The CRK1 receptor-like kinase gene of tobacco is negatively regulated by cytokinin.
This report describes a novel receptor-like kinase gene of tobacco (Nicotiana tabacum L.) that, in cell culture, is rapidly regulated by very low concentrations of cytokinin. The steady-state transcript level of the CYTOKONIN-REGULATED KINASE 1 gene (CRK1) was strongly reduced 30 min after cytokinin treatment. At higher concentrations abscisic acid and auxin induced a similar response. None of the other plant hormones tested elicited this response. Further analyses of the cytokinin-dependentregulation showed that the reduction of transcript was transient, and the duration of the recovery phase was dependent on the hormone concentration. CRKI is not a primary response gene as the simultaneous addition of cycloheximide inhibits its regulation by cytokinin. Inhibitor studies revealed that a protein phosphatase is likely involved in signalling processes upstream of CRK1. CRKI is expressed at low levels in the leaves, stem and roots of tobacco. It is predicted that the CRK1 protein is located in the plasma membrane. It has in its N-terminal putative receptor sequence a signal peptide, a serine- and a proline-rich region, a six repeat motif similar to the CRINKLY4 protein of Zea mays and several regions homologous to purine-binding motifs. A single transmembrane domain is followed by a highly conserved intracellular Ser/Thr kinase domain. Therefore, CRKI is a novel type of class I plant receptor kinase. We hypothesize that CRKI is involved in an early step of hormone signalling and that transcript down-regulation reflects a desensitization step in reaction to the signalling molecule.
Schäfer
Silke
S
Universität Tübingen, Centre for Plant Molecular Biology (ZMPB), Allgemeine Genetik, Germany.
Schmülling
Thomas
T
D001483:Base Sequence; D003001:Cloning, Molecular; D003583:Cytokinins / Q000494:pharmacology*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011494:Protein Kinases / Q000235:genetics*; D018749:RNA, Plant / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
D003583:Cytokinins; D018076:DNA, Complementary; D010937:Plant Growth Regulators; D018749:RNA, Plant; D011494:Protein Kinases