title: Cloning, Expression and Tumor Suppression of Human Endostatin.
abstract: Human endostatin cDNA was cloned from total RNA of normal Chinese liver cell line L02 by RT-PCR. Endostatin DNA sequence encoded 184 amino acid residues. Five base pairs and 3 amino acid residues are different from that reported, it may be due to interspecies difference. The endostatin cDNA was inserted into the pET-28a(+) containing T7 promoter. The recombinant plasmid was transformed the E.coli BL21(DE3). Recombinant human endostatin was highly expressed as inclusion body when the expression strain BL-ENDO was induced with 1 mmol/L IPTG. Result of SDS-PAGE analysis revealed that recombinant human endostatin was accounted for up to 25% of soluble protein in E.coli. Purified and refolded recombinant human endostatin was active in inhibiting tumor growth and metastasis.
authors.affiliation: Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. xfwu@sunm.shcnc.ac.cn
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: He
authors.forename: Zhe-Yu
authors.initials: ZY
authors.lastname: Chen
authors.forename: Cun-Ping
authors.initials: CP
authors.lastname: Qiu
authors.forename: Biao
authors.initials: B
authors.lastname: Li
authors.forename: Wei-Jie
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Xiang-Fu
authors.initials: XF
authors.lastname: Wu