Various cationic carriers for in vitro transfection of tumor and endothelial cell lines.
We compared the efficiency of in vitro DNA transfer into selected tumor and endothelial cell lines using complexes of plasmid DNA and cationic carriers: DDAB/DOPE, DC-Chol/DOPE, Arg-Chol/DOPE, Gly-Chol/DOPE, Arg-Gly-Chol/DOPE, BGTC/DOPE, and PEI. The best carriers for transfecting the majority of tested cells lines at optimized carrier-to-DNA weight ratios were PEI and BGTC/DOPE.
Department of Molecular Biology, Center of Oncology-Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland.
Barbara
B
Zemlińska
Aleksander
A
Sochanik
Ewa
E
Missol-Kolka
Stanisław
S
Szala
D002460:Cell Line; D004247:DNA / Q000008:administration & dosage*; D017930:Genes, Reporter / Q000502:physiology; D005822:Genetic Vectors; D008081:Liposomes; D014162:Transfection / Q000379:methods*
D008081:Liposomes; D004247:DNA