Production of cycloisomaltooligosaccharides from dextran using enzyme immobilized in multilayers onto porous membranes.
Anion-exchange porous hollow-fiber membranes with a thickness of about 1.2 mm and a pore size of about 0.30 microm were used as a supporting matrix to immobilize cycloisomaltooligosaccharide glucanotransferase (CITase). CITase was immobilized to the membrane via anion-exchange adsorption and by subsequent enzymatic cross-linking with transglutaminase, the amount of which ranged from 3 to 110 mg per gram of the membrane. The degree of enzyme multilayer binding was equivalent to 0.3-9.8. Dextran, as the substrate, was converted into seven- to nine-glucose-membered cycloisomaltooligosaccharides (CI-7, -8, and -9) at a maximum yield of 28% in weight at a space velocity of 10 per hour during the permeation of 2.0% (w/w) dextran solution across the CITase-immobilized porous hollow-fiber membrane. The yield of CIs increased with increasing degree of CITase multilayering.
Kawakita
Hidetaka
H
Department of Materials Technology, Faculty of Engineering, Chiba University, Inage, Japan.
Sugita
Kazuyuki
K
Saito
Kyoichi
K
Tamada
Masao
M
Sugo
Takanobu
T
Kawamoto
Hiroshi
H
D000042:Absorption; D000837:Anion Exchange Resins; D003911:Dextrans / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D008567:Membranes, Artificial; D009844:Oligosaccharides / Q000096:biosynthesis*
D000837:Anion Exchange Resins; D003911:Dextrans; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D009844:Oligosaccharides