CP3 is involved in negative regulation of phytochrome A signalling in Arabidopsis.
Several mutants with altered phytochrome A (phyA) signalling have been identified in screenings under continuous far-red light (FR). The latter protocol could preclude the identification of mutants affected in the signalling pathway that operates even under transient phyA activation, compared to the high-irradiance response (HIR) pathway that requires continuous FR. Since some photomorphogenic mutants show shoot-height phenotypes, the screening was conducted on dwarf mutants of Arabidopsis thaliana (L.) Heynh. from the ABRC stocks grown under hourly FR pulses. The dwarf mutant cp3 (compacta 3) showed normal hypocotyl length and folded cotyledons in darkness but enhanced hypocotyl-growth inhibition and cotyledon unfolding under pulsed FR. The HIR and the response mediated by phyB were not affected. Under pulsed FR, seed germination and blocking of greening upon transfer to white light were enhanced in cp3. PHYA levels were normal in cp3. The phenotype under pulsed FR but not the adult phenotype required phyA. We propose that CP3 is involved in the negative regulation of the signalling pathway that saturates with transient activation of phyA.
Quinn
Matías H
MH
IFEVA, Facultad de Agronomía, Universidad de Buenos Aires and CONICET, Av. San Martín 4453, 1417-Buenos Aires, Argentina.
Oliverio
Karina
K
Yanovsky
Marcelo J
MJ
Casal
Jorge J
JJ
D000222:Adaptation, Physiological / Q000235:genetics; D000872:Anthocyanins / Q000096:biosynthesis / Q000528:radiation effects; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D029681:Arabidopsis Proteins; D002734:Chlorophyll / Q000096:biosynthesis / Q000528:radiation effects; D018548:Cotyledon / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D003624:Darkness; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D008027:Light; D009154:Mutation; D010641:Phenotype; D017440:Photoperiod; D010786:Photoreceptor Cells; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D010834:Phytochrome / Q000378:metabolism*; D051942:Phytochrome A; D051939:Phytochrome B; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D015398:Signal Transduction / Q000502:physiology* / Q000528:radiation effects; D014157:Transcription Factors
D000872:Anthocyanins; D029681:Arabidopsis Proteins; C497973:PHYA protein, Arabidopsis; C497952:PHYB protein, Arabidopsis; D051942:Phytochrome A; D014157:Transcription Factors; D010834:Phytochrome; D051939:Phytochrome B; D002734:Chlorophyll