Stem cell properties of human dental pulp stem cells.
In this study, we characterized the self-renewal capability, multi-lineage differentiation capacity, and clonogenic efficiency of human dental pulp stem cells (DPSCs). DPSCs were capable of forming ectopic dentin and associated pulp tissue in vivo. Stromal-like cells were reestablished in culture from primary DPSC transplants and re-transplanted into immunocompromised mice to generate a dentin-pulp-like tissue, demonstrating their self-renewal capability. DPSCs were also found to be capable of differentiating into adipocytes and neural-like cells. The odontogenic potential of 12 individual single-colony-derived DPSC strains was determined. Two-thirds of the single-colony-derived DPSC strains generated abundant ectopic dentin in vivo, while only a limited amount of dentin was detected in the remaining one-third. These results indicate that single-colony-derived DPSC strains differ from each other with respect to their rate of odontogenesis. Taken together, these results demonstrate that DPSCs possess stem-cell-like qualities, including self-renewal capability and multi-lineage differentiation.
Gronthos
S
S
Craniofacial and Skeletal Diseases Branch, Building 30, Room 228, NIDCR, NIH, Bethesda, MD 20892, USA
Brahim
J
J
Li
W
W
Fisher
L W
LW
Cherman
N
N
Boyde
A
A
DenBesten
P
P
Robey
P Gehron
PG
Shi
S
S
D000135:Acid Phosphatase / Q000032:analysis; D017667:Adipocytes / Q000166:cytology; D000328:Adult; D000818:Animals; D016026:Bone Marrow Transplantation; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D002999:Clone Cells / Q000502:physiology; D003094:Collagen / Q000032:analysis; D003782:Dental Pulp / Q000166:cytology*; D003804:Dentin / Q000166:cytology; D003810:Dentinogenesis / Q000502:physiology; D062109:Dermatologic Surgical Procedures; D016326:Extracellular Matrix Proteins; D005434:Flow Cytometry; D006801:Humans; D016867:Immunocompromised Host; D007527:Isoenzymes / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology; D009804:Odontoblasts / Q000166:cytology; D009805:Odontogenesis / Q000502:physiology; D010750:Phosphoproteins; D011498:Protein Precursors; D012038:Regeneration / Q000502:physiology; D012795:Sialoglycoproteins / Q000032:analysis; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology*; D017154:Stromal Cells / Q000637:transplantation; D000071681:Tartrate-Resistant Acid Phosphatase; D000099311:Dentin Sialophosphoprotein
D000135:Acid Phosphatase; D003094:Collagen; D016326:Extracellular Matrix Proteins; D007527:Isoenzymes; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D000071681:Tartrate-Resistant Acid Phosphatase; D000099311:Dentin Sialophosphoprotein