The expression of dentin sialophosphoprotein gene in bone.
Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP) are expressed as a single mRNA transcript coding for a large precursor protein termed dentin sialophosphoprotein (DSPP). DSP, DPP, and DSPP have been considered to be tooth-specific. To test for the expression of the dspp gene in bone, we performed Western immunoblots and reverse-transcription polymerase chain-reaction (RT-PCR). With Western immunoblots, we detected DSP in the Gdm/EDTA extracts of rat long bone, at a level of about 1/400 of that in dentin. Using RT-PCR, we detected DSPP mRNA in mouse calvaria. Similar to Western immunoblots, the results of RT-PCR indicated that the dspp gene is expressed at a lower level in bone than in dentin and odontoblasts. Analysis of the data shows that DSPP is not a tooth-specific protein, and that dramatically different regulatory mechanisms governing DSPP expression are involved in the bone and dentin.
Qin
C
C
Department of Basic Sciences, The University of Texas-Houston Health Science Center Dental Branch, 6516 M.D. Anderson Boulevard, DBB Rm 4.133, Houston, TX 77030, USA. cqin@mail.db.uth.tmc.edu
Brunn
J C
JC
Cadena
E
E
Ridall
A
A
Tsujigiwa
H
H
Nagatsuka
H
H
Nagai
N
N
Butler
W T
WT
D000818:Animals; D015153:Blotting, Western; D001842:Bone and Bones / Q000378:metabolism*; D003804:Dentin / Q000378:metabolism; D016326:Extracellular Matrix Proteins; D015870:Gene Expression; D051379:Mice; D009928:Organ Specificity; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000235:genetics; D000099311:Dentin Sialophosphoprotein
D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein