Mutational analysis of the TolA C-terminal domain of Escherichia coli and genetic evidence for an interaction between TolA and TolB.
The Tol proteins are involved in the outer membrane stability of gram-negative bacteria. The C-terminal domain of TolA was mutagenized to identify residues important for its functions. The isolation of suppressor mutants of tolA mutations in the tolB gene confirmed an interaction between TolAIII and the N-terminal domain of TolB.
Dubuisson
Jean François
JF
Unité de Microbiologie et Génétique, UMR5122 (CNRS-INSA-Université Lyon1), F-69622 Villeurbanne Cedex, France.
Vianney
Anne
A
Lazzaroni
Jean Claude
JC
D000595:Amino Acid Sequence; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000502:physiology; D033903:Periplasmic Proteins; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary
D029968:Escherichia coli Proteins; D033903:Periplasmic Proteins; C062093:tolA protein, E coli; C062094:tolB protein, E coli