Specific induction of apoptosis by 1,8-cineole in two human leukemia cell lines, but not a in human stomach cancer cell line.
We have investigated the effects of 1,8-cineole [the main component of essential oil prepared from bay-leaves Laurus nobilis L.)] on DNA of human leukemia cell lines, Molt 4B, HL-60 and stomach cancer KATO III cells. Specific induction of apoptosis by 1,8-cineole was observed in human leukemia Molt 4B and HL-60 cells, but not in human stomach cancer KATO III cells. Morphological changes showing apoptotic bodies were observed in the human leukemia HL-60 cells treated with 1,8-cineole. The fragmentations of DNA by cineole to oligonucleosomal-sized fragments that is a characteristic of apoptosis were concentration- and time-dependent in Molt 4B and HL-60 cells, but not in KATO III cells. The present study shows that the suppression growth by 1,8-cineole in the leukemia cell lines results from the induction of apoptosis by this compound.
Moteki
Hiroyuki
H
Faculty of Bioresources, Mie University, Tsu-city, Mie 514-0001, Japan.
Hibasami
Hiroshige
H
Yamada
Yayoi
Y
Katsuzaki
Hirotaka
H
Imai
Kunio
K
Komiya
Takashi
T
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D003511:Cyclohexanols / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000187:drug effects*; D000077591:Eucalyptol; D006801:Humans; D027442:Laurus / Q000737:chemistry; D007938:Leukemia / Q000473:pathology*; D039821:Monoterpenes; D013274:Stomach Neoplasms / Q000473:pathology*; D013729:Terpenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D000972:Antineoplastic Agents, Phytogenic; D003511:Cyclohexanols; D004273:DNA, Neoplasm; D039821:Monoterpenes; D013729:Terpenes; D000077591:Eucalyptol