title: Thermodynamic parameters for an expanded nearest-neighbor model for the formation of RNA duplexes with single nucleotide bulges.
abstract: Thirty-four RNA duplexes containing single nucleotide bulges were optically melted, and the thermodynamic parameters deltaH degrees, deltaS degrees, deltaG degrees (37), and T(M) for each sequence were determined. Data from this study were combined with data from previous thermodynamic data [Longfellow, C. E., Kierzek, R., and Turner, D. H. (1990) Biochemistry 29, 278-85] to develop a model that will more accurately predict the free energy of an RNA duplex containing a single nucleotide bulge. Differences between purine and pyrimidine bulges as well as differences between Group I duplexes, those in which the bulge is not identical to either neighboring nucleotide, and Group II duplexes, those in which the bulge is identical to at least one neighboring nucleotide, were considered. The length of the duplex, non-nearest-neighbor effects, and bulge location were also examined. A model was developed which divides sequences into two groups: those with pyrimidine bulges and those with purine bulges. The proposed model for pyrimidine bulges predicts deltaG degrees (37,bulge) = 3.9 kcal/mol + 0.10deltaG degrees (37,nn) + beta, while the model for purine bulges predicts deltaG degrees (37,bulge) = 3.3 kcal/mol - 0.30deltaG degrees (37,nn) + beta, where beta has a value of 0.0 and -0.8 kcal/mol for Group I and Group II sequences, respectively, and deltaG degrees (37,nn) is the nearest-neighbor free energy of the base pairs surrounding the bulge. The conformation of bulge loops present in rRNA was examined. Three distinct families of structures were identified. The bulge loop was either extrahelical, intercalated, or in a "side-step" conformation.
authors.affiliation: Department of Chemistry, Allegheny College, Meadville, Pennsylvania 16335-3902, USA.
authors.forename: Brent M
authors.initials: BM
authors.lastname: Znosko
authors.forename: Sara B
authors.initials: SB
authors.lastname: Silvestri
authors.forename: Heather
authors.initials: H
authors.lastname: Volkman
authors.forename: Bob
authors.initials: B
authors.lastname: Boswell
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Serra
mesh_terms: D000241:Adenosine / Q000737:chemistry; D020029:Base Pairing; D019614:Haloarcula marismortui; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry*; D011685:Purine Nucleotides / Q000737:chemistry; D011742:Pyrimidine Nucleotides / Q000737:chemistry; D012335:RNA, Ribosomal / Q000737:chemistry*; D013816:Thermodynamics; D016963:Thermus thermophilus
chemical_list: D009692:Nucleic Acid Heteroduplexes; D009711:Nucleotides; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D012335:RNA, Ribosomal; D000241:Adenosine