Cellulose acetate precipitation test in sero-diagnosis of amoebiasis.
Cellulose acetate precipitation (CAP) test for the detection of antibody against Entamoeba histolytica using the axenic antigen, was performed on the 127 serum samples obtained from patients with amoebic liver abscess (14), amoebic hepatitis (21), amoebic dysentry (11), amoebic colitis (31), other parasitic infestations (25) and normal individuals (25). The percent positivity was 100, 95.23, 90.9, 67.74 and 16 and 12 respectively whereas the corresponding figures for the indirect immunoflourescence (IFAT) test were 100, 100, 100, 74.19, 12 and 8 respectively. Although CAP is not as good a test as IFAT, yet it can be recommended for routine testing due to its sensitivity, speed of performance and applicability to a single serum sample.
Kandhari
R
R
Departments of Microbiology and Medicine, Maulana Azad Medical College, New Delhi.
Baveja
U K
UK
Bhatia
R
R
Agarwal
D S
DS
Gupta
P S
PS
D000562:Amebiasis / Q000175:diagnosis* / Q000276:immunology* / Q000469:parasitology; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry*; D004404:Dysentery, Amebic / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology; D004748:Entamoeba histolytica / Q000276:immunology; D006801:Humans; D008101:Liver Abscess, Amebic / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology; D011233:Precipitin Tests / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C005062:acetylcellulose; D002482:Cellulose