A Novel Type of Apoptosis Induced by Cadmium in BA/F3beta Cells.
Apoptosis is usually accompanied by DNA fragmentation and up-regulation of reactive oxygen species, and it can be inhibited by overexpression of Bcl-2. Here, cadmium was found to induce apoptosis in BA/F3beta cells. MTT assay, Hochest 33258 staining, and transmission electron microscopy analysis were used to detect the apoptosis, however, neither DNA fragmentation nor up-regulation of reactive oxygen species were observed in this type of apoptosis. Furthermore, Bcl-2 overexpression had no effect on this type of apoptosis. In conclusion, these data suggested that cadmium induced a novel type of apoptosis in BA/F3beta cells.
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. sib203@sunm.shcnc.ac.cn
Qi-Wei
QW
Zhai
Hong-Bin
HB
Ji
Zhong-Cheng
ZC
Zheng
Lan-Ying
LY
Sun
Xin-Yuan
XY
Liu