Induction of an outer membrane protein of 78 kDa in Vibrio vulnificus cultured in the presence of desferrioxamine B under iron-limiting conditions.
Although Vibrio vulnificus is known to be able to utilize ferrioxamine B as an iron source, its outer membrane receptor remains to be determined. In this study, we found that V. vulnificus expressed a new outer membrane protein of 78 kDa when grown in the presence of desferrioxamine B under iron-limiting conditions. The desferrioxamine B-dependent iron uptake was only observed in bacterial cells expressing this protein. Furthermore, non-denaturing polyacrylamide gel electrophoresis followed by autoradiography of the outer membrane preparation containing the 78-kDa protein preincubated with [(55)Fe]ferrioxamine B provided a single radioactive band in which the 78-kDa outer membrane protein was present as the major component. These lines of evidence suggest that the inducible 78-kDa protein may serve as the cell-surface receptor for ferrioxamine B.
Aso
Hiroaki
H
Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-8530, Japan.
Miyoshi
Shin-ichi
S
Nakao
Hiroshi
H
Okamoto
Keinosuke
K
Yamamoto
Shigeo
S
D000595:Amino Acid Sequence; D001345:Autoradiography; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D003470:Culture Media; D003676:Deferoxamine / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007501:Iron / Q000378:metabolism*; D014733:Vibrio / Q000254:growth & development* / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D007501:Iron; D003676:Deferoxamine