Isolation and culture conditions of a Klebsiella pneumoniae strain that can utilize ammonium and nitrate ions simultaneously with controlled iron and molybdate ion concentrations.
A bacterial strain F-5-2, isolated from soil and identified as Klebsiella pneumoniae, removed NH4+ completely in 24 h of aerobic cultivation in a medium containing 1 mg/ml of NH4NO3. However, 70% of the NO3- originally provided remained. When 100 microM Fe2+ was added to the medium, both NH4+ and NO3- were removed simultaneously and completely from the culture within 6 h of incubation. In addition, the amount of MoO4- in the medium markedly affected the bacterial cell growth and utilization of NH4+ and NO3-. The bacterium could remove 4 mg/ml of NH4NO3 completely in 48 h of aerobic cultivation in a medium containing 100 microM Fe2+ and 0.8 pM MoO4(2-). The total nitrogen in the culture containing its cells was decreased to 14% of that in the NH4NO3 originally provided. GC-MS analysis demonstrated that N2 was generated from the nitrogen atoms of both NH4+ and NO3-.
Kim
Young-Ju
YJ
Division of Life Science, The Graduate School of Science and Technology, Faculty of Agriculture, Kobe University, Rokko, Japan.
Yoshizawa
Makoto
M
Takenaka
Shinji
S
Murakami
Shuichiro
S
Aoki
Kenji
K
D008401:Gas Chromatography-Mass Spectrometry; D007501:Iron / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism; D008982:Molybdenum / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*
D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D008982:Molybdenum; D007501:Iron