Epigenetic modification of plants with systemic RNA viruses.
Knowledge of gene function is critical to the development of new plant traits for improved agricultural and industrial applications. Viral expression vectors offer a rapid and proven method to provide epigenetic expression of foreign sequences throughout infected plants. Expression of these sequences from viral vectors can lead to gain- or loss-of-function phenotypes, allowing gene function to be determined by phenotypic or biochemical effects in the infected plant. Tobacco mosaic virus and barley stripe mosaic virus expression vectors have been developed to express foreign gene sequences in dicotyledonous and monocotyledonous hosts, respectively. Large-scale application of both viral vector systems for gene function discovery in Nicotiana and barley hosts resulted in high infection rates and produced distinctive visual phenotypes in approximately 5% of transfected plants. Novel genes expressing potential herbicide target proteins in addition to genes promoting stem elongation, leaf development and apical dominance were identified in the large-scale screening. This report illustrates the adaptability of viral vectors for gene function discovery in higher plants.
Fitzmaurice
Wayne P
WP
Large Scale Biology Corp, Vacaville, CA 95688, USA.
Holzberg
Steve
S
Lindbo
John A
JA
Padgett
Hal S
HS
Palmer
Kenneth E
KE
Wolfe
Gershon M
GM
Pogue
Gregory P
GP
D016373:DNA, Antisense / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D005822:Genetic Vectors; D001467:Hordeum / Q000235:genetics / Q000502:physiology; D016366:Open Reading Frames; D010641:Phenotype; D030821:Plants, Genetically Modified; D012328:RNA Viruses / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000502:physiology; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014158:Transcription, Genetic
D016373:DNA, Antisense