Mitochondrial integrity and function in atherogenesis.
BACKGROUND
Coronary atherosclerotic disease remains the leading cause of death in the Western world. Although the exact sequence of events in this process is controversial, reactive oxygen and nitrogen species (RS) likely play an important role in vascular cell dysfunction and atherogenesis. Oxidative damage to the mitochondrial genome with resultant mitochondrial dysfunction is an important consequence of increased intracellular RS.


RESULTS
We examined the contribution of mitochondrial oxidant generation and DNA damage to the progression of atherosclerotic lesions in human arterial specimens and atherosclerosis-prone mice. Mitochondrial DNA damage not only correlated with the extent of atherosclerosis in human specimens and aortas from apolipoprotein E(-/-) mice but also preceded atherogenesis in young apolipoprotein E(-/-) mice. Apolipoprotein E(-/-) mice deficient in manganese superoxide dismutase, a mitochondrial antioxidant enzyme, exhibited early increases in mitochondrial DNA damage and a phenotype of accelerated atherogenesis at arterial branch points.


CONCLUSIONS
Mitochondrial DNA damage may result from RS production in vascular tissues and may in turn be an early event in the initiation of atherosclerotic lesions.
Ballinger
Scott W
SW
Sealy Center for Molecular Cardiology and Division of Cardiology, The University of Texas Medical Branch, Galveston, Tex, USA.
Patterson
Cam
C
Knight-Lozano
Cynthia A
CA
Burow
David L
DL
Conklin
Caryl A
CA
Hu
Zhaoyong
Z
Reuf
Johannes
J
Horaist
Chris
C
Lebovitz
Russell
R
Hunter
Glenn C
GC
McIntyre
Ken
K
Runge
Marschall S
MS
D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000378:metabolism; D004195:Disease Models, Animal; D018450:Disease Progression; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008928:Mitochondria / Q000378:metabolism*; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000172:deficiency / Q000235:genetics; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000096:biosynthesis
D001057:Apolipoproteins E; D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species; C002744:3-nitrotyrosine; D014443:Tyrosine; D013482:Superoxide Dismutase