title: Failure of Ibuprofen to prevent progressive dermal ischemia after burning in guinea pigs.
abstract: OBJECTIVE
  It is controversial whether the use of prostaglandin inhibitors could prevent progressive dermal ischemia in the postburn stasis zone. This study evaluated the effect of Ibuprofen on preventing postburn dermal ischemia using an animal model of India ink perfusion and skin transparent preparation techniques.
  
  
  METHODS
  The closely clipped backs of the guinea pigs were bathed in 75 degrees C water for 10s. Ibuprofen-treated groups were fed intragastrically with Ibuprofen (12.5mg/kg) every 6h. All animals were perfused with 70% India ink via a cervical artery cannula at 16 kPa constant pressure at 0, 8, 16, 24h postburn. Skin transparent preparations were made, and 6-keto-PGF(1 alpha) and T x B(2) levels in skin tissue were assessed.
  
  
  RESULTS
  India ink filling rates in skin capillary plexuses decreased gradually with postburn time elapsing (P<0.01). 6-keto-PGF(1 alpha) and T x B(2) levels in two groups increased. The increase of T x B(2) was dominant, which was related to postburn dermal ischemia (r=0.742, P<0.01). Though levels of 6-keto-PGF(1 alpha) and T x B(2) decreased in Ibuprofen-treated groups, India ink filling rates showed no significant difference between controls and experimental groups (P>0.05). The results were also confirmed by observation of skin transparent preparations.
  
  
  CONCLUSIONS
  This study suggests that Ibuprofen has no preventive effect on progressive dermal ischemia after burning.
authors.affiliation: Department of Burns and Plastic Surgery, Zhongda Hospital, Southeast University, Nanjing, 210009, Jiangsu, PR China. tanquian@jlonline.com
authors.forename: Qian
authors.initials: Q
authors.lastname: Tan
authors.forename: Zihao
authors.initials: Z
authors.lastname: Lin
authors.forename: Wenxi
authors.initials: W
authors.lastname: Ma
authors.forename: Huairen
authors.initials: H
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Guansen
authors.initials: G
authors.lastname: Ning
authors.forename: Xu
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002056:Burns / Q000150:complications* / Q000473:pathology; D016861:Cyclooxygenase Inhibitors / Q000627:therapeutic use*; D020405:Dermis / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007052:Ibuprofen / Q000627:therapeutic use*; D007511:Ischemia / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control*; D008833:Microcirculation / Q000187:drug effects; D013997:Time Factors; D015599:Trauma Severity Indices; D017211:Treatment Failure
chemical_list: D016861:Cyclooxygenase Inhibitors; D007052:Ibuprofen