Spectrophotometric determination of serum nitrite and nitrate by copper-cadmium alloy.
A macro and micro assay for the spectrophotometric determination of serum nitrite and nitrate was developed. Nitrite/nitrate in biological samples can be estimated in a single step by this method. The principle of the assay is the reduction of nitrate by copper-cadmium alloy, followed by color development with Griess reagent (sulfanilamide and N-naphthylethylenediamine) in acidic medium. This assay is sensitive to 1 microM nitrate and is suitable for different biological fluids, including sera with a high lipid concentration. The copper-cadmium alloy used in the present method is easy to prepare and can completely reduce nitrate to nitrite in an hour. The present method provides a simple, cost-effective assay for the estimation of stable oxidation products of nitric oxide in biological samples.
Sastry
K V H
KV
Division of Physiology and Reproduction, Central Avian Research Institute, Uttar Pradesh, India.
Moudgal
R P
RP
Mohan
J
J
Tyagi
J S
JS
Rao
G S
GS
D000497:Alloys / Q000737:chemistry*; D000818:Animals; D002020:Buffaloes; D002104:Cadmium / Q000737:chemistry*; D002417:Cattle; D002645:Chickens; D003300:Copper / Q000737:chemistry*; D003370:Coturnix; D005029:Ethylenediamines; D016166:Free Radical Scavengers; D006041:Goats; D006801:Humans; D009566:Nitrates / Q000097:blood*; D009569:Nitric Oxide / Q000737:chemistry; D009573:Nitrites / Q000097:blood*; D010084:Oxidation-Reduction; D051381:Rats; D012680:Sensitivity and Specificity; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013424:Sulfanilamides; D013997:Time Factors
D000497:Alloys; D005029:Ethylenediamines; D016166:Free Radical Scavengers; C095000:Griess reagent; D009566:Nitrates; D009573:Nitrites; D013424:Sulfanilamides; D002104:Cadmium; D009569:Nitric Oxide; D003300:Copper