Optimization of fusion PCR for in vitro construction of gene knockout fragments.
Oklahoma State University, Stillwater 74075, USA.
Hong-Liang
HL
Wang
Bradley L
BL
Postier
Robert L
RL
Burnap
D019677:Artificial Gene Fusion / Q000379:methods*; D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D015870:Gene Expression; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011786:Quality Control; D019914:Taq Polymerase / Q000235:genetics / Q000378:metabolism
D017931:DNA Primers; D019914:Taq Polymerase