Effect of anti-CD14 antibody on experimental periodontitis induced by Porphyromonas gingivalis lipopolysaccharide.
The lipopolysaccharide (LPS) released by Porphyromonas gingivalis, a Gram-negative bacterium found in the periodontal pockets of patients with periodontitis, induces bone resorbing activity in vivo. We previously showed that a receptor for LPS on human gingival fibroblasts and gingival epithelial cells is CD14. In this study, we established a mouse model of experimental periodontitis by applying a P. gingivalis LPS solution to the buccal region of mice. P. gingivalis LPS-induced bone resorption and interleukin-6 production in the gingival tissues were significantly inhibited by pretreatment with anti-CD14 antibody for 5 weeks prior to LPS treatment. This result suggests that anti-CD14 antibody may be usable as a prototype for the development of drugs for the treatment of periodontal disease.
Department of Pharmacology, Osaka Dental University, Hirakata, Japan. ohhourei@po.mdu.ac.jp
Pao-Li
PL
Wang
Mari
M
Oido-Mori
Takeo
T
Fujii
Yusuke
Y
Kowashi
Masanori
M
Kikuchi
Yasushi
Y
Suetsugu
Junzo
J
Tanaka
Yasutaka
Y
Azuma
Mitsuko
M
Shinohara
Kiyoshi
K
Ohura
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000627:therapeutic use*; D018950:Lipopolysaccharide Receptors / Q000276:immunology*; D008070:Lipopolysaccharides / Q000378:metabolism*; D051379:Mice; D010518:Periodontitis / Q000378:metabolism / Q000382:microbiology / Q000628:therapy*; D016966:Porphyromonas gingivalis / Q000378:metabolism / Q000472:pathogenicity*
D000906:Antibodies; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides