Establishment and characterization of a new mantle cell lymphoma cell line, Mino.
Mantle cell lymphoma (MCL) is a distinct type of B-cell non-Hodgkin's lymphoma characterized by cyclin D1 overexpression and the cytogenetic abnormality, the t(11;14)(q13;q32). MCL cell lines have been difficult to establish and in vitro studies of these neoplasms are scarce. We describe the establishment and characteristics of a new MCL cell line, Mino. The cells are large, growing singly and in small clumps in vitro. By flow cytometry, the immunophenotype was compatible with MCL (i.e. CD5+CD20+CD23-FMC7+). Conventional cytogenetics showed hyperdiploidy with multiple complex karyotypic abnormalities, but no evidence of the t(11;14), proven to be present only by fluorescence in situ hybridization and polymerase chain reaction (PCR) methods. Western blots showed expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein was predominantly phosphorylated. There was expression of tumor suppressor gene products including p53, p16(INK4a), and p21(WAF1). Sequencing of the TP53 gene revealed a mutation (codon 147(valine-->glycine)) in exon 5. Epstein Barr virus was absent. In summary, Mino is a new MCL cell line that may be useful to study the pathogenesis of MCL.
Lai
R
R
Department of Hematopathology, University of Texas, M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Box 72, Houston, TX 77030, USA. raymondlai@mdanderson.org
McDonnell
T J
TJ
O'Connor
S L
SL
Medeiros
L J
LJ
Oudat
R
R
Keating
M
M
Morgan
M B
MB
Curiel
T J
TJ
Ford
R J
RJ
D019943:Amino Acid Substitution; D000782:Aneuploidy; D015703:Antigens, CD / Q000032:analysis; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000032:analysis; D048429:Cell Size; D002869:Chromosome Aberrations; D003062:Codon / Q000235:genetics; D016213:Cyclins / Q000032:analysis; D005091:Exons / Q000235:genetics; D017809:Fatal Outcome; D005260:Female; D016158:Genes, p53; D004854:Herpesvirus 4, Human; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D020522:Lymphoma, Mantle-Cell / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D008875:Middle Aged; D020125:Mutation, Missense; D009363:Neoplasm Proteins / Q000032:analysis; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000473:pathology
D015703:Antigens, CD; D018797:Cell Cycle Proteins; D003062:Codon; D016213:Cyclins; D009363:Neoplasm Proteins