Identification of a novel anti-ice-nucleating polysaccharide from Bacillus thuringiensis YY529.
Strain YY529, capable of producing some anti-ice-nucleating materials (ANM), was isolated from the surface of a camphor leaf. Strain YY529 was identified as Bacillus thuringiensis from its characteristics and taxonomy; the optimum temperature and pH for producing these ANMs were 30 degrees C and 7.0, respectively. One of the ANM with the highest activities among them was purified from the culture. The molecular weight of the ANM was approximately 130 kDa based on a gel filtration analysis. We confirmed that this ANM was a polysaccharide based on the results of the treatment with a mannosidase and the molish reaction. In addition, the LCMS analysis showed that this anti-ice-nucleating polysaccharide (ANPS) had the polyacetyl-D-glucosamine moiety in its structure. Furthermore, this ANPS showed its ability as a non-freeze agent in a preservative solution for the cryopreservation of cock liver. This is the first report of ANPS as a novel ANM from Bacillus thuringiensis YY529.
Yamashita
Yasuhiro
Y
Department of Biotechnology, Faculty of Engineering, Kansai University, Suita City, Osaka, Japan. gm9d606@ipcku.kansai-u.ac.jp
Kawahara
Hidehisa
H
Obata
Hitoshi
H
D001413:Bacillus thuringiensis / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D004586:Electrophoresis; D013058:Mass Spectrometry / Q000379:methods; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D011135:Polysaccharides, Bacterial