title: Influences of Helicobacter pylori on gastric angiogenesis and ulcer healing in mice.
abstract: OBJECTIVE
  Helicobacter pylori infection is associated with peptic ulcers; however, it is unclear whether the bacterium delays ulcer healing. We investigated the influence of H. pylori on ulcer healing in mice. We also examined the influence of H. pylori infection on angiogenesis.
  
  
  METHODS
  An acetic acid ulcer was made in male BALB/c mice. Three days later (day 0), the animals were inoculated with H. pylori SS1 strain. The healing process of the ulcer was examined macroscopically and microscopically on days 0, 6 and 9. The index of angiogenesis was also determined using carmine dye injection.
  
  
  RESULTS
  On day 0, angiogenesis began at the ulcer margin while the mucosal epithelia had not yet regenerated. On days 6 and 9, angiogenesis and epithelial regeneration developed and ulcer size reduced. These phenomena were significantly suppressed in mice infected with H. pylori.
  
  
  CONCLUSIONS
  Helicobacter pylori infection significantly suppressed angiogenesis and delayed ulcer healing. These results indicate that H. pylori plays an important role in ulcer healing.
authors.affiliation: Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Japan.
authors.forename: Edhi
authors.initials: E
authors.lastname: Gunawan
authors.forename: Shingo
authors.initials: S
authors.lastname: Tsuji
authors.forename: Masahiko
authors.initials: M
authors.lastname: Tsujii
authors.forename: Arata
authors.initials: A
authors.lastname: Kimura
authors.forename: Wei-Hao
authors.initials: WH
authors.lastname: Sun
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sawaoka
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Sasayama
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Sunao
authors.initials: S
authors.lastname: Kawano
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Hori
mesh_terms: D019342:Acetic Acid / Q000633:toxicity; D000818:Animals; D005753:Gastric Mucosa / Q000098:blood supply*; D016481:Helicobacter Infections / Q000382:microbiology / Q000503:physiopathology*; D016480:Helicobacter pylori / Q000502:physiology*; D007202:Indicators and Reagents / Q000633:toxicity; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D018919:Neovascularization, Physiologic; D010437:Peptic Ulcer / Q000139:chemically induced / Q000382:microbiology / Q000503:physiopathology*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D007202:Indicators and Reagents; D019342:Acetic Acid