title: Purification and characterization of heparinase that degrades both heparin and heparan sulfate from Bacillus circulans.
abstract: A heparinase that degrades both heparin and heparan sulfate (HS) was purified to homogeneity from the cell-free extract of Bacillus circulans HpT298. The purified enzyme had a single band on SDS-polyacrylamide gel electrophoresis with an estimated molecular mass of 111,000. The enzyme showed optimal activity at pH 7.5 and 45 degrees C, and its activity was stimulated in the presence of 5 mM CaCl2, BaCl2, or MgCl2. Analysis of substrate specificity and degraded disaccharides demonstrated that the enzyme acts on both heparin and HS, similar to heparinase II from Flavobacterium heparinum.
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Yoshida
authors.forename: Kazuya
authors.initials: K
authors.lastname: Sakai
authors.forename: Shinji
authors.initials: S
authors.lastname: Tokuyama
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Miyazono
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maruyama
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Morikawa
authors.forename: Keiichi
authors.initials: K
authors.lastname: Yoshida
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Tahara
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006493:Heparin / Q000378:metabolism*; D019763:Heparin Lyase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006497:Heparan Sulfate / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008970:Molecular Weight; D013379:Substrate Specificity
chemical_list: D006493:Heparin; D019763:Heparin Lyase; D006497:Heparan Sulfate