title: Development and optimization of a reversed-phase high-performance liquid chromatographic method for the determination of piperacillin and tazobactam in tazocin injectable powder.
abstract: A reversed phase high-performance liquid chromatographic method with detection at 220 nm was developed and validated for the determination of piperacillin, I, and tazobactam, II, in Tazocin injectable powder. Acetaminophen was used as internal standard. A Hypersil BDS RP-C(18) column (250 x 4.6 mm), 5 microm particle size, was equilibrated with a mobile phase composed of aqueous solution of sodium dihydrogenphosphate-dihydrate (20 mM)-acetonitrile-methanol (70:15:15, v/v/v) and pH 5.0. Its flow rate was 1.0 ml/min. Calibration curves were linear for I and II in the concentration ranges of 3.0 x 10(-7)-2.0 x 10(-4) M and 7.0 x 10(-7)-2.0 x 10(-4) M, respectively. Limits of detection and quantitation were 1 x 10(-7), 3 x 10(-7) M for I and 2 x 10(-7), 7 x 10(-7) M for II, respectively. Relative standard deviation, for I and II was less than 0.40 and 0.75%, respectively. Extensive recovery studies were also performed.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, 15771, Athens, Greece.
authors.forename: S C
authors.initials: SC
authors.lastname: Marselos
authors.forename: H A
authors.initials: HA
authors.lastname: Archontaki
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010316:Particle Size; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry*; D010878:Piperacillin / Q000032:analysis* / Q000737:chemistry*; D000077725:Piperacillin, Tazobactam Drug Combination; D011208:Powders; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D000078142:Tazobactam
chemical_list: D011208:Powders; D000077725:Piperacillin, Tazobactam Drug Combination; D010397:Penicillanic Acid; D000078142:Tazobactam; D010878:Piperacillin