Application of alkaline elution, Fast Micromethod and flow cytometry in detection of marine contamination.
DNA damage is an inescapable aspect of life in the biosphere. The presented investigations were an attempt to examine the response of a DNA damage as a biomarker of environmental quality in the mussels Mytilus galloprovincialis sampled at differently contaminated areas of Istrian coast, Northern Adriatic. The investigations were performed in order to get information about the genotoxic risk for marine organisms exposed to mixed environmental pollution, as well as the information about the presence of unknown mixture of genotoxic contaminants in the marine environment. Types of DNA damage detected are alkali-labile sites and single-strand breaks measured by Fast Micromethod, interstrand cross-links and DNA protein cross-links by alkaline filter elution and cell cycle disturbation by flow cytometry. The applicability of all three methods for marine quality control is discussed.
Bihari
Nevenka
N
Institut Ruder Bosković, Center for Marine Research Rovinj, Croatia. bihari@cim.irb.hr
Hamer
Bojan
B
Jaksić
Zeljko
Z
Fafandel
Maja
M
Micić
Milena
M
Batel
Renato
R
D000818:Animals; D049872:Bivalvia / Q000235:genetics*; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D005374:Filtration; D005434:Flow Cytometry / Q000379:methods*; D009153:Mutagens / Q000032:analysis; D014877:Water Pollution, Chemical / Q000032:analysis*
D009153:Mutagens; D004247:DNA