Elevated IL-1beta contributes to antibody suppression produced by stress.
Acute stressor exposure can facilitate innate immunity and suppress acquired immunity. The present study further characterized the potentiating effect of stress on innate immunity, interleukin-1beta (IL-1beta), and demonstrated that stress-induced potentiation of innate immunity may contribute to the stress-induced suppression of acquired immunity. The long-term effect of stress on IL-1beta was measured by using an ex vivo approach. Sprague-Dawley rats were challenged with lipopolysaccharide (LPS) in vivo, and the IL-1beta response was measured in vitro. Splenocytes, mesenteric lymphocytes, and peritoneal cavity cells had a dose- and time-dependent ex vivo IL-1beta response to LPS. Rats that were exposed to inescapable shock (IS, 100 1.6 mA, 5-s tail shocks, 60-s intertrial interval) and challenged with a submaximal dose of LPS 4 days later had elevated IL-1beta measured ex vivo. To test whether the acute stress-induced elevation in IL-1beta contributes to the long-term suppression in acquired immunity, IL-1beta receptors were blocked for 24 h after stress. Serum anti-keyhole limpet hemocyanin (KLH) immunoglobulin (Ig) was measured. In addition, the acute elevation (2 h post-IS) of splenic IL-1beta in the absence of antigen was verified. Interleukin-1 receptor antagonist prevented IS-induced suppression in anti-KLH Ig. These data support the hypothesis that stress-induced increases in innate immunity (i.e., IL-1beta) may contribute to stress-induced suppression in acquired immunity (i.e., anti-KLH Ig).
Moraska
Albert
A
Department of Kinesiology and Applied Physiology, University of Colorado at Boulder, 80309, USA.
Campisi
Jay
J
Nguyen
Kien T
KT
Maier
Steven F
SF
Watkins
Linda R
LR
Fleshner
Monika
M
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004597:Electroshock; D004797:Enzyme-Linked Immunosorbent Assay; D006433:Hemocyanins / Q000276:immunology; D007111:Immunity, Cellular / Q000187:drug effects; D007074:Immunoglobulin G / Q000096:biosynthesis; D007375:Interleukin-1 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000276:immunology*
D007074:Immunoglobulin G; D007375:Interleukin-1; D008070:Lipopolysaccharides; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin