Towards quantitative assays with peptide chips: a surface engineering approach.
The development of peptide and protein microarrays has created enormous opportunities in biomedical research. Current chip-based assays are well suited for identifying candidate protein or enzyme activities but still require conventional solution phase experiments to validate hits. Here, three surface-engineering strategies for microarray design are described and are illustrated in the development of a peptide chip for the quantitative analysis of kinase activity on solid support. These strategies promise to widen the application of microarrays by permitting the evaluation of hits in a chip-based format.
Houseman
Benjamin T
BT
Dept of Chemistry and the Institute for Biophysical Dynamics, The University of Chicago, IL 60637, USA.
Mrksich
Milan
M
D000595:Amino Acid Sequence; D004800:Enzymes, Immobilized / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods / Q000639:trends; D011505:Protein-Tyrosine Kinases / Q000032:analysis / Q000737:chemistry*; D013379:Substrate Specificity; D013499:Surface Properties
D004800:Enzymes, Immobilized; D010455:Peptides; D011505:Protein-Tyrosine Kinases