Isotopically chiral probes for in situ high-throughput asymmetric reaction analysis.
In this work, we disclose a high-throughput screening assay for in situ analysis of enantioselection. The assay makes use of enantioenriched probe substrates that are isotopically chiral by virtue of a 13C isotope label. Upon catalytic asymmetric transformation, the carbon labels in the pseudoenantiomeric reaction products are in alternate diastereotopic environments and therefore lead to unique resonances. Simple integration allows for calculation of conversion and enantioselection.
Evans
Michael A
MA
Department of Chemistry, Venable Laboratory, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3290, USA.
Morken
James P
JP
D002384:Catalysis; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008968:Molecular Conformation; D019151:Peptide Library; D013237:Stereoisomerism
D007202:Indicators and Reagents; D019151:Peptide Library