The challenges of developing a sound proteomics strategy.
This paper will review the challenges of developing a proteomics strategy. A key issue is the integration of the two-dimensional (2-D) gel platform with mass spectrometry measurements. The use of both matrix-assisted laser/desorption ionization (on off-line coupling) and electrospray (on-line) ionization are complementary. While the use of one-dimensional and 2-D gels are essential to many aspects of proteomics research (sample preparation, preliminary fractionation and quantitation, storage of protein components), the emergence of shotgun sequencing based on high performance liquid chromatography and tandem mass spectrometry offers a powerful new approach. The latter has particular utility in the characterization of low level samples and complex post-translational modifications. The development of capillary columns, such as 75 to 150 micron, that can be packed in a reproducible manner has been a key step in the development of high sensitivity liquid chromatography/mass spectrometry analysis.
Hancock
William S
WS
Thermo Finnigan, San Jose, CA 95134, USA. whancock@thermofinnigan.com
Wu
Shiaw-Lin
SL
Shieh
Paul
P
D002845:Chromatography; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006020:Glycopeptides / Q000032:analysis; D006801:Humans; D040081:Protein Array Analysis; D011506:Proteins / Q000032:analysis; D040901:Proteomics; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006020:Glycopeptides; D011506:Proteins