title: Dual effects of phytoestrogens result in u-shaped dose-response curves.
abstract: Endocrine disruptors can affect the endocrine system without directly interacting with receptors, for example, by interfering with the synthesis or metabolism of steroid hormones. The aromatase that converts testosterone to 17beta-estradiol is a possible target. In this paper we describe an assay that simultaneously detects aromatase inhibition and estrogenicity. The principle is similar to that of other MCF-7 estrogenicity assays, but with a fixed amount of testosterone added. The endogenous aromatase activity in MCF-7 cells converts some of the testosterone to 17beta-estradiol, which is assayed by quantifying differences in the expression level of the estrogen-induced pS2 mRNA. Potential aromatase inhibitors can be identified by a dose-dependent reduction in the pS2 mRNA expression level after exposure to testosterone and the test compound. Using this assay, we have investigated several compounds, including synthetic chemicals and phytoestrogens, for aromatase inhibition. The phytoestrogens, except genistein, were aromatase inhibitors at low concentrations (< 1 micro M) but estrogenic at higher concentrations (greater than or equal to 1 micro M), resulting in U-shaped dose-response curves. None of the tested synthetic chemicals were aromatase inhibitors. The low-dose aromatase inhibition distinguished phytoestrogens from other estrogenic compounds and may partly explain reports about antiestrogenic properties of phytoestrogens. Aromatase inhibition may play an important role in the protective effects of phytoestrogens against breast cancer.
authors.affiliation: Department of Growth and Reproduction, Rigshospitalet, Copenhagen, Denmark. Kristian.Almstrup@biobase.dk
authors.forename: Kristian
authors.initials: K
authors.lastname: Almstrup
authors.forename: Mariana F
authors.initials: MF
authors.lastname: Fernández
authors.forename: Jørgen H
authors.initials: JH
authors.lastname: Petersen
authors.forename: Nicolas
authors.initials: N
authors.lastname: Olea
authors.forename: Niels E
authors.initials: NE
authors.lastname: Skakkebaek
authors.forename: Henrik
authors.initials: H
authors.lastname: Leffers
mesh_terms: D047072:Aromatase Inhibitors; D001681:Biological Assay / Q000379:methods; D001943:Breast Neoplasms / Q000473:pathology* / Q000517:prevention & control; D018929:Cell Culture Techniques; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D005260:Female; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D047072:Aromatase Inhibitors; D004791:Enzyme Inhibitors; D004968:Estrogens, Non-Steroidal; D010936:Plant Extracts