title: Antioxidative activity of spin labeled derivatives of podophyllic acid hydrazide.
abstract: OBJECTIVE
  To study the relationship between structure and antioxidation activity of spin labeled derivatives of podophyllic acid hydrazide (GP) in tissues and red blood cells (RBC) from rats.
  
  
  METHODS
  The homogenate of liver, heart, and kidneys of rats was used to measure malondialdehyde (MDA) spontaneous generated and induced by hydroxyl free radical generation system (Fe2+-ascorbic acid, FRGS) or doxorubicin (DOX) by TBA colorimetric method. H2O2-caused hemolysis was determined spectrophotometrically. Superoxide anion from zymosan-stimulated neutrophils of rats was evaluated by NBT-reduction assay.
  
  
  RESULTS
  GP1 and GP1OH obviously inhibited MDA formation either spontaneously or induced by FRGS and DOX and antagonized hemolysis induced by H2O2, but GP and GP1H showed less potent activity. GP1 also inhibited the formation of superoxide anion from activated neutrophils of rats.
  
  
  CONCLUSIONS
  Introduction of nitroxyl radical moieties into GP generated potent derivatives with antioxidative activity. The essential antioxidation active groups of spin labeled derivative of GP are NO or NOH group in nitroxyl radical moieties.
authors.affiliation: Department of Pharmacology, Department of Physiology, Lanzhou Medical College, Lanzhou 730000, China. wyj@lz.gs.cninfo.net
authors.forename: Wen-Guang
authors.initials: WG
authors.lastname: Li
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Zhang
authors.forename: Yong-Jie
authors.initials: YJ
authors.lastname: Wu
authors.forename: Xuan
authors.initials: X
authors.lastname: Tian
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D011033:Podophyllin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011034:Podophyllotoxin / Q000031:analogs & derivatives; D051381:Rats; D017208:Rats, Wistar; D013113:Spin Labels; D019097:Spin Trapping
chemical_list: D000975:Antioxidants; D013113:Spin Labels; D008315:Malondialdehyde; C003170:mitopodozide; D011033:Podophyllin; D011034:Podophyllotoxin