title: Combined expression of S-VSPalpha in two different organelles enhances its accumulation and total lysine production in leaves of transgenic tobacco plants.
abstract: Soybean vegetative storage proteins (S-VSPs) accumulate to high levels in vacuoles of both wild types and heterologous plants. Here it is shown that directing S-VSPalpha to two different organelles-chloroplasts and vacuoles-in a single transgenic plant significantly increased its accumulation. Accumulation of S-VSPalpha in heterologous plants correlated with total soluble lysine. Using this approach with essential amino-acid-rich transgene proteins may lead to a breakthrough in improving plant nutritional quality.
authors.affiliation: Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan 50250, Israel.
authors.forename: Dana
authors.initials: D
authors.lastname: Guenoune
authors.forename: Rachel
authors.initials: R
authors.lastname: Amir
authors.forename: Hanna
authors.initials: H
authors.lastname: Badani
authors.forename: Shmuel
authors.initials: S
authors.lastname: Wolf
authors.forename: Shmuel
authors.initials: S
authors.lastname: Galili
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008239:Lysine / Q000096:biosynthesis*; D015388:Organelles / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism
chemical_list: D010940:Plant Proteins; C078148:VSPA protein, Glycine max; D000135:Acid Phosphatase; D008239:Lysine