Spectroelectrochemistry study on the electrochemical reduction of ethidium bromide.
The electrochemical reduction mechanism of ethidium bromide was first studied by spectroelectrochemistry. This reduction was proved to be a two-step process by cyclic voltammetry, differential pulse voltammetry and spectroelectrochemistry, in which each step was proved to be a one-electron transfer process by a spectropotentiostatic fluorescence technique. Hydroethidine was confirmed to be the final product by comparing the spectrum of the product of the electrochemical reduction to that of the product of the chemical reduction of ethidium bromide, and a carbon-centered radical was concluded to be a reasonable intermediate product during the electrochemical reduction of ethidium bromide.
Hu
Xinhui
X
Department of Chemistry, East China Normal University, Shanghai.
Wang
Qingjiang
Q
He
Pingang
P
Fang
Yuzhi
Y
D001559:Benzhydryl Compounds / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004579:Electron Transport; D004583:Electrons; D004996:Ethidium / Q000737:chemistry*; D005456:Fluorescent Dyes; D005609:Free Radicals / Q000737:chemistry; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010617:Phenanthridines / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods*; D014320:Triphenylmethyl Compounds / Q000737:chemistry
D001559:Benzhydryl Compounds; D005456:Fluorescent Dyes; D005609:Free Radicals; D010617:Phenanthridines; D014320:Triphenylmethyl Compounds; C051224:methyl radical; C058428:hydroethidine; D004996:Ethidium; D008697:Methane