Frequent occurrence of CYP2D6*10 duplication allele in a Japanese population.
Allele-specific long-polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism (RFLP) and haplotype analysis using XbaI and EcoRI were used to determine whether gene duplication of CYP2D6*10 exists in a Japanese population of 162 healthy subjects. Based on the results of PCR and haplotype analysis, the frequencies of CYP2D6*1X2, CYP2D6*2X2 and CYP2D6*10X2 in the Japanese population were estimated to be 0.3, 0.3 and 0.6%, respectively. The results suggest that duplicated alleles of CYP2D6*10 exist in the Japanese population and that it may be one of the factors affecting the capacity of Japanese to metabolize various CYP2D6 substrate drugs.
Mitsunaga
Yoshiharu
Y
Department of Pharmacy, Faculty of Medicine, University of Tokyo Hospital, 7-3-1 Hongoh, Bunkyo-ku, Japan.
Kubota
Takahiro
T
Ishiguro
Akihiro
A
Yamada
Yasuhiko
Y
Sasaki
Hiroshi
H
Chiba
Kan
K
Iga
Tatsuji
T
D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D001711:Biotransformation / Q000235:genetics*; D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics*; D005260:Female; D020440:Gene Duplication; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D007564:Japan; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D007527:Isoenzymes; D019389:Cytochrome P-450 CYP2D6