title: Comparison of temporal changes in components of formalin guaiacol under several storage conditions.
abstract: This study examined the effects of storage conditions such as time course, temperature, fluorescent light, and darkness on the components and antibacterial activity of formalin guaiacol (FG) used in endodontic treatment. We measured the quantities of formaldehyde and guaiacol in FG and antibacterial activities against Staphylococcus aureus, Porphyromonas gingivalis, and Porphyromonas endodontalis. The components and antibacterial activity of FG in the brown or transparent tightly sealed containers were not affected by temperature or fluorescent light throughout the 4 week test. However, in the loosely sealed containers, formaldehyde and guaiacol in FG sample decreased remarkably within one week, not only in a temperature- and time-dependent manner, but also under fluorescent light at 20 degrees C. Furthermore, the antibacterial activities in the FG sample were significantly attenuated in parallel with the decrease in formaldehyde levels. Fluorescent light caused color changes and crystallization of FG samples in the transparent containers. These results suggest that it is important to replace fresh FG every 5 to 7 days for endodontic treatment and that, in the dental office, it is advisable to store fresh FG in tightly sealed containers every 2 weeks to maintain its efficacy.
authors.affiliation: Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yamano
authors.forename: Kohei
authors.initials: K
authors.lastname: Sawaki
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Ishihara
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ohno
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Kawaguchi
mesh_terms: D003116:Color; D003460:Crystallization; D003624:Darkness; D004202:Disinfectants / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects; D004338:Drug Combinations; D004356:Drug Storage; D005557:Formaldehyde / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects; D006139:Guaiacol / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects; D006801:Humans; D008027:Light; D008422:Materials Testing; D017861:Porphyromonas / Q000187:drug effects; D016966:Porphyromonas gingivalis / Q000187:drug effects; D012388:Root Canal Irrigants / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D004202:Disinfectants; D004338:Drug Combinations; D012388:Root Canal Irrigants; D005557:Formaldehyde; D006139:Guaiacol