Development of protein-detecting microarrays and related devices.
There is great interest in the development of devices capable of monitoring the levels and post-translational modification states of hundreds or thousands of proteins simultaneously. One way to do this would be to create protein-detecting microarrays roughly akin to the DNA microarrays that are used for genome-wide expression studies. Two major challenges must be addressed before practical devices of this type become available. One is the development of high-throughput methods for the isolation of protein-binding compounds that will act as capture molecules in the array. The second is the optimization of methods that register binding of target proteins to the immobilized ligands in a sensitive and quantitative fashion. Progress in these areas, and some of the challenges remaining, are reviewed in this article.
Kodadek
Thomas
T
Dept of Internal Medicine, Center for Biomedical Inventions, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-8573, USA. thomas.kodadek@utsouthwestern.edu
D000906:Antibodies; D004867:Equipment Design; D020869:Gene Expression Profiling / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008024:Ligands; D020411:Oligonucleotide Array Sequence Analysis; D010449:Peptide Mapping / Q000295:instrumentation* / Q000379:methods; D011485:Protein Binding; D011499:Protein Processing, Post-Translational
D000906:Antibodies; D008024:Ligands