Neurotrophic factors and depolarization do not enhance viability and process regrowth on a purified set of chick embryo retinal ganglion cells.
As retinal ganglion cells (RGCs) develop, the amount of membrane glycoprotein Thy-1 increases, consequently, the RGC binding to Thy-1 antibody enlarges. This phenomenon sustained Thy-1 panning purification of two sets: loose bound cells (LBCs); and tight bound cells (TBCs). Thy-1, neurofilament and growth associated protein-43 characterized both sets as RGCs, but the expression of RA4 antigen and gangliotetraosylgangliosides distinguished LBCs as immature and TBCs as mature. The cell composition in 4-day TBC cultures remained unchanged as immunocharacterization indicated. These cells survived and regrew their processes in plain medium, however, trophic factors (TFs) and depolarization did not enhance their development. This peculiar behavior might be due to their neural maturity, whereas in LBC cultures, TFs produced positive effects.
Brocco
Marcela A
MA
Centro de Investigaciones en Química Biológica de Córdoba, CIQUIBIC, UNC-CONICET, Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, Argentina.
Panzetta
Pedro
P
D000818:Animals; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology; D002642:Chick Embryo; D004594:Electrophysiology; D005136:Eye Proteins / Q000378:metabolism; D019922:GAP-43 Protein / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism; D009414:Nerve Growth Factors / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology*; D016900:Neurofilament Proteins / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000502:physiology*; D018800:Thy-1 Antigens / Q000378:metabolism
D005136:Eye Proteins; D019922:GAP-43 Protein; D005732:Gangliosides; D009414:Nerve Growth Factors; D016900:Neurofilament Proteins; D018800:Thy-1 Antigens