Increases in amino-cupric-silver staining of the supraoptic nucleus after sleep deprivation.
Sleep deprived rats undergo a predictable sequence of physiological changes, including changes in skin condition, increased energy expenditure, and altered thermoregulation. Amino-cupric-silver staining was used to identify sleep deprivation related changes in the brain. A significant increase in staining was observed in the supraoptic nucleus (SON) of the hypothalamus of rats with high sleep loss (>45 h) vs. their yoked controls. Follow-up experiments showed that staining was not significantly different in rats sleep deprived for less than 45 h, suggesting that injurious sleep deprivation-related processes occur above a threshold quantity of sleep loss. These anatomical changes suggest that the effects of sleep deprivation may be related to protein metabolism in certain brain regions.
Eiland
Monica M
MM
University of California, Los Angeles, CA, USA.
Ramanathan
Lalini
L
Gulyani
Seema
S
Gilliland
Marcia
M
Bergmann
Bernard M
BM
Rechtschaffen
Allan
A
Siegel
Jerome M
JM
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012892:Sleep Deprivation / Q000378:metabolism*; D013194:Staining and Labeling; D013495:Supraoptic Nucleus / Q000378:metabolism*; D013997:Time Factors