title: Optimizing HAPEX topography influences osteoblast response.
abstract: HAPEX (hydroxyapatite-reinforced polyethylene composite) is a second-generation orthopedic biomaterial designed as a bone analog material, which has found clinical success. The use of topography in cell engineering has been shown to affect cell attachment and subsequent response. Thus, by combining bioactivity and enhancing osteoblast response to the implant surface, improved tissue repair and implant life span may be achieved. In this study a primary human osteoblast-like cell model has been used to study the influence of surface topography and chemistry produced by three different production methods. Scanning electron microscopy, fluorescence microscopy, and confocal scanning laser microscopy have been used to study cell adhesion; tritiated thymidine uptake has been used to observe cell proliferation; and the reverse transcriptase-polymerase chain reaction and biochemical methods have been used to study phenotypic expression. Transmission electron microscopy has also been used to look at more long-term morphology. The results show that topography significantly influences cell response, and may be a means of enhancing bone apposition on HAPEX.
authors.affiliation: Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 800, UK. m.dalby@bio.gla.ac.uk
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dalby
authors.forename: Lucy
authors.initials: L
authors.lastname: Di Silvio
authors.forename: Neelam
authors.initials: N
authors.lastname: Gurav
authors.forename: Basil
authors.initials: B
authors.lastname: Annaz
authors.forename: Michael V
authors.initials: MV
authors.lastname: Kayser
authors.forename: William
authors.initials: W
authors.lastname: Bonfield
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D017886:Durapatite; D006801:Humans; D008422:Materials Testing; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D011095:Polyethylenes; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D011095:Polyethylenes; D003094:Collagen; D017886:Durapatite; D000469:Alkaline Phosphatase