[Influence of a bolus injection of thiopental and propofol on the expression density of P-selectin and fibrinogen receptors on the surface of human platelets ex vivo].
OBJECTIVE
Exposure of platelets to anaesthetic agents may alter their functional characteristics and in patients undergoing surgery or long-term sedation this may be clinically relevant. We investigated the influence of thiopentone and propofol on the expression of platelet membrane P-selectin, fibrinogen receptor, and leukocyte-platelet aggregates ex vivo.


METHODS
In patients undergoing surgery blood samples were taken from a peripheral vein cannula before induction of anaesthesia with propofol (3.0 mg kg (-1) BW) or thiopentone (7.0 mg kg (-1) BW) and five minutes later (n = 11 in each group). Surface expression of the fibrinogen receptor complex (GP Ib/IIIa), P-selectin, and the percentage of leukocyte-platelet aggregates were measured by flow cytometry after stimulation via the ADP or thrombin receptor pathway.


RESULTS
There was no significant difference in the expression of gpIIb/IIIa, P-selectin, and percentage of leukocyte-platelet aggregates in the thiopentone and propofol group after induction of anaesthesia.


CONCLUSIONS
In clinically relevant concentrations thiopentone and propofol did not influence the surface expression density of fibrinogen receptors, P-selectin molecules, and the percentage of leukocyte-platelet aggregates ex vivo.
Scheinichen
D
D
Zentrum Anästhesiologie, Medizinische Hochschule Hannover. scheinichen.dirk@mh-hannover.de
Bornscheuer
A
A
Jaeger
K
K
Mahr
K-H
KH
Koksch
M
M
Ruschulte
H
H
Heine
J
J
D018686:Anesthetics, Intravenous / Q000008:administration & dosage / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000096:biosynthesis*; D010974:Platelet Aggregation / Q000187:drug effects; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000096:biosynthesis* / Q000187:drug effects / Q000378:metabolism; D015742:Propofol / Q000008:administration & dosage / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000187:drug effects*; D013874:Thiopental / Q000008:administration & dosage / Q000494:pharmacology*
D018686:Anesthetics, Intravenous; D019007:P-Selectin; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D011956:Receptors, Cell Surface; D013874:Thiopental; D015742:Propofol