title: Association of IL-10 expression by mucosal lymphocytes with increased expression of Cryptosporidium parvum epitopes in infected epithelium.
abstract: The objective of this study was to determine whether changes in the ileal intraepithelial lymphocyte (TEL) phenotype and function occurred prior to development of diarrhea in Cryptosporidium parvum-infected calves. Calves were orally inoculated with 10(8) oocysts and maintained in enteric pathogen-free conditions until their use in experiments. Age-matched uninfected calves were used for comparisons. Ileal IELs were isolated and phenotyped to determine whether changes in lymphocyte population dynamics had occurred by 3 days postinoculation (PI). Ex vivo reverse transcriptase-polymerase chain reaction of messenger ribonucleic acid (mRNA) from IELs from infected calves was compared with controls to determine whether changes in cytokine expression had occurred by 3 days PI. No significant changes in lymphocyte population dynamics were documented, however, IELs isolated from 4 out of 8 infected calves, but not from 8 out of 8 control calves, expressed mRNA for interleukin-10 (IL-10). IL-10 expression by IELs was associated with the expression of a significantly larger (P < 0.001) proportion (0.75) of monoclonal antibody-defined C. parvum epitopes within infected ileal epithelium, as compared with a much smaller proportion (0.30) of epitopes with IL-10 lymphocytes. The results suggest that a temporal association exists between the expression of IL-10 by ileal IELs and the expression of C. parvum antigens in infected calf epithelium prior to development of cryptosporidiosis.
authors.affiliation: Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan 66506-5705, USA. cwyatt@vet.ksu.edu
authors.forename: Carol R
authors.initials: CR
authors.lastname: Wyatt
authors.forename: Wendy J
authors.initials: WJ
authors.lastname: Barrett
authors.forename: E Joan
authors.initials: EJ
authors.lastname: Brackett
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Schaefer
authors.forename: Michael W
authors.initials: MW
authors.lastname: Riggs
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000953:Antigens, Protozoan / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology; D002454:Cell Differentiation / Q000276:immunology; D003457:Cryptosporidiosis / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D016785:Cryptosporidium parvum / Q000235:genetics / Q000276:immunology*; D003967:Diarrhea / Q000276:immunology / Q000469:parasitology / Q000662:veterinary; D000939:Epitopes / Q000276:immunology*; D005786:Gene Expression Regulation; D007150:Immunohistochemistry / Q000662:veterinary; D016753:Interleukin-10 / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology* / Q000469:parasitology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D000953:Antigens, Protozoan; D000939:Epitopes; D012333:RNA, Messenger; D016753:Interleukin-10