High metabolic function of primary human and porcine hepatocytes in a polyurethane foam/spheroid culture system in plasma from patients with fulminant hepatic failure.
It has been demonstrated that plasma from patients with fulminant hepatic failure (FHF) interferes extensively with cellular function. We placed primary human and primary porcine hepatocytes in a polyurethane foam (PUF)/spheroid culture system and compared the metabolic functions in the plasma of patients with FHF in a 24-h stationary culture to those in a monolayer culture. The PUF/spheroid culture system using primary human and primary porcine hepatocytes significantly decreased ammonia content during 28-day culture. Fisher's ratio significantly increased at culture days 3 and 7. Tauroursodeoxycholic acid significantly increased and glycochenodeoxycholic acid and taurochenodeoxycholic acid decreased in the FHF patients' plasma at culture day 3. During at least a 24-h culture in the FHF patients' plasma, metabolic functions of primary human and primary porcine hepatocytes were almost identical. The present results indicate that the PUF/spheroid culture system using primary human or primary porcine hepatocytes demonstrated more advantageous metabolic functions in the plasma from patients with FHF than the monolayer culture.
Yamashita
Yo-ichi
Y
Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. yamashi@surg2.med.kyushu-u.ac.jp
Shimada
Mitsuo
M
Tsujita
Eiji
E
Shirabe
Ken
K
Ijima
Hiroyuki
H
Nakazawa
Kohji
K
Sakiyama
Ryoichi
R
Fukuda
Junji
J
Funatsu
Kazumori
K
Sugimachi
Keizo
K
D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D005999:Glycochenodeoxycholic Acid / Q000097:blood / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D017093:Liver Failure / Q000097:blood* / Q000378:metabolism*; D011140:Polyurethanes / Q000378:metabolism*; D018874:Spheroids, Cellular / Q000378:metabolism*; D013552:Swine; D013655:Taurochenodeoxycholic Acid / Q000097:blood / Q000378:metabolism; D013997:Time Factors
D001647:Bile Acids and Salts; D011140:Polyurethanes; D013655:Taurochenodeoxycholic Acid; C031655:ursodoxicoltaurine; D005999:Glycochenodeoxycholic Acid; D000641:Ammonia; C028279:polyurethane foam