title: Effectiveness of photodynamic ablation for destruction of endometrial explants in a rat endometriosis model.
abstract: OBJECTIVE
  To evaluate the potential of photodynamic therapy with aminolevulinic acid (ALA-PDT) for ablation of endometrial explants in a rat endometriosis model and to compare the effect of ALA-PDT, electrosurgery, and surgical resection on normal peritoneum.
  
  
  METHODS
  Prospective controlled experimental trial.
  
  
  METHODS
  University medical center.
  
  
  METHODS
  Mature Sprague-Dawley female rats.
  
  
  METHODS
  Induction of endometriosis and subsequent treatment with ALA-PDT; electrosurgery, and simple resection, and ALA-PDT of normal peritoneum.
  
  
  METHODS
  Histopathological assessment.
  
  
  RESULTS
  Systemic ALA followed by exposure to photoactivating light for 10 or 15 minutes resulted in ablation of all explants harvested 3-4 days after treatment. Permanent destruction was confirmed by absence of regrowth by week 3. Exposure of normal peritoneum to ALA-PDT resulted in initial necrosis, with complete recovery by day 16. Adhesions were present on day 16 in 50% of cases after electrosurgery and in 100% of cases after resection. No adhesions were present in ALA-PDT-treated animals.
  
  
  CONCLUSIONS
  Systemic ALA followed by exposure to photoactivating light at relatively low power densities for periods as brief as 10 minutes resulted in ablation of endometriotic explants. Exposure of normal peritoneum to ALA-PDT resulted in complete resurfacing. Both electrosurgery and surgical resection resulted in a greater incidence of surface adhesions.
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynaecology, Kingston, Ontario, Canada.
authors.forename: Alicja A
authors.initials: AA
authors.lastname: Krzemien
authors.forename: Dean A
authors.initials: DA
authors.lastname: Van Vugt
authors.forename: William A
authors.initials: WA
authors.lastname: Fletcher
authors.forename: Robert L
authors.initials: RL
authors.lastname: Reid
mesh_terms: D000622:Aminolevulinic Acid / Q000627:therapeutic use*; D000818:Animals; D004598:Electrosurgery / Q000009:adverse effects; D004715:Endometriosis / Q000188:drug therapy* / Q000473:pathology / Q000601:surgery; D005260:Female; D015994:Incidence; D010532:Peritoneal Diseases / Q000453:epidemiology / Q000209:etiology; D010537:Peritoneum / Q000187:drug effects / Q000601:surgery; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D011183:Postoperative Complications; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D000267:Tissue Adhesions / Q000453:epidemiology / Q000209:etiology
chemical_list: D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid