Elucidation of primary (alpha(3)N) and vestigial (alpha(5)) heavy metal-binding sites in Staphylococcus aureus pI258 CadC: evolutionary implications for metal ion selectivity of ArsR/SmtB metal sensor proteins.
Despite a common evolutionary origin, individual members of the ArsR/SmtB family of bacterial metal-responsive transcriptional repressors sense a wide range of heavy-metal ions. The molecular basis for this metal ion selectivity is unclear. Here, we establish that Staphylococcus aureus plasmid pI258 CadC, a Cd(II)/Pb(II)/Bi(III)/Zn(II) sensor, contains two distinct metal-binding sites: a thiolate-rich alpha(3)N site comprised exclusively of cysteine ligands that preferentially binds larger, softer metal ions such as Cd(II), Pb(II) and Bi(III); and a second C-terminal alpha(5) site, found at the dimer interface, that is devoid of cysteine ligands and preferentially binds smaller, harder metal ions [Co(II) and Zn(II)] concurrently with metal binding to the alpha(3)N site. Optical absorption and X-ray spectroscopies reveal that the alpha(3)N site can adopt distinct coordination geometries in order to accommodate different metal ions, i.e. Cd(II), Bi(III), Co(II) and Zn(II) form distorted tetrahedral S(4) complexes, while Pb(II) adopts a trigonal S(3) complex. Characterization of mutant CadCs reveals that the alpha(3)N site is composed of Cys58 and Cys60 from the alpha(3) helix of the helix-turn-helix DNA-binding domain and Cys7 and/or Cys11 from the N-terminal "arm" of CadC; Cys11 is excluded from the Pb(II) coordination sphere. Only the thiolate-rich alpha(3)N site is metalloregulatory for repressor binding to a fluorescein-labeled cad O/P oligonucleotide upon coordination to Cd(II), Pb(II), Bi(III), Zn(II), and weakly for Co(II). Substitution of Cys60 and Cys7 with non-ligating residues specifically abrogates metal-dependent negative regulation of cad O/P binding, despite the fact that C60G and C7G CadCs maintain high affinity for metals in altered coordination complexes. These findings reveal that formation of metal coordination bonds to Cys7 and Cys60 play primary roles in transducing the allosteric response in CadC. The evolutionary implications for metal ion selectivity of ArsR/SmtB metal sensor proteins are discussed.
Busenlehner
Laura S
LS
Department of Biochemistry and Biophysics, Center for Advanced Biomolecular Research, Texas A&M University, College Station, TX 77843-2128, USA
Weng
Tsu-Chien
TC
Penner-Hahn
James E
JE
Giedroc
David P
DP
D000494:Allosteric Regulation; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D002104:Cadmium / Q000378:metabolism; D003035:Cobalt / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D019143:Evolution, Molecular; D005454:Fluorescence Polarization; D007477:Ions / Q000378:metabolism; D007854:Lead / Q000378:metabolism; D008024:Ligands; D008087:Listeria; D009682:Magnetic Resonance Spectroscopy; D008670:Metals / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000235:genetics; D013379:Substrate Specificity; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism*
D001426:Bacterial Proteins; C080050:CadC protein, Bacteria; D004268:DNA-Binding Proteins; D007477:Ions; D008024:Ligands; D008670:Metals; D012097:Repressor Proteins; D015534:Trans-Activators; D002104:Cadmium; D007854:Lead; D003035:Cobalt; D004247:DNA