Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis.
A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.
Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, Antwerp 2000, Belgium. palomino@itg.be
Juan-Carlos
JC
Palomino
Anandi
A
Martin
Mirtha
M
Camacho
Humberto
H
Guerra
Jean
J
Swings
Françoise
F
Portaels
D000995:Antitubercular Agents / Q000494:pharmacology*; D003124:Colorimetry; D004352:Drug Resistance, Microbial; D006801:Humans; D007202:Indicators and Reagents; D007538:Isoniazid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D010078:Oxazines; D010568:Peru; D012293:Rifampin / Q000494:pharmacology; D014376:Tuberculosis / Q000382:microbiology; D014966:Xanthenes
D000995:Antitubercular Agents; D007202:Indicators and Reagents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D007538:Isoniazid; D012293:Rifampin