Preferential incorporation of trans, trans-conjugated linoleic acid isomers into the liver of suckling rats.
The present study was designed to compare the conjugated linoleic acid (CLA) isomeric distribution pattern in the liver of suckling rats in relation to those in the milk and maternal diet. Silver-ion HPLC was used to separate individual CLA isomers. It was found that the isomeric distribution pattern in the milk was very similar to that in the maternal dietary fat. However, the CLA isomeric distribution patterns in the liver phospholipids (PL) and triacylglycerols were different from those in the diet and milk. In the liver PL, total cisltrans isomers accounted for 63.6-63.9% of total CLA, which was in contrast to the values of 88.1-89.1% in the milk and diet. In the liver PL, total transltrans isomers were 20.6-20.8% of the total CLA isomers whereas they were only 2.6-3.7% in the milk and diet. It is concluded that trans/trans-CLA were preferentially incorporated into the liver whereas for the incorporation of cis/trans-CLA there was partial discrimination.
Yang
Lin
L
Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, The People's Republic of China.
Yeung
Sai Ying Venus
SY
Huang
Yu
Y
Wang
Han Qing
HQ
Chen
Zhen-Yu
ZY
D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004041:Dietary Fats / Q000493:pharmacokinetics; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D007536:Isomerism; D007774:Lactation / Q000502:physiology*; D008041:Linoleic Acids / Q000493:pharmacokinetics*; D008099:Liver / Q000033:anatomy & histology / Q000378:metabolism*; D008892:Milk / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000033:anatomy & histology
D004041:Dietary Fats; D005227:Fatty Acids; D008041:Linoleic Acids; D010743:Phospholipids