Analysis of repeatability in spotted cDNA microarrays.
We report a strategy for analysis of data quality in cDNA microarrays based on the repeatability of repeatedly spotted clones. We describe how repeatability can be used to control data quality by developing adaptive filtering criteria for microarray data containing clones spotted in multiple spots. We have applied the method on five publicly available cDNA microarray data sets and one previously unpublished data set from our own laboratory. The results demonstrate the feasibility of the approach as a foundation for data filtering, and indicate a high degree of variation in data quality, both across the data sets and between arrays within data sets.
Department of Computer and Information Science, Norwegian University of Science and Technology, NO-7491 Trondheim, Norway. tkj@idi.ntnu.no
Tor-Kristian
TK
Jenssen
Mette
M
Langaas
Winston P
WP
Kuo
Birgitte
B
Smith-Sørensen
Ola
O
Myklebost
Eivind
E
Hovig
D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards*; D015203:Reproducibility of Results; D013223:Statistics as Topic