Physical association of beta 2 integrin with GPI-80, a novel glycosylphosphatidylinositol-anchored protein with potential for regulating adhesion and migration.
We recently found a novel GPI-anchored protein, GPI-80, on human phagocytes that may regulate leukocyte adherence, locomotion, and extravasation. To clarify the mechanisms by which GPI-80 functions on leukocytes, we explored the possibility of its physical association with beta 2 integrin which is important for leukocyte adherence, locomotion, and extravasation. beta 2 integrin, detected by anti-CD18 mAb, was coprecipitated with GPI-80 from human neutrophil lysates by a mAb to GPI-80. In addition, GPI-80 was immunoprecipitated from human neutrophil lysates by anti-human CD18 mAb. These results clearly show that GPI-80 is physically associated with beta 2 integrin in human neutrophils.
Watanabe
Tadashi
T
Department of Immunology and Parasitology, Yamagata University School of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan.
Sendo
Fujiro
F
D000581:Amidohydrolases; D018821:CD18 Antigens / Q000737:chemistry / Q000502:physiology*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000502:physiology*; D002465:Cell Movement; D058851:GPI-Linked Proteins; D006801:Humans; D006867:Hydrolases; D009504:Neutrophils / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D011233:Precipitin Tests; D011485:Protein Binding
D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D006867:Hydrolases; D000581:Amidohydrolases; C121771:VNN2 protein, human