title: [Dual-color FISH study on myelodysplastic syndrome with 1;7 translocation].
abstract: OBJECTIVE
  To study the myelodysplastic syndrome(MDS) with 1;7 translocation in five cases and to determine further the constitution and origin of centromere of the derivative chromosome resulting from 1;7 translocation.
  
  
  METHODS
  Bone marrow chromosome preparation of five cases was made using direct method or short- term culture. Karyotypic analysis was carried out by R-banding technique. Dual-color fluorescence in situ hybridization(FISH) using Spectrum Red and Spectrum Green directly labeled chromosome 1-specific a-satellite DNA probe(red) and chromosome 7-specific a-satellite DNA probe(green) was performed in three patients of them.
  
  
  RESULTS
  All of the five cases had 1;7 translocation. The centromere of the derivative chromosome 7p/1q was constituted with red and green signals in three of them.
  
  
  CONCLUSIONS
  The result of dual-color FISH confirms that the centromere of the derivative chromosome resulting from 1;7 translocation originated from both centromeres of chromosome 1 and chromosome 7.
authors.affiliation: First Affiliated Hospital of Suzhou University, Jiangsu Institute of Hematology, Suzhou, Jiangsu, 215006 P.R.China. uujihsmc@public1.sz.js.cn
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Shen
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Xue
authors.forename: Jianyong
authors.initials: J
authors.lastname: Li
authors.forename: Jinlan
authors.initials: J
authors.lastname: Pan
authors.forename: Yafang
authors.initials: Y
authors.lastname: Wu
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.forename: Dingwei
authors.initials: D
authors.lastname: Lu
mesh_terms: D000328:Adult; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D008297:Male; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D014178:Translocation, Genetic