[Experimental studies on hyperthermic distilled water combining with saline and dextran for preventing intraperitoneal cancer recurrence due to neoplasm seeding].
In order to know the efficacy of hyperthermic distilled water combining with saline and dextran (DSD) to prevent intraperitoneal cancer from recurrence due to neoplasm seeding, we carried out the experimental studies, including an observation on inhibiting cancer cells from implantation in vivo by abdominal cavity lavation, cytotoxic test and attachment test in vitro. The results of the tests in vitro showed no viable tumor cell exposed to 45 degrees C distilled water for 20 minutes in the tissue culture wells, meanwhile, dextran-40 partially blocked attachment of B16-F10 cells to gelatinized wells. In vivo test, DSD effectively prevented neoplasm seeding of B16-F10 viable melanoma cells in C57BL/6j mouse abdominal cavity and had no obvious toxicity. The efficiency was 70%; when cisplatin was added, the efficiency was enhanced to 88.9% with slight toxicity. These data indicate that the local application of hyperthermic distilled water combined with saline and DSD may prevent intraperitoneal neoplasm from recurring.
Pi
Z
Z
Department of Surgery, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
Wang
X
X
Liu
G
G
Zhou
J
J
Li
T
T
D000818:Animals; D003911:Dextrans / Q000494:pharmacology*; D005260:Female; D006979:Hyperthermia, Induced; D008297:Male; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000517:prevention & control; D009366:Neoplasm Seeding; D010534:Peritoneal Neoplasms / Q000517:prevention & control*; D012965:Sodium Chloride / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014867:Water
D003911:Dextrans; D014867:Water; D012965:Sodium Chloride