Detection of porcine circovirus 2 (PCV-2) DNA by nested PCR from formalin-fixed tissues of post-weaning multisystemic wasting syndrome (PMWS) pigs in Thailand.
A retrospective study for the detection of porcine circovirus 2 (PCV-2) DNA was conducted by nested PCR method in 16 cases of swine post-weaning multisystemic wasting syndrome (PMWS) in Thailand. Histopathology showed characteristic lesions of PMWS and intracytoplasmic viral inclusion bodies in macrophages infiltrating in lymphoid tissues. PCV-2 DNA was detected from formalin-fixed and/or formalin-fixed paraffin-embedded tissues from all pigs with PMWS. The amplified products were digested with Hae III.
Kiatipattanasakul-Banlunara
Wijit
W
Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Tantilertcharoen
Rachod
R
Suzuki
Kazuhiko
K
Albarenque
Stella Maris
SM
Thanawongnuwech
Roongroje
R
Nakayama
Hiroyuki
H
Doi
Kunio
K
D000818:Animals; D018173:Circoviridae Infections / Q000662:veterinary / Q000821:virology*; D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis*; D016133:Polymerase Chain Reaction; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000821:virology*; D019282:Wasting Syndrome / Q000662:veterinary / Q000821:virology*
D004279:DNA, Viral