Optimization strategies for HPLC enantioseparation of racemic drugs using polysaccharides and macrocyclic glycopeptide antibiotic chiral stationary phases.
The chiral resolution by high performance liquid chromatography (HPLC) is controlled by a number of parameters. The optimization of HPLC parameters is an important issue in chiral resolution. This review discusses the optimization of HPLC conditions for the chiral resolution of racemic drugs on polysaccharides and macrocyclic glycopeptide antibiotic chiral stationary phases (CSPs). The most important parameters discussed are composition of mobile phase, pH of mobile phase, flow rate, temperature and effect of other parameters.
Aboul-Enein
Hassan Y
HY
Biological and Medical Research Department (MBC-03), King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. enein@kfshrc.edu.sa
Ali
Imran
I
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006020:Glycopeptides; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D011134:Polysaccharides / Q000737:chemistry*; D013237:Stereoisomerism; D013696:Temperature
D000900:Anti-Bacterial Agents; D002021:Buffers; D006020:Glycopeptides; D004364:Pharmaceutical Preparations; D011134:Polysaccharides