Efficient Secretion of Proteins Expressed from Insect Cells Directed by PAM Signal Peptide.
Signal and leading peptide sequences of rat PAM was inserted into the baculovirus transfer vector, and secretion expression plasmids pBACPAG2 and pBacPAI for the fusion gene PABC-hGRF and PABC-IGF-I were constructed, respectively. By cotransfection with linear genomic DNA of modified Autographa californica nuclear polyhedrosis virus (BacPAK6) and homologous recombination, the recombinant AcNPV, BacPAG and BacPAI, were obtained and identified. Fusion proteins PABC-hGRF and PABC-IGF-I were secreted efficiently from Sf21 cells infected with BacPAG and BacPAI, respectively, and those fusion protein could be purified efficiently by IgG affinity column.
Jiang
Zhi-Hong
ZH
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. boliang@server.shcnc.ac.cn
Yang
Yu-Hong
YH
Wu
Xiang-Fu
XF
Li
Bo-Liang
BL
Wang
De-Bao
DB