Tumor necrosis factor alpha stimulates taurine uptake and transporter gene expression in human intestinal Caco-2 cells.
The effect of cytokines on the taurine uptake by human intestinal epithelial Caco-2 cells was investigated. Among the various cytokines tested, tumor necrosis factor alpha (TNF-alpha) markedly increased the taurine uptake by Caco-2 cells, resulting in an increase in the intracellular taurine level. TNF-alpha did not induce up-regulation of the taurine uptake in hepatic HepG2, renal human embryo kidney 293, and macrophage-like THP-1 cells. The uptake of glycine, L-leucine, and L-glutamic acid by Caco-2 cells was not affected by TNF-alpha. A kinetic analysis of the taurine uptake by TNF-alpha-treated Caco-2 cells suggests that this up-regulation was associated with both an increase in the amount of the taurine transporter (TAUT) and an increase in its affinity. TNF-alpha-treated cells showed a higher mRNA level of the TAUT than did the control cells.
Mochizuki
Tetsunosuke
T
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
Satsu
Hideo
H
Shimizu
Makoto
M
D018938:Caco-2 Cells / Q000187:drug effects* / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects*; D018698:Glutamic Acid / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D026901:Membrane Transport Proteins; D013654:Taurine / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology*; D015854:Up-Regulation; D000099247:Taurine Transporters
D002352:Carrier Proteins; D018698:Glutamic Acid; D005998:Glycine; D007930:Leucine; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D013654:Taurine; D014409:Tumor Necrosis Factor-alpha; D000099247:Taurine Transporters