Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray.
Full-length cDNAs are essential for functional analysis of plant genes in the post-sequencing era of the Arabidopsis genome. Recently, cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles. We have prepared a full-length cDNA microarray containing approximately 7000 independent, full-length cDNA groups to analyse the expression profiles of genes under drought, cold (low temperature) and high-salinity stress conditions over time. The transcripts of 53, 277 and 194 genes increased after cold, drought and high-salinity treatments, respectively, more than fivefold compared with the control genes. We also identified many highly drought-, cold- or high-salinity- stress-inducible genes. However, we observed strong relationships in the expression of these stress-responsive genes based on Venn diagram analysis, and found 22 stress-inducible genes that responded to all three stresses. Several gene groups showing different expression profiles were identified by analysis of their expression patterns during stress-responsive gene induction. The cold-inducible genes were classified into at least two gene groups from their expression profiles. DREB1A was included in a group whose expression peaked at 2 h after cold treatment. Among the drought, cold or high-salinity stress-inducible genes identified, we found 40 transcription factor genes (corresponding to approximately 11% of all stress-inducible genes identified), suggesting that various transcriptional regulatory mechanisms function in the drought, cold or high-salinity stress signal transduction pathways.
Seki
Motoaki
M
Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan.
Narusaka
Mari
M
Ishida
Junko
J
Nanjo
Tokihiko
T
Fujita
Miki
M
Oono
Youko
Y
Kamiya
Asako
A
Nakajima
Maiko
M
Enju
Akiko
A
Sakurai
Tetsuya
T
Satou
Masakazu
M
Akiyama
Kenji
K
Taji
Teruaki
T
Yamaguchi-Shinozaki
Kazuko
K
Carninci
Piero
P
Kawai
Jun
J
Hayashizaki
Yoshihide
Y
Shinozaki
Kazuo
K
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D003080:Cold Temperature; D003890:Desiccation; D004190:Disasters; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000139:chemically induced / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D018749:RNA, Plant / Q000032:analysis / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors; D015533:Transcriptional Activation
D018749:RNA, Plant; D012965:Sodium Chloride