blaCTX-M-2 is located in an unusual class 1 integron (In35) which includes Orf513.
Examination of the bla(CTX-M-2) gene in plasmid pMAR-12 by sequencing and PCR analysis revealed that the bla gene and the surrounding DNA, which is closely related (99% homology) to the Kluyvera ascorbata chromosomal DNA that contains the bla(KLUA-1) gene, are located in a complex sul1-type integron, termed In35, that includes Orf513. It is possible that bla(CTX-M-2) was acquired by plasmid pMAR-12 through an uncharacterized recombinational event in which Orf513 could be involved.
Arduino
Sonia M
SM
Facultad de Medicina de la Universidad de Buenos Aires, Paraguay 2155 (1121)Capital Federal, Argentina. sarduino@aol.com
Roy
Paul H
PH
Jacoby
George A
GA
Orman
Betina E
BE
Pineiro
Silvia A
SA
Centron
Daniela
D
D001483:Base Sequence; D005798:Genes, Bacterial; D040342:Genetic Structures; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
C071167:beta-lactamase CTX-2; D001618:beta-Lactamases