Green fluorescent protein as a visual marker in somatic hybridization.
Using a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression was detected in transgenic citrus protoplasts, hybrid callus, embryos and plants. It is demonstrated that GFP can be used for the continuous monitoring of the fusion process, localization of hybrid colonies and callus, and selection of somatic hybrid embryos and plants.
Olivares-Fuster
O
O
Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Moncada, Spain.
Peña
L
L
Duran-Vila
N
N
Navarro
L
L
D015415:Biomarkers; D002455:Cell Division / Q000235:genetics; D002957:Citrus / Q000196:embryology / Q000235:genetics / Q000254:growth & development; D046508:Culture Techniques; D005434:Flow Cytometry; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins; D006822:Hybrid Cells / Q000166:cytology / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics; D030821:Plants, Genetically Modified; D011523:Protoplasts / Q000166:cytology / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D015415:Biomarkers; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins