[Study of immobilization of Aspergillus oryzae mycelium aided by artificial neural network].
Aspergillus oryzae mycelium pellets with abundant aminoacylase were cultured by liquid fermentation. Mycelium pellets were immobilized by crosslinking method with reagent of gelatin and formaldehyde. Basing on the orthogonal design table L16 (4(5)), artificial neural network(ANN) model with back-propagation(BP) of error structured 4-10-15-1 was used to optimize the immobilization conditions. And the optimal conditions were obtained. Then the activity of the immobilized cells prepared under the optimal conditions was assayed. Compared with the free mycelium activity of 1800 u, the residual activity rate was 83%, which was in good accordance with that predicted by ANN. It illustrated that artificial neural network can be used to find optimal conditions for cell immobilization.
Wang
Yan
Y
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Zhang
Feng-Bao
FB
Gao
Zhi-Ming
ZM
Song
Zheng-Xiao
ZX
Zhang
Guo-Liang
GL
Zhao
Lin
L
D000581:Amidohydrolases / Q000187:drug effects* / Q000378:metabolism; D001236:Aspergillus oryzae / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004800:Enzymes, Immobilized; D005557:Formaldehyde / Q000494:pharmacology; D005780:Gelatin / Q000494:pharmacology; D025282:Mycelium; D016571:Neural Networks, Computer
D004800:Enzymes, Immobilized; D005557:Formaldehyde; D005780:Gelatin; D000581:Amidohydrolases; C019672:aminoacylase I