Evaluation of antibody response to canine coronavirus infection in dogs by Western Blotting analysis.
We investigated by Western Blotting the antibody responses against the three major structural proteins of Canine coronavirus (CCoV) in dogs naturally infected. A pool of Elisa positive sera were also tested to clearly identify the binding profiles of CCoV proteins. The immune response to S protein was barely detectable in naturally infected dogs, whereas anti-M and anti-N antibodies were detected with a very strong reaction and for a long time post infection. The limited response to S protein may explain the poor protection of dogs and the possibility of persisting infection.
Elia
G
G
Department of Health and Animal Well-being, Faculty of Veterinary Medicine, Bari, Italy.
Decaro
N
N
Tinelli
A
A
Martella
V
V
Pratelli
A
A
Buonavoglia
C
C
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D015153:Blotting, Western / Q000662:veterinary; D018352:Coronavirus Infections / Q000276:immunology / Q000662:veterinary*; D017939:Coronavirus, Canine / Q000276:immunology / Q000378:metabolism*; D004283:Dog Diseases / Q000276:immunology / Q000821:virology*; D004285:Dogs; D005767:Gastrointestinal Diseases / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D015678:Viral Structural Proteins / Q000378:metabolism
D000914:Antibodies, Viral; D015678:Viral Structural Proteins