title: Comparative mapping of five coding DNA sequences on cattle chromosomes 7 and 25.
abstract: Comparative mapping of four genes and one unknown coding DNA sequence in breakpoint positions of bovine chromosomes (BTA) 7 and 25 are presented. Performing a genome data base search five bovine expressed sequence tags from the MARC library matched with human genes coding for the general transcription factor IIIC polypeptide 1 (GTF3C1), the hypothetical protein KIAA0556, the interleukin 4 receptor (IL4R), the regulatory factor X-associated ankyrin-containing protein (RFXANK), and with an unknown human coding sequence partially homologous to the genomic cosmid clone R30923. Loci for these sequences were COMPASS predicted on BTA7 or BTA18 and to BTA18 or BTA25. Mapping was performed in a cattle-hamster somatic hybrid cell panel and a cattle-hamster 5000 rad whole genome radiation hybrid panel. GTF3C1, KIAA0556 and IL4R were assigned to the centromere region of BTA25 and RFXANK and R30923 close to the centromere of BTA7. The assignments contribute to the identification of evolutionary chromosome break points between human chromosomes 16 and 19 and BTA7, BTA18, and BTA25.
authors.affiliation: Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, USA. goldamme@gmx.de
authors.forename: T
authors.initials: T
authors.lastname: Goldammer
authors.forename: S R
authors.initials: SR
authors.lastname: Kata
authors.forename: R M
authors.initials: RM
authors.lastname: Brunner
authors.forename: M
authors.initials: M
authors.lastname: Schwerin
authors.forename: J E
authors.initials: JE
authors.lastname: Womack
mesh_terms: D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D002875:Chromosomes; D003360:Cosmids; D006224:Cricetinae; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D019948:Receptors, Interleukin-4 / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D019474:Transcription Factors, TFIII / Q000235:genetics*; D000098832:Transcription Factor TFIIIC
chemical_list: D004268:DNA-Binding Proteins; D005819:Genetic Markers; C117892:RFXANK protein, human; D019948:Receptors, Interleukin-4; D014157:Transcription Factors; D019474:Transcription Factors, TFIII; D000098832:Transcription Factor TFIIIC