cDNA cloning of the Sry-related gene Sox6 from rat with tissue-specific expression.
A cDNA encoding rat homologue of the previously characterized mouse Sox6 was isolated by a polymerase chain reaction (PCR) cloning strategy. Comparison of this eDNA with homologous mouse, human and rainbow trout cDNA exhibited an overall amino acid sequence identity of 99.6, 89.3 and 76.3% respectively. The leucine-zipper and HMG-box motif were almost completely conserved between these homologues. The expression of Sox6 was determined in rat by Northern hybridization and Real-time quantitative reverse transcription (RT)-PCR. rSox6 (rat Sox6) was specifically expressed in the neonatal brain and adult testis with Northern blotting. Real-time quantitative RT-PCR for the determination of Sox6 mRNA was examined. The rSox6 was expressed in the neonatal brain and adult testis as well as by Northern blotting and also expressed in the adult eyeball and slightly in the ovary.
Narahara
Masanori
M
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan. narahara@pharm.kobegakuin.ac.jp
Yamada
Akihisa
A
Hamada-Kanazawa
Michiko
M
Kawai
Yuichi
Y
Miyake
Masaharu
M
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D004926:Escherichia coli / Q000378:metabolism; D006609:High Mobility Group Proteins / Q000096:biosynthesis* / Q000235:genetics*; D008297:Male; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055755:SOXD Transcription Factors; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution; D014157:Transcription Factors; D014212:Tretinoin / Q000378:metabolism
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D012333:RNA, Messenger; C486270:SOX6 protein, human; D055755:SOXD Transcription Factors; D014157:Transcription Factors; D014212:Tretinoin