title: Isolation and characterization of Leishmania infantum cDNA encoding a protein homologous to eukaryotic elongation factor 1 gamma.
abstract: This paper reports the isolation and characterization of a complementary deoxyribonucleic acid clone showing sequence homology with genes coding for the eukaryotic elongation factor 1 gamma (EF-1 gamma). The clone encodes an open reading frame of 404 amino acids corresponding to a deduced molecular mass of 46.2 kDa. Database searches revealed 30-64% sequence identity between the Leishmania infantum EF-1 gamma and several eukaryotic homologues. Southern blot analysis indicated that 2 genes tandemly organized were present in the L. infantum genome. The 3' untranslated regions of these 2 genes differed in size. Southern hybridization and pulsed field gel electrophoresis showed that EF-1 gamma genes are highly conserved among members of the Leishmania genus and must be clustered in a single chromosomal locus.
authors.affiliation: WHO Collaborating Centre for Leishmaniasis, Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo km. 2, 28220 Majadahonda, Madrid, Spain. ccanave@isciii.es
authors.forename: Carmen
authors.initials: C
authors.lastname: Cañavate
authors.forename: Mohammed
authors.initials: M
authors.lastname: el Bali
authors.forename: Ysabel
authors.initials: Y
authors.lastname: Montoya
authors.forename: Douglas C
authors.initials: DC
authors.lastname: Barker
authors.forename: Jorge
authors.initials: J
authors.lastname: Alvar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field; D018503:Genome, Protozoan; D006801:Humans; D018314:Leishmania infantum / Q000235:genetics*; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D016054:DNA, Protozoan; D020648:Peptide Elongation Factor 1; D015800:Protozoan Proteins