title: Functional diversity and mutational analysis of Agrobacterium 6B oncoproteins.
abstract: Many Agrobacterium T-DNA genes belong to a diverse family of T-DNA genes, the rolB family. These genes cause various growth abnormalities but their modes of action remain largely unknown. So far, none of the RolB-like proteins has been subjected to mutational analysis. The RolB-like oncoprotein 6B, which induces tumours on species such as Nicotiana glauca and Kalanchoe tubiflora, was chosen to investigate the role of the most conserved amino acid residues within the RolB family. We first determined which of the natural 6B variants had the strongest oncogenic activity; to this end, six 6b coding sequences (A- 6b, AB- 6b, C- 6b, CG- 6b, S- 6b and T- 6b) were placed under the control of the strong constitutive 2x35S promoter and compared for tumour induction on N. glauca, N. tabacum and K. daigremontiana. Oncogenicity increased in the order C- 6b/CG- 6b, A- 6b/AB- 6b, and S- 6b/T- 6b. The most conserved amino acid residues in the strongly oncogenic T-6B protein were mutated and shown to be required for oncogenicity and accumulation of the T-6B protein in planta but not in bacteria. Hybrids between T-6B and the weakly oncogenic A-6B protein revealed an additional oncogenic determinant required for the formation of large tumours.
authors.affiliation: Department of Cell Biology, Plant Molecular Biology Institute of the C. N. R. S., Rue du Général Zimmer 12, Strasbourg 67084, France.
authors.forename: A
authors.initials: A
authors.lastname: Helfer
authors.forename: S
authors.initials: S
authors.lastname: Pien
authors.forename: L
authors.initials: L
authors.lastname: Otten
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D004252:DNA Mutational Analysis; D014644:Genetic Variation; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D015513:Oncogene Proteins / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D012231:Rhizobium / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment; D014026:Nicotiana / Q000502:physiology; D001617:beta-Glucosidase / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D015513:Oncogene Proteins; C092241:RolB protein, Agrobacterium rhizogenes; D001617:beta-Glucosidase