Identification of Streptococcus bovis biotype I strains among S. bovis clinical isolates by PCR.
Streptococcus bovis causes 24% of all streptococcal infective endocarditis cases. There are many reports linking both S. bovis bacteremia and endocarditis with various forms of gastrointestinal disease (primarily colonic cancers). S. bovis is divided into two biotypes: I and II. The biotype I strain is much more frequently isolated from patients with endocarditis, gastrointestinal disease, or both. We describe here the isolation of biotype I-specific DNA sequences and the development of a PCR test which can identify S. bovis biotype I strains among S. bovis clinical isolates.
Songy
Wanda B
WB
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305, USA.
Ruoff
Kathryn L
KL
Facklam
Richard R
RR
Ferraro
Mary J
MJ
Falkow
Stanley
S
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D013290:Streptococcal Infections / Q000382:microbiology; D016985:Streptococcus bovis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D001426:Bacterial Proteins; D004269:DNA, Bacterial