title: Stress-induced changes of plasma antioxidants in aquacultured sea bass, Dicentrarchus labrax.
abstract: Antioxidant plasma activities of ascorbate, alpha-tocopherol and glutathione peroxidase were analysed in adult male sea bass, Dicentrarchus labrax, in normal conditions and after hypoxia-recovery. In addition, tank measurements of temperature, pH, salinity and chlorine changes were carried out. Ascorbate and alpha-tocopherol were measured using a high-pressure liquid chromatography method and glutathione peroxidase activity enzymatically. Ascorbate and alpha-tocopherol showed a relationship with the velocity of body growing in normal and hypoxia-recovery conditions. In sea bass exposed to hypoxia, only ascorbate and alpha-tocopherol levels were significantly lower compared with the control group. Slope study and expression percent of antioxidants reduction after stress conditions revealed a predominant role of plasma alpha-tocopherol. Sea bass subjected to variations of salinity and chlorine showed a significant decrease in plasma alpha-tocopherol. A relationship could be suggested between antioxidant defence and fish response in aquaculture.
authors.affiliation: Department of Zoology, Federico II University of Naples, Via Mezzocannone 8, 80134 Naples, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Guerriero
authors.forename: Alessandra
authors.initials: A
authors.lastname: Di Finizio
authors.forename: Gaetano
authors.initials: G
authors.lastname: Ciarcia
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D017756:Aquaculture; D001205:Ascorbic Acid / Q000097:blood*; D001492:Bass / Q000097:blood*; D001835:Body Weight; D002713:Chlorine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005979:Glutathione Peroxidase / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000097:blood; D008297:Male; D009994:Osmolar Concentration; D012623:Seawater / Q000737:chemistry; D013312:Stress, Physiological / Q000097:blood* / Q000503:physiopathology*; D013696:Temperature; D024502:alpha-Tocopherol / Q000097:blood*
chemical_list: D000975:Antioxidants; D002713:Chlorine; D005979:Glutathione Peroxidase; D024502:alpha-Tocopherol; D001205:Ascorbic Acid