[Determination of three flavonoids and ferulic acid in Pleurospermum hookeri C. B. Clarke var. thomsonii C. B. Clarke by HPLC].
OBJECTIVE
To establish a reversed phase HPLC method for separation and determination of licochalcone A, ferulic acid, isoliquiritigenin and nobiletin in Pleurospermum hookeri var. thomsonii.


METHODS
Using ODS column with methanol-water(containing 1% acetic acid)(73:27, 28:72, 59:41) and methanol-water(65:35) as the mobile phase, the flow rate was 1 ml/min, the column temperature was 35 degrees C and UV detection at 345, 320, 345, 332 nm, different extraction methods for the sample were compared.


RESULTS
The average recoveries were 99.45%, 98.13%, 97.81% and 96.58%, the relative standard deviations were 0.83%, 0.99%, 0.73% and 1.55%, and the contents were 0.728, 0.124, 0.0830 and 0.0237 mg/g.


CONCLUSIONS
This study provides a scientific basis for quality evaluation, quality control and resources development and utilization of P. hookeri var. thomsonii.
Li
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T
School of Pharmacy, West China University of Medical Sciences, Chengdu 610041.
Wang
T
T
D019661:Apiaceae / Q000737:chemistry*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000737:chemistry*; D047188:Chalcones; D003373:Coumaric Acids / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000592:standards; D047309:Flavones; D005419:Flavonoids / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
D047188:Chalcones; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D047309:Flavones; D005419:Flavonoids; D002599:Chalcone; C004999:ferulic acid; C040920:isoliquiritigenin; C008661:nobiletin; C070840:licochalcone A