[A new oxidase method for analyzing L-lactate].
A new oxidase-coupled colorimetric method for analysis of L-lactate in biological fluids has been developed without use of peroxidase. The method is based on lactate oxidase-catalysed transformation of lactate to pyruvate which is determined photometrically in the next dye-producing reaction of 3-methyl-2-benzothiazolinone hydrazone (MBTH) in the presence of ferric ions. Sensitivity of the method is estimated as 0.1 micromole of analyte in 4-ml of reaction mixture. Linearity is observed in the range 0.1-1.0 micromole of L-lactate in sample (r = 0.99943; p < 0.0001). The developed method has been adapted for assay of L-lactic acid in kefirs and yogurts.
Pavlishko
H M
HM
Institute of Cell Biology, NAS of Ukraine, Lviv.
Maĭdan
M M
MM
Sybirnyĭ
A A
AA
Honchar
M V
MV
D002384:Catalysis; D003124:Colorimetry; D019344:Lactic Acid / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D012680:Sensitivity and Specificity
D019344:Lactic Acid; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase