Cloning and Sequencing of the Gene Encoding NS Protein of Wheat Rosette Stunt Virus.
The cDNA fragment located downstream of the WRSV N protein gene was obtained from the cDNA library of WRSV by hybridization with a 24 nucleotides sequence fragment of the 3' end of mRNA of the N protein. The cDNA fragment was sequenced and on ORF encoding a 40 kD protein was found. The gene was expressed in E. coli DE3 and was identified by Western blot experiment as the NS gene of WRSV.
Shen
Jia-Li
JL
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. gongzx@sunm.shcnc.ac.cn
Ye
Yong-Jun
YJ
Gong
Zu-Xun
ZX