The serine proteinase thrombin promotes migration of human renal carcinoma cells by a PKA-dependent mechanism.
In this study, we demonstrated that thrombin activates protein kinase C (PKC), mitogen activated protein kinases (MAP kinases), transcription factor nuclear factor-kappa B (NF-kappa B), and cAMP-dependent protein kinase (PKA) in the human renal carcinoma cell line A-498. In addition, it enhanced the migratory capacity, but had no effect on the proliferation of A-498 cells. The effect of thrombin on migration could be blocked by the PKA inhibitor H-89 but was not influenced by inhibition of PKC, MAP kinases or NF-kappa B. We concluded, that thrombin acts as a regulator on human A-498 renal carcinoma cell migration including PKA.
Kaufmann
R
R
Department of General and Visceral Surgery, Medical Faculty at the Friedrich Schiller University Jena, Bachstr. 18, 07740 Jena, Germany. roland.kaufmann@med.uni-jena.de
Junker
U
U
Junker
K
K
Nuske
K
K
Ranke
C
C
Zieger
M
M
Scheele
J
J
D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism; D002633:Chemotaxis / Q000187:drug effects; D017868:Cyclic AMP-Dependent Protein Kinases / Q000502:physiology*; D004789:Enzyme Activation; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011493:Protein Kinase C / Q000502:physiology; D013917:Thrombin / Q000494:pharmacology*; D051996:Transcription Factor RelA; D014407:Tumor Cells, Cultured
D016328:NF-kappa B; D051996:Transcription Factor RelA; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D020928:Mitogen-Activated Protein Kinases; D013917:Thrombin