Two-dimensional protein analysis by isoelectric focusing in a multi-cell plate system.
A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.
Laboratorio de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego 2000, CEP 28015-620, Campos dos Goytacazes-RJ, Brazil.
Beatriz
B
dos Santos Ferreira
Alan Trindade
AT
Branco
Karlla Salim
KS
de Queiroz
Gonçalo Apolinàrio
GA
de Souza Filho
D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004926:Escherichia coli; D007525:Isoelectric Focusing; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D003313:Zea mays
D001426:Bacterial Proteins; D010936:Plant Extracts