5-propynyluracil.diaminopurine: an efficient base-pair for non-enzymatic transcription of DNA.
The Up.D base-pair (5-propynyl uracil.diaminopurine) is found to be more effective at non-enzymatic transcription than the corresponding natural T.A pair; under non-enzymatic reaction conditions where the natural T.A base-pair fails, a DNA template bearing Up efficiently directs the incorporation of D into a product RNA strand.
Chaput
John C
JC
Department of Chemistry, University of California, Riverside, CA 92521, USA.
Sinha
Surajit
S
Switzer
Christopher
C
D015075:2-Aminopurine / Q000031:analogs & derivatives* / Q000737:chemistry*; D000225:Adenine / Q000737:chemistry; D000480:Alkynes / Q000737:chemistry*; D001345:Autoradiography; D020029:Base Pairing; D004261:DNA Replication; D004591:Electrophoresis, Polyacrylamide Gel; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry*
D000480:Alkynes; C083257:5-propynyluracil; D015075:2-Aminopurine; C007300:2,6-diaminopurine; D014498:Uracil; D000225:Adenine