title: Eptastigmine, nicotinamide and nicotinic acid determination using an inhibition enzyme sensor; application to pharmaceutical analysis.
abstract: An enzyme inhibition biosensor, developed in our laboratory and previously used for the analysis of compounds with anticholinesterase activity (e.g. physostigmine, neostigmine, pyridostigmine nicotine and organophosphorus compounds) has now been tested for the analysis of another recently synthesized cholinesterase inhibitor, i.e. eptastigmine. In addition nicotinic acid and nicotinamide, although displaying weaker inhibition properties, were also tested in pharmaceutical products using the same inhibition enzyme sensor. The biosensor consisted of a hydrogen peroxide amperometric electrode coupled to a functionalised nylon membrane chemically bonding both the enzymes butyrylcholinesterase and choline oxidase; a butyrylcholine standard solution in glycine buffer acted as substrate. The response of the system to all the inhibitors considered was characterised completely and the analysis of several pharmaceutical formulations containing nicotinamide or nicotinic acid was also performed.
authors.affiliation: Dipartimento di Chimica, Università di Roma La Sapienza, P.le Aldo Moro 5, 00185 Roma, Italy. luigi.campanella@uniromal.it
authors.forename: Luigi
authors.initials: L
authors.lastname: Campanella
authors.forename: Roberto
authors.initials: R
authors.lastname: Cocco
authors.forename: Gabriele
authors.initials: G
authors.lastname: Favero
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Sammartino
authors.forename: Mauro
authors.initials: M
authors.lastname: Tomassetti
mesh_terms: D001681:Biological Assay; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000494:pharmacology; D009525:Niacin / Q000032:analysis* / Q000494:pharmacology; D009536:Niacinamide / Q000032:analysis* / Q000494:pharmacology; D004364:Pharmaceutical Preparations; D010830:Physostigmine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000494:pharmacology; D014665:Vasodilator Agents / Q000032:analysis* / Q000494:pharmacology
chemical_list: D002800:Cholinesterase Inhibitors; D004364:Pharmaceutical Preparations; D014665:Vasodilator Agents; D009536:Niacinamide; D009525:Niacin; C052450:physostigmine heptyl; D010830:Physostigmine