title: Secretion polarity of interferon-beta in epithelial cell lines.
abstract: Epithelial cells are an attractive target for local gene delivery in gene therapy for which cytokine genes such as interferon (IFN) genes are promising. However, how the secretion of the gene products is regulated in epithelial cells has been insufficiently investigated. Here, we have studied the secretion polarity of IFN-beta expressed via gene transfection in mouse epithelial Pam-T cells on a bicameral culture system. In transient expression, IFN-beta was predominantly secreted from the cell membrane side on which the transfection was carried out. Meanwhile, the secretion of constitutive IFN-beta from stable transformants was apparently unpolarized. Interestingly, the transformants displayed a polarized secretion of transiently expressed IFN-beta in a transfection-side-dependent manner, their stable IFN-beta secretion remaining unpolarized. These results suggest that epithelial cells have at least dual protein sorting-secretion pathways, transient and stable, for the same secretory proteins, such as IFNs.
authors.affiliation: Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Kiyo
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Masato
authors.initials: M
authors.lastname: Maruyama
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Yamashita
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Takakura
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Hashida
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D016899:Interferon-beta / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D021381:Protein Transport; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D016899:Interferon-beta