Glucidic determinants expressed by the digestive apparatus of Umbrina cirrosa (L.) fries as revealed by lectin histochemistry.
Glycoconjugates present in the various parts of the digestive apparatus (oesophagus, stomach, intestine) of 27, 34 and 44 days-old Umbrina cirrosa (L.) fries were characterised by means of lectin histochemistry in conjunction with sialidase digestion and KOH treatment. High amounts of carbohydrates were detected especially in goblet cells of the oesophagus. Positivity obtained with the various lectins in oesophagus, stomach and intestine did not change during the development of the fries examined. D-Gal and D-GalNAc appeared to occupy a terminal position in oligosaccharidic chains whereas they are linked to sialic acid residues in adult subjects, which suggests an incomplete chemical structure of the glycoconjugates secreted by the digestive apparatus of fries.
Parillo
Francesco
F
Department of Biopathological Veterinary Science, Faculty of Veterinary Medicine, Università degli Studi di Perugia, Italy. anatvet@unipg.it
Fagioli
Oliviero
O
Ceccarelli
Piero
P
D000818:Animals; D004396:Coloring Agents; D004064:Digestive System / Q000378:metabolism*; D004947:Esophagus / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D006027:Glycosides / Q000096:biosynthesis*; D006651:Histocytochemistry; D006878:Hydroxides; D007413:Intestinal Mucosa / Q000378:metabolism; D037102:Lectins; D009439:Neuraminidase / Q000737:chemistry; D009844:Oligosaccharides / Q000378:metabolism; D010473:Perciformes / Q000502:physiology*; D010544:Peroxidases; D017680:Potassium Compounds; D013194:Staining and Labeling
D004396:Coloring Agents; D006027:Glycosides; D006878:Hydroxides; D037102:Lectins; D009844:Oligosaccharides; D017680:Potassium Compounds; D010544:Peroxidases; D009439:Neuraminidase; C029943:potassium hydroxide