title: Lectin as a marker for staining and purification of embryonic pancreatic epithelium.
abstract: The embryonic pancreatic epithelium, and later the ductal epithelium, is known to give rise to the endocrine and exocrine cells of the developing pancreas, but no specific surface marker for these cells has been identified. Here, we utilized Dolichos Biflorus Agglutinin (DBA) as a specific marker of these epithelial cells in developing mouse pancreas. From the results of an immunofluorescence study using fluorescein-DBA and pancreatic specific cell markers, we found that DBA detects specifically epithelial, but neither differentiating endocrine cells nor acinar cells. We further applied this marker in an immunomagnetic separation system (Dynabead system) to purify these putative multi-potential cells from a mixed developing pancreatic cell population. This procedure could be applied to study differentiation and cell lineage selections in the developing pancreas, and also may be applicable to selecting pancreatic precursor cells for potential cellular engineering.
authors.affiliation: Laboratory of Surgical Organogenesis, Children's Mercy Hospital, 2401 Gillham Road, Kansas City, MO 64108, USA.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Troy L
authors.initials: TL
authors.lastname: Spilde
authors.forename: Zhixing
authors.initials: Z
authors.lastname: Li
authors.forename: Julie K
authors.initials: JK
authors.lastname: Marosky
authors.forename: Amina M
authors.initials: AM
authors.lastname: Bhatia
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Hembree
authors.forename: Krishna
authors.initials: K
authors.lastname: Prasadan
authors.forename: Barry L
authors.initials: BL
authors.lastname: Preuett
authors.forename: George K
authors.initials: GK
authors.lastname: Gittes
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002478:Cells, Cultured; D004848:Epithelium / Q000737:chemistry / Q000196:embryology; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D018189:Immunomagnetic Separation / Q000379:methods*; D037102:Lectins / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D010179:Pancreas / Q000737:chemistry / Q000166:cytology* / Q000196:embryology*; D010183:Pancreatic Ducts / Q000166:cytology / Q000196:embryology; D037121:Plant Lectins
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D037102:Lectins; D037121:Plant Lectins; C036554:dolichos biflorus agglutinin