Caspr3 and caspr4, two novel members of the caspr family are expressed in the nervous system and interact with PDZ domains.
The NCP family of cell-recognition molecules represents a distinct subgroup of the neurexins that includes Caspr and Caspr2, as well as Drosophila Neurexin-IV and axotactin. Here, we report the identification of Caspr3 and Caspr4, two new NCPs expressed in nervous system. Caspr3 was detected along axons in the corpus callosum, spinal cord, basket cells in the cerebellum and in peripheral nerves, as well as in oligodendrocytes. In contrast, expression of Caspr4 was more restricted to specific neuronal subpopulations in the olfactory bulb, hippocampus, deep cerebellar nuclei, and the substantia nigra. Similar to the neurexins, the cytoplasmic tails of Caspr3 and Caspr4 interacted differentially with PDZ domain-containing proteins of the CASK/Lin2-Veli/Lin7-Mint1/Lin10 complex. The structural organization and distinct cellular distribution of Caspr3 and Caspr4 suggest a potential role of these proteins in cell recognition within the nervous system.
Spiegel
Ivo
I
Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel.
Salomon
Daniela
D
Erne
Beat
B
Schaeren-Wiemers
Nicole
N
Peles
Elior
E
D000328:Adult; D000368:Aged; D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis; D029721:Drosophila Proteins; D006801:Humans; D007150:Immunohistochemistry; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D008875:Middle Aged; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000302:isolation & purification*; D009420:Nervous System / Q000166:cytology / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
C491323:CNTNAP1 protein, human; C464567:CNTNAP3 protein, human; D015816:Cell Adhesion Molecules, Neuronal; C491322:Cntnap1 protein, mouse; C105177:Cntnap1 protein, rat; C464568:Cntnap4 protein, mouse; D018076:DNA, Complementary; D029721:Drosophila Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C491321:Nrx protein, Drosophila; D012333:RNA, Messenger; D011956:Receptors, Cell Surface