Murine peripherin gene sequences direct Cre recombinase expression to peripheral neurons in transgenic mice.
Spatially and temporally regulated somatic mutations can be achieved by using the Cre/loxP recombination system of bacteriophage P1. To develop a cell type-specific system of gene targeting in the peripheral nervous system, we generated the transgenic mouse lines expressing Cre recombinase under the control of the mouse peripherin gene promoter. The activity of the Cre recombinase during embryonic development was examined by mating the peripherin-Cre transgenic mice to the knock-in Cre-mediated recombination reporter strain, R26R. Analysis of F1 embryos from this cross showed specific excision of loxP-flanked sequences in the dorsal root ganglia, trigeminal ganglia, and olfactory epithelium, in a pattern very similar to the expression of the endogenous mouse peripherin gene, and the previously reported peripherin-lacZ transgenic mice. Thus, the peripherin-Cre mouse described here will provide a valuable tool for Cre-loxP-mediated conditional expression in the peripheral nervous system.
Transgenic and Knockout Facility Section, Gerontology Research Center, IRP, NIA, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224-6825, USA.
Li
L
Zhou
Virginie
V
Népote
Daniel L
DL
Rowley
Béatrice
B
Levacher
Agnes
A
Zvara
Miklos
M
Santha
Qing Sheng
QS
Mi
Michel
M
Simonneau
David M
DM
Donovan
D000818:Animals; D005727:Ganglia, Spinal / Q000378:metabolism*; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000235:genetics*; D008562:Membrane Glycoproteins; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009831:Olfactory Mucosa / Q000378:metabolism*; D064531:Peripherins; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics; D022661:RNA, Untranslated; D019076:Transgenes / Q000235:genetics / Q000502:physiology; D012668:Trigeminal Ganglion / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D064531:Peripherins; D011506:Proteins; D022661:RNA, Untranslated; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases