The mechanism of the angiotensin-converting enzyme inhibitor quinapril is not related to bradykinin level in heart tissue.
In order to examine the effect of the angiotensin-converting enzyme inhibitor (ACEi) quinapril, we performed a sensitive and specific radioimmunoassay (RIA) to quantify bradykinin, BK-(1-9), in heart and kidney tissues. The BK-(1-9) level was unaffected in the heart of sham and water-deprived rats treated for 2h with quinapril (10mg/kg), but was significantly higher in the kidneys in the two groups. In these conditions, circulating and tissue angiotensin II (Ang II) levels were significantly decreased by quinapril. Moreover, our results indicated that acute treatment with this dose of quinapril induced kinin-mediated effects which were not related to its action on bradykinin degradation in rat hearts.
Barthelemy
Christiane
C
Laboratoire de Biochimie médicale, IFR 14, CHU Pitié-Salpêtrière, 91 Bd de l'Hopital, 75634 Cedex 13, Paris, France. barthele@ccr.jussieu.fr
Eurin
Joelle
J
Lechat
Philippe
P
Masson
Francoise
F
Cortines
Micheline
M
Mougenot
Nathalie
N
Soualmia
Hayet
H
Carayon
Alain
A
D000803:Angiotensin I / Q000096:biosynthesis / Q000097:blood; D000804:Angiotensin II / Q000096:biosynthesis / Q000097:blood; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D001920:Bradykinin / Q000096:biosynthesis*; D007546:Isoquinolines / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D000077583:Quinapril; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044005:Tetrahydroisoquinolines; D013997:Time Factors; D014018:Tissue Distribution
D000806:Angiotensin-Converting Enzyme Inhibitors; D007546:Isoquinolines; D044005:Tetrahydroisoquinolines; D000804:Angiotensin II; D000803:Angiotensin I; D007703:Peptidyl-Dipeptidase A; D000077583:Quinapril; D001920:Bradykinin