Role of trichrome staining techniques in the diagnosis of intestinal parasitic infections.
OBJECTIVE
To compare the diagnostic yield of saline and iodine preparation with trichrome staining of fresh sample of stool for diagnosis of parasitic infection.


METHODS
Stools were examined in saline and iodine preparation and trichrome stained smears prepared according to the method recommended by WHO in oil immersion.


RESULTS
Out of 400 specimens 215 were positive for parasitic infection and of these 81 were diagnosed exclusively on trichrome stained samples.


CONCLUSIONS
Because of its better diagnostic field trichrome staining technique should be included in the routine stool examination.
Shoaib
S
S
Department of Pathology, Dow Medical College and Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi.
Hafiz
A
A
Tauheed
S
S
D001391:Azo Compounds; D004396:Coloring Agents; D003938:Diagnostic Techniques, Digestive System; D003967:Diarrhea / Q000469:parasitology; D004801:Eosine Yellowish-(YS); D005243:Feces / Q000469:parasitology; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000150:complications / Q000175:diagnosis*; D008739:Methyl Green; D012680:Sensitivity and Specificity
D001391:Azo Compounds; D004396:Coloring Agents; C017768:trichrome stain; D008739:Methyl Green; D004801:Eosine Yellowish-(YS)