Construction and intergeneric conjugative transfer of a pSG5-based cosmid vector from Escherichia coli to the polyisoprene rubber degrading strain Micromonospora aurantiaca W2b.
A non-rubber degrading mutant of the polyisoprene rubber degrading bacterium Micromonospora aurantiaca W2b lacking the capability to form halos on latex overlay agar plates was isolated after N-methyl-N-nitro-N-nitrosoguanidine mutagenesis. A 10.3-kb shuttle cosmid vector pGM446 was constructed from the Streptomyces cloning vectors pGM160 and pOJ446. This vector was transferred by conjugation from Escherichia coli to M. aurantiaca W2b. The frequency of formation of exconjugants with pGM446 was 3.6 x 10(-3). This vector could be useful for shotgun cloning of genes into the non-rubber degrading mutant L1 from M. aurantiaca W2b.
Rose
Karsten
K
Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, D-48149, Münster, Germany.
Steinbüchel
Alexander
A
D002070:Butadienes / Q000378:metabolism; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D045782:Hemiterpenes; D006358:Hot Temperature; D008847:Micromonospora / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008957:Models, Genetic; D009154:Mutation; D010420:Pentanes; D010957:Plasmids; D012408:Rubber / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics
D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene; D012408:Rubber