Purification and Characterization of a Novel Anticoagulation Factor (ACF II) from the Venom of Agkistrodon acutus.
A novel anticoagulation factor (ACF II) was first isolated from the venom of Agkistrodon acutus by DEAE-Sephadex A-50, Sephadex G-75 and CM-Sephadex C-50 column chromatography. The purified ACF II showed a single band in PAGE, S DS-PAGE and IEF-PAGE, respectively. ACF II consisted of two peptide chains lin ke d together by disulfide bond(s) and the molecular weights of the two peptide cha ins were about 14.6 kD as determined by SDS-PAGE. The isoelectric point of ACF II was 7.0. ACF II possessed distinct anticoagulant activity and its minimum conc entration to prolong plasma prothrombin time in vitro was 0.4 mg/L. No throm bin- like activity, fibrinolytic activity, phospholipase A(2) activity, haemorrhagic activity, o r lethal activity was detected in ACF II, which may be useful as an effective ve nom-based anticoagulant.
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China. qliu@ustc.edu.cn
Xiao-Long
XL
Xu
Qing-Liang
QL
Liu