title: Detection of EB Virus Gene Fragments in Paraffin-embedded NPC Tissue.
abstract: To detect more sensibly Epstein-Barr virus (EBV) DNase gene fragments in nasopharyngeal carcinoma (NPC) tissue, a pair of optimized primers flanking 29% of EBV-DNase gene were designed. PCR results showed that EBV-DNase gene was detected in 145 of 149(97.32%) paraffin-embedded tissue samples with NPC and 2 of 155 (1.29%) cases in high risk population. DNA sequencing proved that DNA sequence of the PCR product agreed with BGLF5 reading frame from Genbank VO1555. Paraffin-embedded NPC samples stored for less than 1.5 years under room temperature and atmosphere conditions in South China were suitable for PCR detection.
authors.affiliation: Biochemistry Department, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China. cisums@gzsums.edu.cn
authors.forename: Di
authors.initials: D
authors.lastname: Huang
authors.forename: Bi-Jun
authors.initials: BJ
authors.lastname: Huang
authors.forename: Qiu-Liang
authors.initials: QL
authors.lastname: Wu
authors.forename: Shang-Wu
authors.initials: SW
authors.lastname: Chen
authors.forename: Jian-Quan
authors.initials: JQ
authors.lastname: Ma