title: The NorR protein of Escherichia coli activates expression of the flavorubredoxin gene norV in response to reactive nitrogen species.
abstract: The Escherichia coli norVW genes encode a flavorubredoxin and NADH:(flavo)rubredoxin reductase, respectively, which are involved in nitric oxide detoxification under anaerobic growth conditions. Here it is shown that the norVW genes also have a role in protection against reactive nitrogen intermediates generated from nitroprusside. Transcription from the norV promoter is activated by the presence of nitroprusside in the growth medium; activation requires the product of a divergently transcribed regulatory gene, norR.
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom.
authors.forename: Matthew I
authors.initials: MI
authors.lastname: Hutchings
authors.forename: Neeraj
authors.initials: N
authors.lastname: Mandhana
authors.forename: Stephen
authors.initials: S
authors.lastname: Spiro
mesh_terms: D001483:Base Sequence; D021901:DNA, Intergenic / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010641:Phenotype; D011401:Promoter Regions, Genetic / Q000235:genetics; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D021901:DNA, Intergenic; D029968:Escherichia coli Proteins; D026361:Reactive Nitrogen Species; D014157:Transcription Factors; C451400:YgaK protein, E coli; D009569:Nitric Oxide; C023091:rubredoxin-NAD+ reductase; D009247:NADH, NADPH Oxidoreductases