title: Identification of gene expression profile of dorsal root ganglion in the rat peripheral axotomy model of neuropathic pain.
abstract: Phenotypic modification of dorsal root ganglion (DRG) neurons represents an important mechanism underlying neuropathic pain. However, the nerve injury-induced molecular changes are not fully identified. To determine the molecular alterations in a broader way, we have carried out cDNA array on the genes mainly made from the cDNA libraries of lumbar DRGs of normal rats and of rats 14 days after peripheral axotomy. Of the 7,523 examined genes and expressed sequence tags (ESTs), the expression of 122 genes and 51 expressed sequence tags is strongly changed. These genes encompass a large number of members of distinct families, including neuropeptides, receptors, ion channels, signal transduction molecules, synaptic vesicle proteins, and others. Of particular interest is the up-regulation of gamma-aminobutyric acid(A) receptor alpha5 subunit, peripheral benzodiazepine receptor, nicotinic acetylcholine receptor alpha7 subunit, P2Y1 purinoceptor, Na(+) channel beta2 subunit, and L-type Ca(2+) channel alpha2delta-1 subunit. Our findings therefore reveal dynamic and complex changes in molecular diversity among DRG neurons after axotomy. Sequences reported in this paper have been deposited in the GenBank database (accession numbers BG 662484-BG 673712)
authors.affiliation: Laboratory of Sensory System, Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China.
authors.forename: Hua-Sheng
authors.initials: HS
authors.lastname: Xiao
authors.forename: Qiu-Hua
authors.initials: QH
authors.lastname: Huang
authors.forename: Fang-Xiong
authors.initials: FX
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Bao
authors.forename: Ying-Jin
authors.initials: YJ
authors.lastname: Lu
authors.forename: Chao
authors.initials: C
authors.lastname: Guo
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.forename: Wein-Jing
authors.initials: WJ
authors.lastname: Huang
authors.forename: Gang
authors.initials: G
authors.lastname: Fu
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: Xu
authors.forename: Xi-Ping
authors.initials: XP
authors.lastname: Cheng
authors.forename: Qing
authors.initials: Q
authors.lastname: Yan
authors.forename: Zhi-Dong
authors.initials: ZD
authors.lastname: Zhu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Ze-Guang
authors.initials: ZG
authors.lastname: Han
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019771:Axotomy; D004195:Disease Models, Animal; D020224:Expressed Sequence Tags; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000503:physiopathology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009443:Neuritis / Q000235:genetics / Q000503:physiopathology*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012584:Sciatic Nerve / Q000473:pathology / Q000502:physiology*