title: Complement receptor 3 (CD11b/CD18) mediates type I and type II phagocytosis during nonopsonic and opsonic phagocytosis, respectively.
abstract: Two types of opsonic phagocytosis have been defined depending on the receptor engaged: FcgammaRs mediate type I phagocytosis of IgG-coated particles; complement receptor 3 (CR3) mediates type II phagocytosis of complement-coated particles. In addition to opsonic phagocytosis, CR3 also mediates nonopsonic phagocytosis of zymosan (Z) and Mycobacterium kansasii through engagement of distinct sites. Using Chinese hamster ovary cells stably expressing human CR3, we studied CR3-mediated ingestion of nonopsonized particles, Z or M. kansasii, compared with opsonized zymosan (OZ). We show that 1) while OZ sinks into cells, Z is engulfed by pseudopodia as visualized by electron microscopy; 2) in contrast to OZ, nonopsonic phagocytosis of Z and M. kansasii depends on Rac and Cdc42 but not on Rho activity; and 3) CR3-mediated phagocytosis of Z depends on the kinase activity of the Src family tyrosine kinase Hck, while OZ internalization does not. Therefore, CR3 mediates type I phagocytosis under nonopsonic conditions and type II under opsonic conditions. This is the first evidence that a single receptor can mediate both types of phagocytosis depending on the ligand used.
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, Centre National de la Recherche Scientifique, Toulouse, France. Veronique.Le.Cabec@ipbs.fr
authors.forename: Véronique
authors.initials: V
authors.lastname: Le Cabec
authors.forename: Sébastien
authors.initials: S
authors.lastname: Carréno
authors.forename: André
authors.initials: A
authors.lastname: Moisand
authors.forename: Christine
authors.initials: C
authors.lastname: Bordier
authors.forename: Isabelle
authors.initials: I
authors.lastname: Maridonneau-Parini
mesh_terms: D000818:Animals; D016466:CHO Cells; D003165:Complement System Proteins / Q000378:metabolism; D006224:Cricetinae; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000378:metabolism*; D008854:Microscopy, Electron; D019909:Mycobacterium kansasii / Q000276:immunology; D009895:Opsonin Proteins / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D015398:Signal Transduction; D014162:Transfection; D015054:Zymosan / Q000276:immunology; D019061:src-Family Kinases / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D016177:Macrophage-1 Antigen; D009895:Opsonin Proteins; D011994:Recombinant Proteins; D003165:Complement System Proteins; D015054:Zymosan; D019061:src-Family Kinases