Cross-reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro.
Cell characterization of primary cultures in vertebrates is well established but not in marine invertebrates. This fact is hampering advances in the development of tissue cultures from this species. In the present study, a panel of antibodies to structural proteins, stress proteins, oncogenes and proliferation antigens, developed against mammalian antigens, were tested in paraffin sections of the crustacean Pandalus borealis tissues. Several tissues were analysed: hepatopancreas, gills, ovaries, epithelium under the cuticle and abdominal muscle. Specific antibodies to crustacean proteins are not commercially available. The immunocytochemical results show that antibodies to human epitopes cross-react with antigens in the crustacean Pandalus borealis indicating that some cellular proteins are highly conserved in evolution. Cytokeratin, proliferating cell nuclear antigen, ras and p-glycoprotein were detected by immunocytochemistry in Pandalus borealis. No immunoreactivity for Ki-67 and metallothionein was observed. This system can help in validation and characterization of invertebrate cultures.
Lyons-Alcantara
Maria
M
Department of Radiation and Environmental Science, Radiation Science Centre, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland. maria.lyons@marine.ie
Lambkin
Helen A
HA
Nordmo
Rolf
R
Lyng
Fiona
F
Mothersill
Carmel
C
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000818:Animals; D033364:Decapoda / Q000033:anatomy & histology* / Q000276:immunology; D004064:Digestive System / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology; D000939:Epitopes / Q000737:chemistry*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D008668:Metallothionein / Q000378:metabolism; D009865:Oocytes / Q000166:cytology; D010053:Ovary / Q000166:cytology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D018631:ras Proteins / Q000378:metabolism
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000939:Epitopes; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D007633:Keratins; D008668:Metallothionein; D018631:ras Proteins