title: Eicosapentaenoic acid inhibits CSF-induced human monocyte survival and maturation into macrophage through the stimulation of H2O2 production.
abstract: Human studies suggest a beneficial effect of eicosapentaenoic acid (EPA)-supplemented diets on atherosclerotic and atherothrombotic disorders as well as autoimmune and inflammatory diseases and tumors. The effects of EPA on human monocyte survival and maturation into macrophage are not yet known. We studied the effects of EPA on the survival and development into macrophage of human monocyte treated with colony-stimulating factor (CSF). We have found that EPA induces cell death of the monocyte via apoptosis, even in the presence of M-CSF or GM-CSF, and inhibits differentiation from the monocyte to macrophage by inducing H2O2 production. In contrast to the effect of EPA on monocytes, EPA did not induce cell death of monocyte-derived macrophages. Such an apoptosis inducing effect on monocytes by EPA may contribute to the efficacy of EPA in atherosclerosis and autoimmune diseases.
authors.affiliation: Division of Clinical Nutrition, National Institute of Health and Nutrition, Tokyo, Japan.
authors.forename: Sachiyo
authors.initials: S
authors.lastname: Terada
authors.forename: Mari
authors.initials: M
authors.lastname: Takizawa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Osamu
authors.initials: O
authors.lastname: Ezaki
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Itakura
authors.forename: Kiyoko S
authors.initials: KS
authors.lastname: Akagawa
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001095:Arachidonic Acids / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood*; D007700:Kinetics; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009000:Monocytes / Q000166:cytology*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D001095:Arachidonic Acids; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006861:Hydrogen Peroxide