In vitro and in vivo characteristics of a human colon cancer cell line, SNU-C5N, expressing sodium-iodide symporter.
Rat NIS (rNIS) genes were transfected into a human colon cancer cell line (SNU-C5) by lipofection. The transfected cells (SNU-C5N) exhibited an increase 125I uptake to a level 10 times higher than the untransfected SNU-C5 cells. The addition of 300 microM DIDS, an anion channel blocker, to the culture media led to a 2.35 times increase of 125I uptake in the cells. For the first 10 minutes, up to 70% of the cellular radioactivity was released into the medium. In the biodistribution study using SNU-C5N-xenografted mice, the %ID/g of the SNU-C5N tumors at 1, 3, 6, and 12 h after the 125I injection were 4.43%, 1.09%, 1.05%, and 0.05%, respectively, which were significantly higher than those for the SNU-C5 tumors (P<0.05). In tumor imaging, the SNU-C5N-xenografted tumor was clearly visible. In this study, NIS lipofection is efficient for triggering significant iodide uptake by a nonthyroidal tumor. However, for an increased therapeutic effect, the key issue is iodide retention in the target tissue.
Min
Jung-Jun
JJ
Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea.
Chung
June-Key
JK
Lee
Yong Jin
YJ
Shin
Jae Hoon
JH
Yeo
Jeong Seok
JS
Jeong
Jae Min
JM
Lee
Dong Soo
DS
Bom
Hee-Seung
HS
Lee
Myung Chul
MC
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000819:agonists / Q000494:pharmacology; D000818:Animals; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics*; D008081:Liposomes; D008094:Lithium / Q000494:pharmacology; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D010472:Perchlorates / Q000494:pharmacology; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D012680:Sensitivity and Specificity; D017670:Sodium Compounds / Q000494:pharmacology; D027981:Symporters / Q000378:metabolism*; D013848:Thigh; D014018:Tissue Distribution; D014162:Transfection; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D014915:Whole-Body Counting; D000099209:Sodium-Iodide Symporters
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D007457:Iodine Radioisotopes; D008081:Liposomes; D008094:Lithium; D010472:Perchlorates; D019275:Radiopharmaceuticals; D017670:Sodium Compounds; D027981:Symporters; D000099209:Sodium-Iodide Symporters; C031068:sodium perchlorate