title: Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon.
abstract: Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn5Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity.
authors.affiliation: Département de Biologie Moléculaire et Structurale, BBSI, UMR 5092 CNRS, CEA-Grenoble, CHU-Grenoble, Grenoble, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Dacheux
authors.forename: Olivier
authors.initials: O
authors.lastname: Epaulard
authors.forename: Arjan
authors.initials: A
authors.lastname: de Groot
authors.forename: Benoit
authors.initials: B
authors.lastname: Guery
authors.forename: Rozen
authors.initials: R
authors.lastname: Leberre
authors.forename: Ina
authors.initials: I
authors.lastname: Attree
authors.forename: Benoit
authors.initials: B
authors.lastname: Polack
authors.forename: Bertrand
authors.initials: B
authors.lastname: Toussaint
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D003550:Cystic Fibrosis / Q000382:microbiology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D004195:Disease Models, Animal; D000071677:Histidine Kinase; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009504:Neutrophils / Q000382:microbiology; D009876:Operon / Q000502:physiology*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism; D018410:Pneumonia, Bacterial / Q000382:microbiology; D052899:Pore Forming Cytotoxic Proteins; D011494:Protein Kinases / Q000378:metabolism; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D011768:Pyruvate Dehydrogenase Complex / Q000235:genetics / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C095092:ExsA protein, bacteria; C412007:PopB protein, Ralstonia solanacearum; D052899:Pore Forming Cytotoxic Proteins; D011768:Pyruvate Dehydrogenase Complex; D015534:Trans-Activators; C453599:pyruvate dehydrogenase multienzyme complex E1; D007770:L-Lactate Dehydrogenase; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D011494:Protein Kinases; D000071677:Histidine Kinase; D010749:Phosphoprotein Phosphatases; C080406:protein phosphatase 4