Molecular characterization of Cryptosporidium sp. isolated from northern Alaskan caribou (Rangifer tarandus).
Cryptosporidium sp. was found in 3 out of 49 caribou (Rangifer tarandus) from northern Alaska. Segments of both the 18S ribosomal RNA and the heat shock protein genes were amplified from the caribou isolate and compared with that obtained from an isolate from a wild white-tailed deer (Odocoileus virginianus) in Virginia as well as other species and isolates available from GenBank. Analyses showed the white-tailed deer isolate to be identical with the C. parvum cattle genotype; however, the caribou isolate represents a new genotype closely related to C. serpentis, C. muris, and C. andersoni. Giardia sp. was not detected in any of the caribou samples nor was Cryptosporidium sp. or Giardia sp. detected in any of the 42 moose (Alces alces) samples examined.
Siefker
C
C
College of Veterinary Medicine, Mississippi State University, Mississippi State 39762, USA. siefker@cvm.msstate.edu
Rickard
L G
LG
Pharr
G T
GT
Simmons
J S
JS
O'Hara
T M
TM
D000413:Alaska; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D004285:Dogs; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012053:Reindeer / Q000469:parasitology*; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D006360:Heat-Shock Proteins; D012337:RNA, Ribosomal, 18S