Analysis of Monosaccharides Derivatized with 2-aminoacridone by Capillary Electrophoresis.
A new method for simultaneous analysis of 11 monosaccharides with reducing ends derivatized with 2-aminoacridone (AMAC) by the capillary electrophoresis in 300 mmol/L borate, pH 10.5 or 100 mmol/L disodium tetraborate and a pH 10.5 buffer was described. The chemical derivatization was performed at 45 degrees for 5 h, and then the derivatized monosaccharides were separated by capillary electrophoresis and detected at 264 nm. The chemical derivatization limit was 10 &mgr;mol/L. Concentration and mass detection limits of 0.6 &mgr;mol/L and 17 fmol, respectively, could be achieved. The linear correlation coefficients between the quotient of each integrated peak area divided by retention time and the concentration of monosaccharide were all greater than 0.992 (n=6) for the 11 monosaccharides in concentration ranging from 5 to 25 &mgr;mol/L. The relationship between the electrophoretic mobility of the derivatives and the structure of sugar moieties was discussed. The carbohydrate components of several glycoproteins were also by the method described above.
Che
Fa-Yun
FY
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. xiaqc@sunm.shcnc.ac.cn
Liu
Zheng-Yu
ZY
Wang
Ke-Yi
KY
Xia
Qi-Chang
QC