Identification of functional p53-binding motifs in the mouse wig-1 promoter.
We previously identified wig-1 as a p53-induced mouse gene that encodes a nuclear zinc finger protein with unknown function. To investigate whether wig-1 is a direct target of p53-dependent transactivation, a DNA fragment corresponding to the promoter region was cloned and sequenced. Three regions containing consensus p53-binding sites were identified. Two p53-binding motifs formed DNA-protein complexes with p53 and were able to drive p53-dependent transcription in a luciferase reporter assay. Our results demonstrate that wig-1 is a direct target of p53-mediated transcriptional transactivation.
Department of Oncology-Pathology, Cancer Center Karolinska (CCK), Karolinska Institute, SE-171 76 Stockholm, Sweden.
Margareta T
MT
Wilhelm
Cristina
C
Méndez-Vidal
Klas G
KG
Wiman
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D051379:Mice; D009687:Nuclear Proteins; D010957:Plasmids; D011401:Promoter Regions, Genetic; D016601:RNA-Binding Proteins; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D016159:Tumor Suppressor Protein p53; C491518:Wig1 protein, mouse; D004247:DNA