title: Rare failures in the amelogenin sex test.
abstract: Determination of sex using the amelogenin sex test is well established in the forensic field especially for casework and DNA databasing purposes. The sex test is part of commercially available PCR kits. Among 29,432 phenotypic male individuals stored in the Austrian National DNA database, 6 individuals were found to lack the amelogenin Y-specific PCR product which was confirmed using alternative amelogenin primers. The amplification of eight Y-chromosomal STR markers resulted in full profiles in five out of the six samples, one sample failed to amplify Y-STRs at all. The amplification of a fragment of the SRY gene gave positive results in all six samples, confirming the male phenotype of the individuals. The observed failure rate of the amelogenin sex test was 0.018% in this study.
authors.affiliation: Institute of Legal Medicine, Innsbruck, Austria. martin.steinlechner@uibk.ac.at
authors.forename: M
authors.initials: M
authors.lastname: Steinlechner
authors.forename: B
authors.initials: B
authors.lastname: Berger
authors.forename: H
authors.initials: H
authors.lastname: Niederstätter
authors.forename: W
authors.initials: W
authors.lastname: Parson
mesh_terms: D053523:Amelogenin; D001317:Austria; D030561:Databases, Nucleic Acid; D003746:Dental Enamel Proteins / Q000235:genetics*; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012732:Sex Determination Analysis; D014998:Y Chromosome
chemical_list: D053523:Amelogenin; D003746:Dental Enamel Proteins