title: Molecular cloning of a vaccine antigen against infection with the larval stage of Echinococcus multilocularis.
abstract: Alveolar and cystic hydatidosis are caused by infection with the larval stages of Echinococcus multilocularis and Echinococcus granulosus, respectively. A host-protective antigen has been identified in E. granulosus. Here we identify the presence of a closely related protein in E. multilocularis, characterize and express a cDNA encoding the antigen (designated EM95), determine the structure of the em95 gene, and demonstrate that the EM95 recombinant protein can be used to induce significant levels of protection against challenge infection with E. multilocularis eggs in mice.
authors.affiliation: Molecular Parasitology Laboratory, Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia. charlesg@unimelb.edu.au
authors.forename: Charles
authors.initials: C
authors.lastname: Gauci
authors.forename: Michael
authors.initials: M
authors.lastname: Merli
authors.forename: Volker
authors.initials: V
authors.lastname: Muller
authors.forename: Conan
authors.initials: C
authors.lastname: Chow
authors.forename: Kinpei
authors.initials: K
authors.lastname: Yagi
authors.forename: Ute
authors.initials: U
authors.lastname: Mackenstedt
authors.forename: Marshall W
authors.initials: MW
authors.lastname: Lightowlers
mesh_terms: D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D003411:Arvicolinae; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004195:Disease Models, Animal; D004443:Echinococcosis / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D004445:Echinococcosis, Pulmonary / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D004446:Echinococcus / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D005260:Female; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology; D007814:Larva; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D020442:Poloxamer; D011136:Polysorbates; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D016205:Sigmodontinae; D013185:Squalene / Q000031:analogs & derivatives*; D014612:Vaccines; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000947:Antigens, Helminth; D018076:DNA, Complementary; C446034:EG95 protein, Echinococcus granulosus; C460870:EM95 protein, Echinococcus multilocularis; D015801:Helminth Proteins; D011136:Polysorbates; D011993:Recombinant Fusion Proteins; D014612:Vaccines; D014614:Vaccines, Synthetic; D020442:Poloxamer; D013185:Squalene; C019556:squalane