Identification of monoclonal antibodies that cross-react with cytokines from different animal species.
Eleven monoclonal antibodies specific for ovine, bovine and human cytokines were investigated by flow cytometry for cross-reactivities with cytokines produced by peripheral blood mononuclear cells (PBMCs) from sheep, cattle, goat, swine, horse, dog, mink, rabbit and human. Four antibodies specific for IL-4, IL-8, IFN-gamma and TNF-alpha cross-reacted with cytokines from a majority of the species investigated. These antibodies can be applied to flow cytometric studies of cytokine production by PBMCs from several veterinary species. Another five antibodies specific for IL-2, IL-6, GM-CSF and IFN-gamma (two antibodies) cross-reacted weakly and with a variable number of animal species. These antibodies could in certain situations be useful in flow cytometry. In a number of cases the immunological cross-reactivities were confirmed by Western blot analyses. Overall, the results of this study will remedy some of the lack of species-specific anti-cytokine antibodies in veterinary research.
Department of Veterinary Microbiology, Immunological Laboratory, The Royal Veterinary and Agricultural University, Stigbojlen 7, DK-1870 Frederiksberg C, Copenhagen, Denmark.
L G
LG
Pedersen
Y
Y
Castelruiz
S
S
Jacobsen
B
B
Aasted
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D002478:Cells, Cultured; D003429:Cross Reactions; D016207:Cytokines / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D016207:Cytokines