Inclusion of phosphatase inhibitors during Western blotting enhances signal detection with phospho-specific antibodies.
Department of Neurobiology and Behavior, McGaugh Hall, University of California at Irvine, 92697, USA.
Shiv K
SK
Sharma
Thomas J
TJ
Carew
D000818:Animals; D001048:Aplysia / Q000187:drug effects / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000276:immunology; D020935:MAP Kinase Signaling System; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D010766:Phosphorylation; D035422:Pleural Cavity; D012969:Sodium Fluoride / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology
D004791:Enzyme Inhibitors; D007074:Immunoglobulin G; D014638:Vanadates; D012969:Sodium Fluoride; D010749:Phosphoprotein Phosphatases