title: Differential role of bFGF and VEGF for vasculogenesis.
abstract: Primary vascular plexus originate from angioblasts through a process called vasculogenesis. The precise role of basic fibroblast growth factor (bFGF) and the vascular endothelial growth factor (VEGF) are both suggested as key regulators in vasculogenesis is still unclear. This crucial aspect was investigated by using time lapse observation of in vitro generated embryonic stem (ES) cell-derived endothelial structures which were recognizable by using the platelet cell adhesion molecule (PECAM-1) driven endothelial-specific expression of the live reporter gene enhanced green fluorescent protein (EGFP). In serum free conditions VEGF led to improved survival of angioblasts and to the formation of primitive endothelial tubes whereas bFGF alone increased their survival. Our study suggests that the complex process of vasculogenesis can be driven by VEGF alone but not by bFGF.
authors.affiliation: Institute of Anatomy I and Institute of Neurophysiology, University of Cologne, Germany.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Kazemi
authors.forename: Daniela
authors.initials: D
authors.lastname: Wenzel
authors.forename: Eugen
authors.initials: E
authors.lastname: Kolossov
authors.forename: Nibedita
authors.initials: N
authors.lastname: Lenka
authors.forename: Axel
authors.initials: A
authors.lastname: Raible
authors.forename: Philipp
authors.initials: P
authors.lastname: Sasse
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hescheler
authors.forename: Klaus
authors.initials: K
authors.lastname: Addicks
authors.forename: Bernd K
authors.initials: BK
authors.lastname: Fleischmann
authors.forename: Wihelm
authors.initials: W
authors.lastname: Bloch
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D016228:Endothelial Growth Factors / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008222:Lymphokines / Q000494:pharmacology*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010957:Plasmids / Q000235:genetics; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2