title: Glycine betaine transmethylase mutant of Pseudomonas aeruginosa.
abstract: The gene for glycine betaine transmethylase (gbt) was identified in Pseudomonas aeruginosa strain Fildes III by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout gene corresponded to an open reading frame (ORF) named PA3082 in the genome of P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in gbt blocked the utilization of choline and glycine betaine as carbon and nitrogen sources.
authors.affiliation: Departmento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5.800 Río Cuarto, Argentina.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Serra
authors.forename: Javier F
authors.initials: JF
authors.lastname: Mariscotti
authors.forename: José L
authors.initials: JL
authors.lastname: Barra
authors.forename: Gloria I
authors.initials: GI
authors.lastname: Lucchesi
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Domenech
authors.forename: Angela T
authors.initials: AT
authors.lastname: Lisa
mesh_terms: D001622:Betaine / Q000378:metabolism*; D002794:Choline / Q000378:metabolism*; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D016366:Open Reading Frames; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D001622:Betaine; D008780:Methyltransferases; D002794:Choline