Technical-scale production of cyanophycin with recombinant strains of Escherichia coli.
By the use of Escherichia coli DH1 harboring cphA from Synechocystis sp. strain PCC6803, large-scale production of cyanophycin at 30- and 500-liter culture volumes was established. Transcription of cphA was controlled by the thermosensitive cI857 repressor, which enabled induction of cphA by a simple temperature shift in the culture fluid. Maximum cyanophycin cell content of up to 24% (wt/wt) of cellular dry matter was obtained by induction in the early exponential growth phase and cultivation of the cells in terrific broth complex medium. Synthesis of cyanophycin was found to be strongly dependent on the presence of complex components, and in mineral salts medium the cells synthesized and accumulated cyanophycin only if Casamino Acids were added. Cultivations were done at the 500-liter scale, allowing the provision of cell mass for the preparation of cyanophycin at the kilogram scale. Isolation of cyanophycin was achieved by a new acid extraction procedure which allowed large-scale purification of the polyamide from whole cells.
Frey
Kay M
KM
Institut für Mikrobiologie, Westfälische Wilhelms-Universität, Corrensstrasse 3, D-48149 Münster, Germany.
Oppermann-Sanio
Fred B
FB
Schmidt
Holger
H
Steinbüchel
Alexander
A
D001426:Bacterial Proteins; D003470:Culture Media; D000458:Cyanobacteria / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D011995:Recombination, Genetic; D014169:Transformation, Bacterial; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D003470:Culture Media; D010940:Plant Proteins; C024250:cyanophycin; D001618:beta-Lactamases