Cytotoxic effects of denture base materials on a permanent human oral epithelial cell line and on primary human oral fibroblasts in vitro.
OBJECTIVE
The objective of this study was to determine the cytocompatibility of three different extracts of denture base resins and to compare the cytotoxic effect of these materials on a human oral epithelial KB cell line and primary human oral fibroblasts derived from buccal mucosa.


METHODS
Set specimens from a heat-cured resin, a self-cured resin, and a light-cured resin were eluted with culture medium for 1, 3, and 5 days. Cytotoxicity was judged using tetrazolium bromide reduction assay.


RESULTS
The eluates from self-cured, heat-cured, and light-cured denture base resins were cytotoxic to primary human buccal fibroblast cultures and KB cells. Self-cured resin was the most toxic denture base material among the chemicals tested in all cultures. The cytotoxicity decreased in the order of self-cured resin > heat-cured resin > light-cured resin for KB cells. The rank for buccal fibroblast cells was self-cured resin > heat-cured resin > light-cured resin.


CONCLUSIONS
The influence of the cytotoxicity depended on the materials tested and the cell culture system used. The use of both permanent and primary cells is recommended for a better screening of the cytotoxic effects of denture base resins.
Huang
F M
FM
Department of Prosthodontics, Chung Shan Medical and Dental College Hospital, Taichung, Taiwan, Republic of China.
Tai
K W
KW
Hu
C C
CC
Chang
Y C
YC
D000180:Acrylic Resins / Q000633:toxicity*; D000704:Analysis of Variance; D002460:Cell Line; D002478:Cells, Cultured; D004396:Coloring Agents; D003470:Culture Media; D003764:Dental Materials / Q000633:toxicity*; D003819:Denture Bases; D004847:Epithelial Cells / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007624:KB Cells / Q000187:drug effects; D009061:Mouth Mucosa / Q000187:drug effects* / Q000473:pathology; D015203:Reproducibility of Results; D013223:Statistics as Topic; D013778:Tetrazolium Salts; D013844:Thiazoles
D000180:Acrylic Resins; D004396:Coloring Agents; D003470:Culture Media; D003764:Dental Materials; D013778:Tetrazolium Salts; D013844:Thiazoles; C060386:Triad resin; C040291:Lucitone; C022616:thiazolyl blue