Nogo-A expression in mouse central nervous system neurons.
The longest central nervous system (CNS) specific isoform of the major myelin-associated inhibitor of neurite growth, Nogo-A, has previously been shown to be expressed largely in oligodendrocytes. Using an antibody raised against a recombinant fusion protein comprising amino acids 223-399 of Nogo-A, we show in this report that Nogo-A is also expressed in the cell body of a distinct set of CNS neurons. The antibody detects a single protein band of 220 kDa in rat brain lysate and neuroblastoma cell lysates. Immunofluorescent analyses reveal that Nogo-A is found largely in the endoplasmic reticulum of neuroblastoma cell lines SH-SY5Y and NIE-115. In the mouse CNS, Nogo-A can be found in specific subsets of neuronal cell bodies in addition to oligodendrocytes, but not glial fibrillary associated protein positive astrocytes or MAC-1 positive microglia. Our results provide a conclusive demonstration of the expression of Nogo-A in CNS neurons, which suggests that Nogo-A may have distinct endogenous roles in neurons other than its known ability to inhibit neurite growth.
Liu
Haiping
H
NCA laboratory, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore, Singapore.
Ng
Cherry E L
CE
Tang
Bor Luen
BL
D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009185:Myelin Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D000070798:Nogo Proteins; D009836:Oligodendroglia / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603201:Rtn4 protein, mouse; C000603197:Rtn4 protein, rat