Chemical tests as alternatives to animal tests in research on late symptoms in diabetes.
Glycation reactions, such as those seen in late diabetes, can be mimicked in purely chemical systems. The glycation is time-dependent, and in in vitro systems it can continue for days. Ascorbate seems to enhance the reactions. The reactions are associated with free-radical formation through transformation of an Amadori product to (deoxy-)glycoson, catalysed by heavy metals. Ascorbate enhances the reaction by a factor of 5-10 compared with in vitro systems without ascorbate. In vitro systems containing bovine serum albumin retard the formation of free-radicals, because of the formation of advanced glycation products.
Nielsen
Mette
M
Department of Life Sciences and Chemistry, Roskilde University, 400 Roskilde, Denmark.
Clausen
Jørgen
J
D000826:Animal Testing Alternatives / Q000379:methods*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D003920:Diabetes Mellitus; D005947:Glucose / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006031:Glycosylation; D017665:Hydroxyl Radical; D010084:Oxidation-Reduction
D017127:Glycation End Products, Advanced; D017665:Hydroxyl Radical; D005947:Glucose; D001205:Ascorbic Acid