Rotational drag on DNA: a single molecule experiment.
Within a single-molecule configuration, we have studied rotational drag on double stranded linear DNA by measuring the force during mechanical opening and closing of the double helix at different rates. The molecule is cranked at one end by the effect of unzipping and is free to rotate at the other end. In this configuration the rotational friction torque tau on double-stranded DNA leads to an additional contribution to the opening force. It is shown that the effect of rotational drag increases with the length of the molecule, is approximately proportional to the angular velocity of cranking, and we estimate that the torque tau is of the order of 1k(B)T for 10 000 base pairs of DNA cranked at 2000 turns per second.
Thomen
Philippe
P
Laboratoire de Physique de la Matière Condensée, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France.
Bockelmann
Ulrich
U
Heslot
François
F
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000737:chemistry*; D017276:Friction; D009690:Nucleic Acid Conformation; D019415:Torque
D004247:DNA