title: Achievement of pregnancy in globozoospermia with Y chromosome microdeletion after ICSI.
abstract: Pregnancy achieved with sperm from a patient with globozoospermia is rare, even after ICSI, since the activation of the oocyte may not occur in this disorder. Therefore, activation of the oocytes by piezoelectricity or calcium ionophores has been suggested, although spontaneous activation of the oocyte after ICSI has been reported in some cases. We report a successful pregnancy in a couple in which the male partner had globozoospermia with microdeletions in the Y chromosome with no further assisted activation after ICSI. During the diagnostic study of the husband, increased numerical chromosome abnormalities after fluorescent in-situ hybridization (FISH) and microdeletions in AZFa; sY86 and AZFb; sY 131 were detected. Out of the 13 oocytes injected, four fertilized and a twin pregnancy was obtained after replacement of four embryos. Healthy twin girls were delivered after a term pregnancy. Some patients with globozoospermia may also have Y chromosome microdeletions, which subsequently may be inherited by the male offspring in cases of achievement of pregnancy.
authors.affiliation: Department of Obstetrics and Gynaecology, Division of Reproductive Medicine, Baskent University Kubilay Sok, 36 Maktepo, Ankara 06570, Turkey. hnzeynelog@e-kolay.net
authors.forename: Hulusi Bulent
authors.initials: HB
authors.lastname: Zeyneloglu
authors.forename: Volkan
authors.initials: V
authors.lastname: Baltaci
authors.forename: Hakan Eyup
authors.initials: HE
authors.lastname: Duran
authors.forename: Esra
authors.initials: E
authors.lastname: Erdemli
authors.forename: Sertac
authors.initials: S
authors.lastname: Batioglu
mesh_terms: D000328:Adult; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004624:Embryo Transfer; D005260:Female; D017353:Gene Deletion; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D010062:Ovulation Induction; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000002:abnormalities*