Functional genomics of the endocrine pancreas: the pancreas clone set and PancChip, new resources for diabetes research.
Over the past 5 years, microarrays have greatly facilitated large-scale analysis of gene expression levels. Although these arrays were not specifically geared to represent tissues and pathways known to be affected by diabetes, they have been used in both type 1 and type 2 diabetes research. To prepare a tool that is particularly useful in the study of type 1 diabetes, we have assembled a nonredundant set of 3,400 clones representing genes expressed in the mouse pancreas or pathways known to be affected by diabetes. We have demonstrated the usefulness of this clone set by preparing a cDNA glass microarray, the PancChip, and using it to analyze pancreatic gene expression from embryonic day 14.5 through adulthood in mice. The clone set and corresponding array are useful resources for diabetes research.
Scearce
L Marie
LM
Department of Genetics, University of Pennsylvania, 415 Curie Boulevard, Philadephia, PA 19104, USA.
Brestelli
John E
JE
McWeeney
Shannon K
SK
Lee
Catherine S
CS
Mazzarelli
Joan
J
Pinney
Deborah F
DF
Pizarro
Angel
A
Stoeckert
Christian J
CJ
Clifton
Sandra W
SW
Permutt
M Alan
MA
Brown
Juliana
J
Melton
Douglas A
DA
Kaestner
Klaus H
KH
D000328:Adult; D003001:Cloning, Molecular; D003920:Diabetes Mellitus / Q000235:genetics*; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D023281:Genomics; D006801:Humans; D007231:Infant, Newborn; D007515:Islets of Langerhans / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics*; D012107:Research Design
D011506:Proteins