title: An electrochemical adaptation of Ellman's test.
abstract: The electrochemically initiated reaction of thiols with N,N-diethyl-p-phenylenediamine has been coupled with an existing colorimetric sensing reaction developed by Ellman as a means of providing an electrochemical adaptation of the latter whereby the total thiol species present in a sample can be determined. The detection methodology has been proven to be robust with a linear range for cysteine from 2-120 microM, a limit of detection of 1.17 microM and has shown selectivity against a wide range of potential interferences. The efficiency of the methodology has been examined in the determination and recovery of thiol species in three growth tissue media, which contain a number of common biological interferences.
authors.affiliation: Physical and Theoretical Chemistry Laboratory, University of Oxford, UK.
authors.forename: Olga
authors.initials: O
authors.lastname: Nekrassova
authors.forename: Paul C
authors.initials: PC
authors.lastname: White
authors.forename: Sarah
authors.initials: S
authors.lastname: Threlfell
authors.forename: Geraldine
authors.initials: G
authors.lastname: Hignett
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Wain
authors.forename: Nathan S
authors.initials: NS
authors.lastname: Lawrence
authors.forename: James
authors.initials: J
authors.lastname: Davis
authors.forename: Richard G
authors.initials: RG
authors.lastname: Compton