title: Inhibition of cell cycle progression in human leukemia HL-60 cells by esculetin.
abstract: Esculetin, a coumarin compound, was found to inhibit cell growth and cell cycle progression by inducing arrest of the G1 phase in HL-60 cells. To obtain information regarding cell cycle arrest induced by esculetin, we examined its effect on the regulating factors of the G1 phase in the leukemia HL-60 cells treated with esculetin by Western blotting. Our observations were: (1) a distinct increase in the level of hypophosphorylated retinoblastoma protein (pRb), and a reduction in the level of CDK4 after treatment with 100 microM of esculetin for 24 h; (2) a marked up-regulation of p27, and a down-regulation of cyclin D1 after treatment with 100 microM esculetin for 24 h. These results suggest that esculetin can inhibit the growth of human leukemia HL-60 cells by G1 phase cell cycle arrest as a result of inhibited pRb phosphorylation.
authors.affiliation: Institute of Biochemistry, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Chau Jong
authors.initials: CJ
authors.lastname: Wang
authors.forename: Yu Jin
authors.initials: YJ
authors.lastname: Hsieh
authors.forename: Chia Yih
authors.initials: CY
authors.lastname: Chu
authors.forename: Yu Ling
authors.initials: YL
authors.lastname: Lin
authors.forename: Tsui Hwa
authors.initials: TH
authors.lastname: Tseng
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D019938:Cyclin D1 / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D015536:Down-Regulation; D018922:HL-60 Cells; D006801:Humans; D008840:Microfilament Proteins / Q000378:metabolism; D009124:Muscle Proteins; D010766:Phosphorylation; D016160:Retinoblastoma Protein / Q000378:metabolism; D013997:Time Factors; D014468:Umbelliferones / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D008840:Microfilament Proteins; D009124:Muscle Proteins; D016160:Retinoblastoma Protein; C058992:Tagln protein, mouse; D014468:Umbelliferones; D019938:Cyclin D1; D004247:DNA; C007628:esculetin