Crystallization and preliminary x-ray studies of the Fab fragment from a humanized version of the mouse anti-human fas antibody HFE7a.
A humanized version of the apoptosis-inducing mouse anti-human Fas monoclonal antibody, HFE7A, is under further development for the treatment of autoimmune diseases such as rheumatoid arthritis. We have crystallized the antigen-binding fragment (Fab) of the humanized HFE7A. The crystals belong to the orthorhombic space group P2(1)2(1)2(1) with cell dimensions a = 54.4 A, b = 82.7 A, c = 104.9 A and contain one Fab molecule in the asymmetric unit. X-ray diffraction data were collected to 2.8 A resolution.
Ito
Shuichiro
S
Biomedical Research Laboratories, Sankyo, Co., Ltd., Shinagawa, Tokyo 140-8710, Japan.
Takayama
Tomoko
T
Hanzawa
Hiroyuki
H
Takahashi
Tohru
T
Miyadai
Kenji
K
Serizawa
Nobufusa
N
Haruyama
Hideyuki
H
Hata
Tadashi
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D058846:Antibodies, Monoclonal, Murine-Derived; D018360:Crystallography, X-Ray; D006801:Humans; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D051379:Mice; D019014:fas Receptor / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D058846:Antibodies, Monoclonal, Murine-Derived; D007128:Immunoglobulin Fragments; C411023:anti-Fas monoclonal antibody; D019014:fas Receptor