Syndecan-2 mediates adhesion and proliferation of colon carcinoma cells.
Syndecan-2 is a transmembrane heparan sulfate proteoglycan whose function at the cell surface is unclear. In this study, we examined the function of syndecan-2 in colon cancer cell lines. In several colon cancer cell lines, syndecan-2 was highly expressed compared with normal cell lines. In contrast, syndecan-1 and -4 were decreased. Cell biological studies using the extracellular domain of recombinant syndecan-2 (2E) or spreading assay with syndecan-2 antibody-coated plates showed that syndecan-2 mediated adhesion and cytoskeletal organization of colon cancer cells. This interaction was critical for the proliferation of colon carcinoma cells. Blocking with 2E or antisense syndecan-2 cDNA induced G(0)/G(1) cell cycle arrest with concomitantly increased expression of p21, p27, and p53. Furthermore, blocking of syndecan-2 through antisense syndecan-2 cDNA significantly reduced tumorigenic activity in colon carcinoma cells. Therefore, increased syndecan-2 expression appears to be a critical for colon carcinoma cell behavior, and syndecan-2 regulates tumorigenic activity through regulation of adhesion and proliferation in colon carcinoma cells.
Park
Haein
H
Department of Life Sciences, Division of Molecular Life Sciences and Center for Cell Signaling Research, Ewha Medical School, Ewha Women's University, Seoul 120-750 Korea.
Kim
Yeonhee
Y
Lim
Yangmi
Y
Han
Innoc
I
Oh
Eok-Soo
ES
D000230:Adenocarcinoma / Q000473:pathology; D001483:Base Sequence; D002277:Carcinoma / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology*; D002453:Cell Cycle; D002455:Cell Division / Q000502:physiology*; D003110:Colonic Neoplasms / Q000473:pathology*; D017931:DNA Primers; D005434:Flow Cytometry; D006801:Humans; D008562:Membrane Glycoproteins / Q000502:physiology*; D011509:Proteoglycans / Q000502:physiology*; D053669:Syndecan-2
D017931:DNA Primers; D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508245:SDC2 protein, human; D053669:Syndecan-2