title: Blast crisis of Philadelphia chromosome-positive chronic myeloid leukaemia carrying micro-bcr breakpoint (e19a2 and e191a).
abstract: We describe two Philadelphia chromosome-positive chronic myeloid leukaemia (Ph-positive CML) patients carrying micro-bcr (micro-bcr) breakpoint, who developed blast crisis within 5 years of diagnosis. Reverse transcription polymerase chain reaction analysis of bone marrow cells using primers specific for the p210BCR-ABL fusion transcript showed aberrant large-sized bands in both cases (986 bp in patient 1 and 1031 bp in patient 2). Sequencing analysis of these products revealed BCR-ABL fusion transcripts with e19a2 in patient 1 and e191a in patient 2. These findings suggest that CML carrying micro-bcr breakpoint may exhibit a similar clinical course to classical CML, and that it may not be a mild form of the disease.
authors.affiliation: Department of Transfusion Medicine, Juntendo University School of Medicine, Tokyo, Japan. oshaka@med.juntendo.ac.jp
authors.forename: Akimichi
authors.initials: A
authors.lastname: Ohsaka
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hoshino
authors.forename: Masaru
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kudo
authors.forename: Ryuji
authors.initials: R
authors.lastname: Kawaguchi
mesh_terms: D000368:Aged; D001752:Blast Crisis; D020732:Cytogenetic Analysis; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016044:Fusion Proteins, bcr-abl