Molecular modelling of chain end effects in separating oligomers by reversed-phase gradient polymer elution chromatography; adsorption transition as revealed by a self-consistent-field theory for polymer adsorption.
HPLC experiments to separate butyl-terminated polystyrene (B-PS) oligomers have been mimicked by equilibrium self-consistent-field calculations based upon the Scheutjens Fleer formalism for polymers at interfaces. The adsorption-desorption transition as a function of the fraction of good solvent in a non-solvent (water)-solvent (tetrahydrofuran) mixture has been analysed and correlated to corresponding experiments. Much attention is paid to keeping the modelling as realistic as possible; for example, the effects of the solvent mixture on the C18-alkyl tails that are grafted on the silica surface are retained in the calculations. It is shown that the butyl end groups affect the elution properties up to chains with approximately 30 styrene units. Excellent semi-quantitative comparison is found with experiments for a realistic set of interaction parameters. Molecular-level information is available for the adsorption layer as a function of the solvent quality. Going from poor to good solvent, it is typical to find that the B-PS is fully absorbed inside the alkyl brush, then adsorbed on top of it, and finally depleted from it. The depletion effect in good solvents increases with increasing molecular mass.
Laboratory of Physical Chemistry and Colloid Science, Wageningen University, The Netherlands. frans@fenk.wau.nl
F A M
FA
Leermakers
H J A
HJ
Philipsen
B
B
Klumperman
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008958:Models, Molecular; D011108:Polymers / Q000737:chemistry*
D011108:Polymers