title: Tropomyosin and gelsolin cooperate in controlling the microfilament system.
abstract: Tropomyosin has been shown to cause annealing of gelsolin-capped actin filaments. Here we show that tropomyosin is highly efficient in transforming even the smallest gelsolin-actin complexes into long actin filaments. At low concentrations of tropomyosin, the effect of tropomyosin depends on the length of the actin oligomer, and the cooperative nature of the process is a direct indication that tropomyosin induces a conformational change in the gelsolin-actin complexes, altering the structure at the actin (+) end such that capping by gelsolin is abolished. At increased concentrations of tropomyosin, heterodimers, trimers, and tetramers are converted to actin filaments. In addition, evidence is presented demonstrating that gelsolin, once removed from the (+) end of the actin, can reassociate with the newly formed tropomyosin-decorated actin filaments. Interestingly, the binding of gelsolin to the tropomyosin-actin filament complexes saturates at 2 gelsolin molecules per 14 actin and 2 tropomyosins, i.e. two gelsolins per tropomyosin-regulatory unit along the filament. These observations support the view that both tropomyosin and gelsolin are likely to have important functions in addition to those proposed earlier.
authors.affiliation: Department of Cell Biology, The Wenner-Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Nyakern-Meazza
authors.forename: Kartik
authors.initials: K
authors.lastname: Narayan
authors.forename: Clarence E
authors.initials: CE
authors.lastname: Schutt
authors.forename: Uno
authors.initials: U
authors.lastname: Lindberg
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D019281:Dimerization; D004305:Dose-Response Relationship, Drug; D018260:Gelsolin / Q000378:metabolism*; D007700:Kinetics; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D011485:Protein Binding; D011487:Protein Conformation; D013997:Time Factors; D014335:Tropomyosin / Q000378:metabolism*
chemical_list: D000199:Actins; D018260:Gelsolin; D014335:Tropomyosin