Differential localisation of nPKC delta during cell cycle progression.
nPKC delta is a phospholipid-dependent and calcium-independent PKC isoform, whose over expression in BL6T murine melanoma cells, modifies their proliferative and metastatic potential in vivo. We focus here on the possible relationship between the subcellular localisation of nPKC delta and distinct phase of the cell cycle. Our findings show a dynamic localisation of nPKC delta in dependence of the phase of the cell cycle. Actually, this isoform is preferentially localised to the cytoplasm in serum-starved cells, shifting to the nucleus during the S-phase and becoming peri-nuclear, associated to the Golgi apparatus, in G2-M phase. Therefore, taken together our findings demonstrate that the subcellular localisation of nPKC delta changes dynamically during the cell cycle in dependence of the requirement of the enzyme at a particular place of the cell.
Perego
Carla
C
CNR, Institute of Neuroscience, Section of Cellular and Molecular Pharmacology and Department of Medical Pharmacology, University of Milan, Via Vanvitelli 32, 20129 Milano, Italy. c.perego@csfic.mi.cnr.it
Porro
Danilo
D
La Porta
Caterina A M
CA
D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D006056:Golgi Apparatus / Q000201:enzymology; D007527:Isoenzymes / Q000502:physiology*; D008545:Melanoma / Q000201:enzymology / Q000473:pathology; D051379:Mice; D011493:Protein Kinase C / Q000502:physiology*; D051745:Protein Kinase C-delta; D016196:S Phase; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; C497259:Prkcd protein, mouse; D011493:Protein Kinase C; D051745:Protein Kinase C-delta