title: Alcohol and nicotine administration inhibits serotonin synthesis and tryptophan hydroxylase expression in dorsal and median raphe of young rats.
abstract: Serotonin (5-hydroxytryptamine, 5-HT) has been implicated in the pathophysiology of various neuropsychiatric disorders. In the present study, the effects of alcohol and nicotine on the synthesis of 5-HT and the expression of tryptophan hydroxylase (TPH), the rate-limiting enzyme of 5-HT synthesis, in the dorsal and median raphe of young rats were investigated via immunohistochemistry. The numbers of the 5-HT-positive and TPH-positive cells were reduced by alcohol and nicotine treatment in a dose-dependent manner. Based on the results, it can be suggested that the pathogenesis of alcohol- and nicotine-induced neuropsychological disorders involves alcohol- and nicotine-induced suppression of 5-HT synthesis and TPH expression in raphe, and that this may be of particular relevance in the consumption of alcohol and nicotine during adolescence.
authors.affiliation: Department of Physiology, College of Medicine, Kyung Hee University, #1 Hoigi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
authors.forename: Mi-Hyeon
authors.initials: MH
authors.lastname: Jang
authors.forename: Min-Chul
authors.initials: MC
authors.lastname: Shin
authors.forename: Taeck-Hyun
authors.initials: TH
authors.lastname: Lee
authors.forename: Young-Pyo
authors.initials: YP
authors.lastname: Kim
authors.forename: Sae-Bin
authors.initials: SB
authors.lastname: Jung
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Shin
authors.forename: Hong
authors.initials: H
authors.lastname: Kim
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Ee-Hwa
authors.initials: EH
authors.lastname: Kim
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000375:Aging / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000008:administration & dosage*; D000431:Ethanol / Q000008:administration & dosage*; D007274:Injections, Intraperitoneal; D008297:Male; D009538:Nicotine / Q000008:administration & dosage*; D011903:Raphe Nuclei / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000096:biosynthesis*; D012702:Serotonin Antagonists / Q000008:administration & dosage*; D014365:Tryptophan Hydroxylase / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*
chemical_list: D004791:Enzyme Inhibitors; D012702:Serotonin Antagonists; D012701:Serotonin; D000431:Ethanol; D009538:Nicotine; D014365:Tryptophan Hydroxylase