title: Genetic linkage analysis of the lesser grain borer Rhyzopertha dominica identifies two loci that confer high-level resistance to the fumigant phosphine.
abstract: High levels of inheritable resistance to phosphine in Rhyzopertha dominica have recently been detected in Australia and in an effort to isolate the genes responsible for resistance we have used random amplified DNA fingerprinting (RAF) to produce a genetic linkage map of R. dominica. The map consists of 94 dominant DNA markers with an average distance between markers of 4.6 cM and defines nine linkage groups with a total recombination distance of 390.1 cM. We have identified two loci that are responsible for high-level resistance. One provides approximately 50x resistance to phosphine while the other provides 12.5x resistance and in combination, the two genes act synergistically to provide a resistance level 250x greater than that of fully susceptible beetles. The haploid genome size has been determined to be 4.76 x 10(8) bp, resulting in an average physical distance of 1.2 Mbp per map unit. No recombination has been observed between either of the two resistance loci and their adjacent DNA markers in a population of 44 fully resistant F5 individuals, which indicates that the genes are likely to reside within 0.91 cM (1.1 Mbp) of the DNA markers.
authors.affiliation: Department of Biochemistry, University of Queensland, St. Lucia, Queensland, Australia, 4072.
authors.forename: David I
authors.initials: DI
authors.lastname: Schlipalius
authors.forename: Qiang
authors.initials: Q
authors.lastname: Cheng
authors.forename: Paul E B
authors.initials: PE
authors.lastname: Reilly
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Collins
authors.forename: Paul R
authors.initials: PR
authors.lastname: Ebert
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D001517:Coleoptera / Q000235:genetics*; D003433:Crosses, Genetic; D016172:DNA Fingerprinting; D005434:Flow Cytometry; D005819:Genetic Markers; D016678:Genome; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides; D010720:Phosphines; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D005819:Genetic Markers; D007306:Insecticides; D010720:Phosphines; C044646:phosphine