title: Hypertonic sabouraud broth as a simple and powerful test for Candida dubliniensis screening.
abstract: We developed a new screening test for C. dubliniensis based on its inability to grow on Sabouraud dextrose broth with 6.5% NaCl. A total of 266 clinical yeast isolates and 3 reference strains were tested, including 250 C. albicans and 19 C. dubliniensis strains. All C. albicans isolates tested exhibited significant growth on hypertonic Sabouraud broth up to 96 h, while, all C. dubliniensis isolates did not exhibit any visually detectable growth during the same period.
authors.affiliation: Department of CIinical and Toxicological Analysis, Universidade Federal de Santa Maria, RS, Santa Maria, Brazil.
authors.forename: Sydney Hartz
authors.initials: SH
authors.lastname: Alves
authors.forename: Eveline Pipolo
authors.initials: EP
authors.lastname: Milan
authors.forename: Priscilla
authors.initials: P
authors.lastname: de Laet Sant'Ana
authors.forename: Loiva O
authors.initials: LO
authors.lastname: Oliveira
authors.forename: Janio M
authors.initials: JM
authors.lastname: Santurio
authors.forename: Arnaldo Lopes
authors.initials: AL
authors.lastname: Colombo
mesh_terms: D002175:Candida / Q000145:classification* / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D002176:Candida albicans / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D002177:Candidiasis / Q000175:diagnosis* / Q000382:microbiology*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology*; D005947:Glucose; D006801:Humans; D006982:Hypertonic Solutions; D016533:Mycological Typing Techniques / Q000379:methods*; D009994:Osmolar Concentration; D012680:Sensitivity and Specificity; D012965:Sodium Chloride; D013045:Species Specificity; D013997:Time Factors
chemical_list: D003470:Culture Media; D006982:Hypertonic Solutions; D012965:Sodium Chloride; D005947:Glucose