title: The large subunit of replication factor C interacts with the histone deacetylase, HDAC1.
abstract: Replication factor C (RFC) is a pentameric complex of five distinct subunits that functions as a clamp loader, facilitating the loading of proliferating cell nuclear antigen (PCNA) onto DNA during replication and repair. More recently the large subunit of RFC, RFC (p140), has been found to interact with the retinoblastoma (Rb) tumor suppressor and the CCAAT/enhancer-binding protein alpha (C/EBP alpha) transcription factor. We now report that RFC (p140) associates with histone deacetylase activity and interacts with histone deacetylase 1 (HDAC1). This complex is functional and when targeted to promoters as a Gal4 fusion, RFC (p140) is a strong, deacetylase-dependent repressor of transcription. Further analysis revealed that RFC (p140) contains two distinct transcriptional repression domains. Moreover, both of these domains interact separately with HDAC1.
authors.affiliation: Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Medical Sciences Institute/Wellcome Trust Biocentre Complex, Dow Street, Dundee DD1 5EH, United Kingdom.
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Anderson
authors.forename: Neil D
authors.initials: ND
authors.lastname: Perkins
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D051818:Replication Protein C
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases; D051818:Replication Protein C