Effect of calcitriol on proliferation of TT cells and on expression of calcitonin gene.
The absence of serum steroids was demonstrated to restrict proliferation of cultured TT cells (cell line originating from human thyroid medullary carcinoma) and supplementation with calcitriol was found to partially restore the proliferation. Calcitriol stimulated TT cell proliferation by augmenting expression of proliferation-associated proteins and by restricting apoptosis. Moreover, calcitriol decreased the intensity of transcription but failed to change direction of the altemate splicing of the calcitonin gene.
Zabel
M
M
Department of Histology and Embryology, University Medical School, Wrocław, Poland. mazab@am.wroc.pl
Gebarowska
E
E
Drag-Zalesińska
M
M
Wysocka
T
T
D017209:Apoptosis / Q000187:drug effects; D002116:Calcitonin / Q000096:biosynthesis* / Q000235:genetics; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis; D002117:Calcitriol / Q000494:pharmacology*; D002120:Calcium Channel Agonists / Q000494:pharmacology*; D018276:Carcinoma, Medullary / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D009336:Necrosis; D012333:RNA, Messenger / Q000096:biosynthesis; D013194:Staining and Labeling; D013964:Thyroid Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
D002120:Calcium Channel Agonists; D003470:Culture Media; D012333:RNA, Messenger; D002116:Calcitonin; D002117:Calcitriol; D015740:Calcitonin Gene-Related Peptide