[The effect of transposon P(GUS . p53.259H)on the frequency of tumor clones in Drosophila melanogaster wtsp2/+ heterozygotes].
We showed that transposon P(GUS.p53.259H), mapped to chromosome 3 and carrying a dominant mutation p53(259)H.GUS, has a positive effect on the frequency of spontaneous and carcinogen-induced tumor mosaic clones warts- in Drosopila melanogaster heterozygotes for the tumor suppressor gene warts located in the same chromosome. The transposon effect could be explained either by the arrest of apoptosis in the cells expressing mutant p53(259)H.GUS gene and containing carcinogen-induced pre-mutations, and/or by genetic instability introduced into chromosome 3 by the P(GUS.p53.259H) transposon itself. The effect of the P(GUS.p53.259H) appeared to be carcinogen-specific. It substantially increased the frequency of tumors induced by supermutagenic platinum complex, oxoplatin, and did not increase the frequencies of tumors induced by polycyclic aromatic hydrocarbons, benzo(alpha)pyrene and pyrene. In the spectrum of mutations induced by all carcinogens tested, somatic recombination events prevailed over somatic mutations. Hence, carcinogen-specificity of the P(GUS.p53.259H) effect cannot be explained by preferential induction of somatic mutations or somatic recombination by one of the carcinogens. Organ-specificity of the increased frequency of mosaic warts- clones induced by P(GUS.p53.259H) was established.
Sidorov
R A
RA
Institute of Carcinogenesis, N. N. Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478 Russia.
Belitskiĭ
G A
GA
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001580:Benzopyrenes / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity; D002945:Cisplatin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D016147:Genes, Tumor Suppressor; D006579:Heterozygote; D008297:Male; D009030:Mosaicism; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics*; D009928:Organ Specificity; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011721:Pyrenes / Q000633:toxicity; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
D001580:Benzopyrenes; D002273:Carcinogens; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D011721:Pyrenes; D016159:Tumor Suppressor Protein p53; C030984:pyrene; D011494:Protein Kinases; C093503:wts protein, Drosophila; D017346:Protein Serine-Threonine Kinases; D002945:Cisplatin; C043804:diamminedichlorodihydroxyplatinum IV