Identification of a gene associated with astragalus and angelica's renal protective effects by silver staining mRNA differential display.
OBJECTIVE
To identify genes associated with the chronic progression of renal disease and a stragalus and angelica (A&A)'s renal protective effects.


METHODS
The technique of silver staining mRNA differential display (DD) was used to investigate changes of gene expression in normal, sclerotic and A&A treated sclerotic kidneys. We isolated genes differentially expressed during the progression of renal disease which could be normalized by A&A.


RESULTS
Several genes related to A&A's protective effects were isolated and one of them was confirmed by Northern blot.


CONCLUSIONS
Silver staining mRNA differential display is a simple and effective technique for isolating differentially expressed genes. The isolated new gene may be related to the progression of chronic renal disease and contribute to A&A's protective effects.
Department of Nephrology, First Teaching Hospital, Institute of Nephrology, Peking University, Beijing 100034, China.
Ling
L
Yu
Youyong
Y
Lu
Jingzi
J
Li
Haiyan
H
Wang
D029971:Angelica sinensis; D000818:Animals; D027883:Astragalus Plant; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D007674:Kidney Diseases / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008517:Phytotherapy; D016133:Polymerase Chain Reaction; D020011:Protective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling
D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D012333:RNA, Messenger