The effect of nonsteroidal antiinflammatory drugs ibuprofen, flurbiprofen, and diclofenac on in vitro and in vivo growth of mouse fibrosarcoma.
For suppression of primary G:5:113 fibrosarcoma growth, three structurally different cyclooxygenase (COX) inhibitors (ibuprofen, flurbiprofen, and diclofenac) were administered intraperitoneally (i.p.) in two regimens starting on day 5 after tumor-cell inoculation. Repeated application of 0.15 mg/mouse/day during 14 consecutive days significantly suppressed the tumor growth and increased the percentage of surviving mice. Similar tendency, however without significant differences, was observed when animals were given 0.5 mg/day for five consecutive days. These results suggest that a time schedule of drug application is important for the therapeutic effect. Suppressive effect of diclofenac and flurbiprofen on tumor growth was also observed under in vitro conditions. We conclude that suppressive effect of these drugs on tumor growth in vivo comprises both direct effects of COX inhibitors on fibrosarcoma cells and indirect effects that are presumably mediated by extratumoral sources. Our findings encourage the use of COX inhibitors in the therapy of fibrosarcoma.
Hoferová
Zuzana
Z
Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic.
Fedorocko
Peter
P
Hofmanová
Jirina
J
Hofer
Michal
M
Znojil
Vladimír
V
Minksová
Katerina
K
Soucek
Karel
K
Egyed
Alena
A
Kozubík
Alois
A
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004008:Diclofenac / Q000627:therapeutic use*; D005354:Fibrosarcoma / Q000188:drug therapy* / Q000473:pathology; D005480:Flurbiprofen / Q000627:therapeutic use*; D007052:Ibuprofen / Q000627:therapeutic use*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009374:Neoplasms, Experimental; D015996:Survival Rate; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004008:Diclofenac; D005480:Flurbiprofen; D007052:Ibuprofen