title: Immunoglobulin fusion proteins as a tool for evaluation of T-cell costimulatory molecules.
abstract: Costimulatory signal(s) in addition to engagement of T cell receptor is important for optimal activation of T lymphocytes. During last decade several dozens of new costimulatory molecules, including 4-1BB, CD40, OX40, CD27, and ICOS, have been identified. It is likely that in Post-Genome Era more molecules with potential costimulatory properties will be brought to our attention. We describe here a simple and reliable strategy for evaluating the functional activities of potential costimulatory molecules using soluble fusion protein between extracellular portion of costimulatory ligand and Fc portion of mouse IgG2a.
authors.affiliation: Immunology Dept., Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Andrei I
authors.initials: AI
authors.lastname: Chapoval
authors.forename: Gefeng
authors.initials: G
authors.lastname: Zhu
authors.forename: Lieping
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D018122:B7-1 Antigen; D007141:Immunoglobulin Fc Fragments; D011993:Recombinant Fusion Proteins