Allergenicity of hen's egg ovomucoid gamma irradiated and heated under different pH conditions.
This study was conducted to evaluate the effect of a treatment combining gamma radiation and heating on the allergenic properties of hen's egg ovomucoid (OM) under basic pH conditions. OM solutions of 2.0 mg/ml with pHs of 7.0, 9.0, and 10.0 were gamma irradiated at 10 kGy, heated at 100 degrees C for 15 min, or both. Half of the treated pH 10.0 sample solution was restored to pH 7.4 by dialysis. OM solutions were tested by a competitive direct enzyme-linked immunosorbent assay formatted with immunoglobulin E from egg-hypersensitive patients. An equation was obtained for quantifying intact OM from the standard curve, and the detected concentration of intact OM was calculated. The concentration of intact OM decreased with irradiation or heating, and the rate of the decrease was higher for a basic pH condition than for the physiological condition. The combination of irradiation and heating was very effective in reducing the amount of intact OM regardless of the pH condition. After treatment, the restoration of the pH to 7.4 did not affect the concentration of OM. The results of this study indicate that a combination of irradiation and heating might be an effective method for reducing egg hypersensitivity resulting from OM.
Lee
Ju-Woon
JW
The Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute, Yuseong, Daejeon.
Lee
Kyoung-Youl
KY
Yook
Hong-Sun
HS
Lee
Soo-Young
SY
Kim
Hee-Yun
HY
Jo
Cheorun
C
Byun
Myung-Woo
MW
D000818:Animals; D002645:Chickens; D004531:Eggs; D004797:Enzyme-Linked Immunosorbent Assay; D005512:Food Hypersensitivity / Q000517:prevention & control*; D005720:Gamma Rays; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007073:Immunoglobulin E / Q000276:immunology*; D010059:Ovomucin / Q000276:immunology* / Q000528:radiation effects*; D010940:Plant Proteins / Q000276:immunology / Q000528:radiation effects; D014361:Trypsin Inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D010940:Plant Proteins; D014361:Trypsin Inhibitors; D010059:Ovomucin; D007073:Immunoglobulin E; D000516:alpha-Amylases