Nuclear localization of non-specific acid phosphatase(s) activity in rat vaginal epithelium.
The nuclear localization of non-specific acid phosphatase(s) in rat vaginal epithelial cells (VEC) by histochemical and biochemical techniques has been reported in the present work. By different histochemical methods, the enzyme activity was predominantly localized in certain nuclei but not in all of them, however, within the nucleus nucleoli are free from the enzyme activity. This enzyme is diffused in the nucleoplasm and perhaps tightly associated with the nuclear proteins. Very little activity was seen in the cytoplasm in the selective population of VEC. After 12 h treatment of estradiol 17beta in vivo the VEC lose the enzyme activity in the basal and intermediate layers, and the activity remains within the nuclei of luminal cells. Enzyme assay in low and high salt extracts of the isolated nuclei of the VEC with or without the enzyme inhibitors indicates that this enzyme may be present in isoforms in the VEC. No activity was observed in these cells at alkaline pH.
Centre for Cellular and Molecular Biology, Hyderabad, India.
R
R
Kumaresan
P D
PD
Gupta
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002458:Cell Fractionation; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D014621:Vagina / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*
D004791:Enzyme Inhibitors; D004958:Estradiol; D000135:Acid Phosphatase