The Sequence Analysis and in Vitro Cleavage by Ribozymes of a Putative Early and Late Transcription Gene of the Prawn Baculovirus.
Since 1993 there has been outbreak of an acute lethal disease of the cultivated fleshy prawns (Penaeus chinesis, Osbeck) in China. After a short period of intensive studies we reported firstly the presence of a non-occluded baculovirus in the tissue of diseased prawns. The virus was also named as white spot syndrome baculovirus by some authors. Here, it is reported that, based on sequence analysis, an early and late transcription gene(1 197bp gene)of baculovirus was identified. Two hammerhead ribozymes RZ1 and RZ2 targeting the 54-56 bp and 314-316 bp of the 1 197 bp gene were designed, and in vitro cleavage experiments showed that under proper conditions the target gene could be completely cleaved by those ribozymes together or separately.
Zhou
Guo-Ying
GY
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. gongzx@sunm.shcnc.ac.cn
Wu
Jian-Hua
JH
Shen
Xue-Ren
XR
Gong
Zu-Xun
ZX