title: A colorimetric enzyme-linked on-bead assay for identification of synthetic substrates of protein tyrosine kinases.
abstract: Protein tyrosine kinases play key roles in the progression of numerous human diseases including several types of cancers. We report here a simple colorimetric assay for tyrosine kinase activity employing synthetic peptide substrates prepared on Tentagel synthesis beads. Phosphorylation of compounds on beads was detected with an antiphosphotyrosine antibody complexed with a secondary antibody-alkaline phosphatase conjugate. This assay may prove useful for the identification and characterization of synthetic substrates of this important class of enzymes.
authors.affiliation: Department of Chemistry, The Pennsylvania State University, University Park 16802, USA.
authors.forename: Scott E
authors.initials: SE
authors.lastname: Martin
authors.forename: Blake R
authors.initials: BR
authors.lastname: Peterson
mesh_terms: D003124:Colorimetry / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D010455:Peptides; D011505:Protein-Tyrosine Kinases