Circadian rhythms confer a higher level of fitness to Arabidopsis plants.
Circadian rhythms have been demonstrated in organisms across the taxonomic spectrum. In view of their widespread occurrence, the adaptive significance of these rhythms is of interest. We have previously shown that constitutive expression of the CCA1 (CIRCADIAN CLOCK ASSOCIATED 1) gene in Arabidopsis plants (CCA1-ox) results in loss of circadian rhythmicity. Here, we demonstrate that these CCA1-ox plants retain the ability to respond to diurnal changes in light. Thus, transcript levels of several circadian-regulated genes, as well as CCA1 itself and the closely related LHY, oscillate robustly if CCA1-ox plants are grown under diurnal conditions. However, in contrast with wild-type plants in which transcript levels change in anticipation of the dark/light transitions, the CCA1-ox plants have lost the ability to anticipate this daily change in their environment. We have used CCA1-ox lines to examine the effects of loss of circadian regulation on the fitness of an organism. CCA1-ox plants flowered later, especially under long-day conditions, and were less viable under very short-day conditions than their wild-type counterparts. In addition, we demonstrate that two other circadian rhythm mutants, LHY-ox and elf3, have low-viability phenotypes. Our findings demonstrate the adaptive advantage of circadian rhythms in Arabidopsis.
Green
Rachel M
RM
Department of Molecular, Cell and Developmental Biology, P.O. Box 160606, University of California-Los Angeles, Los Angeles, CA 90095-1606, USA.
Tingay
Sonia
S
Wang
Zhi-Yong
ZY
Tobin
Elaine M
EM
D000064:Acclimatization / Q000235:genetics / Q000502:physiology; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins; D002470:Cell Survival; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D003624:Darkness; D008027:Light; D017440:Photoperiod; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology; D018547:Plant Stems / Q000502:physiology; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D018749:RNA, Plant; D014157:Transcription Factors; C114425:CCA1 protein, Arabidopsis; C432430:At2g25930 protein, Arabidopsis; C113192:LHY protein, Arabidopsis