Phase variation analysis of Coxiella burnetii during serial passage in cell culture by use of monoclonal antibodies.
Antigenic changes in Coxiella burnetii Nine Mile strain phase I during serial passages in cell culture were analyzed with three groups of monoclonal antibodies (MAbs) against lipopolysaccharide. The MAbs of group 1 did not react with organisms that were passaged over five times, and the MAbs of group 2 did not react with organisms that were passaged over eight times. The MAbs of group 3 reacted with organisms passaged up to 15 times but did not react with phase II cells. These results suggest that C. burnetii could be differentiated into four phase states during phase variation.
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
Akitoyo
A
Hotta
Midori
M
Kawamura
Ho
H
To
Masako
M
Andoh
Tsuyoshi
T
Yamaguchi
Hideto
H
Fukushi
Katsuya
K
Hirai
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000940:Antigenic Variation / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D016997:Coxiella burnetii / Q000254:growth & development / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides