Priming of dolphin neutrophil respiratory burst by recombinant tumor necrosis factor alpha.
We studied the effects of recombinant dolphin tumor necrosis factor alpha (rdoTNFalpha) on the respiratory burst activity of dolphin neutrophils. rdoTNFalpha enhanced the luminol-dependent chemiluminescence response of dolphin neutrophils induced by concanavalin-A, opsonized zymosan, and heated plasma, but not that induced by phorbol myristate acetate. The TNF-associated priming activity was concentration- and preincubation time-dependent, and heat-instable. These data suggest that, as in human neutrophils, TNFalpha enhances the respiratory burst in dolphin neutrophils that follows short-term incubation with various receptor-mediated agonists.
Itou
Takuya
T
Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan. takuya@brs.nihon-u.ac.jp
Shoji
Youko
Y
Shiraishi
Rikiya
R
Sugisawa
Hitoshi
H
Endo
Tomoko
T
Inoue
Yuuki
Y
Sakai
Takeo
T
D000818:Animals; D004289:Dolphins; D009504:Neutrophils / Q000187:drug effects*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha