title: Evidence for the preservation of specific intermolecular interactions in gaseous protein-oligosaccharide complexes.
abstract: Arrhenius parameters, obtained with the blackbody infrared radiative dissociation technique, are reported for the dissociation of a series of gaseous protonated complexes composed of mutants of a single chain variable fragment (scFv) of the monoclonal antibody Se155-4 and three trisaccharide ligands. Hydrogen bonding between a ligand and a particular amino acid residue is identified from a comparison of activation energies measured for the complex of the unmodified scFv and the corresponding mutant. It is shown that the specific hydrogen bond between His(101H) and Man C-4 OH is preserved in the gaseous complex.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Canada T6G 2G2.
authors.forename: Elena N
authors.initials: EN
authors.lastname: Kitova
authors.forename: David R
authors.initials: DR
authors.lastname: Bundle
authors.forename: John S
authors.initials: JS
authors.lastname: Klassen
mesh_terms: D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; D011506:Proteins