Plasma enterostatin: identification and release in rats in response to a meal.
OBJECTIVE
To discover a possible absorption and/or secretion of enterostatin into the circulating blood, as well as to compare the levels of circulating enterostatin after high-fat feeding and low-fat feeding.


METHODS
Using a specific enzyme-linked immunosorbent assay, plasma enterostatin levels were determined after feeding a high-fat, a high-fat/-sucrose, or a low-fat meal to Sprague-Dawley rats deprived of food overnight.


RESULTS
The enterostatin levels were increased by all diets; the response to the high-fat and the high-fat/-sucrose meals was greater in magnitude and duration than that to the low-fat meal. In addition, enterostatin levels correlated with the intake of dietary fat. Plasma enterostatin levels after high-fat feeding were found to be similar to those after intravenous administration of exogenous enterostatin known to inhibit high-fat food intake. Gel chromatography of pooled postprandial plasma extracts followed by high-performance liquid chromatography analysis showed that plasma enterostatin was identical to synthetic enterostatin. Affinity cross-linking of plasma proteins with 125I-enterostatin on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by autoradiography, revealed a single band with a molecular weight of about 66 kDa, indicating the presence of a potential enterostatin-binding protein in plasma.


CONCLUSIONS
The measurements of plasma enterostatin may be a sensitive indicator for the measurement of fat intake.
Mei
Jie
J
Department of Cell and Molecular Biology, University of Lund, Lund, Sweden.
Sörhede-Winzell
Maria
M
Erlanson-Albertsson
Charlotte
C
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003089:Colipases / Q000008:administration & dosage / Q000097:blood*; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage; D019422:Dietary Sucrose / Q000008:administration & dosage; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors; D005260:Female; D005502:Food; D005508:Food Deprivation; D007275:Injections, Intravenous; D011498:Protein Precursors / Q000008:administration & dosage / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley
D003089:Colipases; D004041:Dietary Fats; D019422:Dietary Sucrose; D004792:Enzyme Precursors; D011498:Protein Precursors; C037107:procolipase