The effects of ethanol on glucose 6-phosphate dehydrogenase enzyme activity from human erythrocytes in vitro and rat erythrocytes in vivo.
OBJECTIVE
The effects of ethanol on erythrocyte glucose 6-phosphate dehydrogenase (G6PD) activity were investigated under in vitro and in vivo conditions.


METHODS
For in vitro studies, glucose 6-phosphate dehydrogenase was purified from human erythrocyte and rats were used for in vivo studies. Enzyme activity was determined spectrophotometrically by the Beutler method.


RESULTS
The in vitro study showed that the I(50) value was 17 mM for ethanol. In the case of the in vivo study, a 2 ml/kg dose of ethanol significantly inhibited the G6PD activity. The inhibition rate after ethanol administration was 59%, 40% and 6% at 1, 3 and 6 h after, respectively.


CONCLUSIONS
The results of this study suggest that ethanol has a significant inhibitory effect on the G6PD activity both in vivo and in vitro.
Büyükokuroğlu
Mehmet Emin
ME
Departments of Pharmacology and Biochemistry, Medical Faculty and Biotechnology Application and Research Center, Atatürk University, Erzurum, Turkey.
Altikat
Sayit
S
Ciftçi
Mehmet
M
D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D000431:Ethanol / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000431:Ethanol; D005954:Glucosephosphate Dehydrogenase