title: Effects of chronic caloric restriction on mitochondrial respiration in the ischemic reperfused rat heart.
abstract: Dietary restriction increases life span and delays the development of age-related diseases in rodents. We have recently demonstrated that chronic dietary restriction is beneficial on recovery of heart function following ischemia. We studied whether the metabolic basis of this benefit is associated with alterations in mitochondrial respiration. Male Wistar rats were assigned to an ad libitum-fed (AL) group and a food restricted (FR) group, in which food intake was reduced to 55% of the amount consumed by the AL group. Following an 8-month period of restricted caloric intake, isolated working hearts perfused with glucose and high levels of fatty acids were subjected to global ischemia followed by reperfusion. At the end of reperfusion, total heart mitochondria respiration was assessed in the presence of pyruvate, tricarboxylic acid intermediates, and palmitoylcarnitine. Recovery of heart function following ischemia was greater in FR hearts compared to AL hearts. Paralleling these changes in heart function was an increase in state 3 respiration with pyruvate. The respiratory control ratios in the presence of pyruvate and tricarboxylic acid intermediates were higher in FR hearts compared to AL hearts, indicating well-coupled mitochondria. Overall energy production, expressed as the ADP:O ratio and the oxidative phosphorylation rate, was also improved in FR hearts. Our results indicate that the beneficial effect of FR on recovery of heart function following ischemia is associated with changes in mitochondrial respiration.
authors.affiliation: Department of Physiology, Midwestern University, Glendale, AZ 85308, USA. tbrode@arizona.midwestern.edu
authors.forename: Tom L
authors.initials: TL
authors.lastname: Broderick
authors.forename: Terry
authors.initials: T
authors.lastname: Belke
authors.forename: William R
authors.initials: WR
authors.lastname: Driedzic
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D031204:Caloric Restriction; D002264:Carboxylic Acids / Q000378:metabolism; D019069:Cell Respiration; D004032:Diet; D005227:Fatty Acids / Q000008:administration & dosage; D005947:Glucose / Q000008:administration & dosage; D006321:Heart / Q000502:physiology*; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D010172:Palmitoylcarnitine / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002264:Carboxylic Acids; D005227:Fatty Acids; D010172:Palmitoylcarnitine; D000244:Adenosine Diphosphate; D019289:Pyruvic Acid; D005947:Glucose