title: Establishment of the telencephalon during gastrulation by local antagonism of Wnt signaling.
abstract: Cells at the anterior boundary of the neural plate (ANB) can induce telencephalic gene expression when transplanted to more posterior regions. Here, we identify a secreted Frizzled-related Wnt antagonist, Tlc, that is expressed in ANB cells and can cell nonautonomously promote telencephalic gene expression in a concentration-dependent manner. Moreover, abrogation of Tlc function compromises telencephalic development. We also identify Wnt8b as a locally acting modulator of regional fate in the anterior neural plate and a likely target for antagonism by Tlc. Finally, we show that tlc expression is regulated by signals that establish early antero-posterior and dorso-ventral ectodermal pattern. From these studies, we propose that local antagonism of Wnt activity within the anterior ectoderm is required to establish the telencephalon.
authors.affiliation: MRC Centre for Developmental Neurobiology, New Hunt's House, King's College London, SE1 9RT, London, United Kingdom. corinne.houart@kcl.ac.uk
authors.forename: Corinne
authors.initials: C
authors.lastname: Houart
authors.forename: Luca
authors.initials: L
authors.lastname: Caneparo
authors.forename: Carl
authors.initials: C
authors.lastname: Heisenberg
authors.forename: K
authors.initials: K
authors.lastname: Barth
authors.forename: Masaya
authors.initials: M
authors.lastname: Take-Uchi
authors.forename: Stephen
authors.initials: S
authors.lastname: Wilson
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D003598:Cytoskeletal Proteins; D003714:Denervation; D005775:Gastrula / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010802:Phylogeny; D016548:Prosencephalon / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D013687:Telencephalon / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D016212:Transforming Growth Factor beta; D051153:Wnt Proteins; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000302:isolation & purification*; D000099274:Frizzled-Related Proteins
chemical_list: D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D003598:Cytoskeletal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D016212:Transforming Growth Factor beta; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D000099274:Frizzled-Related Proteins; C525826:bmp7a protein, zebrafish; C094521:wnt8a protein, zebrafish; C094522:wnt8b protein, zebrafish