title: Effect of extracellular matrix proteins on platelet-activating factor-induced eosinophil chemotaxis.
abstract: BACKGROUND
  Eosinophils accumulate in tissues during various allergic inflammatory processes. Selective eosinophil recruitment is the result of orchestrated events involving cell adhesion molecules and chemoattractants. However, the exact mechanism of the regulation of integrin avidity during interstitial migration is poorly understood.
  
  
  OBJECTIVE
  The purpose of this study was to investigate whether extracellular matrix proteins might activate human eosinophil chemotaxis and, if so, to clarify the mechanism in terms of integrin avidity.
  
  
  METHODS
  Eosinophils were purified from the peripheral blood of healthy donors. Eosinophil migration was measured using Boyden chambers with filter membranes coated with fibronectin (Fn), vitronectin (Vn), laminin (Ln), hyaluronic acid, collagen type IV, or bovine serum albumin (BSA) overnight. Platelet-activating factor (PAF) was introduced into the lower chamber of each well. Eosinophils were placed in the upper chamber after incubation with IL-5 for 15 min. The number of eosinophils that transmigrated into the lower chamber was calculated by measuring the eosinophil peroxidase activity.
  
  
  RESULTS
  Fn, Ln and BSA enhanced PAF-induced chemotaxis of eosinophils. Inhibition experiments using blocking monoclonal antibodies showed that in the early phase of chemotaxis, Fn and Ln facilitated eosinophil chemotaxis that was mediated by alpha4 and alpha6 integrins, respectively. In the late phase of chemotaxis, BSA, but not other matrix proteins, facilitated both chemokinesis and chemotaxis that was mediated by beta2 integrin.
  
  
  CONCLUSIONS
  Our data strongly suggest that during chemotaxis, matrix proteins might activate eosinophils via binding with integrins to facilitate PAF-induced chemotaxis, and that such a mechanism might participate in allergic inflammatory processes.
authors.affiliation: Department of Allergy and Immunology, National Center for Child Health and Development Research Institute, Tokyo, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Iida
authors.forename: Akira
authors.initials: A
authors.lastname: Akasawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Moriyama
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Saito
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018821:CD18 Antigens / Q000502:physiology; D002417:Cattle; D002634:Chemotaxis, Leukocyte / Q000187:drug effects* / Q000502:physiology*; D024141:Collagen Type IV / Q000494:pharmacology; D004804:Eosinophils / Q000187:drug effects* / Q000276:immunology*; D016326:Extracellular Matrix Proteins / Q000494:pharmacology* / Q000502:physiology; D005353:Fibronectins / Q000494:pharmacology; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology; D006967:Hypersensitivity / Q000209:etiology / Q000276:immunology; D066298:In Vitro Techniques; D007249:Inflammation / Q000209:etiology / Q000276:immunology; D007797:Laminin / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D019096:Vitronectin / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D010972:Platelet Activating Factor; D019096:Vitronectin; D012710:Serum Albumin, Bovine; D006820:Hyaluronic Acid