Effect of dietary calcium on cadmium absorption and retention in suckling rats.
The effect of calcium supplementation on absorption and retention of cadmium in the suckling period was evaluated in Wistar rat pups of both sexes. Animals were maintained in the litters with the mother rats and supplemented with 1%, 3% or 6% calcium (as CaHPO4 x 2H2O) in cow's milk by artificial feeding from day of birth 6 through 14. All rats were exposed to cadmium (as CdCl2 x H2O) either orally or parenterally. Oral cadmium dose of 0.5 mg/kg body weight a day was administered through nine-day period of calcium supplementation and parenteral cadmium dose was injected subcutaneously in a single dose of 0.5 mg Cd/kg body weight prior to calcium supplementation. On experimental day 10 (at the age of pups of 15 days) all animals were killed and the liver, kidneys, brain and carcass (body without organs and skin) were removed for element analyses. Cadmium and essential elements calcium, zinc and iron were analysed in the tissues by atomic absorption spectrometry. Results showed that after oral exposure cadmium concentrations in all calcium-supplemented groups were significantly decreased in the organs and carcass and that the effect was dose-related. No such effect of calcium was found after parenteral cadmium exposure. Calcium supplementation per se significantly increased calcium concentration in the carcass and had no effect on iron in organs and zinc in carcass. It was concluded that calcium supplementation during the suckling period could be an efficient way of reducing oral cadmium absorption and retention without affecting tissue essential trace element concentrations.
Sarić
Marijana Matek
MM
Mineral Metabolism Unit, Institute for Medical Research and Occupational Health, Zagreb, Republic of Croatia.
Blanusa
Maja
M
Piasek
Martina
M
Varnai
Veda Marija
VM
Juresa
Dijana
D
Kostial
Krista
K
D000042:Absorption / Q000187:drug effects; D000284:Administration, Oral; D000818:Animals; D000833:Animals, Suckling; D001923:Brain Chemistry; D002104:Cadmium / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002136:Calcium, Dietary / Q000008:administration & dosage / Q000494:pharmacology*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005260:Female; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D023421:Models, Animal; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution / Q000187:drug effects; D015430:Weight Gain / Q000187:drug effects
D002136:Calcium, Dietary; D002104:Cadmium