Transepithelial transport of poly(amidoamine) dendrimers across Caco-2 cell monolayers.
The objective of this study was to investigate the influence of physiochemical parameters (such as size, molecular weight, molecular geometry, and number of surface amine groups) of poly (amidoamine) (PAMAM) dendrimers, on their permeability across Caco-2 cell monolayers. The permeability of a series of PAMAM dendrimers, generations 0-4 (G0-G4), was investigated across Caco-2 cell monolayers in both the apical to basolateral (AB) and basolateral to apical (BA) directions. The influence of PAMAM dendrimers on the integrity, paracellular permeability, and viability of Caco-2 cell monolayers was also monitored by measuring the transepithelial electrical resistance (TEER), mannitol permeability, and leakage of lactate dehydrogenase (LDH) enzyme, respectively. G0, G1 and G2 demonstrated similar AB permeabilities, which were moderate several fold higher than the AB permeability of higher generations. The AB and BA permeability of G0-G4 typically increased with the increase in donor concentration and incubation time. Permeability values are not reported at generations, concentrations or incubation times that the dendrimers were toxic to Caco-2 cells. TEER values decreased and mannitol permeability increased as a function of donor concentration, incubation time, and generation number. LDH results for G3 and G4 indicate that Caco-2 cell viability was reduced with increasing donor concentration, incubation time, and generation number. The appreciable permeability of G0-G2, coupled with their nontoxic effects on Caco-2 cells, suggest their potential as water-soluble polymeric drug carriers for controlled oral drug delivery.
El-Sayed
Mohamed
M
University of Maryland School of Pharmacy, Department of Pharmaceutical Sciences, 20 N. Pine Street, Baltimore, MD 21201, USA.
Ginski
Mark
M
Rhodes
Christopher
C
Ghandehari
Hamidreza
H
D001672:Biocompatible Materials / Q000737:chemistry / Q000493:pharmacokinetics* / Q000633:toxicity; D001693:Biological Transport, Active; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability; D050091:Dendrimers; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics; D017097:Electric Impedance; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007408:Intestinal Absorption / Q000502:physiology; D011073:Polyamines / Q000737:chemistry / Q000493:pharmacokinetics* / Q000633:toxicity
D001672:Biocompatible Materials; D050091:Dendrimers; D004337:Drug Carriers; C104700:PAMAM Starburst; D011073:Polyamines