Laminin-5 is targeted by autoantibodies in feline mucous membrane (cicatricial) pemphigoid.
In human and canine patients with mucous membrane (cicatricial) pemphigoid (MMP), circulating autoantibodies have been shown to target multiple epidermal basement membrane antigenic epitopes. These autoantigens include collagen XVII in humans and dogs, as well as laminin-5, laminin-6 or integrin alpha-6/beta-4 in human beings. The purpose of this study was to determine if autoantibodies targeted laminin-5 in a cat exhibiting clinical and microscopic lesions resembling those of MMP in humans. In this patient, an indirect immunofluorescence (IF) assay revealed circulating IgG and IgA autoantibodies that bound to the basement membrane zone on the dermal side of salt-split gingiva (titer 1:1000 for IgG and 1:50 for IgA). Immunoblotting, performed with affinity-purified human laminin-5, demonstrated that the autoantibodies bound the alpha-3 chain of this heterotrimer. These observations identify laminin-5 as one of the antigens recognized by circulating autoantibodies in this feline homologue of MMP in humans and dogs.
Olivry
Thierry
T
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC, USA. thierry_olivry@ncsu.edu
Dunston
Stanley M
SM
Zhang
Guyiun
G
Ghohestani
Reza F
RF
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology; D002371:Cat Diseases / Q000276:immunology*; D002415:Cats / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008856:Microscopy, Fluorescence; D010390:Pemphigoid, Benign Mucous Membrane / Q000276:immunology* / Q000662:veterinary*; D000097794:Kalinin
D001323:Autoantibodies; D015815:Cell Adhesion Molecules; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D000097794:Kalinin