Genetic transformation of cauliflower (Brassica oleracea var. botrytis) by direct DNA uptake into mesophyll protoplasts.
Mesophyll protoplasts of Brassica oleracea var. botrytis were successfully transformed using polyethylene glycol (PEG). The success of plant transformation depended on both gene transfer and plant regeneration. Parameters, such as PEG and vector concentrations and heat shock conditions were tested in experiments on transient expression of the beta-glucuronidase (EC 3.2.1.31) gene and the most suitable conditions for DNA uptake were determined. Two antibiotic resistance marker genes for neomycin phosphotransferase (EC 2.7.1.95) and hygromycin phosphotransferase (EC 2.7.1.104), and three vector plasmids with different lengths were used to obtain stable transformants.
Radchuk
Volodymyr V
VV
Institute of Horticultural Crops, Federal Centre for Breeding Research on Cultivated Plants, Neuer Weg 22/23, D-06484 Quedlinburg, Germany Present address: Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany Corresponding author, e-mail: e.klocke@bafz.de
Ryschka
Ulrich
U
Schumann
Günter
G
Klocke
Evelyn
E