[Expression of mRNA for proteins involved in retinol metabolism in cultured bovine iris pigment epithelial cells in vitro].
OBJECTIVE
To assess the retinol metabolism of cultured bovine iris pigment epithelial (IPE) cells.


METHODS
IPE cells were isolated from neonatal bovines with Hu's method, and were cultured. The total RNA of IPE cells was extracted by Trizol one step method. The specific primers for retinol-binding protein (RBP) and cellular retinaldehyde-binding protein (CRALBP) were designed according to their sequence from Genbank. The mRNA expression of these proteins in the IPE cells was analyzed by reverse transcription polymerase-chain reaction (RT-PCR).


RESULTS
The extracted RNA from IPE cells of the second passage was ideal and had no degradation. RT-PCR analysis showed that RBP mRNA and CRALBP mRNA were expressed in cultured IPE cells in vitro.


CONCLUSIONS
Similar to RPE cells, cultured IPE cells may have potential of transporting and metabolizing retinol.
Capital University of Medical Sciences, Beijing Tong Ren Ophthalmic Center, Beijing 100730, China. yibinlee@hotmail.com
Yibin
Y
Li
Baochen
B
Sun
D000818:Animals; D000831:Animals, Newborn; D002352:Carrier Proteins / Q000235:genetics*; D002417:Cattle; D002478:Cells, Cultured; D015870:Gene Expression; D007498:Iris / Q000166:cytology / Q000378:metabolism*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C020932:11-cis-retinal-binding protein; D002352:Carrier Proteins; D012333:RNA, Messenger; D012177:Retinol-Binding Proteins