title: Comparative study of the micellar enhanced spectrophotometric determination of beta-lactamic antibiotics by batch and flow injection analysis using a multisimplex design.
abstract: A study has been made on the spectrophotometric determination of the beta-lactamic antibiotics, amoxicillin and ampicillin, in micellar media using Cu ions as catalyst. Batch and flow injection approaches were compared. Multisimplex design was used to determine the optimal values of the flow injection analysis (FIA) system. Chemical (buffer, pH and Cu(II) concentrations) and physical (flow rate, temperature and reaction coil length) variables were considered. The analytical performance characteristics were as follows: the detection limits for batch and flow-through systems were 2.5 x 10(-7) and 2 x 10(-6) M, respectively, and a relative standard deviation less than 1% was found for both methods. The proposed FIA methodology was satisfactorily applied to the determination of the antibiotics in pharmaceutical formulations.
authors.affiliation: Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Fernández-González
authors.forename: R
authors.initials: R
authors.lastname: Badía
authors.forename: M E
authors.initials: ME
authors.lastname: Díaz-García
mesh_terms: D000658:Amoxicillin / Q000032:analysis*; D000667:Ampicillin / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002138:Calibration; D003300:Copper / Q000737:chemistry; D017022:Flow Injection Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D008823:Micelles; D013501:Surface-Active Agents; D003300:Copper; D000667:Ampicillin; D000658:Amoxicillin