title: RNA 2 of Citrus psorosis virus is of negative polarity and has a single open reading frame in its complementary strand.
abstract: Citrus psorosis virus (CPsV) causes a citrus disease occurring worldwide. Isolate CPV 4 has a genome with three single-stranded RNAs. The complete sequence of RNA 2 (1643 nucleotides) is reported here. Northern blot hybridization with strand-specific probes showed that most of the encapsidated RNA 2 is of negative polarity, although a small amount of the complementary strand may also be present in particles. The RNA 2 complementary strand contained a single open reading frame encoding a protein of 476 amino acids, which includes a motif resembling a nuclear localization signal. The sequence of this putative protein shows no significant similarity to any other in the databases. In the 3'-terminal untranslated region there is a putative polyadenylation signal. No subgenomic RNAs derived from RNA 2 were detected.
authors.affiliation: Instituto de Bioquímica y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115-1900 La Plata, Argentina1.
authors.forename: María Eugenia Sánchez
authors.initials: MES
authors.lastname: de la Torre
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, Spain2.
authors.forename: Carmelo
authors.initials: C
authors.lastname: López
authors.affiliation: Instituto de Bioquímica y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115-1900 La Plata, Argentina1.
authors.forename: Oscar
authors.initials: O
authors.lastname: Grau
authors.affiliation: Instituto de Bioquímica y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115-1900 La Plata, Argentina1.
authors.forename: María Laura
authors.initials: ML
authors.lastname: García
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002957:Citrus / Q000821:virology*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019913:Nuclear Localization Signals; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000737:chemistry / Q000235:genetics*; D012328:RNA Viruses / Q000737:chemistry / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D019913:Nuclear Localization Signals; D012367:RNA, Viral; D014764:Viral Proteins