title: [Value of the leukotriene generation test in diagnosis of immediate hypersensitivity to penicillin].
abstract: In this study we have decided to explore the diagnostic usefulness of CAST-ELISA test in patients with immediate allergic reactions caused by penicillin. Peripheral blood leukocytes were stimulated by commercially available penicillin allergens--Penicillin G, Benzyl-penicillin--Polylysine and specially performed Penicillin G conjugated to human serum albumin. Test was performed in 33 allergic persons and in 13 control healthy persons well tolerated penicillin antibiotics with negative skin tests results. CAST-ELISA test gave the positive results in 30.3% when unconjugated penicillin was used and in 18.18% with penicillin--polylysine. The best results were obtained when cells were stimulated with penicillin conjugated to human serum albumin--positive results were observed in 47.82%. Accumulate percentage of positive results was 73.91%. In our opinion CAST-ELISA test is reliable method in diagnosis of immediate allergic reactions caused by penicillin and inclusion of penicillin conjugated to human serum albumin seems to be justifiable.
authors.affiliation: Katedra i Klinika Chorób Wewnetrznych i Alergologii Akademii Medycznej we Wrocławiu.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Medrala
authors.forename: Anna
authors.initials: A
authors.lastname: Wolańczyk-Medrala
authors.forename: Irena
authors.initials: I
authors.lastname: Kustrzeba-Wójcicka
authors.forename: Jerzy
authors.initials: J
authors.lastname: Liebhart
authors.forename: Józef
authors.initials: J
authors.lastname: Małolepszy
mesh_terms: D000328:Adult; D000368:Aged; D000485:Allergens / Q000008:administration & dosage; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000209:etiology; D007159:Immunologic Tests / Q000379:methods*; D015289:Leukotrienes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015166:Monitoring, Immunologic / Q000379:methods; D010400:Penicillin G; D010406:Penicillins / Q000009:adverse effects*; D011107:Polylysine; D015203:Reproducibility of Results; D012882:Skin Tests
chemical_list: D000485:Allergens; D015289:Leukotrienes; D010406:Penicillins; D011107:Polylysine; D010400:Penicillin G
vernacular_title: Wartość testu wytwarzania leukotrienów w diagnostyce natychmiastowych reakcji alergicznych wywoływanych przez penicyline.