Characterization of a recombinant mu-class glutathione S-transferase from Taenia solium.
A Taenia solium larval glutathione S-transferase fraction (SGSTF), composed of two proteins with Mr 25,500 (SGSTM1) and 26,500 (SGSTM2), was purified by GSH-sepharose. Its N-terminal sequence analysis revealed that both proteins are related to mammalian mu-class GST enzymes. A cDNA clone coding for SGSTM1 was isolated and the amino acid sequence analysis showed close identity with two Echinococcus GSTs and also high identity with several mu-class GSTs that have been reported. In addition, SGSTM1 presents a similar structure to mu-class GSTs, including the mu loop. The recombinant SGSTM1 is a dimeric protein with enzymatic properties clearly related to mammalian mu-class GSTs. Western blot studies indicated that SGSTM1 is not antigenically related to SGSTM2 or mammalian GSTs from rabbit, pig and rat livers. Immunization with SGSTF and SGSTM2 was highly effective in reducing cysticerci load in murine cysticercosis. In contrast, no protection was obtained using native SGSTM1 and recombinant SGSTM1 as immunogens.
Vibanco-Pérez
N
N
Depto. Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico DF.
Jiménez
L
L
Mendoza-Hernández
G
G
Landa
A
A
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003551:Cysticercosis / Q000517:prevention & control; D018509:DNA, Helminth / Q000032:analysis / Q000235:genetics; D005260:Female; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007114:Immunization; D007527:Isoenzymes; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D041201:Taenia solium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D014612:Vaccines / Q000008:administration & dosage
D018509:DNA, Helminth; D007527:Isoenzymes; D011994:Recombinant Proteins; D014612:Vaccines; D005982:Glutathione Transferase