Experimental protein mixture for validating tandem mass spectral analysis.
Several methods have been used to identify peptides that correspond to tandem mass spectra. In this work, we describe a data set of low energy tandem mass spectra generated from a control mixture of known protein components that can be used to evaluate the accuracy of these methods. As an example, these spectra were searched by the SEQUEST application against a human peptide sequence database. The numbers of resulting correct and incorrect peptide assignments were then determined. We show how the sensitivity and error rate are affected by the use of various filtering criteria based upon SEQUEST scores and the number of tryptic termini of assigned peptides.
Keller
Andrew
A
Institute for Systems Biology, Seattle, Washington 98103, USA. akeller@systemsbiology.org
Purvine
Samuel
S
Nesvizhskii
Alexey I
AI
Stolyar
Sergey
S
Goodlett
David R
DR
Kolker
Eugene
E
D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012984:Software
D010455:Peptides