title: Transformation of Neomycin Resistance Gene (neo(R)) into Silkworm (Bombyx mori.L.).
abstract: Neomycin resistance gene (neo(R)) flanked by 5' and 3' regions of fibroin H-chain gene of silkworm (Bombyx mori.L.) was transferred into eggs of silkworm by gene gun in the early period of fertilization. The larvae were fed with an artificial diet containing neomycin in early 24 hours post transfettion, and some of them survived. The neo(R) encoding sequence in G(2) generation derived from the survivals was detected by Southern blotting. The results indicated that neo(R) could be used as a selective marker for studies on transgenic silkworm.
authors.affiliation: Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. lucd@sunm.shcnc.ac.cn
authors.forename: Xiu
authors.initials: X
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Peng
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Feng
authors.forename: Jun-Ting
authors.initials: JT
authors.lastname: Huang
authors.forename: Chang-De
authors.initials: CD
authors.lastname: Lu