A partially 3-O-methylated (1-->4)-linked alpha-D-galactan and alpha-D-mannan from Pleurotus ostreatoroseus Sing.
The two main water-soluble extracellular polysaccharides produced by the basidiomycete fungus Pleurotus ostreatoroseus Sing were isolated and purified. They were characterized using 13C, 1H, and 1H, 13C HMQC NMR spectroscopy, methylation analysis, and Smith degradation. One was a mannan having a main chain of (1-->6)-linked alpha-D-mannopyranosyl residues, almost all of which were branched at O-2 with side chains of different lengths, containing 2-O- and 3-O-linked mannopyranosyl units. The other was a partially 3-O-methylated (1-->4)-linked alpha-D-galactopyranan, a structure that has not been previously described.
Rosado
Fábio R
FR
Departamento de Bioquímica, Universidade Estadual de Maringá, Av. Colombo 5790, Brazil.
Carbonero
Elaine R
ER
Kemmelmeier
Carlos
C
Tischer
Cesar A
CA
Gorin
Philip A J
PA
Iacomini
Marcello
M
D005685:Galactans / Q000032:analysis* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000032:analysis* / Q000378:metabolism; D008745:Methylation; D020076:Pleurotus / Q000737:chemistry* / Q000378:metabolism; D014867:Water / Q000737:chemistry
D005685:Galactans; D008351:Mannans; D014867:Water