title: Barotolerant E.coli Induced by High Hydrostatic Pressure.
abstract: The barotolerant E.coli TG1P, DH5alphaP, HB101P were obtained from TG1,DH5alpha, HB101 after treatment with high hydrostatic pressure, and the survival rates of the formers raised 2--3 magnitude, from 1.06x10(-6), 2.98x10(-4), 3.65x10(-3) to 8.31x10(-3), 3.40x10(-1), 1.69x10(-1),respectively. Comparison of partial proteomes of wild and barotolerant bacteria of TG1 and DH5alpha with two-dimensional electrophoresis showed that three proteins were obviously highly expressed in barotolerant bacteria. The N terminal sequence and molecular weight of one protein was shown to be V(L)EAGEFFMRA and 21 kD, with high identity to a known outmembrane protein in E.coli.
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China. kcruan@sunm.shcnc.ac.cn
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Jiong
authors.initials: J
authors.lastname: Li
authors.forename: Kang-Cheng
authors.initials: KC
authors.lastname: Ruan