Efficiency of lipofection of adherent cells is limited by apoptosis.
Stability of gene expression and transfection efficiency plays the main role in the application of gene transfer method. In somatic cell gene delivery, expression of the gene product is limited by the function of the cell to which it is delivered. In the present study analyzing the lipofected adherent cells, we have shown that lower level of transgene: beta-galactosidase activity at later time period correlated with decrease in cell viability, which was shown to be due to apoptosis. Apoptosis following DNA uptake occurred only when DNA was present during lipofection.
Bednarek
I
I
Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, Sosnowiec, Poland. drIBednarek@farmant.slam.katowice.pl
Czajka
M
M
Wilczok
T
T
D017209:Apoptosis / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008081:Liposomes; D008856:Microscopy, Fluorescence; D008860:Microscopy, Ultraviolet; D010957:Plasmids / Q000235:genetics; D013194:Staining and Labeling; D014162:Transfection / Q000379:methods*; D019076:Transgenes / Q000235:genetics; D001616:beta-Galactosidase / Q000378:metabolism
D008081:Liposomes; D004247:DNA; D001616:beta-Galactosidase