Adaptation of the white-rot basidiomycete Panus tigrinus for transformation of high concentrations of chlorophenols.
During feed-batch cultivation of the white-rot fungus Panus tigrinus in a 5-l bioreactor on N-limited medium, 100, 200, 500, 1,000 and 2,000 mg 2,4,6-trichlorophenol (2,4,6-TCP) l(-1) were added sequentially after 90% removal of the previous portion of the toxicant. The addition of 500 mg 2,4,6-TCP l(-1) without preliminary adaptation killed the culture. The addition of 300 mg 2,4,6-TCP l(-1) without prior adaptation resulted in its slower removal than removal of 2,000 mg 2,4,6-TCP l(-1) by this adapted culture. After adaptation of P. tigrinus to 2,4,6-TCP in a 72-l bioreactor, the mixture of 2,4-dichlorophenol, 2,4,6-TCP, and pentachlorophenol, each at 500 mg x l(-1), was totally removed over 3 weeks. No lignin peroxidase activity was found in the course of cultivation of the fungus. Laccase activity was suppressed by addition of 2,4,6-TCP. Mn-peroxidase was found to be responsible for transformation of the chlorophenols. As final products of the process, several newly formed aromatic polymers, both chlorinated and non-chlorinated, were found in the culture liquid.
Leontievsky
A A
AA
Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences (IBPM RAS), 142290 Pushchino Moscow region, Prospekt Nauki 5, Russia. leont@ibpm.serpukhov.su
Myasoedova
N M
NM
Golovleva
L A
LA
Sedarati
M
M
Evans
C S
CS
D000222:Adaptation, Physiological; D001487:Basidiomycota / Q000254:growth & development* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002733:Chlorophenols / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D010544:Peroxidases / Q000378:metabolism
D002733:Chlorophenols; D003470:Culture Media; D010544:Peroxidases; C051129:manganese peroxidase; C024564:2,4,6-trichlorophenol