Cloning and expression of human beta-defensin-2 gene in Escherichia coli.
Human beta-defensin-2 (hBD-2), a small cationic peptide, exhibits a broad range of antimicrobial activity. It has been found to play important roles in innate and adaptive immune responses against microbial invasion. For the purposes of this study, hBD-2 gene was cloned from the lesions of human condyloma acuminatum. An expression vector was constructed and transformed into E. coli. hBD-2 was expressed as a fusion protein in both the soluble and insoluble forms, which was further confirmed by western blotting analysis.
Fang
Xiangming
X
The Central Laboratory of Sir Run Run Shaw Hospital, School of Medical Science, Zhejiang University, Hangzhou 310027, The People's Republic of China.
Peng
Li
L
Xu
Zhinan
Z
Wu
Jinmin
J
Cen
Peilin
P
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000378:metabolism*; D015153:Blotting, Western; D003001:Cloning, Molecular; D003218:Condylomata Acuminata / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023083:beta-Defensins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; C106641:DEFB4A protein, human; D017931:DNA Primers; D011993:Recombinant Fusion Proteins; D023083:beta-Defensins