title: Expression of Human Epiregulin in E.coli.
abstract: Human epiregulin cDNA was amplified from the lung cancer cell line A549 using RT-PCR. After adding 6 His codon to its 3' end, it was cloned into a high efficient secretive Escherichia coli system with alkaline phosphatase promoter(phoA promoter)constructed in our lab and induced for expression. The product was purified one-step by Ni-NTA column. Amino acid sequence analysis revealed the identity of our product with that previously reported. The product showed strong proliferative effect on fibroblast cell line Balb/c3T3 and growth inhibitory effect on epithelial carcinoma cell line A431.
authors.affiliation: Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. zpli@sunm.shcnc.ac.cn
authors.forename: Quan-Sheng
authors.initials: QS
authors.lastname: Xi
authors.forename: Xin-Guo
authors.initials: XG
authors.lastname: Qian
authors.forename: Qing-Wei
authors.initials: QW
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Pan
authors.forename: Zai-Ping
authors.initials: ZP
authors.lastname: Li
authors.forename: Ren-Bao
authors.initials: RB
authors.lastname: Gan