title: A prionogenic peptide derived from Sup35 can force the whole GST fusion protein to show amyloid characteristics.
abstract: A prion determining 7-mer peptide derived from Sup35 was fused to glutathione S transferase (GST). The fusion protein was successfully overexpressed in Escherichia coli, and purified by employing affinity chromatography. Upon incubation, it showed substantial aggregation suggesting the formation of amyloid-like fibrils. Congo Red binding strongly suggested that the fusion protein formed amyloid-like fibrils. By considering the steric hindrance of GST, the beta-sheet formation should be in the anti-parallel fashion.
authors.affiliation: Department of Applied Chemistry and Recombinant Protein Expression Center (RPEC), Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul, 143-747, Korea.
authors.forename: Young Kee
authors.initials: YK
authors.lastname: Chae
authors.forename: Kyoung Suk
authors.initials: KS
authors.lastname: Cho
authors.forename: Woochun
authors.initials: W
authors.lastname: Chun
mesh_terms: D004396:Coloring Agents / Q000378:metabolism; D003224:Congo Red / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter; D010454:Peptide Termination Factors; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011328:Prions / Q000378:metabolism*; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D004396:Coloring Agents; D005656:Fungal Proteins; D010454:Peptide Termination Factors; D010455:Peptides; D011328:Prions; D011993:Recombinant Fusion Proteins; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D003224:Congo Red