title: Thrombopoietin administered during induction chemotherapy to patients with acute myeloid leukemia induces transient morphologic changes that may resemble chronic myeloproliferative disorders.
abstract: Thrombopoietin (TPO), a potent stimulator of megakaryocyte and platelet production, has been used in clinical trials to reduce thrombocytopenia after chemotherapy in patients with acute myeloid leukemia (AML). We report that TPO therapy is associated with peripheral blood and bone marrow findings that can mimic myeloproliferative disorders. Peripheral blood and bone marrow samples of 13 patients with AML who received TPO were examined. A subset of bone marrow samples exhibited hypercellularity, megakaryocytic hyperplasia, and reticulin fibrosis after TPO administration. Cases demonstrated as many as 58.4 megakaryocytes per high-powerfield (MHPF) compared with 3.7 MHPF in the control group. Megakaryocytic atypia, increased mitoses, emperipolesis, intrasinusoidal megakaryocytes, and thickened trabeculae also were seen. Peripheral blood findings included leukoerythroblastosis, leukocytosis, thrombocytosis, and circulating megakaryocyte nuclei. Changes resolved within 3 months after discontinuation of TPO. This rapid resolution of the morphologic abnormalities induced by TPO distinguishes these findings from those seen in true chronic myeloproliferative disorders.
authors.affiliation: Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville 32610-0275, USA.
authors.forename: Vonda K
authors.initials: VK
authors.lastname: Douglas
authors.forename: Martin S
authors.initials: MS
authors.lastname: Tallman
authors.forename: Larry D
authors.initials: LD
authors.lastname: Cripe
authors.forename: LoAnn C
authors.initials: LC
authors.lastname: Peterson
mesh_terms: D000208:Acute Disease; D000368:Aged; D000369:Aged, 80 and over; D001853:Bone Marrow / Q000187:drug effects* / Q000473:pathology; D004359:Drug Therapy, Combination; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000627:therapeutic use; D006801:Humans; D007951:Leukemia, Myeloid / Q000150:complications* / Q000188:drug therapy / Q000473:pathology; D008533:Megakaryocytes / Q000187:drug effects / Q000473:pathology; D008875:Middle Aged; D009196:Myeloproliferative Disorders / Q000139:chemically induced* / Q000473:pathology; D020360:Neoadjuvant Therapy / Q000009:adverse effects*; D012189:Retrospective Studies; D013926:Thrombopoietin / Q000009:adverse effects*
chemical_list: D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013926:Thrombopoietin