Immunotoxic catecholamine lesions attenuate 2DG-induced increase of AGRP mRNA.
Injections of the immunotoxin, saporin conjugated to anti-dopamine-beta-hydroxylase (DSAP), into the hypothalamic paraventricular nucleus (PVH) selectively destroy norepinephrine (NE) and epinephrine (E) terminals in the medial hypothalamus and abolish glucoprivic feeding. We utilized PVH DSAP injections to examine the role of NE/E neurons in the previously reported 2-deoxy-D-glucose (2DG)-induced increases in mRNA levels for the orexigenic peptides, AGRP and NPY. Northern blot analysis revealed that DSAP lesions elevated basal but blocked 2DG-induced increases in AGRP mRNA levels. Changes in NPY mRNA were not detectable. AGRP neurons may contribute to circuitry activated by NE/E neurons for elicitation of glucoregulatory responses.
Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6520, USA.
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Fraley
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Ritter
D000199:Actins / Q000378:metabolism; D054369:Agouti-Related Protein; D000818:Animals; D015152:Blotting, Northern; D001835:Body Weight; D002395:Catecholamines / Q000494:pharmacology*; D018076:DNA, Complementary / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism*; D004837:Epinephrine / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism / Q000473:pathology; D000922:Immunotoxins / Q000494:pharmacology*; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D009474:Neurons / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D000199:Actins; D054369:Agouti-Related Protein; D002395:Catecholamines; D018076:DNA, Complementary; D000922:Immunotoxins; D036341:Intercellular Signaling Peptides and Proteins; D009478:Neuropeptide Y; D011506:Proteins; D012333:RNA, Messenger; D012313:RNA; D003847:Deoxyglucose; D009638:Norepinephrine; D004837:Epinephrine