title: Effects of repeated subculturing and prolonged storage at room temperature of enterohemorrhagic Escherichia coli O157:H7 on pulsed-field gel electrophoresis profiles.
abstract: Three clinical strains of enterohemorrhagic Escherichia coli O157:H7 which were subcultured repeatedly or stored at room temperature over a 25-week period showed appreciable variations in their pulsed-field gel electrophoresis fragment patterns. The variations could be explained by a couple of spontaneous genetic events at most and thus did not invalidate the genetic lineage of the strains.
authors.affiliation: Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Iguchi
authors.forename: Ro
authors.initials: R
authors.lastname: Osawa
authors.forename: Junichi
authors.initials: J
authors.lastname: Kawano
authors.forename: Akira
authors.initials: A
authors.lastname: Shimizu
authors.forename: Jun
authors.initials: J
authors.lastname: Terajima
authors.forename: Haruo
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D014644:Genetic Variation; D006801:Humans; D012692:Serial Passage; D013696:Temperature
chemical_list: D004269:DNA, Bacterial