Establishment of the telencephalon during gastrulation by local antagonism of Wnt signaling.
Cells at the anterior boundary of the neural plate (ANB) can induce telencephalic gene expression when transplanted to more posterior regions. Here, we identify a secreted Frizzled-related Wnt antagonist, Tlc, that is expressed in ANB cells and can cell nonautonomously promote telencephalic gene expression in a concentration-dependent manner. Moreover, abrogation of Tlc function compromises telencephalic development. We also identify Wnt8b as a locally acting modulator of regional fate in the anterior neural plate and a likely target for antagonism by Tlc. Finally, we show that tlc expression is regulated by signals that establish early antero-posterior and dorso-ventral ectodermal pattern. From these studies, we propose that local antagonism of Wnt activity within the anterior ectoderm is required to establish the telencephalon.
Houart
Corinne
C
MRC Centre for Developmental Neurobiology, New Hunt's House, King's College London, SE1 9RT, London, United Kingdom. corinne.houart@kcl.ac.uk
Caneparo
Luca
L
Heisenberg
Carl
C
Barth
K
K
Take-Uchi
Masaya
M
Wilson
Stephen
S
D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D003598:Cytoskeletal Proteins; D003714:Denervation; D005775:Gastrula / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010802:Phylogeny; D016548:Prosencephalon / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D013687:Telencephalon / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D016212:Transforming Growth Factor beta; D051153:Wnt Proteins; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000302:isolation & purification*; D000099274:Frizzled-Related Proteins
D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D003598:Cytoskeletal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D016212:Transforming Growth Factor beta; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D000099274:Frizzled-Related Proteins; C525826:bmp7a protein, zebrafish; C094521:wnt8a protein, zebrafish; C094522:wnt8b protein, zebrafish