Effects of the nitric oxide donor molsidomine on different memory components as assessed in the object-recognition task in the rat.
BACKGROUND
Nitric oxide (NO) is sought to be a novel intracellular messenger in the central nervous system. Recently, NO involvement in learning and memory processes has been proposed. Compounds that inhibit NO synthase, the key synthesizing enzyme, may block cognition, while NO donors may facilitate it.


OBJECTIVE
The present study was designed to further investigate in the rat the effects on distinct memory processes exerted by the NO donor molsidomine. For this aim, the object-recognition task was chosen. This test is based on the differential exploration of a new and familiar object.METHODS. Object recognition was evaluated in a two-trial nonrewarded paradigm. In a first study, the influence of the retention time (the delay between the two trials) on the performance of 3-month-old male rats was assessed. Subsequently, the effects of molsidomine (2 mg/kg and 4 mg/kg), injected i.p., on acquisition, storage, and retrieval of information were evaluated. For the latter experiments, the delay condition at which recognition memory was extinguished in the normal rat was used (24 h).


RESULTS
Under our experimental conditions, object recognition was extinguished in the rat when an intertrial interval (ITI) of 24 h was utilized. Using this ITI, molsidomine at 4 mg/kg but not at 2 mg/kg improved the animal's performance in the object-recognition task, suggesting that molsidomine affected acquisition, storage, and retrieval of information.


CONCLUSIONS
These results indicate that the NO donor molsidomine may modulate different aspects of memory.
Department of Medical Pharmacology, University of Milan, via Vanvitelli 32, 20129 Milan, Italy. nikpit@yahoo.it
Nikolaos
N
Pitsikas
Antonello E
AE
Rigamonti
Silvano G
SG
Cella
Eugenio E
EE
Muller
D000818:Animals; D004192:Discrimination, Psychological / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology; D008297:Male; D008568:Memory / Q000187:drug effects*; D008981:Molsidomine / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D011597:Psychomotor Performance / Q000187:drug effects*; D051381:Rats; D013997:Time Factors
D020030:Nitric Oxide Donors; D008981:Molsidomine