Proliferating cell nuclear antigen positive cells in the hippocampal subgranular zone decline after irradiation in a rodent model.
Four-week-old ICR mice were systemically exposed to 18Gy X-rays. Afterwards, the expression of proliferating cell nuclear antigen (PCNA) in the cerebrum was observed with time using Western blot analysis and immunohistochemical staining. As a result, PCNA-positive cells were observed in the subgranular zone (SGZ) of the hippocampus and subventricular zone (SVZ) of the lateral ventricles in unirradiated mice. The number of PCNA-positive cells decreased with time in all zones after irradiation, but the decrease was more marked in the hippocampal SGZ. We think that PCNA-positive cells are stem cells. The selective vulnerability to radiation in the hippocampus is considered to be attributed to the fact that stem cells in the SGZ selectively undergo radiation-induced apoptosis.
Laboratory of Neuropathology, Graduate School of Science, Osaka Prefecture University, Sakai, Japan.
R
R
Nagai
S
S
Tsunoda
H
H
Asada
N
N
Urabe
D000818:Animals; D017209:Apoptosis / Q000502:physiology / Q000528:radiation effects*; D002455:Cell Division / Q000502:physiology / Q000528:radiation effects*; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000502:physiology / Q000528:radiation effects; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D007150:Immunohistochemistry; D020547:Lateral Ventricles / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism / Q000528:radiation effects*; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D013997:Time Factors; D014965:X-Rays / Q000009:adverse effects*
D018809:Proliferating Cell Nuclear Antigen