[Application of dot immunogold filtration assay for diagnosis of Schistosomiasis japonica].
OBJECTIVE
To quest for a simple and convenient assay for the diagnosis of schistosomiasis.


METHODS
Three monoclonal antibodies against different epitopes of antigens of S. japonicum were used to develop a dot immunogold filtration assay for detection of schistosomal antigen-antibody reaction on the membrane during the filtration process. The results could be read with the naked eye within a few minutes.


RESULTS
The sensitivity of the SEA assay was 16 ng/ml. In 69 serum samples from patients with chronic schistosomiasis, the positive rate and specificity of this assay were 60.8% and 95.2%, respectively. Dot-ELISA was used in detecting 137 serum samples from patients with chronic schistosomiasis, the positive rate and specificity were 54.7% and 94.6%, respectively. When sandwich-ELISA was used in detecting 118 serum samples from patients with chronic schistosomiasis, the positive rate and specificity were 61.9% and 95.7%, respectively.


CONCLUSIONS
The sensitivity and specificity of DIFA were similar to those of ELISA, but DIFA required less time and no special equipment.
Zhang
E
E
Department of Parasitology, Shanghai Second Medical University, Shanghai 200025.
Wu
C
C
Shen
D
D
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D012554:Schistosomiasis japonica / Q000175:diagnosis*