Thin layer chromatographical detection of tyrosine produced from L-[U-(14)C]phenylalanine by ruminal microbes.
Thin layer chromatographical detection of tyrosine (Tyr) synthesized from L-[U-(14)C]phenylalanine (Phe) (1 mM) by rumen bacteria (B) and protozoa (P) collected from fistulated Japanese Goat was carried out. About 16 and 12% of the added Phe was converted to Tyr by B and P, respectively. Large amount of radioactivity in ether fractions indicated an abundant production of aromatic acids from Phe. Small amount of radioactivity found in CO(2) fractions implied an occurrence of considerable decarboxylation reaction(s) by rumen bacteria and protozoa.
Khan
R I
RI
Department of Animal Science, Faculty of Animal Husbandry, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. rikhanah@bdonline.com
Onodera
R
R
Amin
M R
MR
D000818:Animals; D001419:Bacteria / Q000378:metabolism; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D056890:Eukaryota / Q000378:metabolism; D006041:Goats; D010649:Phenylalanine / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology / Q000469:parasitology; D014443:Tyrosine / Q000032:analysis / Q000096:biosynthesis*
D002250:Carbon Radioisotopes; D014443:Tyrosine; D010649:Phenylalanine