[Study of alginate/chitosan microcapsules for immobilization of Escherichia coli DH5 alpha].
Objective proteins synthesized from genetically recombined Escherichia coli strain (E. coli) have been successfully produced by microbe fermentation, but complicated separation and purification steps always make against the maintenance of activities as well as increase the cost. Aiming at simplifying the process, an idea of administrating directly the microencapsulated genetically recombined E. coli is proposed. In this paper, study on culture of E. coli DH5 alpha immobilized in alginate/chitosan (ACA) microcapsule is presented. It was found that E. coli DH5 alpha grew well in the microcapsule with stable growth period longer than that of suspension culture, and cell aggregation phenomenon was observed. In vivo experiments showed that ACA microcapsules with E. coli DH5 alpha stayed over 48 h in mouse intestine, and the morphology of microcapsules was kept intact. These preliminary results have demonstrated that administration of microencapsulated E. coli DH5 alpha is safe, which laied the foundation for microencapsulated genetically recombined E. coli as carriers of gene engineering drugs.
Fu
Ying-Li
YL
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Xiong
Ying
Y
Liu
Xiu-Dong
XD
Yu
Wei-Ting
WT
Wang
Yi-Li
YL
Yu
Xing-Ju
XJ
Ma
Xiao-Jun
XJ
D000464:Alginates; D000818:Animals; D002214:Capsules; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D016503:Drug Delivery Systems; D004926:Escherichia coli / Q000254:growth & development / Q000502:physiology; D018014:Gene Transfer Techniques; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D051379:Mice
D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D002686:Chitin; D020723:Glucuronic Acid; D048271:Chitosan