title: Preparation and characterization of Cryptococcus neoformans synchronous culture.
abstract: We have developed a method for preparation of synchronous culture in Cryptococcus neoformans. The method is based on age fractionation of exponentially growing asynchronous culture through differential sedimentation in 10-20% (w/v) lactose gradient. C. neoformans capsule thickness should be reduced to a minimum to ensure most accurate age fractionation, which is necessary to obtain a higher degree of synchrony. The C. neoformans synchronous culture system has revealed important characteristics with respect to cellular morphology, DNA content and cell volume distribution. The method can be used for further cell cycle studies.
authors.affiliation: Department of Biology, Faculty of Medicine, Palacky University, Hnevotinska 3, CZ-775 15 Olomouc, Czech Republic. rac@tunw.upol.cz
authors.forename: Vladislav
authors.initials: V
authors.lastname: Raclavsky
authors.forename: Misako
authors.initials: M
authors.lastname: Ohkusu
authors.forename: Pavla
authors.initials: P
authors.lastname: Hruskova
authors.forename: Kanji
authors.initials: K
authors.lastname: Takeo
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle; D003455:Cryptococcus neoformans / Q000254:growth & development*; D008856:Microscopy, Fluorescence; D009172:Mycology / Q000379:methods*