title: Ultrastructural localization of orexin-1 receptor in pre- and post-synaptic neurons in the rat arcuate nucleus.
abstract: The distribution and ultrastructural localization of the orexin-1 receptor (OX(1)R) in the rat arcuate nucleus were studied using immunocytochemical techniques. OX(1)R-containing neurons were found throughout the nucleus, but were concentrated in its posterior region. Both OX(1)R-positive perikarya and dendrites were found to receive synapses from other unknown axon terminals. In addition, a small number of OX(1)R-positive axon terminals were observed. These OX(1)R-immunoreactive axon terminals were often found to make synapses on unidentified immunonegative dendritic processes. The present results indicate that orexin may act on food intake regulating neurons through both pre- and post-synaptic OX(1)R.
authors.affiliation: Department of Anatomy, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Jian-Lian
authors.initials: JL
authors.lastname: Guan
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Suzuki
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Funahashi
authors.forename: Qing-Ping
authors.initials: QP
authors.lastname: Wang
authors.forename: Haruaki
authors.initials: H
authors.lastname: Kageyama
authors.forename: Kichio
authors.initials: K
authors.lastname: Uehara
authors.forename: Shuori
authors.initials: S
authors.lastname: Yamada
authors.forename: Shinobu
authors.initials: S
authors.lastname: Tsurugano
authors.forename: Seiji
authors.initials: S
authors.lastname: Shioda
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000737:chemistry / Q000648:ultrastructure*; D008297:Male; D009474:Neurons / Q000737:chemistry / Q000648:ultrastructure*; D064446:Orexin Receptors; D017729:Presynaptic Terminals / Q000737:chemistry / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide / Q000032:analysis / Q000648:ultrastructure*
chemical_list: D064446:Orexin Receptors; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide