Identification of a splice variant of mouse caveolin-2 mRNA encoding an isoform lacking the C-terminal domain.
We identified a splice variant of mouse caveolin-2 mRNA having an intronic sequence in place of the third exon (Deltaex3). The entire sequence of full-length (FL) and Deltaex3 caveolin-2 mRNA was determined; their sizes were 2490 and 973 bp, respectively. The Deltaex3 mRNA encoded a putative isoform lacking the C-terminal 49 amino acids of the authentic caveolin-2. The expression level of Deltaex3 was lower than that of FL mRNA, but considerable in some culture cells and tissues. The isoform lacking the C-terminus localized to the endoplasmic reticulum, while the authentic caveolin-2 was distributed to the Golgi and the plasma membrane along with caveolin-1. The result confirmed the necessity of the C-terminal domain of caveolin-2 for the caveolar localization, and showed the existence of a novel caveolin-2 isoform, which is not recruited to caveolae even in the presence of caveolin-1.
Kogo
Hiroshi
H
Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Showa-ku, 466-8550, Japan. hkogo@med.nagoya-u.ac.jp
Ishiguro
Kimiko
K
Kuwaki
Susumu
S
Fujimoto
Toyoshi
T
D016475:3T3 Cells; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D021941:Caveolae / Q000378:metabolism; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005091:Exons; D006056:Golgi Apparatus / Q000378:metabolism; D007438:Introns; D008297:Male; D051379:Mice; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics*; D014018:Tissue Distribution
C495541:Cav1 protein, mouse; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins; D012333:RNA, Messenger