title: Intracellular localization of the particulate methane monooxygenase and methanol dehydrogenase in Methylomicrobium album BG8.
abstract: The methanotrophic bacterium Methylomicrobium album BG8 uses methane as a sole source of carbon and energy. This bacterium forms an extensive intracytoplasmic membrane. The first enzymes of the methane oxidation pathway are the membrane-bound particulate methane monooxygenase and the periplasmic methanol dehydrogenase. Immunoelectron microscopy with specific antibodies was used to localize these enzymes to the intracytoplasmic membrane.
authors.affiliation: Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, WI 53201, USA.
authors.forename: Christine A
authors.initials: CA
authors.lastname: Brantner
authors.forename: Charles C
authors.initials: CC
authors.lastname: Remsen
authors.forename: Heather A
authors.initials: HA
authors.lastname: Owen
authors.forename: Lorie A
authors.initials: LA
authors.lastname: Buchholz
authors.forename: Mary Lynne
authors.initials: ML
authors.lastname: Perille Collins
mesh_terms: D000429:Alcohol Oxidoreductases / Q000032:analysis*; D000918:Antibody Specificity; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000201:enzymology / Q000648:ultrastructure; D008565:Membrane Proteins; D008772:Methylococcaceae / Q000201:enzymology*; D016253:Microscopy, Immunoelectron / Q000379:methods; D010105:Oxygenases / Q000032:analysis* / Q000737:chemistry
chemical_list: D008565:Membrane Proteins; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); D010105:Oxygenases; C021555:methane monooxygenase