A role for cyclic AMP response element-binding protein (CREB) but not the highly similar ATF-2 protein in sterol regulation of the promoter for 3-hydroxy-3-methylglutaryl coenzyme A reductase.
Sterol regulatory element-binding proteins (SREBPs) activate promoters for key genes of metabolism to keep pace with the cellular demand for lipids. In each SREBP-regulated promoter, at least one ubiquitous co-regulatory factor that binds to a neighboring recognition site is also required for efficient gene induction. Some of these putative co-regulatory proteins are members of transcription factor families that all bind to the same DNA sequence elements in vitro and are often expressed in the same cells. These two observations have made it difficult to assign specific and redundant functions to the unique members of a specific gene family. We have used the chromatin immunoprecipitation (ChIP) technique coupled with a transient complementation assay in Drosophila SL2 cells to directly compare the ability of two members of the CREB/ATF family to function as co-regulatory proteins for SREBP-dependent activation of the HMG-CoA reductase promoter. Results from both of these experimental systems demonstrate that CREB is an efficient SREBP co-regulator but ATF-2 is not.
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900, USA.
Tawny T
TT
Ngo
Mary K
MK
Bennett
Andrew L
AL
Bourgeois
Julia I
JI
Toth
Timothy F
TF
Osborne
D051698:Activating Transcription Factor 2; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D016466:CHO Cells; D006224:Cricetinae; D017362:Cyclic AMP Response Element-Binding Protein / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics*; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D051780:Sterol Regulatory Element Binding Protein 1; D013261:Sterols / Q000494:pharmacology*; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
D051698:Activating Transcription Factor 2; D022762:CCAAT-Enhancer-Binding Proteins; D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D051780:Sterol Regulatory Element Binding Protein 1; D013261:Sterols; D014157:Transcription Factors; D006903:Hydroxymethylglutaryl CoA Reductases