Expression of the full-length and 3'-spliced cry1Ab gene in the 135-kDa crystal protein minus derivative of Bacillus thuringiensis subsp. kyushuensis.
Bacillus thuringiensis produces a 130-135-kDa insecticidal protein in the form of bipyramidal crystal which is toxic to lepidopteran larvae. Part of the C-terminal region of the native Cry1Ab was replaced by a heterologous sequence of Cry11Aa C-terminus to get a 3'-spliced cry1Ab gene. The full-length cry1Ab and 3'-spliced cry1Ab, which were both cloned into the E. coli-B. thuringiensis shuttle expression vector pHZB1, were expressed in a 135-kDa crystal protein minus derivative of B. thuringiensis subsp. kyushuensis (4U1-Cry(-135)). The crystal shape of Cry1Ab proteins from both recombinants was regularly bipyramidal, while the crystal size of the intact Cry1Ab was approximately fivefold larger than the 3'-spliced Cry1Ab. In addition, these two kinds of Cry1Ab proteins had similar toxicity against Argyrogramma agnata larvae.
Yu
Jianxiu
J
State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, Guangzhou, 510275, People's Republic of China.
Xie
Ruiyu
R
Tan
Le
L
Xu
Wei
W
Zeng
Shaoling
S
Chen
Jianwu
J
Tang
Mujin
M
Pang
Yi
Y
D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity; D001427:Bacterial Toxins; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D006460:Hemolysin Proteins; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010957:Plasmids; D011994:Recombinant Proteins; D015183:Restriction Mapping
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis