Flt-3 ligand (FL) drives differentiation of rat bone marrow-derived dendritic cells expressing OX62 and/or CD161 (NKR-P1).
Bone marrow-derived dendritic cells (DC) of the rat have not been as well characterized as those from the mouse. Here, large quantities of bone marrow-derived rat DC were generated when Flt-3 ligand (FL) was used as an adjunct to granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). These cells displayed a typical DC phenotype, expressing MHC class II, CD54, CD80, CD86, and CD11b/c. These DC also uniformly expressed low levels of CD161 and expressed OX62 in a bimodal distribution. Few cells were recovered from cultures grown without FL, and they failed to express OX62 or CD161. The DC generated with FL were more potent antigen-presenting cells in mixed lymphocyte cultures than cells grown without FL, and among FL-derived cells, the OX62+ cells were slightly more stimulatory than OX62- cells. Thus, FL is a useful cytokine for obtaining large quantities of functional rat DC subsets in vitro.
Brissette-Storkus
Cynthia S
CS
Eye and Ear Institute and Department of Ophthalmology, Brain Tumor Center of the University of Pittsburgh Cancer Institute, Pennsylvania 15213, USA. cbstork@pitt.edu
Kettel
J C
JC
Whitham
T F
TF
Giezeman-Smits
K M
KM
Villa
L A
LA
Potter
D M
DM
Chambers
William H
WH
D000818:Animals; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000502:physiology*; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D016130:Immunophenotyping; D037102:Lectins / Q000276:immunology; D037181:Lectins, C-Type; D008024:Ligands; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008565:Membrane Proteins / Q000502:physiology*; D055651:NK Cell Lectin-Like Receptor Subfamily B; D051381:Rats; D011916:Rats, Inbred F344
D015703:Antigens, CD; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D037102:Lectins; D037181:Lectins, C-Type; D008024:Ligands; D008565:Membrane Proteins; D055651:NK Cell Lectin-Like Receptor Subfamily B; C459465:OX-62 antigen, rat; C084881:flt3 ligand protein; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; C042739:antigens, CD200