Peptide arrays for highly sensitive and specific antibody-binding fluorescence assays.
We report a novel generation of peptide arrays fabricated by site-specific ligation of glyoxylyl peptides onto glass slides covered by a semicarbazide sol-gel layer. These arrays allowed the highly sensitive and specific detection of antibodies in very small blood samples from infected individuals using three model peptidic epitopes (HCV Core and NS4, EBV Capsid) in an immunofluorescence assay. Comparison with standard enzyme-linked immunosorbent assays (ELISAs) demonstrated a large gain in sensitivity and specificity. These unique properties, combined with the possibility to immobilize glycoproteins such as antibodies, offer the possibility to perform sandwich immunofluorescent assays in a highly parallel format.
Melnyk
Oleg
O
UMR CNRS 8525, Biological Institute of Lille, 1 rue du Pr Calmette, 59021 Lille, France. oleg.melnyk@pasteur-lille.fr
Duburcq
Xavier
X
Olivier
Christophe
C
Urbès
Florence
F
Auriault
Claude
C
Gras-Masse
Hélène
H
D000914:Antibodies, Viral / Q000097:blood; D003432:Cross-Linking Reagents / Q000737:chemistry; D000939:Epitopes / Q000737:chemistry; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis; D015200:Fluoroimmunoassay / Q000379:methods* / Q000592:standards; D005898:Glass; D006526:Hepatitis C / Q000175:diagnosis; D018937:Hepatitis C Antibodies / Q000097:blood; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007159:Immunologic Tests / Q000379:methods / Q000592:standards; D008832:Microchemistry; D008904:Miniaturization; D010455:Peptides / Q000138:chemical synthesis*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D003432:Cross-Linking Reagents; D000939:Epitopes; D018937:Hepatitis C Antibodies; D010455:Peptides