Diboronic acids as fluorescent probes for cells expressing sialyl Lewis X.
A series of fluorescent diboronic acids was synthesized in nine steps as potential sensors for sialyl Lewis X (sLex). The fluorescent binding studies of these sensors with sLex were carried out in a mixed aqueous solution. Compound 7e was found to show the strongest fluorescence enhancement upon binding with sLex. Using cell cultures, 7e was shown to label sLex-expressing HEPG2 cells at 1 microM, while non-sLex-expressing cells were not labeled.
Yang
Wenqian
W
Department of Chemistry, North Carolina State University, Raleigh, NC 27695-8204, USA.
Gao
Shouhai
S
Gao
Xingming
X
Karnati
Vishnu Vardhan Reddy
VV
Ni
Weijuan
W
Wang
Binghe
B
Hooks
W Borden
WB
Carson
John
J
Weston
Brent
B
D000818:Animals; D001665:Binding Sites; D001897:Boronic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D019556:COS Cells; D005453:Fluorescence; D015870:Gene Expression; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D000080506:Sialyl Lewis X Antigen; D013194:Staining and Labeling / Q000379:methods*; D014407:Tumor Cells, Cultured
D001897:Boronic Acids; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen