title: [Highly efficient site-directed RNA cleavage by imidazole-containing conjugates of antisense oligonucleotides].
abstract: A method has been suggested for the synthesis of conjugates of oligodeoxyribonucleotides with chemical constructs mimicking ribonuclease A active center for directed fragmentation of RNA. The method is based on the sequential addition of linker group, 9-(methylamino)anthracene, to 5' or 3' terminal phosphate of oligonucleotide and then imidazole-containing construct by cycloaddition reaction. The conjugates of oligonucleotides complementary to regions 44-61 (2B-R) and 60-76 (1C-R) of yeast phenylalanine tRNA demonstrated ability to cleave tRNA(Phe) under physiological conditions preferably at the sole phosphodiester bond (C63-A64 for 2B-R and C56-G57 for 1C-R, respectively). The half-time of tRNA(Phe) hydrolysis in the presence of 2B-R conjugate was 30 min at 2B-R concentration of 10 microM and several minutes at conjugate concentration of 50 microM.
authors.affiliation: Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
authors.forename: N G
authors.initials: NG
authors.lastname: Beloglazova
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Epanchintsev
authors.forename: V N
authors.initials: VN
authors.lastname: Sil'nikov
authors.forename: M A
authors.initials: MA
authors.lastname: Zenkova
authors.forename: V V
authors.initials: VV
authors.lastname: Vlasov
mesh_terms: D001483:Base Sequence; D001671:Biochemistry / Q000379:methods*; D006868:Hydrolysis; D007093:Imidazoles / Q000737:chemistry*; D007700:Kinetics; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012360:RNA, Transfer, Phe / Q000737:chemistry; D012259:Ribonuclease, Pancreatic / Q000737:chemistry
chemical_list: D007093:Imidazoles; D016376:Oligonucleotides, Antisense; D012360:RNA, Transfer, Phe; D012313:RNA; C029899:imidazole; D012259:Ribonuclease, Pancreatic