Visualization of advanced human prostate cancer lesions in living mice by a targeted gene transfer vector and optical imaging.
Non-invasive imaging and transcriptional targeting can improve the safety of therapeutic approaches in cancer. Here we demonstrate the ability to identify metastases in a human-prostate cancer model, employing a prostate-specific adenovirus vector (AdPSE-BC-luc) and a charge-coupled device-imaging system. AdPSE-BC-luc, which expresses firefly luciferase from an enhanced prostate-specific antigen promoter, restricted expression in the liver but produced robust signals in prostate tumors. In fact, expression was higher in advanced, androgen-independent tumors than in androgen-dependent lesions. Repetitive imaging over a three-week period after AdPSE-BC-luc injection into tumor-bearing mice revealed that the virus could locate and illuminate metastases in the lung and spine. Systemic injection of low doses of AdPSE-BC-luc illuminated lung metastasis. These results demonstrate the potential use of a non-invasive imaging modality in therapeutic and diagnostic strategies to manage prostate cancer.
Department of Urology, David Geffen School of Medicine at UCLA, Los Angeles California 90095, USA.
Jason Y
JY
Adams
Mai
M
Johnson
Makoto
M
Sato
Frank
F
Berger
Sanjiv S
SS
Gambhir
Michael
M
Carey
M Luisa
ML
Iruela-Arispe
Lily
L
Wu
D000818:Animals; D003952:Diagnostic Imaging; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008822:Mice, Transgenic; D009368:Neoplasm Transplantation; D017430:Prostate-Specific Antigen / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology* / Q000628:therapy; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013131:Spine / Q000473:pathology
D011993:Recombinant Fusion Proteins; D008156:Luciferases; D017430:Prostate-Specific Antigen