Uptake of cadmium ions in white-rot fungus Trametes versicolor: effect of cd (II) ions on the activity of laccase.
The effects of cadmium Cd (II) ions on the physiology and biological activity of Trametes versicolor, a strain belonging to white-rotting Basidiomycetes, were examined. Cd (II) ions were added to 10-day-old cultures grown on a liquid medium, or at the time of inoculation. Our experiments showed that T. versicolor is a good cadmium biosorbent from aqueous solution, this strain removing almost all the Cd (ll) ions over the first 2h of incubation by what appears to be a rapid, energy-independent surface binding phenomenon, at the rate of approximately 2mg Cd per g mycelial dry weight. An additional slower and energy-dependent transport mechanism was also present, taking in approximately 0.3mg Cd (II) perg dry weight. It is also shown that these Cd (II) ions significantly stimulate the activity of extracellular laccase when added to 10-day-old cultures.
Department of Biochemistry, Maria Curie-Sklodowska University, Sklodowska Square 3, 20-031, Lublin, Poland.
Anna
A
Jarosz-Wilkolazka
Elzbieta
E
Malarczyk
Jacek
J
Pirszel
Tadeusz
T
Skowroński
Andrzej
A
Leonowicz
D000327:Adsorption / Q000187:drug effects; D001487:Basidiomycota / Q000187:drug effects / Q000201:enzymology*; D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D002104:Cadmium / Q000378:metabolism / Q000493:pharmacokinetics* / Q000633:toxicity; D019256:Cadmium Chloride / Q000493:pharmacokinetics; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004781:Environmental Exposure / Q000009:adverse effects; D045604:Extracellular Fluid / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects* / Q000502:physiology; D042845:Laccase / Q000187:drug effects* / Q000378:metabolism
D002104:Cadmium; D042845:Laccase; D005947:Glucose; D019256:Cadmium Chloride