Time course of humoral and cell-mediated immune responses to human papillomavirus type 16 in infected women.
The time course of cell-mediated and humoral immune responses was elucidated in eight women with human papillomavirus type 16 (HPV-16) infection by performing serial HPV-16 E6 and E7 cytotoxic T-lymphocyte (CTL) assays and HPV-16 virus-like particle (VLP) antibody analyses. Four subjects had a single incident of HPV-16 DNA detection, and four subjects had two periods of HPV-16 DNA detection. In two of the women in the latter group, the second episode of HPV-16 detection occurred in the presence of high titers of HPV-16 VLP antibody, bringing into question the protective role of humoral immunity in preventing repeated infection. However, all four subjects rapidly became HPV-16 DNA negative following the second detection of HPV-16 DNA, suggesting the presence of immunological memory. In addition, one subject rapidly became negative for HPV-16 DNA despite having no evidence of CTL or VLP antibody response prior to the second HPV-16 DNA detection, suggesting the presence of immunological responses at an undetectable level. Overall, seven of eight subjects (88%) had detectable HPV-16 E6 and/or E7 CTL responses and seven of eight women (88%) had detectable HPV-16 VLP antibody responses.
Nakagawa
Mayumi
M
Departments of Laboratory Medicine, Schools of Medicine and Dentistry, University of California at San Francisco, San Francisco, California 94143-0134, USA. nakagawa@labmed2.ucsf.edu
Viscidi
Raphael
R
Deshmukh
Ian
I
Costa
Maria Da
MD
Palefsky
Joel M
JM
Farhat
Sepideh
S
Moscicki
Anna-Barbara
AB
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D036022:Capsid Proteins; D004279:DNA, Viral / Q000097:blood; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008137:Longitudinal Studies; D009856:Oncogene Proteins, Viral / Q000276:immunology; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology*; D050725:Papillomavirus E7 Proteins; D030361:Papillomavirus Infections / Q000276:immunology*; D012008:Recurrence; D012097:Repressor Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014412:Tumor Virus Infections / Q000276:immunology
D000914:Antibodies, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D007074:Immunoglobulin G; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; C063317:L1 protein, Human papillomavirus type 16