Localization of group IB phospholipase A(2) isoform in the gills of the red sea bream, Pagrus (Chrysophrys) major.
We previously reported that PLA(2) activity in the gills is higher than that in other tissues in red sea bream and purified PLA(2) from the gills belongs to the group IB PLA(2) as well as other red sea bream PLA(2)s. In this study, we reconfirmed that the level of PLA(2) activity is extremely high in the gills compared with other tissues, and gill PLA(2) was detected only in the gills by immunoblotting and inhibition test using anti-gill PLA(2) monoclonal antibody. The level of PLA(2) activity and protein expression in the gills are well correlated. Fish can be roughly divided into high and low groups based on the level of PLA(2) activity. Gill PLA(2) was detected in the gills of the high group, but not the low group by immunoblotting. In the gills of the high group, gill PLA(2) was detected in the mucous cells and pavement cells located on the surface of gill epithelia by immunohistochemistry. On the other hand, positive signals were observed only in the mucous cells by in situ hybridization. We also isolated inactive proPLA(2), having AR propeptide, preceding the mature enzyme from the gill extract. These results suggest that gill PLA(2) is synthesized as an inactive proPLA(2) in the mucous cells and is secreted to the surface of gill epithelia.
Uchiyama
S
S
Laboratory of Molecular Cell Biology, Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashihiroshima, 739-8528, Japan.
Fujikawa
Y
Y
Uematsu
K
K
Matsuda
H
H
Aida
S
S
Iijima
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D005880:Gills / Q000201:enzymology*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D010741:Phospholipases A / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D021541:Sea Bream
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D010741:Phospholipases A