title: Environmental exposure to polychlorinated biphenyls and placental CYP1A1 activity in Inuit women from northern Québec.
abstract: Some polychlorinated biphenyl (PCB) congeners are CYP1A1 inducers, and induction of this enzyme in the placenta has been linked to adverse effects on fetal development. The objective of this study was to determine if the body burden of PCBs is related to placental CYP1A1 activity in Inuit women from Nunavik (northern Québec), a population highly exposed to organochlorines. Placenta and cord blood samples were obtained from 35 Inuit women and 30 women from a southern Québec community exposed to background levels of organochlorines. We measured PCB concentrations in all cord plasma samples and in a subset of placenta samples from the Nunavik group and assessed CYP1A1 activity (ethoxyresorufin-O-deethylase; EROD) in placental microsomes from all participants. Concentrations of PCBs in cord plasma were strongly correlated to those in placenta (Pearson's r) = 0.77-0.97, p < 0.001) and were on average 4-fold higher in Inuit women than in southern Québec women [for PCB 153, the geometric means (geometric SDs) were 83.3 (1.9) ng/g lipid vs. 16.9 (1.6) ng/g lipid, respectively]. Despite this difference in PCB body burden, both study groups had similar EROD activities when data were stratified according to tobacco smoking. Although simple correlation analysis first showed that placental EROD activity was correlated with PCB 153 plasma concentration in the Nunavik group, a multivariate analysis failed to demonstrate a significant contribution of PCBs to EROD activity when tobacco smoking was included in the analysis. We conclude that dietary exposure to PCBs in Inuit women from Nunavik does not significantly influence EROD activity in the placenta, implicating tobacco smoking as the major modulating factor.
authors.affiliation: Département de Biologie Médicale, Faculté de Médecine, Université Laval et Unité de recherche en santé et environnement, Ste-Foy, Québec, Canada.
authors.forename: Daria
authors.initials: D
authors.lastname: Pereg
authors.forename: Eric
authors.initials: E
authors.lastname: Dewailly
authors.forename: Guy G
authors.initials: GG
authors.lastname: Poirier
authors.forename: Pierre
authors.initials: P
authors.lastname: Ayotte
mesh_terms: D000328:Adult; D001822:Body Burden; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis / Q000096:biosynthesis*; D004032:Diet; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000009:adverse effects* / Q000493:pharmacokinetics; D004790:Enzyme Induction; D016021:Epidemiologic Studies; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D006801:Humans; D007198:Indians, North American; D010920:Placenta / Q000187:drug effects / Q000201:enzymology*; D011078:Polychlorinated Biphenyls / Q000009:adverse effects* / Q000493:pharmacokinetics; D011247:Pregnancy; D011792:Quebec / Q000453:epidemiology; D012907:Smoking / Q000009:adverse effects
chemical_list: D004785:Environmental Pollutants; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1