Molecular species of glycinin in some soybean cultivars.
A(4) polypeptide-containing (Shirotsurunoko and York) and A(4) polypeptide-lacking (Raiden and Suzuyutaka) soybean cultivars were used to investigate the heterogeneity of glycinin molecular species. Purification of glycinin by DEAE-Toyopearl column chromatography afforded molecular species eluting before the glycinin fraction. Analysis of this fraction by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and sucrose density gradient centrifugation indicated that this protein consisted of A(1) and A(2) polypeptides. The A(4)-containing soybean cultivars contained less of this protein than the A(4)-lacking soybean cultivars, as exhibited by the size of the early peak appearing during column chromatography. Alkaline PAGE and N-terminal amino acid sequence analysis confirmed that the A(1)- and A(2)-rich molecular species in the A(4) polypeptide-lacking cultivars consisted of the A(1a) and A(2) polypeptides. Estimation of the molecular mass by gel permeation chromatography and multi-angle laser light scattering (GPC-MALLS) indicated that the A(1a)- and A(2)-rich molecular species were similar to a monomer of glycinin.
Graduate School of Agriculture, Kyoto University, Uji, 611-0011, Kyoto, Japan.
Guo Yan
GY
Zhang
Yukako
Y
Hayashi
Shinya
S
Matsumoto
Yasuki
Y
Matsumura
Tomohiko
T
Mori
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005916:Globulins / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D030262:Soybean Proteins; D013025:Glycine max / Q000737:chemistry*
D005916:Globulins; D030262:Soybean Proteins; C011474:glycinin