title: sigma(2) Receptors regulate changes in sphingolipid levels in breast tumor cells.
abstract: sigma(2) Receptors induce apoptosis in various cell types. The sphingolipid, ceramide as well as the sphingoid bases are involved in cell proliferation. Sphingolipids of MCF-7/Adr- and T47D breast tumor cells were metabolically radiolabeled. The sigma(2) receptor agonists (+)-1R,5R-E-8-(3,4-dichlorobenzylidene)-5-(3-hydroxyphenyl)-2-methylmorphan-7-one (CB-184) and 1S,2R-(--)-cis-N-[2-(3,4-dichlorophenyl)ethyl]-N-methyl-2-(1-pyrrolidinyl)-cyclohexylamine (BD737) caused dose-dependent increases in [(3)H]ceramide, with concomitant decreases in [(3)H]sphingomyelin. Both effects were attenuated by the novel sigma(2) receptor antagonist, N-phenethylpiperidine oxalate (AC927). sigma(2) Receptors may produce effects on cell growth and apoptosis by regulating the sphingolipid pathway.
authors.affiliation: Department of Pharmacology, Howard University College of Medicine, Washington, DC 20059, USA.
authors.forename: Keith W
authors.initials: KW
authors.lastname: Crawford
authors.forename: Andrew
authors.initials: A
authors.lastname: Coop
authors.forename: Wayne D
authors.initials: WD
authors.lastname: Bowen
mesh_terms: D001597:Benzylidene Compounds / Q000494:pharmacology; D001943:Breast Neoplasms; D002455:Cell Division; D003514:Cyclohexylamines / Q000494:pharmacology; D006801:Humans; D007223:Infant; D009019:Morphinans / Q000494:pharmacology; D010070:Oxalates / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D011759:Pyrrolidines / Q000494:pharmacology; D017480:Receptors, sigma / Q000819:agonists / Q000502:physiology*; D013109:Sphingomyelins / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D001597:Benzylidene Compounds; C096583:CB 182; D003514:Cyclohexylamines; D009019:Morphinans; C470324:N-phenethylpiperidine oxalate; D010070:Oxalates; D010880:Piperidines; D011759:Pyrrolidines; D017480:Receptors, sigma; D013109:Sphingomyelins; C075567:BD 737