title: Automatic potentiometric flow titration procedure for ascorbic acid determination in pharmaceutical formulations.
abstract: A flow procedure for the determination of ascorbic acid in pharmaceutical formulations exploiting potentiometric titration is described. The method is based on the reduction of IO3- by ascorbic acid and the detection was carried out employing a flow-through ion selective electrode for iodide. The flow network controlled by a microcomputer was designed to implement multicommutation for ease of operation and robustness. The titration system allowed the determination of ascorbic acid in pharmaceutical formulations with concentrations ranging from 7.5 to 15.0 mmol l(-1). No significant differences at the 95% confidence level were observed in comparison with results obtained by a manual procedure. Merit figures of results such as a relative standard deviation of 1.0% (n=6) and a reagent consumption of 21.4 mg IO3- per determination were obtained.
authors.affiliation: Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Av. Centenário 303, P.O. Box 96, 13400-970 Piracicaba, São Paulo, Brazil.
authors.forename: Ana Paula S
authors.initials: AP
authors.lastname: Paim
authors.forename: Cristina M N V
authors.initials: CM
authors.lastname: Almeida
authors.forename: Boaventura F
authors.initials: BF
authors.lastname: Reis
authors.forename: Rui A S
authors.initials: RA
authors.lastname: Lapa
authors.forename: Elias A G
authors.initials: EA
authors.lastname: Zagatto
authors.forename: José L F
authors.initials: JL
authors.lastname: Costa Lima
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D002626:Chemistry, Pharmaceutical; D016166:Free Radical Scavengers / Q000032:analysis*; D011199:Potentiometry / Q000379:methods*
chemical_list: D016166:Free Radical Scavengers; D001205:Ascorbic Acid