Secretion of IL-6, IL-11 and LIF by human cardiomyocytes in primary culture.
Interleukin (IL)-6-type cytokines are multifunctional proteins involved in cardiac hypertrophy and myocardial protection. Recent studies, performed on animal models, report the production of these cytokines by heart. The aim of this study was to analyse the capacity of myocytes and fibroblasts isolated from human atrium to secrete IL-6, leukaemia inhibitory factor (LIF), cardiotrophin-1 (CT-1), IL-11, oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and the soluble receptor subunits sIL-6R and sgp130 during primary culture. We detected LIF, IL-11, sgp130 and a large amount of IL-6, but not OSM, CT-1, CNTF nor IL-6R in these culture supernatants. Both cardiomyocytes and fibroblasts are able to spontaneously produce IL-6. The increase of IL-6 production all along the culture period appears to be the consequence of fibroblast proliferation and gp130 stimulation. This is the first demonstration that human cardiac cells are able to secrete IL-6, but also LIF and IL-11 in vitro. These cytokines could be involved in an autocrine and/or a paracrine networks regulating myocardial cyto-protection, hypertrophy and fibrosis.
Laboratoire des Biomembranes et Signalisation Cellulaire, UMR CNRS 6558, Université de Poitiers, France.
Cecile
C
Ancey
Pierre
P
Corbi
Josy
J
Froger
Adriana
A
Delwail
John
J
Wijdenes
Hugues
H
Gascan
Daniel
D
Potreau
Jean-Claude
JC
Lecron
D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor / Q000096:biosynthesis; D050822:Cytokine Receptor gp130; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D017370:Interleukin-11 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D007700:Kinetics; D053605:Leukemia Inhibitory Factor; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D018832:Molecular Chaperones / Q000096:biosynthesis*; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism*; D053683:Oncostatin M; D010455:Peptides / Q000378:metabolism; D011506:Proteins; D019947:Receptors, Interleukin-6 / Q000096:biosynthesis; D013997:Time Factors
D015703:Antigens, CD; D020934:Ciliary Neurotrophic Factor; D016207:Cytokines; C494199:IL6ST protein, human; D017370:Interleukin-11; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008562:Membrane Glycoproteins; D018832:Molecular Chaperones; C508351:OSM protein, human; D010455:Peptides; D011506:Proteins; D019947:Receptors, Interleukin-6; D053683:Oncostatin M; D050822:Cytokine Receptor gp130; C092073:cardiotrophin 1