title: Cytokine network of eutopic and ectopic endometrium in women with adenomyosis.
abstract: OBJECTIVE
  Recent studies showed the impairment of local cytokine balance in women with external endometriosis, but similar findings concerning direct production of cytokines by immunocompetent cells of women with adenomyosis are absent. In this context, investigation of the cytokine synthesis by mononuclear cells (MNCs) infiltrating eutopic and ectopic endometrium is of special interest.
  
  
  METHODS
  Concentration of interferon-gamma (IFNgamma), interferon-alpha (IFNalpha), tumour necrosis factor-alpha (TNFalpha), interleukin- 1beta (IL-1beta) and epidermal growth factor (EGF) in supernatants (SNs) of 24-hr cultures of MNCs obtained from eutopic and ectopic endometrium of women with adenomyosis was determined by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The levels of IFNgamma, IFNalpha, TNFalpha, IL-1beta and EGF in SNs of eutopic endometrial MNCs of women with adenomyosis were significantly increased and the content of IL-8 in SNs was reduced compared with that of the control figures. Ectopic MNCs of women with adenomyosis produced higher levels of IFNgamma, IFNalpha and TNFalpha than the MNCs of normal endometrium. The production of IL-1beta, IL-8 and EGF by ectopic endometrial MNCs was significantly reduced.
  
  
  CONCLUSIONS
  The results obtained indicate a significant role of local cytokine production impairment in the development of adenomyosis.
authors.affiliation: Laboratory of Clinical Immunology, Research Institute of Maternity and Childhood, Ivanovo, Russia. sotnikova@imail.ru
authors.forename: Natalia
authors.initials: N
authors.lastname: Sotnikova
authors.forename: Ioulia
authors.initials: I
authors.lastname: Antsiferova
authors.forename: Anna
authors.initials: A
authors.lastname: Malyshkina
mesh_terms: D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism*; D004715:Endometriosis / Q000276:immunology* / Q000473:pathology; D004717:Endometrium / Q000276:immunology* / Q000473:pathology; D004815:Epidermal Growth Factor / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D016898:Interferon-alpha; D007375:Interleukin-1; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D004815:Epidermal Growth Factor; D007371:Interferon-gamma