title: Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor antibody.
abstract: Anti-idiotype antibodies (Ab2) play an important role in the homeostasis of immune responses and are related to the development and the disease activity of certain autoimmune diseases. The asialoglycoprotein receptor (ASGPR) is considered one of the target antigens in the pathogenesis of autoimmune chronic active hepatitis (AIH). We previously developed a mouse monoclonal antibody (clone 8D7) which recognizes rat and human ASGPR. In this study, to help investigate the anti-ASGPR antibody-anti-idiotype antibody network in patients with AIH, we developed a syngeneic mouse monoclonal Ab2 to the 8D7 anti-ASGPR antibody (Ab1). One clone, designated as 3C8, tested positive for specific reactivity to 8D7-Ab1 and did not bind to other irrelevant immunoglobulins. By competitive inhibition assays, the binding of 8D7-Ab1 to liver membrane extracts, i.e., the crude antigen preparation, was inhibited by 3C8-Ab2 in a dose-dependent manner, and the binding of 8D7-Ab1 to 3C8-Ab2 was inhibited by the liver membrane extracts. In the immunohistochemical analysis, 3C8-Ab2 blocked the specific staining of sinusoidal margins of rat hepatocytes by 8D7-Ab1. These results suggest that 3C8 anti-idiotype antibody recognizes the specific idiotypic determinants within the antigen-binding site of 8D7-Ab1.
authors.affiliation: Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry, Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Hirai
authors.forename: Motowo
authors.initials: M
authors.lastname: Mizuno
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Morisue
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshioka
authors.forename: Morizou
authors.initials: M
authors.lastname: Shimada
authors.forename: Junichirou
authors.initials: J
authors.lastname: Nasu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okada
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shimomura
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Takao
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D037263:Asialoglycoprotein Receptor / Q000276:immunology*; D019693:Hepatitis, Autoimmune / Q000276:immunology*; D022781:Hepatocytes / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D037263:Asialoglycoprotein Receptor