An in vitro study on the kinetics, subcellular distribution and phototoxicity of harmine in human tumor cells.
The beta-carboline alkaloid harmine, which was found to possess interesting phototoxic properties in different biological systems, was investigated for photokilling of human tumor cells in vitro. Harmine was readily accumulated by HeLa cells, localized preferably in cytoplasm, being a non toxic compound when used at low concentrations. The photoactivation of harmine-loaded cells with UV radiation showed lysosomal damage, reflected by altered localization of the fluorescent probe acridine orange, as well as an evident cell killing which increase with time up to a maximal value 48 h after the photodynamic treatment.
Hazen
M J
MJ
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Pérez-Gorroño
A I
AI
Ortiz-Movilla
N
N
Molero
M L
ML
D017484:Dermatitis, Phototoxic / Q000473:pathology*; D006247:Harmine / Q000493:pharmacokinetics* / Q000633:toxicity*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D011838:Radiation-Sensitizing Agents; D013347:Subcellular Fractions / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D011838:Radiation-Sensitizing Agents; D006247:Harmine