title: [HMG1 domains: the victims of circumstance].
abstract: The method of circular dichroism (CD) was used to compare DNA behavior during its interaction with linker histone H1 and with non-histone chromosomal protein HMG1 at different ionic strength and at different protein content in the system. The role of negatively charged C-terminal fragment of HMG1 was analyzed using recombinant protein HMG1-(A + B), which lacks the C terminal amino acid sequence. The psi-type CD spectra were common for DNA interaction with histone H1, but no spectra of this type were observed in HMG1-DNA systems even at high ionic strength. The CD spectrum of the truncated recombinant protein at high salt concentration somewhat resembled the psi-type spectrum. Two very intense positive bands were located near 215 nm and near 273 nm, and the whole CD spectrum was positive. The role of C-terminal tail of HMG1 in formation of the ordered DNA-protein complexes is discussed.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064 Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Chikhirzhina
authors.forename: A M
authors.initials: AM
authors.lastname: Polianichko
authors.forename: A N
authors.initials: AN
authors.lastname: Skvortsov
authors.forename: E I
authors.initials: EI
authors.lastname: Kostyleva
authors.forename: C
authors.initials: C
authors.lastname: Houssier
authors.forename: V I
authors.initials: VI
authors.lastname: Vorob'ev
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D024243:HMGB1 Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012996:Solutions
chemical_list: D024243:HMGB1 Protein; D006657:Histones; D011994:Recombinant Proteins; D012996:Solutions; D004247:DNA
vernacular_title: HMG1-domeny: zalozhniki obstoiatel'stv.