title: [Sequencing and homology analysis of cDNAs encoding FhGST and AsGST].
abstract: OBJECTIVE
  To study the homologies between FhGST, AsGST and Sj26GST.
  
  
  METHODS
  Total RNAs were extracted from Fasciola hepatica (Fh) and Ascaris suum (As) by guanidine hydrochloride/CsCl ultra-centrifugation. Two pairs of primers were designed according to FhGST and AsGST sequences. RT-PCR was carried out using total RNA as template. PCR products were directly sequenced. Then the homology analysis was made using DNASIS software on nucleotide level.
  
  
  RESULTS
  A FhGST403 bp fragment and a AsGST411 bp fragment were obtained and they both located in the open reading frame.
  
  
  CONCLUSIONS
  On nucleotide level, the homologies are 59% and 54% between the two cloned fragments and Schistosoma japonicum 26 kDa GST (Sj26GST) respectively.
authors.affiliation: Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
authors.forename: W
authors.initials: W
authors.lastname: Sun
authors.forename: S
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D017165:Ascaris suum / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005210:Fasciola hepatica / Q000201:enzymology* / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D005982:Glutathione Transferase