YlALK1 encoding the cytochrome P450ALK1 in Yarrowia lipolytica is transcriptionally induced by n-alkane through two distinct cis-elements on its promoter.
The YlALK1 gene, which encodes cytochrome P450ALK1, plays a primary role in the assimilation of n-decane by yeast Yarrowia lipolytica and is inducible by n-decane at the transcriptional level. Deletion analysis of the YlALK1 promoter revealed that a 95-bp region on the YlALK1 promoter (from the position -400 to -304 upstream of the ATG codon) is essential for the induction by n-decane and we named this region ARR1 (alkane-responsive region). ARR1 was found to be made up of two different elements, ARE1 (alkane-responsive element 1; from -394 to -371) and ARE2 (from -325 to -305). By electrophoretic mobility shift assay, we found that the respective elements gave specific shift bands with the extracts from Y. lipolytica cells grown on n-alkane, but not much evidently from the cells grown on glycerol or glucose. This suggests that proteins that specifically bind to these elements are present and their binding or synthesis is dependent on n-alkane.
Sumita
Toru
T
Department of Biotechnology, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Iida
Toshiya
T
Yamagami
Setsu
S
Horiuchi
Hiroyuki
H
Takagi
Masamichi
M
Ohta
Akinori
A
D000473:Alkanes / Q000494:pharmacology*; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020218:Response Elements; D015533:Transcriptional Activation; D025062:Yarrowia / Q000201:enzymology*
D000473:Alkanes; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C116330:CYP52A3 protein, Candida maltosa; C012867:decane