title: Rolling properties of rGPIbalpha-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions.
abstract: The recombinant fragment of the platelet membrane glycoprotein, rGPIbalpha, was conjugated to phospholipid vesicles with the average diameter of ca. 1 microm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIbalpha-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIbalpha-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.
authors.affiliation: Artificial Blood Project, Advanced Research Institute for Science and Engineering, Waseda University, Tokyo 169-8555, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Takeoka
authors.forename: Yuji
authors.initials: Y
authors.lastname: Teramura
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Okamura
authors.forename: Eishun
authors.initials: E
authors.lastname: Tsuchida
authors.forename: Makoto
authors.initials: M
authors.lastname: Handa
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ikeda
mesh_terms: D001792:Blood Platelets / Q000502:physiology; D002465:Cell Movement; D005454:Fluorescence Polarization; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry; D008954:Models, Biological; D009038:Motion; D010743:Phospholipids / Q000737:chemistry*; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013314:Stress, Mechanical; D013696:Temperature; D014841:von Willebrand Factor / Q000378:metabolism*
chemical_list: D008081:Liposomes; D010743:Phospholipids; D019038:Platelet Glycoprotein GPIb-IX Complex; D011994:Recombinant Proteins; D014841:von Willebrand Factor