[Method of orienting immobilized proteins on gold surface sensory modified with rhodanide].
The behavior of the biomolecules near the charged surface depending on the pH level of the solution was studied by the method of surface plasmon resonance. It was shown, that the preliminary modification of the surface by thiocyanat generating the effective negative charge at the surface allowed to immobilize protein molecule orientation depending on their charge state. Proteins keep their biological properties and form the oriented monolayer in the wide range of pH.
Boltovets
P N
PN
Institute of Microbiology and Virology, NAS of Ukraine, Kyiv. pboltovec@mail.ru
Diachenko
N S
NS
Snopok
B A
BA
Shirshov
Iu M
IuM
D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013861:Thiocyanates / Q000737:chemistry*
D011506:Proteins; D013861:Thiocyanates; D006046:Gold; C009941:potassium thiocyanate