Dioxin catabolic genes are dispersed on the Terrabacter sp. DBF63 genome.
Reverse transcription-PCR of the dbfA1A2, dbfBC, and pht genes, encoding oxygenase component of multicomponent dioxygenase, meta cleavage enzyme and hydrolase, and phthalate-degrading enzymes, respectively, revealed their role in the aromatic compound degradation by Terrabacter sp. strain DBF63. The specific expression in strain DBF63 cells grown on dibenzofuran (the model compound of dioxin; DF) and/or fluorene (FN) indicated that the DbfA1A2 and DbfBC catalyze the conversion of DF to salicylate, and that the DbfA1A2 and Pht enzymes are involved in FN degradation. Pulsed-field gel electrophoresis analyses revealed that the dbfA1A2 cistron and pht operon were located on the two linear plasmids, pDBF1 (160 kb) and pDBF2 (190 kb), while dbfBC genes were located on the chromosome. Because the pht operon is located immediately upstream of the dbfA1A2 cistron, the dioxin-catabolic genes were dispersed on the genome of strain DBF63, while FN-catabolic genes were gathered on the plasmids.
Nojiri
Hideaki
H
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
Kamakura
Mayuko
M
Urata
Masaaki
M
Tanaka
Takahiro
T
Chung
Jin-Sung
JS
Takemura
Tetsuo
T
Yoshida
Takako
T
Habe
Hiroshi
H
Omori
Toshio
T
D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001572:Benzofurans / Q000378:metabolism; D001673:Biodegradation, Environmental; D002273:Carcinogens / Q000378:metabolism; D004147:Dioxins / Q000378:metabolism*; D005449:Fluorenes / Q000378:metabolism; D005798:Genes, Bacterial; D015394:Molecular Structure; D016366:Open Reading Frames; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D010795:Phthalic Acids / Q000378:metabolism; D012988:Soil Microbiology
D001426:Bacterial Proteins; D001572:Benzofurans; D002273:Carcinogens; D004147:Dioxins; D005449:Fluorenes; D010795:Phthalic Acids; C041509:fluorene; C032279:phthalic acid; C023614:dibenzofuran; D010105:Oxygenases