NAIP-deltaEx10-11: a novel splice variant of the apoptosis inhibitor NAIP differently expressed in drug-sensitive and multidrug-resistant HL60 leukemia cells.
Alterations of neuronal apoptosis inhibitory protein (NAIP), a member of the inhibitory of apoptosis protein (IAP) family of inhibitors of apoptosis, have been previously associated with different neurodegenerative disorders. This study indicated the existence of a novel NAIP splice variant. This isoform, NAIP-deltaEx10-11, was found in tumor cell lines of different origin and in normal adult brain. Analysis of the putative protein predicted that the NAIP variant lacks part of the third BIR domain as well as the COOH-terminal tail of regular NAIP. This might suggest that it is endowed with a reduced antiapoptotic activity. This view is supported by the fact that NAIP-deltaEx10-11 mRNA and protein were much less expressed in the multidrug- and apoptosis-resistant HL60R leukemia than in its parental counterpart HL60. The opposite occurred for regular NAIP. Overall, the NAIP transcripts might be involved in tumor resistance to chemotherapeutic agents.
Dipartimento di Scienze Farmacologiche, Università di Palermo, Via del Vespro 129, Palermo, Italy.
Monica
M
Notarbartolo
Melchiorre
M
Cervello
Luisa
L
Dusonchet
Natale
N
D'Alessandro
D017398:Alternative Splicing; D017209:Apoptosis / Q000235:genetics*; D001923:Brain Chemistry; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005091:Exons / Q000235:genetics; D018922:HL-60 Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics* / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000502:physiology; D051616:Neuronal Apoptosis-Inhibitory Protein; D020033:Protein Isoforms / Q000235:genetics* / Q000502:physiology; D017434:Protein Structure, Tertiary; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D014407:Tumor Cells, Cultured / Q000737:chemistry
C496800:NAIP protein, human; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D051616:Neuronal Apoptosis-Inhibitory Protein; D020033:Protein Isoforms; D012322:RNA Precursors; D012334:RNA, Neoplasm