title: Stage-specific chromosomal association of Drosophila dMBD2/3 during genome activation.
abstract: The Drosophila gene dMBD2/3 encodes a protein with significant homologies to the mammalian methyl-DNA binding proteins MBD2 and MBD3. These proteins are essential components of chromatin complexes involved in epigenetic gene regulation. Because the available in vitro data on dMBD2/3 are conflicting we have started an in vivo characterization of dMBD2/3. We detected expression of two isoforms specifically during embryonic development. Staining of whole embryos combined with high-resolution confocal microscopy revealed a highly regulated spatial distribution. During the syncytial blastoderm stage, dMBD2/3 formed speckles that localized to the cytoplasm. Shortly after, during the cellular blastoderm stage, the protein entered the nucleus and formed bright foci that associated with DNA. This rapid transition coincided with the activation of the embryonic genome. A similar observation was made during activation of the spermatocyte genome as dMBD2/3 formed distinct foci associated with the activated Y chromosome. Our results indicate that dMBD2/3 forms specialized nuclear compartments to keep certain genes epigenetically silenced during genome activation.
authors.affiliation: Research Group Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Marhold
authors.forename: Marcin
authors.initials: M
authors.lastname: Zbylut
authors.forename: Dirk-Henner
authors.initials: DH
authors.lastname: Lankenau
authors.forename: Mingfa
authors.initials: M
authors.lastname: Li
authors.forename: Daniel
authors.initials: D
authors.lastname: Gerlich
authors.forename: Esteban
authors.initials: E
authors.lastname: Ballestar
authors.forename: Bernard M
authors.initials: BM
authors.lastname: Mechler
authors.forename: Frank
authors.initials: F
authors.lastname: Lyko
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D029721:Drosophila Proteins / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015151:Immunoblotting; D008297:Male; D018613:Microscopy, Confocal; D011485:Protein Binding; D013090:Spermatocytes / Q000378:metabolism; D014998:Y Chromosome / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C115368:MBD2 protein