title: Centrosomal abnormalities, multipolar mitoses, and chromosomal instability in head and neck tumours with dysfunctional telomeres.
abstract: Carcinomas of the head and neck typically exhibit complex chromosome aberrations but the underlying mutational mechanisms remain obscure. Evaluation of cell division dynamics in low-passage cell lines from three benign and five malignant head and neck tumours revealed a strong positive correlation between multipolarity of the mitotic spindle and the formation of bridges at anaphase in both benign and malignant tumours. Cells exhibiting a high rate of mitotic abnormalities also showed several chromosome termini lacking TTAGGG repeats and a high frequency of dicentric chromosomes. Multicolour karyotyping demonstrated a preferential involvement in structural rearrangements of chromosomes with deficient telomeres. The majority of malignant, mitotically unstable tumours expressed the reverse transcriptase subunit of telomerase. These data indicate that some of the genomic instability in head and neck tumours is initiated by telomere dysfunction, leading to the formation of dicentric chromosomes. These form chromosome bridges at mitosis that could prevent the normal anaphase-telophase transition. In turn, this may cause an accumulation of centrosomes and mitotic multipolarity. Telomerase expression does not confer total stability to the tumour genome but could be crucial for moderating the rate of chromosomal evolution.
authors.affiliation: Department of Clinical Genetics, University Hospital, SE-221 85 Lund, Sweden. david.gisselsson@klingen.lu.se
authors.forename: D
authors.initials: D
authors.lastname: Gisselsson
authors.forename: T
authors.initials: T
authors.lastname: Jonson
authors.forename: C
authors.initials: C
authors.lastname: Yu
authors.forename: C
authors.initials: C
authors.lastname: Martins
authors.forename: N
authors.initials: N
authors.lastname: Mandahl
authors.forename: J
authors.initials: J
authors.lastname: Wiegant
authors.forename: Y
authors.initials: Y
authors.lastname: Jin
authors.forename: F
authors.initials: F
authors.lastname: Mertens
authors.forename: C
authors.initials: C
authors.lastname: Jin
mesh_terms: D008949:Adenoma, Pleomorphic / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure*; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure*; D018385:Centrosome / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000032:analysis*; D004268:DNA-Binding Proteins; D005260:Female; D006258:Head and Neck Neoplasms / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure*; D006801:Humans; D007621:Karyotyping; D008297:Male; D008938:Mitosis; D009363:Neoplasm Proteins / Q000032:analysis; D010307:Parotid Neoplasms / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure*; D012091:Repetitive Sequences, Nucleic Acid; D019098:Telomerase / Q000032:analysis; D016615:Telomere / Q000737:chemistry*
chemical_list: D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D019098:Telomerase