Global analysis of stress-regulated mRNA turnover by using cDNA arrays.
cDNA array technology has proven to be a powerful way to monitor global changes in gene expression patterns. Here, we present an approach that extends the current utility of cDNA arrays to allow the evaluation of the relative roles of transcription and mRNA turnover in governing gene expression on a global basis, compared with current individual gene-by-gene analyses. This method, which involves comparison of large-scale hybridization patterns generated with steady-state mRNA versus newly transcribed (nuclear run-on) RNA, was used to demonstrate the importance of mRNA turnover in regulating gene expression following several conditions of stress.
Fan
Jinshui
J
Laboratory of Cellular and Molecular Biology and DNA Array Unit, National Institute on Aging-Intramural Research Program, National Institutes of Health, Baltimore, MD 21224-6825, USA.
Yang
Xiaoling
X
Wang
Wengong
W
Wood
William H
WH
Becker
Kevin G
KG
Gorospe
Myriam
M
D002289:Carcinoma, Non-Small-Cell Lung; D015870:Gene Expression; D018869:Heat-Shock Response; D006801:Humans; D008175:Lung Neoplasms; D020411:Oligonucleotide Array Sequence Analysis; D011454:Prostaglandins A / Q000494:pharmacology; D012333:RNA, Messenger; D013312:Stress, Physiological / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D011454:Prostaglandins A; D012333:RNA, Messenger; C100008:prostaglandin A2