Simultaneous determination of zidovudine and nevirapine in human plasma by RP-LC.
A simple method for the simultaneous determination of zidovudine and nevirapine in human plasma by reversed-phase liquid chromatography with UV detection at 265 nm was developed. A solid-liquid extraction procedure with internal standard was applied to the samples prior to analysis. The system requires a Zorbax SB-C18 column, 250 x 4.6 mm I.D. and a mobile phase composed of potassium dihydrogen phosphate (10 mM; pH 6.5)-acetonitrile (83:17, v/v). Peak-areas are linear; correlation coefficients are better than 0.999; both inter- and intra-day accuracy and precision are lower than 15%. Extraction recoveries are higher than 90% for both zidovudine and nevirapine. The method proposed was employed to determine the levels of the two retroviral drugs in plasma from HIV infected human subjects.
Istituto Superiore di Sanità, Viale Regina Elena 299, Rome, Italy.
Emilia
E
Marchei
Luisa
L
Valvo
Roberta
R
Pacifici
Manuela
M
Pellegrini
Gianna
G
Tossini
Piergiorgio
P
Zuccaro
D019380:Anti-HIV Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D019829:Nevirapine / Q000097:blood*; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000097:blood*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D015215:Zidovudine / Q000097:blood*
D019380:Anti-HIV Agents; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine; D019829:Nevirapine