Behaviour of vanadate and vanadium-transferrin complex on different anion-exchange columns. Application to in vivo 48V-labelled rat serum.
The behaviour of free [48V]vanadate and [48V]vanadium-transferrin complex was investigated on five different anion-exchange columns (Mono Q 5/5 HR, Hitrap Q HP, Sepharose Q FF, Sepharose DEAE FF and Hitrap Q XL). The recovery of both V-compounds was quantitative. The peak shape and retention time of vanadate varied according to the type of column. The vanadium-transferrin complex also showed different elution patterns depending on the type of column. Especially in case of the Sepharose Q FF, Mono Q 5/5 HR and Hitrap Q XL columns the vanadium-transferrin binding was degraded during elution on the column. The results clearly prove that care should be taken as to the choice of column for speciation purposes of vanadium compounds in order to prevent various artefacts showing up in the chromatograms. A Hitrap Q HP column was used to fractionate different vanadium compounds in rat serum.
De Cremer
Koen
K
Laboratory for Analytical Chemistry, Institute of Nuclear Sciences, Ghent University, Proeftuinstraat 86, B-9000 Ghent, Belgium. koen.decremer@rug.ac.be
Cornelis
Rita
R
Strijckmans
Karel
K
Dams
Richard
R
Lameire
Norbert
N
Vanholder
Raymond
R
D000818:Animals; D000837:Anion Exchange Resins; D002852:Chromatography, Ion Exchange / Q000295:instrumentation; D051381:Rats; D014168:Transferrin / Q000737:chemistry*; D014638:Vanadates / Q000737:chemistry*; D014639:Vanadium / Q000737:chemistry*
D000837:Anion Exchange Resins; D014168:Transferrin; D014639:Vanadium; D014638:Vanadates