Sequence analysis of F gene of SF02 isolate of goose paramyxovirus and its identification by multiplex RT-PCR.
The complete F gene of SF02 of goose paramyxovirus (GPV) has been cloned and analyzed. The sequence analysis demonstrated that the F gene of SF02 contains 1 662 nt and encodes 553 amino acids, and its cleavage activation site of F gene has the same deduced amino acid sequence, (112)R-R-Q-K-R-F(117), as the velogenic (highly pathogenic) strain of newcastle disease virus. The latter correlated with the virulence of the isolate in biological assays. The F gene of SF02 isolate with the domestic standard velogenic strain NDV, F48E9, shared 86.5% homology in nucleotide and 90.8% homology in amino acid sequences. The SF02 isolate is closer to some NDV strains prevalent in Taiwan and West-European countries in recent years. Based on the F gene sequence a multiplex RT-PCR method has been developed. It could be used for the discrimination of GPV from NDV.
Zou
Jian
J
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China. gongzx@sunm.shcnc.ac.cn
Shan
Song-Hua
SH
Yao
Long-Tao
LT
Gong
Zu-Xun
ZX
D000595:Amino Acid Sequence; D000818:Animals; D018109:Avulavirus / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005777:Geese / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014760:Viral Fusion Proteins / Q000235:genetics*
D018076:DNA, Complementary; D012367:RNA, Viral; D014760:Viral Fusion Proteins