title: Dietary restriction reduces atherosclerosis and oxidative stress in the aorta of apolipoprotein E-deficient mice.
abstract: Dietary restriction (DR) has been shown to inhibit almost all the age-related diseases, e.g. cardiomyopathy and cancers, in rodents. However, there is little information for the effect of DR on atherosclerosis. In the present study, we examined the effect of DR on the development of atherosclerosis in mice homozygous knockout for apolipoprotein E gene (ApoE(-/-)). The ApoE(-/-) mice were fed either ad libitum (AL) or 60% of the diet consumed by the mice fed AL. Atherosclerotic lesions in the proximal aorta of these mice were measured. Our results showed that ApoE(-/-) mice fed the calorie-restricted diet had smaller and relatively early stages of atherosclerotic lesions (e.g. foam cells and free lipids) when compared to ApoE(-/-) mice fed AL, who developed more advanced lesions (e.g. fibrous caps and acellular areas). In addition, ApoE(-/-) mice fed the calorie-restricted diet showed a significant decrease in the level of lipid hydroperoxides and the production of superoxide and hydrogen peroxide in the aorta as compared to ApoE(-/-) mice fed AL. These observations suggest that reduction of oxidative stress in the arterial wall may contribute to the anti-atherogenic effect of DR in ApoE(-/-) mice.
authors.affiliation: Department of Physiology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA. zguo@mmx.edu
authors.forename: ZhongMao
authors.initials: Z
authors.lastname: Guo
authors.forename: Felicia
authors.initials: F
authors.lastname: Mitchell-Raymundo
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ikeno
authors.forename: James
authors.initials: J
authors.lastname: Nelson
authors.forename: Vivian
authors.initials: V
authors.lastname: Diaz
authors.forename: Arlan
authors.initials: A
authors.lastname: Richardson
authors.forename: Robert
authors.initials: R
authors.lastname: Reddick
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism* / Q000473:pathology; D001057:Apolipoproteins E / Q000235:genetics / Q000378:metabolism*; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology; D002784:Cholesterol / Q000097:blood; D004032:Diet; D004195:Disease Models, Animal; D002149:Energy Intake; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D018384:Oxidative Stress; D013481:Superoxides / Q000378:metabolism
chemical_list: D001057:Apolipoproteins E; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D013481:Superoxides; D002784:Cholesterol; D006861:Hydrogen Peroxide