Identification and functional expression of HCx31.9, a novel gap junction gene.
By combining in silico and bench molecular biology methods we have identified a novel human gap junction gene that encodes a protein designated HCx31.9. We have determined its human chromosomal location and gene structure, and we have identified a putative mouse ortholog, mCx30.2. We have observed the presence of HCx31.9 in human cerebral cortex, liver, heart, spleen, lung, and kidney and the presence of mCx30.2 in mouse cerebral cortex, liver and lung. Moreover, preliminary data on the electrophysiological properties of HCx31.9 have been obtained by functional expression in paired Xenopus oocytes and in transfected N2A cells.
Belluardo
N
N
Department of Experimental Medicine, University of Palermo, Italy.
White
T W
TW
Srinivas
M
M
Trovato-Salinaro
A
A
Ripps
H
H
Mudò
G
G
Bruzzone
R
R
Condorelli
D F
DF
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D017630:Connexins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D017629:Gap Junctions / Q000737:chemistry / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D018408:Patch-Clamp Techniques; D010802:Phylogeny; D016415:Sequence Alignment; D014018:Tissue Distribution; D014982:Xenopus laevis
D017630:Connexins; C468137:GJD3 protein, human