Telomerase activity and its correlation with the proliferative potential of bone marrow in aplastic anemia in children.
OBJECTIVE
To explore telomerase activity and its association with the proliferative potential of hematopoietic stem cells in bone marrow (BM) in patients with aplastic anemia (AA).


METHODS
Telomerase activity of mononuclear cells separated from BM was determined with the TRAPeze kit. BM specimens from 22 cases with AA and 7 normal controls were included. SPSS10.0 was applied to analyze data derived from telomerase activity and colony-forming unit-granulocyte and monocyte.


RESULTS
The median telomerase activity level of BM in AA was 2-fold higher than that in normal controls. There was an inverse correlation in AA between telomerase activity and colony-forming unit (r = 0.78, p < 0.05). BM of chronic AA expressed higher telomerase than that of acute AA.


CONCLUSIONS
Telomerase activity in AA was increased and may be the result of the negative feedback of hematopoietic potential. Telomerase activity varied between the subtypes of AA. Telomerase activity was conversely correlated with the proliferative potential of BM in AA.
Shen
Jian Bin
JB
Department of Hematology, Xinhua Hospital, Shanghai Second Medical University, Shanghai, China. jianbinshen@etang.com
Tang
Jing Yang
JY
Zhao
Jin Cai
JC
Pan
Ci
C
Chen
Jing
J
Zhou
Xin
X
Wang
Yao Ping
YP
D000293:Adolescent; D000741:Anemia, Aplastic / Q000201:enzymology* / Q000473:pathology; D015415:Biomarkers; D001853:Bone Marrow / Q000201:enzymology / Q000473:pathology*; D001854:Bone Marrow Cells / Q000201:enzymology*; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D018450:Disease Progression; D005260:Female; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006801:Humans; D007223:Infant; D008297:Male; D009000:Monocytes / Q000201:enzymology*; D019098:Telomerase / Q000032:analysis*
D015415:Biomarkers; D019098:Telomerase