Red cell storage lesion assessed by the levels of potassium, haemoglobin and Annexin V in supernatants.
The conventional and a new marker of global cellular lesion (Annexin V) are used to assess the processing/storage-induced changes in four types of RBC and filtered blood at 4 degrees and 22 degrees C, stored for a period of 35 days, in multi-satellite packs. It appears that mechanical trauma and presence of leucocytes and residual platelets have potential to increase levels of all markers of storage lesion, but to a variable extend. We have also provided new evidence that multi-satellite packs can be safely used for up to 35 days for small volume transfusion to sick premature infants, in a well-managed system by administering several transfusions from the same donation, hence reducing donor exposure.
National Blood Service, London & SE, Colindale, UK. jseghatchian@hotmail.com
Jerard
J
Seghatchian
Pranee
P
Krailadsiri
D017304:Annexin A5 / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D001793:Blood Preservation / Q000379:methods / Q000592:standards*; D004912:Erythrocytes / Q000378:metabolism*; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D011188:Potassium / Q000032:analysis
D017304:Annexin A5; D015415:Biomarkers; D006454:Hemoglobins; D011188:Potassium