Analysis of herpes simplex virus entering into cells of oral origin.
The entry of herpes simplex virus (HSV) into an oral epithelial cell line, primary normal human oral keratinocytes (NHOK) and gingival fibroblasts (GF) was examined. Infection of these cells by HSV-1 and HSV-2 was blocked by heparin. Further examination indicated that heparin reduced viral attachment but not penetration. Moreover, neomycin inhibited HSV-1 infection more effectively than HSV-2 infection in GF, but not in NHOK. In conclusion, our results elucidated some aspects of the HSV entry process into oral cells and revealed some differences in HSV entering into NHOK and GF.
Hung
Shan Ling
SL
Institute of Oral Biology, National Yang-Ming University, No. 155, Sec. 2, Li-Nong Street, Pei-Tou, Taipei, Taiwan. slhung@ym.edu.tw
Wang
Yi Hui
YH
Chen
Hsiao Wang
HW
Lee
Pei Lun
PL
Chen
Yen Ting
YT
D000818:Animals; D002522:Chlorocebus aethiops; D005347:Fibroblasts / Q000821:virology*; D006801:Humans; D015603:Keratinocytes / Q000821:virology*; D009061:Mouth Mucosa / Q000821:virology; D018139:Simplexvirus / Q000502:physiology*; D014709:Vero Cells; D010948:Viral Plaque Assay