title: Generation of a cell line with smooth muscle phenotype from hypertrophied urinary bladder.
abstract: We have established a cell line from hypertrophied rabbit urinary bladder smooth muscle (SM) that stably expresses SM myosin (SMM). These cells, termed BSM, are spindle shaped and form swirls, similar to the "hills and valleys" described for cultured aortic SM cells. Western blotting revealed that BSM expresses the amino-terminal SMM heavy chain isoform SM-B, the carboxy-terminal SM1 and SM2 isoforms, and SM alpha-actin. In addition, they express cGMP-dependent protein kinase G, made by contractile SM cells in vitro but not by noncontractile cells synthesizing extracellular matrix. Immunofluorescence studies indicate a homogeneous population of cells expressing alpha-actin and SMM, including the SM-B isoform, and karyotyping demonstrates a stable 4N chromosomal pattern. These cells also express calcium-dependent myosin light chain kinase and phosphatase activity and contract in response to the muscarinic agonist bethanechol. To our knowledge, BSM is the first visceral SM cell line that expresses the SM-B isoform and might serve as a useful model to study the transcriptional regulation of tissue-specific SMM isoforms in differentiation and pathological SM.
authors.affiliation: Division of Urology, University of Pennsylvania, Philadelphia 19104, USA.
authors.forename: Yongmu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Wilfried T
authors.initials: WT
authors.lastname: Weber
authors.forename: Shuqin
authors.initials: S
authors.lastname: Wang
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Wein
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Zderic
authors.forename: Samuel
authors.initials: S
authors.lastname: Chacko
authors.forename: Michael E
authors.initials: ME
authors.lastname: DiSanto
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D018723:Bethanechol / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects; D017869:Cyclic GMP-Dependent Protein Kinases / Q000378:metabolism; D020732:Cytogenetic Analysis; D006984:Hypertrophy; D008297:Male; D009130:Muscle, Smooth / Q000502:physiology*; D009218:Myosins / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D011817:Rabbits; D001743:Urinary Bladder / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology*
chemical_list: D000199:Actins; D018723:Bethanechol; D010770:Phosphotransferases; D017869:Cyclic GMP-Dependent Protein Kinases; D010744:Phosphoric Monoester Hydrolases; D009218:Myosins