title: Biological effects of G1 phase arrest compound, sesquicillin, in human breast cancer cell lines.
abstract: Sesquicillin, isolated from fungal fermentation broth, strongly induced G1 phase arrest in human breast cancer cells. During G1 phase arrest, the expression level of cyclin D1, cyclin A, and cyclin E was decreased, and the expression of CDK (cyclin-dependent-kinase) inhibitor, protein p21(Waf1/Cip1), was increased in a time-dependent manner in a breast cancer cell MCF-7. Interestingly, the G1 phase arrest induced by sesquicillin also occurred independently of the tumor suppressor protein, p53. Sesquicillin inhibits the proliferation of MCF-7 via G1 phase arrest in association with the induction of CDK inhibitor protein, p21(Waf1/Cip1), and the reduction of G1 phase related-cyclin proteins.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yoosung, Taejon, Republic of Korea.
authors.forename: Ha Won
authors.initials: HW
authors.lastname: Jeong
authors.forename: Ho Jae
authors.initials: HJ
authors.lastname: Lee
authors.forename: Yung Hee
authors.initials: YH
authors.lastname: Kho
authors.forename: Kwang Hee
authors.initials: KH
authors.lastname: Son
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Han
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Lim
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Lee
authors.forename: Dong Cho
authors.initials: DC
authors.lastname: Han
authors.forename: Ji Hong
authors.initials: JH
authors.lastname: Ha
authors.forename: Byoung Mog
authors.initials: BM
authors.lastname: Kwon
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology*; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005658:Fungi / Q000737:chemistry; D016193:G1 Phase / Q000187:drug effects*; D006801:Humans; D009281:Naphthalenes / Q000302:isolation & purification / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000494:pharmacology
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D009281:Naphthalenes; D016159:Tumor Suppressor Protein p53; C113328:sesquicillin