title: Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds.
abstract: A novel biodegradable hydroxyapatite/chitosan-gelatin network (HA/CS-Gel) composite of similar composition to that of normal human bone was prepared as a three-dimensional biomimetic scaffold by phase separation method for bone tissue engineering. Changing the solid content and the compositional variables of the original mixtures allowed control of the porosities and densities of the scaffolds. The HA granules were dispersed uniformly in the organic network with intimate interface contact via pulverizing and ultrasonically treating commercial available HA particles. Scaffolds of 90.6% porosity were used to examine the proliferation and functions of the cells in this three-dimensional microenvironment by culturing neonatal rat caldaria osteoblasts. Histological and immunohistochemical staining and scanning electron microscopy observation indicated that the osteoblasts attached to and proliferated on the scaffolds. Extracellular matrices including collagen I and proteoglycan-like substrate were synthesized, while osteoid and bone-like tissue formed during the culture period. Furthermore, the cell/scaffold constructs had good biomineralization effect after 3 weeks in culture.
authors.affiliation: Research Institute of Polymeric Materials, Tianjin University, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yin
authors.forename: William W
authors.initials: WW
authors.lastname: Lu
authors.forename: J Chiyan
authors.initials: JC
authors.lastname: Leong
authors.forename: Wenyi
authors.initials: W
authors.lastname: Zhang
authors.forename: Jingyu
authors.initials: J
authors.lastname: Zhang
authors.forename: Mingfang
authors.initials: M
authors.lastname: Zhang
authors.forename: Kangde
authors.initials: K
authors.lastname: Yao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000302:isolation & purification*; D001673:Biodegradation, Environmental; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D048271:Chitosan; D017886:Durapatite / Q000302:isolation & purification*; D005780:Gelatin / Q000302:isolation & purification*; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D051381:Rats; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D002686:Chitin; D005780:Gelatin; D048271:Chitosan; D017886:Durapatite