Biochemical properties of the human REV1 protein.
It has been proposed that the REV1 protein plays an important role in the induced-mutagenesis pathway. We show that purified REV1 protein inserts dCMP opposite template G, A, T and C, and dGMP and dTMP opposite template G in the presence of magnesium, while in the presence of manganese the specificity for dCMP was found to be relaxed and the REV1 protein acquired the ability to insert dCMP, dGMP, dAMP and dTMP opposite templates G, A, T, and C. Kinetic analysis provided evidence for high affinity for dCTP with template G, suggesting that the REV1 protein is specialized for dCTP and template G.
Masuda
Yuji
Y
Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshoima 734-8553, Japan.
Kamiya
Kenji
K
D001483:Base Sequence; D002413:Cations, Divalent / Q000494:pharmacology; D003843:Deoxycytidine Monophosphate / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D009713:Nucleotidyltransferases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D000099270:Y-Family DNA Polymerases
D002413:Cations, Divalent; D003843:Deoxycytidine Monophosphate; D009687:Nuclear Proteins; D009713:Nucleotidyltransferases; D011993:Recombinant Fusion Proteins; C429004:REV1 protein, human; D000099270:Y-Family DNA Polymerases