Fluorescence Analysis of the Fibrinolytic Principle (FP) from Agkistrodon acutus Venom.
The conformation and the properties of the fibrinolytic principle (FP) from Agkistrodon acutus venom were studied by chemical modification and fluorescence spectroscopy. Results showed that there are more than one tryptophan (Trp) residue in the FP molecule and they are located in the more hydrophobic core, could be quenched by acrylamide (Acr), a polarized quencher without electric charge. The collisional quenching constants of FP at different concentrations of Acr were calculated in terms of Stern-Volmer equation, and the fraction of the Trp quenched was obtained by the modified Stern-Volmer equation as 83%.
Wang
Shou-Ye
SY
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China. lab305@mail.ach.ustc.edu.cn
Yu
Hua-Ming
HM
Chen
Zhen-Long
ZL
Zhang
Zu-De
ZD
Wu
Shuang-Ding
SD
Liu
Qing-Liang
QL