Kinetic fluorimetric determination of the antineoplastic methotrexate (MTX) in human serum.
A kinetic study of the oxidation of methotrexate (MTX) in acidic medium and in the presence of potassium permanganate has been made on the basis of the fluorescence-time curves. A kinetic method for the determination of MTX was developed with a range of application between 0.22 and 3.30 microM. The proposed kinetic method permits us to determine MTX in human serum and to avoid the natural fluorescence of the serum. A detection limit of 0.18 microM was calculated in the presence of ascorbic acid as activator. Only 100 s per sample is necessary for the analysis. The interference of pteridin derivatives and the rescue agent folinic acid (leucovorin) was tested.
Espinosa-Mansilla
A
A
Department of Analytical Chemistry, University of Extremadura, 06071, Badajoz, Spain. nuncy@unex.es
Durán Merás
I
I
Zamora Madera
A
A
Pedano
L
L
Ferreyra
C
C
D000964:Antimetabolites, Antineoplastic / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008727:Methotrexate / Q000097:blood*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000964:Antimetabolites, Antineoplastic; D008727:Methotrexate