Efficient embedding technique for preparing small specimens for stereological volume estimation: zebrafish larvae.
Stereological sampling regimes, in particular volume and number estimation, often require systematic uniformly random sections throughout a specimen. A method has been developed to increase the efficiency of preparing fish larvae for sectioning prior to histological or stereological analysis. Embedding a group of larvae in a resin block using this technique greatly reduces the quantity of sections produced and allows easy assessment of sample groups. Saving time in this way therefore makes stereology a more viable research tool.
Hill
A J
AJ
Developmental Toxico-Pathology Research Group, Department of Human Anatomy and Cell Biology, Faculty of Medicine, Ashton Street, University of Liverpool, Liverpool L69 3GE, UK. a.j.hill@liv.ac.uk
Howard
C V
CV
Cossins
A R
AR
D000818:Animals; D006652:Histological Techniques; D007814:Larva / Q000033:anatomy & histology; D016613:Plastic Embedding / Q000295:instrumentation / Q000379:methods*; D013048:Specimen Handling; D016610:Tissue Embedding / Q000379:methods; D015027:Zebrafish / Q000033:anatomy & histology*