title: Prolyl 4-hydroxylase inhibitor is more effective for the inhibition of proliferation than for inhibition of collagen synthesis of rat hepatic stellate cells.
abstract: The aim of this study was to investigate whether a prolyl 4-hydroxylase inhibitor (HOE 077) prevents the proliferation and collagen synthesis of rat hepatic stellate cells (HSCs). Rat HSCs were isolated and cultured with 100, 500, 1000 or 2000 &mgr;g/ml of HOE 077 with or without hepatocytes. After 4 day culture, the cell cycle of HSCs was examined by flow cytometry along with messenger RNA expression of procollagen type I. After 4 days of culture, HSCs had DNA synthesis and a high concentration (2000 &mgr;g/ml) of HOE 077 significantly reduced this DNA synthesis. However, HOE 077 incubated with hepatocytes could significantly reduce mitosis at a lower concentration of HOE077 (500 &mgr;g/ml) without significant reduction of alpha(2)(I) procollagen mRNA expression. These results indicate that the prolyl 4-hydroxylase inhibitor is more effective for the inhibition of proliferation than for inhibition of collagen synthesis of HSCs.
authors.affiliation: Department of Gastroenterology and Hepatology, School of Medicine, Yamaguchi University, Minami Kogushi 1-1-1, Ube, 755-8505, Yamaguchi, Japan
authors.forename: Mariko
authors.initials: M
authors.lastname: Aoyagi
authors.forename: Isao
authors.initials: I
authors.lastname: Sakaida
authors.forename: Chieko
authors.initials: C
authors.lastname: Suzuki
authors.forename: Makoto
authors.initials: M
authors.lastname: Segawa
authors.forename: Yohei
authors.initials: Y
authors.lastname: Fukumoto
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Okita