Cellular localization of a chromogranin B-like derived peptides in leeches.
Using immunocytochemistry techniques, we demonstrated specific immunostaining with antibodies rose against fragment (547-560) and (614-626) of bovine chromogranin B (CGB) at the level of brain and the tegument of the leech Theromyzon tessulatum. The used of these antibodies on leech sections revealed immunopositive labeling in neurons and glial cells of the central nervous system (CNS), and epidermal glandular cells of the tegument. Co-localization between two antibodies rose against different fragment of bovine CGB have been demonstrated in neurons and glial cells of leech CNS like in vertebrates. Finally, the whole of the data showed for the first time the presence in leeches of CGB like derived peptides.
Salzet
Michel
M
Laboratoire de Neuroimmunologie des Annélides, UMR CNRS 8017, SN3, IFR 118, Université des Sciences et Technologies de Lille. 59650 Villeneuve d'Ascq, France. salzet@univ-lille1.fr
Verger-Bocquet
Martine
M
D000818:Animals; D000918:Antibody Specificity; D053378:Chromogranin B; D002864:Chromogranins / Q000032:analysis* / Q000276:immunology; D004817:Epidermis / Q000737:chemistry; D007150:Immunohistochemistry; D007865:Leeches / Q000737:chemistry*; D009420:Nervous System / Q000737:chemistry; D010446:Peptide Fragments / Q000032:analysis* / Q000276:immunology
D053378:Chromogranin B; D002864:Chromogranins; D010446:Peptide Fragments; C093477:secretolytin