Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis.
Mechanical wounding not only damages plant tissues, but also provides pathways for pathogen invasion. To understand plant responses to wounding at a genomic level, we have surveyed the transcriptional response of 8,200 genes in Arabidopsis plants. Approximately 8% of these genes were altered by wounding at steady-state mRNA levels. Studies of expression patterns of these genes provide new information on the interactions between wounding and other signals, including pathogen attack, abiotic stress factors, and plant hormones. For example, a number of wound-responsive genes encode proteins involved in pathogen response. These include signaling molecules for the pathogen resistance pathway and enzymes required for cell wall modification and secondary metabolism. Many osmotic stress- and heat shock-regulated genes were highly responsive to wounding. Although a number of genes involved in ethylene, jasmonic acid, and abscisic acid pathways were activated, many in auxin responses were suppressed by wounding. These results further dissected the nature of mechanical wounding as a stress signal and identified new genes that may play a role in wounding and other signal transduction pathways.
Cheong
Yong Hwa
YH
Department of Plant and Microbial Biology, University of California-Berkeley, Berkeley, CA 94720, USA.
Chang
Hur-Song
HS
Gupta
Rajeev
R
Wang
Xun
X
Zhu
Tong
T
Luan
Sheng
S
D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology; D005030:Ethylenes / Q000494:pharmacology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018506:Gene Expression Regulation, Plant; D006360:Heat-Shock Proteins / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics; D007210:Indoleacetic Acids / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D009997:Osmotic Pressure; D054883:Oxylipins; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013314:Stress, Mechanical
D003517:Cyclopentanes; D005030:Ethylenes; D006360:Heat-Shock Proteins; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C011006:jasmonic acid; D000040:Abscisic Acid; C036216:ethylene