[Suppression of the metastatic potential of oncogene v-src-transformed cells as a result of activity of the exogenous DAP kinase].
Hamster tumor cell lines obtained with the Rous sarcoma virus and characterized by a high metastatic activity in vitro were transfected with the gene for C2+/calmodulin-dependent serine-threonine death-associated protein kinase (DAPk). Expression of DAPk in tumor cells dramatically reduced their survival in the blood of syngenic animals and their ability to produce metastases, but did not affect their tumorigenicity or the primary tumor growth. The DAPk-induced change in the metastatic phenotype was not accompanied by substantial changes in production and phosphorylation of v-Src or focal adhesion proteins (focal adhesion kinase and paxilline). The resulting system of transfected cells with a modulated metastatic potential provide a convenient model to study the molecular mechanisms of tumor progression at various steps.
Zueva
E Sh
ESh
Institute of Carcinogenesis, Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478 Russia.
Chevkina
E M
EM
Kimkhi
A
A
Tatosian
A G
AG
D000818:Animals; D051017:Apoptosis Regulatory Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D015197:Carcinogenicity Tests; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D017364:Collagenases / Q000378:metabolism; D006224:Cricetinae; D003598:Cytoskeletal Proteins / Q000378:metabolism; D064550:Death-Associated Protein Kinases; D005347:Fibroblasts / Q000473:pathology*; D051416:Focal Adhesion Protein-Tyrosine Kinases; D016391:Genes, src; D008647:Mesocricetus; D009362:Neoplasm Metastasis; D051419:Paxillin; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D014162:Transfection
D051017:Apoptosis Regulatory Proteins; D003598:Cytoskeletal Proteins; D051419:Paxillin; D010750:Phosphoproteins; D011505:Protein-Tyrosine Kinases; D051416:Focal Adhesion Protein-Tyrosine Kinases; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D017364:Collagenases
Podavlenie metastaticheskogo potentsiala kletok, transformirovannykh onkogenom v-src, kak rezul'tat aktivnosti ékzogennoĭ DAP-kinazy.