title: Expression of Ets-related transcription factors and matrix metalloproteinase genes in human breast cancer cells.
abstract: The expression levels of ets and MMP genes was examined in two breast cancer cell lines of differing invasive potential. The more invasive MDA-MB-231 cell line had higher levels of Ets-1, Ets-2, PEA3, ERM, Tel, Net, MMP-13 and -14 mRNA than MCF-7 cells. MMP-1, -3 and -16 mRNAs were expressed equally. TPA stimulated MMP-1, -9 and TIMP-1 mRNA expression in both cell lines. MMP-2 and MMP-7 mRNAs were not detected in either cell line. The Ets-1 protein was only detected in MDA-MB-231 cells and its level increased following TPA stimulation. TPA induced MMP-9 activity in MCF-7 cells and increased its activity in MDA-MB-231 cells, however, MMP-2 activity was not detected.
authors.affiliation: Hamilton Regional Cancer Centre, McMaster University, Department of Pathology and Molecular Medicine, Ontario, Canada.
authors.forename: Jane M
authors.initials: JM
authors.lastname: Barrett
authors.forename: Mark A
authors.initials: MA
authors.lastname: Puglia
authors.forename: Gurmit
authors.initials: G
authors.lastname: Singh
authors.forename: Richard G
authors.initials: RG
authors.lastname: Tozer
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: C494041:ETS1 protein, human; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors; D020782:Matrix Metalloproteinases; D020780:Matrix Metalloproteinase 9