Studies on Site-specific DNA Excision and Targeted DNA Integration in Saccharomyces cerevisiae.
The FLP-FRT site-specific recombination system in the yeast Saccharomyces cerevisiae consists of two components: FLP recombinase and FRT site. The FLP gene of the yeast 2 &mgr;m circle was placed under control of GAL10 promoter. As a result of galactose induction, the DNA sequence between two FRT direct repeats was excised by expressed FLP recombinase. Moreover, a circular plasmid containing a FRT site was site-specifically integrated into chromosome via site-specific recombination with a preset FRT site on the chromosome. The targeted DNA excision and integration events catalyzed by FLP recombinase are precise and very efficient.
Gao
Xiang-Dong
XD
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China. yyli@fudan.edu.cn
Li
Yu-Yang
YY