title: Identification of the Peptides that Bind to Tyrosine Kinase Receptor EphB2 by Phage Display.
abstract: The gene encoding the Ig-like domain of tyrosine protein kinase receptor EphB2 was cloned into the expressing vector pET28a. Under induction with IPTG, the positive strain expressed the fusion protein with a hexahistidine tail on the N-terminal. The protein was purified under denaturing conditions using metal chelate chromatography. The purity was up to 94%. The purified-protein-coated ELISA plate was used as target to screen recombinant phages able to bind onto it, and after three rounds of affinity screening, 19 phages that could bind specifically with EphB2 were isolated from a random phage-displayed seven-peptide library. The peptide sequences of the positive phage clones were analyzed.
authors.affiliation: Department of Biochemistry and Molecular Biology, the Fourth Military Medical University, Xi'an 710032, China. bioyao@fmmu.edu.cn
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Zhang
authors.forename: Li-Bo
authors.initials: LB
authors.lastname: Yao
authors.forename: Jiong
authors.initials: J
authors.lastname: Han
authors.forename: Xin-Ping
authors.initials: XP
authors.lastname: Liu
authors.forename: Jing-Tian
authors.initials: JT
authors.lastname: Han
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Nie
authors.forename: Cheng-ZHi
authors.initials: CZ
authors.lastname: Su