title: Band-3 protein function in human erythrocytes: effect of oxygenation-deoxygenation.
abstract: Sulfate transport by band-3 protein in adult human erythrocytes was shown to be modulated by oxygen pressure. In particular, a higher transport activity was measured under high oxygen pressure than at low one (0.0242+/-0.0073 vs. 0.0074+/-0.0010 min(-1)). Other factors, such as magnesium ions and orthovanadate, which can indirectly affect the binding properties of the cytoplasmic domain of band 3 (cdb3), influence significantly the anion exchanger activity. No effect of oxygen pressure on sulfate transport was found in chicken erythrocytes, which may be related to their lacking the cdb3 binding site. These findings are fully consistent with a molecular mechanism where the oxygen-linked transition of hemoglobin (T-->R) could play a key role in the regulation of anion exchanger activity.
authors.affiliation: Dipartimento di Chimica Organica e Biologica, Università di Messina, Messina, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Galtieri
authors.forename: Ester
authors.initials: E
authors.lastname: Tellone
authors.forename: Leonardo
authors.initials: L
authors.lastname: Romano
authors.forename: Francesco
authors.initials: F
authors.lastname: Misiti
authors.forename: Ersilia
authors.initials: E
authors.lastname: Bellocco
authors.forename: Silvana
authors.initials: S
authors.lastname: Ficarra
authors.forename: Annamaria
authors.initials: A
authors.lastname: Russo
authors.forename: Domenica
authors.initials: D
authors.lastname: Di Rosa
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnola
authors.forename: Bruno
authors.initials: B
authors.lastname: Giardina
authors.forename: Irene
authors.initials: I
authors.lastname: Messana
mesh_terms: D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry* / Q000378:metabolism*; D002645:Chickens; D004912:Erythrocytes / Q000378:metabolism*; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D017136:Ion Transport; D007700:Kinetics; D010100:Oxygen / Q000097:blood; D011312:Pressure; D013045:Species Specificity; D013431:Sulfates / Q000097:blood
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D006454:Hemoglobins; D013431:Sulfates; D010100:Oxygen