Generation and characterization of antibodies to sulfated glucuronyl glycolipids in Lewis rats.
Antibodies to sulfated glucuronyl glycolipids (SGGLs) have been reported in sera of patients with peripheral neuropathies including patients with IgM gammopathy. However, the role of anti-SGGL antibodies in the pathogenesis of neuropathy remains unclear. In order to study the role of antibodies to SGGLs in the pathogenesis of neuropathy, Lewis female rats were injected with purified SGPG mixed with keyhole limpet hemocyanin (KLH) and emulsified with equal amount of complete Freund's adjuvant. High titer anti-SGPG antibodies were detected by ELISA in sera of all rats inoculated with SGPG. All anti-SGPG antibodies cross-reacted with human myelin-associated glycoprotein (MAG). None of the sensitized rats exhibited clinical signs of neuropathy. Histological examination showed that there was no demyelination or axonal damage in peripheral nerves. Our data demonstrate that SGPG is a highly immunogenic glycolipid but high titer antibodies against it do not produce an experimental autoimmune neuropathy in Lewis rats.
Ilyas
Amjad A
AA
Department of Neurosciences, UMDNJ-New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA. ilyasaa@umdnj.edu
Chen
Zei Wei
ZW
Prineas
John W
JW
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D003429:Cross Reactions; D004285:Dogs; D005260:Female; D006017:Glycolipids / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D018992:Myelin-Associated Glycoprotein / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D012584:Sciatic Nerve / Q000737:chemistry / Q000276:immunology / Q000473:pathology*; D020426:Sciatic Neuropathy / Q000276:immunology* / Q000473:pathology
D001323:Autoantibodies; D006017:Glycolipids; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D018992:Myelin-Associated Glycoprotein; C011117:sulfoglycolipids