Purification and partial characterization of a lectin from Canavalia grandiflora benth. seeds.
A D-glucose/D-mannose specific lectin from seeds of Canavalia grandiflora (ConGF) was purified by affinity chromatography on Sephadex G-50. By SDS-PAGE ConGF yielded three protein bands with apparent molecular masses of 29-30 kDa (alpha chain), 16-18 kDa (beta fragment) and 12-13 kDa (gamma fragment), like other related lectins from the genus Canavalia (Leguminosae). ConGF strongly agglutinates rabbit erythrocytes, has a high content of ASP and SER, and its N-terminal sequence (30 residues) is highly similar to the sequences of other related lectins from subtribe Diocleinae.
Depto de Geociências/Universidade Estadual do Ceará, Caixa Postal 6033, CEP 60451-970, Fortaleza-Ceará, Brasil.
V M
VM
Ceccatto
B S
BS
Cavada
E P
EP
Nunes
N A P
NA
Nogueira
M B
MB
Grangeiro
F B M B
FB
Moreno
E H
EH
Teixeira
A H
AH
Sampaio
M A O
MA
Alves
M V
MV
Ramos
J J
JJ
Calvete
T B
TB
Grangeiro
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006241:Haptens / Q000378:metabolism; D006384:Hemagglutination; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D037121:Plant Lectins; D011817:Rabbits; D012639:Seeds / Q000737:chemistry*
D000596:Amino Acids; D006241:Haptens; D037102:Lectins; D037121:Plant Lectins