title: Optimisation of the degenerate oligonucleotide primed PCR (DOP-PCR) for capillary thermocycler.
abstract: DOP-PCR was optimised for capillary thermocycler. Five primers with five or six nucleotide anchor sequences and different restriction enzyme recognition sites were tested. The efficiency of the amplification was confirmed by gel electrophoresis and fluorescence in situ hybridisation on metaphase chromosomes.
authors.affiliation: Microbiology and Tumor Biology Center (MTC), Karolinska Institute, Nobels vag 16, S-17177 Stockholm, Sweden. csaba.kiss@mtc.ki.se
authors.forename: Csaba
authors.initials: C
authors.lastname: Kiss
authors.forename: Maria
authors.initials: M
authors.lastname: Kost-Alimova
authors.forename: George
authors.initials: G
authors.lastname: Klein
authors.forename: Laszlo
authors.initials: L
authors.lastname: Szekely
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D011786:Quality Control; D017384:Sequence Deletion; D019914:Taq Polymerase / Q000235:genetics*; D013696:Temperature
chemical_list: D017931:DNA Primers; D019914:Taq Polymerase