title: Coupling of the dolichol-P-P-oligosaccharide pathway to translation by perturbation-sensitive regulation of the initiating enzyme, GlcNAc-1-P transferase.
abstract: In mammalian cells, inhibition of translation interferes with synthesis of the lipid-linked oligosaccharide (LLO) Glc3Man9GlcNAc2-P-P-dolichol as measured with radioactive sugar precursors. Conflicting hypotheses have been proposed, and the fundamental basis for this regulation has remained elusive. Here, fluorophore-assisted carbohydrate electrophoresis (FACE) was used to measure LLO concentrations directly in cells treated with translation blockers. Further, LLO biosynthetic enzymes were assayed in vitro with endogenous acceptor substrates using either cells gently permeabilized with streptolysin-O (SLO) or microsomes from homogenized cells. In Chinese hamster ovary (CHO)-K1 cells treated with translation blockers, FACE did not detect changes in concentrations of Glc3Man9GlcNAc2-P-P-dolichol or early LLO intermediates. These results do not support earlier proposals for feedback repression of LLO initiation by accumulated Glc3Man9GlcNAc2-P-P-dolichol, or inhibition of a GDP-mannose dependent transferase. With microsomes from cells treated with translation blockers, there was no interference with LLO initiation by GlcNAc-1-P transferase (GPT), mannose-P-dolichol synthase, glucose-P-dolichol synthase, or LLO synthesis in vitro, as reported previously. Surprisingly, inhibition of all of these was detected with the SLO in vitro system. Additional experiments with the SLO system showed that the three transferases shared a limited pool of dolichol-P that was trapped as Glc3Man9GlcNAc2-P-P-dolichol by translation arrest. Overexpression of GPT was unable to reverse the effects of translation arrest on LLO initiation, and experiments with FACE and the SLO system showed that overexpressed GPT was not functional in vivo, although it was highly active in microsomal assays. Thus, the combined use of the SLO in vitro system and FACE showed that LLO biosynthesis depends upon a limited primary pool of dolichol-P. Physical perturbation associated with microsome preparation appears to make available a secondary pool of dolichol-P, masking inhibition by translation arrest, as well as activating a nonfunctional fraction of GPT. The implications of these results for the organization of the LLO pathway are discussed.
authors.affiliation: Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9041, USA.
authors.forename: Ningguo
authors.initials: N
authors.lastname: Gao
authors.forename: Mark A
authors.initials: MA
authors.lastname: Lehrman
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D016466:CHO Cells; D050260:Carbohydrate Metabolism; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D004286:Dolichols / Q000737:chemistry* / Q000378:metabolism; D006602:Hexosyltransferases; D008070:Lipopolysaccharides / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry; D008565:Membrane Proteins; D008715:Methionine / Q000737:chemistry / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D008954:Models, Biological; D009713:Nucleotidyltransferases / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013301:Streptolysins / Q000494:pharmacology; D013997:Time Factors; D014166:Transferases / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004286:Dolichols; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D009844:Oligosaccharides; D010455:Peptides; D011500:Protein Synthesis Inhibitors; D013301:Streptolysins; C023023:lipid-linked oligosaccharides; C025964:streptolysin O; D003513:Cycloheximide; D008715:Methionine; D014166:Transferases; D006602:Hexosyltransferases; C025620:dolichyl-diphosphooligosaccharide - protein glycotransferase; D009713:Nucleotidyltransferases; C023226:UDPacetylglucosamine pyrophosphorylase; D008358:Mannose