[The effects of TGF-beta on the proliferation and differentiation of human osteoblast-like cells].
Osteoblast-like cells were isolated from embryonic human calvarium with modified enzyme digestion method. TGF-beta which is believed having biological effects on bone cells was added into the culture medium with separate concentrations. The incorporation of 3H-TdR, 3H-Proline of osteoblast-like cells and alkaline phosphatase(ALP) content of the cells were tested to study the effects of the TGF-beta on the proliferation of osteoblasts. The results showed that TGF-beta increased ALP activity and the synthesis of collagen, and the cell proliferation was inhibited. It affected the proliferation and differentiation of osteoblast-like cells with the best 1 ng/ml among four concentration of 0.01-10 ng/ml. These findings suggested that function of TGF-beta might be modified by other local growth factors.
Tian
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Department of Oral and Maxillofacial Surgery, West China University of Medical Sciences.
Wang
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D000469:Alkaline Phosphatase / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D005333:Fetus; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D012886:Skull / Q000166:cytology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D016212:Transforming Growth Factor beta; D003094:Collagen; D004247:DNA; D000469:Alkaline Phosphatase