title: Improving the blood compatibility of ion-selective electrodes by employing poly(MPC-co-BMA), a copolymer containing phosphorylcholine, as a membrane coating.
abstract: The hydrogelpoly(2-methacryloyloxyethylphosphorylcholine-co-butyl methacrylate), or poly(MPC-co-BMA), was used as a coating for polyurethane- and poly(vinyl chloride)-based membranes to develop ion-selective electrodes (ISEs) with enhanced blood compatibility. Adverse interactions of poly(MPC-co-BMA) with blood were diminished due to the phosphorylcholine functionalities of the hydrogel, which mimic the phospholipid polar groups present on the surface of many cell membranes. As demonstrated by immunostaining, hydrogel-coated PVC membranes soaked in platelet-rich plasma showed less adhesion and activation of platelets than uncoated PVC membranes, indicating an improvement in biocompatibility owing to the hydrogel. Furthermore, little differences in the potentiometric response characteristics, e.g., slope, detection limit, and selectivity, of ISEs employing uncoated and coated membranes were observed.
authors.affiliation: Department of Chemistry and Center of Membrane Sciences, University of Kentucky, Lexington 40506-0055, USA.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Berrocal
authors.forename: R Daniel
authors.initials: RD
authors.lastname: Johnson
authors.forename: Ibrahim H A
authors.initials: IH
authors.lastname: Badr
authors.forename: Mingdong
authors.initials: M
authors.lastname: Liu
authors.forename: Dayong
authors.initials: D
authors.lastname: Gao
authors.forename: Leonidas G
authors.initials: LG
authors.lastname: Bachas
mesh_terms: D000818:Animals; D001769:Blood / Q000378:metabolism*; D020099:Coated Materials, Biocompatible / Q000592:standards*; D006801:Humans; D020100:Hydrogels; D017736:Ion-Selective Electrodes; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D010973:Platelet Adhesiveness / Q000187:drug effects
chemical_list: D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D008689:Methacrylates; D010767:Phosphorylcholine; C079200:poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate)