Structure of the O-polysaccharide of Pseudomonas syringae pv. delphinii NCPPB 1879(T) having side chains of 3-acetamido-3,6-dideoxy-D-galactose residues.
The O-polysaccharide (OPS) was obtained from the lipopolysaccharide of Pseudomonas syringae pv. delphinii NCPPB 1879(T) and studied by sugar and methylation analyses, Smith degradation, and (1)H- and (13)C-NMR spectroscopy. The OPS was found to contain residues of L-rhamnose (L-Rha) and 3-acetamido-3,6-dideoxy-D-galactose (D-Fuc3NAc), and the following structure of the major (n = 2) and minor (n = 3) heptasaccharide repeating units of the OPS was established: [carbohydrate structure: see text]. The OPS is distinguished by the presence of oligosaccharide side chains consisting of three D-Fuc3NAc residues that are connected to each other by the (alpha 1-->2)-linkage. The OPS is characterized by a structural heterogeneity due to a different position of substitution of one of the four L-rhamnose residues in the main chain of the repeating unit as well as to the presence of oligosaccharide units with an incomplete side chain.
Zdorovenko
E L
EL
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia.
Zatonsky
G V
GV
Kocharova
N A
NA
Shashkov
A S
AS
Knirel
Y A
YA
Ovod
V V
VV
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005690:Galactose / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification*; D011549:Pseudomonas / Q000737:chemistry*
D008070:Lipopolysaccharides; D019081:O Antigens; D005690:Galactose