Characterization of pectinesterase inhibitor in jelly fig (Ficus awkeotsang Makino) achenes.
Pectinesterase inhibitor (PEI) extract prepared from intact jelly fig (Ficus awkeotsang Makino) achenes was separated by membrane (MWCO 3 and 10 kDa) and fractionated by a Sepharose G-50 gel permeation chromatography. Results from Sepharose G-50 gel permeation chromatography and concanavalin A Sepharose chromatography revealed PEI as polypeptides with molecular weights ranging from 3.5 to 4.5 kDa. Incubation of a PE (1 unit/mL)-PEI (2 mg/mL) mixture for 1 min decreased the PE activity by approximately 50%. On the basis of the results of Lineweaver-Burk double-reciprocal plots, Michaelis constant (K(m)) and V(max) values for jelly fig achenes PE (pH 6.0, 30 degrees C) were 0.78 mM -OCH3 and 1.09 microequiv of -COOH/min, respectively. In addition, PEI competitively inhibited both citrus and jelly fig achenes PEs.
Jiang
Chii-Ming
CM
Department of Seafood Science, National Kaohsiung Institute of Marine Technology, Kaohsiung 811, Taiwan.
Li
Chia-Pei
CP
Chang
Jui-Chang
JC
Chang
Hung-Min
HM
D000596:Amino Acids / Q000032:analysis; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005591:Chemical Fractionation; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D003208:Concanavalin A; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D030681:Ficus / Q000737:chemistry*; D007700:Kinetics; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry; D037121:Plant Lectins
D000596:Amino Acids; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D037121:Plant Lectins; D003208:Concanavalin A; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase