title: Comparative transcription map of the wobbler critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14.
abstract: BACKGROUND
  To support the positional cloning of the mouse mutation wobbler (wr) the corresponding regions on human Chr2p13-14 and mouse Chr11 were analyzed in detail and compared with respect to gene content, order, and orientation.
  
  
  RESULTS
  The gene content of the investigated regions was highly conserved between the two species: 20 orthologous genes were identified on our BAC/YAC contig comprising 4.5 Mb between REL/Rel and RAB1A/Rab1a. Exceptions were pseudogenes ELP and PX19 whose mouse counterparts were not located within the analyzed region. Two independently isolated genomic clones indicate an inversion between man and mouse with the inverted segment being identical to the wobbler critical interval. We investigated the wobbler critical region by extensive STS/EST mapping and genomic sequencing. Additionally, the full-length cDNA sequences of four newly mapped genes as well as the previously mapped gene Otx1 were established and subjected to mutation analysis. Our data indicate that all genes in the wr critical region have been identified.
  
  
  CONCLUSIONS
  Unexpectedly, neither mutation analysis of cDNAs nor levels of mRNAs indicated which of the candidate genes might be affected by the wr mutation. The possibility arises that there might be hitherto unknown effects of mutations, in addition to structural changes of the mRNA or regulatory abnormalities.
authors.affiliation: Developmental Biology and Molecular Pathology, W7, University of Bielefeld, D-33501 Bielefeld, Germany. sonja.fuchs@uni-bielefeld.de
authors.forename: Sonja
authors.initials: S
authors.lastname: Fuchs
authors.forename: Karin
authors.initials: K
authors.lastname: Resch
authors.forename: Cora
authors.initials: C
authors.lastname: Thiel
authors.forename: Michael
authors.initials: M
authors.lastname: Ulbrich
authors.forename: Matthias
authors.initials: M
authors.lastname: Platzer
authors.forename: Harald
authors.initials: H
authors.lastname: Jockusch
authors.forename: Thomas
authors.initials: T
authors.lastname: Schmitt-John
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D020451:Contig Mapping; D003433:Crosses, Genetic; D023061:Gene Order / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants; D020161:Physical Chromosome Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000235:genetics*