Human chromosomes 9, 12, and 15 contain the nucleation sites of stress-induced nuclear bodies.
We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Serine-Arginine splicing factors. In this article, we show that stress-induced SNBs are detectable in human but not in hamster cells. By means of hamster>human cell hybrids, we have identified three human chromosomes (9, 12, and 15) that are individually able to direct the formation of stress bodies in hamster cells. Similarly to stress-induced SNB, these bodies are sites of accumulation of hnRNP A1-interacting protein and heat shock factor 1, are usually associated to nucleoli, and consist of clusters of perichromatin granules. We show that the p13-q13 region of human chromosome 9 is sufficient to direct the formation of stress bodies in hamster>human cell hybrids. Fluorescence in situ hybridization experiments demonstrate that the pericentromeric heterochromatic q12 band of chromosome 9 and the centromeric regions of chromosomes 12 and 15 colocalize with stress-induced SNBs in human cells. Our data indicate that human chromosomes 9, 12, and 15 contain the nucleation sites of stress bodies in heat-shocked HeLa cells.
Denegri
Marco
M
Istituto di Genetica Molecolare del Consiglio Nazionale delle Ricerche, 27100 Pavia, Italy. biamonti@gbe.pv.cnr.it
Moralli
Daniela
D
Rocchi
Mariano
M
Biggiogera
Marco
M
Raimondi
Elena
E
Cobianchi
Fabio
F
De Carli
Luigi
L
Riva
Silvano
S
Biamonti
Giuseppe
G
D016475:3T3 Cells; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002522:Chlorocebus aethiops; D002881:Chromosomes, Human, Pair 12 / Q000648:ultrastructure*; D002884:Chromosomes, Human, Pair 15 / Q000648:ultrastructure*; D002899:Chromosomes, Human, Pair 9 / Q000648:ultrastructure*; D006224:Cricetinae; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006570:Heterochromatin / Q000648:ultrastructure*; D006801:Humans; D051379:Mice; D008854:Microscopy, Electron; D008938:Mitosis / Q000502:physiology; D013314:Stress, Mechanical; D014162:Transfection
D006570:Heterochromatin