Molecular characterisation of airport malaria: four cases in France during summer 1999.
Four airport malaria cases have been observed in the vicinity of the Roissy-Charles-de-Gaulle International Airport, Paris, France. These cases were geographically very close to each other and clustered in a short period of time during the summer of 1999. The phenotype and genotype of the Plasmodium falciparum isolates obtained from these patients were determined in order to know whether a single mosquito could have infected more than one subject. The genomic characterisation of isolates was performed using the polymorphic markers merozoite surface protein 1 (Msp 1) and merozoite surface protein 2 (Msp 2) genes, the kappa and omega repeats domains of cg2 and the dihydrofolate reductase (DHFR) genotypes. Results showed identical genotypes for isolates 1, 2 and 4 whereas the genotype of isolate 3 differed at one locus. The molecular analysis was consistent with the hypothesis that all patients could have been bitten by the same mosquito and that patient 3, may have received a different clone and an additional species. In vitro susceptibility data did not confirm or rule out this hypothesis because isolates had the same profile of susceptibility to the tested drugs.
Centre National de Référence pour la Chimiosensibilité du Paludisme, Assistance Publique-Hôpitaux de Paris, Laboratoire de Parasitologie, Hôpital Bichat-Claude Bernard, 46, Rue Henri Huchard, 75877 Paris, France. cnrcp.parasit1@bch.ap-hop-paris.fr
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Jafari
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Durand
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Lusina
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Le Bras
D000328:Adult; D000337:Aerospace Medicine; D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D000953:Antigens, Protozoan / Q000235:genetics; D000962:Antimalarials / Q000494:pharmacology; D003062:Codon / Q000235:genetics; D016054:DNA, Protozoan / Q000032:analysis; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology*; D008288:Malaria / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission; D016778:Malaria, Falciparum / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000235:genetics; D009784:Occupational Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D010297:Paris / Q000453:epidemiology; D010963:Plasmodium falciparum / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D010965:Plasmodium malariae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014195:Travel
D000953:Antigens, Protozoan; D000962:Antimalarials; C109422:CG2 protein, Plasmodium falciparum; D003062:Codon; D016054:DNA, Protozoan; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium; D013762:Tetrahydrofolate Dehydrogenase