PPI1: a novel pathogen-induced basic region-leucine zipper (bZIP) transcription factor from pepper.
We have isolated a full-length cDNA, PPI1 (pepper-PMMV interaction 1), encoding a novel basic region-leucine zipper (bZIP) DNA-binding protein, from expressed sequence tags differentially expressed in Capsicum chinense P1257284 infected with Pepper mild mottle virus (PMMV). PPI1 encodes a predicted protein of 170 amino acids and contains a putative DNA-binding domain that shares significant amino acid identity with ACGT-binding domains of members of the bZIP DNA-binding protein family. PPI1 was localized in the nucleus and had transcriptional activation activity in yeast. Transcripts of the PPI1 gene were preferentially induced during an incompatible interaction by inoculation with PMMV, Pseudomonas syringae pv. syringae 61, and Xanthomonas campestris pv. vesicatoria race 3. However, the PPII gene was not induced by abiotic stressors that activate the plant defense-signaling pathway. Our data provide the first evidence that a bZIP transcription factor is preferentially induced by pathogen attack, suggesting that PPI1 may play a specific functional role in the regulation of expression of plant defense-related genes.
Lee
Sang Jik
SJ
Biotechnology Center, Nong Woo Bio Co, Ltd, Jeongdan, Ganam, Korea.
Lee
Mi Yeon
MY
Yi
So Young
SY
Oh
Sang Keun
SK
Choi
Soon Ho
SH
Her
Nam Han
NH
Choi
Doil
D
Min
Byung Whan
BW
Yang
Seung Gyun
SG
Harn
Chee Hark
CH
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D002212:Capsicum / Q000235:genetics* / Q000382:microbiology; D002467:Cell Nucleus / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins; D007113:Immunity, Innate / Q000235:genetics; D016350:Leucine Zippers / Q000235:genetics*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics; D016959:Xanthomonas campestris / Q000254:growth & development; D015003:Yeasts / Q000235:genetics
D018076:DNA, Complementary; D018744:DNA, Plant; D008164:Luminescent Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins