The co-expression of ASIC3 with calcitonin gene-related peptide and parvalbumin in the rat trigeminal ganglion.
The co-expression of ASIC3 with calcitonin gene-related peptide (CGRP) or parvalbumin (PV) was examined in the trigeminal ganglion (TG) by a double immunofluorescence method. ASIC3-immunoreactivity (IR) was detected in 23% of TG neurons. These neurons were of various sizes (range= 43-1768 microm(2), mean+/-S.D.=651+/-356 microm(2)); 26% and 14% of ASIC3-immunoreactive (IR) neurons co-expressed CGRP- and PV-immunoreactivity (IR), respectively; 33% and 13% of the TG neurons retrogradely labeled from the tooth pulp and facial skin, respectively, exhibited ASIC3-IR; 36% of CGRP-IR TG neurons which innervate these tissues co-expressed ASIC3-IR. Only 4% of ASIC3-IR cutaneous TG neurons showed PV-IR, while 25% of ASIC3-IR tooth pulp neurons were also immunoreactive for PV. The present study suggests that ASIC3-IR TG neurons supply the tooth pulp and facial skin with unmyelinated or finely myelinated axons. ASIC3-IR neurons which have large myelinated axons may be common in the tooth pulp but not the facial skin. The axonal morphology of ASIC3-IR TG neurons may depend on the variety of their receptive fields.
Ichikawa
Hiroichi
H
Department of Oral Function and Anatomy and Biodental Research Center, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8525, Japan. hiroichi@md.okayama-u.ac.jp
Sugimoto
T
T
D062565:Acid Sensing Ion Channels; D000138:Acidosis / Q000378:metabolism; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D003786:Dental Pulp Cavity / Q000294:innervation / Q000503:physiopathology; D005145:Face / Q000294:innervation / Q000502:physiology; D005157:Facial Pain / Q000378:metabolism* / Q000503:physiopathology; D005455:Fluorescent Antibody Technique; D008297:Male; D008565:Membrane Proteins; D009055:Mouth / Q000294:innervation / Q000502:physiology; D009412:Nerve Fibers / Q000378:metabolism / Q000648:ultrastructure; D009419:Nerve Tissue Proteins; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism*; D009619:Nociceptors / Q000166:cytology / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000294:innervation / Q000503:physiopathology; D015222:Sodium Channels / Q000378:metabolism*; D012668:Trigeminal Ganglion / Q000166:cytology / Q000378:metabolism*
C112231:ASIC3 protein, human; C568269:ASIC3 protein, rat; D062565:Acid Sensing Ion Channels; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D015222:Sodium Channels; D015740:Calcitonin Gene-Related Peptide