Degradation of 4-chlorophenol by the white rot fungus Phanerochaete chrysosporium in free and immobilized cultures.
4-Chlorophenol (4-CP) degradation was investigated by suspended and immobilized Phanerochaete chrysosporium conducted in static and agitated cultures. The best results were achieved when experiment was carried out in a rotating biological contactor instead of an Erlenmeyer flask, for both batch degradation and repeated batch degradation. The relative contribution of lignin peroxidase (LiP) versus manganese peroxidase (MnP) to the 4-CP degradation by P. chrysosporium was investigated. 4-CP degradation slightly increased and a high level of MnP (38 nKat ml(-1)) was produced when P. chrysosporium was grown at high Mnll concentration. High LiP production in the medium had no significant effect on 4-CP degradation. 4-CP degradation occurred when P. chrysosporium was grown in a medium that repressed LiP and MnP production. This result indicates that LiP and MnP are not directly involved in 4-CP degradation by P. chrysosporium.
Environmental Biotechnology Unit, CBS, BP K, Sfax, Tunisia.
Hèla
H
Zouari
Marc
M
Labat
Sami
S
Sayadi
D002733:Chlorophenols / Q000378:metabolism*; D006868:Hydrolysis; D010544:Peroxidases / Q000378:metabolism; D020075:Phanerochaete / Q000201:enzymology / Q000378:metabolism*
D002733:Chlorophenols; C029107:4-chlorophenol; D010544:Peroxidases; C051129:manganese peroxidase