Primary culture of porcine pancreatic acinar cells.
BACKGROUND
The methodology of acinar cell culture has become of primary importance in the research of pancreatic physiology and pharmacology.


OBJECTIVE
To develop a method for primary culture of porcine pancreatic acinar cells.


METHODS
Dispersed pancreatic acinar cells were made by RPMI-1640 medium containing collagenase III. After purification, the isolated acinar cells were cultured in RPMI-1640 medium with 2.5% fetal bovine serum. The morphologic characteristics of acinar cells were described. (3)H-thymidine incorporation of acinar cells and activity of amylase or lipase were determined during the culture.


RESULTS
There were no remarkable morphologic changes in the pancreatic acinar cells during the 20-day culture. The acini showed the tendency of gathering but did not attach to the walls of the culture disks. Incorporation of (3)H-thymidine in acinar cells in the primary culture was well kept. The secretion of amylase or lipase from acini decreased with the time of culture.


CONCLUSIONS
In the primary culture of acinar cells from porcine pancreas developed in this study, the acinar cells retained normal morphology and ability of growth but not secretion of amylase or lipase. The method would be beneficial for further experiments on acini of porcine pancreas.
Department of Gastroenterology, The First Hospital, Chongqing University of Medical Sciences, Chongqing, P.R. China. cwtang@public.cta.cq.cn
Chengwei
C
Tang
Xudong
X
Zhao
Jun
J
Han
D000681:Amylases / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D010179:Pancreas / Q000166:cytology* / Q000201:enzymology; D013552:Swine
D000681:Amylases