Characterization of a monoclonal antibody against desmoplakin 1 for use in dogs.
Skin biopsy specimens from face, axilla, abdomen and thigh, mucocutaneous tissues from anus and vagina, and oral mucosa from six healthy Beagle dogs were examined for desmoplakin (Dsp) immunoreactivity using immunoblotting and immunohistochemical analysis. With immunoblotting using mouse antihuman Dsp 1 monoclonal antibody (DP2.17), a band was detected at 250 kDa in all the extracts as in normal human skin samples, although no band was detected at 210 kDa, suggesting that monoclonal antibody DP2.17 recognizes canine Dsp 1 but not Dsp 2. Moreover, the desmosome regions of all specimens were stained with DP2.17 using immunohistochemical analysis. From these results, DP2.17, developed for the examination of human skin, might be suitable for the investigation of Dsp-related skin disorders in dogs.
Nakamura
Yuka
Y
Department of Veterinary Internal Medicine, Nihon University, School of Veterinary Medicine, Kanagawa, Japan. yuka@brs.nihon-u.ac.jp
Nakashima
Ko
K
Kano
Rui
R
Watanabe
Shinichi
S
Hasegawa
Atsuhiko
A
D000818:Animals; D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins / Q000276:immunology*; D051180:Desmoplakins; D003896:Desmosomes / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D006801:Humans; D015151:Immunoblotting / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D010392:Pemphigus / Q000175:diagnosis / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins; C495294:DSP protein, human; D051180:Desmoplakins