title: Cloning and Sequencing of the Gene Encoding NS Protein of Wheat Rosette Stunt Virus.
abstract: The cDNA fragment located downstream of the WRSV N protein gene was obtained from the cDNA library of WRSV by hybridization with a 24 nucleotides sequence fragment of the 3' end of mRNA of the N protein. The cDNA fragment was sequenced and on ORF encoding a 40 kD protein was found. The gene was expressed in E. coli DE3 and was identified by Western blot experiment as the NS gene of WRSV.
authors.affiliation: Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. gongzx@sunm.shcnc.ac.cn
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Shen
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Ye
authors.forename: Zu-Xun
authors.initials: ZX
authors.lastname: Gong