Quantitative analysis of substance P, neurokinin A and calcitonin gene-related peptide in gingival crevicular fluid associated with painful human teeth.
The aim of this study was to investigate the presence of substance P (SP), neurokinin A (NKA) and calcitonin gene-related peptide (CGRP) in the gingival crevicular fluid of teeth diagnosed with pain of pulpal origin compared with clinically healthy teeth, and to detect any changes in the levels of these neuropeptides in gingival crevicular fluid after removal of the pulp from the painful teeth. Gingival crevicular fluid was collected at baseline from one interproximal site at a painful and a non-painful contralateral tooth from 54 adult patients. Sampling was repeated after 1 wk in a subset of 21 subjects. Samples were analysed for SP, NKA, and CGRP using radioimmunoassay. The mean levels of SP and NKA were significantly higher in gingival crevicular fluid from painful teeth compared with non-painful teeth. The level of SP in the GCF of painful teeth fell significantly 1 wk after pulpectomy. In contralateral teeth, there were no significant differences in the levels of SP and NKA after 1 wk. It is concluded that SP and NKA are present in significantly greater amounts in the GCF of painful teeth compared with healthy teeth.
Faculty of Dentistry, Department of Conservative Dentistry, Jordan University of Science and Technology, Irbid.
Lama A
LA
Awawdeh
Fionnuala T
FT
Lundy
Gerard J
GJ
Linden
Chris
C
Shaw
John G
JG
Kennedy
Philip-John
PJ
Lamey
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D003782:Dental Pulp / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D015288:Neurokinin A / Q000032:analysis*; D010512:Periodontal Index; D011670:Pulpectomy; D011671:Pulpitis / Q000378:metabolism* / Q000628:therapy; D011863:Radioimmunoassay; D012737:Sex Factors; D013223:Statistics as Topic; D018709:Statistics, Nonparametric; D013373:Substance P / Q000032:analysis*
D013373:Substance P; D015288:Neurokinin A; D015740:Calcitonin Gene-Related Peptide