Ets-1 and integrin beta3 for lung metastasis from colorectal cancer.
Ets-1 functions as tissue-specific transcription factor and plays an important role in cell proliferation, differentiation, angiogenesis, and apoptosis. To elucidate the involvement of Ets-1 in lung metastasis from colorectal cancer, immunohistochemical analysis was performed on 51 colorectal cancer patients (22 with lung metastasis and 29 with advanced colorectal cancer showing no recurrence for at least 5 years after surgery). Tumorous and stromal Ets-1 immunoreactivity was evaluated. Ets-1 protein was demonstrated within stromal fibroblasts, myofibroblasts and capillaries, and also within carcinoma cells. Stromal Ets-1 immunoreactivity in primary colorectal cancer was statistically correlated with lung metastasis (p<0.05). In primary colorectal cancer, a significant association was observed between stromal Ets-1 immunoreactivity and vascular integrin beta3 expression (p<0.01). In the cases with lung metastasis, vascular integrin beta3 index in lung metastases was significantly diminished compared with primary tumors or liver metastases (p<0.01). In contrast, stromal or tumorous Ets-1 expression did not change. In a multivariate model using logistic stepwise regression analysis, vascular integrin beta3 and stromal Ets-1 overexpression were significantly and independently related to lung metastasis. Stromal Ets-1 and/or vascular integrin beta3 may be useful markers for risk of lung metastasis from colorectal cancer.
Sato
Takahiro
T
Department of Surgery, Shinminato Municipal Hospital, Japan. YIB03163@niftyserve.or.jp
Miwa
Atsuo
A
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D039661:Integrin beta3; D008175:Lung Neoplasms / Q000378:metabolism* / Q000556:secondary*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis / Q000378:metabolism*; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors / Q000096:biosynthesis / Q000378:metabolism*
D015703:Antigens, CD; C494041:ETS1 protein, human; D039661:Integrin beta3; D010980:Platelet Membrane Glycoproteins; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors