Glucocorticoid-attenuated response genes induced in the lung during endotoxemia.
Cytokines and other mediators whose induction in inflammatory lung disease is attenuated by glucocorticoids are potential targets for development of selective anti-inflammatory treatments. We refer to genes with these regulatory characteristics as glucocorticoid-attenuated response genes, or GARGs. Systematic identification of GARGs has not been attempted previously in vivo. Using an endotoxemia model in adrenalectomized mice, we constructed a subtracted lung library enriched in endotoxemia-induced genes and identified candidate GARGs by differential hybridization screening. Northern analysis confirmed induction in the lung during endotoxemia and attenuation by glucocorticoids of 36 genes of diverse types. The majority were genes of unknown function not previously implicated in the pulmonary response to inflammation, including a new member of a 2'-5'-oligoadenylate synthetase-like family and a novel lung inducible Neuralized-related C3HC4 RING protein. Our results suggest that a full understanding of glucocorticoid effects on lung inflammation will require elucidation of the roles of an extensive network of glucocorticoid-modulated genes.
Smith
Jeffrey B
JB
Department of Pediatrics, UCLA School of Medicine and Mattel Children's Hospital at UCLA, University of California, 90095, USA. JBSmith@ucla.edu
Nguyen
Tam T
TT
Hughes
Heather J
HJ
Herschman
Harvey R
HR
Widney
Daniel P
DP
Bui
Kim C
KC
Rovai
Leonor E
LE
D015088:2',5'-Oligoadenylate Synthetase; D000315:Adrenalectomy; D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D018076:DNA, Complementary / Q000187:drug effects / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D019446:Endotoxemia / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D005796:Genes / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D008168:Lung / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D044767:Ubiquitin-Protein Ligases
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D005938:Glucocorticoids; D011506:Proteins; C465118:Neurl3 protein, mouse; D044767:Ubiquitin-Protein Ligases; C115020:OASL protein, human; D015088:2',5'-Oligoadenylate Synthetase