[A method for analysis of biochemical phase of anti-HIV antibody and HIV antigen reaction by release of Ag+-sensitive-SH-containing nonprotein compounds].
The phenomenon of release of Ag(+)-sensitive SH containing protein compounds during reaction of serum antibodies to HIV with HIV antigen glycoproteins (AgHIV) (both reagents taken from diagnostic kits) is described. These compounds were detected in nonprotein fractions of serum anti-HIV + AgHIV reaction media and disappeared after dialysis of nonprotein fractions. On the other hand, this phenomenon was not detected during addition of AgHIV to the serum containing no AgHIV. Presumably, the phenomenon should be referred to secondary concomitant reaction, which seems to be mediated by conformational and/or other processes only during highly specific antigen-antibody intermolecular interactions. That is why this phenomenon seems to characterize the biochemical phase of immune reactions, which can be associated with the denaturing processes as well, running in serum anti-HIV + AgHIV reaction media, and it can be used as an additional test in evaluating anti-HIV antibodies.
Kostiushov
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D000937:Antigen-Antibody Reactions; D019411:Clinical Laboratory Techniques; D015483:HIV Antibodies / Q000737:chemistry / Q000378:metabolism*; D015488:HIV Antigens / Q000737:chemistry / Q000378:metabolism*; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D012834:Silver; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents
D015483:HIV Antibodies; D015488:HIV Antigens; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D012834:Silver