Developing site-specific immobilization strategies of peptides in a microarray.
In peptide-based microarrays, most existing methods do not allow for site-specific immobilization of peptides on the glass surface. We have developed two new approaches for site-specific immobilization of kinase substrates onto glass slides: (1) slides were functionalized with avidin for attachment of biotinylated peptides; and (2) slides were functionalized with thioester for attachment of N-terminally cysteine-containing peptides via a native chemical ligation reaction.
Lesaicherre
Marie Laure
ML
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
Uttamchandani
Mahesh
M
Chen
Grace Y J
GY
Yao
Shao Q
SQ
D000595:Amino Acid Sequence; D001360:Avidin / Q000737:chemistry; D019921:Biotinylation; D005898:Glass; D015394:Molecular Structure; D010455:Peptides / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D011487:Protein Conformation
D010455:Peptides; D001360:Avidin; D010770:Phosphotransferases