Cholera toxin B subunit labeling in lamina II of spinal cord dorsal horn following chronic inflammation in rats.
We have investigated the effect of inflammation on the labeling pattern of cholera toxin B subunit (CTB)-conjugated horseradish peroxidase, an A-fiber marker, by an intra-sciatic nerve injection of the tracer. Following chronic inflammation in one hind paw in rats, there was substantial CTB labeling in lamina II of the spinal dorsal horn, which is normally absent. However, there was no change in the labeling pattern of wheat germ agglutinin or fluoride resistant acid phosphatase/thiamine monophosphatase, two C-fiber markers. The CTB labeling in lamina II after peripheral nerve injury has been interpreted as central sprouting of A-fibers or uptake of the tracer by injured C-fibers. Our results suggest that chronic inflammation and nerve injury may share some common mechanisms in generating allodynia and hyperalgesia.
Ma
Qing Ping
QP
Department of Anatomy and Developmental Biology, University College London, Gower Street, WC1E 6BT, London, UK. quingping_a@merck.com
Tian
Li
L
D000818:Animals; D002772:Cholera Toxin; D002908:Chronic Disease; D005620:Freund's Adjuvant; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology*; D007267:Injections; D009412:Nerve Fibers / Q000473:pathology*; D020671:Posterior Horn Cells / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve; D013194:Staining and Labeling / Q000379:methods
D005620:Freund's Adjuvant; D002772:Cholera Toxin; D006735:Horseradish Peroxidase