Latent TGF-beta binding protein-3 (LTBP-3) requires binding to TGF-beta for secretion.
Latent transforming growth factor-beta (TGF-beta) binding protein (LTBP)-1, which is easily secreted, has been shown to enhance the secretion of TGF-beta. Here we show that another member of the LTBP family, LTBP-3, is not secreted by several cell types, but secretion occurs after coexpression with TGF-beta. The secretion of LTBP-3 requires complexing of LTBP-3 with Cys33 of the TGF-beta propeptide.
Chen
Yan
Y
Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA.
Dabovic
Branka
B
Annes
Justin P
JP
Rifkin
Daniel B
DB
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D003545:Cysteine / Q000378:metabolism*; D004220:Disulfides / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D053779:Latent TGF-beta Binding Proteins; D051379:Mice; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D004220:Disulfides; D047908:Intracellular Signaling Peptides and Proteins; C509032:LTBP1 protein, human; C509038:LTBP3 protein, human; D053779:Latent TGF-beta Binding Proteins; C509033:Ltbp1 protein, mouse; C509039:Ltbp3 protein, mouse; D011993:Recombinant Fusion Proteins; D016212:Transforming Growth Factor beta; D003545:Cysteine