Mutation detection of the human glucocorticoid receptor alpha gene area coding for the hormone-binding domain by denaturing gradient gel electrophoresis.
Mutations in the hormone-binding domain of the human glucocorticoid receptor alpha (hGRalpha) gene have been detected in a variety of glucocorticoid resistance syndromes. Using the denaturing gradient gel electrophoresis technique, we developed a sensitive method for the detection of alterations in the gene area coding for the whole hormone-binding domain and part of the DNA-binding domain of the hGRalpha. This method can be applied for screening of glucocorticoid receptor gene alterations in glucocorticoid-dependent diseases.
Tsolakidou
A F
AF
Laboratory of Biological Chemistry, University of Athens Medical School, 75, Mikras Asias Str., GR-115 27, Athens, Greece.
Coulocheri
S A
SA
Sekeris
C E
CE
Moutsatsou
P
P
D001483:Base Sequence; D001665:Binding Sites; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005938:Glucocorticoids / Q000378:metabolism; D006801:Humans; D016296:Mutagenesis; D016133:Polymerase Chain Reaction / Q000379:methods; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; C411470:glucocorticoid receptor alpha