title: Group B streptococcus (GBS) modifies macrophage phosphatidylserine metabolism during induction of apoptosis.
abstract: Group B streptococcus (GBS) induced macrophage apoptosis by which it could avoid host defence mechanisms. Macrophages, which constitutively express phosphatidylserine (PtdSer) on the outer leaflet of plasma membrane, increased PtdSer exposure during GBS-induced apoptosis. Induction of apoptosis decreased PtdSer radioactivity of macrophages incubated with [(3)H]serine. The effect appeared not due to increasing conversion of PtdSer to phosphatidylethanolamine or phosphatidylcholine nor to the release of radioactive membrane vesicles. The radioactivity in lysoPtdSer was also reduced. These results confirm that induction of apoptosis involves a modification of PtdSer metabolism and point out the typical features of the GBS-induced apoptosis with respect to other models of apoptosis.
authors.affiliation: Department of Internal Medicine, Biochemistry Section, University of Perugia, 06122 Perugia, Italy.
authors.forename: Sandra
authors.initials: S
authors.lastname: Buratta
authors.forename: Katia
authors.initials: K
authors.lastname: Fettucciari
authors.forename: Raffaela
authors.initials: R
authors.lastname: Mambrini
authors.forename: Ilaria
authors.initials: I
authors.lastname: Fetriconi
authors.forename: Pierfrancesco
authors.initials: P
authors.lastname: Marconi
authors.forename: Rita
authors.initials: R
authors.lastname: Mozzi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology; D008297:Male; D051379:Mice; D019884:Nitrogenous Group Transferases / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism*; D013292:Streptococcus agalactiae / Q000254:growth & development / Q000502:physiology*; D013997:Time Factors
chemical_list: D010718:Phosphatidylserines; D019884:Nitrogenous Group Transferases; C022370:phospholipid serine base exchange enzyme