The hyperlipemic hamster - a model for testing the anti-atherogenic effect of amlodipine.
Male Golden Syrian hamsters were subjected to a hyperlipemic diet. At intervals ranging from 2 to 14 weeks, the animals were examined for changes in serum constituents and structural modifications of lesion-prone areas: the cardiac valves, coronary arteries and aortic arch. Serum was characterized by a gradual increase in cholesterol, triglycerides and a decrease in total peroxyl-radical trapping potential. The sequence of modifications of the endothelial cells, smooth muscle cells, and migrating plasma monocytes as well as of the extracellular matrix were established. Amlodipine treatment of hyperlipemic hamster was assessed. Amlodipine exhibited an athero-protective effect, acting as antioxidant, reducing the LDL uptake by the vessel wall and consequently, limiting the size and extent of lesioned areas. The hyperlipemic hamster is a reliable model to unravel the cellular alterations leading to atheroma formation, and for testing the effect of drugs in this process.
Sima
A
A
Institute of Cellular Biology and Pathology Nicolae Simionescu, Bucharest, Romania.
Stancu
C
C
Constantinescu
E
E
Ologeanu
L
L
Simionescu
M
M
D017311:Amlodipine / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000648:ultrastructure; D001161:Arteriosclerosis; D006224:Cricetinae; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D008647:Mesocricetus / Q000502:physiology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009130:Muscle, Smooth / Q000166:cytology / Q000648:ultrastructure; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism; D014665:Vasodilator Agents / Q000494:pharmacology
D008077:Lipoproteins, LDL; D014280:Triglycerides; D014665:Vasodilator Agents; D017311:Amlodipine