Identification of genes periodically expressed in the human cell cycle and their expression in tumors.
The genome-wide program of gene expression during the cell division cycle in a human cancer cell line (HeLa) was characterized using cDNA microarrays. Transcripts of >850 genes showed periodic variation during the cell cycle. Hierarchical clustering of the expression patterns revealed coexpressed groups of previously well-characterized genes involved in essential cell cycle processes such as DNA replication, chromosome segregation, and cell adhesion along with genes of uncharacterized function. Most of the genes whose expression had previously been reported to correlate with the proliferative state of tumors were found herein also to be periodically expressed during the HeLa cell cycle. However, some of the genes periodically expressed in the HeLa cell cycle do not have a consistent correlation with tumor proliferation. Cell cycle-regulated transcripts of genes involved in fundamental processes such as DNA replication and chromosome segregation seem to be more highly expressed in proliferative tumors simply because they contain more cycling cells. The data in this report provide a comprehensive catalog of cell cycle regulated genes that can serve as a starting point for functional discovery. The full dataset is available at http://genome-www.stanford.edu/Human-CellCycle/HeLa/.
Whitfield
Michael L
ML
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA.
Sherlock
Gavin
G
Saldanha
Alok J
AJ
Murray
John I
JI
Ball
Catherine A
CA
Alexander
Karen E
KE
Matese
John C
JC
Perou
Charles M
CM
Hurt
Myra M
MM
Brown
Patrick O
PO
Botstein
David
D
D002453:Cell Cycle / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D004798:Enzymes / Q000235:genetics; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D014644:Genetic Variation; D015894:Genome, Human; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D005810:Multigene Family; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D004798:Enzymes; D011506:Proteins