Rapid parallel mutation scanning of gene fragments using a microelectronic protein-DNA chip format.
We have developed a method for the de novo discovery of genetic variations, including single nucleotide polymorphisms and mutations, on microelectronic chip devices. The method combines the features of electronically controlled DNA hybridisation on open-format microarrays, with mutation detection by a fluorescence-labelled mismatch- binding protein. Electronic addressing of DNA strands to distinct test sites of the chip allows parallel analysis of several individuals, as demonstrated for mutations in different exons of the p53 gene. This microelectronic chip-based mutation discovery assay may substitute for time-consuming sequencing studies and will complement existing technologies in genomic research.
Nanogen Recognomics GmbH, Industriepark Hoechst, Building G830, 65926 Frankfurt am Main, Germany.
Heike A
HA
Behrensdorf
Marc
M
Pignot
Norbert
N
Windhab
Andreas
A
Kappel
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics; D001426:Bacterial Proteins; D002232:Carbocyanines / Q000737:chemistry; D002460:Cell Line; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods*; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D051719:MutS DNA Mismatch-Binding Protein; D009154:Mutation / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013388:Succinimides / Q000737:chemistry; D020349:Surface Plasmon Resonance; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D001426:Bacterial Proteins; D002232:Carbocyanines; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D005456:Fluorescent Dyes; D013388:Succinimides; D016159:Tumor Suppressor Protein p53; C085321:cyanine dye 5; D004247:DNA; D000251:Adenosine Triphosphatases; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli