title: Systemic immune responses following infection with Jaagsiekte sheep retrovirus and in the terminal stages of ovine pulmonary adenocarcinoma.
abstract: Jaagsiekte sheep retrovirus (JSRV) is the aetiological agent of ovine pulmonary adenocarcinoma (OPA). To monitor changes in cellular immune function during JSRV infection, lymphoproliferation in response to various mitogens was measured in the blood of conventionally housed and specific-pathogen-free lambs experimentally infected with JSRV until the development of OPA and compared with uninfected control lambs. In addition, blood samples collected from adult field cases in the terminal stages of OPA and control adult sheep were compared. No difference in the proliferative response to phytohaemagglutinin and pokeweed mitogen between the animal groups was detected. In contrast, reduced responses to concanavalin A stimulation were demonstrated in the JSRV-inoculated lambs, prior to the onset of clinical disease, and also in the terminally ill adult sheep. Peripheral blood leukocytes were monitored to identify phenotypic frequency alterations. The CD4 lymphocytopaenia and neutrophilia reported previously in adult OPA cases were demonstrated but similar phenotypic changes were not identified during experimental infection.
authors.affiliation: Medical Microbiology, University of Edinburgh, Medical School, Teviot Place, Edinburgh, UK2.|Moredun Research Institute1 and Veterinary Laboratory Agencies3, Pentland Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: Christina
authors.initials: C
authors.lastname: Summers
authors.affiliation: Medical Microbiology, University of Edinburgh, Medical School, Teviot Place, Edinburgh, UK2.
authors.forename: William
authors.initials: W
authors.lastname: Neill
authors.affiliation: Moredun Research Institute1 and Veterinary Laboratory Agencies3, Pentland Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: Patricia
authors.initials: P
authors.lastname: Dewar
authors.affiliation: Moredun Research Institute1 and Veterinary Laboratory Agencies3, Pentland Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Gonzalez
authors.affiliation: Medical Microbiology, University of Edinburgh, Medical School, Teviot Place, Edinburgh, UK2.
authors.forename: Renate
authors.initials: R
authors.lastname: van der Molen
authors.affiliation: Medical Microbiology, University of Edinburgh, Medical School, Teviot Place, Edinburgh, UK2.
authors.forename: Mary
authors.initials: M
authors.lastname: Norval
authors.affiliation: Moredun Research Institute1 and Veterinary Laboratory Agencies3, Pentland Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: James Michael
authors.initials: JM
authors.lastname: Sharp
mesh_terms: D000818:Animals; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D007111:Immunity, Cellular; D022141:Jaagsiekte sheep retrovirus / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008231:Lymphopenia / Q000473:pathology; D009503:Neutropenia / Q000473:pathology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011648:Pulmonary Adenomatosis, Ovine / Q000276:immunology*; D012756:Sheep / Q000276:immunology*
chemical_list: D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens