title: Cell-free human T-cell leukemia virus type 1 binds to, and efficiently enters mouse cells.
abstract: Human T-cell leukemia virus type 1 (HTLV-1) is an etiologic agent of adult T-cell leukemia / lymphoma and other HTLV-1-associated diseases. However, the interaction between HTLV-1 and T cells in the pathogenesis of these diseases is poorly understood. Mouse cells have been reported to be resistant to cell-free HTLV-1 infection. However, we recently reported that HTLV-1 DNA could be observed 24 h after cell-free HTLV-1 infection of mouse cell lines. To understand HTLV-1 replication in these cells in detail, we concentrated the virus produced from c77 feline kidney cell line and established an efficient infection system. The amounts of adsorption of HTLV-1 are larger in mouse T cell lines, EL4 and RLm1, than those in human T cell lines, Molt4 and HUT78, and are similar to that in human kidney cell line, 293T. Unexpectedly, however, the amounts of entry of HTLV-1 are about 10-fold larger in the two mouse cell lines than those in the three human cell lines employed. Moreover, viral DNA was detectable from 1 h in EL4 and RLm1 cells, but only from 2 - 3 h in 293T, Molt4 and HUT78 cells. However, the amount of viral DNA in EL4 cells became smaller than that in Molt4 cells. HTLV-1 expression could be detected until day 1 - 2 in RLm1 and EL4 cells, and until day 4 in Molt4 cells. Our results suggest that mouse cell experiments would give useful information to dissect the early steps of cell-free HTLV-1 infection.
authors.affiliation: Department of Biochemistry and Molecular Oncology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan. m-miwa@md.tsukuba.ac.jp
authors.forename: Binlian
authors.initials: B
authors.lastname: Sun
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nitta
authors.forename: Momoko
authors.initials: M
authors.lastname: Shoda
authors.forename: Masakazu
authors.initials: M
authors.lastname: Tanaka
authors.forename: Shuji
authors.initials: S
authors.lastname: Hanai
authors.forename: Hiroo
authors.initials: H
authors.lastname: Hoshino
authors.forename: Masanao
authors.initials: M
authors.lastname: Miwa
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D002474:Cell-Free System; D004279:DNA, Viral / Q000378:metabolism; D015368:Human T-lymphotropic virus 1 / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000821:virology*; D013997:Time Factors
chemical_list: D004279:DNA, Viral