Flow injection spectrophotometric determination of L-ascorbic acid in biological matters.
A method for spectrophotometric determination of L-ascorbic acid (vitamin C) in tissues by flow injection analysis is reported. The procedure is based on the oxidation of analyse with iron(III) and 2,2'-dipyridyl. Concentrations of vitamin C in the range of 0.5-20 ppm have been determined with a relative standard deviation 1.2% (n=15). The injection rate was 40 samples per h. The method was applied to the determination of L-ascorbic acid in rat's tissues (blood serum, brain, and liver) and compared favourably with an independent reference method based on spectrophotometry.
Kleszczewski
Tomasz
T
Institute of Biophysics, Medical Academy, Mickiewicza 2A, 15-230, Bialystok, Poland. kleszczt@cksr.ac.bialystok.pl
Kleszczewska
Ewa
E
D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis* / Q000097:blood; D001923:Brain Chemistry; D017022:Flow Injection Analysis; D008099:Liver / Q000737:chemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013053:Spectrophotometry
D001205:Ascorbic Acid