title: IFNgamma and TNFalpha account for a pro-clonogenic activity secreted by activated murine peritoneal macrophages.
abstract: In the present study, we found that murine peritoneal macrophages elicited by BCG or Listeria monocytogenes release into the media an activity capable of stimulating the lung colonization as well as the expression of MHC class I antigens in B16 melanoma cells. A similar activity has previously been found in media conditioned by Corynebacterium parvum-elicited macrophages. Analysis by gel filtration chromatography of media conditioned by Corynebacterium parvum-, BCG- or Listeria monocytogenes-elicited macrophages revealed that the material responsible for the pro-clonogenic activity concentrated in chromatographic fractions corresponding to molecular weights (25 to 52 kDa) which are characteristic of certain cytokines. Thus, we challenged the various macrophage-conditioned media with polyclonal antibodies against IFNgamma and TNFalpha, and found that the macrophage pro-clonogenic activity was completely abolished in the presence of anti-IFNgamma antibodies, but only partially inhibited by anti-TNFalpha antibodies. This finding suggests a cooperative participation of the two cytokines to the pro-clonogenic activity of the media conditioned by Corynebacterium parvum-, BCG- or Listeria monocytogenes-elicited macrophages.
authors.affiliation: Department of Experimental Pathology and Oncology, University of Florence, Italy. lcalorini@unifi.it
authors.forename: Lido
authors.initials: L
authors.lastname: Calorini
authors.forename: Francesca
authors.initials: F
authors.lastname: Bianchini
authors.forename: Antonella
authors.initials: A
authors.lastname: Mannini
authors.forename: Gabriele
authors.initials: G
authors.lastname: Mugnai
authors.forename: Manuela
authors.initials: M
authors.lastname: Balzi
authors.forename: Aldo
authors.initials: A
authors.lastname: Becciolini
authors.forename: Salvatore
authors.initials: S
authors.lastname: Ruggieri
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000378:metabolism; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008175:Lung Neoplasms / Q000382:microbiology / Q000473:pathology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D010537:Peritoneum / Q000166:cytology*; D011425:Propionibacterium acnes / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma