Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats.
OBJECTIVE
To determine whether fructose-1,6-diphosphate (FDP) pretreatment can attenuate acute lung injury induced by ischemia-reperfusion in our isolated lung model in rats.


METHODS
Randomized, controlled study.


METHODS
Animal care facility procedure room.


METHODS
Twenty-four adult male Sprague-Dawley rats each weighing 250-350 g.


METHODS
Typical acute lung injury in rats was induced successfully by 10 mins of hypoxia followed by 75 mins of ischemia and 50 mins of reperfusion. Ischemia-reperfusion significantly increased microvascular permeability as measured by the capillary filtration coefficient, lung weight gain, lung weight to body weight ratio, pulmonary arterial pressure, and protein concentration of bronchoalveolar lav-age fluid.


RESULTS
Pretreatment with FDP significantly attenuated the acute lung injury induced by ischemia-reperfusion as shown by a significant decrease in all of the assessed variables (p <.05 p <.001). The protective effect of FDP was nearly undetectable when promazine (an ecto-adenosine 5-triphosphatase inhibitor) was added before FDP pretreatment.


CONCLUSIONS
Pretreatment with FDP significantly ameliorates acute lung injury induced by ischemia-reperfusion in rats.
Department of Emergency Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China. d1204812@ndmctsgh.edu.tw
Shi-Jye
SJ
Chu
Deh-Ming
DM
Chang
David
D
Wang
Ying-Hsin
YH
Chen
Chin-Wang
CW
Hsu
Kang
K
Hsu
D000818:Animals; D001794:Blood Pressure; D002199:Capillary Permeability; D005635:Fructosediphosphates / Q000037:antagonists & inhibitors / Q000627:therapeutic use*; D007155:Immunologic Factors / Q000627:therapeutic use*; D066298:In Vitro Techniques; D008297:Male; D009929:Organ Size; D011395:Promazine / Q000494:pharmacology; D011651:Pulmonary Artery; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury; D012128:Respiratory Distress Syndrome / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology
D005635:Fructosediphosphates; D007155:Immunologic Factors; C029063:fructose-1,6-diphosphate; D011395:Promazine