Influence of boar and semen parameters on motility and acrosome integrity in liquid boar semen stored for five days.
Ninety ejaculates from a total of 76 AI boars were extended in Beltsville Thawing Solution (BTS). Boar identity, breed, weight of the ejaculate and sperm concentration were registered. Motility and acrosome integrity were assessed after storage at 16-18 degrees C for 6, 30, 54, 78, and 102 h. Storage time had a significant influence on both motility (p < 0.01) and acrosome integrity (p < 0.001). The Least Square Means for percentage of motility showed a small decline from 79.8% after 6 h of storage to 78.4% at 102 h. Motility at 78 and 102 h was significantly different from motility at 6 h (p < 0.05). The percentage of sperm cells with normal acrosomes declined throughout the experiment. The Least Square Means for 6, 30, 54, 78, and 102 h of storage were 93.9%, 90.6%, 88.0%, 84.8%, and 78.2%, respectively. The decrease in acrosome integrity from one storage time to the next was highly significant throughout the trial (p < 0.001). There was a significant influence of boar (p < 0.001) and sperm concentration (p < 0.01) on motility, while acrosome integrity was affected only by boar (p < 0.001). Breed of the boars and weight of the ejaculate did not influence the dependent variables.
Kommisrud
E
E
Department of Reproduction and Forensic Medicine, Norwegian School of Veterinary Science, Oslo, Norway. elisabeth.kommisrud@geno.no
Paulenz
H
H
Sehested
E
E
Grevle
I S
IS
D000177:Acrosome / Q000502:physiology*; D000818:Animals; D001947:Breeding; D015925:Cryopreservation / Q000662:veterinary; D008297:Male; D012661:Semen / Q000166:cytology* / Q000502:physiology*; D012662:Semen Preservation / Q000662:veterinary; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology; D013552:Swine / Q000502:physiology*; D013997:Time Factors