Role of the high-affinity zinc uptake znuABC system in Salmonella enterica serovar typhimurium virulence.
The Salmonella enterica serovar Typhimurium znuABC genes encoding a high-affinity zinc uptake system and its regulatory zur gene have been cloned. Salmonella serovar Typhimurium zur and znuC knockout mutants have been constructed by marker exchange. The 50% lethal dose of the znuC mutant increased when either orally or intraperitoneally inoculated in BALB/c mice, while virulence of the zur mutant decreased only when mice were intraperitoneally challenged.
Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain.
Susana
S
Campoy
Mónica
M
Jara
Núria
N
Busquets
Ana M
AM
Pérez De Rozas
Ignacio
I
Badiola
Jordi
J
Barbé
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D027682:Cation Transport Proteins; D002460:Cell Line; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000382:microbiology; D029968:Escherichia coli Proteins; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D016296:Mutagenesis; D010641:Phenotype; D012480:Salmonella Infections / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000472:pathogenicity*; D013552:Swine; D014774:Virulence; D015032:Zinc / Q000378:metabolism*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C464647:ZnuC protein, Salmonella enterica; C114819:Zur protein, E coli; D015032:Zinc