Chlorodinitrobenzene-mediated damage in the human erythrocyte membrane leads to haemolysis.
1-chloro-2,4-dinitrobenzene (CDNB), an intracellular glutathione-depleting agent, has been shown to have an adverse effect on erythrocyte membrane integrity. In the current study, we have demonstrated that CDNB caused haemolysis of human red blood cells (RBC) at higher concentrations (>or= 5 mM). The haemolysis induced by CDNB was preceded by the leakage of K(+) from the cells suggesting the colloid-osmotic nature of this lysis. The inclusion of molecules of increasing size in the extracellular media inhibited both the rate and extent of haemolysis thus supporting the proposal of CDNB-induced pore formation. The size of membrane lesions increased with an increase in the concentration of CDNB. SDS-PAGE demonstrated that CDNB causes the polymerisation and/or fragmentation of membrane proteins. Although CDNB has been shown to cause a drastic reduction in membrane thiols, our data suggest that the CDNB-induced formation of membrane disulfide bonds as a prima facie cause of permeability enhancement is unlikely.
Zou
Cheng-Gang
CG
School of Biological Sciences, University of New England, Armidale, NSW 2351, Australia.
Agar
Nihal S
NS
Jones
Graham Lloyd
GL
D004137:Dinitrochlorobenzene / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects*; D005978:Glutathione / Q000097:blood; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D009995:Osmosis; D009996:Osmotic Fragility; D011188:Potassium / Q000097:blood; D013438:Sulfhydryl Compounds / Q000097:blood; D013696:Temperature
D004137:Dinitrochlorobenzene; D013438:Sulfhydryl Compounds; D005978:Glutathione; D011188:Potassium