title: Analysis of T-cell repertoire and mixed chimaerism in a patient with aplastic anaemia after allogeneic bone marrow transplantation.
abstract: We analysed 26 T-cell receptor (TCR) beta chain subfamilies (VB) of a patient with aplastic anaemia (AA) who underwent allogeneic bone marrow transplantation (allo-BMT). The patient developed pancytopenia at d 80. The patient's T cells were skewed in 10 of 26 TCR-VB on d 83. These TCR-VB, especially VB15, which were almost entirely CD8-positive cells, were skewed throughout her clinical course. Chimaerism analysis of the CD8-positive cells indicated that they were of recipient origin. Therefore, some immune responses induced by the recipient CD8-positive T cells had an important role in pancytopenia in AA patients after allo-BMT.
authors.affiliation: Department ofHaematology and Oncology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tsutsumi
authors.forename: Junji
authors.initials: J
authors.lastname: Tanaka
authors.forename: Junichi
authors.initials: J
authors.lastname: Sugita
authors.forename: Naoko
authors.initials: N
authors.lastname: Kato
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Yonezumi
authors.forename: Kouji
authors.initials: K
authors.lastname: Chiba
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Toyosima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kondo
authors.forename: Shuichi
authors.initials: S
authors.lastname: Ohta
authors.forename: Akio
authors.initials: A
authors.lastname: Mori
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hasino
authors.forename: Masahiro
authors.initials: M
authors.lastname: Asaka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Imamura
mesh_terms: D000328:Adult; D000741:Anemia, Aplastic / Q000276:immunology* / Q000628:therapy*; D016026:Bone Marrow Transplantation; D019075:Electrophoresis, Capillary; D005260:Female; D019674:Genes, T-Cell Receptor beta; D006801:Humans; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous