A sieving method for collecting the metacercariae of trematode parasites from freshwater fish.
This study describes a sieving method for the collection of metacercariae from frozen (-20 degrees C) freshwater fish. Digested fish tissue is filtered through a series of sieves; the crude filtrate is then centrifuged. Centrifugation produces a sediment from which metacercariae can be removed. Half of the metracercariae that were obtained from the fish meat that had been frozen for 10 days (-20 degrees C) were dead; the other half were alive and some larvae were moving slowly.
Institute and Department of Parasitology, National Yangming University, Taipei, Taiwan, Republic of China. pcfan@ym.edu.tw
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PC
Fan
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Wu
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Huang
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Yen
D000818:Animals; D002498:Centrifugation; D005399:Fishes / Q000469:parasitology*; D005618:Fresh Water / Q000469:parasitology; D010270:Parasite Egg Count / Q000379:methods*; D013624:Taiwan; D014200:Trematoda / Q000254:growth & development / Q000302:isolation & purification*