title: Regulation of MTP expression in developing swine.
abstract: To define the developmental expression of microsomal triglyceride transfer protein (MTP) large subunit mRNA and protein, samples of small intestine and liver were collected from 40-day gestation fetal, 2-day-old newborn, 3-week-old suckling, and 2-month-old weanling swine. In fetal animals, MTP mRNA expression was high in intestine and liver. Postnatally, jejunal expression paralleled the intake of a high-fat breast milk diet and declined after weaning. Ileal expression was comparable with that of jejunum in 2-day-old animals, but declined to low levels afterward. Hepatic expression declined postnatally and remained low. MTP protein expression generally paralleled mRNA expression, except in fetal intestine in which no 97 kDa protein was detected. In 2-day-old piglets, a high-triacylglycerol diet increased jejunal and ileal MTP mRNA levels, as compared to a low-triacylglycerol diet. To test the roles of glucocorticoids and fatty acids in MTP regulation, a newborn swine enterocyte cell line (IPEC-1) was used. Except at day 2 of differentiation, dexamethasone did not influence MTP expression. Fatty acids either up-regulated or down-regulated MTP expression, depending on the specific fatty acid and duration of exposure. Although programmed genetic cues regulate MTP expression during development, clearly the amount and fatty acid composition of dietary lipid also play regulatory roles.
authors.affiliation: Children's Foundation Research Center of Memphis, Le Bonheur Children's Medical Center, Memphis, TN 38103, USA.
authors.forename: Song
authors.initials: S
authors.lastname: Lu
authors.forename: Mark
authors.initials: M
authors.lastname: Huffman
authors.forename: Ying
authors.initials: Y
authors.lastname: Yao
authors.forename: Charles M
authors.initials: CM
authors.lastname: Mansbach
authors.forename: Xiangying
authors.initials: X
authors.lastname: Cheng
authors.forename: Songmai
authors.initials: S
authors.lastname: Meng
authors.forename: Dennis D
authors.initials: DD
authors.lastname: Black
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; C106092:microsomal triglyceride transfer protein