Involvement of hedgehog, wingless, and dpp in the initiation of proximodistal axis formation during the regeneration of insect legs, a verification of the modified boundary model.
To understand the mechanism of regeneration, many experiments have been carried out with hemimetabolous insects, since their nymphs possess the ability to regenerate amputated legs. We first succeeded in observing expression patterns of hedgehog, wingless (wg), and decapentaplegic (dpp) during leg regeneration of the cricket Gryllus bimaculatus. The observed expression patterns were essentially consistent with the predictions derived from the boundary model modified by Campbell and Tomlinson (CTBM). Thus, we concluded that the formation of the proximodistal axis of a regenerating leg is triggered at a site where ventral wg-expressing cells abut dorsal dpp-expressing cells in the anteroposterior (A/P) boundary, as postulated in the CTBM.
Mito
Taro
T
Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, 2-1 Minami-Jyosanjima-cho, Tokushima City 770-8506, Japan.
Inoue
Yoshiko
Y
Kimura
Shinsuke
S
Miyawaki
Katsuyuki
K
Niwa
Nao
N
Shinmyo
Yohei
Y
Ohuchi
Hideyo
H
Noji
Sumihare
S
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division; D029721:Drosophila Proteins / Q000502:physiology*; D005121:Extremities / Q000033:anatomy & histology / Q000502:physiology*; D006135:Gryllidae / Q000502:physiology*; D053823:Hedgehog Proteins; D008953:Models, Anatomic; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D012038:Regeneration; D013997:Time Factors; D051155:Wnt1 Protein
D029721:Drosophila Proteins; D053823:Hedgehog Proteins; D011518:Proto-Oncogene Proteins; D051155:Wnt1 Protein; C064196:dpp protein, Drosophila; C074622:wg protein, Drosophila; C077245:hh protein, Drosophila; D001973:Bromodeoxyuridine