title: Ex vivo PMA-induced respiratory burst and TNF-alpha secretion elicited from inflammatory cells on machined and porous blood plasma clot-coated titanium.
abstract: The release of inflammatory mediators around implants and normal wounds may differ due to the presence of the solid surface. In this study, machined and sub-micron porous titanium implants with and without a 100 nm thick blood plasma clot were inserted subcutaneously in rat for 3 or 24 h. The cell recruitment to the interfaces, in vivo secretion of TNF-alpha and the ex vivo PMA-induced production of reactive oxygen species were subsequently investigated. The thin plasma clot coating gave rise to an increased ex vivo PMA-stimulated oxygen radical production by implant-associated cells at both implantation times, and an increased cell recruitment at 24 h. The total TNF-alpha secretion was highest at sham sites and plasma clot-coated porous titanium at 24 h. After 24 h, the cell-type pattern in the exudate around the porous plasma-coated implant was more similar to that found at sham sites than that adjacent to the non-coated implants. No differences were observed between the machined Ti and the machined sub-micron porous Ti.
authors.affiliation: Department of Physics and Measurement Technology, Linköping University, Sweden.
authors.forename: Eva
authors.initials: E
authors.lastname: Jansson
authors.forename: Mia
authors.initials: M
authors.lastname: Källtorp
authors.forename: Peter
authors.initials: P
authors.lastname: Thomsen
authors.forename: Pentti
authors.initials: P
authors.lastname: Tengvall
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002470:Cell Survival; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D010100:Oxygen / Q000378:metabolism; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D016897:Respiratory Burst; D013755:Tetradecanoylphorbol Acetate / Q000737:chemistry*; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D014025:Titanium; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen