Dietary genistein affects brain protein synthesis rates in ovariectomized female rats.
The purpose of this study was to determine whether genistein affects the rate of brain protein synthesis in ovariectomized female rats. Experiments were conducted on three groups of 12-wk-old female rats: those in group 1 were ovariectomized to reduce the level of plasma sex hormone; those in group 2 were ovariectomized and fed diets containing 0.01% genistein; and those in group 3 were sham-operated controls. The fractional rates of protein synthesis in the brain of ovariectomized rats fed genistein were significantly greater than those in ovariectomized rats without genistein treatment. In the cerebral cortex and cerebellum, the RNA activity [g protein synthesized/(g RNA.d)] significantly correlated (r > 0.86, P < 0.001) with the fractional rate of protein synthesis. The RNA concentration (mg RNA/g protein) was not related to the fractional rate of protein synthesis in any organ. The results suggest that the addition of genistein to the diet of ovariectomized female rats is likely to increase the rate of protein synthesis in the brain, and that RNA activity is at least partly related to the fractional rate of brain protein synthesis.
Lyou
Sunok
S
Department of Home Economics, Aichi University of Education, Kariya, Japan.
Hirano
Emi
E
Tujioka
Kazuyo
K
Mawatari
Yuka
Y
Hayase
Kazutoshi
K
Okuyama
Satoshi
S
Yokogoshi
Hidehiko
H
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D004032:Diet; D005260:Female; D019833:Genistein / Q000008:administration & dosage* / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D010052:Ovariectomy; D012313:RNA / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D014018:Tissue Distribution
D009419:Nerve Tissue Proteins; D012313:RNA; D019833:Genistein