Detection of Microsporidia, cryptosporidia and Giardia in swimming pools: a one-year prospective study.
In order to estimate the rate of microsporidia, cryptosporidia and giardia contamination of swimming pools, sequential samples of water were collected during a one-year period in six different swimming pools in Paris, France. Fourty-eight samples were submitted to filtrations. Eluates were examined for microsporidia using polymerase chain reaction (PCR) and for cryptosporidia and giardia using immunofluorescence staining. One of 48 specimens was positive for microsporidia. Using DNA sequence analysis, unknown microsporidia species were identified, which were close to an insect microsporidia Endoreticulatus schubergi. One sample was positive for cryptosporidia and none were positive for giardia. This study shows a low level of swimming pool water contamination by microsporidia, cryptosporidia or giardia, demonstrating the efficacy of cleaning filtration and disinfection procedures used in French swimming pools.
Division of Infectious Diseases, Saint Louis Hospital, 1 avenue Claude Vellefaux, 75010, Paris, France. sandra.fournier@sls.ap-hop-paris.fr
Sandra
S
Fournier
S
S
Dubrou
O
O
Liguory
F
F
Gaussin
M
M
Santillana-Hayat
C
C
Sarfati
J M
JM
Molina
F
F
Derouin
D000818:Animals; D003458:Cryptosporidium / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000032:analysis; D005374:Filtration; D005455:Fluorescent Antibody Technique; D005602:France; D005872:Giardia / Q000302:isolation & purification*; D016814:Microsporidia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D013551:Swimming Pools; D014867:Water / Q000469:parasitology*
D016054:DNA, Protozoan; D014867:Water