title: Structure at 1.9 A resolution of a quinohemoprotein alcohol dehydrogenase from Pseudomonas putida HK5.
abstract: The type II quinohemoprotein alcohol dehydrogenase of Pseudomonas putida is a periplasmic enzyme that oxidizes substrate alcohols to the aldehyde and transfers electrons first to pyrroloquinoline quinone (PQQ) and then to an internal heme group. The 1.9 A resolution crystal structure reveals that the enzyme contains a large N-terminal eight-stranded beta propeller domain (approximately 60 kDa) similar to methanol dehydrogenase and a small C-terminal c-type cytochrome domain (approximately 10 kDa) similar to the cytochrome subunit of p-cresol methylhydoxylase. The PQQ is bound near the axis of the propeller domain about 14 A from the heme. A molecule of acetone, the product of the oxidation of isopropanol present during crystallization, appears to be bound in the active site cavity.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Chen
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Yamashita
authors.forename: Taka-aki
authors.initials: TA
authors.lastname: Fujii
authors.forename: Hirohide
authors.initials: H
authors.lastname: Toyama
authors.forename: Osao
authors.initials: O
authors.lastname: Adachi
authors.forename: Henry D
authors.initials: HD
authors.lastname: Bellamy
authors.forename: F Scott
authors.initials: FS
authors.lastname: Mathews
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D001400:Azurin / Q000378:metabolism; D020134:Catalytic Domain; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000378:metabolism; D008024:Ligands; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D017434:Protein Structure, Tertiary; D016958:Pseudomonas putida / Q000737:chemistry* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D008024:Ligands; D001400:Azurin; D000429:Alcohol Oxidoreductases; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; C019641:alcohol dehydrogenase (acceptor)