title: Distal regulatory regions restrict the expression of cis-linked genes to the tapetal cells.
abstract: The oleosin glycine-rich protein genes Atgrp-6, Atgrp-7, and Atgrp-8 occur in clusters in the Arabidopsis genome and are expressed specifically in the tapetum cells. The cis-regulatory regions involved in the tissue-specific gene expression were investigated by fusing different segments of the gene cluster to the uidA reporter gene. Common distal regulatory regions were identified that coordinate expression of the sequential genes. At least two of these genes were regulated spatially by proximal and distal sequences. The cis-acting elements (122 bp upstream of the transcriptional start point) drive the uidA expression to floral tissues, whereas distal 5' upstream regions restrict the gene activity to tapetal cells.
authors.affiliation: Laboratório de Genética Molecular Vegetal, Departamento de Genética, Universidade Federal do Rio de Janeiro, C.P. 68011, Rio de Janeiro, Brazil.
authors.forename: Luciana O
authors.initials: LO
authors.lastname: Franco
authors.forename: Carmem Lara
authors.initials: CL
authors.lastname: de O  Manes
authors.forename: Said
authors.initials: S
authors.lastname: Hamdi
authors.forename: Gilberto
authors.initials: G
authors.lastname: Sachetto-Martins
authors.forename: Dulce E
authors.initials: DE
authors.lastname: de Oliveira
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D001937:Brassica / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005810:Multigene Family; D009928:Organ Specificity; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D016601:RNA-Binding Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; D016601:RNA-Binding Proteins; C118496:GRP7 protein, Arabidopsis; C079353:oleosin protein, Arabidopsis