title: Cell proliferation and chromosomal changes in human ameloblastoma.
abstract: Cell proliferation and chromosomal imbalances, important parameters in relation to tumor progression, were studied in ameloblastoma (n=20), a benign odontogenic tumor of locally recurrent nature. Immunocytochemical staining with MIB-1 antibody and comparative genomic hybridization (CGH) were performed on formalin-fixed paraffin-embedded ameloblastomas. The mean follow-up time was 12.4 years. An MIB-1-index was formed by counting 5000 tumor-cell nuclei in 10-15 randomly chosen high-power fields and calculating percentages of positively stained cells. CGH involved hybridization of FITC-dUTP-labeled tumor DNA with Texas-red-labeled normal DNA. Images were digitally analyzed. The MIB-1-index (range 0-2.51) was low for all tumors. No statistically significant correlation between MIB-1 index and tendency to recurrence was found. Chromosomal aberrations were detected in 2 of 17 cases. The results suggest that formation of an MIB-1 index is not helpful in assessing future clinical behavior of an ameloblastoma and that chromosomal imbalances are uncommon.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, FIN-20520, Turku, Finland.
authors.forename: Karoliina
authors.initials: K
authors.lastname: Jääskeläinen
authors.forename: Kowan J
authors.initials: KJ
authors.lastname: Jee
authors.forename: Ilmo
authors.initials: I
authors.lastname: Leivo
authors.forename: Irma
authors.initials: I
authors.lastname: Saloniemi
authors.forename: Sakari
authors.initials: S
authors.lastname: Knuutila
authors.forename: Kristiina
authors.initials: K
authors.lastname: Heikinheimo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000564:Ameloblastoma / Q000235:genetics / Q000473:pathology*; D034961:Antigens, Nuclear; D002455:Cell Division; D002869:Chromosome Aberrations; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007573:Jaw Neoplasms / Q000235:genetics / Q000473:pathology*; D019394:Ki-67 Antigen; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000378:metabolism; D014070:Tooth / Q000254:growth & development / Q000378:metabolism
chemical_list: D034961:Antigens, Nuclear; D019394:Ki-67 Antigen; D009687:Nuclear Proteins