title: Ceftriaxone induced immune hemolytic anemia: detection of drug-dependent antibody by ex-vivo antigen in urine.
abstract: There have been a few reported cases of immune hemolytic anemia induced by ceftriaxone. We encountered a patient with immune hemolytic anemia that seemed to be stimulated by a degradation product of ceftriaxone. The patient's direct antiglobulin test was positive only for C3d, and no ceftriaxone-dependent antibodies were detectable in the patient's serum. To demonstrate the presence of the ceftriaxone-induced antibodies, an ex-vivo antigen in urine was obtained from the patient. In addition, we prepared a 1 mg/mL suspension solution of ceftriaxone, and group AB serum as a complement source. Using several combinations of the above reactants, the indirect antiglobulin test was performed. Only the indirect antiglobulin test using the patient's serum with the ex-vivo urine antigen was found to be positive. Other combinations were not reactive. To our knowledge, this is the first reported case in Korea, in which the causative antibody appeared to be stimulated solely by a degradation product of ceftriaxone.
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine, C.P.O. Box 8044, Seoul 120-752, Korea.
authors.forename: Sinyoung
authors.initials: S
authors.lastname: Kim
authors.forename: Kyung Soon
authors.initials: KS
authors.lastname: Song
authors.forename: Hyun Ok
authors.initials: HO
authors.lastname: Kim
authors.forename: Hwan Mo
authors.initials: HM
authors.lastname: Lee
mesh_terms: D000744:Anemia, Hemolytic, Autoimmune / Q000139:chemically induced* / Q000175:diagnosis* / Q000276:immunology / Q000652:urine; D000941:Antigens / Q000652:urine; D002443:Ceftriaxone / Q000009:adverse effects*; D002511:Cephalosporins / Q000009:adverse effects*; D003298:Coombs Test; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D000941:Antigens; D002511:Cephalosporins; D002443:Ceftriaxone