title: Structural characterization of the second TSP1-module of human thrombospondin.
abstract: The TSP1-module has been first identified as the type 1 repeat of thrombospondin-1. Members of this extracellular module-family have since been shown to be present in several hundred metazoan proteins as well as in proteins of some protists. Despite the widespread occurrence and biological importance of this module-type, relatively little is known about their three-dimensional structure. To define the structural features of this important module-family, we have expressed the second TSP1-domain of human thrombospondin 1 in Escherichia coli. Amino acid sequencing of proteolytic fragments of the recombinant protein have shown that its disulfide bonds connect the six conserved cysteines in a 1-5, 2-6, 3-4 pattern. Circular dichroism studies on the recombinant protein indicate that the disulfide-bonded TSP1-module consists primarily of distorted beta-strands.
authors.affiliation: Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H-1113, Karolina ut 29, P.O. Box 7, H-1518, Budapest, Hungary.
authors.forename: Emoke
authors.initials: E
authors.lastname: Roszmusz
authors.forename: András
authors.initials: A
authors.lastname: Patthy
authors.forename: Mária
authors.initials: M
authors.lastname: Trexler
authors.forename: László
authors.initials: L
authors.lastname: Patthy
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002942:Circular Dichroism; D003001:Cloning, Molecular; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013056:Spectrophotometry, Ultraviolet; D019699:Thrombospondins / Q000737:chemistry* / Q000235:genetics
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; D019699:Thrombospondins