Characterization of virus adsorption by using DEAE-sepharose and octyl-sepharoser.
Viruses were characterized by their adsorption to DEAE-Sepharose or by their elution from octyl-Sepharose by using buffered solutions of sodium chloride with different ionic strengths. Viruses whose adsorption to DEAE-Sepharose was reduced most rapidly by an increase in the sodium chloride concentration were considered to have the weakest electrostatic interactions with the solids; these viruses included MS2, E1, and phiX174. Viruses whose adsorption to DEAE-Sepharose was reduced least rapidly were considered to have the strongest electrostatic interactions with the column; these viruses included P1, T4, T2, and E5. All of the viruses studied adsorbed to octyl-Sepharose in the presence of 4 M NaCl. Viruses that were eluted most rapidly following a decrease in the concentration of NaCl were considered to have the weakest hydrophobic interactions with the column; these viruses included phiX174, CB4, and E1. Viruses that were eluted least rapidly from the columns after the NaCl concentration was decreased were considered to have the strongest hydrophobic interactions with the column; these viruses included f2, MS2, and E5.
Shields
Patricia A
PA
Department of Microbiology and Cell Science, The University of Florida, Gainesville, Florida 32611-0700, USA.
Farrah
Samuel R
SR
D000327:Adsorption; D000818:Animals; D004983:Ethanolamines; D009994:Osmolar Concentration; D012685:Sepharose / Q000031:analogs & derivatives* / Q000737:chemistry*; D012965:Sodium Chloride; D014780:Viruses
D004983:Ethanolamines; D012965:Sodium Chloride; C027176:octyl-sepharose CL-4B; D012685:Sepharose; C007369:2-diethylaminoethanol