[Dystrophin detection by immunofluorescent technique for diagnosing muscular dystrophy].
OBJECTIVE
To establish a specific technique for diagnosing and classifying Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD), facioscapulohumeral muscular dystrophy (FSHD) and neurologic dystrophy.


METHODS
Forty-seven cases were detected by immunofluorescence technique for analyzing dystrophin located in skeletal muscle cell membrane with the use of mouse monoclonal antibodies, goat and rabbit polyclonal antibodies.


RESULTS
The normal individuals showed ringed positive staining stripe around muscle fibers. Negative result of staining was seen in 16 DMD patients. Eleven BMD patients had discontinuous or a patchy positive staining pattern, and all of 10 FSHD and 10 neurological amyotrophic patients showed positive dystrophin staining.


CONCLUSIONS
Detecting dystrophin in the skeletal muscle cell membrane of muscular patients is an efficient technique for diagnosing and classifying various types of muscular dystrophy.
Wang
Xun
X
Department of Neurology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, 510080 P. R. China. czym@gzsums.edu.cn
Xie
Youmei
Y
Zhang
Cheng
C
Liu
Zhuoling
Z
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D016189:Dystrophin / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000473:pathology; D009136:Muscular Dystrophies / Q000175:diagnosis* / Q000378:metabolism; D020388:Muscular Dystrophy, Duchenne / Q000175:diagnosis / Q000378:metabolism; D020391:Muscular Dystrophy, Facioscapulohumeral / Q000175:diagnosis / Q000378:metabolism; D009468:Neuromuscular Diseases / Q000175:diagnosis / Q000378:metabolism
D016189:Dystrophin