Bioassay-directed chemical analysis of River Elbe surface water including large volume extractions and high performance fractionation.
A bioassay-directed fractionation and identification (toxicity identification evaluation procedure) was performed on extracts of 10 1 River Elbe water samples. The experimental method included a SDB-1 solid phase extraction followed by RP-HPLC fractionation and subfractionation. Chemical analysis by GC-MS as well as acute toxicity testing using a luminescent bacteria assay were conducted in the respective fractions. Many substances were identified, among which were pesticides and pharmaceuticals, but many compounds remained unknown.
Reineke
N
N
Institute of Organic Chemistry, University of Hamburg, Germany.
Bester
K
K
Hühnerfuss
H
H
Jastorff
B
B
Weigel
S
S
D002851:Chromatography, High Pressure Liquid; D064420:Drug-Related Side Effects and Adverse Reactions; D008401:Gas Chromatography-Mass Spectrometry; D005858:Germany; D007928:Lethal Dose 50; D010575:Pesticides / Q000032:analysis / Q000633:toxicity; D004364:Pharmaceutical Preparations / Q000032:analysis; D014733:Vibrio; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity*
D010575:Pesticides; D004364:Pharmaceutical Preparations; D014874:Water Pollutants, Chemical