Optimization of a rapid and sensitive identification system for Salmonella enteritidis by capillary electrophoresis with laser-induced fluorescence.
A system coupling capillary electrophoresis (CE) with a laser-induced fluorescence (LIF) system for the identification of Salmonella enteritidis was developed. Addition of an appropriate amount of sodium alginate and NaCl to the running buffer made it possible to obtain a reproducible sharp peak. Two fluorescent staining methods using a cell-permeable nucleic acid stain and a salmonellae-specific polyclonal antibody were adapted to the system. The CE-LIF successfully detected as few as three cells per injection from a pure culture of S. enteritidis. The CE-LIF system can be conveniently used for rapid and highly sensitive identification of S. enteritidis.
Shintani
Tomoyoshi
T
Industrial Research Center of Ehime Prefecture, 487-2 Kumekubota, Matsuyama, Ehime 791-1101, Japan. shintani@iri.pref.ehime.jp
Yamada
Kazutaka
K
Torimura
Masaki
M
D000464:Alginates / Q000378:metabolism; D000907:Antibodies, Bacterial / Q000032:analysis; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007834:Lasers; D015203:Reproducibility of Results; D012477:Salmonella enteritidis / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors
D000464:Alginates; D000907:Antibodies, Bacterial; D002021:Buffers; D006603:Hexuronic Acids; D020723:Glucuronic Acid