Pandrug-resistant Acinetobacter baumannii causing nosocomial infections in a university hospital, Taiwan.
The rapid emergence (from 0% before 1998 to 6.5% in 2000) of pandrug-resistant Acinetobacter baumannii (PDRAB) was noted in a university hospital in Taiwan. To understand the epidemiology of these isolates, we studied 203 PDRAB isolates, taken from January 1999 to April 2000: 199 from 73 hospitalized patients treated at different clinical settings in the hospital and 4 from environmental sites in an intensive-care unit. Pulsed-field gel electrophoresis analysis and random amplified polymorphic DNA (RAPD) generated by arbitrarily primed polymerase chain reaction of these 203 isolates showed 10 closely related genotypes (10 clones). One (clone 5), belonging to pulsotype E and RAPD pattern 5, predominated (64 isolates, mostly from patients in intensive care). Increasing use of carbapenems and ciprofloxacin (selective pressure) as well as clonal dissemination might have contributed to the wide spread of PDRAB in this hospital.
Hsueh
Po-Ren
PR
National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan. hsporen@ha.mc.ntu.edu.tw
Teng
Lee-Jene
LJ
Chen
Cheng-Yi
CY
Chen
Wen-Hwei
WH
Yu
Chong-Jen
CJ
Ho
Shen-Wu
SW
Luh
Kwen-Tay
KT
D000150:Acinetobacter / Q000187:drug effects* / Q000235:genetics; D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002353:Carrier State / Q000382:microbiology; D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology*; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial; D004357:Drug Synergism; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D013624:Taiwan
D000900:Anti-Bacterial Agents