title: Characterization of B-1b cells as antigen presenting cells in the immune response to gp43 from Paracoccidioides brasiliensis in vitro.
abstract: Antigen presentation is an essential stage in the development of immune response to a specific antigen. This response can lead to the production of antibodies and/or effector T lymphocyte activation. Macrophages, dendritic cells and B-lymphocytes, among others, act as antigen presenting cells. B-lymphocytes capture antigenic particles through a surface receptor of IgM nature. The interaction IgM-antigen leads to endocytosis of the complex and antigen processing which culminates in presentation of the antigen on the cell surface associated with a class II MHC molecule. At least three B cell subsets, B-1a (Ly-1B), B-1b and B-2, are present in the mouse periphery. B-1a and B1-b cells represent a small population in the adult spleen and are abundant in the peritonial and pleural cavities. It has been demonstrated in our laboratory that B-1b cells spontaneously proliferated in stationary cultures of adherent peritonial cells. Further, that these cells migrate to a non-specific inflammatory focus. Based on these findings, we investigated whether these cells are antigen presenting cells in vitro using as antigenic stimulus gp43 from Paracoccidioides brasiliensis. Results showed that B1-b cells express constitutively high levels of class II MHC and costimulatory molecules inducing an efficient proliferation of gp43 sensitized T lymphocytes.
authors.affiliation: Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Rua Botucatu, 862/4 andar, 04023-900 São Paulo, Brazil.
authors.forename: Ana Flavia Grandi
authors.initials: AF
authors.lastname: Vigna
authors.forename: Luiz Claudio
authors.initials: LC
authors.lastname: Godoy
authors.forename: Sandro
authors.initials: S
authors.lastname: Rogerio de Almeida
authors.forename: Mario
authors.initials: M
authors.lastname: Mariano
authors.forename: José Daniel
authors.initials: JD
authors.lastname: Lopes
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000946:Antigens, Fungal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D018956:CD5 Antigens; D002460:Cell Line; D005260:Female; D005656:Fungal Proteins; D005802:Genes, MHC Class II; D006023:Glycoproteins / Q000276:immunology*; D066298:In Vitro Techniques; D051379:Mice; D009844:Oligosaccharides / Q000276:immunology*; D010228:Paracoccidioides / Q000276:immunology*; D010641:Phenotype
chemical_list: C103180:43 kDa protein, Paracoccidioides; D000946:Antigens, Fungal; D018956:CD5 Antigens; D005656:Fungal Proteins; D006023:Glycoproteins; D009844:Oligosaccharides