In vivo effects of bisphenol A on the polecat (mustela putorius).
Bisphenol A (BPA), an environmental estrogen derived from the plastic industry, was given orally via incorporation into the food of 30 male and female polecats at 3 different doses (10, 50, or 250 mg/kg body weight/day) for 2 wk with 10 animals acting as controls. Several hormone levels in the plasma were determined as well as the activities of the phase I and II biotransformation enzymes 7-ethoxyresorufin O-deethylase (EROD), cytosolic glutathione S-transferase (GST), and UDP-glucuronosyltransferase (UDPGT). BPA did not cause any macroscopic effects on body mass or the health of the animals. UDPGT and GST activities increased significantly in direct correlation with increasing BPA exposure in females and UDPGT increased in a dose-related manner in males. There was no change in the plasma T4 and testosterone concentrations of the males with increasing BPA exposure. Discriminant analysis indicated that the group receiving 10 mg BPA/kg body weight/d was not different from the control group but the groups receiving 50 and 250 mg/kg body weight/d were different from the control group. This suggests physiological changes in the groups receiving 50 or 250 mg BPA/kg body weight/d.
Department of Biology, University of Joensuu, Joensuu, Finland. pniemine@cc.joensuu.fi
Petteri
P
Nieminen
Pirjo
P
Lindström-Seppä
Minna
M
Juntunen
Juha
J
Asikainen
Anne-Mari
AM
Mustonen
Sirkka-Liisa
SL
Karonen
Helena
H
Mussalo-Rauhamaa
Jussi V K
JV
Kukkonen
D000284:Administration, Oral; D000818:Animals; D001559:Benzhydryl Compounds; D001835:Body Weight / Q000187:drug effects; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D016002:Discriminant Analysis; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity; D004789:Enzyme Activation / Q000187:drug effects; D004958:Estradiol / Q000097:blood; D004968:Estrogens, Non-Steroidal / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D005289:Ferrets; D005640:Follicle Stimulating Hormone / Q000097:blood; D014453:Glucuronosyltransferase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006854:Hydrocortisone / Q000097:blood; D020738:Leptin / Q000097:blood; D008099:Liver / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D010636:Phenols / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D012737:Sex Factors; D013739:Testosterone / Q000097:blood; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D019363:Cytochrome P-450 CYP1A1; D004785:Environmental Pollutants; D004958:Estradiol; D004968:Estrogens, Non-Steroidal; D005640:Follicle Stimulating Hormone; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase; D006854:Hydrocortisone; D020738:Leptin; D010636:Phenols; D013739:Testosterone; D013972:Thyrotropin; D013974:Thyroxine; D014284:Triiodothyronine; C006780:bisphenol A; D000098826:Bisphenol A Compounds