Phosphatidylinositol(PI)-3 Kinase Contributes to the Activation of Stat3 in M1 Leukemia Cells Induced by IL-6.
Wortmannin, a specific inhibitor of PI-3 kinase, antagonized the inhibitory effect of IL-6 in M1 acute myeloid leukemia cells. Electrophoretic mobility shift assays revealed that wortmannin selectively reduced the IL-6-induced activation of Stat3, while Stat3 plays a central role in IL-6-induced growth-arrest and terminal differentiation of M1 cells. These results indicated that PI-3 kinase was involved in the signal transduction of IL-6 and contributed to the activation of Stat3.
Zhang
Ji-Yan
JY
Department of Immunology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China. jiyanzhang@hotmail.com
Shen
Bei-Fen
BF