title: Correlation between HSP90 induction kinetics in murine leukemia cells and the amount of cisplatin over a wide range of cytostatic concentrations.
abstract: The induction of HSP90 in murine erythroleukemia cells, clone F4 N, by cisplatin (DDP) was examined using indirect immunofluorescence and avidin-biotin technique, and compared with cisplatin cytotoxicity. A reverse dependence of HSP90 induction time was found on a wide range of cisplatin concentrations (0.5-10 microM), which proved to be cytostatic up to 48 h of continuous treatment. Thus, the observed induction pattern of HSP90 in F4 N cells strictly correlated with their high tolerance toward DDP. This indicates that HSP90 might be responsible, at least in part, for cisplatin resistance of F4 N cells.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia. roumi@obzor.bio21.bas.bg
authors.forename: Roumiana L
authors.initials: RL
authors.lastname: Detcheva
authors.forename: Andrey N
authors.initials: AN
authors.lastname: Kenderov
authors.forename: Angelina I
authors.initials: AI
authors.lastname: Russinova
authors.forename: Nadejda C
authors.initials: NC
authors.lastname: Spassovska
authors.forename: Kolyo G
authors.initials: KG
authors.lastname: Kolev
authors.forename: Konstantin C
authors.initials: KC
authors.lastname: Grancharov
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002945:Cisplatin / Q000494:pharmacology; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis* / Q000187:drug effects; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D051379:Mice; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D018841:HSP90 Heat-Shock Proteins; D002945:Cisplatin