title: Lactoferrin iron levels are reduced in saliva of patients with localized aggressive periodontitis.
abstract: BACKGROUND
  Actinobacillus actinomycetemcomitans (Aa) is associated with localized aggressive periodontal disease in juveniles (LAgP). Lactoferrin (LF) is an iron-binding salivary protein that has been shown to kill Aa in its iron-free form (apo) and reduce binding to host cells in its iron-saturated form (halo). However, recent in vitro studies show that LF does not kill clinical isolates of Aa, and LF with reduced levels of bound iron does not interfere with its attachment. These findings suggest that colonization of Aa may occur more readily in an environment containing LF with low iron levels. The purpose of this study was to examine the relationship of LF iron levels in saliva of LAgP patients as compared to their age-, gender-, and race-matched controls.
  
  
  METHODS
  Whole and parotid saliva was collected from LAgP patients and matched controls. Micrograms of LF/mg of protein as well as nanograms of iron/micrograms of LF were determined. Iron binding was determined in parotid saliva by addition of nonlabeled and 59Fe labeled iron.
  
  
  RESULTS
  LAgP patients' whole saliva had higher LF levels than controls, but their LF contained less iron (P < or =0.005). No iron was found in LF from parotid saliva in either group. When iron was added to parotid saliva, the LAgP saliva bound 20 to 30 times less iron than controls (P< or =0.001). Finally, LF was identified as the major iron-binding protein in parotid saliva by 59Fe autoradiography and Western blotting.
  
  
  CONCLUSIONS
  This study shows that the level of bound iron in LF is significantly reduced in LAgP patients compared to controls. These data suggest that LF from LAgP patients has a reduced capacity to bind iron and that LF iron levels may play an important role in Aa-induced LAgP.
authors.affiliation: Department of Oral Pathology and Biology, University of Medicine and Dentistry of New Jersey, New Jersey Dental School, Newark 07103, USA. finedh@umdnj.edu
authors.forename: D H
authors.initials: DH
authors.lastname: Fine
authors.forename: D
authors.initials: D
authors.lastname: Furgang
authors.forename: F
authors.initials: F
authors.lastname: Beydouin
mesh_terms: D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000378:metabolism*; D001345:Autoradiography; D015153:Blotting, Western; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007501:Iron / Q000032:analysis / Q000378:metabolism*; D007504:Iron Radioisotopes / Q000378:metabolism; D007781:Lactoferrin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D012463:Saliva / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000378:metabolism*; D018709:Statistics, Nonparametric
chemical_list: D007504:Iron Radioisotopes; D012471:Salivary Proteins and Peptides; D007501:Iron; D007781:Lactoferrin