Regulatory interaction of phosducin-like protein with the cytosolic chaperonin complex.
Phosducin and phosducin-like protein (PhLP) bind G protein betagamma subunits and regulate their activity. This report describes a previously uncharacterized binding partner unique to PhLP that was discovered by coimmunoprecipitation coupled with mass spectrometric identification. Chaperonin containing tailless complex polypeptide 1 (CCT), a cytosolic chaperone responsible for the folding of many cellular proteins, binds PhLP with a stoichiometry of one PhLP per CCT complex. Unlike protein-folding substrates of CCT, which interact only in their nonnative conformations, PhLP binds in its native state. Native PhLP competes directly for binding of protein substrates of CCT and thereby inhibits CCT activity. Overexpression of PhLP inhibited the ability of CCT to fold newly synthesized beta-actin by 80%. These results suggest that the interaction between PhLP and CCT may be a means to regulate CCT-dependent protein folding or alternatively, to control the availability of PhLP to modulate G protein signaling.
McLaughlin
Joseph N
JN
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
Thulin
Craig D
CD
Hart
Sarah J
SJ
Resing
Katheryn A
KA
Ahn
Natalie G
NG
Willardson
Barry M
BM
D000199:Actins / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D016466:CHO Cells; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D018833:Chaperonins / Q000378:metabolism*; D003001:Cloning, Molecular; D006224:Cricetinae; D003600:Cytosol / Q000378:metabolism; D018076:DNA, Complementary; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D013058:Mass Spectrometry; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D017510:Protein Folding; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014162:Transfection
D000199:Actins; D002352:Carrier Proteins; D018076:DNA, Complementary; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; C498320:Pdcl protein, rat; D011994:Recombinant Proteins; D018833:Chaperonins