[Simulation of the migration process of human epidermal keratinocytes over three-dimensional collagen gel].
We developed a novel model for studying migration of keratinocyte colonies over 3D collagen gel. It was shown that keratinocytes previously cultured on plastic and then seeded on the surface of collagen gel proliferate more actively than the primary cells. The gel density could significantly affect the morphology and migration of keratinocyte colonies. Modification of collagen gel by fibronectin-like protein or poly-L-lysine altered the morphometric parameters of colonies without significant changes in the velocity of migration.
Voroteliak
E A
EA
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 117808 Russia.
Shikhverdieva
A Sh
ASh
Vasil'ev
A V
AV
Terskikh
V V
VV
D018929:Cell Culture Techniques / Q000379:methods*; D002465:Cell Movement; D002478:Cells, Cultured; D003094:Collagen; D005353:Fibronectins / Q000737:chemistry; D005782:Gels; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D010455:Peptides / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry
D005353:Fibronectins; D005782:Gels; D010455:Peptides; D011107:Polylysine; D003094:Collagen
Modulirovanie protsessa migratsii épidermal'nykh keratinotsitov cheloveka po trekhmernomy kollagenovomu geliu.