Tunica albuginea acellular matrix graft for penile reconstruction in the rabbit: a model for treating Peyronie's disease.
OBJECTIVE
To evaluate the use of an acellular matrix graft of the tunica albuginea for functional penile reconstruction in severe cases of Peyronie's disease.


METHODS
In 18 rabbits, an acellular matrix graft of the tunica albuginea was used to cover a 4 x 8 mm tunical defect, and six animals each were killed 1, 3 and 6 months later; four unoperated animals served as histological controls. Before death an erection was induced by papaverine, with the quality classified on a scale of 0-5, and cavernosography performed. After death the penis was prepared for histological study, and the cell number, collagen and elastic fibre content evaluated in the regenerated matrix, and in control specimens and four unimplanted matrices.


RESULTS
Of 18 experimental animals, 11 had normal erections before death, four had slight penile deviation and three developed no erection. Failure was caused by severe postoperative haematoma, resulting in scar tissue. There was no graft rejection. Histologically there was no difference between natural and regenerated tunica. The collagen content and cell number were not significantly different in regenerated and control samples. There were significantly fewer elastic fibres in the unimplanted grafts and the 1-month group, but in later samples this difference was no longer evident.


CONCLUSIONS
The homologous acellular matrix graft of the tunica albuginea warrants further evaluation as an alternative treatment in Peyronie's disease, despite some postoperative failures. The advantage of this orthotopic biomaterial is its rapid integration, with no rejection.
Wefer
J
J
Department of Urology, University of California School of Medicine, San Francisco, California 94143-0738, USA.
Schlote
N
N
Sekido
N
N
Sievert
K-D
KD
Wefer
A E
AE
Nunes
L
L
Bakircioglu
M E
ME
Dahiya
R
R
Tanagho
E A
EA
D000818:Animals; D008297:Male; D023421:Models, Animal; D010411:Penile Induration / Q000601:surgery*; D010413:Penis / Q000601:surgery*; D011817:Rabbits; D019651:Plastic Surgery Procedures / Q000379:methods; D013524:Surgical Flaps