title: In vitro aggregation of the regulated secretory protein chromogranin A.
abstract: Aggregation chaperones, consisting of secretory proteins that contain a hexa-histidine epitope tag, enhance the calcium-induced aggregation of regulated secretory proteins and their sorting to secretory granules. The goal of this study was to gain a better understanding of this unusual aggregation mechanism. Hexa-histidine-epitope-tagged secreted alkaline phosphatase, an aggregation chaperone, enhanced the in vitro aggregation of chromogranin A in the presence of calcium, but not in the presence of magnesium or other divalent cations. As an exception, chromogranin was completely aggregated by zinc, even in the absence of the aggregation chaperone. In addition, fluorescence spectroscopy of the aggregation reaction mixture showed an increase in fluorescence intensity consistent with the formation of protein aggregates. The calcium-induced aggregation of chromogranin A was completely inhibited by 0.2% Triton X-100, suggesting that it involves hydrophobic interactions. In contrast, the detergent did not affect chaperone-enhanced aggregation, suggesting that this aggregation does not depend on hydrophobic interactions. EDTA-treated chaperone did not enhance chromogranin A aggregation, indicating that divalent cations are necessary for chaperone action. Although the structure of the aggregation chaperone was not important, the size of the chaperone was. Thus the free His-hexapeptide could not substitute for the aggregation chaperone. Based on these results, we propose that the hexa-histidine tag, in the context of a polypeptide, acts as a divalent cation-dependent nucleation site for chromogranin A aggregation.
authors.affiliation: Department of Periodontics, Endodontics and Dental Hygiene, Health Sciences Center, University of Louisville, Louisville, KY 40292, U.S.A.
authors.forename: Renu K
authors.initials: RK
authors.lastname: Jain
authors.forename: Wen Tzu
authors.initials: WT
authors.lastname: Chang
authors.forename: Chitta
authors.initials: C
authors.lastname: Geetha
authors.forename: Paul B M
authors.initials: PB
authors.lastname: Joyce
authors.forename: Sven-Ulrik
authors.initials: SU
authors.lastname: Gorr
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002412:Cations; D002417:Cattle; D053379:Chromogranin A; D002864:Chromogranins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D004492:Edetic Acid / Q000737:chemistry / Q000494:pharmacology; D000939:Epitopes / Q000235:genetics / Q000378:metabolism; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism; D017830:Octoxynol / Q000737:chemistry / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D015032:Zinc / Q000737:chemistry / Q000378:metabolism
chemical_list: D002412:Cations; D053379:Chromogranin A; D002864:Chromogranins; D000939:Epitopes; D018832:Molecular Chaperones; D011994:Recombinant Proteins; D006639:Histidine; D017830:Octoxynol; D004492:Edetic Acid; D000469:Alkaline Phosphatase; D008274:Magnesium; D015032:Zinc