Overlapping and unique roles for C-terminal binding protein 1 (CtBP1) and CtBP2 during mouse development.
The C-terminal binding protein (CtBP) family of proteins has been linked to multiple biological processes through their association with numerous transcription factors. We generated mice harboring mutations in both Ctbp1 and Ctbp2 to address the in vivo function of CtBPs during vertebrate development. Ctbp1 mutant mice are small but viable and fertile, whereas Ctbp2-null mice show defects in axial patterning and die by E10.5 due to aberrant extraembryonic development. Mice harboring various combinations of Ctbp1 and Ctbp2 mutant alleles exhibit dosage-sensitive defects in a wide range of developmental processes. The strong genetic interaction, as well as transcription assays with CtBP-deficient cells, indicates that CtBPs have overlapping roles in regulating gene expression. We suggest that the observed phenotypes reflect the large number of transcription factors whose activities are compromised in the absence of CtBP.
Hildebrand
Jeffrey D
JD
Program in Developmental Biology and Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98108, USA. jeffh@pitt.edu
Soriano
Philippe
P
D000015:Abnormalities, Multiple; D000429:Alcohol Oxidoreductases; D000483:Alleles; D017398:Alternative Splicing; D000818:Animals; D019521:Body Patterning / Q000502:physiology; D002460:Cell Line; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins / Q000502:physiology*; D004622:Embryo, Mammalian / Q000502:physiology; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D018390:Gene Targeting; D017930:Genes, Reporter; D005820:Genetic Testing; D017403:In Situ Hybridization; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010750:Phosphoproteins / Q000502:physiology*; D010920:Placenta / Q000002:abnormalities / Q000098:blood supply; D017386:Sequence Homology, Amino Acid; D013234:Stem Cells; D015017:Yolk Sac / Q000002:abnormalities / Q000098:blood supply
D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D000429:Alcohol Oxidoreductases; C111341:Ctbp2 protein, mouse; C096794:C-terminal binding protein