Oligodendrocytes from neural stem cells express alpha-synuclein: increased numbers from presenilin 1 deficient mice.
alpha-Synuclein normally a synaptic vesicle-associated cytoplasmic protein is the major component of filamentous inclusions of neurons in Parkinson's disease and dementia with Lewy bodies. It is also the major component of glial inclusions of multiple system atrophy. In characterizing cells derived from embryonic neural stem cells we found all oligodendrocytes had strong cytoplasmic expression of alpha-synuclein. Comparison of cells from presenilin 1 (PS1)-deficient mice with wild type revealed a 7-fold increase in oligodendrocytes. Western blotting analysis indicated the cells contained alpha-synuclein monomers and SDS-stable dimers and trimers. This cell system of oligodendroglial alpha-synuclein expression is a useful system to study alpha-synuclein metabolism in the cell type affected in multiple system atrophy. Increased oligodendroglial cell numbers from PS1-deficient cells provides further evidence for a role of PS1-dependent Notch signalling in cell fate decisions.
Culvenor
Janetta G
JG
Department of Pathology, The University of Melbourne and Mental Health Research Institute of Victoria, Parkville, Victoria 3010, Australia.
Rietze
Rodney L
RL
Bartlett
Perry F
PF
Masters
Colin L
CL
Li
Qiao-Xin
QX
D000818:Animals; D015153:Blotting, Western; D002452:Cell Count; D002454:Cell Differentiation; D002478:Cells, Cultured; D015870:Gene Expression; D002479:Inclusion Bodies / Q000737:chemistry; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains; D019578:Multiple System Atrophy / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis*; D009836:Oligodendroglia / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D053764:Presenilin-1; D013234:Stem Cells / Q000166:cytology*; D051843:Synucleins; D051844:alpha-Synuclein
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D053764:Presenilin-1; C497605:Snca protein, mouse; D051843:Synucleins; D051844:alpha-Synuclein