title: Detection of beta-subunit human chorionic gonadotropin mRNA in the peripheral blood of patients with nonmetastatic gestational trophoblastic disease.
abstract: OBJECTIVE
  The aim of this study was to detect beta-subunit human chorionic gonadotropin (beta(h)CG) mRNA in the peripheral blood samples of patients with nonmetastatic gestational trophoblastic disease (GTD) undergoing hysterectomy.
  
  
  METHODS
  Heparinized peripheral blood samples were obtained from four patients with nonmetastatic GTD before, during, and after hysterectomy. The beta(h)CG mRNA expression was examined by reverse transcriptase-polymerase chain reaction using beta(h)CG primers. The expression of beta(h)CG mRNA was quantified using a densitometer.
  
  
  RESULTS
  Beta(h)CG expression was detected in all patients before and during hysterectomy. The expression of beta(h)CG mRNA during operation was so high that it could not be quantified using densitometer. The expression decreased rapidly after operation.
  
  
  CONCLUSIONS
  Disseminated trophoblastic cells are present in the peripheral blood even in cases without metastasis. Trophoblastic cells circulating in the peripheral blood can be reduced by surgical intervention.
authors.affiliation: Department of Obstetrics and Gynecology, Chiba University School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. suzukak@med.m.chiba-u.ac.jp
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Suzuka
authors.forename: Hideo
authors.initials: H
authors.lastname: Matsui
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Iitsuka
authors.forename: Souei
authors.initials: S
authors.lastname: Sekiya
mesh_terms: D000328:Adult; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D007044:Hysterectomy; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000097:blood*; D014328:Trophoblastic Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D014407:Tumor Cells, Cultured
chemical_list: D018997:Chorionic Gonadotropin, beta Subunit, Human; D012333:RNA, Messenger