[Study on the prevention of insulitis and diabetes by complete Freund's adjuvant in NOD female mice].
The degrees of insulitis, the incidence of diabetes, and the counts of alpha,beta and delta cells in islets were studied by morphological method and avidin-biotin complex immunohistochemical method in complete Freund's adjuvant(CFA) or normal saline(NS)-treated non-obese diabetic(NOD) female mice. The results showed: The incidences of in-insulitis and the scoring means were significantly lower in CFA-treated mice than those in NS-treated ones; none of 5 CFA-treated mice and 3 out of 5 NS-treated ones developed diabetes. The positive rates of alpha, and delta cells were significantly lower in CFA-treated mice than those in NS-treated ones, but the positive rate of beta cell was significantly higher in CFA-treated mice than that in NS-treated ones. The correlations were found among the scores of insulitis and the positive rates of alpha, beta, delta cells in islets (r alpha = 0.475, r beta = -0.878, r delta = 0.869). The results indicate that CFA may lessen the degrees of insulitis and the incidence of diabetes. Its effects might be related to lessen the damage of beta cell in islets.
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Research Laboratory of Hepatobiliary Diseases, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
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Zhou
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Liao
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Yang
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D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000517:prevention & control*; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000473:pathology*; D051379:Mice; D016688:Mice, Inbred NOD; D010195:Pancreatitis / Q000188:drug therapy; D011897:Random Allocation
D005620:Freund's Adjuvant