Study of the first hours of microvinification by the use of osmotic stress-response genes as probes.
When yeast cells are inoculated into grape must for vinification they find stress conditions because of osmolarity, which is due to very high sugar concentration, and pH lower than 4. In this work an analysis of the expression of three osmotic stress induced genes (GPD1, HSP12 and HSP104) under microvinification conditions is shown as a way to probe those stress situations and the regulatory mechanisms that control them. The results indicate that during the first hours of microvinification there is an increase in the GPDI mRNA levels with a maximum about one hour after inoculation, and a decrease in the amount of HSP12 and HSP104 mRNAs, although with differences between them. The RNA steady-state levels of all the genes considered, and in some cases the microvinification progress are significantly affected by the composition of the must (pH, nature of the osmotic agent and carbon source). These results point out the importance of the control of these parameters and the yeast molecular response during the first hours of vinification for an accurate winemaking process.
Pérez-Torrado
Roberto
R
Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de Valencia, Burjassot, Spain.
Carrasco
Purificación
P
Aranda
Agustín
A
Gimeno-Alcañiz
José
J
Pérez-Ortín
José E
JE
Matallana
Emilia
E
del Olmo
Marcel Lí
ML
D015415:Biomarkers / Q000032:analysis; D015342:DNA Probes; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009997:Osmotic Pressure; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013997:Time Factors
D015415:Biomarkers; D015342:DNA Probes; C112591:HSP12 protein, S cerevisiae; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C070972:HsP104 protein, S cerevisiae; D005993:Glycerolphosphate Dehydrogenase; D005947:Glucose