Hydrogen peroxide generation in caco-2 cell culture medium by addition of phenolic compounds: effect of ascorbic acid.
Phenolic compounds have recently attracted special attention due to their beneficial health effects; their intestinal absorption and bioavailability need, therefore, to be investigated and Caco-2 cell culture model appeared as a promising tool. We have shown herein that the addition of a grape seed extract (GSE) to Dulbecco's modified Eagle's medium (DMEM) used for Caco-2 cell culture leads to a substantial loss of catechin, epicatechin and B2 and B3 dimers from GSE in the medium after 24 h and to a production of hydrogen peroxide (H2O2). When 1420 microM ascorbic acid is added to the DMEM, such H2O2 production was prevented. This hydrogen peroxide generation substantially involves inorganic salts from the DMEM. We recommend that ascorbic acid be added to circumvent such a risk.
Roques
Sylvie Cambon
SC
Unité Nutrition, Laboratoire Génie Biologique et Sciences des Aliments, Université Montpellier II, France.
Landrault
Nicolas
N
Teissèdre
Pierre-Louis
PL
Laurent
Caroline
C
Besançon
Pierre
P
Rouane
Jean-Max
JM
Caporiccio
Bertrand
B
D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D018938:Caco-2 Cells / Q000187:drug effects* / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000378:metabolism; D003470:Culture Media; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism*; D027843:Vitis / Q000737:chemistry / Q000378:metabolism
D000975:Antioxidants; D003470:Culture Media; D002392:Catechin; D006861:Hydrogen Peroxide; C045651:epigallocatechin gallate; D001205:Ascorbic Acid