title: [Molecular screening of MC4R gene and association with fat traits in pig resource family].
abstract: Melanocortin-4 Receptor (MC4R) plays an important role in the regulation of human obesity. It can cooperate with leptin, neuropeptide Y(NPY) and melanocyte-stimulating hormone (MSH) to regulate body weight and feeding. Inactivation of this receptor by gene targeting in mice results in a maturity onset obesity syndrome associated with hyperphagic, hyperinsulinemia, hyperglycemia, as well as decreased linear growth and adult obesity. Multiple alignments of the sequences from individuals of several pig lines identified a single nucleotide substitution(G-->A) at position 298 of the seventh transmembrane domain. In present study, polymorphism distribution of MC4R gene fragment in resource population was studied using PCR-RFLP method based on the enzyme Taq I. The genotype was analyzed with the phenotype of the slaughtered individuals. The results showed that the frequencies of MC4R genotype varied in different breeds. The correlation analysis demonstrated the genotype of MC4R was in significant relation with back-fat thickness on thorax-waist, buttock and the average back-fat thickness, as well as with the width and area of longissmus dorsi (LD), and the percentage of skin. MC4R gene plays a role mainly in the pattern of dominant effect, and all the additive effects were not significant.
authors.affiliation: Key Laboratory of Pig Genetics and Breeding of the Agricultural Ministry, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Gui-Lan
authors.initials: GL
authors.lastname: Liu
authors.forename: Si-Wen
authors.initials: SW
authors.lastname: Jiang
authors.forename: Yuan-Zhu
authors.initials: YZ
authors.lastname: Xiong
authors.forename: Rong
authors.initials: R
authors.lastname: Zheng
authors.forename: Yan-Chun
authors.initials: YC
authors.lastname: Qu
mesh_terms: D000818:Animals; D005223:Fats / Q000378:metabolism*; D005787:Gene Frequency; D005820:Genetic Testing; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D044105:Receptor, Melanocortin, Type 4; D018000:Receptors, Peptide / Q000235:genetics*; D013552:Swine / Q000145:classification / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
chemical_list: D005223:Fats; D044105:Receptor, Melanocortin, Type 4; D018000:Receptors, Peptide