title: Leukopenia and thrombocytopenia in pigs after infection with bovine viral diarrhoea virus-2 (BVDV-2).
abstract: The aim of this study was to investigate whether bovine viral diarrhoea virus-2 (BVDV-2) is pathogenic for pigs, which organs become infected and whether or to which extent the virus is excreted into the environment. Ten pigs were observed for clinical reactions after infection with a BVDV-2 strain, that has been shown to be pathogenic in calves under experimental conditions. Samples were taken to monitor thrombocyte and leukocyte counts as well as antibody development. Post mortem examinations were performed at 7, 11 and 27 days after infection. Tissue samples were collected for virus isolation, histological and immunohistological examination. All ten pigs became infected and BVDV could be re-isolated from the lymphocytes, the plasma and different lymphatic organs. The infection passed clinically inapparent, apart from a slight increase in body temperature in some animals. Some animals developed a slight leukopenia and/or thrombocytopenia. There were no macroscopic or histological lesions observed that could specifically be related to the inoculation of BVDV-2. With respect to all parameters studied, the infection and the consequences thereof were clearly less pronounced in pigs as compared to cattle, the natural host. Our results indicate, that pigs infected with BVDV-2 might develop antibodies that cross-react in tests for antibodies against classical swine fever virus.
authors.affiliation: Department of Virological Research and Development, Intervet International BV, Boxmeer, The Netherlands. Birgit.Makoschey@Intervet.com
authors.forename: B
authors.initials: B
authors.lastname: Makoschey
authors.forename: E M
authors.initials: EM
authors.lastname: Liebler-Tenorio
authors.forename: Y M J C
authors.initials: YM
authors.lastname: Biermann
authors.forename: D
authors.initials: D
authors.lastname: Goovaerts
authors.forename: J F
authors.initials: JF
authors.lastname: Pohlenz
mesh_terms: D000818:Animals; D003429:Cross Reactions; D003937:Diagnosis, Differential; D030242:Diarrhea Virus 2, Bovine Viral / Q000302:isolation & purification / Q000472:pathogenicity*; D007970:Leukopenia / Q000209:etiology / Q000662:veterinary*; D018182:Pestivirus Infections / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000209:etiology* / Q000821:virology; D013921:Thrombocytopenia / Q000209:etiology / Q000662:veterinary*