[Measurement of cyclosporine A in whole blood by high performance liquid chromatography(HPLC)].
Whole blood was extracted by ether under base using cyclosporine D as internal standard. The extraction was evaporated and reconstituted. The interferences were further removed by hexane. The reconstitution was injected into a C18 reversed-phase column at 70 degrees C, which was eluted with a mobile phase of acetonitrite/methanol/water(60/10/30, v/v) at flow rate 1.6 ml.min-1 and monitored at 210 nm. Three levels of BIO-RAD cyclosporine A(whole blood) calibrator were measured, all the results were within expected range. The detection limit was 25 ng.ml-1. The linearity was at least within 50-1,000 ng.ml-1. Analytical recoveries were 99.6%-108.8%. The within-run cv and between-run CV were 7.2% and 9.3%, respectively.
Zeng
L
L
Department of Clinical Laboratory, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Huang
Y
Y
Peng
G
G
D002851:Chromatography, High Pressure Liquid; D016572:Cyclosporine / Q000097:blood*; D006801:Humans
D016572:Cyclosporine