Molecular breeding of transgenic rice plants expressing a bacterial chlorocatechol dioxygenase gene.
The cbnA gene encoding the chlorocatechol dioxygenase gene from Ralstonia eutropha NH9 was introduced into rice plants. The cbnA gene was expressed in transgenic rice plants under the control of a modified cauliflower mosaic virus 35S promoter. Western blot analysis using anti-CbnA protein indicated that the cbnA gene was expressed in leaf tissue, roots, culms, and seeds. Transgenic rice calluses expressing the cbnA gene converted 3-chlorocatechol to 2-chloromucote efficiently. Growth and morphology of the transgenic rice plants expressing the cbnA gene were not distinguished from those of control rice plants harboring only a Ti binary vector. It is thus possible to breed transgenic plants that degrade chloroaromatic compounds in soil and surface water.
Shimizu
Masami
M
Faculty of Bioresources, Mie University, Tsu 514-8507, Japan.
Kimura
Tetsuya
T
Koyama
Takayoshi
T
Suzuki
Katsuhisa
K
Ogawa
Naoto
N
Miyashita
Kiyotaka
K
Sakka
Kazuo
K
Ohmiya
Kunio
K
D001673:Biodegradation, Environmental; D001947:Breeding / Q000379:methods*; D002396:Catechols / Q000378:metabolism; D020118:Cupriavidus necator / Q000201:enzymology / Q000235:genetics; D049308:Dioxygenases; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014170:Transformation, Genetic
D002396:Catechols; C031137:3-chlorocatechol; D010105:Oxygenases; D049308:Dioxygenases; C053101:catechol oxygenase II