Microarray analysis of corneal fibroblast gene expression after interleukin-1 treatment.
OBJECTIVE
To identify changes in gene expression in human corneal fibroblasts after exposure to interleukin-1alpha.


METHODS
RNA was isolated from cultured human corneal fibroblasts after treatment with interleukin-1alpha and subjected to DNA microarray analysis. Changes in gene expression were determined by comparison with untreated cells in three independent experiments after a Bayesian statistical analysis of variance.


RESULTS
Changes in gene expression were reproducibly observed in 165 genes representing previously identified and novel chemokines, matrix molecules, membrane receptors, angiogenic mediators, and transcription factors that correlated with pathophysiological responses to inflammation. Dramatic increases in gene expression were observed with exodus-1 (CCL20), MMP-12, and RhoA.


CONCLUSIONS
DNA microarray analysis of the corneal fibroblast response to interleukin-1alpha provides important insight into modeling changes in gene expression and suggests novel therapeutic targets for the control of corneal inflammation.
Mahajan
Vinit B
VB
Department of Microbiology and Molecular Genetics, University of California-Irvine, Irvine, CA 92697, USA.
Wei
Cui
C
McDonnell
Peter J
PJ
D002478:Cells, Cultured; D019295:Computational Biology / Q000379:methods; D003315:Cornea / Q000187:drug effects* / Q000378:metabolism; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000302:isolation & purification
D005136:Eye Proteins; D007375:Interleukin-1; D012313:RNA