title: Preferential expression of RB1-inducible coiled-coil 1 in terminal differentiated musculoskeletal cells.
abstract: RB1-inducible coiled-coil 1 (RB1CC1) is a nuclear DNA-binding protein that can induce RB1 (retinoblastoma 1) expression. RB1CC1 is abundantly expressed in human musculoskeletal and cultured osteosarcoma cells. The present study analyzed the expression of RB1CC1 and RB1 in osteosarcoma cells and in musculoskeletal cells of human embryos to evaluate the contribution of both genes to the maturational process of musculoskeletal cells. The amount of RB1CC1 message was closely related to RB1 expression in various osteosarcoma cell lines. RB1CC1 expression was difficult to detect in immature proliferating chondroblasts or myogenic cells in human embryos, but became obvious and prominent concomitantly with the maturation of osteocytes, chondrocytes, and skeletal muscle cells. RB1CC1 expression in these musculoskeletal cells increased with RB1 expression, which is linked to the terminal differentiation of many tissues and cells. In addition, the introduction of wild-type RB1CC1 decreased the formation of macroscopic colonies in the cell growth assay. Accordingly, both RB1CC1 and RB1 genes preferentially co-expressed and contributed to the maturation of human embryonic musculoskeletal cells, and may regulate the proliferative activity and maturation of tumor cells derived from these tissues.
authors.affiliation: Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Otsu, Shiga, Japan. chano@belle.shiga-med.ac.jp
authors.forename: Tokuhiro
authors.initials: T
authors.lastname: Chano
authors.forename: Yukikazu
authors.initials: Y
authors.lastname: Saeki
authors.forename: Massimo
authors.initials: M
authors.lastname: Serra
authors.forename: Keiji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Okabe
mesh_terms: D000071183:Autophagy-Related Proteins; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009141:Musculoskeletal System / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D009379:Neoplasms, Muscle Tissue / Q000235:genetics / Q000473:pathology; D011247:Pregnancy; D011505:Protein-Tyrosine Kinases; D016160:Retinoblastoma Protein / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D000071183:Autophagy-Related Proteins; C407704:RB1CC1 protein, human; D016160:Retinoblastoma Protein; D011505:Protein-Tyrosine Kinases