title: Crystal structure of recombinant human interleukin-22.
abstract: Interleukin-22 (IL-10-related T cell-derived inducible factor/IL-TIF/IL-22) is a novel cytokine belonging to the IL-10 family. Recombinant human IL-22 (hIL-22) was found to activate the signal transducers and activators of transcription factors 1 and 3 as well as acute phase reactants in several hepatoma cell lines, suggesting its involvement in the inflammatory response. The crystallographic structure of recombinant hIL-22 has been solved at 2.0 A resolution using the SIRAS method. Contrary to IL-10, the hIL-22 dimer does not present an interpenetration of the secondary-structure elements belonging to the two distinct polypeptide chains but results from interface interactions between monomers. Structural differences between these two cytokines, revealed by the crystallographic studies, clearly indicate that, while a homodimer of IL-10 is required for signaling, hIL-22 most probably interacts with its receptor as a monomer.
authors.affiliation: Laboratório Nacional de Luz Síncrotron, São Paulo, Brazil.
authors.forename: Ronaldo Alves Pinto
authors.initials: RA
authors.lastname: Nagem
authors.forename: Didier
authors.initials: D
authors.lastname: Colau
authors.forename: Laure
authors.initials: L
authors.lastname: Dumoutier
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Renauld
authors.forename: Craig
authors.initials: C
authors.lastname: Ogata
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018360:Crystallography, X-Ray; D019281:Dimerization; D004220:Disulfides / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000737:chemistry / Q000235:genetics; D016753:Interleukin-10 / Q000737:chemistry / Q000235:genetics; D007378:Interleukins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D018123:Receptors, Interleukin / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D000097043:Interleukin-22
chemical_list: D004220:Disulfides; D007371:Interferon-gamma; D016753:Interleukin-10; D007378:Interleukins; D018123:Receptors, Interleukin; D011994:Recombinant Proteins; D000097043:Interleukin-22