Scale-up of recombinant protein purification by hydrophobic interaction chromatography.
The scale-up of hydrophobic interaction chromatography is described. Human recombinant superoxide dismutase was used as a model. The scale-up was performed by keeping the height to diameter (H/D) ratio of the column constant. The success of scale-up was evaluated by reversed-phase high-performance liquid chromatography of the eluted material. The wrong H/D ratio causes decreased resolution.
Institut für angewandte Mikrobiologie, Universität für Bodenkultur, Vienna, Austria.
K
K
Vorauer
M
M
Skias
A
A
Trkola
P
P
Schulz
A
A
Jungbauer
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011994:Recombinant Proteins / Q000302:isolation & purification*
D011994:Recombinant Proteins