Tetrakis-acridinyl peptide: a novel fluorometric reagent for nucleic acid analysis based on the fluorescence dequenching upon DNA binding.
Tetrakis-acridinyl peptide 1 assuming a right-handed helical stacked conformation of the acridinyl units can bind to DNA duplex with a very high affinity of 10(8) (M-bp)(-1) even at a high salt concentration of 0.2 M NaCl, irrespective of the base composition. Peptide 1 gave rise to 1600-times enhancement of its fluorescence upon binding to an AT polymer due to the collapse of this stacked structure, but the fluorescence is not enhanced with a GC polymer, though the binding constants of 1 for both polymers were the same.
Ueyama
Hiroyuki
H
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
Takagi
Makoto
M
Takenaka
Shigeori
S
D000677:Amsacrine / Q000031:analogs & derivatives / Q000737:chemistry; D004247:DNA / Q000032:analysis; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D009842:Oligopeptides / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D007364:Intercalating Agents; D009842:Oligopeptides; C466198:tetrakis-acridinyl peptide 1; D000677:Amsacrine; D004247:DNA