The retinal pigment epithelium of Cr-deficient rats.
The purpose of this study is to investigate the effect of Cr deficiency on the rat retina. Three-week-old Wistar Kyoto rats were divided into 2 groups. Cr-deficient rats were fed AIN-93G diet without Cr and deionized distilled water. Control rats were fed AIN-93G diet and deionized distilled water. The Cr and sugar concentrations in the whole blood and cholesterol concentration in the serum were measured. We observed the retina with an electron microscope, and counted phagocytized lamellar structures in the retinal pigment epithelium (RPE) before and after the start of light exposure on negative electron microscopic films. The whole blood Cr level of Cr-deficient rats was less than 0.2 microg/l. The blood sugar level of Cr-deficient rats was significantly higher than that of normal rats (p < 0.05). There were significantly more phagocytized lamellar structures in the RPE of Cr-deficient rats 1, 2, 7, 11 and 12 h after the start of light exposure than in that of normal rats (p < 0.05). However, no morphological abnormalities were found in the photoreceptor cells of Cr-deficient rats. Phagocytosis in the photoreceptor outer segment discs in the RPE was accelerated, but the pattern of the retinal circadian rhythm with maximum phagocytosis 2 h after exposure to light was unchanged. The Cr-deficient state may cause the membrane to degenerate, and phagocytosis of the photoreceptor outer segment discs in the RPE may be accelerated. This study provided an evidence of the nutritional importance of Cr in rat retina.
Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
Yoshiko
Y
Ueda
Suketaka
S
Kanazawa
Huaqing
H
Gong
Noritake
N
Miyamura
Takashi
T
Kitaoka
Tsugio
T
Amemiya
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D002857:Chromium / Q000097:blood / Q000172:deficiency*; D002940:Circadian Rhythm; D008029:Lighting; D008297:Male; D010587:Phagocytosis / Q000502:physiology / Q000528:radiation effects; D017440:Photoperiod; D010786:Photoreceptor Cells / Q000502:physiology / Q000528:radiation effects / Q000648:ultrastructure; D010857:Pigment Epithelium of Eye / Q000502:physiology / Q000648:ultrastructure*; D051381:Rats; D011921:Rats, Inbred WKY; D012160:Retina / Q000473:pathology* / Q000502:physiology
D001786:Blood Glucose; D002857:Chromium; D002784:Cholesterol