Isolation, characterization and mapping of the mouse and human RB1CC1 genes.
RB1CC1 (RB1-inducible Coiled-Coil 1), a putative transcription factor implicated in the regulation of RB1 (retinoblastoma 1) expression, was recently identified in a screen for genes involved in multi-drug resistance to anticancer agents. Information about the RB1CC1 gene is limited, however, and its biological function is not determined. Here we report the isolation, characterization and mapping of the mouse RB1CC1 gene (Rb1cc1), together with further characterization of the human RB1CC1 gene. Mouse Rb1cc1 encodes 1588 amino acids, sharing 89% identity and key sequence motifs with its human counterpart. Rb1cc1 is expressed abundantly in heart and testis, with lower levels detected in lung and spleen. Immunohistochemical analysis revealed the Rb1cc1 and Rb1 proteins are co-localized in the cell nuclei of NIH3T3-3 cell and various mouse tissues. The human and mouse RB1CC1 genes, both of which contain 24 exons, span 74 kb on chromosome 8q11.2 and 57 kb on chromosome 1A2-4, respectively. Conserved sequence motifs and nuclear localization suggest that the RB1CC1 proteins function as transcription factors.
Chano
Tokuhiro
T
Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Tsukinowa-cho, Seta, Otsu, Shiga 520-2192, Japan. chano@belle.shiga-med.ac.jp
Ikegawa
Shiro
S
Saito-Ohara
Fumiko
F
Inazawa
Johji
J
Mabuchi
Akihiko
A
Saeki
Yukikazu
Y
Okabe
Hidetoshi
H
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000071183:Autophagy-Related Proteins; D002467:Cell Nucleus / Q000235:genetics; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D005091:Exons; D015870:Gene Expression; D005796:Genes / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases; D016160:Retinoblastoma Protein / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D026801:Synteny
D000071183:Autophagy-Related Proteins; D018076:DNA, Complementary; C407704:RB1CC1 protein, human; D016160:Retinoblastoma Protein; D011505:Protein-Tyrosine Kinases