Detecting large biomolecules from high-salt solutions by fused-droplet electrospray ionization mass spectrometry.
A novel fused-droplet electrospray ionization (FD-ESI) source was developed to generate peptide and protein ions. The sample solution was first ultrasonically nebulized to form fine aerosols. The aerosols were then purged into a glass reaction chamber via nitrogen. Charged methanol droplets were continuously generated through electrospraying the acidic methanol solution from a capillary, which was located at the center of the reaction chamber. As the sample aerosols entered the reaction chamber, they fused with the charged methanol droplets from which electrospray proceeded continuously. The mass spectra of peptide and protein that FD-ESI-MS produced were practically identical to those that conventional ESI-MS produced. However, FD-ESI-MS resulted in an extremely high salt tolerance. Cytochrome c ions were detected in the solutions that contained 10% (w/w; 1.709 M) NaCl or 2.5% (425 mM) NaH2PO4. As with those obtained from the solution that lacked NaCl and NaH2PO4, the width of cytochrome c ion peaks remained nearly unchanged.
Chang
Der-Yeou
DY
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan.
Lee
Chia-Cheng
CC
Shiea
Jentaie
J
D001920:Bradykinin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003574:Cytochrome c Group / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012965:Sodium Chloride; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D003574:Cytochrome c Group; D010455:Peptides; D011506:Proteins; D012965:Sodium Chloride; D001920:Bradykinin