title: Expression of the RNA binding proteins, Mel-N1, Mel-N2, and Mel-N3 in adipose cells.
abstract: Mel-N1 (murine embryonic lethal abnormal vision [ELAV]), a mammalian homolog of Drosophila ELAV, is an mRNA binding protein of the RNA Recognition Motif family. Studies with the human homolog, Hel-N1 have supported the hypothesis that Hel-N1, and its splice variant, Hel-N2 play a role in mRNA metabolism. Thus it becomes logical to extend this hypothesis to the murine variant Mel-N1 which has been described as a neuronal protein with a minor level of expression in the testis. Our current work expands the potential function for this protein through demonstration of expression of the full-length message and splice variants in adipose tissue as well as preadipocyte and adipocyte cell lines.
authors.affiliation: Department of Biochemistry and Molecular Biology, The Brody School of Medicine, East Carolina University, Greenville, NC 27858, USA.
authors.forename: Chen
authors.initials: C
authors.lastname: Qi
authors.forename: James A
authors.initials: JA
authors.lastname: Wheeler
authors.forename: Ashlie
authors.initials: A
authors.lastname: Pruett
authors.forename: Phillip H
authors.initials: PH
authors.lastname: Pekala
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D015536:Down-Regulation; D051959:ELAV Proteins; D000067777:ELAV-Like Protein 2; D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009928:Organ Specificity; D011132:Polyribosomes / Q000378:metabolism; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D051959:ELAV Proteins; D000067777:ELAV-Like Protein 2; C086811:ELAVL2 protein, human; C099893:Elavl2 protein, mouse; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D016601:RNA-Binding Proteins