Investigations on factors that influence the moving neutralization reaction boundary method for capillary electrophoresis and isoelectric focusing.
We investigated several factors, such as temperature, current intensity (i), time (t) and the product (mA min mm(-2), viz., C mm(-2)) of i and t, etc., that obviously affect the moving neutralization reaction boundary method (MNRBM). The results manifest that the temperature and the product ti have a strong influence on the movement rate of the boundary. The data prove that about 0.6 C mm(-2) (being equivalent to 10 mA min mm(-2)) is a critical point. If the product ti is lower than the critical point, a good quantitative agreement exists between the observed and theoretical values, but if it is higher than the critical point, the agreements are poor. The optimized experimental conditions are: (1) 18-20 degrees C room temperature, (2) 0.6-0.8 mA mm(-2), (3) less than 10 mA min mm(-2), (4) 1% agarose gel, (5) daily prepared solution and gel containing NaOH. The optimized MNRBM is of benefit for the studies on MNRB itself, isoelectric focusing and capillary zone electrophoresis as will be partially shown in this paper.
Cao
Cheng-Xi
CX
College of Life Science and Biotechnology, Shanghai Jiaotong University, China. cxcao@mail1.sjut.edu.cn
Zhou
Shu-Lin
SL
Qian
Yi-Tai
YT
He
You-Zhao
YZ
Wang
Cheng-Run
CR
Zheng
Xiao-Yun
XY
Chen
Wen-Kui
WK
D002245:Carbon Dioxide; D019075:Electrophoresis, Capillary / Q000379:methods*; D007525:Isoelectric Focusing / Q000379:methods*; D015203:Reproducibility of Results; D012685:Sepharose; D013696:Temperature
D002245:Carbon Dioxide; D012685:Sepharose