title: Activation of NF-kappaB and apoptosis of intestinal epithelial cells induced by hydrogen peroxide.
abstract: OBJECTIVE
  In vitro model of hydrogen peroxide induced apoptosis of SW-480 cells was used to investigate the role of NF-kappaB in the pathogenesis of reactive oxygen species induced apoptosis of intestinal epithelial cells.
  
  
  METHODS
  Ultra-structural changes were observed. Apoptosis of SW-480 cell line was determined by Annexin-V and PI double-stained flow cytometry. Nuclear translocation of NF-kappaB was determined by anti-NF-kappaB polyclonal antibody and EB double-staining. NF-kappaB activity was studied by electrophoretic mobility shift assays. RT-PCR was performed to study expression of NF-kappaB mRNA.
  
  
  RESULTS
  Hydrogen peroxide led to apoptosis of SW-480 cells, condensed or semilunar chromatin even apoptotic bodies could be observed. Nuclear translocation of NF-kappaB, increase of NF-kappaB activity and expression of NF-kappaB mRNA were found simultaneously.
  
  
  CONCLUSIONS
  Early activation of NF-kappaB may be one of the mechanisms of apoptosis in intestinal epithelial cells by reactive oxygen species.
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Qian
authors.initials: Q
authors.lastname: Cai
authors.forename: Guangxia
authors.initials: G
authors.lastname: Xiao
mesh_terms: D017209:Apoptosis; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007413:Intestinal Mucosa / Q000378:metabolism*; D018613:Microscopy, Confocal; D016328:NF-kappa B / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000633:toxicity; D014407:Tumor Cells, Cultured
chemical_list: D016328:NF-kappa B; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide