Sphingomonas sp. strain KA1, carrying a carbazole dioxygenase gene homologue, degrades chlorinated dibenzo-p-dioxins in soil.
Hybridization analysis showed that a newly isolated carbazole (CAR)-degrading bacterium Sphingomonas sp. strain KA1 did not possess the gene encoding the terminal oxygenase component (carAa) of CAR 1,9a-dioxygenase at high homology (more than 90% identity) to that of another CAR-degrader, Pseudomonas resinovorans strain CA10. However, PCR experiments using the primers for amplifying the internal fragment of the carAa gene (810 bp for strain CA10) showed that a PCR product of unexpected size (1100 bp) was amplified. Sequence analysis revealed that this DNA region contained the portion of two possible ORFs, which showed moderate homology to CarAa and CarBa from strain CA10 (61% and 40% identities at the amino acid level, respectively). Inoculation of strain KA1 into dioxin-contaminated model soil resulted in 96% and 70% degradation of 2-mono- and 2,3-dichlorinated dibenzo-p-dioxin, respectively, after 7-day incubation.
Habe
Hiroshi
H
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan.
Ashikawa
Yuji
Y
Saiki
Yuko
Y
Yoshida
Takako
T
Nojiri
Hideaki
H
Omori
Toshio
T
D001426:Bacterial Proteins; D015139:Blotting, Southern; D004147:Dioxins / Q000032:analysis / Q000378:metabolism*; D049308:Dioxygenases; D005798:Genes, Bacterial / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D020578:Sphingomonas / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification
D001426:Bacterial Proteins; D004147:Dioxins; D012336:RNA, Ribosomal, 16S; D012987:Soil; D010105:Oxygenases; D049308:Dioxygenases; C108434:carbazole 1,9a-dioxygenase