Expression of beta-1,4-galactosyltransferase II and V in rat injured sciatic nerves.
Beta-1,4-Galactosyltransferases II and V (beta-1,4-GalT II and V) are involved in the biosynthesis of N-linked oligosaccharides as beta-1,4-GalT I which plays important roles in promoting neuron outgrowth. In the present paper, it was illustrated that beta-1,4-GalT II and V were localized mainly in Schwann cells of lesion sciatic nerves by in situ hybridization. Northern blot showed that the expression of beta-1,4-GalT II increased gradually at both stumps of injured nerves, while that of beta-1,4-GalT V decreased at proximal stumps but increased and reached its peak on the third day post-operation at distal stumps, before it declined. The different expression of beta-1,4-GalT II and V in Schwann cells suggested that they would affect the different galactosylation of glycoproteins in injured nerves regeneration.
Shen
Aiguo
A
Box 103, Gene Research Center, Medical Center of Fudan University (Former Shanghai Medical University), 200032, Shanghai, People's Republic of China.
Wang
Hanzhou
H
Zhang
Yong
Y
Yan
Jun
J
Zhu
Dan
D
Gu
Jianxin
J
D000818:Animals; D015152:Blotting, Northern; D005260:Female; D005700:Galactosyltransferases / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D017403:In Situ Hybridization; D009416:Nerve Regeneration / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000201:enzymology; D012584:Sciatic Nerve / Q000166:cytology / Q000201:enzymology* / Q000293:injuries*
D012333:RNA, Messenger; D005700:Galactosyltransferases; C419407:beta-1,4-galactosyltransferase II; C419408:beta-1,4-galactosyltransferase V