title: Alveolar echinococcosis: characterization of diagnostic antigen Em18 and serological evaluation of recombinant Em18.
abstract: The Echinococcus multilocularis protein Em18 is one of the most promising antigens for use in serodiagnosis of alveolar echinococcosis in human patients. Here we identify an antigenic relationship between Em18 and a 65-kDa immunodominant E. multilocularis surface protein previously identified as either EM10 or EmII/3. The NH(2)-terminal sequence of native Em18 was determined, revealing it to be a fragment of EM10. Experiments were undertaken to investigate the effect of proteinase inhibitors on the degradation of EM10 in crude extracts of E. multilocularis protoscoleces. Em18 was found to be the product of degradation of EM10 by cysteine proteinase. A recombinant Em18 (RecEm18, derived from (349)K to (508)K of EM10) was successfully expressed by using Escherichia coli expression system and then evaluated for use in serodiagnosis of alveolar echinococcosis. RecEm18 was recognized by 27 (87.1%) and 28 (90.3%) of 31 serum samples from clinically and/or pathologically confirmed alveolar echinococcosis patients by enzyme-linked immunosorbent assay and immunoblotting, respectively. Of 33 serum samples from cystic echinococcosis patients, 1 was recorded as having a weak positive reaction to RecEm18; however, none of the serum samples which were tested from neurocysticercosis patients (n = 10) or healthy people (n = 15) showed positive reactions. RecEm18 has the potential for use in the differential serodiagnosis of alveolar echinococcosis.
authors.affiliation: Department of Parasitology, Asahikawa Medical College, Japan. yasusako@asahikawa-med.ac.jp
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sako
authors.forename: Minoru
authors.initials: M
authors.lastname: Nakao
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakaya
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasaki
authors.forename: Bruno
authors.initials: B
authors.lastname: Gottstein
authors.forename: Marshall W
authors.initials: MW
authors.lastname: Lightowers
authors.forename: Peter M
authors.initials: PM
authors.lastname: Schantz
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology; D004444:Echinococcosis, Hepatic / Q000175:diagnosis*; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C117889:Em18 antigen; D011994:Recombinant Proteins