title: Synthesis of 99mTc-ciprofloxacin by different methods and its biodistribution.
abstract: We describe here the synthesis of 99mTc-ciprofloxacin by four different methods and its biodistribution. All of the methods gave high radiochemical yields of > or = 90% and high stability of > or = 90% at 6 h after preparation. However HPLC analysis, bacterial binding assay, and in vivo distribution for the four 99mTc-ciprofloxacins showed different results. Among these methods, the use of formamidine sulfinic acid with microwave heating (Method A) was fast and easy, and gave more desirable biological properties than the other methods.
authors.affiliation: Department of Nuclear Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Songpa-gu, Seoul, South Korea. sjoh@www.amc.seoul.kr
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Oh
authors.forename: Jin-Sook
authors.initials: JS
authors.lastname: Ryu
authors.forename: Joong Woo
authors.initials: JW
authors.lastname: Shin
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Yoon
authors.forename: Hyun-Joon
authors.initials: HJ
authors.lastname: Ha
authors.forename: Joon Hong
authors.initials: JH
authors.lastname: Cheon
authors.forename: Hee Kyung
authors.initials: HK
authors.lastname: Lee
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D008297:Male; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D011854:Radiochemistry; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D002939:Ciprofloxacin