Role of CD4+ and CD8+ T lymphocytes in the protection of mice against Encephalitozoon intestinalis infection.
Severe combined immunodeficient (SCID) mice reconstituted with spleen cells from naive adult BALB/c mice were completely resistant to peroral infection with Encephalitozoon intestinalis (Calli, Kotler and Orenstein, 1993) Canning, Field, Hing and Marriott, 1994, whereas control, non-reconstituted SCID mice succumbed to the infection. The role of T-lymphocyte subpopulations in the protection against peroral E. intestinalis infection was studied in adoptive transfer experiments using SCID mice. SCID mice reconstituted with both CD4+ and CD8+ T-lymphocyte-depleted splenocytes succumbed to the peroral route of infection. In contrast, SCID mice reconstituted with either CD4+-depleted or CD8+ T-lymphocyte-depleted splenocytes completely resolved the infection. This indicates that CD4+ and CD8+ T-lymphocyte subpopulations play a substantive role in protection against peroral infection with the microsporidian, E. intestinalis.
Salát
Jirí
J
Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, Branisovská 31, 37005 Ceské Budejovice, Czech Republic. george@paru.cas.cz
Braunfuchsová
Pavlína
P
Kopecký
Jan
J
Ditrich
Oleg
O
D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D004195:Disease Models, Animal; D016819:Encephalitozoon / Q000276:immunology / Q000648:ultrastructure; D016890:Encephalitozoonosis / Q000276:immunology* / Q000517:prevention & control*; D007167:Immunotherapy; D008212:Lymphocyte Depletion; D017710:Lymphocyte Transfusion; D051379:Mice; D016513:Mice, SCID; D008854:Microscopy, Electron