Endogenous expression of interleukin-8 and interleukin-10 in nasopharyngeal carcinoma cells and the effect of photodynamic therapy.
Interleukin-8 (Il-8) and IL-10 are cytokines associated with the Epstein Barr Virus (EBV), which is linked with nasopharyngeal cancer (NPC). In the present study, we investigated the endogenous production of IL-8 and IL-10 in vitro by two EBV-positive NPC cell lines, viz., HK1 and CNE-2. IL-8 was expressed by both cell lines although the level of IL-8 was 2-fold higher in supernatant from HK1 cells. Incubation with hypericin, a natural photosensitizer increased IL-8 significantly but only in HK1 cells. Hypericin-based photodynamic therapy (PDT) did not alter the expression of IL-8 levels. Il-10 was not constitutively expressed in either cell line and could not be induced by PDT. It is interesting that PDT which is known to upregulate IL-8 transcription via reactive oxygen species and activate the IL-10 promoter did not alter IL-8 levels in either of the NPC cell lines nor induced the production of IL-10.
Du
Hongyan
H
Department of Anatomy, National University of Singapore, Singapore.
Bay
Boon-Huat
BH
Mahendran
Ratha
R
Olivo
Malini
M
D000873:Anthracenes; D016207:Cytokines / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D010569:Perylene / Q000031:analogs & derivatives* / Q000378:metabolism; D010778:Photochemotherapy; D014407:Tumor Cells, Cultured
D000873:Anthracenes; D016207:Cytokines; D016209:Interleukin-8; D016753:Interleukin-10; D010569:Perylene; C004965:hypericin