[Effects of Ganoderma lucidum (Leyss ex Fr) Karst compound on the proliferation and differentiation of K562 leukemic cells].
A series of experiments including cell culture and benzidine staining test were undertaken to investigate the effects of Ganoderma lucidum(Leyss ex Fr) Karst Compound(GLC) on the proliferation and differentiation of K562 leukemic cells. The results showed that different concentrations of GLC(from 4 mg.ml-1 to 12 mg.ml-1) could promote human bone marrow granulocyte-macrophage colony forming unit (CFU-GM) proliferation, but suppressed the growth of K562 leukemic cell colonies, and IC50 was 9.2 mg.ml-1. The data from liquid culture demonstrated that GLC could suppress K562 cells proliferation in a dose-dependent(from 4 mg.ml-1 to 20 mg.ml-1) and time-dependent(from 1-5 days) manner. K562 cells could be induced to differentiate into more mature erythrocytic cells by 4 mg.ml-1 and 8 mg.ml-1 GLC. It is concluded that GLC may be a good medicine for leukemia therapy.
Zhong
L
L
Research Laboratory of Blood Physiology, Hunan Medical University, Changsha 410078.
Jiang
D
D
Wang
Q
Q
D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division; D003114:Colony-Forming Units Assay; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells / Q000473:pathology; D020940:Reishi
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal