Cloning of a gene encoding raw-starch-digesting amylase from a Cytophaga sp. and its expression in Escherichia coli.
A raw-starch-digesting amylase (RSDA) gene from a Cytophaga sp. was cloned and sequenced. The predicted protein product contained 519 amino acids and had high amino acid identity to alpha-amylases from three Bacillus species. Only one of the Bacillus alpha-amylases has raw-starch-digesting capability, however. The RSDA, expressed in Escherichia coli, had properties similar to those of the enzyme purified from the Cytophaga sp.
Jeang
Chii-Ling
CL
Department of Food Science, National Chung Hsing University, 250 Kuokang Road, Taichung 402, Taiwan, Republic of China. cljeang@nchu.edu.tw
Chen
Li-Shien
LS
Chen
Ming-Yu
MY
Shiau
Rong-Jen
RJ
D003001:Cloning, Molecular; D003589:Cytophaga / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis; D000516:alpha-Amylases / Q000235:genetics* / Q000276:immunology
D004269:DNA, Bacterial; D011994:Recombinant Proteins; D000516:alpha-Amylases