title: Cytotoxic and cytostatic effects induced by 4-hydroxynonenal in human osteosarcoma cells.
abstract: Several studies point to the existence of an inverse correlation between cellular lipid peroxidation and both cell proliferation and neoplastic transformation. Furthermore, numerous results demonstrate that lipid peroxidation products affect central biochemical pathways and intracellular signalling at physiological concentrations. 4-Hydroxynonenal (HNE) is one of the most active products of lipid peroxidation. This work has focused on the evaluation of HNE nuclear content, so far never directly measured, by electrospray-ionization-mass-spectrometry (ESI/MS) and on the correlation between its concentration and the induced effects after exogenous administration. In a human osteosarcoma cell line (SaOS2), HNE exhibited an early cytotoxic effect characterized by apoptosis, cytostatic and differentiating effects characterized by slow growth, increase in alkaline phosphatase (ALP), and alpha5 integrin subunit content with decrease in tumorigenicity.
authors.affiliation: Department of Biochemistry "G. Moruzzi," University of Bologna, Bologna, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Calonghi
authors.forename: C
authors.initials: C
authors.lastname: Boga
authors.forename: C
authors.initials: C
authors.lastname: Cappadone
authors.forename: E
authors.initials: E
authors.lastname: Pagnotta
authors.forename: C
authors.initials: C
authors.lastname: Bertucci
authors.forename: J
authors.initials: J
authors.lastname: Fiori
authors.forename: L
authors.initials: L
authors.lastname: Masotti
mesh_terms: D000447:Aldehydes / Q000494:pharmacology* / Q000633:toxicity; D015703:Antigens, CD / Q000378:metabolism; D017209:Apoptosis; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology* / Q000633:toxicity; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D039482:Integrin alpha5; D007700:Kinetics; D015227:Lipid Peroxidation; D018613:Microscopy, Confocal; D012516:Osteosarcoma / Q000188:drug therapy* / Q000378:metabolism; D018384:Oxidative Stress; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D000447:Aldehydes; D015703:Antigens, CD; D002843:Chromatin; D015853:Cysteine Proteinase Inhibitors; D039482:Integrin alpha5; C027576:4-hydroxy-2-nonenal