title: Use of a linear gradient flow program for liquid chromatography-mass spectrometry protein-binding studies.
abstract: A rapid screening method to measure drug-protein binding using an immobilized human serum albumin (HSA) column was developed. This method utilizes a linear gradient flow-rate to accelerate the elution of strong binders to the HSA column. Post-column addition of a pressure relief valve enables mass spectrometric detection at relatively high mobile phase flow-rates (i.e., 2 ml/min).
authors.affiliation: Discovery Analytical Chemistry, Wyeth-Ayerst Research, Pearl River, NY 10965, USA. liuh2@war.wyeth.com
authors.forename: Hanlan
authors.initials: H
authors.lastname: Liu
authors.forename: Chantel
authors.initials: C
authors.lastname: Sabus
authors.forename: Guy T
authors.initials: GT
authors.lastname: Carter
authors.forename: Mark
authors.initials: M
authors.lastname: Tischler
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D011485:Protein Binding