Molecular cloning and characterization of a novel beta-1,3-glucanase gene from rice.
Beta-1,3-glucanases are referred to as pathogenesis-related proteins and they are also involved in several developmental processes. We isolated a cDNA for beta-1,3-glucanase from rice anther and named it Oryza sativa glucanase 1 (Osg1). Phylogenetic analysis showed that Osg1 belonged to monocotyledonous endo-beta-1,3-glucanase subgroup A. RT-PCR analysis revealed that Osg1 transcripts were present in leaves, roots, and anthers.
Yamaguchi
Tomoya
T
Plant Physiology Laboratory, National Agricultural Research Center for Tohoku Region, Morioka, Japan.
Nakayama
Katsuhiro
K
Hayashi
Takami
T
Tanaka
Yoshiyuki
Y
Koike
Setsuo
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D043326:Glucan 1,3-beta-Glucosidase; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001617:beta-Glucosidase / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase