Aquaporin-2 transcript is differentially regulated by dietary salt in Sprague-Dawley and Dahl SS/Jr rats.
Aquaporin-2 (AQP-2) is a vasopressin-regulated water channel in the kidney collecting duct. AQP-2 transcript has been identified by transcriptional profiling of rat kidneys as being regulated by dietary salt. We compared renal AQP-2 transcript expression in Sprague-Dawley and Dahl salt-sensitive (SS/Jr) rats using real-time RT-PCR. Expression of AQP-2 transcript is 5-fold less (P<0.01) in the Sprague-Dawley and 3-fold greater in Dahl SS/Jr rats (P<0.01) on high versus basal NaCl diets. The AQP-2 coded sequence was identical in Sprague-Dawley and Dahl SS/Jr rats. The present results provide evidence that: (1)AQP-2 plays a role in salt adaptation and (2) regulation of aquaporin transcript expression by salt is altered in the Dahl SS/Jr rat.
Roxas
Bryan
B
Department of Medicine, West Side Veterans Administration and University of Illinois-Chicago, 840 S. Wood Street, Chicago, IL 60612, USA.
Farjah
Mariam
M
Danziger
Robert S
RS
D000222:Adaptation, Physiological; D000284:Administration, Oral; D000818:Animals; D051399:Aquaporin 2; D051403:Aquaporin 6; D020346:Aquaporins / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008297:Male; D051381:Rats; D020303:Rats, Inbred Dahl; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology*; D013045:Species Specificity; D014158:Transcription, Genetic
C496025:Aqp2 protein, rat; D051399:Aquaporin 2; D051403:Aquaporin 6; D020346:Aquaporins; D012965:Sodium Chloride