The Th1-specific costimulatory molecule, m150, is a posttranslational isoform of lysosome-associated membrane protein-1.
In an earlier report, we had shown a 150-kDa protein termed as M150, isolated from the surface of activated macrophages, to possess costimulatory activity for CD4(+) T cells. Significantly, this protein was found to specifically elicit Th1 responses. In this study, we characterize M150, which belongs to a unique subset of the lysosome-associated membrane protein-1 glycoprotein. Interestingly, the costimulatory activity of M150 depends on its posttranslational modification, which has a distinct glycosylation pattern restricted to macrophages. Furthermore, it has been demonstrated that in addition to stimulating Th1-specific responses, M150 is also capable of driving differentiation of naive CD4(+) T cells into the Th1 subset. This altered posttranslational modification of housekeeping protein appears to represent a novel pathway by which APCs can additionally regulate T cell responses.
Prasad
Durbaka V R
DV
National Centre For Cell Science, Pune, India.
Parekh
Vrajesh V
VV
Joshi
Bimba N
BN
Banerjee
Pinaki P
PP
Parab
Pradeep B
PB
Chattopadhyay
Samit
S
Kumar
Anil
A
Mishra
Gyan C
GC
D000595:Amino Acid Sequence; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D001483:Base Sequence; D005260:Female; D015870:Gene Expression; D006031:Glycosylation; D051907:Lysosomal Membrane Proteins; D008247:Lysosomes / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D020825:T-Box Domain Proteins; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics; D000098815:T-bet Transcription Factor
D015703:Antigens, CD; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D014157:Transcription Factors