[Expression of type I collagen and its receptor system in osteoblasts].
OBJECTIVE
To study the expression of type I collagen and its receptor system-integrin alpha 2 beta 1 in different passages of osteoblasts.


METHODS
The expression of type I collagen and integrin alpha 2 beta 1 in the primary, sixth and fifteenth passage of osteoblasts were detected by S-P immunohistological staining technique, and their mRNA expression by quantity RT-PCR technique.


RESULTS
Type I collagen and integrin alpha 2 beta 1 were expressed in different passages of osteoblasts and there was no significant difference among three passages by immunohistological technique. Their mRNA expression was gradually decreased with subculture.


CONCLUSIONS
Type I collagen promotes the adhesion and phenotype expression of osteoblasts through its receptor-integrin alpha 2 beta 1. The reductive expression of type I collagen-receptor system will decline the phenotype of osteoblasts.
Yu
X J
XJ
Department of Orthopedic Surgery, First University Hospital, West China University of Medical Sciences, Chengdu, Sichuan, P. R. China 610041.
Yang
Z M
ZM
Qu
Y
Y
D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D038982:Integrin alpha2beta1 / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D023822:Tissue Engineering
D024042:Collagen Type I; D038982:Integrin alpha2beta1; D012333:RNA, Messenger