title: Production and characterization of a thermostable glucoamylase from Streptosporangium sp. endophyte of maize leaves.
abstract: Thermostable amylolytic enzymes are currently investigated to improve industrial processes of starch degradation. Streptosporangium sp. an endophytic actinomycete isolated from leaves of maize (Zea mays L.) showed glucoamylase production, using starch-Czapek medium, and the highest rate was obtained in the initial growth phase, after incubation for 24 h at pH 8.0. Maximum glucoamylase activity (158 U mg(-1) protein) was obtained at pH 4.5 and 70 degrees C. The isolated enzyme exhibited thermostable properties as indicated by retention of 100% of residual activity at 70 degrees C for 30 min with total inhibition at 100 degrees C. Extracellular enzyme from Streptosporangium sp. was purified by fractionated precipitation with ammonium sulphate. After 60% saturation produced 421 U mg(-1) protein, and yield was 74% with purification 2.7 fold. The enzyme produced by Streptosporangium sp. has potential for industrial applications.
authors.affiliation: Department of Nutrition, Institute of Antibiotics, University Federal of Pernambuco, Recife, Brazil. newtonps@novaera.com.br
authors.forename: T L M
authors.initials: TL
authors.lastname: Stamford
authors.forename: N P
authors.initials: NP
authors.lastname: Stamford
authors.forename: L C B B
authors.initials: LC
authors.lastname: Coelho
authors.forename: J M
authors.initials: JM
authors.lastname: Araújo
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D000645:Ammonium Sulfate / Q000494:pharmacology; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D013997:Time Factors; D003313:Zea mays / Q000382:microbiology*
chemical_list: D005087:Glucan 1,4-alpha-Glucosidase; D000645:Ammonium Sulfate