Spectroscopic and voltammetric study on the binding of aluminium(III) to DNA.
Cyclic voltammetry (CV) and ultraviolet (UV) spectroscopy were used, for the first time, to study the interaction between aluminium(III) and calf thymus DNA under neutral pH conditions. Thus obtained data confirmed the existence of a relatively strong interaction between Al(III) and DNA. The binding site for aluminium(III) on DNA chains is not the bases, but the phosphate groups on the DNA backbones, the same as that for [Co(phen)3](3+/2+) that binds non-specifically and electrostatically to the deoxyribose phosphate backbone of DNA. When coexisting, Al(III) binds more favorably to DNA than [Co(phen)3](3+/2+), which implies the relatively strong binding of Al(III) to the phosphate backbone of DNA under neutral pH conditions. The nature of the binding of Al(III) to DNA is also discussed.
Zhang
Rong-Ying
RY
Department of Chemistry, Wuhan University, PR China.
Liu
Yi
Y
Pang
Dai-Wen
DW
Cai
Ru-Xiu
RX
Qi
Yi-Peng
YP
D000535:Aluminum / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003217:Conductometry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D015341:Nucleic Acid Probes / Q000737:chemistry / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013950:Thymus Gland
D015341:Nucleic Acid Probes; D004247:DNA; D000535:Aluminum