Alteration of midkine expression in the ischemic brain of humans.
Midkine (MK) is a heparin-binding growth factor that occurs as a product of the retinoic acid-inducible gene. Alteration of MK expression in ischemic brain lesions was examined in humans immunohistochemically in nine patients and in two control subjects without neurological disorders. Some neurons were MK-immunopositive, but no evident MK-immunoreactivity was observed in astrocytes in brains of control subjects. In the ischemic lesions, significant elevation of MK-immunoreactivity in the astrocytes and depletion of the reactivity in neurons were seen, especially in the early period, where edema and eosinophilic neurons were prominent. On the other hand, MK-immunoreactivity was not observed in hypertrophic and fibrillary astrocytes in the later period. These findings suggest that the MK in astrocytes play some role in the repair process in the early period of the ischemic brain lesions in humans.
Wada
Manabu
M
Department of Neuropathology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, Tokyo 183-8526, Japan.
Kamata
Makiko
M
Aizu
Yoshinori
Y
Morita
Takashi
T
Hu
Jianguo
J
Oyanagi
Kiyomitsu
K
D000368:Aged; D000369:Aged, 80 and over; D000918:Antibody Specificity; D001253:Astrocytes / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D000078792:Midkine; D013194:Staining and Labeling
D002352:Carrier Proteins; D016207:Cytokines; D000078792:Midkine