title: Hypersensitivity to mosquito bites with natural-killer cell lymphocytosis: the possible implication of Epstein-Barr virus reactivation.
abstract: Hypersensitivity to mosquito bites (HMB) has been recognized as one of the wide variety of Epstein-Barr virus (EBV)-associated lymphoproliferative disorders, some of which overlap with chronic active EBV infection and hydroa vacciniforme. It frequently predisposes to hemophagocytic syndrome or lymphoid malignancy with a clonal EBV replication. Here we report an 8-year-old Japanese boy who presented with a 3-year history of HMB. Every summer, he had repeated severe local skin reactions with high-grade fever, lymphadenopathy, and mild liver dysfunction after mosquito bites. Histologically, lymphoid cells overexpressing EBV-encoded small nuclear RNAs (EBER), a marker of latent EBV infection, and eosinophils infiltrated diffusely into the lesional skin. His blood tests showed a marked increase in the CD11b+ 16+ 56+ natural-killer cell population (~ 50% of the peripheral lymphocytes), but neither a clonal proliferation of EBV nor abnormal antibody titers to EBV. Quantitative PCR using a cloned EBV-determined nuclear antigen (EBNA)-1 vector revealed that a high copy number of the EBV genome is integrated into his peripheral blood cells even during the mosquito-free winter. Based on this evidence, he was diagnosed as being in a smoldering stage of HMB, in which EBV-associated NK cell lymphoproliferation might participate in its pathogenesis.
authors.affiliation: Department of Dermatology, Fukushima Medical University School of Medicine, 1-Hikarigaoka, Fukushima 960-1295, Japan. masataka@fmu.ac.jp
authors.forename: Masataka
authors.initials: M
authors.lastname: Satoh
authors.forename: Noritaka
authors.initials: N
authors.lastname: Oyama
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Akiba
authors.forename: Mikio
authors.initials: M
authors.lastname: Ohtsuka
authors.forename: Keiji
authors.initials: K
authors.lastname: Iwatsuki
authors.forename: Fumio
authors.initials: F
authors.lastname: Kaneko
mesh_terms: D000818:Animals; D002648:Child; D009033:Culicidae; D020031:Epstein-Barr Virus Infections / Q000150:complications* / Q000473:pathology; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology* / Q000821:virology; D007299:Insect Bites and Stings / Q000150:complications* / Q000276:immunology; D007694:Killer Cells, Natural; D008297:Male; D016133:Polymerase Chain Reaction