title: Saliva IgM and IgA are a sensitive indicator of the humoral immune response to Escherichia coli O157 lipopolysaccharide in children with enteropathic hemolytic uremic syndrome.
abstract: Saliva antibodies to Escherichia coli O157 were investigated as markers of the immune response in children with enteropathic hemolytic uremic syndrome (HUS). Paired serum and saliva samples were collected from 22 children with HUS during acute disease and convalescence and were tested for E. coli O157 lipopolysaccharide (LPS)-specific IgM and IgA antibodies by ELISA. Serum and saliva samples from 44 age-matched controls were used to establish the cut-off values. Elevated levels of IgM and/or IgA antibodies to O157 LPS were detected in saliva of 13/13 HUS patients with Shiga toxin-producing E. coli (STEC) O157 in stool culture and from 4 of 5 HUS patients in whom STEC were not detected. These results closely mirrored the results obtained with paired serum samples. In contrast, saliva and serum samples from four children with STEC isolates belonging to O-groups O26, O145 (n = 2), and O165 lacked detectable O157 LPS-specific antibodies. The specificity of the ELISA was confirmed by western blotting. In STEC O157 culture-confirmed cases, the sensitivity of the ELISA was 92% for saliva IgM and IgA, based on the first available sample, and 100% and 92%, respectively, when subsequent samples were included. The specificity was 98% for IgM and 100% for IgA. Children with E. coli O157 HUS demonstrate a brisk, easily detectable immune response as reflected by the presence of specific antibodies in their saliva. Saliva-based immunoassays offer a reliable, noninvasive method for the diagnosis of E. coli O157 infection in patients with enteropathic HUS.
authors.affiliation: Klinik und Poliklinik für Kinder- und Jugendmedizin, Universtität Hamburg, Universitätsklinikum Hamburg-Eppendorf, Germany. kludwig@uke.uni-hamburg.de
authors.forename: Kerstin
authors.initials: K
authors.lastname: Ludwig
authors.forename: Enke
authors.initials: E
authors.lastname: Grabhorn
authors.forename: Martin
authors.initials: M
authors.lastname: Bitzan
authors.forename: Christoph
authors.initials: C
authors.lastname: Bobrowski
authors.forename: Markus J
authors.initials: MJ
authors.lastname: Kemper
authors.forename: Ingo
authors.initials: I
authors.lastname: Sobottka
authors.forename: Rainer
authors.initials: R
authors.lastname: Laufs
authors.forename: Helge
authors.initials: H
authors.lastname: Karch
authors.forename: Dirk E
authors.initials: DE
authors.lastname: Müller-Wiefel
mesh_terms: D000293:Adolescent; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D000917:Antibody Formation; D002648:Child; D002675:Child, Preschool; D004731:Endotoxins / Q000276:immunology; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000276:immunology; D019453:Escherichia coli O157 / Q000276:immunology*; D005243:Feces / Q000382:microbiology; D006463:Hemolytic-Uremic Syndrome / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D007223:Infant; D012463:Saliva / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D004731:Endotoxins; D007070:Immunoglobulin A; D007075:Immunoglobulin M; C044101:endotoxin, Escherichia coli