title: Determination of fenoterol in human plasma by HPLC with fluorescence detection after derivatization.
abstract: A new method for the determination of fenoterol is described, which uses HPLC separation with fluorescence detection. Dobutamine is employed as an internal standard. The separation was achieved on a short reversed phase column with a mobile phase consisting of water, acetonitrile and methanol. Prior to chromatography both analytes are derivatized with 9-chloroformyl-carbazole. Isolation of the analytes from plasma is carried out by liquid-liquid extraction into 2-butanol after protein precipitation with acetonitrile. The method is capable of estimating fenoterol concentrations in the sub-nanogram per ml range with sufficient accuracy and precision. The determination of fenoterol can now be carried out in the average laboratory without radiolabelled material.
authors.affiliation: Department of Clinical Pharmacology, University of Göttingen, Robert-Koch-Strasse 40, D-37075 Gottingen, Germany. imeineke@med.uni-goettingen.de
authors.forename: Ingolf
authors.initials: I
authors.lastname: Meineke
authors.forename: Hannelore
authors.initials: H
authors.lastname: Steinmetz
authors.forename: Skaidrit
authors.initials: S
authors.lastname: Kramer
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Gleiter
mesh_terms: D000318:Adrenergic beta-Agonists / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005280:Fenoterol / Q000097:blood*; D006801:Humans; D011247:Pregnancy; D015203:Reproducibility of Results
chemical_list: D000318:Adrenergic beta-Agonists; D005280:Fenoterol