Inhibition of Scavenger Receptor A Expression Treated with PMA by the Inhibitor of Tyrosine Protein Kinase Genistein.
In order to investigate the relationship between scavenger receptor type A and cell signal transduction, human U937 macrophages were treated with tyrosine protein kinase inhibitor genistein, then the cells were incubated with [(125)I]ox-LDL or ox-LDL, and the cellular degradation of [(125)I]ox-LDL or binding were measured separately. Then the effect of the drug on cell surface expression of SR-A were measured by means of autoradiography, it was found that genistein could reduce cellular SR-A mRNA transcription by RT-PCR. The results indicated that genistein could reduce U937 macrophages to bind lipids and reduce SR-A expression by suppression of transcription, and could reduce degradation of lipids by U937 macrophage and accumulation of cholesterol within the cells. It suggests that the function of scavenger receptors may be correlated with cell tyrosine protein kinase, the mechanism is transcriptional and it also suggests that SR-A may participate in the signal transduction directly.
Jin
Yan
Y
Atherosclerosis Research Center, Nanjing Medical University, Nanjing 210029, China. q.Chen@public.ptt.js.cn
Chen
Qi
Q
Wei
En-Hui
EH
Jiang
Li
L
Fan
Le-Ming
LM
Wang
Nan
N
Chen
Xiu-Ying
XY