Restriction fragment alleles of the rabbit IGHG genes with reference to the rabbit IGHGCH2 or e locus polymorphism.
Among domesticated mammals, rabbit (Oryctolagus cuniculus) is the only species possessing not more than one subclass of immunoglobulin (IgG) antibodies. The rabbit IGHGCH2 or e locus presents two serologically defined alleles, the e14 and e15 allotypes, which are correlated with amino acid variation at the IgG CH2-CH3 interface. Genetic studies, while revealing the adaptive value of this polymorphism, have relied so far entirely upon allo-antisera. Here we show how these alleles can be distinguished by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. The proposed PCR-RFLP approach allows the monitoring of IGHG locus diversity in rabbit.
Centro de Estudos de Ciência Animal, ICETA-UP, Campus Agrário de Vairão, Portugal.
P J
PJ
Esteves
P C
PC
Alves
N
N
Ferrand
W
W
van der Loo
D000818:Animals; D001483:Base Sequence; D004247:DNA; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011817:Rabbits / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D007143:Immunoglobulin Heavy Chains; D004247:DNA