Purification and initial crystallization studies of a DnaB intein from Synechocystis sp. PCC 6803.
A 154-residue mini-intein from the dnaB gene of Synechocystis sp. PCC 6803 (Ssp DnaB intein) has been purified and crystallized using PEG 4000 as a precipitant. The crystal belongs to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 58.2, c = 70.3 A. It has one molecule per asymmetric unit and diffracts to beyond 2.0 A under cryoconditions (100 K) using a rotating copper anode X-ray generator.
Chen
Xuehui
X
Laboratory of Structural Biology, Department of Biological Science and Technology and MOE Laboratory of Protein Science, Tsinghua University, Beijing 100084, People's Republic of China.
Xu
Ming Qun
MQ
Ding
Yi
Y
Ferrandon
Sébastien
S
Rao
Zihe
Z
D001426:Bacterial Proteins; D003300:Copper; D018360:Crystallography, X-Ray / Q000379:methods*; D000458:Cyanobacteria / Q000737:chemistry*; D004265:DNA Helicases / Q000737:chemistry* / Q000302:isolation & purification; D053488:DnaB Helicases; D004566:Electrodes; D004591:Electrophoresis, Polyacrylamide Gel; D010957:Plasmids / Q000378:metabolism; D013054:Spectrophotometry, Atomic; D013696:Temperature
D001426:Bacterial Proteins; D003300:Copper; D004265:DNA Helicases; D053488:DnaB Helicases