[Comparative evaluation of mono- and neutrophilokins inducing activity of Yersinia pestis antigens].
The results of the comparative analysis of the cytokine inducing activity of Yersinia pestis EV antigens are presented. Y. pestis fraction 1A (F1A) and lipopolysaccharide (LPS) were shown to induce mono- and neutrophilokines, regulating cooperative interaction of phagocytes in the process of immunity formation to plague. Neutrophilokines and monokines exceed in their capacity for inducing F1A such acknowledged inductor as Escherichia coli LPS. As revealed by the comparative evaluation of Y. pestis EV LPS and E. coli LPS, neutrophilokines synthesized under the action of the former preparation, have greater influence on the inhibition of the macrophage migration from the infection focus as well as on digestive activity of these cells (in secondary immune response) and on the labilization of the lysosome membranes of macrophages than neutrophilokines induced by E. coli LPS. At the same time they produce a lesser modulating effect on the killer and chemotactic activity of neutrophils, as well as on the expression of FC receptors (FcR) on their surface in comparison with monokines, synthesized under the influence of E. coli LPS.
Research Institute for Plague Control, Rostov-on-Don, Russia.
G I
GI
Vasil'eva
I A
IA
Ivanova
I A
IA
Bespalova
A K
AK
Kiseleva
E P
EP
Doroshenko
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D002452:Cell Count; D002465:Cell Movement; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008247:Lysosomes / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D010587:Phagocytosis; D011961:Receptors, Fc / Q000032:analysis; D015010:Yersinia pestis / Q000276:immunology*
D000942:Antigens, Bacterial; D016207:Cytokines; D008070:Lipopolysaccharides; D011961:Receptors, Fc