New exopolysaccharides produced by Aureobasidium pullulans grown on glucosamine.
The polysaccharides produced by Aureobasidium pullulans, grown using glucosamine as the carbon source, were investigated by means of methylation analysis, affinity chromatography and NMR spectroscopy. The results indicated that, besides a small amount of pullulan, this micro-organism was capable of producing-in low yields-mixtures of at least two different complex polysaccharides containing mainly mannose and galactose. (1)H NMR spectra of two fractions obtained by lectin affinity chromatography indicated that one polymer was constituted exclusively of mannose residues while the other contained both galactofuranosyl and mannopyranosyl residues.
Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, via L. Giorgieri 1, I-34127, Trieste, Italy.
Paola
P
Cescutti
Raffaella
R
Pupulin
Franco
F
Delben
Maria
M
Abbate
Mariella
M
Dentini
Lorenzo
L
Sparapano
Roberto
R
Rizzo
Vittorio
V
Crescenzi
D003208:Concanavalin A / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D005944:Glucosamine / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D003904:Mitosporic Fungi / Q000254:growth & development / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis*
D003470:Culture Media; D011134:Polysaccharides; D003208:Concanavalin A; D005944:Glucosamine