title: High Expression of Human Persephin in Insect Cells.
abstract: The human persephin (PSP) was expressed in Tn-5B1-4 insect cells using the Bac to Bac baculovirus expression system. The expressed product amounted for 20% of total cellular soluble proteins. The expressed product was purified by Ni(2+) affinity chromatography and the activity assays showed that it could significantly prolong the survival of spinal cord neurons.
authors.affiliation: Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. xfwu@sunm.shcnc.ac.cn
authors.forename: Zhe-Yu
authors.initials: ZY
authors.lastname: Chen
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: He
authors.forename: Cheng
authors.initials: C
authors.lastname: He
authors.forename: Chang-Lin
authors.initials: CL
authors.lastname: Lu
authors.forename: Xiang-Fu
authors.initials: XF
authors.lastname: Wu