Discovery and analysis of hepatocellular carcinoma genes using cDNA microarrays.
OBJECTIVE
Microarray analysis on a genomic scale was used to profile changes in gene expression accompanying hepatocellular carcinoma.


METHODS
Gene expression profiles of liver tissues from twelve hepatocellular carcinoma samples relative to the gene expression profile of the normal liver tissue were analyzed using 4096 chips and 12800 chips. The results of microarray experiments were verified by the Northern blot technique.


RESULTS
A group of 1,820 genes with altered expression were identified in more than 50% of the patients examined. This highly concordant expression profile included human genes encoding proteins involved in the function of peroxisomes, serum control, polycyclic aromatic hydrocarbon carcinogenesis, cell growth and differentiation, metastasis, the function of the immune system, apoptosis, and remodeling of the cytoskeleton.


CONCLUSIONS
The newly identified genes afford a quantitative view of the changes that accompany liver cancer at the genomic level, enable deeper insights into the molecular basis of disease, and provide an extensive list of potential early-onset molecular markers for improved diagnosis.
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, People's Republic of China. yaoliff@yahoo.com
Yao
Y
Li
Yali
Y
Li
Rong
R
Tang
Hong
H
Xu
Minyan
M
Qiu
Qin
Q
Chen
Juxiang
J
Chen
Zhiren
Z
Fu
Kang
K
Ying
Yi
Y
Xie
Yumin
Y
Mao
D000328:Adult; D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D004798:Enzymes / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods
D004798:Enzymes