Establishment of fluorescent lung carcinoma metastasis model and its real-time microscopic detection in SCID mice.
Lung cancer is the most prevalent malignant tumor in the world. Metastasis of the disease causes death in lung cancer patients. Recent study has shown that multiple cascades of gene defects occur in lung cancer. In this report, we established a novel H1299/EGFP tumor model to determine whether H1299 transfected with the enhanced green fluorescent protein (EGFP) gene in vitro and xenotransplanted into SCID mouse lung would permit the detection of lung cancer micrometastasis in vivo. We demonstrated that EGFP-transduced H1299 cells maintained stable high-level EGFP expressions during their growth in vivo. EGFP fluorescence clearly demarcated the primary seeding place and readily allowed for the visualization of distant micrometastasis and local invasion at the single-cell level. Small metastatic and locally invasive foci, including those immediately adjacent to the tumor's leading invasive edge, were almost undetectable by routine hematoxylin and eosin staining and immunohistochemistry. The GFP tagged lung cancer model is superior for the detection and study of physiologically relevant patterns of lung cancer invasion and metastasis in vivo.
Huang
Ming-Shyan
MS
Department of Internal Medicine, Kaohsiung Medical University, Taiwan.
Wang
Tzu-Jou
TJ
Liang
Chung-Ling
CL
Huang
Huey-Mei
HM
Yang
I-Chi
IC
Yi-Jan
Hua
H
Hsiao
Michael
M
D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology / Q000556:secondary*; D000328:Adult; D000818:Animals; D002277:Carcinoma / Q000737:chemistry*; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry / Q000473:pathology / Q000556:secondary*; D003199:Computer Systems; D005456:Fluorescent Dyes / Q000032:analysis*; D049452:Green Fluorescent Proteins; D006801:Humans; D007267:Injections; D008164:Luminescent Proteins / Q000032:analysis*; D008168:Lung; D008175:Lung Neoplasms / Q000737:chemistry / Q000473:pathology*; D051379:Mice; D016513:Mice, SCID; D008856:Microscopy, Fluorescence; D023421:Models, Animal; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000032:analysis*; D009368:Neoplasm Transplantation; D009928:Organ Specificity; D011993:Recombinant Fusion Proteins / Q000032:analysis; D014162:Transfection; D014183:Transplantation, Heterologous
D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins