Detection of molecules related to the GABAergic system in a single-cell eukaryote, Paramecium primaurelia.
Gamma-aminobutyric acid (GABA)-related molecules were identified in Paramecium primaurelia by immunocytochemical methods, and GABA(A) receptors by their histochemical BODIPY-binding sites. Confocal microscope analysis showed different localizations according to the stages of the developmental cycle. A comparison was made with the cholinergic molecules, such as the acetylcholine biosynthetic enzyme (choline acetyltransferase), in double-labelled cells by confocal microscopy. In vivo experiments suggested the involvement of GABA-related molecules in cell-cell interaction.
Delmonte Corrado
Maria U
MU
Department for the Study of the Territory and its Resources, University of Genoa, Corso Europa 26, 16132 Genoa, Italy. corrado@dipteris.unige.it
Ognibene
Marzia
M
Trielli
Francesca
F
Politi
Huguette
H
Passalacqua
Mario
M
Falugi
Carla
C
D000612:4-Aminobutyrate Transaminase / Q000032:analysis / Q000276:immunology / Q000494:pharmacology; D000109:Acetylcholine / Q000502:physiology; D000445:Aldehyde Oxidoreductases / Q000032:analysis / Q000276:immunology / Q000494:pharmacology; D000818:Animals; D000906:Antibodies; D001640:Bicuculline / Q000494:pharmacology; D001896:Boron Compounds; D002795:Choline O-Acetyltransferase / Q000032:analysis / Q000276:immunology; D005456:Fluorescent Dyes; D018755:GABA Agonists / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D007150:Immunohistochemistry; D009118:Muscimol / Q000494:pharmacology; D010247:Paramecium / Q000737:chemistry* / Q000187:drug effects; D010852:Picrotoxin / Q000494:pharmacology; D011963:Receptors, GABA-A / Q000032:analysis* / Q000276:immunology; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000276:immunology / Q000494:pharmacology
D000612:4-Aminobutyrate Transaminase; D000109:Acetylcholine; D000445:Aldehyde Oxidoreductases; D000906:Antibodies; D001640:Bicuculline; D001896:Boron Compounds; D002795:Choline O-Acetyltransferase; D005456:Fluorescent Dyes; D018755:GABA Agonists; D018756:GABA Antagonists; D009118:Muscimol; D010852:Picrotoxin; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; C046738:gabase