Petunia actin-depolymerizing factor is mainly accumulated in vascular tissue and its gene expression is enhanced by the first intron.
Actin-depolymerizing factor (ADF) is one of the actin cytoskeleton-modulating proteins. We have characterized the accumulation pattern of petunia ADF proteins. PhADF proteins are accumulated in every petunia organ and their accumulation is differentially regulated by developmental signals. Their cellular localization is vascular tissue-preferential in vegetative organs, whereas somewhat different in reproductive organs. In reproductive organs, PhADFs are present in outer integument, endocarp of ovary wall, transmitting tissue of style, and epidermis and endothecium of young anther. From a petunia genomic library, we have isolated a genomic clone encoding PhADF1. Comparison to complementary DNA sequence revealed that the coding region of PhADF1 gene consists of three exons and two introns. Analysis of chimeric gene expression using beta-glucuronidase as a reporter gene in transgenic Arabidopsis revealed that PhADF1 was strongly expressed in every vegetative tissue except petal. In addition, expression of the gene was highly enhanced by its first intron. These results suggest that PhADF1 gene of petunia is mainly expressed in vascular tissues and its expression is regulated by intron-mediated enhancement mechanism.
School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea
Jeong-Hwan
JH
Mun
So-Young
SY
Lee
Hee-Ju
HJ
Yu
Young-Min
YM
Jeong
Mi-Young
MY
Shin
Hoyeun
H
Kim
Ilha
I
Lee
Sang-Gu
SG
Kim
D051339:Actin Depolymerizing Factors; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D051345:Destrin; D005091:Exons; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics*; D015151:Immunoblotting; D007150:Immunohistochemistry; D007438:Introns / Q000235:genetics*; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D018514:Plant Structures / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D019657:Solanaceae / Q000235:genetics* / Q000378:metabolism
D051339:Actin Depolymerizing Factors; D018744:DNA, Plant; D051345:Destrin; D018398:Homeodomain Proteins; D008840:Microfilament Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D005966:Glucuronidase