title: Thrombospondin-1 mediates smooth muscle cell proliferation induced by interaction with human platelets.
abstract: OBJECTIVE
  Platelet adherence and activation are associated with smooth muscle cell (SMC) proliferation and arterial restenosis. This study examined platelet-SMC interaction on fibrillar type I collagen and analyzed the role of thrombospondin (TSP)-1 in platelet-induced SMC proliferation.
  
  
  RESULTS
  When SMCs cultured on fibrillar collagen were treated with human platelets (5 preparations), 7.45+/-2.94% of the cells passed through S phase within 24 hours, as determined by bromodeoxyuridine nuclear labeling. The addition of platelets markedly induced SMC TSP-1 mRNA expression and cell surface protein accumulation, which colocalized with adhered platelets, as determined by alpha(IIb) integrin immunostaining. Direct interaction of platelets with SMCs was necessary for its effect on proliferation and TSP-1 accumulation, as determined in the transwell culture system. The anti-TSP-1 blocking antibody strongly inhibited platelet-induced SMC proliferation by approximately 60%. Analysis of the receptors for TSP-1 accumulation on the SMC surface revealed that beta1 integrins are mainly involved. The anti-beta1 integrin blocking antibody, which potently suppressed TSP-1 accumulation on SMCs, also markedly inhibited platelet-stimulated SMC proliferation.
  
  
  CONCLUSIONS
  TSP-1 and beta1 integrin interaction is involved in platelet-stimulated SMC proliferation. This in vitro coculture system could prove useful for examining the molecular mechanism underlying platelet-induced vascular remodeling and for studying the mechanism of a tested drug for restenosis.
authors.affiliation: Department of Biochemistry, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Ichii
authors.forename: Hidenori
authors.initials: H
authors.lastname: Koyama
authors.forename: Shinji
authors.initials: S
authors.lastname: Tanaka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shioi
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Okuno
authors.forename: Shuzo
authors.initials: S
authors.lastname: Otani
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Nishizawa
mesh_terms: D001161:Arteriosclerosis / Q000378:metabolism / Q000473:pathology; D001792:Blood Platelets / Q000502:physiology*; D002455:Cell Division; D002478:Cells, Cultured; D018920:Coculture Techniques; D024022:Fibrillar Collagens; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D012008:Recurrence; D019700:Thrombospondin 1 / Q000378:metabolism*
chemical_list: D024022:Fibrillar Collagens; D019012:Integrin beta1; D019700:Thrombospondin 1