title: Resinous perforation-repair materials inhibit the growth, attachment, and proliferation of human gingival fibroblasts.
abstract: The choice of repair material is one of the important factors in the prognosis of the endodontically treated tooth with a perforation defect. The cytotoxicity of perforation-repair materials must be investigated to ensure a safe biological response. The aim of this in vitro study was to evaluate the effect of resin-modified, glass-ionomer cement, compomer, and resin on human-gingival fibroblasts. Human gingival fibroblasts from crown lengthening surgery were cultured by using an explant technique with the consent of the patient. Cytotoxicity was judged by using an assay of tetrazolium bromide reduction. The results showed that resin-modified, glass-ionomer cement Fuji II LC, compomer Compoglass, and resin SpectrumTPH (TPH) were cytotoxic to primary human gingival fibroblast cultures by inhibiting cell growth and proliferation. TPH alone had an effect on cell attachment. It was found that TPH was the most cytotoxic repair material among those tested in all cultures. The toxicity decreased in the order of TPH>FLC>CG.
authors.affiliation: Department of Restorative Dentistry, Chung Shan Medical and Dental College Hospital, Taichung, Taiwan.
authors.forename: Fu-Mei
authors.initials: FM
authors.lastname: Huang
authors.forename: Kuo-Wei
authors.initials: KW
authors.lastname: Tai
authors.forename: Ming-Yung
authors.initials: MY
authors.lastname: Chou
authors.forename: Yu-Chao
authors.initials: YC
authors.lastname: Chang
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003124:Colorimetry; D004396:Coloring Agents; D020770:Compomers / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry; D003738:Dental Cements / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D005899:Glass Ionomer Cements / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D019279:Resin Cements / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D013053:Spectrophotometry; D013223:Statistics as Topic; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D001672:Biocompatible Materials; D004396:Coloring Agents; C105428:Compoglass; D020770:Compomers; D003188:Composite Resins; D003738:Dental Cements; C079765:Fuji II LC cement; D005899:Glass Ionomer Cements; D019279:Resin Cements; D012117:Resins, Synthetic; D012387:Root Canal Filling Materials; C420128:Spectrum composite resin; C113300:TPH hybrid; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue