title: Continuous nasal administration of antigen is critical to maintain tolerance in adoptively transferred autoimmune arthritis in SCID mice.
abstract: Mucosal tolerance is a natural mechanism that prevents immunological reactions to antigens by altering the activity of immune cells of pathogenic clones without modulating the entire immune system. This 'natural immune suppression' can be exploited when antigen(s) of the target organ in an autoimmune disease is used for mucosal treatment. Being inspired by the experimental results in animal models, clinical trials using type II collagen for mucosal treatment have been conducted in rheumatoid arthritis. High-density proteoglycan (aggrecan) is another major macromolecular component in articular cartilage, and may be a candidate autoantigen for provoking immune reactions in patients with rheumatoid arthritis. Indeed, like type II collagen, systemic immunization of genetically susceptible mice with proteoglycan (PG) aggrecan induces progressive autoimmune polyarthritis. Here, we investigated whether intranasally applied PG can be effective in suppressing PG-induced arthritis (PGIA) in BALB/c mice. We found that nasal administration of 100 microg PG exerted a strong suppressive effect on both the incidence and severity of the disease, most probably by reducing responsiveness towards the immunizing PG antigen. When we transferred PGIA into genetically matched but immunodeficient SCID mice, we were able to establish a tolerized state, but only if the recipient SCID mice received lymphocytes from tolerized animals and intranasal treatment with PG was continued. Without nasally administered antigen, the transferred anergic cells recovered and arthritis rapidly developed in a severe form. Intranasal PG treatment of recipient SCID mice was ineffective when cells from non-tolerized arthritic donors were transferred, in which case the regular weekly 'tolerizing' dose of PG made the disease worse. Our results suggest that mucosal treatment in an already existing disease may result in paradoxical outcomes.
authors.affiliation: Department of Orthopaedic Surgery and Biochemistry, Rush University at Rush-Presbyterian-St Luke's Medical Center, Chicago, Illinois 60612, USA.
authors.forename: T
authors.initials: T
authors.lastname: Bárdos
authors.forename: M
authors.initials: M
authors.lastname: Czipri
authors.forename: C
authors.initials: C
authors.lastname: Vermes
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: K
authors.initials: K
authors.lastname: Mikecz
authors.forename: T T
authors.initials: TT
authors.lastname: Glant
mesh_terms: D000281:Administration, Intranasal; D019264:Adoptive Transfer; D053674:Aggrecans; D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D001169:Arthritis, Experimental / Q000276:immunology* / Q000628:therapy*; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000628:therapy; D015551:Autoimmunity; D024043:Collagen Type II / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D016326:Extracellular Matrix Proteins; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D018928:Immunity, Mucosal; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000096:biosynthesis; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D011509:Proteoglycans / Q000008:administration & dosage / Q000276:immunology; D017634:Self Tolerance; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: C508320:Acan protein, mouse; D053674:Aggrecans; D000941:Antigens; D024043:Collagen Type II; D016207:Cytokines; D016326:Extracellular Matrix Proteins; D007376:Interleukin-2; D037181:Lectins, C-Type; D011509:Proteoglycans