Issues associated with and recommendations for using PCR to detect outbreaks of pertussis.
Two outbreaks of respiratory tract illness associated with prolonged cough occurring in 1998 and 1999 in New York State were investigated. A PCR test for Bordetella pertussis was primarily used by a private laboratory to confirm 680 pertussis cases. Several clinical specimens had positive culture results for B. pertussis during both outbreaks, which confirmed that B. pertussis was circulating during the outbreaks. However, testing by the New York State Department of Health reference laboratory suggested that some of the PCR results may have been falsely positive. In addition, features of the outbreak that suggested that B. pertussis may not have been the primary agent of infection included a low attack rate among incompletely vaccinated children and a significant amount of illness among patients testing PCR negative for B. pertussis. These investigations highlight the importance of appropriate clinical laboratory quality assurance programs, of the limitations of the PCR test, and of interpreting laboratory results in context of clinical disease.
Lievano
Fabio A
FA
Epidemic Intelligence Service, Epidemiology Program Office, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. flievano@cdc.gov
Reynolds
Meredith A
MA
Waring
Alfred L
AL
Ackelsberg
Joel
J
Bisgard
Kristine M
KM
Sanden
Gary N
GN
Guris
Dalya
D
Golaz
Anne
A
Bopp
Dianna J
DJ
Limberger
Ronald J
RJ
Smith
Perry F
PF
D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D005189:False Positive Reactions; D006801:Humans; D009518:New York / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*; D011786:Quality Control; D012015:Reference Standards; D013048:Specimen Handling; D014917:Whooping Cough / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology
D004269:DNA, Bacterial