Expression of activation markers on alveolar macrophages in allergic asthmatics after endobronchial or whole-lung allergen challenge.
We examined the effect of endobronchial (EB) or whole-lung (WL) challenge with ragweed or Timothy grass extract on alveolar macrophage (AM) activation. Expression of 17 constitutive activation markers on AM was examined by flow cytometry. Late-phase bronchial obstruction was greater after WL challenge, while changes in bronchoalveolar lavage cytology (eosinophil accumulation) were greater after EB challenge. After EB challenge, levels of 10 of 17 markers (CD11a, CD11b, CD14, CD18, CD23, CD32, CD63, CD64, HLA-class I, and HLA-DR) were significantly increased (by 33-234%, P < 0.05). Six markers (CD16, CD29, CD33, CD35, CD44, CD71, and HLA-DQ) remained unchanged. Levels of seven markers following EB challenge (CD14, CD16, CD18, CD29, CD32, HLA-class I, and HLA DQ) correlated with airway sensitivity to methacholine. WL challenge only increased expression of HLA-class I. The different results obtained with the two challenge methods probably depend on higher local concentrations of allergen in the EB challenge. We suggest that activation of AM occurs following EB challenge with antigen in asthmatics.
Department of Medicine, Johns Hopkins Asthma and Allergy Center, Johns Hopkins University School of Medicine, Baltimore, MD 21224-6801, USA.
Michael Y
MY
Viksman
Bruce S
BS
Bochner
R Stokes
RS
Peebles
Robert P
RP
Schleimer
Mark C
MC
Liu
D000328:Adult; D000485:Allergens / Q000276:immunology / Q000494:pharmacology; D015703:Antigens, CD / Q000096:biosynthesis; D001249:Asthma / Q000276:immunology*; D015415:Biomarkers; D001985:Bronchial Provocation Tests; D005260:Female; D006683:HLA-DQ Antigens / Q000096:biosynthesis; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D008168:Lung / Q000276:immunology*; D008262:Macrophage Activation / Q000276:immunology*; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000276:immunology*; D008297:Male
D000485:Allergens; D015703:Antigens, CD; D015415:Biomarkers; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens