[Glomerular morphologic protection after acute ischemia through the administration of Surgiran: (PGE1 and Alfadex, glucose cyclic oligomer). Morphometric study].
OBJECTIVE
To quantify the morphologic protection of PGE1 on renal glomerulus following normothermic acute renal ischemia.


METHODS
This experimental study was carried out in 48 adult male Wistar rats. A simple right nephrectomy was performed in all rats. The rats were divided into two groups: one group was perfused with PGE1 and the other group with saline solution immediately after acute renal ischemia, which was produced by non-traumatic vascular clamping. The ischemia times were 15 and 60 minutes. The recovery period ranged from 24 hours to 7 days. After this period the animals were anesthetized and sacrificed. Histological and morphometric analyses of the right kidneys of the control group and the left kidneys of the study group were performed.


RESULTS
The mortality was 31% (18.5% for the group perfused with PGE1 and 11.4% for the group perfused with saline solution). Kidneys of rats perfused with saline solution weighed more than the kidneys of rats perfused with PGE1 (1.771 +/- 0.455 and 1.55 +/- 0.34, respectively). Acute tubular necrosis was observed after 60 minutes' ischemia and was more evident in the saline than in the PGE1 group. The morphometric study showed no significant differences between the control (normal) and the PGE1 group for glomerular diameter (p < or = 0.101), sphericity factor (p < or = 0.239), glomerular perimeter (p < or = 0.092) and glomerular volume (p < or = 0.059). However, significant differences were found between the control and the saline perfusion group for area (p < or = 0.000), diameter (p < or = 0.000), perimeter (p < or = 0.000) and volume (p < or = 0.000).


CONCLUSIONS
Kidney weight after ischemia increased more in the saline than in the PGE1-treated group since the edema in the renal parenchyma is attenuated by the anti-inflammatory and cytoprotective effects of PGE1. The morphometric study showed that in comparison to saline, PGE1 exerts a cytoprotective effect, although it is not considerable.
Conde Redondo
Consuelo
C
Servicio de Urología, Hospital Universitario Río Hortega, Valladolid, España. cconde@airtel.net
Herreros Rodríguez
Vicente
V
Rodríguez-Toves
Luis Antonio
LA
Estabanez Zarranz
Javier
J
Martínez Sagarra
José María
JM
Vaquero
Carlos
C
D000208:Acute Disease; D000527:Alprostadil / Q000627:therapeutic use*; D000818:Animals; D003505:Cyclodextrins / Q000627:therapeutic use*; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D007511:Ischemia / Q000517:prevention & control*; D007668:Kidney / Q000033:anatomy & histology / Q000098:blood supply*; D007678:Kidney Glomerulus / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D047391:alpha-Cyclodextrins
D003505:Cyclodextrins; D047391:alpha-Cyclodextrins; C051518:OP 41483-alpha-cyclodextrin; D011464:Epoprostenol; D000527:Alprostadil
Protección morfológica glomerular tras isquemia aguda mediante la administración de Surgiran: (PGE1 y Alfadex, oligómero cíclico de la glucosa). Estudio morfométrico.