Extraction and analysis of microcystins RR and LR in cyanobacteria using a cyano cartridge.
A new method for the extraction of microcystins RR and LR in cyanobacteria was developed using a cyano cartridge. Lyophilized cells (100 mg) were extracted with 5% (v/v) acetic acid. The extract was centrifuged and then the supernatant was applied to a CN cartridge. The cartridge that contained microcystins was rinsed with 5 ml of water and 5 ml of 0.5 M acetic acid, followed by 5 ml of 5% acetonitrile in water. Microcystins were finally eluted from the CN cartridge with 70% acetonitrile in water and were determined by HPLC. Better recoveries and chromatograms were observed than with ODS cartridges.
Pyo
Dongjin
D
Department of Chemistry, Kangwon National University, Chuncheon 200-701, South Korea.
Shin
Hyundu
H
D019342:Acetic Acid / Q000737:chemistry*; D000097:Acetonitriles / Q000737:chemistry; D001671:Biochemistry / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000378:metabolism*; D005612:Freeze Drying; D052998:Microcystins; D008956:Models, Chemical; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification*
D000097:Acetonitriles; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin; D019342:Acetic Acid; C032159:acetonitrile