Cytosolic free Ca2+ concentration in canine aortic endothelial cells lining the polyester arterial prosthesis.
A rise in baseline cytosolic free Ca2+ in canine vascular endothelial-like cells (VEC) lining the luminal surface of the polyester arterial prosthesis is described. In one, three and six month implantation experiments we employed six adult mongrel dogs, polyester arterial prostheses Arteknit Ra K, fluorescent Ca2+ indicator Fura-2 and digital imaging microscopy to study cytosolic free Ca2+ in cultured VEC. The electron microscopy scanning of the luminal surface in different regions of the graft were also performed. A rise in cytosolic free Ca2+ in the VEC lining the luminal surface of the prosthesis is probably the result of the immunologic reaction and mechanical stress which stimulate the proliferation activity of the endothelial cells. It seems that the baseline cytosolic free Ca2+ reflects the course of the endothelization process on the polyester arterial prosthesis.
Department of Cardiac Surgery, F D Roosevelt Hospital, Banská Bystrica, Slovakia.
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P
Ondrus
R
R
Alberty
P
P
Pothier
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V
Echavé
J
J
Poisson
G
G
Bkaily
D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000601:surgery*; D001807:Blood Vessel Prosthesis; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division; D003600:Cytosol / Q000378:metabolism; D004285:Dogs; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000637:transplantation / Q000648:ultrastructure; D005260:Female; D005456:Fluorescent Dyes; D016257:Fura-2; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D013314:Stress, Mechanical
D005456:Fluorescent Dyes; D011091:Polyesters; D002118:Calcium; D016257:Fura-2