[An experimental study on effect of perfluorohexyloctane on corneal endothelial cells].
OBJECTIVE
To investigate the effect of perfluorohexyloctane on corneal endothelial cells in rabbit eyes.


METHODS
Fifteen New Zealand albino rabbits were divided into two groups: experiment group (F(6)H(8)) and control group (BSS). All rabbits underwent anterior chamber injection of 0.15 ml F(6)H(8) or BSS. Slit-lamp biomicroscopy and corneal endothelium photography were performed pre- and post-injection. Histopathological examination and transmission electron microscopy (TEM) were performed after the rabbits were sacrificed.


RESULTS
All the corneas were clear. Since 4 weeks postoperatively, the endothelial cells were markedly irregular in size and shape, and the number of endothelial cells was markedly decreased. Multilayered retrocorneal membranes grew gradually from 4 weeks after surgery. Vacuolar degeneration was seen in some endothelial cells. Nuclear degeneration and edema of endoplasmic reticulum were seen in TEM.


CONCLUSIONS
Corneal endothelial cells degenerated after contacting with F(6)H(8) for 4 weeks. As a silicone solvent, F(6)H(8) should be removed out completely after injection. It is not recommended to apply this agent as a new intraocular temponade.
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. xyding@163.net)
Xiaoyan
X
Ding
Chunfang
C
Li
Lin
L
Lu
Guanguang
G
Feng
Huling
H
Zheng
Guoming
G
Zhang
D000818:Animals; D002452:Cell Count; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D048429:Cell Size / Q000187:drug effects / Q000502:physiology; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000648:ultrastructure; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000473:pathology; D005466:Fluorocarbons / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects / Q000648:ultrastructure; D011817:Rabbits; D014617:Vacuoles / Q000187:drug effects / Q000648:ultrastructure
D005466:Fluorocarbons; C118616:perfluorohexyl-octan