title: Ca2+-ATPases (SERCA): energy transduction and heat production in transport ATPases.
abstract: The sarcoplasmic reticulum Ca2+-ATPase is able to cleave ATP through two different catalytic routes. In one of them, a part of the chemical energy derived from ATP hydrolysis is used to transport Ca2+ across the membrane and part is dissipated as heat. In the second route, the hydrolysis of ATP is completed before Ca2+ transport and all the energy derived from ATP hydrolysis is converted into heat. The second route is activated by the rise of the Ca2+ concentration in the vesicle lumen. In vesicles derived from white skeletal muscle the rate of the uncoupled ATPase is several-fold faster than the rate of the ATPase coupled to Ca2+ transport, and this accounts for both the low Ca2+/ATP ratio usually measured during transport and for the difference of heat produced during the hydrolysis of ATP by intact and leaky vesicles. Different drugs were found to selectively inhibit the uncoupled ATPase activity without modifying the activity coupled to Ca2+ transport. When the vesicles are actively loaded, part of the Ca2+ accumulated leaks to the medium through the ATPase. Heat is either produced or released during the leakage, depending on whether or not the Ca2+ efflux is coupled to the synthesis of ATP from ADP and Pi.
authors.affiliation: Departamento de Bioquímica Médica, Institute de Ciências Biomédicas, Centre de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Cidade Universitária, RJ, 21941 590, Brazil. demeis@bioqmed.ufjr.br
authors.forename: L
authors.initials: L
authors.lastname: de Meis
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D001693:Biological Transport, Active / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002384:Catalysis; D004721:Endoplasmic Reticulum / Q000201:enzymology; D004734:Energy Metabolism; D006358:Hot Temperature; D006868:Hydrolysis; D008954:Models, Biological; D008956:Models, Chemical; D012519:Sarcoplasmic Reticulum / Q000201:enzymology
chemical_list: D000255:Adenosine Triphosphate; D000252:Calcium-Transporting ATPases