title: Temporal and subcellular localization of PR-1 proteins in tomato stem tissues infected by virulent and avirulent isolates of Phytophthora capsici.
abstract: Immunoblot analysis and immunogold labeling of PR-1 protein (pathogenesis-related protein 1) in tomato (Lycopersicon esculentum Mill.) were performed to examine the temporal and spatial expression patterns of PR-1 protein induced by Phytophthora capsici infection. Soluble proteins with molecular masses of 10, 17, 25, 27 and 75 kDa were induced and accumulated in P. capsici-infected stem tissues during the compatible and incompatible interactions. Western blot analysis revealed that expression of PR-1 protein (17 kDa), at 12 to 24 h after inoculation, occurred earlier in the incompatible than in the compatible interaction. Immunogold labeling of PR-1 proteins occurred over cell walls and cytoplasm of the host and the oomycete pathogen and at the interface between host and oomycete cell walls at 24 h after inoculation in the compatible interaction. In the incompatible interaction, numerous PR-1 proteins accumulated predominantly over oomycete cell walls and at the interface between host and oomycete cell walls. The quantity of PR-1 proteins deposited in both host and oomycete cells was much less in the compatible than the incompatible interaction. Healthy tomato stem tissue was nearly free of immunogold labeling of PR-1 proteins.
authors.affiliation: Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University, Seoul.
authors.forename: Jeum Kyu
authors.initials: JK
authors.lastname: Hong
authors.forename: Byung Kook
authors.initials: BK
authors.lastname: Hwang
mesh_terms: D015153:Blotting, Western / Q000379:methods; D018506:Gene Expression Regulation, Plant; D007150:Immunohistochemistry; D018551:Solanum lycopersicum / Q000737:chemistry / Q000166:cytology / Q000382:microbiology* / Q000648:ultrastructure; D017348:Microscopy, Electron, Scanning Transmission; D010838:Phytophthora / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000032:analysis* / Q000096:biosynthesis; D018547:Plant Stems / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods; D013347:Subcellular Fractions / Q000378:metabolism / Q000382:microbiology; D014774:Virulence
chemical_list: D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant