Inhibitory effects of lemon grass (Cymbopogon citratus, Stapf) extract on the early phase of hepatocarcinogenesis after initiation with diethylnitrosamine in male Fischer 344 rats.
Effects of lemon grass extract (LGE) on hepatocarcinogenesis were examined in male Fischer 344 rats, administered diethylnitrosamine (DEN) at three weekly intraperitoneal doses of 100 mg/kg body weight and partially hepatectomized at the end of week 5. LGE was given at dietary concentrations of 0, 0.2, 0.6 or 1.8% from the end of week 4 for 10 weeks. All rats were sacrificed at the end of week 14. LGE reduced the number of putatively preneoplastic, glutathione S-transferase placental form-positive lesions and the level of oxidative hepatocyte nuclear DNA injury, as assessed in terms of 8-hydroxydeoxyguanosine production. In contrast, LGE did not affect the size of the preneoplastic lesions, hepatocyte proliferative activity, activities of phase II enzymes or hepatocyte extra-nuclear oxidative injury. These results suggest inhibitory effects of LGE on the early phase hepatocarcinogenesis in rats after initiation with DEN.
Puatanachokchai
Rawiwan
R
Department of Oncological Pathology, Cancer Center, Nara Medical University, Kashihara, 634-8521, Nara, Japan.
Kishida
Hideki
H
Denda
Ayumi
A
Murata
Nao
N
Konishi
Yoichi
Y
Vinitketkumnuen
Usanee
U
Nakae
Dai
D
D000818:Animals; D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D004052:Diethylnitrosamine; D005982:Glutathione Transferase / Q000032:analysis; D006498:Hepatectomy; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D019684:Magnoliopsida; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D016588:Anticarcinogenic Agents; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D004052:Diethylnitrosamine; D005982:Glutathione Transferase