The Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase activity increases in response to starvation and temperature upshift.
We have determined the effect of environmental factors (mild thermal upshift and starvation) on the Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase (cwGAPDH) activity. Temperature upshift (from 28 to 37 degrees C) and/ or starvation (at 28 or 37 degrees C in water) of exponentially growing yeast cells caused an increase in cwGAPDH activity (3 to 5-, and 7 to 8-fold, respectively). This increase in activity did not correlate with an increase in the amount of cwGAPDH protein present, as determined by flow cytometry, immunoelectron microscopy and Western-blotting. These results indicate that thermal upshift and starvation cause an activation of the cwGAPDH in C. albicans cells.
Gil
M L
ML
Departament de Microbiologia i Ecologia, Universitat de València, Burjassot, Spain.
Delgado
M L
ML
Gozalbo
D
D
D002176:Candida albicans / Q000201:enzymology*; D002473:Cell Wall / Q000201:enzymology; D005656:Fungal Proteins / Q000032:analysis; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D013696:Temperature
D005656:Fungal Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases