Cytotoxic constituents from Aquilegia ecalcarata.
A new alkaloid, 7-hydroxy-4-(5'-hydroxymethylfuran-2'-yl)-2-quinolone (1), and a new nitrile derivative, 3alpha,4beta-dihydroxy-6-oxo-1-cyclohexene-1-acetonitrile (2), together with three known oxoaporphine alkaloids, were isolated from the whole plant of Aquilegia ecalcarata. Their structures were established on the basis of spectral evidence. Their in vitro cytotoxicity against different classes of cancer cell lines, including GLC-82 and HCT were determined. The new alkaloid 1 [IC 50 (GLC-82) 8.8 +/- 0.2 microM, IC 50 (HCT) 10.1 +/- 0.3 microM] and hernandonine ( 3) [IC 50 (GLC-82) 7.6 +/- 0.5 microM, IC 50 (HCT) 8.2 +/- 0.5 microM] exhibited cytotoxicity towards the cancer cell lines.
Chen
Si-Bao
SB
Gao
Guang-Yao
GY
Li
Ye-Shi
YS
Yu
Shi-Chun
SC
Xiao
Pei Gen
PG
D000097:Acetonitriles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D053138:Cyclohexenes; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology; D015363:Quinolones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D029626:Ranunculaceae; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects
C465100:3,4-dihydroxy-6-oxo-1-cyclohexene-1-acetonitrile; C465099:7-hydroxy-4-(5'-hydroxymethylfuran-2'-yl)-2-quinolone; D000097:Acetonitriles; D000470:Alkaloids; D053138:Cyclohexenes; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D015363:Quinolones; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue