Comparison of automated haematology analysers for detection of apoptotic lymphocytes.
Automated haematology analysers can rapidly provide accurate blood cell counts and white blood cell differentials. In this study, we evaluated four different haematology analysers for the detection of apoptotic lymphocytes in peripheral blood: MAXM A/L Retic, H*2, Cell-Dyn 3500 and NE-8000. With the MAXM A/L Retic haematology analyser, the apoptotic lymphocyte cluster appeared below the original lymphocyte cluster on the volume/DF1, and to the right under the original lymphocyte cluster on the volume/DF2 scattergrams. With the H*2 haematology analyser, the apoptotic polymorphonuclear lymphocytes produced a higher lobularity index on the BASO channel. With the Cell-Dyn 3500 haematology analyser, the apoptotic lymphocyte cluster appeared to the right side of the original lymphocyte cluster on the 0D/10D scattergram and to the left side of the polymorphonuclear cluster on the 90D/10D scattergram. With the NE-8000 haematology analyser, the apoptotic lymphocyte cluster was not distinguishable. Thus, apoptotic lymphocytes are readily detected on scattergrams generated by selected haematology analysers.
Taga
K
K
Department of Pediatrics, Neagari Hospital, No-85, Oohama-Neagari, Nomigun, Ishikawa 929-0122, Japan. taga3@yacht.ocn.ne.jp
Sawaya
M
M
Yoshida
M
M
Kaneko
M
M
Okada
M
M
Taniho
M
M
D017209:Apoptosis; D001772:Blood Cell Count / Q000295:instrumentation / Q000379:methods; D016022:Case-Control Studies; D005434:Flow Cytometry / Q000295:instrumentation* / Q000592:standards; D006801:Humans; D007244:Infectious Mononucleosis / Q000097:blood; D018655:Lymphocyte Count / Q000295:instrumentation / Q000379:methods; D008214:Lymphocytes / Q000473:pathology*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity