Crystallization and preliminary X-ray analysis of the matrix protein of Borna disease virus.
The matrix protein M of Borna disease virus (BDV) is associated with the inner viral membrane and is thought to be a mediator between the nucleocapsid and the lipid-containing envelope in stabilizing the virus shape. The full-length BDV-M gene encoding a 16 kDa protein was expressed in Escherichia coli. M was purified to homogeneity and crystallized by the sitting-drop vapour-diffusion method. The crystals of M belong to the space group I432, with unit-cell parameters a = b = c = 144.6 A, and diffract to 3.1 A.
Institut für Virologie, Robert-Koch-Strasse 17, 35037 Marburg, Germany.
Ina
I
Kraus
Hanno
H
Scheffczik
Markus
M
Eickmann
Simone
S
Kiermayer
Milton T
MT
Stubbs
Wolfgang
W
Garten
D001483:Base Sequence; D001891:Borna disease virus / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D004279:DNA, Viral / Q000235:genetics; D015394:Molecular Structure; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D014763:Viral Matrix Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D004279:DNA, Viral; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins