title: Strain variation in Mediterranean and Red Sea Mycobacterium marinum isolates.
abstract: Four different PCR fingerprinting techniques were tested to distinguish possible strain variations in fourteen Mycobacterium marinum isolates, thirteen from Mediterranean and Red Sea fishes and one from a patient in Sardinia, Italy. PCR ribotyping and ERIC (enterobacterial repetitive consensus sequences)-PCR were found to be non-discriminative, whereas IS (insertion sequences)-PCR and GTG (GTG sequences repeats)-PCR could distinguish the clinical isolate from the piscine isolates, two Italian piscine isolates from all other isolates, but not the Greek isolates from the Israeli isolates. Our results indicate that GTG-PCR and IS-PCR have superior discriminative properties and are thus useful molecular tools for epidemiological studies of M. marinum.
authors.affiliation: Dipartimento di Scienze Biomediche, Università degli studi di Sassari, Italy.
authors.forename: L A
authors.initials: LA
authors.lastname: Sechi
authors.forename: A
authors.initials: A
authors.lastname: Colorni
authors.forename: I
authors.initials: I
authors.lastname: Duprè
authors.forename: P
authors.initials: P
authors.lastname: Molicotti
authors.forename: G
authors.initials: G
authors.lastname: Fadda
authors.forename: S
authors.initials: S
authors.lastname: Zanetti
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D018834:Chaperonin 60; D018833:Chaperonins / Q000737:chemistry / Q000235:genetics; D016384:Consensus Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology*; D014644:Genetic Variation; D006801:Humans; D019156:Indian Ocean; D007558:Italy; D008522:Mediterranean Sea; D009164:Mycobacterium Infections / Q000382:microbiology* / Q000662:veterinary; D019910:Mycobacterium marinum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010473:Perciformes; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D021521:Ribotyping
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins