The influence of repair tissue maturation on the response to oscillatory compression in a cartilage defect repair model.
This study examined the effects of mechanical compression on engineered cartilage in a novel hybrid culture system. Cylindrical holes were cut in discs of bovine articular cartilage and filled with agarose gels containing chondrocytes. These constructs were compressed in radiolabeled medium under static or oscillatory unconfined compression. Oscillatory compression at 1 Hz significantly stimulated synthesis above static control levels. Control experiments indicate that oscillatory compression does not stimulate freshly cast gels (without annuli), but does so after several weeks. This may be because physiologic fluid flow levels do not occur until sufficient extracellular matrix has accumulated. Finite element models predict minimal fluid flow in the gel core, and minimal differences in flow patterns between free and constrained gels. However, the models predict fluid pressures in constrained gels to be substantially higher than those in free gels. Our results suggest that pressure variations may influence synthesis of engineered cartilage matrices, with implications for construct development and post-implantation survival.
Hunter
Christopher J
CJ
Georgia Institute of Technology, Wallace H. Coulter Department of Biomedical Engineering, Atlanta, GA, USA.
Levenston
Marc E
ME
D000704:Analysis of Variance; D000818:Animals; D002358:Cartilage, Articular / Q000293:injuries* / Q000502:physiology*; D002417:Cattle; D008954:Models, Biological; D012038:Regeneration; D012212:Rheology; D013314:Stress, Mechanical; D023822:Tissue Engineering