Acute 3rd-ventricular amylin infusion potently reduces food intake but does not produce aversive consequences.
In this study, a conditioned taste aversion (CTA) paradigm was used to assess the possibility that 3rd-ventricular (i3vt) administration of the pancreatic hormone amylin produces aversive consequences that secondarily reduce food intake independently of the normal regulation of energy balance. After 1-h daily access to water for 7 days, rats were given 1-h access to a 0.15% saccharin solution, followed immediately by i3vt amylin (100 pmol) in one group (n=7) and i3vt CSF vehicle in another (n=7). As positive control for the formation of a CTA, a third group of seven rats received intraperitonial (i.p.) lithium chloride (LiCl). Saline was given i.p. to a fourth group (n=7) as control for i.p. LiCl. As expected, the LiCl rats exhibited a marked aversion to the saccharin in a subsequent two-bottle intake test. In contrast, although the 100 pmol i3vt amylin dose is substantially higher than that required to reduce food intake, no evidence of a CTA was observed in the rats that had received i3vt amylin. In summary, these data are consistent with the conclusion that acute i3vt amylin infusion does not reduce food intake by producing aversive consequences.
Rushing
Paul A
PA
Department of Psychiatry, College of Medicine, University of Cincinnati, Cincinnati, OH 45267-0559, USA.
Seeley
Randy J
RJ
Air
Ellen L
EL
Lutz
Thomas A
TA
Woods
Stephen C
SC
D000682:Amyloid / Q000008:administration & dosage* / Q000009:adverse effects / Q000494:pharmacology*; D000818:Animals; D001067:Appetite Depressants / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology; D003213:Conditioning, Psychological; D004734:Energy Metabolism; D005247:Feeding Behavior / Q000187:drug effects* / Q000523:psychology; D007274:Injections, Intraperitoneal; D058228:Islet Amyloid Polypeptide; D018021:Lithium Chloride / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D051381:Rats; D020318:Rats, Long-Evans; D012439:Saccharin / Q000008:administration & dosage / Q000494:pharmacology; D013649:Taste / Q000502:physiology; D013997:Time Factors
D000682:Amyloid; D001067:Appetite Depressants; D058228:Islet Amyloid Polypeptide; D012439:Saccharin; D018021:Lithium Chloride