Application of a normalised plot to the study of uni-uni enzyme-inhibitor systems.
Normalisation of kinetic data is a useful tool in the study of complex enzyme systems. In the present paper, we have applied the premises of the normalised plot to the description of uni-uni enzyme inhibition. Guidelines to the design of the experiments and to data managing using the freeware program SIMFIT (http:\\www.simfit.man.ac.uk) are offered. The treatment has a lessened demand in experimental data while ensuring biological consistence of the results. Moreover, the results are obtained without resorting to secondary plots, and the election between rival mechanisms is statistically granted. Hyperbolic mixed-type inhibition is studied as a general model for enzyme-inhibitor/activator interaction, and equations describing classical cases of linear inhibition are also considered.
Bravo
Ignacio G
IG
Dipartmento Bioquímica y Biología Molecular, Universidad de León, Campus Vegazana, 24071, León, Spain.
Busto
Félix
F
De Arriaga
Dolores
D
Ferrero
Miguel A
MA
Rodríguez-Aparicio
Leandro B
LB
Martínez-Blanco
Honorina
H
Reglero
Angel
A
D004791:Enzyme Inhibitors / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry*; D007700:Kinetics; D008433:Mathematics; D008956:Models, Chemical; D015203:Reproducibility of Results
D004791:Enzyme Inhibitors; D004798:Enzymes