title: Hard X-ray microprobe studies of chromium(VI)-treated V79 Chinese hamster lung cells: intracellular mapping of the biotransformation products of a chromium carcinogen.
abstract: The uptake of carcinogenic and mutagenic Cr compounds and the intracellular distribution of their biotransformation products in V79 Chinese hamster lung cells were studied by synchrotron-radiation-induced X-ray emission (SRIXE). SRIXE analysis was performed on whole cells that had been treated with either Cr(III) or Cr(V) 1,10-phenanthroline complexes, or Cr(VI). The high spatial resolution (0.3 microm) and elemental sensitivity (~10(-15) g Cr/cell) of the technique provided detailed maps of Cr and other cellular elements in thin sections prepared from Cr(VI)-treated cells. The Cr carcinogen concentrated in P-rich regions corresponding to the nucleus, as well as other areas of the cell that are likely to correspond to organelles. This is the first study that has enabled the determination of the localization of the biotransformation products of Cr(VI) carcinogens in a target lung cell.
authors.affiliation: Centre for Heavy Metals Research, School of Chemistry, University of Sydney, NSW 2006, Australia.
authors.forename: Carolyn T
authors.initials: CT
authors.lastname: Dillon
authors.forename: Peter A
authors.initials: PA
authors.lastname: Lay
authors.forename: Brendan J
authors.initials: BJ
authors.lastname: Kennedy
authors.forename: Anton P J
authors.initials: AP
authors.lastname: Stampfl
authors.forename: Zhonghou
authors.initials: Z
authors.lastname: Cai
authors.forename: Peter
authors.initials: P
authors.lastname: Ilinski
authors.forename: William
authors.initials: W
authors.lastname: Rodrigues
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Legnini
authors.forename: Barry
authors.initials: B
authors.lastname: Lai
authors.forename: Jörg
authors.initials: J
authors.lastname: Maser
mesh_terms: D000818:Animals; D001711:Biotransformation; D015197:Carcinogenicity Tests; D002460:Cell Line; D002857:Chromium / Q000493:pharmacokinetics* / Q000633:toxicity; D017608:Chromium Compounds / Q000493:pharmacokinetics* / Q000633:toxicity; D006224:Cricetinae; D003412:Cricetulus; D004577:Electron Probe Microanalysis / Q000295:instrumentation / Q000379:methods; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014961:X-Ray Diffraction
chemical_list: D017608:Chromium Compounds; D002857:Chromium