title: Expression of Human Single Chain Interleukin 12 Gene in Drosophila Cells.
abstract: The hscIL-12 gene(no signal sequence), amplified by PCR, was subcloned into an expressing vector pMT/Bip/V5. The recombinant plasmid was transferedinto Drosophila cell line S2. The secreted hscIL-12 fusion protein was identified by Western blot, and the bioactivity of hscIL-12 was investigated by lymphocyte proliferation assay. The results showed hscIL-12 expressed in S2 cells could stimulate lymphocyte proliferation in dose-dependent manner. hscIL-12 has bioactivity identical to natural interleukin 12. These findings suggest that hscIL-12 is promising in theoretical reseach and in clinical use.
authors.affiliation: Central Laboratory of Shanghai No.1 Hospital, Shanghai 200080, China. jinli73@yahoo.com
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.forename: Xu
authors.initials: X
authors.lastname: Qi
authors.forename: Bao-Chang
authors.initials: BC
authors.lastname: Lai
authors.forename: Lü-Sheng
authors.initials: LS
authors.lastname: Si
authors.forename: Yi-Li
authors.initials: YL
authors.lastname: Wang