Implication of p53 and caspase-3 in kainic acid but not in N-methyl-D-aspartic acid-induced apoptosis in organotypic hippocampal mouse cultures.
Apoptotic death is known to be an active process requiring the activation of several apoptotic proteins. Depending on the tissue studied and the stimulus used, these processes are distinct. In this work, we studied if there is a putative implication of the p53 and the caspase-3 proteins in kainic acid (KA) and N-methyl-D-aspartic acid (NMDA)-induced apoptosis in organotypic cultures and if there is any relationship between their respective expressions. We found that KA and NMDA both induce apoptosis but only KA-induced apoptosis is p53- and caspase-3-dependent. This demonstrates that KA and NMDA induce apoptosis following different intracellular pathways.
Djebaïli
Myriam
M
UPR CNRS 9023, CCIPE, Laboratoire de Médecine Expérimentale, Institut de Biologie, Boulevard Henri IV, 34094 Montpellier Cedex 5, France. mdjebai@emory.edu
De Bock
Frédéric
F
Baille
Valérie
V
Bockaert
Joël
J
Rondouin
Gérard
G
D000818:Animals; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D053938:DNA Fragmentation / Q000187:drug effects / Q000502:physiology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology*; D007608:Kainic Acid / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000166:cytology* / Q000201:enzymology; D009498:Neurotoxins / Q000494:pharmacology; D009924:Organ Culture Techniques; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D018690:Excitatory Amino Acid Agonists; D009498:Neurotoxins; D016159:Tumor Suppressor Protein p53; D016202:N-Methylaspartate; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases; D007608:Kainic Acid