The effect of hydration on the stiffness of intervertebral discs in an ovine model.
OBJECTIVE
To determine the hydration-over-time behaviour of ovine intervertebral discs and intact joints in a saline bath at body temperature and the effect this has on their stiffness compared to air at ambient temperature.


METHODS
The hydration-over-time behaviour and stiffness of the ovine functional spinal unit and disc were quantified.


BACKGROUND
The fluid content of an intervertebral disc is not constant but varies with external load and load history. The stiffness of ovine functional spinal units in a hydrated environment and how this compares to testing in air have not been quantified.


METHODS
Intervertebral discs and functional spinal units were weighed and soaked in a saline water bath at 37 degrees C and reweighed each hour for 6 h. They were then allowed to stand in air at room temperature while the time to return to initial weight was recorded. Functional spinal units were randomly assigned to two groups. Axial compression, flexion, extension, lateral bending and axial torsion tests were performed on both the intact functional spinal unit and isolated disc. Group 1 was tested in air then in a saline water bath at 37 degrees C with the testing order reversed for Group 2.


RESULTS
Hydration of the disc reached a plateau after an average 3-4 h of soaking with the largest increase seen in the first hour. Four hours, standing in air at room temperature, was required to return specimens to their initial weight. The functional spinal unit stiffness was significantly lower for those specimens tested in the bath compared to air.


CONCLUSIONS
Ovine intervertebral discs show similar hydration-over-time behaviour when compared to human discs. Stiffnesses in different modes of loading were significantly different when tested in a hydrated environment compared with the standard method of testing in air.


CONCLUSIONS
It has been shown that there are biomechanical and biochemical similarities between sheep and human intervertebral discs. Despite these similarities, no studies have looked at how ovine intervertebral discs behave over time in a hydrated environment. In humans, hydration levels are an important aspect of intervertebral disc degeneration. There is also a relationship between decreased hydration levels and increased stiffness. This study demonstrates the similarities between human and ovine hydration-over-time behaviour. The importance of intervertebral disc hydration and its effects on stiffness under different modes of loading were also demonstrated and have not been previously shown using the ovine model. In this context, the results from this study provide further support for the use of the ovine model.
Department of Orthopaedics, Division of Surgery, Repatriation General Hospital and School of Informatics and Engineering, Flinders University, Daws Road, Daw Park, South Australia 5041, Australia. john.costi@rgh.sa.gov.au
John J
JJ
Costi
Trevor C
TC
Hearn
Nicola L
NL
Fazzalari
D000704:Analysis of Variance; D000818:Animals; D001696:Biomechanical Phenomena; D001834:Body Water / Q000737:chemistry*; D003681:Dehydration; D066298:In Vitro Techniques; D007403:Intervertebral Disc / Q000737:chemistry* / Q000502:physiology*; D008159:Lumbar Vertebrae / Q000502:physiology; D012044:Regression Analysis; D012756:Sheep; D012965:Sodium Chloride; D013048:Specimen Handling / Q000379:methods; D018709:Statistics, Nonparametric
D012965:Sodium Chloride