Differential effects of reduced glycoprotein VI levels on activation of murine platelets by glycoprotein VI ligands.
We have investigated the effects of decreased levels of the complex between glycoprotein VI (GPVI) and the Fc receptor gamma-chain (FcRgamma) on responses to collagen and GPVI-specific ligands in murine platelets. We show that levels of GPVI-FcRgamma of the order of 50% and 20% of wild-type levels caused 2- and 5-fold shifts to the right respectively in the dose-response curve for aggregation in response to collagen, the snake toxin convulxin and the monoclonal antibody JAQ1. In addition, there is a delay in the onset of aggregation in response to collagen. In contrast, the stimulation of protein tyrosine phosphorylation by collagen (as measured after 150 s) and adhesion to a collagen-coated surface under static conditions were unaffected in platelets with 50% and 20% of wild-type levels of GPVI. In contrast, responses to a collagen-related peptide (CRP), made up of repeat glycine-proline-hydroxyproline motifs, were markedly inhibited and abolished in platelets expressing 50% and 20% of wild-type levels of GPVI respectively. We suggest that the marked effect of a reduction in GPVI levels on the CRP-induced activation of platelets is due to the multivalent nature of CRP and the fact that GPVI is its sole receptor on platelets. Thus it appears that the interaction of CRP with GPVI is determined by a combination of affinity and avidity. The observation that collagen does not behave like CRP in platelets expressing reduced levels of GPVI, even in the combined presence of blocking antibodies against integrin alpha2beta1 and GPV, suggests that collagen has a greater affinity than CRP for GPVI, and/or that other receptors are involved in its binding to platelets. The clinical significance of these results is discussed.
Snell
Daniel C
DC
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, U.K.
Schulte
Valerie
V
Jarvis
Gavin E
GE
Arase
Kanako
K
Sakurai
Daiju
D
Saito
Takashi
T
Watson
Steve P
SP
Nieswandt
Bernhard
B
D000818:Animals; D000906:Antibodies / Q000276:immunology; D003094:Collagen / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D015539:Platelet Activation / Q000502:physiology*; D010980:Platelet Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D038981:Receptors, Collagen / Q000276:immunology; D017452:Receptors, IgG / Q000737:chemistry / Q000378:metabolism*
D000906:Antibodies; D010980:Platelet Membrane Glycoproteins; D038981:Receptors, Collagen; D017452:Receptors, IgG; C098341:platelet membrane glycoprotein VI; D003094:Collagen