Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage.
By homologous recombination of an internal ribosome entry site and Cre recombinase coding region into the 3'-untranslated region of the mouse Emx1 gene, we have generated a strain of mice, Emx1(IRES)cre, that expresses the Cre recombinase in a spatial and temporal pattern like that observed for Emx1. When mated to reporter strains, these mice are a sensitive means to fate-map the Emx1-expressing cells of the developing forebrain. Our results demonstrate that radial glia, Cajal-Retzius cells, glutamatergic neurons, astrocytes, and oligodendrocytes of most pallial structures originate from an Emx1-expressing lineage. On the other hand, most of the pallial GABAergic neurons arise outside the Emx1-expressing lineage. Structures that are located near the basal ganglia (e.g., the amygdala and endopiriform nuclei) are not uniformly derived from Emx1-expressing cells.
Gorski
Jessica A
JA
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA.
Talley
Tiffany
T
Qiu
Mengsheng
M
Puelles
Luis
L
Rubenstein
John L R
JL
Jones
Kevin R
KR
D020413:3' Untranslated Regions / Q000235:genetics; D000679:Amygdala / Q000166:cytology / Q000196:embryology; D000818:Animals; D001479:Basal Ganglia / Q000166:cytology / Q000196:embryology; D019070:Cell Lineage; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology; D018390:Gene Targeting; D017930:Genes, Reporter; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D019426:Integrases / Q000096:biosynthesis / Q000235:genetics; D007395:Interneurons / Q000166:cytology; D051379:Mice; D008817:Mice, Mutant Strains; D023421:Models, Animal; D009457:Neuroglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D011995:Recombination, Genetic; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013687:Telencephalon / Q000166:cytology* / Q000196:embryology*; D014157:Transcription Factors; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*; D000099260:Empty Spiracles Homeobox Proteins
D020413:3' Untranslated Regions; D018398:Homeodomain Proteins; D019426:Integrases; D014157:Transcription Factors; D014764:Viral Proteins; D005680:gamma-Aminobutyric Acid; D000099260:Empty Spiracles Homeobox Proteins; C045073:Cre recombinase