Mechanisms of extraribosomal protein biosynthesis in eukaryotes.
The appearance of newly synthesized proteins in the total protein fraction of a cell-free protein-synthesizing system not containing ribosomes and mRNA and enriched with polyenzyme complexes of aminoacyl-tRNA synthetases and corresponding proteins is proved experimentally. For modeling of extraribosomal protein biosynthesis we proposed a molecular polyenzyme mechanism of protein biosynthesis on a protein matrix.
Institute of Physical and Chemical Medicine, Russian Ministry of Health, Moscow.
A V
AV
Mal'tsev
A A
AA
Firsova
E V
EV
Il'ina
V N
VN
Korneev
D000596:Amino Acids / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D000818:Animals; D002474:Cell-Free System; D005057:Eukaryotic Cells / Q000378:metabolism; D007700:Kinetics; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012269:Ribosomal Proteins / Q000096:biosynthesis*
D000596:Amino Acids; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D000604:Amino Acyl-tRNA Synthetases