title: Membrane association and kinase-like motifs of the RamC protein of Streptomyces coelicolor.
abstract: The protein RamC is required for the production of the spore-forming cells called aerial hyphae by the filamentous bacterium Streptomyces coelicolor. We showed that RamC, which contains several weakly predicted membrane-spanning sequences, is located exclusively in the S. coelicolor membrane. By constructing site-directed mutants in the cloned ramC gene and complementing a ramC null mutant, we showed that protein kinase-like sequence motifs in the amino-terminal half of the protein are required for function in vivo. These data suggest that RamC is a membrane-associated receptor kinase.
authors.affiliation: Department of Biochemistry and Antimicrobial Research Centre, McMaster University, 200 Main Street West, Hamilton, Ontario L8N 3Z5, Canada.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Hudson
authors.forename: Dachuan
authors.initials: D
authors.lastname: Zhang
authors.forename: Justin R
authors.initials: JR
authors.lastname: Nodwell
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000502:physiology; D008565:Membrane Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000737:chemistry*; D013302:Streptomyces / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D010770:Phosphotransferases