Increase of immunoglobulin productivity of human-human hybridoma HB4C5 cells by histone.
A histone mixture (H1, H2A, H2B, H3, and H4) derived from calf thymus stimulated IgM production by human-human hybridoma HB4C5 cells. On the contrary, the histone mixture did not increase IgM production by the human Burkitt's lymphoma cell line NAT-30, IgG production by the human B lymphoblastoid cell line HMy-2, and IgE production by the human myeloma cell line U266. The immunoglobulin production-stimulating activity of the histone mixture was inactivated by trypsin or chymotrypsin digestion. In addition, confocal laser microscopic analysis had shown that HB4C5 cells incorporated a lot of histone but other cell lines did not incorporate it as much. These facts strongly suggest that histone acts as an immunoglobulin production-stimulating factor (IPSF) after internalization into the human B cell lines and the native structure of histone is required for the IPSF activity.
Okamoto
Takeaki
T
Department of Bioscience and Biotechnology, Graduate School of Kyushu University, Hakozaki, Fukuoka, Japan.
Sugahara
Takuya
T
Tachibana
Hirofumi
H
Yamada
Koji
K
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D002051:Burkitt Lymphoma / Q000276:immunology / Q000378:metabolism; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006657:Histones / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D006825:Hybridomas / Q000187:drug effects* / Q000378:metabolism*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D009101:Multiple Myeloma / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D021381:Protein Transport; D013950:Thymus Gland; D014357:Trypsin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D006657:Histones; D007075:Immunoglobulin M; D002918:Chymotrypsin; D014357:Trypsin