[Use of specific antibodies labelled with colloid silver particles for detection of Brucella antigens with dot immune analysis].
Fitness of dot immuno-analysis for detection of Brucella antigens labeled with colloid silver is evaluated. Soluble lipopolysaccharides and protein-saccharide antigen and corpuscular antigens of 22 Brucella strains (7 species) pathogenic for humans and animals in the S and R forms were used. The specificity of the method was tested on 10 heterologous microorganisms whose antigens were closely related. The suggested test system is simple, economic, highly sensitive (from 62 thousands to 8 million CFU/ml) and specific, requires no expensive equipment, and is an alternative to enzyme immuno-assay and dot immuno-analysis with gold immunosole.
T Iu
TIu
Zagoskina
A I
AI
Kalinovskiĭ
E Iu
EIu
Markov
A A
AA
Dokorina
E P
EP
Golubinskiĭ
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis*; D002002:Brucella / Q000276:immunology*; D003102:Colloids; D015151:Immunoblotting; D011817:Rabbits; D012834:Silver
D000906:Antibodies; D000942:Antigens, Bacterial; D003102:Colloids; D012834:Silver