Mechanism of the antiulcerogenic effect of IL-11 on acetic acid-induced gastric ulcer in rats.
The purpose of this study was to evaluate the healing effect of interleukin-11 (IL-11) on acetic acid-induced gastric ulcer in rats. Gastric ulcers were induced in male Wistar rats by applying acetic acid to the fundus of the stomach. Recombinant human interleukin-11 (rhIL-11 100 microg/kg/twice daily, subcutaneously) was administered starting on the 2nd day before ulcer induction up through the 7th day after ulcer induction. Control rats were injected with bovine serum albumin. At 12 hours and 7 days after ulcer induction, the animals were sacrificed, and the ulcer index, proliferating cell nuclear antigen (PCNA) expression, and IL-11alpha receptor expression in the gastric tissues were studied. The ulcer index of the rhIL-11-treated rats was significantly lower than that of the control rats at the 7th day. The expression of PCNA as evaluated by Western blotting and immunohistochemistry, was enhanced in both the mucosal proliferative zone and proper muscle layer of the rhIL-11-treated rats in comparison with that in the control rats. IL-11alpha receptor expression was observed in the mucosal neck cells of the rhIL-11-treated rats and control rats. These findings suggest that IL-11 accelerates ulcer healing by inducing the proliferation of mucosal and muscular cells.
Department of Molecular Pathology, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, 1-12-4, Sakamoto, Nagasaki, 852-8523, Japan. d398008z@stcc.nagasaki-u.ac.jp
Chun Yang
CY
Wen
Masahiro
M
Ito
Mutsumi
M
Matsuu
Eiichiro
E
Fukuda
Kazuko
K
Shichijo
Masahiro
M
Nakashima
Toshiyuki
T
Nakayama
Ichiro
I
Sekine
D019342:Acetic Acid / Q000633:toxicity; D000818:Animals; D006801:Humans; D017370:Interleukin-11 / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D013270:Stomach / Q000187:drug effects / Q000473:pathology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology
D017370:Interleukin-11; D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins; D019342:Acetic Acid