[Distribution of RNA polymerase II in nuclei of early murine embryos].
Nuclear distribution of unphosphorylated and phosphorylated forms of RNA polymerase II in two celled mouse embryos was studied using immunoelectron microscopy. RNA polymerase II was shown to be present in the karyoplasm of mouse embryo nuclei at the early two-celled stage, that is before activation of the embryonic genome. During this period, RNA polymerase II is diffusely localized in the nuclei being not associated with any nuclear domains. After embryonic genome activation, the pattern of the nuclear distribution of RNA polymerase II is changed. At the late two-celled stage, its prominent part is deposited in pronucleoli, as well as in interchromatin granule clusters and perichromatin fibrils. At this stage, unphosphorylated RNA polymerase II is mainly associated with perichromatin fibrils. The obtained data fit well with the notion of the coordinated distribution of RNA polymerase II and splicing factors, and of their association with the same nuclear domains.
Bogoliubova
I O
IO
Institute of Cytology RAS, St. Petersburg. ibogol@yahoo.com
Parfenov
V N
VN
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D004622:Embryo, Mammalian / Q000201:enzymology* / Q000648:ultrastructure; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D016253:Microscopy, Immunoelectron; D010766:Phosphorylation; D012319:RNA Polymerase II / Q000378:metabolism*
D012319:RNA Polymerase II
Raspredelenie RNK-polimerazy II v iadrakh rannikh myshinykh émbrionov.