Characterisation of Ag1, the major species-specific contaminant of bovine crude heparin, and its identification as an aprotinin/heparin complex.
Heparin is a potent anticoagulant polysaccharide purified for decades from ruminants or porcine tissues. However, with the emergence of bovine spongiform encephalopathy (BSE), the source of pharmaceutical heparin is currently restricted to porcine intestinal mucosa. A major species-specific contaminant, called Ag1, has recently been identified in bovine crude heparin [Rivera et al., J. Pharm. Biomed. Anal., submitted] and used to develop an enzyme-linked immunosorbent assay (ELISA) for the species origin control of crude heparins [Levieux et al., J. Immunoassay, submitted]. In this report, we describe the different investigations, which were carried out to identify Ag1. This antigen was first localised by immunohistological studies essentially in the connective tissue of the bovine small intestine. After extraction from an intestinal extract by immuno-affinity chromatography, Ag1 was isolated as a single band by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Ag1 was then partly sequenced and identified as an aprotinin/heparin complex. Aprotinin, also known as the bovine pancreatic trypsin inhibitor (BPTI), is present with heparin in mast cells, and is very resistant to heat, pH, chemical treatments and proteolytic digestion. The stability of Ag1 towards the different treatments performed during heparin extraction process allows this protein to remain in sufficient amounts in crude heparin and makes it an ideal target for the immunochemical control of the absence of bovine material in crude heparins.
Rivera
Vincent
V
INRA, Station de Recherches sur la Viande, Immunochimie, Theix, 63122 Saint-Genès-Champanelle, France.
Levieux
Annie
A
Levieux
Didier
D
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D007611:Aprotinin / Q000276:immunology* / Q000302:isolation & purification*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D004340:Drug Contamination; D004591:Electrophoresis, Polyacrylamide Gel; D006493:Heparin / Q000032:analysis* / Q000276:immunology*; D007106:Immune Sera / Q000096:biosynthesis; D015151:Immunoblotting; D007421:Intestine, Small / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D011817:Rabbits; D013045:Species Specificity
D000906:Antibodies; D007106:Immune Sera; D006493:Heparin; D007611:Aprotinin