Methods for detecting apoptosis in thyroid diseases.
Over the last few years, the importance of apoptosis in determining the fate of thyrocytes in autoimmune thyroid disease has been the topic of intense investigation. It is now clear that thyrocytes from patients with Hashimoto's thyroiditis are destroyed as a result of an apoptotic process. However, there is no general consensus on whether the intrathyroidal lymphocytes or the thyrocytes themselves are responsible for their death. The use of a wide range of techniques has contributed to the assessment of this process both in situ on thyroid sections and in vitro on thyroid cell preparations. The apoptosis field of research is rapidly evolving and as the pathways to cell death become unravelled, novel methods will emerge. As each technique offers some advantage, it is critical to know the most suitable method for a specific study. Equally, each method also has intrinsic limitations. Thus, to achieve reliable results, it is necessary to use more than one technique per study. In addition, techniques related to the measurement of the expression of pro-apoptotic and anti-apoptotic genes have been contributing to the study of the susceptibility of the cells to apoptosis and/or to their ability to kill themselves or neighbouring cells. In this review we will focus on the most relevant techniques.
Mirakian
R
R
Department of Immunology, St. Bartholomew's and The Royal London School of Medicine and Dentistry, UK. R.M.Mirakian@mds.qmv.ac.uk
Nye
K
K
Palazzo
F F
FF
Goode
A W
AW
Hammond
L J
LJ
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D004247:DNA / Q000032:analysis; D053222:Fas Ligand Protein; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008562:Membrane Glycoproteins / Q000032:analysis; D008854:Microscopy, Electron; D013961:Thyroid Gland / Q000473:pathology* / Q000648:ultrastructure; D013967:Thyroiditis, Autoimmune / Q000473:pathology*; D019014:fas Receptor / Q000032:analysis
D017304:Annexin A5; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D019014:fas Receptor; D004247:DNA