title: Expression, purification, crystallization and preliminary X-ray analysis of rat ecto-ADP-ribosyltransferase 2 (ART2.2).
abstract: ADP-ribosyltransferases catalyze the transfer of the ADP-ribose moiety from NAD(+) onto proteins and other targets. These enzymes have been found in prokaryotes and in vertebrates; a eukaryotic enzyme structure is not yet known. The enzyme from Rattus norvegicus was expressed in the Escherichia coli periplasm at a level of about 0.2 mg per litre of culture, purified and crystallized. Native data sets were collected to 2.0 A resolution. A self-rotation function revealed a local twofold axis in crystal form A and a Patterson function showed a translational relationship in form B. Form C contains only one molecule in the asymmetric unit.
authors.affiliation: Institut für Organische Chemie und Biochemie, Albertstrasse 21, Freiburg im Breisgau 79104, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Mueller-Dieckmann
authors.forename: Till
authors.initials: T
authors.lastname: Scheuermann
authors.forename: Karsten
authors.initials: K
authors.lastname: Wursthorn
authors.forename: Jens
authors.initials: J
authors.lastname: Schröder
authors.forename: Friedrich
authors.initials: F
authors.lastname: Haag
authors.forename: Georg E
authors.initials: GE
authors.lastname: Schulz
authors.forename: Friedrich
authors.initials: F
authors.lastname: Koch-Nolte
mesh_terms: D036002:ADP Ribose Transferases / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D002460:Cell Line; D006224:Cricetinae; D018360:Crystallography, X-Ray / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007313:Insecta; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D019897:Periplasm / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D051381:Rats; D013696:Temperature; D013997:Time Factors
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D008562:Membrane Glycoproteins; D036002:ADP Ribose Transferases; C090163:Art2b protein, rat