Distribution of vacA genotypes in Helicobacter pylori strains isolated from Brazilian adult patients with gastritis, duodenal ulcer or gastric carcinoma.
This PCR-based analysis is the first molecular epidemiological study in Brazil testing Helicobacter pylori cagA and vacA distribution in adults with gastric complaints, that includes a large number of carcinoma patients. Multiple-strain infection was identified in 11/13.4% patients. vacA s1-m1 and cagA(+) genotypes were the most common in patients with a non-mixed infection. All vacA s1 strains were s1b, so subtyping s1 strains was not useful. vacA s1b-m1 and cagA(+) strains were associated with higher prevalence of peptic ulcer and gastric carcinoma than vacA s2-m2 and cagA(-) ones. In conclusion, cagA and vacA genotyping may have clinical relevance in Brazil.
Ashour
Abdussalam A R
AA
Laboratory of Molecular Biology, Faculdade de Medicina, Universidade Federal de Minas Gerais, 30130-100, Belo Horizonte, Brazil.
Magalhães
Paula P
PP
Mendes
Edilberto N
EN
Collares
Guilherme B
GB
de Gusmão
Valquíria R
VR
Queiroz
Dulciene M M
DM
Nogueira
Ana Margarida M F
AM
Rocha
Gifone A
GA
de Oliveira
Celso A
CA
D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001938:Brazil / Q000453:epidemiology; D004381:Duodenal Ulcer / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D005756:Gastritis / Q000453:epidemiology / Q000382:microbiology*; D005838:Genotype; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000382:microbiology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori