title: Test characteristics of the respiratory syncytial virus enzyme-linked immunoabsorbent assay in febrile infants < or = 60 days of age.
abstract: The test characteristics of rapid tests for respiratory syncytial virus (RSV) in infants may differ from older children secondary to a lower likelihood of previous illness with RSV. Our main goal was to establish the test characteristics of the RSV Abbott Testpack (TP) enzyme-linked immunoabsorbent assay (EIA) in febrile infants < or = 60 days of age. Our secondary goal was to determine the likelihood of RSV given a particular clinical syndrome and a negative or positive EIA. A prospective sample of infants with a temperature > or = 38.0 degrees C was evaluated during 2 successive RSV seasons. Conventional tissue and shell vial viral cultures were utilized as the reference standard. The RSV Abbott Testpack EIA had a sensitivity of 75% (95% CI 60-90%), a specificity of 98% (95% CI 96-100%), a positive predictive value of 89% (95% CI 77-100%), a negative predictive value of 95% (95% CI 91-98%), a likelihood ratio for a positive test of 35.5 (95% CI 11.4-110.7), and a likelihood ratio for a negative test of 0.26 (95% CI 0.14-0.47). Even with a negative EIA, patients with lower and upper respiratory tract illness still had a 22.3% and 5.5% chance of harboring RSV, respectively. The RSV Abbott Testpack is a useful diagnostic tool in the detection of RSV in febrile infants but has limitations. During months typically associated with RSV disease, a positive RSV TP indicates a high likelihood of illness, but clinicians should be wary of false negatives.
authors.affiliation: Children's Hospital of New York, Columbia University College of Physicians and Surgeons, New York, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Dayan
authors.forename: Faiz
authors.initials: F
authors.lastname: Ahmad
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Urtecho
authors.forename: Michael
authors.initials: M
authors.lastname: Novick
authors.forename: Patricia
authors.initials: P
authors.lastname: Dixon
authors.forename: Debbie
authors.initials: D
authors.lastname: Levine
authors.forename: Steven
authors.initials: S
authors.lastname: Miller
mesh_terms: D002675:Child, Preschool; D016001:Confidence Intervals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D011237:Predictive Value of Tests; D011446:Prospective Studies; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification*; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index