Influences of Helicobacter pylori on gastric angiogenesis and ulcer healing in mice.
OBJECTIVE
Helicobacter pylori infection is associated with peptic ulcers; however, it is unclear whether the bacterium delays ulcer healing. We investigated the influence of H. pylori on ulcer healing in mice. We also examined the influence of H. pylori infection on angiogenesis.


METHODS
An acetic acid ulcer was made in male BALB/c mice. Three days later (day 0), the animals were inoculated with H. pylori SS1 strain. The healing process of the ulcer was examined macroscopically and microscopically on days 0, 6 and 9. The index of angiogenesis was also determined using carmine dye injection.


RESULTS
On day 0, angiogenesis began at the ulcer margin while the mucosal epithelia had not yet regenerated. On days 6 and 9, angiogenesis and epithelial regeneration developed and ulcer size reduced. These phenomena were significantly suppressed in mice infected with H. pylori.


CONCLUSIONS
Helicobacter pylori infection significantly suppressed angiogenesis and delayed ulcer healing. These results indicate that H. pylori plays an important role in ulcer healing.
Gunawan
Edhi
E
Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Japan.
Tsuji
Shingo
S
Tsujii
Masahiko
M
Kimura
Arata
A
Sun
Wei-Hao
WH
Sawaoka
Hitoshi
H
Sasayama
Yoshiaki
Y
Sasaki
Yutaka
Y
Kawano
Sunao
S
Hori
Masatsugu
M
D019342:Acetic Acid / Q000633:toxicity; D000818:Animals; D005753:Gastric Mucosa / Q000098:blood supply*; D016481:Helicobacter Infections / Q000382:microbiology / Q000503:physiopathology*; D016480:Helicobacter pylori / Q000502:physiology*; D007202:Indicators and Reagents / Q000633:toxicity; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D018919:Neovascularization, Physiologic; D010437:Peptic Ulcer / Q000139:chemically induced / Q000382:microbiology / Q000503:physiopathology*; D014945:Wound Healing / Q000502:physiology*
D007202:Indicators and Reagents; D019342:Acetic Acid