Light-dependent changes in the chick pineal temperature and the expression of cHsp90 alpha gene: a potential contribution of in vivo temperature change to the photic-entrainment of the chick pineal circadian clock.
The circadian clock is entrained to the diurnal alteration of environmental conditions such as light and temperature, but the molecular mechanism underlying the entrainment is not fully understood. In the present study, we employed a differential display-based screening for a set of genes that are induced by light in the chick pineal gland, a structure of the central clock entrainable to both light and temperature changes. We found that the level of the mRNA encoding chicken heat shock protein 90 alpha (cHSP90 alpha) was rapidly elevated in the pineal gland within a 5-min exposure of chicks to light. Furthermore, the pineal cHsp90 alpha mRNA was expressed rhythmically under both 12-hr light/12-hr dark (LD) cycles and constant dark (DD) conditions. The total amount of the pineal cHSP90 alpha protein was, however, kept at nearly constant levels under LD cycles, and immunohistochemical analyses of the pineal cHSP90 alpha showed invariable localization at the cytoplasm throughout the day. In vivo measurement of the chick pineal temperature demonstrated its light-dependent and time-of-day-dependent change, and the profile was very similar to that of the pineal cHSP90 alpha mRNA level. These observations suggest that the in vivo temperature change regulates the expression of temperature-responsive genes including cHSP 90 alpha in the pineal gland. The temperature change may induce a phase-shift of the pineal clock, thereby facilitating its efficient entrainment to environmental LD cycles.
Doi
Masao
M
Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
Nakajima
Yoshito
Y
Okano
Toshiyuki
T
Fukada
Yoshitaka
Y
D000818:Animals; D001831:Body Temperature; D002645:Chickens / Q000378:metabolism*; D002940:Circadian Rhythm / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects*; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D008027:Light; D010870:Pineal Gland / Q000378:metabolism* / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D018841:HSP90 Heat-Shock Proteins; D012333:RNA, Messenger