Cytotoxic effect of nitric oxide on human hematological malignant cells.
We investigated the cytotoxic effect of nitric oxide (NO) on primary culture of human hematological malignant cells. Sodium nitroprusside (SNP), an NO donor, had cytotoxic effects on the cells of some patients with malignant lymphoma (ML), acute myelocytic leukemia (AML) or chronic myelomonocytic leukemia (CMMoL), but not with multiple myeloma. Cultured cells from the ML patient remained sensitive to SNP after the cells became resistant to anti-cancer drugs. In contrast, the cells from the patients with AML and CMMoL became resistant to SNP while anti-cancer drugs remained effective. In samples of the cells of the patients with ML and AML, the number of CD3 positive lymphoma cell was decreased by SNP and the number of CD33 negative cells and normal B lymphocytes (CD19 positive cells) were increased. In the cells of the patient with ML, apoptosis was induced by SNP. SNP had no effect on lymphocytes of healthy volunteers. These results suggest that SNP had an anti-tumor effect on human hematological malignant cells.
Tsumori
Michihiro
M
Department of Medicine, Shimane Medical University, Izumo, Japan. mtsumori@shimane-med.ac.jp
Tanaka
Junko
J
Koshimura
Kunio
K
Kawaguchi
Mikiko
M
Murakami
Yoshio
Y
Kato
Yuzuru
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007951:Leukemia, Myeloid / Q000188:drug therapy*; D008223:Lymphoma / Q000188:drug therapy*; D008297:Male; D009569:Nitric Oxide / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D013778:Tetrazolium Salts; D013844:Thiazoles
D000970:Antineoplastic Agents; D020030:Nitric Oxide Donors; D013778:Tetrazolium Salts; D013844:Thiazoles; D009599:Nitroprusside; D009569:Nitric Oxide; C022616:thiazolyl blue