Influence of some formulation parameters on lysozyme adsorption and on its stability in solution.
According to our results concerning the behavior of lysozyme at interfaces, its secondary structure and its enzymatic activity, successful protein encapsulation would need to maintain a pH value far from the enzyme isoelectric point value during the formulation to reduce, in particular, the adsorption of lysozyme molecules at the created interfaces. Moreover, buffers or salt solution must be used in order to keep intact the native secondary conformation of lysozyme, and preserve its enzymatic activity.
Malzert
A
A
ERIT-M 0104 Ingénierie de la Vectorisation Particulaire, Immeuble IBT, 10, rue André Boquel, 49100, Angers, France.
Boury
F
F
Renard
D
D
Robert
P
P
Proust
J E
JE
Benoít
J P
JP
D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology; D004339:Drug Compounding; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008837:Micrococcus / Q000187:drug effects; D009113:Muramidase / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology; D011506:Proteins / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013500:Surface Tension
D000900:Anti-Bacterial Agents; D011506:Proteins; D009113:Muramidase