title: Impaction grafting with morsellised allograft and tricalcium phosphate-hydroxyapatite: incorporation within ovine metaphyseal bone defects.
abstract: An ovine model was used to investigate the in vivo properties of impacted tricalcium phosphate-hydroxyapatite (TCP-HA) aggregates, varying in chemical composition (ratio of TCP to HA) and particle size distribution (8 versus 3 particle size ranges). All pellets were impacted to a standard compactive effort. Eight sheep underwent implantation of pellets in 4 metaphyseal defects in both rear limbs. Treatment groups consisted of: (1) allograft (clinical control). (2) 50/50 allograft/80% HA/20% TCP in 8 particle size ranges, (3) 50/50 allograft/80% TCP/20% HA in 8 sizes and (4) 50/50 allograft/80% HA/20% TCP in only 3 sizes of particles. Healing of defects was evaluated at 14 weeks with computed tomography, histology and histomorphometry. The computer tomography (CT) density measured in all defects containing synthetic agents was higher than in defects filled with allograft alone (p<0.01). Defects containing 8 sizes of 80% HA/ 20% TCP granules (group 2) achieved lower histological scores and contained less bone than the clinical control (p<0.05), whereas groups 3 and 4 did not differ from the control. Although all synthetic agents were osteoconductive, our results suggest that increasing the ratio of TCP over HA and limiting the number of particle size ranges to 3 instead of 8 improve the performance of impacted aggregates as graft expanders. Evaluation under loading conditions of morsellised allograft expanded with 80% TCP/20% HA (BoneSave) in 3 particle size ranges is warranted.
authors.affiliation: Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian, UK.
authors.forename: J N J
authors.initials: JN
authors.lastname: Pratt
authors.forename: D J
authors.initials: DJ
authors.lastname: Griffon
authors.forename: D G
authors.initials: DG
authors.lastname: Dunlop
authors.forename: N
authors.initials: N
authors.lastname: Smith
authors.forename: C R
authors.initials: CR
authors.lastname: Howie
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000601:surgery*; D018786:Bone Substitutes / Q000627:therapeutic use; D002130:Calcium Phosphates / Q000627:therapeutic use*; D006614:Hindlimb; D006882:Hydroxyapatites / Q000627:therapeutic use*; D012756:Sheep; D014057:Tomography, X-Ray Computed; D014184:Transplantation, Homologous / Q000379:methods*
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D006882:Hydroxyapatites; C485817:beta-tricalcium phosphate; C018392:tricalcium phosphate