Polyhydroxyalkanoate biosynthesis in Pseudomonas pseudoalcaligenes YS1.
Pseudomonas pseudoalcaligenes strain YS1 isolated from oil contaminated soil was able to produce polyhydroxybutyrate blended with medium-chain-length polyhydroxyalkanoates (mcl PHA). PHA synthesis genes were cloned from this strain. A fadB (gene for fatty acid degradation) deleted mutant Escherichia coli KM32B (FADB::Tet) was constructed to express the cloned PHA synthesis gene phaC1(Pp) or phaC2(Pp). The fadB deleted mutant KM32B harboring phaC1(Pp) or phaC2(Pp) showed mcl PHA accumulation while the intact E. coli KM32 did not. The results demonstrated that P. pseudoalcaligenes YS1 possessed at least two PHA synthesis pathways; one of them was responsible for production of mcl PHA.
Hang
Xiaoming
X
Department of Bioengineering, Dalian University of Technology, Dalian 116024, PR China.
Lin
Zhaoxing
Z
Chen
Jingyu
J
Wang
Guanlin
G
Hong
Kui
K
Chen
Guo-Qiang
GQ
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D017353:Gene Deletion; D011091:Polyesters / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D005227:Fatty Acids; D011091:Polyesters; D000217:Acyltransferases; C060970:poly-beta-hydroxybutyrate polymerase