title: Lens epithelial cell death after cataract surgery.
abstract: OBJECTIVE
  To determine whether lens epithelial cells (LECs) undergo apoptosis during healing after cataract surgery to further characterize the healing process of the postoperative lens capsule.
  
  
  METHODS
  Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan.
  
  
  METHODS
  Apoptotic cells were detected in human postoperative lens capsules using the terminal deoxynucleotidyl transferase deoxy-UTP nick-end labeling (TUNEL) method. The effect of exogenous transforming growth factor-beta2 (TGF-beta2) on mouse LEC apoptosis was also examined in an organ-culture system.
  
  
  RESULTS
  Three of 17 postoperative specimens contained TUNEL-positive cells. In 2 lens capsules obtained earlier than 10 days, many TUNEL-positive cells, presumably apoptotic LECs, were observed beneath the residual anterior capsule. In cell multilayers in capsule opacification extracted later than 10 days, a few TUNEL-positive cells were seen in 1 specimen; most cells remained negative. In mouse lenses organ-cultured with 1.0 ng/mL TGF-beta2 for 48 hours, TUNEL-positive cells were detected beneath the lens capsule.
  
  
  CONCLUSIONS
  Lens epithelial cells undergo apoptosis during healing after cataract surgery, especially in the early phase. Transforming growth factor-beta2 may be a factor inducing apoptosis in in vivo LECs.
authors.affiliation: Department of Ophthalmology, Wakayama Medical University, 811-1 Kimidera, Wakayama 641-0012, Japan. shizuya@wakayama-med.ac.jp
authors.forename: Shizuya
authors.initials: S
authors.lastname: Saika
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Iku
authors.initials: I
authors.lastname: Ishida
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Akira
authors.initials: A
authors.lastname: Ooshima
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002387:Cataract Extraction; D016923:Cell Death; D004847:Epithelial Cells / Q000502:physiology; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D007903:Lens Capsule, Crystalline / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009924:Organ Culture Techniques; D011184:Postoperative Period; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053781:Transforming Growth Factor beta2; D014945:Wound Healing / Q000502:physiology
chemical_list: C509041:TGFB2 protein, human; D016212:Transforming Growth Factor beta; D053781:Transforming Growth Factor beta2