Analysis of minichromosome maintenance proteins as a novel method for detection of colorectal cancer in stool.
Colorectal cancer is a common disease, and more reliable screening methods are needed for early detection. We aim to develop a non-invasive, stool-based assay that can identify colorectal cancer by detection of minichromosome maintenance protein 2 (MCM2) expression in colonocytes retrieved from the faecal surface. We devised a cell line model to investigate methods and conditions for optimum colonocyte retrieval. In our clinical evaluation study, MCM2-positive cells were retrieved from 37 of 40 patients with symptomatic colorectal cancer, but from none of 25 healthy control individuals. These results suggest that immunocytochemical analysis of retrieved colonocytes might enable accurate detection of colorectal cancer in stool.
Davies
R Justin
RJ
MRC Cancer Cell Unit, Hutchison/MRC Research Centre, Cambridge CB2 2XZ, UK.
Freeman
Alex
A
Morris
Lesley S
LS
Bingham
Sheila
S
Dilworth
Stephen
S
Scott
Ian
I
Laskey
Ronald A
RA
Miller
Richard
R
Coleman
Nicholas
N
D000230:Adenocarcinoma / Q000175:diagnosis*; D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000032:analysis*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000175:diagnosis*; D005243:Feces / Q000737:chemistry*; D006801:Humans; D064111:Minichromosome Maintenance Complex Component 2; D009687:Nuclear Proteins / Q000032:analysis*; D015203:Reproducibility of Results
D014408:Biomarkers, Tumor; D009687:Nuclear Proteins; D064111:Minichromosome Maintenance Complex Component 2