Transfection with fluorinated lipoplexes based on fluorinated analogues of DOTMA, DMRIE and DPPES.
Fluorinated double-chain (poly)cationic lipids (one or both of these chains being ended by a highly fluorinated tail) which are close analogues of DOTMA, DMRIE or DPPES were designed as synthetic vectors for gene delivery. For N/P ratios (N=number of amine functions of the lipid; P=number of DNA phosphates) from 0.8 to 5, these fluorinated cationic lipids condensed DNA, with or without the use of DOPE, to form fluorinated lipoplexes. No specific cell toxicity was evidenced for these new fluorinated lipoplexes. The efficiency of some of the fluorinated lipoplexes to transfect lung epithelial A549 cells was comparable to that of the first generation of fluorinated lipoplexes made from fluorinated analogues of DOGS (Transfectam) [Bioconjug. Chem. 12 (2001) 114]. These results, combined with the higher in vivo transfection potential found for fluorinated lipoplexes than for conventional lipoplexes or PEI polyplexes [J. Gene Med. 3 (2001) 109], confirm that fluorinated lipoplexes are very promising gene transfer systems.
Gaucheron
Jérôme
J
Laboratoire de Chimie Bio-Organique, UMR 6001 CNRS, Université de Nice Sophia-Antipolis, Faculté des Sciences, B.P. 71, 06108 Nice Cédex 2, France.
Santaella
Catherine
C
Vierling
Pierre
P
D002460:Cell Line; D002470:Cell Survival; D004847:Epithelial Cells / Q000187:drug effects; D005461:Fluorine; D005822:Genetic Vectors; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008055:Lipids; D008081:Liposomes / Q000138:chemical synthesis; D015394:Molecular Structure; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D013096:Spermine / Q000031:analogs & derivatives* / Q000737:chemistry; D014162:Transfection / Q000379:methods*
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D008055:Lipids; D008081:Liposomes; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; C054005:N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium; C061134:dioctadecylamidoglycylspermine; C087276:(3-dimyristyloxypropyl)(dimethyl)(hydroxyethyl)ammonium; D005461:Fluorine; D013096:Spermine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; D005998:Glycine