Convenient synthesis of human calcitonin and its methionine sulfoxide derivative.
The human calcitonin peptide chain was assembled using Fmoc solid-phase peptide synthesis chemistry. The combinations of cleavage Reagent H with trans-[Pt(en)(2)Cl(2)](2+) and Reagents B, K, and R with trans-[Pt(CN)(4)Cl(2)](2-) provide convenient methods for the synthesis of human calcitonin and its methionine sulfoxide derivative; the formation of intramolecular disulfide bonds by the above Pt(IV) oxidants is essentially quantitative.
Shi
Tiesheng
T
Department of Chemistry, University of California, Riverside, CA 92521, USA.
Rabenstein
Dallas L
DL
D000595:Amino Acid Sequence; D002116:Calcitonin / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008715:Methionine / Q000031:analogs & derivatives* / Q000737:chemistry*; D008969:Molecular Sequence Data
D004220:Disulfides; D002116:Calcitonin; D008715:Methionine; C013111:methionine sulfoxide