Determination of superoxide dismutase and glutathione peroxidase activities in blood of healthy pediatric subjects.
BACKGROUND
Given the growing requirement of antioxidant enzymes measurements in laboratory and the increasing role of SOD/GPx ratio in the balance of reactive oxygen species (ROS), the aim of our study was to contribute to define reference values of enzyme activities in Italian healthy children, by determining SOD in erythrocytes and GPx in whole blood.


METHODS
SOD (E.C.1.15.1.1) and GPx (E.C.1.11.1.9) activities were spectrophotometrically assayed in erythrocytes with commercial kits. SOD activity was expressed as the amount of protein causing a 50% inhibition of formazan dye (505 nm), employing xanthine and xanthine oxidase to generate superoxide radicals. Units of GPx activity were calculated following NADPH oxidation at 340 nm using cumene hydroperoxide as the substrate.


RESULTS
Superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were assayed in blood of 45 healthy pediatric subjects (26 males and 19 females between 0 and 14 years of age). We found no significant differences in blood antioxidant enzymes both in all population and when we distributed the subjects for age classes and sex.


CONCLUSIONS
the definition of pediatric values of antioxidant enzyme activities in blood of healthy children may be useful in monitoring SOD and GPx in physiologic and pathologic conditions, also for future therapeutic trials.
Gaeta
Laura Maria
LM
Molecular Medicine Unit, Children's Hospital Bambino Gesù, Piazza S. Onofrio, 4, 00165 Rome, Italy.
Tozzi
Giulia
G
Pastore
Anna
A
Federici
Giorgio
G
Bertini
Enrico
E
Piemonte
Fiorella
F
D000293:Adolescent; D000375:Aging / Q000097:blood; D002648:Child; D002675:Child, Preschool; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D006262:Health; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007558:Italy; D008297:Male; D012016:Reference Values; D012727:Sex Characteristics; D013482:Superoxide Dismutase / Q000097:blood*
D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase