Effect of beta-amyloid peptide fragment 25-35 on nonselective permeability of mitochondria.
Beta-amyloid peptide fragment 25-35 potentiated phosphate- and calcium-induced opening of mitochondrial channels and caused swelling of mitochondria (even without exogenous calcium and phosphate). These changes were accompanied by accumulation of lipid peroxidation products in mitochondria. Specific inhibitors of mitochondrial channels ADP and cyclosporine A prevented beta-amyloid peptide-induced swelling of mitochondria. Our findings suggest that potentiation of mitochondrial channel opening is an important component of the neurodegenerative effect of beta-amyloid.
Shevtzova
E F
EF
Laboratory of Neurochemistry, Institute of Physiologically Active Substances, Russian Academy of Sciences, Chernogolovka. shevt@ipac.ac.ru
Kireeva
E G
EG
Bachurin
S O
SO
D016229:Amyloid beta-Peptides / Q000737:chemistry*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D003574:Cytochrome c Group / Q000096:biosynthesis; D004298:Dopamine / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D009474:Neurons / Q000473:pathology; D009638:Norepinephrine / Q000096:biosynthesis; D010446:Peptide Fragments / Q000737:chemistry*; D019289:Pyruvic Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D016229:Amyloid beta-Peptides; D003574:Cytochrome c Group; D007773:Lactates; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); D016572:Cyclosporine; D019289:Pyruvic Acid; D002118:Calcium; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine