[The use of the two-hybrid cloning in yeast for functional characterization of protein kinase MAK-V].
Identification of interaction partners opens a way to direct functional characterization of proteins. Several cDNAs coding for potential partners of protein kinase MAK-V/Hunk were isolated using two-hybrid cloning in yeast. Based on the partner properties, MAK-V/Hunk was assumed to play a role in tumorigenesis and tumor progression. With the previous results of two-hybrid cloning, MAK-V/Hunk was shown to participate in vesicular transport.
Korobko
I V
IV
Institute of Gene Biology, Russian Academy of Sciences, Moscow, 117334 Russia.
Korobko
E V
EV
Chupikova
N I
NI
Vanechkin
M A
MA
Smirnova
E V
EV
Kiselev
S L
SL
Georgiev
G P
GP
D015820:Cadherins; D003001:Cloning, Molecular; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D020798:Two-Hybrid System Techniques; D033921:Vesicular Transport Proteins; D015003:Yeasts / Q000235:genetics*
D015820:Cadherins; C116934:E6-target protein 1; D020690:GTPase-Activating Proteins; D008565:Membrane Proteins; C486276:Rabep1 protein, mouse; D033921:Vesicular Transport Proteins; C502406:fat1 protein, mouse; D011494:Protein Kinases; C417729:Hunk protein, mouse; D017346:Protein Serine-Threonine Kinases
Ispol'zovanie dvugibridnogo klonirovaniia v drozhzhakh dlia funktsional'noĭ kharakteristiki proteinkinazy MAK-V.