Group II Phospholipase A(2) Activity and Its Molecular Regulation in Rat Heart during Sepsis.
Changes of activity and content of myocardial group II phospholipase A(2) and its mRNA transcription and stability during rat sepsis were investigated. Results showed that, compared with control group,myocardial group II phospholipase A(2) activity in early and late sepsis decreased by 25.0%(P<0.05) and increased by 47.6%(P<0.01),respectively group II phospholipase A(2) protein concentration reduced by 27.0% and augmented by 48.0%(P<0.01),respectively. Myocardial group II phospholipase A(2) mRNA transcription rate and content showed similar two-phases changes. The mRNA transcription rate during early and late sepsis decreased by 45.0% and increased by 70.0%(P<0.01),respectively. The mRNA content decreased by 34.1% in early sepsis and increased by 157.0% in late sepsis(P<0.01), respectively. The half-life of group II phospholipase A(2) mRNA remained unchanged notably during early and late stage of sepsis. These data suggest that myocardial group II phospholipase A(2) activity decreased in early stage of sepsis and increased in its late stage, and these changes were regulated transcriptionally.
Jiang
Zhi-Sheng
ZS
Institute of Cardiovascular Basic Research, Beijing Medical University, Beijing 100083, China. zsjiang@public.bta.net.cn
Dong
Lin-Wang
LW
Yang
Jun
J
Tong
Li-Jia
LJ
Pang
Yong-Zheng
YZ
Tang
Chao-Shu
CS
Liu
Nai-Kui
NK