LC determination of YM466, a new factor Xa inhibitor, in rat and dog plasma.
A specific method for the determination of YM466, a novel Factor Xa inhibitor, in rat and dog plasma was developed and validated. YM466 was extracted from plasma by solid-phase extraction and analyzed by UV-HPLC at an absorbance wavelength of 240 nm. The intra-day precision and accuracy ranged from 0.8 to 2.4% and 0.1 to 5.0% in rats, and from 1.6 to 2.4% and 0.0 to 4.1% in dogs, respectively. The lower limit of quantification was 10 ng/ml when 1 ml of plasma was used. No endogenous interference was observed in the plasma of rats and dogs.
Mano
Yuji
Y
Drug Metabolism Laboratories, Yamanouchi Pharmaceutical Co., Ltd., 1-8 Azusawa 1-Chome, Itabashi-ku, 174-8511, Tokyo, Japan. mano@yamanuchi.co.jp
Aoki
Toshiko
T
Kikuchi
Yumiko
Y
Soeishi
Yoshiaki
Y
Usui
Takashi
T
Kamimura
Hidetaka
H
D000818:Animals; D000925:Anticoagulants / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004355:Drug Stability; D065427:Factor Xa Inhibitors; D005615:Freezing; D008297:Male; D009281:Naphthalenes / Q000097:blood*; D010880:Piperidines / Q000097:blood*; D051381:Rats; D011916:Rats, Inbred F344; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000925:Anticoagulants; D065427:Factor Xa Inhibitors; D009281:Naphthalenes; D010880:Piperidines; C111032:YM 60828