title: Complete mapping of a novel HLA A*6801-restricted HIV-1 Tat epitope directly with a rapid modified enzyme-linked immunospot assay.
abstract: We identified a novel HLA A*6801-restricted HIV-1 Tat-derived cytotoxic T lymphocyte (CTL) epitope using an adapted enzyme-linked immunospot assay that allows the rapid ex vivo identification of CTL epitopes together with their associated HLA Class I restriction elements. The optimal 11 amino acid residue Tat epitope efficiently stabilized the refolding of monomeric peptide-HLA A6801 complexes in vitro and fluorochrome-labelled, tetrameric peptide-HLA A6801 complexes stained CD8 T cells specific for this epitope directly ex vivo.
authors.affiliation: Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford, UK.
authors.forename: Annette
authors.initials: A
authors.lastname: Oxenius
authors.forename: Bent K
authors.initials: BK
authors.lastname: Jakobsen
authors.forename: Philippa J
authors.initials: PJ
authors.lastname: Easterbrook
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Boulter
authors.forename: Tin
authors.initials: T
authors.lastname: Tun
authors.forename: Anele
authors.initials: A
authors.lastname: Waters
authors.forename: Juliet
authors.initials: J
authors.lastname: Agudelo
authors.forename: Martin
authors.initials: M
authors.lastname: Barnardo
authors.forename: Rodney E
authors.initials: RE
authors.lastname: Phillips
authors.forename: David A
authors.initials: DA
authors.lastname: Price
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping / Q000379:methods*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015696:Gene Products, tat / Q000737:chemistry / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology*; D006801:Humans; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D018984:Epitopes, T-Lymphocyte; D015696:Gene Products, tat; D015234:HLA-A Antigens; D054322:tat Gene Products, Human Immunodeficiency Virus