A phosphate-stimulated NAD(P)+-dependent glyceraldehyde-3-phosphate dehydrogenase in Bacillus cereus.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme of central carbon metabolism, was studied in a Bacillus cereus strain isolated from the phosphate layer from Morocco. Enzymatic assays with cell extracts demonstrated that when grown on Luria-Bertani (LB) medium, B. cereus contains a major NAD+-dependent GAPDH activity and only traces of NADP+-dependent activity, but in cells grown on Pi-supplemented LB medium a strong increase of the NADP+-dependent activity, that became predominant, occurs concurrently with a GAPDH protein increase. Our results show that B. cereus possesses two GAPDH activities, namely NAD+- and NADP+-dependent, catalyzed by two enzymes with distinct coenzyme specificity and different phosphate regulation patterns. The finding of a phosphate-stimulated NADP+-dependent GAPDH in B. cereus indicates that this bacterium can modulate its primary carbon metabolism according to phosphate availability.
Iddar
Abdelghani
A
Laboratoire de Biochimie, Département de Biologie, Facultés des Sciences Aïn-Chock, Université Hassan-II, Km 8 route d'El Jadida, B.P. 5366 Mâarif, Casablanca, Morocco.
Serrano
Aurelio
A
Soukri
Abdelaziz
A
D001409:Bacillus cereus / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D015153:Blotting, Western; D002244:Carbon / Q000378:metabolism; D002384:Catalysis; D004789:Enzyme Activation / Q000187:drug effects; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010710:Phosphates / Q000494:pharmacology*
D010710:Phosphates; D009243:NAD; D009249:NADP; D002244:Carbon; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases