Adenovirus replication-competent vectors (KD1, KD3) complement the cytotoxicity and transgene expression from replication-defective vectors (Ad-GFP, Ad-Luc).
The successful clinical application of adenovirus (Ad) in cancer control has been of limited success because of the current inability to infect the majority of cancer cells with a large amount of vector. In this study, we show that when human lung tumors growing in immunodeficient nude mice were coinfected with a replication-defective (RD) Ad vector expressing green fluorescent protein and a replication-competent (RC) Ad vector named KD3, KD3 enhanced the expression of green fluorescent protein throughout the tumor. Also, KD3 and another RC vector named KD1 complemented the expression of luciferase from a RD vector in a human liver tumor xenotransplant in nude mice. Altogether, these results suggest that the combination of a RD vector with a RC vector might be a more effective treatment for cancer than either vector alone due to more widespread dissemination of the virus.
Habib
Nagy A
NA
Department of Surgery, Faculty of Medicine, Imperial College, Hammersmith Hospital Campus, London, UK. nagy.habib@ic.ac.uk
Mitry
Ragai
R
Seth
Prem
P
Kuppuswamy
Mohan
M
Doronin
Konstantin
K
Toth
Karoly
K
Krajcsi
Peter
P
Tollefson
Ann E
AE
Wold
William S M
WS
D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D000818:Animals; D002470:Cell Survival / Q000235:genetics*; D003673:Defective Viruses / Q000235:genetics*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D019076:Transgenes; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D008156:Luciferases