[Involvement of E2FBP1, an ARID family member protein, in the p53 regulatory pathway].
E2FBP1, an ARID (AT-rich interaction domain) family protein, has been isolated as a protein that interacts with E2F-1, a member for the E2F family transcription factor. The closely related Bright binds to specific AT-rich DNA sequences and regulates B-cell-specific gene expression. Although Bright is specifically expressed in differentiating and matured B cells, E2FBP1 is expressed ubiquitously in a variety of tissues, suggesting that E2FBP1 plays a role in different biological processes. However, the function of E2FBP1 remains largely unknown. The present work showed that expression of E2FBP1 protein was up-regulated by forced expression of p53, and its expression levels were low, in tumor cells lacking wild-type p53. Furthermore, E2FBP1 levels were increased following DNA damage, in parallel with levels of p53 and p21 Waf1/Cip1. In addition, coimmunoprecipitation assays indicated that E2FBP1 could interact with p53 in vivo. These results suggest the possibility that E2FBP1 is involved in the p53 regulatory pathway.
Ma
Kaiwen
K
Section of Molecular Embryology, Department of Maxillofacial Biology, Division of Maxillofacial/Neck Reconstruction, Graduate School of Tokyo Medical and Dental University.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000235:genetics; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology*; D005786:Gene Expression Regulation; D016158:Genes, p53 / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D009369:Neoplasms / Q000473:pathology; D009857:Oncogenes; D011401:Promoter Regions, Genetic; D015534:Trans-Activators; D014157:Transcription Factors; D014158:Transcription, Genetic
C490798:ARID3A protein, human; C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D004268:DNA-Binding Proteins; D015534:Trans-Activators; D014157:Transcription Factors