Autoradiographically detected impairment of RNA synthesis pattern in 8-16 cell bovine embryos after irradiation.
Early preimplantation bovine embryos at 8- or 16-cell stage were analysed by [5-3H]uridine autoradiography for distribution of newly synthesized RNA after 60Co irradiation with a single dose of 1 Gy, 2 Gy or 4 Gy gamma rays, respectively. Embryos irradiated with a single dose of 1 Gy showed equally decreased synthesis of RNA in nucleoplasma as well as in nucleolus. In embryos irradiated with a single dose of 2 Gy or 4 Gy, RNA synthesis was decreased and localized mostly on the periphery of the nucleus; in both cases of irradiation, the nucleus center being without labelling. In most of embryos irradiated with a dose of 4 Gy, the nucleoli were not labelled, and an increasing occurrence appeared of various nucleus chromatin segregation forms, mainly as its marginalization.
Pivko
J
J
Research Institute of Animal Production, Institute of Genetics and Reproduction of Animals, Nitra, Slovakia. pivko@vuzv.sk
Kubovicová
E
E
Grafenau
P
P
Zibrín
M
M
Chlebovský
O
O
D000818:Animals; D001345:Autoradiography; D001755:Blastocyst / Q000473:pathology / Q000528:radiation effects*; D002417:Cattle; D002466:Cell Nucleolus / Q000473:pathology / Q000528:radiation effects; D002467:Cell Nucleus / Q000473:pathology / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D066298:In Vitro Techniques; D012313:RNA / Q000096:biosynthesis* / Q000528:radiation effects*; D011829:Radiation Dosage; D012680:Sensitivity and Specificity
D012313:RNA