Immunocytochemical demonstration of glucagon-like peptides in Mytilus edulis cerebral ganglia and an in vitro effect of vertebrate glucagon on glycogen metabolism.
Immunological detection of glucagon-like peptides was performed in the cerebral ganglia of the mussel Mytilus edulis using an anti-vertebrate glucagon antibody. Two clusters of positive neurosecretory cells were observed, as well as stained nervous fibers. The effect of vertebrate glucagon on glucose incorporation into glycogen of reserve cells was tested using an in vitro microplate bioassay. Optimal incubation conditions were previously defined and an inhibitory effect of porcine glucagon was obtained for concentrations ranging from 10(-6) to 10(-9)M. It is postulated that the glucagon-like peptide may be implicated in the regulation of glucose metabolism in bivalves.
Kellner
K
K
Laboratoire de Biologie et Biotechnologies Marines, Université de Caen, Esplanade de la paix, 14032 Caen Cedex, France. kellner@ibba.unicaen.fr
Heude-Berthelin
C
C
Mathieu
M
M
D000818:Animals; D049872:Bivalvia / Q000378:metabolism*; D017952:Ganglia, Invertebrate / Q000378:metabolism*; D005934:Glucagon / Q000494:pharmacology / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000378:metabolism; D009490:Neurosecretory Systems / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D013552:Swine
D010455:Peptides; D006003:Glycogen; D005934:Glucagon; D005947:Glucose