title: Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA.
abstract: Fission yeast Cid13 and budding yeast Trf4/5 are members of a newly identified nucleotidyltransferase family conserved from yeast to man. Trf4/5 are thought to be essential DNA polymerases. We report that Cid13 is a poly(A) polymerase. Unlike conventional poly(A) polymerases, which act in the nucleus and indiscriminately polyadenylate all mRNA, Cid13 is a cytoplasmic enzyme that specifically targets suc22 mRNA that encodes a subunit of ribonucleotide reductase (RNR). cid13 mutants have reduced dNTP pools and are sensitive to hydroxyurea, an RNR inhibitor. We propose that Cid13 defines a cytoplasmic form of poly(A) polymerase important for DNA replication and genome maintenance.
authors.affiliation: Department of Molecular Biology, The Scripts Research Institute, La Jolla, CA 92037, USA.
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Saitoh
authors.forename: Andrei
authors.initials: A
authors.lastname: Chabes
authors.forename: W Hayes
authors.initials: WH
authors.lastname: McDonald
authors.forename: Lars
authors.initials: L
authors.lastname: Thelander
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Paul
authors.initials: P
authors.lastname: Russell
mesh_terms: D002467:Cell Nucleus / Q000201:enzymology* / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology* / Q000235:genetics; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D004261:DNA Replication / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005057:Eukaryotic Cells / Q000166:cytology / Q000201:enzymology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D005820:Genetic Testing; D015698:Genomic Library; D006918:Hydroxyurea / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation / Q000187:drug effects / Q000235:genetics; D009687:Nuclear Proteins; D011062:Polynucleotide Adenylyltransferase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012264:Ribonucleotide Reductases / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D005656:Fungal Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins; D004247:DNA; D012264:Ribonucleotide Reductases; C080927:SUC22 protein, S pombe; C459739:Cid13 protein, S pombe; D011062:Polynucleotide Adenylyltransferase; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase; D006918:Hydroxyurea