title: Yeast one-hybrid system used to identify the binding proteins for rat glutathione S-transferase P enhancer I.
abstract: OBJECTIVE
  To detect the trans-factors specifically binding to the strong enhancer element (GPEI) in the upstream of rat glutathione S-transferase P (GST-P) gene.
  
  
  METHODS
  Yeast one-hybrid system was used to screen rat lung MATCHMAKER cDNA library to identify potential trans-factors that can interact with core sequence of GPEI(cGPEI). Electrophoresis mobility shift assay (EMSA) was used to analyze the binding of transfactors to cGPEI.
  
  
  RESULTS
  cDNA fragments coding for the C-terminal part of the transcription factor c-Jun and rat adenine nucleotide translocator (ANT) were isolated. The binding of c-Jun and ANT to GPEI core sequence were confirmed.
  
  
  CONCLUSIONS
  Rat c-jun transcriptional factor and ANT may interact with cGPEI. They could play an important role in the induced expression of GST-P gene.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China.
authors.forename: Ming-Xiang
authors.initials: MX
authors.lastname: Liao
authors.forename: Dong-Yuan
authors.initials: DY
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Zuo
authors.forename: Fu-De
authors.initials: FD
authors.lastname: Fang
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D004790:Enzyme Induction; D005786:Gene Expression Regulation; D015723:Gene Library; D005982:Glutathione Transferase / Q000235:genetics*; D008168:Lung; D051381:Rats; D017422:Sequence Analysis, DNA; D015003:Yeasts
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D005982:Glutathione Transferase