Chronological changes in pyridoxine-5'-phosphate oxidase immunoreactivity in the seizure-sensitive gerbil hippocampus.
To identify the roles of pyridoxine-5'-phosphate (PNP) oxidase in epileptogenesis and the recovery mechanisms in spontaneous seizure, a chronological and comparative analysis of PNP oxidase expression was conducted. PNP oxidase immunoreactivity in a preseizure group of seizure-sensitive (SS) gerbils was detected more strongly than that in a seizure-resistant (SR) group. The density of PNP oxidase immunoreactivity in a 30 min postictal group was significantly lower than that in a preseizure group. In a 12 hr postictal group, PNP oxidase immunodensity had recovered to a preseizure level. The overexpression of PNP oxidase in the hippocampus of preseizure SS gerbils suggests that PNP or pyridoxal 5'-phosphate plays an important role in the modulation of glutamic acid decarboxylase activity and gamma-aminobutyric acid reuptake as mediated by membrane transporter via neurons. In addition, this change in the PNP oxidase immunoreactivity following seizure may be a compensatory response designed to reduce epileptic activity in this animal.
Kang
Tae-Cheon
TC
Department of Anatomy, College of Medicine, Hallym University, Chunchon, South Korea. tckang@hallym.ac.kr
Park
Seung-Kook
SK
Hwang
In Koo
IK
An
Sung-Jin
SJ
Bahn
Jae Hoon
JH
Kim
A Yeon
AY
Choi
Soo Young
SY
Kwon
Oh-Shin
OS
Baek
Nam-In
NI
Lee
Hyeon Yong
HY
Won
Moo Ho
MH
D000818:Animals; D015153:Blotting, Western; D005849:Gerbillinae; D006624:Hippocampus / Q000201:enzymology*; D007150:Immunohistochemistry; D011734:Pyridoxaminephosphate Oxidase / Q000378:metabolism*; D012640:Seizures / Q000201:enzymology*; D013997:Time Factors
D011734:Pyridoxaminephosphate Oxidase