Expression of fibronectin-binding protein FbpA modulates adhesion in Streptococcus gordonii.
Fibronectin binding is considered to be an important virulence factor in streptococcal infections. Adhesion of the oral bacterium Streptococcus gordonii to immobilized forms of fibronectin is mediated, in part, by a high molecular mass wall-anchored protein designated CshA. In this study, a second fibronectin-binding protein of S. gordonii is described that has been designated as FbpA (62.7 kDa). This protein, which is encoded by a gene located immediately downstream of the cshA gene, shows 85 and 81% identity to the fibronectin-binding proteins PavA, of Streptococcus pneumoniae, and FBP54, of Streptococcus pyogenes, respectively. Purified recombinant FbpA bound to immobilized human fibronectin in a dose-dependant manner, and isogenic mutants in which the fbpA gene was inactivated were impaired in their binding to fibronectin. This effect was apparent only for cells in the exponential phase of growth, and was associated with reduced surface hydrophobicity and the surface expression of CshA. Cells in the stationary phase of growth were unaffected in their ability to bind to fibronectin. By utilizing gene promoter fusions with cat (encoding chloramphenicol O-acetyltransferase), it was demonstrated that cshA expression was down-regulated during the exponential phase of growth in the fbpA mutant. Expression of fbpA, but not cshA, was sensitive to atmospheric O2 levels, and was found to be up-regulated in the presence of elevated O2 levels. The results suggest that FbpA plays a regulatory role in the modulation of CshA expression and, thus, affects the adhesion of S. gordonii to fibronectin.
Christie
Julie
J
Department of Oral and Dental Science, University of Bristol, Lower Maudlin St, Bristol BS1 2LY, UK1.
McNab
Roderick
R
Department of Microbiology, Eastman Dental Institute, University College London, 256 Gray's Inn Rd, London WC1X 8LD, UK2.
Jenkinson
Howard F
HF
Department of Oral and Dental Science, University of Bristol, Lower Maudlin St, Bristol BS1 2LY, UK1.
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D004777:Environment; D005353:Fibronectins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010100:Oxygen / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D013291:Streptococcus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; C079041:CshA protein, Streptococcus gordonii; D005353:Fibronectins; C119770:FlpA protein, bacteria; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C093280:fibrinogen-binding protein A, Staphylococcus aureus; D010100:Oxygen