title: Conjugative transfer of the erm(A) gene from erythromycin-resistant Streptococcus pyogenes to macrolide-susceptible S. pyogenes, Enterococcus faecalis and Listeria innocua.
abstract: In mating experiments, the erythromycin resistance methylase gene erm(A) was successfully transferred from erm(A)-positive clinical isolates of Streptococcus pyogenes to macrolide-susceptible recipients of S. pyogenes, Enterococcus faecalis and Listeria innocua. Compared with the SmaI macrorestriction pattern of the S. pyogenes recipient, the patterns of S. pyogenes transconjugants shared the lack of a fragment and the appearance of a new, larger fragment. This is the first experimental evidence that the erm(A) gene can be transferred from erythromycin-resistant S. pyogenes to macrolide-susceptible S. pyogenes as well as to other Gram-positive recipients.
authors.affiliation: Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131 Ancona, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Giovanetti
authors.forename: G
authors.initials: G
authors.lastname: Magi
authors.forename: A
authors.initials: A
authors.lastname: Brenciani
authors.forename: C
authors.initials: C
authors.lastname: Spinaci
authors.forename: R
authors.initials: R
authors.lastname: Lupidi
authors.forename: B
authors.initials: B
authors.lastname: Facinelli
authors.forename: P E
authors.initials: PE
authors.lastname: Varaldo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D003227:Conjugation, Genetic / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004917:Erythromycin / Q000494:pharmacology*; D006801:Humans; D008087:Listeria / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008780:Methyltransferases; D013297:Streptococcus pyogenes / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004917:Erythromycin; D008780:Methyltransferases; C431709:ErmA protein, Bacteria