Peptides that elicit midgut stem cell differentiation isolated from chymotryptic digests of hemolymph from Lymantria dispar pupae.
Isolated stem cells of Heliothis virescens, cultured in vitro, were induced to differentiate by Midgut Differentiation Factors 3 and 4. These were peptides identified from a chymotrypsin digest of hemolymph taken from newly pupated Lymantria dispar. Partial purification was obtained by filtration through size exclusion filters. The most active preparation was subsequently subjected to a series of 3 Reverse Phase-HPLC procedures. Partial sequences of the peptides were identified via automated Edman degradation as the nanomers EEVVKNAIA-OH (MDF 3) and ITPTSSLAT-OH (MDF 4). These sequences were commercially synthesized. The synthetic compounds proved active in a dose-dependent manner. Stem cells responded to synthetic MDF 3 and MDF 4 as they did to previously identified peptides MDF 1 and 2, which have quite different amino acid sequences. All of the 4 MDFs administered singly induced statistically similar differentiation responses at 2 x 10(-8), 2 x 10(-9), and 2 x 10(-10) M. However, pairs of the 4 MDFs produced even more differentiation, the same response as one alone, no response, or were inhibitory, dependent on the MDF pair and its concentration. The data suggests complicated receptor interactions.
Loeb
Marcia J
MJ
Insect Biocontrol Laboratory, U.S. Department of Agriculture, Bldg 011A, Rm 214, BARC West, Beltsville, MD 20705, USA. loebm@ba.ars.usda.gov
Jaffe
Howard
H
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000737:chemistry / Q000378:metabolism; D004064:Digestive System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D006368:Helianthus; D006458:Hemolymph / Q000737:chemistry*; D019476:Insect Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology; D009036:Moths / Q000254:growth & development*; D020539:Sequence Analysis, Protein; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
D019476:Insect Proteins; D002918:Chymotrypsin