Complete mapping of a novel HLA A*6801-restricted HIV-1 Tat epitope directly with a rapid modified enzyme-linked immunospot assay.
We identified a novel HLA A*6801-restricted HIV-1 Tat-derived cytotoxic T lymphocyte (CTL) epitope using an adapted enzyme-linked immunospot assay that allows the rapid ex vivo identification of CTL epitopes together with their associated HLA Class I restriction elements. The optimal 11 amino acid residue Tat epitope efficiently stabilized the refolding of monomeric peptide-HLA A6801 complexes in vitro and fluorochrome-labelled, tetrameric peptide-HLA A6801 complexes stained CD8 T cells specific for this epitope directly ex vivo.
Oxenius
Annette
A
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford, UK.
Jakobsen
Bent K
BK
Easterbrook
Philippa J
PJ
Boulter
Jonathan M
JM
Tun
Tin
T
Waters
Anele
A
Agudelo
Juliet
J
Barnardo
Martin
M
Phillips
Rodney E
RE
Price
David A
DA
D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping / Q000379:methods*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015696:Gene Products, tat / Q000737:chemistry / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology*; D006801:Humans; D054322:tat Gene Products, Human Immunodeficiency Virus
D018984:Epitopes, T-Lymphocyte; D015696:Gene Products, tat; D015234:HLA-A Antigens; D054322:tat Gene Products, Human Immunodeficiency Virus