title: Redox status-dependent regulation of cyclooxygenases mediates the capsaicin-induced apoptosis in human neuroblastoma cells.
abstract: Cyclooxygenases (COX) appear to be involved in the mechanism of apoptosis in various cancer cells. In this study we investigated the role of COX in the capsaicin (Cap)-induced apoptosis in SK-N-SH human neuroblastoma cells. Cap induced decreased cell viability and apoptosis in a dose-dependent manner. Cap also significantly reduced the basal generation of reactive oxygen species (ROS) and lipid peroxidation in a time-dependent fashion. Cap markedly suppressed the expression of COX-1 and COX-2. Pretreatment with NS-398, a selective COX-2 inhibitor, or indomethacin, a nonselective COX inhibitor, significantly enhanced the Cap-induced decreased cell viability and apoptosis. Exogenous application of an oxidant, H2O2, significantly prevented the Cap-induced apoptosis and suppressed the expression of COX isoforms. These results suggest that redox status-dependent regulation of COX expression may mediate apoptosis induced by Cap in human neuroblastoma cells.
authors.affiliation: Department of Physiology, College of Medicine, Kwandong University, Kangnung, Korea.
authors.forename: Yong Soo
authors.initials: YS
authors.lastname: Lee
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Kwon
authors.forename: Da Qing
authors.initials: DQ
authors.lastname: Jin
authors.forename: Seung Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Young Shin
authors.initials: YS
authors.lastname: Kang
authors.forename: Keun
authors.initials: K
authors.lastname: Huh
authors.forename: Jung-Ae
authors.initials: JA
authors.lastname: Kim
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D002211:Capsaicin / Q000494:pharmacology*; D002470:Cell Survival; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis* / Q000494:pharmacology; D008565:Membrane Proteins; D009447:Neuroblastoma / Q000473:pathology*; D016877:Oxidants; D010084:Oxidation-Reduction; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D007527:Isoenzymes; D008565:Membrane Proteins; D016877:Oxidants; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D002211:Capsaicin