A study of tissue interface membranes from revision accord knee arthroplasty: the role of T lymphocytes.
Despite four decades of advances in the design of orthopaedic devices aseptic loosening remains a major cause for the revision of total joint arthroplasty. This study used the techniques of immunohistochemistry and reverse transcription polymerase chain reaction to identify the inflammatory cell types, cytokines and chemokines within the interface tissue surrounding failed Accord Knee prostheses. Many T cells were identified within the tissue: however, the classical marker of activation, CD25 was expressed on very few cells. Molecular analysis failed to detect the synthesis of either Th1 or Th2 cytokines. These results suggest that the T cells are being actively recruited to the site of inflammation along the chemokine gradients but are not participating in a classical immune response.
Baldwin
L
L
UK Centre for Tissue Engineering, Department of Clinical Engineering, Faculty of Medicine, Royal Liverpool University Hospital. lisab@liv.ac.uk
Flanagan
B F
BF
McLaughlin
P J
PJ
Parkinson
R W
RW
Hunt
J A
JA
Williams
D F
DF
D000368:Aged; D019645:Arthroplasty, Replacement, Knee; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D019544:Equipment Failure Analysis; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000503:physiopathology*; D007720:Knee Prosthesis; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology / Q000502:physiology; D011475:Prosthesis Failure; D012086:Reoperation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D013997:Time Factors
D016207:Cytokines