Supported lipid bilayers as effective substrates for atomic force microscopy.
Supported lipid bilayers offer a diverse set of substrates for AFM investigations of both water-soluble samples and integral membrane proteins. Although their amphipathic nature is necessary for the latter specimens, it is their robustness and easily changed surface characteristics that make these surfaces particularly attractive for the former ones. When starting any AFM investigation of water-soluble biological complexes, it is probably best to try mica first, owing to its remarkable effectiveness with a wide range of samples. Yet, if mica should prove inadequate, supported lipid bilayers are, as demonstrated here, a sensible second choice.
Czajkowsky
Daniel M
DM
Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA.
Shao
Zhifeng
Z
D000538:Aluminum Silicates / Q000737:chemistry; D002772:Cholera Toxin / Q000737:chemistry / Q000378:metabolism; D022162:Cytoplasmic Vesicles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000378:metabolism / Q000648:ultrastructure
D000538:Aluminum Silicates; D008024:Ligands; D008051:Lipid Bilayers; D008563:Membrane Lipids; D011506:Proteins; D002772:Cholera Toxin; C011934:mica