title: A naturally occurring alternative product of the mastermind locus that represses notch signalling.
abstract: The mastermind locus encodes a nuclear protein required in the Notch signalling pathway. In a screen for genes affecting wing pattern, we identified an EP element that directs expression of an alternatively spliced form of the mastermind transcript that we call mam[DN]. Unlike the conventional mam transcript, mam[DN] is spatially regulated in the developing embryonic nervous system and eye imaginal disc. mam[DN] corresponds to an endogenous transcript and encodes an alternate form of the Mam protein that dominantly interferes with activity of the conventional Mam protein. Mam[DN] blocks Notch signalling downstream from the activated form of Notch but cannot interfere with an activated form of Su(H), suggesting that Mam[DN] may interfere with the activity of a ternary complex involving Mam, Notch and Su(H).
authors.affiliation: European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Giráldez
authors.forename: Lidia
authors.initials: L
authors.lastname: Pérez
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Cohen
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D005123:Eye / Q000196:embryology / Q000378:metabolism; D017344:Genes, Insect; D008565:Membrane Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051880:Receptors, Notch; D012097:Repressor Proteins / Q000378:metabolism; D015398:Signal Transduction; D051155:Wnt1 Protein
chemical_list: D029721:Drosophila Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D051880:Receptors, Notch; D012097:Repressor Proteins; C088731:Su(H) protein, Drosophila; D051155:Wnt1 Protein; C085316:mam protein, Drosophila; C074622:wg protein, Drosophila