Effects of apigenin on the serum- and platelet derived growth factor-BB-induced proliferation of rat aortic vascular smooth muscle cells.
The inhibitory effects of apigenin on the growth factor-induced proliferative responses, and expression of mitogen-activated protein (MAP) kinase and its downstream c-fos in rat aortic vascular smooth muscle cells (VSMCs) were investigated. Apigenin significantly inhibited both 5 % fetal bovine serum (FBS)- and 50 ng/mL platelet derived growth factor-BB (PDGF-BB)-induced proliferation on primary cultured rat VSMCs in a concentration-dependent manner. In addition, apigenin resulted in a significant inhibition of the FBS-induced phosphorylation of extracellular signal-regulated kinase 1/2 (ERK 1/2) and expression of c-fos mRNA. These results suggest that apigenin inhibits FBS- and PDGF-BB-induced VSMC proliferation, and its activity may be mediated, at least in part, by down regulation of ERK 1/2 and its downstream c-fos mRNA.
Kim
Tack-Joong
TJ
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Zhang
Yong-He
YH
Kim
Youngsoo
Y
Lee
Chong-Kil
CK
Lee
Myung-Koo
MK
Hong
Jin-Tae
JT
Yun
Yeo-Pyo
YP
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001011:Aorta; D047310:Apigenin; D000077214:Becaplermin; D002455:Cell Division / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats
D000970:Antineoplastic Agents; D005419:Flavonoids; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D047310:Apigenin