Does chloroquine really act through oxidative stress?
To assess whether molecular oxygen and oxidative stress contribute to chloroquine activity, we cultivated strains of Plasmodium falciparum in erythrocytes with carboxyhemoglobin and an atmosphere containing 2% CO, 5% CO(2) and 93% N(2). Results indicate that, contrary to common belief, oxygen is not involved in the activity of chloroquine. Reactive radicals formation is suggested.
Monti
Diego
D
Department of Organic Chemistry, University of Milan, CNR-ISTM, Via Venezian 21, Italy. diego.monti@unimi.it
Basilico
Nicoletta
N
Parapini
Silvia
S
Pasini
Erica
E
Olliaro
Piero
P
Taramelli
Donatella
D
D000818:Animals; D000962:Antimalarials / Q000737:chemistry / Q000494:pharmacology*; D002245:Carbon Dioxide; D002248:Carbon Monoxide; D002263:Carboxyhemoglobin; D002738:Chloroquine / Q000737:chemistry / Q000494:pharmacology*; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology; D006801:Humans; D015394:Molecular Structure; D009584:Nitrogen; D018384:Oxidative Stress; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000378:metabolism
D000962:Antimalarials; D002245:Carbon Dioxide; D002248:Carbon Monoxide; D002738:Chloroquine; D002263:Carboxyhemoglobin; D009584:Nitrogen