title: Species-specific proteins identified in Ascaris lumbricoides and Ascaris suum using two-dimensional electrophoresis.
abstract: The protein profile of adult female Ascaris lumbricoides and Ascaris suum originating from humans and pigs, respectively, was studied using two-dimensional polyacrylamide gel electrophoresis. Six different major protein spots specific for A. lumbricoides were identified irrespective of their geographical origin and no major specific spot was encountered in A. suum. No major differences in the protein profiles between the extract by phosphate-buffered saline and urea were encountered for either Ascaris species. It is therefore possible to use 2D-PAGE as a tool for discriminating the closely related Ascaris species from humans and pigs.
authors.affiliation: Laboratory of Parasitic Diseases, National Institute of Animal Health, National Agricultural Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Wossene
authors.initials: W
authors.lastname: Abebe
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Tsuji
authors.forename: Harue
authors.initials: H
authors.lastname: Kasuga-Aoki
authors.forename: Takeharu
authors.initials: T
authors.lastname: Miyoshi
authors.forename: Takashi
authors.initials: T
authors.lastname: Isobe
authors.forename: Takeshi
authors.initials: T
authors.lastname: Arakawa
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Shinobu
authors.initials: S
authors.lastname: Yoshihara
mesh_terms: D000818:Animals; D017164:Ascaris lumbricoides / Q000302:isolation & purification / Q000502:physiology; D017165:Ascaris suum / Q000302:isolation & purification / Q000502:physiology; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D019143:Evolution, Molecular; D005260:Female; D015801:Helminth Proteins / Q000032:analysis*; D006801:Humans; D013045:Species Specificity; D013552:Swine
chemical_list: D015801:Helminth Proteins