Uridine nucleotide-induced stimulation of gluconeogenesis in isolated rat proximal tubules.
Uridine nucleotides, released into the extracellular environment, influence a variety of metabolic and other cellular activities in a wide range of target tissues. Here we have studied the effects of uridine nucleotides on gluconeogenesis in isolated rat proximal tubules. Gluconeogenesis, from a range of precursors, was stimulated following exposure of isolated proximal tubules to either UTP or UTPgammaS, but not when exposed to other uridine-containing nucleotides. UTP- and UTPgammaS-induced gluconeogenesis was diminished in the presence of purinoceptor antagonists (e.g. suramin, PPADS) indicative of a role for P2Y(2)-like purinoceptors in these effects. Likewise, agents that interfere with either phospholipase C activation or intracellular Ca2+ mobilization decreased UTP- and UTPgammaS-induced stimulation of gluconeogenesis consistent with a role for these secondary messenger systems in the mechanism of action of extracellular UTP and UTPgammaS on proximal tubule metabolism.
Cellular Regulation and Signalling Group, School of Biological Sciences, University of Liverpool, UK.
Jun
J
Mo
Michael J
MJ
Fisher
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005943:Gluconeogenesis / Q000187:drug effects*; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D058914:Purinergic Antagonists; D051381:Rats; D017208:Rats, Wistar; D015290:Second Messenger Systems / Q000187:drug effects; D013268:Stimulation, Chemical; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors; D014500:Uracil Nucleotides / Q000494:pharmacology* / Q000502:physiology; D014544:Uridine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000502:physiology
D002121:Calcium Channel Blockers; D058914:Purinergic Antagonists; D014500:Uracil Nucleotides; D010738:Type C Phospholipases; D014544:Uridine Triphosphate