Sterile structural imaging of donor cornea by optical coherence tomography.
OBJECTIVE
The purpose of the study was to demonstrate a new noncontact method for sterile measurement of structure and thickness of donor cornea with use of optical coherence tomography (OCT).


METHODS
A commercially available OCT instrument designed for retinal measurements was used for noncontact assessment of human corneas. Structural changes occurring during organ culture were evaluated in 29 corneas. Comparison with histology was performed, and the ability of OCT to detect corneal scars and corneal thickness was investigated.


RESULTS
Corneal epithelium, stroma, and posterior curvature, as well as thickness, can be measured by standard OCT while the cornea remains in its storage bottle. Epithelial changes leading to a reduction of epithelial thickness, stromal structural changes, and hydration folds can be visualized. OCT scans correlate well with histology. Preexisting and developing corneal scars can be detected by OCT.


CONCLUSIONS
Corneal structural imaging can be performed under sterile conditions by OCT. This provides a method for improvement of corneal storage and a screening method for signs of photorefractive surgery and scarring in donor cornea.
Neubauer
Aljoscha S
AS
Department of Ophthalmology, Ludwig Maximilians University, Mathildenstrasse 8, 80336 Munich, Germany.
Priglinger
Siegfried G
SG
Thiel
Martin J
MJ
May
Christian-Albrecht
CA
Welge-Lüssen
Ulrich C
UC
D003315:Cornea / Q000033:anatomy & histology*; D003941:Diagnostic Techniques, Ophthalmological; D005125:Eye Banks; D006801:Humans; D007368:Interferometry; D008027:Light; D009924:Organ Culture Techniques; D009926:Organ Preservation; D014019:Tissue Donors; D014054:Tomography / Q000379:methods