Effect of culture conditions on Escherichia coli O157:H7-mediated attaching-effacing lesions in a bovine large intestinal mucosal explant model.
The effects of culture conditions on the extent of Escherichia coli O157:H7 attaching-effacing (A/E) adherence in an adult bovine large intestinal mucosal explant model were assessed by three different morphometric methods. Measurement of the percent of tissue sections with A/E adherence and the number of foci of A/E adherence mm(-1) of surface epithelium was more sensitive than measurement of the percent of surface epithelium with A/E adherent bacteria for detection of treatment effects. Culture of bacterial inoculum in tryptic soy broth, incubation of explants in 5% CO(2), and rocking of explants on a platform rocker at 18 cycles min(-1) provided optimal conditions for A/E adherence. In future studies, the model may be used for preliminary testing of intervention strategies aimed at reduction of E. coli O157:H7 intestinal colonization of cattle.
Baehler
Angela A
AA
Department of Veterinary and Biomedical Sciences, Institute of Agriculture and Natural Resources, 111 VBS, University of Nebraska-Lincoln, Fair St. and East Campus Loop, Lincoln, NE 68583-0905, USA.
Moxley
Rodney A
RA
D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology / Q000473:pathology*; D003470:Culture Media; D004927:Escherichia coli Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000473:pathology; D007420:Intestine, Large / Q000382:microbiology / Q000473:pathology; D009924:Organ Culture Techniques / Q000379:methods
D003470:Culture Media