[Apoptosis of bovine trabecular meshwork cells induced by dexamethasone].
OBJECTIVE
To investigate apoptosis of bovine trabecular meshwork cells induced by dexamethasone.


METHODS
In the incubation method of system in vitro, dexamethasone (1 - 500 mg/L) was added into the syncretic culture solution of the third to fifth generation of bovine trabecular meshwork cells. After 1 - 14 days, the culture solution was observed by phase-contrast microscopy, fluorescence microscopy and transmission electron microscopy, and was studied by DNA laddering and flow cytometric analysis.


RESULTS
Dexamethasone (125 - 500 mg/L) induced apoptosis of trabecular meshwork cells in a dose-time-dependent manner.


CONCLUSIONS
Apoptosis of cultured bovine trabecular meshwork cells can be induced by dexamethasone, which may be one of the pathogenic mechanisms of steroid-induced glaucoma.
Department of Pharmacology, Sun Yat-sen University, Guangzhou 510089, China. guyajuan@163.net
Yajuan
Y
Gu
Shujun
S
Zeng
Pengxin
P
Qiu
Dawei
D
Peng
Guangmei
G
Yan
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000502:physiology*; D002417:Cattle; D053938:DNA Fragmentation / Q000187:drug effects / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology*; D005434:Flow Cytometry; D014129:Trabecular Meshwork / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D003907:Dexamethasone