Influence of pH on the xylose reductase activity of Candida guilliermondii during fed-batch xylitol bioproduction.
The influence of pH on the xylose reductase (XR) activity was studied. The fed-batch fermentation using exponential feeding rate was employed to produce xylitol. The feeding started when the xylose concentration in the fermenter reached 50 g/l and the pH was adjusted to 2.5, 4.0 or 6.0. The best results for XR activity (0.567 U/mg(protein)) and xylitol volumetric productivity (1.06 g/l.h) were achieved with pH 6.0.
Dept. of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo - Av. Lineu Prestes, 580 bl.16. 05508-900, São Paulo, SP, Brasil.
Denise Celeste
DC
Godoy De Andrade Rodrigues
Silvio Silvério
SS
Da Silva
Michele
M
Vitolo
D000449:Aldehyde Reductase / Q000378:metabolism*; D002175:Candida / Q000201:enzymology* / Q000254:growth & development*; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D014993:Xylitol / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
D003470:Culture Media; D014994:Xylose; D000449:Aldehyde Reductase; D014993:Xylitol