title: Glucidic determinants expressed by the digestive apparatus of Umbrina cirrosa (L.) fries as revealed by lectin histochemistry.
abstract: Glycoconjugates present in the various parts of the digestive apparatus (oesophagus, stomach, intestine) of 27, 34 and 44 days-old Umbrina cirrosa (L.) fries were characterised by means of lectin histochemistry in conjunction with sialidase digestion and KOH treatment. High amounts of carbohydrates were detected especially in goblet cells of the oesophagus. Positivity obtained with the various lectins in oesophagus, stomach and intestine did not change during the development of the fries examined. D-Gal and D-GalNAc appeared to occupy a terminal position in oligosaccharidic chains whereas they are linked to sialic acid residues in adult subjects, which suggests an incomplete chemical structure of the glycoconjugates secreted by the digestive apparatus of fries.
authors.affiliation: Department of Biopathological Veterinary Science, Faculty of Veterinary Medicine, Università degli Studi di Perugia, Italy. anatvet@unipg.it
authors.forename: Francesco
authors.initials: F
authors.lastname: Parillo
authors.forename: Oliviero
authors.initials: O
authors.lastname: Fagioli
authors.forename: Piero
authors.initials: P
authors.lastname: Ceccarelli
mesh_terms: D000818:Animals; D004396:Coloring Agents; D004064:Digestive System / Q000378:metabolism*; D004947:Esophagus / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D006027:Glycosides / Q000096:biosynthesis*; D006651:Histocytochemistry; D006878:Hydroxides; D007413:Intestinal Mucosa / Q000378:metabolism; D037102:Lectins; D009439:Neuraminidase / Q000737:chemistry; D009844:Oligosaccharides / Q000378:metabolism; D010473:Perciformes / Q000502:physiology*; D010544:Peroxidases; D017680:Potassium Compounds; D013194:Staining and Labeling
chemical_list: D004396:Coloring Agents; D006027:Glycosides; D006878:Hydroxides; D037102:Lectins; D009844:Oligosaccharides; D017680:Potassium Compounds; D010544:Peroxidases; D009439:Neuraminidase; C029943:potassium hydroxide