Characterization of fowl adenovirus serotype-4 associated with hydropericardium syndrome in chicken.
The polypeptides of three fowl adenovirus-4 (FAV-4) field isolates of hydropericardium syndrome from various geographical areas of the country and the standard FAV-1 (CELO virus) were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and analysed by protein immunoblotting with polyclonal antibodies to FAV-4 and FAV-1. Protein profile analysis of FAV-4 isolates revealed similarity of all the eight polypeptides with molecular weight ranging from 20 to 107 kDa but differed from CELO, particularly in their 24.2 kDa protein. Subsequent immunoblotting showed relatedness of at least five protein fractions of FAV-4 to CELO virus.
Balamurugan
V
V
Molecular Virology Laboratory, Bangalore, India.
Kataria
J M
JM
Kataria
R S
RS
Verma
K C
KC
Nanthakumar
T
T
D000257:Adenoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D002645:Chickens / Q000821:virology*; D004591:Electrophoresis, Polyacrylamide Gel; D025681:Fowl adenovirus A / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity; D010490:Pericardial Effusion / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D011201:Poultry Diseases / Q000473:pathology / Q000821:virology*; D013577:Syndrome; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology; D014774:Virulence
D014764:Viral Proteins