title: Addition of Polybrene improves stability of organophosphate hydrolase immobilized in poly(vinyl alcohol) cryogel carrier.
abstract: Organophosphate hydrolase, covalently attached to the beads of poly(vinyl alcohol) cryogel in the presence of Polybrene, was fivefold more stable in 15% (v/v) ethanol solution than the free enzyme. Immobilized biocatalyst, prepared with an addition of Polybrene, retained a half of its initial activity in 50% (v/v) aqueous ethanol solution, 90% of activity during 10 working cycles of Paraoxon hydrolysis and 85% of activity after storage in the 50 mM CHES buffer (pH 9.0) at room temperature for 2 months.
authors.affiliation: Chemical Enzymology Division of Chemical Department, M.V. Lomonosov Moscow State University, Moscow, 119899, Russia. efremenko@enzyme.chem.msu.ru
authors.forename: Elena N
authors.initials: EN
authors.lastname: Efremenko
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Lozinsky
authors.forename: Viktoriya S
authors.initials: VS
authors.lastname: Sergeeva
authors.forename: Fatima M
authors.initials: FM
authors.lastname: Plieva
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Makhlis
authors.forename: Grigori M
authors.initials: GM
authors.lastname: Kazankov
authors.forename: Alexander K
authors.initials: AK
authors.lastname: Gladilin
authors.forename: Sergei D
authors.initials: SD
authors.lastname: Varfolomeyev
mesh_terms: D001671:Biochemistry / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D005782:Gels / Q000737:chemistry*; D006583:Hexadimethrine Bromide / Q000737:chemistry*; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry*; D011549:Pseudomonas / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D005782:Gels; D000431:Ethanol; D006583:Hexadimethrine Bromide; C045290:phosphorylphosphatase; D010744:Phosphoric Monoester Hydrolases