title: Genetic vaccines protect against Sin Nombre hantavirus challenge in the deer mouse (Peromyscus maniculatus).
abstract: We used a deer mouse (Peromyscus maniculatus) infection model to test the protective efficacy of genetic vaccine candidates for Sin Nombre (SN) virus that were known to provoke immunological responses in BALB/c mice (Bharadwaj et al., Vaccine 17, 2836-2843, 1999 ). Protective epitopes were localized in each of four overlapping cDNA fragments that encoded portions of the SN virus G1 glycoprotein antigen; the nucleocapsid gene also was protective. The protective efficacy of glycoprotein gene fragments correlated with splenocyte proliferation in the presence of cognate antigen, but none induced neutralizing antibodies. Genetic vaccines against SN virus can protect outbred deer mice from infection even in the absence of a neutralizing antibody response.
authors.affiliation: Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
authors.forename: Mausumi
authors.initials: M
authors.lastname: Bharadwaj
authors.affiliation: Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
authors.forename: Katy
authors.initials: K
authors.lastname: Mirowsky
authors.affiliation: Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Ye
authors.affiliation: Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Botten
authors.affiliation: Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
authors.forename: Barbara
authors.initials: B
authors.lastname: Masten
authors.affiliation: Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
authors.forename: Joyce
authors.initials: J
authors.lastname: Yee
authors.affiliation: Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
authors.forename: C Richard
authors.initials: CR
authors.lastname: Lyons
authors.affiliation: Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Hjelle
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D018076:DNA, Complementary; D018778:Hantavirus Infections / Q000276:immunology / Q000517:prevention & control*; D007273:Injections, Intramuscular; D051379:Mice; D019251:Nucleocapsid / Q000235:genetics / Q000276:immunology; D010542:Peromyscus; D029261:Sin Nombre virus / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage*
chemical_list: D000914:Antibodies, Viral; D018076:DNA, Complementary; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C104008:glycoprotein G1, Hantavirus