Synthesis of nonluminescent lanthanide(III) chelates tethered to an aminooxy group and their applicability to biomolecule derivatization.
Synthesis of nonluminescent lanthanide(III) chelates tethered to an aminooxy group (i.e., 1-[4-(6-aminooxyhexamido)benzyl]diethylenetriaminetetraacetic acid lanthanides(III), 6a-d, where Ln(3+) is Eu, Dy, Sm, and Tb) is described. Their applicability to biomolecule derivatization is demonstrated by allowing them to react with a synthetic oligopeptide, a protein, two synthetic drugs, and a steroid. The oligopeptide and protein were linked to 6 after preoxidation of their N-terminal serine residues, while the drugs and the steroid reacted via their ketone functionality. Also some application data is included.
Peuralahti
Jari
J
PerkinElmer Life Sciences, Wallac Oy, P.O. Box 10, FIN-20101 Turku, Finland.
Puukka
Katri
K
Hakala
Harri
H
Mukkala
Veli-Matti
VM
Mulari
Outi
O
Hurskainen
Pertti
P
Hovinen
Jari
J
D000577:Amides / Q000737:chemistry; D000588:Amines; D002264:Carboxylic Acids; D002614:Chelating Agents / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D005453:Fluorescence; D006241:Haptens / Q000737:chemistry; D006801:Humans; D016209:Interleukin-8 / Q000737:chemistry; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry
D000577:Amides; D000588:Amines; D002264:Carboxylic Acids; D002614:Chelating Agents; D003432:Cross-Linking Reagents; D006241:Haptens; D016209:Interleukin-8; D028581:Lanthanoid Series Elements; D011506:Proteins