title: Distribution of bovine lymphocyte antigen (BoLA-DRB3) alleles in Brazilian dairy Gir cattle (Bos indicus).
abstract: Brazilian dairy Gir (Bos indicus) cattle are a tropical, well-adapted breed, for which no information on the major histocompatibility complex (MHC) is presently available. The second exon of the bovine lymphocyte antigen (BoLA-DRB3) was amplified by polymerase chain reaction (PCR) of DNA samples from 28 Brazilian dairy Gir cows. Two experimental genotyping approaches were used: direct sequencing of PCR gene products (PCR-DS) and sequencing of cloned PCR fragments (S-CLO). Results demonstrate the viability of both typing approaches. PCR-DS allowed typing of 39% of the animals while the remainder were genotyped by S-CLO. Seventeen BoLA-DRB3 alleles were assigned, including some that were only recently described for zebu cattle. Allelic frequencies ranged from 0.02 to 0.18. The most frequent alleles were *3601 (frequency = 0.18), *2201 (0.14) and *2101 (0.11).
authors.affiliation: Laboratory of Animal Biotechnology, Department of Animal Production, ESALQ, University of São Paulo, SP, Brazil.
authors.forename: A F
authors.initials: AF
authors.lastname: da Mota
authors.forename: J E
authors.initials: JE
authors.lastname: Gabriel
authors.forename: M L
authors.initials: ML
authors.lastname: Martinez
authors.forename: L L
authors.initials: LL
authors.lastname: Coutinho
mesh_terms: D000483:Alleles; D000818:Animals; D001938:Brazil; D002417:Cattle / Q000235:genetics*; D002845:Chromatography; D004587:Electrophoresis, Agar Gel; D005787:Gene Frequency; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: C423282:BoLA-DRB3 antigen; D000949:Histocompatibility Antigens Class II