Births of calves derived from embryos produced by intracytoplasmic sperm injection without exogenous oocyte activation.
Tail-cut bovine spermatozoa were microinjected into ooplasmic lipid polarised, in vitro matured bovine oocytes using a piezomicropipette-driving system. No exogenous oocyte activation treatment was used. Of the sperm-injected oocytes, 86.3% were activated, 71.8% cleaved and 22.7% developed to the blastocyst stage. The average cell count of the blastocysts was 122.5 +/- 15 and a majority (81.8%) of the blastocysts were cytologically normal (diploid). When transferred to recipient cows, 5 of 8 blastocysts developed to fetuses and 4 of 7 recipients became pregnant. Normal offspring were born.
Wei
Hong
H
Laboratory of Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Fukui
Yukata
Y
D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002417:Cattle; D005260:Female; D008297:Male; D009865:Oocytes / Q000502:physiology; D011205:Povidone / Q000378:metabolism; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000662:veterinary; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods / Q000662:veterinary*
D011205:Povidone