[Organization of the 378 bp satellite repeat in terminal heterochromatin of Allium fistulosum].
Telomeres, DNA-protein structures, are important elements of the eukaryotic chromosome. Telomeric regions of the majority of higher plants contain heptanucleotides TTTAGGG arranged into a tandem repeat. However, some taxa have no such repeats. These are some species of lilies (Lilium) and onions (Allium). For example, terminal regions of chromosomes of Spanish onion (Allium fistulosum) contain satellite DNA whose unit repeats are 380 bp in length, and the short arm of its chromosome 8 contains rDNA repeats. This study deals with the terminal heterochromatin and organization of the satellite repeat in A. fistulosum. Fluorescent in situ hybridization (FISH) was used to locate the satellite DNA on chromosomes and on extended DNA of A. fistulosum. Nonsatellite DNA was found in the structure of telomeric repeat. Polymerase chain reaction (PCR) and Southern hybridization were used for analysis of terminal heterochromatin. Various rearrangements were found in the satellite repeat. The roles of retrotransposones and microsatellites in the formation of terminal heterochromatin are discussed.
Fesenko
I A
IA
Timiryazev Agricultural Academy, Moscow, 127550 Russia.
Khrustaleva
L I
LI
Karlov
G I
GI
D000490:Allium / Q000235:genetics*; D015139:Blotting, Southern; D017931:DNA Primers; D004275:DNA, Ribosomal; D006570:Heterochromatin; D017404:In Situ Hybridization, Fluorescence; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction; D018626:Retroelements; D016615:Telomere / Q000235:genetics
D017931:DNA Primers; D004275:DNA, Ribosomal; D006570:Heterochromatin; D018626:Retroelements