Anionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells.
Toxoplasma gondii invades and proliferates in human umbilical vein endothelial cells where it resides in a parasitophorous vacuole. In order to analyze which components of the endothelial cell plasma membrane are internalized and become part of the parasitophorous vacuole membrane, the culture of endothelial cells was labeled with cationized ferritin or UEA I lectin or anti Class I human leukocyte antigen (HLA) before or after infection with T. gondii. The results showed no cationized ferritin and UEA I lectin in any parasitophorous vacuole membrane, however, the Class I HLA molecule labeling was observed in some endocytic vacuoles containing parasite until 1 h of interaction with T. gondii. After 24 h parasite-host cell interaction, the labeling was absent on the vacuolar membrane, but presents only in small vesicles near parasitophorous vacuole. These results suggest the anionic site and fucose residues are excluded at the time of parasitophorous vacuole formation while Class I HLA molecules are present only on a minority of Toxoplasma-containing vacuoles.
Stumbo
Ana Carolina
AC
Laboratório de Cultura de Células, Departamento de Histologia e Embriologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, 20550-170, Brasil.
Barbosa
Helene Santos
HS
Carvalho
Técia Maria Ulisses de
TM
Porto
Luís Cristóvão
LC
Carvalho
Laís de
Ld
D000818:Animals; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000276:immunology / Q000469:parasitology* / Q000648:ultrastructure; D005057:Eukaryotic Cells / Q000737:chemistry / Q000276:immunology; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D051379:Mice; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D014122:Toxoplasma / Q000276:immunology / Q000502:physiology*; D014470:Umbilical Cord / Q000276:immunology / Q000469:parasitology* / Q000648:ultrastructure; D014617:Vacuoles / Q000737:chemistry / Q000469:parasitology* / Q000648:ultrastructure
D015395:Histocompatibility Antigens Class I