[Sequencing and homology analysis of cDNAs encoding FhGST and AsGST].
OBJECTIVE
To study the homologies between FhGST, AsGST and Sj26GST.


METHODS
Total RNAs were extracted from Fasciola hepatica (Fh) and Ascaris suum (As) by guanidine hydrochloride/CsCl ultra-centrifugation. Two pairs of primers were designed according to FhGST and AsGST sequences. RT-PCR was carried out using total RNA as template. PCR products were directly sequenced. Then the homology analysis was made using DNASIS software on nucleotide level.


RESULTS
A FhGST403 bp fragment and a AsGST411 bp fragment were obtained and they both located in the open reading frame.


CONCLUSIONS
On nucleotide level, the homologies are 59% and 54% between the two cloned fragments and Schistosoma japonicum 26 kDa GST (Sj26GST) respectively.
Sun
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W
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Liu
S
S
D000818:Animals; D017165:Ascaris suum / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005210:Fasciola hepatica / Q000201:enzymology* / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D005982:Glutathione Transferase