Catalog of 605 single-nucleotide polymorphisms (SNPs) among 13 genes encoding human ATP-binding cassette transporters: ABCA4, ABCA7, ABCA8, ABCD1, ABCD3, ABCD4, ABCE1, ABCF1, ABCG1, ABCG2, ABCG4, ABCG5, and ABCG8.
Single-nucleotide polymorphisms (SNPs) at some gene loci are useful as markers of individual risk for adverse drug reactions or susceptibility to complex diseases. We have been focusing on identifying SNPs in and around genes encoding drug-metabolizing enzymes and transporters, and have constructed several high-density SNP maps of such regions. Here we report SNPs at additional loci, specifically 13 genes belonging to the superfamily of ATP-binding cassette transporters ( ABCA4, ABCA7, ABCA8, ABCD1, ABCD3, ABCD4, ABCE1, ABCF1, ABCG1, ABCG2, ABCG4, ABCG5, and ABCG8). Sequencing a total of 416 kb of genomic DNA from 48 Japanese volunteers identified 605 SNPs among these 13 loci: 14 in 5' flanking regions, 5 in 5' untranslated regions, 37 within coding elements, 529 in introns, 8 in 3' untranslated regions, and 12 in 3' flanking regions. By comparing our data with SNPs deposited in the dbSNP database of the National Center for Biotechnology Information (US) and with published reports, we determined that 491 (81%) of the SNPs reported here were novel. We also detected 107 genetic variations of other types among the loci examined (insertion-deletions or mono- di-, or trinucleotide polymorphisms). The high-density SNP maps we constructed on the basis of these data should provide useful information for investigating associations between genetic variations and common diseases or responsiveness to drug therapy.
Laboratory for Genotyping, RIKEN SNP Research Center, Tokyo, Japan.
Aritoshi
A
Iida
Susumu
S
Saito
Akihiro
A
Sekine
Chihiro
C
Mishima
Yuri
Y
Kitamura
Kimei
K
Kondo
Satoko
S
Harigae
Saori
S
Osawa
Yusuke
Y
Nakamura
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D002377:Catalogs as Topic; D004247:DNA / Q000097:blood / Q000302:isolation & purification; D017931:DNA Primers / Q000737:chemistry; D005091:Exons; D017353:Gene Deletion; D014644:Genetic Variation; D006801:Humans; D007438:Introns; D007564:Japan / Q000453:epidemiology; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA; D020506:Untranslated Regions
D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D020506:Untranslated Regions; D004247:DNA