Pasteurella multocida exbB, exbD and tonB genes are physically linked but independently transcribed.
The exbB, exbD and tonB genes of the Pasteurella multocida animal pathogen have been cloned by complementation of an Escherichia coli tonB mutant. Despite these three genes being physically linked, RT-PCR analysis, lacZ transcriptional fusions and construction of insertional mutants have demonstrated that they do not constitute an operon, but rather are transcribed independently from each other. Furthermore, expression of these three genes is under iron control as revealed by lacZ fusions and Fur titration assay analysis. Moreover, each of these three genes is necessary for the virulence of P. multocida cells and all of them contribute equally to the infectious process of this microorganism.
Bosch
Montserrat
M
Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
Garrido
Elena
E
Llagostera
Montserrat
M
Pérez de Rozas
Ana M
AM
Badiola
Ignacio
I
Barbé
Jordi
J
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D023061:Gene Order / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics* / Q000502:physiology; D005816:Genetic Complementation Test; D008040:Genetic Linkage / Q000235:genetics*; D007501:Iron / Q000494:pharmacology; D007928:Lethal Dose 50; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology; D005810:Multigene Family / Q000235:genetics; D009154:Mutation; D016979:Pasteurella multocida / Q000235:genetics* / Q000472:pathogenicity; D011401:Promoter Regions, Genetic / Q000235:genetics; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics*; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D012329:RNA, Bacterial; C057616:tonB protein, Bacteria; C489181:tonB protein, E coli; C060790:exbD protein, E coli; D007501:Iron