Immobilized earthworm fibrinolytic enzyme III-1 with carbonyldiimidazole activated-agarose.
Earthworm fibrinolytic enzyme III-1 (EFE-III-1) was prepared to couple with cross-linking agarose activated by 1,'-Carbonyl- diimidazole (CDI) in this study. Although the activity of the immobilized protease decreased to approximately 64% of the native enzyme, the activity of EFE-III-1 coupled with the resin activated by CDI was higher than that activated by cyanogen bromide (CNBr). The immobilized protease was experimentally demonstrated to hydrolyze IgG, albumin and creatine kinase, besides fibrin(ogen) and plasmin(ogen), suggesting that EFE had a broad substrate specificity.
Wu
Xue-Qin
XQ
Lab of Visual Information Processing, Academic Center of Brain and cognition, Institute of Biophysics, The Chinese Academy of Sciences, 15 Da Tun Rd, Beijing 100101, China.
Wu
Cen
C
He
Rong-qiao
RQ
D000818:Animals; D002417:Cattle; D003402:Creatine Kinase / Q000378:metabolism; D003488:Cyanogen Bromide / Q000494:pharmacology; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D005337:Fibrin / Q000187:drug effects / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000737:chemistry* / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000378:metabolism; D009835:Oligochaeta / Q000201:enzymology*; D012685:Sepharose / Q000737:chemistry; D012709:Serum Albumin / Q000378:metabolism; D013379:Substrate Specificity; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
D004800:Enzymes, Immobilized; D005343:Fibrinolytic Agents; D007093:Imidazoles; D007074:Immunoglobulin G; D012709:Serum Albumin; D013439:Sulfhydryl Reagents; C006900:N,N-carbonyldiimidazole; D005337:Fibrin; D012685:Sepharose; D003402:Creatine Kinase; D003488:Cyanogen Bromide