Site-directed mutagenesis by the megaprimer PCR method: variations on a theme for simultaneous introduction of multiple mutations.
Angelaccio
Sebastiana
S
Dipartimento di Scienze Biochimiche A. Rossi Fanelli, Università degli Studi di Roma La Sapienza, P.le Aldo Moro 5, 00185, Rome, Italy.
Bonaccorsi di Patti
Maria Carmela
MC
D002570:Ceruloplasmin / Q000235:genetics; D017931:DNA Primers; D004926:Escherichia coli; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins
D017931:DNA Primers; D029701:Saccharomyces cerevisiae Proteins; D002570:Ceruloplasmin; C085513:FET3 protein, S cerevisiae