Accuracy of day 3 criteria for selecting the best embryos.
OBJECTIVE
To assess the accuracy of day 3 morphologic criteria in identifying the best embryos.


METHODS
Prospective observational study.


METHODS
University IVF program.


METHODS
One hundred cycles in women desiring blastocyst transfer who had > or =3 eight-cell embryos on day 3.


METHODS
On day 3, the embryologist chose the two embryos that would have been transferred that day. On day 5, embryos were examined to determine the best and second-best blastocysts.


METHODS
Accuracy of day 3 picks as measured in culture on day 5, outcome of nontransferred picks, and cryopreservation rate.


RESULTS
All cycles reached the blastocyst stage and 73% had cryopreservation. The mean number of blastocysts was 4.8 (3.2 on day 5 and 1.6 on day 6). Neither pick was chosen in 39% of cycles; one pick was transferred in 38%; and both picks were transferred in 23%. Of 116 nontransferred picks, 51 were frozen and 65 arrested, with both picks arresting in 9 cycles. The single best blastocyst was chosen from the picks in 39% of cycles.


CONCLUSIONS
Morphologic criteria for cleavage-stage embryo selection may fall short when the transfer is limited to two embryos. Culture to blastocyst is warranted in this population to avoid high-order multiples and still be able to choose the two embryos with the highest implantation potential.
Milki
Amin A
AA
Department of Gynecology and Obstetrics, Stanford University School of Medicine, Stanford, California, USA. milki@aol.com
Hinckley
Mary D
MD
Gebhardt
Janice
J
Dasig
Daniel
D
Westphal
Lynn M
LM
Behr
Barry
B
D000328:Adult; D001755:Blastocyst / Q000502:physiology; D002970:Cleavage Stage, Ovum; D015925:Cryopreservation; D046508:Culture Techniques; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D006801:Humans; D011446:Prospective Studies; D013997:Time Factors