Recovery of virulent and RNase-negative attenuated type 2 bovine viral diarrhea viruses from infectious cDNA clones.
Cloned cDNA derived from the genome of the virulent type 2 bovine viral diarrhea virus (BVDV) strain NY'93/C was sequenced and served for establishment of the infectious cDNA clone pKANE40A. Virus recovered from pKANE40A exhibited growth characteristics similar to those of wild-type BVDV NY'93/C and proved to be clinically indistinguishable from the wild-type virus in animal experiments. A virus mutant in which the RNase residing in the viral glycoprotein E(rns) was inactivated, revealed an attenuated phenotype. The plasmid pKANE40A represents the first infectious cDNA clone established for a type 2 BVDV and offers a variety of new approaches to analyze the mechanisms of BVDV-induced disease in cattle.
Meyer
Christiane
C
Institute of Immunology, Federal Research Centre for Virus Diseases of Animals, D-72001 Tübingen, Germany.
Von Freyburg
Martina
M
Elbers
Knut
K
Meyers
Gregor
G
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000209:etiology / Q000276:immunology / Q000821:virology; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D001908:Diarrhea Viruses, Bovine Viral / Q000201:enzymology / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D005260:Female; D005334:Fever / Q000209:etiology; D007958:Leukocyte Count; D009154:Mutation; D009500:Neutralization Tests; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism; D014774:Virulence
D000914:Antibodies, Viral; D018076:DNA, Complementary; D004279:DNA, Viral; D012260:Ribonucleases