Cloning and Characterization of Mouse Glial Cell Line-derived Neurotrophic Growth Factor (GDNF).
We have cloned two unique forms of glial cell line-derived neurotrophic growth factor transcripts (GDNF-alpha and GDNF-beta) from adult mouse brain. The GDNF-beta form is similar to GDNF-alpha expect for a 78 bp deletion in the ORF. The deduced amino acid sequences of the mouse GDNF-alpha are highly homologous to those of rat and human GDNF. Reverse-transcription PCR from various mouse tissues showed extensive expression of both forms of GDNF. GDNF-alpha is coded by at least two distinct exons. The 3apos-UTR is entirely encoded in a single exon.
Wang
Fu-Jun
FJ
Department of Applied Biology, East China University of Science and Technology, Shanghai 200237, China. wfj@npc.haplink.com.cn, wfj@public1.sta.net.cn
Zhao
Jian
J
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Too
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