title: Infection and transformation of dendritic cells from bovine afferent lymph by Theileria annulata.
abstract: Following incubation with sporozoites of the protozoan parasite Theileria annulata, dendritic cells (DC), extracted from bovine afferent lymph, became infected and transformed into large, rounded, continuously proliferating cell lines. Phenotypic analysis of the transformed cells by immunostaining and flow cytometry revealed that they expressed MHC class I and II antigens, the myeloid marker MyD (SIRP alpha) and the bovine WC6 (workshop cluster 6) molecule. Transformed DC cell lines differed from those produced from infection of macrophages and B cells in that some lines expressed CD21 and a proportion of cells continued to express the antigen stained by the mAb CC81, a marker which defines a subpopulation of DC in afferent lymph. Both of the main populations of DC that have been identified in bovine afferent lymph appeared to be equally permissive for infection and transformation with T. annulata. These findings raise the possibility that the transformed proliferating cells characteristic of in vivo infections could be derived from DC as well as macrophages. This could have consequences for understanding the pathogenesis of the disease and for developing methods to manipulate immune responses to eliminate the parasite.
authors.affiliation: Institute for Animal Health, Newbury, Berkshire, UK. sue.stephens@bbsrc.ac.uk
authors.forename: S A
authors.initials: SA
authors.lastname: Stephens
authors.forename: C J
authors.initials: CJ
authors.lastname: Howard
mesh_terms: D000818:Animals; D002417:Cattle; D002461:Cell Line, Transformed; D003713:Dendritic Cells / Q000276:immunology / Q000469:parasitology* / Q000502:physiology*; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D008196:Lymph / Q000166:cytology; D008213:Lymphocyte Activation; D016796:Theileria annulata / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D013801:Theileriasis / Q000469:parasitology
chemical_list: D000949:Histocompatibility Antigens Class II