title: Laboratory bioassay studies to determine methoprene susceptibility in a natural population of Ochlerotatus taeniorhynchus from the Florida keys.
abstract: Larvae of Florida Keys Ochlerotatus taeniorhynchus collected from No Name Key were colonized and evaluated against technical S-methoprene in laboratory beaker tests. Glassware was treated with a silanizing reagent before testing to prevent methoprene attachment to the glassware. The No Name Key strain was compared with a susceptible laboratory strain of Oc. taeniorhynchus. Five serial dilutions (0.0100, 0.0050, 0.0010, 0.0005, and 0.0001 microg/ml) and an untreated control were evaluated. Tests were conducted in water baths with a constant water temperature of 27+/-1 degrees C and 250 ml of 3 per thousand salt water. Twenty-five late 3rd-stage larvae were placed in each beaker. Bioassay samples were analyzed by probit analysis and the median lethal concentration (LC50), 90% lethal concentration (LC90), and 95% lethal concentration (LC95) values; confidence limits; chi2 value; slope; and standard error were determined. The Florida Keys No Name Key strain exhibited no significant differences at the LC50, LC90, and LC95 levels from the laboratory strain in these studies.
authors.affiliation: John A. Mulrennan, Sr. Public Health Entomology Research and Education Center, Florida Agricultural and Mechanical University, Panama City 32405-1933, USA.
authors.forename: Omas G
authors.initials: OG
authors.lastname: Floore
authors.forename: John P
authors.initials: JP
authors.lastname: Smith
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Shaffer
authors.forename: Eric T
authors.initials: ET
authors.lastname: Schreiber
mesh_terms: D000818:Animals; D001681:Biological Assay; D009033:Culicidae; D005431:Florida; D007305:Insecticide Resistance; D007306:Insecticides; D007814:Larva; D008726:Methoprene; D009032:Mosquito Control
chemical_list: D007306:Insecticides; D008726:Methoprene