[Quantity and apoptosis-related protein level of B lymphocyte in patients with immunorelated pancytopenia].
OBJECTIVE
To examine the quantity and apoptosis-related protein level of B lymphocyte in the patients with immunorelated pancytopenia (IRP) and explore the role of B lymphocyte in the pathogenetic mechanism of IRP.


METHODS
Quantities of all B lymphocytes and CD(5)(+) B lymphocytes and the expressions of Fas and bcl-2 on B lymphocytes in 25 patients with untreated IRP, 15 IRP patients in complete remission (CR) and 10 normal controls were assayed by FACS.


RESULTS
The percentages of B lymphocyte and CD(5)(+) B lymphocytes were significantly higher in untreated IRP patients than in CR IRP patients and normal controls (P < 0.05); there was no significant difference between the latter two groups (P > 0.05). There was no significant difference of Fas expression in B lymphocytes among the three groups (P > 0.05). The expression of bcl-2 on B lymphocytes was significantly higher in untreated patients than in CR patients or normal controls (P < 0.05), and so did in CR patients than in normal controls (P < 0.01). The apoptosis-related index was significantly lower in untreated patients than in CR patients or normal controls (P < 0.01), and was lower in CR patients than in normal controls (P < 0.05). The percentage of B lymphocyte was positively correlated with the duration from the beginning of treatment to response.


CONCLUSIONS
The production of auto-antibodies in IRP patients probably has some relationships with the abnormal quantities of B lymphocyte and its subsets, and with the inhibition of B lymphocyte apoptosis.
Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
Rong
R
Fu
Zonghong
Z
Shao
Hong
H
He
Hong
H
Liu
Hairong
H
Jia
Juan
J
Sun
Mingfeng
M
Zhao
Guangsheng
G
He
Jun
J
Shi
Jie
J
Bai
Yulin
Y
Chu
Tianying
T
Yang
D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000502:physiology; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology / Q000473:pathology*; D001853:Bone Marrow / Q000503:physiopathology*; D018956:CD5 Antigens / Q000276:immunology; D002452:Cell Count; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007154:Immune System Diseases / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D010198:Pancytopenia / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis* / Q000378:metabolism; D019014:fas Receptor / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D018956:CD5 Antigens; D019253:Proto-Oncogene Proteins c-bcl-2; D019014:fas Receptor