title: [Two cases of acute lymphoblastic leukemia with cytoplasmic granules].
abstract: The presence of cytoplasmic granules in blastoid cells of patients with acute leukemia is generally accepted as a useful morphological marker for differentiation of myeloid leukemia from lymphoblastic leukemia. We diagnosed two cases of acute lymphoblastic leukemia(ALL) with cytoplasmic granulation. Surface marker analysis of leukemic cells revealed they were positive for CD10, 19, 20, 33, 34 and HLA-DR. Immunoglobulin gene rearrangement was detected by means of Southern hybridization with an Ig-JH probe for both patients. On the basis of these findings, the patients were diagnosed as having B-precursor ALL. Electron microscopic observation showed no myeloperoxidase activity, so that the granules were considered to be related to autophagolysosomes. This experience demonstrates that the recognition of the presence of granular ALL is necessary for making an accurate differential diagnosis of acute leukemias.
authors.affiliation: Blood Transfusion Division, Kobe University Hospital, Kobe 650-0017.
authors.forename: Shiho
authors.initials: S
authors.lastname: Morita
authors.forename: Katsuyasu
authors.initials: K
authors.lastname: Saigo
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kosaka
authors.forename: Kimihiko
authors.initials: K
authors.lastname: Sano
authors.forename: Eiji
authors.initials: E
authors.lastname: Tatsumi
authors.forename: Shion
authors.initials: S
authors.lastname: Imoto
authors.forename: Daiki
authors.initials: D
authors.lastname: Shimomura
authors.forename: Shiomi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Tadanobu
authors.initials: T
authors.lastname: Chinzei
mesh_terms: D002648:Child; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology*