title: Effective synthetic peptide vaccine for foot-and-mouth disease in swine.
abstract: We have designed a peptide-based vaccine for foot-and-mouth disease (FMD) effective in swine. The peptide immunogen has a G-H loop domain from the VP1 capsid protein of foot-and-mouth disease virus (FMDV) and a novel promiscuous T helper (Th) site for broad immunogenicity in multiple species. The G-H loop VP1 site was optimised for cross-reactivity to FMDV by the inclusion into the peptide of cyclic constraint and adjoining sequences. The incorporation of consensus residues into the hypervariable positions of the VP1 site provided for broad immunogenicity. The vaccine protected 20 out of 21 immunised pigs from infectious challenge by FMDV O1 Taiwan using peptide doses as low as 12.5 microg, and a mild adjuvant that caused no lesions. A safe chemically-defined product would have considerable advantages for vaccination against FMD.
authors.affiliation: United Biomedical Inc., 25 Davids Drive, Hauppauge, NY 11788, USA. cywang@unitedbiomedical.com
authors.forename: Chang Yi
authors.initials: CY
authors.lastname: Wang
authors.forename: Tseng Yuan
authors.initials: TY
authors.lastname: Chang
authors.forename: Alan M
authors.initials: AM
authors.lastname: Walfield
authors.forename: John
authors.initials: J
authors.lastname: Ye
authors.forename: Ming
authors.initials: M
authors.lastname: Shen
authors.forename: Shih Ping
authors.initials: SP
authors.lastname: Chen
authors.forename: Ming Chang
authors.initials: MC
authors.lastname: Li
authors.forename: Yeou Liang
authors.initials: YL
authors.lastname: Lin
authors.forename: Ming Hwa
authors.initials: MH
authors.lastname: Jong
authors.forename: Ping Cheng
authors.initials: PC
authors.lastname: Yang
authors.forename: Nancy
authors.initials: N
authors.lastname: Chyr
authors.forename: Ed
authors.initials: E
authors.lastname: Kramer
authors.forename: Fred
authors.initials: F
authors.lastname: Brown
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D005536:Foot-and-Mouth Disease / Q000517:prevention & control*; D006168:Guinea Pigs; D008297:Male; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology / Q000592:standards*; D013552:Swine; D014765:Viral Vaccines / Q000276:immunology / Q000592:standards*
chemical_list: D036022:Capsid Proteins; D010455:Peptides; C442501:VP1 protein, Foot-and-mouth disease virus; D014765:Viral Vaccines