Synthesis and biological evaluation of analogues of the peptaibol ampullosporin A.
A series of analogues of the fungal peptaibol type metabolite ampullosporin A containing modifications in the C and N terminus as well as alpha-aminoisobutyric acid (Aib) substitutions in different positions of the peptide were synthesized by solid phase synthesis using the 9-fluorenylmethyloxycarbonyl strategy. Depending on the sequence position, couplings were performed with 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate/1-hydroxybenzotriazole and tetramethylfluoroformamidinium hexafluorophosphate, respectively. The structures of the target peptides were analyzed by electrospray ionization mass spectrometry and chromatographic methods (high-performance liquid chromatography, thin-layer chromatography). The biological activities of these compounds have been evaluated by assaying their potencies for the induction of pigment formation on the fungus Phoma destructiva as well as for the induction of hypothermia and inhibition of locomotoric activity in mice and were compared to the naturally occurring ampullosporins. Native ampullosporin A and analogues with C-terminal Leu or Leu-NH(2) showed comparable activity in the pigmentation assay. Similarly, the ampullosporin A analogues with N-terminal aromatic amino acid residues, such as D-Trp and Tic, also have high potency for pigment formation. The peptides containing structural modifications of ampullosporin A by systematic replacement of Aib by Ala (Ala scan) displayed moderate or high activity in the pigmentation assay, whereas simultaneous substitution of all Aib residues by Ala and Ile, respectively, or by insertion of nonaromatic residues into position 1 resulted in a loss of the effect on P. destructiva. Most of the compounds with no or weak activity in the microbial assay were not active in the hypothermic test, too, except the compound with 1-amino-1-cyclohexane carboxylic acid in position 4 instead of Aib. However, only a few compounds with high potency for pigmentation induction were found to produce strong hypothermia in mice. Thus, in contrast to the native ampullosporins, we succeeded to a certain degree in differentiation of the bioactivities with our synthetic analogues.
Nguyen
Hoai-Huong
HH
Institut für Biochemie und Biophysik, Friedrich-Schiller-Universität Jena, Philosophenweg 12, D-07743 Jena, Germany.
Imhof
Diana
D
Kronen
Matthias
M
Schlegel
Brigitte
B
Härtl
Albert
A
Gräfe
Udo
U
Gera
Lajos
L
Reissmann
Siegmund
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D014150:Antipsychotic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D007035:Hypothermia / Q000139:chemically induced; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D054848:Peptaibols; D010455:Peptides; D010860:Pigments, Biological / Q000096:biosynthesis; D012018:Reflex / Q000187:drug effects; D021241:Spectrometry, Mass, Electrospray Ionization
D000900:Anti-Bacterial Agents; D014150:Antipsychotic Agents; D054848:Peptaibols; D010455:Peptides; D010860:Pigments, Biological; C109004:ampullosporin