title: A zinc metalloprotease inhibitor, Inh, from the insect pathogen Photorhabdus luminescens.
abstract: The entomopathogen Photorhabdus luminescens secretes many proteins during the late stages of insect larvae infection and during in vitro laboratory culture. The authors have previously characterized and purified a 55 kDa zinc metalloprotease, PrtA, from culture supernatants of P. luminescens. PrtA is secreted via a classical type I secretory pathway and is encoded within the operon prtA-inh-prtBCD. The 405 bp inh gene encodes a 14.8 kDa pre-protein that is translocated to the periplasm by the classical signal-peptide-dependent sec pathway, yielding the mature 11.9 kDa inhibitor Inh. Inh is a specific inhibitor of the protease PrtA. This study describes the purification of Inh and the initial characterization of its in vitro protease inhibition properties.
authors.affiliation: Institut de Génétique et Microbiologie, Laboratoire de Pathogenèse Comparée, CNRS UMR 8621, Bâtiment 360, Université Paris XI, 91405 Orsay Cedex, France1.
authors.forename: Michèle
authors.initials: M
authors.lastname: Valens
authors.affiliation: Institut de Génétique et Microbiologie, Laboratoire de Pathogenèse Comparée, CNRS UMR 8621, Bâtiment 360, Université Paris XI, 91405 Orsay Cedex, France1.
authors.forename: Anne-Cécile
authors.initials: AC
authors.lastname: Broutelle
authors.affiliation: Institut de Génétique et Microbiologie, Laboratoire de Pathogenèse Comparée, CNRS UMR 8621, Bâtiment 360, Université Paris XI, 91405 Orsay Cedex, France1.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Lefebvre
authors.affiliation: Institut de Génétique et Microbiologie, Laboratoire de Pathogenèse Comparée, CNRS UMR 8621, Bâtiment 360, Université Paris XI, 91405 Orsay Cedex, France1.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Blight
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors; D008969:Molecular Sequence Data; D005810:Multigene Family; D020637:Photorhabdus / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D015032:Zinc / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C093610:Inh protein, bacteria; D008666:Metalloendopeptidases; D015032:Zinc