gyrA polymorphism in Campylobacter jejuni: detection of gyrA mutations in 162 C. jejuni isolates by single-strand conformation polymorphism and DNA sequencing.
Mutations in the quinolone resistance-determining region of the gyrA gene from 138 ciprofloxacin-resistant (MIC, > or =4 microg/ml) and 24 ciprofloxacin-susceptible (MIC, < or =1 microg/ml) clinical Campylobacter jejuni isolates were subjected to single-strand conformation polymorphism analysis and sequencing. All of the isolates could be assigned to three genotypic clusters based on silent mutations. All resistant isolates had a point mutation at codon 86.
Hakanen
Antti
A
Antimicrobial Research Laboratory, National Public Health Institute, PO Box 57, 20521 Turku, Finland. antti.hakanen@utu.fi
Jalava
Jari
J
Kotilainen
Pirkko
P
Jousimies-Somer
Hannele
H
Siitonen
Anja
A
Huovinen
Pentti
P
D000890:Anti-Infective Agents / Q000494:pharmacology; D001345:Autoradiography; D002169:Campylobacter Infections / Q000382:microbiology; D016123:Campylobacter jejuni / Q000235:genetics*; D002939:Ciprofloxacin / Q000494:pharmacology; D027081:DNA Gyrase / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial; D005387:Finland; D005838:Genotype; D009154:Mutation / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational
D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D002939:Ciprofloxacin; D027081:DNA Gyrase