Different osteochondral potential of clonal cell lines derived from adult human trabecular bone.
Cells derived from human trabecular bones have been shown to have multipotential differentiation ability along osteogenic, chondrogenic, and adipogenic lineages. In this study, we have derived two clonal sublines of human trabecular bone cells by means of stable transduction with human papilloma virus E6/E7 genes. Our results showed that these clonal sublines differ in their osteochondral potential, but are equally adipogenic, indicative of the heterogeneous nature of the parental cell population. The availability of these cell lines should be useful for the analysis of the mechanisms regulating the differentiation of adult mesenchymal progenitor cells.
Osyczka
Anna M
AM
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Nöth
Ulrich
U
Danielson
Keith G
KG
Tuan
Rocky S
RS
D000328:Adult; D001842:Bone and Bones / Q000166:cytology* / Q000502:physiology; D002460:Cell Line; D019070:Cell Lineage; D002478:Cells, Cultured; D005822:Genetic Vectors; D006801:Humans; D007150:Immunohistochemistry; D027383:Papillomaviridae / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology; D014161:Transduction, Genetic