title: Genotype of ethanol metabolizing enzyme genes by oligonucleotide microarray in alcoholic liver disease in Chinese people.
abstract: OBJECTIVE
  To explore the relationship between genetic polymorphisms of the ethanol metabolizing enzymes and the occurrence of alcoholic liver disease (ALD).
  
  
  METHODS
  Sixty-five healthy male controls and 165 alcoholisms (including 122 ALD patients and 43 male alcohol abusers without liver complications defined as alcohol-dependent) were analyzed by polymerase chain reaction and hybridized with oligonucleotide microarray to detect the polymorphisms of the ethanol metabolizing enzymes genes.
  
  
  RESULTS
  The frequencies of alcohol dehydrogenase gene 2 * 1 ( ADH2 * 1 ) allele were shown as 37.69%, 46.51% and 59.02% in control, alcohol-dependent and ALD groups respectively; while those of ADH2 * 2 allele were shown as 62.31 %, 53.49% and 40.98% respectively. No ADH2 * 3 was detected in any of the subjects. The frequency of ADH2 * 1 was significantly higher in alcoholisms (ALD group and alcohol-dependent group) than in healthy controls ( P < 0. 01), and significantly higher in ALD group than in alcohol-dependent group ( P < 0.05) . The frequency of ADH3 * 2 was significantly higher in alcohol-dependents than in healthy controls ( P < 0.05) . The frequencies of ALDH2 * 2 allele mutation were significantly lower in alcoholisms than that in the healthy controls, and the deference between ALD group and alcohol-dependent group was significant. No homozygotes for the mutant ALDH2 * 2 allele were found in either alcoholic groups.
  
  
  CONCLUSIONS
  Polymorphic ADH2, ADH3 and ALDH2 genes can affect the propensity for alcohol drinking in Chinese. The alleles of ADH2 * 2, ADH3 * 1 and ALDH2 * 2 are most likely to play a protective role against excessive consumption. ADH2 * 2 and ALDH2 X 2 may contribute to susceptibility for ALD.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Medical School, Zhejiang University, Hangzhou 310003, China.
authors.forename: Chaohui
authors.initials: C
authors.lastname: Yu
authors.forename: Youming
authors.initials: Y
authors.lastname: Li
authors.forename: Weixing
authors.initials: W
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Yue
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008108:Liver Diseases, Alcoholic / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011110:Polymorphism, Genetic
chemical_list: D007527:Isoenzymes; D000426:Alcohol Dehydrogenase; D000445:Aldehyde Oxidoreductases; C018699:aldehyde dehydrogenase (NAD(P)+)