Overexpression of cyclin D1 enhances taxol induced mitotic death in MCF7 cells.
Treatment of MCF7 human breast cancer cells with taxol induces G2/M arrest followed by mitotic death. A moderate overexpression of ectopic cyclin D1 accelerated these G2/M associated events and resulted in a reduced clonogenic survival upon taxol treatment. Taxol treatment resulted in elevated expression of p53 and of p21, which was more pronounced and persistent in cyclin D1 overexpressing cells. Overexpression of cyclin D1 altered sensitivity to taxol by modulating exit from mitosis, which is controlled by p21. These results indicate that overexpression of cyclin D1 sensitizes MCF7 cells to treatment with taxol.
Michalides
Rob
R
Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam. rmichal@nki.nl
Tiemessen
Machteld
M
Verschoor
Tom
T
Balkenende
Astrid
A
Coco-Martin
Jose
J
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D019938:Cyclin D1 / Q000096:biosynthesis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008938:Mitosis; D017239:Paclitaxel / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D019938:Cyclin D1; D017239:Paclitaxel