Genomic dissection reveals locus response to stress for mammalian acetylcholinesterase.
The mammalian acetylcholinesterase (ACHE) locus was investigated using computational predictive methods and experiments of reverse transcription polymerase chain reaction (RT-PCR). Computational analysis identified two genes downstream to ACHE, an inversely oriented arsenite resistance gene homologue (ARS), and a novel previously unidentified gene (PIX), co-oriented with ACHE. Experimental evidence shows coregulation of murine ACHE and ARS following confined swim, indicating coordinated locus response to stress, that is possibly mediated by altered cholinergic neurotransmission.
Grant
A D
AD
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel.
Shapira
M
M
Soreq
H
H
D000109:Acetylcholine / Q000096:biosynthesis*; D000110:Acetylcholinesterase / Q000235:genetics* / Q000378:metabolism; D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D053501:Arsenite Transporting ATPases; D001921:Brain / Q000201:enzymology*; D018797:Cell Cycle Proteins / Q000235:genetics; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D005796:Genes / Q000235:genetics; D016678:Genome; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics; D016623:Ion Pumps / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics; D009474:Neurons / Q000201:enzymology*; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D064067:Rho Guanine Nucleotide Exchange Factors; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013312:Stress, Physiological / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D009435:Synaptic Transmission / Q000235:genetics*
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D020662:Guanine Nucleotide Exchange Factors; D016623:Ion Pumps; D009097:Multienzyme Complexes; D020033:Protein Isoforms; D012333:RNA, Messenger; D064067:Rho Guanine Nucleotide Exchange Factors; D000110:Acetylcholinesterase; D053501:Arsenite Transporting ATPases; C506972:Asna1 protein, mouse; D000109:Acetylcholine