Modulation by nutrients and drugs of liver acyl-CoAs analyzed by mass spectrometry.
The profile of liver acyl-CoAs induced by dietary fats of variable compositions or by xenobiotic hypolipidemic amphipathic carboxylates was evaluated in vivo using a novel electrospray ionization tandem mass spectrometry methodology of high resolution, sensitivity, and reliability. The composition of liver fatty acyl-CoAs was found to reflect the composition of dietary fat. Treatment with hypolipidemic carboxylates resulted in liver dominant abundance of their respective acyl-CoAs accompanied by an increase in liver fatty acyl-CoAs. Cellular effects exerted by dietary fatty acids and/or xenobiotic carboxylic drugs may be transduced in vivo by their respective acyl-CoAs.
Kalderon
B
B
Department of Human Nutrition and Metabolism, Hebrew University Medical School, Jerusalem, Israel 91120.
Sheena
V
V
Shachrur
S
S
Hertz
R
R
Bar-Tana
J
J
D000214:Acyl Coenzyme A / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001629:Bezafibrate / Q000494:pharmacology; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000494:pharmacology; D008099:Liver / Q000737:chemistry* / Q000187:drug effects*; D008297:Male; D009255:Nafenopin / Q000494:pharmacology; D010169:Palmitic Acids / Q000494:pharmacology; D051381:Rats; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D015262:Xenobiotics / Q000494:pharmacology*
D000214:Acyl Coenzyme A; D004041:Dietary Fats; D000960:Hypolipidemic Agents; D010169:Palmitic Acids; D015262:Xenobiotics; D009255:Nafenopin; C056166:MEDICA 16; D001629:Bezafibrate