title: VCAM-1-positive microglia target oligodendrocytes at the border of multiple sclerosis lesions.
abstract: The distribution and lineage of vascular cell adhesion molecule-1 (VCAM-1)-positive cells was investigated in 43 lesions from the brain tissue of patients with multiple sclerosis (MS). Numerous VCAM-1-positive macrophages/microglia were detected at the edges of MS lesions. Quantitative analysis of 6 active, 7 chronic active, and 4 chronic inactive MS lesions identified most VCAM-1-positive cells at the actively demyelinating borders of active (102/mm3) and chronic active (29/mm3) lesions, but rarely in chronic inactive lesions (4/mm3). Further, approximately 17% of the VCAM-1-positive cells closely apposed or surrounded oligodendrocyte perikarya at the edges of active and chronic active lesions that were sites of ongoing demyelination. Endothelial cells were VCAM-1-negative in both lesion and non-lesion MS brain tissue. This report is the first to document direct microglial interaction with oligodendrocytes in MS.
authors.affiliation: Department of Neurosciences, Lerner Research Institute, The Cleveland Clinic Foundation, Ohio 44195, USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Peterson
authors.forename: Lars
authors.initials: L
authors.lastname: Bö
authors.forename: Sverre
authors.initials: S
authors.lastname: Mörk
authors.forename: Ansi
authors.initials: A
authors.lastname: Chang
authors.forename: Richard M
authors.initials: RM
authors.lastname: Ransohoff
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Trapp
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies; D002450:Cell Communication; D019070:Cell Lineage; D003711:Demyelinating Diseases / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D039041:Integrin alpha4beta1; D016023:Integrins / Q000378:metabolism; D008297:Male; D017628:Microglia / Q000737:chemistry* / Q000473:pathology*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000473:pathology*; D009836:Oligodendroglia / Q000473:pathology*; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D039041:Integrin alpha4beta1; D016023:Integrins; D016201:Receptors, Lymphocyte Homing; D019010:Vascular Cell Adhesion Molecule-1