title: Gene expression profiling of cell lines derived from T-cell malignancies.
abstract: The expression profiles of eight cell lines derived from T-cell malignancies were compared to CD4-positive T-cells using cDNA microarray technology. Unsupervised hierarchical clustering of 4364 genes demonstrated substantial heterogeneity resulting in four distinct groups. While no genes were found to be uniformly up- or down-regulated across all cell lines, we observed 111 over-expressed genes (greater than two-fold) and 1118 down-regulated genes (greater than two-fold) in the lymphomas as a group when compared to CD4-positive T-cells. These included genes involved in cytokine signaling, cell adhesion, cytoskeletal elements, nuclear transcription factors, and known oncogenes and tumor suppressor genes. Quantitative fluorescent reverse transcription-polymerase chain reaction analysis demonstrated 70% concordance with the microarray results. While freshly isolated malignant cells may differ in their individual expression patterns relative to established cell lines from the same diagnoses, we feel that the variety of different lymphocytic cell lines that we examined provides a representative picture of the molecular pathogenesis of T-cell malignancies.
authors.affiliation: Department of Pathology, University of Utah Health Sciences Center, 50 North Medical Drive, Salt Lake City 84132, USA.
authors.forename: G Chris
authors.initials: GC
authors.lastname: Fillmore
authors.forename: Zhaosheng
authors.initials: Z
authors.lastname: Lin
authors.forename: Sandra D
authors.initials: SD
authors.lastname: Bohling
authors.forename: Ryan S
authors.initials: RS
authors.lastname: Robetorye
authors.forename: Chan-Hwan
authors.initials: CH
authors.lastname: Kim
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Jenson
authors.forename: Kojo S J
authors.initials: KS
authors.lastname: Elenitoba-Johnson
authors.forename: Megan S
authors.initials: MS
authors.lastname: Lim
mesh_terms: D015496:CD4-Positive T-Lymphocytes; D005456:Fluorescent Dyes; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D019169:Jurkat Cells; D016399:Lymphoma, T-Cell / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes; D014407:Tumor Cells, Cultured
chemical_list: D005456:Fluorescent Dyes