Effect of acetazolamide on hypericin photocytotoxicity.
The photodynamic action of hypericin (HYP) in vitro was evaluated using human leukemic HL-60 and lung carcinoma A549 cell lines. After illumination HYP (1 x 10 (-5) M) reduced the proliferation and/or survival of HL-60 and A549 cells vs. controls to almost to 0 % and 29 %, respectively. A lower concentration of HYP (1 x 10 (-6) M) decreased the proliferation and/or survival only in HL-60 cells. Non-cytotoxic concentrations of the carbonic anhydrase inhibitor acetazolamide (ACTZ) (1 x 10 (-3)-1 x 10 (-6) M) significantly potentiated these effects of HYP (1 x 10 (-6)M) in HL-60, but not in the A549 cell line. The highest concentration of ACTZ (1 x 10 (-3) M) also induced an increase of the subdiploid G 0 /G 1 population in HYP (1 x 10 (-6) M) treated HL-60 cells from 14 % to 24 %. The results indicate that the photogenerated pH drop may participate in the potentiation of the photodynamic action of HYP observed in leukemia cells.
Solár
Peter
P
Cavarga
Ivan
I
Hofmanová
Jirina
J
Cekanová-Figurová
Mária
M
Miskovský
Pavol
P
Brezáni
Peter
P
Hrcková
Gabriela
G
Kozubík
Alois
A
Fedorocko
Peter
P
D000086:Acetazolamide / Q000494:pharmacology*; D000873:Anthracenes; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017484:Dermatitis, Phototoxic; D004347:Drug Interactions; D018922:HL-60 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000873:Anthracenes; D000970:Antineoplastic Agents; D002257:Carbonic Anhydrase Inhibitors; D010569:Perylene; C004965:hypericin; D000086:Acetazolamide