A novel pyrrolidinyl PNA showing high sequence specificity and preferential binding to DNA over RNA.
A novel conformationally constrained pyrrolidinyl peptide nucleic acid (PNA) carrying an D-aminopyrrolidine carboxylic acid (D-Apc) spacer was synthesized, and its interactions with complementary oligo- and polynucleotides were studied by UV and CD spectroscopy. The decathymine PNA formed very stable PNA-DNA complexes with poly(dA) and (dA)(10) by a sequence-specific A-T pairing. The interaction with poly(rA) gave the corresponding PNA-RNA complex with much lower stability.
Vilaivan
Tirayut
T
Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Patumwan, Bangkok 10330, Thailand.
Lowe
Gordon
G
D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D020135:Peptide Nucleic Acids / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet
D020135:Peptide Nucleic Acids; D012313:RNA; D004247:DNA