Expression profiling of lineage differentiation in pluripotential human embryonal carcinoma cells.
Pluripotential human embryonal carcinoma (EC) cell linesundergo differentiation programs resembling those occurring in embryonal stem cells during development. Expression profiling was performed during the terminal differentiation of the EC cell line, NTera2/Clone D1 by all-trans-retinoic acid. Time-response analysis via clustering of >12,000 human transcripts revealed distinct stages in the transition from an EC cell to neuronal progenitor cells expressing patterning markers compatible with posterior hindbrain fates followed by the appearance of immature postmitotic neurons with an evolving synaptic apparatus. Global analysis of gene expression allows monitoring cell fate and differentiation of EC cells in vitro and may provide insight into human embryonal stem cell development.
Houldsworth
Jane
J
Cell Biology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Heath
Simon C
SC
Bosl
George J
GJ
Studer
Lorenz
L
Chaganti
R S K
RS
D000970:Antineoplastic Agents / Q000494:pharmacology; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D019070:Cell Lineage; D054278:Embryonal Carcinoma Stem Cells; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000473:pathology*; D009474:Neurons / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D014212:Tretinoin / Q000494:pharmacology
D000970:Antineoplastic Agents; D008565:Membrane Proteins; D012333:RNA, Messenger; D014212:Tretinoin