Interfacial micromorphological differences in hybrid layer formation between water- and solvent-based dentin bonding systems.
BACKGROUND
Many dentin bonding systems of different compositions, and in particular containing different solvents, have been introduced to the market. Their effect on the quality of the interface requires clarification by means of comparative trials.


OBJECTIVE
This study investigated micromorphological differences in hybrid layer formation with a variety of commercially available water- or solvent-based dentin bonding products and their recommended compomers.


METHODS
Five bonding systems were used on groups of 10 teeth each as follows: group I, acetone-based system used with 36% phosphoric acid; group II, a different acetone-based system containing nano-sized particles for filler loading and used with a non-rinsing conditioner containing maleic acid; group III, the acetone-based system of group II used with 36% phosphoric acid (the only difference in the treatment for groups II and III was the acid etching system); group IV, a mixed-solvent-based system (water/ethanol) used with 37% phosphoric acid; and group V, a water-based system used with 37% phosphoric acid. Each bonding system was covered with the recommended compomer. Class I occlusal preparations were made in extracted teeth and restored with one of the above systems. Five specimens of each group were studied with optical microscopy after staining. Scanning electron microscopy was used to examine the interface of the bonding system/dentin of the other 5 teeth in each group. The optical microscopy measurements were made with a 10 x 10 reticle. A micron mark with scale was used for the scanning electron microscope. All measurements were made in microm. The following criteria were used to define a good interface: absence of voids between the different parts of the interface, uniformity of the hybrid layer, good opening of the tubuli orifices, and tag adherence to the tubuli walls.


RESULTS
Morphological differences were found at the interface depending on dentin treatment and adhesive composition. The acetone-containing systems were associated with a continuous, gap-free hybrid layer that was linked intimately with the dentin. The tags adhered well to the tubuli walls and were often joined by side branches. In the water-based solvent systems, a lack of contact was visible between the resin tags and the tubuli walls, with some incompletely filled tubuli and some gaps in the hybrid layer. The 2 observational methods used, optical and scanning electron microscopy, proved to be complementary.


CONCLUSIONS
Within the limitations of this study, use of the acetone-based systems after phosphoric acid etching resulted in a continuous, thick hybrid layer with reverse-cone-shaped tags in close contact with the tubuli walls. Use of the water-based systems resulted in a thinner hybrid layer with some incompletely sealed dentinal tubules.
Biomaterials Laboratory, Faculty of Odontology, University of Toulouse III , Université Paul Sabatier, Toulouse, France. gregoire@cict.fr
Geneviève L
GL
Gregoire
Bernadette A
BA
Akon
Arlette
A
Millas
D000096:Acetone / Q000737:chemistry; D000134:Acid Etching, Dental; D000293:Adolescent; D000328:Adult; D004396:Coloring Agents; D020770:Compomers / Q000737:chemistry*; D003737:Dental Cavity Preparation / Q000145:classification; D003793:Dental Restoration, Permanent / Q000145:classification; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D006801:Humans; D008298:Maleates / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D010756:Phosphoric Acids / Q000737:chemistry; D017640:Silicates / Q000737:chemistry; D016037:Single-Blind Method; D012997:Solvents / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
D004396:Coloring Agents; D020770:Compomers; D017220:Dentin-Bonding Agents; C088486:Dyract; D008298:Maleates; D008689:Methacrylates; D010756:Phosphoric Acids; D017640:Silicates; D012997:Solvents; D014867:Water; D000096:Acetone; D000431:Ethanol; C030272:maleic acid