Effects of recombinant staphylokinase on coronary thrombosis in Chinese experimental miniature swine.
OBJECTIVE
To study the effects of recombinant staphylokinase (r-Sak) on coronary thrombosis, cardiac ischemia, and myocardial infarction in Chinese experimental miniature swine.


METHODS
Endarterium was injuried and coronary thrombi were formed gradually through direct electrical stimulation on the coronary artery of Chinese experimental miniature swine. Effects of r-Sak in vitro were studied through coronary pathological section, microkinematography, multi media graphic analysis, epicardial electrogram mapping, myocardium histo chemical stain, serum creatine phosphokinase (CPK), and hemorheology.


RESULTS
r-Sak showed a remarkable effect on coronary thrombus. Compared w ith the control group, the two dosage groups, r-Sak 0.45 mg/kg and 1.35 mg/kg, reduced the transverse section area of coronary thrombus (P<0.01), lessened the degree and range of cardiac ischemia (P<0.01), decreased the myocardial infarction area (P<0.01), the activity of CPK and blood viscosity (P<0.05), restrained platelet adhesion and aggregation, and reduced fibrinogen concentration (P<0.05).


CONCLUSIONS
r-Sak could dissolve coronary thrombus obviously and lessen the pathologic reaction of myocardium.
Liu
Jian-Xun
JX
Department of Basic Medicine Science, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China. jianxun@ht.rol.cn.net
Shang
Xiao-Hong
XH
Fu
Jian-Hua
JH
Li
Xin-Zhi
XZ
Wang
Gang
G
D000818:Animals; D003328:Coronary Thrombosis / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D003402:Creatine Kinase / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D018056:Hemorheology / Q000187:drug effects; D008297:Male; D008666:Metalloendopeptidases / Q000627:therapeutic use*; D009203:Myocardial Infarction / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000627:therapeutic use; D013556:Swine, Miniature
D005343:Fibrinolytic Agents; D011994:Recombinant Proteins; D003402:Creatine Kinase; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus