A new promoter for alpha1C subunit of human L-type cardiac calcium channel Ca(V)1.2.
The cardiac Ca channel known as alpha1C or Ca(V)1.2 is shown to express a new longer first exon equivalent to that formerly reported in rabbit heart or rat aorta. Ribonuclease protection assay indicates that this exon is found in the majority of Ca(V)1.2 transcripts in human heart RNA. The presence of this exon also suggests that expression of this transcript is driven by a promoter immediately upstream of this exon and its 5' untranslated region. The putative promoter exhibits 69% homology to its rat counterpart and displays functional promoter activity when transfected into heart cells in culture in luciferase-expressing constructs.
Dai
Bosong
B
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, TX 77555-0641, USA.
Saada
Nehad
N
Echetebu
Clement
C
Dettbarn
Christine
C
Palade
Philip
P
D020121:5' Untranslated Regions / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020746:Calcium Channels, L-Type / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D005091:Exons / Q000235:genetics; D006321:Heart / Q000502:physiology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms; D051381:Rats; D014162:Transfection
D020121:5' Untranslated Regions; D020746:Calcium Channels, L-Type; C419385:L-type calcium channel alpha(1C); D020033:Protein Isoforms