title: Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli.
abstract: We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.
authors.affiliation: Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Jason W
authors.initials: JW
authors.lastname: Chin
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Santoro
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Martin
authors.forename: David S
authors.initials: DS
authors.lastname: King
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Peter G
authors.initials: PG
authors.lastname: Schultz
mesh_terms: D001386:Azides / Q000378:metabolism*; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D003432:Cross-Linking Reagents; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D017017:Methanococcus / Q000201:enzymology; D009211:Myoglobin / Q000096:biosynthesis; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D014448:Tyrosine-tRNA Ligase / Q000378:metabolism
chemical_list: D001386:Azides; D003432:Cross-Linking Reagents; D004269:DNA, Bacterial; D009211:Myoglobin; C027162:4-azidophenylalanine; D010649:Phenylalanine; D015500:Chloramphenicol O-Acetyltransferase; D014448:Tyrosine-tRNA Ligase