Experimental study on He-Ne laser irradiation to inhibit scar fibroblast growth in culture.
OBJECTIVE
To explore the inhibitory effect of He-Ne laser irradiation on fibroblast growth of hypertrophic scars in culture.


METHODS
He-Ne laser with wavelength of 632.8 nm, power density of 50 mW/cm(2) and doses of 3 J/cm(2), 30 J/cm(2), 90 J/cm(2) and 180 J/cm(2) was used to irradiate human scar fibroblasts in culture 1, 3 and 5 times respectively, and then the cell count and cell cycle analysis were done.


RESULTS
Repeated irradiation with He-Ne laser at dose of 180 J/cm(2) three and five times led to an evident decrease in total cell number compared with that of the control group and there was a significant difference (P<0.05). The cell cycle analysis showed after three and five times of irradiation with 180 J/cm(2) He-Ne laser the cell number in S-phase decreased from 51% to 20% and 14% respectively, the cell number in G(0)/G(1) phase increased from 28% to 55% and 60% respectively, and the cell percentage in Sub-G1 phase was 6.7% and 9.8% respectively.


CONCLUSIONS
Repeated irradiation with 180 J/cm(2) He-Ne laser can inhibit scar fibroblasts growth in culture. It may be that He-Ne laser irradiation causes cell stagnation in G(0)/G(1) phase and apoptosis.
Shu
Bin
B
Department of Rehabilitation Medicine, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Wu
Zongyao
Z
Hao
Linlin
L
Zeng
Dengfen
D
Feng
Guangrui
G
Lin
Yonghui
Y
D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured; D002921:Cicatrix / Q000473:pathology*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects*; D006371:Helium; D006801:Humans; D007834:Lasers; D008297:Male; D009356:Neon
D006371:Helium; D009356:Neon