title: Isolation and identification of antioxidants from Sophora japonica.
abstract: A new flavonol triglycoside, kaempferol 3-O-alpha-L-rhamnopyranosyl-(1 --> 6)-beta-D-glucopyranosyl-(1 --> 2)-beta-D-glucopyranoside (1), as well as two known kaempferol 3-O-[alpha-L-rhamnopyranosyl-(1 --> 6)]-[beta-D-glucopyranosyl-(1 --> 2)]-beta-D-glucopyranoside (2) and kaempferol 3-O-beta-D-glucopyranosyl-(1 --> 2)-beta-D-glucopyranoside-7-O-alpha-L-rhamnopyranoside (3), were isolated from the n-BuOH extract of the pericarps of Sophora japonica by bioassay-guided fractionation. The structure of compound 1 was established by UV, IR, MS, and one- and two-dimensional NMR spectroscopy, including DEPT, NOESY, DQF-COSY, TOCSY, HMQC, and HMBC experiments. Compounds 1-3 showed antioxidative activity in DPPH and cytochrome-c assay using HL-60 cell system.
authors.affiliation: Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing. yptang@pub.sioc.ac.cn
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Tang
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Hu
authors.forename: Feng-Chang
authors.initials: FC
authors.lastname: Lou
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001681:Biological Assay; D003574:Cytochrome c Group / Q000187:drug effects; D013058:Mass Spectrometry; D010946:Plants, Medicinal / Q000737:chemistry*; D029909:Sophora / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D000975:Antioxidants; D003574:Cytochrome c Group