Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk.
Preparations of anti-DNA sIgA were obtained from human milk by sequential chromatography on protein A-sepharose, DEAE-fractogel and DNA-cellulose. The influence of oligonucleotides on protein kinase activity was investigated. It was discovered that incubation of anti-DNA sIgA with oligodeoxyriboadenylate d(A)12 stimulates the phosphorylation of polypeptides of sIgA in the presence of [gamma-32P]ATP. The greatest was the incorporation of P into the sIgA H-chains. We also demonstrated stimulation of the casein kinase activity of anti-DNA sIgA by d(A)12. The stimulation of the protein kinase activity of anti-DNA sIgA by oligoriboadenylate r(A)12 was not detected.
Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv. kit@biochem.viv.ua
Yuri
Y
Kit
Elena
E
Kuligina
Dimitry
D
Semenov
Vladimir
V
Richter
D002847:Chromatography, Agarose; D002848:Chromatography, DEAE-Cellulose; D004247:DNA / Q000276:immunology*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology* / Q000302:isolation & purification; D008895:Milk, Human / Q000276:immunology*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism
D007070:Immunoglobulin A; D009838:Oligodeoxyribonucleotides; C030570:oligodeoxyadenylic acid; D004247:DNA; D011494:Protein Kinases