A conditionally fertile coi1 allele indicates cross-talk between plant hormone signalling pathways in Arabidopsis thaliana seeds and young seedlings.
Jasmonates (JAs) regulate Arabidopsis thaliana (L.) Heynh. wound and defense responses, pollen development, and stress-related growth inhibition. Significantly, each of these responses requires COI1, an F-box protein. We fused firefly luciferase as a reporter to the JA-responsive promoter for the vegetative storage protein gene (VSP) and used this to screen for mutants that failed to express luciferase in the presence of JA, isolating a mutant designated coi1-16. Comparisons with coi1-1 and jar1-1 plants indicated that coi1-16 was only slightly more sensitive to JA than coi1-1 plants. However, whilst coi1-16 plants failed to produce viable pollen at 22 degrees C, they were fertile at 16 degrees C. Therefore, unlike the other coi1 mutants, coi1-16 could be maintained as a pure line and did not require selection. We have used coi1-16 seeds to define novel interactions between JA and other hormone signalling pathways in seed germination and in the development of young seedlings.
Ellis
Christine
C
School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK.
Turner
John G
JG
D000040:Abscisic Acid / Q000494:pharmacology; D000085:Acetates / Q000494:pharmacology; D000483:Alleles; D019943:Amino Acid Substitution; D000818:Animals; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology; D003624:Darkness; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D005030:Ethylenes / Q000494:pharmacology; D005298:Fertility / Q000187:drug effects / Q000235:genetics; D018546:Hypocotyl / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D007930:Leucine / Q000235:genetics; D009154:Mutation; D020125:Mutation, Missense; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins; D010649:Phenylalanine / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D018514:Plant Structures / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D030821:Plants, Genetically Modified; D011058:Pollen / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D011401:Promoter Regions, Genetic / Q000235:genetics; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology; D013314:Stress, Mechanical; D013696:Temperature; D013997:Time Factors; D010453:Peptide Synthases
D000040:Abscisic Acid; D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D010450:Endopeptidases; D005030:Ethylenes; D007930:Leucine; D009713:Nucleotidyltransferases; D054883:Oxylipins; D010649:Phenylalanine; D010937:Plant Growth Regulators; D012965:Sodium Chloride; C112279:COI1 protein, Arabidopsis; C437085:THI1 protein, Arabidopsis; C463539:VSP2 protein, Arabidopsis; C072239:methyl jasmonate; C036216:ethylene; C441479:VSP1 protein, Arabidopsis; C412831:AT2G46370 protein, Arabidopsis; D010453:Peptide Synthases