Telomere Shortening of MCF-7 Cells Caused by Antisense Telomerase cDNA.
pAdE(1)CMVITREXneo is a novel adenovirus vector which can integrate foreign genes into genome of host cells. The cDNA of human telomerase RNA was integrated inversely into the vector via antisense oligonucleotide technique. Antisense recombinant virus (vAdT-AAV) was obtained by cotransfecting pAdT and pBHG(11) into 293 cells. The telomere length of breast cancer MCF-7 cells was found shortened after the transfection by vAdT-AAV.
Zhang
Xiao-Wei
XW
Department of Biochemistry and Molecular Biology, Beijing Medical University, Beijing 100083, China. biochem@mail.bjmu.edu.cn
Liao
Hai-Ni
HN
Tong
Tan-Jun
TJ