Purification and Characterization of A Phospholipase A(2) from Agkistrodon blomhoffii Ussurensis of Changbai Mountains.
PLA(2)(EC3.1.1.4) of Agkistrodon blomhoffii Ussurensis of Changbai Mountain has been purified to homogeneity via four steps consisting of DEAE-Sephadex A-50, and three times Sephadex G-75 column chromatography in series. Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization, time of flight mass spectrometry, the PLA(2) was found to have a molecular weight of (14.008+/-0.007) kD. The optimal pH of the PLA(2) is in the range from 8.0 to 9.0, the optimal temperature is 45 degrees. Polymer is found in solution by MALDI/TOF/MS.
Sun
Ming-Zhong
MZ
Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, the Chinese Academy of Sciences, Changchun 130022, China. dqzhao@ns.ciac.jl.cn
Ding
Lan
L
Zhao
Da-Qing
DQ
Ni
Jia-Zan
JZ