title: Identification of a criminal by DNA typing in a rape case in Rio de Janeiro, Brazil.
abstract: BACKGROUND
  Human DNA identification is a powerful tool for paternity cases as well as for criminal investigation, in which biological evidence is typed after collection from crime scenes and for the identification of human remains.
  
  
  OBJECTIVE
  Identification of a criminal in a rape case with 4 suspects using STR and VNTR DNA analysis.
  
  
  METHODS
  Forensic DNA analysis.
  
  
  METHODS
  DNA Diagnostic Laboratory, Universidade Estadual do Rio de Janeiro, Brazil.
  
  
  METHODS
  Blood from 4 suspects and the victim, and skin from the fetus.
  
  
  METHODS
  Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
  
  
  RESULTS
  Three of the suspects were excluded and one of them was identified as the biological father of the fetus after typing with CTT and FFv Multiplexes. Complementary DNA typing at 3 VNTR loci was also carried out.
  
  
  CONCLUSIONS
  After typing four suspects using 6 STR loci, one of them was identified as the biological father of the fetus. In order to significantly enhance the Combined Paternity Index (PI), complementary DNA typing in 3 VNTR loci was carried out. The included suspect was found to be the biological father with a PI of 412,860 (Probability of Paternity: 99.9997%).
authors.affiliation: DNA Diagnostic Laboratory, Instituto de Biologia, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, Brazil. acgoes@uerj.br
authors.forename: Andréa Carla de Souza
authors.initials: AC
authors.lastname: Góes
authors.forename: Dayse Aparecida da
authors.initials: DA
authors.lastname: Silva
authors.forename: Cristiane Santana
authors.initials: CS
authors.lastname: Domingues
authors.forename: João Marreiro
authors.initials: JM
authors.lastname: Sobrinho
authors.forename: Elizeu Fagundes de
authors.initials: EF
authors.lastname: Carvalho
mesh_terms: D000293:Adolescent; D001938:Brazil; D002650:Child Abuse, Sexual; D016172:DNA Fingerprinting; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011902:Rape; D012091:Repetitive Sequences, Nucleic Acid