Two-dimensional display and whole genome comparison of bacterial pathogen genomes of high G+C DNA content.
High-resolution comparison of bacterial genomes facilitates the identification of the genetic changes responsible for clinically relevant phenotypes. For this purpose we have established a method for the display and comparison of high G+C bacterial genomes in two dimensions. Here we describe the application of two-dimensional bacterial genomic display to resolve the genomes of Bordetella pertussis, Mycobacterium avium and Mycobacterium tuberculosis, and its utility in strain comparison and detection of insertion and substitution mutations.
Malloff
Chad A
CA
Department of Pathology, University of British Columbia, Canada.
Fernandez
Rachel C
RC
Dullaghan
Edith M
EM
Stokes
Richard W
RW
Lam
Wan L
WL
D001419:Bacteria / Q000235:genetics* / Q000472:pathogenicity; D001482:Base Composition; D001886:Bordetella pertussis / Q000235:genetics; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D016680:Genome, Bacterial; D009154:Mutation; D009162:Mycobacterium avium / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics; D015203:Reproducibility of Results; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
D004269:DNA, Bacterial