title: Dietary protein modifies hepatic gene expression associated with oxidative stress responsiveness in growing pigs.
abstract: Understanding the basis for differences in nutrient requirements and for nutrient effects on health and performance requires an appreciation of the links between nutrition and gene expression. We developed and applied molecular probes to characterize diet-associated postabsorptive hepatic gene expression in growing pigs chronically fed protein-restricted diets based on either casein (CAS) or soy protein isolate (SPI). Eighty-eight expressed sequence tags (ESTs) were identified on the basis of diet-related changes in expression, by using an mRNA differential display method. Expression profiling based on transcription analysis by real-time reverse transcriptase-polymerase chain reaction showed that the SPI diet significantly changed the pattern of gene expression as compared with the CAS diet and allowed identification of coregulated genes. The expression of six genes involved in the metabolism of stress response (glutathione S-transferase, peptide methionine sulfoxide reductase, apolipoprotein A-I, organic anion transport polypeptide 2, calnexin, heat shock transcription factor 1) exhibited significant changes in the transcription level and indicated an increased oxidative stress response in pigs fed the SPI diet. Hierarchical clustering of gene expression data of all 33 ESTs analyzed across 14 pigs fed the two different diets resulted in clustering of genes related to the oxidative stress response with genes related to the regulation of gene expression and neuronal signaling.
authors.affiliation: Research Units Molecular Biology, Research Institute for the Biology of Farm Animals, D-18196 Dummerstorf; Germany. schwerin@fbn-dummerstorf.de
authors.forename: Manfred
authors.initials: M
authors.lastname: Schwerin
authors.forename: Ute
authors.initials: U
authors.lastname: Dorroch
authors.forename: Manfred
authors.initials: M
authors.lastname: Beyer
authors.forename: Hermann
authors.initials: H
authors.lastname: Swalve
authors.forename: Cornelia C
authors.initials: CC
authors.lastname: Metges
authors.forename: Peter
authors.initials: P
authors.lastname: Junghans
mesh_terms: D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000494:pharmacology; D004044:Dietary Proteins / Q000494:pharmacology*; D020869:Gene Expression Profiling; D014644:Genetic Variation; D008099:Liver / Q000294:innervation / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D018384:Oxidative Stress; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D015398:Signal Transduction; D030262:Soybean Proteins / Q000494:pharmacology; D013552:Swine / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D002364:Caseins; D004044:Dietary Proteins; D012333:RNA, Messenger; D030262:Soybean Proteins