Impact of genomic technologies on studies of bacterial gene expression.
The ability to simultaneously monitor expression of all genes in any bacterium whose genome has been sequenced has only recently become available. This requires not only careful experimentation but also that voluminous data be organized and interpreted. Here we review the emerging technologies that are impacting the study of bacterial global regulatory mechanisms with a view toward discussing both perceived best practices and the current state of the art. To do this, we concentrate upon examples using Escherichia coli and Bacillus subtilis because prior work in these organisms provides a sound basis for comparison.
Rhodius
Virgil
V
Department of Stomatology, University of California, San Francisco, 94143, USA. rhodius@itsa.ucsf.edu
Van Dyk
Tina K
TK
Gross
Carol
C
LaRossa
Robert A
RA
D019677:Artificial Gene Fusion / Q000379:methods; D016000:Cluster Analysis; D019992:Databases as Topic / Q000145:classification*; D015964:Gene Expression Regulation, Bacterial; D023281:Genomics / Q000379:methods*; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods