Differential incorporation of 1-deoxy-D-xylulose into (3S)-linalool and geraniol in grape berry exocarp and mesocarp.
In vivo feeding experiments with [5,5-(2)H(2)]mevalonic acid lactone (MVL) and [5,5-(2)H(2)]-1-deoxy-D-xylulose (DOX) indicate that the novel mevalonate-independent 1-deoxy- D-xylulose 5-phosphate/2C-methyl- D-erythritol 4-phosphate (DOXP/MEP) pathway is the dominant metabolic route for monoterpene biosynthesis in grape berry exocarp and mesocarp and in grape leaves. The highly uneven distribution of the monoterpene alcohols (3S)-linalool and geraniol between leaves, berry exocarp and berry mesocarp can be attributed to a compartmentation of monoterpene metabolism. In grape berries incorporation of [5,5-(2)H(2)]-DOX into geraniol is mainly restricted to the exocarp, whereas (3S)-linalool biosynthesis can be detected in exocarp as well as in mesocarp tissue. The results demonstrate that grape berries exhibit an autonomic monoterpene biosynthesis via the novel DOXP/MEP route throughout the ripening process.
Luan
Fang
F
Institut für Lebensmittelchemie, Biozentrum, Johann Wolfgang Goethe-Universität, Marie-Curie-Strasse 9, D-60439, Frankfurt, Germany.
Wüst
Matthias
M
D000080462:Acyclic Monoterpenes; D002845:Chromatography; D003903:Deuterium; D005638:Fruit / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D039821:Monoterpenes / Q000737:chemistry / Q000378:metabolism*; D018514:Plant Structures / Q000378:metabolism; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism*; D027843:Vitis / Q000737:chemistry / Q000378:metabolism*; D014996:Xylulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*
D000080462:Acyclic Monoterpenes; D039821:Monoterpenes; D013729:Terpenes; D014996:Xylulose; D003903:Deuterium; C018584:linalool; C007836:geraniol