Extended leaf longevity in the ore4-1 mutant of Arabidopsis with a reduced expression of a plastid ribosomal protein gene.
The longevity of plant leaf organs is genetically determined. However, the molecular mechanisms underlying the control of longevity are still largely unknown. Here, we describe a T-DNA-insertional mutation of Arabidopsis thaliana that confers extended leaf longevity. The mutation, termed ore4-1, delays a broad spectrum of age-dependent leaf senescence, but has little effect on leaf senescence artificially induced by darkness, abscisic acid (ABA), methyl jasmonate (MeJA), or ethylene. The T-DNA was inserted within the promoter region of the plastid ribosomal small subunit protein 17 (PRPS17) gene, and this insertion dramatically reduced PRPS17 mRNA expression. In the ore4-1 mutant, the leaf growth rate is decreased, while the maturation timing is similar to that of wild-type. In addition, the activity of the photosystem I (PSI) is significantly reduced in the ore4-1 mutant, as compared to wild-type. Thus, the ore4-1 mutation results in a deficiency in various chloroplast functions, including photosynthesis, which may decrease leaf growth. Our results suggest a possible link between reduced metabolism and extended longevity of the leaf organs in the ore4-1 mutation.
Woo
Hye Ryun
HR
Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Kyungbuk 790-784, Korea.
Goh
Chang-Hyo
CH
Park
Joon-Hyun
JH
Teyssendier de la Serve
Bernard
B
Kim
Jin-Hee
JH
Park
Youn-Il
YI
Nam
Hong Gil
HG
D000375:Aging / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D005808:Genes, Recessive; D005816:Genetic Complementation Test; D008136:Longevity / Q000235:genetics; D016254:Mutagenesis, Insertional; D009154:Mutation; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development*; D018087:Plastids / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*
D029681:Arabidopsis Proteins; D012269:Ribosomal Proteins