[Morphological changes in the rat uterine tissues exposed to pregna-D'-pentaran derivatives of progestins and anti-progestins].
Changes in the uterus morphology of mature female rats were studied on the model of pseudopregnancy after treatment with the progestin 16 alpha,17 alpha-cyclohexanoprogesterone (PR) and the antirpogestins 5 alpha(H)-16 alpha,17 alpha-cyclohexano-4,5-dihydroprogesterone (APR1) and 5 beta(H)-16 alpha,17 alpha-cyclohexano-4,5-dihydroprogesterone (APR2). The rats were preliminarily estrogenized with 17 beta-estradiol at a dose of 1 microgram/(animal day) for 4 days and then treated with PR at a dose of 0.2 mg/(animal day) for 14 days. The first group was then left without any treatment, whereas APR1 and APR2 were injected at the dose of 0.2 mg/(animal day) for 4 days to the animals of the second and the third groups, respectively. Light and electron microscopy of the uterus preparations demonstrated that the PR action provoked a complete pseudopregnancy picture characterized by the endometrium functionalization and the myometrium hypertrophy. Subsequent treatment with APR1 and APR2 caused the hypertrophy to cease, which had a more pronounced effect in the case of APR1. At the same time, some indications of the endometrium functionalization remained observable after treatment with APR1 and APR2. The specific binding sites for 3H-labeled APR1 and APR2 were absent from the uterus cytosol for the rats gestagenized with PR.
Kamernitskiĭ
A V
AV
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii pr. 47, GSP-1 Moscow, 117913 Russia. li@ioc.ac.ru
Levina
I S
IS
Kulikova
L E
LE
Milovanov
A P
AP
Khalanskiĭ
A S
AS
Altukhova
V I
VI
Smirnov
A N
AN
Pokrovskaia
E V
EV
Shevchenko
V P
VP
D000818:Animals; D001665:Binding Sites; D003510:Cyclohexanes / Q000737:chemistry / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D011374:Progesterone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D011555:Pseudopregnancy; D051381:Rats; D013237:Stereoisomerism; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
C460860:16,17-cyclohexano-4,5-dihydroprogesterone; D003510:Cyclohexanes; C085261:pentarane A; D011374:Progesterone; D004958:Estradiol