Bone colonization of beta-TCP granules incorporated in brushite cements.
Injectable calcium phosphate hydraulic cements are known to have a high clinical potential in bone reconstruction for mini-invasive orthopaedic surgery, interventional radiology, and rheumatology. Previous in vivo experiments in rabbit have shown that the presence of beta-TCP granules in injectable bone cement help maintain the transient biomechanical function of the implanted bone and promote the formation of good-quality new bone. Histomorphometric analysis of two brushite hydraulic cement (BHC) mixtures selected from previous results (referred to in this work as BHC-A and BHC-B) was performed at three postoperative delays (0, 12, and 24 weeks): histomorphometric analysis of bone colonization within beta-TCP shows that, just before implantation, the beta-TCP granule area is significantly higher in BHC-B; the residual granule area decreases steadily over time in BHC-A, whereas it goes through a maximum of 30% at 12 weeks in BHC-B; the residual granule porosity increases steadily up to 35% in BHC-A, whereas it goes through a maximum of 35% at 12 weeks and decreases somewhat until 24 weeks in BHC-B. New bone formation within granules appears higher in BHC-A (58% Area) compared to BHC-B (38% area) at 12 weeks. At 24 weeks bone colonization levels off in both cements at about 50% area. Irrespective of the cement matrix composition, beta-TCP granules contribute actively to the conduction of new bone formation.
IR2B, Université du Littoral, 52 rue du Docteur Calot, F-62608 Berck-sur-Mer Cedex, France.
B
B
Flautre
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Maynou
J
J
Lemaitre
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Van Landuyt
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P
Hardouin
D000818:Animals; D001843:Bone Cements / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D001847:Bone Diseases / Q000601:surgery / Q000628:therapy; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D010012:Osteogenesis / Q000187:drug effects*; D016062:Porosity; D011817:Rabbits; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods
D001843:Bone Cements; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous