Interaction of NahR, a LysR-type transcriptional regulator, with the alpha subunit of RNA polymerase in the naphthalene degrading bacterium, Pseudomonas putida NCIB 9816-4.
NahR, a LysR-type transcriptional regulator, is required for expression of naphthalene catabolic operons. However, detailed mechanisms of transcriptional activation by NahR are poorly understood. Many transcriptional activators make direct contact with RNA polymerase (RNAP) to initiate transcription. We investigated the hypothesis that direct contact between NahR and the alpha subunit of RNAP (alphaRNAP) may be involved in expression of the naphthalene catabolic operons in Pseudomonas putida NCIB 9816-4. Interactions between the NahR and alphaRNAP in P. putida NCIB 9816-4 were analyzed using the yeast two-hybrid system. The results obtained indicate that protein-protein interactions occur between alphaRNAP and the NahR. Gene activation by NahR is consistent with the general transcriptional mechanism of class I transcription factors, which function by contacting alphaRNAP.
Park
Woojun
W
Department of Microbiology, Cornell University, Ithaca, NY 14853-8101, USA.
Jeon
Che Ok
CO
Madsen
Eugene L
EL
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D001673:Biodegradation, Environmental; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000378:metabolism*; D011485:Protein Binding; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000378:metabolism
D001426:Bacterial Proteins; C063173:NahR protein, Bacteria; D009281:Naphthalenes; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases; C113275:RNA polymerase alpha subunit; D001616:beta-Galactosidase