Estrogen induces elevation of cAMP-dependent protein kinase activity in immortalized hippocampal neurons: imaging in living cells.
17beta-estradiol-stimulated PKA activation in living differentiated H19-7 cells was visualized using DR2, a cell-permeable fluorescent PKA substrate. Estradiol induced marked changes of fluorescence in cytosolic areas and these were inhibited by a PKA inhibitor.
Shingo
A S
AS
Hyogo University School of Health Sciences, Japan. sheala@mcn.ne.jp
Kito
S
S
D000818:Animals; D002460:Cell Line; D017868:Cyclic AMP-Dependent Protein Kinases / Q000096:biosynthesis*; D004790:Enzyme Induction; D004958:Estradiol / Q000494:pharmacology*; D006624:Hippocampus / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D051381:Rats
D004958:Estradiol; D017868:Cyclic AMP-Dependent Protein Kinases