Callus cultures of Annona squamosa for the production of annonaceous acetogenins.
Callus cultures of Annona squamosa were induced using different explants including petals, seed contents (megagametophyte and embryo) and fruits (mesocarp). Growth of the calli induced from the explants was found to be influenced by the type, concentration and ratio of auxin vs. cytokinin. The content of squamocin (67.8 microg g(-1) dry weight) in calli cultured on Gamborg B-5 medium containing 5.0 mg l(-1) naphthalene acetic acid and 4.0 mg l(-1) zeatin was nearly seven times higher than that in intact fruits.
School of Pharmaceutical Sciences, Peking University, Beijing, China.
Li-Da
LD
Wang
De-You
DY
Qiu
Ji-Yun
JY
Chen
Yi-Fan
YF
Han
Jun-Hua
JH
Zheng
De-An
DA
Guo
D031041:Annona; D046508:Culture Techniques; D003583:Cytokinins; D035264:Flowers / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D005663:Furans / Q000378:metabolism*; D006801:Humans; D007210:Indoleacetic Acids; D007783:Lactones / Q000378:metabolism*; D009280:Naphthaleneacetic Acids; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism; D012038:Regeneration / Q000502:physiology; D012639:Seeds / Q000737:chemistry; D015026:Zeatin
D003583:Cytokinins; D005663:Furans; D007210:Indoleacetic Acids; D007783:Lactones; D009280:Naphthaleneacetic Acids; D010936:Plant Extracts; C059660:squamocin; D015026:Zeatin