Green fluorescent protein-labeled recombinant fluobody for detecting the picloram herbicide.
A green fluorescent protein-labeled fluobody was designed to develop a simple immunoassay method for detecting picloram herbicide in an environmental sample. The gfp gene was successfully inserted into the pSJF2 vector harboring the picloram-specific antibody fragment to yield pSJF2GFP. Picloram spiking in an environmental river sample could be indirectly detected by observing the fluorescence intensity value of the gfp-fluobody, exhibiting specific sensitivity to free picloram with an IC50 value of 50 ppb. Using the gfp-fluobody immunoassay avoids the enzyme-substrate reaction for calorimetric detection that is required in an enzyme-linked immunosorbent assay (ELISA).
Kim
In Seon
IS
Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Canada. ikim@ualberta.ca
Shim
Jae Han
JH
Suh
Yong Tack
YT
Yau
Kerrm Y F
KY
Hall
J Christopher
JC
Trevors
Jack T
JT
Lee
Hung
H
D001483:Base Sequence; D017931:DNA Primers; D049452:Green Fluorescent Proteins; D006540:Herbicides / Q000032:analysis*; D007118:Immunoassay / Q000379:methods; D008164:Luminescent Proteins / Q000737:chemistry; D010846:Picloram / Q000032:analysis*; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D006540:Herbicides; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D010846:Picloram