The membrane-associated F(0)F(1) ATPase is essential for the viability of Streptococcus pneumoniae.
Genetic studies aimed at eliminating expression of the atp operon (F(0)F(1) H(+)-ATPase) of Streptococcus pneumoniae by genetic disruption of atpC, the first gene of the operon, with a chloramphenicol-resistance cassette were performed. Resistant transformants were obtained only when the recipient strain had a duplication of atpC, recombination occurring in such a way that transcription of the operon from its own promoter was allowed. These results imply that the atp operon is essential for the viability of the cells.
Ferrándiz
María José
MJ
Unidad de Genética Bacteriana (Consejo Superior de Investigaciones Científicas), Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain
de la Campa
Adela G
AG
D002462:Cell Membrane / Q000201:enzymology*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D020043:Genes, Essential; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000254:growth & development*; D014169:Transformation, Bacterial
D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase; C103607:mitochondrial ATPase subunit c; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases