Vegetative incompatibility in the ascomycete Rosellinia necatrix studied by fluorescence microscopy.
We describe our examination of the cytological characteristics of the vegetative incompatibility reaction in a filamentous ascomycetes, Rosellinia necatrix, by analyzing the fluorescence emitted by ethidium bromide and acridine orange stained nuclei. Hyphal anastomosis between incompatible strains, which were field and single ascospore isolates, were observed with cell death showing fused hyphae, and nuclei debris which were intensified by staining with ethidium bromide. In contrast, the nuclei in a living cell were not intensified by staining with ethidium bromide but were intensified by staining with acridine orange. A strain was found which did not form a barrier reaction, but which could be shown to undergo cell death and therefore showed a positive vegetative incompatibility reaction. We also examined the vegetative incompatibility among five single ascospore isolates and the putative parent strain from the same perithecium; all strains were incompatible. These results strongly suggest that vegetative incompatibility in R. necatrix is regulated by many loci.
Department of Bioresource Development, Hiroshima Prefectural University, Nanatsukahara, Shobara-shi, Hiroshima 727-0023, Japan.
Tadanori
T
Aimi
Yoshiki
Y
Yotsutani
Tsutomu
T
Morinaga
D000165:Acridine Orange; D001203:Ascomycota / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development; D003433:Crosses, Genetic; D003470:Culture Media; D004996:Ethidium; D005456:Fluorescent Dyes; D025301:Hyphae / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development; D008856:Microscopy, Fluorescence
D003470:Culture Media; D005456:Fluorescent Dyes; D004996:Ethidium; D000165:Acridine Orange