Characterisation of set-1, a conserved PR/SET domain gene in Caenorhabditis elegans.
The SET domain is a highly conserved domain shared between proteins of the antagonistic trithorax and Polycomb groups. It has been shown to play an important role in the assembly of either transcriptional activating or repressing protein complexes, and possesses a histone methyl-transferase activity. We report here the characterisation of the Caenorhabditis elegans gene, set-1, encoding a conserved SET-domain protein. We have analysed the developmental expression pattern of set-1 and show that maximal expression is observed early in development when set-1 is ubiquitously expressed. Its expression is more and more restricted as development progress. Gene inactivation by RNA interference shows that set-1 is an essential gene. Functional analysis of set-1 may contribute to the understanding of the molecular role of the SET domain.
Lymphocyte Development Group, MRC Clinical Sciences Centre, RPMS and Hammersmith Hospital, Du Cane Road, London W12 ONN, UK. remi.terranova@csc.mrc.ac.uk
Rémi
R
Terranova
Nathalie
N
Pujol
Laurent
L
Fasano
Malek
M
Djabali
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D017238:Genes, Helminth / Q000235:genetics; D049452:Green Fluorescent Proteins; D011495:Histone-Lysine N-Methyltransferase; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008780:Methyltransferases / Q000235:genetics*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000008:administration & dosage / Q000235:genetics; D018510:RNA, Helminth / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary; D008164:Luminescent Proteins; D012330:RNA, Double-Stranded; D018510:RNA, Helminth; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D008780:Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; C464501:Set-1 protein, C elegans