title: Determination of benzimidazole anthelmintics in animal-derived biological matrices.
abstract: This paper describes an easy multiresidue procedure for the determination of 8 benzimidazole anthelmintics (Albendazole, Albendazole sulphone, Albendazole sulphoxide, Ox-bendazole, Ossibendazole, p-OH-Fenbendazole, Fenbendazole, Flubendazole) in foodstuffs of animal origin for human consumption. According to the proposed procedure, 10 g of homogenised sample were extracted with acetonitrile in ultrasonic bath, the organic layer was evaporated to dryness and the residue dissolved in HCl 0.005 M. After an initial washing with hexane to remove fats, the aqueous layer was purified on SPE C2 column and the analytes were eluted with methanol, evaporated to dryness and reconstituted with phosphate buffer/acetonitrile (50/50). The solution obtained was analysed by HPLC with diode array detector (DAD); this detector provides useful information about the peaks present in routine samples, because it gains not only retention times of analytes, as well as their UV-spectra. Benzthiazuron was used as internal standard and the method was validated in the range 10-100 ppb, as requested by the imposed limits. The mean recoveries in spiked samples ranged between 70% and 95%, for 10 and 100 ppb respectively. The developed method resulted sensitive, simple and fast. It is particularly suitable for laboratories that execute screening analysis on a great number of samples, observing the EC regulations that establish the benzimidazole Maximum Residue Limits (MRLs) in food.
authors.affiliation: Istituto Zooprofilattico Sperimentale Lazio e Toscana, Via Appia Nuova, 1411, 00178 Roma. bneri@rm.izs.it
authors.forename: Bruno
authors.initials: B
authors.lastname: Neri
authors.forename: Gentilina
authors.initials: G
authors.lastname: Bidolli
authors.forename: Marialinda
authors.initials: M
authors.lastname: Felli
authors.forename: Roberto
authors.initials: R
authors.lastname: Cozzani
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology; D000871:Anthelmintics / Q000032:analysis*; D001562:Benzimidazoles / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination; D008460:Meat / Q000032:analysis*; D012016:Reference Values
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles