Stromal cell-derived factor (SDF) 1-3'A polymorphism and sequences in Thais.
Stromal cell derived factor (SDF) 1-3'A polymorphism in Thai subjects was determined by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products using a restriction enzyme Msp 1 cleavage. The allelic frequency of SDF1-3'A in this population was 0.289. SDF1-3'A genotyping showed 9.52% SDF1-3'A/3'A, 38.89% SDF1-wt/3'A, and 51.59% SDF1-wt/wt. Two clones of Thai-SDF genes, BD41 and BD42, were isolated and sequenced. Wild type Thai-SDF1 (BD42), a 278 bp sequence was identically aligned to human pre B cell stimulating factor homoloque (SDF1-b) (GenBank accession number L-36033) from nucleotides 788 to 1,065. Homologous Thai SDF1-3'A (BD41), a 277 bp sequence differed by single nucleotide with adenine substitution to guanine at position 880 of the SDF1-b, is the first evidence of SDF1-3'A polymorphism in Thais.
Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Pornthep
P
Tiensiwakul
D020413:3' Untranslated Regions / Q000235:genetics*; D000328:Adult; D001483:Base Sequence; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000235:genetics*; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D011446:Prospective Studies; D012689:Sequence Homology, Nucleic Acid; D013785:Thailand
D020413:3' Untranslated Regions; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC