[Endothelin-1 and bradykinin in nasal lavage fluid of patients allergic to tree pollen].
Hay fever is one of the most frequent upper airway diseases. The nasal epithelium is considered to play and active role in the allergic inflammation through its capacity to synthesize and release a wide range of cytokines and mediators. One substance of potential relevance in this regard is endothelin (ET). We postulated that ET-1 could contribute to pathomechanism of seasonal allergic rhinitis. The aim of this study was to demonstrate release of this peptide and bradykinin into the nasal lavage fluid (NLF) of tree pollen allergic patients in response to allergen challenge. 16 subjects aged 25.5 +/- 3.3 years with clinical history of tree pollen allergic rhinitis participated in the study. Nasal allergen provocation with following lavage was performed in all subjects. Ten of them were hyposensitized preseasonally by allergic vaccine Allergovit (Allergopharma, Germany). Six patients received treatment with antihistamines, cromoglicates or steroids. Bradykinin and endothelin-1 in the nasal secretion were assayed before starting immunotherapy and early during tree pollen season. Increase in concentration of bradykinin in nasal lavage fluid from hyposensitized patients were significantly lower than from second group, 509.4 +/- 110.4 before 942.9 +/- 234.89 pg/ml after allergen provocation versus 417.8 +/- 100.4 and 2001.6 +/- 223.6 pg/ml (p < 0.05). We noticed lower concentration of ET-1 in NLF of hay fever patients, 17.5 +/- 3.9 versus 13.9 +/- 2.7 pmol/ml. We conclude that bradykinin and endothelin-1 could be involved in the pathogenesis of hay fever.
Gawlik
Radosław
R
Katedra i Klinika Chorób Wewnetrznych, Alergologii i Immunologii Klinicznej Sl. AM w Katowicach.
Rogala
Barbara
B
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D001920:Bradykinin / Q000032:analysis*; D001985:Bronchial Provocation Tests; D019332:Endothelin-1 / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D017817:Nasal Lavage Fluid / Q000737:chemistry*; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis* / Q000378:metabolism
D015415:Biomarkers; D019332:Endothelin-1; D001920:Bradykinin
Endotelina-1 i bradykinina w popłuczynach nosowych u chorych uczulonych na pyłki drzew.