Theoretical mechanisms in targeted and random integration of transgene DNA.
The genetic manipulation of mammalian cells and animals would be greatly expedited if gene targeting could be reliably achieved in the widest possible range of host cell types. This paper considers empirical evidence and theoretical considerations associated with transgene integration, and concludes that utilisation of gene targeting in non-selective systems awaits further progress in modelling homologous recombination.
Smith
K
K
School of Science and Engineering, University of Abertay Dundee, UK. k.smith@abertay.ac.uk
D000818:Animals; D002460:Cell Line; D019457:Chromosome Breakage / Q000235:genetics; D003434:Crossing Over, Genetic / Q000235:genetics; D004247:DNA / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics; D015870:Gene Expression; D018390:Gene Targeting; D015316:Genetic Therapy; D006801:Humans; D008957:Models, Genetic; D011995:Recombination, Genetic; D019076:Transgenes
D004247:DNA