Cloning a cDNA encoding an alternatively spliced protein of BRCA2-associated factor 35.
Inheritance of mutations in the breast cancer susceptibility gene, BRCA2, predisposes humans to breast and ovarian cancers. Inherited mutations in the BRCA2 gene are also known to cause susceptibility to prostate cancer. BRCA2 protein exists in a large multi-protein complex from which a novel structural DNA binding protein BRCA2-associated factor 35 (BRAF35) has been isolated. We have cloned a novel cDNA encoding an alternatively spliced protein of BRAF35, designated as BRAF25. BRAF25 transcript is present in various human cells. We have precisely mapped the BRAF25 cDNA sequence to the genomic chromosome 19 sequence. Analysis of the predicted sequence of BRAF25 identified a protein of 215 amino acids. BRAF25 contains a truncated high mobility group domain, a kinesin-like coiled-coil domain and multiple Src homology 2 (SH2) motifs. Western blot analysis using antibodies specific for BRAF25 revealed the presence of BRAF25 in human prostate cancer cells.
Wang
Chiang
C
Department of Urology, University of Tennessee, 956 Court Avenue, Coleman Building Room H202, Memphis, TN 38163, USA. cwang@utmem.edu
McCarty
Ida M
IM
Balazs
Louisa
L
Li
Yi
Y
Steiner
Mitchell S
MS
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000918:Antibody Specificity; D001483:Base Sequence; D002460:Cell Line; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D006609:High Mobility Group Proteins; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011471:Prostatic Neoplasms / Q000378:metabolism; D017434:Protein Structure, Tertiary; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C000625244:HMG20B protein, human; D006609:High Mobility Group Proteins