Immortalization of normal adult human middle ear epithelial cells using a retrovirus containing the E6/E7 genes of human papillomavirus type 16.
A human middle ear epithelial cell line (HMEEC-1) was established using human papillomavirus E6/E7 genes. HMEEC-1 has remained morphologically and phenotypically stable, even after 50 passages. The cells are anchorage-dependent and nontumorigenic when injected into nude mice. This cell line thus provides a new tool for the study of normal cell biology and the pathological processes associated with the epithelial cells of the middle ear in otitis media. HMEEC-1 will also be useful in the search for new drugs and biological agents for the treatment of otitis media.
Chun
Young-Myoung
YM
Gonda Department of Cell and Molecular Biology, Los Angeles, California, USA.
Moon
Sung-Kyun
SK
Lee
Haa-Yung
HY
Webster
Paul
P
Brackmann
Derald E
DE
Rhim
Johng S
JS
Lim
David J
DJ
D000328:Adult; D000818:Animals; D002460:Cell Line; D004432:Ear, Middle / Q000166:cytology*; D004847:Epithelial Cells; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction
C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16