title: Mupirocin resistance in methicillin-resistant Staphylococcus aureus clinical isolates in a Spanish hospital. Co-application of multiplex PCR assay and conventional microbiology methods.
abstract: A total of 1785 Staphylococcus aureus clinical isolates were collected in our hospital during 1998 (526), 1999 (564) and 2000 (695). Among them, one hundred and thirty (39, 33 and 58, respectively) were phenotypically assigned as methicillin-resistant Staphylococcus aureus (MRSA); sixteen of these isolates (3, 2 and 11, respectively) were detected as highly mupirocin-methicillin resistant Staphylococcus aureus (MMRSA). In this work, our goal was to characterize MRSA and MMRSA clinical isolates by co-application of phenotypic and genotypic methods in order to determine the MMRSA incidence in our hospital during the period 1998-2000. With this purpose we compared and integrated the results obtained using conventional microbiology methods with those obtained using a multiplex polymerase chain reaction (PCR) assay. Our results showed a good complementation between these two approximations to determine the incidence of MMRSA clinical isolates and permitted to estimate that such incidence increased from 7.7% in 1998 to 19% in 2000.
authors.affiliation: Molecular Biology Laboratory, Research Unit, Nuestra Señora de Candelaria University Hospital, Santa Cruz de Tenerife, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Pérez-Roth
authors.forename: F
authors.initials: F
authors.lastname: Claverie-Martín
authors.forename: N
authors.initials: N
authors.lastname: Batista
authors.forename: A
authors.initials: A
authors.lastname: Moreno
authors.forename: S
authors.initials: S
authors.lastname: Méndez-Alvarez
mesh_terms: D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006602:Hexosyltransferases; D006761:Hospitals; D006801:Humans; D016106:Methicillin Resistance; D016712:Mupirocin / Q000494:pharmacology*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D010641:Phenotype; D016133:Polymerase Chain Reaction; D013030:Spain; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D005819:Genetic Markers; D046915:Penicillin-Binding Proteins; D016712:Mupirocin; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase