Production of a Mycobacterium avium ssp. paratuberculosis purified protein derivative (PPD) and evaluation of potency in guinea pigs.
A Mycobacterium avium ssp. paratuberculosis purified protein derivative (PPD) was produced and the biologic activity evaluated in sensitized guinea pigs. The PPD when adjusted to a protein concentration of 1mg/ml induced a delayed-type hypersensitivity response comparable to USDA Johnin OT 133-8707.
Steadham
E M
EM
Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, U.S.A.
Martin
B M
BM
Thoen
C O
CO
D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D009162:Mycobacterium avium / Q000276:immunology* / Q000378:metabolism; D010283:Paratuberculosis / Q000175:diagnosis*; D012867:Skin / Q000378:metabolism; D013997:Time Factors; D014373:Tuberculin / Q000737:chemistry*
D001426:Bacterial Proteins; D014373:Tuberculin