Apolipoprotein C3 SstI polymorphism and triglyceride levels in Asian Indians.
BACKGROUND
A close association between Sst I polymorphism in the 3' untranslated region of the apolipoproteinC3 (APOC3) gene and levels of plasma triglycerides (TG) had been reported by different investigators. Hypertriglyceridemia(HTG) is a known risk factor for coronary artery disease (CAD) in the context of Asian Indians. We conducted a study on the relationship between APOC3 SstI polymorphism (S1S1, S1S2 and S2S2 genotypes) and plasma TG levels in a group of 139 male healthy volunteers from Northern India.


METHODS
DNA samples were analyzed by polymerase chain reaction (PCR) followed by SstI digestion. Digested PCR products were run on 3% agarose gel and visualized by ethidium bromide staining.


RESULTS
Rare S2 allele was highly prevalent in our study population (0.313) as compared to the Caucasians (0.00-0.11). The genotypic distribution was in agreement with Hardy-Weinberg equilibrium. S2 allele was almost two times more prevalent in the HTG group (N = 34) as compared to NTG group (N = 105) (p = 0.001). Multiple logistic regression revealed S1S2 individuals had age-adjusted odds ratio of 2.43 (95%CI = 0.99-6.01, p = 0.054) and S2S2 had 9.9 (95%CI = 2.66-37.29, p = 0.0006) for developing HTG in comparison to S1S1 genotype.


CONCLUSIONS
Our study shows a significant association between rare S2 allele and HTG in Asian Indians.
Chhabra
S
S
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi-110029, India. shivani_chhabra@hotmail.com
Narang
R
R
Krishnan
L R
LR
Vasisht
S
S
Agarwal
D P
DP
Srivastava
L M
LM
Manchanda
S C
SC
Das
N
N
D053305:Apolipoprotein C-III; D001056:Apolipoproteins C / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D005838:Genotype; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood / Q000453:epidemiology / Q000235:genetics; D007194:India / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D014280:Triglycerides / Q000097:blood*; D044465:White People / Q000235:genetics
D053305:Apolipoprotein C-III; D001056:Apolipoproteins C; D014280:Triglycerides; C024408:endodeoxyribonuclease SacI; D015252:Deoxyribonucleases, Type II Site-Specific