Temporal and spatial expression of two isoforms of the Dutt1/Robo1 gene in mouse development.
The mammalian homologue of the Drosophila axonal guidance receptor roundabout is expressed in a wide range of tissues. Here we show that alternative splicing of the Dutt1/Robo1 gene results in two mRNA transcripts with different signal peptides, which are differentially expressed throughout mouse embryogenesis. Since mice with a targeted deletion in the Dutt1/Robo1 gene have abnormal lung pathology, immunohistochemistry was used to identify the cellular expression pattern of Dutt1/Robo1 during lung development. Dutt1/Robo1 expression was widespread and diffuse in the lung at embryonic day 17.5 but became increasingly localised to the bronchial epithelium in newborn and adult mice.
Clark
Katherine
K
Department of Oncology, University of Cambridge, MRC Centre, Hills Road, CB2 2QH, Cambridge, UK. kjclark@mrc-lmb.ac.uk
Hammond
Ester
E
Rabbitts
Pamela
P
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001980:Bronchi / Q000254:growth & development / Q000378:metabolism*; D004848:Epithelium / Q000254:growth & development / Q000378:metabolism; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D008168:Lung / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D021382:Protein Sorting Signals; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics*; D014018:Tissue Distribution; D000097905:Roundabout Proteins
D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins