title: Surface engineering of poly(DL-lactic acid) by entrapment of alginate-amino acid derivatives for promotion of chondrogenesis.
abstract: Alginate-amino acid derivatives were explored to engineer poly(DL-lactic acid)(PDL-LA) as glycocalyx-like surface to promote cell adhesion and growth. Four different kinds of alginate-amino acid derivatives were synthesized to mimic the glycocalyx of cell membrane to promote chondrogenesis. The alginate-amino acid derivatives were characterized by FT-IR, 1H NMR and UV spectra and the amino acid content on alginate-amino acid derivatives was given by ninhydrin-UV method. A new strategy, entrapment, was then employed to modify PDL-LA membranes with alginate and its amino acid derivatives. The results of XPS, ATR-FTIR and contact angle confirmed that a stable thin film of alginate and its amino acid derivatives can be entrapped on the surface of PDL-LA membrane. The chondrocyte cytocompatibility test and MTT assays indicated that the alginate-amino acid derivatives modified PDL-LA membranes could promote chondrogenesis. The novel surface treatment method may have potentials for tissue engineering and other biomedical applications.
authors.affiliation: Department of Polymer Science, Zhejiang University, Hangzhou, China.
authors.forename: Huiguang
authors.initials: H
authors.lastname: Zhu
authors.forename: Jian
authors.initials: J
authors.lastname: Ji
authors.forename: Rongyi
authors.initials: R
authors.lastname: Lin
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
authors.forename: Linxian
authors.initials: L
authors.lastname: Feng
authors.forename: Jiacong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D019344:Lactic Acid / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D000464:Alginates; D000596:Amino Acids; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D020723:Glucuronic Acid