Effect of Acanthopanax giraldii Harms Var. Hispidus Hoo polysaccharides on the human gastric cancer cell line SGC-7901 and its possible mechanism.
OBJECTIVE
To study the inhibitory effect of Acanthopanax giraldii Harms Var. Hispidus Hoo polysaccharides (AGP) on SGC-7901 gastric cancer cells and its possible mechanism.


METHODS
Cell doubling time analysis, colony forming assay and MTT assay were adopted to study the inhibitory effect and its characteristics. We also analyzed the amount of protein expressed by oncogenes, antioncogenes and cell factors using flow cytometric analysis.


RESULTS
AGP inhibited the proliferation of SGC-7901 cells and cell colony forming ability. AGP did not inhibit the viability and function of lymphocytes of peripheral blood in healthy subjects and human embryonic tenocytes, except for the highest dosage of AGP (P < 0.05), which slightly inhibited the viability and function of the two types of normal cells. AGP inhibited the viability and function of SGC-7901 cells, except for the lowest dosages of AGP I and AGP III. There was a dose-effect relationship between the dosage of the AGP and SGC-7901 cells. The effect of the AGP at the molecular level was associated with the low protein expression of the c-myc and bcl-2 genes and the high protein expression of the p53, bax, fas and fas-L genes, as well as the cell factor TGF beta(1). The inhibitory effect of AGP was weaker than that of CDDP, but was stronger than that of Vitamin C.


CONCLUSIONS
Acanthopanax giraldii Harms Var. Hispidus Hoo polysaccharides selectively inhibited the proliferation, the colony forming ability, and the viability and function of human gastric cancer cells through the low protein expression of c-myc, bcl-2 and the high protein expression of p53, fas, fas-L and the cell factor TGF beta(1). The different inhibitory characteristics on the normal cells and cancer cells are possibly caused by gene and the cell factor expressions.
Institute of Military Medical Sciences, Chengdu Military Command, Kunming 650032, China. ruyilu@163.net
Xiaoying
X
Lu
Miancheng
M
Su
You
Y
Li
Linfu
L
Zeng
Xinghua
X
Liu
Jianming
J
Li
Baochun
B
Zheng
Shuangyin
S
Wang
D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004365:Drugs, Chinese Herbal; D011134:Polysaccharides