CD36 is differentially expressed by CD8+ splenic dendritic cells but is not required for cross-presentation in vivo.
Cross-presentation allows the processing of Ags from donor cells into the MHC class I presentation pathway of dendritic cells (DCs). This is important for the generation of cytotoxic T cell immunity and for induction of self tolerance. Apoptotic cells are reported to be efficient targets for cross-presentation, and in vitro studies using human DCs have implicated CD36 in their capture. In support of a role for CD36 in cross-presentation, we show that this molecule is differentially expressed by CD8(+) splenic DCs, which previously have been identified as responsible for cross-presentation in the mouse. Three different cross-presentation models were examined for their dependence on CD36. These included cross-priming to OVA-coated spleen cells and cross-tolerance to OVA transgenically expressed in the pancreatic islet beta cells under constitutive conditions or during beta cell destruction. In these models, CD36 knockout DCs were equivalent to wild-type DCs in their capacity to cross-present either foreign or self Ags, indicating that CD36 is not essential for cross-presentation of cellular Ags in vivo.
Belz
Gabrielle T
GT
Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, University of Melbourne, Parkville, Victoria, Australia.
Vremec
David
D
Febbraio
Maria
M
Corcoran
Lynn
L
Shortman
Ken
K
Carbone
Francis R
FR
Heath
William R
WR
D000818:Animals; D017951:Antigen Presentation; D001324:Autoantigens / Q000276:immunology / Q000378:metabolism; D018955:CD36 Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D016827:CD8 Antigens / Q000096:biosynthesis*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016923:Cell Death / Q000276:immunology; D002478:Cells, Cultured; D017637:Clonal Deletion / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D007275:Injections, Intravenous; D017710:Lymphocyte Transfusion; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology; D013154:Spleen / Q000166:cytology* / Q000276:immunology / Q000378:metabolism / Q000637:transplantation
D001324:Autoantigens; D018955:CD36 Antigens; D016827:CD8 Antigens; D010047:Ovalbumin