BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling.
Brassinosteroids regulate plant growth and development through a protein complex that includes the leucine-rich repeat receptor-like protein kinase (LRR-RLK) brassinosteroid-insensitive 1 (BRI1). Activation tagging was used to identify a dominant genetic suppressor of bri1, bak1-1D (bri1-associated receptor kinase 1-1Dominant), which encodes an LRR-RLK, distinct from BRI1. Overexpression of BAK1 results in elongated organ phenotypes, while a null allele of BAK1 displays a semidwarfed phenotype and has reduced sensitivity to brassinosteroids (BRs). BAK1 is a serine/threonine protein kinase, and BRI1 and BAK1 interact in vitro and in vivo. Expression of a dominant-negative mutant allele of BAK1 causes a severe dwarf phenotype, resembling the phenotype of null bri1 alleles. These results indicate BAK1 is a component of BR signaling.
Li
Jia
J
Division of Biological Sciences, University of Missouri-Columbia, Columbia, MO 65211, USA.
Wen
Jiangqi
J
Lease
Kevin A
KA
Doke
Jason T
JT
Tax
Frans E
FE
Walker
John C
JC
D000483:Alleles; D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D060406:Brassinosteroids; D002777:Cholestanols / Q000494:pharmacology; D004789:Enzyme Activation; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D006914:Hydroxysteroids / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D013260:Steroids, Heterocyclic / Q000494:pharmacology; D014018:Tissue Distribution
D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D002777:Cholestanols; D006914:Hydroxysteroids; D008565:Membrane Proteins; D012097:Repressor Proteins; D013260:Steroids, Heterocyclic; D011494:Protein Kinases; C463660:BAK1 protein, Arabidopsis; C108270:BRI1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; C023623:brassinolide