Ubiquitin ligase-associated protein SGT1 is required for host and nonhost disease resistance in plants.
Homologues of the yeast ubiquitin ligase-associated protein SGT1 are required for disease resistance in plants mediated by nucleotide-binding site/leucine-rich repeat (NBS-LRR) proteins. Here, by silencing SGT1 in Nicotiana benthamiana, we extend these findings and demonstrate that SGT1 has an unexpectedly general role in disease resistance. It is required for resistance responses mediated by NBS-LRR and other R proteins in which pathogen-derived elicitors are recognized either inside or outside the host plant cell. A requirement also exists for SGT1 in nonhost resistance in which all known members of a host species are resistant against every characterized isolate of a pathogen. Our findings show that silencing SGT1 affects diverse types of disease resistance in plants and support the idea that R protein-mediated and nonhost resistance may involve similar mechanisms.
Peart
Jack R
JR
The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, United Kingdom.
Lu
Rui
R
Sadanandom
Ari
A
Malcuit
Isabelle
I
Moffett
Peter
P
Brice
David C
DC
Schauser
Leif
L
Jaggard
Daniel A W
DA
Xiao
Shunyuan
S
Coleman
Mark J
MJ
Dow
Max
M
Jones
Jonathan D G
JD
Shirasu
Ken
K
Baulcombe
David C
DC
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D020868:Gene Silencing; D005822:Genetic Vectors; D008025:Ligases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010935:Plant Diseases; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010942:Plant Viruses; D012328:RNA Viruses; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics; D044767:Ubiquitin-Protein Ligases
D002352:Carrier Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins; C085200:R protein, Zea mays; D044767:Ubiquitin-Protein Ligases; D008025:Ligases