title: Interleukin-1beta expression in human gastric carcinoma with Epstein-Barr virus infection.
abstract: The KT tumor is a transplantable strain of a human Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC), established in severe combined immunodeficiency (SCID) mice, with which the cytokine expression of EBVaGC can be investigated without interference from the infiltrating lymphocytes. As a part of a high-density oligonucleotide array (GeneChip) analysis of EBVaGC, the interleukin-1beta (IL-1beta) gene was the only cytokine gene that showed markedly higher expression in the KT tumor cells than in two tumor strains of EBV-negative GC. The results were confirmed by Northern blotting, Western blotting, and enzyme-linked immunosorbent assay. Furthermore, we demonstrated a positive signal for IL-1beta mRNA in the carcinoma cells of a surgically resected EBVaGC, but not in EBV-negative GC, by in situ hybridization. In vitro, IL-1beta increased the cell growth of a GC cell line, TMK1. Thus, IL-1beta may act as an autocrine growth factor in EBVaGC.
authors.affiliation: Department of Pathology, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan. jmchong-tky@umin.ac.jp
authors.forename: Ja-Mun
authors.initials: JM
authors.lastname: Chong
authors.forename: Kazuya
authors.initials: K
authors.lastname: Sakuma
authors.forename: Makoto
authors.initials: M
authors.lastname: Sudo
authors.forename: Toshio
authors.initials: T
authors.lastname: Osawa
authors.forename: Etsuko
authors.initials: E
authors.lastname: Ohara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uozaki
authors.forename: Junji
authors.initials: J
authors.lastname: Shibahara
authors.forename: Kenji
authors.initials: K
authors.lastname: Kuroiwa
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Tominaga
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Hippo
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Aburatani
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Funata
authors.forename: Masashi
authors.initials: M
authors.lastname: Fukayama
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000821:virology; D000818:Animals; D020031:Epstein-Barr Virus Infections / Q000378:metabolism*; D004854:Herpesvirus 4, Human; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000821:virology; D014407:Tumor Cells, Cultured
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger