Taenia solium: identification of specific antibody binding regions of metacestode 10-kDa protein.
Taenia solium neurocysticercosis (NCC) represents one of the major public health problems associated with several neurological manifestations worldwide. We previously identified a recombinant 10-kDa protein of T. solium metacestode (CyDA) specific to active NCC. Immunoblottings with sera from active NCC patients and from animals experimentally infected with larval T. solium (pig), T. saginata (pig), T. asiatica (pig), and T. crassiceps (mouse) strongly recognized CyDA, while sera from patients infected only with adult worms did not. Mapping of antigenic sites using deletion mutants revealed that amino acids (aa) residues 30-34, Asn-Met-Thr-Val-Met (NMTVM), reacted only with sera from active stage T. solium cysticercosis cases. Recognition of CyDA aa 30-34 resided almost exclusively in the IgG4 isotype. Competitive immunoprecipitation with synthetic peptides confirmed the specificity of anti-sera for this penta-peptide. These results demonstrated that aa residues NMTVM in CyDA comprise the core sequence for an active stage NCC-related antigenic determinant. ligand binding protein, HLBP; Cyst fluid, CF; Pooled serum of 10 active NCC patients, serum-pool.
Chung
Joon-Yong
JY
Section of Molecular Parasitology, Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, 440-746, South Korea.
Yun
Doo-Hee
DH
Eom
Keeseon S
KS
Kang
Shin-Yong
SY
Kong
Yoon
Y
Cho
Seung-Yull
SY
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology*; D003552:Cysticercus / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D015801:Helminth Proteins / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D020019:Neurocysticercosis / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology*; D011233:Precipitin Tests; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013552:Swine
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000939:Epitopes; D015801:Helminth Proteins