The pepR gene of Lactobacillus sakei is positively regulated by anaerobiosis at the transcriptional level.
Lactobacillus sakei is a lactic acid bacterium belonging to the natural flora of meat products. It constitutes the main flora of vacuum-packed meat and is largely used in western Europe as a starter for the manufacturing of fermented sausages. This species is able to grow both under aerobiosis and anaerobiosis. In many technological processes involving it, oxygen is scarce. The aim of this study was to identify the major proteins affected by growth under anaerobiosis. Using two-dimensional electrophoresis, we showed that one spot was 10-fold overexpressed when cells were grown under anaerobiosis. By N-terminal sequencing it was identified as a peptidase (PepR), and the pepR gene was cloned. Northern analysis revealed that pepR was expressed as a single 1.27-kb transcript induced under anaerobiosis. A mutant was constructed by single crossover in the pepR gene, and its growth and survival were not affected by anaerobiosis.
Champomier-Vergès
Marie-Christine
MC
Unité Flore Lactique et Environnement Carné, INRA, Domaine de Vilvert, 78 350 Jouy en Josas, France. verges@jouy.inra.fr
Marceau
Anika
A
Méra
Thérèse
T
Zagorec
Monique
M
D000332:Aerobiosis; D000626:Aminopeptidases / Q000235:genetics* / Q000378:metabolism; D000693:Anaerobiosis; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D007778:Lactobacillus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D003470:Culture Media; D000626:Aminopeptidases; C427186:pepR protein, Listeria monocytogenes