Modeling both the mechanical and hypoxic features of traumatic brain injury in vitro in rats.
In traumatic brain injury, the brain is subjected to mechanical shear and varying degrees of hypoxia/ischemia. To compare effects of stretch injury, hypoxia, and the combination of both insults on neurons, mixed neuronal and astrocytic cultures were established from day 17 fetal rat brains. On days 17-19 in vitro, cultures were subjected to stretch injury or hypoxia of varying degrees, alone and in combination. Cultures were assayed for lactate dehydrogenase release and Trypan Blue uptake. Hypoxia or Stretch injury alone induced a graded response (P<0.05) on both assays. Stretch+Hypoxia (4 or 6 h) resulted in significantly greater injury as compared with controls (P<0.05), and as compared with either isolated Stretch or Hypoxia (1, 2, 4 or 6 h) alone (P<0.05).
Department of Pediatric Emergency Medicine (Location C 2008), Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA. glast0@chmcc.org
Todd F
TF
Glass
Brandi
B
Reeves
Frank R
FR
Sharp
D000818:Animals; D001696:Biomechanical Phenomena; D001921:Brain / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D001930:Brain Injuries / Q000201:enzymology* / Q000503:physiopathology; D018929:Cell Culture Techniques / Q000379:methods*; D016923:Cell Death / Q000502:physiology; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D005333:Fetus; D002534:Hypoxia, Brain / Q000201:enzymology* / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008567:Membranes, Artificial; D016501:Neurites / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D010812:Physical Stimulation; D011247:Pregnancy; D051381:Rats; D013718:Tensile Strength / Q000502:physiology; D014343:Trypan Blue / Q000494:pharmacology
D004396:Coloring Agents; D008567:Membranes, Artificial; D007770:L-Lactate Dehydrogenase; D014343:Trypan Blue