Combinatorial variation in coding and promoter sequences of genes at the Tri locus in Pisum sativum accounts for variation in trypsin inhibitor activity in seeds.
Cultivars of Pisum sativum that differ with respect to the quantitative expression of trypsin/chymotrypsin inhibitor proteins in seeds have been examined in terms of the structure of the corresponding genes. The patterns of divergence in the promoter and coding sequences are described, and the divergence among these exploited for the development of facile DNA-based assays to distinguish genotypes. Quantitative effects on gene expression may be attributed to the overall gene complement and to particular promoter/coding sequence combinations, as well as to the existence of distinct active-site variants that ultimately influence protein activity. Electronic supplementary material to this paper can be obtained by using the Springer LINK server located at http://dx.doi.org/10.1007/s00438-002-0667-4.
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Laboratoire des Légumineuses, INRA-URGAP Dijon, Domaine d'Epoisses, BP 86510, 21065 Dijon, France.
Aubert
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Duc
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Welham
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Domoney
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D005819:Genetic Markers; D014644:Genetic Variation; D018532:Pisum sativum / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000235:genetics* / Q000378:metabolism
D005819:Genetic Markers; D014361:Trypsin Inhibitors; D014357:Trypsin