title: Assessment of chromosomal losses and gains in hepatocellular carcinoma.
abstract: We examined the chromosomal changes of 22 hepatocellular carcinomas (HCCs) by comparative genomic hybridization (CGH) analysis and compared the results with that of allelotype by polymerase chain reaction based loss of heterozygosity (PCR-LOH) analysis. By CGH analysis, frequent chromosomal losses were noted in the chromosomal region of 4q (59%), 8p (77%), and 16q (50%), whereas gains were noted in 1q (86%) and 8q (77%). All of these chromosomal arms were revealed to have frequent allelic imbalances by PCR-LOH analysis, however, 9% of chromosomal aberrations were detected only by CGH analysis and 2% were detected only by PCR-LOH analysis. Our results suggest that CGH analysis gives more precise results for the screening of chromosomal aberrations in HCCs than that of PCR-LOH analysis with randomly selected microsatellite markers.
authors.affiliation: Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Joon
authors.initials: J
authors.lastname: Chang
authors.forename: Nam-Gyun
authors.initials: NG
authors.lastname: Kim
authors.forename: Zhe
authors.initials: Z
authors.lastname: Piao
authors.forename: Chanil
authors.initials: C
authors.lastname: Park
authors.forename: Kang-Sik
authors.initials: KS
authors.lastname: Park
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Paik
authors.forename: Woo-Jung
authors.initials: WJ
authors.lastname: Lee
authors.forename: Byong Ro
authors.initials: BR
authors.lastname: Kim
authors.forename: Hoguen
authors.initials: H
authors.lastname: Kim
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D020440:Gene Duplication; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity; D016133:Polymerase Chain Reaction / Q000379:methods