title: Identification of three genes up-regulated in PU.1 rescued monocytic precursor cells.
abstract: The requirement of the transcription factor PU.1 for macrophage development has been well documented. However, the target genes regulated by PU.1 controlling macrophage maturation are not known. A granulocyte macrophage colony stimulating factor (GM-CSF)-dependent PU.1 null monocytic precursor cell was stably transduced with a PU.1-expressing retrovirus. The expression of PU.1 altered the surface expression of a few proteins expressed on monocytes; these cells, however, remained GM-CSF dependent and maintained an immature phenotype. In contrast to the PU.1 null cells, the cells expressing PU.1 responded to macrophage colony stimulating factor (M-CSF) with subsequent development into mature macrophages. Using suppressive subtractive hybridization between the PU.1 null and immature PU.1 rescued cells, three genes, MRP-14, Dap12 and CD53, were found expressed in the rescued cells, but not in the PU.1 null cells. In addition, these genes were modulated during M-CSF-induced maturation of the PU.1 rescued cells. The PU.1 null and rescued early monocytic cells provide a useful model to study the role of PU.1 in macrophage development.
authors.affiliation: The Burnham Institute, 10901 N. Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Henkel
authors.forename: Scott R
authors.initials: SR
authors.lastname: McKercher
authors.forename: Richard A
authors.initials: RA
authors.lastname: Maki
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D040502:Calgranulin B / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008565:Membrane Proteins; D009000:Monocytes / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D011971:Receptors, Immunologic / Q000235:genetics* / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics; D060265:Tetraspanin 25; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic; D015854:Up-Regulation; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D040502:Calgranulin B; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D012313:RNA; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D060265:Tetraspanin 25; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C554894:CD53 protein, human; C110546:TYROBP protein, human