Brn-1 and Brn-2 share crucial roles in the production and positioning of mouse neocortical neurons.
Formation of highly organized neocortical structure depends on the production and correct placement of the appropriate number and types of neurons. POU homeodomain proteins Brn-1 and Brn-2 are coexpressed in the developing neocortex, both in the late precursor cells and in the migrating neurons. Here we show that double disruption of both Brn-1 and Brn-2 genes in mice leads to abnormal formation of the neocortex with dramatically reduced production of layer IV-II neurons and defective migration of neurons unable to express mDab1. These data indicate that Brn-1 and Brn-2 share roles in the production and positioning of neocortical neuron development.
Department of Cell Biology, JFCR-Cancer Institute, Tokyo 170-8455, Japan.
Yoshinobu
Y
Sugitani
Shigeyasu
S
Nakai
Osamu
O
Minowa
Miyuki
M
Nishi
Kou-Ichi
K
Jishage
Hitoshi
H
Kawano
Kensaku
K
Mori
Masaharu
M
Ogawa
Tetsuo
T
Noda
D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D019070:Cell Lineage; D005314:Embryonic and Fetal Development; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D019579:Neocortex / Q000166:cytology* / Q000196:embryology; D009419:Nerve Tissue Proteins / Q000235:genetics; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics / Q000502:physiology*; D050809:POU Domain Factors; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
D015415:Biomarkers; C103773:Dab1 protein, mouse; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D050809:POU Domain Factors; D015534:Trans-Activators; D014157:Transcription Factors; C085076:transcription factor Brn-2; C074065:Pou3f3 protein, mouse