Arginase expression in mouse embryonic development.
We are using the model of the developing mouse embryo to elucidate the pattern of arginase expression in mammalian cells in normal animals and in arginase I (AI) deficiency during development by digoxigenin-labeled RNA in situ hybridization. Our goal is to understand the regulation of these isozymes, with the expectation that this knowledge will help patients suffering from AI deficiency. We found that AI mRNA was widely and strongly expressed in the normal developing mouse embryo; in contrast, a relatively strong AII mRNA signal was found only in the intestine. In the AI knockout mouse embryo, no AII overexpression was found. These results indicated that arginases are needed in mouse embryonic development and AI is the principal form required. The strong AI expression in the peripheral nervous system suggests that the pathogenesis of the neurological retardation in AI deficiency may be conditioned by AI deficiency in the nervous system during embryonic development.
Yu
Hong
H
Mental Retardation Research Center, UCLA, 760 Westwood Plaza, Los Angeles, CA 90024-1759, USA.
Iyer
Ramaswamy K
RK
Yoo
Paul K
PK
Kern
Rita M
RM
Grody
Wayne W
WW
Cederbaum
Stephen D
SD
D000818:Animals; D001119:Arginase / Q000235:genetics*; D002490:Central Nervous System / Q000196:embryology / Q000201:enzymology; D004064:Digestive System / Q000196:embryology / Q000201:enzymology; D004195:Disease Models, Animal; D005314:Embryonic and Fetal Development; D015870:Gene Expression; D020162:Hyperargininemia / Q000201:enzymology / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D007962:Leukocytes / Q000201:enzymology; D051379:Mice; D018345:Mice, Knockout; D017933:Peripheral Nervous System / Q000196:embryology / Q000201:enzymology; D012333:RNA, Messenger; D014018:Tissue Distribution
D007527:Isoenzymes; D012333:RNA, Messenger; D001119:Arginase