title: [Glomerular morphologic protection after acute ischemia through the administration of Surgiran: (PGE1 and Alfadex, glucose cyclic oligomer). Morphometric study].
abstract: OBJECTIVE
  To quantify the morphologic protection of PGE1 on renal glomerulus following normothermic acute renal ischemia.
  
  
  METHODS
  This experimental study was carried out in 48 adult male Wistar rats. A simple right nephrectomy was performed in all rats. The rats were divided into two groups: one group was perfused with PGE1 and the other group with saline solution immediately after acute renal ischemia, which was produced by non-traumatic vascular clamping. The ischemia times were 15 and 60 minutes. The recovery period ranged from 24 hours to 7 days. After this period the animals were anesthetized and sacrificed. Histological and morphometric analyses of the right kidneys of the control group and the left kidneys of the study group were performed.
  
  
  RESULTS
  The mortality was 31% (18.5% for the group perfused with PGE1 and 11.4% for the group perfused with saline solution). Kidneys of rats perfused with saline solution weighed more than the kidneys of rats perfused with PGE1 (1.771 +/- 0.455 and 1.55 +/- 0.34, respectively). Acute tubular necrosis was observed after 60 minutes' ischemia and was more evident in the saline than in the PGE1 group. The morphometric study showed no significant differences between the control (normal) and the PGE1 group for glomerular diameter (p < or = 0.101), sphericity factor (p < or = 0.239), glomerular perimeter (p < or = 0.092) and glomerular volume (p < or = 0.059). However, significant differences were found between the control and the saline perfusion group for area (p < or = 0.000), diameter (p < or = 0.000), perimeter (p < or = 0.000) and volume (p < or = 0.000).
  
  
  CONCLUSIONS
  Kidney weight after ischemia increased more in the saline than in the PGE1-treated group since the edema in the renal parenchyma is attenuated by the anti-inflammatory and cytoprotective effects of PGE1. The morphometric study showed that in comparison to saline, PGE1 exerts a cytoprotective effect, although it is not considerable.
authors.affiliation: Servicio de Urología, Hospital Universitario Río Hortega, Valladolid, España. cconde@airtel.net
authors.forename: Consuelo
authors.initials: C
authors.lastname: Conde Redondo
authors.forename: Vicente
authors.initials: V
authors.lastname: Herreros Rodríguez
authors.forename: Luis Antonio
authors.initials: LA
authors.lastname: Rodríguez-Toves
authors.forename: Javier
authors.initials: J
authors.lastname: Estabanez Zarranz
authors.forename: José María
authors.initials: JM
authors.lastname: Martínez Sagarra
authors.forename: Carlos
authors.initials: C
authors.lastname: Vaquero
mesh_terms: D000208:Acute Disease; D000527:Alprostadil / Q000627:therapeutic use*; D000818:Animals; D003505:Cyclodextrins / Q000627:therapeutic use*; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D007511:Ischemia / Q000517:prevention & control*; D007668:Kidney / Q000033:anatomy & histology / Q000098:blood supply*; D007678:Kidney Glomerulus / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D047391:alpha-Cyclodextrins
chemical_list: D003505:Cyclodextrins; D047391:alpha-Cyclodextrins; C051518:OP 41483-alpha-cyclodextrin; D011464:Epoprostenol; D000527:Alprostadil
vernacular_title: Protección morfológica glomerular tras isquemia aguda mediante la administración de Surgiran: (PGE1 y Alfadex, oligómero cíclico de la glucosa). Estudio morfométrico.