Antioxidative activity of spin labeled derivatives of podophyllic acid hydrazide.
OBJECTIVE
To study the relationship between structure and antioxidation activity of spin labeled derivatives of podophyllic acid hydrazide (GP) in tissues and red blood cells (RBC) from rats.


METHODS
The homogenate of liver, heart, and kidneys of rats was used to measure malondialdehyde (MDA) spontaneous generated and induced by hydroxyl free radical generation system (Fe2+-ascorbic acid, FRGS) or doxorubicin (DOX) by TBA colorimetric method. H2O2-caused hemolysis was determined spectrophotometrically. Superoxide anion from zymosan-stimulated neutrophils of rats was evaluated by NBT-reduction assay.


RESULTS
GP1 and GP1OH obviously inhibited MDA formation either spontaneously or induced by FRGS and DOX and antagonized hemolysis induced by H2O2, but GP and GP1H showed less potent activity. GP1 also inhibited the formation of superoxide anion from activated neutrophils of rats.


CONCLUSIONS
Introduction of nitroxyl radical moieties into GP generated potent derivatives with antioxidative activity. The essential antioxidation active groups of spin labeled derivative of GP are NO or NOH group in nitroxyl radical moieties.
Li
Wen-Guang
WG
Department of Pharmacology, Department of Physiology, Lanzhou Medical College, Lanzhou 730000, China. wyj@lz.gs.cninfo.net
Zhang
Xiao-Yu
XY
Wu
Yong-Jie
YJ
Tian
Xuan
X
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D011033:Podophyllin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011034:Podophyllotoxin / Q000031:analogs & derivatives; D051381:Rats; D017208:Rats, Wistar; D013113:Spin Labels; D019097:Spin Trapping
D000975:Antioxidants; D013113:Spin Labels; D008315:Malondialdehyde; C003170:mitopodozide; D011033:Podophyllin; D011034:Podophyllotoxin