title: Levels of tissue inhibitor of metalloproteinases-1 in blood transfusion components.
abstract: Blood transfusion during surgery for solid tumors may reduce patient survival because of various bioactive substances present in blood preparations. The anti-proteolytic protein tissue inhibitor of metalloproteinases-1 (TIMP-1) present in large quantities in platelets has been shown to stimulate cell growth and to inhibit apoptosis and may therefore be considered to influence tumor progression. We measured TIMP-1 levels in blood transfusion preparations. especially in platelet-containing preparations, before and after leucofiltration and at different time-points during storage. The mean TIMP-1 levels in whole blood (WB) and platelet-rich plasma (PRP) were slightly reduced by leucofiltration; WB: 41.6 microg/L versus 34.9 microg/L. PRP: 139.8 microg/L versus 127.2 microg/L. However, with prestorage leucofiltration. TIMP-1 levels in buffy-coat-derived platelet (BCP) pools were significantly reduced from 134.2 microg/L to 102.2 microg/L (p=0.0013). In saline-adenine-glucose-mannitol (SAG-M) blood preparations in which the platelet content is reduced by more than 99%,. TIMP-1 could not be detected. Extracellular TIMP-1 accumulated significantly in non-filtered WB and in aferesis platelet concentrates (APC), but TIMP-1 was at no time detectable in SAG-M blood during storage. In conclusion. TIMP-1 is present in various platelet-containing blood preparations, but not in platelet-free preparations such as SAG-M, indicating that most of the TIMP-1 measured in blood preparations originates from platelets. Furthermore, TIMP-1 levels increased during storage in preparations containing platelets. which suggests a continuous disintegration of platelets. These data imply that information on preoperative blood transfusions should be taken into account when evaluating plasma TIMP-1 levels in patients.
authors.affiliation: The Finsen Laboratory, Rigshospitalet, Copenhagen, Denmark.
authors.forename: M N
authors.initials: MN
authors.lastname: Holten-Andersen
authors.forename: N
authors.initials: N
authors.lastname: Brunner
authors.forename: I J
authors.initials: IJ
authors.lastname: Christensen
authors.forename: V
authors.initials: V
authors.lastname: Jensen
authors.forename: H J
authors.initials: HJ
authors.lastname: Nielsen
mesh_terms: D016913:Blood Component Transfusion; D001793:Blood Preservation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009369:Neoplasms / Q000097:blood* / Q000628:therapy*; D017713:Platelet Transfusion; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis / Q000097:blood*
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1