Measurement of the repeat period of myelin sheath using ultrathin frozen sections.
The myelin sheath of peripheral nerves was observed by transmission electron microscopy (TEM) using plastic-embedded sections and ultrathin frozen sections. Repeat distances of myelin sheaths were measured in high-powered electron micrographs. The ultrathin frozen sections showed a longer repeat distance than the plastic-embedded sections. The ultrathin frozen sections were thought to contain fewer artefacts, as they had not been subject to dehydration and embedding. It is known that broken myelin sheaths are often observed under conventional TEM. It is thought that these procedures cause contraction and partial destruction of the myelin sheath.
Osawa
Tokuji
T
Oral Anatomy I, Iwate Medical University School of Dentistry, Iwate Medical University, Morioka, Japan. tosawa@iwate-med.ac.jp
Ishida
Kinji
K
Onodera
Masao
M
Feng
Xin-Yan
XY
Hayashi
Shuichiro
S
Nozaka
Yohichiro
Y
D000818:Animals; D016616:Cryoultramicrotomy / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D009186:Myelin Sheath / Q000648:ultrastructure*; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000648:ultrastructure