Increase of brain oxidative stress in mild cognitive impairment: a possible predictor of Alzheimer disease.
BACKGROUND
The isoprostane 8,12-iso-iPF(2alpha)-VI, a specific marker of in vivo lipid peroxidation, is increased in Alzheimer disease (AD). The pathological changes associated with AD have a long silent phase before the appearance of clinical symptoms. Several studies have shown that AD is preceded by a prodromal phase characterized by mild cognitive impairment (MCI).


OBJECTIVE
To investigate levels of this biomarker in subjects with MCI.


METHODS
Using gas chromatography-mass spectrometry analysis, we measured 8,12-iso-iPF(2alpha)-VI levels in urine, plasma, and cerebrospinal fluid of patients with AD, subjects with MCI, and cognitively normal elderly subjects.


METHODS
Subjects attending the Memory Disorders Clinic.


RESULTS
We found significantly higher 8,12-iso-iPF(2alpha)-VI levels in cerebrospinal fluid, plasma, and urine of subjects with MCI compared with cognitively normal elderly subjects.


CONCLUSIONS
These results imply that individuals with MCI have increased brain oxidative damage before the onset of symptomatic dementia. Measurement of this isoprostane may identify a subgroup of patients with MCI with increased lipid peroxidation who are at increased risk to progress to symptomatic AD.
Praticò
Domenico
D
Center for Experimental Therapeutics, University of Pennsylvania, BRB II/III, Room 812, 421 Curie Blvd, Philadelphia, PA 19104. domenico@spirit.gcrc.upenn.edu
Clark
Christopher M
CM
Liun
Feyan
F
Rokach
Joshua
J
Lee
Virginia Y-M
VY
Trojanowski
John Q
JQ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000175:diagnosis / Q000378:metabolism* / Q000523:psychology; D000704:Analysis of Variance; D001921:Brain / Q000378:metabolism*; D003072:Cognition Disorders / Q000378:metabolism* / Q000523:psychology; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D011237:Predictive Value of Tests