Studies on the signal cascade mechanism mediating the cardioprotective actions of bradykinin.
The cardioprotective involvement of bradykinin was evaluated using the ACE inhibitor, lisinopril, and the APP inhibitor, 2-mercaptoethanol alone and in combination in rats with experimental myocardial infarction. The signal cascade mechanism mediating the cardioprotective actions of bradykinin was evaluated by administering aspirin and methylene blue prior to lisinopril and 2-mercaptoethanol combined treatment. Myocardial infarction was produced by occlusion of the left anterior descending coronary artery for 30 min followed by 4 h of reperfusion. Infarct size was measured by the TTC stain method. Serum free radical levels were estimated by the method developed by Yagi. A lead II electrocardiogram was monitored throughout the experiment. With the combined inhibition of both the enzymes ACE and APP, a better cardioprotection was observed. The observed cardioprotection was decreased with the prior administration of aspirin and methylene blue. The results suggest the cardioprotective role of bradykinin during experimental myocardial infarction. The results are further suggesting the involvement of both prostaglandins and nitric oxide pathways in the cardioprotective actions of bradykinin.
Akula
A
A
Pharmacology Division, Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India.
Veeravalli
K K
KK
Potharaju
S
S
Kota
M K
MK
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology*; D005260:Female; D006352:Heart Ventricles / Q000473:pathology; D017706:Lisinopril / Q000494:pharmacology; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D008751:Methylene Blue / Q000494:pharmacology; D009203:Myocardial Infarction / Q000473:pathology / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000517:prevention & control; D009206:Myocardium / Q000473:pathology; D009336:Necrosis; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D011448:Prostaglandin Antagonists / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects*
D000806:Angiotensin-Converting Enzyme Inhibitors; D011448:Prostaglandin Antagonists; D009569:Nitric Oxide; D008623:Mercaptoethanol; D017706:Lisinopril; D001241:Aspirin; D001920:Bradykinin; D008751:Methylene Blue