title: Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes.
abstract: We used microarrays and real-time reverse transcription-PCR to analyze the global transcriptional response of Mycobacterium tuberculosis to low pH in vitro, which may mimic an environmental signal encountered by phagocytosed mycobacteria. Eighty-one genes were differentially expressed >1.5-fold, including many involved in fatty acid metabolism. The most highly induced genes showed homology with nonribosomal peptide synthetases/polyketide synthases.
authors.affiliation: Program in Microbiology and Molecular Genetics, Emory University, Atlanta, Georgia 30322, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Fisher
authors.forename: Bonnie B
authors.initials: BB
authors.lastname: Plikaytis
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Shinnick
mesh_terms: D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism; D009097:Multienzyme Complexes / Q000096:biosynthesis; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010453:Peptide Synthases / Q000096:biosynthesis; D010588:Phagosomes / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D009097:Multienzyme Complexes; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase