Activation of NF-kappaB and apoptosis of intestinal epithelial cells induced by hydrogen peroxide.
OBJECTIVE
In vitro model of hydrogen peroxide induced apoptosis of SW-480 cells was used to investigate the role of NF-kappaB in the pathogenesis of reactive oxygen species induced apoptosis of intestinal epithelial cells.


METHODS
Ultra-structural changes were observed. Apoptosis of SW-480 cell line was determined by Annexin-V and PI double-stained flow cytometry. Nuclear translocation of NF-kappaB was determined by anti-NF-kappaB polyclonal antibody and EB double-staining. NF-kappaB activity was studied by electrophoretic mobility shift assays. RT-PCR was performed to study expression of NF-kappaB mRNA.


RESULTS
Hydrogen peroxide led to apoptosis of SW-480 cells, condensed or semilunar chromatin even apoptotic bodies could be observed. Nuclear translocation of NF-kappaB, increase of NF-kappaB activity and expression of NF-kappaB mRNA were found simultaneously.


CONCLUSIONS
Early activation of NF-kappaB may be one of the mechanisms of apoptosis in intestinal epithelial cells by reactive oxygen species.
Li
Jianming
J
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Zhou
Hong
H
Cai
Qian
Q
Xiao
Guangxia
G
D017209:Apoptosis; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007413:Intestinal Mucosa / Q000378:metabolism*; D018613:Microscopy, Confocal; D016328:NF-kappa B / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000633:toxicity; D014407:Tumor Cells, Cultured
D016328:NF-kappa B; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide