Evidence of a tetradocosahexaenoic cardiolipin in some marine bivalves.
Separation of phospholipid classes in lipid extracts from the scallop Pecten maximus, the Pacific oyster Crassostrea gigas, and the blue mussel Mytilus edulis was conducted using HPLC. An isolated polar lipid fraction was found to contain a very high level of DHA, up to 80 mol% of the total FA. MS with electrospray ionization in the positive-ion mode, tandem MS (MS-MS) and multidimensional NMR spectroscopy were used to analyze the detailed chemical structure of this polar lipid fraction. The isolated fraction contained exclusively cardiolipin (CL) molecules, predominantly in a form with four docosahexaenoyl chains (Do4CL). To the best of our knowledge, this is the first time that such a CL form has been analytically characterized and described in these three bivalve species. This tetradocosahexaenoic CL is presumed to reflect a specific adaptation in bivalves that enhances the structural and functional mechanisms of biomembranes in response to variations in environmental conditions (temperature, salinity, emersion).
Kraffe
Edouard
E
Unité Mixte CNRS 6521, Université de Bretagne Occidentale, Brest, France.
Soudant
Philippe
P
Marty
Yanic
Y
Kervarec
Nelly
N
Jehan
Philippe
P
D000818:Animals; D002308:Cardiolipins / Q000032:analysis* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004281:Docosahexaenoic Acids / Q000032:analysis* / Q000302:isolation & purification*; D005227:Fatty Acids / Q000032:analysis; D008974:Mollusca / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular
D002308:Cardiolipins; D005227:Fatty Acids; D004281:Docosahexaenoic Acids