Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter.
The orchid DSCKX1 is a new member of the cytokinin oxidase gene family, which catalyses the degradation of cytokinins bearing unsaturated isoprenoid side chains. A 3.7 kb fragment upstream of the DSCKX1 coding region was isolated, sequenced and characterized by deletion analysis of DSCKX1::beta-glucuronidase gene fusions using transient orchid and stable Arabidopsis transformation systems. Functional analysis of 5' deletions defined the 5'-upstream region that directs the expression in distinct tissues. Regulatory elements affecting the cytokinin induction of the DSCKX1 gene have also been delineated
Yang
Shu Hua
SH
Plant Growth and Development Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Republic of Singapore.
Yu
Hao
H
Goh
Chong Jin
CJ
D024506:5' Flanking Region / Q000235:genetics; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D003583:Cytokinins / Q000494:pharmacology; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000201:enzymology* / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification; D018514:Plant Structures / Q000187:drug effects / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion
D003583:Cytokinins; D018744:DNA, Plant; D011993:Recombinant Fusion Proteins; D010088:Oxidoreductases; C019984:cytokinin oxidase; D005966:Glucuronidase