Isolation of genes coding for Ade2 and Ura3 homologues from the multinuclear yeast Dipodascus magnusii.
Dipodascus magnusii is a nonconventional yeast species with giant multinuclear cells. We constructed two genomic DNA libraries in plasmid vectors and isolated the first two D. magnusii protein-encoding genes, DmADE2 and DmURA3, coding for phosphoribosylaminoimidazole carboxylase and orotidine-5'-phosphate decarboxylase, respectively. Both genes represent functional orthologues, since they complement ade2 and ura3 mutations in Saccharomyces cerevisiae and their putative products possess conserved sequences important for enzymatic activities. Moreover, the results of Southern blot analysis indicate that the genome of D. magnusii contains additional, paralogous sequences of the DmADE2 and DmURA3 genes.
Kucej
Martin
M
Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-1, 84215 Bratislava, Slovak Republic. kucej@fns.uniba.sk
Kosa
Peter
P
Adamíková
Lubica
L
Nosek
Jozef
J
D015139:Blotting, Southern; D002262:Carboxy-Lyases / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005656:Fungal Proteins / Q000235:genetics*; D015723:Gene Library; D005810:Multigene Family; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics*; D017422:Sequence Analysis, DNA
D005656:Fungal Proteins; D002262:Carboxy-Lyases; C022378:phosphoribosylaminoimidazole carboxylase; D009964:Orotidine-5'-Phosphate Decarboxylase