In vitro antiplasmodial activity of extracts of Alchornea cordifolia and identification of an active constituent: ellagic acid.
Extracts of leaves of Alchornea cordifolia were studied for their antiplasmodial activities. Chloroformic and ether extracts were found to be inactive while the ethanolic extract exhibited mild in vitro activity against Plasmodium falciparum. Fractionation of this extract led us to isolate ellagic acid as the active constituent of the extract with IC(50) in the range of 0.2-0.5 microM. Cytotoxicity of ethanolic fraction and ellagic acid was also estimated on human fibroblasts cells (IC(50) on Hela cells = 7.3 microM at 24 h for ellagic acid).
Laboratoire de Chimie Organique Pharmaceutique, E.A. 2414, 15 Avenue Charles Flahault, Faculté de Pharmacie, 34060 Montpellier Cedex 2, France.
J-T
JT
Banzouzi
R
R
Prado
H
H
Menan
A
A
Valentin
C
C
Roumestan
M
M
Mallie
Y
Y
Pelissier
Y
Y
Blache
D000818:Animals; D000962:Antimalarials / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D004610:Ellagic Acid / Q000009:adverse effects / Q000302:isolation & purification* / Q000494:pharmacology*; D019665:Euphorbiaceae / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D008517:Phytotherapy; D010936:Plant Extracts / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects
D000962:Antimalarials; D010936:Plant Extracts; D004610:Ellagic Acid