Saccharomyces Rrm3p, a 5' to 3' DNA helicase that promotes replication fork progression through telomeric and subtelomeric DNA.
In wild-type Saccharomyces cerevisiae, replication forks slowed during their passage through telomeric C(1-3)A/TG(1-3) tracts. This slowing was greatly exacerbated in the absence of RRM3, shown here to encode a 5' to 3' DNA helicase. Rrm3p-dependent fork progression was seen at a modified Chromosome VII-L telomere, at the natural X-bearing Chromosome III-L telomere, and at Y'-bearing telomeres. Loss of Rrm3p also resulted in replication fork pausing at specific sites in subtelomeric DNA, such as at inactive replication origins, and at internal tracts of C(1-3)A/TG(1-3) DNA. The ATPase/helicase activity of Rrm3p was required for its role in telomeric and subtelomeric DNA replication. Because Rrm3p was telomere-associated in vivo, it likely has a direct role in telomere replication.
Ivessa
Andreas S
AS
Department of Molecular Biology, Princeton University, New Jersey 08544-1014, USA.
Zhou
Jin-Qiu
JQ
Schulz
Vince P
VP
Monson
Ellen K
EK
Zakian
Virginia A
VA
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000483:Alleles; D002384:Catalysis; D002453:Cell Cycle; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA / Q000096:biosynthesis; D004265:DNA Helicases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D004261:DNA Replication; D008957:Models, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000502:physiology*; D016615:Telomere / Q000502:physiology / Q000648:ultrastructure; D013997:Time Factors
D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; D000251:Adenosine Triphosphatases; C085463:PIF1 protein, S cerevisiae; C459081:Rrm3 protein, S cerevisiae; D004265:DNA Helicases