title: Cytotoxic, hypoglycemic activity and phytochemical analysis of Rubus imperialis (Rosaceae).
abstract: Rubus imperialis, Artemia salina, 3-O-methylellagic-4'-O-alpha-rhamnose Acid Screening of different extracts, fractions and compounds from Rubus imperialis Chum. Schl. (Rosaceae) has been conduced using the brine shrimp microwell cytotoxicity assay. Three parts of the plant (methanolic extract from leaves, roots and stems), three fractions from roots (hexane, ethyl acetate and butanol) and three isolated compounds (niga-ichigoside F1, 23-hydroxytormentic acid, ellagic acid derivative) were tested. The most promising material (LC50 <1000 microg/ml) were the methanolic extract and ethyl acetate fraction from roots. However, there was little correlation observed in the degree of toxicities observed between the isolated compounds. On the other hand, the cytotoxicity and in vivo assays confirmed the hypoglycemic activity of methanolic extract and validated the Brazilian popular use of R. imperialis as an antidiabetic agent.
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
authors.forename: Márcia
authors.initials: M
authors.lastname: Kanegusuku
authors.forename: Jean Carlo
authors.initials: JC
authors.lastname: Benassi
authors.forename: Rozangela Curi
authors.initials: RC
authors.lastname: Pedrosa
authors.forename: Rosendo Augusto
authors.initials: RA
authors.lastname: Yunes
authors.forename: Valdir Cechinel
authors.initials: VC
authors.lastname: Filho
authors.forename: Alcíbia Azevedo
authors.initials: AA
authors.lastname: Maia
authors.forename: Márcia Maria
authors.initials: MM
authors.lastname: de Souza
authors.forename: Franco
authors.initials: F
authors.lastname: Delle Monache
authors.forename: Rivaldo
authors.initials: R
authors.lastname: Niero
mesh_terms: D000818:Animals; D001156:Artemia; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D001938:Brazil; D002470:Cell Survival / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D027824:Rosaceae / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010936:Plant Extracts