[Protection of naoyi-an granule in ischemic brain injury secondary to intracerebral hemorrhage in rats].
OBJECTIVE
To explore the protective effect and its mechanism of the traditional Chinese medicine complex, naoyi-an granule(NYAG), on intracerebral hemorrhage(ICH) in rats.


METHODS
Collagenase-induced ICH rats were used. Inducible nitric oxide synthase(iNOS) activity of ischemic cerebral cortex surrounding the intracerebral hematoma was assayed by monitoring the conversion of L-3H-arginine to L-3H-citrulline. Pathologic changes in the same area were observed with transmission electron microscope on the 2nd day, the 4th day and the 7th day after ICH.


RESULTS
The iNOS activities of NYA-treated group decreased remarkably(P < 0.01) compared with those of the saline-treated group. Ultrastructural observation also indicated that NYAG ameliorated the brain edema, cell degeneration and necrosis.


CONCLUSIONS
The inhibitory effect of NYAG on iNOS activity may be related to its protection against ischemic brain injury secondary to ICH.
He
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G
Institute of Combined Traditional Chinese and Western Medicine, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Li
X
X
Liang
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Q
D000818:Animals; D002545:Brain Ischemia / Q000517:prevention & control*; D002543:Cerebral Hemorrhage / Q000188:drug therapy* / Q000201:enzymology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat