Collagen metabolism and turnover in women with stress urinary incontinence and pelvic prolapse.
The aim of this study was to investigate quantitative mRNA expression of matrix metalloproteinases MMP-1, MMP-2, MMP-9, and their inhibitors, the tissue inhibitors of metalloproteinases TIMP-1, TIMP-2 and TIMP-3, in vaginal wall tissue from women with stress urinary incontinence compared to continent controls. Vaginal wall tissues were obtained from 7 women with stress urinary incontinence/severe pelvic prolapse and 15 continent controls. RNA was then extracted and quantified. Quantitative competitive reverse transcription (QC-RT-PCR) was carried out with oligonucleotide primers to quantify MMP-1, MMP-2, MMP-9, TIMP-1, TIMP-2 and TIMP-3 mRNA expression. Stress continent women demonstrated a significant decrease in TIMP-1 and mRNA expression (P = 0.03). There was no difference in TIMP-2, TIMP-3, MMP-2 or MMP-9 mRNA expression between stress incontinent women and controls. However, MMP-1 mRNA expression was significantly increased (P = 0.05) in the incontinent group and the MMP-1/TIMP-1 ratio (P = 0.04) was consistent with increased collagen degradation in the stress incontinence. Stress incontinent women demonstrated an increase in MMP-1 mRNA expression and a decrease in the inhibitor TIMP-1 mRNA expression. Both these findings are consistent with increased collagen breakdown as a pathologic etiology of incontinence.
Chen
B H
BH
Department of Gynecology and Obstetrics, Stanford University Medical School, California 94305-5317, USA. bchen@stanford.edu
Wen
Y
Y
Li
H
H
Polan
M L
ML
D000596:Amino Acids / Q000378:metabolism; D016022:Case-Control Studies; D003094:Collagen / Q000378:metabolism*; D005260:Female; D006801:Humans; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*; D014550:Urinary Incontinence, Stress / Q000378:metabolism*; D014596:Uterine Prolapse / Q000378:metabolism*; D014621:Vagina / Q000378:metabolism
D000596:Amino Acids; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; C015484:pyridinoline; D003094:Collagen; D020782:Matrix Metalloproteinases