Introduction of functional groups onto polypropylene and polyethylene surfaces for immobilization of enzymes.
Polypropylene and polyethylene surfaces are activated by introducing an active functional group through 1-fluoro-2 nitro-4-azidobenzene by UV irradiation. Horseradish peroxidase and glucose oxidase are immobilized onto the activated surfaces, simply by incubating the enzymes at 37 degrees C. When untreated surfaces are used, insignificant immobilization of the enzymes is observed.
Naqvi
Azmi
A
Centre for Biochemical Technology (CSIR), Delhi University Campus, Mall Road, Delhi, 110007, India.
Nahar
Pradip
P
Gandhi
R P
RP
D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D011095:Polyethylenes / Q000737:chemistry*; D011126:Polypropylenes / Q000737:chemistry*; D011485:Protein Binding; D013499:Surface Properties
D004800:Enzymes, Immobilized; D011095:Polyethylenes; D011126:Polypropylenes; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase