Fluorescent 1,10-phenanthroline-containing oligonucleotides distinguish between perfect and mismatched base pairing.
[structure: see text] A fluorescent deoxyuridine analogue is sensitive to the polarity of its environment and exhibits a distinct emission profile in single- vs double-stranded oligonucleotides. Emission-monitored denaturation curves of internally modified dU(phen) duplexes are characteristic of the base opposite dU(phen) and distinguish between perfect and mismatched complementary oligonucleotides.
Hurley
Dennis J
DJ
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0358, USA.
Seaman
Susan E
SE
Mazura
Jan C
JC
Tor
Yitzhak
Y
D020137:Base Pair Mismatch / Q000235:genetics*; D020029:Base Pairing / Q000235:genetics*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D010618:Phenanthrolines / Q000138:chemical synthesis / Q000737:chemistry*
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D015345:Oligonucleotide Probes; D010618:Phenanthrolines