Size and complexity of the nuclear genome of the ectomycorrhizal fungus Paxillus involutus.
The basidiomycete Paxillus involutus is forming ectomycorrhizal symbiosis with a broad range of forest trees. Reassociation kinetics on P. involutus nuclear DNA indicated a haploid genome size of 23 Mb including 11% of repetitive DNA. A similar genome size (20 Mb) was estimated by genomic reconstruction analysis using three single copy genes. To assess the gene density in the P. involutus genome, a cosmid containing a 33-kb fragment of genomic DNA was sequenced and used to identify putative open reading frames (ORFs). Twelve potential ORFs were predicted, eight displayed significant sequence similarities to known proteins found in other organisms and notably, several homologues to the Podospora anserina vegetative incompatibility protein (HetE1) were found. By extrapolation, we estimate the total number of genes in the P. involutus haploid genome to approximately 7700.
Le Quéré
Antoine
A
Department of Microbial Ecology, Lund University, Ecology Building, Lund, Sweden. antoine.le_quere@mbioekol.lu.se
Johansson
Tomas
T
Tunlid
Anders
A
D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000235:genetics*; D003360:Cosmids / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000302:isolation & purification; D005800:Genes, Fungal; D014644:Genetic Variation; D016681:Genome, Fungal; D015698:Genomic Library; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid
D004271:DNA, Fungal