Masses of phosphorylated neurofilaments are associated with abnormal golgi apparatus of anterior horn neurons of beta, beta'-iminodipropionitrile-intoxicated rats.
The Golgi apparatus (GA) of anterior horn neurons of rats chronically intoxicated with beta,beta'-iminodipropionitrile (IDPN) in drinking water was examined with an organelle-specific antibody. The neuropile of the anterior horns contained the typical axonal spheroids associated with IDPN toxicity while the perikarya of approximately one-third of the neurons contained phosphorylated neurofilaments, which are not found in the perikarya of control rat neurons. By serial or double immunostaining with the SMI-31 and anti-MG 160 antibodies, there were no morphological changes of the GA in the majority of neurons including neurons with a mild to moderate degree of neurofilamentous accumulation. However, a few neurons with a massive accumulation of phosphorylated neurofilaments contained abnormal profiles of the GA which consisted of focal clustering, reduction in size and fragmentation. The results suggest that masses of phosphorylated neurofilaments are associated with structural abnormalities of the GA.
Uesugi
Makoto
M
Department of Neurology, Gunma University School of Medicine, Maebashi, Japan.
Okamoto
Koichi
K
Tanaka
Makoto
M
Gonatas
Nicholas K
NK
D000818:Animals; D000870:Anterior Horn Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008297:Male; D016900:Neurofilament Proteins / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D019581:Neuropil / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D009570:Nitriles / Q000633:toxicity*; D009687:Nuclear Proteins / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor; D029701:Saccharomyces cerevisiae Proteins; D012795:Sialoglycoproteins / Q000378:metabolism; D014157:Transcription Factors
D016900:Neurofilament Proteins; D009570:Nitriles; D009687:Nuclear Proteins; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; C085451:SMI1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012795:Sialoglycoproteins; D014157:Transcription Factors; C086711:cysteine-rich fibroblast growth factor receptor; C008110:3,3'-iminodipropionitrile