Bovine embryos contain a higher proportion of polyploid cells in the trophectoderm than in the embryonic disc.
The frequency of polyploid cells in the embryonic disc (ED) and in the trophectoderm (TE) was assessed in 50 in vitro produced bovine embryos fixed at days 7-8 post insemination (pi) and in 20 in vitro produced embryos that were transferred to uteri of recipients at day 7 and then recovered and fixed at day 12 pi. Separation of TE and ED cells was obtained by microdissection and the frequency of polyploid cells was determined by interphase cytogenetic analysis using fluorescence in situ hybridization (FISH) with chromosome 6- and chromosome 7-specific probes. The results show that 96% of day 7 embryos contain polyploid cells in the TE, whereas only 58% contain polyploid cells in the ED. In day 12 embryos 85% of TE and 40% of ED preparations contain polyploid cells. Statistical analysis revealed that the frequency of polyploid cells was significantly higher in the TE than in the ED in embryos containing less than 25% polyploid cells (n = 65). The few embryos (n = 5), which contained more than 25% polyploid cells, did not show this difference. Further, it was revealed that the level of polyploidy on day 7-8 was significantly higher than on day 12, both in the TE (two-fold) and in the ED (seven-fold).
Viuff
Dorthe
D
Department of Clinical Studies, Reproduction, Royal Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark. dv@kvl.dk
Palsgaard
Anne
A
Rickords
Lee
L
Lawson
Lartey G
LG
Greve
Torben
T
Schmidt
Mette
M
Avery
Birthe
B
Hyttel
Poul
P
Thomsen
Preben D
PD
D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002417:Cattle; D004475:Ectoderm / Q000166:cytology; D011123:Polyploidy