Structure of O-specific polysaccharide from Pseudoalteromonas nigrifaciens strain KMM 161.
On mild acid degradation of the lipopolysaccharide of the marine microorganism Pseudoalteromonas nigrifaciens KMM 161 an O-specific polysaccharide containing D-galactose, 2-acetamido-2-deoxy-D-glucose, 3,6-dideoxy-3-(4-hydroxybutyramido)-D-galactose, and 2-acetamido-2-deoxy-L-guluronic acid residues was obtained. From the results of Smith degradation, O-deacetylation of the polysaccharide, and NMR spectroscopy the following structure of the tetrasaccharide repeating unit of the O-specific polysaccharide was established [see reaction]. It should be noted that the same structure occurs in the antigenic polysaccharide of Pseudoalteromonas nigrifaciens KMM 158 described earlier as Alteromonas macleodii 2MM6.
Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia. elnaz@piboc.dvo.ru
R P
RP
Gorshkova
E L
EL
Nazarenko
V A
VA
Zubkov
E P
EP
Ivanova
N M
NM
Gorshkova
V V
VV
Isakov
D000942:Antigens, Bacterial / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D031161:Pseudoalteromonas / Q000737:chemistry* / Q000276:immunology
D000942:Antigens, Bacterial; D002247:Carbon Isotopes; D019081:O Antigens