[Regulating role of various amino acids in development of apoptosis in organotypic culture of the nervous and lymphoid tissue].
The effect of aminoacids L-arginin, L-lysine, L-asparagin was investigated in organotypic tissue culture of brain cortex and spleen of 1-day old rats. The aminoacids in concentrations 0.05 and 0.1 ng/ml are active, inducing a less intensive growth zone as compared to the control, excluding the effect of asparagin on lymphoid tissue. Method of fluorescent staining shows a negative correlation between growth zone size and apoptotic cell number. The nerve and lymphoid tissue express apoptosis in response to aminoacids.
I. P. Pavlov Institute of Physiology, Russian Acad. Sci., St. Petersburg, 199034, Nab. Makarova, 6, Russia.
N I
NI
Chalisova
V A
VA
Pennijainen
G
G
Haase
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis; D001120:Arginine / Q000494:pharmacology; D001216:Asparagine / Q000494:pharmacology; D001921:Brain / Q000166:cytology / Q000187:drug effects*; D046508:Culture Techniques; D008239:Lysine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000187:drug effects*
D000596:Amino Acids; D001216:Asparagine; D001120:Arginine; D008239:Lysine