Release of NO by a nitrosyl complex upon activation by the mitochondrial reducing power.
The reaction of trans-[Ru(NH(3))(4)P(OEt)(3)NO](3+) and mitochondria was investigated through differential pulse polarography and fluorimetry. The nitrosyl complex undergoes one-electron reduction centered on the NO ligand site. The reaction between the mitochondrial reductor and trans-[Ru(NH(3))(4)P(OEt)(3)NO](3+) exhibits a second order specific rate constant calculated as k=2 x 10(1) M(-1) s(-1). The reduced species, trans-[Ru(NH(3))(4)P(OEt)(3)NO](2+), quickly releases NO, yielding trans-[Ru(NH(3))(4)P(OEt)(3)H(2)O](2+). The low toxicities of both trans-[Ru(NH(3))(4)P(OEt)(3)(NO)](2+) and trans-[Ru(NH(3))(4)P(OEt)(3)H(2)O](2+) and its ability to release NO after reductive activation in a biological medium make the nitrosyl compound a useful model of a hypotensive drug.
Toledo
José Carlos
JC
Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador São Carlense 400, CP 780, 13560-970, Brazil.
Lopes
Luiz Gonzaga de França
LG
Alves
Armindo Antonio
AA
Pereira da Silva
Lucia
L
Franco
Douglas Wagner
DW
D000818:Animals; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D009942:Organometallic Compounds / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D019163:Reducing Agents / Q000378:metabolism*; D017975:Ruthenium Compounds / Q000378:metabolism*
D009942:Organometallic Compounds; D019163:Reducing Agents; D017975:Ruthenium Compounds; D009243:NAD; D009569:Nitric Oxide