title: Microanatomical localization of PD-1 in human tonsils.
abstract: PD-1 is an immunoinhibitory receptor, which belongs structurally to the CD28 family. PD-1-deficient mice show breakdown of peripheral tolerance and manifest multiple autoimmune symptoms. We previously described expression of PD-1 on activated T and B lymphocytes and myeloid cells. However, little is known about the microanatomical distribution of PD-1 in lymphoid organs. In this study, we performed immunohistochemistry using monoclonal antibodies against human PD-1. In human tonsils, PD-1 was expressed on most of T cells and a small subset of centrocytes in the light zone of germinal centers (GCs), where clonal selection of centrocytes takes place. These results suggest that PD-1 may play an important role in GC reaction.
authors.affiliation: Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Iwai
authors.forename: Taku
authors.initials: T
authors.lastname: Okazaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nishimura
authors.forename: Akemi
authors.initials: A
authors.lastname: Kawasaki
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.forename: Tasuku
authors.initials: T
authors.lastname: Honjo
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface / Q000096:biosynthesis*; D051017:Apoptosis Regulatory Proteins; D001402:B-Lymphocytes / Q000378:metabolism; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D018858:Germinal Center / Q000166:cytology / Q000378:metabolism; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D051379:Mice; D014066:Palatine Tonsil / Q000166:cytology* / Q000378:metabolism; D061026:Programmed Cell Death 1 Receptor; D013601:T-Lymphocytes / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D051017:Apoptosis Regulatory Proteins; D019394:Ki-67 Antigen; C105992:PDCD1 protein, human; C491383:Pdcd1 protein, mouse; D061026:Programmed Cell Death 1 Receptor