title: Quantitative evaluation of angiogenesis in laryngeal cancer by digital image measurement of the vessel density.
abstract: OBJECTIVE
  the aim of our study was determination of the relationship between angiogenesis and clinical as well as histological features in laryngeal cancer.
  
  
  METHODS
  we used two different methods of estimation of the amount of microvessels in the series of 55 cases, e.g. classical count of endothelial cells group (h-MVD) and digital image measurement of the vessel density (VD).
  
  
  RESULTS
  neither h-MVD nor VD correlated significantly with clinical features of the tumour. The results of VD examination correlated significantly with the existence of nodal metastases (P=0.02). The relationship between h-MVD and N status was on the statistical borderline (P=0.07). Multivariate analysis of Cox's proportional hazards model revealed that only N status correlated significantly with patients' survival.
  
  
  CONCLUSIONS
  our results indicate that measurements of angiogenesis in laryngeal cancer may be of some value in predicting N status in laryngeal cancer patients. This issue should be confirmed in prospective studies.
authors.affiliation: Department of Otolaryngology, Medical University of Wrocław, ul. Smoleńskiego 4, 51-607 Wrocław, Poland. krecicki@orl.am.wroc.pl
authors.forename: Tomasz
authors.initials: T
authors.lastname: Krecicki
authors.forename: Danuta
authors.initials: D
authors.lastname: Dus
authors.forename: Joanna
authors.initials: J
authors.lastname: Kozlak
authors.forename: Wojciech
authors.initials: W
authors.lastname: Tarnawski
authors.forename: Michal
authors.initials: M
authors.lastname: Jelen
authors.forename: Maria
authors.initials: M
authors.lastname: Zalesska-Krecicka
authors.forename: Teresa
authors.initials: T
authors.lastname: Szkudlarek
mesh_terms: D000368:Aged; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D007822:Laryngeal Neoplasms / Q000098:blood supply* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008833:Microcirculation / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000473:pathology*; D011379:Prognosis; D011446:Prospective Studies