Active-site stereochemical control of oxygen atom transfer reactivity in sulfite oxidase.
A number of both experimental and computational studies have recently been reported for symmetric, six-coordinate dioxomolybdenum(VI) complexes as models of the fully oxidized form of the molybdopterin enzyme sulfite oxidase (SO). Such studies have suggested that the two terminal oxo donors in SO are electronically equivalent. However, the consensus structure of the catalytically competent Mo(VI) active site in SO is five-coordinate square pyramidal, possessing two terminal oxo donors, an ene-1,2-dithiolate chelate and a cysteine sulfur donor ligand. Computational studies at the density functional level of theory have been performed on a minimal model of the SO active site, [Mo(VI)O2(S2C2Me2)(SCH3)]-, in C1 symmetry to evaluate the composition of the LUMO, which is the putative electron acceptor orbital in the oxygen atom transfer (OAT) reaction with the sulfite substrate. The LUMO in this model is principally composed of a Mo dxy - ppi* interaction between the Mo and the equatorial oxygen (Oeq), while the axial oxygen (Oax) possesses no contribution to this orbital. In fact, the LUMO+1 orbital which possesses a substantial amount of Oax character lies nearly 1 eV higher in energy than the LUMO. It has also been suggested that changes in the Oax-Mo-Sthiolate-C torsion angle during the course of enzyme catalysis may aid in selection of Oeq for OAT. Calculations were performed in which this torsion angle was varied by 20 degrees through 360 degrees . These calculations demonstrate that the Mo dxy -Oeq ppi* interaction, and therefore the Oeq atom character, always dominates the LUMO. The results presented here suggest that oxygen atom selection and activation are a direct function of the low-symmetry structure of the oxidized SO active site and provide a role for the ene-1,2-dithiolate in promoting OAT reactivity through its kinetic trans effect on the equatorial oxo donor.
Peariso
Katrina
K
Department of Chemistry at The University of New Mexico, Albuquerque, New Mexico 87131-1096, USA.
McNaughton
Rebecca L
RL
Kirk
Martin L
ML
D001665:Binding Sites; D006801:Humans; D007202:Indicators and Reagents; D008958:Models, Molecular; D008982:Molybdenum / Q000737:chemistry*; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry*; D011487:Protein Conformation
D007202:Indicators and Reagents; D008982:Molybdenum; D050862:Oxidoreductases Acting on Sulfur Group Donors; D010100:Oxygen