Detection of beta-subunit human chorionic gonadotropin mRNA in the peripheral blood of patients with nonmetastatic gestational trophoblastic disease.
OBJECTIVE
The aim of this study was to detect beta-subunit human chorionic gonadotropin (beta(h)CG) mRNA in the peripheral blood samples of patients with nonmetastatic gestational trophoblastic disease (GTD) undergoing hysterectomy.


METHODS
Heparinized peripheral blood samples were obtained from four patients with nonmetastatic GTD before, during, and after hysterectomy. The beta(h)CG mRNA expression was examined by reverse transcriptase-polymerase chain reaction using beta(h)CG primers. The expression of beta(h)CG mRNA was quantified using a densitometer.


RESULTS
Beta(h)CG expression was detected in all patients before and during hysterectomy. The expression of beta(h)CG mRNA during operation was so high that it could not be quantified using densitometer. The expression decreased rapidly after operation.


CONCLUSIONS
Disseminated trophoblastic cells are present in the peripheral blood even in cases without metastasis. Trophoblastic cells circulating in the peripheral blood can be reduced by surgical intervention.
Suzuka
Kiyomi
K
Department of Obstetrics and Gynecology, Chiba University School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. suzukak@med.m.chiba-u.ac.jp
Matsui
Hideo
H
Iitsuka
Yoshinori
Y
Sekiya
Souei
S
D000328:Adult; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D007044:Hysterectomy; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000097:blood*; D014328:Trophoblastic Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D014407:Tumor Cells, Cultured
D018997:Chorionic Gonadotropin, beta Subunit, Human; D012333:RNA, Messenger