Tissue factor pathway inhibitor can interact with platelets.
Tissue factor pathway inhibitor (TFPI) regulates the extrinsic pathway of blood coagulation. However, there is no report on interaction between TFPI and platelets other than that by Tsuji, who found that whole blood anticoagulated with TFPI exhibited remarkable decrease in platelet count. Our study revealed that washed platelets suspended in modified Tyrode's buffer (8 mM CaCl2) containing TFPI exhibit platelet aggregation. However, platelets aggregation was observed without TFPI, but its increase and intensity were slow and weak, compared to that in the presence of TFPI. This aggregation was inhibited by anti-CD41 (anti-GPIIb) antibody. This finding suggested that TFPI promotes platelet aggregation.
Nishioka
Takuya
T
Department of Clinical and Laboratory Medicine, Osaka City University Medical School, 1-4-3 Asahimachi, Abeno, Osaka, Japan. taku.nishioka@nifty.ne.jp
Yokota
Masaharu
M
Hino
Masayuki
M
Tsuda
Izumi
I
Tatsumi
Noriyuki
N
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008074:Lipoproteins / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology
D000911:Antibodies, Monoclonal; D008074:Lipoproteins; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; C051928:lipoprotein-associated coagulation inhibitor