Liquid chromatographic resolution of racemic amines, amino alcohols and related compounds on a chiral crown ether stationary phase.
A chiral stationary phase (CSP) based on diphenyl-substituted 1,1'-binaphthyl crown ether was applied in resolving various racemic amines, amino alcohols and alpha-aminocarbonyl compounds including pharmaceutically important compounds such as amphetamine analogues, mexiletine, norepinephrine and norephedrine. The resolution was quite successful. In order to find out the effects of mobile phase additives on the chromatographic resolution behaviors, four selected racemic compounds were resolved on the CSP with the variation of the type and content of organic, acidic and cationic modifiers in aqueous mobile phase and with the variation of column temperature. The resolution behaviors were quite dependent on the type and the content of organic, acidic and cationic modifiers in aqueous mobile phase and on column temperature.
Hyun
Myung Ho
MH
Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, South Korea. mhhyun@pusan.ac.kr
Han
Sang Cheol
SC
Lipshutz
Bruce H
BH
Shin
Young-Jun
YJ
Welch
Christopher J
CJ
D000438:Alcohols / Q000737:chemistry / Q000302:isolation & purification*; D000588:Amines / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D004988:Ethers, Cyclic / Q000737:chemistry*; D013237:Stereoisomerism
D000438:Alcohols; D000588:Amines; D004988:Ethers, Cyclic