title: Caenorhabditis elegans reticulon interacts with RME-1 during embryogenesis.
abstract: Reticulon (RTN) family proteins are localized in the endoplasmic reticulum (ER). At least four different RTN genes have been identified in mammals, but in most cases, the functions of the encoded proteins except mammalian RTN4-A and RTN4-B are unknown. Each RTN gene produces 1-3 proteins by different promoters and alternative splicing. In Caenorhabditis elegans, there is a single gene (rtn gene) encoding three reticulon proteins, nRTN-A, B, and C. mRNA of nRTN-C is expressed in germ cells and embryos. However, nRTN-C protein is only expressed during embryogenesis and rapidly disappears after hatch. By yeast two-hybrid screening, two clones encoding the same C-terminal region of RME-1, a protein functioning in the endocytic recycling, were isolated. These findings suggest that nRTN-C functions in the endocytic pathway during embryogenesis.
authors.affiliation: Department of Virology, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Iwahashi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kawasaki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kohara
authors.forename: Keiko
authors.initials: K
authors.lastname: Gengyo-Ando
authors.forename: Shohei
authors.initials: S
authors.lastname: Mitani
authors.forename: Yasumi
authors.initials: Y
authors.lastname: Ohshima
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamada
authors.forename: Koyu
authors.initials: K
authors.lastname: Hara
authors.forename: Takahito
authors.initials: T
authors.lastname: Kashiwagi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Toyoda
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002460:Cell Line; D006066:Gonads / Q000196:embryology / Q000378:metabolism; D007700:Kinetics; D010750:Phosphoproteins / Q000378:metabolism*; D018510:RNA, Helminth / Q000096:biosynthesis; D020798:Two-Hybrid System Techniques
chemical_list: D029742:Caenorhabditis elegans Proteins; D002135:Calcium-Binding Proteins; D010750:Phosphoproteins; C486205:RME-1 protein, C elegans; D018510:RNA, Helminth