Delayed anti-kappa response in kappa-deficient mice after neonatal, oral immunization with kappa-containing IgG.
Antibody responses to kappa (kappa)-light (L) chain are absent in normal (Ckappa+/+) animals because of tolerance due to the abundance of kappa-L chains expressed on more than 95% of all B cells and serum Ig.When heterozygous kappa-sufficient (Ckappa+/-) females are bred with homozygous kappa-deficient (Ckappa-/-) males, half of their offspring will become kappa-deficient but have received kappa-L chain containing maternal Ig, mainly IgG and IgA, through placental and intestinal transmission. The kappa-containing maternal Ig persists for more than 2 months in the circulation of the offspring. Starting from weeks 15 to 20 of age, a spontaneous antibody response towards the maternal kappa-L chains can be recorded. The time of onset, as well as the magnitude of the responses differ among individuals of the same litter. Invariably, once a response has been initiated, it transits into an IgG-type of response, which upon injection with kappa-containing protein shows the features of a secondary type of immune response.
Bengtsson
Otti
O
Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Daggfeldt
Annika
A
Andersson
Jan
J
Grönvik
Kjell-Olov
KO
D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000276:immunology*; D008297:Male; D051379:Mice
D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains