Local proliferation of microglia cells in response to neocortical injury in vitro.
Studies examined whether increased numbers of microglia following neural damage result from induced mitotic activity of resident microglia in situ. Organotypic slice cultures of neocortex were maintained for 1 week prior to placement of lesions. Increased numbers of OX-6 or tomato lectin labeled microglial cells were detected 1-8 days following lesions. Administration of BrdU to the cultures demonstrated lectin and BrdU double labeled microglial cells, conclusively demonstrating that a portion of the microglial cells were generated by local mitotic activity in situ.
Department of Anatomy and Neurobiology, College of Medicine, University of California, Irvine, Irvine, CA , USA.
David A
DA
Eliason
Seth A
SA
Cohen
Janie
J
Baratta
Jen
J
Yu
Richard T
RT
Robertson
D000818:Animals; D000963:Antimetabolites; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology* / Q000293:injuries*; D017628:Microglia / Q000166:cytology*; D009924:Organ Culture Techniques; D010587:Phagocytosis / Q000502:physiology; D051381:Rats
D000963:Antimetabolites; D001973:Bromodeoxyuridine