title: Antimutagenicity of WR-1065 in L5178Y cells exposed to accelerated (56)Fe ions.
abstract: The ability of the aminothiol WR-1065 [N-(2-mercaptoethyl)-1,3-diaminopropane] to protect L5178Y (LY) cells against the cytotoxic and mutagenic effects of exposure to accelerated (56)Fe ions (1.08 GeV/nucleon) was determined. It was found that while WR-1065 reduced the mutagenicity in both cell lines when it was present during the irradiation, the addition of WR-1065 after the exposure had no effect on the mutagenicity of the radiation in either cell line. No marked protection against the cytotoxic effects of exposure to (56)Fe ions was provided by WR-1065 when added either during or after irradiation in either cell line. We reported previously that WR-1065 protected the LY-S1 and LY-SR1 cell lines against both the cytotoxicity and mutagenicity of X radiation when present during exposure, but that its protection when administered after exposure was limited to the mutagenic effects in the radiation-hypersensitive cell line, LY-S1. The results indicate that the mechanisms involved differ in the protection against cytotoxic compared to mutagenic effects and in the protection against damage caused by accelerated (56)Fe ions compared to X radiation.
authors.affiliation: Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4942, USA. hhe@po.cwru.edu
authors.forename: H H
authors.initials: HH
authors.lastname: Evans
authors.forename: T E
authors.initials: TE
authors.lastname: Evans
authors.forename: M F
authors.initials: MF
authors.lastname: Horng
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects; D007504:Iron Radioisotopes / Q000494:pharmacology*; D007940:Leukemia L5178 / Q000235:genetics*; D008624:Mercaptoethylamines / Q000494:pharmacology*; D009154:Mutation / Q000187:drug effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D016587:Antimutagenic Agents; D007504:Iron Radioisotopes; D008624:Mercaptoethylamines; D011837:Radiation-Protective Agents; C020174:N-(2-mercaptoethyl)-1,3-diaminopropane; D004247:DNA