In vitro and in vivo degradation studies for development of a biodegradable patch based on poly(3-hydroxybutyrate).
For the development of a resorbable gastrointestinal patch, the in vitro degradation of solution-cast films of poly(3-hydroxybutyrate) (PHB), modifications of PHB expected to influence its degradation time, as well a poly(L-lactide) (PLLA) was examined. The molecular weight of pure PHB decreased by one-half after 1 year in buffer solution (pH 7.4, 37 degrees C). Acceleration in molecular weight decrease was observed by blending with atactic PHB, whereas no influence was found with low-molecular weight PHB. Leaching of a water-soluble additive led to a slight acceleration of PHB degradability. In contrast, a deceleration in degradation rate was observed with the addition of a hydrophobic plasticizer. In vitro tests indicated an accelerating effect of pancreatin on PHB degradation, whereas PLLA degradation remained essentially uninfluenced. In comparison to simple hydrolysis, the degradation rate of PHB was accelerated about threefold. From the in vitro results, a PHB/atactic PHB blend was selected for repair of a bowel defect in Wistar rats. A patch film was fabricated by a dipping/leaching method. Twenty-six weeks post-implantation, material remnants were found in only one of four animals. The bowel defects were closed in all cases. It could be assessed that the patch material resists the intestinal secretions for a sufficiently long time but that it finally degrades completely.
Freier
Thomas
T
Institute for Biomedical Engineering, University of Rostock, Germany. thomas.freier@chemie.uni-rostock.de
Kunze
Carmen
C
Nischan
Claudia
C
Kramer
Sven
S
Sternberg
Katrin
K
Sass
Marko
M
Hopt
Ullrich T
UT
Schmitz
Klaus-Peter
KP
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001673:Biodegradation, Environmental; D002384:Catalysis; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000737:chemistry* / Q000494:pharmacology; D007422:Intestines / Q000187:drug effects; D010969:Plastics / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry; D000091402:Prohibitins; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature; D013997:Time Factors; D000094122:Polyhydroxybutyrates
D001672:Biocompatible Materials; D006885:Hydroxybutyrates; D010969:Plastics; D011091:Polyesters; D011108:Polymers; D000091402:Prohibitins; C000717332:Phb protein, rat; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C033616:poly(lactide)