title: The developments of semisynthetic DNA-protein conjugates.
abstract: Semisynthetic DNA-protein conjugates, generated by either covalent or non-covalent coupling chemistry, are versatile molecular tools applicable in bioanalytical and synthetic chemical procedures. This article reviews the synthesis and characterization of artificial nucleic acid-protein conjugates, in addition to applications arising in the life sciences and nanobiotechnology, such as the self-assembly of high-affinity reagents for immunological detection assays and biosensors, the fabrication of laterally microstructured biochips, and the biomimetic 'bottom-up' synthesis of nanostructured supramolecular devices.
authors.affiliation: Universität Dortmund, Fachbereich Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Str. 6, D-44227 Dortmund, Germany. cmn@chemie.uni-dortmund.de
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
mesh_terms: D001665:Binding Sites; D015374:Biosensing Techniques; D001709:Biotechnology; D004247:DNA / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods; D046911:Macromolecular Substances; D008904:Miniaturization; D036103:Nanotechnology / Q000379:methods* / Q000639:trends; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry
chemical_list: D046911:Macromolecular Substances; D009696:Nucleic Acids; D009841:Oligonucleotides; D011506:Proteins; D004247:DNA; D019809:Streptavidin