[Molecular portrait of human kidney carcinomas: the gene expression profiling of protein-tyrosine kinases and tyrosine phosphatases which controlled regulatory signals in the cells].
Hybridization with cDNA arrays was used to obtain expression profiles of 214 protein-tyrosine kinase, protein-tyrosine phosphatase, dual-specific phosphatase, and other genes for kidney carcinomas (KC) and normal kidney tissues of 34 patients and for seven carcinoma cell lines. Computer analysis revealed three clusters of genes coexpressed in KC. A proliferating-cell gene cluster included MET, VIM, MYC, TOP2A, PCNA, etc. A neoangiogenesis and blood-cell gene cluster included LCK, HCK, FGR, MMP9, CSFR1, VEGF, FLT1, and KDR. A cluster corresponding to normal, differentiated kidney cells included ERBB2 (HER2) for receptor protein-tyrosine kinase, several phosphatase genes (PTPRE, PTPRB, DUSP9), and EGF. The results suggested that MET, DUSP9, PCNA, TOP2A, and VIM may serve as diagnostic and prognostic markers in KC. Tubulin and topoisomerase II were assumed to be promising targets for cell proliferation inhibitors in KC.
Cheburkin
Iu V
IuV
Research Institute of Roentgenology and Radiology, Ministry of Health of the Russian Federation, St. Petersburg, 197758 Russia.
Kniazeva
T G
TG
Peter
S
S
Kniazev
Iu P
IuP
Karelin
M I
MI
Shkol'nik
M I
MI
Evtushenko
V I
VI
Hanson
K P
KP
Ullrich
A
A
Kniazev
P G
PG
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D001706:Biopsy; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D004268:DNA-Binding Proteins; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000378:metabolism*; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism; D000075223:Poly-ADP-Ribose Binding Proteins; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000235:genetics
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D018809:Proliferating Cell Nuclear Antigen; D014746:Vimentin; D011505:Protein-Tyrosine Kinases; D019859:Proto-Oncogene Proteins c-met; D010749:Phosphoprotein Phosphatases; D004250:DNA Topoisomerases, Type II; C000618811:TOP2A protein, human
Molekuliarnyĭ portret kartsinom pochki cheloveka, poluchennyĭ na osnove ékspressii protein-tirozin-kinaz i tirozin-fosfataz, kontroliruiushchikh peredachu reguliatornykh signalov v kletkakh.