Ruscogenin glycoside (Lm-3) isolated from Liriope muscari inhibits lymphocyte adhesion to extracellular matrix.
We examined the effects of ruscogenin glycoside (Lm-3), isolated from Liriope muscari, on lymphocyte adhesion to extracellular matrix. Adhesion of Jurkat cells activated by anti-CD3 to type I collagen was inhibited by Lm-3 in a concentration- and time-dependent manner. Lm-3 also inhibited the cell attachment to fibronectin and laminin. However, the saponin did not influence anti-CD3-induced cell proliferation and Mn2+-induced adhesion. Protein kinase C activator, phorbol 12,13-dibutyrate, significantly enhanced, while its inhibitor, chlorpromazine, almost completely blocked, the adhesion of anti-CD3-activated Jurkat cells to collagen. Against phorbol 12,13-dibutyrate-activated Jurkat cells, Lm-3 treatment, either before or after activation, significantly inhibited the cell adhesion to collagen. Lm-3 also inhibited the adhesion activated by both anti-CD3 and phorbol 12,13-dibutyrate. Similar inhibition by Lm-3 of the phorbol 12,13-dibutyrate-induced adhesion to collagen was also observed in lymphocytes freshly isolated from mice with contact dermatitis. Furthermore, Lm-3 significantly decreased the leucocyte accumulation in an animal model of experimental pleurisy. These results suggest that the blockade of lymphocyte adhesion to extracellular matrix through interference with the protein kinase C pathway may be one of the mechanisms by which Lm-3 exerts anti-inflammatory activity.
Liu
Jianli
J
Department of Pharmacology for Chinese Materia Medica, China Pharmaceutical University, Nanjing.
Chen
Ting
T
Yu
Boyang
B
Xu
Qiang
Q
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix; D005260:Female; D006801:Humans; D007249:Inflammation; D019169:Jurkat Cells; D031424:Liriope Plant / Q000737:chemistry; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts; D010998:Pleurisy; D011493:Protein Kinase C / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology*; D013150:Spirostans / Q000494:pharmacology*
D010936:Plant Extracts; D012503:Saponins; D013150:Spirostans; C421631:ruscogenin-1-yl xylopyranosyl-1-3-(glucopyranosyl-1-2)fucopyranoside; D011493:Protein Kinase C