Antimutagenic effect of amino acids on the mutagenicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
The antimutagenic activity of protein-constituting amino acids except histidine on the mutagenicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was investigated in vitro using Salmonella typhinurium TA-100 as an indicator bacterium (Ames test), and concentrations (IC50) of amino acids that inhibit 50% of the mutagenecity were measured. Cysteine was found to be most active and glycine, tryptophan, lysine, and arginine were strong antimutagenic amino acids. Other amino acids showed moderate or weak antimutagenic activities, depending on the amino acids. The results indicate that amino acids play a substantial role in chemoprevention of N-nitroso amine-induced mutagenicity.
Roy
Molay Kumar
MK
Department of Bioresource, Faculty of Agriculture, Ehime University, Matsuyama, Japan.
Kuwabara
Yuji
Y
Hara
Kazusi
K
Watanabe
Yasuo
Y
Tamai
Youichi
Y
D000596:Amino Acids / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D016296:Mutagenesis / Q000187:drug effects*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D000596:Amino Acids; D016587:Antimutagenic Agents; D009153:Mutagens; D008769:Methylnitronitrosoguanidine