[Heparin facilities proliferation of human osteoblasts in vitro].
It is a well-known fact that long-term application of heparin can lead to osteoporosis. To learn more about the mechanisms of heparin-induced osteoporosis, we exposed human osteoblasts in vitro to heparin in various concentrations. We found an increased proliferation rate, especially in concentrations used therapeutically in humans (0.1-0.2 IU/ml). In our experiments fetal calf serum (FCS) was able to heighten the positive effect of heparin, showing a synergism between heparin and FCS.
Matziolis
G
G
Unfallchirurgie, Universitätsklinikum, RWTH Aachen, Pauwelsstrasse 30, 52072 Aachen. Georg.Matziolis@Charite.de
Erli
H J
HJ
Rau
H M
HM
Klever
P
P
Bosserhoff
A K
AK
Paar
O
O
D002452:Cell Count; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D010006:Osteoblasts / Q000187:drug effects*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013268:Stimulation, Chemical
D012710:Serum Albumin, Bovine; D006493:Heparin