Adsorption on Cellulose of the Schizophyllum commune Cellobiose Dehydrogenase.
Equilibrium of adsorption of cellobiose dehydrogenase (CDH) of Schizophyllum commune on cotton cellulose, microcrystalline cellulose, and phosphate-swollen cellulose was reached within 4 min. The binding isotherms of CDH adsorbed on the above substrates were a straight line in Scatchard plot analysis, indicating that the binding fitted to the one-binding-site model. It was found that CDH adsorption onto microcrystalline cellulose was strongly affected by temperature, pH, NaCl, and ethylene glycol, respectively.
The State Key Lab of Microbial Technology, Shandong University, Jinan 250100, China. gaopj@life.sdu.edu.cn
Jing
J
Fang
Pei-Ji
PJ
Gao