Transient modifications of respiratory capacity in thymic cells during murine radioleukemogenesis.
The evolution of mitochondrial oxidative phosphorylation was studied during cancer induction in a model of thymic radiolymphomagenesis in C57BL/Ka mice. During the preneoplastic period, thymuses displayed an increase of the cytochrome c oxidase activity and oxygen consumption together with oxidative DNA damage assessed by the presence of the 8-hydroxydeoxyguanine DNA base modification. These transient changes in mitochondrial functional activity were not observed in thymuses of mice rescued from lymphoma development by a bone marrow graft, suggesting an important role of mitochondria for neoplastic transformation in this model, which might therefore be of interest to test the utilization of antioxidants for the prevention of radiation-induced malignancies.
Verlaet
Myriam
M
Laboratory of Pathological Anatomy and Cytology, University of Liège, Liège, Belgium. m.verlaet@ulg.ac.be
Duyckaerts
Claire
C
Rahmouni
Souad
S
Denis
Ghislaine
G
Humblet
Chantal
C
Greimers
Roland
R
Sluse
Francis E
FE
Boniver
Jacques
J
Defresne
Marie-Paule
MP
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D016026:Bone Marrow Transplantation; D019069:Cell Respiration; D002471:Cell Transformation, Neoplastic; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D017403:In Situ Hybridization; D007953:Leukemia, Radiation-Induced / Q000378:metabolism*; D008223:Lymphoma / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress / Q000502:physiology; D010101:Oxygen Consumption; D011289:Preleukemia / Q000378:metabolism; D013950:Thymus Gland / Q000528:radiation effects; D013953:Thymus Neoplasms / Q000378:metabolism*; D015854:Up-Regulation; D014916:Whole-Body Irradiation
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003576:Electron Transport Complex IV; D003849:Deoxyguanosine