Effect of Cassia auriculata leaf extract on lipids in rats with alcoholic liver injury.
We studied the effect of administering Cassia auriculata leaf extract to rats with experimentally induced liver damage. Hepatotoxicity was induced by administering 9.875 g/kg bodyweight ethanol for 30 days by intragastric intubation. C. auriculata leaf extract was administered at a dose of 250 mg/kg bodyweight daily in one group and 500 mg/kg bodyweight daily in another group of alcohol-treated rats. All rats were fed with standard pellets. The control rats were also given isocaloric glucose solution. The average bodyweight gain was significantly lower in alcohol-treated rats, but improved on supplementation with C. auriculata leaf extract. Alcohol supplementation significantly elevated the cholesterol, phospholipid and triglyceride concentration in the liver, brain, kidney and intestine, as compared with those of the normal control rats. Treatment with C. auriculata leaf extract and alcohol significantly lowered the tissue lipid levels to almost normal levels. Microscopic examination of alcohol-treated rat liver showed inflammatory cell infiltrates and fatty changes, which were reversed on treatment with C. auriculata leaf extract. Similarly, alcohol-treated rat brain demonstrated spongiosis, which was markedly reduced on treatment with C. auriculata. In conclusion, this study shows that treatment with C. auriculata leaf extract has a lipid-lowering effect in rats with experimentally induced, alcohol-related liver damage. This is associated with a reversal of steatosis in the liver and of spongiosis in the brain. The mechanism of C. auriculata leaf extract lipid-lowering potential is unclear.
Kumar
Rajagopal Senthil
RS
Department of Biochemistry, Annamalai University, Annamalainagar, Tamilnadu, India.
Ponmozhi
Manickam
M
Viswanathan
Periyasamy
P
Nalini
Namasivayam
N
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001921:Brain / Q000187:drug effects; D002366:Cassia; D004195:Disease Models, Animal; D008055:Lipids / Q000097:blood*; D008099:Liver / Q000187:drug effects; D008108:Liver Diseases, Alcoholic / Q000097:blood* / Q000188:drug therapy*; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar; D015430:Weight Gain / Q000187:drug effects
D008055:Lipids; D010936:Plant Extracts; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase