Effect of low- and high-virulence Yersinia enterocolitica strains on the inflammatory response of human umbilical vein endothelial cells.
Pathogenic strains of Yersinia spp. inject a set of Yop effector proteins into eukaryotic cells by using a plasmid-encoded type III secretion system. In this study, we analyzed the inflammatory response of human umbilical vein endothelial cells (HUVECs) after infection with different Yersinia enterocolitica strains. We found that both expression of intercellular adhesion molecule 1 and release of the cytokines interleukin-6 (IL-6) and IL-8 by HUVECs are downregulated in a YopP-dependent way, demonstrating that YopP plays a major role in the inflammatory response of these cells. Infection of HUVECs with several low-virulence (biotype 2, 3, and 4) and high-virulence (biotype 1B) Y. enterocolitica strains showed that biotype 1B isolates are more efficient in inhibiting the inflammatory response than low-virulence Y. enterocolitica strains and that this effect depends on the time of contact. We extended the results of Ruckdeschel et al. and found that on the basis of the presence or absence of arginine-143 of YopP (K. Ruckdeschel, K. Richter, O. Mannel, and J. Heesemann, Infect. Immun. 69:7652-7662, 2001) all the Y. enterocolitica strains used fell into two groups, which correlate with the low- and high-virulence phenotypes. In addition, we found that high-virulence strains inject more YopP into the cytosol of eukaryotic target cells than do low-virulence strains.
Denecker
Geertrui
G
Christian de Duve Institute of Cellular and Molecular Pathology and Faculté de Médecine, Université Catholique de Louvain, Brussels, Belgium.
Tötemeyer
Sabine
S
Mota
Luís J
LJ
Troisfontaines
Paul
P
Lambermont
Isabelle
I
Youta
Carine
C
Stainier
Isabelle
I
Ackermann
Martin
M
Cornelis
Guy R
GR
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D003593:Cytoplasm; D015536:Down-Regulation; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D006367:HeLa Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology; D014774:Virulence; D015008:Yersinia enterocolitica / Q000276:immunology* / Q000472:pathogenicity
D001426:Bacterial Proteins; D015850:Interleukin-6; D016209:Interleukin-8; C109227:YopP protein, Yersinia; D018799:Intercellular Adhesion Molecule-1