title: Self-regulation of rat liver GAP junction by phosphorylation.
abstract: We have studied the functioning of rat liver Connexin 32 (C x 32) at the single channel level in presence of ATP. It was observed that ATP regulates the functioning of the channel by running down the junctional conductance. A non-specific exogenous protein phosphatase (alkaline phosphatase) reversed the rundown of junctional activity to its normal functioning state. Autoradiograhic studies demonstrate autophosphorylation of rat liver C x 32. These findings indicate a self-regulatory mechanism of the channel.
authors.affiliation: Department of Biophysics, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021,India.
authors.forename: Paramita
authors.initials: P
authors.lastname: Ghosh
authors.forename: Subhendu
authors.initials: S
authors.lastname: Ghosh
authors.forename: Sudipto
authors.initials: S
authors.lastname: Das
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D017630:Connexins / Q000737:chemistry / Q000378:metabolism*; D015536:Down-Regulation; D004553:Electric Conductivity; D017629:Gap Junctions / Q000737:chemistry / Q000378:metabolism*; D066298:In Vitro Techniques; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D010766:Phosphorylation; D011510:Proteolipids; D051381:Rats; D000097002:Gap Junction beta-1 Protein
chemical_list: D000255:Adenosine Triphosphate; D017630:Connexins; D008051:Lipid Bilayers; D011510:Proteolipids; D000097002:Gap Junction beta-1 Protein; C020485:proteoliposomes