The Sequence Analysis and Expression of cDNA of Human Cardiac Myosin Light Chain 1 and the Preparation of Monoclonal Antibody to the Expressed Product.
The nucleotide sequence of cDNA of ventricular myosin light chain 1 of Chinese patients was analyzed. Two remarkable differences in deduced amino acid sequence were found by comparison with amino acid sequence reported previously by Jackowski. The cDNA was expressed in E.coli and the expressed product was used for production of specific polyclonal and monoclonal antibodies. Using both of the poly- and monoclonal antibodies, as well as the expressed product, diagnosis kits for Acute Myocardial infarction was constructed (China patent application number 98 122066.5). Detailed studies on physiological function of cardiac myosin light chain 1 is under way in our lab.
Huang
Ren-Jian
RJ
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. Gongzx@sunm.shcnc.ac.cn
Peng
Bao-Zhen
BZ
Zhou
Guo-Yin
GY
Gong
Zu-Xun
ZX