The value of microarray techniques for quantitative gene profiling in molecular diagnostics.
There has been an explosion of interest in microarray technologies that allow the quantification of whole-genome RNA expression data. The apparent correlation of expression profiles with clinically relevant parameters such as disease outcome has raised expectations with respect to the clinical usefulness of the data generated. Yet the accuracy and biological relevance of these data remain contentious, even in basic research applications. Therefore, numerous issues related to format, quality, validation and interpretation remain to be resolved before microarray profiling can become a diagnostic tool of clinical relevance for routine work.
Bustin
Stephen A
SA
Academic Dept of Surgery, Barts, London Queen Mary's School of Medicine and Dentistry, University of London, London, UK, E1 1BB. s.a.bustin@qmul.ac.uk
Dorudi
Sina
S
D020869:Gene Expression Profiling / Q000379:methods / Q000592:standards; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods* / Q000592:standards; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000592:standards* / Q000639:trends; D009928:Organ Specificity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity