title: Identification of autoantibodies associated with systemic lupus erythematosus.
abstract: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the presence of antinuclear antibodies. We performed serological analysis of cDNA expression library (SEREX) to identify autoantibodies associated with SLE. The screening of three different cDNA expression libraries with pooled sera of patients with SLE yielded 11 independent clones that reacted with pooled sera of patients with SLE. In this screening, autoantibodies to poly(ADP-ribose) polymerase (PARP), U1snRNP, and galectin-3 were prevalent in the sera of patients with SLE (26/68, 25/68, 12/63, respectively). The frequency of autoantibody to PARP was significantly higher in SLE than that of healthy donors (0/76) (38.2% vs 0%, p<0.00001). The autoantibody to PARP was infrequently detected in the serum of patients with RA (1/50). However, autoantibody to PARP was not found in the sera of patients with other rheumatic diseases including Sjogren's syndrome (0/19), systemic sclerosis (0/18), and polymyositis/myositis (0/37). The frequency of autoantibody to human galectin-3 (12/63) was significantly higher in SLE than that of healthy donors (0/56) (19% vs 0%, p=0.0006). Autoantibody to galectin-3 was not found in the sera of patients with rheumatoid arthritis (0/50), Sjogren's syndrome (0/18), and systemic sclerosis (0/19). Interestingly, autoantibody to galectin-3 was also prevalent in the sera of patients with polymyositis/dermatomyositis (16/37, 43.2%). Further functional characterization of these autoantibodies would be necessary to determine their value as diagnostic markers or to define clinical subsets of patients with SLE. Statistical analysis revealed that the presence of autoantibody to PARP was inversely related with pleurisy, and the presence of autoantibody to galectin-3 related with renal disease.
authors.affiliation: Cancer Genomics Division, In2Gen Company, 6th Floor, Cancer Research Center, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea.
authors.forename: Yoon
authors.initials: Y
authors.lastname: Lim
authors.forename: Dae-Yeon
authors.initials: DY
authors.lastname: Lee
authors.forename: Seongeun
authors.initials: S
authors.lastname: Lee
authors.forename: Sae-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Jongwan
authors.initials: J
authors.lastname: Kim
authors.forename: Bomsoo
authors.initials: B
authors.lastname: Cho
authors.forename: Hosoon
authors.initials: H
authors.lastname: Lee
authors.forename: Hae-Yeong
authors.initials: HY
authors.lastname: Kim
authors.forename: Eunbong
authors.initials: E
authors.lastname: Lee
authors.forename: Yeong Wook
authors.initials: YW
authors.lastname: Song
authors.forename: Doo-Il
authors.initials: DI
authors.lastname: Jeoung
mesh_terms: D000328:Adult; D000943:Antigens, Differentiation / Q000276:immunology; D001323:Autoantibodies / Q000097:blood / Q000235:genetics / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology; D005260:Female; D037502:Galectin 3; D015723:Gene Library; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000276:immunology*; D008297:Male; D011065:Poly(ADP-ribose) Polymerases / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000276:immunology
chemical_list: D000943:Antigens, Differentiation; D001323:Autoantibodies; D001324:Autoantigens; D037502:Galectin 3; D012333:RNA, Messenger; D017412:Ribonucleoprotein, U1 Small Nuclear; D011065:Poly(ADP-ribose) Polymerases