Two ATP synthases can be linked through subunits i in the inner mitochondrial membrane of Saccharomyces cerevisiae.
Cross-linking experiments showed that the supernumerary subunit i is close to the interface between two ATP synthases. These data were used to demonstrate the presence of ATP synthase dimers in the inner mitochondrial membrane of Saccharomyces cerevisiae. A cysteine residue was introduced into the inter-membrane space located C-terminal part of subunit i. Cross-linking experiments revealed a dimerization of subunit i. This cross-linking occurred only with the dimeric form of the enzyme after incubating intact mitochondria with a bis-maleimide reagent, thus indicating an inter-ATP synthase cross-linking, whereas the monomeric form of the enzyme exhibited only an intra-ATP synthase cross-linking with subunit 6, another component of the membranous domain of the ATP synthase.
Paumard
Patrick
P
Institut de Biochimie et Génétique Cellulaires du CNRS, Université Victor Segalen, Bordeaux 2, 1 rue Camille Saint-Saëns 33077 Bordeaux Cedex, France.
Arselin
Geneviève
G
Vaillier
Jacques
J
Chaignepain
Stéphane
S
Bathany
Katell
K
Schmitter
Jean Marie
JM
Brèthes
Daniel
D
Velours
Jean
J
D000595:Amino Acid Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry*; D019281:Dimerization; D007425:Intracellular Membranes / Q000201:enzymology*; D008301:Maleimides / Q000737:chemistry; D008928:Mitochondria / Q000201:enzymology*; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry*; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
D003432:Cross-Linking Reagents; D008301:Maleimides; D029701:Saccharomyces cerevisiae Proteins; C011328:N,N'-4-phenylenedimaleimide; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; C023860:N,N'-2-phenylenedimaleimide