title: Evaluation of antibody response to canine coronavirus infection in dogs by Western Blotting analysis.
abstract: We investigated by Western Blotting the antibody responses against the three major structural proteins of Canine coronavirus (CCoV) in dogs naturally infected. A pool of Elisa positive sera were also tested to clearly identify the binding profiles of CCoV proteins. The immune response to S protein was barely detectable in naturally infected dogs, whereas anti-M and anti-N antibodies were detected with a very strong reaction and for a long time post infection. The limited response to S protein may explain the poor protection of dogs and the possibility of persisting infection.
authors.affiliation: Department of Health and Animal Well-being, Faculty of Veterinary Medicine, Bari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Elia
authors.forename: N
authors.initials: N
authors.lastname: Decaro
authors.forename: A
authors.initials: A
authors.lastname: Tinelli
authors.forename: V
authors.initials: V
authors.lastname: Martella
authors.forename: A
authors.initials: A
authors.lastname: Pratelli
authors.forename: C
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D015153:Blotting, Western / Q000662:veterinary; D018352:Coronavirus Infections / Q000276:immunology / Q000662:veterinary*; D017939:Coronavirus, Canine / Q000276:immunology / Q000378:metabolism*; D004283:Dog Diseases / Q000276:immunology / Q000821:virology*; D004285:Dogs; D005767:Gastrointestinal Diseases / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D015678:Viral Structural Proteins / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral; D015678:Viral Structural Proteins