Crystallographic study of a naturally occurring trans-splicing intein from Synechocystis sp. PCC 6803.
A naturally occurring split intein from the dnaE gene of Synechocystis sp. PCC6803 (Ssp DnaE intein) has been purified and crystallized using PEG 8K as precipitant. The crystal belongs to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 58.5, c = 70.2 A. It has one molecule per asymmetric unit and diffracts to beyond 2.9 A under cryoconditions (110 K) using a Cu rotating-anode X-ray generator in-house.
Chen
Xuehui
X
Laboratory of Structural Biology, Department of Biological Science and Technology and MOE Laboratory of Protein Science, Tsinghua University, Beijing 100084, People's Republic of China.
Xu
Ming Qun
MQ
Ding
Yi
Y
Ferrandon
Sébastien
S
Rao
Zihe
Z
D017398:Alternative Splicing; D018360:Crystallography, X-Ray / Q000379:methods*; D000458:Cyanobacteria / Q000737:chemistry* / Q000378:metabolism; D004258:DNA Polymerase III / Q000737:chemistry* / Q000302:isolation & purification; D010957:Plasmids / Q000378:metabolism; D013696:Temperature
C074904:DNA polymerase III, alpha subunit; D004258:DNA Polymerase III