title: The effect of nonsteroidal antiinflammatory drugs ibuprofen, flurbiprofen, and diclofenac on in vitro and in vivo growth of mouse fibrosarcoma.
abstract: For suppression of primary G:5:113 fibrosarcoma growth, three structurally different cyclooxygenase (COX) inhibitors (ibuprofen, flurbiprofen, and diclofenac) were administered intraperitoneally (i.p.) in two regimens starting on day 5 after tumor-cell inoculation. Repeated application of 0.15 mg/mouse/day during 14 consecutive days significantly suppressed the tumor growth and increased the percentage of surviving mice. Similar tendency, however without significant differences, was observed when animals were given 0.5 mg/day for five consecutive days. These results suggest that a time schedule of drug application is important for the therapeutic effect. Suppressive effect of diclofenac and flurbiprofen on tumor growth was also observed under in vitro conditions. We conclude that suppressive effect of these drugs on tumor growth in vivo comprises both direct effects of COX inhibitors on fibrosarcoma cells and indirect effects that are presumably mediated by extratumoral sources. Our findings encourage the use of COX inhibitors in the therapy of fibrosarcoma.
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Hoferová
authors.forename: Peter
authors.initials: P
authors.lastname: Fedorocko
authors.forename: Jirina
authors.initials: J
authors.lastname: Hofmanová
authors.forename: Michal
authors.initials: M
authors.lastname: Hofer
authors.forename: Vladimír
authors.initials: V
authors.lastname: Znojil
authors.forename: Katerina
authors.initials: K
authors.lastname: Minksová
authors.forename: Karel
authors.initials: K
authors.lastname: Soucek
authors.forename: Alena
authors.initials: A
authors.lastname: Egyed
authors.forename: Alois
authors.initials: A
authors.lastname: Kozubík
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004008:Diclofenac / Q000627:therapeutic use*; D005354:Fibrosarcoma / Q000188:drug therapy* / Q000473:pathology; D005480:Flurbiprofen / Q000627:therapeutic use*; D007052:Ibuprofen / Q000627:therapeutic use*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009374:Neoplasms, Experimental; D015996:Survival Rate; D014407:Tumor Cells, Cultured / Q000187:drug effects*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004008:Diclofenac; D005480:Flurbiprofen; D007052:Ibuprofen