One repeat of the cell wall binding domain is sufficient for anchoring the Lactobacillus acidophilus surface layer protein.
The N-terminal repeat (SAC1) of the S-protein of Lactobacillus acidophilus bound efficiently and specifically to cell wall fragments (CWFs) when fused to green fluorescent protein, whereas the C-terminal repeat (SAC2) did not. Treatment of CWFs with hydrofluoric acid, but not phenol, prevented binding. Apparently, SAC1 is necessary and sufficient for cell wall binding. Our data suggest that SAC anchors the S-protein to a cell wall teichoic acid.
Smit
Egbert
E
TNO Nutrition and Food Research Institute, Department of Applied Microbiology and Gene Technology, 3700 AJ Zeist, The Netherlands.
Pouwels
Peter H
PH
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D004202:Disinfectants; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D019800:Phenol; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D013010:Sonication
D001426:Bacterial Proteins; D004202:Disinfectants; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C055764:S-layer proteins; D019800:Phenol