Simultaneous determination of zidovudine and nevirapine in human plasma by RP-LC.
A simple method for the simultaneous determination of zidovudine and nevirapine in human plasma by reversed-phase liquid chromatography with UV detection at 265 nm was developed. A solid-liquid extraction procedure with internal standard was applied to the samples prior to analysis. The system requires a Zorbax SB-C18 column, 250 x 4.6 mm I.D. and a mobile phase composed of potassium dihydrogen phosphate (10 mM; pH 6.5)-acetonitrile (83:17, v/v). Peak-areas are linear; correlation coefficients are better than 0.999; both inter- and intra-day accuracy and precision are lower than 15%. Extraction recoveries are higher than 90% for both zidovudine and nevirapine. The method proposed was employed to determine the levels of the two retroviral drugs in plasma from HIV infected human subjects.
Marchei
Emilia
E
Istituto Superiore di Sanità, Viale Regina Elena 299, Rome, Italy.
Valvo
Luisa
L
Pacifici
Roberta
R
Pellegrini
Manuela
M
Tossini
Gianna
G
Zuccaro
Piergiorgio
P
D019380:Anti-HIV Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D019829:Nevirapine / Q000097:blood*; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000097:blood*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D015215:Zidovudine / Q000097:blood*
D019380:Anti-HIV Agents; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine; D019829:Nevirapine