Effect of combined photodynamic and chemotherapeutic treatment on lymphoma cells in vitro.
We investigated the cytotoxic and apoptotic effects of a combination of photodynamic therapy (PDT) and cisplatin (CDDP) on L5178Y (LY) cells. Treatment with PDT by photofrin((R)) (5 microg/ml) alone or with CDDP (20 microg/ml) alone killed 41.5+/-8.5% or 42.9+/-6.5% of LY cells, respectively, while a combination of these two treatments killed 99.7+/-0.6%. Apoptotic cell death after combination treatment was also revealed to be 49.6+/-7.8% compared to 12.4+/-3.4% after PDT alone, and 18.8+/-2.6% after CDDP. This study demonstrated that combined treatment of PDT and CDDP results in enhanced apoptotic cell death as well as a cytotoxic effect on LY cells.
Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan. d400018r@stcc.nagasaki-u.ac.jp
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Nonaka
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Ikeda
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Inokuchi
D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D002945:Cisplatin / Q000633:toxicity*; D017323:Dihematoporphyrin Ether / Q000633:toxicity*; D006801:Humans; D010778:Photochemotherapy; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D017323:Dihematoporphyrin Ether; D002945:Cisplatin