Cloaking cytolytic peptides for liposome-based detection and potential drug delivery.
Potent cytolytic peptides with specific tethering and cloaking sites have been synthesised and used to release payload from liposomes in a quantitative manner. A functionally located cloaking site has been modified specifically by simple conjugation without adversely affecting the cytolytic properties of the peptide. The cytolytic activity of modified peptides was then efficiently (>98%) cloaked and uncloaked by ligand-protein or hapten-antibody interactions. The principle of a dual response peptide has been demonstrated using an avidin-cloaked pH-sensitive peptide. Biospecific cloaking/uncloaking provided a new sensitive (approximately 12 pmol) homogeneous diagnostic and also appears potentially suited to bioresponsively targeted release of antimicrobial, anticancer and other drugs now delivered using liposomes.
Aojula
Harmesh S
HS
School of Pharmacy and Pharmaceutical Sciences, University of Manchester, UK.
Offerman
Shaun
S
Aojula
Raj R
RR
Hutchinson
Alistair P
AP
Nicklin
Steve
S
Clarke
David J
DJ
D000595:Amino Acid Sequence; D001360:Avidin; D001710:Biotin; D003603:Cytotoxins / Q000008:administration & dosage* / Q000138:chemical synthesis; D016503:Drug Delivery Systems; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D008555:Melitten / Q000008:administration & dosage / Q000138:chemical synthesis; D008969:Molecular Sequence Data; D010455:Peptides / Q000008:administration & dosage* / Q000138:chemical synthesis
D003603:Cytotoxins; D008081:Liposomes; D010455:Peptides; D001360:Avidin; D008555:Melitten; D001710:Biotin