title: Regulation of alternative oxidase activity during phosphate deficiency in bean roots (Phaseolus vulgaris).
abstract: Cyanide-resistant respiration was studied in mitochondria isolated from the roots of bean plants (Phaseolus vulgaris L. cv. Złota Saxa) grown hydroponically up to 16 days on a phosphate-sufficient (+P, control) or phosphate-deficient (-P) medium. Western blotting indicated that the alternative oxidase (AOX) was present only in its reduced (active) form, both in phosphate-sufficient and phosphate-deficient roots, but in the latter, the amount of AOX protein was greater. Addition of pyruvate to the isolation, washing and reaction media made mitochondria from +P roots cyanide-insensitive, similar to mitochondria from -P roots. The doubled activity of NAD-malic enzyme (NAD-ME) in -P compared with +P root mitochondria may suggest increased pyruvate production in -P mitochondria. Lower cytochrome c oxidase (COX) activity and no uncoupler effect on respiration indicated limited cytochrome chain activity in -P mitochondria. In -P mitochondria, the oxygen uptake decreased and the level of Q reduction increased from 60 to 80%. With no pyruvate present (AOX not fully activated), inhibition of the cytochrome pathway resulted in an increased level of the ratio of reduced ubiquinone (Qr) to total ubiquinone (Qt) (Qr/Qt) in +P mitochondria, but did not change Qr/Qt in -P mitochondria. When pyruvate was present, the kinetics for AOX were similar in mitochondria from -P and +P roots. It is suggested that AOX participation in -P respiration may provide an acclimation to phosphate deficiency. Stabilization of the ubiquinone reduction level by AOX might prevent the harmful effect of an increased formation of reactive oxygen species.
authors.affiliation: Institute of Experimental Plant Biology, University of Warsaw, Miecznikowa 1, PL-02-096 Warsaw, Poland Department of Molecular Cell Physiology, Free University, De Boelelaan 1087, NL-1081 HV Amsterdam, The Netherlands.
authors.forename: Izabela M.
authors.initials: IM
authors.lastname: Juszczuk
authors.forename: Anneke M.
authors.initials: AM
authors.lastname: Wagner
authors.forename: Anna M.
authors.initials: AM
authors.lastname: Rychter