Intermolecular interactions, nucleation, and thermodynamics of crystallization of hemoglobin C.
The mutated hemoglobin HbC (beta 6 Glu-->Lys), in the oxygenated (R) liganded state, forms crystals inside red blood cells of patients with CC and SC diseases. Static and dynamic light scattering characterization of the interactions between the R-state (CO) HbC, HbA, and HbS molecules in low-ionic-strength solutions showed that electrostatics is unimportant and that the interactions are dominated by the specific binding of solutions' ions to the proteins. Microscopic observations and determinations of the nucleation statistics showed that the crystals of HbC nucleate and grow by the attachment of native molecules from the solution and that concurrent amorphous phases, spherulites, and microfibers are not building blocks for the crystal. Using a novel miniaturized light-scintillation technique, we quantified a strong retrograde solubility dependence on temperature. Thermodynamic analyses of HbC crystallization yielded a high positive enthalpy of 155 kJ mol(-1), i.e., the specific interactions favor HbC molecules in the solute state. Then, HbC crystallization is only possible because of the huge entropy gain of 610 J mol(-1) K(-1), likely stemming from the release of up to 10 water molecules per protein intermolecular contact-hydrophobic interaction. Thus, the higher crystallization propensity of R-state HbC is attributable to increased hydrophobicity resulting from the conformational changes that accompany the HbC beta 6 mutation.
Department of Chemical Engineering, University of Houston, Houston, Texas 77204, USA. vekilov@uh.edu
Peter G
PG
Vekilov
Angela R
AR
Feeling-Taylor
Dimiter N
DN
Petsev
Oleg
O
Galkin
Ronald L
RL
Nagel
Rhoda Elison
RE
Hirsch
D000465:Algorithms; D055592:Biophysical Phenomena; D001703:Biophysics; D006444:Hemoglobin C / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008027:Light; D009154:Mutation; D010108:Oxyhemoglobins / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D012542:Scattering, Radiation; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D013816:Thermodynamics
D010108:Oxyhemoglobins; D012965:Sodium Chloride; D006444:Hemoglobin C