Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli.
A yedU gene product with a molecular mass of 31 kDa is a hypothetical protein with no known function. The protein was purified and crystallized at 296 K. X-ray diffraction data have been collected to 2.3 A using synchrotron radiation. The crystals belong to the primitive orthorhombic system, with unit-cell parameters a = 50.56, b = 63.45, c = 168.02 A. The asymmetric unit contains two monomers of the protein, with a corresponding V(M) of 2.25 A(3) Da(-1) and a solvent content of 44.84%.
Kim
Ok-Gwan
OG
Beamline Division, Pohang Accelerator Laboratory, Pohang, Kyungbuk 790-784, South Korea.
Kim
In-Kwon
IK
Kim
Ghyung-Hwa
GH
Ko
Junsang
J
Park
Chankyu
C
Suh
Pann-Ghill
PG
Kang
Sa-Ouk
SO
Lee
Heung-Soo
HS
Cha
Sun-Shin
SS
D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D018832:Molecular Chaperones / Q000737:chemistry*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D017356:Synchrotrons
D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D011994:Recombinant Proteins; C469996:hchA protein, E coli