Cholinergic innervation of parvalbumin- and calbindin-containing neurones in the hippocampus during postnatal development of the rat brain.
Immunohistochemical study of the cholinergic innervation of the parvalbumin- and calbindin-containing cells in the hippocampus was conducted on 30 rat brains of various postnatal ages: P0, P4, P7, P14, P21, P30, P60 and P180. Sections with double immunostaining for vesicular acetylcholine transporter (VAChT; the marker of cholinergic cells, fibres and terminals) and parvalbumin (PV) or calbindin (CB) were analysed using confocal laser-scanning microscope. Obtained data demonstrate that the pattern of cholinergic innervation of calbindin- and parvalbumin-immunoreactive hippocampal neurones shows some differences. During development as well as in the adult species cholinergic terminals preferentially innervate CB-containing neurones, while cholinergic terminals on PV-containing cells were observed rarely. Cholinergic endings on the CB-ir neurones are localised both on their somata and dendrites, whereas on PV-ir cells they form synaptic contact predominantly with processes. In spite of the unquestionable cholinergic influence particularly on CB-ir cells, the number of cholinergic endings suggests that this input seems not to be crucial for the activity of the studied cell populations.
Ludkiewicz
Beata
B
Department of Anatomy and Neurobiology, Medical University of Gdańsk, ul. Debinki 1, 80-211 Gdańsk, Poland.
Wójcik
Sławomir
S
Spodnik
Edyta
E
Domaradzka-Pytel
Beata
B
Klejbor
Ilona
I
Moryś
Janusz
J
D000818:Animals; D000831:Animals, Newborn; D064026:Calbindins; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002799:Cholinergic Fibers / Q000378:metabolism* / Q000648:ultrastructure; D020712:Fornix, Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D007395:Interneurons / Q000166:cytology / Q000378:metabolism*; D026901:Membrane Transport Proteins; D009433:Neural Inhibition / Q000502:physiology; D010320:Parvalbumins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D012686:Septal Nuclei / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D064026:Calbindins; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; C493461:Slc18a3 protein, rat; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D005680:gamma-Aminobutyric Acid