[Superoxide dismutase and catalase activities in carotenoid-synthesizing fungi Blakeslea trispora and Neurospora crassa under the oxidative stress].
The addition of menadione into the medium during cultivation of Neurospora crassa in the dark activated its constitutive superoxide dismutase. Exposure to light not only activated superoxide dismutase and catalase, but also increased the content of neurosporaxanthin. Superoxide dismutase activity in the mixed (+/-) mycelium of Blakeslea trispora synthesizing beta-carotene in the dark was much lower than that in Neurospora crassa. The superoxide dismutase activity further decreased in oxidative stress. The catalase activity decreased with an increase in the content of beta-carotene. Our results indicate that neurosporaxanthin possesses photoprotective properties in Neurospora crassa. In Blakeslea trispora (+/-) fungi, this compound acts as a major antioxidant during inactivation of enzymes that detoxify reactive oxygen species.
Gessler
N N
NN
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071 Russia.
Sokolov
A V
AV
Bykhovskiĭ
V Ia
VIa
Belozerskaia
T A
TA
D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism*; D003470:Culture Media; D005658:Fungi / Q000201:enzymology / Q000378:metabolism*; D008027:Light; D009492:Neurospora crassa / Q000378:metabolism; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism*; D024483:Vitamin K 3; D019820:Xanthine / Q000378:metabolism; D019207:beta Carotene / Q000096:biosynthesis
D000975:Antioxidants; D003470:Culture Media; D019207:beta Carotene; D019820:Xanthine; D024483:Vitamin K 3; D002374:Catalase; D013482:Superoxide Dismutase
Aktivnost' superoksiddismutazy i katalazy u karotinoidsinteziruiushchikh gribov Blakeslea trispora i Neurospora crassa v usloviiakh okislitel'nogo stressa.