Differential and Tissue-specific Expression of Mitochondrial Elongation Factor Tu and Ts during Development.
The mitochondrial elongation factors Tu and Ts (EF-Tu(mt) and EF-Ts(mt)) are nuclear gene-encoded mitochondrial proteins and play important roles in growth and development. We prepared antibodies against EF-Tu(mt) and EF-Ts(mt) using purified recombinant EF-Tu(mt) and EF-Ts(mt). Western analysis indicates EF-Tu(mt) and EF-Ts(mt) are expressed differently in various tissues. Their expression in heart and liver increased during development and reached plateau at maturation. Expression of EF-Tu(mt) and EF-Ts(mt) exhi-bited similar tissue-specificity and changed synchronically during development. These results suggest that expression levels of EF-Tu(mt) and EF-Ts(mt) may be regulated by a similar mechanism related to the metabolic state of the cells.
Chen
Li
L
State Key Laboratory of Medical Neurobiology, Shanghai Medical University, Shanghai 200032, China. lanma@shmu.edu.cn
Xu
Qing
Q
Liu
Xu-Lei
XL
Zhao
Jing
J
Ma
Lan
L