VCAM-1-positive microglia target oligodendrocytes at the border of multiple sclerosis lesions.
The distribution and lineage of vascular cell adhesion molecule-1 (VCAM-1)-positive cells was investigated in 43 lesions from the brain tissue of patients with multiple sclerosis (MS). Numerous VCAM-1-positive macrophages/microglia were detected at the edges of MS lesions. Quantitative analysis of 6 active, 7 chronic active, and 4 chronic inactive MS lesions identified most VCAM-1-positive cells at the actively demyelinating borders of active (102/mm3) and chronic active (29/mm3) lesions, but rarely in chronic inactive lesions (4/mm3). Further, approximately 17% of the VCAM-1-positive cells closely apposed or surrounded oligodendrocyte perikarya at the edges of active and chronic active lesions that were sites of ongoing demyelination. Endothelial cells were VCAM-1-negative in both lesion and non-lesion MS brain tissue. This report is the first to document direct microglial interaction with oligodendrocytes in MS.
Peterson
John W
JW
Department of Neurosciences, Lerner Research Institute, The Cleveland Clinic Foundation, Ohio 44195, USA.
Bö
Lars
L
Mörk
Sverre
S
Chang
Ansi
A
Ransohoff
Richard M
RM
Trapp
Bruce D
BD
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies; D002450:Cell Communication; D019070:Cell Lineage; D003711:Demyelinating Diseases / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D039041:Integrin alpha4beta1; D016023:Integrins / Q000378:metabolism; D008297:Male; D017628:Microglia / Q000737:chemistry* / Q000473:pathology*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000473:pathology*; D009836:Oligodendroglia / Q000473:pathology*; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D039041:Integrin alpha4beta1; D016023:Integrins; D016201:Receptors, Lymphocyte Homing; D019010:Vascular Cell Adhesion Molecule-1