Interaction process between ionic surfactant and protein probed by series piezoelectric quartz crystal technique.
A method for probing the interaction process between ionic surfactant and protein was developed with series piezoelectric quartz crystal (SPQC) sensing technique. It was based on the sensitive response of the SPQC sensor to the change in solution conductivity. A new relationship between the sensor response and the properties of ionic species in solution was derived. The method was used to examine the interaction process of two surfactants, cetyltrimethylammonium bromide (CTAB) and sodium dodecyl sulfate (SDS), with lysozyme in aqueous solution. The obtained experimental results were in agreement with those of other methods from references. These results had been discussed. It was shown that the new method developed here was a useful and promising tool for probing the ionic surfactant-protein interaction process and might find more applications in similar studies.
Mao
Youan
Y
College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. youanmao@cmmail.com
Wei
Wanzhi
W
Zhang
Jinzhong
J
Zhang
Shufen
S
D001703:Biophysics / Q000379:methods; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D007477:Ions; D015233:Models, Statistical; D009113:Muramidase / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins; D011791:Quartz; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D013997:Time Factors
D002593:Cetrimonium Compounds; D007477:Ions; D011506:Proteins; D013501:Surface-Active Agents; D011791:Quartz; D012967:Sodium Dodecyl Sulfate; D009113:Muramidase; D000077286:Cetrimonium