Role of the second immunoglobulin-like loop of nectin in cell-cell adhesion.
Nectin is a Ca(2+)-independent immunoglobulin (Ig)-like cell-cell adhesion molecule that forms cell-cell adherens junctions cooperatively with E-cadherin in a variety of cells. Nectin has one transmembrane segment and three Ig-like loops in the extracellular region. The first Ig-like loop is essential for the trans-dimer formation of nectin of two neighboring cells, causing cell-cell adhesion. We show here that the second Ig-like loop is essential for the cis-dimer formation of nectin on the same cell, and that the cis-dimer formation is essential for the trans-dimer formation.
Momose
Yumiko
Y
Department of Molecular Biology and Biochemistry, Faculty of Medicine, Osaka University Graduate School of Medicine, Suita 565-0871, Japan.
Honda
Tomoyuki
T
Inagaki
Maiko
M
Shimizu
Kazuya
K
Irie
Kenji
K
Nakanishi
Hiroyuki
H
Takai
Yoshimi
Y
D000818:Animals; D019556:COS Cells; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000494:pharmacology / Q000502:physiology*; D002449:Cell Aggregation; D002522:Chlorocebus aethiops; D019281:Dimerization; D007136:Immunoglobulins / Q000494:pharmacology / Q000502:physiology; D051379:Mice; D000075983:Nectins; D017433:Protein Structure, Secondary; D014162:Transfection
D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D000075983:Nectins