Yellow lupine gene encoding stearoyl-ACP desaturase--organization, expression and potential application.
A gene for the delta9 desaturase specific to stearoyl-ACP (acyl carrier protein) was identified from yellow lupine (Lupinus luteus) cDNA and genomic libraries through the differential display method. The desaturase transcript appears in plants infected with Bradyrhizobium sp. (Lupinus) as revealed by Northern hybridization, RT-PCR and expression of beta-glucuronidase under the desaturase promoter. A small amount of desaturase transcript was also detected in uninfected plants, which suggests that the gene does not belong to the strict nodule-specific sequences. The desaturase provides unsaturated fatty acids for additional cell membrane synthesis. During nodule and symbiosome development a peribacteroid membrane is formed and the requirement for membrane surface increases, thus the level of desaturase expression is also higher. Transgenic plants of Nicotiana tabacum with overexpression of the full-length lupine stearoyl-ACP desaturase sequence were obtained. They revealed higher content of unsaturated fatty acids (especially oleic acid) in comparison with control plants.
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.
Zaneta
Z
Zaborowska
Michał
M
Starzycki
Iwona
I
Femiak
Michał
M
Swiderski
Andrzej B
AB
Legocki
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary; D005227:Fatty Acids / Q000378:metabolism; D015698:Genomic Library; D031297:Lupinus / Q000235:genetics* / Q000378:metabolism; D029902:Medicago / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; D005227:Fatty Acids; D006899:Mixed Function Oxygenases; C018747:acyl-(acyl-carrier-protein)desaturase