Dehalogenation of chlorinated ethenes and immobilization of nickel in anaerobic sediment columns under sulfidogenic conditions.
A sediment column study was carried out to demonstrate the bioremediation of chloroethene- and nickel-contaminated sediment in a single anaerobic step under sulfate-reducing conditions. Four columns (one untreated control column and three experimental columns) with sediment from a chloroethene- and nickel-contaminated site were investigated for 1 year applying different treatments. By stimulating the activity of sulfate-reducing bacteria by the addition of sulfate as supplementary electron acceptor, complex anaerobic communities were maintained with lactate as electron donor (with or without methanol), which achieved complete dehalogenation of tetra- and trichloroethenes (PCE and TCE) to ethene and ethane. A few weeks after sulfate addition, production of sulfide increased, indicating an increasing activity of sulfate-reducing bacteria. The nickel concentration in the effluent of one nickel-spiked column was greatly reduced, likely due to the enhanced sulfide production, causing precipitation of nickel sulfide. At the end of the study, 94% of the initial amount of nickel added to that column was recovered in the sediment As compared to the untreated (nonspiked) control column, all chloroethene-spiked columns ladditions of PCE and TCE) showed a permanent release of small chloride ion quantities (approximately 0.5-0.7 mM chloride), which were detected in the effluents a few weeks after sulfide production was observed for the first time. The formation of ethene and ethane as final products after dechlorination of PCE and TCE was detected in some effluents and in some gas phases of the columns. Other metabolites or intermediates (such as DCE isomers) were only detected sporadically in negligible quantities. The results of this study demonstrated thatmicrobial activity stimulated under sulfate-reducing conditions can have a beneficial effect on both the precipitation of heavy metals and the complete dechlorination of organochlorines. The strongly negative redox potential created by the activity of sulfate-reducing bacteria may be one factor responsible for stimulating the activity of the dehalogenating bacteria in the test columns.
Drzyzga
Oliver
O
Department of Marine Microbiology, University of Bremen, Germany. drzyzga@biotec.uni-bremen.de
El Mamouni
Rachid
R
Agathos
Spiros N
SN
Gottschal
Jan C
JC
D001421:Bacteria, Anaerobic / Q000502:physiology; D001673:Biodegradation, Environmental; D004785:Environmental Pollutants / Q000378:metabolism*; D019015:Geologic Sediments / Q000737:chemistry* / Q000382:microbiology; D007536:Isomerism; D009532:Nickel / Q000378:metabolism*; D010084:Oxidation-Reduction; D013440:Sulfides; D014752:Vinyl Chloride / Q000378:metabolism*
D004785:Environmental Pollutants; D013440:Sulfides; D009532:Nickel; D014752:Vinyl Chloride