Constructing plant radiation hybrid panels.
Radiation hybrid (RH) mapping, a somatic cell genetic technique, has been developed in animal systems as a general approach for the construction of long-range physical maps of chromosomes. This statistical method relies on X-ray induced breakage of chromosomes to determine the physical distance between markers, as well as their order on the chromosome. The method can be applied to single chromosomes or across the whole genome. The generation of plant (barley) radiation hybrids and their culture in vitro is described here. PCR-based marker systems are used to verify hybrid status and to demonstrate genome coverage. RH panels of the type generated can be used for physical mapping, map-based cloning, or sequence contig assembly. RH resources will greatly aid the physical characterisation of crop plants with large genomes.
Wardrop
Julie
J
Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK.
Snape
John
J
Powell
Wayne
W
Machray
Gordon C
GC
D002478:Cells, Cultured; D018745:Genome, Plant; D001467:Hordeum / Q000235:genetics*; D021101:Radiation Hybrid Mapping / Q000379:methods*; D015203:Reproducibility of Results