Characterization of Salmonella serovars by PCR-single-strand conformation polymorphism analysis.
PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR-single-strand conformation polymorphism (PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the serovars could be achieved by this technique. However, PCR-SSCP analysis of the groEL genes of various Salmonella serovars produced 14 SSCP profiles, indicating the potential of this technique to differentiate different Salmonella serovars (interserovar differentiation). Moreover, PCR-SSCP could differentiate strains within a subset of serovars (intraserovar discrimination), as three SSCP profiles were produced for the 11 Salmonella enterica serovar Enteritidis strains, and two SSCP profiles were generated for the 7 S. enterica serovar Infantis and five S. enterica serovar Newport strains. PCR-SSCP has the potential to complement classical typing methods such as serotyping and phage typing for the typing of Salmonella serovars due to its rapidity, simplicity, and typeability.
Nair
Satheesh
S
Institute of Postgraduate Studies & Research, University of Malaya, Kuala Lumpur, Malaysia. h0sathe@yahoo.com
Lin
Thong Kwai
TK
Pang
Tikki
T
Altwegg
Martin
M
D015373:Bacterial Typing Techniques; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D012475:Salmonella / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012480:Salmonella Infections / Q000175:diagnosis / Q000382:microbiology; D012703:Serotyping; D013045:Species Specificity
D018834:Chaperonin 60; D004269:DNA, Bacterial