Complete sequence and structure of ribosomal RNA gene of Heterosporis anguillarum.
The ribosomal RNA (rRNA) gene region of the microsporidium Heterosporis anguillarum has been examined. Complete DNA sequence data (4060 bp, GenBank Accession No. AF402839) of the rRNA gene of H. anguillarum are presented for the small subunit gene (SSU rRNA: 1359 bp), the internal transcribed spacer (ITS: 37 bp), and the large subunit gene (LSU rRNA: 2664 bp). The secondary structures of the H. anguillarum SSU and LSU rRNA genes are constructed and described. This is the first complete sequence of an rRNA gene published for a fish-infecting microsporidian species. In the phylogenetic analysis, the sequences, including partial SSU rRNA, ITS, and partial LSU rRNA sequences of the fish-infecting microsporidia, were aligned and analysed. The taxonomic position of H. anguillarum as suggested by Lom et al. (2000; Dis Aquat Org 43:225-231) is confirmed in this paper.
Tsai
Shu-Jen
SJ
Department of Entomology, National Taiwan University, Taipei.
Kou
Guang-Hsiung
GH
Lo
Chu-Fang
CF
Wang
Chung-Hsiung
CH
D000811:Anguilla / Q000469:parasitology*; D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000737:chemistry*; D005393:Fish Diseases / Q000175:diagnosis* / Q000469:parasitology; D016814:Microsporidia / Q000145:classification / Q000235:genetics*; D016881:Microsporidiosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics*
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal