Expansion of CD5 - B cells in multiple sclerosis correlates with CD80 (B7-1) expression.
The pathogenetic role of autoantibodies in multiple sclerosis (MS) is uncertain. CD5+ B cells commonly produce autoantibodies, but CD5 expression has also been implicated in B-cell tolerance. We studied B-cell subsets, anti-myelin protein antibody-secreting cells in cerebrospinal fluid (CSF) and a panel of serum autoantibodies in patients with clinically isolated syndromes (CIS), suggestive of MS and patients with clinically definite MS (CDMS). Patients with CDMS had a higher percentage of CD5- B cells in CSF than did control subjects (P = 0.02). CIS patients with immunoglobulin G (IgG) oligoclonal bands in CSF or multiple lesions on magnetic resonance imaging (MRI) had a higher percentage of CD5- B cells in CSF than did the remaining CIS patients (P = 0.03). The percentage of CD5- and CD80+ B cells correlated positively and the percentage of CD5+ B cells correlated negatively with the number of CSF cells secreting anti-myelin basic protein (anti-MBP) antibodies. The prevalence of serum autoantibodies was comparable in the three patient groups. We conclude that intrathecal expansion of CD5- B cells appears to be more characteristic in MS patients, and CD5+ B cells may be associated with a lower prevalence of anti-myelin antibody production.
Sellebjerg
F
F
Department of Neurology, University of Copenhagen, Glostrup Hospital, Denmark. sellebjerg@dadlnet.dk
Jensen
J
J
Jensen
C V
CV
Wiik
A
A
D000328:Adult; D001323:Autoantibodies / Q000097:blood; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D018122:B7-1 Antigen / Q000096:biosynthesis*; D018956:CD5 Antigens / Q000096:biosynthesis / Q000276:immunology*; D005260:Female; D006801:Humans; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology*; D004676:Myelin Basic Protein / Q000276:immunology; D018991:Myelin Proteolipid Protein / Q000276:immunology
D001323:Autoantibodies; D018122:B7-1 Antigen; D018956:CD5 Antigens; D004676:Myelin Basic Protein; D018991:Myelin Proteolipid Protein