[Study on the adhesion of an endemic strain of Streptococcus mutans serotype C to acquired pellicle. I. A comparison of the methods to extract adhesins from Streptococcus mutans].
The adhesins of S. mutans serotype c endemic strain WD9463A were extracted from culture superant by cold ethol precipitation, or were extracted from cell surface by 0.5 mol/L phosphate buffer, 6 mol/L urea or 2% SDS solution. The composition and adhesive activities of the extracts were compared by SDS-PAGE and bacterial adhesion inhibition experiments. The results showed that the protein composition of the four extracts were similar, and all extracts inhibited the adhesion of the endemic strain dramatically. It indicates that four methods can be used to extract adhesins of S. mutans, but extracting adhesins from culture superant is better than the others.
Zhan
L
L
College of Stomatology, West China University of Medical Sciences.
Liu
T
T
Yue
S
S
D018829:Adhesins, Bacterial / Q000302:isolation & purification*; D001422:Bacterial Adhesion; D044622:Dental Pellicle; D013295:Streptococcus mutans / Q000737:chemistry* / Q000502:physiology
D018829:Adhesins, Bacterial