Hypothermia inhibits cell proliferation and nitric oxide synthase expression in rats.
In the present study, the effects of cold-water immersion on cell proliferation and nitric oxide synthase expression in the dentate gyrus of rats were investigated. Sprague-Dawley rats were divided into four groups: the control-rest group; the control-heat group; the cold-rest group; and the cold-heat group. Cold-water immersion for 5 min at 4 degrees C suppressed the numbers of 5-bromo-2'-deoxyuridine-positive and nicotinamide adenine dinucleotide phosphate-diaphorase-positive cells in the dentate gyrus, and these numbers were increased by warming for 30 min at 30 degrees C. In the present study, it was demonstrated that warming protects against cold stress-induced suppression of new cell formation, and results suggest that nitric oxide, the synthesis of which is affected adversely by cold-water immersion, may play an important role in the regulation of cell proliferation.
Lee
Kwang-Sik
KS
Department of Physiology, College of Medicine, Kyung Hee University, 1 Hoigi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
Lim
Baek-Vin
BV
Jang
Mi-Hyeon
MH
Shin
Min-Chul
MC
Lee
Taeck-Hyun
TH
Kim
Young-Pyo
YP
Shin
Ho-Su
HS
Cho
Seong-Yeon
SY
Kim
Hong
H
Shin
Mal-Soon
MS
Kim
Ee-Hwa
EH
Kim
Chang-Ju
CJ
D000818:Animals; D000963:Antimetabolites / Q000032:analysis; D001973:Bromodeoxyuridine / Q000032:analysis; D002455:Cell Division / Q000502:physiology; D003080:Cold Temperature; D018891:Dentate Gyrus / Q000166:cytology* / Q000201:enzymology; D006358:Hot Temperature; D016857:Hypocapnia / Q000503:physiopathology; D007035:Hypothermia / Q000503:physiopathology*; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012018:Reflex / Q000502:physiology; D013312:Stress, Physiological / Q000503:physiopathology; D014661:Vasoconstriction / Q000502:physiology; D014867:Water
D000963:Antimetabolites; D014867:Water; D019001:Nitric Oxide Synthase; D001973:Bromodeoxyuridine