Chemically induced hairpin formation in DNA monolayers.
A naphthyridine dimer that binds specifically to G-G mismatches has been used to induce hairpin formation in oligonucleotides immobilized onto chemically modified gold surfaces. Surface plasmon resonance (SPR) imaging measurements of DNA microarrays were used to demonstrate that binding of the naphthyridine dimer to G-G mismatches within the stem portion of an immobilized 42-mer oligonucleotide could be used to induce hairpin formation that prevented hybridization of DNA complementary to the loop sequence. In addition, the selectivity of the naphthyridine dimer for G-G mismatches was verified through SPR imaging measurements of the hybridization adsorption of an 11-mer oligonucleotide to a four-component DNA array of zero- and single-base mismatch sequences.
Smith
Emily A
EA
Department of Chemistry, University of Wisconsin, Madison Wisconsin 53706, USA.
Kyo
Motoki
M
Kumasawa
Hiroyuki
H
Nakatani
Kazuhiko
K
Saito
Isao
I
Corn
Robert M
RM
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D006147:Guanine / Q000737:chemistry; D006860:Hydrogen Bonding; D008969:Molecular Sequence Data; D009287:Naphthyridines / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D020349:Surface Plasmon Resonance
D004277:DNA, Single-Stranded; D009287:Naphthyridines; D006147:Guanine; D004247:DNA