[Electrophoretic analysis of soluble antigens of a pathogenic isolate of Trichomonas vaginalis].
OBJECTIVE
To analyze the parasite soluble antigens of a pathogenic isolate of Trichomonas vaginalis.


METHODS
The soluble antigens of a pathogenic isolate of T. vaginalis isolated from a patient suffered from trichomonad vaginitis was studied by means of SDS-PAGE followed by densitometric analysis and immunoblotting.


RESULTS
A total of 14 distinct protein bands was demonstrated using a 10% resolution gel concentration. Densitometrogram indicated that the soluble antigens with MWs of equal to and below 72 kDa accounted for 89.5% of the total volume with the dominant fractions of 48-51 kDa which accounted for 19.2%. Having MWs over 100 kDa accounted only for 7.4%, while the lowest M. W. fraction demonstrated was found to be at the site of 13 kDa. By immunoblotting, the specific anti-T. vaginalis antibodies raised in mice recognized a broad spectrum of molecular size range of these antigenic fraction. However, no immunological reaction was seen at the 86 kDa site.


CONCLUSIONS
The proteins of MWs 48-51 kDa possess a larger protein content and a higher immunological reactivity.
Zhang
Y
Y
Medical College of Yangzhou University, Yangzhou 225001.
Wang
Z
Z
Wu
L
L
Zhou
X
X
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008970:Molecular Weight; D014247:Trichomonas Vaginitis / Q000469:parasitology; D014246:Trichomonas vaginalis / Q000276:immunology* / Q000302:isolation & purification
D000953:Antigens, Protozoan