Ca(II) -gamma-thionin complex: interaction studies by differential pulse voltammetry and MALDI-TOF/MS.
In this work we investigated the formation of a complex between gamma-thionin SI alpha1 and Ca(2+) using differential pulse voltammetry and MALDI TOF/MS. A stoichiometry rate of one calcium ion per one SI alpha1 molecule was obtained. K(d) values of 1.31 x 10(-9) mol L(-1), 2.63 x 10(-8) mol L(-1) and 2.71 x 10(-8) mol L(-1) were determined for the Ca(2+)- SI alpha1 complex by three different methodologies. These findings contribute to a better understanding of the SI alpha1 inhibition mechanism towards insect and mammalian alpha-amylases.
de Castro
Clarissa Silva Pires
CS
Laboratório de Química Analítica Ambiental, Instituto de Química, Universidade de Brasília, P.O. Box 04394, 70.9190970, Brasília-DF, Brazil.
DeSouza
Rodrigues
R
Bloch
Carlos
C
D023181:Antimicrobial Cationic Peptides; D029681:Arabidopsis Proteins; D002118:Calcium / Q000378:metabolism*; D004563:Electrochemistry; D008628:Mercury / Q000737:chemistry; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D023181:Antimicrobial Cationic Peptides; D029681:Arabidopsis Proteins; D010940:Plant Proteins; C097482:THI2.1 protein, Arabidopsis; D008628:Mercury; D002118:Calcium