title: [Expression of type I collagen and its receptor system in osteoblasts].
abstract: OBJECTIVE
  To study the expression of type I collagen and its receptor system-integrin alpha 2 beta 1 in different passages of osteoblasts.
  
  
  METHODS
  The expression of type I collagen and integrin alpha 2 beta 1 in the primary, sixth and fifteenth passage of osteoblasts were detected by S-P immunohistological staining technique, and their mRNA expression by quantity RT-PCR technique.
  
  
  RESULTS
  Type I collagen and integrin alpha 2 beta 1 were expressed in different passages of osteoblasts and there was no significant difference among three passages by immunohistological technique. Their mRNA expression was gradually decreased with subculture.
  
  
  CONCLUSIONS
  Type I collagen promotes the adhesion and phenotype expression of osteoblasts through its receptor-integrin alpha 2 beta 1. The reductive expression of type I collagen-receptor system will decline the phenotype of osteoblasts.
authors.affiliation: Department of Orthopedic Surgery, First University Hospital, West China University of Medical Sciences, Chengdu, Sichuan, P. R. China 610041.
authors.forename: X J
authors.initials: XJ
authors.lastname: Yu
authors.forename: Z M
authors.initials: ZM
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Qu
mesh_terms: D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D038982:Integrin alpha2beta1 / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D023822:Tissue Engineering
chemical_list: D024042:Collagen Type I; D038982:Integrin alpha2beta1; D012333:RNA, Messenger