Are polyamines involved in olfaction? An EAG and biochemical study in Periplaneta americana antennae.
Polyamines have been implicated in modulation of numerous cell functions. The purpose of this study was to assess the role of polyamines in intracellular regulation of insect antenna. Analysis of study data showed two main findings. First, in vivo treatment with the polyamine synthesis inhibitor alpha-difluoromethyl-ornithine enhanced the sensitivity of male Periplaneta americana antenna to female pheromonal blend. Secondly, polyamine modulated phosphorylation of several antennary proteins including two found exclusively in antenna (30 and 48 kDa). In both of these exclusive antennary proteins, phosphorylation changed after stimulation with the pheromonal blend. These results suggest that polyamines play a regulatory role in detection of female pheromonal blend and that modulation of protein phosphorylation is one of the mechanisms involved in this regulation.
Tirard
Alain
A
CNRS, Laboratoire de Neurobiologie, FRE 2092, 31 chemin Joseph-Aiguier, F-13402 Marseille cedex 20, France. tirard@lnb.cnrs-mrs.fr
Renucci
Marielle
M
Provost
Erick
E
Khlat
Jacqueline
J
Clement
Jean-Luc
JL
D000818:Animals; D001345:Autoradiography; D015317:Biogenic Polyamines / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000502:physiology*; D047390:Casein Kinase II; D000518:Eflornithine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004594:Electrophysiology / Q000379:methods; D005260:Female; D019476:Insect Proteins / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D010526:Periplaneta / Q000502:physiology*; D010675:Pheromones / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012903:Smell / Q000187:drug effects / Q000502:physiology*; D013045:Species Specificity
D015317:Biogenic Polyamines; D019476:Insect Proteins; D010675:Pheromones; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D011493:Protein Kinase C; D000518:Eflornithine