title: Differentiation of monoblastic cell line UG3 into leukemic dendritic cells.
abstract: Dendritic cells (DCs) are known to be generated from leukemic clone from patients with acute and chronic leukemia when cultured in the presence of combination of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF-alpha) and interleukin-4. However, there have been few reports that showed DCs could be effectively differentiated from human leukemia cell lines. In this study, we have shown that a human monoblastic cell line, UG3, was inducible to differentiate into DCs in the presence of GM-CSF and TNF-alpha along monocyte-macrophage lineage. These DCs, consistently displayed dendritic morphology, phenotypes and allogeneic T-cell stimulating capacity. UG3 cells thus may represent a suitable model to further elucidate characteristics of DC differentiation.
authors.affiliation: Second Department of Internal Medicine, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
authors.forename: Nobuharu
authors.initials: N
authors.lastname: Fujii
authors.forename: Takashi
authors.initials: T
authors.lastname: Ikeda
authors.forename: Kazuma
authors.initials: K
authors.lastname: Ikeda
authors.forename: Akio
authors.initials: A
authors.lastname: Hiraki
authors.forename: Kimihiko
authors.initials: K
authors.lastname: Kawakami
authors.forename: Kozo
authors.initials: K
authors.lastname: Masuda
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Maeda
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Hatake
authors.forename: Kazuo
authors.initials: K
authors.lastname: Motoyoshi
authors.forename: Mine
authors.initials: M
authors.lastname: Harada
authors.forename: Mitsune
authors.initials: M
authors.lastname: Tanimoto
mesh_terms: D017951:Antigen Presentation / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000276:immunology; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor