Increases in amino-cupric-silver staining of the supraoptic nucleus after sleep deprivation.
Sleep deprived rats undergo a predictable sequence of physiological changes, including changes in skin condition, increased energy expenditure, and altered thermoregulation. Amino-cupric-silver staining was used to identify sleep deprivation related changes in the brain. A significant increase in staining was observed in the supraoptic nucleus (SON) of the hypothalamus of rats with high sleep loss (>45 h) vs. their yoked controls. Follow-up experiments showed that staining was not significantly different in rats sleep deprived for less than 45 h, suggesting that injurious sleep deprivation-related processes occur above a threshold quantity of sleep loss. These anatomical changes suggest that the effects of sleep deprivation may be related to protein metabolism in certain brain regions.
University of California, Los Angeles, CA, USA.
Monica M
MM
Eiland
Lalini
L
Ramanathan
Seema
S
Gulyani
Marcia
M
Gilliland
Bernard M
BM
Bergmann
Allan
A
Rechtschaffen
Jerome M
JM
Siegel
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012892:Sleep Deprivation / Q000378:metabolism*; D013194:Staining and Labeling; D013495:Supraoptic Nucleus / Q000378:metabolism*; D013997:Time Factors