title: Betaig-h3 supports keratinocyte adhesion, migration, and proliferation through alpha3beta1 integrin.
abstract: betaig-h3 is an extracellular matrix protein and its expression is highly induced by TGF-beta and it has also been suggested to play important roles in skin wound healing. In this paper, we demonstrate that betaig-h3 is present in the papillary layer of dermis and synthesized in the basal keratinocytes in vivo and its expression is induced by TGF-beta in normal human keratinocytes (NHEK) and HaCaT cells. betaig-h3 mediates not only adhesion and spreading of keratinocytes but also supports migration and proliferation. These activities are mediated through interacting with alpha3beta1 integrin. Previously identified two alpha3beta1 integrin-interacting motifs of betaig-h3, EPDIM, and NKDIL, are responsible for these activities. The results suggest that betaig-h3 may regulate keratinocyte functions in normal skin and potentially during wound-healing process.
authors.affiliation: Department of Biochemistry, Kyungpook National University School of Medicine, Taegu 700-422, Republic of Korea.
authors.forename: Jong-Sup
authors.initials: JS
authors.lastname: Bae
authors.forename: Suk-Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Je-Yong
authors.initials: JY
authors.lastname: Choi
authors.forename: Rang-Woon
authors.initials: RW
authors.lastname: Park
authors.forename: Jae
authors.initials: J
authors.lastname: Yong Park
authors.forename: Hyun-Sook
authors.initials: HS
authors.lastname: Park
authors.forename: Young-Sook
authors.initials: YS
authors.lastname: Sohn
authors.forename: Dong-Sin
authors.initials: DS
authors.lastname: Lee
authors.forename: Eunhee
authors.initials: E
authors.lastname: Bae Lee
authors.forename: In-San
authors.initials: IS
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002465:Cell Movement; D002478:Cells, Cultured; D017124:Conserved Sequence; D020405:Dermis / Q000033:anatomy & histology / Q000737:chemistry; D016326:Extracellular Matrix Proteins; D006801:Humans; D039201:Integrin alpha3beta1; D016023:Integrins / Q000502:physiology*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D000099222:betaIG-H3 Protein
chemical_list: D016326:Extracellular Matrix Proteins; D039201:Integrin alpha3beta1; D016023:Integrins; D009363:Neoplasm Proteins; D010455:Peptides; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein