Pendrin is an iodide-specific apical porter responsible for iodide efflux from thyroid cells.
The Pendred syndrome gene encodes a 780-amino acid putative transmembrane protein (pendrin) that is expressed in the apical membrane of thyroid follicular cells. Although pendrin was shown to transport iodide and chloride using Xenopus laevis oocytes and Sf9 insect cells, there is no report using mammalian cells to study its role in thyroid function. We show here, using COS-7 cells and Chinese hamster ovary cells transfected with expression vectors encoding sodium iodide symporter or human Pendred syndrome gene cDNA and by comparison with studies using rat thyroid FRTL-5 cells, that pendrin is an iodide-specific transporter in mammalian cells and is responsible for iodide efflux in the thyroid.
Yoshida
Akio
A
First Department of Internal Medicine, Tottori University Faculty of Medicine, Nishimachi 36-1, Yonago, Tottori 683-8504, Japan. ayoshida@bronze.ocn.ne.jp
Taniguchi
Shinichi
S
Hisatome
Ichiro
I
Royaux
Ines E
IE
Green
Eric D
ED
Kohn
Leonard D
LD
Suzuki
Koichi
K
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D016466:CHO Cells; D019556:COS Cells; D002352:Carrier Proteins / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D006224:Cricetinae; D006801:Humans; D007454:Iodides / Q000493:pharmacokinetics*; D007455:Iodine / Q000493:pharmacokinetics; D007457:Iodine Radioisotopes; D026901:Membrane Transport Proteins; D051381:Rats; D000076988:Sulfate Transporters; D027981:Symporters / Q000502:physiology; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism*; D000099209:Sodium-Iodide Symporters
D002352:Carrier Proteins; D002712:Chlorides; D007454:Iodides; D007455:Iodine; D007457:Iodine Radioisotopes; D026901:Membrane Transport Proteins; D000076988:Sulfate Transporters; D027981:Symporters; D000099209:Sodium-Iodide Symporters; C085609:SLC26A4 protein, human