The effect of prolonged ischaemia and cryopreservation on the cell viability of human aortic and femoral artery allografts.
The viability of the human arterial allograft cells depends on the time and method of vessel procurement and storage. In this study, an evaluation of the effect of the duration of 4 degrees C ischaemia and cryopreservation on human aortic and femoral artery allograft viability was performed. After the isolation of arterial wall cells, the identification of cultured cells was performed using mRNA analysis for estimation of smooth-muscle markers of differentiation: desmin and heavy-caldesmon. The viability of cells from the medial layer of the aortic wall ranged from 74 to 90% (61-79% for femoral arteries). Cold ischaemia time (from harvesting until the beginning of the preparation) is a statistically significant factor influencing smooth muscle cell viability. Smooth muscle cells represented the majority of live cell population.
Skop
B
B
Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, Sosnowiec, Poland. bskop@slam.katowice.pl
Urbanek
T
T
Bursig
H
H
Ziaja
K
K
Wilczok
T
T
D000293:Adolescent; D000328:Adult; D001011:Aorta / Q000033:anatomy & histology / Q000502:physiology* / Q000637:transplantation; D002455:Cell Division / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation; D005260:Female; D005263:Femoral Artery / Q000033:anatomy & histology / Q000502:physiology* / Q000637:transplantation; D006801:Humans; D007511:Ischemia / Q000473:pathology; D008297:Male; D009130:Muscle, Smooth / Q000166:cytology / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014182:Transplantation, Autologous