title: Cellular distribution of proteolytic enzymes in the skeletal muscle of sarcoid myopathy.
abstract: To clarify the mechanism of muscle fiber destruction in sarcoid myopathy, muscle biopsy specimens were examined from patients with sarcoid myopathy, polymyositis, or dermatomyositis. In sarcoid myopathy, noncaseating granulomatous lesions were located in the perimysium or endomysium or both. Little fiber atrophy, caused by mechanical compression of the granuloma, was seen, and there was no evidence of ischemia-induced changes (i.e., perifascicular atrophy) due to microangiopathy in muscles. Immunoreactivity for membrane-associated cytoskeletal proteins such as dystrophin and merosin was detected homogeneously along the surface of many small granulomas in intrafascicular lesions. These granulomas showed a characteristic phenotypic cellular distribution: CD68(+) and CD4(+) cells were present in the center, and some CD8(+) cells were found at the periphery, indicating typical sarcoid granuloma formation in each muscle fiber. Strong expression of proteases such as cathepsin B, calpain II and ubiquitin-proteasome was observed in macrophages and epithelioid cells but not in lymphocytes in granulomas within muscle fibers or those in the endomysium or perimysium. The expression intensity was stronger in premature-stage granulomas than in late-stage granulomas. Weak expression of these proteases was detected mainly in some muscle fibers invaded by epithelioid cells and macrophages and in a few atrophic or necrotic fibers adjacent to inflammatory foci but not in fibers of fascicles without granuloma formation or in fibers in perifascicular areas. Our results suggest that muscle fiber destruction in sarcoid myopathy is caused mainly by direct invasion of granulomatous inflammatory cells into muscle fibers during the process of granuloma formation rather than by mechanical compression or ischemia. Furthermore, the proteases derived from epithelioid cells and macrophages may play an important role in muscle fiber destruction.
authors.affiliation: Third Department of Internal Medicine, Oita Medical University, Idaigaoka 1-1, Hasama, Japan. kumagoro@oita-med.ac.jp
authors.forename: Toshihide
authors.initials: T
authors.lastname: Kumamoto
authors.forename: Keiko
authors.initials: K
authors.lastname: Yukishige
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ito
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nagao
authors.forename: Toshio
authors.initials: T
authors.lastname: Mori
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Ueyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsumura
authors.forename: Tomiyasu
authors.initials: T
authors.lastname: Tsuda
mesh_terms: D000328:Adult; D000368:Aged; D002154:Calpain / Q000032:analysis*; D002401:Cathepsin B / Q000032:analysis*; D003546:Cysteine Endopeptidases / Q000032:analysis*; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009097:Multienzyme Complexes / Q000032:analysis*; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000473:pathology; D009135:Muscular Diseases / Q000378:metabolism / Q000473:pathology*; D046988:Proteasome Endopeptidase Complex; D012507:Sarcoidosis / Q000378:metabolism / Q000473:pathology*; D025801:Ubiquitin / Q000032:analysis
chemical_list: D009097:Multienzyme Complexes; D025801:Ubiquitin; D002154:Calpain; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D046988:Proteasome Endopeptidase Complex