[Culture and pluripotentiality of human marrow mesenchymal stem cells].
OBJECTIVE
To cultivate human mesenchymal stem cells (MSC) derived from fetal bone marrow and examine their pluripotentiality.


METHODS
Bone marrow mononuclear cells from human fetal femur were collected by Percoll gradient centrifugation. The low density cells including MSCs were cultivated and expanded in MSCGM media. The characteristics of the multipotent MSCs were observed by implanting them into nude mice for 4 weeks.


RESULTS
Human fetal marrow MSCs were successfully cultivated and differentiated in vivo into many kinds of tissues such as bone, cartilage, adipose, skeletal muscle and tendon-like tissue.


CONCLUSIONS
Human fetal marrow MSCs were multipotent stem cells.
Central Laboratory, The First Military Medical University, Guangzhou 510515, China.
Wenyu
W
Fu
Yanmeng
Y
Lu
Yingjie
Y
Piao
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002455:Cell Division; D017690:Cell Transplantation / Q000379:methods; D002478:Cells, Cultured; D006801:Humans; D008648:Mesoderm / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D013234:Stem Cells / Q000166:cytology*; D014183:Transplantation, Heterologous