Immunoaffinity-isolated antigens induce protective immunity against larval Strongyloides stercoralis in mice.
The objective of this study was to identify soluble protein antigens that would induce protective immunity against infective-stage larvae (L-3) of Strongyloides stercoralis in mice. Deoxycholate (DOC)-soluble proteins derived from L-3, adsorbed to aluminum hydroxide, induced protective immunity in BALB/c mice. The immunized mice generated parasite-specific IgG that could transfer passive immunity to naïve animals. The protective antibodies bound to parasite antigens found in the muscles and nerve cords of the L-3. An IgG affinity chromatography column generated with IgG from the sera of DOC-immunized mice was used to purify specific larval antigens. Proteins were eluted from the affinity column with sizes of 80, 75, 61, 57, 43, and 32 kDa. This antigen pool stimulated both proliferation and IL-5 production by splenocytes recovered from mice immunized with live L-3. Vaccination of mice with the immunoaffinity-isolated antigens led to significant protective immunity, with 83% of challenge larvae killed. This study demonstrates that IgG-isolated proteins are candidate antigens for a vaccine against larval S. stercoralis.
Herbert
De'Broski R
DR
Department of Microbiology and Immunology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA.
Nolan
Thomas J
TJ
Schad
Gerhard A
GA
Lustigman
Sara
S
Abraham
David
D
D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003840:Deoxycholic Acid / Q000737:chemistry; D015801:Helminth Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D007106:Immune Sera / Q000276:immunology; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000276:immunology; D015848:Interleukin-5 / Q000096:biosynthesis; D007814:Larva / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron; D012995:Solubility; D017171:Strongyloides stercoralis / Q000276:immunology*; D013322:Strongyloidiasis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000947:Antigens, Helminth; D015801:Helminth Proteins; D007106:Immune Sera; D007074:Immunoglobulin G; D015848:Interleukin-5; D003840:Deoxycholic Acid