Recombinant human parvovirus B19 vectors.
In an attempt to exploit the remarkable tissue-tropism of the human parvovirus B19 to target human hematopoietic cells of the erythroid lineage, recombinant human adeno-associated virus 2 genomes were encapsidated in parvovirus B19 capsids. Although efficient transduction of primary human hematopoietic cells in the erythroid lineage occurred, a low-level of transgene expression in non-erythroid cells was also detected. These studies suggest that cell surface expression of P antigen, the primary receptor for parvovirus B19, is necessary but not sufficient for parvovirus B19 vector-mediated transduction of human hematopoietic cells. These studies also suggest the existence of a putative cell surface co-receptor, which is required for successful infection of human hematopoietic cells by parvovirus B19.
Department of Microbiology & Immunology, Walther Oncology Center, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Kirsten A
KA
Weigel-Kelley
Arun
A
Srivastava
D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D000229:Dependovirus / Q000235:genetics; D015672:Erythroid Precursor Cells; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D016732:Parvovirus B19, Human / Q000235:genetics*; D011991:Receptors, Virus / Q000235:genetics; D014162:Transfection
D004274:DNA, Recombinant; D004279:DNA, Viral; D011991:Receptors, Virus; C101479:parvovirus B19 receptor