High Expression of Human Persephin in Insect Cells.
The human persephin (PSP) was expressed in Tn-5B1-4 insect cells using the Bac to Bac baculovirus expression system. The expressed product amounted for 20% of total cellular soluble proteins. The expressed product was purified by Ni(2+) affinity chromatography and the activity assays showed that it could significantly prolong the survival of spinal cord neurons.
Chen
Zhe-Yu
ZY
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. xfwu@sunm.shcnc.ac.cn
He
Zhi-Yong
ZY
He
Cheng
C
Lu
Chang-Lin
CL
Wu
Xiang-Fu
XF