title: Legume grains enhance ileal losses of specific endogenous serine-protease proteins in weaned pigs.
abstract: Feeding legume grains to pigs usually increases losses of endogenous proteins at the terminal ileum. However, the identity of such proteins is largely unknown. This study was undertaken to determine the ileal flow and identity of soluble proteins present in large concentrations in ileal digesta of young pigs fed soybean meal (SBM), peas (P), faba beans (FB), or blue lupin (L) in expt. 1, and white (WPC) or black (BPC) chickpeas in expt. 2. Protein in the control diet (C) was provided by casein. Ileal digesta proteins were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis, Coomassie blue staining, densitometry and N-terminal amino acid sequencing. Three protein bands at molecular masses of 25, 27, and 30 kDa had a higher ileal flow (P < 0.05) in the pigs fed the legume-based diets compared to those fed the control diet in expt. 2. This was true for the 25- and 30-kDa proteins (P < 0.05) and the 27-kDa protein (P < 0.10) in pigs fed the legume-containing diets in expt. 1. These proteins shared N-terminal amino acid sequences with enzymes of the serine protease family including pig trypsin (25 kDa) and blood coagulation factor IX or chymotrypsin (27 and 30 kDa).
authors.affiliation: Unité Mixte de Recherche sur le Veau et le Porc, INRA-ENSA de Rennes, 35042 Rennes Cedex, France.
authors.forename: Paulo
authors.initials: P
authors.lastname: Salgado
authors.forename: Lucile
authors.initials: L
authors.lastname: Montagne
authors.forename: João P B
authors.initials: JP
authors.lastname: Freire
authors.forename: Ricardo B
authors.initials: RB
authors.lastname: Ferreira
authors.forename: Artur
authors.initials: A
authors.lastname: Teixeira
authors.forename: Ofélia
authors.initials: O
authors.lastname: Bento
authors.forename: Manuel C
authors.initials: MC
authors.lastname: Abreu
authors.forename: René
authors.initials: R
authors.lastname: Toullec
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Lallès
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D000596:Amino Acids / Q000032:analysis / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D015153:Blotting, Western; D001835:Body Weight; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae; D005766:Gastrointestinal Contents / Q000737:chemistry; D005772:Gastrointestinal Transit; D007082:Ileum / Q000378:metabolism*; D008297:Male; D010940:Plant Proteins / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism*; D012995:Solubility; D013552:Swine; D014886:Weaning
chemical_list: D000596:Amino Acids; D010940:Plant Proteins; D012697:Serine Endopeptidases