Moderate physical exercise induces the oxidation of human blood protein thiols.
Exercise is known to induce the oxidation of blood low-molecular-weight (LMW) thiols such as reduced glutathione (GSH). We previously reported that full-marathon running induced a decrease in human plasma levels of protein-bound sulfhydryl groups (p-SHs). Moderate exercise, a 30-min running at the intensity of the individual ventilatory threshold, performed by untrained healthy females caused a significant decrease in erythrocyte levels of p-SHs (mostly hemoglobin cysteine residues) and LMW thiols, but their levels returned to each baseline by 2 h. No significant change in plasma LMW thiols was observed. However, plasma levels of p-SHs significantly decreased after running and remained unchanged after 24 h. These results suggest that moderate exercise causes the oxidation of blood thiols, especially protein-bound thiols.
Inayama
Takayo
T
National Institute of Health and Nutrition, Tokyo, Japan. tinayama@mail.cc.tohoku.ac.jp
Oka
Jun
J
Kashiba
Misato
M
Saito
Makoto
M
Higuchi
Mitsuru
M
Umegaki
Keizo
K
Yamamoto
Yorihiro
Y
Matsuda
Mitsuo
M
D000328:Adult; D015308:Anaerobic Threshold / Q000502:physiology; D001798:Blood Proteins / Q000378:metabolism*; D003402:Creatine Kinase / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D008970:Molecular Weight; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D001798:Blood Proteins; D013438:Sulfhydryl Compounds; D003402:Creatine Kinase