Preliminary X-ray crystallographic analysis of ciliate Euplotes octocarinatus release factor eRF1a.
eRF1a, one of the class-I release factors from ciliate Euplotes octocarinatus, has been crystallized by the vapor-diffusion method using polyethylene glycol 4000 as the precipitant at pH 7.5. The crystal belongs to space group P2(1) and the unit-cell parameters are a=90.4, b=107.9, c=114.8A, beta=94.2 degrees. There appear to be four eRF1a molecules in the asymmetric unit.
Zhang
S
S
The Institute of Biotechnology, Shanxi University, Taiyuan, 030006, China.
Zhou
W
W
Ding
Y
Y
He
X
X
Zhang
H
H
An
J
J
Gao
F
F
Heckmann
K
K
Liang
A
A
Rao
Z
Z
D000818:Animals; D002923:Cilia / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D016812:Euplotes / Q000737:chemistry*; D010454:Peptide Termination Factors / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D015800:Protozoan Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism
D010454:Peptide Termination Factors; D015800:Protozoan Proteins; D011994:Recombinant Proteins