Nickel-induced plasma lipid peroxidation and effect of antioxidants in human blood: involvement hydroxyl radical formation and depletion of alpha-tocopherol.
To provide evidence for the oxidative effect of nickel (Ni) treatment on blood, lipid peroxidation (LPO) and hydroxyl radical (*OH) generation were examined in human plasma. Nickel chloride induced LPO in plasma of human blood in vitro in a concentration-dependent (0-10 mM) and time-dependent (0-2 h) manner. The *OH production in plasma was quantified by measurement of conversion of salicylic acid (SA) into its hydroxylated products, 2,3- and 2,5-dihydroxybenzoate (DHB). The concentrations of 2,3- and 2,5-DHB in plasma increased in a concentration-dependent manner after Ni treatment for 1 h. Furthermore, a decreasing trend in alpha-tocopherol levels in plasma was observed after Ni treatment. Concurrent incubation with gluthathione (GSH), catechin (CTCH), and mannitol decreased lipid peroxidation and reduced *OH formation induced by Ni, but exacerbation of the decrease of alpha-tocopherol in plasma occurred with catechin.
Chen
Chang-Yu
CY
Department of Medical Technology, Foo Yin Institute of Technology, Kaoshiung, Taiwan, Republic of China. ccy1689@ms32.hinet.net
Su
Yuh-Juan
YJ
Wu
Pey-Fen
PF
Shyu
Mei-Miin
MM
D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D017665:Hydroxyl Radical / Q000032:analysis*; D015227:Lipid Peroxidation; D008297:Male; D009532:Nickel / Q000009:adverse effects*; D010949:Plasma / Q000737:chemistry; D024502:alpha-Tocopherol / Q000097:blood*
D000975:Antioxidants; D017665:Hydroxyl Radical; D009532:Nickel; D024502:alpha-Tocopherol