Overexpression of VDUP1 mRNA sensitizes HeLa cells to paraquat.
5-Bromodeoxyuridine (BrdU) induces or suppresses senescence-associated genes in any types of mammalian cells. From a cDNA library upregulated by BrdU in HeLa cells, we identified the gene encoding VDUP1 as a senescence-associated gene in normal human fibroblasts. To address a role of VDUP1 in senescence, we established HeLa cell clones, V7 and V27, which express its mRNA in a doxycycline-dependent manner. Although their growth in liquid culture was moderately retarded, colony formation on semi-solid medium was strongly inhibited by overexpression of the mRNA. We also examined susceptibility of these clones to various reagents. Consequently, colony formation in liquid culture was strongly inhibited by paraquat in these clones. Their superoxide dismutase activity was normal.
Joguchi
Atsuhiro
A
Division of Biochemistry, Kihara Institute for Biological Research and Graduate School of Integrated Sciences, Yokohama City University, Maioka-cho 641-12, Totsuka-ku, Yokohama 244-0813, Japan.
Otsuka
Izumi
I
Minagawa
Sachi
S
Suzuki
Toshikazu
T
Fujii
Michihiko
M
Ayusawa
Dai
D
D001973:Bromodeoxyuridine / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics*; D002455:Cell Division / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology; D004318:Doxycycline / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D010269:Paraquat / Q000494:pharmacology*; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*; D013482:Superoxide Dismutase / Q000378:metabolism; D013879:Thioredoxins / Q000235:genetics*
D002352:Carrier Proteins; D012333:RNA, Messenger; C486715:TXNIP protein, human; D013879:Thioredoxins; D013482:Superoxide Dismutase; D001973:Bromodeoxyuridine; D004318:Doxycycline; D010269:Paraquat