Effects of bisphenol A and artificial UVB radiation on the early development of Rana temporaria.
Ultraviolet-B (UVB) radiation is one environmental factor that may act in concert with other stressors, such as xenobiotics, to produce adverse effect on amphibian populations. Embryos (< 24 h old) of the common frog (Rana temporaria) were exposed to four concentrations (0, 10, 100, or 1000 microg/L) of bisphenol A (BPA), with and without ultraviolet-B (UVB) radiation, for 20 d in the laboratory. Throughout the experiment the biologically effective daily UVB dose, calculated with Setlow's DNA action spectrum, was 2.80 kJ m(-2). UVB radiation as such did not have any significant effect on frog embryos. However, a BPA treatment of 1000 microg/L had a significant effect on embryos in both the UVB and no-UVB treatments, with the effect being greater with UVB. UVB produced a significant decrease in survival in the newly hatched frog larvae at all BPA concentrations. These results demonstrate that simultaneous exposure to these two stress factors is more harmful to R. temporaria larvae than exposure to one stressor alone.
Laboratory of Aquatic Ecology and Ecotoxicology, Department of Biology, University of Joensuu, Joensuu, Finland. petri.koponen@joensuu.fi
Petri S
PS
Koponen
Jussi V K
JV
Kukkonen
D000818:Animals; D001559:Benzhydryl Compounds; D000013:Congenital Abnormalities; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000528:radiation effects; D004785:Environmental Pollutants / Q000633:toxicity; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D007814:Larva / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D010636:Phenols / Q000633:toxicity*; D011896:Rana temporaria; D015996:Survival Rate; D014466:Ultraviolet Rays / Q000009:adverse effects*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds