Development of a polymerase chain reaction and restriction typing assay for the diagnosis of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections.
A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.
De-Giuli
Luciana
L
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna Bruno Ubertini, Sezione diagnostica di Pavia, Italy.
Magnino
Simone
S
Vigo
Pier Giorgio
PG
Labalestra
Iris
I
Fabbi
Massimo
M
D000818:Animals; D002417:Cattle; D004279:DNA, Viral / Q000032:analysis; D003937:Diagnosis, Differential; D006561:Herpes Simplex / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D001911:Herpesvirus 2, Bovine / Q000235:genetics*; D026021:Herpesvirus 4, Bovine / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*
D004279:DNA, Viral