title: Immortalization of normal adult human middle ear epithelial cells using a retrovirus containing the E6/E7 genes of human papillomavirus type 16.
abstract: A human middle ear epithelial cell line (HMEEC-1) was established using human papillomavirus E6/E7 genes. HMEEC-1 has remained morphologically and phenotypically stable, even after 50 passages. The cells are anchorage-dependent and nontumorigenic when injected into nude mice. This cell line thus provides a new tool for the study of normal cell biology and the pathological processes associated with the epithelial cells of the middle ear in otitis media. HMEEC-1 will also be useful in the search for new drugs and biological agents for the treatment of otitis media.
authors.affiliation: Gonda Department of Cell and Molecular Biology, Los Angeles, California, USA.
authors.forename: Young-Myoung
authors.initials: YM
authors.lastname: Chun
authors.forename: Sung-Kyun
authors.initials: SK
authors.lastname: Moon
authors.forename: Haa-Yung
authors.initials: HY
authors.lastname: Lee
authors.forename: Paul
authors.initials: P
authors.lastname: Webster
authors.forename: Derald E
authors.initials: DE
authors.lastname: Brackmann
authors.forename: Johng S
authors.initials: JS
authors.lastname: Rhim
authors.forename: David J
authors.initials: DJ
authors.lastname: Lim
mesh_terms: D000328:Adult; D000818:Animals; D002460:Cell Line; D004432:Ear, Middle / Q000166:cytology*; D004847:Epithelial Cells; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16