PQBP-1 increases vulnerability to low potassium stress and represses transcription in primary cerebellar neurons.
PQBP-1 is a polyglutamine tract binding protein implicated in transcription. We previously reported that PQBP-1 and mutant ataxin-1, product of the spinocerebellar atrophy type 1 (SCA1) causative gene, cooperatively induce cell death in culture cells. Simultaneously, we showed that mutant ataxin-1 promoted interaction between PQBP-1 and RNA polymerase II and enhanced repression of the basal transcription by PQBP-1. In this study, we have examined the effects of overexpression of PQBP-1 to the primary-cultured cerebellar neurons. Our results indicate that overexpression of PQBP-1 inhibits the basal transcription in cerebellar neurons and increases their vulnerability to low potassium conditions.
Enokido
Y
Y
Division of Protein Biosynthesis, Institute for Protein Research, Osaka University, Japan.
Maruoka
H
H
Hatanaka
H
H
Kanazawa
I
I
Okazawa
H
H
D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004268:DNA-Binding Proteins; D017930:Genes, Reporter; D051379:Mice; D008808:Mice, Inbred CBA; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C492272:Pqbp1 protein, mouse; D011188:Potassium