title: Profiling of differentially expressed genes in human gastric carcinoma by cDNA expression array.
abstract: OBJECTIVE
  To investigate the expression of cancer related genes in gastric carcinoma (GC) through the use of Atlas Human Cancer Array membranes with 588 well-characterized human genes related to cancer and tumor biology.
  
  
  METHODS
  Hybridization of cDNA blotting membrane was performed with (32)P-labeled cDNA probes synthesized from RNA isolated from gastric carcinoma and adjacent noncancerous gastric epithelial tissue. AtlasImage, which is a software specific to array, was used to analyze the result.
  
  
  RESULTS
  The differentially expression cell cycle/growth regulator in GC showed a stronger tendency toward cell proliferation with 2.7-fold up-regulation of CK1. The promoter genes of apoptosis were down-regulated, including caspase-8 precursor, caspase-9 and caspase-10. Among the oncogene/tumor suppressor genes, ABL2 was down-regulated. In addition, some genes were up-regulated, including matrix metalloproteinse 2(MMP-2), MMP-16(MT3-MMP), SKY, CD9 and semaphorin V. A number of genes were down-regulated, including neuroendocrine-dlg (NE-dlg), retinoic acid receptor gamma and tumor suppressor DCC colorectal. In general, The expression of the cancer progression genes were up-regulated, while the expression of anti-cancer progression genes were down-regulated.
  
  
  CONCLUSIONS
  Investigation of these genes should help to disclose the molecular mechanism of the onset, progression and prognosis of GC. Several genes are reported herein to be altered in GC for the first time. The quick and high-throughout method of profiling gene expression by cDNA array provides us with an overview of key factors that may involved in GC, and may aid the study of GC carcinogenesis and provide molecular targets for diagnosis and therapy. The precise relationship between the altered genes and gastric carcinogenesis is a matter for further investigation.
authors.affiliation: Department of Surgery, the First Clinical College, Harbin Medical University, No.23 Youzheng Street, Nangang District, Harbin 150001, Heilongjiang Province, China. liulianxin@sohu.com
authors.forename: Lian-Xin
authors.initials: LX
authors.lastname: Liu
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Hong-Chi
authors.initials: HC
authors.lastname: Jiang
authors.forename: Xin
authors.initials: X
authors.lastname: Qu
authors.forename: Wei-Hui
authors.initials: WH
authors.lastname: Zhang
authors.forename: Lin-Feng
authors.initials: LF
authors.lastname: Wu
authors.forename: An-Long
authors.initials: AL
authors.lastname: Zhu
authors.forename: Xiu-Qin
authors.initials: XQ
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D015703:Antigens, CD / Q000235:genetics; D020169:Caspases / Q000235:genetics; D005753:Gastric Mucosa / Q000378:metabolism; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D006801:Humans; D007633:Keratins / Q000235:genetics; D020782:Matrix Metalloproteinases / Q000235:genetics; D008562:Membrane Glycoproteins; D020411:Oligonucleotide Array Sequence Analysis; D018168:Receptors, Retinoic Acid / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics*; D060245:Tetraspanin 29
chemical_list: D015703:Antigens, CD; C554869:CD9 protein, human; D008562:Membrane Glycoproteins; D018168:Receptors, Retinoic Acid; D060245:Tetraspanin 29; D007633:Keratins; D020169:Caspases; D020782:Matrix Metalloproteinases