Annexin-1 (lipocortin-1)-immunoreactivity in the folliculo-stellate cells of rat anterior pituitary: the effect of adrenalectomy and corticosterone treatment on its subcellular distribution.
In the pituitary gland, annexin-1 (lipocortin-1) located in folliculo-stellate (FS) cells has been advocated as one of the candidates for paracrine agents produced by FS cells that modulate the release of pituitary hormones. However, the expression and distribution pattern of annexin-1 in FS cells under different circulating corticosteroid conditions has not been examined. Thus, by means of pre-embedding immunoelectron microscopy, we investigated the expression of annexin-1 in FS cells under different corticosteroid conditions. Annexin-1-immunoreactivity was observed in the cytoplasm; especially intense immunoreactivity was detected in the follicle surface of FS cells under control conditions. After adrenalectomy, annexin-1-immunoreactivity almost disappeared, but the immunoreactivity recovered with corticosterone replacement. The expression of glucocorticoid receptor immunoreactivity in the nucleus of FS cells also showed a similar pattern to annexin-1 associated with the changes in the corticosteroid conditions. However, S-100 immunoreactivity, a marker for FS cells, was not changed whatever the corticosteroid conditions. These results confirm that glucocorticoids regulate the annexin-1 expression and demonstrate the translocation of annexin-1 from intracellular to pericellular sites in the FS cells of the rat anterior pituitary gland.
Ozawa
H
H
Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Kyoto, Japan. hozawa@basic.kpu-m.ac.jp
Miyachi
M
M
Ochiai
I
I
Tsuchiya
S
S
Morris
J F
JF
Kawata
M
M
D000315:Adrenalectomy; D000818:Animals; D017305:Annexin A1 / Q000032:analysis* / Q000187:drug effects; D003345:Corticosterone / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D010903:Pituitary Gland, Anterior / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis
D017305:Annexin A1; D011965:Receptors, Glucocorticoid; D009418:S100 Proteins; D003345:Corticosterone