title: Optimization strategies for HPLC enantioseparation of racemic drugs using polysaccharides and macrocyclic glycopeptide antibiotic chiral stationary phases.
abstract: The chiral resolution by high performance liquid chromatography (HPLC) is controlled by a number of parameters. The optimization of HPLC parameters is an important issue in chiral resolution. This review discusses the optimization of HPLC conditions for the chiral resolution of racemic drugs on polysaccharides and macrocyclic glycopeptide antibiotic chiral stationary phases (CSPs). The most important parameters discussed are composition of mobile phase, pH of mobile phase, flow rate, temperature and effect of other parameters.
authors.affiliation: Biological and Medical Research Department (MBC-03), King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. enein@kfshrc.edu.sa
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
authors.forename: Imran
authors.initials: I
authors.lastname: Ali
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006020:Glycopeptides; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D011134:Polysaccharides / Q000737:chemistry*; D013237:Stereoisomerism; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D002021:Buffers; D006020:Glycopeptides; D004364:Pharmaceutical Preparations; D011134:Polysaccharides