title: A sensitive method for determining levels of [-]-2,5,-dimethoxy-4-methylamphetamine in the brain tissue.
abstract: BACKGROUND
  Indolamine and phenethylamine hallucinogens are drugs of abuse and, as well, mimic some aspects of idiopathic psychosis. To assist in investigating the mechanisms of action of (-)2,5-dimethoxy4-methylamphetamine ([-]-DOM), a member of the phenethylamine class of serotonergic hallucinogens, a sensitive and precise method for determining its levels in the brain tissue is required.
  
  
  METHODS
  We now describe a method for determining nanogram quantities of [-]-DOM in the rat brain tissue using D-amphetamine as an intemal standard. The method employs solvent extraction with toluene and derivatization with trifluoroacetic acid anhydride (TFAA) followed by analysis using gas chromatography-mass spectrometry (GS-MS) in the selective ion monitoring (SIM) mode.
  
  
  RESULTS
  With SIM detection, our overall recoveries were greater than 90%. The method was reliable in terms of within-day and between-day precision, accuracy, and linearity. The procedure was applied to animal subjects to determine the in vivo [-]-DOM brain levels following intraperitoneal (ip) administration. Our findings indicate that peak levels of [-]-DOM do not coincide with the 75-min pretreatment time established by drug-induced stimulus control.
  
  
  CONCLUSIONS
  This study demonstrates a sensitive and precise analytical method for the determination of [-]-DOM levels in the rat brain following systemic administration of behaviorally relevant doses.
authors.affiliation: Department of Pharmacology and Toxicology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, 14214-3000, USA. eckler@buffalo.edu
authors.forename: J R
authors.initials: JR
authors.lastname: Eckler
authors.forename: H
authors.initials: H
authors.lastname: Greizerstein
authors.forename: R A
authors.initials: RA
authors.lastname: Rabin
authors.forename: J C
authors.initials: JC
authors.lastname: Winter
mesh_terms: D004290:DOM 2,5-Dimethoxy-4-Methylamphetamine / Q000493:pharmacokinetics*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D003913:Dextroamphetamine / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D008401:Gas Chromatography-Mass Spectrometry; D006213:Hallucinogens / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D015203:Reproducibility of Results
chemical_list: D006213:Hallucinogens; D004290:DOM 2,5-Dimethoxy-4-Methylamphetamine; D003913:Dextroamphetamine