Streptococcus mutans binding to solid phase dextran mediated by the glucan-binding protein C.
Streptococcus mutans GbpC is a wall-anchored surface protein which is involved in dextran-dependent aggregation. The GbpC phenotype is observed only in cells grown under stress conditions. In order to detect the GbpC protein of S. mutans, we isolated the wall fraction following digestion of the cell wall of this organism by N-acetylmuramidase, and detected the GbpC protein from S. mutans cells by western analysis with anti-GbpC serum. Interestingly, S. mutans cells exhibiting the negative dextran(alpha-1,6 glucan)-dependent aggregation (ddag) phenotype expressed the protein and could bind to immobilized dextran.
Department of Biochemistry, Tokyo Dental College, Masago 1-chome, Mihama-ku, Chiba City, Japan.
Y
Y
Sato
H
H
Senpuku
K
K
Okamoto
N
N
Hanada
H
H
Kizaki
D001422:Bacterial Adhesion / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry; D003911:Dextrans / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D037102:Lectins; D013295:Streptococcus mutans / Q000737:chemistry* / Q000502:physiology
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003911:Dextrans; D037102:Lectins; C037937:glucan-binding proteins