Effect of myocardial stunning on thiol status, myofibrillar ATPase and troponin I proteolysis.
To investigate the mechanism underlying postischemic contractile dysfunction (myocardial stunning) we examined myocardial sulflhydryl group content, myofibrillar Ca2+-dependent Mg2+-ATPase activity and protein profile after global ischemia and reperfusion. The Langerdorff-perfused rabbit hearts were subjected to 15 min normothermic ischemia followed by 10 min reperfusion and myofibrils were isolated from homogenates of left ventricular tissues. Depressed contractile function during reperfusion was accompanied by a decrease in total sulfhydryl group content. However, myofibrillar protein profile was unchanged and Western immunoblotting analysis showed no significant differences in troponin I immunoreactive bands between control and stunned hearts. Likewise, myofibrillar Mg2+-ATPase activity was unaltered after ischemia and reperfusion. We conclude that myocardial stunning is not caused by altered myofibrillar function and protein degradation but may be partly due to the oxidative modification of as yet undefined proteins.
Kaplan
Peter
P
Centre for Experimental Surgery and Anaesthesiology, Katholieke Universiteit, Leuven, Belgium. kaplan@jfmed.uniba.sk
Matejovicová
Milena
M
Lehotsky
Ján
J
Flameng
Willem
W
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D003546:Cysteine Endopeptidases / Q000494:pharmacology; D009097:Multienzyme Complexes / Q000494:pharmacology; D009200:Myocardial Contraction / Q000502:physiology; D015428:Myocardial Reperfusion Injury / Q000201:enzymology / Q000503:physiopathology; D017682:Myocardial Stunning / Q000201:enzymology* / Q000503:physiopathology; D009210:Myofibrils / Q000201:enzymology*; D046988:Proteasome Endopeptidase Complex; D011817:Rabbits; D013438:Sulfhydryl Compounds; D019210:Troponin I / Q000378:metabolism*
D009097:Multienzyme Complexes; D013438:Sulfhydryl Compounds; D019210:Troponin I; D000255:Adenosine Triphosphate; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D017301:Ca(2+) Mg(2+)-ATPase; D002118:Calcium