title: Dietary genistein affects brain protein synthesis rates in ovariectomized female rats.
abstract: The purpose of this study was to determine whether genistein affects the rate of brain protein synthesis in ovariectomized female rats. Experiments were conducted on three groups of 12-wk-old female rats: those in group 1 were ovariectomized to reduce the level of plasma sex hormone; those in group 2 were ovariectomized and fed diets containing 0.01% genistein; and those in group 3 were sham-operated controls. The fractional rates of protein synthesis in the brain of ovariectomized rats fed genistein were significantly greater than those in ovariectomized rats without genistein treatment. In the cerebral cortex and cerebellum, the RNA activity [g protein synthesized/(g RNA.d)] significantly correlated (r > 0.86, P < 0.001) with the fractional rate of protein synthesis. The RNA concentration (mg RNA/g protein) was not related to the fractional rate of protein synthesis in any organ. The results suggest that the addition of genistein to the diet of ovariectomized female rats is likely to increase the rate of protein synthesis in the brain, and that RNA activity is at least partly related to the fractional rate of brain protein synthesis.
authors.affiliation: Department of Home Economics, Aichi University of Education, Kariya, Japan.
authors.forename: Sunok
authors.initials: S
authors.lastname: Lyou
authors.forename: Emi
authors.initials: E
authors.lastname: Hirano
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Tujioka
authors.forename: Yuka
authors.initials: Y
authors.lastname: Mawatari
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Hayase
authors.forename: Satoshi
authors.initials: S
authors.lastname: Okuyama
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Yokogoshi
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D004032:Diet; D005260:Female; D019833:Genistein / Q000008:administration & dosage* / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D010052:Ovariectomy; D012313:RNA / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D014018:Tissue Distribution
chemical_list: D009419:Nerve Tissue Proteins; D012313:RNA; D019833:Genistein