title: Purification and partial gene sequence of the tyrosine decarboxylase of Lactobacillus brevis IOEB 9809.
abstract: Some lactic acid bacteria contain a tyrosine decarboxylase (TDC) which converts tyrosine to tyramine, a biogenic amine frequently encountered in fermented food and wine. Purification and microsequencing of the TDC of Lactobacillus brevis IOEB 9809 allowed us to determine a partial sequence of the TDC gene encoding 264 amino acids of the enzyme. Analysis of this protein sequence revealed typical features of pyridoxal phosphate-dependent amino acid decarboxylases while not any known decarboxylase was closely related to the TDC of L. brevis IOEB 9809. In addition, we could detect other L. brevis strains carrying a TDC gene in a rapid assay based on the polymerase chain reaction.
authors.affiliation: Laboratoire de Biotechnologie et Microbiologie Appliquée, Faculté d'OEnologie, Unité Associée INRA-Université Victor Ségalen Bordeaux 2, 351, cours de la Libération, 33405, Talence cedex, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Lucas
authors.forename: Aline
authors.initials: A
authors.lastname: Lonvaud-Funel
mesh_terms: D000595:Amino Acid Sequence; D005798:Genes, Bacterial / Q000235:genetics; D007778:Lactobacillus / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014445:Tyrosine Decarboxylase / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D014445:Tyrosine Decarboxylase