The importance of manganese superoxide dismutase in delayed preconditioning: involvement of reactive oxygen species and cytokines.
It is clinically important to elucidate the mechanism underlying the delayed preconditioning against ischemia-reperfusion injury observed 24-72 h after sublethal stress such as brief ischemia, hyperthermia and exercise. The time course of induction of myocardial manganese-superoxide dismutase (Mn-SOD) and appearance of the ischemic tolerance coincide well, and the percent increase in Mn-SOD activity and percent reduction of infarct size are correlated well under various stresses. Furthermore, treatments with antisense oligodeoxynucleotides to Mn-SOD completely abolished the delayed preconditioning and any increase in Mn-SOD content. These results indicate that Mn-SOD induction plays a pivotal role in the late phase preconditioning afforded with brief ischemia, hyperthermia and exercise. We also showed that cytokines, e.g., tumor necrosis factor-alpha and interleukin-1beta, and reactive oxygen species are involved in the process of signal transduction for the Mn-SOD induction.
Hoshida
Shiro
S
Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Suita, Japan. hoshidas@orh.go.jp
Yamashita
Nobushige
N
Otsu
Kinya
K
Hori
Masatsugu
M
D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D016373:DNA, Antisense / Q000494:pharmacology; D004789:Enzyme Activation; D015444:Exercise / Q000502:physiology; D005334:Fever / Q000378:metabolism; D006801:Humans; D019157:Ischemic Preconditioning, Myocardial; D017202:Myocardial Ischemia / Q000276:immunology / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D016207:Cytokines; D016373:DNA, Antisense; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase