Isolation and Cloning of 17 Novel C(2)H(2) Zinc Finger Gene Fragments.
A pair of degenerate primers were designed according to the DNA sequences of the C(2)H(2) zinc finger genes conservative domain and then homologious PCR was performed with the human genomic total DNA as template. The zinc finger fragments thus obtained were used as probes to screen cDNA libraries of human fetal kidney, muscle and marrow and 22 C(2)H(2) zinc finger gene fragments were selected. Among these fragments, 17 were confirmed to be novel zinc finger gene fragments by literature searches in the National Center Biotechnology Information (NCBI) database. Expression patterns of the clones K3-4 and K5-12 selected from kidney cDNA library were analyzed. The results showed that the expression level of K3-4 in kidney is obviously higher than in other tissues and K5-12 is expressed at different levels in 8 tissues.
Zhang
Min
M
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China. longyu@ms.fudan.edu.cn
Yu
Long
L
Hu
Pei-Rong
PR
Wang
Xiao-Ke
XK
Bi
An-Ding
AD
Chen
Qi
Q
Zhao
Shou-Yuan
SY