Extracellular matrix components are altered in the hippocampus, cortex, and cerebrospinal fluid of patients with mesial temporal lobe epilepsy.
OBJECTIVE
This work studied the profile of glycosaminoglycans (GAGs) in the hippocampus, cortex, and cerebrospinal fluid of patients with temporal lobe epilepsy (TLE).


METHODS
The GAGs were analyzed by agarose gel electrophoresis, enzymatic degradation, and enzyme-linked immunosorbent assay (ELISA).


RESULTS
The hippocampus of TLE patients showed increased levels of chondroitin sulfate and hyaluronic acid against normal levels of these GAGs in the neocortex and cerebrospinal fluid (CSF).


CONCLUSIONS
These results suggest that these matrix components could be involved in the pathophysiology of TLE.
Disciplina de Bioquímica, Universidade Federal de São Paulo, Rua Botucatu 862, 04023-900, São Paulo, SP, Brazil.
S R
SR
Perosa
M A
MA
Porcionatto
A
A
Cukiert
J R M
JR
Martins
D
D
Amado
H B
HB
Nader
E A
EA
Cavalheiro
J P
JP
Leite
M G
MG
Naffah-Mazzacoratti
D002540:Cerebral Cortex / Q000378:metabolism*; D002555:Cerebrospinal Fluid / Q000378:metabolism*; D002809:Chondroitin Sulfates / Q000378:metabolism; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism
D002809:Chondroitin Sulfates; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid