Expression of Fusion Protein Containing Androgen Receptor Segment and Preparation of Polyclonal Antibodies to Androgen Receptor.
The cDNA segment (1105-2224) of the androgen receptor was cloned into the pGEX-3X expression vector and expressed in E. coli. The soluble fusion proteins GST-AR were used to immunize rabbits to obtain polyclonal antibodies to androgen receptors. The antibodies were purified by GST-Sepharose 4B and indicated the specificity to androgen receptors. The purified antibodies can be used in study the function of AR.
Xie
Fang
F
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. yonglz@sunm.shcnc.ac.cn
Ping
Bei-Fang
BF
Zhang
Zu-Chuan
ZC
Zhang
Yong-Lian
YL