title: Analysis of HIV-1- and CMV-specific memory CD4 T-cell responses during primary and chronic infection.
abstract: CD4 T-cell-specific memory antiviral responses to human immunodeficiency virus type 1 (HIV-1) and cytomegalovirus (CMV) were investigated in 16 patients with documented primary HIV-1 infection (4 of the 16 subjects also had primary CMV infection) and compared with those observed in patients with chronic HIV-1 and CMV coinfection. Virus-specific memory CD4 T cells were characterized on the basis of the expression of the chemokine receptor CCR7. HIV-1- and CMV-specific interferon-gamma-secreting CD4 T cells were detected in patients with primary and chronic HIV-1 and CMV coinfection and were mostly contained in the cell population lacking expression of CCR7. The magnitude of the primary CMV-specific CD4 T-cell response was significantly greater than that of chronic CMV infection, whereas there were no differences between primary and chronic HIV-1-specific CD4 T-cell responses. A substantial proportion of CD4(+)CCR7(-) T cells were infected with HIV-1. These results advance the characterization of antiviral memory CD4 T-cell response and the delineation of the potential mechanisms that likely prevent the generation of a robust CD4 T-cell immune response during primary infection.
authors.affiliation: Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Harari
authors.forename: G Paolo
authors.initials: GP
authors.lastname: Rizzardi
authors.forename: Kim
authors.initials: K
authors.lastname: Ellefsen
authors.forename: Donatella
authors.initials: D
authors.lastname: Ciuffreda
authors.forename: Patrick
authors.initials: P
authors.lastname: Champagne
authors.forename: Pierre-Alexandre
authors.initials: PA
authors.lastname: Bart
authors.forename: Daniel
authors.initials: D
authors.lastname: Kaufmann
authors.forename: Amalio
authors.initials: A
authors.lastname: Telenti
authors.forename: Roland
authors.initials: R
authors.lastname: Sahli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Tambussi
authors.forename: Laurent
authors.initials: L
authors.lastname: Kaiser
authors.forename: Adriano
authors.initials: A
authors.lastname: Lazzarin
authors.forename: Luc
authors.initials: L
authors.lastname: Perrin
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pantaleo
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000276:immunology*; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002908:Chronic Disease; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000150:complications / Q000276:immunology*; D005260:Female; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D008297:Male; D010641:Phenotype; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000032:analysis; D019562:Viral Load; D014766:Viremia
chemical_list: D000914:Antibodies, Viral; C515370:CCR7 protein, human; D054400:Receptors, CCR7; D019707:Receptors, Chemokine