Alpha-N-acetylgalactosaminidase from marine bacterium Arenibacter latericius KMM 426T removing blood type specificity of A-erythrocytes.
An alpha-N-acetylgalactosaminidase IV able to remove blood type specificity of human A(II)-erythrocytes and not effecting B(III)-erythrocytes was isolated from the marine bacterium Arenibacter latericius KMM 426T. The alpha-N-acetylgalactosaminidase IV preparation exhibits high activity during inhibition of hemagglutination with blood group substance A containing determinants analogous to A-erythrocytes. The enzyme has a pH optimum from 7.0 to 8.0 and completely retains its activity during 30-min heating at 50 degrees C and for a week at 20 degrees C. The enzyme can be stored under the sterile conditions for any length of time at 4 degrees C, but it does not withstand freezing. The alpha-N-acetylgalactosaminidase is resistant to NaCl; for p-nitrophenyl-alpha-N-acetyl-D-galactosaminide, the Km is 0.38 mM. The molecular mass of the enzyme determined by gel filtration is 84 kD.
Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia.
I Yu
IY
Bakunina
R A
RA
Kuhlmann
L M
LM
Likhosherstov
M D
MD
Martynova
O I
OI
Nedashkovskaya
V V
VV
Mikhailov
L A
LA
Elyakova
D000017:ABO Blood-Group System / Q000276:immunology*; D001426:Bacterial Proteins / Q000378:metabolism*; D002240:Carbohydrate Sequence; D004912:Erythrocytes / Q000276:immunology*; D016946:Gram-Negative Aerobic Rods and Cocci / Q000201:enzymology*; D006596:Hexosaminidases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D048809:alpha-N-Acetylgalactosaminidase
D000017:ABO Blood-Group System; D001426:Bacterial Proteins; D006596:Hexosaminidases; C482722:NAGA protein, human; D048809:alpha-N-Acetylgalactosaminidase