title: Cloning and Expression of Actin Gene of Schistosoma japonicum Chinese Strain.
abstract: A 1 131 bp cDNA fragment was amplified by RT-PCR from adult Schistosoma japonicum(Chinese strain) mRNA with primers designed according to published SmAct2 encoding Schistosoma mansoni actin. Sequence analysis indicated that this fragment, with 92% homology to SmAct2, was a complete open reading fragment (ORF) of actin gene of Schistosoma japonicum (Chinese strain). This gene was cloned into the expression vector pET28a( ) and subsequently expressed in Escerichia coli. SDS-PAGE revealed that the molecular weight of this expressed product was 47 kD. Western blotting showed that the recombinant protein had good reactivity with the rabbit serum immunized with Sj worm antigen, indicating that this gene encode actin of Schistosoma japonicum(Chinese strain).
authors.affiliation: Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Science, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai 200232, China. zjgzylzh@online.sh.cn
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zhu
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Lin
authors.forename: Xing-Gang
authors.initials: XG
authors.lastname: Feng
authors.forename: Xiang-Fu
authors.initials: XF
authors.lastname: Wu
authors.forename: Yuan-Cong
authors.initials: YC
authors.lastname: Zhou
authors.forename: You-Ming
authors.initials: YM
authors.lastname: Cai