Lectin as a marker for staining and purification of embryonic pancreatic epithelium.
The embryonic pancreatic epithelium, and later the ductal epithelium, is known to give rise to the endocrine and exocrine cells of the developing pancreas, but no specific surface marker for these cells has been identified. Here, we utilized Dolichos Biflorus Agglutinin (DBA) as a specific marker of these epithelial cells in developing mouse pancreas. From the results of an immunofluorescence study using fluorescein-DBA and pancreatic specific cell markers, we found that DBA detects specifically epithelial, but neither differentiating endocrine cells nor acinar cells. We further applied this marker in an immunomagnetic separation system (Dynabead system) to purify these putative multi-potential cells from a mixed developing pancreatic cell population. This procedure could be applied to study differentiation and cell lineage selections in the developing pancreas, and also may be applicable to selecting pancreatic precursor cells for potential cellular engineering.
Kobayashi
Hiroyuki
H
Laboratory of Surgical Organogenesis, Children's Mercy Hospital, 2401 Gillham Road, Kansas City, MO 64108, USA.
Spilde
Troy L
TL
Li
Zhixing
Z
Marosky
Julie K
JK
Bhatia
Amina M
AM
Hembree
Mark J
MJ
Prasadan
Krishna
K
Preuett
Barry L
BL
Gittes
George K
GK
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002478:Cells, Cultured; D004848:Epithelium / Q000737:chemistry / Q000196:embryology; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D018189:Immunomagnetic Separation / Q000379:methods*; D037102:Lectins / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D010179:Pancreas / Q000737:chemistry / Q000166:cytology* / Q000196:embryology*; D010183:Pancreatic Ducts / Q000166:cytology / Q000196:embryology; D037121:Plant Lectins
D015415:Biomarkers; D005456:Fluorescent Dyes; D037102:Lectins; D037121:Plant Lectins; C036554:dolichos biflorus agglutinin