Desmoplastic infantile astrocytoma and ganglioglioma: a search for genomic characteristics.
In the present study the clinical data, histology, proliferation rate, DNA ploidy status and the results of TP53 mutation analysis and comparative genomic hybridization (CGH) of three typical cases of desmoplastic infantile astrocytoma and ganglioglioma are presented. Postoperative disease-free intervals of 11, 8 and 3 years were recorded and in none of the cases were radiological signs of tumor recurrence. No TP53 mutations (exons 5-8) were found. CGH analysis revealed loss of 8p22-pter in one case, while in another case gain of 13q21 was detected. In the case with the follow-up of 11 years an aneuploid DNA-flow cytogram along with slightly increased MIB-1 labeling index (LI) was found. The results demonstrate little genetic instability in these low-grade lesions. DNA-aneuploidy seems not to be indicative of tumor progression. It is concluded that the genetic aberrations found in desmoplastic infantile ganglioglioma differ from those encountered in common astrocytomas.
Department of Pathology, University Hospital Rotterdam-Dijkzigt, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands. kros@path.fgg.eur.nl
Johan M
JM
Kros
Ernst J
EJ
Delwel
T H Rob
TH
de Jong
Herve L J
HL
Tanghe
Peter R W A
PR
van Run
Kees
K
Vissers
Janneke C
JC
Alers
D034961:Antigens, Nuclear; D001254:Astrocytoma / Q000235:genetics* / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D018303:Ganglioglioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007223:Infant; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D009687:Nuclear Proteins / Q000032:analysis; D011003:Ploidies; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D034961:Antigens, Nuclear; D004273:DNA, Neoplasm; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D016159:Tumor Suppressor Protein p53