The identification and properties of chitin-binding protein b01 (CBPb01).
Bovine serum contains N-acetyl-D-glucosamine (GlcNAc)-sensitive opsonin inhibitory factors. In the present study, a major component of chitin-binding protein (chitin-binding protein b01, CBPb01) was purified from bovine serum, and identified CBPb01 as bovine IgM by its subunit structure, antigenic properties, and partial sequences. The results of a lectin-binding assay showed that the heavy chain of CBPb01 had a GlcNAc structure, but the commercial IgM did not. It is possible that CBPb01 interconnects through its GlcNAc structure, subsequently forming complexes. We also demonstrated that CBPb01 had opsonin-inhibitory activity, and that this activity was dependent on the binding of CBPb01 to GlcNAc on the zymosan surface. These findings indicate the presence of a kind of IgM that recognizes GlcNAc structure in the regulation of opsonization.
Suzuki
Masako
M
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Japan.
Morimatsu
Masami
M
Syuto
Bunei
B
D000117:Acetylglucosamine / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002417:Cattle; D002686:Chitin / Q000378:metabolism*; D002850:Chromatography, Gel / Q000662:veterinary; D005260:Female; D005779:Immunodiffusion / Q000662:veterinary; D007075:Immunoglobulin M / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009895:Opsonin Proteins / Q000378:metabolism*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D015054:Zymosan / Q000378:metabolism
D001798:Blood Proteins; D007075:Immunoglobulin M; D009895:Opsonin Proteins; D002686:Chitin; D015054:Zymosan; D000117:Acetylglucosamine