[The detection of genome polymorphism in Stachys species using RAPD].
Molecular genome analysis was for the first time carried out in the genus Stachys. RAPD analysis proved to be suitable for identifying the species-specific markers, studying the interspecific DNA polymorphism, and detecting the genetic changes that arise during in vitro culturing of Stachys sieboldii. In addition, RAPD was used for screening genetic variation in S. sieboldii regenerants obtained at various phytohormone concentrations. High cytokinin concentrations and multiple regeneration were shown to induce genetic changes detectable with RAPD patterns. High DNA polymorphism was demonstrated for two types of S. sieboldii callus cultures and for plants regenerated from a callus culture.
Kochieva
E Z
EZ
Vavilov Institute of General Genetics, Russian Academy of Sciences, Timiryazev Agricultural Academy, Moscow, Russia. kochieva@vigg.ru
Khusseĭn
I A
IA
Legkobit
M P
MP
Khadeeva
N V
NV
D018744:DNA, Plant / Q000302:isolation & purification; D014644:Genetic Variation; D018745:Genome, Plant; D006109:Poaceae / Q000235:genetics*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique
D018744:DNA, Plant
Ispol'zovanie RAPD analiza dlia vydeleniia genomnogo polimorfizma u predstaviteleĭ roda Stachys.