title: Isoflurane ameliorates the posthypoxic deoxygenation of the rat brain--the role of cell adhesion molecules of polymorphonuclear leukocytes.
abstract: One of the serious problems that occurs after cardiopulmonary resuscitation is brain posthypoxic/ischemic deoxygenation. However, there has been no report concerning the effect of isoflurane (ISO) on the brain oxygenation during hypoxia-reoxygenation in relation to cell adhesion molecules (CD11b) in polymorphonuclear leukocyte. Rats were anesthetized with a low concentration of ISO (0.5 MAC: low ISO) or high concentration of ISO (1.5 MAC: high ISO) and brain oxygenation was detected by near infrared spectroscopy during 10-min hypoxia (5% O(2)) and a subsequent 120-min reoxygenation period. Hypoxia induced a decrease in oxyhemoglobin (HbO(2)) and an increase in deoxyhemoglobin (Hb). Reoxygenation induced a significant decrease in total hemoglobin (tHb) and HbO(2) with low ISO, but not with high ISO. The changes in Hb were minimal during reoxygenation in both groups. CD11b increased during reoxygenation with low ISO anesthetization, but not with high ISO. A significant negative correlation was observed between CD11b and two of the measured oxyparameters, HbO(2) and tHb, during reoxygenation at low ISO, but not at high ISO. These findings suggest that brain deoxygenation during hypoxia-reoxygenation is partly related to the expression of CD11b. We conclude that ISO modifies the brain circulation at least in part through attenuating the expression of CD11b during hypoxia-reoxygenation.
authors.affiliation: Department of Anesthesiology, Niigata University School of Medicine, Niigata 951-8122, Japan.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Nishimaki
authors.forename: Satoru
authors.initials: S
authors.lastname: Fukuda
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishimoto
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Mori
authors.forename: Hirotake
authors.initials: H
authors.lastname: Eguchi
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Kokubun
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Fujihara
authors.forename: Hisami
authors.initials: H
authors.lastname: Watanabe
authors.forename: Toru
authors.initials: T
authors.lastname: Abo
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000503:physiopathology; D039481:CD11b Antigen / Q000032:analysis / Q000096:biosynthesis; D002560:Cerebrovascular Circulation / Q000187:drug effects; D004569:Electroencephalography; D006439:Hemodynamics; D002534:Hypoxia, Brain / Q000097:blood / Q000517:prevention & control / Q000628:therapy*; D007530:Isoflurane / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D018685:Anesthetics, Inhalation; D039481:CD11b Antigen; D007530:Isoflurane