title: The groESL operon of the halophilic lactic acid bacterium Tetragenococcus halophila.
abstract: The groESL operon of the halophilic lactic acid bacterium Tetragenococcus halophila was cloned by a PCR-based method. The molecular masses of GroES and GroEL proteins were calculated to be 10,153 and 56,893 Da, respectively. The amount of groESL mRNA was increased 3.8-fold by heat shock (45 degrees C), and 4-fold by high NaCl (3-4 M). The Bacillus subtilis sigmaA-like constitutive promoter existed in front of groES, and was used under both normal and stress (heat shock and high salinity) conditions.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Fukuda
authors.forename: Maki
authors.initials: M
authors.lastname: Watanabe
authors.forename: Yuji
authors.initials: Y
authors.lastname: Aso
authors.forename: Kenji
authors.initials: K
authors.lastname: Sonomoto
authors.forename: Ayaaki
authors.initials: A
authors.lastname: Ishizaki
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D018833:Chaperonins / Q000235:genetics*; D004269:DNA, Bacterial; D007777:Lactobacillaceae / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C097970:GroESL protein, Bacteria; D018833:Chaperonins