title: Sequence-specific molecular lithography on single DNA molecules.
abstract: Recent advances in the realization of individual molecular-scale electronic devices emphasize the need for novel tools and concepts capable of assembling such devices into large-scale functional circuits. We demonstrated sequence-specific molecular lithography on substrate DNA molecules by harnessing homologous recombination by RecA protein. In a sequence-specific manner, we patterned the coating of DNA with metal, localized labeled molecular objects and grew metal islands on specific sites along the DNA substrate, and generated molecularly accurate stable DNA junctions for patterning the DNA substrate connectivity. In our molecular lithography, the information encoded in the DNA molecules replaces the masks used in conventional microelectronics, and the RecA protein serves as the resist. The molecular lithography works with high resolution over a broad range of length scales from nanometers to many micrometers.
authors.affiliation: Department of Physics, Solid State Institute, Technion-Israel Institute of Technology, Haifa 32000, Israel.
authors.forename: Kinneret
authors.initials: K
authors.lastname: Keren
authors.forename: Michael
authors.initials: M
authors.lastname: Krueger
authors.forename: Rachel
authors.initials: R
authors.lastname: Gilad
authors.forename: Gdalyahu
authors.initials: G
authors.lastname: Ben-Yoseph
authors.forename: Uri
authors.initials: U
authors.lastname: Sivan
authors.forename: Erez
authors.initials: E
authors.lastname: Braun
mesh_terms: D000906:Antibodies; D001704:Biopolymers; D001710:Biotin; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004553:Electric Conductivity; D004581:Electronics; D005976:Glutaral; D006046:Gold; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization; D011938:Rec A Recombinases / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D011995:Recombination, Genetic; D012834:Silver; D019809:Streptavidin; D013698:Templates, Genetic
chemical_list: D000906:Antibodies; D001704:Biopolymers; D004277:DNA, Single-Stranded; D012834:Silver; D001710:Biotin; D006046:Gold; D004247:DNA; D019809:Streptavidin; D011938:Rec A Recombinases; D005976:Glutaral