Feline chlamydiosis in Italy: PCR amplification and analysis of the ompA and groEL-homolog genes.
The ompA and groEL-homolog genes of Chlamydia psittaci were amplified from feline conjunctival swabs. The PCR products from positive samples for ompA and groEL genes were digested with the restriction enzyme Alu I and Hind III, respectively. In addition, the PCR products from both genes were sequenced. The results confirm the high homogeneity of the ompA gene of feline C. psittaci. The RFLP analysis of groEL-homolog gene could be useful to typing the strains of feline C. psittaci.
Di Francesco
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A
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale, Università di Bologna, Ozzano Emilia, BO, Italy.
Baldelli
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R
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D018834:Chaperonin 60 / Q000737:chemistry / Q000235:genetics*; D002690:Chlamydia Infections / Q000382:microbiology / Q000662:veterinary*; D002691:Chlamydophila psittaci / Q000145:classification / Q000235:genetics*; D003234:Conjunctivitis, Bacterial / Q000382:microbiology / Q000662:veterinary*; D007558:Italy; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
D001425:Bacterial Outer Membrane Proteins; D018834:Chaperonin 60; C078167:OMPA outer membrane proteins