[Effect of pressure on proliferation and apoptosis of bovine trabecular meshwork].
OBJECTIVE
To investigate the effect of pressure on the expression of cell cycle and apoptosis related gene bcl-2 and bax mRNA by bovine trabecular meshwork cells.


METHODS
Twenty mm Hg, 40 mm Hg, 60 mm Hg and 80 mm Hg(1 mm Hg = 0.133 kPa) pressure were given to the cultured trabecular meshwork cells respectively in the treatment groups. No pressure was given in the control groups. Each pressure sustained for 24 and 48 hours. Proliferation index, apoptosis index, bcl-2 and bax mRNA expression of trabecular meshwork cells were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling, flow cytometry and in situ hybridization.


RESULTS
In 24 hour groups, there were no significant differences between the apoptosis index of control group and pressure </= 40 mm Hg groups. But in 40 mm Hg groups, the proliferative index decreased and bax mRNA expression increased significantly. The apoptosis index increased remarkably when the pressure was >/= 60 mm Hg. In 48 hour groups, apoptosis index, proliferative index and bax mRNA expression had significant differences from that of the control groups when pressure was >/= 40 mm Hg.


CONCLUSIONS
Pressure >/= 40 mm Hg sustaining for a period of time can suppress trabecular meshwork cell proliferation and induce apoptosis through influencing bcl-2 family members.
Department of Ophthalmology, Tongji Hospital of Tongji Medical College, Huazhong University of Sciense and Technology, Wuhan 430030, China. xuewei@public.wuhan.cngb.com
Wei
W
Xue
Shuhua
S
Du
Yong
Y
Li
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002417:Cattle; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D020287:In Situ Nick-End Labeling; D011312:Pressure; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014129:Trabecular Meshwork / Q000166:cytology / Q000378:metabolism*; D051028:bcl-2-Associated X Protein
D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein