Molecular chaperones and the process of cellular immortalization in vitro.
It is suggested that the chaperone-activity of the SV40 T protein is responsible for the first step in the process of cellular immortalization in vitro: the extension of life span of most transfected cells. Further that an additional up-regulation of selected molecular chaperones is causal in the second step of the process: the rare acquisition of an unlimited cell division potential. Possibly the molecular chaperones are evolution facilitators, enabling the immortalization of primary cells in vitro, as well as the evolution of longevity in species.
Krøll
J
J
Department of Clinical Chemistry, Blekinge County Hospital, Karlskrona, Sweden; Present address: Godthåbsvej 111, 3th, DK-2000, Frederiksberg, Denmark. krollj@danbbs.dk
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology; D006801:Humans; D008565:Membrane Proteins / Q000502:physiology; D018832:Molecular Chaperones / Q000502:physiology*; D019098:Telomerase / Q000502:physiology
D000952:Antigens, Polyomavirus Transforming; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; C018551:glucose-regulated proteins; D019098:Telomerase