title: [Study of cathepsin D levels in invasive bladder cancer and its stroma. Correlation with tumor stage, cytological grade, lymph node metastasis and survival].
abstract: OBJECTIVE
  The aim of this study was to evaluate cathepsin D as a prognostic marker in invasive bladder cancer and to determine its relationship with stage, grade, lymph-node metastasis and survival too.
  
  
  METHODS
  An immunohistochemical staining of 32 radical cystectomy specimens suffering from transitional cell carcinoma was performed, using a monoclonal antibody anti-cathepsin D (Novocastra). We made a semicuantitative measurement of the cathepsin D expression in the tumor and in the peritumoral stroma in a 400 x microscopic high power field. Patient population was composed of 31 men and 1 woman with a mean age of 63.25 years. The mean follow up was 23.6 months. Stage was classified with the WHO 1997 classification. Grade was classified with the ISUP/WHO 1998 classification. For the statistical analysis the Chi-square test, Pearson's test R, the Kaplan Meier method and the log-rank test were used.
  
  
  RESULTS
  The pathological stages of the surgical specimens were as follows: pTo:3.1% (1), pT1:12.5% (4), pT2:15.6% (5), pT3:34.4% (11). (p < 0.001) A high cytologic grade was found in 81.25% of the tumors. There was a 43.8% progression rate and 40.6% mortality. There was no statistically significant relationship among Cathepsin's D levels in the stroma and lymph node metastases, stage, or grade (p = 0.473, p = 0.604, p = 0.2423). There was no statistically significant relationship among Cathepsin's D levels in the tumor and lymph node metastases, stage or grade (p = 0.496, p = 0.722 and p = 0.461). The cathepsin D levels, neither in the stroma nor in the tumor, showed no influence neither on the disease free intervals nor in the survival rates (p = 0.785; p = 0.355 and p = 0.614; p = 0.601 respectively).
  
  
  CONCLUSIONS
  Immunohistochemical Cathepsin D levels do not seem to play a role in the prognostic of transitional tumors of the urinary bladder.
authors.affiliation: Servicio de Urología, Hospital Dr. Peset, Valencia.
authors.forename: C
authors.initials: C
authors.lastname: Carrascosa Lloret
authors.forename: A
authors.initials: A
authors.lastname: Cremades Mira
authors.forename: M
authors.initials: M
authors.lastname: Sánchez Sanchis
authors.forename: V
authors.initials: V
authors.lastname: Rodrigo Guanter
authors.forename: J R
authors.initials: JR
authors.lastname: Beltrán Armada
authors.forename: A
authors.initials: A
authors.lastname: Sala Aznar
authors.forename: R
authors.initials: R
authors.lastname: Vacacela Córdova
authors.forename: B
authors.initials: B
authors.lastname: Coronel Sánchez
authors.forename: F
authors.initials: F
authors.lastname: Clar Blanch
authors.forename: M
authors.initials: M
authors.lastname: Tarin Planes
authors.forename: C
authors.initials: C
authors.lastname: Sanjuán de Laorden
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D002295:Carcinoma, Transitional Cell / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D002402:Cathepsin D / Q000032:analysis*; D016009:Chi-Square Distribution; D015653:Cystectomy; D003627:Data Interpretation, Statistical; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D013997:Time Factors; D001743:Urinary Bladder / Q000737:chemistry* / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D002402:Cathepsin D