title: Helicobacter pylori accelerates the biliary epithelial cell proliferation activity in hepatolithiasis.
abstract: OBJECTIVE
  Several authors have reported the presence of H. pylori in the human biliary tract. The aim of this study was to investigate the influence of the presence of H. pylori on the epithelial cell proliferation activity in the biliary tract with hepatolithiasis.
  
  
  METHODS
  A histopathological examination and polymerase chain reaction were used to detect the presence of H. pylori from fourteen patients with hepatolithiasis. The cell proliferation activity in the biliary epithelia was determined using proliferating cell nuclear antigen staining.
  
  
  RESULTS
  A histopathological examination and polymerase chain reaction analysis demonstrated H. pylori to be detected in 5 (37%) and 4 (29%) out of 14 patients, respectively. The proliferating cell nuclear antigen labeling index was significantly higher in the H. pylori-positive patients (28.3%) than in the H. pylori-negative individuals (4.9%).
  
  
  CONCLUSIONS
  H. pylori is present in the biliary tract of patients with hepatolithiasis, while H. pylori promotes the formation of stones in the biliary tract. The development of intrahepatic cholangiocarcinoma might therefore be linked to the presence of H. pylori because of the accelerated activity of cell kinetics in the epithelium of the biliary tract.
authors.affiliation: Department of Surgery II, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan. tkuroki-gi@umin.ac.jp
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Kuroki
authors.forename: Kenzo
authors.initials: K
authors.lastname: Fukuda
authors.forename: Kousyou
authors.initials: K
authors.lastname: Yamanouchi
authors.forename: Tomoo
authors.initials: T
authors.lastname: Kitajima
authors.forename: Sumihiro
authors.initials: S
authors.lastname: Matsuzaki
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Tajima
authors.forename: Junichiro
authors.initials: J
authors.lastname: Furui
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanematsu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001646:Bile / Q000382:microbiology*; D001653:Bile Ducts, Intrahepatic; D002769:Cholelithiasis / Q000382:microbiology*; D004396:Coloring Agents; D005260:Female; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen
chemical_list: D004396:Coloring Agents; D018809:Proliferating Cell Nuclear Antigen