[The effects of antioxidants on pyridinoline cross linkage formation in human fibroblasts culture from hypertrophic scars].
OBJECTIVE
To investigate the effects of antioxidants on the pyridinoline crosslink formation in human fibroblasts culture from hypertrophic scars. The role of these antioxidants in reducing the level of pyridinoline crosslinks in-vitro without damaging the cells has important implications. These antioxidants can be applied for the treatment of hypertrophic scars.


METHODS
9 cases of hyprotrophic scar biopsies were taken from burn patients. The specimens were explant culture for 7 days. Insulated fibroblasts from these explant cultures were subcultured for 8 passages. At the last passage, the cultures were subjected to a free radical generating system and were added different antioxidants. The supernatant and cells were collected after 4 hrs of incubation and the concentration of pyridinoline crosslinks was determined using Pyrilink kit.


RESULTS
When the fibroblast cultures were subjected to free radicals and antioxidants, all samples showed a decrease in pyridinoline crosslinks concentration. The more potent antioxidants found in this study were catalase, superoxide dismutase and desferrioxamine. Particularly catalase which did not damage the cells.


CONCLUSIONS
The antioxidants used in this study were found to be effective in reducing the concentration of pyridinoline crosslinks. The best agent is catalase, which did not damage the cells and could be considered for clinical use.
Department of Orthopaedics & Traumatology, Chinese University of Hong Kong, Hong Kong, China.
Haytong
H
Wu
Leungkim
L
Hung
Pingchung
P
Leung
Koonching
K
Wan
D000293:Adolescent; D000328:Adult; D000596:Amino Acids / Q000378:metabolism*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D017439:Cicatrix, Hypertrophic / Q000473:pathology*; D003432:Cross-Linking Reagents / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008297:Male
D000596:Amino Acids; D000975:Antioxidants; D003432:Cross-Linking Reagents; C015484:pyridinoline; D002374:Catalase