title: Changes in the distribution and density of caveolin 3 molecules at the plasma membrane of mdx mouse skeletal muscles: a fracture-label electron microscopic study.
abstract: To analyze the molecular mechanism of the increased caveolin 3 activities in dystrophin-deficient muscles, we investigated three-dimensionally the changes in caveolin 3 molecular distribution and density at the sarcolemma of mdx mice by the fracture-label electron microscopic technique. At the sarcolemma of skeletal muscles from mdx mice, the densities of gold particles associated with caveolae, non-associated with caveolae and arranged circularly without caveolae were higher than those in control mice (P<0.01, P<0.01 and P<0.05 by two-tailed t-test), although in mdx mice, the overall arrangement of gold particles appeared to be irregular. These findings may reflect the active process of caveolar formation and the results of the disrupted protein-protein interaction in dystrophin-deficient muscle plasma membrane.
authors.affiliation: Department of Neurology, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-Ku, Yokohama 227-8501, Japan. seiji-shibuya@nifty.com
authors.forename: Seiji
authors.initials: S
authors.lastname: Shibuya
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Wakayama
authors.forename: Masahiko
authors.initials: M
authors.lastname: Inoue
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Oniki
authors.forename: Eiki
authors.initials: E
authors.lastname: Kominami
mesh_terms: D000818:Animals; D051244:Caveolin 3; D022461:Caveolins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry / Q000473:pathology; D016189:Dystrophin / Q000172:deficiency*; D051379:Mice; D018101:Mice, Inbred mdx; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000473:pathology*; D012508:Sarcolemma / Q000737:chemistry / Q000473:pathology
chemical_list: C495548:Cav3 protein, mouse; D051244:Caveolin 3; D022461:Caveolins; D016189:Dystrophin