Characterisation of the QM gene of Trypanosoma brucei.
The QM protein has been reported to have roles in both tumour suppression and transcription factor regulation in vertebrate cells, and in ribosome stability in both yeast and mammals. The present study isolated the QM gene of Trypanosoma brucei and determined its sequence. Alignment with QM sequences from Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster and Homo sapiens revealed greater than 60% identity. Southern blot analysis revealed multiple copies of QM within the trypanosome genome. An epitope tag was inserted into the C-terminus of the T. brucei QM and the protein expressed under inducible control in procyclic form trypanosomes. Immune fluorescence microscopy revealed co-localisation with the GPI:protein transamidase component, GPI8, a distribution indicative of ribosome association with the rough endoplasmic reticulum.
Lillico
Simon G
SG
Wellcome Centre for Molecular Parasitology, University of Glasgow, The Anderson College, Glasgow G11 6NU, UK.
Mottram
Jeremy C
JC
Murphy
Noel B
NB
Welburn
Susan C
SC
D000595:Amino Acid Sequence; D000818:Animals; D029681:Arabidopsis Proteins; D002352:Carrier Proteins / Q000235:genetics; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics*; D000080882:Ribosomal Protein L10; D012269:Ribosomal Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000235:genetics*
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D015800:Protozoan Proteins; C465198:QM protein, Trypanosoma brucei; D012269:Ribosomal Proteins; D014157:Transcription Factors; C000630691:ribosomal protein L10, Arabidopsis