Expression of the interleukin-8 receptors CXCR1 and CXCR2 on cord-blood-derived cultured human mast cells.
BACKGROUND
An increase in mast cell number at sites of inflamed tissues has been observed. However, the expression of CXC chemokine receptors on human mast cells is poorly understood.


METHODS
Cultured human mast cells were raised from human umbilical cord blood cells in the presence of stem cell factor and interleukin-6 (IL-6). The expression of surface chemokine receptors on the mast cells was analyzed by flow cytometry and that of mRNA was examined by the method of reverse transcriptase-polymerase chain reaction (RT-PCR). As functional assays for the receptors, mast cell migration was examined by a microchemotaxis assay and changes in the cytosolic free intracellular calcium concentrations ([Ca2+]i) was measured using fura-2-loaded mast cells, respectively.


RESULTS
Expression of IL-8 receptors CXCR1 and CXCR2 was demonstrated by flow cytometry and of both mRNA by RT-PCR; however, CC chemokine receptors including CCR3 were not expressed on cord-blood-derived cultured human mast cells. IL-8 and its homologues showed chemotactic activity toward them in a dose-dependent manner, and IL-8 induced a dose-dependent rapid and transient increase in [Ca2+]i in the mast cells.


CONCLUSIONS
Our results suggest the surface expression of functional CXCR1 and CXCR2 on cord-blood-derived cultured human mast cells.
Inamura
Hiroaki
H
Gunma Clinical Research Center for Allergy and Regeneration, Showa Hospital, Takasaki, Japan.
Kurosawa
Motohiro
M
Okano
Akira
A
Kayaba
Hiroyuki
H
Majima
Masataka
M
D002118:Calcium / Q000276:immunology; D002614:Chelating Agents / Q000737:chemistry; D002633:Chemotaxis / Q000276:immunology; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D016257:Fura-2 / Q000737:chemistry; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D023062:Receptors, Interleukin-8A / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D023063:Receptors, Interleukin-8B / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002614:Chelating Agents; D012333:RNA, Messenger; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B; D002118:Calcium; D016257:Fura-2