[Three-pronged approach to treat rectal cancer: liposome transfer cytosine deaminase gene, ionizing radiation and dendritic cells].
OBJECTIVE
To investigate the anticancer activity of three-pronged approach: liposome transfer cytosine deaminase (CD) gene, ionizing radiation, and dendritic cells (DCs).


METHODS
After rectal cancer cells were killed by CD and 5-FC and radiotherapy with improved liposome transfection and radiosensitization, the killed cancer cells, DCs and T lymphocytes were incubated together. The uptake of (3)H-TdR weighed the immune activity of DCs. The effect of anticancer was monitored by MTT.


RESULTS
DCs acquired the apoptosis body and other antigen from the killed cancer cells and presented them to T-lymphocytes. The induced CTL killed the cancer cells powerfully and selectively. The mortality of rectal cancer cells was 98.1%, 76.2%, 37.8% at the ratio of efficacy to target of 30:1, 15:1, 1:1, respectively.


CONCLUSIONS
The three-pronged approach may represent a powerful and safe means to selectively destroy cancer cells.
Department of General Surgery, Beijing Martial General Hospital, People's Liberation Army, Beijing 100700, China.
Shiyong
S
Li
Wenping
W
Lu
Ping
P
An
Bo
B
Yu
D003131:Combined Modality Therapy; D043525:Cytosine Deaminase; D003713:Dendritic Cells; D005720:Gamma Rays / Q000627:therapeutic use*; D015316:Genetic Therapy; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D009700:Nucleoside Deaminases / Q000235:genetics / Q000627:therapeutic use*; D011839:Radiation, Ionizing; D012004:Rectal Neoplasms / Q000401:mortality / Q000532:radiotherapy / Q000628:therapy*
D008081:Liposomes; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase