title: Charaterization of Leishmania major Friedlin telomeric terminus.
abstract: Here we have characterized Leishmania major (Friedlin) telomeric terminus (the very end) using recombinants obtained by a vector-adaptor cloning protocol. As in L. donovani, the last nine nucleotides of L. major terminus are 5'-GGTTAGGGT-OH 3', differing from Trypanosoma cruzi and T. brucei terminus 5'GGGTTAGGG-OH 3', thus indicating that these sequences are genus specific. We have also made a comparative analysis between L. major and L. donovani telomere-associated sequences, and described a novel non-repeated telomeric associated sequence common to L. major low molecular weight chromosomal bands.
authors.affiliation: Laboratorio de Genetica Molecular, Instituto de Biología Experimental, Universidad Central de Venezuela, Caracas, Venezuela.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Chiurillo
authors.forename: José Luis
authors.initials: JL
authors.lastname: Ramírez
mesh_terms: D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D007893:Leishmania donovani / Q000235:genetics; D018320:Leishmania major / Q000235:genetics*; D008969:Molecular Sequence Data; D016615:Telomere / Q000737:chemistry / Q000235:genetics*