[Influence of cytokine gene polymorphism on renal transplantation].
OBJECTIVE
To study the influence of cytokine gene polymorphism on the incidence of acute rejection in renal transplantation.


METHODS
The cytokine genotypes of TNF-alpha, TGF-beta(1), IL-10 and IL-6 in 24 normal people and 115 consecutive renal transplant recipients were determined by PCR-SSP using sequence-specific oligonucleotide primer. The cytokine genotypes were then correlated with the incidence of acute rejection in renal transplantation.


RESULTS
The frequency of TNF-alpha and IL-10 low producer genotype in the 115 kidney recipients and control group was 88.7%, 80% and 87.5%, 75% respectively, and the frequency of TGF-beta(1) and IL-6 high producer genotype was 79.1%, 100% and 66.7%, 100% respectively. The incidence of acute rejection was higher in the recipients with TNF-(high producer or IL-10 intermediate high producer genotype than in the recipients with low producer genotype [53.8%, 43.5% vs 18.6% (chi(2) = 8.17), 17.4% (chi(2) = 7.16), P < 0.01, respectively].


CONCLUSIONS
This study demonstrated that TNF-alpha and IL-10 gene polymorphism has significant influence on the incidence of acute rejection in renal transplantation.
Department of Urology, Beijing Friendship Hospital Capital University of Medical Sciences, Beijing 100050, China.
Ye
Y
Tian
Weiran
W
Ma
Yuhai
Y
Zhang
D000293:Adolescent; D000328:Adult; D000368:Aged; D016207:Cytokines / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D006084:Graft Rejection / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics; D016030:Kidney Transplantation / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10