title: Developmental changes in KCC1, KCC2 and NKCC1 mRNAs in the rat cerebellum.
abstract: Cation chloride cotransporters are considered to play pivotal roles in controlling the intracellular and extracellular ionic environments of neurons, hence controlling neuronal function. To establish how these cotransporters are involved in cerebellum development, we investigated the expression of KCC1, KCC2 and NKCC1 mRNAs in the developing rat cerebellum using in situ hybridization histochemistry. In the external germinal layer, where premature cells exist, we found substantial KCC1 and NKCC1 mRNA expression on P7 and P14, while KCC2 mRNA was not detected. In contrast, KCC2 mRNA was already expressed in Purkinje cells on P1. We also observed KCC2 mRNA expression in postmigratory granule cells after P7. The expression of KCC1, KCC2, and NKCC1 mRNAs reached adult patterns by P21. In the adult cerebellum, KCC2 mRNA was expressed in most neurons, including Purkinje cells, granule cells, and stella/basket cells, while KCC1 and NKCC1 mRNAs were only detected in granule cells and glial cells. These findings suggest that in the rat cerebellum KCC2 mRNA expression is induced when neurons arrive their final destinations.
authors.affiliation: Department of Anatomy and Neuroscience, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, Shizuoka 431-3192, Japan.
authors.forename: Sumiko
authors.initials: S
authors.lastname: Mikawa
authors.forename: Cong
authors.initials: C
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Shu
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Atsuo
authors.initials: A
authors.lastname: Fukuda
authors.forename: Kohji
authors.initials: K
authors.lastname: Sato
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000235:genetics; D002462:Cell Membrane / Q000378:metabolism*; D002529:Cerebellar Nuclei / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D018118:Chloride Channels / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D007424:Intracellular Fluid / Q000378:metabolism; D008297:Male; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D028021:Sodium-Potassium-Chloride Symporters / Q000235:genetics*; D064506:Solute Carrier Family 12, Member 2; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D027981:Symporters / Q000235:genetics*; D000096922:K Cl- Cotransporters
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D012333:RNA, Messenger; D028021:Sodium-Potassium-Chloride Symporters; D064506:Solute Carrier Family 12, Member 2; D027981:Symporters; D000096922:K Cl- Cotransporters; C578879:Slc12a2 protein, rat