Neutrophil chemotaxis in bile duct-obstructed rats, and effect of internal biliary drainage.
OBJECTIVE
Our previous studies demonstrated enhanced neutrophil chemotaxis in bile duct-ligated, obstructive jaundice rats. In the present study, we produced a reversible obstructive jaundice model in rats. The efficacy of the present model in producing sufficient bile flow blockade and subsequent internal biliary drainage was assessed. Furthermore, the effect of internal biliary drainage on neutrophil chemotaxis was evaluated.


METHODS
Bile duct was obstructed with a polyester tape attached with a stainless steel coil. Internal biliary drainage was performed by removing the tape. Rats were subjected to either 10 days' bile duct obstruction or 4 days' bile duct obstruction followed by 6 days' internal biliary drainage. Some animals underwent conventional bile duct ligation and dissection for 4 or 10 days. Neutrophil chemotaxis was evaluated with a modified Boyden method using interleukin-8 (recombinant rat Gro-beta) as chemoattractant.


RESULTS
The present technique produced sufficient obstructive jaundice as evidenced by increases in serum alanine aminotransferase and total bilirubin throughout the observation period, the values of which were insignificant with those induced by the conventional method. Internal biliary drainage effectively normalized these values. Similarly, neutrophil chemotaxis was enhanced with both procedures, and increased neutrophil chemotaxis was significantly decreased after drainage.


CONCLUSIONS
The present reversible obstructive jaundice method is as efficacious as the conventional method for producing obstructive jaundice, and internal biliary drainage could be readily available. With the present model, neutrophil overactivity in obstructive jaundice was effectively alleviated by internal biliary drainage. The result may support the role of preoperative biliary drainage in the prevention of postoperative septic complications.
Shibatani
Nobuyuki
N
Third Department of Internal Medicine, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka 570-8506, Japan.
Yamamoto
Shin
S
Kubota
Yoshitsugu
Y
Tsuji
Kazuyuki
K
Takaoka
Makoto
M
Amoh
Yasuo
Y
Matsushita
Masanori
M
Shimatani
Masaaki
M
Imai
Yoshihito
Y
Inoue
Kyoichi
K
D000818:Animals; D001653:Bile Ducts, Intrahepatic / Q000276:immunology / Q000473:pathology; D002455:Cell Division / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D002780:Cholestasis, Intrahepatic / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D004322:Drainage / Q000379:methods*; D008111:Liver Function Tests; D008297:Male; D009504:Neutrophils / Q000276:immunology* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome