Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes.
We used microarrays and real-time reverse transcription-PCR to analyze the global transcriptional response of Mycobacterium tuberculosis to low pH in vitro, which may mimic an environmental signal encountered by phagocytosed mycobacteria. Eighty-one genes were differentially expressed >1.5-fold, including many involved in fatty acid metabolism. The most highly induced genes showed homology with nonribosomal peptide synthetases/polyketide synthases.
Fisher
Mark A
MA
Program in Microbiology and Molecular Genetics, Emory University, Atlanta, Georgia 30322, USA.
Plikaytis
Bonnie B
BB
Shinnick
Thomas M
TM
D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism; D009097:Multienzyme Complexes / Q000096:biosynthesis; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010453:Peptide Synthases / Q000096:biosynthesis; D010588:Phagosomes / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D009097:Multienzyme Complexes; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase