Doxycycline Dependent Expression of Epstein-Barr Virus Latent Membrane Protein 1 with Tet Regulating System in Nasopharygeal Carcinoma Cell Line.
Latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) plays a vital role in cell transformation and the nasopharygeal carcinoma (NPC) is highly related to EBV infection. To elucidate whether LMP1 exerts oncogenic effect in NPC and its possible mechanism, a dual-stable LMP1 integrated NPC cell line with Tet-on regulating system were established. Results revealed that expression of LMP-1 could be regulated by Tet system when NPC cell line HNE(2) served as receipt cell, and doxycycline could increase this expression by about 10 fold. Therefore, it is possible that different level expression of LMP-1 results in its different biological functions and various efforts on nasopharyngeal carcinogenesis.
Cancer research Institute of Hunan Medical University, Changsha 410078, China. ycao98@public.cs.hn.cn
Wei
W
Liao
Hong
H
Yi
Xiao-Yan
XY
Li
Ming
M
Tang
Huan-Hua
HH
Gu
Ya
Y
Cao