Assessing probability of ancestry using simple sequence repeat profiles: applications to maize hybrids and inbreds.
Determination of parentage is fundamental to the study of biology and to applications such as the identification of pedigrees. Limitations to studies of parentage have stemmed from the use of an insufficient number of hypervariable loci and mismatches of alleles that can be caused by mutation or by laboratory error and that can generate false exclusions. Furthermore, most studies of parentage have been limited to comparisons of small numbers of specific parent-progeny triplets thereby precluding large-scale surveys of candidates where there may be no prior knowledge of parentage. We present an algorithm that can determine probability of parentage in circumstances where there is no prior knowledge of pedigree and that is robust in the face of missing data or mistyped data. We present data from 54 maize hybrids and 586 maize inbreds that were profiled using 195 SSR loci including simulations of additional levels of missing and mistyped data to demonstrate the utility and flexibility of this algorithm.
Berry
Donald A
DA
Department of Biostatistics, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030-4009, USA.
Seltzer
Jon D
JD
Xie
Chongqing
C
Wright
Deanne L
DL
Smith
J Stephen C
JS
D000465:Algorithms; D001947:Breeding; D003627:Data Interpretation, Statistical; D006824:Hybridization, Genetic; D018598:Minisatellite Repeats; D010802:Phylogeny; D003313:Zea mays / Q000235:genetics*