Structural and biological features of a hydrogel from seed coats of Chorisia speciosa.
Seed coats from Chorisia speciosa form a hydrogel on contact with water. When the hydrogel was solubilized and the solution centrifuged, subsequent ethanol precipitation gave a polysaccharide (F-I) composed of rhamnose, galactose and uronic acid in a molar ratio of 25:44:31. Analysis of F-I by HPSEC-MALLS showed a homogenous polymer with high molecular weight. It consisted of a main chain of (1-->4)-linked beta-galactopyranosyl units as indicated by NMR spectral and methylation data analysis, with rhamnose, galactose and glucuronic acid as non-reducing end units. This fraction interfered with adhesion of Colletotrichum graminicola, a causal agent of anthracnose, to polystyrene slides and to leaves of corn, thus delaying infection in the latter.
Beleski-Carneiro
Eliana
E
Departamento de Bioquímica e Biologie Molecular, Universidade Federal do Paraná. CP 19046, 81531990, Curitiba-PR, Brazil.
Sugui
Janyce
J
Reicher
Fany
F
D002448:Cell Adhesion / Q000187:drug effects; D002850:Chromatography, Gel; D020231:Colletotrichum / Q000187:drug effects / Q000502:physiology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D010944:Plants / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D003313:Zea mays / Q000382:microbiology
D011134:Polysaccharides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate