The effects on pulp tissue of microleakage in resin composite restorations.
The purpose of this study was to evaluate histopathologically the effect on pulp tissue of microleakage in resin composite restorations. Seventy-two class V cavities were prepared on buccal surfaces of monkeys and divided into 3 groups, F, O, and S. Every cavity was etched with 37% phosphoric acid. In group F cavities, each cavity was restored with photo-curable composite without any dentin adhesives. In group O, the cavities were left unfilled. In group S, each cavity was treated with a dentin adhesive system and restored with a restorative composite. After 3, 30, or 90 days, animals were sacrificed and the subjected teeth were immediately removed, then fixed and decalcified. Following sectioning and staining with hematoxylin and eosin or Taylor's modified bacteria staining, each sample was examined with a light microscope. In most teeth with group S cavities, bacterial invasion was not found indicating excellent marginal sealing. The pulpal reaction was much less than that in other groups. In group F as well as in group O, bacteria were frequently observed in the cavity; however, bacteria penetrated into dentinal tubules more in group F than in group O at 30 and 90 days. A correlation between the presence of bacteria and pulpal inflammation was strongly indicated. It was suggested that a leaky restoration was more harmful to the pulp than an open prepared cavity without restoration.
Sasafuchi
Y
Y
Department of Operative Dentistry, Faculty of Dentistry, Tokyo Medical & Dental University, Japan. y.sasafuchi.odnt@dent.tmd.ac.jp
Otsuki
M
M
Inokoshi
S
S
Tagami
J
J
D000134:Acid Etching, Dental; D000704:Analysis of Variance; D000818:Animals; D001419:Bacteria / Q000145:classification; D004396:Coloring Agents; D003188:Composite Resins / Q000737:chemistry*; D003736:Dental Cavity Lining; D003737:Dental Cavity Preparation / Q000145:classification; D003763:Dental Leakage / Q000473:pathology*; D018772:Dental Marginal Adaptation; D003782:Dental Pulp / Q000382:microbiology / Q000473:pathology*; D003793:Dental Restoration, Permanent / Q000145:classification; D003804:Dentin / Q000382:microbiology / Q000473:pathology; D003809:Dentin, Secondary / Q000473:pathology; D017220:Dentin-Bonding Agents / Q000737:chemistry; D005456:Fluorescent Dyes; D008251:Macaca; D008689:Methacrylates / Q000737:chemistry; D009804:Odontoblasts / Q000473:pathology; D010756:Phosphoric Acids / Q000737:chemistry; D011671:Pulpitis / Q000382:microbiology / Q000473:pathology; D019279:Resin Cements / Q000737:chemistry; D013223:Statistics as Topic; D013499:Surface Properties; D013997:Time Factors
C112828:Clearfil AP-X; C112274:Clearfil Liner Bond 2; D004396:Coloring Agents; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D005456:Fluorescent Dyes; D008689:Methacrylates; D010756:Phosphoric Acids; C409388:Protect Liner F; D019279:Resin Cements