chung Cheng University, Ming-Hsiung, Chia-Yi, Taiwan.
Lin
Ya-Hsien
YH
Yang
Jr-Shiung
JS
Wei
Guor-Tzo
GT
Tien
Pei
P
Chau
Lai-Kwan
LK
D019075:Electrophoresis, Capillary / Q000379:methods*; D005782:Gels; D008855:Microscopy, Electron, Scanning; D009995:Osmosis; D013499:Surface Properties
D005782:Gels