title: Identification of G protein-coupled receptor genes from the human genome sequence.
abstract: We have identified novel G protein-coupled receptors (GPCRs) with no introns in the coding region from the human genome sequence: 322 olfactory receptors; 22 taste receptors; 128 registered GPCRs for endogenous ligands; 50 novel GPCR candidates homologous to registered GPCRs for endogenous ligands; and 59 novel GPCR candidates not homologous to registered GPCRs. The total number of GPCRs with and without introns in the human genome was estimated to be approximately 950, of which 500 are odorant or taste receptors and 450 are receptors for endogenous ligands.
authors.affiliation: Department of Neurochemistry, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Takeda
authors.forename: Shiro
authors.initials: S
authors.lastname: Kadowaki
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Haga
authors.forename: Hirotomo
authors.initials: H
authors.lastname: Takaesu
authors.forename: Shigeki
authors.initials: S
authors.lastname: Mitaku
mesh_terms: D000328:Adult; D004247:DNA / Q000737:chemistry / Q000235:genetics; D030561:Databases, Nucleic Acid; D019204:GTP-Binding Proteins / Q000378:metabolism; D020869:Gene Expression Profiling; D015894:Genome, Human; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009832:Olfactory Nerve / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: C519854:GPR119 protein, human; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D004247:DNA; D019204:GTP-Binding Proteins