Innervation of guinea-pig stellate ganglia by nitric oxide synthase, cocaine- and amphetamine-regulated transcript protein- and pituitary adenylate cyclase activating polypeptide-immunoreactive fibers.
The present study analyzed using immunohistochemical labeling the distribution and co-localization of nitric oxide synthase (NOS), cocaine- and amphetamine-regulated transcript peptide (CARTp) and pituitary adenylate cyclase activating polypeptide (PACAP) with choline acetyltransferase (ChAT)-immunoreactive fibers in the guinea-pig stellate ganglia. ChAT-immunoreactive fibers make pericellular baskets around virtually all stellate ganglia neurons. Pericellular baskets of NOS, CARTp and PACAP fibers were also present around numerous stellate ganglia neurons. Although all the NOS and PACAP fibers also exhibited ChAT immunoreactivity, only some of the CARTp fibers were ChAT-immunoreactive. No evidence of co-localization of NOS, PACAP and CARTp was obtained.These results indicate that NOS, PACAP and CARTp are present in distinct preganglionic axons innervating the guinea-pig stellate ganglia.
Locknar
S A
SA
Department of Anatomy and Neurobiology, University of Vermont College of Medicine, Burlington, VT 05405-0068, USA.
Calupca
M A
MA
Parsons
R L
RL
D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D018613:Microscopy, Confocal; D009412:Nerve Fibers / Q000378:metabolism* / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D013116:Spinal Cord / Q000378:metabolism; D013233:Stellate Ganglion / Q000378:metabolism* / Q000648:ultrastructure; D014018:Tissue Distribution; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D019001:Nitric Oxide Synthase; D002795:Choline O-Acetyltransferase