Construction of Biotinylated Annexin V for Detection of Apoptosis.
The biotinylated annexin V was generated by genetic engineering method. The encoding region of annexin V was fused with a gene coding for the carboxyl terminal 87 residues of Escherichia coli biotin carboxyl carrier protein. The fused gene was expressed in E.coli and the annexin V was biotinylated in vivo. The biotinylation efficiency was about 60% as detected by HPLC. The biotinylated annexin V was purified by avidin affinity chromatography and used to detect the apoptosis of the neurons induced by morphine.
Shanghai Institute of Biochemistry the Chinese Academy of Sciences Shanghai 200031 China.
Jian-Xin
JX
Sun
Zhe-Yu
ZY
Chen
Zhen-Dong
ZD
You
Xiang-FU
XF
Wu