[The effect of inactivation of co-stimulated particles B7.1 (CD80), B7.2 (CD86) on T lymphocyte activity].
We have studied the effect of CD80 (B7.1) and CD86 (B7.2) costimulatory molecules inactivation on IL-4 and IFN-gamma production by T lymphocytes. T cells were received from nonatopic asthmatic and healthy subjects. We have added anti-CD80 or anti-CD86 monoclonal antibodies to the monocyte/lymphocyte (1:5) cultures. In nonatopic asthma patients compare to control group inactivation of CD80 significantly reduced IFN-gamma production by T lymphocytes (2172/2368 pg/ml; p < 0.02). Blockade of CD86 decreased IFN-gamma production non-significantly (2858/3317 pg/ml; p < 0.3). In both studied groups anti-CD80 antibodies did not diminish T lymphocyte production of IL-4 (control: 630/725 pg/ml; nonatopic asthma: 718/742 pg/ml). Anti-CD86 antibodies reduced the IL-4 concentration in culture supernatants (630/523 pg/ml; 718/659 pg/ml), but the changes were nonsignificant.
Rutkowski
Ryszard
R
Klinika Alergologii i Chorób Wewnetrznych Akademii Medycznej w Białymstoku.
Moniuszko
Tadeusz
T
Stasiak-Barmuta
Anna
A
Rutkowska-Rogacz
Danuta
D
Siergiejko
Zenon
Z
D000328:Adult; D015703:Antigens, CD / Q000276:immunology*; D001249:Asthma / Q000276:immunology*; D018122:B7-1 Antigen / Q000276:immunology*; D051940:B7-2 Antigen; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis*; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D013601:T-Lymphocytes / Q000378:metabolism*
D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D008562:Membrane Glycoproteins; D015847:Interleukin-4; D007371:Interferon-gamma
Wpływ inaktywacji czastek kostymulujacych B7.1 (CD80), B7.2 (CD86) na aktywność limfocytów T.