Identification of a KRAB-containing zinc finger protein, ZNF304, by AU-motif-directed display method and initial characterization in lymphocyte activation.
A novel human Krüppel-associated box (KRAB) type zinc finger protein encoding gene, ZNF304, was obtained by AU-motif-directed display and RACE. This gene, which contains a tandem AU motif in the 3' untranslated region, has an ORF 1977-bp long that codes for a putative 659 residue protein with an amino-terminal KRAB domain and 13 carboxyl-terminal C2H2 zinc finger units. The gene maps to chromosome 19q13.4, a region that contains the largest zinc finger cluster so far identified in the human genome. Structurally, ZNF304 is related to a family of repressor transcription factors. ZNF304 expression was higher in lymphoid tissues but it was also detected in the following tissues, ordered by abundance: thyroid, adrenal gland, prostate, pancreas, and skeletal muscle. Jurkat, U937, and THP1 cell lines showed a relatively low expression of ZNF304. By contrast, PBLs stimulated with PHA or PMA + ionomycin showed a biphasic expression with a sharp increase at 6 h. This induction was closely parallel to IFN-gamma expression and partially to IL-4 and IL-10. The tissue distribution and kinetics of induction suggest that ZNF304 may be involved in the regulation of lymphocyte activation.
Sabater
Lidia
L
Immunology Unit, Germans Trias i Pujol University Hospital, Fundació per a la Recerca Biomèdica Germans Trias i Pujol, Crtra. del Canyet s/n, 08916 Badalona, Spain.
Ashhab
Yaqoub
Y
Caro
Pepi
P
Kolkowski
Edgardo C
EC
Pujol-Borrell
Ricardo
R
Domínguez
Orlando
O
D000227:Adenine Nucleotides / Q000737:chemistry; D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods; D006801:Humans; D007223:Infant; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000096:biosynthesis; D014018:Tissue Distribution; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D014500:Uracil Nucleotides / Q000737:chemistry; D016335:Zinc Fingers
D000227:Adenine Nucleotides; D012333:RNA, Messenger; D014157:Transcription Factors; D014500:Uracil Nucleotides; C457777:ZNF304 protein, human