Cytotoxicity and photocytotoxicity of a dendritic C(60) mono-adduct and a malonic acid C(60) tris-adduct on Jurkat cells.
The cytotoxic and photocytotoxic effects of two water-soluble fullerene derivatives, a dendritic C(60) mono-adduct and the malonic acid C(60) tris-adduct were tested on Jurkat cells. Cell growth and vitality were determined by a cell counting and staining technique. After 2 weeks cultivation in the presence of the fullerene derivatives, it was found that only the dendritic mono-adduct inhibits cell growth (within 2 weeks the cell number decreased to 19%), whereas the tris-malonic acid adduct has little effect. The growth inhibition is reversible; cultivating the same cells further in the absence of fullerene, the cell number increased to 106.4%. Other experiments showed that these fullerene derivatives become toxic when irradiated with UVA or UVB light. The cell death is mainly caused by membrane damage and it is UV dose-dependent. Tris-malonic acid fullerene was found to be more phototoxic than the dendritic derivative. This result is in contrast to the singlet oxygen quantum yields determined for the two compounds. We propose that the two fullerene derivatives may interact with the cell membrane in different ways thus causing the observed effects. Further experiments will be done to determine the location and concentration of the two compounds in and on the cells.
Rancan
Fiorenza
F
Department of Dermatology, (Charité) Humboldt University, Berlin, Germany.
Rosan
Stefania
S
Boehm
Fritz
F
Cantrell
Ann
A
Brellreich
Michael
M
Schoenberger
Hubert
H
Hirsch
Andreas
A
Moussa
Fathi
F
D002455:Cell Division; D037741:Fullerenes / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D019169:Jurkat Cells; D008314:Malonates / Q000737:chemistry*; D014466:Ultraviolet Rays
D037741:Fullerenes; D008314:Malonates; C030290:malonic acid; C069837:fullerene C60