Cloning and expression of an endo-1,6-beta-D-glucanase gene (neg1) from Neurospora crassa.
A gene (neg1) encoding an endo-1,6-beta-D-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-1,6-beta-D-glucanase protein of 463 amino acids and signal peptide of 17 amino acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-beta-D-glucanase activity. No genes similar in sequence were found in yeasts and fungi.
Oyama
Seiji
S
Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Yamagata
Youhei
Y
Abe
Keietsu
K
Nakajima
Tasuku
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000235:genetics
D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases