CD11b+ neutrophils predominate over RAM11+ macrophages in stretch-injured muscle.
The purpose of this study was to determine whether both neutrophils and macrophages infiltrate the hematoma site of stretch-injured rabbit tibialis anterior muscle. The Mab.198 antibody was used to detect CD11b(+) neutrophils or macrophages. Neutrophils were identified specifically by using the RPN3/57 antibody. The RAM11 antibody was used to detect macrophages. The histological characteristics of the hematoma site, torn fibers or inflammatory cells, were present primarily at 4 and 24 h, but not at 48 and 72 h after injury. A difference in the Mab.198(+) cellular concentration was detected over time between uninjured and injured muscles (P = 0.03). The injured-uninjured difference in the RPN3/57(+) or RAM11(+) cellular concentrations approached significance (P = 0.07) or else was deemed insignificant (P = 0.13), respectively. Therefore, neutrophils may predominate over RAM11(+) macrophages in stretch-injured muscle. These findings may influence the antiinflammatory strategies used to treat stretch injuries.
School of Nursing, University of Wisconsin, Clinical Science Center, Room K6/364, 600 N. Highland Avenue, Madison, Wisconsin 53792-2455, USA. bsschneider@facstaff.wisc.edu
Barbara
B
St  Pierre Schneider
Stacey
S
Brickson
David T
DT
Corr
Thomas
T
Best
D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D015415:Biomarkers / Q000032:analysis; D002452:Cell Count; D004195:Disease Models, Animal; D018450:Disease Progression; D006406:Hematoma / Q000473:pathology; D007150:Immunohistochemistry; D016177:Macrophage-1 Antigen / Q000096:biosynthesis*; D008264:Macrophages / Q000378:metabolism / Q000473:pathology*; D018482:Muscle, Skeletal / Q000473:pathology* / Q000503:physiopathology; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology*; D011817:Rabbits; D013180:Sprains and Strains / Q000473:pathology* / Q000503:physiopathology; D013314:Stress, Mechanical; D019415:Torque
D000943:Antigens, Differentiation; D015415:Biomarkers; D016177:Macrophage-1 Antigen