Development and characterization of monoclonal antibodies to chicken interleukin-15.
The chicken IL-15 gene was recently cloned and shown to encode a polypeptide with T cell growth factor activity similar to IL-2. To further characterize the chemical and biological properties of chicken IL-15, we generated a panel of monoclonal antibodies against bacterially expressed protein and characterized their binding specificities. All antibodies were reactive by ELISA with recombinant IL-15, but not IL-2, and identified a 15kDa recombinant chicken IL-15 by Western blot analysis. Two antibodies inhibited IL-15-induced proliferation of splenic lymphoblast cells. These monoclonal antibodies will be useful for further structural and immunological studies of chicken IL-15.
Min
Wongi
W
Parasite Biology, Epidemiology and Systematics Laboratory, US Department of Agriculture, Animal and Natural Resources Institute, Beltsville, MD 20705, USA.
Lillehoj
Hyun S
HS
Li
Guangxing
G
Sohn
Eun J
EJ
Miyamoto
Tadashi
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D019556:COS Cells; D002455:Cell Division / Q000276:immunology; D002645:Chickens / Q000276:immunology*; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D019409:Interleukin-15 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011994:Recombinant Proteins; D013047:Specific Pathogen-Free Organisms
D000911:Antibodies, Monoclonal; D019409:Interleukin-15; D011994:Recombinant Proteins