Molecular cloning of wound inducible transcript (wit 3.0) differentially expressed in edentulous oral mucosa undergoing tooth extraction wound-healing.
Tooth extraction is the most commonly prescribed ablation surgery in dentistry and results in the formation of edentulous mucosa. Although the edentulous mucosa serves as the critical interfacial tissue for removable and implant-assisted prostheses, the structure and physiology of this wound-induced tissue are largely uninvestigated. We addressed the hypothesis that tooth extraction activates the expression of a unique set of genes in healing edentulous mucosa. Using the Differential Display Polymerase Chain Reaction and 5' Rapid Amplification of cDNA Ends protocols, we isolated overlapping cDNAs encoding a 3.0-kb-long mRNA, Wound Inducible Transcript, 3.0 (wit 3.0). In situ hybridization demonstrated that wit 3.0 was primarily expressed by the fibroblasts associated with tooth extraction wound-healing. Appearing to generate from the wit 3.0 gene, two alternative transcripts presented, encoding 215-(wit 3.0 alpha) and 253-(wit 3.0 beta) amino-acid-long peptides with the characteristics of an intracellular molecule. Analysis of these data may provide new clues to the molecular mechanism of edentulous mucosa formation.
Sukotjo
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Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Box 951668, CHS B3-087, 90095-1668, USA.
Abanmy
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AA
Ogawa
T
T
Nishimura
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I
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D006133:Growth Substances / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009061:Mouth Mucosa / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012343:RNA, Transfer / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020539:Sequence Analysis, Protein; D014081:Tooth Extraction; D014158:Transcription, Genetic; D014945:Wound Healing / Q000235:genetics* / Q000502:physiology
D006133:Growth Substances; D010455:Peptides; D011506:Proteins; C460419:wit 3.0 protein, human; D012343:RNA, Transfer