Effect of ozone on bronchial mucosal inflammation in asthmatic and healthy subjects.
Epidemiological studies suggestthat asthmatics are more affected by ozone than healthy people. This study tested three hypotheses (1) that short-term exposure to ozone induces inflammatory cell increases and up-regulation of vascular adhesion molecules in airway lavages and bronchial tissue 6 h after ozone exposure in healthy subjects; (2) these responses are exaggerated in subjects with mild allergic asthma; (3) ozone exacerbates pre-existent allergic airways inflammation. We exposed 15 mild asthmatic and 15 healthy subjects to 0.2 ppm of ozone or filtered air for 2 h on two separate occasions. Airway lavages and bronchial biopsies were obtained 6 h post-challenge. We found that ozone induced similar increases in bronchial wash neutrophils in both groups, although the neutrophil increase in the asthmatic group was on top of an elevated baseline. In healthy subjects, ozone exposure increased the expression of the vascular endothelial adhesion molecules P-selectin and ICAM- 1, as well as increasing tissue neutrophil and mast cell numbers. The asthmatics showed allergic airways inflammation at baseline but ozone did not aggravate this at the investigated time point. At 6 h post-ozone-exposure, we found no evidence that mild asthmatics were more responsive than healthy to ozone in terms of exaggerated neutrophil recruitment or exacerbation of pre-existing allergic inflammation. Further work is needed to assess the possibility of a difference in time kinetics between healthy and asthmatic subjects in their response to ozone.
Department of Respiratory Medicine and Allergy, University Hospital, Umeå, Sweden.
N
N
Stenfors
J
J
Pourazar
A
A
Blomberg
M T
MT
Krishna
I
I
Mudway
R
R
Helleday
F J
FJ
Kelly
A J
AJ
Frew
T
T
Sandström
D000328:Adult; D000393:Air Pollutants / Q000009:adverse effects*; D001249:Asthma / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology; D001706:Biopsy; D001980:Bronchi / Q000187:drug effects / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D005260:Female; D005541:Forced Expiratory Volume / Q000187:drug effects; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020556:Neutrophil Infiltration / Q000187:drug effects*; D013464:Sulfuric Acids / Q000009:adverse effects*; D014797:Vital Capacity / Q000187:drug effects
D000393:Air Pollutants; D018836:Inflammation Mediators; D013464:Sulfuric Acids; C048813:potassium peroxymonosulfuric acid