[Antibody-based diagnostic assays with recombinant virus-like particles].
Virus-like particles were produced with 4 genogroup I Norwalk-like viruses(NLVs) and 7 genogroup II NLVs by a baculovirus expression system, and used to detect the antibody to NLVs by enzyme-linked immunosorbent assays(ELISA). Very little cross reactivity was observed between the genogroup I NLVs and the genogroup II NLVs when the antibody ELISA was done with hyperimmune sera. Infections by several genotypes of the NLVs were thought to occur in oyster-associated acute gastroenteritis, while an infection by a single genotype of NLVs was found in the illness occurred in a hospital. A high prevalence of antibody to 11 NLVs was observed in samples collected from healthy adults in Japan.
Natori
Katsuro
K
Department of Virology II, National Institute of Infectious Diseases.
D000914:Antibodies, Viral / Q000032:analysis*; D017250:Caliciviridae Infections / Q000175:diagnosis* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D029322:Norovirus / Q000276:immunology; D011995:Recombination, Genetic; D012698:Serologic Tests / Q000379:methods; D014771:Virion / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral