Chemokine production by natural killer cells from nonagenarians.
In this study we investigated whether purified NK cells, derived from a group of nonagenarian healthy subjects, were able to produce the chemokines MIP-1alpha, RANTES and IL-8, and also characterized the effect of IL-12 or IL-2 immunomodulatory cytokines (that are among the most effective inducers of NK lytic activity and soluble factor secretion) on the induction, in vitro, of these chemokines and on the modulation of the corresponding receptors. This study provides evidence that human NK cells from healthy subjects over 90 years old retain the ability to synthesize MIP-1alpha, Rantes and IL-8 chemotactic cytokines, that NK cells isolated from these subjects can be activated to significantly up-regulate the production of these chemokines in response to stimulation by IL-12 or IL-2 cytokines (even though production remains lower than that observed in young subjects), and that NK cells express the corresponding chemokine receptors.
Laboratorio di Immunologia e Genetica, Istituto di Ricerca Codivilla-Putti, IOR, Bologna, Italy. marianie@alma.unibo.it
Erminia
E
Mariani
Alessandra
A
Meneghetti
Simona
S
Neri
Giovanni
G
Ravaglia
Paola
P
Forti
Luca
L
Cattini
Andrea
A
Facchini
D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000276:immunology*; D018925:Chemokines / Q000096:biosynthesis* / Q000235:genetics; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D018664:Interleukin-12 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D019707:Receptors, Chemokine / Q000032:analysis / Q000235:genetics
D018925:Chemokines; D007376:Interleukin-2; D012333:RNA, Messenger; D019707:Receptors, Chemokine; D018664:Interleukin-12