title: Characterization of the chondroitin sulfates in wild type Caenorhabditis elegans.
abstract: The purpose of this study was to isolate and characterize the GAGs from the wild type nematode Caenorhabditis elegans in preparation for the characterization of the transgenic form constructed by Link [Proc. Natl. Acad. Sci. USA 92 (1995) 9368] which expresses various forms of beta-peptide (or A4 peptide). This peptide forms deposits very similar to the ones found in the neuritic plaques and neurofibrillary tangles in Alzheimer disease (AD). Characterization has been accomplished by degradation with specific enzymes and analysis of the products by TLC and HPLC. The results were compared with earlier works and shown to differ in disaccharide content.
authors.affiliation: Department of Biological Sciences, Kingsborough Community College/CUNY, 2001 Oriental Blvd., Brooklyn, NY 11235, USA. cbeeber@kbcc.cuny.edu
authors.forename: Carla
authors.initials: C
authors.lastname: Beeber
authors.forename: Fred J
authors.initials: FJ
authors.lastname: Kieras
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000737:chemistry; D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology*; D002809:Chondroitin Sulfates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry; D010455:Peptides / Q000737:chemistry
chemical_list: D016564:Amyloid beta-Protein Precursor; D006025:Glycosaminoglycans; D010455:Peptides; D002809:Chondroitin Sulfates