A cloth strip bioreactor with immobilized glucoamylase.
Glucoamylase was immobilized on polyethylenimine (PEI)-coated cotton cloth by adsorption followed by cross-linking with 0.2% glutaraldehyde in the presence of starch. Optimal adsorption of the enzyme was seen when cloth treated with 2% PEI was contacted with the enzyme for 50 min. pH and temperature optima profiles were not changed appreciably on immobilization. However, the bound enzyme exhibited a higher thermal stability. The enzyme-bound cloth strips were used in a specially designed bioreactor for the continuous hydrolysis of starch. The reactor could be operated for over 21 days retaining about 70% of the original activity. An operational temperature of 45 degrees C was found to be optimal.
D'Souza
S F
SF
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. sfdsouza@apsara.barc.ernet.in
Kubal
B S
BS
D000327:Adsorption; D001671:Biochemistry / Q000379:methods*; D019149:Bioreactors; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry* / Q000378:metabolism; D003368:Gossypium / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D011094:Polyethyleneimine / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D003432:Cross-Linking Reagents; D011094:Polyethyleneimine; D005087:Glucan 1,4-alpha-Glucosidase