Detection of a promoter polymorphism in the gene of intestinal fatty acid binding protein (I-FABP).
Postprandial fat absorption is supposed to be a major factor in the development of the metabolic syndrome. In recent years, the assimilation of plasma triglycerides has been the focus of several groups, revealing a number of specific fat or fatty acid transporters. The intestinal fatty acid binding protein, I-FABP-2, participates in the absorption of nutritional fats. The influence of a coding polymorphism has been investigated intensively. However, it remains still unclear whether this polymorphism has a major impact on postprandial TG levels in humans. We found a polymorphism in the promoter of FABP-2, which might involve the retinoid receptor in the transcriptional activity. In functional analysis, we have been able to demonstrate that the various promoter alleles develop different activities in the human intestinal epithelial cells and that the postprandial appearance of plasma TGs in healthy subjects also depends on their genotype. Since the distribution of the identified promoter polymorphism does not differ in subjects suffering from type 2 diabetes, the overall influence on the development of the metabolic syndrome seems to be minor.
Geschonke
K
K
Institute of Physiology and Biochemistry of Nutrition, Federal Research Center, 24103 Kiel, Germany.
Klempt
M
M
Lynch
N
N
Schreiber
S
S
Fenselau
S
S
Schrezenmeir
J
J
D000328:Adult; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D016022:Case-Control Studies; D002460:Cell Line; D004247:DNA; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D014280:Triglycerides / Q000097:blood; D025521:Tumor Suppressor Proteins
D002352:Carrier Proteins; C493404:FABP2 protein, human; C107608:FABP7 protein, human; C493405:Fabp2 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D014280:Triglycerides; D025521:Tumor Suppressor Proteins; D004247:DNA