ER-golgi traffic is a prerequisite for efficient ER degradation.
Protein quality control is an essential function of the endoplasmic reticulum. Misfolded proteins unable to acquire their native conformation are retained in the endoplasmic reticulum, retro-translocated back into the cytosol, and degraded via the ubiquitin-proteasome system. We show that efficient degradation of soluble malfolded proteins in yeast requires a fully competent early secretory pathway. Mutations in proteins essential for ER-Golgi protein traffic severely inhibit ER degradation of the model substrate CPY*. We found ER localization of CPY* in WT cells, but no other specific organelle for ER degradation could be identified by electron microscopy studies. Because CPY* is degraded in COPI coat mutants, only a minor fraction of CPY* or of a proteinaceous factor required for degradation seems to enter the recycling pathway between ER and Golgi. Therefore, we propose that the disorganized structure of the ER and/or the mislocalization of Kar2p, observed in early secretory mutants, is responsible for the reduction in CPY* degradation. Further, we observed that mutations in proteins directly involved in degradation of malfolded proteins (Der1p, Der3/Hrd1p, and Hrd3p) lead to morphological changes of the endoplasmic reticulum and the Golgi, escape of CPY* into the secretory pathway and a slower maturation rate of wild-type CPY.
Taxis
Christof
C
Institut für Biochemie, Universität Stuttgart, 70569 Stuttgart, Germany.
Vogel
Frank
F
Wolf
Dieter H
DH
D003513:Cycloheximide / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000502:physiology*; D005838:Genotype; D006056:Golgi Apparatus / Q000502:physiology*; D007700:Kinetics; D010957:Plasmids; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D015290:Second Messenger Systems / Q000502:physiology
D029701:Saccharomyces cerevisiae Proteins; D003513:Cycloheximide