Anaerostipes caccae gen. nov., sp. nov., a new saccharolytic, acetate-utilising, butyrate-producing bacterium from human faeces.
Two strains of a previously undescribed Eubacterium-like bacterium were isolated from human faeces. The strains are Gram-variable, obligately anaerobic, catalase negative, asporogenous rod-shaped cells which produced acetate, butyrate and lactate as the end products of glucose metabolism. The two isolates displayed 99.9% 16S rRNA gene sequence similarity to each other and treeing analysis demonstrated the faecal isolates are far removed from Eubacterium sensu stricto and that they represent a new subline within the Clostridium coccoides group of organisms. Based on phenotypic and phylogenetic criteria, it is proposed that the two strains from faeces be classified as a new genus and species, Anaerostipes caccae. The type strain of Anaerostipes caccae is NCIMB 13811T (= DSM 14662T).
Schwiertz
Andreas
A
Department of Gastrointestinal Microbiology, German Institute of Human Nutrition, Bergholz-Rehbrücke. andy@www.dife.de
Hold
Georgina L
GL
Duncan
Sylvia H
SH
Gruhl
Barbel
B
Collins
Matthew D
MD
Lawson
Paul A
PA
Flint
Harry J
HJ
Blaut
Michael
M
D000085:Acetates / Q000378:metabolism; D001483:Base Sequence; D020148:Butyric Acid / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D006093:Gram-Positive Asporogenous Rods / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D013045:Species Specificity; D013395:Sucrose / Q000378:metabolism
D000085:Acetates; D012336:RNA, Ribosomal, 16S; D020148:Butyric Acid; D013395:Sucrose