Surfactant apoprotein A modulates interleukin-8 and monocyte chemotactic peptide-1 production.
Previous studies have shown that surfactant apoprotein A (SP-A) and natural or synthetic surfactant can modulate the release of pro-inflammatory cytokines from alveolar mononuclear phagocytes. The aim of this study was to assess whether SP-A or Surfactant (Surf) from patients with pulmonary alveolar proteinosis (PAP) can affect the release of two chemokines (interleukin (IL)-8 and monocyte chemtactic peptide (MCP)-1) from human monocytes and rat lung type-II cells. In addition IL-8 and MCP-1 levels were assessed in the brochoalveolar lavage fluid (BALF) of seven patients with PAP and compared with those in a group of control subjects (n=5). SP-A, tested over a wide range of concentrations, significantly increased IL-8 and MCP-1 release from monocytes. SP-A retained its activity after collagenase digestion, but was not active after heat treatment. The release of IL-8 by monocytes was also stimulated by Surf. Finally, median BALF IL-8 and MCP-1 levels in PAP patients were significantly higher than in controls (9.50 and 9.51 pg x mL(-1) in controls versus 151.95 and 563.70 pg x mL(-1) in PAP, respectively) and significantly correlated with SP-A concentrations in BALF. Overall the results of this study support the view that the high content of alveolar surfactant apoprotein A may contribute to the upregulation of chemokine release in pulmonary alveolar proteinosis, thus contributing to airway inflammation.
Meloni
F
F
Dept of Haematological, Pneumological and Cardiovascular Sciences, University of of Pavia and Instituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Italy. federica-meloni@libero.it
Alberti
A
A
Bulgheroni
A
A
Lupi
A
A
Paschetto
E
E
Marone Bianco
A
A
Rodi
G
G
Fietta
A
A
Luisetti
M
M
Baritussio
A
A
D000328:Adult; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011649:Pulmonary Alveolar Proteinosis / Q000276:immunology / Q000378:metabolism*; D037662:Pulmonary Surfactant-Associated Protein A / Q000494:pharmacology*; D051381:Rats; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D018932:Chemokine CCL2; D016209:Interleukin-8; D037662:Pulmonary Surfactant-Associated Protein A