title: Cytotoxic effects of denture base materials on a permanent human oral epithelial cell line and on primary human oral fibroblasts in vitro.
abstract: OBJECTIVE
  The objective of this study was to determine the cytocompatibility of three different extracts of denture base resins and to compare the cytotoxic effect of these materials on a human oral epithelial KB cell line and primary human oral fibroblasts derived from buccal mucosa.
  
  
  METHODS
  Set specimens from a heat-cured resin, a self-cured resin, and a light-cured resin were eluted with culture medium for 1, 3, and 5 days. Cytotoxicity was judged using tetrazolium bromide reduction assay.
  
  
  RESULTS
  The eluates from self-cured, heat-cured, and light-cured denture base resins were cytotoxic to primary human buccal fibroblast cultures and KB cells. Self-cured resin was the most toxic denture base material among the chemicals tested in all cultures. The cytotoxicity decreased in the order of self-cured resin > heat-cured resin > light-cured resin for KB cells. The rank for buccal fibroblast cells was self-cured resin > heat-cured resin > light-cured resin.
  
  
  CONCLUSIONS
  The influence of the cytotoxicity depended on the materials tested and the cell culture system used. The use of both permanent and primary cells is recommended for a better screening of the cytotoxic effects of denture base resins.
authors.affiliation: Department of Prosthodontics, Chung Shan Medical and Dental College Hospital, Taichung, Taiwan, Republic of China.
authors.forename: F M
authors.initials: FM
authors.lastname: Huang
authors.forename: K W
authors.initials: KW
authors.lastname: Tai
authors.forename: C C
authors.initials: CC
authors.lastname: Hu
authors.forename: Y C
authors.initials: YC
authors.lastname: Chang
mesh_terms: D000180:Acrylic Resins / Q000633:toxicity*; D000704:Analysis of Variance; D002460:Cell Line; D002478:Cells, Cultured; D004396:Coloring Agents; D003470:Culture Media; D003764:Dental Materials / Q000633:toxicity*; D003819:Denture Bases; D004847:Epithelial Cells / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007624:KB Cells / Q000187:drug effects; D009061:Mouth Mucosa / Q000187:drug effects* / Q000473:pathology; D015203:Reproducibility of Results; D013223:Statistics as Topic; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000180:Acrylic Resins; D004396:Coloring Agents; D003470:Culture Media; D003764:Dental Materials; D013778:Tetrazolium Salts; D013844:Thiazoles; C060386:Triad resin; C040291:Lucitone; C022616:thiazolyl blue