Isolation and identification of antioxidants from Sophora japonica.
A new flavonol triglycoside, kaempferol 3-O-alpha-L-rhamnopyranosyl-(1 --> 6)-beta-D-glucopyranosyl-(1 --> 2)-beta-D-glucopyranoside (1), as well as two known kaempferol 3-O-[alpha-L-rhamnopyranosyl-(1 --> 6)]-[beta-D-glucopyranosyl-(1 --> 2)]-beta-D-glucopyranoside (2) and kaempferol 3-O-beta-D-glucopyranosyl-(1 --> 2)-beta-D-glucopyranoside-7-O-alpha-L-rhamnopyranoside (3), were isolated from the n-BuOH extract of the pericarps of Sophora japonica by bioassay-guided fractionation. The structure of compound 1 was established by UV, IR, MS, and one- and two-dimensional NMR spectroscopy, including DEPT, NOESY, DQF-COSY, TOCSY, HMQC, and HMBC experiments. Compounds 1-3 showed antioxidative activity in DPPH and cytochrome-c assay using HL-60 cell system.
Tang
Yu-Ping
YP
Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing. yptang@pub.sioc.ac.cn
Li
Yan-Fang
YF
Hu
Jie
J
Lou
Feng-Chang
FC
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001681:Biological Assay; D003574:Cytochrome c Group / Q000187:drug effects; D013058:Mass Spectrometry; D010946:Plants, Medicinal / Q000737:chemistry*; D029909:Sophora / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D003574:Cytochrome c Group