title: CancerB increases production of nitric oxide and tumor necrosis factor-alpha in peritoneal macrophages.
abstract: BACKGROUND
  CancerB (CCB, IMSF-5), herbal combination, may be able to stimulate potential toxic mediators such as nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) in isolated mouse peritoneal macrophages.
  
  
  METHODS
  NO production was determined by Griess method, and TNF-alpha production by enzyme-linked immunosorbent assay. Amounts of proteins were observed by Western blotting.
  
  
  RESULTS
  CCB had no effect on NO production by itself, but CCB alone did stimulate the production of TNF-alpha. When CCB was used in combination with recombinant interferon-gamma (rIFN-gamma), there was a marked cooperative induction of NO production, TNF-alpha production and NF-kappa B activation. The increase in NO synthesis was reflected as an increased amount of inducible NO synthase protein. The increased production of NO from rIFN-gamma plus CCB-stimulated peritoneal macrophages was decreased by the treatment with N(G)-monomethyl-L-arginine or N(alpha)-Tosyl-Phe Chloromethyl Ketone. Nuclear factor-kappa B (NF-kappa B) inhibitor, pyrrolidine dithiocarbamate was able to completely inhibit the production of NO and TNF-alpha.
  
  
  CONCLUSIONS
  These findings demonstrate that CCB increases the production of NO and TNF-alpha by rIFN-gamma-primed peritoneal macrophages and suggest that NF-kappa B plays a critical role in mediating these effects of CCB.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Korea Institute of Oriental Pharmacy, Wonkwang University, Iksan, Chonbuk, 570-749, Republic of Korea.
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Hwan-Suck
authors.initials: HS
authors.lastname: Chung
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Ho-Jeong
authors.initials: HJ
authors.lastname: Na
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Koo
authors.forename: Sang-Bong
authors.initials: SB
authors.lastname: Seo
authors.forename: Kyung-Suk
authors.initials: KS
authors.lastname: Kim
authors.forename: Yung-Sun
authors.initials: YS
authors.lastname: Song
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D029001:Herbal Medicine; D007371:Interferon-gamma / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism*; D008518:Medicine, East Asian Traditional; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D011759:Pyrrolidines / Q000494:pharmacology; D011994:Recombinant Proteins; D013859:Thiocarbamates / Q000494:pharmacology; D014108:Tosylphenylalanyl Chloromethyl Ketone / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D019323:omega-N-Methylarginine / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D011759:Pyrrolidines; D011994:Recombinant Proteins; D013859:Thiocarbamates; D014409:Tumor Necrosis Factor-alpha; C020972:pyrrolidine dithiocarbamic acid; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D014108:Tosylphenylalanyl Chloromethyl Ketone; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase