[Expression of inducible nitric oxide synthase and macrophage in human placenta of pregnancy induced hypertension syndrome].
OBJECTIVE
To investigate the expression of inducible nitric oxide synthase (iNOS) and macrophage (CD(68)) in the placenta of the patients with pregnancy induced hypertension syndrome(PIH) and the role of them in the pathogenesis of PIH.


METHODS
Immunohistochemical technique (SABC) was used for immunostaining and detecting inducible nitric oxide synthase and CD(68) in the placentas of thirty patients of PIH (two patients of mild PIH; seven moderate PIH; twenty-one severe PIH) and twenty-two normal pregnancies (the control group).


RESULTS
The level of inducible nitric oxide synthase and CD(68) in the placenta of patients of PIH were (2.9 +/- 0.7) % and (3.0 +/- 0.8) %, respectively and (2.1 +/- 0.8) % and (2.1 +/- 0.7) % in the control group. They were both significantly different (P < 0.01). The levels of inducible nitric oxide synthase in the mild moderate and severe PIH were (2.8 +/- 0.7) % and (3.0 +/- 0.7) %, and the CD(68) were (2.8 +/- 1.0) % and (3.1 +/- 0.8) %, which were not significantly different (P > 0.05).


CONCLUSIONS
The obvious increase of iNOS and macrophage in the placenta of patients of PIH can be observed and iNOS and CD(68) are involved in the immunization and inflammatory reaction. This may be one of the causes for human placental dysfunction and high impedance of fetoplacental circulation in PIH. It may play an important role in the genesis of pregnancy induced hypertension.
Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University Medical Collage, Qingdao 266003, China.
Jianxin
J
Zuo
Lingna
L
Zhang
Wenjie
W
Zeng
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D005260:Female; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000201:enzymology* / Q000209:etiology; D007150:Immunohistochemistry; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood; D008264:Macrophages / Q000201:enzymology* / Q000276:immunology; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D016173:Macrophage Colony-Stimulating Factor; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C496319:NOS2 protein, human