title: Absence of donor-derived keratinocyte stem cells in skin tissues cultured from patients after mobilized peripheral blood hematopoietic stem cell transplantation.
abstract: OBJECTIVE
  Recent studies suggest that primitive bone marrow-derived cells contribute to regeneration of many tissues, including muscle, endothelium, myocardium, neural tissues, liver, and skin. Conversely, primitive cells resident in muscle and other tissues have been reported to reconstitute hematopoiesis. We investigated the contribution of cells with a primitive hematopoietic phenotype to human epidermal skin formation in recipients of allogeneic mobilized peripheral blood hematopoietic stem cell (HSC) transplantation.
  
  
  METHODS
  Our study population included female patients who had received granulocyte colony-stimulating factor mobilized peripheral blood HSC transplants from male donors for a variety of benign and malignant hematologic disorders at least 6 months before study entry, with a history of skin graft-vs-host disease. Epidermal skin cells (keratinocytes) obtained from punch biopsies of the skin were cultured under conditions specific for growth and expansion of homogenous populations of keratinocytes from keratinocyte stem cells. After multiple passages, DNA was extracted from cultured cells and evaluated by two different polymerase chain reaction (PCR) method for detection of Y chromosome specific sequences.
  
  
  RESULTS
  Neither sensitive PCR-based technique revealed the presence of male donor-derived keratinocyte stem cells in keratinocytes cultured from skin biopsies of female allogeneic transplantation recipients.
  
  
  CONCLUSIONS
  We could not confirm the contribution of donor mobilized peripheral blood hematopoietic stem cells to keratinocyte stem cell populations after HSC transplantation. These results cannot explain the presence of donor-derived cells with keratinocyte phenotypic markers in tissue sections of HSC transplant recipients.
authors.affiliation: Hematology Branch, NHLBI, National Institutes of Health, Bethesda, Md 20892, USA.
authors.forename: Peiman
authors.initials: P
authors.lastname: Hematti
authors.forename: Elaine M
authors.initials: EM
authors.lastname: Sloand
authors.forename: Cristian A
authors.initials: CA
authors.lastname: Carvallo
authors.forename: Michael R
authors.initials: MR
authors.lastname: Albert
authors.forename: Carole L
authors.initials: CL
authors.lastname: Yee
authors.forename: Monika M
authors.initials: MM
authors.lastname: Fuehrer
authors.forename: Jan K
authors.initials: JK
authors.lastname: Blancato
authors.forename: William G
authors.initials: WG
authors.lastname: Kearns
authors.forename: John A
authors.initials: JA
authors.lastname: Barrett
authors.forename: Richard W
authors.initials: RW
authors.lastname: Childs
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Vogel
authors.forename: Cynthia E
authors.initials: CE
authors.lastname: Dunbar
mesh_terms: D000328:Adult; D053523:Amelogenin; D001706:Biopsy; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D003746:Dental Enamel Proteins / Q000235:genetics; D000078404:Epidermal Cells; D005260:Female; D005500:Follow-Up Studies; D006085:Graft Survival; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015603:Keratinocytes / Q000166:cytology*; D017493:Leukocyte Common Antigens / Q000032:analysis; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009928:Organ Specificity; D013234:Stem Cells / Q000166:cytology*; D014019:Tissue Donors; D018183:Transplantation Chimera; D014184:Transplantation, Homologous; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics
chemical_list: D053523:Amelogenin; D003746:Dental Enamel Proteins; D016179:Granulocyte Colony-Stimulating Factor; D017493:Leukocyte Common Antigens