Cloning and Expression of the pga Gene of Bacillus megaterium in E. coli.
The pga gene was amplified by PCR from Bacillus megaterium CA4098, cloned into pKK223-3 vector and expressed in E. coli HB101. The pga sequence was also determined. The deduced amino acids sequence was compared with those of PGAs from other bacteria and showed that they were similar, especially the active sites were strongly conserved.
Huang
Yan-Hong
YH
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
Zhang
Ying
Y
Chu
Ru-Ai
RA
Wu
Xiang-Fu
XF
Wang
Ying-Lai
YL
Yuan
Zhong-Yi
ZY