Exchange factors, effectors, GAPs and motor proteins: common thermodynamic and kinetic principles for different functions.
In this article, we review the properties of several classes of proteins that interact with ATPases and GTPases involved in energy and signal transduction. We show that certain common basic thermodynamic principles apply to the manner in which the nucleotide hydrolases interact with their partner molecules, and that the principles involved in signal transduction can be quantitatively modified to give systems with the known properties of energy transducing systems. As an example, actin can be described as an exchange factor for myosin, with its exchange activity being specific for ATP or inorganic phosphate in the myosin.ADP.P(i) complex, in contrast to the unspecific exchange activity of guanosine nucleotide exchange factors operating on GTPases involved in signal transduction and regulatory processes. These common aspects are reflected in shared structural features, suggesting an evolutionary relationship between such systems.
Max-Planck Institute for Molecular Physiology, Department of Physical Biochemistry, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany. goody@mpi-dortmund.mpg.de
Roger S
RS
Goody
Waltraud
W
Hofmann-Goody
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006868:Hydrolysis; D008024:Ligands; D046911:Macromolecular Substances; D008956:Models, Chemical; D009124:Muscle Proteins / Q000378:metabolism*; D011485:Protein Binding; D015398:Signal Transduction; D013816:Thermodynamics
D020690:GTPase-Activating Proteins; D020662:Guanine Nucleotide Exchange Factors; D008024:Ligands; D046911:Macromolecular Substances; D009124:Muscle Proteins; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D020558:GTP Phosphohydrolases