title: Functional inactivation of the mouse nucleolar protein Bop1 inhibits multiple steps in pre-rRNA processing and blocks cell cycle progression.
abstract: Bop1 is a conserved nucleolar protein involved in rRNA processing and ribosome assembly in eukaryotes. Expression of its dominant-negative mutant Bop1 Delta in mouse cells blocks rRNA maturation and synthesis of large ribosomal subunits and induces a reversible, p53-dependent cell cycle arrest. In this study, we have conducted a deletion analysis of Bop1 and identified a new mutant, Bop1N2, that also acts as a potent inhibitor of cell cycle progression. Bop1N2 and Bop1 Delta are C-terminal and N-terminal deletion mutants, respectively, and share only 72 amino acid residues. Both mutant proteins are localized to the nucleolus and strongly inhibit rRNA processing, suggesting that activation of a cell cycle checkpoint by Bop1 mutants is linked to their inhibitory effects on rRNA and ribosome synthesis. By using these dominant-negative mutants as well as antisense oligonucleotides to interfere with endogenous Bop1, we identified specific rRNA processing steps that require Bop1 function in mammalian cells. Our data demonstrate that Bop1 is required for proper processing at four distinct sites located within the internal transcribed spacers ITS1 and ITS2 and the 3' external spacer. We propose a model in which Bop1 serves as an essential factor in ribosome formation that coordinates processing of the spacer regions in pre-rRNA.
authors.affiliation: Department of Molecular Genetics, University of Illinois College of Medicine, Chicago, Illinois 60607-7170, USA.
authors.forename: Zaklina
authors.initials: Z
authors.lastname: Strezoska
authors.forename: Dimitri G
authors.initials: DG
authors.lastname: Pestov
authors.forename: Lester F
authors.initials: LF
authors.lastname: Lau
mesh_terms: D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D002455:Cell Division; D015342:DNA Probes; D015536:Down-Regulation / Q000187:drug effects; D019084:Fluorescent Antibody Technique, Indirect; D051379:Mice; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000378:metabolism*; D017384:Sequence Deletion
chemical_list: D015342:DNA Probes; D009687:Nuclear Proteins; D016376:Oligonucleotides, Antisense; D012322:RNA Precursors; D012335:RNA, Ribosomal