title: Cryopreservation of protonemata of Ditrichum cornubicum (paton) comparing the effectiveness of four cryoprotectant pretreatments.
abstract: If an ex situ programme for the conservation of rare and threatened bryophytes is to succeed, a method of long-term, basal storage is required. The aim of this study was to determine the effectiveness of sucrose, ABA and sucrose/ABA combined, as cryoprotectant pretreatments, for the long-term storage of protonemata of the endangered Cornish path moss, Ditrichum cornubicum. This work is a prelude to the in vitro conservation of rare and endangered UK bryophytes. The addition of sucrose and/or ABA in the pre-culture medium, reduced protonemal growth rate. However, pretreatment afforded a high level of protection against tissue damage during dehydration and freezing, with 100 % regeneration of pretreated plants after thawing, compared to 53 % regeneration of controls. Sucrose combined with ABA in the pre-culture medium gave the most effective cryoprotection, with protonemata having fewer necrotic tissues and rapid recovery of growth.
authors.affiliation: Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AB, UK.
authors.forename: J
authors.initials: J
authors.lastname: Burch
authors.forename: T
authors.initials: T
authors.lastname: Wilkinson
mesh_terms: D019068:Bryopsida / Q000254:growth & development; D015925:Cryopreservation; D003451:Cryoprotective Agents; D013395:Sucrose
chemical_list: D003451:Cryoprotective Agents; D013395:Sucrose