Analysis of protein ions in the range 3000-12000 Th under partial (no discharge) atmospheric pressure chemical ionization conditions using ion trap mass spectrometry.
A new approach, based on the use of atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS), but without a corona discharge, was investigated for application to creating and monitoring protein ions. It must be emphasized that APCI is not usually used in protein analysis. In order to verify the applicability of the proposed method to the analysis of proteins, two standard proteins (horse cytochrome c and horse myoglobin) were analyzed. A mixture of the two proteins was also analyzed showing that this novel approach, based on the use of APCI, can be used in the analysis of protein mixtures.
Cristoni
Simone
S
Università degli Studi di Milano, Centro Interdisciplinare Studi Bio-Molecolari Applicazioni Industriali CISI, Via Fratelli Cervi 93, 20090 Segrate, Milano, Italy. dtoycr@tin.it
Bernardi
Luigi Rossi
LR
Biunno
Ida
I
Guidugli
Federico
F
D000818:Animals; D003574:Cytochrome c Group / Q000032:analysis*; D005021:Ethylamines; D006736:Horses; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D009211:Myoglobin / Q000032:analysis*; D012997:Solvents; D013696:Temperature
D003574:Cytochrome c Group; D005021:Ethylamines; D009211:Myoglobin; D012997:Solvents; C016162:triethylamine