Novel Sxr(a) ES cell line offers hope for Y chromosome gene-targeted mice.
A mouse targeted for a Y Chromosome gene has not been reported. Because the Y Chromosome is present in only one copy, and most of its genes are critical for germ cell development, such a mouse would likely be infertile. Thus, we describe a new reproductive strategy to enable transmission of targeted Y Chromosome genes to subsequent generations. The strategy uses two segregating copies of Y Chromosome genes to mimic the autosomal condition. To achieve this, we developed a new embryonic stem cell line from the XYSxr(a) mouse, which carries a duplication of the gene-rich Y Chromosome short arm. Importantly, we demonstrate germ line transmission of the YSxr(a) chromosome and describe this significant new tool as a practical solution to enable reproduction in mice targeted for Y Chromosome genes.
Simpson
Elizabeth M
EM
Centre for Molecular Medicine and Therapeutics, British Columbia Research Institute for Children's and Women's Health, and Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada. simpson@cmmt.ubc.ca
Johnson
Kevin A
KA
Shirley
Bobbi-Jo
BJ
Fang
Ge Y
GY
Bayleran
Janet K
JK
Lerner
Charles P
CP
D000818:Animals; D002460:Cell Line; D020440:Gene Duplication; D018390:Gene Targeting; D007178:Inbreeding; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013234:Stem Cells; D014998:Y Chromosome