title: Effect of monensin on the levels of tachykinins and their processing enzyme activity in rat dorsal root ganglia.
abstract: Th effect of monensin, which inhibits trans-Golgi function, on the levels of tachykinins and their processing enzyme activity was examined in organ-cultured rat dorsal root ganglia (DRG). Using an enzyme immunoassay method, we measured neurokinin A and substance P immunoreactivity in the DRG cultured for 72 h with and without 0.1 microM monensin. Both tachykinins were reduced in the DRG treated with monensin. Treatment with monensin also reduced the activity of carboxypeptidase E, which is one of the proteolytic processing enzymes of neuropeptides. These data suggest that proteolytic processing enzymes may in part modulate the biological activity of neuropeptides within a trans-Golgi apparatus.
authors.affiliation: Department of Hygienic Chemistry, Showa Pharmaceutical University, Machida 194-8543, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Chikuma
authors.forename: Yuji
authors.initials: Y
authors.lastname: Inomata
authors.forename: Ken
authors.initials: K
authors.lastname: Tsuchida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hojo
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kato
mesh_terms: D000818:Animals; D043423:Carboxypeptidase H; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors*; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000378:metabolism*; D007476:Ionophores / Q000494:pharmacology*; D008297:Male; D008985:Monensin / Q000494:pharmacology*; D015288:Neurokinin A / Q000037:antagonists & inhibitors*; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D013373:Substance P / Q000037:antagonists & inhibitors*
chemical_list: D007476:Ionophores; D013373:Substance P; D015288:Neurokinin A; D008985:Monensin; D002268:Carboxypeptidases; D043423:Carboxypeptidase H