Simple and sensitive method for the determination of chlorpheniramine maleate in human plasma using liquid chromatography-mass spectrometry.
A convenient liquid chromatographic-single quadrupole mass spectrometric (LC-MS) method was developed and validated for the determination of chlorpheniramine maleate (INN name: chlorphenamine) in human plasma. The method had advantages of a single liquid-liquid extraction with diethylether and high sensitivity. The linearity was also excellent over the concentration range of 0.52-20.8 ng/ml of chlorpheniramine maleate. The intra- and inter-day precision and accuracy ranged between 0.0 and 13.9%, showing a good reproducibility. This developed method was successfully applied to analysis of chlorpheniramine maleate in clinical studies.
Pharmacokinetics and Physico-Chemical Property Research Laboratories, Dainippon Pharmaceutical Co., Ltd. 33-94 Enoki-cho, Suita, Osaka 564-0053, Japan. takeshi-takagaki@dainippon-pharm.co.jp
Takeshi
T
Takagaki
Michiaki
M
Matsuda
Yasuyuki
Y
Mizuki
Yoshiaki
Y
Terauchi
D002744:Chlorpheniramine / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods; D006634:Histamine H1 Antagonists / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D006634:Histamine H1 Antagonists; D002744:Chlorpheniramine