Utilization of central disk of blastoderm and germinal crescent region for production of interspecific germline chimera between chicken and quail.
OBJECTIVE
The production of interspecific germline chimeras between chicken and quail were attempted employing the dissociated cells derived from the blastodermal central disk (stage X) and the germinal crescent region of embryo (stage 7-8).


METHODS
The central disk (CD) of the area pellucida in chicken blastoderm (stage X) and the germinal crescent region (GCR) of embryo (stage 7-8) were dispersed and injected into the subgerminal cavity of quail blastoderm (stage X). Injected eggs were incubated for 7 days or to hatching. The donor chicken DNA was detected by the polymerase chain reaction.


RESULTS
In day-7 embryos, chicken DNA was detected in 5 gonads and 9 brains from 53 survived embryos received chicken CD cells, and 1 gonads and 6 brains from 27 survived embryos received chicken GCR. Chicken DNA was also detected from the semen of one adult male hatched from eggs received chicken GCR cells.


CONCLUSIONS
CD and GCR cells as the donors showed the possibility to produce the interspecific germline chimera, but further studies are needed to make necessary improvement.
Soh
Tomoki
T
Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan. soht@agr.kyushu-u.ac.jp
Inoue
Yoshie
Y
Xi
Yong-Mei
YM
Kato
Yukio
Y
Hattori
Masa-Aki
MA
D000818:Animals; D001483:Base Sequence; D001756:Blastoderm / Q000502:physiology* / Q000648:ultrastructure; D001921:Brain / Q000196:embryology; D001923:Brain Chemistry; D002642:Chick Embryo / Q000502:physiology*; D002645:Chickens; D002678:Chimera; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000502:physiology*; D005260:Female; D018095:Germ-Line Mutation / Q000502:physiology*; D008297:Male; D010047:Ovalbumin / Q000235:genetics*; D010053:Ovary / Q000196:embryology; D016133:Polymerase Chain Reaction; D011784:Quail; D013737:Testis / Q000196:embryology
D017931:DNA Primers; D018076:DNA, Complementary; D010047:Ovalbumin