Crystallization and preliminary X-ray crystallographic studies of the PDZ domain of Shank1 from Rattus norvegicus.
Shank proteins are a new family of scaffold proteins interacting with various membrane and cytoplasmic proteins. Shank contains multiple protein-protein interaction sites, including ankyrin repeats, an SH3 domain, a PDZ domain, a long proline-rich region and an SAM domain. The PDZ domain of Shank binds to the C-terminus of guanylate kinase-associated protein (GKAP). The PDZ domain of Shank1 from Rattus norvegicus and its complex with the C-terminal octapeptide of GKAP were crystallized at 294 K using polyethylene glycol 20 000 and 6000 as precipitants. Diffraction data sets from a peptide-free crystal and a complex crystal were collected to 1.8 and 3.2 A resolution, respectively, using synchrotron radiation. The peptide-free crystal belongs to space group P2(1), with unit-cell parameters a = 42.0, b = 50.3, c = 51.8 A, beta = 106.3 degrees. The complex crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 89.4, b = 97.5, c = 108.3 A.
Department of Life Science, Kwangju Institute of Science and Technology, Gwangju 500-712, South Korea.
Seong Ho
SH
Park
Young Jun
YJ
Im
Seong Hwan
SH
Rho
Jun Hyuck
JH
Lee
Soyoung
S
Yang
Eunjoon
E
Kim
Soo Hyun
SH
Eom
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D046911:Macromolecular Substances; D009419:Nerve Tissue Proteins / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D000074407:SAP90-PSD95 Associated Proteins
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D046911:Macromolecular Substances; D009419:Nerve Tissue Proteins; D009842:Oligopeptides; D011994:Recombinant Proteins; D000074407:SAP90-PSD95 Associated Proteins; C120531:Shank1 protein, rat