title: Purification and characterization of a lipase from the glycolipid-producing yeast Kurtzmanomyces sp. I-11.
abstract: An extracellular lipase produced by the glycolipid-producing yeast Kurtzmanomyces sp. I-11 was purified by ammonium sulfate precipitation and column chromatographies on DEAE-Sephadex A-25, SP-Sephadex C-50, and Sephadex G-100. Based on the analysis of the purified lipase on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified lipase was judged to be homogeneous and its molecular mass was estimated to be approximately 49 kDa. The optimum temperature for the activity was 75 degrees C, and the activity was very stable at temperatures below 70 degrees C. The active pH range of this lipase was 1.9-7.2, and the activity was stable at pH below 7.1. The lipase showed a preference for C18 acyl groups by measurements with p-nitrophenyl esters and triglycerides as substrates. The lipase was very stable in the presence of various organic solvents at a concentration of 40%. Although the N-terminal sequence of the Kurtzmanomyces lipase was very similar to that of lipase A from Candida antarctica, the pH profiles of the two lipases were significantly different.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan. kakugawa@syokuhin-kg.pref.hiroshima.jp
authors.forename: Koji
authors.initials: K
authors.lastname: Kakugawa
authors.forename: Megumi
authors.initials: M
authors.lastname: Shobayashi
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
authors.forename: Tokichi
authors.initials: T
authors.lastname: Miyakawa
mesh_terms: D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006017:Glycolipids / Q000096:biosynthesis*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D012997:Solvents; D013379:Substrate Specificity
chemical_list: D006017:Glycolipids; D012997:Solvents; D008049:Lipase