title: Dioxin catabolic genes are dispersed on the Terrabacter sp. DBF63 genome.
abstract: Reverse transcription-PCR of the dbfA1A2, dbfBC, and pht genes, encoding oxygenase component of multicomponent dioxygenase, meta cleavage enzyme and hydrolase, and phthalate-degrading enzymes, respectively, revealed their role in the aromatic compound degradation by Terrabacter sp. strain DBF63. The specific expression in strain DBF63 cells grown on dibenzofuran (the model compound of dioxin; DF) and/or fluorene (FN) indicated that the DbfA1A2 and DbfBC catalyze the conversion of DF to salicylate, and that the DbfA1A2 and Pht enzymes are involved in FN degradation. Pulsed-field gel electrophoresis analyses revealed that the dbfA1A2 cistron and pht operon were located on the two linear plasmids, pDBF1 (160 kb) and pDBF2 (190 kb), while dbfBC genes were located on the chromosome. Because the pht operon is located immediately upstream of the dbfA1A2 cistron, the dioxin-catabolic genes were dispersed on the genome of strain DBF63, while FN-catabolic genes were gathered on the plasmids.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
authors.forename: Mayuko
authors.initials: M
authors.lastname: Kamakura
authors.forename: Masaaki
authors.initials: M
authors.lastname: Urata
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tanaka
authors.forename: Jin-Sung
authors.initials: JS
authors.lastname: Chung
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Takemura
authors.forename: Takako
authors.initials: T
authors.lastname: Yoshida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
authors.forename: Toshio
authors.initials: T
authors.lastname: Omori
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001572:Benzofurans / Q000378:metabolism; D001673:Biodegradation, Environmental; D002273:Carcinogens / Q000378:metabolism; D004147:Dioxins / Q000378:metabolism*; D005449:Fluorenes / Q000378:metabolism; D005798:Genes, Bacterial; D015394:Molecular Structure; D016366:Open Reading Frames; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D010795:Phthalic Acids / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D001572:Benzofurans; D002273:Carcinogens; D004147:Dioxins; D005449:Fluorenes; D010795:Phthalic Acids; C041509:fluorene; C032279:phthalic acid; C023614:dibenzofuran; D010105:Oxygenases