Microbial cell wall product contamination of bedding may induce pulmonary inflammation in rats.
To test the hypothesis that airborne microbial cell wall components could induce an inflammatory response in the lungs, measurements were made of the amounts of bacterial endotoxin and (1-->3)-beta-D-glucan in laboratory animal bedding materials. Groups of rats were exposed by inhalation to airborne endotoxin, (1-->3)-beta-D-glucan or a combination of the two for 5 weeks. The results demonstrated that measurable amounts of endotoxin and (1-->3)-beta-D-glucan could be detected in the different bedding materials. In contrast to animals at delivery, those kept on bedding for 5 weeks showed moderate inflammatory reactions in the lung. These were most pronounced among animals exposed to endotoxin and (1-->3)-beta-D-glucan. The results suggest that further studies need to be undertaken to elucidate the role of microbial cell wall products in the development of inflammatory lung responses among research animals.
Ewaldsson
B
B
AstraZeneca R&D, Laboratory Animal Resources, Mölndal, Sweden.
Fogelmark
B
B
Feinstein
R
R
Ewaldsson
L
L
Rylander
R
R
D000280:Administration, Inhalation; D000336:Aerosols; D000393:Air Pollutants / Q000032:analysis / Q000276:immunology*; D000542:Alveolitis, Extrinsic Allergic / Q000209:etiology* / Q000473:pathology; D000818:Animals; D001512:Bedding and Linens / Q000382:microbiology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004306:Dose-Response Relationship, Immunologic; D004866:Equipment Contamination; D004926:Escherichia coli; D005260:Female; D005936:Glucans / Q000008:administration & dosage / Q000032:analysis / Q000276:immunology*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000032:analysis / Q000276:immunology*; D008168:Lung / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D047071:beta-Glucans
D000336:Aerosols; D000393:Air Pollutants; D005936:Glucans; D008070:Lipopolysaccharides; D047071:beta-Glucans; C033363:beta-1,3-glucan