Alterations in the mouse and human proteome caused by Huntington's disease.
Huntington's disease is an autosomal dominantly inherited disease that usually starts in midlife and inevitably leads to death. In our effort to identify proteins involved in processes upstream or downstream of the disease-causing huntingtin, we studied the proteome of a well established mouse model by large gel two-dimensional electrophoresis. We could demonstrate for the first time at the protein level that alpha1-antitrypsin and alphaB-crystalline both decrease in expression over the course of disease. Importantly, the alpha1-antitrypsin decrease in the brain precedes that in liver and testes in mice. Reduced expression of the serine protease inhibitors alpha1-antitrypsin and contraspin was found in liver, heart, and testes close to terminal disease. Decreased expression of the chaperone alphaB-crystallin was found exclusively in the brain. In three brain regions obtained post-mortem from Huntington's disease patients, alpha1-antitrypsin expression was also altered. Reduced expression of the major urinary proteins not found in the brain was seen in the liver of affected mice, demonstrating that the disease exerts its influence outside the brain of transgenic mice at the protein level. Maintaining alpha1-antitrypsin and alphaB-crystallin availability during the course of Huntington's disease might prevent neuronal cell death and therefore could be useful in delaying the disease progression.
Zabel
Claus
C
Institut für Humangenetik, Universitätsklinikum Charité, 13353 Berlin, Germany.
Chamrad
Daniel C
DC
Priller
Josef
J
Woodman
Ben
B
Meyer
Helmut E
HE
Bates
Gillian P
GP
Klose
Joachim
J
D000818:Animals; D001921:Brain / Q000378:metabolism; D003433:Crosses, Genetic; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D006816:Huntington Disease / Q000235:genetics* / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013737:Testis / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis* / Q000737:chemistry; D038203:alpha-Crystallin B Chain / Q000096:biosynthesis* / Q000737:chemistry
D020543:Proteome; D000515:alpha 1-Antitrypsin; D038203:alpha-Crystallin B Chain