title: Effect of heparin chain length on the interaction with tissue factor pathway inhibitor (TFPI).
abstract: Tissue factor pathway inhibitor (TFPI) is a heparin-binding protein involved in the extrinsic blood coagulation system. In order to elucidate the minimal size of heparin chain required for the interaction with TFPI, we prepared a series of heparin-derived oligosaccharides with tailored chain length ranged from disaccharide to eicosasaccharide after the successive treatments of heparin, including partial N-desulphation, deaminative cleavage with nitrous acid and gel-filtration. Affinity chromatography study of each oligosaccharide fraction using TFPI as the ligand indicated that increasing the degree of polymerisation causes increased affinity, and that a remarkable change in the affinity occurs between the decamers and dodecamers. Measurement of factor Xa inhibitory activity of TFPI in the presence of each oligosaccharide fraction indicated that the fractions shorter than dodecamers only slightly enhanced the TFPI activity for factor Xa inhibition, while the fractions larger than octadecamers had an effect comparable to full-length heparin. These were compatible to the results from the kinetic analyses of the interaction between TFPI and heparin-derived oligosaccharide with an evanescent wave-based biosensor system, IAsys, using a TFPI C-terminal peptide as the ligand.
authors.affiliation: Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Xu
authors.forename: Ryo
authors.initials: R
authors.lastname: Takano
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Nagai
authors.forename: Tadatoshi
authors.initials: T
authors.lastname: Yanagida
authors.forename: Kaeko
authors.initials: K
authors.lastname: Kamei
authors.forename: Hisao
authors.initials: H
authors.lastname: Kato
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kamikubo
authors.forename: Yo
authors.initials: Y
authors.lastname: Nakahara
authors.forename: Kosuke
authors.initials: K
authors.lastname: Kumeda
authors.forename: Saburo
authors.initials: S
authors.lastname: Hara
mesh_terms: D000595:Amino Acid Sequence; D015374:Biosensing Techniques; D002846:Chromatography, Affinity; D065427:Factor Xa Inhibitors; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism*; D008074:Lipoproteins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D065427:Factor Xa Inhibitors; D008074:Lipoproteins; D009844:Oligosaccharides; C051928:lipoprotein-associated coagulation inhibitor; D006493:Heparin