Crystallization and preliminary x-ray crystallographic studies of trichosanthin delta C7.
Trichosanthin (TCS) is a type I ribosome-inactivating protein (RIP) which possesses rRNA N-glycosidase activity. TCS has various pharmacological properties. It is possible to reduce the antigenicity of TCS by deleting up to seven C-terminal residues of TCS (TCS-C7) with minimal effect on its activity. TCS-C7 has been crystallized and the crystal diffracted to 1.8 A. It belongs to space group P2(1), with unit-cell parameters a=71.6A, b=74.4A, c=87.6A, beta=97.0 degrees. It is given that there are four molecules per asymmetric unit.
Li
X
X
Laboratory of Structural Biology and the MOE Laboratory of Protein Science, School of Life Science & Engineering, Tsinghua University, Beijing 100084, China.
Ding
Y
Y
Wang
Z
Z
Liu
Yi
Y
Dong
Y
Y
Shaw
P
P
Rao
Z
Z
Too
H
H
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D018360:Crystallography, X-Ray; D019662:Cucurbitaceae / Q000378:metabolism; D005822:Genetic Vectors; D006860:Hydrogen Bonding; D017434:Protein Structure, Tertiary; D015978:Trichosanthin / Q000737:chemistry*; D014965:X-Rays
D000972:Antineoplastic Agents, Phytogenic; D015978:Trichosanthin