title: Conservation of a dinucleotide simple sequence repeat locus in sharks.
abstract: Recent studies indicate that the flanking region and repeat motif structure of conserved microsatellite loci are useful for phylogenetic inference. Most comparative studies of microsatellite loci involve relatively closely related species, however, primarily because primers developed for one species often amplify only related species. We describe an analysis of a microsatellite locus in lamniform sharks that we estimate has been conserved for a billion years. Combined analysis of the flanking sequence and repeat motif structure resulted in a gene tree comparable to those reported from similar analyses of other genes. The conservation of the simple sequence repeat (SSR), and of the sequence flanking the SSR, is explained by a low substitution rate in sharks coupled with the possibility that mutations which interrupt perfect repeats are lost by replication slippage.
authors.affiliation: Department of Environmental, Population, and Organismic Biology, University of Colorado, Boulder, CO 80309, USA. am@stripe.colorado.edu
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Martin
authors.forename: Amanda T
authors.initials: AT
authors.lastname: Pardini
authors.forename: Leslie R
authors.initials: LR
authors.lastname: Noble
authors.forename: Catherine S
authors.initials: CS
authors.lastname: Jones
mesh_terms: D000818:Animals; D017124:Conserved Sequence / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018900:Dinucleotide Repeats / Q000235:genetics*; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D017354:Point Mutation; D017422:Sequence Analysis, DNA; D012754:Sharks / Q000145:classification / Q000235:genetics*
chemical_list: D004247:DNA