title: Modelling of cell proliferation: nuclear versus membrane labelling.
abstract: Cell proliferation is a fundamental process involved in growth, development and oncogenesis. Monitoring and quantification of proliferation are essential to analyse the behaviour of cells drug-treated or not. Flow cytometry assessment of cell proliferation requires mathematical models to extract information of interest from fluorescence distributions. Various methods are available for cell cycle analysis, including estimation of cell phase durations and doubling time. In this context, we compare widely used flow cytometric methods based on nuclear labelling (using BrdUrd incorporation in combination with DNA content) to membrane labelling (using intercalating dyes PKH).
authors.affiliation: Laboratoire de dynamique cellulaire, Ecole pratique des hautes études, CNRS UMR 5525, institut Albert-Bonniot, 38706 La Tronche, France. Magali.Barbier@ujf-grenoble.fr
authors.forename: Magali
authors.initials: M
authors.lastname: Barbier
authors.forename: Jean-Fabien
authors.initials: JF
authors.lastname: Laurier
authors.forename: Daniel
authors.initials: D
authors.lastname: Seigneurin
authors.forename: Xavier
authors.initials: X
authors.lastname: Ronot
authors.forename: Jean
authors.initials: J
authors.lastname: Boutonnat
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D008433:Mathematics; D008954:Models, Biological
chemical_list: D004247:DNA; D001973:Bromodeoxyuridine