title: Chymase participates in chronic dermatitis by inducing eosinophil infiltration.
abstract: An epicutaneous application of 2,4-dinitrofluorobenzene (DNFB) to a mouse ear caused a transient skin swelling, and the repetition of the challenge enlarged the contact dermatitis. The repeated challenge with DNFB also induced eosinophil infiltration on the application site. Administration of a chymase inhibitor significantly inhibited the ear swelling as well as eosinophil accumulation. An intradermal injection of human chymase to the mouse ear also elicited transient skin swelling and eosinophil infiltration, both of which were augmented in proportion to the number of injections. Human serum albumin and heat-inactivated chymase failed to induce such skin reactions, suggesting the participation of proteolytic activity of the enzyme. In addition, chymase stimulated eosinophil migration in vitro in a concentration-dependent manner. Taken together, these observations suggest that mast cell chymase may contribute to development of the DNFB-induced dermatitis, probably by promoting eosinophil infiltration. It is therefore possible that chymase plays a role in pathogenesis of chronic dermatitis such as atopic dermatitis.
authors.affiliation: Suntory Biomedical Research Limited, Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tomimori
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Muto
authors.forename: Harukazu
authors.initials: H
authors.lastname: Fukami
authors.forename: Kayo
authors.initials: K
authors.lastname: Saito
authors.forename: Chika
authors.initials: C
authors.lastname: Horikawa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tsuruoka
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saito
authors.forename: Namino
authors.initials: N
authors.lastname: Sugiura
authors.forename: Kyoko
authors.initials: K
authors.lastname: Yamashiro
authors.forename: Motoo
authors.initials: M
authors.lastname: Sumida
authors.forename: Saki
authors.initials: S
authors.lastname: Kakutani
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Fukuda
mesh_terms: D000287:Administration, Topical; D000485:Allergens / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity; D000818:Animals; D053818:Chymases; D003877:Dermatitis, Contact / Q000188:drug therapy / Q000201:enzymology* / Q000276:immunology; D004139:Dinitrofluorobenzene / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004431:Ear, External / Q000187:drug effects / Q000473:pathology; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004802:Eosinophilia / Q000188:drug therapy / Q000201:enzymology* / Q000276:immunology; D004804:Eosinophils / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D006968:Hypersensitivity, Delayed; D007271:Injections, Intradermal; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D011239:Prednisolone / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D012697:Serine Endopeptidases / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000485:Allergens; D004791:Enzyme Inhibitors; D011480:Protease Inhibitors; D011239:Prednisolone; D004139:Dinitrofluorobenzene; D012697:Serine Endopeptidases; D053818:Chymases