Plant-derived human papillomavirus 16 E7 oncoprotein induces immune response and specific tumor protection.
Vaccine strategies for treatment of human papillomavirus-induced cervical cancer are based on either the recombinant E7 fusion oncoprotein or E7 CTL peptides. The therapeutic potential of the E7-based vaccine depends on the use of different adjuvants. In this study, we describe for the first time the expression of the human papillomavirus 16 E7 protein in Nicotiana benthamiana plant using a potato virus X-derived vector. C57BL/6 mice immunized with E7-containing crude foliar extracts developed both humoral and cell-mediated immune responses and were protected from tumor development after challenge with the E7-expressing C3 tumoral cell line. Our data support the possibility of producing a cost-effective anticancer vaccine in plant with intrinsic adjuvant-like properties.
Franconi
Rosella
R
Ente per le Nuove Tecnologie, Energia e Ambiente, UTS Biotech, Sezione Genetica e Genomica Vegetale, CR Casaccia, Rome, Italy.
Di Bonito
Paola
P
Dibello
Francesco
F
Accardi
Luisa
L
Muller
Antonio
A
Cirilli
Alessia
A
Simeone
Paola
P
Donà
M Gabriella
MG
Venuti
Aldo
A
Giorgi
Colomba
C
D000818:Animals; D019496:Cancer Vaccines / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control / Q000821:virology*; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D050725:Papillomavirus E7 Proteins; D017863:Potexvirus / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014026:Nicotiana / Q000378:metabolism* / Q000821:virology
D019496:Cancer Vaccines; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16