Evaluation of a sandwich enzyme-linked immunosorbent assay for the measurement of serum heart fatty acid-binding protein.
BACKGROUND
We evaluated the sandwich enzyme-linked immunosorbent assay (ELISA) MARKIT-M for the determination of heart fatty-acid-binding protein (H-FABP).


CONCLUSIONS
The between-run coefficient of variation of this assay was <3.9 and it showed good correlation with a previously established ELISA method. The upper reference limit in 30 healthy individuals was 6.1 microg/L. Admission serum H-FABP was evaluated against myoglobin in 41 patients with suspected myocardial infarction (onset of symptoms < or = 5 h). H-FABP showed the same diagnostic efficiency as myoglobin [area (standard error) under the receiver operating characteristic curve: 0.798 (0.079) for H-FABP, 0.771 (0.085) for myoglobin, P = 0.55]. However, using the upper reference limit as decision cut-off, the sensitivity for H-FABP [91%; 95% confidence interval (CI): 76-98%] was significantly (P = 0.019) higher than that of myoglobin (65%; 95% CI: 47-80%).
Pagani
Franca
F
Laboratorio Analisi Chimico Cliniche 1, Azienda Ospedaliera Spedali Civili, 25125 Brescia, Italy.
Bonora
Roberto
R
Bonetti
Graziella
G
Panteghini
Mauro
M
D015415:Biomarkers; D002352:Carrier Proteins / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D006801:Humans; D009211:Myoglobin / Q000032:analysis; D009363:Neoplasm Proteins; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D025521:Tumor Suppressor Proteins
D015415:Biomarkers; D002352:Carrier Proteins; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009211:Myoglobin; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins