Chemokine receptor expression on MBP-reactive T cells: CXCR6 is a marker of IFNgamma-producing effector cells.
Cytokine-polarized T cells have distinct chemokine receptor (CKR) expression patterns associated with their cytokine secretion profiles. In order to investigate this paradigm in autoreactive human T cells, we have determined the CKR expression pattern of myelin basic protein (MBP)-reactive T cell lines (TCL) and compared these profiles to those of TCL-generated in response to tetanus toxoid (TT). Expression of CXCR6 and CXCR3 on TCL was significantly positively correlated with IFNgamma, and inversely correlated with IL-5 production. TT TCL had significantly higher expression of CCR7(-)/CD45RA(-) T effector memory (Tem) cells than MBP TCL. However, in MBP-specific TCL, CXCR6 was found to be the best marker of conversion to the Tem phenotype. CXCR6 and CXCR3 are likely to be important in the migration of effector memory T cells in Th1-mediated inflammatory diseases such as multiple sclerosis (MS).
Calabresi
Peter A
PA
Department of Neurology, School of Medicine, University of Maryland, Room 12-027, 655 W. Baltimore St., Baltimore, MD 21201, USA. pcala001@umaryland.edu
Yun
Sung Hae
SH
Allie
Rameeza
R
Whartenby
Katharine A
KA
D015415:Biomarkers; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D000939:Epitopes; D005434:Flow Cytometry; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis*; D015848:Interleukin-5 / Q000032:analysis; D009103:Multiple Sclerosis / Q000276:immunology; D004676:Myelin Basic Protein / Q000276:immunology*; D054367:Receptors, CXCR3; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine / Q000032:analysis; D018121:Receptors, Cytokine / Q000032:analysis* / Q000276:immunology; D043562:Receptors, G-Protein-Coupled; D011991:Receptors, Virus; D013601:T-Lymphocytes / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D018417:Th1 Cells / Q000737:chemistry / Q000276:immunology; D018418:Th2 Cells / Q000737:chemistry / Q000276:immunology
D015415:Biomarkers; C515187:CXCR3 protein, human; C106330:CXCR6 protein, human; D000939:Epitopes; D015848:Interleukin-5; D004676:Myelin Basic Protein; D054367:Receptors, CXCR3; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D043562:Receptors, G-Protein-Coupled; D011991:Receptors, Virus; D007371:Interferon-gamma