Prime-boost: the way forward for recombinant vaccines against apicomplexan parasites. A Theileria perspective.
A good CD8+ response is required for immunity to many intracellular pathogens. Traditional antigen delivery using isolated sub-units or killed pathogens and adjuvant stimulates strong antibody responses but weak T-cell reactivity. Attenuated vaccines are usually more effective. The prime-boost delivery system based on immunisation with a naked DNA antigen-gene construct followed by boosting with an attenuated vaccinia virus expressing the same antigen is proving to be a powerful way to stimulate antigen-specific CD8 responses. Success using single epitopes is possible in malaria and we believe this approach holds much promise for other apicomplexan parasites such as Theileria spp.
Hall
R
R
Department of Biology, University of York, UK. frh1@york.ac.uk
Adamson
R
R
Boulter
N
N
D000818:Animals; D016782:Apicomplexa / Q000235:genetics / Q000276:immunology; D007117:Immunization, Secondary / Q000379:methods*; D011528:Protozoan Infections / Q000517:prevention & control; D016052:Protozoan Vaccines / Q000008:administration & dosage*; D016794:Theileria / Q000235:genetics / Q000276:immunology*; D013801:Theileriasis / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000008:administration & dosage*
D016052:Protozoan Vaccines; D014614:Vaccines, Synthetic