title: Intratumoral low-volume jet-injection for efficient nonviral gene transfer.
abstract: Jet-injection has become an applicable technology among other established nonviral delivery systems, such as particle bombardment or in vivo electroporation. The low-volume jet injector employed in this study uses compressed air to inject solutions of 1.5-10 microL containing naked DNA into the desired tissue. The novel design of this prototype makes multiple jet-injections possible. Therefore, repeated jet-injections into one target tissue can be performed easily. This jet-injector hand-held system was used for the direct in vivo gene transfer of plasmid DNA into tumors to achieve efficient expression of reporter genes (beta-galactosidase, green fluorescent protein [GFP]) and of therapeutic genes (TNF-alpha) in different tumor models. The study presented here revealed the key parameters of efficient in vivo jet-injection (jet-injection volume, pressure, jet penetration, DNA stability) to define the optimal conditions for a jet-injection-aided nonviral gene therapy.
authors.affiliation: Max-Delbrück-Center for Molecular Medicine, Berlin, Germany. wowalt@mdc-berlin.de
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Walther
authors.forename: Ulrike
authors.initials: U
authors.lastname: Stein
authors.forename: Iduna
authors.initials: I
authors.lastname: Fichtner
authors.forename: Carsten
authors.initials: C
authors.lastname: Voss
authors.forename: Torsten
authors.initials: T
authors.lastname: Schmidt
authors.forename: Martin
authors.initials: M
authors.lastname: Schleef
authors.forename: Thomas
authors.initials: T
authors.lastname: Nellessen
authors.forename: Peter M
authors.initials: PM
authors.lastname: Schlag
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods*; D049452:Green Fluorescent Proteins; D006801:Humans; D007277:Injections, Jet / Q000379:methods*; D007763:Lac Operon; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009369:Neoplasms; D010957:Plasmids / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D008164:Luminescent Proteins; D014409:Tumor Necrosis Factor-alpha; D049452:Green Fluorescent Proteins