title: Completely autotrophic nitrogen removal over nitrite in one single reactor.
abstract: The microbiology and the feasibility of a new, single-stage, reactor for completely autotrophic ammonia removal were investigated. The reactor was started anoxically after inoculation with biomass from a reactor performing anaerobic ammonia oxidation (Anammox). Subsequently, oxygen was supplied to the reactor and a nitrifying population developed. Oxygen was kept as the limiting factor. The development of a nitrifying population was monitored by Fluorescence In Situ Hybridization and off-line activity measurements. These methods also showed that during steady state, anaerobic ammonium-oxidizing bacteria remained present and active. In the reactor, no aerobic nitrite-oxidizers were detected. The denitrifying potential of the biomass was below the detection limit. Ammonia was mainly converted to N2 (85%) and the remainder (15%) was recovered as NO3-. N2O production was negligible (less than 0.1%). Addition of an external carbon source was not needed to realize the autotrophic denitrification to N2.
authors.forename: A Olav
authors.initials: AO
authors.lastname: Sliekers
authors.forename: N
authors.initials: N
authors.lastname: Derwort
authors.forename: J L Campos
authors.initials: JL
authors.lastname: Gomez
authors.forename: M
authors.initials: M
authors.lastname: Strous
authors.forename: J G
authors.initials: JG
authors.lastname: Kuenen
authors.forename: M S M
authors.initials: MS
authors.lastname: Jetten
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001421:Bacteria, Anaerobic / Q000502:physiology*; D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D009573:Nitrites / Q000737:chemistry*; D009584:Nitrogen / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D009573:Nitrites; D002244:Carbon; D000641:Ammonia; D009584:Nitrogen