Interpretation of the complex karyotype and identification of a new 6p amplicon by integrated comparative genomic hybridization and fluorescence in situ hybridization on the U937-I cell line.
Molecular cytogenetics is helpful to identify complex and cryptic genomic changes in malignancy. Human leukemic cell lines are an important tool for advancements of biological research on malignant cells, one critical step being characterization of genomic changes. We used fluorescence in situ hybridization and comparative genomic hybridization to refine karyotypic interpretation of the diffuse histiocytic lymphoma derived U937-1 cell line. From this integrated approach, chromosome material involved in nine karyotypic markers and in unbalanced translocations could be identified. Moreover, a previously undetected amplicon emerged within band 6p21. The U937-I is a new in vitro model to study genome amplification and unknown recombinations in leukemic cells, such as those involving the centromeric region of chromosome 1.
Matteucci
Caterina
C
Hematology and Bone Marrow Transplantation Unit, University of Perugia, Policlinico Monteluce, Perugia, Italy.
La Starza
Roberta
R
Crescenzi
Barbara
B
Falzetti
Daniela
D
Romoli
Silvia
S
Emiliani
Carla
C
Orlacchio
Aldo
A
Marynen
Peter
P
Martelli
Massimo F
MF
Mecucci
Cristina
C
D000782:Aneuploidy; D002877:Chromosomes, Human / Q000235:genetics; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics / Q000648:ultrastructure; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics* / Q000648:ultrastructure; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D020298:U937 Cells / Q000648:ultrastructure*