Comparison of the expression patterns of two LIM-homeodomain genes, Lhx6 and L3/Lhx8, in the developing palate.
OBJECTIVE
To compare and contrast the gene expression of two LIM-homeobox type transcription factors, Lhx6 and L3/Lhx8, in secondary palate formation.


METHODS
In situ hybridization histochemistry with digoxygenin (DIG) labelled cRNA probes specific for Lhx6 and L3/Lhx8.


METHODS
Serial cryo-sections of embryonic day (E)13.5, 14.5, and 15.5 mice (C57BL/6).


METHODS
Comparison of the signal intensities of NBT/BCIP precipitate by alkaline phosphatase conjugated anti-DIG antibody.


RESULTS
From E13.5 to E15.5, Lhx6 and L3/Lhx8 signals are detected in palatal mesenchyme, but the L3/Lhx8 signal is much more intense than the Lhx6 signal. In palatal epithelium, covering the mesenchyme, Lhx6 mRNA is transiently expressed at E14.5, while L3/Lhx8 mRNA expression is never detected throughout the development.


CONCLUSIONS
Lhx6 and L3/Lhx8 functions may be partially redundant in the mesenchyme of the secondary palate, but not in the palatal epithelium.
Zhang
Y
Y
Department of Anesthesiology, School of Medicine, Fukushima Medical University, Japan.
Mori
T
T
Takaki
H
H
Takeuch
M
M
Iseki
K
K
Hagino
S
S
Murakawa
M
M
Yokoya
S
S
Wanaka
A
A
D000818:Animals; D002972:Cleft Palate / Q000196:embryology*; D005314:Embryonic and Fetal Development; D004848:Epithelium / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox / Q000502:physiology; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D060850:LIM-Homeodomain Proteins; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins; D021362:Palate, Hard / Q000196:embryology*; D015347:RNA Probes; D014157:Transcription Factors
D018398:Homeodomain Proteins; C498504:LHX6 protein, mouse; C112960:LIM homeobox protein 8; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D015347:RNA Probes; D014157:Transcription Factors