title: Chitinases A, B, and C1 of Serratia marcescens 2170 produced by recombinant Escherichia coli: enzymatic properties and synergism on chitin degradation.
abstract: To discover the individual roles of the chitinases from Serratia marcescens 2170, chitinases A, B, and C1 (ChiA, ChiB, and ChiC1) were produced by Escherichia coli and their enzymatic properties as well as synergistic effect on chitin degradation were studied. All three chitinases showed a broad pH optimum and maintained significant chitinolytic activity between pH 4 and 10. ChiA was the most active enzyme toward insoluble chitins, but ChiC1 was the most active toward soluble chitin derivatives among the three chitinases. Although all three chitinases released (GlcNAc)2 almost exclusively from colloidal chitin, ChiB and ChiC1 split (GlcNAc)6 to (GlcNAc)3, while ChiA exclusively generated (GlcNAc)2 and (GlcNAc)4. Clear synergism on the hydrolysis of powdered chitin was observed in the combination between ChiA and either ChiB or ChiC, and the sites attacked by ChiA on the substrate are suggested to be different from those by either ChiB or ChiC1.
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Japan.
authors.forename: Kazushi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Noriko
authors.initials: N
authors.lastname: Sugawara
authors.forename: Megumi
authors.initials: M
authors.lastname: Suzuki
authors.forename: Taku
authors.initials: T
authors.lastname: Uchiyama
authors.forename: Fuminori
authors.initials: F
authors.lastname: Katouno
authors.forename: Naoki
authors.initials: N
authors.lastname: Nikaidou
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006868:Hydrolysis; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011995:Recombination, Genetic; D012706:Serratia marcescens / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D009844:Oligosaccharides; D011994:Recombinant Proteins; D002686:Chitin; D002688:Chitinases