title: Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli.
abstract: A yedU gene product with a molecular mass of 31 kDa is a hypothetical protein with no known function. The protein was purified and crystallized at 296 K. X-ray diffraction data have been collected to 2.3 A using synchrotron radiation. The crystals belong to the primitive orthorhombic system, with unit-cell parameters a = 50.56, b = 63.45, c = 168.02 A. The asymmetric unit contains two monomers of the protein, with a corresponding V(M) of 2.25 A(3) Da(-1) and a solvent content of 44.84%.
authors.affiliation: Beamline Division, Pohang Accelerator Laboratory, Pohang, Kyungbuk 790-784, South Korea.
authors.forename: Ok-Gwan
authors.initials: OG
authors.lastname: Kim
authors.forename: In-Kwon
authors.initials: IK
authors.lastname: Kim
authors.forename: Ghyung-Hwa
authors.initials: GH
authors.lastname: Kim
authors.forename: Junsang
authors.initials: J
authors.lastname: Ko
authors.forename: Chankyu
authors.initials: C
authors.lastname: Park
authors.forename: Pann-Ghill
authors.initials: PG
authors.lastname: Suh
authors.forename: Sa-Ouk
authors.initials: SO
authors.lastname: Kang
authors.forename: Heung-Soo
authors.initials: HS
authors.lastname: Lee
authors.forename: Sun-Shin
authors.initials: SS
authors.lastname: Cha
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D018832:Molecular Chaperones / Q000737:chemistry*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D017356:Synchrotrons
chemical_list: D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D011994:Recombinant Proteins; C469996:hchA protein, E coli