Expression and localization of heat shock proteins in rat basophilic leukemia cells: differential modulation by degranulation, thermal or oxidative stress.
BACKGROUND
Rat basophilic leukemia (RBL-2H3) cells are well characterized in terms of morphological and biochemical changes upon activation, and have been extensively used as a model system for studying the mechanisms of the immediate hypersensitivity reaction. To investigate whether overexpression of heat shock/stress proteins (HSP) is involved in the mast cell-dependent reactivity, we examined the adaptive responses of RBL-2H3 cells to classical stress conditions such as heat shock or oxidative injury produced by an aqueous extract of tobacco smoke.


METHODS
HSP were determined by flow cytometry and immunocytochemistry. Degranulation was confirmed as the release of beta-hexosaminidase, determined spectrophotometrically, and by electron microscopy experiments.


RESULTS
We found that RBL-2H3 cells respond to heat shock or oxidative injury by the synthesis of both the inducible 72 kDa HSP (Hsp70), and the oxidation-specific 32 kDa heme oxygenase (HO)-1. Heat shock induced mainly Hsp70 in a cell growth-dependent manner, whereas oxidative stress induced mainly HO-1 in a cell growth-independent manner. However, heat shock or oxidative stress had no significant effects on degranulation.


CONCLUSIONS
Stress-mediated synthesis of HSP was not associated with RBL-2H3 degranulation and likewise, degranulation did not induce HSP.
Bachelet
M
M
Laboratoire de Physiologie Respiratoire et de Biologie Cellulair, UFR Cochin Port-Royal, 24, rue de Faubourg Saint-Jacques, 75014 Paris, France.
Marchand
F
F
Souil
E
E
François
D
D
Mariéthoz
E
E
Weyer
A
A
Polla
B S
BS
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D015550:Cell Degranulation; D002455:Cell Division; D005434:Flow Cytometry; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D051547:Heme Oxygenase-1; D006358:Hot Temperature; D006967:Hypersensitivity / Q000378:metabolism; D007150:Immunohistochemistry; D007476:Ionophores / Q000494:pharmacology; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D014407:Tumor Cells, Cultured; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D007476:Ionophores; D000001:Calcimycin; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D001619:beta-N-Acetylhexosaminidases