Tissue levels of matrix metalloproteinases in pulps and periapical lesions.
The purpose of this study was to evaluate the tissue levels of matrix metalloproteinase (MMP)-1, -2, -3 and their distributions in inflamed human dental pulps and periapical lesions. Samples were subjected to enzyme-linked immunosorbent assay and/or immunohistochemistry by using specific antibodies to MMP-1, -2, and -3. Results from enzyme-linked immunosorbent assay were analyzed by using the Mann-Whitney U test and presented as p values. The concentrations of MMP-1 in all experimental groups were significantly higher than in the control (p < 0.05). The acute pulpitis and control groups were significant different in terms of their MMP-2 levels (p < 0.05). The concentration of MMP-3 in acute pulpitis was significantly higher than the control and chronic pulpitis groups (p < 0.05). Immunohistochemically, MMP-1 and MMP-3 were localized in the infiltrating neutrophils, macrophages, and extracellular matrix of the acute pulpitis group. These results suggest that MMPs play an important role in the pulp tissue destruction of acute, inflamed pulp.
Shin
Su-Jung
SJ
Department of Conservative Dentistry, College of Dentistry, Seoul National University, Korea.
Lee
Jae-Il
JI
Baek
Seung-Ho
SH
Lim
Sung-Sam
SS
D000208:Acute Disease; D000911:Antibodies, Monoclonal; D002908:Chronic Disease; D003782:Dental Pulp / Q000201:enzymology; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000201:enzymology; D020781:Matrix Metalloproteinase 1 / Q000032:analysis*; D020778:Matrix Metalloproteinase 2 / Q000032:analysis*; D019278:Matrix Metalloproteinase 3 / Q000032:analysis*; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000201:enzymology; D010483:Periapical Diseases / Q000201:enzymology* / Q000473:pathology; D010484:Periapical Granuloma / Q000201:enzymology / Q000473:pathology; D011671:Pulpitis / Q000201:enzymology* / Q000473:pathology; D018709:Statistics, Nonparametric
D000911:Antibodies, Monoclonal; D019278:Matrix Metalloproteinase 3; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1