Reverse Oxygen Effect on gamma-Irradiation Induced Single Strand Breaks of Plasmid DNA.
Oxygen effect on gamma-irradiation induced single strand breaks (SSB) of plasmid DNA was measured by the method of gel scanning. It was found that, in the presence of mannitol, the yield of SSB in N(2)-saturated DNA solution was larger than that in air-saturated solution so that the oxygen enhancement ratio (OER) of SSB became smaller than 1, which indicates the appearance of reverse oxygen effect of DNA SSB. At the same time, G(SSB) and its OER decreased with increasing concentration of mannitol.
Shao
Chun-Lin
CL
Institute of Plasma Physics, the Chinese Academy of Sciences, P P Box 1126, Hefei 230031, China. CLShao@ipncl.hfcas.ac.cn
Saito
Masahiro
M
Yu
Zeng-Liang
ZL