Energy metabolism during microsurgical transfer of human skeletal muscle assessed by high-pressure liquid chromatography and by 31P-nuclear magnetic resonance.
The effect of ischaemia and reperfusion on human skeletal muscle was studied during free vascularised muscle transfer. Muscle biopsy specimens were taken from patients having microsurgical muscle transfer, 18 cases (17 patients; 12 men, 5 women). The biopsies were taken three times: before transfer of the muscle (control), at maximum ischaemic time, and one hour after revascularisation. The biopsy specimens were analysed for purine nucleotides, by high-pressure liquid chromatography (HPLC), and by nuclear magnetic resonance (NMR) at 500 MHz. Phosphocreatine (PCr) recovered only partially (79%) and adenosine triphosphate (ATP) did not differ significantly from normal control after revascularisation and a mean ischaemic time of 114 minutes. NMR measurements showed an accumulation of glucose-6-phosphate (G-6-P) during the ischaemic period, indicating anaerobic metabolism. After three hours of ischaemia and one hour of reperfusion the PCr recovery was less than 60% (r = 0.7). The results confirm those of previous animal studies, which set three hours normothermic ischaemia as a safe limit for tissue preservation when transferring skeletal muscle. Longer ischaemic times may cause serious postoperative healing problems and reduced muscle function.
Department of Plastic Surgery, Sahlgrenska University Hospital, Göteborg University, Göteborg, Sweden.
Jonas
J
Lundberg
Anna
A
Elander
Olivier
O
Rakotonirainy
Therese
T
Zetterlund
Ingemar
I
Fogdestam
Bassam
B
Soussi
D000255:Adenosine Triphosphate / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D002851:Chromatography, High Pressure Liquid; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D019298:Glucose-6-Phosphate / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism* / Q000637:transplantation*; D010725:Phosphocreatine / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism*; D013524:Surgical Flaps / Q000502:physiology*; D013997:Time Factors; D014021:Tissue Preservation / Q000592:standards
D010725:Phosphocreatine; D019298:Glucose-6-Phosphate; D000255:Adenosine Triphosphate