Polymerase chain reaction amplification of 16S-23S spacer region for rapid identification of Salmonella serovars.
Polymerase chain reaction (PCR) was used to amplify the spacer regions between the 16S and 23S genes of rRNA genetic loci of Salmonella serovars for their rapid identification. These genetic loci revealed a significant level of polymorphism in length across the species/serovar lines. When the 16S-23S spacer region amplification products were subjected to agarose electrophoresis, the patterns observed could be used to distinguish all the serovars of Salmonella tested. Unique elements obtained in amplification products were mostly clustered at serovar level, although certain genus-specific patterns were also observed. On the basis of the results obtained, the amplification of 16S-23S ribosomal spacer region could suitably be used in a PCR-based identification method for Salmonella serovars.
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, UP, India. shekhar1966@yahoo.com
C S
CS
Bakshi
V P
VP
Singh
Meenakshi
M
Malik
Bhaskar
B
Sharma
R K
RK
Singh
D000818:Animals; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000592:standards / Q000662:veterinary*; D011110:Polymorphism, Genetic; D011237:Predictive Value of Tests; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D012475:Salmonella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012481:Salmonella Infections, Animal / Q000175:diagnosis* / Q000235:genetics*; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000662:veterinary
D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S