The ribonuclease protection assay: a powerful tool for the veterinary pathologist.
Veterinary pathologists engaged in basic research use a variety of methods to study disease pathogenesis at the light microscopic and submicroscopic (protein and mRNA) levels. The ribonuclease protection assay is a sensitive and accurate method to measure mRNA expression. The major advantages of the assay are that multiple mRNA species can be measured simultaneously in a single total RNA sample and that the assay has relatively high throughput. The major disadvantage is that the assay requires moderate technical skill.
Rottman
J B
JB
Experimental Pathology, Millennium Pharmaceuticals, Cambridge, MA 02139, USA. jrottman@mpi.com
D000818:Animals; D010336:Pathology / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D012260:Ribonucleases; D014730:Veterinary Medicine / Q000379:methods
D012333:RNA, Messenger; D012260:Ribonucleases