Preparative continuous annular chromatography (P-CAC) enables the large-scale fractionation of fructans.
Fructans (fructo-oligosaccharides and inulin) are of increasing physiological and nutritional interest due to their health-promoting effects. Fructans originally extracted from chicory roots were separated by continuous annular and fixed-bed conventional gel chromatography. Both columns were packed with Toyopearl HW 40 (S) and eluted with deionized water. A multicomponent fractionation was established to obtain single oligosaccharides in a low molecular weight range up to a chain length of five and fractions containing an overall size distribution in the high molecular weight range up to a chain length of 90 monosaccharide units. The productivity and resolution of the continuous annular size exclusion chromatograph (40 cm bed height) were investigated and compared with those of the fixed-bed counterpart (2 x 100 cm bed height). The eluting fractions were analyzed by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The productivity of the annular system was found to be 25-fold higher than the conventional system. Thus, annular chromatography exemplified for the fractionation of fructans is a powerful method for the large-scale and continuous fractionation of oligomeric and polymeric carbohydrates.
Finke
Berndt
B
Numico Research Germany, Bahnstrasse 14-30, D-61381 Friedrichsdorf, Germany. berndt.finke@onlinehome.de
Stahl
Bernd
B
Pritschet
Markus
M
Facius
Dirk
D
Wolfgang
Jürgen
J
Boehm
Guenther
G
D005591:Chemical Fractionation; D018651:Cichorium intybus / Q000737:chemistry; D002845:Chromatography / Q000379:methods*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005630:Fructans / Q000302:isolation & purification*; D008970:Molecular Weight; D018517:Plant Roots / Q000737:chemistry
D005630:Fructans