Effects of a mechanical barrier on the integration of cortical onlay bone grafts placed simultaneously with endosseous implant.
BACKGROUND
Previous experimental studies on onlay bone graft integration have shown either advantages or disadvantages to the use of mechanical barriers. This indicates that the role played by the biologic properties of transplanted bone and membrane in graft revascularization and bone remodeling has not yet been established. The outcomes regarding osseointegration of titanium dental implants applied in such a condition are still contradictory.


OBJECTIVE
The rabbit's radius model that is grafted onto the mandibular lower border and covered by membrane can reproduce a challenging experimental situation to preliminarily study the factors involved in osseointegration under deprived blood vessels source.


METHODS
Fourteen New Zealand White rabbits had a 2.5-cm segment of the right radius osteoectomized and fixed onto the right mandibular lower border using titanium screws. Two screw-shaped titanium implants (2.5 mm wide yen 2.5 mm long) were installed 7 mm apart in the mid length of the grafted bone. In experimental sites, the graft with the implants and graft-host bone junction were covered by expanded polytetrafluoroethylene (e-PTFE) membrane; control sites were left uncovered. Eight animals from the experimental group and six animals from the control group were sacrificed at 6 and 24 weeks after surgery. Ground sections obtained from en bloc tissues containing graft, implants, and recipient bone were subjected to histologic evaluation and histomorphometric analysis (area occupied by the graft and bone-to-implant contact).


RESULTS
The graft showed significantly more resorption after 24 weeks than at 6 weeks (p < or =.05) irrespective of the treatment (with or without membrane), although the amount of new bone was greater at 24 weeks in sites where a membrane was covering the graft. Compared with 6 weeks postoperatively, the bone-to-implant contact was considerably improved at 24 weeks (p < or =.05), and the membrane seemed beneficial for implant osseointegration when compared with unprotected sites (p .05). As a result of graft resorption, the amount of soft tissue was considerably expanded in sites beneath membrane, accompanied by a sustained process of trabecular bone deposition close to the barrier.


CONCLUSIONS
Cortical onlay grafts covered by membrane demonstrated delayed remodeling, probably as a consequence of a hindered process of graft revascularization. Grafts covered by membrane might rely on previous host bone resorption both to become revascularized and to remodel. The findings that the membrane-protected grafts were most resorbed at 24 weeks might be attributable to better implant osseointegration, because the fixtures were exposed to greater mechanical stimulation in these sites. key words: bone regeneration, implant osseointegration, onlay bone-graft
Department of Oral and Maxillofacial Surgery and Periodontics, Faculty of Dentistry of Ribeirao Preto, University of S o Paulo, Brazil.
Luiz Z
LZ
Salata
Lars
L
Rasmusson
Karl-Erik
KE
Kahnberg
D000818:Animals; D016723:Bone Remodeling / Q000502:physiology; D001862:Bone Resorption / Q000473:pathology; D001863:Bone Screws; D016025:Bone Transplantation / Q000473:pathology / Q000502:physiology*; D015921:Dental Implants; D005260:Female; D006085:Graft Survival; D008334:Mandible / Q000473:pathology / Q000601:surgery; D008567:Membranes, Artificial; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000502:physiology; D016348:Osseointegration; D010012:Osteogenesis / Q000502:physiology; D011138:Polytetrafluoroethylene; D011817:Rabbits; D018709:Statistics, Nonparametric; D013997:Time Factors; D014025:Titanium; D014945:Wound Healing
D015921:Dental Implants; D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene; D014025:Titanium