Investigation on lipid asymmetry using lipid probes: Comparison between spin-labeled lipids and fluorescent lipids.
Synthetic lipids with a nitroxide or a fluorescent probe have been extensively used during the last 30 years to determine the transmembrane diffusion of phospholipids in artificial or biological membranes. However, the relevance of data obtained with these modified lipids has sometimes been questioned. Beside possible artefacts introduced by the reporter probe, synthetic lipids used in cells often contain a short fatty acid chain in the sn-2 position, which gives them higher water solubility than naturally occurring lipids. In the present review, we have attempted to give a critical appraisal. Main strategies are recalled and important discoveries obtained with lipid probes on transmembrane lipid traffic in eukaryotic cells are briefly summarized. Examples of artefacts caused by lipid probes are given. Comparisons between data obtained by different techniques such as ESR and fluorescence allow us to emphasize the complementary character of the two approaches and more generally show the necessity to use several probes before drawing conclusions concerning endogenous lipids. In spite of these pitfalls, overall, lipid probes have provided a wealth of useful information that, to date, cannot be obtained with unlabeled lipids.
Devaux
Philippe F
PF
Institut de Biologie Physico-Chimique, UMR CNRS 7099, 13 rue Pierre et Marie Curie, F75005 Paris, France. philippe.devaux@ibpc.fr
Fellmann
Pierre
P
Hervé
Paulette
P
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry*; D004058:Diffusion; D005456:Fluorescent Dyes; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D013113:Spin Labels
D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008055:Lipids; D013113:Spin Labels