title: DNA fragmentation in V79 cells irradiated with light ions as measured by pulsed-field gel electrophoresis. I. Experimental results.
abstract: OBJECTIVE
  To compare the results on DNA fragmentation induced in Chinese hamster V79 cells by various doses of gamma-rays and low-energy protons and helium-4 ions.
  
  
  METHODS
  V79 cells were irradiated as monolayers with monoenergetic protons and helium-4 ions; gamma-rays were used as the reference radiation. DNA double-strand breaks were evaluated by calibrated pulsed-field gel electrophoresis using conditions covering the range 5.7 Mbp-23.1 kbp.
  
  
  RESULTS
  The fragment-counting method gave double-strand breaks yields and the relative biological effectiveness higher than those obtained by the fraction of activity released method. The frequency distribution of fragments showed that protons and helium ions induced more fragments below the Mbp region than did gamma-rays at the same dose. The distributions for both the irradiated and non-irradiated samples clearly appeared to be non-random.
  
  
  CONCLUSIONS
  Differences were observed in the yield and spatial correlation, at a molecular size scale characteristic of loop dimensions, of the double-strand breaks induced by gamma-rays and by light ions. These effects may have a role in the observed different cell response to these radiations.
authors.affiliation: Physics Laboratory, Istituto Superiore di Sanità, Rome, Italy. m.belli@iss.it
authors.forename: M
authors.initials: M
authors.lastname: Belli
authors.forename: R
authors.initials: R
authors.lastname: Cherubini
authors.forename: M
authors.initials: M
authors.lastname: Dalla Vecchia
authors.forename: V
authors.initials: V
authors.lastname: Dini
authors.forename: G
authors.initials: G
authors.lastname: Esposito
authors.forename: G
authors.initials: G
authors.lastname: Moschini
authors.forename: O
authors.initials: O
authors.lastname: Sapora
authors.forename: G
authors.initials: G
authors.lastname: Simone
authors.forename: M A
authors.initials: MA
authors.lastname: Tabocchini
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000302:isolation & purification / Q000528:radiation effects*; D004249:DNA Damage; D053938:DNA Fragmentation / Q000528:radiation effects*; D016521:Electrophoresis, Gel, Pulsed-Field; D005720:Gamma Rays; D006371:Helium; D011522:Protons
chemical_list: D011522:Protons; D006371:Helium; D004247:DNA