Oligodeoxynucleotides containing amide-linked LNA-type dinucleotides: synthesis and high-affinity nucleic acid hybridization.
Synthesis of three different amide-linked LNA-type dinucleotides and their incorporation into 9-mer and 17-mer oligodeoxynucleotides is described; compared to the reference DNA-RNA duplex, incorporation of one of the three dimers (5'-DNA*LNA dimer) induced significantly increased duplex thermostabilities.
Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark.
Anne
A
Lauritsen
Jesper
J
Wengel
D000577:Amides / Q000737:chemistry; D003432:Cross-Linking Reagents; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011740:Pyrimidine Dimers / Q000138:chemical synthesis* / Q000737:chemistry; D013696:Temperature
D000577:Amides; D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; D011740:Pyrimidine Dimers