title: Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae.
abstract: In Saccharomyces cerevisiae the nicotinic acid moiety of NAD+ can be synthesized from tryptophan using the kynurenine pathway or incorporated directly using nicotinate phosphoribosyl transferase (NPT1). We have identified the genes that encode the enzymes of the kynurenine pathway and for BNA5 (YLR231c) and BNA6 (YFR047c) confirmed that they encode kynureninase and quinolinate phosphoribosyl transferase respectively. We show that deletion of genes encoding kynurenine pathway enzymes are co-lethal with the Deltanpt1, demonstrating that no other pathway for the synthesis of nicotinic acid exists in S. cerevisiae. Also, we show that under anaerobic conditions S. cerevisiae is a nicotinic acid auxotroph.
authors.affiliation: Centre de Génétique Moléculaire, Laboratoire propre du CNRS, associé à l'Université Pierre et Marie Curie, Gif-sur-Yvette, France.
authors.forename: Cristina
authors.initials: C
authors.lastname: Panozzo
authors.forename: Magdalena
authors.initials: M
authors.lastname: Nawara
authors.forename: Catherine
authors.initials: C
authors.lastname: Suski
authors.forename: Roza
authors.initials: R
authors.lastname: Kucharczyka
authors.forename: Marek
authors.initials: M
authors.lastname: Skoneczny
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Bécam
authors.forename: Joanna
authors.initials: J
authors.lastname: Rytka
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Herbert
mesh_terms: D000332:Aerobiosis / Q000502:physiology; D000693:Anaerobiosis / Q000502:physiology; D017353:Gene Deletion; D005800:Genes, Fungal; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D009525:Niacin / Q000096:biosynthesis* / Q000378:metabolism; D010430:Pentosyltransferases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D050608:Sodium-Phosphate Cotransporter Proteins; D050611:Sodium-Phosphate Cotransporter Proteins, Type III; D027981:Symporters / Q000502:physiology; D014364:Tryptophan / Q000378:metabolism; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)
chemical_list: D050608:Sodium-Phosphate Cotransporter Proteins; D050611:Sodium-Phosphate Cotransporter Proteins, Type III; D027981:Symporters; D009243:NAD; D009525:Niacin; D014364:Tryptophan; D010430:Pentosyltransferases; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating); D006867:Hydrolases; C021480:kynureninase; C021864:nicotinate phosphoribosyltransferase