Successful diagnosis using scab for PCR specimen in Tsutsugamushi disease.
We treated a case of Tsutsugamushi disease diagnosed by polymerase chain reaction (PCR) using a scab specimen at the bite site of trombiculid mites. Otherwise the diagnosis could not be confirmed by serum antibody test nor the PCR test of blood. The genome of Rickettsia tsutsugamushi was detected and identified as the Kawasaki serotype strain. An attempt to extract the genome from the scab has not been reported, thus our data suggest that the scab is a useful specimen to confirm the diagnosis of Tsutsugamushi disease.
Ono
Akihiro
A
Department of Internal Medicine, Numata National Hospital, Gunma.
Nakamura
Kumiko
K
Higuchi
Seiichi
S
Miwa
Yoshihiro
Y
Nakamura
Kazumi
K
Tsunoda
Takeshi
T
Kuwabara
Hidemasa
H
Furuya
Yumiko
Y
Dobashi
Kunio
K
Mori
Masatomo
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001733:Bites and Stings / Q000469:parasitology*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D016680:Genome, Bacterial; D006801:Humans; D008875:Middle Aged; D008911:Minocycline / Q000627:therapeutic use; D012285:Orientia tsutsugamushi / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012612:Scrub Typhus / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D014324:Trombiculidae / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D008911:Minocycline