The chemokine ESkine/CCL27 displays novel modes of intracrine and paracrine function.
We have previously shown that the beta-chemokine ESkine/CCL27 is differentially spliced to produce two alternative forms. One is a secreted chemokine (ESkine), whereas the other (PESKY) lacks a signal peptide and is translocated to the nucleus. The role of this nuclear-targeted chemokine has not so far been defined, and it was the purpose of this study to examine this chemokine variant in more depth. To identify the region of PESKY involved in the nuclear translocation we tagged fragments with enhanced green fluorescent protein and expressed them in Chinese hamster ovary cells. We show PESKY nuclear translocation to be dependent on C-terminal residues that are shared with the signal peptide-bearing variant ESkine. Indeed we further demonstrate that ESkine can also use these C-terminal residues to enter the nucleus of cells following receptor (CCR10)-mediated internalization. To examine biological roles for PESKY we have overexpressed it in 3T3 cells. Such overexpression results in marked cytoskeletal rearrangements that are coincident with a radical reorganization of the cellular actin cytoskeleton. Microarray analyses and Ab neutralization studies indicate that these changes are mediated in part by insulin-like growth factor-1. Furthermore, monolayer wounding assays indicate that PESKY expression correlates with markedly increased migratory capacity. Thus, it is our contention that nuclear PESKY and ESkine both enter the nucleus by either intracrine or paracrine mechanisms and may facilitate cellular migration by inducing actin cytoskeletal relaxation. Therefore, nuclear ESkine/PESKY represents a novel paradigm for chemokine function.
Gortz
Andreas
A
Beatson Institute for Cancer Research, Cancer Research U.K. Beatson Laboratories, Bearsden, Glasgow, United Kingdom.
Nibbs
Robert J B
RJ
McLean
Pauline
P
Jarmin
David
D
Lambie
Wendy
W
Baird
Janet W
JW
Graham
Gerard J
GJ
D016475:3T3 Cells; D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D054425:Chemokine CCL27; D019742:Chemokines, CC / Q000737:chemistry / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data
D000199:Actins; C119538:Ccl27a protein, mouse; D054425:Chemokine CCL27; D019742:Chemokines, CC; D007334:Insulin-Like Growth Factor I