A Discriminator Base in tDNA(Trp).
The discriminator base G73 was one of the major identity elements of tRNA(Trp). Aminoacylation assay and CD spectrum of tDNA(Trp)-NCCrA demonstrated that under the same pH, the change of discriminator base affected tRNA conformation at the 3' terminus while tDNA(Trp) with the same discriminator base exhibited different conformation and aminoacylation activity under various pH conditions. Our results showed that it was the conformation of tRNA, but not the base itself, that determined the accurate recognition between tRNA and its cognate synthetase.
State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. yxjin@sunm.shcnc.ac.cn
Li
L
Chen
You-Xin
YX
Jin
De-Bao
DB
Wang