Tritrichomonas foetus: induced division synchrony by hydroxyurea.
Treatment of cultures of Tritrichomonas foetus with 4 mM hydroxyurea (HU), a known DNA synthesis inhibitor, induced pseudocyst formation and caused a mitotic burst. An hour after drug release there was a characteristic, synchronous burst of cell division. T. foetus culture was arrested in the G2/M phase. The synchrony index varied from 66% to 69%. The synchrony was maintained for several cell cycles, even in thawed cultures which had been frozen for storage in liquid nitrogen. The synchronized cells were analyzed by light and scanning electron microscopy, as well by flow cytometry.
Ribeiro
Karla Consort
KC
Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, Campos, RJ, Brazil.
Arnholdt
Andrea C Vetö
AC
Benchimol
Marlene
M
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D016195:G2 Phase / Q000187:drug effects; D006918:Hydroxyurea / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D008938:Mitosis / Q000187:drug effects; D014244:Trichomonas / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure
D006918:Hydroxyurea