[Molecular biological identification of Cuninghamella spec].
Among the genus Cunninghamella, so far C. bertholletiae is known to be the only clinically relevant species. Correct identification of C. bertholletiae is not possible with classical methods. PCR and sequencing of the internal transcribed spacer (ITS) region was used to identify seven of nine clinical isolates as C. bertholletiae and two as C. echinulata. Also an isolate of the surrounding area of one patient infected with C. echinulata could be identified as C. echinulata. High homology in the ITS region was found within the isolates of C. bertholletiae. Within the species C. echinulata and C. elegans a differentiation on subspecies level was achieved by an analysis of restriction fragment length polymorphism of the ITS amplicons after incubation with TaqI and HinfI. Similar results were obtained by PCR fingerprinting of the complete DNA with the single microsatellite DNA primers (GTG)5 and (GAC)5. For the first time C. echinulata could be identified as agent of zygomycosis in humans.
Lemmer
Karin
K
Robert Koch-Institut, Mykologie, Nordufer 20, D-13353 Berlin, Germany. lemmerk@rki.de
Losert
Heidemarie
H
Rickerts
V
V
Just-Nübling
Gudrun
G
Sander
Anna
A
Kerkmann
Marie-Louise
ML
Tintelnot
Kathrin
K
D001483:Base Sequence; D020093:Cunninghamella / Q000145:classification / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D004271:DNA, Fungal; D021901:DNA, Intergenic
Molekularbiologische Identifizierung von Cunninghamella spec.