Microencapsulation of protein particles within lipids using a novel supercritical fluid process.
Solvent-free microparticles, loaded with a model protein (bovine serum albumin: BSA) have been produced using a novel coating process based on supercritical (SC) fluid technology. Coating material consists of either trimyristin (Dynasan114) or Gelucire50-02, two lipidic compounds having a high melting point. Microparticles were characterized as regards their morphology, protein content and in vitro release profile. A discontinuous coating made of crystalline micro-needles was obtained using Dynasan114. It led to particles with an initial burst release of about 70% in 30 min at 37 degrees C. However, a prolonged release of the protein has been achieved over a 24 h period from particles coated with Gelucire50-02, which produces a more homogeneous, film-forming coating. Furthermore, it was shown that BSA does not undergo any degradation after SCCO2 treatment under the SC conditions used in the coating process.
Ribeiro Dos Santos
I
I
Inserm ERIT-M 0104, Ingénierie de la vectorisation particulaire, 10 rue André Boquel, 49100, Angers, France.
Richard
J
J
Pech
B
B
Thies
C
C
Benoit
J P
JP
D002245:Carbon Dioxide / Q000737:chemistry; D003692:Delayed-Action Preparations; D004339:Drug Compounding / Q000379:methods*; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D005079:Excipients; D007700:Kinetics; D008055:Lipids / Q000737:chemistry; D010316:Particle Size; D011312:Pressure; D011506:Proteins / Q000008:administration & dosage / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012997:Solvents; D013499:Surface Properties
D003692:Delayed-Action Preparations; D005079:Excipients; D008055:Lipids; D011506:Proteins; D012997:Solvents; D002245:Carbon Dioxide; D012710:Serum Albumin, Bovine