Melatonin in the unicellular Tetrahymena pyriformis: effects of different lighting conditions.
Melatonin content in the cellular fraction and medium of Tetrahymena pyriformis GL cultures was measured at different time points of light and dark exposures. Tetrahymena produced, stored and secreted immunoreactive melatonin, which in displacement and HPLC studies, behaved like synthetic melatonin. There was not a continuous secretion of melatonin produced by the cells. In contrast to this, storage of melatonin was observed, which was more expressed in dark conditions. Prolonged light exposure suppressed melatonin production and secretion alike, however it did not block it completely.
Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary.
L
L
Köhidai
O
O
Vakkuri
M
M
Keresztesi
J
J
Leppäluoto
G
G
Csaba
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D008027:Light; D008550:Melatonin / Q000378:metabolism / Q000494:pharmacology*; D013769:Tetrahymena pyriformis / Q000378:metabolism*; D013997:Time Factors
D008550:Melatonin