title: Microimmunoassay using a protein chip: optimizing conditions for protein immobilization.
abstract: Optimizing conditions for the microarraying of protein antigens onto glass slides were studied. Various vendors, surface functional groups, buffers, and fixatives were evaluated to enhance protein binding. A total of 125 pg of human immunoglobulin was detectable with this assay system, suggesting that protein microarray can be applied for routine immunodiagnosis.
authors.affiliation: Department of Microbiology and Immunology, Seoul National University College of Medicine and Institute of Endemic Disease, Seoul National University Medical Research Center, Chongno-gu, Seoul 110-799, Republic of Korea. sseong@niaid.nih.gov
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Seong
mesh_terms: D000941:Antigens / Q000276:immunology; D019921:Biotinylation; D005455:Fluorescent Antibody Technique; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000276:immunology; D007202:Indicators and Reagents; D011506:Proteins / Q000276:immunology; D011933:Reagent Kits, Diagnostic
chemical_list: D000941:Antigens; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D011506:Proteins; D011933:Reagent Kits, Diagnostic