Association study of the NRAMP1 gene promoter polymorphism and early-onset type 1 diabetes.
Natural resistance-associated macrophage protein 1 (NRAMP1) has an important role in regulating macrophage functions that affect innate resistance as well as immune responses. We analyzed the microsatellite polymorphism in the promoter region of the human NRAMP1 gene in 206 type 1 diabetes patients and 200 normal children to determine whether this polymorphism might be associated with type 1 diabetes in the Japanese population. The frequency of allele 2 (180 bp) of the promoter microsatellite polymorphism of the NRAMP1gene was slightly lower in the early-onset population (2-10 years of age) of type 1 diabetes patients than in controls, although the difference did not reach statistical significance. The association study of the cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) gene, located near the NRAMP1 gene, and type 1 diabetes showed that the CTLA-4 gene significantly contributed to the development of type 1 diabetes, whereas NRAMP1 had an additional effect on the onset of type 1 diabetes in the young population.
Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Wafaa M
WM
Bassuny
Kenji
K
Ihara
Nobuo
N
Matsuura
Saifuddin
S
Ahmed
Hitoshi
H
Kohno
Ryuichi
R
Kuromaru
Kenichi
K
Miyako
Toshiro
T
Hara
D000069594:Abatacept; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000235:genetics; D060908:CTLA-4 Antigen; D027682:Cation Transport Proteins / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D018796:Immunoconjugates; D007223:Infant; D007231:Infant, Newborn; D007564:Japan; D015810:Linkage Disequilibrium; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D000099173:Natural Resistance-associated Macrophage Protein 1
D000069594:Abatacept; D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; D027682:Cation Transport Proteins; D018796:Immunoconjugates; D000099173:Natural Resistance-associated Macrophage Protein 1; C556706:CTLA4 protein, human