Solution structures of antimalarial drug-heme complexes.
Paramagnetic metal centers [such as Fe(III) found within ferriprotoporphyrin IX heme (FPIX)] exert through space effects on the relaxation rate of nearby proton spins that depend critically on the metal-proton distance. We have measured these effects for all protons of several antimalarial drugs that bind to FPIX by systematically varying the drug:heme molar ratio in high field NMR experiments. These measurements allow us to determine precise FPIX Fe-drug H distances for the solution structures of noncovalent complexes formed between FPIX mu-oxo dimers and the antimalarial drugs chloroquine (CQ), quinine (QN), and quinidine (QD). Using these distances, we then performed distance restraint calculations to determine the lowest-energy solution structures of these complexes. Structures were solved for neutral, monoprotic (+1), and diprotic (+2) forms of the drugs. Analysis of these structures allows us to visualize for the first time the stereospecific differences between QN and QD binding to FPIX and the differences in populations of QN and QD solution structures upon changes in digestive vacuolar pH for drug resistant malarial parasites [Dzekunov, S. M., et al. (2000) Mol. Biochem. Parasitol. 110, 107-124]. The data indicate a previously unrecognized key role for the CQ aliphatic chain in stabilizing FPIX-CQ complexes, and suggest how lengthening or shortening the chain might perturb stability. We also define FPIX:drug stoichiometries of 2:1 for the complexes formed at physiological FPIX concentrations, in contrast to the 4:1 and 5:1 stoichiometries previously determined at higher FPIX concentrations [Dorn, A., et al. (1998) Biochem. Pharmacol. 55, 727-736]. These atomic resolution antimalarial drug-heme structures should help elucidate how these drugs inhibit formation of hemozoin during metabolism of heme within the malarial parasite Plasmodium falciparum and assist ongoing development of strategies for circumventing antimalarial drug resistance.
Leed
Alison
A
Department of Chemistry, Lombardi Cancer Center, Georgetown University, Washington, D.C. 20057, USA.
DuBay
Kateri
K
Ursos
Lyann M B
LM
Sears
Devin
D
De Dios
Angel C
AC
Roepe
Paul D
PD
D000634:Aminoquinolines / Q000737:chemistry*; D000962:Antimalarials / Q000737:chemistry*; D001665:Binding Sites; D002738:Chloroquine / Q000737:chemistry; D019281:Dimerization; D006427:Hemin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011522:Protons; D011802:Quinidine / Q000737:chemistry; D011803:Quinine / Q000737:chemistry; D012996:Solutions; D013816:Thermodynamics
D000634:Aminoquinolines; D000962:Antimalarials; D046911:Macromolecular Substances; D011522:Protons; D012996:Solutions; D006427:Hemin; D002738:Chloroquine; D011803:Quinine; D011802:Quinidine