Silkworm hemolymph as a potent inhibitor of apoptosis in Sf9 cells.
We have previously shown that silkworm hemolymph exhibits anti-apoptotic activity against baculovirus-induced Sf9 cell apoptosis. In this study, using various chemical inducers, such as actinomycin D, camptothecin, and staurosporine, we found that silkworm hemolymph inhibits insect cell apoptosis induced not only by baculovirus but also by chemical inducers. This indicates that silkworm hemolymph contains anti-apoptotic components that work directly in insect cell apoptosis without any booster expression of baculoviral genes. With the analysis of Sf-caspase-1 activity, it was found that the inhibitory effect of silkworm hemolymph works in a further upstream step than the Sf-caspase-1 activation step.
Rhee
Won Jong
WJ
School of Chemical Engineering and Institute of Chemical Processes, Seoul National University, Kwanak-Gu Shilim-Dong San 56-1, Seoul, Republic of Korea.
Kim
Eun Jeong
EJ
Park
Tai Hyun
TH
D000818:Animals; D017209:Apoptosis; D012831:Bombyx / Q000378:metabolism*; D002166:Camptothecin / Q000494:pharmacology; D020170:Caspase 1 / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D003609:Dactinomycin / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005434:Flow Cytometry; D006458:Hemolymph / Q000378:metabolism*; D007313:Insecta; D013058:Mass Spectrometry; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D019311:Staurosporine / Q000494:pharmacology; D013997:Time Factors
D004791:Enzyme Inhibitors; D011500:Protein Synthesis Inhibitors; D003609:Dactinomycin; D020169:Caspases; D020170:Caspase 1; D019311:Staurosporine; D002166:Camptothecin