The hydroxyphenylpyruvate dioxygenase from Synechocystis sp. PCC 6803 is not required for plastoquinone biosynthesis.
The disruption of the Synechocystis open reading frame Deltaslr0090 encoding a gene with high homology to plant genes encoding 4-hydroxyphenylpyruvate dioxygenase results in an impairment of tocopherol biosynthesis without affecting levels of plastoquinone, carotenoids and chlorophyll as well as cell growth and photosynthesis. Our results indicate that in Synechocystis in contrast to the situation in higher plants the 4-hydroxyphenylpyruvate dioxygenase is not required for the synthesis of plastoquinone.
Dähnhardt
Dorothee
D
Institute of Botany, University of Kiel, Olshausenstrasse 40, D-24098, Kiel, Germany.
Falk
Jon
J
Appel
Jens
J
van der Kooij
Tom A W
TA
Schulz-Friedrich
Rüdiger
R
Krupinska
Karin
K
D015111:4-Hydroxyphenylpyruvate Dioxygenase / Q000235:genetics / Q000378:metabolism*; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D045342:Light-Harvesting Protein Complexes; D009154:Mutation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D010971:Plastoquinone / Q000032:analysis / Q000378:metabolism*; D024505:Tocopherols / Q000032:analysis; D010837:Vitamin K 1 / Q000032:analysis
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D010837:Vitamin K 1; D015111:4-Hydroxyphenylpyruvate Dioxygenase; D010971:Plastoquinone; D024505:Tocopherols