Pharmacological characterization of beta-endorphin- and dynorphin A(1-17)-induced feeding using G-protein alpha-subunit antisense probes in rats.
Antisense (AS) oligodeoxynucleotides targeting G-protein alpha-subunits distinguish feeding responses of morphine and its metabolite, as well as nocturnal and deprivation-induced feeding. The present study examined whether feeding elicited by beta-endorphin (betaEND) or dynorphin A(1-17) was altered by ventricularly-applied G(i)alpha(1), G(i)alpha(2), G(i)alpha(3), G(s)alpha, G(o)alpha, G(q)alpha or G(x/z)alpha AS probes, or a nonsense (NS) control. The betaEND-induced feeding was reduced by the G(i)alpha(1) and G(x/z)alpha AS probes, and increased by G(i)alpha(2) or G(i)alpha(3) AS treatment. Dynorphin-induced feeding was attenuated by G(i)alpha(1) and G(o)alpha AS treatment. Yet, G(s)alpha or G(q)alpha AS and NS treatments failed to alter opioid agonist-induced feeding. These data provide initial characterization of potential effector signaling pathways mediating betaEND and dynorphin-induced feeding.
Department of Psychology and Neuropsychology Doctoral Sub-Program, Queens College, City University of New York, 65-30 Kissena Boulevard, Flushing, NY 11367, USA.
Robert M
RM
Silva
Henya C
HC
Grossman
Grace C
GC
Rossi
Gavril W
GW
Pasternak
Richard J
RJ
Bodnar
D000818:Animals; D004399:Dynorphins / Q000737:chemistry* / Q000378:metabolism*; D005247:Feeding Behavior / Q000187:drug effects; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008297:Male; D009294:Narcotics / Q000819:agonists; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D017434:Protein Structure, Tertiary; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D001615:beta-Endorphin / Q000378:metabolism*
D009294:Narcotics; D016376:Oligonucleotides, Antisense; D001615:beta-Endorphin; D004399:Dynorphins; D019204:GTP-Binding Proteins