Liver- and lobe-selective gene transfection following the instillation of plasmid DNA to the liver surface in mice.
The present study has undertaken the liver- and lobe-selective gene transfections following the instillation of plasmid DNA (pDNA) to the liver surface in mice. The luciferase levels produced in the applied (left) liver lobe at 6 h after liver surface instillation of pDNA were significantly higher than those produced in the other tissues assayed, and ranged from 8.5-fold higher in other liver lobes to 320-fold higher in other tissues. After small intestine surface instillation of pDNA, the gene expression was a little detected in the tissues assayed. Following liver surface instillation of pDNA at a time from 2 to 48 h or at a volume from 15 to 120 microl, the gene expressions of the applied liver lobe were always significantly higher than those of other liver lobes and other tissues. We demonstrated the novel liver- and lobe-selective gene transfection utilizing the instillation to the liver surface.
Kawakami
Shigeru
S
School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. kawakami@net.nagasaki-u.ac.jp
Hirayama
Ryu
R
Shoji
Keiko
K
Kawanami
Rie
R
Nishida
Koyo
K
Nakashima
Mikiro
M
Sasaki
Hitoshi
H
Sakaeda
Toshiyuki
T
Nakamura
Junzo
J
D000818:Animals; D005786:Gene Expression Regulation; D007421:Intestine, Small / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D010957:Plasmids / Q000008:administration & dosage / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D014162:Transfection
D008156:Luciferases