Modulation of inflammation by vasoactive intestinal peptide and bombesin: lack of effects on neutrophil apoptosis.
Inhibition of neutrophil apoptosis has been identified as a prominent feature in chronic inflammation, parenchymal damage, and unresolved organ dysfunction. Lung injury animal models suggest that the neuropeptides vasoactive intestinal peptide and bombesin are protective. Therefore, in vitro effects of VIP and bombesin on apoptosis of normal human neutrophils were tested. For measuring effects on cell survival and apoptosis, trypan dye exclusion, colorimetric MTT assay to assess cell survival, and caspase-3 assay and annexin-V binding for analysing apoptosis rates were used. Foetal calf serum, Fas ligand, and tumour necrosis factor-alpha served as modulatory control agents; survival-promoting and apoptosis-inducing activities of the respective agents were confirmed. Vasoactive intestinal peptide and bombesin, however, failed to significantly affect cell death in neutrophils. Data suggest that direct regulation of neutrophil apoptosis is unlikely to be among the mechanisms of lung-protective actions of VIP and bombesin.
Djanani
Angela M
AM
Division of General Internal Medicine, Department of Internal Medicine, University of Innsbruck.
Kähler
Ch M
ChM
D017209:Apoptosis / Q000502:physiology*; D001839:Bombesin / Q000494:pharmacology*; D053148:Caspase 3; D020169:Caspases / Q000097:blood; D002462:Cell Membrane / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology*; D008563:Membrane Lipids / Q000097:blood; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010718:Phosphatidylserines / Q000097:blood; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D008563:Membrane Lipids; D010718:Phosphatidylserines; D014660:Vasoactive Intestinal Peptide; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D001839:Bombesin