Demonstration of expression of mRNA for particular AMPA and kainate receptor subunits in immature and mature cultured rat calvarial osteoblasts.
Reverse transcription polymerase chain reaction revealed expression of mRNA for particular subunits of ionotropic glutamate receptors (iGluR) in primary cultures of rat calvarial osteoblastic cells under immature to mature states. These included GluR3, KA1 and KA2 subunits, in addition to NR1 and NR2D subunits. These results suggest that glutamate may play an unidentified role in mechanisms associated with cellular development through particular subunits of iGluR in rat calvarial osteoblasts.
Hinoi
Eiichi
E
Department of Molecular Pharmacology, Kanazawa University Faculty of Pharmaceutical Sciences, 13-1 Takara-machi, Kanazawa, Ishikawa, Japan .
Fujimori
Sayumi
S
Takemori
Akihiro
A
Kurabayashi
Hiroaki
H
Nakamura
Yoichi
Y
Yoneda
Yukio
Y
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000096:biosynthesis* / Q000235:genetics; D018092:Kainic Acid Receptors / Q000096:biosynthesis* / Q000235:genetics; D012886:Skull / Q000166:cytology* / Q000378:metabolism*
D012333:RNA, Messenger; D018091:Receptors, AMPA; D018092:Kainic Acid Receptors