title: Attenuation of glucan-binding protein C reduces the cariogenicity of Streptococcus mutans: analysis of strains isolated from human blood.
abstract: A blood isolate of Streptococcus mutans strain TW871 shows relatively low homology with MT8148, a reference oral isolate strain, and lacks the serotype-specific polysaccharide antigen, suggesting that other cell-surface structures correlate with cariogenicity. We compared cariogenicity of TW871 with MT8148 (serotype c) and blood isolate TW964 (serotype f) in rats. Strain TW871 showed significantly lower cariogenicity than MT8148 or TW964 and expressed significantly lower sucrose-independent cellular adhesion to saliva-coated hydroxyapatite and dextran-binding activity than strain MT8148. Strains TW871 and TW964 showed a defect in the gbpA gene by Southern hybridization analysis, while sequencing analysis revealed gbpC variation in TW871. These results suggest that variation in GbpC may alter cellular adherence properties and can be correlated with the cariogenicity of S. mutans in this strain.
authors.affiliation: Department of Pedodontics, Osaka University Graduate School of Dentistry, 1-8 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakano
authors.forename: M
authors.initials: M
authors.lastname: Matsumura
authors.forename: M
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
authors.forename: S
authors.initials: S
authors.lastname: Sobue
authors.forename: I
authors.initials: I
authors.lastname: Nakagawa
authors.forename: S
authors.initials: S
authors.lastname: Hamada
authors.forename: T
authors.initials: T
authors.lastname: Ooshima
mesh_terms: D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D016470:Bacteremia / Q000097:blood; D001422:Bacterial Adhesion / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D015139:Blotting, Southern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics* / Q000502:physiology; D002648:Child; D003731:Dental Caries / Q000382:microbiology*; D017886:Durapatite / Q000378:metabolism; D004697:Endocarditis, Bacterial / Q000097:blood; D014644:Genetic Variation; D005936:Glucans / Q000378:metabolism*; D006801:Humans; D037102:Lectins; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D051381:Rats; D017207:Rats, Sprague-Dawley; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D013295:Streptococcus mutans / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D005936:Glucans; D037102:Lectins; C037937:glucan-binding proteins; D017886:Durapatite