title: Simultaneous staining of cytoplasmic iron and collagen matrix in human liver biopsy specimens.
abstract: One of the major liver fibrogenic activators is the cellular iron overload that can severely damage parenchymal and non-parenchymal cells. The aim of this study was to investigate a histochemical staining technique that allows the simultaneous detection of the irregular deposition of matrix collagen and both the amount and distribution of iron in liver cells on the same histological section. The method was evaluated using 3-microm histological sections obtained from ten standard liver biopsy specimens taken from patients with hepatitis C virus-related diseases and simultaneous liver cell iron overload. The results indicate that the double-staining technique is simple, sensitive and rapid, and can be routinely applied to liver biopsy specimens for diagnostic purposes. Furthermore, it may also facilitate the study of the complex relationship between hepatic fibrosis and iron overload during common genetic or secondary liver metabolic disorders.
authors.affiliation: Scientific Direction Unit, Istituto Clinico Humanitas, Rozzano, Milan, Italy. fabio.grizzi@humanitatis.it
authors.forename: F
authors.initials: F
authors.lastname: Grizzi
authors.forename: G
authors.initials: G
authors.lastname: Ceva-Grimaldi
authors.forename: B
authors.initials: B
authors.lastname: Franceschini
authors.forename: M
authors.initials: M
authors.lastname: Roncalli
authors.forename: M
authors.initials: M
authors.lastname: Chiriva-Internati
authors.forename: N
authors.initials: N
authors.lastname: Dioguardi
mesh_terms: D001706:Biopsy; D003593:Cytoplasm / Q000378:metabolism* / Q000473:pathology; D006432:Hemochromatosis / Q000378:metabolism / Q000473:pathology; D006486:Hemosiderosis / Q000378:metabolism / Q000473:pathology; D019698:Hepatitis C, Chronic / Q000378:metabolism / Q000473:pathology; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007501:Iron / Q000378:metabolism*; D007728:Kupffer Cells / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D007501:Iron