title: Characterization of mouse tGolgin-1 (golgin-245/trans-golgi p230/256 kD golgin) and its upregulation during oligodendrocyte development.
abstract: As part of an effort to identify gene products that are differentially regulated during oligodendrocyte development, we isolated a mouse cDNA that encodes tGolgin-1, a homolog of the human protein known as golgin-245, trans-golgi p230, or 256 kD golgin. Human tGolgin-1 is the target of autoantibodies in patients with Sjögren's syndrome, and is thought to be involved in vesicular transport processes at the trans-Golgi network. Sequencing of cDNAs and EST clones comprising the full-length tGolgin-1 transcript predict marked homology with the amino- and carboxy-terminal regions of the human protein, but more limited homology within the central predicted coiled-coil region. Epitope tagged, truncated forms of mouse tGolgin-1, like those of its human homolog, were localized at steady state to the Golgi/trans-Golgi network in transfected cells. The tGolgin-1 message was expressed in all tissues examined, but was highly upregulated in oligodendrocyte precursors at a stage just prior to myelination. This expression pattern suggests that tGolgin-1 may play a role in specialized transport processes associated with maturation and/or differentiation of oligodendrocyte precursors.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6082, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Cowan
authors.forename: Denise
authors.initials: D
authors.lastname: Gay
authors.forename: Bert M
authors.initials: BM
authors.lastname: Bieler
authors.forename: Huizhen
authors.initials: H
authors.lastname: Zhao
authors.forename: Atsuko
authors.initials: A
authors.lastname: Yoshino
authors.forename: James G
authors.initials: JG
authors.lastname: Davis
authors.forename: Mary M
authors.initials: MM
authors.lastname: Tomayko
authors.forename: Ramachandran
authors.initials: R
authors.lastname: Murali
authors.forename: Mark I
authors.initials: MI
authors.lastname: Greene
authors.forename: Michael S
authors.initials: MS
authors.lastname: Marks
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018507:Gene Expression Regulation, Developmental; D006017:Glycolipids / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D000077614:Golgi Matrix Proteins; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism*; D011510:Proteolipids / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D015854:Up-Regulation
chemical_list: D001324:Autoantigens; D018076:DNA, Complementary; C098772:GOLGA4 protein, human; D006017:Glycolipids; C486294:Golga4 protein, mouse; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; D011510:Proteolipids; D012333:RNA, Messenger