title: Identification of genes regulating colorectal carcinogenesis by using the algorithm for diagnosing malignant state method.
abstract: We studied the expression profiles of various stages of colorectal tumors (adenoma (AD), seven samples; carcinoma (CA), 16 samples) by using cDNA microarrays and developed ADMS (algorithm for diagnosing malignant state) method, selecting 335 clones characteristic of CA state. We, then, applied ADMS to 12 additional samples (five from primary lesions with metastasis and seven metastases); all 16 CAs and 12 metastatic tumors were diagnosed correctly as cancerous states. Although three of the seven ADs were diagnosed as "cancerous," the large size of two of these tumors suggested their potential malignancy. Our strategy for selecting clones characteristic of the malignant state is widely applicable to diagnosis and for predicting the stage of progression during multistep carcinogenesis. Of the 335 clones we selected, 135 were known genes. Included in the 135 genes were tumor suppressor and growth factor-related genes and were consistent with the literature. ADMS is a reliable means for identifying genes useful for the diagnosis of cancer.
authors.affiliation: Second Department of Surgery, School of Medicine, Yokohama City University, Yokohama, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Shinji
authors.initials: S
authors.lastname: Takahashi
authors.forename: Youhei
authors.initials: Y
authors.lastname: Hamaguchi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Morita
authors.forename: Itaru
authors.initials: I
authors.lastname: Nishizuka
authors.forename: Shigeki
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Itaru
authors.initials: I
authors.lastname: Endo
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ike
authors.forename: Shinji
authors.initials: S
authors.lastname: Togo
authors.forename: Shigeo
authors.initials: S
authors.lastname: Oki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimada
authors.forename: Koji
authors.initials: K
authors.lastname: Kadota
authors.forename: Shugo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Goto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nitanda
authors.forename: Susumu
authors.initials: S
authors.lastname: Satomi
authors.forename: Takehito
authors.initials: T
authors.lastname: Sakai
authors.forename: Ichiei
authors.initials: I
authors.lastname: Narita
authors.forename: Fumitake
authors.initials: F
authors.lastname: Gejyo
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tomaru
authors.forename: Kentaro
authors.initials: K
authors.lastname: Shimizu
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okazaki
mesh_terms: D000236:Adenoma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D002277:Carcinoma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D018450:Disease Progression; D020869:Gene Expression Profiling; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis