Lysophosphatidic acid inhibition of the accumulation of Pseudomonas aeruginosa PAO1 alginate, pyoverdin, elastase and LasA.
The pathogenesis of Pseudomonas aeruginosa is at least partially attributable to its ability to synthesize and secrete the siderophore pyoverdin and the two zinc metalloproteases elastase and LasA, and its ability to form biofilms in which bacterial cells are embedded in an alginate matrix. In the present study, a lysophospholipid, 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphate [also called monopalmitoylphosphatidic acid (MPPA)], which accumulates in inflammatory exudates, was shown to inhibit the extracellular accumulation of P. aeruginosa PAO1 alginate, elastase, LasA protease and the siderophore pyoverdin. MPPA also inhibited biofilm formation. The inhibitory effects of MPPA occur independently of rpoS expression and without affecting the accumulation of the autoinducers N-(3-oxododecanoyl) homoserine lactone and N-butyryl-L-homoserine lactone, and may be due, at least in part, to the ability of MPPA to bind divalent cations.
Laux
David C
DC
Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02881, USA1.
Corson
Joy M
JM
Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02881, USA1.
Givskov
Michael
M
Biocentrum, Bldg 301, Technical University of Denmark, DK-2800 Lyngby, Denmark2.
Hentzer
Morten
M
Biocentrum, Bldg 301, Technical University of Denmark, DK-2800 Lyngby, Denmark2.
Møller
Annette
A
Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02881, USA1.
Wosencroft
Kathleen A
KA
Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02881, USA1.
Olson
Joan C
JC
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC 29425, USA3.
Krogfelt
Karen A
KA
Department of Gastrointestinal Infections, Statens Serum Institut, DK 2300 Copenhagen, Denmark4.
Goldberg
Joanna B
JB
Department of Microbiology, University of Virginia, Health Sciences Center, Charlottesville, VA 22908, USA5.
Cohen
Paul S
PS
Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02881, USA1.
D000464:Alginates / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D020689:Exopeptidases / Q000096:biosynthesis / Q000378:metabolism; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008246:Lysophospholipids / Q000494:pharmacology*; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000378:metabolism*; D008670:Metals / Q000494:pharmacology; D019081:O Antigens / Q000096:biosynthesis / Q000378:metabolism; D009842:Oligopeptides; D010860:Pigments, Biological / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D017262:Siderophores / Q000378:metabolism; D012808:Sigma Factor / Q000096:biosynthesis / Q000235:genetics
D000464:Alginates; D001426:Bacterial Proteins; D006603:Hexuronic Acids; D008246:Lysophospholipids; D008670:Metals; D019081:O Antigens; D009842:Oligopeptides; D010860:Pigments, Biological; D017262:Siderophores; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; C042453:pyoverdin; D020723:Glucuronic Acid; D004492:Edetic Acid; D020689:Exopeptidases; D008666:Metalloendopeptidases; C066371:staphylolytic protease