Enantioselective analysis of glufosinate using precolumn derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate and reversed-phase liquid chromatography.
We have developed a new analytical method to quantify the DL-homoalanine-4-yl(methyl)phosphinate (DL-GLUF) enantiomers in biological specimens using a reversed-phase high-performance liquid chromatography system with a fluorescence detection system. The derivatization of DL-GLUF enantiomers with (+)-1-(9-fluorenyl)ethyl chloroformate was carried out under mild conditions (40 degrees C for 30 min) without inducing racemization. The lower limit of quantitation was 0.01 microg/ml for both D-GLUF and L-GLUF, and the detection limit was 5 ng/ml. When DL-GLUF enantiomers were added to serum to produce concentrations between 0.1 and 100 microg/ml, the mean recovery rate was at least 93.8%. The recovery rate from urine was also satisfactory.
Department of Analytical Chemistry, Niigata College of Pharmacy, Niigata, Japan. horiy@xa3.so-net.ne.jp
Yasushi
Y
Hori
Manami
M
Fujisawa
Kenji
K
Shimada
Mitsuru
M
Sato
Masao
M
Honda
Yasuo
Y
Hirose
D000613:Aminobutyrates / Q000032:analysis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005449:Fluorenes / Q000737:chemistry*; D006540:Herbicides / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D000613:Aminobutyrates; D005449:Fluorenes; D006540:Herbicides; D007202:Indicators and Reagents; C052812:1-(9-fluorenyl)ethyl chloroformate; C003121:phosphinothricin