The antibacterial drug taurolidine induces apoptosis by a mitochondrial cytochrome c-dependent mechanism.
The antibacterial agent taurolidine (TRD) has been used as a lavage antibiotic to prevent development of peritonitis in patients after surgery. We recently showed that TRD induced growth arrest and apoptosis of a variety of cultured cell lines derived from human solid tumors and also significantly inhibited the growth of human ovarian tumors in a mouse model. In this report, we present data to show that TRD, at concentrations below the doses that are used to treat patients in the clinic, induces apoptosis of human leukemia HL-60 cells by a mitochondrial cytochrome c-dependent pathway.
Han
Zhiyong
Z
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA. zhiyong_han@brown.edu
Ribbizi
Iole
I
Pantazis
Panayotis
P
Wyche
James
J
Darnowski
James
J
Calabresi
Paul
P
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D061945:Caspase Inhibitors; D002460:Cell Line; D003574:Cytochrome c Group / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D019254:Genes, bcl-2; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D008928:Mitochondria / Q000187:drug effects*; D013654:Taurine / Q000031:analogs & derivatives / Q000494:pharmacology*; D013829:Thiadiazines / Q000494:pharmacology*; D014162:Transfection
D000590:Amino Acid Chloromethyl Ketones; D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D061945:Caspase Inhibitors; D003574:Cytochrome c Group; D013829:Thiadiazines; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; D013654:Taurine; C012566:taurolidine; C505407:CASP3 protein, human; C505409:Casp3 protein, mouse; D053148:Caspase 3