Preparation of a subcellular conjugate with the lipopolysaccharide from Vibrio cholerae 01 using beta-D-glucan as matrix.
A conjugate consisting of detoxified lipopolysaccharide of Vibrio cholerae, a carrier polysaccharide matrix and an immunogenic protein has been synthesised and the reaction conditions have been optimised for obtaining a high degree of conjugation. The obtained construct showed reactivity with the antibodies against V. cholerae and can serve as a prospective candidate for preparation of subcellular anti-cholera vaccine.
Machová
Eva
E
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 842 38 Bratislava, Slovakia. chememch@savba.sk
Bystrický
Slavomír
S
Gáliková
Anna
A
Kogan
Grigorij
G
D000276:Adjuvants, Immunologic / Q000737:chemistry; D000688:Amylose / Q000737:chemistry / Q000276:immunology; D000907:Antibodies, Bacterial / Q000276:immunology; D002240:Carbohydrate Sequence; D005936:Glucans / Q000737:chemistry / Q000276:immunology; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry* / Q000276:immunology; D005779:Immunodiffusion; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D012709:Serum Albumin / Q000737:chemistry / Q000276:immunology; D014612:Vaccines; D041241:Vibrio cholerae O1 / Q000737:chemistry* / Q000276:immunology; D015003:Yeasts / Q000737:chemistry
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D005936:Glucans; D018796:Immunoconjugates; D008070:Lipopolysaccharides; D012709:Serum Albumin; D014612:Vaccines; D000688:Amylose