title: Propionibacterium acnes-induced IL-8 production may be mediated by NF-kappaB activation in human monocytes.
abstract: Propionibacterium acnes (P. acnes) causes an inflammatory acne that is characterized by massive neutrophilic infiltration. IL-8 is thought to play an important role in the pathophysiology of P. acnes, although the mechanisms by which P. acnes up-regulates the release of IL-8, a neutrophilic chemokine, from target cells is not well understood. In this study, we investigated the mechanisms through which heat-killed P. acnes induces IL-8 production in THP-1 cells (a human monocytic cell line). We found that P. acnes is able to directly induce IL-8 production and IL-8 mRNA expression in human monocytic cells in a dose- and time-dependent manner through a mechanism requiring transcription factor NF-kappaB activation. Additionally, P. acnes-induced IL-8 secretion was inhibited by roxithromycin, a macrolide antibiotic, and its inhibitory effect seemed to be partially associated with the inhibition of P. acnes-induced NF-kappaB activation. This is the first study to show that NF-kappaB activation is involved in the IL-8 production of monocytic cells stimulated by P. acnes.
authors.affiliation: Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. cheng@dermatol.med.kyushu-u.ac.jp
authors.forename: QiJie
authors.initials: Q
authors.lastname: Chen
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Koga
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uchi
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Hara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Terao
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Moroi
authors.forename: Kazunori
authors.initials: K
authors.lastname: Urabe
authors.forename: Masutaka
authors.initials: M
authors.lastname: Furue
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002460:Cell Line; D006706:Homeostasis; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D009000:Monocytes / Q000378:metabolism* / Q000382:microbiology*; D016328:NF-kappa B / Q000187:drug effects / Q000502:physiology*; D011425:Propionibacterium acnes / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D015575:Roxithromycin / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D000900:Anti-Bacterial Agents; D016209:Interleukin-8; D016328:NF-kappa B; D012333:RNA, Messenger; D015575:Roxithromycin