Molecular cloning of a vaccine antigen against infection with the larval stage of Echinococcus multilocularis.
Alveolar and cystic hydatidosis are caused by infection with the larval stages of Echinococcus multilocularis and Echinococcus granulosus, respectively. A host-protective antigen has been identified in E. granulosus. Here we identify the presence of a closely related protein in E. multilocularis, characterize and express a cDNA encoding the antigen (designated EM95), determine the structure of the em95 gene, and demonstrate that the EM95 recombinant protein can be used to induce significant levels of protection against challenge infection with E. multilocularis eggs in mice.
Gauci
Charles
C
Molecular Parasitology Laboratory, Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia. charlesg@unimelb.edu.au
Merli
Michael
M
Muller
Volker
V
Chow
Conan
C
Yagi
Kinpei
K
Mackenstedt
Ute
U
Lightowlers
Marshall W
MW
D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D003411:Arvicolinae; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004195:Disease Models, Animal; D004443:Echinococcosis / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D004445:Echinococcosis, Pulmonary / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D004446:Echinococcus / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D005260:Female; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology; D007814:Larva; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D020442:Poloxamer; D011136:Polysorbates; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D016205:Sigmodontinae; D013185:Squalene / Q000031:analogs & derivatives*; D014612:Vaccines; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology
D000276:Adjuvants, Immunologic; D000947:Antigens, Helminth; D018076:DNA, Complementary; C446034:EG95 protein, Echinococcus granulosus; C460870:EM95 protein, Echinococcus multilocularis; D015801:Helminth Proteins; D011136:Polysorbates; D011993:Recombinant Fusion Proteins; D014612:Vaccines; D014614:Vaccines, Synthetic; D020442:Poloxamer; D013185:Squalene; C019556:squalane