[Comparison of the homologous SfeI and LlaBI restriction-modification systems].
A fragment containing the SfeI restriction-modification system (RMS) operon was cloned from a Streptococcus faecalis SE72 plasmid. Nucleotide sequence analysis revealed its high (99.2%) homology to the operon for Lactococcus lactis subsp. cremoris W56 LlaBI RMS recognizing the same site, 5'-CTRYAG-3'. A substantial difference was that SfeI RMS operon had an additional 198-bp fragment and a larger gene for the putative control protein. No homology was observed between operon-flanking sequences of the two closely related species, suggesting horizontal transfer of the operon.
Okhapkina
S S
SS
Institute of Molecular Biology, Vektor State Research Center of Virology and Biotechnology, Kol'tsovo, Novosibirsk Region, 633159 Russia. sso@lae.ma.nsc.ru
Netesova
N A
NA
Golikova
L N
LN
Seregina
E V
EV
Sosnovtsev
S V
SV
Abdurashitov
M A
MA
Degtiarev
S Kh
SKh
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D021901:DNA, Intergenic; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics* / Q000378:metabolism; D013293:Enterococcus faecalis / Q000235:genetics; D022761:Gene Transfer, Horizontal; D013294:Lactococcus lactis / Q000235:genetics; D008969:Molecular Sequence Data; D009876:Operon; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D021901:DNA, Intergenic; C085497:endodeoxyribonuclease SfcI; C057737:endodeoxyribonuclease SfeI; D015252:Deoxyribonucleases, Type II Site-Specific
Sravnitel'nyĭ analiz gomologichnykh sistem restriktsii-modifikatsii SfeI i LlaBI.