title: Two DBLgamma subtypes are commonly expressed by placental isolates of Plasmodium falciparum.
abstract: Adhesion to chondroitin sulfate A (CSA), a distinguishing feature of malaria parasites obtained from the human placenta, might be mediated by the Duffy-binding-like (DBL) gamma domain of the variant surface antigen Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1). We studied transcription of var genes (that encode PfEMP1) in placental parasites by amplifying and sequencing DBLgamma fragments from genomic DNA and cDNA of field isolates collected in western Kenya. We amplified DBLgamma fragments with divergent sequences from individual isolates by using various sequence-specific or degenerate primers. Transcripts detected with degenerate primers clustered phylogenetically within two DBLgamma subtypes with homology to chr5_1.gen_150 or FCR3.varCSA. Interestingly, the DBLalpha encoded by chr5_1.gen_150 was recently found to be commonly expressed by placental isolates from Malawi (Mol. Biochem. Parasitol. 185 (2002) 1207). The findings are consistent with earlier serologic evidence that surface antigens of placental parasites have conserved features, and suggest that vaccines based on DBLgamma may only need to target a limited number of variants.
authors.affiliation: Seattle Biomedical Research Institute, 4 Nickerson Street, WA 98109, USA. mfried@sbri.org
authors.forename: Michal
authors.initials: M
authors.lastname: Fried
authors.forename: Patrick E
authors.initials: PE
authors.lastname: Duffy
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D017931:DNA Primers; D004375:Duffy Blood-Group System / Q000145:classification / Q000378:metabolism*; D004912:Erythrocytes / Q000469:parasitology*; D005260:Female; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology; D008875:Middle Aged; D008969:Molecular Sequence Data; D010920:Placenta / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000378:metabolism / Q000472:pathogenicity*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000469:parasitology*; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D004375:Duffy Blood-Group System; D015800:Protozoan Proteins; C085731:erythrocyte membrane protein 1, Plasmodium falciparum