Development of a bipartite method for Fusarium identification based on cellobiohydrolase-C: CAPS and Western blot analysis.
The identification of 12 Fusarium strains isolated from diseased hops (Humulus lupulus, L.) was achieved by a strategy based on cellobiohydrolase-C: cleaved amplified polymorphic sequence analysis targeting the gene and the use of an antibody directed against a peptide of the Fusarium graminearum enzyme. This strategy is shown to be rapid and reliable for all the Fusarium of our collection: F. avenaceum, F. graminearum, F. sambucinum, F. sporotrichioides, F. tricinctum and F. venenatum.
Hatsch
Didier
D
U.M.R. 7100, Université Louis Pasteur-CNRS, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brant, 67400 Illkirch-Graffenstaden, France.
Phalip
Vincent
V
Jeltsch
Jean-Marc
JM
D015153:Blotting, Western; D002480:Cellulase / Q000032:analysis* / Q000235:genetics; D043366:Cellulose 1,4-beta-Cellobiosidase; D004271:DNA, Fungal / Q000032:analysis*; D005670:Fusarium / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D005784:Gene Amplification; D016133:Polymerase Chain Reaction
D004271:DNA, Fungal; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase