Biochemical Properties of Bound-Ca(2+) in Fibrinolytic Principle (FP) Separated from Agkistrodon acutus Venom.
It has been determined that each FP molecular contains one Ca(2+)-binding site. By the use of fluorescence probe Tb(3+), the distance between Tb(3+) and tryptophan (Trp) residue was obtained to be 0.375. Tb(3+) ion is coordinated with FP more strongly than Ca(2+) ion, and can bind to FP and replace the Ca(2+) ion in FP completely.
Wang
Shou-Ye
SY
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China. lab305@mail.ach.ustc.edu.cn
Zhang
Zu-De
ZD
Chen
Zhen-Long
ZL
Yu
Hua-Ming
HM
Wu
Shuang-Ding
SD
Liu
Qing-Liang
QL