KAP-2, a protein that binds to the H-box in a bean chalcone synthase promoter, is a novel plant transcription factor with sequence identity to the large subunit of human Ku autoantigen.
The KAP-2 protein that binds to the H-box (CCTACC) element in the bean CHS15 chalcone synthase promoter was purified, and internal peptide sequence used to design primers leading to the cloning of KAP-2 from bean (Phaseolus vulgaris) and barrel medic (Medicago truncatula). KAP-2 shares sequence similarity to the large subunit of mammalian Ku autoantigen, a protein proposed to be involved in control of DNA recombination and transcription. KAP-2 sequences were present in genomic DNA from a range of legumes, and a related protein is found in Arabidopsis thaliana. Recombinant KAP-2 expressed in insect cells showed the same binding specificity for the CHS15 H-box as the protein purified from bean cell extracts. In vitro transcription assays confirmed that KAP-2 stimulates transcription from a promoter harboring the H-box cis element.
Lindsay
William P
WP
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73401, USA.
McAlister
Fiona M
FM
Zhu
Qun
Q
He
Xian-Zhi
XZ
Dröge-Laser
Wolfgang
W
Hedrick
Susie
S
Doerner
Peter
P
Lamb
Chris
C
Dixon
Richard A
RA
D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D034961:Antigens, Nuclear; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D004265:DNA Helicases; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D007887:Fabaceae / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology; D005786:Gene Expression Regulation; D006801:Humans; D000072200:Ku Autoantigen; D029902:Medicago / Q000235:genetics / Q000382:microbiology; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D027805:Phaseolus / Q000201:enzymology / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D016962:Sinorhizobium meliloti / Q000254:growth & development; D018411:Spodoptera / Q000166:cytology / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
D034961:Antigens, Nuclear; D018076:DNA, Complementary; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009841:Oligonucleotides; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D000217:Acyltransferases; C020141:flavanone synthetase; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen