title: Collagen X expression in oviduct tissue during the different stages of the egg laying cycle.
abstract: The purpose of this investigation was to study the expression of type X collagen in the hen's oviduct. Type X collagen is a short-chain collagen that is present in the fibers of eggshell membranes, and there is evidence to suggest that it contributes to structural integrity. In situ hybridization and Northern blot analysis were used to study the expression of this important matrix constituent. The results demonstrated that gene expression was predominantly in the tubular gland cells of the isthmus segment of the oviduct. In contrast to observations with other matrix proteins, such as parathyroid hormone-related peptide and osteopontin, gene expression did not fluctuate with the position of the egg in the oviduct.
authors.affiliation: Department of Poultry Science, The Pennsylvania State University, University Park, Pennsylvania 16802-3501, USA.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: B C
authors.initials: BC
authors.lastname: Ford
authors.forename: C A
authors.initials: CA
authors.lastname: Praul
authors.forename: R M
authors.initials: RM
authors.lastname: Leach
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002645:Chickens / Q000502:physiology*; D024121:Collagen Type X / Q000096:biosynthesis*; D005260:Female; D005786:Gene Expression Regulation; D017403:In Situ Hybridization / Q000662:veterinary; D010057:Oviducts / Q000502:physiology*; D012098:Reproduction
chemical_list: D024121:Collagen Type X