title: Detection of enteroaggregative Escherichia coli with formalin-preserved HEp-2 cells.
abstract: Formalin-stored HEp-2 cells were used to assay Escherichia coli for adherence. Cells refrigerated in formalin for up to 28 days and used in a wet assay format demonstrated an assay sensitivity ranging from 94 to 98% to detect enteroaggregative E. coli (EAEC). HEp-2 cells first fixed and stored with formalin and then stored dry in ambient conditions for 6 weeks demonstrated an assay sensitivity of 92% to detect EAEC. Using formalin-fixed HEp-2 cells will improve the efficiency of EAEC identification.
authors.affiliation: Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Mohamad S
authors.initials: MS
authors.lastname: Miqdady
authors.forename: Zhi-Dong
authors.initials: ZD
authors.lastname: Jiang
authors.forename: James P
authors.initials: JP
authors.lastname: Nataro
authors.forename: Herbert L
authors.initials: HL
authors.lastname: DuPont
mesh_terms: D001422:Bacterial Adhesion; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000502:physiology; D004927:Escherichia coli Infections / Q000382:microbiology; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D005404:Fixatives; D005557:Formaldehyde