[Sensitivity and specificity of nested polymerase chain reaction in detecting Plasmodium vivax in infected mosquitoes].
OBJECTIVE
To study the sensitivity and specificity of nested polymerase chain reaction for detection of Plasmodium vivax in infected mosquitoes.


METHODS
Two pairs of primers specific to small subunit ribosomal DNA of P. vivax were used to amplify the specific SSUrDNA 121 bp fragment of P. vivax for detecting P. vivax-infected mosquitoes with nested PCR.


RESULTS
Nested PCR could detect as few as 3 sporozoites in one mosquito or 1 infected mosquito mixed with a group of 99 normal ones. In contrast, no such specific 121 bp DNA band was detected in P. falciparum, P. cynomolgi, P. yoelii yoelii infected samples, nor in normal mosquito.


CONCLUSIONS
The nested PCR technique we established showed high sensitivity and specificity.
Li
F
F
Department of Parasitology, Capital University of Medical Sciences, Beijing 100054.
Niu
C
C
Ye
B
B
Zhu
X
X
Chen
P
P
D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D016054:DNA, Protozoan / Q000032:analysis*; D006801:Humans; D010966:Plasmodium vivax / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D016054:DNA, Protozoan