Anther-specific expression of ipt gene in transgenic tobacco and its effect on plant development.
Isopentenyl transferase (ipt) gene from Agrobacterium tumefaciens T-DNA was placed under the control of a TA29 promoter which expresses specifically in anther. The chimeric TA29-ipt gene was transferred to tobacco plants. During flowering, mRNA of the ipt gene in the anthers of the transgenic plants accumulated and the level of iPA + iPs increased 3-4-fold in the leaves, petals, pistils, and stamens compared with those in the wild type plants. This cytokinin increase affected various aspects in development indicating that the alterations of endogenous cytokinin level by using anther-specific expression of the TA29-ipt gene affected morphology, floral organ systems and reproductivity of the transgenic plants.
Research Center of Plant Molecular and Developmental Biology, Institute of Botany, Chinese Academy of Sciences, Beijing, PR China. mami@ns.ibcas.ac.cn
Geng
G
Sa
Ma
M
Mi
Ye
Y
He-Chun
Li
L
Guo-Feng
D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D003583:Cytokinins / Q000378:metabolism; D035264:Flowers / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D003583:Cytokinins; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase