Selective adsorption of serum albumin on biomedical polyurethanes modified by a poly(ethylene oxide) coupling-polymer with cibacron blue (F3G-A) endgroups.
A tri-block-coupling polymer, "PEO-MDI-PEO" ["poly(ethylene oxide)-4,4'-methylene diphenyl diisocyanate-poly(ethylene oxide)", abbreviated "MPEO"], was used to react with a triazine dye, Cibacron Blue F3G-A (ciba), in an alkaline environment. The product of this nucleophilic reaction was a penta-block-coupling polymer, "ciba-PEO-MDI-PEO-ciba" (abbreviated "cibaMPEO"). The cibaMPEO-modified poly(ether urethane) (PEU) surfaces were prepared by dip-coating and detected by XPS. The surface enrichment of both ciba endgroups and poly(ethylene oxide) spacer-arms was revealed. On the modified surfaces, bovine serum albumin (BSA)-adsorbing experiments were carried out, respectively, in the low and high BSA bulk-concentration solutions, and accordingly, the methods of radioactive (125)I-probe and ATR-FTIR were, respectively, employed for the characterization. The competitive adsorption of BSA and bovine serum fibrinogen (Fg) in the BSA-Fg binary solutions was also studied using a (125)I-probe, and through which the reversibly BSA-selective adsorption on cibaMPEO-modified PEU surfaces was confirmed. Finally, the improvement of blood-compatibility on the modified surfaces was verified by the plasma recalcification time (PRT) test.
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, PR China. danielwda@netzero.net
Dong-an
DA
Wang
Bao-lin
BL
Chen
Jian
J
Ji
Lin-xian
LX
Feng
D000327:Adsorption; D000818:Animals; D001780:Blood Coagulation Tests; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000493:pharmacokinetics*; D003432:Cross-Linking Reagents / Q000737:chemistry; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis; D011140:Polyurethanes / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013057:Spectrum Analysis; D013499:Surface Properties; D014227:Triazines / Q000737:chemistry
D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents; D011108:Polymers; D011140:Polyurethanes; D014227:Triazines; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; C012842:Cibacron Blue F 3GA; D005340:Fibrinogen