Topographical analysis of Schizolobium parahyba chymotrypsin inhibitor (SPCI) by atomic force microscopy.
Atomic Force Microscopy (AFM) has been a useful tool for molecular surface analysis and to estimate topographical properties of proteins. Here we report a topographical study of a chymotrypsin inhibitor from Schizolobium parahyba seeds (SPCI) by AFM. The underlying structure of SPCI oligomers has been resolved in nanometer order resolution. SPCI oligomerize in hexagonal, ellipsoid, comet, pyramidal, and "Z" shaped. The hexagonal was the most observed oligomer shape.
Laboratório de Morfologia e Morfogênese, Departamento de Genética e Morfologia, Universidade de Brasília, DF, Brazil.
José Roberto S  A
JR
Leite
Luciano Paulino
LP
Silva
Clarice Cunha
CC
Taveira
Rozeni C  L
RC
Teles
Sonia M
SM
de Freitas
Ricardo Bentes
RB
Azevedo
D004591:Electrophoresis, Polyacrylamide Gel; D018625:Microscopy, Atomic Force / Q000379:methods*; D010940:Plant Proteins / Q000737:chemistry*; D011487:Protein Conformation
C096591:Chymotrypsin inhibitor protein, Schizolobium parahybum; D010940:Plant Proteins