Effects of UV irradiation on selected pathogens in peptone water and on stainless steel and chicken meat.
Effects of intensity and processing time of 254 nm UV irradiation on Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella Typhimurium were investigated. Intensities measured at 5.08, 10.1, 15.2, and 20.3 cm from the light source were 1.000, 500, 250, and 150 microW/cm2, respectively. Intensities of 250 or 500 microW/cm2 reduced all suspended pathogen cells in peptone water about 5 log cycles after 2 min and completely inactivated L. monocytogenes and E. coli O157:H7 after 3 min by reductions of 8.39 and 8.64 log cycles, respectively. Intensities of 250 or 500 microW/cm2 also reduced (P < or = 0.05) the tested pathogens inoculated on stainless steel (SS) chips, and E. coli O157:H7 was completely destroyed at 500 microW/cm2 for 3 min. After UV treatment for 3 min at 500 microW/cm2, all selected pathogens on chicken meat with or without skin showed reduction ranges from 0.36 to 1.28 log cycles. Results demonstrated that UV irradiation could effectively decrease pathogens in peptone water and on SS but that it was less effective on chicken meat.
Department of Food Science and Technology, Mississippi State University, Mississippi State 39762, USA.
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Kim
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Silva
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Chen
D000818:Animals; D002645:Chickens; D004307:Dose-Response Relationship, Radiation; D019453:Escherichia coli O157 / Q000254:growth & development / Q000528:radiation effects*; D005514:Food Irradiation / Q000379:methods*; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000254:growth & development / Q000528:radiation effects*; D008460:Meat / Q000382:microbiology*; D010461:Peptones; D012486:Salmonella typhimurium / Q000254:growth & development / Q000528:radiation effects*; D012867:Skin / Q000382:microbiology / Q000528:radiation effects; D013193:Stainless Steel / Q000528:radiation effects; D014466:Ultraviolet Rays; D014867:Water; D014871:Water Microbiology
D010461:Peptones; D014867:Water; D013193:Stainless Steel