[Lipase-catalyzed enantioselective ammonolysis of racemic phenylglycine methyl ester in organic solvent].
A novel reaction-enzymatic ammonolysis discovered in the mid of 1990s has been demonstrated to be a very promising alternative in the preparation of optically pure compounds. The effects of organic solvent, initial water activity, temperature and additives on lipase Novozym435-catalyzed enantioselective ammonolysis of racemic phenylglycine methyl ester were investigated systematically in this paper. Enzymatic reaction of ammonolysis showed higher activity and enantioselectivity than the corresponding reaction of hydrolysis and alcoholysis.
Du
Wei
W
Biotechnology Department, South China University of Technology, Guangzhou 510640, China. crystal-du@263.net
Zong
Min-Hua
MH
Guo
Yong
Y
He
Jun
J
Zhang
Yuan-Yuan
YY
Xie
Zhao-Lin
ZL
Lou
Wen-Yong
WY
D000438:Alcohols; D000641:Ammonia; D002384:Catalysis; D004126:Dimethylformamide / Q000494:pharmacology; D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism*; D006601:Hexoses / Q000494:pharmacology; D006868:Hydrolysis; D008049:Lipase / Q000187:drug effects / Q000378:metabolism*; D009930:Organic Chemicals; D012997:Solvents; D013501:Surface-Active Agents / Q000494:pharmacology; D013696:Temperature; D014867:Water
D000438:Alcohols; D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins; D006601:Hexoses; D009930:Organic Chemicals; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water; C018665:sorbitan monooleate; C033393:methyl phenylglycine; D000641:Ammonia; D004126:Dimethylformamide; C093667:Novozyme 435; D008049:Lipase; D005998:Glycine