In situ analysis of enzymes involved in sucrose to hexose-phosphate conversion during stolon-to-tuber transition of potato.
An in situ study of enzymes involved in sucrose to hexose-phosphate conversion during in vitro stolon-to-tuber transition of potato (Solanum tuberosum L. cv. Bintje) was employed to follow developmental changes in spatial patterns. In situ activity of the respective enzymes was visualized by specific activity-staining techniques and they revealed distinct spatially and developmentally regulated patterns. Two of the enzymes studied were also subject to in situ investigations at the transcriptional level. During the stages of stolon formation high hexokinase (EC 2.7.1.1) and acid (cell wall-bound) invertase (EC 3.2.1.26) activities were restricted to the mitotically active (sub)apical region, suggesting a possible importance of these enzymes for cell division. At the onset of tuberization sucrose synthase (EC 2.4.1.13) and fructokinase (EC 2.7.1.4) were strongly induced (visualized at transcriptional and translational level) and the acid invertase activities disappeared from the swelling subapical region as expected. The high degree of similarity in the spatial pattern and the temporal induction of sucrose synthase and fructokinase suggests a tightly co-ordinated coarse (up)regulation, which may be subject to a sugar-modulated mechanism(s) by which genes involved in the metabolic sucrose-starch converting potential are co-ordinately regulated during tuber growth. The overall activity of uridine-5-diphosphoglucose pyrophosphorylase (EC 2.7.7.9) was present in all tissues during stolon and tuber development, implying that its coarse control is not subject to (in)direct developmental regulation.
Appeldoorn
Niek J.G
NJ
The Graduate School Experimental Plant Sciences, Laboratory of Plant Breeding, Wageningen University, Binnenhaven 5, 6709 PD Wageningen, The Netherlands The Graduate School Experimental Plant Sciences, Laboratory of Plant Physiology, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127 276 Moscow, Russia.
Sergeeva
Lidiya
L
Vreugdenhil
Dick
D
Van Der Plas
Linus H.W
LH
Visser
Richard G.F
RG