Phospholipase A2 activity in rat embryonic brain and in primary cultures of cortical neurons.
We investigated, with the aid of a radio-enzymatic method, the developmental changes in the activity of 85 kDa calcium-dependent cytosolic phospholipase A(2) (cPLA(2)) in pre-term embryonic rat brain and in primary cultures of cortical neurons. PLA(2) activity was highest in the brains of 18 day embryos (E18) and gradually decreased toward birth (E18 through E21). No significant differences were found regarding cPLA(2) activity in different topographies, namely hippocampus, cortex, and total brain. In primary cultures of E18/E19 cortical neurons, cPLA(2) activity was higher than the respective embryonic tissue activity, straight from early development. In vitro cPLA(2) activity peaked on the 4th day in culture, and decreased after the six days of incubation, when the cultures became mature. This result reinforces previous evidence that cPLA(2) plays an important role in the early development of the nervous system, and further suggests that it may be implicated in neurodevelopmental processes and in in vitro neuronal survival. We believe that we have produced a useful model for the tissue culture study of PLA(2) metabolism and biochemistry, which can be further addressed to the investigation of the biology of neurodegenerative and neuropsychiatric disorders.
Laboratory of Neuroscience (LIM-27), Department and Institute of Psychiatry, Faculty of Medicine, University of São Paulo, Brazil. forlenza@usp.br
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Forlenza
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EL
Schaeffer
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WF
Gattaz
D000818:Animals; D001921:Brain / Q000196:embryology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology / Q000201:enzymology; D005314:Embryonic and Fetal Development; D009474:Neurons / Q000201:enzymology*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D010741:Phospholipases A; D054467:Phospholipases A2