Genetics of sulphate assimilation in Schizosaccharomyces pombe (a short review).
Sulphur plays an important role in yeasts, especially in the biosynthesis of methionine and cysteine. The inorganic sulphur source, sulphate, is taken up by the cells via the sulphate-permease(s). After its transport, it is activated and subsequently reduced to sulphide or serves as a donor for sulphurylation reactions. Selenate anion (SeO4(2-)), which has the same metabolic pathway as sulphate, is toxic for the cells of Schizosaccharomyces pombe. We isolated selenate resistant mutants which cannot utilize sulphate, therefore they need organic sulphur source for growth. One of the selenate resistant mutants was successively transformed with S. pombe genomic libraries and the gene complementing the selenate resistance was identified as that of coding for the ATP-sulphurylase enzyme.
Simonics
T
T
Szent István University, Faculty of Food Science, Department of Microbiology and Biotechnology, Somlói út 14-16, H-1118 Budapest, Hungary.
Bánszky
Luca
L
Maráz
Anna
A
D003470:Culture Media; D025141:Drug Resistance, Fungal / Q000235:genetics; D009154:Mutation; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D064586:Selenic Acid; D018036:Selenium Compounds / Q000494:pharmacology; D013430:Sulfate Adenylyltransferase / Q000235:genetics; D013431:Sulfates / Q000378:metabolism*
D003470:Culture Media; D018036:Selenium Compounds; D013431:Sulfates; D013430:Sulfate Adenylyltransferase; D064586:Selenic Acid