Evaluation of cytokine messenger RNA expression in peripheral blood mononuclear cells from dogs with canine demodicosis.
Using RT-PCR and semi-quantitative PCR, mRNA expression for canine interferon (IFN)-gamma, interleukin (IL)-2, IL-4, IL-5, IL-10, tumor necrosis factor (TNF)-alpha and transforming growth factor (TGF)-beta in peripheral blood mononuclear cells (PBMCs) was examined in dogs with or without demodicosis. mRNA expression for IFN-gamma as well as TNF-alpha in dogs with demodicosis (localized (LD) and generalized (GD)) was slightly lower than those in dogs without demodicosis (healthy controls). Expression of IL-5 mRNA in dogs with demodicosis was higher than that in control dogs, but there were no significant differences in IL-4 and IL-10 mRNA expression levels among the three groups. On the other hand, expression levels of TGF-beta mRNA in dogs with GD were higher than those in control dogs and dogs with LD. The expression levels of IL-5 and TGF-beta mRNA decreased in all three dogs with GD which showed resolution of the clinical signs. Taken together, these results suggest that the Th2-like response in PBMCs from dogs with demodicosis is up-regulated, and that subsequent increased expression of IL-5 and TGF-beta mRNA in dogs with GD is reversible after treatment. Therefore, these cytokines, particularly IL-5, might be a useful clinical index of the clinical course in demodicosis. Also, increased TGF-beta mRNA expression might be a key factor for revealing the difference in the mechanism of onset between LD and GD.
Tani
Kenji
K
Department of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University, Japan.
Morimoto
Masahiro
M
Hayashi
Toshiharu
T
Inokuma
Hisashi
H
Ohnishi
Takafumi
T
Hayashiya
Sanae
S
Nomura
Tadayoshi
T
Une
Satoshi
S
Nakaichi
Munekazu
M
Taura
Yasuho
Y
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D004283:Dog Diseases / Q000097:blood* / Q000276:immunology / Q000469:parasitology; D004285:Dogs; D005260:Female; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008924:Mite Infestations / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D012333:RNA, Messenger / Q000097:blood* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012876:Skin Diseases, Parasitic / Q000097:blood / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D018709:Statistics, Nonparametric
D016207:Cytokines; D012333:RNA, Messenger