Targeting peptides for microglia identified via phage display.
Screening with a 7-mer phage display peptide library, a panel of cell-targeting peptides for the murine microglial cell line, EOC 20, was recognized. A number of similar, but not identical, sets of sequences representing more than 75% of all the cell line-binding clones were identified. Comparative analysis indicated that motif S/(T) F T/(X) Y W is present in the vast majority of the binding sequences. The selectivity and specificity of the dominant peptide sequence identified for microglia was confirmed using both phage displaying the peptide and the synthetic peptide alone.
Samoylova
Tatiana I
TI
Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA. samoiti@vetmed.auburn.edu
Ahmed
Bushra Y
BY
Vodyanoy
Vitaly
V
Morrison
Nancy E
NE
Samoylov
Alexandre M
AM
Globa
Ludmila P
LP
Baker
Henry J
HJ
Cox
Nancy R
NR
D000818:Animals; D015374:Biosensing Techniques; D002460:Cell Line; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000378:metabolism*; D010446:Peptide Fragments / Q000235:genetics* / Q000378:metabolism*; D019151:Peptide Library; D011485:Protein Binding / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity
D010446:Peptide Fragments; D019151:Peptide Library