Determination of lecithin drug specialties and diet integrators; by means of first or second derivative enzymatic-spectrophotometric analysis.
The possibility has been investigated of applying derivative analysis to a classical enzymatic-spectrophotometric method for lecithin determination for the purpose of developing an analytical direct method that does not require long pretreatment of the test sample even in the case of turbid samples. Several samples of drugs and food integrators containing lecithin were thus tested using both the standard and the derivative method and then comparing the results obtained. The RSD% values of measurements on real (food or drugs) samples were always <2.5. Using a first derivative spectrophotometric method, average recovery was always between 102 and 105%.
Department of Chemistry, University of Rome La Sapienza, Piazzale Aldo Moro 5, Rome, Italy.
L
L
Campanella
A L
AL
Magrì
A
A
Sorbo
M
M
Tomassetti
D000429:Alcohol Oxidoreductases / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D009195:Peroxidase / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010713:Phosphatidylcholines / Q000032:analysis*; D010739:Phospholipase D / Q000737:chemistry; D015203:Reproducibility of Results; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D004364:Pharmaceutical Preparations; D010713:Phosphatidylcholines; D012997:Solvents; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D009195:Peroxidase; D010739:Phospholipase D