Overexpression of extracellular superoxide dismutase decreases lung injury after exposure to oil fly ash.
The mechanism of tissue injury after exposure to air pollution particles is not known. The biological effect has been postulated to be mediated via an oxidative stress catalyzed by metals present in particulate matter (PM). We utilized a transgenic (Tg) mouse model that overexpresses extracellular superoxide dismutase (EC-SOD) to test the hypothesis that lung injury after exposure to PM results from an oxidative stress in the lower respiratory tract. Wild-type (Wt) and Tg mice were intratracheally instilled with either saline or 50 microg of residual oil fly ash (ROFA). Twenty-four hours later, specimens were obtained and included bronchoalveolar lavage (BAL) and lung for both homogenization and light histopathology. After ROFA exposure, EC-SOD Tg mice showed a significant reduction in BAL total cell counts (composed primarily of neutrophils) and BAL total protein compared with Wt. EC-SOD animals also demonstrated diminished concentrations of inflammatory mediators in BAL. There was no statistically significant difference in BAL lipid peroxidation; however, EC-SOD mice had lower concentrations of oxidized glutathione in the BAL. We conclude that enhanced EC-SOD expression decreased both lung inflammation and damage after exposure to ROFA. This supports a participation of oxidative stress in the inflammatory injury after PM exposure rather than reflecting a response to metals alone.
Ghio
Andrew J
AJ
National Health and Environmental Effects Research Laboratory, Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA.
Suliman
Hagir B
HB
Carter
Jacqueline D
JD
Abushamaa
Amir M
AM
Folz
Rodney J
RJ
D000393:Air Pollutants / Q000494:pharmacology*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000276:immunology; D002244:Carbon / Q000494:pharmacology*; D060729:Coal Ash; D005609:Free Radicals / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D005978:Glutathione / Q000032:analysis; D008171:Lung Diseases / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D009504:Neutrophils / Q000276:immunology; D018384:Oxidative Stress / Q000187:drug effects; D052638:Particulate Matter; D013482:Superoxide Dismutase / Q000235:genetics*; D014639:Vanadium / Q000494:pharmacology
D000393:Air Pollutants; D060729:Coal Ash; D005609:Free Radicals; D052638:Particulate Matter; D014639:Vanadium; D002244:Carbon; D013482:Superoxide Dismutase; D005978:Glutathione