Inhibitory effect of olopatadine on antigen-induced eosinophil infiltration and the LFA-1 and Mac-1 expression in eosinophils.
The inhibitory effect of olopatadine, a new antiallergic drug, on antigen-induced eosinophil infiltration and its mechanisms were examined using the local sensitized rat allergic rhinitis model and isolated IL-5-stimulated rat peritoneal eosinophils. Olopatadine dose-dependently inhibited antigen-induced eosinophil infiltration in the nasal mucosa. Olopatadine dose-dependently repressed the IL-5-induced expressions of CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1) on rat peritoneal eosinophils. However, olopatadine had no effect on IL-5-induced CD49d/CD29 (VLA-4) expression. These results suggest that olopatadine may inhibit antigen-induced eosinophil infiltration through repression of LFA-1 and Mac-1 expression.
Fukuishi
Nobuyuki
N
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
Matsuhisa
Motoko
M
Shimono
Takeshi
T
Murata
Nobutoshi
N
Iwanaga
Mari
M
Sagara
Hidenori
H
Matsui
Nobuaki
N
Akagi
Masaaki
M
D000284:Administration, Oral; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D003990:Dibenzoxepins / Q000494:pharmacology*; D004804:Eosinophils / Q000187:drug effects*; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D066298:In Vitro Techniques; D039041:Integrin alpha4beta1 / Q000096:biosynthesis; D016169:Lymphocyte Function-Associated Antigen-1 / Q000187:drug effects*; D016177:Macrophage-1 Antigen / Q000096:biosynthesis*; D008297:Male; D009297:Nasal Mucosa / Q000473:pathology; D000069605:Olopatadine Hydrochloride; D010793:Phthalazines / Q000494:pharmacology; D051381:Rats; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy / Q000473:pathology
D018926:Anti-Allergic Agents; D003990:Dibenzoxepins; D006634:Histamine H1 Antagonists; D039041:Integrin alpha4beta1; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D010793:Phthalazines; D000069605:Olopatadine Hydrochloride; C020976:azelastine