The occurrence of pathogenic Listeria monocytogenes and antibodies against listeriolysin-O in buffaloes.
The occurrence of Listeria monocytogenes in meat and milk samples, and antilisteriolysin O (ALLO) antibodies in sera of buffaloes were studied. Isolation of the pathogen was attempted from the samples by selective enrichment in University of Vermont Medium and plating onto Dominguez-Rodriguez isolation agar. The pathogenicity of the isolates was tested by Christie, Atkins, Munch Petersen test and mouse inoculation test. Of 167 meat samples 2.4 and 10.17% were positive for L. monocytogenes and Listeria sp., respectively. Of the 64 milk samples 6.25 and 26.13% were positive for L. monocytogenes and Listeria sp., respectively. A total of 284 serum samples were tested by listeriolysin O (LLO)-based indirect enzyme-linked immunosorbent assay of which 25.35% were found to be seropositive. The culture positivity for L. monocytogenes and detection of ALLO did not show any agreement (kappa = 0.035). The prevalence of pathogenic L. monocytogenes in milk and meat and the occurrence of anti-LLO antibodies is of concern from the public health point of view.
Barbuddhe
S B
SB
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar. barbuddhesb@yahoo.com
Chaudhari
S P
SP
Malik
S V S
SV
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001427:Bacterial Toxins; D002020:Buffaloes; D003257:Consumer Product Safety; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005516:Food Microbiology; D006360:Heat-Shock Proteins / Q000276:immunology*; D006460:Hemolysin Proteins; D006801:Humans; D007194:India / Q000453:epidemiology; D008089:Listeria monocytogenes / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D008088:Listeriosis / Q000453:epidemiology / Q000662:veterinary*; D008460:Meat / Q000382:microbiology; D008892:Milk / Q000382:microbiology; D015995:Prevalence; D011634:Public Health
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; C038705:hlyA protein, Listeria monocytogenes