Glucose transporter 4 gene: association studies pertaining to alleles of two polymorphisms in extremely obese children and adolescents and in normal and underweight controls.
The human insulin-responsive glucose transporter 4 gene (GLUT4) has been related to non-insulin-dependent diabetes mellitus (NIDDM) in several studies. Obesity is commonly found in patients with NIDDM. Hence, genes involved in NIDDM might also be relevant for obesity. We have analyzed 212 extremely obese children and adolescents, 82 normal-weight students, and 94 underweight students for two single nucleotide polymorphisms (SNPs: promoter -30G/A; exon 4a: silent 2061T/C) in the vicinity of the GLUT4 by polymerase chain reaction with subsequent restriction fragment length polymorphism analyses (PCR-RFLP) or single-strand conformation polymorphism analyses (SSCP). Allele and genotype distributions were similar in all study groups (all p values > 0.05). Hence, we did not detect association of any of the analyzed SNP alleles in the GLUT4 to different weight extremes, so these seem not to be involved in weight regulation in our study groups.
Friedel
Susann
S
Clinical Research Group, Child and Adolescent Psychiatry, Philipps-University of Marburg, Germany.
Antwerpen
Benjamin
B
Hoch
Anne
A
Vogel
Constanze
C
Grassl
Wolfgang
W
Geller
Frank
F
Hebebrand
Johannes
J
Hinney
Anke
A
D000293:Adolescent; D000328:Adult; D000483:Alleles; D001483:Base Sequence; D016022:Case-Control Studies; D002648:Child; D017931:DNA Primers; D005838:Genotype; D051275:Glucose Transporter Type 4; D006801:Humans; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009124:Muscle Proteins; D009765:Obesity / Q000235:genetics*
D017931:DNA Primers; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; C495692:SLC2A4 protein, human