title: A histochemical study of the regeneration process after injury by pulsed Nd:YAG laser irradiation of root canals.
abstract: Histological changes of rat dental pulp cells were followed after injury. The regeneration process after 3, 6 and 10 days was monitored. Mandibular incisors were irradiated with a pulsed Nd:YAG laser at 2 W and 20 pulses per sec (pps) for 5 sec and the pulp was examined histologically and immunohistochemically for TGF-beta1. Eruption of the developing tooth was disturbed for a short period only. Rapid formation of osteodentin was observed. After 3 days, a zone of fibrodentin matrix as well as newly formed vessels were found. Afterwards, regenerative dentin formation was observed accompanied by the formation of a layer of odontoblast-like cells in the damaged area. Immunohistochemical staining of TGF-beta1 showed that positivity was present in small tissue areas beneath the mantle dentin, the zone of fibrodentin matrix and odontoblast-like cells. These results indicate that pulsed Nd:YAG laser irradiation of rat incisor pulp induces formation of osteodentin, and TGF-beta1 plays a role during regeneration.
authors.affiliation: Department of Endodontics, Showa University School of Dentistry, Tokyo, Japan. mumingg@aol.com
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Murakami
authors.forename: Arine
authors.initials: A
authors.lastname: Unno
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kimura
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Okano
authors.forename: Mozammal
authors.initials: M
authors.lastname: Hossain
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Koukichi
authors.initials: K
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D004396:Coloring Agents; D003782:Dental Pulp / Q000473:pathology / Q000502:physiology / Q000528:radiation effects; D003786:Dental Pulp Cavity / Q000293:injuries* / Q000473:pathology / Q000502:physiology*; D003804:Dentin / Q000378:metabolism; D007150:Immunohistochemistry; D007180:Incisor / Q000293:injuries / Q000473:pathology / Q000502:physiology; D007834:Lasers; D008297:Male; D009804:Odontoblasts / Q000502:physiology; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*; D014078:Tooth Eruption / Q000502:physiology
chemical_list: D004396:Coloring Agents