High Immunogenicity of the Pressure-inactivated Virus.
Effects of high hydrostatic pressure on the activity of infectious bursal disease virus (IBDV) was described. The infectivity of IBDV decreased with the increasing of pressure and pressurizing time and may be completely lost, indicated by the tests with chicken embryo fibrablast cell and young chicken as models. How-ever, pressure-inactivated IBDV still remained high immunogenicity and might induce high levels of protecting serum antibody. Fluorescence spectroscopy showed obvious differences between the structures of native and pressurized IBDV, similar with the dissociation-reassociation of oligomeric proteins under high pressure. The changes of pressurized IBDV were also directly observed with electron microscopy. These results suggest that pressure-inactivated IBDV may be used as a vaccine.
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. kcruan@sunm.shcnc.ac.cn
Shao-Min
SM
Tian
Jian-Fei
JF
Qian
Guo-Qing
GQ
Shao
Kang-Cheng
KC
Ruan