Cytokinesis-defective mutants of Arabidopsis.
We have identified mutations in six previously uncharacterized genes of Arabidopsis, named club, bublina, massue, rod, bloated, and bims, that are required for cytokinesis. The mutants are seedling lethal, have morphological abnormalities, and are characterized by cell wall stubs, gapped walls, and multinucleate cells. In these and other respects, the new mutants are phenotypically similar to knolle, keule, hinkel, and pleiade mutants. The mutants display a gradient of stomatal phenotypes, correlating roughly with the severity of their cytokinesis defect. Similarly, the extent to which the different mutant lines were capable of growing in tissue culture correlated well with the severity of the cytokinesis defect. Phenotypic analysis of the novel and previously characterized loci indicated that the secondary consequences of a primary defect in cytokinesis include anomalies in body organization, organ number, and cellular differentiation, as well as organ fusions and perturbations of the nuclear cycle. Two of the 10 loci are required for both cytokinesis and root hair morphogenesis. The results have implications for the identification of novel cytokinesis genes and highlight the mechanistic similarity between cytokinesis and root hair morphogenesis, two processes that result in a rapid deposition of new cell walls via polarized secretion.
Söllner
Rosi
R
Genetics and Microbiology Institute, Ludwig Maximillian University, Maria Ward Strasse 1a, 80638 Munich, Germany.
Glässer
Gerti
G
Wanner
Gehard
G
Somerville
Chris R
CR
Jürgens
Gerd
G
Assaad
Farhah F
FF
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D018797:Cell Cycle Proteins; D002455:Cell Division / Q000235:genetics*; D002473:Cell Wall / Q000235:genetics; D046508:Culture Techniques; D008565:Membrane Proteins / Q000235:genetics; D008869:Microtubule-Associated Proteins / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D010940:Plant Proteins; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development; D050765:Qa-SNARE Proteins; D012639:Seeds / Q000235:genetics / Q000254:growth & development
C426230:AT1G12360 protein, Arabidopsis; D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C452431:HINKEL protein, Arabidopsis; C097724:KNOLLE protein, Arabidopsis; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D010940:Plant Proteins; D050765:Qa-SNARE Proteins