Allele specific PCR for the minor Histocompatibility antigen HA-2.
The hematopoietic system restricted minor Histocompatibility antigen (mHag), HA-2, is encoded by the novel human class I Myosin gene, MYO1G, located on the short arm of chromosome 7. The HA-2 encoding region is di-allelic and comprises the HLA-A2 restricted T cell epitope YIGEVLVSV (HA-2V) and its allelic counterpart YIGEVLVSM (HA-2M). We designed a sequence specific PCR (SSP) for both HA-2 alleles. The HA-2 genomic typing results were compared with the HA-2 CTL phenotyping in three families and revealed exact correlation. The mHag HA-2 SSP can be incorporated in DNA based typing protocols.
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, the Netherlands.
M
M
Wilke
J
J
Pool
E
E
Goulmy
D000483:Alleles; D002897:Chromosomes, Human, Pair 7; D005260:Female; D006801:Humans; D008297:Male; D015778:Minor Histocompatibility Antigens / Q000235:genetics*; D008969:Molecular Sequence Data; D009218:Myosins / Q000235:genetics*; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods*; D033581:Stem Cell Transplantation
C440608:MYO1G protein, human; D015778:Minor Histocompatibility Antigens; D009218:Myosins