Determination of acaricides in honey by high-performance liquid chromatography with photodiode array detection.
Rapid analytical methods are described to control quality of honeys, concerning residues of acaricides applied in hives to prevent Varroa jacobsoni infestation. A liquid-liquid extraction with hexane-propanol-2-ammonia (60 ml:30 ml:0.28%) was used for the simultaneous analysis of coumaphos, bromopropylate, amitraz and fluvalinate. For thymol, one clean up on a solid-phase extraction C18 (500 mg, 6 ml) column was performed; for rotenone, a liquid extraction with dichloromethane was realised. Quantitative recoveries obtained with honey were satisfactory and were superior to 80%. All acaricides are identified by reversed-phase high-performance liquid chromatography with photodiode array detection. Quantification limits obtained were below maximal residue limits when these exist.
Martel
Anne-Claire
AC
Agence Française de Sécurité Sanitaire des Aliments (AFSSA) Site de Sophia Antipolis, Laboratoire d'Etudes et de Recherches sur les Petits Ruminants et les Abeilles, France. ac.martel@sophia.afssa.fr
Zeggane
Sarah
S
D000053:Acari; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006722:Honey / Q000032:analysis*; D007306:Insecticides / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*
D007306:Insecticides; D010573:Pesticide Residues