The adaptation of two similar soils to pyrene catabolism.
The development of pyrene catabolic activity was assessed in two similar soils (pasture and woodland) amended with 100 mg pyrene kg(-1) In the pasture and woodland soils, significant mineralisation of 14C-pyrene was observed after 8 and 76 weeks soil-pyrene contact times, respectively. In both soils, there were significant decreases (P<0.05) in the lag times and significant increases (P <0.05) in the maximum rates and extents of 14C-pyrene mineralised with increasing soil-pyrene contact time. A microbial inoculum was added to the woodland soil to assess if the previously added, but undegraded 14C-pyrene was bioavailable at 16 and 24 weeks. This resulted in the immediate mineralisation of the previously added 14C-pyrene, indicating that it was bioavailable but that the microbial community in the woodland soil had not developed the ability to mineralise pyrene. The relative contributions of the indigenous microflora to 14C-pyrene mineralisation were assessed by the addition of celective inhibitors, with bacteria seeming to be responsible for the mineralisation of pyrene in both soils. It is suggested that the rate of pyrene-transfer from the soil to the microorganisms was lower in the woodland soil due to its higher organic matter content.
Macleod
Christopher J A
CJ
Department of Environmental Science, Institute of Environmental and Natural Sciences, Lancaster University, UK.
Semple
Kirk T
KT
D000222:Adaptation, Physiological; D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D001682:Biological Availability; D002250:Carbon Radioisotopes; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D017753:Ecosystem; D011721:Pyrenes / Q000737:chemistry*; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D012989:Soil Pollutants
D002250:Carbon Radioisotopes; D011721:Pyrenes; D012987:Soil; D012989:Soil Pollutants; C030984:pyrene