Rev-erbalpha gene expression in the mouse brain with special emphasis on its circadian profiles in the suprachiasmatic nucleus.
Rev-erbalpha is an orphan nuclear receptor that constitutively suppresses gene transcription. In the present study, the expression of Rev-erbalpha was investigated in the mouse brain by in situ hybridization using antisense cRNA probe. Positive Rev-erbalpha mRNA signals were detected widely in the brain with the highest expression in the suprachiasmatic nucleus (SCN). In the constant dark condition, the circadian expression profiles of Rev-erbalpha m RNA in the SCN showed a peak at early daytime (CT4) and a trough at early night time (CT16). The environmental lighting condition (light-dark environmental condition and exposure in the subjective night) did not alter the expression profiles. These findings indicate that Rev-erbalpha gene is a transcription factor intimately related to the circadian clock in the SCN.
Division of Molecular Brain Science, Department of Brain Sciences, Kobe University Graduate School of Medicine, Kobe, Japan.
Hiroyasu
H
Onishi
Shun
S
Yamaguchi
Kazuhiro
K
Yagita
Yoshiki
Y
Ishida
Xin
X
Dong
Hidehito
H
Kimura
Zhang
Z
Jing
Hidefumi
H
Ohara
Hitoshi
H
Okamura
D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D004268:DNA-Binding Proteins; D015870:Gene Expression / Q000502:physiology; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D018160:Receptors, Cytoplasmic and Nuclear; D013493:Suprachiasmatic Nucleus / Q000502:physiology*
D004268:DNA-Binding Proteins; C498262:Nr1d1 protein, mouse; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1; D011506:Proteins; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear