Nonperturbing fluorescent labeling of polysaccharides.
A fluorescent labeling procedure, which does not perturb macromolecular conformations, was employed to bind a rhodamine derivative to the reducing end of several water-soluble polysaccharides by reductive amination in the presence of sodium cyanoborohydride. Fluorescence correlation spectroscopy, atomic force microscopy, and size exclusion chromatography were used to demonstrate that the conformations of the polysaccharides schizophyllan, polygalacturonic acid (PGUA), succinoglycan, and several dextrans were maintained following the labeling procedure.
Meunier
Françoise
F
CABE (Analytical and Biophysical Environmental Chemistry), Sciences II, 30 Quai Ernest Ansermet, 1211 Geneva 4, Switzerland.
Wilkinson
Kevin J
KJ
D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D003911:Dextrans / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D018625:Microscopy, Atomic Force; D010368:Pectins / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D012235:Rhodamines; D012680:Sensitivity and Specificity; D012566:Sizofiran / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D003911:Dextrans; D005456:Fluorescent Dyes; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D012235:Rhodamines; C056779:succinoglycan; D012566:Sizofiran; D010368:Pectins; C003181:polygalacturonic acid