Chemical stress-responsive genes from the lignin-degrading fungus Phanerochaete chrysosporium exposed to dibenzo-p-dioxin.
The stress-responsive genes expressed against the exogenous addition of dibenzo-p-dioxin from the lignin-degrading basidiomycete, Phanerochaete chrysosporium, were determined utilizing a differential display reverse transcription-PCR technique. Six cDNA fragments, exhibiting a high homology with various proteins from other microorganisms, were identified via a BLAST search; that is, NADH-ubiquinone oxidoreductase (NUO), ATP/ADP carrier, uric acid-xanthine permease, manganese superoxide dismutase, 3-hydroxybutyryl-coenzyme A dehydrogenase, and cytoskeletal protein. The expression of NUO was also up-regulated by catechol and trihydroxybenzene but not by dibenzofuran, suggesting that NUO expression was initiated by the formation of quinone products through the reaction of extracellular one-electron oxidizing enzymes with dibenzo-p-dioxin.
Kurihara
Hiroyuki
H
Faculty of Agriculture, Kyushu University, 6-10-1, Hakokzaki, Higashi-ku, Fukuoka 812-8581, Japan.
Wariishi
Hiroyuki
H
Tanaka
Hiroo
H
D004147:Dioxins / Q000633:toxicity*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006706:Homeostasis / Q000502:physiology; D008031:Lignin / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D020075:Phanerochaete / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism
D004147:Dioxins; D008031:Lignin; C020887:dibenzo(1,4)dioxin