Cadherin-17 is required to maintain pronephric duct integrity during zebrafish development.
We have isolated a zebrafish cadherin that is orthologous to human LI-cadherin (CDH17). Zebrafish cdh17 is expressed exclusively in the pronephric ducts during embryogenesis, and in the mesonephros during larval development and adulthood. Like its mammalian ortholog, cdh17 is also expressed in liver and intestine in adult zebrafish. We show that cdh17-positive mesodermal cells do not contribute to the hematopoietic system. Consistent with a cell adhesion role for Cdh17, depletion of Cdh17 function using antisense morpholino oligonucleotides compromised cell cohesion during pronephric duct formation. Our results indicate that Cdh17 is necessary for maintaining the integrity of the pronephric ducts during zebrafish embryogenesis. This finding contrasts with the role of mammalian CDH17, which does not appear to be involved in nephric development.
Horsfield
Julia
J
Department of Molecular Medicine & Pathology, The University of Auckland, Auckland, New Zealand.
Ramachandran
Anassuya
A
Reuter
Katja
K
LaVallie
Edward
E
Collins-Racie
Lisa
L
Crosier
Kathryn
K
Crosier
Philip
P
D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers; D015820:Cadherins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002352:Carrier Proteins; D004268:DNA-Binding Proteins / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007684:Kidney Tubules / Q000196:embryology*; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D051762:PAX2 Transcription Factor; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics; D025721:WT1 Proteins / Q000235:genetics; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
D015415:Biomarkers; C490584:CDH17 protein, human; C470286:CDH17 protein, zebrafish; D015820:Cadherins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D026901:Membrane Transport Proteins; D051762:PAX2 Transcription Factor; C497318:PAX2 protein, human; D014157:Transcription Factors; D025721:WT1 Proteins; D029961:Zebrafish Proteins; C067802:intestinal peptide-proton cotransporter; C497320:pax2a protein, zebrafish