The C-terminus of myogenin, but not MyoD, targets upregulation of MEF2C expression.
The myogenic regulatory family of basic helix-loop-helix transcription factors, including MyoD and myogenin, functions cooperatively with the myocyte-specific enhancer binding factor 2 (MEF2) family during skeletal myogenesis. Previously, using aggregated P19 cells, we have shown that myogenin upregulates MEF2C expression while MyoD does not [Ridgeway et al., J. Biol. Chem. 275 (2000) 41-46]. In order to identify the domain of myogenin responsible for activating MEF2C expression, a series of chimeras of MyoD and myogenin were generated. Only chimeras containing the C-terminal region of myogenin were able to activate MEF2C in aggregated P19 cells, suggesting that the C-terminus of myogenin is responsible for the regulation of specific target genes.
Department of Biochemistry, Medical Sciences Building, The University of Western Ontario, London, ON, Canada.
Parker J
PJ
Rogerson
Mina
M
Jamali
Ilona S
IS
Skerjanc
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D005455:Fluorescent Antibody Technique; D064326:MEF2 Transcription Factors; D051379:Mice; D017570:MyoD Protein / Q000737:chemistry / Q000378:metabolism*; D018006:Myogenic Regulatory Factors / Q000378:metabolism*; D018008:Myogenin / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D064326:MEF2 Transcription Factors; C578041:Mef2c protein, mouse; D017570:MyoD Protein; C503801:Myog protein, mouse; D018006:Myogenic Regulatory Factors; D018008:Myogenin; D004247:DNA