A screening system for enantioselective enzymes based on differential cell growth.
The esterase-catalyzed enantioselective hydrolysis of the fluoroacetate of pantolactone leads to fluoroacetic acid, a toxic compound which inhibits the growth of esterase-producing yeast; this forms the basis of an ee-assay.
Reetz
Manfred T
MT
Max-Planck-Institut für Kohlenforschung, D-45470 Mülheim/Ruhr, Germany. reetz@mpi-muelheim.mpg.de
Rüggeberg
Carsten J
CJ
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002455:Cell Division / Q000502:physiology*; D004950:Esterases / Q000378:metabolism*; D005463:Fluoroacetates / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006131:Growth Inhibitors / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000378:metabolism; D013237:Stereoisomerism
D005463:Fluoroacetates; D006131:Growth Inhibitors; C005412:pantolactone; C014815:fluoroacetic acid; D004950:Esterases; D015107:4-Butyrolactone