Bone and bone marrow interactions: hematological activity of osteoblastic growth peptide (OGP)-derived carboxy-terminal pentapeptide. II. Action on human hematopoietic stem cells.
Osteogenic growth peptide (OGP) is a peptide exerting regulatory effects on the bone and on bone marrow. The carboxy-terminal pentapeptide (OGP10-14) is the biologically active portion of OGP. We evaluated OGP10-14 hematopoietic activity performing colony-forming tests on human stem cells derived by bone marrow, peripheral blood and cord blood. Granulocyte-macrophage colony-forming unit (CFU) were significantly increased in OGP10-14-treated samples, while granulocyte-erythrocyte-monocyte-megakaryocyte CFU and burst-forming unit (BFU) erythroid were increased only in the cord blood test.Moreover, OGP10-14 preserves stem cells self renewal potential in long-term culture (LTC) initiating cells and acts directly on CD34+ enriched cells or by increasing activity of stem cell factor (SCF) and granulocyte-megakaryocyte colony-stimulating factor.
Department of Oncology, Transplants and Advanced Technologies in Medicine, Hematology Division, University of Pisa, Via Roma 67, Pisa, Italy.
Rita
R
Fazzi
Sara
S
Galimberti
Rossana
R
Testi
Simone
S
Pacini
Silvia
S
Trasciatti
Sergio
S
Rosini
Mario
M
Petrini
D018952:Antigens, CD34 / Q000032:analysis; D001773:Blood Cells / Q000166:cytology / Q000187:drug effects; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D001842:Bone and Bones / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D054407:Chemokine CCL4; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D004723:Endorphins / Q000494:pharmacology*; D005312:Fetal Blood / Q000166:cytology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007938:Leukemia / Q000473:pathology; D019402:Macrophage Inflammatory Proteins / Q000032:analysis; D022423:Myeloid Cells / Q000166:cytology; D009928:Organ Specificity; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis; D016185:Receptors, Interleukin-3 / Q000032:analysis; D019089:Stem Cell Factor / Q000494:pharmacology; D017154:Stromal Cells / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000166:cytology
D018952:Antigens, CD34; D054407:Chemokine CCL4; D004723:Endorphins; D016298:Hematopoietic Cell Growth Factors; D019402:Macrophage Inflammatory Proteins; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D016185:Receptors, Interleukin-3; D019089:Stem Cell Factor; C050198:historphin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D019009:Proto-Oncogene Proteins c-kit