title: Cold acclimation improves recovery of cryopreserved grass (Zoysia and Lolium sp.).
abstract: Cold acclimation of Lolium L. and Zoysia Willd. Grass cultivars significantly increased regrowth of cryopreserved meristems. One wk of cold acclimation improved recovery following cryopreservation but extended acclimation (4-8 wk) resulted in the best regrowth. Cold acclimation also significantly increased the dehydration tolerance of both Zoysia and Lolium meristems. Lolium apices cold acclimated for 4 wk produced 60-100% regrowth following cryopreservation by slow freezing or encapsulation-dehydration. Cold-acclimated Zoysia had greater than 60% regrowth following encapsulation-dehydration when beads were dehydrated to less than 22% water content. Non-acclimated meristems of both genera had little or no regrowth. Thawed meristems grew quickly without callus formation and the plantlets produced were transplanted to pots in the greenhouse after 4 to 6 wk. Samples of each cultivar were stored in liquid nitrogen as part of the U.S. National Plant Germplasm System.
authors.affiliation: Department of Horticulture, Oregon State University, Corvallis, OR 97331.
authors.forename: Y
authors.initials: Y
authors.lastname: Chang
authors.forename: R E
authors.initials: RE
authors.lastname: Barker
authors.forename: B M
authors.initials: BM
authors.lastname: Reed