Expression and roles of herpesvirus entry mediators A and C in cells of oral origin.
The roles of viral glycoprotein D (gD) and cellular herpesvirus entry mediators A (HveA) and C (HveC) in herpes simplex virus entry into oral cells were determined. Studies with purified truncated forms of gD-1, HveA and HveC indicated that these molecules may be involved in herpes simplex virus entry into oral cells. Moreover, HveA was expressed similarly in primary cultures of gingival keratinocytes and fibroblasts, whereas HveC was expressed at higher levels in gingival keratinocytes, as determined by RT-PCR and immunocytochemical staining. Further analysis using immunohistochemistry demonstrated that both HveA and HveC were expressed in epithelial cells, fibroblasts and vascular endothelial cells in gingival tissues. However, only HveC was detected in nerve fibers. Also, HveA was detected throughout the epidermis, whereas HveC was pronounced in the strata basale and spinosum. In conclusion, this study characterized HveA and HveC, molecules that may participate in entry of herpes simplex virus into oral cells.
Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan.
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SL
Hung
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Cheng
Y-H
YH
Wang
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KW
Chang
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YT
Chen
D000328:Adult; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D005347:Fibroblasts / Q000201:enzymology / Q000821:virology; D005881:Gingiva / Q000166:cytology / Q000201:enzymology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000737:chemistry / Q000472:pathogenicity*; D006801:Humans; D007150:Immunohistochemistry; D007624:KB Cells / Q000821:virology; D015603:Keratinocytes / Q000201:enzymology / Q000821:virology; D009061:Mouth Mucosa / Q000166:cytology / Q000201:enzymology / Q000821:virology*; D000075983:Nectins; D012333:RNA, Messenger / Q000032:analysis; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
D015815:Cell Adhesion Molecules; D000075983:Nectins; D012333:RNA, Messenger; D018124:Receptors, Tumor Necrosis Factor; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus; C506028:TNFRSF14 protein, human; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1