Comb-like copolymers as self-coating, low-viscosity and high-resolution matrices for DNA sequencing.
Comb-like copolymers with a polyacrylamide backbone and poly(N,N-dimethylacrylamide) grafts were prepared, as a way to combine the superior sieving properties of polyacrylamide with the self-coating properties of polydimethylacrylamide. These matrices function well in the absence of a capillary coating, and achieve separation performances for single-stranded DNA that are comparable to those of state-of-the-art long-chain linear polyacrylamide. Structural parameters such as the grafting density and the polymer molecular mass were varied, and good performance appears to be achieved with a relatively large range of parameters. Surprisingly, excellent separation is achieved even with matrices that have a viscosity as low as 200 mPa/s. A discussion of the physics underlying this behavior is provided.
Laboratoire de physico-chimie Curie, UMR CNRS 168, Institut Curie, Section de Recherche, Paris, France.
Valessa
V
Barbier
Brett A
BA
Buchholz
Annelise E
AE
Barron
Jean-Louis
JL
Viovy
D000178:Acrylamides; D000180:Acrylic Resins; D004247:DNA / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D011108:Polymers; D012212:Rheology; D017422:Sequence Analysis, DNA / Q000379:methods*; D014783:Viscosity; D000099166:Polyacrylamides
D000178:Acrylamides; D000180:Acrylic Resins; D004247:DNA; D011108:Polymers; D000099166:Polyacrylamides; C429790:poly(N,N-dimethylacrylamide)