title: Analysis of nuclear protein import and export in vitro using fluorescent cargoes.
authors.affiliation: Abteilung Virologie, Universität Heidelberg, Heidelberg, Germany.
authors.forename: Ralph H
authors.initials: RH
authors.lastname: Kehlenbach
authors.forename: Larry
authors.initials: L
authors.lastname: Gerace
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology*; D001681:Biological Assay / Q000379:methods*; D002232:Carbocyanines / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008856:Microscopy, Fluorescence / Q000379:methods; D050778:NFATC Transcription Factors; D019913:Nuclear Localization Signals / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
chemical_list: D002232:Carbocyanines; D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D050778:NFATC Transcription Factors; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C085321:cyanine dye 5; D049452:Green Fluorescent Proteins