Identification and molecular cloning of Xenopus laevis SP22, a protein associated with fertilization in mammals.
SP22 is a novel sperm protein that has been shown to be highly correlated with fertility in rats. SP22 homologues have been studied in mouse and man but a definitive role for the protein has not yet been established. Using a polyclonal IgG to recombinant rat SP22, we detected the presence of this protein in Xenopus laevis tissues. Moreover, a Xenopus EST was found that shares a high degree of similarity with rat SP22 and the derived sequence codes for an 189 aa protein that is very similar to rat SP22. Finally, using Western blotting and RT-PCR analyses, we investigated the expression of Xenopus SP22 both in adult tissues and during embyronic development. SP22 protein expression predominated in the adult testis, and both mRNA and protein levels diminished subsequent to the initial day following fertilization.
Monetti
Claudio
C
Dipartimento di Biologia Strutturale e Funzionale, Università degli Studi dell'Insubria, Via J.H. Dunant 3, I-21100, Varese, Italy
Vigetti
Davide
D
Gornati
Rosalba
R
Prati
Mariangela
M
Klinefelter
Gary R
GR
Bernardini
Giovanni
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D005306:Fertilization; D006801:Humans; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D000071617:Protein Deglycase DJ-1; D051381:Rats; D016415:Sequence Alignment; D013094:Spermatozoa / Q000378:metabolism; D014018:Tissue Distribution; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics / Q000378:metabolism
D008869:Microtubule-Associated Proteins; C491281:PRDX3 protein, Xenopus; C491283:SP22 protein, human; C491282:SP22 protein, mouse; D029867:Xenopus Proteins; C404267:PARK7 protein, rat; D000071617:Protein Deglycase DJ-1