Polyamine uptake is necessary for a normal biochemical maturation of astrocytes in culture.
In this study we have explored the importance of polyamine uptake in the proliferation and biochemical maturation of cerebellar astroyctes in culture. The uptake of polyamines paralleled astrocyte proliferation measured as [3H]thymidine incorporation into the DNA. Inhibition of polyamine uptake did not alter the developmental profile of thymidine incorporation, perhaps due to a compensatory increase in ornithine decarboxylase activity but was able to reduce glutamine synthetase (GS) activity, an enzymatic marker for astrocyte biochemical maturation, from 9 days in vitro. The present results suggest that polyamine uptake plays an important role in the biochemical maturation of astrocytes in culture.
Dot
Josep
J
Instituto de Neurociencias and Departamento de Bioquímica y Biología Molecular, Edificio M, Universidad Autónoma de Barcelona,08193 Cerdanyola del Vallés, Barcelona, Spain.
Danchev
Nikolai
N
Blanco
Isaac
I
Rodríguez-Alvarez
José
J
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000201:enzymology*; D015317:Biogenic Polyamines / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D004247:DNA / Q000096:biosynthesis; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D000518:Eflornithine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D051379:Mice; D008935:Mitoguazone / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology; D013095:Spermidine / Q000378:metabolism / Q000494:pharmacology; D013096:Spermine / Q000378:metabolism / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D015317:Biogenic Polyamines; D004791:Enzyme Inhibitors; D013096:Spermine; D004247:DNA; D009955:Ornithine Decarboxylase; D005974:Glutamate-Ammonia Ligase; D008935:Mitoguazone; D013095:Spermidine; D011700:Putrescine; D013936:Thymidine; D000518:Eflornithine