Genotyping on a complementary metal oxide semiconductor silicon polymerase chain reaction chip with integrated DNA microarray.
A novel method for the fast identification of genetic material utilizing a micro-DNA amplification and analysis device (micro-DAAD) consisting of multiple PCR microreactors with integrated DNA microarrays was developed. The device was fabricated in Si-technology and used for the genotyping of Chinese medicinal plants on the basis of differences in the noncoding region of the 5S-rRNA gene. Successful amplification of the genetic material and the consecutive analysis of the fluorescent-labeled amplicons in the micro-DAAD by the integrated oligonucleotide probes were demonstrated. Parallel analysis was performed by loading the four PCR reactors of the micro-DAAD with different samples of 3-microL volume. Temperature sensors and heating elements of the micro-DAAD enable precise temperature control and fast cycling, allowing the rapid completion of a combined amplification and analysis (hybridization) experiment.
Trau
Dieter
D
Biotechnology Research Institute and Department of Biochemistry, Hong Kong University of Science and Technology, Hong Kong SAR, China.
Lee
Thomas M H
TM
Lao
Alex I K
AI
Lenigk
Ralf
R
Hsing
I-Ming
IM
Ip
Nancy Y
NY
Carles
Maria C
MC
Sucher
Nikolaus J
NJ
D005838:Genotype; D008838:Microcomputers; D018613:Microscopy, Confocal; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D015345:Oligonucleotide Probes; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence
D015345:Oligonucleotide Probes