Effect of arbuscular mycorrhizal fungi and soil phosphorus level on expression of protein and activity of peroxidase on passion fruit roots.
The effects of mycorrhizal inoculation and increasing soil P levels on the expression of total proteins and peroxidase activity on passion fruit roots were evaluated. The experimental design was entirely at random, with four treatments of inoculation (a--control; b--Gigaspora albida; c--Scutellospora heterogama; d--mixture of G. albida, G. margarita, S. heterogama, and Glomus clarum) x three levels of soil P (4, 11, and 30 mg/dm3 of soil), each with three replicates. Plants were harvested 70 days after inoculation, when root colonization, shoot P level, protein content, and enzymatic activity of peroxidase (PAGE--7%) on root extract were evaluated. Regarding protein, there was no significant difference among the treatments, except between those roots receiving mixed inoculum and 11 mg P/dm3 of soil. Effect of P on protein concentration, when compared with the inoculation effect was observed. For peroxidase, there was an eletrophoretic band common to all treatments (rf: 0.43) and another that was absent only in noncolonized plants, grown in soil with lower P (rf: 0.46). Mycorrhizal specific bands were not present but a small decrease of intensity of bands in noncolonized plants was observed. Conversely, the control roots presented a single band (rf: 0.33) not observed in the other extracts, that may demonstrate an inhibitory effect of AMF on some host activities. The data showed the influence of P level in soil on the protein expression of roots, suggesting the influence of this nutrient on root genetic expression as well as on the mechanisms of symbiotic control/recognition.
Santos
B A
BA
Departamento de Micologia, Universidade Federal de Pernambuco, Cidade Universitária, Recife, PE, Brazil.
Maia
L C
LC
Cavalcante
U M
UM
Correia
M T
MT
Coelho
L C
LC
D005658:Fungi; D029598:Passiflora / Q000378:metabolism* / Q000382:microbiology; D009195:Peroxidase / Q000378:metabolism*; D010758:Phosphorus / Q000032:analysis / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology
D010940:Plant Proteins; D012987:Soil; D010758:Phosphorus; D009195:Peroxidase