[RP-HPLC determination of adenosine in fermented Cordyceps].
OBJECTIVE
A method for determination of adenosine in four fermented Cordyceps products by RP-HPLC is described.


METHODS
C18 column was used with methanol-0.06 mol/L potassium phosphate monobasic-tetrahydrofuran (10:150:1.5) as mobile phase. The flow rate was 1 ml/min and detected at 260 nm.


RESULTS
The average recovery was 98.7% and relative standard deviation was 1.10% (n = 5).


CONCLUSIONS
This method is simple, rapid and sensitive. The paper introduces the feasibility of supersonic extraction method taking the place of reflux method.
Li
X
X
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050.
Wang
Y
Y
Bao
T
T
D000241:Adenosine / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D032661:Cordyceps / Q000737:chemistry*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007915:Lepidoptera / Q000737:chemistry
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D000241:Adenosine