[GATA-3 in the development of anterior chamber associated immune deviation].
OBJECTIVE
To determine the role of GATA-3 (a transcript factor that has influences on Th2 cell differentiation) in the development of anterior chamber associated immune deviation (ACAID).


METHODS
Immunohistochemistry and Western blot were used to determine the localization and relative protein levels of GATA-3 respectively at different time points after anterior chamber injection of 50 microgram interphotoreceptor retinoid binding protein (IRBP).


RESULTS
GATA-3 was detected weakly in normal spleen and increased significantly at 5, 7, 14 and 21 days after anterior chamber inoculation. It was detected mainly in CD(4)(+) T cells in spleen.


CONCLUSIONS
The expression of GATA-3 increases earlier than development of ACAID. It suggests that GATA-3 play an important role in Th2 commitment during ACAID development.
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Tao
T
Fu
Peizeng
P
Yang
Xiangkun
X
Huang
Hongyan
H
Zhou
Huahong
H
Zhong
Bing
B
Li
Hong
H
Wang
Zhen
Z
Zhang
D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005136:Eye Proteins; D050990:GATA3 Transcription Factor; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012177:Retinol-Binding Proteins / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000099214:Retinol-Binding Proteins, Interstitial
D004268:DNA-Binding Proteins; D005136:Eye Proteins; D050990:GATA3 Transcription Factor; D012177:Retinol-Binding Proteins; D015534:Trans-Activators; D000099214:Retinol-Binding Proteins, Interstitial