title: Adhesion of lactic acid bacteria to caco-2 cells and their effect on cytokine secretion.
abstract: Cytokines secreted by human enterocytes play a critical role in mucosal and systemic immunity. Intestinal microorganisms can influence this secretion. In the present study, 30 strains of lactic acid bacteria were characterized for their adhesion to Caco-2 cells and their potential to stimulate proinflammatory cytokine secretion by this cell line. The bacteria adhered in a strain-dependent manner to Caco-2 cells. Contact with lactobacilli did not result in the production of IL-6 or IL-8. A slight IL-6 and IL-8 production by a Caco-2 cell was detected after exposure to 8 of the tested Bifidobacterium strains. No correlation was found between adhesion and cytokine induction among the bacteria tested. This indicates that lactic acid bacteria, even those with strong adhesive properties, are not very likely to trigger an inflammatory response in human enterocytes.
authors.affiliation: Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama, Kanagawa, Japan.
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Morita
authors.forename: Fang
authors.initials: F
authors.lastname: He
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Fuse
authors.forename: Arthur C
authors.initials: AC
authors.lastname: Ouwehand
authors.forename: Hideo
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Masataka
authors.initials: M
authors.lastname: Hosoda
authors.forename: Koko
authors.initials: K
authors.lastname: Mizumachi
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kurisaki
mesh_terms: D000328:Adult; D001422:Bacterial Adhesion / Q000502:physiology*; D018938:Caco-2 Cells; D016207:Cytokines / Q000378:metabolism*; D020895:Enterocytes / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D007223:Infant; D015850:Interleukin-6 / Q000096:biosynthesis / Q000378:metabolism; D016209:Interleukin-8 / Q000096:biosynthesis / Q000378:metabolism; D007778:Lactobacillus / Q000502:physiology*
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D016209:Interleukin-8