title: Ubiquitin genes in rainbow trout (Oncorhynchus mykiss).
abstract: Ubiquitin is a small protein involved in intracellular proteolysis. It is highly conserved throughout eukaryotic phyla and has been detected in such diverse species as yeast, barley, Drosophila and man. A previous study showed that chromatin of rainbow trout testis contains free ubiquitin with a sequence similar to that of other phyla. In the present study, which focused on rainbow trout but included eleven other species, it is shown that fish ubiquitin genetic organisation and expression are similar to those of other phylogenetic groups through the following set of observations: (a) Multiple loci were detected, (b) These loci encode repeats of ubiquitin, (c) Although the DNA sequences are not conserved, the encoded amino acid sequences are fully conserved, (d) The expression of ubiquitin was influenced by cell culture conditions and viral infection.
authors.affiliation: Department of Aquatic Biosciences, Tokyo University of Fisheries, Japan.
authors.forename: Kazue
authors.initials: K
authors.lastname: Okubo
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yamano
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Qin
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Aoyagi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Ototake
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Hideo
authors.initials: H
authors.lastname: Fukuda
authors.forename: Johannes M
authors.initials: JM
authors.lastname: Dijkstra
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry; D015870:Gene Expression; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012313:RNA / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017421:Sequence Analysis; D017385:Sequence Homology; D013045:Species Specificity; D025801:Ubiquitin / Q000737:chemistry / Q000235:genetics*
chemical_list: D025801:Ubiquitin; D012313:RNA; D004247:DNA