Glycosidases during chick embryo lung development and their colocalization with proteoglycans and growth factors.
During development, the epithelial component of the lung goes through a complex orderly process of branching, following strict patterns of space and time. Proteoglycans, glycosaminoglycans and growth factors are fundamental components of the extracellular matrix and perform a key role in differentiative processes. The embryonic chick lung shows a specific glycosaminoglycan composition at different levels of branching and at different embryonic stages. Proteoglycan and glycosaminoglycan accumulation is the result of secretion, absorption and degradation processes. In this pathway, enzymes, such as glycosidases, growth factors and cytokines are involved. We examined the behaviour of glycosidases, such as beta-hexosaminidases (beta-N-acetyl-D-glucosaminidase, beta-N-acetyl-D-galactosaminidase), beta-glucuronidase and beta-galactosidase, during the development of the lung bud. Our data show that the activity of the enzymes is closely linked to the processes of epithelial proliferation, bronchial tubule lengthening and infiltration of the surrounding mesenchyme. The glycosaminoglycans colocalize with transforming growth factor beta2 and interleukin-1 in the basement membrane and in the mesenchymal areas where the epithelium grows, and are complementary to the presence of the glycosidases. In conclusion, the activity of these glycosidases is spatially and temporally programmed and favors the release of the factors and the events which they influence.
Stabellini
G
G
Dipartimento di Anatomia Umana, Università Statale di Milano, Italy. giordano.stabellini@unimi.it
Calvitti
M
M
Baroni
T
T
Marinucci
L
L
Calastrini
C
C
Carinci
P
P
Becchetti
E
E
D000818:Animals; D001485:Basement Membrane / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005347:Fibroblasts / Q000737:chemistry / Q000201:enzymology; D019084:Fluorescent Antibody Technique, Indirect; D006025:Glycosaminoglycans / Q000032:analysis / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000032:analysis / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000032:analysis / Q000378:metabolism*; D008168:Lung / Q000737:chemistry / Q000196:embryology / Q000201:enzymology*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000378:metabolism*; D053781:Transforming Growth Factor beta2
D006025:Glycosaminoglycans; D007375:Interleukin-1; D016212:Transforming Growth Factor beta; D053781:Transforming Growth Factor beta2; D006026:Glycoside Hydrolases