Genotype association of C(-735)T polymorphism in matrix metalloproteinase 2 gene with G(8002)A endothelin 1 gene with plaque psoriasis.
BACKGROUND
Excessive angiogenesis is one of the characteristic features of psoriasis.


OBJECTIVE
To determine the possible genetic background of neo-angiogenesis in plaque psoriasis, frequent polymorphisms in matrix metalloproteinase 2 (MMP-2) and endothelin 1 (ET-1) genes were studied.


METHODS
The case group (n = 119) included patients with plaque psoriasis, aged 44 +/- 15 years. The age of onset of psoriasis was 27 +/- 11 years. The control group (n = 184) consisted of healthy subjects without any individual history of psoriasis, aged 37 +/- 15 years. C(-735)T MMP-2 and G(8002)A ET-1 polymorphisms were determined by PCR reaction with subsequent restriction analyses.


RESULTS
A significant difference in genotype distribution of C(-735)T MMP-2 between psoriatic and control patients was found (p(corr) = 0.008). Two associated genotypes (CCGG and CTGG) of the two polymorphisms were significantly less frequent in psoriatic patients (p(corr) = 0.03 and p(corr) = 0.008, respectively).


CONCLUSIONS
The results seem to reflect a different susceptibility of MMP-2 as well as of some associated MMP-2 and ET-1 genotypes to psoriasis.
Vasků
V
V
Department of Dermatology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. avasku@med.muni.cz
Vasků
A
A
Tschöplová
S
S
Izakovicová Hollá
L
L
Semrádová
V
V
Vácha
J
J
D000328:Adult; D016022:Case-Control Studies; D019332:Endothelin-1 / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000235:genetics*; D011110:Polymorphism, Genetic; D011565:Psoriasis / Q000235:genetics*
D019332:Endothelin-1; D020778:Matrix Metalloproteinase 2