The influence of storage time upon the real thickness of the histological brain sections.
The aim of the study was to assess the effect of storage time on the actual thickness of the histological sections. The study was performed on 5 brains of adult Wistar rats. The most rapid changes occur at the beginning of the storage process, after about one month the dynamics of changes decreases, but still the thickness of the sections diminishes. It is suggested that quantitative analyses should be performed in similar period of time and the critical care should be taken not only of the chemicals and procedures used, but also of the control of environmental factors.
Dziewiatkowski
J
J
Department of Anatomy and Neurobiology, Medical University, Gdańsk, Poland. jdziew@amg.gda.pl
Spodnik
J H
JH
Wójcik
S
S
Spodnik
E
E
Moryś
J
J
D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D004396:Coloring Agents; D005615:Freezing; D008867:Microtomy; D051381:Rats; D017208:Rats, Wistar; D013048:Specimen Handling; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation
D004396:Coloring Agents