Human mitochondrial transcription factor A binds preferentially to oxidatively damaged DNA.
Mitochondrial transcription factor A (mtTFA) is necessary for both transcription and maintenance of mtDNA, and is also one of the high mobility group (HMG) proteins that preferentially binds to cisplatin-damaged DNA. In this study we confirmed the preferential binding of mtTFA to cisplatin-damaged DNA, and also discovered that mtTFA binds to oxidatively damaged DNA. The affinity for oxidatively damaged DNA of mtTFA is higher for A/8-oxo-dG and C/8-oxo-dG than for G/8-oxo-dG and T/8-oxo-dG. Our findings suggest that mtTFA plays an important role in the recognition of oxidative DNA damage.
Department of Molecular Biology, School of Medicine, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu, Fukuoka, Japan.
Yoichiro
Y
Yoshida
Hiroto
H
Izumi
Tomoko
T
Ise
Hidetaka
H
Uramoto
Takayuki
T
Torigoe
Hiroshi
H
Ishiguchi
Tadashi
T
Murakami
Mizuho
M
Tanabe
Yoshifumi
Y
Nakayama
Hideaki
H
Itoh
Hiroshi
H
Kasai
Kimitoshi
K
Kohno
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002945:Cisplatin / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; D010100:Oxygen / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015534:Trans-Activators; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D029867:Xenopus Proteins
D003432:Cross-Linking Reagents; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins; C499631:TFAM protein, human; D015534:Trans-Activators; D014157:Transcription Factors; C097078:XL-MTTFA protein, Xenopus; D029867:Xenopus Proteins; C422562:mitochondrial transcription factor A; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D005982:Glutathione Transferase; D003849:Deoxyguanosine; D002945:Cisplatin; D010100:Oxygen