Expression and binding properties of the two mannose-6-phosphate receptors differ during perinatal development in rat liver.
Mammalian tissues express both cation-dependent (CD-MPR) and cation-independent (CI-MPR) mannose-6-phosphate receptors, which mediate the targeting of acid hydrolases to lysosomes. The coexistence of the two receptors in all cell types and tissues is still poorly understood. To determine whether these receptors might play a role in maturation, we studied their expression and binding properties in rat liver during perinatal development. CI-MPR expression decreases progressively from 18-day fetuses to adults, whereas the CD-MPR showed a transient decrease in newborn and at the 5th day after birth. Immunostaining of the tissues showed that both receptors localize to hepatocytes at all the ages and, additionally, the CD-MPR was reactive in megakaryocytes at early stages. Binding assays showed differences in the B(max) and K(D) values between the ages studied. These results demonstrate that both receptors change differentially during perinatal development, suggesting that they play distinct roles during organ maturation.
Romano
Patricia S
PS
Facultad de Ciencias Médicas, Instituto de Histología y Embriología (IHEM), Universidad Nacional de Cuyo (Argentina), Casilla de Correo 56-(5500), Mendoza, Argentina.
López
Ana C
AC
Mariani
María L
ML
Sartor
Tirso
T
Belmonte
Silvia A
SA
Sosa
Miguel A
MA
D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D002455:Cell Division; D004305:Dose-Response Relationship, Drug; D018507:Gene Expression Regulation, Developmental; D015151:Immunoblotting; D007150:Immunohistochemistry; D007700:Kinetics; D008099:Liver / Q000196:embryology* / Q000254:growth & development*; D008247:Lysosomes / Q000378:metabolism; D008533:Megakaryocytes / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D017527:Receptor, IGF Type 2 / Q000096:biosynthesis* / Q000737:chemistry*; D013997:Time Factors
D017527:Receptor, IGF Type 2