Synthesis and applications of end-labeled neoglycopolymers.
[reaction: see text] Neoglycopolymers that vary in length and contain a single fluorescent reporter group were synthesized using ring-opening metathesis polymerization (ROMP). The utility of these materials is demonstrated by the development of a cellular binding assay for L-selectin, a cell surface protein that plays a role in inflammation. The data reveal that these multivalent ligands interact with multiple copies of L-selectin.
Owen
Robert M
RM
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Gestwicki
Jason E
JE
Young
Travis
T
Kiessling
Laura L
LL
D001665:Binding Sites; D003517:Cyclopentanes / Q000138:chemical synthesis; D019040:E-Selectin / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005690:Galactose / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D006801:Humans; D007202:Indicators and Reagents; D019169:Jurkat Cells; D007962:Leukocytes / Q000737:chemistry; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008562:Membrane Glycoproteins / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis*
D003517:Cyclopentanes; D019040:E-Selectin; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008024:Ligands; D008562:Membrane Glycoproteins; D011108:Polymers; D005690:Galactose