[The association between HLA typing and different subtypes of Guillain Barré syndrome].
OBJECTIVE
To study the relationship between the susceptibility to two subtypes of Guillain-Barre syndrome(GBS) AIDP and AMAN and the frequency of HLA-class I and II alleles as well as to approach the characteristics of AIDP and AMAN patients in immune genetics.


METHODS
A case control research was done on 31 AIDP and 33 AMAN and 132 health individuals. DNA was extracted from peripheral blood leucocytes by improved fast salting out. HLA genes were typed with DNA-based technology and PCR-sequence specific primers (PCR-SSP) method.


RESULTS
HLA-A33 frequency of HLA-class I, and DR16 and DQ5 frequencies of HLA-class II showed a significant increase in AIDP group as compared with the control. Relative risk (RR) was 6.13, 8.28 and 3.47 respectively. Corrected probability (Pc) was 0.011, 0.014 and 0.025(P < 0.05). HLA-B15,B35 frequency of HLA-class I showed a significant increase in patients with AMAN as compared with the controls,RR was 4.09 and 7.08 respectively, Pc was 0.015 and 0.000 8 respectively.


CONCLUSIONS
HLA-A33, DR15 and DQ5 may have association with susceptibility to AIDP; HLA-B15, B35 may have association with susceptibility to AMAN. HLA-class II genes were not found to have any association with AMAN.
Department of Neurology, The Second Affilliated Hospital, Hebei Medical University, Shijiazhuang 050000, China.
Li
L
Guo
Weiping
W
Wang
Chunyan
C
Li
Ruichun
R
Liu
Guirong
G
Wang
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020275:Guillain-Barre Syndrome / Q000235:genetics* / Q000276:immunology; D015234:HLA-A Antigens / Q000145:classification / Q000235:genetics*; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics*; D006682:HLA-DP Antigens / Q000145:classification / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000145:classification / Q000235:genetics*; D006684:HLA-DR Antigens / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D019846:Miller Fisher Syndrome / Q000235:genetics* / Q000276:immunology
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens