Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein.
Smad4 is a novel tumor suppressor gene which is mutated or deleted in about 50% of pancreatic carcinoma and 30% of colon cancer. SMAD4 was expressed in Pichia pastoris and was characterized. The molecular weight of the expression product was shown to be about 67 kD, and 13 amino acids in N terminal were determined and were identical with the putative sequence from Smad4 cDNA, and it was able to specifically react with the antibody against SMAD4.
Liu
Yi-Ping
YP
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. huangc@nic.bmi.ac.cn
Yang
Xiao
X
Xu
Xiao-Ling
XL
Deng
Ji-Xian
JX
Cheng
Xuan
X
Huang
Cui-Fen
CF