Developmental changes in the expression of enterocytic and hepatic cytochromes P4501A in rat.
1. The development of CYP1A enzymes was studied in enterocytic and hepatic microsomes from 1-day-old to adult male and female rats. Microsomes were prepared by calcium precipitation. Enzyme expression was determined by Western blotting using a polyclonal CYP1A1 antibody. 2. The developmental expression of CYP1A in enterocytic and hepatic microsomes was similar for males and females. 3. Enterocytic CYP1A (CYP1A1) showed a sharp increase at weaning, plateauing at adult levels by 60 days. 4. Hepatic CYP1A (mostly CYP1A2) increased sharply just before weaning. However, in contrast to the enterocytic enzyme, there was a 4-fold decrease in enzyme expression down to adult levels by day 60.
Johnson
T N
TN
University of Sheffield, Academic Unit of Molecular Pharmacology and Pharmacogenetics, Division of Clinical Sciences, Royal Hallamshire Hospital, Sheffield S10 2JF, UK. t.n.johnson@sheffield.ac.uk
Tanner
M S
MS
Tucker
G T
GT
D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D019388:Cytochrome P-450 CYP1A2 / Q000378:metabolism*; D020895:Enterocytes / Q000201:enzymology*; D005260:Female; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000201:enzymology / Q000254:growth & development; D008099:Liver / Q000254:growth & development; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar
D019363:Cytochrome P-450 CYP1A1; D019388:Cytochrome P-450 CYP1A2