Increased thermosensitivity of mouse colorectal carcinoma cells transfected with human FUT1 gene.
The thermal responses of mouse colorectal carcinoma cells were investigated in the wild type cells and the transfected cells with human FUT1 gene which encodes alpha 1,2fucosyltransferase. The heat sensitivity was observed to increase in the FUT1 gene transfected cells and the effect of hyperthermia at 44 degrees C on these cells was demonstrated to be significant (P<0.001) to the wild type cells even though no remarkable difference in the expression of the heat shock protein, Hsp70 was found in these cells. Thus the expression of alpha 1,2fucosylated antigens seemed to increase the heat sensitivity in mouse colorectal carcinoma cells.
Okamura
Akihiko
A
First Department of Surgery, Gunma University Faculty of Medicine, 3-39-15 Showa-machi, Maebashi 371-8511, Japan. kaukau@tohgoku.or.jp
Yazawa
Shin
S
Morinaga
Nobuhiro
N
Asao
Takayuki
T
Kuwano
Hiroyuki
H
D000818:Animals; D015179:Colorectal Neoplasms / Q000473:pathology / Q000628:therapy*; D005434:Flow Cytometry; D005647:Fucosyltransferases / Q000235:genetics / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006801:Humans; D006979:Hyperthermia, Induced; D051379:Mice; D014162:Transfection; D000097631:Galactoside 2-alpha-L-fucosyltransferase
D005647:Fucosyltransferases; D018840:HSP70 Heat-Shock Proteins; D000097631:Galactoside 2-alpha-L-fucosyltransferase