Sphingomyelin content conditions insertion of daunorubicin within phosphatidylcholine monolayers.
Cell death induced by the chemotherapeutic drug daunorubicin (DNR) implicates an apoptotic pathway originating at the plasma membrane and characterized by sphingomyelin (SM) hydrolysis and ceramide generation. The mechanisms by which such a drug (hypothetically passively diffusing across a structural membrane) can trigger SM hydrolysis is unknown, but raises the question of the precise interaction between DNR and membrane lipid constituents. In this initial study, using alternative current polarography together with voltammetry, we report that after a first step of adsorption, insertion of DNR within a condensed phosphatidylcholine monolayer was significantly facilitated by SM content.
Lecompte
Marie-France
MF
Faculté de Médecine de Rangueil, INSERM U466, Toulouse, France.
Laurent
Guy
G
Jaffrézou
Jean-Pierre
JP
D000327:Adsorption; D003630:Daunorubicin / Q000737:chemistry* / Q000494:pharmacology; D004563:Electrochemistry; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008567:Membranes, Artificial; D010084:Oxidation-Reduction / Q000187:drug effects; D010713:Phosphatidylcholines / Q000737:chemistry*; D011048:Polarography; D013109:Sphingomyelins / Q000737:chemistry*; D013499:Surface Properties / Q000187:drug effects
D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D003630:Daunorubicin