Successful boosting of a DNA measles immunization with an oral plant-derived measles virus vaccine.
Despite eradication attempts, measles remains a global health concern. Here we report results that demonstrate that a single-dose DNA immunization followed by multiple boosters, delivered orally as a plant-derived vaccine, can induce significantly greater quantities of measles virus-neutralizing antibodies than immunization with either DNA or plant-derived vaccines alone. This represents the first demonstration of an enhanced immune response to a prime-boost vaccination strategy combining a DNA vaccine with edible plant technology.
Webster
Diane E
DE
Department of Medicine, Monash University Medical School, Alfred Hospital, Prahran, Victoria 3181, Australia.
Cooney
Michelle L
ML
Huang
Zhongjun
Z
Drew
Damien R
DR
Ramshaw
Ian A
IA
Dry
Ian B
IB
Strugnell
Richard A
RA
Martin
Jenny L
JL
Wesselingh
Steve L
SL
D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000276:immunology*; D007117:Immunization, Secondary; D008457:Measles / Q000517:prevention & control*; D008458:Measles Vaccine / Q000276:immunology*; D008459:Measles virus / Q000276:immunology; D051379:Mice; D009500:Neutralization Tests; D030821:Plants, Genetically Modified / Q000276:immunology*; D014026:Nicotiana / Q000235:genetics; D019444:Vaccines, DNA / Q000276:immunology*
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D008458:Measles Vaccine; D019444:Vaccines, DNA; C032397:hemagglutinin protein G, measles virus