A WEE1 homologue from Arabidopsis thaliana.
Little is known about the genes that regulate cyclinB-Cdc2 complexes at the G2/M transition of the plant cell cycle although in yeast and animals cdc25 and wee1 are central regulators of cdc2. Here we describe the isolation, by reverse transcription polymerase chain reaction (RT-PCR), of a WEE1 cDNA (AtWEE1) in Arabidopsis thaliana (L.) Heynh. Semi-quantitative RT-PCR showed that AtWEE1 expression was confined to actively dividing regions of the plant. The overexpression of AtWEE1 in fission yeast (Schizosaccharomyces pombe) caused cells to arrest, and to grow but not divide, resulting in very elongated cells. Our data provide evidence for a functional WEE1 in A. thaliana.
Sorrell
David A
DA
School of Biosciences, Cardiff University, UK.
Marchbank
Angela
A
McMahon
Kathryn
K
Dickinson
J Richard
JR
Rogers
Hilary J
HJ
Francis
Dennis
D
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002455:Cell Division; D017931:DNA Primers; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012568:Schizosaccharomyces / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D017931:DNA Primers; D009687:Nuclear Proteins; D010940:Plant Proteins; D029702:Schizosaccharomyces pombe Proteins; C068689:wee1 protein, S pombe; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; C514674:WEE1 protein, Arabidopsis