Biophysical evidence of lipid and carbohydrate binding activities of shrimp high density lipoprotein/beta glucan binding protein.
Crustacean High Density Lipoprotein/beta-Glucan Binding Protein (HDL/BGBP) has been studied due to its role in nutrition and immune response via activation of the defense cells (hemocytes) upon binding 1,3-D-beta-glucan carbohydrates. In this study, HDL/BGBP was found to be composed mainly of beta sheets, as determined by circular dichroism. Lipoprotein aggregation resulted when HDL/BGBP interacted with phospolipid vesicles, laminaribiose (1,3-beta-glucan disaccharide) or heparin. HDL/BGBP has similar dissociation constants for laminaribiose (K(d)=22 mM) or heparin (K(d)=46 mM) as determined by 90 degrees light scattering.
Vargas-Requena
Claudia L
CL
Marine Biotechnology Laboratory, Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo, Sonora, 83000, México.
Hernández-Santoyo
Alejandra
A
Yepiz-Plascencia
Gloria
G
Vargas-Albores
Francisco
F
Higuera-Ciapara
Inocencio
I
Rodríguez-Romero
Adela
A
Soulages
José L
JL
Sotelo-Mundo
Rogerio R
RR
D000818:Animals; D050260:Carbohydrate Metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D033364:Decapoda / Q000737:chemistry / Q000502:physiology*; D006458:Hemolymph / Q000737:chemistry; D006493:Heparin / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D017433:Protein Structure, Secondary
D002352:Carrier Proteins; D008077:Lipoproteins, LDL; D008081:Liposomes; D006493:Heparin