Differential expression of two somatostatin genes during zebrafish embryonic development.
We have identified the cDNAs of two new zebrafish preprosomatostatins, PPSS1 and PPSS3, in addition to the previously cloned PPSS2 (Argenton et al., 1999). PPSS1 is the orthologue of mammalian PPSSs, with a conserved C-terminal SS-14 sequence, PPSS2 is a divergent SS precursor and PPSS3 is a cortistatin-like prohormone. Using whole-mount in situ hybridisation, we have analysed the expression of PPSS1 and PPSS2 in zebrafish embryos up to 5 days post fertilisation. PPSS1 was expressed in the developing pancreas and central nervous system (CNS), whereas PPSS2 expression was exclusively pancreatic. In the CNS, PPSS1 was detected in several areas, in particular in the vagal motor nucleus and in cells that pioneer the tract of the postoptic commissure. PPSS1 was also expressed transiently in the telencephalon and spinal motor neurons. In all areas but the telencephalon PPSS1 was coexpressed with islet-1.
Devos
Nathalie
N
Laboratoire de Biologie Moléculaire et de Génie Génétique, Institut de Chimie, Bâtiment B6, Université de Liège, Belgium.
Deflorian
Gianluca
G
Biemar
Frédéric
F
Bortolussi
Marino
M
Martial
Joseph A
JA
Peers
Bernard
B
Argenton
Francesco
F
D000595:Amino Acid Sequence; D000818:Animals; D060466:Axin Protein; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism*; D018076:DNA, Complementary; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D010179:Pancreas / Q000196:embryology / Q000378:metabolism*; D010802:Phylogeny; D011498:Protein Precursors / Q000145:classification / Q000235:genetics*; D011506:Proteins / Q000235:genetics; D012097:Repressor Proteins; D017386:Sequence Homology, Amino Acid; D013004:Somatostatin / Q000145:classification / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D029961:Zebrafish Proteins
D060466:Axin Protein; D018076:DNA, Complementary; D011498:Protein Precursors; D011506:Proteins; D012097:Repressor Proteins; D029961:Zebrafish Proteins; C504314:cort protein, zebrafish; C504313:sst1.1 protein, zebrafish; D013004:Somatostatin