Spectrophotometric determination of enoxacin as ion-pairs with bromophenol blue and bromocresol purple in bulk and pharmaceutical dosage form.
Three simple, accurate and sensitive spectrophotometric methods were developed for determination of enoxacin. The methods based on extraction of this drug into chloroform as ion pairs with sulphonphthalein dyes as bromophenol blue and bromocresol purple. The optimum conditions of the reactions were studied and optimized. The absorbance of yellow products was measured at 412 nm for enoxacin-bromophenol blue and 410 nm for enoxacin-bromocresol purple. Linearity ranges were found to be 2.0-20.0 microg ml(-1) for enoxacin-bromophenol blue and 0.77-17.62 microg ml(-1) for enoxacin-bromocresol purple. The detection limits were found to be 0.084 microg ml(-1) and 0.193 microg ml(-1) for enoxacin-bromophenol blue and enoxacin-bromocresol purple, respectively. The composition of the ion pairs was found 1:1 by Job's method. The developed methods were applied successfully for the determination of this drug in pharmaceutical preparation. The data obtained by developed methods were compared with the spectrophotometric method in literature. No differences were found statistically.
Süslü
Incilay
I
Department of Analytical Chemistry, Faculty of Pharmacy, University of Hacettepe, 06100 Ankara, Turkey. isuslu@hacettepe.edu.tr
Tamer
Ayla
A
D001962:Bromcresol Purple / Q000737:chemistry; D001978:Bromphenol Blue / Q000737:chemistry; D004304:Dosage Forms; D015365:Enoxacin / Q000032:analysis* / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D004304:Dosage Forms; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D001978:Bromphenol Blue; D001962:Bromcresol Purple; D015365:Enoxacin