Urinary cytology associated with human polyomavirus and indinavir therapy in HIV-infected patients.
We retrospectively analyzed 155 urine cytology samples (78 from patients treated with indinavir; 77, no indinavir) from 90 HIV+ patients to evaluate possible association between human polyomavirus and hematuria and to describe indinavir-associated urinary cytologic findings. The CD4 count also was recorded. Variables studied included the presence of cellular viral changes consistent with polyomavirus infection (PVCs), microscopic hematuria, multinucleated cells, indinavir crystals, neutrophils, and eosinophils. Twenty-two samples (15.8%) from patients with CD4 counts of more than 200/microL (>200 x 10(6)/L) showed PVCs. Multinucleated cells, of presumed histiocytic origin based on morphologic features and selective immunocytochemical findings, were present in a higher percentage of samples from indinavir-treated patients. Neutrophils were present in a higher percentage of indinavir-treated patients. Indinavir crystals were identified in 9 samples (12%) from patients receiving indinavir The lower percentage of PVCs in HIV+ patients with high CD4 counts likely represents an indirect antipolyomavirus indinavir effect by boosting immunity. Multinucleated cells (presumably histiocytic) and acute inflammation are associated with indinavir therapy. Indinavir crystals have a characteristic fan or circular lamellate appearance. Because indinavir crystals may be associated with genitourinary disease, recognizing and reporting them is clinically relevant in HIV+ patients.
Filie
Armando C
AC
Cytopathology Section, Laboratory of Pathology, National Cancer Institute, Bethesda, MD 20892, USA.
Wilder
Anna Maria
AM
Brosky
Keith
K
Kopp
Jeffrey B
JB
Miller
Kirk D
KD
Abati
Andrea
A
D001739:BK Virus / Q000302:isolation & purification*; D018791:CD4 Lymphocyte Count; D003460:Crystallization; D005260:Female; D015726:Giant Cells / Q000473:pathology; D015658:HIV Infections / Q000150:complications / Q000188:drug therapy* / Q000473:pathology / Q000652:urine*; D017320:HIV Protease Inhibitors / Q000627:therapeutic use*; D006417:Hematuria / Q000209:etiology / Q000473:pathology / Q000652:urine; D006801:Humans; D019469:Indinavir / Q000627:therapeutic use*; D008297:Male; D009504:Neutrophils / Q000473:pathology; D027601:Polyomavirus Infections / Q000150:complications / Q000473:pathology / Q000652:urine*; D012189:Retrospective Studies; D014412:Tumor Virus Infections / Q000150:complications / Q000473:pathology / Q000652:urine*
D017320:HIV Protease Inhibitors; D019469:Indinavir