The tandem repeated irritation test: a new method to assess prevention of irritant combination damage to the skin.
The effect of a protective cream was tested in a new tandem repeated irritation test with tandem application of 0.5% sodium lauryl sulphate (SLS) and undiluted toluene. The irritants were applied twice daily for 30 min to the ventral forearms of 20 volunteers. Irritant cutaneous reactions were quantified by a visual score, transepidermal water loss, chromametry and skin capacitance. Concurrent application of SLS/toluene induced stronger reactions than those caused by twice daily application of each irritant on its own. A protective effect of the protective cream was obtained against all treatment combinations and was significant for SLS/SLS (p < or = 0.01) and SLS/ toluene (p < or = 0.05). Our results indicate that the tandem repetitive irritation test has great potential in the evaluation of skin care products to prevent irritant contact dermatitis.
Department of Dermatology, Friedrich-Schiller-University, Jena, Germany.
Walter
W
Wigger-Alberti
Julia
J
Spoo
Sibylle
S
Schliemann-Willers
Andreas
A
Klotz
Peter
P
Elsner
D000293:Adolescent; D000328:Adult; D001557:Benzenesulfonates / Q000627:therapeutic use; D003124:Colorimetry; D017453:Dermatitis, Irritant / Q000175:diagnosis / Q000209:etiology / Q000473:pathology* / Q000517:prevention & control; D004338:Drug Combinations; D005260:Female; D005542:Forearm; D005712:Galvanic Skin Response; D006801:Humans; D007509:Irritants / Q000008:administration & dosage / Q000009:adverse effects*; D007809:Lanolin / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000187:drug effects / Q000473:pathology; D012882:Skin Tests / Q000379:methods; D012967:Sodium Dodecyl Sulfate / Q000008:administration & dosage / Q000009:adverse effects*; D013228:Stearates / Q000627:therapeutic use; D014050:Toluene / Q000008:administration & dosage / Q000009:adverse effects; D014870:Water Loss, Insensible
D001557:Benzenesulfonates; D004338:Drug Combinations; D007509:Irritants; D013228:Stearates; C080467:Stokoderm Salbe; D012967:Sodium Dodecyl Sulfate; D014050:Toluene; D007809:Lanolin