Study on the components of luobuma with peroxynitrite-scavenging activity.
The origin of the antioxidant activity of Luobuma aqueous extract was examined by measuring the peroxynitrite (ONOO-)-eliminating activities of fractions of this extract obtained by Sephadex LH-20 column chromatography. Three of the four fractions obtained, i.e., those excluding the H2O-eluted fraction, were found to possess (ONOO-)-eliminating activity. These three fractions were combined and fractionated again by Sephadex LH-20 column chromatography, which yielded five fractions. Seven different compounds were isolated from two of these five fractions with high activity. Epigallocatechin-(4beta-8)-epicatechin showed the highest (ONOO-)-eliminating activity.
Yokozawa
Takako
T
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Japan. yokozawa@ms.toyama-mpu.ac.jp
Kashiwada
Yoshiki
Y
Hattori
Masao
M
Chung
Hae Young
HY
D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology*; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D030421:Peroxynitrous Acid / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013439:Sulfhydryl Reagents; D013455:Sulfur / Q000737:chemistry
D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D010936:Plant Extracts; D013439:Sulfhydryl Reagents; D030421:Peroxynitrous Acid; D013455:Sulfur; D002392:Catechin; C057580:gallocatechol