A direct sandwich ELISA to detect antibodies against the C-terminal region of merozoite surface protein 1 could be a useful diagnostic method to identify Plasmodium vivax exposed persons.
We expressed a C-terminal 108-aa region of the Plasmodium vivax merozoite surface protein 1 (PvMSP1c) excluding the C-end transmembrane region in Escherichia coli in order to evaluate the antibody level to MSP in Korean malaria patients. We optimized a direct sandwich enzyme-linked immunosorbent assay (ELISA) method to simultaneously determine the total antibody levels, including IgG and IgM, to PvMSP1c. If the cut-off for seropositivity was determined as the mean+3SD of the antibody levels of the negative control group, the antibody levels were positive in 99.5% of the patient group (sensitivity 199/200). The antibody levels were negative in 99.4% of the negative control group (specificity 504/507). The positive reactions in the negative control group came from non-specific reactions, as confirmed by a competition assay. This direct sandwich ELISA for PvMSP1c antibody could prove to be a useful tool for the diagnosis of malaria patients and for blood screening in blood banks.
Lim
Kook Jin
KJ
Department of Biochemistry, College of Science, Protein Network Research Center, Yonsei University, Seoul, 120-749, Republic of Korea.
Park
Jae Won
JW
Sohn
Mi Jin
MJ
Lee
Seungwon
S
Oh
Jae Hoon
JH
Kim
Hyung Cheol
HC
Bahk
Young Yil
YY
Kim
Yu Sam
YS
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000097:blood; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D016780:Malaria, Vivax / Q000175:diagnosis*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000276:immunology* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000276:immunology; D015203:Reproducibility of Results; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D000913:Antibodies, Protozoan; D020066:Merozoite Surface Protein 1; D011993:Recombinant Fusion Proteins