DNA probes on beads arrayed in a capillary, 'Bead-array', exhibited high hybridization performance.
A DNA analysis platform called 'Bead-array' is presented and its features when used in hybridization detection are shown. In 'Bead-array', beads of 100- micro m diameter are lined in a determined order in a capillary. Each bead is conjugated with DNA probes, and can be identified by its order in the capillary. This probe array is easily produced by just arraying beads conjugated with probes into the capillary in a fixed order. The hybridization is also easily completed by introducing samples (1-300 micro l) into the capillary with reciprocal flow. For hybridization detection, as little as 1 amol of fluorescent-labeled oligo DNA was detected. The hybridization reaction was completed in 1 min irrespective of the amount of target DNA. When the number of target molecules was smaller than that of probe molecules on the bead, 10 fmol, almost all targets were captured on the bead. 'Bead-array' enables reliable and reproducible measurement of the target quantity. This rapid and sensitive platform seems very promising for various genetic testing tasks.
Kohara
Yoshinobu
Y
Central Research Laboratory, Hitachi Ltd, 1-280 Higashi-Koigakubo, Kokubunji, Tokyo 185-8601, Japan. y-kohara@crl.hitachi.co.jp
Noda
Hideyuki
H
Okano
Kazunori
K
Kambara
Hideki
H
D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes / Q000235:genetics / Q000378:metabolism*; D005453:Fluorescence; D005898:Glass; D007700:Kinetics; D008856:Microscopy, Fluorescence; D008863:Microspheres; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors; D014966:Xanthenes
D015342:DNA Probes; D014966:Xanthenes; C034657:Texas red; D004247:DNA