Staurosporine enhances the expression of tissue inhibitor of metalloproteinase-1 in human prostate cancer cells.
We reported previously that human prostate cancer cell line TSU-Pr1 can differentiate into neuronal cells by staurosporine treatment. In this process, reduction of invasive abilities was observed in staurosporine treated TSU-Pr1 cells. In the present study, we investigated the effect of staurosporine on tissue inhibitor of metalloproteinases (TIMPs) in prostate cancer cells. We show that treatment of TSU-Pr1 cells with staurosporine results in induction of TIMP-1 mRNA and protein secretion. The induction of TIMP-1 mRNA expression by staurosporine is likely to be caused by increased transcriptional activity and this mechanism is indirect. Furthermore, recombinant human TIMP-1 reduces the invasive activity of TSU-Pr1 cells. We are the first to report that mRNA expression and protein secretion of TIMP-1 are enhanced by staurosporine treatment in prostate cancer cells. These findings suggest that enhancement of TIMP-1 is associated with suppression of invasive activity caused by staurosporine treatment.
Tachibana
Ken
K
Department of Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 12 Ichigaya, Funagawara-machi, Shinjuku-ku, Tokyo 162-0826, Japan.
Shimizu
Takahisa
T
Tonami
Kazuo
K
Takeda
Ken
K
D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D019311:Staurosporine / Q000494:pharmacology*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019715:Tissue Inhibitor of Metalloproteinase-1; D019311:Staurosporine