title: Fish test for endocrine-disruption and estimation of water quality of Japanese rivers.
abstract: The LC50 values (72 h) of 17beta-estradiol (E2), p-nonylphenol (NP) and bis-phenol-A (BPA) to adult male and female medaka were 3.5 and 3.5, 0.85 and 0.87, and 6.8 and 8.3 mg L(-1), respectively; the LC50 values to embryos were 0.46, 0.13 and 5.1 mg L(-1), respectively. The IC50 values for inhibition to egg hatching were 0.47, 0.85 and 9.0 mg L(-1), respectively. These values were much higher than concentrations detected in river water in Japan and the chemicals were considered to have no lethal effect on the fish in an aquatic environment. Mature male medaka was continuously exposed to 0.005, 0.05 or 1.0 microg L(-1) of E2, or to 0.1, 10 or 100 microg L(-1) of NP or BPA. Female specific proteins (FSP) were induced in the blood of male medaka that were exposed for 5 weeks to E2 higher than 0.005 microg L(-1), NP higher than 0.1 microg L(-1), or BPA higher than 10 microg L(-1). Based on these FSP inducible concentrations and reported concentrations of E2, NP and BPA in Japanese river water, some river water contaminated by E2 or NP could be estimated as the FSP inducible in male medaka.
authors.affiliation: Institute of Environmental Ecology, Metocean Environment Inc., Shizuoka, Japan. shosaku@duke.edu
authors.forename: Shosaku
authors.initials: S
authors.lastname: Kashiwada
authors.forename: Hidenori
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Nobukazu
authors.initials: N
authors.lastname: Miyamoto
authors.forename: Yuta
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Yasumoto
authors.initials: Y
authors.lastname: Magara
mesh_terms: D000395:Air Pollutants, Occupational / Q000009:adverse effects*; D000818:Animals; D001559:Benzhydryl Compounds; D004305:Dose-Response Relationship, Drug; D004531:Eggs; D004703:Endocrine System / Q000187:drug effects; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods*; D004958:Estradiol / Q000009:adverse effects*; D005260:Female; D005399:Fishes; D007564:Japan; D007928:Lethal Dose 50; D008297:Male; D010636:Phenols / Q000009:adverse effects*; D012098:Reproduction / Q000187:drug effects; D014819:Vitellogenins / Q000096:biosynthesis* / Q000187:drug effects; D000098826:Bisphenol A Compounds
chemical_list: D000395:Air Pollutants, Occupational; D001559:Benzhydryl Compounds; D004958:Estradiol; D010636:Phenols; D014819:Vitellogenins; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds