Brain HSP70 mRNA expression is linked with plasma cortisol levels in goldfish (Carassius auratus) exposed to a potential predator.
We previously found that when goldfish were exposed to a potential predator, bluegills, the goldfish experienced an increase in HSP70 mRNA expression in the brains and increased plasma cortisol levels. In the present study, we examined the potential causative relationship between HSP70 mRNA expression and plasma cortisol levels. Cortisol agonists (corticotropin releasing factor and cortisol) and antagonists (metyrapone and betamethasone) were used to modulate plasma cortisol levels. HSP70 mRNA expression and plasma cortisol levels were analyzed by Northern blotting and ELISA, respectively. Goldfish treated with the cortisol agonists showed marked increases in plasma cortisol levels and also in brain HSP70 mRNA expression. When goldfish were exposed to bluegills, plasma cortisol levels increased and HSP70 mRNA expression was enhanced after 6 hr. However, pre-treatment with the cortisol antagonists 24 hr prior to the exposure inhibited the enhancement as well as the increase in plasma cortisol levels. These results suggest that plasma cortisol plays a key role in the enhancement of brain HSP70 mRNA expression in goldfish stressed by exposure to bluegills.
Kagawa
Nao
N
Department of Life Science, Faculty of Science and Engineering, Kinki University, Higashi-Osaka, Japan. nkagawa@msa.kindai.ac.jp
Mugiya
Yasuo
Y
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001623:Betamethasone / Q000494:pharmacology; D015152:Blotting, Northern; D001923:Brain Chemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D005938:Glucocorticoids / Q000494:pharmacology; D006054:Goldfish / Q000097:blood* / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006854:Hydrocortisone / Q000819:agonists / Q000037:antagonists & inhibitors / Q000097:blood*; D008797:Metyrapone / Q000494:pharmacology; D010473:Perciformes / Q000502:physiology*; D011235:Predatory Behavior; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000097:blood / Q000235:genetics; D013997:Time Factors
D004791:Enzyme Inhibitors; D005938:Glucocorticoids; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D001623:Betamethasone; D006854:Hydrocortisone; D008797:Metyrapone