Determination of nalidixic acid by fluorometry with sodium borohydride and hydrogen peroxide.
A simple and sensitive fluorometric method for the determination of nalidixic acid was established by using 0.75M sodium borohydride and 7.5% hydrogen peroxide solution as fluorogenic reagents. Analyte concentrations of 0.0232-11.6 pg/mL could be determined with high precision and accuracy by the method. A relative standard deviation of 1.75% was obtained for a nalidixic acid concentration of 0.232 pg/mL. The method was satisfactorily applied to the determination of nalidixic acid in human serum, fish muscle, and chicken muscle, and the calibration curves were linear from 0.23 to 58.00 pg/mL, from 9.28 to 32.48 mg/kg, and from 4.64 to 23.20 mg/kg, respectively. The specificity of the reaction is also discussed.
Wang
Mei-Lin
ML
Hung Kuang Institute of Technology, Sha Lu, Taichung, Taiwan, Republic of China. lyl123@ms9.hinet.net
Chen
Sheng-Chih
SC
Lien
Jin-Cherng
JC
Kuo
Sheng-Chu
SC
D000818:Animals; D001894:Borohydrides; D002645:Chickens; D004350:Drug Residues / Q000032:analysis; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D009132:Muscles / Q000737:chemistry; D009268:Nalidixic Acid / Q000032:analysis* / Q000097:blood; D012474:Salmon; D013696:Temperature
D001894:Borohydrides; D009268:Nalidixic Acid; D006861:Hydrogen Peroxide