Expression of DNA topoisomerase IIalpha in oligodendroglioma.
BACKGROUND
DNA Topoisomerase ha (Topo flu) is a nuclear enzyme whose expression levels are related to tumor cell growth and to resistance to anticancer chemotherapy.


METHODS
Topo ha expression was assessed immunohistochemically in 60 surgical specimens of 47 consecutive oligodendroglioma patients. Expression levels were correlated with the MIB-1 proliferation index and with patient outcome.


RESULTS
Topo flu expression ranges from 0.3 to 40.1% and is significantly associated with MIB-) staining. There is a weak trend to a higher expression of Topo flu in anaplastic oligodendrogliomas (p = 0.08). Topo ha expression is not related to patient outcome.


CONCLUSIONS
Topo flu expression appears to serve as a marker for tumor growth in human oligodendrogliomas. The widely vaiying expression levels of Topo ha in oligodendroglioma suggest this enzyme could be used for targeted selection of patients for Topo flu-interfering chemotherapy.
Bredel
Markus
M
Institute of Neurology, Department of Neurosurgery, University of Vienna, Wien, Austria.
Gatterbauer
Brigitte
B
Birner
Peter
P
Haberler
Christine
C
Ströbel
Thomas
T
Budka
Herbert
H
Rössler
Karl
K
Hainfellner
Johannes A
JA
D000293:Adolescent; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm; D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004250:DNA Topoisomerases, Type II / Q000096:biosynthesis*; D004268:DNA-Binding Proteins; D006801:Humans; D008875:Middle Aged; D009837:Oligodendroglioma / Q000201:enzymology* / Q000473:pathology; D012189:Retrospective Studies
D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D004250:DNA Topoisomerases, Type II