Retardation of the G2-M phase progression on gene disruption of RNA binding protein Sam68 in the DT40 cell line.
Sam68 is an RNA binding protein that is tyrosine-phosphorylated by Src during mitosis and has been postulated to have a role in cell cycle control by modulating RNA metabolism. To elucidate the function of this protein, we isolated a Sam68-deficient DT40 cell line by gene disruption. The Sam68-deficient cells exhibited markedly decreased growth and the growth retardation was due to elongation of the G2-M phase, however, the kinase activity associated with Cdc2 remained unaltered. Our results indicate that Sam68 may play a critical role in G2-M progression in a manner independent of the control of Cyclin/Cdc2 kinase activity.
Li
Qing-Hua
QH
Department of Microbiology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, 570-8506, Osaka, Japan.
Haga
Izumi
I
Shimizu
Toshiki
T
Itoh
Michiyasu
M
Kurosaki
Tomohiro
T
Fujisawa
Jun-ichi
J
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D002645:Chickens; D002999:Clone Cells / Q000166:cytology / Q000378:metabolism; D004789:Enzyme Activation / Q000502:physiology; D016195:G2 Phase / Q000502:physiology*; D018390:Gene Targeting; D008938:Mitosis / Q000502:physiology*; D008969:Molecular Sequence Data; D016601:RNA-Binding Proteins / Q000235:genetics*
D016601:RNA-Binding Proteins; D016203:CDC2 Protein Kinase