title: Structures of two major histocompatibility complex class I genes of the rainbow trout (Oncorhynchus mykiss).
abstract: Here we describe two rainbow trout major histocompatibility complex (MHC) class I genes characterized from lambda phage genomic clones prepared from a single fish. Clone GC71 contains all exons except a leader peptide-encoding exon. An open reading frame is maintained, and thus the gene MhcOnmy-U71 could be expressed in this individual. The class I gene found on clone GC41 lacks exons encoding the leader peptide and cytoplasmic domain. This gene, MhcOnmy-U41p, is a pseudogene due to a deletion in the alpha(2) domain-encoding exon causing premature termination. Both the Onmy-U71 and Onmy-U41p genes are distinguished by long introns between the exons encoding the alpha(1) and alpha(2) domains. Clone GC41 also contains the 3' exons of the LMP7/ PSMB8 gene encoding the gamma-interferon-induced proteosome subunit of rainbow trout.
authors.affiliation: Department of Structural Biology, Sherman Fairchild Building, Stanford University School of Medicine, Stanford, CA 94305-5126, USA.
authors.forename: Benny P
authors.initials: BP
authors.lastname: Shum
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Mason
authors.forename: Katharine E
authors.initials: KE
authors.lastname: Magor
authors.forename: Laura R
authors.initials: LR
authors.lastname: Flodin
authors.forename: René J M
authors.initials: RJ
authors.lastname: Stet
authors.forename: Peter
authors.initials: P
authors.lastname: Parham
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D029941:Fish Proteins / Q000235:genetics*; D005805:Genes, MHC Class I; D008040:Genetic Linkage; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D010802:Phylogeny; D011544:Pseudogenes; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D029941:Fish Proteins; D015395:Histocompatibility Antigens Class I