Immunoreactivity for myeloperoxidase (MPO) in the vestibule after the injection of bacterial lipopolysaccharide into the middle ear.
OBJECTIVE
In this study, the effect of endotoxin on the vestibule of the ear of guinea pigs was immunohistochemically examined.


METHODS
Bacterial lipopolysaccharide was injected into the middle ear transtympanically. After 48 h, the animals were sacrificed by intracardiac perfusion of fixative; then, the temporal bones were removed and processed for immunohistochemical staining with the anti-myeloperoxidase antibody.


RESULTS
Myeloperoxidase could be detected after 48 h in the sensory epithelium and the dark cell area.


CONCLUSIONS
It is reported that radical oxygen species, which are cytotoxic, are detected under inflammatory conditions. Our results suggest that myeloperoxidase and reactive oxygen species are involved in vestibular dysfunction under inflammation.
Watanabe
Ken-ichi
K
Department of Oto-Rhino-Laryngology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan. bxp0264@nifty.ne.jp
Jinnouchi
Ken
K
Yagi
Toshiaki
T
D000818:Animals; D004848:Epithelium / Q000473:pathology; D006168:Guinea Pigs; D018069:Hair Cells, Vestibular / Q000473:pathology; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000276:immunology*; D009195:Peroxidase / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014722:Vestibule, Labyrinth / Q000473:pathology*
D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D009195:Peroxidase