Characterization of cloned mouse Na+/taurocholate cotransporting polypeptide by transient expression in COS-7 cells.
The mouse Na+/taurocholate cotransporting polypeptide transiently expressed in COS-7 cells caused sodium-dependent uptake of [3H]taurocholic acid with Km and Vmax values of 18 microM and 102 pmol/mg protein/min, respectively. This Km value is comparable to that for rat NTCP and higher than that for human NTCP. Substrate specificity was evaluated by measuring inhibitory effects of unlabeled bile acids on [3H]taurocholic acid transport.
Saeki
Tohru
T
Department of Biological Resource Chemistry, Faculty of Agriculture, Kyoto Prefectural University, Japan. tsaeki@kpu.ac.jp
Takahashi
Noriki
N
Kanamoto
Ryuhei
R
Iwami
Kimikazu
K
D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D006801:Humans; D051379:Mice; D029363:Organic Anion Transporters, Sodium-Dependent; D051381:Rats; D027981:Symporters
D002352:Carrier Proteins; D017931:DNA Primers; D029363:Organic Anion Transporters, Sodium-Dependent; D027981:Symporters; C071908:sodium-bile acid cotransporter