Purification and Functional Characterization of a Shark Cartilage Factor Inhibitory to Angiogenesis.
SCAIF-I, an inhibitor of angiogenesis from shark cartilage was purified to homogeneity. The 4 mol/L guanidinium chloride extract of shark cartilage was fractionally precipitated with 35%-65% acetone, then purified by Resource Q ion exchange chromatography, Sephacryl S-300 gel filtration, and reverse-phase high performance liquid chromatography. The pure inhibitor was homogeneous as a single band on a silver-stained 15% sodium dodecyl sulfate-polyacrylamide gel. SCAIF-I had an molecular weight of 18 kD. It specifically inhibited proliferation of endothelial cells, and strongly blocked endothelial cell movement and angiogenesis in the chorioallantoic membrane of chick embryos. Systemic administration of SCAIF-I at the dose of 5 mg/kg.d suppressed 87.93% of the growth of Lewis lung carcinoma implanted in C57BL/6 mice.
Shen
Xian-Rong
XR
Naval Medical Research Institute, Shanghai 200433, China. 970240@fudan.edu.cn
Ji
Dong-Mei
DM
Jia
Fu-Xing
FX
Deng
Xin-Xian
XX
Sun
Jia-Hong
JH
Hu
Xing-Ran
XR
Ren
Da-Ming
DM