title: Growth inhibitory effect of paradicsompaprika in cancer cell lines.
abstract: We investigated whether components of paradicsompaprika have direct antitumor effects or inhibitory effects on cancer growth, using its water extract. We applied collagen gel droplet embedded culture drug sensitivity test (CD-DST) as a screening method, which was developed based on the characteristics of cell culture on collagen matrix. Colon adenocarcinoma cells, epithelial cells of lung cancer, and cervical cancer cells were used. Paradicsompaprika is classified as Capsiucum annume L. var. grossum of Solanaceae. It is the first of the Hungarian species that was planted in Japan. It is available as TOMA-P in Japan. TOMA-P contains abundant carotenoids including capsanthin and beta-carotene. Water extract of paradicsompaprika was added to each cell at each concentration, and the mixture was cultured for 24 h and 7 days. The inhibitory effects against lung cancer and cervical cancer were observed concentration- and time-dependently. The effect was more prominent against lung cancer. The growth of bowel cancer cells was observed after the 7-day exposure of paradicsompaprika at the concentrations below the highest concentration compared to the control. At the highest concentration, the growth inhibition was not different between the 24-h exposure and the 7-day exposure, which suggests that tumor dormancy was induced. Results of the present study suggest that the water extract of paradicsompaprika can be a candidate of a new anticancer agent. Fat soluble component of paradicsompaprika, capsanthin is regarded as an anti-promoter of cancer. Thus, paradicsompaprika possesses chemopreventive and inhibitory effects on cancer cells.
authors.affiliation: Department of Hospital Pharmacy, Aichi Medical University School of Medicine, Nagakute-cho, Aichi-gun, Aichi 480-1195, Japan. 100019@gk.amu.aichi-med-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mori
authors.forename: Masafumi
authors.initials: M
authors.lastname: Ohnishi
authors.forename: Megumi
authors.initials: M
authors.lastname: Komiyama
authors.forename: Arisa
authors.initials: A
authors.lastname: Tsutsui
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Yabushita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Okada
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002338:Carotenoids / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology*; D019207:beta Carotene / Q000031:analogs & derivatives / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; C469955:paradicsompaprika; D019207:beta Carotene; D002338:Carotenoids