Biological effects of G1 phase arrest compound, sesquicillin, in human breast cancer cell lines.
Sesquicillin, isolated from fungal fermentation broth, strongly induced G1 phase arrest in human breast cancer cells. During G1 phase arrest, the expression level of cyclin D1, cyclin A, and cyclin E was decreased, and the expression of CDK (cyclin-dependent-kinase) inhibitor, protein p21(Waf1/Cip1), was increased in a time-dependent manner in a breast cancer cell MCF-7. Interestingly, the G1 phase arrest induced by sesquicillin also occurred independently of the tumor suppressor protein, p53. Sesquicillin inhibits the proliferation of MCF-7 via G1 phase arrest in association with the induction of CDK inhibitor protein, p21(Waf1/Cip1), and the reduction of G1 phase related-cyclin proteins.
Jeong
Ha Won
HW
Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yoosung, Taejon, Republic of Korea.
Lee
Ho Jae
HJ
Kho
Yung Hee
YH
Son
Kwang Hee
KH
Han
Mi Young
MY
Lim
Jong Seok
JS
Lee
Mi Young
MY
Han
Dong Cho
DC
Ha
Ji Hong
JH
Kwon
Byoung Mog
BM
D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology*; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005658:Fungi / Q000737:chemistry; D016193:G1 Phase / Q000187:drug effects*; D006801:Humans; D009281:Naphthalenes / Q000302:isolation & purification / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000494:pharmacology
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D009281:Naphthalenes; D016159:Tumor Suppressor Protein p53; C113328:sesquicillin