Electrochemical analysis of sparfloxacin in pharmaceutical formulation and biochemical screening of its Co(II) complex.
Simple and sensitive, direct current polarographic (DCP) and differential pulse polarographic (DPP) methods have been developed for the qualitative as well as quantitative analysis of 5-amino-1-cyclopropyl-7 (cis-3,5 dimethyl-1-piperazinyl)-6,8-difluoro-1,4-dihydro-4-oxo-3-quinoline carboxylic acid (sparfloxacin). The developed methods have been standardized for the determination of the drug in pharmaceutical formulation for quality control purposes. The observed data has been subjected to statistical analysis, which revealed high reliability and precision. Sparfloxacin forms a complex with Co(II) which has been characterized on the basis of elemental analysis, IR spectral, polarographic and amperometric analysis. The analytical results indicated a 1:1 (M:L) stoichiometry for the Co(II)-sparfloxacin complex. The antibacterial studies on the drug and its complex were carried out against various pathogenic bacteria. The results revealed that the complex is more potent compared to the pure drug.
Jain
Swati
S
Department of Chemistry, Dr. H.S. Gour University, (M.P.) 470 003, Sagar, India.
Jain
N K
NK
Pitre
K S
KS
D000890:Anti-Infective Agents / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D003035:Cobalt / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D024841:Fluoroquinolones; D008826:Microbial Sensitivity Tests; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D004364:Pharmaceutical Preparations; D003035:Cobalt; C061363:sparfloxacin