Modulatory effect of HHV-6 on MCP-1 production by human monocytes.
Chemokines represent a large family of proinflammatory proteins that orchestrate leukocyte trafficking to sites of viral infection. Human Herpes virus type 6 (HHV-6) is a typical immunosuppressive agent, as suggested by its tropism. In this study the production of monocyte chemoattractant protein-1 (MCP-1) and interleukin-10 (IL-10) by human peripheral blood monocytes was evaluated during HHV-6 infection. Our results demonstrate that HHV-6 infection triggers monocytes to release MCP-1 and IL-10. The addition of exogenous recombinant MCP-1 upregulates the release of extracellular virus, whereas does not influence the percentage of viral-antigen positive cells. Furthermore, the addition of monoclonal antibodies anti-IL-10 down-regulates MCP-1 release induced by HHV-6. These findings indicate that IL-10 and MCP-1 production was closely related and that the marked amounts of MCP-1 were supported not only by virus but also by virus-induced IL-10.
Arena
A
A
Department of Surgical Sciences, Policlinico Universitario, University of Messina, Italy.
Stassi
G
G
Speranza
A
A
Iannello
D
D
Mastroeni
P
P
D000956:Antigens, Viral / Q000276:immunology / Q000378:metabolism; D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000276:immunology* / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral / Q000276:immunology; D005786:Gene Expression Regulation / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology* / Q000378:metabolism; D015654:Herpesvirus 6, Human / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism / Q000821:virology*
D000956:Antigens, Viral; D018932:Chemokine CCL2; D016753:Interleukin-10