Expression of beta-defensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells.
Human beta-defensins are broad-spectrum antimicrobial peptides known to be produced by epithelial cells. It was recently shown that beta-defensins also display chemotactic activity for dendritic cells (DC) and T cells, and thus may serve to link innate and adaptive immunity. The aim of the present study was to explore expression of mRNA for these peptides in mononuclear phagocytes and DC. The results revealed that monocytes, monocyte-derived-macrophages (MDM), and monocyte-derived-dendritic cells (DC) all express human-beta-defensin-1 (hBD-1) mRNA. hBD-1 mRNA expression by monocytes and MDM was increased after activation with interferon-gamma (IFN-gamma) and/or lipopolysaccharide (LPS) in a dose- and time-dependent fashion. Alveolar macrophages showed an intense hBD-1 expression, which could not be further increased. Expression of hBD-1 mRNA by immature DC was low, and increased considerably after maturation. Monocytes, MDM, alveolar macrophages and DC showed a limited expression of human beta-defensin-2 (hBD-2) mRNA, which could only be increased in monocytes and alveolar macrophages by IFN-gamma and/or LPS in a dose- and time-dependent fashion. Immunocytochemical stainings demonstrated the expression of hBD-2 peptide by freshly isolated blood monocytes and alveolar macrophages in cytospin preparations.
Duits
Louise A
LA
Departments of Infectious Diseases and Pulmonology, Leiden University Medical Center, Leiden, The Netherlands.
Ravensbergen
Bep
B
Rademaker
Mirjam
M
Hiemstra
Pieter S
PS
Nibbering
Peter H
PH
D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D015870:Gene Expression; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D016676:Macrophages, Alveolar / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D010586:Phagocytes / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023083:beta-Defensins / Q000235:genetics / Q000378:metabolism*
C406407:DEFB1 protein, human; C106641:DEFB4A protein, human; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D023083:beta-Defensins; D007371:Interferon-gamma