Cardiac gene expression profile and lipid accumulation in response to starvation.
Starvation induces many biochemical and histological changes in the heart; however, the molecular events underlying these changes have not been fully elucidated. To explore the molecular response of the heart to starvation, microarray analysis was performed together with biochemical and histological investigations. Serum free fatty acids increased twofold in both 16- and 48-h-fasted mice, and cardiac triglyceride content increased threefold and sixfold in 16- and 48-h-fasted mice, respectively. Electron microscopy showed numerous lipid droplets in hearts of 48-h-fasted mice, whereas fewer numbers of droplets were seen in hearts from 16-h-fasted mice. Expression of 11,000 cardiac genes was screened by microarrays. More than 50 and 150 known genes were detected by differential expression analysis after 16- and 48-h-fasts, respectively. Genes for fatty acid oxidation and gluconeogenesis were increased, and genes for glycolysis were decreased. Many other genes for metabolism, signaling/cell cycle, cytoskeleton, and tissue antigens were affected by fasting. These data provide a broad perspective of the molecular events occurring physiologically in the heart in response to starvation.
Suzuki
Jinya
J
Third Department of Internal Medicine, Fukui Medical University, Fukui 910-1193, Japan. jinya@fmsrsa.fukui-med.ac.jp
Shen
Wen-Jun
WJ
Nelson
Brett D
BD
Selwood
Simon P
SP
Murphy
Greer M
GM
Kanehara
Hideo
H
Takahashi
Sadao
S
Oida
Koji
K
Miyamori
Isamu
I
Kraemer
Fredric B
FB
Kanefara
Hideo
H
D000818:Animals; D015152:Blotting, Northern; D001835:Body Weight / Q000502:physiology; D002453:Cell Cycle / Q000235:genetics; D003599:Cytoskeleton / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005943:Gluconeogenesis / Q000235:genetics; D006019:Glycolysis / Q000235:genetics; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction; D015398:Signal Transduction / Q000235:genetics; D013217:Starvation / Q000378:metabolism*
D005227:Fatty Acids; D008055:Lipids