Carrageenan-induced inclusions in mammary myoepithelial cells.
The purpose of this investigation was to characterize the ultrastructural changes that occur in mammary myoepithelial cells (MMEC) following exposure in tissue culture to low concentrations of lambda-carrageenan, a sulfated polysaccharide commonly used as a food additive. MMEC were obtained from reduction mammoplasty, grown in tissue culture, exposed for varying durations to low concentrations (0.0014%-0.0001%) of lambda-carrageenan, and examined by transmission electron microscopy, following staining for acid phosphatase and for aryl sulfatase. Carrageenan appeared to enter the cells by membrane-associated endocytic vesicles and accumulate in endosomes and lysosomes. Unusual lamellar inclusions were identified within lysosomes of the MMEC, and lysosomal vacuolation arose in association with the inclusions. The observed changes appeared to lead to destruction of the MMEC by release of proteolytic enzymes from the distorted lysosomes, similar to the process observed in lysosomal storage diseases.
Department of Internal Medicine, University of Iowa, Iowa City 52242-1081, USA.
J K
JK
Tobacman
K S
KS
Walters
D000135:Acid Phosphatase / Q000378:metabolism; D001940:Breast / Q000187:drug effects* / Q000648:ultrastructure; D002351:Carrageenan / Q000633:toxicity*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects* / Q000648:ultrastructure; D005260:Female; D005503:Food Additives / Q000633:toxicity*; D006801:Humans; D002479:Inclusion Bodies / Q000648:ultrastructure*; D016462:Mammaplasty
D005503:Food Additives; D002351:Carrageenan; D000135:Acid Phosphatase