Rapid sample processing and fast gas chromatography for identification of bacteria by fatty acid analysis.
A commercially available system for microbial identification by fatty acid analysis (Microbial Identification System (MIS), MIDI, Newark, DE, USA) requires a four-step sample derivatization procedure in screw-cap test tubes. By using glass tubes in a 96-well format with multichannel pipetting, the time required for sample preparation can be greatly reduced. The standard gas chromatography column, 25 m long by 0.20 mm ID, is replaced with a 10 m long by 0.10 mm ID column, reducing the gas chromatography run time to one third of the standard time. Either or both of these procedures can be easily implemented in any laboratory using the MIDI system, resulting in faster identifications and higher sample throughput.
US Department of Agriculture, ARS, SASL, Building 001 Room 140 BARC-West, Beltsville, MD 20705-2350, USA. buyerj@ba.ars.usda.gov
Jeffrey S
JS
Buyer
D001419:Bacteria / Q000737:chemistry / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*
D005227:Fatty Acids