Determination of Mn(II) and Co(II) with Arsenazo III.
Complex formation between Arsenazo III and Mn2+ and Co2+ at equilibrium has been investigated at pH 7.2, and the stoichiometry and stability of the complexes have been determined. The data indicate that Arsenazo III is suitable for determination of Mn2+ and Co2+ on the micromolar scale. The dissociation constants of the phosphate complexes of Mn2+ and Co2+ at pH 7.2 were estimated with Arsenazo III as 3.6 and 10 mM, respectively.
Belozersky Institute of Physico-Chemical Biology and School of Chemistry, Lomonosov Moscow State University, Moscow, 119992, Russia.
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Zyryanov
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Baykov
D001150:Arsenazo III / Q000737:chemistry*; D003035:Cobalt / Q000032:analysis* / Q000737:chemistry; D007202:Indicators and Reagents; D007700:Kinetics; D008345:Manganese / Q000032:analysis* / Q000737:chemistry; D011755:Pyrophosphatases / Q000032:analysis; D013053:Spectrophotometry
D007202:Indicators and Reagents; D001150:Arsenazo III; D003035:Cobalt; D008345:Manganese; D011755:Pyrophosphatases