Human leucocyte antigen-Cw-specific cytotoxic T lymphocytes generated from naive cord blood used for cord blood stem cell transplantation.
Human leucocyte antigen (HLA)-Cw-reactive cytotoxic T lymphocytes (CTL) were generated from cord blood (CB) lymphocytes of two cases used for cord blood stem cell transplantation (CBSCT). In both cases, the CTL were cytotoxic against the patient's leukaemic cells, as well as the patient's Epstein-Barr virus (EBV)-lymphoblastoid cell line (EBV-LCL) and phytohaemagglutinin blasts, and the cytotoxicity was blocked by anti-HLA-class I monoclonal antibodies. In the first case, the CTL recognized Cw 3 (Cw 9 and Cw 10)-positive EBV-LCL, while in the second case, the CTL recognized Cw1 and/or Cw7. These cases suggest that CB T cells may be competent enough for generating CTL to induce a graft-versus-leukaemia effect and/or graft-versus-host disease in patients with CBSCT and that the mismatching of Cw antigens between patient and CB may be related to the outcome of CBSCT.
Yazaki
Makoto
M
Department of Paediatrics, Nagoya City University Medical School, 1-Kawasumi, Mizuho-cho, Nagoya 467-8601, Japan. yazaki@med.nagoya-cu.ac.jp
Takahashi
Toshitada
T
Mizutani
Keigo
K
Ito
Yasuhiko
Y
Wakiguchi
Hiroshi
H
Inoue
Masami
M
Kawa
Keisei
K
Kato
Koji
K
Kato
Toro
T
Saito
Hidehiko
H
Togari
Hajime
H
D000208:Acute Disease; D002469:Cell Separation; D002675:Child, Preschool; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005312:Fetal Blood / Q000276:immunology*; D006087:Graft vs Host Reaction / Q000276:immunology; D020544:Graft vs Leukemia Effect / Q000276:immunology; D015236:HLA-C Antigens / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D004854:Herpesvirus 4, Human; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology / Q000821:virology; D007951:Leukemia, Myeloid / Q000276:immunology / Q000601:surgery; D008297:Male; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000601:surgery; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015236:HLA-C Antigens; C038653:HLA-C*03 antigen; C040178:HLA-C*70 antigen; C047431:HLA-Cw1 antigen