title: Human bone marrow endothelial cells: a new identified source of B-type natriuretic peptide.
abstract: B-type natriuretic peptide (BNP) is a hormone mainly secreted by cardiac ventricle myocytes and which is increased in cardiac diseases. Moreover, BNP expression has been shown in various cell/tissue types. Six different human endothelial cell (EC) culture models arising from macro and microcirculation either primary cultures or cell lines were cultured and screened for BNP presence and secretion. All cell types expressed BNP mRNA while only the ECs arising from bone marrow stromal compartment secreted high amounts of BNP protein. This report is the first to identify ECs as a new source of BNP. However, BNP secretion is limited to a particular EC type.
authors.affiliation: U-443-INSERM, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, Bordeaux, France. laurence.bordenave@u-bordeaux2.fr
authors.forename: Laurence
authors.initials: L
authors.lastname: Bordenave
authors.forename: Agnès
authors.initials: A
authors.lastname: Georges
authors.forename: Reine
authors.initials: R
authors.lastname: Bareille
authors.forename: Véronique
authors.initials: V
authors.lastname: Conrad
authors.forename: Franck
authors.initials: F
authors.lastname: Villars
authors.forename: Joelle
authors.initials: J
authors.lastname: Amédée
mesh_terms: D009320:Atrial Natriuretic Factor / Q000032:analysis* / Q000235:genetics; D001854:Bone Marrow Cells / Q000737:chemistry*; D002460:Cell Line; D002478:Cells, Cultured; D004727:Endothelium / Q000737:chemistry* / Q000166:cytology*; D006801:Humans; D020097:Natriuretic Peptide, Brain; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011863:Radioimmunoassay; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor