Protein G ELISA for detection of antibodies against Toxoplasma SAG1 in dromedaries.
ELISA was used for detection of antibodies against the immunodominant surface antigen 1 (SAG1, synonymous P30) of Toxoplasma gondii, and peroxidase-conjugated protein G was used instead of commercially unavailable enzyme-conjugated anti-dromedary antibody. A latex agglutination test was employed to select 20 seronegative control animals, and peroxidase-conjugated protein A was used for comparison with protein G. The overall seroprevalence rate was 31.4%; males had to some extent higher seropositive rate than females (P = 0.077). Seropositive camels sampled in winter had significantly higher-antibody levels than those sampled in summer (P < 0.01). ELISA values using protein G and protein A conjugates were significantly correlated (Spearman's rho = 0.797; n = 185; P < 0.001). The results were discussed.
Abu-Zeid
Yousif A
YA
Department of Biology, Faculty of Science, United Arab Emirates University, Al Ain. abuzeid@emirates.net.ae
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan; D002162:Camelus / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D008297:Male; D015800:Protozoan Proteins / Q000276:immunology*; D012621:Seasons; D016036:Seroepidemiologic Studies; D012737:Sex Factors; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D014479:United Arab Emirates / Q000453:epidemiology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma