Identification of Fusarium species by isozyme analysis.
Cellulose-acetate electrophoresis (CAE) was used to investigate isozyme polymorphisms among different isolates of Fusarium cerealis, F. culmorum, F. graminearum and F. pseudograminearum. After initial testing of 18 enzymes in three buffer systems for activity and resolution of bands, 12 proved to be appropriate for analysis of the full sample set. Comparing the different electrophoretic types (ETs), adenylate kinase (AK), NADP dependent glutamate dehydrogenase (NADP GDH), peptidase B (PEP B), peptidase D (PEP D) and phosphoglucomutase (PGM) proved to be diagnostic for at least one species examined. However, only PEP D was useful alone as a marker to distinguish the four taxa studied providing a rapid and simple CAE based diagnostic protocol.
Láday
M
M
Plant Protection Institute, Hungarian Academy of Sciences, P.O. Box 102, H-1525 Budapest, Hungary.
Szécsi
A
A
D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D005670:Fusarium / Q000145:classification* / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics; D016533:Mycological Typing Techniques
D007527:Isoenzymes