Introduction of lanthanide(III) chelates to oligopeptides on solid phase.
The synthesis of oligopeptide building blocks for the introduction of nonluminescent and luminescent lanthanide(III) chelates to the oligopeptide structure on the solid phase is described. The oligopeptide conjugates synthesized were used in DELFIA-based receptor binding assay (motilin) as well as in LANCE time-resolved fluorescence quenching assay (caspase-3).
PerkinElmer Life Sciences, Wallac Oy, P.O. Box 10, FIN-20101 Turku, Finland.
Jari
J
Peuralahti
Harri
H
Hakala
Veli-Matti
VM
Mukkala
Kristiina
K
Loman
Pertti
P
Hurskainen
Outi
O
Mulari
Jari
J
Hovinen
D053148:Caspase 3; D020169:Caspases / Q000032:analysis; D002614:Chelating Agents / Q000138:chemical synthesis; D020650:Combinatorial Chemistry Techniques; D005453:Fluorescence; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008722:Methods; D009842:Oligopeptides / Q000138:chemical synthesis*; D011485:Protein Binding; D011964:Receptors, Gastrointestinal Hormone / Q000378:metabolism; D018013:Receptors, Neuropeptide / Q000378:metabolism
D002614:Chelating Agents; D028581:Lanthanoid Series Elements; D009842:Oligopeptides; D011964:Receptors, Gastrointestinal Hormone; D018013:Receptors, Neuropeptide; C050802:motilin receptor; D053148:Caspase 3; D020169:Caspases