title: Preparation and Biological Activity of [B(1)Ala, B(2)Ala, B(3)Lys]-Insulin.
abstract: This paper describes the preparation and biological activity of an insulin analogue in which the B1-3 sequence (Phe-Val-Asn) of insulin is substituted by Ala-Ala-Lys. [B(1)Ala, B(2)Ala, B(3)Lys]-Insulin retains full in vivo activity and receptor binding activity as insulin, but its lipogenesis activity and immunoactivity are 70 % and 0.88 % of those of insulin respectively. The possible contribution of the N-terminus of B-chain of insulin to the structure and function of insulin is discussed.
authors.affiliation: Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. zhusq@sunm.shcnc.ac.cn
authors.forename: Ying
authors.initials: Y
authors.lastname: Ye
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Xu
authors.forename: Xin-Tang
authors.initials: XT
authors.lastname: Zhang
authors.forename: Shi-Yin
authors.initials: SY
authors.lastname: Yao
authors.forename: Shang-Quan
authors.initials: SQ
authors.lastname: Zhu