Minimal number of chromatographic test parameters for the characterisation of reversed-phase liquid chromatographic stationary phases.
This paper focuses on the classification or differentiation of RP-HPLC columns based on measured chromatographic properties. A chemometric study has been conducted on a published data set consisting of 85 RP-HPLC columns and on a data set consisting of 47 self-tested columns. Principal component analysis enables determination of the number of parameters necessary for a rational differentiation. The results show that reducing the number of parameters for such differentiation still allows classification of the columns just as a higher number did. It is shown that three test parameters produce a classification similar to that obtained with five parameters.
Iványi
Timea
T
Vrije Universiteit Brussel, Department of Pharmaceutical and Biomedical Analysis, Belgium.
Vander Heyden
Yvan
Y
Visky
Dóra
D
Baten
Peggy
P
De Beer
Jacques
J
Lázár
István
I
Massart
D L
DL
Roets
Eugène
E
Hoogmartens
Jos
J
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration