RNA interference in adult mice.
RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.
McCaffrey
Anton P
AP
Department of Pediatrics, Stanford University School of Medicine, Stanford, California 94305-5208, USA.
Meuse
Leonard
L
Pham
Thu-Thao T
TT
Conklin
Douglas S
DS
Hannon
Gregory J
GJ
Kay
Mark A
MA
D000375:Aging / Q000235:genetics*; D000818:Animals; D020868:Gene Silencing; D017930:Genes, Reporter / Q000235:genetics; D016174:Hepacivirus / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering; D022661:RNA, Untranslated / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013379:Substrate Specificity; D019076:Transgenes / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase
D008156:Luciferases; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D022661:RNA, Untranslated; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase