Cryptococcal encephalitis in thermally injured mice is accelerated by type 2 T-cell responses.
OBJECTIVE
To explore the pathogenic role of burn-associated type 2 T-cell responses on the development of cryptococcal encephalitis in mice with severe thermal injuries.


METHODS
Experimental Cryptococcus neoformans infection in normal mice was compared with that in thermally injured mice (TI mice), normal mice treated with a mixture of interleukin (IL)-4 and IL-10, or normal mice inoculated with burn-associated type 2 T cells.


METHODS
University research laboratory.


METHODS
Male BALB/c mice, 8 to 10 wks of age.


METHODS
We prepared four groups of mice as follows: a) normal mice, b) TI mice, c) normal mice treated with the IL-4/IL-10 mixture, and d) normal mice inoculated with burn-associated type 2 T cells. These groups of mice were anesthetized and exposed to 1 x 10 cells/mouse of C. neoformans intratracheally. Cryptococcal growth in brains and lungs in normal mice were compared with those of the other three groups. Also, cytokine-producing profiles of T lymphocytes from brains of both normal mice and TI mice were determined.


RESULTS
Compared with normal mice, TI mice were susceptible to C. neoformans infection. At the maximum (15 days after infection), numbers of C. neoformans organisms in brains of TI mice were 10 times higher than those of the pathogen in brains of normal mice. After stimulation with anti-CD3 monoclonal antibody, IL-4 (but not interferon gamma) was produced in cultures of T lymphocytes from brains of TI mice 15 days after the infection, whereas the same cell preparation from normal mice produced interferon gamma (but not IL-4). TI mice and mice that were treated with a IL-4/IL-10 mixture or inoculated with burn-associated type 2 T cells were equally susceptible to the cryptococcal infection.


CONCLUSIONS
Burn-associated type 2 T cells or their cytokine products play a key role in the severity of cryptococcal encephalitis that develops in TI mice.
Department of Internal Medicine, The University of Texas Medical Branch, Galveston, TX 77555-0435, USA.
Katsunori
K
Furukawa
Makiko
M
Kobayashi
Hidetaka
H
Sasaki
David N
DN
Herndon
Richard B
RB
Pollard
Fujio
F
Suzuki
D000818:Animals; D002056:Burns / Q000150:complications / Q000276:immunology*; D003453:Cryptococcosis / Q000150:complications; D018450:Disease Progression; D004660:Encephalitis / Q000150:complications / Q000382:microbiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors