title: The cytoskeleton and the secretory pathway are not involved in targeting the cowpea mosaic virus movement protein to the cell periphery.
abstract: The movement protein (MP) of cowpea mosaic virus (CPMV) forms tubules on infected protoplasts and through plasmodesmata in infected plants. In protoplasts the MP fused to GFP (MP-GFP) was shown to localize in peripheral punctate structures and in long tubular structures extending from the protoplast surface. Using cytoskeletal assembly inhibitors (latrunculin B and oryzalin) and an inhibitor of the secretory pathway (brefeldin A), targeting of the MP to the peripheral punctate structures was demonstrated not to be dependent on an intact cytoskeleton or functional secretion pathway. Furthermore it was shown that a disrupted cytoskeleton had no effect on tubule formation but that the addition of brefeldin A severely inhibited tubule formation. The results presented in this paper suggest a role for a plasma membrane host factor in tubule formation of plant viral MPs.
authors.affiliation: Laboratory of Molecular Biology, Wageningen University, The Netherlands.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Pouwels
authors.forename: Gerard N M
authors.initials: GN
authors.lastname: Van Der Krogt
authors.forename: Jan
authors.initials: J
authors.lastname: Van Lent
authors.forename: Ton
authors.initials: T
authors.lastname: Bisseling
authors.forename: Joan
authors.initials: J
authors.lastname: Wellink
mesh_terms: D020126:Brefeldin A / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D017798:Comovirus / Q000378:metabolism*; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004136:Dinitrobenzenes / Q000494:pharmacology; D007887:Fabaceae / Q000821:virology; D006056:Golgi Apparatus / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006540:Herbicides / Q000494:pharmacology; D008164:Luminescent Proteins; D053830:Plant Viral Movement Proteins; D011523:Protoplasts / Q000378:metabolism*; D022142:Secretory Vesicles / Q000187:drug effects / Q000378:metabolism; D013424:Sulfanilamides; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D004136:Dinitrobenzenes; D006540:Herbicides; D008164:Luminescent Proteins; D053830:Plant Viral Movement Proteins; D013424:Sulfanilamides; D013844:Thiazoles; D053778:Thiazolidines; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D020126:Brefeldin A; C012465:oryzalin; C037068:latrunculin B