Typing Y chromosome STR haplotypes using redesigned primers.
A panel of Y-specific STR loci, including DYS19, DYS389, DYS390, DYS391, DYS392, and DYS393 was analyzed using horizontal nondenaturing polyacrylamide gel electrophoresis with a discontinuous buffer system (horizontal disk-PAGE). In order to obtain correct results for the larger DYS389 and DYS392 alleles, it was necessary to design new primers that bind closer to the repeat region and lead to a significant reduction of the amplified fragment size. Using the modified primer sets the horizontal disk-PAGE results were consistent with a nondenaturing approach using fluorescent primers and a 377 automated sequencer. The modified procedure also amplifies the second repeat stretch at the duplicated DYS389 locus as a single fragment, which results in an immediate allele identification. The results indicate that horizontal disk-PAGE with silverstaining is a simple approach to type the recommended Y-specific STR markers.
Hou
Yiping
Y
Institute of Forensic Medicine, Sichuan University, Chengdu, PR China. rechtsme@wcums.edu.cn
Zhang
Ji
J
Sun
Dahong
D
Li
Yinbi
Y
Wu
Jin
J
Zhang
Sizhong
S
Prinz
Mechthild
M
D017931:DNA Primers / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005554:Forensic Medicine / Q000379:methods; D006239:Haplotypes; D006801:Humans; D008297:Male; D020080:Tandem Repeat Sequences / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*
D017931:DNA Primers