A syntenin-like protein with postsynaptic density protein (PDZ) domains produced by black tiger shrimp Penaeus monodon in response to white spot syndrome virus infection.
We report the isolation and characterization of products from a subtractive cDNA library from the haemolymph of Penaeus monodon experimentally infected with white spot syndrome virus (WSSV). One cDNA derived from up-regulated mRNA was identified. A homology search indicated similarity to the putative protein syntenin (TE8). The nearly complete nucleotide sequence of TE8 was obtained by rapid amplification of cDNA (RACE). Its putative protein product contained a tandem repeat of PDZ domains (postsynaptic density protein or PSD-95, DlgA and ZO-1). We propose that TE8 may function as an adapter that couples PDZ-binding protein(s) in a signaling pathway involved in the shrimp response to WSSV.
Bangrak
Phuwadol
P
Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkla, Thailand.
Graidist
Potchanapond
P
Chotigeat
Wilaiwan
W
Supamattaya
Kidchakan
K
Phongdara
Amornrat
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D004267:DNA Viruses / Q000502:physiology*; D018076:DNA, Complementary / Q000032:analysis / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D005784:Gene Amplification; D015967:Gene Expression Regulation, Viral; D015723:Gene Library; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D033561:Penaeidae / Q000378:metabolism / Q000821:virology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D015398:Signal Transduction; D053676:Syntenins
D002352:Carrier Proteins; D018076:DNA, Complementary; D004279:DNA, Viral; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C109621:SDCBP protein, human; D053676:Syntenins; C033652:postsynaptic density proteins