title: Effect of topically applied charged particles on healing of colonic anastomoses.
abstract: OBJECTIVE
  Various forms of electrical stimulation can improve wound healing in different tissues, but their application to gastrointestinal tract healing has not been investigated. We assumed that positively charged diethylaminoethyl cross-linked dextran bead (diethylaminoethyl Sephadex [DEAE-S]) particles would have a beneficial effect on the healing of colonic anastomoses.
  
  
  METHODS
  Experimental animal study.
  
  
  METHODS
  Animal research laboratory of a university hospital.
  
  
  METHODS
  Forty female Wistar albino rats.
  
  
  METHODS
  Right colonic transection and anastomosis was performed in 5 animal groups. The control group received no treatment; the placebo group, methylcellulose gel; and the DEAE-S group, DEAE-S in methyl cellulose gel applied topically around the anastomoses. The fecal peritonitis (FP) group underwent cecal ligation and perforation simultaneously with the anastomosis to cause FP; the FP + DEAE-S group also received DEAE-S applied around the anastomoses.
  
  
  METHODS
  After the completion of postoperative day 4, all rats were killed. Anastomotic bursting pressures and hydroxyproline concentrations in perianastomotic tissue were measured and compared.
  
  
  RESULTS
  Mean bursting pressures were 115.1 mm Hg in the control group, 113.6 mm Hg in the placebo group, 159.4 mm Hg in the DEAE-S group, 62.8 mm Hg in the FP group, and 121.1 mm Hg in the FP + DEAE-S group (P =.001, 1-way analysis of variance [ANOVA]). The differences between the control vs DEAE-S groups, placebo vs DEAE-S groups, and FP vs FP + DEAE-S groups were significant (P<.05, t test). Mean hydroxyproline concentrations were 5.2 microg/mg in the control group, 4.9 microg/mg in the placebo group, 5.6 microg/mg in the DEAE-S group, 4.5 microg/mg in the FP group, and 5.4 microg/mg in the FP + DEAE-S group (P =.09, 1-way ANOVA). The difference between the FP and FP + DEAE-S groups was significant (P =.04, t test).
  
  
  CONCLUSIONS
  A positively charged particle, DEAE-S, improves healing of colonic anastomoses in healthy rats and in rats with FP. This inexpensive, nontoxic material is easily applied and deserves further evaluation in gastrointestinal tract healing.
authors.affiliation: Fourth Department of Surgery, Ankara Numune Hospital, Uzmanlar Tip Grubu, 3 Cadde, 41 Sokak, No. 3/1, Bahçelievler, 06500 Ankara, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Guler
authors.forename: Murat
authors.initials: M
authors.lastname: Kologlu
authors.forename: Nuri Aydin
authors.initials: NA
authors.lastname: Kama
authors.forename: Nurten
authors.initials: N
authors.lastname: Renda
authors.forename: Ugur
authors.initials: U
authors.lastname: Gozalan
authors.forename: Yunus Nadi
authors.initials: YN
authors.lastname: Yuksek
authors.forename: Gul
authors.initials: G
authors.lastname: Daglar
mesh_terms: D000287:Administration, Topical; D000714:Anastomosis, Surgical; D000818:Animals; D002412:Cations / Q000627:therapeutic use; D003106:Colon / Q000601:surgery*; D003637:DEAE-Dextran / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D006909:Hydroxyproline / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D002412:Cations; D003637:DEAE-Dextran; D006909:Hydroxyproline