title: Implication of p53 and caspase-3 in kainic acid but not in N-methyl-D-aspartic acid-induced apoptosis in organotypic hippocampal mouse cultures.
abstract: Apoptotic death is known to be an active process requiring the activation of several apoptotic proteins. Depending on the tissue studied and the stimulus used, these processes are distinct. In this work, we studied if there is a putative implication of the p53 and the caspase-3 proteins in kainic acid (KA) and N-methyl-D-aspartic acid (NMDA)-induced apoptosis in organotypic cultures and if there is any relationship between their respective expressions. We found that KA and NMDA both induce apoptosis but only KA-induced apoptosis is p53- and caspase-3-dependent. This demonstrates that KA and NMDA induce apoptosis following different intracellular pathways.
authors.affiliation: UPR CNRS 9023, CCIPE, Laboratoire de Médecine Expérimentale, Institut de Biologie, Boulevard Henri IV, 34094 Montpellier Cedex 5, France. mdjebai@emory.edu
authors.forename: Myriam
authors.initials: M
authors.lastname: Djebaïli
authors.forename: Frédéric
authors.initials: F
authors.lastname: De Bock
authors.forename: Valérie
authors.initials: V
authors.lastname: Baille
authors.forename: Joël
authors.initials: J
authors.lastname: Bockaert
authors.forename: Gérard
authors.initials: G
authors.lastname: Rondouin
mesh_terms: D000818:Animals; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D053938:DNA Fragmentation / Q000187:drug effects / Q000502:physiology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology*; D007608:Kainic Acid / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000166:cytology* / Q000201:enzymology; D009498:Neurotoxins / Q000494:pharmacology; D009924:Organ Culture Techniques; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D018690:Excitatory Amino Acid Agonists; D009498:Neurotoxins; D016159:Tumor Suppressor Protein p53; D016202:N-Methylaspartate; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases; D007608:Kainic Acid