Peptide dendrimers based on polyproline helices.
We present a new family of peptide dendrimers based on polyproline helices and cis-4-amino-L-proline as a branching unit. Dendrimers were synthesized by a convergent solid-phase peptide synthesis approach. The conformational transition between polyproline type I helix and polyproline type II helix was observed by circular dichroism in branched polyproline building blocks with more than 14 proline residues and in the resulting dendrimers. Both linear and dendritic polyprolines were found to be actively internalized by rat kidney cells. Preliminary results show that the antibiotic ciprofloxacin form complexes with branched polyproline chains in 99.5% propanol.
Crespo
Laia
L
Departament de Química Orgànica, Universitat de Barcelona, Martí i Franquès 1, 08028-Barcelona, Spain.
Sanclimens
Glòria
G
Montaner
Beatriz
B
Pérez-Tomás
Ricardo
R
Royo
Miriam
M
Pons
Miquel
M
Albericio
Fernando
F
Giralt
Ernest
E
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry; D002460:Cell Line; D002939:Ciprofloxacin / Q000737:chemistry; D002942:Circular Dichroism; D007668:Kidney / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D017433:Protein Structure, Secondary; D051381:Rats
D000890:Anti-Infective Agents; D010455:Peptides; C011083:polyproline; D002939:Ciprofloxacin