Establishment and characterization of human metastatic hepatocellular carcinoma cell line.
Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide with a poor prognosis. Recently, we established a HCC cell line from a metastatic HCC tumor. GTG banding analysis was performed and the karyotype showed that this metastatic HCC cell line is a hypertriploid (71-78 chromosomes) with a large marker chromosome containing a long homogeneously staining region (hsr). Comparative genomic hybridization was applied to characterize the chromosomal alterations in this metastatic HCC cell line. The results showed that the hsr was composed of amplified DNA sequences from 11q13. Further characterization of the hsr may lead to the isolation of the putative amplified oncogene at 11q13.
Wen
Jian Ming
JM
Department of Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou, China.
Huang
Jie Fu
JF
Hu
Liang
L
Wang
Wei Sheng
WS
Zhang
Meng
M
Sham
Jonathan S T
JS
Xu
Jian Min
JM
Zeng
Wei Fen
WF
Xie
Dan
D
Liang
Li Jian
LJ
Guan
Xin Yuan
XY
D000782:Aneuploidy; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology / Q000556:secondary*; D002871:Chromosome Banding; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics / Q000648:ultrastructure; D005784:Gene Amplification; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D011169:Portal Vein; D014407:Tumor Cells, Cultured; D019043:Vascular Neoplasms / Q000235:genetics / Q000473:pathology / Q000556:secondary*