[Effect of diamide on protein oxidation and physico-chemical properties of lipids in erythrocyte membranes].
The effect of diamide on the physicochemical state of proteins and lipids of human erythrocyte membrane was studied. It was found that diamide at a concentration of 1 mM decreases the content of the SH-groups of membrane proteins by approximately 50%, resulting in enhanced vesiculation of erythrocytes upon metabolic exhaustion of cells. It was shown using fluorescein isothiocyanate-labeled concanavalin A and 4,4'-diisothiocyano-2,2'-stilbene disulfonate that diamide changes the structural state of the main integral protein of erythrocyte membranes, the band 3 protein. Changes in the microviscosity of the membrane lipid bilayer depending on diamide concentration were determined from the changes in the fluorescence parameters of the lipophilic probes (pyrene and 1,6-diphenyl-3,5-hexatriene). The level of lipid peroxidation products in membranes remained unchanged. It follows from these data that the SH-oxidizing agent diamide does not directly interact with the lipid bilayer of membrane and produces changes in the physicochemical state of lipids presumably by disrupting protein-lipid interactions that take place upon oxidation of the SH-groups and cross-linking of membrane proteins.
Kozlova
N M
NM
Institute of Photobiology, National Academy of Sciences of Belarus, Academicheskaya ul. 27, Minsk, 220072 Belarus.
Luk'ianenko
L M
LM
Antonovich
A N
AN
Kut'ko
A G
AG
Zubritskaia
G P
GP
Slobozhanina
E I
EI
D003958:Diamide / Q000494:pharmacology*; D004161:Diphenylhexatriene; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D005456:Fluorescent Dyes; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011506:Proteins / Q000378:metabolism*; D011721:Pyrenes; D013439:Sulfhydryl Reagents / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D005456:Fluorescent Dyes; D008563:Membrane Lipids; D011506:Proteins; D011721:Pyrenes; D013439:Sulfhydryl Reagents; D017392:Thiobarbituric Acid Reactive Substances; D003958:Diamide; D004161:Diphenylhexatriene; C030984:pyrene
Okislitel'naia modifikatsiia belkov i fiziko-khimicheskoe sostoianie lipidov v membranakh éritrotsitov pri deĭstvii diamina.