title: Improved blunt-end cloning by replacing EcoRV with Eco32I.
authors.affiliation: Department of Pathology, University Hospital of Heidelberg, Germany. r.koesters@dkfz.de
authors.forename: Robert
authors.initials: R
authors.lastname: Koesters
authors.forename: Magnus
authors.initials: M
authors.lastname: von Knebel Doeberitz
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D003598:Cytoskeletal Proteins / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D006801:Humans; D007612:Kanamycin / Q000378:metabolism; D016133:Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics; D051176:beta Catenin
chemical_list: C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D051176:beta Catenin; D007612:Kanamycin; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases