title: Nigella sativa oil, nigellone and derived thymoquinone inhibit synthesis of 5-lipoxygenase products in polymorphonuclear leukocytes from rats.
abstract: In the present study, Nigella sativa oil (NSO), nigellone (polythymoquinone) and derived thymoquinone were studied to evaluate their effect on the formation of 5-lipoxygenase (5-LO) products from polymorphonuclear leukocytes (PMNL).NSO produced a concentration dependent inhibition of 5-LO products and 5-hydroxy-eicosa-tetra-enoic acid (5-HETE) production with half maximal effects (IC(50)) at 25+/-1 micro g/ml, respectively 24+/-1 micro g/ml. Nigellone caused a concentration-related inhibition of 5-HETE production (IC(50): 11.9+/-0.3 micro g/ml). Moreover thymoquinone, the active principle of NSO inhibited the production of 5-LO products (IC(50): 0.26+/-0.02 micro g/ml) and 5-HETE production (IC(50): 0.36+/-0.02 micro g/ml) in a similar way. The effects are probably due to an antioxidative action. The data may in part explain the effect of the oil, its derived thymoquinone and nigellone in ameliorating inflammatory diseases.
authors.affiliation: Department of Pharmacology, Alexandria Medical Faculty, Alexandria, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: El-Dakhakhny
authors.forename: N J
authors.initials: NJ
authors.lastname: Madi
authors.forename: N
authors.initials: N
authors.lastname: Lembert
authors.forename: H P T
authors.initials: HP
authors.lastname: Ammon
mesh_terms: D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000096:biosynthesis; D016227:Benzoquinones / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016859:Lipoxygenase Inhibitors; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds; D029974:Carum
chemical_list: D016227:Benzoquinones; D016859:Lipoxygenase Inhibitors; D010938:Plant Oils; C080157:nigellone; C104525:caraway oil; D001094:Arachidonate 5-Lipoxygenase; C003466:thymoquinone