Mutation of the RGD sequence does not affect plasma membrane association and growth inhibitory effects of elevated IGFBP-2 in vivo.
Using insulin-like growth factor-binding protein-2 (IGFBP-2) transgenic mice (D mice) as a model of elevated IGFBP-2 expression, which is often found in unphysiological conditions, we found association of IGFBP-2 to purified plasma membranes of many organs. To determine whether the RGD (Arg-Gly-Asp) motif of IGFBP-2 mediates cell surface binding in vivo, we mutated the RGD motif of IGFBP-2 into an RGE (Arg-Gly-Glu) sequence and produced transgenic mice (E mice) which express elevated amounts of mutated IGFBP-2. Our data demonstrate that in vivo IGFBP-2 cell surface association is not dependent on the RGD motif and that mutation of this sequence does not alter growth inhibitory effects of IGFBP-2.
Hoeflich
A
A
Institute of Molecular Animal Breeding, Gene Center, Ludwig-Maximilian University, Feodor-Lynen-Str. 25, 81377, Munich, Germany. hoeflich@lmb.uni-muenchen.de
Reisinger
R
R
Vargas
G A
GA
Elmlinger
M W
MW
Schuett
B
B
Jehle
P M
PM
Renner-Müller
I
I
Lahm
H
H
Russo
V C
VC
Wolf
E
E
D020816:Amino Acid Motifs / Q000235:genetics / Q000502:physiology; D000818:Animals; D001835:Body Weight / Q000235:genetics / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000032:analysis / Q000378:metabolism; D008565:Membrane Proteins / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic / Q000254:growth & development / Q000502:physiology; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism*; D009929:Organ Size / Q000235:genetics / Q000502:physiology; D017354:Point Mutation
D018971:Insulin-Like Growth Factor Binding Protein 2; D008565:Membrane Proteins; D009842:Oligopeptides; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; C047981:arginyl-glycyl-aspartic acid