title: DNA analysis of nosocomial infection by Enterobacter aerogenes in three cases of septicaemia in Japan.
abstract: Ceftazidime-resistant Enterobacter aerogenes was isolated from blood cultures of three patients with fever. DNA analysis using pulsed-field gel electrophoresis and ribosomal RNA gene restriction digest pattern analysis revealed that the strains were clonally similar to each other with a 79.3-96.0% homology. The same strain of E. aerogenes was isolated from a three-way stopcock connected to the indwelling catheter in one of the patients at a concentration of 45 cfu/mL. A similar strain was also isolated from the urine of one other patient on the same floor. The data suggest that E. aerogenes caused septicaemia via low bacterial contamination of a three-way stopcock in a peripheral drip intravenous infusion system in at least one patient, and that the outbreak of E. aerogenes infections was due to clonally-related strains.
authors.affiliation: Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Goshi
authors.forename: I
authors.initials: I
authors.lastname: Taneike
authors.forename: S
authors.initials: S
authors.lastname: Nakagawa
authors.forename: S
authors.initials: S
authors.lastname: Kojio
authors.forename: Y
authors.initials: Y
authors.lastname: Tamura
authors.forename: T
authors.initials: T
authors.lastname: Ohara
authors.forename: K
authors.initials: K
authors.lastname: Ozaki
authors.forename: H
authors.initials: H
authors.lastname: Tsukada
authors.forename: Y
authors.initials: Y
authors.lastname: Aoki
authors.forename: H
authors.initials: H
authors.lastname: Asakura
authors.forename: F
authors.initials: F
authors.lastname: Gejyo
authors.forename: M
authors.initials: M
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000328:Adult; D003428:Cross Infection / Q000209:etiology*; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity; D004756:Enterobacteriaceae Infections / Q000209:etiology*; D004866:Equipment Contamination; D005260:Female; D006801:Humans; D007564:Japan; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D021521:Ribotyping / Q000379:methods*; D018805:Sepsis / Q000209:etiology*
chemical_list: D004269:DNA, Bacterial