title: Ribozyme mediated suppression of vascular endothelial growth factor gene expression enhances matrix metalloproteinase 1 expression in a human hepatocellular carcinoma cell line.
abstract: The levels of expression of various genes were altered in cellular transformants with manipulation of expression of single genes. Vascular endothelial growth factor A (VEGF-A) is a key molecule for tumor progression, although it is unclear how VEGF-A expression regulates various genes. Multiple gene expression levels were evaluated using cDNA arrays in a human hepatocellular carcinoma cell line (HLF) with suppression of the VEGF-A gene by anti-VEGF-A ribozyme (alphaVRz). The ribozyme-mediated suppression of VEGF-A gene solely up-regulated matrix metalloproteinase 1 (MMP1) gene level in HLF/alphaVRz. Levels of expression of other members of MMP family or tissue inhibitors of MMPs did not show any alteration. These results suggested that intracellular suppression of VEGF-A gene was specifically linked to up-regulation of MMP1 in human hepatocellular carcinoma cells.
authors.affiliation: Department of Pathology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Junichiro
authors.initials: J
authors.lastname: Kamochi
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Tokunaga
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Morino
authors.forename: Junko
authors.initials: J
authors.lastname: Nagata
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tomii
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Abe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hatanaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kijima
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Norihito
authors.initials: N
authors.lastname: Watanabe
authors.forename: Shohei
authors.initials: S
authors.lastname: Matsuzaki
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Ueyama
authors.forename: Masato
authors.initials: M
authors.lastname: Nakamura
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000235:genetics; D016228:Endothelial Growth Factors / Q000037:antagonists & inhibitors / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology* / Q000235:genetics; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D016337:RNA, Catalytic / Q000494:pharmacology*; D014170:Transformation, Genetic; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A
chemical_list: D016228:Endothelial Growth Factors; D016337:RNA, Catalytic; D042461:Vascular Endothelial Growth Factor A; C415803:alpha VRz; D020781:Matrix Metalloproteinase 1