title: Genome expression analysis of Anopheles gambiae: responses to injury, bacterial challenge, and malaria infection.
abstract: The complex gene expression responses of Anopheles gambiae to microbial and malaria challenges, injury, and oxidative stress (in the mosquito and/or a cultured cell line) were surveyed by using cDNA microarrays constructed from an EST-clone collection. The expression profiles were broadly subdivided into induced and down-regulated gene clusters. Gram+ and Gram- bacteria and microbial elicitors up-regulated a diverse set of genes, many belonging to the immunity class, and the response to malaria partially overlapped with this response. Oxidative stress activated a distinctive set of genes, mainly implicated in oxidoreductive processes. Injury up- and down-regulated gene clusters also were distinctive, prominently implicating glycolysis-related genes and citric acid cycle/oxidative phosphorylation/redox-mitochondrial functions, respectively. Cross-comparison of in vivo and in vitro responses indicated the existence of tightly coregulated gene groups that may correspond to gene pathways.
authors.affiliation: Department of Biological Sciences, Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, London SW7 2AZ, United Kingdom.
authors.forename: George
authors.initials: G
authors.lastname: Dimopoulos
authors.forename: George K
authors.initials: GK
authors.lastname: Christophides
authors.forename: Stephan
authors.initials: S
authors.lastname: Meister
authors.forename: Jörg
authors.initials: J
authors.lastname: Schultz
authors.forename: Kevin P
authors.initials: KP
authors.lastname: White
authors.forename: Carolina
authors.initials: C
authors.lastname: Barillas-Mury
authors.forename: Fotis C
authors.initials: FC
authors.lastname: Kafatos
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000469:parasitology; D018076:DNA, Complementary; D005260:Female; D016678:Genome; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D010961:Plasmodium / Q000472:pathogenicity*
chemical_list: D018076:DNA, Complementary