A subpopulation of large granular von Willebrand Ag negative and CD105 positive endothelial cells, isolated from abdominal aortic aneurysms, overexpress ICAM-1 and Fas antigen.
The aim of this work was to determine whether there is a pre-established basal condition of the endothelial cells isolated from aortic abdominal aneurysm that might augment immune effector mechanisms and thus provide us an insight into the possible causes of aneurysm rupture. Endothelial cells isolated from saccular aortic aneurysm fragments were analyzed by cytofluorometry for the expression of different immune response-related molecules. Our results showed that there is a subpopulation of granule-rich, CD105 positive and von Willebrand antigen negative endothelial cells that have an enhanced basal expression of ICAM-1, and Fas antigen, but, interestingly, no apoptotic bodies were detected. Control endothelial cells derived from healthy areas of the same abdominal aortas did not show such enhanced expression. We conclude that in the endothelium that lines abdominal aorta aneurysms there is, at least, one endothelial cell subpopulation with an apparent inhibition of programmed cell death and in a proinflammatory activation status.
Páez
Araceli
A
Depto. Biología Celular, Instituto Nacional de Cardiología Ignacio Chávez, INCICH, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, 14080 México, D. F.
Archundia
Abel
A
Méndez Cruz
René
R
Rodríguez
Emma
E
López Marure
Rebeca
R
Masso
Felipe
F
Aceves
José Luis
JL
Flores
Leopoldo
L
Montaño
Luis F
LF
D000941:Antigens / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D001012:Aorta, Abdominal / Q000166:cytology; D017544:Aortic Aneurysm, Abdominal / Q000276:immunology* / Q000473:pathology; D017209:Apoptosis; D002478:Cells, Cultured; D003470:Culture Media; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D010641:Phenotype; D019014:fas Receptor / Q000276:immunology; D014841:von Willebrand Factor / Q000276:immunology
D000941:Antigens; D015703:Antigens, CD; D003470:Culture Media; D019014:fas Receptor; D014841:von Willebrand Factor; D018799:Intercellular Adhesion Molecule-1