title: The mechanical hierarchies of fibronectin observed with single-molecule AFM.
abstract: Mechanically induced conformational changes in proteins such as fibronectin are thought to regulate the assembly of the extracellular matrix and underlie its elasticity and extensibility. Fibronectin contains a region of tandem repeats of up to 15 type III domains that play critical roles in cell binding and self-assembly. Here, we use single-molecule force spectroscopy to examine the mechanical properties of fibronectin (FN) and its individual FNIII domains. We found that fibronectin is highly extensible due to the unfolding of its FNIII domains. We found that the native FNIII region displays strong mechanical unfolding hierarchies requiring 80 pN of force to unfold the weakest domain and 200 pN for the most stable domain. In an effort to determine the identity of the weakest/strongest domain, we engineered polyproteins composed of an individual domain and measured their mechanical stability by single-protein atomic force microscopy (AFM) techniques. In contrast to chemical and thermal measurements of stability, we found that the tenth FNIII domain is mechanically the weakest and that the first and second FNIII domains are the strongest. Moreover, we found that the first FNIII domain can acquire multiple, partially folded conformations, and that their incidence is modulated strongly by its neighbor FNIII domain. The mechanical hierarchies of fibronectin demonstrated here may be important for the activation of fibrillogenesis and matrix assembly.
authors.affiliation: Department of Physiology and Biophysics, Mayo Foundation, Rochester, MN 55905, USA. oberhauser.andres@mayo.edu
authors.forename: Andres F
authors.initials: AF
authors.lastname: Oberhauser
authors.forename: Carmelu
authors.initials: C
authors.lastname: Badilla-Fernandez
authors.forename: Mariano
authors.initials: M
authors.lastname: Carrion-Vazquez
authors.forename: Julio M
authors.initials: JM
authors.lastname: Fernandez
mesh_terms: D000595:Amino Acid Sequence; D001696:Biomechanical Phenomena; D064211:Connectin; D005353:Fibronectins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*; D006801:Humans; D007700:Kinetics; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics; D020815:Polyproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D011489:Protein Denaturation; D015202:Protein Engineering; D017510:Protein Folding; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D064211:Connectin; D005353:Fibronectins; D009124:Muscle Proteins; D020815:Polyproteins; D011993:Recombinant Fusion Proteins; C577285:TTN protein, human; D011494:Protein Kinases