ShiftDetector: detection of shift mutations.
BACKGROUND
Sequencing of a bi-allelic PCR product, which contains an allele with a deletion/insertion mutation results in a superimposed tracefile following the site of this shift mutation. A trace file of this type hampers the use of current computer programs for base calling. ShiftDetector analyses a sequencing trace file in order to discover if it is a superimposed sequence of two molecules that differ in a shift mutation of 1 to 25 bases. The program calculates a probability score for the existence of such a shift and reconstructs the sequence of the original molecule.


BACKGROUND
ShiftDetector is available from http://cowry.agri.huji.ac.il
Seroussi
Eyal
E
Institute of Animal Science, Volcani Center, Bet-Dagan 50250, Israel. seroussi@agri.huji.ac.il
Ron
Micha
M
Kedra
Darek
D
D000483:Alleles; D001483:Base Sequence; D004252:DNA Mutational Analysis / Q000379:methods*; D030541:Databases, Genetic; D015870:Gene Expression; D006580:Genetic Carrier Screening / Q000379:methods*; D020407:Internet; D015233:Models, Statistical; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D009317:National Library of Medicine (U.S.); D016133:Polymerase Chain Reaction / Q000379:methods*; D011381:Programming Languages; D012680:Sensitivity and Specificity; D012984:Software; D014481:United States