T lymphocytes as targets of gene transfer with Moloney-type retroviral vectors.
Peripheral T lymphocytes are a target of choice for many gene therapeutic strategies. Retrovirus-mediated transduction allows genomic integration and long-term expression of transgenes in target cells. Over many years, low transduction efficiency into primary T lymphocytes has limited clinical application of existing protocols. Recently, gene transfer rates > 50% have been achieved facilitating clinical studies. More attention is thus being focused on the ability of gene-modified cells to carry out innate as well as conferred functions in vivo and the influence of culture conditions, retroviral vector and host response thereon.
University Hospital Eppendorf, Martinistr. 52, 20251 Hamburg, Germany.
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FA
Ayuk
A R
AR
Zander
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B
Fehse
D000818:Animals; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D008979:Moloney murine leukemia virus / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D013601:T-Lymphocytes / Q000502:physiology*; D014161:Transduction, Genetic