Applications of CpG island microarrays for high-throughput analysis of DNA methylation.
Differential methylation hybridization (DMH) is a high-throughput microarray technique designed to identify changes in DNA methylation patterns commonly observed in cancer and other disease states. The DMH methodology comprises three fundamental components: the arraying of CpG island clones on glass slides, the preparation of the sample amplicons under investigation, and the hybridization of amplicon targets onto the CpG island microarray. Herein, we outline the DMH protocol and illustrate its utility and the validation approaches used in analyzing the hypermethylation profile of breast tumor and normal samples.
Yan
Pearlly S
PS
Department of Pathology and Anatomical Sciences, Ellis Fischel Cancer Center, University of Missouri, Columbia 65203, USA.
Chen
Chuan-Mu
CM
Shi
Huidong
H
Rahmatpanah
Farahnaz
F
Wei
Susan H
SH
Huang
Tim Hui-Ming
TH
D018899:CpG Islands; D019175:DNA Methylation; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis