Immunohistochemical and ultrastructural identification of Fusobacterium necrophorum subsp. necrophorum in bovine fatal necrotizing glossitis.
A 37-day-old male Japanese black calf showing marked salivation and leucocytosis died and was examined the tissues histologically. Histological lesions were characterized by severe focal necrotic glossitis on the ventral side of the root of the tongue. Immunohistochemically, Fusobacterium necrophorum subsp. necrophorum antigen was detected in the necrotic tissues and its distribution corresponded to that of the gram-negative, nonsporeforming, long filamentous organisms. Ultrastructural similarities between the organism and F. necrophorum subsp. necrophorum, but not subsp. funduliforme were observed. These findings clearly demonstrated that the fatal necrotic glossitis was caused by F. necrophorum subsp. necrophorum. This is the first report of bovine fatal necrotizing glossitis with leucocytosis caused by F. necrophorum subsp. necrophorum infection, and this organism may be an important fatal pathogen in calves with glossal lesions.
Hokkaido Research Station, National Institute of Animal Health, Toyohira, Sapporo, Japan.
Tomoyuki
T
Shibahara
Toshifumi
T
Akiba
Taiji
T
Maeda
Takaaki
T
Ogata
Ryusuke
R
Honda
Yoshiharu
Y
Ishikawa
Koichi
K
Kadota
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D017809:Fatal Outcome; D005674:Fusobacterium Infections / Q000378:metabolism / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D005675:Fusobacterium necrophorum / Q000648:ultrastructure*; D005928:Glossitis / Q000378:metabolism / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron / Q000662:veterinary