Tumor removal enhances immunity induced by B7.1.
Costimulation of tumor T cells by B7.1 has been shown to be important for eliciting cell-mediated anti-tumor immunity. We constructed a stable B7.1 gene transfectant of a poorly immunogenic murine sarcoma, Moloney murine sarcoma virus-induced tumor cell line (MMSV). This transfectant, MMSV-B7.1, failed to produce any tumor development in syngeneic mouse models. When MMSV-B7.1 was simultaneously injected with wild-type MMSV, about half of the coinjected mice remained tumor free and displayed an increase in T cell population, upregulation of the mRNA level of various cytokines such as IL-4, IL-5, IL-10, IL-13, IL-15 and IFN-gamma, and complete rejection of reinjected MMSV. To investigate whether MMSV-B7.1 demonstrates any vaccinal effect, the transfectant was injected following the surgical removal of the primary tumor mass. Following a re-challenge with wild-type MMSV, all vaccinated mice maintained their tumor free status and displayed a rapid recovery of down-regulated cytokine levels. The results suggest that B7.1 vaccination after tumor removal might be useful for the prevention of tumor recurrence.
Lee
M J
MJ
Department of Pathology and Cancer Research Institute, Seoul National University College of Medicine, Korea.
Choi
S H
SH
Shim
Y H
YH
Cho
S H
SH
Kim
C W
CW
D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002461:Cell Line, Transformed; D017690:Cell Transplantation; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D007167:Immunotherapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008980:Moloney murine sarcoma virus / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis; D012192:Retroviridae Infections / Q000276:immunology; D012513:Sarcoma, Experimental / Q000276:immunology* / Q000601:surgery / Q000628:therapy; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection; D014412:Tumor Virus Infections / Q000276:immunology
D018122:B7-1 Antigen; D016207:Cytokines; D012333:RNA, Messenger; D012334:RNA, Neoplasm