Biosynthesis of versican by rat dental pulp cells in culture.
The biosynthesis of proteoglycans by these cultured pulp cells was investigated by metabolic labelling, using [(35)S]sulphate, [(3)H]glucosamine and [(3)H]leucine as precursors. Versican-like large proteoglycan, decorin- and biglycan-like small proteoglycans and a small amount of sulphated protein were released into the culture medium. Heparan sulphate species were also identified in cell-layer extracts. Versican-like proteoglycan had an average molecular mass of approximately 800kDa. The molecular mass of chondroihnase ABC-digested core protein exhibited heterogeneity, ranging from 250 to 400kDa, and the glycosaminoglycan chains had an average molecular mass of approximately 42kDa. These results indicate the presence of 10-13 glycosaminoglycan chains per core protein, consistent with the characteristics of versican. This glycosaminoglycan chain contained approximately 63% 4-sulphated disaccharides.
Yoneda
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S
Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. sumieyoneda@aol.com
Shibata
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S
Yamashita
Y
Y
Yanagishita
M
M
D000818:Animals; D058578:Biglycan; D018929:Cell Culture Techniques; D002499:Centrifugation, Density Gradient; D019765:Chondroitin ABC Lyase; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D002809:Chondroitin Sulfates / Q000096:biosynthesis / Q000302:isolation & purification; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D058575:Decorin; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D004187:Disaccharides / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins; D005944:Glucosamine / Q000378:metabolism; D006497:Heparan Sulfate / Q000096:biosynthesis / Q000302:isolation & purification; D037102:Lectins / Q000096:biosynthesis* / Q000302:isolation & purification; D037181:Lectins, C-Type; D007930:Leucine / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D011509:Proteoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D019275:Radiopharmaceuticals; D051381:Rats; D017208:Rats, Wistar; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D014316:Tritium; D053675:Versicans
D058578:Biglycan; D019765:Chondroitin ABC Lyase; D011508:Chondroitin Sulfate Proteoglycans; D002809:Chondroitin Sulfates; D058575:Decorin; D004187:Disaccharides; D016326:Extracellular Matrix Proteins; D005944:Glucosamine; D006497:Heparan Sulfate; D037102:Lectins; D037181:Lectins, C-Type; D007930:Leucine; D011509:Proteoglycans; D019275:Radiopharmaceuticals; D013431:Sulfates; D013462:Sulfur Radioisotopes; D014316:Tritium; D053675:Versicans; C546886:Bgn protein, rat; C546880:Dcn protein, rat; C508324:Vcan protein, rat