title: Zinc is required for structural stability of the C-terminus of archaeal translation initiation factor aIF2beta.
abstract: aIF2beta is an archaeal homolog of eukaryotic translation factor eIF2beta necessary for translation initiation and involved in recognition of the initiation codon. In the present study, we demonstrate for the first time zinc binding to the C2-C2 zinc finger at the C-terminus of aIF2beta. Nuclear magnetic resonance backbone assignments were also determined and the secondary structural elements identified.
authors.affiliation: McGill University, Department of Biochemistry, McIntyre Medical Science Building, 3655 Promenade Sir William Osler, Montréal, QC, Canada.
authors.forename: Pablo
authors.initials: P
authors.lastname: Gutiérrez
authors.forename: Stéphane
authors.initials: S
authors.lastname: Coillet-Matillon
authors.forename: Cheryl
authors.initials: C
authors.lastname: Arrowsmith
authors.forename: Kalle
authors.initials: K
authors.lastname: Gehring
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry / Q000378:metabolism*; D019605:Euryarchaeota / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis / Q000502:physiology; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D015032:Zinc / Q000378:metabolism* / Q000502:physiology; D016335:Zinc Fingers / Q000502:physiology*
chemical_list: D019843:Archaeal Proteins; D015032:Zinc