title: Ultra-rapid freezing and storage of rat embryos in an electric refrigerator at -130 degree C without liquid cryo-agents, with ultra-short exposure in the freezing medium and direct rehydration after thawing.
abstract: An ultra-rapid freezing technique for embryos has been developed. This procedure involves: ultra-short exposure to cryoprotectants; freezing of embryos in a metallic powder pre-cooled in an electric refrigerator at -130 degree C; storage of embryos ina refrigerator at -130 degree C; direct rehydration after thawing.
authors.affiliation: Kharkov Biotechnical Center, Kharkov, P.O.Kulinichi, 312120 Ukraine.
authors.forename: V V
authors.initials: VV
authors.lastname: Isachenko
authors.forename: J L
authors.initials: JL
authors.lastname: Alabart
authors.forename: E F
authors.initials: EF
authors.lastname: Isachenko
authors.forename: N D
authors.initials: ND
authors.lastname: Bezugly
authors.forename: H W
authors.initials: HW
authors.lastname: Michelmann