Hydrogen peroxide induces apoptosis of osteocytes: involvement of calcium ion and caspase activity.
We hypothesized that reactive oxygen species play an important role in avascular/ischemic osteonecrosis. When isolated chick osteocytes were cultured with hydrogen peroxide, annexin V binding, which is the earliest marker of apoptosis, increased in a dose-dependent fashion. Hydrogen peroxide also induced the activation of caspase-3 and increase in cytosolic Ca2+. Treatment with BAPTA/AM (cheletor of cytosolic Ca2+) and Ac-DEVD-cho (caspase inhibitor) attenuated hydrogen peroxide-induced apoptosis. These data demonstrated the signal transduction pathways that participate in this hydrogen peroxide-induced cell damage.
Kikuyama
A
A
Department of Orthopaedic Surgery, Kinki University School of Medicine, Ohno-higashi 377-2, Osaka-sayama, Osaka 589-8511, Japan.
Fukuda
K
K
Mori
S
S
Okada
M
M
Yamaguchi
H
H
Hamanishi
C
C
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000502:physiology*; D053148:Caspase 3; D061945:Caspase Inhibitors; D020169:Caspases / Q000096:biosynthesis*; D002478:Cells, Cultured; D002642:Chick Embryo; D003600:Cytosol / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology; D010011:Osteocytes / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D015398:Signal Transduction
D061945:Caspase Inhibitors; D009842:Oligopeptides; C099158:acetyl-aspartyl-glutamyl-valyl-aspartal; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D004533:Egtazic Acid; D006861:Hydrogen Peroxide; D053148:Caspase 3; D020169:Caspases; D002118:Calcium