Capillary electrophoretic detection in apolipoprotein E genotyping.
We describe the application of capillary electrophoresis to detect DNA fragments, obtained after amplifying a part of the apolipoprotein E (apoE) gene with polymerase chain reaction (PCR). Compared to conventional agarose slab gel electrophoresis (AGE), CE appears the method of choice with regard to resolution and sensitivity, to detect DNA fragments in the range of 20-100 base pairs. Especially discrimination between apoE2/E2 and apoE2/E3 genotypes is more reliable with CE than with AGE, this being of great clinical value in the diagnosis of familiary dysbetalipoproteinemia.
Department of Clinical Chemistry, University Hospital Maastricht, Maastricht, The Netherlands. bekers@klinchem.azm.nl
Otto
O
Bekers
Robert A M
RA
op den Buijsch
Johan E
JE
de Vries
Petal A H M
PA
Wijnen
Marja P
MP
van Dieijen-Visser
D053329:Apolipoprotein E2; D053318:Apolipoprotein E3; D001057:Apolipoproteins E / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013997:Time Factors
D053329:Apolipoprotein E2; D053318:Apolipoprotein E3; D001057:Apolipoproteins E; D004247:DNA