Synthesis of Ser-His-Gly.DNA conjugates.
There are strong interactions between the bases of oligonucleotides. Based on Watson-Crick principle, they can form stable secondary and tertiary structure such as hairpin, duplex, triplex, G-quartet, pseudoknot, which can serve as the scaffold of molecules. Peptides contain active groups such as amino, carboxyl, imidazole, hydroxyl. A protected Ser-His-Gly-Threoninol phosphoramidite was synthesized in this work and was incorporated into a triplex-forming oligonucleotide. The results indicate that the oligonucleotide containing Ser-His-Gly-Threoninol in the middle could still form triplex with the target duplex and the dissociation constant was 0.5 micromol/L.
Li
Xi-Qing
XQ
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China.
Liu
Hai
H
Zhuang
Min
M
Tan
Bo
B
Zhao
Yu-Fen
YF
Lu
Chang-De
CD
D004247:DNA / Q000737:chemistry*; D005998:Glycine / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D012694:Serine / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet
D009841:Oligonucleotides; D009842:Oligopeptides; C051802:triplex DNA; D012694:Serine; D006639:Histidine; D004247:DNA; D005998:Glycine