Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer.
Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.
van de Weert
Marco
M
Department of Pharmaceutics, Faculty of Pharmacy, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht, The Netherlands. mvdw@dfh.dk
van Dijkhuizen-Radersma
Riemke
R
Bezemer
Jeroen M
JM
Hennink
Wim E
WE
Crommelin
Daan J A
DJ
D001672:Biocompatible Materials; D009113:Muramidase / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
D001672:Biocompatible Materials; D011091:Polyesters; D011093:Polyethylene Terephthalates; D013501:Surface-Active Agents; C041733:poly(1,4-butylene terephthalate); D009113:Muramidase