Expression of Human Single Chain Interleukin 12 Gene in Drosophila Cells.
The hscIL-12 gene(no signal sequence), amplified by PCR, was subcloned into an expressing vector pMT/Bip/V5. The recombinant plasmid was transferedinto Drosophila cell line S2. The secreted hscIL-12 fusion protein was identified by Western blot, and the bioactivity of hscIL-12 was investigated by lymphocyte proliferation assay. The results showed hscIL-12 expressed in S2 cells could stimulate lymphocyte proliferation in dose-dependent manner. hscIL-12 has bioactivity identical to natural interleukin 12. These findings suggest that hscIL-12 is promising in theoretical reseach and in clinical use.
Jin
Li
L
Central Laboratory of Shanghai No.1 Hospital, Shanghai 200080, China. jinli73@yahoo.com
Qi
Xu
X
Lai
Bao-Chang
BC
Si
Lü-Sheng
LS
Wang
Yi-Li
YL