Diversity of rhizobia from nodules of the leguminous tree Gliricidia sepium, a natural host of Rhizobium tropici.
The Rhizobium species that nodulate the legume tree Gliricidia sepium were analyzed by phenotypic characteristics (including nodule formation in different hosts), PCR-RFLP patterns and sequences of 16S rRNA genes, multilocus enzyme electrophoresis, and plasmid patterns. Strains of Rhizobium tropici type A and B, Sinorhizobium spp., and Rhizobium etli bv. phaseoli were encountered in G. sepium nodules and their presence depended on the site sampled.
Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Morelos.
Carlos
C
Acosta-Durán
Esperanza
E
Martínez-Romero
D007887:Fabaceae / Q000382:microbiology*; D005798:Genes, Bacterial; D014644:Genetic Variation; D008800:Mexico; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D014197:Trees / Q000382:microbiology*
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S