De Novo Interpretation of MS/MS Spectra and Protein Identification via Database Searching.
Peptide sequencing via tandem mass spectrometry(MS/MS)is one of the most powerful tools in proteomics to identify proteins. A new algorithm was developed for de novo interpretation of MS/MS spectra using graph theory and dynamic alignment between real spectra and theoretical spectra. The trustworthy peptides from de novo interpretation were used in protein identification via database searching. A high throughput statistical analysis of SwissProt and TrEMBL protein databases showed that it's enough to identify a protein in database with three sequence tags of four amino acid residues, two sequence tags of five amino acid residues or one sequence tag of eight amino acid residues.
Sheng
Quan-Hu
QH
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. dingdafu@server.shcnc.ac.cn
Xie
Tao
T
Ding
Da-Fu
DF