title: Folding and oxidation of the antibody domain C(H)3.
abstract: The non-covalent homodimer formed by the C-terminal domains of the IgG1 heavy chains (C(H)3) is the simplest naturally occurring model system for studying immunoglobulin folding and assembly. In the native state, the intrachain disulfide bridge, which connects a three-stranded and a four-stranded beta-sheet is buried in the hydrophobic core of the protein. Here, we show that the disulfide bridge is not required for folding and association, since the reduced C(H)3 domain folds to a dimer with defined secondary and tertiary structure. However, the thermodynamic stability of the reduced C(H)3 dimer is much lower than that of the oxidized state. This allows the formation of disulfide bonds either concomitant with folding (starting from the reduced, denatured state) or after folding (starting from the reduced dimer). The analysis of the two processes revealed that, under all conditions investigated, one of the cysteine residues, Cys 86, reacts preferentially with oxidized glutathione to a mixed disulfide that subsequently interacts with the less-reactive second thiol group of the intra-molecular disulfide bond. For folded C(H)3, the second step in the oxidation process is slow. In contrast, starting from the unfolded and reduced protein, the oxidation reaction is faster. However, the overall folding reaction of C(H)3 during oxidative folding is a slow process. Especially, dimerization is slow, compared to the association starting from the denatured oxidized state. This deceleration may be due to misfolded conformations trapped by the disulfide bridge.
authors.affiliation: Institut für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching, Germany.
authors.forename: Michael J W
authors.initials: MJ
authors.lastname: Thies
authors.forename: Fabio
authors.initials: F
authors.lastname: Talamo
authors.forename: Marcus
authors.initials: M
authors.lastname: Mayer
authors.forename: Stefan
authors.initials: S
authors.lastname: Bell
authors.forename: Margherita
authors.initials: M
authors.lastname: Ruoppolo
authors.forename: Gennaro
authors.initials: G
authors.lastname: Marino
authors.forename: Johannes
authors.initials: J
authors.lastname: Buchner
mesh_terms: D000478:Alkylation; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000378:metabolism*; D002850:Chromatography, Gel; D002942:Circular Dichroism; D003432:Cross-Linking Reagents; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism*; D007127:Immunoglobulin Constant Regions / Q000737:chemistry / Q000378:metabolism*; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry; D008958:Models, Molecular; D010084:Oxidation-Reduction; D010449:Peptide Mapping; D011489:Protein Denaturation; D017510:Protein Folding; D020673:Protein Renaturation; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D014461:Ultracentrifugation
chemical_list: D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D004220:Disulfides; D007127:Immunoglobulin Constant Regions; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D003545:Cysteine