title: Size characterization of barley starch granules by gravitational field-flow fractionation: a rapid, low-cost method to assess the brewing capability of different strains.
abstract: Cereal starch occurs as two types of micrometer-sized granules, large and small. Large starch granules are more susceptible to enzymatic hydrolysis. When cereal starch is used for fermentation processes, as in brewing of barley malt, the barley strains with the highest content of large starch granules should be preferred. Gravitational field-flow fractionation (GFFF) is a separation method able to fractionate starch samples at low cost and short analysis time. In this work, the search for the best GFFF conditions for the analytical separation of barley starch within an inter-laboratory approach is presented. For different barley strains cultivated under monitored conditions the size distributions of starch granules is here quickly monitored and characterized by GFFF. As a consequence, dimensional characterization of barley starch can allow for the selection of the most suitable strains with the lowest content of non-degradable starch.
authors.affiliation: Department of Chemistry G. Ciamician, University of Bologna, Via Selmi 2, 40126 Bologna, Italy. resky@ciam.unibo.it
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Reschiglian
authors.forename: Andrea
authors.initials: A
authors.lastname: Zattoni
authors.forename: Sonia
authors.initials: S
authors.lastname: Casolari
authors.forename: Andrea
authors.initials: A
authors.lastname: Krumlova
authors.forename: Marcela
authors.initials: M
authors.lastname: Budinska
authors.forename: Josef
authors.initials: J
authors.lastname: Chmelík
mesh_terms: D005285:Fermentation; D031521:Fractionation, Field Flow; D001467:Hordeum / Q000737:chemistry* / Q000502:physiology; D010316:Particle Size; D013213:Starch / Q000032:analysis / Q000737:chemistry*
chemical_list: D013213:Starch