The allergenicity of soybean-based products is modified by food technologies.
BACKGROUND
Numerous products based on soybean are available and various food technologies are applied for their production. The allergenicity of natural soybean may be modified by these treatments.


OBJECTIVE
To compare the allergenicity of native soybean proteins with those of soy milk and texturized protein products. To show additional allergens.


METHODS
Three commercial products and two infant formulas were studied: Soybean flour, soy milk, texturized soy proteins, two infant formulas; the first containing total proteins and the second containing a soy protein hydrolysate. Sera from 9 patients allergic to soy protein were tested by immunoblotting (IB). IB inhibition was achieved by incubating sera with protein extract from soybean flour.


RESULTS
The SDS-PAGE profile of soybean flour protein and soy milk showed a difference in the proportions of the various protein fractions, with a higher concentration of 37-kD protein in flour and 33-kD protein in milk. Infant formula 1 contained proteins with a molecular weight below 28 kD. The texturized extract contained high proportions of 31- to 34- and 38-kD proteins. Immunoblotting revealed a lack of allergenicity in infant formula. Sera recognizing the 38- and 50-kD proteins in texturized soy protein also recognized the 37- and 49-kD proteins in soybean flour and in soy milk, suggesting a protein glycation by texturization processes. The 30- to 34-kD band in texturized proteins was devoid of any allergenicity. This study seems to indicate that the 30-kD allergen (Gly m Bd 30) disappears during the production of texturized soy protein.


CONCLUSIONS
All technologies applied to soybean-based products induce striking variation in the protein profile and allergenicity. Texturized protein could lack the major allergen Gly m Bd 30. Further studies or texturization might generate modified technologies in order to create hypoallergenic texturized proteins.
Laboratoire de Biochimie, Hôpital Central, Nancy, France. p.franck@chu-nancy.fr
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P
Franck
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DA
Moneret Vautrin
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B
Dousset
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G
Kanny
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P
Nabet
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Guénard-Bilbaut
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Parisot
D000293:Adolescent; D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004044:Dietary Proteins / Q000032:analysis / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005511:Food Handling / Q000379:methods*; D005512:Food Hypersensitivity / Q000276:immunology*; D005524:Food Technology; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008875:Middle Aged; D011852:Radioallergosorbent Test; D012882:Skin Tests; D030262:Soybean Proteins / Q000032:analysis / Q000276:immunology; D013025:Glycine max / Q000737:chemistry / Q000276:immunology*
D000485:Allergens; D004044:Dietary Proteins; D030262:Soybean Proteins; D007073:Immunoglobulin E