Enterohemolysin operon of Shiga toxin-producing Escherichia coli: a virulence function of inflammatory cytokine production from human monocytes.
Shiga toxin-producing Escherichia coli (STEC) is associated with hemolytic uremic syndrome (HUS). Although most clinical isolates of STEC produce hemolysin (called enterohemolysin), the precise role of enterohemolysin in the pathogenesis of STEC infections is unknown. Here we demonstrated that E. coli carrying the cloned enterohemolysin operon (hlyC, A, B, D genes) from an STEC human strain induced the production of interleukin-1beta (IL-1beta) through its mRNA expression but not tumor necrosis factor-alpha from human monocytes. No IL-1beta release was observed with an enterohemolysin (HlyA)-negative, isogenic E. coli strain carrying a mutation in the hlyA gene. The data suggest that enterohemolysin, a pore-forming toxin, induces the production of IL-1beta, which is one of serum risk markers for HUS.
Taneike
Ikue
I
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, 757 Ichibanchou, Asahimachidori, Niigata, Japan.
Zhang
Hui-Min
HM
Wakisaka-Saito
Noriko
N
Yamamoto
Tatsuo
T
D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000235:genetics*; D006461:Hemolysis; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics; D009000:Monocytes / Q000378:metabolism* / Q000382:microbiology; D009876:Operon; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D022621:Shiga Toxin / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics; D014774:Virulence
D017931:DNA Primers; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C066219:enterohemolysin; D022621:Shiga Toxin