title: beta2-Integrin and lipid modifications indicate a non-antioxidant mechanism for the anti-atherogenic effect of dietary coenzyme Q10.
abstract: Dietary supplementation with coenzyme Q (CoQ) has been proposed to have anti-atherogenic effects by virtue of its antioxidant capacity. To investigate this question, the leukocyte status of 5 males and 5 females (52-68 years) was evaluated before and after supplementation with 200mg CoQ/day for 5 and 10 weeks. CoQ was selectively taken up by mononuclear cells and alpha-tocopherol increased in polynuclear and mononuclear cells. The expression of beta2-integrin CD11b and complement receptor CD35 on the plasma membrane of resting and stimulated monocytes was significantly decreased upon dietary CoQ. Fatty acid and aldehyde analysis revealed that there was a selective increase of arachidonic acid and plasmalogens in only mononuclear cells. These selective lipid changes are not consistent with a general improvement in antioxidant status and indicate that CoQ most likely inhibits a phospholipase A2. Thus, these results strongly suggest that the anti-atherogenic effects of CoQ be mediated by other mechanisms beside its antioxidant protection.
authors.affiliation: Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Turunen
authors.forename: Lena
authors.initials: L
authors.lastname: Wehlin
authors.forename: Mats
authors.initials: M
authors.lastname: Sjöberg
authors.forename: Joachim
authors.initials: J
authors.lastname: Lundahl
authors.forename: Gustav
authors.initials: G
authors.lastname: Dallner
authors.forename: Kerstin
authors.initials: K
authors.lastname: Brismar
authors.forename: Pavel J
authors.initials: PJ
authors.lastname: Sindelar
mesh_terms: D000368:Aged; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D018821:CD18 Antigens / Q000378:metabolism*; D003067:Coenzymes; D019610:Cytoprotection; D004036:Diet, Atherogenic; D019587:Dietary Supplements; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000378:metabolism*; D017463:Receptors, Complement 3b / Q000378:metabolism; D014451:Ubiquinone / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000378:metabolism; D024502:alpha-Tocopherol / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018821:CD18 Antigens; D003067:Coenzymes; D016177:Macrophage-1 Antigen; D010743:Phospholipids; D017463:Receptors, Complement 3b; D014451:Ubiquinone; C024989:coenzyme Q10; D024502:alpha-Tocopherol