title: Identification of Escherichia coli O157:H7-strains from pigs with postweaning diarrhoea and amplification of their virulence marker genes by PCR.
abstract: Escherichia coli isolated from pigs with postweaning diarrhoea were examined by PCR for the presence of the O157 rfb gene responsible for the biosynthesis of E coli O157 lipopolysaccharide. Among the 372 isolates tested, 38 (10.2 per cent) were of the O157 serogroup, but none of these possessed the H7 determinant. Further analysis of the E coli O157 isolates revealed that seven of them had the genes responsible for the production of Shiga toxin 1 and eaeA intimin, four other strains had genes responsible for the production of Shiga toxin 2, and four other strains were positive for the enterohaemolysin gene.
authors.affiliation: Department of Microbiology, National Veterinary Research Institute, Pulawy, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Osek
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D022621:Shiga Toxin / Q000235:genetics; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D018829:Adhesins, Bacterial; D002352:Carrier Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D037521:Virulence Factors; C073819:eaeA protein, E coli; D022621:Shiga Toxin