[Localization of tick-borne encephalitis virus protein E antigenic determinant recognized by antihemagglutinating monoclonal antibodies using a phage-display peptide library].
Bacteriophages bearing peptides reacting with antihemagglutinating monoclonal antibodies (MAb) 10H10 to tick-borne encephalitis (TBE) virus protein E were selected from a phage-display peptide library by affinity selection and enzyme immunoassay. The library contained randomized peptides that are 6 amino acids long, fused with protein pIII and exposed on the surface of the bacteriophage. No significant homology between the sequences of selected peptides and TBE virus protein E was detected. Computer software was created to locate the conformation epitopes on the surface of protein E. Amino acids R73, C74, T76, M77, N103, C105, L107, and S112 were found to form a discontinuous epitope recognized by MAb 10H10. These amino acids are remote in the protein sequence but close in the tertiary structure and form a whole epitope located in the structural domain II of protein E. Presumably the localized amino acids bind to the cellular receptor for TBE virus.
Loktev
A V
AV
Kuvshinov
V N
VN
Melamed
N V
NV
Ivanisenko
V A
VA
Mishin
V P
VP
Il'ichev
A A
AA
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D001435:Bacteriophages / Q000235:genetics; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D000939:Epitopes / Q000032:analysis / Q000235:genetics / Q000276:immunology; D006389:Hemagglutinins, Viral / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D006389:Hemagglutinins, Viral; D019151:Peptide Library; D010455:Peptides; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus
Lokalizatsiia antigennoĭ determinanty belka E virusa kleshchevogo éntsefalita, uznavaemoĭ antigemaggliutiniruiushchimi monoklonal'nymi antitelami, s pomoshch'iu peptidnoĭ fagovoĭ biblioteki.