The Gef1 protein of Saccharomyces cerevisiae is associated with chloride channel activity.
The Gef1 protein of the yeast Saccharomyces cerevisiae (Gef1p) has amino acid homology to the voltage-gated CLC chloride channel family. It has been postulated that it provides the compensatory transport of Cl- anions to the lumen of the Golgi thereby regulating the pH of this compartment. Using GEF1 fusion with heterologous promoter we obtained a yeast strain highly overproducing Gef1p. The electrophysiological properties of the microsomal fraction obtained from this strain were measured using lipid bilayer system. Our data indicate that Gef1p is associated with the chloride channel activity. This anion-selective channel has a unitary conductance of 42 pS when measured in symmetrical 600/600 mM TEA-Cl solutions, is voltage-dependent, and closes at high negative voltages.
Flis
Krzysztof
K
Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, Poland.
Bednarczyk
Piotr
P
Hordejuk
Renata
R
Szewczyk
Adam
A
Berest
Vladimir
V
Dolowy
Krzysztof
K
Edelman
Aleksander
A
Kurlandzka
Anna
A
D018118:Chloride Channels / Q000235:genetics / Q000502:physiology*; D004553:Electric Conductivity; D008051:Lipid Bilayers; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D008861:Microsomes / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000032:analysis; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*
D018118:Chloride Channels; C089713:GEF1 protein, S cerevisiae; D008051:Lipid Bilayers; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins