GLP-1 and GLP-2 act in concert to inhibit fasted, but not fed, small bowel motility in the rat.
Small bowel motility was studied in rats at increasing (1-20 pmol/kg/min) intravenous doses of either glucagon-like peptide-1 (GLP-1) or glucagon-like peptide-2 (GLP-2) alone, or in combination in the fasted and fed state. There was a dose-dependent inhibitory action of GLP-1 on the migrating myoelectric complex (MMC), where the dose of 5 pmol/kg/min induced an increased MMC cycle length. No effect was seen with GLP-2 alone, but the combination of GLP-1 and GLP-2 induced a more pronounced inhibitory effect, with significant increase of the MMC cycle length from a dose of 2 pmol/kg/min. During fed motility, infusion of GLP-1 resulted in an inhibition of spiking activity compared to control. In contrast, infusion of GLP-2 only numerically increased spiking activity compared to control, while the combination of GLP-1 and GLP-2 resulted in no change compared to control. In summary, this study demonstrates an additive effect of peripheral administration of GLP-1 and GLP-2 on fasted small bowel motility. In the fed state, GLP-1 and GLP-2 seem to display counter-balancing effects on motility of the small intestine.
Department of Physiology, Marmara University, Istanbul, Turkey.
Ayhan
A
Bozkurt
Erik
E
Näslund
Jens Juul
JJ
Holst
Per M
PM
Hellström
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D004347:Drug Interactions; D004435:Eating / Q000502:physiology; D004576:Electromyography; D005215:Fasting / Q000502:physiology*; D005769:Gastrointestinal Motility / Q000187:drug effects*; D005934:Glucagon / Q000097:blood / Q000494:pharmacology*; D052216:Glucagon-Like Peptide 1; D053767:Glucagon-Like Peptide 2; D007275:Injections, Intravenous; D007328:Insulin / Q000097:blood; D007421:Intestine, Small / Q000187:drug effects*; D008297:Male; D010446:Peptide Fragments / Q000097:blood / Q000494:pharmacology*; D010455:Peptides / Q000097:blood / Q000494:pharmacology*; D011498:Protein Precursors / Q000097:blood / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D053767:Glucagon-Like Peptide 2; D007328:Insulin; D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors; D052216:Glucagon-Like Peptide 1; D005934:Glucagon