title: Analysis of C60 and C70 fullerenes using high-performance liquid chromatography-fourier transform infrared spectroscopy.
abstract: The performance of Fourier transform infrared spectroscopy (FT-IR) detection coupled to high-performance liquid chromatography for the analysis of C60 and C70 fullerenes was investigated. The isocratic separation method involved an octadecylsilane (ODS) column and an acetonitrile-toluene (1:1) mobile phase. The hyphenated system was designed with a split valve to control eluent volume leading to the FT-IR detector; this allowed for additional coupling of the liquid chromatograph to ultraviolet-visible detection. On-line FT-IR spectra of C60 and C70 were matched with standard off-line FT-IR spectra from the literature. In addition, with band chromatograms individual fullerenes can be identified using FT-IR active modes known specifically for each fullerene. Few changes to a pre-existing HPLC-UV method were necessary for the HPLC-FT-IR method, and there was no need for fraction collection to identify the fullerenes C60 and C70.
authors.affiliation: Chemistry Department, University of Rhode Island, Kingston 02881, USA.
authors.forename: James M
authors.initials: JM
authors.lastname: Treubig
authors.forename: Phyllis R
authors.initials: PR
authors.lastname: Brown
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D037741:Fullerenes / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D037741:Fullerenes; C069836:fullerene C70; C069837:fullerene C60