Corticosterone administration to rat pups, but not maternal separation, affects sexual maturation and glucocorticoid receptor immunoreactivity in the testis.
Prenatal stress strongly affects sexual dimorphism of male rats. Much less information is instead available on the effects of postnatal stress on sexual maturation during the so-called stress hyporesponsive period (SHRP). For this reason, we compared corticosterone-treated (CS; 10 mg/kg sc, suspended in sesame oil) or maternally separated pups (MS; 5 h/day in the first week of life) with control rats. Control and MS pups also received sesame oil injections. The effects of these procedures on physical development (body weight and eye opening), sexual maturation [anogenital distance, testis weight, 3beta-hydroxysteroid dehydrogenase/(Delta5-4) (3betaHSD) isomerase activity and time to testis descent] and glucocorticoid receptor (GR) immunoreactivity in the testis were examined. Corticosterone treatment significantly (P<.05) advanced testis descent and increased testis weight and 3betaHSD activity at puberty. In addition, adult CS rats presented higher levels of GR immunoreactivity in testicular tubules when compared to control and MS rats. No differences were found between control and MS rats. On this basis, we propose that the silencing of adrenocortical function during the SHRP could be finalized to preserve sexual maturation from the influence of glucocorticoid effects. As SHRP is unique to rodents, this phenomenon could be related to their successful reproductive strategy.
Biagini
Giuseppe
G
Department of Biomedical Sciences, Section of Physiology, and Centre of Clinical Nutrition and Metabolic Diseases, University of Modena and Reggio Emilia, Modena, Italy. gbiagini@unimo.it
Pich
Emilio Merlo
EM
D000818:Animals; D000831:Animals, Newborn; D003345:Corticosterone / Q000097:blood / Q000494:pharmacology*; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008426:Maternal Deprivation; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D011965:Receptors, Glucocorticoid / Q000032:analysis*; D012741:Sexual Maturation / Q000187:drug effects* / Q000502:physiology; D013737:Testis / Q000737:chemistry* / Q000187:drug effects
D011965:Receptors, Glucocorticoid; D003345:Corticosterone