title: [The electron microscopic in situ hybridization and its application].
abstract: The technique of electron microsco-pic in situ hybridization is applying in situ hybridization at the electron microscopic level. It is mainly used in the ultrastructural localization of the lablled DNA, RNA and RHA in a cell and/or a tissue. In this paper I mainly elaborated its establishment and classification, and the operation procedure of nonradioactive electron microscopic in situ hybridization and some points for attention. In the end I also discussed its application for research.
authors.affiliation: Institution of Plant Gene and Gene Technology, School of Life Sciences, Xiamen University, Xiamen 361005, China. ctxie@163.net
authors.forename: Chao-Tian
authors.initials: CT
authors.lastname: Xie
authors.forename: Sheng-Chang
authors.initials: SC
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Miao
authors.forename: De-Hai
authors.initials: DH
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
mesh_terms: D017403:In Situ Hybridization / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*