title: Controlled perfusion decreases reperfusion injury after high-flow reperfusion.
abstract: BACKGROUND
  Some investigators have suggested that high pulmonary artery flow rates increase the risk of severe reperfusion injury after lung transplantation. We hypothesized that controlling the initial flow rate and pulmonary artery pressure would decrease the severity of lung dysfunction in the setting of high-flow reperfusion.
  
  
  METHODS
  Using our isolated, ventilated, blood-perfused rabbit lung model, all groups underwent lung harvest, 4-hour storage (4 degrees C), and blood reperfusion. We measured pulmonary artery pressure, peak inspiratory pressure, arterial oxygenation, and wet-to-dry weight ratio. Group 1 (control, n = 8) underwent reperfusion at 60 ml/min for 30 minutes. Group 2 (high flow, n = 8) underwent reperfusion at 120 ml/min for 30 minutes. Group 3 (controlled flow, n = 8) underwent initial reperfusion at 60 ml/min for 5 minutes, followed by reperfusion at 120 ml/min for 25 minutes.
  
  
  RESULTS
  Group 1 had significantly improved pulmonary artery pressure, peak inspiratory pressure, arterial oxygenation, and wet-to-dry weight ratio measurements compared with groups 2 and 3 after 30 minutes of reperfusion. However, Group 3 had improved pulmonary artery pressure, peak inspiratory pressure, arterial oxygenation, and wet-to-dry weight ratio measurements compared with Group 2.
  
  
  CONCLUSIONS
  High-flow reperfusion results in severe reperfusion injury after lung transplantation. Controlled reperfusion using a low initial flow rate decreases the severity of reperfusion injury associated with high-flow rates.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA. smf9e@virginia.edu
authors.forename: Steven M
authors.initials: SM
authors.lastname: Fiser
authors.forename: Irving L
authors.initials: IL
authors.lastname: Kron
authors.forename: Stewart M
authors.initials: SM
authors.lastname: Long
authors.forename: Aditya K
authors.initials: AK
authors.lastname: Kaza
authors.forename: John A
authors.initials: JA
authors.lastname: Kern
authors.forename: David C
authors.initials: DC
authors.lastname: Cassada
authors.forename: Victor E
authors.initials: VE
authors.lastname: Laubach
authors.forename: Curtis G
authors.initials: CG
authors.lastname: Tribble
mesh_terms: D000818:Animals; D001794:Blood Pressure; D004195:Disease Models, Animal; D016040:Lung Transplantation; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion / Q000379:methods*; D009195:Peroxidase / Q000032:analysis; D011817:Rabbits; D015427:Reperfusion Injury / Q000517:prevention & control
chemical_list: D009195:Peroxidase