Immobilization of invertase on rice husk using polyethylenimine.
Washed and dried rice husk was coated with 2% polyethylenimine (PEI). Invertase was immobilized on this support through adsorption followed by cross-linking with 2% glutaraldehyde. Immobilized enzyme was reused for the hydrolysis of sucrose without loss in activity. This approach may serve as a simple technique in the future for the covalent immobilization of enzymes on lignocellulosic supports.
D'Souza
S F
SF
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. sfdsouza@apsara.barc.ernet.in
Godbole
S S
SS
D000327:Adsorption; D004800:Enzymes, Immobilized; D005976:Glutaral / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006868:Hydrolysis; D012275:Oryza / Q000201:enzymology*; D011094:Polyethyleneimine / Q000737:chemistry*; D013395:Sucrose / Q000737:chemistry; D043324:beta-Fructofuranosidase
D004800:Enzymes, Immobilized; D013395:Sucrose; D011094:Polyethyleneimine; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D005976:Glutaral