Amplification and expression of genes from the 17q11 approximately q12 amplicon in breast cancer cells.
Amplification of a portion of 17q11 approximately q12 involving the human epidermal growth factor receptor-2 (HER-2/neu) gene is common in solid tumors. HER-2/neu amplification in breast cancer is associated with a poor prognosis and may predict response to therapeutic interventions. The molecular complexity and informative content of the 17q11 approximately q12 amplified DNA remain largely unknown. Study of available sequence information in public database allowed us to construct a contig in bacterial artificial chromosome that covers this region. We have identified a several hundred kilobase core segment from this region that is amplified in three out of three breast cancer cell lines with HER-2/neu amplification. There is striking correlation between amplification and overexpression of genes from this region. Amplified and overexpressed genes, in addition to HER-2/neu, may play functional roles in the pathogenesis of breast cancer and may serve as additional targets for therapy. Published by Elsevier Science Inc.
Luoh
Shiuh-Wen
SW
Department of Internal Medicine, Division of Hematology and Oncology, University of Cincinnati, College of Medicine, The Vontz Center for Molecular Studies, 3125 Eden Avenue, Cincinnati, OH 45267-0508, USA. shiuh-wen.luoh@uc.edu
D001943:Breast Neoplasms / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial; D002886:Chromosomes, Human, Pair 17; D020451:Contig Mapping; D005260:Female; D005784:Gene Amplification; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D056892:Mediator Complex; D056913:Mediator Complex Subunit 1; D014157:Transcription Factors / Q000235:genetics
D002352:Carrier Proteins; C538855:MED1 protein, human; C503845:MED24 protein, human; D056892:Mediator Complex; D056913:Mediator Complex Subunit 1; D014157:Transcription Factors