Purification of NAD glycohydrolase from Agkistrodon acutus venom.
NAD glycohydrolase (NADase) from Agkistrodon acutus venom was purified to electrophoretic homogeneity by a fast, reproducible 3-step procedure including Q Sepharose Fast Flow, Superdex 75, and Mono S column chromatography. This new procedure gave a 15.6-fold purification with a recovery yield of 7.9% and a specific activity of 12.8 units/mg.
Wu
Shuang Ding
SD
Laboratory of Internal Friction and Defects in Solids, Institute of Solid State Physics, Chinese Academy of Sciences, Hefei 230031, China. sdwu@mail.iisp.ac.cn
Liu
Yanli
Y
Xu
Xiaolong
X
Zhu
Zhengang
Z
D017836:Agkistrodon; D000818:Animals; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009244:NAD+ Nucleosidase / Q000737:chemistry / Q000302:isolation & purification*
D003435:Crotalid Venoms; D009244:NAD+ Nucleosidase