Control of autogenous activation of Herbaspirillum seropedicae nifA promoter by the IHF protein.
Analysis of the expression of the Herbaspirillum seropedicae nifA promoter in Escherichia coli and Herbaspirillum seropedicae, showed that nifA expression is primarily dependent on NtrC but also required NifA for maximal expression under nitrogen-fixing conditions. Deletion of the IHF (integration host factor)-binding site produced a promoter with two-fold higher activity than the native promoter in the H. seropedicae wild-type strain but not in a nifA strain, indicating that IHF controls NifA auto-activation. IHF is apparently required to prevent overexpression of the NifA protein via auto-activation under nitrogen-fixing conditions in H. seropedicae.
Departamento de Bioquímica - UFPR, Caixa Postal 19046, CEP 81531-990, Curitiba, PR Brazil.
Roseli
R
Wassem
Fábio O
FO
Pedrosa
Marshall G
MG
Yates
Fabiane G M
FG
Rego
Leda S
LS
Chubatsu
Liu U
LU
Rigo
Emanuel M
EM
Souza
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D020562:Betaproteobacteria / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology*; D005798:Genes, Bacterial; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000502:physiology; D009586:Nitrogen Fixation / Q000235:genetics / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology; D051922:PII Nitrogen Regulatory Proteins; D011401:Promoter Regions, Genetic / Q000235:genetics; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D034982:Integration Host Factors; C061338:NifA protein, Bacteria; D051922:PII Nitrogen Regulatory Proteins; D000644:Quaternary Ammonium Compounds; D015534:Trans-Activators; D014157:Transcription Factors; C497876:glnG protein, E coli; D010100:Oxygen