title: The Trimera mouse: a system for generating human monoclonal antibodies and modeling human diseases.
abstract: A Trimera mouse is constructed through a three-step process. Firstly, a normal mouse host is rendered immuno-incompetent by a lethal split-dose total body irradiation. Secondly, the myeloid and erythroid lineages are reconstituted by transplantation of bone marrow cells from a genetically immune-deficient mouse donor. Thirdly. the resulting preconditioned mouse is transplanted with human cells or tissues that can be maintained in the foreign, yet supporting, environment for a considerable period of time. Immunization of Trimera mice, engrafted with human immune cells, induces a strong human immune response, thereby enabling generation of human therapeutic monoclonal antibodies (mAbs) via hybridoma technology. Transplantation of infected human tissue into the preconditioned mice results in the creation of Trimera mouse models for human diseases.
authors.affiliation: XTL Biopharmaceuticals Ltd, Kiryat Weizmann Science Park, Rehovot, Israel. eilan@xtlbio.com
authors.forename: Ehud
authors.initials: E
authors.lastname: Ilan
authors.forename: Rachel
authors.initials: R
authors.lastname: Eren
authors.forename: Ido
authors.initials: I
authors.lastname: Lubin
authors.forename: Ofer
authors.initials: O
authors.lastname: Nussbaum
authors.forename: Arie
authors.initials: A
authors.lastname: Zauberman
authors.forename: Shlomo
authors.initials: S
authors.lastname: Dagan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D004195:Disease Models, Animal; D006509:Hepatitis B / Q000276:immunology / Q000473:pathology; D006515:Hepatitis B virus / Q000276:immunology; D006526:Hepatitis C / Q000276:immunology / Q000473:pathology; D051379:Mice; D008817:Mice, Mutant Strains; D009369:Neoplasms / Q000276:immunology / Q000473:pathology; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal