title: Amiodarone induces apoptosis in L-132 human lung epithelial cell line.
abstract: To investigate whether amiodarone induces apoptosis in cells of the L-132 human lung epithelial cell line, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, 4,6-diamidino-2-phenylindole staining, DNA fragmentation assay, reverse transcription-polymerase chain reaction, and casapse-3 enzyme assay were performed. Through morphological and biochemical analyses, it was demonstrated that L-132 cells treated with amiodarone exhibit several features of apoptosis. In addition, it was shown that amiodarone increases the mRNA levels of bax and caspase-3. Based on the results, amiodarone appears to activate specific intracellular death-related pathways, including possibly the bax-dependent caspase-3 activation pathway, and thus induce apoptosis in human lung epithelial cells.
authors.affiliation: Department of General Thorax and Cardiovascular Surgery, College of Medicine, Kyung Hee University, Seoul, South Korea.
authors.forename: In-Serk
authors.initials: IS
authors.lastname: Choi
authors.forename: Bum-Shik
authors.initials: BS
authors.lastname: Kim
authors.forename: Kyu-Seok
authors.initials: KS
authors.lastname: Cho
authors.forename: Joo-Chul
authors.initials: JC
authors.lastname: Park
authors.forename: Mi-Hyeon
authors.initials: MH
authors.lastname: Jang
authors.forename: Min-Chul
authors.initials: MC
authors.lastname: Shin
authors.forename: Sae-Bin
authors.initials: SB
authors.lastname: Jung
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Chung
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000638:Amiodarone / Q000633:toxicity*; D000889:Anti-Arrhythmia Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D004396:Coloring Agents; D053938:DNA Fragmentation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D007202:Indicators and Reagents; D008168:Lung / Q000166:cytology* / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000889:Anti-Arrhythmia Agents; D004396:Coloring Agents; D007202:Indicators and Reagents; D012333:RNA, Messenger; D013778:Tetrazolium Salts; D013844:Thiazoles; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; C022616:thiazolyl blue; D000638:Amiodarone