title: The ABC transporter SpTUR2 confers resistance to the antifungal diterpene sclareol.
abstract: PDR5-like proteins represent one group of the ABC superfamily of transporters. Members of this group are present in plants and, due to the function of PDR5-related proteins in fungi in the excretion of xenobiotics (including antifungal agents), it has been proposed that they might play a similar role in plants in the response to and detoxification of herbicides and fungicides. However, until now no functional data has been presented showing an altered plant response to any herbicide or fungicide as a result of manipulating the expression of a PDR5-like gene in plants. In this paper, we show that the plant SpTUR2 PDR5-like ABC transporter is localised to the plasma membrane and that expression of this protein in Arabidopsis leads to the acquisition of resistance to the diterpenoid antifungal agent sclareol. These data both define a possible endogenous substrate for this transporter and highlight the potential of manipulating plant chemical resistance via modulating the expression of specific PDR5-like transporters.
authors.affiliation: Department of Plant Biochemistry and Physiology, Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH) Zürich, Universitätsstrasse 2, Switzerland.
authors.forename: Sybille
authors.initials: S
authors.lastname: van den Brûle
authors.forename: Axel
authors.initials: A
authors.lastname: Müller
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Fleming
authors.forename: Cheryl C
authors.initials: CC
authors.lastname: Smart
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000382:microbiology; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D004351:Drug Resistance / Q000235:genetics; D005658:Fungi / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant; D007150:Immunohistochemistry; D019684:Magnoliopsida / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology; D008565:Membrane Proteins / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified
chemical_list: D018528:ATP-Binding Cassette Transporters; D004224:Diterpenes; D008565:Membrane Proteins; D010940:Plant Proteins; C100878:TUR2 protein, Spirodela polyrrhiza; C109395:sclareolide; C070760:sclareol