Measurement of resistant starch.
A robust and reliable method was developed to measure resistant starch (RS), i.e., starch that enters the large intestine. In vivo conditions were reflected as much as possible while a user-friendly format was maintained. Parameters investigated included a-amylase concentration, pH of incubation, maltose inhibition of alpha-amylase, the need for amyloglucosidase inclusion, the effect of shaking and stirring on determined values, and problems in recovering and analyzing the RS-containing pellet. The RS values obtained were in good agreement with published in vivo data. An interlaboratory evaluation of the method has been completed (First Action Method 2002.02).
McCleary
Barr V
BV
Megazyme International Ireland Ltd, Bray, County Wicklow. barry@megazyme.com
Monaghan
Dympna A
DA
D004043:Dietary Fiber / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008320:Maltose / Q000494:pharmacology; D013213:Starch / Q000032:analysis*
D004043:Dietary Fiber; D008320:Maltose; D013213:Starch