Plating densities, alpha-difluoromethylornithine effects and time dependence on the proliferation of IEC-6 cells.
OBJECTIVE
To characterize the role of plating densities and alpha-difluoromethylornithine (DFMO) on the proliferation of IEC-6 cells in vitro.


METHODS
IEC-6 cells were seeded in 96-well microplates at various densities in the presence or absence of DFMO. Cells were counted and their proliferative capability was monitored Days 1 to 7 with MTT assay at an optical density of 570 nm.


RESULTS
There was a positive relationship between cell number and OD value (r = 0.954, P < 0.01). Higher plating densities (> 0.5 x 10(4) cells/well) inhibited the growth of cells on Day 2. When the density reaches 4 x 10(4) cells/well, the OD value increased gradually and reached a peak on Day 5. After that, the OD value began to fall. The growth of IEC-6 cells was limited at a low density (0.2 x 10(4) cells/well) on Day 4. DFMO caused a complete inhibition of proliferation of IEC-6 cells on Days 1 to 3.


CONCLUSIONS
Proliferation of IEC-6 cells is related to plating density and incubation time. It is inhibited by DFMO, but is reversible when the incubation time is prolonged.
Piwei Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Zili
Z
Zhang
Weiwen
W
Chen
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D000518:Eflornithine / Q000494:pharmacology*; D013997:Time Factors
D000970:Antineoplastic Agents; D000518:Eflornithine