[Influence of modulatory activity of wound fluid on the characteristics of fibroblasts from irradiation and the action of phenytoin sodium].
OBJECTIVE
Influence of irradiation and phenytoin sodium on modulatory activities of wound fluid on proliferation of fibroblasts and collagen synthesis was studied.


METHODS
The male Wistar rats were used in this study. The rats were divided into irradiated and non-irradiated groups, and in each of them it was subdivided into phenytoin group and control. A 7 cm long incisional wound was made on the back of each rat, in which a polyvinyl alcohol sponge (PVAS) with a size of 1.0 cm x 0.4 cm was implanted into the wound and the wound was sutured up. The PVAS was prepared by rinsing in running water over night and then was boiled for 30 minutes. Before implantation, the sponge was immersed in phenytoin sodium solution (10 mg/l ml) or normal saline (as control). From each wound the wound fluid and fibroblasts were collected. The methods of incorporation of 3H were adopted to assess the proliferation of fibroblasts and synthesis of collagen.


RESULTS
It was shown that proliferation of fibroblasts and collagen synthesis were stimulated by wound fluid remarkably on 5 to 8 days after wounding, and that 6 Gy to total-body irradiation wound decrease this effect. It was also noted that topical phenytoin sodium increased the modulatory activity of wound fluid irrespective of being irradiated or not.


CONCLUSIONS
It could be drawn that, after total-body irradiation, stimulation of hyperplasia of fibroblasts and collagen synthesis by wound fluid was markedly lowered indicating the total-body irradiation resulted in changes of local conditions of the wound which was unbenefitted to repair of tissue cells, while phenytoin sodium could enhance the stimulating action of wound fluid on proliferation of fibroblasts and synthesis of collagen which was beneficial to wound healing.
Song
S
S
Department of Clinical Pharmacology, General Hospital of Guangzhou Military Area, Guangzhou, Guangdong, P. R. China 510010.
Cheng
T
T
Lin
Y
Y
D000818:Animals; D002455:Cell Division; D003037:Cobalt Radioisotopes; D003094:Collagen / Q000096:biosynthesis; D005122:Exudates and Transudates / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D008297:Male; D010672:Phenytoin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D014916:Whole-Body Irradiation; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology; D014947:Wounds and Injuries / Q000473:pathology
D003037:Cobalt Radioisotopes; D010672:Phenytoin; D003094:Collagen; C043114:fosphenytoin