Genetic analysis of rabies virus isolates in the Philippines.
To determine the genetic characteristics of the rabies virus in the Philippines, 59 rabies virus isolates were obtained from domestic rabid dogs and their partial nucleotide sequences of nucleoprotein (N) gene were compared. Based on comparison with reported sequences, phylogenetic analysis revealed that all isolates from the Philippines had close genetic relations and formed two subgroups. The Philippines isolates belonged to a different lineage from other Asian isolates but were closer to them than to terrestrial isolates and laboratory strains. Several specific nucleotide and amino acid substitutions were observed among the Philippines isolates. Our results suggest that rabies viruses in the Philippines might have a characteristic evolution.
Nishizono
Akira
A
Department of Microbiology, Oita Medical University, Japan. a24zono@oita-med.ac.jp
Mannen
Kazuaki
K
Elio-Villa
Luningning P
LP
Tanaka
Seiichi
S
Li
Kang-sheng
KS
Mifune
Kumato
K
Arca
Benito F
BF
Cabanban
Arturo
A
Martinez
Benjamin
B
Rodriguez
Arlito
A
Atienza
Victor C
VC
Camba
Rolando
R
Resontoc
Nilo
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004283:Dog Diseases / Q000453:epidemiology / Q000821:virology*; D004285:Dogs; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics; D010679:Philippines / Q000453:epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D011818:Rabies / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D011820:Rabies virus / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D019590:Nucleocapsid Proteins; D012367:RNA, Viral