Effect of the essential oil from the flowers of Magnolia sieboldii on the lipopolysaccharide-induced production of nitric oxide and prostaglandin E2 by rat peritoneal macrophages.
The essential oil from the flowers of Magnolia sieboldii was tested for its effects on lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E(2) (PGE(2)) by rat peritoneal macrophages. It was shown to induce the production of NO and PGE 2 in a concentration-dependent manner (3 - 30 microg/ml). Gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) led to the identification of sixty compounds, of which beta-elemene (18.0 %), alpha-terpinene (14.83 %) and beta-myrcene (12.72 %) were the major constituents. Among these three compounds, alpha-terpinene was found to be the most effective one with inhibitory activity on NO and PGE(2) production by LPS-stimulated rat peritoneal macrophages.
Lim
Soon Sung
SS
Shin
Kuk Hyun
KH
Ban
Hyun Seung
HS
Kim
Yong Pil
YP
Jung
Sang Hoon
SH
Kim
Yoo Jeung
YJ
Ohuchi
Kazuo
K
D000080462:Acyclic Monoterpenes; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D000081005:Cyclohexane Monoterpenes; D015232:Dinoprostone / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D029562:Magnoliaceae; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D039821:Monoterpenes; D009569:Nitric Oxide / Q000096:biosynthesis*; D009573:Nitrites / Q000378:metabolism; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D012717:Sesquiterpenes; D013729:Terpenes / Q000302:isolation & purification / Q000494:pharmacology
D000080462:Acyclic Monoterpenes; D000081005:Cyclohexane Monoterpenes; D008070:Lipopolysaccharides; D039821:Monoterpenes; D009573:Nitrites; D009822:Oils, Volatile; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; C445979:beta-elemene; C008574:beta-myrcene; D009569:Nitric Oxide; C018669:gamma-terpinene; D015232:Dinoprostone