Development of a flow-injection analysis (FIA) enzyme sensor for fructosyl amine monitoring.
An enzyme-sensor system with flow-injection analysis (FIA) has been developed for the detection of fructosyl amine compounds; the sensor utilizes fructosyl amine oxidase isolated from the marine yeast Pichia sp. N1-1 strain. With this FIA system 0.2 to 10 mmol L(-1) fructosyl valine can be determined. The sensor is approximately five times more sensitive to fructosyl valine, a model compound for glycated hemoglobin HbA1c, than to N(epsilon)-fructosyl lysine, a model compound for glycated albumin. This FIA system can also be used to detect fructosyl dipeptides. The operational stability of the sensor enabled more than 120 consecutive sample injections over a period of approximately 20 h.
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Japan.
Kinuko
K
Ogawa
Daniela
D
Stöllner
Frieder
F
Scheller
Axel
A
Warsinke
Fumimasa
F
Ishimura
Wakako
W
Tsugawa
Stefano
S
Ferri
Koji
K
Sode
D000594:Amino Acid Oxidoreductases; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D002138:Calibration; D004800:Enzymes, Immobilized; D006442:Glycated Hemoglobin / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis*; D014633:Valine / Q000031:analogs & derivatives* / Q000032:analysis*
D000594:Amino Acid Oxidoreductases; D004800:Enzymes, Immobilized; D006442:Glycated Hemoglobin; D006023:Glycoproteins; D008239:Lysine; D014633:Valine; C045420:fructosylvaline; C033186:fructosyl-lysine; C103012:amadoriase