[Relationship between human parvovirus B19 infection and spontaneous abortion and congenital heart disease in China].
OBJECTIVE
To explore the status of human parvovirus B19(HPV B19) infection to the fetus in China and the relationship between HPV B19 infection and spontaneous abortion,and congenital heart disease (CHD).


METHODS
Nest polymerase chain reaction (PCR) and in situ hybridization (ISH) were used to detect HPV B19-DNA in the tissues of spontaneous abortion and cardiac muscle of CHD, enzyme linked immunosorbent assay (ELISA) to detect HPV B19-IgM.


RESULTS
(1) The positive rate of HPV B19-DNA was 25.8%(47/182) in spontaneous abortion, comparing with artificial abortion 5.0%(2/40). (2) The positive rate in cardiac muscle tissues of CHD in autopsy and biopsy were 17.2%(5/29) and 18.9%(7/37), respectively. The total rate was 18.2% (12/66), but the positive case was not found in control group (0/30). The HPV B19 gene were found locating in the nucleus of cardiac cell by ISH. The HPV B19-IgM was negative in the blood in all patients when the HPV B19-DNA positive in the cardiac cell of biopsy.


CONCLUSIONS
(1)There may be a relationship between HPV B19 infection and spontaneous abortion and CHD. (2) Two spontaneous abortion specimens from one person in the different period were both positive. It indicated that, there was a persistent infection. It may be one cause in frequently abortion.
Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xi' an 710032, China.
Dongliang
D
Xu
Guocheng
G
Zhang
Biao
B
Li
Xiaoming
X
Wang
Xin
X
Sun
D000022:Abortion, Spontaneous / Q000209:etiology / Q000821:virology*; D000328:Adult; D004279:DNA, Viral / Q000097:blood; D004622:Embryo, Mammalian / Q000821:virology; D005260:Female; D006330:Heart Defects, Congenital / Q000209:etiology / Q000821:virology*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D009206:Myocardium / Q000473:pathology; D010322:Parvoviridae Infections; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*
D004279:DNA, Viral; D007075:Immunoglobulin M