A49T, V89L and TA repeat polymorphisms of steroid 5alpha-reductase type II and breast cancer risk in Japanese women.
BACKGROUND
Breast cancer is hormone related, as are cancers of the endometrium, ovary, and prostate. Several studies have suggested that higher extracellular levels of androgens are associated with breast cancer risk, while biological evidence indicates that androgens are protective. The codon 49 alanine to threonine substitution (A49T), codon 89 valine to leucine substitution (V89L) and TA repeat polymorphisms of the steroid 5alpha-reductase type II (SRD5A2) gene are considered functional with respect to enzyme activity converting testosterone into dihydrotestosterone. To test the hypothesis that these three polymorphisms are associated with risk of breast cancer, a case-control study was conducted with patients of Aichi Cancer Center Hospital.


METHODS
The cases were 237 patients histologically diagnosed with breast cancer, and the controls were 185 noncancer outpatients. DNA from peripheral blood was genotyped by PCR methods.


RESULTS
The threonine allele of A49T was not found in our subjects. Compared with the V/V genotype of V89L, the L/L genotype was associated with a decreased risk (crude odds ratio [OR] = 0.61, 95% confidence interval [CI] = 0.36-1.05). This was also the case for the TA(9/9) genotype, with an OR of 0.58 (95% CI = 0.13-2.63) relative to TA(0/0). Among women with the TA(0/0) genotype, however, the OR for the L/L genotype was 0.46 (95% CI = 0.24-0.88) compared with the V/V genotype, and those with the V/V and TA(0/0) genotypes had the highest risk. The haplotype with the L and TA(9) repeat alleles was not found.


CONCLUSIONS
This study is the first to our knowledge focusing on Japanese women, suggesting that SRD5A2 polymorphisms might have an association with breast cancer risk. Further large-sample studies will be required to confirm the association and to assess any interactions with environmental factors.
Yang
Chunxia
C
Division of Epidemiology and Prevention, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan.
Hamajima
Nobuyuki
N
Iwata
Hiroji
H
Saito
Toshiko
T
Matsuo
Keitaro
K
Hirose
Kaoru
K
Inoue
Manami
M
Takezaki
Toshiro
T
Tajima
Kazuo
K
D000328:Adult; D017668:Age of Onset; D000428:Alcohol Drinking / Q000453:epidemiology; D000483:Alleles; D019943:Amino Acid Substitution; D000728:Androgens / Q000502:physiology; D015992:Body Mass Index; D001943:Breast Neoplasms / Q000453:epidemiology / Q000235:genetics*; D042944:Cholestenone 5 alpha-Reductase; D003062:Codon / Q000235:genetics*; D004967:Estrogens; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008593:Menopause; D008875:Middle Aged; D018598:Minisatellite Repeats; D020125:Mutation, Missense; D009376:Neoplasms, Hormone-Dependent / Q000453:epidemiology / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics*; D017354:Point Mutation; D017584:Reproductive History; D012306:Risk; D012307:Risk Factors; D012907:Smoking / Q000453:epidemiology
D000728:Androgens; D003062:Codon; D004967:Estrogens; D010088:Oxidoreductases; D042944:Cholestenone 5 alpha-Reductase