[The study of pro-nucleating activity on 33.5 x 10(3) vesicular protein in bile].
OBJECTIVE
To evaluate the pro-nucleating activity in 33.5 x 10(3) vesicular protein.


METHODS
The model biles were established according Kibe and Zhu. The pro-nucleating activity of 33.5 x 10(3) vesicular protein were examined by polarized light microscopy. The protein and its enzymatic deglycosylation and proteolysis fractions nucleation promoting activity were detected by cholesterol crystal growth assay.


RESULTS
33.5 x 10(3) vesicular protein displayed apparent potency of pro-nucleation with activity of 0.310, and derived crystal growth curve indices It, Ig, Ic were presented as 0.57, 1.52, 1.63 respectively, but after treated by N-glycanase enzyme and pronase, no promoting activity were found.


CONCLUSIONS
The 33.5 x 10(3) vesicular protein may be involved in the nucleation process of gallstone formation, which is regulated by its peptide and sugar chain.
Ma
Baojin
B
Department of Surgery, Huashan Hospital of Fudan University, Shanghai 200040, China.
Xiang
Jianbin
J
Cai
Duan
D
Zhang
Yanling
Y
Zha
Xiliang
X
Wang
Liying
L
D001646:Bile / Q000378:metabolism*; D002769:Cholelithiasis / Q000503:physiopathology*; D002784:Cholesterol / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D008853:Microscopy; D008954:Models, Biological; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism
D046911:Macromolecular Substances; D011506:Proteins; D002784:Cholesterol