Identification of a criminal by DNA typing in a rape case in Rio de Janeiro, Brazil.
BACKGROUND
Human DNA identification is a powerful tool for paternity cases as well as for criminal investigation, in which biological evidence is typed after collection from crime scenes and for the identification of human remains.


OBJECTIVE
Identification of a criminal in a rape case with 4 suspects using STR and VNTR DNA analysis.


METHODS
Forensic DNA analysis.


METHODS
DNA Diagnostic Laboratory, Universidade Estadual do Rio de Janeiro, Brazil.


METHODS
Blood from 4 suspects and the victim, and skin from the fetus.


METHODS
Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).


RESULTS
Three of the suspects were excluded and one of them was identified as the biological father of the fetus after typing with CTT and FFv Multiplexes. Complementary DNA typing at 3 VNTR loci was also carried out.


CONCLUSIONS
After typing four suspects using 6 STR loci, one of them was identified as the biological father of the fetus. In order to significantly enhance the Combined Paternity Index (PI), complementary DNA typing in 3 VNTR loci was carried out. The included suspect was found to be the biological father with a PI of 412,860 (Probability of Paternity: 99.9997%).
Góes
Andréa Carla de Souza
AC
DNA Diagnostic Laboratory, Instituto de Biologia, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, Brazil. acgoes@uerj.br
Silva
Dayse Aparecida da
DA
Domingues
Cristiane Santana
CS
Sobrinho
João Marreiro
JM
Carvalho
Elizeu Fagundes de
EF
D000293:Adolescent; D001938:Brazil; D002650:Child Abuse, Sexual; D016172:DNA Fingerprinting; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011902:Rape; D012091:Repetitive Sequences, Nucleic Acid