title: P450 BM3: the very model of a modern flavocytochrome.
abstract: Flavocytochrome P450 BM3 is a bacterial P450 system in which a fatty acid hydroxylase P450 is fused to a mammalian-like diflavin NADPH-P450 reductase in a single polypeptide. The enzyme is soluble (unlike mammalian P450 redox systems) and its fusion arrangement affords it the highest catalytic activity of any P450 mono-oxygenase. This article discusses the fundamental properties of P450 BM3 and how progress with this model P450 has affected our comprehension of P450 systems in general.
authors.affiliation: Department Biochemistry, University of Leicester, The Adrian Building, University Road, Leicester LE1 7RH, UK. awm9@le.ac.uk
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Munro
authors.forename: David G
authors.initials: DG
authors.lastname: Leys
authors.forename: Kirsty J
authors.initials: KJ
authors.lastname: McLean
authors.forename: Ker R
authors.initials: KR
authors.lastname: Marshall
authors.forename: Tobias W B
authors.initials: TW
authors.lastname: Ost
authors.forename: Simon
authors.initials: S
authors.lastname: Daff
authors.forename: Caroline S
authors.initials: CS
authors.lastname: Miles
authors.forename: Stephen K
authors.initials: SK
authors.lastname: Chapman
authors.forename: Dominikus A
authors.initials: DA
authors.lastname: Lysek
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Moser
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Page
authors.forename: P Leslie
authors.initials: PL
authors.lastname: Dutton
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000378:metabolism*; D004579:Electron Transport / Q000502:physiology*; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D005810:Multigene Family; D009251:NADPH-Ferrihemoprotein Reductase; D010084:Oxidation-Reduction; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases