title: Pathogenicity and virulence of Candida dubliniensis: comparison with C. albicans.
abstract: Candida dubliniensis is a newly described fungus that is frequently isolated from the oral cavities of HIV-positive patients. Although extensive studies have been performed on the phylogeny of C. dubliniensis, little is known about the pathogenic ecology of this yeast. Here we examined aspects related to C. dubliniensis in comparison with those of C. albicans. When injected intravenously into mice, C. dubliniensis had a higher survival rate than C. albicans. Histopathological analysis disclosed that C. dubliniensis remained mostly in the yeast form in the infected organs, whereas C. albicans changed into the mycelial form. The host inflammatory reaction was aggressive with C. dubliniensis infection and mild with C. albicans infection. Co-culture of the yeasts with human polymorphonuclear leukocytes disclosed that C. dubliniensis is more vulnerable to the fungicidal activity of leukocytes than C. albicans. C. dubliniensis was also more susceptible to the toxic effect of hydrogen peroxide. When cultured in vitro, C. dubliniensis grew more slowly than C. albicans, but the formation of germ tubes was faster. When the fungi were cultured in RPMI 1640, a fetal bovine serum supplement suppressed the growth of C. dubliniensis but enhanced that of C. albicans. These results clearly indicated that C. dubliniensis is less virulence than C. albicans.
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Inohana, Japan.
authors.forename: M M S
authors.initials: MM
authors.lastname: Vilela
authors.forename: K
authors.initials: K
authors.lastname: Kamei
authors.forename: A
authors.initials: A
authors.lastname: Sano
authors.forename: R
authors.initials: R
authors.lastname: Tanaka
authors.forename: J
authors.initials: J
authors.lastname: Uno
authors.forename: I
authors.initials: I
authors.lastname: Takahashi
authors.forename: J
authors.initials: J
authors.lastname: Ito
authors.forename: K
authors.initials: K
authors.lastname: Yarita
authors.forename: M
authors.initials: M
authors.lastname: Miyaji
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D002175:Candida / Q000187:drug effects / Q000472:pathogenicity*; D002176:Candida albicans / Q000187:drug effects / Q000472:pathogenicity; D002177:Candidiasis / Q000097:blood / Q000382:microbiology* / Q000473:pathology; D018920:Coculture Techniques; D003470:Culture Media; D004195:Disease Models, Animal; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D051379:Mice; D013045:Species Specificity; D013997:Time Factors; D014774:Virulence
chemical_list: D000891:Anti-Infective Agents, Local; D003470:Culture Media; D006861:Hydrogen Peroxide