The role of the peripheral benzodiazepine receptor in photodynamic activity of certain pyropheophorbide ether photosensitizers: albumin site II as a surrogate marker for activity.
A study has been carried out to define the importance of the peripheral benzodiazepine receptor (PBR) as a binding site for a series of chlorin-type photosensitizers, pyropheophorbide-a ethers, the subject of a previous quantitative structure-activity relationship study by us. The effects of the PBR ligand PK11195 on the photodynamic activity have been determined in vivo for certain members of this series of alkyl-substituted ethers: two of the most active derivatives (hexyl and heptyl), the least active derivative (dodecyl [C12]) and one of intermediate activity (octyl [C8]). The photodynamic therapy (PDT) effect was inhibited by PK11195 for both of the most active derivatives, but no effect on PDT activity was found for the less active C12 or C8 ethers. The inhibitory effects of PK11195 were predicted by the binding of only the active derivatives to the benzodiazepine site on albumin, ie. human serum albumin (HSA)-Site II. Thus, as with certain other types of photosensitizers, it has been demonstrated with this series of pyropheophorbide ethers that in vitro binding to HSA-Site II is a predictor of both optimal in vivo activity and binding to the PBR in vivo.
Photodynamic Therapy Center, Roswell Park Cancer Institute, Buffalo, NY 14263, USA. thomas.dougherty@roswellpark.org
Thomas J
TJ
Dougherty
Adam B
AB
Sumlin
William R
WR
Greco
Kenneth R
KR
Weishaupt
Lurine A
LA
Vaughan
Ravindra K
RK
Pandey
D000818:Animals; D001665:Binding Sites; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000627:therapeutic use; D006801:Humans; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000627:therapeutic use; D011963:Receptors, GABA-A / Q000378:metabolism*; D012513:Sarcoma, Experimental / Q000188:drug therapy / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism
D007546:Isoquinolines; D017319:Photosensitizing Agents; D011963:Receptors, GABA-A; D012709:Serum Albumin; D002734:Chlorophyll; C040298:pyropheophorbide a; C037850:PK 11195