title: Effect of denture cleaner using ozone against methicillin-resistant Staphylococcus aureus and E. coli T1 phage.
abstract: We examined the bactericidal and virucidal effectiveness of a denture cleaner that uses ozone (ozone concentration, 10 ppm) against methicillin-resistant Staphylococcus aureus (MRSA) and T1 phage, respectively. In the bactericidal activity test, with the ozone supply turned on, the number of bacteria was 3.1 x 10(3) CFU/mL at the beginning of the experiment, fell to 1.0 x 10(0) CFU/mL 10 min later, and was 1.0 x 10(0) CFU/mL or less afterwards. In contrast, when the ozone supply was cut off (air bubble only), the number of bacteria was 3.4 x 10(3) CFU/mL at the beginning of the experiment, and had fallen to 3.0 x 10(3) CFU/mL 60 min later (no statistically significant difference). In the virucidal activity test, the number of phages was 1.2 x 10(6) PFU/mL before ozone treatment, fell to about 1/10 of that number 10 min later, and was 6.1 x 10(0) PFU/mL 40 min later. These results indicate that the use of ozone in this denture cleaner is effective against MRSA and viruses.
authors.affiliation: Department of Fixed Prosthodontics, Division of Biomaterials, Research Institute of Advanced Oral Science, School of Dentistry, Aichi-Gakuin University, 2-11 Suemori-dori, Chikusa-ku, Nagoya, Japan, 464-8651.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murakami
authors.forename: Miho
authors.initials: M
authors.lastname: Mizuguchi
authors.forename: Masami
authors.initials: M
authors.lastname: Hattori
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ito
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Kawai
authors.forename: Jiro
authors.initials: J
authors.lastname: Hasegawa
mesh_terms: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D003820:Denture Cleansers / Q000494:pharmacology*; D004867:Equipment Design; D006801:Humans; D016106:Methicillin Resistance; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D010126:Ozone / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013223:Statistics as Topic; D013604:T-Phages / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000998:Antiviral Agents; D003820:Denture Cleansers; D010083:Oxidants, Photochemical; D010126:Ozone