Identification and punctate nuclear localization of a novel noncoding RNA, Ks-1, from the honeybee brain.
We identified a novel gene, Ks-1, which is expressed preferentially in the small-type Kenyon cells of the honeybee brain. This gene is also expressed in some of the large soma neurons in the brain and in the suboesophageal ganglion. Reverse transcription-polymerase chain reaction experiments indicated that Ks-1 transcripts are enriched in the honeybee brain. cDNA cloning revealed that the consensus Ks-1 cDNA is over 17 kbp and contains no significant open reading frames. Furthermore, fluorescent in situ hybridization revealed that Ks-1 transcripts are located in the nuclei of the neural cells, accumulating in some scattered spots. These findings demonstrate that Ks-1 encodes a novel class of noncoding nuclear RNA and is possibly involved in the regulation of neural functions.
Sawata
Miyuki
M
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Japan.
Yoshino
Daisuke
D
Takeuchi
Hideaki
H
Kamikouchi
Azusa
A
Ohashi
Kazuaki
K
Kubo
Takeo
T
D000818:Animals; D001483:Base Sequence; D001516:Bees; D001921:Brain / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D018076:DNA, Complementary; D005260:Female; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000378:metabolism; D022661:RNA, Untranslated / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D018076:DNA, Complementary; D012333:RNA, Messenger; D022661:RNA, Untranslated