Evaluation of PCR primers and PCR conditions for specific detection of common airborne fungi.
We examined the selectivity of 53 sets of primers for environmental monitoring of indoor air quality. Thirty-six fungal strains, representing 26 species from 14 genera of commonly occurring fungi, and 16 different bacterial strains, representing both gram-negative and gram-positive species, were included in the experiment. We verified the specificity of 28 of the 53 sets of primers, which were classified as universal fungal, universal bacterial, group or species specific. The PCR conditions required for optimal specificity were also determined. These results can serve as a guide for the step-wise PCR-based detection and identification of airborne fungi commonly found in indoor environments.
Wu
Zhihong
Z
Department of Chemistry, The National Institute for Working Life, Umeå, Sweden.
Wang
Xiao-Ru
XR
Blomquist
Göran
G
D016902:Air Pollution, Indoor / Q000032:analysis*; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D005658:Fungi; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004271:DNA, Fungal