[The effect of combined treatment of recombinant human growth hormone and insulin-like growth factor-1 on wound healing and protein catabolism in burned rats].
OBJECTIVE
To investigate the effect of combined treatment of recombinant human growth hormone (rhGH) and insulin-like growth factor-1 (IGF-1) on wound healing and protein catabolism in burned rats.


METHODS
Forty Wistar rats with deep II degree scald injury were divided randomly into four groups and received rhGH (0.1 U/kg.d), rhGH (0.1 U/kg.d) plus IGF-1 (2.0 mg/kg.d), IGF-1 (2.0 mg/kg.d) and Ringer's solution (2 ml/kg.d, as control group) respectively. The wound healing time and protein catabolism levels of every groups were compared after 2 weeks.


RESULTS
Total body weight began to increase after 2 weeks in rhGH group and rhGH plus IGF-1 group, but in control group, it was occurred after 4-5 weeks. The body weight of rhGH plus IGF-1 group was 1.65 times than that of rhGH group. The wound healing time in rhGH plus IGF-1 group (17.1 +/- 4.4) days was significantly lower than that of rhGH (20.5 +/- 4.8) days and control group (29.7 +/- 6.3) days. The protein level of rhGH plus IGF-1 group was significantly higher than that of control group and rhGH group.


CONCLUSIONS
It suggests that rhGH plus IGF-1 with synergism is more effective in promoting wound healing and increasing the protein catabolism.
Chen
H D
HD
Department of Burn Surgery, Guangdong Provincial People's Hospital, Guangzhou, Guangdong, P. R. China 510080.
Xie
J L
JL
Lai
W
W
D000818:Animals; D002056:Burns / Q000378:metabolism / Q000628:therapy*; D006133:Growth Substances / Q000627:therapeutic use*; D007334:Insulin-Like Growth Factor I / Q000627:therapeutic use*; D008297:Male; D011506:Proteins / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects*
D006133:Growth Substances; D011506:Proteins; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I