title: Native transfer RNA catalyzes Diels-Alder reaction.
abstract: In this paper we show that transfer ribonucleic acids (tRNAs) catalyze the Diels-Alder cycloaddition reaction. A new DNA oxidative damage product, 6-furfuryladenine (kinetin) or its riboside (diene), was transformed with dimethyl acetylenedicarboxylate or maleic anhydride (dienophile). The reaction proceeds in the presence of tRNA at high pressure but not at ambient condition. If so tRNA in prebiotic conditions (RNA world) had at least two functions: catalytic and a carrier of genetic information. It means that tRNA at high pressure shows catalytic properties and is a true Diels-Alderase.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12, 61704 Poznan, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Mielcarek
authors.forename: Miroslawa Z
authors.initials: MZ
authors.lastname: Barciszewska
authors.forename: Piotr
authors.initials: P
authors.lastname: Sałanski
authors.forename: Maciej
authors.initials: M
authors.lastname: Stobiecki
authors.forename: Janusz
authors.initials: J
authors.lastname: Jurczak
authors.forename: Jan
authors.initials: J
authors.lastname: Barciszewski
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D005075:Biological Evolution; D002384:Catalysis; D004249:DNA Damage; D004591:Electrophoresis, Polyacrylamide Gel; D007701:Kinetin; D008299:Maleic Anhydrides; D008956:Models, Chemical; D018384:Oxidative Stress; D011312:Pressure; D012343:RNA, Transfer / Q000378:metabolism*; D015003:Yeasts
chemical_list: D008299:Maleic Anhydrides; D012343:RNA, Transfer; D000225:Adenine; D007701:Kinetin