Identity between cytolysins purified from two morphos of the Caribbean sea anemone Stichodactyla helianthus.
Stichodactyla helianthus is a sea anemone relatively abundant along Cuban coasts appearing in two morphos with different colors in their tentacles: green or brownish, probably due to their association with algal symbionts. Traditionally, the brownish morpho has been used as a source of sticholysins I and II, the most characterized cytolysins from this anemone, but the green morpho is the most abundant along the western coasts of Havana. The present work is aimed to establish if the cytolysins purified from the green morpho (StIg and StIIg) are similar to those purified from brownish anemones (StI and StII). Following the same chromatographic procedure used to purify the toxins from morphos, the electrophoretic mobilities, amino acid compositions, amino terminal sequences and molecular masses were practically identical between analogal cytolysins. In conclusion, homologous sticholysins purified from the green and brownish variants of Stichodactyla helianthus are the same molecular entities.
Martínez
Diana
D
Department of Biochemistry, Biology Faculty, University of Havana, Calle 25 No 455, Plaza, P.O. Box 10400, Havana City, Cuba.
Morera
Vivian
V
Alvarez
Carlos
C
Tejuca
Mayra
M
Pazos
Fabiola
F
García
Yairet
Y
Raida
Manfred
M
Padrón
Gabriel
G
Eliana Lanio
María
M
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D017691:Caribbean Region; D002851:Chromatography, High Pressure Liquid; D003064:Cnidarian Venoms / Q000737:chemistry* / Q000494:pharmacology; D003603:Cytotoxins / Q000737:chemistry* / Q000494:pharmacology; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry; D012615:Sea Anemones / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D000596:Amino Acids; D003064:Cnidarian Venoms; D003603:Cytotoxins; D010455:Peptides