High-throughput liquid chromatography ultraviolet/mass spectrometric analysis of combinatorial libraries using an eight-channel multiplexed electrospray time-of-flight mass spectrometer.
We report a high-throughput liquid chromatography/mass spectrometry (LC/MS) protocol for analyzing large combinatorial libraries using an eight-channel parallel LC/UV/MS (MUX-LCT) system. System configuration, linear response range in UV absorbance, LC column selection, and flow rate were optimized for 24 h/7 day unattended operations. Combinatorial libraries were analyzed on this system at a rate of 3200 compounds per day for a 3.5 min cycle time per injection. This parallel system is compared with a single-channel system in terms of performance and operation.
ChemRx Division, Discovery Partners International, 385 Oyster Point Blvd. Suite #1, South San Francisco, CA 94080, USA. fang@chemrx.com
Liling
L
Fang
Jason
J
Cournoyer
Michael
M
Demee
Jiang
J
Zhao
Duayne
D
Tokushige
Bing
B
Yan