Temporal and spatial expression of two isoforms of the Dutt1/Robo1 gene in mouse development.
The mammalian homologue of the Drosophila axonal guidance receptor roundabout is expressed in a wide range of tissues. Here we show that alternative splicing of the Dutt1/Robo1 gene results in two mRNA transcripts with different signal peptides, which are differentially expressed throughout mouse embryogenesis. Since mice with a targeted deletion in the Dutt1/Robo1 gene have abnormal lung pathology, immunohistochemistry was used to identify the cellular expression pattern of Dutt1/Robo1 during lung development. Dutt1/Robo1 expression was widespread and diffuse in the lung at embryonic day 17.5 but became increasingly localised to the bronchial epithelium in newborn and adult mice.
Department of Oncology, University of Cambridge, MRC Centre, Hills Road, CB2 2QH, Cambridge, UK. kjclark@mrc-lmb.ac.uk
Katherine
K
Clark
Ester
E
Hammond
Pamela
P
Rabbitts
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001980:Bronchi / Q000254:growth & development / Q000378:metabolism*; D004848:Epithelium / Q000254:growth & development / Q000378:metabolism; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D008168:Lung / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D021382:Protein Sorting Signals; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics*; D014018:Tissue Distribution; D000097905:Roundabout Proteins
D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins