Crystal structure of recombinant human interleukin-22.
Interleukin-22 (IL-10-related T cell-derived inducible factor/IL-TIF/IL-22) is a novel cytokine belonging to the IL-10 family. Recombinant human IL-22 (hIL-22) was found to activate the signal transducers and activators of transcription factors 1 and 3 as well as acute phase reactants in several hepatoma cell lines, suggesting its involvement in the inflammatory response. The crystallographic structure of recombinant hIL-22 has been solved at 2.0 A resolution using the SIRAS method. Contrary to IL-10, the hIL-22 dimer does not present an interpenetration of the secondary-structure elements belonging to the two distinct polypeptide chains but results from interface interactions between monomers. Structural differences between these two cytokines, revealed by the crystallographic studies, clearly indicate that, while a homodimer of IL-10 is required for signaling, hIL-22 most probably interacts with its receptor as a monomer.
Nagem
Ronaldo Alves Pinto
RA
Laboratório Nacional de Luz Síncrotron, São Paulo, Brazil.
Colau
Didier
D
Dumoutier
Laure
L
Renauld
Jean-Christophe
JC
Ogata
Craig
C
Polikarpov
Igor
I
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018360:Crystallography, X-Ray; D019281:Dimerization; D004220:Disulfides / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000737:chemistry / Q000235:genetics; D016753:Interleukin-10 / Q000737:chemistry / Q000235:genetics; D007378:Interleukins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D018123:Receptors, Interleukin / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D000097043:Interleukin-22
D004220:Disulfides; D007371:Interferon-gamma; D016753:Interleukin-10; D007378:Interleukins; D018123:Receptors, Interleukin; D011994:Recombinant Proteins; D000097043:Interleukin-22