Intracerebral hemorrhage upregulates Na(+)/myo-inositol cotransporter in the rat brain.
We examined the expression of Na(+)/myo-inositol cotransporter (SMIT) in the rat brain after intrastriatal hemorrhage. The expression of SMIT messenger RNA (mRNA) increased around hematoma 3 days after hemorrhage and it returned to control level as hematoma was absorbed. The expression of SMIT mRNA also increased at ipsilateral substantia nigra without blood-brain barrier disruption 7 days after hemorrhage and remained high until 42 days after hemorrhage. Immunohistochemistry revealed that macrophages around hematoma and microglias at ipsilateral substantia nigra were SMIT-positive. These results suggest that the expression of SMIT must be regulated not only by osmolality but also by unknown factors.
Aihara
Noritaka
N
Department of Neurosurgery, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8602, Japan. aihara@med.nagoya-cu.ac.jp
Imamura
Nobuki
N
Kimura
Tomohiro
T
Yamada
Kazuo
K
Hida
Hideki
H
Nishino
Hitoo
H
Ueda
Takashi
T
Shimada
Shoichi
S
D000818:Animals; D001812:Blood-Brain Barrier; D002543:Cerebral Hemorrhage / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000502:physiology; D006360:Heat-Shock Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D008264:Macrophages / Q000737:chemistry; D008297:Male; D008565:Membrane Proteins; D017628:Microglia / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000098:blood supply / Q000166:cytology / Q000502:physiology*; D027981:Symporters / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology
D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D027981:Symporters; C073884:SLC5A3 protein, human