Functional properties of the predicted helicase of porcine reproductive and respiratory syndrome virus.
Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the positive-strand RNA virus family Arteriviridae. Although considerable research has focused on this important pathogen, little is known about the function of most PRRSV proteins. To examine characteristics of putative nonstructural proteins (nsp) encoded in ORF1b, which have been identified by nucleotide similarity to domains of equine arteritis virus, defined genomic regions were cloned and expressed in the pRSET expression system. One region, nsp10, encoded a protein with a putative helicase domain and was further examined for functional helicase-like activities. PRRSV nsp10 was found to possess a thermolabile and pH-sensitive NTPase activity that was modulated by polynucleotides and to unwind dsRNA in a 5' to 3' polarity. These results provide the first evidence of the functional properties of PRRSV helicase and further support the finding that nidovirus helicases possess properties that distinguish them from other viral helicases.
Elanco Animal Health Research and Development, a Division of Eli Lilly and Company, Greenfield, IN 64140, USA. Ebautista@piadc.ars.usda.gov
Elida M
EM
Bautista
Kay S
KS
Faaberg
Dan
D
Mickelson
Edward D
ED
McGruder
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D017766:Acid Anhydride Hydrolases / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D043583:Nucleoside-Triphosphatase; D016366:Open Reading Frames; D019316:Porcine respiratory and reproductive syndrome virus / Q000201:enzymology*; D020365:RNA Helicases / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D013696:Temperature; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D017766:Acid Anhydride Hydrolases; D000251:Adenosine Triphosphatases; D043583:Nucleoside-Triphosphatase; D020365:RNA Helicases