In vitro selection and evaluation of RNA aptamers that recognize arginine-rich-motif model peptide on a quartz-crystal microbalance.
To study RNA-peptide interactions, we performed an in vitro selection of RNA on a simple alpha-helical peptide-immobilized quartz-crystal microbalance (QCM) and evaluated the association constants (10(7) M-1) of the selected RNA to the model peptide on the same QCM plate.
Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuda, Midori-ku, Yokohama 226-8501, Japan.
Shinobu
S
Fukusho
Hiroyuki
H
Furusawa
Yoshio
Y
Okahata
D020816:Amino Acid Motifs; D001120:Arginine / Q000737:chemistry*; D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry*; D011791:Quartz; D012313:RNA / Q000737:chemistry*
D010455:Peptides; D011791:Quartz; D012313:RNA; D001120:Arginine