Kupffer cell targeting by intraportal injection of the HVJ cationic liposome.
The aim of this study was to target Kupffer cells (KCs) selectively and efficiently by the intraportal injection of fusigenic cationic liposomes with hemagglutinating virus of Japan components (HVJ cationic liposomes). Phosphorothioate FITC-oligodeoxynucleotides (FITC-ODNs) encapsulated in either HVJ cationic liposomes, HVJ anionic liposomes or conventional cationic liposomes without HVJ were transferred to the rat. FITC-ODNs in HVJ cationic liposomes administered via portal vein were selectively transfected to KCs for up to 24 h with no apparent cytotoxicity at higher transfection efficiency than FITC-ODNs in conventional cationic liposomes without HVJ administered via portal vein or tail vein. On the other hand, FITC-ODNs in HVJ anionic liposomes were observed mainly in hepatocytes, not KCs. This new method will be useful for the modulation of KCs activity in both basic research and clinical applications.
Yoshida
Masanori
M
Department of Gastroenterological Surgery, Kyoto University, Graduate School of Medicine, 54 Kawaracho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. yosy@kuhp.kyoto-u.ac.jp
Yamamoto
N
N
Uehara
T
T
Terao
R
R
Nitta
T
T
Harada
N
N
Hatano
E
E
Iimuro
Y
Y
Yamaoka
Y
Y
D000818:Animals; D002460:Cell Line; D016650:Fluorescein-5-isothiocyanate; D018014:Gene Transfer Techniques; D007275:Injections, Intravenous; D007728:Kupffer Cells / Q000187:drug effects* / Q000201:enzymology; D007763:Lac Operon; D008081:Liposomes; D008297:Male; D008856:Microscopy, Fluorescence; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage* / Q000235:genetics; D011169:Portal Vein; D051381:Rats; D017208:Rats, Wistar; D029082:Sendai virus / Q000235:genetics*; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D001616:beta-Galactosidase; D016650:Fluorescein-5-isothiocyanate