title: Seroprevalence of Borrelia burgdorferi in Romania.
abstract: In order to assess the seroprevalence of Borrelia burgdorferi in Romania and to define associated risk factors, a cross-sectional, observational study was performed in 13 districts during 1999. Sera from healthy blood donors (1,598) and from forestry workers (1,048) were tested. A two-step testing strategy was used in which sera were tested for anti-B. burgdorferi antibodies by a commercially available passive hemagglutination assays (PHA). All PHA positive sera were then evaluated by Western blot IgG. Demographic data regarding age, sex, profession, work place/residence, duration of employment (forestry workers), animals in the environment, and tick bites history were collected using a questionnaire. Data obtained from serological study were matched with that obtained from the questionnaire. The seroprevalence of B. burgdorferi in blood donors was of 4.3% (range 1.4-8.7%) and 9.3% (range 2.8-31.7%) in forestry workers. Seroprevalence was higher in forestry workers with a tick bite history (10.7 vs. 4.3%, p < 0.05). The highest seroprevalence in blood donors (8.7%) was noted in Maramures, a northern district of the country, whereas in forestry workers the highest seroprevalence (31.7%) was observed in a western district (Arad), where a previous study in entomology has demonstrated the highest density of Ixodes ricinus ticks in Romania.
authors.affiliation: Infectious Diseases Department, Institute of Infectious Diseases Prof Dr Matei Bals, University of Medicine and Pharmacy Carol Davila Bucharest, Romania. ahristea@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: Hristea
authors.forename: S
authors.initials: S
authors.lastname: Hristescu
authors.forename: C
authors.initials: C
authors.lastname: Ciufecu
authors.forename: A
authors.initials: A
authors.lastname: Vasile
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D025065:Borrelia burgdorferi; D003430:Cross-Sectional Studies; D005260:Female; D016468:Forestry; D006386:Hemagglutination Tests; D006801:Humans; D008193:Lyme Disease / Q000453:epidemiology*; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D012383:Romania / Q000453:epidemiology; D016036:Seroepidemiologic Studies
chemical_list: D000907:Antibodies, Bacterial