Real-time PCR detection of salmonella in suspect foods from a gastroenteritis outbreak in kerr county, Texas.
In June 2001, an outbreak of acute gastroenteritis among 109 attendees of a church picnic in Kerr County, Texas, was reported. A 5'-nuclease PCR assay was used to screen for Salmonella in nine food items from the buffet line. Barbeque chicken B tested positive for Salmonella, and no amplification was detected in the remaining food items. These PCR findings were consistent with culture results and were confirmed by direct nucleotide sequencing. Salmonella enterica serotype Panama was cultured from both food and patient stool samples.
Daum
Luke T
LT
Brooks Air Force Base, San Antonio, Texas 78235-5237, USA. Luke.Daum@brooks.af.mil
Barnes
William J
WJ
McAvin
James C
JC
Neidert
Margaret S
MS
Cooper
Lynn A
LA
Huff
William B
WB
Gaul
Linda
L
Riggins
W S
WS
Morris
Sandra
S
Salmen
Ann
A
Lohman
Kenton L
KL
D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012478:Salmonella Food Poisoning / Q000453:epidemiology* / Q000382:microbiology; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013781:Texas / Q000453:epidemiology
D004269:DNA, Bacterial