title: Expression in BHK Cells of Two Human TNF Receptors and Their Interaction with hTNF-alpha.
abstract: Two human tumor necrosis factor receptor genes were cloned into eukaryotic expression vectors pcDNA3, pDR2 and pXJ-41, respectively. Those vectors were transfected into BHK-21 cells and the expression of hTNFRs was identified and demonstrated by binding of (125)I hTNF-alpha and Scatchard analysis. The expression of two hTNFRs in BHK cells at the RNA transcriptional and the protein translation levels were also demonstrated by RT-PCR and indirect ELISA. However, hTNF-alpha did not appear cytotoxic to these cells. The competitive binding activity of mutant hTNF-alpha-R2K and wild type hTNF-alpha with these two receptors, respectively, were assayed. These results laid basis for further studies on structure-function relationships of these receptors as well as the hTNF-alpha.
authors.affiliation: Shanghai Research Center of Biotechnology, the Chinese Academy of Sciences, Shanghai 200233, China. cqchen@server.shcnc.ac.cn
authors.forename: Jia
authors.initials: J
authors.lastname: Fang
authors.forename: De-Bao
authors.initials: DB
authors.lastname: Wang
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Chen