title: Growth suppression and immunogenicity enhancement of Hep-2 or primary laryngeal cancer cells by adenovirus-mediated co-transfer of human wild-type p53, granulocyte-macrophage colony-stimulating factor and B7-1 genes.
abstract: Co-transfer of immunomodulatory and antiproliferative genes may be the basis for new strategies to potentiate tumor regression. In this study, we evaluated the in vitro effect of the introduction of human wild-type p53, granulocyte-macrophage colony-stimulating factor (GM-CSF), and B7-1 genes via recombinant adenovirus on the growth and immunogenicity of Hep-2 or primary laryngeal cancer cells. By the introduction of wild-type p53 gene, the growth of Hep-2 cells was inhibited via enhanced apoptosis. By the introduction of GM-CSF and B7-1 genes, the immunogenicity of cancer cells was enhanced. Significant proliferation of tumor infiltrating lymphocytes (TILs) and tumor-specific cytotoxicity of cytotoxic T lymphocytes (CTLs) were induced in vitro. Furthermore, the combinative effect of GM-CSF and B7-1 was even more evident than that of any one of them singly. These results suggest that the co-transfer of human wild-type p53, GM-CSF and B7-1 genes into tumor cells via recombinant adenovirus may be further developed into a potential combination gene therapy strategy for cancer.
authors.affiliation: Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, P.R. China.
authors.forename: Zhao-hua
authors.initials: ZH
authors.lastname: Qiu
authors.forename: Chu-tse
authors.initials: CT
authors.lastname: Wu
authors.forename: Miao-fen
authors.initials: MF
authors.lastname: Lao
authors.forename: Lu-zhe
authors.initials: LZ
authors.lastname: Pan
authors.forename: Yuan-min
authors.initials: YM
authors.lastname: Li
mesh_terms: D000256:Adenoviridae; D017209:Apoptosis; D018122:B7-1 Antigen / Q000235:genetics*; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D016158:Genes, p53; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics*; D049452:Green Fluorescent Proteins; D006801:Humans; D007668:Kidney; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000473:pathology*; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000032:analysis; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D018122:B7-1 Antigen; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor