Influence of reduced intracellular glutathione availability on the secretion of vasoactive substances by human umbilical vein endothelial cells.
OBJECTIVE
Oxidative stress might be the reason for endothelial dysfunction in preeclampsia. The glutathione-peroxidase system is one of the primary antioxidants in the endothelium. We tested the effect of oxidative stress by reduction of glutathione availability on the secretion of vasoactive substances in endothelial cells.


METHODS
Endothelial cells in culture were incubated with different concentrations of buthionine-[S,R]-sulfoximine (BSO) or 1-chloro-2,4-dinitrobenzene (CDNB), both leading to a reduced intracellular availability of glutathione. The secretion of the vasoactive substances nitric oxide (NO), endothelin-I (ET-1), and prostacyclin (PGI2) was measured with respect to vitality and proliferation rate of the endothelial cells in culture.


METHODS
Effect of oxidative stress on the secretion of vasoactive substances from endothelial cells.


RESULTS
The oxidants CDNB and BSO have (in concentrations before evidence of cytotoxicity) a stimulating effect on the production of PGI2, they inhibit NO availability, and they do not significantly interfere with ET-1 production. Conclusion Oxidative stress in vitro induces an imbalance in the secretion of NO, ET-1, and PGI2 in endothelial cells.
Beinder
E
E
University Hospital of Erlangen/Nuremberg, Department of Obstetrics, Erlangen, Germany. ernst.beinder@gyn.med.uni-erlangen.de
Scalera
F
F
Schlembach
D
D
D001682:Biological Availability; D002478:Cells, Cultured; D019332:Endothelin-1 / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism*; D011464:Epoprostenol / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress; D011225:Pre-Eclampsia / Q000503:physiopathology*; D011247:Pregnancy; D014471:Umbilical Veins / Q000378:metabolism*
D019332:Endothelin-1; D009569:Nitric Oxide; D011464:Epoprostenol; D005978:Glutathione