[The effect of cefazolin loaded bone matrix gelatin on repairing large segmental bone defects and preventing infection after operation].
OBJECTIVE
To explore the possibility of repair long segmental bone defects and preventing infection with cefazolin loaded bone matrix gelatin (C-BMG).


METHODS
C-BMG was made from putting cefazolin into BMG by vacuum adsorption and freeze-drying techniques. The sustaining period of effective drug concentration in vitro and in vivo was detected by inhabition bacteria, and the drug concentration in local tissues (bone and muscle) and plasma after implantation of C-BMG was examined by high performance liquid chromatography(HPLC).


RESULTS
The effective inhibition time to staphylococcus aureus of C-BMG was 22 days in vitro, while 14 days in vivo. The drug concentration in local tissues(bone and muscle) were higher than that of plasma, and the drug concentration in local tissues was higher in early stage, later it kept stable low drug release. It suggested that C-BMG had excellent ability to repair segmental long bone defects.


CONCLUSIONS
C-BMG can gradually release cefazolin with effective drug concentration and has excellent ability to repair segmental long bone defects. It may be a novel method to repair segmental long bone defects and prevent infection after the operation.
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Department of Orthopedic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, P. R. China 524001.
Chen
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AM
D000818:Animals; D001857:Bone Matrix; D018786:Bone Substitutes / Q000627:therapeutic use; D002437:Cefazolin / Q000494:pharmacology* / Q000627:therapeutic use; D002511:Cephalosporins / Q000494:pharmacology* / Q000627:therapeutic use; D005780:Gelatin; D008826:Microbial Sensitivity Tests; D010012:Osteogenesis; D019736:Prostheses and Implants; D011817:Rabbits; D011885:Radius Fractures / Q000601:surgery*; D013211:Staphylococcus aureus / Q000187:drug effects; D013530:Surgical Wound Infection / Q000517:prevention & control*
D018786:Bone Substitutes; D002511:Cephalosporins; D005780:Gelatin; D002437:Cefazolin