Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae the nicotinic acid moiety of NAD+ can be synthesized from tryptophan using the kynurenine pathway or incorporated directly using nicotinate phosphoribosyl transferase (NPT1). We have identified the genes that encode the enzymes of the kynurenine pathway and for BNA5 (YLR231c) and BNA6 (YFR047c) confirmed that they encode kynureninase and quinolinate phosphoribosyl transferase respectively. We show that deletion of genes encoding kynurenine pathway enzymes are co-lethal with the Deltanpt1, demonstrating that no other pathway for the synthesis of nicotinic acid exists in S. cerevisiae. Also, we show that under anaerobic conditions S. cerevisiae is a nicotinic acid auxotroph.
Panozzo
Cristina
C
Centre de Génétique Moléculaire, Laboratoire propre du CNRS, associé à l'Université Pierre et Marie Curie, Gif-sur-Yvette, France.
Nawara
Magdalena
M
Suski
Catherine
C
Kucharczyka
Roza
R
Skoneczny
Marek
M
Bécam
Anne Marie
AM
Rytka
Joanna
J
Herbert
Christopher J
CJ
D000332:Aerobiosis / Q000502:physiology; D000693:Anaerobiosis / Q000502:physiology; D017353:Gene Deletion; D005800:Genes, Fungal; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D009525:Niacin / Q000096:biosynthesis* / Q000378:metabolism; D010430:Pentosyltransferases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D050608:Sodium-Phosphate Cotransporter Proteins; D050611:Sodium-Phosphate Cotransporter Proteins, Type III; D027981:Symporters / Q000502:physiology; D014364:Tryptophan / Q000378:metabolism; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)
D050608:Sodium-Phosphate Cotransporter Proteins; D050611:Sodium-Phosphate Cotransporter Proteins, Type III; D027981:Symporters; D009243:NAD; D009525:Niacin; D014364:Tryptophan; D010430:Pentosyltransferases; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating); D006867:Hydrolases; C021480:kynureninase; C021864:nicotinate phosphoribosyltransferase