Embryonic development in the eggs of the silkworm, Bombyx mori, exposed to the space environment.
To investigate the effects of cosmic radiation and microgravity on embryogenesis and organogenesis in Bombyx eggs, two different stages of eggs, the early stage after oviposition and the diapause-terminated eggs, were loaded on the US Space Shuttle/Atlantis (STS-84) for a 9 day flight. More than 85% of the early stage eggs hatched in the flight sample and the ground control. In the diapause-terminated eggs, the percentage of unhatched eggs were 43% in the ground control and 56% in the flight sample. In these eggs, uncompleted embryonic reversal was observed two-fold higher percentage in the flight sample than in the ground control. The incidence of abnormality such as the larvae with segmental fusion and the appearance of abnormal crescent marking in the flight sample was significantly higher than that in the ground control. This was also observed in the 1st and 2nd filial generation of the flight sample. From these results, unsuccessful blastokinesis and the abnormal appearance was discussed in relation to cosmic radiation and microgravity.
Furusawa
T
T
Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan.
Kotani
E
E
Ichida
M
M
Sugimura
Y
Y
Yamanaka
H
H
Takahashi
S
S
Fukui
M
M
Kogure
K
K
Sakaguchi
B
B
Fujii
H
H
Ikenaga
M
M
Watanabe
T
T
D000818:Animals; D012831:Bombyx / Q000196:embryology* / Q000254:growth & development / Q000528:radiation effects; D003359:Cosmic Radiation; D004307:Dose-Response Relationship, Radiation; D004625:Embryo, Nonmammalian; D005260:Female; D007814:Larva / Q000254:growth & development / Q000528:radiation effects; D008297:Male; D008675:Metamorphosis, Biological / Q000502:physiology / Q000528:radiation effects*; D011829:Radiation Dosage; D013026:Space Flight; D014893:Weightlessness; D015053:Zygote / Q000254:growth & development* / Q000528:radiation effects