Induction of apoptosis in a human erythroleukemic cell line K562 by tylophora alkaloids involves release of cytochrome c and activation of caspase 3.
Tylophora alkaloids are plant products known for their antiasthamatic and antiproliferative activities. The underlying cellular changes resulting from inhibition of proliferation were investigated. Tylophora alkaloids induced apoptosis in K562 cells with characteristic apoptotic features like nuclear condensation, apoptotic body formation, flipping of membrane phosphatidylserine, activation of caspase 3 and release of mitochondrial cytochrome c. These studies suggest that the Tylophora alkaloids, in addition to their antiproliferative effects also induce apoptosis in erythroleukemic cells. These observations imply that Tylophora alkaloids could be useful molecules for their antiproliferative activity and for induction of apoptosis in tumor cells.
Jonaki, BRIT, CCMB, Hyderabad, India.
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Ganguly
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Khar
D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020014:K562 Cells / Q000187:drug effects*; D008928:Mitochondria / Q000378:metabolism*; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D031110:Tylophora
D000470:Alkaloids; D003574:Cytochrome c Group; D010936:Plant Extracts; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases