Bax expression protects yeast plasma membrane against ethanol-induced permeabilization.
The mechanism by which the expression of pro-apoptotic protein Bax is able to kill yeast was investigated. Ethanol stress induces a permeabilization of the plasma membrane revealed by propidium iodide accumulation. Bax expression, although killing yeast cells, prevents this permeabilization. These effects are modulated by aeration, by manipulation of the unsaturation index of fatty acids and by addition of resveratrol, a known inhibitor of lipid oxidation. These data suggest that lipid oxidation is involved in Bax effects. Taken together, these data show for the first time a direct effect of Bax on plasma membrane permeability properties and suggest that yeast is a powerful tool for investigating the molecular mechanisms underlying this process.
Marza
Esther
E
UMR5095 C.N.R.S./Université de Bordeaux 2, France.
Camougrand
Nadine
N
Manon
Stéphen
S
D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D002463:Cell Membrane Permeability; D004051:Diethylhexyl Phthalate / Q000378:metabolism / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D015870:Gene Expression; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D051028:bcl-2-Associated X Protein
D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D000431:Ethanol; D004051:Diethylhexyl Phthalate