title: Identification of two new alleles in a single Korean individual, HLA-B*1568 and HLA-DRB1*1208.
abstract: We have identified a new HLA-B*15 allele and a new HLA-DRB1*12 allele, named B*1568 and DRB1*1208, respectively. The alleles were identified using a combination of sequence specific primers, reverse line sequence specific oligonucleotide probing and sequence-based typing. Both alleles were identified in a single individual of Korean origin. HLA-B*1568 appears to be an HLA-B*4801/B*1507 hybrid combining the exon 2 sequence of B*4801 and the exon 3 and 4 sequences of B*1507. Exon 2 of DRB1*1208 was most similar to DRB1*1201 or 1206, with a single mismatch at nucleotide position 165 (A to C). At the protein level, this substitution results in a phenylalanine substitution at position 26 that creates an identical amino acid sequence to DRB3*0202 between amino acid positions 17 and 36.
authors.affiliation: Dynal Biotech Ltd, Wirral, UK. melanie.sheldon@dynalbiotech.com
authors.forename: M H
authors.initials: MH
authors.lastname: Sheldon
authors.forename: M
authors.initials: M
authors.lastname: Bunce
authors.forename: P P J
authors.initials: PP
authors.lastname: Dunn
authors.forename: S
authors.initials: S
authors.lastname: Day
authors.forename: G D
authors.initials: GD
authors.lastname: Lee
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Park
authors.forename: B K
authors.initials: BK
authors.lastname: Bang
authors.forename: B K
authors.initials: BK
authors.lastname: Kim
authors.forename: E-J
authors.initials: EJ
authors.lastname: Oh
mesh_terms: D000595:Amino Acid Sequence; D005091:Exons; D015235:HLA-B Antigens / Q000235:genetics*; D059910:HLA-B15 Antigen; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D007723:Korea; D008969:Molecular Sequence Data
chemical_list: D015235:HLA-B Antigens; C471429:HLA-B*15:68 antigen; D059910:HLA-B15 Antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C471430:HLA-DRB1*12:08 antigen