Patterns of immunoglobulin staining in paraffin-embedded malignant lymphomas.
The demonstration of immunoglobulin light chain restriction in paraffin-embedded B cell lymphomas is a capricious and difficult procedure that has been abandoned by many diagnostic laboratories. Using a combination of microwave antigen retrieval performed in a pressure cooker and proteolytic digestion with trypsin, we were able to demonstrate immunoglobulin light chain restriction in 66 B cell lymphomas comprising 25 follicular lymphomas, 29 diffuse large cell lymphomas, 6 small lymphocytic lymphomas, 2 mantle cell lymphomas, 1 nodal marginal zone lymphoma, 1 Burkitt lymphoma, 1 hairy cell leukemia, and 1 plasmacytoma. There was concordance of results in 13 cases in which flow cytometry immunoglobulin analyzes were performed. Perinuclear staining was demonstrated in all cases with dot-like staining of the Golgi present in 23 cases. Perinuclear staining occurred in combination with cytoplasmic staining in 12 cases and membrane staining in 8 cases with 12 lymphomas showing more than two patterns of staining.
Leong
Anthony S Y
AS
Division of Anatomical Pathology, Hunter Area Pathology Services, University of Newcastle, New South Wales, Australia. aleong@mail.newcastle.edu.au
Yin
Hui
H
Hafajee
Zenobia
Z
D005434:Flow Cytometry; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology*; D016393:Lymphoma, B-Cell / Q000145:classification / Q000276:immunology* / Q000473:pathology; D016612:Paraffin Embedding; D013194:Staining and Labeling
D007136:Immunoglobulins