Poly(L-lysine)-g-poly(D,L-lactic-co-glycolic acid) micelles for low cytotoxic biodegradable gene delivery carriers.
Poly(lactic-co-glycolic acid) (PLGA)-grafted poly(L-lysine) (PLL) (PLL-g-PLGA) was synthesized to demonstrate its micelle-forming property in an aqueous solution. The micelles were used as a gene delivery carrier. The hydrodynamic diameter of PLL-g-PLGA micelles in an aqueous solution was ca. 149 nm with a narrow size distribution. Critical micelle concentration (cmc) was 9.6 mg/l. The PLL-g-PLGA micelles could be used to produce compact nanoparticulate complexes with plasmid DNA, which could efficiently protect the complexed DNA from enzymatic degradation by DNase I. The micelle/DNA complexes had highly compacted structure sized between 200-300 nm with a positive surface charge value. The PLL-g-PLGA micelles exhibited much higher transfection efficiency with lower cytotoxicity than PLL. Here, we demonstrated that biodegradable and cationic PLL-g-PLGA micelles could be used as an effective DNA condensation carrier for gene delivery system.
Jeong
Ji Hoon
JH
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea.
Park
Tae Gwan
TG
D016475:3T3 Cells; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D018014:Gene Transfer Techniques; D008239:Lysine; D051379:Mice; D008823:Micelles; D010957:Plasmids / Q000737:chemistry; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection
D004337:Drug Carriers; D008823:Micelles; D011091:Polyesters; D011506:Proteins; C466993:poly(lactic-co-glycolic acid)-grafted-poly(L-lysine); D004247:DNA; D008239:Lysine