Mediators of peripheral blood neutrophilia induced by photodynamic therapy of solid tumors.
Photodynamic therapy (PDT) of tumors elicits a strong host immune response and one of its manifestations is a pronounced neutrophilia. By blocking their function prior to Photofrin-based PDT of mouse EMT6 tumors, we have identified multiple mediators whose regulated action is responsible for this neutrophilia. In addition to complement fragments (direct mediators) released as a consequence of PDT-induced complement activation, there are at least a dozen secondary mediators that all arise as a result of complement activity. The latter include cytokines IL-1beta, TNF-alpha, IL-6, IL-10, G-CSF and KC, thromboxane, prostaglandins, leukotrienes, histamine, and coagulation factors.
Cecic
Ivana
I
Cancer Imaging Department, BC Cancer Research Centre, 601 West 10th Avenue, BC V5Z 1L3, Vancouver, Canada.
Korbelik
Mladen
M
D000818:Animals; D017323:Dihematoporphyrin Ether / Q000009:adverse effects / Q000627:therapeutic use*; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D009504:Neutrophils / Q000187:drug effects*; D010778:Photochemotherapy / Q000009:adverse effects*; D012513:Sarcoma, Experimental / Q000188:drug therapy* / Q000473:pathology; D013997:Time Factors
D017323:Dihematoporphyrin Ether