Construction and characterization of a single-chain antibody fragment derived from thymus of a patient with myasthenia gravis.
Pathogenic anti-acetylcholine receptor (AChR) antibodies in myasthenia gravis (MG) and the corresponding animal model, experimental autoimmune myasthenia gravis (EAMG), principally recognize the main immunogenic region (MIR) of the AChR. Bivalent anti-MIR antibodies binding to the alpha-subunits of AChR result in AChR loss by antigenic modulation and complement activation. Monovalent Fab and single-chain variable fragments (scFv) of pathogenic anti-AChR antibodies can interfere with AChR binding of the pathogenic antibodies. In the present study, scFv637 was constructed from its parental Fab637, previously isolated from a thymus-derived phage display library with specificity toward anti-MIR of human AChR (hAChR), by PCR amplification. Bacterial produced scFv637 was able to bind to hAChR in standard precipitation radioimmunoassay (RIA). ScFv637 also bound to monkey AChR in situ on monkey neuromuscular junctions as showed in immunohistochemical staining. Furthermore, scFv637 was capable of inhibiting the binding of its intact IgG637 and anti-MIR mAb35 binding to hAChR up to 32.9 and 73.0%, respectively demonstrated in a competitive ELISA, and of MG patient sera from up to 45.5% in a competitive RIA. Therefore, scFv637, easier for manipulation in improvement of affinity and stability compared with its parental Fab637, may serve as an alternative candidate for specific immunotherapy in MG.
Meng
Fanping
F
Department of Neurology, Maastricht Brain and Behaviour Institute, University of Maastricht, The Netherlands.
Stassen
Maurice H W
MH
Schillberg
Stefan
S
Fischer
Rainer
R
De Baets
Marc H
MH
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology; D000882:Haplorhini; D006801:Humans; D007128:Immunoglobulin Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D009157:Myasthenia Gravis / Q000276:immunology*; D011950:Receptors, Cholinergic / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology*
D000941:Antigens; D007128:Immunoglobulin Fragments; D011950:Receptors, Cholinergic