Energy transfer method in membrane studies: some theoretical and practical aspects.
Some applications of resonance energy transfer (RET) method to distance estimation in membrane systems are considered. The model of energy transfer between donors and acceptors randomly distributed over parallel planes localized at the outer and inner membrane leaflets is presented. It is demonstrated that RET method can provide evidence for specific orientation of the fluorophore relative to the lipid-water interface. An approach to estimating the depth of the protein penetration in lipid bilayer is suggested.
Department of Physics and Technology, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov 61077, Ukraine.
Galina P
GP
Gorbenko
Yegor A
YA
Domanov
D001703:Biophysics / Q000379:methods*; D004735:Energy Transfer; D005456:Fluorescent Dyes; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D008566:Membranes; D015233:Models, Statistical; D014867:Water
D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008055:Lipids; D014867:Water