Prognostic value of quantitative Kaposi sarcoma--associated herpesvirus load in posttransplantation Kaposi sarcoma.
Organ transplant recipients have a higher risk of Kaposi sarcoma (KS). A quantitative real-time polymerase chain reaction assay was developed to evaluate KS-associated herpesvirus (KSHV) as a prognostic tool in transplant recipients with KS. Forty-three patients who developed KS after transplantation were included in a cross-sectional study to correlate virus load with transplantation or KS parameters. Seventeen patients (40%) had KSHV viremia (>100 copies/microg of DNA; median, 6067 copies/microg of DNA). Factors associated with these levels of viremia by univariate analysis were progression of KS (P=.00002), time from KS diagnosis (P=.0007), actual stage of KS (P=.006), initial stage of KS (P=.22), graft loss (P=.013), and time from transplantation (P=.0246). Disease progression remained associated with KSHV viremia in a multivariate analysis (P=.01). Thus, quantification of KSHV load in peripheral blood mononuclear cells could represent a useful tool for monitoring transplant recipients with KS.
Pellet
Claire
C
Laboratory of Pharmacology, Hôpital Saint-Louis, Paris, France.
Chevret
Sylvie
S
Francès
Camille
C
Euvrard
Sylvie
S
Hurault
Mylène
M
Legendre
Christophe
C
Dalac
Sophie
S
Farge
Dominique
D
Antoine
Corinne
C
Hiesse
Christian
C
Peraldi
Marie-Noëlle
MN
Lang
Philippe
P
Samuel
Didier
D
Calmus
Yvon
Y
Agbalika
Félix
F
Morel
Patrice
P
Calvo
Fabien
F
Lebbé
Céleste
C
D015331:Cohort Studies; D003430:Cross-Sectional Studies; D004279:DNA, Viral; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016030:Kidney Transplantation; D009000:Monocytes / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D011183:Postoperative Complications; D011379:Prognosis; D012514:Sarcoma, Kaposi / Q000473:pathology / Q000821:virology*; D019562:Viral Load; D014766:Viremia / Q000821:virology*
D004279:DNA, Viral