Synthesis of Galacto-oligosaccharide in Two-phase System.
35 of the total products of galacto-oligosaccharide (GOS) could be obtained from the two-phase system with cyclohexane and ethyl acetate as bulk organic phases and 15% phosphate buffer as aqueous phase. The effects of temperature pH of buffer lactose concentration galactose and glucose and the immobilization of enzyme on the synthesis of GOS were studied. It was found that the reaction temperature and initial lactose concentration didn'thave obvious effects while the addition of glucose and galactose somewhat affected the GOS yield and the GOS yields could reach 64.78% with lactase immobilized on resin D345.
State Key Laboratory of Bioreactor Engineering Research Institute of Biochemistry East China University of Science and Technology Shanghai 200237 China. dzhwei@ecust.edu.cn
Li-Qiong
LQ
Gui
Dong-Zhi
DZ
Wei
Yu-Min
YM
Cui
Jun-Tang
JT
Yu