Catalogue of soluble proteins in the human vitreous humor: comparison between diabetic retinopathy and macular hole.
Two-dimensional gel electrophoresis and mass spectrometry were used to make a catalogue of soluble proteins in the human vitreous humor (VH). Fifty-one different proteins were identified on silver-stained two-dimensional (2D) gel patterns with VH proteins obtained from diabetic retinopathy and macular hole. Thirty of these have not been listed in the reported 2D profiles of plasma. Immunoglobulin (Ig), alpha1-antitrypsin, alpha2-HS glycoprotein,and complement C(4) fragment showed stronger spots in VH with diabetic retinopathy patient samples than those with macular hole. Pigment epithelium-derived factor, a potent inhibitor of angiogenesis in the cornea and vitreous, was clearly detected in VH with diabetes. It is impressive that the inhibitor increases in the vitreous with proliferative angiogenesis.
Nakanishi
Toyofumi
T
Department of Clinical Pathology, Osaka Medical College, 2-7 Daigakucho, Takatsuki, Osaka 569-8686, Japan.
Koyama
Reiko
R
Ikeda
Tsunehiko
T
Shimizu
Akira
A
D000595:Amino Acid Sequence; D003930:Diabetic Retinopathy / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005136:Eye Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D012995:Solubility; D014822:Vitreous Body / Q000378:metabolism*
D005136:Eye Proteins