Embryonic expression of mSharp-1/mDEC2, which encodes a basic helix-loop-helix transcription factor.
We describe the expression pattern of mouse Sharp-1 (mSharp-1), a member of the basic helix-loop-helix (bHLH) family of transcription factors. mSharp-1 belongs to the Hairy/Enhancer of Split (E(Spl)) subfamily of bHLH factors that are key targets of the Notch signaling pathway and exhibits the highest sequence identity with Stra13. RNA in situ hybridization analysis from embryonic day 7.5 (E7.5) to E16.5 revealed specific expression of mSharp-1 in several developing organs during mouse embryogenesis. In early stage embryos (E8.5-E12.5), mSharp-1 is expressed in specific dorsal regions of the developing brain, the heart, the developing eye and olfactory system, as well as in the limb buds. At later stages (E12.5-E16.5), mSharp-1 is also expressed in the liver, prevertebrae, and the developing adrenal and thyroid glands. The diversity of its expression pattern suggests that mSharp-1 may regulate the differentiation of several cell types during vertebrate development.
Azmi
Sameena
S
Department of Biochemisty and Molecular Biology, Mount Sinai School of Medicine, Box 1020, One Gustave L. Levy Place, New York, NY 10029-6574, USA.
Taneja
Reshma
R
D000818:Animals; D002454:Cell Differentiation; D018076:DNA, Complementary / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D018257:Helix-Loop-Helix Motifs; D017403:In Situ Hybridization; D051379:Mice; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics*
C470968:Bhlhb3 protein, mouse; D018076:DNA, Complementary; D012333:RNA, Messenger; D014157:Transcription Factors; D012313:RNA