title: Tryptophan decarboxylase from transformed roots of Catharanthus roseus.
abstract: Tryptophan decarboxylase (TDC, EC 4.1.1.28) from Catharanthus roseus hairy roots was purified 80-fold. Antibodies against TDC were obtained and they recognized only one protein of 55 kDa in crude extracts from hairy root cultures. Elicitation of transformed root cultures with macerozyme yielded a marked increase in TDC activity, which was accompanied by a similar increase in the amount of immunoreactive TDC protein. These results suggest that the alkaloid accumulation, produced by elicitation, requires the synthesis of new TDC polypeptide in C. roseus root cultures and establishes important differences in the regulatory control of this enzyme in root cultures compared to developing seedlings, where the posttranslational regulation apparently plays a major role.
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigacíon Científica de Yucatán, México.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Islas-Flores
authors.forename: Oscar
authors.initials: O
authors.lastname: Moreno-Valenzuela
authors.forename: Yereni
authors.initials: Y
authors.lastname: Minero-García
authors.forename: Victor M
authors.initials: VM
authors.lastname: Loyola-Vargas
authors.forename: María de Lourdes
authors.initials: Mde L
authors.lastname: Miranda-Ham
mesh_terms: D000470:Alkaloids / Q000378:metabolism; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D030008:Catharanthus / Q000201:enzymology* / Q000235:genetics; D046508:Culture Techniques; D015151:Immunoblotting; D018517:Plant Roots / Q000201:enzymology* / Q000235:genetics; D030821:Plants, Genetically Modified
chemical_list: D000470:Alkaloids; D001142:Aromatic-L-Amino-Acid Decarboxylases