Detection of genetically modified crops and their derivatives: critical steps in sample preparation and extraction.
The detection of genetically modified crops in foodstuff relies on detection of transgenic DNA or protein material in the sample matrix. Purified DNA or proteins are used as analytical material for polymerase chain reaction technologies and immunodiagnostics. Successful sample preparation is critical to the validity of subsequent analysis. For routine analysis, a good sample preparation technique should be simple, safe, and inexpensive while reproducibly generating DNA/protein of sufficient quality and yield. The suitability of isolated DNA or protein as an analyte for a detection or characterization technique depends on amount or concentration, purity, and integrity, each of which may be influenced by sample matrix and the extraction technique, and, in turn, may impact the validity of analytical techniques. The key sample preparation steps of homogenization, pretreatment, extraction, and purification are discussed as well as typical analytical methods. Consideration is given to application of these steps for particular sample matrixes to maximize yield, reduce inhibition effects, and minimize contamination. The choice of the most appropriate and valid methods for sample preparation from particular foods is discussed with respect to DNA analysis. Attention is also given to ease of use, cost, and generic applicability of the procedures.
Terry
Catherine F
CF
BioAnalytical Innovation Team, LGC, Teddington, Middlesex, UK. catherine.terry@lgc.co.uk
Harris
Neil
N
Parkes
Helen C
HC
D018556:Crops, Agricultural / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D030841:Food, Genetically Modified; D010940:Plant Proteins / Q000032:analysis; D030821:Plants, Genetically Modified / Q000235:genetics*
D018744:DNA, Plant; D010940:Plant Proteins