Internalization and trafficking of fluorescent-labeled phospholipids in yeast.
Phospholipid reporter molecules, containing a fluorescent group attached to a short, acyl chain, spontaneously insert into the plasma membrane of yeast cells allowing retrograde trafficking to intracellular organelles as well as their metabolic fates to be monitored. This approach provides the framework for determining the dependence of particular phospholipid trafficking and metabolic steps on a wide range of genes known to be required for related membrane transport functions as well as for developing genetic screens to identify novel genes required for these processes. This review presents an overview of insights gained into phospholipid trafficking and metabolism using this approach.
Nichols
J Wylie
JW
Department of Physiology, Emory University School of Medicine, Atlanta, GA 30322, USA. wnichols@physio.emory.edu
D002462:Cell Membrane / Q000378:metabolism; D005456:Fluorescent Dyes / Q000494:pharmacology*; D008954:Models, Biological; D010743:Phospholipids / Q000378:metabolism*; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D005456:Fluorescent Dyes; D010743:Phospholipids