A new lipophilic fluorescent probe for interaction studies of bioactive lipopeptides with membrane models.
The new fluorescent lipophilic moiety 11-[(7-amino-4-methyl-2-oxo-2H-1-benzopyran-3-acetyl)amino]undecanoic acid (AMCA-omegaAud-OH) was introduced by SPPS at the N-terminus of the immunodominant epitope GpMBP(74-85). FRET experiments using the new fluorescent lipopeptide demonstrate that the peptide interacts with much more affinity with the membrane compared to the lipid free analogue.
Peroni
Elisa
E
Dipartimento di Chimica Organica Ugo Schiff, Università degli Studi di Firenze, Polo Scientifico, I-50019 Sesto Fiorentino (FI), Italy.
Caminati
Gabriella
G
Baglioni
Piero
P
Nuti
Francesca
F
Chelli
Mario
M
Papini
Anna M
AM
D000818:Animals; D001665:Binding Sites; D004735:Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000276:immunology; D008051:Lipid Bilayers / Q000737:chemistry; D008074:Lipoproteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D008566:Membranes / Q000737:chemistry*; D008958:Models, Molecular; D019308:Palmitic Acid / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization
D005456:Fluorescent Dyes; D016056:Immunodominant Epitopes; D008051:Lipid Bilayers; D008074:Lipoproteins; D008563:Membrane Lipids; D019308:Palmitic Acid