Development of a highly sensitive in vitro assay method for biological activity of endotoxin contamination in biological products.
The pyrogen test in rabbits has been replaced by the bacterial endotoxin test. The endotoxin test, however, showed a considerable discrepancy with pyrogenicity and was, therefore, assumed to have an efficacy limitation in directly predicting harmful biological effects of endotoxin. We developed a sensitive in vitro assay method by making use of tumour necrosis factor alpha (TNF-alpha) induction in RAW264.7 cells, which showed a fine correlation with pyrogenicity in rabbits. RAW264.7 cells maintained by serial subculture under an endotoxin-free condition have gained the similar level of sensitivity as the endotoxin test to allow extensive dilutions of a drug for eliminating adverse effects on the cells. The in vitro TNF-alpha induction assay was shown to be capable to detect quantitatively a synergistic effect of a drug and endotoxin. The synergy is assumed necessary to be taken into consideration to define the limit value for the endotoxin test for guaranteeing the similar level of safety as by the pyrogen test.
Department of Safety Research on Biologics, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
Akihiko
A
Yamamoto
Masaki
M
Ochiai
Michiyo
M
Kataoka
Hiromi
H
Toyoizumi
Yoshinobu
Y
Horiuchi
D000818:Animals; D002460:Cell Line; D002624:Chemistry, Clinical / Q000379:methods*; D016207:Cytokines / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000032:analysis* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D016898:Interferon-alpha / Q000378:metabolism; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007700:Kinetics; D008070:Lipopolysaccharides / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D011749:Pyrogens / Q000032:analysis; D011817:Rabbits; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism*
D016207:Cytokines; D004731:Endotoxins; D016898:Interferon-alpha; D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D011749:Pyrogens; D014409:Tumor Necrosis Factor-alpha