Impaired maturation of the siderophore pyoverdine chromophore in Pseudomonas fluorescens ATCC 17400 deficient for the cytochrome c biogenesis protein CcmC.
Pyoverdines are the main siderophores of fluorescent pseudomonads. They comprise a quinoline chromophore, a peptide chain, and a dicarboxylic acid or a dicarboxylic acid amide side chain. Each Pseudomonas species produces a pyoverdine with a different peptide chain. A cytochrome c biogenesis DeltaccmC mutant of Pseudomonas fluorescens ATCC 17400 produces multiple pyoverdine forms, showing differences at the level of the chromophore or the side chain. When grown in the presence of L-cysteine, DeltaccmC produces only ferribactin, a non-fluorescent precursor of pyoverdine, while addition of oxidized glutathione improves pyoverdine production. We suggest that the conversion of ferribactin to pyoverdine does not take place in the DeltaccmC mutant because of lack of oxidizing power in the periplasm.
Laboratory of Microbial Interactions, Department of Immunology, Parasitology and Ultrastructure, Flanders Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, Paardenstraat 65, B-1640, Sint-Genesius Rode, Belgium.
Christine
C
Baysse
Herbert
H
Budzikiewicz
Diana
D
Uría Fernández
Pierre
P
Cornelis
D001426:Bacterial Proteins; D003545:Cysteine / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009842:Oligopeptides; D019897:Periplasm / Q000378:metabolism; D010860:Pigments, Biological / Q000302:isolation & purification / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D017262:Siderophores / Q000302:isolation & purification / Q000378:metabolism
D001426:Bacterial Proteins; C116246:CcmC protein, bacteria; D008565:Membrane Proteins; D009842:Oligopeptides; D010860:Pigments, Biological; D017262:Siderophores; C042453:pyoverdin; D003545:Cysteine; D019803:Glutathione Disulfide