Betaig-h3 supports keratinocyte adhesion, migration, and proliferation through alpha3beta1 integrin.
betaig-h3 is an extracellular matrix protein and its expression is highly induced by TGF-beta and it has also been suggested to play important roles in skin wound healing. In this paper, we demonstrate that betaig-h3 is present in the papillary layer of dermis and synthesized in the basal keratinocytes in vivo and its expression is induced by TGF-beta in normal human keratinocytes (NHEK) and HaCaT cells. betaig-h3 mediates not only adhesion and spreading of keratinocytes but also supports migration and proliferation. These activities are mediated through interacting with alpha3beta1 integrin. Previously identified two alpha3beta1 integrin-interacting motifs of betaig-h3, EPDIM, and NKDIL, are responsible for these activities. The results suggest that betaig-h3 may regulate keratinocyte functions in normal skin and potentially during wound-healing process.
Bae
Jong-Sup
JS
Department of Biochemistry, Kyungpook National University School of Medicine, Taegu 700-422, Republic of Korea.
Lee
Suk-Hee
SH
Kim
Jung-Eun
JE
Choi
Je-Yong
JY
Park
Rang-Woon
RW
Yong Park
Jae
J
Park
Hyun-Sook
HS
Sohn
Young-Sook
YS
Lee
Dong-Sin
DS
Bae Lee
Eunhee
E
Kim
In-San
IS
D000595:Amino Acid Sequence; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002465:Cell Movement; D002478:Cells, Cultured; D017124:Conserved Sequence; D020405:Dermis / Q000033:anatomy & histology / Q000737:chemistry; D016326:Extracellular Matrix Proteins; D006801:Humans; D039201:Integrin alpha3beta1; D016023:Integrins / Q000502:physiology*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D000099222:betaIG-H3 Protein
D016326:Extracellular Matrix Proteins; D039201:Integrin alpha3beta1; D016023:Integrins; D009363:Neoplasm Proteins; D010455:Peptides; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein