title: Restraint stress alters the expression of interleukin-1 and keratinocyte growth factor at the wound site: an in situ hybridization study.
abstract: Stress has a negative impact on wound healing. This murine study evaluated the effect of restraint stress (RST) on interleukin-1 (IL-1) and keratinocyte growth factor-1 (KGF-1) gene expression in cutaneous wounds by in situ hybridization. At day 1, RST mice had reduced frequency of IL-1beta mRNA-expressing fibroblasts compared to control mice. At day 3, RST mice had reduced frequencies of IL-1beta mRNA-expressing leukocytes, KGF-1 mRNA-expressing fibroblasts, and thin proliferating zones of epithelium. At day 5, RST mice had persistent leukocytic foci and reduced granulation tissue. Therefore, stress-induced alterations in cutaneous proinflammatory cytokine and growth factor expression are associated with significant histological changes in healing wounds.
authors.affiliation: Department of Oral Biology, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Mercado
authors.forename: Ning
authors.initials: N
authors.lastname: Quan
authors.forename: David A
authors.initials: DA
authors.lastname: Padgett
authors.forename: John F
authors.initials: JF
authors.lastname: Sheridan
authors.forename: Phillip T
authors.initials: PT
authors.lastname: Marucha
mesh_terms: D000818:Animals; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D005260:Female; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors / Q000235:genetics*; D005347:Fibroblasts / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology; D017403:In Situ Hybridization; D007249:Inflammation / Q000235:genetics* / Q000276:immunology / Q000503:physiopathology; D007375:Interleukin-1 / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000276:immunology / Q000378:metabolism; D012149:Restraint, Physical; D012867:Skin / Q000276:immunology* / Q000293:injuries / Q000503:physiopathology; D013312:Stress, Physiological / Q000235:genetics* / Q000276:immunology / Q000503:physiopathology; D014945:Wound Healing / Q000235:genetics*
chemical_list: C496454:Fgf7 protein, mouse; D007375:Interleukin-1; D012333:RNA, Messenger; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors