Sucrose metabolism: Anabaena sucrose-phosphate synthase and sucrose-phosphate phosphatase define minimal functional domains shuffled during evolution.
Based on the functional characterization of sucrose biosynthesis related protiens[SBP: sucrose-phosphate synthase (SPS), sucrose-phosphate phosphatase (SPP), and sucrose synthase (SuS)] in Anabaena sp. PCC7120 and sequence analysis, we have shown that SBP are restricted to cyanobacterium species and plants, and that they are multidomain proteins with modular architecture. Anabaena SPS, a minimal catalytic SPS unit, defines a glucosyltransferase domain present in all SPSs and SuSs. Similarly, Anabaena SPP defines a phosphohydrolase domain characteristic of all SPPs and some SPSs. Phylogenetic analysis points towards the evolution of modern cyanobacterial and plant SBP from a bidomainal common ancestral SPS-like gene.
Cumino
Andrea
A
Centro de Investigaciones Biológicas, FIBA, Mar del Plata, Argentina.
Curatti
Leonardo
L
Giarrocco
Laura
L
Salerno
Graciela L
GL
D017033:Anabaena / Q000201:enzymology*; D000458:Cyanobacteria / Q000201:enzymology; D019143:Evolution, Molecular; D005964:Glucosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry; D010802:Phylogeny; D010944:Plants / Q000201:enzymology; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013395:Sucrose / Q000378:metabolism*
D013395:Sucrose; D005964:Glucosyltransferases; C022699:sucrose synthase; C022698:sucrose-phosphate synthase; C083874:sucrose-phosphate synthase phosphatase; D010749:Phosphoprotein Phosphatases; D010744:Phosphoric Monoester Hydrolases; C022697:sucrose-phosphatase