Direct monitoring of DNA cleavages catalyzed by an ATP-dependent deoxyribonuclease on a 27 MHz quartz-crystal microbalance.
Kinetic studies of enzymatic DNA cleavage reactions (the enzyme binding, hydrolysis along DNA strands, and then release of the enzyme from the completely hydrolyzed ssDNA) were carried out on a 27 MHz quartz-crystal microbalance.
Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuda, Midori-ku, Yokohama 226-8501, CREST, JST (Japan Science and Technology Corporation), Japan.
Hisao
H
Matsuno
Hiroyuki
H
Furusawa
Yoshio
Y
Okahata
D000255:Adenosine Triphosphate; D004247:DNA / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008832:Microchemistry / Q000295:instrumentation*
D000255:Adenosine Triphosphate; D004247:DNA; D003851:Deoxyribonucleases