Activated microglia (BV-2) facilitation of TNF-alpha-mediated motor neuron death in vitro.
We have studied the interactions between activated microglia and injured motor neurons using an immortalized murine microglial cell line (BV-2) stimulated with either lipopolysaccharide (LPS) (Escherichia coli) or supernatant from serum-deprived motor neurons (NSC-34 cell line). Both stimuli induced BV-2 activation. Although both BV-2 supernatants induced a subsequent increase in NO generation in otherwise healthy NSC-34 cells, only LPS-activated microglial supernatant induced NSC-34 cell death through a TNF-alpha-dependent pathway. However, we observed a 20-fold increase in the amount of TNF-alpha required to kill NSC-34 cells in the absence of LPS-activated BV-2 cell supernatant, indicating that microglia secrete factor(s) that facilitate TNF-alpha-mediated motor neuron death in vitro.
He
Bei Ping
BP
Neurodegeneration Research Group, The John P. Robarts Research Institute, University of Western Ontario, London, Ontario, Canada N6A 5A5. mstrong@uwo.co
Wen
Weiyan
W
Strong
Michael J
MJ
D000690:Amyotrophic Lateral Sclerosis / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002450:Cell Communication / Q000276:immunology*; D016923:Cell Death / Q000276:immunology*; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D002490:Central Nervous System / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D005911:Gliosis / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000276:immunology* / Q000378:metabolism; D009046:Motor Neurons / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology
D000906:Antibodies; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase