Synthesis and oxidative refolding of hainantoxin-IV.
Hainantoxin-IV, a neutoxic peptide from the spider Selenocosimia hainana, was synthesized by solid-phase method with fluorenylmethyoxycarbonyl amino acids (Fmoc-AA). Reverse-phase HPLC was used to monitor the oxidative folding of synthetic Hainantoxin-IV under different reaction conditions in order to find optimal conditions for renaturation of synthetic Hainantoxin-IV. The best renaturation yield was received in 5 mmol/L GSH and 0.5 mmol/L GSSG at pH 8.0 in 0.1 mol/L Tris-HCl and 0.1 mol/L NaCl buffer. The renaturated Hainantoxin-IV was monitored with MALDI-TOF MS  reverse-phase HPLC and isolated mouse phrenic nerve-diaphragm preparation.
Liu
Zhong-Hua
ZH
College of Life Science of Hunan Normal University, Changsha 410081, China. liangsp@public.cs.hn.cn
Chen
Ping
P
Liang
Song-Ping
SP
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003964:Diaphragm / Q000294:innervation; D051379:Mice; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry* / Q000494:pharmacology; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology; D010791:Phrenic Nerve / Q000187:drug effects; D017510:Protein Folding; D020673:Protein Renaturation; D013111:Spider Venoms / Q000737:chemistry* / Q000494:pharmacology
D009498:Neurotoxins; D010455:Peptides; D013111:Spider Venoms