The von Willebrand factor propeptide (VWFpp) traffics an unrelated protein to storage.
The von Willebrand factor (VWF) propeptide (VWFpp) is critical for the targeting of VWF multimers to storage granules. VWFpp alone efficiently navigates the storage pathway in AtT-20 and endothelial cells and chaperones mature VWF multimers to storage granules when the two proteins are expressed in cis or in trans. To further define the role of VWFpp in granular sorting, we examined its ability to sort an unrelated protein, C3alpha into the regulated secretory pathway. Chimeric constructs of VWFpp and the alpha-chain of C3 were developed. The C3alpha protein expressed alone did not sort to granules in AtT-20 cells. The trans expression of C3alpha and VWFpp resulted in granular storage of VWFpp but no corresponding storage of C3alpha. When C3alpha is expressed as a single chain molecule with VWFpp that was rendered uncleavable by furin, C3alpha is re-routed to storage and is colocalized with VWFpp. The uncleavable protein was expressed in bovine aortic endothelial cells where it sorted to Weibel-Palade bodies, colocalized with bovine VWF, and was released when agonist stimulated. We now demonstrate that VWFpp re-routes a constitutively secreted protein to the regulated storage pathway. Furthermore, our studies suggest that the VWFpp storage signal is contained within amino acids 201 to 741.
Haberichter
Sandra L
SL
Blood Research Institute, The Blood Center of Southeastern Wisconsin, Milwaukee, USA.
Jozwiak
Mary A
MA
Rosenberg
Jonathan B
JB
Christopherson
Pamela A
PA
Montgomery
Robert R
RR
D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D015926:Complement C3a / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D051379:Mice; D010455:Peptides / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology; D021381:Protein Transport / Q000502:physiology*; D011506:Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D022142:Secretory Vesicles / Q000378:metabolism; D020302:Weibel-Palade Bodies / Q000378:metabolism; D014841:von Willebrand Factor / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
D010455:Peptides; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011506:Proteins; D011993:Recombinant Fusion Proteins; D014841:von Willebrand Factor; D015926:Complement C3a