Assessment of chromosomal losses and gains in hepatocellular carcinoma.
We examined the chromosomal changes of 22 hepatocellular carcinomas (HCCs) by comparative genomic hybridization (CGH) analysis and compared the results with that of allelotype by polymerase chain reaction based loss of heterozygosity (PCR-LOH) analysis. By CGH analysis, frequent chromosomal losses were noted in the chromosomal region of 4q (59%), 8p (77%), and 16q (50%), whereas gains were noted in 1q (86%) and 8q (77%). All of these chromosomal arms were revealed to have frequent allelic imbalances by PCR-LOH analysis, however, 9% of chromosomal aberrations were detected only by CGH analysis and 2% were detected only by PCR-LOH analysis. Our results suggest that CGH analysis gives more precise results for the screening of chromosomal aberrations in HCCs than that of PCR-LOH analysis with randomly selected microsatellite markers.
Chang
Joon
J
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea.
Kim
Nam-Gyun
NG
Piao
Zhe
Z
Park
Chanil
C
Park
Kang-Sik
KS
Paik
Young-Ki
YK
Lee
Woo-Jung
WJ
Kim
Byong Ro
BR
Kim
Hoguen
H
D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D020440:Gene Duplication; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity; D016133:Polymerase Chain Reaction / Q000379:methods