Effect of arsenic, cadmium and lead on the induction of apoptosis of normal human mononuclear cells.
The aim of this work was to investigate the effect of cadmium, lead and arsenic on the apoptosis of human immune cells. Peripheral blood mononuclear cells (MNC) were incubated with increasing concentrations of these metals and then cellular apoptosis was determined by flow cytometry and by DNA electrophoresis. We found that arsenic induced a significant level of apoptosis at 15 microM after 48h of incubation. Cadmium had a similar effect, but at higher concentrations (65 microM). In addition, cadmium exerted a cytotoxic effect on MNC that seemed to be independent of the induction of apoptosis. In contrast, concentrations of lead as high as 500 microM were nontoxic and did not induce a significant degree of apoptosis. Additional experiments showed that arsenic at concentrations as low as 1.0 microM had a significant pro-apoptotic effect when cells were cultured in the presence of this pollutant for more than 72. Non-T cells were more susceptible than T lymphocytes to the effect of arsenic and cadmium. Interestingly, MNC from children chronically exposed to arsenic showed a high basal rate of apoptosis and a diminished in vitro sensibility to this metalloid. Our results indicate that both arsenic and cadmium are able to induce apoptosis of lymphoid cells, and suggest that this phenomenon may contribute to their immunotoxic effect in vivo.
de la Fuente
H
H
Department of Immunology, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, México.
Portales-Pérez
D
D
Baranda
L
L
Díaz-Barriga
F
F
Saavedra-Alanís
V
V
Layseca
E
E
González-Amaro
R
R
D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D001151:Arsenic / Q000009:adverse effects* / Q000652:urine; D018053:Arsenites / Q000494:pharmacology / Q000633:toxicity; D002104:Cadmium / Q000009:adverse effects*; D019256:Cadmium Chloride / Q000494:pharmacology / Q000633:toxicity; D002478:Cells, Cultured / Q000187:drug effects; D002648:Child; D003404:Creatinine / Q000652:urine; D004305:Dose-Response Relationship, Drug; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000009:adverse effects*; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000139:chemically induced; D007854:Lead / Q000009:adverse effects*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects*; D008800:Mexico; D008906:Mining; D009942:Organometallic Compounds / Q000494:pharmacology / Q000633:toxicity; D012397:Rosette Formation; D017670:Sodium Compounds / Q000494:pharmacology / Q000633:toxicity; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects
D018053:Arsenites; D004785:Environmental Pollutants; D009942:Organometallic Compounds; D017670:Sodium Compounds; D002104:Cadmium; D007854:Lead; C017947:sodium arsenite; D003404:Creatinine; D019256:Cadmium Chloride; D001151:Arsenic; C008261:lead acetate