title: Influence of resistant starch on the SCFA production and cell counts of butyrate-producing Eubacterium spp. in the human intestine.
abstract: OBJECTIVE
  The genus Eubacterium, which is the second most common genus in the human intestine, includes several known butyrate producers. We hypothesized that Eubacterium species play a role in the intestinal butyrate production and are inducible by resistant starch.
  
  
  RESULTS
  In a human pilot study species-specific and group-specific 16S rRNA-targeted, Cy3 (indocarbocyanine)-labelled oligonucleotide probes were used to quantify butyrogenic species of the genera Eubacterium, Clostridium and Ruminococcus. Following the intake of RS type III a significant increase in faecal butyrate but not in total SCFA was observed. However, increase in butyrate was not accompanied by a proliferation in the targeted bacteria.
  
  
  CONCLUSIONS
  The tested Eubacterium species have the capacity to produce butyrate but do not appear to play a major role for butyric acid production in the human intestine.
  
  
  CONCLUSIONS
  In view of the fact that the bacteria responsible for butyrate production are largely unknown, it is still difficult to devise a dietary intervention to stimulate butyrogenic bacteria in a targeted way.
authors.affiliation: Deutsches Institut für Ernährungsforschung, Abteilung Gastrointestinale Mikrobiologie, Germany. andy@www.dife.de
authors.forename: A
authors.initials: A
authors.lastname: Schwiertz
authors.forename: U
authors.initials: U
authors.lastname: Lehmann
authors.forename: G
authors.initials: G
authors.lastname: Jacobasch
authors.forename: M
authors.initials: M
authors.lastname: Blaut
mesh_terms: D000085:Acetates / Q000032:analysis / Q000378:metabolism; D000328:Adult; D002087:Butyrates / Q000032:analysis / Q000378:metabolism*; D015169:Colony Count, Microbial; D005051:Eubacterium / Q000235:genetics / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D005243:Feces / Q000737:chemistry; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011422:Propionates / Q000032:analysis / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012680:Sensitivity and Specificity; D013213:Starch / Q000008:administration & dosage* / Q000378:metabolism
chemical_list: D000085:Acetates; D002087:Butyrates; D005232:Fatty Acids, Volatile; D011422:Propionates; D012336:RNA, Ribosomal, 16S; D013213:Starch