title: The impact of in vivo Calmette-Guérin Bacillus administration on in vitro IgE secretion in atopic children.
abstract: To investigate whether a preexisting T helper (T(H)) 2 type immune response could be suppressed by Calmette-Guérin Bacillus (BCG) immunization in atopic children with asthma, we determined interferon (IFN)-gamma, interleukin (IL)-2, IL-4, and IL-5 and total IgE level in the supernatant of peripheral blood mononuclear cells (PBMC) of six atopic and five nonatopic children in response to phytohemagglutinin A (PHA), purified protein derivate (PPD), and Dermatophagoides pteronyssinus II allergen (Der p II) both before and after BCG vaccination. IL-5 level in response to Der p II was significantly higher in the atopic group than in the nonatopic group both before and after BCG vaccination (p = 0.004, p = 0.009, respectively). In the atopic group, IgE levels determined in PPD and Der p II stimulated and unstimulated culture supernatants decreased significantly after BCG vaccination (p = 0.028, p = 0.026, p = 0.046, respectively), whereas in the nonatopic group (p = 0.041) BCG vaccination resulted in a significant decrease in IgE level only in response to Der p II stimulation. We concluded that in vivo BCG administration can downregulate both spontaneous and stimulated in vitro IgE secretion from PBMC of atopic children.
authors.affiliation: Marmara University Hospital, Department of Pediatrics, Istanbul, Turkey. aonder@attglobal.net
authors.forename: Işil B
authors.initials: IB
authors.lastname: Barlan
authors.forename: Faruk
authors.initials: F
authors.lastname: Tükenmez
authors.forename: Nerin N
authors.initials: NN
authors.lastname: Bahçeciler
authors.forename: Müjdat M
authors.initials: MM
authors.lastname: Başaran
mesh_terms: D001249:Asthma / Q000276:immunology*; D001500:BCG Vaccine / Q000276:immunology*; D002648:Child; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis*
chemical_list: D001500:BCG Vaccine; D007073:Immunoglobulin E