In vitro release of cytotoxic nucleoside analogs from lactide-caprolactone and lactide-glycolide copolymers.
The aims of our study were to assess the release of cytotoxic nucleoside analogs 5-fluorouracil and 2-chloro-2'-deoxyadenosine from different lactide-glycolide or lactide-caprolactone biodegradable copolymers and the effects of sterilization on this release. The polymers were sterilized either with ethylene oxide at 37 degrees C, or with gamma radiation (15 kGy, 20 kGy, or 25 kGy). The kinetics of nucleoside release from the copolymers were measured over 50 days. Four copolymers exhibited relatively constant release of nucleosides in micromolar concentrations during the entire observation period. Sterilization with either ethylene oxide or gamma radiation only slightly influenced nucleoside release. Further development of these copolymers as an intracerebral nucleoside delivery system for local treatment of brain tumors is indicated.
Kryczka
Tomasz
T
Medical Research Centre Polish Academy of Sciences, Warsaw. tkryczka@mp.pl
Bero
Maciej
M
Kasperczyk
Janusz
J
Dobrzyński
Piotr
P
Marciniec
Barbara
B
Popielarz-Brzezińska
Maria
M
Grieb
Pawel
P
D000970:Antineoplastic Agents / Q000493:pharmacokinetics*; D001932:Brain Neoplasms / Q000188:drug therapy; D017338:Cladribine / Q000493:pharmacokinetics*; D005472:Fluorouracil / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D011091:Polyesters / Q000138:chemical synthesis / Q000493:pharmacokinetics*
D000970:Antineoplastic Agents; D011091:Polyesters; D017338:Cladribine; C062968:lactide-caprolactone copolymer; D005472:Fluorouracil