title: Vasospasm following subarachnoid hemorrhage: evidence against functional upregulation of Rho kinase constrictor pathway.
abstract: This study tested the hypothesis that vasospasm due to subarachnoid hemorrhage involves the functional upregulation of Rho kinase. Spasm of the rabbit basilar artery was achieved using a double hemorrhage model, which we previously demonstrated was endothelin-1 dependent. In situ effects of agents were determined by direct measurement of vessel diameter following their suffusion in a cranial window. Y-27632, a Rho kinase inhibitor, relaxed the spasm. However, relaxations to Y-27632 were not significantly greater in endothelin-1 constricted spastic vessels initially relaxed with the endothelin converting enzyme inhibitor, phosphoramidon, as compared to endothelin-1 constricted control vessels. These results suggest that, at least in the rabbit double subarachnoid hemorrhage model, vasospasm does not involve the functional upregulation of Rho kinase.
authors.affiliation: Department of Pharmacology and Cell Biophyscis, University of Cincinnati College of Medicine, OH 45267-0575, USA.
authors.forename: Seonghun
authors.initials: S
authors.lastname: Yoon
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Sherman
authors.forename: Mario
authors.initials: M
authors.lastname: Zuccarello
authors.forename: Robert M
authors.initials: RM
authors.lastname: Rapoport
mesh_terms: D000577:Amides / Q000493:pharmacokinetics / Q000494:pharmacology*; D000818:Animals; D001488:Basilar Artery / Q000187:drug effects; D019332:Endothelin-1 / Q000494:pharmacology; D006020:Glycopeptides / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D009125:Muscle Relaxants, Central / Q000493:pharmacokinetics / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011725:Pyridines / Q000493:pharmacokinetics / Q000494:pharmacology*; D011817:Rabbits; D013345:Subarachnoid Hemorrhage / Q000150:complications / Q000378:metabolism; D014661:Vasoconstriction / Q000187:drug effects*; D020301:Vasospasm, Intracranial / Q000201:enzymology* / Q000209:etiology / Q000473:pathology; D054460:rho-Associated Kinases
chemical_list: D000577:Amides; D019332:Endothelin-1; D006020:Glycopeptides; D047908:Intracellular Signaling Peptides and Proteins; D009125:Muscle Relaxants, Central; D011480:Protease Inhibitors; D011725:Pyridines; C108830:Y 27632; D017346:Protein Serine-Threonine Kinases; D054460:rho-Associated Kinases; C008890:phosphoramidon