[Population study of HLA DQA1, LDLR, GYPA, HBGG, D7S8, and GC loci in Caucasians living in the Ural region of Russia].
Allele frequencies have been determined for 6 tested locuses. The distribution of HLA DQA1 gene and locuses of Polymarker kit (Perkin Elmer, USA) for population studies conformed to the Hardy-Weinberg equilibrium (HWE). Inequilibrium for linkage for all possible locus pairs was tested by an accurate test. No statistically significant inequilibrium was detected. Paired comparison of allele frequencies of tested locuses of the Ural population with other Caucasoid populations showed homogeneity for GYPA, HBGG, D7S8, and HLA DQA1 locuses. Significant differences for 1 and 2 of the 4 compared pairs were detected for LDLR and GC locuses, respectively. The total discrimination potential for 6 tested locuses was 0.9995. Our population study showed that high informative value of the tested genetic markers and conformity to HWE make them a useful tool for forensic genetic studies in the Ural Caucasoid population. Preliminary assessment of the efficiency of PCR of 6 studied locuses by capillary electrophoresis is highly effective in typing of DNA samples isolated from material evidences with negligible content of biological material with presumably high degree of degradation and for evaluating the positive and negative amplification controls.
Pushkarev
V P
VP
Novikov
P I
PI
D005554:Forensic Medicine; D005828:Genetics, Population; D005936:Glucans / Q000235:genetics*; D006162:Guanylate Cyclase / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D006801:Humans; D016133:Polymerase Chain Reaction; D011973:Receptors, LDL / Q000235:genetics*; D012426:Russia; D044465:White People / Q000235:genetics*
D005936:Glucans; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D011973:Receptors, LDL; C042659:hexa(beta-glucopyranosyl)glucitol; D006162:Guanylate Cyclase
Populiatsionnoe issledovanie lokusov HLA DQA1, LDLR, GYPA, HBGG, D7S8 i GC u kavkazoidov ural'skogo regiona Rossii.