cDNA cloning and distribution of XEXT1, the Xenopus homologue of EXT1.
Hereditary Multiple Exostosis (EXT) is an autosomal dominant disorder. Here, we have isolated XEXT1, a Xenopus homologue of EXT1, as an ovary-enriched cDNA clone. The 2,598-bp XEXT1 cDNA had a single open reading frame encoding 735 amino acids. Quantitative RT-PCR analysis showed that transcripts of XEXT1 were present maternally and consumed prior to gastrulation. Zygotic expression of XEXT1 was not detected during late embryogenesis. In adult organs, XEXT1 was expressed intensely in bone and lung. Whole-mount in situ hybridization showed that maternally transcribed XEXT1 mRNAs were stored in the animal hemisphere, and were localized in the center of the cell during cleavage stages.
Katada
Tomohisa
T
Department of Life Science, School of Science and Technology, Kwansei Gakuin University, 2-1, Gakuen, Sanda, Hyogo, 669-1337, Japan.
Oogami
Shunji
S
Shima
Naoya
N
Kinoshita
Tsutomu
T
D000818:Animals; D036703:Blastula / Q000502:physiology; D017403:In Situ Hybridization; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics*; D009866:Oogenesis / Q000502:physiology; D009928:Organ Specificity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014981:Xenopus; D000099058:Exostosin 1
D017351:N-Acetylglucosaminyltransferases; D000099058:Exostosin 1