title: cDNA macroarray analysis of gene expression in synoviocytes stimulated with TNFalpha.
abstract: Gene expression of synoviocytes stimulated with tumor necrosis factor-alpha (TNFalpha) was studied by macroarray analysis to elucidate the cellular response and identify new biological functions of known and unknown genes. 10035 cDNA clones were used to make cDNA macroarrays of representative genes. Synoviocytes expressed large amounts of fibronectin and collagen mRNA. Statistical analysis of the macroarray data revealed 26 genes, including six new genes, which underwent significant alteration of gene expression in response to TNFalpha stimulation. These findings suggest that the synoviocyte response to TNFalpha stimulation forms the basis of development of various aspects of the pathophysiology of rheumatoid arthritis.
authors.affiliation: Helix Research Institute, Inc., 1532-3 Yana, Kisarazu-shi, Chiba, Japan. tsugiyama@kyowa.co.jp
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Shizuko
authors.initials: S
authors.lastname: Ishii
authors.forename: Jun ichi
authors.initials: Ji
authors.lastname: Yamamoto
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Irie
authors.forename: Kaoru
authors.initials: K
authors.lastname: Saito
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Otuki
authors.forename: Ai
authors.initials: A
authors.lastname: Wakamatsu
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Yuri
authors.initials: Y
authors.lastname: Hio
authors.forename: Toshio
authors.initials: T
authors.lastname: Ota
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nishikawa
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Masuho
authors.forename: Takao
authors.initials: T
authors.lastname: Isogai
mesh_terms: D000595:Amino Acid Sequence; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D017386:Sequence Homology, Amino Acid; D013583:Synovial Membrane / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D005353:Fibronectins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D003094:Collagen