Oleamide attenuates apoptotic death in cultured rat cerebellar granule neurons.
The effect of oleamide on apoptosis was investigated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assay, DNA staining assay with propidium iodide and caspase-3 activity analyses. The present results showed that oleamide significantly attenuated the cell death and nuclear condensation of cultured rat cerebellar granule neurons induced by K(+) deprivation in a dose-dependent manner. The oleamide actions were well parallel with the attenuation of caspase-3 activity in the process of apoptotic death. Moreover, neither elaidic acid nor stearic acid, two fatty acids structurally related to oleamide without the Delta(9)-cis double bond, had similar effects on the cell death, suggesting the selectively structural features of oleamide required for this action. These data provided the first evidence of a protective effect of oleamide against apoptosis in a structurally specific manner.
Department of Pharmacology, Shenyang Pharmaceutical University, 110016, Shenyang, PR China.
Jing-Yu
JY
Yang
Kazuho
K
Abe
Nan-Jie
NJ
Xu
Norio
N
Matsuki
Chun-Fu
CF
Wu
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D053148:Caspase 3; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002525:Cerebellar Cortex / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000378:metabolism* / Q000494:pharmacology; D009829:Oleic Acids / Q000378:metabolism* / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D011191:Potassium Deficiency / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D061945:Caspase Inhibitors; D018696:Neuroprotective Agents; D009829:Oleic Acids; C029407:oleylamide; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; D011188:Potassium