tej defines a role for poly(ADP-ribosyl)ation in establishing period length of the arabidopsis circadian oscillator.
In a genetic screen for altered circadian period length in Arabidopsis, we isolated a mutant with a long free-running period. The tej mutation acts independently of light quality and quantity. It affects clock-controlled transcription of genes in Arabidopsis and alters the timing of the photoperiod-dependent transition from vegetative growth to flowering. Map-based cloning of TEJ identified a poly(ADP-ribose) glycohydrolase (PARG). An inhibitor of poly(ADP-ribosyl)ation rescued the period phenotype of tej mutant and shortened the period length of wild-type plants. Posttranslational poly(ADP-ribosyl)ation of an oscillator component may contribute to setting the period length of the Arabidopsis central oscillator.
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Satchidananda
S
Panda
Guy G
GG
Poirier
Steve A
SA
Kay
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D001549:Benzamides / Q000494:pharmacology; D001683:Biological Clocks / Q000235:genetics*; D002940:Circadian Rhythm / Q000235:genetics*; D003001:Cloning, Molecular; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010507:Periodicity; D010641:Phenotype; D010775:Photic Stimulation; D017440:Photoperiod; D011064:Poly Adenosine Diphosphate Ribose / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D029681:Arabidopsis Proteins; D001549:Benzamides; D004791:Enzyme Inhibitors; D011064:Poly Adenosine Diphosphate Ribose; C025160:3-aminobenzamide; D006026:Glycoside Hydrolases; C022407:poly ADP-ribose glycohydrolase