Multiple Rabaptin-5-like transcripts.
Rabaptin-5 is an important component of intracellular vesicular transport machinery. In this report, we describe a novel Rabaptin-5gamma transcript which has a 120 nt deletion in coding region of Rabaptin-5 messenger RNA (mRNA). We also cloned the 5'part of Rabaptin-5delta transcript and showed that upstream previously described deletion, Rabaptin-5delta mRNA is identical to Rabaptin-5. Both deletions are in-frame and result in excision of 40 and 43 aa, respectively, from Rabaptin-5 polypeptide chain. We also detected Rabaptin-5delta and Rabaptin-5gamma transcripts in human. The results of Southern blot analysis and human genome sequence analysis strongly suggest that these transcripts along with Rabaptin-5 and Rabaptin-4 are encoded by a single gene, RAB5EP, and differ due to alternative pre-mRNA splicing. Although Rabaptin-5delta and Rabaptin-5gamma transcripts are low-abundant compared to Rabaptin-5 mRNA, their ubiquitous expression patterns might point on significant roles played by encoded by them proteins.
Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov Street, Moscow 119334, Russia. alenavk@ibg.ac.ru
Elena V
EV
Korobko
Sergey L
SL
Kiselev
Igor V
IV
Korobko
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D005091:Exons; D015870:Gene Expression; D005796:Genes / Q000235:genetics; D006801:Humans; D007438:Introns; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D008565:Membrane Proteins; D020033:Protein Isoforms; C097227:RABEP1 protein, human; D012333:RNA, Messenger; C486276:Rabep1 protein, mouse; D033921:Vesicular Transport Proteins