PCR-cloning of cadmium-inducible peptides in the barnacle, Megabalanus volcano.
A 340 bp DNA fragment was amplified from barnacle (Megabalanus volcano) cDNA by polymerase chain reaction using primers designed based on the amino acid sequences of barnacle cadmium-inducible peptides CdlP1 and CdlP2. The whole sequence was determined by rapid amplification of cDNA ends method. The cDNA contained an open reading frame encoding 71 amino acid residues and the sequences for CdlP1 and CdlP2 were found to be located in the center of this coding region. Although CdlP1 and CdlP2 had been detected only in the cadmium-exposed barnacles, their mRNA was present both in cadmium-exposed barnacles and in unexposed barnacles. These results suggest that posttranslational proteolytic processing may be induced in the presence of cadmium.
Marine Biotechnology Institute, Shimizu Laboratories, 1900 Sodeshi, Shimizu, Shizuoka 424-0037, Japan. tougi001@smile2.sekisui.co.jp
Akiko
A
Togi
Kei
K
Kamino
Yoshikazu
Y
Shizuri
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002104:Cadmium / Q000494:pharmacology*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D001468:Thoracica / Q000187:drug effects* / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects*
D018076:DNA, Complementary; D010455:Peptides; D012333:RNA, Messenger; D002104:Cadmium