Expression of alpha and beta Genes of Human Hemoglobin in Escherichia coli.
Total RNA was isolated from human erythrocyte. cDNA fragments coding for alpha and beta genes of human hemoglobins were obtained through RT-PCR and were ligated to plasmid pT7-Blue. Then fragments of alpha and beta genes were cut off and ligated with plasmid pBV220. Sequences identical compared with the published ones were confirmed by DNA sequencing. Bacteria harbouring expression plasmid were induced at 42 degrees, and a band of expressed protein of 16 kD was found in PAGE.
Zhang
Hao
H
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Wang
Quan-Li
QL
Feng
Qi
Q
Du
Zhi-Yan
ZY
Liu
Xiao-Da
XD
Liao
Xing-Rong
XR
Mao
Bing-Zhi
BZ