Epstein-Barr virus-positive Hodgkin/Reed-Sternberg-like B cell in non-hodgkin lymphoma: nucleotide sequence of the amplified immunoglobulin heavy-chain variable region gene by the single-cell polymerase chain reaction technique.
We examined nucleotide sequences of Epstein-Barr virus (EBV)-positive Hodgkin/Reed-Sternberg (HRS)-like B cells in a case of diffuse large B-cell lymphoma (DLBCL) and a case of adult T-cell lymphoma (ATL) for single-cell polymerase chain reaction of the immunoglobulin heavy-chain gene variable region (VH gene). HRS-like B cells were scattered in the area irrelevant to the lymphoma infiltrates of DLBCL and in the lymphoma area of ATL. HRS-like B cells were positive for CD20 and CD30 but negative for CD15. EBV presented in HRS-like B cells in both cases but not in any lymphoma cells. VH genes of five HRS-like B cells analyzed in DLBCL were polyclonal and showed in-frame sequences with 0% to 2.8% somatic mutation frequency. In an ATL, VH genes of five HRS-like B cells analyzed were polyclonal and somatically mutated. Four cells carried in-frame rearrangements with 3.5% to 17.7% mutation frequency. One of the VH genes has a one-codon deletion. From the fifth cell, an out-of-frame rearrangement with an insertion and a deletion was obtained. Thus, we showed polyclonal EBV-positive HRS-like B cells in both DLBCL and ATL and that whereas EBV-positive, HRS-like B cells in DLBCL exhibited unmutated and mutated VH gene, those in ATL were found to have a somatically mutated VH gene with/without deletions and/or insertions. The HRS-like B cells may appear because of active EBV infection in a patient who is immunosuppressed from the primary lymphoma.
Nakamura
Naoya
N
Department of Pathology, Fukushima Medical University School of Medicine, Fukushima-shi, Japan. nao@cc.fmu.ac.jp
Mori
Naoyoshi
N
Ohshima
Koichi
K
Kuze
Tetsuo
T
Hashimoto
Yuko
Y
Sasaki
Yoshikazu
Y
Shirakawa
Asumi
A
Sato
Michiko
M
Abe
Masafumi
M
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology* / Q000821:virology; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000032:analysis; D004210:Dissection; D020031:Epstein-Barr Virus Infections / Q000473:pathology*; D005803:Genes, Immunoglobulin; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D016130:Immunophenotyping; D017403:In Situ Hybridization; D008198:Lymph Nodes / Q000473:pathology / Q000821:virology; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000473:pathology* / Q000821:virology; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000473:pathology / Q000821:virology; D008297:Male; D008846:Micromanipulation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D016539:Reed-Sternberg Cells / Q000473:pathology* / Q000821:virology
D015703:Antigens, CD; D004273:DNA, Neoplasm; C099537:Epstein-Barr virus encoded RNA 1; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D012367:RNA, Viral