Cycloaliphatic epoxy resin coating for capillary electrophoresis.
Coating the interior surface of a fused-silica capillary with a polymeric material has long been used in capillary electrophoresis (CE) to reduce or eliminate electroosmotic flow and suppress adsorption. A cycloaliphatic epoxide-based resin was bonded to silane treated capillaries and crosslinked with a curing agent. The epoxy resin coating significantly reduced electroosmotic flow over a pH range of 3-10. This coating was sufficiently hydrophilic to suppress protein adsorption. The epoxy resin coated capillary was used to separate several acidic and basic proteins and peptides. Separation efficiencies greater than 400,000 theoretical plates were achieved. The relative standard deviations in migration times for proteins were <0.8%. Speed and simplicity are important advantages of the coating procedure compared to other published coating methods.
Shah
Roopa S
RS
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602-5700, USA.
Wang
Qinggang
Q
Lee
Milton L
ML
D000595:Amino Acid Sequence; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004853:Epoxy Resins / Q000737:chemistry*; D010449:Peptide Mapping; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
D004853:Epoxy Resins; D011506:Proteins