Contribution of IL-18 to Th1 response and host defense against infection by Mycobacterium tuberculosis: a comparative study with IL-12p40.
The present study was conducted to critically determine the protective role of IL-18 in host response to Mycobacterium tuberculosis infection. IL-18-deficient (knockout (KO)) mice were slightly more prone to this infection than wild-type (WT) mice. Sensitivity of IL-12p40KO mice was lower than that of IL-12p40/IL-18 double KO mice. IFN-gamma production caused by the infection was significantly attenuated in IL-18KO mice compared with WT mice, as indicated by reduction in the levels of this cytokine in sera, spleen, lung, and liver, and its synthesis by spleen cells restimulated with purified protein derivatives. Serum IL-12p40 level postinfection and its production by peritoneal exudate cells stimulated with live bacilli were also significantly lower in IL-18KO mice than WT mice, suggesting that attenuated production of IFN-gamma was secondary to reduction of IL-12 synthesis. However, this was not likely the case, because administration of excess IL-12 did not restore the reduced IFN-gamma production in IL-18KO mice. In further studies, IL-18 transgenic mice were more resistant to the infection than control littermate mice, and serum IFN-gamma level and its production by restimulated spleen cells were increased in the former mice. Taken together, our results indicate that IL-18 plays an important role in Th1 response and host defense against M. tuberculosis infection although the contribution was not as profound as that of IL-12p40.
Kinjo
Yuki
Y
Faculty of Medicine, First Department of Internal Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan.
Kawakami
Kazuyoshi
K
Uezu
Kaori
K
Yara
Satomi
S
Miyagi
Kazuya
K
Koguchi
Yoshinobu
Y
Hoshino
Tomoaki
T
Okamoto
Masaki
M
Kawase
Yusuke
Y
Yokota
Koichi
K
Yoshino
Kohichiro
K
Takeda
Kiyoshi
K
Akira
Shizuo
S
Saito
Atsushi
A
D000818:Animals; D002478:Cells, Cultured; D020022:Genetic Predisposition to Disease; D007113:Immunity, Innate / Q000235:genetics; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D018664:Interleukin-12 / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D053762:Interleukin-12 Subunit p40; D020382:Interleukin-18 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D021122:Protein Subunits; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D014376:Tuberculosis / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control*
D053762:Interleukin-12 Subunit p40; D020382:Interleukin-18; D021122:Protein Subunits; D018664:Interleukin-12; D007371:Interferon-gamma