Identification of the regulatory region of the peripheral myelin protein 22 (PMP22) gene that directs temporal and spatial expression in development and regeneration of peripheral nerves.
Minor changes in PMP22 gene dosage have profound effects on the development and maintenance of peripheral nerves. This is evident from the genetic disease mechanisms in Charcot-Marie-Tooth disease type 1A (CMT1A) and hereditary neuropathy with liability to pressure palsies (HNPP) as well as transgenic animals with altered PMP22 gene dosage. Thus, regulation of PMP22 is a crucial aspect in understanding the function of this protein in health and disease. In this study, we have generated transgenic mice containing 10 kb of the 5'-flanking region of the PMP22 gene, including the two previously identified alternative promoters, fused to a lacZ reporter gene. We show that this part of the PMP22 gene contains the necessary information to mirror the endogenous expression pattern in peripheral nerves during development and regeneration and in mouse models of demyelination due to genetic lesions. Transgene expression is strongly regulated during myelination, demyelination, and remyelination in Schwann cells, demonstrating the crucial influence of neuron-Schwann cell interactions in the regulation of PMP22. In addition, the region of the PMP22 gene present on this transgene confers also neuronal expression in sensory and motor neurons. These results provide the crucial basis for further dissection of the elements that direct the temporal and spatial regulation of the PMP22 gene and to elucidate the molecular basis of the master program regulating peripheral nerve myelination.
Maier
Marcel
M
Institute of Cell Biology, Department of Biology, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zurich, Switzerland.
Berger
Philipp
P
Nave
Klaus-Armin
KA
Suter
Ueli
U
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D005260:Female; D005333:Fetus; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005809:Genes, Regulator / Q000502:physiology*; D017930:Genes, Reporter / Q000502:physiology; D007763:Lac Operon / Q000502:physiology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009185:Myelin Proteins / Q000096:biosynthesis / Q000235:genetics*; D009186:Myelin Sheath / Q000378:metabolism* / Q000648:ultrastructure; D009413:Nerve Fibers, Myelinated / Q000378:metabolism* / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000235:genetics*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism; D010525:Peripheral Nerves / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000502:physiology; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism; D019076:Transgenes / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D014781:Viscera / Q000166:cytology / Q000378:metabolism; D014855:Wallerian Degeneration / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D009185:Myelin Proteins; C489545:Pmp22 protein, mouse; D001616:beta-Galactosidase