title: Parameters related to oxygen free radicals in erythrocytes, plasma and epidermis of the hairless rat.
abstract: The following parameters related to oxygen free radicals (OFR) were determined in erythrocytes and the epidermis of hairless rats: catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), reduced (GSH) and oxidized (GSSG) glutathione, glutathione S-transferase (GST), superoxide dismutase (SOD) and thiobarbituric acid reactive substances (TBARS). GSH, GSSG and TBARS were also analyzed in plasma. In erythrocytes, the Pearson correlation coefficients (r) were significant (p < 0.001) between glutathione and other parameters as follows: GSH correlated negatively with GSSG (r = -0.665) and TBARS (r = -0.669); GSSG correlated positively with SOD (r = 0.709) and TBARS (r = 0.752). Plasma GSSG correlated negatively with erythrocytic thermostable GST activity (r = -0.608; p=0.001) and with erythrocytic total GST activity (r = -0.677; p < 0.001). In epidermis (p < 0.001 in all cases), GSH content correlated with GSSG (r = 0.682) and with GPx (r = 0.663); GSSG correlated with GPx (r = 0.731) and with GR (r = 0.794). By multiple linear regression analysis some predictor variables (R(2)) were found: in erythrocytes, thermostable GST was predicted by total GST activity and GSSG, GSSG content was predicted by GSH and by the GSH/GSSG ratio and GPx activity was predicted by GST, CAT and SOD activities; in epidermis, GSSG was predicted by GR and SOD activities and GR was predicted by GSSG, TBARS and GPx. It is concluded that the hairless rat is a good model for studying OFR-related parameters simultaneously in blood and skin, and that it may provide valuable information about other animals under oxidative stress.
authors.affiliation: School of Medicine, University Rovira i Virgili, Sant Llorenç, 21, 43201-REUS, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Romeu
authors.forename: M
authors.initials: M
authors.lastname: Mulero
authors.forename: M
authors.initials: M
authors.lastname: Giralt
authors.forename: J
authors.initials: J
authors.lastname: Folch
authors.forename: M R
authors.initials: MR
authors.lastname: Nogués
authors.forename: A
authors.initials: A
authors.lastname: Torres
authors.forename: A
authors.initials: A
authors.lastname: Fortuño
authors.forename: F X
authors.initials: FX
authors.lastname: Sureda
authors.forename: M
authors.initials: M
authors.lastname: Cabré
authors.forename: J L
authors.initials: JL
authors.lastname: Paternáin
authors.forename: J
authors.initials: J
authors.lastname: Mallol
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002374:Catalase / Q000097:blood / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005609:Free Radicals / Q000097:blood / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000097:blood / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D010100:Oxygen / Q000097:blood / Q000378:metabolism*; D010949:Plasma / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D011923:Rats, Nude; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D010100:Oxygen