Identification of the peptide motifs that interact with HLA-DR8 (DRB1*0802) in Streptococcus mutans proteins.
A glucosyltransferase (GTF) and a surface protein antigen (PAc) of Streptococcus mutans have been suggested as possible components of an effective dental caries vaccine. To identify antigenic peptides in GTF and PAc that bind to MHC class II (HLA-DR8, DRB1*0802) molecules, we investigated binding activities to DR8 molecules of overlapping synthetic peptides at several sites in GTF and in the alanine-rich repeating region of PAc using an ELISA-inhibition competitive binding assay for the interaction between the HLA-DR molecule and the PAc (316-334) peptide. Six GTF peptides and 10 PAc peptides strongly bound to the HLA-DR8 molecule. In a homology analysis of the amino acid sequences of the six GTF peptides, two binding motifs were found in L/Y--Y/L-A/N and Y/L--N/G/E--Y-V/L/P. Moreover, a new binding motif in PAc was found in L--Y-A. It is suggested that these binding motifs could be useful in designing a dental caries vaccine in humans.
Nomura
Y
Y
Department of Oral Science, National Institute of Infectious Diseases, Tokyo, Japan.
Eto
A
A
Hanada
N
N
Senpuku
H
H
D020816:Amino Acid Motifs; D001422:Bacterial Adhesion / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry*; D001428:Bacterial Vaccines; D001667:Binding, Competitive; D003731:Dental Caries / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay; D005964:Glucosyltransferases / Q000737:chemistry*; D006684:HLA-DR Antigens / Q000378:metabolism*; D059810:HLA-DR Serological Subtypes; D006801:Humans; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis; D013295:Streptococcus mutans / Q000201:enzymology / Q000276:immunology*
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C041559:HLA-DR8 antigen; D008562:Membrane Glycoproteins; D010455:Peptides; C055764:S-layer proteins; D005964:Glucosyltransferases