title: Histological changes in canine aorta 1 year after stent-graft implantation: implications for the long-term stability of device anchoring zones.
abstract: OBJECTIVE
  To examine aortic histology 1 year after stent-graft implantation in a canine model as a means of assessing the durability of endograft fixation.
  
  
  METHODS
  Fourteen mongrel dogs each received 1 stent-graft and 1 bare stent placed endoluminally in the abdominal aorta. Eight animals were followed for 1 year, 3 for 4 to 8 weeks, and the remaining 3 for 24 to 48 hours. Aortic specimens were stained with hematoxylin-eosin, elastica-van Gieson, and Masson's trichrome and examined with light and electron microscopy and immunohistochemistry to identify smooth muscle cells (SMC), endothelialization, aortic wall ultrastructure, and changes at the device anchoring sites.
  
  
  RESULTS
  No dilatation or dissection was noted at any of the device anchoring sites. The aortic media at 1 year was remarkably decreased in thickness: 891 +/- 196 microm in the control tissue versus 388 +/- 70 microm for the proximal stent-grafted aorta and 457 +/- 148 microm for the bare-stented aortic segment. Other important histological features were reduced elastic lamellae in the stent-grafted aorta versus control (p<0.0001), increased SMC density in the stent-grafted aortic region (p<0.0001 versus control), and absence of inflammatory infiltrate. Complete neointimal covering and endothelialization of the luminal endograft surface were found. SMCs generally showed no ultrastructural features of necrosis.
  
  
  CONCLUSIONS
  Aortic stent-grafts induce distinctive histological changes in the aortic wall at 1 year, even when implanted in a healthy aorta. Although there is considerable medial elastin loss, an increased medial SMC density, an exuberant neointima, and a general absence of perigraft inflammation suggest an ongoing process of structural restoration at the device anchoring sites.
authors.affiliation: First Department of Surgery, Hamamatsu University School of Medicine, Handayama 1-20-1, Hamamatsu City 431-3192, Japan. basharahm@yahoo.com
authors.forename: Abul Hasan Muhammad
authors.initials: AH
authors.lastname: Bashar
authors.forename: Teruhisa
authors.initials: T
authors.lastname: Kazui
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Terada
authors.forename: Kazuya
authors.initials: K
authors.lastname: Suzuki
authors.forename: Naoki
authors.initials: N
authors.lastname: Washiyama
authors.forename: Katsushi
authors.initials: K
authors.lastname: Yamashita
authors.forename: Satoshi
authors.initials: S
authors.lastname: Baba
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000473:pathology* / Q000601:surgery; D019917:Blood Vessel Prosthesis Implantation; D004285:Dogs; D004730:Endothelium, Vascular / Q000473:pathology; D007150:Immunohistochemistry; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D015607:Stents; D013997:Time Factors; D017539:Tunica Intima / Q000473:pathology; D017540:Tunica Media / Q000473:pathology