Genome expression analysis of Anopheles gambiae: responses to injury, bacterial challenge, and malaria infection.
The complex gene expression responses of Anopheles gambiae to microbial and malaria challenges, injury, and oxidative stress (in the mosquito and/or a cultured cell line) were surveyed by using cDNA microarrays constructed from an EST-clone collection. The expression profiles were broadly subdivided into induced and down-regulated gene clusters. Gram+ and Gram- bacteria and microbial elicitors up-regulated a diverse set of genes, many belonging to the immunity class, and the response to malaria partially overlapped with this response. Oxidative stress activated a distinctive set of genes, mainly implicated in oxidoreductive processes. Injury up- and down-regulated gene clusters also were distinctive, prominently implicating glycolysis-related genes and citric acid cycle/oxidative phosphorylation/redox-mitochondrial functions, respectively. Cross-comparison of in vivo and in vitro responses indicated the existence of tightly coregulated gene groups that may correspond to gene pathways.
Dimopoulos
George
G
Department of Biological Sciences, Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, London SW7 2AZ, United Kingdom.
Christophides
George K
GK
Meister
Stephan
S
Schultz
Jörg
J
White
Kevin P
KP
Barillas-Mury
Carolina
C
Kafatos
Fotis C
FC
D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000469:parasitology; D018076:DNA, Complementary; D005260:Female; D016678:Genome; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D010961:Plasmodium / Q000472:pathogenicity*
D018076:DNA, Complementary