The gene expression sequence of radiated mucosa in an animal mucositis model.
Oral mucositis is a common, dose-limiting, acute toxicity of radiation therapy administered for the treatment of cancers of the head and neck. Accumulating data would suggest that the pathogenesis of mucositis is complex and involves the sequential interaction of all cell types of the oral mucosa, as well as a number of cytokines and elements of the oral environment. While a number of studies have reported on gene expression of particular cell types in response to radiation, the overall response of irradiated mucosa has only been evaluated in a limited way. The present study was undertaken to evaluate the expression of a target group of genes using RNA quantification assays and, more broadly, to assess patterns of mucosal gene expression using DNA microarray hybridization. Our results demonstrate the sequential upregulation of a series of genes that, when taken collectively, suggest an intricate functional interaction.
Sonis
S T
ST
Division of Oral Medicine, Oral and Maxillofacial Surgery and Dentistry, Brigham and Women's Hospital and the Department of Otal Medicine and Diagnostic Sciences, Harvard School of Dental Medicine, USA. ssonis@partners.org
Scherer
J
J
Phelan
S
S
Lucey
C A
CA
Barron
J E
JE
O'Donnell
K E
KE
Brennan
R J
RJ
Pan
H
H
Busse
P
P
Haley
J D
JD
D000818:Animals; D006224:Cricetinae; D004258:DNA Polymerase III / Q000235:genetics; D004195:Disease Models, Animal; D015870:Gene Expression / Q000528:radiation effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000503:physiopathology*; D019001:Nitric Oxide Synthase / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D020411:Oligonucleotide Array Sequence Analysis; D011833:Radiation Injuries, Experimental / Q000235:genetics* / Q000503:physiopathology; D013280:Stomatitis / Q000235:genetics* / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D014409:Tumor Necrosis Factor-alpha; D016159:Tumor Suppressor Protein p53; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D004258:DNA Polymerase III