title: Mechanisms of metastasis suppression by introduction of human chromosome 10 into rat prostate cancer.
abstract: OBJECTIVE
  The metastatic ability of a Dunning R-3327 rat prostate cancer subline (AT6.3) was suppressed by the introduction of human chromosome 10, when these hybrid cancer cells were injected subcutaneously into nude mice (Nihei et al., Genes Chromosomes Cancer 14:112-119, 1995). The present study was undertaken to clarify which step of metastasis was suppressed in the human chromosome 10-containing microcell hybrids (AT 6.3-10 clones).
  
  
  METHODS
  Gelatin zymography, an in vitro invasion assay using a Boyden chamber and an intravenous metastasis assay involving the injection of hybrid cells into nude mice were performed.
  
  
  RESULTS
  Gelatin zymography revealed that AT6.3-10 microcell hybrid clones expressed the 72 kD type IV collagenase (MMP-2) at an almost equal level as control microcell hybrid clones. Both the invasiveness as measured by the invasion assay and the number of lung metastases as measured by the intravenous metastasis assay of AT6.3-10 hybrid clones were significantly less than those of the AT6.3 parental clone.
  
  
  CONCLUSIONS
  The human chromosome 10 suppresses both the local invasion and the metastatic process after entry into the blood circulation of rat prostate cancer. This decrease in local-invasive ability does not seem to require a decrease in MMP-2 activity.
authors.affiliation: Department of Molecular Oncology (M7), Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Hamano
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kuramochi
authors.forename: Naoki
authors.initials: N
authors.lastname: Nihei
authors.forename: Naoto
authors.initials: N
authors.lastname: Kamiya
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Igarashi
authors.forename: J Carl
authors.initials: JC
authors.lastname: Barrett
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Ichikawa
authors.forename: Haruo
authors.initials: H
authors.lastname: Ito
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002455:Cell Division; D002879:Chromosomes, Human, Pair 10; D005780:Gelatin / Q000032:analysis; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D051381:Rats; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology / Q000556:secondary; D014407:Tumor Cells, Cultured
chemical_list: D005780:Gelatin