title: Serologic and molecular detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (human granulocytic ehrlichiosis agent) in Korean patients.
abstract: Sera from 491 Korean patients with acute febrile diseases were tested for Ehrlichia chaffeensis and Anaplasma phagocytophila antibodies by indirect immunofluorescence assay (IFA), Western blotting, and TaqMan real-time PCR. Overall, 0.4% of sera reacted with E. chaffeensis, and 1.8% reacted with A. phagocytophila in IFAs. This is the first report of detection of antibodies to A. phagocytophila and E. chaffeensis in Korea and suggests the presence of A. phagocytophila and E. chaffeensis or antigenically similar species.
authors.affiliation: Department of Veterinary Internal Medicine and Clinical Pathology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561-756, Korea.
authors.forename: Eun-jeong
authors.initials: EJ
authors.lastname: Heo
authors.forename: Jin-ho
authors.initials: JH
authors.lastname: Park
authors.forename: Ja-ryong
authors.initials: JR
authors.lastname: Koo
authors.forename: Man-suk
authors.initials: MS
authors.lastname: Park
authors.forename: Mi-yeoun
authors.initials: MY
authors.lastname: Park
authors.forename: J Stephen
authors.initials: JS
authors.lastname: Dumler
authors.forename: Joon-seok
authors.initials: JS
authors.lastname: Chae
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000709:Anaplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000097:blood*; D015153:Blotting, Western; D018469:Ehrlichia chaffeensis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007723:Korea / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D019914:Taq Polymerase