Get effective polyclonal antisera in one month.
According to the traditional immunization procedure, after the first injection of the sample A (emulsion of aimed antigen and Freund's complete adjuvant) to immunize rabbit, successive injections of the sample B (emulsion of aimed antigen and Freund's incomplete adjuvant) were followed every 2-4 weeks. In general, high titer of the corresponding polyclonal antisera will be observed after 4-5 injections of sample B in 3-4 months. This report presents a simply modified procedure that was able to stimulate the antisera formation in one month and achieve enough avidity to satisfy either Western blot or immunohistochemistry analysis. It just applied an additional injection of the sample A to the rabbit at the 3rd day after the primary immunization injection. You could gain the high titer of the antisera right after the first sample B injection in one month. This method has produced the desired results in three different recombinant antigens with different molecular weight (5.9 KD-55 KD) expressed from prokaryotic or eukaryotic cells.
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.
Yuan Xin
YX
Hu
Ju Yuan
JY
Guo
Lu
L
Shen
Yan
Y
Chen
Zu Chuan
ZC
Zhang
Yong Lian
YL
Zhang
D000818:Animals; D000917:Antibody Formation; D015153:Blotting, Western; D004655:Emulsions / Q000494:pharmacology*; D005620:Freund's Adjuvant / Q000494:pharmacology; D007106:Immune Sera / Q000096:biosynthesis*; D007150:Immunohistochemistry; D007158:Immunologic Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors
D004655:Emulsions; D007106:Immune Sera; D012710:Serum Albumin, Bovine; D005620:Freund's Adjuvant