Quantitative analysis of memantine in human plasma by gas chromatography/negative ion chemical ionization/mass spectrometry.
A sensitive and specific method for the determination of memantine in human plasma is presented. Memantine was extracted from plasma and derivatized to the pentafluorobenzoyl derivative in a one-step procedure avoiding any sample concentration steps. Amantadine was used as an internal standard. The compounds were measured by gas chromatography/negative ion chemical ionization mass spectrometry without any further processing. Using this detection mode, the fragment ions at m/z 353 and 325 were obtained at high relative abundance. Calibration graphs were linear over the range 0.117-30 ng ml(-1). At the limit of quantification (LOQ), the inter-assay precision was 2.00% and the intra-assay variability was 3.22%. The accuracy at the LOQ showed deviations of -1.42% (intra-assay) and -2.47% (inter-assay). The method is rugged, rapid and robust and was applied to the batch determination of memantine during pharmacokinetic profiling of the drug.
University Children's Hospital, Division of Analytical Biochemistry and Mass Spectrometry, Auenbruggerplatz 30, A-8036 Graz, Austria. hans.leis@kfunigraz.ac.at
Hans Jörg
HJ
Leis
Günter
G
Fauler
Werner
W
Windischhofer
D000547:Amantadine / Q000097:blood; D000978:Antiparkinson Agents / Q000097:blood* / Q000493:pharmacokinetics; D001682:Biological Availability; D001793:Blood Preservation; D002138:Calibration; D015925:Cryopreservation; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008559:Memantine / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*
D000978:Antiparkinson Agents; D000547:Amantadine; D008559:Memantine