title: Expression of Brain-derived Neurotrophic Factor (BDNF) Gene in Rat Myoblast Cells.
abstract: Brain-derived neurotrophic factor (BDNF) gene was cloned into retrovirous vector plasmid pLNCX to form the pLNC/BDNF. After packaging in PA317 cell line, the infectious particles were used to infect rat myoblast cell line L-6TG. After selection with G418, the L-6TG/BDNF cells were collected. Results from Southern blot show that bdnf gene has successfully integrated into the L-6TG genomic DNA. The expression of bdnf gene has been proved by Northern blot and Dot blot. The content of BDNF in the culture medium of L-6TG/BDNF was about 25 ng.10(-6) cells per 24 h per ml.
authors.affiliation: Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. sib203@sunm.shcnc.ac.cn
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Jiang
authors.forename: Kai
authors.initials: K
authors.lastname: Ge
authors.forename: Lan-Ying
authors.initials: LY
authors.lastname: Sun
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Liu
authors.forename: Zhong-Cheng
authors.initials: ZC
authors.lastname: Zheng