The effect of nitrogen and carbon sources on growth of the biocontrol agent Pantoea agglomerans strain CPA-2.
OBJECTIVE
The effect of several nitrogen and carbon sources on the growth of Pantoea agglomerans (strain CPA-2) was studied for the first time.


RESULTS
Synthetic nitrogen and carbon sources were tested to obtain a suitable medium. Synthetic yeast extract provided maximum growth and disaccharides such as sucrose, lactose and trehalose improved this growth significantly from 3.2 x 10(9) to 5.5 x 10(9) cfu ml-1.


CONCLUSIONS
Pantoea agglomerans can be produced in a combination of nitrogen sources such as yeast extract with carbohydrates such as sucrose in shake flask and a laboratory fermenter (5 l).


CONCLUSIONS
Results suggest good production of this biocontrol agent on a laboratory scale and the potential of scaling up the process.
Costa
Elena
E
Postharvest Unit, CeRTA, Centre UdL-IRTA, Lleida, Spain. elena.costa@irta.es
Teixidó
N
N
Usall
J
J
Atarés
E
E
Viñas
I
I
D018533:Biomass; D002244:Carbon / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D020636:Pantoea / Q000187:drug effects / Q000254:growth & development*; D010572:Pest Control, Biological
D003470:Culture Media; D002244:Carbon; D009584:Nitrogen