Defects in mandibular bone area, enamel iron content and dentine formation following gastrectomy in rats.
Fourteen 5-week-old male Sprague-Dawley rats were equally divided into two groups, sham-operated and gastrectomized. Tetracycline and calcein were given to label dentine. Four weeks after surgery, blood was collected for measurement of serum iron, calcium and parathyroid hormone (PTH) and the mandibles and maxillae were then removed. Sagittal sections of the maxilla or cross-sections of the mandible were prepared and examined. Backscattered electron images of the maxilla were taken and the iron content at the neck of incisors was measured by energy-dispersive X-ray. The dentine apposition rate in maxillary incisors was measured by fluorescence microscopy. Serum iron was significantly decreased, while PTH was significantly elevated without any change in the serum calcium in gastrectomized rats. Gastrectomy caused a gross loss of iron content in superficial enamel. The dentine apposition rate was significantly reduced by 30%. Both cortical and cancellous bone in the mandibula were significantly reduced. However, the total bone area in gastrectomized rats was similar to that in sham-operated rats. These results suggest that bone resorption was enhanced and dentine formation was reduced after gastrectomy.
Morohashi
Tomio
T
Department of Pharmacology, School of Dentistry, Showa University, Hatanodai 1-5-8, Shinagawa-ku, Tokyo 142-8555, Japan. morohasi@dent.showa-u.ac.jp
Hirama
Yuka
Y
Takahara
Sawa
S
Sano
Tsuneyoshi
T
Saitoh
Shigeru
S
Ohta
Atsutane
A
Sasa
Ryuji
R
Yamada
Shoji
S
D018798:Anemia, Iron-Deficiency / Q000503:physiopathology; D000818:Animals; D001862:Bone Resorption / Q000473:pathology; D002118:Calcium / Q000097:blood / Q000493:pharmacokinetics; D003743:Dental Enamel / Q000737:chemistry*; D003804:Dentin / Q000473:pathology; D003810:Dentinogenesis / Q000502:physiology*; D004577:Electron Probe Microanalysis; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005743:Gastrectomy / Q000009:adverse effects; D007180:Incisor / Q000737:chemistry; D007501:Iron / Q000032:analysis* / Q000097:blood; D008286:Malabsorption Syndromes / Q000503:physiopathology; D008297:Male; D008334:Mandible / Q000473:pathology*; D008437:Maxilla / Q000473:pathology; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010281:Parathyroid Hormone / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D013223:Statistics as Topic; D013752:Tetracycline
D005452:Fluoresceins; D005456:Fluorescent Dyes; D010281:Parathyroid Hormone; D007501:Iron; D013752:Tetracycline; D002118:Calcium; C007740:fluorexon