Comparison of Giemsa and acridine orange stains for the diagnosis of Leishmania donovani in biopsies of infected hamsters.
Identical impression smears of spleen, liver and bone marrow biopsy materials from Leishmania donovani-infected hamsters were stained using either acridine orange or Giemsa. Spleen parasite-loads calculated from the two stains for identical biopsy material were significantly different from each other. However, liver and bone marrow parasite- loads calculated from either Giemsa-stained or acridine orange-stained biopsies were not significantly different from each other. This study has shown that acridine orange, which is a quick and simple technique, has great potential in the diagnosis of kala-azar when liver and bone marrow biopsies are used.
Mbati
Peter A.
PA
Kenya Medial Research Institute, Biomedical Sciences Research Centre, P.O. Box 54840, Nairobi, Kenya.
Abok
Kisia
K
Anjili
Christopher O.
CO
Orago
Alloys/FIRST_NAME> S. Orago Jim S.
AS
Kagai
Jim M.
JM
Githure
John I.
JI
Koech
Davy K.
DK