title: Endogenous expression of nNOS protein in several neuronal cell lines.
abstract: Several neuronal cell lines were screened for endogenous expression of neuronal nitric oxide synthase (nNOS) protein using Western blot analysis. Detectable levels of the nNOS protein were evident in the SK-N-SH, SH-SY5Y, and N1E-115 neuroblastoma cell lines, as well as the NG108-15 neuroblastoma x glioma hybrid. Only trace amounts were visible in Neuro2A human neuroblastoma cells. The presence of endogenously expressed nNOS in these cells may allow for the study of the interaction between nNOS and the endogenous receptor systems expressed in the same cells.
authors.affiliation: Neuroscience Research in Psychiatry, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
authors.forename: Marianne K O
authors.initials: MK
authors.lastname: Grant
authors.forename: Adolfo E
authors.initials: AE
authors.lastname: Cuadra
authors.forename: Esam E
authors.initials: EE
authors.lastname: El-Fakahany
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism; D009474:Neurons / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D052248:Nitric Oxide Synthase Type I; D014407:Tumor Cells, Cultured
chemical_list: C496320:NOS1 protein, human; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I