Ubiquitin genes in rainbow trout (Oncorhynchus mykiss).
Ubiquitin is a small protein involved in intracellular proteolysis. It is highly conserved throughout eukaryotic phyla and has been detected in such diverse species as yeast, barley, Drosophila and man. A previous study showed that chromatin of rainbow trout testis contains free ubiquitin with a sequence similar to that of other phyla. In the present study, which focused on rainbow trout but included eleven other species, it is shown that fish ubiquitin genetic organisation and expression are similar to those of other phylogenetic groups through the following set of observations: (a) Multiple loci were detected, (b) These loci encode repeats of ubiquitin, (c) Although the DNA sequences are not conserved, the encoded amino acid sequences are fully conserved, (d) The expression of ubiquitin was influenced by cell culture conditions and viral infection.
Okubo
Kazue
K
Department of Aquatic Biosciences, Tokyo University of Fisheries, Japan.
Yamano
Keisuke
K
Qin
Qiwei
Q
Aoyagi
Kazuhiko
K
Ototake
Mitsuru
M
Nakanishi
Teruyuki
T
Fukuda
Hideo
H
Dijkstra
Johannes M
JM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry; D015870:Gene Expression; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012313:RNA / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017421:Sequence Analysis; D017385:Sequence Homology; D013045:Species Specificity; D025801:Ubiquitin / Q000737:chemistry / Q000235:genetics*
D025801:Ubiquitin; D012313:RNA; D004247:DNA