title: Validation of the Coulter AcT Diff hematology analyzer for analysis of blood of common domestic animals.
abstract: The objective of this study was to compare and assess the agreement between the Coulter AcT Diff hematology analyzer (CAD) and the Bayer Technicon H1 (H1) using blood samples from 391 animals of 4 species. The H1 has been used in veterinary laboratories for many years. Recently, Coulter modified the CAD and added veterinary software for hematologic analysis of feline, canine, and equine samples. A comparison of hemograms from dogs, cats, horses, and cattle was made using EDTA-anticoagulated blood samples. Both instruments were calibrated using human blood products. Performance characteristics were excellent for most values. The exceptions were MCV in canine samples (concordance correlation of.710), platelet counts for feline and equine samples (.258 and.740, respectively), feline and bovine WBC counts (.863 and.857, respectively), and bovine hemoglobin (.876).
authors.affiliation: Animal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, ON N1H 6R8, Canada. bhoff@lsd.uoguelph.ca
authors.forename: Hilary
authors.initials: H
authors.lastname: Dawson
authors.forename: Brent
authors.initials: B
authors.lastname: Hoff
authors.forename: Evert
authors.initials: E
authors.lastname: Grift
authors.forename: Harold
authors.initials: H
authors.lastname: Tvedten
authors.forename: Mohammed
authors.initials: M
authors.lastname: Shoukri