title: The role of mast cells in the development of 2, 4, 6-trinitrobenzene sulfonic acid-induced colitis in rats.
abstract: BACKGROUND
  The role of mast cells in Crohn disease (CD) remains to be established. The aim of this study was to elucidate this in the development of CD-like colitis in rats by the use of mast-cell-deficient Ws/Ws and their control W+/W+ rats.
  
  
  METHODS
  CD-like colitis was induced in both groups by an enema of 10 mg of 2,4, 6-trinitrobenzene sulfonic acid (TNBS) in 50% ethanol. Colonic damage, adhesion and colonic weight were measured at 7 and 14 days after the TNBS/ethanol enema. Rat mast cell protease-2 (RMCP-2) in the colonic tissue was also measured at 7 days after the enema.
  
  
  RESULTS
  There was no significant difference between W+/W+ and Ws/Ws rats in terms of colonic damage, adhesion or colonic weight. The tissue content of RMCP-2 in Ws/Ws rats treated with either saline or TNBS/ethanol was only maintained at a much lower level than that in W+/W+ rats with the corresponding treatment.
  
  
  CONCLUSIONS
  These results demonstrate that mast cells are not essential in the development of 2, 4, 6-trinitrobenzene sulfonic acid-induced colitis in rats.
authors.affiliation: Pharmacological Research Dept, Teijin Institute for Bio-Medical Research, Asahigaoka, Hino, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumoto
authors.forename: H
authors.initials: H
authors.lastname: Kasai
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
authors.forename: N
authors.initials: N
authors.lastname: Hase
authors.forename: H
authors.initials: H
authors.lastname: Kaneko
authors.forename: I
authors.initials: I
authors.lastname: Hamamura
authors.forename: Y
authors.initials: Y
authors.lastname: Aoki
authors.forename: M
authors.initials: M
authors.lastname: Ota
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Katsuura
authors.forename: T
authors.initials: T
authors.lastname: Kamimura
authors.forename: K
authors.initials: K
authors.lastname: Komoriya
mesh_terms: D000818:Animals; D053818:Chymases; D003092:Colitis / Q000139:chemically induced / Q000276:immunology; D003106:Colon / Q000378:metabolism; D003424:Crohn Disease / Q000139:chemically induced* / Q000276:immunology*; D008297:Male; D008407:Mast Cells / Q000276:immunology* / Q000502:physiology; D023421:Models, Animal; D051381:Rats; D012697:Serine Endopeptidases / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*
chemical_list: D014302:Trinitrobenzenesulfonic Acid; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases