title: Inhibitory effects of lemon grass (Cymbopogon citratus, Stapf) extract on the early phase of hepatocarcinogenesis after initiation with diethylnitrosamine in male Fischer 344 rats.
abstract: Effects of lemon grass extract (LGE) on hepatocarcinogenesis were examined in male Fischer 344 rats, administered diethylnitrosamine (DEN) at three weekly intraperitoneal doses of 100 mg/kg body weight and partially hepatectomized at the end of week 5. LGE was given at dietary concentrations of 0, 0.2, 0.6 or 1.8% from the end of week 4 for 10 weeks. All rats were sacrificed at the end of week 14. LGE reduced the number of putatively preneoplastic, glutathione S-transferase placental form-positive lesions and the level of oxidative hepatocyte nuclear DNA injury, as assessed in terms of 8-hydroxydeoxyguanosine production. In contrast, LGE did not affect the size of the preneoplastic lesions, hepatocyte proliferative activity, activities of phase II enzymes or hepatocyte extra-nuclear oxidative injury. These results suggest inhibitory effects of LGE on the early phase hepatocarcinogenesis in rats after initiation with DEN.
authors.affiliation: Department of Oncological Pathology, Cancer Center, Nara Medical University, Kashihara, 634-8521, Nara, Japan.
authors.forename: Rawiwan
authors.initials: R
authors.lastname: Puatanachokchai
authors.forename: Hideki
authors.initials: H
authors.lastname: Kishida
authors.forename: Ayumi
authors.initials: A
authors.lastname: Denda
authors.forename: Nao
authors.initials: N
authors.lastname: Murata
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Konishi
authors.forename: Usanee
authors.initials: U
authors.lastname: Vinitketkumnuen
authors.forename: Dai
authors.initials: D
authors.lastname: Nakae
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D004052:Diethylnitrosamine; D005982:Glutathione Transferase / Q000032:analysis; D006498:Hepatectomy; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D019684:Magnoliopsida; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D016588:Anticarcinogenic Agents; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D004052:Diethylnitrosamine; D005982:Glutathione Transferase