Influence of aromatic compounds on biodegradation of [14C]-labeled xylan and mannan by the white-rot fungus Phlebia radiata.
Radiolabeled [14C]arabinoxylan from wheat meal and [14C]galactoglucomannan from red clover meal were prepared by using 14CO2 as a precursor. Twice as much mannan was mineralized than xylan after 14 days of incubation with Phlebia radiata. Low-molecular-weight phenolic compounds structurally related to lignin increased during mineralization of both hemicellulose fractions. Veratryl alcohol increased degradation of arabinoxylan by approximately 28.5%, whereas veratric acid increased it by only 9.0%. Vanillic acid and ferulic acid also stimulated degradation by 16.6% and 34.7%, respectively. Veratryl alcohol and ferulic acid increased degradation of galactoglucomannan by approximately 75%. Veratraldehyde in both cases repressed the degradation process (23.6% arabinoxylan, 43.8% galactoglucomannan). These results indicate that the degradation of hemicelluloses, e.g., xylan and mannan, by P. radiata is enhanced by addition of aromatic compounds.
Rogalski
J
J
Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland.
Cho
N S
NS
Zadora
J
J
Prendecka
M
M
Choma
A
A
Urbanik-Sypniewska
T
T
Leonowicz
A
A
D001547:Benzaldehydes / Q000494:pharmacology; D001673:Biodegradation, Environmental; D002250:Carbon Radioisotopes; D008351:Mannans / Q000378:metabolism*; D006109:Poaceae / Q000378:metabolism; D020072:Polyporales / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D014641:Vanillic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D014990:Xylans / Q000378:metabolism*
D001547:Benzaldehydes; D002250:Carbon Radioisotopes; D008351:Mannans; D011134:Polysaccharides; D014990:Xylans; C009333:veratric acid; C007916:hemicellulose; D014641:Vanillic Acid; C427725:veratraldehyde