Identification of apolipoprotein A-I as a major adsorbate on biomaterial surfaces after blood or plasma contact.
Apolipoprotein A-I is the major protein component of HDL. It is reported in this communication that apo A-I is a significant component of the protein layer adsorbed from blood or plasma to a variety of biomaterial surfaces, including hydrophobic and hvdrophilic polymers, liposomes, a heparinized surface, and a polysulfone hemodialyzer membrane. Evidence in support of this conclusion from SDS-PAGE and immunoblots of proteins eluted from the surfaces after blood or plasma contact is presented. Whether the presence of apo A-I on these surfaces results from the uptake of the free protein or HDL particles remains to be determined. It appears that apolipoprotein A-I and/or HDL deposition may be an important effect in blood-biomaterial interactions generally, and one that has been largely overlooked.
Department of Chemical Engineering, McMaster University, Hamilton, Ontario, Canada.
Rena M
RM
Cornelius
Jacques
J
Archambault
John L
JL
Brash
D000327:Adsorption; D016632:Apolipoprotein A-I / Q000097:blood*; D001672:Biocompatible Materials; D001769:Blood; D006493:Heparin; D006801:Humans; D066298:In Vitro Techniques; D007688:Kidneys, Artificial; D008075:Lipoproteins, HDL / Q000097:blood; D008081:Liposomes; D008422:Materials Testing; D008567:Membranes, Artificial; D010949:Plasma; D011108:Polymers; D013499:Surface Properties
D016632:Apolipoprotein A-I; D001672:Biocompatible Materials; D008075:Lipoproteins, HDL; D008081:Liposomes; D008567:Membranes, Artificial; D011108:Polymers; D006493:Heparin