Writing with DNA and protein using a nanopipet for controlled delivery.
We present a new, general method for the controlled deposition of biological molecules on surfaces, based on a nanopipet operating in ionic solution. The potential applied to the pipet tip controls the flux of biological molecules from the pipet, allowing fine control of the delivery rate. We used the ion current to control the distance of the pipet from the surface of a glass slide and deposited the fluorescently labeled DNA or protein G at a defined location onto the surface. Features of 830 nm size were obtained by depositing the biotinylated DNA onto a streptavidin surface; 1.3 mum size spots were obtained by depositing protein G onto a positively charged glass surface.
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
Andreas
A
Bruckbauer
Liming
L
Ying
Alison M
AM
Rothery
Dejian
D
Zhou
Andrew I
AI
Shevchuk
Chris
C
Abell
Yuri E
YE
Korchev
David
D
Klenerman
D004277:DNA, Single-Stranded / Q000737:chemistry*; D005453:Fluorescence; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000737:chemistry*
D004277:DNA, Single-Stranded; C029152:G-substrate; D009419:Nerve Tissue Proteins