[Sample preparation for electron microscope with tissues fixed with formalin and embedded in paraffin].
A method of sample preparation for electron microscope is introduced. After paraffin-embedded tissues, fresh and fixed on time, were deparaffined, the tissue were fixed in 4% glutaraldehyde for 24 hours, in 1% osmic acid for 1-2 hours, and dehydrated with acetone and embedded in Epon812; then they were cut into ultrathin sections. By this method, better ultrastructure of paraffin-embedded tissues was obtained, and the method could satisfy the demand for observation under electron microscope. In addition, some important proceedings were also discussed in the paper.
Jing
J
J
Electron Microscope Laboratory, Hunan Medical University, Changsha 410078.
D005557:Formaldehyde; D006801:Humans; D007414:Intestinal Neoplasms / Q000648:ultrastructure*; D016612:Paraffin Embedding; D013048:Specimen Handling; D016707:Tissue Fixation
D005557:Formaldehyde