Cloning of Glial Cell Line-derived Neurotrophic Factor Gene and Its Expression in Eukaryotic Cells.
Glial cell line-derived neurotrophic factor gene (gdnf) was obtained by RT-PCR method. Its pro-duct in COS7 cells showed neurotrophic and protective effect on the dopaminergic neurons. Then gdnf gene was cloned into retrovirus vector pLNCX and packaged with PA317. Infectious particles thus obtained were used to infect rat myoblast cell line L-6TG. A cell clone L-6TG/gdnf was obtained after G418 selection. It will be applied to the ex vivo gene therapy study of Parkinson's disease.
Jiang
Qiong
Q
Shanghai Institute of Biochemistry the Chinese Academy of Science Shanghai 200031 China. sib203@sunm.shcnc.ac.cn
Liu
Jian
J
Ge
Kai
K
Liu
Wei-Cheng
WC
Sun
Lan-Ying
LY
Liu
Xin-Yuan
XY
Zheng
Zhong-Cheng
ZC