title: Effect of temperature, storage time, and sample type on sorbitol dehydrogenase activity in llama serum and plasma.
abstract: Serum and heparinized plasma samples were collected from 11 adult, clinically healthy llamas. Aliquots were assayed for sorbitol dehydrogenase (SDH) activity after storage at room temperature (20 degrees C), 4 degrees C, or -20 degrees C for defined time intervals up to 1 week postcollection. Sorbitol dehydrogenase activity in all samples was within reference intervals for our laboratory. No difference was found between serum and plasma SDH activity when measured immediately (within 1 hour) after collection. Sorbitol dehydrogenase activity decreased to 79% of initial activity by 24 hours in serum stored at room temperature; plasma had 94% of initial SDH activity under the same conditions. Sorbitol dehydrogenase activity was stable in both plasma and serum stored for up to 1 week at 4 degrees C or -20 degrees C. With the exception of serum stored at 20 degrees C for > 8 hours, in vitro stability of llama SDH was adequate or its use in diagnostic testing.
authors.affiliation: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA. susan.tornquist@orst.edu
authors.forename: Susan J.
authors.initials: SJ
authors.lastname: Tornquist
authors.forename: Linda
authors.initials: L
authors.lastname: Dodson
authors.forename: Dirk V.
authors.initials: DV
authors.lastname: Lanning