Butane and propane oxidation by engineered cytochrome P450cam.
The haem monooxygenase cytochrome P450cam has been engineered to oxidise the gaseous alkanes butane and propane to butan-2-ol and propan-2-ol, respectively, by the use of bulky amino acid substitutions to reduce the volume of the substrate pocket and thus improve the enzyme-substrate fit: the F87W/Y96F/T101L/V247L mutant oxidizes butane with a turnover rate of 750 min-1 and 95% yield based on NADH consumed while the wild-type enzyme has an activity of 0.4 min-1 with 4% yield.
Bell
Stephen G
SG
Department of Chemistry, Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, UK OX1 3QR.
Stevenson
Julie-Anne
JA
Boyd
Helen D
HD
Campbell
Sophie
S
Riddle
Austin D
AD
Orton
Erica L
EL
Wong
Luet-Lok
LL
D001665:Binding Sites / Q000235:genetics; D002073:Butanes / Q000378:metabolism*; D000440:Butanols; D019475:Camphor 5-Monooxygenase / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D010084:Oxidation-Reduction; D011407:Propane / Q000378:metabolism*; D020005:Propanols; D015202:Protein Engineering; D016958:Pseudomonas putida / Q000201:enzymology
D002073:Butanes; D000440:Butanols; D020005:Propanols; C046888:butane; D019475:Camphor 5-Monooxygenase; D011407:Propane