Chemistry-based studies on oxidative DNA damage: formation, repair, and mutagenesis.
Since the discovery of 8-OH-dG formation, various aspects of oxidative DNA damage have been studied. For example, 2-OH-dA and a glyoxal-dG adduct were discovered as new types of oxidative DNA damage; 2-OH-dATP was found to induce mutations and to be a good substrate of a nucleotide sanitization enzyme, the MTH1 protein; and efforts were continued to establish standard methodologies for 8-OH-dG analyses in urine and cellular DNA. By these studies, we found solid chemistry-based approaches were often useful to clarify the biological phenomena.
Kasai
Hiroshi
H
Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan. h-kasai@med.uoeh-u.ac.jp
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D018736:DNA Adducts / Q000032:analysis; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D005609:Free Radicals; D006801:Humans; D016296:Mutagenesis; D009705:Nucleosides / Q000737:chemistry*; D010084:Oxidation-Reduction
C095141:2-hydroxydeoxyadenosine triphosphate; D001426:Bacterial Proteins; D018736:DNA Adducts; D004269:DNA, Bacterial; D005609:Free Radicals; D009705:Nucleosides; D000080242:8-Hydroxy-2'-Deoxyguanosine; D000255:Adenosine Triphosphate; D004259:DNA-Directed DNA Polymerase; D003849:Deoxyguanosine