title: IgG antibody subclass response against equine herpesvirus type 4 in horses.
abstract: In this study, IgG subclass responses against equine herpesvirus type 4 (EHV-4) were examined by enzyme-linked immunosorbent assay (ELISA) using a type-specific region of EHV-4 glycoprotein G (gG). ELISA using sera collected from horses experimentally infected with EHV-4 revealed that IgGa and IgGb antibodies were detected at high level, but IgGc and IgG(T) antibody responses were detected at low level or were undetectable. The IgGa antibody response reached its peak on day 10 post-infection, and then dropped. The IgGb antibody response reached its maximum level on day 12 post-infection, and then the level was sustained during at least 28 days after infection. Forty healthy racehorses that had already been infected with EHV-4 possessed antibody against EHV-4. Although IgGa antibodies specific for EHV-4 were not detected in any horses, IgGb antibodies were detected and the levels correlated with total IgG antibodies against EHV-4 gG. The results suggest that EHV-4-specific IgGa and IgGb antibodies are induced in EHV-4-infected horses, and that IgGb antibody, but not IgGa, is long lasting.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Mizukoshi
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
authors.forename: Masataka
authors.initials: M
authors.lastname: Hamano
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Iwata
authors.forename: Tomio
authors.initials: T
authors.lastname: Matsumura
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Takeo
authors.initials: T
authors.lastname: Sugiura
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D023141:Herpesvirus 4, Equid / Q000276:immunology*; D006734:Horse Diseases / Q000276:immunology* / Q000821:virology; D006736:Horses; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood* / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes