Expression of G1 phase cyclins in human gastric cancer and gastric mucosa of first-degree relatives.
The aim of this study was to investigate the expression of D-type cyclins and cyclin E in gastric cancer patients (N = 34), in healthy first-degree relatives of gastric cancer patients (N = 29), and in control subjects (N = 18). Expression of cyclins D1, D2, D3, and E was determined by RT-PCR. Localization of cyclin expression was determined by immunohistochemistry. Expression of cyclins D2, D3, and E was more frequently detected in tumor tissue compared with tumor-free gastric mucosa (P < 0.05) and was associated with the presence of intestinal metaplasia. In contrast, cyclin D1 was frequently expressed in both tumor- and tumor-free tissue. Cyclin D3 expression was more frequently detected in the antrum mucosa of first-degree relatives compared to controls (P < 0.01) and was associated with the presence of Helicobacter pylori. Our data suggest that deregulation of G1 phase cyclins may play a role in gastric carcinogenesis, and may point to the presence of molecular alterations in individuals at an increased risk for gastric cancer.
Miehlke
Stephan
S
Medical Department I, Gastroenterology, Hematology & Oncology, Technical University Hospital, Dresden, Germany. miehlke@mk1.med.tu-dresden.de
Yu
Jun
J
Ebert
Matthias
M
Szokodi
Daniel
D
Vieth
Michael
M
Kuhlisch
Eberhard
E
Buchcik
Rene
R
Schimmin
Wolfgang
W
Wehrmann
Ursula
U
Malfertheiner
Peter
P
Ehninger
Gerhard
G
Bayerdörffer
Ekkehard
E
Stolte
Manfred
M
D000368:Aged; D000369:Aged, 80 and over; D019938:Cyclin D1 / Q000378:metabolism*; D019927:Cyclin E / Q000378:metabolism*; D005260:Female; D016193:G1 Phase / Q000502:physiology*; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism*
D019927:Cyclin E; D019938:Cyclin D1