Clinical evaluation of oxidative stress and myocardial reperfusion injury in pediatric cardiac surgery.
BACKGROUND
To evaluate oxidative stress and myocardial damage after aortic crossclamping release (ACCR) during cardio pulmonary bypass (CPB) in children two parameters were investigated: total glutathione (GSH) and its oxidoreductive reactions (GSH/GSSG) as expression of oxidative stress, and plasmatic turnover of myocardial taurine (TAU) as expression of cell damage.


METHODS
The study was divided in two periods: 1) first period: analysis of oxidative stress and myocardial damage in 18 children. 2) Second period: evaluation of myocardial cell protection by controlled anterograde low oxygen warm reperfusion (ALOWR) before declamping. Twenty-one children were divided in two groups: not receiving (Group 1, 9 patients) and receiving (Group 2, 12 patients) ALOWR.


RESULTS
In the first period GSH values increased significantly after onset of mechanical ventilation (MV) in vein, after CPB start in artery and after ACCR in coronary sinus. Moreover TAU turnover in aortic and coronary sinus blood increased significantly after ACCR. In the second period, Group 2 showed a lower oxidative stress after ACCR, while no differences were observed in TAU turnover.


CONCLUSIONS
1) Assessment of TAU and GSH levels can be considered a good method to clinically evaluate myocardial injury during cardiac surgery. 2) MV and CPB can induce oxidative stress before aortic clamping and can decrease the physiologic scavengers. Therefore, to prevent that depletion, the strategy of these techniques must be adapted to the patient and to his cardiac disease. 3) Intramyocardial TAU turnover is not significantly modified by the reperfusion technique. 4) ALOWR can reduce myocardial oxidative stress and can improve heart recovery after the cardioplegic arrest.
Calza
G
G
Department of Cardiovascular Surgery, Giannina Gaslini Institute, Children's Hospital, Genoa, Italy. giovannicalza@ospedale-gaslini.ge.it
Lerzo
F
F
Perfumo
F
F
Borini
I
I
Panizzon
G
G
Moretti
R
R
Grasso
P
P
Virgone
A
A
Zannini
L
L
D002315:Cardiopulmonary Bypass; D016022:Case-Control Studies; D002648:Child; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000175:diagnosis*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress; D012121:Respiration, Artificial; D013654:Taurine / Q000378:metabolism; D013997:Time Factors
D013654:Taurine; D005978:Glutathione