RNA folding in vivo.
RNA folding in vivo is influenced by the cellular environment, the vectorial nature of transcription and translation, trans-acting factors and ion homeostasis. Specific RNA-binding proteins promote RNA folding by stabilizing the native structure or by guiding folding. In contrast, RNA chaperones, which are believed to interact nonspecifically with RNA, were proposed to resolve misfolded RNA structures and to promote intermolecular RNA-RNA annealing. Small trans-acting noncoding RNAs are thought to modulate mRNA structures, thereby regulating gene expression. So far, there is some evidence that in vitro and invivo RNA folding pathways share basic features. However, it is unclear whether the rules deduced from in vitro folding experiments generally apply to invivo conditions.
Institute of Microbiology and Genetics, University of Vienna, Vienna Biocenter, Doktor Bohrgasse 9, A-1030 Vienna, Austria. renee@gem.univie.ac.at
Renée
R
Schroeder
Rupert
R
Grossberger
Andrea
A
Pichler
Christina
C
Waldsich
D024509:3' Flanking Region; D024506:5' Flanking Region; D000818:Animals; D006706:Homeostasis; D007477:Ions / Q000378:metabolism; D008322:Mammals / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D013768:Tetrahymena / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D007477:Ions; D018832:Molecular Chaperones; D016601:RNA-Binding Proteins; D012313:RNA