Fluorescence assay for studying the ability of macrolides to induce production of ribosomal methylase.
A screening assay to test the inducing capacity of macrolides by fusing the attenuator of the inducible erm(B) gene from Streptococcus pneumoniae HM28 with the gfpmut1 gene has been designed. Fluorescence was detected under UV light around disks impregnated with inducer macrolides (erythromycin or azithromycin) but not with noninducer ketolides. Induction could be quantified by fluorometry.
Clarebout
Gervais
G
Service de Microbiologie, UPRES EA 2128, Hôpital Côte de Nacre, Université de Caen, 14033 Caen Cedex, France.
Leclercq
Roland
R
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D005453:Fluorescence; D018942:Macrolides; D008780:Methyltransferases / Q000096:biosynthesis*; D008826:Microbial Sensitivity Tests; D013296:Streptococcus pneumoniae
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase