Molecular Design and Bio-activity Analysis of a t-PA Derivative (t-PA S165W).
In order to obtain tissue-type plasminogen activator (t-PA) mutant with enhanced affinity for fibrin, redesigning t-PA strategies were proposed by computer molecular designing technology, and the re-designed t-PA derivative, t-PA S165W, was expressed in CHO cells. The bio-activities and affinity for fibrin of the expression product were tested. No apparent changes were observed between t-PA S165W and wild type t-PA. Therefore, single site mutation of the t-PA could not lead to enhanced affinity for fibrin of t-PA.
Zhou
Hong
H
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. huangcf@nic.bmi.ac.cn
Wang
Guo-Li
GL
Huang
Pei-Tang
PT
Huang
Cui-Fen
CF