Serological evidence for recent exposure to Taenia solium in Venezuelan Amerindians.
This study examined the seroprevalence and serum antibody isotype profile for Taenia solium cysticercosis in an Amerindian community in the Amazonas state of Venezuela. An antigen-trapping enzyme-linked immunosorbent assay (Ag-ELISA) was used to detect viable cysticercosis. Indirect ELISA (Ab-ELISA) and enzyme-linked immunoelectrotransfer blot (EITB) was performed by using antigens prepared from T. solium metacestodes to detect anti-parasite antibodies. The Ag-ELISA and Ab-ELISAs revealed 64.7% and 79.0% seropositivity, respectively, in the Amerindian population. Immunoglobulin (Ig) M was the predominant antibody class, suggesting recent infection. In comparison sera from, clinically defined, hospital neurocysticercosis cases revealed only 27% seropositivity by Ag-ELISA, compared with 86-92% seropositivity by Ab-ELISA, and IgG4 was the predominant antibody subclass detected. The EITB antigen recognition patterns of the hospitalized patients were very similar to that of the Amerindians, confirming exposure to the parasite. These results, combined with the predominance of IgM antibody responses and the marked detection of secreted products of viable parasites, strongly suggest that recent exposure to T. solium had occurred in the Amerindian population.
Centro de Investigaciones Biomedicas BIOMED Universidad de Carabobo, Maracay, Venezuela.
E
E
Ferrer
M M
MM
Cortez
H
H
Perez
M
M
De la Rosa
B Alarcon
BA
de Noya
I
I
D'Avila
L J S
LJ
Harrison
M
M
Foster-Cuevas
R M E
RM
Parkhouse
A
A
Cabrera
D000293:Adolescent; D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003551:Cysticercosis / Q000453:epidemiology* / Q000209:etiology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007199:Indians, South American; D008297:Male; D020019:Neurocysticercosis / Q000453:epidemiology / Q000209:etiology; D016036:Seroepidemiologic Studies; D013621:Taenia / Q000276:immunology* / Q000302:isolation & purification; D014687:Venezuela / Q000453:epidemiology
D000909:Antibodies, Helminth