The epithelial-specific adaptor AP1B mediates post-endocytic recycling to the basolateral membrane.
To perform vectorial secretory and transport functions that are critical for the survival of the organism, epithelial cells sort plasma membrane proteins into polarized apical and basolateral domains. Sorting occurs post-synthetically, in the trans Golgi network (TGN) or after internalization from the cell surface in recycling endosomes, and is mediated by apical and basolateral sorting signals embedded in the protein structure. Basolateral sorting signals include tyrosine motifs in the cytoplasmic domain that are structurally similar to signals involved in receptor internalization by clathrin-coated pits. Recently, an epithelial-specific adaptor protein complex, AP1B, was identified. AP-1B recognizes a subset of basolateral tyrosine motifs through its mu 1B subunit. Here, we characterized the post-synthetic and post-endocytic sorting of the fast recycling low density lipoprotein receptor (LDLR) and transferrin receptor (TfR) in LLC-PK1 cells, which lack mu 1B and mis-sort both receptors to the apical surface. Targeting and recycling assays in LLC-PK1 cells, before and after transfection with mu 1B, and in MDCK cells, which express mu 1B constitutively, suggest that AP1B sorts basolateral proteins post-endocytically.
Gan
Yunbo
Y
Dyson Vision Research Institute, Weill Medical College of Cornell University, 1300 York Ave., New York, NY 10021, USA.
McGraw
Timothy E
TE
Rodriguez-Boulan
Enrique
E
D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002966:Clathrin / Q000378:metabolism; D004285:Dogs; D004705:Endocytosis; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D018374:LLC-PK1 Cells; D008565:Membrane Proteins / Q000378:metabolism*; D011973:Receptors, LDL / Q000378:metabolism; D011990:Receptors, Transferrin / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013552:Swine
D033942:Adaptor Proteins, Vesicular Transport; D002352:Carrier Proteins; D002966:Clathrin; D008565:Membrane Proteins; D011973:Receptors, LDL; D011990:Receptors, Transferrin; D011994:Recombinant Proteins