Epstein-Barr virus and lymphoid hematological disorders.
BACKGROUND
To study the role of Epstein-Barr virus (EBV) in the carcinogenesis of various lymphoid neoplasms in Taiwan, a nonisotopic in situ hybridization (ISH) technique was used to detect the expression of EBV-encoded small RNAs (EBERs) in various lymphoid neoplasms.


METHODS
We obtained the paraffin-embedded tissues of various hematological malignancies from the Department of Pathology, Tri-Service General Hospital. Nonisotopic ISH technique was employed to detect EBERs and immunohistochemical staining was performed to confirm the cell lineage.


RESULTS
Our data showed the frequency of EBV infection on various lymphoid malignancies as followed: Burkitt's lymphoma (BL) (10/18); non-Burkitt's Non-Hodgkin's lymphoma (3/42); non-Hodgkin's lymphoma, T cell type, nasal (NHLTN) (5/5); Hodgkin's disease (2/9); acute lymphocytic leukemia (1/24); chronic lymphocytic leukemia (1/15); and multiple myeloma (2/18). In the control group, none of the specimens from patients of acute myelocytic leukemia and myelodysplastic syndrome was positive for EBERs.


CONCLUSIONS
Among the various lymphoid neoplasms we studied, only BL (p < 0.05) and NHLTN (p < 0.05) were found to have an association with EBV statistically.
Hsieh
An-Tie
AT
Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Ho
Ching-Liang
CL
Chen
Yeu-Chin
YC
Kao
Wei-Yau
WY
Chao
Tsu-Yi
TY
D020031:Epstein-Barr Virus Infections / Q000150:complications; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D007938:Leukemia / Q000209:etiology / Q000821:virology*; D008223:Lymphoma / Q000209:etiology / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis
D012367:RNA, Viral