DNA photocleavage by a supramolecular Ru(II)-viologen complex.
A novel Ru(II) complex possessing two sequentially linked viologen units, Ru-V(1)-V(2)(6+), was synthesized and characterized. Upon excitation of the Ru(II) unit (lambda(exc) = 532 nm, fwhm approximately 10 ns), a long-lived charge-separated (CS) state is observed (tau = 1.7 micros) by transient absorption spectroscopy. Unlike Ru(bpy)(3)(2+), which cleaves DNA upon photolysis through the formation of reactive oxygen species, such as (1)O(2) and O(2)(-), the photocleavage of plasmid DNA by Ru-V(1)-V(2)(6+) is observed both in air and under N(2) atmosphere (lambda(irr) > 395 nm).
Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA.
Patty K-L
PK
Fu
Patricia M
PM
Bradley
Dietmar
D
van Loyen
Heinz
H
Dürr
Stefan H
SH
Bossmann
Claudia
C
Turro
D004247:DNA / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D004587:Electrophoresis, Agar Gel; D015394:Molecular Structure; D009584:Nitrogen / Q000737:chemistry; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D010084:Oxidation-Reduction; D010777:Photochemistry; D010957:Plasmids; D012428:Ruthenium / Q000737:chemistry*; D014755:Viologens / Q000737:chemistry*
D009942:Organometallic Compounds; D014755:Viologens; D012428:Ruthenium; D004247:DNA; D009584:Nitrogen