Effect of pre-adsorbed proteins on attachment, proliferation, and function of endothelial cells.
As certain proteins control cell adhesion, it has been hoped that cell transplantation and tissue engineering could be augmented by pre-adsorption of specific proteins to biological or synthetic surfaces. The questions that remain, however, are whether such proteins can affect cell production as well as adhesion, and if so, whether in a protein-specific manner. We examined the adhesion and the biochemical secretion of bovine aortic endothelial cells (BAEC) on tissue culture polystyrene (TCPS) discs coated with fibronectin (Fn), laminin (Ln), or gelatin. The three coating proteins nonspecifically promote sub-confluent and post-confluent endothelial cell production of total protein up to 2.5-fold of the reference value. Total soluble glycosaminoglycan (GAG) production slightly increased with the different coatings only at low cell density. In contrast, Ln and Fn, not gelatin, drastically enhanced post-confluent BAEC production of prostaglandin (PGI2). However, antibody-blockage of the alpha5 integrin, constituent of the Fn receptor in BAEC, appeared to inhibit the upregulation of PGI2 production observed on Fn-coated surfaces. The results indicate that the cell adhesion mediators used as coating agents dictate cell biological production as well as adhesion and proliferation.
Balcells
Mercedes
M
Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge 02139, USA. merche@mit.edu
Edelman
Elazer R
ER
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D002455:Cell Division / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism*; D011464:Epoprostenol / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology*; D005780:Gelatin / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000378:metabolism; D007700:Kinetics; D007797:Laminin / Q000494:pharmacology*; D017469:Receptors, Fibronectin / Q000037:antagonists & inhibitors / Q000378:metabolism; D016378:Tissue Transplantation / Q000379:methods
D005353:Fibronectins; D006025:Glycosaminoglycans; D007797:Laminin; D017469:Receptors, Fibronectin; D005780:Gelatin; D011464:Epoprostenol