In vitro antioxidant potentials of traditional Chinese medicine, Shengmai San and their relation to in vivo protective effect on cerebral oxidative damage in rats.
The preventive effects of Shengmai San (SMS), a traditional Chinese herbal medicine (TCM), was studied on cerebral ischemia-reperfusion injury in rats as a model of antioxidant-based composite therapy. Two biochemical indicators of oxidative damage, thiobarbituric acid reactive substance (TBARS) formation and glutathione peroxidase (GPX) loss were measured in the brain after forebrain ischemia-reperfusion treatment and both were inhibited in all rats administered SMS (15 g original herbs/kg) 2 h before the ischemia-reperfusion. Histochemical study of the brain slice using TTC staining revealed that the SMS effectively reduced infarct area caused by the cerebral ischemia-reperfusion. The antioxidant potentials of SMS preparations were determined in vitro by five different assay methods and were related to the in vivo effectiveness of SMS in protection against brain damage. Inhibitory effect on TBARS formation in vivo showed better correlation with superoxide radical scavenging and DPPH quenching activity in vitro rather than with the other in vitro antioxidant indicators. On the other hand, the in vivo prevention of GPX activity loss showed better correlation with in vitro crocin bleaching inhibition than with the other in vitro antioxidant indicators. It was also suggested that the in vitro TBARS inhibitory activty of SMS is not a good indication to predict the in vivo effectiveness of SMS on inhibition of either TBARS formation or GPX activity loss.
Department of Radiochemistry-Biophysics, Niigata University of Pharmacy and Applied Life Science, Japan.
Haruyo
H
Ichikawa
Tetsuya
T
Konishi
D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D016166:Free Radical Scavengers / Q000494:pharmacology / Q000627:therapeutic use*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008516:Medicine, Chinese Traditional; D018384:Oxidative Stress / Q000187:drug effects*; D016548:Prosencephalon / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D017392:Thiobarbituric Acid Reactive Substances; C009252:fructus schizandrae, radix ginseng, radix ophiopogonis drug combination; D005979:Glutathione Peroxidase