2,3,7,8-Tetrachlorodibenzo-p-dioxin is a possible activator of human cytomegalovirus replication in a human fibroblast cell line.
We examined the effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on human cytomegalovirus (CMV) replication in the human embryonic fibroblast cell line, MRC-5. Treatment of cells with 0.0001pg/ml TCDD augmented the cytopathic effects of CMV-infected MRC-5 cells. Moreover, TCDD increased the replication of intracellular CMV without affecting the proliferation of host cells, with a concomitant increase in CMV UL54 DNA levels. However, TCDD demonstrated no virocidal effect on cell-free CMV. Furthermore, CMV-infected MRC-5 cells expressed transcripts of the aryl hydrocarbon receptor (AhR) and AhR nuclear translocator. These results suggest that TCDD may contribute to the development of opportunistic diseases by reactivation of latently infected CMV and that TCDD regulates CMV replication through the dependence on AhR.
Murayama
Tsugiya
T
Division of Persistent and Oncogenic Viruses, Center for Chronic Viral Diseases, Faculty of Medicine, Kagoshima University, Kagoshima 890-8520, Japan. t-mura@m3.kufm.kagoshima-u.ac.jp
Inoue
Masao
M
Nomura
Taisei
T
Mori
Seiichiro
S
Eizuru
Yoshito
Y
D002460:Cell Line; D003587:Cytomegalovirus / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000821:virology*; D006801:Humans; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology*; D018336:Receptors, Aryl Hydrocarbon / Q000502:physiology; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000187:drug effects*
D004279:DNA, Viral; D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon