title: Bacteriophage T4 development in Escherichia coli is growth rate dependent.
abstract: Three independent parameters (eclipse and latent periods, and rate of ripening during the rise period) are essential and sufficient to describe bacteriophage development in its bacterial host. A general model to describe the classical "one-step growth" experiment [Rabinovitch et al. (1999a) J. Bacteriol.181, 1687-1683] allowed their calculations from experimental results obtained with T4 in Escherichia coli B/r under different growth conditions [Hadas et al. (1997) Microbiology143, 179-185]. It is found that all three parameters could be described by their dependence solely on the culture doubling time tau before infection. Their functional dependence on tau, derived by a best-fit analysis, was used to calculate burst size values. The latter agree well with the experimental results. The dependence of the derived parameters on growth conditions can be used to predict phage development under other experimental manipulations.
authors.affiliation: Department of Physics, Ben-Gurion University of the Negev, Be'er-Sheva 84105, Israel.
authors.forename: Avinoam
authors.initials: A
authors.lastname: Rabinovitch
authors.forename: Itzhak
authors.initials: I
authors.lastname: Fishov
authors.forename: Hilla
authors.initials: H
authors.lastname: Hadas
authors.forename: Monica
authors.initials: M
authors.lastname: Einav
authors.forename: Arieh
authors.initials: A
authors.lastname: Zaritsky
mesh_terms: D017122:Bacteriophage T4 / Q000254:growth & development*; D004926:Escherichia coli / Q000821:virology*; D008954:Models, Biological; D014779:Virus Replication / Q000502:physiology*