Identification of the novel allele B*4427 and a confirmatory sequence (B*44022).
This report presents a novel allele, HLA-B*4427, which was identified in a bone marrow donor of Caucasian origin, and a confirmatory sequence (B*44022). Sequence analysis revealed the new allele differs from B*44021 by a single nucleotide exchange at position 668 (C-->T), which is located in exon 4. At the protein level, it is the only B*44 variant to produce an Ala in place of a Val at codon 199. Its structure suggests that it may have originated from a point mutation in B*44021 or by gene conversion with a variety of HLA-B alleles. Cloning and sequencing of the allele B*44022 revealed a sequence identical to B*44021 and B*44 exon 4, with the codon GTC (Val) in position 199.
CNR Institute of Tissue Typing and Dialysis Regional Center of Immunohematology and Tissue Typing, Department of Experimental Medicine, University of L'Aquila, Italy.
A
A
Canossi
F
F
Papola
G
G
Liberatore
T
T
Del Beato
D
D
Piancatelli
A
A
Tessitore
M T
MT
Vicentini
D
D
Maccarone
A
A
Aureli
C
C
Cervelli
M
M
Di Rocco
C U
CU
Casciani
D
D
Adorno
D000483:Alleles; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D015235:HLA-B Antigens / Q000235:genetics*; D059948:HLA-B44 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
D015235:HLA-B Antigens; C471439:HLA-B*44:27 antigen; D059948:HLA-B44 Antigen