title: Cathepsin D is specifically inhibited by deoxyribonucleic acids.
abstract: A cathepsin D (CD) inhibitor was searched using mouse embryonic fibroblasts deficient for CD. Synthetic DNA fragments specifically inhibited CD activity in a dose-dependent manner, but not the activities of other serine or cysteine proteinases. Cathepsin E activity was also inhibited by DNA fragments when hemoglobin was used as a substrate. CD inhibition by DNA fragments appeared to be electrostatic in nature and dependent on Tm values. Moreover, CD activity was partly inhibited by exogenously ingested DNA fragments, suggesting that DNA fragments with high Tm values are potent inhibitors of CD in vitro and partly in vivo.
authors.affiliation: Department of Cell Biology and Neuroscience, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shibata
authors.forename: Masato
authors.initials: M
authors.lastname: Koike
authors.forename: Satoshi
authors.initials: S
authors.lastname: Waguri
authors.forename: Guiqin
authors.initials: G
authors.lastname: Zhang
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Koga
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Uchiyama
mesh_terms: D000818:Animals; D002402:Cathepsin D / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004247:DNA / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004705:Endocytosis / Q000502:physiology; D005347:Fibroblasts / Q000201:enzymology; D006454:Hemoglobins / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D051379:Mice
chemical_list: D006454:Hemoglobins; D004247:DNA; D002402:Cathepsin D