title: Antigen presentation by Peyer's patch cells can induce both Th1- and Th2-type responses depending on antigen dosage, but a different cytokine response pattern from that of spleen cells.
abstract: The Th1 and Th2 preference induced by cells from the Peyer's patch (PP) and spleen (SPL) with various doses of an antigen was examined. The same splenic T cell receptor-transgenic CD4+ T cells were first incubated with PP or SPL cells in the presence of various doses of an antigen, and the cytokine response was observed after secondary stimulation. A Th2-type pattern was only obtained for primary stimulation at 10 microM of the antigen with PP cells, whereas a Th1 pattern was induced at both higher and lower concentrations. SPL cells in the presence of 0.1 to 1 microM of the antigen induced the secretion of Th2-type cytokines. Ten and 100 microM of the antigen plus SPL cells did not induce the release of a large quantity of cytokines. PP cells induced a different cytokine pattern at the antigen concentration that induced a similar level of T cell proliferation with SPL cells. Our findings suggest that the antigen-dose dependent development of Th1/Th2 cells is differentially modulated by the antigen-presentation function of cells in PP and SPL.
authors.affiliation: Department of Applied Biological Chemistry, University of Tokyo, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hachimura
authors.forename: Mina
authors.initials: M
authors.lastname: Ishimori
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Kinugasa
authors.forename: Wataru
authors.initials: W
authors.lastname: Ise
authors.forename: Mamoru
authors.initials: M
authors.lastname: Totsuka
authors.forename: Akio
authors.initials: A
authors.lastname: Ametani
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaminogawa
mesh_terms: D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D016207:Cytokines / Q000378:metabolism*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D010581:Peyer's Patches / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D000941:Antigens; D016207:Cytokines