Group B streptococcus (GBS) modifies macrophage phosphatidylserine metabolism during induction of apoptosis.
Group B streptococcus (GBS) induced macrophage apoptosis by which it could avoid host defence mechanisms. Macrophages, which constitutively express phosphatidylserine (PtdSer) on the outer leaflet of plasma membrane, increased PtdSer exposure during GBS-induced apoptosis. Induction of apoptosis decreased PtdSer radioactivity of macrophages incubated with [(3)H]serine. The effect appeared not due to increasing conversion of PtdSer to phosphatidylethanolamine or phosphatidylcholine nor to the release of radioactive membrane vesicles. The radioactivity in lysoPtdSer was also reduced. These results confirm that induction of apoptosis involves a modification of PtdSer metabolism and point out the typical features of the GBS-induced apoptosis with respect to other models of apoptosis.
Buratta
Sandra
S
Department of Internal Medicine, Biochemistry Section, University of Perugia, 06122 Perugia, Italy.
Fettucciari
Katia
K
Mambrini
Raffaela
R
Fetriconi
Ilaria
I
Marconi
Pierfrancesco
P
Mozzi
Rita
R
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology; D008297:Male; D051379:Mice; D019884:Nitrogenous Group Transferases / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism*; D013292:Streptococcus agalactiae / Q000254:growth & development / Q000502:physiology*; D013997:Time Factors
D010718:Phosphatidylserines; D019884:Nitrogenous Group Transferases; C022370:phospholipid serine base exchange enzyme