title: Excretory/secretory antigens (ES) from in-vitro cultures of Taenia crassiceps cysticerci, and use of an anti-ES monoclonal antibody for antigen detection in samples of cerebrospinal fluid from patients with neurocysticercosis.
abstract: Antigens were obtained from cysticerci of the ORF strain of Taenia crassiceps, by culture of cysts in protein-free hybridoma medium (PFHM). Budding of new vesicles was observed after 24-48 h. Excretory/secretory (ES) antigens (peptides of <20 kDa) were recovered in the medium after culture for 48 h. SDS-PAGE analysis of vesicular-fluid (VF) antigens (obtained by rupturing T. crassiceps cysticerci in PFHM) and the ES antigens indicated partial homology between the two preparations. ES peptides of 18- and 14-kDa were recognized by polyclonal antibodies produced in rabbits immunized either with the VF antigens or with a total-antigen preparation of T. solium cysticerci. Antibodies present in samples of serum or cerebrospinal fluid (CSF) from patients with neurocysticercosis also reacted with ES peptides. An anti-ES monoclonal antibody detected antigens in the CSF from 10 patients with neurocysticercosis, showing the antigenic homology of the ES antigens with those of T. solium cysticerci in human infections.
authors.affiliation: Laboratory of Clinical Immunology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, Bloco 17, 05508-900, São Paulo, SP, Brazil.
authors.forename: N M
authors.initials: NM
authors.lastname: Espíndola
authors.forename: A J
authors.initials: AJ
authors.lastname: Vaz
authors.forename: A X
authors.initials: AX
authors.lastname: Pardini
authors.forename: I
authors.initials: I
authors.lastname: Fernandes
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000134:cerebrospinal fluid*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D020019:Neurocysticercosis / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D013045:Species Specificity; D013621:Taenia / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth