Human neural stem cells: electrophysiological properties of voltage-gated ion channels.
We have characterized the profile of membrane currents in an immortalized human neural stem cell line, HB1.F3 cells, using whole-cell patch clamp technique. Human neural stem cell line generated from primary cell cultures of embryonic human telencephalon using a replication-incompetent retroviral vector containing v-myc expresses nestin, a cell type-specific marker for neural stem cells. The human neural stem cells expressed both outward and inward K(+) currents with no evidence for Na(+) currents. The density of the outward, delayed rectifying type K(+) current was 1.8 +/- 0.015 nA/pF, and that of the inwardly rectifying K(+) current was 0.37 +/- 0.012 nA/pF (at 30 mM of [K(+)](o)). In order to induce neuronal differentiation of the neural stem cells, a full-length coding region of NeuroD, a neurogenic transcription factor, was transfected into HB1.F3 cells. Introduction of NeuroD induced expression of Na(+) currents with the current density of 0.042 +/- 0.011 nA/pF. The presence of two types of K(+) currents and expression of Na(+) currents induced by NeuroD appear to reflect the characteristic physiological features of human neural stem cells.
Cho
T
T
Brain Disease Research Center, Ajou University, Suwon 442-749, Korea.
Bae
J H
JH
Choi
H B
HB
Kim
S S
SS
McLarnon
J G
JG
Suh-Kim
H
H
Kim
S U
SU
Min
C K
CK
D051792:Basic Helix-Loop-Helix Proteins; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004594:Electrophysiology; D004622:Embryo, Mammalian; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000494:pharmacology / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D024642:Potassium Channels, Voltage-Gated / Q000502:physiology*; D015222:Sodium Channels / Q000502:physiology; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology*; D013687:Telencephalon / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D014162:Transfection / Q000379:methods
D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; D024642:Potassium Channels, Voltage-Gated; D015222:Sodium Channels; C093564:Neurogenic differentiation factor 1