Frequent detection of human papilloma viruses in cervical dysplasia by PCR single-strand DNA-conformational polymorphism analysis.
BACKGROUND
To clarify the pathogenicity of multiple human papilloma virus (HPV) infection, we applied SSCP (single-strand DNA conformation polymorphism) analysis for cervical neoplastic lesions.


METHODS
Two hundred and sixty-six cervical swab specimens from normal cervix (n=64), cervical dysplasia (n=95), carcinoma in situ (n=79) and cervical cancer (n=28), were studied by nested PCR-SSCP analysis using L1 consensus primers.


RESULTS
In 95 samples of cervical dysplasia, HPV infection was detected in 98.9% (94 out of 95), multiple HPV infection was detected in 38.3% (36 out of 94). In 19 squamous cell carcinomas (SCC) and 9 adenocarcinomas, the detection rate of HPV infection was 84.2% (16 out of 19) and 55.6% (5 out of 9), respectively, and all HPV-positive cases showed infection of a single HPV, among which HPV 16 occupied 68.6% (11 out of 16) in SCC and HPV 18 occupied 100% (5 out of 5) in adenocarcinoma.


CONCLUSIONS
Multiple HPV infections may be concerned with pathogenicity in cervical dysplasia; however, the single infection with only a few HPV types, such as type 16 in SCC and type 18 in adenocarcinoma, may play a role in cervical carcinogenesis.
Nakagawa
Hiroyuki
H
Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan. hnakagaw@mc.med.keio.ac.jp
Sugano
Kokichi
K
Fujii
Takuma
T
Kubushiro
Kaneyuki
K
Tsukazaki
Katsumi
K
Nozawa
Shiro
S
D000230:Adenocarcinoma / Q000821:virology; D002278:Carcinoma in Situ / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000821:virology; D004279:DNA, Viral / Q000145:classification / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D014412:Tumor Virus Infections / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000821:virology*
D004279:DNA, Viral