Rapid recycle (13)C',(15)N and (13)C,(13)C' heteronuclear and homonuclear multiple quantum coherence detection for resonance assignments in paramagnetic proteins: example of Ni(2+)-containing acireductone dioxygenase.
NMR resonance assignments in the vicinity of paramagnetic metals in proteins are often difficult or impossible to make using conventional 1H detected 2-D and 3-D methods due to paramagnetic line broadening. The applicability of 13Calpha{13C'} and 13C'{15N} multiple quantum coherence methods for residue-specific assignments of resonances near paramagnetic centers is described, using the Ni2+-containing enzyme acireductone dioxygenase as an example.
Kostic
Milka
M
Department of Chemistry, Brandeis University, 415 South Street, Waltham, Massachusetts 02454, USA.
Pochapsky
Susan Sondej
SS
Pochapsky
Thomas C
TC
D049308:Dioxygenases; D007711:Klebsiella pneumoniae / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D010105:Oxygenases / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D010105:Oxygenases; D049308:Dioxygenases; C083734:aci-reductone oxidase (CO-forming)