Consequence of aflatoxin B1 ingestion on the membrane-bound liver endoplasmic reticulum Ca2+-ATPase of protein-undernourished Fischer F344 rats.
Ingestion of the mycotoxin Aflatoxin B1 (AFB1) by protein-undernourished Fischer F344 rats for twelve weeks resulted in significant (p < 0.05) increases in the proliferation of liver cells, reduced body weight and increased microsomal Ca2+-ATPase activity. Cyt. P450 content, gamma-Glutamyl Transferase (GGT) and Glutathione Transferase (GST) activities were unaffected. The ingestion of AFB, by normal rats had no effect on all the parameters investigated. It appears that the microsomal Ca2+-pumping ATPase of Protein-Undernourished (PU) Fischer F344 rats is more sensitive to the mycotoxin AFB1 than its counterpart in well-nourished rats. The use of PU rat as animal model for studies on AFB, toxicity, particularly the effect of AFB1 on the regulation of intracellular calcium ion concentration ([Ca2+]), is suggested.
Adenuga
G A
GA
Department of Biochemistry, Olabisi Onabanjo University, Ago-lwoye, Ogun State, Nigeria.
D016604:Aflatoxin B1 / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D001835:Body Weight; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology*; D011502:Protein-Energy Malnutrition; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D003577:Cytochrome P-450 Enzyme System; D016604:Aflatoxin B1; D005723:gamma-Glutamyltransferase; D000252:Calcium-Transporting ATPases