Discriminatory power of RAPD, PCR-RFLP and southern blot analyses of ureCD or ureA gene probes on Helicobacter pylori isolates.
The genetic diversity of 33 Nigerian Helicobacter pylori isolates were studied using RAPD, PCR-RFLP and Southern blot analysis of ureA or ureCD gene probes. RAPD was able to distinguish the following number of isolates using the primers 3880: 5'-AAGAGCCCGT-3' (28), 3881 :5'-AACGCGCAAC-3' (33) and OPH8 :5'-GAAACACCCC-3' (25). Southern blot analysis using the ureCD probe was also able to distinguish the 12 isolates tested into ten different patterns. The PCR-RFLP technique distinguished all 33 isolates into six types. In conclusion, considering typeability, discriminatory power, and convenience, RAPD with the 3881 primer was considered the most useful technique.
Smith
Stella
S
Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Yaba, Lagos. stellaismith@yahoo.com
Cantet
Franck
F
Angelini
Fabrice
F
Marais
Armelle
A
Mégraud
Francis
F
Bayerdöffer
Ekkhehard
E
Miehlke
Stephan
S
D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers; D005756:Gastritis / Q000382:microbiology; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D009549:Nigeria; D010733:Phosphoglucomutase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D012680:Sensitivity and Specificity; D014456:Ulcer / Q000382:microbiology; D014510:Urease / Q000235:genetics*
D001426:Bacterial Proteins; D017931:DNA Primers; C086767:UreD protein, Bacteria; D014510:Urease; C097631:phosphoglucosamine mutase; D010733:Phosphoglucomutase