A Vaccine to Coxsackievirus Prepared by High Pressure.
Effects of high hydrostatic pressure on infectious and immune activity of coxsackievirus group B (CVB) was described. CVB was completely inactivated at 230 MPa pressure and -16 degrees. The inactivated viruses remained highly immunogenic and induced CVB-specific serum antibody at a concentration as low as 1:1 500. 67% mice vaccinated with the pressurized viruses survived the challenge of fatal dose of CVB. These results indicated that the pressurized CVB induced immune reactions to protect mice from the viruses, and would be a potential vaccine candidate for human myocarditis.
Chen
Zhong-Ming
ZM
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China. kcruan@sunm.shcnc.ac.cn
Tian
Shao-Min
SM
Ruan
Kang-Cheng
KC