Production of recombinant human calcitonin from silkworm (B. mori) larvae infected by baculovirus.
A synthetic modified gene encoding the human calcitonin analog (hmCT) was expressed by use of the baculovirus expression system. After injection with recombinant baculoviruses, the hmCT-GST fusion protein was produced within the silkworm larvae. The fusion protein was purified by affinity chromatography. Biological activity of hmCT for hypercalcemic effect was determined in normal rats.
Yang
Guan-Zhen
GZ
Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Science, Shanghai, 200031, China.
Chen
Zhen-zhen
ZZ
Cui
Da-Fu
DF
Li
Bo-liang
BL
Wu
Xiang-fu
XF
D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000502:physiology*; D012831:Bombyx / Q000235:genetics / Q000502:physiology* / Q000821:virology; D002116:Calcitonin / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D017930:Genes, Reporter; D006458:Hemolymph / Q000737:chemistry; D006801:Humans; D006934:Hypercalcemia / Q000139:chemically induced; D007814:Larva / Q000502:physiology; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology
D011993:Recombinant Fusion Proteins; D002116:Calcitonin