Comparison of fresh and cryopreserved porcine ventral mesencephalon cells transplanted in A rat model of Parkinson's disease.
To evaluate whether cryopreservation of porcine ventral mesencephalon cells influences graft survival and function in vivo, we have transplanted either freshly prepared or cryopreserved cells into the striatum of 6-hydroxydopamine-lesioned rats. A single cell suspension of porcine ventral mesencephalon cells from the same isolation either was stored at 4 degrees C and transplanted the next day or was cryopreserved for 4 weeks in liquid nitrogen vapor. The cryopreserved cells were then rapidly thawed, rinsed, and transplanted in the same manner as the fresh cells, with the same dose of viable cells. All animals received daily injections of cyclosporin A to prevent xenograft rejection. To monitor graft function, amphetamine-induced rotation was measured every 3 weeks between 6 and 15 weeks posttransplantation. After sacrifice at 15 weeks posttransplantation, histological methods were used to compare fresh cell and cryopreserved cell transplants with respect to graft survival, differentiation and integration, and host immune response. Cryopreserved cells were found to be either equivalent or in some cases superior to fresh cells with respect to rotational correction, graft survival, graft volume, numbers of graft-derived dopaminergic neurons, and host immune responses. In conclusion, the results indicate that it is feasible to cryopreserve porcine ventral mesencephalon cells for long-term storage of cells prior to transplantation in an animal model of Parkinson's disease.
Department of Cell Transplantation, Diacrin Inc., Charlestown, Massachusetts, USA. djacoby@diacrin.com
Douglas B
DB
Jacoby
Charles
C
Lindberg
Judson
J
Ratliff
Kristie
K
Wetzel
Gregory R
GR
Stewart
Jonathan
J
Dinsmore
D018663:Adrenergic Agents; D000818:Animals; D001522:Behavior, Animal; D018929:Cell Culture Techniques; D003342:Corpus Striatum / Q000276:immunology / Q000601:surgery*; D015925:Cryopreservation / Q000379:methods; D005260:Female; D006085:Graft Survival; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000166:cytology / Q000637:transplantation*; D023421:Models, Animal; D016627:Oxidopamine; D010300:Parkinson Disease / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D010302:Parkinson Disease, Secondary / Q000139:chemically induced; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D013997:Time Factors; D019737:Transplants
D018663:Adrenergic Agents; D016627:Oxidopamine