title: Identification of metallopanstimulin-1 as a member of a tumor associated antigen in patients with breast cancer.
abstract: The immunological screening of breast cancer was performed with IgG autoantibodies by the serological analysis of recombinant cDNA expression library methods to explore novel tumor associated antigens. We have focused on a small zinc finger protein metallopanstimulin-1 (MPS-1). MPS-1 mRNA was ubiquitously expressed in normal human tissues except the brain and the placenta. In Western blot analysis, MPS-1 was easily detected strongly in actively proliferating cells and three breast cancer cell lines. In the tissue the protein of MPS-1 in cancer cells was more abundant than that of surrounding normal cells. Screening of tissue specimens by immunohistochemistry revealed 50.4% positive for MPS-1 in 125 cancer patients. These data suggest that MPS-1 could be applicable to the immunotherapy of breast cancer.
authors.affiliation: Department of Pathology, Asahikawa Medical College, 2-1-1 Midorigaoka-higashi, Asahikawa 078-8510, Japan. atsutado@asahikawa-med-ac.jp
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Atsuta
authors.forename: Naoko
authors.initials: N
authors.lastname: Aoki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sato
authors.forename: Kensuke
authors.initials: K
authors.lastname: Oikawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nochi
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Miyokawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hirata
authors.forename: Shoji
authors.initials: S
authors.lastname: Kimura
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Sasajima
authors.forename: Makoto
authors.initials: M
authors.lastname: Katagiri
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D001943:Breast Neoplasms / Q000276:immunology* / Q000628:therapy; D018076:DNA, Complementary / Q000032:analysis; D005260:Female; D015723:Gene Library; D006801:Humans; D007150:Immunohistochemistry; D007167:Immunotherapy; D008667:Metalloproteins / Q000032:analysis* / Q000235:genetics; D009687:Nuclear Proteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D018076:DNA, Complementary; D008667:Metalloproteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C083686:RPS27 protein, human; D012269:Ribosomal Proteins