title: Poly(L-lysine)-g-poly(D,L-lactic-co-glycolic acid) micelles for low cytotoxic biodegradable gene delivery carriers.
abstract: Poly(lactic-co-glycolic acid) (PLGA)-grafted poly(L-lysine) (PLL) (PLL-g-PLGA) was synthesized to demonstrate its micelle-forming property in an aqueous solution. The micelles were used as a gene delivery carrier. The hydrodynamic diameter of PLL-g-PLGA micelles in an aqueous solution was ca. 149 nm with a narrow size distribution. Critical micelle concentration (cmc) was 9.6 mg/l. The PLL-g-PLGA micelles could be used to produce compact nanoparticulate complexes with plasmid DNA, which could efficiently protect the complexed DNA from enzymatic degradation by DNase I. The micelle/DNA complexes had highly compacted structure sized between 200-300 nm with a positive surface charge value. The PLL-g-PLGA micelles exhibited much higher transfection efficiency with lower cytotoxicity than PLL. Here, we demonstrated that biodegradable and cationic PLL-g-PLGA micelles could be used as an effective DNA condensation carrier for gene delivery system.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Jeong
authors.forename: Tae Gwan
authors.initials: TG
authors.lastname: Park
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D018014:Gene Transfer Techniques; D008239:Lysine; D051379:Mice; D008823:Micelles; D010957:Plasmids / Q000737:chemistry; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection
chemical_list: D004337:Drug Carriers; D008823:Micelles; D011091:Polyesters; D011506:Proteins; C466993:poly(lactic-co-glycolic acid)-grafted-poly(L-lysine); D004247:DNA; D008239:Lysine