Production of laccase by a newly isolated strain of Trametes modesta.
The effects of the carbon and nitrogen sources, initial pH and incubation temperature on laccase production by Trametes modesta were evaluated using the one-factor-at-a-time method. The final optimisation was done using a central composite design resulting in a four-fold increase of the laccase activity to 178 nkat ml(-1). Response-surface analysis showed that 7.34 g l(-1) wheat bran, 0.87 g l(-1) glucose, 2.9 g l(-1) yeast extract, 0.25 g l(-1) ammonium chloride, an initial pH of 6.95 and an incubation temperature of 30.26 degrees C were the optimal conditions for laccase production. Laccase produced by T. modesta was fully active at pH 4 and at 50 degrees C. The laccase was very stable at pH 4.5 and at 40 degrees C but half-lives decreased to 120 and 125 min at higher temperature (60 degrees C) and lower pH (pH 3).
Institute of Biotechnology, Graz University of Technology, Austria.
G S
GS
Nyanhongo
J
J
Gomes
G
G
Gübitz
R
R
Zvauya
J S
JS
Read
W
W
Steiner
D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000737:chemistry; D005658:Fungi / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D042845:Laccase; D008962:Models, Theoretical; D009584:Nitrogen / Q000737:chemistry; D010088:Oxidoreductases / Q000096:biosynthesis*; D013696:Temperature; D013997:Time Factors
D002244:Carbon; D010088:Oxidoreductases; D042845:Laccase; D009584:Nitrogen