[Photoquenching of the bioluminescence of the genetically engineered Escherichia coli TG1 (pXen7) strain in the presence of photodithazine].
The photoquenching of the bioluminescence of the genetically engineered Escherichia coli TG1 (pXen7) strain was studied in the presence of the photosensitizer photodithazine, a glucosamine salt of chlorin e6. The photosensitized quenching of the bioluminescence was found to correlate with the colony-forming ability of the strain. The data obtained are discussed from the standpoint of using biosensor luminescent bacterial systems for the assessment of the efficiency of photosensitizers in antimicrobial photochemotherapy.
Strakhovskaia
M G
MG
Department of Biophysics, Faculty of Biology, Moscow State University, Vorob'evy gory, Moscow, 119899 Russia. marstr@biophys.msu.ru
Parkhomenko
I M
IM
Rumbal'
Ia V
IaV
Zarubina
A P
AP
Danilov
V S
VS
Stranadko
E F
EF
D015374:Biosensing Techniques; D002735:Chlorophyllides; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D008027:Light; D008163:Luminescent Measurements; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D011166:Porphyrins / Q000737:chemistry / Q000494:pharmacology*; D011995:Recombination, Genetic
D002735:Chlorophyllides; D017319:Photosensitizing Agents; D011166:Porphyrins; C062985:phytochlorin; D005944:Glucosamine