Ethanol signals for apoptosis in cultured skin cells.
Ethanol is commonly used in cosmetic and pharmaceutical preparations. To test whether ethanol may cause apoptosis in skin cells, we treated A431 epidermoid skin cells and neonatal human primary skin cells with different concentrations of ethanol, for different time periods. Ethanol was toxic to cells in both a dose- and time-dependent manner and increased the percentage of cells undergoing apoptosis. Treatment of cells with 40 and 100 mM ethanol increased release of the proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) into culture medium and increased its expression in cells. The TNF-alpha was toxic to A431 epidermoid skin cells at concentrations similar to those released by cells on exposure to ethanol. Ethanol-treated cells examined by electron microscopy showed organelle damage, condensed chromatin, and apoptotic bodies. Therefore, even at low concentrations, ethanol may induce apoptosis in skin cells by enhancing the effects of TNF-alpha.
Neuman
Manuela G
MG
Departments of Clinical Pharmacology, Dermatology, Medicine, and Laboratory Medicine, University of Toronto, M5S 1A1, Toronto, Ontario, Canada. manuela@sten.sunnybrook.utoronto.ca
Haber
Julia A
JA
Malkiewicz
Izabella M
IM
Cameron
Ross G
RG
Katz
Gady G
GG
Shear
Neil H
NH
D000704:Analysis of Variance; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007231:Infant, Newborn; D008297:Male; D008854:Microscopy, Electron; D012867:Skin / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000494:pharmacology
D016207:Cytokines; D007106:Immune Sera; D014409:Tumor Necrosis Factor-alpha; D000431:Ethanol