Inactivation of lipid enveloped viruses by octanoic Acid treatment of immunoglobulin solution.
Inactivation of lipid enveloped viruses by treatment with octanoic acid has been investigated for three intravenous immunoglobulin preparations, using Human Immunodeficiency Virus, Bovine Viral Diarrhoea Virus, Sindbis Virus and Pseudorabies Virus as test viruses. At a concentration of 7.45 g octanoic acid per kg solution complete inactivation of lipid enveloped viruses to below detectable level (>5.36, >4.68, >6.25 and >5.55 log(10), respectively) was achieved within the first minutes of treatment. Octanoic acid treatment as described here, has been demonstrated as an effective and rapid virus inactivation procedure, which shows high robustness at the tested ranges of temperature, pH and protein content of the test material. However, pH must be considered as a critical parameter of treatment, as octanoic acid fails to inactivate lipid coated viruses at basic pH. At suitable conditions, e.g. pH<6.0 and a concentration of >3.7 g/kg, octanoic acid treatment gives reliable and highly effective inactivation of lipid enveloped viruses.
Dichtelmüller
H
H
Biotest Pharma GmbH, Dreieich, Germany.
Rudnick
D
D
Kloft
M
M
D000998:Antiviral Agents; D002210:Caprylates / Q000494:pharmacology*; D001908:Diarrhea Viruses, Bovine Viral / Q000187:drug effects; D004340:Drug Contamination / Q000517:prevention & control; D015497:HIV-1 / Q000187:drug effects; D011558:Herpesvirus 1, Suid / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000378:metabolism; D007700:Kinetics; D050356:Lipid Metabolism; D012089:Mammalian orthoreovirus 3 / Q000187:drug effects; D012845:Sindbis Virus / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D038301:Virus Inactivation; D014780:Viruses / Q000187:drug effects / Q000302:isolation & purification*
D000998:Antiviral Agents; D002210:Caprylates; D007074:Immunoglobulin G