title: Identification of a single genome by electron paramagnetic resonance (EPR) with nitroxide-labeled oligonucleotide probes.
abstract: Nitroxide-labeled nucleic acids are used as a molecular size sensor to identify as few as one genome under polymerase chain reaction (PCR) conditions by electron paramagnetic resonance (EPR) spectroscopy. DNA identification is based on differences in the EPR spectra of mononitroxide-labeled nucleic acids. The experimental data imply that rapid DNA identification can be achieved in many systems by EPR at the molecular level.
authors.affiliation: Department of Chemistry, University of Cincinnati, OH 45221-0172, USA.
authors.forename: Jeff D
authors.initials: JD
authors.lastname: Hester
authors.forename: Elizabeth V
authors.initials: EV
authors.lastname: Bobst
authors.forename: David D
authors.initials: DD
authors.lastname: Kryak
authors.forename: Albert M
authors.initials: AM
authors.lastname: Bobst
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D003497:Cyclic N-Oxides / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000032:analysis*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D016819:Encephalitozoon / Q000737:chemistry / Q000235:genetics*; D018503:Genome, Protozoan; D009589:Nitrogen Oxides / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet; D013113:Spin Labels
chemical_list: D003497:Cyclic N-Oxides; D017931:DNA Primers; D016054:DNA, Protozoan; D009589:Nitrogen Oxides; D015345:Oligonucleotide Probes; D013113:Spin Labels; C039900:nitroxyl