Analysis of phosphate position in hexose monosaccharides using ion-molecule reactions and SORI-CID on an FT-ICR mass spectrometer.
Through the use of ion-molecule reactions and SORI-CID, the phosphate position in hexose phosphate monosaccharides has been determined in the negative ion mode. Trimethyl borate was used as a reagent gas and was found to react readily with the phosphorylated hexose monosaccharides. After reaction of the reagent gas with the hexose phosphate, ion activation of the precursor by SORI-CID yielded different MS/MS spectra. Different diagnostic ions were generated for the two isomers, thus enabling differentiation and linkage position determination of the phosphate moiety.
Leavell
Michael D
MD
University of California, Berkeley 94720, USA.
Kruppa
Gary H
GH
Leary
Julie A
JA
D005583:Fourier Analysis; D005958:Glucosephosphates / Q000032:analysis; D006601:Hexoses / Q000032:analysis*; D013058:Mass Spectrometry; D009005:Monosaccharides / Q000032:analysis*
D005958:Glucosephosphates; D006601:Hexoses; D009005:Monosaccharides