title: Modulation by leptin, insulin and corticosterone of oleoyl-estrone synthesis in cultured 3T3 L1 cells.
abstract: Preadipocytes (3T3 L1) were used between 7 and 14 days after differentiation; they were incubated with 44 nM 3H-esterone. The medium was supplemented with 1 microM recombinant murine leptin, 10 nM recombinant human insulin, or 1 microM corticosterone for up to 72 hr. In a second series of experiments, cells were incubated for 48 hr with different concentrations of leptin, insulin or corticosterone, and compared with controls (plain medium). Cells were harvested, washed in buffer and homogenized, and protein was measured. Lipid extracts of cell homogenates were used for HPLC; the label distribution in free and acylestrone peaks was measured. Overall uptake of estrone (i.e., the sum of free and acylestrone) by cells was not affected by leptin or corticosterone, but strongly reduced by insulin. Leptin and corticosterone increased the synthesis of acyl-esterone in a dose- and time-dependent way. Insulin decreased acyl-estrone synthesis at low concentrations and with little change over time. The results suggest that control of oleoyl-estrone deposition in adipocytes is modulated in at least two distinct steps: (a) estrone uptake, affected by insulin in the physiological range; and (b) synthesis of oleoyl-estrone from cell estrone. The latter may be affected by insulin, but leptin and corticosterone enhance the process.
authors.affiliation: Centre Especial de Recerca en Nutrició i Ciència dels Aliments, Facultat de Biologia, Universitat de Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Esteve
authors.forename: P
authors.initials: P
authors.lastname: Savall
authors.forename: J
authors.initials: J
authors.lastname: Virgilli
authors.forename: J A
authors.initials: JA
authors.lastname: Fernández-López
authors.forename: X
authors.initials: X
authors.lastname: Remesar
authors.forename: M
authors.initials: M
authors.lastname: Alemany
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003345:Corticosterone / Q000378:metabolism*; D004970:Estrone / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism*; D051379:Mice; D009829:Oleic Acids / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000378:metabolism; D013223:Statistics as Topic
chemical_list: D007328:Insulin; D020738:Leptin; D009829:Oleic Acids; D011994:Recombinant Proteins; D004970:Estrone; C108709:oleoyl-estrone; D003345:Corticosterone