Tracing of projection neurons from the cervical dorsal horn to the medulla with the anterograde tracer biotinylated dextran amine.
In addition to the well-defined role of dorsal horn neurons in pain transmission, neurons in the superficial laminae also provide a rich source of synaptic input to cardiovascular and respiratory centers in the medullary reticular formation. In this study, ascending projection neurons from the superficial laminae of the cervical enlargement were studied in the rat using the anterograde tracer biotinylated dextran amine (BDA). Ipsilateral microinjection of BDA into the cervical spinal cord (C6-C8) resulted in extensive labeling of dorsal horn neurons in laminae I-V. Axons and terminal processes of cervical dorsal horn cells projecting to the medulla were present in the cuneate nucleus (Cu), the nucleus of the solitary tract (NTS), the lateral reticular nucleus, (LRt) as well as the caudal and rostral ventrolateral medulla (VLM). The highest density of BDA labeling was found ipsilaterally in the Cu, LRt, caudal and rostral VLM, while a moderate density of labeling was present in the NTS caudal to the area postrema (AP). Moderate-to-weak labeling was also found in the LRt, the caudal and rostral VLM contralateral to the BDA injection. These results support the existence of a spinomedullary pathway that transmits noxious and innocuous Adelta and C fiber-mediated sensory signals to the medulla. Neurons in this ascending spinal pathway likely participate in the patterning of autonomic responses evoked by pain or during exercise.
Potts
Jeffrey T
JT
Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA. jtpotts@med.wayne.edu
Lee
Sandra M
SM
Anguelov
Petio I
PI
D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D001710:Biotin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D003911:Dextrans / Q000008:administration & dosage; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D007278:Injections, Spinal; D008526:Medulla Oblongata / Q000502:physiology* / Q000648:ultrastructure; D009475:Neurons, Afferent / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D013116:Spinal Cord / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*
D003911:Dextrans; D005456:Fluorescent Dyes; C076397:biotinylated dextran amine; D001710:Biotin