Salicylate reduces susceptibility of Mycobacterium tuberculosis to multiple antituberculosis drugs.
Salicylate induces multiple antibiotic resistance in various bacterial species. Here we investigated the effect of salicylate on the susceptibility of Mycobacterium tuberculosis to a range of antituberculosis (anti-TB) drugs. In the presence of salicylate, the killing effects of isoniazid (INH), rifampin (RMP), ethambutol (EMB), streptomycin (STR), and p-aminosalicylate (PAS) were reduced, as shown with a tetrazolium redox dye viability assay and a bacterial survival assay. Salicylate-induced resistance was more pronounced for PAS, STR, and EMB but was not apparent for INH and RMP when salicylate and the anti-TB agents were incorporated into 7H11 plates. The significance of these findings for TB treatment needs to be further evaluated in vivo.
Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD 21205, USA.
Alain
A
Schaller
Zhonghe
Z
Sun
Yongping
Y
Yang
Akos
A
Somoskovi
Ying
Y
Zhang
D000995:Antitubercular Agents / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D009580:Nitroblue Tetrazolium; D012459:Salicylates / Q000494:pharmacology*
D000995:Antitubercular Agents; D012459:Salicylates; D009580:Nitroblue Tetrazolium