title: Molecular diversity of the HLA-C gene identified in a caucasian population.
abstract: A DNA typing procedure, based on a two stage polymerase chain reaction-sequence-specific oligonucleotide probe (PCR-SSOP) typing strategy, has been developed and applied to DNA from 1000 healthy individuals from the Northern Ireland region. The two-stage procedure involves human leukocyte antigen (HLA-C) identification through the use of a medium resolution PCR-SSOP system, followed by four secondary group specific PCR-SSOP systems, to enable allele resolution. The PCR-SSOP systems were designed for the identification of HLA-Cw alleles with possible discrimination within exons 2 and 3 of the HLA-C gene, i.e., HLA-Cw*01-Cw*16. PCR-SSP tests were designed for the resolution of HLA-Cw*17 and -Cw*18 alleles. The systems can also be used independently of each other if selective allele resolution is required. HLA-Cw allele frequencies occurring within the Northern Ireland population have been compiled, along with estimations of HLA-B/Cw haplotype frequencies.
authors.affiliation: Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Northern, Belfast, Ireland. fionnuala.williams@bll.n-i.nhs.uk
authors.forename: Fionnuala
authors.initials: F
authors.lastname: Williams
authors.forename: Ashley
authors.initials: A
authors.lastname: Meenagh
authors.forename: Chris
authors.initials: C
authors.lastname: Patterson
authors.forename: Derek
authors.initials: D
authors.lastname: Middleton
mesh_terms: D014644:Genetic Variation; D015236:HLA-C Antigens / Q000145:classification / Q000235:genetics*; D006801:Humans; D044465:White People / Q000235:genetics*
chemical_list: D015236:HLA-C Antigens