Inhibition of cell cycle progression in human leukemia HL-60 cells by esculetin.
Esculetin, a coumarin compound, was found to inhibit cell growth and cell cycle progression by inducing arrest of the G1 phase in HL-60 cells. To obtain information regarding cell cycle arrest induced by esculetin, we examined its effect on the regulating factors of the G1 phase in the leukemia HL-60 cells treated with esculetin by Western blotting. Our observations were: (1) a distinct increase in the level of hypophosphorylated retinoblastoma protein (pRb), and a reduction in the level of CDK4 after treatment with 100 microM of esculetin for 24 h; (2) a marked up-regulation of p27, and a down-regulation of cyclin D1 after treatment with 100 microM esculetin for 24 h. These results suggest that esculetin can inhibit the growth of human leukemia HL-60 cells by G1 phase cell cycle arrest as a result of inhibited pRb phosphorylation.
Wang
Chau Jong
CJ
Institute of Biochemistry, Chung Shan Medical University, Taichung, Taiwan.
Hsieh
Yu Jin
YJ
Chu
Chia Yih
CY
Lin
Yu Ling
YL
Tseng
Tsui Hwa
TH
D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D019938:Cyclin D1 / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D015536:Down-Regulation; D018922:HL-60 Cells; D006801:Humans; D008840:Microfilament Proteins / Q000378:metabolism; D009124:Muscle Proteins; D010766:Phosphorylation; D016160:Retinoblastoma Protein / Q000378:metabolism; D013997:Time Factors; D014468:Umbelliferones / Q000494:pharmacology*; D015854:Up-Regulation
D000970:Antineoplastic Agents; D000975:Antioxidants; D008840:Microfilament Proteins; D009124:Muscle Proteins; D016160:Retinoblastoma Protein; C058992:Tagln protein, mouse; D014468:Umbelliferones; D019938:Cyclin D1; D004247:DNA; C007628:esculetin