Iron incorporation into Escherichia coli Dps gives rise to a ferritin-like microcrystalline core.
Escherichia coli Dps belongs to a family of bacterial stress-induced proteins to protect DNA from oxidative damage. It shares with Listeria innocua ferritin several structural features, such as the quaternary assemblage and the presence of an unusual ferroxidase center. Indeed, it was recently recognized to be able to oxidize and incorporate iron. Since ferritins are endowed with the unique capacity to direct iron deposition toward formation of a microcrystalline core, the structure of iron deposited in the E. coli Dps cavity was studied. Polarized single crystal absorption microspectrophotometry of iron-loaded Dps shows that iron ions are oriented. The spectral properties in the high spin 3d(5) configuration point to a crystal form with tetrahedral symmetry where the tetrahedron center is occupied by iron ions and the vertices by oxygen. Crystals of iron-loaded Dps also show that, as in mammalian ferritins, iron does not remain bound to the site after oxidation has taken place. The kinetics of the iron reduction/release process induced by dithionite were measured in the crystal and in solution. The reaction appears to have two phases, with t(12) of a few seconds and several minutes at neutral pH values, as in canonical ferritins. This behavior is attributed to a similar composition of the iron core.
Ilari
Andrea
A
C.N.R., Centro di Studio sulla Biologia Molecolare and Dipartimento di Scienze Biochimiche, A. Rossi Fanelli, Università di Roma La Sapienza, 00185 Roma, Italy.
Ceci
Pierpaolo
P
Ferrari
Davide
D
Rossi
Gian Luigi
GL
Chiancone
Emilia
E
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002570:Ceruloplasmin / Q000737:chemistry / Q000378:metabolism; D003460:Crystallization; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004227:Dithionite / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005293:Ferritins / Q000737:chemistry*; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D020836:Protein Structure, Quaternary; D013053:Spectrophotometry
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C083911:DPS protein, Bacteria; D004227:Dithionite; D005293:Ferritins; D007501:Iron; D002570:Ceruloplasmin