Selenoprotein W is a glutathione-dependent antioxidant in vivo.
The function of selenoprotein W (Se-W) was investigated by cloning the corresponding cDNA from mouse brain and expressing it in CHO cells and H1299 human lung cancer cells. Overexpression of Se-W markedly reduced the sensitivity of both cell lines to H2O2 cytotoxicity. The intracellular peroxide concentration of the transfected cells was lower than that of the parental cells in the absence or presence of extracellular H2O2. The resistance to oxidative stress conferred by Se-W was dependent on glutathione. Expression of Se-W mutants in which selenocysteine-13 or cysteine-37 was replaced by serine did not confer resistance to H2O2, implicating these residues in the antioxidant activity of Se-W in vivo.
Jeong
Dae won
Dw
Laboratory of Cellular and Molecular Biochemistry, Graduate School of Biotechnology, Korea University, Seoul, South Korea.
Kim
Tae Soo
TS
Chung
Youn Wook
YW
Lee
Byeong Jae
BJ
Kim
Ick Young
IY
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D016466:CHO Cells / Q000187:drug effects / Q000378:metabolism; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D010545:Peroxides / Q000032:analysis / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D017279:Selenocysteine / Q000378:metabolism*; D051151:Selenoprotein W; D051140:Selenoproteins; D014162:Transfection; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D018076:DNA, Complementary; D010545:Peroxides; D011506:Proteins; C495240:SELENOW protein, human; D051151:Selenoprotein W; D051140:Selenoproteins; D017279:Selenocysteine; D006861:Hydrogen Peroxide; D005978:Glutathione