Mammary secretion of oestrogens in the cow.
Two experiments in vivo and one experiment in vitro were conduced to examine the mechanisms involved, which lead to mammary secretion of oestrogens and its importance for milk production and udder health in cows. In experiment 1 in six cows of the White-Black breed on day 268 of pregnancy catheters were inserted into uterine vein of pregnant horn, the abdominal aorta and the caudal superficial epigastric (milk) vein. Blood samples for estimation of oestrone, oestrone sulphate, oestradiol-17alpha and -17beta by RIA were obtained daily from day 7 pre-partum until day 1 post-partum. Only the concentration of oestradiol-17beta was statistically higher (P< or =0.01) in mammary venous plasma than in aortal and uterine plasma. In experiment 2 forty late-pregnant cows were divided into two groups according to their milk production in the previous lactation: group 1 (n=20) high-yielding cows (>6500kg milk per lactation), and group 2 (n=20) low-yielding cows (<3700kg milk per lactation). Blood samples for measurement of oestradiol-17beta by RIA were collected from milk and tail veins every fourth day during a period from day 20 prior to parturition to day 4 post-partum. The concentration of oestradiol-17beta was significantly higher (P< or =0.01) in the milk vein than in the peripheral plasma from day 12 pre-partum to parturition. In high-yielding cows the level of oestradiol-17beta in mammary venous blood was significantly higher (P< or =0.01) than in low-yielding cows. In six cows with pathological udder oedema ante-partum the concentration of oestradiol-17beta in milk vein was significantly higher (P< or =0.05) than in control cows. There were no statistically significant differences in the levels of oestradiol-17beta in cows with clinical mastitis (n=10) during 2 weeks after parturition and without it (P> or =0.05). In an in vitro experiment, homogenates of mammary tissue collected on day 7 pre-partum from two cows were incubated with 3H-androstendione. After incubation the samples were extracted and 3H-oestradiol-17beta was separated by HPLC. 3H-oestradiol-17beta was formed in a total yield of 37%. These results indicate that oestrone, oestrone sulphate and oestradiol-17alpha are not secreted by bovine mammary gland. Furthermore, the secretion of oestradiol-17beta starts about day 12 pre-partum and is associated with milk yield and udder oedema. Preliminary in vitro study suggests the synthesis of oestradiol-17beta by mammary tissue.
Department of Obstetrics and Pathology of Reproduction, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland. jantom@moskit.uwm.edu.pl
T
T
Janowski
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S
Zduńczyk
J
J
Malecki-Tepicht
W
W
Barański
A
A
Raś
D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D001012:Aorta, Abdominal; D002417:Cattle / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D004958:Estradiol / Q000032:analysis / Q000097:blood / Q000378:metabolism; D004967:Estrogens / Q000032:analysis / Q000378:metabolism*; D004970:Estrone / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D008321:Mammary Glands, Animal / Q000098:blood supply / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000378:metabolism; D008892:Milk / Q000737:chemistry; D011247:Pregnancy; D014316:Tritium; D014599:Uterus / Q000098:blood supply; D014680:Veins
D004967:Estrogens; D014316:Tritium; D004970:Estrone; D000735:Androstenedione; D004958:Estradiol; C017296:estrone sulfate