title: [Genotyping of Plasmodium falciparum isolates by amplification of glutamate-rich protein gene using polymerase chain reaction].
abstract: OBJECTIVE
  To develop a genotyping method based on amplifying glutamate-rich protein (GLURP) gene for the diagnosis and identification of Plasmodium falciparum.
  
  
  METHODS
  Two pairs of primers specific for GLURP gene of P. falciparum were designed and synthesized. R2 polymorphic domain of GLURP gene was amplified by nested PCR, which was applied to genotyping of P. falciparum isolates obtained from patients attending the malaria clinic at the village of Borai, Thailand.
  
  
  RESULTS
  Conspicuous polymorphism of GLURP alleles in natural populations of P. falciparum was found. 290 GLURP alleles were detected in 154 P. falciparum infections. Among the above-mentioned alleles, 12 different GLURP genotypes were distinguished according to different DNA sizes. Of them, the most frequently found allele was a variant of 770 bp, the least allele was that of 1,100 bp. More than 43% of the patients were found to be infected with mixed alleles. No apparent change for frequencies of the 12 different alleles was found in the 9-month longitudinal study.
  
  
  CONCLUSIONS
  A genotyping method is developed for the research of strain taxonomy and pathogenesis of malaria parasites.
authors.affiliation: Capital University of Medical Sciences, Beijing 100054.
authors.forename: X
authors.initials: X
authors.lastname: Zhu
authors.forename: G
authors.initials: G
authors.lastname: Snounou
authors.forename: W
authors.initials: W
authors.lastname: Jarra
authors.forename: S
authors.initials: S
authors.lastname: Thaithong
authors.forename: K N
authors.initials: KN
authors.lastname: Brown
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics*; D017125:Genes, Protozoan; D005838:Genotype; D006801:Humans; D008137:Longitudinal Studies; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; C072492:glutamate-rich protein, Plasmodium