title: The atopy patch test -- reproducibility and comparison of different evaluation methods.
abstract: BACKGROUND
  There is still a lack of standardization of the atopy patch test (APT) in test procedures and evaluation methods. Our aim was to examine the reproducibility of APT results and to compare visual evaluation to chromametry and laser Doppler imaging.
  
  
  METHODS
  Fifty-two volunteers with atopic eczema/dermatitis syndrome (AEDS) were included. The APT was performed on tape-stripped and unstripped test fields on their backs using cat dander, house dust mite and grass pollen allergens from two different suppliers. Responders were re-tested 4-12 weeks later with the same allergens on their forearms.
  
  
  RESULTS
  Using Allergopharma allergens, 14 (26.9%) volunteers showed one or more positive reactions. The reproducibility rate was 56.3%. The Erlangen atopy score in APT-positive and negative volunteers was 19 +/- 6 vs 15 +/- 6. The test agreement in volunteers tested with both allergens, from Allergopharma and Stallergènes, was poor. Correlation of the results between the three evaluation methods was significant (P < or = 0.001).
  
  
  CONCLUSIONS
  The low reproducibility rate of APT results and the poor inter-test-agreement using allergens from different suppliers show that much work remains to make the APT a reliable tool in identifying relevant aeroallergens that lead to flare ups of AEDS. Compared to chromametry and laser Doppler imaging, visual scoring was superior in differentiation between irritative and allergic reactions.
authors.affiliation: Department of Dermatology adn Allergology, University-Hospitals of the Friedrich Schiller University Jena, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Heinemann
authors.forename: S
authors.initials: S
authors.lastname: Schliemann-Willers
authors.forename: D
authors.initials: D
authors.lastname: Kelterer
authors.forename: U
authors.initials: U
authors.lastname: Metzner
authors.forename: K
authors.initials: K
authors.lastname: Kluge
authors.forename: W
authors.initials: W
authors.lastname: Wigger-Alberti
authors.forename: P
authors.initials: P
authors.lastname: Elsner
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000009:adverse effects* / Q000276:immunology; D000818:Animals; D002415:Cats; D003876:Dermatitis, Atopic / Q000175:diagnosis*; D004391:Dust / Q000009:adverse effects; D005260:Female; D006801:Humans; D008297:Male; D008925:Mites / Q000276:immunology; D010328:Patch Tests / Q000379:methods* / Q000592:standards*; D006109:Poaceae / Q000009:adverse effects / Q000276:immunology; D011058:Pollen / Q000009:adverse effects / Q000276:immunology; D015203:Reproducibility of Results; D013577:Syndrome
chemical_list: D000485:Allergens; D004391:Dust