Influence of secondary colonizers and human plasma on the adherence of Porphyromonas gingivalis in vitro.
The influence of secondary colonizers (Fusobacterium nucleatum and Actinomyces naeslundii) and the effect of human plasma on the adherence of Porphyromonas gingivalis were investigated. Hydroxyapatite (HAP) discs coated with Streptococcus sanguis were immersed in a 3H-labeled bacterial cell suspension of F. nucleatum or A. naeslundii and then in a 14C-labeled P. gingivalis cell suspension. Bacterial cells on the discs were pyrolysed to quantify the radioisotopes released. The cell numbers of secondary colonizers on the discs increased with immersion time and this, in turn, resulted in significantly elevated adherence of P. gingivalis. These two secondary colonizers had very similar positive effects on the adherence of P. gingivalis. Human plasma significantly inhibited the adherence of P. gingivalis and secondary colonizers to S. sanguis-coated HAP discs. Adherence of P. gingivalis and A. naeslundii was strongly inhibited by plasma, while that of F. nucleatum was affected the least. Treatment with plasma, after immersion of streptococcal-coated discs in individual cell suspension of secondary colonizers, also reduced subsequent adherence of P. gingivalis. The rate of decrease was much smaller in F. nucleatum. These results indicate that both F. nucleatum and A. naeslundii enhance the adherence of P. gingivalis, and that the former may play a more important role in the establishment of P. gingivalis in dental plaque where plasma-derived components are present.
Sato
Masaru
M
Department of Oral Pathology, Asahi University School of Dentistry, Gifu, Japan. masaru44@beach.ocn.ne.jp
Nagayama
Motohiko
M
Yamaguchi
Ryozo
R
Fujiwara
Shuu
S
Takeuchi
Hiroshi
H
D000190:Actinomyces / Q000502:physiology*; D001422:Bacterial Adhesion; D001770:Blood Bactericidal Activity / Q000502:physiology; D002250:Carbon Radioisotopes; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000382:microbiology; D017886:Durapatite / Q000737:chemistry; D016967:Fusobacterium nucleatum / Q000502:physiology*; D006801:Humans; D007101:Immersion; D008297:Male; D016555:Matched-Pair Analysis; D010949:Plasma / Q000502:physiology*; D016966:Porphyromonas gingivalis / Q000502:physiology*; D019275:Radiopharmaceuticals; D013223:Statistics as Topic; D013298:Streptococcus sanguis / Q000502:physiology; D014316:Tritium
D002250:Carbon Radioisotopes; D019275:Radiopharmaceuticals; D014316:Tritium; D017886:Durapatite