Temporal and spatial expression of biologically active human factor VIII in the milk of transgenic mice driven by mammary-specific bovine alpha-lactalbumin regulation sequences.
Hemophilia A is one of the major inherited bleeding disorders caused by a deficiency or abnormality in coagulation factor VIII (FVIII). Hemophiliacs have been treated with whole plasma or purified FVIII concentrates. The risk of transmitting blood-borne viruses and the cost of highly purified FVIII are the major factors that restrict prophylaxis in hemophilia therapy. One of the challenges created by the biotechnology revolution is the development of methods for the economical production of highly purified proteins in large scales. Recent developments indicate that manipulating milk composition using transgenesis has focused mainly on the mammary gland as a bioreactor to produce pharmaceuticals. In the present study, a hybrid gene containing bovine alpha-lactalbumin and human FVIII cDNA was constructed for microinjection into the pronuclei of newly fertilized mouse eggs. The alphaLA-hFVIII hybrid gene was confirmed to be successfully integrated and stably germ-line transmitted in 12 (seven females/five males) lines. Western-blot analysis of milk samples obtained from eight of the transgenic founders and F1 offspring indicated that the recombinant hFVIII was secreted into the milk of the transgenic mice. The concentrations of rFVIII ranged from 7.0 to 50.2 microg/ml, over 35-200-fold higher than that in normal human plasma. Up to 13.4 U/ml of rFVIII was detected in an assay in which rFVIII restored normal clotting activity to FVIII-deficient human plasma.
Chen
Chuan-Mu
CM
Department of Zoology, National Chung Hsing University, Taichung, Taiwan, ROC. chchen1@dragon.nchu.edu.tw
Wang
Chih-Hong
CH
Wu
Shinn-Chih
SC
Lin
Chih-Cheng
CC
Lin
Shwu-Hwa
SH
Cheng
Winston T K
WT
D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D005169:Factor VIII / Q000235:genetics* / Q000378:metabolism; D005260:Female; D018014:Gene Transfer Techniques; D005809:Genes, Regulator; D006801:Humans; D007768:Lactalbumin / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000378:metabolism; D008894:Milk Proteins / Q000096:biosynthesis / Q000235:genetics
D008894:Milk Proteins; D005169:Factor VIII; D007768:Lactalbumin