Serologic and molecular detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (human granulocytic ehrlichiosis agent) in Korean patients.
Sera from 491 Korean patients with acute febrile diseases were tested for Ehrlichia chaffeensis and Anaplasma phagocytophila antibodies by indirect immunofluorescence assay (IFA), Western blotting, and TaqMan real-time PCR. Overall, 0.4% of sera reacted with E. chaffeensis, and 1.8% reacted with A. phagocytophila in IFAs. This is the first report of detection of antibodies to A. phagocytophila and E. chaffeensis in Korea and suggests the presence of A. phagocytophila and E. chaffeensis or antigenically similar species.
Heo
Eun-jeong
EJ
Department of Veterinary Internal Medicine and Clinical Pathology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561-756, Korea.
Park
Jin-ho
JH
Koo
Ja-ryong
JR
Park
Man-suk
MS
Park
Mi-yeoun
MY
Dumler
J Stephen
JS
Chae
Joon-seok
JS
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000709:Anaplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000097:blood*; D015153:Blotting, Western; D018469:Ehrlichia chaffeensis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007723:Korea / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism
D000907:Antibodies, Bacterial; D019914:Taq Polymerase