Survivin mRNA expression in patients with breast cancer.
We measured survivin mRNA expression in breast cancers using a fluorogenically detected reverse transcription-polymerase chain reaction, seeking relationships between expression and clinical and histological variables. The mean relative expression of survivin mRNA was 0.347+/-0.466 in breast cancers and 0.017+/-0.023 in noncancerous breast tissue resected with the tumors. A cut-off value for positivity was set at the mean + SD for expression in the noncancerous tissues; positivity for survivin mRNA expression was 90.2% (37 out of 41 cases) in breast cancers and 23% (3 out of 13) in adjacent noncancerous breast tissues. Expression in tumors varied independently of clinicopathological factors except for lymphatic invasion. As measured by our sensitive detection system, survivin mRNA is a useful diagnostic marker for breast cancer.
Nasu
Shunichi
S
Division of Laboratory Diagnosis, Sapporo Medical University School of Medicine, Japan.
Yagihashi
Atsuhito
A
Izawa
Akiko
A
Saito
Kaori
K
Asanuma
Koichi
K
Nakamura
Masashi
M
Kobayashi
Daisuke
D
Okazaki
Minoru
M
Watanabe
Naoki
N
D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D008875:Middle Aged; D009363:Neoplasm Proteins; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin
C107489:BIRC5 protein, human; D002868:Chromosomal Proteins, Non-Histone; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D000077022:Survivin