Mutations in the p53 gene in acute myeloid leukemia patients correlate with poor prognosis.
Inactivation of tumor suppressor genes, whose products exert an inhibitory influence on cell cycle progression, can lead to neoplastic transformation. In acute myeloid leukemia (AML), the frequency of p53 gene mutations ranges from 4 to 15% in populations from USA and Europe. In an attempt to investigate the frequency of point mutations in the p53 gene in AML Brazilian patients, DNA samples of 35 patients were studied using PCR-SSCP techniques, screening exons 4-10. Mutations were identified in bone marrow DNA in 5 of the 35 AML patients (14.3%), a frequency similar to those reported for Northern American and European populations. The overall survival of patients with mutations in the p53 gene was significantly shorter than for patients without mutations.
Melo
Mônica B
MB
Department of Clinical Medicine--Hemocentro, School of Medical Sciences, State University of Campinas (UNICAMP), Campinas, S.P., Brazil.
Ahmad
Nilofer N
NN
Lima
Carmen S P
CS
Pagnano
Katia B B
KB
Bordin
Silvana
S
Lorand-Metze
Irene
I
SaAd
Sara T O
ST
Costa
Fernando F
FF
D000208:Acute Disease; D001853:Bone Marrow; D001938:Brazil / Q000453:epidemiology; D004252:DNA Mutational Analysis; D005091:Exons; D005787:Gene Frequency; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007951:Leukemia, Myeloid / Q000453:epidemiology / Q000235:genetics* / Q000401:mortality; D009154:Mutation / Q000235:genetics* / Q000502:physiology; D011379:Prognosis; D016019:Survival Analysis