title: Phase-change related epigenetic and physiological changes in Pinus radiata D. Don.
abstract: DNA methylation and polyamine levels were analysed before and after Pinus radiata D. Don. phase change in order to identify possible molecular and physiological phase markers. Juvenile individuals (without reproductive ability) were characterised by a degree of DNA methylation of 30-35% and a ratio of free polyamines to perchloric acid-soluble polyamine conjugates greater than 1, while mature trees (with reproductive ability) had 60% 5-methylcytosine and a ratio of free polyamines to perchloric acid-soluble polyamine conjugates of less than 1. Results obtained with trees that attained reproductive capacity during the experimental period confirmed that changes in the degree of DNA methylation and polyamine concentrations found among juvenile and mature states come about immediately after the phase change. We suggest that both indicators may be associated with the loss of morphogenic ability during ageing, particularly after phase change, through a number of molecular interactions, which are subsequently discussed.
authors.affiliation: Laboratorio Fisiología Vegetal, Departmento Biología de Organismos y Sistemas, Universidad de Oviedo, Spain.
authors.forename: Mario F
authors.initials: MF
authors.lastname: Fraga
authors.forename: Maria Jesús
authors.initials: MJ
authors.lastname: Cañal
authors.forename: Roberto
authors.initials: R
authors.lastname: Rodríguez
mesh_terms: D019175:DNA Methylation; D028223:Pinus / Q000235:genetics* / Q000502:physiology; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013997:Time Factors
chemical_list: D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine