Nef enhances human immunodeficiency virus type 1 infectivity and replication independently of viral coreceptor tropism.
We investigated the infectivities and replicative capacities of a large panel of variants of the molecular human immunodeficiency virus type 1 (HIV-1) NL4-3 clone that differ exclusively in the V3 region of the viral envelope glycoprotein and the nef gene. Our results demonstrate that Nef enhances virion infectivity and HIV-1 replication independently of the viral coreceptor tropism.
Institute for Clinical and Molecular Virology, University of Erlangen-Nürnberg, Germany.
Armin
A
Papkalla
Jan
J
Münch
Claas
C
Otto
Frank
F
Kirchhoff
D000595:Amino Acid Sequence; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D015754:Genes, nef; D014644:Genetic Variation; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D019713:Receptors, CCR5 / Q000502:physiology; D019718:Receptors, CXCR4 / Q000502:physiology; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid; D014774:Virulence / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D019713:Receptors, CCR5; D019718:Receptors, CXCR4