Diagnosis of toxoplasmic retinochoroiditis with atypical clinical features.
OBJECTIVE
To determine the value of aqueous humor analysis for confirming the diagnosis of ocular toxoplasmosis in patients who present with atypical clinical features and to relate the results of local antibody production and polymerase chain reaction (PCR) with the extent of active retinitis and the immune status of the patient.


METHODS
Retrospective case series.


METHODS
Sixty-seven consecutive patients with retinitis or retinochoroiditis that was clinically consistent with atypical ocular toxoplasmosis underwent diagnostic anterior chamber paracentesis and serological studies. The aqueous humor was analyzed both by PCR to detect Toxoplasma gondii B1 gene and by the Goldman-Witmer coefficient to determine levels of local anti-T. gondii antibody production.


RESULTS
In nine of the 67 cases, PCR was positive for T. gondii; seven of these were negative for local antibody production. All nine patients had illnesses associated with immunosuppression or advanced old age and all had active retinitis with a mean area of 11.5 disk areas (DA). Twenty-five of the remaining 58 cases were positive for local antibody production. These 25 had a mean area of active retinitis measuring 2.6 DA, and 24 of these patients were immunocompetent. All 34 cases with laboratory evidence of ocular toxoplasmosis diagnosed by either method responded to anti-T. gondii agents. The remaining 33 were negative for T. gondii infection by these two methods; some had laboratory evidence of other infections.


CONCLUSIONS
Although in the present study, the sensitivity and specificity of the aqueous humor PCR and Goldman-Witmer coefficient could not be ascertained in the laboratory diagnosis of toxoplasmic retinochoroiditis, the PCR method appears to confirm the diagnosis in immunocompromised individuals with large atypical foci of retinitis. Conversely, determination of local antibody production may be appropriate for proper diagnosis in immunocompetent individuals presenting with small foci of retinitis.
Pitié-Salpetriere Hospital Paris VI University, Paris, France.
Christine
C
Fardeau
Stéphane
S
Romand
Narsing A
NA
Rao
Nathalie
N
Cassoux
Olivier
O
Bettembourg
Philippe
P
Thulliez
Phuc
P
Lehoang
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D001082:Aqueous Humor / Q000469:parasitology*; D002825:Chorioretinitis / Q000175:diagnosis* / Q000469:parasitology; D016054:DNA, Protozoan / Q000032:analysis; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014126:Toxoplasmosis, Ocular / Q000175:diagnosis* / Q000469:parasitology
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan