Unique case of Helicobacter sp. osteomyelitis in an immunocompetent child diagnosed by broad-range 16S PCR.
We report the first case of Helicobacter sp. osteomyelitis in an immunocompetent child. The infection was diagnosed by broad-range 16S PCR followed by sequencing of the resulting amplicon. All other microbiological investigations proved negative. This case highlights the importance of molecular methods in the diagnosis of unsuspected etiological agents and the potential role of Helicobacter sp. in human infection.
Harris
Kathryn A
KA
Department of Microbiology, Great Ormond Street Hospital for Children NHS Trust, London, United Kingdom. harrik2@gosh.nhs.uk
Fidler
Katy J
KJ
Hartley
John C
JC
Vogt
Julie
J
Klein
Nigel J
NJ
Monsell
Fergal
F
Novelli
Vas M
VM
D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D016998:Helicobacter / Q000235:genetics / Q000302:isolation & purification*; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D007121:Immunocompetence; D008969:Molecular Sequence Data; D010019:Osteomyelitis / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S