Expression of neurotrophin messenger RNAs during rat urinary bladder development.
The family of neurotrophins, encompassing nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5), is important in the regulation of neuronal development and function. We examined the expression of neurotrophin messenger RNAs (mRNAs) in the rat urinary bladder during pre- and postnatal development using competitive reverse transcription-polymerase chain reaction. The mRNA levels showed a biphasic pattern of expression; one peak was at prenatal ages (embryonic day (E)15-E18) and the other peak was at postnatal ages (postnatal day (P)14-P28). NT-4/5, BDNF and NGF mRNA levels were greatest at E15, E16 and E18, respectively. In contrast, NT-3 mRNA levels were significantly highest at P14. These data suggest that neurotrophins are involved in the mechanisms of bladder nerve growth for the prenatal period and reorganization of bladder reflex pathways during the second to the fourth postnatal week.
Kawakami
Takahiro
T
Department of Urology, Shiga University of Medical Science, Otsu 520-2192, Japan.
Wakabayashi
Yoshihiko
Y
Isono
Takahiro
T
Aimi
Yoshinari
Y
Okada
Yusaku
Y
D000367:Age Factors; D000818:Animals; D018507:Gene Expression Regulation, Developmental; D009475:Neurons, Afferent / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020813:Receptor, trkB / Q000235:genetics; D020812:Receptor, trkC / Q000235:genetics; D017475:Receptors, Nerve Growth Factor / Q000235:genetics*; D012018:Reflex / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001743:Urinary Bladder / Q000254:growth & development* / Q000294:innervation*; D014554:Urination / Q000502:physiology
D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor; C089191:neurotrophin 4(5) receptor; D020813:Receptor, trkB; D020812:Receptor, trkC