Cytotoxicity of the venom of Pelagia noctiluca forskål (Cnidaria: Scyphozoa).
The activity of Pelagia noctiluca venom was never assessed on cultured cells; therefore, we have evaluated on V79 cells the cytotoxicity, genotoxicity and ATP depletion induced after treatment. Venom did not cause alteration on cell DNA, but showed remarkable cytotoxic properties. With the highest nematocyst concentration (150,000 nematocyst/ml) 74 and 39% cells survived after 1 and 3 h, respectively, when evaluated by Trypan blue. Treated cells showed increased ATP levels during the same time. Preliminary HPLC analyses have showed the occurrence of a protein containing peak.
Mariottini
G L
GL
Dipartimento di Biologia Sperimentale, Ambientale ed Applicata, Università di Genova, Viale Benedetto XV 5, 16132 Genova, Italy. gianlm@unige.it
Sottofattori
E
E
Mazzei
M
M
Robbiano
L
L
Carli
A
A
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003063:Cnidaria / Q000502:physiology*; D003064:Cnidarian Venoms / Q000633:toxicity*; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*
D003064:Cnidarian Venoms; D000255:Adenosine Triphosphate