Peri-OVLT E-series prostaglandins and core temperature do not increase after intravenous IL-1beta in pregnant rats.
Rats have an attenuated febrile response to endogenous pyrogen near the term of pregnancy. Given the fundamental role of E-series prostaglandins (PGEs) in mediating the febrile response to blood-borne endogenous pyrogen, the present experiments were carried out to determine whether PGEs increase in the area surrounding the organum vasculosum laminae terminalis (peri-OVLT) of near-term pregnant (P) rats as in nonpregnant (NP) rats after intravenous (iv) administration of recombinant rat interleukin-1beta (rrIL-1beta). Core temperature was measured by telemetry and peri-OVLT interstitial fluid was sampled in 12 NP and 12 P chronically instrumented, Sprague-Dawley rats by microdialysis for determination of total PGEs by radioimmunoassay. Basal core temperatures were higher in NP compared with P rats (NP 37.9 degrees C +/- 0.5, P 36.9 degrees C +/- 0.4; P < 0.05), but basal peri-OVLT PGEs were similar in both groups (NP 260 +/- 153 pg/ml, P 278 +/- 177 pg/ml; P =not significant). Intravenous administration of rrIL-1beta to NP rats produced a significant increase in core temperature with a latency, magnitude, and duration of 10 min, 0.87 degrees C, and at least 170 min, respectively; peri-OVLT PGEs were increased significantly by 30 min and averaged 270% above basal levels throughout the experiment. In P rats, however, neither core temperature nor peri-OVLT PGEs increased significantly after iv administration of rrIL-1beta. Intravenous administration of vehicle did not significantly alter core temperature or peri-OVLT PGEs in either group of rats. Thus peri-OVLT PGEs do not increase in P rats as they do in NP rats after iv administration of rrIL-1beta. The mechanism of this interesting component of the maternal adaptation to pregnancy, which likely plays a major role in mediating the attenuated febrile response to endogenous pyrogen near the term of pregnancy, warrants further investigation.
Fewell
James E
JE
Department of Physiology, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N1. fewell@ucalgary.ca
Eliason
Heather L
HL
Auer
Roland N
RN
D000818:Animals; D001831:Body Temperature / Q000187:drug effects / Q000276:immunology*; D005260:Female; D007031:Hypothalamus / Q000276:immunology / Q000378:metabolism; D007275:Injections, Intravenous; D007375:Interleukin-1 / Q000494:pharmacology*; D017551:Microdialysis; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D011458:Prostaglandins E / Q000378:metabolism*; D011749:Pyrogens / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D007375:Interleukin-1; D011458:Prostaglandins E; D011749:Pyrogens