title: Experimental study on He-Ne laser irradiation to inhibit scar fibroblast growth in culture.
abstract: OBJECTIVE
  To explore the inhibitory effect of He-Ne laser irradiation on fibroblast growth of hypertrophic scars in culture.
  
  
  METHODS
  He-Ne laser with wavelength of 632.8 nm, power density of 50 mW/cm(2) and doses of 3 J/cm(2), 30 J/cm(2), 90 J/cm(2) and 180 J/cm(2) was used to irradiate human scar fibroblasts in culture 1, 3 and 5 times respectively, and then the cell count and cell cycle analysis were done.
  
  
  RESULTS
  Repeated irradiation with He-Ne laser at dose of 180 J/cm(2) three and five times led to an evident decrease in total cell number compared with that of the control group and there was a significant difference (P<0.05). The cell cycle analysis showed after three and five times of irradiation with 180 J/cm(2) He-Ne laser the cell number in S-phase decreased from 51% to 20% and 14% respectively, the cell number in G(0)/G(1) phase increased from 28% to 55% and 60% respectively, and the cell percentage in Sub-G1 phase was 6.7% and 9.8% respectively.
  
  
  CONCLUSIONS
  Repeated irradiation with 180 J/cm(2) He-Ne laser can inhibit scar fibroblasts growth in culture. It may be that He-Ne laser irradiation causes cell stagnation in G(0)/G(1) phase and apoptosis.
authors.affiliation: Department of Rehabilitation Medicine, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shu
authors.forename: Zongyao
authors.initials: Z
authors.lastname: Wu
authors.forename: Linlin
authors.initials: L
authors.lastname: Hao
authors.forename: Dengfen
authors.initials: D
authors.lastname: Zeng
authors.forename: Guangrui
authors.initials: G
authors.lastname: Feng
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Lin
mesh_terms: D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured; D002921:Cicatrix / Q000473:pathology*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects*; D006371:Helium; D006801:Humans; D007834:Lasers; D008297:Male; D009356:Neon
chemical_list: D006371:Helium; D009356:Neon