A method to assess biochemical activity of liver cells during clinical application of extracorporeal hybrid liver support.
Biochemical activity of a hybrid liver support system based on porcine liver cells was investigated in patients suffering from acute liver failure, coma stage III-IV Patient plasma was drawn systemically and after circulation through the bioreactor at four hour intervals. A method is used that takes into account the rate of plasma flow and the differences in plasma concentration systemically and after circulation through the liver support system to determine the net release or uptake of metabolites such as ammonia, urea and glucose. Urea release (mean 2.28+/-0.37 micromol/h/g cells) and ammonia uptake (mean 0.17+/-0.11 micromol/h/g cells) was seen during treatment, an active role of the system in glucose metabolism was observed. All patients were bridged successfully to liver transplantation.
Mundt
A
A
Surgery Clinic, Charité, Humboldt University, Berlin, Germany.
Puhl
G
G
Müller
A
A
Sauer
I
I
Müller
C
C
Richard
R
R
Fotopoulou
C
C
Doll
R
R
Gäbelein
G
G
Höhn
W
W
Hofbauer
R
R
Neuhaus
P
P
Gerlach
J
J
D000641:Ammonia / Q000032:analysis; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D019149:Bioreactors; D001783:Blood Flow Velocity; D005112:Extracorporeal Circulation; D005947:Glucose / Q000032:analysis; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism*; D017114:Liver Failure, Acute / Q000378:metabolism* / Q000628:therapy*; D016031:Liver Transplantation; D013552:Swine; D014508:Urea / Q000032:analysis
D000641:Ammonia; D014508:Urea; D005947:Glucose