title: Adequate evaluation of HSL mass and activity in rat adipose tissue in fasting and aging-related obesity.
abstract: Adipose tissue is a unique tissue because its mass is readily changed by altering nutritional conditions. Therefore the activity and content of enzyme in the adipose tissue is significantly differed according to the way of their presentation: per g tissue, per whole tissue, or per cell number. In the present study, the effects of the ways of expressing the hormone sensitive lipase (HSL) activity and content were studied in rat by decreasing or increasing adipose tissue. Fasting caused a progressive decline in body weight and in the weight of the epididymal fat pad. When the HSL content was expressed per g of adipose tissue, the lipase activity and immunoreactive HSL protein content in fasting rats were higher than those in fed rats. On the other hand, when they were expressed as per fat pad, the lipase activity and immunoreactive HSL protein in fasting rats were lower than those in fed rats. The opposite results were observed in obesity. When the HSL content was expressed per g of adipose tissue, the lipase activity and immunoreactive HSL protein in obese rats were lower than in control rats. However, when the HSL content was expressed per fat pad, the lipase activity and immunoreactive HSL protein in the obese rats were higher than in the control rats. Therefore we must pay careful attention to the way of presentation of adipose tissue enzyme contents.
authors.affiliation: Central Research Laboratory, School of Medicine, Ehime University, Shigenobu, Onsen-gun, Japan. tsujita@m.ehime-u.ac.jp
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tsujita
authors.forename: Maho
authors.initials: M
authors.lastname: Sumiyoshi
authors.forename: Chie
authors.initials: C
authors.lastname: Morimoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Kameda
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Okuda
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology / Q000201:enzymology*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose; D001835:Body Weight; D004822:Epididymis; D005215:Fasting / Q000378:metabolism*; D005227:Fatty Acids / Q000097:blood; D008049:Lipase / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000209:etiology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D005227:Fatty Acids; D014280:Triglycerides; D008049:Lipase