Preferential expression of ADP-ribosylation factor gene in the chick embryonic gonads.
cDNA cloning from chick embryonic gonads subtracted from tissues of the brain, heart, liver, gizzard, mesonephros and skeletal muscle was performed to identify genes with expression unique to embryonic gonads. Several cDNA clones encoding characterized as well as many uncharacterized genes were obtained. ADP-ribosylation factor (ARF) of these identified genes was preferentially expressed in the chick embryonic ovary and testis as revealed by reverse transcription-polymerase chain reaction analysis. Expression of the ARF was evaluated through embryonic development, but no difference in the transcript (relative to glyceraldehyde-3-phosphate dehydrogenase transcript) was observed between the left and right ovaries, and between the ovary and testis. In addition, the ARF transcript was detected in the gonads on embryonic days 5 to 21. These findings indicate that the ARF is constantly, but preferentially expressed in the embryonic gonads during development.
Hattori
Masa-aki
MA
Division of Animal and Marine Resource Sciences, Graduate School of Agriculture, Kyushu University, Fukuoka, Japan. mhattori@agr.kyushu-u.ac.jp
Fujioka
Tsuyoshi
T
Kato
Yukio
Y
Fujihara
Noboru
N
D020727:ADP-Ribosylation Factors / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D002642:Chick Embryo; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D008297:Male; D010053:Ovary / Q000196:embryology / Q000378:metabolism*; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012727:Sex Characteristics; D013737:Testis / Q000196:embryology / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics
D018076:DNA, Complementary; D012313:RNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D020727:ADP-Ribosylation Factors