title: The absence of factor V Leiden mutation in Malays with recurrent spontaneous abortions.
abstract: OBJECTIVE
  The objectives of this study were to investigate the prevalence of factor V Leiden mutation in Malay women with recurrent spontaneous abortion and to clarify the contribution of the factor V Leiden mutation to recurrent miscarriages in these women.
  
  
  METHODS
  A prospective case control study between June 1999 and April 2000.
  
  
  METHODS
  Hospital University Science of Malaysia, Kubang Kerian, Kelantan, and Maternal and Child Health Clinic, Pasir Mas, Kelantan, Malaysia.
  
  
  METHODS
  A total of 46 Malay women with a history of three or more first or second trimester miscarriages were studied. The control group consisted of 46 parous women without obstetric complications.
  
  
  METHODS
  Diagnosis of factor V Leiden mutation was made by examination of factor V Leiden allele product following Mnl I digestion of factor V Leiden alleles amplified by polymerase chain reaction.
  
  
  RESULTS
  None of the 46 women with recurrent spontaneous abortion carried the mutation. Also, we found no subject carrying the factor V Leiden alleles in the control group.
  
  
  CONCLUSIONS
  These results suggest that that there is no association between the factor V Leiden mutation and recurrent spontaneous abortion in the Malay population.
authors.affiliation: Department of Haematology, School of Medical Sciences, University Science of Malaysia, Kelantan.
authors.forename: Narazah Mohd
authors.initials: NM
authors.lastname: Yusoff
authors.forename: Wan Zaidah
authors.initials: WZ
authors.lastname: Abdullah
authors.forename: Selamah
authors.initials: S
authors.lastname: Ghazali
authors.forename: Mohd Shukri
authors.initials: MS
authors.lastname: Othman
authors.forename: Abdul Aziz
authors.initials: AA
authors.lastname: Baba
authors.forename: Norazmi
authors.initials: N
authors.lastname: Abdullah
authors.forename: Mohd Nizam
authors.initials: MN
authors.lastname: Isa
authors.forename: Chan Li
authors.initials: CL
authors.lastname: Chong
mesh_terms: D000026:Abortion, Habitual / Q000453:epidemiology / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000483:Alleles; D001483:Base Sequence; D016022:Case-Control Studies; D005165:Factor V / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D015994:Incidence; D008296:Malaysia / Q000453:epidemiology; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D011446:Prospective Studies; D012008:Recurrence; D012016:Reference Values; D012307:Risk Factors
chemical_list: C095381:factor V Leiden; D005165:Factor V