Effect of glucose on glycerol bioconversion by Lactobacillus reuteri.
The impact of glucose on glycerol metabolism, especially on 3-hydroxypropionaldehyde (3-HPA) accumulation by resting cells of Lactobacillus reuteri has been investigated. Two systems were used in the study: MRS(-) (modified MRS - omitting glucose, acetate and Tween 80) and distilled water (H(2)O). In MRS(-), addition of glucose enhanced glycerol metabolism in resting cells of L. reuteri, consequently increasing the accumulation of 3-HPA by regulating the NAD/NADH ratio. Enhanced glycerol metabolism correlated positively with the concentration of glucose. NADH produced during glucose metabolism was preferentially reoxidized to NAD by the reduction of 3-HPA to 1,3-propanediol; an adequate supply of glycerol therefore outweighed the repression of glucose on the accumulation of 3-HPA. At a molar ratio of glucose to glycerol no greater than 0.33, accumulation of 3-HPA was favored. In non-growing medium (H(2)O), addition of glucose seemed to be counter-productive with respect to 3-HPA accumulation. Lactate had a positive impact on glycerol metabolism, presumably by altering the redox flux, resulting in enhanced 3-HPA accumulation in both MRS(-) and H(2)O systems.
Laboratory of Dairy Science, Institute of Food Science and Nutrition, Swiss Federal Institute of Technology Zürich, Switzerland. qiaoqian.luethi-peng@ilw.agrl.ethz.ch
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Dileme
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D000447:Aldehydes; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology*; D005985:Glyceraldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D019344:Lactic Acid / Q000494:pharmacology; D007778:Lactobacillus / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D011407:Propane
D000447:Aldehydes; D003470:Culture Media; D009243:NAD; C047158:3-hydroxypropionaldehyde; D019344:Lactic Acid; D005985:Glyceraldehyde; D005947:Glucose; D005990:Glycerol; D011407:Propane