Immunohistochemical study of synphilin-1 in brains of patients with dementia with Lewy bodies - synphilin-1 is non-specifically implicated in the formation of different neuronal cytoskeletal inclusions.
Dementia with Lewy bodies brains were immunohistochemically investigated using anti-synphilin-1 antibodies. The alpha-synuclein-positive brainstem type and well-defined cortical type Lewy bodies (LB) were positive for synphilin-1, while ill-defined LB and LB-related neurites were negative, suggesting that synphilin-1 does not directly associate with alpha-synuclein. Synphilin-1-positive LB were double-positive for phosphorylated neurofilament. In addition, tau-positive neurofibrillary tangles (NFT) were positive for synphilin-1, while neuropil threads were negative. Immunoelectron microscopically, synphilin-1 was located on filamentous components in cortical type LB and on paired helical filaments in NFT. It is likely that synphilin-1 accumulates in the cell body according to the axonal transport blockage, and associates with abnormal cytoskeltons during the formation of LB or NFT, suggesting that synphilin-1 is non-specifically implicated in the formation of different neuronal cytoskeletal inclusions.
Iseki
Eizo
E
Department of Psychiatry, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan. iseki@med.yokohama-cu.yokohama-cu.ac.jp
Takayama
Noriko
N
Furukawa
Yoshiko
Y
Marui
Wami
W
Nakai
Toshiki
T
Miura
Satoshi
S
Uéda
Kenji
K
Kosaka
Kenji
K
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000378:metabolism; D016631:Lewy Bodies / Q000473:pathology* / Q000648:ultrastructure; D020961:Lewy Body Disease / Q000378:metabolism* / Q000473:pathology; D016253:Microscopy, Immunoelectron; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D016501:Neurites / Q000473:pathology* / Q000648:ultrastructure; D016874:Neurofibrillary Tangles / Q000378:metabolism / Q000648:ultrastructure; D016900:Neurofilament Proteins / Q000378:metabolism; D019600:Neuropil Threads / Q000378:metabolism; D010766:Phosphorylation; D016875:tau Proteins / Q000378:metabolism
D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C118958:SNCAIP protein, human; D016875:tau Proteins