Analysis of 5' Upstream Regulatory Sequences of Lignin Peroxidase(LIP) Genes of Phanerochaete chrysosporium.
Phanerochaete chrysosporium yields exocellular isozymes of lignin peroxidases and Mn peroxidases which are able to degrade lignin. In order to investigate the regulatory mechanism of LIP genes at transcriptional level, 6 DNA fragments subcloned from 5' upstream sequences of LIP genes (GLG3 and GLG6) were obtained, then gel mobility shift assay was carried out to screen DNA fragment(s) with ability to be bound specifically by proteins isolated from P.chrysosporium mycelia. The results showed that a 670 bp DNA fragment localized upstream of a LIP gene GLG6 was capable of being bound specifically by protein component, and analysis of its nucleotide sequence indicated that the DNA fragment contains the putative sequence characteristic of protein binding site. This result suggests that protein(s) in this fungus interact with the putative cis-element(s) in the 5' upstream sequence of LIP genes, regulating the expression of LIP genes.
Molecular Biology Laboratory, College of Life Science, Sichuan University, Chengdu 610064, China. nic3602@scu.edu.cn
Hong
H
Feng
Yi-Zheng
YZ
Zhang