title: Effects of Coriolus versicolor polysaccharide B on monocyte chemoattractant protein 1 gene expression in rat.
abstract: OBJECTIVE
  To investigate the effect of Coriolus versicolor polysaccharide B (CVPS-B), a new water-soluble component of polysaccharides from the fungus Coriolus versicolor (Fr) L on monocyte chemoattractant protein-1 (MCP-1) gene expression in rat splenocytes.
  
  
  METHODS
  Expression of MCP-1 mRNA in rat splenocytes was examined by reverse transcription-polymerase chain reaction (RT-PCR) with beta- actin as an internal standard. Sequencing of RT-PCR products was performed to confirm their specificity in MCP-1 gene composition.
  
  
  RESULTS
  (1) Without pre-treatment of lipopolysaccharide (LPS), the relative MCP-1 mRNA expression ratios (MCP-1/beta-actin) for the saline control group and for CVPS-B groups in 3 different doses (10, 20, and 30 mg . kg-1 . d-1, ip, for 4 d) were 1.4 +/- 0.3, 1.6 +/- 0.4, 1.7 +/- 0.5, and 1.5 +/- 0.4, respectively (P > 0.05); (2) LPS (10 microg . kg-1, ip) enhanced the expression of MPC-1 mRNA by the ratio of 114 %; (3) pre-treatment with CVPS-B of 4 different doses (5, 10, 30, and 50 mg . kg-1 . d-1, ip, for 4 d) decreased the LPS induced expression of MPC-1 mRNA by the ratios of 51 %, 70 %, 84 %, and 99 %, respectively (n = 6).
  
  
  CONCLUSIONS
  In a dose-related fashion, CVPS-B inhibited the expression of MCP-1 mRNA induced by LPS in the rat splenocytes, but did not significantly affect the expression of MPC-1 mRNA in the normal rat.
authors.affiliation: Laboratory for Molecular Pharmacology, Department of Hygiology, First Military Medical University, Guangzhou 510515, China. songlc@fimmu.edu.cn
authors.forename: Lie-Chang
authors.initials: LC
authors.lastname: Song
authors.forename: Hai-Sheng
authors.initials: HS
authors.lastname: Chen
authors.forename: Ning
authors.initials: N
authors.lastname: Lou
authors.forename: Chang
authors.initials: C
authors.lastname: Song
authors.forename: Jun
authors.initials: J
authors.lastname: Zeng
authors.forename: Ting-Huan
authors.initials: TH
authors.lastname: Fu
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D020072:Polyporales / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000378:metabolism
chemical_list: D018932:Chemokine CCL2; D008070:Lipopolysaccharides; D011134:Polysaccharides; D012333:RNA, Messenger