Characteristics of myofibres in the masseter muscle of mice during postnatal growth period.
Functional maturation of muscles is related to the constitutional proportion of muscle protein isoforms during development and growth. Although the mouse masseter muscle (MS) is classified as a fast limb muscle, its functions are different from those of a limb muscle. This study investigated the differentiation of myosin heavy chain (MHC) isoforms during the postnatal development periods in mouse MS and mouse tibialis anterior (TA), which is a fast limb muscle. Many anti-MHC slow-type-positive fibres were observed in neonatal MS and TA; these fibres decreased during development. Adult MS was composed of anti-MHC fast-type-positive fibres. MHC isoforms in MS were composed of MHC-2a and MHC-2d soon after birth. MHC-2b was expressed, but MHC-2a was not seen after 21 days. Expression of MHC-2b agreed with the weaning period, that is 2-3 weeks after birth. This fact suggested that the transformation from suckling to mastication changed the MHC isoforms during this period. In this study, the expressions of MHC-2b agree with the weaning period.
Gojo
K
K
Department of Anatomy and Oral Health Science Centre, Tokyo Dental College, Chiba City, Chiba, Japan. kgojo@tdc.ac.jp
Abe
S
S
Ide
Y
Y
D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D007536:Isomerism; D008297:Male; D008406:Masseter Muscle / Q000033:anatomy & histology* / Q000502:physiology; D051379:Mice / Q000033:anatomy & histology* / Q000502:physiology; D008813:Mice, Inbred ICR; D018485:Muscle Fibers, Skeletal / Q000737:chemistry*; D018995:Myosin Heavy Chains / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D018995:Myosin Heavy Chains