[Involvement of E2FBP1, an ARID family member protein, in the p53 regulatory pathway].
E2FBP1, an ARID (AT-rich interaction domain) family protein, has been isolated as a protein that interacts with E2F-1, a member for the E2F family transcription factor. The closely related Bright binds to specific AT-rich DNA sequences and regulates B-cell-specific gene expression. Although Bright is specifically expressed in differentiating and matured B cells, E2FBP1 is expressed ubiquitously in a variety of tissues, suggesting that E2FBP1 plays a role in different biological processes. However, the function of E2FBP1 remains largely unknown. The present work showed that expression of E2FBP1 protein was up-regulated by forced expression of p53, and its expression levels were low, in tumor cells lacking wild-type p53. Furthermore, E2FBP1 levels were increased following DNA damage, in parallel with levels of p53 and p21 Waf1/Cip1. In addition, coimmunoprecipitation assays indicated that E2FBP1 could interact with p53 in vivo. These results suggest the possibility that E2FBP1 is involved in the p53 regulatory pathway.
Section of Molecular Embryology, Department of Maxillofacial Biology, Division of Maxillofacial/Neck Reconstruction, Graduate School of Tokyo Medical and Dental University.
Kaiwen
K
Ma
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000235:genetics; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology*; D005786:Gene Expression Regulation; D016158:Genes, p53 / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D009369:Neoplasms / Q000473:pathology; D009857:Oncogenes; D011401:Promoter Regions, Genetic; D015534:Trans-Activators; D014157:Transcription Factors; D014158:Transcription, Genetic
C490798:ARID3A protein, human; C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D004268:DNA-Binding Proteins; D015534:Trans-Activators; D014157:Transcription Factors