[Dependence of coupling of dopamine receptors with G-proteins on the status of sulfhydryl groups in membrane proteins].
Features of dopamine receptors coupling to G-proteins in the plasma membranes from neural tissue of the mollusc Lymnaea stagnalis were studied. Radioligand binding analysis of [3H]-dopamine and investigation of the GTPase activity have showed that dopamine receptors both inhibit and stimulate coupled G-proteins. It have been found that changing of sulfhydril groups state of the membrane proteins modulates interaction of dopamine receptors with G-proteins. The inhibitory influence of dopamine receptors on the coupled to them G-proteins is potentiated by reduction of thiol groups.
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Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv. malysh@serv.biph.kiev.ua
D000818:Animals; D004298:Dopamine / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008195:Lymnaea; D011485:Protein Binding; D011869:Radioligand Assay; D011954:Receptors, Dopamine / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D011954:Receptors, Dopamine; D013438:Sulfhydryl Compounds; D019204:GTP-Binding Proteins; D004298:Dopamine