The total peroxyl radical trapping potential in serum - an assay to define the stage of atherosclerosis.
Lipid peroxides were identified among the factors that contribute to the atherosclerotic plaque formation in the arterial wall. We hypothesised that a correlation may exist between the content of antioxidant constituents in the serum and the gravity of atherosclerosis. To this purpose, we have determined the serum total peroxyl radical- trapping potential (TRAP), which is the combined capacity of all antioxidants to neutralize free radicals in serum and followed its variation in hyperlipemic animals in correlation with the stage of atherosclerosis. In addition, we compared TRAP values in the sera of coronary heart disease (CHD) patients, with or without type II diabetes mellitus. Results showed that after 18 weeks of hyperlipemic diet, the mean TRAP values measured in sera isolated from hyperlipemic hamsters exhibited an about 44% decrease, in good agreement with the increase of serum cholesterol and triglycerides. In the 3 groups of CHD patients, TRAP values decreased with about 10% in sera of stable angina patients, 20% in unstable patients, as compared with normal subjects. The lowest TRAP values were detected in the sera of patients with acute myocardial infarction. The results obtained for different experimental animals and for CHD patients sera indicate that the TRAP method, as adapted in our laboratory, is a reliable and reproducible assay, fit to be used in clinical studies as an ex vivo measurable parameter that correlates with the stage of the atherosclerosis.
Niculescu
L
L
Nicolae Simionescu Institute of Cellular Biology and Pathology, Bucharest, Romania.
Stancu
C
C
Sima
A
A
Toporan
D
D
Simionescu
M
M
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001021:Aortic Valve / Q000473:pathology; D002784:Cholesterol / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood*; D006224:Cricetinae; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D008647:Mesocricetus; D051379:Mice; D009130:Muscle, Smooth / Q000473:pathology; D010545:Peroxides / Q000097:blood*; D011817:Rabbits; D051381:Rats; D014280:Triglycerides / Q000097:blood
D000975:Antioxidants; D008054:Lipid Peroxides; D010545:Peroxides; D014280:Triglycerides; C049375:perhydroxyl radical; D002784:Cholesterol