title: Involvement of Candida albicans NADH dehydrogenase complex I in filamentation.
abstract: The gene encoding the 51-kDa subunit of nicotinamide adenine dinucleotide (NADH) dehydrogenase complex I, a principal component of the mitochondrial electron transport chain, was cloned in Candida tropicalis. The homolog in C. albicans, CaNDH51, was identified, and each allele was successively disrupted by PCR-mediated gene disruption. Wild type, heterozygote, reintegrant, and homozygous null mutants grew as blastoconidia in rich medium containing 3% glucose, but the homozygous null mutant failed to grow in ethanol or acetate. When glucose concentration was varied from 1 mM (0.018%) to 200 mM (3.6%) in a basal salts medium, all strains grew equally well at all glucose concentrations; the wild-type strain, the heterozygote, and the reintegrant exhibited abundant germ tubes, pseudohyphae, and hyphae. In contrast, the ndh51/ndh51 strain failed to display any type of filamentous growth, even in glucose concentrations as low as 1 mM. These results suggest a previously unexplored relationship between mitochondrial electron transport and morphogenesis.
authors.affiliation: Department of Pediatrics and Center for Microbial Pathogenesis, Yale University School of Medicine, 464 Congress Avenue, New Haven, CT 06519, USA.
authors.forename: Justin A
authors.initials: JA
authors.lastname: McDonough
authors.forename: Vasker
authors.initials: V
authors.lastname: Bhattacherjee
authors.forename: Tania
authors.initials: T
authors.lastname: Sadlon
authors.forename: Margaret K
authors.initials: MK
authors.lastname: Hostetter
mesh_terms: D000595:Amino Acid Sequence; D002176:Candida albicans / Q000201:enzymology* / Q000254:growth & development; D017931:DNA Primers; D008928:Mitochondria / Q000502:physiology; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000502:physiology*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D017931:DNA Primers; D009245:NADH Dehydrogenase