Transcriptional activation of the human insulin receptor gene by 1,25-dihydroxyvitamin D(3).
Treatment with 10(-8) M 1,25-dihydroxyvitamin D(3) for 24 h causes transcriptional activation of the human insulin receptor gene in U-937 human promonocytic cells. The activation seems to potentiate the response to insulin in terms of glucose oxidation. Wortmannin, a phosphatidylinositol 3-kinase inhibitor, causes a greater inhibition of insulin-stimulated glucose oxidation in 1,25-dihydroxyvitamin D(3)-treated cells than in untreated cells. This suggests a stimulation of phosphatidylinositol 3-kinase activity by 1,25-dihydroxyvitamin D(3), which could mediate, at least in part, the potentiation of the insulin response.
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Department of Biochemistry and Molecular Biology, Faculty of Medicine, Complutense University of Madrid, Spain.
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D000730:Androstadienes / Q000494:pharmacology; D002117:Calcitriol / Q000494:pharmacology*; D002120:Calcium Channel Agonists / Q000494:pharmacology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011972:Receptor, Insulin / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation; D014162:Transfection; D020298:U937 Cells; D000077191:Wortmannin
D000730:Androstadienes; D002120:Calcium Channel Agonists; D004791:Enzyme Inhibitors; D007328:Insulin; D008156:Luciferases; D011972:Receptor, Insulin; D002117:Calcitriol; D005947:Glucose; D010100:Oxygen; D000077191:Wortmannin