OASIS is a transcriptional activator of CREB/ATF family with a transmembrane domain.
Murine OASIS is a putative CREB/ATF family transcription factor that is induced in gliosis, but its molecular role has not been determined. We have isolated the human OASIS gene and investigated the potential of OASIS protein as a transcriptional activator. We found that OASIS can activate transcription through box-B elements but not through the somatostatin CRE. OASIS contains a putative C-terminal hydrophobic transmembrane domain, a typical structural feature for the transcription factors activated by regulated intramembrane proteolysis. Truncation of the OASIS transmembrane domain resulted in a significant increase in transcriptional activity and altered its subcellular localization from the endoplasmic reticulum to the nucleus. Western blot analysis of transfected cells identified OASIS polypeptides of 82 and 66 kDa. These results suggest that the transmembrane domain plays an important role in the regulation of transcriptional activation by OASIS.
Omori
Yoshihiro
Y
Laboratory of Genome Structure Analysis Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokane-dai, Minato-ku, Tokyo 108-8639, Japan. y_omori@research.otsuka.co.jp
Imai
Jun-ichi
J
Suzuki
Yutaka
Y
Watanabe
Shinya
S
Tanigami
Akira
A
Sugano
Sumio
S
D051696:Activating Transcription Factors; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D015152:Blotting, Northern; D019556:COS Cells; D002522:Chlorocebus aethiops; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D017931:DNA Primers; D020224:Expressed Sequence Tags; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D016296:Mutagenesis; D009419:Nerve Tissue Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D051696:Activating Transcription Factors; D001798:Blood Proteins; C485618:CREB3L1 protein, human; C119923:Creb3l1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D017931:DNA Primers; D009419:Nerve Tissue Proteins; D015534:Trans-Activators; D014157:Transcription Factors