title: Analysis of herpes simplex virus entering into cells of oral origin.
abstract: The entry of herpes simplex virus (HSV) into an oral epithelial cell line, primary normal human oral keratinocytes (NHOK) and gingival fibroblasts (GF) was examined. Infection of these cells by HSV-1 and HSV-2 was blocked by heparin. Further examination indicated that heparin reduced viral attachment but not penetration. Moreover, neomycin inhibited HSV-1 infection more effectively than HSV-2 infection in GF, but not in NHOK. In conclusion, our results elucidated some aspects of the HSV entry process into oral cells and revealed some differences in HSV entering into NHOK and GF.
authors.affiliation: Institute of Oral Biology, National Yang-Ming University, No. 155, Sec. 2, Li-Nong Street, Pei-Tou, Taipei, Taiwan. slhung@ym.edu.tw
authors.forename: Shan Ling
authors.initials: SL
authors.lastname: Hung
authors.forename: Yi Hui
authors.initials: YH
authors.lastname: Wang
authors.forename: Hsiao Wang
authors.initials: HW
authors.lastname: Chen
authors.forename: Pei Lun
authors.initials: PL
authors.lastname: Lee
authors.forename: Yen Ting
authors.initials: YT
authors.lastname: Chen
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D005347:Fibroblasts / Q000821:virology*; D006801:Humans; D015603:Keratinocytes / Q000821:virology*; D009061:Mouth Mucosa / Q000821:virology; D018139:Simplexvirus / Q000502:physiology*; D014709:Vero Cells; D010948:Viral Plaque Assay