title: Introduction of point mutations into cloned genes.
authors.affiliation: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
authors.forename: Brendan
authors.initials: B
authors.lastname: Cormack
authors.forename: Irene
authors.initials: I
authors.lastname: Castaño
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D016296:Mutagenesis; D016254:Mutagenesis, Insertional / Q000379:methods; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D017354:Point Mutation; D016012:Poisson Distribution; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*