Isophosphoramide mustard analogues as prodrugs for anticancer gene-directed enzyme-prodrug therapy (GDEPT).
Two types of prodrugs, benzyl analogues of isophosphoramide mustard (iPAM), activated by cytochrome P450, and acylthioethyl analogues, activated by esterases, were designed. In contrast to iPAM that hydrolyse rapidly, the examined compounds are stable in phosphate-buffered saline and Tris buffer. Benzyl analogues of iPAM are poor substrates for cytochrome P450, are not cytotoxic and posses no antitumour activity. Acylthioethyl analogues of iPAM are good substrates for pig liver esterase, are cytotoxic and exert antitumour activity against L1210 leukaemia in mice. The observed correlation for iPAM analogues between their susceptibility to hydrolysis and cytotoxicity and antitumour activity suggests possible application of these compounds as the prodrugs in gene-directed enzyme-prodrug therapy.
Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lódź. kmisiura@bio.cbmm.lodz.pl
Konrad
K
Misiura
Daria
D
Szymanowicz
Halina
H
Kuśnierczyk
Joanna
J
Wietrzyk
Adam
A
Opolski
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002277:Carcinoma / Q000188:drug therapy; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004355:Drug Stability; D004950:Esterases; D004952:Esters; D006801:Humans; D007069:Ifosfamide; D007938:Leukemia / Q000188:drug therapy*; D051379:Mice; D009062:Mouth Neoplasms / Q000188:drug therapy; D009711:Nucleotides; D010752:Phosphoramide Mustards / Q000737:chemistry*; D011355:Prodrugs / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D004952:Esters; D009711:Nucleotides; D010752:Phosphoramide Mustards; D011355:Prodrugs; C027061:isophosphamide mustard; D003577:Cytochrome P-450 Enzyme System; D004950:Esterases; D007069:Ifosfamide