Apoptosis of human highly metastatic lung cancer cell line 95-D induced by acutiaporberine, a novel bisalkaloid derived from Thalictrum acutifolium.
The present study demonstrates for the first time that acutiaporberine, a bisalkaloid isolated from the tranditional Chinese medical herb Thalictrum acutifolium (Hand.-Mazz.) Boivin. (TAB), induces apoptosis of a cultured highly metastatic human lung cancer cell line 95-D. Immunohistochemistry assay (IHC) and Western-blot analysis show down-regulation of bcl-2 gene and up-regulation of bax gene and c-myc gene in the treated cells. These results suggest that acutiaporberine may be a natural potential apoptosis-inducing agent for highly metastatic lung cancer.
Chen
Qi
Q
Peng
Wenlie
W
Qi
Suijian
S
Xu
Anlong
A
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001060:Aporphines; D001600:Berberine Alkaloids; D015153:Blotting, Western; D004987:Ethers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008516:Medicine, Chinese Traditional; D009362:Neoplasm Metastasis; D011518:Proto-Oncogene Proteins / Q000187:drug effects / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000187:drug effects / Q000378:metabolism; D029626:Ranunculaceae; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects / Q000378:metabolism; D051028:bcl-2-Associated X Protein
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D001060:Aporphines; C494826:BAX protein, human; D001600:Berberine Alkaloids; D004987:Ethers; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016271:Proto-Oncogene Proteins c-myc; D016159:Tumor Suppressor Protein p53; C459560:acutiaporberine; D051028:bcl-2-Associated X Protein