Manganese supplementation relieves the phenotypic deficits seen in superoxide-dismutase-null Escherichia coli.
Escherichia coli, lacking cytoplasmic superoxide dismutases, exhibits a variety of oxygen-dependent phenotypic deficits. Enrichment of the growth medium with Mn(II) relieved those deficits. Extracts of cells grown on Mn(II)-rich medium exhibited superoxide dismutase-like activity that was due partially to low-molecular-weight and partially to high-molecular-weight complexes. The high-molecular-weight activity was sensitive to proteolysis. Hence this activity is likely associated with low-affinity binding of Mn to proteins.
Al-Maghrebi
May
M
Department of Biochemistry, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait.
Fridovich
Irwin
I
Benov
Ludmil
L
D000154:Aconitate Hydratase / Q000378:metabolism; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008345:Manganese / Q000008:administration & dosage / Q000494:pharmacology*; D008970:Molecular Weight; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000502:physiology*
D003470:Culture Media; D017382:Reactive Oxygen Species; D008345:Manganese; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D000154:Aconitate Hydratase