title: Specific induction of apoptosis by 1,8-cineole in two human leukemia cell lines, but not a in human stomach cancer cell line.
abstract: We have investigated the effects of 1,8-cineole [the main component of essential oil prepared from bay-leaves Laurus nobilis L.)] on DNA of human leukemia cell lines, Molt 4B, HL-60 and stomach cancer KATO III cells. Specific induction of apoptosis by 1,8-cineole was observed in human leukemia Molt 4B and HL-60 cells, but not in human stomach cancer KATO III cells. Morphological changes showing apoptotic bodies were observed in the human leukemia HL-60 cells treated with 1,8-cineole. The fragmentations of DNA by cineole to oligonucleosomal-sized fragments that is a characteristic of apoptosis were concentration- and time-dependent in Molt 4B and HL-60 cells, but not in KATO III cells. The present study shows that the suppression growth by 1,8-cineole in the leukemia cell lines results from the induction of apoptosis by this compound.
authors.affiliation: Faculty of Bioresources, Mie University, Tsu-city, Mie 514-0001, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Moteki
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Hibasami
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Yamada
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Katsuzaki
authors.forename: Kunio
authors.initials: K
authors.lastname: Imai
authors.forename: Takashi
authors.initials: T
authors.lastname: Komiya
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D003511:Cyclohexanols / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000187:drug effects*; D000077591:Eucalyptol; D006801:Humans; D027442:Laurus / Q000737:chemistry; D007938:Leukemia / Q000473:pathology*; D039821:Monoterpenes; D013274:Stomach Neoplasms / Q000473:pathology*; D013729:Terpenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D003511:Cyclohexanols; D004273:DNA, Neoplasm; D039821:Monoterpenes; D013729:Terpenes; D000077591:Eucalyptol