A fluorescence polarization assay for cyclic nucleotide phosphodiesterases.
Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of the 3'-ester bond of cyclic AMP (cAMP) and cyclic GMP (cGMP), important second messengers in the transduction of a variety of extracellular signals. There is growing interest in the study of PDEs as drug targets for novel therapeutics. We describe the development of a homogeneous fluorescence polarization assay for PDEs based on the strong binding of PDE reaction products (i.e., AMP or GMP) onto modified nanoparticles through interactions with immobilized trivalent metal cations. This assay technology (IMAP) is applicable to both cAMP- and cGMP-specific PDEs. Results of the assay in 384- and 1536-well microplates are presented.
Huang
Wei
W
Gilead Sciences Inc., Fosler City, CA, USA.
Zhang
Yan
Y
Sportsman
J Richard
JR
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002417:Cattle; D005454:Fluorescence Polarization / Q000295:instrumentation / Q000379:methods; D006868:Hydrolysis; D009712:Nucleotides, Cyclic / Q000378:metabolism
D009712:Nucleotides, Cyclic; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases