[Dependence of activities of polysaccharide hydrolases and oxidases from Cerrena unicolor on the source of carbon and aromatic acids in culture media].
The activities of carboxymethylcellulase and xylanase in the higher basidial fungus Cerrena unicolor grown in avicel-containing medium reached 1.95 and 1.50 units per mg protein, respectively, whereas in mannitol-containing medium they ranged from 0.02 to 0.05 units per mg protein. The activity of fungal beta-glucosidase depended on the carbon source in the culture medium and ranged from 2.1 units per mg protein in the presence of mannitol to 17.3 units per mg protein in the presence of avicel. In contrast to polysaccharides, easily metabolizable substrates (cellobiose, mannitol, and glucose) provided the highest rates of secretion of laccase (52.7-123.5 ncat per mg protein) and ligninase (22-106 units per mg protein). The addition of tangerine pomace, a substrate enriched with aromatic compounds, to the culture medium caused an increase in the rate of bio-synthesis of laccase and ligninase to 862 ncat per ml and 557 units per ml, respectively. Aromatic compounds such as p-xylidine and veratric aldehyde increased the laccase activity of C. unicolor IBB 62 from 7.9 to 23.6 and 18.3 ncat per mg protein, respectively. Veratryl alcohol caused a sevenfold increase in the activity of Mn-dependent peroxidase in the culture medium.
Elisashvili
V
V
Durmishidze Institute of Biochemistry and Biotechnology, National Academy of Sciences of Georgia, Tbilisi, 380059 Georgia.
Kachlishvili
E
E
Bakradze
M
M
D024322:Amino Acids, Aromatic; D001487:Basidiomycota / Q000254:growth & development / Q000378:metabolism*; D001547:Benzaldehydes; D001592:Benzyl Alcohols; D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase; D002482:Cellulose; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism*; D042845:Laccase; D008353:Mannitol; D010088:Oxidoreductases / Q000096:biosynthesis / Q000378:metabolism*; D010105:Oxygenases / Q000096:biosynthesis; D010544:Peroxidases / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D013379:Substrate Specificity; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000378:metabolism
D024322:Amino Acids, Aromatic; D001547:Benzaldehydes; D001592:Benzyl Alcohols; D003470:Culture Media; D011134:Polysaccharides; D002475:Cellobiose; D008353:Mannitol; D002482:Cellulose; D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase; D010105:Oxygenases; C044391:ligninase; D006867:Hydrolases; D006026:Glycoside Hydrolases; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase; C019826:carboxymethylcellulase; D005947:Glucose; C042197:veratryl alcohol; C427725:veratraldehyde
Zavisimost' aktivnosti polisakharidgidrolaz i oksidaz Cerrena unicolor ot istochnika ugleroda i aromaticheskikh soedineniĭ v srede.