Antigenic characterization of morphologically distinct Anaplasma marginale isolates using a panel of monoclonal antibodies.
The present study, describes the antigenic characterization of a Brazilian isolate of Anaplasma marginale with appendage (tail). A panel of monoclonal antibodies (McAbs) was produced and tested by the indirect fluorescent antibody test (IFAT), ELISA and Western blotting, and used to characterize two isolates of A. marginale (one with appendage and another without appendage). Among the clones produced, eight recognized antigenic proteins, with molecular weights varying from 18.4 to 66kDa. In Western blotting, the McAb reacted against a 45kDa antigen, which was shown, by the IFAT, to be located in the tail. Immunocytochemistry confirmed the tail specificity of the monoclonal reacting against the 45kDa antigen. The panel of McAb produced has a potential use in discriminating morphologically distinct A. marginale isolates. The present study, demonstrates the occurrence of antigenic diversity among Brazilian isolates of A. marginale.
Gonçalves Ruiz
Patrícia M
PM
Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Passos
Lygia M F
LM
Martins
Maria Sônia
MS
Patarroyo
Joaquin H
JH
Ribeiro
Múcio F B
MF
D000709:Anaplasma / Q000145:classification / Q000276:immunology* / Q000648:ultrastructure; D000712:Anaplasmosis / Q000276:immunology / Q000382:microbiology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000032:analysis*; D015153:Blotting, Western / Q000662:veterinary; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D016253:Microscopy, Immunoelectron / Q000662:veterinary; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial