T lymphocytes as targets of gene transfer with Moloney-type retroviral vectors.
Peripheral T lymphocytes are a target of choice for many gene therapeutic strategies. Retrovirus-mediated transduction allows genomic integration and long-term expression of transgenes in target cells. Over many years, low transduction efficiency into primary T lymphocytes has limited clinical application of existing protocols. Recently, gene transfer rates > 50% have been achieved facilitating clinical studies. More attention is thus being focused on the ability of gene-modified cells to carry out innate as well as conferred functions in vivo and the influence of culture conditions, retroviral vector and host response thereon.
Ayuk
F A
FA
University Hospital Eppendorf, Martinistr. 52, 20251 Hamburg, Germany.
Zander
A R
AR
Fehse
B
B
D000818:Animals; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D008979:Moloney murine leukemia virus / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D013601:T-Lymphocytes / Q000502:physiology*; D014161:Transduction, Genetic