Flavonoids uptake and their effect on cell cycle of human colon adenocarcinoma cells (Caco2).
Green tea, mainly through its constituents epigallocatechin gallate, epigallocatechin, epicatechin gallate and epicatechin, has demonstrated anticarcinogenic activity in several animal models, including those for skin, lung and gastro-intestinal tract cancer, although less is known about colorectal cancer. Quercetin, the major flavonoid present in vegetables and fruit, exerts potential anticarcinogenic effects in animal models and cell cultures, but less is known about quercetin glucosides. The objectives of this study were to investigate (i) the antioxidant activity of the phenolic compounds epicatechin, epigallocatechin gallate, gallic acid and quercetin-3-glucoside; (ii) the cytotoxicity of different concentrations of epicatechin, epigallocatechin gallate, and gallic acid; (iii) the cellular uptake of epicatechin, epigallocatechin gallate, gallic acid and quercetin-3-glucoside and (iv) their effect on the cell cycle. Human colon adenocarcinoma cells were used as experimental model. The results of this study indicate that all dietary flavonoids studied (epicatechin, epigallocatechin gallate, gallic acid and quercetin-3-glucoside) show a significant antioxidant effect in a chemical model system, but only epigallocatechin gallate or gallic acid are able to interfere with the cell cycle in Caco2 cell lines. These data suggest that the antioxidant activity of flavonoids is not related to the inhibition of cellular growth. From a structural point of view, the galloyl moiety appears to be required for both the antioxidant and the antiproliferative effects.
Salucci
M
M
National Institute of Food and Nutrition Research, Via Ardeatina 546, 00178, Rome, Italy. salucci@inran.it
Stivala
L A
LA
Maiani
G
G
Bugianesi
R
R
Vannini
V
V
D000230:Adenocarcinoma / Q000473:pathology*; D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology / Q000633:toxicity; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology / Q000633:toxicity; D002392:Catechin / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology / Q000633:toxicity; D005707:Gallic Acid / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D015394:Molecular Structure; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity; D013329:Structure-Activity Relationship; D013662:Tea / Q000737:chemistry; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D005419:Flavonoids; D013662:Tea; C016527:isoquercitrin; D005707:Gallic Acid; D002392:Catechin; D011794:Quercetin; D006861:Hydrogen Peroxide; C045651:epigallocatechin gallate