Identification of the novel allele B*4427 and a confirmatory sequence (B*44022).
This report presents a novel allele, HLA-B*4427, which was identified in a bone marrow donor of Caucasian origin, and a confirmatory sequence (B*44022). Sequence analysis revealed the new allele differs from B*44021 by a single nucleotide exchange at position 668 (C-->T), which is located in exon 4. At the protein level, it is the only B*44 variant to produce an Ala in place of a Val at codon 199. Its structure suggests that it may have originated from a point mutation in B*44021 or by gene conversion with a variety of HLA-B alleles. Cloning and sequencing of the allele B*44022 revealed a sequence identical to B*44021 and B*44 exon 4, with the codon GTC (Val) in position 199.
Canossi
A
A
CNR Institute of Tissue Typing and Dialysis Regional Center of Immunohematology and Tissue Typing, Department of Experimental Medicine, University of L'Aquila, Italy.
Papola
F
F
Liberatore
G
G
Del Beato
T
T
Piancatelli
D
D
Tessitore
A
A
Vicentini
M T
MT
Maccarone
D
D
Aureli
A
A
Cervelli
C
C
Di Rocco
M
M
Casciani
C U
CU
Adorno
D
D
D000483:Alleles; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D015235:HLA-B Antigens / Q000235:genetics*; D059948:HLA-B44 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
D015235:HLA-B Antigens; C471439:HLA-B*44:27 antigen; D059948:HLA-B44 Antigen