RelA protein is involved in induction of genetic competence in certain Bacillus subtilis strains by moderating the level of intracellular GTP.
We found that the ability to develop genetic competence of a certain relaxed (relA) aspartate-auxotrophic strain of Bacillus subtilis is significantly lower than that of the isogenic stringent (relA+) strain. Transcriptional fusion analysis utilizing a lacZ reporter gene indicated that the amount of the ComK protein, known as the key protein for competence development, is greatly reduced in the relaxed strain than in the stringent strain. We also found that the addition of decoyinine, a GMP synthetase inhibitor, induces expression of a competence gene (comG) in the relaxed strain, accompanied by a pronounced decrease in the level of intracellular GTP as measured by high-performance liquid chromatography. The transformation efficiency of the relaxed strain increased 100-fold when decoyinine was added at t0 (the transition point between exponential to stationary growth phase). Conversely, supplementation of guanosine together with decoyinine completely abolished the observed effect of adding decoyinine on competence development. Furthermore, the impaired ability of the relaxed strain for competence development was completely restored by disrupting the codY gene, which is known to negatively control comK expression. Our results indicate that the RelA protein plays an essential role in the induction of competence development at least under certain physiological conditions by reducing the level of intracellular GTP and overcoming CodY-mediated regulation.
Inaoka
Takashi
T
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Ochi
Kozo
K
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D001224:Aspartic Acid / Q000494:pharmacology; D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001426:Bacterial Proteins / Q000502:physiology; D004268:DNA-Binding Proteins; D006157:Guanosine Monophosphate / Q000096:biosynthesis; D006160:Guanosine Triphosphate / Q000378:metabolism*; D008025:Ligases / Q000502:physiology*; D012097:Repressor Proteins / Q000502:physiology; D013171:Spores, Bacterial / Q000502:physiology
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C012182:angustmycin A; D001224:Aspartic Acid; D006157:Guanosine Monophosphate; D006160:Guanosine Triphosphate; D008025:Ligases; C028812:guanosine 3',5'-polyphosphate synthetases; D000241:Adenosine