[High performance liquid chromatography and radioimmunoassay in analysis of serum hydrocortisone concentration and pharmacokinetic parameters of glucocorticoids in human. The choice of method for clinical practice].
Serum hydrocortisone concentrations were measured by high performance liquid chromatography (HPLC) and radioimmunoassay (RIA) in 24 children with different diseases and 16 healthy adults. HPLC and RIA proved to be equally sensitive. However the authors recommend HPLC as a reliable, rapid, and ecologically pure method, which is, in addition, selective and economic.
Chekalin
A F
AF
Rossokhin
V F
VF
Nesterov
S L
SL
Kazanskiĭ
A S
AS
Salmin
A A
AA
Shishkanov
S F
SF
Kazakova
M A
MA
Kozlova
E M
EM
Eliseeva
M V
MV
Sedyshev
V I
VI
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005938:Glucocorticoids / Q000097:blood* / Q000493:pharmacokinetics*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D008875:Middle Aged; D011863:Radioimmunoassay / Q000379:methods; D012680:Sensitivity and Specificity
D005938:Glucocorticoids; D006854:Hydrocortisone