title: Cloaking cytolytic peptides for liposome-based detection and potential drug delivery.
abstract: Potent cytolytic peptides with specific tethering and cloaking sites have been synthesised and used to release payload from liposomes in a quantitative manner. A functionally located cloaking site has been modified specifically by simple conjugation without adversely affecting the cytolytic properties of the peptide. The cytolytic activity of modified peptides was then efficiently (>98%) cloaked and uncloaked by ligand-protein or hapten-antibody interactions. The principle of a dual response peptide has been demonstrated using an avidin-cloaked pH-sensitive peptide. Biospecific cloaking/uncloaking provided a new sensitive (approximately 12 pmol) homogeneous diagnostic and also appears potentially suited to bioresponsively targeted release of antimicrobial, anticancer and other drugs now delivered using liposomes.
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, University of Manchester, UK.
authors.forename: Harmesh S
authors.initials: HS
authors.lastname: Aojula
authors.forename: Shaun
authors.initials: S
authors.lastname: Offerman
authors.forename: Raj R
authors.initials: RR
authors.lastname: Aojula
authors.forename: Alistair P
authors.initials: AP
authors.lastname: Hutchinson
authors.forename: Steve
authors.initials: S
authors.lastname: Nicklin
authors.forename: David J
authors.initials: DJ
authors.lastname: Clarke
mesh_terms: D000595:Amino Acid Sequence; D001360:Avidin; D001710:Biotin; D003603:Cytotoxins / Q000008:administration & dosage* / Q000138:chemical synthesis; D016503:Drug Delivery Systems; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D008555:Melitten / Q000008:administration & dosage / Q000138:chemical synthesis; D008969:Molecular Sequence Data; D010455:Peptides / Q000008:administration & dosage* / Q000138:chemical synthesis
chemical_list: D003603:Cytotoxins; D008081:Liposomes; D010455:Peptides; D001360:Avidin; D008555:Melitten; D001710:Biotin