Uridine nucleotide-induced stimulation of gluconeogenesis in isolated rat proximal tubules.
Uridine nucleotides, released into the extracellular environment, influence a variety of metabolic and other cellular activities in a wide range of target tissues. Here we have studied the effects of uridine nucleotides on gluconeogenesis in isolated rat proximal tubules. Gluconeogenesis, from a range of precursors, was stimulated following exposure of isolated proximal tubules to either UTP or UTPgammaS, but not when exposed to other uridine-containing nucleotides. UTP- and UTPgammaS-induced gluconeogenesis was diminished in the presence of purinoceptor antagonists (e.g. suramin, PPADS) indicative of a role for P2Y(2)-like purinoceptors in these effects. Likewise, agents that interfere with either phospholipase C activation or intracellular Ca2+ mobilization decreased UTP- and UTPgammaS-induced stimulation of gluconeogenesis consistent with a role for these secondary messenger systems in the mechanism of action of extracellular UTP and UTPgammaS on proximal tubule metabolism.
Mo
Jun
J
Cellular Regulation and Signalling Group, School of Biological Sciences, University of Liverpool, UK.
Fisher
Michael J
MJ
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005943:Gluconeogenesis / Q000187:drug effects*; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D058914:Purinergic Antagonists; D051381:Rats; D017208:Rats, Wistar; D015290:Second Messenger Systems / Q000187:drug effects; D013268:Stimulation, Chemical; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors; D014500:Uracil Nucleotides / Q000494:pharmacology* / Q000502:physiology; D014544:Uridine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000502:physiology
D002121:Calcium Channel Blockers; D058914:Purinergic Antagonists; D014500:Uracil Nucleotides; D010738:Type C Phospholipases; D014544:Uridine Triphosphate