In vitro cultivation of Chinese strains of Babesia spp.
Microaerophilus stationary phase culture techniques were applied for the continuous cultivation of two Chinese strains of Babesia bovis and B. bigemina, to determine the efficacy of antibabesial drugs. The efficacy of chloroquine phosphate was 25 times higher than that of berenil. Primaquine phosphate only partially reduced the parasitaemia. The results show that Chinese Babesia strains can be cultivated in vitro and that this culture system is useful for the screening of antibabesial drugs.
Wu
Jiansan
J
Animal Quarantine Institute, Ministry of Agriculture, Qingdao, China.
Yu
Jianmin
J
Wang
Zhiliang
Z
Zweygarth
Erich P
EP
D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D001403:Babesia / Q000187:drug effects* / Q000254:growth & development*; D016793:Babesia bovis / Q000187:drug effects* / Q000254:growth & development*; D003470:Culture Media; D006801:Humans; D021261:Parasitic Sensitivity Tests / Q000379:methods
D000981:Antiprotozoal Agents; D003470:Culture Media