[Association between Helicobacter pylori cagA strain infection and expression of cyclooxygenase 2 in gastric carcinoma].
OBJECTIVE
To observe the expression of cyclooxygenase 2 (COX-2) in tissues of human gastric cancer and explore the association between Helicobacter pylori cagA strain infection and the expression of COX-2 so as to provide a theoretical basis for early prevention of gastric cancer.


METHODS
Flow cytometry was adopted to quantitatively determine and analyze the expression of COX-2 in 31 specimens of gastric cancer and normal juxta cancerous tissues. PCR was used to detect the cagA gene in gastric cancer.


RESULTS
COX-2 was over-expressed in 26 of the 31 (84%) specimens of gastric cancer. The expression of COX-2 in the 18 specimens of gastric cancer with cagA positive strain infection was significantly higher than that in the 13 specimens with cagA negative strain infection.


CONCLUSIONS
COX-2 is over-expressed in gastric cancer and cagA positive strain infection up-regulates the expression of COX-2 in gastric cancer. There may be another way or channel to regulate the expression of COX-2 in gastric cancer besides cagA positive strain infection. Therefore, applying COX-2 selective inhibitors to prevent gastric cancer can be an effective and promising way.
Cancer Institute, The First Clinical College, China Medical University, Shenyang 110001, China.
Xiaolin
X
Guo
Li'e
L
Wang
Yuan
Y
Yuan
D000368:Aged; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics*; D051546:Cyclooxygenase 2; D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors / Q000627:therapeutic use; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000382:microbiology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors; D007527:Isoenzymes; D008565:Membrane Proteins; C080866:cagA protein, Helicobacter pylori; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases