IL-1beta gene polymorphisms influence hepatitis B vaccination.
Considerable variability exists in the vaccine response to hepatitis B with 5-10% of healthy young adults demonstrating no or inadequate responses following a standard vaccination schedule. As the interleukin-1beta (IL-1beta) cytokine has been shown to be important in the development of immune responses, we determined whether vaccine efficacy is influenced by genetic polymorphisms associated with IL-1beta expression. Ninety-two healthy individuals who were negative for antibodies to hepatitis B antigen (anti-HBs) were vaccinated against hepatitis B according to a standardized schedule. At selected times, antibody titers and lymphoproliferative capacity to hepatitis B surface antigen (HBsAg) were determined. DNA genotyping for IL-1beta polymorphisms using a polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) technique demonstrated that both the anti-HBs titer and the T-cell lymphoproliferative response to HBsAg are significantly increased in individuals possessing the IL-1beta (+3953) minor allelic variant.
Yucesoy
Berran
B
National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26508, USA.
Sleijffers
Annemarie
A
Kashon
Michael
M
Garssen
Johan
J
de Gruijl
Frank R
FR
Boland
Greet J
GJ
van Hattum
Jan
J
Simeonova
Petia P
PP
Luster
Michael I
MI
van Loveren
Henk
H
D000328:Adult; D000483:Alleles; D000917:Antibody Formation / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D006510:Hepatitis B Antibodies / Q000096:biosynthesis; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007375:Interleukin-1 / Q000235:genetics* / Q000502:physiology; D008213:Lymphocyte Activation / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D014614:Vaccines, Synthetic / Q000276:immunology
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D007375:Interleukin-1; D014614:Vaccines, Synthetic