title: Interleukin-10 promoter polymorphisms and cervical cancer risk in Korean women.
abstract: The aims of this study were to determine whether polymorphisms of the interleukin (IL)-10 promoter might be associated with an increased risk of cervical cancer, and further whether systemic IL-10 concentration might be influenced by the genotypes in Korean women. Peripheral blood samples from patients with invasive cervical cancer (ICC, n=144) and non-cancer controls (NCC, n=179) were used to detect three biallelic IL-10 promoter polymorphisms at -1082, -819, and -592 sites by polymerase chain reaction-restriction fragment length polymorphism assay using MnlI, MaeIII and RsaI, respectively. The IL-10 serum concentration was measured with enzyme-linked immunosorbent assay. We compared the distribution of genotypes in both groups, evaluated the serum IL-10 level according to the genotypes, and analyzed the association of these polymorphisms with the risk of cervical cancer. The genotype at the -1082 site exhibited only the *A homozygote, and only two haplotypes were found in Korean women, one being -1082*A/-819*T/-592*A (ATA) and the other -1082*A/-819*C/-592*C (ACC). No association was found for IL-10 promoter polymorphisms among the ICC patients in comparison with the NCC subjects. The risk of cervical cancer was not increased in either genotype and the IL-10 serum concentration was not influenced by the genotypes in either group. Polymorphisms of the IL-10 promoter do not appear to be associated with cervical cancer risk or systemic IL-10 production in Korean women.
authors.affiliation: Center for Uterine Cancer, National Cancer Center, Goyang, Gyeonggi 411-764, South Korea.
authors.forename: Ju Won
authors.initials: JW
authors.lastname: Roh
authors.forename: Moon Hong
authors.initials: MH
authors.lastname: Kim
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Seo
authors.forename: Su Hyeong
authors.initials: SH
authors.lastname: Kim
authors.forename: Jae Weon
authors.initials: JW
authors.lastname: Kim
authors.forename: Noh Hyun
authors.initials: NH
authors.lastname: Park
authors.forename: Yong Sang
authors.initials: YS
authors.lastname: Song
authors.forename: Sang Yoon
authors.initials: SY
authors.lastname: Park
authors.forename: Soon Beom
authors.initials: SB
authors.lastname: Kang
authors.forename: Hyo Pyo
authors.initials: HP
authors.lastname: Lee
mesh_terms: D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D006720:Homozygote; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D007723:Korea / Q000453:epidemiology; D009361:Neoplasm Invasiveness / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000235:genetics*; D016387:Women's Health
chemical_list: D005819:Genetic Markers; D016753:Interleukin-10