title: CD56 is expressed exclusively on CD3+ T lymphocytes in canine peripheral blood.
abstract: CD56+ cells in canine blood leukocytes were characterized by flow-cytometric analysis of peripheral blood of 30 healthy adult beagle-dogs (15 males and 15 non-pregnant females). In 19 of the 30 dogs, anti human CD56 antibody, Leu-19, reacted with 8.8-21.7% of peripheral blood lymphocytes. All CD56+ cells simultaneously expressed CD3 molecules on their surface. Further phenotypic analysis revealed that 50.6+/-13.1% of the CD56+ cells showed CD4-CD8+ phenotype and 43.7+/-10.1% showed CD4+CD8- phenotype. Expression intensity of CD56 on the CD4-CD8+CD56+ cells was significantly higher than that on CD4+CD8-CD56+ cells (P<0.001). These findings indicate that CD56, which is a neural cell adhesion molecule, is uniquely expressed on subsets of T lymphocytes in canine peripheral blood.
authors.affiliation: Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Otani
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Niwa
authors.forename: Masazumi
authors.initials: M
authors.lastname: Tajima
authors.forename: Akira
authors.initials: A
authors.lastname: Ishikawa
authors.forename: Takehisa
authors.initials: T
authors.lastname: Watanabe
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Tsumagari
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Takeishi
authors.forename: Kiichi
authors.initials: K
authors.lastname: Kanayama
mesh_terms: D000818:Animals; D019002:CD56 Antigen / Q000032:analysis*; D004285:Dogs / Q000097:blood* / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D015870:Gene Expression; D008297:Male; D016176:T-Lymphocyte Subsets / Q000737:chemistry*
chemical_list: D019002:CD56 Antigen; D016650:Fluorescein-5-isothiocyanate