title: Microdeletions in the Y chromosome of patients with idiopathic azoospermia.
abstract: OBJECTIVE
  To evaluate the occurrence and prevalence of microdeletions in the gamma chromosome of patients with azoospermia.
  
  
  METHODS
  DNA from 29 men with idiopathic azoospermia was screened by polymerase chain reaction (PCR) analysis with a set of gamma chromosome specific sequence-tagged sites (STSs) to determine microdeletions in the gamma chromosome.
  
  
  RESULTS
  Deletions in the DAZ (deleted in azoospermia) loci sgamma254 and sgamma255 were found in three patients with idiopathic azoospermia, resulting in an estimated frequency of deletions of 10.7% in idiopathic azoospermia men.
  
  
  CONCLUSIONS
  We conclude that PCR analysis is useful for the diagnosis of microdeletions in the Y chromosome, which is important when deciding the suitability of a patient for assisted reproductive technology such as testicular sperm extracion-intracytoplasmic sperm injection (TESE-ICSI).
authors.affiliation: Department of Urology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Akiyuki
authors.initials: A
authors.lastname: Shimizu
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Ichikawa
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Suzuki
authors.forename: Takako
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Imamoto
authors.forename: Satoko
authors.initials: S
authors.lastname: Kojima
authors.forename: Yukio
authors.initials: Y
authors.lastname: Naya
authors.forename: Akira
authors.initials: A
authors.lastname: Komiya
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Koichi
authors.initials: K
authors.lastname: Nagao
authors.forename: Kazukiyo
authors.initials: K
authors.lastname: Miura
authors.forename: Haruo
authors.initials: H
authors.lastname: Ito
mesh_terms: D000328:Adult; D001483:Base Sequence; D041322:Chromosomes, Human, Y; D017931:DNA Primers; D022041:Euchromatin / Q000235:genetics; D005640:Follicle Stimulating Hormone / Q000097:blood; D006570:Heterochromatin / Q000235:genetics; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009845:Oligospermia / Q000097:blood / Q000209:etiology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011388:Prolactin / Q000097:blood; D017384:Sequence Deletion / Q000235:genetics*; D016324:Sequence Tagged Sites; D013739:Testosterone / Q000097:blood
chemical_list: D017931:DNA Primers; D022041:Euchromatin; D006570:Heterochromatin; D013739:Testosterone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone