Immunohistological detection of BRAF25 in human prostate tumor and cancer specimens.
BRAF25 is an alternatively spliced protein of BRAF35 (see associated paper). We have mapped the BRAF25 gene to chromosome sub-band 19p13.3, a region where loss of chromosomal heterozygosity has been reported in about 50% of ovarian cancers. Because of the high incidence of genetic links of prostate cancer to breast and ovarian cancers, we investigated the BRAF25 expression in the prostate specimens. Immunohistochemical analysis using antibodies specific for BRAF25 revealed a strong immunostaining in sections of the benign prostatic hyperplasia (BPH). The staining was concentrated on the nuclei of cells facing the lumen of prostatic glands, even though the sporadic nuclei of cells in stromas were also stained. However, the expression of BRAF25 was dramatically reduced in intermediate prostate cancer and absent in advanced prostate cancer. Preincubation of the antibody with the immunizing peptide abolished immunostaining in BPH specimens. Therefore, the expression of BRAF25 was gradually lost in prostate cancer.
Wang
Chiang
C
Department of Urology, University of Tennessee, Memphis, TN 38163, USA. cwang@utmem.edu
McCarty
Ida M
IM
Balazs
Louisa
L
Li
Yi
Y
Steiner
Mitchell S
MS
D000906:Antibodies / Q000276:immunology; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D006609:High Mobility Group Proteins; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D010455:Peptides / Q000276:immunology; D011470:Prostatic Hyperplasia / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D000906:Antibodies; D004268:DNA-Binding Proteins; C000625244:HMG20B protein, human; D006609:High Mobility Group Proteins; D010455:Peptides