Expression and regulation of gp130 messenger ribonucleic acid in cultured immature rat Sertoli cells.
The expression of glycoprotein 130 (gp130) was studied in rat primary Sertoli cells by Northern blot analysis. Gp130 mRNA of 9.0 and 7.5 kb were detected in a variety of rat tissues including the testis. Gp130 mRNAs were detected in isolated, immature Sertoli cells. The levels of gp130 were significantly stimulated by the addition of interleukin-1-beta (IL-1-beta) or interleukin-6 (IL-6) but not by the addition of follicle-stimulating hormone (FSH). IL-6 activates intracellular signaling by binding with a receptor consisting of an 80-kDa ligand-binding protein, IL-6 receptor (IL-6R), and a second 130-kDa protein, gp130. As previously reported, expression of IL-6R mRNA in rat Sertoli cells was stimulated not only by IL-1-beta and IL-6 but also by FSH. In contrast, gp130 mRNA expression was not stimulated by FSH in our analysis. These data suggest that gp130 expression may be regulated by more than 1 mechanism and that production of gp130 and IL-6R, the 2 components of the IL-6 receptor system, may be regulated, at least in part, by a different pathway.
Department of Organ Therapeutics, Kobe University Graduate School of Medicine, Japan. masato@med.kobe-u.ac.jp
Masato
M
Fujisawa
Yoshihiro
Y
Okuda
Hitoshi
H
Fujioka
Sadao
S
Kamidono
D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D015152:Blotting, Northern; D002478:Cells, Cultured; D050822:Cytokine Receptor gp130; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005786:Gene Expression Regulation; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012708:Sertoli Cells / Q000737:chemistry / Q000378:metabolism*; D013737:Testis / Q000737:chemistry
D015703:Antigens, CD; C494201:Il6st protein, rat; D007375:Interleukin-1; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D050822:Cytokine Receptor gp130; D005640:Follicle Stimulating Hormone