title: Larval Taenia crassiceps secretes a protein with characteristics of murine interferon-gamma.
abstract: Previous studies have shown that excretory/secretory products of larval Taenia crassiceps have immunomodulatory activities. We report here that one of these products, termed p66, possesses activities that mimic some characteristics of murine interferon-gamma (IFN-gamma). Purified p66 cross-reacts with anti-murine IFN-gamma on immunoblots and increases concanavalin-A-induced splenic T-cell proliferative responses of normal and chronically infected mice. It has also been shown that p66 induces enhanced IFN-gamma and interleukin-10 (IL-10) production in cells from infected or normal mice. Adherent and non-adherent peritoneal exudates cells were stimulated with p66 and showed that the adherent cells were induced to produce IL-10 and that the non-adherent cells were induced to produce IFN-gamma. p66 was shown as well to upregulate nitric oxide production in macrophages and two T-cell lines, IEL and YAC-1, were shown to be stimulated to produce IFN-gamma and IL-10, respectively, by p66. Although the significance of p66 in immunoregulation is not known, we show here that this molecule mimics characteristics of IFN-gamma.
authors.affiliation: Department of Biology, Wake Forest University, Winston-Salem, NC 27109, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Spolski
authors.forename: P G
authors.initials: PG
authors.lastname: Thomas
authors.forename: E J
authors.initials: EJ
authors.lastname: See
authors.forename: K A
authors.initials: KA
authors.lastname: Mooney
authors.forename: R E
authors.initials: RE
authors.lastname: Kuhn
mesh_terms: D000818:Animals; D002460:Cell Line; D003551:Cysticercosis / Q000276:immunology* / Q000469:parasitology; D000939:Epitopes; D005260:Female; D015801:Helminth Proteins / Q000276:immunology* / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology* / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D007814:Larva / Q000276:immunology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013621:Taenia / Q000254:growth & development* / Q000276:immunology*
chemical_list: D000939:Epitopes; D015801:Helminth Proteins; D007106:Immune Sera; D016753:Interleukin-10; D009569:Nitric Oxide; D007371:Interferon-gamma