[Newly isolated mink parvovirus strain].
A new mink enteritis virus (MEV) strain, called Cherepanovo, was isolated in the Novosibirsk region (West Siberia). The level of this strain's accumulation in cell culture is lower than that of commercial MEV strains Rodniki or Beregovoi. Identification of Cherepanovo strain by hemagglutination inhibition test, protein electrophoresis in denaturing polyacrylamide gel, and PCR showed its similarity to the previously described MEV strains. Comparative analysis of this strain's 5' and 3' nucleotide sequences of VP2 gene showed its more than 98% homology with other feline parvoviruses. Nucleotide residues 2920, 2971, and 4245 in the Cherepanovo strain VP2 gene differed from those of all previously described MEV strains. It is noteworthy that transition at the position 4245 led to Phe-->Cys replacement in amino acid residue.
Tkachev
S E
SE
Sigitova
E V
EV
Mitrofanova
E E
EE
Durymanov
A G
AG
Shestopalov
A M
AM
D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D006385:Hemagglutination Inhibition Tests; D008969:Molecular Sequence Data; D017992:Parvovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012800:Siberia
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D036022:Capsid Proteins
Novyĭ izoliat parvovirusa norok.