Callus cultures of Annona squamosa for the production of annonaceous acetogenins.
Callus cultures of Annona squamosa were induced using different explants including petals, seed contents (megagametophyte and embryo) and fruits (mesocarp). Growth of the calli induced from the explants was found to be influenced by the type, concentration and ratio of auxin vs. cytokinin. The content of squamocin (67.8 microg g(-1) dry weight) in calli cultured on Gamborg B-5 medium containing 5.0 mg l(-1) naphthalene acetic acid and 4.0 mg l(-1) zeatin was nearly seven times higher than that in intact fruits.
Wang
Li-Da
LD
School of Pharmaceutical Sciences, Peking University, Beijing, China.
Qiu
De-You
DY
Chen
Ji-Yun
JY
Han
Yi-Fan
YF
Zheng
Jun-Hua
JH
Guo
De-An
DA
D031041:Annona; D046508:Culture Techniques; D003583:Cytokinins; D035264:Flowers / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D005663:Furans / Q000378:metabolism*; D006801:Humans; D007210:Indoleacetic Acids; D007783:Lactones / Q000378:metabolism*; D009280:Naphthaleneacetic Acids; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism; D012038:Regeneration / Q000502:physiology; D012639:Seeds / Q000737:chemistry; D015026:Zeatin
D003583:Cytokinins; D005663:Furans; D007210:Indoleacetic Acids; D007783:Lactones; D009280:Naphthaleneacetic Acids; D010936:Plant Extracts; C059660:squamocin; D015026:Zeatin