A method for measuring oxygen distributions in tissue using frequency domain phosphorometry.
Phosphorescence lifetime of phosphors dissolved in biological fluids is dependent on the local oxygen concentration. When phosphor is added to the blood in vivo there is a distribution of lifetimes due to the distribution of oxygen concentrations in the blood of the sampled tissue volume. The distribution of oxygen can be assessed non-invasively and in practically real-time using frequency domain time-resolved phosphorometry. Here we describe a multi-frequency system for measurements of oxygen distributions in tissue in vivo.
Vinogradov
Sergei A
SA
Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. vinograd@mail.med.upenn.edu
Fernandez-Seara
Maria A
MA
Dupan
Benjamin W
BW
Wilson
David F
DF
D019277:Entropy; D008163:Luminescent Measurements; D008433:Mathematics; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism*; D012984:Software; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation / Q000379:methods*
D010100:Oxygen