Neurogenic differentiation of murine and human adipose-derived stromal cells.
The identification of cells capable of neuronal differentiation has great potential for cellular therapies. We examined whether murine and human adipose-derived adult stem (ADAS) cells can be induced to undergo neuronal differentiation. We isolated ADAS cells from the adipose tissue of adult BalbC mice or from human liposuction tissue and induced neuronal differentiation with valproic acid, butylated hydroxyanisole, insulin, and hydrocortisone. As early as 1-3 h after neuronal induction, the phenotype of ADAS cells changed towards neuronal morphology. Following neuronal induction, muADAS cells displayed immunocytochemical staining for GFAP, nestin and NeuN and huADAS cells displayed staining for intermediate filament M, nestin, and NeuN. Following neuronal induction of murine and human ADAS cells, Western blot analysis confirmed GFAP, nestin, and NeuN protein expression. Pretreatment with EGF and basic FGF augmented the neuronal differentiation of huADAS cells. The neuronal differentiation of stromal cells from adipose tissue has broad biological and clinical implications.
Safford
Kristine M
KM
Department of Surgery, Division of Pediatric Surgery, Box 3815, Duke University Medical Center, Durham, NC 27710, USA.
Hicok
Kevin C
KC
Safford
Shawn D
SD
Halvorsen
Yuan-Di C
YD
Wilkison
William O
WO
Gimble
Jeffrey M
JM
Rice
Henry E
HE
D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000943:Antigens, Differentiation / Q000096:biosynthesis; D000975:Antioxidants / Q000494:pharmacology; D015153:Blotting, Western; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005434:Flow Cytometry; D018682:GABA Agents / Q000494:pharmacology; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007150:Immunohistochemistry; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D014635:Valproic Acid / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D000943:Antigens, Differentiation; D000975:Antioxidants; D018682:GABA Agents; D007328:Insulin; D016222:Fibroblast Growth Factor 2; D002083:Butylated Hydroxyanisole; D014635:Valproic Acid; D004815:Epidermal Growth Factor; D006854:Hydrocortisone