title: Membrane-facilitated bioproduction of 3-methylcatechol in an octanol/water two-phase system.
abstract: Bioproduction of 3-methylcatechol from toluene by Pseudomonas putida MC2 was studied in the presence of an additional 1-octanol phase. This solvent was used to supply the substrate and extract the product, in order to keep the aqueous concentrations low. A hollow-fibre membrane kept the octanol and aqueous phase separated to prevent phase toxicity towards the bacterium. Volumetric production rates increased approximately 40% as compared to two-phase 3-methylcatechol production with direct phase contact. Preliminary investigations on downstream processing of 3-methylcatechol showed that 1 M of sodium hydroxide selectively extracted the disodium salt of 3-methylcatechol into an aqueous phase.
authors.affiliation: Food and Bioprocess Engineering Group, Department of Agrotechnology and Food Sciences, Wageningen University, PO Box 8129, 6700 EV, Wageningen, The Netherlands. leonie.husken@algemeen.pk.wau.nl
authors.forename: Leonie E
authors.initials: LE
authors.lastname: Hüsken
authors.forename: Mirjam
authors.initials: M
authors.lastname: Oomes
authors.forename: Karin
authors.initials: K
authors.lastname: Schroën
authors.forename: Johannes
authors.initials: J
authors.lastname: Tramper
authors.forename: Jan A M
authors.initials: JA
authors.lastname: de Bont
authors.forename: Rik
authors.initials: R
authors.lastname: Beeftink
mesh_terms: D018533:Biomass; D019149:Bioreactors; D002396:Catechols / Q000032:analysis / Q000138:chemical synthesis* / Q000378:metabolism*; D008567:Membranes, Artificial; D008954:Models, Biological; D000442:Octanols / Q000737:chemistry*; D011126:Polypropylenes; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*; D013499:Surface Properties; D014050:Toluene / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D002396:Catechols; D008567:Membranes, Artificial; D000442:Octanols; D011126:Polypropylenes; D014867:Water; C031136:3-methylcatechol; D014050:Toluene