Characterization of an HIV type 1 strain with preferential replication in adherent cells.
HIV-E, emerging from persistently infected HeLa-T4 cells, replicates better in fibroblasts and epithelial cells with respect to the parental, T cell-derived HIV-T. The two viruses share the same env V3 loop, but differ in cellular molecules incorporated on the envelope. Even when similar amounts of virus attachment occurred, HIV-E replicated better than HIV-T in cells from solid tissues, and the response to exogenous Tat was more efficient. This might be related to the long terminal repeat (LTR), because HIV-E has a TAR duplication, and a mutation in the Sp1-II binding site. Epithelial cells deserve further study, because they may be important in vivo for variant selection and latency.
Serra
Caterina
C
Section of Clinical and Experimental Microbiology, Department of Biomedical Sciences, University of Sassari, I-07100 Sassari, Italy.
Mameli
Giuseppe
G
Biolchini
Adriana
A
Ziccheddu
Maria
M
Curreli
Sabrina
S
Arru
Giannina
G
Dolei
Antonina
A
D001483:Base Sequence; D004847:Epithelial Cells / Q000821:virology*; D005347:Fibroblasts / Q000821:virology*; D015696:Gene Products, tat / Q000502:physiology; D016325:HIV Long Terminal Repeat / Q000502:physiology; D015497:HIV-1 / Q000145:classification / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D012367:RNA, Viral / Q000032:analysis; D014779:Virus Replication / Q000502:physiology*; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D012367:RNA, Viral; D054322:tat Gene Products, Human Immunodeficiency Virus