Morphological changes during conoid extrusion in Toxoplasma gondii tachyzoites treated with calcium ionophore.
Treatment of tachyzoites of Toxoplasma gondii with the calcium ionophore A23187 induced dramatic ultrastructural changes that were observed by light and electron microscopy. Light microscopy showed a higher percentage (22%) of tachyzoites with the conoid extruded when compared to control parasites. Electron microscopy confirmed the conoid extrusion by both transmission and scanning electron microscopy. Freeze-fracture replicas showed that the plasma membrane adjacent to cytoplasmic dense granules appeared devoid of intramembranous particles. Membrane-limited vesicles and filopodium-like structures at the cell surface were observed in treated cells. 3-D reconstruction from serial sections confirmed the data and showed a heterogeneity in dense granule shape not reported in control cells.
Monteiro
V G
VG
Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego 2000, Campos, RJ, Brazil.
de Melo
E J
EJ
Attias
M
M
de Souza
W
W
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D002462:Cell Membrane / Q000648:ultrastructure; D005614:Freeze Fracturing; D007091:Image Processing, Computer-Assisted; D007425:Intracellular Membranes / Q000648:ultrastructure; D007476:Ionophores / Q000494:pharmacology; D008854:Microscopy, Electron; D014122:Toxoplasma / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*
D007476:Ionophores; D000001:Calcimycin