title: [Detection of Brugia malayi larva in mosquito vector by PCR and PCR-ELISA].
abstract: OBJECTIVE
  To develop sensitive, specific, simple assays for the detection of Brugia malayi larva in mosquito vectors.
  
  
  METHODS
  The optimum conditions for PCR and PCR-ELISA were determined. With dissected larvae, detection limits and as well as specificities of the two assays were worked out. The optimized assays were then tested with infected and non-infected mosquito vectors, Anopheles sinensis.
  
  
  RESULTS
  Both PCR and PCR-ELISA detected DNA equivalent to a single L1-stage larva(L1). While the actual detecting limits of the two, as estimated and titrated respectively, reached to 1/10 and 1/100 of a L1. When the two assays were used to detect the larvae isolated from 113 infected A. sinensis, all gave specific bands and positive reactions. However, trials on direct amplifications with crude extracts of infected mosquito vectors consistently failed to give specific bands upon electrophoresis and negative results in PCR-ELISA as well.
  
  
  CONCLUSIONS
  Both PCR and PCR-ELISA were preliminarily established for the detection of isolated B. malayi larva in mosquito vector, which proved to be sensitive, specific and easy to manipulate.
authors.affiliation: Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: D
authors.initials: D
authors.lastname: Sun
authors.forename: H
authors.initials: H
authors.lastname: Xue
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D017178:Brugia malayi / Q000302:isolation & purification*; D018509:DNA, Helminth / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D007303:Insect Vectors / Q000469:parasitology*; D007814:Larva; D016133:Polymerase Chain Reaction
chemical_list: D018509:DNA, Helminth