Promoter II of the bovine acetyl-coenzyme A carboxylase-alpha-encoding gene is widely expressed and strongly active in different cells.
Three promoters express the bovine gene encoding the enzyme acetyl-CoA carboxylase-alpha (ACC alpha) known to be rate-limiting for fatty acid synthesis. Our sequence of promoter II shows that PII is evolutionary conserved, unlike PI or PIII. In vivo expression of PII reveals little tissue-specific restrictions. The proximal 133 bp of this promoter are sufficient for a strong basal expression in different cell lines.
Mao
Jianqiang
J
Research Institute for the Biology of Farm Animals, Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, Germany.
Seyfert
Hans-Martin
HM
D000103:Acetyl-CoA Carboxylase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D005091:Exons / Q000235:genetics; D017930:Genes, Reporter; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D016415:Sequence Alignment; D014018:Tissue Distribution
D000103:Acetyl-CoA Carboxylase