Cloning of Human Glial Neurotrophic Factor and Its High Expression in Insect Cells.
The human glial cell derived neurotrophic factor (GDNF) was expressed in Tn-5B1-4 insect cells using the Bac to Bac baculovirus expression system, accounting for 30% of total cellular soluble proteins. The expressed product was purified by affinity chromatography and the activity assays showed that it could significantly promote the survival of dopaminergic neurons. The study can be the basis for further structural-functional analysis of GDNF.
Chen
Zhe-Yu
ZY
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. xfwu@sunm.shcnc.ac.cn
Lu
Chang-Lin
CL
Sun
Jian-Xin
JX
Wu
Xiang-Fu
XF