[Growth stimulation of human osteoblast-like cells by thrombocyte concentrates in vitro].
OBJECTIVE
Thrombocytes contain different growth factors. Their potential use for increasing bone regeneration is currently under discussion. Basic data about the biologic effect of platelet concentrates (platelet-rich plasma, PRP) are still lacking. This study analyzes the in vitro effect of PRP on the proliferation rate of human osteoblast-like cells (OLC).


METHODS
Human OLC were stimulated with thrombocytes in different dilutions (0-1,700,000 thrombocytes/microliter). Three days later a WST test (Roche Diagnostics) was performed to measure the mitochondrial syntheses and cellular proliferation rate.


RESULTS
The proliferation rate of the OLC was (concentration-dependent) increased up to a certain plateau by adding thrombocytes. Further stimulation led to a slight decrease in the proliferation rate. The statistical analysis of the extinction measurements showed significant differences in the cell proliferation rates between mostly all thrombocyte concentration groups (p < 0.0001). Moreover, a statistically significant difference was evaluated for the time-dependent change in extinction (0-5 h) (p < 0.0001).


CONCLUSIONS
This study shows that the proliferation rate of human OLC can be stimulated in vitro by concentration-dependent platelet concentrates. This in vitro result supports the currently discussed assumption that the clinical use of PRP might increase bone regeneration.
Weibrich
G
G
Klinik für Mund-, Kiefer- und Gesichtschirurgie, Universitätsklinik Mainz, Augustusplatz 2, 55131 Mainz. weibrich@mkg.klinik.uni-mainz.de
Gnoth
S H
SH
Otto
M
M
Reichert
T E
TE
Wagner
W
W
D001861:Bone Regeneration / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D010006:Osteoblasts / Q000187:drug effects*; D010976:Platelet Count; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*
D010982:Platelet-Derived Growth Factor