Human neutrophil antigen-2a expression on neutrophils from healthy adults in western Japan.
BACKGROUND
Human neutrophil antigen-2a (HNA-2a)-specific MoAb, which is necessary to prepare panel neutrophils and to determine the phenotype of patients' neutrophils, has been produced in many laboratories in Japan. The frequency of the HNA-2a-positive population in western Japan is unknown.


METHODS
A cell line secreting an HNA-2a-specific MoAb, TAG4, was established. TAG4 was characterized by flow cytometry, and the reactivities of TAG4 and another HNA-2a-specific MoAb, 7D8, were compared in an inhibition assay and two-color flow cytometry. TAG4 was tested against granulocytes from 408 healthy adult volunteers in western Japan.


RESULTS
TAG4 reacted with neutrophils, but not with the other blood cells. The reactivity of TAG4 with granulocytes treated with glycosyl-phosphatidylinositol-specific phospholipase C was markedly reduced. The reactivity of TAG4 and 7D8 was similar, but 7D8 did not prevent TAG4 from binding. A total of 406 of 408 donors were TAG4 positive. The mean percentage of HNA-2a expression of females was 70.2 percent, and that of males was 66.8 percent.


CONCLUSIONS
TAG4 and 7D8 seem to bind to different epitopes of HNA-2a. The frequency of the HNA-2a-positive population is 99.5 percent of healthy adults in western Japan. The mean percentage of HNA-2a expression of females was greater than in males.
Taniguchi
Kikuyo
K
Hiroshima College of Medical Technology, Hatsukaichi, Japan. tkiku@mth.biglobe.ne.jp
Kobayashi
Masao
M
Harada
Hironori
H
Hiraoka
Asako
A
Tanihiro
Misae
M
Takata
Noboru
N
Kimura
Akiro
A
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D002460:Cell Line; D000939:Epitopes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D005787:Gene Frequency; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D007519:Isoantigens / Q000097:blood* / Q000235:genetics / Q000276:immunology; D007564:Japan; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007519:Isoantigens; C072753:neutrophil-specific antigen NA2