Population analysis of CD4+ T cell chemokine receptor transcript expression during in vivo type-1 (mycobacterial) and type-2 (schistosomal) immune responses.
Chemokine receptor transcripts were defined among CD4+ T cells in lymph nodes of mice with type-1 and type-2 inflammation, respectively, elicited by mycobacterial and schistosomal Ag. CXCR3 and CCR6 transcripts were biased to type-1, and CCR4 transcripts increased in type-1 and type-2 populations. CCR3 and CCR5 signals were too weak to establish differences. CCR8 transcripts were not increased among unstimulated populations. Compared to naïve, type-1 and type-2 populations had reduced CCR7 and enhanced CXCR5 transcripts, consistent with a shift to memory cells. Subset depletion revealed that transcript expression was induced among CD44+ memory T cells. Surprisingly, CCR3 transcripts were enriched among CD44lo fractions. Ag stimulation augmented CXCR3, CCR4, and CCR8 but down-regulated CCR6 and CXCR5. CCR4 showed association with IFN-gamma- and IL-4-producing cells, but other receptor transcripts were expressed among IFN-gamma/IL-4 negative memory T cells. These studies provide several novel findings regarding Th cell chemokine receptor expression in vivo.
Chiu
Bo-Chin
BC
Department of Pathology, University of Michigan Medical School, Ann Arbor, USA.
Shang
Xiao-Zhou
XZ
Stolberg
Valerie R
VR
Komuniecki
Eric
E
Chensue
Stephen W
SW
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008808:Mice, Inbred CBA; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D054397:Receptors, CCR3; D054398:Receptors, CCR4; D054399:Receptors, CCR6; D054400:Receptors, CCR7; D054401:Receptors, CCR8; D054367:Receptors, CXCR3; D054380:Receptors, CXCR5; D019707:Receptors, Chemokine / Q000096:biosynthesis* / Q000235:genetics; D018121:Receptors, Cytokine / Q000096:biosynthesis / Q000235:genetics; D012550:Schistosoma mansoni / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism*; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism*; D014373:Tuberculin / Q000276:immunology*
D000942:Antigens, Bacterial; D000953:Antigens, Protozoan; C515350:CCR3 protein, human; C515354:CCR4 protein, human; C106311:CCR6 protein, human; C515370:CCR7 protein, human; C103330:CCR8 protein, human; C515187:CXCR3 protein, human; C513179:CXCR5 protein, mouse; C515351:Ccr3 protein, mouse; C515355:Ccr4 protein, mouse; C515371:Ccr7 protein, mouse; C515188:Cxcr3 protein, mouse; D012333:RNA, Messenger; D054397:Receptors, CCR3; D054398:Receptors, CCR4; D054399:Receptors, CCR6; D054400:Receptors, CCR7; D054401:Receptors, CCR8; D054367:Receptors, CXCR3; D054380:Receptors, CXCR5; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D014373:Tuberculin; D015847:Interleukin-4; D007371:Interferon-gamma