title: Retinoblastoma-related gene RB2/p130 exons 19-22 are rarely mutated in glioblastomas.
abstract: The retinoblastoma gene family RB1, p107 and RB2/p130 cooperate to regulate cell cycle progression through the G1 phase of the cell cycle. Previous data demonstrated that RB2/p130 inhibits proliferation of the glioblastoma cell line T98G, which is resistant to the growth suppressive effects of both RB1 and p107, and that RB2/p130 gene overexpresion induces astrocyte differentiation. We screened by single-strand conformation polymorphism and sequence analysis the structure of exons 19 through 22 of the RB2/p130 gene, which encodes the B domain and C terminus, in a series of 42 glioblastomas (32 primary and 10 secondary). Sequence variations were identified in one tumor, suggesting that mutation inactivation of RB2/p130 is a rare event in glioblastoma.
authors.affiliation: Departamento C. Experimental, Hospital Universitario La Paz, Paseo de la Castellana 261, 28046 Madrid, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Gonzalez-Gomez
authors.forename: M Josefa
authors.initials: MJ
authors.lastname: Bello
authors.forename: Dolores
authors.initials: D
authors.lastname: Arjona
authors.forename: M Eva
authors.initials: ME
authors.lastname: Alonso
authors.forename: Jesus
authors.initials: J
authors.lastname: Lomas
authors.forename: Jose M
authors.initials: JM
authors.lastname: De Campos
authors.forename: Jesus
authors.initials: J
authors.lastname: Vaquero
authors.forename: Alberto
authors.initials: A
authors.lastname: Isla
authors.forename: Manuel
authors.initials: M
authors.lastname: Gutierrez
authors.forename: Juan A
authors.initials: JA
authors.lastname: Rey
mesh_terms: D001253:Astrocytes / Q000166:cytology; D001483:Base Sequence; D002453:Cell Cycle; D002454:Cell Differentiation; D002455:Cell Division; D004252:DNA Mutational Analysis; D005091:Exons; D016193:G1 Phase; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D010750:Phosphoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017434:Protein Structure, Tertiary; D011506:Proteins; D050717:Retinoblastoma-Like Protein p130; D014407:Tumor Cells, Cultured
chemical_list: D010750:Phosphoproteins; D011506:Proteins; C493837:RBL2 protein, human; D050717:Retinoblastoma-Like Protein p130