title: Glial cell line-derived neurotrophic factor enhances survival of GM-CSF dependent rat GMIR1-microglial cells.
abstract: Microglial activation and proliferation occur in nearly all forms of brain injury. The aim of this study was to investigate the influence of glial cell-line derived neurotrophic factor (GDNF) on proliferation and/or survival in a GMIR1 rat microglial cell line, which proliferates in response to granulocyte-macrophage-colony stimulating factor (GM-CSF). Endogenous GDNF and its receptor, GFRalpha-1, were detected in GMIR1 cells by ELISA and immunohistochemistry/Western blot, respectively. Recombinant GDNF strongly enhanced GMIR1 cell numbers and BrdU-incorporation, an effect inhibited by GDNF blocking antibodies. Inhibition of cAMP/cGMP dependent protein kinase enhanced the GDNF-induced GMIR1 cell number. The results suggest that GDNF has synergistic survival promoting effects on microglia potentially via autocrine mechanisms.
authors.affiliation: Laboratory of Psychiatry, Department of Psychiatry, University of Innsbruck, Anichstrasse 35, Austria.
authors.forename: Kayvon
authors.initials: K
authors.lastname: Salimi
authors.forename: Karma
authors.initials: K
authors.lastname: Moser
authors.forename: Birgit
authors.initials: B
authors.lastname: Zassler
authors.forename: Markus
authors.initials: M
authors.lastname: Reindl
authors.forename: Norbert
authors.initials: N
authors.lastname: Embacher
authors.forename: Christine
authors.initials: C
authors.lastname: Schermer
authors.forename: Carla
authors.initials: C
authors.lastname: Weis
authors.forename: Josef
authors.initials: J
authors.lastname: Marksteiner
authors.forename: Makoto
authors.initials: M
authors.lastname: Sawada
authors.forename: Christian
authors.initials: C
authors.lastname: Humpel
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002452:Cell Count; D002461:Cell Line, Transformed / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D051100:Glial Cell Line-Derived Neurotrophic Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology*; D007150:Immunohistochemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D017628:Microglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000494:pharmacology / Q000502:physiology*; D009573:Nitrites / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013194:Staining and Labeling
chemical_list: C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009573:Nitrites; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D007770:L-Lactate Dehydrogenase