Differentiation of porcine Brachyspira species by a novel nox PCR-based restriction fragment length polymorphism analysis.
A novel PCR-based restriction fragment length polymorphism analysis of the Brachyspira nox gene was developed. The restriction patterns for Brachyspira hyodysenteriae, B. pilosicoli, B. intermedia, B. murdochii, and B. innocens were highly distinct with two restriction endonucleases only. The assay proved to be user-friendly and robust.
Rohde
Judith
J
Institut für Mikrobiologie und Tierseuchen, Tieraerztliche Hochschule Hannover, Hannover, Germany. Jurohde@gmx.de
Rothkamp
Anja
A
Gerlach
Gerald F
GF
D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D013144:Spirochaetales / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D013552:Swine / Q000382:microbiology*
D017931:DNA Primers; D004269:DNA, Bacterial