Distal regulatory regions restrict the expression of cis-linked genes to the tapetal cells.
The oleosin glycine-rich protein genes Atgrp-6, Atgrp-7, and Atgrp-8 occur in clusters in the Arabidopsis genome and are expressed specifically in the tapetum cells. The cis-regulatory regions involved in the tissue-specific gene expression were investigated by fusing different segments of the gene cluster to the uidA reporter gene. Common distal regulatory regions were identified that coordinate expression of the sequential genes. At least two of these genes were regulated spatially by proximal and distal sequences. The cis-acting elements (122 bp upstream of the transcriptional start point) drive the uidA expression to floral tissues, whereas distal 5' upstream regions restrict the gene activity to tapetal cells.
Franco
Luciana O
LO
Laboratório de Genética Molecular Vegetal, Departamento de Genética, Universidade Federal do Rio de Janeiro, C.P. 68011, Rio de Janeiro, Brazil.
de O  Manes
Carmem Lara
CL
Hamdi
Said
S
Sachetto-Martins
Gilberto
G
de Oliveira
Dulce E
DE
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D001937:Brassica / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005810:Multigene Family; D009928:Organ Specificity; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D016601:RNA-Binding Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D016601:RNA-Binding Proteins; C118496:GRP7 protein, Arabidopsis; C079353:oleosin protein, Arabidopsis