[Production and analytical properties of antibodies with high specificity to zearalenone].
The time course of production, specificity, and analytical potential of anti-zearalenone polyclonal rabbit antibody synthesized by formaldehyde condensation and conjugated to bovine serum albumin were investigated. The relative cross-reactivities with natural analogues were: zearalenone, 100%; alpha-zearalenol, 0.15%; and beta-zearalenol, < 0.02%. With synthetic analogues: zearalanone, 31.7% and alpha-zearalanol, 0.12%. An enzyme immunoassay for zearalenone with a sensitivity of 0.1 ng/ml was developed on the basis of these antibodies and solid-phase conjugates homologous to the immunogen in the method of synthesis.
Burkin
A A
AA
All-Russia Research Institute of Veterinary Sanitation, Hygiene, and Ecology, Russian Academy of Agricultural Sciences, Moscow, 123022 Russia.
Kononenko
G P
GP
Soboleva
N A
NA
D000276:Adjuvants, Immunologic; D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis / Q000276:immunology*; D003429:Cross Reactions; D005557:Formaldehyde / Q000276:immunology; D007124:Immunoenzyme Techniques; D008297:Male; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D015025:Zearalenone / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000908:Antibodies, Fungal; D005557:Formaldehyde; D012710:Serum Albumin, Bovine; D015025:Zearalenone
Poluchenie i analiticheskie svoĭstva antitel s vysokoĭ spetsifichnost'iu k zearalenonu.