Transformation of Neomycin Resistance Gene (neo(R)) into Silkworm (Bombyx mori.L.).
Neomycin resistance gene (neo(R)) flanked by 5' and 3' regions of fibroin H-chain gene of silkworm (Bombyx mori.L.) was transferred into eggs of silkworm by gene gun in the early period of fertilization. The larvae were fed with an artificial diet containing neomycin in early 24 hours post transfettion, and some of them survived. The neo(R) encoding sequence in G(2) generation derived from the survivals was detected by Southern blotting. The results indicated that neo(R) could be used as a selective marker for studies on transgenic silkworm.
Chen
Xiu
X
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. lucd@sunm.shcnc.ac.cn
Zhao
Yun
Y
Zhang
Feng
F
Peng
Wei-Ping
WP
Feng
Xiao-Li
XL
Huang
Jun-Ting
JT
Lu
Chang-De
CD