Protein prosthesis: a semisynthetic enzyme with a beta-peptide reverse turn.
beta-Amino acids are incorporated into an enzyme by using the method of expressed protein ligation. In the resulting semisynthetic enzyme, an R-nipecotic acid-S-nipecotic acid module replaces Asn113 and Pro114 of ribonuclease A. The semisynthetic enzyme not only retains full catalytic activity but also gains conformational stability. Thus, structural elements can be replaced with foldameric equivalents to endow proteins with more desirable properties.
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA.
Ulrich
U
Arnold
Matthew P
MP
Hinderaker
Bradley L
BL
Nilsson
Bayard R
BR
Huck
Samuel H
SH
Gellman
Ronald T
RT
Raines
D002384:Catalysis; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012259:Ribonuclease, Pancreatic / Q000138:chemical synthesis* / Q000737:chemistry
D012259:Ribonuclease, Pancreatic