PDCD2 is a negative regulator of HCF-1 (C1).
Temperature sensitive mutations in host cell factor 1 (HCF-1) arrest cells in the middle of the G1 phase of the cycle. We have shown that the highly conserved C-terminal WYF domain of HCF-1 protein interacts with the MYND domain of the PDCD2 protein. This inter-action is conserved between human HCF-1 and HCF-2 and the C. elegans HCF. Overexpression of PDCD2, which interacts with the N-CoR/mSin3A corepressor complexes, suppresses cotransfected HCF-1 complement-ation of a temperature lesion in the endogenous HCF-1 protein. Overexpression of domains of either PDCD2 or HCF-1, which should interfere with interactions between these two proteins, enhances the complementation.
Scarr
Rebecca B
RB
Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts, MA 02139-4307, USA.
Sharp
Phillip A
PA
D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001665:Binding Sites; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins; D002478:Cells, Cultured; D017353:Gene Deletion; D005816:Genetic Complementation Test; D051863:Host Cell Factor C1; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D056971:Nuclear Receptor Co-Repressor 1; D011233:Precipitin Tests; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D020798:Two-Hybrid System Techniques
D051017:Apoptosis Regulatory Proteins; D029742:Caenorhabditis elegans Proteins; C497692:HCFC1 protein, human; D051863:Host Cell Factor C1; C539127:NCOR1 protein, human; D009687:Nuclear Proteins; D056971:Nuclear Receptor Co-Repressor 1; C094601:PDCD2 protein, human; D011506:Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C497691:hcf-1 protein, C elegans