The effect of netilmicin and vancomycin on lipid peroxidation processes in cerebrospinal fluid in children with hydrocephalus.
In biological systems, it is difficult to determine free radicals because of their reactivity and their very short time of existence. On the basis of markers, which come into being as a result of radical processes, one might believe that there exist reactive oxygen species. One of the determinants of free radical activity of oxygen is the presence of malondialdehyde (MDA), a final product of lipid peroxidation. This study aimed at finding the answer to the question whether the concentration of netylmicin and vancomycin influences the amount of substances reacting with thiobarbituric acid (TBA) in cerebrospinal fluid (CSF) in children with hydrocephalus. Applying the TBA test for examinations with antibiotics added both in vivo and in vitro, we could demonstrate that increased concentration of the examined antibiotics in cerebrospinal fluid reduces the amount of MDA. The results obtained demonstrate that products of lipid peroxidation are present in the CSF samples analyzed. In this study, we found that the concentration of vancomycin and netilmicin influenced the lipid peroxidation process in cerebrospinal fluid in children with hydrocephalus, thus confirming anti-inflammatory properties of the antibiotics applied.
Radwańska-Wala
Bozena
B
Department of Pharmaceutical Chemistry, Medical University of Silesia, Sosnowiec, Poland.
Buszman
Ewa
E
Bafeltowska
Jolanta
J
Wrześniok
Dorota
D
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000134:cerebrospinal fluid*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D006849:Hydrocephalus / Q000134:cerebrospinal fluid*; D007276:Injections, Intraventricular; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000032:analysis; D009428:Netilmicin / Q000008:administration & dosage / Q000134:cerebrospinal fluid*; D013858:Thiobarbiturates / Q000032:analysis; D014640:Vancomycin / Q000008:administration & dosage / Q000134:cerebrospinal fluid*
D000900:Anti-Bacterial Agents; D013858:Thiobarbiturates; D009428:Netilmicin; D008315:Malondialdehyde; D014640:Vancomycin; C029684:thiobarbituric acid