title: The serine proteinase thrombin promotes migration of human renal carcinoma cells by a PKA-dependent mechanism.
abstract: In this study, we demonstrated that thrombin activates protein kinase C (PKC), mitogen activated protein kinases (MAP kinases), transcription factor nuclear factor-kappa B (NF-kappa B), and cAMP-dependent protein kinase (PKA) in the human renal carcinoma cell line A-498. In addition, it enhanced the migratory capacity, but had no effect on the proliferation of A-498 cells. The effect of thrombin on migration could be blocked by the PKA inhibitor H-89 but was not influenced by inhibition of PKC, MAP kinases or NF-kappa B. We concluded, that thrombin acts as a regulator on human A-498 renal carcinoma cell migration including PKA.
authors.affiliation: Department of General and Visceral Surgery, Medical Faculty at the Friedrich Schiller University Jena, Bachstr. 18, 07740 Jena, Germany. roland.kaufmann@med.uni-jena.de
authors.forename: R
authors.initials: R
authors.lastname: Kaufmann
authors.forename: U
authors.initials: U
authors.lastname: Junker
authors.forename: K
authors.initials: K
authors.lastname: Junker
authors.forename: K
authors.initials: K
authors.lastname: Nuske
authors.forename: C
authors.initials: C
authors.lastname: Ranke
authors.forename: M
authors.initials: M
authors.lastname: Zieger
authors.forename: J
authors.initials: J
authors.lastname: Scheele
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism; D002633:Chemotaxis / Q000187:drug effects; D017868:Cyclic AMP-Dependent Protein Kinases / Q000502:physiology*; D004789:Enzyme Activation; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011493:Protein Kinase C / Q000502:physiology; D013917:Thrombin / Q000494:pharmacology*; D051996:Transcription Factor RelA; D014407:Tumor Cells, Cultured
chemical_list: D016328:NF-kappa B; D051996:Transcription Factor RelA; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D020928:Mitogen-Activated Protein Kinases; D013917:Thrombin