Immunophenotypic analysis of acute lymphocytic leukemia.
Acute lymphoblastic leukemia (ALL) is one of the most common hematologic malignancies. Flow cytometry is an integral part of ALL diagnosis and also provides significant patient prognostic information. This article is a practical review of the basic principles of the flow cytometric evaluation of acute leukemias, the interpretation of flow cytometric data, and the management of practical problems such as aberrant antigen, hematogones, bone marrow regeneration, and minimal residual disease.
Riley
Roger S
RS
Department of Pathology, Medical College of Virginia, Hospitals of Virginia Commonwealth University, 403 North 13th Street, Richmond, VA 23298-0250, USA. rsriley@hsc.vcu
Massey
Davis
D
Jackson-Cook
Colleen
C
Idowu
Michael
M
Romagnoli
Gina
G
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D000970:Antineoplastic Agents / Q000009:adverse effects / Q000627:therapeutic use; D001402:B-Lymphocytes / Q000737:chemistry / Q000276:immunology; D001853:Bone Marrow / Q000473:pathology / Q000528:radiation effects; D001856:Bone Marrow Examination / Q000379:methods; D016026:Bone Marrow Transplantation; D019070:Cell Lineage; D002648:Child; D003131:Combined Modality Therapy; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D005544:Forecasting; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D016130:Immunophenotyping; D018365:Neoplasm, Residual; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000276:immunology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000145:classification* / Q000175:diagnosis / Q000276:immunology / Q000473:pathology / Q000628:therapy; D011379:Prognosis; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology; D019172:Transplantation Conditioning / Q000009:adverse effects
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D006684:HLA-DR Antigens