New approach to the adoptive immunotherapy of Walker-256 carcinosarcoma with activated macrophages combined with photodynamic therapy.
Experiments were performed on five batches of Wistar inbred rats with Walker-256 carcinoma receiving sole (PDT, MAK) or combined therapy (PDT + MAK-A; PDT + MAK-B); the control batch (HBSS) consisted of animals with untreated tumors. The results were as follows: a) the sole treatment (PDT, MAK) gave survival rates between 37.7 and 47.5%, b) the "combined" therapy in five doses increased significantly (70.8%) the survival rate of tumor bearing rate as well as the rate of complete regression (82.1%). The cell-mediated immunity test and histopathological as well as the electron microscopy observations were in full agreement with the results above. Summing up, these results demonstrate that "combined" photodynamic therapy with intra- and peritumoral MAK infusion stimulated cell-mediated antitumoral activity, increased survival rates and reduces incidence of Walker-256 carcinoma in rat model.
Dima
V F
VF
Cantacuzino Institute, Bucharest, Romania.
Ionescu
M D
MD
Balotescu
C
C
Dima
S V
SV
Vasiliu
V
V
Lacky
D
D
D000818:Animals; D002279:Carcinoma 256, Walker / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D003131:Combined Modality Therapy; D003602:Cytotoxicity, Immunologic; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008262:Macrophage Activation; D008854:Microscopy, Electron; D010778:Photochemotherapy; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000276:immunology