Mechanisms of nicotine mediated communication between NGF-differentiated PC12 and HEL cells.
HEL 92.1.7 cells were immobilized among nerve growth factor (NGF)-differentiated PC12 cells. Nicotine caused an immediate Ca2+ mobilisation in the PC12 cells followed by a delayed secondary Ca2+ response in the HEL 92.1.7 cells. The Ca2+ elevation in response to nicotine in PC12 cells was abolished by Na+ removal. The response was diminished by omega-contoxin GVIA (omega-CTx-GVIA) in PC12 cell neurites and by nifedipine in the cell bodies, respectively. The secondary response in HEL 92.1.7 cells was blocked by omega-CTx-GVIA. The results suggest that nicotinic receptor-mediated depolarisation and subsequent activation of voltage dependent Ca2+ channels (VDCC) are sufficient to induce transmitter release from NGF-differentiated PC12 cell varicosities without requirement for additional Ca2+ influx via nicotinic receptor ion channels.
Krjukova
Jelena
J
Division of the Cell Physiology, Department of Physiology, BMC, Uppsala University, Husargatan 3, Sweden.
Akerman
Karl E
KE
D000109:Acetylcholine / Q000378:metabolism; D000313:Adrenal Medulla / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D018679:Cholinergic Agonists / Q000494:pharmacology; D019439:Chromaffin Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008464:Mecamylamine / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018727:Muscarinic Antagonists / Q000494:pharmacology; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D009538:Nicotine / Q000494:pharmacology*; D018733:Nicotinic Antagonists / Q000494:pharmacology; D016716:PC12 Cells; D017729:Presynaptic Terminals / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000378:metabolism*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D018679:Cholinergic Agonists; D018727:Muscarinic Antagonists; D018733:Nicotinic Antagonists; D011978:Receptors, Nicotinic; D008464:Mecamylamine; D009538:Nicotine; D020932:Nerve Growth Factor; D000109:Acetylcholine; D002118:Calcium