Reproduction disturbances of Brachionus calyciflorus (rotifer) for the screening of environmental endocrine disrupters.
Rotifers and especially the Brachionus calyciflorus species have already shown advantages (e.g. good sensitivity, simplicity, ecological relevance, short generation time) for the chronic toxicity assessment of water pollutants. In this study, the B. calyciflorus three-day test was evaluated on ethinylestradiol, nonylphenol and testosterone for the determination of endocrine disrupting (reproductive) effects. Reproduction (intrinsic rate of population increase) and sex ratio (males/females and mictic females/amictic females) were explored and discussed as suitable endpoints. B. calyciflorus has shown a fair sensitivity to detect reproductive disrupting effects after exposure to ethinylestradiol, nonylphenol and testosterone. Population growth (intrinsic rate of population increase, r) and the ratio ovigerous females/ non-ovigerous females are both suitable endpoints, whereas the occurrence of males was not frequent enough to be used as endpoint.
GSF--Forschungszentrum für Umwelt und Gesundheit, Institut für Okologische Chemie, Neuherberg, Germany. pascal.radix@aventis.com
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P
Radix
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Severin
K W
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Schramm
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A
Kettrup
D000818:Animals; D004703:Endocrine System / Q000187:drug effects*; D023381:Endpoint Determination; D005260:Female; D012739:Gonadal Steroid Hormones / Q000009:adverse effects; D008297:Male; D011157:Population Dynamics; D012098:Reproduction / Q000187:drug effects*; D012403:Rotifera; D012680:Sensitivity and Specificity; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
D012739:Gonadal Steroid Hormones; D014874:Water Pollutants, Chemical