title: [Preclinical studies for the use of the platelet function analyser PFA-100 with the collagen/ADP cartridge in dogs].
abstract: In this study, the following three aspects of platelet function analyser were investigated in dogs, using a collagen/ADP cartridge: precision, influence of the cartridge batch and of the sample storage time. Closure time and total volume of blood flow until closure of the capillary were measured. Based on several series of 5 repeated measurements mean coefficients of variation were 5% (3-6%; closure time) or 3% (1-5%; total volume). Neither closure time, nor total volume showed significant differences (p > 0.05) when comparing the results of 6 different batches of the collagen/ADP cartridge. Closure time (p = 0.0211, analysis of variance) and total volume (p = 0.0310) were significantly influenced by storage time, based on the sample material of 6 healthy dogs which was stored for 24 hours. Shortening of the closure time and decrease of the total volume observed in the time interval 1-2 hours after blood collection was followed by a significant prolongation of closure time and increase of the total volume (p < 0.05) starting 8 hours after blood collection. This study shows sufficient reproducibility which is not affected by reagent batch number. The results of the studies on storage indicated nearly identical recommendations for storage time before measurement of canine (0.5-2 hours) and human (0.5-3 hours) sample material.
authors.affiliation: Klinik für kleine Haustiere, Tierärztlichen Hochschule Hannover.
authors.forename: R
authors.initials: R
authors.lastname: Mischke
authors.forename: A
authors.initials: A
authors.lastname: Keidel
mesh_terms: D000244:Adenosine Diphosphate; D000818:Animals; D001760:Bleeding Time / Q000662:veterinary; D001792:Blood Platelets / Q000502:physiology*; D001810:Blood Volume; D002196:Capillaries; D003094:Collagen; D004285:Dogs / Q000097:blood*; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D000244:Adenosine Diphosphate; D003094:Collagen
vernacular_title: Präklinische Untersuchungen zur Anwendung des Plättchenfunktionsanalysengerätes PFA-100 mit der Kollagen/ADP-Messzelle beim Hund.