title: [Experimental model for the evaluation of topical application of a solution to help myocutaneous flap transposition].
abstract: The aim of this article is the evaluation of the topical application of a solution of hydrogen peroxide (H2O2) 8% and dimethyl sulphoxide (DMSO) 50% in order to reduce ischaemic failure in random skin flaps. This study was performed using a rabbit model. Two parallel, cephalad-based para-midline random cutaneous flap (10 cm x 2.5 cm) were elevated and resutured in place on the dorsum of 40 New Zealand rabbits. The 80 flaps thus obtained were then randomly divided into one control group and three experimental groups of 20 flaps each. Flaps from the control group (group A) were topically treated with saline, while flaps from experimental group B were treated with H2O2 8%, flaps from experimental group C with DMSO 50%, and flaps from experimental group D with a solution of 50% DMSO + 8% H2O2. Each solution was topically applied, 20 cc per three times a day, on the flaps for seven days, starting on the immediate postoperative period. Transcutaneous oxygen tension (Ptc O2) measurements were carried out in all flaps, 72 h after flap elevation. The percentage of surviving skin area of each flap was determined by planimetry 7 days after flap elevation. The mean surviving area of the group A (control) flaps was 71%. The mean surviving area of the group B (H2O2-treated) flaps was 72%. The mean surviving area of the group C (DMSO-treated) flaps was 76%, and that of the group D (DMSO + H2O2-treated) flaps was 92%. While no statistically significant differences were found between the survival rates of both the flaps treated with H2O2 or DMSO alone and that of the control group, the mean surviving rate of the DMSO + H2O2 treated flaps (+20%) was statistically higher than that of the control flaps. Similarly, a statistically significant difference has been found between the mean Ptc O2 values of the DMSO + H2O2 flaps and those of the other three groups of flaps.
authors.affiliation: Chaire de chirurgie plastique et reconstructrice, institut national de recherches sur le cancer, Université de Gênes, 16153 Gênes, Italie.
authors.forename: E
authors.initials: E
authors.lastname: Raposio
authors.forename: P
authors.initials: P
authors.lastname: Caregnato
authors.forename: M
authors.initials: M
authors.lastname: Renzi
authors.forename: A
authors.initials: A
authors.lastname: Margara
authors.forename: G
authors.initials: G
authors.lastname: Ferrando
authors.forename: P
authors.initials: P
authors.lastname: Barabino
authors.forename: C
authors.initials: C
authors.lastname: Capello
authors.forename: A
authors.initials: A
authors.lastname: Gualdi
authors.forename: R
authors.initials: R
authors.lastname: Molinari
authors.forename: P L
authors.initials: PL
authors.lastname: Santi
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000627:therapeutic use*; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage / Q000627:therapeutic use*; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000627:therapeutic use*; D006085:Graft Survival; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000627:therapeutic use*; D007511:Ischemia / Q000517:prevention & control*; D011817:Rabbits; D011897:Random Allocation; D016038:Skin Transplantation; D013524:Surgical Flaps / Q000098:blood supply*
chemical_list: D000891:Anti-Infective Agents, Local; D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide; D004121:Dimethyl Sulfoxide