title: Enzymatic hydrolysis of bacterial poly(3-hydroxybutyrate-co-3-hydroxypropionate)s by poly(3-hydroxyalkanoate) depolymerase from Acidovorax Sp. TP4.
abstract: Enzymatic degradability has been investigated for a series of bacterial poly(3-hydroxybutyrate-co-3-hydroxypropionate)s (P(3HB-co-3HP)s) with 3-hydroxypropionate (3HP) unit contents from 11 to 86 mol % as well as poly(3-hydroxybutyrate) (P(3HB)) and chemosynthesized poly(3-hydroxypropionate) (P(3HP)). The behavior of degradation by two types of extracellular poly(3-hydroxyalkanoate) (PHA) depolymerases purified from Ralstonia pikettii T1 and Acidovorax Sp. TP4, defined respectively as PHA depolymerase types I and II according to the position of the lipase box in the catalytic domain, were compared in relation to the thermal properties and crystalline structures of the PHA samples elucidated by differential scanning calorimetry and wide-angle X-ray diffraction. The degradation products were characterized by high-performance liquid chromatography and one- (1D) and two-dimension (2D) (1)H NMR spectroscopy. It was found that the PHA depolymerase of Acidovorax Sp. TP4 showed degradation behavior different from that shown by depolymerase of R. pikettii T1. PHA depolymerase from Acidovorax Sp. TP4 degraded the P(3HB-co-3HP) films with lower crystallinity in higher rates than those with higher crystallinity, no matter what kinds of crystalline structures they formed. In contrast, PHA depolymerase from R. pikettii T1 degraded P(3HB-co-3HP) films forming P(3HB) crystalline structure in higher rates than those forming P(3HP)s. The increase in amorphous nature of the P(3HB-co-3HP) films with P(3HB)-homopolymer-like crystalline structure increases and then decreases the rate of degradation by depolymerase from R. pikettii T1. The 3-hydroxybutyrate (3HB) monomer was produced as a major product by the hydrolysis of P(3HB) film by PHA depolymerase from Acidovorax Sp. TP4. The P(3HB-co-3HP) films could be degraded into 3HB and 3-hydroxypropionate (3HP) monomer at last, indicating that the catalytic domain of the enzyme recognized at least two monomeric units as substrates. While the PHA depolymerase from R. pikettii T1 hydrolyzed P(3HB) film into 3HB dimer as a major product, and the catalytic domain recognized at least three monomeric units. The degradation behavior of P(3HB-co-3HP) films by the PHA depolymerase of Acidovorax Sp. TP4 could be distinguished from that by the depolymerase of R. pikettii T1.
authors.affiliation: Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Inagawa
authors.forename: Terumi
authors.initials: T
authors.lastname: Saito
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Kasuya
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Doi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Inoue
mesh_terms: D020562:Betaproteobacteria / Q000201:enzymology; D001673:Biodegradation, Environmental; D002152:Calorimetry, Differential Scanning; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D016946:Gram-Negative Aerobic Rods and Cocci / Q000201:enzymology; D006868:Hydrolysis; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D014961:X-Ray Diffraction; D000094122:Polyhydroxybutyrates
chemical_list: D002265:Carboxylic Ester Hydrolases; D006885:Hydroxybutyrates; D007527:Isoenzymes; D011091:Polyesters; C432743:poly(3-hydroxybutyrate-co-3-hydroxypropionate); D000094122:Polyhydroxybutyrates; C022417:poly-beta-hydroxybutyrate depolymerase