Organo-silica nano-particles used in ultrahigh-pressure liquid chromatography.
A simple one-step process was used to synthesize uniform, spherical organosilica nano-particles containing octadecyl moieties. These nano-particles, having a diameter of 670 nm, were slurry packed into fused-silica capillary tubes of 50 microm internal diameter and tested for use in ultrahigh-pressure liquid chromatography (UHPLC) at inlet pressures of about 50,000 psi (approximately 3,500 bar), providing for the use of HPLC with nano-particle packed columns. The retention characteristics of a column packed with the organo-nano-particles were shown to be stable under acidic (pH < 1) and basic (pH > 11) conditions. Fast analysis times and relatively high separation efficiencies (approximately 500,000 plates m(-1)) were obtained under the conditions used.
Cintrón
José M
JM
Department of Chemistry, University at Buffalo, The State University of New York, 14260-3000, USA.
Colón
Luis A
LA
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008854:Microscopy, Electron; D008863:Microspheres; D036103:Nanotechnology; D017646:Organosilicon Compounds
D017646:Organosilicon Compounds