[Identification, mapping, and application of a chromosome-specific RAPD marker from Haynaldia villosa].
Random amplified polymorphic DNA (RAPD) analysis was performed on common wheat of Chinese Spring, addition lines of H. villosa chromosome in CS and H. villosum from different accessions with 100 random 10-base primers. A chromosome-specific polymorphic DNA segment for H. villosa, OPF02(750), was obtained from all addition lines of H. villosa chromosome in CS and H. villosum which belong to different accessions. The result amplified by primer OPF02 of all addition lines of H. villosa chromosome in CS indicated that all the seven pairs of H. villosa chromosomes contain OPF02(750) segment. There was no OPF02(750) in all Triticum aestivum and T. durum tested. Using OPF02, We confirmed that NAU302, an addition line of H. villosa chromosome 3V, had lost its chromosome 3V of H. villosa. Therefore, OPF02(750) is specific to chromosomes of H. villosa, and could be used as a molecular marker for detection of chromosome of H. villosa in wheat.
Liu
Shou-Bin
SB
College of Crop Sciences, China Agricultural University, Beijing 100094, China. liusb2000@263.net
Tang
Zhao-Hui
ZH
You
Ming-Shan
MS
Li
Bao-Yun
BY
Mao
Shan-Feng
SF
Song
Jian-Min
JM
Liu
Guang-Tian
GT
D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006109:Poaceae / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D014908:Triticum / Q000235:genetics
D018744:DNA, Plant; D005819:Genetic Markers