Crystallization and preliminary x-ray analysis of ovocleidin-17 a major protein of the gallus gallus eggshell calcified layer.
In this work, we report the crystallization of ovocleidin-17, the major protein of the avian eggshell calcified layer and the preliminary X-ray characterization of this soluble protein which is implied into the CaCO(3) formation of the eggshell in avians. Crystals belong to one of the trigonal space group P3 with cell dimensions a= b= 59.53 A and c = 83.33 A, and alpha=beta= 90 degrees and gamma=120 degrees. Crystals diffract up to 3.0 A.
Reyes-Grajeda
Juan Pablo
JP
Departamento de Bioquímica, Instituto de Química U.N.A.M. Circuito Exterior, C.U. México, D.F. 04510, México.
Jáuregui-Zúñiga
David
D
Rodríguez-Romero
Adela
A
Hernández-Santoyo
Alejandra
A
Bolanos-Garcia
Víctor M
VM
Moreno
Abel
A
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002645:Chickens; D018360:Crystallography, X-Ray; D004527:Egg Proteins / Q000737:chemistry*; D004528:Egg Shell / Q000737:chemistry*; D013696:Temperature; D014965:X-Rays
D004527:Egg Proteins; C095273:ovocleidin 17; D002118:Calcium