title: The effect of carbohydrate and fat variation in euenergetic diets on postabsorptive free fatty acid release.
abstract: Diet composition and energy content modulate free fatty acid (FFA) release. The aim of this study was to evaluate the dose-response effects of euenergetic variations in dietary carbohydrate and fat content on postabsorptive FFA release. The rate of appearance (Ra) of palmitate was measured by infusion of [2,2-2H2]palmitate after an overnight fast in six healthy men on three separate occasions, i.e. after 7 d on euenergetic control, high-carbohydrate and high-fat diets. The protein content and composition was identical for each diet. Postabsorptive plasma fatty acid concentrations were not different between the high-carbohydrate and control diets (0.36 (se 0.07) v. 0.43 (se 0.04) mmol/l), but were increased after the high-fat diet (0.75 (se 0.09) mmol/l, (P<0.01 compared with the other diets). Ra palmitate was not different between the high-carbohydrate and control diets (1.36 (se 0.20) v. 1.47 (se 0.15) micromol/kg per min). However, Ra palmitate was increased to 2.36 (se 0.26) micromol/kg per min after the high-fat diet (P<0.01 compared with the other diets). The fatty acid flux and whole-body fat oxidation were not affected by the high-carbohydrate diet compared with the control diet, but were increased by 67 and 47 % respectively, on the high-fat diet (P<0.01 compared with the other diets). A euenergetic high-fat diet results in increased postabsorptive FFA release and fat oxidation, whereas a euenergetic high-carbohydrate diet does not affect these variables of fat metabolism.
authors.affiliation: Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, The Netherlands. P.H.Bisschop@amc.uva.nl
authors.forename: Peter H
authors.initials: PH
authors.lastname: Bisschop
authors.forename: Mariëtte T
authors.initials: MT
authors.lastname: Ackermans
authors.forename: Erik
authors.initials: E
authors.lastname: Endert
authors.forename: An F C
authors.initials: AF
authors.lastname: Ruiter
authors.forename: Alfred J
authors.initials: AJ
authors.lastname: Meijer
authors.forename: Folkert
authors.initials: F
authors.lastname: Kuipers
authors.forename: Hans P
authors.initials: HP
authors.lastname: Sauerwein
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Romijn
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002096:C-Peptide / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000494:pharmacology*; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D019308:Palmitic Acid / Q000493:pharmacokinetics
chemical_list: D001786:Blood Glucose; D002096:C-Peptide; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D019308:Palmitic Acid