Molecular cloning of rabbit organic cation transporter rbOCT2 and functional comparisons with rbOCT1.
Multiple organic cation transporters (OCTs) are present in rabbit kidney and may play different functional roles. We cloned rabbit OCT2 (rbOCT2) and compared its function with that of rabbit OCT1 (rbOCT1). In transiently transfected COS-7 cells, rbOCT1 and rbOCT2 mediated uptake of [3H]tetraethylammonium (TEA) with K(t) values of 188 and 125 microM, respectively. n-Tetraalkylammonium compounds showed similar affinities for the two homologs, with IC50 values for inhibition of OCT1- and OCT2-mediated [3H]TEA transport, respectively, of 4,538 and 1,395 microM for tetramethylammonium, 88.5 and 3.9 microM for tetrapropylammonium, 13.9 and 5.3 microM for tetrabutylammonium, and 8.8 and 7.6 microM for tetrapentylammonium. However, the transporters had very different affinities for cimetidine (CIM): IC50 of 916 and 5.7 microM for rbOCT1 and rbOCT2, respectively. CIM inhibition of TEA uptake into single S2 segments of rabbit proximal tubule was used to estimate the contributions of OCT1 and OCT2 to basolateral organic cation uptake. The median IC50 for CIM inhibition of TEA uptake was 12.3 microM, suggesting that OCT2 is the major contributor to basolateral organic cation transport in the S2 segment of proximal tubule in rabbit kidney.
Zhang
Xiaohong
X
Department of Physiology, College of Medicine, University of Arizona, Tucson, Arizona 85724, USA.
Evans
Kristen K
KK
Wright
Stephen H
SH
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D019556:COS Cells; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D002412:Cations / Q000378:metabolism; D003001:Cloning, Molecular; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008969:Molecular Sequence Data; D026902:Potassium Channel Blockers / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D019789:Tetraethylammonium / Q000494:pharmacology; D014316:Tritium
D027682:Cation Transport Proteins; D002412:Cations; D026902:Potassium Channel Blockers; D012333:RNA, Messenger; D014316:Tritium; D019789:Tetraethylammonium