Prenatal LHRH neurons in nasal explant cultures express estrogen receptor beta transcript.
Sex steroids influence LHRH neuronal activity, exerting a negative or positive feedback action, depending on the reproductive state of the animal. Recent evidence indicates that LHRH neurons possess the estrogen receptor beta (ERbeta) subtype postnatally, suggesting that estrogen may act, in part, directly on LHRH neurons. In this study, we identified ERbeta transcript in prenatal LHRH neurons as a function of age. Single-cell cDNA pools were made from LHRH neurons maintained for 7, 14, and 28 d in vitro (div). Screening of the cDNA pools by PCR with ERbeta-specific primers revealed ERbeta-positive LHRH neurons at all three ages. However, the number of LHRH cells coexpressing ERbeta transcript decreased dramatically between 14 (6/10) and 28 div (1/10). None of the LHRH cells were positive for ERalpha transcript. These results indicate that developing LHRH neurons express the transcript for ERbeta and suggest that continued expression of ERbeta is either a characteristic of LHRH neurons that may require cues from the central nervous system and/or periphery or predetermined to be maintained in a subpopulation of LHRH neurons.
Sharifi
Neda
N
Cellular and Developmental Neurobiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.
Reuss
Andree E
AE
Wray
Susan
S
D000818:Animals; D002469:Cell Separation; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D005314:Embryonic and Fetal Development / Q000502:physiology; D047629:Estrogen Receptor beta; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000502:physiology*; D007150:Immunohistochemistry; D051379:Mice; D009297:Nasal Mucosa / Q000196:embryology / Q000294:innervation* / Q000378:metabolism*; D009412:Nerve Fibers / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009924:Organ Culture Techniques; D011247:Pregnancy; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D014158:Transcription, Genetic
D018076:DNA, Complementary; D047629:Estrogen Receptor beta; D011960:Receptors, Estrogen; D007987:Gonadotropin-Releasing Hormone