Development of hydrogel implants for urinary incontinence treatment.
Hydrogel implants for urinary incontinence treatment based on HEMA supplemented with 10% methacrylic acid have been developed. The swelling properties of implants were tested in vitro and in vivo after implantation to laboratory mice. Biocompatibility has been determined by incubation of implants in tissue culture, by histological examination of adjacent tissues after subcutaneous application of implants to laboratory mouse in a long-term experiment, and by flow cytometry examination of blood cells. The swelling of hydrogel implants was completed in 6-24 h. There was no effect on in vitro growth of cells incubated with implants. In mice, implants were well tolerated without any sign of inflammatory reaction. The material allows an elastic compression of urethra compensating a damaged sphincter after trans-urethral sub-mucosal implantation of hydrogel cylinders.
Institute of Pathophysiology, First Faculty of Medicine, Charles University, Prague, Czech Republic. sefc@cesnet.cz
L
L
Sefc
M
M
Prádný
J
J
Vacík
J
J
Michálek
C
C
Povýsil
I
I
Vítková
M
M
Halaska
V
V
Simon
D000818:Animals; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D006410:Hematopoiesis; D020100:Hydrogels; D066298:In Vitro Techniques; D008689:Methacrylates; D051379:Mice; D008807:Mice, Inbred BALB C; D019736:Prostheses and Implants; D014549:Urinary Incontinence / Q000473:pathology / Q000601:surgery*
D020100:Hydrogels; D008689:Methacrylates; C005044:hydroxyethyl methacrylate