Parallel identification of new genes in Saccharomyces cerevisiae.
Short open reading frames (ORFs) occur frequently in primary genome sequence. Distinguishing bona fide small genes from the tens of thousands of short ORFs is one of the most challenging aspects of genome annotation. Direct experimental evidence is often required. Here we use a combination of expression profiling and mass spectrometry to verify the independent transcription of 138 and the translation of 50 previously nonannotated genes in the Saccharomyces cerevisiae genome. Through combined evidence, we propose the addition of 62 new genes to the genome and provide experimental support for the inclusion of 10 previously identified genes.
Oshiro
Guy
G
Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA.
Wodicka
Lisa M
LM
Washburn
Michael P
MP
Yates
John R
JR
Lockhart
David J
DJ
Winzeler
Elizabeth A
EA
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics; D019295:Computational Biology / Q000379:methods; D017124:Conserved Sequence / Q000235:genetics / Q000502:physiology; D030541:Databases, Genetic / Q000145:classification; D029721:Drosophila Proteins / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D005800:Genes, Fungal / Q000235:genetics* / Q000502:physiology; D016681:Genome, Fungal; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics / Q000502:physiology; D010455:Peptides / Q000032:analysis / Q000502:physiology; D014176:Protein Biosynthesis / Q000235:genetics / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000502:physiology
D029721:Drosophila Proteins; D010455:Peptides; D029701:Saccharomyces cerevisiae Proteins