Impact of cytokines on replication in the thymus of primary human immunodeficiency virus type 1 isolates from infants.
Early infection of the thymus with the human immunodeficiency virus (HIV) may explain the more rapid disease progression among children infected in utero than in children infected intrapartum. Therefore, we analyzed infection of thymocytes in vitro by HIV type 1 primary isolates, obtained at or near birth, from 10 children with different disease outcomes. HIV isolates able to replicate in the thymus and impact thymopoiesis were present in all infants, regardless of the timing of viral transmission and the rate of disease progression. Isolates from newborns utilized CCR5, CXCR4, or both chemokine receptors to enter thymocytes. Viral expression was observed in discrete thymocyte subsets postinfection with HIV isolates using CXCR4 (X4) and isolates using CCR5 (R5), despite the wider distribution of CXCR4 in the thymus. In contrast to previous findings, the X4 primary isolates were not more cytopathic for thymocytes than were the R5 isolates. The cytokines interleukin-2 (IL-2), IL-4, and IL-7 increased HIV replication in the thymus by inducing differentiation and expansion of mature CD27(+) thymocytes expressing CXCR4 or CCR5. IL-2 and IL-4 together increased expression of CXCR4 and CCR5 in this population, whereas IL-4 and IL-7 increased CXCR4 but not CCR5 expression. IL-2 plus IL-4 increased the viral production of all pediatric isolates, but IL-4 and IL-7 had a significantly higher impact on the replication of X4 isolates compared to R5 isolates. Our studies suggest that coreceptor use by HIV primary isolates is important but is not the sole determinant of HIV pathogenesis in the thymus.
Pedroza-Martins
Livia
L
Department of Microbiology, Immunology, and Molecular Genetics, UCLA AIDS Institute, USA.
Boscardin
W John
WJ
Anisman-Posner
Deborah J
DJ
Schols
Dominique
D
Bryson
Yvonne J
YJ
Uittenbogaart
Christel H
CH
D002454:Cell Differentiation; D002478:Cells, Cultured; D015658:HIV Infections / Q000503:physiopathology / Q000821:virology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007378:Interleukins / Q000494:pharmacology*; D019713:Receptors, CCR5 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D013601:T-Lymphocytes / Q000502:physiology / Q000821:virology; D013950:Thymus Gland / Q000166:cytology / Q000821:virology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D007378:Interleukins; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7