[Cloning of a new cDNA: responded to all-trans retinoic acid in HL-60 cell differentiation].
In this paper a new type of cDNA fragment named as W-1 gene was first cloned and sequenced from ATRA induced HL-60 cells by using differential display PCR (DD-PCR) and DNA sequencing techniques. These differentially expressing products of the gene responding to ATRA were further confirmed by Northern blotting analysis. The results showed that the expressing level of this gene induced by ATRA (10(-6) mol.L-1) for 16 hrs was much higher than that in the control HL-60 cells, but its expression in HL-60 was reduced to an unestimable level after induction of ATRA (10(-6) mol.L-1) for 24 hrs. It suggests that ATRA may increase the expression of W-1 gene during the early stage of HL-60 cell differentiation. The exact mechanism of action is being studied further.
Wang
J
J
Research Center of Molecular Biology, Hunan Medical University, Changsha 410078.
Wang
S
S
Tian
Q
Q
Chen
H
H
Luo
Z
Z
Zeng
H
H
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018922:HL-60 Cells / Q000473:pathology; D006801:Humans; D008969:Molecular Sequence Data; D009857:Oncogenes; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology*
D000970:Antineoplastic Agents; D018076:DNA, Complementary; D012333:RNA, Messenger; D014212:Tretinoin