Monoclonal anti-diuron antibodies prevent inhibition of photosynthesis by diuron.
Two monoclonal anti-diuron antibodies were generated that bind to diuron with an extremely low equilibrium dissociation constant. The antibodies prevented and restored in vitro and in vivo the diuron-dependent inhibition of photosynthesis. In isolated thylakoids prepared from spinach leaves (Spinacia oleracea L.) the diuron-inhibited Hill reaction was reconstituted immediately after the addition of the monoclonal antibodies. The antibodies also restored the diuron-dependent inhibition of the photosynthetic oxygen evolution of the cell wall-deficient mutant cw15 of the green alga Chlamydomonas reinhardtii Dangeard.
University of Potsdam, Institute of Biochemistry and Biology, Biotechnology, Karl-Liebknecht-Strasse 24-25, Building 25, D-14476, Golm, Germany.
Deljana
D
Werner
Olaf
O
Behrsing
Gudrun
G
Scharte
Jochen
J
Woller
Martin
M
Steup
Burkhard
B
Micheel
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D002696:Chlamydomonas / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004237:Diuron / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000494:pharmacology; D006540:Herbicides / Q000037:antagonists & inhibitors / Q000276:immunology / Q000494:pharmacology; D009154:Mutation; D010788:Photosynthesis / Q000187:drug effects* / Q000502:physiology
D000911:Antibodies, Monoclonal; D006540:Herbicides; D004237:Diuron