[Proteomics in clinical enzymology: polymorphism of creatine kinase and alkaline phosphatase].
BACKGROUND
The clinical proteomics is dedicated to the use of the proteomics in the clinical laboratory science.


OBJECTIVE
Examples for the importance of clinical proteomics are provided by studies on the polymorphisms of creatine kinase and alkaline phosphatase enzymes.


METHODS
The different (immunoinhibition, electrophoretic, immunochemistry) assays for determination of a cardiac marker (creatine kinase 2) are compared in hospital patients. Isoform analysis of alkaline phosphatase is performed in benign, transient hyperphosphatasemia.


RESULTS
The author reviews recent knowledge on polymorphism of creatine kinase (isoenzymes, isoforms, macro types), and alkaline phosphatase (isoenzymes, their cancer related variants, isoforms). The origin of falsely high cardiac marker (creatine kinase 2) determined by the immunoinhibition assay could be related to the presence of macro creatine kinase (both types), creatine kinase isoenzyme 1, and their mixtures. After reviewing the recent findings on the syndrome of benign, transient hyperphosphatasemia, a case of a 1-year-old Hungarian boy with this syndrome is presented.


CONCLUSIONS
The author points out that the appropriate use of clinical proteomics (the polymorphisms of creatine kinase and alkaline phosphatase enzymes) may improve the diagnostic efficiency of the clinical laboratory tests.
Góth
László
L
Orvos- és Egészségtudományi Centrum, Altalános Orvostudományi Kar, Klinikai Biokémiai és Molekuláris Patológiai Intézet, Debreceni Egyetem, Debrecen.
D000469:Alkaline Phosphatase / Q000097:blood / Q000235:genetics*; D015415:Biomarkers / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D001921:Brain / Q000201:enzymology; D009202:Cardiomyopathies / Q000201:enzymology; D003402:Creatine Kinase / Q000097:blood / Q000235:genetics*; D004586:Electrophoresis; D006801:Humans; D007120:Immunochemistry; D007527:Isoenzymes; D008928:Mitochondria / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D009369:Neoplasms / Q000201:enzymology; D011110:Polymorphism, Genetic; D020543:Proteome / Q000235:genetics
D015415:Biomarkers; D014408:Biomarkers, Tumor; D007527:Isoenzymes; D020543:Proteome; D003402:Creatine Kinase; D000469:Alkaline Phosphatase
A fehérjekutatás a klinikai enzimológiai gyakorlatban: a kreatinkináz és alkalikus foszfatáz enzimek polimorfizmusa.