Evaluation of antilipid peroxidative action of propolis ethanol extract.
The antilipid peroxidative action of the ethanol extract of Brazilian propolis at a concentration of 47% (w/v) was evaluated by examining the inhibitory effect of the extract on the formation of hydroperoxide- and endoperoxide-type lipid peroxides during heating of authentic polyunsaturated fatty acids and on Fe(3+)-ADP/ascorbic acid- and Fe(3+)-ADP/NADPH-dependent lipid peroxidation reactions in rat liver microsomes. Hydroperoxide-type lipid peroxides were measured by the haemoglobin-methylene blue method and endoperoxide-type lipid peroxides by the thiobarbituric acid (TBA), Fe(3+)-TBA and LPO-586 methods. Propolis ethanol extract inhibited dose-dependently the formation of hydroperoxide- and endoperoxide-type lipid peroxides during heating of linoleic acid, linolenic acid or arachidonic acid and the amount of the extract causing a half inhibition of these lipid peroxide formations ranged between 20 and 75 microg. Propolis ethanol extract inhibited dose-dependently both Fe(3+)-ADP/ascorbic acid- and Fe(3+)-ADP/NADPH-dependent lipid peroxidation reactions in rat liver microsomes when lipid peroxides produced in both reactions were measured by the TBA method. The amount of propolis extract causing a half inhibition of the Fe(3+)-ADP/ascorbic acid-dependent lipid peroxidation was about 5 microg, while that of the extract causing a half inhibition of the Fe(3+)-ADP/NADPH-dependent lipid peroxidation was about 0.15 microg. These results indicate that the propolis ethanol extract exerts an antilipid peroxidative action at very low doses.
Department of Biochemistry, School of Health Sciences, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
Rikio
R
Shinohara
Yoshiji
Y
Ohta
Takahiro
T
Hayashi
Takeyuki
T
Ikeno
D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D007700:Kinetics; D019787:Linoleic Acid / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D011429:Propolis / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017962:alpha-Linolenic Acid / Q000378:metabolism
D016166:Free Radical Scavengers; D017962:alpha-Linolenic Acid; D016718:Arachidonic Acid; D000431:Ethanol; D000244:Adenosine Diphosphate; D011429:Propolis; D019787:Linoleic Acid; D001205:Ascorbic Acid