Inhibition of retinoid activity by components of a paper mill effluent.
A cell line stably transfected with reporter genes activated by retinoic acid was used to test a paper mill effluent for the presence of retinoids or components that interfere with retinoic acid-stimulated gene transcription. No retinoids were detected in effluent or control water. However, effluent water significantly decreased reporter activity stimulated by all-trans-retinoic acid, while activity stimulated by 9-cis-retinoic acid was unaffected. In a limited fractionation through a C-18 solid phase-exchange column the inhibitory activity was retained in the aqueous fraction, indicating that the activity was polar.
Mid-Continent Ecology Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Duluth, Minnesota 55811, USA. pschoff@nrri.umn.edu
Patrick K
PK
Schoff
Gerald T
GT
Ankley
D000818:Animals; D002460:Cell Line; D017930:Genes, Reporter; D007220:Industrial Waste / Q000009:adverse effects*; D051379:Mice; D010209:Paper; D018168:Receptors, Retinoic Acid / Q000187:drug effects* / Q000235:genetics; D014158:Transcription, Genetic; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014873:Water Pollutants / Q000009:adverse effects*
D007220:Industrial Waste; D018168:Receptors, Retinoic Acid; D014873:Water Pollutants; D014212:Tretinoin