title: Analysis of the NRT2 nitrate transporter family in Arabidopsis. Structure and gene expression.
abstract: Nitrate is an essential element for plant growth, both as a primary nutrient in the nitrogen assimilation pathway and as an important signal for plant development. The uptake of nitrate from the soil and its translocation throughout the plant has been the subject of intensive physiological and molecular studies. Using a reverse genetic approach, the AtNRT2.1 gene has been shown to be involved in the inducible component of the high-affinity nitrate transport system in Arabidopsis. The Arabidopsis Genome Initiative has released nearly the whole genome sequence of Arabidopsis, allowing the identification of a small NRT2 multigene family in this species. Thus, we investigated the phylogenetic relationship between NRT2 proteins belonging to several kingdoms and compared the structure of the different members of the Arabidopsis family. We analyzed, by semiquantitative reverse transcriptase-polymerase chain reaction, the expression pattern of each gene depending on plant organ and development or nutritional status, and compared the relative level of each gene by real-time polymerase chain reaction. We also evaluated the significance of each paralog on the basis of the relative levels of gene expression. The results are discussed in relation with distinct roles for the individual members of the AtNRT2 family.
authors.affiliation: Unité de la Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique, Route de St. Cyr, F-78026 Versailles cedex, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Orsel
authors.forename: Anne
authors.initials: A
authors.lastname: Krapp
authors.forename: Françoise
authors.initials: F
authors.lastname: Daniel-Vedele
mesh_terms: D027321:Anion Transport Proteins / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D001693:Biological Transport, Active / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D005810:Multigene Family / Q000235:genetics; D000091285:Nitrate Transporters; D009566:Nitrates / Q000494:pharmacology; D010802:Phylogeny; D010940:Plant Proteins; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology
chemical_list: D027321:Anion Transport Proteins; D029681:Arabidopsis Proteins; D000091285:Nitrate Transporters; D009566:Nitrates; D010940:Plant Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C477335:NRT2.1 protein, Arabidopsis