Control of chromosome replication in caulobacter crescentus.
Caulobacter crescentus permits detailed analysis of chromosome replication control during a developmental cell cycle. Its chromosome replication origin (Cori) may be prototypical of the large and diverse class of alpha-proteobacteria. Cori has features that both affiliate and distinguish it from the Escherichia coli chromosome replication origin. For example, requirements for DnaA protein and RNA transcription affiliate both origins. However, Cori is distinguished by several features, and especially by five binding sites for the CtrA response regulator protein. To selectively repress and limit chromosome replication, CtrA receives both protein degradation and protein phosphorylation signals. The signal mediators, proteases, response regulators, and kinases, as well as Cori DNA and the replisome, all show distinct patterns of temporal and spatial organization during cell cycle progression. Future studies should integrate our knowledge of biochemical activities at Cori with our emerging understanding of cytological dynamics in C. crescentus and other bacteria.
Marczynski
Gregory T
GT
Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada H3A 2B4. gmarczynski@microimm.mcgill.ca
Shapiro
Lucy
L
D001426:Bacterial Proteins; D016935:Caulobacter crescentus / Q000235:genetics* / Q000378:metabolism; D020090:Chromosome Segregation; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D019175:DNA Methylation; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D018741:Replication Origin / Q000235:genetics / Q000502:physiology; D012093:Replicon; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D001426:Bacterial Proteins; C097706:CtrA protein, Caulobacter; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C098075:DNA modification methylase CcrM; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)