Cicerin and arietin, novel chickpea peptides with different antifungal potencies.
Two antifungal peptides with novel N-terminal sequences, designated cicerin and arietin were isolated from seeds of the chickpea (Cicer arietinum), respectively. Both peptides were adsorbed on Affi-gel blue gel and CM-Sepharose and exhibited a molecular weight of approximately 8.2 and 5.6 kDa, respectively. Arietin was more strongly adsorbed on CM-Sepharose than cicerin and manifested a higher translation-inhibiting activity in a rabbit reticulocyte lysate system and a higher antifungal potency toward Mycosphaerella arachidicola, Fusarium oxysporum and Botrytis cinerea. Both were devoid of mitogenic and anti-HIV-1 reverse transcriptase activities.
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Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Basic Medical Sciences Building, Shatin, NT, Hong Kong, China.
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Rao
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D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002846:Chromatography, Affinity; D029863:Cicer / Q000737:chemistry*; D005658:Fungi / Q000166:cytology / Q000187:drug effects; D020128:Inhibitory Concentration 50; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry
D000935:Antifungal Agents; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D010940:Plant Proteins; C475601:cicerin protein, Cicer arietinum; C069270:arietin