Cytochemical studies on histone-type and protamine-type proteins during spermiogenesis in Chara vulgaris and Chara tomentosa.
Comparative studies concerning detection of histone-type and protamine-type proteins were carried out on Chara species (C. vulgaris, C. tomentosa). Analysis of antheridia during spermiogenesis (stages I-X) of both Charta species showed very similar staining patterns obtained after reactions revealing the examined proteins. Cytochemical studies showed a replacement of lysine-rich histone proteins by more basic arginine-rich ones during medium spermiogenesis (st. VI-VIII) in two Charta species, while late spermiogenesis (st. IX) and mature spermatozoids (st. X) were characterised by the presence of protamine-like proteins only.
Popłońska
K
K
Department of Cytophysiology, University of Lódź, Poland. popkat@taxus.biol.uni.lodz.pl
D000818:Animals; D002843:Chromatin / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000378:metabolism*; D018521:Plant Physiological Phenomena; D011479:Protamines / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D016707:Tissue Fixation
D002843:Chromatin; D006657:Histones; D011479:Protamines