title: Direct wiring of cytochrome c's heme unit to an electrode: electrochemical studies.
abstract: A novel strategy for the immobilization of cytochrome c on the surface of chemically modified electrodes is demonstrated and used to investigate the protein's electron-transfer kinetics. Mixed monolayer films of alkanethiols and omega-terminated alkanethiols (terminated with pyridine, imidazole, or nitrile groups that are able to ligate with the heme) are used to adsorb cytochrome c to the surface of gold electrodes. The use of mixed films, as opposed to pure films, allows the concentration of adsorbed cytochrome to remain dilute and ensures a higher degree of homogeneity in their environment. The adsorbed protein is studied using electrochemical methods and scanning tunneling microscopy.
authors.affiliation: Contribution from the Chemistry Department, University of Pittsburgh, Pennsylvania 15260, USA.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wei
authors.forename: Haiying
authors.initials: H
authors.lastname: Liu
authors.forename: Allison R
authors.initials: AR
authors.lastname: Dick
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Yufan
authors.initials: Y
authors.lastname: He
authors.forename: David H
authors.initials: DH
authors.lastname: Waldeck
mesh_terms: D003574:Cytochrome c Group / Q000737:chemistry*; D004563:Electrochemistry; D006418:Heme / Q000737:chemistry*
chemical_list: D003574:Cytochrome c Group; D006418:Heme