[Determination of fractional and subfractional blood lipoprotein compositions with small angle X-ray scattering (a comparison with a biochemical method)].
Highly accurate and fast method for determination of quantitative and qualitative compositions of serum lipoprotein (LP) fractions and subfractions, using small-angle X-ray scattering on the "Siemens" difractometr, has been developed. The method allows to determine the concentrations of serum cholesterol (CH), triglycerides (TG), high-density lipoprotein (HDL)-CH, low-density lipoprotein (LDL)-CH, phospholipides (FL) and total lipids. It allows to determine the levels of CH, TG, FL in LP subfractions and concentrations of 5 very-low-density lipoprotein, intermedius-density lipoprotein, 3 LDL (large, intermedius and small dense particles) and 5 HDL subfractions. The method is based on the elaborated united model of all LP fractions and subfractions as a result of generalization and analysis of literature data about LP composition and size. New method was proved to be highly reproductive. Strong positive correlation exists between serum levels of CH, TG, HDL-CH determined by enzymatic biochemical and our methods. The method is simple to perform and of high diagnostic value, and can, therefore, be applied to clinical purposes.
Tuzikov
F V
FV
Institute of Molecular Biology RSC of Virusology and Biotechnology Vektor, Kolthovo, Novosibirsk Region.
Ragino
Iu I
IuI
Tuzikova
N A
NA
Ivanova
M V
MV
Galimov
R V
RV
Nikitin
Iu P
IuP
D002784:Cholesterol / Q000097:blood / Q000737:chemistry; D004798:Enzymes; D006801:Humans; D008074:Lipoproteins / Q000097:blood / Q000737:chemistry*; D008075:Lipoproteins, HDL / Q000097:blood / Q000737:chemistry; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry; D011933:Reagent Kits, Diagnostic; D012542:Scattering, Radiation; D014280:Triglycerides / Q000097:blood / Q000737:chemistry; D014965:X-Rays
D004798:Enzymes; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D011933:Reagent Kits, Diagnostic; D014280:Triglycerides; D002784:Cholesterol
Opredelenie fraktsionnogo i subfraktsionnogo sostavov lipoproteinov krovi metodom malouglovogo rentgenovskogo rasseianiia (sravnenie s biokhimicheskim metodom).