Ketamine and the inhibition of albumin extravasation in chemical peritonitis in rat.
OBJECTIVE
It was previously reported that topical ketamine inhibits albumin extravasation in a rat chemical peritonitis model. Using the same model, the present study investigated whether intravenous ketamine inhibited this extravasation.


METHODS
Twenty-four rats anaesthetized with pentobarbital (75 mg kg(-1)) were randomly assigned to two groups: ketamine and a 0.9% NaCl (saline) group (n = 12 each). Ketamine 1% or saline 0.1 mL kg(-1) min(-1) was given intravenously for 60 min to the respective group. After the abdomen had been opened, peritonitis was elicited by topically applying a filter paper containing 0.02 M HCl 0.07 mL onto the surface of the appendix or caecum for 5 min. Fifteen minutes after removal of the filter paper, Evans' blue dye (50 mg kg(-1)) was injected intravenously. The extravasated dye was colorimetrically quantified by a spectrophotometer at 620 nm.


RESULTS
The infusion of ketamine significantly reduced Evans' blue extravasation: 5.26 (range 4.18-6.34) microg per 100 mg tissue compared with the saline group control: 6.81 (5.93-7.69) microg per 100 mg tissue (P < 0.05).


CONCLUSIONS
It is suggested that ketamine anaesthesia may reduce albumin extravasation in inflammatory tissues.
Hirota
K
K
University of Hirosaki School of Medicine, Department of Anesthesiology, Japan. masuika@cc.hirosaki-u.ac.jp
Ishihara
H
H
Matsuki
A
A
D000418:Albumins / Q000378:metabolism*; D000778:Anesthetics, Dissociative / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002199:Capillary Permeability / Q000187:drug effects; D003124:Colorimetry; D004396:Coloring Agents; D005070:Evans Blue; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D006851:Hydrochloric Acid; D007649:Ketamine / Q000494:pharmacology*; D008297:Male; D010538:Peritonitis / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000418:Albumins; D000778:Anesthetics, Dissociative; D004396:Coloring Agents; D018691:Excitatory Amino Acid Antagonists; D005070:Evans Blue; D007649:Ketamine; D006851:Hydrochloric Acid