title: The systemic cellular immune response in the Helicobacter pylori-associated duodenal ulcer and chronic antral gastritis.
abstract: OBJECTIVE
  The exact pathogenesis of Helicobacter pylori infection is not fully understood. This study aims to evaluate the specific subset composition of peripheral blood lymphocytes in patients with H. pylori-positive duodenal ulcer n = 14), chronic antral gastritis n = 28), since reports so far have led to inconclusive and conflicting results.
  
  
  METHODS
  42 patients with dyspepsia and 50 controls underwent the following procedures: 1) gastroscopy and gastric biopsy (five specimens) 2) histology, 3) serologic test for anti-H. pylori antibodies IgG (Pyloriset EIA-G, Orion Diagnostica) and anticytotoxin associated gene A (cag A) IgG antibodies (VIVA Diagnositika by ELISA), 4) analysis of the peripheral blood lymphocytes using monoclonal antibodies reacting with lymphocyte cell surface antigens (anti-CD3, anti-CD19, anti-CD4, anti-CD8, anti-CD16 + CD56, anti-HLA DR) by flow-cytometry (Becton-Dickinson) to detect possible changes in the lymphocytes subpopulations in patients with duodenal ulcer and chronic antral gastritis.
  
  
  RESULTS
  We found no alteration in total T and B lymphocytes and CD4+ T, CD8+ T lymphocytes and natural killer cells of both duodenal ulcer and chronic antral gastritis patients compared to normal persons. Although there was a slight increase in the proportion of active T lymphocytes in duodenal ulcer and chronic antral gastritis groups comparing to healthy subjects the difference was not statistically significant.
  
  
  CONCLUSIONS
  These data indicate that there is no systemic alteration in the specific immune system in response to H. pylori in patients with duodenal ulcer and chronic antral gastritis.
authors.affiliation: Department of Gastroenterology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Yuceyar
authors.forename: Murat
authors.initials: M
authors.lastname: Saruc
authors.forename: Ali
authors.initials: A
authors.lastname: Kokuludag
authors.forename: Ender
authors.initials: E
authors.lastname: Terzioglu
authors.forename: Gamze
authors.initials: G
authors.lastname: Goksel
authors.forename: Aydin
authors.initials: A
authors.lastname: Isisag
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D002908:Chronic Disease; D004381:Duodenal Ulcer / Q000276:immunology*; D005260:Female; D005756:Gastritis / Q000276:immunology*; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D016130:Immunophenotyping; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011706:Pyloric Antrum / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial