A radiation hybrid map for the bovine Y Chromosome.
Screening a bovine Y Chromosome-specific DNA library resulted in 34 new microsatellites, six of which mapped to the pseudoautosomal region (PAR), and 28 localized to the Y-specific region. These microsatellites, together with 23 markers previously mapped to the bovine Y Chr, were scored on a 7000-rad cattle-hamster radiation hybrid (RH) panel. Retention frequency of individual markers ranged from 18.5% to 76.5% with an average of 48.4%. Markers with high retention frequency (>55%) were found to exist in multiple copies on the Y Chr. Thirteen markers were placed on the PAR RH map with the AmelY gene proximal to the pseudoautosomal boundary and 46 markers, including Sry and Tspy gene, on the Y-specific region of the RH map. The microsatellites developed and mapped in this work will be useful for comparative mapping of cattle, sheep, and goat, studying the origin, evolution, and migration of bovidae species and provide an initial platform to develop a high-resolution map of the Y Chr and positional cloning of Y-specific genes.
Liu
Wan-Sheng
WS
Department of Animal Science, College of Agricultural, Food, and Environmental Sciences, University of Minnesota, 305 Haecker Hall, 1364 Eckles Avenue, St. Paul 55108, USA.
Mariani
Paola
P
Beattie
Craig W
CW
Alexander
Leeson J
LJ
Ponce De León
F Abel
FA
D000818:Animals; D002417:Cattle / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D008126:Lod Score; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D021101:Radiation Hybrid Mapping; D016324:Sequence Tagged Sites; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D014998:Y Chromosome
D004268:DNA-Binding Proteins; D005819:Genetic Markers; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors