[The effects of intermittent hydromechanics on the differentiation and function of bone marrow stromal derived-osteoblasts in porous calcium phosphate ceramics].
OBJECTIVE
To observe the effects of the intermittent hydromechanical stimulation on the differentiation and function of the bone marrow stromal derived-osteoblasts in the porous calcium phosphate ceramics.


METHODS
Rat bone marrow stromal derived-osteoblasts were seeded into porous calcium phosphate ceramic scaffolds at cells density 1 x 10(6) cells/cm(3). The cells-ceramics contrusts were cultured under rotary condition for 1 hour at 4 hours interval. After 4, 7 and 14 days cultivation, the osteoblastic phenotype markers (ALP Activity, Type I Collagen, Osteocalcin, Osteopontin, Osteonectin, and Bone Sialoprotein mRNA expression levels) were analyzed by biochemistry measurement and quantitative RT-PCR technique. Static cell culture as control.


RESULTS
Under rotary cell culture condition, the ALP activities and expressions of Type I collagen mRNA increased markedly and reached the peak levels at 7 days. The expressions of other four markers mRNA occurred at 4 days and reached the peak levels at 7 days, then the expressions were down-regulated at 14 days. Under static cell culture as control, the ALP activities and expressions of Type I collagen mRNA increased gradually and reached the peak levels at 14 days. The expressions of other four markers mRNA occurred at 7 days and reached the peak levels at 14 days.


CONCLUSIONS
The intermittent hydromechanical stimulation could promote the bone marrow stromal derived-osteoblasts differentiation and function which cultured in the porous calcium phosphate ceramics in vitro.
Department of Orthopedics, Third Hospital of Peking University, Beijing 100083, China.
Kai
K
Tang
Gengting
G
Dang
Zhaoqing
Z
Guo
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001696:Biomechanical Phenomena; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002130:Calcium Phosphates / Q000494:pharmacology*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D002516:Ceramics; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D058574:Integrin-Binding Sialoprotein; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D053495:Osteopontin; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012399:Rotation; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism
D002130:Calcium Phosphates; D024042:Collagen Type I; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D015676:Osteonectin; D012333:RNA, Messenger; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D015675:Osteocalcin; D053495:Osteopontin; D000469:Alkaline Phosphatase