Human anti-rhesus D IgG1 antibody produced in transgenic plants.
Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.
Bouquin
Thomas
T
Department of Plant Physiology, Molecular Biology Institute, Copenhagen University, Denmark.
Thomsen
Mads
M
Nielsen
Leif Kofoed
LK
Green
Trine Hefsgaard
TH
Mundy
John
J
Dziegiel
Morten Hanefeld
MH
D000372:Agglutination Tests; D017360:Arabidopsis / Q000235:genetics*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics* / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D016897:Respiratory Burst; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D007074:Immunoglobulin G; D011994:Recombinant Proteins; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen