Using Kl LAC4 as a Reporter Gene in Candida albicans.
The lacZ gene which codes for beta-galactosidase from E.coli does not work in Candida albicans. In this work a reporter system with Kl LAC4 gene was contructed, coding for beta-galactosidase from Kluyveromyces lactis. Kl LAC4 gene was fused to the promoter of ADH1 of Candida albicans with the terminator sequence of ADH1 at the tail. The constructed plasmid, named pYPB1-LAC4, was used to transform Candida albicans. beta-galactosidase activity was measured by plate assay, when cultured on solid medium, and by photometric assay, when cultured in liquid medium. The results suggest that LAC4 of Kluyveromyces lactis can serve as a reporter gene in Candida albicans.
Chen
Xi
X
State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. jychen@sunm.shcnc.ac.cn
Chen
Jiang-Ye
JY