title: Hypothermia inhibits cell proliferation and nitric oxide synthase expression in rats.
abstract: In the present study, the effects of cold-water immersion on cell proliferation and nitric oxide synthase expression in the dentate gyrus of rats were investigated. Sprague-Dawley rats were divided into four groups: the control-rest group; the control-heat group; the cold-rest group; and the cold-heat group. Cold-water immersion for 5 min at 4 degrees C suppressed the numbers of 5-bromo-2'-deoxyuridine-positive and nicotinamide adenine dinucleotide phosphate-diaphorase-positive cells in the dentate gyrus, and these numbers were increased by warming for 30 min at 30 degrees C. In the present study, it was demonstrated that warming protects against cold stress-induced suppression of new cell formation, and results suggest that nitric oxide, the synthesis of which is affected adversely by cold-water immersion, may play an important role in the regulation of cell proliferation.
authors.affiliation: Department of Physiology, College of Medicine, Kyung Hee University, 1 Hoigi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
authors.forename: Kwang-Sik
authors.initials: KS
authors.lastname: Lee
authors.forename: Baek-Vin
authors.initials: BV
authors.lastname: Lim
authors.forename: Mi-Hyeon
authors.initials: MH
authors.lastname: Jang
authors.forename: Min-Chul
authors.initials: MC
authors.lastname: Shin
authors.forename: Taeck-Hyun
authors.initials: TH
authors.lastname: Lee
authors.forename: Young-Pyo
authors.initials: YP
authors.lastname: Kim
authors.forename: Ho-Su
authors.initials: HS
authors.lastname: Shin
authors.forename: Seong-Yeon
authors.initials: SY
authors.lastname: Cho
authors.forename: Hong
authors.initials: H
authors.lastname: Kim
authors.forename: Mal-Soon
authors.initials: MS
authors.lastname: Shin
authors.forename: Ee-Hwa
authors.initials: EH
authors.lastname: Kim
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D000963:Antimetabolites / Q000032:analysis; D001973:Bromodeoxyuridine / Q000032:analysis; D002455:Cell Division / Q000502:physiology; D003080:Cold Temperature; D018891:Dentate Gyrus / Q000166:cytology* / Q000201:enzymology; D006358:Hot Temperature; D016857:Hypocapnia / Q000503:physiopathology; D007035:Hypothermia / Q000503:physiopathology*; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012018:Reflex / Q000502:physiology; D013312:Stress, Physiological / Q000503:physiopathology; D014661:Vasoconstriction / Q000502:physiology; D014867:Water
chemical_list: D000963:Antimetabolites; D014867:Water; D019001:Nitric Oxide Synthase; D001973:Bromodeoxyuridine