An arming yeast with the ability to entrap fluorescent 17beta-estradiol on the cell surface.
We constructed a novel surface-engineered yeast displaying the ligand-binding domain of the rat estrogen receptor (ERLBD). ERLBD, display of which on the yeast cell surface was confirmed by immunofluorescence, possessed strong binding activity to fluorescent 17beta-estradiol - an analogue of the natural ligand of the estrogen receptor - that was comparable to the activity of the native receptor. Environmental homeostasis has recently been disturbed by endocrine disruptors, which cause confusion in the hormone secretion system. It is therefore very important to identify chemical compounds with hormone-like activity and remove them from the environment. The present results demonstrate that the new arming yeast displaying ERLBD on its cell surface will be capable of screening, entrapping, and removing estradiol-like compounds from the environment.
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Yoshida, Sakyo-ku, Japan.
M
M
Yasui
S
S
Shibasaki
K
K
Kuroda
M
M
Ueda
N
N
Kawada
J
J
Nishikawa
T
T
Nishihara
A
A
Tanaka
D000818:Animals; D001665:Binding Sites; D004958:Estradiol / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D051381:Rats; D011960:Receptors, Estrogen / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D011960:Receptors, Estrogen; D011994:Recombinant Proteins; D004958:Estradiol