title: Hyalinizing clear cell carcinoma of salivary glands: a study of extracellular matrix.
abstract: Hyalinizing clear cell carcinoma (HCCC) is a recently described low-grade carcinoma of the salivary glands presenting two main histological features: clear neoplastic cells and prominent hyalinized stroma. Our aim was to characterize the composition of the hyalinized stroma in three cases of HCCC. An immuno-histochemical study using laminin and type I, III and IV collagens, fibronectin and tenascin antibodies was performed. In one case, electron microscopy study was also done. Collagen I and fibronectin were always present in tumoral stroma. Collagen III was documented in two cases and tenascin at the invasive front of the neoplasm. Collagen IV and laminin were found around neoplastic cells but not at the interstitial stroma. Foci of basal lamina reduplication and large deposits of long space collagen were observed in EM. This study demonstrates that the hyalinized stroma of HCCC is not formed by the accumulation of basement membrane-type material since it is mostly composed by collagen I and fibronectin.
authors.affiliation: Departamento de Patologia Morfológica, Instituto Português de Oncologia de Francisco Gentil, Rua Professor Lima Basto, 1099-023 Lisboa Codex, Portugal. anafpinto@mail.telepac.pt
authors.forename: Ana
authors.initials: A
authors.lastname: Félix
authors.forename: Joaninha Costa
authors.initials: JC
authors.lastname: Rosa
authors.forename: José F Moura
authors.initials: JF
authors.lastname: Nunes
authors.forename: Isabel
authors.initials: I
authors.lastname: Fonseca
authors.forename: António
authors.initials: A
authors.lastname: Cidadão
authors.forename: Jorge
authors.initials: J
authors.lastname: Soares
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000473:pathology*; D003094:Collagen; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D024141:Collagen Type IV / Q000378:metabolism; D005109:Extracellular Matrix / Q000473:pathology*; D005260:Female; D006801:Humans; D006818:Hyalin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012468:Salivary Gland Neoplasms / Q000473:pathology*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D024141:Collagen Type IV; D003094:Collagen