Bismuth-induced lysosomal rupture in J774 cells.
Bismuth-containing drugs have several applications, one being their use against Helicobacter pylori-associated peptic ulcers, and bismuth has been discovered in macrophages at the base and margins of peptic ulcers. In the present study, the autometallographic technique for the histochemical demonstration of bismuth was applied, showing that bismuth citrate-exposed J774 cells accumulate the metal in their lysosomes. Such accumulations resulted in lysosomal rupture - assayed by the acridine orange uptake technique and flow cytofluorometry - and ensuing apoptotic cell death.
Stoltenberg
M
M
Department of Neurobiology, Institute of Anatomy, University of Aarhus, Denmark. ms@neuro.au.dk
Larsen
A
A
Zhao
M
M
Danscher
G
G
Brunk
U T
UT
D000165:Acridine Orange / Q000737:chemistry; D000818:Animals; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D008247:Lysosomes / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009942:Organometallic Compounds / Q000009:adverse effects / Q000493:pharmacokinetics* / Q000633:toxicity*; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D000165:Acridine Orange; C002791:bismuth tripotassium dicitrate