Liposome clearance in mice: the effect of a separate and combined presence of surface charge and polymer coating.
The purpose of our work was to compare the biodistribution of liposomes with different surface properties. Phosphatidylcholine (PC)/cholesterol (Chol) liposomes were prepared containing 6% mol of a charged lipid (stearylamine, SA; phosphatidic acid, PA; or phosphatidyl serine, PS) and/or polyethylene glycol (PEG)-PE of different MW (750 and 5000). zeta-Potentials and liposome clearance in mice were investigated. In vitro, the attachment of PEG in a similar fashion neutralizes the effect of any charged component. In vivo, the chemical nature of a charged lipid becomes important. Both short PEG750 and longer PEG5000 inhibit the clearance of positively charged SA-liposomes, while only longer PEG5000 inhibits the clearance of negatively charged PA-liposomes and none of the PEGs inhibit the clearance of negatively charged PS-liposomes. The opsonins with different molecular size may be involved in the clearance of liposomes containing different charged lipids.
Levchenko
Tatiana S
TS
Department of Pharmaceutical Sciences, Bouve College of Health Sciences, Northeastern University, Mugar Building 312, 360 Huntington Avenue, Boston, MA 02115, USA.
Rammohan
Ram
R
Lukyanov
Anatoly N
AN
Whiteman
Kathleen R
KR
Torchilin
Vladimir P
VP
D000818:Animals; D004563:Electrochemistry; D008081:Liposomes / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism; D008657:Metabolic Clearance Rate; D051379:Mice; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties; D014018:Tissue Distribution
D008081:Liposomes; D011092:Polyethylene Glycols