title: Hyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cells.
abstract: One of the biological effects of hyperbaric oxygen (HBO) therapy in enhancing ischemia-related wound healing is the induction of angiogenesis. To elucidate the mechanism(s) underlying the HBO-induced angiogenesis, we studied the expression of several angiogenesis-related genes in human umbilical vein endothelial cells exposed to HBO. Western blot analyses showed that HBO enhanced the expression of angiopoietin-2 (Ang2) with no effect on the expression of Tie2, angiopoietin-1, and VEGF. The induction of Ang2 was further confirmed by immunohistochemistry, quantitative PCR, and Northern blot analyses. Inhibition of endothelial nitric oxide synthase blocked the HBO-induced Ang2 expression, but failed to block hypoxia-induced Ang2 expression. These data indicated that HBO-induced Ang2 expression may be through transcriptional stimulation, and requires the nitric oxide signaling pathway, which may play an important role in HBO-induced angiogenesis.
authors.affiliation: The Central Laboratory, Shin Kong Wu Ho-Su Memorial Hospital, 95 Wen-Chang Road, Shih-Lin District, Taipei, Taiwan, ROC.
authors.forename: Shankung
authors.initials: S
authors.lastname: Lin
authors.forename: Kou-Gi
authors.initials: KG
authors.lastname: Shyu
authors.forename: Chun-Chung
authors.initials: CC
authors.lastname: Lee
authors.forename: Bao-Wei
authors.initials: BW
authors.lastname: Wang
authors.forename: Chih-Chuan
authors.initials: CC
authors.lastname: Chang
authors.forename: Ya-Chen
authors.initials: YC
authors.lastname: Liu
authors.forename: Fan-Yen
authors.initials: FY
authors.lastname: Huang
authors.forename: Hang
authors.initials: H
authors.lastname: Chang
mesh_terms: D042702:Angiopoietin-2; D015687:Cell Hypoxia; D002478:Cells, Cultured; D016228:Endothelial Growth Factors / Q000096:biosynthesis / Q000235:genetics; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006931:Hyperbaric Oxygenation; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III; D010100:Oxygen / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D015533:Transcriptional Activation; D014471:Umbilical Veins / Q000166:cytology*; D015854:Up-Regulation
chemical_list: D042702:Angiopoietin-2; D016228:Endothelial Growth Factors; D011506:Proteins; D012333:RNA, Messenger; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; D010100:Oxygen