title: Cloning of Schistosoma japonicum Chinese Strain TPI Gene and Characterization of Its Expression Product in Escherichia coli.
abstract: Triose-phosphate isomerase is an important candidate for schistosoma antigens. An 800 bp DNA fragment was amplified by RT-PCR from adult Schistosoma japonicum mRNA. Sequence analysis revealed that this fragment contained S. japonicum (Chinese strain) triose-phosphate isomerase gene. Then this gene was cloned into the expression vector pGEX-4T and subsequently expressed in E. coli. The recombinant GST-fusion protein was purified by glutathione agarose affinity chromatography. Its molecular weight was determined to be 54 kD. The yield of expression was around 30 mg/L E. coli culture. Western blotting showed that the recombinant protein had good antigenicity which could be helpful for the making of anti-S. japonicum multi-valent recombinant vaccine.
authors.affiliation: Shanghai Institute of Animal parasitology, Chinese Academy of Agricultural Sciences, Shanghai 200232, China. caassp@public.sta.net.cn
authors.forename: Xue-Zhong
authors.initials: XZ
authors.lastname: Cai
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Lin
authors.forename: Guan-Zhen
authors.initials: GZ
authors.lastname: Yang
authors.forename: Fu-Hui
authors.initials: FH
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: You-Min
authors.initials: YM
authors.lastname: Cai
authors.forename: Xiang-Fu
authors.initials: XF
authors.lastname: Wu