gamma-aminobutyric acid transporter-mediated current from bipolar cells in tiger salamander retinal slices.
About 10% of bipolar cells in salamander retina synthesize and take up gamma-aminobutyric acid (GABA), and may use GABA as a neurotransmitter. As GABA uptake is electrogenic, bipolar cells expressing GABA transporters (GATs) should give transport current (IGAT) to extracellular GABA. Using whole-cell patch recording, 28 bipolar cells responded to 30-200 microM GABA puffed to the axon terminals with a picrotoxin (PTX)-sensitive chloride current (ICI) only. Another three bipolar cells had, in addition to ICI, a PTX-resistant, sodium-dependent current that was completely and reversibly blocked by NO-711, an IGAT inhibitor, indicating that this component was an IGAT. This finding provides further support for a subset of GABAergic bipolar cells in the salamander retina.
Department of Neurobiology and Behavior, State University of New York at Stony Brook, Stony Brook, NY 11794, USA. cyang@neurobio.sunysb.edu
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D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D018756:GABA Antagonists / Q000494:pharmacology; D050485:GABA Plasma Membrane Transport Proteins; D007473:Ion Channels / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D026901:Membrane Transport Proteins; D009557:Nipecotic Acids / Q000494:pharmacology; D027361:Organic Anion Transporters; D010091:Oximes / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D010852:Picrotoxin / Q000494:pharmacology; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D014562:Caudata / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000494:pharmacology
D002352:Carrier Proteins; D018756:GABA Antagonists; D050485:GABA Plasma Membrane Transport Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009557:Nipecotic Acids; D027361:Organic Anion Transporters; D010091:Oximes; D010852:Picrotoxin; D012964:Sodium; D005680:gamma-Aminobutyric Acid; C078793:NNC 711