Discriminatory power of RAPD, PCR-RFLP and southern blot analyses of ureCD or ureA gene probes on Helicobacter pylori isolates.
The genetic diversity of 33 Nigerian Helicobacter pylori isolates were studied using RAPD, PCR-RFLP and Southern blot analysis of ureA or ureCD gene probes. RAPD was able to distinguish the following number of isolates using the primers 3880: 5'-AAGAGCCCGT-3' (28), 3881 :5'-AACGCGCAAC-3' (33) and OPH8 :5'-GAAACACCCC-3' (25). Southern blot analysis using the ureCD probe was also able to distinguish the 12 isolates tested into ten different patterns. The PCR-RFLP technique distinguished all 33 isolates into six types. In conclusion, considering typeability, discriminatory power, and convenience, RAPD with the 3881 primer was considered the most useful technique.
Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Yaba, Lagos. stellaismith@yahoo.com
Stella
S
Smith
Franck
F
Cantet
Fabrice
F
Angelini
Armelle
A
Marais
Francis
F
Mégraud
Ekkhehard
E
Bayerdöffer
Stephan
S
Miehlke
D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers; D005756:Gastritis / Q000382:microbiology; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D009549:Nigeria; D010733:Phosphoglucomutase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D012680:Sensitivity and Specificity; D014456:Ulcer / Q000382:microbiology; D014510:Urease / Q000235:genetics*
D001426:Bacterial Proteins; D017931:DNA Primers; C086767:UreD protein, Bacteria; D014510:Urease; C097631:phosphoglucosamine mutase; D010733:Phosphoglucomutase