Purified malaria pigment (hemozoin) enhances dendritic cell maturation and modulates the isotype of antibodies induced by a DNA vaccine.
Hemozoin (malaria pigment) has been implicated in the modulation of immune responses during malaria infection. This study was designed to evaluate the effect of purified hemozoin on the in vitro activation of myeloid dendritic cells. Our study also revealed that in addition to enhancing the maturation of dendritic cells, hemozoin also greatly promotes immunoglobulin G2a antibody responses when coadministered with a DNA vaccine plasmid encoding Pfs25, a Plasmodium falciparum transmission-blocking antigen.
Coban
Cevayir
C
Department of Molecular Microbiology and Immunology, Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA.
Ishii
Ken J
KJ
Sullivan
David J
DJ
Kumar
Nirbhay
N
D000276:Adjuvants, Immunologic; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D005260:Female; D006420:Hemeproteins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000096:biosynthesis; D008288:Malaria / Q000276:immunology / Q000517:prevention & control; D017780:Malaria Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D010860:Pigments, Biological; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D006420:Hemeproteins; D007132:Immunoglobulin Isotypes; D017780:Malaria Vaccines; C071284:Pfs25 protein, Plasmodium falciparum; D010860:Pigments, Biological; D015800:Protozoan Proteins; D019444:Vaccines, DNA; D018664:Interleukin-12; C020753:hemozoin