Positional cloning of an obesity/diabetes susceptibility gene(s) on chromosome 11 in Pima Indians.
Prior results from our genomic scan in Pima Indians indicated an obesity locus in a region on chromosome 11q23-24 that was also linked to diabetes. Bivariate linkage analysis for the combined phenotype "diabesity" gave the strongest evidence for linkage (LOD = 5.2). Our aim is to positionally clone the gene(s) responsible for the linkage. Linkage disequilibrium mapping is being used to narrow the chromosomal region. Single nucleotide polymorphisms (SNPs) are being systematically identified and genotyped at 50-kb intervals across the region of linkage. To date, 455 SNPs have been genotyped in 1229 Pimas. A region containing a cluster of SNPs strongly associated with BMI and a second region, approximately 2 Mb telomeric, containing a cluster of SNPs associated with diabetes have been preliminarily identified.
Baier
Leslie
L
Phoenix Epidemiology and Clinical Research Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Arizona 85016, USA. lbaier@phx.niddk.nih.gov
Kovacs
Peter
P
Wiedrich
Christopher
C
Cray
Kimberly
K
Schemidt
Amy
A
Shen
Gong-Qing
GQ
Sutherland
Jeffrey
J
Thuillez
Pamela
P
Muller
Yunhua Li
YL
Traurig
Michael
M
Bogardus
Clifton
C
D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D006801:Humans; D007198:Indians, North American / Q000235:genetics*; D015810:Linkage Disequilibrium; D009765:Obesity / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide