An observation of the initial stage towards a symbiotic relationship.
Two well-characterized and phylogeneticaly different species, Escherichia coli and Dictyostelium discoideum, were used as the model organisms. When the two species were mixed and allowed to grow on minimal agar plates at 22 degrees C, remarkably, the two species achieved a state of coexistence at an average of 2-4 weeks. In addition, the emerged colonies housing the coexisting species had a mucoidal nature that was not observed from its origin. Moreover, the state of coexistence was confirmed to be stable, and so was the mucoidal nature of the emerged colonies. Comparing with the pure E. coli origin, the mucoidal colony showed a significant increase in higher molecular weight extracellular components, with polysaccharides as the major constituent. Qualitative analysis of the monosaccharide contents in the extracellular components of the mucoidal colony revealed not only a significant increase in the glucose content, but also significant amount of additional xylose and galactose. The system permits the initial stages of the development of relationship between two species be captured within a short period of time. This feature, together with being simple and reproducible in laboratory conditions, provides a new model system for the study of symbiosis, especially when initial stages are concerned.
Todoriki
Masahiko
M
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita City, Osaka 565-0871, Japan.
Oki
Shinya
S
Matsuyama
Shin-Ichi
S
Ko-Mitamura
Elizabeth P
EP
Urabe
Itaru
I
Yomo
Tetsuya
T
D000818:Animals; D004023:Dictyostelium / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology*; D013559:Symbiosis