Immunoassay with a microtiter plate incorporated multichannel electrochemical detection system.
To extend the application of a multichannel electrochemical detector (MED) for immunoassay, a MED system consisting of 8 sets of Pt electrodes in an arrangement fitted with the dimensions of a row of microtiter wells in a microtiter plate and a microcomputer-assisted 16-channel potentiostat was constructed. With this developed MED system, electroactive enzymatic products produced in eight microtiter wells can be analyzed simultaneously with a developed amperometric procedure. To demonstrate the applicability of the MED system for immunoassay, an immunosystem containing rabbit-IgG and alkaline phosphatase-conjugated goat anti rabbit-IgG was studied. From the dynamic range of 10-1000 ng/mL (0.064-6.4 pM) and the detection limit of 1.0 ng/mL (6.4 pM) obtained, the developed MED shows a tremendous improvement in sensitivity, detection limit, and efficiency compared with that obtained from conventional electrochemical immunoassay.
Tang
Tzyh-Chyang
TC
Department of Pharmacy, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
Deng
Anping
A
Huang
Hsuan-Jung
HJ
D000818:Animals; D004563:Electrochemistry; D004566:Electrodes; D007118:Immunoassay / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000276:immunology; D007202:Indicators and Reagents; D010710:Phosphates / Q000737:chemistry; D011817:Rabbits
D007074:Immunoglobulin G; D007202:Indicators and Reagents; D010710:Phosphates