Analysis of gene expression following spinal cord injury in rat using complementary DNA microarray.
To identify genes that were altered by spinal cord injury (SCI), we used complementary DNA microarray consisting 1176 rat genes. Rats were subjected to contusive injury of the thoracic spinal cord. Sham animals received only a laminectomy. Twenty-four hours later, spinal cord was dissected out, a 32P labeled probe was prepared and hybridized to the microarray. We identified three genes that showed a greater than 2-fold increase in SCI tissue, heat shock 27-kDa protein, tissue inhibitor of metalloproteinase-1 and epidermal fatty acid-binding protein. Seven genes, lecithin:cholesterol acyltransferase, dipeptidyl aminopeptidase related protein, phospholipase C delta 4, plasma membrane Ca2+-ATPase isoform 2, G-protein GO alpha subunit, GABA transporter 3, and neuroendrocrine protein 7B2 were down-regulated greater than 50% in SCI tissue. Changes in expression of these genes were confirmed by reverse transcription-polymerase chain reaction. These genes may play a role in the response to tissue damage or repair following SCI.
Tachibana
Toshiya
T
Cellular Neuroscience Section, Pain and Neurosensory Mechanisms Branch NIDCR, NIH, Bethesda, MD 20892, USA. tachi@hyo-med.ac.jp
Noguchi
Koichi
K
Ruda
M A
MA
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D027682:Cation Transport Proteins; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins; D020411:Oligonucleotide Array Sequence Analysis; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000235:genetics; D053499:Plasma Membrane Calcium-Transporting ATPases; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000502:physiology; D013119:Spinal Cord Injuries / Q000503:physiopathology*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics
C506961:Atp2b2 protein, rat; D002352:Carrier Proteins; D027682:Cation Transport Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486114:Hspb1 protein, rat; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D019715:Tissue Inhibitor of Metalloproteinase-1; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D053499:Plasma Membrane Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases