[Effect of pertussis bacilli on the release of excitatory amino acids in brain slice of rats].
OBJECTIVE
To investigate the effect of pertussis bacilli on the release of excitatory amino acids (EAAs) in rat-brain slice and to know whether MK-801(dizocipine) protects brain tissue from excitotoxicity.


METHODS
SD rats were randomly divided into 3 groups: 1. Normal slice cultured group(NC); 2. 10% pertussis bacilli(PB) slice cultured group(PBC); 3. 10% PB slice cultured group after pretreatment with MK-801. The release of EAAs from brain slices was measured by high performance liquid chromatography.


RESULTS
The concentrations of glutamate(Glu 29.53 +/- 8.00), aspartate(Asp 23.74 +/- 5.79) and LDH(38.03 +/- 8.45) were significantly higher in PBC group than those(Glu 16.46 +/- 4.00, Asp 15.27 +/- 5.31, LDH 23.10 +/- 5.11) in MPBC group and (Glu 16.09 +/- 3.74, Asp 11.47 +/- 2.51, LDH 25.82 +/- 4.50) NC group.


CONCLUSIONS
Pertussis bacilli may elicit a large increase of extracellular EAAs and LDH in vitro, while MK-801 protects brain tissue from excitotoxicity.
Yin
F
F
Department of Pediatrics, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Yang
Y
Y
Yu
P
P
D000818:Animals; D001886:Bordetella pertussis / Q000502:physiology*; D001921:Brain / Q000378:metabolism*; D018846:Excitatory Amino Acids / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D018846:Excitatory Amino Acids