title: Excitotoxic injury induces production of monocyte chemoattractant protein-1 in rat cortico-striatal slice cultures.
abstract: The effect of excitotoxic injury on the production of monocyte chemoattractant protein-1 (MCP-1) was examined in rat cortico-striatal slice cultures. Treatment with 50 microM N-methyl-D-aspartate (NMDA) for 4 h, which caused severe damage in neurons, induced the production of MCP-1 in astrocytes. Production levels were markedly elevated immediately after the treatment, peaked at 4-8 h, and had decreased to nearly the basal level by 72 h. Since the treatment promoted the release of MCP-1 in the slice cultures, but not in the enriched astrocyte cultures, it is unlikely that NMDA directly acted on the astrocytes. These results suggest that information on neuronal injury induced by NMDA is transmitted to astrocytes to induce the production of MCP-1. Organotypic slice cultures are useful for investigating the inflammatory responses of astrocytes in the process of neuronal injury.
authors.affiliation: Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, 606-8501, Kyoto, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Katayama
authors.forename: Masabumi
authors.initials: M
authors.lastname: Minami
authors.forename: Mika
authors.initials: M
authors.lastname: Nakamura
authors.forename: Misato
authors.initials: M
authors.lastname: Ito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Katsuki
authors.forename: Akinori
authors.initials: A
authors.lastname: Akaike
authors.forename: Masamichi
authors.initials: M
authors.lastname: Satoh
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D066298:In Vitro Techniques; D009474:Neurons / Q000473:pathology; D009498:Neurotoxins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D018932:Chemokine CCL2; D009498:Neurotoxins