Effects of human neutrophil peptide-1 on the expression of interstitial collagenase and type I collagen in human dermal fibroblasts.
Human neutrophil peptide-1 (HNP-1), a defensin with antimicrobial properties, is also thought to promote wound healing. To elucidate the mechanism by which wound healing is facilitated by this factor, we investigated the effect of HNP-1 on the expression of interstitial collagenase (matrix metalloproteinase 1, MMP-1), collagen types I and III, and tissue inhibitor of metalloproteinase 1 (TIMP-1) by cultured fibroblasts by means of RT-PCR and ELISA. Our results showed that synthetic HNP-1 increased the expression of proalpha1(I) collagen mRNA and protein. In contrast, the expression of MMP-1 was decreased at both the mRNA and protein levels. Our observations suggest that HNP-1 may promote wound repair by enhancing extracellular matrix deposition and by controlling its degradation.
Department of Dermatology, Okayama University Graduate School of Medicine and Dentistry, Shikata-cho, 2-5-1, Okayama 700-8558, Japan. otakashi@cc.okayama-u.ac.jp
Takashi
T
Oono
Yoshinori
Y
Shirafuji
Wook-Kang
WK
Huh
Hisanori
H
Akiyama
Keiji
K
Iwatsuki
D001483:Base Sequence; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics* / Q000378:metabolism*; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism; D023084:alpha-Defensins / Q000494:pharmacology*
D024042:Collagen Type I; D024061:Collagen Type III; D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019715:Tissue Inhibitor of Metalloproteinase-1; D023084:alpha-Defensins; C406413:human neutrophil peptide 1; D020781:Matrix Metalloproteinase 1