Metabolic behavior of immobilized Candida guilliermondii cells during batch xylitol production from sugarcane bagasse acid hydrolyzate.
Candida guilliermondii cells, immobilized in Ca-alginate beads, were used for batch xylitol production from concentrated sugarcane bagasse hydrolyzate. Maximum xylitol concentration (20.6 g/L), volumetric productivity (0.43 g/L. h), and yield (0.47 g/g) obtained after 48 h of fermentation were higher than similar immobilized-cell systems but lower than free-cell cultivation systems. Substrates, products, and biomass concentrations were used in material balances to study the ways in which the different carbon sources were utilized by the yeast cells under microaerobic conditions. The fraction of xylose consumed to produce xylitol reached a maximum value (0.70) after glucose and oxygen depletion while alternative metabolic routes were favored by sub-optimal conditions.
Carvalho
Walter
W
Department of Biotechnology, Faculty of Chemical Engineering of Lorena, Rodovia Itajubá-Lorena, km 74.5, Lorena, S.P., Brazil, 12600-000.
Silva
Silvio S
SS
Converti
Attilio
A
Vitolo
Michele
M
D019342:Acetic Acid / Q000378:metabolism; D001089:Arabinose / Q000378:metabolism; D002175:Candida / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D008954:Models, Biological; D008956:Models, Chemical; D010100:Oxygen / Q000378:metabolism; D011134:Polysaccharides; D011492:Protein Hydrolysates; D012680:Sensitivity and Specificity; D013402:Sugar Alcohols / Q000378:metabolism; D014993:Xylitol / Q000096:biosynthesis*; D014994:Xylose / Q000378:metabolism*
D011134:Polysaccharides; D011492:Protein Hydrolysates; D013402:Sugar Alcohols; D002245:Carbon Dioxide; D000431:Ethanol; C007916:hemicellulose; D002482:Cellulose; C027433:bagasse; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D019342:Acetic Acid; D010100:Oxygen; D014993:Xylitol; C014999:arabitol