Dual Regulation of Tyrosine Protein Kinase and Protein Kinase C on N-acetylglucosaminyltransferase V.
The effects of the epidermal growth factor (EGF), a stimulator of tyrosine protein kinase (TPK), and phorblol-12-myristate-13-acetate (PMA), a stimulator of protein kinase C (PKC), on the activity of N-acetylglucosaminyltransferase V (GnT-V) were studied in human hepatocarcinoma cell line 7721 in order to elucidate the regulation of TPK and PKC on GnT-V. It was found that the GnT-V activity obviously increased after treatment of the cells with EGF or PMA for 48 h. A non-specific protein kinase inhibitor, quercetin, inhibited the activities of TPK and PKC(inhibited mainly the membranous TPK and PKC)as well as GnT-V simulatanously. Moreover, quercetin completely eliminated the stimulating effect of EGF or PMA on GnT-V. When Tyrohostin-25, a specific inhibitor of TPK, or sphingosine, the specific inhibitor of PKC, was used separately to substitute for quercetin, the induction effect of EGF of PMA on GnT-V was only partially eliminated. However, when both Tyrphostin-25 and sphingosine were added to the culture medium, the elevation of GnT-V caused by EGF or PMA was entirely blocked. Cycloheximide, a well-known inhibitor of protein synthesis, showed an effect similar to the inhibition of protein kinases;it not only inhibited the basal activity of GnT-V, but also abolished the inducing stimulation of GnT-V by EGF of PMA. These results indicate that EGF or PMA regulates the activity of GnT-V via protein kinases, and GnT-V is regulated by dual mechanism of membranous TPK and PKC. Membranous TPK is more important that membranous PKC in the regulation of GnT-V.
Xia
Bao-Yun
BY
Key Laboratory of Glycoconjugate Research, Ministry of Public Health, Department of Biochemistry, Shanghai Medical University, Shanghai 200032, China. hlchen@shmu.edu.cn
Guo
Hua-Bei
HB
Chen
Hui-Li
HL