Expression of cytosolic and group X secretory phospholipase A(2) genes in human colorectal adenocarcinomas.
Gene expression of cytosolic phospholipase A(2) (cPLA(2)) and protein level of secretory PLA(2) group X (sPLA(2)-X) are upregulated in human colorectal cancer and provide cyclooxygenase-2 (COX-2) with arachidonic acid, resulting in increased levels of PGE(2). Mutated ras-genes are suggested to be involved in the regulatory pathway of cPLA(2) in lung cancer cells. We analysed the gene expression of cPLA(2) and sPLA(2)-X in 42 and 38 primary colorectal tumours, respectively, with and without K-ras mutations. We found an up-regulation of cPLA(2) mRNA but the induction in tumour tissues does not correlate with Ras-gene mutations. Moreover, our results cannot consistently reflect an overexpression of sPLA(2)-X gene in colorectal cancer tissues.
Osterström
Anna
A
Department of Biomedicine and Surgery, Division of Cell Biology, Faculty of Health Sciences, Linköping, Sweden. anna.osterstrom@hhj.hj.se
Dimberg
Jan
J
Fransén
Karin
K
Söderkvist
Peter
P
D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics* / Q000601:surgery; D001483:Base Sequence; D003110:Colonic Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000601:surgery; D017931:DNA Primers; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D054513:Group IV Phospholipases A2; D054509:Group X Phospholipases A2; D006801:Humans; D010741:Phospholipases A / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D012333:RNA, Messenger; D010741:Phospholipases A; D054513:Group IV Phospholipases A2; D054509:Group X Phospholipases A2; C516365:PLA2G10 protein, human; C516389:PLA2G4A protein, human