Microwave-assisted rapid characterization of lipase selectivities.
A rapid screening procedure for characterization of lipase selectivities using microwaves was developed. The rate of reaction of various commercial lipases (porcine pancreas, Mucor miehei, Candida rugosa, Pseudomonas cepacia) as well as lipases from laboratory isolates-Bacillus stearothermophilus and Burkholderia cepacia RGP-10 for triolein hydrolysis was 7- to 12-fold higher in a microwave oven as compared to that by pH stat. The esterification of sucrose/methanol and ascorbic acid with different fatty acids was also achieved within 30 s in a microwave using porcine pancreas, B. stearothermophilus SB-1 and B. cepacia RGP-10 lipases. The relative rates and selectivity of the lipases both for hydrolytic and synthesis reactions remains unaltered. However, the rate of reaction was dynamically enhanced when exposed to microwaves. Microwave-assisted enzyme catalysis can become an attractive procedure for rapid characterization of large number of enzyme samples and substrates, which otherwise is a cumbersome and time-consuming exercise.
Bradoo
Sapna
S
Biotechnology Department, Center for Chemistry and Chemical Engineering, Lund University, Box 124, SE-22100, Lund, Sweden.
Rathi
Pooja
P
Saxena
R K
RK
Gupta
Rani
R
D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry; D001419:Bacteria / Q000201:enzymology*; D001671:Biochemistry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D008872:Microwaves; D013379:Substrate Specificity; D013395:Sucrose / Q000737:chemistry; D013552:Swine; D013997:Time Factors; D014280:Triglycerides / Q000737:chemistry
D014280:Triglycerides; D013395:Sucrose; D008049:Lipase; D001205:Ascorbic Acid