[Genetic map of 10 STR loci on chromosome 9].
To understand the genetic polymorphism of 10 STR loci on chromosome 9 and reveal the difference on the linkage maps of different populations, the author used a set of nine tetranucleotide- and one tritranucleotide-repeat STR markers on chromosome 9 chosen from Genome Data Bank. EDTA-blood was collected from unrelated family in Baiyin City, Gansu province. DNA was extracted by Chelex-100 method and amplified by the polymerase chain reaction (PCR). The PCR products were analyzed using PAGE electrophoresis. We calculated the allele frequencies and heterozygosity and PIC of every ten STR loci. Using the data of Chinese Han pedigree, we constructed a genetic map of chromosome 9 making up with 10 STR loci. Then we compared our map with the map in GDB. Our results indicate that there are some differences on the genetic map between Chinese and GDB. That means, it is essential to do some population genetic evaluations before using the data of GDB into Chinese population.
Zhang
Ji
J
School of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Hou
Yi-Ping
YP
Li
Ying-Bi
YB
Wu
Jin
J
Wang
Yu-Fang
YF
D002681:China / Q000208:ethnology; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D008040:Genetic Linkage; D006801:Humans; D020080:Tandem Repeat Sequences