Purification and characterization of a new lectin from the red marine alga Hypnea musciformis.
A lectin from the red marine alga Hypnea musciformis (HML) was purified by extraction with 20 mM PBS, precipitation with 70% saturated ammonium sulphate, ion-exchange DEAE-Cellulose chromatography and RP-HPLC. The 9.3 kDa polypeptide agglutinates erythrocytes from various sources and shows oligomerization tendencies under certain MALDI-TOF/MS conditions. Preliminary N-terminal sequencing and biological assays strongly suggest that the HML may belong to a new class of algae lectins.
Laboratório de Bioquímica Marinha - BioMol-Lab, Depto de Engenharia de Pesca, Universidade Federal do Ceará, Caixa Postal 6033, CEP 60451-970, Brasil.
C S
CS
Nagano
F B M B
FB
Moreno
C
C
Bloch
M V
MV
Prates
J J
JJ
Calvete
S
S
Saker-Sampaio
W R L
WR
Farias
T D
TD
Tavares
K S
KS
Nascimento
T B
TB
Grangeiro
B S
BS
Cavada
A H
AH
Sampaio
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D019281:Dimerization; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification*; D017434:Protein Structure, Tertiary; D000461:Rhodophyta / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D037102:Lectins