Structural and genetic analysis of the BldB protein of Streptomyces coelicolor.
We have demonstrated that the bldB gene of Streptomyces coelicolor is required for the formation of aerial hyphae and the synthesis of antibiotics. We also found that BldB forms a higher-order complex (most likely a dimer) and that amino acid residues 20 to 78 are important for this interaction. This region is conserved in the BldB family, suggesting that dimer formation may be a common feature of these proteins.
Eccleston
Marcus
M
Antimicrobial Research Centre and Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5.
Ali
Reem Ahmed
RA
Seyler
Richard
R
Westpheling
Janet
J
Nodwell
Justin
J
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000235:genetics / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017353:Gene Deletion; D016680:Genome, Bacterial; D019077:Helix-Turn-Helix Motifs; D025301:Hyphae / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010641:Phenotype; D016415:Sequence Alignment; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C111307:BldB protein, Streptomyces coelicolor