BCR mutants deficient in ligand-independent and more sensitive for ligand-dependent signaling.
Signal transduction by the B cell antigen receptor (BCR) regulates development, survival and clonal expansion of B cells. The BCR complex comprises the membrane-bound immunoglobulin molecule (mIg) and the Ig-alpha/Ig-beta heterodimer, and was shown to form oligomeric structures. In pervanadate (PV)-treated B cells, multiple proteins are tyrosine phosphorylated upon expression of the BCR, indicating that the BCR can signal in an antigen-independent fashion. We analyzed the signal transduction from BCR mutants which either have an altered heavy chain transmembrane region or lack the Ig-alpha cytoplasmic tail. In comparison to cells expressing the wild-type receptors, those with a mutant BCR respond to PV treatment with reduced and retarded tyrosine phosphorylation of substrate proteins. Conversely, the cells with mutant BCR are more sensitive to stimulation with low doses of antigen. These data suggest that a correctly assembled BCR complex is important for antigen-independent signaling and setting the threshold for antigen-dependent BCR activation.
Department of Molecular Immunology, Biology III, University of Freiburg, and Max-Planck-Institute for Immunobiology, Freiburg, Germany.
Catrin
C
Pracht
Kerstin
K
Gimborn
Michael
M
Reth
Michael
M
Huber
D000941:Antigens / Q000276:immunology; D006801:Humans; D007072:Immunoglobulin D / Q000502:physiology; D009154:Mutation; D010766:Phosphorylation; D011947:Receptors, Antigen, B-Cell / Q000502:physiology*; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology
D000941:Antigens; D007072:Immunoglobulin D; D011947:Receptors, Antigen, B-Cell; C076715:pervanadate; D014638:Vanadates; D014443:Tyrosine