Baculovirus-expressed muscovy duck reovirus sigmaC protein induces serum neutralizing antibodies and protection against challenge.
Recombinant baculoviruses with sigmaC- or sigmaB-encoding gene from muscovy duck reovirus (DRV) were constructed. Western-blot analysis showed that sigmaC was more immunoreactive than sigmaB. Vaccination of SPF ducks with two injections, 3 weeks apart, of emulsions containing sigmaC or sigmaC + sigmaB elicited DRV-specific neutralizing antibodies. Following challenge, vaccination partially--or even totally in some cases--prevented the appearance of clinical symptoms. Moreover, immunization reduced the severity of reovirus-induced tenosynovitis and prevented pericarditis development during the course of the assay. Thus, DRV sigmaC, alone or co-expressed with sigmaB, appeared as a good candidate for vaccination of ducks (96/100 mots).
Kuntz-Simon
Gaëlle
G
Avian and Rabbit Virology, Immunology and Parasitology Unit, Poultry and Swine Research Laboratory, French Agency for Food Safety (AFSSA), Zoopôle Les Croix, BP 53, 22440 Ploufragan, France.
Blanchard
Philippe
P
Cherbonnel
Martine
M
Jestin
André
A
Jestin
Véronique
V
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D004372:Ducks / Q000276:immunology*; D005260:Female; D005347:Fibroblasts / Q000821:virology; D005822:Genetic Vectors / Q000235:genetics*; D008297:Male; D009500:Neutralization Tests; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D030101:Orthoreovirus, Avian / Q000235:genetics / Q000276:immunology*; D010493:Pericarditis / Q000517:prevention & control / Q000662:veterinary / Q000821:virology; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D016601:RNA-Binding Proteins; D012088:Reoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms; D013717:Tenosynovitis / Q000517:prevention & control / Q000662:veterinary / Q000821:virology; D014611:Vaccination / Q000662:veterinary; D014614:Vaccines, Synthetic / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D014776:Virus Cultivation
D000914:Antibodies, Viral; D036022:Capsid Proteins; D016601:RNA-Binding Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; C031728:sigma 1 protein, reovirus; C053179:sigma protein 3, Reovirus