Generation of mice deficient for macrophage galactose- and N-acetylgalactosamine-specific lectin: limited role in lymphoid and erythroid homeostasis and evidence for multiple lectins.
Macrophage receptors function in pattern recognition for the induction of innate immunity, in cellular communication to mediate the regulation of adaptive immune responses, and in the clearance of some glycosylated cells or glycoproteins from the circulation. They also function in homeostasis by initiating the engulfment of apoptotic cells. Evidence has suggested that macrophage receptors function to recognize cells that are destined for programmed cell death but not yet overtly apoptotic. We have examined the function of a macrophage receptor specific for unsialylated glycoproteins, known as the mouse macrophage galactose- and N-acetylgalactosamine-specific lectin (mMGL) (Ii et al., J. Biol. Chem. 265:11295-11298, 1990; Sato et al., J. Biochem. [Tokyo] 111:331-336, 1992; Yamamoto et al., Biochemistry 33:8159-8166, 1994). With targeted disruption, we tested whether mMGL is necessary for macrophage function, controlled thymic development, the loss of activated CD8 T cells, and the turnover of red blood cells. Evidence indicates that mMGL may play a nonessential role in several of these macrophage functions. Experiments are presented that indicate the existence of another galactose- and N-acetylgalactosamine-recognizing lectin distinct from mMGL. This may explain the absence of a strong phenotype in mMGL-deficient mice.
Onami
Thandi M
TM
Molecular Biology Section, Division of Biology, Cancer Center, University of California-San Diego, La Jolla, CA 92093, USA.
Lin
Meei-Yun
MY
Page
Dawne M
DM
Reynolds
Shirley A
SA
Katayama
Carol D
CD
Marth
Jamey D
JD
Irimura
Tatsuro
T
Varki
Ajit
A
Varki
Nissi
N
Hedrick
Stephen M
SM
D000818:Animals; D001212:Asialoglycoproteins; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology; D004905:Erythrocyte Aging; D004906:Erythrocyte Count; D004920:Erythropoiesis / Q000235:genetics / Q000502:physiology; D005260:Female; D018390:Gene Targeting; D005816:Genetic Complementation Test; D006410:Hematopoiesis / Q000235:genetics / Q000502:physiology*; D006706:Homeostasis; D037102:Lectins / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D037181:Lectins, C-Type; D008221:Lymphoid Tissue / Q000254:growth & development; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D012799:Sialyltransferases / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D014018:Tissue Distribution; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
D001212:Asialoglycoproteins; D002352:Carrier Proteins; C064720:Clec10a protein, mouse; D037102:Lectins; D037181:Lectins, C-Type; D008565:Membrane Proteins; D012799:Sialyltransferases; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase