Isoserine-based biotinylated photoaffinity probes that interact with penicillin-binding protein 1b.
The photolytic decomposition of trifunctional carbene generating photoaffinity probes in methanolic solution was studied, a cleavage reaction with butylamine in water, the conjugation with a ligand (moenomycin), and experiments that demonstrate that the fully armed probes interact with penicillin-binding protein 1b.
Rühl
Thomas
T
Institut für Organische Chemie, Universität Leipzig, Johannisalle 29, D-04103 Leipzig, Germany.
Volke
Daniela
D
Stembera
Katherina
K
Hatanaka
Yasumaru
Y
Hennig
Horst
H
Schumer
Frank
F
Welzel
Peter
P
D001426:Bacterial Proteins; D001710:Biotin / Q000737:chemistry*; D002082:Butylamines; D002352:Carrier Proteins; D006602:Hexosyltransferases / Q000737:chemistry*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D009097:Multienzyme Complexes / Q000737:chemistry*; D002267:Muramoylpentapeptide Carboxypeptidase; D009844:Oligosaccharides / Q000737:chemistry; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000737:chemistry*; D019920:Photoaffinity Labels; D010777:Photochemistry; D010782:Photolysis; D012694:Serine / Q000031:analogs & derivatives* / Q000737:chemistry*
D001426:Bacterial Proteins; D002082:Butylamines; D002352:Carrier Proteins; D007202:Indicators and Reagents; D009097:Multienzyme Complexes; D009844:Oligosaccharides; D046915:Penicillin-Binding Proteins; D019920:Photoaffinity Labels; D012694:Serine; C072421:isoserine; D001710:Biotin; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; C419802:moenomycin