title: Free radical scavengers, anti-inflammatory and analgesic activity of Acaena magellanica.
abstract: Extracts of the whole plant Acaena magellanica (Rosaceae) were assessed for anti-inflammatory, antipyretic and analgesic activity in animal models. At 600 mg kg(-1), the global ethanolic extract (GEE), dichloromethane (DCM) and defatted methanol (MeOH) fractions showed a mild anti-inflammatory effect in the carrageenan-induced guinea-pig paw oedema. The GEE, DCM and defatted MeOH fractions significantly reduced inflammation by 43.2, 40.5 and 42.1%, respectively. The GEE did not showed any significant antipyretic activity in doses up to 600 mg kg(-1). A 20% w/v infusion administered orally at 16 mL kg(-1) presented analgesic effect in the acetic acid-induced abdominal constriction test in mice. The GEE and MeOH extract of A. magellanica showed free radical scavenging activity in the diphenylpicrylhydrazyl decolouration assay. Assay-guided isolation led to quercetin, Q-3-O-beta-glucoside, Q-3-O-beta-D-galactoside, ellagic acid and catechin as the free radical scavengers. The saponins tormentic acid 28-O-beta-D-galactopyranoside and 28-O-beta-D-glucopyranoside were isolated from the polar extract. The structures were determined by spectroscopic methods.
authors.affiliation: Institute de Ciencias Básicas, Universidad Nacional de San Juan, Argentina.
authors.forename: Gabriela Egly
authors.initials: GE
authors.lastname: Feresin
authors.forename: Alejandro
authors.initials: A
authors.lastname: Tapia
authors.forename: Angel
authors.initials: A
authors.lastname: Gutierrez R
authors.forename: Carla
authors.initials: C
authors.lastname: Delporte
authors.forename: Nadine
authors.initials: N
authors.lastname: Backhouse Erazo
authors.forename: Guillermo
authors.initials: G
authors.lastname: Schmeda-Hirschmann
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004487:Edema / Q000517:prevention & control; D005260:Female; D005334:Fever / Q000517:prevention & control; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D006168:Guinea Pigs; D008297:Male; D051379:Mice; D023421:Models, Animal; D010147:Pain Measurement; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D011817:Rabbits; D027824:Rosaceae / Q000737:chemistry*; D023382:Toxicity Tests, Acute
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016166:Free Radical Scavengers; D010936:Plant Extracts