title: Cloning, Sequencing and Expression of Human Copper, Zinc-superoxide Dismutase cDNA.
abstract: The cDNA encoding the human copper, zinc-superoxide dismutase(Cu, Zn-SOD) was amplified from the human liver by RT-PCR and sequenced. The cloned human Cu, Zn-SOD cDNA was ligated into expression vector pET-22b(+) under T7 promotor. After 3 h induction with 1 mmol/L IPTG, human Cu, Zn-SOD was highly expressed in E.coli BL21(DE3). The expression product was up to 30% of the total protein of the bacteria in soluble form, which had specific SOD activity.
authors.affiliation: Research Institute of Biochemistry, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. qsyuan@ecust.edu.cn
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Shi
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Fan
authors.forename: Dong-Zhi
authors.initials: DZ
authors.lastname: Wei
authors.forename: Qin-Sheng
authors.initials: QS
authors.lastname: Yuan
authors.forename: Jian-Xing
authors.initials: JX
authors.lastname: Sun
authors.forename: Xiang-Fu
authors.initials: XF
authors.lastname: Wu