Characteristics of BmK AS Binding to Rat Brain and Cockroach Nerve Cord Synaptosomes.
BmK AS, one novel neurotoxic polypeptide from Chinese scorpion Buthus martensi Karsch was labeled by (125)I with INDOGEN method. Iododerivative was used in binding experiments on mammal and insect synaptosomes. BmK AS binds specifically to a single class of non-interacting binding sites with high affinity (K(d)=1.49 nmol/L) and low capacity (B(max)=1.39 nmol/g) on rat brain synaptosomes. Likewise, BmK AS also binds specifically to a single class of non-interacting binding sites with high affinity (K(d)=0.79 nmol/L) and low capacity (B(max)=6.60 nmol/g) on cockroach nerve cord synaptosomes. It indicates that there are special target receptors for BmK AS type scorpion neurotoxins on both mammal and insect excitable cell membranes.
Shanghai Institute of Physiology, Shanghai Research Center of Life sciences, the Chinese Academy of Sciences, Shanghai 200031, China. yhji@server.shcnc.ac.cn
Ya-Jun
YJ
Li
Yan
Y
Liu
Yong-Hua
YH
Ji