title: Effects of endurance training on activity and expression of AMP-activated protein kinase isoforms in rat muscles.
abstract: The effects of endurance training on the response of muscle AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) to moderate treadmill exercise were examined. In red quadriceps, there was a large activation of alpha 2-AMPK and inactivation of ACC in response to exercise. This response was greatly reduced after training, probably because of reduced metabolic stress. In white quadriceps, there were no effects of exercise on AMPK or ACC, but alpha 2-activity was higher after training because of increased phosphorylation of Thr(172). In soleus, there were small increases in alpha 2-activity during exercise that were not affected by training. The expression of all seven AMPK subunit isoforms was also examined. The beta 2- and gamma 2-isoforms were most highly expressed in white quadriceps, and gamma 3 was expressed in red quadriceps and soleus. There was a threefold increase in expression of gamma 3 after training in red quadriceps only. Our results suggest that gamma 3 might have a special role in the adaptation to endurance exercise in muscles utilizing oxidative metabolism.
authors.affiliation: Division of Molecular Physiology, School of Life Sciences, Dundee University, Wellcome Trust Biocentre, Dundee, DD1 5EH Scotland, UK.
authors.forename: Paula E
authors.initials: PE
authors.lastname: Durante
authors.forename: Kirsty J
authors.initials: KJ
authors.lastname: Mustard
authors.forename: Soo-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: William W
authors.initials: WW
authors.lastname: Winder
authors.forename: D Grahame
authors.initials: DG
authors.lastname: Hardie
mesh_terms: D055372:AMP-Activated Protein Kinases; D000818:Animals; D015153:Blotting, Western; D004789:Enzyme Activation / Q000502:physiology; D006003:Glycogen / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D009043:Motor Activity; D009097:Multienzyme Complexes / Q000378:metabolism*; D018656:Muscle Fibers, Fast-Twitch / Q000201:enzymology; D018482:Muscle, Skeletal / Q000166:cytology / Q000201:enzymology*; D005082:Physical Exertion / Q000502:physiology*; D011233:Precipitin Tests; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D021122:Protein Subunits; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007527:Isoenzymes; D009097:Multienzyme Complexes; D021122:Protein Subunits; D006003:Glycogen; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases