title: Serum leptin concentrations after surgery in young rats.
abstract: OBJECTIVE
  We describe changes in serum leptin concentrations after surgery (laparotomy, partial hepatectomy, splenectomy, and unilateral nephrectomy) in young male rats. Because we presumed that pain and inflammation would influence food intake and subsequent leptin release, laparotomized and partially hepatectomized rats were treated with ibuprofen.
  
  
  METHODS
  Preoperative blood samples were taken from the retroorbital sinuses, and postoperative blood samples were taken from the aorta, vena portae, and vena cava 18 h after surgery. Serum leptin concentrations were estimated by radioimmunoassay.
  
  
  RESULTS
  Pre- and postoperative serum leptin concentrations did not differ significantly in rats with partial hepatectomy (5.1 +/- 0.4 versus 4.2 +/- 1.1 microg/L), splenectomy (6.1 +/- 0.4 versus 5.0 +/- 0.6 microg/L), or unilateral nephrectomy (4.1 +/- 0.7 versus 5.0 +/- 1.3 microg/L). Significant decreases in serum leptin were observed after laparotomy (8.1 +/- 0.9 versus 3.7 +/- 0.5 microg/L), laparotomy plus ibuprofen treatment (8.9 +/- 1.6 versus 3.1 +/- 0.4 microg/L), and partial hepatectomy plus ibuprofen treatment (5.4 +/- 0.6 versus 3.2 +/- 0.3 microg/L). We observed no significant differences in serum leptin concentrations measured in different regions of the body.
  
  
  CONCLUSIONS
  Surgical interventions in young rats are accompanied by decreases in serum leptin. The liver, spleen, and kidney may participate in leptin clearance. Ibuprofen treatment leads to additional decreases in serum leptin concentrations.
authors.affiliation: Institute of Physiology, Charles University Prague, Faculty of Medicine and Faculty Hospital, CZ 5005 Hradec Kralove, Czech Republic. zivny@lfhk.cuni.cz
authors.forename: Helena
authors.initials: H
authors.lastname: Zivna
authors.forename: Pavel
authors.initials: P
authors.lastname: Zivny
authors.forename: Vladimir
authors.initials: V
authors.lastname: Palicka
mesh_terms: D000311:Adrenal Glands / Q000033:anatomy & histology; D000818:Animals; D004435:Eating; D006498:Hepatectomy; D007052:Ibuprofen / Q000627:therapeutic use; D007668:Kidney / Q000033:anatomy & histology; D007813:Laparotomy; D020738:Leptin / Q000097:blood*; D008099:Liver / Q000033:anatomy & histology; D008297:Male; D009392:Nephrectomy; D009929:Organ Size; D011184:Postoperative Period; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000033:anatomy & histology; D013156:Splenectomy; D013514:Surgical Procedures, Operative
chemical_list: D020738:Leptin; D007052:Ibuprofen