Suppression of IgE antibody response in mice by a polysaccharide, AZ9, produced by Klebsiella oxytoca strain TNM3.
A soil bacterium, Klebsiella oxytoca TNM3 was found to produce a polysaccharide named AZ9 that shows suppressive effects on IgE antibody response in mice. When mice were administered with 50 to approximately 100 mg/kg AZ9 subcutaneousely for 4 consecutive days after immunization with trinitrophenyl (TNP)-keyhole limpet hemocyanin, anti-TNP IgE production was significantly suppressed, while the level of anti-TNP IgM was affected marginally. In AZ9-administered mice, IL-4 secretion from splenic cells was reduced to approximately 30% of the untreated control. Thus, AZ9 suppression of IgE production may be due to attenuating effects on the Th2-type response. Although oral administration of AZ9 alone had no effects on IgE production, ovalbumin (OVA)-induced oral tolerance of anti-TNP IgE response to TNP-OVA was markedly augmented when a suboptimal dose of OVA was administered orally in combination with AZ9. Collectively, our data suggest that AZ9 has beneficial suppressive effects on IgE-dependent allergic responses.
Sugihara
Ryosuke
R
Tayca Corporation, Osaka, Japan.
Matsumoto
Yuki
Y
Ohmori
Hitoshi
H
D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D006241:Haptens; D006433:Hemocyanins / Q000008:administration & dosage / Q000276:immunology; D007108:Immune Tolerance / Q000187:drug effects; D007114:Immunization; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D041121:Klebsiella oxytoca / Q000737:chemistry* / Q000276:immunology*; D008297:Male; D051379:Mice; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology*
D000941:Antigens; D006241:Haptens; D007075:Immunoglobulin M; D007166:Immunosuppressive Agents; D011135:Polysaccharides, Bacterial; C054401:trinitrophenyl keyhole limpet hemocyanin; C038600:trinitrophenyl-ovalbumin; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin; D006433:Hemocyanins