title: Cloning and Expression of a Fused Gene Encoding TNFRI Death Domain and Chloramphenicol Acetyltransferase.
abstract: By designing four primers for an overlapping PCR, we created a fusion gene Ddlcat encoding human TNF receptor I death domain and chloramphenicol acetyltransferase (CAT). By DNA sequencing, the whole sequence of the fusion gene is confirmed to be correct. Two hours after induction with IPTG, we could see a 39 kD extra protein band on SDS-PAGE pattern. We proved that this 39 kD protein band is DdLcat protein by Western blotting. Then we purified this protein to the purity of 95% through Q-Sepharose chromatography.
authors.affiliation: Institute of Genetics, Fudan University, State Key Laboratory of Genetic Engineering, Shanghai 200433, China. shouyuanzhao@fudan.edu.cn
authors.forename: Li-Zhu
authors.initials: LZ
authors.lastname: Lin
authors.forename: Chang-Ben
authors.initials: CB
authors.lastname: Li
authors.forename: Shou-Yuan
authors.initials: SY
authors.lastname: Zhao
authors.forename: G.
authors.initials: G
authors.lastname: De Wilde
authors.forename: G.
authors.initials: G
authors.lastname: Haegeman
authors.forename: W.
authors.initials: W
authors.lastname: Fiers