Expression of the Cydia pomonella granulovirus iap3 gene.
The IAP3 protein of Cydia pomonella granulovirus (CpGV) was the first identified member of the baculovirus IAP family of proteins, which have been shown to block apoptosis in diverse systems. However, little is known of the expression and subcellular localisation of CpGV IAP3 during a viral infection. This study examined IAP3 in cells infected by CpGV and in cells infected by an Autographa californica nucleopolyhedrovirus (AcMNPV) recombinant that carried the CpGV iap3 gene. The levels of iap3 specific transcripts were monitored and production of the protein was assessed using an IAP3-specific antiserum. The data showed that iap3 is expressed during both early and late phases of infection, with a switch occurring from distal early transcription start sites to proximal late start sites. Protein levels are highest after DNA replication. IAP3 is localised exclusively in the cytoplasm. Subcellular fractionation experiments demonstrated that the protein is present in both soluble and membrane-bound cytosolic fractions. The membrane-bound fraction includes IAP3 that is associated with the mitochondria. However, the data do not support the hypothesis that release of cytochrome C from the mitochondria is involved in baculovirus-induced apoptosis.
Department of Biological Sciences, Imperial College of Science, Technology and Medicine, Imperial College Road, London, UK.
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DP
Miller
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Luque
N E
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Crook
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Winstanley
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O'Reilly
D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D002478:Cells, Cultured; D025722:Granulovirus / Q000378:metabolism*; D015151:Immunoblotting; D007313:Insecta / Q000821:virology; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera / Q000821:virology; D013347:Subcellular Fractions / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*
D014764:Viral Proteins