Accumulation of inorganic polyphosphate in phoU mutants of Escherichia coli and Synechocystis sp. strain PCC6803.
The biological process for phosphate (P(i)) removal is based on the use of bacteria capable of accumulating inorganic polyphosphate (polyP). We obtained Escherichia coli mutants which accumulate a large amount of polyP. The polyP accumulation in these mutants was ascribed to a mutation of the phoU gene that encodes a negative regulator of the P(i) regulon. Insertional inactivation of the phoU gene also elevated the intracellular level of polyP in Synechocystis sp. strain PCC6803. The mutant could remove fourfold more P(i) from the medium than the wild-type strain removed.
Morohoshi
Tomohiro
T
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
Maruo
Tatsuya
T
Shirai
Yoko
Y
Kato
Junichi
J
Ikeda
Tsukasa
T
Takiguchi
Noboru
N
Ohtake
Hisao
H
Kuroda
Akio
A
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media; D000458:Cyanobacteria / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D026901:Membrane Transport Proteins; D009154:Mutation; D010710:Phosphates / Q000378:metabolism*; D011122:Polyphosphates / Q000378:metabolism*; D018085:Regulon; D014157:Transcription Factors
D001426:Bacterial Proteins; D003470:Culture Media; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D010710:Phosphates; D011122:Polyphosphates; D014157:Transcription Factors; C050770:phoU protein, E coli