Immunohistochemical detection of CD79a expression in precursor T cell lymphoblastic lymphoma/leukaemias.
Twenty-three cases of precursor T cell lymphoid malignancies were examined with respect to CD79a expression. Immunohistochemical staining was performed on frozen tissue sections using a broad panel of antibodies and Southern blot analysis was undertaken using DNA probes encoding immunoglobulin heavy chain (IgH) gene and T-cell receptor (TCR) beta, gamma and delta genes. Twelve (52%) of the 23 cases examined demonstrated CD79a expression. IgH and TCRbeta, gamma and delta gene rearrangements were found in 5, 9, 12 and 20 cases, respectively. CD79a-positive neoplastic cells exhibited a phenotype and genotype characteristic of an early stage of T cell differentiation. Immunohistochemical staining was also performed on human thymus and thymoma to investigate the normality of CD79a expression, to discover that low-level expression of CD79a is common in thymocytes and thymoma-associated lymphocytes. These results suggest that CD79a is expressed weakly and transiently in immature T-lineage cells.
Pathology Section, Nagoya University Hospital, Japan.
Mitsuyoshi
M
Hashimoto
Yoriko
Y
Yamashita
Naoyoshi
N
Mori
D015703:Antigens, CD / Q000032:analysis*; D015139:Blotting, Southern; D051925:CD79 Antigens; D016009:Chi-Square Distribution; D005260:Female; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D007150:Immunohistochemistry; D007223:Infant; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000276:immunology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D013950:Thymus Gland / Q000276:immunology
D015703:Antigens, CD; D051925:CD79 Antigens; C497881:CD79A protein, human; D007143:Immunoglobulin Heavy Chains; D011947:Receptors, Antigen, B-Cell