Enzymatic properties of a new peptide deformylase from pathogenic bacterium Leptospira interrogans.
Peptide deformylase (LiPDF), a target protein for antibacterial agents from pathogenic bacteria Leptospira interrogans was identified and purified. Enzymatic studies including kinetics and inhibition revealed new inspiring highlights. The purified active enzyme was a dimer and showed a hyperbolic progress plot when the substrate was low but an excess substrate inhibition effect in higher substrate concentration. Variants on the metal-binding ligand-Cys102 were constructed to verify the indispensable attribute. Also the variant, LiPDF with the insertion residues (R(70)Y(71)P(72)G(73)T(74) P(75)D(76)V(77)) between the conserved motif 1 and motif 2 excised, was constructed and displayed no marked changes on enzymatic features. The results of atom absorbance proved that it contains a tightly bound Zn2+ rather than Fe2+ in E. coliPDF that is an essential cofactor for its high catalytic activity.
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.
Yikun
Y
Li
Zhifeng
Z
Chen
Weimin
W
Gong
D000581:Amidohydrolases; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D003553:Cystine / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D019281:Dimerization; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007919:Leptospira / Q000201:enzymology*; D008024:Ligands; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation; D010802:Phylogeny; D010957:Plasmids / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D013997:Time Factors; D015032:Zinc / Q000737:chemistry
D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D008024:Ligands; D003553:Cystine; D000626:Aminopeptidases; D000581:Amidohydrolases; C084507:peptide deformylase; D015032:Zinc