title: Novel evidence that nitric oxide of the medial septal area influences the salivary secretion induced by pilocarpine.
abstract: Our studies have focused on the effect of injection of L-NAME and sodium nitroprussiate (SNP) on the salivary secretion, arterial blood pressure, sodium excretion and urinary volume induced by pilocarpine which was injected into the medial septal area (MSA). Rats were anesthetized with urethane (1.25 g/kg b. wt.) and a stainless steel cannula was implanted into their MSA. The amount of saliva secretion was studied over a five-minute period after injection of pilocarpine into MSA. Injection of pilocarpine (10, 20, 40, 80, 160 microg/microl) into MSA produced a dose-dependent increase in salivary secretion. L-NG-nitro arginine methyl-esther (L-NAME) (40 microg/microl), a nitric oxide (NO) synthase inhibitor, was injected into MSA prior to the injection of pilocarpine into MSA, producing an increase in salivary secretion due to the effect of pilocarpine. Sodium nitroprussiate (SNP) (30 microg/microl) was injected into MSA prior to the injection of pilocarpine into MSA attenuating the increase in salivary secretion induced by pilocarpine. Medial arterial pressure (MAP) increase after injections of pilocarpine into the MSA. L-NAME injected into the MSA prior to injection of pilocarpine into MSA increased the MAP. SNP injected into the MSA prior to pilocarpine attenuated the effect of pilocarpine on MAP. Pilocarpine (40 ug/ul) injected into the MAS induced an increase in sodium and urinary excretion. L-NAME injected prior to pilocarpine into the MSA increased the urinary sodium excretion and urinary volume induced by pilocarpine. SNP injected prior to pilocarpine into the MSA decreased the sodium excretion and urinary volume induced by pilocarpine. All these roles of pilocarpine depend on the release of nitric oxide into the MSA. We may also conclude that the MSA is involved with the cholinergic excitatory mechanism that induce salivary secretion, increase in MAP and increase in sodium excretion and urinary volume.
authors.affiliation: Department of Physiology and Pathology School of Dentistry, Paulista State University, UNESP Araraquara, SP Brazil. silvana@foar.unesp.br
authors.forename: Wilson Abrão
authors.initials: WA
authors.lastname: Saad
authors.forename: Ismael Francisco Motta Siqueira
authors.initials: IF
authors.lastname: Guarda
authors.forename: Luiz Antonio de Arruda
authors.initials: LA
authors.lastname: Camargo
authors.forename: Talmir Augusto Faria Brisola
authors.initials: TA
authors.lastname: dos Santos
authors.forename: William Abrão
authors.initials: WA
authors.lastname: Saad
authors.forename: Sylvio
authors.initials: S
authors.lastname: Simões
authors.forename: Renata Saad
authors.initials: RS
authors.lastname: Guarda
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006439:Hemodynamics / Q000187:drug effects; D007267:Injections; D007668:Kidney / Q000187:drug effects; D008297:Male; D018721:Muscarinic Agonists / Q000008:administration & dosage / Q000494:pharmacology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009599:Nitroprusside / Q000494:pharmacology; D010862:Pilocarpine / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012472:Salivation / Q000187:drug effects*; D020665:Septum of Brain / Q000502:physiology*; D012964:Sodium / Q000097:blood / Q000652:urine; D014563:Urodynamics / Q000187:drug effects
chemical_list: D004791:Enzyme Inhibitors; D018721:Muscarinic Agonists; D010862:Pilocarpine; D009599:Nitroprusside; D009569:Nitric Oxide; D012964:Sodium; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester