Genetic heterogeneity of the pathogenic potentials of human and bovine group B streptococci.
One-hundred seventy-two B-streptococcal strains of human and bovine origin were analyzed for the presence of 9 genes potentially involved in virulence. Some of genes (glnA, cyl, hylB, scaA and cfb) were revealed in all the strains. However, the presence of others (bca, bac, scpB, lmb) varied from strain to strain. Taken together, 3 and 5 different types of pathogenic potential were found among human and bovine group B streptococci (GBS) strains, respectively, and only one type (bca+ bac scpB+ glnA+ cyl+ hylB+ lmb+ scaA+ cfb+) was common for both kinds of strains. We propose that different virulence genes can be involved in the development of infectious processes in humans and animals. A reliable PCR protocol with 3 pairs of primers (for the genes bca, bac and scpB) in the same reaction mixture was developed for the fast identification of the pathogenic potential of GBS. In comparison with the classical immunological methods this procedure displayed higher specificity and sensitivity as well as a shorter time of analysis. It can be recommended for use in the clinical and veterinary practice for studying the epidemiological relationship between the isolates and the ready identification of the clone causing the infection.
Dmitriev
A
A
Institute of Experimental Medicine, St. Petersburg 197 376, Russia.
Shakleina
E
E
Tkáciková
L
L
Mikula
I
I
Totolian
A
A
D000818:Animals; D015373:Bacterial Typing Techniques / Q000191:economics / Q000379:methods; D002417:Cattle; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial; D014644:Genetic Variation; D006801:Humans; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000453:epidemiology / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics*