title: Effects of diets enriched in saturated (palmitic), monounsaturated (oleic), or trans (elaidic) fatty acids on insulin sensitivity and substrate oxidation in healthy adults.
abstract: OBJECTIVE
  Diets high in total and saturated fat are associated with insulin resistance. This study examined the effects of feeding monounsaturated, saturated, and trans fatty acids on insulin action in healthy adults.
  
  
  METHODS
  A randomized, double-blind, crossover study was conducted comparing three controlled 4-week diets (57% carbohydrate, 28% fat, and 15% protein) enriched with different fatty acids in 25 healthy men and women. The monounsaturated fat diet (M) had 9% of energy as C18:1cis (oleic acid). The saturated fat diet (S) had 9% of energy as palmitic acid, and the trans fatty acid diet (T) had 9% as C18:1trans. Body weight was kept constant throughout the study. After each diet period, insulin pulsatile secretion, insulin sensitivity index (S(I)) by the minimal model method, serum lipids, and fat oxidation by indirect calorimetry were measured.
  
  
  RESULTS
  Mean S(I) for the M, S, and T diets was 3.44 +/- 0.26, 3.20 +/- 0.26, and 3.40 +/- 0.26 x 10(-4) min(-1). microU(-1). ml(-1), respectively (NS). S(I) decreased by 24% on the S versus M diet in overweight subjects but was unchanged in lean subjects (NS). Insulin secretion was unaffected by diet, whereas total and HDL cholesterol increased significantly on the S diet. Subjects oxidized the least fat on the M diet (26.0 +/- 1.5 g/day) and the most fat on the T diet (31.4 +/- 1.5 g/day) (P = 0.02).
  
  
  CONCLUSIONS
  Dietary fatty acid composition significantly influenced fat oxidation but did not impact insulin sensitivity or secretion in lean individuals. Overweight individuals were more susceptible to developing insulin resistance on high-saturated fat diets.
authors.affiliation: Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, Louisiana 70808, USA.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Lovejoy
authors.forename: Steven R
authors.initials: SR
authors.lastname: Smith
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Champagne
authors.forename: Marlene M
authors.initials: MM
authors.lastname: Most
authors.forename: Michael
authors.initials: M
authors.lastname: Lefevre
authors.forename: James P
authors.initials: JP
authors.lastname: DeLany
authors.forename: Yvonne M
authors.initials: YM
authors.lastname: Denkins
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Rood
authors.forename: Johannes
authors.initials: J
authors.lastname: Veldhuis
authors.forename: George A
authors.initials: GA
authors.lastname: Bray
mesh_terms: D000328:Adult; D001835:Body Weight; D018592:Cross-Over Studies; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007333:Insulin Resistance / Q000502:physiology*; D000078790:Insulin Secretion; D008297:Male; D019301:Oleic Acid / Q000008:administration & dosage*; D009829:Oleic Acids; D010084:Oxidation-Reduction; D019308:Palmitic Acid / Q000008:administration & dosage*
chemical_list: D004042:Dietary Fats, Unsaturated; D007328:Insulin; D009829:Oleic Acids; D019301:Oleic Acid; D019308:Palmitic Acid; C011459:elaidic acid