Glutamatergic modulation of GABAergic signaling among hippocampal interneurons: novel mechanisms regulating hippocampal excitability.
OBJECTIVE
Because interneurons play a central role in regulating the excitability of the hippocampal formation, it is important to understand the mechanisms that modulate gamma-aminobutyric acid (GABA)ergic signaling among them. This study addresses the modulation of GABA release from interneuron terminals by presynaptic glutamate receptors.


METHODS
Whole-cell recordings were obtained from CA1 stratum radiatum interneurons in guinea pig hippocampal slices. Selective agonists and blockers of glutamate receptors were used to study modulation of GABAergic transmission by group III metabotropic receptors or kainate receptors. Antidromic action-potential initiation also was analyzed by stimulating the axons of interneurons.


RESULTS
Agonists of group III metabotropic glutamate receptors attenuated monosynaptic GABAergic signals in interneurons, but not in pyramidal neurons, in agreement with anatomic evidence on the distribution of these receptors. Submicromolar kainate enhanced the frequency and amplitude of spontaneous GABAergic signals in interneurons. Kainate also depolarized the axons of hippocampal interneurons, and triggered spontaneous ectopic action potentials in axons. Synaptically released glutamate reproduced many of the effects of both agonists, implying that these receptors can sense the ambient glutamate concentration, and therefore indirectly respond to the excitatory traffic in the hippocampus. When the two classes of receptors were stimulated simultaneously, complex interactions were obtained.


CONCLUSIONS
Group III metabotropic receptors and kainate receptors profoundly affect GABAergic signaling among interneurons of the hippocampus. Glutamatergic modulation of GABAergic signaling among interneurons represents a novel class of mechanisms that potentially plays a major role in determining the initiation, propagation, and termination of seizures.
Institute of Neurology, UCL Queen Square, London, WC1N 3BG, UK. d.kullmann@ion.ucl.ac.uk
Dimitri M
DM
Kullmann
Alexey
A
Semyanov
D000818:Animals; D001369:Axons / Q000502:physiology; D004594:Electrophysiology; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D018756:GABA Antagonists / Q000378:metabolism; D018698:Glutamic Acid / Q000502:physiology*; D006168:Guinea Pigs; D006624:Hippocampus / Q000502:physiology*; D066298:In Vitro Techniques; D007395:Interneurons / Q000502:physiology*; D009433:Neural Inhibition / Q000502:physiology; D018092:Kainic Acid Receptors / Q000502:physiology; D018094:Receptors, Metabotropic Glutamate / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D018756:GABA Antagonists; D018698:Glutamic Acid; D018092:Kainic Acid Receptors; D018094:Receptors, Metabotropic Glutamate; D005680:gamma-Aminobutyric Acid; C102718:metabotropic glutamate receptor 3