Carbohydrate carriers affect adhesion of H. pylori to immobilized Leb-oligosaccharide.
The present study involved comparison of adhesion of Helicobacter pylori KH202 to immobilized Le(b)-oligosaccharide carried on different carriers, i.e. Leb-oligosaccharide conjugated with polyacrylamide, bovine serum albumin, and dipalmitoylphosphatidylethanolamine (Le(b)-PAA, Le(b)-BSA, and Le(b)-DPPE). All of the Le(b)-oligosaccharide-carrying neoglycoconjugates served as ligands for H. pylori. However, H. pylori required 10-fold and 100-fold quantities of Le(b)-antigen to adhere to Le(b)-PAA and to Le(b)-DPPE in comparison to the quantity of Le(b)-antigen needed to adhere to Le(b)-BSA, respectively. H. pylori adhesion to Le(b)-PAA and Le(b)-DPPE was clearly inhibited by Le(b)-oligosaccharide, but adhesion to Le(b)-BSA was hardly inhibited by the oligosaccharide. Therefore, the carbohydrate carrier affects the affinity of H. pylori KH202 toward Le(b)-antigen, although the bacteria recognize Le(b)-antigen regardless of the carbohydrate carrier.
Kojima
Naoya
N
Department of Applied Biochemistry, and Institute of Glycotechnology, Tokai University, Hiratsuka, Kanagawa, Japan. naoyaki@keyaki.cc.u-tokai.ac.jp
Seino
Keita
K
Sato
Yoshia
Y
Mizuochi
Tsuguo
T
D000180:Acrylic Resins / Q000737:chemistry; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D006001:Glycoconjugates / Q000737:chemistry / Q000378:metabolism*; D016480:Helicobacter pylori / Q000502:physiology*; D007983:Lewis Blood Group Antigens / Q000737:chemistry / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D000099166:Polyacrylamides
D000180:Acrylic Resins; D006001:Glycoconjugates; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D010714:Phosphatidylethanolamines; D012710:Serum Albumin, Bovine; D000099166:Polyacrylamides; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine