Inhibitory effect of sulfur dioxide and other stress compounds in wine on the ATPase activity of Oenococcus oeni.
Malolactic fermentation (MLF) is carried out by Oenococcus oeni under very harsh conditions. This paper shows that stress compounds in wine such as SO(2), fatty acids and copper have an inhibitory effect on cell growth and MLF duration, and relates this effect to an inhibition of ATPase activity. Of the stress compounds, SO(2) and dodecanoic acid had the strongest effect, decreasing the ATPase specific activity to 37% and 58%, respectively. It can be concluded that ATPase is a good indicator of the physiological state of the cells and their ability to lead MLF.
Carreté
Ramon
R
Departament de Bioquímica i Biotecnologia, Unitat d'Enologia del CeRTA, Universitat Rovira i Virgili, Plaça Imperial Tàrraco, 1, 43005 Tarragona, Catalonia, Spain.
Vidal
M Teresa
MT
Bordons
Albert
A
Constantí
Magda
M
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology; D005285:Fermentation; D006095:Gram-Positive Cocci / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D007934:Leuconostoc / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D008291:Malate Dehydrogenase / Q000378:metabolism; D010575:Pesticides / Q000494:pharmacology; D013458:Sulfur Dioxide / Q000494:pharmacology*; D014920:Wine / Q000382:microbiology*
D004791:Enzyme Inhibitors; D005227:Fatty Acids; D010575:Pesticides; D013458:Sulfur Dioxide; C037731:malolactic enzyme; D008291:Malate Dehydrogenase; D000251:Adenosine Triphosphatases