The Arabidopsis mutant cev1 links cell wall signaling to jasmonate and ethylene responses.
Biotic and abiotic stresses stimulate the synthesis of jasmonates and ethylene, which, in turn, induce the expression of genes involved in stress response and enhance defense responses. The cev1 mutant has constitutive expression of stress response genes and has enhanced resistance to fungal pathogens. Here, we show that cev1 plants have increased production of jasmonate and ethylene and that its phenotype is suppressed by mutations that interrupt jasmonate and ethylene signaling. Genetic mapping, complementation analysis, and sequence analysis revealed that CEV1 is the cellulose synthase CeSA3. CEV1 was expressed predominantly in root tissues, and cev1 roots contained less cellulose than wild-type roots. Significantly, the cev1 mutant phenotype could be reproduced by treating wild-type plants with cellulose biosynthesis inhibitors, and the cellulose synthase mutant rsw1 also had constitutive expression of VSP. We propose that the cell wall can signal stress responses in plants.
Ellis
Christine
C
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom.
Karafyllidis
Ioannis
I
Wasternack
Claus
C
Turner
John G
JG
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism; D002473:Cell Wall / Q000187:drug effects / Q000235:genetics*; D002482:Cellulose / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D003624:Darkness; D005030:Ethylenes / Q000096:biosynthesis* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D005964:Glucosyltransferases / Q000235:genetics; D018546:Hypocotyl / Q000254:growth & development; D008027:Light; D009154:Mutation; D054883:Oxylipins; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D015183:Restriction Mapping; D015398:Signal Transduction / Q000235:genetics*; D013314:Stress, Mechanical
D029681:Arabidopsis Proteins; C112279:COI1 protein, Arabidopsis; D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant; C011006:jasmonic acid; D002482:Cellulose; C036216:ethylene; C110227:CESA1 protein, Arabidopsis; D005964:Glucosyltransferases