Further characterization of equine foamy virus reveals unusual features among the foamy viruses.
Foamy viruses (FVs) are nonpathogenic, widely spread complex retroviruses which have been isolated in nonhuman primates, cattle, cats, and more recently in horses. The equine foamy virus (EFV) was isolated from healthy horses and was characterized by molecular cloning and nucleotide sequence analysis. Here, to further characterize this new FV isolate, the location of the transcriptional cap and poly(A) addition sites as well as the main splice donor and acceptor sites were determined, demonstrating the existence of the specific subgenomic pol mRNA, one specific feature of FVs. Moreover, similar to what has been described for the human foamy virus (HFV), the prototype of FVs, a replication-defective EFV genome was identified during persistent infection. At the protein level, the use of specific antibodies allowed us to determine the size and the subcellular localization of EFV Gag, Env, and Tas, the viral transactivators. While EFV Gag was detected in both the cytoplasm and the nucleus, EFV Env mainly localized in the Golgi complex, in contrast to HFV Env, which is sequestered in the endoplasmic reticulum. In addition, electron microscopy analysis demonstrated that EFV budding occurs at the plasma membrane and not intracellularly, as is the case for primate FVs. Interestingly, EFV Tas was detected both in the nucleus and the cytoplasm of Tas-transfected cells, in contrast to the strict nuclear localization of other FV Tas but similar to the equine infectious anemia virus Tat gene product. Taken together, our results reveal that this new FV isolate exhibits remarkable features among FVs, bringing new insights into the biology of these unconventional retroviruses.
Lecellier
Charles-Henri
CH
CNRS UPR9051, Hôpital Saint-Louis, 75475 Paris Cedex 10, France.
Neves
Manuel
M
Giron
Marie-Lou
ML
Tobaly-Tapiero
Joelle
J
Saïb
Ali
A
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015751:Genes, pol; D016679:Genome, Viral; D006736:Horses / Q000821:virology*; D008969:Molecular Sequence Data; D011061:Poly A / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012315:RNA Caps / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing; D012367:RNA, Viral / Q000378:metabolism; D012191:Retroviridae Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D016092:Spumavirus / Q000145:classification / Q000235:genetics* / Q000502:physiology*; D013347:Subcellular Fractions / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014764:Viral Proteins
D004268:DNA-Binding Proteins; D012315:RNA Caps; D012367:RNA, Viral; D012191:Retroviridae Proteins; D015534:Trans-Activators; D014764:Viral Proteins; C074061:bel1 protein, Human foamy virus; D011061:Poly A