Microwave decondensation and codenaturation: a new methodology to maximize FISH data from donors with very low concentrations of sperm.
To increase analyzable sperm numbers when dealing with semen samples from donors with extremely low quantities of sperm, a microwave decondensation/codenaturation technique for fluorescence in situ hybridization (FISH) was developed and results were compared to those obtained from conventional FISH techniques. There was no significant difference between the frequencies of disomy and diploidy obtained using the two techniques.
Ko
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Genetics Department, Alberta Children's Hospital, Calgary, Alta, Canada.
Rademaker
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A
Martin
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R
D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008872:Microwaves; D009693:Nucleic Acid Hybridization; D009845:Oligospermia / Q000235:genetics*; D013076:Sperm Count