Generation of a permanent cell line that supports efficient growth of Marek's disease virus (MDV) by constitutive expression of MDV glycoprotein E.
A recombinant cell line (SOgE) was established, which was derived from the permanent quail muscle cell line QM7 and constitutively expressed the glycoprotein E (gE) gene of Marek's disease virus serotype 1 (MDV-1). The SOgE cell line supported growth of virulent (RB-1B) and vaccine (CVI988, 584Ap80C) MDV-1 strains at a level comparable with that of primary chicken embryo cells (CEC). The SOgE cell line was used to produce a vaccine against Marek's disease. Chickens were immunized at 1 day old with 10(3) p.f.u. CVI988 produced on either CEC or SOgE cells. Challenge infection was performed at day 12 with hypervirulent Italian MDV-1 strain EU1. Whereas 7/7 or 6/6 animals, respectively, immunized with SOgE or QM7 cells alone developed Marek's disease, only 1/8 animals from both CVI988-immunized groups exhibited signs of disease, suggesting that SOgE cells are a valuable permanent cell culture system for MDV-1 vaccine production.
Institute of Molecular Biology1 and Infectology2, Friedrich-Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, D-17498 Insel Riems, Germany.
Daniel
D
Schumacher
Institute of Molecular Biology1 and Infectology2, Friedrich-Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, D-17498 Insel Riems, Germany.
B Karsten
BK
Tischer
Institute of Molecular Biology1 and Infectology2, Friedrich-Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, D-17498 Insel Riems, Germany.
Jens-Peter
JP
Teifke
Institute of Molecular Biology1 and Infectology2, Friedrich-Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, D-17498 Insel Riems, Germany.
Kerstin
K
Wink
Institute of Molecular Biology1 and Infectology2, Friedrich-Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, D-17498 Insel Riems, Germany.
Nikolaus
N
Osterrieder
D000818:Animals; D002645:Chickens; D025922:Mardivirus / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity; D008380:Marek Disease / Q000517:prevention & control* / Q000821:virology; D009132:Muscles / Q000166:cytology; D011201:Poultry Diseases / Q000517:prevention & control / Q000821:virology; D011784:Quail; D014407:Tumor Cells, Cultured; D014611:Vaccination; D014759:Viral Envelope Proteins / Q000096:biosynthesis*; D014765:Viral Vaccines; D014773:Virology / Q000379:methods; D014774:Virulence; D014776:Virus Cultivation; D014779:Virus Replication
D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C106928:glycoprotein E, Marek's disease virus