title: Polyamine uptake is necessary for a normal biochemical maturation of astrocytes in culture.
abstract: In this study we have explored the importance of polyamine uptake in the proliferation and biochemical maturation of cerebellar astroyctes in culture. The uptake of polyamines paralleled astrocyte proliferation measured as [3H]thymidine incorporation into the DNA. Inhibition of polyamine uptake did not alter the developmental profile of thymidine incorporation, perhaps due to a compensatory increase in ornithine decarboxylase activity but was able to reduce glutamine synthetase (GS) activity, an enzymatic marker for astrocyte biochemical maturation, from 9 days in vitro. The present results suggest that polyamine uptake plays an important role in the biochemical maturation of astrocytes in culture.
authors.affiliation: Instituto de Neurociencias and Departamento de Bioquímica y Biología Molecular, Edificio M, Universidad Autónoma de Barcelona,08193 Cerdanyola del Vallés, Barcelona, Spain.
authors.forename: Josep
authors.initials: J
authors.lastname: Dot
authors.forename: Nikolai
authors.initials: N
authors.lastname: Danchev
authors.forename: Isaac
authors.initials: I
authors.lastname: Blanco
authors.forename: José
authors.initials: J
authors.lastname: Rodríguez-Alvarez
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000201:enzymology*; D015317:Biogenic Polyamines / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D004247:DNA / Q000096:biosynthesis; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D000518:Eflornithine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D051379:Mice; D008935:Mitoguazone / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology; D013095:Spermidine / Q000378:metabolism / Q000494:pharmacology; D013096:Spermine / Q000378:metabolism / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
chemical_list: D015317:Biogenic Polyamines; D004791:Enzyme Inhibitors; D013096:Spermine; D004247:DNA; D009955:Ornithine Decarboxylase; D005974:Glutamate-Ammonia Ligase; D008935:Mitoguazone; D013095:Spermidine; D011700:Putrescine; D013936:Thymidine; D000518:Eflornithine