Direct monitoring of DNA cleavages catalyzed by an ATP-dependent deoxyribonuclease on a 27 MHz quartz-crystal microbalance.
Kinetic studies of enzymatic DNA cleavage reactions (the enzyme binding, hydrolysis along DNA strands, and then release of the enzyme from the completely hydrolyzed ssDNA) were carried out on a 27 MHz quartz-crystal microbalance.
Matsuno
Hisao
H
Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuda, Midori-ku, Yokohama 226-8501, CREST, JST (Japan Science and Technology Corporation), Japan.
Furusawa
Hiroyuki
H
Okahata
Yoshio
Y
D000255:Adenosine Triphosphate; D004247:DNA / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008832:Microchemistry / Q000295:instrumentation*
D000255:Adenosine Triphosphate; D004247:DNA; D003851:Deoxyribonucleases