Hepatic ischemia-reperfusion promotes liver metastasis of colon cancer.
BACKGROUND
The effects of hepatic ischemia/reperfusion (I/R) on liver metastasis have not been fully examined. We examined hepatic I/R and liver metastasis of colorectal cancer in a rat model; we also quantitated expression of E-selectin (ELAM-1) mRNA after I/R.


METHODS
Rats underwent 30 or 60 min of 70% partial hepatic ischemia. After 60 min of reperfusion, rat colon adenocarcinoma cells (RCN-H4) were inoculated intrasplenically. The number of tumor nodules on the liver surface was determined 3 weeks later. Expression of E-selectin mRNA was determined at 1, 3, and 6 h after ischemia by quantitative RT-PCR.


RESULTS
Hepatic I/R promoted liver metastasis of RCN-H4 and induced the expression of E-selectin mRNA in both clamped and unclamped liver lobes. The number of tumor nodules and the expression of E-selectin mRNA after 60 min of ischemia was greater than that after 30 min.


CONCLUSIONS
Hepatic I/R, especially with a long duration of ischemia, induces expression of E-selectin and promotes liver metastasis of colon cancer in rats.
Second Department of Surgery, Fukui Medical University, Fukui, Japan. kodo@fki.fitweb.or.jp
Koji
K
Doi
Tetsuya
T
Horiuchi
Masaru
M
Uchinami
Takanori
T
Tabo
Narisato
N
Kimura
Jun
J
Yokomachi
Makoto
M
Yoshida
Kuniyoshi
K
Tanaka
D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology*; D019040:E-Selectin / Q000235:genetics; D007511:Ischemia / Q000150:complications*; D008099:Liver / Q000378:metabolism; D008102:Liver Circulation; D008113:Liver Neoplasms / Q000209:etiology* / Q000556:secondary*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D015427:Reperfusion Injury / Q000150:complications*
D019040:E-Selectin; D012333:RNA, Messenger