title: Detection of porcine circovirus 2 (PCV-2) DNA by nested PCR from formalin-fixed tissues of post-weaning multisystemic wasting syndrome (PMWS) pigs in Thailand.
abstract: A retrospective study for the detection of porcine circovirus 2 (PCV-2) DNA was conducted by nested PCR method in 16 cases of swine post-weaning multisystemic wasting syndrome (PMWS) in Thailand. Histopathology showed characteristic lesions of PMWS and intracytoplasmic viral inclusion bodies in macrophages infiltrating in lymphoid tissues. PCV-2 DNA was detected from formalin-fixed and/or formalin-fixed paraffin-embedded tissues from all pigs with PMWS. The amplified products were digested with Hae III.
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Wijit
authors.initials: W
authors.lastname: Kiatipattanasakul-Banlunara
authors.forename: Rachod
authors.initials: R
authors.lastname: Tantilertcharoen
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Suzuki
authors.forename: Stella Maris
authors.initials: SM
authors.lastname: Albarenque
authors.forename: Roongroje
authors.initials: R
authors.lastname: Thanawongnuwech
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakayama
authors.forename: Kunio
authors.initials: K
authors.lastname: Doi
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000662:veterinary / Q000821:virology*; D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis*; D016133:Polymerase Chain Reaction; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000821:virology*; D019282:Wasting Syndrome / Q000662:veterinary / Q000821:virology*
chemical_list: D004279:DNA, Viral