Malaria species and Southeast Asian ovalocytosis defined by a 27-bp deletion in the erythrocyte band 3 gene.
To evaluate the resistance of SAO against species specific malaria infection, relationships between parasite species and the 27-bp deletion in the band 3 gene were studied in malaria endemic Sumba Island, eastern Indonesia. Thick blood films were prepared from patients with malaria symptoms (n=129) and healthy controls (n=231). Species of Plasmodium was identified by microscopic observation. The 27-bp deletion was screened by the PCR method. Among 231 healthy controls, 29 (12.6%) had the 27-bp deletion, whereas 14 (10.9%) among 129 patients confirmed with malaria infection harbored the 27-bp deletion. No significant difference was observed in the prevalence of the 27-bp deletion between controls and patients (p>0.8). There was no significant difference in the frequency of the 27-bp deletion between P. vivax and P. falciparum infected subjects at 5% level by Fisher's exact test. The present result showing no correlation between the presence of the 27-bp deletion and infected parasite species is consistent with the post-invasion resistance hypothesis that may involve not a single malaria species.
Department of Biological Sciences, School of Science, University of Tokyo, Hongo, Japan.
Masako
M
Kimura
Augustinus
A
Soemantri
Takafumi
T
Ishida
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000235:genetics*; D001210:Asia, Southeastern; D016022:Case-Control Studies; D004612:Elliptocytosis, Hereditary / Q000235:genetics*; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D008288:Malaria / Q000097:blood* / Q000453:epidemiology / Q000469:parasitology; D010961:Plasmodium / Q000145:classification* / Q000235:genetics; D017384:Sequence Deletion
D001457:Anion Exchange Protein 1, Erythrocyte