title: Pseudomonas aeruginosa galU is required for a complete lipopolysaccharide core and repairs a secondary mutation in a PA103 (serogroup O11) wbpM mutant.
abstract: Insertional inactivation of wbpM in Pseudomonas aeruginosa serogroup O11 strain PA103 resulted in mutants exhibiting three distinct lipopolysaccharide (LPS) phenotypes. One mutant, PA103 wbpM-C, had a truncated LPS core and lacked O antigen. These defects were not complemented by the cloned wbpM gene, suggesting a secondary mutation was present. When the wild-type galU gene was introduced into strain PA103 wbpM-C containing the cloned wbpM gene, both LPS defects were corrected. Construction of galU mutants in P. aeruginosa serogroups O11, O5, O6 and O17 strains led to truncation of the LPS core, indicating the involvement of GalU in P. aeruginosa LPS core synthesis.
authors.affiliation: Department of Microbiology, University of Virginia, Health Sciences Center, P.O. Box 800734, Charlottesville, VA 22908, USA.
authors.forename: Charles R
authors.initials: CR
authors.lastname: Dean
authors.forename: Joanna B
authors.initials: JB
authors.lastname: Goldberg
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test; D006836:Hydro-Lyases / Q000235:genetics*; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009154:Mutation / Q000235:genetics*; D019081:O Antigens / Q000032:analysis; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008070:Lipopolysaccharides; D019081:O Antigens; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase; C121039:galU protein, E coli; D006836:Hydro-Lyases; C441972:wbpM protein, Pseudomonas aeruginosa