Chiral separation of methoxamine and lobeline in capillary zone electrophoresis using ethylbenzene-deactivated fused-silica capillary columns and cyclodextrins as buffer additives.
The complete chiral separation of methoxamine and lobeline was achieved by capillary zone electrophoresis on an ethylbenzene-deactivated fused-silica capillary column and with cyclodextrins (CDs) as buffer additives. Among the CDs investigated in this study, i.e. alpha-CD, beta-CD, dimethyl-beta-CD, hydroxypropyl-beta-CD and gamma-CD, all the three beta-type CDs showed chiral recognition on the two drugs investigated. Under the investigated conditions, the baseline chiral separation of methoxamine can be achieved with 90 mM Tris-H3PO4 (pH 2.5) containing 11.5 mM of the three beta-type CDs, with dimethyl-beta-CD giving the best resolution, whereas the baseline chiral separation of lobeline can be realized by using 90 mM Tris-H3PO4 buffer (pH 2.5) containing 5.8 mM dimethyl-beta-CD or 29.5 mM hydroxypropyl-beta-CD.
Russo
M V
MV
Facoltà di Agraria (DISTAAM), Università del Molise, Via de Sanctis, 86100, Campobasso, Italy. mvrusso@unimol.it
D001555:Benzene Derivatives / Q000737:chemistry; D002021:Buffers; D003505:Cyclodextrins / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D007202:Indicators and Reagents; D008120:Lobeline / Q000302:isolation & purification*; D008729:Methoxamine / Q000302:isolation & purification*; D013237:Stereoisomerism
D001555:Benzene Derivatives; D002021:Buffers; D003505:Cyclodextrins; D007202:Indicators and Reagents; D008120:Lobeline; D008729:Methoxamine; C004912:ethylbenzene