[Effects of chronic cadmium loading on the testis and endocrine function of reproduction in male rats].
Sixty healthy Sprague-Dawley male rats were used and divided randomly into control group (group C), cadmium loading group with medium dose (group M) and cadmium loading group with high dose (group H). Groups C, M and H were orally dosed daily with 0, 5 and 10 mg/kg of cadmium for over 6 weeks. Effects of cadmium loading on testis and endocrine function of reproduction in male rats were studied. The results showed that the zinc content decreased slightly in testis and plasma, and the cadmium concentration increased significantly in the testis of groups M and H; while the plasma levels of cadmium and zinc had no obvious difference as compared with those of group C; daily sperm production in the testis of group H decreased markedly during week 3 of cadmium loading, and was significantly lower in groups M and H as compared to that in group C during week 6; alkaline phosphatase (ALP) in group H and lactate dehydrogenase-X (LDH-X) in groups M and H were markedly lower compared to those of group C; plasma testosterone (T) level in both cadmium loading groups decreased and was low or significantly lower than that in group C; follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels had no apparent difference between the three groups. It is suggested that the gradual accumulation of cadmium in testis tissue induced by chronic cadmium loading results in changes in some enzyme activity, a decrease in sperm production, and defect of endocrine function activity in the testis.
Chen
Long
L
College of Life Science, Nanjing Normal University, Nanjing 210097, China. Lchen@njnu.edu.cn
Ren
Wen-Hua
WH
Zhu
Shan-Liang
SL
Gao
Wei
W
Zhou
Juan
J
Jiang
Ying-Zi
YZ
Gu
Yu
Y
D000469:Alkaline Phosphatase / Q000187:drug effects; D000818:Animals; D002104:Cadmium / Q000097:blood; D019256:Cadmium Chloride / Q000008:administration & dosage / Q000633:toxicity*; D005640:Follicle Stimulating Hormone / Q000097:blood; D007527:Isoenzymes / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000187:drug effects; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000201:enzymology / Q000473:pathology*; D013739:Testosterone / Q000097:blood*
D007527:Isoenzymes; D002104:Cadmium; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D007770:L-Lactate Dehydrogenase; C409673:lactate dehydrogenase C4; D000469:Alkaline Phosphatase; D019256:Cadmium Chloride