Combined genome and proteome approach to identify new susceptibility genes.
Type 1 diabetes mellitus (T1DM) is a multifactorial disorder characterized by a specific destruction of the insulin-producing beta cells in the islets of Langerhans. Cells from the immune system infiltrate the islet during the pathogenesis, releasing a mixture of cytokines demonstrated to be specifically toxic to the beta cells within the islets. The goal is to understand the molecular mechanisms responsible for this specific beta-cell toxicity, which will allow the design of novel intervention strategies for T1DM. The proteome approach provides a detailed picture of the beta-cell proteins changing expression pattern during cytokine-mediated beta-cell destruction. Combining the information from this proteome approach with genetic studies makes us believe that it is possible to reach this goal.
Steno Diabetes Center, Gentofte, Denmark. fpoc@novo.dk
Flemming
F
Pociot
Allan E
AE
Karlsen
D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000503:physiopathology; D015180:Electrophoresis, Gel, Two-Dimensional; D020022:Genetic Predisposition to Disease; D016678:Genome; D006801:Humans; D007515:Islets of Langerhans / Q000503:physiopathology; D020543:Proteome
D020543:Proteome