Species-specific proteins identified in Ascaris lumbricoides and Ascaris suum using two-dimensional electrophoresis.
The protein profile of adult female Ascaris lumbricoides and Ascaris suum originating from humans and pigs, respectively, was studied using two-dimensional polyacrylamide gel electrophoresis. Six different major protein spots specific for A. lumbricoides were identified irrespective of their geographical origin and no major specific spot was encountered in A. suum. No major differences in the protein profiles between the extract by phosphate-buffered saline and urea were encountered for either Ascaris species. It is therefore possible to use 2D-PAGE as a tool for discriminating the closely related Ascaris species from humans and pigs.
Abebe
Wossene
W
Laboratory of Parasitic Diseases, National Institute of Animal Health, National Agricultural Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Tsuji
Naotoshi
N
Kasuga-Aoki
Harue
H
Miyoshi
Takeharu
T
Isobe
Takashi
T
Arakawa
Takeshi
T
Matsumoto
Yasunobu
Y
Yoshihara
Shinobu
S
D000818:Animals; D017164:Ascaris lumbricoides / Q000302:isolation & purification / Q000502:physiology; D017165:Ascaris suum / Q000302:isolation & purification / Q000502:physiology; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D019143:Evolution, Molecular; D005260:Female; D015801:Helminth Proteins / Q000032:analysis*; D006801:Humans; D013045:Species Specificity; D013552:Swine
D015801:Helminth Proteins