RopGAP4-dependent Rop GTPase rheostat control of Arabidopsis oxygen deprivation tolerance.
Transient soil flooding limits cellular oxygen to roots and reduces crop yield. Plant response to oxygen deprivation involves increased expression of the alcohol dehydrogenase gene (ADH) and ethanolic fermentation. Disruption of the Arabidopsis gene that encodes Rop (RHO-like small G protein of plants) guanosine triphosphatase (GTPase) activating protein 4 (ROPGAP4), a Rop deactivator, elevates ADH expression in response to oxygen deprivation but decreases tolerance to stress. Rop-dependent production of hydrogen peroxide via a diphenylene iodonium chloride-sensitive calcium-dependent reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is necessary for induction of both ADH and RopGAP4 expression. Tolerance to oxygen deprivation requires Rop activation and RopGAP4-dependent negative feedback regulation. This Rop signal transduction rheostat balances the ability to increase ethanolic fermentation with survival.
Baxter-Burrell
Airica
A
Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA.
Yang
Zhenbiao
Z
Springer
Patricia S
PS
Bailey-Serres
Julia
J
D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004251:DNA Transposable Elements; D025461:Feedback, Physiological; D018506:Gene Expression Regulation, Plant; D006160:Guanosine Triphosphate / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D009861:Onium Compounds / Q000494:pharmacology; D018384:Oxidative Stress; D010100:Oxygen / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D015290:Second Messenger Systems; D015398:Signal Transduction; D014158:Transcription, Genetic; D020741:rho GTP-Binding Proteins / Q000378:metabolism*
D029681:Arabidopsis Proteins; D004251:DNA Transposable Elements; D009861:Onium Compounds; D012333:RNA, Messenger; D018749:RNA, Plant; D002110:Caffeine; C007517:diphenyleneiodonium; D006160:Guanosine Triphosphate; D006861:Hydrogen Peroxide; D000426:Alcohol Dehydrogenase; D019255:NADPH Oxidases; C459444:RopGAP4 protein, Arabidopsis; D020741:rho GTP-Binding Proteins; D010100:Oxygen; D002118:Calcium