Effect of medium composition, flow rate, and signaling compounds on the formation of soluble extracellular materials by biofilms of Chromobacterium violaceum.
Biofilms of the homoserine-lactone-defective strain Chromobacterium violaceum CV026 were grown on silicone surfaces in a flow chamber. The effect of medium flow rate, different levels of N-butanoyl-homoserine lactone, and nutrients on biofilm activity was studied by quantifying the proteins and exopolysaccharides attached to the cells. To compare the effect of each of the three variables within a wide range, the experiments were designed as a full factorial search with three levels for each variable. Calculated contour plots demonstrated that N-acyl homoserine lactone is an important determinant of both the amount and the composition of the compounds excreted into the medium.
University of Zurich, Institute of Plant Biology, Zollikerstrasse 107, Switzerland.
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Martinelli
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Bachofen
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Brandl
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis*; D018441:Biofilms; D002861:Chromobacterium / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*
D001426:Bacterial Proteins; D003470:Culture Media; D011135:Polysaccharides, Bacterial; C088386:homoserine lactone; D015107:4-Butyrolactone