Measurement of genome wide DNA methylation by reversed-phase high-performance liquid chromatography.
A method to determine the extent of cytosine methylation in DNA is described. The technique involves the enzymatic hydrolysis of DNA to its deoxyribonucleotide components and subsequent separation and quantification of the nucleotides by isocratic reversed-phase high-performance liquid chromatography (HPLC). The system gives highly reproducible results and, under suitable conditions, is capable of measuring 5-methylcytosine levels in as little as 1 microg of DNA.
Ramsahoye
B H
BH
John Hughes Bennett Laboratory, Department of Haematology, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.
D002138:Calibration; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003562:Cytidine / Q000031:analogs & derivatives* / Q000378:metabolism; D003568:Cytidine Monophosphate / Q000032:analysis / Q000378:metabolism; D019175:DNA Methylation; D006801:Humans
D003562:Cytidine; D003568:Cytidine Monophosphate; C016568:5-methylcytidine