Plasmid DNA encoding the respiratory syncytial virus G protein protects against RSV-induced airway hyperresponsiveness.
Respiratory syncytial virus (RSV) is an important cause of childhood respiratory disease as well as exacerbations of asthma. Although previous studies have demonstrated that a DNA vaccine encoding the RSV G protein can inhibit RSV replication in mouse models of RSV infection, studies have not been performed to determine whether a DNA vaccine encoding the RSV G protein can protect against RSV induced mucus expression and airway hyperresponsiveness which was the focus of this study. The DNA-G vaccine we constructed significantly inhibited RSV viral replication, mucus expression, and importantly was associated with inhibition of RSV induced airway responsiveness.
Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0635, USA.
Marina
M
Miller
Jae Youn
JY
Cho
Kwang Je
KJ
Baek
Diego
D
Castaneda
Jyothi
J
Nayar
Monica
M
Rodriguez
Mark
M
Roman
Eyal
E
Raz
David H
DH
Broide
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D018793:Interleukin-13 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009093:Mucus / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000517:prevention & control; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D019444:Vaccines, DNA / Q000235:genetics / Q000494:pharmacology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000494:pharmacology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D018793:Interleukin-13; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines