title: Aspects of the maintenance of the life cycle of Fasciola hepatica in Lymnaea columella in Minas Gerais, Brazil.
abstract: Fascioliasis is a parasitic disease of domestic ruminants that occurs worldwide. The lymnaeid intermediate hosts of Fasciola hepatica include Lymnaea columella, which is widely distributed in Brazil. A colony of L. columella from Belo Horizonte, MG, was reared in our laboratory to be used in studies of the F. hepatica life cycle, the intermediate host-parasite relationship and development of an anti-helminthic vaccine. In the first experiment 1,180 snails were exposed to miracidia of F. hepatica eggs removed from the biliary tracts of cattle from the State of Rio Grande do Sul. In the second and third experiments the snails were exposed to miracidia that had emerged from F. hepatica eggs from Uruguay, maintained in rabbits. The rates of infection in the first, second and third experiments were 0, 42.1 and 0% respectively. Over 15,806 metacercariae were obtained and stored at 4 degrees C. Four rabbits weighing 1.5 kg each were infected with 32-44 metacercariae and two with 200. Three rabbits begin to eliminate eggs of the parasite in the feces from 84 days after infection onwards. The biological cycle of F. hepatica in L. columella and the rabbit was completed within 124 days.
authors.affiliation: Laboratório de Malacologia, Centro de Pesquisas René Rachou-Fiocruz, Fiocruz, Belo Horizonte, MG, 30190-002, Brasil. souzacp@cpqrr.fiocruz.br
authors.forename: Cecília Pereira
authors.initials: CP
authors.lastname: de Souza
authors.forename: Kelly Grace
authors.initials: KG
authors.lastname: Magalhaes
authors.forename: Liana Konovaloff Jannotti
authors.initials: LK
authors.lastname: Passos
authors.forename: Glaucia Cristina
authors.initials: GC
authors.lastname: Pereira  dos Santos
authors.forename: Fabio
authors.initials: F
authors.lastname: Ribeiro
authors.forename: Naftale
authors.initials: N
authors.lastname: Katz
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D005210:Fasciola hepatica / Q000254:growth & development*; D006790:Host-Parasite Interactions; D008018:Life Cycle Stages / Q000502:physiology*; D008195:Lymnaea / Q000469:parasitology*; D011817:Rabbits; D014583:Uruguay