title: Expression of Fusion Protein Containing Androgen Receptor Segment and Preparation of Polyclonal Antibodies to Androgen Receptor.
abstract: The cDNA segment (1105-2224) of the androgen receptor was cloned into the pGEX-3X expression vector and expressed in E. coli. The soluble fusion proteins GST-AR were used to immunize rabbits to obtain polyclonal antibodies to androgen receptors. The antibodies were purified by GST-Sepharose 4B and indicated the specificity to androgen receptors. The purified antibodies can be used in study the function of AR.
authors.affiliation: Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. yonglz@sunm.shcnc.ac.cn
authors.forename: Fang
authors.initials: F
authors.lastname: Xie
authors.forename: Bei-Fang
authors.initials: BF
authors.lastname: Ping
authors.forename: Zu-Chuan
authors.initials: ZC
authors.lastname: Zhang
authors.forename: Yong-Lian
authors.initials: YL
authors.lastname: Zhang