title: Could induced apoptosis of human granulosa cells predict in vitro fertilization-embryo transfer outcome? A preliminary study of 25 women.
abstract: OBJECTIVE
  The aim of this study was to investigate the relationship between induced apoptosis of human luteinized granulosa cells (GCs) and in vitro fertilization (IVF) outcome.
  
  
  METHODS
  We induced apoptosis with interferon gamma and an anti-human Fas antibody in cultured GCs isolated from follicular fluids coming from 25 different women undergoing 25 consecutive IVF cycles. After examination of 1000 GCs stained by DAPI with a fluorescent microscope, we determined the percentages of apoptotic GCs. Ovarian, endometrial and IVF parameters were recorded for every woman.
  
  
  RESULTS
  We classified the women according to their induced GCs apoptosis percentages in two groups. Group 1 with a low percentage of apoptotic GCs (11.6+/-4.8%) had a significantly higher pregnancy rate (P<0.05) than group 2 with a high percentage of apoptotic GCs (59.5+/-14.8%). No other statistically significant differences were observed.
  
  
  CONCLUSIONS
  Resistance of human GCs to apoptosis might be implicated in IVF outcome.
authors.affiliation: Service d'Histologie, Embryologie et Cytogénétique, CHU Bichat-Claude Bernard, 46 rue Henri-Huchard, 75877 Cedex 18, Paris, France. histo.survgen@bch.ap-hop-paris.fr
authors.forename: Christophe
authors.initials: C
authors.lastname: Sifer
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Bénifla
authors.forename: Annie-France
authors.initials: AF
authors.lastname: Bringuier
authors.forename: Raphael
authors.initials: R
authors.lastname: Porcher
authors.forename: Grazielle
authors.initials: G
authors.lastname: Blanc-Layrac
authors.forename: Patrick
authors.initials: P
authors.lastname: Madélénat
authors.forename: Gerard
authors.initials: G
authors.lastname: Feldmann
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D004624:Embryo Transfer / Q000706:statistics & numerical data*; D004715:Endometriosis / Q000473:pathology; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000187:drug effects / Q000473:pathology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D011237:Predictive Value of Tests; D011247:Pregnancy; D018873:Pregnancy Rate; D016896:Treatment Outcome; D019014:fas Receptor / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D019014:fas Receptor; D007371:Interferon-gamma