Urinary level of 1,N(6) -ethenodeoxyadenosine, a marker of oxidative stress, is associated with salt excretion and omega 6-polyunsaturated fatty acid intake in postmenopausal Japanese women.
Excretion of 1,N(6)-ethenodeoxyadenosine (epsilon dA), a marker for lipid peroxidation (LPO)-derived DNA damage was analyzed in urine of nonsmoking postmenopausal women participating in a dietary intervention trial in Northern Japan. Hereby the efficacy of dietary consultation in reducing salt and increasing vitamin C and carotenes during 1 year was estimated. Thirty postmenopausal women, 60-69 years of age, from the intervention group and 30 age-matched women from the control group were randomly selected. The subjects completed a self-administered diet history questionnaire and in the pre- and post-intervention period 48 hr urine and fasting blood samples were collected. epsilon dA in urine was analyzed by an immuno-precipitation-high performance liquid chromatography-fluorescence detection method. epsilon dA excretion (/48 hr) in the 59 postmenopausal Japanese women with complete urine collection ranged from 12-226 pmol at the pre-intervention. At the pre-intervention, epsilon dA excretion was positively associated with urinary salt excretion (R = 0.33, p = 0.01) and omega-6 polyunsaturated fatty acid intake (%energy value, R = 0.28, p = 0.03) in the 59 women. The average epsilon dA excretion in the intervention group was 61 pmol at pre-intervention and 44 pmol at post-intervention (p = 0.14). In the control group, it was 58 pmol at pre-intervention and 75 pmol at post-intervention (p = 0.24). During the intervention period, 18/29 (62%) of the subjects in the intervention group exhibited the decreased excretion and 10/26 (38%) in the control group (p = 0.08). Results from this pilot study suggest urinary epsilon dA as a potential biomarker of DNA damage possibly derived from salt-induced inflammation and LPO; further exploration of epsilon dA in human biomonitoring studies is warranted.
Epidemiology and Biostatistics Division, National Cancer Center Research Institute East, Chiba, Japan. thanaoka@east.ncc.go.jp
Tomoyuki
T
Hanaoka
Jagadeesan
J
Nair
Yoshiko
Y
Takahashi
Satoshi
S
Sasaki
Helmut
H
Bartsch
Shoichiro
S
Tsugane
D000368:Aged; D015415:Biomarkers / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D018592:Cross-Over Studies; D004249:DNA Damage; D003611:Dairy Products; D003839:Deoxyadenosines / Q000652:urine*; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D007163:Immunosorbent Techniques; D007564:Japan; D015227:Lipid Peroxidation; D008875:Middle Aged; D018384:Oxidative Stress; D017698:Postmenopause; D012965:Sodium Chloride / Q000652:urine*; D014815:Vitamins / Q000097:blood
D015415:Biomarkers; D003839:Deoxyadenosines; D004041:Dietary Fats; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D014815:Vitamins; D012965:Sodium Chloride; C032221:1,N(6)-ethenodeoxyadenosine