Respiratory syncytial virus assembly occurs in GM1-rich regions of the host-cell membrane and alters the cellular distribution of tyrosine phosphorylated caveolin-1.
We have previously shown that respiratory syncytial virus (RSV) assembly occurs within regions of the host-cell surface membrane that are enriched in the protein caveolin-1 (cav-1). In this report, we have employed immunofluorescence microscopy to further examine the RSV assembly process. Our results show that RSV matures at regions of the cell surface that, in addition to cav-1, are enriched in the lipid-raft ganglioside GM1. Furthermore, a comparison of mock-infected and RSV-infected cells by confocal microscopy revealed a significant change in the cellular distribution of phosphocaveolin-1 (pcav-1). In mock-infected cells, pcav-1 was located at regions of the cell that interact with the extracellular matrix, termed focal adhesions (FA). In contrast, RSV-infected cells showed both a decrease in the levels of pcav-1 associated with FA and the appearance of pcav-1-containing cytoplasmic vesicles, the latter being absent in mock-infected cells. These cytoplasmic vesicles were clearly visible between 9 and 18 h post-infection and coincided with the formation of RSV filaments, although we did not observe a direct association of pcav-1 with mature virus. In addition, we noted a strong colocalization between pcav-1 and growth hormone receptor binding protein-7 (Grb7), within these cytoplasmic vesicles, which was not observed in mock-infected cells. Collectively, these findings show that the RSV assembly process occurs within specialized lipid-raft structures on the host-cell plasma membrane, induces the cellular redistribution of pcav-1 and results in the formation of cytoplasmic vesicles that contain both pcav-1 and Grb7.
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK1.
Gaie
G
Brown
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK1.
Helen W McL
HWM
Rixon
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK1.
Richard J
RJ
Sugrue
D000818:Animals; D051242:Caveolin 1; D022461:Caveolins / Q000378:metabolism*; D002522:Chlorocebus aethiops; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D005677:G(M1) Ganglioside / Q000378:metabolism*; D051382:GRB7 Adaptor Protein; D006801:Humans; D021962:Membrane Microdomains / Q000378:metabolism / Q000821:virology*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism; D018113:Respiratory Syncytial Virus, Human / Q000254:growth & development* / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism; D014709:Vero Cells; D019065:Virus Assembly
C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; C495955:GRB7 protein, human; D011506:Proteins; D051382:GRB7 Adaptor Protein; D005677:G(M1) Ganglioside; D014443:Tyrosine