Susceptibility testing of Aspergillus spp. by means of an automated blood culture system.
The BacT/Alert blood culture system was evaluated for the MIC determination of amphotericin B for 27 strains of Aspergillus from five different species. An inoculum of c. 10(8) cfu/mL and an incubation time of 48 h resulted in MICs for 26 of 27 strains that corresponded with published MICs obtained in a microdilution assay. Twelve of 13 strains with MIC(48) values >/=2 mg/L showed growth within 24 h in bottles containing 0.25 mg/L amphotericin B, indicating that results can be obtained the day following inoculation. It is concluded that the BacT/Alert blood culture system can be used for susceptibility testing of Aspergillus spp. with amphotericin B.
Rath
P-M
PM
Institut für Medizinische Mikrobiologie, Universitätsklinikum Essen, Hufelandstrasse 55, D-45122 Essen, Germany. rath@uni-essen.de
Freise
J M
JM
Ansorg
R
R
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects*; D001769:Blood / Q000382:microbiology*; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D012680:Sensitivity and Specificity; D015610:Serum Bactericidal Test / Q000379:methods*
D000935:Antifungal Agents; D000666:Amphotericin B