title: Induction of alternatively spliced spermidine/spermine N1-acetyltransferase mRNA in the human kidney cells infected by venezuelan equine encephalitis and tick-borne encephalitis viruses.
abstract: 293 and RH cells derived from human embryo kidney were infected by Venezuelan equine encephalitis and tick-borne encephalitis viruses and cDNA libraries representing cellular mRNAs induced or suppressed due to the infection were prepared using suppressive subtractive hybridization. Among the up-regulated clones the RT-PCR and Northern analyses revealed an unusual transcript of the spermidine/spermine N1-acetyltransferase (SSAT) gene that was shown to be an alternatively spliced form containing an additional 110-bp exon. The alternatively spliced transcript is polyadenylated and can be expected to yield only a truncated 71 amino acid polypeptide. This first evidence of the host gene alternatively spliced mRNA induction by RNA viruses raises the questions of its biological role, regulation mechanisms of alternative splicing, and significance for the virus life cycle.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. natalia@humgen.siobc.ras.ru
authors.forename: Natalia N
authors.initials: NN
authors.lastname: Nikiforova
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Velikodvorskaja
authors.forename: Alla V
authors.initials: AV
authors.lastname: Kachko
authors.forename: Lev G
authors.initials: LG
authors.lastname: Nikolaev
authors.forename: Galina S
authors.initials: GS
authors.lastname: Monastyrskaya
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Lukyanov
authors.forename: Svetlana N
authors.initials: SN
authors.lastname: Konovalova
authors.forename: Elena V
authors.initials: EV
authors.lastname: Protopopova
authors.forename: Victor A
authors.initials: VA
authors.lastname: Svyatchenko
authors.forename: Nikolai N
authors.initials: NN
authors.lastname: Kiselev
authors.forename: Valery B
authors.initials: VB
authors.lastname: Loktev
authors.forename: Eugene D
authors.initials: ED
authors.lastname: Sverdlov
mesh_terms: D000123:Acetyltransferases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004666:Encephalitis Virus, Venezuelan Equine / Q000472:pathogenicity*; D004669:Encephalitis Viruses, Tick-Borne / Q000472:pathogenicity*; D004790:Enzyme Induction; D006801:Humans; D007668:Kidney / Q000166:cytology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D000099228:Diamine N-Acetyltransferase
chemical_list: D000123:Acetyltransferases; D012333:RNA, Messenger; D000099228:Diamine N-Acetyltransferase