Effectiveness of chemical sanitizers against Campylobacter jejuni-containing biofilms.
Survival of Campylobacter jejuni in mixed-culture biofilms was determined after treatment with chemical sanitizers including chlorine, quaternary ammonia, peracetic acid (PAA), and a PAA/peroctanoic acid mixture (PAA/POA). Biofilm-producing bacteria (gram-positive rods, Y1 and W1) were isolated from chicken house nipple drinkers. A meat plant isolate (Pseudomonas sp.) was also included as a biofilm producer. Two-day-old biofilms grown on polyvinyl chloride (PVC) plastic coupons in R2A broth at 12 degrees C were incubated with 10(6) CFU/ml C jejuni for 6 h to allow attachment. The coupons were then rinsed and incubated in fresh media for an additional 24 h. C. jejuni-containing biofilms were detached by vortexing with glass beads in modified brucella broth, which was then enumerated for C. jejuni on selective/differential media. The presence of biofilm enhanced (P < 0.01) the attachment and survival of C. jejuni After the 24-h incubation, only 20 CFU/cm2 of C. jejuni were recovered from the control without biofilms compared to 2,500 to 5,000 CFU/cm2 in samples with preexisting biofilms. The presence of biofilm microflora decreased (P < 0.01) the effectiveness of sanitizers against C. jejuni. Chlorine was the most effective sanitizer since it completely inactivated C. jejuni in the biofilms after treatment at 50 ppm for 45 s. C. jejuni in biofilms was susceptible to all sanitizers tested but was not completely inactivated by treatment with quaternary ammonia, PAA, or PAA/POA mixture at 50 and 200 ppm for 45 s.
Center for Food Safety, Department of Food Science and Technology, University of Georgia, Athens 30602-2106, USA.
Nathanon
N
Trachoo
Joseph F
JF
Frank
D000641:Ammonia / Q000494:pharmacology; D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000502:physiology*; D002713:Chlorine / Q000494:pharmacology; D015169:Colony Count, Microbial; D003470:Culture Media; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D010463:Peracetic Acid / Q000494:pharmacology; D013997:Time Factors; D016896:Treatment Outcome
D003470:Culture Media; D004202:Disinfectants; D002713:Chlorine; D000641:Ammonia; D010463:Peracetic Acid