title: Eight-hydroxy-2'-deoxyguanosine in granulosa cells is correlated with the quality of oocytes and embryos in an in vitro fertilization-embryo transfer program.
abstract: OBJECTIVE
  To determine the effects of oxidative stress on the quality of oocytes and embryos, 8-hydroxy-2'-deoxyguanosine (8-OHdG) in granulosa cells was quantitatively studied during an in vitro fertilization and embryo transfer (IVF-ET) program.
  
  
  METHODS
  Immunocytochemical staining of 8-OHdG in granulosa cells was quantitatively estimated using a charge-coupled device camera and analyzed using the National Institute of Health Image (NIH Image) freeware on a computer .
  
  
  METHODS
  Obstetrics and gynecology department in a university hospital.
  
  
  METHODS
  Ninety-six infertile couples undergoing IVF-ET treatment and intracytoplasmic sperm injection (IVF, n = 72; intracytoplasmic sperm injection, n = 24).
  
  
  METHODS
  Oocytes, granulosa cells, and follicular fluids were collected 35-36 hours after the administration of hCG.
  
  
  METHODS
  8-OHdG indices were obtained for mural [8-OHdG index (m)] and cumulus [8-OHdG index (c)] granulosa cells.
  
  
  RESULTS
  A negative correlation between the fertilization rate and both 8-OHdG indices (c and m) was found. The rate of production of good embryos also showed a negative correlation with the 8-OHdG index (m) and the 8-OHdG index (c). Negative correlations between the 8-OHdG index (c) and E2 levels in follicular fluid were observed. Endometriosis patients showed a higher 8-OHdG index (c) than did patients with other infertility causes, such as tubal, male factor, and unknown.
  
  
  CONCLUSIONS
  Oxidative stress in granulosa cells lowered fertilization rates and subsequently led to a decrease in the quality of embryos. The quality of oocytes for endometriosis patients was impaired by the presence of 8-OHdG. This might be one causative factor in infertility in endometriosis patients.
authors.affiliation: Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata, Japan.
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Seino
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Saito
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kaneko
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kawachiya
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Kurachi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism*; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology*; D004715:Endometriosis / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism; D007248:Infertility, Male / Q000378:metabolism; D008297:Male; D009865:Oocytes / Q000502:physiology*; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D004958:Estradiol; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine