Detection and purification of instructive extracellular matrix components with monoclonal antibody technologies.
Historically, Mabs have been one of the most productive and reliable methods for the identification of adhesion receptors and adhesive ECM ligands. In large part, this is because Mabs can identify the function of the adhesion components within the context of the complex ECM or the cell surface. There are now many isoforms of laminin, collagen, and other ECM components that have been identified by molecular and Mab approaches. It is not clear when and where these isoforms are expressed at the protein level, nor what unique functions each ECM isoform may serve within the context of tissue. Undoubtedly, specific in vitro assays in combination with specific Mabs will help illuminate the instructive roles of ECM components for reporter cells within in vitro models and tissue. Delineation of cell responses to the instructive ECM will require additional high-resolution technologies including DNA microarrays and targeted disruption of ECM components.
Gil
Susana G
SG
Fred Hutchinson Cancer Research Institute, Seattle, Washington 98109, USA.
Sigle
Randy O
RO
Carter
William G
WG
D000818:Animals; D000911:Antibodies, Monoclonal; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D000078404:Epidermal Cells; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007158:Immunologic Techniques; D039161:Integrin alpha6beta4 / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
D000911:Antibodies, Monoclonal; D016326:Extracellular Matrix Proteins; D039161:Integrin alpha6beta4; D007797:Laminin; D011956:Receptors, Cell Surface; D012710:Serum Albumin, Bovine