Morphological study of organotypic cerebellar cultures.
Organotypic cerebellar cultures from 8-days-old (P8) mouse pups were studied following 11 days of in vitro (I IDIV) culturing. The cerebellar cytoarchitectonic structure was maintained in most parasagittal cerebellar cortical slice cultures (also containing the deep cerebellar nuclei). The two main extrinsic excitatory inputs (the climbing and the mossy fibers) seem to be replaced by other axonal types: in the molecular layer mostly by parallel fibers (for climbing fibers) and in the granular layer by intrinsic mossy fiber collaterals of local excitatory interneurons, the unipolar brush cells. However, in a few organotypic cultures, which (although preserving the trilaminar cerebellar cortical structure) were "granuloprival" but also contained some of the deep cerebellar nuclei, the participation of extracortical axons from the deep cerebellar nuclei in the replacement of the missing afferents is suggested.
Takács
J
J
Neurobiology Research Group, United Research Organization of the Hungarian Academy of Sciences and Semmelweis University, Budapest. takacs@ana.sote.hu
Metzger
F
F
D000344:Afferent Pathways / Q000033:anatomy & histology; D000818:Animals; D002525:Cerebellar Cortex / Q000033:anatomy & histology / Q000502:physiology / Q000648:ultrastructure; D002531:Cerebellum / Q000033:anatomy & histology* / Q000502:physiology / Q000648:ultrastructure; D007150:Immunohistochemistry; D051379:Mice; D008854:Microscopy, Electron; D009473:Neuronal Plasticity; D009924:Organ Culture Techniques; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism; D013569:Synapses / Q000648:ultrastructure
D018094:Receptors, Metabotropic Glutamate; C104077:metabotropic glutamate receptor type 1