An enzymatic fluorometric assay for pyridoxal with high specificity and sensitivity.
An enzymatic fluorometric assay for pyridoxal with pyridoxal dehydrogenase was developed. The detection limit was about 10 pmol: the calibration curve of pyridoxal showed high linearity (r=0.993). The values obtained by this method correlated well with those by the HPLC method. The enzyme had a high specificity for pyridoxal, and thus animal samples could be directly analyzed without separation of pyridoxal 5'-phosphate by column chromatography.
Trongpanich
Yanee
Y
Department of Bioresources Science, Faculty of Agriculture, Kochi University, Japan.
Mito
Mitsunori
M
Yagi
Toshiharu
T
D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D011730:Pyridoxal / Q000032:analysis*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D011730:Pyridoxal; D000429:Alcohol Oxidoreductases; C042642:pyridoxal dehydrogenase