Preliminary evaluation of mast cells and angiogenesis processes in experimental fibrosarcoma.
The aim of the study was the evaluation of angiogenesis processes in fibrosarcoma induced by 3-methylcholanthrene (3-Mc) in reference to the number of mast cells (MCs). 76 male Wistar rats were divided into 4 groups: two experimental (E) groups--after injection of 0.2 mg 3-Mc dissolved in olive oil (0.25ml), and 2 control (C) groups. In E1 group, 52 rats were killed after development of the fibrosarcoma; E(2)--10 rats were killed before development of the tumor; C(1)-8 rats received 0.25ml olive oil; C(2)--8 rats received no treatment. Tissue material was fixed in buffered formalin or Carnoy's and Bouin's fluid. Paraffin sections were stained with H+E and Azan methods, and with alcian blue-saphranine and toluidine blue. Immunohistochemical reactions detecting tryptase in MCs were also performed. Angiogenic objects (microvessels and single endothelial cells) were recognized using antibodies against factor VIII (vWF), P selectin (CD-62P), and CD-90. We found a distinct relationship between intensification of neoangiogenesis at the tumor periphery and increased number of MCs.
Sawicki
B
B
Department of Histology and Embryology, Medical Academy, Białystok, Poland. sawboghe@amb.edu.pl
Kasacka
I
I
Chyczewski
L
L
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002273:Carcinogens; D005354:Fibrosarcoma / Q000098:blood supply / Q000139:chemically induced / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000473:pathology*; D008748:Methylcholanthrene; D009389:Neovascularization, Pathologic / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000502:physiology; D012697:Serine Endopeptidases / Q000378:metabolism; D013194:Staining and Labeling; D016610:Tissue Embedding; D053802:Tryptases
D014408:Biomarkers, Tumor; D002273:Carcinogens; D008748:Methylcholanthrene; D012697:Serine Endopeptidases; C509223:Tpsab1 protein, rat; C509221:Tpsb2 protein, rat; D053802:Tryptases