Functional genomic analysis of the Bacillus subtilis Tat pathway for protein secretion.
Protein secretion from Bacillus species is a major industrial production tool with a market of over $1 billion per year. However, standard export technologies, based on the well-characterised general secretory (Sec) pathway, are frequently inapplicable for the production of proteins. The recently discovered twin-arginine translocation (Tat) pathway offers additional potential to transport proteins. Here we review the use of functional genomic and proteomic approaches to explore the Tat pathway of Bacillus subtilis. The properties of Tat pathway components and the twin-arginine signal peptides that direct proteins into this pathway are discussed. Where appropriate, a comparison is made with Tat systems from other organism, such as Escherichia coli. Recent findings with the latter organism in particular provide proof-of-principle that the Tat pathway can be exploited for the production of Sec-incompatible proteins.
van Dijl
Jan Maarten
JM
Department of Pharmaceutical Biology, University of Groningen, A. Deusinglaan 1, The Netherlands. j.m.van.dijl@farm.rug.nl
Braun
Peter G
PG
Robinson
Colin
C
Quax
Wim J
WJ
Antelmann
Haike
H
Hecker
Michael
M
Müller
Jörg
J
Tjalsma
Harold
H
Bron
Sierd
S
Jongbloed
Jan D H
JD
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001692:Biological Transport; D030562:Databases, Protein; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D016680:Genome, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008956:Models, Chemical; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D021382:Protein Sorting Signals / Q000235:genetics; D021381:Protein Transport; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D021382:Protein Sorting Signals; C408585:twin-arginine translocase complex, E coli