Expression of matrix metalloprotease-2 (MMP-2) and the activator membrane type 1 (MT1-MMP) in canine mammary carcinomas.
An immunohistochemical approach was used to examine the expression of MMP-2 and the activator MT1-MMP in a series of 50 canine mammary carcinomas of different histotype and stage. MMP-2 and MT1-MMP were mainly expressed in the cytoplasm of carcinoma cells and in fibroblasts. Immunolabelling for both MMP-2 and MT1-MMP was also seen on the tumour cell membranes. This labelling pattern showed no significant association with either the histological sub-type or stage of the carcinomas. Therefore, although distinct patterns of expression of MMP-2 and MT-MMP1 were shown by these carcinomas, functional studies by means of zymography would be required to provide useful information on tumour behaviour.
Papparella
S
S
Dipartimento di Patologia e Sanità Animale, Settore Anatomia Patologica, Facoltà di Medicina Veterinaria, Università degli Studi di Napoli Federico II, Via F. Delpino 1, Napoli, 80137, Italy.
Restucci
B
B
Paciello
O
O
Maiolino
P
P
D000818:Animals; D001940:Breast / Q000033:anatomy & histology / Q000201:enzymology; D002277:Carcinoma / Q000201:enzymology / Q000556:secondary / Q000662:veterinary*; D004283:Dog Diseases / Q000201:enzymology* / Q000473:pathology; D004285:Dogs; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D015674:Mammary Neoplasms, Animal / Q000201:enzymology* / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000096:biosynthesis*
D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2