Overexpression of human metallothionein-III prevents hydrogen peroxide-induced oxidative stress in human fibroblasts.
Metallothioneins (MT) are ubiquitous low molecular weight metal binding proteins that may act as antioxidants. In the present study, the cloned human MT-III coding region was permanently transfected into GM00637 cells in order to investigate the antioxidative effects of this brain-specific MT isoform. GM00637/MT-III cells overexpressed MT-III mRNA versus pcDNA3 plasmid-transfected control cells (GM00637). When challenged with H(2)O(2), the GM00637/MT-III cells displayed significantly more resistance than the GM00637 cells, as determined by cell cytotoxicity, lactate dehydrogenase leakage, and lipid peroxidation. In addition, the GM00637/MT-III cells were highly protected from the H(2)O(2)-induced production of reactive oxygen species and DNA damage. These results directly support the antioxidative role of MT-III, and demonstrate MT-III can scavenge free oxygen radicals and protect cells from oxidative stress.
You
Ho Jin
HJ
Department of Pharmacology, School of Medicine, Chosun University, Kwangju, South Korea. hgjeong@mail.chosun.ac.kr
Lee
Kyung Jin
KJ
Jeong
Hye Gwang
HG
D000975:Antioxidants; D004249:DNA Damage; D005347:Fibroblasts / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology* / Q000633:toxicity; D015227:Lipid Peroxidation; D008668:Metallothionein / Q000235:genetics*; D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins / Q000235:genetics*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species
D000975:Antioxidants; D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins; D017382:Reactive Oxygen Species; D008668:Metallothionein; D006861:Hydrogen Peroxide