Crystallization and preliminary X-ray analysis of the TRAF domain of TRAF3.
Tumor necrosis factor receptors (TNFR) signal events in immune responses, Ig class switching, activation of NF-kappaB or regulation of apoptosis. TNFR-associated factors (TRAFs) are adaptor proteins that connect TNFRs to downstream signaling pathways, including the NF-kappaB and c-JUN N-terminal kinase (JNK) pathways. Members of the TRAF family exist as trimers and share a conserved TRAF domain that mediates binding to the cytoplasmic domains of TNFRs. The TRAF domain from TRAF3 has been crystallized. In addition, an N-terminally truncated form of the domain has been crystallized in space group P321 with a shortened c axis and markedly improved diffraction (2.5 A resolution).
Ni
Chao Zhou
CZ
Cancer Research Center, The Burnham Institute, La Jolla, CA 92037, USA.
Welsh
Kate
K
Zheng
Jianhua
J
Havert
Marnie
M
Reed
John C
JC
Ely
Kathryn R
KR
D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D015394:Molecular Structure; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D048008:TNF Receptor-Associated Factor 3
D011506:Proteins; D011994:Recombinant Proteins; D048008:TNF Receptor-Associated Factor 3