title: Production of recombinant human calcitonin from silkworm (B. mori) larvae infected by baculovirus.
abstract: A synthetic modified gene encoding the human calcitonin analog (hmCT) was expressed by use of the baculovirus expression system. After injection with recombinant baculoviruses, the hmCT-GST fusion protein was produced within the silkworm larvae. The fusion protein was purified by affinity chromatography. Biological activity of hmCT for hypercalcemic effect was determined in normal rats.
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Science, Shanghai, 200031, China.
authors.forename: Guan-Zhen
authors.initials: GZ
authors.lastname: Yang
authors.forename: Zhen-zhen
authors.initials: ZZ
authors.lastname: Chen
authors.forename: Da-Fu
authors.initials: DF
authors.lastname: Cui
authors.forename: Bo-liang
authors.initials: BL
authors.lastname: Li
authors.forename: Xiang-fu
authors.initials: XF
authors.lastname: Wu
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000502:physiology*; D012831:Bombyx / Q000235:genetics / Q000502:physiology* / Q000821:virology; D002116:Calcitonin / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D017930:Genes, Reporter; D006458:Hemolymph / Q000737:chemistry; D006801:Humans; D006934:Hypercalcemia / Q000139:chemically induced; D007814:Larva / Q000502:physiology; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology
chemical_list: D011993:Recombinant Fusion Proteins; D002116:Calcitonin