Differences in MHC class I genes between strains of rainbow trout (Oncorhynchus mykiss).
In rainbow trout there is only one dominant classical MHC class I locus, Onmy-UBA, for which four very different allelic lineages have been described. The purpose of the present study was to determine if Onmy-UBA polymorphism could be used for strain characterisation. This was performed by lineage-specific PCR investigation of 30 fish, each of the Nikko and Donaldson strains, and by sequence analysis of 25 of the amplified DNA fragments. Two new MHC class I lineages were detected in addition to the four previously described lineages, thus six distinct lineages were observed within the fish examined (Sal-MHCIa*A-F). The distribution of lineages appeared to be strain-specific. For example, the lineage Sal-MHCIa*A was very common in the Nikko strain but could not be detected in the Donaldson strain. Analysis of MHC class I variation may help to elucidate relationships between strains and the roles of MHC alleles in disease resistance.
Xia
Chun
C
Inland Station/National Research Institute of Aquaculture, Tamaki, Mie, Japan.
Kiryu
Ikunari
I
Dijkstra
Johannes Martinus
JM
Azuma
Teruo
T
Nakanishi
Teruyuki
T
Ototake
Mitsuru
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005784:Gene Amplification; D005805:Genes, MHC Class I / Q000235:genetics*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid