Successive cultures of mature hepatocytes for hepatocyte autotransplantation to assist liver function after liver resection for cancer.
We developed a method for the rapid successive cultures of adult rat mature hepatocytes on plastic dishes while avoiding viral transformation or co-culture with other cell lines. This method also allows for culturing adult human mature hepatocytes up to the secondary culture. These can be expected to provide a good source for hepatocyte autotransplantation, and, combined with the previously reported methods for the transplantation of hepatocytes into the spleen, a promising option for the support of liver function after liver resection for cancer without the need for immunosuppressive agents.
Funaki
Naomi
N
Department of Surgery, Shiga Medical Center for Adults, Moriyama City, Shiga 524, Japan.
Tanaka
Junji
J
Sugiyama
Taketoshi
T
Ohshio
Gakuji
G
Nonaka
Atsushi
A
Yotsumoto
Fumiaki
F
Sugie
Tomoharu
T
Imamura
Masayuki
M
D000328:Adult; D000418:Albumins / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D017690:Cell Transplantation; D002478:Cells, Cultured / Q000166:cytology; D006003:Glycogen / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology* / Q000637:transplantation*; D006801:Humans; D007166:Immunosuppressive Agents; D008099:Liver / Q000502:physiology* / Q000601:surgery; D008113:Liver Neoplasms / Q000601:surgery*; D008297:Male; D008858:Microscopy, Phase-Contrast; D008938:Mitosis; D051381:Rats; D017208:Rats, Wistar; D014182:Transplantation, Autologous
D000418:Albumins; D007166:Immunosuppressive Agents; D006003:Glycogen