FCLA chemiluminescence from sonodynamic action in vitro and in vivo.
In this work, the chemiluminescence method was engaged for the first time to detect the active oxygen species during sonodynamic action in vitro and in vivo. We used FCLA (3,7-dihydro-6-[4-[2-[N'-(5-fluoresceinyl)thioureido]-ethoxy]phenyl]-2-methylimidazo[1,2-a]pyrazin-3-one sodium salt), which can selectively react with singlet oxygen ((1)O(2)) or superoxide anion (O(2)(-)) to emit photons, to detect in real time oxygen free radical formation in the sonosensitization of hematoporphyrin derivative. The results show that (1)O(2) is involved in the sonosensitization. In in vivo experiments, a tumor-imaging method by sonodynamic chemiluminescence detection was established. This method could have potential applications in clinics for tumor diagnosis.
He
Yonghong
Y
Institute of Laser Life Science, South China Normal University, Guangzhou 510631 China.
Xing
Da
D
Yan
Guihong
G
Ueda
Ken ichi
Ki
D005452:Fluoresceins; D016166:Free Radical Scavengers / Q000032:analysis; D006801:Humans; D007093:Imidazoles; D008163:Luminescent Measurements; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology*; D017785:Photons; D017382:Reactive Oxygen Species / Q000032:analysis*; D012680:Sensitivity and Specificity; D026082:Singlet Oxygen / Q000032:analysis; D013481:Superoxides / Q000032:analysis
D005452:Fluoresceins; D016166:Free Radical Scavengers; D007093:Imidazoles; D017382:Reactive Oxygen Species; D013481:Superoxides; C080213:3,7-dihydro-6-(4-(2-(N'-(5-fluoresceinyl)thioureido)ethoxy)phenyl)-2-methylimidazo-(1,2-a)pyrazin-3-one; D026082:Singlet Oxygen