Cloning and Expression of Human Manganese-superoxide Dismutase cDNA.
The cDNA encoding human manganese-superoxide dismutase was amplified from the human liver cell (L02) total RNA by RT-PCR and sequenced. The human Mn-SOD cDNA was ligated into expression vector pET-24a(+) under T7 promoter. After 5 h induction with 1 mmol/L IPTG 800 mol/L Mn(2+) human Mn-SOD was highly expressed in E.coli BL21(DE3). The protein product was up to 50% of the bacteria total protein in soluble form and had specific SOD activity.
Research Institute of Biochemistry State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai 200237 China. qsyuan@ecust.edu.cn
Hui-Juan
HJ
Shi
Hua-Jun
HJ
He
Dong-Zhi
DZ
Wei
Qin-Sheng
QS
Yuan
Zhe-Yu
ZY
Chen
Ming-Feng
MF
Li
Xiang-Fu
XF
Wu