A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method.
This study describes the development of a method for the incorporation of fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes (ISCOMs) prepared by the hydration method. Conjugation of palmitic acid was performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA, with subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both pFITC-OVA and FITC-pOVA, but not FITC-OVA, could be incorporated into ISCOMs and other non-liposomal colloidal structures. The degree of incorporation of pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if ISCOMs are the predominant colloids in the system.
Könnings
Stephanie
S
Colloidal Drug Delivery Group, School of Pharmacy, University of Otago, P.O. Box 913, Dunedin, New Zealand.
Copland
Melissa J
MJ
Davies
Nigel M
NM
Rades
Thomas
T
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry; D010047:Ovalbumin / Q000008:administration & dosage* / Q000737:chemistry; D062605:Quillaja Saponins; D012503:Saponins / Q000008:administration & dosage / Q000737:chemistry
D000276:Adjuvants, Immunologic; D005456:Fluorescent Dyes; D062605:Quillaja Saponins; D012503:Saponins; C046386:Quil A; D010047:Ovalbumin; D016650:Fluorescein-5-isothiocyanate