title: Effects of recombinant staphylokinase on coronary thrombosis in Chinese experimental miniature swine.
abstract: OBJECTIVE
  To study the effects of recombinant staphylokinase (r-Sak) on coronary thrombosis, cardiac ischemia, and myocardial infarction in Chinese experimental miniature swine.
  
  
  METHODS
  Endarterium was injuried and coronary thrombi were formed gradually through direct electrical stimulation on the coronary artery of Chinese experimental miniature swine. Effects of r-Sak in vitro were studied through coronary pathological section, microkinematography, multi media graphic analysis, epicardial electrogram mapping, myocardium histo chemical stain, serum creatine phosphokinase (CPK), and hemorheology.
  
  
  RESULTS
  r-Sak showed a remarkable effect on coronary thrombus. Compared w ith the control group, the two dosage groups, r-Sak 0.45 mg/kg and 1.35 mg/kg, reduced the transverse section area of coronary thrombus (P<0.01), lessened the degree and range of cardiac ischemia (P<0.01), decreased the myocardial infarction area (P<0.01), the activity of CPK and blood viscosity (P<0.05), restrained platelet adhesion and aggregation, and reduced fibrinogen concentration (P<0.05).
  
  
  CONCLUSIONS
  r-Sak could dissolve coronary thrombus obviously and lessen the pathologic reaction of myocardium.
authors.affiliation: Department of Basic Medicine Science, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China. jianxun@ht.rol.cn.net
authors.forename: Jian-Xun
authors.initials: JX
authors.lastname: Liu
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Shang
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Fu
authors.forename: Xin-Zhi
authors.initials: XZ
authors.lastname: Li
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
mesh_terms: D000818:Animals; D003328:Coronary Thrombosis / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D003402:Creatine Kinase / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D018056:Hemorheology / Q000187:drug effects; D008297:Male; D008666:Metalloendopeptidases / Q000627:therapeutic use*; D009203:Myocardial Infarction / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000627:therapeutic use; D013556:Swine, Miniature
chemical_list: D005343:Fibrinolytic Agents; D011994:Recombinant Proteins; D003402:Creatine Kinase; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus