In vivo and in organello assessment of OXPHOS activities.
Mitochondrial OXPHOS defects are responsible for a large group of human diseases and have been associated with degenerative disorders and aging. The accurate in vivo and in organello biochemical assessment of the OXPHOS system is necessary for the diagnosis and investigation of such conditions. Here I describe a set of accurate polarographic and spectrophotometric assays that use relatively small amounts of biological material (cells or isolated mitochondria) and discuss the biochemical parameters appropriate for discriminating partial OXPHOS defects.
Barrientos
Antoni
A
Department of Biological Sciences, Columbia University, New York, NY 10027, USA. ab775@columbia.edu
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004579:Electron Transport; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology*; D008954:Models, Biological; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D011048:Polarography / Q000379:methods*; D013053:Spectrophotometry / Q000379:methods*