An improved method for recovery of F-defective Sendai virus expressing foreign genes from cloned cDNA.
An improved system is described to recover non-transmissible Sendai virus that lack the envelope fusion (F) gene from cloned cDNA. The system (1) used plasmids that expressed the F and the HN viral envelope proteins, as well as the plasmids that expressed the viral NP, P, and L proteins as helper plasmids for recovery, and (2) overlaid packaging cells that expressed the F protein. With this improved system, we have succeeded in recovery of F-defective Sendai virus expressing two foreign proteins, and expression vectors that do not contain the EGFP reporter gene. This system may provide the basis for constructing recombinant F-defective Sendai virus for preventing and treating human diseases in the form of vaccines and vectors for gene therapy.
Hirata
Takahiro
T
DNAVEC Research Inc., 1-25-11 Kannondai, Tsukuba-shi, Ibaraki 305-0856, Japan.
Iida
Akihiro
A
Shiraki-Iida
Takako
T
Kitazato
Kaio
K
Kato
Atsushi
A
Nagai
Yoshiyuki
Y
Hasegawa
Mamoru
M
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral / Q000235:genetics; D003673:Defective Viruses / Q000235:genetics*; D015686:Gene Products, env / Q000235:genetics; D016679:Genome, Viral; D008253:Macaca mulatta; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D029082:Sendai virus / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000235:genetics
D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral; D015686:Gene Products, env; D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins