title: Evaluation of methods for molecular typing and identification of members of the genus Brevibacterium and other related species.
abstract: The genus Brevibacterium includes pleomorphic Gram-positive bacteria with a high mol% G+C content. Species in the genus are difficult to identify by classical methods. The discriminatory power of DNA-based methods is assessed. Strains representing the four well established Brevibacterium species, and other related bacteria, were compared by amplified ribosomal DNA restriction analysis (ARDRA), repetitive-sequence-based PCR (rep-PCR) and ribotyping. Fingerprinting by rep-PCR and ribotyping provided complex genomic profiles with the highest discriminatory potential for molecular typing at the strain level, whereas ARDRA showed differentiation from the genus to the species levels. A high degree of heterogeneity within the genus Brevibacterium is apparent, thus indicating that the taxonomy of the genus should be further studied.
authors.affiliation: Centro de Biologia Celular, Campus Universitário de Santiago, Departamento de Biologia, Universidade de Aveiro, Portugal.
authors.forename: Artur
authors.initials: A
authors.lastname: Alves
authors.forename: Orlando
authors.initials: O
authors.lastname: Santos
authors.forename: Isabel
authors.initials: I
authors.lastname: Henriques
authors.forename: António
authors.initials: A
authors.lastname: Correia
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D001951:Brevibacterium / Q000145:classification* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D021521:Ribotyping
chemical_list: D004269:DNA, Bacterial