Immunocytochemical expression of microtubule-associated protein-2 (MAP-2) in small cell lung cancer cell lines with neuronal-like processes.
Five of six human small cell lung cancer (SCLC) cell lines changed morphologically into cells with neuronal-like processes on the extracellular matrix of human lung adenocarcinoma cell line PC-9 cells (PC-9 / ECM substrate). The features of the neuronal-like processes of these SCLC cell lines were examined immunocytochemically using monoclonal antibodies against beta-chains of tubulin and microtubule-associated protein-2 (MAP-2), which is somatodendritic MAP of neurons. It was observed that beta-chains of tubulin and MAP-2 were expressed along the neuronal-like processes of SCLC cell lines. These findings suggest that the beta-chains of tubulin and MAP-2 are expressed functionally in SCLC cell lines in association with the development of dendrite-like processes on PC-9 / ECM substrate.
Tanaka
Kazuhiko
K
Radiobiology, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045. ktanaka@ncc.go.jp
Terasaki
Takeo
T
D018288:Carcinoma, Small Cell / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D011233:Precipitin Tests; D014404:Tubulin / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D008869:Microtubule-Associated Proteins; D014404:Tubulin; D000242:Cyclic AMP