title: Performance of an IS6110-based PCR assay and the COBAS AMPLICOR MTB PCR system for detection of Mycobacterium tuberculosis complex DNA in human lymph node samples.
abstract: We compared the performance of two PCR assays, an IS6110-based in-house protocol and the COBAS AMPLICOR MTB PCR (COBAS MTB) system, for the detection of Mycobacterium tuberculosis complex in 43 human lymph node samples from 40 patients. For the in-house PCR and the COBAS MTB assays, respectively, sensitivities were 87.5% versus 45.5% (P < 0.05), specificities were 100.0% versus 91.3% (P > 0.05), and inhibition rates were 4.8% versus 19.5% (P < 0.05). For the COBAS MTB system, additional N-acetyl-L-cysteine-NaOH pretreatment of the samples changed neither the inhibition rate nor the sensitivity significantly.
authors.affiliation: Department of Medical Microbiology and Hospital Hygiene, University Hospital, D-18057 Rostock, Germany. dagmar.rimek@med.uni-rostock.de
authors.forename: Dagmar
authors.initials: D
authors.lastname: Rimek
authors.forename: Sachin
authors.initials: S
authors.lastname: Tyagi
authors.forename: Reinhard
authors.initials: R
authors.lastname: Kappe
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D004251:DNA Transposable Elements / Q000235:genetics*; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014388:Tuberculosis, Lymph Node / Q000382:microbiology*
chemical_list: D004251:DNA Transposable Elements