Use of the sensititre colorimetric microdilution panel for antifungal susceptibility testing of dermatophytes.
The Sensititre YeastOne antifungal panel was used to test 49 dermatophytes belonging to the species Epidermophyton floccosum, Microsporum gypseum, Microsporum canis, Trichophyton tonsurans, Trichophyton rubrum, and Trichophyton mentagrophytes. The MICs of four antifungals obtained with the Sensititre YeastOne antifungal panel were compared with those obtained by the reference NCCLS microdilution method. The levels of agreement between the two methods (<or=2 dilutions) were 81.6% with amphotericin B, 87.7% with itraconazole, 67.3% with fluconazole, and 69.4% with ketoconazole.
Pujol
I
I
Laboratori de Microbiologia, Hospital Universitari de Sant Joan de Reus. Unitat de Microbiologia, Facultat de Medicina. Institut d'Estudis Avançats, Universitat Rovira i Virgili, Reus, Tarragona, Spain.
Capilla
J
J
Fernández-Torres
B
B
Ortoneda
M
M
Guarro
J
J
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D003883:Arthrodermataceae / Q000187:drug effects*; D003124:Colorimetry; D004396:Coloring Agents; D003881:Dermatomycoses / Q000188:drug therapy / Q000382:microbiology; D025141:Drug Resistance, Fungal; D004821:Epidermophyton / Q000187:drug effects; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D007654:Ketoconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000706:statistics & numerical data; D008864:Microsporum / Q000187:drug effects; D010078:Oxazines; D012680:Sensitivity and Specificity; D014249:Trichophyton / Q000187:drug effects; D014966:Xanthenes
D000935:Antifungal Agents; D004396:Coloring Agents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D017964:Itraconazole; D000666:Amphotericin B; D015725:Fluconazole; D007654:Ketoconazole