The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS-mediated embryo patterning.
Developmental responses to the plant hormone auxin are thought to be mediated by interacting pairs from two protein families: short-lived inhibitory IAA proteins and ARF transcription factors binding to auxin-response elements. monopteros mutants lacking activating ARF5 and the auxin-insensitive mutant bodenlos fail to initiate the root meristem during early embryogenesis. Here we show that the bodenlos phenotype results from an amino-acid exchange in the conserved degradation domain of IAA12. BODENLOS and MONOPTEROS interact in the yeast two-hybrid assay and the two genes are coexpressed in early embryogenesis, suggesting that BODENLOS inhibits MONOPTEROS action in root meristem initiation.
Hamann
Thorsten
T
ZMBP, Entwicklungsgenetik, Universität Tübingen, D-72076 Tübingen, Federal Republic of Germany.
Benkova
Eva
E
Bäurle
Isabel
I
Kientz
Marika
M
Jürgens
Gerd
G
D017360:Arabidopsis / Q000196:embryology / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation; D017343:Genes, Plant; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000254:growth & development*; D014157:Transcription Factors / Q000502:physiology*
D029681:Arabidopsis Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; C111371:MONOPTEROS protein, Arabidopsis; D014157:Transcription Factors