Differentiation of adult bone marrow stem cells into neuroprogenitor cells in vitro.
We found the expression of neurofilament was very low in undifferentiated human adult bone marrow mesenchymal stem cells (hMSCs), but its expression could be significantly induced after treatment with combination of growth factors. Approximately 16% of hMSCs differentiated into cells expressing neurofilament after treatment with a combination of FGF and RA, retinoic acid. We also examined the effect of five different cell culture substrates on the expression of neurofilament. One specific combination that was particularly effective in provoking pre-neuronal differentiation was culturing hMSCs on fibronectin-coated dishes and stimulating them with FGF and RA; 40% of cells expressed neurofilament. These results suggest that growth factors and substrates, in combination, can effectively initiate differentiation of hMSCs along a neuronal pathway.
Kim
Byoung J
BJ
Department of Medicine, Sungkyunkwan University, School of Medicine, Seoul, Korea 135-710.
Seo
Jeong H
JH
Bubien
James K
JK
Oh
Young S
YS
D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D019070:Cell Lineage / Q000187:drug effects / Q000502:physiology; D048429:Cell Size / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002493:Central Nervous System Diseases / Q000628:therapy*; D005576:Colforsin / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D004338:Drug Combinations; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D005353:Fibronectins / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D008297:Male; D008648:Mesoderm / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016900:Neurofilament Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013873:Thionucleotides / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D003470:Culture Media; D004338:Drug Combinations; D005353:Fibronectins; D006133:Growth Substances; D016900:Neurofilament Proteins; D012333:RNA, Messenger; D013873:Thionucleotides; D005576:Colforsin; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D014212:Tretinoin; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D000242:Cyclic AMP