Ethanol decreases basal insulin secretion from HIT-T15 cells.
Various epidemiological studies suggest that alcohol intake is one of the risk factors leading to type II or non-insulin-dependent diabetes mellitus (NIDDM), but the effect of alcohol on beta-cell function remains unexplored. To study the mechanism of the diabetogenic action of ethanol, we investigated the effect of ethanol on beta-cell functions using a single clonal beta-cell line, HIT-T15 cells. When HIT cells were treated with ethanol, the metabolic activity judged by MTT assay was inhibited in dose- and time dependent manners, but cytotoxicity was not observed. Ethanol also inhibited basal insulin secretion by 30% compared to the untreated control. However, glucose-stimulated insulin secretion was not impaired by ethanol although the basal insulin secretion was inhibited. These results imply that ethanol exert beta-cells to overwork in order to compensate inhibition of the basal secretion. This finding may at least in part explain the diabetogenic action of ethanol.
Shin
Jun-Seop
JS
Graduate School of Biotechnology, Korea University, Seoul.
Lee
Jae-Jeong
JJ
Yang
Jae-Won
JW
Kim
Chan-Wha
CW
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003864:Depression, Chemical; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles
D007328:Insulin; D013778:Tetrazolium Salts; D013844:Thiazoles; D000431:Ethanol; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue