Isolation of a novel peptide homing to tumor-derived neovasculature that suppresses tumor growth.
Novel peptides homing to angiogenic vessels were recently isolated from a phage-displayed random pentade-capeptide library, and peptides having WRP sequence showed tumor growth suppression. In this study, we observed that another novel sequence, PVVLFPLH, suppressed tumor growth in vivo. Through the study of tumor growth suppression by the 5-mer peptides derived from this sequence, we determined the epitope sequence to be LFPLH. LFPLH, but not the shuffled peptide FHLLP, suppressed the migration of vascular endothelial growth factor-stimulated human umbilical vein endothelial cells. Interestingly, growth suppression of LFPLH against the cells as well as tumor cells was not observed in vitro. Therefore LFPLH may function to induce tumor dormancy through inhibition of angiogenesis.
Asai
Tomohiro
T
Department of Medical Biochemistry, University of Shizuoka School of Pharmaceutical Sciences, Japan.
Fukatsu
Hideto
H
Kuromi
Koichi
K
Ogino
Koichi
K
Tanaka
Michinori
M
Oku
Naoto
N
Taki
Takao
T
D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D010446:Peptide Fragments / Q000737:chemistry / Q000627:therapeutic use; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000627:therapeutic use*; D012513:Sarcoma, Experimental / Q000098:blood supply / Q000188:drug therapy*; D014471:Umbilical Veins / Q000166:cytology
D020533:Angiogenesis Inhibitors; D010446:Peptide Fragments; D019151:Peptide Library; D010455:Peptides