title: Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter.
abstract: The orchid DSCKX1 is a new member of the cytokinin oxidase gene family, which catalyses the degradation of cytokinins bearing unsaturated isoprenoid side chains. A 3.7 kb fragment upstream of the DSCKX1 coding region was isolated, sequenced and characterized by deletion analysis of DSCKX1::beta-glucuronidase gene fusions using transient orchid and stable Arabidopsis transformation systems. Functional analysis of 5' deletions defined the 5'-upstream region that directs the expression in distinct tissues. Regulatory elements affecting the cytokinin induction of the DSCKX1 gene have also been delineated
authors.affiliation: Plant Growth and Development Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Republic of Singapore.
authors.forename: Shu Hua
authors.initials: SH
authors.lastname: Yang
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.forename: Chong Jin
authors.initials: CJ
authors.lastname: Goh
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D003583:Cytokinins / Q000494:pharmacology; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000201:enzymology* / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification; D018514:Plant Structures / Q000187:drug effects / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion
chemical_list: D003583:Cytokinins; D018744:DNA, Plant; D011993:Recombinant Fusion Proteins; D010088:Oxidoreductases; C019984:cytokinin oxidase; D005966:Glucuronidase