title: Evaluation of cross-reactivity of allergens by use of intradermal testing in atopic dogs.
abstract: OBJECTIVE
  To examine cross-reactivity of aeroallergens in Colorado and surrounding states by evaluating concurrent positive reactions of related and nonrelated allergens of intradermal tests in dogs.
  
  
  METHODS
  Intradermal test results of 268 atopic dogs.
  
  
  METHODS
  A retrospective evaluation of skin test results for 268 dogs was performed. Pairs of closely related and nonrelated allergens were evaluated. Group 1 consisted of closely related allergens with demonstrated antibody cross-reactivity in humans. In group 2, allergens of the same plant group (ie, trees, grasses, or weeds) that were not closely related were paired. In group 3, allergen pairs were of different plant groups. Plant allergens were paired with dust mite allergens, animal dander, or mold spores in group 4. In the last group, allergens not derived from plants were paired. Data were evaluated twice by use of a different definition of a positive reaction. Significance of the difference between group means of log odds ratios was estimated by use of a boot-strap percentile confidence interval.
  
  
  RESULTS
  Significant differences in the number of concurrent positive reactions were not found between related versus nonrelated grass, weed, or tree allergens. Significant differences in the number of concurrent positive reactions were found between plant allergens of different groups (ie, grasses, weeds, and trees) and plant allergens of the same groups, related or nonrelated, as well as between plant-derived and nonplant-derived allergens. Many dogs reacting to a specific allergen did not react to a closely related allergen at the same time.
  
  
  CONCLUSIONS
  These results provide evidence against clinically relevant cross-reactivity and suggest that allergen-specific immunotherapy should be formulated on the basis of single allergen test results.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
authors.forename: Ralf S
authors.initials: RS
authors.lastname: Mueller
authors.forename: Phillip L
authors.initials: PL
authors.lastname: Chapman
authors.forename: Rodney E W
authors.initials: RE
authors.lastname: Rosychuk
authors.forename: Sonya V
authors.initials: SV
authors.lastname: Bettenay
authors.forename: Kathryn V
authors.initials: KV
authors.lastname: Fieseler
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D003120:Colorado; D016001:Confidence Intervals; D003429:Cross Reactions; D003876:Dermatitis, Atopic / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D007073:Immunoglobulin E / Q000276:immunology; D016017:Odds Ratio; D012189:Retrospective Studies; D012882:Skin Tests / Q000662:veterinary
chemical_list: D000485:Allergens; D007073:Immunoglobulin E