Translocation of protein kinase C by halothane in cholinergic cells.
Protein kinase C (PKC) is a signal transducing enzyme that is an important regulator of multiple physiologic processes and a potential molecular target for volatile anaesthetic actions. However, the effects of these agents on PKC activity are not yet fully understood. Volatile anaesthetics increase intracellular calcium concentration ([Ca(2+)](i)) in a variety of cells, thus their effects on PKC activity may be indirect due to [Ca(2+)](i) increase. Alternatively, the anaesthetics could directly stimulate PKC activity. In order to distinguish these two possibilities in intact cells, we used a fully functional green fluorescent protein conjugated PKCbetaII (GFP-PKCbetaII) and confocal microscopy to evaluate the dynamic redistribution of PKC in living SN56 cells, a cholinergic cell line, in response to halothane. Halothane induced PKC translocation in SN56 cells transfected with GFP-PKCbetaII. This effect was not suppressed by dantrolene, a drug that blocks halothane-induced Ca(2+) release from intracellular stores in these cells. These findings indicate that halothane induces PKC translocation in SN56 cells independently of its ability to release calcium from internal stores.
Gomez
R S
RS
Departamento de Cirurgia, Faculdade de Medicina da UFMG, Belo Horizonte-Minas Gerais, Brazil. renatogomez@ig.com.br
Barbosa
J
J
Guatimosim
C
C
Massensini
A R
AR
Gomez
M V
MV
Prado
M A M
MA
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002799:Cholinergic Fibers / Q000187:drug effects / Q000201:enzymology*; D003620:Dantrolene / Q000494:pharmacology; D049452:Green Fluorescent Proteins; D006221:Halothane / Q000494:pharmacology*; D007202:Indicators and Reagents / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics; D018613:Microscopy, Confocal; D009125:Muscle Relaxants, Central / Q000494:pharmacology; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D064546:Protein Kinase C beta
D018685:Anesthetics, Inhalation; D007202:Indicators and Reagents; D007527:Isoenzymes; D008164:Luminescent Proteins; D009125:Muscle Relaxants, Central; D049452:Green Fluorescent Proteins; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D003620:Dantrolene; D002118:Calcium; D006221:Halothane