Uncoupling activation-induced modulation of CD16 and CD69 in CD56+ cells during AIDS.
The immune system of HIV+ patients is chronically activated, which has been associated with a detrimental effect on both innate and acquired immunity during AIDS. We analyzed the expression and modulation of the triggering markers CD69 and CD16 in CD56+ cells from 18 asymptomatic HIV+ individuals and 8 AIDS patients, compared with 21 seronegative subjects. We observed a diminished PMA-induced CD16 downregulation in AIDS patients (p<0.01), associated with low numbers of CD4+ cells (p<0.02). Furthermore, an enhanced unstimulated expression of CD69 in asymptomatic HIV+ patients (p<0.05) was shown. AIDS patients could not efficiently upregulate PHA-dependent CD69 expression (p<0.05), which correlated with low CD4+ counts (p< 0.05). These abnormalities in CD16 and CD69 modulation were recorded in patients under highly active antiretroviral therapy (HAART). Our results demonstrate an altered modulation of two functionally relevant receptors in CD56+ cells from AIDS patients, contributing to our understanding of the immunopathogeny of NK cell dysfunction during disease progression.
Barboza
José M
JM
Instituto de Immunología Clínica, Facultad de Medicina, Universidad de Los Andes, Mérida-Venezuela.
Salmen
Siham
S
Cova
José A
JA
Albarrán
Benibelks
B
Goncalves
Loredana
L
Borges
Lérida
L
Hernández
Manuel
M
Berrueta
Lisbeth
L
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000096:biosynthesis*; D019002:CD56 Antigen / Q000276:immunology*; D015536:Down-Regulation; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000276:immunology; D017452:Receptors, IgG / Q000032:analysis / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000276:immunology; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D019002:CD56 Antigen; D037181:Lectins, C-Type; D010835:Phytohemagglutinins; D017452:Receptors, IgG; D013755:Tetradecanoylphorbol Acetate; D000099192:CD69 Antigens