Modeling the pathways of energy balance using the N1E-115 murine neuroblastoma cell line.
A good in vitro model within which to investigate molecular interactions between feeding relevant neuropeptide systems has been lacking. Consequently, we began using reverse transcriptase-polymerase chain reaction (RT-PCR) to screen various neuronal cell lines for the presence of feeding relevant neuropeptides and receptors. N1E-115 murine neuroblastoma cells have emerged as an attractive candidate for further analysis because they contain mRNA for a variety of key systems implicated in the regulation of energy homeostasis.
Roth
Jonathan D
JD
Department of Animal Biology, School of Vetrinary Medicine and the Institute of Neurological Sciences, University of Pennsylvania, Philadelphia, PA 19104, USA. jdroth@vet.upenn.edu
Yee
Daniel K
DK
Kisley
Lori R
LR
Fluharty
Steven J
SJ
D054369:Agouti-Related Protein; D000818:Animals; D004734:Energy Metabolism / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins; D020738:Leptin / Q000235:genetics; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics; D009447:Neuroblastoma; D009478:Neuropeptide Y / Q000235:genetics; D009479:Neuropeptides / Q000235:genetics*; D011333:Pro-Opiomelanocortin / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D011972:Receptor, Insulin / Q000235:genetics; D044104:Receptor, Melanocortin, Type 3; D044105:Receptor, Melanocortin, Type 4; D018043:Receptors, Corticotropin / Q000235:genetics; D012097:Repressor Proteins; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D014157:Transcription Factors; D014407:Tumor Cells, Cultured / Q000378:metabolism; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
D054369:Agouti-Related Protein; D036341:Intercellular Signaling Peptides and Proteins; D020738:Leptin; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D009479:Neuropeptides; D011506:Proteins; D012333:RNA, Messenger; D044104:Receptor, Melanocortin, Type 3; D044105:Receptor, Melanocortin, Type 4; D018043:Receptors, Corticotropin; D012097:Repressor Proteins; C491411:Socs3 protein, mouse; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D014157:Transcription Factors; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D011333:Pro-Opiomelanocortin; D011972:Receptor, Insulin