Evaluation of the portable Cepheid SmartCycler real-time PCR machine for the rapid diagnosis of foot-and-mouth disease.
The ability of the portable Cepheid SmartCycler real-time PCR machine to detect foot-and-mouth disease (FMD) virus sensitively and accurately was evaluated by comparing the results of the analyses of nasal swab and serum samples from experimentally infected animals with those obtained from the real-time PCR assay currently in use in the laboratory. The results indicated that the ability of the machine to detect viral RNA is greatly affected by the PCR reagents used for the assay. When it was used with PCR beads it was unable to detect weakly positive samples, but when TaqMan core reagents were used for the assay, its sensitivity was significantly increased. The machine could be used for the laboratory-based detection of FMD; however, as with all assays, significant optimisation of assay conditions as well as solid validation of the technique is required.
Hearps
A
A
Institute for Animal Health, Pirbright, Woking, Surrey.
Zhang
Z
Z
Alexandersen
S
S
D000818:Animals; D004279:DNA, Viral / Q000097:blood*; D004867:Equipment Design; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000473:pathology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000302:isolation & purification; D009666:Nose / Q000821:virology; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000592:standards / Q000662:veterinary*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012756:Sheep
D004279:DNA, Viral