Quantification of neuramidase (NA) protein content.
An ELISA-immunocapture assay was developed for quantification of influenza neuraminidases (N2 and B), with a sensitivity about 10 ng neuramidase(NA)/ml. This sensitive and specific assay was applied to several trivalent vaccine preparations (1997/1998 and 1998/1999). It was shown capable to detect 3.5-6.9 microg NA/ml according to the influenza strain and the vaccine origin.
Aymard
Michèle
M
Université Claude Bernarde, Faculte de Medecine, 8, Avenue Rockefeller, 69373 Lyon Cedex 08, France. aymard@rockefeller.univ-lyon1.fr
D004797:Enzyme-Linked Immunosorbent Assay; D009439:Neuraminidase / Q000032:analysis*; D012680:Sensitivity and Specificity
D009439:Neuraminidase