title: A broad host range plasmid vector that does not encode replication proteins.
abstract: The 640-bp minimal replication region derived from a plasmid DNA preparation from an Acidothiobacillus ferrooxidans strain capable of autonomous replication in a range of Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Acinetobacter calcoaceticus and Alcaligenes faecalis) was identified. This DNA fragment (named TFK replicon) does not encode Rep proteins and appears to be unrelated to other known replicons.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, 123182, Moscow, Russia.
authors.forename: Eza
authors.initials: E
authors.lastname: Kalyaeva
authors.forename: Irina
authors.initials: I
authors.lastname: Bass
authors.forename: Gennady
authors.initials: G
authors.lastname: Kholodii
authors.forename: Vadim
authors.initials: V
authors.lastname: Nikiforov
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004261:DNA Replication / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012093:Replicon / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial