title: Immortalisation of ovarian granulosa and Theca cells of the marmoset monkey Calllithrix jacchus.
abstract: In view of the increasing need for laboratory primates in biomedical research it is desirable to develop appropriate primate-specific cell culture models that could prevent or significantly reduce the increasing use of primary cultures and experiments with living animals. Follicular granulosa and theca cells are essential for the control of hormone-dependent processes such as the ovarian cycle and pregnancy, but also for the occurrence of hormone-dependent diseases. For this reason it is of great interest to know more about control mechanisms existing in these follicular cell types and the effect of pharmacological or toxicological agents on them. An immortalisation protocol for the two ovarian cell types of the marmoset monkey (Callithrix jacchus) has been developed. All cell lines established so far were examined with regard to the maintenance of known, tissue-specific features (e.g. hormone responsiveness and enzyme expression). The results obtained indicate that it is worth while cloning and characterising the cell lines in more detail so that they could be used after an adequate validation as a defined test system both for basic research as well as for pharmacological or toxicological screening.
authors.affiliation: Deutsches Primatenzentrum, D-Göttingen, Germany. bhusen@www.dpz.gwdg.de
authors.forename: Bettina
authors.initials: B
authors.lastname: Husen
authors.forename: Kai
authors.initials: K
authors.lastname: Lieder
authors.forename: Alexandra
authors.initials: A
authors.lastname: Marten
authors.forename: Angelika
authors.initials: A
authors.lastname: Jurdzinski
authors.forename: Kerstin
authors.initials: K
authors.lastname: Fuhrmann
authors.forename: Harald
authors.initials: H
authors.lastname: Petry
authors.forename: Alois
authors.initials: A
authors.lastname: Lüke
authors.forename: Almuth
authors.initials: A
authors.lastname: Einspanier
mesh_terms: D000826:Animal Testing Alternatives; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis / Q000235:genetics; D002144:Callithrix; D018929:Cell Culture Techniques / Q000379:methods; D002461:Cell Line, Transformed; D005260:Female; D016679:Genome, Viral; D006107:Granulosa Cells / Q000166:cytology*; D006080:Ovarian Follicle / Q000166:cytology; D011994:Recombinant Proteins / Q000032:analysis; D013539:Simian virus 40 / Q000235:genetics; D013799:Theca Cells / Q000166:cytology*; D014162:Transfection
chemical_list: D000952:Antigens, Polyomavirus Transforming; D011994:Recombinant Proteins