Prevalence of Escherichia coli O157:H7 prophage-like sequences among German Salmonella enterica serotype Typhimurium phage types and their use in detection of phage type DT104 by the polymerase chain reaction.
A 1.6kb DNA fragment identified by random amplifiable polymorphic DNA differentiation (RAPD) from a Salmonella enterica serotype Typhimurium phage type DT104 isolate was used to investigate the prevalence of the region in 160 DT104 isolates, 83 other epidemiological important S. Typhimurium phage types and 20 strains selected from 17 other Salmonella serotypes. PCR screening tests using two different primer-sets derived from the RAPD fragment's nucleotide sequence showed that 76% of the 160 DT104 isolates investigated, including subtypes DT104A, DT104B, DT104B low, DT104H and DT104L, reacted positively. High sensitivity was shown for DT104 strains expressing at least the penta-resistance pattern ACSSuT (97% of 104 strains tested). DT104 susceptible strains showed only a sensitivity of 35% (17 strains tested). In contrast, 83% of the 83 strains from the other S. Typhimurium phage types reacted negatively. Strains from five out of the 17 other serotypes showed a positive signal with one primer-set. The other primer-set exhibited only a positive reaction with one S. Dublin isolate. The analysis of a 2415bp extended sequence revealed homologies to genes encoded by Escherichia coli O157:H7 prophages, suggesting that the described region contains genes of a prophage specific for DT104 and related phage types.
Malorny
Burkhard
B
Federal Institute for Health Protection of Consumers and Veterinary Medicine, Diedersdorfer Weg 1, Berlin, Germany.
Schroeter
Andreas
A
Bunge
Cornelia
C
Helmuth
Reiner
R
D000818:Animals; D001434:Bacteriophage Typing; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D019453:Escherichia coli O157 / Q000235:genetics*; D005858:Germany; D016366:Open Reading Frames / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012483:Salmonella Phages / Q000235:genetics*; D012486:Salmonella typhimurium / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial