Reduced inositol content in lymphocyte-derived cell lines from bipolar patients.
OBJECTIVE
The study aimed to determine whether low inositol content and uptake previously reported in brain and peripheral tissue of bipolar patients are also reflected in lymphocyte-derived cell lines from these patients.


METHODS
Inositol content and uptake were studied in lymphocyte-derived cell lines grown in vitro for at least five generations to eliminate influences of drug treatment. Inositol content was studied gas chromatographically and inositol uptake by following 3H-inositol incorporation at various concentrations.


RESULTS
Inositol levels of cell lines derived from bipolar patients were significantly lower than those of cell lines from controls.


CONCLUSIONS
Low inositol content in lymphocyte-derived cell lines from bipolar patients corroborates previous findings in frontal cortex and in lymphoblastoid cell lines and are consistent with the notion that the phosphatidylinositol signaling system is involved in the pathophysiology of this disorder.
Belmaker
R H
RH
Stanley Foundation Research Center, Ben-Gurion University of the Negev, Beersheva, Israel.
Shapiro
J
J
Vainer
E
E
Nemanov
L
L
Ebstein
R P
RP
Agam
G
G
D001714:Bipolar Disorder / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D002849:Chromatography, Gas; D005260:Female; D005625:Frontal Lobe / Q000378:metabolism; D006801:Humans; D007294:Inositol / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D007294:Inositol