Automated PCR/sequence template purification.
A commercially available filtration method is described for the purification of polymerase chain reaction (PCR) templates and sequence-labeled products. The methodology is described for the automation of this application and its use on a high-throughput liquid handling robot and capillary-based automated DNA sequencer. The application provides good-quality DNA, is relatively cheap, and can be used in either 96- or 384-well format.
Clarke
S C
SC
Department of Microbiology, Stobhill Hospital, Glasgow, UK. stuart.clarke@northglasgow.scot.nhs.uk
Diggle
M A
MA
D015373:Bacterial Typing Techniques; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D008851:Micropore Filters; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012371:Robotics; D017422:Sequence Analysis, DNA; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics; D013698:Templates, Genetic