Export of acyl chains from plastids isolated from embryos of Brassica napus (L.).
We report the first measurements of the kinetics of transmembrane transport of acyl chains in plants. This was achieved by separating the period of in vitro synthesis of fatty acids from their export and by making use of acyl-CoA-binding protein (ACBP), which specifically binds long-chain acyl-CoAs. In the absence of added CoA but in the presence of ACBP, newly synthesised acyl chains accumulated as free fatty acids (FFAs) in plastids isolated from embryos of oilseed rape (Brassica napus L.). When CoA was added to plastids that had accumulated FFAs, the acyl chains were converted to acyl-CoAs that, in the presence of ACBP, were exported to the incubation medium. The rate of export was dependent on the CoA concentration and, at a saturating CoA concentration, was similar to the rate at which the fatty acids had been synthesised prior to CoA addition.
Johnson
Philip E
PE
Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK.
Rawsthorne
Stephen
S
Hills
Matthew J
MJ
D000214:Acyl Coenzyme A / Q000378:metabolism*; D000215:Acylation; D029688:Brassica napus / Q000502:physiology* / Q000648:ultrastructure; D002352:Carrier Proteins / Q000378:metabolism; D003065:Coenzyme A / Q000378:metabolism*; D018087:Plastids / Q000378:metabolism*; D012639:Seeds / Q000502:physiology
D000214:Acyl Coenzyme A; D002352:Carrier Proteins; D003065:Coenzyme A