Supplementation of heterologous complement induces anti-Thy-1.1 nephritis in the Mongolian gerbil (Meriones unguiculatus).
Anti-Thy-1.1 nephritis in the rat is a popular experimental model for mesangial proliferative glomerulonephritis (GN). This model is characterized by direct binding of anti-Thy-1.1 antibody with Thy-1.1 antigen expressed on mesangial cells (MCs) of glomeruli in the rat. A single injection of anti-rat thymocyte serum (ARTS) results in GN with proteinuria and extensive mesangiolysis. Development of mesangiolysis and proteinuria are complement-dependent. We previously demonstrated Thy-1.1 antigen, similar to the rat, in thymocytes, brain cells and MCs of the kidney in the Mongolian gerbil (MG). In this study, we attempted to develop a MG nephritis model, but an injection of ARTS did not induce GN. An additional injection of guinea pig serum as a complement after ARTS injection resulted in anti-Thy-1.1 nephritis in MG. Degeneration of MCs and neutrophil infiltration were observed 1 hr after GP serum injection. Mesangiolysis and fibrin exudation occurred 12 hr after the injection and MC proliferation was apparent 7 days after the injection. In the complement-dependent hemolytic test, MG serum could not hemolyze sheep erythrocytes. These results suggested low activity, or depletion of some factors, in complements of MG serum.
Shichinohe
Kazuhiro
K
Department of Laboratory Animal Science, Nippon Medical School, Tokyo, Japan.
Shimizu-Suganuma
Masumi
M
Ghazizadeh
Mohammad
M
Ishizaki
Masamichi
M
D000818:Animals; D003165:Complement System Proteins / Q000494:pharmacology*; D004195:Disease Models, Animal; D005849:Gerbillinae / Q000276:immunology*; D015432:Glomerulonephritis, Membranoproliferative / Q000276:immunology* / Q000473:pathology / Q000662:veterinary*; D006168:Guinea Pigs; D006461:Hemolysis; D007150:Immunohistochemistry / Q000662:veterinary; D007668:Kidney / Q000473:pathology*; D008297:Male; D008854:Microscopy, Electron / Q000662:veterinary; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D018800:Thy-1 Antigens / Q000276:immunology*
D018800:Thy-1 Antigens; D003165:Complement System Proteins