title: Identification and sequencing of HLA-B*0714 and B*2718 alleles and novel exon 1 sequences of B*0709 and B*2714 alleles in potential bone marrow donors.
abstract: Sequence specific oligonucleotide probe hybridization and sequence specific primer PCR typing of volunteer bone marrow donors suggested the presence of variants of known HLA-B alleles in two individuals. PCR products encompassing HLA-B locus exons 1, 2, and 3 were prepared, subcloned and sequenced. A Hispanic individual had a novel B*07 allele (B*0714) and a Chinese individual had a novel B*27 allele (B*2718). In two other individuals, a previously unknown sequence of exon 1 was determined for HLA-B*0709 (African American) and B*2714 (Native American). These findings further illustrate the substantial genetic variation present at the HLA-B locus within human populations. We discuss the structural variation in the protein sequence for these HLA-B alleles and its potential functional effects.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: D
authors.initials: D
authors.lastname: Feldman
authors.forename: J
authors.initials: J
authors.lastname: Kearns
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: S
authors.initials: S
authors.lastname: Bassinger
authors.forename: G D
authors.initials: GD
authors.lastname: Montoya
authors.forename: M
authors.initials: M
authors.lastname: McKeen
authors.forename: S
authors.initials: S
authors.lastname: Birkos
authors.forename: T M
authors.initials: TM
authors.lastname: Williams
authors.forename: M
authors.initials: M
authors.lastname: Kamoun
mesh_terms: D044466:Asian People / Q000235:genetics; D044383:Black People / Q000235:genetics; D016026:Bone Marrow Transplantation / Q000276:immunology*; D005091:Exons; D015235:HLA-B Antigens / Q000235:genetics*; D006630:Hispanic or Latino / Q000235:genetics; D006801:Humans; D007198:Indians, North American / Q000235:genetics; D014019:Tissue Donors; D001741:Black or African American
chemical_list: D015235:HLA-B Antigens