Addition of Polybrene improves stability of organophosphate hydrolase immobilized in poly(vinyl alcohol) cryogel carrier.
Organophosphate hydrolase, covalently attached to the beads of poly(vinyl alcohol) cryogel in the presence of Polybrene, was fivefold more stable in 15% (v/v) ethanol solution than the free enzyme. Immobilized biocatalyst, prepared with an addition of Polybrene, retained a half of its initial activity in 50% (v/v) aqueous ethanol solution, 90% of activity during 10 working cycles of Paraoxon hydrolysis and 85% of activity after storage in the 50 mM CHES buffer (pH 9.0) at room temperature for 2 months.
Efremenko
Elena N
EN
Chemical Enzymology Division of Chemical Department, M.V. Lomonosov Moscow State University, Moscow, 119899, Russia. efremenko@enzyme.chem.msu.ru
Lozinsky
Vladimir I
VI
Sergeeva
Viktoriya S
VS
Plieva
Fatima M
FM
Makhlis
Tatiana A
TA
Kazankov
Grigori M
GM
Gladilin
Alexander K
AK
Varfolomeyev
Sergei D
SD
D001671:Biochemistry / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D005782:Gels / Q000737:chemistry*; D006583:Hexadimethrine Bromide / Q000737:chemistry*; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry*; D011549:Pseudomonas / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D005782:Gels; D000431:Ethanol; D006583:Hexadimethrine Bromide; C045290:phosphorylphosphatase; D010744:Phosphoric Monoester Hydrolases