Evidence that the putative alpha-glucosidase of Thermotoga maritima MSB8 is a pNP alpha-D-glucuronopyranoside hydrolyzing alpha-glucuronidase.
The gene (agu) encoding p-nitrophenyl alpha-D-glucuronopyranoside (pNP-GUA) hydrolyzing alpha-glucuronidase of the hyperthermophilic bacterium Thermotoga maritima was cloned and expressed in Escherichia coli. The recombinant enzyme was purified and characterized. The gene previously designated as putative alpha-glucosidase was found to code for a protein that had no alpha-glucosidase activity. It showed a rare activity profile with its ability to hydrolyze pNP-GUA, an activity not known in the alpha-glucuronidases from microbial sources. This is the first report on the occurrence of an alpha-glucuronidase which belongs to the family 4 of glycosyl hydrolases.
Suresh
Cuddapah
C
Enzyme Laboratory, National Food Research Institute, Ibaraki, Tsukuba, Japan.
Rus'd
Ahmed Abu
AA
Kitaoka
Motomitsu
M
Hayashi
Kiyoshi
K
D004229:Dithiothreitol / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006026:Glycoside Hydrolases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D008345:Manganese / Q000378:metabolism; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D020124:Thermotoga maritima / Q000201:enzymology*
D007544:Isopropyl Thiogalactoside; D008345:Manganese; D006026:Glycoside Hydrolases; C096734:alpha-glucuronidase; D004229:Dithiothreitol