Trypanosoma cruzi I and Trypanosoma cruzi II: recognition of sugar structures by Arachis hypogaea (peanut agglutinin) lectin.
Epimastigote culture forms of different isolates of Trypanosoma cruzi from different mammal hosts, humans, and vectors were tested with FITC-conjugated peanut agglutinin lectin (PNA-FITC). The parasites maintained in axenic medium, liver infusion tryptose. were evaluated by flow cytometric analyses; whereas T. cruzi I (Tcl), which is associated with the sylvatic transmission cycle, was labeled in high percentages with PNA (88-99.2%), T. cruzi II (TcII) (parasites associated with domiciliar cycle) and T. cruzi, zymodeme 3 (Tc/Z3) (also associated with the sylvatic cycle) were labeled in low percentages (TcII, 0-26% and Tc/Z3, 0-12.6%). It was demonstrated that it is possible to differentiate the 2 main T. cruzi subpopulations, TcI and TcII, using Arachis hypogaea. These results also showed a higher variability in TcII in terms of PNA binding.
Araújo
C A C
CA
Department of Protozoology, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Manguinhos, Brazil.
Mello
C B
CB
Jansen
A M
AM
D000818:Animals; D014355:Chagas Disease / Q000469:parasitology*; D005434:Flow Cytometry; D005690:Galactose / Q000032:analysis / Q000378:metabolism*; D019887:Peanut Agglutinin / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000145:classification / Q000378:metabolism*
D019887:Peanut Agglutinin; D005690:Galactose