Prevalence of the CCR5Delta32 mutation in Brazilian populations and cell susceptibility to HIV-1 infection.
We investigated the occurrence of the CCR5Delta32 mutation in various regional ethnic groups in Brazil and tested the resistance of mutant peripheral blood mononuclear cells (PBMCs) to infection by HIV-1 in vitro. The heterozygous prevalence was 5.3% in uninfected African descendents and 8.8% in HIV-1-positive individuals (neither population had Delta32/Delta32). German descendents were 11% heterozygous and l% Delta32/Delta32. Amerindians were exclusively CCR5/CCR5. Heterozygous uninfected PBMCs showed partial resistance to R5-HIV-1 strains in vitro, but no resistance to X4 virus. HIV-1-positive CCR5/CCR5 had higher viral loads than did heterozygous cells.
Escola Bahiana de Medicina e Saúde Pública/Fundação para o Desenvolvimento das Ciências, Salvador-Bahia, Brasil.
Rogério
R
Grimaldi
Nice
N
Shindo
Angelina Xavier
AX
Acosta
Inês
I
Dourado
Carlos
C
Brites
Ozenilda
O
de Melo Carvalho
Ivo
I
Brito
Dumith Chequer
DC
Bou-Habib
Bernardo
B
Galvão-Castro
D000483:Alleles; D001938:Brazil / Q000453:epidemiology; D002478:Cells, Cultured; D018450:Disease Progression; D018562:Disease Transmission, Infectious; D017353:Gene Deletion; D020022:Genetic Predisposition to Disease; D015658:HIV Infections / Q000453:epidemiology* / Q000235:genetics*; D015497:HIV-1 / Q000254:growth & development*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D009154:Mutation; D015995:Prevalence; D019713:Receptors, CCR5 / Q000235:genetics*
D019713:Receptors, CCR5