Crystallization and preliminary crystallographic analysis of manganese superoxide dismutase from Bacillus halodenitrificans.
Manganese superoxide dismutase (GP-MnSOD), a component of the so-called 'green protein' (green protein complex) from the facultative anaerobic halodenitrifier Bacillus halodenitrificans, has been crystallized using the hanging-drop vapor diffusion method. Crystals have unit-cell parameters a=b=93.4 A, c=65.0 A, and belong to the space group P4(3)2(1)2. Preliminary analysis indicates there is one monomer in each asymmetric unit. The structural information from this enzyme will enrich our knowledge on its high catalytic activity and its possible role in green protein complex.
Liao
Jun
J
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.
Li
Mei
M
Liu
Ming-Yih
MY
Chang
Tschining
T
Le Gall
Jean
J
Gui
Lu-Lu
LL
Zhang
Ji-Ping
JP
Liang
Dong-Cai
DC
Chang
Wen-Rui
WR
D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D013482:Superoxide Dismutase / Q000737:chemistry*
D013482:Superoxide Dismutase