Mixed patterns of transmission of human herpesvirus-8 (Kaposi's sarcoma-associated herpesvirus) in Malawian families.
To study transmission patterns of human herpesvirus-8 (HHV-8) (Kaposi's sarcoma-associated herpesvirus) in families in Malawi, nucleotide sequences derived from two hypervariable loci of the HHV-8 genome, the V1 and V2 regions of open reading frame K1 (K1/V1 and K1/V2, respectively), were amplified from blood and mouth rinse samples of 22 patients with treated and untreated Kaposi's sarcoma (KS) and their first-degree relatives (n=67). In patients with KS, vincristine therapy was significantly associated with non-detectability of circulating, but not oral, K1/V1 DNA. Intra-familial K1/V1 phylogenetic comparisons of eight families were possible. Both identical and non-identical sequences were observed between family members, suggesting transmission of HHV-8 along both intra- and extra-familial transmission routes.
Cook
Rachelle D
RD
Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK2.|Department of Oral Medicine, Eastman Dental Institute for Oral Health Care Sciences, UCL, University of London, UK1.
Hodgson
Tim A
TA
Department of Oral Medicine, Eastman Dental Institute for Oral Health Care Sciences, UCL, University of London, UK1.
Waugh
Alastair C W
ACW
Department of Oral Medicine, Eastman Dental Institute for Oral Health Care Sciences, UCL, University of London, UK1.
Molyneux
Elizabeth M
EM
Department of Paediatrics3 and Department of Surgery4, College of Medicine, University of Malawi, Blantyre, Malawi.
Borgstein
Eric
E
Department of Paediatrics3 and Department of Surgery4, College of Medicine, University of Malawi, Blantyre, Malawi.
Sherry
A
A
Department of Paediatrics3 and Department of Surgery4, College of Medicine, University of Malawi, Blantyre, Malawi.
Teo
Chong Gee
CG
Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK2.
Porter
Stephen R
SR
Department of Oral Medicine, Eastman Dental Institute for Oral Health Care Sciences, UCL, University of London, UK1.
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004279:DNA, Viral / Q000235:genetics; D018562:Disease Transmission, Infectious; D005260:Female; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006801:Humans; D007223:Infant; D008295:Malawi / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012514:Sarcoma, Kaposi / Q000188:drug therapy / Q000453:epidemiology*; D016415:Sequence Alignment; D014750:Vincristine / Q000627:therapeutic use
D000972:Antineoplastic Agents, Phytogenic; D004279:DNA, Viral; D014750:Vincristine