[Geobacillus uralicus, a new species of thermophilic bacteria].
The K2T strain of thermophilic spore-forming bacteria was isolated from a biofilm on the surface of a corroded pipeline in an extremely deep well (4680 m, 40-72 degrees C) in the Ural. The cells are rod-shaped, motile, gram-variable. They grow on a complex medium with tryptone and yeast extract and on a synthetic medium with glucose and mineral salts without additional growth factors. The cells use a wide range of organic substances as carbon and energy sources. They exhibit a respiratory metabolism but are also capable of anaerobic growth on a nitrate-containing medium and of fermentation. Growth occurs within the 40-75 degrees C temperature range (with an optimum of 65 degrees C) and at pH 5-9. The minimum generation time (15 min) was observed at pH 7.5. Ammonium salts and nitrates are used as nitrogen sources. The G + C content of the DNA is 54.5 mol%. From the morphological, physiological, and biochemical properties and the nucleotide sequence of the 16S rRNA gene, it was concluded that the isolate K2T represents a new species of the genus Geobacillus, Geobacillus uralicus.
Popova
N A
NA
Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312 Russia.
Nikolaev
Iu A
IuA
Turova
T P
TP
Lysenko
A M
AM
Osipov
G A
GA
Verkhovtseva
N V
NV
Panikov
N S
NS
D000693:Anaerobiosis; D001482:Base Composition; D001483:Base Sequence; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004725:Endospore-Forming Bacteria / Q000145:classification* / Q000166:cytology / Q000502:physiology; D004783:Environmental Microbiology; D006863:Hydrogen-Ion Concentration; D009566:Nitrates; D010802:Phylogeny; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012426:Russia; D013696:Temperature
D003470:Culture Media; D004269:DNA, Bacterial; D009566:Nitrates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S