title: FCLA chemiluminescence from sonodynamic action in vitro and in vivo.
abstract: In this work, the chemiluminescence method was engaged for the first time to detect the active oxygen species during sonodynamic action in vitro and in vivo. We used FCLA (3,7-dihydro-6-[4-[2-[N'-(5-fluoresceinyl)thioureido]-ethoxy]phenyl]-2-methylimidazo[1,2-a]pyrazin-3-one sodium salt), which can selectively react with singlet oxygen ((1)O(2)) or superoxide anion (O(2)(-)) to emit photons, to detect in real time oxygen free radical formation in the sonosensitization of hematoporphyrin derivative. The results show that (1)O(2) is involved in the sonosensitization. In in vivo experiments, a tumor-imaging method by sonodynamic chemiluminescence detection was established. This method could have potential applications in clinics for tumor diagnosis.
authors.affiliation: Institute of Laser Life Science, South China Normal University, Guangzhou 510631 China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: He
authors.forename: Da
authors.initials: D
authors.lastname: Xing
authors.forename: Guihong
authors.initials: G
authors.lastname: Yan
authors.forename: Ken ichi
authors.initials: Ki
authors.lastname: Ueda
mesh_terms: D005452:Fluoresceins; D016166:Free Radical Scavengers / Q000032:analysis; D006801:Humans; D007093:Imidazoles; D008163:Luminescent Measurements; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology*; D017785:Photons; D017382:Reactive Oxygen Species / Q000032:analysis*; D012680:Sensitivity and Specificity; D026082:Singlet Oxygen / Q000032:analysis; D013481:Superoxides / Q000032:analysis
chemical_list: D005452:Fluoresceins; D016166:Free Radical Scavengers; D007093:Imidazoles; D017382:Reactive Oxygen Species; D013481:Superoxides; C080213:3,7-dihydro-6-(4-(2-(N'-(5-fluoresceinyl)thioureido)ethoxy)phenyl)-2-methylimidazo-(1,2-a)pyrazin-3-one; D026082:Singlet Oxygen