Interactions between human platelets and eosinophils are mediated by selectin-P.
Mechanisms and biochemical consequences of platelet-neutrophil interactions are well known. In contrast, platelet-eosinophil interactions remain largely unexplored. The aim of this study was to assess whether platelets adhere to eosinophils, and to analyze whether selectin-P would mediate that phenomenon. Eosinophils and platelets were obtained from peripheral blood of healthy volunteers. Eosinophils were isolated using magnetic cell separation method. Platelets were isolated and washed. A number of "rosettes" (an eosinophil with more than 5 adherent platelets) per 100 eosinophils was examined in the eosinophil-platelet suspension. Addition of thrombin stimulated formation of "rosettes". Monoclonal antibodies against selectin-P almost completely prevented thrombin-stimulated formation of "rosettes". In summary, intercellular interaction between platelets and eosinophils are mediated by selectin-P. This phenomenon may be of importance in asthma and other atopic diseases.
Department of Pharmacology, Jagiellonian University School of Medicine, Kraków, Poland.
Jacek
J
Jawień
Stefan
S
Chłopicki
Ryszard J
RJ
Gryglewski
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001792:Blood Platelets / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D004804:Eosinophils / Q000502:physiology*; D006801:Humans; D019007:P-Selectin / Q000502:physiology*; D010973:Platelet Adhesiveness / Q000187:drug effects; D012397:Rosette Formation; D013917:Thrombin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019007:P-Selectin; D013917:Thrombin