Cloning and expression of human beta-defensin-2 gene in Escherichia coli.
Human beta-defensin-2 (hBD-2), a small cationic peptide, exhibits a broad range of antimicrobial activity. It has been found to play important roles in innate and adaptive immune responses against microbial invasion. For the purposes of this study, hBD-2 gene was cloned from the lesions of human condyloma acuminatum. An expression vector was constructed and transformed into E. coli. hBD-2 was expressed as a fusion protein in both the soluble and insoluble forms, which was further confirmed by western blotting analysis.
The Central Laboratory of Sir Run Run Shaw Hospital, School of Medical Science, Zhejiang University, Hangzhou 310027, The People's Republic of China.
Xiangming
X
Fang
Li
L
Peng
Zhinan
Z
Xu
Jinmin
J
Wu
Peilin
P
Cen
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000378:metabolism*; D015153:Blotting, Western; D003001:Cloning, Molecular; D003218:Condylomata Acuminata / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023083:beta-Defensins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; C106641:DEFB4A protein, human; D017931:DNA Primers; D011993:Recombinant Fusion Proteins; D023083:beta-Defensins