Nek11, a new member of the NIMA family of kinases, involved in DNA replication and genotoxic stress responses.
DNA replication and genotoxic stresses activate various checkpoint-associated protein kinases, and checkpoint dysfunction often leads to cell lethality. Here, we have identified new members of the mammalian NIMA family of kinases, termed Nek11L and Nek11S (NIMA-related kinase 11 Long and Short isoform) as novel DNA replication/damage stresses-responsive kinases. Molecular cloning and biochemical studies showed that the catalytic domain of Nek11 is most similar to Nek4 and Nek3, and substrate specificity of Nek11L is distinguishable from those of NIMA and Nek2. The expression of nek11L mRNA increased through S to G(2)/M phase, and subcellular localization of Nek11 protein altered between interphase and prometaphase, suggesting multiple roles of Nek11. We found an activation of Nek11 kinase activity when cells were treated with various DNA-damaging agents and replication inhibitors, and this activation of Nek11 was suppressed by caffeine in HeLaS3 cells. The transient expression of wild-type Nek11L enhanced the aphidicolin-induced S-phase arrest, whereas the aphidicolin-induced S-phase arrest was reduced in the U2OS cell lines expressing kinase-negative Nek11L (K61R), and these cells were more sensitive to aphidicolin-induced cell lethality. Collectively, these results suggest that Nek11 has a role in the S-phase checkpoint downstream of the caffeine-sensitive pathway.
Noguchi
Kohji
K
Department of Bioactive Molecules, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Fukazawa
Hidesuke
H
Murakami
Yuko
Y
Uehara
Yoshimasa
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002110:Caffeine / Q000494:pharmacology; D020134:Catalytic Domain; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D002460:Cell Line; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis*; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D020224:Expressed Sequence Tags; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D007438:Introns; D008164:Luminescent Proteins / Q000378:metabolism; D008677:Metaphase; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D000072380:NIMA-Related Kinase 1; D000072379:NIMA-Related Kinases; D010766:Phosphorylation; D010802:Phylogeny; D011233:Precipitin Tests; D011485:Protein Binding; D020033:Protein Isoforms; D011494:Protein Kinases / Q000737:chemistry* / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000502:physiology*; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D016196:S Phase; D017386:Sequence Homology, Amino Acid; D012984:Software; D013379:Substrate Specificity; D013997:Time Factors; D014407:Tumor Cells, Cultured
D002110:Caffeine; D018797:Cell Cycle Proteins; D000697:Central Nervous System Stimulants; D004247:DNA; D018076:DNA, Complementary; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000072380:NIMA-Related Kinase 1; D000072379:NIMA-Related Kinases; D020033:Protein Isoforms; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D012313:RNA; C000606914:NEK1 protein, human; C469355:NEK11 protein, human