[Studies on cathepsins in Schistosoma japonicum].
OBJECTIVE
To identify the cathepsins in adult worms of Schistosoma japonicum and their proteolytic activities.


METHODS
The vomitus from adult worms of S. japonicum was collected. The proteolytic activity of cathepsins in the vomitus was identified and measured using synthetic substrates with arginyl bonds.


RESULTS
Specific synthetic subtracts Z-Arg-Arg-AMC (for cathepsin B) and Z-Phe-Arg-AMC (for cathepsin B and L) were degraded by the vomitus. An inhibitor, Z-Phe-PheCHN2 for cathepsin L, inhibited partially the activity of the vomitus to degrade Z-Phe-Arg-AMC. The optimal pH for cathepsin B/L is 5.0-5.5.


CONCLUSIONS
The vomitus of S. japonicum has cathepsin B- and L-like activity.
Li
Y
Y
Department of Parasitology, Tongji Medical University, Wuhan 430030.
Fen
Y
Y
Caffrey
C
C
Ruppel
A
A
D000818:Animals; D002403:Cathepsins / Q000032:analysis*; D012549:Schistosoma japonicum / Q000201:enzymology*
D002403:Cathepsins