Purification and biological characterization of halocin H1 from Haloferax mediterranei M2a.
The production of halocins, bacteriocin-like proteins of ecological significance, is a frequent characteristic of species from the family Halobacteriaceae. Halocin H1, produced by Haloferax mediterranei strain M2a, is a single 31-kDa polypeptide. Its purification was achieved by combining two chromatographic systems: Sepharose 4B linked to bacitracin followed by hydroxylapatite Bio-gel HTP. Halocin H1 required concentrations of NaCl higher than 1.5 M to maintain its activity. Haoarchaeal strains showed a differential degree of sensitivity to the action of this halocin.
Platas
Gonzalo
G
Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Cantoblanco, Spain. gonzalo_platas@merck.com
Meseguer
Inmaculada
I
Amils
Ricardo
R
D001430:Bacteriocins / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology*; D017753:Ecosystem; D019623:Haloferax mediterranei / Q000378:metabolism; D009994:Osmolar Concentration; D012492:Salts / Q000494:pharmacology; D012965:Sodium Chloride / Q000737:chemistry
D001430:Bacteriocins; D012492:Salts; D012965:Sodium Chloride