Ozone-induced DNA single strand-breaks in guinea pig tracheobronchial epithelial cells in vivo.
Two-month-old male guinea pigs (Dunkin-Hartley strain, specific pathogen free), 4 in each group, were exposed to 0.00, 0.45, and 1.00 ppm O3 for 72 h. The trachea with two main bronchi was removed from each animal after O3 exposure. The trachea lavage fluid was used to measure the protein content as an index of altered tracheobronchial epithelial (TE) cell membrane permeability after O3 exposure. The TE cells were isolated and employed for the determination of DNA single-strand breaks (SSBs) by fluorometric analysis of DNA unwinding (FADU). The statistical significance level was set at alpha =.05. The results show that neither the yield nor the viability of the TE cell from various O3 treatment groups was different from that of controls. Compared to controls, the protein content was elevated significantly after 0.45 ppm O3 exposure; however, the amount of DNA SSBs was not. The number of DNA SSBs increased significantly in the 1.00 ppm O3 exposure group when compared to controls. Regardless of the alkali incubation time at 15 degrees C, the double-stranded DNA left in the alkali TE cell lysate was a linear function of O3 exposure concentrations.
Department of Health and Safety, School of Health and Human Performance, Indiana State University, Terre Haute, Indiana 47809, USA. s-ferng@indstate.edu
Shiaw-Fen
SF
Ferng
D000393:Air Pollutants / Q000493:pharmacokinetics / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001980:Bronchi / Q000187:drug effects* / Q000473:pathology; D002463:Cell Membrane Permeability; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects*; D004847:Epithelial Cells / Q000187:drug effects*; D005434:Flow Cytometry; D006168:Guinea Pigs; D008297:Male; D010126:Ozone / Q000493:pharmacokinetics / Q000633:toxicity*; D014132:Trachea / Q000187:drug effects* / Q000473:pathology
D000393:Air Pollutants; D004277:DNA, Single-Stranded; D010126:Ozone