Metabolic profiling of rodent biological fluids via 1H NMR spectroscopy using a 1 mm microlitre probe.
The application of a 1 mm TXI (1H/13C/15N) microlitre NMR probe with z-gradient for metabolic profiling of biofluids is described. The probe was used to provide spectral profiles for rat blood plasma using only approximately 2 microl of fluid with a range of solvent suppression techniques. Using a similar amount of fluid, spectra were obtained from rat and mouse cerebrospinal fluid, demonstrating that the probe could be used to profile rodents metabolically via biofluids previously inaccessible to NMR analysis without the need for termination.
Griffin
Julian L
JL
Biological Chemistry, Division of Biomedical Sciences, Faculty of Medicine, Imperial College of Science, Technology and Medicine, South Kensington, London, UK. j.griffin@ic.ac.uk
Nicholls
Andrew W
AW
Keun
Hector C
HC
Mortishire-Smith
Russell J
RJ
Nicholson
Jeremy K
JK
Kuehn
Till
T
D000818:Animals; D001774:Blood Chemical Analysis; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D051379:Mice; D051381:Rats
D006859:Hydrogen