Dynamics of CD4 and CD8 T cell responses to cytomegalovirus in healthy human donors.
To study the dynamics of cytomegalovirus (CMV) immunity in healthy immunocompetent hosts, interferon-gamma-producing CD4 and CD8 T cell responses in the presence or absence of CMV antigens were measured from 15 CMV-seropositive donors and 13 CMV-seronegative donors. Cytokine responses in the absence of antigen were significantly higher in CMV-seropositive donors. Also, a disproportionate number of CD69(+) cells isolated ex vivo from CMV-seropositive donors were specific for CMV, suggesting recent reactivation in vivo. To examine changes in cellular responses over time, 10 seropositive donors were tested over a 6-month period. About half of the donors showed significant variability over time, but staphylococcal enterotoxin B responses remained relatively constant. These findings suggest that CMV can present a considerable and recurrent burden to the human immune system. By understanding the normal dynamics of CMV responses over time, it may be possible to better identify aberrant responses to CMV in immunocompromised hosts.
Dunn
Holli S
HS
BD Biosciences, Immunocytometry Systems, San Jose, CA 95131, USA. holli_dunn@bdis.com
Haney
Douglas J
DJ
Ghanekar
Smita A
SA
Stepick-Biek
Pamela
P
Lewis
David B
DB
Maecker
Holden T
HT
D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis; D000956:Antigens, Viral; D001782:Blood Donors; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis; D003587:Cytomegalovirus / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis
D000942:Antigens, Bacterial; D015703:Antigens, CD; D000956:Antigens, Viral; D016207:Cytokines; D004768:Enterotoxins; D014409:Tumor Necrosis Factor-alpha; C031818:enterotoxin B, staphylococcal; D007371:Interferon-gamma