title: The regional distribution and relative frequency of gastrointestinal endocrine cells in SHK-1 hairless mice: an immunohistochemical study.
abstract: The regional distributions and relative frequencies of some gastrointestinal endocrine cells in the eight portions (fundus, pylorus, duodenum, jejunum, ileum, caecum, colon and rectum) of the gastrointestinal tract of SKH-1 hairless mice were investigated using immunohistochemical methods and seven types of specific antisera against somatostatin, serotonin, glucagon, cholecystokinin (CCK)-8, secretin, pancreatic polypeptide (PP) and gastrin. In this study, somatostatin-, serotonin-, glucagon-, CCK-8-, secretin- and gastrin-immunoreactive (IR) cells were identified. Most of these IR cells in the intestinal portion were generally spherical or spindle-shaped (open-type cell) while cells that were round in shape (close-type cell) were occasionally found in the stomach regions. Their relative frequencies were varied according to each portion of gastrointestinal tract. Somatostatin-IR cells were found throughout the gastrointestinal tract except for the large intestine. Serotonin-IR cells were detected throughout the whole gastrointestinal tract and were the most predominant endocrine cell types in this species of mouse. Glucagon-IR cells were restricted to the fundus, occurring rarely. CCK-8-IR cells were observed in the pylorus, duodenum and jejunum with frequencies that were numerous, moderate and few, respectively. Peculiarly, secretin-IR cells were demonstrated in the whole intestinal tract with either few or rare frequencies. Gastrin-IR cells were restricted to the pylorus and were numerous. However, no PP-IR cells were found in this study. In conclusion, some peculiar distributional patterns of gastrointestinal endocrine cells were found in SKH-1 hairless mouse.
authors.affiliation: Pharmacology & Toxicology Laboratory, Dong-Wha Pharmaceutical Industrial Co, Anyang 430-017, Korea.
authors.forename: S-K
authors.initials: SK
authors.lastname: Ku
authors.forename: J-H
authors.initials: JH
authors.lastname: Lee
authors.forename: H-S
authors.initials: HS
authors.lastname: Lee
authors.forename: K-D
authors.initials: KD
authors.lastname: Park
mesh_terms: D000818:Animals; D004064:Digestive System / Q000737:chemistry / Q000166:cytology*; D019858:Enteroendocrine Cells / Q000737:chemistry / Q000166:cytology*; D005260:Female; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D008297:Male; D051379:Mice / Q000033:anatomy & histology*; D008812:Mice, Hairless