title: Cutaneous late phase reaction in adult atopic dermatitis patients with high serum IgE antibody to Dermatophagoides farinae: correlation with IL-5 production by allergen-stimulated peripheral blood mononuclear cells.
abstract: It is known that, in patients of allergic asthma and rhinitis, the late-phase reaction (LPR) occurs 6-12 h after allergen challenge, but there are few reports concerning cytokine production in the cutaneous LPR in atopic dermatitis (AD). We report here the results of our study on the relationship between the cutaneous LPR and the production of cytokines such as IL-4, IL-5, IL-2 and IFN-gamma by peripheral blood mononuclear cells (PBMC) of AD patients. We selected 29 pure AD patients with no history of atopic airway diseases who showed high serum IgE antibody against Dermatophagoides farinae and performed skin prick testing with three different antigens and observed the resultant cutaneous reactions in 23 of the AD patients. Furthermore, we measured the cytokine production by the cultured PBMC under the stimulation of the antigens and compared it with the results of the skin tests. 13 (57%) of these 23 AD patients demonstrated positive LPR in response to D. farinae, and the mean concentration of IL-5 produced by PBMC was higher in these LPR-positive AD patients compared to the LPR-negative ones. Additionally, we noticed that there was a positive correlation between the mean diameter of the erythema of LPR and the level of IL-5 production by PBMC in the LPR-positive patients. We suggest that there are at least two groups in AD patients, i.e. LPR-positive and LPR-negative ones. The observation of LPR can be an important and practical way to classify AD patients into subgroups, which may enable us to regard IL-5 or eosinophils as a target for treatment.
authors.affiliation: Department of Dermatology, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.
authors.forename: Mikiko
authors.initials: M
authors.lastname: Okada
authors.forename: Tadashi
authors.initials: T
authors.lastname: Terui
authors.forename: Motoko
authors.initials: M
authors.lastname: Honda
authors.forename: Misako
authors.initials: M
authors.lastname: Tanaka
authors.forename: Rika
authors.initials: R
authors.lastname: Chikama
authors.forename: Nobuko
authors.initials: N
authors.lastname: Tabata
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takahashi
authors.forename: Hachiro
authors.initials: H
authors.lastname: Tagami
mesh_terms: D000328:Adult; D039741:Antigens, Dermatophagoides; D016207:Cytokines / Q000096:biosynthesis; D003876:Dermatitis, Atopic / Q000175:diagnosis / Q000503:physiopathology*; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000175:diagnosis / Q000503:physiopathology; D006969:Hypersensitivity, Immediate / Q000175:diagnosis / Q000503:physiopathology; D007073:Immunoglobulin E / Q000097:blood*; D015848:Interleukin-5 / Q000096:biosynthesis; D008297:Male; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D012016:Reference Values; D012867:Skin / Q000503:physiopathology*; D012882:Skin Tests; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
chemical_list: D039741:Antigens, Dermatophagoides; D016207:Cytokines; D006023:Glycoproteins; D015848:Interleukin-5; D007073:Immunoglobulin E