Trypanosoma cruzi heat-shock protein-70 kDa,alone or fused to the parasite KMP11 antigen, induces functional maturation of murine dendritic cells.
We analyse the effect of Trypanosoma cruzi heat-shock protein-70 (HSP70) on the maturation of murine dendritic cells (DC)generated from bone marrow precursor cells. The results obtained show that HSP70, both alone and fused to the KMP11 antigen, as well as a HSP70 fragment, is capable of maturing murine DC. Mature DC have enhanced expression of IL12, TNF-alpha cytokines, costimulation molecules and activation markers, showing a clear increase in the allostimulatory capacity. These findings suggest that T. cruzi HSP70 may be a very useful vehicle for developing DC-based immunoprophylaxis and therapy against infections.
Planelles
L
L
Departamento de BiologíaMolecular, Instituto de Parasitología y Biomedicina 'LópezNeyra', Consejo Superior de Investigaciones Cieníficas(CSIC), Granada, Spain.
Thomas
Mc
M
Pulgar
M
M
Marañón
C
C
Grabbe
S
S
López
M C
MC
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000502:physiology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D011112:Polymyxin B / Q000494:pharmacology; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000276:immunology*
D000953:Antigens, Protozoan; D018840:HSP70 Heat-Shock Proteins; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C090009:kinetoplastid membrane protein 11, Leishmania donovani; D011112:Polymyxin B