title: The human ASCL2 gene escaping genomic imprinting and its expression pattern.
abstract: The mouse achaete-scute homolog-2 gene (Ascl2 or Mash2) encodes a transcription factor playing a role in the development of the trophoblast. The Ascl2 is an imprinted gene with maternal expression and assigned to an imprinting gene cluster region (ICR) at a distal region of mouse chromosome 7. We previously isolated a phage clone carrying the human homolog, ASCL2, and mapped it to human chromosome 11p15.5, a human ICR. In the present study, we demonstrate the expression patterns of the human ASCL2 in the fetus at a stage between first and second trimesters and in the placental tissues. In addition, it has been shown that the human ASCL2 gene escapes genomic imprinting.
authors.affiliation: Department of Human Genetics, School of Medicine, Nagasaki University, Japan.
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Shiga
authors.initials: S
authors.lastname: Hasuike
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Jinno
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Soejima
authors.forename: Kankatsu
authors.initials: K
authors.lastname: Yun
authors.forename: Kiyonori
authors.initials: K
authors.lastname: Miura
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Norio
authors.initials: N
authors.lastname: Niikawa
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins; D015152:Blotting, Northern; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005314:Embryonic and Fetal Development / Q000235:genetics* / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics* / Q000502:physiology; D018392:Genomic Imprinting / Q000235:genetics* / Q000502:physiology; D006801:Humans; D017403:In Situ Hybridization; D010920:Placenta / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D019105:Random Amplified Polymorphic DNA Technique; D014157:Transcription Factors
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004247:DNA; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C065501:ASCL2 protein, human; C489311:Ascl2 protein, mouse