Helicobacter pylori SabA adhesin in persistent infection and chronic inflammation.
Helicobacter pylori adherence in the human gastric mucosa involves specific bacterial adhesins and cognate host receptors. Here, we identify sialyl-dimeric-Lewis x glycosphingolipid as a receptor for H. pylori and show that H. pylori infection induced formation of sialyl-Lewis x antigens in gastric epithelium in humans and in a Rhesus monkey. The corresponding sialic acid-binding adhesin (SabA) was isolated with the "retagging" method, and the underlying sabA gene (JHP662/HP0725) was identified. The ability of many H. pylori strains to adhere to sialylated glycoconjugates expressed during chronic inflammation might thus contribute to virulence and the extraordinary chronicity of H. pylori infection.
Mahdavi
Jafar
J
Department of Odontology/Oral Microbiology, Umeå University, SE-901 87 Umeå, Sweden.
Sondén
Berit
B
Hurtig
Marina
M
Olfat
Farzad O
FO
Forsberg
Lina
L
Roche
Niamh
N
Angstrom
Jonas
J
Larsson
Thomas
T
Teneberg
Susann
S
Karlsson
Karl-Anders
KA
Altraja
Siiri
S
Wadström
Torkel
T
Kersulyte
Dangeruta
D
Berg
Douglas E
DE
Dubois
Andre
A
Petersson
Christoffer
C
Magnusson
Karl-Eric
KE
Norberg
Thomas
T
Lindh
Frank
F
Lundskog
Bertil B
BB
Arnqvist
Anna
A
Hammarström
Lennart
L
Borén
Thomas
T
D018829:Adhesins, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001422:Bacterial Adhesion; D002240:Carbohydrate Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D005753:Gastric Mucosa / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D005756:Gastritis / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D005798:Genes, Bacterial; D006001:Glycoconjugates / Q000378:metabolism; D016481:Helicobacter Infections / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D016256:Lewis X Antigen / Q000378:metabolism*; D008253:Macaca mulatta; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism; D000080506:Sialyl Lewis X Antigen
D018829:Adhesins, Bacterial; C400429:BabA protein, Helicobacter pylori; D002352:Carrier Proteins; D006001:Glycoconjugates; D016256:Lewis X Antigen; D009844:Oligosaccharides; D012794:Sialic Acids; D000080506:Sialyl Lewis X Antigen; C404741:galactopyranosyl-1-3-galactopyranosyl-1-3(4)-N-acetylglucosamine