The effect of intra-articular methylprednisolone acetate and exercise on equine carpal subchondral and cancellous bone microhardness.
Dorsal carpal osteochondral injury is a major cause of lameness in horses undergoing high intensity training. Intra-articular corticosteroid treatment is used commonly to manage exercise-associated articular pain, but its use remains highly controversial in the equine athlete. This project, therefore, aimed to compare the mechanical properties of intra-articular MPA and diluent-treated middle carpal subchondral and cancellous bone in horses undergoing a short-term treadmill exercise programme. It was hypothesised that subchondral and cancellous bone mechanical properties are influenced by intra-articular administration of methylprednisolone acetate (MPA). Eight 2-year-old female horses had MPA or diluent administered into contralateral middle carpal joints at 14 day intervals, for a total of 4 treatments per horse. Horses underwent a standard treadmill exercise protocol until euthanasia (Day 70). Standard sites were located on the dorsal aspect of third, radial and intermediate carpal bones. Osteochondral samples from each test site were divided into subchondral bone and cancellous bone portions. These were dried, resin-embedded and gold-coated. Microhardness measurements were obtained at each test site. No significant effect of intra-articular treatment was detected. At each site, cancellous bone trabecular struts had an 18-19% higher microhardness value than the overlying subchondral bone. These findings indicate that intra-articular administration of MPA at this dose has no effect on subchondral or cancellous bone adaptation to short-term exercise and, therefore, on the propensity of carpal bones to injury. Further investigation into the calcified cartilage layer, effect of different corticosteroid preparations and diffusion of medication are required.
Centre for Equine Studies, Animal Health Trust, Newmarket, Suffolk, UK.
R C
RC
Murray
N
N
Znaor
K E
KE
Tanner
R M
RM
DeBowes
E M
EM
Gaughan
A E
AE
Goodship
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001696:Biomechanical Phenomena; D002348:Carpal Bones / Q000187:drug effects* / Q000502:physiology; D002350:Carpus, Animal / Q000187:drug effects* / Q000502:physiology; D005080:Exercise Test / Q000662:veterinary; D005260:Female; D006244:Hardness / Q000187:drug effects; D006736:Horses / Q000033:anatomy & histology* / Q000502:physiology; D007270:Injections, Intra-Articular / Q000662:veterinary; D008775:Methylprednisolone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000077555:Methylprednisolone Acetate; D010805:Physical Conditioning, Animal / Q000502:physiology*; D016896:Treatment Outcome
D000893:Anti-Inflammatory Agents; D000077555:Methylprednisolone Acetate; D008775:Methylprednisolone