title: Establishment of fluorescent lung carcinoma metastasis model and its real-time microscopic detection in SCID mice.
abstract: Lung cancer is the most prevalent malignant tumor in the world. Metastasis of the disease causes death in lung cancer patients. Recent study has shown that multiple cascades of gene defects occur in lung cancer. In this report, we established a novel H1299/EGFP tumor model to determine whether H1299 transfected with the enhanced green fluorescent protein (EGFP) gene in vitro and xenotransplanted into SCID mouse lung would permit the detection of lung cancer micrometastasis in vivo. We demonstrated that EGFP-transduced H1299 cells maintained stable high-level EGFP expressions during their growth in vivo. EGFP fluorescence clearly demarcated the primary seeding place and readily allowed for the visualization of distant micrometastasis and local invasion at the single-cell level. Small metastatic and locally invasive foci, including those immediately adjacent to the tumor's leading invasive edge, were almost undetectable by routine hematoxylin and eosin staining and immunohistochemistry. The GFP tagged lung cancer model is superior for the detection and study of physiologically relevant patterns of lung cancer invasion and metastasis in vivo.
authors.affiliation: Department of Internal Medicine, Kaohsiung Medical University, Taiwan.
authors.forename: Ming-Shyan
authors.initials: MS
authors.lastname: Huang
authors.forename: Tzu-Jou
authors.initials: TJ
authors.lastname: Wang
authors.forename: Chung-Ling
authors.initials: CL
authors.lastname: Liang
authors.forename: Huey-Mei
authors.initials: HM
authors.lastname: Huang
authors.forename: I-Chi
authors.initials: IC
authors.lastname: Yang
authors.forename: Hua
authors.initials: H
authors.lastname: Yi-Jan
authors.forename: Michael
authors.initials: M
authors.lastname: Hsiao
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology / Q000556:secondary*; D000328:Adult; D000818:Animals; D002277:Carcinoma / Q000737:chemistry*; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry / Q000473:pathology / Q000556:secondary*; D003199:Computer Systems; D005456:Fluorescent Dyes / Q000032:analysis*; D049452:Green Fluorescent Proteins; D006801:Humans; D007267:Injections; D008164:Luminescent Proteins / Q000032:analysis*; D008168:Lung; D008175:Lung Neoplasms / Q000737:chemistry / Q000473:pathology*; D051379:Mice; D016513:Mice, SCID; D008856:Microscopy, Fluorescence; D023421:Models, Animal; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000032:analysis*; D009368:Neoplasm Transplantation; D009928:Organ Specificity; D011993:Recombinant Fusion Proteins / Q000032:analysis; D014162:Transfection; D014183:Transplantation, Heterologous
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins