Analysis of C-terminal Amidating Structure and Determination of Amidating Enzyme Activity by Using Capillary Electrophoresis.
Based on the difference in charge and hydrophobicity between amidating moiety and the carboxyl group in a given buffer solution, a new method to analyze the C-terminal amidating structure and the amidating enzyme activity by using capillary electrophoresis was established.
Jiang
Zhi-Hong
ZH
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
Tu
Hong-Min
HM
Yang
Yu-Hong
YH
Xia
Qi-Chang
QC
Li
Bo-Liang
BL
Wang
De-Bao
DB