Effect of antimalarial drugs on plasmodia cell-free protein synthesis.
A cell-free system from Plasmodium falciparum able to translate endogenous mRNA was used to determine the effect of artemisinin, chloroquine and primaquine on the protein synthesis mechanism of the parasite. The antimalarial drugs did not inhibit the incorporation of [3H] methionine into parasite proteins even at concentrations higher than the ones found to strongly inhibit the parasite growth. Results clearly indicate that these compounds do not have a direct effect on protein synthesis activity of P. falciparum coded by endogenous mRNA.
Ferreras
Ana
A
Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad de Carabobo-Núcleo Aragua, Aragua, Venezuela.
Triana
Ledia
L
Sánchez
Erlinda
E
Herrera
Flor
F
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D037621:Artemisinins; D002738:Chloroquine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000378:metabolism; D011319:Primaquine / Q000494:pharmacology; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D015800:Protozoan Proteins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D012717:Sesquiterpenes / Q000494:pharmacology
D000962:Antimalarials; D037621:Artemisinins; D011500:Protein Synthesis Inhibitors; D015800:Protozoan Proteins; D012333:RNA, Messenger; D012717:Sesquiterpenes; D002738:Chloroquine; C031327:artemisinin; D011319:Primaquine