title: Structural hierarchy of several proteins observed by wide-angle solution scattering.
abstract: In the present study using a high-intensity X-ray beam from a third-generation synchrotron radiation source, it is demonstrated that a wide-angle X-ray scattering (WAXS) profile from several globular proteins in solution can reflect not only the overall structures (approximately 300 A distance resolution) but also intramolecular structures ranging to secondary structures (approximately 2.5 A distance resolution). The proteins treated in the present experiments are classified as different types of structure categories, namely, as all-alpha, all-beta and alpha + beta proteins. Here the full-range experimental scattering curves are compared with the theoretical curves, suggesting a further availability of the SR-WAXS method for studies of structure hierarchy and the function of proteins in solutions.
authors.affiliation: Department of Physics, Gunma University, Maebashi 371-8510, Japan. mhirai@fs.aramaki.gunma-u.ac.jp
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Hirai
authors.forename: Hiroki
authors.initials: H
authors.lastname: Iwase
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Keiko
authors.initials: K
authors.lastname: Miura
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Inoue
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry; D011487:Protein Conformation; D012542:Scattering, Radiation; D017356:Synchrotrons / Q000295:instrumentation
chemical_list: D009211:Myoglobin; C421864:hen egg lysozyme; D009113:Muramidase