The effect of N-acetyl-L-cysteine on the viscosity of ileal neobladder mucus.
N-acetyl-L-cysteine (NAC) proved to be an effective mucolytic in pulmonary secretions. Our goal was to investigate the in vitro effect of NAC on viscosity of ileal neobladder mucus. The urine of a patient with an ileal neobladder was collected during the first 7 days postoperatively and stored in a refrigerator. After precipitation, the urine was decanted. The residue was stirred to a homogeneous suspension. To samples of 4.5 ml mucus, 0.5 ml NAC 10% was added. To the control sample, 0.5 ml water was added. The samples were incubated in a water bath at 37 degrees C for 5, 30 and 60 min. Viscosity was measured in the Bohlin VOR Rheometer. The viscosity of the ileal neobladder mucus decreased quickly after incubating with NAC 10%. Viscosity increased slightly after I h of incubation. The viscosity in the control sample was higher than in the other incubated samples. NAC was found to decrease the viscosity of ileal neobladder mucus, supporting the in vivo experience that NAC can be useful in patients with an ileal neobladder to facilitate the evacuation of mucus by decreasing viscosity.
Schrier
B P
BP
University Medical Centre, St Radboud, Nijmegen, The Netherlands. b.schrier@uro.azn.nl
Lichtendonk
W J
WJ
Witjes
J A
JA
D000111:Acetylcysteine / Q000627:therapeutic use*; D005100:Expectorants / Q000627:therapeutic use*; D006801:Humans; D007082:Ileum / Q000378:metabolism* / Q000601:surgery*; D009093:Mucus / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D019738:Surgically-Created Structures; D001743:Urinary Bladder / Q000601:surgery*; D014556:Urine / Q000737:chemistry; D014783:Viscosity
D005100:Expectorants; D000111:Acetylcysteine