Positional cloning of the gene LIMBIN responsible for bovine chondrodysplastic dwarfism.
Chondrodysplastic dwarfism in Japanese brown cattle is an autosomal recessive disorder characterized by short limbs. Previously, we mapped the locus responsible for the disease on the distal end of bovine chromosome 6. Here, we narrowed the critical region to approximately 2 cM by using linkage analysis, constructed a BAC and YAC contig covering this region, and identified a gene, LIMBIN (LBN), that possessed disease-specific mutations in the affected calves. One mutation was a single nucleotide substitution leading to an activation of a cryptic splicing donor site and the other was a one-base deletion resulting in a frameshift mutation. Strong expression of the Lbn gene was observed in limb buds of developing mouse embryos and in proliferating chondrocytes and bone-forming osteoblasts in long bones. These findings indicate that LBN is responsible for bovine chondrodysplastic dwarfism and has a critical role in a skeletal development.
Shirakawa Institute of Animal Genetics, Nishi-shirakawa, Fukushima 961-8061, Japan.
Haruko
H
Takeda
Marika
M
Takami
Tomoko
T
Oguni
Takehito
T
Tsuji
Kazuhiro
K
Yoneda
Hiroaki
H
Sato
Naoya
N
Ihara
Tomohito
T
Itoh
Srinivas R
SR
Kata
Yuji
Y
Mishina
James E
JE
Womack
Yasuo
Y
Moritomo
Yoshikazu
Y
Sugimoto
Tetsuo
T
Kunieda
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004392:Dwarfism / Q000235:genetics / Q000662:veterinary*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010009:Osteochondrodysplasias / Q000235:genetics / Q000662:veterinary*; D010375:Pedigree; D011506:Proteins / Q000235:genetics*
D018076:DNA, Complementary; D011506:Proteins