Systemic vs. local cytokine and leukocyte responses to unilateral wrist flexion exercise.
We hypothesized that brief exercise of a small muscle group would lead to local rather than systemic alterations in cytokines, peripheral blood mononuclear cells, and mediators of angiogenesis. Fifteen men and eight women (age range 22-36 yr old) performed 10 min of unilateral wrist flexion exercise. Blood was sampled from venous catheters in the resting and exercising arm at baseline, at the end of exercise, and at 10, 30, 60, and 120 min after exercise. Lactate was significantly elevated in the exercising arm (+276 +/- 35%; P < 0.0005) with no change in the resting arm. In contrast, increases in both arms were observed for interleukin-6 (+139 +/- 51%; P < 0.0005), growth hormone (+1,104 +/- 284%; P < 0.003), natural killer cells (+81 +/- 9%; P < 0.0005), and lymphocytes expressing CD62L, CD11a, and CD54. There were no significant differences in these increases between the resting and exercising arm. Catecholamines increased in both arms [epinephrine peak increase, +226 +/- 36% (P < 0.001); norepinephrine peak increase, +90 +/- 15% (P < 0.01)]. Fibroblast growth factor-2 initially decreased with exercise in both arms, and this was followed by a rebound increase. Vascular endothelial growth factor demonstrated a small but significant increase in both arms (+124 +/- 31%; P < 0.05). Brief, low-intensity exercise leads to a systemic rather than local response of mediators that could be involved in inflammation, repair, or angiogenic adaptation to physical activity.
Nemet
Dan
D
Center for the Study of Health Effects of Exercise in Children, College of Medicine, University of California, Irvine 92868, California, USA.
Hong
Suzi
S
Mills
Paul J
PJ
Ziegler
Michael G
MG
Hill
Maryann
M
Cooper
Dan M
DM
D000328:Adult; D015815:Cell Adhesion Molecules / Q000378:metabolism; D016207:Cytokines / Q000097:blood*; D016228:Endothelial Growth Factors / Q000097:blood; D004837:Epinephrine / Q000097:blood; D015444:Exercise / Q000502:physiology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000097:blood; D006339:Heart Rate; D006400:Hematocrit; D019382:Human Growth Hormone / Q000097:blood; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D016131:Lymphocyte Subsets / Q000276:immunology*; D008222:Lymphokines / Q000097:blood; D008297:Male; D009638:Norepinephrine / Q000097:blood; D012737:Sex Factors; D012795:Sialoglycoproteins / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014955:Wrist Joint / Q000502:physiology
D015815:Cell Adhesion Molecules; D016207:Cytokines; D016228:Endothelial Growth Factors; C507763:IL1RN protein, human; D036341:Intercellular Signaling Peptides and Proteins; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D015850:Interleukin-6; D008222:Lymphokines; D012795:Sialoglycoproteins; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2; D019382:Human Growth Hormone; D019344:Lactic Acid; D009638:Norepinephrine; D004837:Epinephrine