Induction and transmission of cytogenetic toxic effects of 5-fluorouracil in male germline cells of Swiss mice.
Cytogenetic toxicity after a single intraperitoneal exposure of three different doses (5,10 and 15 mg/kg) of 5-fluorouracil (5-FU) and its transmission in the male germline cells of Swiss mice was assessed. At 24 hrs post-treatment each of the doses of 5-FU induced statistically highly significant number of chromosomal aberrations, mostly random chromatid breaks, in the spermatogonial cells with maximum aberrations in the lowest dose. Primary spermatocytic chromosome analysis at week 4 post-treatment showed the presence of a statistically significant number of aberrant spermatocytes with atypical bivalents, mostly with autosomal and/or XY univalents. Sperm morphology assay at week 8 post-treatment exhibited higher percentages of abnormal sperm, but were not statistically significant. This indicated the gradual decline in the transmission of the induced cytogenetic toxic effects of 5-FU from spermatogonia to sperm, which might be because of gradual elimination of the grossly affected spermatogonial cells during the course of spermatogenesis.
Choudhury
R C
RC
Dept. of Zoology, Berhampur University, Orissa, India. rameshchoudary@hotmail.com
Misra
S
S
Jagdale
M B
MB
Palo
A K
AK
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000633:toxicity*; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity; D002869:Chromosome Aberrations / Q000139:chemically induced*; D003520:Cyclophosphamide / Q000633:toxicity; D005472:Fluorouracil / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D013090:Spermatocytes / Q000187:drug effects* / Q000473:pathology; D013093:Spermatogonia / Q000187:drug effects* / Q000473:pathology; D013094:Spermatozoa / Q000187:drug effects*
D000964:Antimetabolites, Antineoplastic; D018906:Antineoplastic Agents, Alkylating; D003520:Cyclophosphamide; D005472:Fluorouracil