title: Molecular cloning and characterization of oppo 1: a haploid germ cell-specific complementary DNA encoding sperm tail protein.
abstract: We isolated a cDNA clone specifically expressed during spermatogenesis from a subtracted cDNA library of mouse testis. The cDNA consisted of 1085 nucleotides and had an open reading frame of 870 nucleotides encoding a putative protein of 290 amino acid residues. Northern blot analysis revealed a 1.2-kilobase mRNA exclusively expressed in the testis in adult mice; the mRNA was first detected late pachytene stage, and expression increased as the animals matured. The protein encoded by the mRNA had a molecular weight of approximately 33 kDa by Western blot analysis, and was localized to occupy the flagella from the connecting piece through the principal piece. We named this newly isolated gene oppo 1, and we suggest that it plays an important role in sperm tail structure and/or sperm movement.
authors.affiliation: Department of Urology, Osaka University Medical School, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Tanaka
authors.forename: Minoru
authors.initials: M
authors.lastname: Koga
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Miyagawa
authors.forename: Naoko
authors.initials: N
authors.lastname: Iguchi
authors.forename: Carlos
authors.initials: C
authors.lastname: Egydio de Carvalho
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yomogida
authors.forename: Masami
authors.initials: M
authors.lastname: Nozaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nojima
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Matsumiya
authors.forename: Akihiko
authors.initials: A
authors.lastname: Okuyama
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Nishimune
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D015723:Gene Library; D006238:Haploidy; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D029589:Seminal Plasma Proteins / Q000235:genetics; D013081:Sperm Motility / Q000235:genetics; D013082:Sperm Tail / Q000378:metabolism*; D013347:Subcellular Fractions / Q000737:chemistry; D013737:Testis / Q000737:chemistry
chemical_list: D012333:RNA, Messenger; D029589:Seminal Plasma Proteins; C470246:oppo 1 protein, mouse