Mechanisms of metal ion action in Tn10 transposition.
Tn10/IS10 transposition involves assembly of a synaptic complex (or transpososome) in which two transposon ends are paired, followed by four distinct chemical steps at each transposon end. The chemical steps are dependent on the presence of a suitable divalent metal cation (Me(2+)). Transpososome assembly and structure are also affected by Me(2+). To gain further insight into the mechanisms of Me(2+) action in Tn10/IS10 transposition we have investigated the effects of substituting Mn(2+) for Mg(2+), the physiologic Me(2+), in transposition. We have also investigated the significance of an Me(2+)-assisted conformational change in transpososome structure. We show that Mn(2+) has two previously unrecognized effects on the Tn10 donor cleavage reaction. It accelerates the rates of hairpin formation and hairpin resolution without significantly affecting the rate of the first chemical step, first strand nicking. Mn(2+) also relaxes the specificity of first strand nicking. We also show that Me(2+)-assisted transpososome unfolding coincides with a structural transition in the transposon-donor junction that may be necessary for hairpin formation. Possible mechanisms for these observations are considered.
Allingham
John S
JS
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5B7.
Haniford
David B
DB
D020137:Base Pair Mismatch / Q000235:genetics; D001665:Binding Sites; D002384:Catalysis / Q000187:drug effects; D002413:Cations, Divalent / Q000494:pharmacology*; D003300:Copper / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018983:DNA Footprinting; D004251:DNA Transposable Elements / Q000235:genetics*; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D009154:Mutation; D009690:Nucleic Acid Conformation; D011995:Recombination, Genetic / Q000187:drug effects*; D019895:Transposases / Q000737:chemistry / Q000378:metabolism*
D002413:Cations, Divalent; D004251:DNA Transposable Elements; D008670:Metals; D008345:Manganese; D003300:Copper; D004247:DNA; D019895:Transposases