Analysis of gene expression profile in p130(Cas)-deficient fibroblasts.
p130(Cas) (Cas) is a docking protein that becomes tyrosine phosphorylated in v-Src- or v-Crk-transformed cells and in integrin-stimulated cells. Cas -/- fibroblasts show defects in stress fiber formation, cell spreading, cell migration, and transformation by activated Src. To further characterize the role of Cas in signaling, we compared the expression profile in Cas -/- fibroblasts with that in Cas-re-expressing fibroblasts using the microarray methods. In Cas -/- fibroblasts, the expression of heme oxygenase 1 and caveolin-1 was reduced, but the expression of procollagen 1 alpha 1, procollagen 3 alpha 1, procollagen 11 alpha 1, elastin, periostin, TSC-36, and MARCKS was enhanced. The domains in Cas necessary for the change varied among these genes. Activated Src reduced the expression of most of these genes both in Cas -/- and in Cas +/+ fibroblasts. These results suggest the existence of signaling pathways that emanate from Cas to gene expression.
Nakamoto
Tetsuya
T
Department of Hematology and Oncology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Suzuki
Takahiro
T
Huang
Jinhong
J
Matsumura
Tomoko
T
Seo
Sachiko
S
Honda
Hiroaki
H
Sakai
Ryuichi
R
Hirai
Hisamaru
H
D000818:Animals; D002478:Cells, Cultured; D050719:Crk-Associated Substrate Protein; D005347:Fibroblasts / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D051379:Mice; D010750:Phosphoproteins / Q000172:deficiency* / Q000235:genetics*; D011506:Proteins; D050717:Retinoblastoma-Like Protein p130; D015398:Signal Transduction / Q000235:genetics
C493842:Bcar1 protein, mouse; D050719:Crk-Associated Substrate Protein; D010750:Phosphoproteins; D011506:Proteins; D050717:Retinoblastoma-Like Protein p130