Laccase-catalyzed oxidation of Mn(2+) in the presence of natural Mn(3+) chelators as a novel source of extracellular H(2)O(2) production and its impact on manganese peroxidase.
A purified and electrophoretically homogeneous blue laccase from the litter-decaying basidiomycete Stropharia rugosoannulata with a molecular mass of approximately 66 kDa oxidized Mn(2+) to Mn(3+), as assessed in the presence of the Mn chelators oxalate, malonate, and pyrophosphate. At rate-saturating concentrations (100 mM) of these chelators and at pH 5.0, Mn(3+) complexes were produced at 0.15, 0.05, and 0.10 micromol/min/mg of protein, respectively. Concomitantly, application of oxalate and malonate, but not pyrophosphate, led to H(2)O(2) formation and tetranitromethane (TNM) reduction indicative for the presence of superoxide anion radical. Employing oxalate, H(2)O(2) production, and TNM reduction significantly exceeded those found for malonate. Evidence is provided that, in the presence of oxalate or malonate, laccase reactions involve enzyme-catalyzed Mn(2+) oxidation and abiotic decomposition of these organic chelators by the resulting Mn(3+), which leads to formation of superoxide and its subsequent reduction to H(2)O(2). A partially purified manganese peroxidase (MnP) from the same organism did not produce Mn(3+) complexes in assays containing 1 mM Mn(2+) and 100 mM oxalate or malonate, but omitting an additional H(2)O(2) source. However, addition of laccase initiated MnP reactions. The results are in support of a physiological role of laccase-catalyzed Mn(2+) oxidation in providing H(2)O(2) for extracellular oxidation reactions and demonstrate a novel type of laccase-MnP cooperation relevant to biodegradation of lignin and xenobiotics.
Schlosser
Dietmar
D
Microbiology of Subterrestrial Aquatic Systems Group, UFZ Centre for Environmental Research Leipzig-Halle, Theodor-Lieser-Strasse 4, D-06120 Halle, Germany. schloss@hdg.ufz.de
Höfer
Christine
C
D000363:Agaricales / Q000201:enzymology*; D002384:Catalysis; D002614:Chelating Agents / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D042845:Laccase; D008345:Manganese / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*
D002614:Chelating Agents; D008345:Manganese; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase; D010100:Oxygen