The biological effects of antiadhesion agents on activated RAW264.7 macrophages.
The objective of this study is to determine the biological effects of various antiadhesion agents on macrophages, which play an essential role in wound healing and adhesion. To determine these effects, RAW264.7 macrophages were activated with lipopolysaccharide in the presence of antiadhesion agents: oxidized regenerated cellulose (oxyC), sodium hyaluronate (HA), dexamethasone (Dex), or chondroitin sulfate (CS). The release of nitric oxide (NO), vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), or matrix metalloproteinases (MMPs) from RAW264.7 was measured. We found that oxyC reduced the release of NO, IL-6, MMP-2, and MMP-9, whereas it enhanced the release of VEGF. HA reduced the release of MMP-2, whereas it enhanced the release of VEGF and NO. HA exhibited no significant effect on the release of IL-6 or MMP-9. Dex reduced the release of NO, VEGF, IL-6, MMP-2, and MMP-9. CS reduced the release of VEGF, IL-6, and MMP-2, although it had no significant effect on the release of NO and MMP-9. Antiadhesion agents, which have been clinically used as physical barriers, modulated the functions of macrophages.
Department of Obstetrics and Gynecology, Okayama University Medical School, 2-5-1 Shikata, Okayama-City, Okayama, 700-8558 Japan.
Toshihiro
T
Habara
Mikiya
M
Nakatsuka
Hideki
H
Konishi
Kazuo
K
Asagiri
Soichi
S
Noguchi
Takafumi
T
Kudo
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001672:Biocompatible Materials; D002460:Cell Line; D002483:Cellulose, Oxidized / Q000494:pharmacology*; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D016228:Endothelial Growth Factors / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008222:Lymphokines / Q000378:metabolism; D008262:Macrophage Activation; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010537:Peritoneum; D000267:Tissue Adhesions / Q000188:drug therapy*; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014945:Wound Healing / Q000502:physiology
D000893:Anti-Inflammatory Agents; D001672:Biocompatible Materials; D002483:Cellulose, Oxidized; D016228:Endothelial Growth Factors; C060002:INTERCEED; D036341:Intercellular Signaling Peptides and Proteins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D008222:Lymphokines; D009573:Nitrites; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D009569:Nitric Oxide; D003907:Dexamethasone; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9