Specific mucosal IgA immunity in turkey poults infected with turkey coronavirus.
The objective of this study was to elucidate the kinetics and magnitudes of specific IgA antibody responses in intestines of turkey poults infected with turkey coronavirus (TCV). Turkey poults were orally inoculated with TCV at 10 days of age. Intestinal segment cultures were administered for duodenum, jejunum, and ileum and the IgA antibody responses were analyzed at 1, 2, 3, 4, 6, or 9 weeks post-infection (PI) in two different experiments. The kinetics of virus-specific IgA antibody responses in duodenum, jejunum, and ileum were similar: gradually increased from 1 week PI, reached the peak at 3 or 4 weeks PI, and declined afterward. The virus-specific IgA antibody responses in duodenum, jejunum, and ileum showed negative correlation with duration of TCV antigen in the corresponding locations of intestine with Spearman's correlation coefficient of -0.85 (p=0.034), -0.74 (p=0.096), and -0.75 (p=0.084), respectively. Moreover, the virus-specific IgA antibody responses in serum were positively correlated with that of duodenum (coefficient=0.829, p=0.042), jejunum (coefficient=0.829, p=0.042), and ileum (coefficient=0.771, p=0.072) segment cultures, suggesting that the induction of specific IgA response in serum was predictive of an IgA response in intestine. The results indicate that intestinal mucosal IgA antibodies to TCV are elicited in turkeys following infection with TCV. The local mucosal antibodies may provide protective immunity for infected turkeys to recover from TCV infection.
Loa
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Department of Veterinary Pathobiology, Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907-1175, USA.
Lin
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TL
Wu
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CC
Bryan
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Hooper
T
T
Schrader
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D
D000818:Animals; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D017934:Coronavirus / Q000276:immunology*; D004753:Enteritis, Transmissible, of Turkeys / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D018928:Immunity, Mucosal / Q000276:immunology; D007070:Immunoglobulin A / Q000096:biosynthesis / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology / Q000648:ultrastructure / Q000821:virology; D007421:Intestine, Small / Q000276:immunology* / Q000648:ultrastructure / Q000821:virology; D007700:Kinetics; D008854:Microscopy, Electron; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D014422:Turkeys
D000956:Antigens, Viral; D007070:Immunoglobulin A