Production of lipolyzed butteroil by a calf pregastric esterase immobilized in a hollow fiber reactor: III. Effect of glycerol.
Lipolysis of butter oil in a hollow fiber reactor containing an immobilized calf pregastric esterase was studied at 40 degrees C, a pH of 6.0, and glycerol concentrations of 0, 150, and 500 g/L in the buffer solution. The concentrations of 10 fatty acid species in the lipolyzed product were determined using high-performance liquid chromatography. The rate of loss of enzyme activity and the relative selectivities of this esterase depended on the glycerol concentration. By contrast, the overall rate of release of fatty acids was not affected by the glycerol concentration. Loss of enzyme activity was modeled using first-order kinetics. The models for deactivation and reaction kinetics were fit simultaneously to the data. The model was successful in describing the rates of release of all 10 fatty acid species for a range of space times from 0 to 25 h. The parameters of the model were tested for dependence on glycerol concentration.
Department of Chemical Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, 53706, USA.
Louis P
LP
Lessard
Charles G
CG
Hill
D000818:Animals; D019149:Bioreactors; D002079:Butter; D003198:Computer Simulation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D005227:Fatty Acids / Q000138:chemical synthesis*; D005524:Food Technology; D005990:Glycerol / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D008956:Models, Chemical; D012043:Regression, Psychology; D012680:Sensitivity and Specificity; D012756:Sheep
D004800:Enzymes, Immobilized; D005227:Fatty Acids; D002079:Butter; D008049:Lipase; C043101:gastric triacylglycerol lipase; D005990:Glycerol