Rapid selection of anti-hapten antibodies isolated from synthetic and semi-synthetic antibody phage display libraries expressed in Escherichia coli.
Antibody phage display libraries (Griffin and Tomlinson I) displaying antibody genes and maintained and amplified in Escherichia coli were used to isolate antibodies to the hapten target microcystin LR (1000 Da) conjugated to either bovine serum albumin or keyhole limpet haemocyanin. In competition enzyme-linked immunosorbent assay, bacterially expressed antibodies selected via the Griffin library showed at least 300 times greater sensitivity than those isolated from the Tomlinson library, for free microcystin. Bacterially expressed phage antibody libraries provide a rapid and relatively easy route for the selection of monoclonal antibodies specific for even the most difficult of antigenic targets such as free haptens.
Strachan
G
G
Haptogen Ltd, Polwarth Building, Foresterhill, Aberdeen AB25 2ZD, UK.
McElhiney
J
J
Drever
M R
MR
McIntosh
F
F
Lawton
L A
LA
Porter
A J R
AJ
D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D000918:Antibody Specificity; D001667:Binding, Competitive; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics*; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D006433:Hemocyanins; D006801:Humans; D008387:Marine Toxins; D052998:Microcystins; D019151:Peptide Library; D010456:Peptides, Cyclic / Q000737:chemistry / Q000276:immunology*; D012710:Serum Albumin, Bovine
D000911:Antibodies, Monoclonal; D006241:Haptens; D008387:Marine Toxins; D052998:Microcystins; D019151:Peptide Library; D010456:Peptides, Cyclic; D012710:Serum Albumin, Bovine; D006433:Hemocyanins; C057862:cyanoginosin LR; C032808:keyhole-limpet hemocyanin