Molecular sexing of monomorphic endangered Ara birds.
Survival of most endangered birds may depend on breeding programs where sex identification plays an important role. Molecular sexing has shown to be a rapid and safe procedure. In this work we established sex identification of monomorphic endangered Ara birds using a chromosome W-linked DNA marker, the Chromo-helicase-DNA-Binding 1 (CHD) gene. Most birds have two CHD sex-linked genes, one W-linked (CHD-W) and one Z-linked (CHD-Z). These markers were characterized from Ara militaris and gender sex was determined by PCR and restriction analyzes. The procedure here reported was successfully applied to five different species of the genus Ara and confirmed the validity of the technique. To our knowledge, this is the first report of molecular sexing of the Ara species. This molecular sexing is currently been used in breeding programs of Ara birds.
Bermúdez-Humarán
Luis Gilberto
LG
Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas. Universidad Autónoma de Nuevo León, San Nicolás de los Garza, N.L. México.
García-García
Aracely
A
Leal-Garza
Carlos Humberto
CH
Riojas-Valdes
Victor Manuel
VM
Jaramillo-Rangel
Gilberto
G
Montes-de-Oca-Luna
Roberto
R
D000818:Animals; D001483:Base Sequence; D001947:Breeding / Q000379:methods*; D003247:Conservation of Natural Resources; D004268:DNA-Binding Proteins / Q000235:genetics; D017753:Ecosystem; D005260:Female; D008040:Genetic Linkage / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction; D011563:Psittaciformes / Q000235:genetics*; D015203:Reproducibility of Results; D015183:Restriction Mapping; D012730:Sex Chromosomes / Q000235:genetics*; D012732:Sex Determination Analysis / Q000379:methods* / Q000662:veterinary*; D013045:Species Specificity
D004268:DNA-Binding Proteins; D005819:Genetic Markers