title: Dynamic docking and electron transfer between myoglobin and cytochrome b(5).
abstract: The interaction of trypsin-digested bovine cytochrome b(5) (cyt b(5)) with horse heart myoglobin (Mb) and the interprotein electron transfer (ET) between these redox partners have been studied to gain better understanding of ET processes between weakly bound protein partners. The bimolecular rate constant ( k(2)) for photo-induced ET between zinc-substituted Mb (ZnMb) and cyt b(5) decreases with increasing ionic strength, consistent with the predominantly electrostatic character of this complex. The formation of a protein-protein complex has been confirmed and the binding affinities of metMb and ZnMb for cyt b(5) have been measured by two techniques: (1)H NMR titrations at pH 6.0 give binding constants of K(a) approximately (1.0+/-0.1)x10(3) M(-1) for metMb and K(a) approximately (0.75+/-0.1)x10(3) M(-1) for ZnMb; isothermal calorimetry gives K(a) approximately (0.35+/-0.1)x10(3) M(-1) for ZnMb. Brownian dynamic (BD) simulations show that cyt b(5) binds over a broad surface of Mb that includes its heme edge. The experimental results are described in terms of a dynamic docking model which proposes that Mb binds cyt b(5) in a large ensemble of protein binding conformations, not one or a few dominant ones, but that only a small subset are ET reactive. Aided by the BD simulations, this model explains why k(2) decreases with increasing pH: increasing pH not only weakens the binding affinity but also reduces the number of binding conformations with high ET reactivity.
authors.affiliation: Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.
authors.forename: Zhao-Xun
authors.initials: ZX
authors.lastname: Liang
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Qing
authors.initials: Q
authors.lastname: Ning
authors.forename: Brian M
authors.initials: BM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D002417:Cattle; D015786:Cytochromes b5 / Q000737:chemistry* / Q000378:metabolism; D004579:Electron Transport; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008665:Metalloporphyrins / Q000737:chemistry; D008958:Models, Molecular; D009211:Myoglobin / Q000737:chemistry* / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D011487:Protein Conformation; D015032:Zinc / Q000737:chemistry / Q000378:metabolism
chemical_list: D005290:Ferric Compounds; D008665:Metalloporphyrins; D009211:Myoglobin; D015786:Cytochromes b5; D015032:Zinc