title: Simultaneous determination of binary mixtures of trimethoprim and sulfamethoxazole or sulphamethoxypyridazine by the bivariate calibration spectrophotometric method.
abstract: The bivariate calibration algorithm was applied to the spectrophotometric simultaneous determination of trimethoprim (TMP), sulfamethoxazole (SMX) or sulphamethoxypyridazine (SMP) binary mixtures in pharmaceutical and veterinary products. The results obtained were compared with those from derivative spectrophotometry. The statistical evaluation of the method bias showed that the proposed procedure is comparable with commonly used first-derivative spectrophotometry. However, the advantage of bivariate calibration is its simplicity, due to the minimal spectra manipulation when compared with derivative techniques.
authors.affiliation: Instituto de Investigaciones Científicas, Universidad De Guanajuato, GTO 36000, Guanajuato, Mexico.
authors.forename: Leticia
authors.initials: L
authors.lastname: López-Martínez
authors.forename: Pedro Luis
authors.initials: PL
authors.lastname: López-de-Alba
authors.forename: Luis Manuel
authors.initials: LM
authors.lastname: de-León-Rodríguez
authors.forename: Ma Lourdes
authors.initials: ML
authors.lastname: Yepez-Murrieta
mesh_terms: D000465:Algorithms; D002138:Calibration; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D016014:Linear Models; D013056:Spectrophotometry, Ultraviolet; D013421:Sulfamethoxypyridazine / Q000032:analysis*; D014295:Trimethoprim / Q000032:analysis*; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000032:analysis*
chemical_list: D004338:Drug Combinations; D007202:Indicators and Reagents; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D014295:Trimethoprim; D013421:Sulfamethoxypyridazine