Predicting the kinetics of cell spreading.
We apply the wetting theory to predict the kinetics of fibroblast spreading onto an adhesive substrate, under simplifying assumptions on the cell structure and geometry. Three main parameters are used: cytoplasmic viscosity, cortical tension, and cell-to-substrate adhesion energy. The viscosity and tension values are taken from previous micromechanical studies. The adhesion energy, ill known, is adjusted by fitting the model predictions to available experimental data of contact radius versus time. The agreement is quite good, justifying such a "macroscopic" view of cell morphology.
Frisch
Thomas
T
Université de Provence-St Jérome, IRPHE/Biophysique Case 252, Marseille, France.
Thoumine
Olivier
O
D000447:Aldehydes; D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement / Q000502:physiology*; D048429:Cell Size / Q000502:physiology; D002642:Chick Embryo; D002645:Chickens; D003198:Computer Simulation; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D008954:Models, Biological; D012212:Rheology; D012680:Sensitivity and Specificity; D013314:Stress, Mechanical; D013499:Surface Properties; D014783:Viscosity
D000447:Aldehydes; C007860:glutaconaldehyde