title: Crystallization and preliminary crystallographic analysis of manganese superoxide dismutase from Bacillus halodenitrificans.
abstract: Manganese superoxide dismutase (GP-MnSOD), a component of the so-called 'green protein' (green protein complex) from the facultative anaerobic halodenitrifier Bacillus halodenitrificans, has been crystallized using the hanging-drop vapor diffusion method. Crystals have unit-cell parameters a=b=93.4 A, c=65.0 A, and belong to the space group P4(3)2(1)2. Preliminary analysis indicates there is one monomer in each asymmetric unit. The structural information from this enzyme will enrich our knowledge on its high catalytic activity and its possible role in green protein complex.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liao
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Ming-Yih
authors.initials: MY
authors.lastname: Liu
authors.forename: Tschining
authors.initials: T
authors.lastname: Chang
authors.forename: Jean
authors.initials: J
authors.lastname: Le Gall
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Gui
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Zhang
authors.forename: Dong-Cai
authors.initials: DC
authors.lastname: Liang
authors.forename: Wen-Rui
authors.initials: WR
authors.lastname: Chang
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D013482:Superoxide Dismutase / Q000737:chemistry*
chemical_list: D013482:Superoxide Dismutase