title: Effect of glycine and leucine on the recovery of frozen-thawed Lavandula vera cells.
abstract: Ammonium ion is known to inhibit the recovery of frozen-thawed Lavendula vera cells from freezing injury. In this study, we examined the effect of glycine and leucine on the recovery of frozen-thawed L. vera cells. Both amino acids show a more deleterious effect on the recovery of freeze-injured cells than ammonium ion according to the TTC assay of the post-thaw cultured cells. However, the deleterious effect differed in quality between glycine and leucine. Glycine not only inhibited the recovery of frozen-thawed cells but also promoted the lethal process of freezing injury. Although leucine did not promote the lethal process of freezing-injury, it did inhibit the recovery of freezing injury.
authors.forename: A
authors.initials: A
authors.lastname: Kuriyama
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Yamana