title: Identification of Protein Spots in Silver-stained Two-dimensional Gels by MALDI-TOF Mass Peptide Map Analysis.
abstract: Protein spots in silver-stained two dimensional gels were analyzed and identified by employing an improved procedure of mass spectrometric peptide mapping, including i) In-gel reduction, alkylation and enzymatic digestion ii) Extraction and desalting by using a pipette tip containing a small C18 micro-column (ZipTip(TM)) iii) Direct MALDI-TOF mass analysis and protein database searching. The results demonstrate that single silver-stained protein spots in a 2-DE-gel could be identified rapidly by this procedure and the use of the ZipTip(TM) pipette tip could increase evidently the sensitivity of the MALDI-TOF analysis. By using this the procedure, 10 protein spots in a silver-stained gel of 2-DE of crude venom of the spider S.Huwena were analyzed and identified.
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, China. Liangsp@public.cs.hn.cn
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Jing-Yun
authors.initials: JY
authors.lastname: Xie
authors.forename: Song-Ping
authors.initials: SP
authors.lastname: Liang