A serial analysis of gene expression in sun-damaged human skin.
To study the phenotypic changes in human skin associated with repeated sun exposure at the transcription level, we have undertaken a comparative serial analysis of gene expression of sun-damaged preauricular skin and sun-protected postauricular skin as well as sun-protected epidermis. Serial analyses of gene expression libraries, containing multiple mRNA-derived tag recombinants, were made to poly(A+)RNA isolated from human postauricular skin and preauricular skin, as well as epidermal nick biopsy samples. 5330 mRNA-derived cDNA tags from the postauricular serial analysis of gene expression library were sequenced and these tag sequences were compared to cDNA sequences identified from 5105 tags analyzed from a preauricular serial analysis of gene expression library. Of the total of 4742 different tags represented in both libraries we found 34 tags with at least a 4-fold difference of tag abundance between the libraries. Among the mRNAs with altered steady-state(1) levels in sun-damaged skin, we detected those encoding keratin 1, macrophage inhibitory factor, and calmodulin-like skin protein. In addition, a comparison of cDNA sequences identified in the serial analysis of gene expression libraries obtained from the epidermal biopsy samples (5257 cDNA tags) and from both full-thickness skin samples indicated that many genes with altered steady-state transcript levels upon sun exposure were expressed in epidermal keratinocytes. These results suggest a major role for the epidermis in the pathomechanism of largely dermal changes in chronically sun-exposed skin.
Urschitz
Johann
J
The Pacific Biomedical Research Center, University of Hawaii, 1993 East-West Road, Honolulu, HI 96822, U.S.A.
Iobst
Susanne
S
Urban
Zsolt
Z
Granda
Chia
C
Souza
Kathleen A
KA
Lupp
Claudia
C
Schilling
Kurt
K
Scott
Ian
I
Csiszar
Katalin
K
Boyd
Charles D
CD
D000328:Adult; D001706:Biopsy; D015152:Blotting, Northern; D004431:Ear, External; D004548:Elasticity; D004817:Epidermis / Q000473:pathology / Q000502:physiology* / Q000528:radiation effects*; D005260:Female; D015870:Gene Expression / Q000528:radiation effects; D020869:Gene Expression Profiling; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D015595:Skin Aging / Q000235:genetics; D013472:Sunlight / Q000009:adverse effects*
D012333:RNA, Messenger