Gene transfer into eukaryotic cells using activated polyamidoamine dendrimers.
The development of efficient methods to transfer genes into eukaryotic cells is important for molecular biotechnology. A number of different technologies to mediate gene transfer have been developed over the last 35 years, but most have drawbacks such as cytotoxicity, low efficiency and/or restricted applicability. Activated polyamidoamine (PAMAM)-dendrimers provide a new technology for gene transfer that offers significant advantages over classical methods. Reagents based on this technology provide high gene transfer efficiencies, minimal cytotoxicity, and can be used with a broad range of cell types. This technology could also be useful for in vivo gene transfer in gene therapy applications.
Dennig
Jörg
J
QIAGEN GmbH, Hilden, Germany. j.dennig@de.qiagen.com
Duncan
Emma
E
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials; D001709:Biotechnology / Q000379:methods; D004247:DNA / Q000235:genetics*; D005057:Eukaryotic Cells; D015316:Genetic Therapy / Q000379:methods; D006001:Glycoconjugates / Q000138:chemical synthesis / Q000737:chemistry; D051379:Mice; D011073:Polyamines; D014162:Transfection / Q000379:methods*
D001672:Biocompatible Materials; D006001:Glycoconjugates; D011073:Polyamines; D004247:DNA