title: Fibrinogen in rat gastrointestinal lymph before, during and after intraduodenal administration of emulsified triglyceride: fibrinogen bound to chylomicrons in gastrointestinal lymph is functional.
abstract: Samples of gastrointestinal lymph were collected from fasted, male, Sprague-Dawley rats before, during and after intraduodenal administration of either a phospholipid-stabilized, triglyceride-rich emulsion or the dextrose-saline diluent of the emulsion. In lipid-treated rats, the triglyceride, total protein, and functional fibrinogen contents of lymph increased significantly during the 4 h of continuous lipid infusion, with all analytes returning to near baseline values by 20 h later. Levels of the same analytes changed little, if at all, in control animals. As assessed using immunoblotting, chylomicrons in gastrointestinal lymph are coated with fibrinogen. Fibrinogen-coated chylomicrons readily incorporate into solution phase clots and, in the presence of thrombin, adhere in heparin-preventable fashion to each other and to other fibrinogen-coated surfaces. Taken together, these data indicate that lipid feeding creates in gastrointestinal lymph a condition that is conducive temporally to the physical association of fibrinogen with newly ingested lipids before they reach the circulatory system.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45267-0529, USA.
authors.forename: Ashley P
authors.initials: AP
authors.lastname: DeAnglis
authors.forename: Charity M
authors.initials: CM
authors.lastname: Einhaus
authors.forename: Amy D
authors.initials: AD
authors.lastname: Sombun
authors.forename: Looi C
authors.initials: LC
authors.lastname: Ee
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Retzinger
mesh_terms: D000268:Adhesiveness; D000818:Animals; D002914:Chylomicrons / Q000378:metabolism; D004064:Digestive System; D004655:Emulsions / Q000008:administration & dosage; D005340:Fibrinogen / Q000187:drug effects / Q000378:metabolism*; D008196:Lymph / Q000737:chemistry*; D008297:Male; D019518:Postprandial Period; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D013917:Thrombin / Q000494:pharmacology; D014280:Triglycerides / Q000008:administration & dosage*
chemical_list: D002914:Chylomicrons; D004655:Emulsions; D014280:Triglycerides; D005340:Fibrinogen; D013917:Thrombin