title: MCP-1 is highly expressed in peritoneum following midline laparotomy with peritoneal abrasion in a murine model.
abstract: BACKGROUND
  Monocyte chemoattractant protein-1 (MCP-1), a CC chemokine, is a potent attractant of monocytes both in vitro and in vivo. However, its role in the repair of peritoneal injury is not well established. This study characterizes MCP-1 expression in surgical wounds following peritoneal abrasion in a murine model.
  
  
  METHODS
  Twenty-five C57 BL6 female mice underwent a 2-cm midline laparotomy with mechanical abrasion of the right peritoneal wall. The mice were sacrificed at various times ranging from 0 to 7 days. Hemotoxylin and eosin stained sections and tissue extracts were made using peritoneal samples from abraded and unabraded areas in each mouse. An enzyme-linked immunosorbent assay was performed on the specimens to quantitate MCP-1 expression. Values were compared using a t-test.
  
  
  RESULTS
  At baseline, there was minimal expression of MCP-1 (<5 pg/mg protein). Following surgery, MCP-1 levels at abraded sites were significantly higher than those at both baseline and unabraded sites at all times up to a week following surgery. Histologic evaluation revealed peritoneal thickening and leukocytic infiltration of only abraded surfaces.
  
  
  CONCLUSIONS
  MCP-1 is highly expressed in peritoneum following laparotomy with peritoneal abrasion. Elevations in MCP-1 levels are identified within 6 h of surgery and persist for up to 1 week. The histologic differences between abraded and unabraded areas may be attributable to differences in MCP-1 expression. Further studies using recombinant MCP-1 and anti-MCP-1 antibody may elucidate this relationship.
authors.affiliation: Minimally Invasive Surgery Center and Department of Immunology, the Cleveland Clinic Foundation, Cleveland, OH 44195, USA. brodyf@ccf.org
authors.forename: J A
authors.initials: JA
authors.lastname: Brodsky
authors.forename: F J
authors.initials: FJ
authors.lastname: Brody
authors.forename: B
authors.initials: B
authors.lastname: Endlich
authors.forename: D A
authors.initials: DA
authors.lastname: Armstrong
authors.forename: J L
authors.initials: JL
authors.lastname: Ponsky
authors.forename: I A
authors.initials: IA
authors.lastname: Hamilton
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000378:metabolism / Q000502:physiology; D005260:Female; D007813:Laparotomy / Q000379:methods*; D007962:Leukocytes / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity / Q000473:pathology / Q000601:surgery*; D010537:Peritoneum / Q000378:metabolism* / Q000473:pathology / Q000601:surgery*; D015854:Up-Regulation / Q000502:physiology; D014945:Wound Healing / Q000502:physiology
chemical_list: D018932:Chemokine CCL2