Second-harmonic generation from biological tissues: effect of excitation wavelength.
We report on the measurement of second-harmonic signals from hyperplastic parenchyma and stroma in malignant human prostate tissue under femtosecond pulsed illumination in the wavelength range from 730 to 870 nm. In particular, the relationship of the second-harmonic generation to the excitation wavelength is measured. The result in these two regions behaves considerably differently and thus provides a possible indicator for identifying tissue components and malignancy.
Deng
Xiaoyuan
X
Centre for Micro-Photonics, School of Biophysical Sciences and Electrical Engineering, Swinburne University of Technology, Hawthorn, Victoria, Australia.
Williams
Elizabeth D
ED
Thompson
Erik W
EW
Gan
X
X
Gu
Min
M
D006801:Humans; D007834:Lasers; D008297:Male; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D011467:Prostate / Q000648:ultrastructure*; D011470:Prostatic Hyperplasia / Q000175:diagnosis / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000473:pathology*; D013055:Spectrophotometry, Infrared / Q000295:instrumentation / Q000379:methods