Capillary electrophoresis with wavelength-resolved laser-induced fluorescence detection.
Capillary electrophoresis (CE) enables rapid separations with high separation efficiency and compatibility with small sample volumes. Laser-induced fluorescence detection can result in extremely low limits of detection in CE. Single-channel fluorescence detection, however, furnishes little qualitative information about a species being detected, except for its CE migration time. Use of multidimensional information often enables unambiguous identification of analytes. Combination of CE with information-rich wavelength-resolved fluorescence detection is analogous with ultraviolet-visible diode-array detection and furnishes both qualitative and quantitative chemical information about target species. This review discusses recent advances in wavelength-resolved laser-induced fluorescence detection coupled with CE, with an emphasis on instrument design.
Zhang
Xin
X
Department of Chemistry, University of Illinois, Urbana IL 61801, USA.
Stuart
Jeffrey N
JN
Sweedler
Jonathan V
JV
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods; D007834:Lasers; D010455:Peptides / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D010455:Peptides