Trypanosoma cruzi reinfections in mice determine the severity of cardiac damage.
In two murine models we studied Trypanosoma cruzi reinfection in the acute and chronic phase of experimental Chagas' disease in order to elucidate the relevance of reinfections in determining the variability of cardiac symptoms and the irreversible cardiac damage. They were followed for 120 and 600 days post infection (p.i.) for the acute and chronic model, respectively. Reinfected mice reached higher parasitaemia levels than infected mice. The survival was 33 and 21% in the chronic phase for mice reinfected in the acute phase and 13% for mice reinfected in the chronic stage at the end of the experiments. Sixty-six percent of the infected group presented electrocardiographic abnormalities (heart frequency, prolonged PQ segment or QRS complex) in the chronic stage whereas 100% of the reinfected animals exhibited electric cardiac dysfunction since 90 and 390 days p.i. for the acute and chronic reinfected model, respectively (P<0.01). Heart histopathological studies showed fibrosis and necrosis areas and mononuclear infiltrates supporting the view that parasite persistence is a major factor in continuing the tissue inflammation. This work shows that T. cruzi reinfections could be related to the variability and severity of the clinical course of Chagas' disease and that parasite persistence is involved in exacerbation of the disease.
Bustamante
Juan M
JM
Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa 1085, Argentina.
Rivarola
Héctor W
HW
Fernández
Alicia R
AR
Enders
Julio E
JE
Fretes
Ricardo
R
Palma
José A
JA
Paglini-Oliva
Patricia A
PA
D000818:Animals; D014355:Chagas Disease / Q000469:parasitology* / Q000473:pathology; D004195:Disease Models, Animal; D004562:Electrocardiography; D005260:Female; D006331:Heart Diseases / Q000469:parasitology* / Q000473:pathology; D006651:Histocytochemistry; D051379:Mice; D018512:Parasitemia / Q000473:pathology; D014349:Trypanosoma cruzi / Q000254:growth & development*