Comparison of cholinesterase activities in the excretion-secretion products of Trichinella pseudospiralis and Trichinella spiralis muscle larvae.
The presence of cholinesterases (ChE) is reported in T. pseudospiralis excretion-secretion products (ESP) by spectrophotometric method, using acetylthiocholine (ATCI) and butyrilthiocholine (BTCI) as substrates. By inhibition assays, we found that T. pseudospiralis release both acetyl- and butiryl-cholinesterases (AchE and BchE, respectively). The sedimentation coefficientes of these enzymes were determined by sucrose density gradient. We studied the in vivo ChE secretion by immunoblot assays using AchE from Electrophorus (electric eel) and sera from normal or infected mice with T. pseudospiralis or T. spiralis. The presence of anti-AchE antibodies was only demonstrated in the sera from T. pseudospiralis infected mice. Moreover the in vivo secretion was corroborated by the high difference determinate between the ChE activity of the immuno complexes from T. pseudospiralis infected sera and the immunocomplexes from T. spiralis infected sera as well as normal sera. Finally, we analyzed the effect of the organophosphate Neguvón (metrifonate) on the ChE activity from the T. pseudospiralis ESP. The drug inhibits in part this activity. Moreover Neguvón (metrifonate) showed a high activity against the T. pseudospiralis viability.
Laboratory of Parasitology, Dpt. Microbiology and Parasitology, Faculty of Pharmacy, University of Alcalá, Crtra. Madrid-Barcelona km 33, 28871 Alcalá de Henares, Madrid, Spain.
R M
RM
Ros-Moreno
C
C
De Armas-Serra
C
C
Gimenez-Pardo
F
F
Rodriguez-Caabeiro
D000110:Acetylcholinesterase / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology; D000909:Antibodies, Helminth / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000032:analysis; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide / Q000494:pharmacology; D002091:Butyrylcholinesterase / Q000032:analysis* / Q000276:immunology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D005260:Female; D015801:Helminth Proteins / Q000032:analysis* / Q000276:immunology; D006790:Host-Parasite Interactions; D007814:Larva / Q000201:enzymology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018482:Muscle, Skeletal / Q000469:parasitology*; D013045:Species Specificity; D013770:Tetraisopropylpyrophosphamide / Q000494:pharmacology; D014234:Trichinella / Q000201:enzymology* / Q000254:growth & development / Q000276:immunology; D017160:Trichinella spiralis / Q000201:enzymology* / Q000254:growth & development / Q000276:immunology; D014236:Trichlorfon / Q000494:pharmacology
D000871:Anthelmintics; D000909:Antibodies, Helminth; D000936:Antigen-Antibody Complex; D002800:Cholinesterase Inhibitors; D015801:Helminth Proteins; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide; D013770:Tetraisopropylpyrophosphamide; D014236:Trichlorfon; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase