Trophic effects of nootropic peptide preparations cerebrolysin and semax on cultured rat pheochromocytoma.
Trophic characteristics of neuroprotectors cerebrolysin and semax were evaluated by their capacity to induce differentiation and improve survival of cultured rat pheochromocytoma (PC12) cells. Morphological signs of cell differentiation (enlargement and formation of processes) were seen 24 h after addition of cerebrolysin into culture medium. Cerebrolysin improved survival of PC12 cells in serum-free medium. In a concentration of 100 microg/ml cerebrolysin decreased the content of apoptotic cells from 32% (control) to 10%. Semax produced no trophic effect on PC12 cells. hence, the neuroprotective effect of cerebrolysin in vivo probably results from trophic activity, while the protective effects of semax are mediated by other mechanisms.
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow.
E R
ER
Safarova
S I
SI
Shram
I A
IA
Grivennikov
N F
NF
Myasoedov
D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D016895:Culture Media, Serum-Free; D018697:Nootropic Agents / Q000494:pharmacology*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000473:pathology*; D051381:Rats; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D016895:Culture Media, Serum-Free; D018697:Nootropic Agents; D010446:Peptide Fragments; C006952:cerebrolysin; C048487:ACTH (4-7), Pro-Gly-Pro-; D000324:Adrenocorticotropic Hormone