Artificial regulation of transcription applying carbohydrate-lectin interaction.
A complex of plasmid DNA-lactose conjugate with lectin is proposed as an artificial system to control transcription activity: the in vitro transcription of DNA in the conjugate with T7 RNA polymerase was repressed in the presence of RCA120, and then the transcription ability was recovered by adding lactose or a lactose-carrying polymer to the repression system.
Department of Molecular Design, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan.
Kazunori
K
Matsuura
Katsuhiro
K
Hayashi
Kazukiyo
K
Kobayashi
D050260:Carbohydrate Metabolism; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D037102:Lectins / Q000378:metabolism*; D010957:Plasmids; D011485:Protein Binding; D014158:Transcription, Genetic
D037102:Lectins; D004247:DNA