cDNA macroarray analysis of gene expression in synoviocytes stimulated with TNFalpha.
Gene expression of synoviocytes stimulated with tumor necrosis factor-alpha (TNFalpha) was studied by macroarray analysis to elucidate the cellular response and identify new biological functions of known and unknown genes. 10035 cDNA clones were used to make cDNA macroarrays of representative genes. Synoviocytes expressed large amounts of fibronectin and collagen mRNA. Statistical analysis of the macroarray data revealed 26 genes, including six new genes, which underwent significant alteration of gene expression in response to TNFalpha stimulation. These findings suggest that the synoviocyte response to TNFalpha stimulation forms the basis of development of various aspects of the pathophysiology of rheumatoid arthritis.
Sugiyama
Tomoyasu
T
Helix Research Institute, Inc., 1532-3 Yana, Kisarazu-shi, Chiba, Japan. tsugiyama@kyowa.co.jp
Ishii
Shizuko
S
Yamamoto
Jun ichi
Ji
Irie
Ryotaro
R
Saito
Kaoru
K
Otuki
Tetsuji
T
Wakamatsu
Ai
A
Suzuki
Yuzuru
Y
Hio
Yuri
Y
Ota
Toshio
T
Nishikawa
Tetsuo
T
Sugano
Sumio
S
Masuho
Yasuhiko
Y
Isogai
Takao
T
D000595:Amino Acid Sequence; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D017386:Sequence Homology, Amino Acid; D013583:Synovial Membrane / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D005353:Fibronectins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D003094:Collagen