[Immobilization of immunoglobulins using lectins with an immune sensor method based on surface plasmon resonance].
The effect of different lectins upon the response of immune sensor based on surface plasmon resonance (SPR) was investigated. Lectins affinity to carbohydrates of the IgG can be used to increase the density and orientation of IgG molecules at their immobilisation on the sensor surface. Conditions were elaborated for enhancement of immune sensor response in comparison with that one for bare or preliminary treated with dodecanthiol thin gold sensor surface. It was shown that human IgG revealed maximal affinity to wheat germ lectin and mouse monoclonal antibodies and rabbit IgG--to helix pomatia lectin. Pig antibodies, similar to human IgG, showed the greatest affinity to wheat germ lectin.
Pyrohova
L V
LV
Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv.
Starodub
M F
MF
D000818:Animals; D015374:Biosensing Techniques; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D020349:Surface Plasmon Resonance
D007136:Immunoglobulins; D037102:Lectins