An evolutionarily conserved mediator of plant disease resistance gene function is required for normal Arabidopsis development.
Plants recognize many pathogens through the action of a diverse family of proteins called disease resistance (R) genes. The Arabidopsis R gene RPM1 encodes resistance to specific Pseudomonas syringae strains. We describe an RPM1-interacting protein that is an ortholog of TIP49a, previously shown to interact with the TATA binding protein (TBP) complex and to modulate c-myc- and beta-catenin-mediated signaling in animals. Reduction of Arabidopsis TIP49a (AtTIP49a) mRNA levels results in measurable increases of two R-dependent responses without constitutively activating defense responses, suggesting that AtTIP49a can act as a negative regulator of at least some R functions. Further, AtTIP49a is essential for both sporophyte and female gametophyte viability. Thus, regulators of R function overlap with essential modulators of plant development.
Holt
Ben F
BF
Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA.
Boyes
Douglas C
DC
Ellerström
Mats
M
Siefers
Nicholas
N
Wiig
Aaron
A
Kauffman
Scott
S
Grant
Murray R
MR
Dangl
Jeffery L
JL
D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002999:Clone Cells; D017124:Conserved Sequence; D004265:DNA Helicases; D019143:Evolution, Molecular; D017353:Gene Deletion; D005786:Gene Expression Regulation; D017343:Genes, Plant; D007113:Immunity, Innate / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000378:metabolism; D011549:Pseudomonas / Q000472:pathogenicity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D013489:Suppression, Genetic
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; C095014:RPM1 protein, Arabidopsis; D011993:Recombinant Fusion Proteins; D004265:DNA Helicases; C459939:TIP49a protein, Arabidopsis