title: Successful boosting of a DNA measles immunization with an oral plant-derived measles virus vaccine.
abstract: Despite eradication attempts, measles remains a global health concern. Here we report results that demonstrate that a single-dose DNA immunization followed by multiple boosters, delivered orally as a plant-derived vaccine, can induce significantly greater quantities of measles virus-neutralizing antibodies than immunization with either DNA or plant-derived vaccines alone. This represents the first demonstration of an enhanced immune response to a prime-boost vaccination strategy combining a DNA vaccine with edible plant technology.
authors.affiliation: Department of Medicine, Monash University Medical School, Alfred Hospital, Prahran, Victoria 3181, Australia.
authors.forename: Diane E
authors.initials: DE
authors.lastname: Webster
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Cooney
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Huang
authors.forename: Damien R
authors.initials: DR
authors.lastname: Drew
authors.forename: Ian A
authors.initials: IA
authors.lastname: Ramshaw
authors.forename: Ian B
authors.initials: IB
authors.lastname: Dry
authors.forename: Richard A
authors.initials: RA
authors.lastname: Strugnell
authors.forename: Jenny L
authors.initials: JL
authors.lastname: Martin
authors.forename: Steve L
authors.initials: SL
authors.lastname: Wesselingh
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000276:immunology*; D007117:Immunization, Secondary; D008457:Measles / Q000517:prevention & control*; D008458:Measles Vaccine / Q000276:immunology*; D008459:Measles virus / Q000276:immunology; D051379:Mice; D009500:Neutralization Tests; D030821:Plants, Genetically Modified / Q000276:immunology*; D014026:Nicotiana / Q000235:genetics; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D008458:Measles Vaccine; D019444:Vaccines, DNA; C032397:hemagglutinin protein G, measles virus