title: Inhibitory effect of murine kidney extracts on mast cells derived from human umbilical cord blood.
abstract: We examined the effect of aqueous murine kidney extract (MKE) on the growth of mast cells prepared from the liquid culture of human umbilical cord blood CD34+ cells in the presence of a combination of recombinant human stem cell factor (SCF) and interleukin-6 (IL-6). Cultured cells were mature mast cells that expressed CD117 antigen on their surface, a specific marker for human mast cell, and they contained 6.53 pg histamine per cell. Adding MKE resulted in a 53% inhibition of mast cell growth and a 40% decrease in histamine content in mast cells in a serum-free liquid culture stimulated by SCF and IL-6. The inhibitory molecule for the growth of human mature mast cells was estimated at about 30 kDa of protein from gel-filtration HPLC. This fraction also inhibited the growth of murine peritoneal cells-derived mast cells. These results suggest that MKE contains regulator(s) that suppress the growth of mast cells and histamine synthesis. and that act beyond species specificity.
authors.affiliation: Laboratory of Radiopharmaceutical Sciences, Hokkaido College of Pharmacy, Otaru, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Murakami
authors.forename: Ikuo
authors.initials: I
authors.lastname: Kashiwakura
authors.forename: Yukitoshi
authors.initials: Y
authors.lastname: Hayase
authors.forename: Tsuneo A
authors.initials: TA
authors.lastname: Takahashi
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Takagi
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D005312:Fetal Blood / Q000187:drug effects*; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000737:chemistry*; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D051379:Mice; D011247:Pregnancy; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D018952:Antigens, CD34; D006131:Growth Inhibitors; D014020:Tissue Extracts