title: The ADAM1a and ADAM1b genes, instead of the ADAM1 (fertilin alpha) gene, are localized on mouse chromosome 5.
abstract: Fertilin is reported to be a heterodimeric protein composed of A Disintegrin And Metalloprotease 1 (ADAM1, fertilin alpha) and ADAM2 (fertilin beta) located on the sperm surface. In the process of clarifying the molecular basis of mouse ADAM1, we have identified two intron-less mouse genes encoding different isoforms of ADAM1, termed ADAM1a and ADAM1b. The amino acid sequences of ADAM1a and ADAM1b deduced from the DNA sequences were homologous to each other (99% identity) in the pro- and metalloprotease domains, whereas the C-terminal half region of ADAM1a, including the disintegrin and Cys-rich domains, shared only a low degree of identity (37%) with that of ADAM1b. These two genes were both localized on mouse chromosome 5 as a single copy gene, and were expressed specifically in the testis. These data demonstrate the presence of the ADAM1a (Adam1a) and ADAM1b (Adam1b) genes in mouse, instead of the ADAM1 gene, and may imply different roles of ADAM1a and ADAM1b in spermatogenesis, sperm maturation, and/or fertilization.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nishimura
authors.forename: Ekyune
authors.initials: E
authors.lastname: Kim
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Fujimori
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kashiwabara
authors.forename: Asato
authors.initials: A
authors.lastname: Kuroiwa
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Tadashi
authors.initials: T
authors.lastname: Baba
mesh_terms: D051722:ADAM Proteins; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005260:Female; D000072196:Fertilins; D015870:Gene Expression; D017404:In Situ Hybridization, Fluorescence; D007438:Introns / Q000235:genetics; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008666:Metalloendopeptidases / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D008562:Membrane Glycoproteins; D020033:Protein Isoforms; D012313:RNA; D004247:DNA; D051722:ADAM Proteins; C000606455:Adam1a protein, mouse; C000606457:Adam1b protein, mouse; C000606458:Adam2 protein, mouse; D000072196:Fertilins; D008666:Metalloendopeptidases