A nucleic acid sequence-based amplification method to detect Salmonella enterica serotype enteritidis strain PT4 in liquid whole egg.
Nucleic acid sequence-based amplification (NASBA) was applied to the detection of Salmonella enterica cells in liquid whole egg. Samples (25 g) of liquid whole egg inoculated with Salmonella cells were enriched for 16 h in buffered peptone water, and the NASBA procedure was effective in detecting the presence of Salmonella in samples inoculated with 10 to 100 CFU prior to enrichment.
Cook
N
N
Central Science Laboratory, Sand Hutton, York, UK. n.cook@csl.gov.uk
Ellison
J
J
Kurdziel
A S
AS
Simpkins
S
S
Hays
J P
JP
D000818:Animals; D015169:Colony Count, Microbial; D004531:Eggs / Q000382:microbiology*; D005516:Food Microbiology; D021141:Nucleic Acid Amplification Techniques; D012329:RNA, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012477:Salmonella enteritidis / Q000235:genetics*; D021161:Self-Sustained Sequence Replication / Q000379:methods*; D017423:Sequence Analysis, RNA
D012329:RNA, Bacterial