Furocoumarins from Angelica dahurica with hepatoprotective activity on tacrine-induced cytotoxicity in Hep G2 cells.
Fractionation of the MeOH extract of Angelica dahurica Benth et Hook resulted in the isolation of six furocoumarins, imperatorin (1), isoimperatorin (2), (+/-)-byakangelicol (3), (+)-oxypeucedanin (4), (+)-byakangelicin (5), and (+)-aviprin (6). Among these, compounds 1 and 5 exhibited strong hepatoprotective activities, displaying EC(50) values of 36.6 +/- 0.98 and 47.9 +/- 4.6 microM, respectively. Compounds 3 and 4 showed moderate activities with EC(50) values of 112.7 +/- 5.35 and 286.7 +/- 6.36 microM, respectively. Silybin as a positive control showed the EC(50) value with 69.0 +/- 3.4 microM. Comparison of hepatoprotective activities for six furocoumarins 1 - 6 suggested that oxy-substitution at the C-9 position increased the hepatoprotective activity.
Oh
Hyuncheol
H
Lee
Ho-Sub
HS
Kim
Taewan
T
Chai
Kyu-Yun
KY
Chung
Hun-Taeg
HT
Kwon
Tae-Oh
TO
Jun
Jung-Yang
JY
Jeong
Ok-Sam
OS
Kim
Youn-Chul
YC
Yun
Young-Gab
YG
D029969:Angelica; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011564:Furocoumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008518:Medicine, East Asian Traditional; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D013619:Tacrine / Q000633:toxicity; D014407:Tumor Cells, Cultured / Q000187:drug effects
D003374:Coumarins; D005663:Furans; D011564:Furocoumarins; D010936:Plant Extracts; C462035:aviprin; C055542:isoimperatorin; C462034:byakangelicol; C434685:9-(2,3-dihydroxy-3-methylbutoxy)-4-methoxy-7H-furo(3,2-g)(1)benzopyran-7-one; D013619:Tacrine; C031534:imperatorin; C031535:oxypeucadanin