title: Sporanaerobacter acetigenes gen. nov., sp. nov., a novel acetogenic, facultatively sulfur-reducing bacterium.
abstract: A strictly anaerobic, moderately thermophilic, sporulating rod, designated strain Lup 33T, was isolated from an upflow anaerobic sludge blanket (UASB) reactor in Mexico. Strain Lup 33T possessed a few laterally inserted flagella, had a DNA G+C content of 32.2 mol % and grew optimally at pH 7.4 and 40 degrees C. Growth was observed at temperatures of up to 50 degrees C and was inhibited in the presence of 5% NaCl. Strain Lup 33T is heterotrophic and utilized some sugars, peptides and various single amino acids. Gelatin and casein were not used as energy sources. It performed the Stickland reaction and reduced elemental sulfur to sulfide. Acetate was the only fatty acid detected from glucose fermentation, whereas acetate together with isobutyrate and isovalerate were found as end products from peptone fermentation. Phylogenetically, strain Lup 33T branched with members of cluster XII of the order Clostridiales, with Clostridium hastiforme as the closest relative (similarity of 93%). On the basis of the phenotypic, genotypic and phylogenetic characteristics of the isolate, it is proposed as a novel species of a new genus, Sporanaerobacter acetigenes gen. nov., sp. nov. The type strain is strain Lup 33T (= DSM 13106T = CIP 106730T).
authors.affiliation: IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, Marseille, France.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Hernandez-Eugenio
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cayol
authors.forename: Bharat K C
authors.initials: BK
authors.lastname: Patel
authors.forename: Pierre
authors.initials: P
authors.lastname: Thomas
authors.forename: Hervé
authors.initials: H
authors.lastname: Macarie
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Garcia
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000693:Anaerobiosis; D001482:Base Composition; D019149:Bioreactors; D004275:DNA, Ribosomal; D005838:Genotype; D008800:Mexico; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013171:Spores, Bacterial / Q000502:physiology; D016999:Sulfur-Reducing Bacteria / Q000145:classification* / Q000235:genetics / Q000502:physiology
chemical_list: D000085:Acetates; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012722:Sewage