Crystallisation of membrane proteins mediated by antibody fragments.
X-ray structures of three different membrane proteins in complex with antibody fragments have been published. The binding of Fv or Fab fragments enlarges the hydrophilic part of integral membrane proteins, thereby providing additional surface for crystal contacts and space for the detergent micelle. In all reported cases, antibody binding was either essential for the crystallisation of the membrane protein or it substantially improved the diffraction quality of the crystals. Antibody-fragment-mediated crystallisation appears to be a valuable tool in particular for membrane proteins with very small hydrophilic or flexible domains.
Hunte
Carola
C
Max-Planck-Institut für Biophysik, Abt Molekulare Membranbiologie, Heinrich-Hoffmann-Strasse 7, D-60528 Frankfurt am Main, Germany. carola.hunte@mpibp-frankfurt.mpg.de
Michel
Hartmut
H
D000911:Antibodies, Monoclonal / Q000737:chemistry; D003460:Crystallization / Q000379:methods* / Q000639:trends; D003461:Crystallography / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D014450:Electron Transport Complex III / Q000737:chemistry; D003576:Electron Transport Complex IV / Q000737:chemistry; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D010231:Paracoccus denitrificans / Q000201:enzymology; D015221:Potassium Channels / Q000737:chemistry; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry
D000911:Antibodies, Monoclonal; D007128:Immunoglobulin Fragments; D008565:Membrane Proteins; D015221:Potassium Channels; D011994:Recombinant Proteins; D003576:Electron Transport Complex IV; D014450:Electron Transport Complex III