Fluorometric determination of DNA using a new ruthenium complex Ru(bpy)2PIP(V) as a nucleic acid probe.
A new ligand, 2-phenyl(5-fluoro)imidazo[f]-1,10-phenanthroline (PIP(V)), and its coordination compounds, Ru(bpy)2PIP(V), were synthesized. The fluorescence spectrum of the interaction between Ru(bpy)2PIP(V) and DNA was studied, and a very strong fluorescence peak at a wavelength of 589 nm appeared. The optimum condition of analyzing DNA was decided. The method is simple, convenient and fast, and also has high sensitivity and good selectivity. It has been satisfactorily employed for determinations in synthesized samples.
Song
Gongwu
G
Analysis and Testing Center, Hubei University, Wuhan, China. songgw@hubu.edu.cn
Li
Ling
L
Liu
Liming
L
Fang
Guangrong
G
Lu
Shaofang
S
He
Zhike
Z
Zeng
Yun'e
Y
D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D005470:Fluorometry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D015341:Nucleic Acid Probes / Q000737:chemistry* / Q000378:metabolism*; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism*; D010618:Phenanthrolines / Q000737:chemistry* / Q000378:metabolism*; D012492:Salts / Q000494:pharmacology; D012680:Sensitivity and Specificity; D013053:Spectrophotometry
C465028:(bipyridine)(2-phenyl(5-fluoro)-imidazo(f)-1,10-phenathroline)ruthenium; C465029:2-phenyl(5-fluoro)-imidazo(f)-1,10-phenanthroline; D008024:Ligands; D015341:Nucleic Acid Probes; D009942:Organometallic Compounds; D010618:Phenanthrolines; D012492:Salts; D004247:DNA