Identification of two new alleles in a single Korean individual, HLA-B*1568 and HLA-DRB1*1208.
We have identified a new HLA-B*15 allele and a new HLA-DRB1*12 allele, named B*1568 and DRB1*1208, respectively. The alleles were identified using a combination of sequence specific primers, reverse line sequence specific oligonucleotide probing and sequence-based typing. Both alleles were identified in a single individual of Korean origin. HLA-B*1568 appears to be an HLA-B*4801/B*1507 hybrid combining the exon 2 sequence of B*4801 and the exon 3 and 4 sequences of B*1507. Exon 2 of DRB1*1208 was most similar to DRB1*1201 or 1206, with a single mismatch at nucleotide position 165 (A to C). At the protein level, this substitution results in a phenylalanine substitution at position 26 that creates an identical amino acid sequence to DRB3*0202 between amino acid positions 17 and 36.
Sheldon
M H
MH
Dynal Biotech Ltd, Wirral, UK. melanie.sheldon@dynalbiotech.com
Bunce
M
M
Dunn
P P J
PP
Day
S
S
Lee
G D
GD
Park
Y-J
YJ
Bang
B K
BK
Kim
B K
BK
Oh
E-J
EJ
D000595:Amino Acid Sequence; D005091:Exons; D015235:HLA-B Antigens / Q000235:genetics*; D059910:HLA-B15 Antigen; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D007723:Korea; D008969:Molecular Sequence Data
D015235:HLA-B Antigens; C471429:HLA-B*15:68 antigen; D059910:HLA-B15 Antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C471430:HLA-DRB1*12:08 antigen