Reaction diffusion model of the enzymatic erosion of insoluble fibrillar matrices.
Predicting the time course of in vivo biodegradation is a key issue in the design of an increasing number of biomedical applications such as sutures, tissue analogs and drug-delivery devices. The design of such biodegradable devices is hampered by the absence of quantitative models for the enzymatic erosion of solid protein matrices. In this work, we derive and simulate a reaction diffusion model for the enzymatic erosion of fibrillar gels that successfully reproduces the main qualitative features of this process. A key aspect of the proposed model is the incorporation of steric hindrance into the standard Michaelis-Menten scheme for enzyme kinetics. In the limit of instantaneous diffusion, the model equations are analogous to the standard equations for enzymatic degradation in solution. Invoking this analogy, the total quasi-steady-state approximation is used to derive approximate analytical solutions that are valid for a wide range of in vitro conditions. Using these analytical approximations, an experimental-theoretical method is derived to unambiguously estimate all the kinetic model parameters. Moreover, the analytical approximations correctly describe the characteristic hyperbolic dependence of the erosion rate on enzyme concentration and the zero-order erosion of thin fibers. For definiteness, the analysis of published experimental results of enzymatic degradation of fibrillar collagen is demonstrated, and the role of diffusion in these experiments is elucidated.
School of Computer Science and Engineering, Department of Neurobiology, The Hebrew University, Jerusalem 91904, Israel. ramitz@cs.huji.ac.il
Abraham R
AR
Tzafriri
Michel
M
Bercovier
Hanna
H
Parnas
D001665:Binding Sites; D001673:Biodegradation, Environmental; D055592:Biophysical Phenomena; D001703:Biophysics; D004058:Diffusion; D004798:Enzymes / Q000737:chemistry*; D024022:Fibrillar Collagens / Q000737:chemistry*; D005782:Gels / Q000737:chemistry; D007700:Kinetics; D008962:Models, Theoretical; D013997:Time Factors
D004798:Enzymes; D024022:Fibrillar Collagens; D005782:Gels