Fibroin-like substance is a major component of the outer layer of fertilization envelope via which carp egg adheres to the substratum.
Seven cDNA encoding silkworm fibroin homologues were cloned from a carp ovarian cDNA library. The encoded proteins are denoted as carp ovarian fibroin-like substances (FLS). FLS contain a repetitive domain consisting of tandem repeats of dipeptide of Gly-X, where X may be any amino acid. Each FLS has its own unique repeating sequence, such as GQGAGQGS, GQGMGQGM, GRGQGEGHGS, and GFGFGQGS, indicating a family of FLS genes exists in carp. FLS is exclusively expressed in oocytes and is stored in cortical granules. During cortical reaction, FLS is exocytosed to perivitelline space and then gradually added to the outer layer of the fertilization envelope (FEo). The FLS of fertilization envelope is conjugated with cystatin and cathepsin-like substance (CLS) and appears in multiple bands of molecular weights ranging from 40 to 205 kDa. After fertilization or artificial activation, carp eggs adhere firmly to the substratum via FEo. FLS is a major component of FEo. The presence of transglutaminase inhibitor, cadaverine or ethylene diaminetetraacetic acid, in the cortical reaction medium can impair or block the recruitment of FLS and other substances to FEo. As a consequence, FEo is not formed or is greatly reduced, resulting in a great reduction of egg adhesion.
Chang
Yea-Sha
YS
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
Huang
Fore-Lien
FL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000378:metabolism*; D018076:DNA, Complementary; D005089:Exocytosis; D005306:Fertilization; D005349:Fibroins / Q000032:analysis*; D022001:Focal Adhesions / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010063:Ovum / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment; D011503:Transglutaminases / Q000378:metabolism
D018076:DNA, Complementary; D005349:Fibroins; D011503:Transglutaminases