Pathogenesis of infections with Salmonella enterica subsp. enterica serovar Muenchen in the turtle Trachemys scripta scripta.
The pathogenesis of Salmonella enterica subsp. enterica serovar Muenchen infections in the aquatic turtle Trachemys scripta scripta was studied. After oral infection with 5x10(5)cfu of serovar Muenchen of 10-14-month-old turtles, kept at 26 degrees C, the intestine and especially the ileum, caecum and colon was colonized. Invasion of the intestinal wall, causing histopathological lesions, and colonization of internal organs were not observed. Serovar Muenchen was only isolated from turtles for 8 days after exposure. Keeping the turtles at 37 degrees C caused colonization of liver and spleen in two of six orally infected turtles and augmented the numbers of bacteria in the intestinal tract. In contrast to oral infections, intraperitoneal infections of turtles with serovar Muenchen enabled the bacterium to persist inside the host for at least 5 weeks. Clearance of serovar Muenchen from the liver and blood was more pronounced at 26 degrees C than at 37 degrees C. ELISA antibodies were demonstrated in intraperitoneally but not in orally infected turtles kept at 26 degrees C. In conclusion, the lack of persistence and invasiveness of serovar Muenchen in T. s. scripta after oral exposure might be due to the turtle's relatively low body temperature and/or the absence of well-organized gut-associated lymphoid tissue.
Pasmans
Frank
F
Faculty of Veterinary Medicine, Department of Pathology, Bacteriology and Avian Diseases, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. frank.pasmans@rug.ac.be
De Herdt
Peter
P
Dewulf
Jeroen
J
Haesebrouck
Freddy
F
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001831:Body Temperature / Q000502:physiology*; D015169:Colony Count, Microbial / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007422:Intestines / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D019779:Salmonella enterica / Q000254:growth & development*; D014426:Turtles / Q000382:microbiology*
D000907:Antibodies, Bacterial