Liquid chromatographic method for determination of piperine in rat plasma: application to pharmacokinetics.
Piperine, a major alkaloid of Piper longum and Piper nigrum has been reported to have several pharmacological/toxicological effects. Though a number of methods for analysis of this omnipresent food component in pepper fruits are available, its analysis in body fluids has been largely neglected. A high-performance liquid chromatography method for the analysis of piperine in rat plasma is presented in this communication. Analysis was performed using a Symmetry C(18) column (250x4.6 mm) by isocratic elution with 25 mM KH(2)PO(4) (pH 4.5)-acetonitrile (35:65) and UV detection at 340 nm. The calibration plot was linear over the range studied (2-2000 ng) with correlation coefficient of 0.9984. Limit of detection and limit of quantitation were 1 ng/ml and 3 ng/ml, respectively. Good overall recovery (85.5+/-6%) was obtained with 4 ml ethyl acetate and extraction time of 3 min. Intra- and inter-assay coefficient of variation was found to be less than 7.5%. Plasma concentration-time profile of piperine in a conscious rat implanted with jugular vein cannula was obtained using this method. The method is simple, sensitive and reproducible.
Pharmacology Division, Regional Research Laboratory, Canal Road, Jammu 180 001, India. sbajad@yahoo.com
Sunil
S
Bajad
A K
AK
Singla
K L
KL
Bedi
D000470:Alkaloids; D000818:Animals; D052117:Benzodioxoles; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010880:Piperidines / Q000097:blood* / Q000493:pharmacokinetics; D053284:Polyunsaturated Alkamides; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D000470:Alkaloids; D052117:Benzodioxoles; D010880:Piperidines; D053284:Polyunsaturated Alkamides; C008922:piperine