Process development for heme-enriched peptide by enzymatic hydrolysis of hemoglobin.
An efficient production method of heme-iron-enriched peptide was developed based on enzymatic hydrolysis. Hemoglobin hydrolysis, carried out stepwise with commercially available exopeptidase and endopeptidase, resulted in an increased degree of hydrolysis (DH). Exopeptidase-catalyzed protein hydrolysis formed low molecular weight peptides and amino acids. Different process parameters including dialysis and ultra- and diafiltration were evaluated. Heme/peptide ratio increased as molecular weight cut-off (MWCO) of the dialysis membrane increased. When the hydrolysate was dialyzed against sodium phosphate buffer, a higher heme/ peptide ratio was obtained. The heme/peptide ratio of the hydrolysate reached up to 25.4% when the dialysis was carried out with a membrane of 12-14 kDa MWCO. Also, the ratio was improved by the use of ultrafiltration and diafiltration on the pilot-scale.
In
Man-Jin
MJ
Department of Human Nutrition and Food Science, Chungwoon University, Hongsung, Republic of Korea.
Chae
Hee Jeong
HJ
Oh
Nam-Soon
NS
D003956:Dialysis / Q000379:methods*; D010450:Endopeptidases / Q000737:chemistry*; D006418:Heme / Q000737:chemistry*; D006420:Hemeproteins / Q000138:chemical synthesis*; D006454:Hemoglobins / Q000737:chemistry*; D006868:Hydrolysis; D010710:Phosphates / Q000737:chemistry; D010865:Pilot Projects; D016062:Porosity; D011786:Quality Control; D012680:Sensitivity and Specificity; D012697:Serine Endopeptidases / Q000737:chemistry*; D014325:Tromethamine / Q000737:chemistry; D014462:Ultrafiltration / Q000379:methods*
D006420:Hemeproteins; D006454:Hemoglobins; D010710:Phosphates; D014325:Tromethamine; D006418:Heme; D010450:Endopeptidases; C412470:flavourzyme; D012697:Serine Endopeptidases; C021553:mesentericopeptidase; C018279:sodium phosphate