Chimpanzee homologous of human Y specific STRs. A comparative study and a proposal for nomenclature.
Eleven Y specific microsatellites, previously studied in humans, were typed for fragment length and sequenced in chimpanzees (Pan troglodytes). The primers described by Ayub et al. (Nucleic Acids Res. 28, 2000, 2) for amplifying DYS434, DYS435, DYS436, DYS437, DYS438, DYS439 and those described by White et al. (Genomics, 57, 1999, 433) for GATA A10, A7.1, A7.2, C4, and H4, were used to amplify DNA samples from chimpanzees. Primers described for Y GATA A4 were found to amplify the same region as reported for DYS439. Moreover, the GATA A4 forward primer only matches the repeat flanking region in 14 of the 28bp, being responsible for a very weak amplification. Therefore, this system was not included in this study. The analysis of the repeat and sequence structure observed in chimpanzee and human Y chromosomes allowed evolutionary comparisons as well as the basis for improving Y STR nomenclature and therefore, a unified nomenclature for these novel STRs is proposed to the scientific community following ISFG recommendations.
Gusmão
Leonor
L
Instituto de Patologia e Imunologia Molecular da Universidade do Porto (IPATIMUP), R. Dr. Roberto Frias, s/n, 4200 Porto, Portugal.
González-Neira
Annabel
A
Alves
Cíntia
C
Lareu
Maviky
M
Costa
Solange
S
Amorim
António
A
Carracedo
Angel
A
D000818:Animals; D006801:Humans; D008297:Male; D002679:Pan troglodytes / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017421:Sequence Analysis; D013045:Species Specificity; D020080:Tandem Repeat Sequences / Q000235:genetics*; D009626:Terminology as Topic; D014998:Y Chromosome / Q000235:genetics*