title: Expression analysis in plant and cell suspensions of CrCKR1, a cDNA encoding a histidine kinase receptor homologue in Catharanthus roseus (L.) G. Don.
abstract: A full length cDNA (CrCKR1) encoding a hybrid histidine kinase was isolated from a Catharanthus roseus cDNA library. The kinase belongs to the subfamily of cytokinin receptors represented by CRE1/AHK4/WOL in Arabidopsis thaliana. In cell suspensions, the expression of CrCKR1 is not affected by various stress and hormonal treatments but is stimulated in cells continuously exposed to cytokinin. In plants, CrCKR1 is strongly expressed only in the petals of mature flowers. These data suggest that CrCKR1 could take part in the mechanisms leading to the production of secondary metabolites in C. roseus.
authors.affiliation: EA 2106, Biomolécules et Biotechnologies Végétales, Université de Tours, 31 Avenue Monge, F-37200 Tours, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papon
authors.forename: Marc
authors.initials: M
authors.lastname: Clastre
authors.forename: Francoise
authors.initials: F
authors.lastname: Andreu
authors.forename: Pascal
authors.initials: P
authors.lastname: Gantet
authors.forename: Marc
authors.initials: M
authors.lastname: Rideau
authors.forename: Joël
authors.initials: J
authors.lastname: Crèche
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D030008:Catharanthus / Q000201:enzymology / Q000235:genetics*; D002478:Cells, Cultured; D003583:Cytokinins / Q000494:pharmacology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D000071677:Histidine Kinase; D008969:Molecular Sequence Data; D010802:Phylogeny; D018547:Plant Stems / Q000201:enzymology / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D029681:Arabidopsis Proteins; D003583:Cytokinins; D018076:DNA, Complementary; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011494:Protein Kinases; D000071677:Histidine Kinase; C421783:WOL protein, Arabidopsis