First in vitro norlignan formation with Asparagus officinalis enzyme preparation.
We report for the first time that an enzyme preparation from fungal-elicited Asparagus officinalis cultured cells catalyses the formation of a norlignan, (Z)-hinokiresinol, from two non-identical phenylpropanoid monomers, 4-coumaryl alcohol and 4-coumaroyl CoA, and from a dimer, 4-coumaryl 4-coumarate, without any additional cofactors.
Suzuki
Shiro
S
Wood Research Institute, Kyoto University, Uji, Kyoto 611-0011, Japan.
Nakatsubo
Tomoyuki
T
Umezawa
Toshiaki
T
Shimada
Mikio
M
D000214:Acyl Coenzyme A / Q000378:metabolism*; D027761:Asparagus Plant / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D002478:Cells, Cultured; D017705:Lignans / Q000096:biosynthesis*; D013058:Mass Spectrometry
D000214:Acyl Coenzyme A; D017705:Lignans; C058644:4-coumaroyl-coenzyme A