[The detection of cyanobacterial cells by a non-fluorescent in situ hybridization method].
Seven cyanobacterial strains (Anabaena macrospora NIER10016, Oscillatoria sp. NIER10042, Microcystis aeruginosa NIER10015, M. ichtyoblabe NIER10025 and NIER10040, M. novacekii NIER10029, and M. wesenbergii NIER10068) were tested by a non-fluorescent in situ hybridization method using two specific horseradish peroxidase--labeled oligonucleotide probes and two chromogenic substrates. This approach was shown to be appropriate for analysis of natural samples.
Bel'kova
N L
NL
Limnological Institute, Siberian Division, Russian Academy of Sciences, ul. Ulan-Batorskaya 3, Irkutsk, 664033 Russia. belkova@lin.irk.ru
Chung
H M
HM
Ahn
T S
TS
D000458:Cyanobacteria / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D006735:Horseradish Peroxidase; D017403:In Situ Hybridization / Q000379:methods; D015345:Oligonucleotide Probes
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D006735:Horseradish Peroxidase