Comparison of coagulation activity tests in vitro for selected biomaterials.
Testing of coagulation induced by external communicating medical devices is an International Standardisation Organization (ISO) requirement for products exposed to human blood. Four categories of tests are indicated by ISO 10993/4: a clotting test (partial thromboplastin time; PTT), thrombin generation, fibrinogen deposition, or measurement of coagulation split products. We evaluated the specificity of these tests on various polymers and found only thrombin generation and fibrinogen binding sensitive and reproducible tests while thrombin generation correlated best with the classical PTT. The thrombin generation assay is therefore the method of choice for testing coagulation induced by biomaterials and medical devices.
van Oeveren
W
W
Department of Biomedical Engineering, University of Groningen, Groningen, The Netherlands.
Haan
J
J
Lagerman
P
P
Schoen
P
P
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001780:Blood Coagulation Tests; D006801:Humans; D066298:In Vitro Techniques; D010314:Partial Thromboplastin Time; D020959:Polyethylene / Q000494:pharmacology; D011138:Polytetrafluoroethylene / Q000494:pharmacology; D011140:Polyurethanes / Q000494:pharmacology; D011143:Polyvinyl Chloride / Q000494:pharmacology; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D001672:Biocompatible Materials; D011140:Polyurethanes; D011138:Polytetrafluoroethylene; D011143:Polyvinyl Chloride; D020959:Polyethylene