The content and chain length of polyphosphates from vacuoles of Saccharomyces cerevisiae VKM Y-1173.
The content of inorganic polyphosphates (polyP) in vacuoles of the yeast Saccharomyces cerevisiae is approximately 15% of the total cellular polyP. Over 80% of the vacuole polyP are in an acid-soluble fraction. It was first established by (31)P-NMR spectroscopy that a polymeric degree (n) of two subfractions obtained by precipitation with Ba(2+) in succession at pH 4.5 and 8.2 was approximately 20 +/- 5 and 5 +/- 2 residues of ortho-phosphoric acid, respectively. Under a deficit of phosphate (P(i)) in the cultivation medium, the polyP content in vacuoles decreased approximately 7-fold with the same drastic reduction of their content in the cell. Unlike intact yeast cells, where polyP overcompensation is observed after their transfer from phosphate-free to phosphate-containing medium, the vacuoles do not show this effect. The data indicate the occurrence of special regulatory mechanisms of polyP synthesis in vacuoles differing from those in the whole cell.
Trilisenko
L V
LV
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. LVAT@ibpm.serpukhov.su
Vagabov
V M
VM
Kulaev
I S
IS
D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D010316:Particle Size; D011122:Polyphosphates / Q000032:analysis / Q000737:chemistry*; D011523:Protoplasts / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000166:cytology* / Q000201:enzymology; D017421:Sequence Analysis; D025262:Vacuolar Proton-Translocating ATPases / Q000302:isolation & purification; D014617:Vacuoles / Q000737:chemistry* / Q000201:enzymology
D011122:Polyphosphates; D025262:Vacuolar Proton-Translocating ATPases