title: Effect of water quality on the bonding of resin to moist dentin.
abstract: OBJECTIVE
  To test the effect of different water samples, used for rinsing the dentin, following acid conditioning, on the bond strength of a resin-based composite (RBC) restorative material (Z100), to dentin using a one-bottle dentin adhesive (Single Bond).
  
  
  METHODS
  In preliminary experiments two experimental shear bond strength methods were compared, and one was selected which was deemed to permit more uniform application of the adhesive and better light curing. In the definitive experiments there were five experimental groups, according to rinsing water used (1) distilled water as control, (2) water with 3.5 ppm iodine, (3) water 3.0 ppm sodium hypochlorite, (4) water with 2% ethanol, (5) water contaminated with 6 x 10(4) cfu/mL of E. coli BH5V. For each experimental group the shear bond strength to moist dentin from 10 human intact impacted third molars was measured. The dentin samples were etched, washed and gently blotted to leave the dentin moist. Following application of the adhesive and resin, specimens were stored at 37 degrees C for 24 hours prior to testing in an Instron testing machine at a rate of 0.5 mm/minute.
  
  
  RESULTS
  Data (in MPa +/- standard deviation) were: Group 1: 21.2 +/- 4.1; Group 2: 20.8 +/- 5.6; Group 3: 21.8 +/- 6.2; Group 4: 22.9 +/- 5.9; Group 5: 19.6 +/- 5.5. These data support the null hypothesis that the water types used in this work had no significant effect on the bonding of a resin composite to dentin (P= 0.74, ANOVA).
authors.affiliation: Department of Oral Science, School of Dentistry, University of Minnesota, Minneapolis 55455, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Wahl
authors.forename: Edward C
authors.initials: EC
authors.lastname: Combe
authors.forename: Mariano A
authors.initials: MA
authors.lastname: Polack
authors.forename: Leslie V
authors.initials: LV
authors.lastname: Martens
mesh_terms: D000134:Acid Etching, Dental; D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000737:chemistry; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry*; D016001:Confidence Intervals; D001840:Dental Bonding; D003799:Dental Stress Analysis / Q000295:instrumentation; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry; D004926:Escherichia coli; D000431:Ethanol / Q000737:chemistry; D006801:Humans; D007455:Iodine / Q000737:chemistry; D012822:Silicon Dioxide; D012973:Sodium Hypochlorite / Q000737:chemistry; D013223:Statistics as Topic; D013314:Stress, Mechanical; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry*; D014871:Water Microbiology; D015040:Zirconium
chemical_list: D000891:Anti-Infective Agents, Local; D003188:Composite Resins; D017220:Dentin-Bonding Agents; C083568:Z100 composite resin; C115612:single bond; D014867:Water; D000431:Ethanol; D017438:Bisphenol A-Glycidyl Methacrylate; D012822:Silicon Dioxide; D007455:Iodine; D015040:Zirconium; D012973:Sodium Hypochlorite