Role of ANC-1 in tethering nuclei to the actin cytoskeleton.
Mutations in anc-1 (nuclear anchorage defective) disrupt the positioning of nuclei and mitochondria in Caenorhabditis elegans. ANC-1 is shown to consist of mostly coiled regions with a nuclear envelope localization domain (called the KASH domain) and an actin-binding domain; this structure was conserved with the Drosophila protein Msp-300 and the mammalian Syne proteins. Antibodies against ANC-1 localized cytoplasmically and were enriched at the nuclear periphery in an UNC-84-dependent manner. Overexpression of the KASH domain or the actin-binding domain caused a dominant negative anchorage defect. Thus, ANC-1 may connect nuclei to the cytoskeleton by interacting with UNC-84 at the nuclear envelope and with actin in the cytoplasm.
Starr
Daniel A
DA
Howard Hughes Medical Institute and Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.
Han
Min
M
D000199:Actins / Q000378:metabolism*; D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029742:Caenorhabditis elegans Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000737:chemistry; D003599:Cytoskeleton / Q000378:metabolism*; D017238:Genes, Helminth; D008562:Membrane Glycoproteins / Q000378:metabolism; D008840:Microfilament Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000648:ultrastructure; D009154:Mutation; D009685:Nuclear Envelope / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010641:Phenotype; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism
C468285:ANC-1 protein, C elegans; D000199:Actins; D029742:Caenorhabditis elegans Proteins; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; C120560:Unc-84 protein, C elegans