Phosphatidyl ethanolamine is essential for targeting the arginine transporter Can1p to the plasma membrane of yeast.
In continuation of our previous study, we show that phosphatidyl ethanolamine (PE) depletion affects, in addition to amino acid transporters, activities of at least two other proton motive force (pmf)-driven transporters (Ura4p and Mal6p). For Can1p, we demonstrate that the lack of PE results in a failure of the permease targeting to plasma membrane. Despite the pleiotropic effect of PE depletion, a specific role of PE in secretion of a defined group of permeases can be distinguished. Pmf-driven transporters are more sensitive to the lack of PE than other plasma membrane proteins.
Opekarová
Miroslava
M
Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic. opekaro@biomed.cas.cz
Robl
Ingrid
I
Tanner
Widmar
W
D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism*; D026923:Amino Acid Transport Systems, Basic; D001120:Arginine / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D051246:Glucose Transport Proteins, Facilitative; D008364:Mannosyltransferases; D008565:Membrane Proteins / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D018892:Proton-Motive Force; D006180:Proton-Translocating ATPases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D033921:Vesicular Transport Proteins
D026905:Amino Acid Transport Systems; D026923:Amino Acid Transport Systems, Basic; C097640:CAN1 protein, Candida albicans; D005656:Fungal Proteins; D051246:Glucose Transport Proteins, Facilitative; C090594:HXT1 protein, S cerevisiae; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D010714:Phosphatidylethanolamines; C077883:SHR3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D033921:Vesicular Transport Proteins; C400810:ura4 protein, S pombe; C043909:arginine permease; D001120:Arginine; C090211:ANP1 protein, S cerevisiae; D008364:Mannosyltransferases; C092477:PMA1 protein, S cerevisiae; D006180:Proton-Translocating ATPases