Inhibition of bacterial pyridoxal-depending enzymes by (aminooxy)-acetic acid improves selective 15N isotope labeling of bacterially expressed protein.
Lopukhov
Leonid V
LV
Kazan Institute of Biochemistry and Biophysics, Russia. leonid_lopuhov@mail.ru
Ponomareva
Anna A
AA
Yagodina
Lilia O
LO
D000625:Aminooxyacetic Acid / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009587:Nitrogen Isotopes / Q000378:metabolism; D011730:Pyridoxal / Q000378:metabolism*; D000637:Transaminases / Q000037:antagonists & inhibitors* / Q000378:metabolism
D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D009587:Nitrogen Isotopes; D000625:Aminooxyacetic Acid; C057438:barstar protein, Bacillus amyloliquefaciens; D011730:Pyridoxal; D000637:Transaminases