Ubiquinol and a coenzyme Q reducing system protect platelet mitochondrial function of transfusional buffy coats from oxidative stress.
The conditions under which Coenzyme Q (CoQ) may protect platelet mitochondrial function of transfusional buffy coats from aging and from induced oxidative stress were investigated. The Pasteur effect, i.e. the enhancement of lactate production after inhibition of mitochondrial respiratory chain, was exploited as a marker of mitochondrial function as it allows to calculate the ratio of mitochondrial ATP to glycolytic ATP. Reduced CoQ10 improves platelet mitochondrial function of transfusional buffy coats and protects the cells from induced oxidative stress. Oxidized CoQ is usually less effective, despite the presence, shown for the first time in this study, of quinone reductase activities in the platelet plasma membranes. The addition of a CoQ reducing system to platelets is effective in enhancing the protection of platelet mitochondrial function from the oxidative stress. The results support on one hand a possibility of protection of mitochondrial function in aging by exogenous CoQ intake, on the other a possible application in protection of transfusional buffy coats from storage conditions and oxidative deterioration.
Merlo Pich
Milena
M
Dipartimento di Biochimica G. Moruzzi, Università di Bologna, Italy.
Castagnoli
Alida
A
Biondi
Annalisa
A
Bernacchia
Andrea
A
Tazzari
Pier Luigi
PL
D'Aurelio
Marilena
M
Parenti Castelli
Giovanna
G
Formiggini
Gabriella
G
Conte
Roberto
R
Bovina
Carla
C
Lenaz
Giorgio
G
D000375:Aging / Q000502:physiology*; D000975:Antioxidants / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism; D019069:Cell Respiration / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D003067:Coenzymes; D019610:Cytoprotection; D004579:Electron Transport; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D015227:Lipid Peroxidation; D008928:Mitochondria / Q000502:physiology*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010100:Oxygen / Q000378:metabolism; D017713:Platelet Transfusion; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000975:Antioxidants; D003067:Coenzymes; D017392:Thiobarbituric Acid Reactive Substances; D014451:Ubiquinone; D019344:Lactic Acid; D016660:NAD(P)H Dehydrogenase (Quinone); C024989:coenzyme Q10; C003741:ubiquinol; D010100:Oxygen