The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells.
A cDNA encoding a protein, NtrbohD, located on the plasma membrane and homologue to the flavocytochrome of the neutrophil NADPH oxidase, was cloned in tobacco. The corresponding mRNA was accumulated when tobacco leaves and cells were treated with the fungal elicitor cryptogein. After elicitation with cryptogein, tobacco cells transformed with antisense constructs of NtrbohD showed the same extracellular alkalinization as the control, but no longer produced active oxygen species (AOS). This work represents the first demonstration of the function of a homologue of gp91-phox in AOS production in elicited tobacco cells.
Laboratoire de Phytopharmacie et Biochimie des Interactions Cellulaires, UMR692 INRA/Université de Bourgogne, BP 86510, 21065 DIJON Cedex, France. simon@epoisses.inra.fr
Françoise
F
Simon-Plas
Taline
T
Elmayan
Jean-Pierre
JP
Blein
D020418:Algal Proteins / Q000494:pharmacology*; D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000737:chemistry*; D002478:Cells, Cultured; D005656:Fungal Proteins; D020869:Gene Expression Profiling; D017343:Genes, Plant / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013997:Time Factors; D014026:Nicotiana / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
D020418:Algal Proteins; D005656:Fungal Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C061032:cryptogein protein, Phytophthora cryptogea; D006861:Hydrogen Peroxide; D010088:Oxidoreductases