Alteration of E-cadherin and beta-catenin in mouse vestibular epithelia during induction of apoptosis.
The aim of this study was to examine if adhesion molecules had relation with degeneration and regeneration processes of mammalian vestibular epithelia. The distribution of E-cadherin and beta-catenin was immunohistochemically examined in normal and aminoglycoside-treated utricles of mice. E-cadherin and beta-catenin linearly expressed between epithelial cells in normal specimens. Aminoglycoside injury resulted in temporal alteration in distribution of these molecules with induction of apoptosis in hair cells. Degradation of both molecules was widely observed in vestibular epithelia, while some supporting cells exhibited accumulation of beta-catenin. After completion of induction of apoptosis, expression of these adhesion molecules was normal in distribution. These findings suggest that the E-cadherin-beta-catenin complex plays roles in degeneration and subsequent repair processes in vestibular epithelia affected by aminoglycosides.
Kim
Tae Soo
TS
Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, 606-8507 Kyoto, Japan.
Nakagawa
Takayuki
T
Endo
Tsuyoshi
T
Iguchi
Fukuichiro
F
Murai
Norihiko
N
Naito
Yasushi
Y
Ito
Juichi
J
D000617:Aminoglycosides / Q000009:adverse effects; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D015820:Cadherins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D004847:Epithelial Cells / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D012444:Saccule and Utricle / Q000737:chemistry / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015534:Trans-Activators / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D014722:Vestibule, Labyrinth / Q000737:chemistry / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051176:beta Catenin
D000617:Aminoglycosides; C495267:CTNNB1 protein, mouse; D015820:Cadherins; D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D051176:beta Catenin