title: Pathogenicity and protective activity in pregnant goats of a Brucella melitensis Deltaomp25 deletion mutant.
abstract: The Brucella melitensis mutant BM 25, which lacks the major 25 kDa outer membrane protein Omp25, has previously been found to be attenuated in the murine brucellosis model. In the present study, the capacity of the Deltaomp25 mutant to colonise and cause abortions in the caprine host was evaluated. The vaccine potential of BM 25 was also investigated in goats. Inoculation of nine pregnant goats in late gestation with the B. melitensis mutant resulted in 0/9 abortions, while the virulent parental strain, B. melitensis 16M, induced 6/6 dams to abort (P<0.001, n=6). BM 25 also colonised fewer adults (P<0.05, n=6) and kids (P<0.01, n=6) than strain 16M. The Deltaomp25 mutant was found capable of transient in vivo colonisation of non-pregnant goats for two weeks post-infection. Owing to the ability of BM 25 to colonise both non-pregnant and pregnant adults without inducing abortions, a vaccine efficacy study was performed. Vaccination of goats prior to breeding with either BM 25 or the current caprine vaccine B. melitensis strain Rev. 1 resulted in 100 per cent protection against abortion following challenge in late gestation with virulent strain 16M (P<0.05, n=7). However, unlike strain Rev. 1, BM 25 does not appear to cause abortions in late gestation based on this study with a small number of animals. The B. melitensis Deltaomp25 mutant, BM 25, may be a safe and efficacious alternative to strain Rev. 1 when dealing with goat herds of mixed age and pregnancy status.
authors.affiliation: Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Edmonds
authors.forename: A
authors.initials: A
authors.lastname: Cloeckaert
authors.forename: S D
authors.initials: SD
authors.lastname: Hagius
authors.forename: L E
authors.initials: LE
authors.lastname: Samartino
authors.forename: W T
authors.initials: WT
authors.lastname: Fulton
authors.forename: J V
authors.initials: JV
authors.lastname: Walker
authors.forename: F M
authors.initials: FM
authors.lastname: Enright
authors.forename: N J
authors.initials: NJ
authors.lastname: Booth
authors.forename: P H
authors.initials: PH
authors.lastname: Elzer
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines; D017347:Brucella melitensis / Q000235:genetics* / Q000472:pathogenicity; D002006:Brucellosis / Q000662:veterinary*; D002352:Carrier Proteins / Q000235:genetics* / Q000276:immunology; D005260:Female; D017353:Gene Deletion; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology; D011247:Pregnancy
chemical_list: D001428:Bacterial Vaccines; D002352:Carrier Proteins; D008565:Membrane Proteins; C120338:Synj2bp protein, rat