Novel cDNAs encoding salivary proteins from the malaria vector Anopheles gambiae.
Several genes encoding salivary components of the mosquito Anopheles gambiae were identified using a selective trapping approach. Among these, five corresponded to genes expressed specifically in female glands and their role may possibly be linked to blood-feeding. Our collection included a fourth member of the D7 protein family and two polypeptides that showed weak similarity to anti-coagulants from distantly related species. Moreover, we identified two additional members of a novel group of proteins that we named glandins. The isolation of tissue-specific genes represents a first step toward a deeper molecular analysis of mosquito salivary secretions.
Lanfrancotti
Alessandra
A
Department of Public Health, Parasitology Unit, University La Sapienza, Rome, Italy.
Lombardo
Fabrizio
F
Santolamazza
Federica
F
Veneri
Massimiliano
M
Castrignanò
Tiziana
T
Coluzzi
Mario
M
Arcà
Bruno
B
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics*; D007303:Insect Vectors / Q000235:genetics*; D008288:Malaria; D008297:Male; D008969:Molecular Sequence Data; D012469:Salivary Glands / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000096:biosynthesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D019476:Insect Proteins; D012471:Salivary Proteins and Peptides