[Study on the effect of mouse pregnancy immunological tolerance induced by oral antigen administration].
OBJECTIVE
To observe the effect of mouse pregnancy immunological tolerance induced by oral administration of antigens and investigate oral tolerance (OT) therapy of unexplained recurrent spontaneous abortion.


METHODS
The mouse models of spontaneous abortion CBA/J x DBA/2 were established, then we administrated the models by oral proper dosage antigens ovum albumin (OVA), trophoblast menbrane antigens trophoblast membrane antigen (TMA)1, TMA2 and observed the changes of embryo loss rate (ELR) as compared with the control groups: group without immunization, group of active immunization by injecting intraperitoneally spleenocytes and blank control by feeding normal saline (NS).


RESULTS
(1) The results showed that the ELR of group without immunization, group of active immunization and control blank were 29%, 8% and 28%, respectively. (2) Expect the groups of low dosage single-fed, the ELR can be decreased significantly by oral administration of suitable dosage of antigen OVA and TMA2 as compared with group without immunization and blank control (P < 0.05) and no changes was found as compared with group of active immunization (P > 0.05), the ELR of group OVA 2 mg x 5-fed (4%) and TMA2 2 mg x 5-fed (3%) was the lowest of all. (3) The ELR had not been decreased after administration of TMA1 groups as compared with group without immunization and group blank (P > 0.05).


CONCLUSIONS
The results suggested that pregnancy immunological tolerance can be induced efficiently and the ELR can be decreased significantly by oral administration of proper dosage antigens of OVA and TMA2.
Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Aimin
A
Zhao
Qide
Q
Lin
Shimin
S
Bao
D000026:Abortion, Habitual / Q000188:drug therapy / Q000276:immunology; D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects / Q000276:immunology*; D014327:Trophoblasts / Q000276:immunology
D000941:Antigens; D010047:Ovalbumin