title: Release of NO by a nitrosyl complex upon activation by the mitochondrial reducing power.
abstract: The reaction of trans-[Ru(NH(3))(4)P(OEt)(3)NO](3+) and mitochondria was investigated through differential pulse polarography and fluorimetry. The nitrosyl complex undergoes one-electron reduction centered on the NO ligand site. The reaction between the mitochondrial reductor and trans-[Ru(NH(3))(4)P(OEt)(3)NO](3+) exhibits a second order specific rate constant calculated as k=2 x 10(1) M(-1) s(-1). The reduced species, trans-[Ru(NH(3))(4)P(OEt)(3)NO](2+), quickly releases NO, yielding trans-[Ru(NH(3))(4)P(OEt)(3)H(2)O](2+). The low toxicities of both trans-[Ru(NH(3))(4)P(OEt)(3)(NO)](2+) and trans-[Ru(NH(3))(4)P(OEt)(3)H(2)O](2+) and its ability to release NO after reductive activation in a biological medium make the nitrosyl compound a useful model of a hypotensive drug.
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador São Carlense 400, CP 780, 13560-970, Brazil.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Toledo
authors.forename: Luiz Gonzaga de França
authors.initials: LG
authors.lastname: Lopes
authors.forename: Armindo Antonio
authors.initials: AA
authors.lastname: Alves
authors.forename: Lucia
authors.initials: L
authors.lastname: Pereira da Silva
authors.forename: Douglas Wagner
authors.initials: DW
authors.lastname: Franco
mesh_terms: D000818:Animals; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D009942:Organometallic Compounds / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D019163:Reducing Agents / Q000378:metabolism*; D017975:Ruthenium Compounds / Q000378:metabolism*
chemical_list: D009942:Organometallic Compounds; D019163:Reducing Agents; D017975:Ruthenium Compounds; D009243:NAD; D009569:Nitric Oxide