Expression of heme oxygenase-1 in the lung and liver tissues in a rat model of burns.
To investigate the functional significance of heme oxygenase (HO)-1 as having anti-oxidant activity in burns, a rat model of 30% burns was prepared, and the lungs and livers were extirpated for immunohistological examination (indirect immunoenzyme techniques). Plasma TNF-alpha levels were significantly higher in the burn group than in the control group, but there was no significant difference in total bilirubin levels between the groups. HO-1 expression was found histologically in the perivascular cells of the lung and liver. It was also found, though only at low levels, in liver parenchymal cells. These results suggest that HO-1 is expressed in the lung and liver tissues in a rat model of burns.
Nakae
Hajime
H
Department of Emergency and Critical Care Medicine, Akita University School of Medicine, Japan. nakaeh@doc.med.akita-u.ac.jp
Inaba
Hideo
H
D000818:Animals; D001663:Bilirubin / Q000097:blood; D002056:Burns / Q000097:blood / Q000201:enzymology*; D004195:Disease Models, Animal; D006419:Heme Oxygenase (Decyclizing) / Q000032:analysis*; D051547:Heme Oxygenase-1; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry*; D008168:Lung / Q000737:chemistry*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D014409:Tumor Necrosis Factor-alpha; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D001663:Bilirubin