In vivo detection of nitric oxide distribution in mice.
This paper discusses in vivo detection of nitric oxide (NO) distribution in endotoxin-treated mice using L-band (1.1 GHz) electron paramagnetic resonance spectroscopy (EPR) in combination with the hydrophilic NO trapping complex: N-methyl-D-glucamine dithiocarbamate and iron (MGD-Fe). MGD-Fe-NO complex is found in the upper abdomen (liver region), lower abdomen (kidney and urinary bladder) and head region of ICR mice. Experiments with nitric oxide synthase (NOS) inhibition and 15N-labeled L-arginine as NOS substrate verify the origin of trapped NO from L-arginine. However, contribution from a 'nonenzymatic' NO generation pathway can not be ruled out. This paper further examines potential artifacts, which may arise in experiments using dithiocarbamate-iron complexes as NO trapping agents.
Komarov
Andrei M
AM
Department of Physiology and Experimental Medicine, The George Washington University Medical Center, Washington, DC 20037, USA. phyamk@gwumc.edu
D000005:Abdomen; D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D004731:Endotoxins / Q000494:pharmacology; D005296:Ferrous Compounds / Q000737:chemistry; D006257:Head; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000032:analysis*; D013012:Sorbitol / Q000031:analogs & derivatives* / Q000737:chemistry; D013113:Spin Labels; D013859:Thiocarbamates / Q000737:chemistry
D004731:Endotoxins; D005296:Ferrous Compounds; D013113:Spin Labels; D013859:Thiocarbamates; C110934:dithiocarbamate-Fe(II); D009569:Nitric Oxide; D013012:Sorbitol; C041262:N-methyl-D-glucamine dithiocarbamate