Role of long-range repulsive forces in organizing axonal neurofilament distributions: evidence from mice deficient in myelin-associated glycoprotein.
When the axon of a motor neuron is sectioned and visualized by electron microscopy, a two-dimensional distribution of neurofilaments (NFs) with nonrandom spacing is revealed; this ordered arrangement implies the presence of physical interactions between the NFs. To gain insight into the molecular basis of this organization, we characterized NF distributions from mouse sciatic nerve cross sections using two statistical mechanical measures: radial distribution functions and occupancy probability distributions. Our analysis shows that NF organization may be described in terms of effective pairwise interactions. In addition, we show that these statistical mechanical measures can detect differences in NF architecture between wild-type and myelin-associated glycoprotein null mutant mice. These differences are age dependent, with marked contrast between the NF distributions by 9 months of age. Finally, using Monte Carlo simulations, we compare the experimental results with predictions for models in which adjacent NFs interact through rigid cross bridges, deformable cross bridges, and long-range repulsive forces. Among the models tested, a model in which the filaments interact through a long-range repulsive force is most consistent with the results of our analysis.
Kumar
Sanjay
S
Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Yin
Xinghua
X
Trapp
Bruce D
BD
Paulaitis
Michael E
ME
Hoh
Jan H
JH
D000375:Aging; D000818:Animals; D001369:Axons; D051379:Mice; D018345:Mice, Knockout; D008854:Microscopy, Electron; D008959:Models, Neurological; D009010:Monte Carlo Method; D018992:Myelin-Associated Glycoprotein / Q000172:deficiency* / Q000235:genetics; D009454:Neurofibrils; D012584:Sciatic Nerve / Q000166:cytology
D018992:Myelin-Associated Glycoprotein