Application of Free-flow Electrophoresis to the Separation of Murine Lymphocytes.
Free-flow electrophoresis offers not only the all-in-solution separation process but also very gentle separation conditions, and it can be used for continuous separation and preparation, so it has been widely applied in the fields of biochemistry and cell biology. Using this technique to separate insoluble particles such as cells, both high separation efficiency and high activity and viability could be obtained. Murine lymphocytes were separated in low ionic strength triethanolamine buffer by free-flow electrophoresis, the cell fractions were detected and characterized by means of UV spectrometry, immune fluorescence labeling and flow cytometry. Results indicated that T and B lymphocytes were well separated with high viability.
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. xiaqc@sunm.shcnc.ac.cn
Tao
T
Liu
Xin
X
Shen
Gao-De
GD
Wu
Qi-Chang
QC
Xia
Sheng-Nian
SN
Wang
Zhi-Jiang
ZJ
Wu
Wei
W
Hu