Differential susceptibility of photosynthesis to light-chilling stress in rice (Oryza sativa l.) depends on the capacity for photochemical dissipation of light.
The primary target for light-chilling stress in chilling-sensitive cucumber leaves is the chloroplast Cu,Zn-Superoxide dismutase, followed by subsequent inactivation of the photosystem (PS) I by reactive oxygen species (ROS). To test this hypothesis, two rice cultivars that were different in their ecological origins (a chilling-resistant Stejaree 45 and a chilling-sensitive Milyang 23) were evaluated with respect to photosynthetic properties, the ROS scavenging system, and expression of genes that are involved in sucrose synthesis and allocation upon the light-chilling stress. As expected, when the leaves were exposed to various low temperatures with illumination (150 micromol m(-2)s(-1)) for 6 h, the leaf photosynthesis of Milyang 23 decreased faster than that of Stejaree 45. The light-chilling induced differential photoinhibition of photosynthesis between the two cultivars was caused by the photoin-activation of PSII but not of PSI, since the potential quantum yield of PSII followed a similar trend to the changes in photosynthetic rates. The activities of the two chloroplastic antioxidant enzymes (superoxide dismutase and ascorbate peroxidase) that are known to be sensitive to oxidative stress were barely affected by the light-chilling treatments. Among various genes in sucrose metabolism (such as cytosolic FBPase, SPS, SUT, SuSy, and AGPase), the transcript levels of SuSy in Milyang 23 were significantly decreased by light-chilling stress compared to that of Stejaree 45. Based on these results, we propose that PSII, not PSI, is the sensitive site for light-chilling stress in chilling-sensitive rice. The extent of PSII photoinhibition depends on its capacity for the photochemical utilization of light.
Jeong
Suk Weon
SW
Department of Biology, Chungnam National University, Daejeon, Korea.
Choi
Sun Mi
SM
Lee
Dong Sook
DS
Ahn
Sang Nag
SN
Hur
Yoonkang
Y
Chow
Wah Soon
WS
Park
Youn-Il
YI
D000064:Acclimatization; D003080:Cold Temperature; D008027:Light; D012275:Oryza / Q000737:chemistry / Q000201:enzymology / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism; D010777:Photochemistry; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology / Q000502:physiology; D015222:Sodium Channels / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D045322:Photosynthetic Reaction Center Complex Proteins; D015222:Sodium Channels; D013395:Sucrose; D013482:Superoxide Dismutase; D010100:Oxygen