Microbial production of extra-cellular phytase using polystyrene as inert solid support.
Aspergillus ficuum TUB F-1165 and Rhizopus oligosporus TUB F-1166 produced extra-cellular phytase during solid-state fermentation (SSF) using polystyrene as inert support. Maximal enzyme production (10.07 U/g dry substrate (U/gds) for A. ficuum and 4.52 U/gds for R. oligosporus) was observed when SSF was carried out with substrate pH 6.0 and moisture 58.3%, incubation temperature 30 degrees C, inoculum size of 1.3 x 10(7) spores/5 g substrate, for 72 h for A. ficuum and with substrate pH 7.0 and moisture 58.3%, incubation temperature 30 degrees C, inoculum size of 1 x 10(6) spores/5 g substrate for 96 h for R. oligosporus. Results indicated scope for production of phytase using polystyrene as inert support.
Gautam
Pinky
P
Biotechnology Division, Council of Scientific and Industrial Research, Trivandrum, India.
Sabu
A
A
Pandey
Ashok
A
Szakacs
George
G
Soccol
Carlos R
CR
D010832:6-Phytase / Q000096:biosynthesis*; D001230:Aspergillus / Q000145:classification / Q000378:metabolism*; D018441:Biofilms; D002244:Carbon / Q000378:metabolism; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009930:Organic Chemicals / Q000378:metabolism; D011137:Polystyrenes / Q000378:metabolism*; D012233:Rhizopus / Q000145:classification / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013379:Substrate Specificity; D013696:Temperature; D014867:Water / Q000378:metabolism
D009930:Organic Chemicals; D011137:Polystyrenes; D014867:Water; D002244:Carbon; D010832:6-Phytase