Postprandial changes in serum unconjugated bile acid concentrations in healthy beagles.
OBJECTIVE
To investigate postprandial changes in serum concentrations of unconjugated bile acids in healthy Beagles.


METHODS
7 healthy Beagles.


METHODS
Blood samples were obtained from dogs at regular intervals up to 8 hours after consumption of a meal. Serum concentrations of 5 unconjugated bile acids were determined at each time point, using gas chromatography-mass spectrometry with selected ion monitoring.


RESULTS
Total serum unconjugated bile acid concentration was significantly increased, relative to baseline values, at 360, 420, and 480 minutes after feeding. Unconjugated cholic acid was significantly increased at 360, 420, and 480 minutes. The proportion of total unconjugated bile acids represented by cholic acid was significantly increased at 240 to 480 minutes. Deoxycholic acid was significantly increased at 360 and 420 minutes. Chenodeoxycholic acid was significantly increased at 360 to 480 minutes. Lithocholic acid was significantly increased at 180 minutes, whereas no significant changes in ursodeoxycholic acid were detected at any time point.


CONCLUSIONS
Healthy Beagles had significant increases in serum concentrations and changes in the profile of unconjugated bile acids after a meal. These increases persisted > 8 hours, indicating that prolonged withholding of food is necessary to avoid the risk of a false-positive diagnosis when assessing serum unconjugated bile acid concentrations in dogs.
Ruaux
Craig G
CG
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, Texas A & M University, College Station 77843-4474, USA.
Steiner
Jorg M
JM
Williams
David A
DA
D000818:Animals; D001647:Bile Acids and Salts / Q000097:blood*; D004285:Dogs / Q000097:blood*; D005247:Feeding Behavior; D005260:Female; D008297:Male; D019518:Postprandial Period / Q000502:physiology*; D013997:Time Factors
D001647:Bile Acids and Salts