Reactions of alpha amylases with starch granules in aqueous suspension giving products in solution and in a minimum amount of water giving products inside the granule.
Porcine pancreatic alpha amylase (PPA) and Bacillus amyloliquefaciens alpha amylase (BAA) were allowed to react with starch granules from maize, waxy maize, amylomaize-7, and potato in an aqueous suspension with a starch to water ratio of 1:10 and in a minimum of water with a starch to water ratio of 1:1. Quantitative amounts of the maltodextrin products were determined by TLC and scanning densitometry. The two alpha amylases gave different products that were characteristic of their unique action patterns. The percent conversion differed for the different kinds of starches and for the two kinds of reaction conditions. Maize and waxy maize starches were converted into about twice as much maltodextrins than were amylomaize-7 and potato starches by both enzymes and under both reaction conditions. The aqueous suspension gave much greater conversion into maltodextrins than did the minimum water condition. BAA gave 3-14% greater conversion of the granules into maltodextrins than did PPA, with the exception of potato starch.
Yook
Cheol
C
Laboratory of Carbohydrate Chemistry and Enzymology, 4252 Molecular Biology Building, Iowa State University, Ames 50011, USA.
Robyt
John F
JF
D000818:Animals; D001407:Bacillus / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011198:Solanum tuberosum / Q000166:cytology / Q000378:metabolism; D012996:Solutions / Q000737:chemistry / Q000378:metabolism; D013213:Starch / Q000737:chemistry* / Q000378:metabolism*; D013535:Suspensions / Q000737:chemistry / Q000378:metabolism; D013552:Swine; D014867:Water / Q000378:metabolism*; D003313:Zea mays / Q000166:cytology / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism*
D011134:Polysaccharides; D012996:Solutions; D013535:Suspensions; D014867:Water; C008315:maltodextrin; D013213:Starch; D000516:alpha-Amylases