[Novel renal function marker, ATP--establishing the normal range, cases of anti-tumor drags administration for urinary-track tumor, diabetic diseases and a newborn baby].
Urinary free ATP assay by the firefly luciferin-luciferase method is a rapid and simple method for determining renal function, especially uriniferous tubule function. Normal range of urinary free ATP concentration, daily ATP excretion in urine, urinary ATP/creatinine value and ATP decomposition activity in urine is 1.1 x 10(-9)-3.4 x 10(-8) M, 4.0 x 10(-9)-4.1 x 10(-8) mole, 5.0 x 10(-13)-5.9 x 10(-11) mol/mgCr and 100-77% express for the remaining rate of additional ATP, respectively. A significant correlation was found between free ATP concentration and daily ATP excretion in urine with a correlation coefficient of 0.84. In cases of anti-tumor drug(cisplatin = cis-diamminedichloroplatinum II) administration for urinary-track tumor, abnormal urinary free ATP concentration and ATP decomposition activity in urine were clearly demonstrated after a few days of cisplatin administration. The appearance of a tendency toward abnormal relative ATP values were similar to changes in beta 2-MG and NAG values. Diabetic patients often demonstrate unusually high values of urinary free ATP concentration. In asphyxia of the newborn, urinary ATP/creatinine value were significantly higher than those in healthy newborn, but urinary NAG values did not differ.
Nakajima
Moto-o
MO
Research & Development Division, Kikkoman Co., Noda 278-0037.
Etoh
Yuki
Y
Yanai
Mitsuru
M
Kawamura
Risako
R
Kaneko
Kazunari
K
Aoyagi
Kazumasa
K
Koiso
Kenkiti
K
D000255:Adenosine Triphosphate / Q000652:urine*; D000328:Adult; D000970:Antineoplastic Agents / Q000008:administration & dosage; D001238:Asphyxia Neonatorum / Q000652:urine; D015415:Biomarkers / Q000652:urine; D002945:Cisplatin / Q000008:administration & dosage; D003920:Diabetes Mellitus / Q000652:urine*; D005260:Female; D006801:Humans; D007231:Infant, Newborn / Q000652:urine*; D007668:Kidney / Q000503:physiopathology*; D008297:Male; D012016:Reference Values; D014571:Urologic Neoplasms / Q000188:drug therapy / Q000652:urine*
D000970:Antineoplastic Agents; D015415:Biomarkers; D000255:Adenosine Triphosphate; D002945:Cisplatin