title: Biochemical Properties of Bound-Ca(2+) in Fibrinolytic Principle (FP) Separated from Agkistrodon acutus Venom.
abstract: It has been determined that each FP molecular contains one Ca(2+)-binding site. By the use of fluorescence probe Tb(3+), the distance between Tb(3+) and tryptophan (Trp) residue was obtained to be 0.375. Tb(3+) ion is coordinated with FP more strongly than Ca(2+) ion, and can bind to FP and replace the Ca(2+) ion in FP completely.
authors.affiliation: Department of Chemistry, University of Science and Technology of China, Hefei 230026, China. lab305@mail.ach.ustc.edu.cn
authors.forename: Shou-Ye
authors.initials: SY
authors.lastname: Wang
authors.forename: Zu-De
authors.initials: ZD
authors.lastname: Zhang
authors.forename: Zhen-Long
authors.initials: ZL
authors.lastname: Chen
authors.forename: Hua-Ming
authors.initials: HM
authors.lastname: Yu
authors.forename: Shuang-Ding
authors.initials: SD
authors.lastname: Wu
authors.forename: Qing-Liang
authors.initials: QL
authors.lastname: Liu