title: Regulation of insulin-like growth factor binding protein secretion by human granulosa luteal cells in a polycystic ovary-like environment.
abstract: OBJECTIVE
  To determine the effect of androstenedione (A), insulin, and LH on secretion of insulin-like growth factor binding proteins (IGFBPs) from human granulosa luteal cells.
  
  
  METHODS
  Human granulosa cells were cultured for a total of 4 days in serum-free medium containing A (10(-6) mol/L), with or without insulin (100 microU/mL-800 microU/mL), LH (1 microU/mL-10 microg/L), and A (10(-5) mol/L).
  
  
  METHODS
  Granulosa cells were obtained from IVF procedures.
  
  
  METHODS
  Women undergoing IVF for tubal disease.
  
  
  METHODS
  None.
  
  
  METHODS
  Immunoassay and autoradiographs of Western ligand blotting detected IGFBP accumulations in the medium.
  
  
  RESULTS
  Cultured granulosa cells secreted IGFBP-1 through IGFBP-4. Insulin (100 microU/mL-800 microU/mL), LH (1 microg/L-10 microg/L), and A (10(-5) mol/L) caused a significant decrease in IGFBP-1 accumulation in the medium both alone and when added in combination. The release of IGFBP-2 and IGFBP-4 was significantly stimulated by insulin, whereas LH had no effect. Elevated levels of androgen (10(-5) mol/L) significantly stimulated the secretion of IGFBP-2, whereas the release of IGFBP-4 was reduced.
  
  
  CONCLUSIONS
  These results demonstrate that androgen and insulin are important regulators of IGFBP release and that elevated levels of the two hormones may contribute to the altered IGFBP profile found in PCOS follicles, compared with the case of estrogen-dominant follicles.
authors.affiliation: Department of Gynecology and Obstetrics, Aarhus University Hospital, Aarhus, Denmark. greisens@mail1.stofanet.dk
authors.forename: Susanne
authors.initials: S
authors.lastname: Greisen
authors.forename: Allan
authors.initials: A
authors.lastname: Flyvbjerg
authors.forename: Thomas
authors.initials: T
authors.lastname: Ledet
authors.forename: Per
authors.initials: P
authors.lastname: Ovesen
mesh_terms: D000735:Androstenedione / Q000008:administration & dosage / Q000494:pharmacology; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology; D018969:Insulin-Like Growth Factor Binding Proteins / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000008:administration & dosage / Q000494:pharmacology; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism
chemical_list: D007328:Insulin; D018969:Insulin-Like Growth Factor Binding Proteins; D020033:Protein Isoforms; D000735:Androstenedione; D007986:Luteinizing Hormone