Effects of gamma irradiation on the development of Trypanosoma rangeli in the vector Rhodnius prolixus.
Studies on the effects of gamma radiation on the infectivity of Trypanosoma rangeli (strain H14) for the vector Rhodnius prolixus revealed that (i) the LD(50) (lethal dose for 50% of bugs) for uninfected insects was 4147 rads; (ii) irradiated insects with a dose of 1200 rads subsequently infected with the flagellates exhibited a mortality of 45%, while uninfected irradiated insects showed a mortality of 5%, and infected nonirradiated insects exhibited 10% mortality; (iii) flagellates were present in the hemolymph of irradiated insects 7 days postinfection (p.i.), while in nonirradiated insects the parasites appeared in the hemocoel 18 days p.i.; (iv) T. rangeli infection decreased the number of hemocytes significantly and induced the formation of nodules in the hemolymph of both irradiated and nonirradiated insects; and (v) gamma irradiation affected the ultrastructural organization of the epithelial cells of the small intestine, principally the perimicrovillar membranes and microvilli. In this paper, we discuss the significance of the intestinal microenvironment of R. prolixus with regard to its interaction with T. rangeli.
Gomes
S A O
SA
Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, Fiocruz, Av. Brazil 4365, Manguinhos, CEP 21045-900, Rio de Janeiro, RJ, Brazil.
Graciano
G L
GL
Nogueira
N F S
NF
De Souza
W
W
Garcia
E S
ES
Azambuja
P
P
D000818:Animals; D005720:Gamma Rays; D007303:Insect Vectors; D012239:Rhodnius / Q000469:parasitology*; D014345:Trypanosoma / Q000472:pathogenicity* / Q000528:radiation effects*