[The study of new bio-membrane-bred epidermal cell grafts].
OBJECTIVE
The human epidermal cells were bred on a kind of bio-membrane, the bio-brane, in engineering a kind of new epidermal substitute, the bio-membrane bred cell graft.


METHODS
Fresh and frozen grafts of biomembrane bred epidermal cells were transplanted into the full-thickness wounds of nude mice and those received simple Bio-brane were served as control. The wounds of the two groups were observed daily and biopsy was taken on the 3, 5, 7, 10, 21 and 35 days respectively.


RESULTS
Epidermal cells could be cultured in vitro on the bio-membrane reaching the sub-saturated state of 60 to 70 percents. The bio-membrane after being grafted the epidermal cells continued to proliferate and differentiate to form a layer of new epidermis. There was no difference between the fresh and the frozen bio-membranes.


CONCLUSIONS
Bio-membrane bred with epidermal cells could be a kind of ideal epidermal substitute.
Jiao
X Y
XY
Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, P. R. China 200443. jscao@ecmu.org.cn
Kopp
J
J
Xing
X
X
D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D000078404:Epidermal Cells; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D008819:Mice, Nude; D014945:Wound Healing; D014947:Wounds and Injuries / Q000601:surgery*
D008567:Membranes, Artificial