Localization of the antigens D1, D2 and D3 in the rat brain synaptic membrane.
The inside-outside localization of the nervous system specific membrane proteins D1, D2 and D3 was investigated by a immunoabsorption technique. It was found that D1 was located at least partly on the outside of the synaptic membrane in contrast to D3 which was inside on the membrane, facing the cytoplasm. The protein D2 was outside on the synaptic membrane, and it was found very accessible to the antiserum. It is speculated that D2 might be involved in the axonal-dendritic recognition process during synaptogenesis.
Jørgensen
O S
OS
Psychochemistry Institute, K.U.-Rigshospitalet, 9, Blegdamsvej, DK-2100 Copenhagen, Denmark.
D000818:Animals; D000941:Antigens / Q000032:analysis; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D005260:Female; D007106:Immune Sera; D007163:Immunosorbent Techniques; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar; D013570:Synaptic Membranes / Q000737:chemistry*; D013574:Synaptosomes / Q000737:chemistry
D000941:Antigens; D007106:Immune Sera; D009419:Nerve Tissue Proteins; C023513:brain-specific protein D2, rat; C028116:nervous system specific protein D1; C028117:nervous tissue specific protein D3