Chemokine requirements for B cell entry to lymph nodes and Peyer's patches.
B cell entry to lymph nodes and Peyer's patches depends on chemokine receptor signaling, but the principal chemokine involved has not been defined. Here we show that the homing of CXCR4-/- B cells is suppressed in CCL19 (ELC)- and CCL21 (SLC)-deficient paucity of lymph node T cells mice, but not in wild-type mice. We also find that CXCR4 can contribute to T cell homing. Using intravital microscopy, we find that B cell adhesion to high endothelial venules (HEVs) is disrupted when CCR7 and CXCR4 are predesensitized. In Peyer's patches, B cell entry is dependent on CXCR5 in addition to CCR7/CXCR4. CXCL12 (SDF1) is displayed broadly on HEVs, whereas CXCL13 (BLC) is found selectively on Peyer's patch follicular HEVs. These findings establish the principal chemokine and chemokine receptor requirements for B cell entry to lymph nodes and Peyer's patches.
Okada
Takaharu
T
Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143, USA.
Ngo
Vu N
VN
Ekland
Eric H
EH
Förster
Reinhold
R
Lipp
Martin
M
Littman
Dan R
DR
Cyster
Jason G
JG
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002448:Cell Adhesion / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D054377:Chemokine CXCL12; D054382:Chemokine CXCL13; D018925:Chemokines / Q000378:metabolism*; D019743:Chemokines, CXC / Q000235:genetics / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology*; D012333:RNA, Messenger / Q000378:metabolism; D054400:Receptors, CCR7; D019718:Receptors, CXCR4 / Q000037:antagonists & inhibitors / Q000378:metabolism; D054380:Receptors, CXCR5; D019707:Receptors, Chemokine / Q000037:antagonists & inhibitors / Q000378:metabolism; D018121:Receptors, Cytokine / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D014699:Venules
C513179:CXCR5 protein, mouse; C515371:Ccr7 protein, mouse; D054377:Chemokine CXCL12; D054382:Chemokine CXCL13; D018925:Chemokines; D019743:Chemokines, CXC; C515256:Cxcl12 protein, mouse; C486386:Cxcl13 protein, mouse; D012333:RNA, Messenger; D054400:Receptors, CCR7; D019718:Receptors, CXCR4; D054380:Receptors, CXCR5; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D016201:Receptors, Lymphocyte Homing