title: Fyn kinase initiates complementary signals required for IgE-dependent mast cell degranulation.
abstract: Fc epsilon RI activation of mast cells is thought to involve Lyn and Syk kinases proximal to the receptor and the signaling complex organized by the linker for activation of T cells (LAT). We report here that Fc epsilon RI also uses a Fyn kinase-dependent pathway that does not require Lyn kinase or the adapter LAT for its initiation, but is necessary for mast cell degranulation. Lyn-deficiency enhanced Fyn-dependent signals and degranulation, but inhibited the calcium response. Fyn-deficiency impaired degranulation, whereas Lyn-mediated signaling and calcium was normal. Thus, Fc epsilon RI-dependent mast cell degranulation involves cross-talk between Fyn and Lyn kinases.
authors.affiliation: Molecular Inflammation Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892-1820, USA.
authors.forename: Valentino
authors.initials: V
authors.lastname: Parravicini
authors.forename: Massimo
authors.initials: M
authors.lastname: Gadina
authors.forename: Martina
authors.initials: M
authors.lastname: Kovarova
authors.forename: Sandra
authors.initials: S
authors.lastname: Odom
authors.forename: Claudia
authors.initials: C
authors.lastname: Gonzalez-Espinosa
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Furumoto
authors.forename: Shinichiroh
authors.initials: S
authors.lastname: Saitoh
authors.forename: Lawrence E
authors.initials: LE
authors.lastname: Samelson
authors.forename: John J
authors.initials: JJ
authors.lastname: O'Shea
authors.forename: Juan
authors.initials: J
authors.lastname: Rivera
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism; D015550:Cell Degranulation / Q000276:immunology*; D003433:Crosses, Genetic; D004792:Enzyme Precursors / Q000276:immunology; D005260:Female; D015151:Immunoblotting; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000502:physiology*; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010750:Phosphoproteins / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000276:immunology; D011518:Proto-Oncogene Proteins / Q000172:deficiency / Q000276:immunology*; D051076:Proto-Oncogene Proteins c-fyn; D017455:Receptors, IgE / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology*; D000072377:Syk Kinase; D019061:src-Family Kinases / Q000276:immunology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D004792:Enzyme Precursors; C490897:Gab1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; C491734:Lat protein, mouse; D008565:Membrane Proteins; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D017455:Receptors, IgE; D011505:Protein-Tyrosine Kinases; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn; D000072377:Syk Kinase; C000606897:Syk protein, mouse; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases