title: Reperfusion injury associated with portal venous and hepatic arterial perfusion in liver transplantation.
abstract: BACKGROUND
  Although reperfusion injury in organ transplantation is presently well established, its exact role in liver transplantation still has to be defined. The aim of this part of the study was to document the reperfusion injury associated with porcine liver transplantation and to evaluate the different components of the reperfusion injury associated with arterial and portal reperfusion.
  
  
  METHODS
  Large white X Landrace pigs were randomized into two groups: group 1, initial portal reperfusion, and group 2, initial arterial reperfusion. Several indicators of reperfusion injury, endothelial cell function, and hepatocellular damage were assessed. Early histopathological findings in biopsy specimens can predict poor graft outcome, therefore histological findings of the liver biopsies after liver transplantation were also studied.
  
  
  RESULTS
  Malondialdehyde concentrations were lower and vitamin A concentrations were higher in the animals subjected to initial portal reperfusion. Serum amino aspartate transferase and serum hyaluronic acid concentrations were higher in the animals subjected to initial portal reperfusion. Histological results showed that hepatocyte vacuolization, neutrophil infiltration, single hepatocyte necrosis, and group cell necrosis of the hepatocytes were all significantly reduced in group 2 compared to group 1.
  
  
  CONCLUSIONS
  Results of this study indicate that the major part of reperfusion injury is constituted during the portal venous reperfusion and that this injury can be, at least partially, attenuated by initial arterial reperfusion.
authors.affiliation: Department of Surgery, and the Medical Research Council, Liver Research Centre, University of Cape Town, Cape Town, South Africa. abvanas@pawc.wcape.gov.za.
authors.forename: Arjan B
authors.initials: AB
authors.lastname: van As
authors.forename: Zoe
authors.initials: Z
authors.lastname: Lotz
authors.forename: Marilyn
authors.initials: M
authors.lastname: Tyler
authors.forename: Delawir
authors.initials: D
authors.lastname: Kahn
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D005260:Female; D006499:Hepatic Artery / Q000502:physiology*; D006820:Hyaluronic Acid / Q000097:blood; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D010477:Perfusion; D011169:Portal Vein / Q000502:physiology*; D015427:Reperfusion Injury / Q000209:etiology*; D013552:Swine; D014801:Vitamin A / Q000097:blood
chemical_list: D014801:Vitamin A; D008315:Malondialdehyde; D006820:Hyaluronic Acid; D000410:Alanine Transaminase