Ultra-rapid freezing and storage of rat embryos in an electric refrigerator at -130 degree C without liquid cryo-agents, with ultra-short exposure in the freezing medium and direct rehydration after thawing.
An ultra-rapid freezing technique for embryos has been developed. This procedure involves: ultra-short exposure to cryoprotectants; freezing of embryos in a metallic powder pre-cooled in an electric refrigerator at -130 degree C; storage of embryos ina refrigerator at -130 degree C; direct rehydration after thawing.
Isachenko
V V
VV
Kharkov Biotechnical Center, Kharkov, P.O.Kulinichi, 312120 Ukraine.
Alabart
J L
JL
Isachenko
E F
EF
Bezugly
N D
ND
Michelmann
H W
HW