Molecular mediators of Porphyromonas gingivalis-induced T-cell apoptosis.
Porphyromonas gingivalis produces virulence factors which can modify the molecular and cellular components of the host immune response. In the present work we investigated the role of specific virulence factors from P. gingivalis in the induction of apoptosis in Jurkat T cells. P. gingivalis culture supernatants mimicked the effect of butyric acid on T-cell apoptosis and this effect was associated with an increase in histone H4 acetylation. A role for proteases was excluded in experiments which demonstrated that neither protease inhibitors nor use of P. gingivalis mutants defective in protease synthesis had any effect on the stimulation of T-cell apoptosis in this system.
Department of Oral Biology, The Dental School, University of Newcastle upon Tyne, Newcastle upon Tyne, UK.
J I
JI
Harris
R R B
RR
Russell
M A
MA
Curtis
J
J
Aduse-Opoku
J J
JJ
Taylor
D000107:Acetylation; D017209:Apoptosis / Q000187:drug effects; D020148:Butyric Acid / Q000378:metabolism; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006657:Histones / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000201:enzymology / Q000472:pathogenicity*; D013601:T-Lymphocytes / Q000382:microbiology* / Q000473:pathology; D037521:Virulence Factors / Q000494:pharmacology / Q000502:physiology*
D017077:Culture Media, Conditioned; D006657:Histones; D037521:Virulence Factors; D020148:Butyric Acid; D010450:Endopeptidases; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases