Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon.
Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn5Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity.
Dacheux
Denis
D
Département de Biologie Moléculaire et Structurale, BBSI, UMR 5092 CNRS, CEA-Grenoble, CHU-Grenoble, Grenoble, France.
Epaulard
Olivier
O
de Groot
Arjan
A
Guery
Benoit
B
Leberre
Rozen
R
Attree
Ina
I
Polack
Benoit
B
Toussaint
Bertrand
B
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D003550:Cystic Fibrosis / Q000382:microbiology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D004195:Disease Models, Animal; D000071677:Histidine Kinase; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009504:Neutrophils / Q000382:microbiology; D009876:Operon / Q000502:physiology*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism; D018410:Pneumonia, Bacterial / Q000382:microbiology; D052899:Pore Forming Cytotoxic Proteins; D011494:Protein Kinases / Q000378:metabolism; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D011768:Pyruvate Dehydrogenase Complex / Q000235:genetics / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C095092:ExsA protein, bacteria; C412007:PopB protein, Ralstonia solanacearum; D052899:Pore Forming Cytotoxic Proteins; D011768:Pyruvate Dehydrogenase Complex; D015534:Trans-Activators; C453599:pyruvate dehydrogenase multienzyme complex E1; D007770:L-Lactate Dehydrogenase; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D011494:Protein Kinases; D000071677:Histidine Kinase; D010749:Phosphoprotein Phosphatases; C080406:protein phosphatase 4