Hydroxychloroquine concentration-response relationships in patients with rheumatoid arthritis.
OBJECTIVE
A dose-response relationship for hydroxychloroquine (HCQ), in terms of the proportion of patients achieving the Paulus 20% criteria for improvement, had previously been observed in patients with rheumatoid arthritis (RA) receiving a 6-week loading regimen of 400, 800, or 1,200 mg HCQ daily. This present retrospective analysis was performed to investigate possible relationships between the blood HCQ and HCQ-metabolite concentrations and measures of efficacy and toxicity. In addition, we sought to ascertain whether further investigation of HCQ/HCQ-metabolite levels might lead to testing of one of these substances as a new antirheumatic drug.


METHODS
Patients with active RA (n = 212) began a 6-week, double-blind trial comparing 3 different doses of HCQ at 400, 800, or 1,200 mg/day, followed by 18 weeks of open-label HCQ treatment at 400 mg/day. Patients were repeatedly evaluated for treatment efficacy and toxicity. Blood samples were available from 123 patients for analysis of HCQ, desethylhydroxychloroquine (DHCQ), desethylchloroquine (DCQ), and bisdesethylchloroquine (BDCQ) levels using high-performance liquid chromatography. Achievement of the modified Paulus 20% improvement criteria for response in RA was used as the primary efficacy parameter. Spontaneously reported adverse events were categorized and analyzed as toxicity outcome variables. The relationship between response (efficacy and toxicity) and drug levels was evaluated using logistic regression analysis.


RESULTS
The subset of patients with blood concentration data was equivalent to the larger study population in all demographic and outcome characteristics. The mean HCQ, DHCQ, and DCQ elimination half-lives were 123, 161, and 180 hours, respectively. There was a positive correlation between the Paulus 20% improvement criteria response and blood DHCQ concentrations during weeks 1-6 (P < 0.001). A potential relationship between ocular adverse events and BDCQ levels was found (P = 0.036). Logistic regression analysis of adverse events data showed that adverse gastrointestinal events were associated with higher HCQ levels (P = 0.001-0.021) during weeks 1, 2, and 3.


CONCLUSIONS
There is a weak, but predictable, relationship between blood DHCQ concentrations and efficacy of treatment with HCQ. In addition, there is an association between gastrointestinal adverse events and elevated blood HCQ concentrations. Further investigation of these relationships is warranted to see if DHCQ may be introduced as a new antirheumatic drug.
University of Erlangen, Erlangen, Germany.
Tino
T
Munster
John P
JP
Gibbs
Danny
D
Shen
Bruce A
BA
Baethge
Gary R
GR
Botstein
Jacques
J
Caldwell
Fredrick
F
Dietz
Robert
R
Ettlinger
Harvey E
HE
Golden
Herbert
H
Lindsley
George E
GE
McLaughlin
Larry W
LW
Moreland
W Neal
WN
Roberts
Theodore W
TW
Rooney
Bruce
B
Rothschild
Marshall
M
Sack
Anthony I
AI
Sebba
Michael
M
Weisman
Kathryn E
KE
Welch
David
D
Yocum
Daniel E
DE
Furst
D000328:Adult; D018501:Antirheumatic Agents / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy*; D002738:Chloroquine / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000097:blood; D005128:Eye Diseases / Q000139:chemically induced; D005767:Gastrointestinal Diseases / Q000139:chemically induced; D006801:Humans; D006886:Hydroxychloroquine / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use*; D016015:Logistic Models; D015337:Multicenter Studies as Topic; D010084:Oxidation-Reduction; D016032:Randomized Controlled Trials as Topic; D012189:Retrospective Studies; D012720:Severity of Illness Index
D018501:Antirheumatic Agents; D006886:Hydroxychloroquine; C049283:cletoquine; D002738:Chloroquine; C033490:N,N-dideethylchloroquine; C007278:desethylchloroquine