Effect of medium composition, flow rate, and signaling compounds on the formation of soluble extracellular materials by biofilms of Chromobacterium violaceum.
Biofilms of the homoserine-lactone-defective strain Chromobacterium violaceum CV026 were grown on silicone surfaces in a flow chamber. The effect of medium flow rate, different levels of N-butanoyl-homoserine lactone, and nutrients on biofilm activity was studied by quantifying the proteins and exopolysaccharides attached to the cells. To compare the effect of each of the three variables within a wide range, the experiments were designed as a full factorial search with three levels for each variable. Calculated contour plots demonstrated that N-acyl homoserine lactone is an important determinant of both the amount and the composition of the compounds excreted into the medium.
Martinelli
D
D
University of Zurich, Institute of Plant Biology, Zollikerstrasse 107, Switzerland.
Bachofen
R
R
Brandl
H
H
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis*; D018441:Biofilms; D002861:Chromobacterium / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*
D001426:Bacterial Proteins; D003470:Culture Media; D011135:Polysaccharides, Bacterial; C088386:homoserine lactone; D015107:4-Butyrolactone