A Novel Reaction Media for Horseradish Peroxidase (HRP) Catalysis in Organic Solvents.
It is important to select favorable reaction media for use of enzymes in organic synthesis. In the presence of PEG with a certain molecular weight, toluene was able to dissolve horseradish peroxidase and the latter maintained a high activity. The HRP reaction conditions, such as ratio of PEG/toluene, water content, pH, and substrate concentration were investigated. It was found that the activity of HRP increased when PEG concentration was low and water content was high. In such a system, the enzyme functioned best at pH 7.0,the concentration of H(2)O(2) and guaiacol being 20 mmol/L and 0.1 mol/L respectively. Under the same condition, no activity was detected in the PEG / ethanol and PEG / dioxane systems. However, in PEG / toluene system, HRP maintained a higher activity and the activity was ten-fold more than that in toluene alone.
Jiang
Tai-Jiao
TJ
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. zhongyi@sunm.shcnc.ac.cn
Ji
Xin-Song
XS
Yuan
Zhong-Yi
ZY