[Stereologic study of the cerebellar Purkinje cells submitted to alcoholic intoxication in Wistar rats].
BACKGROUND
to analyze the effect of the alcohol on the cells of Purkinje.


METHODS
Wistar rats received alcoholic solutions orally in different concentrations 4%, 12% and 24%. The animals were sacrificed with 4, 8 and 12 weeks and the cerebella were randomly cut and embedded in paraffin. Sections of 6 micrometer (H&E) were stereologically analyzed.


RESULTS
The differences among the density for area and density of surface of the cells of Purkinje of all of the experimental groups (E) and the respective controls (C) were significant. With 12 weeks the cell of Purkinje volume density decreased among the groups C and E in the concentrations of 4% and 12%, but not for the concentration of 24%, probably due to smaller liquid ingestion by the animals.


CONCLUSIONS
The alcohol has toxic effect on the Purkinje cellular body in the three studied concentrations from 4 weeks.
Apfel
Mara Ibis Rodrigues
MI
Laboratório de Histologia e Embriologia, Departamento de Histologia e Embriologia, Instituto de Biologia Roberto Alcântara Gomes, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
Esberard
Charles Alfred
CA
Rodrigues
Fabrício Kafury Pereira
FK
Bahamad Júnior
Fayez Mohamad
FM
Sillero
Rafael Oliveira
RO
D000435:Alcoholic Intoxication / Q000503:physiopathology*; D000818:Animals; D002492:Central Nervous System Depressants / Q000633:toxicity*; D000431:Ethanol / Q000633:toxicity*; D008297:Male; D011689:Purkinje Cells / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D002492:Central Nervous System Depressants; D000431:Ethanol
Estudo estereológico das células de Purkinje cerebelares submetidas à intoxicação alcoólica em ratos Wistar.