Expression of Colonization Factor Antigen I of Enterotoxigenic Escherichia coli in Shigella flexneri 2a T32.
A host-plasmid lethal balancing system was constructed based on asd gene in an avirulent strain of S.flexneri to express colonization factor antigen I(CFA-I) of enterotoxigenic Escherichia coli.The results of Western blotting suggested that avirulant strain of S.flexneri Fwl01 expressed CFA-I steadily. Examination of negatively-stained preparation of cultures by electron microscopy showed that S.flexneri Fwl01 carrying the plasmid pZHY21 had thick pili on its surface. Antibodies against CFA-I were decteted in sera of mice immunized with recombinant bacteria either orogastrically (o.g.) or intranasally(i.n.); simultaneously sIgA against CFA-I was also decteted in the intestine. This work is helpful for constructing multivalent reombinant vaccine for prevention of bacterial diarrhea.
Zhang
Bo
B
Beijing Institute of Biotechnology, Beijing 100071, China. zhangzs@nic.bmi.ac.cn
Li
Shu-Qin
SQ
Chen
Guan-Jun
GJ
Huang
Cui-Fen
CF
Zhang
Zhao-Shan
ZS