[Photochemical synthesis of a bioaffinity sorbent for plasminogen extraction].
Conditions for affine sorbents creation by means of linear photocopolymer photochemical synthesis, its thermochemical granulation-crosslinking, and grafting by L-lysine hydrochloride as an sorbent affinant (ligand) were investigated. Linear photocopolymer properties depending on concentration ratio of photopolymerisation components, chemical nature of reaction media (solvent), and UV irradiation time were studied. Dioxane is shown to be the optimal solvent. Dependence of molecular mass on UV irradiation time (energy) is extremal with maximum at 5 hours and Mm = 243,300. Biospecific properties of several created affine sorbents were investigated.
Masliuk
A F
AF
Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv.
Kudinov
S A
SA
Bereznitskiĭ
G K
GK
Shikhanova
N A
NA
Shakhnin
D B
DB
Prokopova
E V
EV
D000345:Affinity Labels / Q000138:chemical synthesis* / Q000737:chemistry; D008970:Molecular Weight; D010777:Photochemistry; D010958:Plasminogen / Q000737:chemistry*; D014466:Ultraviolet Rays
D000345:Affinity Labels; D010958:Plasminogen