title: HPLC measurement of harderoporphyrin in the harderian glands of rodents as a biomarker for sub-lethal or chronic arsenic exposure.
abstract: An improved HPLC method has been established for the measurement of harderoporphyrin (HP) in the harderian gland of rats and mice. Groups of female Wistar rats were given a single oral dose of sodium arsenite at 0, 0.5 or 5.0 mg As(III)/kg body weight, or a slurry of arsenic-contaminated soil at equivalent dose rates and the animals were sacrificed 96 h after dosing. A group of C57BL/6J female mice were chronically exposed to drinking water containing 500 microg As(V)/l of sodium arsenate ad libitum for over 2 years. Porphyrins were measured in the harderian glands of rats and mice. Our results suggest that HP and the alteration of the porphyrin profile in the harderian glands of rodents is a highly sensitive biomarker for both single sub-lethal and chronic arsenic exposure.
authors.affiliation: National Research Centre for Environmental Toxicology, University of Queensland, 39 Kessels Road, Coopers Plains, Brisbane, Qld 4108, Australia. j.ng@mailbox.uq.edu.au
authors.forename: Jack C
authors.initials: JC
authors.lastname: Ng
authors.forename: Lixia
authors.initials: L
authors.lastname: Qi
authors.forename: Michael R
authors.initials: MR
authors.lastname: Moore
mesh_terms: D000818:Animals; D018053:Arsenites / Q000008:administration & dosage / Q000633:toxicity*; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000633:toxicity*; D005260:Female; D006243:Harderian Gland / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011166:Porphyrins / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D017670:Sodium Compounds / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D018053:Arsenites; D015415:Biomarkers; D004791:Enzyme Inhibitors; D011166:Porphyrins; D017670:Sodium Compounds; C040589:harderoporphyrin; C017947:sodium arsenite