Genotype of CYP3AP1 associated with CYP3A activity in Chinese Han population.
OBJECTIVE
To investigate the distribution of genotype of CYP3AP1 in Chinese Han population and the correlation with CYP3A activity.


METHODS
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed in CYP3AP1 genotype analysis; using midazolam as probe drug, CYP3A activity of 191 Chinese healthy subjects was measured by plasma 1'-hydroxymidazolam/midazolam (1'-OH-MDZ/MDZ) ratio at 1 h after oral administration of 7.5 mg midazolam.


RESULTS
There was significant difference of CYP3A activity in different genotypes of CYP3AP1 in vivo (P <0.05). The activity of CYP3A in homozygous A(-44) (CYP3AP1*3/CYP3AP1*3) is lower than heterozygous A(-44)G (CYP3AP1*1/CYP3AP1*3), and the CYP3A activity in homozygous G(-44) (CYP3AP1*1/CYP3AP1*1) is t he highest.


CONCLUSIONS
There was association between the genotype of CYP3AP1 and increased activity of CYP3A in vivo.
Zhu
Bing
B
Pharmacogenetics Research Institute, Xiang-Ya School of Medicine, Central South University, Changsha 410078, China.
Chen
Guo-Lin
GL
Chen
Xiao-Ping
XP
He
Nan
N
Liu
Zhao-Qian
ZQ
Jiang
Chang-Hong
CH
Wang
Dan
D
Zhou
Hong-Hao
HH
D000293:Adolescent; D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics* / Q000378:metabolism*; D044466:Asian People / Q000235:genetics; D051544:Cytochrome P-450 CYP3A; D005006:Ethnicity; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008874:Midazolam / Q000097:blood* / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D001189:Aryl Hydrocarbon Hydroxylases; C465138:Cyp3a23-3a1 protein, rat; D051544:Cytochrome P-450 CYP3A; D010089:Oxidoreductases, N-Demethylating; D008874:Midazolam