Native transfer RNA catalyzes Diels-Alder reaction.
In this paper we show that transfer ribonucleic acids (tRNAs) catalyze the Diels-Alder cycloaddition reaction. A new DNA oxidative damage product, 6-furfuryladenine (kinetin) or its riboside (diene), was transformed with dimethyl acetylenedicarboxylate or maleic anhydride (dienophile). The reaction proceeds in the presence of tRNA at high pressure but not at ambient condition. If so tRNA in prebiotic conditions (RNA world) had at least two functions: catalytic and a carrier of genetic information. It means that tRNA at high pressure shows catalytic properties and is a true Diels-Alderase.
Mielcarek
Michal
M
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12, 61704 Poznan, Poland.
Barciszewska
Miroslawa Z
MZ
Sałanski
Piotr
P
Stobiecki
Maciej
M
Jurczak
Janusz
J
Barciszewski
Jan
J
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D005075:Biological Evolution; D002384:Catalysis; D004249:DNA Damage; D004591:Electrophoresis, Polyacrylamide Gel; D007701:Kinetin; D008299:Maleic Anhydrides; D008956:Models, Chemical; D018384:Oxidative Stress; D011312:Pressure; D012343:RNA, Transfer / Q000378:metabolism*; D015003:Yeasts
D008299:Maleic Anhydrides; D012343:RNA, Transfer; D000225:Adenine; D007701:Kinetin