Determination of mitochondrial reactive oxygen species: methodological aspects.
The generation of Reactive Oxygen Species (ROS) as by-products in mitochondria Electron Transport Chain (ETC) has long been admitted as the cost of aerobic energy metabolism with oxidative damages as consequence. The purpose of this methodological review is to present some of the most widespread methods of ROS generation and to underline the limitations as well as some problems, identified with some experiments as examples, in the interpretation of such results. There is now no doubt that besides their pejorative role, ROS are involved in a variety of cellular processes for the continuous adaptation of the cell to its environment. Because ROS metabolism is a complex area (low production, instability of species, efficient antioxidant defense system, several places of production...) bias, variances and limitations in ROS measurements must be recognized in order to avoid artefactual conclusions, and especially to improve our understanding of physiological and pathophysiological mechanisms of such phenomenon.
Batandier
Cécile
C
Laboratoire de Bioénergétique Fondamentale et Appliquée INSERM E0221 et Université J. Fourier, BP 53X, 38041 Grenoble Cedex, France.
Fontaine
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E
Kériel
Christiane
C
Leverve
X M
XM
D000704:Analysis of Variance; D000975:Antioxidants / Q000378:metabolism; D015982:Bias; D004579:Electron Transport; D005470:Fluorometry / Q000379:methods; D008928:Mitochondria / Q000378:metabolism*; D008954:Models, Biological; D018384:Oxidative Stress / Q000502:physiology; D017382:Reactive Oxygen Species / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods
D000975:Antioxidants; D017382:Reactive Oxygen Species