title: Effect of deficiency of vitamins C and/or E on lipoprotein metabolism in osteogenic disorder Shionogi rat, a strain unable to synthesize ascorbic acid.
abstract: The effects of vitamin C and/or E deficiency on lipoprotein metabolism were investigated in the inherently scorbutic Osteogenic Disorder Shionogi (ODS) rat. In the vitamin C-deficient (C-def) group, marked increases in plasma VLDL and LDL cholesterol were observed (by comparison with the vitamins C- and E-sufficient control group). In rats kept deficient in both vitamin C and vitamin E (C,E-def), LDL cholesterol was significantly higher than in the C-def group even though the levels of VLDL and HDL cholesterol were similar between the two groups. TBARS values for the LDL fraction in the C-def group were of the same magnitude as in the E-def group, and these values were significantly higher than those obtained for the control group. In the C,E-def group, the values were even higher than in the E-def and C-def groups. The nondenatured PAGE of the LDL fraction indicated the appearance of HDLc in the C-def and C,E-def groups. The SDS-PAGE of the LDL fraction showed increased apo B-48 in the C-def and C,E-def groups and increased apo E in the C,E-def group. Decreased plasma LCAT activity in the E-def, C-def, and C,E-def groups indicated an alteration in HDL metabolism as a result of oxidation. These results suggest that lipid peroxidation and some distinct features of lipoprotein metabolism resulting from vitamin C deficiency become more significant when vitamin E is also deficient along with vitamin C deficiency.
authors.affiliation: Gene Research Center, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. kammy@biochem.tohoku.ac.jp
authors.forename: Shin
authors.initials: S
authors.lastname: Kamiyama
authors.forename: Zakir H
authors.initials: ZH
authors.lastname: Howlader
authors.forename: Michiko
authors.initials: M
authors.lastname: Ito
authors.forename: Michio
authors.initials: M
authors.lastname: Komai
authors.forename: Yuji
authors.initials: Y
authors.lastname: Furukawa
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage*; D001206:Ascorbic Acid Deficiency / Q000378:metabolism*; D001835:Body Weight; D008078:Cholesterol, LDL / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains; D011922:Rats, Mutant Strains; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage*; D014811:Vitamin E Deficiency / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D008055:Lipids; D008074:Lipoproteins; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D001205:Ascorbic Acid