Association of a new c-Cbl related protein with the very first stages of apoptosis induction.
This study investigates the involvement of the c-cbl proto-oncogene during the first stages of the apoptotic process. We have already shown that a c-Cbl aptotosis-related protein of 90 kDa (CARP 90) is detected very rapidly in the cytoplasm as well as in the nucleus of murine thymocytes after hydrocortisone (HC) treatment. We report here that this protein appeared as well after in vivo treatment of mice by gamma-irradiation or injection of anti-CD3 monoclonal antibody, two potent thymic apoptosis inductors, providing a close relationship between the occurrence of apoptosis and the appearance of CARP 90. We showed that CARP 90 and p120(cbl) share numerous epitopes strikingly suggesting that CARP 90 is coded by c-cbl. In addition, KO mice do not sustain CARP 90 appearance. We finally showed that CARP 90 contains N- and C-terminal end epitopes of p120(cbl), which suggests that CARP 90 is an alternative spliced form of c-cbl. This protein was also observed under gamma-irradiation in tissues of different origin, which enlarges the physiological significance of this phenomenon. The very rapid CARP 90 appearance under apoptotic conditions in the nucleus of cells originating in different tissues makes this protein if not a possible new actor of the apoptotic process, at least an interesting marker of this process.
Corsois
Laurent
L
Laboratory of Molecular Oncology, CNRS-UMR 8526, Institut Pasteur de Lille, France.
Quatannens
Brigitte
B
Dumont
Patrick
P
Aumercier
Marc
M
Defresne
Marie-Paule
MP
Régnier
Daniel C L
DC
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D015153:Blotting, Western; D017252:CD3 Complex / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005720:Gamma Rays; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D019169:Jurkat Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D018345:Mice, Knockout; D011233:Precipitin Tests; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D050721:Proto-Oncogene Proteins c-cbl; D013950:Thymus Gland / Q000378:metabolism / Q000473:pathology*; D044767:Ubiquitin-Protein Ligases
D000893:Anti-Inflammatory Agents; D017252:CD3 Complex; D000939:Epitopes; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D050721:Proto-Oncogene Proteins c-cbl; D044767:Ubiquitin-Protein Ligases; C067419:CBL protein, human; C493849:Cbl protein, mouse; D006854:Hydrocortisone