title: The ferroxidase activity of yeast frataxin.
abstract: Frataxin is required for maintenance of normal mitochondrial iron levels and respiration. The mature form of yeast frataxin (mYfh1p) assembles stepwise into a multimer of 840 kDa (alpha(48)) that accumulates iron in a water-soluble form. Here, two distinct iron oxidation reactions are shown to take place during the initial assembly step (alpha --> alpha(3)). A ferroxidase reaction with a stoichiometry of 2 Fe(II)/O(2) is detected at Fe(II)/mYfh1p ratios of < or = 0.5. Ferroxidation is progressively overcome by autoxidation at Fe(II)/mYfh1p ratios of >0.5. Gel filtration analysis indicates that an oligomer of mYfh1p, alpha(3), is responsible for both reactions. The observed 2 Fe(II)/O(2) stoichiometry implies production of H(2)O(2) during the ferroxidase reaction. However, only a fraction of the expected total H(2)O(2) is detected in solution. Oxidative degradation of mYfh1p during the ferroxidase reaction suggests that most H(2)O(2) reacts with the protein. Accordingly, the addition of mYfh1p to a mixture of Fe(II) and H(2)O(2) results in significant attenuation of Fenton chemistry. Multimer assembly is fully inhibited under anaerobic conditions, indicating that mYfh1p is activated by Fe(II) in the presence of O(2). This combination induces oligomerization and mYfh1p-catalyzed Fe(II) oxidation, starting a process that ultimately leads to the sequestration of as many as 50 Fe(II)/subunit inside the multimer.
authors.affiliation: Department of Pediatric & Adolescent Medicine, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
authors.forename: Sungjo
authors.initials: S
authors.lastname: Park
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Gakh
authors.forename: Steven M
authors.initials: SM
authors.lastname: Mooney
authors.forename: Grazia
authors.initials: G
authors.lastname: Isaya
mesh_terms: D004789:Enzyme Activation; D005656:Fungal Proteins / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D033862:Iron-Binding Proteins / Q000378:metabolism*; D016877:Oxidants / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D000096923:Frataxin
chemical_list: D005656:Fungal Proteins; D006861:Hydrogen Peroxide; D007501:Iron; D033862:Iron-Binding Proteins; D016877:Oxidants; D010088:Oxidoreductases; D010100:Oxygen; D000096923:Frataxin