[Regulating role of various amino acids in development of apoptosis in organotypic culture of the nervous and lymphoid tissue].
The effect of aminoacids L-arginin, L-lysine, L-asparagin was investigated in organotypic tissue culture of brain cortex and spleen of 1-day old rats. The aminoacids in concentrations 0.05 and 0.1 ng/ml are active, inducing a less intensive growth zone as compared to the control, excluding the effect of asparagin on lymphoid tissue. Method of fluorescent staining shows a negative correlation between growth zone size and apoptotic cell number. The nerve and lymphoid tissue express apoptosis in response to aminoacids.
Chalisova
N I
NI
I. P. Pavlov Institute of Physiology, Russian Acad. Sci., St. Petersburg, 199034, Nab. Makarova, 6, Russia.
Pennijainen
V A
VA
Haase
G
G
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis; D001120:Arginine / Q000494:pharmacology; D001216:Asparagine / Q000494:pharmacology; D001921:Brain / Q000166:cytology / Q000187:drug effects*; D046508:Culture Techniques; D008239:Lysine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000187:drug effects*
D000596:Amino Acids; D001216:Asparagine; D001120:Arginine; D008239:Lysine