Structure of beta-cinnamomin, a protein toxic to some plant species.
Phytophthora and Pythium species are among the most aggressive plant pathogens, as they invade many economically important crops and forest trees. They secrete large amounts of 10 kDa proteins called elicitins that can act as elicitors of plant defence mechanisms. These proteins may also induce a hypersensitive response (HR) including plant cell necrosis, with different levels of toxicity depending on their pI. Recent studies showed that elicitins function as sterol carrier proteins. The crystallographic structure of the highly necrotic recombinant beta-cinnamomin (beta-CIN) from Phytophthora cinnamomi has been determined at 1.8 A resolution using the molecular-replacement method. beta-CIN has the same overall structure as beta-cryptogein (beta-CRY), an elicitin secreted by Phytophthora cryptogea, although it shows a different surface electrostatic potential distribution. The protein was expressed in Pichia pastoris and crystallized in the triclinic space group with two monomers in the asymmetric unit. The interface formed by these two monomers resembles that from beta-CRY dimer, although with fewer interactions.
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, ITQB-UNL, Av. República, Apt 127, 2781-901 Oeiras, Portugal.
Maria L
ML
Rodrigues
Margarida
M
Archer
Paulo
P
Martel
Alain
A
Jacquet
Alfredo
A
Cravador
Maria A
MA
Carrondo
D020418:Algal Proteins / Q000737:chemistry* / Q000235:genetics / Q000633:toxicity; D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D005656:Fungal Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010838:Phytophthora / Q000737:chemistry / Q000235:genetics / Q000472:pathogenicity; D010944:Plants / Q000187:drug effects; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000633:toxicity; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D054790:Ribosome Inactivating Proteins, Type 2; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity
D020418:Algal Proteins; D005656:Fungal Proteins; D011506:Proteins; D011994:Recombinant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; C061032:cryptogein protein, Phytophthora cryptogea; C085101:elicitin, Phytophthora; C061930:cinnamomin, Phytophthora cinnamomi