title: Estrogen deficiency results in enhanced expression of Smoothened of the Hedgehog signaling in the thymus and affects thymocyte development.
abstract: Aromatase is an essential enzyme for estrogen synthesis. We investigated the role of estrogen in thymocyte development using aromatase-deficient (ArKO) mice. Like its role as a regulator of bone metabolism through regulating osteoprotegerin (OPG) production, estrogen is involved in the processes of thymocyte development although aromatase mRNA was not detectable in the thymus. Thymic regression and reduced cellularity were evident in ArKO mice. The major difficulties in thymocyte development of ArKO mice were observed during the CD44+ CD25- stage at the cortico-medullary junction and during the CD44- CD25- stage at the subcapsular region where the estrogen receptor was expressed in the stromal cells. The proportion of thymocytes during the CD44+ CD25- stage was reduced. The progression of CD44- CD25+ cells to the CD44- CD25- stage was accelerated in ArKO mice possibly due to insufficient osteoprotegerin production in estrogen-deficiency. However, the expression of Smoothened of the Hedgehog signaling was enhanced in CD4- CD8- double negative cells. This enhancement may result in impaired progression of CD44- CD25- cells to the CD4+ CD8+ double positive stage and impaired proliferation of CD4+ CD8+ double positive cells since Smoothened (Smo) is known to arrest cells as non-proliferating cells. This could be the reason why the proportion of CD3+ TCRbeta(high) cells during the late phase of thymocyte maturation was reduced in ArKO mice. From these observations, we propose that estrogen supports thymocyte development and maturation at many stages through many regulatory pathways including the sonic hedgehog- and the osteoprotegerin ligand (OPGL)-mediated signaling.
authors.affiliation: Department of Medicine, Kochi Medical School, Japan.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Li
authors.forename: Katsumi
authors.initials: K
authors.lastname: Toda
authors.forename: Toshiji
authors.initials: T
authors.lastname: Saibara
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Masafumi
authors.initials: M
authors.lastname: Ono
authors.forename: Shinji
authors.initials: S
authors.lastname: Iwasaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Maeda
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Okada
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Enzan
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Shizuta
authors.forename: Saburo
authors.initials: S
authors.lastname: Onishi
mesh_terms: D000818:Animals; D001141:Aromatase / Q000172:deficiency* / Q000235:genetics; D004967:Estrogens / Q000172:deficiency* / Q000235:genetics; D005260:Female; D053823:Hedgehog Proteins; D018960:Hyaluronan Receptors / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D043562:Receptors, G-Protein-Coupled; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D000072086:Smoothened Receptor; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism*; D015534:Trans-Activators / Q000096:biosynthesis / Q000502:physiology*
chemical_list: D004967:Estrogens; D053823:Hedgehog Proteins; D018960:Hyaluronan Receptors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D015375:Receptors, Interleukin-2; C465570:Shh protein, mouse; C491022:Smo protein, mouse; D000072086:Smoothened Receptor; D015534:Trans-Activators; D001141:Aromatase