Encapsulation of insulin in chitosan-coated alginate beads: oral therapeutic peptide delivery.
Insulin was encapsulated in calcium alginate beads coated with chitosan. Its release from alginate-chitosan and alginate-chitosan-glutaraldehyde beads was studied in artificial gastric (pH 1.2) and intestinal (pH 7.5) fluids. By comparing the release amounts, the ionic interaction between alginate-chitosan matrix with the medium pH's, intestinal fluid was found to be the better. The degradation of released insulin was also searched, even after 6 h incubation, the beads remained stable and the undegraded insulin seemed to be sufficient for the physiological conditions. Consequently, it can be said that the system can be offered for oral delivery of the therapeutic peptide drug insulin.
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Biochemistry Department, Faculty of Science, Ege University, Bornova-Izmir, Turkey. tatar@sci.ege.edu.tr
Zihnioğlu
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D000284:Administration, Oral; D000464:Alginates; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D003692:Delayed-Action Preparations; D003920:Diabetes Mellitus / Q000188:drug therapy; D004337:Drug Carriers; D016503:Drug Delivery Systems; D020723:Glucuronic Acid; D005976:Glutaral; D006603:Hexuronic Acids; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007328:Insulin / Q000008:administration & dosage* / Q000493:pharmacokinetics
D000464:Alginates; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006603:Hexuronic Acids; D007328:Insulin; D002686:Chitin; D020723:Glucuronic Acid; D048271:Chitosan; D005976:Glutaral