[Short tandem repeat loci examination after repair of coracoclavicular ligament injury by tissue engineered tendon].
OBJECTIVE
To evaluate the result of clinical application in the repair of coracoclavicular ligament injury by tissue engineered tendon using the technique of short tandem repeat loci examination.


METHODS
In september 1999, human embryonic tendon cells and artificial materials were co-cultured in vitro to construct tissue engineered tendon, which repaired coracoclavicular ligament injury. After 6 months of operation, micro-tissue were sampled during the operation of removal of internal fixation, and morphological characteristics were examined by HE staining, DNA of tissues were extracted to examine D3S1754 and Cyar04 gene loci.


RESULTS
The shoulder function of the patient was recovered well after operation, and no local or systemic immunological rejection were occurred. The electrophoresis typing showed 13/14 at D3S1754 and 8/9 at Cyar04 in the tissue of tissue engineered tendon, while the autogenous ligament were 13/13 and 8/8 at D3S1754 and Cyar04 loci respectively, which suggested that the tissue engineered tendon was survived in vivo.


CONCLUSIONS
The examination of short tandem repeat loci is a better index to evaluate the survival situation of tissue engineered tissue after transplantation in clinical application.
Xie
H Q
HQ
Department of Orthopedic Surgery, First University Hospital, West China University of Medical Sciences, Chengdu, Sichuan, P. R. China 610041.
Yang
Z M
ZM
Xin
J P
JP
D000173:Acromioclavicular Joint / Q000293:injuries*; D000328:Adult; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005333:Fetus; D006801:Humans; D004204:Joint Dislocations / Q000150:complications; D008023:Ligaments, Articular / Q000293:injuries*; D008297:Male; D019736:Prostheses and Implants; D020080:Tandem Repeat Sequences; D013710:Tendons / Q000737:chemistry / Q000166:cytology* / Q000637:transplantation; D023822:Tissue Engineering
D004247:DNA