Effects of Cyclosporine A and Highly Expressed Bcl-2 on Apoptosis of HL-60 Cells Induced by EGTA.
Effects of mitochondrial permeability transition pore-specific inhibitor cyclosporine A (CsA) and highly expressed Bcl-2 on the apoptosis of HL-60 cells induced by EGTA were studied. Detection of apoptotic peak by flow cytometry, fluorescent microscope observation of chromatin condensation with double staining of PI and Hoechst33342 and DNA ladder analysis all demonstrated that CsA obviously enhanced the apoptosis of HL-60 cells induced by EGTA, while highly expressed Bcl-2 completely blocked it. It is revealed by mitochondrial membrane potential (deltapsi(m)) fluorescent probes rhodamine 123 and CMXRos that the deltapsi(m) decreased in the apoptosis of HL-60 cells induced by EGTA. CsA enhanced the decrease of deltapsi(m), but highly expressed Bcl-2 increased deltapsi(m) of HL-60 cells about 2-fold and completely blocked the decrease of deltapsi(m).
Ma
Jing
J
Department of Biochemistry and Center for Biopharmaceutical Research, College of Life Science, Zhongshan University, Guangzhou 510275, China. ls36@zsu.edu.cn
Fu
Nai-Yang
NY
Xu
An-Long
AL