title: Prognostic value of quantitative Kaposi sarcoma--associated herpesvirus load in posttransplantation Kaposi sarcoma.
abstract: Organ transplant recipients have a higher risk of Kaposi sarcoma (KS). A quantitative real-time polymerase chain reaction assay was developed to evaluate KS-associated herpesvirus (KSHV) as a prognostic tool in transplant recipients with KS. Forty-three patients who developed KS after transplantation were included in a cross-sectional study to correlate virus load with transplantation or KS parameters. Seventeen patients (40%) had KSHV viremia (>100 copies/microg of DNA; median, 6067 copies/microg of DNA). Factors associated with these levels of viremia by univariate analysis were progression of KS (P=.00002), time from KS diagnosis (P=.0007), actual stage of KS (P=.006), initial stage of KS (P=.22), graft loss (P=.013), and time from transplantation (P=.0246). Disease progression remained associated with KSHV viremia in a multivariate analysis (P=.01). Thus, quantification of KSHV load in peripheral blood mononuclear cells could represent a useful tool for monitoring transplant recipients with KS.
authors.affiliation: Laboratory of Pharmacology, Hôpital Saint-Louis, Paris, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Pellet
authors.forename: Sylvie
authors.initials: S
authors.lastname: Chevret
authors.forename: Camille
authors.initials: C
authors.lastname: Francès
authors.forename: Sylvie
authors.initials: S
authors.lastname: Euvrard
authors.forename: Mylène
authors.initials: M
authors.lastname: Hurault
authors.forename: Christophe
authors.initials: C
authors.lastname: Legendre
authors.forename: Sophie
authors.initials: S
authors.lastname: Dalac
authors.forename: Dominique
authors.initials: D
authors.lastname: Farge
authors.forename: Corinne
authors.initials: C
authors.lastname: Antoine
authors.forename: Christian
authors.initials: C
authors.lastname: Hiesse
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Peraldi
authors.forename: Philippe
authors.initials: P
authors.lastname: Lang
authors.forename: Didier
authors.initials: D
authors.lastname: Samuel
authors.forename: Yvon
authors.initials: Y
authors.lastname: Calmus
authors.forename: Félix
authors.initials: F
authors.lastname: Agbalika
authors.forename: Patrice
authors.initials: P
authors.lastname: Morel
authors.forename: Fabien
authors.initials: F
authors.lastname: Calvo
authors.forename: Céleste
authors.initials: C
authors.lastname: Lebbé
mesh_terms: D015331:Cohort Studies; D003430:Cross-Sectional Studies; D004279:DNA, Viral; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016030:Kidney Transplantation; D009000:Monocytes / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D011183:Postoperative Complications; D011379:Prognosis; D012514:Sarcoma, Kaposi / Q000473:pathology / Q000821:virology*; D019562:Viral Load; D014766:Viremia / Q000821:virology*
chemical_list: D004279:DNA, Viral