Plating densities, alpha-difluoromethylornithine effects and time dependence on the proliferation of IEC-6 cells.
OBJECTIVE
To characterize the role of plating densities and alpha-difluoromethylornithine (DFMO) on the proliferation of IEC-6 cells in vitro.


METHODS
IEC-6 cells were seeded in 96-well microplates at various densities in the presence or absence of DFMO. Cells were counted and their proliferative capability was monitored Days 1 to 7 with MTT assay at an optical density of 570 nm.


RESULTS
There was a positive relationship between cell number and OD value (r = 0.954, P < 0.01). Higher plating densities (> 0.5 x 10(4) cells/well) inhibited the growth of cells on Day 2. When the density reaches 4 x 10(4) cells/well, the OD value increased gradually and reached a peak on Day 5. After that, the OD value began to fall. The growth of IEC-6 cells was limited at a low density (0.2 x 10(4) cells/well) on Day 4. DFMO caused a complete inhibition of proliferation of IEC-6 cells on Days 1 to 3.


CONCLUSIONS
Proliferation of IEC-6 cells is related to plating density and incubation time. It is inhibited by DFMO, but is reversible when the incubation time is prolonged.
Zhang
Zili
Z
Piwei Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Chen
Weiwen
W
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D000518:Eflornithine / Q000494:pharmacology*; D013997:Time Factors
D000970:Antineoplastic Agents; D000518:Eflornithine