Influence of the period-dependent circadian clock on diurnal, circadian, and aperiodic gene expression in Drosophila melanogaster.
We measured daily gene expression in heads of control and period mutant Drosophila by using oligonucleotide microarrays. In control flies, 72 genes showed diurnal rhythms in light-dark cycles; 22 of these also oscillated in free-running conditions. The period gene significantly influenced the expression levels of over 600 nonoscillating transcripts. Expression levels of several hundred genes also differed significantly between control flies kept in light-dark versus constant darkness but differed minimally between per(01) flies kept in the same two conditions. Thus, the period-dependent circadian clock regulates only a limited set of rhythmically expressed transcripts. Unexpectedly, period regulates basal and light-regulated gene expression to a very broad extent.
Lin
Yiing
Y
Department of Genetics, Washington University Medical School, St. Louis, MO, 63110, USA.
Han
Mei
M
Shimada
Brian
B
Wang
Lin
L
Gibler
Therese M
TM
Amarakone
Aloka
A
Awad
Tarif A
TA
Stormo
Gary D
GD
Van Gelder
Russell N
RN
Taghert
Paul H
PH
D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology* / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D020869:Gene Expression Profiling; D017344:Genes, Insect / Q000528:radiation effects; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D056950:Period Circadian Proteins; D017440:Photoperiod
D029721:Drosophila Proteins; D009687:Nuclear Proteins; C539040:PER protein, Drosophila; D056950:Period Circadian Proteins