Crystallization and preliminary X-ray crystallographic studies of rice alpha-galactosidase.
alpha-Galactosidases catalyze the hydrolysis of galactooligosaccharides and galactopolysaccharides to alpha-galactose residues and are widely distributed in microorganisms, plants and animals. alpha-Galactosidase from rice (Oryza sativa L. ssp. japonica) was crystallized by the hanging-drop vapour-diffusion method. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 63.1, b = 71.3, c = 85.6 A, and diffract beyond 1.9 A resolution.
Department of Biochemistry, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan.
Zui
Z
Fujimoto
Osamu
O
Kobayashi
Satoshi
S
Kaneko
Mitsuru
M
Momma
Hideyuki
H
Kobayashi
Hiroshi
H
Mizuno
D003460:Crystallization; D018360:Crystallography, X-Ray; D012275:Oryza / Q000201:enzymology*; D000519:alpha-Galactosidase / Q000737:chemistry* / Q000302:isolation & purification
D000519:alpha-Galactosidase