title: [Establishment of a method for detecting transforming growth factor beta 1 mRNA].
abstract: OBJECTIVE
  To establish a method to measure the TGF-beta 1 mRNA level for studying the mechanism of fibrogenesis caused by schistosomiasis japonica.
  
  
  METHODS
  Reverse-transcription polymerase chain reaction and dot blot analysis were used. A plasmid of TGF-beta 1 was constructed for standardization, and beta-actin was used as control. Eight different concentrations of the plasmid and 11 double-tube of TGF-beta 1 mRNA in the peripheral blood mononuclear cell (PBMC) of different persons were measured.
  
  
  RESULTS
  Quantitative results of 8 different dilutions of TGF-beta 1 plasmid had positive with the logarithm of the original concentration. The results of the 11 double-tube were 1.71 +/- 0.90 and 1.54 +/- 0.88.
  
  
  CONCLUSIONS
  The duplicability and stability of the method showed it can be used to analyse the TGF-beta 1 mRNA level of the peripheral blood mononuclear cells.
authors.affiliation: Institute of Infectious Diseases, Zhejiang Medical University, Hangzhou 310003.
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Cai
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Liu
mesh_terms: D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D012333:RNA, Messenger / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000097:blood* / Q000235:genetics; D053773:Transforming Growth Factor beta1
chemical_list: D012333:RNA, Messenger; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1