title: Synthesis of Ser-His-Gly.DNA conjugates.
abstract: There are strong interactions between the bases of oligonucleotides. Based on Watson-Crick principle, they can form stable secondary and tertiary structure such as hairpin, duplex, triplex, G-quartet, pseudoknot, which can serve as the scaffold of molecules. Peptides contain active groups such as amino, carboxyl, imidazole, hydroxyl. A protected Ser-His-Gly-Threoninol phosphoramidite was synthesized in this work and was incorporated into a triplex-forming oligonucleotide. The results indicate that the oligonucleotide containing Ser-His-Gly-Threoninol in the middle could still form triplex with the target duplex and the dissociation constant was 0.5 micromol/L.
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Xi-Qing
authors.initials: XQ
authors.lastname: Li
authors.forename: Hai
authors.initials: H
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Zhuang
authors.forename: Bo
authors.initials: B
authors.lastname: Tan
authors.forename: Yu-Fen
authors.initials: YF
authors.lastname: Zhao
authors.forename: Chang-De
authors.initials: CD
authors.lastname: Lu
mesh_terms: D004247:DNA / Q000737:chemistry*; D005998:Glycine / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D012694:Serine / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009841:Oligonucleotides; D009842:Oligopeptides; C051802:triplex DNA; D012694:Serine; D006639:Histidine; D004247:DNA; D005998:Glycine