Protein N-methyltransferase assays in the study of gene transcription.
Protein N-methylation has recently emerged as a major mechanism involved in regulating gene transcription and epigenetic inheritance. Transcriptional coactivators and corepressors have both been shown to associate with protein N-methyltransferases. These enzymes methylate arginine or lysine residues within histones as well as other proteins, such as transcription factors. Here, we discuss the recent advances in our understanding of protein methylation and its involvement in gene expression. Protocols are presented for studying transcriptionally relevant protein methylation.
Schneider
Robert
R
Wellcome/CRC UK Institute and Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK. ab149@cam.ac.uk
Bannister
Andrew J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000378:metabolism; D004330:Drosophila; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D006657:Histones; D010455:Peptides; D011993:Recombinant Fusion Proteins; D001120:Arginine; D008780:Methyltransferases; D008239:Lysine