title: Human proteinase 3 can inhibits LPS-mediated TNF-alpha production through CD14 degradation: lack of influence of antineutrophil cytoplasmic antibodies.
abstract: The present study was conducted to investigate if proteinase-3 (PR3) is able to influence lipopolysaccharide (LPS) responses of monocytes via degradation of CD14 and if antineutrophil cytoplasmic antibodies (ANCA) may modify this process. Recombinant (r) CD14 and CD14 expressed on monocytes were investigated for PR3 mediated degradation by SDS-PAGE and FACS analysis, respectively. TNF-alpha production in whole blood was used to determine functional consequences of CD14 degradation. PR3 degraded rCD14 in a dose- and time-dependent fashion. Major degradation products were found with apparent molecular weight of 45, 25 and 10 kDa. Treatment of PR3 with PMSF completely abolished CD14 degradation. ANCA IgG did not inhibit CD14 degradation. In whole blood, addition of PR3 resulted in diminished CD14 expression on monocytes. In contrast, CD14 was increased in a subpopulation of cells that expressed major histocompatibility (MHC) class II and PR3, but lacked expression of CD64 and CD16. LPS mediated TNF-alpha production in whole blood was significantly inhibited when preincubated with PR3. This study demonstrates that PR3 can degrade rCD14 and that PR3 differentially affects CD14 expression in subsets of monocytes. ANCA IgG does not play a significant role herein.
authors.affiliation: Medizinische Universitätsklinik, Klinikum Mannheim, University of Heidelberg, Germany. benito.yard@med5.ma.uni-heidelberg.de
authors.forename: B A
authors.initials: BA
authors.lastname: Yard
authors.forename: A-I
authors.initials: AI
authors.lastname: Wille
authors.forename: M
authors.initials: M
authors.lastname: Haak
authors.forename: F J
authors.initials: FJ
authors.lastname: van der Woude
mesh_terms: D019268:Antibodies, Antineutrophil Cytoplasmic / Q000378:metabolism* / Q000494:pharmacology; D001324:Autoantigens / Q000378:metabolism*; D002478:Cells, Cultured; D014890:Granulomatosis with Polyangiitis / Q000378:metabolism*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D053146:Myeloblastin; D012697:Serine Endopeptidases / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D001324:Autoantigens; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D012697:Serine Endopeptidases; D053146:Myeloblastin