title: Phosphomonoester concentrations differ between chronic lymphocytic leukemia cells and normal human lymphocytes.
abstract: Levels of phospholipid-related metabolites of chronic lymphocytic leukemia lymphocytes (CLL) and normal human lymphocytes were quantified using phosphorus magnetic resonance spectroscopy. The CLL cells versus normal lymphocytes showed significant increases of phosphoethanolamine(Etn-P) (8.11+/-2.10 mean+/-S.E., micromol/g wet weight, n=12 versus 3.63+/-1.10, n=3, P<or=0.002), phosphocholine (2.10+/-0.37, n=12 versus 0.36+/-0.09, n=3, P<or=0.01), glycerophosphoethanolamine (0.26+/-0.03, n=10 versus 0.11+/-0.05, n=3, P<or=0.004), and glycerophosphocholine (0.33+/-0.03, n=10 versus 0.17+/-0.05, n=3, P<or=0.003). Further, the phospholipid precursor ethanolamine (Eth) was studied in blood and was found significantly lowered in CLL patients (4.6+/-1.6 microM, n=25) compared to normal volunteers (7.7+/-2.5, n=12, P<or=0.001). Increased intermediates with depletion of precursors suggest the presence of sustained phospholipid metabolism activation in CLL.
authors.affiliation: NMR and Medical Spectroscopy, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA.
authors.forename: Suzanne E
authors.initials: SE
authors.lastname: Franks
authors.forename: Mitchell R
authors.initials: MR
authors.lastname: Smith
authors.forename: Fernando
authors.initials: F
authors.lastname: Arias-Mendoza
authors.forename: Calvin
authors.initials: C
authors.lastname: Shaller
authors.forename: Kristen
authors.initials: K
authors.lastname: Padavic-Shaller
authors.forename: Francis
authors.initials: F
authors.lastname: Kappler
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: William G
authors.initials: WG
authors.lastname: Negendank
authors.forename: Truman R
authors.initials: TR
authors.lastname: Brown
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004983:Ethanolamines / Q000378:metabolism; D005260:Female; D005997:Glycerylphosphorylcholine / Q000378:metabolism; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019906:Nuclear Magnetic Resonance, Biomolecular; D009711:Nucleotides / Q000378:metabolism; D010755:Organophosphates / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D010759:Phosphorus Isotopes; D010767:Phosphorylcholine / Q000378:metabolism
chemical_list: D004983:Ethanolamines; D009711:Nucleotides; D010755:Organophosphates; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010759:Phosphorus Isotopes; D010767:Phosphorylcholine; C002449:glycerophosphoethanolamine; D005997:Glycerylphosphorylcholine; C005448:phosphorylethanolamine