Interleukin-10 promoter polymorphisms and cervical cancer risk in Korean women.
The aims of this study were to determine whether polymorphisms of the interleukin (IL)-10 promoter might be associated with an increased risk of cervical cancer, and further whether systemic IL-10 concentration might be influenced by the genotypes in Korean women. Peripheral blood samples from patients with invasive cervical cancer (ICC, n=144) and non-cancer controls (NCC, n=179) were used to detect three biallelic IL-10 promoter polymorphisms at -1082, -819, and -592 sites by polymerase chain reaction-restriction fragment length polymorphism assay using MnlI, MaeIII and RsaI, respectively. The IL-10 serum concentration was measured with enzyme-linked immunosorbent assay. We compared the distribution of genotypes in both groups, evaluated the serum IL-10 level according to the genotypes, and analyzed the association of these polymorphisms with the risk of cervical cancer. The genotype at the -1082 site exhibited only the *A homozygote, and only two haplotypes were found in Korean women, one being -1082*A/-819*T/-592*A (ATA) and the other -1082*A/-819*C/-592*C (ACC). No association was found for IL-10 promoter polymorphisms among the ICC patients in comparison with the NCC subjects. The risk of cervical cancer was not increased in either genotype and the IL-10 serum concentration was not influenced by the genotypes in either group. Polymorphisms of the IL-10 promoter do not appear to be associated with cervical cancer risk or systemic IL-10 production in Korean women.
Roh
Ju Won
JW
Center for Uterine Cancer, National Cancer Center, Goyang, Gyeonggi 411-764, South Korea.
Kim
Moon Hong
MH
Seo
Sang Soo
SS
Kim
Su Hyeong
SH
Kim
Jae Weon
JW
Park
Noh Hyun
NH
Song
Yong Sang
YS
Park
Sang Yoon
SY
Kang
Soon Beom
SB
Lee
Hyo Pyo
HP
D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D006720:Homozygote; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D007723:Korea / Q000453:epidemiology; D009361:Neoplasm Invasiveness / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000235:genetics*; D016387:Women's Health
D005819:Genetic Markers; D016753:Interleukin-10