title: Matrilysin (matrix metalloproteinase-7) expression in human junctional epithelium.
abstract: Matrilysin is a matrix metalloproteinase expressed in exocrine and mucosal epithelium in many human tissues. Immunohistochemical staining showed that matrilysin is expressed in suprabasal cells of junctional epithelium facing the teeth and in epithelial cell rests of Malassez. No matrilysin expression was seen in the periodontal pocket tissue. In a tissue culture model mimicking junctional epithelium, matrilysin expression was also observed in suprabasal epithelial cells. Of 13 anaerobic oral bacterial species tested, F. nucleatum, F. necrophorum, P. endodontalis, and P. denticola stimulated matrilysin expression in porcine periodontal ligament epithelial cells from 2.5- to 5.7-fold, compared with untreated cells. The enzyme was localized in intracytoplasmic vesicles that also reacted with antibodies against lysosomal membrane protein h-lamp-1. The results indicate that matrilysin may play an important role in the normal physiology of junctional epithelium.
authors.affiliation: Department of Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, 2199 Wesbrook mall, Vancouver, V6T 1Z3, Canada. jukka.uitto@helsinki.fi
authors.forename: V-J
authors.initials: VJ
authors.lastname: Uitto
authors.forename: J I
authors.initials: JI
authors.lastname: Salonen
authors.forename: J D
authors.initials: JD
authors.lastname: Firth
authors.forename: H
authors.initials: H
authors.lastname: Jousimies-Somer
authors.forename: U
authors.initials: U
authors.lastname: Saarialho-Kere
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001421:Bacteria, Anaerobic / Q000276:immunology; D015152:Blotting, Northern; D002478:Cells, Cultured; D022162:Cytoplasmic Vesicles / Q000201:enzymology; D004846:Epithelial Attachment / Q000201:enzymology*; D004847:Epithelial Cells / Q000201:enzymology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000096:biosynthesis*; D009061:Mouth Mucosa / Q000201:enzymology; D010513:Periodontal Ligament / Q000166:cytology / Q000201:enzymology*; D013552:Swine; D015854:Up-Regulation
chemical_list: D020783:Matrix Metalloproteinase 7