Prognostic implication of apoptosis in human luteinized granulosa cells during IVF-ET.
OBJECTIVE
To investigate the prognostic significance of apoptosis in granulosa cells recovered from patients participating in IVF-ET.


METHODS
Seventy-three women underwent ovarian hyperstimulation for IVF and embryo transfer. After follicle aspiration, recovered granulosa cells were stained by BCL2 monoclonal antibody with FITC and propidium iodide (PI). Fluorescence was detected by flowcytometry, then the apoptotic index (Al) and BCL2 positivity were assessed.


RESULTS
The pregnant group showed a significantly lower apoptotic index in granulosa cells compared with the nonpregnant group (p < 0.0001). Patient's age, basal serum FSH concentration, serum E2 concentrations at hCG day, number of retrieved oocytes, fertilization rates. number of embryos transferred, and BCL2 positivity were not different between the two groups. Linear regression analysis of AI to serum basal FSH showed a positive correlation. Al in granulosa cells of 6.14% or below could predict a successful pregnancy with a sensitivity of 87.5% and a specificity of 73.7%.


CONCLUSIONS
Our results indicate that apoptotic analysis within granulosa cells can be used as a prognostic indicator for IVF success.
Suh
Chang Suk
CS
Department of Obstetrics and Gynecology, College of Medicine, Institute of Reproductive Medicine and Populations, Medical Research Center, Seoul National University, South Korea.
Jee
Byung Chul
BC
Choi
Young Min
YM
Kim
Jung Gu
JG
Lee
Jin Yong
JY
Moon
Shin Yong
SY
Kim
Seok Hyun
SH
D000328:Adult; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology*; D004624:Embryo Transfer; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D005434:Flow Cytometry; D005640:Follicle Stimulating Hormone / Q000097:blood; D006107:Granulosa Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D016014:Linear Models; D008297:Male; D011237:Predictive Value of Tests; D011247:Pregnancy; D011379:Prognosis; D011419:Propidium / Q000494:pharmacology; D012372:ROC Curve
D000911:Antibodies, Monoclonal; D011419:Propidium; D004958:Estradiol; D005640:Follicle Stimulating Hormone