title: An enzymatic fluorometric assay for pyridoxal with high specificity and sensitivity.
abstract: An enzymatic fluorometric assay for pyridoxal with pyridoxal dehydrogenase was developed. The detection limit was about 10 pmol: the calibration curve of pyridoxal showed high linearity (r=0.993). The values obtained by this method correlated well with those by the HPLC method. The enzyme had a high specificity for pyridoxal, and thus animal samples could be directly analyzed without separation of pyridoxal 5'-phosphate by column chromatography.
authors.affiliation: Department of Bioresources Science, Faculty of Agriculture, Kochi University, Japan.
authors.forename: Yanee
authors.initials: Y
authors.lastname: Trongpanich
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Mito
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Yagi
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D011730:Pyridoxal / Q000032:analysis*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D011730:Pyridoxal; D000429:Alcohol Oxidoreductases; C042642:pyridoxal dehydrogenase