Application of rapid-cycle real-time polymerase chain reaction for the detection of microbial pathogens: the Mayo-Roche Rapid Anthrax Test.
Rapid-cycle real-time polymerase chain reaction has immediate and important implications for diagnostic testing in the clinical microbiology laboratory. In our experience this novel testing method has outstanding performance characteristics. The sensitivities for detecting microorganisms frequently exceed standard culture-based assays, and the time required to complete the assays is considerably shorter than that required for culture-based assays. We describe the principle of real-time polymerase chain reaction and present clinical applications, including the detection of Bacillus anthracis, the causative agent of anthrax. This latter test is commercially available as the result of a collaborative venture between Mayo Clinic and Roche Applied Science, hence the designation The Mayo-Roche Rapid Anthrax Test.
Uhl
James R
JR
Division of Clinical Microbiology, Mayo Clinic, Rochester, MN 55905, USA.
Bell
Constance A
CA
Sloan
Lynne M
LM
Espy
Mark J
MJ
Smith
Thomas F
TF
Rosenblatt
Jon E
JE
Cockerill
Franklin R
FR
D000881:Anthrax / Q000175:diagnosis; D001408:Bacillus anthracis / Q000235:genetics / Q000302:isolation & purification*; D001419:Bacteria / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial