Effects of major histocompatibility complex class II knockout on mouse bone mechanical properties during development.
We investigated the effect of major histocompatibility complex class II (MHC II) knockout on the development of the mouse peripheral skeleton. These C2D mice had less skeletal development at 8, 12 and 16 weeks of age compared to wild-type C57BL/6J (B6) male mice. The C2D mice had decreased femur mechanical, geometric and compositional measurements compared to wild type mice at each of these ages. C2D femur stiffness (S), peak force in 3-pt bending (Pm), and mineral mass (Min-M) were 74%, 64% and 66%, respectively, of corresponding B6 values at 8 weeks of age. Similar differences were measured at 12 weeks (for which C2D femoral S, Pm and Min-M were 71%, 72% and 73%, respectively, of corresponding B6 values) and at 16 weeks (for which C2D femoral S, Pm and Min-M were 80%, 66% and 61%, respectively, of corresponding B6 values). MHC II knockout delays the development of adult bone properties and is accompanied by lower body mass compared to wild-type controls.
Simske
Steven J
SJ
Department of Aerospace Engineering Sciences, Univ. Colorado, Campus Box 429, Boulder, CO 80309, USA.
Bateman
Ted A
TA
Smith
Erin E
EE
Ferguson
Virginia L
VL
Chapes
Stephen K
SK
D000818:Animals; D015519:Bone Density / Q000502:physiology; D001846:Bone Development / Q000235:genetics* / Q000502:physiology; D003345:Corticosterone / Q000097:blood; D005269:Femur / Q000254:growth & development; D005802:Genes, MHC Class II / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
D003345:Corticosterone