Molecular Cloning of a Gene Suppressed by Heat Shock and Its Expression Characteristics.
On the base of a cDNA fragment cloned from a human osteosarcoma cell line (HOS-8603) which was taken as the model of heat shock by DDRT-PCR, the cDNA library was screened by the probe made from this fragment, and the colony containing full length cDNA of this cDNA fragment (HSSG-1) was identified. Sequencing data indicated that this cDNA consisted of 1 456 bp, coding for 276 amino acids. Computer aided homology analysis did not find the same sequence published. The result of RNA dot hybridization suggested that HSSG-1 was expressing in many kinds of organs and cells, and the down regulating of its expression level caused by heat shock was general. Therefore, this may be a gene that is suppressed by heat shock.
Dai
Jian-Xin
JX
Department of Molecular Genetics, Second Military Medical University, Shanghai 200433, China. daijx@sina.com
Wang
Yi-Lun
YL
Zhou
Feng-Juan
FJ
Lu
De-Ru
DR
Liu
Yu-Jian
YJ
Xu
Ren-Bao
RB