Role for complement receptors (CD21/CD35) in the regulation of recombination activating gene expression in murine peripheral B cells.
A population of peripheral B cells have been shown to express recombination activating gene products, RAG-1 and RAG-2, which are considered to be involved in revising the B cell antigen receptor (BCR) in the periphery. BCR engagement has been reported to turn off RAG expression in peripheral B cells, whereas the same treatment has an opposite effect on immature B cells in the bone marrow. In contrast to receptor editing that is involved in the removal of autoreactivity in immature B cells, it has been shown that secondary V(D)J rearrangement in peripheral B cells, termed receptor revision, contributes to affinity maturation of antibodies. Here, we show that RAG-2 expression in murine splenic B cells was abrogated by the coligation of BCR with complement receptors (CD21/CD35) much more efficiently than by the engagement of BCR alone. On the other hand, the same coligation augmented proliferation of anti-CD40-stimulated B cells. These findings suggest a crucial role for CD21/CD35 in directing the conservation or the revision of BCRs in peripheral B cells.
Ohmori
Hitoshi
H
Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Japan. hit2224@biotech.okayama-u.ac.jp
Magari
Masaki
M
Nakayama
Yasunori
Y
Kanayama
Naoki
N
Hikida
Masaki
M
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics*; D015536:Down-Regulation; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D017463:Receptors, Complement 3b / Q000378:metabolism*; D017464:Receptors, Complement 3d / Q000378:metabolism*; D011995:Recombination, Genetic; D013154:Spleen / Q000378:metabolism
D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d; C064655:V(D)J recombination activating protein 2