Plasma extravasation induced by dietary supplemented histamine in histamine-free mice.
Histidine decarboxylase (HDC) synthesizes endogenous histamine from histidine in mammals. To evaluate the role of histamine in skin allergic reaction, we used HDC gene knockout mice lacking histamine. No plasma extravasation reaction was observed in HDC-/- mice after passive cutaneous anaphylaxis (PCA) test. Compound 48/80, a mast cell granule depletor, produced plasma extravasation inHDC+/+ mice but no extravasation in HDC-/- mice. Interestingly, orally administered histamine was distributed in the skin in HDC-/- mice and in these histamine-supplemented mice the plasma extravasation reaction was observed after the injection of compound 48/80 and the PCA test. Cultured bone marrow-derived mast cells of HDC-/- mice took up histamine from the histamine-supplemented medium into the secretory granules. The absorbed histamine was released in response to the same antigen and antibody combination used as in PCA test. In contrast to the immediate-type response, the delayed-type hypersensitive response, observed as a thickening of the ear skin after trinitrochlorobenzene challenge (following sensitization), showed no differences between HDC+/+ and HDC-/- mice. Therefore, among the allergic skin reactions, histamine is revealed to be an important mediator especially for the plasma extravasation in an immediate-type allergy model.
Ohtsu
Hiroshi
H
Department of Pharmacology, Tohoku University Graduate School of Medicine, Sendai, Japan. ohtsua@mail.cc.tohoku.ac.jp
Kuramasu
Atsuo
A
Tanaka
Satoshi
S
Terui
Tadashi
T
Hirasawa
Noriyasu
N
Hara
Masahiro
M
Makabe-Kobayashi
Yoko
Y
Yamada
Noboru
N
Yanai
Kazuhiko
K
Sakurai
Eiko
E
Okada
Mikiko
M
Ohuchi
Kazuo
K
Ichikawa
Atsushi
A
Nagy
Andras
A
Watanabe
Takehiko
T
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D002199:Capillary Permeability; D019587:Dietary Supplements; D004487:Edema / Q000209:etiology*; D006632:Histamine / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000502:physiology*; D006636:Histamine Release; D006640:Histidine Decarboxylase / Q000172:deficiency; D006969:Hypersensitivity, Immediate / Q000209:etiology; D007408:Intestinal Absorption; D008407:Mast Cells / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000502:physiology; D026901:Membrane Transport Proteins; D051379:Mice; D018345:Mice, Knockout; D009479:Neuropeptides; D010323:Passive Cutaneous Anaphylaxis; D050492:Vesicular Biogenic Amine Transport Proteins
D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009479:Neuropeptides; D050492:Vesicular Biogenic Amine Transport Proteins; D006632:Histamine; D006640:Histidine Decarboxylase