title: Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system.
abstract: One hundred BacT/ALERT blood culture bottles growing gram-positive cocci in clusters were cultured and studied by LightCycler PCR for the sa442 and mecA genes. PCR was 100% sensitive and specific for detecting Staphylococcus aureus and methicillin resistance in S. aureus but was less accurate for methicillin resistance in coagulase-negative staphylococci.
authors.affiliation: Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
authors.forename: Nabin K
authors.initials: NK
authors.lastname: Shrestha
authors.forename: Marion J
authors.initials: MJ
authors.lastname: Tuohy
authors.forename: Gerri S
authors.initials: GS
authors.lastname: Hall
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Isada
authors.forename: Gary W
authors.initials: GW
authors.lastname: Procop
mesh_terms: D016470:Bacteremia / Q000175:diagnosis; D001426:Bacterial Proteins; D001431:Bacteriological Techniques / Q000379:methods / Q000706:statistics & numerical data; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005798:Genes, Bacterial; D006602:Hexosyltransferases; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase