Direct detection of singlet oxygen via its phosphorescence from cellular and fungal cultures.
In summary, we have developed methods to detect 1O2 spectrally from viable keratinocytes and fungal mycelial cultures. Both time-resolved and steady-state spectrophotometers were used, providing complementary information on optimal conditions for the unambiguous detection of 1O2 phosphorescence. By using our techniques, we were able to confirm that photosensitizers in contact with living cells indeed generate 1O2. The model systems and the procedures described can be adopted for other studies involving 1O2 and oxidative stress in living cells.
Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA.
Piotr
P
Bilski
Margaret E
ME
Daub
Colin F
CF
Chignell
D001203:Ascomycota / Q000378:metabolism; D001671:Biochemistry / Q000379:methods; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D008163:Luminescent Measurements; D010100:Oxygen / Q000378:metabolism*; D013053:Spectrophotometry / Q000379:methods; D013997:Time Factors
D010100:Oxygen