An improved DNA-based test for detection of the codon 616 mutation in the alpha cyclic GMP phosphodiesterase gene that causes progressive retinal atrophy in the Cardigan Welsh Corgi.
The aim of the study was to develop an improved test to detect the codon 616 gene mutation in the alpha cyclic GMP phosphodiesterase gene that causes progressive retinal atrophy in the Cardigan Welsh Corgi. We studied 10 control dogs of known genotype at codon 616 of the alpha cyclic GMP phosphodiesterase gene and 80 Cardigan Welsh Corgis of unknown genotype. A polymerase chain reaction (PCR) utilizing a mismatched primer was designed so that it introduced a HinfI restriction enzyme digestion site into the PCR product only if the normal gene sequence was present, the restriction site was not introduced if the codon 616 mutation was present. An additional HinfI site present in the amplified section from both normal and mutant alleles acted as a positive control for restriction enzyme digestion. The PCR reliably amplified a portion of the alpha cyclic GMP phosphodiesterase gene spanning the codon 616 mutation site. Restriction enzyme digestion with HinfI and analysis on a suitable agarose gel reliably ascertained the genotype of the control dogs and was used to identify the genotype of a further 80 test dogs. An improved DNA-based test for detection of the codon 616 mutation in the alpha cyclic GMP phosphodiesterase gene that causes progressive retinal atrophy in the Cardigan Welsh Corgi has been designed. This overcomes potential problems that could be associated with allele-specific PCR tests such as that used previously in a diagnostic test for this gene mutation.
Petersen-Jones
Simon M
SM
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, 48824, USA. peter315@cvm.msu.edu
Entz
David D
DD
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000235:genetics; D000818:Animals; D001284:Atrophy / Q000235:genetics / Q000662:veterinary; D001947:Breeding; D003062:Codon; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D017931:DNA Primers; D004283:Dog Diseases / Q000175:diagnosis / Q000235:genetics*; D004285:Dogs; D005820:Genetic Testing / Q000379:methods / Q000662:veterinary; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012162:Retinal Degeneration / Q000235:genetics / Q000662:veterinary*; D017422:Sequence Analysis, DNA / Q000662:veterinary
D003062:Codon; D017931:DNA Primers; D004247:DNA; D015106:3',5'-Cyclic-GMP Phosphodiesterases