title: Helicobacter pylori SabA adhesin in persistent infection and chronic inflammation.
abstract: Helicobacter pylori adherence in the human gastric mucosa involves specific bacterial adhesins and cognate host receptors. Here, we identify sialyl-dimeric-Lewis x glycosphingolipid as a receptor for H. pylori and show that H. pylori infection induced formation of sialyl-Lewis x antigens in gastric epithelium in humans and in a Rhesus monkey. The corresponding sialic acid-binding adhesin (SabA) was isolated with the "retagging" method, and the underlying sabA gene (JHP662/HP0725) was identified. The ability of many H. pylori strains to adhere to sialylated glycoconjugates expressed during chronic inflammation might thus contribute to virulence and the extraordinary chronicity of H. pylori infection.
authors.affiliation: Department of Odontology/Oral Microbiology, Umeå University, SE-901 87 Umeå, Sweden.
authors.forename: Jafar
authors.initials: J
authors.lastname: Mahdavi
authors.forename: Berit
authors.initials: B
authors.lastname: Sondén
authors.forename: Marina
authors.initials: M
authors.lastname: Hurtig
authors.forename: Farzad O
authors.initials: FO
authors.lastname: Olfat
authors.forename: Lina
authors.initials: L
authors.lastname: Forsberg
authors.forename: Niamh
authors.initials: N
authors.lastname: Roche
authors.forename: Jonas
authors.initials: J
authors.lastname: Angstrom
authors.forename: Thomas
authors.initials: T
authors.lastname: Larsson
authors.forename: Susann
authors.initials: S
authors.lastname: Teneberg
authors.forename: Karl-Anders
authors.initials: KA
authors.lastname: Karlsson
authors.forename: Siiri
authors.initials: S
authors.lastname: Altraja
authors.forename: Torkel
authors.initials: T
authors.lastname: Wadström
authors.forename: Dangeruta
authors.initials: D
authors.lastname: Kersulyte
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Berg
authors.forename: Andre
authors.initials: A
authors.lastname: Dubois
authors.forename: Christoffer
authors.initials: C
authors.lastname: Petersson
authors.forename: Karl-Eric
authors.initials: KE
authors.lastname: Magnusson
authors.forename: Thomas
authors.initials: T
authors.lastname: Norberg
authors.forename: Frank
authors.initials: F
authors.lastname: Lindh
authors.forename: Bertil B
authors.initials: BB
authors.lastname: Lundskog
authors.forename: Anna
authors.initials: A
authors.lastname: Arnqvist
authors.forename: Lennart
authors.initials: L
authors.lastname: Hammarström
authors.forename: Thomas
authors.initials: T
authors.lastname: Borén
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001422:Bacterial Adhesion; D002240:Carbohydrate Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D005753:Gastric Mucosa / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D005756:Gastritis / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D005798:Genes, Bacterial; D006001:Glycoconjugates / Q000378:metabolism; D016481:Helicobacter Infections / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D016256:Lewis X Antigen / Q000378:metabolism*; D008253:Macaca mulatta; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism; D000080506:Sialyl Lewis X Antigen
chemical_list: D018829:Adhesins, Bacterial; C400429:BabA protein, Helicobacter pylori; D002352:Carrier Proteins; D006001:Glycoconjugates; D016256:Lewis X Antigen; D009844:Oligosaccharides; D012794:Sialic Acids; D000080506:Sialyl Lewis X Antigen; C404741:galactopyranosyl-1-3-galactopyranosyl-1-3(4)-N-acetylglucosamine