Purification, crystallization and preliminary X-ray analysis of the soluble domain of the Na+-pumping cytochrome bo quinol oxidase from Vitreoscilla.
The 24 kDa CyoA soluble domain of Vitreoscilla cytochrome bo quinol oxidase, which pumps out Na(+) during respiration, has been crystallized from a solution of 2 M ammonium sulfate and 5% 2-propanol. The crystal belongs to cubic space group P4(3)32, with unit-cell parameters a = b = c = 122.2 A, alpha = beta = gamma = 90 degrees and one subunit in the asymmetric unit. A 99.8% complete data set to 3.3 A has been collected at the 17-ID beamline of the Advanced Photon Source. The structure was determined by molecular replacement and refinement is in progress.
Industrial Macromolecular Crystallography Association Collaborative Access Team (IMCA-CAT), Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA.
Kyung Jin
KJ
Kim
Youngchang
Y
Kim
Kyung Won
KW
Park
Dale A
DA
Webster
Andrew J
AJ
Howard
D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004269:DNA, Bacterial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012995:Solubility; D020589:Vitreoscilla / Q000201:enzymology* / Q000235:genetics
D004269:DNA, Bacterial; C038862:cytochrome o oxidase; D003576:Electron Transport Complex IV; D000254:Sodium-Potassium-Exchanging ATPase