Automated acquisition of aptamer sequences.
While the in vitro selection of nucleic acid binding species (aptamers) requires numerous liquid-handling steps, these steps are relatively straightforward and the overall process is therefore amenable to automation. Here we demonstrate that automated selection techniques are capable of generating aptamers against a number of diverse protein targets. Automated selection techniques can be integrated with automated analytical methods, including sequencing, determination of binding constants, and structural analysis. The methods that have so far been developed can be further multiplexed, and it should soon be possible to attempt the selection of aptamers against organismal proteomes or metabolomes.
Cox
J Colin
JC
Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.
Rajendran
Manjula
M
Riedel
Timothy
T
Davidson
Eric A
EA
Sooter
Letha J
LJ
Bayer
Travis S
TS
Schmitz-Brown
Mary
M
Ellington
Andrew D
AD
D001331:Automation; D001483:Base Sequence; D020650:Combinatorial Chemistry Techniques; D008024:Ligands; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
D008024:Ligands; D009838:Oligodeoxyribonucleotides