Comparative analysis of the roles of simian immunodeficiency and bovine leukemia virus matrix proteins in Gag assembly in insect cells.
The role of the matrix (MA) domain of simian immunodeficiency virus (SIV) and bovine leukaemia virus (BLV) Gag in the assembly of virus-like particles (VLP) in insect cells has been investigated. Wild-type SIV and BLV Gag assembled to form discrete VLP structures typical of many retroviruses analysed by similar systems. When amino acids predicated by the three-dimensional structure to be at the interface of SIV MA monomers were deleted, VLP assembly was abolished consistent with a role for MA multimerization in assembly. When amino acids predicted to be in the analogous positions in BLV MA were mutated, however, VLP assembly was not affected. These data indicate that the models of assembly derived from one model retrovirus may not necessarily apply to more distantly related viruses despite the structural similarity present in equivalent Gag domains.
Kakker
Naresh K
NK
Department of Biochemistry, University of Oxford, South Parks Road, Oxford, United Kingdom.
Mikhailov
Michael V
MV
Jones
Ian M
IM
Roy
Polly
P
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002478:Cells, Cultured; D015683:Gene Products, gag / Q000096:biosynthesis / Q000235:genetics*; D005822:Genetic Vectors; D007313:Insecta; D001909:Leukemia Virus, Bovine / Q000737:chemistry / Q000502:physiology*; D009154:Mutation; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic; D012756:Sheep; D015302:Simian Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D019065:Virus Assembly
D015683:Gene Products, gag; D014763:Viral Matrix Proteins