Fermentation of enzymatically saccharified sunflower stalks for ethanol production and its scale up.
Pretreated sunflower stalks saccharified with a Trichoderma reesei Rut-C 30 cellulase showed 57.8% saccharification. Enzyme hydrolysate concentrated to 40 g/l reducing sugars was fermented under optimum conditions of fermentation time (24 h), pH (5.0), temperature (30 degrees C) and inoculum size (3% v/v) and, showed a maximum ethanol yield of 0.444 g/g ethanol. Ethanol production scaled up in a 1 l and a 15 l fermenter under optimum conditions revealed maximum ethanol yields of 0.439 and 0.437 g/g respectively.
Sharma
Sanjeev K
SK
Department of Microbiology, College of Basic Sciences and Humanities, Punjab Agricultural University, India.
Kalra
Krishan L
KL
Grewal
Harmeet S
HS
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002478:Cells, Cultured; D002480:Cellulase / Q000378:metabolism*; D003470:Culture Media; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006368:Helianthus / Q000033:anatomy & histology; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism; D013227:Steam; D013696:Temperature; D013997:Time Factors; D014242:Trichoderma / Q000201:enzymology
D003470:Culture Media; D013227:Steam; D000431:Ethanol; D002480:Cellulase; D010100:Oxygen