title: Distribution of beta-lactamases in Acinetobacter baumannii clinical isolates and the effect of Syn 2190 (AmpC inhibitor) on the MICs of different beta-lactam antibiotics.
abstract: The distribution of beta-lactamases in a group of 20 epidemiologically well defined Acinetobacter baumannii clinical isolates and the in vitro activity of Syn 2190, a novel beta-lactamase AmpC inhibitor, were determined. Twenty-five per cent of the strains carried and expressed a TEM-type beta-lactamase, whereas 35% had an OXA-type beta-lactamase. In nine out of 11 (82%) ceftazidime-resistant and four out of 13 (30.7%) cefepime-resistant strains, the MIC of these beta-lactam antibiotics decreased when determined in the presence of Syn 2190. Thus, our results suggest that in a high percentage of A. baumannii clinical isolates the increased production of AmpC, in combination or not with other resistance mechanisms, contributes to the resistance pattern in A. baumannii to beta-lactams.
authors.affiliation: Servei de Microbiologia, Institut Clínic Infeccions i Immunologia, Hospital Clínic-IDIBAPS, University of Barcelona, Villarroel 170, 08036 Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Danes
authors.forename: Margarita M
authors.initials: MM
authors.lastname: Navia
authors.forename: Joaquim
authors.initials: J
authors.lastname: Ruiz
authors.forename: Francesc
authors.initials: F
authors.lastname: Marco
authors.forename: Angels
authors.initials: A
authors.lastname: Jurado
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Jimenez de Anta
authors.forename: Jordi
authors.initials: J
authors.lastname: Vila
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000706:statistics & numerical data; D008997:Monobactams / Q000494:pharmacology*; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D008997:Monobactams; C120996:Syn 2190; D065093:beta-Lactamase Inhibitors; C048795:AmpC beta-lactamases; D001618:beta-Lactamases