Cmk2, a novel serine/threonine kinase in fission yeast.
The cmk2 gene of Schizosaccharomyces pombe encodes a 504 amino acid protein kinase with sequence homology with the calmodulin-dependent protein kinase family. The cmk2(+) gene is not essential for cell viability but overexpression of cmk2(+) blocks the cell cycle at G2 phase and this inhibition is cdc2-dependent. The Cmk2 is a cytoplasmic protein expressed in a cell cycle-dependent manner, peaking at the G1/S boundary. Overexpression of Cmk2 suppresses fission yeast DNA replication checkpoint defects but not DNA damage checkpoint defects, suggesting that the G2 cell cycle arrest mediated by high levels of Cmk2 provides sufficient time to correct DNA replication alterations.
Departament de Biologia Cellular, Institut de Investigacions Biomèdiques August Pi i Sunyer, Facultat de Medicina, Universitat de Barcelona, E-08036 Barcelona, Spain.
Vicenç
V
Alemany
Maribel
M
Sanchez-Piris
Oriol
O
Bachs
Rosa
R
Aligue
D000595:Amino Acid Sequence; D001483:Base Sequence; D004249:DNA Damage; D017931:DNA Primers; D004261:DNA Replication; D016195:G2 Phase; D005800:Genes, Fungal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D017931:DNA Primers; D014443:Tyrosine; C459603:Cmk2 protein, S pombe; D017346:Protein Serine-Threonine Kinases