Autologous endometrial coculture in patients with a previous history of poor quality embryos.
OBJECTIVE
To evaluate the effect of autologous endometrial coculture in patients (less than 36 years old) with a history of a single IVF failed cycle associated with poor quality embryos.


METHODS



METHODS
Controlled clinical study.


METHODS
University-based in vitro fertilization center.


METHODS
Twenty-six patients with a history of a single prior failed IVF-ET with poor preembryo quality.


METHODS
Autologous endometrial coculture.


METHODS
Preembryo blastomere numbers and cytoplasmic fragmentation rates were compared between the treatment and previous cycle. Clinical pregnancy rates were analyzed.


RESULTS
Twenty-six women with an average age of 32.8 +/- 2.9 years underwent treatment. On Day 3 the overall mean number of blastomeres per preembryo on coculture compared to conventional medium in a previous cycle was 6.1 +/- 1.8 vs. 5.1 +/- 1.3 (P = 0.01; Wilcoxon test). The average percentage of cytoplasmic fragments on coculture compared to the conventional medium in a previous cycle was 14% +/- 10 vs. 22% +/- 13 (P = 0.003; Wilcoxon test). At transfer the mean number of blastomeres per preembryo on coculture was 7.4 +/- 1.8 compared to 6.7 +/- 1.5 on conventional medium in a previous cycle (P = 0.02; Wilcoxon test). The clinical pregnancy rate (positive fetal cardiac activity) per patient was 88.5%. The delivery rate was 73.1% (19/26).


CONCLUSIONS
There was an improvement in the preembryo quality for preembryos on autologous endometrial coculture compared to noncocultured preembryos from the same patient in a previous cycle. An excellent delivery rate was subsequently found.
Spandorfer
Steven D
SD
The Center For Reproductive Medicine and Infertility, The New York Hospital, Cornell Medical Center, New York 10021, USA.
Barmat
Laffy
L
Navarro
Jose
J
Burmeister
Lynn
L
Veeck
Lucinda
L
Clarke
Robert
R
Liu
Hung-Ching
HC
Rosenwaks
Zev
Z
D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods; D018920:Coculture Techniques / Q000379:methods; D004622:Embryo, Mammalian; D004717:Endometrium / Q000166:cytology / Q000254:growth & development*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D011247:Pregnancy; D011256:Pregnancy Outcome