Mutagenicity and DNA-damaging activity caused by decomposed products of potassium sorbate reacting with ascorbic acid in the presence of Fe salt.
Although potassium sorbate (PS), ascorbic acid and ferric or ferrous salts (Fe-salts) are used widely in combination as food additives, the strong reactivity of PS and oxidative potency of ascorbic acid in the presence of Fe-salts might form toxic compounds in food during its deposit and distribution. In the present paper, the reaction mixture of PS, ascorbic acid and Fe-salts was evaluated for mutagenicity and DNA-damaging activity by means of the Ames test and rec-assay. Effective lethality was observed in the rec-assay. No mutagenicity was induced in either Salmonella typhimurium strains TA98 (with or without S-9 mix) or TA100 (with S-9 mix). In contrast, a dose-dependent mutagenic effect was obtained when applied to strain TA100 without S-9 mix. The mutagenic activity became stronger increasing with the reaction period. Furthermore, the reaction products obtained in a nitrogen atmosphere did not show any mutagenic and DNA-damaging activity. PS, ascorbic acid and Fe-salts were inactive when they were used separately. Omission of one component from the mixture of PS, ascorbic acid and Fe-salt turned the reaction system inactive. These results demonstrate that ascorbic acid and Fe-salt oxidized PS and the oxidative products caused mutagenicity and DNA-damaging activity.
Kitano
K
K
Osaka City Institute of Public Health and Environment Sciences, Osaka 543-0023, Japan.
Fukukawa
T
T
Ohtsuji
Y
Y
Masuda
T
T
Yamaguchi
H
H
D001205:Ascorbic Acid / Q000737:chemistry*; D004249:DNA Damage; D011756:Diphosphates / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry; D005520:Food Preservatives / Q000737:chemistry / Q000633:toxicity*; D007501:Iron / Q000737:chemistry*; D009152:Mutagenicity Tests; D012269:Ribosomal Proteins; D012486:Salmonella typhimurium / Q000235:genetics; D013011:Sorbic Acid / Q000737:chemistry / Q000633:toxicity*
D011756:Diphosphates; D005290:Ferric Compounds; D005296:Ferrous Compounds; D005520:Food Preservatives; D012269:Ribosomal Proteins; C042664:ribosomal protein BL16; C020748:ferrous sulfate; C025314:ferric citrate; D004492:Edetic Acid; D007501:Iron; C019179:Fe(III)-EDTA; D001205:Ascorbic Acid; C049051:ferric pyrophosphate; C011819:ferrous gluconate; D013011:Sorbic Acid