Urocortin promotes hemodynamic and bioenergetic recovery and improves cell survival in the isolated rat heart exposed to ischemia/reperfusion.
OBJECTIVE
This study evaluates the hemodynamic, bioenergetic and cytoprotective effects of urocortin (Ucn) in the isolated rat heart exposed to ischemia (I)/reperfusion (R).


BACKGROUND
We have previously demonstrated that administration of exogenous Ucn reduces infarct size in ischemic-reperfused rat hearts.


METHODS
Urocortin 10(-8)M was added to the perfusate before I, before I and during R, and during R alone in the isolated pulsed rat heart exposed to 35 min I followed by 60 min R.


RESULTS
Partial to complete recovery of diastolic pressure and developed pressure was seen irrespective of when Ucn was perfused. In particular, beneficial effects are observed when Ucn is only given during R. Urocortin given only before I, and before I and over R, although not during R alone, also produces significant recovery of high-energy phosphate pools. In each group, improvement in ventricular function is associated with reduction both in myocardial damage, assessed by creatine phosphokinase release, and in endothelial cell and cardiomyocyte apoptosis, assessed by caspase 3 activity and fluorescent-based terminal deoxynucleotidyl transferase mediated nick end labelling enhanced with counterstains. These improvements in ventricular performance, bioenergetics and cell survival are not secondary to any inotropic effects of Ucn.


CONCLUSIONS
This is the first report to show enhanced cardiac function induced by Ucn during I/R. Because the cytoprotective and functional benefits are still produced when Ucn is given only at R, these data suggest that Ucn may be useful clinically in the management of myocardial infarction.
Scarabelli
Tiziano M
TM
Medical Molecular Biology Unit, Institute of Child Health and Great Ormond Street Hospital, University College London, London, United Kingdom. t.scarabelli@ich.ucl.ac.uk
Pasini
Evasio
E
Stephanou
Anastasis
A
Comini
Laura
L
Curello
Salvatore
S
Raddino
Riccardo
R
Ferrari
Roberto
R
Knight
Richard
R
Latchman
David S
DS
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D003402:Creatine Kinase / Q000378:metabolism; D004792:Enzyme Precursors / Q000494:pharmacology; D006321:Heart / Q000187:drug effects; D006439:Hemodynamics / Q000187:drug effects; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology*; D009206:Myocardium / Q000201:enzymology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010477:Perfusion; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D054832:Urocortins
D004792:Enzyme Precursors; D018696:Neuroprotective Agents; D054832:Urocortins; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D003346:Corticotropin-Releasing Hormone; D003402:Creatine Kinase; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases