The APC regulator CDH1 is essential for the progression of embryonic cell cycles in Xenopus.
The orderly progression of cell cycle depends on timely destruction of key regulators through ubiquitin-mediated proteolysis. The anaphase-promoting complex (APC) is a major component of this degradation machinery and its activation is regulated by CDC20 and CDH1. We demonstrate here that CDH1 mRNA is ubiquitously expressed in Xenopus embryos of all developmental stages. Loss of CDH1 function during early embryonic cell cycles leads to an immediate and prolonged arrest with low cyclin-dependent kinase activity. In contrast, ectopic overexpression of CDH1 induces cell cycle arrest during the first G(1) phase at the midblastula transition. CDH1-dependent degradation of cyclin A is likely involved in this G(1) arrest. Our findings establish the essential roles of CDH1 in embryonic cell cycles.
Zhou
Yuan
Y
Institute of Molecular Biology, The University of Hong Kong, Hong Kong, China.
Ching
Yick-Pang
YP
Ng
Raymond W M
RW
Jin
Dong-Yan
DY
D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D064199:Cdc20 Proteins; D064200:Cdh1 Proteins; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins; D019925:Cyclin A / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D016193:G1 Phase; D008025:Ligases / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D014982:Xenopus laevis / Q000196:embryology*
C577495:CDC20 protein, S cerevisiae; C577583:CDH1 protein, S cerevisiae; D064199:Cdc20 Proteins; D064200:Cdh1 Proteins; D018797:Cell Cycle Proteins; D019925:Cyclin A; D005656:Fungal Proteins; D011506:Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D008025:Ligases