Characterization of a stamen-specific cDNA encoding a novel plant defensin in Chinese cabbage.
We isolated a stamen-specific cDNA, BSD1 (Brassica stamen specific plant defensin 1) that encodes a novel plant defensin peptide in Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant defensins are antimicrobial peptides containing eight highly conserved cysteine residues linked by disulfide bridges. In BSD1, the eight cysteine residues and a glutamate residue at position 29 are conserved whereas other amino acid residues of the plant defensins consensus sequence are substituted. BSD1 transcripts accumulate specifically in the stamen of developing flowers and its level drops as the flowers mature. The recombinant BSD1 produced in Escherichia coli showed antifungal activity against several phytopathogenic fungi. Furthermore, constitutive over-expression of the BSD1 gene under the control of the cauliflower mosaic virus (CaMV) 35S promoter conferred enhanced tolerance against the Phytophthora parasitica in the transgenic tobacco plants.
Park
Hyeong Cheol
HC
Division of Applied Life Science, Gyeongsang National University, Chinju, Korea.
Kang
Yun Hwan
YH
Chun
Hyun Jin
HJ
Koo
Ja Choon
JC
Cheong
Yong Hwa
YH
Kim
Cha Young
CY
Kim
Min Chul
MC
Chung
Woo Sik
WS
Kim
Jong Cheol
JC
Yoo
Jae Hyuk
JH
Koo
Yoon Duck
YD
Koo
Sung Chul
SC
Lim
Chae Oh
CO
Lee
Sang Yeol
SY
Cho
Moo Je
MJ
D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology; D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D023082:Defensins; D004305:Dose-Response Relationship, Drug; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000494:pharmacology; D018514:Plant Structures / Q000235:genetics*; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000494:pharmacology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology
D000935:Antifungal Agents; D018076:DNA, Complementary; D023082:Defensins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins