JH probe real-time quantitative polymerase chain reaction assay for Bcl-2/IgH rearrangements.
Follicular lymphoma (FL) characteristically bears the t(14;18)(q32;q21). However, only approximately 75% of the consequent Bcl-2 breakpoints lie within the major breakpoint region (MBR) or the minor cluster region (mcr). While these can be quantified by cluster region-specific real-time quantitative polymerase chain reaction (RQ-PCR), a significant proportion of cases are left requiring a customized approach. Therefore, an RQ-PCR assay for the quantification of Bcl-2/IgH breakpoints has been developed that uses germline JH TaqMan probes and germline JH primers in combination with customized forward primers. Validation of this approach by comparison with an established MBR RQ-PCR showed both techniques to be concordant across a wide range of copy numbers with a sensitivity of five copies per 10(5) cells. In addition, to generate standard curves equating to diverse Bcl-2/IgH rearrangements, a strategy for using placental DNA as a surrogate standard was devised. The performance of the assay in detecting molecular evidence of disease in sequential biopsies from five patients (three with atypical Bcl-2/IgH breakpoints identified by long-range or inverse PCR, one MBR+ and one mcr+) was tested. This alternative approach represents a sensitive and specific means of quantifying common and atypical Bcl-2/IgH rearrangements and maximizes the number of patients with FL suitable for molecular monitoring.
CRUK Medical Oncology Unit, Department of Histopathology, St. Bartholomew's Hospital, West Smithfield, London EC1A 6BE, UK. jenner@icrf.icnet.uk
Michael J
MJ
Jenner
Karin E
KE
Summers
Andrew J
AJ
Norton
John A
JA
Amess
Rachael S
RS
Arch
Bryan D
BD
Young
T Andrew
TA
Lister
Jude
J
Fitzgibbon
Lindsey K
LK
Goff
D019457:Chromosome Breakage; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics*; D015321:Gene Rearrangement / Q000235:genetics; D019254:Genes, bcl-2; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008224:Lymphoma, Follicular / Q000235:genetics*; D016133:Polymerase Chain Reaction
D007143:Immunoglobulin Heavy Chains