title: Crystallization and preliminary X-ray crystallographic studies on recombinant human carnitine acetyltransferase.
abstract: In this paper, the purification, crystallization and preliminary X-ray crystallographic studies of human carnitine acetyltransferase are reported. Recombinant human carnitine acetyltransferase crystals were grown by the hanging-drop vapor-diffusion method and belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 137.65, b = 84.76, c = 57.65 A and one molecule per asymmetric unit. The intensity data were collected from a cryocooled crystal to 1.6 A resolution using a conventional X-ray source.
authors.affiliation: Department of Medicinal Chemistry, McKnight Brain Institute and University of Florida, Gainesville 32610, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Lian
authors.forename: Lakshmanan
authors.initials: L
authors.lastname: Govindasamy
authors.forename: Yunrong
authors.initials: Y
authors.lastname: Gu
authors.forename: Thomas
authors.initials: T
authors.lastname: Kukar
authors.forename: Mavis
authors.initials: M
authors.lastname: Agbandje-McKenna
authors.forename: Robert
authors.initials: R
authors.lastname: McKenna
authors.forename: Donghai
authors.initials: D
authors.lastname: Wu
mesh_terms: D002332:Carnitine O-Acetyltransferase / Q000737:chemistry*; D018360:Crystallography, X-Ray / Q000379:methods*; D004058:Diffusion; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry*
chemical_list: D011994:Recombinant Proteins; D002332:Carnitine O-Acetyltransferase