[The effects of IGF-II on the proliferation and differentiation of human osteoblast-like cells].
Biological regulatory mechanism which supports bone cell development within the context of proliferation/differentiation relationship serves as a viable basis for addressing skeletal disorders and provides experiment and theoretic basis for selection and utilization of growth factors to reconstruct bone defect. Osteoblast-like cells isolated from human calvarium were used in this study. IGF-II were investigated by adding in the culture medium with different concentration. The results showed that IGF-II stimulated DNA, collagen synthesis and ALP activity of human osteoblast-like cells. Its effects were strong for proliferation and differentiation of osteoblast cells, when 10 ng/ml of four different concentrations (0.1, 1, 10 and 100 ng/ml) were used. IGF-II was proved to be important factor in bone regeneration.
Tian
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Department of Oral and Maxillofacial Surgery, West China University of Medical Sciences.
Wang
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D000469:Alkaline Phosphatase / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D005333:Fetus; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D012886:Skull / Q000166:cytology
D007335:Insulin-Like Growth Factor II; D003094:Collagen; D004247:DNA; D000469:Alkaline Phosphatase