Effects of human antithymocyte globulin on acetylcholine synthesis, its release and choline acetyltransferase transcription in a human leukemic T-cell line.
Lymphocytes possess an independent, nonneuronal cholinergic system. In the present study, we investigated the short- and long-term effects of antithymocyte globulin (ATG)-Fresenius (ATG-F), a human antithymocyte globulin that binds to CD2, CD7 and CD11a, on acetylcholine (ACh) synthesis and transcription of choline acetyltransferase (ChAT) in CCRF-CEM cells, a human leukemic T-cell line. In the short-term (6 h), ATG-F enhanced ACh release, likely through transient increases in intracellular Ca(2+) ([Ca(2+)](i)) mediated by CD7, which led to declines in intracellular ACh content. By 48 h, however, the ACh content had increased as compared to control due to up-regulation of ChAT expression mediated by CD11a.
Fujii
Takeshi
T
Department of Pharmacology, Kyoritsu College of Pharmacy, 1-5-30 Shibakoen, Minato, Tokyo 105-8512, Japan.
Ushiyama
Naoki
N
Hosonuma
Kazuyo
K
Suenaga
Aya
A
Kawashima
Koichiro
K
D000109:Acetylcholine / Q000096:biosynthesis* / Q000378:metabolism; D019011:Antigens, CD7 / Q000276:immunology / Q000378:metabolism; D000954:Antigens, Surface / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D000961:Antilymphocyte Serum / Q000276:immunology* / Q000494:pharmacology; D018845:CD11 Antigens / Q000276:immunology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects / Q000276:immunology; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D002795:Choline O-Acetyltransferase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007424:Intracellular Fluid / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D007938:Leukemia; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects / Q000276:immunology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology
D019011:Antigens, CD7; D000954:Antigens, Surface; D000961:Antilymphocyte Serum; D018845:CD11 Antigens; D012333:RNA, Messenger; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine; D002118:Calcium