Intracellular localization of the particulate methane monooxygenase and methanol dehydrogenase in Methylomicrobium album BG8.
The methanotrophic bacterium Methylomicrobium album BG8 uses methane as a sole source of carbon and energy. This bacterium forms an extensive intracytoplasmic membrane. The first enzymes of the methane oxidation pathway are the membrane-bound particulate methane monooxygenase and the periplasmic methanol dehydrogenase. Immunoelectron microscopy with specific antibodies was used to localize these enzymes to the intracytoplasmic membrane.
Brantner
Christine A
CA
Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, WI 53201, USA.
Remsen
Charles C
CC
Owen
Heather A
HA
Buchholz
Lorie A
LA
Perille Collins
Mary Lynne
ML
D000429:Alcohol Oxidoreductases / Q000032:analysis*; D000918:Antibody Specificity; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000201:enzymology / Q000648:ultrastructure; D008565:Membrane Proteins; D008772:Methylococcaceae / Q000201:enzymology*; D016253:Microscopy, Immunoelectron / Q000379:methods; D010105:Oxygenases / Q000032:analysis* / Q000737:chemistry
D008565:Membrane Proteins; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); D010105:Oxygenases; C021555:methane monooxygenase