Immunochemical and immunocytochemical expression of protein kinase c isoenzymes alpha, delta, epsilon and zeta in primary adherent cultures of chick chondrocytes.
The family of protein kinase C (PKC) comprises serine/threonine isoenzymes involved in various biological processes, including cell proliferation and differentiation. On the bases of previous investigations performed by us on the expression of various PKC isoforms in the endochondral ossification process of the vertebral column, the aim of the present work was to investigate the expression of various PKC-isoenzymes in chick primary chondrocyte cultures i.e. the most used chondrocyte culture model in vitro. Immunochemical and immunocytochemical experiments were performed to detect the expression of PKC-alpha, -delta, -epsilon and -zeta. Chondrocyte cultures were examined two weeks after cell collection from tibiae of 6-day old chick embryos. By means of morphological observations associated with the immunocytochemical expression of type II collagen, two different cell phenotypes were identified, i.e. fibroblast-like and polygonal-roundish-shaped cells. As far as PKC-isoenzyme expression was concerned, PKC-zeta revealed a stronger immunochemical and immunocytochemical expression; PKC-alpha exhibited a positivity less marked than PKC-zeta, whereas PKC-delta and -epsilon were less expressed in this culture stage. It is reasonable that a major role could be played by PKC-alpha and -zeta in this phase of the chondrogenic process, whereas PKC-delta and -epsilon could be involved in different stages of chondrocyte differentiation in vitro.
Department of Human Morphology, University of Trieste, Italy. grill@units.it
Vittorio
V
Grill
Maria A
MA
Sandrucci
Marisa
M
Basa
Vanessa
V
Nicolin
Paola
P
Narducci
Renato
R
Bareggi
Alberto M
AM
Martelli
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D015153:Blotting, Western; D001842:Bone and Bones / Q000166:cytology / Q000196:embryology* / Q000201:enzymology*; D002356:Cartilage / Q000166:cytology / Q000196:embryology* / Q000201:enzymology*; D002451:Cell Compartmentation / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D048429:Cell Size / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D019902:Chondrocytes / Q000166:cytology / Q000201:enzymology*; D024043:Collagen Type II / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D007527:Isoenzymes / Q000378:metabolism; D023421:Models, Animal; D011493:Protein Kinase C / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D000099221:Protein Kinase C zeta
D000906:Antibodies; D024043:Collagen Type II; D007527:Isoenzymes; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D000099221:Protein Kinase C zeta