Analysis of grape Vitis vinifera L. DNA in must mixtures and experimental mixed wines using microsatellite markers.
Because wine quality highly relies on the varietal composition of the must, the development of methods allowing the authentication of varieties in musts and wines would be of great value as a guarantee of quality. Microsatellite markers have already been applied to the authentication of grape juices (Faria, M. A.; Magalhães, R.; Ferreira, M. A.; Meredith, C. P.; Ferreira Monteiro, F. J. Agric. Food Chem. 2000, 48, 1096-1100) and to the analysis of experimental wines (Siret, R.; Boursiquot, J. M.; Merle, M. H.; Cabanis, J. C.; This, P. J. Agric Food Chem. 2000, 48, 5035-5040). In the present paper, we accessed the usefulness of this technology for the analysis of must and wine mixtures. The detection limit of DNA mixtures was first estimated on DNA extracted from leaves: 4% of a foreign DNA can be detected. Analysis of must and wine mixtures (Chardonnay B/Clairette B and Syrah N/Grenache N) was performed on experimental fermentations. DNA was extracted along the fermentation process and analyzed using five microsatellite loci. The 70:30 (v/v) mixtures were successfully analyzed until the end of the fermentation. The applications of these results to commercial purposes are discussed.
Siret
René
R
Unité Mixte de Recherches 1097, Diversité et Génomes des Plantes Cultivées (ENSA.M, CIRAD, INRA, IRD), Equipe Génétique Vigne, ENSA.M-INRA, 2 Place P. Viala, 34060 Montpellier, France. siret@ensam.inra.fr
Gigaud
Olivier
O
Rosec
Jean Philippe
JP
This
Patrice
P
D018744:DNA, Plant / Q000032:analysis*; D005285:Fermentation; D005638:Fruit / Q000737:chemistry; D018895:Microsatellite Repeats; D018515:Plant Leaves / Q000737:chemistry; D027843:Vitis / Q000235:genetics*; D014920:Wine / Q000032:analysis* / Q000145:classification
D018744:DNA, Plant