title: [Influence of a bolus injection of thiopental and propofol on the expression density of P-selectin and fibrinogen receptors on the surface of human platelets ex vivo].
abstract: OBJECTIVE
  Exposure of platelets to anaesthetic agents may alter their functional characteristics and in patients undergoing surgery or long-term sedation this may be clinically relevant. We investigated the influence of thiopentone and propofol on the expression of platelet membrane P-selectin, fibrinogen receptor, and leukocyte-platelet aggregates ex vivo.
  
  
  METHODS
  In patients undergoing surgery blood samples were taken from a peripheral vein cannula before induction of anaesthesia with propofol (3.0 mg kg (-1) BW) or thiopentone (7.0 mg kg (-1) BW) and five minutes later (n = 11 in each group). Surface expression of the fibrinogen receptor complex (GP Ib/IIIa), P-selectin, and the percentage of leukocyte-platelet aggregates were measured by flow cytometry after stimulation via the ADP or thrombin receptor pathway.
  
  
  RESULTS
  There was no significant difference in the expression of gpIIb/IIIa, P-selectin, and percentage of leukocyte-platelet aggregates in the thiopentone and propofol group after induction of anaesthesia.
  
  
  CONCLUSIONS
  In clinically relevant concentrations thiopentone and propofol did not influence the surface expression density of fibrinogen receptors, P-selectin molecules, and the percentage of leukocyte-platelet aggregates ex vivo.
authors.affiliation: Zentrum Anästhesiologie, Medizinische Hochschule Hannover. scheinichen.dirk@mh-hannover.de
authors.forename: D
authors.initials: D
authors.lastname: Scheinichen
authors.forename: A
authors.initials: A
authors.lastname: Bornscheuer
authors.forename: K
authors.initials: K
authors.lastname: Jaeger
authors.forename: K-H
authors.initials: KH
authors.lastname: Mahr
authors.forename: M
authors.initials: M
authors.lastname: Koksch
authors.forename: H
authors.initials: H
authors.lastname: Ruschulte
authors.forename: J
authors.initials: J
authors.lastname: Heine
mesh_terms: D018686:Anesthetics, Intravenous / Q000008:administration & dosage / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000096:biosynthesis*; D010974:Platelet Aggregation / Q000187:drug effects; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000096:biosynthesis* / Q000187:drug effects / Q000378:metabolism; D015742:Propofol / Q000008:administration & dosage / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000187:drug effects*; D013874:Thiopental / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D018686:Anesthetics, Intravenous; D019007:P-Selectin; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D011956:Receptors, Cell Surface; D013874:Thiopental; D015742:Propofol