Reactions of methane monooxygenase intermediate Q with derivatized methanes.
The reactivity of intermediate Q of soluble methane monooxygenase from Methylococcus capsulatus (Bath) with a series of derivatized methanes of general formula CH3-R, where R = CN, NO2, and OH, and their deuterated analogues is described. Despite relatively slow reactions, significant kinetic isotope effects, KIEs, are observed in reactions with acetonitrile and nitromethane; however, none is obtained with methanol. In addition, evidence of a substrate-binding step that occurs prior to substrate oxidation is observed.
Ambundo
Edna A
EA
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Friesner
Richard A
RA
Lippard
Stephen J
SJ
D007700:Kinetics; D008697:Methane / Q000031:analogs & derivatives* / Q000378:metabolism; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000737:chemistry* / Q000378:metabolism
D010105:Oxygenases; C021555:methane monooxygenase; D008697:Methane