Characterization of a cell-associated inulosucrase from a novel source: a Leuconostoc citreum strain isolated from Pozol, a fermented corn beverage of Mayan origin.
A cell-associated fructosyltransferase was extracted from a novel source, a strain of Leuconostoc citreum isolated from Pozol, a Mexican traditional fermented corn beverage, where lactic microflora are partially responsible for the transformation process. The enzyme is associated with the cell wall. It was characterized both in its cell-associated insoluble form and after separation by urea treatment. The fructosyltransferase has a molecular mass of 170 kDa, the highest reported for this type of enzyme, and in its insoluble form is highly specific for polymer synthesis, with low fructose transferred to maltose and lactose added to the reaction medium (acceptor reactions). The synthesized polymer has an inulin-like structure with beta2-1 glycosidic linkages, as demonstrated by 13C nuclear magnetic resonance (NMR). Bacterial inulosucrases have only been reported in Streptococcus mutans.
Olivares-Illana
V
V
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
Wacher-Odarte
C
C
Le Borgne
S
S
López-Munguía
A
A
D001628:Beverages; D005285:Fermentation; D016695:Glycosyltransferases / Q000378:metabolism; D006602:Hexosyltransferases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007934:Leuconostoc / Q000201:enzymology*; D008970:Molecular Weight; D012995:Solubility; D003313:Zea mays / Q000382:microbiology*; D001426:Bacterial Proteins
D016695:Glycosyltransferases; D006602:Hexosyltransferases; C021451:inulosucrase; D001426:Bacterial Proteins