title: Synthesis of ODNs containing 4-methylamino-1,8-naphthalimide as a fluorescence probe in DNA.
abstract: Synthesis and fluorescence properties of oligodeoxynucleotides containing 4-methylamino-1,8-naphthalimide (NI) have been described. NI was successfully incorporated into DNA without significant destabilization of DNA whilst retaining its high fluorescence quantum yield. The attachment site of the NI greatly affected its property as an energy acceptor in FRET analysis.
authors.affiliation: The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka, Japan. kiyohiko@sanken.osaka-u.ac.jp
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Kawai
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kawabata
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tojo
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Majima
mesh_terms: D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007094:Imides / Q000138:chemical synthesis*; D009281:Naphthalenes / Q000138:chemical synthesis*; D053644:Naphthalimides; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis
chemical_list: C475719:4-methylamino-1,8-naphthalimide; D005456:Fluorescent Dyes; D007094:Imides; D009281:Naphthalenes; D053644:Naphthalimides; D009838:Oligodeoxyribonucleotides; D004247:DNA