Alpha-N-acetylgalactosaminidase from marine bacterium Arenibacter latericius KMM 426T removing blood type specificity of A-erythrocytes.
An alpha-N-acetylgalactosaminidase IV able to remove blood type specificity of human A(II)-erythrocytes and not effecting B(III)-erythrocytes was isolated from the marine bacterium Arenibacter latericius KMM 426T. The alpha-N-acetylgalactosaminidase IV preparation exhibits high activity during inhibition of hemagglutination with blood group substance A containing determinants analogous to A-erythrocytes. The enzyme has a pH optimum from 7.0 to 8.0 and completely retains its activity during 30-min heating at 50 degrees C and for a week at 20 degrees C. The enzyme can be stored under the sterile conditions for any length of time at 4 degrees C, but it does not withstand freezing. The alpha-N-acetylgalactosaminidase is resistant to NaCl; for p-nitrophenyl-alpha-N-acetyl-D-galactosaminide, the Km is 0.38 mM. The molecular mass of the enzyme determined by gel filtration is 84 kD.
Bakunina
I Yu
IY
Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia.
Kuhlmann
R A
RA
Likhosherstov
L M
LM
Martynova
M D
MD
Nedashkovskaya
O I
OI
Mikhailov
V V
VV
Elyakova
L A
LA
D000017:ABO Blood-Group System / Q000276:immunology*; D001426:Bacterial Proteins / Q000378:metabolism*; D002240:Carbohydrate Sequence; D004912:Erythrocytes / Q000276:immunology*; D016946:Gram-Negative Aerobic Rods and Cocci / Q000201:enzymology*; D006596:Hexosaminidases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D048809:alpha-N-Acetylgalactosaminidase
D000017:ABO Blood-Group System; D001426:Bacterial Proteins; D006596:Hexosaminidases; C482722:NAGA protein, human; D048809:alpha-N-Acetylgalactosaminidase