Isolation of a novel peroxidase from French bean legumes and first demonstration of antifungal activity of a non-milk peroxidase.
A novel antifungal protein with its N-terminal sequence bearing similarity to the C-terminal sequences of peroxidases was isolated from French bean legumes. The protein, which possessed a molecular weight of 37 kDa, was adsorbed on Affi-gel blue gel and CM-Sepharose. The protein exhibited peroxidase activity with a Km of 58 microM and a Vmax of 3.36 U/nmol. Optimal peroxidase activity was found at 22 degrees C and pH 4. It exerted antifungal activity against a variety of fungal species including Coprinus comatus, Mycosphaerella arachidicola, Fusarium oxysporum and Botrytis cinerea. It inhibited the activities of alpha-glucosidase and beta-glucosidase but was without any inhibitory effect on HIV-1 reverse transcriptase.
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Department of Biochemistry, Faculty of Medicine, The Chinese, University of Hong Kong, Shatin, China.
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D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005658:Fungi / Q000187:drug effects; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D007700:Kinetics; D007784:Lactoperoxidase / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010544:Peroxidases / Q000302:isolation & purification / Q000494:pharmacology*; D027805:Phaseolus / Q000201:enzymology*; D014176:Protein Biosynthesis / Q000187:drug effects; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
D000935:Antifungal Agents; D018894:Reverse Transcriptase Inhibitors; D007784:Lactoperoxidase; D010544:Peroxidases; D054303:HIV Reverse Transcriptase; D006026:Glycoside Hydrolases