[Screening and characterization of Schistosoma japonicum reinfection-related cDNA clones].
OBJECTIVE
To obtain cDNA clones coding for S. japonicum (Sj) reinfection-related specific immunologic molecules for vaccination or diagnosis.


METHODS
An adult S. japonicum cDNA library, of which the cloning efficiency is 3.13 x 10(6), with directional inserts in the vector lambda gt11, was screened with pooled serum from 43 reinfected human cases in a schistosomiasis-endemic area with high level of Sj specific-lgG4 antibodies. Primers located several bases away from the insert position on the lambda gt11 arm were used to amplify the insert fragments by standard PCR. SILVER SEQUENCE (PCR-silver) DNA sequencing system was then used to sequence selected positive clones with relatively large inserts and homology comparison with GenBank database was also carried out.


CONCLUSIONS
Eleven phage plaques were defined as positive out of the 8.6 x 10(4) phage plaques immunoscreened by human specific lgG4 antibody. GenBank database retrieval showed that 4 new Sj gene clones including Sj reinfection-related specific protein-encoding gene and Sj mitochondrial protein-coding gene have been obtained.
Wu
H
H
Laboratory of Molecular Immuno-Parasitology, Nanjing Medical University, Nanjing, Jiangsu, 210029.
Wang
R
R
Chen
S
S
Su
C
C
Zhang
Z
Z
Wu
G
G
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D015723:Gene Library; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics*; D012008:Recurrence; D012554:Schistosomiasis japonica / Q000235:genetics*
D000909:Antibodies, Helminth; D015801:Helminth Proteins; D024101:Mitochondrial Proteins