title: Selective removal of chymotrypsin using diphenyl alpha-aminoalkylphosphonate immobilized on sepharose gel.
abstract: A diphenyl alpha-aminoalkylphosphonate derivative, which is an irreversible inhibitor of chymotrypsin-like serine proteases, was immobilized on cyanogen bromide-activated Sepharose, and the selective binding of chymotrypsin to the obtained inhibitor-gel was evaluated using batch and column methods. Complete removal of chymotrypsin in an aqueous solution was done using the column method, while partial removal was done using the batch method.
authors.affiliation: Department of System Engineering of Materials and Life Science, Faculty of Engineering, Toyama University, Japan. shinono@eng.toyama-u.ac.jp
authors.forename: Shin
authors.initials: S
authors.lastname: Ono
authors.forename: Makiko
authors.initials: M
authors.lastname: Umezaki
authors.forename: Hajime
authors.initials: H
authors.lastname: Nabari
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Nakayama
authors.forename: Kumi
authors.initials: K
authors.lastname: Hasegawa
authors.forename: Satomi
authors.initials: S
authors.lastname: Sako
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Fujii
authors.forename: Isao
authors.initials: I
authors.lastname: Yamazaki
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Yoshimura
mesh_terms: D002918:Chymotrypsin / Q000737:chemistry / Q000302:isolation & purification*; D063065:Organophosphonates / Q000737:chemistry*; D012685:Sepharose / Q000737:chemistry*
chemical_list: D063065:Organophosphonates; D012685:Sepharose; D002918:Chymotrypsin