Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
A comprehensive study of Bacillus subtilis gene expression patterns in response to amino acid availability was performed by means of proteomics and transcriptomics. The methods of two-dimensional protein gel electrophoresis and DNA macroarray technology were combined to analyze cells exponentially grown in minimal medium with and without 0.2% Casamino Acids (CAA). This approach revealed about 120 genes predominantly involved in amino acid biosynthesis, sporulation, and competence, which were downregulated in CAA-containing medium. Determination of sporulation frequencies confirmed the physiological relevance of the expression data.
Mäder
Ulrike
U
Institut für Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, D-17487 Greifswald, Germany. ulrike.maeder@uni-greifswald.de
Homuth
Georg
G
Scharf
Christian
C
Büttner
Knut
K
Bode
Rüdiger
R
Hecker
Michael
M
D000596:Amino Acids / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome / Q000032:analysis / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D013171:Spores, Bacterial / Q000254:growth & development
D000596:Amino Acids; D001426:Bacterial Proteins; D003470:Culture Media; D020543:Proteome; D012329:RNA, Bacterial; D012333:RNA, Messenger; C017721:casamino acids