title: Processing of 3'-extended read-through transcripts by the exosome can generate functional mRNAs.
abstract: Strains carrying rna14.1 and rna15.2 mutations are defective in pre-mRNA 3' cleavage, polyadenylation, and transcription termination. Long extended read-through transcripts generated in rna14.1 and rna15.2 strains are greatly stabilized by depletion of Rrp41p, a core component of the exosome complex or the RNA helicase Dob1p/Mtr4p. The absence of the nuclear-specific exosome component, Rrp6p, from the rna14.1 strain gave a very different phenotype. Short polyadenylated pre-mRNAs were strongly stabilized, and these were functional for translation. Production of these mRNAs was suppressed by depletion of Rrp41p, indicating that they are the products of exosome processing followed by uncoupled polyadenylation. The balance between complete degradation of 3'-unprocessed pre-mRNAs and their processing to functional mRNAs is regulated, with degradation favored on glucose media.
authors.affiliation: Wellcome Trust Centre for Cell Biology, University of Edinburgh, EH9 3JR Edinburgh, Scotland, UK.
authors.forename: Claire
authors.initials: C
authors.lastname: Torchet
authors.forename: Cecile
authors.initials: C
authors.lastname: Bousquet-Antonelli
authors.forename: Laura
authors.initials: L
authors.lastname: Milligan
authors.forename: Emma
authors.initials: E
authors.lastname: Thompson
authors.forename: Joanna
authors.initials: J
authors.lastname: Kufel
authors.forename: David
authors.initials: D
authors.lastname: Tollervey
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism*; D063326:Exosome Multienzyme Ribonuclease Complex; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D008957:Models, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature
chemical_list: D020413:3' Untranslated Regions; D003470:Culture Media; D005656:Fungal Proteins; D046911:Macromolecular Substances; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D005095:Exoribonucleases; D063326:Exosome Multienzyme Ribonuclease Complex; C112610:RRP6 protein, S cerevisiae