[Effect of homologous peptides of differentiation factors HLDF and PEDF on preimplantation development of mice in vitro].
We studied the effect of synthetic peptides PEDF-6 and HLDF-6 on preimplantation development of mouse embryos in vitro. PEDF-6 peptide corresponds to fragment 351-356 and of pigment epithelium-derived differentiation factor (PEDF), while HLDF-6 peptide corresponds to fragment 84-89 of differentiation factor HLDF isolated from HL-60 cell line. Despite high homology, these peptides had different effects on the early development. PEDF-6 had no effect on the cleavage of 2-4-cell embryos but decelerated blastocyst formation from such embryos and disturbed their structure. In the presence of HLDF-6 the blastomeres divided more actively as compared to the control and a higher number of embryos developed to the blastocyst stage. The effects of both peptides were stage-specific: the affect the embryos at early cleavage stages and, apparently, determine their further development at that moment although do not directly affect formation of the blastocysts.
Sakharova
N Iu
NIu
Institute of Theoretical and Experimental Biology, Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290 Russia.
Kostanian
I A
IA
Lepikhova
T N
TN
Lepikhov
K A
KA
Malashenko
A M
AM
Navolotskaia
E V
EV
Tombran-Tink
J
J
Lipkin
V M
VM
D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D002454:Cell Differentiation; D002478:Cells, Cultured; D047108:Embryonic Development / Q000187:drug effects; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005136:Eye Proteins; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins / Q000737:chemistry*; D009414:Nerve Growth Factors; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D011247:Pregnancy; D011506:Proteins / Q000737:chemistry* / Q000494:pharmacology; D015843:Serpins / Q000737:chemistry* / Q000494:pharmacology; D000099174:Pigment Epithelium-Derived Factor
D005136:Eye Proteins; D009363:Neoplasm Proteins; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D011506:Proteins; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor; C091149:cell differentiation factor 8.2-kDa
Vliianie gomologichnykh peptidov factorov differentsirovki HLDF i PEDF na doimplantatsionnoe razvitie mysheĭ in vitro.