title: In vitro anti-Neisseria gonorrhoeae activity of Terminalia macroptera leaves.
abstract: We used the agar dilution method to evaluate the antibacterial effect of Terminalia macroptera leaf (Tml) extract against nine reference and clinical Neisseria gonorrhoeae strains, including penicillin- and tetracycline-resistant and -susceptible strains. Tml possesses anti-N. gonorrhoeae activity against all of the strains and the minimum inhibitory concentrations (MIC) were between 100 and 200 microg ml(-1). We then used a liquid-liquid partition method to divide the Tml extract into five fractions and determined the anti-N. gonorrhoeae activity of each of the fractions. All of the fractions showed antibacterial activity. The most active one was identified as the diethyl ether fraction and had MIC values of between 25 and 50 microg ml(-1) against all of the strains.
authors.affiliation: CECF, Laboratory of Pharmacognosy, Faculty of Pharmacy, University of Lisbon, Av. das Forças Armadas, 1649-019, Lisbon, Portugal.
authors.forename: Olga
authors.initials: O
authors.lastname: Silva
authors.forename: Eugénia
authors.initials: E
authors.lastname: Ferreira
authors.forename: M
authors.initials: M
authors.lastname: Vaz Pato
authors.forename: Manuela
authors.initials: M
authors.lastname: Caniça
authors.forename: Elsa T
authors.initials: ET
authors.lastname: Gomes
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001578:Benzopyrans / Q000737:chemistry; D004610:Ellagic Acid / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry; D005707:Gallic Acid / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry; D047348:Hydrolyzable Tannins; D047311:Luteolin; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D013634:Tannins / Q000737:chemistry; D029766:Terminalia / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001578:Benzopyrans; D005419:Flavonoids; D005960:Glucosides; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; D013634:Tannins; C103481:chebulinic acid; D004610:Ellagic Acid; C076178:chebulagic acid; D005707:Gallic Acid; C115642:punicalagin; C057912:homoorientin; D047311:Luteolin