title: A direct sandwich ELISA to detect antibodies against the C-terminal region of merozoite surface protein 1 could be a useful diagnostic method to identify Plasmodium vivax exposed persons.
abstract: We expressed a C-terminal 108-aa region of the Plasmodium vivax merozoite surface protein 1 (PvMSP1c) excluding the C-end transmembrane region in Escherichia coli in order to evaluate the antibody level to MSP in Korean malaria patients. We optimized a direct sandwich enzyme-linked immunosorbent assay (ELISA) method to simultaneously determine the total antibody levels, including IgG and IgM, to PvMSP1c. If the cut-off for seropositivity was determined as the mean+3SD of the antibody levels of the negative control group, the antibody levels were positive in 99.5% of the patient group (sensitivity 199/200). The antibody levels were negative in 99.4% of the negative control group (specificity 504/507). The positive reactions in the negative control group came from non-specific reactions, as confirmed by a competition assay. This direct sandwich ELISA for PvMSP1c antibody could prove to be a useful tool for the diagnosis of malaria patients and for blood screening in blood banks.
authors.affiliation: Department of Biochemistry, College of Science, Protein Network Research Center, Yonsei University, Seoul, 120-749, Republic of Korea.
authors.forename: Kook Jin
authors.initials: KJ
authors.lastname: Lim
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Park
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Sohn
authors.forename: Seungwon
authors.initials: S
authors.lastname: Lee
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Oh
authors.forename: Hyung Cheol
authors.initials: HC
authors.lastname: Kim
authors.forename: Young Yil
authors.initials: YY
authors.lastname: Bahk
authors.forename: Yu Sam
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000097:blood; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D016780:Malaria, Vivax / Q000175:diagnosis*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000276:immunology* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000276:immunology; D015203:Reproducibility of Results; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D000913:Antibodies, Protozoan; D020066:Merozoite Surface Protein 1; D011993:Recombinant Fusion Proteins