title: [Multidrug resistance in Klebsiella pneumoniae: multicenter study].
abstract: The extensive use of broad spectrum antibiotics, especially the third generation cephalosporins (C3G), was followed by the emergence of newer plasmid mediated betalactamases called extended spectrum betalactamases (ESBLs). To assess the impact of K. pneumoniae resistant to 3GC in Tunisia, this study was conducted in 3 teaching hospitals. A total of 1110 strains of K pneumoniae was collected. The antibiotics susceptibilities were tested by diffusion method using Mueller-Hinton agar. The quality control was regularly performed. I ESBLs producing solates were detected using the double-disc synergy test. Data analysis was done using the Whonet 4 software. 23.6% K. pneumoniae isolates showed phenotype pattern of ESBLs producers. The double-disc synergy test was positive in 75% of the cases. These isolates were recovered from hospitalized patients in different wards but mainly from pediatrics (23.6%), medicine (23.2%), surgery (22.9%), intensive care units (11%) and neonatology (11%). 54% were isolated from urines, 22% from blood cultures. These isolates remained susceptible to imipenem (100%) and most of them to cefoxitin (96.4%) but all had associated resistance to aminoglycosides, quinolones and trimethoprim-sulfamethoxazole. The prevalence of multidrug resistant K. pneumoniae is high. This resistance can be minimized by the implementation of infection control measures including handwashing and isolation procedures.
authors.affiliation: Laboratoire de Recherche Résistance aux antibiotiques, Faculté de Médecine 1006, Tunis.
authors.forename: Ilhem
authors.initials: I
authors.lastname: Boutiba-Ben Boubaker
authors.forename: Dorra
authors.initials: D
authors.lastname: Ben Salah
authors.forename: Makram
authors.initials: M
authors.lastname: Besbes
authors.forename: Faouzia
authors.initials: F
authors.lastname: Mahjoubi
authors.forename: Rafiaa
authors.initials: R
authors.lastname: Ghozzi
authors.forename: Saida
authors.initials: S
authors.lastname: Ben Redjeb
authors.forename: Assia
authors.initials: A
authors.lastname: Ben Hassen
authors.forename: Adnène
authors.initials: A
authors.lastname: Hammami
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D018432:Drug Resistance, Multiple; D005260:Female; D006784:Hospitals, Teaching / Q000706:statistics & numerical data; D006801:Humans; D015994:Incidence; D007223:Infant; D007231:Infant, Newborn; D017053:Infection Control / Q000379:methods; D007710:Klebsiella Infections / Q000188:drug therapy*; D007711:Klebsiella pneumoniae / Q000302:isolation & purification / Q000472:pathogenicity*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D015995:Prevalence; D012189:Retrospective Studies; D014416:Tunisia / Q000453:epidemiology
vernacular_title: Multiresistance aux antibiotiques de Klebsiella pneumoniae: etude multicentrique.