The propagation of a porcine epidemic diarrhea virus in swine cell lines.
A strain of porcine epidemic diarrhea virus (PEDV), P-5V, utilized as a live virus vaccine in Japan was infected to a swine cell lines, KSEK6 and IB-RS-2 cells. Clear CPE, characterized by cellular destruction, started to appear in the infected cells on 2-3 days post infection (DPI) and affected cells was completely degenerated on 4 DPI. The virus was serially passaged in the cells even without addition of trypsin. Small but clear plaques were formed under an agar overlay medium on the cells. The infective titer in the order of 10(7.00-7.50) TCID50 per ml was obtained at usual incubation temperature.
Kadoi
K
K
Laboratory of Bio-medical Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
Sugioka
H
H
Satoh
T
T
Kadoi
B K
BK
D000818:Animals; D002478:Cells, Cultured; D003332:Coronaviridae / Q000254:growth & development* / Q000276:immunology; D003333:Coronaviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D003588:Cytopathogenic Effect, Viral / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology*; D010948:Viral Plaque Assay / Q000662:veterinary; D014765:Viral Vaccines / Q000276:immunology
D014765:Viral Vaccines