Increased secretion of the pancreatic secretory trypsin inhibitor (PSTI-I, monitor peptide) during development of chronic pancreatitis in the WBN/Kob rat.
BACKGROUND
Recent genetic investigations into cationic trypsinogen and pancreatic secretory trypsin inhibitor (PSTI) led to the conclusion that mutations in either gene can contribute to the development of (hereditary) chronic pancreatitis. Since genetic animal models are not available yet, we have studied the Wistar-Bonn/Kobori (WBN/Kob) rat, a model for chronic pancreatitis (CP). To explore the possibility that PSTI may be secreted at lower levels or contain a mutation in the WBN/Kob rat, we investigated the masses of PSTI-I and -II and asked whether the ratio of PSTI/trypsinogen is decreased in animals with CP.


METHODS
We collected pancreatic juice from WBN/Kob and Wistar rats aged 6-36 weeks and measured PSTI-I (ELISA) and trypsin.


RESULTS
PSTI-I and -II were identified in Wistar and WBN/Kob rats by mass spectrometry and N-terminal sequencing. Using a newly developed PSTI-I ELISA, we can show that the PSTI-I/trypsinogen ratio is not decreased but rather increased in WBN/Kob rats compared to healthy Wistar rats. No evidence for a PSTI mutation was found.


CONCLUSIONS
Our data does not support the hypothesis that a dysbalance of PSTI/trypsinogen ratio is a causative factor for CP.
Graf
R
R
Pancreatitis Research Laboratory, Department of Visceral and Transplantation Surgery, University Hospital, Zürich, Switzerland. rgf@chi.usz.ch
Schiesser
M
M
Bimmler
D
D
D000818:Animals; D002908:Chronic Disease; D004195:Disease Models, Animal; D008297:Male; D010195:Pancreatitis / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000235:genetics / Q000378:metabolism*; D014362:Trypsinogen / Q000378:metabolism
D020033:Protein Isoforms; D012333:RNA, Messenger; D014359:Trypsin Inhibitor, Kazal Pancreatic; D014362:Trypsinogen