Patterning of the vertebrate ventral spinal cord.
We review investigations that have lead to a model of how the ventral spinal cord of higher vertebrate embryos is patterned during development. Central to this model is the secreted morphogen protein, Sonic hedgehog. There is now considerable evidence that this molecule acts in a concentration-dependent manner to direct the development of the spinal cord. Recent studies have suggested that two classes of homeodomain proteins are induced by threshold concentrations of Sonic hedgehog. Reciprocal inhibition between the two classes acts to convert the continuous gradient of Sonic hedgehog into defined domains of transcription factor expression. However, a number of aspects of ventral spinal cord patterning remain to be elucidated. Some issues currently under investigation involve temporal aspects of Shh-signalling, the role of other signals in ventral patterning and the characterisation of ventral interneurons. In this review, we discuss the current state of knowledge of these issues and present some preliminary studies aimed at furthering understanding of these processes in spinal cord patterning.
Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane, Australia.
Alisa
A
Poh
Asanka
A
Karunaratne
Gabriel
G
Kolle
Ning
N
Huang
Emma
E
Smith
Joanna
J
Starkey
Daying
D
Wen
Ian
I
Wilson
Toshiya
T
Yamada
Murray
M
Hargrave
D000818:Animals; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D008954:Models, Biological; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D017434:Protein Structure, Tertiary; D015398:Signal Transduction; D013116:Spinal Cord / Q000196:embryology* / Q000502:physiology*; D013997:Time Factors; D015534:Trans-Activators / Q000378:metabolism* / Q000502:physiology*; D014158:Transcription, Genetic
D053823:Hedgehog Proteins; D015534:Trans-Activators