Role of trehalose biosynthesis in environmental survival and virulence of Salmonella enterica serovar typhimurium.
The otsA and otsB genes, encoding trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase respectively, have been isolated from Salmonella enterica serovar typhimurium and nucleotide-sequenced. Induction of trehalose biosynthesis by exposure of bacteria to high osmotic strength resulted in the intracellular accumulation of trehalose. An otsA mutant of S. enterica serovar typhimurium was more susceptible to killing by heat, and grew poorly under conditions of high osmolarity. The wild-type and otsA mutant strains showed similar abilities to colonise spleen tissues after oral dosing of mice. These findings suggest that the otsBA gene products play a role in environmental survival, but not in virulence, of S. enterica serovar typhimurium.
Howells
Angela M
AM
Defence Science and Technology Laboratory, CBS Porton Down, Salisbury, Wilts, UK.
Bullifent
Helen L
HL
Dhaliwal
Kam
K
Griffin
Kate
K
García de Castro
Arcadio
A
Frith
Graeme
G
Tunnacliffe
Alan
A
Titball
Richard W
RW
D000818:Animals; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D005260:Female; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D051379:Mice; D008807:Mice, Inbred BALB C; D016254:Mutagenesis, Insertional; D009876:Operon / Q000235:genetics; D009997:Osmotic Pressure; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D014199:Trehalose / Q000096:biosynthesis* / Q000378:metabolism; D014774:Virulence
D004269:DNA, Bacterial; D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase; C023178:trehalose-phosphatase; D010744:Phosphoric Monoester Hydrolases