title: Serum keratan sulphate as a cartilage metabolic marker in horses: the effect of exercise.
abstract: Keratan sulphate (KS) concentration in sera from resting horses and horses training daily on a racetrack was measured by an inhibition enzyme-linked immunosorbent assay (ELISA) using anti-equine KS antibody 1/14/16H9. For the in-training horses, serum KS concentrations in 2-year-old-horses was significantly higher than 3- or 4-year-old-horses. A higher concentration of serum KS was found in the in-training group than in the long-term resting group in 2-year-old-horses. Serum KS concentration increased remarkably immediately after training in healthy horses, and at 1, 5, 9 and 24 h after training remained at similar levels to the pre-training concentration. The results suggest that serum KS concentration could represent the situation of joint loading, induced by daily racetrack training, affecting the metabolic activities in joint cartilage.
authors.affiliation: Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan. okumuram@vetmed.hokudai.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Okumura
authors.forename: G H
authors.initials: GH
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Tagami
authors.forename: S
authors.initials: S
authors.lastname: Haramaki
authors.forename: T
authors.initials: T
authors.lastname: Fujinaga
mesh_terms: D000367:Age Factors; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002358:Cartilage, Articular / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D006736:Horses / Q000097:blood*; D007632:Keratan Sulfate / Q000032:analysis / Q000097:blood*; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology*
chemical_list: D015415:Biomarkers; D007632:Keratan Sulfate