[A study of the effect of locus control region elements HS2 and HS3 on beta-globin gene expression].
HS2 and HS3 are important elements of human beta-globin locus control region (LCR). To study the effect of HS2, HS3, and HS2-HS3 on human beta-globin gene expression, a series of expression cassettes were constructed, in which the reporter gene encoding the enhanced green fluorescent protein (EGFP) was driven by the beta-globin promotor and under the control of HS2, HS3, and HS2-HS3. These constructs were transfected transiently into MEL and K562 cell lines mediated with liposome and their expression was measured with FACS as well as semi-quantitative RT-PCR method. The results showed that 3.2-kb HS2 could significantly enhance the activity of beta-globin promotor in MEL and K562 cells, while 3-kb HS3 could do only in MEL cells. There was no synergistic function in transient expression in the cassettes with the combination of HS2 and HS3 in MEL and K562 cells.
Jia
Chun-Ping
CP
Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai 200040, China.
Huang
Shu-Zhen
SZ
Zeng
Yi-Tao
YT
D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D019901:Locus Control Region
D005914:Globins