title: [A new oxidase method for analyzing L-lactate].
abstract: A new oxidase-coupled colorimetric method for analysis of L-lactate in biological fluids has been developed without use of peroxidase. The method is based on lactate oxidase-catalysed transformation of lactate to pyruvate which is determined photometrically in the next dye-producing reaction of 3-methyl-2-benzothiazolinone hydrazone (MBTH) in the presence of ferric ions. Sensitivity of the method is estimated as 0.1 micromole of analyte in 4-ml of reaction mixture. Linearity is observed in the range 0.1-1.0 micromole of L-lactate in sample (r = 0.99943; p < 0.0001). The developed method has been adapted for assay of L-lactic acid in kefirs and yogurts.
authors.affiliation: Institute of Cell Biology, NAS of Ukraine, Lviv.
authors.forename: H M
authors.initials: HM
authors.lastname: Pavlishko
authors.forename: M M
authors.initials: MM
authors.lastname: Maĭdan
authors.forename: A A
authors.initials: AA
authors.lastname: Sybirnyĭ
authors.forename: M V
authors.initials: MV
authors.lastname: Honchar
mesh_terms: D002384:Catalysis; D003124:Colorimetry; D019344:Lactic Acid / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D019344:Lactic Acid; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase