[Studies of F1 of transgenic allotetraploid hybrids of Carassius auratus red var. (Female) x Cyprinus carpio (Male)].
The tetraploid fish has been developed by assortative breeding the hybrids of Carassius auratus red var. (Female) x cyprinus carpio (Male), which has the stable genetic characters and can reproduce themselves. An "all-fish" recombinant DNA construct (pCAgcGHc) containing common carp beta-actin gene promoter and cDNA for grass carp (Ctenopharyngodon idella) growth hormone gene was introduced into fertilized eggs of the allotetroploid fish through microinjection as soon as artificial insemination was done. Artificial insemination was carried out between the female and the male transgenic allotetraploid fish which contain the "all-fish" recombinant DNA construct (pCAgcGHc) and are the biggest in the size. Fifty F1 samples of transgenic allotetraploid fish of 150 days and 50 allotetraploid fish (regarded as the control) were chosen, and the weight and the body length of each were measured, the results showed that F1 of transgenic allotetraploid fish of 150 days had obvious growth dominance compared with the control. Genomic DNA of tail fin was extracted from 20 F1 of transgenic allotetraploid fish of 150 days and the control. Proper primers were introduced to check whether the sample had the transgene. Pa, the upstream primer, is located in beta-actin promoter, and Pg, the downstream primer, is located in growth hormone cDNA for grass carp (gcGHc). The transgene was detected in 90% F1 of transgenic allotetraploid fish in tail fin DNA by polymerase chain reaction (PCR) amplification. Sperm could be squeezed out from a few F1 of transgenic allotetraploid fish of 150 days, however, this phenomenon did not exist in the controls. The importance of forming the pure line of transgenic allotetraploid was elucidate in the paper.
Feng
Hao
H
College of Life Science, Hunan Normal University, Changsha 410081, China. fenghao_99@yahoo.com
Zeng
Zhi-Qiang
ZQ
Liu
Shao-Jun
SJ
Zhang
Xuan-Jie
XJ
Zhou
Gong-Jian
GJ
Li
Jian-Zhong
JZ
Liu
Yun
Y
Wang
Ya-Ping
YP
Chen
Shang-Ping
SP
Hu
Wei
W
Zhu
Zuo-Yan
ZY
D000818:Animals; D030801:Animals, Genetically Modified; D001835:Body Weight / Q000235:genetics; D002347:Carps / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D006054:Goldfish / Q000235:genetics*; D006824:Hybridization, Genetic; D008297:Male; D016133:Polymerase Chain Reaction; D011123:Polyploidy
D004247:DNA