Bronchoalveolar lavage protein patterns in children with malignancies, immunosuppression, fever and pulmonary infiltrates.
Severe respiratory infections are a major cause of morbidity and mortality in children receiving immunosuppressive therapy for malignancies. The goal of this study was to assess the major changes in the protein patterns in these children. Bronchoalveolar lavage (BAL) fluids of seven control children and of ten children with malignancies and fever not responding to broad spectrum antibiotic treatment was separated by horizontal two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the isoelectric point range 3-9. We observed a large increase of alpha(1)-antitrypsin (p = 0.0004) and decreases of the immunoglobulin (Ig) binding factor, transthyretin and cystatin S. Significant changes occurred also in the small acidic proteins. The relative abundance of the IgG heavy and light chains may hinder the separation and identification of many minor protein spots located in the basic area of the gel, suggesting that their removal during sample preparation may be warranted. This study demonstrated significant alterations in BAL fluid proteome in immunosuppressed children with persistent fever and pulmonary infiltrates. Future target regions of interest were identified. Sample prefractionation and the selection of suitable narrow isoelectric point ranges will be necessary for optimized detection and separating conditions.
Neumann
Manfred
M
Kinderklinik and Kinderpoliklinik im Dr. von Haunerschen Kinderspital, Ludwig-Maximilians-University, Munich, Germany.
von Bredow
Christina
C
Ratjen
Felix
F
Griese
Matthias
M
D000293:Adolescent; D000740:Anemia / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid; D002648:Child; D002675:Child, Preschool; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D006801:Humans; D007165:Immunosuppression Therapy; D007938:Leukemia / Q000378:metabolism; D008171:Lung Diseases / Q000378:metabolism*; D008297:Male; D009369:Neoplasms / Q000378:metabolism*; D010865:Pilot Projects