A novel transactivating factor that regulates interferon-gamma-dependent gene expression.
We have previously identified a novel interferon (IFN)-stimulated cis-acting enhancer element, gamma-IFN-activated transcriptional element (GATE). GATE differs from the known IFN-stimulated elements in its primary sequence. Preliminary analysis has indicated that the GATE-dependent transcriptional response requires the binding of novel transacting factors. A cDNA expression library derived from an IFN-gamma-stimulated murine macrophage cell line was screened with a (32)P-labeled GATE probe to identify the potential GATE-binding factors. A cDNA coding for a novel transcription-activating factor was identified. Based on its discovery, we named it as GATE-binding factor-1 (GBF-1). GBF-1 homologs are present in mouse, human, monkey, and Drosophila. It activates transcription from reporter genes carrying GATE. It possesses a strong transactivating activity but has a weak DNA binding property. GBF-1 is expressed in most tissues with relatively higher steady-state levels in heart, liver, kidney, and brain. Its expression is induced by IFN-gamma treatment. GBF-1 is present in both cytosolic and nuclear compartments. These studies thus identify a novel transactivating factor in IFN signaling pathways.
Hu
Junbo
J
Marlene and Stewart Greenebaum Cancer Center, Department of Microbiology and Immunology, Molecular and Cellular Biology Program, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
Meng
Qingjun
Q
Roy
Sanjit K
SK
Raha
Abhijit
A
Hu
Jiadi
J
Zhang
Jun
J
Hashimoto
Katsuyuki
K
Kalvakolanu
Dhananjaya V
DV
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology*; D019746:Intramolecular Oxidoreductases; D051379:Mice; D008969:Molecular Sequence Data; D000071876:Prostaglandin-E Synthases; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D017931:DNA Primers; D018076:DNA, Complementary; D015534:Trans-Activators; D007371:Interferon-gamma; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases