Suppression and acceleration of cell elongation by integration of xyloglucans in pea stem segments.
Xyloglucan is a key polymer in the walls of growing plant cells. Using split pea stem segments and stem segments from which the epidermis had been peeled off, we demonstrate that the integration of xyloglucan mediated by the action of wall-bound xyloglucan endotransglycosylase suppressed cell elongation, whereas that of its fragment oligosaccharide accelerated it. Whole xyloglucan was incorporated into the cell wall and induced the rearrangement of cortical microtubules from transverse to longitudinal; in contrast, the oligosaccharide solubilized xyloglucan from the cell wall and maintained the microtubules in a transverse orientation. This paper proposes that xyloglucan metabolism controls the elongation of plant cells.
Takeda
Takumi
T
Wood Research Institute, Kyoto University, Gokasho, Uji 611-0011, Japan.
Furuta
Yuzo
Y
Awano
Tatsuya
T
Mizuno
Koichi
K
Mitsuishi
Yasushi
Y
Hayashi
Takahisa
T
D002473:Cell Wall / Q000378:metabolism; D005936:Glucans; D006031:Glycosylation; D008870:Microtubules; D018532:Pisum sativum / Q000166:cytology* / Q000378:metabolism; D018547:Plant Stems / Q000166:cytology / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D014990:Xylans
D005936:Glucans; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan