title: Effects of topology and excluded volume on protein denatured state conformational properties.
abstract: The conformational constraints on protein denatured states are of prime importance in modulating early events in protein folding. Although structural studies have demonstrated residual structure in protein denatured states, much remains poorly understood with regard to the conformational properties of this state. Here, we investigate topological effects on loop formation probabilities in denatured iso-1-cytochrome c by comparing histidine-heme binding affinities for histidines on the N- versus the C-terminal side of the heme. For histidines N-terminal to the heme (preceding cysteine 14), the polypeptide emerges from the edge of the heme and must simply fold over to bind to the heme. For histidines C-terminal to the heme (following histidine 18), the polypeptide emerges from the back side of the heme and must wrap around the heme for the histidine to bind to the heme. Thus, the steric constraints on this wrap-around topology are expected to be much more demanding than for the heme-edge topology of the N-terminal histidines. Evaluation of loop formation probabilities in 3 M guanidine hydrochloride, conditions that fully denature the variants studied, demonstrates that N-terminal histidine-heme loop formation is 10-25-fold more favorable than C-terminal histidine-heme loop formation, for similar loop sizes. A two-dimensional square lattice model indicates that excluded volume is important in this topological preference. These data provide direct evidence that denatured state topology affects contact probability, and thus probable folding pathways, in a disordered protein.
authors.affiliation: Department of Chemistry and Biochemistry, University of Denver, 2190 East Iliff Avenue, Denver, CO 80208, USA.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Smith
authors.forename: Natasa
authors.initials: N
authors.lastname: Mateljevic
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Bowler
mesh_terms: D001483:Base Sequence; D003574:Cytochrome c Group / Q000737:chemistry; D045304:Cytochromes c; D009841:Oligonucleotides; D011487:Protein Conformation; D011489:Protein Denaturation; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D013816:Thermodynamics
chemical_list: C014059:CYC1 protein, S cerevisiae; D003574:Cytochrome c Group; D009841:Oligonucleotides; D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c