title: The CD19 compartment in myeloma includes a population of clonal cells persistent after high-dose treatment.
abstract: Peripheral blood-localized clonal cells in patients with multiple myeloma (MM) have been reported to be insensitive to chemotherapy and it has been suggested that these cells may include the proliferative compartment in myeloma. In this study, circulating clonal CD19+ cells levels were determined in 10 patients with MM by performing patient-specific RT-PCR on single flow-sorted CD19+ cells. A variable proportion of CD+ cells being clonal were identified (0.101-6.130%, mean 1.019%). In 10/10 MM patients clonal CD19+ cells were found after high-dose treatment and peripheral blood stem cell transplantation. In conclusion, CD19+ clonal cells include a population of cells persistent after high dose chemotherapy that may be responsible for relapse.
authors.affiliation: Department of Hematology, Herlev Hospital, University of Copenhagen, Herlev, Denmark. thra@herlevhosp.kbhamt.dk
authors.forename: Thomas
authors.initials: T
authors.lastname: Rasmussen
authors.forename: Linda
authors.initials: L
authors.lastname: Jensen
authors.forename: Hans Erik
authors.initials: HE
authors.lastname: Johnsen
mesh_terms: D018941:Antigens, CD19 / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D015321:Gene Rearrangement; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D009101:Multiple Myeloma / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018941:Antigens, CD19; D007143:Immunoglobulin Heavy Chains