Legume grains enhance ileal losses of specific endogenous serine-protease proteins in weaned pigs.
Feeding legume grains to pigs usually increases losses of endogenous proteins at the terminal ileum. However, the identity of such proteins is largely unknown. This study was undertaken to determine the ileal flow and identity of soluble proteins present in large concentrations in ileal digesta of young pigs fed soybean meal (SBM), peas (P), faba beans (FB), or blue lupin (L) in expt. 1, and white (WPC) or black (BPC) chickpeas in expt. 2. Protein in the control diet (C) was provided by casein. Ileal digesta proteins were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis, Coomassie blue staining, densitometry and N-terminal amino acid sequencing. Three protein bands at molecular masses of 25, 27, and 30 kDa had a higher ileal flow (P < 0.05) in the pigs fed the legume-based diets compared to those fed the control diet in expt. 2. This was true for the 25- and 30-kDa proteins (P < 0.05) and the 27-kDa protein (P < 0.10) in pigs fed the legume-containing diets in expt. 1. These proteins shared N-terminal amino acid sequences with enzymes of the serine protease family including pig trypsin (25 kDa) and blood coagulation factor IX or chymotrypsin (27 and 30 kDa).
Salgado
Paulo
P
Unité Mixte de Recherche sur le Veau et le Porc, INRA-ENSA de Rennes, 35042 Rennes Cedex, France.
Montagne
Lucile
L
Freire
João P B
JP
Ferreira
Ricardo B
RB
Teixeira
Artur
A
Bento
Ofélia
O
Abreu
Manuel C
MC
Toullec
René
R
Lallès
Jean-Paul
JP
D000595:Amino Acid Sequence / Q000235:genetics; D000596:Amino Acids / Q000032:analysis / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D015153:Blotting, Western; D001835:Body Weight; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae; D005766:Gastrointestinal Contents / Q000737:chemistry; D005772:Gastrointestinal Transit; D007082:Ileum / Q000378:metabolism*; D008297:Male; D010940:Plant Proteins / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism*; D012995:Solubility; D013552:Swine; D014886:Weaning
D000596:Amino Acids; D010940:Plant Proteins; D012697:Serine Endopeptidases