Linkage analysis of a region on chromosome 2 with essential hypertension in Chinese families.
OBJECTIVE
To verify the linkage of the candidate regions identified in a previous study (markers D2S168, D2S151, D2S142 on chromosome 2) with hypertension in Chinese families.


METHODS
A genetic linkage study focused on chromosome 2 was performed on 240 Chinese families containing 856 patients with essential hypertension. A total of 1080 individuals were genotyped using 9 highly polymorphic microsatellite markers around the candidate regions on chromosome 2 with an average spacing of 5 cM. Non-parametric linkage (NPL), parametric linkage analysis and transmission-disequilibrium test (TDT) with the GENEHUNTER software were used to assess evidence for linkage.


RESULTS
Linkage of a region on chromosome 2 around D2S151 and D2S142 with hypertension was confirmed by different statistical methods (NPL, LOD score and TDT). However, the linkage of D2S168 could not be replicated in this extension study.


CONCLUSIONS
The data suggest that a region on chromosome 2 at or near the loci of D2S142 and D2S151 may harbor genes responsible for the development of essential hypertension in Chinese.
Shanghai Institute of Hypertension, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. f97075@guomai.sh.cn
Dingliang
D
Zhu
Wei
W
Huang
Houyi
H
Wang
Momiao
M
Xiong
Shaoli
S
Chu
Li
L
Jin
Guliang
G
Wang
Xin
X
He
Wentao
W
Yuan
Yuecheng
Y
Qian
Guangsheng
G
Zhao
D000483:Alleles; D002681:China; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D005192:Family Health; D005260:Female; D005787:Gene Frequency; D008040:Genetic Linkage; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D015810:Linkage Disequilibrium; D008297:Male; D018895:Microsatellite Repeats