[Expression of insulin-like growth factor-I in bovine trabecular meshwork cells in vitro].
OBJECTIVE
To determine whether cultured bovine trabecular meshwork cells in vitro would express insulin-like growth factor-I (IGF-I).


METHODS
Newborn bovine trabecular meshwork cells of the third passage were cultured in vitro. The whole RNA from 10(6) cells was extracted with Trizol reagent, and specific oligonucleotide primer pair and reverse transcription polymerase chain reaction (RT-PCR) were used for detection of IGF-I messenger RNA. Immunohistochemical stain was used to detect IGF-I protein.


RESULTS
Cultured cells had the specific characteristics of bovine trabecular meshwork cells. A single RT-PCR amplified product whose sequence was homologous to the known sequence was obtained. IGF-I immunostain was positive.


CONCLUSIONS
Trabecular meshwork cells may produce IGF-I. This finding strongly supports the possibility that IGF-I may affect trabecular meshwork cells through paracrine and autocrine mechanisms.
Department of Ophthalmology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ytsao@sohu.com
Yang
Y
Cao
Jihua
J
Dong
Houren
H
Wei
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014129:Trabecular Meshwork / Q000166:cytology / Q000378:metabolism*
D007334:Insulin-Like Growth Factor I