title: The membrane-associated F(0)F(1) ATPase is essential for the viability of Streptococcus pneumoniae.
abstract: Genetic studies aimed at eliminating expression of the atp operon (F(0)F(1) H(+)-ATPase) of Streptococcus pneumoniae by genetic disruption of atpC, the first gene of the operon, with a chloramphenicol-resistance cassette were performed. Resistant transformants were obtained only when the recipient strain had a duplication of atpC, recombination occurring in such a way that transcription of the operon from its own promoter was allowed. These results imply that the atp operon is essential for the viability of the cells.
authors.affiliation: Unidad de Genética Bacteriana (Consejo Superior de Investigaciones Científicas), Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain
authors.forename: María José
authors.initials: MJ
authors.lastname: Ferrándiz
authors.forename: Adela G
authors.initials: AG
authors.lastname: de la Campa
mesh_terms: D002462:Cell Membrane / Q000201:enzymology*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D020043:Genes, Essential; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000254:growth & development*; D014169:Transformation, Bacterial
chemical_list: D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase; C103607:mitochondrial ATPase subunit c; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases