title: Indocarbazostatin and indocarbazostatin B, novel inhibitors of NGF-induced neuronal differentiation in PC12 cells. I. Screening, taxonomy, fermentation and biological activities.
abstract: During the course of our screening for modulators of signal transduction of mammalian cells, we discovered two novel indolocarbazole antibiotics, indocarbazostatin and indocabazostatin B, from a culture broth of a Streptomyces sp. as inhibitors of NGF-induced neuronal differentiation in rat pheochromocytoma PC12 cells. Indocarbazostatin and indocarbazostatin B inhibited NGF-induced neurite outgrowth from PC12 cells at 6 nM and 24 nM, respectively, whereas K-252a inhibited at 200 nM under our assay conditions.
authors.affiliation: Biotechnology Research Center, Toyama Prefectural University, Kosugi, Japan.
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Matsuura
authors.forename: Norimasa
authors.initials: N
authors.lastname: Tamehiro
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Andoh
authors.forename: Akira
authors.initials: A
authors.lastname: Kawashima
authors.forename: Makoto
authors.initials: M
authors.lastname: Ubukata
mesh_terms: D000818:Animals; D002227:Carbazoles / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D003470:Culture Media; D005285:Fermentation; D006131:Growth Inhibitors / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D020932:Nerve Growth Factor / Q000037:antagonists & inhibitors*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D013302:Streptomyces / Q000145:classification / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002227:Carbazoles; D003470:Culture Media; D006131:Growth Inhibitors; D007211:Indoles; C402701:indocarbazostatin; C458887:indocarbazostatin B; D020932:Nerve Growth Factor