[Fluorescent analysis of cryopreserved totipotent cells of amphibian embryos].
The cryotolerance of totipotent cells from dissociated embryos of amphibian (grass frog Rana temporaria and grey toad Bufo bufo) was studied. Cell integrity and preservation of the cell barrier function were evaluated by fluorescent analysis. It was shown that the best cryopreservation of the cells was achieved by using the cryoprotective agent 10% dimethyl sulfoxide and 10% saccharose. These cells were successfully used for the homotransplantation of nuclei into enucleated eggs. The development of reconstructed eggs to the blastula stage was noted.
Uteshev
V K
VK
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
Mel'nikova
E V
EV
Kaurova
S A
SA
Nikitin
V A
VA
Gakhova
E N
EN
Karnaukhov
V N
VN
D000818:Animals; D002023:Bufo bufo / Q000196:embryology*; D015925:Cryopreservation; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000648:ultrastructure; D005453:Fluorescence; D066298:In Vitro Techniques; D053652:Nuclear Transfer Techniques; D011896:Rana temporaria / Q000196:embryology*; D015053:Zygote
Fluorestsentnyĭ analiz kriokonservirovannykh totipotentnykh kletok zarodysheĭ amfibiĭ.