The Spot Fitting and Expression Quantification for the Nylon Filter cDNA Microarray.
High-density cDNA microarray is important in monitoring large scalegenomic expression profile. With radiolabeled cDNA sample, nylon filter cDNA microarrays have high sensitivity and wide linear range of hybridization signals, so it can be employed to detect many important low-abundance cDNAs. However, the nylon filter tends to be randomly contaminated, and the array spots are prone to dispersion and saturation, resulting in inaccurate expression value. Based on aphysical model, we address a novel treatment with accurate positioning, complement of saturation and correction of interference. Thus the accurate quantificati on of expression can be extracted with remarkably improved reproducibility.
Zhang
Wen
W
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China. dingdafu@server.shcnc.ac.cn
Ding
Da-Fu
DF