Human heat shock protein 60 (409-424) fragment is recognized by serum antibodies of patients with acute coronary syndromes.
Acute coronary syndromes (ACS), including unstable angina (UA) and acute myocardial infarction (MI), are clinical manifestations of a progressive atherosclerotic process. Antibodies (Ab) to heat shock proteins (hsp) have been reported to be associated with atherosclerosis. Blood samples from 35 patients with ACS and 20 healthy volunteers were tested for Ab to human hsp60 by an enzyme-linked immunosorbent assay (ELISA). Levels of specific serum Ab against hsp60 were significantly elevated in patients with ACS when compared to clinically healthy subjects. To determine the antigenic determinants recognized by these Ab, antibody binding to seven peptides, selected from the hydrophilic and acrophilic regions of the human hsp60 molecule, was assessed. Despite the individual variation in the immune response among patients, one immunodominant region was revealed corresponding to the hsp60 (409-424) peptide. The identification of this epitope may be important for understanding the function of this protein in the atherosclerotic process.
Institute of Maritime and Tropical Medicine, ul. Powstania Styczniowego 9 b St., 81-519 Gdynia, Poland. wysoj@immt.gdynia.pl
Jan
J
Wysocki
Bozena
B
Karawajczyk
Jacek
J
Górski
Artur
A
Korzeniowski
Zbigniew
Z
Maćkiewicz
Gotfryd
G
Kupryszewski
Renata
R
Glośnicka
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000789:Angina, Unstable / Q000209:etiology / Q000276:immunology*; D001161:Arteriosclerosis / Q000209:etiology / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D001426:Bacterial Proteins; D016022:Case-Control Studies; D018834:Chaperonin 60 / Q000235:genetics / Q000276:immunology*; D018833:Chaperonins / Q000276:immunology; D005260:Female; D006801:Humans; D016056:Immunodominant Epitopes / Q000235:genetics; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000276:immunology; D009203:Myocardial Infarction / Q000209:etiology / Q000276:immunology*; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology*
D001323:Autoantibodies; D001426:Bacterial Proteins; D018834:Chaperonin 60; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins