title: SunghyangJungki-San Ga Pogongyoung inhibits IL-1 mediated tumour necrosis factor-alpha secretion in astrocytes.
abstract: Astrocytes play an important role in initiating and modulating inflammatory responses within the central nervous system (CNS). Extensive studies in rodents have shown that substance P induces inflammatory cytokine production in astrocytes. In this study we have examined whether an aqueous extract of SunghyangJungki-San Ga Pogongyoung (SSGP) inhibits the secretion of TNF-alpha from primary cultures of rat astrocytes. SSGP (10-1,000 microg/mL) significantly inhibited the TNF-alpha secretion by astrocytes stimulated with lipopolysaccharide (LPS) and substance P (SP). Interleukin-1 (IL-1) has been shown to elevate TNF-alpha secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore examined whether IL-1 mediated inhibition of TNF-alpha secretion from primary astrocytes by SSGP. Treatment with SSGP (10-1,000 microg/mL) to astrocytes stimulated with both LPS and SP decreased IL-1 secretion significantly. Moreover, the secretion of TNF-alpha by LPS and SP in astrocytes was progressively inhibited with an increasing amount of IL-1 neutralizing antibody. Our results suggest that SSGP may inhibit TNF-alpha secretion by inhibiting IL-1 secretion and that SSGP has an antiinflammatory activity in the CNS.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Ok-Youn
authors.initials: OY
authors.lastname: Lee
authors.forename: Hyung-Won
authors.initials: HW
authors.lastname: Kang
authors.forename: Ceon-Mok
authors.initials: CM
authors.lastname: Lee
authors.forename: Kyu-Sang
authors.initials: KS
authors.lastname: Lim
authors.forename: Kang-Chang
authors.initials: KC
authors.lastname: Lee
authors.forename: Seung-Taeck
authors.initials: ST
authors.lastname: Park
authors.forename: Seung Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Yeoung-Su
authors.initials: YS
authors.lastname: Lyu
authors.forename: Nyeon-Hyoung
authors.initials: NH
authors.lastname: An
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008518:Medicine, East Asian Traditional; D051379:Mice; D017628:Microglia / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D013373:Substance P / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C470019:sunghyangjungki-san ga pogongyoung; D013373:Substance P