Minimally disturbed, multicycle, and reproducible synchrony using a eukaryotic "baby machine".
A eukaryotic "baby machine" has been developed that produces synchronized cultures that display up to four synchronous cell cycles. That such cells can be produced implies that methods unable to produce successive synchronized cell cycles may not actually synchronize cells. But most important, the baby machine method now opens the way for the study of the cell cycle of minimally disturbed, artifact-free, well-synchronized, mammalian cells.
Cooper
Stephen
S
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA. cooper@umich.edu
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division; D002478:Cells, Cultured / Q000166:cytology*; D005057:Eukaryotic Cells / Q000166:cytology*; D005434:Flow Cytometry; D007700:Kinetics; D051379:Mice; D013997:Time Factors; D014407:Tumor Cells, Cultured