Prevalence of virulence genes in Escherichia coli strains isolated from Romanian adult urinary tract infection cases.
A total of 78 E. coli strains isolated from adults with different types of urinary tract infections were screened by polymerase chain reaction for prevalence of genetic regions coding for virulence factors. The targeted genetic determinants were those coding for type 1 fimbriae (fimH), pili associated with pyelonephritis (pap), S and F1C fimbriae (sfa and foc), afimbrial adhesins (afa), hemolysin (hly), cytotoxic necrotizing factor (cnf), aerobactin (aer). Among the studied strains, the prevalence of genes coding for fimbrial adhesive systems was 86%, 36%, and 23% for fimH, pap, and sfa/foc,respectively. The operons coding for Afa afimbrial adhesins were identified in 14% of strains. The hly and cnf genes coding for toxins were amplified in 23% and 13% of strains, respectively. A prevalence of 54% was found for the aer gene. The various combinations of detected genes were designated as virulence patterns. The strains isolated from the hospitalized patients displayed a greater number of virulence genes and a diversity of gene associations compared to the strains isolated from the ambulatory subjects. A rapid assessment of the bacterial pathogenicity characteristics may contribute to a better medical approach of the patients with urinary tract infections.
Usein
C R
CR
Molecular Epidemiology Laboratory, Cantacuzino Institute, Bucharest, Romania. molepi@cantacuzino.ro
Damian
M
M
Tatu-Chitoiu
D
D
Capusa
C
C
Fagaras
R
R
Tudorache
D
D
Nica
M
M
Le Bouguénec
C
C
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012383:Romania; D014552:Urinary Tract Infections / Q000382:microbiology*; D014774:Virulence / Q000235:genetics*
D017931:DNA Primers; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins