Restraint stress alters the expression of interleukin-1 and keratinocyte growth factor at the wound site: an in situ hybridization study.
Stress has a negative impact on wound healing. This murine study evaluated the effect of restraint stress (RST) on interleukin-1 (IL-1) and keratinocyte growth factor-1 (KGF-1) gene expression in cutaneous wounds by in situ hybridization. At day 1, RST mice had reduced frequency of IL-1beta mRNA-expressing fibroblasts compared to control mice. At day 3, RST mice had reduced frequencies of IL-1beta mRNA-expressing leukocytes, KGF-1 mRNA-expressing fibroblasts, and thin proliferating zones of epithelium. At day 5, RST mice had persistent leukocytic foci and reduced granulation tissue. Therefore, stress-induced alterations in cutaneous proinflammatory cytokine and growth factor expression are associated with significant histological changes in healing wounds.
Mercado
Ana M
AM
Department of Oral Biology, The Ohio State University, Columbus, OH 43210, USA.
Quan
Ning
N
Padgett
David A
DA
Sheridan
John F
JF
Marucha
Phillip T
PT
D000818:Animals; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D005260:Female; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors / Q000235:genetics*; D005347:Fibroblasts / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology; D017403:In Situ Hybridization; D007249:Inflammation / Q000235:genetics* / Q000276:immunology / Q000503:physiopathology; D007375:Interleukin-1 / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000276:immunology / Q000378:metabolism; D012149:Restraint, Physical; D012867:Skin / Q000276:immunology* / Q000293:injuries / Q000503:physiopathology; D013312:Stress, Physiological / Q000235:genetics* / Q000276:immunology / Q000503:physiopathology; D014945:Wound Healing / Q000235:genetics*
C496454:Fgf7 protein, mouse; D007375:Interleukin-1; D012333:RNA, Messenger; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors