Genome-wide transcriptional profiling in a histidine kinase mutant of Helicobacter pylori identifies members of a regulon.
To identify putative members of a regulon controlled by the H. pylori sensory histidine kinase HP0164 (HK0164), we constructed HK0164 null mutant H. pylori strains and analyzed bacterial gene transcription using DNA arrays. Seven genes were differentially expressed in multiple HK0164 mutant strains compared to their expression in control strains. Strain-dependent variations in differential expression were also detected. These results indicate that the signal transduction circuit utilizing HK0164 controls the transcription of at least seven genes in H. pylori.
Forsyth
Mark H
MH
Department of Biology, The College of William and Mary, Williamsburg, Virginia 23187, USA. mhfors@wm.edu
Cao
Ping
P
Garcia
Preston P
PP
Hall
Joshua D
JD
Cover
Timothy L
TL
D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D016680:Genome, Bacterial; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics*; D000071677:Histidine Kinase; D009154:Mutation / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D011494:Protein Kinases / Q000235:genetics*; D018085:Regulon / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
D011494:Protein Kinases; D000071677:Histidine Kinase