title: The Pco proteins are involved in periplasmic copper handling in Escherichia coli.
abstract: The interactions between the plasmid-borne copper resistance determinant, pco, and the main copper export system in Escherichia coli have been investigated and no direct interaction has been found. The PcoE and PcoC proteins are periplasmic and PcoC binds one Cu ion per protein molecule. PcoA is also periplasmic and can substitute for the chromosomally encoded CueO protein. The pco determinant is proposed to exert its effect through periplasmic handling of excess copper ions and to increase the level of resistance to copper ions above that conferred by copA alone.
authors.affiliation: Department of Soil, Water, and Environmental Science, University of Arizona, Tucson 85721, USA.
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Lee
authors.forename: Gregor
authors.initials: G
authors.lastname: Grass
authors.forename: Christopher
authors.initials: C
authors.lastname: Rensing
authors.forename: Siobhán R
authors.initials: SR
authors.lastname: Barrett
authors.forename: Christopher J D
authors.initials: CJ
authors.lastname: Yates
authors.forename: Jivko V
authors.initials: JV
authors.lastname: Stoyanov
authors.forename: Nigel L
authors.initials: NL
authors.lastname: Brown
mesh_terms: D001665:Binding Sites; D001692:Biological Transport; D003300:Copper / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000502:physiology*; D007477:Ions; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D016296:Mutagenesis; D010088:Oxidoreductases / Q000502:physiology; D019897:Periplasm / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D007477:Ions; C462540:PcoA protein, E coli; C462541:PcoC protein, E coli; C462542:PcoE protein, E coli; D003300:Copper; D004247:DNA; D010088:Oxidoreductases; C499679:cueO protein, E coli