Short-term granulocyte colony-stimulating factor and erythropoietin treatment enhances hematopoiesis and survival in the mitomycin C-conditioned Fancc(-/-) mouse model, while long-term treatment is ineffective.
Transient treatment with cytokines appears to improve hematopoietic function in Fanconi anemia; however, the effectiveness or adverse effect of long-term treatment is not known. The mitomycin C-treated Fancc(-/-) mouse provides a valuable model to address long-term efficacy of such treatment. Fancc(-/-) mice injected with granulocyte colony-stimulating factor, erythropoietin, or both cytokines showed a delay in mitomycin C (MMC)-induced bone marrow (BM) failure compared to untreated mice. However, long-term cytokine exposure followed by MMC challenges did not protect mice from the reduction of peripheral blood counts or the number of early myeloid progenitors. These results suggest that cytokine treatment may be beneficial only in the short-term, while long-term treatment is not protective for BM aplasia.
Carreau
Madeleine
M
Unité de génétique humaine et moléculaire, CHUQ-Hôpital St-François d'Assise, Laval University, Québec, QC, Canada. madeleine.carreau@crsfa.ulaval.ca
Liu
Lili
L
Gan
Olga I
OI
Hitzler
Johann K
JK
Dick
John E
JE
Buchwald
Manuel
M
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D004906:Erythrocyte Count; D004921:Erythropoietin / Q000008:administration & dosage*; D005199:Fanconi Anemia / Q000188:drug therapy* / Q000401:mortality / Q000473:pathology; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000473:pathology; D007958:Leukocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D016685:Mitomycin / Q000008:administration & dosage*; D010198:Pancytopenia / Q000139:chemically induced; D015996:Survival Rate
D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; D016685:Mitomycin