Zinc accelerates dengue virus type 2-induced apoptosis in Vero cells.
Dengue virus type 2 (DENV-2) infection induced apoptotic cellular DNA fragmentation in Vero cells within 8 days of infection. The addition of high concentrations of extracellular Zn(2+) but not Ca(2+), Mg(2+) or Mn(2+) to the cell culture medium hastened the detection of apoptosis to within 4 h after infection. No apoptotic cellular DNA fragmentation was detected in the cell culture treated with Zn(2+) alone or infected with heat- or ultraviolet light-inactivated DENV-2 in the presence of Zn(2+). These results suggest that (i) apoptosis is induced in African green monkey kidney cells infected with live DENV-2 and (ii) the addition of high extracellular Zn(2+) accelerates detection of apoptosis in the DENV-2-infected cells.
Department of Medical Microbiology, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia.
Norazizah
N
Shafee
Sazaly
S
AbuBakar
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology*; D002522:Chlorocebus aethiops; D003716:Dengue Virus / Q000502:physiology*; D020287:In Situ Nick-End Labeling; D014709:Vero Cells; D015032:Zinc / Q000494:pharmacology*
D015032:Zinc