title: Anoxia/reoxygenation-induced leukocyte-endothelial cell interactions.
abstract: It is increasingly apparent that oxidants can play an important role in mediating specific cell responses and expression of genes involved in degenerative pathophysiologic states, such as inflammation. In particular, oxidant-induced activation of the multisubunit nuclear transcription factor, NFkappaB, has been implicated in the transcriptional upregulation of inflammatory genes like endothelial cell adhesion glycoproteins. A second emerging concept is the recognition that the oxidant effects in cellular and molecular regulation may be mediated by oxidant-induced loss of cellular oxidation-reduction (redox) balance. This review will provide an overview of our current understanding of leukocyte-endothelial cell interactions derived from in vitro studies of endothelial cell monolayers exposed to anoxia/reoxygenation, with specific emphasis on the molecular determinants mediating this inflammatory process and the contribution of reoxygenation-induced cellular redox imbalance to the activation of NFkappaB and the expression of endothelial surface adhesion molecules.
authors.affiliation: First Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan. s-kokura@mpd.biglobe.ne.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kokura
authors.forename: Norimasa
authors.initials: N
authors.lastname: Yoshida
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism; D015687:Cell Hypoxia; D003546:Cysteine Endopeptidases; D004730:Endothelium, Vascular / Q000473:pathology*; D005786:Gene Expression Regulation; D006801:Humans; D007511:Ischemia / Q000378:metabolism / Q000473:pathology*; D007962:Leukocytes / Q000473:pathology*; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors; D016328:NF-kappa B / Q000378:metabolism; D009504:Neutrophils / Q000473:pathology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011480:Protease Inhibitors / Q000494:pharmacology; D046988:Proteasome Endopeptidase Complex; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013601:T-Lymphocytes / Q000473:pathology
chemical_list: D015815:Cell Adhesion Molecules; D009097:Multienzyme Complexes; D016328:NF-kappa B; D011480:Protease Inhibitors; D013438:Sulfhydryl Compounds; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex