title: MUTZ-3, a human cell line model for the cytokine-induced differentiation of dendritic cells from CD34+ precursors.
abstract: Many human myeloid leukemia-derived cell lines possess the ability to acquire a dendritic cell (DC) phenotype. However, cytokine responsiveness is generally poor, requiring direct manipulation of intracellular signaling mechanisms for differentiation. In contrast, the CD34+ human acute myeloid leukemia cell line MUTZ-3 responds to granulocyte macrophage- colony-stimulating factor (GM-CSF), interleukin 4 (IL-4), and tumor necrosis factor alpha (TNFalpha), cytokines known to be pivotal both in vivo and in vitro for DC generation from monocytes and CD34+ stem cells. In all respects, MUTZ-3 cells behave as the immortalized equivalent of CD34+ DC precursors. Upon stimulation with specific cytokine cocktails, they acquire a phenotype consistent with either interstitial- or Langerhans-like DCs and upon maturation (mDC), express CD83. MUTZ-3 DC display the full range of functional antigen processing and presentation pathways. These findings demonstrate the unique suitability of MUTZ-3 cells as an unlimited source of CD34+ DC progenitors for the study of cytokine-induced DC differentiation.
authors.affiliation: Department of Medical Oncology, Division Immunotherapy, VU University Medical Center VUmc, Amsterdam, The Netherlands.
authors.forename: Allan J
authors.initials: AJ
authors.lastname: Masterson
authors.forename: Claudia C
authors.initials: CC
authors.lastname: Sombroek
authors.forename: Tanja D
authors.initials: TD
authors.lastname: De Gruijl
authors.forename: Yvo M F
authors.initials: YM
authors.lastname: Graus
authors.forename: Hans J J
authors.initials: HJ
authors.lastname: van der Vliet
authors.forename: Sinéad M
authors.initials: SM
authors.lastname: Lougheed
authors.forename: Alfons J M
authors.initials: AJ
authors.lastname: van den Eertwegh
authors.forename: Herbert M
authors.initials: HM
authors.lastname: Pinedo
authors.forename: Rik J
authors.initials: RJ
authors.lastname: Scheper
mesh_terms: D000938:Antigen-Presenting Cells / Q000166:cytology; D015703:Antigens, CD; D018952:Antigens, CD34 / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D016130:Immunophenotyping; D008562:Membrane Glycoproteins / Q000032:analysis; D008954:Models, Biological; D014407:Tumor Cells, Cultured / Q000166:cytology*; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D016207:Cytokines; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen