Determination of remifentanil in human blood by capillary gas chromatography with nitrogen-selective detection.
A validated method for the determination of remifentanil in human blood, applicable to all therapeutic concentrations, using capillary GC with nitrogen-specific detection and fentanyl as the internal standard has been developed. Citrated whole blood samples were extracted into 1-chlorobutane following precipitation of proteins with methanol. The drugs were back extracted into 10 mM HCl and re-extracted into methanol-1-chlorobutane. The extracts were reconstituted in methanol and injected onto a 25-m BPX-5 column. The lower limit of quantitation was 0.2 ng/ml with within- and between-day coefficients of variation of less than 15%.
Bjorksten
A R
AR
Anaesthesia Research Laboratory, Department of Anaesthesia and Pain Management, The Royal Melbourne Hospital, Victoria 3050, Australia.
Chan
C
C
Crankshaw
D P
DP
D000701:Analgesics, Opioid / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D009584:Nitrogen; D010880:Piperidines / Q000097:blood*; D000077208:Remifentanil; D015203:Reproducibility of Results
D000701:Analgesics, Opioid; D010880:Piperidines; D009584:Nitrogen; D000077208:Remifentanil