Yop fusions to tightly folded protein domains and their effects on Yersinia enterocolitica type III secretion.
Yersinia enterocolitica organisms secrete Yop proteins via the type III pathway. Translational fusion of yop genes to ubiquitin or dihydrofolate reductase results in hybrid proteins that cannot be secreted. The folding of hybrids prevents their own transport, but it does not hinder the type III secretion of other Yops.
Lee
Vincent T
VT
Department of Microbiology & Immunology, University of California Los Angeles School of Medicine, Los Angeles, California 90095, USA.
Schneewind
Olaf
O
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D003062:Codon; D017510:Protein Folding; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism; D015008:Yersinia enterocolitica / Q000235:genetics / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D003062:Codon; D011993:Recombinant Fusion Proteins; D025801:Ubiquitin; C063239:yopE protein, Yersinia; C067570:YopQ protein, Yersinia enterocolitica; D013762:Tetrahydrofolate Dehydrogenase