title: Tenascins are associated with lipid rafts isolated from mouse brain.
abstract: Lipid rafts are microdomains of the plasma membrane which are enriched in glycosphingolipids and specific proteins. The reported interactions of several raft-associated proteins (such as, e.g., F3) with tenascin C and tenascin R prompted us to consider that these oligomeric multidomain glycoproteins of the extracellular matrix (ECM) could associate with rafts. Here, we show punctate immunocytochemical distributions of tenascin C (TN-C) and tenascin R (TN-R) at the membrane surface of neural cells resembling the pattern reported for raft-associated proteins. Moreover, cholesterol depletion with methyl-beta-cyclodextrin reduced the punctate surface staining of TN-C. Consistently, TN-C was associated with lipid rafts of neonatal mouse brain according to sucrose density gradient centrifugation experiments. Furthermore, TN-R was also found in rafts prepared from myelin of adult mice. Thus, brain-derived tenascins are able to associate with lipid rafts.
authors.affiliation: Physiologisch-Chemisches Institut, Nussallee 11, 53115 Bonn, Germany. kappler@institut.physiochem.uni-bonn.de
authors.forename: Joachim
authors.initials: J
authors.lastname: Kappler
authors.forename: Stephan L
authors.initials: SL
authors.lastname: Baader
authors.forename: Sebastian
authors.initials: S
authors.lastname: Franken
authors.forename: Penka
authors.initials: P
authors.lastname: Pesheva
authors.forename: Karl
authors.initials: K
authors.lastname: Schilling
authors.forename: Uwe
authors.initials: U
authors.lastname: Rauch
authors.forename: Volkmar
authors.initials: V
authors.lastname: Gieselmann
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D021962:Membrane Microdomains / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D011247:Pregnancy; D019063:Tenascin / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; D019063:Tenascin; C074638:tenascin R