[Immunodiagnosis of cysticercosis by simplified western blotting using recombinant fusion protein as antigen].
OBJECTIVE
To provide simple and useful method for the immunodiagnosis of cysticercosis.


METHODS
Fusion protein of beta-galactosidase-Cysticercus cellulosae cDNA was analysed with simplified Western blot.


RESULTS
The positive rate was 93.5% when detecting 124 cases of human cysticercosis sera with four clones (cC1, cC2, cP1 and cH1) and 100% when detecting 38 cases of porcine cysticercosis sera with three clones (cC1, cC2 and cP1), being higher than those using monoclone fusion protein alone. Moreover, the fusion proteins did not react with other parasitosis sera.


CONCLUSIONS
Simplified Western blot analysis using recombinant fusion protein as antigen was highly sensitive and specific and easy to be operated.
Peng
Y
Y
Department of Molecular Biology, Hebei Medical University, Shijiazhuang 050017.
Chen
R
R
Sun
S
S
Dai
J
J
Wang
J
J
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D003551:Cysticercosis / Q000175:diagnosis* / Q000662:veterinary; D003552:Cysticercus / Q000235:genetics; D018076:DNA, Complementary / Q000302:isolation & purification; D018509:DNA, Helminth / Q000302:isolation & purification; D006801:Humans; D007159:Immunologic Tests; D011993:Recombinant Fusion Proteins; D012680:Sensitivity and Specificity; D013553:Swine Diseases / Q000175:diagnosis; D001616:beta-Galactosidase
D018076:DNA, Complementary; D018509:DNA, Helminth; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase