title: Cloning of zebrafish T-box genes tbx15 and tbx18 and their expression during embryonic development.
abstract: Members of the T-box (tbx) gene family encode developmentally regulated transcription factors, several of which are implicated in human hereditary diseases. We have cloned the paralogous genes tbx15 and tbx18 in zebrafish and have characterised their expression in detail. tbx15 is expressed in paraxial head mesenchyme and its derivatives, the extraocular and jaw musculature and the posterior neurocranium. Further areas of tbx15 expression are in the anterior somitic mesoderm, in periocular mesenchyme and in the pectoral fin mesenchyme throughout larval development. Areas of strong tbx18 expression are found in the developing somitic and presomitic mesoderm, in the heart and in pectoral fin mesenchyme, as well as the ventral neuroectoderm and the developing palate. Both genes exhibit particular differences in expression compared to their murine orthologs.
authors.affiliation: MRC Intercellular Signalling Group, Centre for Developmental Genetics, Department of Biomedical Science, University of Sheffield School of Medicine, Firth Court, Western Bank, Sheffield S10 2TN, UK. gerrit.begemann@uni-konstanz.de
authors.forename: Gerrit
authors.initials: G
authors.lastname: Begemann
authors.forename: Yann
authors.initials: Y
authors.lastname: Gibert
authors.forename: Axel
authors.initials: A
authors.lastname: Meyer
authors.forename: Phillip W
authors.initials: PW
authors.lastname: Ingham
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology; D017403:In Situ Hybridization; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D020825:T-Box Domain Proteins / Q000096:biosynthesis / Q000235:genetics*; D013997:Time Factors; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins
chemical_list: D020825:T-Box Domain Proteins; C114093:TBX15 protein, mouse; C420983:Tbx18 protein, mouse; D014157:Transcription Factors; D029961:Zebrafish Proteins; C485249:tbx15 protein, zebrafish; C486011:tbx18 protein, zebrafish