Discordance between the effect of modulators of calcium on staurosporine-induced apoptosis and staurosporine-induced actin degradation.
Staurosporine produced DNA fragmentation characteristic of apoptosis and a dramatic alteration of cell structure that include alterations of cytoskeletal actin and cytoplasmic condensation with vacuolization. These changes were not induced by chelerythrine, a more specific PKC inhibitor than staurosporine. The calcium chelator, BAPTA, significantly reduced staurosporine-induced DNA fragmentation but did not affect staurosporine-induced changes in cytoskeletal actin. These data suggest that DNA fragmentation and actin degradation in apoptosis, induced by staurosporine, are under different regulatory control by calcium.
Rabkin
Simon W
SW
Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. rabkin@interchange.ubc.ca
Kong
Jennifer Y
JY
D000199:Actins / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D002642:Chick Embryo; D053938:DNA Fragmentation; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D006352:Heart Ventricles / Q000166:cytology / Q000196:embryology; D019311:Staurosporine / Q000494:pharmacology*
D000199:Actins; D002614:Chelating Agents; D004533:Egtazic Acid; D019311:Staurosporine; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium