Contribution of phospholipomannan to the surface expression of beta-1,2-oligomannosides in Candida albicans and its presence in cell wall extracts.
beta-1,2-Oligomannosides (beta-1,2-Man) derived from Candida albicans mannan have been shown to act as adhesins and to induce protective antibodies. We used monoclonal antibodies specific for beta-1,2-Man in electron, confocal, and fluorescence microscopy to study the surface expression of beta-1,2-Man epitopes. These monoclonal antibodies were also used for Western blotting of cell surface extracts to study the nature of the molecules expressing the beta-Man epitopes. Evidence was obtained for the contribution of a glycolipid, phospholipomannan (PLM), to the complex expression of beta-1,2-Man epitopes at the cell wall surfaces of yeasts grown on solid media. PLM was present in intercellular matrixes of colonies grown on agar and was detected as a contaminant in mannan batches prepared by conventional methods.
Poulain
D
D
Laboratoire de Mycologie Fondamentale et Appliquée, INSERM EPI 9915, Faculté de Médecine, Pôle Recherche, 59045 Lille Cedex, Italy. dan_pulain@compuserve.com
Slomianny
C
C
Jouault
T
T
Gomez
J M
JM
Trinel
P A
PA
D002176:Candida albicans / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002473:Cell Wall; D018985:Epitopes, B-Lymphocyte / Q000096:biosynthesis / Q000276:immunology; D006017:Glycolipids / Q000378:metabolism*; D008351:Mannans / Q000302:isolation & purification; D008623:Mercaptoethanol; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D016253:Microscopy, Immunoelectron / Q000379:methods; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000276:immunology
D018985:Epitopes, B-Lymphocyte; D006017:Glycolipids; D008351:Mannans; D009844:Oligosaccharides; C028569:oligomannoside; C400188:phospholipomannan; D008623:Mercaptoethanol