Presence of phenol UDP-glucuronosyltransferase in bovine alveolar macrophages and bronchial epithelial cells.
Pulmonary organ and cells are the primary target of atmospheric pollutants. Phenol UDP-glucuronosyltransferase (UGT) was found in bovine alveolar macrophage cells by immunohistochemical staining and also was observed in bronchial epithelial cells of the lung. A high level of activity of UGT, which is one of the phase II drug-metabolizing enzymes, toward 1-naphthol was observed in the microsomes of both cell types. By Western blotting analysis, a 54-kDa band was detected in alveolar macrophage cells and in bovine lung using polyclonal antibodies against a purified rat UGT, which catalyze the glucuronidation of various phenolic xenobiotics such as 1-naphthol and have the same molecular mass (54 kDa). Reverse transcriptase-polymerase chain reaction (RT-PCR) amplified the common cDNA region in UGT1A subfamily isoforms, indicating that UGT1A subfamily isoform was expressed in alveolar macrophages and in bronchial epithelial cells of the lung. These results suggest that phenol UGT act as a primary barrier against various phenolic chemicals in the lung.
Yamashiki
Naoko
N
Department of Biology, Faculty of Environmental System, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
Yokota
Hiroshi
H
Sakamoto
Maiko
M
Yuasa
Akira
A
D000818:Animals; D015153:Blotting, Western; D001980:Bronchi / Q000201:enzymology*; D002417:Cattle; D004847:Epithelial Cells / Q000201:enzymology*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D016676:Macrophages, Alveolar / Q000201:enzymology*; D018613:Microscopy, Confocal; D009284:Naphthols / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015262:Xenobiotics / Q000378:metabolism
D007527:Isoenzymes; D009284:Naphthols; D015262:Xenobiotics; C029350:1-naphthol; D014453:Glucuronosyltransferase