title: Two novel oligosaccharides formed by 1F-fructosyltransferase purified from roots of asparagus (Asparagus officinalis L.).
abstract: Two novel oligosaccharides, tetra-and penta-saccharides were synthesized by fructosyl transfer from 1-kestose to 4G-beta-D-galactopyranosylsucrose with a purified 1F-fructosyltransferase of asparagus roots and identified as 1F-beta-D-fructofuranosyl-4G-beta-D-galactopyranosylsucrose, O-beta-D-fructofuranosyl-(2-->1)-beta-D-fructofuranosyl-O-[beta-D-galactopyranosyl-(1-->4)]-alpha-D-glucopyranoside and 1F(1-beta-D-fructofuranosyl)2-4G-beta-D-galactopyranosylsucrose, [O-beta-D-fructofuranosyl-(2-->1)]2-beta-D-fructofuranosyl-O-[beta-D-galactopyranosyl-(1-->4)]-alpha-D-glucopyranoside, respectively. Both oligosaccharides were scarcely hydrolyzed by carbohydrase from rat small intestine. Human intestinal bacterial growth by 1F-beta-D-fructofuranosyl-4G-beta-D-galactopyranosylsucrose was compared with that by the tetrasaccharides, stachyose and nystose. Bifidobacteria utilized 1F-beta-D-fructofuranosyl-4G-beta-D-galactopyranosylsucrose to the same extent as stachyose or nystose. On the other hand, the unfavorable bacteria, Clostridium perfringens, Escherichia coli and Enterococcusfaecalis, that produce mutagenic substances did not use the synthetic oligosaccharide.
authors.affiliation: Department of Food Production Unit and Utility Development, Graduate School of Dairy Science Research, Rakuno Gakuen University, Ebetsu, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Yamamori
authors.forename: Shuichi
authors.initials: S
authors.lastname: Onodera
authors.forename: Masanori
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Norio
authors.initials: N
authors.lastname: Shiomi
mesh_terms: D000818:Animals; D027761:Asparagus Plant / Q000201:enzymology*; D001644:Bifidobacterium / Q000378:metabolism; D002240:Carbohydrate Sequence; D003016:Clostridium perfringens / Q000378:metabolism; D016972:Enterobacter cloacae / Q000378:metabolism; D013293:Enterococcus faecalis / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006602:Hexosyltransferases / Q000302:isolation & purification* / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007421:Intestine, Small / Q000201:enzymology; D007778:Lactobacillus / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology*; D051381:Rats; D001426:Bacterial Proteins
chemical_list: D006026:Glycoside Hydrolases; D006602:Hexosyltransferases; D009844:Oligosaccharides; C021451:inulosucrase; D001426:Bacterial Proteins; C019822:carbohydrase