MaGiK method of T-Cell receptor repertoire analysis.
T-cell receptor diversity enables the cellular immune response to recognize a broad range of viral and other pathogenic agents. An increasingly common method of characterizing T-cell receptor diversity and usage in response to antigenic challenges involves the identification of clonal expansions by PCR amplification of the CDR3 region of distinct TCRVbeta families. Though clonal expansions often appear evident upon visual inspection of the results, a systematic method is needed for the valid enumeration of these expansions. Here, we describe a novel analysis method, termed the MaGiK method, for systematically identifying and enumerating clonal T-cell expansions and for applying the results to investigations of the T-cell receptor repertoire.
Killian
M Scott
MS
Department of Epidemiology, University of California, Los Angeles, California 90095, USA. skill@ucla.edu
Matud
Jose
J
Detels
Roger
R
Giorgi
Janis V
JV
Jamieson
Beth D
BD
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D022801:Complementarity Determining Regions / Q000235:genetics; D005260:Female; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000235:genetics*; D013223:Statistics as Topic; D016176:T-Lymphocyte Subsets / Q000276:immunology
D022801:Complementarity Determining Regions; D011948:Receptors, Antigen, T-Cell