title: Cytogenetic analysis of an asymmetric potato hybrid.
abstract: An asymmetric potato hybrid and its parental lines were cytogenetically examined. DAPI (4'-6-diamidino-2-phenylindole) staining was used to count chromosomes in all analysed lines and revealed the presence of minichromosomes in the hybrid genome. Fluorescent in situ hybridization (FISH) with rDNA sequence as a probe helped to determine the ploidy level of analysed lines and revealed that none of the minichromosomes contains rDNA repeats. CMA (chromomycin A3) band occurred to be a new chromosome marker that identifies potato chromosome No.1. It was possible to detect a deletion in one of four chromosomes No. 1 of the asymmetric potato hybrid. On the basis of these analyses a karyotype of the asymmetric hybrid was constructed.
authors.affiliation: Department of Plant Anatomy and Cytology, Silesian University, ul. Jagiellonska 28, 40-032 Katowice, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Srebniak
authors.forename: Ole
authors.initials: O
authors.lastname: Rasmussen
authors.forename: Jolanta
authors.initials: J
authors.lastname: Małuszyńska
mesh_terms: D004275:DNA, Ribosomal / Q000235:genetics; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D012091:Repetitive Sequences, Nucleic Acid; D011198:Solanum tuberosum / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal