title: [Multiple FISH and multiple BAND: Application of cytogenetic and molecular techniques in 5 cases].
abstract: The techniques of multiple FISH (M-FISH) or fluorescence in situ hybridization with different color assignment for all and each of the human chromosomes, and multiple BAND (M-BAND) or hybridization with different color assignment for each chromosomal band, allow the identification of some alterations that would not be possible to distinguish with classical banding techniques, like the origin of the chromosomal material that constitutes a (non identifiable) marker chromosome or confirming the constitution of the multiple and simultaneous aberrations that occur in cancer cells. We communicate five complex cytogenetic cases that benefited by the employment of this diagnostic strategy, allowing to corroborate or reformulate a previous given conclusion.
authors.affiliation: Sección Citogenética, Laboratorio Clínica Alemana, Avda. Manquehue 1410, Vitacura, Santiago, Chile. scastill@ns.hospital.uchile.cl
authors.forename: Silvia
authors.initials: S
authors.lastname: Castillo Taucher
authors.forename: Ana María
authors.initials: AM
authors.lastname: Fuentes
authors.forename: Alejandro
authors.initials: A
authors.lastname: Paulos
authors.forename: Andrea
authors.initials: A
authors.lastname: Pardo
mesh_terms: D000328:Adult; D002675:Child, Preschool; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002874:Chromosome Mapping; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007223:Infant; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000235:genetics; D008297:Male; D008875:Middle Aged