title: [The 23-kDa polypeptide from Common Frog lens: isolation, characteristics and cellular localization].
abstract: The major water-soluble polypeptide with molecular weight of approximately 23 kDa (the 23-kDa polypeptide) was identified in the lens of common frog Rana temporaria L. According to the gel filtration data, the peptide is a part of an oligomeric protein with molecular weight of more than 300 kDa (alpha-crystallin fraction). A highly pure fraction of the 23-kDa polypeptide was isolated by two-step ion-exchange chromatography and SDS electrophoresis and the specific antibodies were obtained. Immunohistochemistry showed the presence of the 23-kDa polypeptide in the cytoplasm of lens epithelial cells (including its central region) and in the zones neighbouring the plasma membranes in cortical fibers. The 23-kDa polypeptide was not found in the lens central zone (nucleus). It was also present in the retina (in the cells of inner nuclear layers), but not in the other tissues and organs of adult frog. Immunochemical analysis showed that the 23-kDa polypeptide was different from all known crystallins of frogs and other animals (bull, mouse, rat, and chicken). The nature of the 23-kDa polypeptide and the relation of its expression with lens cell differentiation are discussed.
authors.affiliation: Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Simirskiĭ
authors.forename: K S
authors.initials: KS
authors.lastname: Aleĭnikova
mesh_terms: D000818:Animals; D003459:Crystallins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009928:Organ Specificity; D011896:Rana temporaria; D051381:Rats; D013045:Species Specificity
chemical_list: D003459:Crystallins