title: Induction of MCP-1 expression in airway epithelial cells: role of CCR2 receptor in airway epithelial injury.
abstract: The repair of an injured bronchial epithelial cell (BEC) monolayer requires proliferation and migration of BECs into the injured area. We hypothesized that BEC monolayer injury results in monocyte chemoattractant protein-1 (MCP-1) production, which initiates the repair process. BECs (BEAS-2B from ATCC) were utilized in this study. MCP-1 interacts with CCR2B receptor (CCR2B), resulting in cell proliferation, haptotaxis, and healing of the monolayer. Reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to verify the presence of CCR2B. CCR2B was not merely present but also inducible by interleukin-2 (IL-2) and lipopolysaccharide (LPS). We demonstrated by immunohistochemistry that BECs express MCP-1 after injury and that receptor expression can be regulated by exposure to IL-2 and LPS. Haptotactic migration of cells was enhanced in the presence of MCP-1 and reduced in the presence of CCR2B antibody. This enhanced or depressed ability of the BECs to perform haptotactic migration was shown to be statistically significant (P < 0.05) when compared to controls. Finally, BECs proliferate in response to MCP-1 as proven by electric cell-substrate impedance sensing (ECIS) technology. MCP-1-specific antibodies were shown to neutralize the MCP-1-mediated BEC proliferation. This cascade of events following injury to the bronchial epithelium may provide insight into the mechanism of the repair process.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Veterans Affairs Medical Center, Indiana University School of Medicine, Indianapolis 46202, USA.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Lundien
authors.forename: Kamal A
authors.initials: KA
authors.lastname: Mohammed
authors.forename: Najmunnisa
authors.initials: N
authors.lastname: Nasreen
authors.forename: R S
authors.initials: RS
authors.lastname: Tepper
authors.forename: Joyce A
authors.initials: JA
authors.lastname: Hardwick
authors.forename: Kerry L
authors.initials: KL
authors.lastname: Sanders
authors.forename: Robert D
authors.initials: RD
authors.lastname: Van Horn
authors.forename: Veena B
authors.initials: VB
authors.lastname: Antony
mesh_terms: D001980:Bronchi / Q000166:cytology*; D002455:Cell Division; D002461:Cell Line, Transformed; D002465:Cell Movement; D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology*; D005786:Gene Expression Regulation; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D054390:Receptors, CCR2; D019707:Receptors, Chemokine / Q000187:drug effects / Q000502:physiology
chemical_list: C515334:CCR2 protein, human; D018932:Chemokine CCL2; D007376:Interleukin-2; D008070:Lipopolysaccharides; D054390:Receptors, CCR2; D019707:Receptors, Chemokine