Novel complex sul1-type integron in Escherichia coli carrying bla(CTX-M-9).
For the present report, a novel complex class 1 integron, In60, was characterized. Part of this integron includes the bla(CTX-M-9) gene and its downstream nucleotide sequence, which shares 81% and 78% nucleotide identity with those of kluA-1 beta-lactamase and orf3 of K. ascorbata, respectively. Furthermore, a new insertion sequence, IS3000, has been found in In60. PCR analysis indicates that integron In60 is present in 33 of 34 nonclonal enterobacterial isolates carrying the putative beta-lactamase CTX-M-9.
Sabaté
Montserrat
M
Departament de Microbiologia, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma, Av. Sant Antoni M Claret 167, 08025 Barcelona, Spain.
Navarro
Ferran
F
Miró
Elisenda
E
Campoy
Susana
S
Mirelis
Beatriz
B
Barbé
Jordi
J
Prats
Guillem
G
D001483:Base Sequence; D017124:Conserved Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D004755:Enterobacteriaceae / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005656:Fungal Proteins / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D001618:beta-Lactamases / Q000235:genetics*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C090870:FZF1 protein, S cerevisiae; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C412613:CTX-M-9 protein, E coli; D001618:beta-Lactamases