title: Multiresidue determination of (fluoro)quinolone antibiotics in swine kidney using liquid chromatography-tandem mass spectrometry.
abstract: New antibiotics were recently developed, among which are the (fluoro)quinolones. This paper presents an analytical method which allows the determination of 11 (fluoro)quinolones in swine kidneys: norfloxacin, ofloxacin, cinoxacin, oxolinic acid, nalidixic acid, flumequine, enrofloxacin, enoxacin, ciprofloxacin, danofloxacin and marbofloxacin. The procedure involves a rapid and efficient pre-treatment by solid-phase extraction (recoveries 83-98%), followed by the sensitive and selective determination of all compounds in a single run using LC-ESI-MS-MS. Multiple reaction monitoring (MRM) was used for selective detection of each (fluoro)quinolone. Quinine was selected as internal standard. The accuracy of the method, expressed as recovery, was between 89 and 109%; the repeatability had a maximum RSD lower than 15%. The limits of detection (LOD) were much lower than the respective Maximum Residue Limits (MRL)/4.
authors.affiliation: European Commission, Joint Research Center, Institute for Reference Materials and Measurements Geel, Retieseweg, Belgium.
authors.forename: Géry
authors.initials: G
authors.lastname: van Vyncht
authors.forename: Amaya
authors.initials: A
authors.lastname: Jànosi
authors.forename: Guy
authors.initials: G
authors.lastname: Bordin
authors.forename: Brigitte
authors.initials: B
authors.lastname: Toussaint
authors.forename: Guy
authors.initials: G
authors.lastname: Maghuin-Rogister
authors.forename: Edwin
authors.initials: E
authors.lastname: De Pauw
authors.forename: Adela Rosa
authors.initials: AR
authors.lastname: Rodriguez
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D024841:Fluoroquinolones; D007668:Kidney / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones