C-type lectins DC-SIGN and L-SIGN mediate cellular entry by Ebola virus in cis and in trans.
Ebola virus is a highly lethal pathogen responsible for several outbreaks of hemorrhagic fever. Here we show that the primate lentiviral binding C-type lectins DC-SIGN and L-SIGN act as cofactors for cellular entry by Ebola virus. Furthermore, DC-SIGN on the surface of dendritic cells is able to function as a trans receptor, binding Ebola virus-pseudotyped lentiviral particles and transmitting infection to susceptible cells. Our data underscore a role for DC-SIGN and L-SIGN in the infective process and pathogenicity of Ebola virus infection.
Alvarez
Carmen P
CP
Laboratory of Molecular Microbiology, Dept. of Microbiology, Hospital 12 de Octubre, 28041 Madrid, Spain.
Lasala
Fátima
F
Carrillo
Jaime
J
Muñiz
Oscar
O
Corbí
Angel L
AL
Delgado
Rafael
R
D015815:Cell Adhesion Molecules; D003713:Dendritic Cells / Q000378:metabolism; D029043:Ebolavirus / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D019169:Jurkat Cells; D037102:Lectins / Q000378:metabolism*; D037181:Lectins, C-Type; D009000:Monocytes; D011946:Receptors, Antigen / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014771:Virion / Q000378:metabolism; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015815:Cell Adhesion Molecules; D037102:Lectins; D037181:Lectins, C-Type; D011946:Receptors, Antigen; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C433029:CLEC4M protein, human