PDGF-BB enhances expression of, and reduces adhesion to, laminin-5 in vascular smooth muscle cells.
The laminin family of extracellular matrix (ECM) proteins plays crucial roles in regulating cellular growth, migration, and differentiation. We report here that laminin-5 is expressed in the tunica media of the rat aorta and pulmonary arteries. Using indirect immunofluorescence microscopy, Western blots, and RT-PCR analysis, we found that primary cultures of rat arterial smooth muscle cells express laminin-5 and deposit it into their insoluble ECM. These cells also attach strongly to laminin-5 via beta1 integrin receptors in 30 min adhesion assays. Laminin-5 expression in these cells is upregulated by growth factors in vitro and platelet-derived growth factor (PDGF-BB) stimulation reduces adhesion to laminin-5. As laminin-5 promotes enhanced migration of other cell types, the production of and adhesion to laminin-5 by vascular smooth muscle cells may play a role in the pathological growth and migration of these cells associated with restenosis following vascular injury.
Kingsley
K
K
Department of Biological Sciences, University of Nevada, Las Vegas, NV, USA.
Rust
W L
WL
Huff
J L
JL
Smith
R C
RC
Plopper
G E
GE
D000818:Animals; D001011:Aorta / Q000166:cytology; D000077214:Becaplermin; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D002478:Cells, Cultured; D004357:Drug Synergism; D016326:Extracellular Matrix Proteins / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation; D000097794:Kalinin
D000077214:Becaplermin; D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins; D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D000097794:Kalinin