T(-786)-->C polymorphism of the endothelial nitric oxide synthase gene influences the progression of renal disease.
OBJECTIVE
Polymorphism of the endothelial nitric oxide synthase (ecNOS) gene may be involved in renal disease. Recently, T(-786)-->C polymorphism affecting ecNOS gene transcription has been reported. To clarify the role of T(-786)-->C polymorphism in renal disease, we investigated hemodialysis patients and healthy controls for this polymorphism and we compared its frequency with that of intron 4 polymorphism in the hemodialysis patients.


METHODS
The subjects were 252 patients who had been on hemodialysis for less than 2 years (168 with nondiabetic nephropathy and 84 with diabetic nephropathy) and 187 healthy controls. T(-786)-->C polymorphism was detected using polymerase chain reaction-restriction fragment length polymorphism analysis.


RESULTS
The frequencies of the T/C and C/C genotypes were significantly higher in the nondiabetic hemodialysis patients than in the controls (odds ratio 1.41; 95% Cl 1.03-2.00), and were also significantly higher in the diabetic hemodialysis patients than in the controls (odds ratio 1.56; 95% Cl 1.02-2.41). In addition, T(-786)-->C polymorphism and intron 4 polymorphism showed strong linkage disequilibrium.


CONCLUSIONS
T(-786)-->C polymorphism may be involved in the progression of both nondiabetic and diabetic nephropathy, along with intron 4 polymorphism.
Second Department of Internal Medicine, Hiroshima University School of Medicine, Hiroshima City, Japan.
Yukiteru
Y
Asakimori
Noriaki
N
Yorioka
Yoshihiko
Y
Taniguchi
Takafumi
T
Ito
Satoshi
S
Ogata
Yasufumi
Y
Kyuden
Nobuoki
N
Kohno
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000201:enzymology* / Q000235:genetics / Q000473:pathology / Q000628:therapy; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D011110:Polymorphism, Genetic; D006435:Renal Dialysis
C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III