Constitutive activation of Stat5a by retrovirus integration in early pre-B lymphomas of SL/Kh strain mice.
We found that the second intron of Stat5a was one of the common integration sites of the endogenous ecotropic murine leukemia virus, i.e., SL/Kh virus integration-1 (Svi1), in early pre-B lymphomas in SL/Kh mice. The high expression of STAT5A induced by Svi1 integration and activation accelerated the transcription of its target genes such as c-Myc. Transfection of the constitutively active Stat5a mutant cDNA, but not of the wild-type cDNA, to the bone marrow cells induced colony formation of pre-B cells in a methylcellulose medium and escaped from dependence on IL-7. Such growth depended on a genetic factor in the SL/Kh strain. Consitutively high expression of Stat5a either by retrovirus integration or transfection of active mutant cDNA can be lymphomagenic to early pre-B cells in collaboration with a certain genetic background factor of mice.
Tsuruyama
Tatsuaki
T
Department of Pathology and Biology of Diseases, Kyoto University Graduate School of Medicine, 1 Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
Nakamura
Takuro
T
Jin
Guang
G
Ozeki
Munetaka
M
Yamada
Yoshihiro
Y
Hiai
Hiroshi
H
D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology / Q000473:pathology; D002455:Cell Division; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D016259:Genes, myc; D007438:Introns; D009052:Leukemia Virus, Murine / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000821:virology; D051379:Mice; D008815:Mice, Inbred Strains; D008894:Milk Proteins; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050799:STAT5 Transcription Factor; D015534:Trans-Activators / Q000235:genetics*; D014162:Transfection; D016662:Virus Integration
D020413:3' Untranslated Regions; D017931:DNA Primers; D004268:DNA-Binding Proteins; D008894:Milk Proteins; D050799:STAT5 Transcription Factor; C494093:Stat5a protein, mouse; D015534:Trans-Activators