Synthesis of 99mTc-ciprofloxacin by different methods and its biodistribution.
We describe here the synthesis of 99mTc-ciprofloxacin by four different methods and its biodistribution. All of the methods gave high radiochemical yields of > or = 90% and high stability of > or = 90% at 6 h after preparation. However HPLC analysis, bacterial binding assay, and in vivo distribution for the four 99mTc-ciprofloxacins showed different results. Among these methods, the use of formamidine sulfinic acid with microwave heating (Method A) was fast and easy, and gave more desirable biological properties than the other methods.
Oh
Seung Jun
SJ
Department of Nuclear Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Songpa-gu, Seoul, South Korea. sjoh@www.amc.seoul.kr
Ryu
Jin-Sook
JS
Shin
Joong Woo
JW
Yoon
Eun Jin
EJ
Ha
Hyun-Joon
HJ
Cheon
Joon Hong
JH
Lee
Hee Kyung
HK
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D008297:Male; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D011854:Radiochemistry; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism; D014018:Tissue Distribution
D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D002939:Ciprofloxacin