In vitro cultivation of cells from ovotestis tissue of pigmented Biomphalaria glabrata.
Cells derived from ovotestis tissue of pigmented Biomphalaria glabrata, Puerto Rican strain were cultured in double diluted GIT medium supplemented with modification of amino acids components of pigmented B. glabrata, ovotestis and mid-gut region and 3% inactivated fetal calf serum. As a result, two types of cells, epithelial and fibroblastic like cells increased in number during the cultivation. It seem that the medium used in this study is a suitable medium for cultivation of cells from ovotestis of pigemeted B. glabrata. These two types of cells have been maintained by successive transplantation for over 3 passages.
Iwanaga
Yuzuru
Y
Department of Immunology and Parasitology, Hiroshima University School of Medicine, Japan. yiwanaga@hiroshima-u.ac.jp
D000818:Animals; D001700:Biomphalaria / Q000145:classification / Q000166:cytology* / Q000254:growth & development; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D003470:Culture Media / Q000737:chemistry; D004847:Epithelial Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D006066:Gonads / Q000166:cytology*; D007422:Intestines / Q000166:cytology; D010858:Pigmentation; D011647:Puerto Rico
D003470:Culture Media