title: Metabolic control analysis of anaerobic glycolysis in human hibernating myocardium replaces traditional concepts of flux control.
abstract: Myocardial hibernation represents an adaptation to sustained ischemia to maintain tissue vitality during severe supply-demand imbalance which is characterized by an increased glucose uptake. To elucidate this adaptive protective mechanism, the regulation of anaerobic glycolysis was investigated using human biopsies. In hibernating myocardium showing an increase in anaerobic glycolytic flux metabolizing exogenous glucose, the adjustment of flux through this pathway was analyzed by flux:metabolite co-responses. By this means, a previously unknown pattern of regulation using multisite modulation was found which largely differs from traditional concepts of metabolic control of the Embden-Meyerhof pathway in normal and diseased myocardium.
authors.affiliation: Department of Cardiology, University of Heidelberg, Heidelberg, Germany. achim_vogt@med.uni-heidelberg.de
authors.forename: Achim M
authors.initials: AM
authors.lastname: Vogt
authors.forename: Holger
authors.initials: H
authors.lastname: Nef
authors.forename: Jutta
authors.initials: J
authors.lastname: Schaper
authors.forename: Mark
authors.initials: M
authors.lastname: Poolman
authors.forename: David A
authors.initials: DA
authors.lastname: Fell
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kübler
authors.forename: Albrecht
authors.initials: A
authors.lastname: Elsässer
mesh_terms: D000693:Anaerobiosis / Q000502:physiology*; D001706:Biopsy; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism*; D006019:Glycolysis / Q000502:physiology*; D006801:Humans; D017682:Myocardial Stunning / Q000097:blood / Q000378:metabolism*; D010477:Perfusion
chemical_list: D006003:Glycogen; D005947:Glucose