Oxidative and cold shock cause enhanced induction of a 50 kDa stress protein in Trichinella spiralis.
The anti-heat shock protein (Hsp)-90 monoclonal antibody AC-16 reacts on blots with Hsp90 and a 50 kDa protein (prot-50) from infective-stage (L1) larvae of the nematode Trichinella spiralis. We examined Hsp90 and prot-50 levels by densitometric analysis of immunoblots of T. spiralis larval extracts prepared before (time 0, 37 degrees C) and after oxidative [hydrogen peroxide (H2O2)] stress, or cold shock at 4 degrees C. Extracts from H2O2-exposed L1 were obtained after 2 h; the others at 2, 4, and 8 h after the temperature shift. After H2O2 shock, the constitutive Hsp90 and prot-50 were both significantly induced and appeared as slower migrating inducible isoforms. However, whereas Hsp90 levels decreased after cold shock, prot-50 levels immediately and persistently increased after shock at 4 degrees C. These data present compelling evidence that the prot-50 described here functions as a Hsp and a cold shock protein.
Martinez
J
J
Facultad de Farmacia, Departamento de Microbiologia y Parasitologia, Universidad de Alcala, Madrid, Spain. francisco.martinez@uah.es
Perez-Serrano
J
J
Bernadina
W E
WE
Rodriguez-Caabeiro
F
F
D000818:Animals; D015153:Blotting, Western; D003080:Cold Temperature; D005786:Gene Expression Regulation; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D018869:Heat-Shock Response; D015801:Helminth Proteins / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress; D017160:Trichinella spiralis / Q000187:drug effects / Q000254:growth & development*
D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D015801:Helminth Proteins; D006861:Hydrogen Peroxide