5HT and 5HIAA dialysate levels within the arcuate nucleus of the hypothalamus: relationship with photoperiod-driven differences in serum prolactin and luteinizing hormone in the Siberian hamster.
This study examined the relationship between dialysate levels of serotonin (5HT), and its major metabolite 5HIIAA within the arcuate nucleus of the hypothalamus (ARC) and serum gonadotropin levels under two different in vivo paradigms. Experiment 1 evaluated the relationship between dialysate levels of 5HT and 5HIAA within the ARC and circulating prolactin (PRL) and lutenizing hormone (LH) levels under long- and short-day photoperiod conditions. In experiment 2, the profile of 5HT and 5HIAA dialysate levels within the ARC on the afternoon of proestrous was investigated to determine if changes in serotonergic neurotransmission are correlated with preovulatory surges in LH and PRL. Adult male and female Siberian hamsters were housed either in long-day (16L:8D) or short-day (10L:14D) photoperiods for 8 weeks. Dialysis samples were collected every hour for 5 h (12.00-17.00 h) and blood samples were collected via a jugular cannula every hour for analysis of LH and PRL levels. ARC 5HT and 5HIAA dialysate levels were significantly higher in short-day exposed female hamsters, correlating with suppressed basal LH and PRL secretion when compared to their long-day counterparts. Short-day housed male hamsters displayed significantly higher dialysate levels of 5HIAA than males exposed to a long-day photoperiod-5HT was below the lower limit of detection regardless of photoperiod exposure. Long-day females in proestrus showed no change in dialysate levels of 5HT or 5HIAA within the ARC just prior to the onset of the afternoon surge of LH and PRL. Our results indicate that elevated 5HT and 5HIAA dialysate levels within the ARC may regulate photoperiod effects upon LH and PRL secretion, but not the preovulatory surges of LH and PRL.
Behavioral Neuroscience Division, Psychology Department, SUNY at Buffalo, NY, USA. jdodge@bio.umass.edu
James C
JC
Dodge
Lori L
LL
Badura
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D004563:Electrochemistry; D030762:Estrous Cycle / Q000502:physiology; D005260:Female; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood*; D008297:Male; D017551:Microdialysis; D017111:Phodopus; D017440:Photoperiod; D011359:Proestrus / Q000378:metabolism / Q000502:physiology; D011388:Prolactin / Q000097:blood*; D011863:Radioimmunoassay; D012701:Serotonin / Q000378:metabolism*; D012727:Sex Characteristics
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D011388:Prolactin; D007986:Luteinizing Hormone