Immunization of cattle with synthetic peptides derived from the Boophilus microplus gut protein (Bm86).
Three synthetic peptides (SBm4912, SBm7462 and SBm19733), derived from the Bm86 glycoprotein from Boophilus microplus gut, were constructed and used to immunize cattle from a tick-free area. The immunized animals received three subcutaneous doses of the peptides, with saponin as adjuvant, at 30-day intervals. The immune response was evaluated by IgG elicited against the peptides by the detection of anti-Bm86 specific antibodies in situ and by Western blotting analysis. After tick challenge, reduction in the number, weight and oviposition capacity of engorged females was observed in the tick population that had fed on immunized animals. The results pointed a high efficacy (81.05%) for the SBm7462 synthetic peptide in relation to the others (p<0.01), demonstrating the efficiency of the immune response elicited by synthetic peptides to control the cattle tick B. microplus.
Laboratory of Biology and Control of Haematozoa, BIOAGRO/Veterinary Department, Federal University of Viçosa, 36571-000, Viçosa, MG Brazil. jpatarro@mail.ufv.br
J H
JH
Patarroyo
R W
RW
Portela
R O
RO
De Castro
J Couto
JC
Pimentel
F
F
Guzman
M E
ME
Patarroyo
M I
MI
Vargas
A A
AA
Prates
M A Dias
MA
Mendes
D000818:Animals; D015153:Blotting, Western; D001835:Body Weight; D002417:Cattle / Q000276:immunology*; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology; D026863:Ixodidae / Q000276:immunology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology*; D010058:Oviposition; D010446:Peptide Fragments / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology*; D011994:Recombinant Proteins; D013984:Tick Infestations / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control* / Q000662:veterinary; D014611:Vaccination / Q000379:methods / Q000662:veterinary; D014612:Vaccines; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*
C085065:BM86 protein, Boophilus; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D014612:Vaccines; D014614:Vaccines, Synthetic