DJ-1, a target protein for an endocrine disrupter, participates in the fertilization in mice.
DJ-1 was first identified as an activated ras-dependent oncogene product and was later also found to be an infertility-related protein affected by sperm toxicants such as ornidazole (OR) and epichlorohydrin. These findings suggest that DJ-1 has functions in both somatic cells and sperm. In this study, to determine the relationship between DJ-1 and an endocrine disrupter and to determine the functions of DJ-1 in sperm, in vitro fertilization experiments were carried out using eggs and sperm extracted from mice that had or had not been treated with OR. We found that the amount of DJ-1 in sperm and the efficiency of fertilization decreased with the increasing dose of OR to which the mice were exposed. The addition of an anti-mouse DJ-1 serum to sperm solution before the in vitro fertilization reaction with eggs resulted in a decrease in the efficiency of fertilization to about one-third of that when pre-immune serum was added to sperm solution, indicating that DJ-1 participates in the fertilization.
Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Masahiko
M
Okada
Ken-ichi
K
Matsumoto
Takeshi
T
Niki
Takahiro
T
Taira
Sanae M M
SM
Iguchi-Ariga
Hiroyoshi
H
Ariga
D000818:Animals; D000941:Antigens / Q000276:immunology; D005260:Female; D005306:Fertilization / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D005307:Fertilization in Vitro; D006801:Humans; D007106:Immune Sera; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D015513:Oncogene Proteins / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D009950:Ornidazole / Q000633:toxicity; D000071617:Protein Deglycase DJ-1; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*
D000941:Antigens; D007106:Immune Sera; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D009950:Ornidazole; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1