Monoclonal antibodies specific to yellow-head virus (YHV) of Penaeus monodon.
Monoclonal antibodies specific to 22, 67 and 135 kDa proteins of yellow-head virus (YHV) were produced from a mouse immunized with partially purified YHV isolated from the haemolymph of experimentally YHV-infected Penaeus monodon. Four groups of monoclonal antibodies were identified. One group of antibodies bound only to native protein of YHV while the others were specific to 135, 67 and 22 kDa proteins in both native and denatured forms. All antibodies could be used to detect YHV infection by means of dot blot and immunohistochemistry. However, antibodies specific to the 22 kDa protein gave the best immunohistochemistry results in terms of intensity and sharpness of staining.
Sithigorngul
Paisarn
P
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, Thailand. paisarn@psm.swu.ac.th
Rukpratanporn
Sombat
S
Longyant
Siwaporn
S
Chaivisuthangkura
Parin
P
Sithigorngul
Weerawan
W
Menasveta
Piamsak
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006458:Hemolymph / Q000276:immunology; D006825:Hybridomas; D015151:Immunoblotting / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D051379:Mice; D008970:Molecular Weight; D028381:Nidovirales / Q000276:immunology* / Q000302:isolation & purification; D033561:Penaeidae / Q000276:immunology / Q000821:virology*; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014764:Viral Proteins