title: Isolation, purification, and biochemical characterization of a novel water soluble protein from Inca peanut (Plukenetia volubilis L.).
abstract: A water soluble storage albumin from Inca peanut (IPA) accounted for approximately 25% (w/w) of defatted seed flour weight, representing 31% of the total seed protein. IPA is a 3S storage protein composed of two glycosylated polypeptides, with estimated molecular weights (MW) of 32800 and 34800 Da, respectively. IPA has an estimated sugar content of 4.8% +/- 0.92% (n = 6). IPA is a basic protein (pI of approximately 9.4) and contains all of the essential amino acids in adequate amounts when compared to the FAO/WHO recommended pattern for a human adult. The tryptophan content of IPA is unusually high (44 mg/g of protein), whereas the phenylalanine content is low (9 mg/g of protein). IPA is a highly digestible protein in vitro.
authors.affiliation: Department of Nutrition, Food and Exercise Sciences, College of Human Sciences, Florida State University, Tallahassee, Florida 32306-1493, USA. ssathe@mailer.fsu.edu
authors.forename: Shridhar K
authors.initials: SK
authors.lastname: Sathe
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Hamaker
authors.forename: Kar Wai Clara
authors.initials: KW
authors.lastname: Sze-Tao
authors.forename: Mahesh
authors.initials: M
authors.lastname: Venkatachalam
mesh_terms: D000595:Amino Acid Sequence; D000601:Amino Acids, Essential / Q000032:analysis; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D006358:Hot Temperature; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010649:Phenylalanine / Q000032:analysis; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D011489:Protein Denaturation; D012639:Seeds / Q000737:chemistry*; D014364:Tryptophan / Q000032:analysis; D014867:Water
chemical_list: D000601:Amino Acids, Essential; D010940:Plant Proteins; D014867:Water; D010649:Phenylalanine; D014364:Tryptophan; D010450:Endopeptidases