title: Coagulation, fibrinolysis, and cell activation in patients and shed mediastinal blood during coronary artery bypass grafting with a new heparin-coated surface.
abstract: OBJECTIVE
  Heparin coating of the cardiopulmonary bypass circuit is shown to improve the biocompatibility of the surface. We have studied a new heparin surface, the Corline Heparin Surface, applied to a complete set of an extracorporeal device used during coronary artery bypass grafting in terms of activation of inflammation, coagulation, and fibrinolysis in patients and in shed mediastinal blood.
  
  
  METHODS
  Sixty patients scheduled for coronary artery bypass grafting were randomized to one of 3 groups with heparin-coated devices receiving either a standard, high, or low dose of systemic heparin or to an uncoated but otherwise identical circuit receiving a standard dose of systemic heparin. Samples were drawn before, during, and after the operation from the pericardial cavity and in shed mediastinal blood. No autotransfusion of shed mediastinal blood was performed.
  
  
  RESULTS
  The Corline Heparin Surface significantly reduced the activation of coagulation, fibrinolysis, platelets, and inflammation compared with that seen with the uncoated surface in combination with a standard dose of systemic heparin during cardiac surgery with cardiopulmonary bypass. Both a decrease and an increase of systemic heparin in combination with the coated heparin surface resulted in higher activation of these processes. A significantly higher expression of all studied parameters was found in the shed mediastinal blood compared with in systemic blood at the same time.
  
  
  CONCLUSIONS
  The Corline Heparin Surface used in cardiopulmonary bypass proved to be more biocompatible than an uncoated surface when using a standard systemic heparin dose. The low dose of systemic heparin might not be sufficient to maintain the antithrombotic activity, and the high dose resulted in direct cell activation rather than a further anti-inflammatory and anticoagulatory effect.
authors.affiliation: Department of Medical Sciences, Clinical Chemistry, Laboratory for Coagulation Research, University Hospital, SE-751 85 Uppsala, Sweden.
authors.forename: Matilda
authors.initials: M
authors.lastname: Johnell
authors.forename: Graciela
authors.initials: G
authors.lastname: Elgue
authors.forename: Rolf
authors.initials: R
authors.lastname: Larsson
authors.forename: Anders
authors.initials: A
authors.lastname: Larsson
authors.forename: Stefan
authors.initials: S
authors.lastname: Thelin
authors.forename: Agneta
authors.initials: A
authors.lastname: Siegbahn
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D000925:Anticoagulants / Q000008:administration & dosage*; D001779:Blood Coagulation Factors / Q000032:analysis; D016063:Blood Loss, Surgical / Q000517:prevention & control*; D020099:Coated Materials, Biocompatible; D001026:Coronary Artery Bypass; D016207:Cytokines / Q000097:blood; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005434:Flow Cytometry; D006493:Heparin / Q000008:administration & dosage*; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D018709:Statistics, Nonparametric; D016896:Treatment Outcome
chemical_list: D000925:Anticoagulants; D001779:Blood Coagulation Factors; D020099:Coated Materials, Biocompatible; D016207:Cytokines; D005338:Fibrin Fibrinogen Degradation Products; D006493:Heparin