title: Hemolysis of human red blood cells by combination of riboflavin and aminophylline.
abstract: The effect of aminophylline on human red blood cells (RBC) has been studied. Under in vitro condition, aminophylline alone does not hemolyse RBC. However, in the presence of riboflavin and visible light, aminophylline causes hemolysis of RBC. This hemolysis depends on the concentration of both riboflavin and aminophylline. Using different free radical scavengers we show that RBC hemolysis is caused by reactive oxygen species. Studies using bovine serum albumin show that riboflavin-aminophylline combination can also cause protein degradation in vitro.
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, India. iyad74@lycos.com
authors.forename: Lyad
authors.initials: L
authors.lastname: Ali
authors.forename: Imrana
authors.initials: I
authors.lastname: Naseem
mesh_terms: D000628:Aminophylline / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008027:Light; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D011188:Potassium / Q000097:blood; D012256:Riboflavin / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D016166:Free Radical Scavengers; D010726:Phosphodiesterase Inhibitors; D012710:Serum Albumin, Bovine; D000628:Aminophylline; D003300:Copper; D011188:Potassium; D012256:Riboflavin