c-fos mRNA expression associated with PGF(2alpha)-induced nest-building behaviour in female pigs.
Domestic pigs, Sus scrofa, build a maternal nest on the day before parturition. A model for nest building has been established in pigs, in which exogenously administered prostaglandin (PG) F(2alpha) may be used to elicit nesting behaviour in cyclic, pseudopregnant and pregnant pigs. The central mechanisms mediating this response are unknown. The present study determined regional brain activity using semi-quantitative analysis of c-fos mRNA, after induction of nest-building behaviour by PGF(2alpha) in Large White pseudopregnant pigs. Oestradiol valerate injections (5 mg/day) were given on days 11-15 of the oestrous cycle to induce pseudopregnancy. The pigs were housed individually in pens (2.8 x 1.7 m) containing straw. On the test day (day 46 or 47 of pseudopregnancy) animals were injected with 3 ml saline (n=5) or 15 mg of PGF(2alpha) (Lutalyse, Upjohn; n=6) intramuscularly. Pigs treated with PGF(2alpha), but not saline, displayed bouts of rooting, pawing and gathering straw, which we interpret as nest building behaviour. The pigs were killed 65 min after treatment, which was 30 min after peak nest building activity, and the brain, uterus and ovaries removed for processing using in situ hybridisation. Saline-treated pigs had elevated levels of c-fos mRNA, compared to background, in the pituitary, corpus luteum and uterus, and a lower, but elevated, level of expression in cerebellum, cortex, hippocampus and olfactory bulb. PGF(2alpha)-treated pigs had significantly higher levels of c-fos mRNA expression than saline-treated pigs in the parvocellular and magnocellular regions of the hypothalamic paraventricular nucleus, the supraoptic nucleus (including the pars dorso-medialis), the neural lobe of the pituitary gland and the cerebellum. PGF(2alpha)-treated pigs also had significantly higher c-fos induction in corpus luteum. These data show that the pattern of c-fos mRNA expression in specific brain areas is different between pigs that show PGF(2alpha)-induced nest building and saline-injected controls.
Department of Neurobiology, The Babraham Institute, CB2 4AT, Cambridge, UK. tom.burne@bbsrc.ac.uk
T H J
TH
Burne
P J E
PJ
Murfitt
C L
CL
Gilbert
D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003338:Corpus Luteum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015237:Dinoprost / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D007030:Hypothalamo-Hypophyseal System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009425:Nesting Behavior / Q000187:drug effects / Q000502:physiology*; D011247:Pregnancy; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism*; D013552:Swine / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*; D014599:Uterus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D015237:Dinoprost