Comparison of techniques permitting to detect apoptosis in situ.
Several morphological and biochemical techniques are in use for identification of apoptotic and necrotic cells in a studied cell population. It is essential to define not only the type of cell death but also to identify the apoptotic process itself, which represents a multistage, active process, requiring activation of a molecular event cascade. In the present study, we have examined and discussed effectiveness of the selected techniques detecting apoptosis in lymphocytes exposed to incubation at an elevated temperature. The appraisal involved detection of caspase-3 active form, detection of Bcl-2, TUNEL reaction, the comet assay and electrophoresis of DNA.
Drag-Zalesińska
M
M
Department of Histology and Embryology, University Medical School, Wrocław, Poland.
Wysocka
T
T
Dumańska
M
M
Jagoda
E
E
Zabel
M
M
D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3; D020169:Caspases / Q000032:analysis / Q000378:metabolism; D020552:Comet Assay; D003584:Cytological Techniques; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013696:Temperature
D019253:Proto-Oncogene Proteins c-bcl-2; D004247:DNA; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases