HIV type 1 glycoprotein 120 inhibits cardiac myocyte contraction.
Cardiomyopathy is a common, life-threatening, but poorly understood complication of HIV infection. The purpose of the present study is to study the effects of an HIV surface envelope protein, glycoprotein 120 (gp120), on cell contraction and L-type Ca(2+) current in rabbit ventricular myocytes. Rabbit ventricular cells were isolated by an enzyme dissociation method. Cell contractions were induced by electric field stimulation. Whole cell L-type Ca(2+) channel currents were measured by the whole cell voltage-clamp technique. We found that perfusion with solution containing gp120 (0.1 microg/ml) derived from HIV-1(SF2) significantly inhibited field-stimulated contractions and L-type Ca(2+) current in rabbit ventricular myocytes as compared with perfusion with buffer alone. These results suggest that HIV-1 gp120 may directly contribute to cardiac dysfunction as seen in many HIV patients.
Chen
Fuhua
F
UCLA School of Medicine, 675 C.E. Young Drive South, MRL 3754, Los Angeles, CA 90095-7045, USA. fchen@mednet.ucla.edu
Shannon
Kevin
K
Ding
Shulan
S
Silva
Monica E
ME
Wetzel
Glenn T
GT
Klitzner
Thomas S
TS
Krogstad
Paul
P
D000818:Animals; D002118:Calcium / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000187:drug effects; D004558:Electric Stimulation; D015699:HIV Envelope Protein gp120 / Q000494:pharmacology*; D015497:HIV-1 / Q000737:chemistry*; D009200:Myocardial Contraction / Q000187:drug effects*; D011817:Rabbits; D014981:Xenopus
D020746:Calcium Channels, L-Type; D015699:HIV Envelope Protein gp120; D002118:Calcium