Rolling properties of rGPIbalpha-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions.
The recombinant fragment of the platelet membrane glycoprotein, rGPIbalpha, was conjugated to phospholipid vesicles with the average diameter of ca. 1 microm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIbalpha-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIbalpha-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.
Takeoka
Shinji
S
Artificial Blood Project, Advanced Research Institute for Science and Engineering, Waseda University, Tokyo 169-8555, Japan.
Teramura
Yuji
Y
Okamura
Yosuke
Y
Tsuchida
Eishun
E
Handa
Makoto
M
Ikeda
Yasuo
Y
D001792:Blood Platelets / Q000502:physiology; D002465:Cell Movement; D005454:Fluorescence Polarization; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry; D008954:Models, Biological; D009038:Motion; D010743:Phospholipids / Q000737:chemistry*; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013314:Stress, Mechanical; D013696:Temperature; D014841:von Willebrand Factor / Q000378:metabolism*
D008081:Liposomes; D010743:Phospholipids; D019038:Platelet Glycoprotein GPIb-IX Complex; D011994:Recombinant Proteins; D014841:von Willebrand Factor