title: Shiga toxin genes in avian Escherichia coli.
abstract: The purpose of this study was to determine the presence of stx genes in avian pathogenic Escherichia coli (APEC). We examined 97 APEC isolates: 34 from lesions of avian cellulitis, 31 from avian septicemia, 13 from swollen head syndrome (SHS) in chickens, and 19 from diseased turkeys. We also examined five isolates from the feces of healthy chickens. All 102 E. coli isolates were tested for the presence of stx genes by PCR amplification and by colony blots using probes specific for stx1 and stx2. Fifty-three percent (52) of the 97 APEC carried stx gene sequences: one isolate carried stx2 sequences, two carried both stx1 and stx2 sequences, and the remaining 49 isolates carried only stx1 sequences. Twenty-six isolates were positive by both hybridization and PCR amplification, 10 were positive by PCR only, and 16 were positive by hybridization only. All the stx-positive isolates were negative by PCR for the eae and E-hlyA genes. The five isolates from healthy chickens were all negative for stx. All 13 SHS isolates were positive for the stx1 gene and had low titres for cytotoxicity in the Vero cell assay (VCA). Other stx-positive isolates were negative in the VCA. The stx1 gene from one SHS E. coli isolate was cloned and sequenced and shown to be identical to that of the stx gene of Shigella dysenteriae. The observations indicate that stx1 gene sequences are widespread among APEC but that cytotoxicity on Vero cells is uncommon.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Ont., Canada.
authors.forename: V R
authors.initials: VR
authors.lastname: Parreira
authors.forename: C L
authors.initials: CL
authors.lastname: Gyles
mesh_terms: D000818:Animals; D002645:Chickens; D002522:Chlorocebus aethiops; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D009500:Neutralization Tests / Q000662:veterinary; D009693:Nucleic Acid Hybridization; D019081:O Antigens; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D022622:Shiga Toxin 1 / Q000096:biosynthesis / Q000235:genetics*; D022641:Shiga Toxin 2 / Q000096:biosynthesis / Q000235:genetics*; D014422:Turkeys; D014709:Vero Cells
chemical_list: D004269:DNA, Bacterial; D019081:O Antigens; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2