Developmental expression patterns of alpha1H T-type Ca2+ channels during spermatogenesis and organogenesis in mice.
The objectives of the present study were to investigate the expression patterns of T-type Ca2+ channel mRNA during spermatogenesis and organogenesis in mice. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to identify the subtypes of calcium channels present in the round spermatids isolated from mouse testes by flow cytometry. Transcripts of L-type (alpha1D), non-L-type (alpha1E) and T-type Ca2+ channels were detected in round spermatids. Analysis of PCR products of T-type Ca2+ channels indicated that only alpha1H subunits were detected in round spermatids. The appearance and differential distribution of alpha1H T-type Ca2+ channel mRNA during mouse spermatogenesis and postimplantation embryogenesis (embryonic (E) days E9, E12, E15) were investigated by in situ hybridization with digoxigenin-labeled RNA probes coupled with alkaline phosphatase detection. In testes from adult and immature mice (postnatal 2 and 3 weeks), alpha1H T-type Ca2+ channel mRNA was expressed in all developing germ cells and sertoli cells. On E9 and E12, tissues of the central nervous system, such as the telencephalon, expressed alpha1H T-type Ca2+ channel mRNA. On E15, signals were detected throughout all organs of the embryo. These findings indicate that the expression of alpha1H T-type Ca2+ channels is spatio-temporally regulated during spermatogenesis and organogenesis.
Son
Weon-Young
WY
Department of Life Science, Sogang University, Seoul 121-742, Korea.
Han
Ching-Tack
CT
Lee
Jae-Ho
JH
Jung
Kyu-Yong
KY
Lee
Hyoung-Min
HM
Choo
Young-Kug
YK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020747:Calcium Channels, T-Type / Q000235:genetics*; D004247:DNA; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D038081:Organogenesis / Q000235:genetics*; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013087:Spermatids; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000378:metabolism
D020747:Calcium Channels, T-Type; D012333:RNA, Messenger; D004247:DNA