title: Diesel particles are taken up by alveolar type II tumor cells and alter cytokines secretion.
abstract: Diesel exhaust particles can reach the alveolar space and interact with alveolar type II cells. The authors investigated whether diesel exhaust particles lead to an internalization process and alter the production of proinflammatory cytokines, such as interleukin-8 and granulocyte macrophage-colony-stimulating factor by human alveolar type II cells. Cells from the human lung epithelial cell line A-549 were incubated with diesel exhaust particles or with inert particles for different periods of time. Phagocytosis was studied with electron microscopic analysis and flow cytometry. Cytokines were quantified in supernatants with enzyme-linked immunosorbent assay. Both diesel exhaust particles and inert particles were similarly engulfed by alveolar type II cells. Diesel exhaust particles induced a dose- and a time-dependent increase in granulocyte macrophage-colony-stimulating factor release and a transient inhibition of interleukin-8 release, but inert particles did not. Diesel exhaust particles were taken up by alveolar type II cells, and they altered cytokine production. Alveolar type II cells, therefore, may represent a target site for the deleterious effects of diesel exhaust particles.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Paris, France. pjuvinAfree.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Juvin
authors.forename: Thierry
authors.initials: T
authors.lastname: Fournier
authors.forename: Sonja
authors.initials: S
authors.lastname: Boland
authors.forename: Paul
authors.initials: P
authors.lastname: Soler
authors.forename: Francelyne
authors.initials: F
authors.lastname: Marano
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Desmonts
authors.forename: Michel
authors.initials: M
authors.lastname: Aubier
mesh_terms: D002244:Carbon / Q000633:toxicity; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D008854:Microscopy, Electron; D010587:Phagocytosis; D011650:Pulmonary Alveoli / Q000166:cytology*; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D016207:Cytokines; D016209:Interleukin-8; D001335:Vehicle Emissions; D002244:Carbon; D016178:Granulocyte-Macrophage Colony-Stimulating Factor