Determination of acetamiprid, imidacloprid, and nitenpyram residues in vegetables and fruits by high-performance liquid chromatography with diode-array detection.
Determination of 3 neonicotinoid insecticides, nitenpyram, imidacloprid, and acetamiprid, was studied. Vegetables and fruits were extracted with acetonitrile. The crude extract was passed through a weak anion-exchange cartridge (PSA). The effluent was subjected to silica gel cartridge. Imidacloprid and acetamiprid were eluted with 10 mL of 4:6 (v/v) acetone/hexane, followed by nitenpyram with acetone (20 mL). Pesticides were determined by HPLC with a C-18 column and diode-array detection system. Imidacloprid and acetamiprid were recovered at about 90% at the spike levels with 0.2 and 2 mg/kg in cucumber, potato, tomato, eggplant, Japanese radish, and grape. Nitenpyram was recovered at 64-80%. Relative standard deviations were less than 10% throughout all the recovery tests. In the residue analysis, agriculturally incurred pesticides at 0.08-0.14 mg/kg were designated with UV spectra compared with respective reference standards.
Obana
Hirotaka
H
Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari, Osaka, 5370025 Japan. obana@iph.pref.osaka.jp
Okihashi
Msahiro
M
Akutsu
Kazuhiko
K
Kitagawa
Yoko
Y
Hori
Shinjiro
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D007093:Imidazoles / Q000032:analysis; D007306:Insecticides / Q000032:analysis*; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010573:Pesticide Residues / Q000032:analysis*; D011725:Pyridines / Q000032:analysis; D014675:Vegetables / Q000737:chemistry*
D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010573:Pesticide Residues; D011725:Pyridines; C464843:nitenpyram; C082359:imidacloprid; C464485:acetamiprid