Detection of CMV in plasma and aqueous humor specimens from AIDS patients with CMV retinitis using the amplicor CMV test.
A DNA hybridization test (the Amplicor CMV test) for CMV DNA was used to detect CMV DNA in paired plasma and aqueous humor specimens from 37 AIDS patients with ophthalmoscopically diagnosed CMV retinitis. CMV was detected in 25/37 (67.6%) plasma specimens and in 32/37 (86.5%) aqueous humor specimens. Thus, the Amplicor CMV test using aqueous humor specimens seems to be a useful diagnostic test for CMV retinitis and one that is more sensitive than determination of CMV DNA in plasma specimens.
Yamamoto
Narumichi
N
Department of Ophthalmology, University of Tsukuba, Ibaraki, Japan.
Wakabayashi
Taketoshi
T
Murakami
Kimio
K
Hommura
Sachiko
S
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000821:virology*; D001082:Aqueous Humor / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D017726:Cytomegalovirus Retinitis / Q000175:diagnosis / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis / Q000097:blood*; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic