Molecular characterization of phenotypically CAMP-negative Streptococcus agalactiae isolated from bovine mastitis.
In the present study three phenotypically CAMP-negative Streptococcus agalactiae, isolated from three cows with mastitis, were characterized by molecular analysis. An identification of the S. agalactiae was performed by conventional methods and by PCR amplification of species specific parts of the 16S rRNA gene and the 16S-23S rDNA intergenic spacer region. In addition all three phenotypically CAMP-negative isolates harboured a normal sized CAMP-factor encoding cfb gene indicating a reduced expression of CAMP-factor or a gene defect elsewhere along the pathway of expression. The clonal identity of the three isolates could be demonstrated by macrorestriction analysis of their chromosomal DNA.
Institut für Tierärztliche Nahrungsmittelkunde, Justus-Liebig-Universität, Giessen, Germany.
A A
AA
Hassan
O
O
Akineden
C
C
Lämmler
R
R
Huber-Schlenstedt
D000818:Animals; D001426:Bacterial Proteins; D002417:Cattle; D021903:DNA, Ribosomal Spacer; D005260:Female; D006460:Hemolysin Proteins; D008414:Mastitis, Bovine / Q000382:microbiology*; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013292:Streptococcus agalactiae / Q000145:classification / Q000235:genetics*
D001426:Bacterial Proteins; C039092:CAMP protein, Streptococcus; D021903:DNA, Ribosomal Spacer; D006460:Hemolysin Proteins; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S