[Interphase FISH analysis on histologic sections of fixed tissues for t(11;14) (q13;q32) detection in mantle cell lymphoma and t(8;14)(q24;q32) in Burkitt's lymphoma].
We describe an interphase FISH analysis for formol-fixed, paraffin-embedded tissue sections with commercial probes detecting the t(11;14)(q13;q32) in mantle cell lymphoma and the t(8;14)(q24;q32) in Burkitt's lymphoma. Staining of an adjacent section allowed identification of tumoral areas. The cut-off value was evaluated in reactive lymph nodes at 5% for both probes. An incomplete hybridization pattern was found in 18 to 26% of the nuclei but was always lower than the percentages of translocated cells in tumoral regions. A t(11;14) was detected in 4/4 mantle cell lymphomas and a t(8;14) in 2/3 Burkitt's lymphomas. Interphase FISH analysis of fixed-tissue sections is a reliable technique for the direct detection of lymphoma-associated translocations on routine histological material.
EA 2406 Histologie et Pathologie Moléculaire, Université de Bordeaux 2, 33076 Bordeaux, France. Rotureau@histo.ubordeaux2.fr
Marc-Antoine
MA
Belaud-Rotureau
Marie
M
Parrens
Pierre
P
Dubus
Michelle
M
Turmo
Gisèle
G
Lacroute
Laurence
L
Taine
Philippe
P
Vago
Antoine
A
De Mascarel
Jean Philippe
JP
Merlio
D002051:Burkitt Lymphoma / Q000235:genetics*; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D002898:Chromosomes, Human, Pair 8; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020522:Lymphoma, Mantle-Cell / Q000235:genetics*; D014178:Translocation, Genetic