Design and synthesis of biotin chain-terminated glycopolymers for surface glycoengineering.
Biotin chain-terminated glycopolymers were generated by cyanoxyl-mediated free-radical polymerization using a biotin-derivatized arylamine initiator with high conversion (75%) and low polydispersity (1.30). Streptavidin-biotinylated glycopolymer binding was verified by SDS-PAGE gel shift assay and patterned glycocalyx-mimetic surfaces successfully fabricated.
Sun
Xue-Long
XL
Laboratory for Biomolecular Materials Research, Department of Surgery, Emory University School of Medicine, Atlanta, Georgia 30322, USA. xsun@emory.edu
Faucher
Keith M
KM
Houston
Michelle
M
Grande
Daniel
D
Chaikof
Elliot L
EL
D001704:Biopolymers / Q000737:chemistry*; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D006027:Glycosides / Q000138:chemical synthesis* / Q000737:chemistry; D007785:Lactose / Q000737:chemistry; D037102:Lectins / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D019809:Streptavidin / Q000737:chemistry
D001704:Biopolymers; D006027:Glycosides; D037102:Lectins; D001710:Biotin; D019809:Streptavidin; D007785:Lactose