title: Characterization of neutralization specificities of outer capsid spike protein VP4 of selected murine, lapine, and human rotavirus strains.
abstract: Neutralization specificities of outer capsid spike protein VP4 of murine rotavirus strains EW (P?[16],G3) and EHP (P?[20],G3) and lapine rotavirus strains Ala (P?[14],G3), C11 (P?[14],G3), and R2 (P?[14],G3) as well as human rotavirus strains PA169 (P?[14],G6) and HAL1166 (P?[14],G8) were determined by two-way cross-neutralization. This was done by generating and characterizing (i) three murine x human, three lapine x human, and two human x human single gene substitution reassortant rotaviruses, each of which bore identical human rotavirus DS-1 strain VP7 (G2), and (ii) guinea pig hyperimmune antiserum raised against each reassortant. Reference rotavirus strains employed in the study represented 10 established VP4 (P) serotypes, including 1A[8], 1B[4], 2A[6], 3[9], 4[10], 5A[2], 5B[2], 5B[3], 6[1], 7[5], 8[11], 9[7], and 10[16] as well as a P serotype unknown P[18]. Murine rotavirus strains EW and EB were demonstrated to share the same P serotype (P10[16]) distinct from (i) 9 established P serotypes, (ii) lapine and human rotavirus strains bearing the P[14] genotype, and (iii) an equine rotavirus strain bearing the P[18] genotype. Both lapine (Ala, C11, and R2) and human (PA169 and HAL1166) rotaviruses were shown to belong to the same VP4 serotype, which represented a distinct new P serotype (P11[14]). P serotype 13[20] was assigned to murine rotavirus EHP strain VP4, which was shown to be distinct from all the P serotypes/genotypes examined in the present study.
authors.affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-8026, USA. THOSHINO@niaid.nih.gov
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Jones
authors.forename: Albert Z
authors.initials: AZ
authors.lastname: Kapikian
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D005838:Genotype; D006801:Humans; D008253:Macaca mulatta; D051379:Mice; D009500:Neutralization Tests; D011817:Rabbits; D016865:Reassortant Viruses / Q000145:classification* / Q000235:genetics / Q000276:immunology; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D012703:Serotyping
chemical_list: D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus