Molecular cloning and characterization of a novel human cAMP response element-binding ( CREB) gene ( CREB4).
Cyclic adenosine monophosphate (cAMP) response element-binding (CREB) proteins are a family of mammalian transcription activators. We identified a novel human CREB gene ( CREB4) that was 1592 bp long and encoded a protein of 395 amino acid residues. The protein shared high homology to mouse CREB3 (identity 62%, similarity 72%). The expression pattern of the human CREB4 gene showed transcripts in prostate, brain, pancreas, skeletal muscle, small intestine, testis, leukocyte, and thymus, whereas in heart, lung, liver, kidney, placenta, spleen, ovary, and colon, specific bands of the transcript could not be detected. The CREB4 gene consisted of ten exons and nine introns and was mapped to chromosome 1q21.3 by means of a bioinformatics analysis.
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, P.R. China, 200433. ymmao@fudan.edu.cn
Gentao
G
Cao
Xiaohua
X
Ni
Min
M
Jiang
Yushu
Y
Ma
Haipeng
H
Cheng
Lingchen
L
Guo
Chaoneng
C
Ji
Shaohua
S
Gu
Yi
Y
Xie
Yumin
Y
Mao
D000595:Amino Acid Sequence; D000818:Animals; D050976:Basic-Leucine Zipper Transcription Factors; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D017362:Cyclic AMP Response Element-Binding Protein; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D009928:Organ Specificity; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics*
D050976:Basic-Leucine Zipper Transcription Factors; C448322:CREB3L4 protein, human; C116472:Creb3 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D009687:Nuclear Proteins; D014157:Transcription Factors