title: Distribution of polymorphic forms at the porcine GH locus in a population of day-10 pig embryos.
abstract: The present study describes an analysis of genotype and allele distribution at the porcine GH locus among day-10 pig embryos. Embryos were collected post mortem from 6 crossbred (Danish Landrace x Yorkshire) sows inseminated with mixed Duroc semen and individually frozen for later analysis. After extraction, DNA was subjected to PCR amplification and restriction analysis with Msp I and Hae II enzymes. The genotype frequencies were: Msp I CD 0.17, DD 0.83; and Hae II AA 0.33, AB 0.58; and BB 0.09. The Msp I CC genotype was not found among analysed embryos. To our knowledge, this is the first report on the genotype and allele distribution at the GH locus among early pig embryos.
authors.affiliation: Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University of Poznań, ul. Wołyńska 33, 60-637 Poznań, Poland. lechniak@jay.au.poznan.pl
authors.forename: Dorota
authors.initials: D
authors.lastname: Lechniak
authors.forename: Susan E
authors.initials: SE
authors.lastname: Long
authors.forename: Anna K
authors.initials: AK
authors.lastname: Nissen
mesh_terms: D000818:Animals; D013006:Growth Hormone / Q000235:genetics*; D011110:Polymorphism, Genetic; D013552:Swine / Q000196:embryology*
chemical_list: D013006:Growth Hormone