title: Conventional mechanical ventilation of healthy lungs induced pro-inflammatory cytokine gene transcription.
abstract: We investigated the potential inflammatory reaction induced by mechanical ventilation (MV) using 10 ml/kg tidal volume and no positive end-expiratory pressure (PEEP) in control (C, n = 8), spontaneously breathing (SB, n = 12) and mechanically ventilated (MV, n = 12) rabbits with normal lungs. After 6 h (MV and SB groups) or immediately (C group), lungs were removed for measurement of wet-to-dry (W/D) weight ratio and for bronchoalveolar lavage (BAL). Pulmonary mechanics were also studied. MV animals developed a modest but significant (P < 0.01) impairment of arterial blood oxygenation and had higher W/D lung weight ratio than C ones. In MV group, BAL macrophage count was greater (P < 0.05) than in SB one. MV induced an upregulation of MCP-1, TNF-alpha, and IL-1beta gene transcription (mRNAs), without significant elevation of the corresponding protein cytokines in the BAL supernatant, except for MCP-1 (P < 0.05). These data suggest that MV, even using moderate tidal volume, elicits a pro-inflammatory stimulus to the lungs.
authors.affiliation: Laboratoire de Physiopathologie Respiratoire (EA 2201), Institut Jean Roche, Faculté de Médecine, Université de la Mediterranée, Bd P Dramard, 13916 Marseille cedex 20, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Brégeon
authors.forename: Antoine
authors.initials: A
authors.lastname: Roch
authors.forename: Stéphane
authors.initials: S
authors.lastname: Delpierre
authors.forename: Eric
authors.initials: E
authors.lastname: Ghigo
authors.forename: Amapola
authors.initials: A
authors.lastname: Autillo-Touati
authors.forename: Osamu
authors.initials: O
authors.lastname: Kajikawa
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Martin
authors.forename: Jérôme
authors.initials: J
authors.lastname: Pugin
authors.forename: Henry
authors.initials: H
authors.lastname: Portugal
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Auffray
authors.forename: Yves
authors.initials: Y
authors.lastname: Jammes
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D007958:Leukocyte Count; D008168:Lung / Q000378:metabolism* / Q000503:physiopathology; D011014:Pneumonia / Q000235:genetics / Q000503:physiopathology; D011659:Pulmonary Gas Exchange / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D012121:Respiration, Artificial; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D012333:RNA, Messenger