title: Human leucocyte antigen-Cw-specific cytotoxic T lymphocytes generated from naive cord blood used for cord blood stem cell transplantation.
abstract: Human leucocyte antigen (HLA)-Cw-reactive cytotoxic T lymphocytes (CTL) were generated from cord blood (CB) lymphocytes of two cases used for cord blood stem cell transplantation (CBSCT). In both cases, the CTL were cytotoxic against the patient's leukaemic cells, as well as the patient's Epstein-Barr virus (EBV)-lymphoblastoid cell line (EBV-LCL) and phytohaemagglutinin blasts, and the cytotoxicity was blocked by anti-HLA-class I monoclonal antibodies. In the first case, the CTL recognized Cw 3 (Cw 9 and Cw 10)-positive EBV-LCL, while in the second case, the CTL recognized Cw1 and/or Cw7. These cases suggest that CB T cells may be competent enough for generating CTL to induce a graft-versus-leukaemia effect and/or graft-versus-host disease in patients with CBSCT and that the mismatching of Cw antigens between patient and CB may be related to the outcome of CBSCT.
authors.affiliation: Department of Paediatrics, Nagoya City University Medical School, 1-Kawasumi, Mizuho-cho, Nagoya 467-8601, Japan. yazaki@med.nagoya-cu.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Yazaki
authors.forename: Toshitada
authors.initials: T
authors.lastname: Takahashi
authors.forename: Keigo
authors.initials: K
authors.lastname: Mizutani
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Wakiguchi
authors.forename: Masami
authors.initials: M
authors.lastname: Inoue
authors.forename: Keisei
authors.initials: K
authors.lastname: Kawa
authors.forename: Koji
authors.initials: K
authors.lastname: Kato
authors.forename: Toro
authors.initials: T
authors.lastname: Kato
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Saito
authors.forename: Hajime
authors.initials: H
authors.lastname: Togari
mesh_terms: D000208:Acute Disease; D002469:Cell Separation; D002675:Child, Preschool; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005312:Fetal Blood / Q000276:immunology*; D006087:Graft vs Host Reaction / Q000276:immunology; D020544:Graft vs Leukemia Effect / Q000276:immunology; D015236:HLA-C Antigens / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D004854:Herpesvirus 4, Human; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology / Q000821:virology; D007951:Leukemia, Myeloid / Q000276:immunology / Q000601:surgery; D008297:Male; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000601:surgery; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D015236:HLA-C Antigens; C038653:HLA-C*03 antigen; C040178:HLA-C*70 antigen; C047431:HLA-Cw1 antigen