title: Cytotoxic constituents from Aquilegia ecalcarata.
abstract: A new alkaloid, 7-hydroxy-4-(5'-hydroxymethylfuran-2'-yl)-2-quinolone (1), and a new nitrile derivative, 3alpha,4beta-dihydroxy-6-oxo-1-cyclohexene-1-acetonitrile (2), together with three known oxoaporphine alkaloids, were isolated from the whole plant of Aquilegia ecalcarata. Their structures were established on the basis of spectral evidence. Their in vitro cytotoxicity against different classes of cancer cell lines, including GLC-82 and HCT were determined. The new alkaloid 1 [IC 50 (GLC-82) 8.8 +/- 0.2 microM, IC 50 (HCT) 10.1 +/- 0.3 microM] and hernandonine ( 3) [IC 50 (GLC-82) 7.6 +/- 0.5 microM, IC 50 (HCT) 8.2 +/- 0.5 microM] exhibited cytotoxicity towards the cancer cell lines.
authors.forename: Si-Bao
authors.initials: SB
authors.lastname: Chen
authors.forename: Guang-Yao
authors.initials: GY
authors.lastname: Gao
authors.forename: Ye-Shi
authors.initials: YS
authors.lastname: Li
authors.forename: Shi-Chun
authors.initials: SC
authors.lastname: Yu
authors.forename: Pei Gen
authors.initials: PG
authors.lastname: Xiao
mesh_terms: D000097:Acetonitriles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D053138:Cyclohexenes; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology; D015363:Quinolones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D029626:Ranunculaceae; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: C465100:3,4-dihydroxy-6-oxo-1-cyclohexene-1-acetonitrile; C465099:7-hydroxy-4-(5'-hydroxymethylfuran-2'-yl)-2-quinolone; D000097:Acetonitriles; D000470:Alkaloids; D053138:Cyclohexenes; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D015363:Quinolones; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue