title: Priming of dolphin neutrophil respiratory burst by recombinant tumor necrosis factor alpha.
abstract: We studied the effects of recombinant dolphin tumor necrosis factor alpha (rdoTNFalpha) on the respiratory burst activity of dolphin neutrophils. rdoTNFalpha enhanced the luminol-dependent chemiluminescence response of dolphin neutrophils induced by concanavalin-A, opsonized zymosan, and heated plasma, but not that induced by phorbol myristate acetate. The TNF-associated priming activity was concentration- and preincubation time-dependent, and heat-instable. These data suggest that, as in human neutrophils, TNFalpha enhances the respiratory burst in dolphin neutrophils that follows short-term incubation with various receptor-mediated agonists.
authors.affiliation: Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan. takuya@brs.nihon-u.ac.jp
authors.forename: Takuya
authors.initials: T
authors.lastname: Itou
authors.forename: Youko
authors.initials: Y
authors.lastname: Shoji
authors.forename: Rikiya
authors.initials: R
authors.lastname: Shiraishi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sugisawa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Endo
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Inoue
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000818:Animals; D004289:Dolphins; D009504:Neutrophils / Q000187:drug effects*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha