Cytotoxic effects of macrophages and asbestos on transformed rat mesothelial cells.
Asbestos produced a cytotoxic effect on transformed cells of rat pleural mesothelium and on IAR2 epithelial cells and Rat1 fibroblasts transformed by ras oncogene, but not on normal cells of these strains under conditions of coculturing with peritoneal macrophages. Contact of mesothelioma cells, but not macrophages with asbestos was necessary and sufficient for attaining the cytotoxic effect. Macrophage-conditioned medium potentiated asbestos cytotoxicity for transformed mesothelial cells, but not for IARS-ras and Rat1-ras.
Furalev
V A
VA
Laboratory of Natural Carcinogens, Institute of Carcinogenesis, N. N. Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow.
Kravchenko
I V
IV
Vasil'eva
L A
LA
Pylev
L N
LN
D000818:Animals; D001194:Asbestos / Q000009:adverse effects*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D018920:Coculture Techniques; D003470:Culture Media; D011905:Genes, ras; D006801:Humans; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D018301:Neoplasms, Mesothelial / Q000209:etiology / Q000473:pathology*; D010994:Pleura / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D003470:Culture Media; D001194:Asbestos