Immunohistochemical localization and biochemical characterization of ghrelin in the brain and stomach of the frog Rana esculenta.
Ghrelin is a 28-amino acid n-octanoylated peptide recently isolated from the rat stomach as an endogenous ligand of the growth hormone secretagogue receptor. So far, the occurrence of ghrelin has not been investigated in submammalian vertebrates. In the present work, we have studied the anatomic distribution and biochemical characterization of ghrelin-like immunoreactivity in the brain and stomach of the frog Rana esculenta by using two distinct antisera directed against rat ghrelin. In the brain, sparse ghrelin-positive cells were detected in three nuclei of the diencephalon, namely the suprachiasmatic nucleus and the posterior tuberculum in the hypothalamus, and the posterodorsal aspect of the lateral nucleus in the thalamus. A few ghrelin-immunoreactive neurons were also found in the mesencephalon, i.e., in the pretoral gray and the anterodorsal tegmental nucleus. Ghrelin-containing fibers were widely distributed in the frog brain. In particular, diffuse networks of immunoreactive processes were observed in various regions of the telencephalon, including the medial pallium, the striatum, the nucleus of the diagonal band of Broca, the nucleus accumbens, and the amygdala. In the diencephalon, the magnocellular nucleus, the suprachiasmatic nucleus, the posterior tuberculum, and the ventrolateral and lateral thalamic nuclei were moderately to densely innervated with ghrelin-containing fibers. A moderate density of positive fibers was also found in different areas of the mesencephalon such as the nucleus of the medial longitudinal fasciculus, the pretoral gray, and the tegmentum. In the stomach, a few brightly immunofluorescent cells were detected in the mucosa. The distribution pattern and morphologic characteristics of ghrelin-containing cells in the stomach suggest that they correspond to endocrine cells. Reversed-phase high performance liquid chromatography analysis of frog brain and stomach extracts, combined with RIA detection, revealed that ghrelin-immunoreactive material eluted as a single peak with a retention time slightly shorter than that of synthetic rat ghrelin. The present data provide the first evidence that a ghrelin-related peptide is present in submammalian vertebrates. The occurrence of ghrelin-containing cells in the hypothalamus and the stomach mucosa suggests that, in amphibians, ghrelin may exert both neuroendocrine and endocrine activities.
European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U-413, UA CNRS, University of Rouen, 76821 Mont-Saint-Aignan, France.
Ludovic
L
Galas
Nicolas
N
Chartrel
Masayasu
M
Kojima
Kenji
K
Kangawa
Hubert
H
Vaudry
D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004435:Eating / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D019858:Enteroendocrine Cells / Q000166:cytology / Q000378:metabolism*; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism*; D054439:Ghrelin; D013006:Growth Hormone / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D036361:Peptide Hormones; D010455:Peptides / Q000378:metabolism*; D011863:Radioimmunoassay; D011893:Rana esculenta / Q000033:anatomy & histology / Q000378:metabolism*
D054439:Ghrelin; D036361:Peptide Hormones; D010455:Peptides; D013006:Growth Hormone