Detection of vivax malaria sporozoites naturally infected in Anopheline mosquitoes from endemic areas of northern parts of Gyeonggi-do (Province) in Korea.
We investigated population densities of mosquitoes infected with sporozoites in three highly epidemic areas of Josan-ri and Jangpa-ri (Paju City) and Dongjung-ri (Yeoncheon County) in Korea. Anopheline mosquitoes were collected from both indoors and outdoors by human baiting collection method during the period of the first week of June to the second week of September 1999. Total 13,296 female mosquitoes were collected and 8,650 (65.1%) were Anophelines. Thirty seven percent (3,199) of the Anopheline mosquitoes were captured outdoors and 63.9% (5,531) indoors. Employing a sandwich enzyme-linked immunosorbent assay (ELISA), we analyzed a total of 7,820 Anopheline mosquitoes and found that 7 Anopheline mosquitoes were infected with sporozoites. The positive rate in Josan-ri was 0.14% (5/3,500) and 0.15% (2/1,370) in Jangpa-ri. The total positive rate in all three surveyed areas was 0.09% (7/7,820). The mosquitoes infected with the sporozoites were detected on June 28th (n = 2), July 5th (n = 1), July 19th (n = 1), August 9th (n = 1), September 6th (n = 1), and the last one on September 13th (n = 1). They were all classified as Anopheles sinensis, which showed positive reaction in ELISA test. Therefore it might be concluded that Anopheles sinensis plays an important role in re-emerging malaria transmission in Korea.
Lee
Hyeong Woo
HW
Department of Medical Zoology, National Institute of Health, Seoul, 122-701 Korea.
Shin
E-Hyun
EH
Cho
Shin Hyeong
SH
Lee
Hee Il
HI
Kim
Chung Lim
CL
Lee
Wook Gyo
WG
Moon
Sung Ung
SU
Lee
Jong Soo
JS
Lee
Won Ja
WJ
Kim
Tong-Soo
TS
D000818:Animals; D009033:Culicidae / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007723:Korea / Q000453:epidemiology; D016780:Malaria, Vivax / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission; D010966:Plasmodium vivax / Q000302:isolation & purification*