title: Morphology and life cycle of Apatemon hypseleotris species novum from Australia including metacercariae viability and excystment.
abstract: Experimental infection of pigeon squabs and rats with encysted metacercariae from the Western Carp gudgeon (Hypseleotris klunzingeri) showed them to be infected with a new strigeid trematode, Apatemon hypseleotris. Growth and development of A. hypseleotris in pigeons were significantly higher than in rats. Eggs appeared in pigeon faeces within 7-14 days; miracidia hatched within 15-21 days and in the snail Lymnaea tomentosa released within 21 days. Cercariae experimentally encysted in the leeches Helobdella papillornata (86.7%) and Alboglossiphonia australiensis (73.3%). In fish, encystation occurred in the abdominal cavity (100%) and muscles (40%) of Hypseleotris klunzingeri, in the abdominal cavity (80%) and head (30%) of Gambusia affinis and in the abdominal cavity (62.5%) of Oncorhynchus mykiss but no encystation occurred in Moenkhausia pittieri. Freezing (-7 degrees C for 3-7 days or -21 degrees C for 8-12 hours), chilling (6 degrees C for 12 days), boiling (3 minutes) or salting for 3-5 days of encysted metacercariae did not significantly reduce infectivity. In vitro excystation of metacercariae was achieved using pepsin followed by trypsin and/or bile salts.
authors.affiliation: Benha University, Department of Parasitology, Faculty of Vet. Med., Tukh Moushtohour, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Negm-Eldin
authors.forename: R W
authors.initials: RW
authors.lastname: Davies
mesh_terms: D000818:Animals; D001315:Australia / Q000453:epidemiology; D010856:Columbidae; D005243:Feces / Q000469:parasitology; D005393:Fish Diseases / Q000453:epidemiology / Q000469:parasitology*; D007421:Intestine, Small / Q000469:parasitology; D007865:Leeches / Q000469:parasitology; D008018:Life Cycle Stages; D008195:Lymnaea / Q000469:parasitology; D010473:Perciformes / Q000469:parasitology*; D015995:Prevalence; D051381:Rats; D013045:Species Specificity; D014200:Trematoda / Q000033:anatomy & histology / Q000254:growth & development* / Q000502:physiology; D014201:Trematode Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*