Involvement of Oct-1 in transcriptional regulation of beta-casein gene expression in mouse mammary gland.
Mouse beta-casein gene promoter contains a region termed block C which is crucial for its gene transcription induced by lactogenic hormones. Nuclear extracts from mouse mammary glands contain at least two binding complexes (DS1 and DS2) which specifically bind to double-stranded block C region DNA. The binding sequence of these complexes was identified to be 5'-AAATTAGCATGT-3' which contains a sequence element related to the consensus octamer motif's complement ATTTGCAT. In the present study, we demonstrate that this sequence element indeed is the binding site for octamer-binding transcription factors (Octs) and Octs represent the double-stranded DNA binding proteins specifically binding to the block C region. Formation of the specific double-stranded binding complexes can be completely blocked by Oct binding motif oligonucleotides and anti-rOct-1 antiserum. We also show that Oct-1B represents at least partial, if not all, double-stranded binding protein, DS1, in mammary nuclear extract. Oct-1B may function as a transcriptional activator on casein gene promoter. The Oct binding activity to beta-casein gene promoter in the mammary gland is affected under influence of hormones both in vitro and in vivo. The DS1 binding activity can be induced by the combination of lactogenic hormones insulin, hydrocortisone and prolactin in organ culture of virgin mouse mammary gland. The binding activity in vivo can be induced by injection of progesterone or its combination with estradiol in virgin mice.
Zhao
Feng-Qi
FQ
Laboratory of Genetics and Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Adachi
Kazushige
K
Oka
Takami
T
D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002364:Caseins / Q000096:biosynthesis / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006728:Hormones / Q000494:pharmacology; D051863:Host Cell Factor C1; D007106:Immune Sera / Q000494:pharmacology; D046911:Macromolecular Substances; D008321:Mammary Glands, Animal / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008894:Milk Proteins; D050811:Octamer Transcription Factor-1; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000235:genetics; D020218:Response Elements; D050799:STAT5 Transcription Factor; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D015533:Transcriptional Activation
D002364:Caseins; D004268:DNA-Binding Proteins; C497693:Hcfc1 protein, mouse; D006728:Hormones; D051863:Host Cell Factor C1; D007106:Immune Sera; D046911:Macromolecular Substances; D008894:Milk Proteins; D050811:Octamer Transcription Factor-1; C494166:Pou2f1 protein, mouse; D020033:Protein Isoforms; D050799:STAT5 Transcription Factor; D015534:Trans-Activators; D014157:Transcription Factors