High Expression and Purification of Recombinant Human Serum Albumin from Pichia pastoris.
Recombinant human serum albumin (rHSA) was produced in methylotrophic yeast Pichia pastoris. By optimization of expression, about 150 mg/L of rHSA was obtained from broth of Pichia pastoris GS115/HAS (his+Mut(S)) supernatant. The rHSA was isolated and purified by hollow-fiber ultrafiltration, Phenyl-Sepharose hydrophobic chromatography and antibody-immunoadsorbent chromatography. Finally, rHSA was purified to electrophoretic purity.
Shanghai Institute of Biochemistry, the Chinese Academy of Science, Shanghai 200031, China. zhongyi@sunm.shcnc.ac.cn
Rong-De
RD
Qiu
Shi-Yun
SY
Li
Jun-Gang
JG
Chen
Xiang-Fu
XF
Wu
Zhong-Yi
ZY
Yuan