Gas chromatographic-mass spectrometric determination of erythrocyte 3-deoxyglucosone in diabetic patients.
To determine if the erythrocyte levels of 3-deoxyglucosone (3-DG) are increased in diabetic patients, and if they correlate with glycemic status, they were measured in diabetic patients without renal disease as well as in healthy subjects. The erythrocyte levels of 3-DG were measured by a selected ion monitoring method of gas chromatography-chemical ionization mass spectrometry using [(13)C(6)]-3-DG as an internal standard. The erythrocyte levels of 3-DG were significantly higher in diabetic patients than in healthy subjects. The erythrocyte concentration of 3-DG was significantly and positively correlated with HbA1c (r=0.84, P<0.001). However, no significant correlation could be found between erythrocyte 3-DG and age, onset age of diabetes, or duration of diabetes in our group of diabetic patients. In diabetes, the production of 3-DG in the erythrocytes is increased via the polyol pathway and/or the Maillard reaction due to hyperglycemia.
Tsukushi
Saori
S
Department of Clinical Preventive Medicine, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8560, Japan.
Kajita
Mitsuharu
M
Nakamura
Sakurako
S
Niwa
Toshimitsu
T
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D012015:Reference Standards
D003847:Deoxyglucose; D006442:Glycated Hemoglobin; C016350:3-deoxyglucosone