title: In vitro assessment of antiborrelial activity of OspA vaccine sera.
abstract: Prevention of Lyme disease by the recombinant OspA-based vaccine reportedly works by preventing transmission of spirochetes from ticks to humans. We report on an in vitro microculture assay, which can be used to provide an indicator of the need for booster doses of vaccine.
authors.affiliation: Immunology Laboratory, Department of Research, A. I. duPont Hospital for Children, Wilmington, Delaware 19803, USA. pfawcett@nemours.org
authors.forename: Paul T
authors.initials: PT
authors.lastname: Fawcett
authors.forename: Carlos D
authors.initials: CD
authors.lastname: Rose
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Gibney
mesh_terms: D000954:Antigens, Surface / Q000008:administration & dosage*; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage*; D001428:Bacterial Vaccines; D025065:Borrelia burgdorferi / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D008074:Lipoproteins; D011237:Predictive Value of Tests; D012698:Serologic Tests / Q000592:standards; D014611:Vaccination / Q000592:standards; D014775:Virus Activation / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D007106:Immune Sera; D008074:Lipoproteins; C072045:OspA protein