Variation between two near isogenic barley (Hordeum vulgare) cultivars in expression of the B subunit of the vacuolar ATPase in response to salinity.
A gene encoding the barley vacuolar ATPase subunit B (BSVAP) was differentially expressed between two near isogenic barley cultivars, Golden Promise and Maythorpe. This gene (BSVAP) was isolated by the mRNA differential display technique (DDRT-PCR). BSVAP was salt inducible under long-term salinity stress in the salt sensitive cultivar Maythorpe but less so in the relatively salt tolerant Golden Promise and was more highly expressed under control conditions in Maythorpe. The physiological consequences of altered vacuolar ATPase expression are discussed in relation to the salt sensitivity of Maythorpe.
Wei
W
W
Biosciences Division, University of Wolverhampton, UK.
Bilsborrow
P
P
Hooley
P
P
Fincham
D
D
Forster
B
B
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004247:DNA / Q000096:biosynthesis; D020869:Gene Expression Profiling; D014644:Genetic Variation; D001467:Hordeum / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D012492:Salts / Q000494:pharmacology*; D017422:Sequence Analysis, DNA
D012333:RNA, Messenger; D012492:Salts; D004247:DNA; D000251:Adenosine Triphosphatases