Green tea extracts can counteract the modification of fatty acid composition induced by doxorubicin in cultured cardiomyocytes.
Doxorubicin cardiotoxicity is associated with the generation of free radicals, and involves not only lipid peroxidation but also a decreased biosynthesis of highly unsaturated fatty acids, leading to significant modification in cardiomyocyte fatty acid composition. We have evaluated whether naturally occurring antioxidants could counteract this side-effect. Green tea is an excellent source of catechins; we supplemented cultured rat cardiomyocytes with different green tea extracts to relate their catechin content and composition to their ability in protecting cells against doxorubicin-induced damage. The determination of total lipid fatty acid composition, of conjugated diene production (indicator of lipid peroxidation), and of lactate dehydrogenase release revealed that supplementation with tea extracts could counteract significant modifications in the fatty acyl pattern due to doxorubicin exposure, although to different extents. These differences could be ascribed to the different total catechin content and to qualitative differences among the tea extracts, determined by HPLC analysis.
Hrelia
S
S
Nutrition Research Center, Department of Biochemistry G. Moruzzi University of Bologna, Italy. hrelia@biocfarm.unibo.it
Bordoni
A
A
Angeloni
C
C
Leoncini
E
E
Toschi
T Gallina
TG
Lercker
G
G
Biagi
P L
PL
D000466:Alkadienes / Q000032:analysis; D000818:Animals; D002392:Catechin / Q000737:chemistry; D002478:Cells, Cultured; D004317:Doxorubicin / Q000633:toxicity*; D005227:Fatty Acids / Q000378:metabolism*; D006321:Heart / Q000187:drug effects*; D006836:Hydro-Lyases / Q000096:biosynthesis; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D009206:Myocardium / Q000166:cytology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013662:Tea / Q000737:chemistry*
D000466:Alkadienes; D005227:Fatty Acids; D010936:Plant Extracts; D013662:Tea; D004317:Doxorubicin; D002392:Catechin; D006836:Hydro-Lyases; C045581:lactate dehydratase