Non-covalent associations of cyclomaltooligosaccharides (cyclodextrins) with carotenoids in water. A study on the alpha- and beta-cyclodextrin/psi,psi-carotene (lycopene) systems by light scattering, ionspray ionization and tandem mass spectrometry.
Water-soluble complexes of the dietary carotenoid psi,psi-carotene (lycopene 1) with cyclomaltohexaose (alpha-cyclodextrin, alphaCD) and cyclomaltoheptaose (beta-cyclodextrin, betaCD) have been prepared and characterized via multiangle light scattering (MALS), ionspray/electrospray ionization (IS/ESI) mass spectrometry (MS) and tandem MS. MALS experiments point out that large aggregates of particles, on the nanometer-size scale, are present in water, with meaningful differences in the shape of the alphaCD/1 aggregates with respect to betaCD/1 analogues. The true 1:1 alphaCD/1 inclusion complex has been observed by IS/ESIMS and confirmed by tandem MS. The structure of CD/1 aggregations in water is proposed which are consistent with the combined MALS and MS experimental results.
Mele
Andrea
A
Dipartimento di Chimica, Materiali ed Ingegneria Chimica G. Natta del Politecnico di Milano, Via Mancinelli, 7, Italy.
Mendichi
Raniero
R
Selva
Antonio
A
Molnar
Peter
P
Toth
Gyula
G
D002338:Carotenoids / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D008027:Light; D000077276:Lycopene; D018613:Microscopy, Confocal; D015394:Molecular Structure; D012542:Scattering, Radiation; D021241:Spectrometry, Mass, Electrospray Ionization; D014867:Water / Q000737:chemistry
D003505:Cyclodextrins; D014867:Water; D002338:Carotenoids; D000077276:Lycopene