title: Phosphatidyl ethanolamine is essential for targeting the arginine transporter Can1p to the plasma membrane of yeast.
abstract: In continuation of our previous study, we show that phosphatidyl ethanolamine (PE) depletion affects, in addition to amino acid transporters, activities of at least two other proton motive force (pmf)-driven transporters (Ura4p and Mal6p). For Can1p, we demonstrate that the lack of PE results in a failure of the permease targeting to plasma membrane. Despite the pleiotropic effect of PE depletion, a specific role of PE in secretion of a defined group of permeases can be distinguished. Pmf-driven transporters are more sensitive to the lack of PE than other plasma membrane proteins.
authors.affiliation: Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic. opekaro@biomed.cas.cz
authors.forename: Miroslava
authors.initials: M
authors.lastname: Opekarová
authors.forename: Ingrid
authors.initials: I
authors.lastname: Robl
authors.forename: Widmar
authors.initials: W
authors.lastname: Tanner
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism*; D026923:Amino Acid Transport Systems, Basic; D001120:Arginine / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D051246:Glucose Transport Proteins, Facilitative; D008364:Mannosyltransferases; D008565:Membrane Proteins / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D018892:Proton-Motive Force; D006180:Proton-Translocating ATPases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D033921:Vesicular Transport Proteins
chemical_list: D026905:Amino Acid Transport Systems; D026923:Amino Acid Transport Systems, Basic; C097640:CAN1 protein, Candida albicans; D005656:Fungal Proteins; D051246:Glucose Transport Proteins, Facilitative; C090594:HXT1 protein, S cerevisiae; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D010714:Phosphatidylethanolamines; C077883:SHR3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D033921:Vesicular Transport Proteins; C400810:ura4 protein, S pombe; C043909:arginine permease; D001120:Arginine; C090211:ANP1 protein, S cerevisiae; D008364:Mannosyltransferases; C092477:PMA1 protein, S cerevisiae; D006180:Proton-Translocating ATPases