Alterations of motor nerve functions in animals exposed to motorcycle exhaust.
The effect of motorcycle exhaust (ME) on the motor nerve was studied using animals exposed to the exhaust by inhalation, intratracheal, or intraperitoneal administration of ME particulate (MEP). A 4-wk ME inhalation and intratracheal instillation of MEP for 1 d in rats or intraperitoneal administration of MEP (0.5 g/kg/d for 1 d and 0.1 g/kg/d for 2 d) in mice significantly decreased both rota-rod performance and motor nerve conduction velocity. The effect of some polycyclic aromatic hydrocarbons on the motor nerve was also investigated. Treatment with benzo[a]pyrene (0.05 and 0.1 g/kg/d for 3 d), but not pyrene (0.1 g/kg/d for 3 d), resulted in significant decrease of motor nerve conduction velocity in mice. Moreover, the Na(+),K(+)-ATPase activities of sciatic nerves isolated from ME-, MEP-, or benzo[a]pyrene-exposed animals were decreased. Treatment with pyrene did not markedly affect the Na(+),K(+)-ATPase activity of sciatic nerve. The rats exposed to ME for 4 wk showed increases in blood and sciatic nerve manganese levels. Results indicate that motorcycle exhaust produces adverse effects on the motor nerve, which is associated with a fall in nerve Na(+),K(+)-ATPase activity.
Liu
Shing-Hwa
SH
Institute of Toxicology, Medical College, National Taiwan University, Taipei, Taiwan. shliu@ha.mc.ntu.edu.tw
Wang
Jei-Hui
JH
Chuu
Jiunn-Jye
JJ
Lin-Shiau
Shoei-Yn
SY
D000280:Administration, Inhalation; D000818:Animals; D001564:Benzo(a)pyrene / Q000008:administration & dosage / Q000009:adverse effects; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D007442:Intubation, Intratracheal; D008297:Male; D008345:Manganese / Q000097:blood / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009048:Motor Skills / Q000187:drug effects; D009049:Motorcycles; D009431:Neural Conduction / Q000187:drug effects*; D011084:Polycyclic Aromatic Hydrocarbons / Q000008:administration & dosage / Q000009:adverse effects*; D011721:Pyrenes / Q000008:administration & dosage / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects* / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D001335:Vehicle Emissions / Q000009:adverse effects*
D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D001335:Vehicle Emissions; D001564:Benzo(a)pyrene; D008345:Manganese; C030984:pyrene; D000254:Sodium-Potassium-Exchanging ATPase