Synthesis of cyclosporin A-derived affinity reagents by olefin metathesis.
[reaction: see text] New affinity reagents were synthesized using alkene metathesis to directly modify the MeBmt side chain of cyclosporin A. The reagents were used to detect novel cyclophilins from cellular extracts.
Smulik
Jason A
JA
Department of Pharmacology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Diver
Steven T
ST
Pan
Fan
F
Liu
Jun O
JO
D000345:Affinity Labels / Q000138:chemical synthesis*; D000475:Alkenes / Q000737:chemistry*; D016572:Cyclosporine / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D007166:Immunosuppressive Agents / Q000737:chemistry*
D000345:Affinity Labels; D000475:Alkenes; D007166:Immunosuppressive Agents; D016572:Cyclosporine