CCR4 in human allergen-induced late responses in the skin and lung.
We studied the regulation of CCR4 expression in peripheral blood and in human models of cutaneous and pulmonary allergen challenge. CCR4 expression was detectable on freshly isolated CD4+ lymphocytes and in CD4+ and CD8+ T cell lines derived from blood of atopic donors. Numbers of CCR4+ cells were up-regulated in T cell lines expanded in the presence of IL-4. CCR4 mRNA was absent at baseline in normal subjects in lung and skin, but present at baseline in the lung of some atopics. Baseline expression of CCR4 mRNA and protein was higher in lung vs. skin, but allergen-induced increases in CCR4 mRNA+ cells were observed in both organs. CCR4 protein+ cells were present at higher levels after allergen challenge in atopics compared to normal subjects. CCR4 may be important in the recruitment of T lymphocytes at sites of allergic inflammation, in a non-organ-specific manner.
Upper Respiratory Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College of Science, Technology and Medicine, London, GB.
Kayhan T
KT
Nouri-Aria
Duncan
D
Wilson
James N
JN
Francis
Louise A
LA
Jopling
Mikila R
MR
Jacobson
Martin R
MR
Hodge
David P
DP
Andrew
Stephen J
SJ
Till
Eva-Maria
EM
Varga
Timothy J
TJ
Williams
James E
JE
Pease
Clare M
CM
Lloyd
Ian
I
Sabroe
Stephen R
SR
Durham
D000328:Adult; D001249:Asthma / Q000097:blood / Q000276:immunology* / Q000473:pathology; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D054414:Chemokine CCL17; D054422:Chemokine CCL22; D019742:Chemokines, CC / Q000096:biosynthesis; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D005260:Female; D015870:Gene Expression; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000276:immunology* / Q000473:pathology; D008024:Ligands; D008168:Lung / Q000276:immunology*; D008297:Male; D054398:Receptors, CCR4; D019707:Receptors, Chemokine / Q000096:biosynthesis* / Q000235:genetics; D006255:Rhinitis, Allergic, Seasonal / Q000097:blood / Q000276:immunology* / Q000473:pathology; D012867:Skin / Q000276:immunology*
C106230:CCL17 protein, human; C515482:CCL22 protein, human; C515354:CCR4 protein, human; D017252:CD3 Complex; D054414:Chemokine CCL17; D054422:Chemokine CCL22; D019742:Chemokines, CC; D008024:Ligands; D054398:Receptors, CCR4; D019707:Receptors, Chemokine