Microimmunoassay using a protein chip: optimizing conditions for protein immobilization.
Optimizing conditions for the microarraying of protein antigens onto glass slides were studied. Various vendors, surface functional groups, buffers, and fixatives were evaluated to enhance protein binding. A total of 125 pg of human immunoglobulin was detectable with this assay system, suggesting that protein microarray can be applied for routine immunodiagnosis.
Seong
Seung-Yong
SY
Department of Microbiology and Immunology, Seoul National University College of Medicine and Institute of Endemic Disease, Seoul National University Medical Research Center, Chongno-gu, Seoul 110-799, Republic of Korea. sseong@niaid.nih.gov
D000941:Antigens / Q000276:immunology; D019921:Biotinylation; D005455:Fluorescent Antibody Technique; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000276:immunology; D007202:Indicators and Reagents; D011506:Proteins / Q000276:immunology; D011933:Reagent Kits, Diagnostic
D000941:Antigens; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D011506:Proteins; D011933:Reagent Kits, Diagnostic