Lactobacillus durianis sp. nov., isolated from an acid-fermented condiment (tempoyak) in Malaysia.
Lactic acid bacteria (LAB) are the predominant micro-organisms in tempoyak, a Malaysian acid-fermented condiment. In a study on the diversity of LAB in this product, three isolates could not be identified using SDS-PAGE of whole-cell proteins or API 50 CH. The taxonomic position of the three isolates was clarified in the present study. 16S rDNA sequencing classified a representative strain in the genus Lactobacillus, clearly separated from all known species, and most closely related to the Lactobacillus reuteri phylogenetic group. DNA-DNA hybridization experiments and an extensive phenotypic description confirm that the strains represent a single and separate novel species among the obligately heterofermentative lactobacilli. The three isolates are distinguished at the intra-species level by plasmid profiling, pulsed-field gel electrophoresis of macro-restriction fragments and biochemical features. The name Lactobacillus durianis sp. nov. is proposed for the novel taxon and the type strain is LMG 19193T (= CCUG 45405T).
Leisner
J J
JJ
Vancanneyt
M
M
Lefebvre
K
K
Vandemeulebroecke
K
K
Hoste
B
B
Vilalta
N Euras
NE
Rusul
G
G
Swings
J
J
D004275:DNA, Ribosomal / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005285:Fermentation; D005638:Fruit / Q000382:microbiology*; D019344:Lactic Acid / Q000378:metabolism*; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D008296:Malaysia; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid