Use of a linear gradient flow program for liquid chromatography-mass spectrometry protein-binding studies.
A rapid screening method to measure drug-protein binding using an immobilized human serum albumin (HSA) column was developed. This method utilizes a linear gradient flow-rate to accelerate the elution of strong binders to the HSA column. Post-column addition of a pressure relief valve enables mass spectrometric detection at relatively high mobile phase flow-rates (i.e., 2 ml/min).
Liu
Hanlan
H
Discovery Analytical Chemistry, Wyeth-Ayerst Research, Pearl River, NY 10965, USA. liuh2@war.wyeth.com
Sabus
Chantel
C
Carter
Guy T
GT
Tischler
Mark
M
D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D011485:Protein Binding