title: Simple and rapid quantification of the non-nucleoside reverse transcriptase inhibitors nevirapine, delavirdine, and efavirenz in human blood plasma using high-performance liquid chromatography with ultraviolet absorbance detection.
abstract: A simple reversed-phase high-performance liquid chromatography assay for the simultaneous quantitative determination of three HIV non-nucleoside reverse transcriptase inhibitors (nevirapine, delavirdine, and efavirenz) in human blood plasma is described. The method was validated over the range of 10 ng/ml to 50 microg/ml for nevirapine, 25 ng/ml to 25 microg/ml for delavirdine, and 10 ng/ml to 10 microg/ml for efavirenz. The method is accurate (average accuracies over eight concentrations ranging from 87.3 to 113%), and precise (within-day and between-day precision measures ranging from 0.12 to 7.9% and 0.26 to 5.9%, respectively). All three non-nucleoside reverse transcriptase inhibitors proved to be stable under various conditions. Due to its simplicity, this assay can readily be used for investigational or clinical monitoring of plasma concentrations.
authors.affiliation: Pharmacology Core, Center for AIDS Research, 808 Brinkhouse-Bullitt, CB #7525, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. naser2@med.unc.edu
authors.forename: Naser L
authors.initials: NL
authors.lastname: Rezk
authors.forename: Richard R
authors.initials: RR
authors.lastname: Tidwell
authors.forename: Angela D M
authors.initials: AD
authors.lastname: Kashuba
mesh_terms: D000480:Alkynes; D048588:Benzoxazines; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003521:Cyclopropanes; D020008:Delavirdine / Q000097:blood*; D015658:HIV Infections / Q000097:blood; D015497:HIV-1; D006801:Humans; D019829:Nevirapine / Q000097:blood*; D010078:Oxazines / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000097:blood*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000480:Alkynes; D048588:Benzoxazines; D003521:Cyclopropanes; D010078:Oxazines; D018894:Reverse Transcriptase Inhibitors; D019829:Nevirapine; D020008:Delavirdine; C098320:efavirenz