title: Remnant lipoprotein particles are taken up into myocardium through VLDL receptor--a possible mechanism for cardiac fatty acid metabolism.
abstract: The VLDL (very low-density lipoprotein) receptor is a peripheral lipoprotein receptor expressing in fatty acid active tissues abundantly. In the Balb/c fasting mice, VLDL receptor as well as LPL (lipoprotein lipase), FAT (fatty acid translocase)/CD36, H-FABP (heart-type fatty acid-binding protein), ACS (acyl-CoA synthetase) and LCAD (long-chain acyl-CoA dehydrogenase) expressions increased. An electron microscopic examination indicated the lipid droplets that accumulated in the hearts of fasting Balb/c mice. During the development of SD (Sprague-Dawley) rats, VLDL receptor, LPL, FAT/CD36, H-FABP, ACS, and LCAD mRNAs concomitantly increased with growth. However, PK (pyruvate kinase) mRNA expression was negligible. In cultured neonatal rat cardiomyocytes, VLDL receptor expression increased with days in culture. Oil red-O staining showed that cardiomyocytes after 7 days in culture (when the VLDL receptor protein is present) accumulated beta-migrating VLDL. Thereby, we showed that the cardiac VLDL receptor pathway for delivery of remnant lipoprotein particles might be part of a cardiac fatty acid metabolism.
authors.affiliation: Tohoku University Gene Research Center, Sendai, Japan
authors.forename: Akihisa
authors.initials: A
authors.lastname: Kamataki
authors.forename: Sadao
authors.initials: S
authors.lastname: Takahashi
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Masamura
authors.forename: Tadao
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hattori
authors.forename: Hironobu
authors.initials: H
authors.lastname: Naiki
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yamada
authors.forename: Jinya
authors.initials: J
authors.lastname: Suzuki
authors.forename: Isamu
authors.initials: I
authors.lastname: Miyamori
authors.forename: Juro
authors.initials: J
authors.lastname: Sakai
authors.forename: Takahiro
authors.initials: T
authors.lastname: Fujino
authors.forename: Tokuo T
authors.initials: TT
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004705:Endocytosis; D005215:Fasting; D005227:Fatty Acids / Q000378:metabolism*; D006321:Heart / Q000254:growth & development; D007700:Kinetics; D008055:Lipids / Q000032:analysis; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D019301:Oleic Acid / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D008055:Lipids; D008079:Lipoproteins, VLDL; D012333:RNA, Messenger; D011973:Receptors, LDL; C036053:VLDL receptor; D019301:Oleic Acid