title: Melatonin reduces memory changes and neural oxidative damage in mice treated with D-galactose.
abstract: To investigate the role of melatonin in D-galactose-induced amnesic mice, the avoidance/escape and water maze tests were performed to evaluate their learning and memory function. Spectrophotometry was employed to determine the content of thiobarbituric acid-reactive substances (TBARS) and the activities of antioxidative enzymes in the brain. The present results demonstrate that D-galactose-induced amnesic mice had significantly decreased learning and memory function. The reduced activities of superoxide dismutase and glutathione peroxidase and increased levels of TBARS were found in brain tissue of the amnesic mice. Melatonin, administered (ig) at doses of 0.1, 1, or 10 mg/kg to the D-galactose-treated mice for 3 months, was sufficient to block these changes. These data suggest that D-galactose is involved in accelerating the brain aging process by elevating free radical generation and reducing antioxidative enzyme activities in vivo. Furthermore, the antioxidative activity of melatonin on the D-galactose-treated mice may account for, at least partially, the improvement of learning and memory function in the aging and amnesic model.
authors.affiliation: Institute of Clinical Pharmacology, Anhui Medical University, Hefei, China. shenyuxi@mail.hf.ah.cn
authors.forename: Yu-Xian
authors.initials: YX
authors.lastname: Shen
authors.forename: Shu-Yun
authors.initials: SY
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Xiu-Xia
authors.initials: XX
authors.lastname: Sun
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Liu
authors.forename: Chen
authors.initials: C
authors.lastname: Dong
mesh_terms: D000647:Amnesia / Q000188:drug therapy; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005690:Galactose / Q000009:adverse effects*; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D007858:Learning / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008550:Melatonin / Q000494:pharmacology*; D008568:Memory / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D008550:Melatonin; D005690:Galactose