title: Biological effects of rutin on the survival of Escherichia coli AB1157 and on the electrophoretic mobility of plasmid PUC 9.1 DNA.
abstract: The use of natural products as medicines is growing in the world. The rutin, a compound isolated from Ruta graveolens, is a flavonoid, which has been suggested to have antioxidant properties and to reduce the triacylglycerol levels. In this study, plasmid desoxyribonucleic acid (DNA) was exposed to rutin (0.33, 10, 20, 30 microg/ml) in presence of stannous chloride (SnCl2), a reducing agent widely used to obtain radiopharmaceuticals labeled with technetium-99m. Samples of the plasmid DNA were analyzed through agarose gel electrophoresis. E. coli AB1157 culture was also incubated with rutin (3, 30, 50, 100 microg/ml) and the survival fractions were calculated. The results show that the rutin, in these concentrations, is not capable of: i/ damaging the DNA, ii/ protecting the DNA from the SnCl2 redox action, and iii/ inactivating the E. coli AB1157 culture. The analysis of our data indicates that rutin do not present toxic activity in the evaluated systems.
authors.affiliation: Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofisica e Biometria, RJ, Brazil. bernardo@uerj.br
authors.forename: Luciana C
authors.initials: LC
authors.lastname: Bernardo
authors.forename: Márcia B N
authors.initials: MB
authors.lastname: de Oliveira
authors.forename: Cláudia R
authors.initials: CR
authors.lastname: da Silva
authors.forename: Flávio J S
authors.initials: FJ
authors.lastname: Dantas
authors.forename: José C P
authors.initials: JC
authors.lastname: de Matros
authors.forename: Adriano
authors.initials: A
authors.lastname: Caldeira-de-Araújo
authors.forename: Roberto S
authors.initials: RS
authors.lastname: Moura
authors.forename: Mario
authors.initials: M
authors.lastname: Bernardo-Filho
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects; D024202:Electrophoretic Mobility Shift Assay; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D010957:Plasmids / Q000187:drug effects*; D032105:Ruta / Q000737:chemistry; D012431:Rutin / Q000494:pharmacology*; D017971:Tin Compounds
chemical_list: D000975:Antioxidants; D017971:Tin Compounds; C023599:stannous chloride; D012431:Rutin