Cysteine protease activity and histamine release from the human mast cell line HMC-1 stimulated by recombinant streptococcal pyrogenic exotoxin B/streptococcal cysteine protease.
We constructed the expression vector pSK-SCP containing the streptococcal exotoxin B gene (spe b) which expressed protease activity. We showed that the recombinant streptococcal pyogenic exotoxin B/streptococcal cysteine protease (rSPE B/SCP) was secreted into the culture supernatant of the transformant and retained its SCP activity, which was equivalent to or greater than that of the naturally occurring molecule. The secreted rSPE B/SCP induced histamine release and degranulation of the human mast cell line HMC-1. This study may contribute to the understanding of the pathogenic role of SPE B/SCP in streptococcal infection and streptococcal toxic shock syndrome.
Watanabe
Yukino
Y
Department of Immunology and Infectious Diseases, Institute of Gerontology, Nippon Medical School, Nakahara-ku, Kawasaki 211-8533, Japan.
Todome
Yuko
Y
Ohkuni
Hisashi
H
Sakurada
Shinsaku
S
Ishikawa
Toshio
T
Yutsudo
Takashi
T
Fischetti
Vincent A
VA
Zabriskie
John B
JB
D001426:Bacterial Proteins; D001483:Base Sequence; D002460:Cell Line; D004269:DNA, Bacterial; D005098:Exotoxins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D006632:Histamine / Q000378:metabolism*; D006636:Histamine Release; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005098:Exotoxins; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin; D006632:Histamine