title: [Experimental study and clinical application on osteogenesis of percutaneous autogenous bone marrow grafting in bone defects].
abstract: OBJECTIVE
  To observe the osteogenesis of percutaneous autogenous bone marrow grafting in cicatricial bone defect, to seek a good method for treating fracture nonunion.
  
  
  METHODS
  Eighteen rabbits were adopted in this study. 1 cm bone defect model was made in each side of radius, 6 weeks later, 2 ml autogenous bone marrow was injected in the right radial bone defect as experimental group, 2 ml autogenous peripheral blood in the left side as control group. X-ray features, histologic changes, Ca and P content in the site of bone defect were studied in various times. Also 15 patients were treated clinically for the nonunion fracture, the average time from nonunion to bone marrow grafting was 13 months.
  
  
  RESULTS
  In experimental group, the increasing new bone tissue were observed in X-ray and histologic examination. While in control group, no osteogenesis was observed. Ca and P content of experimental group was higher than that of control group. For the 15 patients, 13 cases healed in 5-9 months, 2 cases failed.
  
  
  CONCLUSIONS
  Percutaneous autogenous bone marrow grafting is capable of osteogenesis in the cicatricial bone defects. It can be used in nonunion cases which are not fit for operation of bone grafting because of poor condition of the skin.
authors.affiliation: Department of Orthopedic Surgery, General Hospital of PLA, Beijing, P. R. China 100853.
authors.forename: Y T
authors.initials: YT
authors.lastname: Liang
authors.forename: B X
authors.initials: BX
authors.lastname: Zhang
authors.forename: S B
authors.initials: SB
authors.lastname: Lu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D016026:Bone Marrow Transplantation; D002921:Cicatrix; D005260:Female; D017102:Fracture Healing; D005599:Fractures, Ununited / Q000628:therapy*; D006801:Humans; D006810:Humeral Fractures / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D010012:Osteogenesis; D011817:Rabbits; D011885:Radius Fractures / Q000628:therapy*; D013978:Tibial Fractures / Q000628:therapy