title: Molecular breeding of transgenic rice plants expressing a bacterial chlorocatechol dioxygenase gene.
abstract: The cbnA gene encoding the chlorocatechol dioxygenase gene from Ralstonia eutropha NH9 was introduced into rice plants. The cbnA gene was expressed in transgenic rice plants under the control of a modified cauliflower mosaic virus 35S promoter. Western blot analysis using anti-CbnA protein indicated that the cbnA gene was expressed in leaf tissue, roots, culms, and seeds. Transgenic rice calluses expressing the cbnA gene converted 3-chlorocatechol to 2-chloromucote efficiently. Growth and morphology of the transgenic rice plants expressing the cbnA gene were not distinguished from those of control rice plants harboring only a Ti binary vector. It is thus possible to breed transgenic plants that degrade chloroaromatic compounds in soil and surface water.
authors.affiliation: Faculty of Bioresources, Mie University, Tsu 514-8507, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Shimizu
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kimura
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Koyama
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Suzuki
authors.forename: Naoto
authors.initials: N
authors.lastname: Ogawa
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Miyashita
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakka
authors.forename: Kunio
authors.initials: K
authors.lastname: Ohmiya
mesh_terms: D001673:Biodegradation, Environmental; D001947:Breeding / Q000379:methods*; D002396:Catechols / Q000378:metabolism; D020118:Cupriavidus necator / Q000201:enzymology / Q000235:genetics; D049308:Dioxygenases; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014170:Transformation, Genetic
chemical_list: D002396:Catechols; C031137:3-chlorocatechol; D010105:Oxygenases; D049308:Dioxygenases; C053101:catechol oxygenase II