Identification of genes differentially expressed between gastric cancers and normal gastric mucosa with cDNA microarrays.
To identify genes whose alterations lead to gastric cancer, gene expression profiles have been obtained from 22 gastric cancer tissues and their surrounding gastric mucosa tissues. A total of 16 genes were differentially expressed in more than 50% of gastric cancer tissues compared with surrounding gastric mucosa tissues. Genes such as HMG-Y, fibroblast collagenase inhibitor, and osteopontin are among those that are overexpressed in over 50% of the gastric cancer tissues. Dihydrodiol dehydrogenase, ribonuclease A, and glutathione peroxidase are among those genes that are underexpressed in over 50% of the gastric cancer tissues. We identified genes that are associated with clinical phenotypes of patients with gastric cancers. Alpha-II spectrin, Na/K-ATPase and KIAA0111 are those that are enhanced in intestinal type of gastric cancer. Gene such as platelet-endothelial tetraspan antigen 3 was enhanced in highly metastatic gastric cancer tissues.
In2Gen Company, Seoul National University Cancer Research Center, 6F, 28, Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
Seongeun
S
Lee
Myungin
M
Baek
Hankwang
H
Yang
Yung-Jue
YJ
Bang
Woo Ho
WH
Kim
Ji-Hong
JH
Ha
Dae-Kee
DK
Kim
Doo-Il
DI
Jeoung
D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D005979:Glutathione Peroxidase / Q000235:genetics; D025741:HMGA1a Protein / Q000235:genetics; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D053495:Osteopontin; D010088:Oxidoreductases / Q000235:genetics; D010641:Phenotype; D012044:Regression Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012259:Ribonuclease, Pancreatic / Q000235:genetics; D012795:Sialoglycoproteins / Q000235:genetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics; D013049:Spectrin / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery
D017931:DNA Primers; D018076:DNA, Complementary; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin; D025741:HMGA1a Protein; D013049:Spectrin; D010088:Oxidoreductases; D005979:Glutathione Peroxidase; C048338:trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; D012259:Ribonuclease, Pancreatic; D000254:Sodium-Potassium-Exchanging ATPase