Isolation and characterization of the Spindly homologue from tomato.
The SPINDLY (SPY) gene is a crucial component of the gibberellin signal transduction pathway in Arabidopsis thaliana (L.) Heynh. In this study, the cloning of the SPINDLY-orthologous gene (LeSpy) from tomato (Lycopersicon esculentum Mill.) and its characterization are reported. SPY and LeSpy show high sequence similarity along their entire lengths, which is reflected in the conservation of exon-intron structure and of all sequence motives previously identified. To analyse the relationship between the Arabidopsis spindly and the tomato procera mutant, which show similar phenotypes, the LeSpy gene was characterized in wild-type and procera tomato plants. These analyses as well as mapping of LeSpy revealed that LeSpy and Procera are different genes.
Greb
Thomas
T
Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany.
Schmitz
Gregor
G
Theres
Klaus
K
D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D012097:Repressor Proteins; C101465:SPY protein, Arabidopsis; D004247:DNA