XSPR-1 and XSPR-2, novel Sp1 related zinc finger containing genes, are dynamically expressed during Xenopus embryogenesis.
Proteins related to the human transcription factor Sp1 are characterized by the presence of a highly conserved zinc finger domain consisting of three C2H2 type zinc fingers. Here we describe two Xenopus laevis cDNAs, which encode novel Sp1-related C2H2 type zinc finger transcription factors named XSPR-1 and XSPR-2. Structurally, XSPR-1 and XSPR-2 are closely related to the murine Sp5, which interacts genetically with Brachyury (Dev. Biol. 227 (2000) 358). XSPR-1 and XSPR-2 are expressed in broad and dynamic patterns during early development. Starting at gastrulation, XSPR-1 transcripts are restricted to the non-involuting marginal zone, and, at later stages, to the neuroectoderm, forebrain, otic vesicles and the midbrain/hindbrain boundary. In contrast, XSPR-2 expression is found predominantly within the presumptive mesoderm during gastrulation. At tailbud and tadpole stages, XSPR-2 is expressed exclusively in the tip of the tail.
Ossipova
Olga
O
Georg-August-Universität Göttingen, Institute of Biochemistry and Molecular Cell Biology, Humboldtallee 23, D-37073 Göttingen, Germany. olga_ossipova@dfci.harvard.edu
Stick
Reimer
R
Pieler
Tomas
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D016329:Sp1 Transcription Factor / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D029867:Xenopus Proteins / Q000235:genetics*; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics*; D016335:Zinc Fingers
D018076:DNA, Complementary; C470295:SP5 protein, Xenopus; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D029867:Xenopus Proteins; C470294:sp5l protein, Xenopus