title: Genome sequence survey identifies unique sequences and key virulence genes with unusual rates of amino Acid substitution in bovine Staphylococcus aureus.
abstract: Staphylococcus aureus is a major cause of mastitis in bovine and other ruminant species. We here present the results of a comparative genomic analysis between a bovine mastitis-associated clone, RF122, and the recently sequenced human-associated clones, Mu50 and N315, of Staphylococcus aureus. A shotgun sequence survey of approximately 10% of the RF122 genome identified numerous unique sequences and those with elevated rates of nonsynonymous substitution. Taken together, these analyses show that there are notable differences in the genomes of bovine mastitis-associated and human clones of S. aureus and provide a framework for the identification of specific factors associated with host specificity in this major human and animal pathogen.
authors.affiliation: Department of Veterinary Pathobiology and Biomedical Genomics Center, University of Minnesota, St. Paul, Minnesota 55108, USA.
authors.forename: Lisa L
authors.initials: LL
authors.lastname: Herron
authors.forename: Rajit
authors.initials: R
authors.lastname: Chakravarty
authors.forename: Christopher
authors.initials: C
authors.lastname: Dwan
authors.forename: J Ross
authors.initials: JR
authors.lastname: Fitzgerald
authors.forename: James M
authors.initials: JM
authors.lastname: Musser
authors.forename: Ernest
authors.initials: E
authors.lastname: Retzel
authors.forename: Vivek
authors.initials: V
authors.lastname: Kapur
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D002417:Cattle; D005260:Female; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006801:Humans; D008414:Mastitis, Bovine / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence