Effect of artemether on hexokinase, glucose phosphate isomerase and phosphofructokinase of Schistosoma japonicum harbored in mice.
OBJECTIVE
To study the effect of artemether (Art) on hexokinase (HK), glucose phosphate isomerase (GPI) and phosphofructokinase (PFK) of Schistosoma japonicum.


METHODS
Mice infected with schistosome cercariae for 4-5 wk were treated ig with Art 100 or 300 mg.kg-1 and killed 24 h or 48 h after medication for collection of schistosomes. The activities of HK, GPI and PFK of the worms were determined by measuring the formation of NADPH or consumption of NADH.


RESULTS
Worms from the infected mice treated ig with Art at a single dose of 300 mg.kg-1 the inhibition rates of HK activity of female and male worms were 33.7% and 13.7%, respectively 24 h after administration. Similar results were seen in GPI activity, but 48 h after medication, the inhibition rate of GPI increased to 46.2% (female) and 32.9% (male), respectively. Worms from mice treated with Art 100 or 300 mg.kg-1, the inhibitory effect on PFK in female worms was found much higher than that of male worms the inhibition rates of PFK were 64.9%-71.0% in female worms and 16.3%-54.2% in male worms, respectively at 24 h and 48 h after treatment.


CONCLUSIONS
The results suggest that in the glycolytic pathway of schistosome PFK might be one of the targets attacked by Art.
Xiao
S
S
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, WHO Collaborating Center of Malaria, Schistosomiasis and Filariasis, Shanghai 200025.
You
J
J
Guo
H
H
Jiao
P
P
Mei
J
J
Yao
M
M
Feng
Z
Z
D000818:Animals; D000077549:Artemether; D037621:Artemisinins / Q000494:pharmacology*; D005260:Female; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D008297:Male; D051379:Mice; D025221:Phosphofructokinases / Q000378:metabolism*; D012549:Schistosoma japonicum / Q000201:enzymology*; D012556:Schistosomicides / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000494:pharmacology*
D037621:Artemisinins; D012556:Schistosomicides; D012717:Sesquiterpenes; D000077549:Artemether; D025221:Phosphofructokinases; D006593:Hexokinase; D005956:Glucose-6-Phosphate Isomerase