title: Detection of Shiga toxins, intimin and enterohemolysin in Escherichia coli strains isolated from children in eastern Slovakia.
abstract: Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli virulence factors (shiga toxin 1 and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin; genes encoding shiga toxin 1 and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence of E. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains) was demonstrated.
authors.affiliation: Institute of Medical Microbiology, Medical Faculty, P.J. Safárik University, Kosice, Slovakia.
authors.forename: A
authors.initials: A
authors.lastname: Liptáková
authors.forename: L
authors.initials: L
authors.lastname: Siegfried
authors.forename: L
authors.initials: L
authors.lastname: Podracká
authors.forename: M
authors.initials: M
authors.lastname: Sabol
authors.forename: H
authors.initials: H
authors.lastname: Sehnálková
authors.forename: E
authors.initials: E
authors.lastname: Bogyiová
authors.forename: J
authors.initials: J
authors.lastname: Rosocha
authors.forename: M
authors.initials: M
authors.lastname: Kmetová
authors.forename: H
authors.initials: H
authors.lastname: Kerestesová
authors.forename: D
authors.initials: D
authors.lastname: Kotulová
mesh_terms: D018829:Adhesins, Bacterial / Q000032:analysis*; D002352:Carrier Proteins / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D004926:Escherichia coli / Q000737:chemistry* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D006460:Hemolysin Proteins / Q000032:analysis*; D006801:Humans; D016133:Polymerase Chain Reaction; D022603:Shiga Toxins / Q000032:analysis*; D018154:Slovakia
chemical_list: D018829:Adhesins, Bacterial; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D022603:Shiga Toxins; C066219:enterohemolysin; C073819:eaeA protein, E coli