Effect of treatment with follicle-stimulating hormone or bovine somatotropin on the quality of oocytes aspirated in the autumn from previously heat-stressed cows.
Conception in dairy cows during autumn remains low even after summer temperatures decline. This is possibly a residual effect of heat stress on oocyte quality. Lactating Holsteins previously heat-stressed during summer were used in two experiments (n = 8 and 16 cows) in autumn to examine hormonal strategies for improving quality of oocytes. Follicles (3 to 8 mm in diameter) were aspirated by the ovum pick-up procedure on d 4 of two consecutive estrous cycles of treated and control cows. Oocytes were classified morphologically, matured in vitro, chemically activated, and cultured for 8 d. In Experiment 1, FSH (2 x 200 mg, at a 12-h interval) was injected on d 5 and 12 of cycle 1 (treated cycle). Before FSH, the percentage of good quality oocytes (grade I) and the cleavage rate averaged 52 and 24%, respectively, in both groups. During the FSH-treated cycle, more 6- to 9-mm follicles were observed. In the subsequent cycle, rates of grade I oocytes and cleavage were significantly higher in FSH-treated than in control cows (89 vs. 51% and 85 vs. 31%, respectively). In Experiment 2, recombinant bovine somatotropin (bST, 500 mg) was injected on d 4 and 18 of the treated cycle. The bST treatment increased the number of 3- to 5-mm follicles. Before bST, grade I (39%) and cleaved oocytes (40%), were similar across treatment groups. In the subsequent cycle, the percentage of grade I oocytes was significantly higher in treated than in control cows (72 vs. 26%), but cleavage rates were similar. Neither FSH or bST improved blastocyst formation, and regardless of treatment, few blastocysts were formed. Treatment with bST improved oocyte morphology, whereas FSH improved both oocyte morphology and cleavage rates during autumn following summer heat stress.
Roth
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Z
Department of Animal Science, Faculty of Agriculture, the Hebrew University, Rehovot, Israel. wolf@agri.huji.ac.il
Arav
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Braw-Tai
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R
Bor
A
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Wolfenson
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D
D000818:Animals; D002417:Cattle / Q000502:physiology*; D002418:Cattle Diseases / Q000188:drug therapy / Q000503:physiopathology; D005314:Embryonic and Fetal Development; D005260:Female; D005298:Fertility / Q000187:drug effects / Q000502:physiology*; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000494:pharmacology*; D013006:Growth Hormone / Q000494:pharmacology*; D018882:Heat Stress Disorders / Q000188:drug therapy / Q000503:physiopathology / Q000662:veterinary; D006358:Hot Temperature / Q000009:adverse effects*; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D011897:Random Allocation; D012621:Seasons; D013396:Suction; D013997:Time Factors
C073294:growth hormone, bovine; D005640:Follicle Stimulating Hormone; D013006:Growth Hormone