Age-related changes in nuclear receptor coactivator immunoreactivity in motoneurons of the spinal nucleus of the bulbocavernosus of male rats.
Immunoreactivity of phosphorylated cAMP response element binding protein-binding protein/p300 (CBP/p300) and steroid receptor coactivator-1 (SRC-1) was examined in motoneurons of the spinal nucleus of the bulbocavernosus (SNB) in young and old male rats by immunohistochemistry. In young animals, intense immunoreactive CBP/p300 and SRC-1 proteins were confined to the nuclei, but not in the nucleolus of SNB motoneurons. In old animals, both the intensity of CBP/p300 and SRC-1 immunoreactivity in the nuclei and number of CBP/p300 and SRC-1 immunoreactive nuclei of the SNB motoneurons were significantly reduced. A marked decline in the expression of CBP/p300 and SRC-1 in the aged SNB motoneurons suggests down-regulation of androgen receptor coactivator-mediated gene expression in the SNB system with advancing age.
Matsumoto
Akira
A
Department of Anatomy, Juntendo University School of Medicine, Hongo, Tokyo 113-0033, Japan. akira@med.juntendo.ac.jp
D000375:Aging / Q000378:metabolism*; D000818:Animals; D003307:Copulation / Q000502:physiology; D015536:Down-Regulation / Q000502:physiology; D050881:E1A-Associated p300 Protein; D004525:Efferent Pathways / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology; D051548:Histone Acetyltransferases; D007150:Immunohistochemistry; D008297:Male; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000294:innervation* / Q000502:physiology; D009687:Nuclear Proteins / Q000378:metabolism*; D056920:Nuclear Receptor Coactivator 1; D010413:Penis / Q000294:innervation / Q000502:physiology; D010502:Perineum / Q000294:innervation* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
D009687:Nuclear Proteins; D011944:Receptors, Androgen; D015534:Trans-Activators; D014157:Transcription Factors; D013739:Testosterone; D050881:E1A-Associated p300 Protein; C494362:Ep300 protein, rat; D051548:Histone Acetyltransferases; D056920:Nuclear Receptor Coactivator 1