title: Regulation of the expression of two female-predominant CYP3A mRNAs (CYP3A41 and CYP3A44) in mouse liver by sex and growth hormones.
abstract: A second female-predominant murine CYP3A, CYP3A44, was isolated from liver and its mRNA expression was compared with that of the previously described CYP3A41. The expression of CYP3A44 was relatively constant after birth in females, whereas it gradually declined in males after 5 weeks of age. The expression of CYP3A41 increased with age in females after 3 weeks of age, whereas it gradually declined in males after 5 weeks of age. Hypophysectomy and growth hormone replacement indicated that expression of both CYP3A mRNAs in females was dependent on the feminine plasma growth hormone profile. Estradiol induced the expression of both mRNAs and the effect was dependent on the presence of the pituitary gland. These observations suggest that endocrine control of expression might be similar, but not identical, for two female-predominant CYP3A mRNAs.
authors.affiliation: Department of Toxicology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan. tsakuma@ms.toyama-mpu.ac.jp
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sakuma
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Endo
authors.forename: Misae
authors.initials: M
authors.lastname: Mashino
authors.forename: Masami
authors.initials: M
authors.lastname: Kuroiwa
authors.forename: Ayako
authors.initials: A
authors.lastname: Ohara
authors.forename: Kanokwan
authors.initials: K
authors.lastname: Jarukamjorn
authors.forename: Nobuo
authors.initials: N
authors.lastname: Nemoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D001483:Base Sequence; D002369:Castration; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D013006:Growth Hormone / Q000494:pharmacology; D007016:Hypophysectomy; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010052:Ovariectomy; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012737:Sex Factors; D013739:Testosterone / Q000494:pharmacology
chemical_list: C464819:Cyp3a44 protein, mouse; D018076:DNA, Complementary; D012333:RNA, Messenger; D013739:Testosterone; D004958:Estradiol; D013006:Growth Hormone; D003577:Cytochrome P-450 Enzyme System; C408695:Cyp3a41a protein, mouse; D010088:Oxidoreductases; D001189:Aryl Hydrocarbon Hydroxylases; D051544:Cytochrome P-450 CYP3A; D010089:Oxidoreductases, N-Demethylating