Effects of indomethacin on cerebral blood flow at rest and during hypercapnia: an arterial spin tagging study in humans.
OBJECTIVE
To investigate using an arterial spin tagging (AST) approach the effect of indomethacin on the cerebral blood flow (CBF) response to hypercapnia.


METHODS
Subjects inhaled a gas mixture containing 6% CO(2) for two 5-minute periods, which were separated by a 10-minute interval, in which subjects inhaled room air. In six subjects, indomethacin (i.v., 0.2 mg/kg) was infused in the normocapnic interval between the two hypercapnic periods.


RESULTS
Indomethacin reduced normocapnic gray matter CBF by 36 +/- 5% and reduced the CBF increase during hypercapnia from 43 +/- 9% to 16 +/- 5% in gray matter (P < 0.001) and from 48 +/- 11% to 35 +/- 9% in white matter (P < 0.025).


CONCLUSIONS
The results demonstrate that an AST approach can measure the effects of indomethacin on global CBF increases during hypercapnia and suggest that an AST approach could be used to investigate pharmacological effects on focal CBF increases during functional activation.
St  Lawrence
Keith S
KS
Laboratory of Diagnostic Radiology Research, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA. Klawrence@mail.cc.nih.gov
Ye
Frank Q
FQ
Lewis
Bobbi K
BK
Weinberger
Daniel R
DR
Frank
Joseph A
JA
McLaughlin
Alan C
AC
D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000473:pathology* / Q000503:physiopathology*; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D005260:Female; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D006801:Humans; D006935:Hypercapnia / Q000473:pathology* / Q000503:physiopathology*; D007213:Indomethacin / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging; D008297:Male; D012039:Regional Blood Flow / Q000187:drug effects; D012146:Rest / Q000502:physiology*; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007213:Indomethacin