Analysis of HIV-1- and CMV-specific memory CD4 T-cell responses during primary and chronic infection.
CD4 T-cell-specific memory antiviral responses to human immunodeficiency virus type 1 (HIV-1) and cytomegalovirus (CMV) were investigated in 16 patients with documented primary HIV-1 infection (4 of the 16 subjects also had primary CMV infection) and compared with those observed in patients with chronic HIV-1 and CMV coinfection. Virus-specific memory CD4 T cells were characterized on the basis of the expression of the chemokine receptor CCR7. HIV-1- and CMV-specific interferon-gamma-secreting CD4 T cells were detected in patients with primary and chronic HIV-1 and CMV coinfection and were mostly contained in the cell population lacking expression of CCR7. The magnitude of the primary CMV-specific CD4 T-cell response was significantly greater than that of chronic CMV infection, whereas there were no differences between primary and chronic HIV-1-specific CD4 T-cell responses. A substantial proportion of CD4(+)CCR7(-) T cells were infected with HIV-1. These results advance the characterization of antiviral memory CD4 T-cell response and the delineation of the potential mechanisms that likely prevent the generation of a robust CD4 T-cell immune response during primary infection.
Harari
Alexandre
A
Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland.
Rizzardi
G Paolo
GP
Ellefsen
Kim
K
Ciuffreda
Donatella
D
Champagne
Patrick
P
Bart
Pierre-Alexandre
PA
Kaufmann
Daniel
D
Telenti
Amalio
A
Sahli
Roland
R
Tambussi
Giuseppe
G
Kaiser
Laurent
L
Lazzarin
Adriano
A
Perrin
Luc
L
Pantaleo
Giuseppe
G
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000276:immunology*; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002908:Chronic Disease; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000150:complications / Q000276:immunology*; D005260:Female; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D008297:Male; D010641:Phenotype; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000032:analysis; D019562:Viral Load; D014766:Viremia
D000914:Antibodies, Viral; C515370:CCR7 protein, human; D054400:Receptors, CCR7; D019707:Receptors, Chemokine