title: Hydrophobins in ectomycorrhizas: heterologous transcription of the Pisolithus HydPt-1 gene in yeast and Hebeloma cylindrosporum.
abstract: Hydrophobins are fungal cell wall proteins involved in aggregation of hyphae. Upon the development of the ectomycorrhizal symbiosis between tree roots and fungal hyphae, the transcripts of hydrophobin genes markedly accumulated. As the precise role of these proteins in symbiosis is not yet known, we develop heterologous expression system of the Pisolithus hydrophobin HYDPt-1. This gene has been introduced in Saccharomyces cerevisiae and in the ectomycorrhizal basidiomycete Hebeloma cylindrosporum. Introns were required for hydPt-1 transcript accumulation in the basidiomycete H. cylindrosporum. Heterologous transcript accumulation did not alter the phenotype of either species. The lack of altered phenotype resulted from the absence of HYDPt-1 polypeptide accumulation in transformed strains.
authors.affiliation: UMR INRA-UHP 1136 Interactions Arbres-Micro-organisimes, INRA-Nancy, Champenoux, France. tagu@nancy.inra.fr
authors.forename: D
authors.initials: D
authors.lastname: Tagu
authors.forename: R
authors.initials: R
authors.lastname: Marmeisse
authors.forename: Y
authors.initials: Y
authors.lastname: Baillet
authors.forename: S
authors.initials: S
authors.lastname: Rivière
authors.forename: B
authors.initials: B
authors.lastname: Palin
authors.forename: F
authors.initials: F
authors.lastname: Bernardini
authors.forename: A
authors.initials: A
authors.lastname: Méreau
authors.forename: G
authors.initials: G
authors.lastname: Gay
authors.forename: R
authors.initials: R
authors.lastname: Balestrini
authors.forename: P
authors.initials: P
authors.lastname: Bonfante
authors.forename: F
authors.initials: F
authors.lastname: Martin
mesh_terms: D001487:Basidiomycota / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*; D007438:Introns; D009687:Nuclear Proteins / Q000235:genetics*; D012331:RNA, Fungal / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D005656:Fungal Proteins; D009687:Nuclear Proteins; D012331:RNA, Fungal