title: Purification and properties of arylsulphatase from the brain of the silkworm Bombyx mori.
abstract: (1) Arylsulphatase of the silkworm Bombyx mori was partially purified using ammonium sulphate fractionation, ethanol precipitation, Sephadex G-200 gel filtration and Con-A Sepharose chromatography. (2) The purified enzyme preparation was not homogeneous but showed no beta-glucuronidase or beta-galactosidase activities. (3) The kinetic properties of the enzyme indicated that it could be classified under type-2 arylsulphatases of vertebrates. (4) The purified enzyme shows very little activity towards p-nitrophenyl sulphate and none towards cerebroside 3-sulphate.
authors.affiliation: Department of Zoology, Aligarh Muslim University, Aligarh, 202001 India.
authors.forename: A A
authors.initials: AA
authors.lastname: Farooqui
authors.forename: A N
authors.initials: AN
authors.lastname: Yusufi
mesh_terms: D000818:Animals; D000838:Anions / Q000494:pharmacology; D001192:Arylsulfatases / Q000737:chemistry* / Q000302:isolation & purification; D012831:Bombyx / Q000201:enzymology*; D001921:Brain / Q000201:enzymology*; D001923:Brain Chemistry; D002396:Catechols / Q000737:chemistry; D002413:Cations, Divalent / Q000494:pharmacology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D004795:Enzyme Stability / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007814:Larva; D012965:Sodium Chloride / Q000494:pharmacology; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D000838:Anions; D002396:Catechols; D002413:Cations, Divalent; D012965:Sodium Chloride; D001192:Arylsulfatases; C021228:4-nitrocatechol sulfate