Single nucleotide polymorphisms in the transcobalamin gene: relationship with transcobalamin concentrations and risk for neural tube defects.
Homocysteine levels are elevated in mothers of neural tube defect (NTD) children, which may be due to a disturbed folate or vitamin B12 metabolism. Vitamin B12 is transported to the tissues by transcobalamin (TC). We previously showed that a low holo-TC/total-TC ratio is a risk factor for NTD, possibly due to an impaired binding of vitamin B12 to TC. The coding region of the TC gene of 12 individuals was analysed for genetic variations responsible for a disturbed vitamin B12 binding. The influence of the genetic variations observed on total-TC, holo-TC, holo-TC/total-TC, erythrocyte vitamin B12, plasma homocysteine concentrations and risk for NTD was explored in 42 mothers of a child with NTD and in 73 female controls. Direct sequencing analyses revealed five single nucleotide polymorphisms (SNPs). Three SNPs affected total-TC concentrations, whereas two SNPs seem to affect the binding of vitamin B12. None of the genotypes defined by the SNPs had a significant effect on homocysteine levels, or was associated with an increased NTD risk. Among the five SNPs observed only P259R could partly explain the reduced proportion of vitamin B12 bound to TC, which has been associated with an increased risk for having a child with NTD. Some of the variants studied affected total-TC and holo-TC/total-TC ratio but a larger study population is required to elucidate whether these SNPs influence delivery of vitamin B12 to the tissue, influence homocysteine levels and whether they are associated with an increased NTD risk.
Afman
Lydia A
LA
University Medical Center Nijmegen, Department of Pediatrics, Laboratory of Pediatrics and Neurology, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
Lievers
Karin J A
KJ
van der Put
Nathalie M J
NM
Trijbels
Frans J M
FJ
Blom
Henk J
HJ
D000328:Adult; D019943:Amino Acid Substitution; D005260:Female; D005787:Gene Frequency; D006801:Humans; D009426:Netherlands; D009436:Neural Tube Defects / Q000235:genetics*; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D014155:Transcobalamins / Q000235:genetics* / Q000378:metabolism*; D014805:Vitamin B 12 / Q000378:metabolism; D044465:White People
D014155:Transcobalamins; D014805:Vitamin B 12