Ecdysone-controlled expression of transgenes.
Inducible expression systems show great potential for use in human gene therapy and systems based on insect ecdysone receptors are particularly promising candidates. This article describes such systems and reviews actual and potential uses of ecdysone-controlled transgenes in vitro and in vivo. The ligand specificity of ecdysone receptor-based systems is considered, along with the safety and efficacy of the ecdysteroid and non-steroidal compounds used to activate them.
Graham
Lloyd D
LD
CSIRO Molecular Science, Sydney Laboratory, PO BOX 184, North Ryde, NSW 1670, Australia. lloyd.graham@csiro.au
D000818:Animals; D004440:Ecdysone / Q000009:adverse effects / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008024:Ligands; D013256:Steroids / Q000009:adverse effects / Q000494:pharmacology; D019076:Transgenes / Q000187:drug effects*
D008024:Ligands; D013256:Steroids; D004440:Ecdysone