Optimisation of the degenerate oligonucleotide primed PCR (DOP-PCR) for capillary thermocycler.
DOP-PCR was optimised for capillary thermocycler. Five primers with five or six nucleotide anchor sequences and different restriction enzyme recognition sites were tested. The efficiency of the amplification was confirmed by gel electrophoresis and fluorescence in situ hybridisation on metaphase chromosomes.
Kiss
Csaba
C
Microbiology and Tumor Biology Center (MTC), Karolinska Institute, Nobels vag 16, S-17177 Stockholm, Sweden. csaba.kiss@mtc.ki.se
Kost-Alimova
Maria
M
Klein
George
G
Szekely
Laszlo
L
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D011786:Quality Control; D017384:Sequence Deletion; D019914:Taq Polymerase / Q000235:genetics*; D013696:Temperature
D017931:DNA Primers; D019914:Taq Polymerase