Na+/H+ exchange in primary, secondary and n-butyrate-treated cultures of ruminal epithelial cells: short communication.
Rate of amiloride-sensitive Na+ uptake into cultured rumen epithelial cells was studied in order to clarify the influence of culture conditions on Na+/H+ exchange (NHE). Cell cultures were exposed to Na-n-butyrate or not for seven days or subcultured. On the 14th day of culturing, primary cell cultures without butyrate exposure showed both non-stratified and stratified growth. Na-n-butyrate treated 14-day-old cultures and 3-day-old subcultures contained mostly non-stratified, i.e. non-keratinised cells. Both n-butyrate treatment and subculturing increased total and amiloride-sensitive Na+ uptake. Our results indicate that Na+ uptake via NHE is determined by the amount and the ratio of non-stratified (non-keratinised) cells.
Gálfi
P
P
Department of Physiology and Biochemistry, Faculty of Veterinary Science, Szent István University, H-1400 Budapest, P.O. Box 2, Hungary. pgalfi@mail.univet.hu
Neogrády
Susan
S
Gäbel
G
G
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004232:Diuretics / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D012417:Rumen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*
D002087:Butyrates; D003470:Culture Media; D004232:Diuretics; D017923:Sodium-Hydrogen Exchangers; D000584:Amiloride