title: Isolation of eastern equine encephalitis virus and West Nile virus from crows during increased arbovirus surveillance in Connecticut, 2000.
abstract: The emergence of the West Nile virus (WNV) in the northeastern United States has drawn emphasis to the need for expanded arbovirus surveillance in Connecticut. Although the state of Connecticut began a comprehensive mosquito-screening program in 1997, only since 1999 have there been efforts to determine the prevalence of arboviruses in bird populations in this state. Herein, we report on our results of an arbovirus survey of 1,704 bird brains. Included in this report are the first known isolations of eastern equine encephalitis virus (EEEV) from crows and data on the geographic and temporal distribution of 1,092 WNV isolations from crow species. Moreover, these nine isolations of EEEV identify regions of Connecticut where the virus is rarely found. With the exception of WNV and EEEV, no other arboviruses were isolated or detected. Taken together, these data illustrate the distribution of avian borne EEEV and WNV in 2000 and support the need for ongoing avian arbovirus surveillance in Connecticut.
authors.affiliation: Connecticut Department of Public Health Laboratory, Biological Sciences, Hartford 06144, USA. william.beckwith@po.state.ct.us
authors.forename: William H
authors.initials: WH
authors.lastname: Beckwith
authors.forename: Stanley
authors.initials: S
authors.lastname: Sirpenski
authors.forename: Richard A
authors.initials: RA
authors.lastname: French
authors.forename: Randall
authors.initials: R
authors.lastname: Nelson
authors.forename: Donald
authors.initials: D
authors.lastname: Mayo
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000453:epidemiology / Q000821:virology*; D001921:Brain / Q000821:virology; D003237:Connecticut / Q000453:epidemiology; D004663:Encephalitis Virus, Eastern Equine / Q000235:genetics / Q000302:isolation & purification*; D020242:Encephalomyelitis, Eastern Equine / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D011159:Population Surveillance; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020308:Songbirds / Q000821:virology*; D014776:Virus Cultivation; D014901:West Nile Fever / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014902:West Nile virus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral