Karyotype analysis of a Korean cucumber cultivar (Cucumis sativus L. cv. Winter Long) using C-banding and bicolor fluorescence in situ hybridization.
An intensive karyotype analysis of a Korean cucumber cultivar (Cucumis sativus L. cv. Winter Long) was carried out with three different methods. These included Feulgen staining, Giemsa C-banding, and fluorescence in situ hybridization (FISH). The mitotic chromosomes of the cucumber (2n = 2x = 14) were characterized, based on the length and arm ratio values. A C-banding analysis showed dark stains on the centromeric, telomeric, and intercalary regions of the chromosomes, except that chromosome 2 had a heavy staining in the long arm. Bicolor FISH, using 45S and 5S rDNA probes, provided additional information to identify cucumber chromosomes. The signals for 45S rDNA were detected on the pericentromeric regions of chromosomes 1, 2, and 4. The signals for 5S rDNA were on the short arm of chromosome 5. Similar band patterns (as the C-banding) were observed when the chromosomes were counter-stained with 4',6-diamidino-2-phenyoindole (DAPI). The data implied that the karyotype of the Korean cucumber cultivar is peculiar and different from previous reports.
Koo
Dal-Hoe
DH
Department of Biology, Chungnam National University, Daejeon, Korea.
Hur
Yoonkang
Y
Jin
Dong-Chun
DC
Bang
Jae-Wook
JW
D002871:Chromosome Banding; D004396:Coloring Agents; D018553:Cucumis sativus / Q000235:genetics*; D020732:Cytogenetic Analysis / Q000379:methods*; D004275:DNA, Ribosomal / Q000235:genetics; D017343:Genes, Plant; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007723:Korea; D020161:Physical Chromosome Mapping; D012394:Rosaniline Dyes; D013194:Staining and Labeling
D004396:Coloring Agents; D004275:DNA, Ribosomal; C007722:Feulgen stain; D012394:Rosaniline Dyes