Genes coding structural proteins in the Leishmania braziliensis complex.
Acidic ribosomal P1 and P2b proteins, referred to as P proteins, and histone H3 are reported for first time in the Leishmania braziliensis complex. Deoxyribonucleic acid analysis and multiple sequence alignment suggest that both P proteins may maintain their structural function in the ribosomal stalk, in spite of the high rate of mutations detected. The deduced amino acid sequence of protein P1 showed 51% identity with Trypanosoma cruzi protein P1 and protein P2b showed 61% identity with T. cruzi protein P2b. Another conserved protein, L. (Viannia) braziliensis histone H3, showed 82% and 70% identity with histone H3 of L. (Leishmania) infantum and T. cruzi, respectively. The N-terminal end of this histone is divergent in comparison with the consensus eukaryotic sequence. Their predicted tridimensional structure was designed.
Padilla
C
C
Instituto Nacional de Salud, Centro Nacional de Laboratorios de Salud Pública, Jr Capac Yupanqui 1400, Jesus Maria, Lima, Perú.
Barreto
T
T
De Los Santos
M
M
Barker
D C
DC
Carrillo
C
C
Montoya
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D016054:DNA, Protozoan / Q000235:genetics; D017125:Genes, Protozoan; D006657:Histones / Q000235:genetics; D007892:Leishmania braziliensis / Q000235:genetics*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics
D016054:DNA, Protozoan; D006657:Histones; D010750:Phosphoproteins; D015800:Protozoan Proteins; D012269:Ribosomal Proteins; C031937:phosphoprotein P2, ribosomal; C035690:ribosomal phosphoprotein P1