Ergot alkaloids produced by submerged cultures of Claviceps zizaniae.
Two ergopeptine alkaloids, alpha-ergocryptine (1) and its C(8) epimer alpha-ergocryptinine, have been isolated from the mycelium and fermentation broth of submerged cultures of Claviceps zizaniae CCM 8240. The structure of 1 was determined by HPLC/positive ion APCI MS and NMR analysis. Alkaloid concentrations of 10 microg/mL in 14-day-old fermentation broth and 1 mg/g of dry mycelium mass were found. These results are of considerable biotechnological interest since these were the only detectable alkaloids produced. Toxicity of naturally occurring sclerotia of C. zizaniae cannot be excluded.
Department of Analytical Chemistry, Faculty of Sciences, Charles University, Hlavova 2030, Prague 2, 12840, Czech Republic.
Michaela
M
Kantorová
Renata
R
Kolínská
Sylvie
S
Pazoutová
Ales
A
Honzátko
Vladimír
V
Havlícek
Miroslav
M
Flieger
D002170:Canada; D002851:Chromatography, High Pressure Liquid; D002967:Claviceps / Q000737:chemistry* / Q000378:metabolism*; D018153:Czech Republic; D004873:Ergolines / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D004876:Ergot Alkaloids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D005285:Fermentation; D013058:Mass Spectrometry; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D004873:Ergolines; D004876:Ergot Alkaloids; C100269:ergocryptine