Effect of caffeic acid on apical transporters' dysfunction of renal proximal tubule cells under oxidative stress in vitro.
The protective effect of caffeic acid (CA) against oxidative stress-induced inhibition of proximal tubule apical transporter was investigated. In the present study, 10 (-4) M H2O2 did not affect cell viability regardless of incubation time. However, it decreased apical transporters' activity such as Na (+)/glucose cotransporter, Na (+)/Pi cotransporter, and Na (+)/H(+) antiporter in the proximal tubule cells. CA (>10(-6) M) prevented H2O2-induced inhibition of apical transporters. Thus, we investigated its action mechanism. CA also prevented H2O2-induced lipid peroxides formation, arachidonic acid (AA) release, and Ca(2+) uptake. In conclusion, CA, in part, prevented H2O2-induced inhibition of apical transporter activity via decrease of AA release and Ca(2+) uptake in primary cultured renal proximal tubule cells.
Han
Ho Jae
HJ
College of Veterinary Medicine, Biotechnology Research Institute, Chonnam National University, Kwangju, Korea. hjhan@chonnam.ac.kr
Park
Soo Hyun
SH
Park
Kwon Moo
KM
Yoon
Byung Cheol
BC
Kim
Tae Sung
TS
Lee
Jang Hern
JH
D000818:Animals; D016718:Arachidonic Acid / Q000493:pharmacokinetics; D002109:Caffeic Acids / Q000494:pharmacology*; D002118:Calcium / Q000493:pharmacokinetics; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D026901:Membrane Transport Proteins / Q000187:drug effects* / Q000378:metabolism; D008757:Methylglucosides / Q000037:antagonists & inhibitors; D009004:Monosaccharide Transport Proteins / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010710:Phosphates / Q000037:antagonists & inhibitors; D011817:Rabbits; D012964:Sodium / Q000493:pharmacokinetics; D017923:Sodium-Hydrogen Exchangers / Q000187:drug effects / Q000378:metabolism; D050608:Sodium-Phosphate Cotransporter Proteins; D027981:Symporters / Q000187:drug effects / Q000378:metabolism
D002109:Caffeic Acids; D026901:Membrane Transport Proteins; D008757:Methylglucosides; D009004:Monosaccharide Transport Proteins; D010710:Phosphates; D017923:Sodium-Hydrogen Exchangers; D050608:Sodium-Phosphate Cotransporter Proteins; D027981:Symporters; D016718:Arachidonic Acid; C027020:methylglucoside; D012964:Sodium; D006861:Hydrogen Peroxide; D002118:Calcium; C040048:caffeic acid