title: Moderate physical exercise induces the oxidation of human blood protein thiols.
abstract: Exercise is known to induce the oxidation of blood low-molecular-weight (LMW) thiols such as reduced glutathione (GSH). We previously reported that full-marathon running induced a decrease in human plasma levels of protein-bound sulfhydryl groups (p-SHs). Moderate exercise, a 30-min running at the intensity of the individual ventilatory threshold, performed by untrained healthy females caused a significant decrease in erythrocyte levels of p-SHs (mostly hemoglobin cysteine residues) and LMW thiols, but their levels returned to each baseline by 2 h. No significant change in plasma LMW thiols was observed. However, plasma levels of p-SHs significantly decreased after running and remained unchanged after 24 h. These results suggest that moderate exercise causes the oxidation of blood thiols, especially protein-bound thiols.
authors.affiliation: National Institute of Health and Nutrition, Tokyo, Japan. tinayama@mail.cc.tohoku.ac.jp
authors.forename: Takayo
authors.initials: T
authors.lastname: Inayama
authors.forename: Jun
authors.initials: J
authors.lastname: Oka
authors.forename: Misato
authors.initials: M
authors.lastname: Kashiba
authors.forename: Makoto
authors.initials: M
authors.lastname: Saito
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Higuchi
authors.forename: Keizo
authors.initials: K
authors.lastname: Umegaki
authors.forename: Yorihiro
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Matsuda
mesh_terms: D000328:Adult; D015308:Anaerobic Threshold / Q000502:physiology; D001798:Blood Proteins / Q000378:metabolism*; D003402:Creatine Kinase / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D008970:Molecular Weight; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; D013438:Sulfhydryl Compounds; D003402:Creatine Kinase