The gene encoding the PSST subunit of respiratory chain complex I is present in more than one copy in yellow lupine.
Three copies of the PSST gene were identified in the lupine genomic root library, however, only two transcripts were found in the lupine root cDNA library. The transcript of the third PSST gene was identified in RNA from lupine flowers. The genes are 92% identical in the coding region, while the 5' parts of the reading frames specifying the N-terminal presequences for mitochondrial import show about 87% sequence identity. The differences between genes concern mostly the third nucleotide of the codons and the length of the intron. Transcripts of three PSST genes differ in abundance in flowers and leaves.
Piekna
Dorota
D
Institute of Molecular Biology and Biotechnology, A. Mickiewicz University, Miedzychodzka 5, 60-371 Poznań, Poland.
Sikorski
Michał
M
Augustyniak
Halina
H
D000595:Amino Acid Sequence; D004579:Electron Transport; D042967:Electron Transport Complex I; D007887:Fabaceae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D018628:Gene Dosage; D017343:Genes, Plant; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry; D021122:Protein Subunits; D018749:RNA, Plant / Q000096:biosynthesis; D016415:Sequence Alignment
D046911:Macromolecular Substances; D021122:Protein Subunits; D018749:RNA, Plant; D009247:NADH, NADPH Oxidoreductases; D009245:NADH Dehydrogenase; D042967:Electron Transport Complex I