Synthesis of ODNs containing 4-methylamino-1,8-naphthalimide as a fluorescence probe in DNA.
Synthesis and fluorescence properties of oligodeoxynucleotides containing 4-methylamino-1,8-naphthalimide (NI) have been described. NI was successfully incorporated into DNA without significant destabilization of DNA whilst retaining its high fluorescence quantum yield. The attachment site of the NI greatly affected its property as an energy acceptor in FRET analysis.
Kawai
Kiyohiko
K
The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka, Japan. kiyohiko@sanken.osaka-u.ac.jp
Kawabata
Kazuhiro
K
Tojo
Sachiko
S
Majima
Tetsuro
T
D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007094:Imides / Q000138:chemical synthesis*; D009281:Naphthalenes / Q000138:chemical synthesis*; D053644:Naphthalimides; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis
C475719:4-methylamino-1,8-naphthalimide; D005456:Fluorescent Dyes; D007094:Imides; D009281:Naphthalenes; D053644:Naphthalimides; D009838:Oligodeoxyribonucleotides; D004247:DNA