[Transgenic animals bioreactors].
The production of human recombinant proteins in milk of transgenic farm animals offers a safe, very cost-effective source of commercially important proteins that cannot be produced as efficiently in adequate quantities by other methods. This review has summarized the current status of gene selection, vector construct, transgenic methods, economics, and obvious potential in transgenic animals bioreactors. Recently, a more powerful approach was adopted in the transgenic animals founded on the application of nuclear transfer. As we will illustrate, this strategy presents a breakthrough in the overall efficiency of generating transgenic farm animals, product consistency, and time of product development. The successful adaptation of Cre-/lox P-mediated site-specific DNA recombination systems in farm animals will offer unprecedented possibilities for generating transgenic animals.
Gou
Ke-Mian
KM
National Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, China. goukm@mail.cau.edu.cn
An
Xiao-Rong
XR
Tian
Jian-Hui
JH
Chen
Yong-Fu
YF
D000818:Animals; D030801:Animals, Genetically Modified; D019149:Bioreactors; D001940:Breast / Q000378:metabolism*; D017690:Cell Transplantation; D015870:Gene Expression; D006801:Humans