Gene expression of bone matrix proteins in a calcified tissue appeared in subcutaneously transplanted rat dental pulp.
Dental pulp self-mineralizes or induces calcified tissue formation, when it is subcutaneously transplanted. This study aims to clarify the nature and process of this tissue calcification by using histochemical techniques and in situ hybridization methods. The dental pulps of incisors from Sprague-Dawley strain rats were homogeneously transplanted into the subcutaneous tissue of the head of another rat. On the 1st day of transplantation, the mRNAs of type I collagen (COL I) and bone sialoprotein (BSP) were expressed in the transplant. On the 5th to 7th days after transplantation, von Kossa-positive, calcified tissues appeared in the transplanted tissues. The mRNAs of COL I, BSP and osteopontin (OPN) were expressed with the same distributions as those of the von Kossa-positive areas, but those of type II collagen (COL II) and bone morphogenic protein 2 (BMP-2) were not expressed in the transplant. When the cellular incorporation of BrdU was examined after its subcutaneous injection into the animals, positive cells were present in the von Kossa-positive calcified foci as well as in their surrounding areas. These results indicate that the transplanted dental pulp possesses the ability to induce cell proliferation resulting in the synthesis of several bone matrix proteins and the formation of calcified tissue. This may give us an insight into the nature and processes of pulp obliteration, which occurs in a human tooth following an episode of trauma.
Yamazoe
Tomohiro
T
Department of Orofacial Development and Function, Graduate School, Tokyo Medical and Dental University, Japan.
Aoki
Kazuhiro
K
Simokawa
Hitoyata
H
Ohya
Keiichi
K
Takagi
Yuzo
Y
D000818:Animals; D000963:Antimetabolites; D001011:Aorta / Q000378:metabolism / Q000637:transplantation; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000032:analysis / Q000235:genetics; D001973:Bromodeoxyuridine; D002455:Cell Division; D024042:Collagen Type I / Q000032:analysis* / Q000235:genetics; D024043:Collagen Type II / Q000032:analysis / Q000235:genetics; D003782:Dental Pulp / Q000378:metabolism / Q000637:transplantation*; D003784:Dental Pulp Calcification / Q000378:metabolism*; D062109:Dermatologic Surgical Procedures; D015870:Gene Expression; D006651:Histocytochemistry; D017403:In Situ Hybridization; D058574:Integrin-Binding Sialoprotein; D009061:Mouth Mucosa / Q000378:metabolism / Q000637:transplantation; D053495:Osteopontin; D010750:Phosphoproteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000032:analysis* / Q000235:genetics; D016622:Silver Staining; D013710:Tendons / Q000378:metabolism / Q000637:transplantation; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000235:genetics
D000963:Antimetabolites; C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D024042:Collagen Type I; D024043:Collagen Type II; C546871:IBSP protein, human; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D010750:Phosphoproteins; D012333:RNA, Messenger; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D016212:Transforming Growth Factor beta; D053495:Osteopontin; D001973:Bromodeoxyuridine