Amiodarone induces apoptosis in L-132 human lung epithelial cell line.
To investigate whether amiodarone induces apoptosis in cells of the L-132 human lung epithelial cell line, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, 4,6-diamidino-2-phenylindole staining, DNA fragmentation assay, reverse transcription-polymerase chain reaction, and casapse-3 enzyme assay were performed. Through morphological and biochemical analyses, it was demonstrated that L-132 cells treated with amiodarone exhibit several features of apoptosis. In addition, it was shown that amiodarone increases the mRNA levels of bax and caspase-3. Based on the results, amiodarone appears to activate specific intracellular death-related pathways, including possibly the bax-dependent caspase-3 activation pathway, and thus induce apoptosis in human lung epithelial cells.
Choi
In-Serk
IS
Department of General Thorax and Cardiovascular Surgery, College of Medicine, Kyung Hee University, Seoul, South Korea.
Kim
Bum-Shik
BS
Cho
Kyu-Seok
KS
Park
Joo-Chul
JC
Jang
Mi-Hyeon
MH
Shin
Min-Chul
MC
Jung
Sae-Bin
SB
Chung
Joo-Ho
JH
Kim
Chang-Ju
CJ
D000638:Amiodarone / Q000633:toxicity*; D000889:Anti-Arrhythmia Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D004396:Coloring Agents; D053938:DNA Fragmentation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D007202:Indicators and Reagents; D008168:Lung / Q000166:cytology* / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts; D013844:Thiazoles
D000889:Anti-Arrhythmia Agents; D004396:Coloring Agents; D007202:Indicators and Reagents; D012333:RNA, Messenger; D013778:Tetrazolium Salts; D013844:Thiazoles; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; C022616:thiazolyl blue; D000638:Amiodarone