Effect of simple phenolic compounds on azoxymethane-induced aberrant crypt foci in rat colon.
Because complex mixtures of plant polyphenols exert anticancer activity in animal models, we investigated whether low-molecular-weight natural phenolic compounds (2-OH-coumaric acid, 3-OH-coumaric acid, 4-OH-coumaric acid, 3-OH-flavone, 7-OH-flavone, 4-OH-benzoic acid, 3-OH-benzoic acid, and 2,3-OH-benzoic acid) affect azoxymethane (AOM)-induced aberrant crypt foci (ACF), which have been suggested to represent preneoplastic lesions, in the colon of rats. Male Fischer 344 rats were fed diets supplemented with 0.1% (wt/wt) of the different phenolic compounds, and after 2 wk they were treated twice (1 wk apart) with AOM (15 mg/kg s.c.); the dietary treatment continued until sacrifice, 7 wk after the first injection with AOM. The results showed that none of these phenolic compounds exerted chemopreventive activity on the ACF assay. On the contrary, 3-OH-flavone slightly, although significantly, increased (P < 0.05), the number of ACF per colon [157 +/- 7 and 198 +/- 14 (SE) in control and 3-OH-flavone groups, respectively, n = 10]. We also found that the number of "large" ACF was significantly increased in the group treated with 4-OH-benzoic acid. In conclusion, none of the phenolic compounds tested demonstrated a suppressive action on ACF induction by AOM.
Femia
A P
AP
Department of Pharmacology, University of Florence, 50139 Florence, Italy.
Caderni
G
G
Buzzigoli
C
C
Cocca
E
E
Salvadori
M
M
Dolara
P
P
D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage*; D001397:Azoxymethane; D003106:Colon / Q000473:pathology*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D003373:Coumaric Acids / Q000008:administration & dosage; D004032:Diet; D005419:Flavonoids / Q000008:administration & dosage; D062385:Hydroxybenzoates / Q000008:administration & dosage; D008297:Male; D010226:Parabens / Q000008:administration & dosage; D010636:Phenols / Q000008:administration & dosage*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D011916:Rats, Inbred F344
D016588:Anticarcinogenic Agents; D003373:Coumaric Acids; D005419:Flavonoids; D062385:Hydroxybenzoates; D010226:Parabens; D010636:Phenols; C032948:3-hydroxybenzoic acid; C038193:4-hydroxybenzoic acid; D001397:Azoxymethane; C429845:7-hydroxyflavone; C041477:3-hydroxyflavone