title: Sequence-specific assignments of proton NMR resonance peaks and analysis of secondary structural elements of LC1, a novel antibacterial polypeptide.
abstract: LC1 is a type of novel antibacterial polypeptide secreted by a Bacillus subtilis strain. It consists of 47 residues. Using bioengineering, LC1 was expressed in E.coli DH5alpha by using recombinant plasmid PBVAB16. By means of two-dimensional DQF-COSY, TOCSY and NOESY spectroscopies, protons of all 47 residues are identified. The studies show that the secondary structures of LC1 are principally anti-parallel beta sheets and extended conformations. It was speculated that there may be a hydrophobic core around Trp(23) in its three-dimensional structure.
authors.affiliation: National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China. lugy@pku.edu.cn
authors.forename: Cheng-Hua
authors.initials: CH
authors.lastname: Shao
authors.forename: Jia-Peng
authors.initials: JP
authors.lastname: Zhu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cheng
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Wang
authors.forename: Wei-Bin
authors.initials: WB
authors.lastname: Gong
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.forename: Zhang-Liang
authors.initials: ZL
authors.lastname: Chen
authors.forename: Guang-Ying
authors.initials: GY
authors.lastname: Lu
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000737:chemistry; D001419:Bacteria / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology; D017433:Protein Structure, Secondary; D020539:Sequence Analysis, Protein / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides