The presence of D-beta-aspartic acid-containing peptides in elastic fibers of sun-damaged skin: a potent marker for ultraviolet-induced skin aging.
Biologically uncommon d-aspartyl residues have been reported in proteins of various elderly tissues. We prepared a polyclonal antibody against d-beta-Asp-containing peptide and examined its immunoreactivity in the skin. The antibody recognized integrated or disintegrated elastic fibers in the sun-exposed skin but not in the sun-protected skin of the elderly donors. Western blot analysis of the proteins isolated from sun-damaged skin demonstrated that the 50 kDa protein was immunoreactive with both antibodies for d-beta-Asp-containing peptide and elastin. Ultraviolet (UV) irradiation on normal skin caused the appearance of d-beta-Asp-containing peptide-immunoreactive fibers in the dermis. These results suggest that UV irradiation is closely related to the formation of d-beta-Asp in the elastic fibers of skin. We propose that the antibody could be a useful indicator for sun damage of the skin.
Fujii
Noriko
N
Research Reactor Institute, Kyoto University Kumatori, Sennan, Osaka 590-0494, Japan. nfujii@hl.rri.kyoto-u.ac.jp
Tajima
Shingo
S
Tanaka
Nobuhiko
N
Fujimoto
Norihiro
N
Takata
Takumi
T
Shimo-Oka
Tadashi
T
D000368:Aged; D000375:Aging / Q000528:radiation effects*; D001224:Aspartic Acid / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D002648:Child; D026603:D-Aspartic Acid / Q000032:analysis / Q000276:immunology; D004547:Elastic Tissue / Q000737:chemistry*; D005145:Face / Q000033:anatomy & histology / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D026581:Isoaspartic Acid / Q000032:analysis / Q000276:immunology; D008875:Middle Aged; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D012867:Skin / Q000033:anatomy & histology / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D015415:Biomarkers; D026581:Isoaspartic Acid; D010455:Peptides; D011506:Proteins; D001224:Aspartic Acid; D026603:D-Aspartic Acid