title: [Optimization of the medium and cultivation conditions of Penicillium roquefortii f39 producing the diketopiperazine alkaloid roquefortine].
abstract: We optimized the medium for cultivation of Penicillium roquefortii f39, a producer of roquefortine. In this medium, the roquefortine yield increased 1.5-2-fold. The increase in roquefortine content was associated with high biomass yield, but not with an increase in biosynthetic activity of the mycelium. Direct correlation was found between extracellular roquefortine concentration and amount of the inoculum. The introduction of sucrose into the growth medium allowed us to increase the concentration of roquefortine during fermentation to 90 mg/l.
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prosp. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.
authors.forename: D M
authors.initials: DM
authors.lastname: Boĭchenko
authors.forename: N F
authors.initials: NF
authors.lastname: Zelenkova
authors.forename: M U
authors.initials: MU
authors.lastname: Arinbasarov
authors.forename: T A
authors.initials: TA
authors.lastname: Reshetilova
mesh_terms: D003470:Culture Media; D004873:Ergolines / Q000378:metabolism*; D006576:Heterocyclic Compounds, 4 or More Rings; D007211:Indoles; D025282:Mycelium / Q000378:metabolism; D010407:Penicillium / Q000254:growth & development* / Q000378:metabolism; D010879:Piperazines; D013395:Sucrose
chemical_list: D003470:Culture Media; D004873:Ergolines; D006576:Heterocyclic Compounds, 4 or More Rings; D007211:Indoles; D010879:Piperazines; D013395:Sucrose; C012536:roquefortine
vernacular_title: Optimizatsiia sredy i usloviĭ kul'tivirovaniia Penicillium roquefortii f39--produtsenta diketopiperazinovogo alkaloida rokefortina.