Shimotsu-to is the agent in Juzen-taiho-to responsible for the prevention of endometrial carcinogenesis in mice.
We have found that Juzen-taiho-to has a preventive effect on endometrial carcinogenesis in mice (Carcinogenesis 22 (2001) 587). In the present study, the constituents of Juzen-taiho-to responsible for this effect were explored using a short-term experiment. Thirty female ICR mice were divided into five groups: Group 1 was given a diet containing 0.2% of Juzen-taiho-to and 5ppm estradiol-17beta (E(2)); Group 2 was given a diet containing Shimotsu-to (0.07%) and E(2) (5ppm); Group 3 received Shikunshi-to (0.08%) and E(2) (5ppm) in the diet; Group 4 was given 5ppm E(2) in the diet; and Group 5 served as a control. Exposure of Juzen-taiho-to or Shimotsu-to decreased E(2)-stimulated expression of estrogen-related gene c-fos mRNA (P<0.05), and the cytokines interleukin-1alpha mRNA and tumor necrosis factor alpha mRNA P<0.01). A similar trend was not found upon treatment with Shikunshi-to. These findings suggest that Shimotsu-to is responsible for the inhibitory effects of Juzen-taiho-to on the estrogen-related endometrial carcinogenesis in mice.
Lian
Zenglin
Z
Department of Obstetrics & Gynecology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu-city 500-8705, Japan.
Niwa
Kenji
K
Gao
Jingchun
J
Tagami
Keiko
K
Hashimoto
Midori
M
Yokoyama
Yasuhiro
Y
Mori
Hideki
H
Tamaya
Teruhiko
T
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016889:Endometrial Neoplasms / Q000517:prevention & control*; D004958:Estradiol / Q000633:toxicity; D005260:Female; D016762:Genes, fos; D016758:Genes, jun; D007375:Interleukin-1 / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D016588:Anticarcinogenic Agents; D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C063587:juzentaihoto; C079710:shimotsu-to; D004958:Estradiol