title: Unique case of Helicobacter sp. osteomyelitis in an immunocompetent child diagnosed by broad-range 16S PCR.
abstract: We report the first case of Helicobacter sp. osteomyelitis in an immunocompetent child. The infection was diagnosed by broad-range 16S PCR followed by sequencing of the resulting amplicon. All other microbiological investigations proved negative. This case highlights the importance of molecular methods in the diagnosis of unsuspected etiological agents and the potential role of Helicobacter sp. in human infection.
authors.affiliation: Department of Microbiology, Great Ormond Street Hospital for Children NHS Trust, London, United Kingdom. harrik2@gosh.nhs.uk
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Harris
authors.forename: Katy J
authors.initials: KJ
authors.lastname: Fidler
authors.forename: John C
authors.initials: JC
authors.lastname: Hartley
authors.forename: Julie
authors.initials: J
authors.lastname: Vogt
authors.forename: Nigel J
authors.initials: NJ
authors.lastname: Klein
authors.forename: Fergal
authors.initials: F
authors.lastname: Monsell
authors.forename: Vas M
authors.initials: VM
authors.lastname: Novelli
mesh_terms: D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D016998:Helicobacter / Q000235:genetics / Q000302:isolation & purification*; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D007121:Immunocompetence; D008969:Molecular Sequence Data; D010019:Osteomyelitis / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S