Inducing precocious anaphase in cultured mammalian cells.
The spindle checkpoint, which prevents anaphase onset upon spindle damage or incorrect chromosome alignment, presents a problem for experimental analysis of protein function in anaphase and cytokinesis. This is because the functional disruption of many proteins before anaphase onset can activate this checkpoint, preventing anaphase and subsequent cell cycle events. This paper compares new and old methods of overriding the spindle checkpoint in prometaphase mammalian tissue culture cells.
Canman
Julie C
JC
University of North Carolina, Department of Biology, 607 Fordham Hall, CB# 3280, Chapel Hill, NC 27599-3280, USA. jccanman@email.unc.edu
Salmon
E D
ED
Fang
Guowei
G
D000705:Anaphase; D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002478:Cells, Cultured; D005656:Fungal Proteins / Q000235:genetics; D008322:Mammals / Q000033:anatomy & histology / Q000378:metabolism; D008845:Microinjections; D008957:Models, Genetic; D009687:Nuclear Proteins; D010750:Phosphoproteins / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; C501384:MAD1L1 protein, human; C501385:Mad1l1 protein, mouse; D009687:Nuclear Proteins; D010750:Phosphoproteins; D012097:Repressor Proteins