Characterization of minute differences between genomes of strains of Penicillium nalgiovense using subtractive suppression hybridization without cloning.
OBJECTIVE
Characterization of integrated DNA fragments of unknown base sequences from foreign species using the genetically modified mould Penicillium nalgiovense.


RESULTS
A modified suppression subtractive hybridization method was deployed in order to detect and characterize foreign genes. Combined with a special two-dimensional polyacrylamide gel electrophoretic technique the usual background was eliminated from the prospective foreign polymerase chain reaction fragments according to their base composition (Müller et al. 1981).
DNA fragments obtained from the procedure were suitable for direct DNA sequencing. Each DNA fragment derived from the tester genomic DNA was found to be completely absent in the driver organism.
Harms
C
C
University of Bremen, Centre of Environmental Research and Technology, UFT, Institute of Molecular Biology and Bioanalytics, Bremen, Germany. carsten@unin-bremen.de
Käse
M
M
Hildebrandt
A
A
D003001:Cloning, Molecular; D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis*; D016681:Genome, Fungal; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010407:Penicillium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity
D003470:Culture Media; D004271:DNA, Fungal