Gibberellic acid, synthetic auxins, and ethylene differentially modulate alpha-L-Arabinofuranosidase activities in antisense 1-aminocyclopropane-1-carboxylic acid synthase tomato pericarp discs.
Alpha-L-Arabinofuranosidases (alpha-Afs) are plant enzymes capable of releasing terminal arabinofuranosyl residues from cell wall matrix polymers, as well as from different glycoconjugates. Three different alpha-Af isoforms were distinguished by size exclusion chromatography of protein extracts from control tomatoes (Lycopersicon esculentum) and an ethylene synthesis-suppressed (ESS) line expressing an antisense 1-aminocyclopropane-1-carboxylic synthase transgene. alpha-Af I and II are active throughout fruit ontogeny. alpha-Af I is the first Zn-dependent cell wall enzyme isolated from tomato pericarp tissues, thus suggesting the involvement of zinc in fruit cell wall metabolism. This isoform is inhibited by 1,10-phenanthroline, but remains stable in the presence of NaCl and sucrose. alpha-Af II activity accounts for over 80% of the total alpha-Af activity in 10-d-old fruit, but activity drops during ripening. In contrast, alpha-Af III is ethylene dependent and specifically active during ripening. alpha-Af I released monosaccharide arabinose from KOH-soluble polysaccharides from tomato cell walls, whereas alpha-Af II and III acted on Na(2)CO(3)-soluble pectins. Different alpha-Af isoform responses to gibberellic acid, synthetic auxins, and ethylene were followed by using a novel ESS mature-green tomato pericarp disc system. alpha-Af I and II activity increased when gibberellic acid or 2,4-dichlorophenoxyacetic acid was applied, whereas ethylene treatment enhanced only alpha-Af III activity. Results suggest that tomato alpha-Afs are encoded by a gene family under differential hormonal controls, and probably have different in vivo functions. The ESS pericarp explant system allows comprehensive studies involving effects of physiological levels of different growth regulators on gene expression and enzyme activity with negligible wound-induced ethylene production.
Sozzi
Gabriel O
GO
Cátedra de Fruticultura, Departamento de Producción Vegetal, Facultad de Agronomía, Universidad de Buenos Aires, C 1417 DSE Buenos Aires, Argentina.
Greve
L Carl
LC
Prody
Gerry A
GA
Labavitch
John M
JM
D002473:Cell Wall / Q000378:metabolism; D005030:Ethylenes / Q000494:pharmacology; D005638:Fruit / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D005875:Gibberellins / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000187:drug effects / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000737:chemistry / Q000494:pharmacology; D007527:Isoenzymes / Q000187:drug effects / Q000378:metabolism; D008190:Lyases / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D008670:Metals / Q000494:pharmacology; D010858:Pigmentation / Q000187:drug effects; D010937:Plant Growth Regulators / Q000494:pharmacology*; D016372:RNA, Antisense / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D005030:Ethylenes; D005875:Gibberellins; D007210:Indoleacetic Acids; D007527:Isoenzymes; D008670:Metals; D010937:Plant Growth Regulators; D016372:RNA, Antisense; D012965:Sodium Chloride; D013395:Sucrose; C036216:ethylene; C007842:gibberellic acid; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase; D008190:Lyases; C021773:1-aminocyclopropanecarboxylate synthase; D015032:Zinc