Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination.
We examined the formation of complexes between terbium ion (Tb3+) and carbohydrates labeled with aminobenzene compounds. Of the examined compounds, carbohydrates labeled with 4-aminosalicylic acid showed intense fluorescence with Tb3+ in the presence of cetylpyridinium chloride at pH 6.0. Calibration curves for maltose derivative showed good linearity between 5 pmol and at least 600 pmol, with good reproducibility. We applied the proposed technique to binding studies between manno-oligosaccharides and Concanavalin A.
Nakajima
Kazuki
K
Faculty of Pharmaceutical Sciences, Kinki University, Higashi-osaka, Japan.
Oda
Yasuo
Y
Kinoshita
Mitsuhiro
M
Masuko
Takashi
T
Kakehi
Kazuaki
K
D000818:Animals; D003208:Concanavalin A / Q000378:metabolism*; D005470:Fluorometry / Q000379:methods; D009844:Oligosaccharides / Q000378:metabolism*; D011485:Protein Binding; D013725:Terbium / Q000378:metabolism; D013997:Time Factors
D009844:Oligosaccharides; D013725:Terbium; D003208:Concanavalin A