[The reversion of Pichia guilliermondii transformants to the wild-type phenotype].
Pichia guilliermondii strain with blocked GTP cyclohydrolase II was transformed using replicative plasmids and their fragments containing the structural gene RIB7 of this enzyme. Experiments showed that the presence of an ARS element and the promoter region of the gene in the genome of transformants reduces the probability of their reversion to the wild-type phenotype. Different types of recombination in the yeast P. guilliermondii are discussed.
Piniaga
Iu V
IuV
Institute of Cell Biology, National Academy of Sciences of Ukraine, ul. Dragomanova 14/16, Lviv, 79005 Ukraine. yuriypinuaga@yahoo.com
Prokopiv
T M
TM
Petrishin
A V
AV
Khalimonchuk
O V
OV
Protchenko
O V
OV
Fedorovich
D V
DV
Boretskiĭ
Iu R
IuR
D006136:GTP Cyclohydrolase / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D010843:Pichia / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic
D006136:GTP Cyclohydrolase