Transcriptome profiling to identify genes involved in peroxisome assembly and function.
Yeast cells were induced to proliferate peroxisomes, and microarray transcriptional profiling was used to identify PEX genes encoding peroxins involved in peroxisome assembly and genes involved in peroxisome function. Clustering algorithms identified 224 genes with expression profiles similar to those of genes encoding peroxisomal proteins and genes involved in peroxisome biogenesis. Several previously uncharacterized genes were identified, two of which, YPL112c and YOR084w, encode proteins of the peroxisomal membrane and matrix, respectively. Ypl112p, renamed Pex25p, is a novel peroxin required for the regulation of peroxisome size and maintenance. These studies demonstrate the utility of comparative gene profiling as an alternative to functional assays to identify genes with roles in peroxisome biogenesis.
The Institute for Systems Biology, 1441 N. 34th Street, Seattle, WA 98103-8904, USA.
Jennifer J
JJ
Smith
Marcello
M
Marelli
Rowan H
RH
Christmas
Franco J
FJ
Vizeacoumar
David J
DJ
Dilworth
Trey
T
Ideker
Timothy
T
Galitski
Krassen
K
Dimitrov
Richard A
RA
Rachubinski
John D
JD
Aitchison
D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D020675:Peroxisomes / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D015533:Transcriptional Activation