Effects of exposure of Mya arenaria and Mactromeris polynyma to contaminated marine sediments on phagocytic activity of hemocytes.
Two species of bivalves, Mya arenaria and Mactromeris polynima, were exposed to contaminated marine sediments from Baie des Anglais, Quebec, for a period of 10 and 12 weeks, respectively, in order to determine if there was an effect on the phagocytic activity of hemocytes from each species. These sediments contain elevated levels of both PAHs and PCBs. Uncontaminated beach sand was used as control sediments. After a period of 4 weeks, each species of bivalves were sampled and hemocyte phagocytic activity was monitored by flow cytometry. While phagocytosis by hemocytes from M. polytiyma was significantly suppressed, those from M. arenaria were not different from beach sand-exposed controls. At the end of the exposure period, the phagocytic activity of hemocytes from both species was suppressed. Physiological parameters such as mantle proteins or malondialdehyde levels, total protein and total glycogen levels in the digestive gland were not affected by exposure to contaminated sediments. Moreover, the suppression of phagocytosis was well correlated with the transfer of contaminants from the sediments to the bivalves and their subsequent bioaccumulation, as demonstrated by the PCB body burden. These results support the use of bivalves as good sentinel species to survey sediment contamination and the usefulness of hemocyte phagocytic activity as a sensitive biomarker of exposure to organic contaminants.
Fournier
M
M
INRS-Institut Armand-Frappier Santé humaine, 245 Hymus Boul., Que., Pointe-Claire, Canada.
Pellerin
J
J
Lebeuf
M
M
Brousseau
P
P
Morin
Y
Y
Cyr
D
D
D000818:Animals; D049872:Bivalvia / Q000737:chemistry / Q000187:drug effects*; D004781:Environmental Exposure; D005434:Flow Cytometry; D006434:Hemocytes / Q000187:drug effects*; D010586:Phagocytes / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects*; D011078:Polychlorinated Biphenyls / Q000009:adverse effects / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects / Q000032:analysis; D011792:Quebec; D012989:Soil Pollutants / Q000009:adverse effects / Q000032:analysis; D014877:Water Pollution, Chemical / Q000009:adverse effects / Q000032:analysis
D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D011078:Polychlorinated Biphenyls