Specific fluorescent tracers. Imaging and applications for photodynamic therapy.
Our main objective is to enlarge the fluorescence use in biosciences, with especially the photodynamic therapy (PDT) used for cancer treatment as one of the target applications. Meta-tetra(hydroxyphenyl)chlorin (m-THPC) is a second-generation photosensitiser, applied in photodynamic therapy. The localisation of this sensitiser as well as its induced cell death mechanisms in human breast cancer cells (MCF-7 and its resistant subline MCF-7DXR, DXR: doxorubicin) were evaluated using fluorescence microscopy. In addition, we will present two additional routes, whose aims are to create new features to respond to the PDT questioning: firstly, the synthesis of fluorescent tracers, with a particular attention to the presence of hydrophilic groups (glucosamine ring) on the basic fluorophore structure to orientate the localisation of the probe and, secondly, the use of scanning near-field optical microscopy to reach a better resolution for the fluorescence microscopy analysis.
Teiten
Marie-Hélène
MH
CRAN-IMAC, UMR 7039 CNRS-UHP-INPL, centre Alexis-Vautrin, 54500 Vandoeuvre-les-Nancy, France.
Even
Pascale
P
Burgos
Pierre
P
Frochot
Céline
C
Aubert
Sébastien
S
Carré
Marie-Christiane
MC
Bolotine
Lina
L
Merlin
Jean-Louis
JL
Guillemin
François
F
Viriot
Marie-Laure
ML
D001692:Biological Transport; D001943:Breast Neoplasms / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D004273:DNA, Neoplasm / Q000378:metabolism; D019008:Drug Resistance, Neoplasm; D005260:Female; D005456:Fluorescent Dyes; D006031:Glycosylation; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D008652:Mesoporphyrins / Q000493:pharmacokinetics / Q000633:toxicity; D008856:Microscopy, Fluorescence / Q000379:methods*; D009369:Neoplasms / Q000188:drug therapy; D010778:Photochemotherapy / Q000379:methods*; D011166:Porphyrins / Q000737:chemistry; D011838:Radiation-Sensitizing Agents / Q000493:pharmacokinetics / Q000633:toxicity; D013347:Subcellular Fractions / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; D008652:Mesoporphyrins; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C072269:temoporfin