title: Recognition of pathogenically relevant house dust mite hypersensitivity in adults with atopic dermatitis: a new approach?
abstract: BACKGROUND
  The pathogenic importance of the ubiquitous house dust mite, Dermatophagoides pteronyssinus (Dp), in atopic dermatitis is unclear.
  
  
  OBJECTIVE
  We aimed to explore the relevance of Dp hypersensitivity in adult patients with atopic dermatitis by using an in vivo topical challenge method and in vitro assays for T-cell reactivity.
  
  
  METHODS
  Dp and control skin prick test solutions were applied to the cubital fossae of 20 patients twice daily for 4 days; the severity of dermatitis and pruritus in the challenge sites were determined before and after testing. The same solutions were used in PBMC proliferation assays that included 10% fresh, autologous serum, the latter aimed at maximizing IgE facilitated allergen presentation.
  
  
  RESULTS
  Although most patients had markedly elevated Dp-specific serum IgE levels, only 6 of 20 patients developed increases in cubital fossa dermatitis severity and pruritus scores that were greater at sites of application of Dp solution than at control sites. In addition, PBMC proliferation in response to Dp solution in the presence of autologous serum was significantly greater in the in vivo challenge-positive patients than in those who did not respond to challenge. A subgroup of patients (7/20) also developed transient but pronounced contact urticaria at sites of Dp application.
  
  
  CONCLUSIONS
  These findings suggest that hypersensitivity to Dp might be clinically relevant in approximately one third of the adult atopic dermatitis population studied. They also point to methods of identifying patients who might respond to house dust avoidance measures.
authors.affiliation: Division of Dermatology, University of Leicester, United Kingdom.
authors.forename: Dev
authors.initials: D
authors.lastname: Shah
authors.forename: Jonathan
authors.initials: J
authors.lastname: Hales
authors.forename: Dawn
authors.initials: D
authors.lastname: Cooper
authors.forename: Richard
authors.initials: R
authors.lastname: Camp
mesh_terms: D000328:Adult; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D039741:Antigens, Dermatophagoides; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002455:Cell Division; D003876:Dermatitis, Atopic / Q000276:immunology*; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000276:immunology*; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D011537:Pruritus / Q000276:immunology; D012882:Skin Tests; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D007073:Immunoglobulin E