A quick assay for Na+-K+-AtPase specific activity.
The method describes a simultaneous determination of inorganic phosphate (Pi) and protein content from a reaction mixture used for assay of adult rat cerebrocortical synaptosomal membrane Na+-K+-ATPase specific activity. The present method is more convenient, accurate and quicker compared to the existing methods for the determination of Na+-K+-ATPase activity. It also eliminates the possible errors in protein estimation by other classical methods in brain, which have a high lipid content.
Sarkar
Pradip K
PK
Department of Biology, Rutgers University, Camden, N.J. 08033, USA. pksarkar@crab.rutgers.edu
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D010710:Phosphates / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013574:Synaptosomes / Q000201:enzymology*
D010710:Phosphates; D000254:Sodium-Potassium-Exchanging ATPase