Cyanidin 3-O-beta-D-glucoside attenuates the hepatic ischemia-reperfusion injury through a decrease in the neutrophil chemoattractant production in rats.
We have shown that the orally administered cyanidin 3-O-beta-D-glucoside (C3G) attenuates the hepatic ischemia-reperfusion (I/R) injury, which was used as a model for oxidative stress through a decrease in neutrophil chemoattractant production in rats. The rats were subjected to hepatic I/R at 30 min after the administration of C3G (0.9 mmol/kg body weight) or vehicle. I/R treatment resulted in the elevation of oxidative stress marker [liver thiobarbituric acid-reactive substance, Nepsilon-(hexanonyl) lysine and dityrosine] levels in the liver and of the serum activities of marker enzymes for liver injury. The administration of C3G significantly suppressed these elevations, which had been caused by hepatic I/R. Liver myeloperoxidase activity, a useful marker for neutrophil infiltration into tissues, and the plasma and liver concentration of cytokine-induced neutrophil chemoattractant-1 (CINC-1), which has a potent chemotactic activity, were markedly elevated in the control group after hepatic I/R. However, these elevations were significantly suppressed in the C3G group. C3G and its metabolites in the plasma and liver were detected in the C3G group after hepatic I/R. These results suggest that the absorbed C3G and/or its metabolites can act as antioxidants in the blood and liver and scavenge the reactive oxygen species, and brought on a decrease in neutrophil infiltration into the liver through the suppression of CINC-1 production and the tissue damage caused by neutrophils after I/R is attenuated.
Tsuda
Takanori
T
Junior College Division, Tokai Gakuen University, Nagoya, Japan. tsudata@tokaigakuen-c.ac.jp
Horio
Fumihiko
F
Kato
Yoji
Y
Osawa
Toshihiko
T
D000818:Animals; D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers; D002630:Chemotactic Factors / Q000096:biosynthesis*; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D008099:Liver / Q000098:blood supply / Q000201:enzymology; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000872:Anthocyanins; D000975:Antioxidants; D015415:Biomarkers; D002630:Chemotactic Factors; D005960:Glucosides; D017392:Thiobarbituric Acid Reactive Substances; C462279:cyanidin-3-O-beta-glucopyranoside; D009195:Peroxidase