Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method.
In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.
Masadome
Takashi
T
Department of Chemical Science and Engineering, Ariake National College of Technology, Omuta, Fukuoka 836-8585, Japan.
Asano
Yasukazu
Y
Imato
Toshihiko
T
Ohkubo
Satoshi
S
Tobita
Tatsuya
T
Tabei
Hisao
H
Iwasaki
Yuzuru
Y
Niwa
Osamu
O
Fushinuki
Yoshito
Y
D000906:Antibodies / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D002138:Calibration; D004867:Equipment Design; D006801:Humans; D011143:Polyvinyl Chloride / Q000737:chemistry; D012031:Refractometry; D012709:Serum Albumin / Q000276:immunology; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
D000906:Antibodies; D012709:Serum Albumin; D011143:Polyvinyl Chloride