Influence of soluble polymer polyvinylpyrrolidone on separation of small peptides and amino acids by microchip-based capillary electrophoresis.
In microchip-based capillary electrophoresis, the resolution and separation efficiency of small peptides and amino acids can be noticeably improved by adding a low molecular weight (30,000) soluble polymer additive, polyvinypyrrolidone in the separation medium. Several separation conditions such as injection time and electrophoretic buffer have been investigated and optimized. Using an electro-stacking scheme, the resolution and separation efficiency of small peptides and amino acids can be enhanced significantly. Under the optimal conditions, the separation of fluorescein isothiocyanate Isomer I-labeled small peptides and amino acids was successfully achieved within 100 s.
Chen
Hong
H
Department of Chemistry, Wuhan University, PR China.
Zeng
Yong
Y
Liu
Bi-Feng
BF
Pang
Dai-Wen
DW
Cheng
Jie-Ke
JK
D000596:Amino Acids / Q000302:isolation & purification*; D002021:Buffers; D004151:Dipeptides / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000592:standards*; D009994:Osmolar Concentration; D011205:Povidone / Q000494:pharmacology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility
D000596:Amino Acids; D002021:Buffers; D004151:Dipeptides; D011205:Povidone