Isolation and characterization of Golgi apparatus-specific GODZ with the DHHC zinc finger domain.
We identified a novel Golgi apparatus-specific protein with the DHHC zinc finger domain and four putative transmembrane regions, designated as GODZ. The amino acid sequences were highly conserved among mouse and human GODZs and homologous proteins in human, mouse, rat, Drosophila melanogaster, and Caenorhabditis elegans, implying a functional significance of the GODZ protein family. Overexpression of mouse GODZ in COS7 cells suppressed the sorting of the glutamate receptor GluRalpha1 from the Golgi apparatus. These results suggest that GODZ plays a role in the membrane protein trafficking.
Uemura
Takeshi
T
Department of Molecular Neurobiology and Pharmacology, Graduate School of Medicine, University of Tokyo, and SORST, Japan Science and Technology Corporation, Hongo 7-3-1, Bunkyo-ku, Tokyo, Japan.
Mori
Hisashi
H
Mishina
Masayoshi
M
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D015723:Gene Library; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014018:Tissue Distribution; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers
D008565:Membrane Proteins; D018091:Receptors, AMPA; C465718:GODZ protein, mouse