Detection of aminotransferase activity of Propionibacterium freudenreichii after SDS-PAGE.
Aminotransferases (ATs) had previously been detected after native electrophoresis. We show now that aminotransferase(s) of Propionibacterium freudenreichii can be detected after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Moreover, it retained a high activity (84%) in the presence of 0.23% SDS, contrary to what was observed for aminotransferase(s) of Bifidobacterium bifidum (54%) and of six other cheese-related species (0-20%).
Thierry
Anne
A
Laboratoire de Recherches de Technologie Laitière, I.N.R.A., 65 rue de Saint-Brieuc, 35042 Rennes Cedex, France. athierry@rennes.inra.fr
Maillard
Marie-Bernadette
MB
Lortal
Sylvie
S
D001644:Bifidobacterium / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D051276:Leucine Transaminase; D008970:Molecular Weight; D011424:Propionibacterium / Q000201:enzymology*; D000637:Transaminases / Q000032:analysis* / Q000378:metabolism
D000637:Transaminases; D051276:Leucine Transaminase