Two-hybrid systems and their usage in infection biology.
Two-hybrid systems are powerful tools to study protein-protein interactions in biological systems. The role of protein-protein interactions involved in pathogenesis of bacterial and viral infections were defined by using yeast or bacterial two-hybrid screens. Examples are given to highlight the specificity of interactions in signaling pathways, in regulation, secretion and structure-function relationships of virulence factors and their cellular targets. Two-hybrid systems were also used to establish large-scale protein interaction maps of viral and bacterial pathogens, that might be useful to identify targets for new drugs or vaccines.
Karimova
Gouzel
G
Unité de Biochimie Cellulaire, CNRS URA 2185, Institut Pasteur, Paris, France.
Ladant
Daniel
D
Ullmann
Agnes
A
D001424:Bacterial Infections / Q000378:metabolism*; D023281:Genomics / Q000379:methods; D006801:Humans; D020798:Two-Hybrid System Techniques; D014777:Virus Diseases / Q000378:metabolism*