Kinetic model of DNA replication in eukaryotic organisms.
We formulate a kinetic model of DNA replication that quantitatively describes recent results on DNA replication in the in vitro system of Xenopus laevis prior to the mid-blastula transition. The model describes well a large amount of different data within a simple theoretical framework. This allows one, for the first time, to determine the parameters governing the DNA replication program in a eukaryote on a genome-wide basis. In particular, we have determined the frequency of origin activation in time and space during the cell cycle. Although we focus on a specific stage of development, this model can easily be adapted to describe replication in many other organisms, including budding yeast.
Herrick
John
J
Unité de Stabilité des Génomes, Département Structure et Dynamique des Génomes, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France.
Jun
Suckjoon
S
Bechhoefer
John
J
Bensimon
Aaron
A
D000818:Animals; D003198:Computer Simulation; D004261:DNA Replication; D005057:Eukaryotic Cells; D007700:Kinetics; D008957:Models, Genetic; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics