title: Fine needle aspiration of Langerhans cell histiocytosis of the lymph nodes. A report of six cases.
abstract: BACKGROUND
  Fine needle aspiration (FNA) diagnosis of Langerhans cell histiocytosis (LCH) of the lymph nodes has been described rarely.
  
  
  METHODS
  LCH was confined to the lymph nodes in six children. The FNA smears showed high cellularity composed of many isolated Langerhans cells (LCs) with nuclear grooves and intranuclear inclusions. Also seen were numerous eosinophils, lymphocytes, giant cells, dendriticlike cells, macrophages and Charcot-Leyden crystals in a background of eosinophilic granules. Immunohistochemical study revealed a positive reaction with S-100 protein.
  
  
  CONCLUSIONS
  The presence of LCs with nuclear grooves, eosinophils, giant cells and a positive reaction with S-100 protein aided the diagnosis of LCH of the lymph nodes. Charcot-Leyden crystals, intranuclear inclusions and dendriticlike cells were seen in this study. These findings have not been reported before in lymph node FNA smears of LCH.
authors.affiliation: Department of Pathology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Perikala V
authors.initials: PV
authors.lastname: Kumar
authors.forename: Abdullah
authors.initials: A
authors.lastname: Mousavi
authors.forename: Mehran
authors.initials: M
authors.lastname: Karimi
authors.forename: Gholam Reza
authors.initials: GR
authors.lastname: Bedayat
mesh_terms: D001707:Biopsy, Needle; D002452:Cell Count; D002467:Cell Nucleus / Q000648:ultrastructure; D002648:Child; D004804:Eosinophils / Q000473:pathology*; D006023:Glycoproteins / Q000032:analysis; D006644:Histiocytes / Q000473:pathology*; D006646:Histiocytosis, Langerhans-Cell / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000473:pathology*; D008245:Lysophospholipase; D011110:Polymorphism, Genetic; D009418:S100 Proteins / Q000032:analysis / Q000276:immunology
chemical_list: D006023:Glycoproteins; D009418:S100 Proteins; D008245:Lysophospholipase; C011711:lysolecithin acylhydrolase