title: Bacteriologic evaluation of the effect of Nd:YAG laser irradiation in experimental infected root canals.
abstract: The aim of this study was to evaluate the efficacy of the Pumped Diodium-Nd:YAG laser in sterilizing contaminated root canals. After hand instrumentation, 30 teeth were inoculated with Actinomyces naeslundii CH-12 and 30 teeth with Pseudomonas aeruginosa ATCC 27853 and incubated for 24 h. The teeth were divided into three subgroups: subgroup A received no treatment; subgroup B was irradiated with laser (5 Hz for 15 s or 10 Hz for 15 s); and subgroup C was irrigated with 5.25% NaOCl. The number of viable bacteria in each group was evaluated by using the surface-spread plate technique. The results indicated an average of 34.0% decrease in colony-forming units for A. naeslundii CH-12 and 15.7% for P. aeruginosa ATCC 27853 with the 5 Hz/15 s laser treatment, and for the 10 Hz laser frequency, a decrease of the 77.4% for A. naeslundii CH-12 and 85.8% for P. aeruginosa ATCC 27853. No bacteria were detected in the canals treated with 5.25% NaOCl. The results show an antibacterial effect of the Pumped Diodium Nd:YAG laser, depending on the radiation frequency. However, 5.25% NaOCl was more effective than either laser application.
authors.affiliation: Department of Biomedical Sciences, School of Medicine, G. d'Annunzio University, Chieti, Italy.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Piccolomini
authors.forename: Camillo
authors.initials: C
authors.lastname: D'Arcangelo
authors.forename: Simonetta
authors.initials: S
authors.lastname: D'Ercole
authors.forename: Giovanni
authors.initials: G
authors.lastname: Catamo
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Schiaffino
authors.forename: Pietro
authors.initials: P
authors.lastname: De Fazio
mesh_terms: D000190:Actinomyces / Q000254:growth & development / Q000528:radiation effects*; D000538:Aluminum Silicates; D016009:Chi-Square Distribution; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004202:Disinfectants / Q000627:therapeutic use; D006801:Humans; D053685:Laser Therapy; D009354:Neodymium; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000528:radiation effects*; D012388:Root Canal Irrigants / Q000627:therapeutic use; D018915:Root Canal Preparation / Q000379:methods; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D013223:Statistics as Topic; D013242:Sterilization / Q000379:methods*; D015019:Yttrium
chemical_list: D000538:Aluminum Silicates; D004202:Disinfectants; D012388:Root Canal Irrigants; D009354:Neodymium; D015019:Yttrium; D012973:Sodium Hypochlorite