Cytotoxic, hypoglycemic activity and phytochemical analysis of Rubus imperialis (Rosaceae).
Rubus imperialis, Artemia salina, 3-O-methylellagic-4'-O-alpha-rhamnose Acid Screening of different extracts, fractions and compounds from Rubus imperialis Chum. Schl. (Rosaceae) has been conduced using the brine shrimp microwell cytotoxicity assay. Three parts of the plant (methanolic extract from leaves, roots and stems), three fractions from roots (hexane, ethyl acetate and butanol) and three isolated compounds (niga-ichigoside F1, 23-hydroxytormentic acid, ellagic acid derivative) were tested. The most promising material (LC50 <1000 microg/ml) were the methanolic extract and ethyl acetate fraction from roots. However, there was little correlation observed in the degree of toxicities observed between the isolated compounds. On the other hand, the cytotoxicity and in vivo assays confirmed the hypoglycemic activity of methanolic extract and validated the Brazilian popular use of R. imperialis as an antidiabetic agent.
Kanegusuku
Márcia
M
Departamento de Bioquímica, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Benassi
Jean Carlo
JC
Pedrosa
Rozangela Curi
RC
Yunes
Rosendo Augusto
RA
Filho
Valdir Cechinel
VC
Maia
Alcíbia Azevedo
AA
de Souza
Márcia Maria
MM
Delle Monache
Franco
F
Niero
Rivaldo
R
D000818:Animals; D001156:Artemia; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D001938:Brazil; D002470:Cell Survival / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D027824:Rosaceae / Q000737:chemistry*
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010936:Plant Extracts