tej defines a role for poly(ADP-ribosyl)ation in establishing period length of the arabidopsis circadian oscillator.
In a genetic screen for altered circadian period length in Arabidopsis, we isolated a mutant with a long free-running period. The tej mutation acts independently of light quality and quantity. It affects clock-controlled transcription of genes in Arabidopsis and alters the timing of the photoperiod-dependent transition from vegetative growth to flowering. Map-based cloning of TEJ identified a poly(ADP-ribose) glycohydrolase (PARG). An inhibitor of poly(ADP-ribosyl)ation rescued the period phenotype of tej mutant and shortened the period length of wild-type plants. Posttranslational poly(ADP-ribosyl)ation of an oscillator component may contribute to setting the period length of the Arabidopsis central oscillator.
Panda
Satchidananda
S
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Poirier
Guy G
GG
Kay
Steve A
SA
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D001549:Benzamides / Q000494:pharmacology; D001683:Biological Clocks / Q000235:genetics*; D002940:Circadian Rhythm / Q000235:genetics*; D003001:Cloning, Molecular; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010507:Periodicity; D010641:Phenotype; D010775:Photic Stimulation; D017440:Photoperiod; D011064:Poly Adenosine Diphosphate Ribose / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D029681:Arabidopsis Proteins; D001549:Benzamides; D004791:Enzyme Inhibitors; D011064:Poly Adenosine Diphosphate Ribose; C025160:3-aminobenzamide; D006026:Glycoside Hydrolases; C022407:poly ADP-ribose glycohydrolase