Glucose and Insulin Regulate Leptin Expression in 3T3-F442A Adipocytes.
Relative quantitation RT-PCR was used to investigate the regulation of leptin expression in 3T3-F442A adipocytes by glucose and insulin. The results showed that glucose and insulin stimulated the expression of leptin in 3T3-F442A adipocytes, but they did not act synergically. Over-high concentration of glucose suppressed the expression of leptin and the effect of insulin. The elevation of the expression of leptin was characterized with saturation. The concentration of glucose is very important for the regulation of leptin expression.
The Laboratory of Molecular Genetics, Shanghai Medical University, Shanghai 200032, China. hysong@shmu.edu.cn
Fang-Nian
FN
Wang
Chun-Gu
CG
Ma
Nai-Xia
NX
Zhang
Hou-Yan
HY
Song