title: Enterococcus faecalis induces inflammatory bowel disease in interleukin-10 knockout mice.
abstract: Germ-free interleukin-10 knockout (IL-10 KO) mice developed inflammatory bowel disease (IBD) after they were colonized with a pure culture of Enterococcus faecalis. E. faecalis not only induced IBD (primarily in colon and rectum) but rectal dysplasia and adenocarcinoma was also found in the IL-10 KO mice. Conventional (complex-intestinal flora) IL-10 KO mice developed IBD within 10 to 15 weeks of age and showed more pathology in the cecum (typhlitis) than we observed with E. faecalis-induced IBD in gnotobiotic IL-10 KO mice. Conversely, neither germ-free IL-10 mice nor IL-10 KO mice colonized as adults, with a pure culture of Candida albicans, Escherichia coli, Lactobacillus casei, L. reuteri, L. acidophilus, a Bifidobacterium sp., Lactococcus lactis, or a Bacillus sp. developed IBD during the 25- to 30-week study. E. faecalis is a common intestinal microbe of man and animals that can trigger IBD, dysplasia, and carcinoma in a genetically susceptible murine host.
authors.affiliation: Department of Surgery, University of Wisconsin Medical School, Madison, Wisconsin, USA. balish@musc.edu
authors.forename: Edward
authors.initials: E
authors.lastname: Balish
authors.forename: Thomas
authors.initials: T
authors.lastname: Warner
mesh_terms: D000818:Animals; D001644:Bifidobacterium; D002176:Candida albicans; D002432:Cecum / Q000473:pathology; D003106:Colon / Q000473:pathology; D013293:Enterococcus faecalis; D004926:Escherichia coli; D005856:Germ-Free Life; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology* / Q000473:pathology; D015212:Inflammatory Bowel Diseases / Q000382:microbiology* / Q000473:pathology; D016753:Interleukin-10 / Q000235:genetics / Q000502:physiology*; D007778:Lactobacillus; D007780:Lacticaseibacillus casei; D051379:Mice; D018345:Mice, Knockout
chemical_list: D016753:Interleukin-10