Gene structure of the carp fish ribosomal protein L41: seasonally regulated expression.
The seasonal acclimatization of the carp fish demands physiological compensatory responses. The process involves profound nucleolar adjustments and remarkable changes in rRNA synthesis, which affect ribosomal biogenesis. We have documented that protein kinase CK2, whose activity is related to ribosomal protein L41 and the regulation of rRNA synthesis, was expressed in notably higher amounts in summer-acclimatized carp compared to the cold-season adapted fish. Thus, we approached the study of the functional genomics of carp L41 protein. We report the first cloning of a fish L41 gene encoding the highly conserved 25 amino acids, including approximately 1700 bp regulatory upstream region and the 3(') polyadenylation signal, plus the isolation and characterization of two different L41 cDNAs. We found a clear differential expression of L41, which follows the same pattern as protein kinase CK2beta that transcribes at higher levels in the summer-acclimatized carp than it does in the winter-adapted fish.
Molina
Alfredo
A
Facultad de Ciencias de la Salud, Millennium Institute for Fundamental and Applied Biology, Universidad Andrés Bello, República 217, 4to piso, Santiago, Chile.
Corta
Aintzane
A
Martin
Rody San
RS
Alvarez
Marco
M
Burzio
Luis O
LO
Krauskopf
Manuel
M
Vera
María Inés
MI
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002347:Carps; D047390:Casein Kinase II; D018076:DNA, Complementary / Q000378:metabolism; D005786:Gene Expression Regulation; D015723:Gene Library; D008297:Male; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism*; D012621:Seasons; D014018:Tissue Distribution
D018076:DNA, Complementary; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases