Software and methods for oligonucleotide and cDNA array data analysis.
Two HTML-based programs were developed to analyze and filter gene-expression data: 'Bullfrog' for Affymetrix oligonucleotide arrays and 'Spot' for custom cDNA arrays. The programs provide intuitive data-filtering tools through an easy-to-use interface. A background subtraction and normalization program for cDNA arrays was also built that provides an informative summary report with data-quality assessments. These programs are freeware to aid in the analysis of gene-expression results and facilitate the search for genes responsible for interesting biological processes and phenotypes.
Zapala
Matthew A
MA
The Salk Institute for Biological Studies, Laboratory of Genetics, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA. dlockhart@ambitbio.com
Lockhart
Daniel J
DJ
Pankratz
Daniel G
DG
Garcia
Anthony J
AJ
Barlow
Carrolee
C
Lockhart
David J
DJ
D019295:Computational Biology / Q000295:instrumentation / Q000379:methods; D018076:DNA, Complementary / Q000032:analysis* / Q000235:genetics; D003628:Database Management Systems / Q000639:trends; D030541:Databases, Genetic / Q000639:trends; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D009841:Oligonucleotides / Q000235:genetics*; D012984:Software / Q000639:trends
D018076:DNA, Complementary; D009841:Oligonucleotides