title: Extraction of thymol, eucalyptol, menthol, and camphor residues from honey and beeswax. Determination by gas chromatography with flame ionization detection.
abstract: A gas chromatographic method to determine thymol, eucalyptol (cineole), menthol and camphor residues in honey and beeswax is proposed. To isolate the compounds, three methods involving liquid-liquid extraction with methylene chloride, distillation, or solid-phase extraction on octadecylsilica cartridges can be used. The GC separation is carried out on a 60 m x 0.53 mm Stabilwax DA capillary column, using a flame ionization detector. The method is applied to the analysis of natural honey and also honey and beeswax samples from beehives treated with the above compounds.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain. mjdnozal@qa.uva.es
authors.forename: M J
authors.initials: MJ
authors.lastname: Nozal
authors.forename: J L
authors.initials: JL
authors.lastname: Bernal
authors.forename: J J
authors.initials: JJ
authors.lastname: Jiménez
authors.forename: M J
authors.initials: MJ
authors.lastname: González
authors.forename: M
authors.initials: M
authors.lastname: Higes
mesh_terms: D002164:Camphor / Q000302:isolation & purification*; D002849:Chromatography, Gas / Q000379:methods*; D003511:Cyclohexanols / Q000302:isolation & purification*; D000077591:Eucalyptol; D006722:Honey / Q000032:analysis*; D008610:Menthol / Q000302:isolation & purification*; D039821:Monoterpenes; D012015:Reference Standards; D015203:Reproducibility of Results; D013729:Terpenes / Q000302:isolation & purification*; D013943:Thymol / Q000302:isolation & purification*; D014885:Waxes / Q000032:analysis*
chemical_list: D003511:Cyclohexanols; D039821:Monoterpenes; D013729:Terpenes; D014885:Waxes; D008610:Menthol; C038228:beeswax; D013943:Thymol; D002164:Camphor; D000077591:Eucalyptol