60Co gamma irradiation prevents Bothrops jararacussu venom neurotoxicity and myotoxicity in isolated mouse neuromuscular junction.
The ability of gamma radiation from 60Co (2000 Gy) to attenuate the toxic effects of Bothrops jararacussu venom was investigated on mouse neuromuscular preparations in vitro. A comparative study between the effects of native and irradiated venoms was performed on both phrenic--diaphragm (PD) and extensor digitorum longus (EDL) preparations by means of myographic, biochemical and morphological techniques. Native venom (10 and 20 micro g/ml) induced a concentration--dependent paralysis of both directly and indirectly evoked contractions on PD preparations. At 20 micro g /ml, it also caused a pronounced myotoxic effect on the EDL muscle preparation that was characterized by an increase of creatine kinase release and by several morphological changes of this preparation. By contrast, irradiated venom, even at concentrations as high as 40 micro g/ml, induced neither paralyzing nor myotoxic effects. It was concluded that 60Co gamma radiation is able to abolish both the paralyzing and the myotoxic effects of B. jararacussu venom on the mouse neuromuscular junction. These findings support the hypothesis that gamma radiation could be an important tool to improve antisera production by reducing toxicity while preserving immunogenicity.
Souza
Fábio A D
FA
Departamento de Farmacologia, Instituto de Biociências, Universidade Estadual Paulista, Rubião Júnior, Botucatu, CEP 18618-000, São Paulo, Brazil.
Spencer
Patrick J
PJ
Rogero
José R
JR
Nascimento
Nanci
N
Dal Pai-Silva
Maeli
M
Gallacci
Márcia
M
D000818:Animals; D017837:Bothrops; D003037:Cobalt Radioisotopes; D003402:Creatine Kinase / Q000378:metabolism; D003435:Crotalid Venoms / Q000528:radiation effects* / Q000633:toxicity*; D004576:Electromyography; D005720:Gamma Rays; D008297:Male; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000473:pathology; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D010243:Paralysis / Q000139:chemically induced
D003037:Cobalt Radioisotopes; D003435:Crotalid Venoms; D003402:Creatine Kinase