title: 1H NMR study of the binding of Bis(acridines) to d(AT)5.d(AT)5. 1. Mode of binding.
abstract: 1H NMR has been used to investigate the mode of binding to d(AT)5.d(AT)5 of a series of bis(acridine) derivatives connected by different types of linker chains. The length and character (ionic, aliphatic, rigid, and flexible) of the linker chains are found to have a profound effect on the binding of these derivatives to the DNA. Bis(acridine) derivatives with linker chains shorter than 9 A monointercalate under the conditions used in the NMR study, whereas those bis(acridines) with chains of 9.8 A or longer bisintercalate. We find no evidence for the violation of the so-called neighbor exclusion principle. Although all of the bis(acridines) contain the same chromophores, their NMR spectra clearly demonstrate that they form complexes with d(AT)5.d(AT)5 which have different structures. This emphasizes the important effect that the linker chain has on the structure of the intercalation complex.
authors.affiliation: Department of Chemistry, University of California, San Diego, La Jolla, California 92093, USA.
authors.forename: N
authors.initials: N
authors.lastname: Assa-Munt
authors.forename: W A
authors.initials: WA
authors.lastname: Denny
authors.forename: W
authors.initials: W
authors.lastname: Leupin
authors.forename: D R
authors.initials: DR
authors.lastname: Kearns
mesh_terms: D000166:Acridines / Q000737:chemistry*; D000227:Adenine Nucleotides / Q000737:chemistry; D000585:Aminacrine / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013942:Thymine Nucleotides / Q000737:chemistry
chemical_list: D000166:Acridines; D000227:Adenine Nucleotides; D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D013942:Thymine Nucleotides; D000585:Aminacrine