Nucleolar asynchrony observed in HL-60 leukemic granulocytic precursors resistant to 5-aminolaevulinic acid-based photodynamic treatment.
To provide more information on the 5-aminolaevulinic acid (ALA)-induced photodynamic effect on nucleoli, morphologically expressed nucleolar asynchrony (the presence of 'active' large nucleoli with an uniform distribution of RNA and 'resting' ring-shaped nucleoli in one and the same nucleus) was studied in cultured HL-60 leukemic granulocytic precursors using a simple cytochemical procedure for the demonstration of RNA. Nucleolar asynchrony was mainly expressed in cells which were apparently resistant to ALA-based photodynamic treatment (PDT) since most of them (about 75%) exhibited this phenomenon.
Smetana
Karel
K
Institute of Hematology and Blood Transfusion, 128 20 U nemocnice, Prague 2, Czech Republic. karel.smetana@uhkt.cz
Cajthamlová
Hana
H
Grebenová
Dana
D
Jirásková
Ilona
I
Hrkal
Zbynek
Z
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D002466:Cell Nucleolus / Q000187:drug effects* / Q000648:ultrastructure; D018922:HL-60 Cells; D006801:Humans; D010778:Photochemotherapy
D000622:Aminolevulinic Acid