title: Immunodiagnosis of amoebiasis.
abstract: Serological studies were done on 127 cases using three different techniques namely indirect haemagglutination (IHA), indirect fluorescent antibody test (IFT) and counter immunoelectrophoresis (CIEP) to detect antiamoebic antibodies. All amoebic liver abscess cases showed significant titre of antibodies by all the three tests used. In the group of patients suffering from amoebic pathology of liver, 90.47 per cent were positive by IHA, 100 per cent by IFT and 85.71 per cent by CIEP respectively. Among amoebic dysentry and amoebic colitis cases 81.81 per cent and 80.64 per cent respectively were positive by IHA. The corresponding figures for IFT were 100 per cent and 74.19 per cent and for CIEP 90.90 per cent and 64.51 per cent respectively. Follow up study showed no significant fall in antibody titre in nine cases studied upto 10 weeks after treatment. Amoebic antigen could be detected in pus from all the nine cases with amoebic liver abscess by CIEP test.
authors.affiliation: Departments of Microbiology and Medicine, Maulana Azad Medical College, Associated Hospitals, New Delhi-110002.
authors.forename: U K
authors.initials: UK
authors.lastname: Baveja
authors.forename: R
authors.initials: R
authors.lastname: Kandhari
authors.forename: P S
authors.initials: PS
authors.lastname: Gupta
authors.forename: D S
authors.initials: DS
authors.lastname: Agarwal
mesh_terms: D000328:Adult; D000562:Amebiasis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000097:blood; D003378:Counterimmunoelectrophoresis; D004404:Dysentery, Amebic / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*; D008101:Liver Abscess, Amebic / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D008297:Male; D013492:Suppuration / Q000097:blood / Q000175:diagnosis / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan