Discrimination of Arcobacter butzleri isolates by polymerase chain reaction-mediated DNA fingerprinting.
OBJECTIVE
The objective of this study was to subtype Arcobacter butzleri isolates using RAPD-PCR.


RESULTS
Thirty-five A. butzleri isolates obtained from chicken carcasses were examined. PCR-mediated DNA fingerprinting technique with primers of the variable sequence motifs was used to detect polymorphism within the isolates. Eleven distinct DNA profiles were obtained as follows: Of the 35 strains, 10 as profile 4; seven as profile 1; five as profile 3; three as profiles 2 and 9; two as profile 10; one as profiles 5, 6, 7, 8 and 11.


CONCLUSIONS
Chicken carcasses sold in markets were found to be contaminated with several different strains of A. butzleri. RAPD-PCR technique was found to be a useful technique for distinguishing A. butzleri isolates.


CONCLUSIONS
The presence of several different A. butzleri strains on chicken carcasses may indicate multiple sources of contamination. The epidemiological role of A. butzleri in human and animal diseases should be investigated further.
Atabay
Halil Ibrahim
HI
Department of Microbiology, Veterinary Faculty, Kafkas University, Kars, Turkey. hiatabay@hotmail.com
Bang
D D
DD
Aydin
F
F
Erdogan
H M
HM
Madsen
M
M
D000818:Animals; D020640:Arcobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D002645:Chickens / Q000382:microbiology*; D016172:DNA Fingerprinting; D017931:DNA Primers; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers