title: The extraction of imperialine and imperialine-3 beta-glucoside from Fritillaria pallidiflora Schrenk and quantitative determination by HPLC-evaporative light scattering detection.
abstract: The extraction procedure and quantitative determination by HPLC-evaporative light scattering detection (ELSD) of the main bioactive components, namely, imperialine (1) and imperialine-3 beta-glucoside (2), of bulbs of Fritillaria pallidiflora Schrenk have been investigated. The most efficient method for the simultaneous extraction of 1 and 2 involved pre-treatment of the bulb powder with ammonia, followed by reflux with dichloromethane:methanol at 90 degrees C for 4 h. Simultaneous determination of non-chromophoric 1 and 2 by HPLC-ELSD employed a Kromasil C18 column eluted with acetonitrile:water:diethylamine. The assay was accurate and reproducible with an overall variation lower than 4% and a sample recovery higher than 98%. The methods described have been successfully used to evaluate the quality of three batches of the crude traditional Chinese medicinal herb derived from the bulbs of F. pallidiflora.
authors.affiliation: Department of Pharmacognosy, China Pharmaceutical University, Nanjing, 210009, People's Republic of China. lipingli@public1.ptt.js.cn
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Ling-Jie
authors.initials: LJ
authors.lastname: Zeng
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Li
authors.forename: Ge
authors.initials: G
authors.lastname: Lin
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D002595:Cevanes / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D031410:Fritillaria / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D008027:Light; D018514:Plant Structures / Q000737:chemistry; D012542:Scattering, Radiation
chemical_list: D000470:Alkaloids; D002595:Cevanes; D005960:Glucosides; C000601289:imperialine-3-glucoside; C068448:imperialine-beta-glucoside; C047331:peiminine