Gudden's dorsal tegmental nucleus is activated in carbachol-induced active (REM) sleep and active wakefulness.
Previous studies have shown that GABAergic processes in the ponto-mesencephalic region of the brainstem are involved in the generation of wakefulness and active sleep (AS). The dorsal and ventral tegmental nuclei of Gudden (DTN and VTN, respectively) are known to contain a large population of GABAergic neurons. In the present study, utilizing Fos immunoreactivity as a marker of neuronal activity, it was determined that GABAergic DTN pars dorsalis neurons are active during active wakefulness and AS induced by carbachol, but not during quiet wakefulness or quiet sleep. In contrast, no differences in the number of Fos immunoreactive neurons were observed in the DTN pars ventralis and VTN across behavioral states.
Torterolo
Pablo
P
Department of Physiology and the Brain Research Institute, UCLA School of Medicine, 90095, USA.
Sampogna
Sharon
S
Morales
Francisco R
FR
Chase
Michael H
MH
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002415:Cats; D018679:Cholinergic Agonists / Q000494:pharmacology; D007150:Immunohistochemistry; D009434:Neural Pathways / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000187:drug effects / Q000378:metabolism; D012890:Sleep / Q000187:drug effects / Q000502:physiology; D012895:Sleep, REM / Q000187:drug effects / Q000502:physiology*; D013681:Tegmentum Mesencephali / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014851:Wakefulness / Q000187:drug effects / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D018679:Cholinergic Agonists; D016760:Proto-Oncogene Proteins c-fos; D005680:gamma-Aminobutyric Acid; D002217:Carbachol