Antioxidant activity of ethanolic extracts of amaranth seeds.
Antioxidant activity of ethanolic extracts obtained from two amaranth species was evaluated in a beta-carotene-linoleic acid model system. The addition of amaranth extracts in the range of 0.01-0.1% inhibited degradation of a beta-carotene in a model emulsion during incubation at 60 degrees C; 0.05% addition of amaranth seeds extract was proposed as practically applicable. The total content of phenolic compounds was estimated by the Folin-Ciocalteu method and ranged from 39.17 mg/100 g of Amaranthus caudatus to 56.22 mg/100 g of A. paniculatus seeds. Free phenolic acids contained in ethanolic extracts of amaranth seeds were purified and isolated by solid-phase extraction (SPE) and identified by reversed-phase high-performance liquid chromatography (RP-HPLC). The technique involved gave a good separation of the free phenolic acids in the amaranth seeds. Significant differences in phenolic acids profiles of both amaranth species were observed.
Klimczak
I
I
University of Economics, Faculty of Commodity Sciences, Al. Niepodleglosci 10, PL-60-967 Poznan, Poland. klimczak@novcil.ae.poznan.pl
Małecka
M
M
Pachołek
B
B
D027721:Amaranthus / Q000737:chemistry*; D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D013045:Species Specificity; D019207:beta Carotene / Q000037:antagonists & inhibitors / Q000378:metabolism
D000975:Antioxidants; D010936:Plant Extracts; D019207:beta Carotene; D000431:Ethanol