title: The effects of vitamin E on the proliferation and viability of irradiated fibroblast-like cells.
abstract: Few studies have indicated the use of antioxidants in the protection against cancers. The goal of this study was to develop a tissue culture model using MRC-5 cells, which would allow for rapid testing of treatments for skin damage, mediated by exposure to ultraviolet radiation (UVR). Overall the specific aims were to evaluate the morphological and pathophysiological responses of MRC-5 fibroblast cells exposed to UVR and antioxidant supplementation. In the first phase, MRC-5 cells were exposed to UVR for 30, 45 or 60 minute time intervals, and cellular damage was assessed biochemically and morphologically. In the second phase, cells were treated for 45 minutes in the presence or absences of physiological levels of vitamin E. The cells were evaluated after 24, 48, and 72 hours for biochemical and morphological changes. Structural changes were noted throughout the experimental phase among the UVR exposed groups compared with native control. However, treatment with vitamin E pre and post exposure provided a protective role by demonstrating lower levels of MDA (p < 0.05) as well as normal structural appearance.
authors.affiliation: Clinical Health Sciences Graduate Program, School of Health Related Professions, Department of Pathology and Orthopedic Surgery and Rehab, University of Mississippi Medical Center, Jackson, Mississippi, USA.
authors.forename: Pamala
authors.initials: P
authors.lastname: Jones
authors.forename: Hamed
authors.initials: H
authors.lastname: Benghuzzi
authors.forename: Michelle
authors.initials: M
authors.lastname: Tucci
mesh_terms: D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D011506:Proteins / Q000378:metabolism; D014466:Ultraviolet Rays; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D011506:Proteins; D014810:Vitamin E; D005979:Glutathione Peroxidase