Novel rhenium(I) polypyridine biotin complexes that show luminescence enhancement and lifetime elongation upon binding to avidin.
While most biotin-fluorophore conjugates suffer from significant emission quenching upon binding to avidin due to resonance energy-transfer, three novel rhenium(I) polypyridine biotin complexes have been designed in view of their characteristic photophysical properties, in particular their large Stokes shifts. In contrast to most biotin-fluorophore conjugates, the (3)MLCT emission intensities and lifetimes of these rhenium(I) complexes are increased upon binding to avidin, rendering them luminescent probes for avidin and biotinylated species.
Lo
Kenneth Kam-Wing
KK
Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, P. R. China. bhkenlo@cityu.edu.hk
Hui
Wai-Ki
WK
Ng
Dominic Chun-Ming
DC
D001360:Avidin / Q000378:metabolism*; D001710:Biotin / Q000737:chemistry* / Q000378:metabolism; D008163:Luminescent Measurements; D011485:Protein Binding; D011725:Pyridines / Q000737:chemistry* / Q000378:metabolism; D012211:Rhenium / Q000737:chemistry* / Q000378:metabolism
D011725:Pyridines; D001360:Avidin; D001710:Biotin; D012211:Rhenium; C023666:pyridine