title: Role of G protein beta3 subunit C825T and HLA class II polymorphisms in the immune response after HBV vaccination.
abstract: The G protein beta3 (GNB3) subunit and HLA are candidate genes predictive of immune response capacity. We therefore studied the influence of both gene systems on cellular and humoral immunity against hepatitis B virus (HBV) in 79 HBV booster-vaccinated healthy volunteers and an independent group of 77 probands after HBV basic immunization. Following booster vaccination, lymphocyte in vitro proliferation after stimulation with HBV surface antigen was 2.5-fold increased in GNB3 825T (TC + TT) vs CC allele carriers (P = 0.01) and was not influenced by HLA-DRB1 or DQB1 alleles. In addition, anti-HBs antibody titers in both groups were 2-fold increased in TC vs CC and decreased in TT vs CC allele carriers. However, antibody titers after HBV booster immunization were elevated in HLA-DQB1*0301 carriers (P corrected = 0.027). In summary, the GNB3 825T allele appears as a marker particularly predictive of cellular and HLA-DQB1*0301 of humoral immune responses following HBV vaccination.
authors.affiliation: Institut für Immunologie, Universitätsklinikum Essen, Essen, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Lindemann
authors.forename: Vahé
authors.initials: V
authors.lastname: Barsegian
authors.forename: Winfried
authors.initials: W
authors.lastname: Siffert
authors.forename: Stanislav
authors.initials: S
authors.lastname: Ferencik
authors.forename: Michael
authors.initials: M
authors.lastname: Roggendorf
authors.forename: Hans
authors.initials: H
authors.lastname: Grosse-Wilde
mesh_terms: D000328:Adult; D000483:Alleles; D005260:Female; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens / Q000235:genetics; D059866:HLA-DQ beta-Chains; D006509:Hepatitis B / Q000276:immunology* / Q000517:prevention & control; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics* / Q000502:physiology; D000949:Histocompatibility Antigens Class II / Q000502:physiology; D006801:Humans; D007117:Immunization, Secondary; D008213:Lymphocyte Activation; D008297:Male; D011110:Polymorphism, Genetic; D014611:Vaccination
chemical_list: C454420:G-protein beta3 subunit; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D000949:Histocompatibility Antigens Class II; D020962:Heterotrimeric GTP-Binding Proteins