Determination of thyroid hormones in pharmaceutical preparations, after derivatization with 9-anthroylnitrile, by high-performance liquid chromatography with fluorescence detection.
HPLC with fluorescence detection was used for the determination of low levels of liothyronine sodium and levothyroxine sodium in pharmaceutical preparations after fluorogenic derivatization. 9-Anthroylnitrile in dimethyl sulfoxide was used as a precolumn fluorogenic reagent. The 9-anthroylnitrile derivatives of liothyronine sodium and levothyroxine sodium were separated on a reversed-phase column with acetonitrile-0.02 M sodium dodecylsulfate (pH 3.5 with phosphoric acid) as the eluent. The calibration graphs were linear over a sample concentration range of 0.25-2.5 microg/ml. The detection limits for liothyronine sodium and levothyroxine sodium were 0.2 ng per injection. The proposed method was applied to the determination of thyroid hormones in pharmaceutical preparations.
Tokyo Metropolitan Research Laboratory of Public Health, Japan. misataka@tokyo-eiken.go.jp
Misako
M
Takahashi
Machiko
M
Nagashima
Sutemi
S
Shigeoka
Hisashi
H
Kamimura
Kunihiro
K
Kamata
D000873:Anthracenes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007202:Indicators and Reagents / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013963:Thyroid Hormones / Q000032:analysis*
D000873:Anthracenes; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D013963:Thyroid Hormones; C066291:9-anthroylnitrile