title: Deficiency of epidermal protein-bound omega-hydroxyceramides in atopic dermatitis.
abstract: Atopic dermatitis is a common skin disease of unknown etiology with an impaired permeability barrier function. To learn more about the molecular pathology in lesional skin, we analyzed levels of free extractable as well as protein-bound barrier lipids in the epidermis of atopic dermatitis subjects. The amount of protein-bound omega-hydroxyceramides in healthy epidermis comprised 46-53 wt% of total protein-bound lipids, whereas this percentage was decreased to 23-28 wt% in nonlesional areas and even down to 10-25 wt% in affected atopic skin areas of the subjects. Furthermore, the partial amount of free extractable very long chain fatty acids with more than 24 carbon atoms was reduced in affected regions down to 25 wt% and in nonlesional regions of the atopic dermatitis subjects down to 40 wt% compared to healthy controls. This "hydrocarbon chain length deficiency" regarding the barrier lipids in atopic skin was supported by metabolic labeling studies with [14C]-serine in cultured epidermis. The biosynthesis of free glucosylceramides and free ceramides was remarkably decreased in affected skin areas of the atopic subjects compared to healthy control subjects. Especially affected were the de novo syntheses of ceramide 4 (i.e., ceramide EOH, consisting of a very long chain N-acyl omega-hydroxy fatty acid esterified with linoleic acid and 6-hydroxysphingosine as sphingoid base) and ceramide 3 (ceramide NP, consisting of a nonhydroxy N-acyl fatty acid and phytosphingosine). In conclusion, this study revealed that the lesional epidermis in atopic dermatitis has considerable deficiencies within main barrier lipid components, which may contribute to the severely damaged permeability barrier.
authors.affiliation: Kekulé-Institut für Organische Chemie und Biochemie, Universitaet Bonn, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Macheleidt
authors.forename: Hans Wilhelm
authors.initials: HW
authors.lastname: Kaiser
authors.forename: Konrad
authors.initials: K
authors.lastname: Sandhoff
mesh_terms: D000293:Adolescent; D000328:Adult; D001706:Biopsy; D003876:Dermatitis, Atopic / Q000378:metabolism* / Q000473:pathology; D004817:Epidermis / Q000378:metabolism* / Q000473:pathology; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis / Q000378:metabolism; D005260:Female; D006028:Glycosphingolipids / Q000096:biosynthesis / Q000172:deficiency*; D006801:Humans; D006900:Hydroxylation; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000378:metabolism
chemical_list: D005230:Fatty Acids, Nonesterified; D006028:Glycosphingolipids; D011506:Proteins; C018480:ceramide 3