An automated LC method for the small-scale purification of organic molecules derived from combinatorial libraries.
This paper presented the development of an automated HPLC small-scale purification method for single bead compounds derived from combinatorial libraries. The method was found to produce higher and more consistent recoveries of purified compounds as compared to conventional manual HPLC purification. Using the manual method, the average percentage recovery of one synthetic compound was determined to be 24% and the coefficients of variation (C.V.%) of recovery were found to be greater than 38%. Using the automated system, the average percentages recovery of a standard compound at 600 and 1000 micromol l(-1) were determined to be 72.63+/-10.17% and 81.34+/-4.39%, respectively. This represented an approximate 3-folds increase in percentage recovery compared to that of the manual small-scale purification process. It was also found that the C.V.% of recovery were less than 15% at both concentration levels. The development of this automated method was found to be straightforward. The importance and implications of this study were discussed.
Chan
Eric C Y
EC
Analytical Chemistry, Chemistry Discovery, S*B10 Pte Ltd, 1 Science Park Road, #05-09, The Capricon, Singapore Science Park II, Singapore 117528, Singapore. eric_chan@sbio.com
Wang
Alice W
AW
Jia
W P
WP
Dollinger
Gavin D
GD
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D013678:Technology, Pharmaceutical
D004364:Pharmaceutical Preparations