Typing of the locus DYS19 from DNA derived from fingernail clippings using PCR Concert rapid purification system.
DNA extracted from the fingernails of female victims of a violent or aggressive act may assist in the identification of the male. Sometimes with the current autosomal STR loci, however, the victim's profile may mask the perpetrator's DNA profile or the perpetrator's DNA may be substantially lower in quantity than that of the victim's DNA. Thus, under these conditions, no characterization is possible. In this paper, an alternative DNA extraction procedure was employed, and the application of an STR locus residing on the Y chromosome DYS19 was typed to allow for genetic characterization of the perpetrator in such cases.
Gangitano
David A
DA
DNA Section, Chemical Laboratory Division, Federal Police, Argentina.
Garófalo
Marisa G
MG
Juvenal
Guillermo J
GJ
Budowle
Bruce
B
Padula
Ricardo A
RA
D000328:Adult; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008297:Male; D009262:Nails / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D020080:Tandem Repeat Sequences / Q000235:genetics; D014754:Violence; D014998:Y Chromosome / Q000235:genetics*
D004247:DNA