Expression of 5-HT1A receptor mRNA in rat lumbar spinal dorsal horn neurons after peripheral inflammation.
The present study observed the expression of the 5-hydroxytryptamine (5-HT) (1A) receptor mRNA in the lumbar spinal dorsal horn neurons following carrageenan inflammation using in situ hybridization (ISH). We also studied the co-localization of 5-HT(1A) receptor mRNA and gamma-amino butyric acid (GABA) or enkephalin (ENK) immunoreactivities using a combined fluorescent ISH and immunofluorescent histochemical double-staining technique. The finding of this study demonstrated that 5-HT(1A) receptor mRNA was widely distributed in the spinal dorsal horn with the highest density in laminae III-VI. Following carrageenan-induced inflammation, the 5-HT(1A) receptor mRNA expression in all layers of ipsilateral dorsal horn was significantly enhanced, and the peak occurred after 8h. Furthermore, the number of 5-HT(1A) receptor mRNA and GABA or ENK immunoreactive double-labeled cells was also markedly increased 8h after carrageenan injection. These findings suggested that following peripheral inflammation, the synthesis of 5-HT(1A) receptor was increased in the lumbar spinal dorsal horn neurons, especially in spinal GABA and ENK neurons.
Zhang
Yu-Qiu
YQ
Institute of Neurobiology, Fudan University, 220 Han Dan Road, Shanghai, 200433, People's Republic of China State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yi Xue Yuan Road, Shanghai, 200032, People's Republic of China.
Gao
Xiu
X
Ji
Guang-Chen
GC
Huang
Ya-Lin
YL
Wu
Gen-Cheng
GC
Zhao
Zhi-Qi
ZQ
D000818:Animals; D004745:Enkephalins / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D008161:Lumbosacral Region; D008297:Male; D010525:Peripheral Nerves / Q000378:metabolism / Q000473:pathology; D020671:Posterior Horn Cells / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000096:biosynthesis* / Q000235:genetics; D044263:Receptors, Serotonin, 5-HT1; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D004745:Enkephalins; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D044263:Receptors, Serotonin, 5-HT1; D005680:gamma-Aminobutyric Acid