The mechanism of type IA topoisomerases.
The topology of cellular DNA is carefully controlled by enzymes called topoisomerases. By using single-molecule techniques, we monitored the activity of two type IA topoisomerases in real time under conditions in which single relaxation events were detected. The strict one-at-a-time removal of supercoils we observed establishes that these enzymes use an enzyme-bridged strand-passage mechanism that is well suited to their physiological roles and demonstrates a mechanistic unity with type II topoisomerases.
Dekker
N H
NH
Laboratoire de Physique Statistique et Département de Biologie, Ecole Normale Supérieure, Unité Mixte de Recherche 8550 Centre National de la Recherche Scientifique, 24 Rue Lhomond, 75231 Paris, France. Nynke.Dekker@lps.ens.fr
Rybenkov
V V
VV
Duguet
M
M
Crisona
N J
NJ
Cozzarelli
N R
NR
Bensimon
D
D
Croquette
V
V
D055592:Biophysical Phenomena; D001703:Biophysics; D004264:DNA Topoisomerases, Type I / Q000737:chemistry* / Q000378:metabolism*; D004278:DNA, Superhelical / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020124:Thermotoga maritima / Q000201:enzymology / Q000235:genetics
D004278:DNA, Superhelical; D011994:Recombinant Proteins; D004264:DNA Topoisomerases, Type I