Renal cell carcinoma related novel gene, GYLZ-RCC18: cloning and functional studies.
OBJECTIVE
To clone the full length of renal cell carcinoma (RCC) related novel gene GYLZ-RCC18 and study its function.


METHODS
SMART RACE technology was used to clone the full length of GYLZ-RCC18. RT-PCR was used to detect its expression in renal cell carcinoma tissue at different stages and grades. We transfected the antisense oligonucleotide of GYLZ-RCC18 to renal cell carcinoma cell line, GRC-1, and analyzed proliferation activity, growth rate, apoptosis, and mortality changes.


RESULTS
The full length of GYLZ-RCC18 (GenBank accession number: BE825133) cDNA was about 3.5 kb. GYLZ-RCC18 had a higher expression in higher grades and stages of renal cell carcinoma than in lower ones. The expression of GYLZ-RCC18 in renal cell carcinoma was much higher than in normal kidney. After the transfection of GYLZ-RCC18 antisense oligonucleotide, the mortality of GRC-1 increased significantly, while proliferative activity and growth rate were substantially inhibited at the same time. The antisense oligonucleotide induced apoptosis of GRC-1 through the entire observation time.


CONCLUSIONS
GYLZ-RCC18 is an important novel gene related to renal cell carcinoma. Overexpression of this gene results in higher growth and proliferative activity and has an antiapoptosis effect on renal cell carcinoma cells. Transfection of the antisense oligonucleotide may inhibit the generation and development of renal cell carcinoma.
Zhang
Qiang
Q
Department of Urology and Institute of Urology, The First Affiliated Hospital, Peking University, Beijing 100034, China. zhanqian@public3.bta.com
Chen
Lin
L
Liang
Lili
L
Xi
Zhijun
Z
Ding
Yi
Y
Tong
Ming
M
Zhang
Zhiwen
Z
Lee
Chung
C
Guo
Yinlu
Y
D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D003001:Cloning, Molecular; D016373:DNA, Antisense / Q000235:genetics / Q000502:physiology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm / Q000235:genetics*; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics*; D009841:Oligonucleotides / Q000235:genetics; D017422:Sequence Analysis, DNA; D014162:Transfection; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense; D018076:DNA, Complementary; C462819:GYLZ-RCC18 protein, human; D009363:Neoplasm Proteins; D009841:Oligonucleotides