Improved energetic recovery of skeletal muscle in response to ischemia and reperfusion injury followed by in vivo 31P-magnetic resonance spectroscopy.
It is of great clinical interest to improve postischemic tissue recovery during microsurgical transfers. The effect of singlet oxygen energy (SOE) as photon illumination at 634 nm on rat skeletal muscle during ischemia and postischemic reperfusion was investigated noninvasively and continuously by in vivo (31)P-magnetic resonance spectroscopy ((31)P-MRS). A model of pedicled rat rectus femoris muscle was used, where phosphorous metabolites were followed before onset of ischemia (control), after 4 h of ischemia, and after 1 h of reperfusion. Two groups were studied: one control group (n = 10), and one SOE-treated group (n = 10). Blood perfusion was measured by laser Doppler flowmetry (LDF) during the study. After 4 h of ischemia, ATP levels were 72% and 51% of normal control values in the illuminated group and the control group, respectively (P < 0.05). After 1 h of postischemic reperfusion, phosphocreatine (PCr) recovered to 79% and adenosine triphosphate (ATP) to 71% in the illuminated group, whereas in the control group, the recovery was 57% and 51%, respectively (P < 0.05). It is concluded that singlet oxygen energy has a beneficial effect on the energy state of skeletal muscle during ischemia and postischemic reperfusion.
Bioenergetics Group, Department of Surgery, Wallenberg Laboratory, Sahlgrenska University Hospital, Göteborg University, 413 45 Göteborg, Sweden.
Jonas
J
Lundberg
Ann
A
Lindgård
Anna
A
Elander
Bassam
B
Soussi
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004735:Energy Transfer; D007511:Ischemia / Q000378:metabolism; D008029:Lighting; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply*; D010100:Oxygen; D010759:Phosphorus Isotopes; D017785:Photons; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology
D010759:Phosphorus Isotopes; D000255:Adenosine Triphosphate; D010100:Oxygen