Aldose reductase is an obligatory mediator of the late phase of ischemic preconditioning.
Aldose reductase (AR), a member of the aldo-keto reductase superfamily, has been shown to metabolize toxic aldehydes generated by lipid peroxidation, suggesting that it may serve as an antioxidant defense. To investigate its role in the late phase of ischemic preconditioning (PC), conscious rabbits underwent 6 cycles of 4-minute coronary occlusion/4-minute reperfusion. Twenty-four hours later, there was a marked increase in AR protein and activity and in the myocardial content of sorbitol, a unique product of AR catalysis. Pretreatment with N(omega)-nitro-L-arginine, a nitric oxide synthase inhibitor, or chelerythrine, a protein kinase C inhibitor (both given at doses that block late PC in this model), prevented the increase in AR protein 24 hours later, demonstrating that ischemic PC upregulates AR via nitric oxide- and protein kinase C-dependent signaling pathways. The AR-selective inhibitors tolrestat and sorbinil prevented AR-mediated accumulation of sorbitol and abrogated the infarct-sparing effect of late PC, demonstrating that enhanced AR activity is necessary for this cardioprotective phenomenon to occur. Inhibition of AR did not affect infarct size or the myocardial accumulation of the lipid peroxidation product 4-hydroxy trans-2-nonenal (HNE) in nonpreconditioned rabbits. The accumulation of HNE was inhibited by late PC, and this effect was abrogated by sorbinil. Taken together, these results establish AR as an essential mediator of late PC. Furthermore, the data suggest that myocardial ischemia/reperfusion injury is due in part to the generation of lipid peroxidation products and that late PC diminishes this source of injury by upregulating AR.
Shinmura
Ken
K
Experimental Research Laboratory, Division of Cardiology, University of Louisville and Jewish Hospital Heart and Lung Institute, Louisville, Ky, USA.
Bolli
Roberto
R
Liu
Si-Qi
SQ
Tang
Xian-Liang
XL
Kodani
Eitaro
E
Xuan
Yu-ting
YT
Srivastava
Sanjay
S
Bhatnagar
Aruni
A
D000449:Aldehyde Reductase / Q000502:physiology*; D000447:Aldehydes / Q000032:analysis; D000818:Animals; D002316:Cardiotonic Agents / Q000378:metabolism; D006439:Hemodynamics; D019157:Ischemic Preconditioning, Myocardial; D007700:Kinetics; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism; D008297:Male; D009203:Myocardial Infarction / Q000201:enzymology / Q000503:physiopathology / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control; D009206:Myocardium / Q000737:chemistry / Q000201:enzymology; D011817:Rabbits; D013012:Sorbitol / Q000032:analysis
D000447:Aldehydes; D002316:Cardiotonic Agents; D013012:Sorbitol; D007064:L-Iditol 2-Dehydrogenase; D000449:Aldehyde Reductase; C027576:4-hydroxy-2-nonenal