title: [Analysis of human cytomegalovirus infection of an autopsy case of dermatomyositis with rapidly progressive interstitial pneumonia: the possible correlation between the viral genome and its related protein on the pathogenesis].
abstract: We describe a case of a 61-year-old woman with amyopathic dermatomyositis (ADM), who developed rapidly progressive interstitial pneumonia and died of respiratory failure. An autopsy revealed interstitial pneumonia with diffuse alveolar damage, associated with infiltration of T cells, mostly positive for CD 8. The alveolar lining epithelial cells manifested the remarkable expression of immediate early/early antigen of human cytomegalovirus (HCMV). Moreover, the extract of the lung was transmittable of HCMV infection to cultured human embryo-fibroblasts in vitro. On the other hand, in the semi-quantitative analysis of HCMV genome, using laser-assisted microdissection, followed by PCR method, the genomic DNA in the alveolar lining epithelial cells was little detected in this case, although it was remarkable in the case of immunodeficiency with cytomegalovirus pneumonia. This case may be important to know the role of the immune response of host to HCMV infection on the development of rapidly progressive interstitial pneumonia.
authors.affiliation: Second Department of Pathology of Ehime University School of Medicine, Shigenobu-cho, Onsen-gun, Ehime.
authors.forename: Norimasa
authors.initials: N
authors.lastname: Arita
authors.forename: Mitsuko R
authors.initials: MR
authors.lastname: Ito
authors.forename: Kimihiko
authors.initials: K
authors.lastname: Nakatani
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujii
authors.forename: Hisashi
authors.initials: H
authors.lastname: Ohishi
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Izumiyama
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Hirabayashi
authors.forename: Terunobu
authors.initials: T
authors.lastname: Saito
authors.forename: Masato
authors.initials: M
authors.lastname: Nose
mesh_terms: D003586:Cytomegalovirus Infections / Q000473:pathology*; D004279:DNA, Viral / Q000032:analysis; D003882:Dermatomyositis / Q000150:complications*; D005260:Female; D016679:Genome, Viral; D006801:Humans; D017563:Lung Diseases, Interstitial / Q000209:etiology* / Q000473:pathology / Q000821:virology*; D008875:Middle Aged; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral