The SH3, HOOK and guanylate kinase-like domains of hDLG are important for its cytoplasmic localization.
BACKGROUND
hDLG, the human homologue of the Drosophila tumour suppressor dlg, functions as a scaffolding protein that facilitates the transmission of diverse downstream signals. hDLG possesses multiple protein-binding domains, including three PDZ domains, an SH3 domain, a HOOK domain and a guanylate kinase-like (GK) domain.


RESULTS
We studied the significance of the PDZ, SH3, HOOK and GK domains in the cytoplasmic localization of hDLG. We found that mutation of the SH3 or GK domain, but not the PDZ domain, resulted in a re-localization of hDLG to the nucleus. Furthermore, hDLG was found to possess a potential nuclear localization signal in the HOOK domain.


CONCLUSIONS
These results suggest that the SH3, HOOK and GK domains of hDLG are important for its cytoplasmic localization.
Kohu
Kazuyoshi
K
Department of Molecular and Genetic Information, Institute for Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan.
Ogawa
Fumiaki
F
Akiyama
Tetsu
T
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism*; D004251:DNA Transposable Elements; D000074288:Discs Large Homolog 1 Protein; D029721:Drosophila Proteins; D051528:Guanylate Kinases; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D009703:Nucleoside-Phosphate Kinase / Q000737:chemistry; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D033921:Vesicular Transport Proteins; D018909:src Homology Domains
D048868:Adaptor Proteins, Signal Transducing; D000596:Amino Acids; C513314:DLG1 protein, human; D004251:DNA Transposable Elements; D000074288:Discs Large Homolog 1 Protein; D029721:Drosophila Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; D011506:Proteins; D033921:Vesicular Transport Proteins; C100411:hook protein, Drosophila; D009703:Nucleoside-Phosphate Kinase; D051528:Guanylate Kinases