High-throughput genetic screening using matrix-assisted laser desorption/ionization mass spectrometry.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become a powerful and widespread analytical tool in all fields of life science. Compared with other techniques, its high accuracy and sensitivity makes it a superior method, especially for the analysis of nucleic acids. Recent problems in the analysis of nucleic acids by MALDI-TOF MS can be solved using an automated MALDI-compatible sample-preparation system. Together with the reliable minisequencing assay, high-throughput genotyping of single nucleotide polymorphisms by MALDI-TOF MS is able to become a routine method in research, clinical genetics and diagnostics.
MWG-Biotech AG, Ebersberg, Germany.
Mikkel
M
Nissum
Dajana
D
Preuss
Anja
A
Harig
Ulrike
U
Lieberwirth
Christian
C
Betz
Susanne
S
Neumann
Elvira
E
Deravanessian
Michael
M
Bock
Lutz
L
Wehmeier
Thomas
T
Bonk
D001331:Automation; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D001523:Mental Disorders / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*