title: Large-scale proteomic analysis of the human spliceosome.
abstract: In a previous proteomic study of the human spliceosome, we identified 42 spliceosome-associated factors, including 19 novel ones. Using enhanced mass spectrometric tools and improved databases, we now report identification of 311 proteins that copurify with splicing complexes assembled on two separate pre-mRNAs. All known essential human splicing factors were found, and 96 novel proteins were identified, of which 55 contain domains directly linking them to functions in splicing/RNA processing. We also detected 20 proteins related to transcription, which indicates a direct connection between this process and splicing. This investigation provides the most detailed inventory of human spliceosome-associated factors to date, and the data indicate a number of interesting links coordinating splicing with other steps in the gene expression pathway.
authors.affiliation: Protein Interaction Laboratory in the Center of Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark.
authors.forename: Juri
authors.initials: J
authors.lastname: Rappsilber
authors.forename: Ursula
authors.initials: U
authors.lastname: Ryder
authors.forename: Angus I
authors.initials: AI
authors.lastname: Lamond
authors.forename: Matthias
authors.initials: M
authors.lastname: Mann
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019295:Computational Biology / Q000379:methods; D005786:Gene Expression Regulation; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics; D020543:Proteome / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing / Q000235:genetics; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics; D015398:Signal Transduction; D017381:Spliceosomes / Q000737:chemistry* / Q000235:genetics; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics
chemical_list: D009687:Nuclear Proteins; D020543:Proteome; D012269:Ribosomal Proteins; D014157:Transcription Factors