Biosynthetic study of amphidinolide W.
The biosynthetic origins of amphidinolide W (1) were investigated on the basis of (13)C-NMR data of 13C-enriched samples obtained by feeding experiments with [1-13C], [2-13C], and [1,2-13C2] sodium acetate in cultures of a strain Y-42 of the dinoflagellate Amphidinium sp. These incorporation patterns suggested that 1 was generated from a hexaketide chain, two acetate units, four isolated C1 units from C-2 of acetates, and four branched C1 units from C-2 of acetates. The acetate-incorporation patterns for C-1-C-2-(C-21) and C-8-C-18-(C-23, C-24) of 1 corresponded well to those for C-1-C-2-(C-27) and C-5-C-15-(C-28, C-29) of amphidinolide H (2) isolated from this strain.
Tsuda
Masashi
M
Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Izui
Naoko
N
Sato
Masaaki
M
Kobayashi
Jun'ichi
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004141:Dinoflagellida / Q000737:chemistry / Q000378:metabolism*; D018942:Macrolides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D019346:Sodium Acetate / Q000378:metabolism; D000097442:Amphidinolides
D003470:Culture Media; D018942:Macrolides; D019346:Sodium Acetate; C449925:amphidinolide W; D000097442:Amphidinolides