[Induced-effects by additional taxol in suspension cultures of Taxus cuspidata cells].
Apoptosis was induced by taxol treatment in suspension cultures of Taxus cuspidata cells. Differential display technique was used to investigate the induced-gene expression between the taxol-induced T. cuspidata cells and normal control. Eight different expressed cDNA fragments were cloned and sequenced. These differential expressed fragments were further confirmed by Northern blotting hybridization with their original total RNAs. The result showed that three of the cDNA fragments were from control RNA and five of those were from taxol-induced T. cuspidata cells. The homology of the sequences revealed that one of the clones had 86% homology with ABA-responsive protein gene sequence in Arabidopsis thaliana, two of the clones had 50% homology with endochitinase precursor in tomato and the other 5 clones, which might be new gene fragments, had no significant homology with the known gene sequences in GenBank/EMBL/DDBJ.
Ma
Zhen-Yi
ZY
Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Wang
Yan-Dong
YD
Yuan
Ying-Jin
YJ
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods; D002478:Cells, Cultured; D018744:DNA, Plant; D008969:Molecular Sequence Data; D017239:Paclitaxel / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D020946:Taxus / Q000166:cytology / Q000235:genetics*
D000972:Antineoplastic Agents, Phytogenic; D018744:DNA, Plant; D017239:Paclitaxel