Recognition of the carbohydrate modifications to the RgpA protease of Porphyromonas gingivalis by periodontal patient serum IgG.
Periodontal infections by Porphyromonas gingivalis are associated with a sustained systemic IgG antibody response and elevations in local antibody synthesis to this organism. One of the targets of this response is a protease, RgpAcat, which is an important virulence determinant of this organism. Recently, we demonstrated that this molecule is glycosylated and that the glycan chains are immunologically related to P. gingivalis lipopolysaccharide (LPS) (Curtis et al., Infect Immun 1999;62:3816-3823). In the present study, we examined the role of these glycan additions in the immune recognition of RgpAcat, by sera from adult periodontal patients (n = 25). Serum IgG antibody levels to P. gingivalis W50, RgpAcat and LPS and to recombinant RgpA were determined by enzyme-linked immunosorbant assay (ELISA). No correlation was observed between the antibody levels to RgpAcat from P. gingivalis and the recombinant form of this enzyme expressed in Escherichia coli. However, a strong association was found between the recognition of LPS and the wild-type enzyme (R = 0.8926; p = 0.0005). Incorporation of LPS into the ELISA led to a significant reduction (mean 25%; range 0.8-43%, SD = 15; p < 0.05) in the recognition of RgpAcat, but had no effect on the recognition of control antigens. Deglycosylation of RgpAcat led to the abolition of immune recognition by patient serum IgG, which suggests that the glycan additions to this molecule are the principal targets of the immune response. Therefore, glycosylation of the RgpAcat protease may play an important role in immune evasion by shielding the primary structure from immune recognition.
Department of Medical Microbiology, Barts and the London School of Medicine and Dentistry, Queen Mary, University of London, UK.
Jennifer M
JM
Slaney
Minnie
M
Rangarajan
Joseph
J
Aduse-Opoku
Stuart
S
Fawell
Ivan
I
Darby
Denis
D
Kinane
Michael A
MA
Curtis
D018829:Adhesins, Bacterial; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D016525:Antigenic Modulation; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D016866:Bacteroidaceae Infections / Q000097:blood / Q000276:immunology; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007157:Immunologic Surveillance; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000097:blood / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology; D016966:Porphyromonas gingivalis / Q000201:enzymology*
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D011134:Polysaccharides; D003546:Cysteine Endopeptidases