title: Chromosome analysis of blastocysts cultured under the diabetic condition.
abstract: Chromosomes of Slc:ICR mouse blastocysts cultured under the diabetic condition were analyzed to clarify the effect of glucose and ketone body (DL-beta-hydroxybutyric acid). In the group exposed to glucose plus ketone body or glucose alone, blastocysts showed higher incidences of chromosome abnormalities, especially numerical abnormalities such as aneuploidy and polyploidy, than in the control group (p < 0.01). The association of nucleolus organizing regions was increased in the blastocysts exposed to glucose plus ketone body, which seems to be related to the increase in numerical abnormalities. Structural abnormalities such as break and fragment were also observed, but there was no significant difference between the diabetic and nondiabetic conditions. These results from chromosome analysis of the cultured blastocysts suggest that the diabetic condition may directly cause chromosome abnormalities in early embryos, especially aneuploidies, and may thus induce duplications or deletions of genes. These chromosomal damages may disrupt the developmental programs for organogenesis and may be involved in diabetes-induced teratogenesis.
authors.affiliation: Department of Biology, Shimane Medical University, Izumo 693-8501, Japan. tatewak@shimane-med.ac.jp.
authors.forename: Reiko
authors.initials: R
authors.lastname: Tatewaki
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kagohashi
authors.forename: Kosuke
authors.initials: K
authors.lastname: Furuse
authors.forename: Hiroki
authors.initials: H
authors.lastname: Otani
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000494:pharmacology; D000782:Aneuploidy; D000818:Animals; D001755:Blastocyst / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure; D003920:Diabetes Mellitus / Q000235:genetics*; D004622:Embryo, Mammalian / Q000648:ultrastructure; D005947:Glucose / Q000494:pharmacology*; D007657:Ketone Bodies / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D011123:Polyploidy
chemical_list: D007657:Ketone Bodies; D005947:Glucose; D020155:3-Hydroxybutyric Acid