Application of a Designed Synthetic Ligand for the Separation and Purification of Alkaline Phosphatase.
An artificial affinity ligand comprising a p-aminobenzyl phosphoric acid as inhibitor was designed and coupled to an agarose support by chlorotriazine ring to separate and purify alkaline phosphatase from calf intestine. The optimal density of the ligand is 6 &mgr;mol/g adsorbent. After elution with sodium chloride and inorganic phosphoric acid a 300-fold purified enzymatic preparation was obtained in one step with a yield of 90%.
Yang
Qing
Q
Institute of Biochemical Engineering, School of Chemical Engineering, Dalian University of Technology, Dalian 116012, China.
Yao
Wei
W
Peng
Ming-Li
ML
Gao
Kun-Yu
KY
Su
Zhi-Guo
ZG