title: Expression of G1 phase cyclins in human gastric cancer and gastric mucosa of first-degree relatives.
abstract: The aim of this study was to investigate the expression of D-type cyclins and cyclin E in gastric cancer patients (N = 34), in healthy first-degree relatives of gastric cancer patients (N = 29), and in control subjects (N = 18). Expression of cyclins D1, D2, D3, and E was determined by RT-PCR. Localization of cyclin expression was determined by immunohistochemistry. Expression of cyclins D2, D3, and E was more frequently detected in tumor tissue compared with tumor-free gastric mucosa (P < 0.05) and was associated with the presence of intestinal metaplasia. In contrast, cyclin D1 was frequently expressed in both tumor- and tumor-free tissue. Cyclin D3 expression was more frequently detected in the antrum mucosa of first-degree relatives compared to controls (P < 0.01) and was associated with the presence of Helicobacter pylori. Our data suggest that deregulation of G1 phase cyclins may play a role in gastric carcinogenesis, and may point to the presence of molecular alterations in individuals at an increased risk for gastric cancer.
authors.affiliation: Medical Department I, Gastroenterology, Hematology & Oncology, Technical University Hospital, Dresden, Germany. miehlke@mk1.med.tu-dresden.de
authors.forename: Stephan
authors.initials: S
authors.lastname: Miehlke
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Matthias
authors.initials: M
authors.lastname: Ebert
authors.forename: Daniel
authors.initials: D
authors.lastname: Szokodi
authors.forename: Michael
authors.initials: M
authors.lastname: Vieth
authors.forename: Eberhard
authors.initials: E
authors.lastname: Kuhlisch
authors.forename: Rene
authors.initials: R
authors.lastname: Buchcik
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schimmin
authors.forename: Ursula
authors.initials: U
authors.lastname: Wehrmann
authors.forename: Peter
authors.initials: P
authors.lastname: Malfertheiner
authors.forename: Gerhard
authors.initials: G
authors.lastname: Ehninger
authors.forename: Ekkehard
authors.initials: E
authors.lastname: Bayerdörffer
authors.forename: Manfred
authors.initials: M
authors.lastname: Stolte
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019938:Cyclin D1 / Q000378:metabolism*; D019927:Cyclin E / Q000378:metabolism*; D005260:Female; D016193:G1 Phase / Q000502:physiology*; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism*
chemical_list: D019927:Cyclin E; D019938:Cyclin D1