Molecular cloning and characterization of dullard: a novel gene required for neural development.
In a screen for genes expressed in neural tissues and pronephroi, we isolated a novel gene, named dullard. Dullard protein contains the C-terminal conserved domain of NLI-IF (Nuclear LIM Interactor-Interacting Factor), a protein whose function is not yet characterized. Dullard mRNA was maternally derived and localized to the animal hemisphere. At neurula stages, the expression was in neural regions and subsequently localized to neural tissues, branchial arches, and pronephroi. Using antisense morpholino oligonucleotide-mediated inhibition, we showed that dullard was required for neural development. The translational knock-down of dullard resulted in failure of neural tube development and the embryos consequently showed a reduction of head development. Expression of neural marker genes in dullard-inhibited embryos was also suppressed. These results suggest that dullard is necessary for neural development.
Satow
Reiko
R
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8654, Japan.
Chan
Te-chuan
TC
Asashima
Makoto
M
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000502:physiology*; D009420:Nervous System / Q000196:embryology* / Q000378:metabolism; D020320:Oligoribonucleotides, Antisense / Q000494:pharmacology; D010749:Phosphoprotein Phosphatases; D012333:RNA, Messenger / Q000096:biosynthesis; D016415:Sequence Alignment; D029867:Xenopus Proteins / Q000235:genetics* / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology / Q000378:metabolism
D009419:Nerve Tissue Proteins; D020320:Oligoribonucleotides, Antisense; D012333:RNA, Messenger; D029867:Xenopus Proteins; C499225:CTDNEP1 protein, human; D010749:Phosphoprotein Phosphatases; C462021:ctdnep1 protein, Xenopus