Engineered biosynthesis of novel spinosyns bearing altered deoxyhexose substituents.
Novel spinosyns have been prepared by biotransformation, using a genetically engineered strain of Saccharopolyspora erythraea, in which the beta-D-forosamine moiety in glycosidic linkage to the hydroxy group at C17 is replaced by alpha-L-mycarose.
Gaisser
Sabine
S
Biotica Technology Ltd, 181A Huntingdon Road, Cambridge, UK CB3 0DJ.
Martin
Christine J
CJ
Wilkinson
Barrie
B
Sheridan
Rose M
RM
Lill
Rachel E
RE
Weston
Alison J
AJ
Ready
Sarah J
SJ
Waldron
Clive
C
Crouse
Gary D
GD
Leadlay
Peter F
PF
Staunton
James
J
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001711:Biotransformation; D003837:Deoxy Sugars / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D016695:Glycosyltransferases / Q000235:genetics; D006601:Hexoses / Q000378:metabolism; D018942:Macrolides; D016930:Saccharopolyspora / Q000235:genetics
D000900:Anti-Bacterial Agents; D003837:Deoxy Sugars; D006601:Hexoses; D018942:Macrolides; D016695:Glycosyltransferases