Modification of YACs by Homologous Recombination and Transfer of a 330 kb YAC into Mammalian Cells.
YAC clones were modified via homologous recombination by transfecting yeast cells containing YAC with plasmid PRAN4 DNAs. The integration of neo gene into YAC DNA was identified using Southern blotting and PCR methods. A modified YAC that carried 330 kb human DNA was introduced into L929 cells through PEG mediated spheroplast fusion, and G418 resistant colonies were obtained.
National Laboratory of Oncogenes and Related Genes, Shanghai Institute of Cancer, Shanghai 200032, China.
Gong-Yi
GY
Ren
Xin-Tai
XT
Zhao
Da-Fang
DF
Wan
Jian-Ren
JR
Gu