Detection of enteroaggregative Escherichia coli with formalin-preserved HEp-2 cells.
Formalin-stored HEp-2 cells were used to assay Escherichia coli for adherence. Cells refrigerated in formalin for up to 28 days and used in a wet assay format demonstrated an assay sensitivity ranging from 94 to 98% to detect enteroaggregative E. coli (EAEC). HEp-2 cells first fixed and stored with formalin and then stored dry in ambient conditions for 6 weeks demonstrated an assay sensitivity of 92% to detect EAEC. Using formalin-fixed HEp-2 cells will improve the efficiency of EAEC identification.
Miqdady
Mohamad S
MS
Baylor College of Medicine, Houston, Texas, USA.
Jiang
Zhi-Dong
ZD
Nataro
James P
JP
DuPont
Herbert L
HL
D001422:Bacterial Adhesion; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000502:physiology; D004927:Escherichia coli Infections / Q000382:microbiology; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D014021:Tissue Preservation / Q000379:methods*
D005404:Fixatives; D005557:Formaldehyde