title: Determination of remifentanil in human blood by capillary gas chromatography with nitrogen-selective detection.
abstract: A validated method for the determination of remifentanil in human blood, applicable to all therapeutic concentrations, using capillary GC with nitrogen-specific detection and fentanyl as the internal standard has been developed. Citrated whole blood samples were extracted into 1-chlorobutane following precipitation of proteins with methanol. The drugs were back extracted into 10 mM HCl and re-extracted into methanol-1-chlorobutane. The extracts were reconstituted in methanol and injected onto a 25-m BPX-5 column. The lower limit of quantitation was 0.2 ng/ml with within- and between-day coefficients of variation of less than 15%.
authors.affiliation: Anaesthesia Research Laboratory, Department of Anaesthesia and Pain Management, The Royal Melbourne Hospital, Victoria 3050, Australia.
authors.forename: A R
authors.initials: AR
authors.lastname: Bjorksten
authors.forename: C
authors.initials: C
authors.lastname: Chan
authors.forename: D P
authors.initials: DP
authors.lastname: Crankshaw
mesh_terms: D000701:Analgesics, Opioid / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D009584:Nitrogen; D010880:Piperidines / Q000097:blood*; D000077208:Remifentanil; D015203:Reproducibility of Results
chemical_list: D000701:Analgesics, Opioid; D010880:Piperidines; D009584:Nitrogen; D000077208:Remifentanil