Changes in ceramide levels upon catechins-induced apoptosis in LoVo cells.
It has been demonstrated that there is difference in the induction of apoptosis in LoVo cells by (-)-epigallocatechin-3-gallate (EGCG), (-)-epigallocatechin (EGC), and (-)-epicatechin (EC). In this study, we explored changes in ceramide levels upon the three catechins-induced apoptosis in LoVo cells. Addition of C2- and C6-ceramide to LoVo cells mimicked EGCG or EGC in leading to apoptotic death. Further measurement of intracellular ceramide content showed that the treatment of LoVo cells with EGCG or EGC resulted in a rapidly transient increase in ceramide content, and then back gradually to base line level, whereas the action of EC was just opposite to that of EGCG or EGC. These results suggest there is difference in the generation of intracellular ceramide by the three catechins and ceramide may take part in the regulation of EGCG- or EGC-induced apoptosis in LoVo cells.
Tan
Xiaohua
X
Institute for Hepatic Diseases, Beijing Army General Hospital, People's Republic of China. txhua@btamail.net.cn
Zhang
Yali
Y
Jiang
Bo
B
Zhou
Dianyuan
D
D017209:Apoptosis / Q000187:drug effects*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002518:Ceramides / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D019852:Diacylglycerol Kinase / Q000032:analysis; D006801:Humans; D007700:Kinetics; D014407:Tumor Cells, Cultured
D002518:Ceramides; D002392:Catechin; C045651:epigallocatechin gallate; D019852:Diacylglycerol Kinase; C057580:gallocatechol