title: Establishment and characterization of human metastatic hepatocellular carcinoma cell line.
abstract: Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide with a poor prognosis. Recently, we established a HCC cell line from a metastatic HCC tumor. GTG banding analysis was performed and the karyotype showed that this metastatic HCC cell line is a hypertriploid (71-78 chromosomes) with a large marker chromosome containing a long homogeneously staining region (hsr). Comparative genomic hybridization was applied to characterize the chromosomal alterations in this metastatic HCC cell line. The results showed that the hsr was composed of amplified DNA sequences from 11q13. Further characterization of the hsr may lead to the isolation of the putative amplified oncogene at 11q13.
authors.affiliation: Department of Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou, China.
authors.forename: Jian Ming
authors.initials: JM
authors.lastname: Wen
authors.forename: Jie Fu
authors.initials: JF
authors.lastname: Huang
authors.forename: Liang
authors.initials: L
authors.lastname: Hu
authors.forename: Wei Sheng
authors.initials: WS
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Jonathan S T
authors.initials: JS
authors.lastname: Sham
authors.forename: Jian Min
authors.initials: JM
authors.lastname: Xu
authors.forename: Wei Fen
authors.initials: WF
authors.lastname: Zeng
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.forename: Li Jian
authors.initials: LJ
authors.lastname: Liang
authors.forename: Xin Yuan
authors.initials: XY
authors.lastname: Guan
mesh_terms: D000782:Aneuploidy; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology / Q000556:secondary*; D002871:Chromosome Banding; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics / Q000648:ultrastructure; D005784:Gene Amplification; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D011169:Portal Vein; D014407:Tumor Cells, Cultured; D019043:Vascular Neoplasms / Q000235:genetics / Q000473:pathology / Q000556:secondary*