title: Effects of dexamethasone on mucin gene expression in cultured human nasal epithelial cells.
abstract: OBJECTIVE
  To clarify 1) which mucin gene expression is influenced by glucocorticoid and 2) whether glucocorticoid influences steady-state mucin expression or mucin gene expression induced by lipopolysaccharide.
  
  
  METHODS
  Dissociated cells obtained from nasal polyps were cultured on a collagen gel substrate in an air-liquid interface. Dexamethasone was added to the culture medium in the steady-state and prior to the stimulation by LPS. RNAs were extracted from culture cells, and semiquantitative reverse transcriptase-polymerase chain reaction was performed for MUC2, MUC5AC, MUC5B, MUC8, and beta-actin.
  
  
  RESULTS
  Dexamethasone did not influence steady-state messenger RNA levels of either mucin gene. Dexamethasone suppressed lipopolysaccharide-induced messenger RNA levels of MUC8.
  
  
  CONCLUSIONS
  Glucocorticoid does not influence steady-state mucin gene expression but suppresses mucin gene expression induced by secretagogues such as lipopolysaccharide.
authors.affiliation: Department of Otorhinolaryngology, Mie University School of Medicine, Tsu, Mie, Japan. hajime@clin.med.mie-u.ac.jp
authors.forename: Hajime
authors.initials: H
authors.lastname: Ishinaga
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Chikako
authors.initials: C
authors.lastname: Kishioka
authors.forename: Mayuko
authors.initials: M
authors.lastname: Yagawa
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Majima
mesh_terms: D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D009077:Mucins / Q000187:drug effects* / Q000235:genetics*; D009297:Nasal Mucosa / Q000166:cytology* / Q000187:drug effects*
chemical_list: D005938:Glucocorticoids; D008070:Lipopolysaccharides; D009077:Mucins; D003907:Dexamethasone