Analysis of T-cell repertoire and mixed chimaerism in a patient with aplastic anaemia after allogeneic bone marrow transplantation.
We analysed 26 T-cell receptor (TCR) beta chain subfamilies (VB) of a patient with aplastic anaemia (AA) who underwent allogeneic bone marrow transplantation (allo-BMT). The patient developed pancytopenia at d 80. The patient's T cells were skewed in 10 of 26 TCR-VB on d 83. These TCR-VB, especially VB15, which were almost entirely CD8-positive cells, were skewed throughout her clinical course. Chimaerism analysis of the CD8-positive cells indicated that they were of recipient origin. Therefore, some immune responses induced by the recipient CD8-positive T cells had an important role in pancytopenia in AA patients after allo-BMT.
Tsutsumi
Yutaka
Y
Department ofHaematology and Oncology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Tanaka
Junji
J
Sugita
Junichi
J
Kato
Naoko
N
Zhang
Li
L
Yonezumi
Masakatsu
M
Chiba
Kouji
K
Toyosima
Nobuyasu
N
Kondo
Takeshi
T
Ohta
Shuichi
S
Mori
Akio
A
Hasino
Satoshi
S
Asaka
Masahiro
M
Imamura
Masahiro
M
D000328:Adult; D000741:Anemia, Aplastic / Q000276:immunology* / Q000628:therapy*; D016026:Bone Marrow Transplantation; D019075:Electrophoresis, Capillary; D005260:Female; D019674:Genes, T-Cell Receptor beta; D006801:Humans; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous