Retinoic acid differently affects the formation of palps and surrounding neurons in the ascidian tadpole.
The anterior-most surface of the ascidian tadpole larvae is composed of specialized complex structures, including adhesive organs (palps) and the surrounding sensory neurons (RTENs) connected to neurons inside the palps. These are derived from a-line blastomeres by inductive effects from A-line blastomeres. The induction is reported to coordinate the expression of homeobox genes in the anterior epidermis, which can be affected by all- trans retinoic acid (RA). RA treatment also results in failure of the morphological formation of palps. Here we first isolated a gene intensely expressed in the cells of the anterior structure from the time of their lineage restriction, and then found that the RA treatment did not affect the specific gene expression in the presumptive palp cells but did affect that in the RTENs. These results suggest that the palp formation involves at least two different processes, a RA-insensitive cell-type specification process and a RA-sensitive morphogenetic process. RA treatment also affects the morphogenetic process of the palp formation and also disturbs the precise patterning of the surrounding epidermis, which may contribute to the regulation of RTEN development.
Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Kasumi
K
Yagi
Kazuhiro W
KW
Makabe
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D017403:In Situ Hybridization; D007814:Larva / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D009474:Neurons / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D014561:Urochordata / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D014212:Tretinoin