title: Determination of WR-1065 and WR-33278 by liquid chromatography with electrochemical detection.
abstract: A liquid chromatographic method using electrochemical detection is presented for measuring the thiol WR-1065 12-(3-aminopropylamino) ethanethiol] and its symmetrical disulfide WR-33278 [NH2(CH2)3NHCH2CH2S]2. WR- 1065 is the active, radioprotective drug derived from the phosphorothioate pro-drug WR-2721 (amifostine). External standard curves for both compounds were linear over the range of 40-200 pmol injected (r2 = 0.999 and 0.996 for the thiol and disulfide, respectively). The detection and quantitation limits for WR-1065 were 9 and 18 pmol, respectively, whereas the corresponding values for WR-33278 were 30 and 59 pmol, respectively. Within- and between-day determinations of measurement Vision and accuracy for both compounds validated the suitability of this assay method.
authors.affiliation: Armed Forces Radiobiology Research Institute, Radiation Medicine Department, Bethesda, MD 20889-5603, USA. pendergrass@afrri.usuhs.mil
authors.forename: James A
authors.initials: JA
authors.lastname: Pendergrass
authors.forename: Venkataraman
authors.initials: V
authors.lastname: Srinivasan
authors.forename: K Sree
authors.initials: KS
authors.lastname: Kumar
authors.forename: William E
authors.initials: WE
authors.lastname: Jackson
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Seed
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D008624:Mercaptoethylamines / Q000032:analysis*
chemical_list: D008624:Mercaptoethylamines; C020174:N-(2-mercaptoethyl)-1,3-diaminopropane; C045611:WR 33278