Effects of atrial natriuretic peptide on DNA synthesis and NADPH diaphorase activity in epitheliocytes and smooth muscle cells of the respiratory tract in newborn albino rats.
Newborn rats received single intraperitoneal injections of atrial natriuretic peptide (1-28) in a dose of 3.2x10(-8) mol/kg on day 6 of life. Autoradiography with 3H-thymidine showed that the peptide inhibited DNA synthesis in smooth muscle cells of the respiratory tract. Pretreatment with nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester attenuated, but did not abolish the effect of atrial natriuretic peptide (1-28). Histochemical assay for NADPH diaphorase showed that nitric oxide constitutively produced in the epithelium is involved in the growth-inhibitory effect of atrial natriuretic peptide (1-28) on proliferating smooth muscle cells.
Lebed'ko
O A
OA
Institute of Maternity and Child Welfare, Khabarovsk Branch, Far-Eastern Research Center for Physiology and Pathology of Respiration, Siberian Division of the Russian Academy of Medical Sciences, Far-Eastern State Medical University, Khabarovsk.
Timoshin
S S
SS
Tsygankov
V I
VI
D000818:Animals; D000831:Animals, Newborn; D009320:Atrial Natriuretic Factor / Q000378:metabolism* / Q000494:pharmacology; D001345:Autoradiography; D001980:Bronchi / Q000201:enzymology / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004848:Epithelium / Q000201:enzymology / Q000378:metabolism*; D009130:Muscle, Smooth / Q000201:enzymology / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D051381:Rats; D014132:Trachea / Q000201:enzymology / Q000378:metabolism*
D004791:Enzyme Inhibitors; D009320:Atrial Natriuretic Factor; D004247:DNA; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase; D019331:NG-Nitroarginine Methyl Ester