Golgi-associated filament networks in duct epithelial cells of rabbit submandibular glands: immunohistochemical light and electron microscopic studies.
The duct epithelial cells of rabbit submandibular glands expressed keratin 8, keratin 14, and actin in the supranuclear region, and these cytoskeletal proteins formed ring structures, approximately 1-2.5 microm in diameter. Ultrastructurally, these ring structures were observed as a 'Golgi-associated filament network' surrounding Golgi apparatuses. Double immunofluorescent studies showed that keratin 8 and keratin 14 formed keratin 8/14 filaments, and that these filaments and actin filaments colocalized as components of the Golgi-associated filament network. Our studies suggested that the Golgi-associated filament network maintains the complex structure and location of the Golgi apparatus of the duct epithelium of rabbit submandibular glands. Tubulin was distributed diffusely throughout the cytoplasm of columnar cells, but no special relationship was found between tubulin and the Golgi apparatus.
Department of Anatomy, Kawasaki Medical School, 577 Matsushima, Kurashiki 701-0192, Japan. chizuru@med.kawasaki-m.ac.jp
Chizuru
C
Ogawa
Hirohiko
H
Iwatsuki
Masumi
M
Suda
Kazunobu
K
Sasaki
D008841:Actin Cytoskeleton / Q000737:chemistry; D000199:Actins / Q000378:metabolism; D000818:Animals; D003599:Cytoskeleton / Q000737:chemistry*; D004847:Epithelial Cells / Q000648:ultrastructure*; D005260:Female; D006056:Golgi Apparatus / Q000648:ultrastructure*; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000737:chemistry; D053547:Keratin-14; D007633:Keratins / Q000378:metabolism; D008854:Microscopy, Electron; D008870:Microtubules / Q000737:chemistry; D011817:Rabbits; D013363:Submandibular Gland / Q000166:cytology*; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D053547:Keratin-14; D014404:Tubulin; D007633:Keratins