title: Polyphenols in red wines prevent NADH oxidation induced by peroxynitrite.
abstract: It is known that red wines have antioxidant properties owing to the presence of polyphenols and hydroxycinnamates. It has been reported that these compounds are efficient scavengers of peroxynitrite (ONOO(-)). We assayed the ONOO(-)-scavenging activity of plant polyphenols, hydroxycinnamates, and red wines using a fluormetric assay that involves the participation of ONOO(-) (200 microM) as oxidant and NADH (50-100 microM) as a target molecule. NADH oxidation was prevented by pure polyphenols and hydroxycinnamates. With the wines, the Cabernet Sauvignon showed more potent ONOO(-)-scavenging ability than the Malbecs and the blended wines. These results correlate well with the total phenol content determined by Folin-Ciocalteau assay.
authors.affiliation: Laboratory of Free Radical Biology, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina. lbvaldez@ffyb.uba.ar
authors.forename: Laura B
authors.initials: LB
authors.lastname: Valdez
authors.forename: Lucas
authors.initials: L
authors.lastname: Actis-Goretta
authors.forename: Alberto
authors.initials: A
authors.lastname: Boveris
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D003373:Coumaric Acids / Q000494:pharmacology; D005419:Flavonoids; D016166:Free Radical Scavengers / Q000494:pharmacology*; D009243:NAD / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D016877:Oxidants / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D030421:Peroxynitrous Acid / Q000494:pharmacology*; D010636:Phenols / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D059808:Polyphenols; D014920:Wine
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; D005419:Flavonoids; D016166:Free Radical Scavengers; D016877:Oxidants; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D009243:NAD; D030421:Peroxynitrous Acid