title: Insect cell culture medium supplementation with fetal bovine serum and bovine serum albumin: effects on baculovirus adsorption and infection kinetics.
abstract: The effects of insect cell culture medium supplementation with FBS were investigated. BSA was found to be the factor responsible for the increased baculovirus infection rate of FBS-supplemented cultures in a concentration-dependent form up to 25 g L(-)(1). Lower rates of baculovirus binding to cells were observed with FBS- and BSA-supplemented cultures compared with infections carried out in serum-free media. Virus attachment constants were found to depend on medium matrix composition. An efficiency factor dependent on the medium matrix composition was introduced to account for these effects, and a mathematical model was developed to describe the virus-cell interactions. It was shown that BSA acts by minimizing the nonspecific virus binding leading to an increased cell infection rate. Cell specific Porcine parvovirusvirus-like particles (PPV-VLPs) expression was unaffected by medium supplementation pointing out that BSA and/or FBS affects mainly the initial phase of the baculovirus infection cycle. Implications for process definition are discussed.
authors.affiliation: Instituto de Biologia Experimental e Tecnológica/Instituto de Tecnologia Química e Biológica IBET/ITQB, Apartado 12, P-2781-901 Oeiras, Portugal.
authors.forename: Luis
authors.initials: L
authors.lastname: Maranga
authors.forename: Ana S
authors.initials: AS
authors.lastname: Coroadinha
authors.forename: Manuel J T
authors.initials: MJ
authors.lastname: Carrondo
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D016367:Baculoviridae / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D005312:Fetal Blood / Q000737:chemistry; D015870:Gene Expression; D007700:Kinetics; D008954:Models, Biological; D008967:Molecular Biology / Q000379:methods; D012710:Serum Albumin, Bovine / Q000302:isolation & purification / Q000494:pharmacology*; D018411:Spodoptera / Q000166:cytology / Q000187:drug effects* / Q000821:virology*
chemical_list: D003470:Culture Media; D012710:Serum Albumin, Bovine