RNA helicase activity of the plant virus movement proteins encoded by the first gene of the triple gene block.
Cell-to-cell and long-distance transport of some plant viruses requires coordinated action of three movement proteins encoded by triple gene block (TGB). The largest of TGB proteins, TGBp1, is a member of the superfamily I of DNA/RNA helicases and possesses a set of conserved helicase sequence motifs necessary for virus movement. A recombinant His-tagged form of TGBp1 of two hordeiviruses and potato virus X, a potexvirus, produced in Escherichia coli had unwinding activity on a partially duplexed RNA, but not DNA substrate. The helicase activity of these proteins was dependent on Mg2+ and ATP. The isolated C-terminal half of the PSLV TGBp1 retaining all helicase motifs was also able to unwind RNA duplex.
Kalinina
N O
NO
Department of Virology, Moscow State University, Moscow, Russia.
Rakitina
D V
DV
Solovyev
A G
AG
Schiemann
J
J
Morozov
S Yu
SY
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D004247:DNA / Q000378:metabolism; D005814:Genes, Viral / Q000502:physiology; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000201:enzymology* / Q000235:genetics; D053830:Plant Viral Movement Proteins; D010942:Plant Viruses / Q000201:enzymology / Q000235:genetics; D017863:Potexvirus / Q000201:enzymology / Q000235:genetics; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism*; D012328:RNA Viruses / Q000201:enzymology* / Q000235:genetics; D012330:RNA, Double-Stranded / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D053830:Plant Viral Movement Proteins; D012330:RNA, Double-Stranded; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D004247:DNA; C462509:TGBp1 protein, Poa semilatent virus; D000251:Adenosine Triphosphatases; D020365:RNA Helicases