rugose (rg), a Drosophila A kinase anchor protein, is required for retinal pattern formation and interacts genetically with multiple signaling pathways.
In the developing Drosophila eye, cell fate determination and pattern formation are directed by cell-cell interactions mediated by signal transduction cascades. Mutations at the rugose locus (rg) result in a rough eye phenotype due to a disorganized retina and aberrant cone cell differentiation, which leads to reduction or complete loss of cone cells. The cone cell phenotype is sensitive to the level of rugose gene function. Molecular analyses show that rugose encodes a Drosophila A kinase anchor protein (DAKAP 550). Genetic interaction studies show that rugose interacts with the components of the EGFR- and Notch-mediated signaling pathways. Our results suggest that rg is required for correct retinal pattern formation and may function in cell fate determination through its interactions with the EGFR and Notch signaling pathways.
Shamloula
Hoda K
HK
Department of Biology, City College and The Graduate Center, City University of New York, New York 10031, USA.
Mbogho
Mkajuma P
MP
Pimentel
Angel C
AC
Chrzanowska-Lightowlers
Zosia M A
ZM
Hyatt
Vanneta
V
Okano
Hideyuki
H
Venkatesh
Tadmiri R
TR
D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D048049:Extracellular Signal-Regulated MAP Kinases; D005136:Eye Proteins / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics; D018773:Genes, erbB-1; D008565:Membrane Proteins / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D017956:Photoreceptor Cells, Invertebrate / Q000196:embryology* / Q000378:metabolism; D012160:Retina / Q000196:embryology; D015398:Signal Transduction / Q000502:physiology*; D018631:ras Proteins
D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005136:Eye Proteins; D008565:Membrane Proteins; C415248:S protein, Drosophila; C108769:rg protein, Drosophila; C107990:sv protein, Drosophila; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048049:Extracellular Signal-Regulated MAP Kinases; C086795:rl protein, Drosophila; D019204:GTP-Binding Proteins; C417283:Ras85D protein, Drosophila; D018631:ras Proteins