Protection of oxidative damage by aqueous extract from Antrodia camphorata mycelia in normal human erythrocytes.
Antrodia camphorata (A. camphorata) is well known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of aqueous extract from A. camphorata mycelia to protect normal human erythrocytes against oxidative damage in vitro. Oxidative hemolysis and lipid/protein peroxidation of erythrocytes induced by the aqueous peroxyl radical [2,2'-Azobis(2-amidinopropane) dihydrochloride, AAPH] were suppressed by A. camphorata mycelia in a time-and concentration-dependent manner. A. camphorata mycelia also prevented the depletion of cytosolic antioxidant glutathione (GSH) and ATP in erythrocytes. Moreover, cultured human endothelial cell damage induced by AAPH was suppressed by A. camphorata mycelia. Interestingly, A. camphorata mycelia exhibited significant cytotoxicity against leukemia HL-60 cells but not against cultured human endothelial cells. These results imply that A. camphorata mycelia may have protective antioxidant and anticancer properties.
Hseu
You-Cheng
YC
Department of Medical Technology, Fooyin Institute of Technology, Kaohssiung, Taiwan.
Chang
Weng-Cheng
WC
Hseu
Yi-Ting
YT
Lee
Chia-Ying
CY
Yech
Yi-Jen
YJ
Chen
Pei-Chun
PC
Chen
Jing-Yi
JY
Yang
Hsin-Ling
HL
D000255:Adenosine Triphosphate / Q000378:metabolism; D000578:Amidines / Q000494:pharmacology; D002455:Cell Division; D002457:Cell Extracts / Q000494:pharmacology; D002470:Cell Survival; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D005978:Glutathione / Q000378:metabolism; D018922:HL-60 Cells; D006461:Hemolysis; D006801:Humans; D015227:Lipid Peroxidation; D016877:Oxidants / Q000494:pharmacology; D020072:Polyporales / Q000737:chemistry* / Q000502:physiology; D016897:Respiratory Burst / Q000187:drug effects*; D014867:Water / Q000737:chemistry
D000578:Amidines; D002457:Cell Extracts; D016877:Oxidants; D014867:Water; C046728:2,2'-azobis(2-amidinopropane); D000255:Adenosine Triphosphate; D005978:Glutathione