Toxicity of the explosives 2,4,6-trinitrotoluene, hexahydro-1,3,5-trinitro-1,3,5-triazine, and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine in sediments to Chironomus tentans and Hyalella azteca: low-dose hormesis and high-dose mortality.
The toxicity of the explosives 2,4,6-trinitrotoluene (TNT); hexahydro-1,3,5-trinitro-1,3,5-triazine (royal demolition explosive [RDX]); and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (high-melting explosive [HMX]), was evaluated in spiked sediment with two freshwater invertebrates. The midge Chironomus tentans and the amphipod Hyalella azteca demonstrated significant toxic effects after exposure to TNT and its degradation products, 1,3,5-trinitrobenzene (TNB) and 2,4-diamino-6-nitrotoluene (2,4-DANT). Significant reductions in survival of C. tentans exposed to TNT, TNB, and 2,4-DANT were observed at nominal sediment concentrations as low as 200 mg/kg. Hyalella azteca was more sensitive to TNT, TNB, and 2,4-DANT than the midge, where significant reductions in survival were observed at nominal concentrations of 50, 100, and 200 mg/kg, respectively. Survival of the midge and the amphipod was unaffected after exposure to RDX or HMX at the highest concentrations of 1,000 and 400 mg/kg, respectively. Growth of the midge, measured as total weight, was significantly reduced by 2,4-DANT. However, significantly increased growth was observed after exposure to sublethal concentrations of RDX and HMX. Although significant reductions in amphipod survival were observed at high concentrations of TNB, growth was significantly increased at sublethal concentrations. The results of the current investigation suggest that organisms exposed to explosives at contaminated sites may be affected at concentrations less than 25 mg/kg through hormetic growth enhancement and at higher concentrations through increased mortality.
US Army Corps of Engineers, Engineer Research and Development Center, CEERD-EP-R, Vicksburg, Misissippi 39180, USA. jeffrey.a.steevens@erdc.usace.army.mil
Jeffery A
JA
Steevens
B Maurice
BM
Duke
Guilherme R
GR
Lotufo
Todd S
TS
Bridges
D033304:Amphipoda / Q000187:drug effects / Q000254:growth & development; D000818:Animals; D001181:Arthropods / Q000187:drug effects / Q000254:growth & development*; D001392:Azocines / Q000633:toxicity*; D002683:Chironomidae / Q000187:drug effects / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D019015:Geologic Sediments / Q000737:chemistry; D006573:Heterocyclic Compounds, 1-Ring / Q000633:toxicity*; D036401:Toxicity Tests, Chronic; D014227:Triazines / Q000633:toxicity*; D014303:Trinitrotoluene / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D001392:Azocines; D006573:Heterocyclic Compounds, 1-Ring; D014227:Triazines; D014874:Water Pollutants, Chemical; D014303:Trinitrotoluene; C007950:octogen; C009160:cyclonite