title: Genotype of CYP3AP1 associated with CYP3A activity in Chinese Han population.
abstract: OBJECTIVE
  To investigate the distribution of genotype of CYP3AP1 in Chinese Han population and the correlation with CYP3A activity.
  
  
  METHODS
  Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed in CYP3AP1 genotype analysis; using midazolam as probe drug, CYP3A activity of 191 Chinese healthy subjects was measured by plasma 1'-hydroxymidazolam/midazolam (1'-OH-MDZ/MDZ) ratio at 1 h after oral administration of 7.5 mg midazolam.
  
  
  RESULTS
  There was significant difference of CYP3A activity in different genotypes of CYP3AP1 in vivo (P <0.05). The activity of CYP3A in homozygous A(-44) (CYP3AP1*3/CYP3AP1*3) is lower than heterozygous A(-44)G (CYP3AP1*1/CYP3AP1*3), and the CYP3A activity in homozygous G(-44) (CYP3AP1*1/CYP3AP1*1) is t he highest.
  
  
  CONCLUSIONS
  There was association between the genotype of CYP3AP1 and increased activity of CYP3A in vivo.
authors.affiliation: Pharmacogenetics Research Institute, Xiang-Ya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Chen
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Chen
authors.forename: Nan
authors.initials: N
authors.lastname: He
authors.forename: Zhao-Qian
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Chang-Hong
authors.initials: CH
authors.lastname: Jiang
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Hong-Hao
authors.initials: HH
authors.lastname: Zhou
mesh_terms: D000293:Adolescent; D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics* / Q000378:metabolism*; D044466:Asian People / Q000235:genetics; D051544:Cytochrome P-450 CYP3A; D005006:Ethnicity; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008874:Midazolam / Q000097:blood* / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D001189:Aryl Hydrocarbon Hydroxylases; C465138:Cyp3a23-3a1 protein, rat; D051544:Cytochrome P-450 CYP3A; D010089:Oxidoreductases, N-Demethylating; D008874:Midazolam