Magnetic resonance imaging of neuronal connections in the macaque monkey.
Recently, an MRI-detectable, neuronal tract-tracing method in living animals was introduced that exploits the anterograde transport of manganese (Mn2+). We present the results of experiments simultaneously tracing manganese chloride and wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) to evaluate the specificity of the former by tracing the neuronal connections of the basal ganglia of the monkey. Mn2+ and WGA-HRP yielded remarkably similar and highly specific projection patterns. By showing the sequential transport of Mn2+ from striatum to pallidum-substantia nigra and then to thalamus, we demonstrated MRI visualization of transport across at least one synapse in the CNS of the primate. Transsynaptic tract tracing in living primates will allow chronic studies of development and plasticity and provide valuable anatomical information for fMRI and electrophysiological experiments in primates.
Saleem
Kadharbatcha S
KS
Riken Brain Science Institute, 2-1 Hirosawa, Wakoshi, Saitama 351-0198, Japan. saleemk@pcg.wustl.edu
Pauls
Jon M
JM
Augath
Mark
M
Trinath
Torsten
T
Prause
Burkhard A
BA
Hashikawa
Tsutomu
T
Logothetis
Nikos K
NK
D000818:Animals; D001370:Axonal Transport / Q000187:drug effects / Q000502:physiology; D001479:Basal Ganglia / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D001931:Brain Mapping / Q000295:instrumentation / Q000379:methods*; D002712:Chlorides / Q000009:adverse effects; D005917:Globus Pallidus / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008253:Macaca mulatta / Q000033:anatomy & histology* / Q000502:physiology; D008279:Magnetic Resonance Imaging; D017895:Manganese Compounds / Q000009:adverse effects; D017072:Neostriatum / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D009434:Neural Pathways / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009498:Neurotoxins / Q000009:adverse effects; D017397:Prefrontal Cortex / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013378:Substantia Nigra / Q000166:cytology / Q000502:physiology; D013788:Thalamus / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013997:Time Factors; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
D002712:Chlorides; D017895:Manganese Compounds; D009498:Neurotoxins; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; C025340:manganese chloride