Identification of a type III secretion system in uropathogenic Escherichia coli.
To determine virulence-related genes in uropathogenic Escherichia coli (UPEC) showing invasiveness to T-24 bladder cancer cells, genomic subtractive hybridization was performed between a highly invasive and a less invasive strain. Forty-nine DNA fragments were isolated from the invasive strain. One of them showed homology with Salmonella invA gene. By chromosomal walking of the strain, a type III secretion system that has been described in E. coli O157:H7 was identified on the genome of the invasive strains. Three strains out of 100 UPEC isolates had a type III secretion system inserted at 64 min of the chromosome, corresponding to E. coli K-12 MG1655. This finding suggested that the type III secretion system could play a part in uropathogenicity of UPEC.
Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba-shi, Ibaraki 305-8575, Japan. md995454f@md.tsukuba.ac.jp
Jun
J
Miyazaki
William
W
Ba-Thein
Toshio
T
Kumao
Hideyuki
H
Akaza
Hideo
H
Hayashi
D020130:Bacterial Chromatophores; D001426:Bacterial Proteins / Q000235:genetics; D012634:Bodily Secretions / Q000502:physiology*; D002675:Child, Preschool; D002874:Chromosome Mapping; D003556:Cystitis / Q000453:epidemiology / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D016680:Genome, Bacterial; D006801:Humans; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D015995:Prevalence; D011704:Pyelonephritis / Q000453:epidemiology / Q000382:microbiology; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms; D014552:Urinary Tract Infections / Q000453:epidemiology / Q000382:microbiology*; D014774:Virulence
D001426:Bacterial Proteins; C075518:invA protein, Bacteria