Molecular cloning and characterization of oppo 1: a haploid germ cell-specific complementary DNA encoding sperm tail protein.
We isolated a cDNA clone specifically expressed during spermatogenesis from a subtracted cDNA library of mouse testis. The cDNA consisted of 1085 nucleotides and had an open reading frame of 870 nucleotides encoding a putative protein of 290 amino acid residues. Northern blot analysis revealed a 1.2-kilobase mRNA exclusively expressed in the testis in adult mice; the mRNA was first detected late pachytene stage, and expression increased as the animals matured. The protein encoded by the mRNA had a molecular weight of approximately 33 kDa by Western blot analysis, and was localized to occupy the flagella from the connecting piece through the principal piece. We named this newly isolated gene oppo 1, and we suggest that it plays an important role in sperm tail structure and/or sperm movement.
Nakamura
Yoshihiro
Y
Department of Urology, Osaka University Medical School, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Tanaka
Hiromitsu
H
Koga
Minoru
M
Miyagawa
Yasushi
Y
Iguchi
Naoko
N
Egydio de Carvalho
Carlos
C
Yomogida
Kentaro
K
Nozaki
Masami
M
Nojima
Hiroshi
H
Matsumiya
Kiyomi
K
Okuyama
Akihiko
A
Nishimune
Yoshitake
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D015723:Gene Library; D006238:Haploidy; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D029589:Seminal Plasma Proteins / Q000235:genetics; D013081:Sperm Motility / Q000235:genetics; D013082:Sperm Tail / Q000378:metabolism*; D013347:Subcellular Fractions / Q000737:chemistry; D013737:Testis / Q000737:chemistry
D012333:RNA, Messenger; D029589:Seminal Plasma Proteins; C470246:oppo 1 protein, mouse