Suppression of metastasis of rat prostate cancer by introduction of human chromosome 13.
OBJECTIVE
Chromosome 13 is one of the most frequently altered chromosomes in prostate cancer. The present study was undertaken to examine the role of human chromosome 13 in the progression of prostate cancer.


METHODS
Human chromosome 13 was introduced into highly metastatic rat prostate cancer cells via microcell-mediated chromosome transfer.


RESULTS
Microcell hybrid clones containing human chromosome 13 showed suppression of metastasis to the lung without any suppression of tumorigenicity, except for one clone, which contained the smallest sized human chromosome 13 and did not show any suppression on lung metastasis. Expression of two known tumor suppressor genes, BRCA2 and RB1, which map to chromosome 13, was examined by reverse transcription- polymerase chain reaction analysis. BRCA2 was expressed only in the metastasis-suppressed microcell-hybrid clones, whereas RB1 was expressed in all clones.


CONCLUSIONS
Human chromosome 13 contains metastasis suppressor gene(s) for prostate cancer derived from rat. Furthermore, the RB1 gene is unlikely to be involved in the suppression of metastasis evident in this system.
Hosoki
Shigeru
S
Department of Urology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
Ota
Sho
S
Ichikawa
Yayoi
Y
Suzuki
Hiroyoshi
H
Ueda
Takeshi
T
Naya
Yukio
Y
Akakura
Koichiro
K
Igarashi
Tatsuo
T
Oshimura
Mitsuo
M
Nihei
Naoki
N
Barrett
J Carl
JC
Ichikawa
Tomohiko
T
Ito
Haruo
H
D000818:Animals; D030801:Animals, Genetically Modified; D002455:Cell Division / Q000235:genetics; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D018450:Disease Progression; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007700:Kinetics; D008297:Male; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000517:prevention & control; D051381:Rats / Q000235:genetics*
D005819:Genetic Markers