Tramtrack69 is required for the early repression of tailless expression.
During embryogenesis, the activated Torso receptor tyrosine kinase (TOR RTK) pathway activates tailless (tll) expression by a relief-of-repression mechanism. Various lines of evidence have suggested that multiple factors are required for this repression. We show that Tramtrack69 (TTK69) binds to two sites within tll cis-regulatory DNA, TC2 and TC5, and that TTK69 is phosphorylated by mitogen activated protein kinase. In embryos lacking maternal ttk69 activity, the expression of both endogenous tll and lacZ driven by the tll minimal regulatory region (tll-MRR) are expanded. Further, in wild-type embryos, the tll-MRR mutated in TC5 drives uniform lacZ expression before late stage 4. We conclude that TTK69 is required for early (before the end of stage 4) repression of tll transcription.
Institute of Genetics, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan, ROC.
Yueh-Jung
YJ
Chen
Chuen-Sheue
CS
Chiang
Li-Chuan
LC
Weng
Judith A
JA
Lengyel
Gwo-Jen
GJ
Liaw
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D007763:Lac Operon; D008297:Male; D009154:Mutation; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D012097:Repressor Proteins; C074090:TLL protein, Drosophila; C075878:ttk protein, Drosophila; D004247:DNA; D020794:Receptor Protein-Tyrosine Kinases; C080148:tor protein, Drosophila