Cobalt ions influence proliferation and function of human osteoblast-like cells.
Cobalt is the major component in many orthopedic implants and the introduction of a second generation of metal on metal bearing prosthesis systems actualizes the toxicity and biocompatibility of this compound. We studied the effect of cobalt ions on primary cultures of human osteoblast-like cells. Cobalt ions dissolved in cell culture medium caused a dose-dependent decrease in proliferation of human osteoblasts measured as (3H)thymidine incorporation. We also found that cobalt ion-enriched medium increased the production of interleukin-6 from the osteoblast-like cells. Furthermore, incubation of osteoblasts with cobalt ion-enriched medium reduced collagen type I and osteocalcin production in a dose-dependent manner when 1,25 dihydroxyvitamin-D3 was added to the culture medium. Cobalt concentrations below 10 microg/mL or 0.17 mmollmL in the cell culture medium had no significant effect on human osteoblast proliferation and function.
Anissian
Lucas
L
Department of Orthopaedic Surgery, Karolinska Hospital and Karolinska Institutet, Stockholm, Sweden.
Stark
André
A
Dahlstrand
Henrik
H
Granberg
Barbro
B
Good
Victoria
V
Bucht
Elisabet
E
D002117:Calcitriol / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003035:Cobalt / Q000494:pharmacology*; D024042:Collagen Type I / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis
D024042:Collagen Type I; D015850:Interleukin-6; D015675:Osteocalcin; D003035:Cobalt; D002117:Calcitriol