Cloning an iron-regulated metal transporter from rice.
Rice cDNA and genomic libraries were screened in order to clone an Fe(II) transporter gene. A cDNA clone highly homologous to the Arabidopsis Fe(II) transporter gene IRT1 was isolated from Fe-deficient rice roots. The cDNA clone was named OsIRT1. A genomic clone corresponding to the cDNA was also obtained, sequenced and analysed. When expressed in yeast cells, OsIRT1 cDNA reversed the growth defects of the yeast iron-uptake mutant. Northern blot analysis revealed that OsIRT1 mRNA was predominantly expressed in roots and was induced by Fe- and Cu-deficiency. This suggests that OsIRT1 is a functional metal transporter for iron, and is actively engaged in Fe uptake from soils, especially under limiting conditions.
Bughio
Naimatullah
N
Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, 113-8657 Tokyo, Japan.
Yamaguchi
Hirotaka
H
Nishizawa
Naoko K
NK
Nakanishi
Hiromi
H
Mori
Satoshi
S
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003300:Copper / Q000172:deficiency; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D007501:Iron / Q000493:pharmacokinetics; D000090463:Iron Deficiencies; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D010940:Plant Proteins; D018749:RNA, Plant; D003300:Copper; D007501:Iron