[The study of pro-nucleating activity on 33.5 x 10(3) vesicular protein in bile].
OBJECTIVE
To evaluate the pro-nucleating activity in 33.5 x 10(3) vesicular protein.


METHODS
The model biles were established according Kibe and Zhu. The pro-nucleating activity of 33.5 x 10(3) vesicular protein were examined by polarized light microscopy. The protein and its enzymatic deglycosylation and proteolysis fractions nucleation promoting activity were detected by cholesterol crystal growth assay.


RESULTS
33.5 x 10(3) vesicular protein displayed apparent potency of pro-nucleation with activity of 0.310, and derived crystal growth curve indices It, Ig, Ic were presented as 0.57, 1.52, 1.63 respectively, but after treated by N-glycanase enzyme and pronase, no promoting activity were found.


CONCLUSIONS
The 33.5 x 10(3) vesicular protein may be involved in the nucleation process of gallstone formation, which is regulated by its peptide and sugar chain.
Department of Surgery, Huashan Hospital of Fudan University, Shanghai 200040, China.
Baojin
B
Ma
Jianbin
J
Xiang
Duan
D
Cai
Yanling
Y
Zhang
Xiliang
X
Zha
Liying
L
Wang
D001646:Bile / Q000378:metabolism*; D002769:Cholelithiasis / Q000503:physiopathology*; D002784:Cholesterol / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D008853:Microscopy; D008954:Models, Biological; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism
D046911:Macromolecular Substances; D011506:Proteins; D002784:Cholesterol