title: High metabolic function of primary human and porcine hepatocytes in a polyurethane foam/spheroid culture system in plasma from patients with fulminant hepatic failure.
abstract: It has been demonstrated that plasma from patients with fulminant hepatic failure (FHF) interferes extensively with cellular function. We placed primary human and primary porcine hepatocytes in a polyurethane foam (PUF)/spheroid culture system and compared the metabolic functions in the plasma of patients with FHF in a 24-h stationary culture to those in a monolayer culture. The PUF/spheroid culture system using primary human and primary porcine hepatocytes significantly decreased ammonia content during 28-day culture. Fisher's ratio significantly increased at culture days 3 and 7. Tauroursodeoxycholic acid significantly increased and glycochenodeoxycholic acid and taurochenodeoxycholic acid decreased in the FHF patients' plasma at culture day 3. During at least a 24-h culture in the FHF patients' plasma, metabolic functions of primary human and primary porcine hepatocytes were almost identical. The present results indicate that the PUF/spheroid culture system using primary human or primary porcine hepatocytes demonstrated more advantageous metabolic functions in the plasma from patients with FHF than the monolayer culture.
authors.affiliation: Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. yamashi@surg2.med.kyushu-u.ac.jp
authors.forename: Yo-ichi
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Shimada
authors.forename: Eiji
authors.initials: E
authors.lastname: Tsujita
authors.forename: Ken
authors.initials: K
authors.lastname: Shirabe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ijima
authors.forename: Kohji
authors.initials: K
authors.lastname: Nakazawa
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Sakiyama
authors.forename: Junji
authors.initials: J
authors.lastname: Fukuda
authors.forename: Kazumori
authors.initials: K
authors.lastname: Funatsu
authors.forename: Keizo
authors.initials: K
authors.lastname: Sugimachi
mesh_terms: D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D005999:Glycochenodeoxycholic Acid / Q000097:blood / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D017093:Liver Failure / Q000097:blood* / Q000378:metabolism*; D011140:Polyurethanes / Q000378:metabolism*; D018874:Spheroids, Cellular / Q000378:metabolism*; D013552:Swine; D013655:Taurochenodeoxycholic Acid / Q000097:blood / Q000378:metabolism; D013997:Time Factors
chemical_list: D001647:Bile Acids and Salts; D011140:Polyurethanes; D013655:Taurochenodeoxycholic Acid; C031655:ursodoxicoltaurine; D005999:Glycochenodeoxycholic Acid; D000641:Ammonia; C028279:polyurethane foam