Plaque assay for African swine fever virus on swine macrophages.
A plaque assay developed to detect the infection of African Swine Fever Virus on swine macrophages is described. Plaques were generated by all of the virus isolates tested. The method is suitable not only for virus titration but also for the selection of clones in protocols for isolation/purification of recombinant viruses.
Centro de Biología Molecular Severo Ochoa (C.S.I.C.-U.A.M.), Universidad Autónoma de Madrid, Spain.
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Bustos
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Nogal
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Carrascosa
D000358:African Swine Fever Virus / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D002478:Cells, Cultured; D016676:Macrophages, Alveolar / Q000821:virology*; D013552:Swine; D010948:Viral Plaque Assay / Q000379:methods*