Expression of Human Epiregulin in E.coli.
Human epiregulin cDNA was amplified from the lung cancer cell line A549 using RT-PCR. After adding 6 His codon to its 3' end, it was cloned into a high efficient secretive Escherichia coli system with alkaline phosphatase promoter(phoA promoter)constructed in our lab and induced for expression. The product was purified one-step by Ni-NTA column. Amino acid sequence analysis revealed the identity of our product with that previously reported. The product showed strong proliferative effect on fibroblast cell line Balb/c3T3 and growth inhibitory effect on epithelial carcinoma cell line A431.
Xi
Quan-Sheng
QS
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. zpli@sunm.shcnc.ac.cn
Qian
Xin-Guo
XG
Zhou
Qing-Wei
QW
Pan
Wei
W
Li
Zai-Ping
ZP
Gan
Ren-Bao
RB