title: The effect of transforming growth factor-beta1, released from a bioabsorbable self-reinforced polylactide pin, on a bone defect.
abstract: Transforming growth factor-beta 1 (TGF-beta1)is a polypeptide growth factor which has been shown to increase bone formation in experimental studies. In this study it was combined to a bioabsorbable self-reinforced poly-LD-lactic acid fracture fixation pin. To assess the effect of TGF-beta1 on the healing of a bone defect, the pins were implanted in the rat distal femur next to a bone defect filled with a viscose cellulose sponge. The pins used in the study group (13 rats) contained 50 microg of TGF-beta1, whereas in the control group of nine rats an identical pin without the growth factor was used. In the histologic examination at 1, 3 and 6 weeks no difference was detected in the amount of bone inside the viscose cellulose sponge between the rats treated with TGF-beta1 and those with no added growth factor. At 3 weeks there was more fibroblast-rich mesenchymal tissue inside the viscose cellulose sponge in the rats treated with TGF-beta1. In the radiographic examination at 3 weeks there was an increase in the amount of new periosteal bone on the bone defect in the TGF-beta1-treated rats.
authors.affiliation: Department of Surgery, Helsinki University Hospital, Finland. laura.tielinen@hus.fi
authors.forename: Laura
authors.initials: L
authors.lastname: Tieline
authors.forename: Pauli
authors.initials: P
authors.lastname: Puolakkainen
authors.forename: Timo
authors.initials: T
authors.lastname: Pohjonen
authors.forename: Jorma
authors.initials: J
authors.lastname: Rautavuori
authors.forename: Pertti
authors.initials: P
authors.lastname: Törmälä
authors.forename: Pentti
authors.initials: P
authors.lastname: Rokkanen
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials; D001858:Bone Nails; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000187:drug effects; D002482:Cellulose / Q000737:chemistry; D020099:Coated Materials, Biocompatible; D005264:Femoral Fractures / Q000000981:diagnostic imaging / Q000628:therapy*; D005593:Fracture Fixation, Internal / Q000295:instrumentation*; D017102:Fracture Healing / Q000187:drug effects*; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D011091:Polyesters; D011108:Polymers; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D019344:Lactic Acid; C033616:poly(lactide); D002482:Cellulose