title: Ploidy levels among species in the 'Oxalis tuberosa alliance' as inferred by flow cytometry.
abstract: The 'Oxalis tuberosa alliance' is a group of Andean Oxalis species allied to the Andean tuber crop O. tuberosa Molina (Oxalidaceae), commonly known as 'oca'. As part of a larger project studying the origins of polyploidy and domestication of cultivated oca, flow cytometry was used to survey DNA ploidy levels among Bolivian and Peruvian accessions of alliance members. In addition, this study provided a first assessment of C-values in the alliance by estimating nuclear DNA contents of these accessions using chicken erythrocytes as internal standard. Ten Bolivian accessions of cultivated O. tuberosa were confirmed to be octoploid, with a mean nuclear DNA content of approx. 3.6 pg/2C. Two Peruvian wild Oxalis species, O. phaeotricha and O. picchensis, were inferred to be tetraploid (both with approx. 1.67 pg/2C), the latter being one of the putative progenitors of O. tuberosa identified by chloroplast-expressed glutamine synthetase data in prior work. The remaining accessions (from 78 populations provisionally identified as 35 species) were DNA diploid, with nuclear DNA contents varying from 0.79 to 1.34 pg/2C.
authors.affiliation: The Field Museum of Natural History, Botany Department, Chicago, Illinois 60606-2496, USA. eemshwiller@fieldmuseum.org
authors.forename: Eve
authors.initials: E
authors.lastname: Emshwiller
mesh_terms: D001838:Bolivia; D002875:Chromosomes / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D004171:Diploidy; D019143:Evolution, Molecular; D005434:Flow Cytometry / Q000379:methods*; D019684:Magnoliopsida / Q000145:classification* / Q000235:genetics*; D010568:Peru; D010802:Phylogeny; D011123:Polyploidy; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D018744:DNA, Plant