title: Adrenergic nerve fibres and mast cells: correlation in rat thymus.
abstract: The interactions between adrenergic nerve fibres and mast cells (MCs) were studied in the thymus of adult and old rats by morphological methods and by quantitative analysis of images (QAIs). The whole thymus was drawn in adult (12 months old) rats: normal, sympathectomized or electrostimulated. Thymuses from the above-mentioned animals were weighed, measured and dissected. Thymic slices were stained with eosin orange for detection of microanatomical details and with Bodian's method for identification of the whole nerve fibres. Thymic MCs were stained with Astrablau. Histofluorescence microscopy was used for staining of adrenergic nerve fibres. Finally, all morphological results were submitted to the QAIs and statistical analysis of data. Our results suggest that after surgical sympathectomy, the greater part of adrenergic nerve fibres disappear while related MCs appear to show less evident fluorescence and few granules. On the contrary, electrostimulation of the cervical superior ganglion induced an increase in the fluorescence of adrenergic nerve fibres and of related MCs.
authors.affiliation: Department of Pharmacology of Natural Molecules and General Physiology, University of Rome 'La Sapienza', P le A. Molo 5, Rome, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Artico
authors.forename: Carlo
authors.initials: C
authors.lastname: Cavallotti
authors.forename: Daniela
authors.initials: D
authors.lastname: Cavallotti
mesh_terms: D000320:Adrenergic Fibers / Q000276:immunology / Q000502:physiology*; D000818:Animals; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D008856:Microscopy, Fluorescence; D015213:Neuroimmunomodulation; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology / Q000294:innervation*