title: Superoxide dismutase during glucose repression of Hansenula polymorpha CBS 4732.
abstract: Hansenula polymorpha CBS 4732 was studied during cultivation on methanol and different glucose concentrations. Activities of Cu/Zn and Mn superoxide dismutase, catalase and methanol oxidase were investigated. During cultivation on methanol, increased superoxide dismutase and catalase activities and an induced methanol oxidase were achieved. Transfer of a methanol grown culture to medium with a high glucose concentration caused growth inhibition, low consumption of carbon, nitrogen and phosphate substrates, methanol oxidase inactivation as well as decrease of catalase activity (21.8 +/- 0.61 deltaE240 x min(-1) x mg protein(-1)). At the same time, a high value for superoxide dismutase enzyme was found (42.9 +/- 0.98 U x mg protein(-1), 25% of which was represented by Mn superoxide dismutase and 75% - by the Cu/Zn type). During derepression methanol oxidase was negligible (0.005 +/- 0.0001 U x mg protein(-1)), catalase tended to be the same as in the repressed culture, while superoxide dismutase activity increased considerably (63.67 +/- 1.72 U x mg protein(-1), 69% belonging to the Cu/Zn containing enzyme). Apparently, the cycle of growth inhibition and reactivation of Hansenula polymorpha CBS 4732 cells is strongly connected with the activity of the enzyme superoxide dismutase.
authors.affiliation: Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
authors.forename: Tsonka
authors.initials: T
authors.lastname: Hristozova
authors.forename: Tanya
authors.initials: T
authors.lastname: Rasheva
authors.forename: Trayana
authors.initials: T
authors.lastname: Nedeva
authors.forename: Anna
authors.initials: A
authors.lastname: Kujumdzieva
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002474:Cell-Free System; D005947:Glucose / Q000494:pharmacology*; D007700:Kinetics; D000432:Methanol / Q000494:pharmacology; D010843:Pichia / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000429:Alcohol Oxidoreductases; C029293:methanol oxidase; D002374:Catalase; D013482:Superoxide Dismutase; D005947:Glucose; D000432:Methanol