Leptin induces IL-1 receptor antagonist expression in the brain.
The ob gene product, leptin, is known to be an important circulating signal for regulation of food intake and body weight. We report here that peripherally applied leptin increased interleukin-1 receptor antagonist (IL-1ra) transcripts in the hypothalamus. Interestingly, we observed leptin-induced increase in IL-1ra transcripts not only in the hypothalamus but also in other brain regions such as the hippocampus, the cortex, the cerebellum, and the brain stem. Moreover, leptin applied to the db/db mice, which lack functional Ob-Rb receptor, increased IL-1ra mRNA levels in the hyothalamus to a similar extent as in normal mice. These results indicate that the leptin-induced IL-1ra expression in the brain may be mediated through STAT3 independent mechanisms.
Hosoi
Toru
T
Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
Okuma
Yasunobu
Y
Nomura
Yasuyuki
Y
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D053590:Interleukin 1 Receptor Antagonist Protein; D020738:Leptin / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000235:genetics; D015534:Trans-Activators / Q000378:metabolism
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D020738:Leptin; D020033:Protein Isoforms; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D050796:STAT3 Transcription Factor; D012795:Sialoglycoproteins; C494087:Stat3 protein, mouse; D015534:Trans-Activators; C515447:leptin receptor, mouse