title: Utility of chromosomal position of heterochromatin as a biomarker of radiation-induced genetic damage: a study of Chornobyl voles (Microtus sp.).
abstract: Biomarkers that effectively document effects of chronic multi-generational exposure to contaminated environments on chromosomes would be valuable in risk assessment, remediation, and environmental decisions. Native, free-ranging populations of voles inhabiting the highly radioactive regions surrounding Reactor 4 of the Chornobyl Nuclear Power Station provide a model system to evaluate biological and chromosomal effects of chronic multi-generational exposure to radioactivity and other reactor meltdown-related pollutants. Here, we explore the utility of heterochromatic elements as potentially informative biomarkers for genetic damage in voles from the radioactive environments surrounding Chornobyl. We analyzed chromosomal positions of heterochromatin from Microtus arvalis and M. rossiaemeridionalis using fluorescent in situ hybridization. Although intrapopulational variation existed in chromosomal position and abundance of heterochromatin, none of that variation could be assigned to environmental exposure.
authors.affiliation: Department of Biological Sciences, Texas Tech University, Lubbock 79409-3131, USA.
authors.forename: Lara E
authors.initials: LE
authors.lastname: Wiggins
authors.forename: Ronald A
authors.initials: RA
authors.lastname: Van Den Bussche
authors.forename: Meredith J
authors.initials: MJ
authors.lastname: Hamilton
authors.forename: Ronald K
authors.initials: RK
authors.lastname: Chesser
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Baker
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000235:genetics*; D015415:Biomarkers; D002869:Chromosome Aberrations; D004249:DNA Damage; D004784:Environmental Monitoring; D006570:Heterochromatin / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D011210:Power Plants; D011832:Radiation Injuries / Q000235:genetics; D018788:Radioactive Hazard Release; D011848:Radioactive Pollutants / Q000009:adverse effects; D018570:Risk Assessment; D014455:Ukraine
chemical_list: D015415:Biomarkers; D006570:Heterochromatin; D011848:Radioactive Pollutants