title: Comparison of the expression patterns of two LIM-homeodomain genes, Lhx6 and L3/Lhx8, in the developing palate.
abstract: OBJECTIVE
  To compare and contrast the gene expression of two LIM-homeobox type transcription factors, Lhx6 and L3/Lhx8, in secondary palate formation.
  
  
  METHODS
  In situ hybridization histochemistry with digoxygenin (DIG) labelled cRNA probes specific for Lhx6 and L3/Lhx8.
  
  
  METHODS
  Serial cryo-sections of embryonic day (E)13.5, 14.5, and 15.5 mice (C57BL/6).
  
  
  METHODS
  Comparison of the signal intensities of NBT/BCIP precipitate by alkaline phosphatase conjugated anti-DIG antibody.
  
  
  RESULTS
  From E13.5 to E15.5, Lhx6 and L3/Lhx8 signals are detected in palatal mesenchyme, but the L3/Lhx8 signal is much more intense than the Lhx6 signal. In palatal epithelium, covering the mesenchyme, Lhx6 mRNA is transiently expressed at E14.5, while L3/Lhx8 mRNA expression is never detected throughout the development.
  
  
  CONCLUSIONS
  Lhx6 and L3/Lhx8 functions may be partially redundant in the mesenchyme of the secondary palate, but not in the palatal epithelium.
authors.affiliation: Department of Anesthesiology, School of Medicine, Fukushima Medical University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: H
authors.initials: H
authors.lastname: Takaki
authors.forename: M
authors.initials: M
authors.lastname: Takeuch
authors.forename: K
authors.initials: K
authors.lastname: Iseki
authors.forename: S
authors.initials: S
authors.lastname: Hagino
authors.forename: M
authors.initials: M
authors.lastname: Murakawa
authors.forename: S
authors.initials: S
authors.lastname: Yokoya
authors.forename: A
authors.initials: A
authors.lastname: Wanaka
mesh_terms: D000818:Animals; D002972:Cleft Palate / Q000196:embryology*; D005314:Embryonic and Fetal Development; D004848:Epithelium / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox / Q000502:physiology; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D060850:LIM-Homeodomain Proteins; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins; D021362:Palate, Hard / Q000196:embryology*; D015347:RNA Probes; D014157:Transcription Factors
chemical_list: D018398:Homeodomain Proteins; C498504:LHX6 protein, mouse; C112960:LIM homeobox protein 8; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D015347:RNA Probes; D014157:Transcription Factors