Visualization of cell death in vivo with the annexin A5 imaging protocol.
Annexin A5 binds to phosphatidylserine (PS), which is one of the "eat me" signals at the surface of the apoptotic cell. This property has been the driving force for the research of annexin A5 as a probe to measure apoptosis in vitro and in vivo. A non-invasive imaging protocol using annexin A5 has been developed and applied successfully to measure programmed cell death programmed cell death (PCD) in patients. This review highlights the aspects of this development and discusses clinical relevance, limitations and future perspectives of this approach of visualizing cell death.
Reutelingsperger
Chris P M
CP
Department of Biochemistry of the Cardiovascular Research Institute Maastricht, University of Maastricht, P.O. Box 616, The Netherlands. c.reutelingsperger@bioch.unimaas.nl
Dumont
Ewald
E
Thimister
Paul W
PW
van Genderen
Hugo
H
Kenis
Heidi
H
van de Eijnde
Stefan
S
Heidendal
Guido
G
Hofstra
Leo
L
D000818:Animals; D017302:Annexins / Q000378:metabolism*; D017209:Apoptosis; D003952:Diagnostic Imaging; D006338:Heart Neoplasms / Q000473:pathology; D006801:Humans; D023421:Models, Animal; D009203:Myocardial Infarction / Q000473:pathology; D010718:Phosphatidylserines / Q000378:metabolism
D017302:Annexins; D010718:Phosphatidylserines