Two-hybrid systems and their usage in infection biology.
Two-hybrid systems are powerful tools to study protein-protein interactions in biological systems. The role of protein-protein interactions involved in pathogenesis of bacterial and viral infections were defined by using yeast or bacterial two-hybrid screens. Examples are given to highlight the specificity of interactions in signaling pathways, in regulation, secretion and structure-function relationships of virulence factors and their cellular targets. Two-hybrid systems were also used to establish large-scale protein interaction maps of viral and bacterial pathogens, that might be useful to identify targets for new drugs or vaccines.
Unité de Biochimie Cellulaire, CNRS URA 2185, Institut Pasteur, Paris, France.
Gouzel
G
Karimova
Daniel
D
Ladant
Agnes
A
Ullmann
D001424:Bacterial Infections / Q000378:metabolism*; D023281:Genomics / Q000379:methods; D006801:Humans; D020798:Two-Hybrid System Techniques; D014777:Virus Diseases / Q000378:metabolism*