Coexpression of SCL and GATA3 in the V2 interneurons of the developing mouse spinal cord.
The differentiation of neural progenitors into the many classes of neurons that exist in the mature spinal cord is a process that relies heavily on the activation of precise combinations of transcription factors. Defining these transcription factor combinations is an important aspect of research in developmental neurobiology that promises to provide incredible insights into the structure, function, and pathology of the central nervous system. The present study aimed to investigate a possible role for the Stem Cell Leukemia (SCL) gene, a basic helix-loop-helix (bHLH) transcription factor gene, in the specification of a population of neural cells in the ventral neural tube. Section RNA in situ hybridisation revealed that SCL is transiently expressed within the V2 postmitotic domain of the developing mouse spinal cord between 10.5 and 13.5 days post coitum. Double-immunofluorescence experiments were subsequently carried out to directly compare the expression of SCL with other V2-specific markers at the cellular level. These experiments revealed that SCL is expressed in a medially restricted subpopulation of GATA-3 producing cells, suggesting a possible role for this factor in the differentiation of the GATA population of V2 interneurons.
Institute for Molecular Bioscience, incorporating the Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane, Australia.
Emma
E
Smith
Murray
M
Hargrave
Toshiya
T
Yamada
C Glenn
CG
Begley
Melissa Helen
MH
Little
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D005455:Fluorescent Antibody Technique; D050990:GATA3 Transcription Factor; D017403:In Situ Hybridization; D051379:Mice; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D012313:RNA / Q000378:metabolism; D013116:Spinal Cord / Q000196:embryology*; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D013997:Time Factors; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; D011518:Proto-Oncogene Proteins; D012313:RNA; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D015534:Trans-Activators; D014157:Transcription Factors; C494748:Gata3 protein, mouse; C489366:Tal1 protein, mouse