The lon gene, encoding an ATP-dependent protease, is a novel member of the HAIR/HspR stress-response regulon in actinomycetes.
Members of a family of ATP-dependent proteases related to Lon from Escherichia coli are present in most prokaryotes and eukaryotes. These proteases are generally reported to be heat induced, and various regulatory systems have been described. The authors cloned and disrupted the lon gene and studied the regulation of its expression in Streptomyces lividans. lon is negatively regulated by the HspR/HAIR repressor/operator system, suggesting that Lon is produced concomitantly with the other members of this regulon, DnaK and ClpB. The lon mutant grew more slowly than the wild-type and spore germination was impaired at high temperature. Nevertheless its cell cycle was not greatly affected and it sporulated normally.
Sobczyk
André
A
VIVALIS SA, CHU de Nantes - Hôtel-Dieu, Place A. Ricordeau, 44093 Nantes Cedex 1, France2.|Unité de Biochimie Microbienne, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France1.
Bellier
Audrey
A
Unité de Biochimie Microbienne, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France1.
Viala
Julie
J
Unité de Biochimie Microbienne, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France1.
Mazodier
Philippe
P
Unité de Biochimie Microbienne, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France1.
D000190:Actinomyces / Q000235:genetics*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D001426:Bacterial Proteins; D015153:Blotting, Western; D003001:Cloning, Molecular; D024202:Electrophoretic Mobility Shift Assay; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010447:Peptide Hydrolases / Q000235:genetics* / Q000378:metabolism; D018085:Regulon / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013171:Spores, Bacterial; D013302:Streptomyces / Q000235:genetics; D024363:Transcription Initiation Site
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; C108444:HspR protein, bacteria; D012097:Repressor Proteins; D000255:Adenosine Triphosphate; D010447:Peptide Hydrolases