Cell-binding properties of glycoprotein B of Aujeszky's disease virus.
The glycoprotein B (gB) of Aujeszky's disease virus (ADV) has a role in virus entry and cell-to-cell spread. In this report we examined the cell-binding properties of native ADV gB purified from the virus envelope by affinity chromatography. The binding of gB to the surface of susceptible cells BHK-21 and MDBK was specific, dose-dependent, and nearly saturable, which is characteristic of conventional receptor-ligand interactions. The purified gB was shown to specifically bind to immobilised heparin. The addition of soluble exogenous heparin and heparinase treatment of cells inhibited the binding of gB to the cells. Cell-associated gB could also be dissociated from the cells by soluble heparin. The results indicated that ADV gB binds specifically to cellular heparan sulphate. The binding of gB to cells inhibited the attachment of virus to cells and thus the formation of viral plaques. The results suggest that ADV gB may have a function in the initial attachment of ADV to the surface of susceptible cells.
Vrublevskaya
Veronika V
VV
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.
Kornev
Aleksey N
AN
Smirnov
Sergey V
SV
Morenkov
Oleg S
OS
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002460:Cell Line; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism*; D011558:Herpesvirus 1, Suid / Q000737:chemistry*; D011485:Protein Binding; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
D019812:Heparan Sulfate Proteoglycans; D014759:Viral Envelope Proteins; C070915:glycoprotein gII, pseudorabies virus