title: Expression of monocyte chemoattractant protein-1 in proximal tubular epithelial cells in a rat model of progressive kidney failure.
abstract: Impairment of kidney function in various types of glomerular disease is associated with tubulointerstitial changes. Monocyte chemoattractant protein-1 (MCP-1) is up-regulated in the tubulointerstitium and in the glomeruli in many human and experimental kidney disorders. We investigated the localization of MCP-1 expression in a rat model of progressive kidney failure. Male Wistar rats were subjected to subtotal nephrectomy (n = 30) or sham surgery (n = 30). Immunohistochemistry with immunoelectron microscopy and in situ hybridization were used to examine the expression of MCP-1 protein and messenger ribonucleic acid (mRNA) in the kidney, respectively. MCP-1 protein and mRNA were hardly detected in both glomeruli and tubulointerstitium of control rats. However, in the rats subjected to nephrectomy, MCP-1 expression was increased in the tubular cells of the remnant kidney, accompanied by significant macrophage infiltration. MCP-1 was observed mainly in the proximal tubular cells and only weakly in distal tubular cells. No significant expression of MCP-1 protein or mRNA was noted in the glomeruli. Immunoelectron microscopy showed the presence of MCP-1 in the rough endoplasmic reticulum of proximal tubular cells, confirming that MCP-1 is produced in proximal tubular cells. MCP-1 was also observed in endocytic vesicles adjacent to the brush border of proximal tubular cells, suggesting incorporation of MCP-1 from the tubular lumen. Our findings indicate localized expression of MCP-1 in proximal tubular cells in the remnant kidney and suggest that MCP-1 in proximal tubular cells is involved in tubulointerstitial damage in chronic kidney failure associated with glomerular hypertension.
authors.affiliation: Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Yahatanishi, Kitakyushu, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ota
authors.forename: Masahito
authors.initials: M
authors.lastname: Tamura
authors.forename: Akihiko
authors.initials: A
authors.lastname: Osajima
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Doi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kudo
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Anai
authors.forename: Masanobu
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Tomoya
authors.initials: T
authors.lastname: Nishino
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Nakashima
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics*; D004195:Disease Models, Animal; D018450:Disease Progression; D007684:Kidney Tubules / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D009392:Nephrectomy; D051381:Rats; D017208:Rats, Wistar; D051437:Renal Insufficiency / Q000235:genetics* / Q000473:pathology; D019459:Urothelium / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure
chemical_list: D018932:Chemokine CCL2