Bisucaberin--a dihydroxamate siderophore isolated from Vibrio salmonicida, an important pathogen of farmed Atlantic salmon (Salmo salar).
A siderophore of the bacterial fish pathogen, Vibrio salmonicida, was isolated from low-iron culture supernatant and structurally characterized as bisucaberin by FTICR- and FAB-MS, NMR and GC-MS analysis of the hydrolysis products. Although the cyclic dihydroxamate bisucaberin has previously been isolated from a marine bacterium, Alteromonas haloplanktis, its involvement in cold-water vibriosis of Atlantic salmon (Salmon salar) is novel. Bisucaberin production in iron-limited media was highest at temperatures around and below 10 degrees C, correlating well with temperatures at which outbreaks of cold-water vibriosis occur. Due to the very high stability constant of K = 32.2, bisucaberin is a most efficient iron scavenger which may contribute to the virulence of V. salmonicida in Atlantic salmon.
Winkelmann
Gunther
G
Institut für Mikrobiologie und Biotechnologie, Universität Tübingen, Germany. winkelmann@uni.tuebingen.de
Schmid
Dietmar G
DG
Nicholson
Graeme
G
Jung
Günther
G
Colquhoun
Duncan J
DJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005393:Fish Diseases / Q000382:microbiology; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D007501:Iron / Q000378:metabolism; D013058:Mass Spectrometry; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D020317:Salmo salar / Q000382:microbiology*; D017262:Siderophores / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D014733:Vibrio / Q000737:chemistry* / Q000472:pathogenicity; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary; D014774:Virulence
D010456:Peptides, Cyclic; D017262:Siderophores; C054842:bisucaberin; D007501:Iron