Some human B and T cell epitopes of bovine serum albumin, the major beef allergen.
Bovine serum albumin (BSA) is the major beef allergen. Since IgE and T cell recognitions are central to the specific immune response to allergens, the identification and immunologic characterization of B and T cell epitopes of BSA represent important steps in the development of treatments for beef allergy. Prior to our experiments, we hypothesized that BSA-specific antibodies and T cells react primarily with sequential epitopes in which the amino acid sequences differ greatly between bovine and human albumin. To clarify this hypothesis, 16 peptides corresponding to such regions were synthesized as candidate epitopes. Among them, at least two regions, aa336-345 and aa451-459, were found to be B cell (IgE-binding) epitopes. In inhibition ELISA experiments, EYAV (aa338-341) and LILNR (aa453-457) bound to patient IgE antibodies and were found to be the cores of the IgE-binding epitopes. Three regions, DDSPDLPKLKPDPNTLC (aa107-123), PHACYTSVFDKLKHLVDEP (aa364-382), and LSLILNRLC (aa451-459), were found to induce T cell proliferation in more than half of the patients tested. Of interest was that these three regions were also recognized by B cells. Information concerning human B and T cells epitopes can contribute greatly to the elucidation of the etiology of beef allergy.
Tanabe
Soichi
S
Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan. stanabe@hiroshima-u.ac.jp
Kobayashi
Yoko
Y
Takahata
Yoshihisa
Y
Morimatsu
Fumiki
F
Shibata
Rumiko
R
Nishimura
Toshihide
T
D000485:Allergens / Q000737:chemistry; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002417:Cattle; D002455:Cell Division; D002675:Child, Preschool; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D005260:Female; D005512:Food Hypersensitivity / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000737:chemistry; D007223:Infant; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013601:T-Lymphocytes / Q000378:metabolism*; D013997:Time Factors
D000485:Allergens; D000939:Epitopes; D010455:Peptides; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E