Strenuous exercise-induced change in redox state of human serum albumin during intensive kendo training.
A high-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) using an ion-exchange (DEAE-form) column shows three components: The principal component corresponds to human mercaptalbumin (HMA); the secondary to nonmercaptalbumin (HNA), having mixed disulfide with cystine (HNA[Cys]), or oxidized glutathione (HNA[Glut]); and the tertiary to HNA, oxidized more highly than mixed disulfide. The purpose of the present study is to clarify the effects of strenuous exercise load on HMA--><--HNA conversion (i.e., dynamic change in redox state) of HSA from elite kendo athletes (n=30; 20.0+/-1.1 years old). They participated in an intensive kendo training camp for 5 d. The mean value for the HMA fraction (f[HMA]) of kendo athletes after camp (62.8+/-2.4%) was significantly lower than before camp (71.9+/-3.7%) (p<0.0005). In contrast, the mean value for f(HNA-1) (i.e., f[HNA(Cys) and HNA(Glut)]) after camp (34.2+/-2.1%) was significantly higher than before camp (25.7+/-3.7%) (p<0.0005). These results suggested that strenuous physical exercise markedly increased the oxidized albumin level in extracellular fluids during the intensive training camp.
Imai
Hajime
H
Department of Health and Physical Education, Faculty of Education, Gifu University, Japan.
Hayashi
Tomoya
T
Negawa
Tsuneo
T
Nakamura
Koji
K
Tomida
Mihoko
M
Koda
Kunihide
K
Tajima
Tomio
T
Koda
Yasuko
Y
Suda
Kazuhiro
K
Era
Seiichi
S
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D008297:Male; D016139:Martial Arts / Q000502:physiology*; D010084:Oxidation-Reduction; D005082:Physical Exertion / Q000502:physiology*; D012709:Serum Albumin / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism
D012709:Serum Albumin; D013438:Sulfhydryl Compounds