Activity of Sinorhizobium meliloti NodAB and NodH enzymes on thiochitooligosaccharides.
Rhizobium bacteria synthesize signal molecules called Nod factors that elicit responses in the legume root during nodulation. Nod factors, modified N-acylated beta-(1,4)-N-acetylglucosamine, are synthesized by the nodulation (nod) gene products. We tested the ability of three Sinorhizobium meliloti nod gene products to modify Nod factor analogs with thio linkages instead of O-glycosidic bonds in the oligosaccharide backbone.
Southwick
Audrey M
AM
Department of Biological Sciences, Stanford University, California 94305-5020, USA.
Wang
Lai-Xi
LX
Long
Sharon R
SR
Lee
Yuan C
YC
D000217:Acyltransferases / Q000378:metabolism*; D000581:Amidohydrolases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D016962:Sinorhizobium meliloti / Q000201:enzymology* / Q000254:growth & development; D015238:Sulfotransferases / Q000378:metabolism*; D014312:Trisaccharides / Q000378:metabolism*
D001426:Bacterial Proteins; D009844:Oligosaccharides; D014312:Trisaccharides; C073606:nodB protein, Bacteria; C041161:chitotriose; C012238:chitotetrose; D000217:Acyltransferases; C060770:NodA protein, Rhizobiales; C092976:NodH protein, Sinorhizobium meliloti; D015238:Sulfotransferases; D000581:Amidohydrolases