title: Coherent and robust modulation of a metabolic network by cytoskeletal organization and dynamics.
abstract: In order to investigate the influence of cytoskeletal organization and dynamics on cellular biochemistry, a mathematical model was formulated based on our own experimental evidence. The model couples microtubular protein (MTP) dynamics to the glycolytic pathway and its branches: the Krebs cycle, ethanolic fermentation, and the pentose phosphate (PP) pathway. Results show that the flux through glycolysis coherently and coordinately increases or decreases with increased or decreased levels of polymerized MTP, respectively. The rates of individual enzymatic steps and metabolite concentrations change with the polymeric status of MTP throughout the metabolic network. Negative control is exerted by the PP pathway on the glycolytic flux, and the extent of inhibition depends inversely on the polymerization state of MTP, i.e. a high degree of polymerization relieves the negative control. The stability of the model's steady state dynamics for a wide range of variation of metabolic parameters increased with the degree of polymerized MTP. The findings indicate that the organization of the cytoskeleton bestows coherence and robustness to the coordination of cellular metabolism.
authors.affiliation: Instituto Tecnológico de Chascomús (INTECH/CONICET), Casilla de Correo 164, 7130- Chascomús, Buenos Aires, Argentina. maon@bme.jhu.edu
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Aon
authors.forename: Sonia
authors.initials: S
authors.lastname: Cortassa
mesh_terms: D003599:Cytoskeleton / Q000378:metabolism*; D004795:Enzyme Stability; D005988:Glyceric Acids / Q000378:metabolism; D006019:Glycolysis; D008870:Microtubules / Q000378:metabolism; D008962:Models, Theoretical; D010427:Pentose Phosphate Pathway / Q000502:physiology*
chemical_list: D005988:Glyceric Acids