title: A set of cattle microsatellite DNA markers for genome analysis of riverine buffalo (Bubalus bubalis).
abstract: One hundred and eight microsatellite primer pairs, originally identified from cattle, were evaluated for their applicability in buffalo. Eighty-one primer pairs (75%) amplified discrete products, and of these, 61 pairs (56%) gave polymorphic band patterns on a panel of 25 buffaloes. The mean number of alleles per polymorphic marker was 4.50 +/- 0.20, and the mean heterozygosity per polymorphic marker was 0.66 +/- 0.02. Successful genotyping of buffaloes using cattle specific primers suggests that the latter can be a valuable resource for genome analysis in bubaline species.
authors.affiliation: National Bureau of Animal Genetic Resources, Karnal, India.
authors.forename: N
authors.initials: N
authors.lastname: Navani
authors.forename: P K
authors.initials: PK
authors.lastname: Jain
authors.forename: S
authors.initials: S
authors.lastname: Gupta
authors.forename: B S
authors.initials: BS
authors.lastname: Sisodia
authors.forename: S
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D017124:Conserved Sequence; D004247:DNA; D005819:Genetic Markers; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D005819:Genetic Markers; D004247:DNA