Hypertonic sabouraud broth as a simple and powerful test for Candida dubliniensis screening.
We developed a new screening test for C. dubliniensis based on its inability to grow on Sabouraud dextrose broth with 6.5% NaCl. A total of 266 clinical yeast isolates and 3 reference strains were tested, including 250 C. albicans and 19 C. dubliniensis strains. All C. albicans isolates tested exhibited significant growth on hypertonic Sabouraud broth up to 96 h, while, all C. dubliniensis isolates did not exhibit any visually detectable growth during the same period.
Alves
Sydney Hartz
SH
Department of CIinical and Toxicological Analysis, Universidade Federal de Santa Maria, RS, Santa Maria, Brazil.
Milan
Eveline Pipolo
EP
de Laet Sant'Ana
Priscilla
P
Oliveira
Loiva O
LO
Santurio
Janio M
JM
Colombo
Arnaldo Lopes
AL
D002175:Candida / Q000145:classification* / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D002176:Candida albicans / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D002177:Candidiasis / Q000175:diagnosis* / Q000382:microbiology*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology*; D005947:Glucose; D006801:Humans; D006982:Hypertonic Solutions; D016533:Mycological Typing Techniques / Q000379:methods*; D009994:Osmolar Concentration; D012680:Sensitivity and Specificity; D012965:Sodium Chloride; D013045:Species Specificity; D013997:Time Factors
D003470:Culture Media; D006982:Hypertonic Solutions; D012965:Sodium Chloride; D005947:Glucose