Frequent mutations of Fas gene in nasal NK/T cell lymphoma.
Fas (Apo-1/CD95) is a cell-surface receptor involved in cell death signaling through binding of Fas ligand. Mutation of Fas gene in lymphoid cells results in accumulation of these cells, which might thus contribute to lymphomagenesis. We examined the open reading frame of Fas cDNA in 14 cases of nasal NK/T-cell lymphoma. Mutations of Fas gene were detected in seven (50%) of 14 cases which comprised four frameshift, two missense, and one silent mutations. Frameshift mutations were caused by insertion of 1 bp (A) at nucleotide 1095 in two cases and by deletion of 1 bp at nucleotide 597 and at 704, respectively, in one each. Mouse T-cell lymphoma cells transfected with two missense mutated genes and frameshift mutations caused by insertion of 1 bp (A) at nucleotide 1095 were resistant to apoptosis induced by the anti-Fas antibody. These findings suggested that accumulation of lymphoid cells with Fas mutations provides a basis for the development of nasal NK/T-cell lymphoma.
Takakuwa
Tetsuya
T
Department of Pathology, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.
Dong
Zhiming
Z
Nakatsuka
Shinichi
S
Kojya
Shizuo
S
Harabuchi
Yasuaki
Y
Yang
Woo-Ick
WI
Nagata
Shigekazu
S
Aozasa
Katsuyuki
K
D017209:Apoptosis / Q000235:genetics; D004252:DNA Mutational Analysis; D005434:Flow Cytometry; D020022:Genetic Predisposition to Disease; D006103:Granuloma, Lethal Midline / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007694:Killer Cells, Natural / Q000473:pathology*; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000473:pathology; D009154:Mutation / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019014:fas Receptor / Q000235:genetics*
D012333:RNA, Messenger; D019014:fas Receptor