[Effect of cilostazol on adhesion molecules of STZ-induced diabetic rats].
OBJECTIVE
To investigate the effect of cilostazol on the expression of CD(54), CD(106), CD(62P) in kidney, aorta, surface of platelet, and mononuclear cell of blood in STZ-induced diabetic rats.


METHODS
Rats were randomly divided into four groups: normal control group (n = 8), diabetes control group (n = 8), insulin group (n = 7 subcutaneous injection of insulin, 1 approximately 4 U * d(-1)), and cilostazol group (n = 7, gastric infusion of cilostazol, 18 mg * kg(-1) * d(-1)). Sciatic nerve conductive velocity and level of CD(54)/CD(62p) on the surface of mononuclear cell/platelet were examined by flow cytometry. Immunohistochemistry was used to examine the expression of CD(54) and/or CD(106) in kidney and aorta.


RESULTS
The sciatic nerve conductive velocity was 20.3 +/- 2.2 m * s(-1) and 28.9 +/- 7.9 m * s(-1) in diabetic rats and cilostazol group respectively (P < 0.05). The mononuclear cell surface expression of CDa-a(c)54 was 69.1 +/- 14.9% and 25.9 +/- 8.6% in diabetic group and cilostazol group respectively (P < 0.05). The expression of CD(54)/CD(106) in kidney and aorta was decreased. The expression of CD(62p) on the surface of platelets was 40.4 +/- 8.7% in diabetic group and 28.5 +/- 3.7% in cilostazol group (P > 0.05).


CONCLUSIONS
Cilostazol treatment delays the development of diabetic pathological change in kidney, aorta and sciatic nerve and decreases the expression of CD(54) and CD(106).
Department of Endocrinology, Shandong Provincial Hospital, Shandong University, Jinan, China.
Zhe
Z
Wang
Jiajun
J
Zhao
Ling
L
Gao
Qunye
Q
Zhang
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000187:drug effects*; D000077407:Cilostazol; D005434:Flow Cytometry; D006442:Glycated Hemoglobin / Q000187:drug effects / Q000378:metabolism; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D019007:P-Selectin / Q000096:biosynthesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000378:metabolism*; D013777:Tetrazoles / Q000494:pharmacology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology*
D015815:Cell Adhesion Molecules; D000077407:Cilostazol; D006442:Glycated Hemoglobin; D018799:Intercellular Adhesion Molecule-1; D019007:P-Selectin; D013311:Streptozocin; D013777:Tetrazoles; D019010:Vascular Cell Adhesion Molecule-1; D014665:Vasodilator Agents