Effect of acid perfusion on passive electrophysiological properties of rabbit esophagus in vivo.
In the present paper we studied early acid-induced changes in the passive electrical properties of the rabbit esophageal epithelium in vivo by measurements of the transluminal potential difference (PD) during acid perfusion and by estimating the transmucosal electrical resistance (Rm) using cable analysis. Perfusion with acid (pH 1) for 45 min produced a rapid (<1 min) negative shift in the lumen-negative PD followed by a slow lumen-negative drift. The acid-induced change in PD was dependent on the accompanying anion, the largest anion (sulfate) producing the largest change. The acid-induced changes in PD were parallelled by reductions in Rm, these reductions also being dependent on the accompanying anion. Interpretation of resistance and net current (estimated by Ohm's law) time curves suggest that the initial acid-induced changes of the PD reflect properties of the naive mucosa whereas the later drift will reflect a diffusion driven increase in transmucosal proton permeability. Further, coapplication of the protective drug sucrose octasulfate attenuated the hydrochloric acid-induced changes of all measured and estimated electrophysiological parameters. The electrophysiological results were to some extent corroborated by light microscopic findings, although no large acid-induced change in mucosal appearance was observed.
Jacobson
Ingemar
I
Integrative Pharmacology, AstraZeneca R&D Mölndal, Sweden.
Poorkhalkali
Nadereh
N
Jönsson-Rylander
Ann-Cathrine
AC
Orlando
Roy C
RC
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D017097:Electric Impedance; D004947:Esophagus / Q000502:physiology*; D017942:Nitric Acid / Q000494:pharmacology*; D011817:Rabbits; D013464:Sulfuric Acids / Q000494:pharmacology*
D013464:Sulfuric Acids; D017942:Nitric Acid; C033158:sulfuric acid