title: Kozakia baliensis gen. nov., sp. nov., a novel acetic acid bacterium in the alpha-proteobacteria.
abstract: Four bacterial strains were isolated from palm brown sugar and ragi collected in Bali and Yogyakarta, Indonesia, by an enrichment culture approach for acetic acid bacteria. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four isolates constituted a cluster separate from the genera Acetobacter, Gluconobacter, Acidomonas, Gluconacetobacter and Asaia with a high bootstrap value in a phylogenetic tree. The isolates had high values of DNA-DNA similarity (78-100%) between one another and low values of the similarity (7-25%) to the type strains of Acetobacter aceti, Gluconobacter oxydans, Gluconacetobacter liquefaciens and Asaia bogorensis. The DNA base composition of the isolates ranged from 56.8 to 57.2 mol% G+C with a range of 0-4 mol%. The major quinone was Q-10. The isolates oxidized acetate and lactate to carbon dioxide and water, but the activity was weak, as with strains of Asaia bogorensis. The isolates differed from Asaia bogorensis strains in phenotypic characteristics. The name Kozakia baliensis gen. nov., sp. nov., is proposed for the four isolates. Strain Yo-3T (= NRIC 0488T = JCM 11301T = IFO 16664T = DSM 14400T) was isolated from palm brown sugar collected in Bali, Indonesia, and was designated as the type strain.
authors.forename: Puspita
authors.initials: P
authors.lastname: Lisdiyanti
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Yantyati
authors.initials: Y
authors.lastname: Widyastuti
authors.forename: Susono
authors.initials: S
authors.lastname: Saono
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Seki
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Yamada
authors.forename: Tai
authors.initials: T
authors.lastname: Uchimura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Komagata
mesh_terms: D019342:Acetic Acid / Q000378:metabolism*; D016947:Acetobacteraceae / Q000145:classification* / Q000235:genetics* / Q000502:physiology; D020561:Alphaproteobacteria / Q000145:classification / Q000235:genetics / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004275:DNA, Ribosomal / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D019342:Acetic Acid