Liver necrosis induced by acute intraperitoneal ethanol administration in aged rats.
It is generally agreed that the deleterious pathophysiological effects of ethanol are caused, at least partially by an increase in free radical production. However, little attention has been directed to the effects of ethanol upon elderly organisms. Male Wistar rats at ages 3, 6, 12, 18 and 24 months were treated either with a single i.p. dose of 35% ethanol (v/v) at 3 g ethanol/kg body weight or an isovolumetric amount of 0.9% saline solution. We then assessed the plasma levels of transaminases and hepatic levels of oxidative stress-related parameters, followed by liver histological evaluation. The younger rats (3 months old) were not affected by the treatment with ethanol with respect to any of the studied parameters except for a lowering of total hepatic GSH and an increase in hepatic thiobarbituric acid reactants (TBARS) formation, while animals older than 3 months were increasingly more affected by the treatment. Acute ethanol treatment elicited the similar responses to those in the 3 months-old group, plus a decrease in the hepatic and plasma levels of beta-carotene and the plasma level of alpha-tocopherol, as well as an increase in the activity of plasma transaminases. In the 12,18 and 24 months old groups, there was increasing liver necrosis. These findings suggest that liver damage induced by acute ethanol administration in elderly rats may involve a lack of antioxidants.
Giavarotti
Leandro
L
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil. junqueira-geriat@pesquisa.epm.br
D'Almeida
Vania
V
Giavarotti
Karin A S
KA
Azzalis
Ligia A
LA
Rodrigues
Luciano
L
Cravero
Amerys A M
AA
Videla
Luis A
LA
Koch
Osvaldo R
OR
Junqueira
Virginia B C
VB
D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000032:analysis; D001835:Body Weight; D000431:Ethanol / Q000008:administration & dosage* / Q000097:blood; D001556:Hexachlorocyclohexane / Q000008:administration & dosage / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008104:Liver Cirrhosis, Alcoholic / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009929:Organ Size; D016877:Oxidants / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances; D000637:Transaminases / Q000378:metabolism; D014810:Vitamin E / Q000032:analysis
D000975:Antioxidants; D016877:Oxidants; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D000431:Ethanol; D001556:Hexachlorocyclohexane; D000637:Transaminases