Monocyte chemotactic protein-1 levels in the vitreous of patients with proliferative vitreoretinopathy.
OBJECTIVE
To assess the potential role of monocyte chemotactic protein-1 (MCP-1) in the pathogenesis of proliferative vitreoretinopathy (PVR) and to investigate its possible interaction with the macrophage migration inhibitory factor (MIF).


METHODS
We assayed MCP-1 and MIF levels in the vitreous samples of 85 consecutive patients with PVR (29 eyes), rhegmatogenous retinal detachment (RRD; 22 eyes), and macular hole or idiopathic epimacular membrane (controls; 34 eyes), by enzyme-linked immunosorbent assay.


RESULTS
Vitreous levels of MCP-1 were 1760.7 +/- 471.3 pg/mL (mean +/- SD) in PVR patients, 1200.4 +/- 579.8 pg/mL in RRD patients, and 436.3 +/- 286.1 pg/mL in the controls. Vitreous MCP-1 levels in PVR patients were significantly higher than those in RRD patients and in the controls (P <.0001, respectively). MCP-1 levels in grade C of PVR (1883.7 +/- 479.5 pg/mL) were significantly greater than those in grade D (1437.8 +/- 258.8 pg/mL) (P =.0112). Vitreous concentrations of MCP-1 had no correlation with those of MIF.


CONCLUSIONS
The results indicate the possibility that MCP-1 may have a role mainly in the early stage of PVR and that the role of MCP-1 in PVR may differ from that of MIF.
Mitamura
Yoshinori
Y
Department of Ophthalmology, Sapporo Medical University School of Medicine, Sapporo, Japan.
Takeuchi
Shinobu
S
Yamamoto
Shuichi
S
Yamamoto
Teiko
T
Tsukahara
Itsuro
I
Matsuda
Akira
A
Tagawa
Yoshitsugu
Y
Mizue
Yuka
Y
Nishihira
Jun
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018932:Chemokine CCL2 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D019773:Epiretinal Membrane / Q000378:metabolism; D005260:Female; D006801:Humans; D008263:Macrophage Migration-Inhibitory Factors / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012163:Retinal Detachment / Q000378:metabolism; D012167:Retinal Perforations / Q000378:metabolism; D018630:Vitreoretinopathy, Proliferative / Q000378:metabolism*; D014822:Vitreous Body / Q000378:metabolism*
D018932:Chemokine CCL2; D008263:Macrophage Migration-Inhibitory Factors