Establishment of a reproducible tissue culture system for the induction of Arabidopsis somatic embryos.
Somatic embryogenesis is an example of totipotency and is used as a model system for studying embryogenesis. A reproducible tissue culture system was established for the large-scale induction of Arabidopsis somatic embryos. The method allows maintenance of high embryogenic competence over a one-year period. Using this tissue culture system, the expression of embryo-specific genes (ABI3, LEC1, FUS3) was detected in embryogenic cells and somatic embryos. Exogenous application of abscisic acid enhanced the expression of some late-embryogenesis-abundant (LEA) protein genes in somatic embryos. The experiments show that the method can be used to obtain sufficient amounts of embryogenic material for basic molecular analyses.
Ikeda-Iwai
Miho
M
Gene Research Center, Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan. mikeda@sakura.cc.tsukuba.ac.jp
Satoh
Shinobu
S
Kamada
Hiroshi
H
D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000737:chemistry / Q000196:embryology* / Q000235:genetics; D029681:Arabidopsis Proteins; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D007150:Immunohistochemistry; D010940:Plant Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000378:metabolism; D012639:Seeds / Q000196:embryology* / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
C091601:ABI3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D022762:CCAAT-Enhancer-Binding Proteins; C113191:LEC1 protein, Arabidopsis; D010940:Plant Proteins; D018749:RNA, Plant; D014157:Transcription Factors; C066246:late embryogenesis abundant protein, plant; D000040:Abscisic Acid