Proliferation and morphological transformation of RMK cells exposed to hydroquinine containing ionomers.
Recent research in our laboratories has been directed towards the development of ionomeric polymers and monomers for use in biomedical applications such as adhesives, drug delivery matrices and tissue scaffolds. The chemical Hydroquinone (HQ) aids as a stabilizer and represents a major component in the development of the ionomers. However, hydroquinone in high concentration has the potential to initiate carcinogenic effects on cells. The curing reactions are based on free radical chemistry that require a radical scavenger, ascorbic acid (Asc) to adjust working and setting times and shelf-life stability. The few studies published on HQ have suggested that high dosages of HQ may stimulate apoptosis as well as an increased cellular leakage, however the effect of HQ on the biocompatability is unknown. Therefore the objectives of this study were to measure the functional capacity, cell proliferation and structural integrity of Rhesus monkey kidney epithelial (RMK) cells exposed to ionomer formulations containing 4 different levels of HQ. A total of 90 tubes of RMK (40,000 cells per tube) cells were divided equally into five equal groups. Group I served as a control and group II-V were subjected to ionomers containing 0, 500, 1000, and 2000 ppm HQ. Cell numbers, morphology, cellular and supermatant MDA levels, and total protein analysis were performed. The results suggest: (I) All ionomer groups increased cellular proliferation except for the 2000 ppm HQ group, (II) MDA levels were increased in cells containing 2000 ppm HQ at 24 hours; and 0 ppm at 48 hours. It may be concluded that HQ concentrations over 1000 ppm may adversely affect biocompatability.
Harvey
Veronica
V
University of Mississippi Medical Center, Jackson, Mississippi 39216, USA.
Benghuzzi
Hamed
H
Tucci
Michell
M
Puckett
Aaron
A
Cason
Zelma
Z
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000166:cytology* / Q000378:metabolism; D008253:Macaca mulatta; D008315:Malondialdehyde / Q000378:metabolism; D011802:Quinidine / Q000031:analogs & derivatives* / Q000633:toxicity*
D008315:Malondialdehyde; C014486:hydroquinidine; D011802:Quinidine