Genotyping of methicillin-resistant Staphylococcus aureus by assaying for the presence of variable elements associated with mecA.
The region surrounding mecA in methicillin-resistant Staphylococcus aureus (MRSA) is highly variable. We describe an approach for the rapid genotyping of MRSA by assaying for the presence or absence of variable or mobile elements previously shown to be associated with the mecA region.
Huygens
Flavia
F
Cooperative Research Centre for Diagnostics, Queensland University of Technology, Brisbane, Queensland 4001, Australia.
Nimmo
Graeme R
GR
Schooneveldt
Jacqueline
J
Munckhof
Wendy J
WJ
Giffard
Philip M
PM
D001315:Australia / Q000453:epidemiology / Q000208:ethnology; D001426:Bacterial Proteins; D015373:Bacterial Typing Techniques; D002352:Carrier Proteins / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006602:Hexosyltransferases; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D016133:Polymerase Chain Reaction / Q000379:methods*; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000208:ethnology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase