Modulation of insulin-like growth factor-I production of cultured retinal vascular endothelial cells by oxygen, glucose and growth hormone.
OBJECTIVE
To evaluate the interaction of oxygen, glucose, and growth hormone (GH) on insulin-like growth factor-I (IGF-I) production of cultured bovine retinal vascular endothelial cells.


METHODS
Confluent cultures of bovine retinal vascular endothelial cells were incubated under 3% or 20% oxygen in Dulbecco's modified Eagle's medium (containing 1 g/L or 4.5 g/L glucose), with or without the addition of 10 ng/mL GH. After incubation times of 0, 24, 48, and 72 hours, IGF-I was measured in the supernatant, and cells were counted.


RESULTS
Highest levels of IGF-I were reached after 72 hours with 4.5 g/L glucose and 10 ng/mL GH under 3% O(2). All IGF-I levels found in 3% O(2) samples were significantly higher than those found in 20% O(2) samples. No statistical significance was found regarding glucose concentration or GH supplement.


CONCLUSIONS
Oxygen turned out to be the sole modulating factor for IGF-I production of cultured retinal vascular endothelial cells.
Eter
Nicole
N
Department of Ophthalmology, Bonn University Medical Center, Bonn, Germany.
Sahm
Monika
M
Klingmüller
Dietrich
D
Spitznas
Manfred
M
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004338:Drug Combinations; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D013006:Growth Hormone / Q000187:drug effects*; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D010100:Oxygen / Q000494:pharmacology*; D012171:Retinal Vessels / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D004338:Drug Combinations; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D005947:Glucose; D010100:Oxygen