Culturing precision-cut human prostate slices as an in vitro model of prostate pathobiology.
Due to the complex morphology of the prostate, it was hypothesized that precision-cut tissue slices from human prostate would provide a unique in vitro model. Precision-cut slices were generated from zones of human prostate and their viability was assessed under conditions of different media for up to 120 h. Slices were also exposed to several concentrations of CdCI2, which was used as a model toxicant. Maintenance of both stromal and epithelial cells was noted; however, there was a gradual loss of luminal epithelial cells when the medium was not supplemented with dihydrotestosterone (DHT). Minimal leakage of lactate dehydrogenase occurred throughout the incubation. Prostate-specific antigen (PSA) was detected in the medium at all time points, although the rates of secretion fell over time. There was a loss of PSA-positive cells when the medium was not supplemented with DHT, consistent with a loss of luminal cells, whereas PSA-positive cells were maintained in the DHT-supplemented media. A proliferation of basal cells was observed in the presence of media containing 10% fetal bovine serum. Exposure of slices to CdCl2 demonstrated a dose-response effect ranging from proliferation to complete cellular necrosis. Given the retention of stromal-epithelial interactions and the use of acquired human tissue, prostate slices represent a unique in vitro model for investigating human prostate pathobiology.
Parrish
A R
AR
Department of Medical Pharmacology, Texas A&M University, College Station, USA.
Sallam
K
K
Nyman
D W
DW
Orozco
J
J
Cress
A E
AE
Dalkin
B L
BL
Nagle
R B
RB
Gandolfi
A J
AJ
D019256:Cadmium Chloride / Q000633:toxicity; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D046508:Culture Techniques; D006801:Humans; D008297:Male; D008954:Models, Biological; D011467:Prostate / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D017430:Prostate-Specific Antigen / Q000378:metabolism
D017430:Prostate-Specific Antigen; D019256:Cadmium Chloride