title: Analysis of minicircle classes in Leishmania (Viannia) species.
abstract: This paper reports the analysis of minicircle sequence classes from 4 Leishmania species, all belonging to the 'New World' species of the subgenus Viannia: Leishmania braziliensis, L. guyanensis, L. panamensis and L. peruviana. A minicircle library was constructed for each species, and clones were analysed by restriction enzyme digest and sequence analysis. 319 minicircles from the 4 species were examined and 96 of these were wholly or partially sequenced. The sequences of 41 whole minicircles--21 from L. panamensis, 8 from L. guyanensis and 6 each from L. braziliensis and L. peruviana are presented. Sequence classes were identified within which sequences were highly conserved, with only a small number of single base pair changes between them. In contrast, minicircles from different classes differed significantly, displaying sequence homology only over the minicircle conserved region. Some minicircle classes were identified which were shared between species. These minicircles displayed sequence variation which was potentially species-specific, and were analysed phylogenetically. These results question the hypothesis that minicircle sequence is rapidly evolving and also suggest that an as yet unknown selective pressure maintains sequence class conservation over the entire minicircle molecule even in different species, not only over the conserved region and the guide ribonucleic acid gene. A novel hypothesis is proposed to explain these results.
authors.affiliation: Molteno Laboratories, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
authors.forename: S
authors.initials: S
authors.lastname: Brewster
authors.forename: D C
authors.initials: DC
authors.lastname: Barker
mesh_terms: D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D018105:DNA, Kinetoplast / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D007891:Leishmania / Q000235:genetics*; D007892:Leishmania braziliensis / Q000235:genetics; D018334:Leishmania guyanensis / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013045:Species Specificity
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan