Monoclonal antibody of IgG isotype against a cross-reactive lipopolysaccharide epitope of Chlamydia and Salmonella Re chemotype enhances infectivity in L-929 fibroblast cells.
A murine monoclonal antibody (MAb) 202D7 of IgG3 isotype recognizes a lipopolysaccharide (LPS) epitope of Chlamydia spp. and cross-reacts with the Re chemotype LPS of Salmonella and Escherichia coli. The antibody exhibits strong complement activating properties and stimulates phagocytosis of Salmonella enterica serovar Minnesota Re mutant by murine macrophages. Salmonella Re mutants are non-invasive for cell monolayers but still can enter and replicate in L-929 murine fibroblast cells. The entry of bacteria within the cells increases five-fold in the presence of MAb 202D7. The antibody mediates attachment and enhances five-fold the infectivity of Chlamydia pneumoniae into L-929 cells, which suggests a possible IgG-mediated mechanism of entry and survival of the pathogen in fibroblast cells.
Haralambieva
Iana H
IH
Department of Microbiology, Medical University of Sofia, Zdrave 2 street, 1431, Sofia, Bulgaria.
Iankov
Ianko D
ID
Petrov
Dragomir P
DP
Mladenov
Ivan V
IV
Mitov
Ivan G
IG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D019067:Antibody-Dependent Enhancement; D002460:Cell Line; D016993:Chlamydophila pneumoniae / Q000276:immunology / Q000472:pathogenicity*; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D005347:Fibroblasts / Q000382:microbiology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D019779:Salmonella enterica / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D008070:Lipopolysaccharides