Development of a biocatalytic process for the production of c6-aldehydes from vegetable oils by soybean lipoxygenase and recombinant hydroperoxide lyase.
Volatile C6- and C9-aldehydes and alcohols are widely used as food flavors to reconstitute the "fresh green" odor of fruits and vegetables lost during processing. To meet the high demand for natural flavors, an efficient, cheap, and versatile biocatalytic process was developed to produce C6-aldehydes on a large scale. Vegetable oils were converted by soybean lipoxygenase and recombinant hydroperoxide lyase into hexanal and (2E)- or (3Z)-hexenal. In contrast to plant extracts, generally used as enzyme sources, high molar conversions were obtained with recombinant hydroperoxide lyase (50% for hexanal and 26% for hexenal formation), and no side products were formed. Furthermore, recombinant hydroperoxide lyase lacks isomerase activity, allowing production of (3Z)-hexenal, which could not be obtained in previously described processes. Recombinant hydroperoxide lyase is stable and can be stored at 4 degrees C for 1 month without significant loss of activity.
Noordermeer
Minke A
MA
Bijvoet Center for Biomolecular Research, Department of Bio-organic Chemistry, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Van Der Goot
Wouter
W
Van Kooij
Aukje J
AJ
Veldsink
Jan Willem
JW
Veldink
Gerrit A
GA
Vliegenthart
Johannes F G
JF
D000446:Aldehyde-Lyases / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism*; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006591:Hexobarbital / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008084:Lipoxygenase / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013025:Glycine max / Q000201:enzymology*
D000447:Aldehydes; D010938:Plant Oils; D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System; C010463:n-hexanal; D006591:Hexobarbital; D008084:Lipoxygenase; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase