title: Preparation of samples for gas chromatography/mass spectrometry analysis of phthalate and adipate esters in plasma and beverages by steam distillation and extraction.
abstract: Phthalate and adipate esters are present in relatively large amounts in the environment, resulting in their large blank values at analysis and making precise analysis difficult. We developed a highly sensitive analytical method for phthalate and adipate esters in plasma and beverages by lowering the blank values that interfere with analysis. The method uses a closed distillation cleanup system in which steam distillation and extraction are performed simultaneously. The recoveries from beverages and plasma were both satisfactory, ranging from 90.2 to 118.3%, relative standard deviation (RSD) = 2.8-5.3%, and 96.2-134.4%, RSD = 2.2-6.5%, respectively. The detection limits of dibutyl phthalate and di-2-ethyl hexyl phthalate were 5 ng/mL, and those of diethyl phthalate, butyl benzyl phthalate, and di-2-ethyl hexyl adipate were 10 ng/mL in rabbit plasma and beverages.
authors.affiliation: Hoshi University, Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Tokyo, Japan.
authors.forename: Kayoko
authors.initials: K
authors.lastname: Kato
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazawa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ito
authors.forename: Hisao
authors.initials: H
authors.lastname: Oka
mesh_terms: D000272:Adipates / Q000032:analysis* / Q000097:blood; D000818:Animals; D001628:Beverages / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D010795:Phthalic Acids / Q000032:analysis* / Q000097:blood; D010968:Plasticizers / Q000032:analysis; D011817:Rabbits
chemical_list: D000272:Adipates; D010795:Phthalic Acids; D010968:Plasticizers