Identification and characterization of a new family of C-type lectin-like genes from planaria Girardia tigrina.
A novel family of C-type lectin-like genes, denoted multidomain free lectin (MDFL), was identified in the freshwater planaria Girardia (Dugesia) tigrina. We cloned several genes that encode proteins comprising a signal peptide and a number of consecutive C-type lectin-like domains (CTLDs) interconnected by short linker stretches. Analyses of genomic organization, CTLD amino acid sequences, and the overall architecture of these proteins indicate that planarian proteins are a separate family of C-type lectin-like proteins. These genes are expressed in specifically differentiated gland cells of planaria and the corresponding proteins are excreted as components of the planarian body surface mucus.
Shagin
Dmitry A
DA
Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, 117871 Moscow, Russia.
Barsova
Ekaterina V
EV
Bogdanova
Ekaterina
E
Britanova
Olga V
OV
Gurskaya
Nadia
N
Lukyanov
Konstantin A
KA
Matz
Mikhail V
MV
Punkova
Natalia I
NI
Usman
Natalia Y
NY
Kopantzev
Eugene P
EP
Salo
Emili
E
Lukyanov
Sergey A
SA
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D017124:Conserved Sequence; D005091:Exons; D015801:Helminth Proteins / Q000737:chemistry; D007438:Introns; D037181:Lectins, C-Type / Q000737:chemistry / Q000235:genetics*; D008870:Microtubules / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009093:Mucus / Q000378:metabolism; D010802:Phylogeny; D010932:Planarians / Q000235:genetics* / Q000648:ultrastructure; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D015801:Helminth Proteins; D037181:Lectins, C-Type; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C111231:scarf protein, Dugesia tigrina; D002118:Calcium