Effect of Type I Collagen on the Susceptibility of Low Density Lipoprotein to Oxidation.
In order to observe the effect of type I collagen on the susceptibility of low density lipoprotein (LDL) to oxidation, the collagen gel system was established in vitro. The presence of collagen resulted in a protracted lag phase as well as a decreased production of the thiobarbituric acid reactive substance (TBARS) when LDL was oxidized by the addition of copper ion. The relative electrophoretic mobilities (REM) of LDL in the treated group were far lower than those in the control group; while during the 2,2'-amidinopropane hydrochloride(AAPH)-catalyzed oxidation of LDL, neither the lag phase nor the maximum content of the output of TBARS were significantly affected by the presence of collagen. These results suggest that collagen may decrease the susceptibility of LDL to copper-catalyzed oxidation the mechanism of which may not involve the capture of free radicals.
Wei
En-Hui
EH
Atherosclerosis Research Center, Nanjing Medical University, Nanjing 210029, China.
Chen
Qi
Q
Chen
Xiu-Ying
XY
Wang
Nan
N
Shi
Xin-Feng
XF