Enhancement of circulating dendritic cell activity by immunomodulators (OK432 and KP-40).
The effects of biological response modifiers (BRMs) on the functions of two types of dendritic cells (DCs) were examined in relation to anti-tumor therapy. The two BRMs studied in our assay system were OK432 (a streptococcal preparation) and KP-40 (Propionibacterium avidum: a heat-inactivated bacterial vaccine). Recently, techniques for isolating human DCs from peripheral blood have been established, and DCs can be divided into two subsets: CD11c+ DCs (myeloid DC population) and CD11c- DCs (lymphoid DC population). Both OK432 and KP-40 were found to up-regulate the activity of myeloid-lineage DCs: the expression of major histocompatibility complex (MHC) class II molecules and adhesion/costimulatory molecules increased, and the production of IL-12 also increased. Therefore, DCs pulsed with OK432 and KP-40 could be applied to vaccination as a new adjuvant for specific immunotherapies.
Toyokawa
Hideyoshi
H
First Department of Surgery, Kansai Medical University, Moriguchi, Osaka, Japan.
Inaba
Muneo
M
Takai
Soichiro
S
Satoi
Sohei
S
Beuth
Josef
J
Ko
H L
HL
Matsui
Yoichi
Y
Kwon
A-Hon
AH
Kamiyama
Yasuo
Y
Ikehara
Susumu
S
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D001428:Bacterial Vaccines; D002478:Cells, Cultured; D003712:Dendrites / Q000187:drug effects / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D010844:Picibanil / Q000494:pharmacology*; D015164:Vaccines, Inactivated / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001428:Bacterial Vaccines; C465012:KP 40 vaccine; D015164:Vaccines, Inactivated; D010844:Picibanil