Differential expression of Prx I and II in mouse testis and their up-regulation by radiation.
Testis is one of the most sensitive organs to ionizing radiation. The present study was designed to unravel the possible role of antioxidant proteins, peroxiredoxin I and II (Prx I and II) in the testis. Our results show that Prx I and II are constitutively expressed in the testis and their expression levels are decreased to some extent as the testis develops. Interestingly, immunohistochemical analysis revealed a preferential expression of Prx I and II in Leydig and Sertoli cells, respectively. Neither Prx I nor Prx II expression was obvious in the testicular germ cells including spermatogonia and spermatocytes. Ionizing radiation exerted oxidative stress on the testis and induced apoptosis primarily in the germ cells. When the irradiated testis was examined, the Prx system was found to be transiently up-regulated. Taken together, we suggest that the relative radiation-resistance of Leydig and Sertoli cells could be attributed in part to the antioxidant function of the Prx system in these cells.
Lee
Keesook
K
Hormone Research Center, Chonnam National University, Gwangju, Republic of Korea.
Park
Ji-Sun
JS
Kim
Yun-Jeong
YJ
Soo Lee
Yong Soo
YS
Sook Hwang
Tae Sook
TS
Kim
Dae-Joong
DJ
Park
Eun-Mi
EM
Park
Young-Mee
YM
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D005978:Glutathione / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D054464:Peroxiredoxins; D013737:Testis / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D015854:Up-Regulation / Q000528:radiation effects*; D014965:X-Rays
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D010544:Peroxidases; D054464:Peroxiredoxins; D005978:Glutathione