Quantitative stability of DNA after extended storage of clinical specimens as determined by real-time PCR.
Viral DNA stored for extended periods can be amplified by PCR. However, it is unknown whether stored specimens give accurate quantitative results by newer real-time PCR techniques. We therefore compared herpes simplex virus DNA levels in specimens before and after 16 months of storage. The levels of viral DNA remained stable whether the DNA was stored as purified DNA or unextracted DNA in a whole specimen.
Jerome
Keith R
KR
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA.
Huang
Meei-Li
ML
Wald
Anna
A
Selke
Stacy
S
Corey
Lawrence
L
D017088:AIDS-Related Opportunistic Infections / Q000821:virology; D000328:Adult; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics*; D004355:Drug Stability; D004356:Drug Storage; D005260:Female; D006558:Herpes Genitalis / Q000150:complications / Q000821:virology; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
D017931:DNA Primers; D004279:DNA, Viral