Changes in the leucocyte subpopulations of the palatine tonsillar crypt epithelium of pigs in response to Streptococcus suis type 2 infection.
The tonsils are portal of entry and a site of multiplication and persistence for a variety of pathogens, including Streptococcus suis (S. suis), which is a common cause of meningitis, septicemia and arthritis in pigs. Understanding the early changes that occur in the first barrier of the tonsil, i.e. the crypt epithelium, in response to S. suis infection is critical in clarifying the pathogenesis of this disease and for the future development of efficient methods of mucosal vaccination. In this study, we investigated the early changes, from 18 to 72 h, that occur in leucocyte subpopulations of the crypt epithelium of the palatine tonsils of 3-week-old pigs in response to S. suis type 2 infection. Monoclonal antibodies against leucocyte markers CD3, CD4, CD8, gammadelta T cell receptor, lambda-immunoglobulin light-chain, myeloid cells, and major histocompatibility complex class II molecule (MHC-II) were used in an avidin-biotin immunoperoxidase technique. An increase in the number of lambda-immunoglobulin light-chain positive cells (B cell subset) was noticed in crypts of S. suis-infected animals from 18 h after infection onwards. This increase was significant at 18 and 48 h after infection. The number of CD4 and CD8 cells was greater from 18 h onwards, with a significant increase at 24 and 72 h post-infection. No significant difference in numbers of CD3, gammadelta T cell receptor and MHC-II positive cells was detected in the crypts of infected animals compared to controls. Macrophages, neutrophils and crypt epithelial cells stained positively with the myeloid marker, and the area of crypt epithelium positive for this marker was increased in the crypts of infected animals, with a significant difference detected at 24 and 72 h after infection. These results suggest that there is participation of the innate immunity in the early phase of S. suis infection, represented by neutrophils, macrophages and likely epithelial cells, and that there is a potential for the initiation of both humoral and cellular responses against S. suis within the crypt epithelium of the palatine tonsil.
Salles
Monica W S
MW
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada S7N 5B4. monica.salles@usask.ca
Perez-Casal
J
J
Willson
P
P
Middleton
D M
DM
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D004848:Epithelium / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D007146:Immunoglobulin lambda-Chains; D007150:Immunohistochemistry / Q000662:veterinary; D007962:Leukocytes / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016131:Lymphocyte Subsets / Q000276:immunology* / Q000382:microbiology; D008285:Major Histocompatibility Complex / Q000276:immunology; D014066:Palatine Tonsil / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D013290:Streptococcal Infections / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D017011:Streptococcus suis / Q000254:growth & development*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000382:microbiology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D007146:Immunoglobulin lambda-Chains; D016692:Receptors, Antigen, T-Cell, gamma-delta