title: Indirect measurement of the production of plasma triacylglycerols by horses given a high-fat diet.
abstract: The hypothesis tested was that the feeding of extra fat to horses would raise the production of plasma triacylglycerols (TAG). To measure TAG secretion, the indirect Triton method was used. Six adult horses were given a low-fat control or a high-fat diet according to a crossover design. In keeping with our earlier work, the high-fat diet lowered fasting plasma TAG concentrations by an average of 42% and raised post-heparin total lipoprotein lipase activity by 79%. The rate of increase in plasma TAG concentration after Triton administration was 49% lower when the horses were fed the high-fat diet instead of the low-fat control diet. Thus, the hypothesis is rejected. It is suggested that the dose of Triton used in the study might have been too low to fully depress lipoprotein lipase activity, leading to an outcome of the study that was opposite to that expected.
authors.affiliation: Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: Suzanne N J
authors.initials: SN
authors.lastname: Geelen
authors.forename: Marianne M
authors.initials: MM
authors.lastname: Sloet van Oldruitenborgh-Oosterbaan
authors.forename: Anton C
authors.initials: AC
authors.lastname: Beynen
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage*; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage; D005260:Female; D006736:Horses / Q000097:blood*; D008049:Lipase / Q000097:blood; D008071:Lipoprotein Lipase / Q000097:blood; D008099:Liver / Q000201:enzymology; D008297:Male; D012044:Regression Analysis; D014280:Triglycerides / Q000097:blood*
chemical_list: D004041:Dietary Fats; D005217:Fat Emulsions, Intravenous; D014280:Triglycerides; D008049:Lipase; D008071:Lipoprotein Lipase