Predominance of the DEN-3 genotype during the recent dengue outbreak in Bangladesh.
A recent outbreak of dengue in Bangladesh was marked by many fatal complications. As clinical virulence varies among the genotypes of dengue virus, a study was conducted to investigate the molecular genotypes of dengue in Bangladesh. Reverse transcription polymerase chain reaction was used to determine viral genotypes using oligonucleotide generic primers that produce a 511 bp product. The resulting product was typed by nested PCR with strain-specific primers, yielding 482 (DEN-1), 119 (DEN-2), 290 (DEN-3) and 392 (DEN-4), visualized on UV transilluminator after electrophoresis on 2% agarose gel stained with ethidium bromide. Of 45 clinically diagnosed dengue patients (mean age 28 years; male/female 30/15), 19 (42.2%) had detectable viral RNA in their blood. However, during the first 5 days of fever in 30 patients, the frequency was 60% (18/30), implying that the sooner serum is drawn after the fever, the greater the chances of detecting viral RNA. DEN-3 was detected in all except 2 patients who were infected with DEN-2. DEN-2 (two cases) and DEN-4 (one case) were present as co-infections with DEN-3. All of the patients presented with fever, anorexia and vomiting; many had headache and general body ache; a few had a rash. About a quarter had suffered episodes of bleeding, while ascites, pleural effusion and CNS symptoms were found in a few patients Patients positive for viral RNA were also positive for anti-dengue IgM (p=0.007) in subsequent sampling. The study suggests the predominance of DEN-3 infection with occasional co-infection with other types, during the recent outbreak of dengue in Bangladesh.
Aziz
M M
MM
Department of Immunology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders BIRDEM, Dhaka.
Hasan
K N
KN
Hasanat
M A
MA
Siddiqui
M A
MA
Salimullah
M
M
Chowdhury
A K
AK
Ahmed
Moslehuddin
M
Alam
M N
MN
Hassan
M S
MS
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D001459:Bangladesh / Q000453:epidemiology; D001483:Base Sequence; D017931:DNA Primers; D003715:Dengue / Q000453:epidemiology* / Q000821:virology; D003716:Dengue Virus / Q000235:genetics* / Q000276:immunology; D004196:Disease Outbreaks; D006801:Humans; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000235:genetics*
D000914:Antibodies, Viral; D017931:DNA Primers; C084188:E protein, Dengue virus type 3; D012367:RNA, Viral; D014759:Viral Envelope Proteins