Effects of dental resin components on vascular reactivity.
The frequent use of resins in dentistry has raised the question of their compatibility with oral tissues. The present study was undertaken to determine the effects of the resin components methyl methacrylate (MMA), hydroxyethyl methacrylate (HEMA), and triethylene glycol dimethacrylate (TEGDMA) on the reactivity of blood vessels using the isolated rat aorta as a tissue model. MMA, HEMA, and TEGDMA caused endothelium-dependent and -independent relaxation of rat aortic rings in a concentration-related manner. The endothelium-dependent responses of the tissues to all the resins were significantly attenuated by N-nitro-L-arginine methyl ester (L-NAME), indicating the involvement of nitric oxide. The vasorelaxant effects of both MMA and TEGDMA on the intact and denuded aortae were markedly inhibited by indomethacin, providing evidence for the role of prostanoids in these responses. Glybenclamide selectively attenuated TEGDMA-induced relaxation of the tissues with and without endothelium to a similar extent, suggesting the activation of vascular smooth muscle K(ATP) channels by this resin. It is concluded that MMA, HEMA, and TEGDMA interfere with the function of blood vessels by inducing vasorelaxation via different mechanisms, which, depending upon the type of resin, may at least involve the release of nitric oxide and prostanoid(s), and the activation of smooth muscle K(ATP) channels. These phenomena may play a role in tissue homeostasis and certain pathophysiological conditions associated with the application of resin materials to the oral environment.
Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, Georgia 30912-1128, USA.
William F
WF
Maddux
Worku
W
Abebe
George S
GS
Schuster
Mahmood S
MS
Mozaffari
D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism; D002317:Cardiovascular Agents / Q000494:pharmacology; D003188:Composite Resins / Q000494:pharmacology; D003764:Dental Materials / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005905:Glyburide / Q000494:pharmacology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008689:Methacrylates / Q000494:pharmacology*; D020366:Methylmethacrylate / Q000494:pharmacology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011109:Polymethacrylic Acids / Q000494:pharmacology*; D051381:Rats; D011921:Rats, Inbred WKY; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology*
D002317:Cardiovascular Agents; D003188:Composite Resins; D003764:Dental Materials; D004791:Enzyme Inhibitors; D008689:Methacrylates; D011109:Polymethacrylic Acids; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; C020946:triethylene glycol dimethacrylate; D020366:Methylmethacrylate; D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate; D005905:Glyburide; D019331:NG-Nitroarginine Methyl Ester; D009638:Norepinephrine; D007213:Indomethacin