Kinetics of urinary recovery of five sugars after orogastric administration in healthy dogs.
OBJECTIVE
To describe the kinetics of urinary recovery (UR) of 5 sugars used for gastrointestinal permeability and mucosal function testing following orogastric administration of lactose, rhamnose, xylose, methylglucose, and sucrose.


METHODS
7 healthy male Beagles.


METHODS
A sugar solution containing lactulose, rhamnose, xylose, methylglucose, and sucrose was administered by orogastric intubation to healthy dogs. Urine samples were collected immediately before sugar solution administration (baseline) and at 2-hour intervals thereafter. The UR of the 5 sugars was determined from urine concentrations measured by high pressure liquid chromatography and pulsed amperometric detection. Percent urinary recovery (%UR) of the total UR up to 12 hours after sugar solution administration was calculated for each sugar at 2-hour intervals.


RESULTS
Mean %UR exceeded 85% for all 5 sugars at 6 hours after orogastric administration of the sugar solution and exceeded 90% after 8 hours.


CONCLUSIONS
In healthy dogs, a urine collection period of 6 hours is sufficient for gastrointestinal permeability and mucosal function testing following orogastric administration of lactulose, rhamnose, xylose, methylglucose, and sucrose.
Steiner
Jörg M
JM
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station 77843-4474, USA.
Williams
David A
DA
Moeller
Erik M
EM
D000284:Administration, Oral; D000818:Animals; D002241:Carbohydrates / Q000008:administration & dosage / Q000652:urine*; D004285:Dogs / Q000502:physiology* / Q000652:urine; D007700:Kinetics; D008297:Male
D002241:Carbohydrates