SVK14 cells express an MCH binding site different from the MCH1 or MCH2 receptor.
Melanin-concentrating hormone (MCH) is a cyclic peptide, mainly involved in the regulation of skin pigmentation in teleosts and feeding behavior in mammals. The human keratinocyte SVK14 cell line has been previously shown to express binding sites for the MCH analog [125I]-[Phe13,3-iodo-Tyr19]MCH. We report here that: (1) this binding site similarly recognized [125I]-[3-iodo-Tyr13]MCH; (2) its pharmacological profile clearly differed from those observed at the two human MCH receptor subtypes, MCH1-R and MCH2-R; (3) MCH did not induce any effect on second messenger systems (including cAMP, calcium, and MAP kinase signaling pathways), and (4) no mRNAs corresponding to the MCH receptors were found. In conclusion, the binding site characterized in the SVK14 cell line is distinct from the MCH1 and MCH2 receptors and deserves therefore further investigation.
Division de Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 125, Chemin de Ronde, Croissy-sur-Seine, France.
Valérie
V
Audinot
Chantal
C
Lahaye
Thomas
T
Suply
Carole
C
Rovère-Jovène
Marianne
M
Rodriguez
Jean Paul
JP
Nicolas
Philippe
P
Beauverger
Bruno
B
Cardinaud
Jean Pierre
JP
Galizzi
Jean Luc
JL
Fauchère
Jean Louis
JL
Nahon
Jean A
JA
Boutin
D001665:Binding Sites; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007028:Hypothalamic Hormones / Q000737:chemistry* / Q000378:metabolism*; D020128:Inhibitory Concentration 50; D015603:Keratinocytes / Q000378:metabolism; D008024:Ligands; D020935:MAP Kinase Signaling System; D008543:Melanins / Q000737:chemistry* / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D010907:Pituitary Hormones / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D011979:Receptors, Pituitary Hormone / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013329:Structure-Activity Relationship
D007028:Hypothalamic Hormones; D008024:Ligands; C431280:MCHR2 protein, human; D008543:Melanins; D010455:Peptides; D010907:Pituitary Hormones; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D011979:Receptors, Pituitary Hormone; C092274:melanin-concentrating hormone receptor; C019463:melanin-concentrating hormone; D000242:Cyclic AMP