Identification of a novel HLA-DRB1 allele, DRB1*0108, by sequence-based DRB typing in two siblings.
We report here a novel DRB1 allele identified during sequence-based HLA-DRB typing. This allele was detected during routine HLA typing of a patient and his family prior to bone marrow transplantation. The new allele, DRB1*0108, was found in the patient and in a brother. Molecular cloning and sequencing confirmed that the new DRB1 allele is identical to DRB1*0101 at exon 2 except for a single nucleotide substitution at codon 37 (TauCC-->TauAlphaC), changing the encoded serine to tyrosine. This position of the beta1 domain lies in the floor of the antigen-binding groove and shows the highest polymorphism among DRB1 alleles.
Servei d'Immunologia, Servei d'Oncologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
M
M
Martinez-Gallo
L
L
Martinez-Lostao
M A
MA
Martinez-Carretero
I
I
Gago
P
P
Maroto
J L
JL
Rodriguez-Sanchez
O
O
de la Calle-Martin
D000483:Alleles; D001483:Base Sequence; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D035781:Siblings
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains