In silico analyses of mouse inner-ear transcripts.
The development and function of the inner ear is complex requiring the correct and coordinated expression of many genes. The recent progress in the analyses of the human and other genomes has provided tools for identification of genes involved in hearing. As more and more nucleotide sequence information accumulates, experimental methods of molecular biology are rapidly being supplemented, and partially supplanted, by computational methods. In this study we present comprehensive in silico analyses of a cDNA library representing almost 1600 transcripts isolated from mouse inner ear. By mining the public databases we were able to rapidly and efficiently identify numerous transcripts likely to have a specific role in the auditory or vestibular function of the inner ear. Analyses revealed about 600 known genes and almost 100 inner-ear specific transcripts. Almost 50 of these are candidate genes for hearing impairment based on their chromosomal localization and inner-ear expression pattern. We describe a powerful approach to identify novel genes associated with hearing and vestibular function, further increasing our understanding of the molecular biology of the inner ear.
Klockars
Tuomas
T
Murdoch Childrens Research Institute, Department of Paediatrics, University of Melbourne, The Royal Children's Hospital, Parkville, Victoria, Australia. klockarst@murdoch.rch.unimelb.edu.au
Perheentupa
Teemu
T
Dahl
Hans-Henrik M
HH
D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D007758:Ear, Inner / Q000502:physiology*; D015723:Gene Library; D016678:Genome; D006309:Hearing / Q000235:genetics*; D051379:Mice; D009928:Organ Specificity; D011506:Proteins / Q000235:genetics; D014158:Transcription, Genetic
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D011506:Proteins