title: Rafts in adult peripheral nerve myelin contain major structural myelin proteins and myelin and lymphocyte protein (MAL) and CD59 as specific markers.
abstract: The myelin and lymphocyte protein (MAL) proteolipid is localized in central and peripheral compact myelin membranes, as well as in apical membranes of particular polarized cells. In this study, we addressed the question whether MAL and other peripheral myelin proteins are sorted and targeted to myelin membranes using mechanisms similar to those observed in polarized epithelial cells. To investigate the presence of raft-mediated sorting pathways in Schwann cells, we have isolated and analysed their composition in myelin membranes. Here, we show that rafts are present in adult human and rat peripheral compact myelin membranes and contain MAL, the GPI-anchored protein CD59, and substantial amounts of the PMP22 and P0. Colocalization studies show that CD59, and MAL have an almost identical expression pattern within compact myelin. Moreover, immuno-electron microscopy revealed that MAL, besides its localization in compact myelin, is also localized to Schmidt-Lanterman incisures. Taken together, our results demonstrate the presence of detergent-insoluble glycolipid-enriched complexes (DIGs) in different compartments of myelin membranes and indicate an important role for DIG-mediated transport mechanisms in the maintenance of the adult myelin sheath.
authors.affiliation: Department of Research, University Hospital Basel, Pharmacenter, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Erne
authors.forename: S
authors.initials: S
authors.lastname: Sansano
authors.forename: M
authors.initials: M
authors.lastname: Frank
authors.forename: N
authors.initials: N
authors.lastname: Schaeren-Wiemers
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D018957:CD59 Antigens / Q000032:analysis* / Q000096:biosynthesis; D002451:Cell Compartmentation; D003902:Detergents / Q000737:chemistry; D006801:Humans; D046911:Macromolecular Substances; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism; D026901:Membrane Transport Proteins; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D018993:Myelin P0 Protein / Q000032:analysis / Q000096:biosynthesis; D009185:Myelin Proteins / Q000032:analysis* / Q000096:biosynthesis; D009186:Myelin Sheath / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; D010525:Peripheral Nerves / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D011510:Proteolipids / Q000032:analysis* / Q000096:biosynthesis / Q000648:ultrastructure; D051381:Rats; D012995:Solubility; D013126:Spinal Nerve Roots / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D015415:Biomarkers; D018957:CD59 Antigens; D003902:Detergents; C569026:MAL protein, human; D046911:Macromolecular Substances; C569028:Mal protein, rat; D026901:Membrane Transport Proteins; D018993:Myelin P0 Protein; D009185:Myelin Proteins; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; C073687:PMP22 protein, human; C489546:Pmp22 protein, rat; D011510:Proteolipids