title: Systematic identification of pathways that couple cell growth and division in yeast.
abstract: Size homeostasis in budding yeast requires that cells grow to a critical size before commitment to division in the late prereplicative growth phase of the cell cycle, an event termed Start. We determined cell size distributions for the complete set of approximately 6000 Saccharomyces cerevisiae gene deletion strains and identified approximately 500 abnormally small (whi) or large (lge) mutants. Genetic analysis revealed a complex network of newly found factors that govern critical cell size at Start, the most potent of which were Sfp1, Sch9, Cdh1, Prs3, and Whi5. Ribosome biogenesis is intimately linked to cell size through Sfp1, a transcription factor that controls the expression of at least 60 genes implicated in ribosome assembly. Cell growth and division appear to be coupled by multiple conserved mechanisms.
authors.affiliation: Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8.
authors.forename: Paul
authors.initials: P
authors.lastname: Jorgensen
authors.forename: Joy L
authors.initials: JL
authors.lastname: Nishikawa
authors.forename: Bobby-Joe
authors.initials: BJ
authors.lastname: Breitkreutz
authors.forename: Mike
authors.initials: M
authors.lastname: Tyers
mesh_terms: D064200:Cdh1 Proteins; D002453:Cell Cycle; D002455:Cell Division; D002466:Cell Nucleolus / Q000378:metabolism; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D004843:Epistasis, Genetic; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D020043:Genes, Essential; D005800:Genes, Fungal; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010101:Oxygen Consumption; D010641:Phenotype; D011494:Protein Kinases / Q000235:genetics / Q000502:physiology; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
chemical_list: C577583:CDH1 protein, S cerevisiae; D064200:Cdh1 Proteins; D004268:DNA-Binding Proteins; C117421:SFP1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C071569:SCH9 protein kinase