title: Genes coding structural proteins in the Leishmania braziliensis complex.
abstract: Acidic ribosomal P1 and P2b proteins, referred to as P proteins, and histone H3 are reported for first time in the Leishmania braziliensis complex. Deoxyribonucleic acid analysis and multiple sequence alignment suggest that both P proteins may maintain their structural function in the ribosomal stalk, in spite of the high rate of mutations detected. The deduced amino acid sequence of protein P1 showed 51% identity with Trypanosoma cruzi protein P1 and protein P2b showed 61% identity with T. cruzi protein P2b. Another conserved protein, L. (Viannia) braziliensis histone H3, showed 82% and 70% identity with histone H3 of L. (Leishmania) infantum and T. cruzi, respectively. The N-terminal end of this histone is divergent in comparison with the consensus eukaryotic sequence. Their predicted tridimensional structure was designed.
authors.affiliation: Instituto Nacional de Salud, Centro Nacional de Laboratorios de Salud Pública, Jr Capac Yupanqui 1400, Jesus Maria, Lima, Perú.
authors.forename: C
authors.initials: C
authors.lastname: Padilla
authors.forename: T
authors.initials: T
authors.lastname: Barreto
authors.forename: M
authors.initials: M
authors.lastname: De Los Santos
authors.forename: D C
authors.initials: DC
authors.lastname: Barker
authors.forename: C
authors.initials: C
authors.lastname: Carrillo
authors.forename: Y
authors.initials: Y
authors.lastname: Montoya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D016054:DNA, Protozoan / Q000235:genetics; D017125:Genes, Protozoan; D006657:Histones / Q000235:genetics; D007892:Leishmania braziliensis / Q000235:genetics*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics
chemical_list: D016054:DNA, Protozoan; D006657:Histones; D010750:Phosphoproteins; D015800:Protozoan Proteins; D012269:Ribosomal Proteins; C031937:phosphoprotein P2, ribosomal; C035690:ribosomal phosphoprotein P1