[Quantitative analysis on platelets cytosolic free calcium concentration and ulcerous tissue calcium in patients with recurrent aphthous ulceration].
To study the relationship between the abnormal behavior of calcium (Ca2+) and the pathogenesis of recurrent aphthous ulceration (RAU) by measuring quantitatively the concentration of platelets' cytosolic free calcium ions ([Ca2+]i) and tissue calcium (Wca2+), the patients with RAU (n = 50) and the healthy volunteers (n = 30) were chosen randomly. Then platelets [Ca2+]i was determined in calcium fluorescent indicator Fura-2/AM loaded washed platelets by spectrofluorophotometer and tissue Wca2+ was measured quantitatively by OCPC autoanalyzer method. The results showed that the concentration levels of platelets [Ca2+]i in patients with RAU in attack stage and attack-free stage were significantly higher than that of the control group (P < 0.01, P < 0.05), and the concentration level of the ulcerous tissue Wca2+ was also obviously higher than that of the normal control group (P < 0.001). There was a significantly positive correlation between the platelets [Ca2+]i and the ulcerous tissue Wca2+ (P < 0.01). The results suggested that the influx disorder and overload of Ca2+ might play an important role in the pathogenesis of RAU.
Huang
S
S
Department of Stomatology, Second Clinical College, Hunan Medical University.
Lin
T
T
Wu
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011897:Random Allocation; D013281:Stomatitis, Aphthous / Q000378:metabolism*
D002118:Calcium