[Increase expression of CYP2E1 mRNA after methamphetamine administration in rats].
Methamphetamine (METH) is one of the most commonly abused psychostimulant. We have investigated the effect of METH administration on the CYP2E1 mRNA level using quantitative competitive reverse transcription-polymerase chain reaction assay. Rats were given METH (4 mg/kg or 8 mg/kg/day) for 2 weeks. The expression of CYP2E1 mRNA increased (5.7 fold) in 4 mg/kg/day administration rat liver in compared with control rat liver. The results suggest that exposure to METH may increase CYP2E1 level but superfluous exposure (8 mg/kg/day) of METH may reduce the expression of CYP2E1 gene.
Division of Neurophysiology, Graduate School of Azabu University, 1-17-7 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
Takahiro
T
Takimoto
Hiroshi
H
Ujike
Kazuhiko
K
Nakamura
Kazuhiko
K
Iwahashi
D000818:Animals; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D019392:Cytochrome P-450 CYP2E1 / Q000096:biosynthesis* / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar
D000697:Central Nervous System Stimulants; D012333:RNA, Messenger; D008694:Methamphetamine; D019392:Cytochrome P-450 CYP2E1