[Permeabilization of tumor cells induced by pulsed electric fields in vitro].
The permeabilization of tumor cells in vitro under the action of pulsed electric fields with a duration of 6 mks in the range of amplitudes 1-7 kV/cm was studied. In the mode of excitation in the ambience of localized plasma discharge in a chamber of special design, an enhanced damage to cells in suspension was observed. It is assumed that the enhancement is due to the synchronous action of the electric field and acoustic shock wave pulses. In the mode without the plasma breakdown of ambience, when the pulse duration of electric field of intensity of 1-2 kV/cm was increased to 60 mks, the efficiency of permeabilization increases nearly by one order. The experimental results are compared with the known theoretical models of cell membrane electroporation.
Andriianov
Iu V
IuV
Troitsk Institute of Innovation and Thermonuclear, State Research Center Russian Federation, Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia.
Andriianova
O N
ON
Golovanov
M V
MV
Dobrynin
Ia V
IaV
Kozodoĭ
P V
PV
Smirnov
V P
VP
D000162:Acoustics; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D016923:Cell Death; D002463:Cell Membrane Permeability; D004560:Electricity; D018274:Electroporation; D051379:Mice; D014407:Tumor Cells, Cultured / Q000473:pathology* / Q000648:ultrastructure
Permeabilizatsiia opukholevykh kletok in vitro pod deĭstviem impul'snykh élektricheskikh poleĭ.