title: Multiple Mycobacterium tuberculosis strains in early cultures from patients in a high-incidence community setting.
abstract: In an ongoing molecular epidemiology study, human immunodeficiency virus-negative patients with first-time pulmonary tuberculosis from a high-incidence community were enrolled. Mycobacterium tuberculosis strains were identified by restriction fragment length polymorphism analysis with two fingerprinting probes. Of 131 patients, 3 (2.3%) were shown to have a mixture of strains in one or two of their serial cultures. This study further investigated these cases with disease caused by multiple M. tuberculosis strains in the context of the molecular epidemiology of the study setting.
authors.affiliation: MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa.
authors.forename: Madalene
authors.initials: M
authors.lastname: Richardson
authors.forename: Nora M
authors.initials: NM
authors.lastname: Carroll
authors.forename: Erica
authors.initials: E
authors.lastname: Engelke
authors.forename: Gian D
authors.initials: GD
authors.lastname: Van Der Spuy
authors.forename: Faeeza
authors.initials: F
authors.lastname: Salker
authors.forename: Zahn
authors.initials: Z
authors.lastname: Munch
authors.forename: Robert P
authors.initials: RP
authors.lastname: Gie
authors.forename: Robin M
authors.initials: RM
authors.lastname: Warren
authors.forename: Nulda
authors.initials: N
authors.lastname: Beyers
authors.forename: Paul D
authors.initials: PD
authors.lastname: Van Helden
mesh_terms: D000328:Adult; D003470:Culture Media; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D014644:Genetic Variation; D006801:Humans; D015994:Incidence; D008297:Male; D017720:Molecular Epidemiology; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D003470:Culture Media; D004251:DNA Transposable Elements; D004269:DNA, Bacterial