In vitro radiosensitivity measured in lymphocytes and fibroblasts by colony formation and comet assay: comparison with clinical acute reactions to radiotherapy in breast cancer patients.
OBJECTIVE
To compare colony-forming and comet assays on fibroblasts and lymphocytes of 32 breast cancer patients irradiated after breast-conserving operations and to correlate the results with acute clinical radiation reactions in the skin.


METHODS
Skin fibroblasts were isolated and cultivated before radiotherapy and lymphocytes were drawn prior to the first and directly after the final external irradiation. The colony-forming assay was performed with fibroblasts and the comet assay with lymphocytes and fibroblasts of breast cancer patients according to standard protocols. The clinical radiation reactions of the patients were graded according to the RTOG system.


RESULTS
No significant correlation (p =0.09) was detected between clinical acute skin reactions and the in vitro clonogenic data in fibroblasts. Results of the comet assay in lymphocytes, however, showed a significant correlation (p <0.05) with the clinical data when patients were divided into two groups with average and elevated acute reactions. Apart from initial damage, fibroblasts did not show significant differences between the two patient groups. Repeated comet assays in lymphocytes of the same patient drawn before treatment and before and after external radiotherapy demonstrated good reproducibility of the test and no significant impact of preceding radiation treatment. There was a good correlation (r =0.65) between the comet assay results in fibroblasts and lymphocytes of the same individual.


CONCLUSIONS
In this cohort of patients, a significant correlation between the in vitro results of the comet assay in lymphocytes and clinical acute reactions was detected. The results of the comet assay and of fibroblast colony formation did not correlate with in vitro radiosensitivity.
Oppitz
U
U
Department of Radiation Therapy, University of Würzburg, Josef-Schneider-Str. 11, 97080 Würzburg, Germany. oppitz@strahlentherapie.uni-wuerzburg.de
Schulte
S
S
Stopper
H
H
Baier
K
K
Müller
M
M
Wulf
J
J
Schakowski
R
R
Flentje
M
M
D001943:Breast Neoplasms / Q000532:radiotherapy*; D015331:Cohort Studies; D020552:Comet Assay; D005260:Female; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D011836:Radiation Tolerance