title: Porcine reproductive and respiratory syndrome (PRRS) with special reference to clinical aspects and diagnosis. A review.
abstract: After a short introduction on Porcine Reproductive and Respiratory Syndrome (PRRS) regarding the history, the first occurrence in several countries, and the causal virus, designated Lelystad virus, a description is given of the clinical aspects and several diagnostic methods. After some general remarks on the clinical aspects, the epidemic and the endemic phase of the disease are described. Regarding the diagnosis, special attention is paid to the detection of antibodies and of the PRRS Virus (PRRSV). Regarding the detection of antibodies, a description is given of three tests: the immunoperoxidase monolayer assay, the enzyme-linked immunosorbent assay, and the serum neutralization test. Concerning the detection of PRRSV, attention is paid to the isolation of the virus, the demonstration of PRRSV antigens in frozen or fixed tissue using immunohistochemistry or immunofluorescence, the in situ hybridisation technique and the Polymerase Chain Reaction (PCR).
authors.affiliation: Institute for Animal Science and Health, Lelystad, The Netherlands. g.nodelijk@id.wag-ur.nl
authors.forename: G
authors.initials: G
authors.lastname: Nodelijk
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D009500:Neutralization Tests / Q000662:veterinary; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology / Q000302:isolation & purification*; D013552:Swine
chemical_list: D000914:Antibodies, Viral