DNA microarray analysis of the expression profiles of luteinized granulosa cells as a function of ovarian reserve.
OBJECTIVE
To examine the expression profiles of luteinized granulosa cells isolated from women with normal or diminished ovarian reserve undergoing in vitro fertilization.


METHODS
Expression profiling by complementary DNA microarray analysis.


METHODS
Women undergoing in vitro fertilization-embryo transfer in a university-based fertility clinic.


METHODS
Eighteen women with normal or decreased ovarian reserve.


METHODS
All patients were given gonadotropin stimulation in preparation for IVF with granulosa cells isolated at the time of follicular aspiration.


METHODS
Expression profiles of luteinized granulosa cells isolated from each woman were determined by using DNA microarray analysis.


RESULTS
Changes in patterns of gene expression in granulosa cells were observed between women with normal and diminished ovarian reserve. These genes included several anonymous expressed sequence tags and also expressed sequence tags that correspond to known genes.


CONCLUSIONS
Expression profiling of granulosa cells by DNA microarray may yield signature patterns that reflect the status of ovarian reserve and the competence of granulosa cells.
Chin
Khew-Voon
KV
The Cancer Institute of New Jersey, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901, USA. chinkv@umdnj.edu
Seifer
David B
DB
Feng
Bo
B
Lin
Yong
Y
Shih
Wei-Chung
WC
D000328:Adult; D003338:Corpus Luteum / Q000503:physiopathology*; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006107:Granulosa Cells / Q000502:physiology*; D006801:Humans; D007247:Infertility, Female / Q000235:genetics* / Q000503:physiopathology*; D020411:Oligonucleotide Array Sequence Analysis; D010053:Ovary / Q000503:physiopathology*
D018076:DNA, Complementary