title: Isolation and characterization of the dcw cluster from the piezophilic deep-sea bacterium Shewanella violacea.
abstract: The dcw cluster of genes involved in cell division and cell wall synthesis from the piezophilic deep-sea bacterium Shewanella violacea was isolated and characterized. It comprises 15 open reading frames, of which the organization is mraZ-mraW-ftsL-ftsI-murE-murF-mraY-murD-ftsW-murG-murC-ftsQ-ftsA-ftsZ-envA, in that order. To analyze transcription upstream from the ftsZ gene, Northern blot and primer extension analyses were performed. The results showed that gene expression is not pressure dependent. Western blot analysis showed that the FtsZ protein is equally expressed under several pressure conditions in the range of atmospheric (0.1 MPa) to high (50 MPa) pressures. Using immunofluorescence microscopy, the FtsZ ring was observed in the center of cells at pressure conditions of 0.1 to 50 MPa. These results imply that the FtsZ protein function is not affected by elevated pressure in this piezophilic bacterium.
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ishii
authors.forename: Kaoru
authors.initials: K
authors.lastname: Nakasone
authors.forename: Takako
authors.initials: T
authors.lastname: Sato
authors.forename: Masaaki
authors.initials: M
authors.lastname: Wachi
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Sugai
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nagai
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kato
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003598:Cytoskeletal Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D011312:Pressure; D016415:Sequence Alignment; D020592:Shewanella / Q000166:cytology / Q000235:genetics* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria