A simple method for the determination of ethylene-thiourea (ETU) in biological samples.
A direct, simple, and rapid high-performance liquid chromatographic method has been developed for the determination of ethylene-thiourea (ETU) in biological fluids. Samples were chromatographed on a Lichrosorb RP8 (5 pm) column after extraction with dichloromethane. The mobile phase was a mixture of hexane/isopropyl alcohol/ethyl alcohol (93:6:1 v/v) added with 0.6 mL/L butylamine. Detection was done with a UV detector set at 243 nm. This method was validated to standard criteria. Calibration curves for ETU in 100 microL of 0.9% saline, 500 microL plasma, and 10 mL urine were linear (r2 > 0.99) from 0.05 to 30 microg/mL, 0.025 to 30 microg/mL, and 1 to 100 ng/mL, respectively. The lower limit of detection was 20 ng/mL in plasma, 25 ng/mL in 0.9% saline, and 0.5 ng/mL in urine.
Debbarh
Ikram
I
Department of Pharmacology, Université Victor Segalen, Bordeaux, France.
Moore
Nicholas
N
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004784:Environmental Monitoring / Q000379:methods; D004787:Environmental Pollution / Q000032:analysis; D005031:Ethylenethiourea / Q000032:analysis* / Q000493:pharmacokinetics; D005659:Fungicides, Industrial / Q000378:metabolism; D006801:Humans; D008297:Male; D010573:Pesticide Residues / Q000032:analysis; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005659:Fungicides, Industrial; D010573:Pesticide Residues; D005031:Ethylenethiourea