title: Localization of indoleamine 2,3-dioxygenase and 4-hydroxynonenal in normal and pre-eclamptic placentae.
abstract: This study was undertaken to compare placental levels of 2,3-Dioxygenase (IDO), a free radical scavenger, and 4-Hydroxynonenal (4-HNE), a major by-product of lipid peroxidation, in normal and pre-eclamptic pregnancies. Placentae were collected at caesarean section from women with a term, normal singleton pregnancy (37-40 weeks' gestation, n=10) and women with a term singleton pregnancy complicated by pre-eclampsia (n=10). IDO and 4-HNE localization and intensity was studied by semi-quantitative immunohistochemistry and differences between groups were analysed using the Mann-Whitney U test. Immunostaining for IDO was located primarily in endothelial cell nuclei, with a reduced level of staining in the cytoplasm, in most capillaries from all placentae examined. A significantly higher level of IDO immunostaining was observed in normal placentae compared to pre-eclamptic placentae (P=0.008). 4-HNE was located mainly in the cytoplasm of syncytiotrophoblast cells of all placentae examined. There were no significant differences in the pattern or intensity of 4-HNE immunostaining levels between normal and pre-eclamptic pregnancies (P=0.684). Our IDO results support the hypothesis of decreased anti-oxidative capability in the placenta and the possibility of an ineffective compensatory mechanism against increased oxidative stress in the fetus.
authors.affiliation: Centre for Women's Health Research Department of Obstetrics & Gynaecology, Monash University, Clayton, Victoria, 3168, Australia.
authors.forename: D I S
authors.initials: DI
authors.lastname: Santoso
authors.forename: P
authors.initials: P
authors.lastname: Rogers
authors.forename: E M
authors.initials: EM
authors.lastname: Wallace
authors.forename: U
authors.initials: U
authors.lastname: Manuelpillai
authors.forename: D
authors.initials: D
authors.lastname: Walker
authors.forename: Sri Bekti
authors.initials: SB
authors.lastname: Subakir
mesh_terms: D000328:Adult; D000447:Aldehydes / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology; D049308:Dioxygenases; D004730:Endothelium, Vascular / Q000201:enzymology; D005260:Female; D005865:Gestational Age; D006801:Humans; D007124:Immunoenzyme Techniques; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D010105:Oxygenases / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology*; D011225:Pre-Eclampsia / Q000201:enzymology*; D011247:Pregnancy / Q000378:metabolism*
chemical_list: D000447:Aldehydes; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D010105:Oxygenases; D049308:Dioxygenases; C027576:4-hydroxy-2-nonenal