A role for the octameric ring protein, Translin, in mitotic cell division.
The octameric ring protein, Translin, demonstrates marked similarities to the family of helicase enzymes regarding its quaternary organization and dimerization of subunits. Here we show that the level of Translin closely parallels the proliferative state in various cell types. Expression is periodic during the cell cycle, with protein synthesis becoming maximal in the S and mitosis phases, consistent with a role in cell division. Moreover, induced overexpression of Translin was found to accelerate cell proliferation. Confocal microscopic analysis revealed that Translin is localized at the centrosomes at prophase and the mitotic spindle at metaphase, then translocating to the spindle midbodies during cytokinesis. This novel localization is attributable to specific interactions with microtubules of the mitotic spindles, and especially gamma-tubulin. The results suggest that Translin participates in processes ensuring the segregation of chromosomes and cytokinesis.
Ishida
Reiko
R
Department of Immunology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, 162, Tokyo, Japan.
Okado
Haruo
H
Sato
Hiroko
H
Shionoiri
Chieko
C
Aoki
Katsunori
K
Kasai
Masataka
M
D000818:Animals; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D002455:Cell Division / Q000502:physiology / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D018385:Centrosome / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004318:Doxycycline / Q000494:pharmacology; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis / Q000502:physiology* / Q000528:radiation effects; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D008941:Spindle Apparatus / Q000502:physiology; D014404:Tubulin / Q000378:metabolism
D004268:DNA-Binding Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C095563:TSN protein, human; C498303:Tsn protein, mouse; D014404:Tubulin; D004247:DNA; D001973:Bromodeoxyuridine; D004318:Doxycycline