Reactivity of alveolar macrophages in lung cancer patients and healthy subjects: surface ICAM-1 after INF-gamma stimulation in vitro.
The linearity of ICAM- I expression on alveolar macrophages (AM) before and after INF-gamma stimulation in healthy and lung cancer subjects were compared. AM were collected by bronchoalveolar lavage and incubated with/without INF-gamma according to standard procedures. The harvested cells were analyzed by flow cytometry using monoclonal antibodies against leucocytes and macrophages. Only viable cells were analyzed. Stimulation with INF-gamma revealed two AM subpopulations of similar size differentiated in the intensity of ICAM-1 expression. They were not distinctly marked in every studied case. Our preliminary results did not confirm the previously reported decreasing reactivity of AMs after INF-gamma stimulation in lung cancer patients.
Hoser
G
G
Department of Clinical Cytology, Medical Centre of Postgraduate Education, Warsaw, Poland. graho@cmkp.edu.pl
Grubek-Jaworska
H
H
Droszcz
P
P
Domagała-Kulawik
J
J
Dabrowska
M
M
Chazan
R
R
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000473:pathology*; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000187:drug effects; D013268:Stimulation, Chemical
D000970:Antineoplastic Agents; D011956:Receptors, Cell Surface; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma