Sequencing of a 3.3 kb Fragment from LsNPV Genome and Comparison of Its Genome Structure with Other Two NPVs.
A fragment (3 336 bp) of LsNPV genome has been sequenced. It contains three complete ORFs (ORF2, ORF3, ORF4) and two incomplete ORFs (ORF1, ORF5). Comparing with AcNPV genome it was found that ORF1 coding product was homologous with P94 protein ORF3, ORF4 and ORF5 were homologous with ORF60, ORF59 and ORF57 of AcNPV respectively. Their transcriptional orientations are identical. It is suggested that the ORF2 is a new gene of LsNPV genome. The results showed that the genomic structure of LsNPV is quite different with that of other baculovirus compared. This may be helpful for investigation of the LsNPV molecular biology.
Institute of Virology Wuhan University Wuhan 430072 China.
De-Li
DL
Liu
Tian-Quan
TQ
Jin
Yi-Peng
YP
Qi
Li
L
Li