Genetic vaccines protect against Sin Nombre hantavirus challenge in the deer mouse (Peromyscus maniculatus).
We used a deer mouse (Peromyscus maniculatus) infection model to test the protective efficacy of genetic vaccine candidates for Sin Nombre (SN) virus that were known to provoke immunological responses in BALB/c mice (Bharadwaj et al., Vaccine 17, 2836-2843, 1999 ). Protective epitopes were localized in each of four overlapping cDNA fragments that encoded portions of the SN virus G1 glycoprotein antigen; the nucleocapsid gene also was protective. The protective efficacy of glycoprotein gene fragments correlated with splenocyte proliferation in the presence of cognate antigen, but none induced neutralizing antibodies. Genetic vaccines against SN virus can protect outbred deer mice from infection even in the absence of a neutralizing antibody response.
Bharadwaj
Mausumi
M
Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
Mirowsky
Katy
K
Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
Ye
Chunyan
C
Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
Botten
Jason
J
Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
Masten
Barbara
B
Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
Yee
Joyce
J
Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
Lyons
C Richard
CR
Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
Hjelle
Brian
B
Center for Emerging Infectious Diseases, Departments of Pathology1, Medicine2 and Biology and Molecular Genetics and Microbiology3, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D018076:DNA, Complementary; D018778:Hantavirus Infections / Q000276:immunology / Q000517:prevention & control*; D007273:Injections, Intramuscular; D051379:Mice; D019251:Nucleocapsid / Q000235:genetics / Q000276:immunology; D010542:Peromyscus; D029261:Sin Nombre virus / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage*
D000914:Antibodies, Viral; D018076:DNA, Complementary; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C104008:glycoprotein G1, Hantavirus