Dye purity and dye standardization for biological staining.
This review starts with a short discussion of what is meant by a pure dye and a brief description of how dyes are produced. A listing of the types of impurities encountered in dyes is made, followed by technical investigations and assessments of dyes and their impurities including methods for separating, identifying and assaying dye components. In the second part of the review, descriptions are given of the standardized staining method approach using standard staining methods for assessing stains, and practical responses to stain impurity including commercial quality control, third-party quality control and standardization of reagents, protocols and documentation. Finally, reference is made to the current state of affairs in the dye field.
Lyon
H O
HO
Department of Pathology, Hvidovre Hospital, University of Copenhagen, Denmark. lyon@post4.tele.dk
D004396:Coloring Agents / Q000302:isolation & purification* / Q000592:standards*; D003718:Denmark; D004340:Drug Contamination; D006651:Histocytochemistry / Q000379:methods* / Q000592:standards* / Q000639:trends; D006801:Humans; D011786:Quality Control; D013194:Staining and Labeling / Q000592:standards*
D004396:Coloring Agents