In vivo cleavage of a target RNA by copper kanamycin A. Direct observation by a fluorescence assay.
A novel fluorescence assay to monitor in vivo cleavage chemistry of RNA target sequences has been established and used to demonstrate the activity of copper aminoglycoside mediated degradation of RNA in bacterial cells.
Chen
Chun-An
CA
Evans Laboratory of Chemistry, Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA.
Cowan
J A
JA
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002240:Carbohydrate Sequence; D003300:Copper / Q000737:chemistry*; D049452:Green Fluorescent Proteins; D007612:Kanamycin / Q000737:chemistry*; D008164:Luminescent Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000900:Anti-Bacterial Agents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D007612:Kanamycin; D012313:RNA; D003300:Copper