title: Early cytokine response of gnotobiotic piglets to Salmonella enterica serotype typhimurium.
abstract: Cytokine response against Salmonella Typhimurium is traditionally studied in conventional animals. Germ-free animals, however, enable to study response against infection without background effect of other microorganisms. Plasma and ileal inflammatory cytokines in germ-free piglets orally infected with virulent LT2 strain or, with a non-virulent SF1591 rough mutant were quantified by ELISA. In plasma and ileal washes, IFN-gamma levels significantly increased in both infected groups. TNF-alpha and IL-18 were mostly missing in plasma 24 h after infection. In the ileum, IFN-gamma, TNF-alpha, and IL-1beta were induced mainly by the virulent strain, whereas IL-18 was induced in highest quantity by non-virulent Salmonella. These data confirmed an important role of IFN-gamma, as well as other inflammatory cytokines in early stage of salmonellosis.
authors.affiliation: Department of Immunology and Gnotobiology, Institute of Microbiology, Nový Hrádek, Czech Republic. splichal@biomed.cas.cz
authors.forename: Igor
authors.initials: I
authors.lastname: Splíchal
authors.forename: Ilja
authors.initials: I
authors.lastname: Trebichavský
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Muneta
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Mori
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005856:Germ-Free Life / Q000276:immunology*; D007082:Ileum / Q000276:immunology / Q000382:microbiology; D007371:Interferon-gamma / Q000097:blood; D012481:Salmonella Infections, Animal / Q000097:blood / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology*; D013552:Swine; D013556:Swine, Miniature; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma