PUVA-induced apoptosis involves mitochondrial dysfunction caused by the opening of the permeability transition pore.
The mechanism of cell death was investigated in Jurkat cells exposed to the combination of psoralen and UVA irradiation (PUVA). Apoptosis was by far prevailing over necrosis and involved mitochondrial dysfunction. The collapse of mitochondrial membrane potential, appears to be caused by the opening of the mitochondrial permeability transition pore since its inhibitor, cyclosporin A, prevented mitochondrial dysfunction and largely attenuated apoptosis. Apoptosis also occurred in cells treated with the photoproducts generated by irradiating psoralen in vitro with an oxygen-dependent process. Thus, the involvement of reactive oxygen species in the onset of PUVA-induced apoptosis appears mostly related to psoralen photooxidation.
Canton
Marcella
M
Dipartimento di Chimica Biologica, Università di Padova, Viale Giuseppe Colombo 3, Italy.
Caffieri
Sergio
S
Dall'Acqua
Francesco
F
Di Lisa
Fabio
F
D017209:Apoptosis; D005363:Ficusin / Q000494:pharmacology*; D006801:Humans; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D007473:Ion Channels / Q000037:antagonists & inhibitors*; D019169:Jurkat Cells; D008564:Membrane Potentials / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D014466:Ultraviolet Rays
D007473:Ion Channels; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D017319:Photosensitizing Agents; D011065:Poly(ADP-ribose) Polymerases; D005363:Ficusin