Isolation and characterization of an angiogenin-like protein from goat plasma.
Angiogenin, a blood vessel inducing protein has been implicated in wound healing and tumour progression. First isolated from human carcinoma cells, it has been subsequently isolated from human, bovine, rabbit, pig and mouse sera and bovine milk. This study reports the isolation of an angiogenic-like protein from goat plasma. The ribonucleolytic activity has been followed by yeast transfer RNA (tRNA) degradation using spectrophotometric and denaturing polyacrylamide gel electrophoresis methods. The chorioallantoic membrane (CAM) assay has been implemented to study its angiogenic activity. The presence of this protein has also been confirmed by strong binding with placental Ribonuclease Inhibitor (PRI).
Maiti
Tushar Kanti
TK
Department of Chemistry, Indian Institute of Technology, Kharagpur, West Bengal, 721 302, India.
Dasgupta
Swagata
S
D043925:Angiogenesis Inducing Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D002642:Chick Embryo; D002845:Chromatography; D005321:Extraembryonic Membranes / Q000187:drug effects; D006041:Goats / Q000097:blood*; D006801:Humans; D018919:Neovascularization, Physiologic; D012343:RNA, Transfer / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015003:Yeasts / Q000235:genetics
D043925:Angiogenesis Inducing Agents; D012343:RNA, Transfer; C046366:angiogenin; D012259:Ribonuclease, Pancreatic