Stereoselective protein binding of alprenolol in the renal diseased state.
The investigation was undertaken to study the stereoselective protein binding of alprenolol in renal disease patient sera, compared to that in the sera of healthy volunteers. The in vitro stereoselective protein binding of beta-blockers was determined in undiluted serum and in isolated alpha(1)-acid glycoprotein (AGP) solutions by ultrafiltration. The stereoselctive serum protein binding of alprenolol, a beta-adrenergic blocking agent, in healthy volunteers was significantly altered in renal disease patients. We investigated the effects of AGP concentration and endogenous substances, including uremic toxins, on the stereoselective protein binding of alprenolol in renal disease patients. A good correlation between the unbound (R)/(S) ratio (F(R)/F(S) ratio), an apparent index of stereoselectivity in alprenolol serum binding and AGP concentration in serum, was found. However, stereoselective protein binding was not influenced by endogenous substances. This result can be explained by the difference in binding affinities of (R) and (S)-isomers of alprenolol to AGP. We conclude that the stereoselective protein binding of alprenolol in healthy volunteers and renal disease patients varies as a result of changes in AGP concentration. Accordingly, these findings might be useful in alprenolol therapy in renal disease patients.
Faculty of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan.
Hitoshi
H
Imamura
Takafumi
T
Komori
Ahmed
A
Ismail
Ayaka
A
Suenaga
Masaki
M
Otagiri
D000526:Alprenolol / Q000097:blood / Q000737:chemistry*; D000959:Antihypertensive Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007674:Kidney Diseases / Q000097:blood* / Q000628:therapy; D009961:Orosomucoid / Q000737:chemistry* / Q000302:isolation & purification; D011485:Protein Binding; D013237:Stereoisomerism
D000959:Antihypertensive Agents; D009961:Orosomucoid; D000526:Alprenolol