Does radiotherapy affect the outcome of the comet assay?
This study was designed to assess possible effects of fractionated radiotherapy (5 or 10 fractions at 2 Gy per fraction) on the DNA repair capacity of lymphocytes, as measured by the comet assay. 50 patients with various tumour types were chosen. They had received no chemotherapy during the 6 months prior to radiotherapy and did not receive cortisone. 10 ml of heparinized blood was collected before radiotherapy, after 5 fractions and after 10 fractions. Lymphocytes were isolated and analysed using the comet assay. On average, no effect on DNA repair capacity was observed that could be attributed to radiotherapy. On an individual basis, there were a few patients who showed a comparatively pronounced variability in their response to radiotherapy (three patients with a relative coefficient of variability of more than 30%). There was some indication of a weak correlation between poor repair capacity and severe side effects in normal tissue. We also found that alcohol in particular, and smoking to some extent, may impair repair capacity during radiotherapy. Age, gender, field size, medication and tumour entity showed no effect on repair capacity.
Müller
W-U
WU
Institut für Medizinische Strahlenbiologie, Universitätsklinikum Essen, D45122 Essen, Germany.
Bauch
T
T
Streffer
C
C
von Mallek
D
D
D000428:Alcohol Drinking / Q000009:adverse effects; D020552:Comet Assay; D004260:DNA Repair / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000528:radiation effects*; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D009369:Neoplasms / Q000473:pathology / Q000532:radiotherapy; D011836:Radiation Tolerance; D011878:Radiotherapy / Q000009:adverse effects* / Q000379:methods; D012907:Smoking / Q000009:adverse effects
D004273:DNA, Neoplasm