title: Analysis of the hematopoietic potential of muscle-derived cells in mice.
abstract: Cells in murine muscle have been reported to differentiate into hematopoietic stem and progenitor cells and thus repopulate the hematopoietic system of an irradiated animal. This activity was attributed to muscle stem cells. We used an in vitro and in vivo approach to identify the hematopoietic repopulating activity found in muscle tissue of mice by antibody staining and cell sorting. We confirmed existence of a hematopoietic repopulating cell in muscle tissue, but the data strongly suggest that repopulation is due not to muscle stem cells but to hematopoietic cells present in muscle tissue. Unexpectedly, the blood-forming cells were enriched in muscle relative to their frequency in peripheral blood.
authors.affiliation: Department of Internal Medicine, University of Kentucky Medical Center, Lexington, KY 40536, USA. ghart2@uky.edu
authors.forename: Hartmut
authors.initials: H
authors.lastname: Geiger
authors.forename: Jarrod M
authors.initials: JM
authors.lastname: True
authors.forename: Barry
authors.initials: B
authors.lastname: Grimes
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Carroll
authors.forename: Roger A
authors.initials: RA
authors.lastname: Fleischman
authors.forename: Gary
authors.initials: G
authors.lastname: Van Zant
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D016130:Immunophenotyping; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology