title: Crystal structure of a non-canonical high affinity peptide complexed with MHC class I: a novel use of alternative anchors.
abstract: The crystal structure of a non-standard peptide, YEA9, in complex with H-2Kb, at 1.5 A resolution demonstrates how YEA9 peptide can bind with surprisingly high affinity through insertion of alternative, long, non-canonical anchors into the B and E pockets. The use of "alternative pockets" represents a new mode of high affinity peptide binding, that should be considered when predicting peptide epitopes for MHC class I. These novel interactions encountered in this non-canonical high affinity peptide-MHC complex should help predict additional binding peptides from primary protein sequences and aid in the design of alternative approaches for peptide-based vaccines.
authors.affiliation: Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA. v.apostolopoulos@ari.unimelb.edu.au
authors.forename: Vasso
authors.initials: V
authors.lastname: Apostolopoulos
authors.forename: Minmin
authors.initials: M
authors.lastname: Yu
authors.forename: Adam L
authors.initials: AL
authors.lastname: Corper
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
authors.forename: Ian F C
authors.initials: IF
authors.lastname: McKenzie
authors.forename: Luc
authors.initials: L
authors.lastname: Teyton
authors.forename: Ian A
authors.initials: IA
authors.lastname: Wilson
authors.forename: Magdalena
authors.initials: M
authors.lastname: Plebanski
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D004330:Drosophila; D015195:Drug Design; D005656:Fungal Proteins / Q000737:chemistry / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000276:immunology; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D011485:Protein Binding; D011487:Protein Conformation; D014612:Vaccines
chemical_list: D005656:Fungal Proteins; D015395:Histocompatibility Antigens Class I; D010455:Peptides; D014612:Vaccines