[Indirect determination of berberine hydrochloride by atomic absorption spectrometry].
A method for the indirect determination of berberine in tabellae berberine hydrochloride by ion pair extraction and atomic absorption spectrometry was described. Berberine reacted with inorganic complex tetrathiocyanate cobalt(II) to form a stable ion pair associated complex at certain pH value. The ion pair associated complex was extracted with organic solvent and then transferred into aqueous solution. The content of cobalt in the solution was measured by flame atomic absorption spectrometry and the amount of berberine hydrochloride was calculated according to the content of cobalt. The result of the present method agreed with pharmacopeia method. This method is sensitive, dependable, precise, and applicable.
Deng
S
S
Analytical Testing Center, Hunan Medical University, Changsha 410078.
Li
X
X
D001599:Berberine / Q000032:analysis*; D013054:Spectrophotometry, Atomic / Q000379:methods
D001599:Berberine