Establishing two-dimensional gels for the analysis of Leishmania proteomes.
Several different sample preparation methods for two-dimensional electrophoresis (2-DE) analysis of Leishmania parasites were compared. From this work, we were able to identify a solubilization method using Nonidet P-40 as detergent, which was simple to follow, and which produced 2-DE gels of high resolution and reproducibility.
Institute of Biochemistry, University of Lausanne, Switzerland.
Nathalie
N
Acestor
Slavica
S
Masina
John
J
Walker
Nancy G
NG
Saravia
Nicolas
N
Fasel
Manfredo
M
Quadroni
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007891:Leishmania / Q000737:chemistry* / Q000235:genetics; D008970:Molecular Weight; D020543:Proteome / Q000737:chemistry*; D015800:Protozoan Proteins / Q000032:analysis*
D020543:Proteome; D015800:Protozoan Proteins