Derris (Lonchocarpus) urucu (Leguminosae) extract modifies the peritrophic matrix structure of Aedes aegypti (Diptera:Culicidae).
Aqueous suspension of ethanol extracts of Derris (Lonchocarpus) urucu (Leguminosae), collected in the state of Amazonas, Brazil, were tested for larvicidal activity against the mosquito Aedes aegypti (Diptera:Culicidae). The aim of this study was to observe the alterations of peritrophic matrix in Ae. aegypti larvae treated with an aqueous suspension of D. urucu extract. Different concentrations of D. urucu root extract were tested against fourth instar larvae. One hundred percent mortality was observed at 150 microg/ml (LC(50) 17.6 microg/ml) 24 h following treatment. In response to D. urucu feeding, larvae excreted a large amount of amorphous feces, while control larvae did not produce feces during the assay period. Ultrastructural studies showed tha larvae fed with 150 microg/ml of D. urucu extract for 4 h have an imperfect peritrophic matrix and extensive damage of the midgut epithelium. Data indicate a protective role for the peritrophic matrix. The structural modification of the peritrophic matrix is intrinsically associated with larval mortality.
Gusmão
Desiely Silva
DS
Laboratorio de Biotecnologia, Centro de Biociências e Biotecnologia, Campos dos Goytacazes, RJ, Brazil.
Páscoa
Valéria
V
Mathias
Leda
L
Curcino Vieira
Ivo José
IJ
Braz-Filho
Raimundo
R
Alves Lemos
Francisco José
FJ
D000330:Aedes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D007887:Fabaceae / Q000737:chemistry*; D007814:Larva / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
D010936:Plant Extracts