title: Study of the 2719 mutant of the c-H-ras oncogene in a bi-intronic alternative splicing system.
abstract: C-H-ras proto-oncogene forms part of the signal transduction pathway of numerous external stimuli. This proto-oncogene is regulated by alternative splicing within its intron D due to the presence of the alternative intron D exon (IDX). The alternative splicing produces mRNA which encodes for the putative p19 protein, that lacks transforming potential. Herein, we demonstrated that SR proteins regulate the intron D splicing. Moreover, we studied the 2719 mutation of H-ras which has higher transforming potential than Ile12 and Val12 H-ras mutants and is also known to affect the 5' splice site of the IDX. However, here we show that the 2719 mutant can still be spliced when the upstream 5' splice-site is blocked. During these later studies, additionally, we generated a short 11 nucleotides 5' terminal exon that was fully defined and spliced in a bi-intronic pre-mRNA. The definition of this mini-exon was also addressed in this work.
authors.affiliation: IIBB-CSIC (Instituto de Investigaciones Biomédicas de Barcelona-Consejo Superior de Investigaciones Científicas), Dept. PMT, Unidad de Biología y Farmacología Molecular del Cáncer, c/Jorge Girona Salgado 18-26, 08034 Barcelona, Spain.
authors.forename: Sònia
authors.initials: S
authors.lastname: Guil
authors.forename: Edward
authors.initials: E
authors.lastname: Darzynkiewicz
authors.forename: Montse
authors.initials: M
authors.lastname: Bach-Elias
mesh_terms: D017398:Alternative Splicing; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D005091:Exons; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D006801:Humans; D007438:Introns / Q000235:genetics*; D008868:Microtubule Proteins; D009154:Mutation; D010750:Phosphoproteins / Q000235:genetics; D000090063:Proto-Oncogene Mas; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics; D050777:Stathmin
chemical_list: C000715470:MAS1 protein, human; D008868:Microtubule Proteins; D010750:Phosphoproteins; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D017411:Ribonucleoproteins, Small Nuclear; C493995:STMN1 protein, human; D050777:Stathmin; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)