title: Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies.
abstract: Autoantibodies are often detected in hepatocellular carcinoma (HCC), and these responses may represent recognition of tumor Ags that are associated with transformation events. The identities of these Ags, however, are less well known. Using serological analysis of recombinant cDNA expression libraries (SEREX) from four HCC patients, we identified 55 independent cDNA sequences potentially encoding HCC tumor Ags. Of these genes, 15 are novel. Two such proteins, HCA587 and HCA661, were predominantly detected in testis, but not in other normal tissues, except for a weak expression in normal pancreas. In addition to HCC, these two Ags can be found in cancers of other histological types. Therefore, they can be categorized as cancer-testis (CT) Ags. Two other Ags (HCA519 and HCA90) were highly overexpressed in HCC and also expressed in cancer cell lines of lung, prostate, and pancreas, but not in the respective normal tissues. Four other Ags were identified to be expressed in particular types of cancer cell lines (HCA520 in an ovarian cancer cell line, HCA59 and HCA67 in a colon cancer cell line, HCA58 in colon and ovarian cancer cell lines), but not in the normal tissue counterpart(s). In addition, abundant expression of complement inactivation factors was found in HCC. These results indicate a broad range expression of autoantigens in HCC patients. Our findings open an avenue for the study of autoantigens in the transformation, metastasis, and immune evasion in HCC.
authors.affiliation: Immunology Department, School of Basic Medical Science, Peking University Health Science Center, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Ke-Jun
authors.initials: KJ
authors.lastname: Han
authors.forename: Xue-Wen
authors.initials: XW
authors.lastname: Pang
authors.forename: Hilary A
authors.initials: HA
authors.lastname: Vaughan
authors.forename: Wei
authors.initials: W
authors.lastname: Qu
authors.forename: Xue-Yuan
authors.initials: XY
authors.lastname: Dong
authors.forename: Ji-Run
authors.initials: JR
authors.lastname: Peng
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Zhao
authors.forename: Jing-An
authors.initials: JA
authors.lastname: Rui
authors.forename: Xi-Sheng
authors.initials: XS
authors.lastname: Leng
authors.forename: Jonathan
authors.initials: J
authors.lastname: Cebon
authors.forename: Antony W
authors.initials: AW
authors.lastname: Burgess
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Chen
mesh_terms: D000368:Aged; D000912:Antibodies, Neoplasm / Q000032:analysis*; D000937:Antigen-Antibody Reactions / Q000235:genetics; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis*; D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018797:Cell Cycle Proteins; D018076:DNA, Complementary / Q000096:biosynthesis; D026261:Diazepam Binding Inhibitor / Q000032:analysis / Q000276:immunology; D015723:Gene Library; D006801:Humans; D007519:Isoantigens / Q000032:analysis / Q000235:genetics / Q000276:immunology; D008113:Liver Neoplasms / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000276:immunology; D009928:Organ Specificity / Q000235:genetics / Q000276:immunology; D017384:Sequence Deletion / Q000276:immunology; D050680:Transcription Factor DP1; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D000912:Antibodies, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D001323:Autoantibodies; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D026261:Diazepam Binding Inhibitor; D007519:Isoantigens; D050680:Transcription Factor DP1; D014157:Transcription Factors