The gene expression and deficiency phenotypes of Cockayne syndrome B protein in Caenorhabditis elegans.
The Caenorhabditis elegans Cockayne syndrome B protein homologue is encoded by 10 exons of the predicted open reading frame F53H4.1. The gene is expressed in germ cells and all somatic cells of the embryonic to adult stage. Although the gene expression was ubiquitous, its expression level was relatively higher in dividing cells and cells that play fundamental roles in essential physiological functions such as feeding, sensation, and reproduction. RNA interference of the gene hypersensitized C. elegans to UV radiation, as observed in enhanced germ cell proliferation arrest and apoptosis, and increased embryonic lethality, suggesting its role in nucleotide excision repair.
Lee
Myon Hee
MH
Department of Biochemistry, College of Science, Yonsei University, Seoul 120-749, South Korea.
Ahn
Byungchan
B
Choi
In Soon
IS
Koo
Hyeon-Sook
HS
D000818:Animals; D017173:Caenorhabditis elegans; D003057:Cockayne Syndrome / Q000235:genetics*; D004265:DNA Helicases / Q000235:genetics*; D004260:DNA Repair; D045643:DNA Repair Enzymes; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D010641:Phenotype; D000075223:Poly-ADP-Ribose Binding Proteins; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D022661:RNA, Untranslated; D011993:Recombinant Fusion Proteins / Q000235:genetics
D000075223:Poly-ADP-Ribose Binding Proteins; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D022661:RNA, Untranslated; D011993:Recombinant Fusion Proteins; D004265:DNA Helicases; C078673:ERCC6 protein, human; D045643:DNA Repair Enzymes