Human placental lipid induces mitogenesis and melanogenesis in B16F10 melanoma cells.
A hydroalcoholic extract of fresh term human placenta was found to be mitogenic as well as melanogenic on B16F10 mouse melanoma in an in vitro culture. The extract, a reservoir of a large number of bioactive molecules, was resolved to get the lipid fraction. Its activity was evaluated on B16F10 mouse melanoma by assessing the change in cellular morphology, growth and melanin induction. The lipid fraction, placental total lipid fraction (PTLF) tested in the study employed doses of 0 01 to 200 microg/ml; optimum growth and melanization accompanied by morphological changes were recorded at 10 and 100 microg/ml respectively. At intermediate doses growth and melanization were found to show a pattern of change over between growth and melanization and finally reached at an inverse relation at the respective optimal dose of response. Compared with defined sphingolipids, C(2) ceramide and sphingosine-1-phosphate, the results were mostly corroborative. The duality of biological response of sphingolipids as reported in numerous studies was comparable for the PTLF suggesting that its active component is a sphingolipid and showing its use for pigment recovery in vitiligo.
Mallick
Shampa
S
Department of Cellular Biochemistry, Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Jadavpur, Kolkata 700 032, India.
Mandal
Samir Kumar
SK
Bhadra
Ranjan
R
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008055:Lipids / Q000494:pharmacology*; D008246:Lysophospholipids; D008543:Melanins / Q000096:biosynthesis*; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D010924:Placental Extracts / Q000737:chemistry*; D013107:Sphingolipids / Q000494:pharmacology; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D008055:Lipids; D008246:Lysophospholipids; D008543:Melanins; C064769:N-acetylsphingosine; D010924:Placental Extracts; D013107:Sphingolipids; C060506:sphingosine 1-phosphate; D013110:Sphingosine