Novel Sxr(a) ES cell line offers hope for Y chromosome gene-targeted mice.
A mouse targeted for a Y Chromosome gene has not been reported. Because the Y Chromosome is present in only one copy, and most of its genes are critical for germ cell development, such a mouse would likely be infertile. Thus, we describe a new reproductive strategy to enable transmission of targeted Y Chromosome genes to subsequent generations. The strategy uses two segregating copies of Y Chromosome genes to mimic the autosomal condition. To achieve this, we developed a new embryonic stem cell line from the XYSxr(a) mouse, which carries a duplication of the gene-rich Y Chromosome short arm. Importantly, we demonstrate germ line transmission of the YSxr(a) chromosome and describe this significant new tool as a practical solution to enable reproduction in mice targeted for Y Chromosome genes.
Centre for Molecular Medicine and Therapeutics, British Columbia Research Institute for Children's and Women's Health, and Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada. simpson@cmmt.ubc.ca
Elizabeth M
EM
Simpson
Kevin A
KA
Johnson
Bobbi-Jo
BJ
Shirley
Ge Y
GY
Fang
Janet K
JK
Bayleran
Charles P
CP
Lerner
D000818:Animals; D002460:Cell Line; D020440:Gene Duplication; D018390:Gene Targeting; D007178:Inbreeding; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013234:Stem Cells; D014998:Y Chromosome