[Properties of endoribonuclease activity of proteasomes from K562 cells. III. The specific endonuclease activity of ribonucleoprotein particles (alpha-RNP) tightly bound to chromatin and containing 20S proteosomes].
The present work demonstrates the ability of 20S proteasome-containing, tightly bound to chromatin RNP-particles (alpha-RNP) to endonucleolyse specific messenger RNAs (in particular, human mRNA for p53 gene and mRNA for luciferase from Renilla sp.). The dependence of individual mRNA endonucleolysis by alpha-RNP particles on both the substrate and enzyme was found.
Mittenberg
A G
AG
Institute of Cytology RAS, St. Petersburg. agm@mail.cytspb.rssi.ru
Kulichkova
V A
VA
Gauze
L N
LN
Konstantinova
I M
IM
D002843:Chromatin / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000032:analysis / Q000378:metabolism*; D004722:Endoribonucleases / Q000378:metabolism*; D016158:Genes, p53; D006801:Humans; D020014:K562 Cells / Q000201:enzymology*; D008156:Luciferases / Q000235:genetics; D009097:Multienzyme Complexes / Q000032:analysis / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D012333:RNA, Messenger / Q000378:metabolism; D020736:Ribonucleoproteins, Small Cytoplasmic / Q000737:chemistry / Q000378:metabolism*
D002843:Chromatin; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D020736:Ribonucleoproteins, Small Cytoplasmic; D008156:Luciferases; D004722:Endoribonucleases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex