title: Influence of the period-dependent circadian clock on diurnal, circadian, and aperiodic gene expression in Drosophila melanogaster.
abstract: We measured daily gene expression in heads of control and period mutant Drosophila by using oligonucleotide microarrays. In control flies, 72 genes showed diurnal rhythms in light-dark cycles; 22 of these also oscillated in free-running conditions. The period gene significantly influenced the expression levels of over 600 nonoscillating transcripts. Expression levels of several hundred genes also differed significantly between control flies kept in light-dark versus constant darkness but differed minimally between per(01) flies kept in the same two conditions. Thus, the period-dependent circadian clock regulates only a limited set of rhythmically expressed transcripts. Unexpectedly, period regulates basal and light-regulated gene expression to a very broad extent.
authors.affiliation: Department of Genetics, Washington University Medical School, St. Louis, MO, 63110, USA.
authors.forename: Yiing
authors.initials: Y
authors.lastname: Lin
authors.forename: Mei
authors.initials: M
authors.lastname: Han
authors.forename: Brian
authors.initials: B
authors.lastname: Shimada
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Therese M
authors.initials: TM
authors.lastname: Gibler
authors.forename: Aloka
authors.initials: A
authors.lastname: Amarakone
authors.forename: Tarif A
authors.initials: TA
authors.lastname: Awad
authors.forename: Gary D
authors.initials: GD
authors.lastname: Stormo
authors.forename: Russell N
authors.initials: RN
authors.lastname: Van Gelder
authors.forename: Paul H
authors.initials: PH
authors.lastname: Taghert
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology* / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D020869:Gene Expression Profiling; D017344:Genes, Insect / Q000528:radiation effects; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D056950:Period Circadian Proteins; D017440:Photoperiod
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; C539040:PER protein, Drosophila; D056950:Period Circadian Proteins