A review of aerobic and psychrotrophic plate count procedures for fresh meat and poultry products.
This is a review of reports that employed aerobic plate counts on fresh meat and poultry products since 1985; it lists synopses of 100 applications. A total of 15 different plating media were used, with 48 (48%) being either plate count agar (PCA) or tryptone glucose yeast extract agar. The temperature-time relations ranged from a low temperature of 20 degrees C for 120 h to 37 degrees C for 24 h. Some 29 different temperature-time combinations were used among the total of 109, with 21 (19.3%) being 35 degrees C/48 h, followed by 12 (11.0%) at 32 degrees C/48 h, 11 (10.1%) at 25 degrees C/48 h, and 9 (8.3%) at 25 degrees C/72 h. Fifty-four (49.5%) plate count applications employed incubation temperatures of 30 degrees C and below. From the 26 reports that employed psychrotrophic counts, 16 (61.5%) used PCA; 18 different temperature-time combinations were used, with 7 degrees C/10 d employed by only four. Twenty-one (80.8%) employed an incubation temperature at or <10 degrees C, and five employed an incubation temperature >10 degrees C. There is a serious need for some consensus on methodologies for aerobic and psychrotrophic counts on fresh meat and poultry products.
Department of Biological Sciences,University of Nevada, Las Vegas, 89154-4004, USA. jayj@univ.edu
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JM
Jay
D000362:Agar / Q000737:chemistry; D000818:Animals; D001420:Bacteria, Aerobic / Q000254:growth & development* / Q000302:isolation & purification; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D005516:Food Microbiology; D008461:Meat Products / Q000382:microbiology*; D011202:Poultry Products / Q000382:microbiology*; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D000362:Agar