Base-catalyzed derivatization methodology for FA analysis. application to milk fat and celery seed lipid TAG.
In this paper, an alternative base-catalyzed methodology for the facile derivatization in mild conditions of lipid TAG prior to FA analysis is proposed. Reagents were prepared by proton exchange between potassium tert-butoxide and either ethanol, n-propanol, n-butanol, or 2-methoxyethanol and used for the synthesis, at 40 degrees C for 15 min, of the corresponding derivatives, which were directly analyzed by GC. This methodology can be used on a routine basis and has been applied to standard and complex natural lipid samples. Tripalmitin was used to determine optimal reaction conditions; and bovine milk fat, containing C4 to C22 acids, and celery (Apium graveolens) seed oil, characterized by a high level of petroselinic acid, were comparatively analyzed as their ethyl, n-propyl, n-butyl, and 2-methoxyethyl esters.
Destaillats
Frédéric
F
Department of Food Science and Nutrition, and Dairy Research Center (STELA), Université Laval, Sainte-Foy, Québec, Canada.
Angers
Paul
P
D000818:Animals; D000077102:Apium / Q000737:chemistry*; D002384:Catalysis; D002417:Cattle; D002849:Chromatography, Gas / Q000379:methods; D005227:Fatty Acids / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008892:Milk / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D014280:Triglycerides / Q000737:chemistry*
D005227:Fatty Acids; D010936:Plant Extracts; D014280:Triglycerides