Estrogen receptor beta is involved in the anorectic action of estrogen.
OBJECTIVE
Estrogen has been implicated in feeding behavior and adiposity. This study was undertaken to elucidate the mechanism underlying the anti-obesity and anorectic action of estrogen and the role of estrogen receptor (ER) in the central nervous system.


RESULTS
Ovariectomy in 8-week-old female Wistar rats induced hyperphagia along with an increase in body weight and abdominal fat accumulation compared to control sham-operated rats. These changes were fully reversed by subcutaneous replacement of estradiol and were abrogated by pair-feeding. Then, the effects of intracerebroventricular infusion of estradiol, alone or in combination with antisense oligodeoxynucleotides (ODN), for ER in ovariectomized rats were examined. The estradiol group showed 10-20% lower daily food intake, and after the 2-week infusion period a 14% reduction in body weight with a similar reduction in abdominal fat compared to the vehicle group. The inhibitory effect of estradiol on food intake and body weight was blocked by co-administration of ER-beta antisense ODN, whereas ER-alpha antisense ODN did not show any influence.


CONCLUSIONS
These results indicate that ER-beta in the central nervous system is involved in the anorectic action of estrogen.
Department of Geriatric Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Y-Q
YQ
Liang
M
M
Akishita
S
S
Kim
J
J
Ako
M
M
Hashimoto
K
K
Iijima
Y
Y
Ohike
T
T
Watanabe
N
N
Sudoh
K
K
Toba
M
M
Yoshizumi
Y
Y
Ouchi
D000273:Adipose Tissue / Q000187:drug effects; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D004334:Drug Administration Schedule; D004435:Eating / Q000187:drug effects; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D047629:Estrogen Receptor beta; D005260:Female; D007276:Injections, Intraventricular; D009765:Obesity / Q000378:metabolism*; D020319:Oligodeoxyribonucleotides, Antisense / Q000008:administration & dosage / Q000494:pharmacology; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar; D011960:Receptors, Estrogen / Q000378:metabolism*
D047629:Estrogen Receptor beta; D020319:Oligodeoxyribonucleotides, Antisense; D011960:Receptors, Estrogen; D004958:Estradiol