Directional guidance of oligodendroglial migration by class 3 semaphorins and netrin-1.
Oligodendrocytes, the myelin-forming cells of the CNS, are generated from multiple foci distributed along the developing neural tube. Little is known about the endogenous guidance cues controlling the migration of oligodendrocyte precursor cells (OPCs) from their site of emergence toward their final destination, mainly the future white matter tracts. During embryonic development, the optic nerve is populated by OPCs originating in the diencephalon that migrate from the chiasm toward the retina. Here we show that OPCs migrating into the embryonic optic nerve express the semaphorin receptors neuropilin-1 and -2, as well as deleted in colorectal cancer (DCC) and, to a lesser extend unc5H1, two of the netrin-1 receptors. Using a functional migration assay, we provide evidence that Sema 3A and netrin-1 exert opposite chemotactic effects, repulsive or attractive, respectively, on embryonic OPCs. In addition, we show that Sema 3F has a dual effect, chemoattractive and mitogenic on embryonic OPCs. The localization of cells expressing Sema 3A, Sema 3F, and netrin-1 is consistent with a role for these ligands in the migration of OPCs in the embryonic optic nerve. Altogether, our results suggest that the migration of OPCs in the embryonic optic nerve is modulated by a balance of effects mediated by members of the semaphorin and netrin families.
Spassky
Nathalie
N
Biologie des Interactions Neurones, Glie Institut National de la Santé et de la Recherche Médicale U-495, Hôpital de la Salpêtrière, 75651 Paris, France.
de Castro
Fernando
F
Le Bras
Barbara
B
Heydon
Katharina
K
Quéraud-LeSaux
Françoise
F
Bloch-Gallego
Evelyne
E
Chédotal
Alain
A
Zalc
Bernard
B
Thomas
Jean-Léon
JL
D000818:Animals; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D019070:Cell Lineage; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology; D046508:Culture Techniques; D000075389:DCC Receptor; D006023:Glycoproteins / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D009414:Nerve Growth Factors / Q000378:metabolism* / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000378:metabolism* / Q000494:pharmacology; D000075386:Netrin Receptors; D000075388:Netrin-1; D039942:Neuropilin-1; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009900:Optic Nerve / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D040121:Semaphorin-3A; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis
D015815:Cell Adhesion Molecules; D000075389:DCC Receptor; C501909:DCC protein, human; C501910:Dcc protein, mouse; D006023:Glycoproteins; D008565:Membrane Proteins; C000617351:NTN1 protein, human; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D000075386:Netrin Receptors; C000617353:Ntn1 protein, mouse; D011956:Receptors, Cell Surface; C491230:SEMA3F protein, human; C491229:Sema3f protein, mouse; D040121:Semaphorin-3A; D025521:Tumor Suppressor Proteins; C000617308:Unc5A protein, mouse; D039942:Neuropilin-1; D000075388:Netrin-1