[Clinical significance of expression of the smooth muscle actin-alpha and CD34 antigen in mesangial cells in glomerulonephritis].
OBJECTIVE
To assess clinical implications of expression of alpha-smooth-muscle actin (ASMA) and CD34 as markers of activation and transformation of mesangial cells.


METHODS
ASMA and CD34 expression was investigated with indirect enzyme immunoassay with addition of the complex alkaline phosphatase-antiphosphatase, mean number of stained (expressing) mesangial cells per 1 glomerulus were estimated in 47 renal tissue biopsies from patients with different morphological types of glomerulonephritis and 9 biopsies of the kidneys removed for different tumors (control).


RESULTS
Mean expression of ASMA (11.0 +/- 3.5) was significantly higher in GN proliferative types than in non-proliferative ones and controls. In patients with proliferative nephritis ASMA expression correlated with clinical signs of nephritis activity. CD34 was expressed similarly in patients with proliferative and non-proliferative nephritis and in controls. CD34 was higher in biopsies with higher degree of sclerosis in the kidney than in the biopsies with moderate sclerosis.


CONCLUSIONS
ASMA and CD34 expression by mesangial cells in renal biopsies from GN patients can be used for evaluation of the disease activity and prognosis.
Chebotareva
N V
NV
Proppe
D
D
Rudolf
P
P
Kozlovskaia
L V
LV
D000199:Actins / Q000378:metabolism*; D018952:Antigens, CD34 / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D005920:Glomerular Mesangium / Q000378:metabolism* / Q000473:pathology; D005921:Glomerulonephritis / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D009130:Muscle, Smooth / Q000378:metabolism*
D000199:Actins; D018952:Antigens, CD34; D015415:Biomarkers