[Nested coamplification  polymerase chain reaction].
OBJECTIVE
To develop a simple and reliable method for intensifying the hybridization signals of gene chips.


METHODS
The authors added EDTA and another FAM-labeled probe to the normal PCR products, denatured the mixture by heat, and then let the mixture hybridize with the fastened probes on the chip.


RESULTS
With the use of EDTA and another FAM-labeled probe, the hybridization signals increased by 6 times or greater.


CONCLUSIONS
Adding EDTA and another probe to the normal PCR products is a simple and efficient method to intensify the hybridization signal of chips.
Xia
Qingjie
Q
Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041 P.R.China. szzhang@mcwcms.com
Zhang
Sizhong
S
Yang
Yuan
Y
Wang
Jun
J
Wang
Xiujie
X
Ma
Yongxin
Y
Sun
Yan
Y
D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004492:Edetic Acid / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results
D015342:DNA Probes; D005456:Fluorescent Dyes; D004492:Edetic Acid