[BRCA2 gene mutation detection in hereditary ovarian cancer tissues].
OBJECTIVE
The aim of this study was to detect the mutation of BRCA2 gene in hereditary ovarian cancer tissues to probe the clinical significance of it.


METHODS
DNA was abstract from paraffin embedded tissues archived in pathology department. A pair of primer located on 11 exon was used to detect the 6174 delT mutation,BRCA2 gene mutation in 12 hereditary ovarian cancer patients and 15 sporadic ovarian cancer patients were screened by polymerase chain reaction-single strand conformation polymorphism analysis with DNA non- isotopic silver staining methods.


RESULTS
Two of 12 hereditary ovarian cancer victims were found carrying mutation of BRCA2 gene. The mutation type was 6174del T in the 11 exon of BRCA2 gene. No mutation of BRCA2 gene on this site was found in 15 sporadic ovarian cancer patients.


CONCLUSIONS
BRCA2 gene mutation was closely associated with the carcinogenesis and development of hereditary ovarian cancer, but had no relationship with sporadic ovarian cancer.
Department of Obstetrics and Gynecology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052,China.
Yuhuan
Y
Qiao
Ping
P
Chen
Huirong
H
Shi
Guoqiang
G
Zhao
Dan
D
Wu
D005260:Female; D024522:Genes, BRCA2; D005820:Genetic Testing; D006801:Humans; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000517:prevention & control; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational