title: Helicobacter pylori infection in connective tissue disorders is associated with high levels of antibodies to mycobacterial hsp65 but not to human hsp60.
abstract: BACKGROUND
  To investigate whether the Helicobacter pylori status influences levels of antibodies against mycobacterial heat shock protein (hsp) 65 and human hsp60 in systemic autoimmune diseases and to study the concentration of anti-H. pylori antibodies in autoimmune patients and healthy controls.
  
  
  METHODS
  Antibodies against human heat-shock protein hsp60, mycobacterial heat-shock protein hsp65 were analyzed by ELISA. Anti-Helicobacter antibodies were determined by enzyme immunoassay.
  
  
  RESULTS
  There was a markedly higher prevalence of H. pylori infection in undifferentiated connective tissue disease (82%) (n = 33) and systemic sclerosis (78%) (n = 55) but not in systemic lupus erythematosus (n = 49), polymyositis/dermatomyositis (n = 14), rheumatoid arthritis (n = 21) or primary Raynaud's syndrome (n = 26) compared with controls (59%) (n = 349). In autoimmune diseases H. pylori infection was associated with elevated levels of antihsp65 (p =.008) but not of antihsp60. Anti-hsp65 levels were significantly higher in H. pylori-infected (n = 129) than in uninfected patients (n = 69) (p =.0007).
  
  
  CONCLUSIONS
  These findings indicate that in autoimmune diseases the infection with the H. pylori bacterium is associated with increased concentration of antimycobacterial hsp65.
authors.affiliation: 3rd Department of Internal Medicine, Faculty of Medicine, Semmelweis University and Research Group of Metabolism, Genetics and Immunology, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Kalabay
authors.forename: Béla
authors.initials: B
authors.lastname: Fekete
authors.forename: László
authors.initials: L
authors.lastname: Czirják
authors.forename: Laura
authors.initials: L
authors.lastname: Horváth
authors.forename: Mohamed R
authors.initials: MR
authors.lastname: Daha
authors.forename: Amarilla
authors.initials: A
authors.lastname: Veres
authors.forename: Gábor
authors.initials: G
authors.lastname: Fónyad
authors.forename: Anna
authors.initials: A
authors.lastname: Horváth
authors.forename: Agnes
authors.initials: A
authors.lastname: Viczián
authors.forename: Mahavir
authors.initials: M
authors.lastname: Singh
authors.forename: Izabella
authors.initials: I
authors.lastname: Hoffer
authors.forename: George
authors.initials: G
authors.lastname: Füst
authors.forename: László
authors.initials: L
authors.lastname: Romics
authors.forename: Z
authors.initials: Z
authors.lastname: Prohászka
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000276:immunology / Q000382:microbiology; D001426:Bacterial Proteins; D018834:Chaperonin 60 / Q000276:immunology*; D018833:Chaperonins / Q000276:immunology*; D003240:Connective Tissue Diseases / Q000276:immunology* / Q000382:microbiology; D005260:Female; D016481:Helicobacter Infections / Q000276:immunology / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D000907:Antibodies, Bacterial; D001323:Autoantibodies; D001426:Bacterial Proteins; D018834:Chaperonin 60; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins