title: Comparison of the variables affecting the recovery of DNA from common drinking containers.
abstract: As the boundaries of forensic DNA profiling continue to expand, less obvious sources of biological evidence are being collected at crime scenes for DNA profiling. One example is the recovery of biological evidence from common drink containers, such as bottles and cans, which have been found at crime scenes. There are many variables that may have an impact on recovering a DNA profile from such exhibits. In this research, the effects of person to person variation, time, type of drink (including alcoholic and non-alcoholic beverages), and type of drink container, were assessed for their impact on the major analytical outcomes of the DNA process. The results show that the alpha-amylase activity varies from individual to individual and is reduced in the presence of some alcoholic drinks. A reasonable DNA yield was obtained from all samples, however, the concentrations exhibited significant person to person variation. The type of drink container influenced the DNA yield with cans giving a higher yield than bottles of the same drink type. To a reduced extent the presence or absence of alcohol affected the overall DNA yield and when partial or failed DNA profiles were produced they were more likely to be associated with alcoholic drinks than non-alcoholic drinks.
authors.affiliation: Institute of Environmental Science and Research Ltd, Private Bag 92-021, Auckland, New Zealand. jelena.abaz@esr.cri.nz
authors.forename: Jelena
authors.initials: J
authors.lastname: Abaz
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Walsh
authors.forename: James M
authors.initials: JM
authors.lastname: Curran
authors.forename: Delia S
authors.initials: DS
authors.lastname: Moss
authors.forename: Judi
authors.initials: J
authors.lastname: Cullen
authors.forename: Jo-Anne
authors.initials: JA
authors.lastname: Bright
authors.forename: Gillian A
authors.initials: GA
authors.lastname: Crowe
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Cockerton
authors.forename: Timothy E B
authors.initials: TE
authors.lastname: Power
mesh_terms: D001628:Beverages; D016172:DNA Fingerprinting; D004326:Drinking; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008297:Male; D015999:Multivariate Analysis; D019064:Product Packaging; D012463:Saliva / Q000737:chemistry* / Q000201:enzymology; D000516:alpha-Amylases / Q000032:analysis*
chemical_list: D000516:alpha-Amylases