title: Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov.
abstract: The characteristics of the fusiform species Bacteroides forsythus, isolated from human periodontal pockets, were examined. 165 rDNA sequence analysis confirmed that B. forsythus was not a species within the genus Bacteroides sensu stricto. Although B. forsythus was phylogenetically related to Bacteroides distasonis and Bacteroides merdae in the phylogenetic tree, the ratio of anteiso-15:0 to iso-15:0 in whole-cell methanolysates of B. forsythus was different from those of B. distasonis, B. merdae and other Bacteroides species. B. forsythus did not grow on medium containing 20% bile, but members of the Bacteroides fragilis group did. B. forsythus was the only species tested that was trypsin-positive in API ZYM tests. The dehydrogenase enzyme pattern was of no use for the differentiation of B. forsythus and the B. fragilis group. On the basis of these data, a new genus, Tannerella, is proposed for Bacteroides forsythus, with one species, Tannerella forsythensis corrig., gen. nov., comb. nov. The type strain of Tannerella forsythensis is JCM 10827T (= ATCC 43037T).
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Masahito
authors.initials: M
authors.lastname: Suzuki
authors.forename: Makoto
authors.initials: M
authors.lastname: Umeda
authors.forename: Isao
authors.initials: I
authors.lastname: Ishikawa
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Benno
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001439:Bacteroides / Q000737:chemistry / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D001646:Bile / Q000378:metabolism; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010514:Periodontal Pocket / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D011933:Reagent Kits, Diagnostic; D024482:Vitamin K 2