title: Jasplakinolide reversibly disrupts actin filaments in suspension-cultured tobacco BY-2 cells.
abstract: Jasplakinolide is potentially a useful pharmacological tool for the study of actin organization and dynamics in living cells, since it induces actin polymerization in vitro and, unlike phalloidin, is membrane permeative. In the present work, the effect of jasplakinolide on the actin cytoskeleton of living suspension-cultured Nicotiana tabacum 'Bright Yellow 2' cells was investigated. Actin filaments in the living cells were disrupted by jasplakinolide. The effect of jasplakionlide on the actin cytoskeleton was concentration and time dependent. When cells were treated with a moderate concentration (150 nM) of jasplakinolide, cortical actin filaments were disrupted preferentially, whereas actin aggregated at the perinuclear region. With concentrations higher than 400 nM and exposure times longer than 30 min, actin filaments in the cell disappeared completely. The effect of jasplakinolide on the actin cytoskeleton was reversible even at high concentration. Actin bundles appeared first in the perinuclear region within 5 min, and the cortical actin array was reestablished in 15 min, suggesting that actin filaments might be organized at this region.
authors.affiliation: Key Laboratory of Plant Physiology and Biochemistry of Ministry of Education, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing.
authors.forename: G S
authors.initials: GS
authors.lastname: Ou
authors.forename: Z L
authors.initials: ZL
authors.lastname: Chen
authors.forename: M
authors.initials: M
authors.lastname: Yuan
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D047630:Depsipeptides; D009994:Osmolar Concentration; D010456:Peptides, Cyclic / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors; D014026:Nicotiana / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure*
chemical_list: D047630:Depsipeptides; D010456:Peptides, Cyclic; C057531:jasplakinolide