DNA fingerprinting of Pasteurella multocida recovered from avian sources.
Repetitive sequence-based PCR (rep-PCR) and amplified fragment length polymorphism (AFLP) were used to characterize a sample of 43 field isolates and 4 attenuated vaccine strains of Pasteurella multocida recovered from multiple avian species. Both rep-PCR and AFLP assays were rapid and reproducible, with high indices of discrimination. Concordance analyses of rep-PCR and AFLP with somatic serotyping indicate that, in general, somatic serotyping is a poor indicator of genetic relatedness among isolates of P. multocida. In addition, the data provide evidence of host specificity of P. multocida clones. Overall, the results of our study indicate that the rep-PCR and AFLP techniques enable rapid fingerprinting of P. multocida isolates from multiple avian species and enhance the investigation of fowl cholera outbreaks.
Amonsin
Alongkorn
A
Biomedical Genomics Center and Department of Veterinary Pathobiology, University of Minnesota, St. Paul 55108, USA.
Wellehan
James F X
JF
Li
Ling-Ling
LL
Laber
Judy
J
Kapur
Vivek
V
D000818:Animals; D015373:Bacterial Typing Techniques; D001715:Bird Diseases / Q000453:epidemiology* / Q000382:microbiology; D001717:Birds; D002645:Chickens; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D010326:Pasteurella Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D011201:Poultry Diseases / Q000453:epidemiology* / Q000382:microbiology; D015203:Reproducibility of Results; D012984:Software; D014422:Turkeys
D004269:DNA, Bacterial