title: [Assessment of the basal membrane status as prognostic factor in renal carcinoma].
abstract: OBJECTIVE
  To know the basal membrane (BM) integrity in renal cell carcinoma (RC) and its importance as prognostic factor.
  
  
  METHODS
  73 patients with RC were selected. Immunohistochemistry with monoclonal antibodies against basal proteins laminin and collagen IV was performed. Percentage for BM fragmentation in the whole tumour was considered taking 75% as cut off.
  
  
  RESULTS
  Follow-up was 6.3 +/- 4.3 years and 27 patients progressed. Correlation between laminin and collagen IV was significative (p = 0.000). A BM fragmentation expressed with laminin bigger than 75% was related to tumoural symptoms (p = 0.019), worse grade (p = 0.004) and necrosis in more than 10% of the tumour (p = 0.000). Fragmentation observed with collagen IV was associated to tumours greater than 7 cm (p = 0.014). Those patients whose tumours displayed more than 75% of BM fragmentation, measured with collagen IV, presented worse survival (p = 0.042). A similar trend was observed in the case of laminin, but it did not reach statistic significance (p = 0.119). In the unvariated analysis grade III-IV, more than 10% of necrosis within the tumour, tumoural symptoms and BM fragmentation bigger than 75% measured with collagen IV were prognostic, while only grade and necrosis did so in the multivariate analysis.
  
  
  CONCLUSIONS
  Collagen IV and laminin represent nicely, with a similar expression pattern, the BM fragmentation in RC. Within a battery of immunohistochemical markers to study RC at least one of them should be included because their prognostic implication.
authors.affiliation: Servicio de Urología, Instituto Valenciano de Oncología, Valencia.
authors.forename: J
authors.initials: J
authors.lastname: Rubio Briones
authors.forename: L
authors.initials: L
authors.lastname: Morell Quadreny
authors.forename: J A
authors.initials: JA
authors.lastname: López Guerrero
authors.forename: J
authors.initials: J
authors.lastname: Casanova Ramón-Borja
authors.forename: C
authors.initials: C
authors.lastname: Mejía Vázquez
authors.forename: I
authors.initials: I
authors.lastname: Iborra Juan
authors.forename: A
authors.initials: A
authors.lastname: Pellín Pérez
authors.forename: E
authors.initials: E
authors.lastname: Solsona Narbón
authors.forename: A
authors.initials: A
authors.lastname: Llombart Bosch
mesh_terms: D001485:Basement Membrane / Q000473:pathology*; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis
vernacular_title: Valoración del estado de la membrana basal como factor pronóstico en el carcinoma renal.