title: Characterization of cloned mouse Na+/taurocholate cotransporting polypeptide by transient expression in COS-7 cells.
abstract: The mouse Na+/taurocholate cotransporting polypeptide transiently expressed in COS-7 cells caused sodium-dependent uptake of [3H]taurocholic acid with Km and Vmax values of 18 microM and 102 pmol/mg protein/min, respectively. This Km value is comparable to that for rat NTCP and higher than that for human NTCP. Substrate specificity was evaluated by measuring inhibitory effects of unlabeled bile acids on [3H]taurocholic acid transport.
authors.affiliation: Department of Biological Resource Chemistry, Faculty of Agriculture, Kyoto Prefectural University, Japan. tsaeki@kpu.ac.jp
authors.forename: Tohru
authors.initials: T
authors.lastname: Saeki
authors.forename: Noriki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Ryuhei
authors.initials: R
authors.lastname: Kanamoto
authors.forename: Kimikazu
authors.initials: K
authors.lastname: Iwami
mesh_terms: D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D006801:Humans; D051379:Mice; D029363:Organic Anion Transporters, Sodium-Dependent; D051381:Rats; D027981:Symporters
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D029363:Organic Anion Transporters, Sodium-Dependent; D027981:Symporters; C071908:sodium-bile acid cotransporter