Cathepsin D is specifically inhibited by deoxyribonucleic acids.
A cathepsin D (CD) inhibitor was searched using mouse embryonic fibroblasts deficient for CD. Synthetic DNA fragments specifically inhibited CD activity in a dose-dependent manner, but not the activities of other serine or cysteine proteinases. Cathepsin E activity was also inhibited by DNA fragments when hemoglobin was used as a substrate. CD inhibition by DNA fragments appeared to be electrostatic in nature and dependent on Tm values. Moreover, CD activity was partly inhibited by exogenously ingested DNA fragments, suggesting that DNA fragments with high Tm values are potent inhibitors of CD in vitro and partly in vivo.
Shibata
Masahiro
M
Department of Cell Biology and Neuroscience, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan.
Koike
Masato
M
Waguri
Satoshi
S
Zhang
Guiqin
G
Koga
Tomoyuki
T
Uchiyama
Yasuo
Y
D000818:Animals; D002402:Cathepsin D / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004247:DNA / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004705:Endocytosis / Q000502:physiology; D005347:Fibroblasts / Q000201:enzymology; D006454:Hemoglobins / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D051379:Mice
D006454:Hemoglobins; D004247:DNA; D002402:Cathepsin D