Production of cyclopiazonic acid by Penicillium commune isolated from dry-cured ham on a meat extract-based substrate.
Penicillium commune, a mold frequently found on dry-cured meat products, is able to synthesize the mycotoxin cyclopiazonic acid (CPA). To evaluate the hazard due to CPA on such foods, the ability of P. commune to grow and produce CPA at water activities (a(w)) in the range of 0.99 to 0.90 with a meat extract-based medium from 12 to 30 degrees C was determined. CPA was quantified by high-pressure liquid chromatography and mass spectrometry. P. commune was able to grow at every a(w) and temperature tested. The optimal environmental conditions for growth were 20 to 25 degrees C, at 0.97 to 0.96 a(w), but the highest amount of CPA was produced at 30 degrees C, 0.96 a(w). No direct correlation between growth rate and CPA production was assessed. Temperature seems to be the most important factor influencing CPA production. However, there was an interaction between temperature and a(w) that significantly (P < 0.001) affected growth and CPA production. An a(w) of 0.90 had a marked effect, depressing growth and CPA production. Meat extract-based medium proved to be an appropriate substrate for CPA biosynthesis by P. commune under a wide range of conditions.
Sosa
María J
MJ
Higiene de los Alimentos, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
Córdoba
Juan J
JJ
Díaz
Carmen
C
Rodríguez
Mar
M
Bermúdez
Elena
E
Asensio
Miguel A
MA
Núñez
Félix
F
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004791:Enzyme Inhibitors / Q000378:metabolism; D005516:Food Microbiology; D007211:Indoles / Q000032:analysis / Q000378:metabolism*; D013058:Mass Spectrometry; D008461:Meat Products / Q000382:microbiology*; D010407:Penicillium / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013552:Swine; D013696:Temperature; D014867:Water
D003470:Culture Media; D004791:Enzyme Inhibitors; D007211:Indoles; D014867:Water; C000543:cyclopiazonic acid