Electrospray ionisation mass spectrometric detection of weak non-covalent interactions in nogalamycin-DNA complexes.
We demonstrate the use of electrospray ionisation mass spectrometry (ESI-MS) in high salt solutions for the analysis of weak non-covalent complexes of the anthracycline antibiotic nogalamycin with novel DNA hairpin structures; high signal-to-noise ratios for the complexes in the absence of bound Na+ ions permits relative binding affinities to be estimated.
Colgrave
Michelle L
ML
Department of Chemistry, University of Wollongong, NSW, 2522, Australia.
Beck
Jennifer L
JL
Sheil
Margaret M
MM
Searle
Mark S
MS
D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D007364:Intercalating Agents / Q000737:chemistry*; D009621:Nogalamycin / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D021241:Spectrometry, Mass, Electrospray Ionization
D007364:Intercalating Agents; D004247:DNA; D009621:Nogalamycin