Temporal and spatial localization of prothymosin alpha transcript in the Harderian gland of the frog, Rana esculenta.
The Harderian gland (hg) is the only orbital gland of the frog Rana esculenta, and it has the essential function of lubricating the eyes. The hg secretory activity is seasonal, showing the highest value in summer. There is, at present, no data on gene expression of the frog hg. This study reports, for the first time, on the temporal and spatial expression of a cDNA clone encoding for the prothymosin alpha (Prot-alpha), a highly acidic nuclear protein present in virtually all mammalian cells. Northern blot analysis revealed a single 1.7 kb transcript detected in the frog hg throughout the year, with a lowest expression in September in concomitance with the minimum secretory activity. In situ hybridization indicated that hg secretory cells express Prot-alpha transcript, and the hybridization signal was less intense in the September gland. The constant expression of the frog Prot-alpha mRNA during the whole year suggests a constitutive role for this molecule in the hg. In addition, taking into account that, in mammals, many immunomodulatory functions have been attributed to this protein, it is suggested that frog Prot-alpha might contribute to the hg immunity processes, probably acting as a protective agent against infections of the eyeball. Interestingly, although the presence of Prot-alpha gene in animals other than mammals has been considered to be highly unlikely, the present paper confirms the presence of Prot-alpha transcript in a nonmammalian vertebrate, the frog R. esculenta.
De Rienzo
Gianluca
G
Dipartimento di Medicina Sperimentale, Sezione di Fisiologia Umana e Funzioni Biologiche Integrate, F. Bottazzi, Seconda Università degli Studi di Napoli, 80138 Napoli, Italy.
Di Sena
Rona
R
Ferrara
Diana
D
Palmiero
Carmela
C
Chieffi Baccari
Gabriella
G
Minucci
Sergio
S
D000818:Animals; D005786:Gene Expression Regulation; D006243:Harderian Gland / Q000166:cytology / Q000378:metabolism*; D017403:In Situ Hybridization; D009928:Organ Specificity; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011893:Rana esculenta / Q000235:genetics*; D012621:Seasons; D013947:Thymosin / Q000031:analogs & derivatives* / Q000235:genetics*
D011498:Protein Precursors; D012333:RNA, Messenger; C040782:prothymosin alpha; D013947:Thymosin