Amino acid sequence of the major form of toad liver glutathione transferase.
To investigate structural relationship between amphibian and mammalian GSTs the complete amino acid sequence of the major form of glutathione transferase present in toad liver (Bufo bufo) was determined. The enzyme subunit is composed of 210 amino acid residues corresponding to a molecular mass of 24,178 Da. In comparison with the primary structure of amphibian bbGSTP1-1, toad liver GST showed 54% sequence identity. On the other hand, toad liver GST showed about 45-55% sequence identity when compared with other pi class GST and less then 25% identity with GST of other classes. Amino acid residues involved in the H site and in the key and lock structure of the toad enzyme are significantly different from those of bbGSTP1-1 and other mammalian pi class GST. On the basis of its structural and immunological properties the toad liver GST, indicated as bbGSTP2-2, could represent the prototype of a subset of the pi family.
Bucciarelli
Tonino
T
Dipartimento di Scienze Biomediche, Università G. D'Annunzio, Chieti, Italy.
Sacchetta
Paolo
P
Amicarelli
Fernanda
F
Petruzzelli
Raffaele
R
Melino
Sonia
S
Rotilio
Domenico
D
Celli
Nicola
N
Di Ilio
Carmine
C
D000595:Amino Acid Sequence; D000818:Animals; D002023:Bufo bufo; D005982:Glutathione Transferase / Q000737:chemistry*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D005982:Glutathione Transferase