title: Structural and genetic analysis of the BldB protein of Streptomyces coelicolor.
abstract: We have demonstrated that the bldB gene of Streptomyces coelicolor is required for the formation of aerial hyphae and the synthesis of antibiotics. We also found that BldB forms a higher-order complex (most likely a dimer) and that amino acid residues 20 to 78 are important for this interaction. This region is conserved in the BldB family, suggesting that dimer formation may be a common feature of these proteins.
authors.affiliation: Antimicrobial Research Centre and Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5.
authors.forename: Marcus
authors.initials: M
authors.lastname: Eccleston
authors.forename: Reem Ahmed
authors.initials: RA
authors.lastname: Ali
authors.forename: Richard
authors.initials: R
authors.lastname: Seyler
authors.forename: Janet
authors.initials: J
authors.lastname: Westpheling
authors.forename: Justin
authors.initials: J
authors.lastname: Nodwell
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000235:genetics / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017353:Gene Deletion; D016680:Genome, Bacterial; D019077:Helix-Turn-Helix Motifs; D025301:Hyphae / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010641:Phenotype; D016415:Sequence Alignment; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C111307:BldB protein, Streptomyces coelicolor