title: Investigating the tolerance of coiled-coil peptides to nonheptad sequence inserts.
abstract: Coiled-coil motifs foster a wide variety of protein-protein interactions. Canonical coiled coils are based on 7-residue repeats, which guide the folding and assembly of amphipathic alpha-helices. In many cases such repeats remain unbroken for tens to hundreds of residues. However, the sequences of an increasing number of putative and characterised coiled coils digress from this pattern. We probed the consequences of nonheptad inserts using a designed leucine-zipper system. The parent peptide, SKIP0, which had four contiguous heptads, was confirmed as a parallel homodimer by circular dichroism spectroscopy and analytical ultracentrifugation. Seven daughter peptides were constructed in which 1 to 7 alanine residues were inserted between the central heptads of SKIP0. Like SKIP0, SKIP7 formed a stable helical dimer, but the other peptides were highly destabilised, with the order of dimer stability SKIP4 >> SKIP5 > SKIP6 > SKIP3 > SKIP2 > SKIP1. These results are consistent with an extended theory of coiled-coil assembly in which coiled-coil-compatible motifs are based on 3- and 4-residue spacings and most notably heptad (7-residue) and hendecad (11-residue) repeats. Thus, they help explain why in natural sequences, inserts after canonical heptad repeats most commonly comprise 4 residues. Possible biological roles for nonheptad inserts are discussed.
authors.affiliation: Centre for Biomolecular Design and Drug Development, School of Biological Sciences, University of Sussex, Falmer, BN1 9QG, United Kingdom.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Hicks
authors.forename: John
authors.initials: J
authors.lastname: Walshaw
authors.forename: Derek N
authors.initials: DN
authors.lastname: Woolfson
mesh_terms: D000409:Alanine / Q000737:chemistry; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001703:Biophysics / Q000379:methods*; D002942:Circular Dichroism; D019281:Dimerization; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013696:Temperature; D014461:Ultracentrifugation
chemical_list: D010455:Peptides; D000409:Alanine