title: A cluster of candidate odorant receptors from the malaria vector mosquito, Anopheles gambiae.
abstract: Olfaction is critical to the host preference selection behavior of many disease-transmitting insects, including the mosquito Anopheles gambiae sensu stricto (hereafter A. gambiae), one of the major vectors for human malaria. In order to more fully understand the molecular biology of olfaction in this insect, we have previously identified several members member of a family of candidate odorant receptor proteins from A. gambiae (AgORs). Here we report the cloning and characterization of an additional AgOR gene, denoted as AgOr5, which shows significant similarity to putative odorant receptors in A. gambiae and Drosophila melanogaster and which is selectively expressed in olfactory organs. AgOr5 is tightly clustered within the A. gambiae genome to two other highly homologous candidate odorant receptors, suggesting that these genes are derived from a common ancestor. Analysis of the developmental expression within members of this AgOR gene cluster reveals considerable variation between these AgORs as compared to candidate odorant receptors from D. melanogaster.
authors.affiliation: Department of Biological Sciences, Program in Developmental Biology and Center for Molecular Neuroscience, Vanderbilt University, Nashville, TN 37235, USA.
authors.forename: A N
authors.initials: AN
authors.lastname: Fox
authors.forename: R J
authors.initials: RJ
authors.lastname: Pitts
authors.forename: L J
authors.initials: LJ
authors.lastname: Zwiebel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D005260:Female; D017344:Genes, Insect / Q000235:genetics; D007303:Insect Vectors / Q000235:genetics / Q000502:physiology*; D008288:Malaria / Q000635:transmission; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D018035:Receptors, Odorant / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D018035:Receptors, Odorant