Green tea polyphenol blocks h(2)o(2)-induced interleukin-8 production from human alveolar epithelial cells.
Reactive oxygen species (ROS) play crucial roles in ischemia-reperfusion (IR) injury of lung transplants. Reactive oxygen species may stimulate the production of neutrophil chemotactic factors such as interleukin-8 (IL-8), from alveolar epithelial cells, causing recruitment and activation of neutrophils in the reperfused tissue. Green tea polyphenol has potent anti-oxidative activities and anti-inflammatory effects by decreasing cytokine production. In the present study, we found that green tea polyphenol significantly inhibited IL-8 production induced by hydrogen peroxide (H(2)O(2)) in human lung alveolar epithelial cells (A549 line). It has been shown that mitogen activated protein kinases, such as Jun N-terminal kinase (JNK), p38 and p44/42, could mediate IL-8 production from a variety of cell types. We further investigated the effect of green tea polyphenol on these protein kinases, and demonstrated that H(2)O(2)-induced phosphorylation of JNK and p38 but not p44/42 was inhibited by green tea polyphenol in A549 cells. We speculate that green tea polyphenol may inhibit H(2)O(2)-induced IL-8 production from A549 cells through inactivation of JNK and p38.
Matsuoka
Katsunari
K
Department of Thoracic Surgery, Kyoto University, Japan.
Isowa
Noritaka
N
Yoshimura
Takashi
T
Liu
Mingyao
M
Wada
Hiromi
H
D000975:Antioxidants / Q000494:pharmacology; D015153:Blotting, Western; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004847:Epithelial Cells / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism*; D005419:Flavonoids; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016209:Interleukin-8 / Q000096:biosynthesis*; D008168:Lung / Q000166:cytology* / Q000378:metabolism*; D010477:Perfusion; D010636:Phenols / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D059808:Polyphenols; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013662:Tea / Q000378:metabolism*; D013997:Time Factors
D000975:Antioxidants; D005419:Flavonoids; D016209:Interleukin-8; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D012333:RNA, Messenger; D013662:Tea; D006861:Hydrogen Peroxide