Comparison of several methods used for the determination of cephalosporins. Analysis of cephalexin in pharmaceutical samples.
The precision of UV absorbance of intact and acid degraded cephalosporins, ninhydrin, high performance liquid chromatography and iodometric methods used for analysis of cefoxitin, cefotaxime, cephazolin and cephalexin were compared. To obtain the calibration graphs the analytical signal used were: absorbance, first derivative absorbance, second derivative absorbance and H-point Standard Additions Method by using absorbance values at two selected wavelengths as analytical signal. These methods and calibration graphs were also used for the determination of cephalexin in pharmaceutical samples.
Gallo Martínez
Luisa
L
Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, C/Dr. Moliner 50, 46100 Burjasot, Valencia, Spain.
Campíns Falcó
Pilar
P
Sevillano Cabeza
Adela
A
D002214:Capsules; D002506:Cephalexin / Q000032:analysis*; D002511:Cephalosporins / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D009555:Ninhydrin; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D017186:Titrimetry
D002214:Capsules; D002511:Cephalosporins; D007202:Indicators and Reagents; D012996:Solutions; D009555:Ninhydrin; D002506:Cephalexin