Polyphenols in red wines prevent NADH oxidation induced by peroxynitrite.
It is known that red wines have antioxidant properties owing to the presence of polyphenols and hydroxycinnamates. It has been reported that these compounds are efficient scavengers of peroxynitrite (ONOO(-)). We assayed the ONOO(-)-scavenging activity of plant polyphenols, hydroxycinnamates, and red wines using a fluormetric assay that involves the participation of ONOO(-) (200 microM) as oxidant and NADH (50-100 microM) as a target molecule. NADH oxidation was prevented by pure polyphenols and hydroxycinnamates. With the wines, the Cabernet Sauvignon showed more potent ONOO(-)-scavenging ability than the Malbecs and the blended wines. These results correlate well with the total phenol content determined by Folin-Ciocalteau assay.
Valdez
Laura B
LB
Laboratory of Free Radical Biology, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina. lbvaldez@ffyb.uba.ar
Actis-Goretta
Lucas
L
Boveris
Alberto
A
D000975:Antioxidants / Q000494:pharmacology*; D003373:Coumaric Acids / Q000494:pharmacology; D005419:Flavonoids; D016166:Free Radical Scavengers / Q000494:pharmacology*; D009243:NAD / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D016877:Oxidants / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D030421:Peroxynitrous Acid / Q000494:pharmacology*; D010636:Phenols / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D059808:Polyphenols; D014920:Wine
D000975:Antioxidants; D003373:Coumaric Acids; D005419:Flavonoids; D016166:Free Radical Scavengers; D016877:Oxidants; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D009243:NAD; D030421:Peroxynitrous Acid