Production of Anti-P16 Sera by Genetic Immunization and Protein Immunization.
Genetic immmunization is a new method of producing antibody, which is different from protein immunization. In order to produce anti-P16 sera and to find out the difference between genetic immunization and protein immunization, fusion protein GST-P16 and eukaryotic expression vector pCMV-p16 were injected in rabbit respectively. Western blot analysis showed that the titer of sera from protein immunization was 1:625, which was much higher than the titer from genetic immunization sera. Our results indicate that much has to be done on the characteristics of the genetic immunization and methods for enhancing its immunization effect, before we can use it widely to produce antibody.
Qin
Lin-Hua
LH
Military Key Laboratory of Medical Molecular Genetics, Second Military Medical University, Shanghai 200433, China.
Huang
Chang-Hui
CH
Fu
Ji-Liang
JL