title: Molecular identification of alkaliphilic and halotolerant strain Bacillus sp. FTU as Bacillus pseudofirmus FTU.
abstract: The systematic position of the alkaliphilic and halotolerant strain Bacillus sp. FTU was refined in view of the comprehensive taxonomic revision of the group of alkaliphilic and alkalitolerant Bacillus strains. Sequence analysis of almost the entire 16S rRNA gene of Bacillus sp. FTU revealed 99.8% homology with two Bacillus pseudofirmus strains. Subsequent DNA-DNA hybridization analysis confirmed the close relationship of Bacillus sp. FTU with the type strain of B. pseudofirmus (the level of homology reached 86%). Results of physiological and biochemical characterizations relevant for the group clearly underlined the positioning of strain FTU within this species. It is therefore concluded that Bacillus sp. FTU represents a strain of the alkaliphilic species B. pseudofirmus and is to be renamed as B. pseudofirmus FTU. The phylogeny of different Bacillus species is discussed using N-terminal sequence homologies of some caa (3)-type oxidase subunits.
authors.affiliation: Department of Bioenergetics, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia.
authors.forename: Maria S
authors.initials: MS
authors.lastname: Muntyan
authors.forename: Tatiana P
authors.initials: TP
authors.lastname: Tourova
authors.forename: Anatolij M
authors.initials: AM
authors.lastname: Lysenko
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Kolganova
authors.forename: Dagmar
authors.initials: D
authors.lastname: Fritze
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Skulachev
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000145:classification / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S