Immunolocalization of glucose transporter 1 and 3 in the placenta: application to cytodiagnosis of Papanicolaou smear.
A positive immunostaining for glucose transporter 1 (GLUT1) was exclusively localized in microvilli on the free surface of syncytiotrophoblasts in the placenta. An enhanced immunoreaction for glucose transporter 3 (GLUT3) was elicited in the cell membrane of intermediate trophoblasts and cytotrophoblasts. Neither GLUT1 nor GLUT3 was positive in decidual cells and epithelial components from cervical dysplasia and carcinoma in situ. Cervicovaginal smears from six pregnant women containing atypical cells of unknown origin were subjected to immunocytochemical testing with antibodies against GLUT1 and GLUT3. Atypical cells in smears from two pregnant women were found to be positive for GLUT3 while no specific immunoreaction for GLUT1 was elicited, indicating their origin from either intermediate trophoblasts or cytotrophoblasts. Through the use of antibodies against vimentin and cytokeratin 17, GLUT3-negative atypical cells were further sorted into decidual cells and epithelial components from cervical dysplasia, respectively.
Department of Pathology, Wakayama Medical University, Wakayama, Japan.
Misako
M
Sato
Yasushi
Y
Nakamura
Tukumi
T
Sogawa
Qifeng
Q
Yang
Takeshi
T
Taniguchi
Emiko
E
Taniguchi
Tomoko
T
Kagiya
Misa
M
Nakamura
Ichiro
I
Mori
Kennichi
K
Kakudo
D002278:Carcinoma in Situ / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D002584:Cervix Uteri / Q000473:pathology; D003581:Cytodiagnosis; D003656:Decidua / Q000737:chemistry / Q000166:cytology; D005260:Female; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D006801:Humans; D007124:Immunoenzyme Techniques; D009004:Monosaccharide Transport Proteins / Q000032:analysis*; D009419:Nerve Tissue Proteins; D065006:Papanicolaou Test; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D013194:Staining and Labeling / Q000379:methods; D014327:Trophoblasts / Q000737:chemistry / Q000166:cytology; D002583:Uterine Cervical Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology
D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; C495677:SLC2A1 protein, human; C495687:SLC2A3 protein, human