Avian papillomaviruses: the parrot Psittacus erithacus papillomavirus (PePV) genome has a unique organization of the early protein region and is phylogenetically related to the chaffinch papillomavirus.
BACKGROUND
An avian papillomavirus genome has been cloned from a cutaneous exophytic papilloma from an African grey parrot (Psittacus erithacus). The nucleotide sequence, genome organization, and phylogenetic position of the Psittacus erithacus papillomavirus (PePV) were determined. This PePV sequence represents the first complete avian papillomavirus genome defined.


RESULTS
The PePV genome (7304 basepairs) differs from other papillomaviruses, in that it has a unique organization of the early protein region lacking classical E6 and E7 open reading frames. Phylogenetic comparison of the PePV sequence with partial E1 and L1 sequences of the chaffinch (Fringilla coelebs) papillomavirus (FPV) reveals that these two avian papillomaviruses form a monophyletic cluster with a common branch that originates near the unresolved center of the papillomavirus evolutionary tree.


CONCLUSIONS
The PePV genome has a unique layout of the early protein region which represents a novel prototypic genomic organization for avian papillomaviruses. The close relationship between PePV and FPV, and between their Psittaciformes and Passeriformes hosts, supports the hypothesis that papillomaviruses have co-evolved and speciated together with their host species throughout evolution.
Tachezy
Ruth
R
Department of Experimental Virology, Instittute of Hematology and Blood Transfusion, Prague, Czech Republic. rutach@ukht.cz
Rector
Annabel
A
Havelkova
Marta
M
Wollants
Elke
E
Fiten
Pierre
P
Opdenakker
Ghislain
G
Jenson
Bennett
B
Sundberg
John
J
Van Ranst
Marc
M
D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral; D017874:Immediate-Early Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000235:genetics; D010311:Parrots / Q000821:virology*; D010802:Phylogeny; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA; D020308:Songbirds / Q000821:virology; D014412:Tumor Virus Infections / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
D020121:5' Untranslated Regions; D004279:DNA, Viral; D017874:Immediate-Early Proteins; D015678:Viral Structural Proteins