DNA analysis of nosocomial infection by Enterobacter aerogenes in three cases of septicaemia in Japan.
Ceftazidime-resistant Enterobacter aerogenes was isolated from blood cultures of three patients with fever. DNA analysis using pulsed-field gel electrophoresis and ribosomal RNA gene restriction digest pattern analysis revealed that the strains were clonally similar to each other with a 79.3-96.0% homology. The same strain of E. aerogenes was isolated from a three-way stopcock connected to the indwelling catheter in one of the patients at a concentration of 45 cfu/mL. A similar strain was also isolated from the urine of one other patient on the same floor. The data suggest that E. aerogenes caused septicaemia via low bacterial contamination of a three-way stopcock in a peripheral drip intravenous infusion system in at least one patient, and that the outbreak of E. aerogenes infections was due to clonally-related strains.
Goshi
S
S
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Taneike
I
I
Nakagawa
S
S
Kojio
S
S
Tamura
Y
Y
Ohara
T
T
Ozaki
K
K
Tsukada
H
H
Aoki
Y
Y
Asakura
H
H
Gejyo
F
F
Itoh
M
M
Yamamoto
T
T
D000328:Adult; D003428:Cross Infection / Q000209:etiology*; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity; D004756:Enterobacteriaceae Infections / Q000209:etiology*; D004866:Equipment Contamination; D005260:Female; D006801:Humans; D007564:Japan; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D021521:Ribotyping / Q000379:methods*; D018805:Sepsis / Q000209:etiology*
D004269:DNA, Bacterial