Hyposialylation of integrins stimulates the activity of myeloid fibronectin receptors.
Despite numerous reports suggesting that beta(1) integrin receptors undergo differential glycosylation, the potential role of N-linked carbohydrates in modulating integrin function has been largely ignored. In the present study, we find that beta(1) integrins are differentially glycosylated during phorbol ester (PMA)-stimulated differentiation of myeloid cells along the monocyte/macrophage lineage. PMA treatment of two myeloid cell lines, U937 and THP-1, induces a down-regulation in expression of the ST6Gal I sialyltransferase. Correspondingly, the beta(1) integrin subunit becomes hyposialylated, suggesting that the beta(1) integrin is a substrate for this enzyme. The expression of hyposialylated beta(1) integrin isoforms is temporally correlated with enhanced binding of myeloid cells to fibronectin, and, importantly, fibronectin binding is inhibited when the Golgi disrupter, brefeldin A, is used to block the expression of the hyposialylated form. Consistent with the observation that cells with hyposialylated integrins are more adhesive to fibronectin, we demonstrate that the enzymatic removal of sialic acid residues from purified alpha(5)beta(1) integrins stimulates fibronectin binding by these integrins. These data support the hypothesis that unsialylated beta(1) integrins are more adhesive to fibronectin, although desialylation of alpha(5) subunits could also contribute to increased fibronectin binding. Collectively our results suggest a novel mechanism for regulation of the beta(1) integrin family of cell adhesion receptors.
Semel
Alexis C
AC
Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama, 35294, USA.
Seales
Eric C
EC
Singhal
Anuj
A
Eklund
Elizabeth A
EA
Colley
Karen J
KJ
Bellis
Susan L
SL
D000581:Amidohydrolases / Q000378:metabolism; D015153:Blotting, Western; D020126:Brefeldin A / Q000494:pharmacology; D002241:Carbohydrates / Q000737:chemistry; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000378:metabolism; D022423:Myeloid Cells / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D010703:Phorbol Esters / Q000378:metabolism; D011485:Protein Binding; D017469:Receptors, Fibronectin / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism*; D013997:Time Factors; D020298:U937 Cells
D002241:Carbohydrates; D005353:Fibronectins; D037102:Lectins; D010703:Phorbol Esters; D017469:Receptors, Fibronectin; D012794:Sialic Acids; D020126:Brefeldin A; C119222:phorbol-12-myristate; D009439:Neuraminidase; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase