title: Temporal profiles of extracellular nitric oxide metabolites following aneurysmal subarachnoid hemorrhage.
abstract: The temporal profile of nitric oxide metabolite concentrations i.e. nitrite and nitrate (NOx) was investigated in brain parenchyma of patients following aneurysmal subarachnoid hemorrhage (SAH). In a subset of ten patients (7F/3M, age: 47 +/- 14 yrs) included in a prospective clinical trial on neurochemical intensive-care monitoring, microdialysis (MD) probes (CMA70, Sweden) were implanted at time of aneurysm surgery. Samples from patients clipped electively (n = 3) were considered "normal" in regard to SAH patients (n = 7). MD was performed for 162 +/- 63 hrs. NOx was measured off-line using a highly sensitive, fluorometric assay (2-3-diaminonaphtalene, DAN). NOx concentrations determined from electively operated patients averaged 36.7 +/- 9.6 microM (n = 59, pooled data). Regardless of the development of delayed ischemic neurological deficits (DIND), SAH patients showed a specific temporal profile of NOx consisting of an initial peak followed by an exponential decay. In detail, NOx decreased from initial values of 46.2 +/- 34.8 microM to 23.5 +/- 9.0 microM on day 6-7 after SAH (p < 0.05). Following SAH extracellular concentrations of NO metabolites decrease over time. This is in agreement with hypothetical NO scavenging by products of hemolysis. However, subsequent development of DIND cannot be explained by a lack of vasodilatory NO alone.
authors.affiliation: Department of Neurosurgery, Charité-Humboldt University Berlin, Berlin, Germany.
authors.forename: O W
authors.initials: OW
authors.lastname: Sakowitz
authors.forename: S
authors.initials: S
authors.lastname: Wolfrum
authors.forename: A S
authors.initials: AS
authors.lastname: Sarrafzadeh
authors.forename: J F
authors.initials: JF
authors.lastname: Stover
authors.forename: W R
authors.initials: WR
authors.lastname: Lanksch
authors.forename: A W
authors.initials: AW
authors.lastname: Unterberg
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis; D001783:Blood Flow Velocity; D002560:Cerebrovascular Circulation / Q000502:physiology; D005260:Female; D006801:Humans; D002532:Intracranial Aneurysm / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D017551:Microdialysis / Q000379:methods; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism*; D013345:Subarachnoid Hemorrhage / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology; D013997:Time Factors
chemical_list: D015415:Biomarkers; D009569:Nitric Oxide