title: Development of a new method to characterize (SMBV) antigen formulations using surface plasmon resonance technology.
abstract: Supra Molecular Biovectors (SMBV) are nanoparticles composed by a polysaccharidic core surrounded by a lipid bilayer. They are designed for drug delivery and vaccine and can be administrated by nasal route. The association rate and the stability of association between active principle (AP) or antigens (Ag) with SMBV can be evaluated using the plasmon resonance technology using a BIAcore X system and a HPA hydrophobic sensor chip. AP, Ag and/or adjuvant molecule solutions are injected over SMBV saturated HPA sensor chip surface. Using a very small quantity of material, this technique allows us to quickly have an overview of complex formulations using SMBV. It is also the fastest screening technique to select the best SMBV for each Ag and the best formulation process.
authors.affiliation: Biovector Therapeutics S.A., Chemin du Chene Vert, 31676 Cedex, Labege, France. jean_philippe.siguier@worldonline.fr
authors.forename: J P
authors.initials: JP
authors.lastname: Siguier
authors.forename: M
authors.initials: M
authors.lastname: Major
authors.forename: O
authors.initials: O
authors.lastname: Balland
mesh_terms: D000941:Antigens / Q000008:administration & dosage* / Q000737:chemistry; D015374:Biosensing Techniques; D002784:Cholesterol; D005079:Excipients; D008051:Lipid Bilayers; D008863:Microspheres; D010743:Phospholipids; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000941:Antigens; D005079:Excipients; D008051:Lipid Bilayers; D010743:Phospholipids; D002784:Cholesterol