Characterization of dendrimer properties by capillary electrophoresis and their use as pseudostationary phases.
The general properties of dendrimers and in particular their electrolytic characteristics that are relevant in electrokinetic separations, are described. In order to confirm theoretical considerations on commercial dendrimer charge and hydrodynamic radius, several capillary zone electrophoresis (CZE) experiments were performed. Electrophoretic mobilities measured at different pH values indicated a sensible increase of dendrimer hydrodynamic radius at pH values lower than 2.5. This was probably due to the Coulombic repulsion of charged amine groups of the inner dendrimer shells. The principal reasons that should address the use of dendrimers as pseudostationary phases in micellar electrokinetic chromatography (MEKC) are discussed. Moreover, a survey of different separations performed utilizing dendrimers in MEKC as well as of several future plausible uses of various classes of dendrimers is presented.
Castagnola
Massimo
M
Istituto di Biochimica e Biochimica Clinica, Facoltà di Medicina e Chirurgia A. Gemelli, Università Cattolica, Roma, Italy. castagnola@uniserv.ccr.rm.cnr.it
Zuppi
Cecilia
C
Rossetti
Diana V
DV
Vincenzoni
Federica
F
Lupi
Alessandro
A
Vitali
Alberto
A
Meucci
Elisabetta
E
Messana
Irene
I
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D050091:Dendrimers; D019075:Electrophoresis, Capillary / Q000379:methods*; D011073:Polyamines
D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines