Tryptophan decarboxylase from transformed roots of Catharanthus roseus.
Tryptophan decarboxylase (TDC, EC 4.1.1.28) from Catharanthus roseus hairy roots was purified 80-fold. Antibodies against TDC were obtained and they recognized only one protein of 55 kDa in crude extracts from hairy root cultures. Elicitation of transformed root cultures with macerozyme yielded a marked increase in TDC activity, which was accompanied by a similar increase in the amount of immunoreactive TDC protein. These results suggest that the alkaloid accumulation, produced by elicitation, requires the synthesis of new TDC polypeptide in C. roseus root cultures and establishes important differences in the regulatory control of this enzyme in root cultures compared to developing seedlings, where the posttranslational regulation apparently plays a major role.
Islas-Flores
Ignacio
I
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigacíon Científica de Yucatán, México.
Moreno-Valenzuela
Oscar
O
Minero-García
Yereni
Y
Loyola-Vargas
Victor M
VM
Miranda-Ham
María de Lourdes
Mde L
D000470:Alkaloids / Q000378:metabolism; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D030008:Catharanthus / Q000201:enzymology* / Q000235:genetics; D046508:Culture Techniques; D015151:Immunoblotting; D018517:Plant Roots / Q000201:enzymology* / Q000235:genetics; D030821:Plants, Genetically Modified
D000470:Alkaloids; D001142:Aromatic-L-Amino-Acid Decarboxylases