Gene expression profile for schizophrenia: discrete neuron transcription patterns in the entorhinal cortex.
BACKGROUND
Several lines of evidence indicate the altered function of the temporal lobe, including the hippocampus and entorhinal cortex (EC), is associated with schizophrenia. We used single-cell gene expression technologies to assess coordinate changes in the expression of multiple genes, including neuronal signaling and synaptic-related markers in EC layer II stellate neurons.


METHODS
We used a single-neuron microdissection technique coupled with linear antisense RNA amplification and high density/candidate gene arrays to assess coordinate changes in gene expression. The expression and relative abundance of more than 18,000 messenger RNAs were assessed from EC layer II stellate neurons from postmortem samples of schizophrenic and age-matched control brains. Results of this initial screen were used to perform a more specific secondary messenger RNA screen for each subject.


RESULTS
Data disclosed marked differences in expression of various G-protein-coupled receptor-signaling transcripts, glutamate receptor subunits, synaptic proteins, and other transcripts. Results of secondary screening showed significant decreases in levels of G-protein subunit i(alpha)1, glutamate receptor 3, N-methyl-D-aspartate receptor 1, synaptophysin, and sensory nerve action potentials 23 and 25 in the stellate neurons of schizophrenic patients. We observed down-regulation of phospholemman (a phosphoprotein associated with anion channel formation) messenger RNA and protein levels in layer II/III stellate neurons in the population with schizophrenia.


CONCLUSIONS
These results provide a preliminary expression profile of schizophrenia in defined neuronal populations. Understanding the coordinated involvement of multiple genes in human disease provides insight into the molecular basis of the disease and offers new targets for pharmacotherapeutic intervention.
Hemby
Scott E
SE
Department of Pharmacology, Yerkes Regional Primate Research Center, Neuroscience Division, Emory University School of Medicine, Atlanta, GA, USA.
Ginsberg
Stephen D
SD
Brunk
Brian
B
Arnold
Steven E
SE
Trojanowski
John Q
JQ
Eberwine
James H
JH
D000368:Aged; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D018728:Entorhinal Cortex / Q000166:cytology / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D008040:Genetic Linkage; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D017470:Receptors, Glutamate / Q000235:genetics / Q000378:metabolism; D012559:Schizophrenia / Q000235:genetics / Q000378:metabolism*; D013569:Synapses / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
D002352:Carrier Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D017470:Receptors, Glutamate; C100493:SNAP23 protein, human; C069141:phospholemman