title: Monoclonal B lymphocytes with the characteristics of "indolent" chronic lymphocytic leukemia are present in 3.5% of adults with normal blood counts.
abstract: Molecular and cellular markers associated with malignant disease are frequently identified in healthy individuals. The relationship between these markers and clinical disease is not clear, except where a neoplastic cell population can be identified as in myeloma/monoclonal gammopathies of undetermined significance (MGUS). We have used the distinctive phenotype of chronic lymphocytic leukemia (CLL) cells to determine whether low levels of these cells can be identified in individuals with normal complete blood counts. CLL cells were identified by 4-color flow cytometric analysis of CD19/CD5/CD79b/CD20 expression in 910 outpatients over 40 years old. These outpatients were age- and sex-matched to the general population with normal hematologic parameters and no evident history of malignant disease. CLL phenotype cells were detectable in 3.5% of individuals at low level (median, 0.013; range, 0.002- 1.458 x 10(9) cells/L), and represented a minority of B lymphocytes (median, 11%; range, 3%-95%). Monoclonality was demonstrated by immunoglobulin light-chain restriction in all cases with CLL phenotype cells present and confirmed in a subset of cases by consensus-primer IgH-polymerase chain reaction. As in clinical disease, CLL phenotype cells were detected with a higher frequency in men (male-to-female ratio, 1.9:1) and elderly individuals (2.1% of 40- to 59-year-olds versus 5.0% of 60- to 89-year-olds, P =.01). The neoplastic cells were identical to good-prognosis CLL, being CD5+23+20(wk)79b(wk)11a(-)22(wk)sIg(wk)CD38-, and where assessed had a high degree (4.8%-6.6%) of IgH somatic hypermutation. The monoclonal CLL phenotype cells present in otherwise healthy individuals may represent a very early stage of indolent CLL and should be useful in elucidating the mechanisms of leukemogenesis.
authors.affiliation: Haematological Malignancy Diagnostic Service, Academic Unit of Haematology and Oncology, Algernon Firth Building, University of Leeds, United Kingdom. andy.rawstron@hmds.org.uk
authors.forename: Andy C
authors.initials: AC
authors.lastname: Rawstron
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Green
authors.forename: Anita
authors.initials: A
authors.lastname: Kuzmicki
authors.forename: Ben
authors.initials: B
authors.lastname: Kennedy
authors.forename: James A L
authors.initials: JA
authors.lastname: Fenton
authors.forename: Paul A S
authors.initials: PA
authors.lastname: Evans
authors.forename: Sheila J M
authors.initials: SJ
authors.lastname: O'Connor
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Richards
authors.forename: Gareth J
authors.initials: GJ
authors.lastname: Morgan
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Jack
authors.forename: Peter
authors.initials: P
authors.lastname: Hillmen
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000473:pathology*; D001772:Blood Cell Count; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D002999:Clone Cells / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D005803:Genes, Immunoglobulin; D006801:Humans; D016130:Immunophenotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000453:epidemiology* / Q000473:pathology; D008297:Male; D008403:Mass Screening / Q000379:methods; D008875:Middle Aged; D015995:Prevalence; D012737:Sex Factors