Expression of green fluorescent protein in Rickettsia conorii.
Rickettsiae are obligate intracellular class III pathogens for which genetic manipulation has only recently been shown to be feasible. Such experiments were restricted to the typhus group rickettsiae, namely R. typhi and R. prowazekii. Here we report the first genetic manipulation of Rickettsia conorii, the bacterial agent responsible for the Mediterranean spotted fever. A gene encoding a variant of the green fluorescent protein under the control of the sterically repressed promoter (srp) from E. coli was integrated into the genome of this bacteria and detected by FACS analysis.
Unité des Rickettsies-CNRS UMR-6020, IFR 48, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385, Marseille, France. patricia.renesto@medecine.univ-mrs.fr
Patricia
P
Renesto
Edith
E
Gouin
Didier
D
Raoult
D000818:Animals; D015415:Biomarkers; D002522:Chlorocebus aethiops; D005434:Flow Cytometry / Q000379:methods; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D012281:Rickettsia / Q000145:classification / Q000235:genetics*; D018271:Signal Recognition Particle; D014169:Transformation, Bacterial; D014709:Vero Cells
D015415:Biomarkers; D008164:Luminescent Proteins; D018271:Signal Recognition Particle; D049452:Green Fluorescent Proteins