Production of lytic enzymes by Trichoderma spp. and their effect on the growth of phytopathogenic fungi.
The production of beta-1,3-glucanases and chitinases by three strains of Trichoderma in submerged cultures was determined. The synthesis of enzymes was induced by cell wall biopolymers of phytopathogenic fungi (Botrytis cinerea, Fusarium culmorum and F. oxysporum). T. hamatum produced the highest beta-1,3-glucanase activity; the most effective inducer of enzyme synthesis was the biomass of F. oxysporum. All examined strains of Trichoderma inhibited phytopathogen growth in biotic tests. The diffusion tests showed that the lytic enzymes take part in growth inhibition of phytopathogenic fungi.
Witkowska
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Department of Biotechnology and Food Microbiology, Agricultural University of Wrocław, 50-375 Wrocław, Poland.
Maj
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D020171:Botrytis / Q000187:drug effects*; D002688:Chitinases / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects*; D043326:Glucan 1,3-beta-Glucosidase; D008826:Microbial Sensitivity Tests; D010935:Plant Diseases / Q000382:microbiology; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology*; D001617:beta-Glucosidase / Q000494:pharmacology* / Q000502:physiology
D002688:Chitinases; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase