Effect of Misgurnus anguillicaudatus polysaccharide on immune responses of splenocytes in mice.
OBJECTIVE
To investigate the effect of Misgurnus anguillicaudatus polysaccharides (MAP) on immune responses of splenocytes in mice.


METHODS
T lymphocyte proliferation (TLP) was measured by [3H]thymidine incorporation assay. Cytotoxic T lymphocyte (CTL) cytotoxicity and natural killer (NK) activity were determined by release of radioactive chromium [51Cr] from pre-labeled target cells.


RESULTS
MAP 5 and 10 mg . kg-1 . d-1, ip for 7 d, could increase TLP, enhance the cytotoxicity of CTL and NK cells, and antagonize the effect of concanavalin A (ConA) on TLP suppressed by cyclophosphamide (CP). Inhibitory rates of CTL cytotoxicity were decreased from 51.4 % in CP control mice to 18.2 % and 35.1 % in MAP-treated CP mice, respectively. Furthermore, administration of MAP 10 and 20 mg . kg-1 . d-1, ip for 7 d, restored the reduced NK cell cytotoxicity caused by CP administration in mice.


CONCLUSIONS
MAP has protective effect on augmenting T-cell-mediated immunity and NK activity in normal and CP-treated mice.
Qin
Chuan-Guang
CG
Pharmaceutical Institute, Huazhong University of Science and Technology, Wuhan 430074, China.
Huang
Kai-Xun
KX
Xu
Hui-Bi
HB
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003520:Cyclophosphamide; D003531:Cypriniformes; D016867:Immunocompromised Host; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D051379:Mice; D011134:Polysaccharides / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology
D000276:Adjuvants, Immunologic; D011134:Polysaccharides; D003520:Cyclophosphamide