Spectrophotometric determination of etidocaine in pharmaceutical (dental) formulation.
A spectrophotometric method was developed for the determination of etidocaine hydrochloride (EH) in injectable pharmaceutical preparation. The proposal of this work was to develop a rapid, simple, inexpensive, precise and accurate visible spectrophotometric method. The method is based on the formation of the ion-pair complex by the EH reaction with bromocresol green in the pH 4.6 which after chloroform extraction gives a yellow color that in basic medium change to blue color and exhibits a maximum absorbance at 625 nm. The calibration graph was linear over the range 2.0-6.0 microg ml(-1) EH calculated on the final yellow solution. The R.S.D. of the slope of the four lines was 0.73%. This method can be applied to injectable pharmaceutical preparation dosage studied.
Silva
Nelson
N
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, Porto Alegre, R/S, CEP 90160-000, Porto Alegre, Brazil. nieelson@hotmail.com
Schapoval
Elfrides E S
EE
D000766:Anesthesia, Dental; D000779:Anesthetics, Local / Q000032:analysis* / Q000737:chemistry; D001961:Bromcresol Green / Q000737:chemistry; D002021:Buffers; D005041:Etidocaine / Q000032:analysis* / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis*; D015203:Reproducibility of Results; D019346:Sodium Acetate; D013053:Spectrophotometry
D000779:Anesthetics, Local; D002021:Buffers; D004364:Pharmaceutical Preparations; D019346:Sodium Acetate; D001961:Bromcresol Green; D005041:Etidocaine