Pheno- and genotypic properties of streptococci of serological group B of canine and feline origin.
In the present study streptococci of serological group B isolated from canines (n=48) and felines (n=7) were comparatively investigated with group B streptococci from humans and bovines for cultural, biochemical and serological properties for antibiotic resistancies and by molecular analysis. An identification was performed with group B-specific antiserum, biochemical reactions, by PCR amplification and subsequent endonuclease digestion of the 16S rRNA gene and by amplification of species-specific parts of the 16S rDNA the 16S-23S rDNA intergenic spacer region and the CAMP factor gene cfb. Phenotypic similarities of group B streptococci of canine and feline origin with group B streptococci from humans and differences to group B streptococci of bovine origin could be observed in lactose fermentation, serotype patterns, pigmentation, growth properties of the bacteria in fluid medium and soft agar, hemagglutination reactions and in minocycline and tetracycline resistance. A negative hyaluronidase plate test, a hylB amplicon with a size of 4.6 kb and an insertion sequence 1548 could be observed among canine, feline and human group B streptococci of serotype III. The remaining hyaluronidase positive strains, also including all isolates of bovine origin, had a hylB gene with a size of 3.3 kb. Further genotypic differences could be observed in the occurrence of the genes lmb and scpB which appeared generally among canine, feline and human group B streptococci, but less pronounced among bovine isolates of this species. According to the presented data group B streptococci of canine and feline origin seemed to be more related to human than to bovine isolates of this species possibly indicating some epidemiological relation.
Yildirim
Ali Onder
AO
Institut für Tierärztliche Nahrungsmittelkunde, Professur für Milchwissenschaften der Justus-Liebig-Universität Giessen, Ludwigstr. 21, Germany.
Lämmler
Christoph
C
Weiss
Reinhard
R
Kopp
Peter
P
D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D002415:Cats; D002417:Cattle; D004285:Dogs; D024881:Drug Resistance, Bacterial; D010450:Endopeptidases / Q000235:genetics; D005285:Fermentation; D005838:Genotype; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000235:genetics; D007785:Lactose / Q000378:metabolism; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D013292:Streptococcus agalactiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D018829:Adhesins, Bacterial; D012336:RNA, Ribosomal, 16S; D006821:Hyaluronoglucosaminidase; D010450:Endopeptidases; C053088:C5a peptidase; D007785:Lactose