Expression pattern of a mini human PrP gene promoter in transgenic mice.
The prion protein is central to the pathogenesis of prion diseases, although its exact function remains unclear. Although transgenic mice have been widely utilised in prion research, their PrP expression patterns have not been characterised in detail. We have studied the developmental temporal and spatial expression of a 214-bp mini human PrP promoter in transgenic mice. Transgene expression is first detected at embryonic day 12.5, a day earlier than previously reported for endogenous mouse gene by in situ hybridization. The general expression pattern closely mirrors that of the endogenous mouse PrP gene, such that this small and clearly defined transgene cassette can replace the need to use large cosmid based vectors for transgenetic modeling of human and animal prion disease.
Asante
E A
EA
MRC Prion Unit, Department of Neurodegenerative Disease, Institute of Neurology, University College, Queen Square, London, WCIN 3BG, United Kingdom.
Gowland
I
I
Linehan
J M
JM
Mahal
S P
SP
Collinge
J
J
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D004622:Embryo, Mammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D011247:Pregnancy; D011328:Prions / Q000096:biosynthesis* / Q000235:genetics*; D011401:Promoter Regions, Genetic; D019076:Transgenes
D011328:Prions