title: In vitro and in vivo degradation studies for development of a biodegradable patch based on poly(3-hydroxybutyrate).
abstract: For the development of a resorbable gastrointestinal patch, the in vitro degradation of solution-cast films of poly(3-hydroxybutyrate) (PHB), modifications of PHB expected to influence its degradation time, as well a poly(L-lactide) (PLLA) was examined. The molecular weight of pure PHB decreased by one-half after 1 year in buffer solution (pH 7.4, 37 degrees C). Acceleration in molecular weight decrease was observed by blending with atactic PHB, whereas no influence was found with low-molecular weight PHB. Leaching of a water-soluble additive led to a slight acceleration of PHB degradability. In contrast, a deceleration in degradation rate was observed with the addition of a hydrophobic plasticizer. In vitro tests indicated an accelerating effect of pancreatin on PHB degradation, whereas PLLA degradation remained essentially uninfluenced. In comparison to simple hydrolysis, the degradation rate of PHB was accelerated about threefold. From the in vitro results, a PHB/atactic PHB blend was selected for repair of a bowel defect in Wistar rats. A patch film was fabricated by a dipping/leaching method. Twenty-six weeks post-implantation, material remnants were found in only one of four animals. The bowel defects were closed in all cases. It could be assessed that the patch material resists the intestinal secretions for a sufficiently long time but that it finally degrades completely.
authors.affiliation: Institute for Biomedical Engineering, University of Rostock, Germany. thomas.freier@chemie.uni-rostock.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Freier
authors.forename: Carmen
authors.initials: C
authors.lastname: Kunze
authors.forename: Claudia
authors.initials: C
authors.lastname: Nischan
authors.forename: Sven
authors.initials: S
authors.lastname: Kramer
authors.forename: Katrin
authors.initials: K
authors.lastname: Sternberg
authors.forename: Marko
authors.initials: M
authors.lastname: Sass
authors.forename: Ullrich T
authors.initials: UT
authors.lastname: Hopt
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Schmitz
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001673:Biodegradation, Environmental; D002384:Catalysis; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000737:chemistry* / Q000494:pharmacology; D007422:Intestines / Q000187:drug effects; D010969:Plastics / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry; D000091402:Prohibitins; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature; D013997:Time Factors; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D006885:Hydroxybutyrates; D010969:Plastics; D011091:Polyesters; D011108:Polymers; D000091402:Prohibitins; C000717332:Phb protein, rat; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C033616:poly(lactide)