title: Molecular Cloning of a Gene Suppressed by Heat Shock and Its Expression Characteristics.
abstract: On the base of a cDNA fragment cloned from a human osteosarcoma cell line (HOS-8603) which was taken as the model of heat shock by DDRT-PCR, the cDNA library was screened by the probe made from this fragment, and the colony containing full length cDNA of this cDNA fragment (HSSG-1) was identified. Sequencing data indicated that this cDNA consisted of 1 456 bp, coding for 276 amino acids. Computer aided homology analysis did not find the same sequence published. The result of RNA dot hybridization suggested that HSSG-1 was expressing in many kinds of organs and cells, and the down regulating of its expression level caused by heat shock was general. Therefore, this may be a gene that is suppressed by heat shock.
authors.affiliation: Department of Molecular Genetics, Second Military Medical University, Shanghai 200433, China. daijx@sina.com
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Dai
authors.forename: Yi-Lun
authors.initials: YL
authors.lastname: Wang
authors.forename: Feng-Juan
authors.initials: FJ
authors.lastname: Zhou
authors.forename: De-Ru
authors.initials: DR
authors.lastname: Lu
authors.forename: Yu-Jian
authors.initials: YJ
authors.lastname: Liu
authors.forename: Ren-Bao
authors.initials: RB
authors.lastname: Xu