title: Emergence of a novel penicillin-nonsusceptible, invasive serotype 35B clone of Streptococcus pneumoniae within the United States.
abstract: Monitoring antibiotic-resistant pneumococcal strains not covered by the 7-valent conjugate vaccine is an important priority. The Centers for Disease Control and Prevention's Active Bacterial Core Surveillance identified 68 invasive penicillin-nonsusceptible serotype 35B (PN35B) isolates recovered from 1995 to 2001 from patients residing in the states of California, Colorado, Connecticut, Georgia, Maryland, Minnesota, New York, Oregon, Tennessee, and Texas. Nonsusceptible isolates accounted for 69% of all serotype 35B isolates recovered during this time. Twelve (18%) of the 68 PN35B isolates recovered since 1995 were obtained from pediatric patients. These 68 isolates exhibited penicillin MICs of 0.25-2 microg/mL and reduced susceptibility to cefotaxime. Representative PN35B isolates exhibited a common chromosomal macrorestriction profile and identical penicillin-binding-protein gene restriction profiles characteristic of penicillin-resistant strains, and they shared a unique 7-locus sequence type that included 3 new alleles. The mosaic pbp2b and divergent ddl sequences were suggestive of interspecies recombination at the ddl-pbp2b chromosomal region.
authors.affiliation: Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. bbeall@cdc.gov
authors.forename: Bernard
authors.initials: B
authors.lastname: Beall
authors.forename: M Catherine
authors.initials: MC
authors.lastname: McEllistrem
authors.forename: Robert E
authors.initials: RE
authors.lastname: Gertz
authors.forename: David J
authors.initials: DJ
authors.lastname: Boxrud
authors.forename: John M
authors.initials: JM
authors.lastname: Besser
authors.forename: Lee H
authors.initials: LH
authors.lastname: Harrison
authors.forename: James H
authors.initials: JH
authors.lastname: Jorgensen
authors.forename: Cynthia G
authors.initials: CG
authors.lastname: Whitney
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D019881:Aminoacyltransferases; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics; D002439:Cefotaxime / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology; D002648:Child; D021821:Communicable Diseases, Emerging / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D006602:Hexosyltransferases; D006801:Humans; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics; D010403:Penicillin Resistance / Q000235:genetics*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D011008:Pneumococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D014481:United States / Q000453:epidemiology
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D002511:Cephalosporins; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; D019881:Aminoacyltransferases; C478701:penicillin-binding protein 2b, Streptococcus; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D002439:Cefotaxime