Effect of immunization with plasmid DNA encoding for rinderpest virus matrix protein on systemic rinderpest virus infection in rabbits.
Plasmid vaccine pBK-CMVMPILC113 expressing the matrix (M) gene of rinderpest virus was assessed for its potential to protect rabbits against a lethal viral challenge. Rabbits immunized with plasmids expressing the M gene were not protected when challenged with lapinized rinderpest virus, despite the production of anti-M antibodies, while rabbits immunized with rinderpest tissue culture vaccine were completely protected from a lethal challenge with lapinized rinderpest virus. The plasmid vaccine also had no significant effect on the lymphopenia in challenged rabbits. The results indicate that rinderpest M protein does not have a protective role in rinderpest infection.
Pahar
B
B
National Project on Rinderpest Eradication, Division of Biochemistry and Food Sciences, Indian Veterinary Research Institute, Izatnagar. bpahar@primate.ucdavis.edu
Sharma
B
B
Goel
A C
AC
D000818:Animals; D015153:Blotting, Western; D001831:Body Temperature / Q000276:immunology; D001835:Body Weight / Q000276:immunology; D019556:COS Cells; D002522:Chlorocebus aethiops; D005260:Female; D007114:Immunization / Q000662:veterinary*; D018655:Lymphocyte Count / Q000662:veterinary; D008297:Male; D010957:Plasmids / Q000235:genetics / Q000276:immunology*; D011817:Rabbits / Q000276:immunology* / Q000821:virology; D012301:Rinderpest / Q000276:immunology* / Q000517:prevention & control; D012302:Rinderpest virus / Q000235:genetics / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology* / Q000592:standards; D014709:Vero Cells; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D019444:Vaccines, DNA; D014763:Viral Matrix Proteins; D014765:Viral Vaccines