Effects of repeated subculturing and prolonged storage at room temperature of enterohemorrhagic Escherichia coli O157:H7 on pulsed-field gel electrophoresis profiles.
Three clinical strains of enterohemorrhagic Escherichia coli O157:H7 which were subcultured repeatedly or stored at room temperature over a 25-week period showed appreciable variations in their pulsed-field gel electrophoresis fragment patterns. The variations could be explained by a couple of spontaneous genetic events at most and thus did not invalidate the genetic lineage of the strains.
Iguchi
Atsushi
A
Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Japan.
Osawa
Ro
R
Kawano
Junichi
J
Shimizu
Akira
A
Terajima
Jun
J
Watanabe
Haruo
H
D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D014644:Genetic Variation; D006801:Humans; D012692:Serial Passage; D013696:Temperature
D004269:DNA, Bacterial