pH effects on measurements of ionized calcium and ionized magnesium in blood.
BACKGROUND
It is well known that the concentration of ionized calcium in blood is affected by the pH of the specimen, since hydrogen ions compete with calcium for binding sites on albumin and other proteins. However, the relationship between pH and ionized magnesium concentration is not as well characterized.


OBJECTIVE
To determine the effects of pH on ionized magnesium concentration over a wide range of pH values in serum or plasma.


METHODS
Both ionized calcium and ionized magnesium concentrations were measured in 3 sets of samples. (1) Pools of serum or whole blood at different pH values (7.20-7.60) achieved by adding a constant volume of acid or base (diluted solutions of either hydrochloric acid or sodium hydroxide) plus saline. These pools consisted of 2 serum and 3 heparinized whole blood pools collected from leftover blood remaining in clinical specimens in the Clinical Chemistry and Blood Gas Laboratories, respectively, at Duke University Medical Center. (2) Five whole blood specimens obtained from apparently healthy individual donors. (3) Twenty-six whole blood specimens obtained from individual patients (leftover blood from the Blood Gas Laboratory) in which pH was varied by in vitro loss or gain of carbon dioxide.


RESULTS
Both ionized calcium and ionized magnesium concentrations decreased as the pH in the specimen increased, indicating the stronger binding of these ions with proteins in the more alkaline environment.


CONCLUSIONS
We conclude that the rate of change of ionized magnesium concentration with pH change (0.12 mmol/L per pH unit) is significantly less than that of ionized calcium (0.36 mmol/L per pH unit). Furthermore, our findings indicate that if adjustment to pH 7.40 is necessary, the ionized magnesium test results need to be adjusted when pH is markedly abnormal, as is sometimes done for ionized calcium.
Wang
Sihe
S
Department of Pathology/Clinical Laboratories, Duke University Medical Center, Durham, NC 27710, USA.
McDonnell
Elizabeth H
EH
Sedor
Frank A
FA
Toffaletti
John G
JG
D002118:Calcium / Q000097:blood*; D002245:Carbon Dioxide / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000097:blood*; D012709:Serum Albumin / Q000032:analysis
D012709:Serum Albumin; D002245:Carbon Dioxide; D008274:Magnesium; D002118:Calcium