Testing protein permeability of dialysis membranes using SDS-PAGE.
BACKGROUND
Permeability of dialysis membranes for high molecular weight compounds should be similar to that of the glomerular membrane in order to remove uremic toxins like the human kidney does. In order to evaluate permeability of high-flux dialysis membranes SDS-PAGE is applied for examination of filtrate of dialysers during routine dialysis with different membranes.


METHODS
SDS-PAGE analysis is performed with silver staining method according to the modification of Melzer (5) and consecutive laser densitometry.


RESULTS
The protein pattern of filtrate from dialysis membranes is similar to that of the glomerular membrane containing IgG, transferrin, albumin, alpha-1-microglobulin, retinol binding protein and beta-2-microglobulin. Comparing different membranes there are considerable differences depending on cut-off, charge and adsorption capacity of the particular membrane. In all membranes tested permeability of proteins decreases during one treatment session.


CONCLUSIONS
Protein permeability of high-flux dialysis membranes is similar to the gloemerular membrane but modified according to pore-size, surface charge, adsorption and time on dialysis. In contrast to the glomerular membrane in each of the investigated membranes protein permeability decreases during function.
Mann
H
H
Institute of Experimental Nephrology, Aachen, Germany. helmut.mann@post.rwth-aachen.de
Melzer
H
H
Al-Bashir
A
A
Xu
X Q
XQ
Stiller
S
S
D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008567:Membranes, Artificial; D010539:Permeability; D011506:Proteins / Q000032:analysis*; D006435:Renal Dialysis / Q000295:instrumentation*; D012967:Sodium Dodecyl Sulfate; D001613:beta 2-Microglobulin / Q000032:analysis
D008567:Membranes, Artificial; D011506:Proteins; D001613:beta 2-Microglobulin; D012967:Sodium Dodecyl Sulfate