[Effect of cilostazol on adhesion molecules of STZ-induced diabetic rats].
OBJECTIVE
To investigate the effect of cilostazol on the expression of CD(54), CD(106), CD(62P) in kidney, aorta, surface of platelet, and mononuclear cell of blood in STZ-induced diabetic rats.


METHODS
Rats were randomly divided into four groups: normal control group (n = 8), diabetes control group (n = 8), insulin group (n = 7 subcutaneous injection of insulin, 1 approximately 4 U * d(-1)), and cilostazol group (n = 7, gastric infusion of cilostazol, 18 mg * kg(-1) * d(-1)). Sciatic nerve conductive velocity and level of CD(54)/CD(62p) on the surface of mononuclear cell/platelet were examined by flow cytometry. Immunohistochemistry was used to examine the expression of CD(54) and/or CD(106) in kidney and aorta.


RESULTS
The sciatic nerve conductive velocity was 20.3 +/- 2.2 m * s(-1) and 28.9 +/- 7.9 m * s(-1) in diabetic rats and cilostazol group respectively (P < 0.05). The mononuclear cell surface expression of CDa-a(c)54 was 69.1 +/- 14.9% and 25.9 +/- 8.6% in diabetic group and cilostazol group respectively (P < 0.05). The expression of CD(54)/CD(106) in kidney and aorta was decreased. The expression of CD(62p) on the surface of platelets was 40.4 +/- 8.7% in diabetic group and 28.5 +/- 3.7% in cilostazol group (P > 0.05).


CONCLUSIONS
Cilostazol treatment delays the development of diabetic pathological change in kidney, aorta and sciatic nerve and decreases the expression of CD(54) and CD(106).
Wang
Zhe
Z
Department of Endocrinology, Shandong Provincial Hospital, Shandong University, Jinan, China.
Zhao
Jiajun
J
Gao
Ling
L
Zhang
Qunye
Q
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000187:drug effects*; D000077407:Cilostazol; D005434:Flow Cytometry; D006442:Glycated Hemoglobin / Q000187:drug effects / Q000378:metabolism; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D019007:P-Selectin / Q000096:biosynthesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000378:metabolism*; D013777:Tetrazoles / Q000494:pharmacology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology*
D015815:Cell Adhesion Molecules; D000077407:Cilostazol; D006442:Glycated Hemoglobin; D018799:Intercellular Adhesion Molecule-1; D019007:P-Selectin; D013311:Streptozocin; D013777:Tetrazoles; D019010:Vascular Cell Adhesion Molecule-1; D014665:Vasodilator Agents