Cloning and Preliminary Characterization of Regulatory Domain of Mouse Nestin Gene.
Mouse nestin genomic DNA(23.3 kb)was obtained after genomic library screening. Sequence comparison of the cDNA and genomic DNA revealed three introns in this gene. The positions and length of these introns are conserved among rat and human. The effect of the second intron of mouse nestin gene on the expression of luciferase reporter gene was investigated in vitro. Transient transfection of the second intron and its series deletions of mouse nestin gene showed that the second intron of mouse nestin gene could enhance reporter gene expression and the activity was contributed by a fragment about 800 bp long of its 3' end.
Cheng
Le-Ping
LP
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. njing@server.shcnc.ac.cn
Yang
Jing
J
Jing
Nai-He
NH