Association between the strength of serologic recognition of bovine leukosis virus and lymphocyte count in bovine leukosis virus-infected cows.
OBJECTIVE
To determine whether strength of serologic recognition of bovine leukosis virus (BLV) by use of ELISA is associated with blood lymphocyte counts.


METHODS
Prospective study.


METHODS
161 cows with positive results of ELISA for BLV.


METHODS
Sample-to-positive ratio (S:P), which is the ratio between the test sample and a positive control sample, was compared among lymphocytotic and nonlymphocytotic cows. A regression model was constructed to evaluate the association between blood lymphocyte concentration and S:P, age, and the interaction of these terms.


RESULTS
Mean S:P differed significantly between lymphocytotic (2.58 +/- 0.36) and nonlymphocytotic (2.38 +/- 0.39) cows. Age and S:P were significantly associated with lymphocyte count.


CONCLUSIONS
Sample-to-positive ratio and lymphocyte count were related; however, cows with high S:P were not always lymphocytotic. Culling cows on the basis of S:P will reduce the herd load of infectious virus faster than random culling of ELISA-positive cows; however, culling on the basis of lymphocyte count will eliminate a greater proportion of the reservoir of infection.
Nagy
Dusty W
DW
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana 61802, USA.
Tyler
Jeff W
JW
Stoker
Aaron
A
Kleiboeker
Steven B
SB
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D018655:Lymphocyte Count / Q000662:veterinary; D008218:Lymphocytosis / Q000175:diagnosis / Q000662:veterinary*; D011446:Prospective Studies
D000914:Antibodies, Viral