FGF10 is a mesenchymally derived stimulator for epidermal development in the chick embryonic skin.
The development of avian cutaneous appendages, feathers and scales, is known to arise from the epithelial-mesenchymal interaction. Here we show that FGF10 is associated with this developmental process as an early signal from mesenchymal cells underlying nascent cutaneous placodes. Expression of Fgf10 was detected in the mesenchymal cells underneath the developing placodes. Forced expression of Fgf10 in the femoral skin suppressed expression of Shh and a zinc finger gene snail-related (cSnR), while induced expression of Bmp2 in the interbud region, resulting in thickening of the epidermal layer. Furthermore, forced expression of Fgf10 in the foot skin caused marked ingrowings of the epidermis. The cells in the epidermal ingrowings expressed beta-catenin, proliferating cell nuclear antigen, and an epidermal stem cell marker p63. These results support the idea that FGF10 is a mesenchymally derived stimulator of epidermal development through crosstalk with bone morphogenetic protein (BMP), beta-catenin, and other signaling pathways.
Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, 2-1 Minami-Jyosanjima-cho, Japan.
Hirotaka
H
Tao
Yasuko
Y
Yoshimoto
Hidefumi
H
Yoshioka
Tsutomu
T
Nohno
Sumihare
S
Noji
Hideyo
H
Ohuchi
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D002642:Chick Embryo; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D005241:Feathers / Q000196:embryology / Q000378:metabolism; D051526:Fibroblast Growth Factor 10; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D017403:In Situ Hybridization; D008565:Membrane Proteins; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D008954:Models, Biological; D010750:Phosphoproteins / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics; D051497:Receptor, Fibroblast Growth Factor, Type 2; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics; D015398:Signal Transduction; D012867:Skin / Q000196:embryology* / Q000378:metabolism; D000071250:Snail Family Transcription Factors; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta; D051176:beta Catenin
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; C081876:CKAP4 protein, human; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D051526:Fibroblast Growth Factor 10; D053823:Hedgehog Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D018809:Proliferating Cell Nuclear Antigen; D017468:Receptors, Fibroblast Growth Factor; C509239:SHH protein, human; D000071250:Snail Family Transcription Factors; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; D051176:beta Catenin; C104481:snail-related protein, Gallus gallus; D005346:Fibroblast Growth Factors; D020794:Receptor Protein-Tyrosine Kinases; D051497:Receptor, Fibroblast Growth Factor, Type 2