[Selection of Penicillium funiculosum strains with high glucose oxidase activity].
Metabolic inhibitors, riboflavin, and end products of glucose oxidation were shown to hold much promise for the selection of Penicillium funiculosum mutant strains with a high glucose oxidase activity. The incidence of positive mutations was highest in clones resistant to sodium azide, riboflavin, and beta-D-glucono-delta-lactone. Enzyme activity in Penicillium funiculosum mutants was studied under conditions of submerged cultivation. The intensity of glucose oxidase synthesis in seven cultures was 24-56% higher than that in the parent strain of Penicillium funiculosum NMM95.132.
Mikhaĭlova
R V
RV
Institute of Microbiology, Academy of Sciences of Belarus, Minsk, 220141 Belarus.
Semashko
T V
TV
Lobanok
A G
AG
D004352:Drug Resistance, Microbial; D005949:Glucose Oxidase / Q000096:biosynthesis / Q000378:metabolism*; D009154:Mutation; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D012256:Riboflavin / Q000494:pharmacology; D019810:Sodium Azide / Q000494:pharmacology
D019810:Sodium Azide; D005949:Glucose Oxidase; D012256:Riboflavin
Otbor shtammov Penicillium funiculosum--vysokoaktivnykh produtsentov gliukosooksidazy.