title: Calcium uptake and calcium transporter expression by trophoblast cells from human term placenta.
abstract: Placental transfer of maternal calcium (Ca(2+)) is a crucial step for fetal development although the biochemical mechanisms responsible for this process are largely unknown. This process is carried out in vivo by the placental syncytiotrophoblast layer. The aim of this study was to define the membrane gates responsible for the syncytiotrophoblast Ca(2+) entry, the first step in transplacental transfer. We have investigated the basal Ca(2+) uptake by primary culture of human term placenta syncytiotrophoblast. Kinetic studies revealed an active extracellular Ca(2+) uptake by cultured human syncytiotrophoblast. We demonstrated by Northern blot the presence of transcript for calcium transporter type 1 (CaT1) in cultured human syncytiotrophoblast and CaT1 expression was further confirmed by reverse transcription polymerase chain reaction (RT-PCR). In addition, the expression of calcium transporter type 2 (CaT2) was revealed by RT-PCR in cultured human syncytiotrophoblast. It has been reported that the activity of this family of Ca(2+) channels is voltage-independent, and is not sensitive to L-type Ca(2+) channels agonist and antagonist. Interestingly, modulation of membrane potential by extracellular high potassium concentration and valinomycin had no effect on the basal Ca(2+) uptake of human syncytiotrophoblast. Moreover, the addition of L-type Ca(2+) channel modulators (Bay K 8644 and nitrendipine) to the incubation medium had also no effect on the basal Ca(2+) uptake, suggesting that the process is mainly voltage-independent and does not involved L-type Ca(2+) channels. On the other hand, we observed that two known blockers of CaT-mediated Ca(2+) transport, namely extracellular magnesium (Mg(2+)) and ruthenium red, dose-dependently inhibited Ca(2+) uptake by cultured human syncytiotrophoblast. Therefore, our results suggest that basal Ca(2+) uptake of human syncytiotrophoblast may be assured by CaT1 and CaT2.
authors.affiliation: Laboratoire de Physiologie materno-foetale, Département des Sciences Biologiques, Université du Québec à Montréal, C.P. 8888, Succursale "Centre-Ville", Montreal, Quebec, Canada H3C 3P8.
authors.forename: Robert
authors.initials: R
authors.lastname: Moreau
authors.forename: Georges
authors.initials: G
authors.lastname: Daoud
authors.forename: Renée
authors.initials: R
authors.lastname: Bernatchez
authors.forename: Lucie
authors.initials: L
authors.lastname: Simoneau
authors.forename: André
authors.initials: A
authors.lastname: Masse
authors.forename: Julie
authors.initials: J
authors.lastname: Lafond
mesh_terms: D026923:Amino Acid Transport Systems, Basic; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000096:biosynthesis / Q000378:metabolism*; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials; D010920:Placenta / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012430:Ruthenium Red / Q000494:pharmacology; D050916:TRPV Cation Channels; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism*
chemical_list: D026923:Amino Acid Transport Systems, Basic; D015220:Calcium Channels; C414177:SLC7A2 protein, human; D050916:TRPV Cation Channels; C120776:TRPV6 channel; D012430:Ruthenium Red; D008274:Magnesium; D002118:Calcium