Genomic organization and transcription of satellite DNA in the ant Aphaenogaster subterranea (Hymenoptera, Formicidae).
A satellite DNA family (APSU) was isolated and characterized in the ant Aphaenogaster subterranea. This satellite DNA is organized in tandem repeats of 162 bp and is relatively AT rich (51.9%). Sequence analysis showed a high level of homogeneity between monomers. Loss of satellite DNA has been detected in queens in relation to workers, because the amount of satellite DNA in queens is about 25% of the amount found in workers. Restriction analysis of the total DNA with methylation-sensitive enzymes suggests that this DNA is not methylated. Analysis of the electrophoretic mobility of satellite DNA on non-denaturing polyacrylamide showed that this satellite DNA is only very lightly curved. Their possible transcription was analyzed using reverse transcription and polymerase chain reaction (RT-PCR). The satellite DNA is transcribed on the two DNA strands at the same level in worker and queen pupae, as well as in worker adults.
Lorite
P
P
Departamento de Biología Experimental, Universidad de Jaén, Spain.
Renault
S
S
Rouleux-Bonnin
F
F
Bigot
S
S
Periquet
G
G
Palomeque
T
T
D000818:Animals; D001000:Ants / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D004276:DNA, Satellite; D016678:Genome; D008969:Molecular Sequence Data; D011679:Pupa / Q000235:genetics; D014158:Transcription, Genetic
D004276:DNA, Satellite