Identification and characterization of foliar polyphenolic composition in sweetpotato (Ipomoea batatas L.) genotypes.
Trials over two years were conducted using 1389 sweetpotato (Ipomoea batatas L.) genotypes collected from all over the world to characterize the polyphenolic composition in sweetpotato leaves. Wide variation was observed in relation to their total and individual leaf polyphenolic constituents. In all genotypes studied, the total polyphenol contents of sweetpotato leaf ranged from 1.42 to 17.1 g/100 g dry weight. The six different polyphenolic compounds were identified and quantified by NMR, FABMS, and RPHPLC analysis procedures. This is the first report of polyphenolic compositions in sweetpotato leaves. The relative levels of polyphenolic acids in sweetpotato leaves were as follows: 3,5-di-O-caffeoylquinic acid > 4,5-di-O-caffeoylquinic acid > chlorogenic acid (3-O-caffeoylquinic acid) > 3,4-di-O-caffeoylquinic acid > 3,4,5-tri-O-caffeoylquinic acid > caffeic acid. The highest 3,4,5-tri-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid occurred at 221 and 1183.30 mg/100 g dry weight, respectively.
Islam
Md Shahidul
MS
Department of Upland Farming Research, National Agricultural Research Center for Kyushu Okinawa Region, Yokoichi 6651-2, Miyakonojo, Miyazaki 885-0091, Japan. mak825@affrc.go.jp
Yoshimoto
Makoto
M
Yahara
Shoji
S
Okuno
Shigenori
S
Ishiguro
Koji
K
Yamakawa
Osamu
O
D002109:Caffeic Acids / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids; D005838:Genotype; D027741:Ipomoea / Q000737:chemistry* / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D010636:Phenols / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry*; D011108:Polymers / Q000032:analysis*; D059808:Polyphenols; D013045:Species Specificity; D016339:Spectrometry, Mass, Fast Atom Bombardment
D002109:Caffeic Acids; D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D002726:Chlorogenic Acid; C040048:caffeic acid