title: Enhanced rate of nerve regeneration and directional errors after sciatic nerve injury in receptor protein tyrosine phosphatase sigma knock-out mice.
abstract: The receptor protein tyrosine phosphatase sigma (PTPsigma) is a member of the mammalian leukocyte common antigen-related (LAR) family. Its expression is developmentally regulated in neuronal tissues. The Drosophila homolog of the mammalian LAR family of phosphatases (DLAR) controls axon guidance during Drosophila embryogenesis. We have demonstrated previously that mice deficient in PTPsigma have CNS and peripheral nervous system abnormalities. The sciatic nerve in the PTPsigma(-/-) mice demonstrates an increased number of small diameter fibers and slower nerve conduction velocities compared with PTPsigma(+/+) or PTPsigma(+/-) controls. To study whether peripheral nerve regeneration is affected by PTPsigma activity, we assessed nerve regeneration in the PTPsigma(-/-) mouse after three standard models of sciatic nerve injury. We report that after sciatic nerve crush injury, nerve regeneration was significantly faster in the PTPsigma(-/-) animals, as determined by histologic, electrophysiologic, and neuromuscular testing. After sciatic nerve transection with immediate microsurgical repair or allografting, PTPsigma(-/-) nerve fibers demonstrated errors in directional growth compared with controls. We propose that PTPsigma regulates the axonal regeneration rate and guidance of regenerating fibers.
authors.affiliation: Department of Pediatrics, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada, M5G 1X8.
authors.forename: Joanna
authors.initials: J
authors.lastname: McLean
authors.forename: Jane
authors.initials: J
authors.lastname: Batt
authors.forename: Laurie C
authors.initials: LC
authors.lastname: Doering
authors.forename: Daniela
authors.initials: D
authors.lastname: Rotin
authors.forename: James R
authors.initials: JR
authors.lastname: Bain
mesh_terms: D000200:Action Potentials; D000818:Animals; D001369:Axons / Q000502:physiology; D001522:Behavior, Animal; D007700:Kinetics; D051379:Mice; D018345:Mice, Knockout; D009043:Motor Activity; D009068:Movement; D009409:Nerve Crush; D009412:Nerve Fibers / Q000502:physiology / Q000648:ultrastructure; D009416:Nerve Regeneration; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000502:physiology*; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2; D012584:Sciatic Nerve / Q000166:cytology / Q000502:physiology* / Q000601:surgery / Q000637:transplantation
chemical_list: D017027:Protein Tyrosine Phosphatases; C516885:Ptprs protein, mouse; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2