Functional study of Lactococcus lactis RNase III in Escherichia coli.
Endoribonuclease III (RNase III) is known to participate in a number of RNA maturation and decay pathways. Previous research carried out on citQRP mRNA processing had provided strong evidence that RNase III exists in Lactococcus lactis and acts as a major endoribonuclease in the control of citQRP mRNA stability. The DNA coding (rnc) for the putative lactococcal RNase III was cloned by PCR technology. In addition, we provide the transcriptional analysis of rnc gene and compared the lactococcal RNase III sequence to those of Gram-positive and Gram-negative organisms. This computer-assisted comparison showed an identity ranking from 37% to 54%, and the highest score was obtained with Streptococcus pneumoniae RNase III. Moreover, heterologous complementation indicated that lactococcal RNase III is able to complement the loss of Escherichia coli RNase III for both 30S RNA processing and lambda N posttranscriptional regulator.
Drider
Djamel
D
Laboratoire de Microbiologie Alimentaire et Industrielle, ENITIAA, rue de la Géraudière, BP 82225, F-44322 Nantes cedex 3, France. drider@enitiaa-nantes.fr
Bolotine
Alexander
A
Renault
Pierre
P
Prévost
Hervé
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004722:Endoribonucleases / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D012323:RNA Processing, Post-Transcriptional; D020871:RNA Stability; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D043244:Ribonuclease III; D017385:Sequence Homology; D017386:Sequence Homology, Amino Acid; D054334:Viral Regulatory and Accessory Proteins / Q000378:metabolism
D029968:Escherichia coli Proteins; C059417:N protein, Bacteriophage lambda; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D054334:Viral Regulatory and Accessory Proteins; D004722:Endoribonucleases; D043244:Ribonuclease III; C468366:ribonuclease III, E coli