title: Genotyping of hepatitis E virus from Vietnam.
abstract: To identify the genotype of Vietnamese isolates of human hepatitis E virus (HEV), phylogenetic analysis was performed for the open reading frame (ORF) 1 and ORF2 nucleotide sequences of the viral genome. HEV was detected by RT-PCR in 9 out of 141 sera collected from patients with a diagnosis of acute sporadic hepatitis in Hanoi, Vietnam. All of them had sequences related most closely to genotype 4. In addition, the Vietnamese isolate had a single nucleotide insertion in the ORF 3 region, a characteristic reported for genotype 4 with the possible change of initiation of ORF 3 and ORF 2.
authors.affiliation: Department of Respiratory Diseases, Research Institute, International Medical Center of Japan, Tokyo, Japan. minakoh@ri.imcj.go.jp
authors.forename: Minako
authors.initials: M
authors.lastname: Hijikata
authors.forename: Shigeki
authors.initials: S
authors.lastname: Hayashi
authors.forename: Ngoc Thi
authors.initials: NT
authors.lastname: Trinh
authors.forename: Le Dang
authors.initials: le D
authors.lastname: Ha
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohara
authors.forename: Yohko K
authors.initials: YK
authors.lastname: Shimizu
authors.forename: Naoto
authors.initials: N
authors.lastname: Keicho
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshikura
mesh_terms: D001483:Base Sequence; D005260:Female; D005838:Genotype; D016751:Hepatitis E / Q000453:epidemiology / Q000821:virology; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D014176:Protein Biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017421:Sequence Analysis; D014744:Vietnam