title: Dupuytren's disease: physiologic changes in nodule and cord fibroblasts through aging in vitro.
abstract: The pathogenesis of the fibrotic disease Dupuytren's contracture remains unclear. The disease process includes two structurally distinct fibrotic elements, the nodule and the cord. It has been proposed that as the disease progresses, nodules develop into cords. To corroborate that hypothesis, the authors took advantage of cultured fibroblast differences found between gap junction intercellular communication and fibroblast-populated collagen lattice contraction. Paired fibroblast cell lines of nodules and cords derived from four patients with Dupuytren's disease were maintained in culture for at least eight passages. The presence of gap junction intercellular communication in nodule- and cord-derived fibroblasts was documented and reported as a coupling index. The contraction of free-floating nodule- or cord-derived collagen lattices was also documented and reported. Early passage (passage 4) cord-derived fibroblasts showed a significant increase in coupling index compared with passage 4 nodule-derived fibroblasts (4.0 +/- 0.4 versus 2.5 +/- 0.3, respectively), where p < or = 0.01. However, late passage (passage 8) nodule- and cord-derived fibroblasts were equivalent in their coupling index (4.1 +/- 0.4 versus 4.4 +/- 0.4, respectively). Early passage nodule-derived fibroblast-populated collagen lattices contracted by 64 percent, whereas late passage nodule-derived lattices showed less contraction, at only 40 percent. Early and late passage cord-derived lattices contracted 46 and 37 percent, respectively. All nodule- and cord-derived cell lines were statistically equivalent at lattice contraction by passage 8. These in vitro studies support the hypothesis that fibroblasts derived from Dupuytren's contracture nodules change their phenotype after undergoing repeated cell passage, acquiring a cord-like fibroblast phenotype. Dupuytren's nodules represent the early, active form of fibrosis in which cells are more proliferative, better at fibroblast-populated collagen lattice contraction, and display less gap junction intercellular communication. The speculation is that alterations in gap junction intercellular communication may be involved in the progression of Dupuytren's nodules to cords as the disease progresses.
authors.affiliation: Division of Plastic Surgery, Milton S. Hershey Medical Center, 500 University Drive, Hershey, PA 17033, USA.
authors.forename: Kurtis E
authors.initials: KE
authors.lastname: Moyer
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Banducci
authors.forename: William P
authors.initials: WP
authors.lastname: Graham
authors.forename: H Paul
authors.initials: HP
authors.lastname: Ehrlich
mesh_terms: D002450:Cell Communication / Q000502:physiology; D002460:Cell Line; D016922:Cellular Senescence / Q000502:physiology*; D003094:Collagen / Q000502:physiology; D003599:Cytoskeleton / Q000473:pathology / Q000502:physiology; D004387:Dupuytren Contracture / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery; D005347:Fibroblasts / Q000473:pathology / Q000502:physiology*; D017629:Gap Junctions / Q000473:pathology / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques
chemical_list: D003094:Collagen