title: Acute handling stress modulates methylphenidate-induced catecholamine overflow in the medial prefrontal cortex.
abstract: Although stress is an extensively investigated phenomenon, the effects of specific stressors on the pharmacologic activity of routinely administered drugs are less well characterized. We designed the present study to investigate the effect of handling stress on catecholaminergic responsivity following an acute methylphenidate (MP, Ritalin) challenge in the medial prefrontal cortex (mPFC). Norepinephrine (NE) and dopamine (DA) levels were simultaneously measured in 15-min samples of PFC dialysate using HPLC coupled with electrochemical detection. Sprague-Dawley rats were handled for 15 min, which produced an increase from basal extracellular DA and NE levels. Handling stress attenuates the DA response when administered 2 h prior to IP MP, whereas handling stress enhances the DA response when administered simultaneously with IG MP. These findings suggest that persistent alterations in mesocorticolimbic DA-ergic activity are induced by a short exposure to restraint stress as evidenced by the altered response to MP challenge.
authors.affiliation: Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Marsteller
authors.forename: Madina R
authors.initials: MR
authors.lastname: Gerasimov
authors.forename: Wynne K
authors.initials: WK
authors.lastname: Schiffer
authors.forename: Justin M
authors.initials: JM
authors.lastname: Geiger
authors.forename: Channing R
authors.initials: CR
authors.lastname: Barnett
authors.forename: Jana
authors.initials: J
authors.lastname: Schaich Borg
authors.forename: Sabria
authors.initials: S
authors.lastname: Scott
authors.forename: Jill
authors.initials: J
authors.lastname: Ceccarelli
authors.forename: Nora D
authors.initials: ND
authors.lastname: Volkow
authors.forename: Patricia E
authors.initials: PE
authors.lastname: Molina
authors.forename: David L
authors.initials: DL
authors.lastname: Alexoff
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Dewey
mesh_terms: D000208:Acute Disease; D018759:Adrenergic Uptake Inhibitors / Q000494:pharmacology*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D004334:Drug Administration Schedule; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D006234:Handling, Psychological; D008297:Male; D008774:Methylphenidate / Q000494:pharmacology*; D017551:Microdialysis; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D009434:Neural Pathways / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D009638:Norepinephrine / Q000378:metabolism; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D017729:Presynaptic Terminals / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000378:metabolism* / Q000503:physiopathology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D017557:Ventral Tegmental Area / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology
chemical_list: D018759:Adrenergic Uptake Inhibitors; D002395:Catecholamines; D008774:Methylphenidate; D004298:Dopamine; D009638:Norepinephrine