title: Use of internal standard RNA molecules for the RT-PCR amplification of the faeces-borne RNA viruses.
abstract: The diagnostic system based on reverse transcription (RT)-PCR has been used widely for the detection of viral genomes of faecal-borne RNA viruses. However, faecal specimens often produce both false positive and false negative results. Therefore, there is a need for a diagnosis procedure that can control for 'false-results'. In this study, an internal standard RNA that can serve as a non-competitive positive template was developed and used directly to detect faecal-borne RNA viruses without noticeable competitive inhibition of the target viral genome. These results suggest that the internal standard RNA is a useful standard molecule when undertaking diagnostic qualitative RT-PCR procedures for enteroviruses and related faecal-borne RNA viruses.
authors.affiliation: Department of Life Science, College of Natural Science, Chung-Ang University, Heuksuk-dong 221, Dongjak-ku, Seoul 156-756, Republic of Korea.
authors.forename: Kisoon
authors.initials: K
authors.lastname: Kim
authors.forename: Jeongkoo
authors.initials: J
authors.lastname: Park
authors.forename: Yoonseok
authors.initials: Y
authors.lastname: Chung
authors.forename: Doosung
authors.initials: D
authors.lastname: Cheon
authors.forename: Il-Beom
authors.initials: IB
authors.lastname: Lee
authors.forename: Seokgyu
authors.initials: S
authors.lastname: Lee
authors.forename: Jaedeuk
authors.initials: J
authors.lastname: Yoon
authors.forename: Haewol
authors.initials: H
authors.lastname: Cho
authors.forename: Chulyong
authors.initials: C
authors.lastname: Song
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D005243:Feces / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D012327:RNA Virus Infections / Q000175:diagnosis*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral