title: Methods for cultivation of luminal parasitic protists of clinical importance.
abstract: Cultivation of luminal protistan parasites has a long history. In this review we discuss the methods and media that are most widely used for the establishment and maintenance of the following organisms in culture: Entamoeba histolytica, Giardia intestinalis, Trichomonas vaginalis, Dientamoeba fragilis, Blastocystis hominis, and Balantidium coli. While cultivation is of limited importance in the diagnostic laboratory, it is essential to most research laboratories, and it is toward the latter that this review is primarily aimed.
authors.affiliation: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, Great Britain. graham.clark@lshtm.ac.uk
authors.forename: C Graham
authors.initials: CG
authors.lastname: Clark
authors.forename: Louis S
authors.initials: LS
authors.lastname: Diamond
mesh_terms: D000818:Animals; D003470:Culture Media; D056890:Eukaryota / Q000254:growth & development* / Q000302:isolation & purification; D049673:History, 20th Century; D006801:Humans; D010274:Parasitology / Q000266:history / Q000379:methods; D011528:Protozoan Infections / Q000469:parasitology
chemical_list: D003470:Culture Media