Marek's disease in turkeys. II. Characterization of the viral glycoprotein B gene and antigen of a turkey strain of Marek's disease virus.
Marek's disease virus (MDV) causes immunosuppression and tumors in chickens. As sporadic cases of Marek's disease (MD) were recorded in turkeys, the antigenic and genomic characteristics of the MDV glycoprotein B (gB) gene and antigen of turkeys were compared to the chicken MDV gB. The whole chicken and turkey gB genes were sequenced and found identical. By immunoblotting of infected-cell culture lysates using chicken convalescent and gB monoclonal antibodies, the antigenic epitopes of the chicken and turkey viruses were found to differ. The turkey MDV had a unique epitope, compared to the chicken MDV and compared with our previous findings. While the chicken MDV had two epitope types, heat-labile but dithiothreitol (DTT)-stable and heat-stable but DTT-labile, the turkey MDV gB epitope is both heat and DTT-labile.
Davidson
Irit
I
Division of Avian and Fish Diseases, Kimron Veterinary Institute, Bet Dagan, Israel.
Malkinson
M
M
Weisman
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D002645:Chickens; D004279:DNA, Viral / Q000737:chemistry; D000939:Epitopes / Q000032:analysis; D005784:Gene Amplification; D008381:Herpesvirus 2, Gallid / Q000235:genetics* / Q000276:immunology*; D008380:Marek Disease / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D014422:Turkeys; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins; C072811:glycoprotein B, Marek's disease virus