Cloning and characterization of temperature-related gene TRS1.
To investigate the mechanism of spermatogenesis arrest derived from heat treatment and to screen temperature-related genes involved in spermatogenesis, the authors analyzed the differences in gene expression between cryptorchid and scrotal testes in rats, and cloned a full-length cDNA named TRS1. In situ hybridization showed that TRS1 mRNA was mainly expressed in spermatocyte and round spermatids in testis. The expression level decreased in cryptorchid testis, suggesting that the lower scrotal temperature is a key factor in keeping the normal expression of TRS1. At the N-terminal of TRS1, there was a plecstrin homology (PH) domain signature. This PH domain has high similarity to that in PEPP2, a homosapien protein, which has a characteristic of binding phosphatidylinositol 3-phosphate via its PH domain in vitro. These findings suggest that TRS1 may be important in spermatogenesis and give clues for further research on the function of TRS1.
Han
X-B
XB
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.
Zhou
X-C
XC
Hu
Z-Y
ZY
Zhang
Z-H
ZH
Liu
Y-X
YX
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D001667:Binding, Competitive; D003001:Cloning, Molecular; D003456:Cryptorchidism / Q000378:metabolism; D015870:Gene Expression; D006358:Hot Temperature; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012611:Scrotum / Q000378:metabolism; D016415:Sequence Alignment; D013091:Spermatogenesis; D013094:Spermatozoa / Q000737:chemistry; D013737:Testis / Q000737:chemistry / Q000378:metabolism
C463780:Plekha5 protein, rat; D011506:Proteins; D012333:RNA, Messenger