title: A novel biodegradable implant for neuronal rescue and regeneration after spinal cord injury.
abstract: After spinal cord injury, the severed neuronal pathways fail to regenerate spontaneously. This study describes a biodegradable implant using poly-beta-hydroxybutyrate (PHB) fibers as carrier scaffold for matrix components and cell lines supporting neuronal survival and regeneration after spinal cord injury. After cervical spinal cord injury in adult rats, a graft consisting of PHB fibers coated with alginate hydrogel + fibronectin was implanted in the lesion cavity. In control groups, PHB was omitted and only alginate hydrogel or fibronectin, or their combination, were used for grafting. In addition, comparisons were made with animals treated intrathecally after spinal cord injury with the neurotrophic factors BDNF or NT-3. The neurons of the rubrospinal tract served as experimental model. In untreated animals, 45% of the injured rubrospinal neurons were lost at 8 weeks postoperatively. Implantation of the PHB graft reduced this cell loss by 50%, a rescuing effect similar to that obtained after treatment with BDNF or NT-3. In the absence of PHB support, implants of only alginate hydrogel or fibronectin, or their combination, had no effect on neuronal survival. After addition of neonatal Schwann cells to the PHB graft, regenerating axons were seen to enter the graft from both ends and to extend along its entire length. These results show that implants using PHB as carrier scaffold and containing alginate hydrogel, fibronectin and Schwann cells can support neuronal survival and regeneration after spinal cord injury
authors.affiliation: Department of Integrative Medical Biology, Umeå University, Sweden.
authors.forename: Lev N
authors.initials: LN
authors.lastname: Novikov
authors.forename: Liudmila N
authors.initials: LN
authors.lastname: Novikova
authors.forename: Afshin
authors.initials: A
authors.lastname: Mosahebi
authors.forename: Mikael
authors.initials: M
authors.lastname: Wiberg
authors.forename: Giorgio
authors.initials: G
authors.lastname: Terenghi
authors.forename: Jan-Olof
authors.initials: JO
authors.lastname: Kellerth
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000493:pharmacokinetics / Q000627:therapeutic use*; D001673:Biodegradation, Environmental; D005260:Female; D006885:Hydroxybutyrates / Q000493:pharmacokinetics / Q000627:therapeutic use*; D007150:Immunohistochemistry; D009410:Nerve Degeneration / Q000503:physiopathology; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D011091:Polyesters / Q000493:pharmacokinetics / Q000627:therapeutic use*; D000091402:Prohibitins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000503:physiopathology* / Q000628:therapy*; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D006885:Hydroxybutyrates; D011091:Polyesters; D000091402:Prohibitins; C000717332:Phb protein, rat; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates