Molecular cloning and expression analysis of MPP alpha-2, a novel mouse transcript detected in a differential screen of pituitary libraries.
We identified a novel isoform transcript, MPP alpha-2, of the mouse Mg(2+)-dependent protein phosphatase (MPP) alpha gene. The amino acid sequence encoded by MPP alpha-2 differs from the previously known MPP alpha-1 sequence only at the carboxyl terminal region. Northern and in situ hybridization analysis revealed differential expression patterns of these two transcripts in the embryo and in the adult organism, suggesting an elaborate regulation of the MPP alpha gene.
Miyamoto
Toshinobu
T
Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Fiorenza
Maria T
MT
Zhao
Yangu
Y
Hasuike
Shiga
S
Westphal
Heiner
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D015723:Gene Library; D051379:Mice; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis* / Q000235:genetics*; D010902:Pituitary Gland / Q000196:embryology / Q000201:enzymology*; D000071636:Protein Phosphatase 2C; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014158:Transcription, Genetic
D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C