Isolation of a novel mouse variant of the drs tumor suppressor gene.
The drs gene was isolated as a transformation suppressor against the v-src oncogene. Drs protein has a transmembrane domain and three consensus repeats (CRs) called Sushi motifs in the extracellular domain. The drs gene also has the ability to suppress anchorage-independent growth of human cancer cell lines. In this paper, we report the isolation of a novel variant cDNA of mouse drs (mDRS-2) containing two CRs, in addition to a mouse homolog of drs (mDRS-1) containing three CRs. We investigated the suppressor function of these mDRS cDNAs in human cancer cells and found that the lack of one CR is critical for suppression of anchorage-independent growth by drs.
Kawai
Takao
T
Division of Food Microbiology, Osaka Prefectural Institute of Public Health, Nakamichi, Higashinari-ku, 537-0025, Osaka, Japan.
Suzuki
Yasuhiko
Y
Yamashita
Atsuko
A
Inoue
Hirokazu
H
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D005260:Female; D015723:Gene Library; D016147:Genes, Tumor Suppressor; D016391:Genes, src; D014644:Genetic Variation; D005822:Genetic Vectors; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D010053:Ovary; D012190:Retroviridae; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured
D008565:Membrane Proteins; C486297:SRPX protein, human; C486298:Srpx protein, mouse