Temporal and spatial variations in kinetics of alkaline phosphatase in sediments of a shallow Chinese eutrophic lake (Lake Donghu).
Monthly sediment and interstitial water samples were collected in a shallow Chinese freshwater lake (Lake Donghu) from three areas to determine if alkaline phosphatase activity (APA) plays an important role, in phosphorus cycling in sediment. The seasonal variability in the kinetics of APA and other relevant parameters were investigated from 1995-1996. The phosphatase hydrolyzable phosphorus (PHP) fluctuated seasonally in interstitial water, peaking in the spring. A synchronous pattern was observed in chlorophyll a contents in surface water in general. The orthophosphate (o-P) concentrations in the interstitial water increased during the spring. An expected negative relationship between PHP and Vmax of APA is not evident in interstitial water. The most striking feature of the two variables is their co-occurring, which can be explained in terms of an induction mechanism. It is argued that phosphatase activity mainly contributes to the driving force of o-P regeneration from PHP in interstitial water, supporting the development of phytoplankton biomass in spring. The Vmax values in sediment increased during the summer, in conjunction with lower Km values in interstitial water that suggest a higher affinity for the substrate. The accumulation of organic matter in the sediment could be traced back to the breakdown of the algal spring bloom, which may stimulate APA with higher kinetic efficiency, by a combination of the higher Vmax in sediments plus lower Km values in interstitial water, in summer. In summary, a focus on phosphatase and its substrate in annual scale may provide a useful framework for the development of novel P cycling, possible explanations for the absence of a clear relationship between PHP and APA were PHP released from the sediment which induced APA, and the presence of kinetically higher APA both in sediment and interstitial water which permitted summer mineralization of organic matter derived from the spring bloom to occur. The study highlighted the need for distinguishing functionally distinct extracellular enzymes between the sediment and interstitial water of lakes.
Yiyong
Zhou
Z
Lake Donghu Ecosystem Experimental Station, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan. zhouyy@ihb.ac.cn
Jianqiu
Li
L
Min
Zhang
Z
D000469:Alkaline Phosphatase / Q000494:pharmacology*; D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D005068:Eutrophication; D019015:Geologic Sediments / Q000737:chemistry* / Q000382:microbiology; D007700:Kinetics; D009930:Organic Chemicals / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D012621:Seasons; D014873:Water Pollutants
D009930:Organic Chemicals; D014873:Water Pollutants; D002734:Chlorophyll; D010758:Phosphorus; D000469:Alkaline Phosphatase; D000077194:Chlorophyll A