Amyloid-beta-induced chemokine production in primary human macrophages and astrocytes.
In Alzheimer's disease (AD), chemotaxis might be responsible for attracting glial cells towards the neuritic plaque. Using primary monocyte-derived macrophages and primary adult astrocytes as a model, amyloid-beta (Abeta) (1-42) was able to stimulate the production, as measured by RT-PCR, of MIP-1alpha and MIP-1beta mRNA in macrophages and MCP-1 in astrocytes. Cocultures showed in unstimulated as well as in Abeta-stimulated cells an increase in MIP-1alpha, MIP-1beta and MCP-1 mRNA. ELISAs of supernatant samples of stimulated macrophages and astrocytes also showed an increase in MIP-1alpha and MIP-1beta in macrophages and MCP-1 in astrocytes. Stimulated cocultures showed an increase in MIP-1alpha, MIP-1beta and MCP-1 protein levels in contrast to unstimulated cocultures.
Smits
Hessel A
HA
Section Neuroimmunology, Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Center Utrecht, Heidelberglaan 100, NL-3584 CX Utrecht, The Netherlands.
Rijsmus
Annemarie
A
van Loon
Joyce H
JH
Wat
Jesse W Y
JW
Verhoef
Jan
J
Boven
Leonie A
LA
Nottet
Hans S L M
HS
D000328:Adult; D000544:Alzheimer Disease / Q000276:immunology; D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018925:Chemokines / Q000235:genetics*; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D018920:Coculture Techniques; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D006801:Humans; D019402:Macrophage Inflammatory Proteins / Q000235:genetics; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D010446:Peptide Fragments / Q000494:pharmacology*
D016229:Amyloid beta-Peptides; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018925:Chemokines; D019402:Macrophage Inflammatory Proteins; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42)