title: A more sensitive method for the quantitation of genomic DNA by Alu amplification.
abstract: Current procedures for human DNA quantitation reach their limit at 150 pg DNA, which is above the limit of the PCR profiling range using Profiler-Plus (Applied Biosystems, CA). This study tested the potential for the use of primate specific Alu sequences in forensic science for the sensitive detection and quantitaion of DNA. A fluorescently labelled primer pair was designed enabling high efficiency amplification of the core Alu sequence within primate DNA. Quantitation was performed by measurement of fluorescence intensity and comparison to a series of standard template DNA amounts via the construction of a standard curve. The new Alu-based quantitation protocol developed has shown its feasibility in more sensitively quantitating (100-2.5 pg) unknown amounts of human DNA for forensic use. The method is compatible with the use and throughput of current forensic procedures.
authors.affiliation: Forensic Science Centre, Adelaide, SA, Australia.
authors.forename: Melanie E
authors.initials: ME
authors.lastname: Sifis
authors.forename: Katrin
authors.initials: K
authors.lastname: Both
authors.forename: Leigh A
authors.initials: LA
authors.lastname: Burgoyne
mesh_terms: D020087:Alu Elements / Q000235:genetics*; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA