Adrenomedullin over-expression in the caudate-putamen of the adult rat brain after ischaemia-reperfusion injury.
The expression of adrenomedullin (AM) was studied in the caudate-putamen of the adult rat brain using a global cerebral ischaemia model. The animals were subjected to 30 min of glucose and oxygen deprivation, and the brains were collected after 0, 2, 4, 6, 8 and 10 h of reperfusion. Coronal sections of the caudate-putamen were studied by immunocytochemistry using a specific polyclonal antibody against AM and examined by light microscopy. Under these experimental conditions AM immunoreactivity increased in the wall of the blood vessels and in three different types of neurons distributed throughout the caudate-putamen. Our findings suggest that the over-expression of AM and its changes in its intracellular location might be involved in the neuronal responses to brain ischaemia with a possible neuroprotector role.
Encinas
Juan-Manuel
JM
Department of Neuroanatomy and Cell Biology, Instituto de Neurobiología Ramón y Cajal, CSIC, Avenida Doctor del Arce 37, 28002 Madrid, Spain.
Serrano
Julia
J
Alonso
David
D
Fernández
Ana-Patricia
AP
Rodrigo
José
J
D053607:Adrenomedullin; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D002421:Caudate Nucleus / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010452:Peptide Biosynthesis / Q000502:physiology*; D010455:Peptides / Q000378:metabolism*; D011699:Putamen / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*
D010455:Peptides; D053607:Adrenomedullin