Determination of nicotinamide and 4-aminobenzoic acid in pharmaceutical preparation by LC.
A rapid, precise and time saving high performance liquid chromatographic method has been developed and validated for the simultaneous determination of nicotinamide and 4-aminobenzoic acid in pharmaceutical preparation. The method involves isocratic elution of mobile phase through column in a reverse phase chromatography with UV detection at 254 nm. The ranges of quantification for nicotinamide and 4-aminobenzoic acid were 11-34 microg ml(-1) and 37-113 microg ml(-1), respectively. Investigating specificity, linearity, precision, accuracy, robustness and ruggedness performed the validation of the method.
Khan
Abdul Rafiq
AR
HEJ Research Institute of Chemistry, International Center for Chemical Sciences, University of Karachi, 75270, Karachi, Pakistan.
Khan
Khalid Mohammad
KM
Perveen
Shahnaz
S
Butt
Naeem
N
D010129:4-Aminobenzoic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D009536:Niacinamide / Q000032:analysis*; D015203:Reproducibility of Results
D004338:Drug Combinations; D009536:Niacinamide; D010129:4-Aminobenzoic Acid