[Comparative study on osteogenesis of three types growth factors recombination artificial bones].
OBJECTIVE
To compare the osteogenesis of recombination artificial bones, which are bovine deproteined bone (bDPB) and bovine bone morphogenetic protein (bBMP), combined with tumor necrosis factor alpha (TNF alpha), basic fibroblast growth factor (bFGF), epidermal growth factor (EGF) respectively.


METHODS
One hundred trephined skull bone defects in fifty rabbits were divided into four groups, which implanted with bDPB/bBMP/TNF alpha, bDPB/bBMP/bFGF, bDPB/bBMP/EGF, and bDPB/bBMP respectively. X-ray and histological changes were observed in the 1st, 2nd, 4th, 6th, 8th weeks after implantation. The content of 35S and 45Ca and ash weight were measured at 10 and 42 days after operation.


RESULTS
The osteogenesis of bDPB/bBMP/TNF alpha group was stronger than that of bDPB/bBMP/bFGF group(P < 0.01), while bDPB/bBMP/bFGF group was stronger than that of bDPB/bBMP/EGF(P < 0.01). No significant statistical difference were found between bDPB/bBMP/EGF and bDPB/bBMP(P > 0.05).


CONCLUSIONS
TNF alpha combined with bBMP and carrier can stimulate bone formation and increase the volume of new bone in vivo. It suggests that bDPB/bBMP/TNF alpha is a valuable biomaterial of bone graft.
Ni
W D
WD
Department of Orthopedic Surgery, First Affiliated Hospital, Chongqing University of Medical Science, Chongqing, P. R. China 400016.
An
H
H
Jiang
D M
DM
D000818:Animals; D019485:Bone Morphogenetic Proteins; D016025:Bone Transplantation / Q000379:methods*; D004815:Epidermal Growth Factor; D016222:Fibroblast Growth Factor 2; D010012:Osteogenesis; D019736:Prostheses and Implants; D011817:Rabbits; D012887:Skull Fractures / Q000601:surgery; D014409:Tumor Necrosis Factor-alpha
D019485:Bone Morphogenetic Proteins; D014409:Tumor Necrosis Factor-alpha; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor