title: Identification and characterization of a new family of C-type lectin-like genes from planaria Girardia tigrina.
abstract: A novel family of C-type lectin-like genes, denoted multidomain free lectin (MDFL), was identified in the freshwater planaria Girardia (Dugesia) tigrina. We cloned several genes that encode proteins comprising a signal peptide and a number of consecutive C-type lectin-like domains (CTLDs) interconnected by short linker stretches. Analyses of genomic organization, CTLD amino acid sequences, and the overall architecture of these proteins indicate that planarian proteins are a separate family of C-type lectin-like proteins. These genes are expressed in specifically differentiated gland cells of planaria and the corresponding proteins are excreted as components of the planarian body surface mucus.
authors.affiliation: Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, 117871 Moscow, Russia.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Shagin
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Barsova
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Bogdanova
authors.forename: Olga V
authors.initials: OV
authors.lastname: Britanova
authors.forename: Nadia
authors.initials: N
authors.lastname: Gurskaya
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Lukyanov
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Matz
authors.forename: Natalia I
authors.initials: NI
authors.lastname: Punkova
authors.forename: Natalia Y
authors.initials: NY
authors.lastname: Usman
authors.forename: Eugene P
authors.initials: EP
authors.lastname: Kopantzev
authors.forename: Emili
authors.initials: E
authors.lastname: Salo
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Lukyanov
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D017124:Conserved Sequence; D005091:Exons; D015801:Helminth Proteins / Q000737:chemistry; D007438:Introns; D037181:Lectins, C-Type / Q000737:chemistry / Q000235:genetics*; D008870:Microtubules / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009093:Mucus / Q000378:metabolism; D010802:Phylogeny; D010932:Planarians / Q000235:genetics* / Q000648:ultrastructure; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D015801:Helminth Proteins; D037181:Lectins, C-Type; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C111231:scarf protein, Dugesia tigrina; D002118:Calcium