title: Changes of myocardial mitochondrial Ca(2+) transport and mechanism in the early stage after severe burns.
abstract: OBJECTIVE
  To investigate the change of myocardial mitochondrial Ca(2+) transport and its mechanism in the early stage after burns.
  
  
  METHODS
  Forty-eight Wistar rats were randomized into a normal control group (n=8) and a burns group (n=40). The rats of the burns group were given a 30%TBSA full-thickness. Myocardial mitochondria were isolated from normal and scalded rats which were sacrificed at the 1st, 3rd, 6th, 12th and 24th hour post-burn. Mitochondrial Ca(2+) transport velocity, membrane potential (MP), ATP content and cytosolic Ca(2+) concentration [Ca(2+)](c) were determined. The effects of exogenous ATP on mitochondrial Ca(2+) transport velocity were also investigated.
  
  
  RESULTS
  Mitochondrial Ca(2+) uptake velocity of the 1st hour post-burn was higher than that of the control, and Ca(2+) release velocity did not change significantly, but mitochondrial Ca(2+) transport velocity, MP and ATP content were all decreased at the 3rd, 6th, 12th and 24th hour post-burn. Mitochondrial Ca(2+) uptake velocity was positively correlated with MP after burn, and Ca(2+) release velocity with mitochondrial ATP content. [Ca(2+)](c) was increased at the 3rd, 6th, 12th and 24th hour post-burn. Exogenous ATP increased myocardial mitochondrial Ca(2+) uptake velocity of rats at the 3rd and 6th hour post-burn and Ca(2+) release velocity at the 3rd, 6th and 12th hour post-burn.
  
  
  CONCLUSIONS
  Increase of [Ca(2+)](c) led to reinforcement of mitochondrial Ca(2+) uptake at the beginning of the post-burns period. ATP depletion and MP collapse cause myocardial mitochondrial Ca(2+) transport disorder in the following stages.
authors.affiliation: Institute of Burns Research, Southwest Hospital, Third Military Medical University, 400038, Chongqing, China. lwy@mail.tmmu.com.cn
authors.forename: Liang
authors.initials: L
authors.lastname: Wan-YI
authors.forename: Tao
authors.initials: T
authors.lastname: Hui
authors.forename: Yang
authors.initials: Y
authors.lastname: Zong-Cheng
authors.forename: Huang
authors.initials: H
authors.lastname: Yue-Sheng
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002056:Burns / Q000378:metabolism*; D002118:Calcium / Q000032:analysis; D004195:Disease Models, Animal; D017136:Ion Transport / Q000502:physiology*; D008929:Mitochondria, Heart / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D015599:Trauma Severity Indices
chemical_list: D000255:Adenosine Triphosphate; D002118:Calcium