The potential use of a viral coat protein gene as a transgene screening marker and multiple virus resistance of pepper plants coexpressing coat proteins of cucumber mosaic virus and tomato mosaic virus.
Transgenic pepper plants coexpressing coat proteins (CPs) of cucumber mosaic virus (CMV-Kor) and tomato mosaic virus (ToMV) were produced by Agrobacterium-mediated transformation. To facilitate selection for positive transformants in transgenic peppers carrying an L gene, we developed a simple and effective screening procedure using hypersensitive response upon ToMV challenge inoculation. In this procedure, positive transformants could be clearly differentiated from the nontransformed plants. Transgenic pepper plants expressing the CP genes of both viruses were tested for resistance against CMV-Kor and pepper mild mottle virus (PMMV). In most transgenic plants, viral propagation was substantially retarded when compared to the nontransgenic plants. These experiments demonstrate that our transgenic pepper plants might be a useful marker system for the transgene screening and useful for classical breeding programs of developing virus resistant hot pepper plants.
Shin
Ryoung
R
Graduate School of Biotechnology, Korea University, Seoul.
Han
Jung-Heon
JH
Lee
Gil-Je
GJ
Peak
Kyung-Hee
KH
D016960:Agrobacterium tumefaciens / Q000235:genetics; D002212:Capsicum / Q000235:genetics* / Q000821:virology; D036022:Capsid Proteins / Q000235:genetics*; D017799:Cucumovirus / Q000235:genetics*; D005819:Genetic Markers; D005822:Genetic Vectors; D030821:Plants, Genetically Modified; D017898:Tobamovirus / Q000235:genetics*
D036022:Capsid Proteins; D005819:Genetic Markers