[Biochemical and ultrastructural characterization of the effect of interleukin 1-beta as mediator of the degradation of connective tissue in human chorioamniotic membranes in culture].
OBJECTIVE
To characterize the effect of IL-1 beta, on connective tissue metabolism in a human chorioamniotic membrane tissue culture (CAM).


METHODS
Experimental, in an in vitro model.


METHODS
CAM explants obtained from cesarean sections were cultured. The presence of local infection was excluded by microbiological methods. An XTT viability essay of the explants was carried out. Explants were stimulated with different doses of IL-1 beta within a 0-10 ng/mL range. After the stimulation, protein content was measured, MMP-9 production was determined by zymography, and each explant was divided in two parts: one was used for collagen measurement and the other analyzed by electronic microscopy.


RESULTS
CAMs kept adequate viability and functionality. IL-1 beta stimulation produced an increase in the amount of MMP-9 expressed, as determined by the zymography method with a maximum effect 36 hours after stimulation. Collagen content decreased in a progressive manner after IL-1 beta stimulation and reached its minimum after 36 hours. The characteristic pattern of collagen fibers gradually lost its organization, and could not be observed any more after 36 hours.


CONCLUSIONS
The information presented here allows us to conclude that IL-1 beta is capable of inducing an enzymatic expression affecting connective tissue, thus confirming its participation in membrane degradation processes under inflammatory conditions.
Estrada Gutiérrez
Guadalupe del Carmen
Gdel C
Departamento de Microscopia Electrónica, Escuela Nacional de Ciencias Biológicas, I.P.N.
Vadillo-Ortega
Felipe
F
Jiménez González
Marco A
MA
Beltrán Montoya
Jorge
J
Giono Cerezo
Silvia
S
D000650:Amnion / Q000187:drug effects* / Q000378:metabolism; D002823:Chorion / Q000187:drug effects* / Q000378:metabolism; D003094:Collagen / Q000187:drug effects* / Q000378:metabolism; D003238:Connective Tissue / Q000187:drug effects* / Q000378:metabolism; D046508:Culture Techniques; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*
D007375:Interleukin-1; D003094:Collagen; D020780:Matrix Metalloproteinase 9
Caracterización bioquímica y ultraestructural del efecto de la interleucina 1-beta como mediador de degradación de tejido conectivo en membranas corioamnióticas humanas en cultivo.