[Methods for detection and characterization of mechanical resistance of erythrocytes in healthy men (review of literature)].
Published reports on methods for evaluation of erythrocyte rheology and mechanical resistance are reviewed. The processes running in red blood cells under the effect of mechanical factors are described. An original method for studies of vibration resistance of erythrocytes is proposed. It allows evaluation of the erythrocyte hemolysis, aggregation, and fragmentation. The method consists in combined vibroexposure of whole capillary blood in vitro (including blood collected under conditions of redistribution of erythropoiesis after exercise) and differentiated cytometry by conductometric hematological scintillators with plotting a histogram of erythrocyte distribution by volume. The method was used by many scientists who obtained different results in examinations of normal subjects. This disagreement of results of different studies is explained by numerous causes, some of which can hardly be taken into consideration. The authors attempted to standardize the method and normal (reference) values. The method is sensitive to various factors (even low intensive) affecting the erythrocyte population and allows the detection of diseases at the preclinical stage and evaluation of the degree of subject's training.
Seleznev
A V
AV
Nenashev
A A
AA
Kondurtsev
V A
VA
D002454:Cell Differentiation; D019411:Clinical Laboratory Techniques; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology*; D006801:Humans; D013314:Stress, Mechanical