Phospholipid composition and [14C]glycerol incorporation into glycerolipids of toad retina and brain.
The phospholipid composition as well as the in vivo [14C]glycerol uptake in lipids was found to be similar in the toad brain and retina. The choroid lipid labeling was markedly different. An in vitro time-course study of [14C]glycerol incorporation in toad retina lipids disclosed that under the conditions of these experiments: (1) retina is able to rapidly synthesize phosphatidic acid from the radioactive precursor; (2) the sequence phosphatidic acid-diacylglycerol-triacylglycerol operates; (3) a high rate of phosphatidylinositol de novo biosynthesis takes place; (4) phosphoglycerides of choline and of ethanolamine are also heavily labeled after a lag period; (5) in vivo labeling profiles resembled those obtained in vitro mainly regarding phosphatidylinositol biosynthesis; and (6) the presence of glycerol kinase in the CNS is suggested.
Pascual de Bazán
H E
HE
Instituto de Investigaciones Bioquímicas, Universidad Nacional del Sur Consejo Nacional de Investigaciones Cientificas y Técnicas, 8000 Bahía Blanca, Argentina.
Bazán
N G
NG
D000818:Animals; D001923:Brain Chemistry; D002022:Bufo arenarum; D002250:Carbon Radioisotopes; D002829:Choroid / Q000737:chemistry / Q000378:metabolism; D004075:Diglycerides / Q000096:biosynthesis; D005260:Female; D005989:Glycerides / Q000096:biosynthesis*; D005990:Glycerol / Q000378:metabolism* / Q000493:pharmacokinetics; D066298:In Vitro Techniques; D008297:Male; D010712:Phosphatidic Acids / Q000096:biosynthesis; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010714:Phosphatidylethanolamines / Q000096:biosynthesis; D010716:Phosphatidylinositols / Q000096:biosynthesis; D010743:Phospholipids / Q000032:analysis*; D012160:Retina / Q000737:chemistry / Q000378:metabolism*; D014280:Triglycerides / Q000096:biosynthesis
D002250:Carbon Radioisotopes; D004075:Diglycerides; D005989:Glycerides; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010743:Phospholipids; D014280:Triglycerides; D005990:Glycerol