Regulation on maturation and function of dendritic cells by Ganoderma lucidum polysaccharides.
Ganoderma lucidum polysaccharides (Gl-PS) exhibits a variety of immunomodulatory activities, and dendritic cells (DC) are professional antigen presenting cells, which are pivotal for initiation of primary immune response. In this study, the regulatory effects of Gl-PS on maturation and function of cultured murine bone marrow derived DC were investigated in vitro. Gl-PS (0.8, 3.2, or 12.8 microg/ml) could increase the co-expression of CD11c and I-A/I-E molecules on DC surface, promote mRNA expression of cytokine IL-12 p40 in DC and augment protein production of IL-12 P40 in culture supernatants. The lymphocyte proliferation of mixed lymphocyte culture (MLC) induced by mature DC was enhanced by Gl-PS, either. Gl-PS was shown to promote not only the maturation of cultured murine bone marrow derived DC in vitro, but also the immune response initiation induced by DC.
Cao
Li-Zhen
LZ
Department of Pharmacology, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100083, People's Republic of China.
Lin
Zhi-Bin
ZB
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D039521:CD11c Antigen / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D018664:Interleukin-12 / Q000235:genetics; D053762:Interleukin-12 Subunit p40; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D011134:Polysaccharides / Q000494:pharmacology*; D021122:Protein Subunits / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020940:Reishi; D020133:Reverse Transcriptase Polymerase Chain Reaction
D039521:CD11c Antigen; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D053762:Interleukin-12 Subunit p40; D011134:Polysaccharides; D021122:Protein Subunits; D012333:RNA, Messenger; D018664:Interleukin-12