Direct wiring of cytochrome c's heme unit to an electrode: electrochemical studies.
A novel strategy for the immobilization of cytochrome c on the surface of chemically modified electrodes is demonstrated and used to investigate the protein's electron-transfer kinetics. Mixed monolayer films of alkanethiols and omega-terminated alkanethiols (terminated with pyridine, imidazole, or nitrile groups that are able to ligate with the heme) are used to adsorb cytochrome c to the surface of gold electrodes. The use of mixed films, as opposed to pure films, allows the concentration of adsorbed cytochrome to remain dilute and ensures a higher degree of homogeneity in their environment. The adsorbed protein is studied using electrochemical methods and scanning tunneling microscopy.
Wei
Jianjun
J
Contribution from the Chemistry Department, University of Pittsburgh, Pennsylvania 15260, USA.
Liu
Haiying
H
Dick
Allison R
AR
Yamamoto
Hiromichi
H
He
Yufan
Y
Waldeck
David H
DH
D003574:Cytochrome c Group / Q000737:chemistry*; D004563:Electrochemistry; D006418:Heme / Q000737:chemistry*
D003574:Cytochrome c Group; D006418:Heme