In silico determination of potential antisense targets for human beta-globin variants.
The functional characterization of available genomic sequences is the major task of the research in the post-genome era. This complex task requires an integrative approach of high-throughput systems with in vitro and in vivo models in order to have a reliable evaluation of the biological function. The oligonucleotide antisense technology is one of the most promising approaches for the investigation of gene function; the crucial point of antisense experiments is the identification of optimal target sites for hybridisation. In this paper we have applied a bioinformatic tool for the recognition of optimal antisense targets. In order to evaluate the effect of mutational events on target selection we have tested the program on a sample of human beta-hemoglobin variants. The proposed algorithm software will be integrated in a web based tool at the site: http://www.nettab.org/agewa.
Arrigo
Patrizio
P
CNR-Istituto Circuiti Elettronici, Genova, Italy. arrigo@ice.ge.cnr.it
Ivaldi
Giovanni
G
Cardo
Pasquale Paolo
PP
D000465:Algorithms; D005914:Globins / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics* / Q000378:metabolism; D012984:Software
D016376:Oligonucleotides, Antisense; D020033:Protein Isoforms; D005914:Globins