Cancer prevention and therapy with kiwifruit in Chinese folklore medicine: a study of kiwifruit extracts.
Kiwi gold fruits were extracted successively with hexane, acetone, methanol and 70% methanol, and further fractionated by silica gel and ODS column chromatographies for the assays of various biological activities. Five fractions H1, H2 (hexane extract), Al, A2 (acetone extract) and M2 (methanol extract) showed selective cytotoxic activity against human oral tumor cell lines, which was more sensitive than human gingival fibroblasts. More hydrophilic fractions [70M3, 70M4, 70M5] of 70% methanol extract displayed higher anti-HIV activity, radical generation and O2- scavenging activity. The antibacterial activity of 70% methanol extracts [70M0, 70M1, 70M2, 70M3, 70M4] was generally lower than that of more lipophilic fractions (hexane, acetone, methanol extracts), although each fraction did not show any specific antimicrobial action. All fractions were inactive against Helicobacter pylori. These results demonstrate that gold kiwifruit extracts contain valuable, various bioactive materials, which can be separated with each other.
Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose-shi, Tokyo 204-8588, Japan. motohasi@my-pharm.ac.jp
Noboru
N
Motohashi
Yoshiaki
Y
Shirataki
Masami
M
Kawase
Satoru
S
Tani
Hiroshi
H
Sakagami
Kazue
K
Satoh
Teruo
T
Kurihara
Hideki
H
Nakashima
Ilona
I
Mucsi
Andreas
A
Varga
Joseph
J
Molnár
D029042:Actinidia / Q000737:chemistry*; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D041221:Candida glabrata / Q000187:drug effects; D002460:Cell Line; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000187:drug effects; D005347:Fibroblasts; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D005638:Fruit / Q000737:chemistry*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects; D006678:HIV / Q000187:drug effects / Q000502:physiology; D016480:Helicobacter pylori / Q000187:drug effects; D006801:Humans; D008516:Medicine, Chinese Traditional; D008826:Microbial Sensitivity Tests; D009062:Mouth Neoplasms / Q000473:pathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D014407:Tumor Cells, Cultured
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D016166:Free Radical Scavengers; D005609:Free Radicals; D010936:Plant Extracts