Separation and Electrophoretic Behaviour of 8-aminonaphthalene-1,3,6-trisulfonic Acid-derivatized Oligosaccharides by Capillary Electrophoresis.
The mixture of the hydrolyzate of dextran was derivatized with 8-aminonaphthalene-1,3,6-trisulfonic acid(ANTS) by reductive amination, then the derivatives were separated by capillary zone electrophoresis in 50 mmol/L phosphate, pH 2.5 electrophoretic buffer and in the buffer of 100mmol/L disodium tetraborate, pH 9.3, respectively. Two-dimensional mapping of the series of linear dextranoligosaccharide derivatives was obtained by plotting their relative electrophoretic mobilities in acidic buffer vs those in alkaline buffer. The relative electrophoretic mobilities of the derivatives were linearly correlated to the negative two-thirds power of the molecular weights. The effects of triethylamine (TEA) added into the electrophoretic buffer on the electrophoretic behavior of the oligosaccharide derivatives were discussed. Those effects may arise from the interaction between the oligosaccharide derivatives and TEA. Similar results were found when we applied the methods above to mannan-, xylan- and chitin-oligosaccharide derivatives, respectively.
Che
Fa-Yun
FY
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
Liu
Zhen-Yu
ZY
Wang
Kei-Yi
KY
Xia
Qi-Chang
QC