title: Detection limit enhancement of fluorescent heterogeneities in turbid media by dual-interfering excitation.
abstract: We report on a quantitative comparison between the single-source and the dual-interfering-source configurations for the detection of fluorescent heterogeneities embedded in a piecewise highly scattering homogeneous fluorescent background. The study is based on simulations with analytical solutions of the frequency-domain fluorescent diffuse photon density waves and practical signal-to-noise ratio considerations. Results show that dual-interfering sources outperform single-source techniques for the detection of heterogeneities in terms of fluorophore concentration and lifetime contrast. To detect the same inhomogeneity, less concentration and lifetime contrast is required with dual-interfering sources.
authors.affiliation: Department of Biophysics and Biochemistry, University of Pennsylvania, Philadelphia 19104, USA. intes@mail.med.upenn.edu
authors.forename: Xavier
authors.initials: X
authors.lastname: Intes
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Xingde
authors.initials: X
authors.lastname: Li
authors.forename: Britton
authors.initials: B
authors.lastname: Chance
mesh_terms: D004396:Coloring Agents; D003198:Computer Simulation; D005453:Fluorescence; D006801:Humans; D007089:Image Enhancement; D007208:Indocyanine Green; D008962:Models, Theoretical; D017785:Photons; D012542:Scattering, Radiation; D019265:Spectroscopy, Near-Infrared
chemical_list: D004396:Coloring Agents; D007208:Indocyanine Green