Inhibitory Effects of Antioxidants on NF-KappaB Activation in Bovine Carotid Artery Endothelial Cells.
NF-Kappa B is one of important transcription factor, which plays an important role in many immune and inflammatory responses. A large variety of stimulus can induce the degradation of IKappa B-alpha, an inhibitor of NF-KappaB, leading to the activation and translocation of NF-KappaB into nuclei. In the present study, the effects of antioxidants on TNF-alpha-induced NF-KappaB activation were tested by using cultured bovine carotid artery endothelial cells. The degradation of IKappaB-alpha were checked by Western blot, and the nuclear translocation of NF-KappaB were tested by immunofluorescence. Our results showed that pyrrolidine dithio-carbamate (PDTC) completely inhibited IKappaB-alpha degradation and nuclear translocation of NF-KappaB induced by TNF-alpha, and other antioxidants also exhibited inhibitory activities on TNF-alpha-induced I B-alpha degradation.
Zhao
Feng
F
Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110015, China. xsyao@ihw.com.cn
Murota
Sei-Itsu
SI
Morita
Ikuo
I
Yao
Xin-Sheng
XS