title: Deep-etched view of the cuticle of adults of Litomosoides chagasfilhoi (Nematoda: Filarioidea).
abstract: The fine structure of the cuticle of the filariid nematode Litomosoides chagasfilhoi is described, based on observations made by transmission electron microscopy and deep-etched replicas. The cuticle consists of a trilaminate epicuticle, the outermost layer that interfaces with the host and four other layers: cortical, intermediate, fibrous and basal. In deep-etched replicas, the cortical layer is formed by a meshwork of globular particles and fibers with a thickness of 4-8 nm. The intermediate layer is electron-lucid and contains a densely-stained line. In deep-etched replicas, it is composed by a meshwork of fibers with longitudinal orientation. The fibrous layer is the thickest and most electron-dense and consists of two types of fibers: thick (9-26 nm) and thin (3-6 nm). The innermost basal layer is intimately associated with the hypodermis. In common with other nematodes, the cuticle of L. chagasfilhoi presents channels that are probably involved in nutrient acquisition and transport.
authors.affiliation: Laboratório de Helmintologia, Programa de Biologia Celular e Parasitologia, IBCCF, UFRJ, Cidade Universitária, Rio de Janeiro, Brazil.
authors.forename: A H A
authors.initials: AH
authors.lastname: de Moraes Neto
authors.forename: R M
authors.initials: RM
authors.lastname: Lanfredi
authors.forename: W
authors.initials: W
authors.lastname: de Souza
mesh_terms: D000818:Animals; D005370:Filarioidea / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure*; D005613:Freeze Etching / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods