Effects of adsorption of acellular pertussis antigens onto different aluminium salts on the protective activity in an intranasal murine model of Bordetella pertussis infection.
Adsorption of the pertussis antigens, pertussis toxoid (PT), filamentous hemagglutinin (FHA) and pertactin (PRN) onto aluminium phosphate rather than aluminium hydroxide leads to a lower humoral response and poorer protection against intranasal pertussis challenge in mice. These effects could be reversed by inclusion of fimbriae (FIM) 2 and 3 in the formulation. These data emphasis the importance of correct formulation for such vaccines.
Denoël
P
P
Research and Development, GlaxoSmithKline Biologicals, Rue de l'Institut 89, B-1330 Rixensart, Belgium.
Poolman
J
J
Carletti
G
G
Veitch
K
K
D000281:Administration, Intranasal; D000327:Adsorption; D000535:Aluminum / Q000737:chemistry*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000737:chemistry*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D005260:Female; D006388:Hemagglutinins / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D010567:Pertussis Vaccine / Q000008:administration & dosage* / Q000276:immunology; D014121:Toxoids / Q000737:chemistry*; D010566:Virulence Factors, Bordetella / Q000737:chemistry*; D014917:Whooping Cough / Q000276:immunology / Q000517:prevention & control*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D006388:Hemagglutinins; D010567:Pertussis Vaccine; D014121:Toxoids; D010566:Virulence Factors, Bordetella; C051093:pertussis toxoid; C068929:pertactin; D000535:Aluminum