title: Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein.
abstract: Smad4 is a novel tumor suppressor gene which is mutated or deleted in about 50% of pancreatic carcinoma and 30% of colon cancer. SMAD4 was expressed in Pichia pastoris and was characterized. The molecular weight of the expression product was shown to be about 67 kD, and 13 amino acids in N terminal were determined and were identical with the putative sequence from Smad4 cDNA, and it was able to specifically react with the antibody against SMAD4.
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. huangc@nic.bmi.ac.cn
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Liu
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Xu
authors.forename: Ji-Xian
authors.initials: JX
authors.lastname: Deng
authors.forename: Xuan
authors.initials: X
authors.lastname: Cheng
authors.forename: Cui-Fen
authors.initials: CF
authors.lastname: Huang