Changes in nuclear matrix proteins during the senescence-like phenomenon induced by 5-chlorodeoxyuridine in HeLa cells.
5-Chlorodeoxyuridine (CldU) immediately induces a senescence-like phenomenon in any type of mammalian cells probably due to a change in nuclear matrix structure. We thus examined nuclear matrix proteins in HeLa cells cultured with CldU by high-resolution two-dimensional gel electrophoresis and peptide mass spectrometry. Three proteins were found to be down-regulated and one protein up-regulated by addition of CldU. In addition, one acidic protein accumulated in the nuclear matrix, although not quantitatively changed in the nuclei. Since these alterations were observed within 24h after addition of CldU, these proteins may be involved in an early step of the senescence-like phenomenon.
Michishita
Eriko
E
Division of Biochemistry, Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Maioka-cho 641-12, Japan.
Kurahashi
Toshihiro
T
Suzuki
Toshikazu
T
Fukuda
Masafumi
M
Fujii
Michihiko
M
Hirano
Hisashi
H
Ayusawa
Dai
D
D034961:Antigens, Nuclear; D016922:Cellular Senescence / Q000502:physiology*; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000032:analysis*
D034961:Antigens, Nuclear; D009687:Nuclear Proteins; C012244:5-chloro-2'-deoxyuridine; D003857:Deoxyuridine