title: Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray.
abstract: Full-length cDNAs are essential for functional analysis of plant genes in the post-sequencing era of the Arabidopsis genome. Recently, cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles. We have prepared a full-length cDNA microarray containing approximately 7000 independent, full-length cDNA groups to analyse the expression profiles of genes under drought, cold (low temperature) and high-salinity stress conditions over time. The transcripts of 53, 277 and 194 genes increased after cold, drought and high-salinity treatments, respectively, more than fivefold compared with the control genes. We also identified many highly drought-, cold- or high-salinity- stress-inducible genes. However, we observed strong relationships in the expression of these stress-responsive genes based on Venn diagram analysis, and found 22 stress-inducible genes that responded to all three stresses. Several gene groups showing different expression profiles were identified by analysis of their expression patterns during stress-responsive gene induction. The cold-inducible genes were classified into at least two gene groups from their expression profiles. DREB1A was included in a group whose expression peaked at 2 h after cold treatment. Among the drought, cold or high-salinity stress-inducible genes identified, we found 40 transcription factor genes (corresponding to approximately 11% of all stress-inducible genes identified), suggesting that various transcriptional regulatory mechanisms function in the drought, cold or high-salinity stress signal transduction pathways.
authors.affiliation: Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan.
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
authors.forename: Mari
authors.initials: M
authors.lastname: Narusaka
authors.forename: Junko
authors.initials: J
authors.lastname: Ishida
authors.forename: Tokihiko
authors.initials: T
authors.lastname: Nanjo
authors.forename: Miki
authors.initials: M
authors.lastname: Fujita
authors.forename: Youko
authors.initials: Y
authors.lastname: Oono
authors.forename: Asako
authors.initials: A
authors.lastname: Kamiya
authors.forename: Maiko
authors.initials: M
authors.lastname: Nakajima
authors.forename: Akiko
authors.initials: A
authors.lastname: Enju
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Masakazu
authors.initials: M
authors.lastname: Satou
authors.forename: Kenji
authors.initials: K
authors.lastname: Akiyama
authors.forename: Teruaki
authors.initials: T
authors.lastname: Taji
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
authors.forename: Piero
authors.initials: P
authors.lastname: Carninci
authors.forename: Jun
authors.initials: J
authors.lastname: Kawai
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D003080:Cold Temperature; D003890:Desiccation; D004190:Disasters; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000139:chemically induced / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D018749:RNA, Plant / Q000032:analysis / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors; D015533:Transcriptional Activation
chemical_list: D018749:RNA, Plant; D012965:Sodium Chloride