title: Mucin expression in the ileoanal reservoir reflects incomplete mucosal adaptation.
abstract: Restorative proctocolectomy is regarded as a standard surgical procedure for patients who require a proctocolectomy for ulcerative colitis and familial adenomatous polyposis. The ileal mucosa undergoes colonic phenotypic change with time, but the extent and relevance of these changes to the long-term safety of the ileoanal pouch are unclear. The aim of this study was to study the mucin biology of this adaptive process in order to assess its extent and possible impact on pouch safety. Ileoanal pouch biopsies from a cohort of patients and normal ileal and colonic controls were subjected to histological, biochemical, histochemical, and immunohistochemical mucin analysis. Mucin sulphation and sialic acid O-acetylation were studied as parameters of colonic phenotypic change. Fifty-one patients, 16 ileal, and 22 colonic controls were studied. Seventy per cent of biopsies retained villous mucosal architecture, with no cases of dysplasia detected. Ileoanal pouch mucosal sulphation and sialic acid O-acetylation did not reach colonic levels, thus indicating limited evidence for a more colonic phenotype. The data from this study suggest that colonic phenotypic change within the ileoanal reservoir is incomplete, with no cases of dysplasia detected. The degree of phenotypic change is less than in previous studies, which may support, but not prove, our hypothesis that there may be a process of reversion to an ileal type mucosa in the ileoanal reservoir with time.
authors.affiliation: Department of Surgery, Bristol Royal Infirmary, UK. sylvesterpa@hotmail.com
authors.forename: Paul A
authors.initials: PA
authors.lastname: Sylvester
authors.forename: Newton A C S
authors.initials: NA
authors.lastname: Wong
authors.forename: Neil
authors.initials: N
authors.lastname: Myerscough
authors.forename: Bryan F
authors.initials: BF
authors.lastname: Warren
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Corfield
authors.forename: Michael G
authors.initials: MG
authors.lastname: Thomas
authors.forename: Paul
authors.initials: P
authors.lastname: Durdey
mesh_terms: D000222:Adaptation, Physiological; D001706:Biopsy; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007082:Ileum / Q000378:metabolism* / Q000503:physiopathology; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000503:physiopathology; D009077:Mucins / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009924:Organ Culture Techniques; D011184:Postoperative Period; D016737:Proctocolectomy, Restorative
chemical_list: D006023:Glycoproteins; D009077:Mucins; D019158:N-Acetylneuraminic Acid