Molecular cloning, mapping and characterization of a human CK1gamma1 gene.
We isolated and sequenced a cDNA clone coding a human protein kinase CK1 (casein kinase 1) by screening a human fetal brain cDNA library. This new cDNA clone of 1756 bp contained an open reading frame, encoding a protein of 438 amino acids with a molecular weight of 50,272 Da and an isoelectric point of 9.37. The entire amino acid sequence of the novel human CK1 was 94% homologous to that of rat CK1gamma1. Northern blot analysis indicated that the human CK1gamma1 was highly expressed in the liver, skeletal muscle, heart and kidney. Furthermore, the human CK1gamma1 gene was mapped to chromosome 15q22 between STS marker D15S159 and D15S125 by polymerase chain reaction analysis of human/rodent hybrid cell panels.
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China.
Lingchen
L
Guo
Min
M
Jiang
Yushu
Y
Ma
Haipeng
H
Cheng
Xiaohua
X
Ni
Yuhong
Y
Ma
Chaoneng
C
Ji
Yi
Y
Xie
Yumin
Y
Mao
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology / Q000201:enzymology; D047388:Casein Kinases; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005326:Fetal Proteins / Q000235:genetics; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D007526:Isoelectric Point; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D005326:Fetal Proteins; D007527:Isoenzymes; D011494:Protein Kinases; D047388:Casein Kinases