Osteopenia and male-specific sudden cardiac death in mice lacking a zinc transporter gene, Znt5.
We isolated a mammalian gene whose expression transiently increased in response to intimal denudation of rabbit aorta. It was identical to a gene encoding a zinc transporter, ZNT5, reported very recently by others. Mice deficient for this gene showed poor growth and a decrease in bone density due to impairment of osteoblast maturation to osteocyte. More than 60% of male null mice died suddenly because of the bradyarrhythmias. Analysis of gene-expression profiles in murine hearts by means of an oligonucleotide microarray disclosed that a subset of genes encoding immediate-early response factors (IEGs) and heat shock proteins (HSPs) were down-regulated in Znt5-null mice. These results indicate that Znt5 protein plays an important role in maturation of osteoblasts and in maintenance of the cells involved in the cardiac conduction system, partly owing to dysregulated expression of IEGs and HSPs.
Inoue
Koichi
K
Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, University of Tokyo, Japan.
Matsuda
Koichi
K
Itoh
Makoto
M
Kawaguchi
Hiroshi
H
Tomoike
Hitonobu
H
Aoyagi
Teruhiko
T
Nagai
Ryozo
R
Hori
Masatsugu
M
Nakamura
Yusuke
Y
Tanaka
Toshihiro
T
D000595:Amino Acid Sequence; D000818:Animals; D001851:Bone Diseases, Metabolic / Q000235:genetics*; D027682:Cation Transport Proteins; D016757:Death, Sudden, Cardiac; D020869:Gene Expression Profiling; D006329:Heart Conduction System / Q000166:cytology; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology; D011817:Rabbits
D027682:Cation Transport Proteins; D026901:Membrane Transport Proteins; C486753:Slc30a5 protein, mouse