Biological effects of rutin on the survival of Escherichia coli AB1157 and on the electrophoretic mobility of plasmid PUC 9.1 DNA.
The use of natural products as medicines is growing in the world. The rutin, a compound isolated from Ruta graveolens, is a flavonoid, which has been suggested to have antioxidant properties and to reduce the triacylglycerol levels. In this study, plasmid desoxyribonucleic acid (DNA) was exposed to rutin (0.33, 10, 20, 30 microg/ml) in presence of stannous chloride (SnCl2), a reducing agent widely used to obtain radiopharmaceuticals labeled with technetium-99m. Samples of the plasmid DNA were analyzed through agarose gel electrophoresis. E. coli AB1157 culture was also incubated with rutin (3, 30, 50, 100 microg/ml) and the survival fractions were calculated. The results show that the rutin, in these concentrations, is not capable of: i/ damaging the DNA, ii/ protecting the DNA from the SnCl2 redox action, and iii/ inactivating the E. coli AB1157 culture. The analysis of our data indicates that rutin do not present toxic activity in the evaluated systems.
Bernardo
Luciana C
LC
Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofisica e Biometria, RJ, Brazil. bernardo@uerj.br
de Oliveira
Márcia B N
MB
da Silva
Cláudia R
CR
Dantas
Flávio J S
FJ
de Matros
José C P
JC
Caldeira-de-Araújo
Adriano
A
Moura
Roberto S
RS
Bernardo-Filho
Mario
M
D000975:Antioxidants / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects; D024202:Electrophoretic Mobility Shift Assay; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D010957:Plasmids / Q000187:drug effects*; D032105:Ruta / Q000737:chemistry; D012431:Rutin / Q000494:pharmacology*; D017971:Tin Compounds
D000975:Antioxidants; D017971:Tin Compounds; C023599:stannous chloride; D012431:Rutin