Genotoxic effects of ochratoxin A in human-derived hepatoma (HepG2) cells.
Ochratoxin A (OTA) is a widespread mycotoxin that occurs in many commodities from grains to coffee beans all over the world. Evidence is accumulating that OTA may cause cancer in humans. The compound was tested in micronucleus (MN) and single-cell gel electrophoresis (SCGE) assays in human-derived hepatoma (HepG2) cells and caused pronounced dose-dependent effects at exposure concentrations of 5 microg/ml and greater. On the contrary, no induction of His(+) revertants was found in Salmonella microsome assays with strains TA98 and TA100 with HepG2-derived enzyme (S9) mix in liquid incubation assays under identical exposure concentrations. Taken together, our results indicate that OTA is clastogenic in the human-derived cells. These findings support the assumption that this mycotoxin may cause genotoxic effects in hepatic tissue of humans.
Ehrlich
V
V
Institute of Cancer Research, University of Vienna, Borschkegasse 8a, A-1090, Vienna, Austria. veronikaehrlich@hotmail.com
Darroudi
F
F
Uhl
M
M
Steinkellner
H
H
Gann
M
M
Majer
B J
BJ
Eisenbauer
M
M
Knasmüller
S
S
D002273:Carcinogens / Q000633:toxicity*; D006528:Carcinoma, Hepatocellular; D002455:Cell Division / Q000187:drug effects; D020552:Comet Assay; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008113:Liver Neoplasms; D015162:Micronucleus Tests; D009793:Ochratoxins / Q000633:toxicity*; D012475:Salmonella / Q000187:drug effects; D014407:Tumor Cells, Cultured
D002273:Carcinogens; D009793:Ochratoxins; C025589:ochratoxin A