[The mechanism and application of posttranscriptional gene silencing].
The purpose of this review is to confirm the reason resulted the gene silence and explore the countermeasure avoiding the gene silence in transgene plant. The method is to divide the gene silencing into transcriptional gene silencing(TGS) and posttranscriptional gene silencing(PTGS). Several models resulted PTGS were analyzed by RNA threshold model, ectopic pairing and aberrant RNA model and ds-RNA model. The results showed that it was important to decide the phenomena of restraining transgene silencing and the mechanism of PTGS. The strategies of identification of gene function and prevention of virus were presented by RNAi and gene silencing respectively, etc.
Liu
Yang
Y
National Laboratory for Grassland Biotechnology, College of Life Science, Sichuan University, Chengdu 610064, China.
Jiang
Yan
Y
Qiao
Dai-Rong
DR
Cao
Yi
Y
D000818:Animals; D020868:Gene Silencing; D008957:Models, Genetic; D012313:RNA; D012323:RNA Processing, Post-Transcriptional
D012313:RNA