Monitoring and control of pullulan production using vision sensor.
The production of the polysaccharide pullulan by the yeast-like fungi, Aureobasidium pullulans, is accompained by cellular morphogenetic changes. High productivity and yield of the process have been found to correlate with high concentration of yeast-like cells in the culture. The morphogenetic changes of A. pullulans cells depend on the culture conditions, e.g., dissolved oxygen, shear rate and medium composition. In order to improve the productivity of the process, a novel control law was formulated. A feeding strategy dependent on the culture cellular composition was designed and aimed to keep the yeast-like cell concentration high. The culture morphogenetic composition during the process was monitored by a recently developed vision sensor. Feeding was actuated when the yeast-like cell concentration decreased below a threshold. The proposed control strategy improved pullulan production by increasing both productivity and yield of the cells by 67% and 80%, correspondingly. The results point to the advantage and the potential of using the monitoring and control system and algorithm to increase productivity and yield in cellular bioprocesses.
Ronen
M
M
Unit of Biotechnology, Faculty of Engineering Sciences, Ben Gurion University of the Negev, P.O. Box 653, 84105, Beer-Sheva, Israel. michal.ronen@weizmann.ac.il
Guterman
H
H
Shabtai
Y
Y
D001203:Ascomycota / Q000254:growth & development*; D018533:Biomass; D005936:Glucans / Q000096:biosynthesis*; D007700:Kinetics; D009172:Mycology / Q000379:methods; D013997:Time Factors
D005936:Glucans; C009109:pullulan