[Retrotransposon mdg3 is transferred between somatic cells of unrelated species in coculture].
Since retrovirus-like particles of gypsy (mdg4) are capable of interspecific transfer, other Drosophila melanogaster gypsy-related retrotransposons were tested for this property. As a donor and a recipient, D. melanogaster and D. virilis cultured cells were used. Recipient cell DNA was analyzed with probes directed to mdg1, mdg3, 17.6, 297, 412, or B104/roo. Transfer was demonstrated for mdg3, which lacks env. The possible mechanism of transfer is discussed.
Semin
B V
BV
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia. syomin@genome.eimb.relarn.ru
Leonova
T Ia
TIa
Il'in
Iu V
IuV
D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D004330:Drosophila / Q000166:cytology / Q000235:genetics*; D022761:Gene Transfer, Horizontal; D015752:Genes, env; D018626:Retroelements
D018626:Retroelements