title: Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates.
abstract: The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide-dye conjugates in a variety of living cells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide-dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands.
authors.affiliation: Division of Chemistry and Chemical Engineering and Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Belitsky
authors.forename: Stephanie J
authors.initials: SJ
authors.lastname: Leslie
authors.forename: Paramjit S
authors.initials: PS
authors.lastname: Arora
authors.forename: Terry A
authors.initials: TA
authors.lastname: Beerman
authors.forename: Peter B
authors.initials: PB
authors.lastname: Dervan
mesh_terms: D001896:Boron Compounds / Q000493:pharmacokinetics; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D006801:Humans; D007093:Imidazoles / Q000493:pharmacokinetics*; D018613:Microscopy, Confocal; D009757:Nylons / Q000493:pharmacokinetics*; D011758:Pyrroles / Q000493:pharmacokinetics*; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D007093:Imidazoles; D009757:Nylons; D011758:Pyrroles; C096654:N-methylpyrrole; C018100:1-methylimidazole