Simple and rapid quantification of the non-nucleoside reverse transcriptase inhibitors nevirapine, delavirdine, and efavirenz in human blood plasma using high-performance liquid chromatography with ultraviolet absorbance detection.
A simple reversed-phase high-performance liquid chromatography assay for the simultaneous quantitative determination of three HIV non-nucleoside reverse transcriptase inhibitors (nevirapine, delavirdine, and efavirenz) in human blood plasma is described. The method was validated over the range of 10 ng/ml to 50 microg/ml for nevirapine, 25 ng/ml to 25 microg/ml for delavirdine, and 10 ng/ml to 10 microg/ml for efavirenz. The method is accurate (average accuracies over eight concentrations ranging from 87.3 to 113%), and precise (within-day and between-day precision measures ranging from 0.12 to 7.9% and 0.26 to 5.9%, respectively). All three non-nucleoside reverse transcriptase inhibitors proved to be stable under various conditions. Due to its simplicity, this assay can readily be used for investigational or clinical monitoring of plasma concentrations.
Rezk
Naser L
NL
Pharmacology Core, Center for AIDS Research, 808 Brinkhouse-Bullitt, CB #7525, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. naser2@med.unc.edu
Tidwell
Richard R
RR
Kashuba
Angela D M
AD
D000480:Alkynes; D048588:Benzoxazines; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003521:Cyclopropanes; D020008:Delavirdine / Q000097:blood*; D015658:HIV Infections / Q000097:blood; D015497:HIV-1; D006801:Humans; D019829:Nevirapine / Q000097:blood*; D010078:Oxazines / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000097:blood*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D000480:Alkynes; D048588:Benzoxazines; D003521:Cyclopropanes; D010078:Oxazines; D018894:Reverse Transcriptase Inhibitors; D019829:Nevirapine; D020008:Delavirdine; C098320:efavirenz