Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov..
Thirty-three clinical, dairy and industrial isolates of aerobic endospore-forming bacteria which were unreactive in routine identification tests were characterized genotypically by using amplified rDNA restriction analysis (ARDRA), 16S rDNA sequencing and DNA-DNA reassociation, and phenotypically by using fatty acid methyl ester (FAME) analysis, SDS-PAGE of whole-cell proteins, API Biotype 100 assimilation tests and 16 other routine phenotypic tests. Three isolates were identified as strains of Bacillus badius, 12 as Brevibacillus agri, including 3 strains associated with an outbreak of waterborne illness, 4 as Brevibacillus centrosporus and 2 as Brevibacillus parabrevis; 12 strains contaminating an antibiotic production plant were recognized as members of a new species, for which the name Brevibacillus invocatus is proposed, with the type strain LMG 18962T (= B2156T = CIP 106911T = NCIMB 13772T).
Logan
N A
NA
Forsyth
G
G
Lebbe
L
L
Goris
J
J
Heyndrickx
M
M
Balcaen
A
A
Verhelst
A
A
Falsen
E
E
Ljungh
A
A
Hansson
H B
HB
De Vos
P
P
D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D001424:Bacterial Infections / Q000382:microbiology; D015373:Bacterial Typing Techniques; D004275:DNA, Ribosomal / Q000032:analysis; D003611:Dairy Products / Q000382:microbiology; D006088:Gram-Negative Aerobic Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007218:Industrial Microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial; D014871:Water Microbiology
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S