Comparative analysis of commercial assays for the detection and quantification of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in plasma from patients infected with HIV-1 subtype C.
The commercial assays commonly used to quantify plasma human immunodeficiency virus type 1 (HIV-1) RNA in clinical settings were designed to assess HIV-1 subtype B. We compared the performance of 4 commercial assays (Amplicor versions 1.0 and 1.5 [Roche]; Quantiplex [Chiron]; and NASBA HIV-1 RNA QT [Organon Teknika]) in detecting and quantifying HIV-1 RNA in plasma from HIV-infected persons from Zambia, an area where HIV-1 subtype C is predominant. Each assay detected plasma HIV-1 RNA, but they do not all measure statistically similar quantities of plasma HIV-1 RNA.
Hoesley
Craig J
CJ
Department of Medicine, University of Alabama at Birmingham School of Medicine, Birmingham, AL, 35294, USA. choesley@uab.edu
Allen
Susan A
SA
Raper
James L
JL
Musonda
R
R
Niu
Yafen
Y
Gao
Feng
F
Squires
Kathleen E
KE
Aldrovandi
Grace M
GM
D005260:Female; D015658:HIV Infections / Q000097:blood*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008297:Male; D012367:RNA, Viral / Q000097:blood*; D019562:Viral Load
D012367:RNA, Viral