Identification of caseins in goat milk.
The importance of goat milk in infant diet is growing, because it is reported that goat's milk in some cases is less allergenic than cow's milk. This is due probably to the lower presence of caseins associated with a specific type of alpha(s1)-casein. In caprine breeds, four types of alpha(s1)-casein alleles are identified and associated with various amounts of this protein in milk. The contribution of strong alleles to the goat milk is approximately 3.6 g/L of alpha(s1)-casein, while for middle alleles is only 1.6 g/L, weak alleles 0.6 g/L. The contribution of null allele is very low (or non-existent). The quantity of total caseins in caprine milk is positively correlated with the amount of alpha(s1)-casein. Milk from animals possessing strong alleles contain significantly more total caseins than milk from animals without those alleles. This is important because animals with mild alleles can be employed to produce milk for allergic subjects while the other animals can be used to produce milk for the dairy industry. This work shows casein profiles of two types of classified goat milk (B, strong alpha(s1) allele, 0, null alpha(s1) allele) with two-dimensional electrophoresis coupled with matrix-assisted laser desorption/ionization-time of flight mass spectrometry, and it confirms the different polymorphisms at locus alpha(s1) casein.
Roncada
Paola
P
Laboratorio Analisi Proteomica, Istituto di Medicina Legale e Legislazione Veterinaria, Università degli Studi di Milano, Milano, Italy. roncadap@mailserver.unimi.it
Gaviraghi
Alessandro
A
Liberatori
Sabrina
S
Canas
Benito
B
Bini
Luca
L
Greppi
Gian Franco
GF
D000483:Alleles; D000818:Animals; D002364:Caseins / Q000737:chemistry* / Q000235:genetics; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006041:Goats; D007525:Isoelectric Focusing / Q000379:methods; D013058:Mass Spectrometry; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002364:Caseins; D008894:Milk Proteins