Dendritic cells and dendritic-like microglia in focal cortical ischemia of the mouse brain.
Intracerebral dendritic cells (DC) have recently been identified in neuroinflammation initiated peripherally by brain-targeted autoimmunity or infection. The present study detects DC in photochemically induced cortical ischemia of the mouse brain, a brain-intrinsic lesion model characterized by the lack of an overt T cell response. Concomitant to leukocyte infiltration of the infarcted area, cells expressing the pan-DC surface marker CD11c appeared at the lesion and persisted for weeks. These DC were located at the border zone of the infarct and remote from the lesion in degenerating corticothalamic fibre tracts and subcortical nuclei. All CD11c+ brain cells displayed a uniform CD11b+/CD8alpha-/CD205- surface phenotype, indicating a myeloid origin, and were immature DC based on their MHC class II+/CD40-/CD80+/CD86+/- profile. By expressing high levels of CD45, most DC from ischemic brain seemed to be blood-derived while a minority were CD45(low), thus corresponding to resident microglia. Consistently, round-shaped CD11c+ cells were found at the lesion whereas CD11c+ cells at subcortical sites were ramified like parenchymal microglia. These findings evidence a recruitment of myeloid DC to ischemic brain lesions and suggest that reactive microglia in remote areas transform into dendritic-like cells. Brain-infiltrating DC and their microglial counterparts may play a role in the inflammatory response to cerebral ischemia independently of T cells.
Reichmann
Gaby
G
Institute for Medical Microbiology and Virology, Heinrich-Heine-University, Universitätsstrasse 1, D-40225, Düsseldorf, Germany.
Schroeter
Michael
M
Jander
Sebastian
S
Fischer
Hans-Georg
HG
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D018122:B7-1 Antigen / Q000276:immunology; D002545:Brain Ischemia / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D017252:CD3 Complex / Q000276:immunology; D002540:Cerebral Cortex / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004195:Disease Models, Animal; D004660:Encephalitis / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D016167:Integrin alphaXbeta2 / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000166:cytology / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
D000954:Antigens, Surface; D018122:B7-1 Antigen; D017252:CD3 Complex; D000949:Histocompatibility Antigens Class II; D016167:Integrin alphaXbeta2; D017493:Leukocyte Common Antigens