Evaluation of PCR-based beef sexing methods.
Analysis of the sex of beef meat by fast and reliable molecular methods is an important measure to ensure correct allocation of export refunds, which are considerably higher for male beef meat. Two PCR-based beef sexing methods have been optimized and evaluated. The amelogenin-type method revealed excellent accuracy and robustness, whereas the bovine satellite/Y-chromosome duplex PCR procedure showed more ambiguous results. In addition, an interlaboratory comparison was organized to evaluate currently applied PCR-based sexing methods in European customs laboratories. From a total of 375 samples sent out, only 1 false result was reported (female identified as male). However, differences in the performances of the applied methods became apparent. The collected data contribute to specify technical requirements for a common European beef sexing methodology based on PCR.
Zeleny
Reinhard
R
European Commission, Joint Research Center, Institute for Reference Materials and Measurements (IRMM), Retieseweg, 2440 Geel, Belgium. reinhard.zeleny@irmm.jrc.be
Bernreuther
Alexander
A
Schimmel
Heinz
H
Pauwels
Jean
J
D053523:Amelogenin; D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis; D003746:Dental Enamel Proteins; D019075:Electrophoresis, Capillary; D005260:Female; D008297:Male; D008460:Meat; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012732:Sex Determination Analysis; D014998:Y Chromosome
D053523:Amelogenin; D003746:Dental Enamel Proteins; D004247:DNA