title: Purification and characterization of a corrinoid compound from Chlorella tablets as an algal health food.
abstract: Vitamin B(12) content of an algal health food, Chlorella tablets (Chlorella sp.), was determined by both Lactobacillus leichmannii ATCC 7830 microbiological and intrinsic factor-chemiluminescence methods. The values of 200.9-211.6 microg/100 g dry weight determined by the chemiluminescence method were similar to the values (201.3-285.7 microg/100 g dry weight) determined by the microbiological method. A corrinoid compound was purified to homogeneity from the Chlorella tablets and characterized. The purified corrinoid compound was identified as vitamin B12, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV-Vis spectroscopy.
authors.affiliation: Department of Health Science, Hiroshima Prefectural Women's University, Hiroshima 734-8558, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kittaka-Katsura
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fujita
authors.forename: Fumio
authors.initials: F
authors.lastname: Watanabe
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Nakano
mesh_terms: D002708:Chlorella / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D045728:Corrinoids; D058870:Food, Organic / Q000032:analysis*; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D011166:Porphyrins / Q000737:chemistry* / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D014805:Vitamin B 12 / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D045728:Corrinoids; D011166:Porphyrins; D014805:Vitamin B 12