[Effect of extracellular chloride concentration on deactivation kinetics of rat ClC-1 chloride channel].
The gating mechanism of ClC-1 chloride channel was studied in this paper by heteroexpression of rat wild type ClC-1 gene in Xenopus oocytes and by two-electrode voltage clamping technique. The deactivation gating kinetic parameters were obtained by applying two exponential fitting of the deactivating currents at various extracellular chloride concentrations. It was found that decrease in extracellular chloride concentration increased the fractional amplitude of fast deactivating component, and depressed the fractional amplitude of slow deactivating component accompanied by a decrease in fast and slow deactivating time constants. These results demonstrate that the deactivation kinetic parameters of ClC-1 are largely dependent on the extracellular chloride concentration, which induces changes in channel gating.
Zhang
Xiao-Dong
XD
Department of Physiology, the Fourth Military Medical University, Xi an, 710032, China. zangym@fmmu.edu.cn
Zang
Yi-Min
YM
Zhou
Shi-Sheng
SS
Zang
Wei-Jin
WJ
Yu
Xiao-Jiang
XJ
Wang
Yue-Min
YM
D000818:Animals; D018118:Chloride Channels / Q000187:drug effects* / Q000502:physiology; D002712:Chlorides / Q000494:pharmacology*; D004594:Electrophysiology; D005260:Female; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D009865:Oocytes / Q000502:physiology; D051381:Rats; D014981:Xenopus
C086321:CLC-1 channel; D018118:Chloride Channels; D002712:Chlorides