Correlation of Calcium-independent Phosphatidylcholine-specific Phospholipase C with gamma-GT in Hepatoma Cells.
We studied the activities of calcium-independent phosphatidylcholine-specific phospholipase C (PC-PLC) and the relationship between PC-PLC and gamma-GT in hepatoma cells. We noted that PC-PLC activity decreased significantly during hepatocarcinogenesis and the proliferation of CBRH-7919 cells, but increased significantly during differentiation. There was a close relationship between PC-PLC and gamma-GT activities. When CBRH-7919 cells were treated with exogenous PC-PLC, it took only 15 minutes for gamma-GT activity to decrease significantly and to the lowest level at 24 hours. However, gamma-GT activity in culture medium increased within 2 hours, then decreased afterward. We conclude that PC-PLC may regulate gamma-GT via certain pathways.
Wu
Xing-Zhong
XZ
Department of Biochemistry, Shanghai Medical University, Shanghai 200032, China.
Lu
Hong
H