title: Molecular Design and Bio-activity Analysis of a t-PA Derivative (t-PA S165W).
abstract: In order to obtain tissue-type plasminogen activator (t-PA) mutant with enhanced affinity for fibrin, redesigning t-PA strategies were proposed by computer molecular designing technology, and the re-designed t-PA derivative, t-PA S165W, was expressed in CHO cells. The bio-activities and affinity for fibrin of the expression product were tested. No apparent changes were observed between t-PA S165W and wild type t-PA. Therefore, single site mutation of the t-PA could not lead to enhanced affinity for fibrin of t-PA.
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. huangcf@nic.bmi.ac.cn
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Guo-Li
authors.initials: GL
authors.lastname: Wang
authors.forename: Pei-Tang
authors.initials: PT
authors.lastname: Huang
authors.forename: Cui-Fen
authors.initials: CF
authors.lastname: Huang