Metal resistance in yeast mediated by the expression of a maize 20S proteasome alpha subunit.
Transformation of yeast cells with a maize cDNA ZmPAA, encoding a 20S proteasome alpha-subunit, conferred resistance to nickel, cadmium and cobalt. This resistance is not linked to a modification of the intracellular nickel content, as no accumulation of nickel was measured between yeast cells transformed with a void vector or the ZmPAA cDNA. The abundance of the ZmPAA mRNA was increased in the shoots of maize plants upon nickel treatment. These results suggest that the proteasome might be involved in nickel resistance by scavenging metal oxidized proteins both in plants and yeast.
Laboratoire de Biochimie et Physiologie Moléculaire des Plantes, CNRS UMR 5004 Université Montpellier 2, Agro-M/INRA, Montpellier, France.
Céline
C
Forzani
Stéphane
S
Lobréaux
Stéphane
S
Mari
Jean-François
JF
Briat
Michel
M
Lebrun
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D003546:Cysteine Endopeptidases / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D015870:Gene Expression; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D009532:Nickel / Q000494:pharmacology*; D010641:Phenotype; D046988:Proteasome Endopeptidase Complex; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic; D003313:Zea mays / Q000187:drug effects / Q000235:genetics*
D018076:DNA, Complementary; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D009532:Nickel; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex