Aggregation of the Intracellular Domain of the Human Thrombopoietin Receptor c-MPL.
Thrombopoietin (TPO) is the major cytokine which is involved in platelet production and exerts its effects via the receptor c-MPL. The yeast two-hybrid system has been used to study the aggregation of the intracellular domain of c-MPL in TPO signal transduction. First, the cDNA fragment of MPLP intracellular domain was amplified and cloned by using RT-PCR method from the total RNA of HEL cells. Then the cDNA fragment was sequenced and subcloned into two-hybrid vectors pAS2 and pGAD424, respectively, and the recombinants are named as pASMM and pGADMM. Co-transformation of these plasmids into yeast activated his3 and lacZ reporter genes, demonstrating in vivo interaction of the MPLP receptor intracellular domain itself. The aggregation may be important in TPO signal transduction.
Zhao
Xin-Yan
XY
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China. xyzhao@fudan.edu.cn
Wang
Guang-Qing
GQ
Zhou
Wei-Guo
WG
Chen
Chun-Mei
CM
Li
Chang-Ben
CB
Zhao
Shou-Yuan
SY