Simplified high-performance liquid chromatographic determination of residual amprolium in edible chicken tissues.
A simplified determining method for the routine monitoring of residual amprolium in edible chicken tissues (muscle and liver) is developed using a high-performance liquid chromatographic (HPLC) method with a photodiode-array detector after sample cleanup by an Ultrafree-MC/PL centrifugal ultrafiltration unit. For the HPLC determination and identification, a Mightysil RP4 GP column and a mobile phase of an ethanol-5 mM 1-heptanesulfonic acid sodium salt solution (35:65, v/v) using an ion-pairing system with a photodiode-array detector are used. Average recoveries (spiked at 0.3-3.0 microg/g) are > 90%. The inter- and intravariabilities are 1.9-2.4%. The limits of quantitation are 0.22 microg/g for muscle and 0.25 microg/g for liver. The total time and solvent required for the analysis of one sample are < 20 min and < 2 mL of ethanol, respectively. No toxic solvents and regents are used.
Graduate School of Human Life Science, Osaka City University, Osaka, Japan.
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Furusawa
D000670:Amprolium / Q000032:analysis*; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003049:Coccidiostats / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D011202:Poultry Products / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003049:Coccidiostats; D000670:Amprolium