Flow cytometric determination of lipid peroxidation using fluoresceinated phosphoethanolamine.
In conclusion, we describe the use of fluor-DHPE as a flow cytometric probe to assess lipid peroxidation in erythrocytes. The stability and nonexchangeability of this probe make it suitable for monitoring lipid peroxidation in a particular cell type via flow cytometry, which can be done in the presence of other cells. Application of the probe in erythrocytes from rats fed a vitamin E-deficient diet demonstrated a higher susceptibility to lipid peroxidation among these cells than among erythrocytes from rats fed a normal diet. The current flow cytometric lipid peroxidation detection method can be interfaced directly with several standard techniques that are available to measure specific blood cell populations via flow cytometry.
Maulik
Gautam
G
Department of Adult Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Salgia
Ravi
R
Makrigiorgos
G Mike
GM
D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology; D003287:Contrast Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000378:metabolism; D004983:Ethanolamines / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D019793:Fluorescein / Q000494:pharmacology*; D015227:Lipid Peroxidation; D008856:Microscopy, Fluorescence / Q000379:methods; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species; D013997:Time Factors; D014811:Vitamin E Deficiency
D001555:Benzene Derivatives; D003287:Contrast Media; D004983:Ethanolamines; D017382:Reactive Oxygen Species; C005448:phosphorylethanolamine; C007164:cumene hydroperoxide; D019793:Fluorescein