Peptidomic approaches in proteomic research.
Peptides play a central role in many physiological processes. In order to comprehensively analyze all peptides and small proteins of a whole organism or a subsystem (peptidome), technologies other than 2-D gel electrophoresis are required. Although systematic efforts directed at peptides and peptidomes, comparable to the numerous proteomics projects, are largely lacking to date, a number that employ liquid chromatography or affinity purification and mass spectrometric identification have now been developed and applied successfully to the analysis of a variety of different peptide sources. Furthermore, distinct peptide classes, such as antimicrobial peptides or peptides related to metabolic diseases such as diabetes/obesity, are once again receiving attention. Here we discuss peptides in terms of their applicability to serve as diagnostic markers (or more generally as biomarkers), as well as therapeutic targets or lead compounds. There are also a number of technological challenges that need to be overcome in the study of potent animal venoms and plant toxins, both of which are generally peptides and which are discussed as potential lead compounds for therapeutic intervention in diseases such as cancer.
Jürgens
Michael
M
BioVisioN AG, Hannover, Germany. m.juergens@biovision.de
Schrader
Michael
M
D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers; D001826:Body Fluids / Q000737:chemistry; D002853:Chromatography, Liquid; D015195:Drug Design; D006801:Humans; D013058:Mass Spectrometry; D008659:Metabolic Diseases / Q000175:diagnosis; D036361:Peptide Hormones / Q000737:chemistry / Q000627:therapeutic use; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification / Q000502:physiology; D010641:Phenotype; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D014118:Toxins, Biological / Q000737:chemistry / Q000627:therapeutic use
D015415:Biomarkers; D036361:Peptide Hormones; D010455:Peptides; D020543:Proteome; D014118:Toxins, Biological