Monitoring the identity of bacteria using their intrinsic fluorescence.
Three different fluorescence spectra were recorded following excitation at 250 nm (aromatic amino acids+nucleic acids), 270 nm (tryptophan residues) and 316 nm (NADH) for 25 strains of bacteria in dilute suspensions. Evaluation of the spectra using principal component analysis and hierarchical clustering showed a good reproducibility from culture to culture and a good discrimination of the bacteria. Applying the method of Mahalanobis distances to the spectra of lactobacilli species recorded following excitation at 250 nm, a good classification was observed for 100% and 81% of calibration and validation groups, respectively. The developed method allows the discrimination and identification of the investigated bacteria at the genus, species and strain levels.
Leblanc
Ludovic
L
UPRES Typicité des Produits Alimentaires, ENITA Clermont Ferrand, site de Marmilhat, 63370, Lempdes, France.
Dufour
Eric
E
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005453:Fluorescence; D013294:Lactococcus lactis / Q000737:chemistry / Q000302:isolation & purification; D008087:Listeria / Q000737:chemistry / Q000302:isolation & purification; D010376:Pediococcus / Q000737:chemistry / Q000302:isolation & purification; D010802:Phylogeny; D011551:Pseudomonas fluorescens / Q000737:chemistry / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence / Q000379:methods*