Identification of autoantibodies associated with systemic lupus erythematosus.
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the presence of antinuclear antibodies. We performed serological analysis of cDNA expression library (SEREX) to identify autoantibodies associated with SLE. The screening of three different cDNA expression libraries with pooled sera of patients with SLE yielded 11 independent clones that reacted with pooled sera of patients with SLE. In this screening, autoantibodies to poly(ADP-ribose) polymerase (PARP), U1snRNP, and galectin-3 were prevalent in the sera of patients with SLE (26/68, 25/68, 12/63, respectively). The frequency of autoantibody to PARP was significantly higher in SLE than that of healthy donors (0/76) (38.2% vs 0%, p<0.00001). The autoantibody to PARP was infrequently detected in the serum of patients with RA (1/50). However, autoantibody to PARP was not found in the sera of patients with other rheumatic diseases including Sjogren's syndrome (0/19), systemic sclerosis (0/18), and polymyositis/myositis (0/37). The frequency of autoantibody to human galectin-3 (12/63) was significantly higher in SLE than that of healthy donors (0/56) (19% vs 0%, p=0.0006). Autoantibody to galectin-3 was not found in the sera of patients with rheumatoid arthritis (0/50), Sjogren's syndrome (0/18), and systemic sclerosis (0/19). Interestingly, autoantibody to galectin-3 was also prevalent in the sera of patients with polymyositis/dermatomyositis (16/37, 43.2%). Further functional characterization of these autoantibodies would be necessary to determine their value as diagnostic markers or to define clinical subsets of patients with SLE. Statistical analysis revealed that the presence of autoantibody to PARP was inversely related with pleurisy, and the presence of autoantibody to galectin-3 related with renal disease.
Lim
Yoon
Y
Cancer Genomics Division, In2Gen Company, 6th Floor, Cancer Research Center, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea.
Lee
Dae-Yeon
DY
Lee
Seongeun
S
Park
Sae-Young
SY
Kim
Jongwan
J
Cho
Bomsoo
B
Lee
Hosoon
H
Kim
Hae-Yeong
HY
Lee
Eunbong
E
Song
Yeong Wook
YW
Jeoung
Doo-Il
DI
D000328:Adult; D000943:Antigens, Differentiation / Q000276:immunology; D001323:Autoantibodies / Q000097:blood / Q000235:genetics / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology; D005260:Female; D037502:Galectin 3; D015723:Gene Library; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000276:immunology*; D008297:Male; D011065:Poly(ADP-ribose) Polymerases / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000276:immunology
D000943:Antigens, Differentiation; D001323:Autoantibodies; D001324:Autoantigens; D037502:Galectin 3; D012333:RNA, Messenger; D017412:Ribonucleoprotein, U1 Small Nuclear; D011065:Poly(ADP-ribose) Polymerases