Chromosomal assignments for porcine genes encoding enzymes in hepatic metabolic pathways.
Increasing the number of mapped genes will facilitate (1) the identification of potential candidate genes for a trait of interest within quantitative trait loci regions and (2) comparative mapping. The metabolic activities of the liver are essential for providing fuel to peripheral organs, for regulation of amino acid, carbohydrate and lipid metabolism and for homoeostasis of vitamins, minerals and electrolytes. We aimed to identify and map genes coding for enzymes active in the liver by somatic cell genetics in order to contribute to the improvement of the porcine gene map. We mapped 28 genes of hepatic metabolic pathways including six genes whose locations could be confirmed and 22 new assignments. Localization information in human was available for all but one gene. In total 24 genes were assigned to in the expected chromosomal regions on the basis of the currently available information on the comparative human and pig map while for four genes our results suggest a new correspondence or extended regions of conservation between porcine and human chromosomes.
Institute of Animal Breeding Science, University of Bonn, Bonn, Germany. wimmers@itz.uni-bonn.de
K
K
Wimmers
S
S
Ponsuksili
U
U
Bläser
J
J
Gellin
K
K
Schellander
D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary*; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D006801:Humans; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D040641:Quantitative Trait Loci; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000235:genetics*
D004798:Enzymes