The crystal structure of anthranilate phosphoribosyltransferase from the enterobacterium Pectobacterium carotovorum.
The structure of anthranilate phosphoribosyltransferase from the enterobacterium Pectobacterium carotovorum has been solved at 2.4 A in complex with Mn(2+)-pyrophosphate, and at 1.9 A without ligands. The enzyme structure has a novel phosphoribosyltransferase (PRT) fold and displays close homology to the structures of pyrimidine nucleoside phosphorylases. The enzyme is a homodimer with a monomer of 345 residues. Each monomer consists of two subdomains, alpha and alpha/beta, which form a cleft containing the active site. The nature of the active site is inferred from the trapped MnPPi complex and detailed knowledge of the active sites of nucleoside phosphorylases. With the anthranilate (An)PRT structure solved, the structures of all the enzymes required for tryptophan biosynthesis are now known.
Department of Chemistry/Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA.
Choel
C
Kim
Nguyen Huu
NH
Xuong
Steven
S
Edwards
Madhusudan
Muh Ching
MC
Yee
Glen
G
Spraggon
Stanley E
SE
Mills
D000595:Amino Acid Sequence; D000877:Anthranilate Phosphoribosyltransferase / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D019281:Dimerization; D011756:Diphosphates / Q000737:chemistry / Q000378:metabolism; D004755:Enterobacteriaceae / Q000201:enzymology*; D008345:Manganese / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010430:Pentosyltransferases / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D057845:Pyrimidine Phosphorylases; D013939:Thymidine Phosphorylase / Q000737:chemistry; D014364:Tryptophan / Q000096:biosynthesis* / Q000378:metabolism
D011756:Diphosphates; D008345:Manganese; D014364:Tryptophan; D010430:Pentosyltransferases; D057845:Pyrimidine Phosphorylases; D000877:Anthranilate Phosphoribosyltransferase; D013939:Thymidine Phosphorylase