Effects of MCH/NEI in the striatum and interactions between them and with alpha-MSH on IP3 levels.
In this study, we investigated: (a) the effect of melanocyte concentrating hormone (MCH) and neuropeptide glutamine (E)-isoleusine-(I) (NEI) on IP(3) production on an "in vitro" model using slices containing caudate putamen (CP) and accumbens nuclei (ACC); and (b) the interaction between these peptides and with alpha-melanocyte-stimulating-hormone (alpha-MSH) on the production of this second messenger. Only MCH at the highest dose studied (3.6 microM) increased the production of IP(3), whereas at the low concentration (0.6 microM) it did not affect IP(3) levels. NEI and alpha-MSH at both concentrations tested (0.6 and 3.6 microM), did not affect IP(3) production either. However, when NEI or alpha-MSH (at 3.6 microM) were added together with 3.6 microM MCH, the increase in the IP(3) content induced by this last peptide was blocked.
Sanchez
Mónica Silvina
MS
Laboratorio de Fisiología, Departmento de Farmacología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Casilla de Correos 61, Agencia Postal 4, Córdoba, Argentina.
Berberian
Victoria
V
Celis
María Ester
ME
D000818:Animals; D004347:Drug Interactions; D007028:Hypothalamic Hormones / Q000494:pharmacology*; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D008297:Male; D008543:Melanins / Q000494:pharmacology*; D017072:Neostriatum / Q000187:drug effects* / Q000378:metabolism*; D009479:Neuropeptides / Q000494:pharmacology*; D010907:Pituitary Hormones / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000521:alpha-MSH / Q000494:pharmacology*
D007028:Hypothalamic Hormones; D008543:Melanins; D009479:Neuropeptides; D010907:Pituitary Hormones; D000521:alpha-MSH; C019463:melanin-concentrating hormone; D015544:Inositol 1,4,5-Trisphosphate