Previously uncharacterized genes in the UV- and MMS-induced DNA damage response in yeast.
A competitive growth assay has been used to identify yeast genes involved in the repair of UV- or MMS-induced DNA damage. A collection of 2,827 yeast strains was analyzed in which each strain has a single ORF replaced with a cassette containing two unique sequence tags, allowing for its detection by hybridization to a high-density oligonucleotide array. The hybridization data identify a high percentage of the deletion strains present in the collection that were previously characterized as being sensitive to the DNA-damaging agents. The assay, and subsequent analysis, has been used to identify six genes not formerly known to be involved in the damage response, whose deletion renders the yeast sensitive to UV or MMS treatment. The recently identified genes include three uncharacterized ORFs, as well as genes that encode protein products implicated in ubiquitination, gene silencing, and transport across the mitochondrial membrane. Epistatsis analysis of four of the genes was performed to determine the DNA damage repair pathways in which the protein products function.
Hanway
Denise
D
Department of Chemistry and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Chin
Jodie K
JK
Xia
Gang
G
Oshiro
Guy
G
Winzeler
Elizabeth A
EA
Romesberg
Floyd E
FE
D004249:DNA Damage; D004271:DNA, Fungal / Q000187:drug effects* / Q000528:radiation effects*; D004843:Epistasis, Genetic; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D009153:Mutagens; D008741:Methyl Methanesulfonate