Inhibition of proliferation and induction of apoptosis by tetrandrine in HepG2 cells.
Tetrandrine is a bisbenzylisoquinoline alkaloid derived from the root of Stephania tetrandra S. Moore, which has been reported to elicit in vitro cytotoxic effects on HeLa cells, and in vivo suppressive effects on mouse ascites tumors. In the present study, we examined the antiproliferative and apoptosis-inducing activity of tetrandrine in HepG2 cells, a human hepatoma cell line. Tetrandrine showed potent cytotoxic activity in HepG2 cells (IC(50)=9.0+/-1.0 micro M) following incubation for 48 h. Dose-dependent induction of apoptosis was observed by agarose gel electrophoresis and flow cytometric analysis. Treatment of HepG2 cells with tetrandrine resulted in the activation of caspase-3 protease, and subsequent proteolytic cleavage of poly(ADP-ribose) polymerase. These results suggest that tetrandrine is potentially useful as a chemotherapeutic/chemopreventive agent in hepatocellular carcinoma.
Yoo
Su-Mi
SM
College of Pharmacy and Medicinal Resources Research Center, Wonkwang University, Iksan, Jeonbuk, South Korea.
Oh
Seon-Hee
SH
Lee
Sang-Jun
SJ
Lee
Bang-Wool
BW
Ko
Won-Gil
WG
Moon
Chang-Kiu
CK
Lee
Byung-Hoon
BH
D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D044182:Benzylisoquinolines; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D006131:Growth Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000473:pathology
D000470:Alkaloids; D044182:Benzylisoquinolines; D004365:Drugs, Chinese Herbal; D006131:Growth Inhibitors; C009438:tetrandrine