Palindromic unit-independent transposition of IS1397 in Yersinia pestis.
Palindromic units (PUs) are intergenic repeated sequences scattered over the chromosomes of Escherichia coli and several other enterobacteria. In the latter, IS1397, an E. coli insertion sequence specific to PUs, transposes into PUs with sequences close to the E. coli consensus. Reasons for this insertion specificity can relate to either a direct recognition of the target (by its sequence or its structure) by the transposase or an interaction between a specific host protein and the PU target DNA sequence. In this study, we show that for Yersinia pestis, a species deprived of PUs, IS1397 can transpose onto its chromosome, with transpositional hot spots. Our results are in favor of a direct recognition of target DNA by IS1397 transposase.
Wilde
Caroline
C
Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS URA 1444, Laboratoire des Yersinia, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
Bachellier
Sophie
S
Hofnung
Maurice
M
Carniel
Elisabeth
E
Clément
Jean-Marie
JM
D001483:Base Sequence; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015010:Yersinia pestis / Q000235:genetics*
D004251:DNA Transposable Elements