title: A genome scan for quantitative trait loci affecting resistance to Trichostrongylus colubriformis in sheep.
abstract: A genome linkage scan was carried out using a resource flock of 1029 sheep in six half-sib families. The families were offspring of sires derived by crossing divergent lines of sheep selected for response to challenge with the intestinal parasitic nematode Trichostrongylus colubriformis. All animals in the resource flock were phenotypically assessed for worm resistance soon after weaning using a vaccination/challenge regime. After correcting for fixed effects using a least squares linear model the faecal egg count data obtained following the first challenge and the faecal egg count data obtained after the second challenge were designated Trait 1 and Trait 2, respectively. A total of 472 lambs drawn from the phenotypic extremes of the Trait 2 faecal egg count distribution were genotyped with a panel of 133 microsatellite markers covering all 26 sheep autosomes. Detection of quantitative trait loci (QTL) for each of the faecal egg count traits was determined using interval analysis with the Animap program with recombination rates between markers derived from an existing marker map. No chromosomal regions attained genome-wide significance for QTL influencing either of the traits. However, one region attained chromosome-wide significance and five other regions attained point-wise significance for the presence of QTL affecting parasite resistance.
authors.affiliation: CSIRO Livestock Industries, Locked Bag 1 Delivery Centre, Blacktown, NSW, Australia. ken.beh@csiro.au
authors.forename: K J
authors.initials: KJ
authors.lastname: Beh
authors.forename: D J
authors.initials: DJ
authors.lastname: Hulme
authors.forename: M J
authors.initials: MJ
authors.lastname: Callaghan
authors.forename: Z
authors.initials: Z
authors.lastname: Leish
authors.forename: I
authors.initials: I
authors.lastname: Lenane
authors.forename: R G
authors.initials: RG
authors.lastname: Windon
authors.forename: J F
authors.initials: JF
authors.lastname: Maddox
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002874:Chromosome Mapping; D005260:Female; D005819:Genetic Markers; D007113:Immunity, Innate; D008297:Male; D019655:Quantitative Trait, Heritable; D012756:Sheep; D012757:Sheep Diseases / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D014253:Trichostrongylosis / Q000235:genetics / Q000662:veterinary*
chemical_list: D005819:Genetic Markers