Inductively coupled plasma mass spectrometric determination of molybdenum in urine.
A method was developed for the determination of molybdenum (Mo) in human urine by direct dilution of the sample in doubly distilled water with 1% HNO3 (v/v) and inductively coupled mass spectrometry (ICP-MS). In and Y were used as internal standards. Since (98)Mo provides a higher sensitivity, it was chosen as the reference isotope. The influence of different factors, such as sample dilution, HNO3 concentration and the stability of the analyte were evaluated. The detection limit (LOD) was assessed at 0.2 microg/L Mo, while the lower limit of quantification (LOQ) was 0.6 microg/L. Recoveries ranged between 97.2 and 100.7% from solutions containing from 10 to 50 microg/L Mo. Linear calibration curves were generated from 2.1 and 52.1 microg/L with coefficients of variation (CV ) ranging from 1.62 to 3.56%. In order to establish reference values (RV) for molybdenum, the procedure presented here was used to determine Mo in the urine of a population group living in Tuscany, Italy.
Laboratory of Environmental Hygiene and Industrial Toxicology, Salvatore Maugeri Foundation, via Ferrata 8, 27100 Pavia, Italy. igamb@fsm.it
Claudio
C
Minoia
Anna
A
Gatti
Cristina
C
Aprea
Anna
A
Ronchi
Gianfranco
G
Sciarra
Roberta
R
Turci
Maurizio
M
Bettinelli
D006801:Humans; D007554:Isotopes; D013058:Mass Spectrometry / Q000379:methods*; D008982:Molybdenum / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007554:Isotopes; D008982:Molybdenum