Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli.
We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.
Chin
Jason W
JW
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Santoro
Stephen W
SW
Martin
Andrew B
AB
King
David S
DS
Wang
Lei
L
Schultz
Peter G
PG
D001386:Azides / Q000378:metabolism*; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D003432:Cross-Linking Reagents; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D017017:Methanococcus / Q000201:enzymology; D009211:Myoglobin / Q000096:biosynthesis; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D014448:Tyrosine-tRNA Ligase / Q000378:metabolism
D001386:Azides; D003432:Cross-Linking Reagents; D004269:DNA, Bacterial; D009211:Myoglobin; C027162:4-azidophenylalanine; D010649:Phenylalanine; D015500:Chloramphenicol O-Acetyltransferase; D014448:Tyrosine-tRNA Ligase