Sodium metasilicate hypersensitivity in BALB/c mice.
BACKGROUND
Sodium metasilicate (SMS) is a key ingredient for a number of industrial and consumer products. Although little is known about potential for this chemical to cause allergic reactions, a similar silicate compound, sodium silicate, was reported to elicit IgE-mediated contact urticaria.


OBJECTIVE
The aim of this study was to evaluate the potential for sodium metasilicate to elicit an allergic response in female BALB/c mice after dermal exposure.


METHODS
The primary irritancy assay (IA), local lymph node assay (LLNA), and a mouse ear swelling test (MEST) were used to evaluate the hypersensitivity response elicited by SMS exposure. An evaluation of lymph node subpopulations, cytokine mRNA expression, and serum IgE levels was also conducted.


RESULTS
SMS caused significant dermal irritation at concentrations >or=6% and an allergic response after mice were sensitized with 4% SMS then challenged with 6% SMS in the MEST. Lymph node cell proliferation was not observed in the LLNA after treatment with SMS (2% to 6% SMS). Increases in lymph node cellularity, the percentage of B cells, and the expression of certain cytokine mRNAs were observed in mice treated with SMS. Changes in the concentration of serum IgE after SMS treatment, however, were not observed.


CONCLUSIONS
SMS appears to elicit a chemical hypersensitivity response in mice, as indicated by the MEST, but not by the LLNA. Increases in auricular lymph node cellularity, the percentage of B cells, and certain cytokine mRNAs support classifying SMS as a weak chemical allergen.
Karrow
N A
NA
Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA 23298, USA.
Guo
T L
TL
Leffel
E K
EK
Zhang
L X
LX
McCay
J A
JA
Germolec
D R
DR
White
K L
KL
D000485:Allergens; D000818:Animals; D002452:Cell Count; D002455:Cell Division; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003877:Dermatitis, Contact / Q000175:diagnosis / Q000209:etiology; D017453:Dermatitis, Irritant / Q000209:etiology; D004139:Dinitrofluorobenzene / Q000633:toxicity; D004431:Ear, External; D004487:Edema / Q000139:chemically induced; D005260:Female; D006967:Hypersensitivity / Q000175:diagnosis* / Q000209:etiology; D007073:Immunoglobulin E / Q000097:blood; D023441:Local Lymph Node Assay; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis; D017640:Silicates / Q000633:toxicity*
D000485:Allergens; D016207:Cytokines; D012333:RNA, Messenger; D017640:Silicates; C025349:sodium metasilicate; D007073:Immunoglobulin E; D004139:Dinitrofluorobenzene