title: Software and methods for oligonucleotide and cDNA array data analysis.
abstract: Two HTML-based programs were developed to analyze and filter gene-expression data: 'Bullfrog' for Affymetrix oligonucleotide arrays and 'Spot' for custom cDNA arrays. The programs provide intuitive data-filtering tools through an easy-to-use interface. A background subtraction and normalization program for cDNA arrays was also built that provides an informative summary report with data-quality assessments. These programs are freeware to aid in the analysis of gene-expression results and facilitate the search for genes responsible for interesting biological processes and phenotypes.
authors.affiliation: The Salk Institute for Biological Studies, Laboratory of Genetics, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA. dlockhart@ambitbio.com
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Zapala
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Lockhart
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Pankratz
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Garcia
authors.forename: Carrolee
authors.initials: C
authors.lastname: Barlow
authors.forename: David J
authors.initials: DJ
authors.lastname: Lockhart
mesh_terms: D019295:Computational Biology / Q000295:instrumentation / Q000379:methods; D018076:DNA, Complementary / Q000032:analysis* / Q000235:genetics; D003628:Database Management Systems / Q000639:trends; D030541:Databases, Genetic / Q000639:trends; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D009841:Oligonucleotides / Q000235:genetics*; D012984:Software / Q000639:trends
chemical_list: D018076:DNA, Complementary; D009841:Oligonucleotides