title: Genetic heterogeneity among bovine leukemia virus genotypes and its relation to humoral responses in hosts.
abstract: The existence of bovine leukemia virus (BLV) genotypes was investigated by restriction fragment length polymorphism (RFLP) analysis using bovine peripheral blood leukocytes collected from different geographical areas of Japan. For this purpose a nested polymerase chain reaction (PCR) for a 444 bp fragment of the envelope (env) gene was used because it was previously reported that this region might be responsible for the serological status in the host. The PCR products from 60 samples of BLV-infected cells were digested with endonucleases BamH I, Bgl I, Bcl I, Hae III and Pvu II. RFLP analysis demonstrated that there were six different genotypes of BLV present among cattle in Japan. In some herds PCR-positive animals were infected with only one genotype, but in other herds a few genotypes were found. One genotype was dominant throughout infected cattle and it was also detected in neoplastic cells from three of four animals with lymphosarcoma and three cell lines persistently infected with BLV. Production of antibodies to BLV in each cattle was surveyed by agar gel immunodiffusion and indirect hemagglutination tests, and the results were compared with those obtained from PCR. No genotype related to decreased immunoreactivity was detected. The difference in anti-viral immune responses of each animal appears to be related to the infection stage and other host factors, not to genetic heterogeneity of the envelope gene.
authors.affiliation: National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki, Japan.
authors.forename: Maria
authors.initials: M
authors.lastname: Licursi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Inoshima
authors.forename: Donglai
authors.initials: D
authors.lastname: Wu
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Ester Teresa
authors.initials: ET
authors.lastname: González
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sentsui
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000502:physiology*; D001483:Base Sequence; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000517:prevention & control; D018740:Genetic Heterogeneity; D005838:Genotype; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D007962:Leukocytes / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins