title: Specific fluorescent tracers. Imaging and applications for photodynamic therapy.
abstract: Our main objective is to enlarge the fluorescence use in biosciences, with especially the photodynamic therapy (PDT) used for cancer treatment as one of the target applications. Meta-tetra(hydroxyphenyl)chlorin (m-THPC) is a second-generation photosensitiser, applied in photodynamic therapy. The localisation of this sensitiser as well as its induced cell death mechanisms in human breast cancer cells (MCF-7 and its resistant subline MCF-7DXR, DXR: doxorubicin) were evaluated using fluorescence microscopy. In addition, we will present two additional routes, whose aims are to create new features to respond to the PDT questioning: firstly, the synthesis of fluorescent tracers, with a particular attention to the presence of hydrophilic groups (glucosamine ring) on the basic fluorophore structure to orientate the localisation of the probe and, secondly, the use of scanning near-field optical microscopy to reach a better resolution for the fluorescence microscopy analysis.
authors.affiliation: CRAN-IMAC, UMR 7039 CNRS-UHP-INPL, centre Alexis-Vautrin, 54500 Vandoeuvre-les-Nancy, France.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Teiten
authors.forename: Pascale
authors.initials: P
authors.lastname: Even
authors.forename: Pierre
authors.initials: P
authors.lastname: Burgos
authors.forename: Céline
authors.initials: C
authors.lastname: Frochot
authors.forename: Sébastien
authors.initials: S
authors.lastname: Aubert
authors.forename: Marie-Christiane
authors.initials: MC
authors.lastname: Carré
authors.forename: Lina
authors.initials: L
authors.lastname: Bolotine
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Merlin
authors.forename: François
authors.initials: F
authors.lastname: Guillemin
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Viriot
mesh_terms: D001692:Biological Transport; D001943:Breast Neoplasms / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D004273:DNA, Neoplasm / Q000378:metabolism; D019008:Drug Resistance, Neoplasm; D005260:Female; D005456:Fluorescent Dyes; D006031:Glycosylation; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D008652:Mesoporphyrins / Q000493:pharmacokinetics / Q000633:toxicity; D008856:Microscopy, Fluorescence / Q000379:methods*; D009369:Neoplasms / Q000188:drug therapy; D010778:Photochemotherapy / Q000379:methods*; D011166:Porphyrins / Q000737:chemistry; D011838:Radiation-Sensitizing Agents / Q000493:pharmacokinetics / Q000633:toxicity; D013347:Subcellular Fractions / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; D008652:Mesoporphyrins; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C072269:temoporfin