CD56 is expressed exclusively on CD3+ T lymphocytes in canine peripheral blood.
CD56+ cells in canine blood leukocytes were characterized by flow-cytometric analysis of peripheral blood of 30 healthy adult beagle-dogs (15 males and 15 non-pregnant females). In 19 of the 30 dogs, anti human CD56 antibody, Leu-19, reacted with 8.8-21.7% of peripheral blood lymphocytes. All CD56+ cells simultaneously expressed CD3 molecules on their surface. Further phenotypic analysis revealed that 50.6+/-13.1% of the CD56+ cells showed CD4-CD8+ phenotype and 43.7+/-10.1% showed CD4+CD8- phenotype. Expression intensity of CD56 on the CD4-CD8+CD56+ cells was significantly higher than that on CD4+CD8-CD56+ cells (P<0.001). These findings indicate that CD56, which is a neural cell adhesion molecule, is uniquely expressed on subsets of T lymphocytes in canine peripheral blood.
Otani
Isao
I
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan.
Niwa
Tsuyoshi
T
Tajima
Masazumi
M
Ishikawa
Akira
A
Watanabe
Takehisa
T
Tsumagari
Shigehisa
S
Takeishi
Masatoshi
M
Kanayama
Kiichi
K
D000818:Animals; D019002:CD56 Antigen / Q000032:analysis*; D004285:Dogs / Q000097:blood* / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D015870:Gene Expression; D008297:Male; D016176:T-Lymphocyte Subsets / Q000737:chemistry*
D019002:CD56 Antigen; D016650:Fluorescein-5-isothiocyanate