Expression of platelet-derived endothelial cell growth factor/thymidine phosphorylase in cervical intraepithelial neoplasia.
Angiogenesis contributes to the growth and secondary spreading of solid tumors. Platelet-derived endothelial cell growth factor (PD-ECGF)/thymidine phosphorylase (TP) has been identified as such an angiogenic factor. In this study, the expression of PD-ECGF/TP and VEGF was evaluated by immunohistochemical staining of tumor specimens from 40 patients with cervical intraepithelial neoplasia (10 with moderate dysplasia; 10 with severe dysplasia; 10 with carcinoma in situ; 10 with invasive carcinoma). The microvessel density was assessed by immunostaining for factor VIII-related antigen in the most highly neovascularized area. In both the nucleus and cytoplasm, the intensity of PD-ECGF/TP expression in carcinoma in situ and invasive carcinoma was significantly stronger than that in moderate dysplasia. However, the intensity of VEGF expression was not significantly different in the various specimens. The microvessel density in mild dysplasia was significantly different from that in carcinoma in situ (p<0.05), and that in invasive carcinoma (p<0.05). There was no significant relationship between the microvessel density and the expression of PD-ECGF/TP or that of VEGF. These results show that the expression of PD-ECGF/TP appears to be involved in the promotion of angiogenesis in cervical intraepithelial neoplasia.
Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka 537-0025, Japan. sisaka@gyne.med.osaka-u.ac.jp
Shigeyuki
S
Isaka
Keisuke
K
Sawai
Mari
M
Tomiie
Shoji
S
Kamiura
Masayasu
M
Koyama
Chihiro
C
Azuma
Shingo
S
Ishiguro
Yuji
Y
Murata
Fumitaka
F
Saji
D002278:Carcinoma in Situ / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology; D018450:Disease Progression; D016228:Endothelial Growth Factors / Q000378:metabolism; D005169:Factor VIII / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008222:Lymphokines / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000473:pathology; D013939:Thymidine Phosphorylase / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D002578:Uterine Cervical Dysplasia / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology
D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D005169:Factor VIII; D013939:Thymidine Phosphorylase