Purification and characterization of benzoate:coenzyme A ligase from Clarkia breweri.
Benzoate:CoA ligase (BZL) was partially purified from flowers of the annual California plant Clarkia breweri. BZL catalyzes the formation of benzoyl-CoA and anthraniloyl-CoA, important intermediates for subsequent acyltransferase reactions in plant secondary metabolism. The native enzyme is active as a monomer with a molecular mass of approximately 59-64.5 kDa, and it has K(m) values of 45, 95, and 130 microM for benzoic acid, ATP, and CoA, respectively. BZL is most active in the pH range of 7.2-8.4, and its activity is strictly dependent on certain bivalent cations. BZL is an AMP-forming enzyme. Overall, its properties suggest that it is related to the family of CoA ligase enzymes that includes the plant enzyme 4-hydroxycinnamate:CoA ligase.
Beuerle
Till
T
Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 N. University Street, Ann Arbor, MI 48109-1048, USA.
Pichersky
Eran
E
D000249:Adenosine Monophosphate / Q000032:analysis / Q000096:biosynthesis; D000255:Adenosine Triphosphate / Q000378:metabolism; D019817:Benzoic Acid / Q000378:metabolism; D002413:Cations, Divalent / Q000737:chemistry / Q000494:pharmacology; D003065:Coenzyme A / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D029594:Onagraceae / Q000201:enzymology*; D018514:Plant Structures / Q000737:chemistry / Q000201:enzymology; D011189:Potassium Chloride / Q000737:chemistry / Q000494:pharmacology; D013379:Substrate Specificity; D013696:Temperature
D002413:Cations, Divalent; D000249:Adenosine Monophosphate; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D019817:Benzoic Acid; D003066:Coenzyme A Ligases; C055455:benzoate coenzyme A ligase; D003065:Coenzyme A