[Genetic relationships among Oxya agavisa and other relative species revealed by Cyt b sequences].
The mtDNA Cyt b genes (432 bp) were sequenced for 16 individuals of Oxya agavisa from 7 Chinese region, 3 other relative species (O. japonica, O. chinensis and O. intricata), and two outgroup species (Pseudomorphacris hollisi and Tetrix japonica). In the obtained sequences of O. agavisa, A% + T% was about 71.0% and 9 polymorphic nucleotide sites were observed (about 2.08%), among which no transversions were observed and only one substitution resulted in the variation of amino acid. In the case of 3 other species of Oxya, A% + T% was about 72.5% (O. chinensis) and 70% (O. japonica and O. intricata), respectively. A% + T% in the third site was much higher than the other two sites in all the analyzed Oxya species (O. agavisa, 88.3%; O. chinensis, 92.4%; O. japonica, 86.8%; O. intricata, 89.5%). Six haplotypes of O. agavisa were compared with the 3 other Oxya species and 73 polymorphic nucleotide sites were examined (about 16.9%) in all. Phylogenetic tree was constructed with the Neighbor-Joining methods using P. hollisi and T. japonica as outgroups and the confidence of nodes in the tree was evaluated by Bootstrap(1000 replicates). The NJ tree showed that different haplotypes of O. agavisa clustered together, but no clear relationships between haplotypes and the geographic regions would be inferred. The population from Mount Wuyi belongs to the peculiar group and is relatively distant to other populations. In addition, the NJ tree showed that O. agavisa was closer to O. japonica and O. chinensis closer to O. intricata, which was different from the previous results of morphologic, chromosome karyotype and bands. The relationships among different species of Oxya still need to be further clarified.
Ren
Zhu-Mei
ZM
College of Life Science and Technology, Shanxi University, Taiyuan 030006, China. zmren@sxu.edu.cn
Ma
En-Bo
EB
Guo
Ya-Ping
YP
D000818:Animals; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics*; D018076:DNA, Complementary; D004272:DNA, Mitochondrial / Q000032:analysis; D006110:Grasshoppers / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D003573:Cytochrome b Group; D018076:DNA, Complementary; D004272:DNA, Mitochondrial