title: Two ATP synthases can be linked through subunits i in the inner mitochondrial membrane of Saccharomyces cerevisiae.
abstract: Cross-linking experiments showed that the supernumerary subunit i is close to the interface between two ATP synthases. These data were used to demonstrate the presence of ATP synthase dimers in the inner mitochondrial membrane of Saccharomyces cerevisiae. A cysteine residue was introduced into the inter-membrane space located C-terminal part of subunit i. Cross-linking experiments revealed a dimerization of subunit i. This cross-linking occurred only with the dimeric form of the enzyme after incubating intact mitochondria with a bis-maleimide reagent, thus indicating an inter-ATP synthase cross-linking, whereas the monomeric form of the enzyme exhibited only an intra-ATP synthase cross-linking with subunit 6, another component of the membranous domain of the ATP synthase.
authors.affiliation: Institut de Biochimie et Génétique Cellulaires du CNRS, Université Victor Segalen, Bordeaux 2, 1 rue Camille Saint-Saëns 33077 Bordeaux Cedex, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Paumard
authors.forename: Geneviève
authors.initials: G
authors.lastname: Arselin
authors.forename: Jacques
authors.initials: J
authors.lastname: Vaillier
authors.forename: Stéphane
authors.initials: S
authors.lastname: Chaignepain
authors.forename: Katell
authors.initials: K
authors.lastname: Bathany
authors.forename: Jean Marie
authors.initials: JM
authors.lastname: Schmitter
authors.forename: Daniel
authors.initials: D
authors.lastname: Brèthes
authors.forename: Jean
authors.initials: J
authors.lastname: Velours
mesh_terms: D000595:Amino Acid Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry*; D019281:Dimerization; D007425:Intracellular Membranes / Q000201:enzymology*; D008301:Maleimides / Q000737:chemistry; D008928:Mitochondria / Q000201:enzymology*; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry*; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D008301:Maleimides; D029701:Saccharomyces cerevisiae Proteins; C011328:N,N'-4-phenylenedimaleimide; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; C023860:N,N'-2-phenylenedimaleimide