Identification of three genes up-regulated in PU.1 rescued monocytic precursor cells.
The requirement of the transcription factor PU.1 for macrophage development has been well documented. However, the target genes regulated by PU.1 controlling macrophage maturation are not known. A granulocyte macrophage colony stimulating factor (GM-CSF)-dependent PU.1 null monocytic precursor cell was stably transduced with a PU.1-expressing retrovirus. The expression of PU.1 altered the surface expression of a few proteins expressed on monocytes; these cells, however, remained GM-CSF dependent and maintained an immature phenotype. In contrast to the PU.1 null cells, the cells expressing PU.1 responded to macrophage colony stimulating factor (M-CSF) with subsequent development into mature macrophages. Using suppressive subtractive hybridization between the PU.1 null and immature PU.1 rescued cells, three genes, MRP-14, Dap12 and CD53, were found expressed in the rescued cells, but not in the PU.1 null cells. In addition, these genes were modulated during M-CSF-induced maturation of the PU.1 rescued cells. The PU.1 null and rescued early monocytic cells provide a useful model to study the role of PU.1 in macrophage development.
Henkel
Gregory W
GW
The Burnham Institute, 10901 N. Torrey Pines Road, La Jolla, CA 92037, USA.
McKercher
Scott R
SR
Maki
Richard A
RA
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D040502:Calgranulin B / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008565:Membrane Proteins; D009000:Monocytes / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D011971:Receptors, Immunologic / Q000235:genetics* / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics; D060265:Tetraspanin 25; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic; D015854:Up-Regulation; D000099232:Proto-Oncogene Protein Spi-1
D048868:Adaptor Proteins, Signal Transducing; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D040502:Calgranulin B; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D012313:RNA; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D060265:Tetraspanin 25; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C554894:CD53 protein, human; C110546:TYROBP protein, human