Volume expansion suppresses the tooth-pulp evoked jaw-opening reflex related activity of trigeminal neurons in rats.
The aim of the present study is to clarify whether physiological stimulation of vagal afferents modulates the activity of the trigeminal spinal nucleus oralis (TSNO) neurons related to the tooth-pulp (TP)-evoked jaw-opening reflex (JOR) in pentobarbital-anesthetized rats. The activity of TSNO neurons and the amplitude of digastric electromyogram (dEMG) increased proportionally during 1.0-3.5 times the threshold for JOR. The amplitude of the dEMG of 14 out of 17 rats was suppressed by physiological stimulation of vagal afferents after intravenous infusion of Ficoll. Out of 23, 18 TSNO unit activities in 14 rats were also suppressed by Ficoll infusion. This suppressive effect of unit and dEMG activities returned to the control level within 25 min. After administration of naloxone (0.5 and 1.0 mg/kg, i.v.) the suppressive effect of Ficoll infusion on the activity of TSNO neurons (5/7) was significantly attenuated compared to the control (p < 0.01). The inhibition TSNO neuronal and dEMG activities by Ficoll infusion was volume-dependent in a range of 5-10% of total blood volume. Furthermore, right vagus nerve ligation greatly inhibited the suppressive effect of Ficoll-induced TSNO activity. These results therefore suggest that low-pressure cardiopulmonary baroreceptors whose afferents travel in the vagus nerve inhibit the pulpal nociceptive transmission.
Department of Physiology, School of Dentistry at Tokyo, Nippon Dental University, Tokyo, Japan. m-takeda@tokyo.ndu.ac.jp
Mamoru
M
Takeda
Takeshi
T
Tanimoto
Toshimi
T
Nishikawa
Mizuho
M
Ikeda
Shinki
S
Yoshida
Masahiro
M
Ito
Shigeji
S
Matsumoto
D000818:Animals; D003782:Dental Pulp / Q000294:innervation*; D004558:Electric Stimulation; D004576:Electromyography; D005157:Facial Pain / Q000503:physiopathology; D005362:Ficoll / Q000494:pharmacology; D007568:Jaw / Q000502:physiology*; D008026:Ligation; D008297:Male; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology; D051381:Rats; D012018:Reflex / Q000502:physiology*; D014279:Trigeminal Nucleus, Spinal / Q000166:cytology / Q000502:physiology*; D014630:Vagus Nerve / Q000166:cytology / Q000502:physiology*
D009292:Narcotic Antagonists; D005362:Ficoll; D009270:Naloxone