title: Determination of the structure of a decay accelerating factor-binding clinical isolate of echovirus 11 allows mapping of mutants with altered receptor requirements for infection.
abstract: We have used X-ray crystallography to determine the structure of a decay accelerating factor (DAF)-binding, clinic-derived isolate of echovirus 11 (EV11-207). The structures of the capsid proteins closely resemble those of capsid proteins of other picornaviruses. The structure allows us to interpret a series of amino acid changes produced by passaging EV11-207 in different cell lines as highlighting the locations of multiple receptor-binding sites on the virion surface. We suggest that a DAF-binding site is located at the fivefold axes of the virion, while the binding site for a distinct but as yet unidentified receptor is located within the canyon surrounding the virion fivefold axes.
authors.affiliation: Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom.
authors.forename: Amanda D
authors.initials: AD
authors.lastname: Stuart
authors.forename: Thomas A
authors.initials: TA
authors.lastname: McKee
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Williams
authors.forename: Chris
authors.initials: C
authors.lastname: Harley
authors.forename: Shuo
authors.initials: S
authors.lastname: Shen
authors.forename: David I
authors.initials: DI
authors.lastname: Stuart
authors.forename: T David K
authors.initials: TD
authors.lastname: Brown
authors.forename: Susan M
authors.initials: SM
authors.lastname: Lea
mesh_terms: D000818:Animals; D001665:Binding Sites; D018958:CD55 Antigens / Q000378:metabolism*; D002213:Capsid / Q000648:ultrastructure; D036022:Capsid Proteins; D002522:Chlorocebus aethiops; D018360:Crystallography, X-Ray; D029822:Enterovirus B, Human / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity* / Q000648:ultrastructure*; D019073:HT29 Cells; D006801:Humans; D009154:Mutation; D011991:Receptors, Virus / Q000378:metabolism*; D014709:Vero Cells; D014764:Viral Proteins / Q000648:ultrastructure; D014771:Virion / Q000648:ultrastructure
chemical_list: D018958:CD55 Antigens; D036022:Capsid Proteins; D011991:Receptors, Virus; C065897:VP1 protein, echovirus 11; D014764:Viral Proteins; C074958:echovirus 11 receptor