Direct surface analysis of fungal species by matrix-assisted laser desorption/ionization mass spectrometry.
In this study various methods of sample preparation and matrices were investigated to determine optimum collection and analysis criteria for fungal analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Intact spores and/or hyphae of Aspergillus niger, Rhizopus oryzae, Trichoderma reesei and Phanerochaete chrysosporium were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The fungal samples were applied to the MALDI sample target as untreated, sonicated, or acid/heat treated samples, or blotted directly from the fungal culture with double-stick tape. Ferulic acid or sinapinic acid matrix solution was layered over the dried samples and analyzed by MALDI-MS. Statistical analysis showed that simply using double-stick tape to collect and transfer to a MALDI sample plate typically worked as well as the other preparation methods, and required the least sample handling.
Pacific Northwest National Laboratory, Richland, Washington 99352, USA. Nancy.valentine@pnl.gov
Nancy B
NB
Valentine
Jon H
JH
Wahl
Mark T
MT
Kingsley
Karen L
KL
Wahl
D001234:Aspergillus niger / Q000145:classification; D003373:Coumaric Acids; D005561:Formates; D005658:Fungi / Q000145:classification*; D006358:Hot Temperature; D025301:Hyphae / Q000145:classification; D012233:Rhizopus / Q000145:classification; D013010:Sonication; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013172:Spores, Fungal / Q000145:classification; D014242:Trichoderma / Q000145:classification
D003373:Coumaric Acids; D005561:Formates; C030544:formic acid; C073734:sinapinic acid; C004999:ferulic acid