Metabolic engineering of Lactococcus lactis: the impact of genomics and metabolic modelling.
Lactic acid bacteria display a relatively simple and well described metabolism where the sugar source is converted mainly to lactic acid. Here we will shortly describe metabolic engineering strategies that led to the efficient re-routing of the lactococcal pyruvate metabolism to end-products other than lactic acid, including diacetyl and alanine. Moreover, we will review current metabolic engineering approaches that aim at increasing the flux through complex biosynthetic pathways, leading to exopolysaccharides and folic acid. Finally, the (future) impact of the developments in the area of genomics and corresponding high-throughput technologies will be discussed.
Kleerebezem
Michiel
M
Department of Flavour, Nutrition and Natural Ingredients, Wageningen Centre for Food Sciences, NIZO Food Research, P.O. Box 20, 6710 BA Ede, The Netherlands. kleerebe@nizo.nl
Boels
Ingeborg C
IC
Groot
Masja Nierop
MN
Mierau
Igor
I
Sybesma
Wilbert
W
Hugenholtz
Jeroen
J
D005285:Fermentation; D005492:Folic Acid / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods*; D023281:Genomics; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D008956:Models, Chemical; D008957:Models, Genetic; D008958:Models, Molecular; D011135:Polysaccharides, Bacterial / Q000235:genetics / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism*
D011135:Polysaccharides, Bacterial; D019289:Pyruvic Acid; D005492:Folic Acid