title: Sigma B contributes to PrfA-mediated virulence in Listeria monocytogenes.
abstract: Transcription of the Listeria monocytogenes positive regulatory factor A protein (PrfA) is initiated from either of two promoters immediately upstream of prfA (prfAp(1) and prfAp(2)) or from the upstream plcA promoter. We demonstrate that prfAp(2) is a functional sigma(B)-dependent promoter and that a sigB deletion mutation affects the virulence phenotype of L. monocytogenes. Thus, the alternative sigma factor sigma(B) contributes to virulence in L. monocytogenes.
authors.affiliation: Department of Food Science, Cornell University, Ithaca, New York 14853, USA.
authors.forename: Celine A
authors.initials: CA
authors.lastname: Nadon
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Bowen
authors.forename: Martin
authors.initials: M
authors.lastname: Wiedmann
authors.forename: Kathryn J
authors.initials: KJ
authors.lastname: Boor
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology*; D002460:Cell Line; D004195:Disease Models, Animal; D015964:Gene Expression Regulation, Bacterial; D007739:L Cells; D008089:Listeria monocytogenes / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D008088:Listeriosis / Q000382:microbiology; D008264:Macrophages / Q000166:cytology; D051379:Mice; D010454:Peptide Termination Factors; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D010454:Peptide Termination Factors; C079011:SigB protein, Bacteria; D012808:Sigma Factor; D015534:Trans-Activators