Reticulocyte analysis by flow cytometry and other techniques.
Enumeration of peripheral blood reticulocytes is an essential part of the diagnosis and management of anemic patients, since the number of reticulocytes in the peripheral blood reflects the erythrocytic activity of the bone marrow. Reticulocyte enumeration using flow cytometric methodology is rapidly replacing the inaccurate, imprecise manual counting technique used in the past. This article explores the pathophysiology of the reticulocyte, the various means of counting reticulocytes, and the diverse clinical applications of reticulocyte data.
Riley
Roger S
RS
Department of Pathology, Medical College of Virginia, Hospitals of Virginia Commonwealth University, 403 North 13th Street, Richmond, VA 23298, USA. rsriley@hsc.vcu
Ben-Ezra
Jonathan M
JM
Tidwell
Ann
A
Romagnoli
Gina
G
D000911:Antibodies, Monoclonal / Q000276:immunology; D015415:Biomarkers; D001798:Blood Proteins / Q000032:analysis / Q000276:immunology; D001853:Bone Marrow / Q000473:pathology; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D005544:Forecasting; D006402:Hematologic Diseases / Q000097:blood / Q000175:diagnosis; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood; D008751:Methylene Blue; D009391:Nephelometry and Turbidimetry / Q000379:methods; D012313:RNA / Q000097:blood; D017701:Reticulocyte Count / Q000295:instrumentation / Q000379:methods*; D012156:Reticulocytes / Q000737:chemistry* / Q000502:physiology; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D001798:Blood Proteins; D005456:Fluorescent Dyes; D012313:RNA; D008751:Methylene Blue