Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) contains two functional lytic origins of DNA replication.
We used a transient-transfection replication assay to identify two functional copies of the human herpesvirus 8 (HHV8) lytic origin of DNA replication (oriLyt). BCLB-1 cells were transfected with HHV8 subgenomic fragments containing the putative lytic origin along with a plasmid expressing viral transactivator open reading frame (ORF) 50. The HHV8 left-end oriLyt (oriLyt-L) lies between ORFs K4.2 and K5 and is composed of a region encoding various transcription factor binding sites and an A+T-rich region and a G+C repeat region. The right-end oriLyt (oriLyt-R) maps between ORF 69 and vFLIP, a region similar to the RRV oriLyt, and is an inverted duplication of oriLyt-L.
AuCoin
David P
DP
Department of Microbiology and Cell and Molecular Biology Program, University of Nevada School of Medicine, Reno, Nevada 89557, USA.
Colletti
Kelly S
KS
Xu
Yiyang
Y
Cei
Sylvia A
SA
Pari
Gregory S
GS
D002460:Cell Line; D004261:DNA Replication; D019288:Herpesvirus 8, Human / Q000235:genetics*; D017874:Immediate-Early Proteins / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D018741:Replication Origin / Q000235:genetics* / Q000502:physiology*; D015183:Restriction Mapping; D015534:Trans-Activators / Q000235:genetics; D014162:Transfection; D014764:Viral Proteins
D017874:Immediate-Early Proteins; C417604:Rta protein, Human herpesvirus 8; D015534:Trans-Activators; D014764:Viral Proteins