Glufosinate-tolerant tobacco plants directed by the promoter of adenylate kinase gene of rice.
A DNA clone containing the 5' part of the adenylate kinase (AK) gene was isolated from a rice genomic library, and its nucleotide sequence was determined. This clone consists of 5' upstream, five exons and four introns of the AK gene. All of the determined donor and receptor sites contained 'GT' and 'AG' consensus splice sequences. Transgenic tobacco plants harbouring a chimeric gene consisting of the 5' upstream sequence of the AK gene fused with the gene encoding phosphinothricin acetyl transferase were generated. They showed tolerance to glufosinate to a level four times higher than its commercial dose.
Fukuzawa
Hiromitsu
H
Department of Agricultural Chemistry, Shiraoka Research Station of Biological Science, Nissan Chemical Industries Ltd, Minamisaitama, Saitama, Japan.
Arai
Satoshi
S
Kawai-Yamada
Maki
M
Das
Avijit
A
Tagawa
Michito
M
Uchimiya
Hirofumi
H
D000263:Adenylate Kinase / Q000235:genetics*; D000613:Aminobutyrates / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004351:Drug Resistance / Q000235:genetics; D005091:Exons; D006540:Herbicides / Q000494:pharmacology*; D007438:Introns; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics; D014170:Transformation, Genetic
D000613:Aminobutyrates; D006540:Herbicides; C003121:phosphinothricin; D000263:Adenylate Kinase