Interaction between potyvirus helper component-proteinase and capsid protein in infected plants.
Monoclonal antibodies were raised against helper component-proteinase (HcPro) purified from plants infected with the potyvirus Lettuce mosaic virus (LMV). These antibodies were used in a two-site triple antibody sandwich ELISA assay together with polyclonal antibodies directed against purified virions. An interaction between HcPro and the viral coat protein (CP) was demonstrated in extracts of LMV-infected leaves, as well as for two other potyviruses, Plum pox virus and Potato virus Y. The CP-HcPro interaction was not abolished in LMV derivatives with an HcPro GFP N-terminal fusion, or with a deletion from the CP of the amino acids involved in aphid transmission. Electron microscopy indicated that HcPro probably does not interact with the CP in the form of assembled virions or virus-like particles. Together, these results suggest that the interaction detected between CP and HcPro might be involved in a process of the potyvirus cycle different from aphid transmission.
Roudet-Tavert
Geneviève
G
Virologie Végétale, UMR GDPP, Institut de Biologie Végétale Moléculaire, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France1.
German-Retana
Sylvie
S
Virologie Végétale, UMR GDPP, Institut de Biologie Végétale Moléculaire, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France1.
Delaunay
Thierry
T
Virologie Végétale, UMR GDPP, Institut de Biologie Végétale Moléculaire, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France1.
Delécolle
Brigitte
B
Virologie Végétale, UMR GDPP, Institut de Biologie Végétale Moléculaire, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France1.
Candresse
Thierry
T
Virologie Végétale, UMR GDPP, Institut de Biologie Végétale Moléculaire, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France1.
Le Gall
Olivier
O
Virologie Végétale, UMR GDPP, Institut de Biologie Végétale Moléculaire, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France1.
D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D049452:Green Fluorescent Proteins; D018545:Lactuca / Q000378:metabolism* / Q000821:virology; D008164:Luminescent Proteins; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000378:metabolism*; D011485:Protein Binding; D020079:Terminal Repeat Sequences; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D008164:Luminescent Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus