The preovulatory rise of ovarian ornithine decarboxylase is required for progesterone secretion by the corpus luteum.
Ovarian progesterone secretion during the diestrus stage of the estrous cycle is produced by luteal cells derived from granulosa and thecal cells after the differentiation process that follows ovulation. Our results show that blockade of the preovulatory rise of ovarian ornithine decarboxylase (ODC), a key enzyme in polyamine biosynthesis, by treatment with the specific inhibitor alpha-difluoromethylornithine (DFMO) leads to a significant decrease in the ovarian progesterone content and a dramatic fall in the plasma levels of this hormone during the following diestrus. The same inhibition was produced in spite of the fact that both luteinizing and follicle stimulating hormones were given concomitantly with DFMO. On the other hand, the acute rise in the plasma progesterone levels observed after administration of human chorionic gonadotropin to mice at different periods of the estrous cycle was not affected by DFMO administration. Our results indicate that although elevated levels of ODC are not required for acute ovarian steroidogenesis, the preovulatory peak of ovarian ODC activity observed in the evening of proestrus may be critical for the establishment of a constitutive steroidogenic pathway and progesterone secretion by the corpus luteum during the diestrus stage of the murine estrous cycle.
Bastida
Carmen M
CM
Department of Biochemistry, School of Medicine, University of Murcia, 30100 Murcia, Spain.
Tejada
Francisco
F
Cremades
Asunción
A
Peñafiel
Rafael
R
D000818:Animals; D003338:Corpus Luteum / Q000378:metabolism*; D000518:Eflornithine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004971:Estrus / Q000502:physiology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000494:pharmacology; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D065108:Ornithine Decarboxylase Inhibitors; D010053:Ovary / Q000201:enzymology*; D010060:Ovulation / Q000502:physiology; D011374:Progesterone / Q000378:metabolism*
D004791:Enzyme Inhibitors; D065108:Ornithine Decarboxylase Inhibitors; D011374:Progesterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D009955:Ornithine Decarboxylase; D000518:Eflornithine