The effect of a reversible period of adverse intrauterine conditions during late gestation on fetal and placental weight and placentome distribution in sheep.
Adverse intrauterine conditions occurring during early to mid-gestation or throughout the whole of gestation influence placental weight and the distribution of placentome types in sheep. However, no study to date has investigated the effect of a reversible period of adverse intrauterine conditions during late gestation upon fetal and placental weight and placentome distribution in sheep. Twenty-two sheep fetuses were chronically instrumented with an inflatable cord occluder, amniotic and vascular catheters and with a Transonic flow probe around an umbilical artery. At 125 days (term isca.145 days) the occluder was inflated to reduce umbilical blood flow by ca.30 per cent for 3d in 12 fetuses (umbilical cord compressed, UCC). The occluder was then deflated and umbilical blood flow allowed to return to baseline. The remaining 10 fetuses acted as sham-operated controls in which the occluder remained deflated at all times. At 135-137dGA ewes were humanely killed and tissues collected, weighed and placentomes classified. A reduction in umbilical blood flow by approximately 30 per cent from baseline for 3 days in UCC fetuses led to mild fetal asphyxia throughout the period of cord-compression. After deflation of the occluder cuff, umbilical blood flow returned to a level that was significantly greater than that measured during baseline. Umbilical cord compression had no effect on fetal body weight but significantly increased fetal adrenal weight relative to body weight. While the total number of placentomes was not altered by cord-compression, total placentome weight and the total weight of C/D-type placentomes were both reduced in UCC relative to control placentae. In addition, the mean weight of placentomes, and of C/D-type placentomes specifically, was significantly lower in UCC relative to control placentae. When expressed as a percentage of the total number of placentomes in the placenta, there was a significantly lower percentage of C/D-type placentomes in UCC relative to control placentae. In addition, there was a significant relationship between the total number of placentomes and the percentage C/D-type placentomes in control, but not UCC, placentae. The data suggest that a temporary, reversible period of adverse intrauterine conditions occurring late in gestation in sheep has persisting effects upon the placenta, mean placentome weight and placentome distribution.
The Physiological Laboratory, University of Cambridge, Downing Street, Cambridge CB2 3EG, UK. dsg22@cam.ac.uk
D S
DS
Gardner
J W
JW
Ward
D A
DA
Giussani
A L
AL
Fowden
D000311:Adrenal Glands / Q000196:embryology / Q000473:pathology; D000818:Animals; D003251:Constriction, Pathologic; D004195:Disease Models, Animal; D005260:Female; D020567:Fetal Weight / Q000502:physiology*; D005865:Gestational Age; D009929:Organ Size / Q000502:physiology; D010929:Placentation; D011247:Pregnancy; D011248:Pregnancy Complications / Q000662:veterinary*; D011312:Pressure / Q000009:adverse effects; D012039:Regional Blood Flow / Q000502:physiology; D015427:Reperfusion Injury / Q000209:etiology / Q000503:physiopathology / Q000662:veterinary*; D014470:Umbilical Cord / Q000098:blood supply