The Regulation of Xmyf-5 Gene, a Muscle Determinant, Expression in Xenopus Laevis.
Xmyf-5 gene is a member of the myogenic regulatory genes important for Xenopus muscle cell commitment and differentiation. To analyze the mechanism involved in regulating Xmyf-5 gene activity in the process of myogenesis, the 4.9 kb 5' upstream sequence of Xmyf-5 was identified. LacZ gene could be activated in the presomitic mesoderm in stage 14 embryos and myotomes in stage 25 embryos when it was driven by the 4.9 kb sequence and introduced into embryos at 2 cell stage. The Luciferase reporter gene activities driven by deletion constructs suggested that there are at least one enhancer between 4.9 kb and 3.5 kb of Xmyf-5 5' upstream DNA and one negatively regulative factor between 2.0 kb and 0.9 kb of the Xmyf-5 5' upstream DNA. In addition, sequence analysis indicated that a 100 bp fragment 5' upstream of Xmyf-5 contained conserved regulative elements such as OLS which were found in other vertebrates.
Shanghai Institute of Cell Biology, the Chinese Academy of Sciences, Shanghai 200031, China. xyding@sunm.shcnc.ac.cn
Wen-Yan
WY
Mei
Jing
J
Yang
Qing-Hua
QH
Tao
Xiao-Yan
XY
Ding