Protective immunity induced by the anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum.
OBJECTIVE
To investigate the protective immunity induced by the anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum in mice.


METHODS
An orthogonal table L(16) (4 x 2(12)) was selected as the experimental design. Eight-week-old Kunming outbred mice (male and female) were randomly divided into 16 experimental groups and 2 control groups. Control groups were injected with SP2/0 ascites intraperitoneally. Mice from each group were infected with 100 +/- 2 cercariae of Schistosoma japonicum in the abdominal skin and were sacrificed on the thirtieth day postchallenge. Adult worms were recovered and counted by perfusion of the left ventricle-portal vein. The SP2/0 ascites injected mice were used as controls and the percentage of protection was calculated.


RESULTS
Active immunization of mice with NP30 could produce protection levels ranging from 22.36% to 50.46% depending on the different immunity protocols. The best immunization protocol was established from the results.


CONCLUSIONS
Active immunization with NP30 can induce a degree of protection to infection with Schistosoma japonicum cercariae and NP30 is a potential vaccine candidate against Schistosoma japonicum.
Department of Pathology, Nanjing Medical University, Nanjing 210029, China.
Zhenqing
Z
Feng
Zhenning
Z
Qiu
Yuhua
Y
Li
Yunqian
Y
Li
Changliang
C
Zhu
Wanfen
W
Xue
Xiaohong
X
Guan
D000704:Analysis of Variance; D000818:Animals; D021461:Animals, Outbred Strains; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology* / Q000627:therapeutic use; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use; D005260:Female; D008297:Male; D051379:Mice; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D016896:Treatment Outcome; D014611:Vaccination
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal