Hormonal measurements in late pregnancy and parturition in dairy cows--possible tools to monitor foetal well being.
Three dairy heifers (A, B and C) were induced to parturition with two prostaglandin (PG) F(2alpha) injections on day 268 and 269 of pregnancy. Signs of approaching parturition were carefully observed. The following parameters were registered: degrees of calving difficulty, date and time of parturition, calf's birth weight and calf's sex. Body temperature was measured and blood samples were taken every 3 h 3 days before the first PGF(2alpha) injection until 3 days after parturition. The plasma concentrations of the PGF(2alpha) metabolite, progesterone, cortisol, oestrone sulphate and pregnancy associated glycoproteins (PAGs) were analysed. Heifers A, B and C delivered 48, 51 and 57 h after the first PGF(2alpha) injection, respectively. Heifer A delivered without any signs of calving difficulty, whereas, the parturition was considered to be slight and moderate difficulty occurred in the delivery of heifers B and C, respectively. The calf of heifer C, without any abnormal gross-evidences, was stillborn. All animals had retained foetal membranes. A slight increase of the PGF(2alpha) metabolite at the time of parturition was found only in heifer C, whereas the levels dramatically increased in all animals 15-24 h after parturition. At the same time, progesterone levels decreased within 3 h after the first PGF(2alpha) injection (P < 0.05) and reached 0.8, 2.7 and 12.4 nmol/l at the time of parturition in heifers A, B and C, respectively. High release of cortisol at the time of parturition was seen in heifer C. Rising levels of oestrone sulphate around the time of parturition were recorded in all heifers, whereas, increasing levels of PAGs were recorded only in heifer A. In conclusion, the patterns of the PGF(2alpha) metabolite, cortisol, progesterone and PAGs were changed in the cases of calving difficulty and stillbirth after PGF(2alpha)-induction of parturition. However, the relationship between oestrone sulphate and PAGs and the status of foetal well being prior to parturition require further elucidation.
Department of Obstetrics and Gynaecology, Centre for Reproductive Biology in Uppsala, Swedish University of Agricultural Sciences (SLU), SE-750 07 Uppsala, Sweden. bunlue.kornmatitsuk@og.slu.se
B
B
Kornmatitsuk
M C
MC
Veronesi
A
A
Madej
E
E
Dahl
E
E
Ropstad
J F
JF
Beckers
M
M
Forsberg
H
H
Gustafsson
H
H
Kindahl
D000818:Animals; D002417:Cattle / Q000097:blood*; D002418:Cattle Diseases / Q000097:blood; D015237:Dinoprost / Q000008:administration & dosage / Q000097:blood; D004420:Dystocia / Q000097:blood / Q000662:veterinary; D004970:Estrone / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D005313:Fetal Death / Q000097:blood; D005323:Fetal Monitoring / Q000662:veterinary*; D005865:Gestational Age; D006728:Hormones / Q000097:blood*; D006854:Hydrocortisone / Q000097:blood; D007700:Kinetics; D007751:Labor, Induced / Q000662:veterinary; D007743:Labor, Obstetric / Q000097:blood*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis; D011374:Progesterone / Q000097:blood
D006728:Hormones; D011257:Pregnancy Proteins; D004970:Estrone; D011374:Progesterone; D015237:Dinoprost; C017296:estrone sulfate; D006854:Hydrocortisone