[The significance of cyclin E expression and cyclin E-dependent kinase inhibitor in adult acute leukemia].
OBJECTIVE
To evaluate the function and clinical value of cyclin E and p27 in adult acute leukemia (AL).


METHODS
The protein expression of cyclin E, p27, p170 and cell cycle distribution was measured in the bone marrow samples of 60 adult patients with AL and 17 controls with flow cytometric analysis. The mRNA levels of cyclin E, p27, MRP in 46 cases of AL and 17 controls were examined with reverse transcription-polymerase chain reaction method.


RESULTS
The expression of cyclin E in AL patients was higher than that in controls (P < 0.01), so was the expression of p27 (P < 0.05). However in G(2)/M phase the expression rate of cyclin E in AL patients was not so apparently different with that in control (P < 0.05) so was that of p27 (P > 0.05). There was a positive correlation between the protein expression of cyclin E and p27. The remission rate (44.8%) in patients with over expression of cyclin E was lower than that (77.4%) in patients with normal expression of cyclin E (P < 0.01). The relapse rate (92.3%) in over expression group was higher than that (41.7%) in normal expression group (P = 0.003). No significant difference in remission rate or relapse rate was found between p27 over expression group and normal expression group (P > 0.50, P = 0.89).


CONCLUSIONS
Over expression of cyclin E may implicate occurrence and progression of AL. Over expression of cyclin E is an important influential factor for remission rate and relapse rate in AL patients.
Department of Hematology, The Second Hospital, Heibei Medical University, Shijiazhuang 050000, China.
Ying
Y
Wang
Shirong
S
Xu
Xiaonan
X
Guo
Fengru
F
Lin
Linna
L
Liu
Xingyan
X
Du
D018435:ATP Binding Cassette Transporter, Subfamily B; D000293:Adolescent; D000328:Adult; D018797:Cell Cycle Proteins / Q000235:genetics*; D019927:Cyclin E / Q000235:genetics*; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors*; D005260:Female; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D016196:S Phase; D025521:Tumor Suppressor Proteins / Q000235:genetics*
C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D018797:Cell Cycle Proteins; D019927:Cyclin E; D006023:Glycoproteins; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases