Sesame oil attenuates multiple organ failure and increases survival rate during endotoxemia in rats.
OBJECTIVE
To investigate the effects and the possible mechanism of sesame oil on multiple organ failure induced by lipopolysaccharide in rats.


METHODS
Laboratory in vivo study of the effects of sesame oil on serum aspartate aminotransferase, gamma-glutamyltransferase, alkaline phosphatase, total bilirubin, blood urea nitrogen, creatinine, lipid peroxide, and nitric oxide concentrations. To assess the effect of sesame oil on xanthine oxidase, serum uric acid was measured. Furthermore, lipid peroxide concentrations in liver and kidney were determined.


METHODS
University laboratory.


METHODS
Male Wistar rats.


METHODS
Blood testing.


RESULTS
Serum aspartate aminotransferase, gamma-glutamyltransferase, alkaline phosphatase, total bilirubin, blood urea nitrogen, creatinine, and uric acid concentrations were determined. Lipid peroxide was analyzed by using a commercial kit. Nitric oxide production was estimated by Griess reaction. Sesame oil ameliorated hepatic and renal damage in a dose-dependent manner and increased animal survival in lipopolysaccharide-treated rats. Sesame oil decreased lipid peroxide concentration in serum but not in liver and kidney. Serum nitrite production was unaffected by sesame oil ingestion. Furthermore, the activity of xanthine oxidase was reduced by sesame oil in lipopolysaccharide-challenged rats.


CONCLUSIONS
Sesame oil ameliorated multiple organ failure and mortality via its inhibition of xanthine oxidase in lipopolysaccharide-dosed rats. Xanthine oxidase may play a critical role in sesame oil-associated organ protection during endotoxemia in rats.
Hsu
Dur-Zong
DZ
Graduate Institute of Environmental and Occupational Health, National Cheng Kung University Medical College, Tainan, Taiwan.
Liu
Ming-Yie
MY
D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D019446:Endotoxemia / Q000139:chemically induced / Q000188:drug therapy* / Q000401:mortality; D007668:Kidney / Q000098:blood supply / Q000378:metabolism; D008054:Lipid Peroxides / Q000097:blood; D008070:Lipopolysaccharides / Q000009:adverse effects; D008099:Liver / Q000098:blood supply / Q000378:metabolism; D008297:Male; D009102:Multiple Organ Failure / Q000139:chemically induced / Q000188:drug therapy* / Q000401:mortality; D009569:Nitric Oxide / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012715:Sesame Oil / Q000494:pharmacology*; D016019:Survival Analysis; D013997:Time Factors; D014527:Uric Acid / Q000097:blood
D008054:Lipid Peroxides; D008070:Lipopolysaccharides; D014527:Uric Acid; D009569:Nitric Oxide; D012715:Sesame Oil