Inhibition of superoxide dismutase by 2-methoxyoestradiol analogues and oestrogen derivatives: structure-activity relationships.
Superoxide dismutases catalyse the dismutation of highly reactive superoxide ions to produce hydrogen peroxide and several lines of evidence suggest that these enzymes play important roles in the development and response to treatment of human cancers. For example, Mn-containing superoxide dismutase is frequently overexpressed in various cancer types and can contribute to resistance to apoptosis. 2-Methoxyoestradiol is a naturally occurring metabolic product of 17beta-oestradiol that inhibits tubulin polymerization and possesses growth inhibitory and cytotoxic activity in vitro and in vivo. More recently 2-methoxyoestradiol has also been shown to inhibit superoxide dismutase (SOD) in a tetrazolium salt based enzyme assay, suggesting that oestrogen derivatives might be useful starting points for the development of effective, non-toxic enzyme inhibitors. Here we have tested the SOD inhibiting activity of a range of oestrogen derivatives to determine structural features important for enzyme inhibition.
Wood
L
L
Cancer Sciences Research Division, School of Medicine, University of Southampton, Southampton General Hospital, UK.
Leese
M R
MR
Leblond
B
B
Woo
L W
LW
Ganeshapillai
D
D
Purohit
A
A
Reed
M J
MJ
Potter
B V
BV
Packham
G
G
D000077584:2-Methoxyestradiol; D000465:Algorithms; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D045166:Estradiol Congeners / Q000494:pharmacology*; D008968:Molecular Conformation; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013455:Sulfur / Q000737:chemistry; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors*; D013481:Superoxides / Q000378:metabolism
D004791:Enzyme Inhibitors; D045166:Estradiol Congeners; D013481:Superoxides; D004958:Estradiol; D000077584:2-Methoxyestradiol; D013455:Sulfur; D013482:Superoxide Dismutase