[Simultaneous determination of five antioxidants in food by HPLC with fluorescence detection].
An HPLC method with fluorescence detection was developed for the determination of propyl gallate, nordihydroguaiaretic acid, butylated hydroxyanisole (2- and 3-tert-butyl-4-hydroxyanisole), tert-butylhydroquinone and octyl gallate in edible oils and foods. The antioxidants in edible oil were isolated directly with acetonitrile saturated with n-hexane. The antioxidants in food were extracted with ethyl acetate and the extract was concentrated under vacuum. They were isolated from the residue with acetonitrile saturated with n-hexane. The acetonitrile layer was centrifuged at 5,000 rpm for 10 min. The HPLC separation was performed on a Symmetry C18 column (3.5 microns, 4.6 mm i.d. x 150 mm) using a mixture of 5% acetic acid-acetonitrile-methanol (4:3:3, v/v/v) as the mobile phase and monitored by using a fluorescence detector with time programming. Sample peaks were identified by comparison of the fluorescence spectra with those of antioxidant standards. Average recoveries of fortified antioxidants at 100 micrograms/g were 72.1-99.6%. Coefficients of variation were 0.7-7.2%.
Oishi
Mitsuo
M
Tokyo Metropolitan Research Laboratory of Public Health: 3-24-1, Hyakunin-cho, Shinjyuku-ku, Tokyo 169-0073, Japan.
Matsuda
Toshiharu
T
Nojiri
Shuko
S
Saito
Kazuo
K
D000975:Antioxidants / Q000032:analysis*; D002079:Butter / Q000032:analysis; D002083:Butylated Hydroxyanisole / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004042:Dietary Fats, Unsaturated / Q000032:analysis; D005453:Fluorescence; D005504:Food Analysis / Q000379:methods*; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D006873:Hydroquinones / Q000032:analysis*; D008383:Margarine / Q000032:analysis; D009637:Masoprocol / Q000032:analysis*; D011435:Propyl Gallate / Q000032:analysis*
D000975:Antioxidants; D004042:Dietary Fats, Unsaturated; D006873:Hydroquinones; C016627:octyl gallate; D002083:Butylated Hydroxyanisole; D005707:Gallic Acid; D009637:Masoprocol; D002079:Butter; D008383:Margarine; D011435:Propyl Gallate; C018855:2-tert-butylhydroquinone