title: Rats fed a high sucrose diet have altered heart antioxidant enzyme activity and gene expression.
abstract: Several studies in human and animal models have shown that consumption of fructose facilitates oxidative damage but the mechanisms involved are unclear. In this study, the effects of two weeks of high sucrose on both oxidative stress parameters and stress-related gene expression, using a cDNA array, were investigated in rat heart. Both increased TBARS and lower Cu-Zn-SOD activity were found in heart from high sucrose fed rats compared to rats on a starch diet. Higher plasma NO level was also found in the high sucrose group, corroborating the pro-oxidant effect of fructose. The Cu-Zn-SOD mRNA level was also greater in the high sucrose group; the Mn-SOD, GPX and catalase were not different between the two groups. Increased HSP70 and decreased COMT genes expression were observed, underlying the hypertensive effect of dietary fructose. These findings confirm the pro-oxidant effect of high sucrose feeding to rats and highlight the NO/O(2)(*-) balance importance in oxidative homeostasis.
authors.affiliation: Centre de Recherche en Nutrition Humaine d'Auvergne, Unité des Maladies Métaboliques et Micronutriments, INRA, Theix, 63122 Saint-Genès-Champanelle, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Busserolles
authors.forename: Wioletta
authors.initials: W
authors.lastname: Zimowska
authors.forename: Edmond
authors.initials: E
authors.lastname: Rock
authors.forename: Yves
authors.initials: Y
authors.lastname: Rayssiguier
authors.forename: Andrzej
authors.initials: A
authors.lastname: Mazur
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001835:Body Weight; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D019422:Dietary Sucrose / Q000008:administration & dosage*; D015870:Gene Expression; D020869:Gene Expression Profiling; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D009206:Myocardium / Q000201:enzymology* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D019422:Dietary Sucrose; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase