[Total antioxidant activity of the lacrimal fluid].
Total antioxidant activity of the lacrimal fluid (TAALF) was studied by luminol-dependent iron-induced chemiluminescence in an original model system on citrate phosphate buffer. Total antioxidant activity was estimated in percent of control (light sum of the model system fluorescence). The mean decrease of the light sum of the model system fluorescence in the presence of normal human lacrimal fluid supernatant was more than 50%, in glaucoma 67.2 +/- 4.43%, in cataract 58.5 +/- 2.61%, and in uveitis 76.9 +/- 1.13% of control. Traditional therapy of uveitis was associated with an increase of TAALF. Use of the proposed method for TAALF evaluation together with clinical data allows justified and individual antioxidant treatment of patients with ocular diseases and helps monitor the treatment efficiency. The study is noninvasive, cheap, and simple, which recommends evaluation of TAALF in clinical practice as a rapid test indicating the level of free radical oxidation in ocular tissues.
Semes'ko
S G
SG
Farkhutdinov
R R
RR
D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol; D012680:Sensitivity and Specificity; D013666:Tears / Q000737:chemistry* / Q000378:metabolism
D000975:Antioxidants; D005609:Free Radicals; D008165:Luminol