Neuritogenesis of herbal (+)- and (-)-syringaresinols separated by chiral HPLC in PC12h and Neuro2a cells.
Syringaresinol isolated from Epimedium koreanum NAKA1 and Magnolia officinalis REHD. et WILS. was subjected to optical resolution by chiral HPLC to give (+)- and (-)-enantiomers. The two syringaresinol enantiomers, as well as a mixture of their glucosides, showed dose-dependent neuritogenesis in a concentration range from 0.24 to 24 microM in PC12h cells.
Chiba
Kenzo
K
Department of Biodynamics, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Ishikawa, Japan. k-chiba@hokuriku-u.ac.jp
Yamazaki
Matsumi
M
Umegaki
Emiko
E
Li
Ming Run
MR
Xu
Zhen Wen
ZW
Terada
Sumio
S
Taka
Michihiro
M
Naoi
Noriyuki
N
Mohri
Tetsuro
T
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D034181:Epimedium / Q000737:chemistry*; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D031566:Magnolia / Q000737:chemistry*; D009416:Nerve Regeneration / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects; D016716:PC12 Cells; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D005663:Furans; D017705:Lignans; C042192:syringaresinol