A molecular approach for early monitoring of decay basidiomycetes in wood chips.
A DNA-based method for the early detection and identification of decay basidiomycetes on wood chips is described. From 26 species of wood-decaying basidiomycetes and 20 species of wood-inhabiting ascomycetes, DNA fragments containing the internal transcribed spacers, 5.8S and partial 28S rDNA were amplified by PCR using the ITS1-F-NL2 rDNA primer pair and analysed through sequencing and restriction digestion. A decay basidiomycete-specific restriction fragment length polymorphism (RFLP) pattern was generated by DraI. Using this PCR-RFLP method the detection of decay fungi is possible 4 days after the inoculation of the wood chips with either a single culture or mixed species.
Adair
Solveig
S
Chair of Forest Products Biotechnology, Department of Wood Science, University of British Columbia, 4041-2424 Main Mall, V6T 1Z4, Vancouver, BC, Canada.
Kim
Seong Hwan
SH
Breuil
Colette
C
D001487:Basidiomycota / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014197:Trees / Q000382:microbiology*; D014934:Wood
D017931:DNA Primers; D004271:DNA, Fungal; D004275:DNA, Ribosomal