Gene regulation by human orthologs of Drosophila heterochromatin protein 1.
Heterochromatin protein 1 (HP1) functions in gene silencing, transcriptional regulation, and chromatin remodeling. Drosophila HP1 enhances position effect variegation and has also been shown to repress transcription of some euchromatic genes and activate some heterochromatic ones. The purpose of this study was to determine if human orthologs of Drosophila HP1 differentially regulate transcription by identifying genes that are targets for regulation by HP1 family proteins in human cells. Using a gene expression array, we identify several genes regulated by overexpression of the three human HP1 family proteins HP1(Hsalpha), HP1(Hsbeta), and HP1(Hsgamma). We show further that overexpressed HP1(Hsalpha) and HP1(Hsbeta) repress the transcription of four human genes while overexpressed HP1(Hsgamma) enhances transcription of the same genes. These results show that different human HP1 family proteins can potentially repress or activate the same genes.
Hwang
Kyu-Kye
KK
Department of Medicine, College of Physicians & Surgeons, Columbia University, 630 West 168th Street, 10th Floor, Room 508, New York, NY 10032, USA.
Worman
Howard J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004330:Drosophila; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014162:Transfection; D019076:Transgenes
D002868:Chromosomal Proteins, Non-Histone; D018076:DNA, Complementary; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D000090266:Chromobox Protein Homolog 5; D049452:Green Fluorescent Proteins; D012313:RNA