Characterization of four monoclonal antibodies to recombinant human tartrate-resistant acid phosphatase.
In this study we produced a recombinant human Tartrate-resistant acid phosphatase (TRAP) enzyme from baculovirus-infected insect cells, generated four monoclonal antibodies (MAbs) 15A4, 13B9, 1C6 and 3G7, to the enzyme, and characterized these antibodies. In the human serum and lung specimen, all four antibodies appeared to have a high specificity for native TRAP enzyme in western blot analysis, immunohistochemical analysis and enzyme immunoassay. These antibodies may react with respective conformational determinants, therefore, they may be useful for detection of active TRAP. Only one of the antibodies, 15A4 also reacted with a denatured epitope, therefore, it is suitable for western blot analysis, enzyme immunoassay and for immunohistochemistry in the rat. Taken together, having characterized properties of four monoclonal antibodies against recombinant human TRAP enzyme may be useful for development of TRAP specific immunoassays in pathology and hematology of the bone. They will certainly be of use for the study of biosynthesis, regulation and function of the TRAP enzyme.
Miyazaki
Takashi
T
Department of Biochemistry, Saitama Medical School, 38 Morohongo, Moroyama, Iruma-gun, Saitama 350-0495, Japan. miyasan@ns2.saitama-med.ac.jp
Matsunaga
Toshiyuki
T
Miyazaki
Shuichi
S
Hokari
Shigeru
S
Komoda
Tsugikazu
T
D000135:Acid Phosphatase / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001842:Bone and Bones / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D007527:Isoenzymes / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D009928:Organ Specificity / Q000276:immunology; D051381:Rats; D011994:Recombinant Proteins / Q000276:immunology*; D000071681:Tartrate-Resistant Acid Phosphatase
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D011994:Recombinant Proteins; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase