title: A Novel Reaction Media for Horseradish Peroxidase (HRP) Catalysis in Organic Solvents.
abstract: It is important to select favorable reaction media for use of enzymes in organic synthesis. In the presence of PEG with a certain molecular weight, toluene was able to dissolve horseradish peroxidase and the latter maintained a high activity. The HRP reaction conditions, such as ratio of PEG/toluene, water content, pH, and substrate concentration were investigated. It was found that the activity of HRP increased when PEG concentration was low and water content was high. In such a system, the enzyme functioned best at pH 7.0,the concentration of H(2)O(2) and guaiacol being 20 mmol/L and 0.1 mol/L respectively. Under the same condition, no activity was detected in the PEG / ethanol and PEG / dioxane systems. However, in PEG / toluene system, HRP maintained a higher activity and the activity was ten-fold more than that in toluene alone.
authors.affiliation: Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. zhongyi@sunm.shcnc.ac.cn
authors.forename: Tai-Jiao
authors.initials: TJ
authors.lastname: Jiang
authors.forename: Xin-Song
authors.initials: XS
authors.lastname: Ji
authors.forename: Zhong-Yi
authors.initials: ZY
authors.lastname: Yuan