title: ELVIS: a new 24-hour culture test for detecting herpes simplex virus from ocular samples.
abstract: OBJECTIVE
  To compare ELVIS (Enzyme Linked Virus Inducible System) (BioWhittaker, Walkersville, Md), a new, simple, 24-hour cell culture test for detecting herpes simplex virus (HSV), with standard cell culture and Herpchek (NEN, Boston, Mass) for detecting HSV in ocular specimens.
  
  
  METHODS
  Retrospectively, 36 true-positive frozen-stock ocular samples that were cell-culture positive for HSV, and 25 true-negative samples (varicella-zoster virus, adenovirus, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus viridans) were tested with ELVIS. Herpchek was processed at the time of initial clinical laboratory testing. Prospectively, 422 patients were tested for HSV with standard cell culture, ELVIS, and Herpchek. The sensitivity, specificity, positive and negative predictive values, and efficacy of ELVIS based on positive and negative cell cultures were determined.
  
  
  RESULTS
  Retrospectively, ELVIS was 86.1% sensitive (31/36), 100% specific (25/25), and 91.8% efficient (56/61). The positive predictive value was 100% (31/31), and the negative predictive value was 83.3% (25/30). The sensitivity of ELVIS was equivalent to Herpchek (80.5%, 29/36) (P =.53). Prospectively, the sensitivity of ELVIS (84.8%, 28/33) was equivalent to that of Herpchek (84.8%, 28/33).
  
  
  CONCLUSIONS
  ELVIS is an easy HSV diagnostic test that can provide faster positive culture results than standard cell culture, and it is equally sensitive but less time-consuming than Herpchek.
authors.affiliation: Charles T. Campbell Ophthalmic Microbiology Laboratory,University of Pittsburgh Medical Center, Pittsburgh, PA, USA. kowalskirp@msx.upmc.edu
authors.forename: Regis P
authors.initials: RP
authors.lastname: Kowalski
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Karenchak
authors.forename: Chirag
authors.initials: C
authors.lastname: Shah
authors.forename: Jerold S
authors.initials: JS
authors.lastname: Gordon
mesh_terms: D003228:Conjunctiva / Q000821:virology; D003315:Cornea / Q000821:virology; D005189:False Positive Reactions; D018259:Herpesvirus 1, Human / Q000302:isolation & purification* / Q000502:physiology; D018258:Herpesvirus 2, Human / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D016849:Keratitis, Herpetic / Q000821:virology*; D011237:Predictive Value of Tests; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D011933:Reagent Kits, Diagnostic