title: High-throughput protein arrays: prospects for molecular diagnostics.
abstract: High-throughput protein arrays allow the miniaturized and parallel analysis of large numbers of diagnostic markers in complex samples. Using automated colony picking and gridding, cDNA or antibody libraries can be expressed and screened as clone arrays. Protein microarrays are constructed from recombinantly expressed, purified, and yet functional proteins, entailing a range of optimized expression systems. Antibody microarrays are becoming a robust format for expression profiling of whole genomes. Alternative systems, such as aptamer, PROfusion, nano- and microfluidic arrays are all at proof-of-concept stage. Differential protein profiles have been used as molecular diagnostics for cancer and autoimmune diseases and might ultimately be applied to screening of high-risk and general populations.
authors.affiliation: Biorchard Ltd., c/o Cornupia Capital Ltd., 27 Old Gloucester Street, London, UK WC1N 3XX. gerald@biorchard.com
authors.forename: Gerald
authors.initials: G
authors.lastname: Walter
authors.forename: Konrad
authors.initials: K
authors.lastname: Büssow
authors.forename: Angelika
authors.initials: A
authors.lastname: Lueking
authors.forename: Jörn
authors.initials: J
authors.lastname: Glökler
mesh_terms: D000818:Animals; D000906:Antibodies; D020869:Gene Expression Profiling; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D036103:Nanotechnology / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D019151:Peptide Library; D040901:Proteomics / Q000379:methods*
chemical_list: D000906:Antibodies; D019151:Peptide Library