title: Pancreatic capillary blood flow in an improved model of necrotizing pancreatitis in the rat.
abstract: BACKGROUND
  The development of acute pancreatitis is characterized by profound changes in pancreatic microcirculation. Using in vivo microscopy with fluorescent-labeled erythrocytes as tracers we studied changes in pancreatic microcirculation in an improved rat model of necrotizing pancreatitis (NP) in comparison to edematous pancreatitis (EP) and healthy controls.
  
  
  METHODS
  Twenty-one male Wistar rats had their pancreatae exteriorized in a temperature-controlled immersion chamber followed by intravenous administration of fluorescent-labeled autologous erythrocytes. EP was induced by intraductal saline and intravenous caerulein (5 microg/kg/h) for 6 h (n = 7) and NP by controlled intraductal infusion of glycodeoxycholic acid (10 mmol/L) followed by intravenous caerulein (n = 7). Control animals received intraductal and intravenous saline (n = 7). The determination of pancreatic microcirculation was performed before as well as 1, 3, and 6 h after intraductal infusion by correlating the number of passing labeled erythrocytes/capillary/min with their concentration per microliter of arterial blood.
  
  
  RESULTS
  Pancreatic capillary flow in control animals remained constant over the 6-h observation period. Pancreatic capillary flow in the EP group rapidly increased to 188% of baseline after 3 h and remained significantly elevated throughout the experiments (P = 0.0001). In contrast, pancreatic capillary flow decreased significantly in the group suffering NP with values 46.7% of baseline after 6 h (P = 0.0001). Complete capillary stasis developed in 38% of investigated capillaries in the NP group compared to 0-1% in both other groups (P = 0.0001).
  
  
  CONCLUSIONS
  Pancreatic microcirculation in mild edematous pancreatitis is significantly increased while the evolution of necrotizing pancreatitis in the model studied herein is characterized by a dramatic reduction in pancreatic capillary flow in conjunction with areas of capillary stasis. These results underline the pathophysiologic relevance of the model and of therapeutic measures aimed at an improvement of pancreatic microcirculation in clinical necrotizing pancreatitis.
authors.affiliation: Department of General Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Schmidt
authors.forename: Dorothea
authors.initials: D
authors.lastname: Ebeling
authors.forename: Eduard
authors.initials: E
authors.lastname: Ryschich
authors.forename: Jens
authors.initials: J
authors.lastname: Werner
authors.forename: Martha M
authors.initials: MM
authors.lastname: Gebhard
authors.forename: Ernst
authors.initials: E
authors.lastname: Klar
mesh_terms: D000818:Animals; D002196:Capillaries / Q000503:physiopathology; D004487:Edema / Q000150:complications; D006439:Hemodynamics; D008297:Male; D010179:Pancreas / Q000098:blood supply*; D010195:Pancreatitis / Q000150:complications / Q000503:physiopathology; D019283:Pancreatitis, Acute Necrotizing / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow