Determination of losartan and hydrochlorothiazide in tablets by CE and CEC.
Capillary Electrophoresis (CE) and Capillary Electrochromatography (CEC) have been used to determine losartan and hydrochlorothiazide. The CE separation was carried out in an uncoated capillary filled with a 100 mM sodium borate pH 9 solution containing trimethyl-beta-cyclodextrins. CEC was performed using a capillary packed with a RP-18 stationary phase. The mobile phase was a mixture of 50 mM ammonium acetate pH 7, water, acetonitrile (1/1.5/7.5). By CE and CEC suitable methods to determine simultaneously losartan and hydrochlorothiazide in working standard mixture or pharmaceutical form were obtained. The proposed methods are very simple and both gave accurate and precise results.
Dipartimento Studi Farmaceutici, Università degli Studi di Roma, La Sapienza, Piazzale Aldo Moro 5, Rome, Italy. mariagiovanna.quaglia@uniroma1.it
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D000959:Antihypertensive Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D006852:Hydrochlorothiazide / Q000032:analysis*; D007202:Indicators and Reagents; D019808:Losartan / Q000032:analysis*; D013607:Tablets
D000959:Antihypertensive Agents; D007202:Indicators and Reagents; D013607:Tablets; D006852:Hydrochlorothiazide; D019808:Losartan