Recruitment of mammalian cell fibronectin to the surface of Chlamydia trachomatis.
Pathogenic bacteria exploit the presence of various host cell molecules in order to colonize new tissues. Fibronectin is involved in a wide range of cell functions in vivo, and staphylococci, streptococci, and gonococci have evolved mechanisms to utilize this glycoprotein to mediate host cell binding. We show that elementary bodies (EB) from two biovars of Chlamydia trachomatis recruit fibronectin to their surfaces upon lysis of the host cell. We also demonstrate that a heparan sulfate lyase-sensitive molecule on chlamydial EB is responsible for binding at least a portion of this fibronectin.
Kleba
Betsy J
BJ
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA. .
Banta
Erin
E
Lindquist
Erika A
EA
Stephens
Richard S
RS
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002692:Chlamydia trachomatis / Q000378:metabolism*; D005353:Fibronectins / Q000302:isolation & purification / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007739:L Cells; D008024:Ligands; D008322:Mammals; D051379:Mice; D011133:Polysaccharide-Lyases / Q000378:metabolism; D017469:Receptors, Fibronectin / Q000378:metabolism
D005353:Fibronectins; D008024:Ligands; D017469:Receptors, Fibronectin; D011133:Polysaccharide-Lyases; C021349:heparitinsulfate lyase