Establishment of a novel enzyme-linked immunosorbent assay for Thy-1; quantitative assessment of neuronal degeneration.
In the central nervous system (CNS), Thy-1 is expressed predominantly on neurons and serves as a specific marker for neurons. In the present study, we established a two-site enzyme-linked immunosorbent assay (ELISA) that detects trace amounts of Thy-1 protein. Recombinant Thy-1 protein expressed in Escherichia coli was purified and used as a standard. Of the regions of the nervous system examined, the highest Thy-1 concentration was found in the striatum followed by the hippocampus, neocortex, cerebellum, spinal cord, retina and optic nerve. We found that injection of a neurotoxin, N-methyl-D-aspartate, into the vitreous cavity reduced the Thy-1 level in the retina. Thy-1 ELISA will be useful for quantitative assessment of neurodegeneration in the CNS.
Seki
Masaaki
M
Department of Molecular Neurobiology, Brain Research Institute, Niigata University, Asahimachi 1, 951-8585 Niigata, Japan.
Nawa
Hiroyuki
H
Morioka
Tetsuo
T
Fukuchi
Takeo
T
Oite
Takashi
T
Abe
Haruki
H
Takei
Nobuyuki
N
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D004926:Escherichia coli; D019579:Neocortex / Q000737:chemistry / Q000378:metabolism; D009410:Nerve Degeneration / Q000378:metabolism*; D051381:Rats; D012160:Retina / Q000737:chemistry / Q000378:metabolism; D018800:Thy-1 Antigens / Q000032:analysis* / Q000096:biosynthesis
D018800:Thy-1 Antigens