[Production of regenerants in sugar beet].
The experimental results are presented on production of plants-regenerants of the sugar beet (Beta vulgaris L.) via callus formation or direct organogenesis from the leaf tissues. The method of aseptic treatment was developed for the seeds with strong bacterial and fungal invasion. The regenerant plants were obtained in the presence of various concentrations of synthetic hormones, such as cytokinin (6-benzaminopurine) and auxin (naphthylacetic acid), and inhibitor of auxin transport in plants (2,3,5-triiodobenzoic acid). This combination of growth regulators made it possible to avoid callus formation. The genotype of initial plants affected the capacity for callus formation and regeneration. The temperature influenced rhizogenesis in regenerants.
Kolodiazhnaia
Ia S
IaS
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrent'eva 10, Novosibirsk, 630090 Russia.
Deĭneko
E V
EV
D000085:Acetates / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001593:Benzyl Compounds; D027461:Beta vulgaris / Q000187:drug effects / Q000382:microbiology / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D002678:Chimera; D003470:Culture Media; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin; D009281:Naphthalenes / Q000494:pharmacology; D011687:Purines; D012038:Regeneration; D012230:Rhizobiaceae; D012639:Seeds / Q000502:physiology; D014283:Triiodobenzoic Acids / Q000494:pharmacology
D000085:Acetates; D001593:Benzyl Compounds; D003470:Culture Media; D007210:Indoleacetic Acids; D009281:Naphthalenes; D011687:Purines; D014283:Triiodobenzoic Acids; C052968:2,3,5-triiodobenzoic acid; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin
Poluchenie regenerantov u sakharnoĭ svekly.