Single-tube genotyping of MBL-2 polymorphisms using melting temperature analysis.
Recently several authors correlated MBL-2 gene polymorphisms with different pathologies and there is a growing interest for MBL-2 genotyping in a large number of individuals. We have developed a single-tube, rapid, economic, and fully automated melting temperature analysis screening method, based on ABI 7700 Sequence Detection System technology and SYBR Green I chemistry, for the detection of three polymorphisms (exon 1, codons 52, 54, 57) in the MBL-2 gene. We also developed an electronic sheet for the automatic calling of different genotypes, based on the analysis of the first derivative of ABI 7700 raw data.
Dipartimento di Scienze della Riproduzione e dello Sviluppo, Università di Trieste, Italy.
U
U
Hladnik
L
L
Braida
M
M
Boniotto
D
D
Pirulli
F
F
Gerin
A
A
Amoroso
S
S
Crovella
D002352:Carrier Proteins / Q000235:genetics*; D005821:Genetic Techniques; D005838:Genotype; D006801:Humans; D037241:Mannose-Binding Lectins; D011110:Polymorphism, Genetic; D013696:Temperature
D002352:Carrier Proteins; D037241:Mannose-Binding Lectins