Parameters related to oxygen free radicals in erythrocytes, plasma and epidermis of the hairless rat.
The following parameters related to oxygen free radicals (OFR) were determined in erythrocytes and the epidermis of hairless rats: catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), reduced (GSH) and oxidized (GSSG) glutathione, glutathione S-transferase (GST), superoxide dismutase (SOD) and thiobarbituric acid reactive substances (TBARS). GSH, GSSG and TBARS were also analyzed in plasma. In erythrocytes, the Pearson correlation coefficients (r) were significant (p < 0.001) between glutathione and other parameters as follows: GSH correlated negatively with GSSG (r = -0.665) and TBARS (r = -0.669); GSSG correlated positively with SOD (r = 0.709) and TBARS (r = 0.752). Plasma GSSG correlated negatively with erythrocytic thermostable GST activity (r = -0.608; p=0.001) and with erythrocytic total GST activity (r = -0.677; p < 0.001). In epidermis (p < 0.001 in all cases), GSH content correlated with GSSG (r = 0.682) and with GPx (r = 0.663); GSSG correlated with GPx (r = 0.731) and with GR (r = 0.794). By multiple linear regression analysis some predictor variables (R(2)) were found: in erythrocytes, thermostable GST was predicted by total GST activity and GSSG, GSSG content was predicted by GSH and by the GSH/GSSG ratio and GPx activity was predicted by GST, CAT and SOD activities; in epidermis, GSSG was predicted by GR and SOD activities and GR was predicted by GSSG, TBARS and GPx. It is concluded that the hairless rat is a good model for studying OFR-related parameters simultaneously in blood and skin, and that it may provide valuable information about other animals under oxidative stress.
Romeu
M
M
School of Medicine, University Rovira i Virgili, Sant Llorenç, 21, 43201-REUS, Spain.
Mulero
M
M
Giralt
M
M
Folch
J
J
Nogués
M R
MR
Torres
A
A
Fortuño
A
A
Sureda
F X
FX
Cabré
M
M
Paternáin
J L
JL
Mallol
J
J
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002374:Catalase / Q000097:blood / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005609:Free Radicals / Q000097:blood / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000097:blood / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D010100:Oxygen / Q000097:blood / Q000378:metabolism*; D010949:Plasma / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D011923:Rats, Nude; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D001798:Blood Proteins; D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D010100:Oxygen