Plasmid DNA encoding the respiratory syncytial virus G protein protects against RSV-induced airway hyperresponsiveness.
Respiratory syncytial virus (RSV) is an important cause of childhood respiratory disease as well as exacerbations of asthma. Although previous studies have demonstrated that a DNA vaccine encoding the RSV G protein can inhibit RSV replication in mouse models of RSV infection, studies have not been performed to determine whether a DNA vaccine encoding the RSV G protein can protect against RSV induced mucus expression and airway hyperresponsiveness which was the focus of this study. The DNA-G vaccine we constructed significantly inhibited RSV viral replication, mucus expression, and importantly was associated with inhibition of RSV induced airway responsiveness.
Miller
Marina
M
Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0635, USA.
Cho
Jae Youn
JY
Baek
Kwang Je
KJ
Castaneda
Diego
D
Nayar
Jyothi
J
Rodriguez
Monica
M
Roman
Mark
M
Raz
Eyal
E
Broide
David H
DH
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D018793:Interleukin-13 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009093:Mucus / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000517:prevention & control; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D019444:Vaccines, DNA / Q000235:genetics / Q000494:pharmacology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000494:pharmacology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D018793:Interleukin-13; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines