A cytochrome c encoded by the nar operon is required for the synthesis of active respiratory nitrate reductase in Thermus thermophilus.
A cytochrome c (NarC) is encoded as the first gene of the operon for nitrate respiration in Thermus thermophilus. NarC is required for anaerobic growth and for the synthesis of active nitrate reductase (NR). The alpha and delta subunits (NarG, NarJ) of the NR were constitutively expressed in narC::kat mutants, but NarG appeared in the soluble fraction instead of associated with the membranes. Our data demonstrate for NarC an essential role in the synthesis of active enzyme and for the attachment to the membrane of the respiratory NR from T. thermophilus.
Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Campus de Cantoblanco, 28049, Madrid, Spain.
Olga
O
Zafra
Sandra
S
Ramírez
Pablo
P
Castán
Renata
R
Moreno
Felipe
F
Cava
Cristina
C
Vallés
Eddy
E
Caro
José
J
Berenguer
D003574:Cytochrome c Group / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009876:Operon / Q000235:genetics; D016963:Thermus thermophilus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
D003574:Cytochrome c Group; D008565:Membrane Proteins; D009097:Multienzyme Complexes; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D009565:Nitrate Reductases; D050901:Nitrate Reductase