title: Linkage map organization of expressed sequence tags and sequence tagged sites in the mosquito, Aedes aegypti.
abstract: A composite genetic linkage map for the yellow fever mosquito Aedes aegypti was constructed based on restriction fragment length polymorphism (RFLP), single nucleotide polymorphism (SNP) and single strand conformation polymorphism (SSCP) markers. The map consists of 146 marker loci distributed across 205 cM, and includes several morphological mutant marker loci. Most of the genetic markers are derived from random cDNAs or Ae. aegypti genes of known function. A number of markers are derived from random genomic DNAs, including several cloned RAPD-PCR fragments, and also several cDNAs from Drosophila melanogaster. Most of the random cDNAs (80.2%) have high BlastX sequence identities to known genes, with the majority of matches to genes from D. melanogaster. Access to sequence data for all markers will facilitate their continued development for use in high-throughput SNP marker analyses and also provides additional physical anchor points for an anticipated genome sequencing effort.
authors.affiliation: Center for Tropical Disease Research and Training, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA. David.W.Severson.1@nd.edu
authors.forename: D W
authors.initials: DW
authors.lastname: Severson
authors.forename: J K
authors.initials: JK
authors.lastname: Meece
authors.forename: D D
authors.initials: DD
authors.lastname: Lovin
authors.forename: G
authors.initials: G
authors.lastname: Saha
authors.forename: I
authors.initials: I
authors.lastname: Morlais
mesh_terms: D000330:Aedes / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library; D005819:Genetic Markers; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers; D004247:DNA