Polymer hydrogels usable for nervous tissue repair.
The implantation of non-resorbable biocompatible polymer hydrogels into defects in the central nervous system can reduce glial scar formation, bridge the lesion and lead to tissue regeneration within the hydrogel. We implanted hydrogels based on crosslinked poly hydroxyethyl-methacrylate (pHEMA) and poly N-(2-hydroxypropyl)-methacrylamide (pHPMA) into the rat cortex and evaluated the cellular invasion into the hydrogels by means of immunohistochemical methods and tetramethylammonium diffusion measurements. Astrocytes and NF160-positive axons grew similarly into both types of hydrogels. We found no cell types other than astrocytes in the pHEMA hydrogels. In the pHPMA hydrogels, we found a massive ingrowth of connective tissue elements. These changes were accompanied by corresponding changes in the extracellular space volume fraction and tortuosity of the hydrogels.
Lesný
P
P
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20, Prague, Czech Republic.
De Croos
J
J
Prádný
M
M
Vacík
J
J
Michálek
J
J
Woerly
S
S
Syková
E
E
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000293:injuries / Q000502:physiology*; D003238:Connective Tissue / Q000187:drug effects / Q000502:physiology; D004353:Drug Evaluation, Preclinical; D004952:Esters / Q000494:pharmacology; D020100:Hydrogels / Q000494:pharmacology*; D008689:Methacrylates / Q000494:pharmacology; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology*; D011102:Polyhydroxyethyl Methacrylate / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D001672:Biocompatible Materials; D004952:Esters; D020100:Hydrogels; D008689:Methacrylates; D011108:Polymers; C447720:methacryloylglycylglycine-4-nitrophenyl ester; D011102:Polyhydroxyethyl Methacrylate