title: Application of a Designed Synthetic Ligand for the Separation and Purification of Alkaline Phosphatase.
abstract: An artificial affinity ligand comprising a p-aminobenzyl phosphoric acid as inhibitor was designed and coupled to an agarose support by chlorotriazine ring to separate and purify alkaline phosphatase from calf intestine. The optimal density of the ligand is 6 &mgr;mol/g adsorbent. After elution with sodium chloride and inorganic phosphoric acid a 300-fold purified enzymatic preparation was obtained in one step with a yield of 90%.
authors.affiliation: Institute of Biochemical Engineering, School of Chemical Engineering, Dalian University of Technology, Dalian 116012, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Yao
authors.forename: Ming-Li
authors.initials: ML
authors.lastname: Peng
authors.forename: Kun-Yu
authors.initials: KY
authors.lastname: Gao
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Su