Detection of Piscirickettsia salmonis in fish tissues by an enzyme-linked immunosorbent assay using specific monoclonal antibodies.
An enzyme-linked immunosorbent assay (ELISA) for the detection of Piscirickettsia salmonis in fish tissue samples was developed. The test uses a combination of different monoclonal antibodies specific against P. salmonis in the capture step of the assay. The antibodies 7G4, 6E2 and 2C1 chosen for the capture step are bound to the solid support with an adhesive protein purified from a bivalve mollusc, resulting in a high yield of adsorption and binding stability. The monoclonal antibody 7G4, used as a second antibody, is conjugated to horseradish peroxidase. The resulting ELISA test detected 7 different isolates of P. salmonis and does not cross-react with several other fish pathogens, revealing a high specificity and sensitivity. The test also detects P. salmonis in kidney tissue of infected coho salmon with 98% correlation with the immunofluorescence assay.
Aguayo
Juan
J
Bios Chile Ingeniería Genética S.A., Fundacíon Ciencia para la Vida, Nuñoa, Santiago.
Miquel
Alvaro
A
Aranki
Naddim
N
Jamett
Adolfo
A
Valenzuela
Pablo D T
PD
Burzio
Luis O
LO
D020561:Alphaproteobacteria / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002460:Cell Line; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005393:Fish Diseases / Q000175:diagnosis* / Q000382:microbiology; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000662:veterinary; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D012487:Salmonidae; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal