title: CD5- mantle cell lymphoma.
abstract: Mantle cell lymphoma (MCL) typically expresses B-cell antigens and CD5 and overexpresses bcl-1 protein. However, unusual cases of bcl-1+ and CD5-MCL have been observed, posing a practical challenge for correct diagnosis and management. We identified 25 cases (48 samples) of bcl-1+ and CD5- lymphoma. CD5 expression was assessed by flow cytometric analysis alone (1 case), immunohistochemical analysis alone (17 cases), or dual flow cytometric/immunohistochemical methods (7 cases). The morphologic features were consistent with MCL with centrocytic cytomorphology in 20 cases and blastic variant in 5 cases. The t(11;14) was confirmed in 8 of 11 cases by fluorescence in situ hybridization of paraffin-embedded tissue. Cytogenetic analysis revealed the t(11;14) within a complex karyotype in 2 additional cases. These data show that MCL may lack CD5 expression. Evaluation of bcl-1 expression by immunohistochemical analysis or molecular genetics may be indicated if MCL is suspected clinically or morphologically despite a lack of CD5 expression.
authors.affiliation: IMPATH, New York, NY 10019, USA.
authors.forename: Zach
authors.initials: Z
authors.lastname: Liu
authors.forename: Henry Y
authors.initials: HY
authors.lastname: Dong
authors.forename: Wojciech
authors.initials: W
authors.lastname: Gorczyca
authors.forename: Patricia
authors.initials: P
authors.lastname: Tsang
authors.forename: Patti
authors.initials: P
authors.lastname: Cohen
authors.forename: Christine F
authors.initials: CF
authors.lastname: Stephenson
authors.forename: Carol S
authors.initials: CS
authors.lastname: Berger
authors.forename: C Daniel
authors.initials: CD
authors.lastname: Wu
authors.forename: James
authors.initials: J
authors.lastname: Weisberger
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D018956:CD5 Antigens / Q000032:analysis* / Q000235:genetics; D002880:Chromosomes, Human, Pair 11; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D020522:Lymphoma, Mantle-Cell / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged
chemical_list: D018956:CD5 Antigens