title: [Nested coamplification  polymerase chain reaction].
abstract: OBJECTIVE
  To develop a simple and reliable method for intensifying the hybridization signals of gene chips.
  
  
  METHODS
  The authors added EDTA and another FAM-labeled probe to the normal PCR products, denatured the mixture by heat, and then let the mixture hybridize with the fastened probes on the chip.
  
  
  RESULTS
  With the use of EDTA and another FAM-labeled probe, the hybridization signals increased by 6 times or greater.
  
  
  CONCLUSIONS
  Adding EDTA and another probe to the normal PCR products is a simple and efficient method to intensify the hybridization signal of chips.
authors.affiliation: Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041 P.R.China. szzhang@mcwcms.com
authors.forename: Qingjie
authors.initials: Q
authors.lastname: Xia
authors.forename: Sizhong
authors.initials: S
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Xiujie
authors.initials: X
authors.lastname: Wang
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
mesh_terms: D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004492:Edetic Acid / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D004492:Edetic Acid