A molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus, Gigaspora margarita.
To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnostic sequence from both DNA preparations of single spores and colonized roots. This protocol enabled us to distinguish the isolate among several isolates from Japan, Nepal and the USA.
Yokoyama
Kazuhira
K
Department of Biological Science, Faculty of Agriculture, Yamaguchi University, 1677-1, Yoshida, Japan. wahei@agr.yamaguchi-u.ac.jp
Tateishi
Takahiro
T
Marumoto
Takuya
T
Saito
Masanori
M
D005658:Fungi / Q000235:genetics* / Q000302:isolation & purification*; D005800:Genes, Fungal / Q000235:genetics; D005819:Genetic Markers; D015345:Oligonucleotide Probes; D019697:Onions / Q000382:microbiology; D013172:Spores, Fungal / Q000235:genetics*; D013559:Symbiosis; D014197:Trees / Q000382:microbiology
D005819:Genetic Markers; D015345:Oligonucleotide Probes