Modelling of cell proliferation: nuclear versus membrane labelling.
Cell proliferation is a fundamental process involved in growth, development and oncogenesis. Monitoring and quantification of proliferation are essential to analyse the behaviour of cells drug-treated or not. Flow cytometry assessment of cell proliferation requires mathematical models to extract information of interest from fluorescence distributions. Various methods are available for cell cycle analysis, including estimation of cell phase durations and doubling time. In this context, we compare widely used flow cytometric methods based on nuclear labelling (using BrdUrd incorporation in combination with DNA content) to membrane labelling (using intercalating dyes PKH).
Barbier
Magali
M
Laboratoire de dynamique cellulaire, Ecole pratique des hautes études, CNRS UMR 5525, institut Albert-Bonniot, 38706 La Tronche, France. Magali.Barbier@ujf-grenoble.fr
Laurier
Jean-Fabien
JF
Seigneurin
Daniel
D
Ronot
Xavier
X
Boutonnat
Jean
J
D000818:Animals; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D008433:Mathematics; D008954:Models, Biological
D004247:DNA; D001973:Bromodeoxyuridine