Isolates of Microbotryum violaceum from North American host species are phylogenetically distinct from their European host-derived counterparts.
Microbotryum violaceum is a basidiomycete that infects the anthers of its Caryophyllaceae host species. Individual fungal isolates are host limited, though they are not morphologically distinct. This study used variable regions of the highly conserved gamma-tubulin, beta-tubulin, and ribosomal RNA-encoding genes to determine the relationships among M. violaceum fungal isolates from different host species and different geographical locations. Phylogenetic trees from intron nucleotide sequences in two protein-coding genes were compared to trees produced from internal transcribed spacers (ITS) of rDNA. Phylogenetic analyses suggested that there are two clades, one from North America and one from Europe. Isolates from both clades grouped according to host species, although in some analyses isolates from closely related host species were placed together. These results are consistent with the view that M. violaceum has experienced cospeciation with its hosts.
Freeman
Angela B
AB
Department of Biology, University of Louisville, Louisville, Belknap-LS139, Louisville, KY 40208, USA.
Duong
K Kellye
KK
Shi
Tie-Liu
TL
Hughes
Carolyn F
CF
Perlin
Michael H
MH
D001487:Basidiomycota / Q000235:genetics* / Q000254:growth & development; D002467:Cell Nucleus / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005060:Europe; D008969:Molecular Sequence Data; D009656:North America; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D029751:Silene / Q000145:classification / Q000235:genetics* / Q000382:microbiology; D014404:Tubulin / Q000235:genetics
D004271:DNA, Fungal; D018744:DNA, Plant; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S; D012338:RNA, Ribosomal, 23S; D012340:RNA, Ribosomal, 5.8S; D014404:Tubulin