Molecular diversity of the HLA-C gene identified in a caucasian population.
A DNA typing procedure, based on a two stage polymerase chain reaction-sequence-specific oligonucleotide probe (PCR-SSOP) typing strategy, has been developed and applied to DNA from 1000 healthy individuals from the Northern Ireland region. The two-stage procedure involves human leukocyte antigen (HLA-C) identification through the use of a medium resolution PCR-SSOP system, followed by four secondary group specific PCR-SSOP systems, to enable allele resolution. The PCR-SSOP systems were designed for the identification of HLA-Cw alleles with possible discrimination within exons 2 and 3 of the HLA-C gene, i.e., HLA-Cw*01-Cw*16. PCR-SSP tests were designed for the resolution of HLA-Cw*17 and -Cw*18 alleles. The systems can also be used independently of each other if selective allele resolution is required. HLA-Cw allele frequencies occurring within the Northern Ireland population have been compiled, along with estimations of HLA-B/Cw haplotype frequencies.
Williams
Fionnuala
F
Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Northern, Belfast, Ireland. fionnuala.williams@bll.n-i.nhs.uk
Meenagh
Ashley
A
Patterson
Chris
C
Middleton
Derek
D
D014644:Genetic Variation; D015236:HLA-C Antigens / Q000145:classification / Q000235:genetics*; D006801:Humans; D044465:White People / Q000235:genetics*
D015236:HLA-C Antigens