Determination of vitamin A palmitate in buccal mucosal cells: a pilot study.
We report in our present pilot study on the uptake of retinyl palmitate and its formation to retinol in buccal mucosal cells. Retinoids are able to change metaplastic lesions in several tissues. Prior to any clinical evaluation it is necessary to measure its uptake in target tissues such as the buccal mucosal epithelium. In this pilot study 12 volunteers creamed the inside of their cheeks lightly with a 0.1% retinyl palmitate containing toothpaste for 10 days followed by a wash out phase. On day 0, 3, 7, 10, 17 and 21, buccal mucosal cell samples were taken from volunteers and the uptake of retinol and retinyl palmitate was determined by HPLC analysis. An uptake of vitamin A was demonstrated in all volunteers. Comparing day 0 to day 3 a significant uptake of retinyl palmitate (p<0.05) was detected and comparing day 3 to 17 and 21 a significant decrease (p<0.05) during growth and differentiation of the mucosal epithelium could be seen. The subsequently formed retinol showed an increase but no statistical significance was determined. - The uptake of retinyl palmitate and the formation to retinol led to an enrichment of vitamin A in buccal mucosal cells. In this way metaplastic alterations of the buccal mucosal epithelium might be prevented or reversed by the application of topical retinyl palmitate.
University of Hohenheim, Department of Biological Chemistry and Nutrition, Fruwirthstr. 12, D-70593 Stuttgart, Germany.
U
U
Sobeck
A
A
Fischer
H K
HK
Biesalski
D000328:Adult; D004247:DNA / Q000032:analysis; D004224:Diterpenes; D005260:Female; D006801:Humans; D008297:Male; D009061:Mouth Mucosa / Q000737:chemistry / Q000378:metabolism*; D009910:Oral Hygiene; D010865:Pilot Projects; D000084562:Retinyl Esters; D014100:Toothpastes; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics*
D004224:Diterpenes; D000084562:Retinyl Esters; D014100:Toothpastes; D014801:Vitamin A; C014794:retinol palmitate; D004247:DNA