title: Effect of enrofloxacin treatment on plasma endotoxin during bovine Escherichia coli mastitis.
abstract: OBJECTIVE
  To investigate the effect of enrofloxacin on endotoxin resorption during bovine Escherichia coli mastitis.
  
  
  METHODS
  12 healthy early post partum Holstein cows.
  
  
  METHODS
  Mastitis was induced by intramammary infusion of 10(4) cfu E. coli P4:032. Six cows were treated twice according to the usual enrofloxacin therapy: 5 mg/kg enrofloxacin 1) intravenously at 10 h and 2) subcutaneously at 30 h after challenge. The other 6 cows served as non-treated controls.
  
  
  METHODS
  Blood and milk samples were collected at several time points after challenge. LPS in plasma was quantified using the limulus amoebocyte lysate (LAL) assay. The somatic cell count (SCC) and cfu of milk samples were also analysed.
  
  
  RESULTS
  Occasional LPS peaks were detected in the plasma of 2 control cows at 6 h post-challenge and of 1 enrofloxacin-treated cow at 10 h post-challenge (P < 0.01 and P < 0.05, respectively, in comparison with time 0), just before enrofloxacin treatment. After enrofloxacin treatment, no significant LPS amounts were detected in the plasma of treated cows, but neither in the control cows.
  
  
  CONCLUSIONS
  During induced coliform mastitis, LPS resorption in plasma occured only sporadically and within 10 h post-challenge. Whereas enrofloxacin treatment clearly limited bacterial growth in milk, significant effects on LPS resorption could not be detected. This suggests that enrofloxacin treatment of E. coli mastitis is predominantly beneficial by its bactericidal activity and is not associated with enhanced resorption of endotoxins.
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Physiology, Biochemistry and Biometrics, Merelbeke, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Dosogne
authors.forename: E
authors.initials: E
authors.lastname: Meyer
authors.forename: A
authors.initials: A
authors.lastname: Sturk
authors.forename: J
authors.initials: J
authors.lastname: van Loon
authors.forename: A M
authors.initials: AM
authors.lastname: Massart-Leën
authors.forename: C
authors.initials: C
authors.lastname: Burvenich
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use*; D002417:Cattle; D002452:Cell Count; D004731:Endotoxins / Q000032:analysis / Q000097:blood*; D000077422:Enrofloxacin; D004927:Escherichia coli Infections / Q000097:blood / Q000662:veterinary*; D005260:Female; D024841:Fluoroquinolones; D008414:Mastitis, Bovine / Q000378:metabolism* / Q000503:physiopathology; D008892:Milk / Q000737:chemistry / Q000166:cytology; D015363:Quinolones / Q000627:therapeutic use*
chemical_list: D000890:Anti-Infective Agents; D004731:Endotoxins; D024841:Fluoroquinolones; D015363:Quinolones; D000077422:Enrofloxacin