title: SEM study on the effect of two different demineralization methods with saturated tetracycline hydrochloride on diseased root surfaces.
abstract: The scanning electron microscope was used to evaluate the dentin surface of diseased teeth subjected to two methods of conditioning with tetracycline hydrochloride (TTC) for 1 and 4 minutes respectively. Five groups of twelve specimens each received root planing (control); were immersed in TTC for 1 and 4 minutes; and burnished in TTC for 1 and 4 minutes. Control specimens exhibited an amorphous irregular surface smear layer. TTC, irrespective of the method used, was effective in removing the smear layer. Immersion in TTC for 1 minute revealed obstructed dentinal tubules, while burnishing for 1 minute revealed the presence of collagen fibrils. The 4-minute application of TTC, irrespective of the conditioning method used, showed wide exposed dentinal tubules. No significant difference between Groups III, IV, and V was noted (p>0.05).
authors.affiliation: Department of Preventive Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi Arabia. nbabay@ksu.edu.sa
authors.forename: N
authors.initials: N
authors.lastname: Babay
mesh_terms: D000704:Analysis of Variance; D003648:Decalcification Technique; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure*; D004305:Dose-Response Relationship, Drug; D024022:Fibrillar Collagens / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010518:Periodontitis / Q000628:therapy*; D016745:Root Planing; D016369:Smear Layer; D018709:Statistics, Nonparametric; D013752:Tetracycline / Q000008:administration & dosage*; D013997:Time Factors; D014092:Tooth Root / Q000187:drug effects*
chemical_list: D024022:Fibrillar Collagens; D013752:Tetracycline