title: Renal excretion of 8-oxo-7,8-dihydro-2(')-deoxyguanosine: degradation rates of RNA and metabolic rate in humans.
abstract: Renally excreted 8-oxo-7,8-dihydro-2(')-deoxyguanosine (oxo(8)dG) is a potential marker of oxidative DNA damage by reactive oxygen species. Whole-body degradation rates of t- and rRNA are potential indicators of the resting metabolic rate (RMR). Excretion rates of oxo(8)dG and degradation rates of t- and rRNA were determined in healthy non-smoking adults and children. RMR (indirect calorimetry; 14 children, 16 adults), total energy expenditure (TEE; doubly labelled water technique; 4 children, 6 adults), and lean body mass (LBM; dual-energy X-ray absorptiometry; 14 children, 16 adults) were also measured. Degradation of t- and rRNA (micromol/d/kg LBM; 4 children, 6 adults) was highly correlated with RMR (kJ/d/kg LBM), r=0.867 (p<0.005) and 0.959 (p<0.001), respectively. Excretion of oxo(8)dG (pmol/d/kg LBM; 14 children, 16 adults) was not significantly correlated with RMR (p>0.05). Neither excretion of oxo(8)dG nor degradation of RNA was significantly correlated with TEE (kJ/d/ kg LBM) (p>0.05). In healthy subjects further factors, other than the metabolic rate, seem to influence the excretion rate of oxo(8)dG. The degradation rates of t- and rRNA seem to be appropriate indicators of the RMR.
authors.affiliation: Neonatology, University Children's Hospital, Soldtmannstr. 15, D-17487 Greifswald, Germany. topp@uni-greifswald.de
authors.forename: Heinrich
authors.initials: H
authors.lastname: Topp
authors.forename: Sven
authors.initials: S
authors.lastname: Armbrust
authors.forename: Christoph
authors.initials: C
authors.lastname: Lengger
authors.forename: Gerhard
authors.initials: G
authors.lastname: Schöch
authors.forename: Joanne
authors.initials: J
authors.lastname: Davies
authors.forename: Willibald
authors.initials: W
authors.lastname: Stichler
authors.forename: Friedrich
authors.initials: F
authors.lastname: Manz
authors.forename: Christoph
authors.initials: C
authors.lastname: Fusch
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015502:Absorptiometry, Photon; D000328:Adult; D001481:Basal Metabolism / Q000502:physiology*; D002153:Calorimetry, Indirect; D002648:Child; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress; D012335:RNA, Ribosomal / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D012335:RNA, Ribosomal; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D012343:RNA, Transfer; D003849:Deoxyguanosine