title: Intranasal fluticasone propionate inhibits allergen induced bone marrow eosinophilia in mice.
abstract: Local corticosteroids are currently the most efficient safe anti-allergic treatment, which attenuate eosinophilic tissue inflammation through several mechanisms. We evaluated the effect of local airways corticosteroid on repeated allergen exposure-induced bone marrow activation and airway eosinophilia using the number of eosinophils in bone marrow, bronchoalveolar lavage fluid (BALf) and airways tissue as study end-points. Male BALB/c mice were sensitized by intraperitoneal injections of aluminum-precipitated ovalbumin (OVA) on two different days (5 days apart). Eight days after the second sensitization, the animals were challenged intranasally with OVA or phosphate-buffered saline (PBS) on 5 consecutive days. Concomitantly with challenges mice were treated with fluticasone propionate or respective vehicle. OVA exposures induced a significant increase in eosinophil numbers in bone marrow, BALf and airways tissue (P<0.005). Treatment with fluticasone propionate significantly reduced the increase of absolute number of mature bone marrow eosinophils (P=0.014) and showed a tendency towards decrease in the immature bone marrow eosinophil number (P=0.057) compared to controls. However, fluticasone propionate had no significant effect on BALf and airways tissue eosinophils (P=0.28 and 0.07, respectively). In this murine allergy model intranasal corticosteroid reduced number of bone marrow mature eosinophils, but did not significantly affect airways cell populations.
authors.affiliation: The Lung Pharmacology Group, Department of Respiratory Medicine and Allergology, Institute of Internal Medicine, Göteborg University, Gothenburg, Sweden.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Sergejeva
authors.forename: Masafumi
authors.initials: M
authors.lastname: Tomaki
authors.forename: Teet
authors.initials: T
authors.lastname: Pullerits
authors.forename: Lin-Ling
authors.initials: LL
authors.lastname: Zhao
authors.forename: Malcolm
authors.initials: M
authors.lastname: Johnson
authors.forename: Jan
authors.initials: J
authors.lastname: Lötvall
mesh_terms: D000485:Allergens / Q000009:adverse effects*; D000730:Androstadienes / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism; D004802:Eosinophilia / Q000209:etiology / Q000517:prevention & control*; D000068298:Fluticasone; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000485:Allergens; D000730:Androstadienes; D000893:Anti-Inflammatory Agents; D000068298:Fluticasone