[Evaluating angiogenesis and osteogenesis modified by vascular endothelial growth factor (VEGF)].
OBJECTIVE
This intention of this study was to investigate the influence of controlled release of vascular endothelial growth factor (VEGF) on angiogenesis and osteogenesis in a mandibular defect model.


METHODS
A total of 56 rabbits were operated and bicortical holes were placed in the mandible. The defects were filled with collagen type I implants, collagen implants complexed with 0.8-microgram VEGF165, or left without any filling. After 3, 7, 14, and 28 days specimens were taken and histologic, histomorphometric, and immunohistologic analyses were carried out concerning density of vessels, total surface of vessels, bone surface, and bone density.


RESULTS
The number of vessels was increased in all groups up to 14 days, followed by physiologic regression in the control groups, whereas the study group showed persistently high numbers. The density of regenerated bone was significantly higher in the study group.


CONCLUSIONS
The activation of angiogenesis using VEGF165 leads to more intensive angiogenesis and bone regeneration.
Kleinheinz
J
J
Klinik und Poliklinik für Mund- und Kiefer-Gesichtschirurgie, Westfälische Wilhelms-Universität Münster, Waldeyerstrasse 30, 48129 Münster. Joklein@uni-muenster.de
Wiesmann
H P
HP
Stratmann
U
U
Joos
U
U
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D024042:Collagen Type I; D016228:Endothelial Growth Factors / Q000494:pharmacology*; D008222:Lymphokines / Q000494:pharmacology*; D008297:Male; D008334:Mandible / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D019919:Prosthesis Implantation; D011817:Rabbits; D013268:Stimulation, Chemical; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D024042:Collagen Type I; D016228:Endothelial Growth Factors; D008222:Lymphokines; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors