Molecular Cloning of Human beta-arrestin1 cDNA, Expression and Functional Study.
Complete coding sequences of beta-arrestin1 (1A and 1B) were cloned through application of bioinformatics analysis to the dbEST database. beta-arrestin1A was overexpressed in E.coli with partial expression products as inclusion body. Anti-beta-arrestin1 antibodies were prepared by using purified inclusion body. Results also demonstrate that activation of inhibitory G protein mediated by delta and kappa pioid receptors was strongly attenuated by overexpression of beta-arrestin1A in co-transfected 293 cells.
Yu
Qing-Ming
QM
Shanghai Institute of Cell Biology, the Chinese Academy of Sciences, Shanghai 200031, China. gangpei@sunm.shcnc.ac.cn
Cheng
Zhi-Jie
ZJ
Zhou
Tian-Hua
TH
Cen
Bo
B
Guo
Sheng-Qi
SQ
Pei
Gang
G