Crystallization and preliminary analysis of Escherichia coli YodA.
The Escherichia coli protein YodA was overexpressed, purified and crystallized in several crystal forms. The function of this protein is not known, although it has been identified under conditions of bacterial stress. Three of the four crystal forms were obtained in the presence of divalent cations (zinc, nickel and cadmium), suggesting that YodA may be a metal-binding protein.
David
Gabriel
G
LURE, CNRS, CEA, MR, BP 34, 91898 Orsay CEDEX, France.
Blondeau
Karine
K
Renouard
Madalena
M
Lewit-Bentley
Anita
A
D002104:Cadmium / Q000737:chemistry; D018360:Crystallography, X-Ray / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015032:Zinc / Q000737:chemistry
D029968:Escherichia coli Proteins; C470844:ZinT protein, E coli; D002104:Cadmium; D009532:Nickel; D015032:Zinc