title: Absence of mutations in the transforming growth factor-beta inducible early gene 1, TIEG1, in pancreatic cancer.
abstract: Pancreatic cancers frequently have defects in components of the transforming growth factor-beta (TGF-beta) signaling pathway. TIEG1 (TGF-beta inducible early gene) is a recently characterized transcription factor regulated by TGF-beta that induces apoptosis when overexpressed in pancreatic adenocarcinoma cell lines. Alterations on chromosome 8q, where TIEG1 is located, are also relatively frequent in pancreatic cancers. To determine if TIEG1 may be involved in the tumorigenesis of pancreatic cancer, we performed mutational screening of this gene in 22 pancreatic cancer cell lines. No sequence alterations were observed. Reverse transcription-polymerase chain reaction analysis was also performed to rule out the possibility that the expression of the gene is altered by genetic events other than mutation. Likewise, no alterations in expression were found. Thus, an essential role of TIEG1 in pancreatic cancer can be excluded.
authors.affiliation: Department of Pathology, Università di Verona, Strada Le Grazie 8, 37134, Verona, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Antonello
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Moore
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Zamboni
authors.forename: Massimo
authors.initials: M
authors.lastname: Falconi
authors.forename: Aldo
authors.initials: A
authors.lastname: Scarpa
mesh_terms: D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics*; D051765:Early Growth Response Transcription Factors; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D009154:Mutation; D010190:Pancreatic Neoplasms / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; D051765:Early Growth Response Transcription Factors; C098111:KLF10 protein, human; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors