title: In vitro and in vivo study of dye diffusion into the human skin and hair follicles.
abstract: We present experimental results on the in vitro and in vivo study of dye diffusion into human skin and hair follicles. We have studied some commercially available dyes for potential using in the laser selective thermolysis. The degree and the depth of hair follicle dyeing inside the skin were determined. For hairs in different stages the sebaceous gland was stated as a reservoir for a dye administration. It was found that the penetration depth of dyes is about 1.2 mm from the skin surface. We have developed the biocompatible Indocyanine Green lotions and the method for in vivo dyeing and dye in depth monitoring. Shift on 16-21 nm of absorption peak of Indocyanine Green to the longer wavelengths due to Indocyanine Green binding with cell proteins in the human skin was found.
authors.affiliation: Saratov State University, Optics Department, Saratov, 410026 Russia. eagenina@optics.sgu.ru
authors.forename: Elina A
authors.initials: EA
authors.lastname: Genina
authors.forename: Alexey N
authors.initials: AN
authors.lastname: Bashkatov
authors.forename: Yury P
authors.initials: YP
authors.lastname: Sinichkin
authors.forename: Vyacheslav I
authors.initials: VI
authors.lastname: Kochubey
authors.forename: Nina A
authors.initials: NA
authors.lastname: Lakodina
authors.forename: Gregory B
authors.initials: GB
authors.lastname: Altshuler
authors.forename: Valery V
authors.initials: VV
authors.lastname: Tuchin
mesh_terms: D004396:Coloring Agents / Q000493:pharmacokinetics*; D004058:Diffusion; D018859:Hair Follicle / Q000033:anatomy & histology*; D006204:Hair Removal / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D053685:Laser Therapy / Q000379:methods; D012867:Skin / Q000033:anatomy & histology*; D012871:Skin Diseases / Q000601:surgery; D013053:Spectrophotometry
chemical_list: D004396:Coloring Agents