title: Crystallization and preliminary x-ray crystallographic studies of trichosanthin delta C7.
abstract: Trichosanthin (TCS) is a type I ribosome-inactivating protein (RIP) which possesses rRNA N-glycosidase activity. TCS has various pharmacological properties. It is possible to reduce the antigenicity of TCS by deleting up to seven C-terminal residues of TCS (TCS-C7) with minimal effect on its activity. TCS-C7 has been crystallized and the crystal diffracted to 1.8 A. It belongs to space group P2(1), with unit-cell parameters a=71.6A, b=74.4A, c=87.6A, beta=97.0 degrees. It is given that there are four molecules per asymmetric unit.
authors.affiliation: Laboratory of Structural Biology and the MOE Laboratory of Protein Science, School of Life Science & Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Ding
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Dong
authors.forename: P
authors.initials: P
authors.lastname: Shaw
authors.forename: Z
authors.initials: Z
authors.lastname: Rao
authors.forename: H
authors.initials: H
authors.lastname: Too
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D018360:Crystallography, X-Ray; D019662:Cucurbitaceae / Q000378:metabolism; D005822:Genetic Vectors; D006860:Hydrogen Bonding; D017434:Protein Structure, Tertiary; D015978:Trichosanthin / Q000737:chemistry*; D014965:X-Rays
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D015978:Trichosanthin