ARHGAP10, a novel human gene coding for a potentially cytoskeletal Rho-GTPase activating protein.
Rho-GTPase activating proteins (Rho-GAPs) are negative regulators of Rho-GTPase signaling pathways related to actin cytoskeleton dynamics, cell proliferation, and differentiation. We have identified a novel human gene, termed ARHGAP10, that codes for a 1957-aminoacid Rho-GAP, containing a PDZ, a PH, and a Rho-GAP domain. The cDNA is 7118 bp long and has an open reading frame of 5874 bp. A computational analysis located this gene on chromosome 10 band 10p12.32 suggesting that it is composed of 25 exons. Northern analysis revealed that it is widely expressed, with high levels in brain and muscle. Real-time quantitative PCR analysis confirmed an increase in ARHGAP10 expression during differentiation of HL-60 cells with all-trans-retinoic acid and hematopoietic stem cells with erythropoietin, suggesting that this gene could play a role in normal hematopoiesis. The fact that this gene is highly expressed in muscle and brain, which are highly differentiated tissues, further supports the hypothesis that ARHGAP10 is important for cell differentiation.
Bassères
Daniela Sanchez
DS
Centro de Hematologia e Hemoterapia, Universidade Estadual de Campinas, Campinas, SP 13083-970, Brazil. sara@unicamp.br
Tizzei
Edna Vedelago
EV
Duarte
Adriana A S
AA
Costa
Fernando Ferreira
FF
Saad
Sara Teresinha Olalla
ST
D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D020690:GTPase-Activating Proteins / Q000096:biosynthesis / Q000235:genetics*; D015894:Genome, Human; D018922:HL-60 Cells; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D009928:Organ Specificity; D020742:rhoA GTP-Binding Protein
C486050:ARHGAP10 protein, human; C459590:ARHGAP21 protein, human; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D020690:GTPase-Activating Proteins; D020742:rhoA GTP-Binding Protein