Method for purification of fluorescence-labeled oligosaccharides by pyridylamination.
We developed a convenient method for purification of PA-oligosaccharides to remove contaminants originating from natural fluorescent materials, and excess reagents as well as by-products of tagging reactions in glycan analysis. The method, using a C18-cartridge, is simple and powerful to remove them. Several examples of experiments that showed the usefulness of this purification method are described in this report.
Natsuka
Shunji
S
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Japan. natsuka@ipc.kit.ac.jp
Adachi
Jiro
J
Kawaguchi
Masahumi
M
Ichikawa
Akira
A
Ikura
Koji
K
D000588:Amines / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D011725:Pyridines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000588:Amines; D005456:Fluorescent Dyes; D009844:Oligosaccharides; D011725:Pyridines; C023666:pyridine