The signal transducer P(II) and bicarbonate acquisition in Prochlorococcus marinus PCC 9511, a marine cyanobacterium naturally deficient in nitrate and nitrite assimilation.
The amino acid sequence of the signal transducer P(II) (GlnB) of the oceanic photosynthetic prokaryote Prochlorococcus marinus strain PCC 9511 displays a typical cyanobacterial signature and is phylogenetically related to all known cyanobacterial glnB genes, but forms a distinct subclade with two other marine cyanobacteria. P(II) of P. marinus was not phosphorylated under the conditions tested, despite its highly conserved primary amino acid sequence, including the seryl residue at position 49, the site for the phosphorylation of the protein in the cyanobacterium Synechococcus PCC 7942. Moreover, P. marinus lacks nitrate and nitrite reductase activities and does not take up nitrate and nitrite. This strain, however, expresses a low- and a high-affinity transport system for inorganic carbon (C(i); K(m,app) 240 and 4 micro M, respectively), a result consistent with the unphosphorylated form of P(II) acting as a sensor for the control of C(i) acquisition, as proposed for the cyanobacterium Synechocystis PCC 6803. The present data are discussed in relation to the genetic information provided by the P. marinus MED4 genome sequence.
Palinska
Katarzyna A
KA
Unité; des Cyanobacté;ries, CNRS URA 2172, Département de Microbiologie Fondamentale et Mé;dicale, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France1.
Laloui
Wassila
W
Unité; des Cyanobacté;ries, CNRS URA 2172, Département de Microbiologie Fondamentale et Mé;dicale, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France1.
Bédu
Sylvie
S
Laboratoire de Chimie Bacté;rienne, CNRS, 31 chemin Joseph Aiguier, BP71 13277, Marseille Cedex 9, France2.
Loiseaux-de Goer
Susan
S
Station Biologique de Roscoff, CNRS UMR 1931, 29682 Roscoff Cedex, France3.
Castets
Anne Marie
AM
Unité; des Cyanobacté;ries, CNRS URA 2172, Département de Microbiologie Fondamentale et Mé;dicale, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France1.
Rippka
Rosmarie
R
Unité; des Cyanobacté;ries, CNRS URA 2172, Département de Microbiologie Fondamentale et Mé;dicale, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France1.
Tandeau de Marsac
Nicole
N
Unité; des Cyanobacté;ries, CNRS URA 2172, Département de Microbiologie Fondamentale et Mé;dicale, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France1.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001639:Bicarbonates / Q000378:metabolism*; D001692:Biological Transport; D000458:Cyanobacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008386:Marine Biology; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D051922:PII Nitrogen Regulatory Proteins; D010766:Phosphorylation; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014871:Water Microbiology
D001426:Bacterial Proteins; D001639:Bicarbonates; D009566:Nitrates; D009573:Nitrites; D051922:PII Nitrogen Regulatory Proteins; C404143:PIID regulatory protein, Bacteria