Phenylethylamine induces an increase in cytosolic Ca2+ in yeast.
Beta-phenylethylamine (PEA) induced an increase in cytosolic free calcium ion concentration ([Ca2+]c) in Saccharomyces cerevisiae cells monitored with transgenic aequorin, a Ca2+-dependent photoprotein. The PEA-induced [Ca2+]c increase was dependent on the concentrations of PEA applied, and the Ca2+ mostly originated from an extracellular source. Preceding the Ca2+ influx, H2O2 was generated in the cells by the addition of PEA. Externally added H2O2 also induced a [Ca2+]c increase. These results suggest that PEA induces the [Ca2+]c increase via H2O2 generation. The PEA-induced [Ca2+]c increase occurred in the mid1 mutant with a slightly smaller peak than in the wild-type strain, indicating that Mid1, a stretch-activated nonselective cation channel, may not be mainly involved in the PEA-induced Ca2+ influx. When PEA was applied, the MATa mid1 mutant was rescued from alpha-factor-induced death in a Ca2+-limited medium, suggesting that the PEA-induced [Ca2+]c increase can reinforce calcium signaling in the mating pheromone response pathway.
Pinontoan
Reinhard
R
Nagoya University Bioscience Center, Nagoya University, Japan.
Krystofova
Svetlana
S
Kawano
Tomonori
T
Mori
Izumi C
IC
Tsuji
Frederick I
FI
Iida
Hidetoshi
H
Muto
Shoshi
S
D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects* / Q000378:metabolism; D000072235:Mating Factor; D010455:Peptides / Q000378:metabolism; D010627:Phenethylamines / Q000494:pharmacology*; D010675:Pheromones / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism
D010455:Peptides; D010627:Phenethylamines; D010675:Pheromones; C029261:phenethylamine; D000072235:Mating Factor; D002118:Calcium