Cypermethrin-induced plasma membrane perturbation on erythrocytes from rats: reduction of fluidity in the hydrophobic core and in glutathione peroxidase activity.
The effects of treatment with the synthetic insecticide cypermethrin on plasma membrane fluidity, lipid peroxidation and antioxidant status in rat erythrocytes were investigated. Rats were treated by gavage with a low dose (12.5 mg/kg body weight per day) of cypermethrin in corn oil for 60 days. DPH and TMA-DPH fluorescence anisotropy experiments show that cypermethrin treatment, compared with controls, induced a significant decrease in erythrocyte membrane fluidity measured by DPH, while no changes were observed using TMA-DPH. Cypermethrin treatment also induced a significant increase in the lipid peroxidation, measured by the formation of conjugated dienes. The increased oxidative stress resulted in a significant decrease in the activity of glutathione peroxidase. The results are discussed in terms of preferential localization of cypermethrin in the hydrophobic core of the membrane, where it increases lipid packing and consequently decreases membrane fluidity.
Gabbianelli
Rosita
R
Dipartimento di Biologia M.C.A., Università di Camerino (MC), Via M. Scalzino, 3, 62032 Camerino (MC), Italy.
Falcioni
Giancarlo
G
Nasuti
Cinzia
C
Cantalamessa
Franco
F
D000818:Animals; D002374:Catalase / Q000097:blood; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D005454:Fluorescence Polarization; D005979:Glutathione Peroxidase / Q000097:blood*; D007306:Insecticides / Q000633:toxicity*; D008054:Lipid Peroxides / Q000096:biosynthesis; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D008565:Membrane Proteins / Q000378:metabolism; D008706:Methemoglobin / Q000378:metabolism; D011722:Pyrethrins / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood
D007306:Insecticides; D008054:Lipid Peroxides; D008565:Membrane Proteins; D011722:Pyrethrins; C017160:cypermethrin; D008706:Methemoglobin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase