Detection of Rhodococcus equi by polymerase chain reaction using species-specific nonproprietary primers.
Species-specific primers for the polymerase chain reaction (PCR) for the detection of Rhodococcus equi were developed. These primers were based on unique DNA fragments produced from R. equi reference strains and field isolates. Following random amplification of polymorphic DNA from R. equi and R. rhodochrous with a set of 40 arbitrary 10-base pair (bp) primers, a pair of species-specific primers was designed to detect a unique 700-bp fragment of R. equi chromosomal DNA. This PCR product was limited to R. equi and was not detectable in other Rhodococcus species or in a panel of additional gram-positive and gram-negative bacteria.
Arriaga
José Miguel
JM
Department of Large Animal Medicine, College of Veterinary Medicine, Texas A&M University, College Station 77843-4475, USA.
Cohen
Noah D
ND
Derr
James N
JN
Chaffin
M Keith
MK
Martens
Ronald J
RJ
D000193:Actinomycetales Infections / Q000175:diagnosis / Q000662:veterinary*; D000818:Animals; D017931:DNA Primers; D006734:Horse Diseases / Q000175:diagnosis* / Q000235:genetics; D006736:Horses; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D016929:Rhodococcus equi / Q000235:genetics* / Q000472:pathogenicity; D012680:Sensitivity and Specificity
D017931:DNA Primers