title: Cooperative effect of artificial chaperones and guanidinium chloride on lysozyme renaturation at high concentrations.
abstract: It has been recognized that the artificial chaperone system, cetyltrimethylammonium bromide and beta-cyclodextrin, is effective for enhancing protein renaturation. In this work, we studied the effect of the artificial chaperone system and guanidinium chloride (GdmCl) on the oxidative renaturation of lysozyme at 0.21-1.05 mg/mL, and a kinetic model based on the competition between protein folding and aggregation was employed to express the renaturation process. The refolding rate constant increased, while the aggregation rate constant decreased, with increasing concentration of the artificial chaperones. With increasing GdmCl concentration (0.28-2 M), both rate constants decreased, but there existed a specific GdmCl concentration that maximized the ratio of the two rate constants and thus the renaturation yield. The results obviously indicated the cooperative effect of GdmCl and the artificial chaperones on enhancing protein renaturation.
authors.affiliation: Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Dong
authors.forename: Jin-Hui
authors.initials: JH
authors.lastname: Shi
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
mesh_terms: D019791:Guanidine / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D018832:Molecular Chaperones / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry*; D020673:Protein Renaturation
chemical_list: D018832:Molecular Chaperones; D009113:Muramidase; D019791:Guanidine