Role of PTPase(s) in regulating Fyn kinase at fertilization of the zebrafish egg.
Fertilization involves the activation of Src-family protein kinases which play a role at multiple stages of the egg activation process. The objective of the present study was to determine the mechanism by which one of these kinases, the Fyn kinase, is activated in response to fertilization of the zebrafish egg. Inhibitor studies demonstrated that many aspects of egg activation, including Fyn activation, require phosphotyrosyl phosphatase activity. A phosphotyrosyl phosphatase was found to be tightly associated with Fyn kinase and this interaction was mapped to the SH2 domain of Fyn. Coimmunoprecipitation studies identified rPTPalpha as a phosphatase that is complexed with Fyn in the egg, raising the possibility that rPTPalpha is part of the regulatory mechanism responsible for activating Fyn at fertilization.
Wu
Wenjun
W
Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas, 66160-7400, USA.
Kinsey
William H
WH
D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D002462:Cell Membrane / Q000201:enzymology; D004789:Enzyme Activation; D005306:Fertilization; D010063:Ovum / Q000201:enzymology / Q000254:growth & development; D011233:Precipitin Tests; D011485:Protein Binding; D017027:Protein Tyrosine Phosphatases / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000378:metabolism*; D051076:Proto-Oncogene Proteins c-fyn; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4; D011956:Receptors, Cell Surface; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism; D029961:Zebrafish Proteins
D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; D029961:Zebrafish Proteins; D051076:Proto-Oncogene Proteins c-fyn; C494964:fyna protein, zebrafish; D017027:Protein Tyrosine Phosphatases; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4; C516847:ptpra protein, zebrafish