title: Localization of cAMP-dependent protein kinase in the actin and microtubule cytoskeletons in mouse hippocampal neurons.
abstract: Cyclic adenosine monophosphate-dependent protein kinase (PKA) is involved in various biological functions in neurons. To investigate the subcellular localization of PKA, we stained cultured hippocampal neurons with anti-PKA catalytic subunit antisera. PKA catalytic subunit colocalized with microtubules (MTs) in dendrites as well as with the actin filaments (F-actin) in growth cones. After treatment with cytochalasin B, the colocalization of PKA catalytic subunits with MTs was enhanced, whereas the colocalization with F-actin was suppressed. This result indicates that PKA is anchored to the actin and MT cytoskeletons, and disruption of F-actin releases PKA to the cytoplasm, which then leads to an increase in the amount of PKA in MT domains in the neuron.
authors.affiliation: Laboratory of Nuclear Signaling, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sato
authors.forename: Reiko
authors.initials: R
authors.lastname: Sato-Harada
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Takano
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Kato
authors.forename: Sakura
authors.initials: S
authors.lastname: Saburi
authors.forename: Akihiro
authors.initials: A
authors.lastname: Harada
mesh_terms: D000199:Actins / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000201:enzymology*; D003712:Dendrites / Q000201:enzymology; D020439:Growth Cones / Q000201:enzymology; D006624:Hippocampus / Q000201:enzymology*; D051379:Mice; D008870:Microtubules / Q000201:enzymology*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology; D014018:Tissue Distribution
chemical_list: D000199:Actins; D003571:Cytochalasin B; D017868:Cyclic AMP-Dependent Protein Kinases