A Novel Myotoxin from the Venom of Trimeresurus mucrosquamatus.
A novel myotoxin, designated TMPB, was purified from the venom of Trimeresurus mucrosquamatus by Sephadex G-100 superfine gel chromatography and fast protein liquid chromatography (FPLC). The N-terminal sequence of 24 amino acid residues was determined by protein sequencer. The sequence similarities between TMPB and other two phospholipase A(2) (PLA2s) previously purified from the same venom were 41.7% and 54.2%, respectively, but TMPB showed no detectable PLA(2) hydrolytic activity. Its molecular weight was estimated to be 16 000 by reducing SDS-PAGE and isoelectric point was determined to be 9.2 by isoelectric focusing electrophoresis. TMPB exhibited strong myotoxicity and platelet aggregation inhibiting activity, and the two activities could all be inhibited by heparin.
Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China. xutr@163.net
Tian-Rui
TR
Xu
Wan-Yu
WY
Wang
Qing-Xiong
QX
Meng
Yu-Hui
YH
Huang
Qiu-Ming
QM
Lu
Yu-Liang
YL
Xiong