Acidophiles of saline water at thermal vents of Vulcano, Italy.
DNA was extracted from samples taken from close to acidic hydrothermal vents on shore of the Aeolian Island of Vulcano (Italy). RNA gene sequences were amplified by PCR, cloned, and sequenced. A sequence with an origin in samples at 35 degrees and 45 degrees C corresponded to that of a novel Acidithiobacillus species that was isolated from water close to the vents. Novel, iron-oxidizing mesophilic acidophiles were isolated through enrichment cultures with ferrous iron but were not represented in the clone banks of environmental rDNA. These acidophiles were related to Thiobacillus prosperus, which was isolated previously from Vulcano. The archaeal sequences that comprised a clone bank representing a high-temperature sample (75 degrees C) corresponded to those of Acidianus brierleyi and of thermophiles previously isolated from Vulcano, Thermoplasma volcanium and Acidianus infernus.
Simmons
Susan
S
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
Norris
R
R
D001419:Bacteria / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D003470:Culture Media; D017931:DNA Primers; D006358:Hot Temperature; D007558:Italy; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride; D014871:Water Microbiology
D003470:Culture Media; D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride