title: Trophic effect of multiple growth factors in amniotic fluid or human milk on cultured human fetal small intestinal cells.
abstract: OBJECTIVE
  To evaluate the role of growth factors in amniotic fluid and in human milk on gastrointestinal adaptation of the fetus and very low-birth-weight infants, the effects of these fluids and multiple growth factors were investigated in a human fetal small intestinal cell line (FHs 74 Int).
  
  
  METHODS
  After FHs 74 Int cells were incubated with amniotic fluid, human milk, or recombinant growth factors, growth-promoting activity was measured by [3H]-thymidine incorporation into cells.
  
  
  RESULTS
  Incubating cells with amniotic fluid or human milk promoted growth dose dependently. Genistein almost completely inhibited growth-promoting activity in amniotic fluid P = 0.002), and growth was partially inhibited by antibodies against epidermal growth factor (EGF) (P = 0.047), insulin-like growth factor-1 (IGF-1, P = 0.047), or fibroblast growth factor (FGF, P = 0.014). This activity in human milk was inhibited almost completely by genistein (P < 0.0001) and partially inhibited by antibodies against EGF (P = 0.036), IGF-1 (P = 0.009), FGF (P = 0.004), hepatocyte growth factor (HGF, P = 0.001), or transforming growth factor-alpha (TGF-alpha, P = 0.001). Although recombinant EGF, IGF-1, FGF, HGF, and TGF-alpha elicited a synergistic trophic response on cultured cells, the response was much less than with amniotic fluid or with human milk.
  
  
  CONCLUSIONS
  In aminiotic fluid and in human milk, EGF, IGF-1, FGF, HGF, and TGF-alpha have a strong trophic effect on immature intestinal cells and may be involved in perinatal gastrointestinal adaptation.
authors.affiliation: Department of Pediatrics, Osaka City University Graduate School of Medicine, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Hirai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ichiba
authors.forename: Mika
authors.initials: M
authors.lastname: Saito
authors.forename: Haruo
authors.initials: H
authors.lastname: Shintaku
authors.forename: Tsunekazu
authors.initials: T
authors.lastname: Yamano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kusuda
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D019833:Genistein / Q000494:pharmacology; D006131:Growth Inhibitors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D006801:Humans; D007231:Infant, Newborn; D019102:Infant, Very Low Birth Weight; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007421:Intestine, Small / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D008895:Milk, Human / Q000737:chemistry*; D016211:Transforming Growth Factor alpha / Q000494:pharmacology; D014316:Tritium
chemical_list: D006131:Growth Inhibitors; D006133:Growth Substances; D016211:Transforming Growth Factor alpha; D014316:Tritium; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D007334:Insulin-Like Growth Factor I; D019833:Genistein