Supplementation of heterologous complement induces anti-Thy-1.1 nephritis in the Mongolian gerbil (Meriones unguiculatus).
Anti-Thy-1.1 nephritis in the rat is a popular experimental model for mesangial proliferative glomerulonephritis (GN). This model is characterized by direct binding of anti-Thy-1.1 antibody with Thy-1.1 antigen expressed on mesangial cells (MCs) of glomeruli in the rat. A single injection of anti-rat thymocyte serum (ARTS) results in GN with proteinuria and extensive mesangiolysis. Development of mesangiolysis and proteinuria are complement-dependent. We previously demonstrated Thy-1.1 antigen, similar to the rat, in thymocytes, brain cells and MCs of the kidney in the Mongolian gerbil (MG). In this study, we attempted to develop a MG nephritis model, but an injection of ARTS did not induce GN. An additional injection of guinea pig serum as a complement after ARTS injection resulted in anti-Thy-1.1 nephritis in MG. Degeneration of MCs and neutrophil infiltration were observed 1 hr after GP serum injection. Mesangiolysis and fibrin exudation occurred 12 hr after the injection and MC proliferation was apparent 7 days after the injection. In the complement-dependent hemolytic test, MG serum could not hemolyze sheep erythrocytes. These results suggested low activity, or depletion of some factors, in complements of MG serum.
Department of Laboratory Animal Science, Nippon Medical School, Tokyo, Japan.
Kazuhiro
K
Shichinohe
Masumi
M
Shimizu-Suganuma
Mohammad
M
Ghazizadeh
Masamichi
M
Ishizaki
D000818:Animals; D003165:Complement System Proteins / Q000494:pharmacology*; D004195:Disease Models, Animal; D005849:Gerbillinae / Q000276:immunology*; D015432:Glomerulonephritis, Membranoproliferative / Q000276:immunology* / Q000473:pathology / Q000662:veterinary*; D006168:Guinea Pigs; D006461:Hemolysis; D007150:Immunohistochemistry / Q000662:veterinary; D007668:Kidney / Q000473:pathology*; D008297:Male; D008854:Microscopy, Electron / Q000662:veterinary; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D018800:Thy-1 Antigens / Q000276:immunology*
D018800:Thy-1 Antigens; D003165:Complement System Proteins