Coordination of mesangial cell contraction by gap junction--mediated intercellular Ca(2+) wave.
Gap junction intercellular communication (GJIC) plays a fundamental role in mediating intercellular signals and coordinating multicellular behavior in various tissues and organs. Glomerular mesangial cells (MC) are rich in GJ, but the functional associations of these intercellular channels are still unclear. This study examines the potential role of GJ in the transmission of intercellular Ca(2+) signals and in the coordination of MC contraction. First, the presence of GJ protein Cx43 and functional GJIC was confirmed in MC by using immunochemical staining or transfer of Lucifer yellow (LY) after a single cell injection, respectively. Second, mechanical stimulation of a single MC initiated propagation of an intercellular Ca(2+) wave, which was preventable by the GJ inhibitor heptanol but was not altered by pretreatment of MC with ATP or addition of apyrase into the assay system. Third, the phospholipase C (PLC) inhibitor U73122 could largely eliminate the mechanically elicited propagation of intercellular Ca(2+) waves, suggesting a possibly mediating role of inositol trisphosphate (IP(3)) in the initiation and transmission of intercellular Ca(2+) signaling. Fourth, injection of IP(3) into a single cell caused contraction, not only in the targeted cell, but also in the adjacent cells, as indicated by the reduction of cellular planar area. Fifth, addition of two structurally unrelated GJ inhibitors, heptanol and alpha-glycyrrhetinic acid (GA), into MC embedded in collagen gels significantly attenuated the reduction of gel areas after exposure to serum. This study provides the first functional evidence supporting the critical role of GJIC in the synchronization of MC behaviors.
Yao
Jian
J
Department of Cellular Physiology, Institute of Nephrology, Niigata University, Niigata, Japan.
Morioka
Tetsuo
T
Li
Bing
B
Oite
Takashi
T
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology*; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006034:Glycyrrhetinic Acid / Q000494:pharmacology; D019850:Heptanol / Q000494:pharmacology; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism*; D051381:Rats; D010738:Type C Phospholipases / Q000378:metabolism
D015544:Inositol 1,4,5-Trisphosphate; D019850:Heptanol; D010738:Type C Phospholipases; D006034:Glycyrrhetinic Acid; D002118:Calcium