title: Complement-mediated neutralization of canine distemper virus in vitro: cross-reaction between vaccine Onderstepoort and field KDK-1 strains with different hemagglutinin gene characteristics.
abstract: The properties of neutralization of antigens of canine distemper virus Onderstepoort and a recent field isolate, KDK-1, were investigated with strain-specific dog sera. A conventional neutralization assay indicated antigenic dissimilarity between the strains; however, when guinea pig complement was included in the reaction mixture, the strains were neutralized with not only the homologous but also the heterologous antibodies.
authors.affiliation: Laboratory of Clinical Microbiology, Kyoritsu Seiyaku Corporation, 1-12-4 Kudankita, Chiyoda-ku, Tokyo 102-0073, Japan. msmmchzk@mb.infoweb.ne.jp
authors.forename: Masami
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Megumi
authors.initials: M
authors.lastname: Motoyoshi
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
authors.forename: Kazunari
authors.initials: K
authors.lastname: Kai
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000940:Antigenic Variation; D003165:Complement System Proteins / Q000276:immunology*; D003429:Cross Reactions; D004216:Distemper / Q000276:immunology / Q000821:virology; D004217:Distemper Virus, Canine / Q000145:classification / Q000235:genetics / Q000276:immunology*; D004285:Dogs; D006389:Hemagglutinins, Viral / Q000235:genetics*; D009500:Neutralization Tests; D014612:Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D014612:Vaccines; D003165:Complement System Proteins