[Expression of NM23-H(1) mRNA in acute leukemia].
OBJECTIVE
To determine the expression of NM23-H(1) gene in acute leukemia(AL) and evaluate the relationship between NM23-H(1) expression and clinical features.


METHODS
Expression level of NM23-H(1) mRNA in bone marrow cells was determined in 82 acute leukemia patients and 15 normal subjects with semi-quantitative reverse transcription polymerase chain reaction(RT-PCR).


RESULTS
NM23-H(1)/ GAP DH ratio >/= 0.5 was considered to be positive. NM23-H(1) mRNA was negative in all the 15 normal subjects. Expression of NM23-H(1) was positive in 43 of the 56 acute leukemia patients in the first visit, expression range being 0.33 approximately 2.75. There was one positive case in 12 AL patients with complete remission, expression range being 0 approximately 0.63,but there was no positive case in 6 AL patients who had maintained complete remission for more than 6 months, expression range being 0 approximately 0.27. Relapsed cases were all positive with an expression range of 0.76 approximately 1.87. NM23-H(1) expression in patients with initial and relapsed acute leukemia was higher than that in normal subjects (P < 0.01).


CONCLUSIONS
Overexpression of NM23-H(1) mRNA can predict treatment outcome and may be an important prognostic factor.
Department of Hematology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China.
Shaoling
S
Wu
Chunting
C
Zhao
D000293:Adolescent; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000235:genetics* / Q000503:physiopathology; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000503:physiopathology; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics* / Q000503:physiopathology; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics*; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D012333:RNA, Messenger; D012074:Remission Induction; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D054778:NM23 Nucleoside Diphosphate Kinases; D012333:RNA, Messenger; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins