Distribution and characterization of microsatellites in the emu (Dromaius novaehollandiae) genome.
This study generates data concerning the genome of a flightless species of bird, the emu. We examined and ultimately rejected the following hypotheses: (1) Microsatellites are randomly distributed throughout the emu genome. (2) The relative order of abundance of dinucleotides will be constant across genomes. (3) Interspersion distances for a given dinucleotide will be equal across vertebrate genomes. (4) In all genomes, a dinucleotide will be more frequent than any trinucleotide. (5) The percentage of single-copy DNA will remain the same in emus as in other volant birds. A cosmid library representing 4.48% of the emu genome was probed with 23 microsatellites. Hybridizations were scored on a scale of 0-3. The average insert size, approximately 40 kb, was used to determine frequency and interspersion. The cosmid library was probed with genomic DNA to determine the percent single copy. Co-occurrence frequencies and confidence intervals were compared to expected using chi-squared. The genome is estimated to contain a microsatellite repeat every 48 kb. Of 1632 clones probed for single-copy DNA, 643 displayed maximal hybridization, 220 displayed moderate hybridization, and 202 had minimal hybridization. After 3 days, 567 showed no hybridization.
Department of Biological Sciences, Texas Tech University, Box 43131, Lubbock, TX 79409, USA. ehroots@aol.com
E H
EH
Roots
R J
RJ
Baker
D000818:Animals; D001717:Birds / Q000235:genetics*; D015342:DNA Probes; D015723:Gene Library; D016678:Genome; D018895:Microsatellite Repeats
D015342:DNA Probes