Analysis of repeatability in spotted cDNA microarrays.
We report a strategy for analysis of data quality in cDNA microarrays based on the repeatability of repeatedly spotted clones. We describe how repeatability can be used to control data quality by developing adaptive filtering criteria for microarray data containing clones spotted in multiple spots. We have applied the method on five publicly available cDNA microarray data sets and one previously unpublished data set from our own laboratory. The results demonstrate the feasibility of the approach as a foundation for data filtering, and indicate a high degree of variation in data quality, both across the data sets and between arrays within data sets.
Jenssen
Tor-Kristian
TK
Department of Computer and Information Science, Norwegian University of Science and Technology, NO-7491 Trondheim, Norway. tkj@idi.ntnu.no
Langaas
Mette
M
Kuo
Winston P
WP
Smith-Sørensen
Birgitte
B
Myklebost
Ola
O
Hovig
Eivind
E
D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards*; D015203:Reproducibility of Results; D013223:Statistics as Topic