title: Cloning and sequencing of the serine dehydrogenase gene from Agrobacterium tumefaciens.
abstract: The structural gene for NADP+-dependent serine dehydrogenase [EC 1.1.1.-] from Agrobacterium tumefaciens ICR 1600 was cloned into Escherichia coli cells and its complete DNA sequence was analyzed. The gene encodes a polypeptide containing 249 amino acid residues. The enzyme had high sequence similarity to short-chain alcohol dehydrogenases from bacteria and unknown proteins of Haemophilus influenzae, Escherichia coli, and Saccharomyces cerevisiae.
authors.affiliation: Department of Bioresources Science, Kochi University, Japan.
authors.forename: Hisae
authors.initials: H
authors.lastname: Fujisawa
authors.forename: Shinji
authors.initials: S
authors.lastname: Nagata
authors.forename: Emran Kabir
authors.initials: EK
authors.lastname: Chowdhury
authors.forename: Miki
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Haruo
authors.initials: H
authors.lastname: Misono
mesh_terms: D016960:Agrobacterium tumefaciens / Q000201:enzymology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D010088:Oxidoreductases