title: Phenylethylamine induces an increase in cytosolic Ca2+ in yeast.
abstract: Beta-phenylethylamine (PEA) induced an increase in cytosolic free calcium ion concentration ([Ca2+]c) in Saccharomyces cerevisiae cells monitored with transgenic aequorin, a Ca2+-dependent photoprotein. The PEA-induced [Ca2+]c increase was dependent on the concentrations of PEA applied, and the Ca2+ mostly originated from an extracellular source. Preceding the Ca2+ influx, H2O2 was generated in the cells by the addition of PEA. Externally added H2O2 also induced a [Ca2+]c increase. These results suggest that PEA induces the [Ca2+]c increase via H2O2 generation. The PEA-induced [Ca2+]c increase occurred in the mid1 mutant with a slightly smaller peak than in the wild-type strain, indicating that Mid1, a stretch-activated nonselective cation channel, may not be mainly involved in the PEA-induced Ca2+ influx. When PEA was applied, the MATa mid1 mutant was rescued from alpha-factor-induced death in a Ca2+-limited medium, suggesting that the PEA-induced [Ca2+]c increase can reinforce calcium signaling in the mating pheromone response pathway.
authors.affiliation: Nagoya University Bioscience Center, Nagoya University, Japan.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Pinontoan
authors.forename: Svetlana
authors.initials: S
authors.lastname: Krystofova
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kawano
authors.forename: Izumi C
authors.initials: IC
authors.lastname: Mori
authors.forename: Frederick I
authors.initials: FI
authors.lastname: Tsuji
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Iida
authors.forename: Shoshi
authors.initials: S
authors.lastname: Muto
mesh_terms: D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects* / Q000378:metabolism; D000072235:Mating Factor; D010455:Peptides / Q000378:metabolism; D010627:Phenethylamines / Q000494:pharmacology*; D010675:Pheromones / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism
chemical_list: D010455:Peptides; D010627:Phenethylamines; D010675:Pheromones; C029261:phenethylamine; D000072235:Mating Factor; D002118:Calcium