title: Telomerase activity and its correlation with the proliferative potential of bone marrow in aplastic anemia in children.
abstract: OBJECTIVE
  To explore telomerase activity and its association with the proliferative potential of hematopoietic stem cells in bone marrow (BM) in patients with aplastic anemia (AA).
  
  
  METHODS
  Telomerase activity of mononuclear cells separated from BM was determined with the TRAPeze kit. BM specimens from 22 cases with AA and 7 normal controls were included. SPSS10.0 was applied to analyze data derived from telomerase activity and colony-forming unit-granulocyte and monocyte.
  
  
  RESULTS
  The median telomerase activity level of BM in AA was 2-fold higher than that in normal controls. There was an inverse correlation in AA between telomerase activity and colony-forming unit (r = 0.78, p < 0.05). BM of chronic AA expressed higher telomerase than that of acute AA.
  
  
  CONCLUSIONS
  Telomerase activity in AA was increased and may be the result of the negative feedback of hematopoietic potential. Telomerase activity varied between the subtypes of AA. Telomerase activity was conversely correlated with the proliferative potential of BM in AA.
authors.affiliation: Department of Hematology, Xinhua Hospital, Shanghai Second Medical University, Shanghai, China. jianbinshen@etang.com
authors.forename: Jian Bin
authors.initials: JB
authors.lastname: Shen
authors.forename: Jing Yang
authors.initials: JY
authors.lastname: Tang
authors.forename: Jin Cai
authors.initials: JC
authors.lastname: Zhao
authors.forename: Ci
authors.initials: C
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Yao Ping
authors.initials: YP
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000741:Anemia, Aplastic / Q000201:enzymology* / Q000473:pathology; D015415:Biomarkers; D001853:Bone Marrow / Q000201:enzymology / Q000473:pathology*; D001854:Bone Marrow Cells / Q000201:enzymology*; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D018450:Disease Progression; D005260:Female; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006801:Humans; D007223:Infant; D008297:Male; D009000:Monocytes / Q000201:enzymology*; D019098:Telomerase / Q000032:analysis*
chemical_list: D015415:Biomarkers; D019098:Telomerase