Ultrastructural cytochemical observations of the cell coat of two Trichomonas vaginalis isolates with different degree of virulence.
The cell coat of two Trichomonas vaginalis isolates with different degree of virulence isolated in Mexico from symptomatic women was studied by cytochemical assays. The use of carbohydrate cell surface markers allowed us to visualize greater electron-dense deposits in the highly virulent T. vaginalis isolate than in the less virulent one. On the contrary, parasites treated with concanavalin A showed a heavy uniform electron-dense deposit on the cell surface that was similar in both isolates. When parasites were treated with cationized ferritin the amount of bounded particles on the cell surface was greater in the higher virulent isolate.
González-Robles
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A
Department of Experimental Pathology, CINVESTAV, IPN, Mexico, DF, Mexico. goroa@mail.cinvestav.mx
Cristóbal-Ramos
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AR
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D019276:Glycocalyx / Q000737:chemistry / Q000648:ultrastructure*; D006651:Histocytochemistry; D006790:Host-Parasite Interactions; D013194:Staining and Labeling; D014246:Trichomonas vaginalis / Q000737:chemistry / Q000472:pathogenicity / Q000648:ultrastructure*; D037521:Virulence Factors
D002241:Carbohydrates; D037521:Virulence Factors