title: B cell receptor- and beta 2-adrenergic receptor-induced regulation of B7-2 (CD86) expression in B cells.
abstract: The costimulatory molecule B7-2 (CD86) is expressed on the surface of APCs, including B cells. Considering the importance of B7-2 in regulating both T and B cell function, it may be important to understand the regulatory mechanisms governing its expression. We report in this study that stimulation of the B cell receptor (BCR) and/or a neurotransmitter receptor, the beta(2)-adrenergic receptor (beta(2)AR), may cooperate to regulate B cell-associated B7-2 expression in vitro and in vivo. beta(2)AR stimulation further enhanced the level of BCR-induced B7-2 expression in B cells potentially via protein tyrosine kinase-, protein kinase A-, protein kinase C-, and mitogen-activated protein kinase-dependent mechanisms. Importantly, BCR and/or beta(2)AR stimulation, but not histone hyperacetylation and DNA hypomethylation alone, increased B cell-associated B7-2 expression by increasing B7-2 mRNA stability, NF-kappa B nuclear binding, and NF-kappa B-dependent gene transcription. Thus, this study provides additional insight into the signaling intermediates and molecular mechanisms by which stimulation of the BCR and beta(2)AR may regulate B cell-associated B7-2 expression.
authors.affiliation: Department of Cell Biology, Loyola University Stritch School of Medicine, Maywood, IL 60153, USA.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Kohm
authors.forename: Afsaneh
authors.initials: A
authors.lastname: Mozaffarian
authors.forename: Virginia M
authors.initials: VM
authors.lastname: Sanders
mesh_terms: D000107:Acetylation; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D001402:B-Lymphocytes / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism* / Q000637:transplantation; D051940:B7-2 Antigen; D002478:Cells, Cultured; D019175:DNA Methylation; D005260:Female; D006657:Histones / Q000378:metabolism / Q000502:physiology; D007399:Interphase / Q000235:genetics / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016513:Mice, SCID; D009687:Nuclear Proteins / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism / Q000502:physiology; D011485:Protein Binding / Q000276:immunology; D011505:Protein-Tyrosine Kinases / Q000378:metabolism / Q000502:physiology; D020871:RNA Stability / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism / Q000502:physiology*; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D006657:Histones; D008562:Membrane Glycoproteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D018343:Receptors, Adrenergic, beta-2; D011947:Receptors, Antigen, B-Cell; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases; D010749:Phosphoprotein Phosphatases