title: Shear stress mediates tyrosylprotein sulfotransferase isoform shift in human endothelial cells.
abstract: In this study, we examined expression of tyrosylprotein sulfotransferase (TPST) isoforms TPST1 and TPST2 in primary cultures of human umbilical vein endothelial cells. For the first time coexpression of both isoforms is shown in primary human cells. Application of physiological levels of shear stress regulates expression of TPST isoforms in a time- and dose-dependent manner. Sustained application of arterial laminar shear stress causes downregulation of TPST1 mRNA and protein expression, while TPST2 is upregulated. This TPST isoform shift is mediated by different signaling pathways. Shear stress-dependent downregulation of TPST1 involves tyrosine kinase, while upregulation of TPST2 is mediated by a protein kinase C-dependent pathway [corrected].
authors.affiliation: Max Planck Institute for Molecular Physiology, Otto-Hahn-Str. 11, D-44227 Dortmund, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Goettsch
authors.forename: Winfried
authors.initials: W
authors.lastname: Goettsch
authors.forename: Henning
authors.initials: H
authors.lastname: Morawietz
authors.forename: Peter
authors.initials: P
authors.lastname: Bayer
mesh_terms: D002478:Cells, Cultured; D015536:Down-Regulation; D004730:Endothelium, Vascular / Q000201:enzymology* / Q000503:physiopathology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis; D008565:Membrane Proteins; D011493:Protein Kinase C / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D015398:Signal Transduction; D013314:Stress, Mechanical; D015238:Sulfotransferases / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D015238:Sulfotransferases; C114740:TPST2 protein, human; C039079:protein-tyrosine sulfotransferase