Alternative sources of neurons and glia from somatic stem cells.
Stem cell populations have been shown to be extremely versatile: they can generate differentiated cells specific to the tissue in which they reside and descendents that are of different germ layer origin. This raises the possibility of obtaining neuronal cells from new biological source of the same adult human subjects. In this study, we found that epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) cooperated to induce the proliferation, self-renewal, and expansion of neural stem cell-like population isolated from several newborn and adult mouse tissues: muscle and hematopoietic tissues. This population, in both primary culture and secondary expanded clones, formed spheres of undifferentiated cells that were induced to differentiate into neurons, astrocytes, and oligodendrocytes. Brain engraftment of the somatic-derived neural stem cells generated neuronal phenotypes, demonstrating the great plasticity of these cells with potential clinical application.
Torrente
Yvan
Y
IRCCS Ospedale Maggiore Policlinico, Italy.
Belicchi
Marzia
M
Pisati
Federica
F
Pagano
Stefano F
SF
Fortunato
Francesco
F
Sironi
Manuela
M
D'Angelo
Maria Grazia
MG
Parati
Eugenio A
EA
Scarlato
Guglielmo
G
Bresolin
Nereo
N
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D019070:Cell Lineage; D002469:Cell Separation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism / Q000601:surgery; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005456:Fluorescent Dyes; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells / Q000166:cytology*; D009457:Neuroglia / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009930:Organic Chemicals
D005456:Fluorescent Dyes; D009930:Organic Chemicals; C070080:PKH 26; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor