Novel lipochitin oligosaccharide structures produced by Rhizobium etli KIM5s.
The novel lipochitin oligosaccharide (LCOs) structures produced by Rhizobium etli KIM5s were characterized using a nanoHPLC reverse-phase system coupled to an ion-trap mass spectrometer. This technique was shown to be more sensitive for structural elucidation of LCOs than previously used mass spectrometric methods. The structures of the LCOs of R. etli KIM5s, the majority containing six monosaccharide residues, differed from those synthesized by all other rhizobia analyzed to date. In addition, novel structures in which the chitin backbone was deacetylated at one or more GlcNAc moieties were found as minor compounds. The difference in host range of this strain compared to that of other known bean microsymbionts is discussed.
Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, NL-2333 AL, Leiden, The Netherlands.
Cristina
C
Pacios-Bras
Yuri E M
YE
van der Burgt
Andre M
AM
Deelder
Pablo
P
Vinuesa
Dietrich
D
Werner
Herman P
HP
Spaink
D000107:Acetylation; D002240:Carbohydrate Sequence; D002686:Chitin / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D000070116:Lotus / Q000382:microbiology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010641:Phenotype; D012231:Rhizobium / Q000737:chemistry* / Q000254:growth & development
D008070:Lipopolysaccharides; D002686:Chitin