Two immunologically distinct types of protofilaments can be identified in Natrialba magadii flagella.
We examine distribution of flagellins along multicomponent flagellar filaments (FF) and protofilaments (PF) of the haloalkaliphilic archaeon Natrialba magadii using immunogold electron microscopy. A high specific polyclonal antibody raised to one of the flagellin types bound homogeneously to the undissociated FF along the full length. At the same time both uniformly labelled and completely unlabelled PF, outwardly indistinguishable one from another, were observed.
Pyatibratov
Michael G
MG
Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.
Leonard
Kevin
K
Tarasov
Valery Y
VY
Fedorov
Oleg V
OV
D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000276:immunology* / Q000648:ultrastructure*; D005408:Flagellin / Q000276:immunology / Q000378:metabolism*; D006216:Halobacteriaceae / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron
D005408:Flagellin