Characterization of two non-homoeologous nuclear genes encoding mitochondrial alternative oxidase in common wheat.
Mitochondrial alternative oxidase (AOX) is the terminal oxidase responsible for cyanide-insensitive and salicylhydroxamic acid-sensitive respiration. We have isolated two non-homoeologous genes (Waox1a and Waox1c) encoding AOX proteins from common wheat (Triticum aestivum L.). These two genes were orthologous to rice AOX1a and AOX1c, and their exon/intron structure was conserved, as it is in most other plant AOX genes. Southern blot analysis indicated that both Waox1a and Waox1c were located in at least three homoeologous loci and that additional AOX genes with lower homology were present in the genome of common wheat. The Waox1a and Waox1c loci were respectively assigned to the homoeologous group 2 and 6 chromosomes. The steady-state level of Waox1a and Waox1c transcripts increased under cold stress, while only that of Waox1a was increased by cyanide treatment.
Takumi
Shigeo
S
Faculty of Agriculture, and Division of Life Science, The Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe, Japan.
Tomioka
Miki
M
Eto
Kayou
K
Naydenov
Nayden
N
Nakamura
Chiharu
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics*; D032461:Chromosomes, Plant; D003080:Cold Temperature; D018744:DNA, Plant / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D018745:Genome, Plant; D007438:Introns; D008928:Mitochondria / Q000201:enzymology* / Q000235:genetics; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011003:Ploidies; D011190:Potassium Cyanide / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology; D014908:Triticum / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D000099188:Alternative Oxidase
D018744:DNA, Plant; D004791:Enzyme Inhibitors; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D011190:Potassium Cyanide; D000099188:Alternative Oxidase