title: Investigation of microsatellite instability in Turkish breast cancer patients.
abstract: Multiple somatic and inherited genetic changes that lead to loss of growth control may contribute to the development of breast cancer. Microsatellites are tandem repeats of simple sequences that occur abundantly and at random throughout most eucaryotic genomes. Microsatellite instability (MI), characterized by the presence of random contractions or expansions in the length of simple sequence repeats or microsatellites, is observed in a variety of tumors. The aim of this study was to compare tumor DNA fingerprints with constitutional DNA fingerprints to investigate changes specific to breast cancer and evaluate its correlation with clinical characteristics. Tumor and normal tissue samples of 38 patients with breast cancer were investigated by comparing PCR-amplified microsatellite sequences D2S443 and D21S1436. Microsatellite instability at D21S1436 and D2S443 was found in 5 (13%) and 7 (18%) patients, respectively. Two patients displayed instability at both marker loci. No association was found between MI and age, family history, lymph node involvement and other clinical parameters.
authors.affiliation: Department of Basic Oncology, Oncology Institute, Capa, Istanbul, 34390, Turkey. ndalay@yahoo.com
authors.forename: Semra
authors.initials: S
authors.lastname: Demokan
authors.forename: Mahmut
authors.initials: M
authors.lastname: Muslumanoglu
authors.forename: H
authors.initials: H
authors.lastname: Yazici
authors.forename: Abdullah
authors.initials: A
authors.lastname: Igci
authors.forename: Nejat
authors.initials: N
authors.lastname: Dalay
mesh_terms: D001943:Breast Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis*; D004276:DNA, Satellite / Q000235:genetics*; D005260:Female; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011379:Prognosis; D014421:Turkey / Q000453:epidemiology
chemical_list: D017931:DNA Primers; D004273:DNA, Neoplasm; D004276:DNA, Satellite