Liquid chromatographic method for determination of piperine in rat plasma: application to pharmacokinetics.
Piperine, a major alkaloid of Piper longum and Piper nigrum has been reported to have several pharmacological/toxicological effects. Though a number of methods for analysis of this omnipresent food component in pepper fruits are available, its analysis in body fluids has been largely neglected. A high-performance liquid chromatography method for the analysis of piperine in rat plasma is presented in this communication. Analysis was performed using a Symmetry C(18) column (250x4.6 mm) by isocratic elution with 25 mM KH(2)PO(4) (pH 4.5)-acetonitrile (35:65) and UV detection at 340 nm. The calibration plot was linear over the range studied (2-2000 ng) with correlation coefficient of 0.9984. Limit of detection and limit of quantitation were 1 ng/ml and 3 ng/ml, respectively. Good overall recovery (85.5+/-6%) was obtained with 4 ml ethyl acetate and extraction time of 3 min. Intra- and inter-assay coefficient of variation was found to be less than 7.5%. Plasma concentration-time profile of piperine in a conscious rat implanted with jugular vein cannula was obtained using this method. The method is simple, sensitive and reproducible.
Bajad
Sunil
S
Pharmacology Division, Regional Research Laboratory, Canal Road, Jammu 180 001, India. sbajad@yahoo.com
Singla
A K
AK
Bedi
K L
KL
D000470:Alkaloids; D000818:Animals; D052117:Benzodioxoles; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010880:Piperidines / Q000097:blood* / Q000493:pharmacokinetics; D053284:Polyunsaturated Alkamides; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D000470:Alkaloids; D052117:Benzodioxoles; D010880:Piperidines; D053284:Polyunsaturated Alkamides; C008922:piperine