Experimental study on apoptosis of HL-60 cell induced by arsenic trioxide.
OBJECTIVE
To explore the mechanism of arsenic (arsenic trioxide, AS2O3) in treating leukemia.


METHODS
The in vitro effect of arsenic trioxide on HL-60 model double-labelled with PI/AnnexinV-FITC was studied ith flow cytometry. Function on telomerase activity was also observed.


RESULTS
After the effect of arsenic on HL-60 cell line, PI negative/AnnexinV-FITC positive cells increased, it mainly acts at the G2-M phase that suggests the apoptosis takes place, mild inhibition on telomerase was also found 48 hours later.


CONCLUSIONS
Apoptosis induced by arsenic trioxide was the main mechanism in killing or inhibiting leukemic cells.
Wang
Z
Z
Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Bejing (10091).
Xu
Y
Y
Liao
J
J
D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D018922:HL-60 Cells / Q000473:pathology; D006801:Humans; D010087:Oxides / Q000494:pharmacology*; D019098:Telomerase / Q000378:metabolism
D001152:Arsenicals; D010087:Oxides; D019098:Telomerase; D000077237:Arsenic Trioxide