title: Interpretation of RET immunostaining in follicular lesions of the thyroid.
abstract: We applied monoclonal antibodies against RET and cytokeratin 19 (CK19) to the following tumor sections: classic papillary carcinoma (PC), 16; Hürthle-type PC (HPC), 1; sclerosing PC with nodular fasciitis-like stroma (SPC), 1; PC, follicular variant (FVPC), 12; follicular adenoma (FA), 9; Hürthle cell adenoma (HA), 4; Hürthle cell carcinoma (HC), 3; and follicular carcinoma (FC), 7. CK19+ tumors included 16 PCs, 1HPC, 1SPC, 11 FVPCs, 7 FAs, 4 FCs, and 1HC. RET+ tumors included 4 HAs, 3 HCs, 1HPC, 12 PCs, 7 FVPCs, and 2 FAs. Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed a RET transcript in 6 Hürthle cell lesions. RET immunoreactivity is less sensitive and specific for PC than CK19. CK19 is useful for identifying PC, although only lesions with diffuse, intense staining should be considered positive. The detection of RET protein by immunohistochemical analysis was corroborated by the presence of the RET transcript by RT-PCR. Further study is warranted to determine whether this represents activation by gene fusion or some other mechanism in this subset of thyroid neoplasms.
authors.affiliation: Robert E Fechner Laboratory of Surgical Pathology, University of Virginia Health Sciences Center, Charlottesville, USA.
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Cerilli
authors.forename: Stacey E
authors.initials: SE
authors.lastname: Mills
authors.forename: Craig A
authors.initials: CA
authors.lastname: Rumpel
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Dudley
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Moskaluk
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000737:chemistry / Q000473:pathology*; D000236:Adenoma; D018249:Adenoma, Oxyphilic / Q000737:chemistry / Q000473:pathology; D000911:Antibodies, Monoclonal; D019677:Artificial Gene Fusion; D002291:Carcinoma, Papillary / Q000737:chemistry / Q000473:pathology*; D029721:Drosophila Proteins; D006801:Humans; D007633:Keratins / Q000032:analysis; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D051096:Proto-Oncogene Proteins c-ret; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013964:Thyroid Neoplasms / Q000737:chemistry / Q000473:pathology*; D014158:Transcription, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D029721:Drosophila Proteins; D011518:Proto-Oncogene Proteins; D007633:Keratins; D051096:Proto-Oncogene Proteins c-ret; D020794:Receptor Protein-Tyrosine Kinases; C416649:Ret protein, Drosophila