title: Expression of tenascin-C long isoforms is induced in the hypothalamus by FGF-1.
abstract: Fibroblast growth factor (FGF)-1 modulates various brain functions, such as the hypothalamic control of feeding. In the rat, we examined the effect of intracerebroventricularly infused FGF-1 on the hypothalamic expression of tenascin-C, a selective mediator of neuron-glial interaction. In situ hybridization revealed little tenascin-C mRNA expression in control brains, but greatly increased expression in ependymal cells around the third ventricle in the FGF-1-infused rats. Reverse transcription-linked PCR analysis of hypothalamic mRNA revealed an FGF-1-induced expression not of the shortest isoform of tenascin-C, but of the long isoforms (with additional fibronectin type III-domain insertions). Quantitative analysis by real time PCR revealed that this induction was transient and dose-dependent. Specific modulation of hypothalamic neuron-glial interactions by tenascin-C may mediate FGF-1-induced feeding suppression.
authors.affiliation: Gene Discovery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), AIST Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Seigo
authors.initials: S
authors.lastname: Suzuki
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Li
authors.forename: Mitsushi
authors.initials: M
authors.lastname: Ikemoto
authors.forename: Toru
authors.initials: T
authors.lastname: Imamura
mesh_terms: D000818:Animals; D001069:Appetite Regulation / Q000187:drug effects / Q000502:physiology; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004805:Ependyma / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015676:Osteonectin / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D019063:Tenascin / Q000235:genetics*; D019700:Thrombospondin 1 / Q000235:genetics; D013997:Time Factors
chemical_list: D015676:Osteonectin; D020033:Protein Isoforms; D012333:RNA, Messenger; D019063:Tenascin; D019700:Thrombospondin 1; D016220:Fibroblast Growth Factor 1