Using expressed sequence tag databases to identify ovarian genes of interest.
GenBank contains 4879 expressed sequence tags (EST) derived from four non-normalized human ovarian cDNA libraries. Of these EST, 2646 are contributors to UniGene clusters and have UniGene numbers. The EST map to 1206 distinct UniGenes. A gene expression profile was established for the human ovary by identifying the abundance of each UniGene cluster and its corresponding annotation. The most highly expressed transcripts were for proteins associated with protein synthesis (ribosomal proteins, elongation factors, thymosins, etc.). However, there are also transcripts for genes of unknown function that are ovary-specific. This ovarian gene expression profile provides useful data for the design of DNA microarrays targeted at ovarian function and highlights novel sequences that warrant further investigation.
Stanton
J L
JL
Department of Anatomy and Structural Biology, University of Otago Medical School, P.O. Box 913, Dunedin, New Zealand.
Macgregor
A B
AB
Green
D P L
DP
D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010053:Ovary / Q000502:physiology*