[Study on the expression of E2 gene of classical swine fever virus in Pichia pastoris and the immunological activity of its expression product].
E2 gene of classical swine fever virus (CSFV) was cloned into secretory pPIC9K Pichia pastoris expression vector. After being linearized by digestion, the vector was transformed into Pichia pastoris by electroporation to integrate with the genome, the transformants with high copies were screened by G418 and were induced to express with methonal. The results of SDS-PAGE and Western blot demonstrated that the supernatant of the induced P. pastoris culture contained protein E2. The results of the study on the immunological activity indicated that the protein E2 expressed in P. pastoris can elicit animal bodies to produce antibodies against protein E2.
Han
Xue-Qing
XQ
Lanzhou Veterinary Research Institute, CAS, Lanzhou 730046, China. hanxq8@hotmail.com
Liu
Xiang-Tao
XT
Zhang
Yong
Y
Xie
Qing-Ge
QG
Tian
Bo
B
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D015870:Gene Expression; D010843:Pichia; D011817:Rabbits; D013552:Swine; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus