Base pairing properties of 8-oxo-7,8-dihydroadenosine in cDNA synthesis by reverse transcriptases.
Incorporation of nucleotides opposite 8-oxo-7,8-dihydroadenosine (8-oxoA) in oligonucleotides with dNTPs by three reverse transcriptases (AMV-, MMRV-, RAV2-RT) in cDNA synthesis was studied. Guanine as well as thymine was incorporated preferentially by all reverse transcriptases. In the melting temperature experiment, 8-oxoA and 8-oxoA-Me formed base pairs with thymine and guanine with similar stabilities.
Kim
Sang Kook
SK
Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu 702-701, Republic of Korea.
Kim
Ji Young
JY
Baek
Ae Kyeong
AK
Moon
Byung Jo
BJ
D001345:Autoradiography; D020029:Base Pairing; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000138:chemical synthesis*; D003839:Deoxyadenosines / Q000737:chemistry*; D003854:Deoxyribonucleotides / Q000737:chemistry; D006151:Guanosine / Q000031:analogs & derivatives; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry*; D013696:Temperature; D013698:Templates, Genetic; D013816:Thermodynamics; D013941:Thymine / Q000031:analogs & derivatives
D017931:DNA Primers; D018076:DNA, Complementary; D003839:Deoxyadenosines; D003854:Deoxyribonucleotides; D009841:Oligonucleotides; D006151:Guanosine; C078251:2'-deoxy-7,8-dihydro-8-oxoadenosine; D012313:RNA; D012194:RNA-Directed DNA Polymerase; D013941:Thymine