[Role of vascular endothelial growth factor overexpression in ovarian tumor invasion and mechanism].
OBJECTIVE
To study the role of vascular endothelial growth factor (VEGF) overexpression in ovarian tumor metastasis and associated mechanism.


METHODS
The VEGF cDNA was transfected into ovarian tumor cell lines CAOV3 and COC1. Transfected and nontransfected cells were screened for VEGF, gelatinase A (MMP-2) mRNA and protein by reverse transcription polymerase chain reaction (RT-PCR), Western blot and substrate zymography, respectively. The invasive ability of two ovarian tumor cell lines was analysed with Boyden chamber invasion assay before and after VEGF cDNA transfection.


RESULTS
The expression of VEGF, MMP-2 mRNA and protein were increased (P < 0.05) after VEGF cDNA transfection, detected by RT-PCR, Western blot and substrate zymography. After VEGF cDNA transfection, the mean invasion percentage of two ovarian tumor cells [CAOV3: (42.5 +/- 4.1)%; COC1: (26.8 +/- 2.4)%] were higher than that before transfection [CAOV3: (24.7 +/- 1.9)%; COC1: (8.6 +/- 1.1)%, P < 0.05].


CONCLUSIONS
The expression of VEGF correlates to the in vitro invasion of ovarian tumor cell and induction of MMP-2 by VEGF is a key component of VEGF-induced ovarian tumor cell invasion.
Lu
Yunping
Y
Department of Ostetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030, China.
Zhang
Ali
A
Wang
Shixuan
S
Li
Jin
J
Wang
Changyu
C
Ma
Ding
D
D018076:DNA, Complementary / Q000235:genetics; D016228:Endothelial Growth Factors / Q000235:genetics / Q000502:physiology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008222:Lymphokines / Q000235:genetics / Q000502:physiology*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D018076:DNA, Complementary; D016228:Endothelial Growth Factors; D008222:Lymphokines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D020778:Matrix Metalloproteinase 2