Complementary profiling of gene expression at the transcriptome and proteome levels in Saccharomyces cerevisiae.
Using an integrated genomic and proteomic approach, we have investigated the effects of carbon source perturbation on steady-state gene expression in the yeast Saccharomyces cerevisiae growing on either galactose or ethanol. For many genes, significant differences between the abundance ratio of the messenger RNA transcript and the corresponding protein product were observed. Insights into the perturbative effects on genes involved in respiration, energy generation, and protein synthesis were obtained that would not have been apparent from measurements made at either the messenger RNA or protein level alone, illustrating the power of integrating different types of data obtained from the same sample for the comprehensive characterization of biological systems and processes.
Griffin
Timothy J
TJ
Institute for Systems Biology, Seattle, Washington 98103, USA.
Gygi
Steven P
SP
Ideker
Trey
T
Rist
Beate
B
Eng
Jimmy
J
Hood
Leroy
L
Aebersold
Ruedi
R
D019992:Databases as Topic; D013058:Mass Spectrometry; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012333:RNA, Messenger / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*
D011506:Proteins; D012333:RNA, Messenger