title: [Study on the prevention of insulitis and diabetes by complete Freund's adjuvant in NOD female mice].
abstract: The degrees of insulitis, the incidence of diabetes, and the counts of alpha,beta and delta cells in islets were studied by morphological method and avidin-biotin complex immunohistochemical method in complete Freund's adjuvant(CFA) or normal saline(NS)-treated non-obese diabetic(NOD) female mice. The results showed: The incidences of in-insulitis and the scoring means were significantly lower in CFA-treated mice than those in NS-treated ones; none of 5 CFA-treated mice and 3 out of 5 NS-treated ones developed diabetes. The positive rates of alpha, and delta cells were significantly lower in CFA-treated mice than those in NS-treated ones, but the positive rate of beta cell was significantly higher in CFA-treated mice than that in NS-treated ones. The correlations were found among the scores of insulitis and the positive rates of alpha, beta, delta cells in islets (r alpha = 0.475, r beta = -0.878, r delta = 0.869). The results indicate that CFA may lessen the degrees of insulitis and the incidence of diabetes. Its effects might be related to lessen the damage of beta cell in islets.
authors.affiliation: Research Laboratory of Hepatobiliary Diseases, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
authors.forename: Z
authors.initials: Z
authors.lastname: Yang
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: Z
authors.initials: Z
authors.lastname: Zhou
authors.forename: E Y
authors.initials: EY
authors.lastname: Liao
authors.forename: W
authors.initials: W
authors.lastname: Yang
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000517:prevention & control*; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000473:pathology*; D051379:Mice; D016688:Mice, Inbred NOD; D010195:Pancreatitis / Q000188:drug therapy; D011897:Random Allocation
chemical_list: D005620:Freund's Adjuvant