RASPBERRY3 gene encodes a novel protein important for embryo development.
We identified a new gene that is interrupted by T-DNA in an Arabidopsis embryo mutant called raspberry3. raspberry3 has "raspberry-like" cellular protuberances with an enlarged suspensor characteristic of other raspberry embryo mutants, and is arrested morphologically at the globular stage of embryo development. The predicted RASPBERRY3 protein has domains found in proteins present in prokaryotes and algae chloroplasts. Computer prediction analysis suggests that the RASPBERRY3protein may be localized in the chloroplast. Complementation analysis supports the possibility that the RASPBERRY3 protein may be involved in chloroplast development. Our experiments demonstrate the important role of the chloroplast, directly or indirectly, in embryo morphogenesis and development.
Apuya
Nestor R
NR
Department of Plant and Microbial Biology, University of California-Berkeley, Berkeley, CA 94720, USA.
Yadegari
Ramin
R
Fischer
Robert L
RL
Harada
John J
JJ
Goldberg
Robert B
RB
Harada
John H
JH
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D001483:Base Sequence; D002736:Chloroplasts / Q000235:genetics; D002934:Cinnamates; D003001:Cloning, Molecular; D046508:Culture Techniques; D004269:DNA, Bacterial / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D005816:Genetic Complementation Test; D006921:Hygromycin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D007612:Kanamycin / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D002934:Cinnamates; D004269:DNA, Bacterial; D018076:DNA, Complementary; D018744:DNA, Plant; C464508:RSY3 protein, Arabidopsis; C036483:T-DNA; D006921:Hygromycin B; C026273:hygromycin A; D007612:Kanamycin