Ligand-dependent activation of breathless FGF receptor gene in Drosophila developing trachea.
Spatially and temporally regulated activity of Branchless/Breathless signaling is essential for trachea development in Drosophila. Early ubiquitous breathless (btl) expression is controlled by binding of Trachealess/Tango heterodimers to the btl minimum enhancer. Branchless/Breathless signaling includes a Sprouty-dependent negative feedback loop. We show that late btl expression is a target of Branchless/Breathless signaling and hence, Branchless/Breathless signaling contains a positive feedback loop, which may guarantee a continuous supply of fresh receptors to membranes of growing tracheal branch cells. Branchless/Breathless signaling activates MAP-kinase, which in turn, activates late btl expression and destabilizes Anterior-open, a repressor for late btl expression. Biochemical and genetic analysis indicated that the minimum btl enhancer includes binding sites of Anterior-open.
Ohshiro
Tomokazu
T
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyou-ku, Tokyo 113-0033, Japan.
Emori
Yasufumi
Y
Saigo
Kaoru
K
D000818:Animals; D001483:Base Sequence; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D008024:Ligands; D020935:MAP Kinase Signaling System; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases; D012313:RNA / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D015398:Signal Transduction; D014132:Trachea / Q000196:embryology*; D014158:Transcription, Genetic
C076188:AOP protein, Drosophila; D029721:Drosophila Proteins; D005136:Eye Proteins; D008024:Ligands; D017468:Receptors, Fibroblast Growth Factor; D012097:Repressor Proteins; D012313:RNA; C102301:BTL protein, Drosophila; D011505:Protein-Tyrosine Kinases