Translational regulation of human papillomavirus type 16 E7 mRNA by the peptide SEQIKA, shared by rabbit alpha(1)-globin and human cytokeratin 7.
The possible biochemical factors able to affect the in vitro expression of the high-risk human papillomavirus type 16 (HPV16) E7 oncoprotein have been analyzed. Evidence is provided that E7 mRNA stability is increased and, conversely, transcript translation is inhibited by binding to a 32-kDa protein from rabbit reticulocyte lysate; sequence analysis identified the 32-kDa binding protein as rabbit alpha(1)-globin protein; and interaction between rabbit alpha(1)-globin and E7 mRNA occurs through the 6-mer peptide SEQIKA present in human cytokeratin 7 protein. The in vitro data were confirmed by the occurrence of HPV16 E7 mRNA-cytokeratin 7 binding in squamous cervical cancer SiHa cells.
Kanduc
Darja
D
CARSO Cancer Research Center, Valenzano, Regione Puglia, Italy. d.kanduc@biologia.uniba.it
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D005914:Globins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D053552:Keratin-7; D007633:Keratins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D014407:Tumor Cells, Cultured
C507497:KRT7 protein, human; D053552:Keratin-7; C507496:Krt7 protein, mouse; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D010455:Peptides; D012333:RNA, Messenger; C059731:oncogene protein E7, Human papillomavirus type 16; D007633:Keratins; D005914:Globins