Initial proteome analysis of mature barley seeds and malt.
Several barley (Hordeum vulgare) cultivars are used in the production of malt for brewing. The malt quality depends on the cultivar, its growth and storage conditions, and the industrial process. To enhance studies on malt quality, we embarked on a proteome analysis approach for barley seeds and malt. The proteome analysis includes two-dimensional (2-D) gel electrophoresis, mass spectrometry, and bioinformatics for identification of selected proteins. This project initially focused on proteins in major spots in the neutral isoelectric point range (pI 4-7) including selected spots that differ between four barley cultivars. The excellent malting barley cultivar Barke was used as reference. Cultivar differences in the 2-D gel spot patterns are observed both at the seed and the malt level. In seed extracts one of the proteins causing variations has been identified as an alpha-amylase/trypsin inhibitor. In malt extracts multiple forms of the alpha-amylase isozyme 2 have been identified in varying cultivar characteristic spot patterns. The present identification of proteins in major spots from 2-D gels includes 27 different proteins from 42 spots from mature seed extract, while only three specific proteins were identified by analysing 13 different spots from the corresponding malt extract. It is suggested that post-translational processing causes the same protein to occur in different spots.
Østergaard
Ole
O
Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark.
Melchior
Sabrina
S
Roepstorff
Peter
P
Svensson
Birte
B
D019295:Computational Biology / Q000379:methods*; D002523:Edible Grain / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D001467:Hordeum / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D010940:Plant Proteins / Q000032:analysis*; D020033:Protein Isoforms; D020543:Proteome / Q000737:chemistry*; D016622:Silver Staining; D013045:Species Specificity; D000516:alpha-Amylases / Q000737:chemistry
D010940:Plant Proteins; D020033:Protein Isoforms; D020543:Proteome; D000516:alpha-Amylases