Determination of myoglobin: comparative evaluation of the new automated VIDAS assay with two other immunoassays.
OBJECTIVE
Myoglobin provides the earliest indication of acute myocardial infarction. In this study, the new myoglobin assay for the VIDAS system (bioMérieux) was evaluated.


METHODS
This assay, using an enzyme-linked fluorescent immunoassay (ELFIA) method, was compared with the Olympus immunoturbidimetric method and with another immunometric method (Immulite turbo) using an enzyme-linked chemiluminescent immunoassay (CLIA).


RESULTS
The CVs for within-and between-run reproducibility are very similar for the tested methods and acceptable linearity ranges were obtained. No significant interference of hemolysis, turbidity and icteria was observed. In the whole cohort, we obtained decreased values over the entire range of the assay with VIDAS and Immulite turbo methods compared to the Olympus assay; this is probably mainly linked to differences in standards used due to the absence of international standardization of the myoglobin determination.


CONCLUSIONS
The new VIDAS myoglobin automated assay provides biologists with a rapid, accurate and reliable determination of myoglobin in plasma samples collected during cardiac workup.
Le Moigne
Françoise
F
Laboratoire de Biochimie, Hôpital cardiologique, avenue de Magellan F-33604 Pessac, France. françoise.lemoigne@chu-bordeaux.fr
Beauvieux
Marie Christine
MC
Derache
Philippe
P
Darmon
Yves Michel
YM
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005260:Female; D005453:Fluorescence; D006801:Humans; D016014:Linear Models; D008163:Luminescent Measurements; D008297:Male; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis; D009211:Myoglobin / Q000097:blood*; D011786:Quality Control; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D013696:Temperature
D009211:Myoglobin