[Adenoviral transduction of endostatin gene prevents the liver metastasis of colorectal carcinoma in postoperation].
OBJECTIVE
To study the prevention of colorectal cancer liver metastasis by adenoviral transduction of the endostatin gene.


METHODS
The recombinant adenovirus expressing endostatin was constructed. Its biological activities were surveyed in vitro, as determined in human umbilicus vein endothelium cell (HUVEC) proliferation inhibition, and in vivo, by reduction of liver metastasis.


RESULTS
HUVEC proliferation was obviously inhibited by the infecting supernatant of recombinant adenovirus. Persistent high serum levels of endostatin in peripheral blood, especially in the liver vein were observed. The production of liver metastasis was intervened.


CONCLUSIONS
The single injection in the vein of the recombinant adenovirus realizes the high effective and stable expression of endostatin in general body and liver, which brings about the ideal prevention of liver metastasis.
Luo
Chengyu
C
Department of General Surgery, Beijing Army General Hospital, Beijing 100700, China.
Zhao
Danning
D
Zeng
Sheng
S
Yang
Xiaoming
X
Liu
Yuanlin
Y
Liang
Long
L
Zhu
Xueguang
X
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000235:genetics / Q000627:therapeutic use*; D015179:Colorectal Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D043169:Endostatins; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology; D015316:Genetic Therapy; D005822:Genetic Vectors; D008113:Liver Neoplasms / Q000517:prevention & control* / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis / Q000517:prevention & control; D009368:Neoplasm Transplantation; D010446:Peptide Fragments / Q000235:genetics / Q000627:therapeutic use*; D014161:Transduction, Genetic
D043169:Endostatins; D010446:Peptide Fragments; D003094:Collagen