Efficient delivery of siRNA for inhibition of gene expression in postnatal mice.
It has recently been shown that RNA interference can be induced in cultured mammalian cells by delivery of short interfering RNAs (siRNAs). Here we describe a method for efficient in vivo delivery of siRNAs to organs of postnatal mice and demonstrate effective and specific inhibition of transgene expression in a variety of organs.
Lewis
David L
DL
Mirus Corporation, 505 South Rosa Road, Madison, Wisconsin 53719, USA. davel@genetransfer.com
Hagstrom
James E
JE
Loomis
Aaron G
AG
Wolff
Jon A
JA
Herweijer
Hans
H
D000818:Animals; D000831:Animals, Newborn; D003063:Cnidaria; D001517:Coleoptera; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins; D008099:Liver / Q000378:metabolism; D008156:Luciferases / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D010957:Plasmids; D034741:RNA, Small Interfering; D022661:RNA, Untranslated / Q000008:administration & dosage*; D019076:Transgenes
D008164:Luminescent Proteins; D034741:RNA, Small Interfering; D022661:RNA, Untranslated; D049452:Green Fluorescent Proteins; D008156:Luciferases