title: Determination of nicotinamide and 4-aminobenzoic acid in pharmaceutical preparation by LC.
abstract: A rapid, precise and time saving high performance liquid chromatographic method has been developed and validated for the simultaneous determination of nicotinamide and 4-aminobenzoic acid in pharmaceutical preparation. The method involves isocratic elution of mobile phase through column in a reverse phase chromatography with UV detection at 254 nm. The ranges of quantification for nicotinamide and 4-aminobenzoic acid were 11-34 microg ml(-1) and 37-113 microg ml(-1), respectively. Investigating specificity, linearity, precision, accuracy, robustness and ruggedness performed the validation of the method.
authors.affiliation: HEJ Research Institute of Chemistry, International Center for Chemical Sciences, University of Karachi, 75270, Karachi, Pakistan.
authors.forename: Abdul Rafiq
authors.initials: AR
authors.lastname: Khan
authors.forename: Khalid Mohammad
authors.initials: KM
authors.lastname: Khan
authors.forename: Shahnaz
authors.initials: S
authors.lastname: Perveen
authors.forename: Naeem
authors.initials: N
authors.lastname: Butt
mesh_terms: D010129:4-Aminobenzoic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D009536:Niacinamide / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D004338:Drug Combinations; D009536:Niacinamide; D010129:4-Aminobenzoic Acid