Comparative study of sister chromatid exchange induction and antitumor effects by homo-aza-steroidal esters of [p-[bis(2-chloroethyl)amino]phenyl]butyric acid.
The present work was undertaken in order to test the hypothesis that the Sister Chromatid Exchange (SCE) assay in vitro can be used for the prediction of in vivo tumor response to newly synthesized potential chemotherapeutics. The effect of three homo-aza-steroidal esters containing the -CONH- in the steroidal nucleus, 1, 2, and 3 on SCE rates and on cell kinetics in cultured human lymphocytes was studied. The antitumor activity of these compounds was tested on leukemia P388- and leukemia L1210-bearing mice. The three substances induced statistically significant enhancement of SCEs and of cell division delays. Compounds 1 and 3 were identified, on a molar basis, as more effective inducers of SCEs and of cell division delays compared with compound 2. Compounds 1 and 3 had upon both experimental tumors better therapeutic effects compared with compound 2 at equitoxic doses. Therefore, the order of the antitumor effectiveness of the three compounds coincided with the order of the cytogenetic effects they induced.
Laboratory of Pharmaceutical Chemistry, Patras, Greece.
C
C
Camoutsis
D
D
Catsoulacos
V
V
Karayiann
A
A
Papageorgiou
D
D
Mourelatos
E
E
Mioglou
Z
Z
Kritsi
S
S
Nikolaropoulos
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001378:Azasteroids / Q000627:therapeutic use*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D004273:DNA, Neoplasm / Q000378:metabolism; D004354:Drug Screening Assays, Antitumor; D005260:Female; D007939:Leukemia L1210 / Q000188:drug therapy* / Q000235:genetics; D007941:Leukemia P388 / Q000188:drug therapy* / Q000235:genetics; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D016896:Treatment Outcome
D000970:Antineoplastic Agents; D001378:Azasteroids; D004273:DNA, Neoplasm