A polygalacturonase of animal origin isolated from the root-knot nematode Meloidogyne incognita.
The first animal polygalacturonase (PG, EC 2.1.15) encoding cDNA, Mi-pg-1, was cloned from the plant parasitic nematode Meloidogyne incognita. The enzymatic activity of MI-PG-1 was confirmed after heterologous expression in Escherichia coli. The presence of a predicted signal peptide on the MI-PG-1 sequence together with the specific localization of the transcripts of the Mi-pg-1 gene in the oesophageal glands of infective juveniles imply that MI-PG-1 could be secreted into plant tissues. The potential role of MI-PG-1 in parasitism is discussed.
Jaubert
Stéphanie
S
INRA, Unité Interactions Plantes-Microorganismes et Santé Végétale, 123 Boulevard Francis Meilland, 60606 Antibes, France.
Laffaire
Jean-Baptiste
JB
Abad
Pierre
P
Rosso
Marie-Noëlle
MN
D000818:Animals; D001483:Base Sequence; D004926:Escherichia coli; D015870:Gene Expression; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000469:parasitology; D011096:Polygalacturonase / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014431:Tylenchoidea / Q000201:enzymology* / Q000235:genetics
D011096:Polygalacturonase