Amylase hyperproduction by deregulated mutants of the thermophilic fungus Thermomyces lanuginosus.
Thermomyces lanuginosus was subjected to three cycles of mutagenesis (UV/NTG) and a selection procedure to develop amylase-hyperproducing, catabolite-repression-resistant and partially constitutive strains. One of the selected derepressed mutant strain III(51), produced approximately 7- and 3-fold higher specific activity of alpha-amylase (190 U/mg protein) and glucoamylase (105 U/mg protein), respectively, compared to a wild-type parental strain. Further, the effect of production parameters on mutant strain III(51) was studied using a Box-Behnken design. The regression models computed showed significantly high R(2) values of 96 and 97% for alpha-amylase and glucoamylase activities, respectively, indicating that they are appropriate for predicting relationships between corn flour, soybean meal and pH with alpha-amylase and glucoamylase production.
Rubinder
K
K
Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, India.
Chadha
B S
BS
Singh
N
N
Saini
H S
HS
Singh
S
S
D000681:Amylases / Q000096:biosynthesis* / Q000235:genetics; D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis / Q000235:genetics; D016296:Mutagenesis / Q000528:radiation effects; D009154:Mutation / Q000235:genetics* / Q000528:radiation effects; D012641:Selection, Genetic; D014466:Ultraviolet Rays
D000681:Amylases; D005087:Glucan 1,4-alpha-Glucosidase