Blood coagulation factor X (FX) in human seminal plasma.
The active form of human blood coagulation factor X (FXa, EC 3.4.21.6) showing N-alpha-Benzoyl-L-isoleucyl-L-glutamyl-L-glycyl-L-arginine- p-nitroanilide (S-2222) hydrolyzing activity was first detected in human semen (seminal plasma) by affinity chromatography using anti-human coagulation factor X, and this enzyme activity was inhibited by anti-human FX. This enzyme has been associated with the human coagulation factor X (FX) in human semen (seminal plasma) by Western blot analysis, and the molecular mass of mature FX was also estimated to be 59 KDa by SDS-PAGE and Western blot analysis.
Meiji Pharmaceutical University, Kiyose-shi, Tokyo, Japan.
Y
Y
Matsuda
K
K
Shimokawa
M
M
Katayama
H
H
Shimizu
T
T
Umeda
S
S
Oshio
R
R
Chiba
D015153:Blotting, Western; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005170:Factor X / Q000008:administration & dosage* / Q000378:metabolism; D015951:Factor Xa / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008297:Male; D009842:Oligopeptides / Q000378:metabolism; D012661:Semen / Q000737:chemistry*
D009842:Oligopeptides; C015608:benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide; D005170:Factor X; D015951:Factor Xa