Nerve growth factor activates mast cells through the collaborative interaction with lysophosphatidylserine expressed on the membrane surface of activated platelets.
Effect of nerve growth factor (NGF) on platelet-associated mast cell activation was investigated. Although neither NGF alone nor platelets alone induced significant 5-hydroxytriptamine (5-HT) release from rat peritoneal mast cells, marked 5-HT release was detected when costimulated with NGF and calcium ionophore-activated platelets. This response reached maximal levels as early as 5 min after the initiation of the coincubation and was completely blocked by anti-NGF Ab or by an inhibitor for a tyrosine kinase of the trkA NGF receptor. Paraformaldehyde-fixed platelets activated with either calcium ionophore or thrombin exhibited the collaborative ability, suggesting the possible involvement of some membrane molecules expressed on activated platelets in mast cell activation. Because activation of platelets induced expression of phosphatidylserine (PS) and/or lysoPS on membrane surface, and since lysoPS, unlike PS, initiated the NGF-induced 5-HT release, lysoPS expressed on activated platelets may be involved in the mast cell activation. Moreover, intradermal injection of NGF and activated platelets into the rat skin increased local vascular permeability. These findings suggested that NGF collaboratively worked with membrane lysoPS of activated platelets to induce mast cell activation. Thus, NGF released in response to inflammatory stimuli may contribute to mast cell activation in collaboration with locally activated platelets in the process of inflammations and tissue repair.
Kawamoto
Keiko
K
Faculty of Agriculture, Department of Veterinary Clinic, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Aoki
Junken
J
Tanaka
Akane
A
Itakura
Atsuko
A
Hosono
Hiroyuki
H
Arai
Hiroyuki
H
Kiso
Yasuo
Y
Matsuda
Hiroshi
H
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002199:Capillary Permeability / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D007271:Injections, Intradermal; D008246:Lysophospholipids / Q000096:biosynthesis / Q000502:physiology*; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D008563:Membrane Lipids / Q000096:biosynthesis / Q000502:physiology*; D051379:Mice; D020932:Nerve Growth Factor / Q000008:administration & dosage / Q000502:physiology*; D010537:Peritoneum / Q000166:cytology; D010718:Phosphatidylserines / Q000494:pharmacology; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology*; D017713:Platelet Transfusion; D011108:Polymers; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D014407:Tumor Cells, Cultured
D016207:Cytokines; D005404:Fixatives; D008246:Lysophospholipids; D008563:Membrane Lipids; D010718:Phosphatidylserines; D011108:Polymers; D012333:RNA, Messenger; C025059:lysophosphatidylserine; D005557:Formaldehyde; D012701:Serotonin; D020932:Nerve Growth Factor; D002118:Calcium; C003043:paraform