Determination of khellin and visnagin in Ammi visnaga fruits by capillary electrophoresis.
A new, simple and rapid capillary electrophoresis method was developed for the identification and quantitative determination of two medically active constituents-khellin and visnagin-in the extracts of Ammi visnaga fruits. Micellar electrochromatographic separation of khellin and visnagin was carried out using 10 mmol/l borate, 50 mmol/l sodium dodecylsulfate, 25% (v/v) acetonitrile at pH 9 as running buffer. Ammi visnaga fruits were extracted with methanol and the extracts were directly injected without any purification and pre-separation processes. Coumarin was used as internal standard for quantitation and the limits of detection for khellin and visnagin were 2.36 and 1.97 microg/ml, respectively using UV detection at 245 nm.
Günaydin
K
K
Department of Chemistry, Mustafa Kemal University, Antakya, Hatay, Turkey.
Erim
F B
FB
D031165:Ammi / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007666:Khellin / Q000031:analogs & derivatives* / Q000032:analysis*; D012680:Sensitivity and Specificity
D007666:Khellin; C044999:visnagin