Expression and evolution studies of ets genes in a primitive coelomate, the polychaete annelid, Hediste (Nereis) diversicolor.
The Ets family includes numerous proteins with a highly conserved DNA-binding domain of 85 amino acids named the ETS domain. Phylogenetic analyses from ETS domains revealed that this family could be divided into 13 groups, among them are ETS and ERG. The ets genes are present in the Metazoan kingdom and we have previously characterized the Nd ets and Nd erg genes in the polychaete annelid Hediste diversicolor. Here, we isolated a fragment encoding the ETS domain from Nd Ets, by genomic library screening. By Northern blot analysis, we showed that this gene was transcribed as one major mRNA of 2.6 kb and one minor mRNA of 3.2 kb. By in situ hybridization, we observed that Nd ets was expressed in the intestine and oocytes and that Nd erg was expressed in cellular clumps present in the coelomic cavity, in an area of proliferating cells situated between the last metamere and the pygidium. Finally, we showed that Nd erg shared the expression pattern of Nd ets in oocytes. Molecular modeling studies have revealed that the spatial structure of ETS domain of Nd Ets and Nd Erg was conserved, in comparison to the murine Ets-1 and human Fli-1 proteins, respectively.
Laboratoire ELICO (Ecosystèmes Littoraux et Côtiers), groupe d'Ecologie Moléculaire, CNRS UPRES-A 8013, Bât. SN3, U.S.T.L., 59655 d'Ascq Cedex, Villeneuve, France.
Béatrice
B
Bocquet-Muchembled
Régine
R
Leroux
Anne
A
Chotteau-Lelièvre
Gérard
G
Vergoten
François
F
Fontaine
D000595:Amino Acid Sequence; D000818:Animals; D000845:Annelida / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D019143:Evolution, Molecular; D018260:Gelsolin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008840:Microfilament Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D015534:Trans-Activators; D014157:Transcription Factors / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*
C494041:ETS1 protein, human; C494042:Ets1 protein, mouse; C098179:FLII protein, human; D018260:Gelsolin; D008840:Microfilament Proteins; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D015534:Trans-Activators; D014157:Transcription Factors