Preparation and characterization of Cryptococcus neoformans synchronous culture.
We have developed a method for preparation of synchronous culture in Cryptococcus neoformans. The method is based on age fractionation of exponentially growing asynchronous culture through differential sedimentation in 10-20% (w/v) lactose gradient. C. neoformans capsule thickness should be reduced to a minimum to ensure most accurate age fractionation, which is necessary to obtain a higher degree of synchrony. The C. neoformans synchronous culture system has revealed important characteristics with respect to cellular morphology, DNA content and cell volume distribution. The method can be used for further cell cycle studies.
Raclavsky
Vladislav
V
Department of Biology, Faculty of Medicine, Palacky University, Hnevotinska 3, CZ-775 15 Olomouc, Czech Republic. rac@tunw.upol.cz
Ohkusu
Misako
M
Hruskova
Pavla
P
Takeo
Kanji
K
D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle; D003455:Cryptococcus neoformans / Q000254:growth & development*; D008856:Microscopy, Fluorescence; D009172:Mycology / Q000379:methods*