Oxytocin stimulates proliferation of human osteoblast-like cells.
Oxytocin receptors have recently been demonstrated in human osteoblast-like (hOB) cells. In this study, oxytocin 100-1000 pmol/l increased cell proliferation of primary cultures of hOB cells, measured by [3H]thymidine incorporation, (P<0.01). In human osteosarcoma cell-line (SaOS-2), oxytocin 100 pmol/l increased cell proliferation (measured by [3H]thymidine incorporation and a commercially available kit) and protein synthesis ([3H]proline incorporation) (P<0.05). The increase in cell proliferation was abolished when SaOS-2 cells were incubated with an oxytocin antagonist and oxytocin. Oxytocin 100 pmol/l decreased interleukin-6 (IL-6) production of the hOB cells (23.4+/-1.96 versus 33.4+/-2.65 pg/well; P<0.001). These findings indicate that oxytocin may affect bone metabolism in humans.
Department of Molecular Medicine, Endocrine and Diabetes Unit, Karolinska Institutet and Karolinska Hospital, S-171 76, Stockholm, Sweden. maria.petersson@fyfa.ki.se
Maria
M
Petersson
Alena
A
Lagumdzija
André
A
Stark
Elisabet
E
Bucht
D001842:Bone and Bones / Q000378:metabolism; D002455:Cell Division; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D053244:Osteoprotegerin; D010121:Oxytocin / Q000494:pharmacology*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D018045:Receptors, Oxytocin / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism; D014407:Tumor Cells, Cultured
D006023:Glycoproteins; D015850:Interleukin-6; D053244:Osteoprotegerin; D018160:Receptors, Cytoplasmic and Nuclear; D018045:Receptors, Oxytocin; D018124:Receptors, Tumor Necrosis Factor; C506030:TNFRSF11B protein, human; D010121:Oxytocin; D016173:Macrophage Colony-Stimulating Factor; D000242:Cyclic AMP