Microstructure of anaerobic granules bioaugmented with Desulfitobacterium frappieri PCP-1.
Oligonucleotide probes were used to study the structure of anaerobic granular biofilm originating from a pentachlorophenol-fed upflow anaerobic sludge bed reactor augmented with Desulfitobacterium frappieri PCP-1. Fluorescence in situ hybridization demonstrated successful colonization of anaerobic granules by strain PCP-1. Scattered microcolonies of strain PCP-1 were detected on the biofilm surface after 3 weeks of reactor operation, and a dense outer layer of strain PCP-1 was observed after 9 weeks. Hybridization with probes specific for Eubacteria and Archaea probes showed that Eubacteria predominantly colonized the outer layer, while Archaea were observed in the granule interior. Mathematical simulations showed a distribution similar to that observed experimentally when using a specific growth rate of 2.2 day(-1) and a low bacterial diffusion of 10(-7) dm(2) day(-1). Also, the simulations showed that strain PCP-1 proliferation in the outer biofilm layer provided excellent protection of the biofilm from pentachlorophenol toxicity.
Lanthier
M
M
INRS-Institut Armand-Frappier, Laval, Québec, Canada H7V 1B7.
Tartakovsky
B
B
Villemur
R
R
DeLuca
G
G
Guiot
S R
SR
D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics; D001419:Bacteria / Q000235:genetics; D001673:Biodegradation, Environmental; D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D008954:Models, Biological; D015345:Oligonucleotide Probes; D010416:Pentachlorophenol / Q000378:metabolism*; D010459:Peptococcaceae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
D004275:DNA, Ribosomal; D015345:Oligonucleotide Probes; D012722:Sewage; D010416:Pentachlorophenol