Glucocorticoids Modulate G-protein alpha-subunit Levels in Rat Hypothalamus.
Glucocorticoids (Gc) affect neuroendocrine function of hypothalamic-pituitary-adrenal axis (HPAA), especially of the hypothalamus. Synthesis and secretion of neuronal hormones of hypothalamus are associated with G-protein-linked signalling system. Here, the regulation by Gc of the levels of several G-protein alpha-subunits in rat hypothalamus was studied with Western blot method. Results showed that Gc downregulated Galphas1, Galphas2,Galphai and Galphaq with different magnitudes, being 14.37,15.66,14.61 and 12.37 per cent, respectively. However, Gc did not affect Galphao expression. Our results provided important information for understanding how Gc alters neuroendocrine function through G-protein-linked signalling system.
Department of Biochemistry, Hebei Medical University, Shijiazhuang 050017, China. huaning@163.net
Bo
B
Gao
Ren-Ping
RP
Xiong
Gui-Shan
GS
Yin
Yuan-Guo
YG
Zhou