Redox status-dependent regulation of cyclooxygenases mediates the capsaicin-induced apoptosis in human neuroblastoma cells.
Cyclooxygenases (COX) appear to be involved in the mechanism of apoptosis in various cancer cells. In this study we investigated the role of COX in the capsaicin (Cap)-induced apoptosis in SK-N-SH human neuroblastoma cells. Cap induced decreased cell viability and apoptosis in a dose-dependent manner. Cap also significantly reduced the basal generation of reactive oxygen species (ROS) and lipid peroxidation in a time-dependent fashion. Cap markedly suppressed the expression of COX-1 and COX-2. Pretreatment with NS-398, a selective COX-2 inhibitor, or indomethacin, a nonselective COX inhibitor, significantly enhanced the Cap-induced decreased cell viability and apoptosis. Exogenous application of an oxidant, H2O2, significantly prevented the Cap-induced apoptosis and suppressed the expression of COX isoforms. These results suggest that redox status-dependent regulation of COX expression may mediate apoptosis induced by Cap in human neuroblastoma cells.
Lee
Yong Soo
YS
Department of Physiology, College of Medicine, Kwandong University, Kangnung, Korea.
Kwon
Eun Jin
EJ
Jin
Da Qing
DQ
Park
Seung Hee
SH
Kang
Young Shin
YS
Huh
Keun
K
Kim
Jung-Ae
JA
D017209:Apoptosis / Q000502:physiology*; D002211:Capsaicin / Q000494:pharmacology*; D002470:Cell Survival; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis* / Q000494:pharmacology; D008565:Membrane Proteins; D009447:Neuroblastoma / Q000473:pathology*; D016877:Oxidants; D010084:Oxidation-Reduction; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; D008565:Membrane Proteins; D016877:Oxidants; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D002211:Capsaicin