Introduction of lanthanide(III) chelates to oligopeptides on solid phase.
The synthesis of oligopeptide building blocks for the introduction of nonluminescent and luminescent lanthanide(III) chelates to the oligopeptide structure on the solid phase is described. The oligopeptide conjugates synthesized were used in DELFIA-based receptor binding assay (motilin) as well as in LANCE time-resolved fluorescence quenching assay (caspase-3).
Peuralahti
Jari
J
PerkinElmer Life Sciences, Wallac Oy, P.O. Box 10, FIN-20101 Turku, Finland.
Hakala
Harri
H
Mukkala
Veli-Matti
VM
Loman
Kristiina
K
Hurskainen
Pertti
P
Mulari
Outi
O
Hovinen
Jari
J
D053148:Caspase 3; D020169:Caspases / Q000032:analysis; D002614:Chelating Agents / Q000138:chemical synthesis; D020650:Combinatorial Chemistry Techniques; D005453:Fluorescence; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008722:Methods; D009842:Oligopeptides / Q000138:chemical synthesis*; D011485:Protein Binding; D011964:Receptors, Gastrointestinal Hormone / Q000378:metabolism; D018013:Receptors, Neuropeptide / Q000378:metabolism
D002614:Chelating Agents; D028581:Lanthanoid Series Elements; D009842:Oligopeptides; D011964:Receptors, Gastrointestinal Hormone; D018013:Receptors, Neuropeptide; C050802:motilin receptor; D053148:Caspase 3; D020169:Caspases