title: Effects of in utero retinoic acid exposure on mouse pelage hair follicle development.
abstract: We investigated in vivo the histological and immunohistochemical responses of mouse hair pelage follicle morphogenesis to prenatal exposure to a potentially nonteratogenic dose of all-trans-retinoic acid (RA), as a basis studying the preventive effect of RA on adult mouse skin carcinogenesis. In pregnant mice, a single oral dose of RA at 30 mg kg-1 body weight given on day 11.5 of gestation caused no RA-induced changes in the morphology or temporal expression patterns of keratins during pelage hair follicle morphogenesis. The only differential effect of RA was a statistically significant increase in the number of BrdU-positive nuclei in hair bulbs from RA exposed fetuses compared with nonexposed mice. The absence of adverse RA effects suggests that this experimental design may represent a valuable protocol for use in studies on the in vivo effects of this retinoid on different skin diseases.
authors.affiliation: Histology and Pathological Anatomy Section, Department of Animal Pathology, Animal Medicine, Faculty of Veterinary Science, University of León, Spain.
authors.forename: Rosa A
authors.initials: RA
authors.lastname: García-Fernández
authors.forename: Claudia
authors.initials: C
authors.lastname: Pérez-Martínez
authors.forename: Alfredo
authors.initials: A
authors.lastname: Escudero-Diez
authors.forename: Maria J
authors.initials: MJ
authors.lastname: García-Iglesias
mesh_terms: D000818:Animals; D005260:Female; D018859:Hair Follicle / Q000187:drug effects* / Q000196:embryology*; D007150:Immunohistochemistry / Q000662:veterinary; D007641:Keratolytic Agents / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D009024:Morphogenesis; D011247:Pregnancy; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D007641:Keratolytic Agents; D014212:Tretinoin