Identification of human L-fucose kinase amino acid sequence.
Fucose is a major component of complex carbohydrates. L-Fucose kinase (fucokinase) takes part in the salvage pathway for reutilization of fucose from the degradation of oligosaccharides. The amino acid sequence of human fucokinase was derived from a cDNA encoding a protein of hitherto unidentified function. Human fucokinase polypeptide chain consists of 990 amino acids with a predicted molecular mass of 107 kDa. The C-terminal part of its amino acid sequence showed sequence motifs typical for sugar kinases. Fucokinase full-length protein and a deletion mutant lacking the first 363 amino acids of the N-terminus were expressed in Escherichia coli BL21 cells. Both proteins displayed fucokinase activity. These results reveal that the discovered cDNA encodes the fucokinase protein and they confirm that a functional kinase domain is located in the C-terminal part of the enzyme.
Hinderlich
Stephan
S
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, 14195 Berlin-Dahlem, Germany. hinderli@zedat.fu-berlin.de
Berger
Markus
M
Blume
Astrid
A
Chen
Hao
H
Ghaderi
Darius
D
Bauer
Christian
C
D000255:Adenosine Triphosphate / Q000737:chemistry; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry* / Q000235:genetics; D020539:Sequence Analysis, Protein
D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C020153:fucokinase