Potato gene Y-1 is an N gene homolog that confers cell death upon infection with potato virus Y.
ADG2 is a DNA sequence mapped to a resistance (R) gene-rich region at the distal end of chromosome XI in potato (Solanum tuberosum subsp. andigena). The gene, in which ADG2 represents the predicted nucleotide-binding domain (NBS), was cloned and characterized. The coding region of the gene (designated as Y-1) is 6,187 bp long and structurally similar to gene N that confers hypersensitive resistance to Tobacco mosaic virus in Nicotiana spp. Both belong to the TIR-NBS-LRR class of genes and show 57% identity at the amino acid sequence level. The introns of Y-1 were spliced as predicted from the sequence. Y-1 cosegregated with Ry(adg), a gene for extreme resistance to Potato virus Y (PVY) on chromosome XI, as tested in a potato-mapping population and with independent potato cultivars. Leaves of the transgenic potato plants expressing Y-1 under the control of Cauliflower mosaic virus 35S promoter developed necrotic lesions upon infection with PVY, but no significant resistance was observed, and plants were systemically infected with PVY.
Vidal
Sabina
S
Department of Plant Biology, Swedish University of Agricultural Sciences, Uppsala.
Cabrera
Héctor
H
Andersson
Robert A
RA
Fredriksson
Anna
A
Valkonen
Jari P T
JP
D000595:Amino Acid Sequence; D001483:Base Sequence; D016923:Cell Death; D018076:DNA, Complementary; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified; D017800:Potyvirus / Q000472:pathogenicity*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D011198:Solanum tuberosum / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins