title: Interleukin-1beta stimulates interleukin-8 production and gene expression in synovial cells from human temporomandibular joint.
abstract: OBJECTIVE
  The aims of the present study were to isolate and characterize cultured synovial cells from human temporomandibular joint (TMJ) specimens and to investigate the effect of interleukin (IL)-1beta on IL-8 production and gene expression in those cells.
  
  
  METHODS
  Synovial cells (HTS cells) were isolated from TMJ synovial tissues using an outgrowth method and then primary cultured. The cells were examined for cell-specific markers of fibroblast, macrophage, and dendritic cells using immunocytochemistry. HTS cells were then treated with IL-1beta, and amounts of IL-8 were measured by enzyme-linked immunosorbent assay. IL-8 production and expression were also investigated using immunocytochemistry and a reverse transcription-polymerase chain reaction method.
  
  
  RESULTS
  HTS cells were positive for the fibroblast-specific markers, such as vimentin and propyl 4-hydroxylase. The macrophage or dendritic cell markers and HLA class II antigen were negative. Furthermore, IL-1beta enhanced IL-8 production in HTS cells in a time- and dose-dependent manner and stimulated IL-8 gene expression.
  
  
  CONCLUSIONS
  HTS cells may provide important advantages for studies of the cellular and molecular mechanisms in the TMJ. In addition, we found that IL-1beta stimulated IL-8 production through an increase in IL-8 gene expression in HTS cells, which may be associated with the increase of infiltrating inflammatory cells seen in the synovial membrane of TMJ disorders.
authors.affiliation: Department of Oral Surgery, Nihon University School of Dentistry at Matsudo, 2-870-1, Sakaecho-Nishi, Matsudo, Chiba 271-8587, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Tobe
authors.forename: Naomi
authors.initials: N
authors.lastname: Ogura
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Abiko
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nagura
mesh_terms: D000328:Adult; D000844:Ankylosis / Q000473:pathology; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000032:analysis; D002478:Cells, Cultured; D004396:Coloring Agents; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000494:pharmacology*; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D004204:Joint Dislocations / Q000473:pathology; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013223:Statistics as Topic; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism*; D013704:Temporomandibular Joint / Q000166:cytology / Q000378:metabolism*; D019224:Temporomandibular Joint Disc / Q000473:pathology; D013705:Temporomandibular Joint Disorders / Q000473:pathology; D013997:Time Factors; D014746:Vimentin / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D004396:Coloring Agents; D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D016209:Interleukin-8; D012333:RNA, Messenger; D014746:Vimentin; D006899:Mixed Function Oxygenases