Engineering Novel Functional Proteins Grafting Active Sites into Natural Scaffolds.
Engineering novel small functional proteins by grafting active sites into small but stable proteins is an efficient protein design method. Combining heterogeneous self-consistent ensemble optimization (hetero-SCEO) with 3D-motif search tool, we developed a system to accomplish such method. It is tested by transferring zinc-binding site of carbonic anhydrase form B to charybdotoxin and its efficiency is demonstrated.
Ye
Yu-Zhen
YZ
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. dingdafu@server.shcnc.ac.cn
Tang
Hai-Xu
HX
Ding
Da-Fu
DF