[Pathogenic protozoans in man: differential characterization of Entamoeba histolytica/Entamoeba dispar complex, Acanthamoeba spp., Microsporidia].
The review summarizes the results in the main parasitological topics of our Lab: amoebic infections due to Entamoeba histolytica/Entamoeba dispar complex and to Acanthamoeba spp. respectively, and human infections caused by microsporidia. Different rapid and advanced techniques have been included in the standardized diagnostic protocols for each topic, and a critical comparison among them was made, in order to define the gold standard diagnostic method: a) E. histolytica/E. dispar: in vitro culture, zymodeme typization, biomolecular identification (PCR), immunoenzymatic assay (ELISA) for direct detection in stools of specific surface antigenic lectins; b) Acanthamoeba spp.: in vitro culture, light and ultrastructural characterization, species identification by immunofluorescence method with monoclonal antibodies, in vitro pharmacological studies; c) Microsporidia: ultrastructural (TEM), biomolecular (PCR), biochemical and immunological (SDS-PAGE, Immunoblotting) studies for species identification, use of advanced ultrastructural techniques ("freeze-etching", "deep-etching") in order to deepen the spore wall structure, to study the cytoskeletal function of actin and to define the mode of infection, in vitro pharmacological assays on some inhibitors of chitin-synthases.
Scaglia
M
M
Dipartimento di Malattie Infettive, Laboratori di Ricerca di Area Infettivologica, Università-IRCCS Policlinico San Matteo, Viale Taramelli 5, 27100 Pavia. mscaglia@smatteo.pv.it
Gatti
S
S
Bruno
A
A
Bernuzzi
A M
AM
Cevini
C
C
Maserati
R
R
D000048:Acanthamoeba / Q000737:chemistry*; D000562:Amebiasis / Q000175:diagnosis*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004747:Entamoeba / Q000737:chemistry*; D004748:Entamoeba histolytica / Q000737:chemistry*; D004749:Entamoebiasis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016814:Microsporidia / Q000737:chemistry*; D016881:Microsporidiosis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction