title: Biophysical evidence of lipid and carbohydrate binding activities of shrimp high density lipoprotein/beta glucan binding protein.
abstract: Crustacean High Density Lipoprotein/beta-Glucan Binding Protein (HDL/BGBP) has been studied due to its role in nutrition and immune response via activation of the defense cells (hemocytes) upon binding 1,3-D-beta-glucan carbohydrates. In this study, HDL/BGBP was found to be composed mainly of beta sheets, as determined by circular dichroism. Lipoprotein aggregation resulted when HDL/BGBP interacted with phospolipid vesicles, laminaribiose (1,3-beta-glucan disaccharide) or heparin. HDL/BGBP has similar dissociation constants for laminaribiose (K(d)=22 mM) or heparin (K(d)=46 mM) as determined by 90 degrees light scattering.
authors.affiliation: Marine Biotechnology Laboratory, Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo, Sonora, 83000, México.
authors.forename: Claudia L
authors.initials: CL
authors.lastname: Vargas-Requena
authors.forename: Alejandra
authors.initials: A
authors.lastname: Hernández-Santoyo
authors.forename: Gloria
authors.initials: G
authors.lastname: Yepiz-Plascencia
authors.forename: Francisco
authors.initials: F
authors.lastname: Vargas-Albores
authors.forename: Inocencio
authors.initials: I
authors.lastname: Higuera-Ciapara
authors.forename: Adela
authors.initials: A
authors.lastname: Rodríguez-Romero
authors.forename: José L
authors.initials: JL
authors.lastname: Soulages
authors.forename: Rogerio R
authors.initials: RR
authors.lastname: Sotelo-Mundo
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D033364:Decapoda / Q000737:chemistry / Q000502:physiology*; D006458:Hemolymph / Q000737:chemistry; D006493:Heparin / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D017433:Protein Structure, Secondary
chemical_list: D002352:Carrier Proteins; D008077:Lipoproteins, LDL; D008081:Liposomes; D006493:Heparin