title: Ultraviolet light-induced changes in the glucose-6-phosphate dehydrogenase activity of porcine corneas.
abstract: OBJECTIVE
  Corneas have the high activity of the pentose phosphate pathway. To clarify the relevance of the pentose phosphate pathway to their antioxidant defense, we examined the activity of glucose-6-phosphate dehydrogenase (G6PDH), the rate-determining enzyme of the pathway, in corneas exposed to ultraviolet light (UV).
  
  
  METHODS
  Fresh porcine eye globes were exposed to ultraviolet light A (UVA) or ultraviolet light C (UVC), and the tear film-removed eye globes to UVA. After UV exposure, the corneas were dissected from the eye globes and extracted with a saline solution, and the G6PDH activity in the extract was assayed. The G6PDH activity of unilateral opaque corneas, their paired, transparent corneas, and normal corneas, all of which were obtained from fresh, UV-unexposed eye globes, and the L-lactate dehydrogenase (LDH) activity of these opaque and normal corneas also were assayed.
  
  
  RESULTS
  The G6PDH activity of corneas increased with UVA exposure, and decreased with long-term UVC exposure, although it increased with short-term UVC exposure. A UV-blocking contact lens screened corneas from the UVA-induced increase. Removal of the tear film enhanced the UVC-induced decrease. The G6PDH activity of unilateral opaque corneas was lower than that of paired, transparent corneas or normal corneas, all of which were obtained from fresh, UV-unexposed eye globes. The LDH activity of the opaque corneas was much higher than that of the normal corneas.
  
  
  CONCLUSIONS
  Exposure of corneas to UVA or a small dose of UVC enhances the G6PDH activity, i.e., the pentose phosphate pathway. This activity enhancement may play an important role in corneal antioxidant defense against UV-induced oxidative stress. However, exposure of corneas to large doses of UVC appears to damage the pathway.
authors.affiliation: Department of Biology, Faculty of Science and High Technology Research Center, Konan University, 8-9-1 Okamoto, Higashinadaku, Kobe, Hyogo 658-8501, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Tsubai
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Matsuo
mesh_terms: D000818:Animals; D003315:Cornea / Q000201:enzymology / Q000528:radiation effects*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006019:Glycolysis / Q000528:radiation effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010427:Pentose Phosphate Pathway / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000201:enzymology*; D013552:Swine; D014466:Ultraviolet Rays
chemical_list: D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase