Isolation of a putative laminin binding protein from Streptococcus anginosus.
Viridans streptococci, including Streptococcus anginosus, are a common cause of infective endocarditis in humans. Adherence mechanisms involved in colonization of non-diseased native valves (present in 40% of native valve endocarditis) are unknown. We have previously shown that an endocarditis isolate of S. anginosus adheres to exposed basement membrane of human and porcine valve tissue in a laminin dependent manner. We now describe the partial purification of an 80 kDa putative laminin binding protein (PLBP) by biochemical methods. Amino acid sequence of PLBP peptides is similar to substrate binding proteins of ABC transporters in other Gram-positive cocci.
Indianapolis VA Medical Center and Department of Internal Medicine, Division of Infectious Diseases, Indiana University School of Medicine, Indianapolis, Indiana, USA. bradalle@iupui.edu
Bradley L
BL
Allen
Magnus
M
Höök
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D001422:Bacterial Adhesion / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D004697:Endocarditis, Bacterial / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017474:Receptors, Laminin / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D034366:Streptococcus anginosus / Q000378:metabolism* / Q000502:physiology
D000906:Antibodies; D001426:Bacterial Proteins; C447144:Lbp protein, Streptococcus; D017474:Receptors, Laminin