Hamster DEC-205, its primary structure, tissue and cellular distribution.
DEC-205, a putative antigen uptake receptor, belongs to a family of transmembrane C-type lectins. This molecule is known to be one of the most authentic markers for the lineage of dendritic cells. In the present study, we determined the primary structure, tissue distribution and cellular localization of hamster DEC-205. The multi-domain structure of mouse and human DEC-205 was completely conserved in hamster with the overall identity of approximately 80%. DEC-205 transcripts were detected in the thymus and bone marrow cells cultured in the presence of mouse granulocyte macrophage colony-stimulating factor and interleukin-4 in which the DEC-205 expression was up-regulated in the course of cultures. Hamster DEC-205 was mainly detected on cell membrane and shown to mediate the uptake of flourescein isothiocyanate-conjugated ovalbumin. DEC-205 is a highly conserved molecule across the species suggesting its fundamental role in the immune system.
Maruyama
Kouji
K
Growth Factor Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo, Japan.
Akiyama
Yasuto
Y
Cheng
Jinyan
J
Nara-Ashizawa
Noriko
N
Hojo
Takashi
T
Sasaki
Kazuki
K
Yamaguchi
Ken
K
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D001854:Bone Marrow Cells / Q000378:metabolism; D002478:Cells, Cultured; D006224:Cricetinae; D004705:Endocytosis; D005260:Female; D006801:Humans; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins / Q000032:analysis / Q000737:chemistry* / Q000502:physiology; D008647:Mesocricetus; D051379:Mice; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000032:analysis / Q000737:chemistry* / Q000502:physiology; D017385:Sequence Homology; D014162:Transfection
D015703:Antigens, CD; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D015778:Minor Histocompatibility Antigens; D012333:RNA, Messenger; D011956:Receptors, Cell Surface