Boric acid susceptibility testing of non-C. albicans Candida and Saccharomyces cerevisiae: comparison of three methods.
To establish the best method for boric acid susceptibility testing, we compared two agar dilution methods (high and low inoculum) and a standard broth microdilution method (from the National Commitee for Clinical Laboratory Standards document NCCLS M-27A). Saccharomyces cerevisiae (37) and non-C. albicans Candida (39) isolates, as well as one isolate of Trichosporon sp., were included. All were isolated from female workers with vulvovaginitis. Good agreement within a fourfold dilution range was found between the three methods, and only the broth microdilution method versus the agar dilution method with high inoculum showed significant discrepancies. Reading results was easier with the broth microdilution method than with the agar dilution methods because of partial growth inhibition in the latter. In conclusion, broth microdilution is a suitable method for testing yeast susceptibility to boric acid.
Otero
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L
Servicio de Microbiología, Hospital de Cabueñes, Gijón, Spain.
Palacio
V
V
Mendez
F J
FJ
Vazquez
F
F
D000362:Agar; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001888:Boric Acids / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology; D003470:Culture Media; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009181:Mycoses / Q000382:microbiology; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000302:isolation & purification; D014250:Trichosporon / Q000187:drug effects / Q000302:isolation & purification; D014848:Vulvovaginitis / Q000382:microbiology
D000891:Anti-Infective Agents, Local; D000935:Antifungal Agents; D001888:Boric Acids; D003470:Culture Media; D000362:Agar; C032688:boric acid