title: Plasma growth hormone and growth hormone-binding protein during development in the marsupial brushtail possum (Trichosurus vulpecula).
abstract: Plasma concentrations of growth hormone (GH) were measured in the brushtail possum (Trichosurus vulpecula) pouch young from 25 through to 198 days post-partum (n=71). GH concentrations were highest early in pouch life (around 100 ng/ml), and thereafter declined in an exponential fashion to reach adult concentrations (10.8+/-1.8 ng/ml; n=21) by approximately 121-145 days post-partum, one to two months before the young is weaned. Growth hormone-binding protein (GHBP), which has been shown to modify the cellular actions of GH in eutherian mammals, was identified for the first time in a marsupial. Based on size exclusion gel filtration, possum GHBP had an estimated molecular mass of approximately 65 kDa, similar to that identified in other mammalian species, and binding of (125)I-labelled human GH (hGH) was displaced by excess hGH (20 microg). An immunoprecipitation method, in which plasma GHBP was rendered polyethylene glycol precipitable with a monoclonal antibody to the rabbit GHBP/GH receptor (MAb 43) and labelled with (125)I-hGH, was used to quantitate plasma GHBP by Scatchard analysis in the developing (pooled plasma samples) and adult (individual animals) possums. Binding affinity (K(a)) values in pouch young aged between 45 and 54 and 144 and 153 days post-partum varied between 1.0 and 2.4 x 10(9)/M, which was slightly higher than that in adult plasma (0.96+/-0.2 x 10(9)/M, n=6). Binding capacity (B(max)) values increased from non-detectable levels in animals aged 25-38 days post-partum to reach concentrations around half that seen in the adult (1.4+/-0.2 x 10(-9) M) by about 117 days post-partum and remained at this level until 153 days post-partum. Therefore, in early pouch life when plasma GH concentrations are highest, the very low concentrations of GHBP are unlikely to be important in terms of competing with GH-receptor for ligand or altering the half-life of circulating GH.
authors.affiliation: School of Biomedical Sciences, Department of Physiology and Pharmacology, The University of Queensland, Queensland 4072, Australia.
authors.forename: M C
authors.initials: MC
authors.lastname: Saunders
authors.forename: R T
authors.initials: RT
authors.lastname: Gemmell
authors.forename: M J
authors.initials: MJ
authors.lastname: Waters
authors.forename: J D
authors.initials: JD
authors.lastname: Curlewis
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000097:blood*; D002850:Chromatography, Gel; D005260:Female; D013006:Growth Hormone / Q000097:blood*; D006207:Half-Life; D008297:Male; D008892:Milk / Q000737:chemistry; D008970:Molecular Weight; D009893:Opossums / Q000097:blood* / Q000254:growth & development*; D011233:Precipitin Tests; D011247:Pregnancy; D011485:Protein Binding
chemical_list: D002352:Carrier Proteins; D013006:Growth Hormone; C051815:somatotropin-binding protein