Brain proteasomal function in sporadic Parkinson's disease and related disorders.
Because genetic defects relating to the ubiquitin-proteasome system were reported in familial parkinsonism, we evaluated proteasomal function in autopsied brains with sporadic Parkinson's disease. We found that proteasome peptidase activities in a fraction specific to the proteasome were preserved in five brain areas (including the striatum) of Parkinson's disease where neuronal loss is not observed. Striatal protein levels of two proteasome subunits were normal in Parkinson's disease but reduced mildly in disease controls (multiple system atrophy). Our brain data suggest that a systemic, global disturbance in the catalytic activity and degradation ability of the proteasome itself is unlikely to explain the cause of Parkinson's disease.
Furukawa
Yoshiaki
Y
Movement Disorders Research Laboratory, Centre for Addiction and Mental Health-Clarke Division, 250 College Street, Toronto, Ontario, Canada M5T 1R8. yoshiaki_furukawa@camh.net
Vigouroux
Sophie
S
Wong
Henry
H
Guttman
Mark
M
Rajput
Ali H
AH
Ang
Lee
L
Briand
Marièle
M
Kish
Stephen J
SJ
Briand
Yves
Y
D000368:Aged; D001921:Brain / Q000201:enzymology* / Q000502:physiology; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism*; D019578:Multiple System Atrophy / Q000201:enzymology* / Q000235:genetics; D010300:Parkinson Disease / Q000201:enzymology* / Q000235:genetics; D010447:Peptide Hydrolases / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D013494:Supranuclear Palsy, Progressive / Q000201:enzymology* / Q000235:genetics
D009097:Multienzyme Complexes; D010447:Peptide Hydrolases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex