Clustering of a lipid-raft associated pool of ERM proteins at the immunological synapse upon T cell receptor or CD28 ligation.
Although ezrin is tyrosine phosphorylated following TCR ligation, its biological role in T cell activation is not known. Here, we shhow that ezrin clusters at the immunological synapse upon T cell stimulation. Clustering of ezrin can be triggered by TCR ligation, or, more efficiently, by CD28 ligation. The clusters of ezrin at the immunological synapse include serine/threonine phosphorylated ezrin predominantly located within cell membrane lipid rafts. Based on these data, we propose that ezrin may play a role in the formation/stabilization of lipid raft signalosomes at the immunological synapse and therefore contribute to sustain TCR-dependent signalling.
Tomas
Elizabeth M
EM
The Biotherapeutics, and the Transplantation and Immunobiology Groups, The John P. Robarts Research Institute, Room 2.05, P.O. Box 5015, 100 Perth Drive, London Ont., Canada N6A 5K8.
Chau
Thu A
TA
Madrenas
Joaquín
J
D018106:CD28 Antigens / Q000276:immunology / Q000378:metabolism*; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D021962:Membrane Microdomains / Q000378:metabolism*; D018613:Microscopy, Confocal; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000378:metabolism*; D014157:Transcription Factors / Q000276:immunology / Q000378:metabolism*; D000099149:Ezrin
D018106:CD28 Antigens; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D011948:Receptors, Antigen, T-Cell; D014157:Transcription Factors; D000099149:Ezrin; C086680:ETV5 protein, human