title: Crystallographic study of a naturally occurring trans-splicing intein from Synechocystis sp. PCC 6803.
abstract: A naturally occurring split intein from the dnaE gene of Synechocystis sp. PCC6803 (Ssp DnaE intein) has been purified and crystallized using PEG 8K as precipitant. The crystal belongs to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 58.5, c = 70.2 A. It has one molecule per asymmetric unit and diffracts to beyond 2.9 A under cryoconditions (110 K) using a Cu rotating-anode X-ray generator in-house.
authors.affiliation: Laboratory of Structural Biology, Department of Biological Science and Technology and MOE Laboratory of Protein Science, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Chen
authors.forename: Ming Qun
authors.initials: MQ
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.forename: Sébastien
authors.initials: S
authors.lastname: Ferrandon
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
mesh_terms: D017398:Alternative Splicing; D018360:Crystallography, X-Ray / Q000379:methods*; D000458:Cyanobacteria / Q000737:chemistry* / Q000378:metabolism; D004258:DNA Polymerase III / Q000737:chemistry* / Q000302:isolation & purification; D010957:Plasmids / Q000378:metabolism; D013696:Temperature
chemical_list: C074904:DNA polymerase III, alpha subunit; D004258:DNA Polymerase III