Sequence Analysis of the Trailer Region of Wheat Rosette Stunt Virus Genomic RNA.
According to a highly conserved 8 amino acid sequence of L proteins in Rhabdoviridae, a 24-mer degenerate oligonucleotide primer was designed to amplify the 5' terminus of the genomic RNA of wheat rosette stunt virus (WRSV), a plant rhabdovirus, by anchored PCR. The complete nucleotide sequence of the 5' trailer in WRSV genomic RNA was determined by deoxynucleotide sequencing of cloned cDNA derived from the anchored PCR products.
Ye
Yong-Jun
YJ
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. gongzx@sunm.shcnc.ac.cn
Gong
Zu-Xun
ZX