Evaluation of Fumonisin B(1) and its metabolites absorption and toxicity on intestinal cells line Caco-2.
The aim of the present paper is to investigate intestinal absorption and toxicity of Fumonisin B(1) (FB(1)) and its partially (PHFB(1) and PHFB(2)) and totally hydrolyzed (HFB(1)) metabolites, using the human intestinal cell line Caco-2, a very well known in vitro model of intestinal epithelium for absorption and metabolism studies. Caco-2 cells were treated for 48 h with several toxin concentrations (in the range of 1-138 microM). At the end of exposure period, no significant variation on cell viability has been observed with all chemicals tested, either in undifferentiated cells or in differentiated ones, suggesting a poor toxicity of these mycotoxins for intestinal cells. In any case, FB(1) appears the most active in this respect. For which concerns the cellular absorption, FB(1), PHFB(1) and PHFB(2) are never detected into Caco-2 cells. On the contrary, a dose-dependent absorption of HFB(1) has been observed in differentiated cells, which express enzymatic and metabolic characteristics of mature enterocytes. Thus HFB(1), losing the tricarballylic acid chain, is more bioavailable than FB(1) on intestinal cell, supporting the hypothesis that in risk evaluation of fumonisins exposure its metabolites are also relevant.
Caloni
F
F
Department of Veterinary Sciences and Technologies for Food Safety, University of Milan, Via Celoria 10, 20133, Milan, Italy. francesca.caloni@unimi.it
Spotti
M
M
Pompa
G
G
Zucco
F
F
Stammati
A
A
De Angelis
I
I
D001711:Biotransformation; D018938:Caco-2 Cells; D002274:Carcinogens, Environmental / Q000493:pharmacokinetics / Q000633:toxicity*; D002454:Cell Differentiation / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003114:Colony-Forming Units Assay; D037341:Fumonisins / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*; D006801:Humans; D006868:Hydrolysis; D007408:Intestinal Absorption / Q000187:drug effects*
D002274:Carcinogens, Environmental; D037341:Fumonisins; C056933:fumonisin B1