title: Cardiac gene expression profile and lipid accumulation in response to starvation.
abstract: Starvation induces many biochemical and histological changes in the heart; however, the molecular events underlying these changes have not been fully elucidated. To explore the molecular response of the heart to starvation, microarray analysis was performed together with biochemical and histological investigations. Serum free fatty acids increased twofold in both 16- and 48-h-fasted mice, and cardiac triglyceride content increased threefold and sixfold in 16- and 48-h-fasted mice, respectively. Electron microscopy showed numerous lipid droplets in hearts of 48-h-fasted mice, whereas fewer numbers of droplets were seen in hearts from 16-h-fasted mice. Expression of 11,000 cardiac genes was screened by microarrays. More than 50 and 150 known genes were detected by differential expression analysis after 16- and 48-h-fasts, respectively. Genes for fatty acid oxidation and gluconeogenesis were increased, and genes for glycolysis were decreased. Many other genes for metabolism, signaling/cell cycle, cytoskeleton, and tissue antigens were affected by fasting. These data provide a broad perspective of the molecular events occurring physiologically in the heart in response to starvation.
authors.affiliation: Third Department of Internal Medicine, Fukui Medical University, Fukui 910-1193, Japan. jinya@fmsrsa.fukui-med.ac.jp
authors.forename: Jinya
authors.initials: J
authors.lastname: Suzuki
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Shen
authors.forename: Brett D
authors.initials: BD
authors.lastname: Nelson
authors.forename: Simon P
authors.initials: SP
authors.lastname: Selwood
authors.forename: Greer M
authors.initials: GM
authors.lastname: Murphy
authors.forename: Hideo
authors.initials: H
authors.lastname: Kanehara
authors.forename: Sadao
authors.initials: S
authors.lastname: Takahashi
authors.forename: Koji
authors.initials: K
authors.lastname: Oida
authors.forename: Isamu
authors.initials: I
authors.lastname: Miyamori
authors.forename: Fredric B
authors.initials: FB
authors.lastname: Kraemer
authors.forename: Hideo
authors.initials: H
authors.lastname: Kanefara
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001835:Body Weight / Q000502:physiology; D002453:Cell Cycle / Q000235:genetics; D003599:Cytoskeleton / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005943:Gluconeogenesis / Q000235:genetics; D006019:Glycolysis / Q000235:genetics; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction; D015398:Signal Transduction / Q000235:genetics; D013217:Starvation / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D008055:Lipids