title: Penicillin-binding proteins 1a and 1b form independent dimers in Escherichia coli.
abstract: We report here that PBP1a can dimerize but does not interact with PBP1b to form PBP1a/PBP1b heterodimers in Escherichia coli. These findings support the idea of a relevant involvement of dimerization of both PBP1a and PBP1b during murein synthesis and suggest the existence of different peptidoglycan synthesis complexes.
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, UMR 5089 du CNRS. Institut National des Sciences Appliquées de Toulouse, Toulouse, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Charpentier
authors.forename: Christian
authors.initials: C
authors.lastname: Chalut
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Rémy
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Masson
mesh_terms: D001426:Bacterial Proteins; D002352:Carrier Proteins; D003001:Cloning, Molecular; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006602:Hexosyltransferases / Q000235:genetics / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D002267:Muramoylpentapeptide Carboxypeptidase; D046915:Penicillin-Binding Proteins; D046928:Peptidoglycan Glycosyltransferase; D010458:Peptidyl Transferases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D011994:Recombinant Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D046928:Peptidoglycan Glycosyltransferase; C478681:penicillin-binding protein 1B, E coli; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase; D002267:Muramoylpentapeptide Carboxypeptidase