Simple method for plant virus detection: effect of antibody immobilization technique.
The possibility has been demonstrated for applying a surface plasmon resonance for detecting plant viruses in real samples. An optimal mode for antiviral immunoglobulin immobilization on sensor surfaces is described. Out of three proposed techniques for sensor surface treatment, namely, unmodified gold surface, gold surface treated with (a) thiocyanate and (b) thiocyanate and protein A (Staphylococcus aureus), the latter was chosen as most suited for retention of the formed native immunoglobulin layer.
Boltovets
P M
PM
D.K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences, 145 Zabolotny St, Kyiv 03143, Ukraine. pboltovec@mail.ru
Boyko
V R
VR
Kostikov
I Yu
IY
Dyachenko
N S
NS
Snopok
B A
BA
Shirshov
Yu M
YM
D000914:Antibodies, Viral / Q000378:metabolism*; D000460:Chlorophyta / Q000821:virology*; D006046:Gold / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology*; D013205:Staphylococcal Protein A / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties; D013861:Thiocyanates / Q000378:metabolism; D014027:Tobacco Mosaic Virus / Q000302:isolation & purification* / Q000378:metabolism
D000914:Antibodies, Viral; D013205:Staphylococcal Protein A; D013861:Thiocyanates; D006046:Gold; C031760:thiocyanate