title: Toxicokinetics, recovery, and metabolism of triclopyr butotyl (ACTP) ester in goats.
abstract: Toxicokinetic behavior, recovery, and metabolism studies of ACTP ester and its effect on cytochrome P(450) content of liver microsomal pellet were carried out in black Bengal goat after a single intravenous administration of 11.88 mg kg(-1) and consecutive oral administration of 79.22 mg kg(-1) for 7 days. ACTP ester achieved a maximum blood concentration of 42.64 +/- 4.26 microg mL(-1) at 0.08 h after intravenous administration followed by a sharp decline until 0.5 h, and the minimum blood concentration was recorded at 36 h (1.93 +/- 0.14 microg mL(-1)) postdosing. The kinetic behavior of ACTP ester followed a "two-compartment open model". Comparatively shorter alpha (0.81 +/- 0.02 h(-1)) and greater t1/2 (alpha) (0.86 +/- 0.03 h) indicated a slower rate of distribution of ACTP ester in goat. The t1/2(beta)()) (14.83 +/- 1.49 h) and V(d(area)) (0.91 +/- 0.19 L kg(-1)) suggested a longer elimination phase with general distribution in all compartments of the body. The higher T/B and K12/K21 values associated with a lower f(c) value suggested longer persistence in the tissue compartment at higher concentration. The higher Cl(R) compared to Cl(H) indicated the major amount was eliminated by the kidney. Maximum concentration of ACTP ester including its metabolites, triclopyr acid and trichloropyridinol, was excreted through urine at 48 h. The recovery of ACTP ester including metabolites after repeated nontoxic oral dose administration was 70.09%, of which recovery from feces was 4.45%, suggesting the major portion of administered ACTP ester was absorbed through the gastrointestinal tract of the goat. All of the tissues contained ACTP ester and its metabolites. ACTP ester did not alter the cytochrome P(450) content of the liver tissue following repeated nontoxic oral dose administration for 7 days.
authors.affiliation: Department of Pharmacology and Toxicology, West Bengal University of Animal and Fishery Sciences, Mohanpur, Nadia 741 252, West Bengal, India.
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Sar
authors.forename: Biplab
authors.initials: B
authors.lastname: Bagchi
authors.forename: Shyamal K
authors.initials: SK
authors.lastname: Das
authors.forename: Tapan K
authors.initials: TK
authors.lastname: Mandal
authors.forename: Animesh K
authors.initials: AK
authors.lastname: Chakraborty
authors.forename: Anjan
authors.initials: A
authors.lastname: Bhattacharyya
authors.forename: Ashim
authors.initials: A
authors.lastname: Choudhury
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004064:Digestive System / Q000737:chemistry; D005243:Feces / Q000737:chemistry; D005260:Female; D006016:Glycolates / Q000032:analysis / Q000493:pharmacokinetics* / Q000633:toxicity*; D006041:Goats / Q000378:metabolism*; D006540:Herbicides / Q000032:analysis / Q000493:pharmacokinetics* / Q000633:toxicity*; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology
chemical_list: D006016:Glycolates; D006540:Herbicides; D003577:Cytochrome P-450 Enzyme System; C032742:triclopyr