title: A syntenin-like protein with postsynaptic density protein (PDZ) domains produced by black tiger shrimp Penaeus monodon in response to white spot syndrome virus infection.
abstract: We report the isolation and characterization of products from a subtractive cDNA library from the haemolymph of Penaeus monodon experimentally infected with white spot syndrome virus (WSSV). One cDNA derived from up-regulated mRNA was identified. A homology search indicated similarity to the putative protein syntenin (TE8). The nearly complete nucleotide sequence of TE8 was obtained by rapid amplification of cDNA (RACE). Its putative protein product contained a tandem repeat of PDZ domains (postsynaptic density protein or PSD-95, DlgA and ZO-1). We propose that TE8 may function as an adapter that couples PDZ-binding protein(s) in a signaling pathway involved in the shrimp response to WSSV.
authors.affiliation: Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkla, Thailand.
authors.forename: Phuwadol
authors.initials: P
authors.lastname: Bangrak
authors.forename: Potchanapond
authors.initials: P
authors.lastname: Graidist
authors.forename: Wilaiwan
authors.initials: W
authors.lastname: Chotigeat
authors.forename: Kidchakan
authors.initials: K
authors.lastname: Supamattaya
authors.forename: Amornrat
authors.initials: A
authors.lastname: Phongdara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D004267:DNA Viruses / Q000502:physiology*; D018076:DNA, Complementary / Q000032:analysis / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D005784:Gene Amplification; D015967:Gene Expression Regulation, Viral; D015723:Gene Library; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D033561:Penaeidae / Q000378:metabolism / Q000821:virology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D015398:Signal Transduction; D053676:Syntenins
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D004279:DNA, Viral; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C109621:SDCBP protein, human; D053676:Syntenins; C033652:postsynaptic density proteins