Cloning, Sequencing and Expression of Human Copper, Zinc-superoxide Dismutase cDNA.
The cDNA encoding the human copper, zinc-superoxide dismutase(Cu, Zn-SOD) was amplified from the human liver by RT-PCR and sequenced. The cloned human Cu, Zn-SOD cDNA was ligated into expression vector pET-22b(+) under T7 promotor. After 3 h induction with 1 mmol/L IPTG, human Cu, Zn-SOD was highly expressed in E.coli BL21(DE3). The expression product was up to 30% of the total protein of the bacteria in soluble form, which had specific SOD activity.
Shi
Hui-Juan
HJ
Research Institute of Biochemistry, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. qsyuan@ecust.edu.cn
Fan
Li-Qiang
LQ
Wei
Dong-Zhi
DZ
Yuan
Qin-Sheng
QS
Sun
Jian-Xing
JX
Wu
Xiang-Fu
XF