title: PUVA-induced apoptosis involves mitochondrial dysfunction caused by the opening of the permeability transition pore.
abstract: The mechanism of cell death was investigated in Jurkat cells exposed to the combination of psoralen and UVA irradiation (PUVA). Apoptosis was by far prevailing over necrosis and involved mitochondrial dysfunction. The collapse of mitochondrial membrane potential, appears to be caused by the opening of the mitochondrial permeability transition pore since its inhibitor, cyclosporin A, prevented mitochondrial dysfunction and largely attenuated apoptosis. Apoptosis also occurred in cells treated with the photoproducts generated by irradiating psoralen in vitro with an oxygen-dependent process. Thus, the involvement of reactive oxygen species in the onset of PUVA-induced apoptosis appears mostly related to psoralen photooxidation.
authors.affiliation: Dipartimento di Chimica Biologica, Università di Padova, Viale Giuseppe Colombo 3, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Canton
authors.forename: Sergio
authors.initials: S
authors.lastname: Caffieri
authors.forename: Francesco
authors.initials: F
authors.lastname: Dall'Acqua
authors.forename: Fabio
authors.initials: F
authors.lastname: Di Lisa
mesh_terms: D017209:Apoptosis; D005363:Ficusin / Q000494:pharmacology*; D006801:Humans; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D007473:Ion Channels / Q000037:antagonists & inhibitors*; D019169:Jurkat Cells; D008564:Membrane Potentials / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D007473:Ion Channels; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D017319:Photosensitizing Agents; D011065:Poly(ADP-ribose) Polymerases; D005363:Ficusin