title: Experimental protein mixture for validating tandem mass spectral analysis.
abstract: Several methods have been used to identify peptides that correspond to tandem mass spectra. In this work, we describe a data set of low energy tandem mass spectra generated from a control mixture of known protein components that can be used to evaluate the accuracy of these methods. As an example, these spectra were searched by the SEQUEST application against a human peptide sequence database. The numbers of resulting correct and incorrect peptide assignments were then determined. We show how the sensitivity and error rate are affected by the use of various filtering criteria based upon SEQUEST scores and the number of tryptic termini of assigned peptides.
authors.affiliation: Institute for Systems Biology, Seattle, Washington 98103, USA. akeller@systemsbiology.org
authors.forename: Andrew
authors.initials: A
authors.lastname: Keller
authors.forename: Samuel
authors.initials: S
authors.lastname: Purvine
authors.forename: Alexey I
authors.initials: AI
authors.lastname: Nesvizhskii
authors.forename: Sergey
authors.initials: S
authors.lastname: Stolyar
authors.forename: David R
authors.initials: DR
authors.lastname: Goodlett
authors.forename: Eugene
authors.initials: E
authors.lastname: Kolker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012984:Software
chemical_list: D010455:Peptides