The tomato RNA-directed RNA polymerase has no effect on gene silencing by RNA interference in transgenic mice.
Double-stranded RNA (dsRNA) has been shown to interfere with the function of specific genes in various invertebrate species. The application of dsRNA interference (RNAi) in vertebrates (zebrafish and mouse) is still limited to embryos and it is not clear whether the method is generally applicable. Using a transgenic mouse model we investigated whether a stably inherited dsRNA introduced as a transgene can interfere with the expression of a specific target gene in erythroid tissue during development. In our globin gene system we do not observe any specific RNA interference. We, therefore, also introduced another gene that may be involved in a mechanism of post transcriptional gene silencing (PTGS), namely RNA-dependent RNA polymerase (RdRP) that was proposed to be involved in producing RNAs that trigger PTGS in plants. However, even though the tomato RdRP is catalytically active in erythroid tissue, no RNAi was observed.
Department of Cell Biology and Genetics, Faculty of Medicine, Erasmus University, Rotterdam, The Netherlands.
Ton
T
de Wit
Frank
F
Grosveld
Dubravka
D
Drabek
D000818:Animals; D005914:Globins / Q000235:genetics; D006801:Humans; D018551:Solanum lycopersicum / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics* / Q000378:metabolism
D012330:RNA, Double-Stranded; D005914:Globins; D012324:RNA-Dependent RNA Polymerase