title: Multilaboratory comparison of anaerobe susceptibility results using 3 different agar media.
abstract: A 5-laboratory study was performed that used the National Committee for Clinical Laboratory Standards (NCCLS) reference agar dilution method with 3 media formulations to determine whether the use of different media would affect minimum inhibitory concentration (MIC) results. Wilkins-Chalgren, Brucella-based blood agar (BRU), and Wilkins-Chalgren agar plus blood (WCB) and 6 antibiotics (clindamycin, cefoxitin, ceftizoxime, piperacillin, metronidazole, and trovafloxacin) were evaluated with 58 isolates. The MIC values were compared, and a significant correlation of >0.80 was demonstrated for all media and each antibiotic/organism group. The cumulative rate of errors for all antibiotics was 0.1%. These data indicate that a change in the NCCLS reference medium for testing of anaerobic bacteria susceptibility to either BRU or WCB will not affect the MIC results for the antibiotics and organisms evaluated.
authors.affiliation: Anaerobe Systems, Morgan Hill, CA, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Roe
authors.forename: S M
authors.initials: SM
authors.lastname: Finegold
authors.forename: D M
authors.initials: DM
authors.lastname: Citron
authors.forename: E J C
authors.initials: EJ
authors.lastname: Goldstein
authors.forename: H M
authors.initials: HM
authors.lastname: Wexler
authors.forename: J E
authors.initials: JE
authors.lastname: Rosenblatt
authors.forename: M E
authors.initials: ME
authors.lastname: Cox
authors.forename: S G
authors.initials: SG
authors.lastname: Jenkins
authors.forename: D W
authors.initials: DW
authors.lastname: Hecht
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000302:isolation & purification; D001769:Blood; D003470:Culture Media; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010837:Vitamin K 1 / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D006427:Hemin; D010837:Vitamin K 1