title: A simple, mechanistic model for directional instability during mitotic chromosome movements.
abstract: During mitosis, chromosomes become attached to microtubules that emanate from the two spindle poles. Thereafter, a chromosome moves along these microtubule "tracks" as it executes a series of movements that bring it to the spindle equator. After the onset of anaphase, the sister chromatids separate and move to opposite spindle poles. These movements are often characterized by "directional instability" (a series of runs with approximately constant speed, punctuated by sudden reversals in the direction of movement). To understand mitosis, it is critical to describe the physical mechanisms that underlie the coordination of the forces that drive directional instability. We propose a simple mechanistic model that describes the origin of the forces that move chromosomes and the coordination of these forces to produce directional instability. The model demonstrates that forces, speeds, and direction of motion associated with prometaphase through anaphase chromosome movements can be predicted from the molecular kinetics of interactions between dynamic microtubules and arrays of microtubule binding sites that are linked to the chromosome by compliant elements.
authors.affiliation: Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA. ajitj@umich.edu
authors.forename: Ajit P
authors.initials: AP
authors.lastname: Joglekar
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Hunt
mesh_terms: D000705:Anaphase; D001665:Binding Sites; D055592:Biophysical Phenomena; D001703:Biophysics; D002875:Chromosomes / Q000648:ultrastructure*; D019281:Dimerization; D007700:Kinetics; D018386:Kinetochores; D008938:Mitosis; D008956:Models, Chemical; D013997:Time Factors; D014404:Tubulin / Q000737:chemistry
chemical_list: D014404:Tubulin