Heterologous expression and purification of SpaB involved in subtilin biosynthesis.
Lantibiotic peptides contain thioether bridges termed lanthionines that are putatively generated by dehydration of Ser and Thr residues followed by Michael addition of cysteine residues within the peptide. The LanB and LanC proteins have been proposed to catalyze the dehydration and formation of the thioether rings, respectively. We report here the first heterologous overexpression in Escherichia coli of SpaB, the putative dehydratase for subtilin. Sequence analysis of spaB revealed several nucleotide differences with current gene database entries. The solubility of SpaB was increased dramatically when co-expressed with GroEL/ES, and soluble His(6)-tagged SpaB was purified. The protein is at least a dimer, and interaction between SpaB and SpaC was observed. SpaS the putative substrate for SpaB was overexpressed in E. coli as an intein fusion protein, and after cleavage, the peptide was obtained in good yield.
Xie
Lili
L
Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Ave, Urbana, IL 61801, USA.
Chatterjee
Champak
C
Balsara
Rashna
R
Okeley
Nicole M
NM
van der Donk
Wilfred A
WA
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001426:Bacterial Proteins; D001430:Bacteriocins; D018834:Chaperonin 60 / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000378:metabolism; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000302:isolation & purification*; D008956:Models, Chemical; D010455:Peptides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D020798:Two-Hybrid System Techniques
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D018834:Chaperonin 60; D008565:Membrane Proteins; D010455:Peptides; C104626:SpaB protein, Bacillus subtilis; C002836:subtilin