[Typings of Campylobacter jejuni isolated from patients of 2 outbreak cases by genotypic and phenotypic methods].
We compared Campylobacter jejuni strains isolated from the patient stools associated with two food-borne diarrheal outbreak cases by the serotypic methods (Lior and Penner systems) and the genotypic methods (restriction fragment length polymorphism (RFLP) of flaA gene and pulsed-field gel electrophoresis (PFGE)). Fla-RFLP was based on the digestion of 410 bp DNA fragment by MboI restriction enzyme amplified from a 5' portion of C. jejuni flaA gene. Six distinctive fla-RFLP patterns were identified by examining 29 serotype reference strains and 58 strains isolated from the patients infected with C. jejuni independently. In the first outbreak case, 4 isolates were shown to be the same patterns each other by the fla-RFLP and PFGE, and by the Lior serotyping, except the Penner system that serotyped into 2 distinct types. On the other hand, in the second case, out of 10 isolates, 5 isolates were identical by the both genotypic and the both serotypic methods, and 4 isolates were not differentiated by the fla-RFLP and Penner system, but were separated into 4 types by PFGE in a little difference. The rest isolate was completely different from the other isolates by the all of methods used now. The findings suggest that the second case occurred by the infection of at least 3 different strains of C. jejuni.
Saito
Shioko
S
Akita Prefectural Institute of Public Health.
Yatsuyanagi
Jun
J
Sato
Hiroyasu
H
Shiraishi
Hiroyuki
H
Amano
Ken-ichi
K
D000293:Adolescent; D001483:Base Sequence; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping / Q000379:methods*