Simultaneous screening and detection of drugs in small blood samples and bloodstains.
A gas chromatography-mass spectrometry (GC-MS) method is described for the screening and detection of morphine, codeine, cocaine, benzoylecgonine, methylecgonine, cocaethylene, delta-9-tetrahydrocannabinol (THC), 11-nor-9-carboxy-THC (THC-COOH), 11-hydroxy-THC (11-OH-THC), amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymetamphetamine (MDMA) and N-methyl-1-(3,4-methylenedioxyphenyl)-2-butanamine (MBDB) in small blood samples and bloodstains using solid phase SPE columns and a pipetting robot (Gilson Aspec XL). The detection limits are in the order of 1.62-4.10 ng/50 microl spot (amphetamines), 0.15-0.82 ng/50 microl spot (cannabinoids), 1.67-4.70 ng/50 microl spot (cocaine and derivatives) and 4.53-4.91 ng/50 microl spot (opiates) and the correlation factors are between 0.9957 and 0.9999. The method has proven useful in forensic cases with only small sample volumes or bloodstains.
Schütz
Harald
H
Institute of Legal Medicine, University Giessen, Frankfurter Str 58, D-35392 Giessen, Germany. harald.f.schuetz@forens-med.uni-giessen.de
Gotta
Jan Christoph
JC
Erdmann
Freidoon
F
Risse
Manfred
M
Weiler
Günter
G
D000328:Adult; D000662:Amphetamines / Q000097:blood*; D001801:Blood Stains; D002186:Cannabinoids / Q000097:blood*; D005554:Forensic Medicine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D009294:Narcotics / Q000097:blood*; D011180:Postmortem Changes; D015813:Substance Abuse Detection / Q000379:methods*
D000662:Amphetamines; D002186:Cannabinoids; D009294:Narcotics