Influence of different neural systems on the secretion of sex hormones in potassium deficient mice.
The influence of different neural systems that modulate GnRH secretion by hypothalamic neurons was investigated in mice exposed to hypokalemic conditions, in which the pulsatile release of GnRH has been shown to be altered and associated with a significant decrease of plasma sex steroids. Our results demonstrate that the potentiation of the inhibitory pathways mediated by opiates and GABA may be implicated in the decrease of sex hormones secretion produced by hypokalemia since treatment with higher doses of naloxone or flumazenil are required to restore progesterone or testosterone levels in potassium deficient mice. The combination treatment of prazoxin and naloxone suggests that the inhibitory action of opiates take place through its action on noradrenergic neurons. It is also possible that the inhibition of GnRH release could be due to a decrease in the tonic stimulatory action of noradrenergic pathway implicated in the control of GnRH release. Our results also reveal that it is unlikely that the glutamatergic system may play any relevant direct role in the decrease of sex steroid secretion observed in potassium deficient mice. Finally, these results together with the normal pattern of estradiol levels found along the estrus cycle in potassium deficient mice indicate that factors different from estradiol and acting on neural systems implicated in the regulation of GnRH-secreting neurons participate in the generation of the preovulatory surge of GnRH.
Department of Pharmacology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain.
Francisco
F
Tejada
Rafael
R
Peñafiel
Leonor
L
Pinilla
Asunción
A
Cremades
D000818:Animals; D004359:Drug Therapy, Combination; D004958:Estradiol / Q000097:blood; D030762:Estrous Cycle / Q000097:blood; D005260:Female; D005442:Flumazenil / Q000494:pharmacology; D018757:GABA Modulators / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007008:Hypokalemia / Q000097:blood; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology*; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism*; D051379:Mice; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D011191:Potassium Deficiency / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*; D013739:Testosterone / Q000097:blood / Q000378:metabolism*
D018757:GABA Modulators; D009292:Narcotic Antagonists; D018377:Neurotransmitter Agents; D007987:Gonadotropin-Releasing Hormone; D009270:Naloxone; D013739:Testosterone; D005442:Flumazenil; D004958:Estradiol; D007986:Luteinizing Hormone; D009638:Norepinephrine