Interactions between MC3T3-E1 cells and textured Ti6Al4V surfaces.
This paper presents the results of an experimental study of the interactions between MC3T3-E1 (mouse calvarian) cells and textured Ti6Al4V surfaces, including surfaces produced by laser microgrooving; blasting with alumina particles; and polishing. The multiscale interactions between MC3T3-E1 cells and these textured surfaces are studied using a combination of optical scanning transmission electron microscopy and atomic force microscopy. The potential cytotoxic effects of microchemistry on cell-surface interactions also are considered in studies of cell spreading and orientation over 9-day periods. These studies show that cells on microgrooved Ti6Al4V geometries that are 8 or 12 microm deep undergo contact guidance and limited cell spreading. Similar contact guidance is observed on the surfaces of diamond-polished surfaces on which nanoscale grooves are formed due to the scratching that occurs during polishing. In contrast, random cell orientations are observed on alumina-blasted Ti6Al4V surfaces. The possible effects of surface topography are discussed for scar-tissue formation and improved cell-surface integration.
Princeton Materials Institute, Princeton University, Princeton, New Jersey 08544, USA. soboyejo@princeton.edu
W O
WO
Soboyejo
B
B
Nemetski
S
S
Allameh
N
N
Marcantonio
C
C
Mercer
J
J
Ricci
D000497:Alloys / Q000627:therapeutic use; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002455:Cell Division; D002460:Cell Line; D004285:Dogs; D051379:Mice; D018625:Microscopy, Atomic Force; D008854:Microscopy, Electron; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology / Q000648:ultrastructure; D012886:Skull / Q000166:cytology*; D013499:Surface Properties; D023822:Tissue Engineering; D014025:Titanium / Q000627:therapeutic use*
D000497:Alloys; C031462:titanium alloy (TiAl6V4); D014025:Titanium