Effects of replacing the movement protein gene of Tobacco mosaic virus by that of Tomato mosaic virus.
The broad bean strain of Tobacco mosaic virus (TMV-B) infects Nicotiana tabacum White Burley systemically whereas the tomato strain of T. mosaic virus (ToMV-S1) induces necrotic local lesions and is restricted to inoculated leaves. To examine the possible role of the viral movement protein (MP) in these symptom differences, a chimaeric virus (T/OMP) was produced in which the TMV-B MP gene was replaced by the ToMV-S1 MP gene. T/OMP induced the same symptoms as TMV-B in N. tabacum White Burley. However, in N. tabacum Samsun NN and other plants containing the N resistance gene, T/OMP caused necrotic lesions that were smaller than those produced by TMV-B but similar in size to those of ToMV-S1. We conclude that ToMV MP gene can substitute functionally for the TMV-B MP gene, and that the MP gene influences the size of necrotic local lesions on N-containing hosts.
Institute of Biotechnology, Zhejiang University, Hangzhou, People's Republic of China.
Junjie
J
Wu
Xueping
X
Zhou
D001483:Base Sequence; D004279:DNA, Viral; D005814:Genes, Viral / Q000502:physiology*; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D010935:Plant Diseases; D018515:Plant Leaves / Q000821:virology; D053830:Plant Viral Movement Proteins; D014026:Nicotiana; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000502:physiology
D004279:DNA, Viral; D053830:Plant Viral Movement Proteins; D014764:Viral Proteins