Human polyoma virus BK DNA detection by nested PCR in renal transplant recipients.
Several studies report a correlation between the human polyomavirus BK (BKV) and interstitial nephritis in renal transplant recipients in whom immunosuppressive treatment is thought to allow or induce reactivation of the virus. Furthermore, it is described that nephropathy may result from the use of newly introduced immunosuppressive drugs. In the present study, we evaluated the presence of BKV DNA by nested-PCR (n-PCR) in urine and serum samples from 35 renal transplant patients related to the immunosuppressive regimens and renal function.
Bergallo
M
M
Department of Public Health and Microbiology, University of Turin, Italy.
Merlino
C
C
Bollero
C
C
Scutera
S
S
Sinesi
F
F
Cavallo
R
R
D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification*; D003404:Creatinine / Q000097:blood; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics / Q000652:urine*; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D007166:Immunosuppressive Agents / Q000009:adverse effects; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D009395:Nephritis, Interstitial / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D027601:Polyomavirus Infections / Q000097:blood / Q000150:complications* / Q000652:urine; D014412:Tumor Virus Infections / Q000097:blood / Q000150:complications* / Q000652:urine; D017201:Virus Shedding
D004279:DNA, Viral; D007166:Immunosuppressive Agents; D003404:Creatinine