Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo.
In order to track hematopoetic cells of all lineages unambiguously at all stages of development, we have developed C57BL/6 mice that express a transgene coding for green fluorescent protein (GFP) under control of the human ubiquitin C promoter. These mice, called UBI-GFP/BL6, express GFP in all tissues examined, with high levels of GFP expression observed in hematopoetic cells. UBI-GFP/BL6 mice are unique in that B cells, T cells, and dendritic cells have distinct levels of GFP fluorescence. In cell transfer experiments, leukocytes from UBI-GFP/BL6 mice are readily identified by FACS or fluorescence microscopy. We demonstrate that transplanted UBI-GFP/BL6 dendritic cells are easily identified in secondary lymphoid tissues. Direct interactions between individual dendritic cells and multiple naïve CD8+ T cells are observed in lymph nodes within 12 h of cell transfer and require loading of the dendritic cells with the appropriate peptide antigen. Dendritic cells undergo specific morphologic changes following interactions with antigen-specific T cells.
Schaefer
B C
BC
National Jewish Medical and Research Center, 1400 Jackson Street, K512, Denver, Colorado 80206, USA. schaeferb@njc.org
Schaefer
M L
ML
Kappler
J W
JW
Marrack
P
P
Kedl
R M
RM
D019264:Adoptive Transfer; D000818:Animals; D017951:Antigen Presentation; D000941:Antigens / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002450:Cell Communication; D019070:Cell Lineage; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D003713:Dendritic Cells / Q000276:immunology*; D005456:Fluorescent Dyes / Q000032:analysis*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000032:analysis*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D011828:Radiation Chimera; D011993:Recombinant Fusion Proteins / Q000032:analysis; D013154:Spleen / Q000166:cytology / Q000276:immunology; D025822:Ubiquitin C / Q000235:genetics
D000941:Antigens; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D025822:Ubiquitin C; D049452:Green Fluorescent Proteins