title: Assessment of induced corneal hypoxia in diabetic patients.
abstract: OBJECTIVE
  To assess the control of corneal hydration in patients with diabetes during a contact lens-induced hypoxia.
  
  
  METHODS
  Corneal stress was induced in 15 patients with diabetes and 23 healthy patients by having them wear contact lenses for 2 hours. Pachymetries were measured and corneal parameters (percentage recovery per hour [PRPH], time for deswelling [T99%] and induced swelling [IS]) were calculated. In the mean time, tears were collected to assess the activity of lactate dehydrogenase (LDH), and a specular microscopy (SM) was performed.
  
  
  RESULTS
  In patients with diabetes, PRPH, T99%, and LDH activity were statistically significantly modified (p < 0.05), whereas IS and SM were not.
  
  
  CONCLUSIONS
  This decreased ability to recover the initial corneal thickness after a transient edema caused by hypoxia confirms the enzymatic dysfunction of the endothelial pumps that are partly caused by a shift toward anaerobic metabolism.
authors.affiliation: Department of Ophthalmology, CHU, Hôpital Général, University of Burgundy, 3 Rue du Faubourg Raines, BP 1519, 21033 Dijon, France.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Ziadi
authors.forename: Philippe
authors.initials: P
authors.lastname: Moiroux
authors.forename: Philippe
authors.initials: P
authors.lastname: d'Athis
authors.forename: Alain
authors.initials: A
authors.lastname: Bron
authors.forename: Jean-Marcel
authors.initials: JM
authors.lastname: Brun
authors.forename: Catherine
authors.initials: C
authors.lastname: Creuzot-Garcher
mesh_terms: D000328:Adult; D000368:Aged; D002452:Cell Count; D003263:Contact Lenses, Hydrophilic / Q000009:adverse effects; D003315:Cornea / Q000201:enzymology / Q000503:physiopathology*; D015715:Corneal Edema / Q000201:enzymology / Q000209:etiology / Q000503:physiopathology*; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology / Q000503:physiopathology*; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology / Q000503:physiopathology*; D004728:Endothelium, Corneal / Q000473:pathology; D005260:Female; D006801:Humans; D000860:Hypoxia / Q000201:enzymology / Q000209:etiology / Q000503:physiopathology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013312:Stress, Physiological; D013666:Tears / Q000201:enzymology
chemical_list: D007770:L-Lactate Dehydrogenase