Cell-cycle dependent dynamic change of 26S proteasome distribution in tobacco BY-2 cells.
The 26S proteasome is known to play pivotal roles in cell-cycle progression in various eukaryotic cells; however, little is known about its role in higher plants. Here we report that the subcellular distribution of the 26S proteasome is dynamically changed in a cell-cycle dependent manner in tobacco BY-2 cells as determined by immunostaining with anti-Rpn10 (a regulatory PA700 subunit) and anti-20S catalytic proteasome antibodies. The 26S proteasome was found to localize not only in nuclear envelopes and mitotic spindles but also in preprophase bands (PPBs) and phragmoplasts appearing in G(2) and M phases, respectively. MG132, a proteasome inhibitor, exclusively caused cell-cycle arrest not only at the metaphase but also the early stage of PPB formation at the G(2) phase and the collapse of the phragmoplast, which seems to be closely related to proteasome distribution in the cells.
Yanagawa
Yuki
Y
Department of Bioproduction Science, Faculty of Horticulture, Chiba University, Matsudo, Matsudo, Chiba, 271-8510 Japan.
Hasezawa
Seiichiro
S
Kumagai
Fumi
F
Oka
Masayoshi
M
Fujimuro
Masahiro
M
Naito
Tae
T
Makino
Taro
T
Yokosawa
Hideyoshi
H
Tanaka
Keiji
K
Komamine
Atsushi
A
Hashimoto
Junji
J
Sato
Takahide
T
Nakagawa
Hiroki
H
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D003546:Cysteine Endopeptidases / Q000378:metabolism; D016195:G2 Phase / Q000502:physiology; D007976:Leupeptins / Q000494:pharmacology; D008677:Metaphase / Q000502:physiology; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis / Q000502:physiology; D009097:Multienzyme Complexes / Q000378:metabolism; D009685:Nuclear Envelope / Q000378:metabolism; D010447:Peptide Hydrolases / Q000187:drug effects / Q000378:metabolism*; D011418:Prophase / Q000502:physiology; D046988:Proteasome Endopeptidase Complex; D011506:Proteins / Q000378:metabolism; D018724:Spinacia oleracea / Q000166:cytology; D008941:Spindle Apparatus / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
D002352:Carrier Proteins; D007976:Leupeptins; D009097:Multienzyme Complexes; C085834:PA700 proteasome activator; D011506:Proteins; D010447:Peptide Hydrolases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease; D000251:Adenosine Triphosphatases; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde