Interleukin-5 messenger RNA expression in peripheral blood mononuclear cells from patients with bronchial asthma and eosinophilia.
Interleukin-5 (IL-5) plays an important role in allergic diseases accompanied by eosinophilia. We examined IL-5 messenger RNA (mRNA) expression in peripheral blood mononuclear cells (PBMC) isolated from 13 patients with bronchial asthma, 1 patient with chronic eosinophilic pneumonia, 2 patients with idiopathic eosinophilia, and 5 control subjects. IL-5 mRNA was expressed in PBMC from 2 of 13 patients with asthma, 1 patient with chronic eosinophilic pneumonia, and 1 of 2 patients with idiopathic eosinophilia. IL-5 mRNA expression was not detected in PBMC from control subjects. PBMC from a patient with chronic eosinophilic pneumonia expressed IL-5 mRNA spontaneously. Prednisolone treatment decreased his clinical symptoms and IL-5 mRNA expression. IL-5 mRNA expression preceded revival of the disease. These observations show that IL-5 plays a role in the condition, accompanied by eosinophilia, and IL-5 mRNa examination in PBMC by means of reverse transcription-polymerase chain reaction may be useful to predict the activity of eosinophilia.
First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Takao
T
Ishizuka
Tamotsu
T
Ishizuka
Shigenori
S
Iwamae
Takeshi
T
Hisada
Yoshimichi
Y
Okayama
Hideo
H
Tsukagoshi
Kunio
K
Dobashi
Masatomo
M
Mori
D000293:Adolescent; D000328:Adult; D000368:Aged; D001249:Asthma / Q000235:genetics* / Q000503:physiopathology*; D002908:Chronic Disease; D004802:Eosinophilia / Q000235:genetics* / Q000503:physiopathology*; D005260:Female; D015870:Gene Expression / Q000235:genetics* / Q000502:physiology; D006801:Humans; D015848:Interleukin-5 / Q000032:analysis* / Q000235:genetics* / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015848:Interleukin-5; D012333:RNA, Messenger