Cytogenetic analysis of an asymmetric potato hybrid.
An asymmetric potato hybrid and its parental lines were cytogenetically examined. DAPI (4'-6-diamidino-2-phenylindole) staining was used to count chromosomes in all analysed lines and revealed the presence of minichromosomes in the hybrid genome. Fluorescent in situ hybridization (FISH) with rDNA sequence as a probe helped to determine the ploidy level of analysed lines and revealed that none of the minichromosomes contains rDNA repeats. CMA (chromomycin A3) band occurred to be a new chromosome marker that identifies potato chromosome No.1. It was possible to detect a deletion in one of four chromosomes No. 1 of the asymmetric potato hybrid. On the basis of these analyses a karyotype of the asymmetric hybrid was constructed.
Srebniak
Małgorzata
M
Department of Plant Anatomy and Cytology, Silesian University, ul. Jagiellonska 28, 40-032 Katowice, Poland.
Rasmussen
Ole
O
Małuszyńska
Jolanta
J
D004275:DNA, Ribosomal / Q000235:genetics; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D012091:Repetitive Sequences, Nucleic Acid; D011198:Solanum tuberosum / Q000235:genetics*
D004275:DNA, Ribosomal