The restoring effect of trifluralin and benznidazole on the abnormal fatty-acid pattern induced by Trypanosoma cruzi in the liver microsomes of infected mice.
The fatty-acid composition of liver lipids from mice infected with Trypanosoma cruzi (clone H510C8C3) or uninfected mice was investigated. The infected animals were treated orally for 30 days, with trifluralin (TFL) or benznidazole (BNZ), each at 100mg/kg.day, or only with the peanut oil used as the drug vehicle. The uninfected mice were also given the peanut oil. The treatments were stopped 10 days before the animals were killed. The liver microsomal lipids of each mouse were isolated and then analysed by gas-liquid chromatography. In terms of the total lipids, untreated infection evoked a significant increase in saturated fatty acids and the members of the n-9 fatty-acid family, with a concomitant decrease in the polyenoates of the n-3 and n-6 fatty-acid series. Each lipid subclass was affected to a different extent, the phospholipids being affected most. All lipid fractions, apart from the cholesterol esters, showed a significant increase in the proportion of n-9 isomers. Infection also produced a marked increase in the absolute amounts of triacylglycerides, cholesterol and cholesterol esters in liver microsomal membranes. After BNZ or TFL treatment, the fatty-acid pattern of mice that had been infected was indistinguishable from that of the control mice. The possible role of desaturase activity in the alterations observed is discussed.
Marra
C A
CA
INIBIOLP (Instituto de Investigaciones Bioquímicas de La Plata), Cátedra de Bioquímica, Universidad Nacional de La Plata, Calle 60 y 120, 1900, Argentina. camarra@atlas.med.unlp.edu.ar
Zaidenberg
A
A
de Alaniz
M J T
MJ
Buschiazzo
H
H
D000818:Animals; D014355:Chagas Disease / Q000188:drug therapy / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D009593:Nitroimidazoles / Q000627:therapeutic use*; D014274:Trifluralin / Q000627:therapeutic use*; D014280:Triglycerides / Q000378:metabolism; D014344:Trypanocidal Agents / Q000627:therapeutic use*
D002788:Cholesterol Esters; D005227:Fatty Acids; D009593:Nitroimidazoles; D014280:Triglycerides; D014344:Trypanocidal Agents; D002784:Cholesterol; D014274:Trifluralin; C009999:benzonidazole