KIAA0202, a human septin family member, interacting with hPFTAIRE1.
hPFTAIRE1 is a member of Cdc2-related kinase family localized in cytoplasm. To search its substrates and regulatory proteins, the hPFTAIRE1 was fused to LexA and used as bait to screen a human brain LexA two-hybrid library. In this screening, 7 hPFTAIRE1 interacting proteins, including KIAA0202, were obtained. The interaction between the KIAA0202 and the hPFTAIRE1 was confirmed by using immunoprecipitation. The KIAA0202 is a 508 aa protein and is a member of septin family. The central portion of the KIAA0202 is a GTP-CDC domain, which is conserved among almost all septins. Its C-terminus is a coiled-coil structure and its N-terminus is a variable region. Our results suggest that the KIAA0202 may play important roles in functional regulation of hPFTAIRE1.
Yang
Tao
T
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China.
Gao
Yan-Kun
YK
Chen
Jiang-Ye
JY
D000595:Amino Acid Sequence; D001921:Brain / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D058112:Septins; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques
D008565:Membrane Proteins; D011494:Protein Kinases; C112141:Pftk1 protein, mouse; D020558:GTP Phosphohydrolases; C464445:SEPTIN8 protein, human; D058112:Septins; C407343:septin 3