[Tumor producing amylase].
The phenotype of tumor-producing amylase is usually salivary-type amylase, in contrast, we experienced sialyl salivary-type amylase detected in the sera of the patients with malignancies. Sialyl salivary-type amylase, with an abnormal anodic migration, was detected by isoamylase electrophoretic analysis in the sera of patients with multiple myeloma and with ovarian cancer. The abnormal isoamylase bands migrated toward the anode faster than the salivary isoamylase band(S2) and were stained more strongly than S2 band. The isoamylases could be separated from residual normal isoamylases in the sera of patients by using gel permeation chromatography. The isoamylases were showed sensitive by neuraminidase treatment and were reacted with anti-human salivary monoclonal antibody.
Moriyama
Takanori
T
Department of Laboratory Technology, College of Medical Technology, Hokkaido University, Sapporo 060-0812.
Tozawa
Tatsuo
T
Matsuno
Kazuhiko
K
D000368:Aged; D000681:Amylases / Q000096:biosynthesis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000201:enzymology*; D010051:Ovarian Neoplasms / Q000201:enzymology*
D000681:Amylases