Supramolecular properties of the proline-rich gamma-Zein N-terminal domain.
Zeins are maize storages proteins that accumulate inside large vesicles called protein bodies. gamma-Zein lines the inner face of the protein body membrane, and its N-terminal proline-rich repetitive domain with the sequence (VHLPPP)(8) appears to be necessary for the accumulation of the protein within the organelle. Synthetic (VHLPPP)(8) adopts an amphipathic polyproline II conformation. In a preliminary recent work we used atomic force microscopy to study the surface organization of the octamer and transmission electron microscopy to visualize aggregates of the peptide from aqueous solution. We previously envisioned two self-assembly models (i.e., the geometric and the micellar) that take into account the observed features. In the present work we studied in detail the self-assembly of the peptide in solution and found that the peptide is able to form cylindrical micelles. Fibrils formed on graphite are generated by assembly of solution micelles. Based on the results of these studies, we focused exclusively on the micellar model. To our knowledge we have characterized for the first time supramolecular aggregates of polyproline structures other than collagen. The spontaneous arrangement of (VHLPPP)(8) suggests a role for the N-terminal domain of gamma-zein in the process of the whole protein deposition in protein bodies.
Departament de Química Orgànica, Universitat de Barcelona, 08028-Barcelona, Spain.
Marcelo J
MJ
Kogan
Ionara
I
Dalcol
Pau
P
Gorostiza
Carmen
C
Lopez-Iglesias
Ramon
R
Pons
Miquel
M
Pons
Fausto
F
Sanz
Ernest
E
Giralt
D055592:Biophysical Phenomena; D001703:Biophysics; D002942:Circular Dichroism; D005614:Freeze Fracturing; D008823:Micelles; D018625:Microscopy, Atomic Force; D008854:Microscopy, Electron; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry; D011392:Proline / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012542:Scattering, Radiation; D013500:Surface Tension; D014965:X-Rays; D003313:Zea mays / Q000737:chemistry*
D008823:Micelles; D010455:Peptides; D011392:Proline