title: Noradrenergic and cholinergic innervation of the bone marrow.
abstract: Bone marrow is supplied by sensory and autonomic innervation. Although it is well established that hematopoiesis is regulated by cytokines and cell-to-cell contacts, the role played by neuromediators on the proliferation, differentiation and release of hematopoietic cells is still controversial. We studied the innervation of rat femur bone marrow by means of fluorescence histochemistry and immunohistochemistry. Glyoxylic acid-induced fluorescence was used to demonstrate catecholaminergic nerve fibers. The immunoperoxidase method with nickel amplification was applied to detect the distribution of nerve fibers using antibodies against the general neuronal marker PGP 9.5 (neuron-specific cytoplasmic protein), while the cholinacetyltransferase immunoreactivity was studied by immunohistochemistry. Our results show the presence of an extensive network of innervation in the rat bone marrow, providing a morphological basis for the neural modulation of hemopoiesis.
authors.affiliation: Department of Pharmacology of Natural Molecules and General Physiology, University of Rome La Sapienza, Italy. marco.artico@uniroma1.it
authors.forename: Marco
authors.initials: M
authors.lastname: Artico
authors.forename: Sandro
authors.initials: S
authors.lastname: Bosco
authors.forename: Carlo
authors.initials: C
authors.lastname: Cavallotti
authors.forename: Enzo
authors.initials: E
authors.lastname: Agostinelli
authors.forename: Gabriella
authors.initials: G
authors.lastname: Giuliani-Piccari
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sciorio
authors.forename: Lucio
authors.initials: L
authors.lastname: Cocco
authors.forename: Marco
authors.initials: M
authors.lastname: Vitale
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000294:innervation* / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D005269:Femur; D007150:Immunohistochemistry; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002794:Choline; D009638:Norepinephrine