title: Penicillin G acylase-fatty lipid biocomposite films show excellent catalytic activity and long term stability/reusability.
abstract: The formation of biocomposite films of the pharmaceutically important enzyme penicillin G acylase (PGA) and fatty lipids under enzyme-friendly conditions is described. The approach involves a simple beaker-based diffusion protocol wherein the enzyme diffuses into the lipid film during immersion in the enzyme solution, thereby leading to the formation of a biocomposite film. The incorporation of the enzyme in both cationic as well as anionic lipids suggests the important role of secondary interactions such as hydrophobic and hydrogen bonding in the enzyme immobilization process. The kinetics of formation of the enzyme-lipid biocomposites has been studied by quartz crystal microgravimentry (QCM) measurements. The stability of the enzyme in the lipid matrix was confirmed by Fourier transform infrared spectroscopy (FTIR) and biocatalytic activity measurements. Whereas the biological activity of the lipid-immobilized enzyme was marginally higher than that of the free enzyme, the biocomposite film exhibited increased thermal/temporal stability. Particularly exciting was the observation that the biocomposite films could be reused in biocatalysis reactions without significant loss in activity, which indicates potentially exciting biomedical/industrial application of these films.
authors.affiliation: Materials Chemistry and Biochemical Sciences Divisions, National Chemical Laboratory, Pune 411 008, India.
authors.forename: Sumant
authors.initials: S
authors.lastname: Phadtare
authors.forename: Parag
authors.initials: P
authors.lastname: Parekh
authors.forename: Anand
authors.initials: A
authors.lastname: Gole
authors.forename: Mrunal
authors.initials: M
authors.lastname: Patil
authors.forename: Archana
authors.initials: A
authors.lastname: Pundle
authors.forename: Asmita
authors.initials: A
authors.lastname: Prabhune
authors.forename: Murali
authors.initials: M
authors.lastname: Sastry
mesh_terms: D002384:Catalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D050356:Lipid Metabolism; D008567:Membranes, Artificial; D010399:Penicillin Amidase / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D010399:Penicillin Amidase