Segment 8 encodes a structural protein of infectious salmon anaemia virus (ISAV); the co-linear transcript from Segment 7 probably encodes a non-structural or minor structural protein.
In this study we present the cloning, expression and partial identification of Genomic Segment 7 of infectious salmon anaemia virus (ISAV). The nucleotide sequence corresponding to Segment 7 was isolated from a bacteriophage lambda cDNA library and contained 2 overlapping open reading frames (ORFs) of 903 and 522 bases respectively. It also contained an ISAV-specific conserved nucleotide motif in the mRNA 5' region. The co-linear transcript representing the large ORF undergoes a splicing event that removes a 526 nucleotide intron to form a mRNA corresponding to the smaller reading frame. Thus, ISAV Genomic Segment 7 has a similar coding strategy as influenza A virus Segments 7 and 8. The largest ORF of Segment 7 and the first ORF of Segment 8 was expressed in E. coli as fusion proteins and rabbit antiserum was raised against the recombinant protein from Segment 8. Immunoblot studies using this antiserum and a serum against purified virus, show that Segment 8 encodes one of the major structural proteins of the virus whereas the co-linear ORF of Segment 7 probably encodes a non- or minor structural protein
Bierin
Eirik
E
Intervet Norbio, Bergen, Norway. eirik.biering@intervet.com
Falk
Knut
K
Hoel
Eirik
E
Thevarajan
Jegatheswaran
J
Joerink
Maaike
M
Nylund
Are
A
Endresen
Curt
C
Krossøyl
Bjørn
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D004926:Escherichia coli; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009975:Orthomyxoviridae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011993:Recombinant Fusion Proteins / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D004279:DNA, Viral; D011993:Recombinant Fusion Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins