The first isolation of Nocardia veterana from a human mycetoma.
The clinical isolates from biopsy specimen human subcutaneous nodule developed orange-colored and wrinkled colonies on Sabouraud's dextrose agar at 24 C for 2 weeks. The isolates were aerobic and gram-positive. The bacteria were rod-shaped to coccoid and 1 x 5 microm in size. The assimilation tests revealed that the clinical isolate was identical to a reference strain of Nocardia veterana. A nucleotide sequence analysis of the 16S ribosomal DNA from the isolate and a reference strain of N. veterana showed 99.8% similarity. All data are consistent with the conclusion that the isolate in this human case of mycetoma is N. veterana.
Kano
Rui
R
Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan. kano@brs.nihon-u.ac.jp
Hattori
Yousuke
Y
Murakami
Noboru
N
Mine
Naoko
N
Kashima
Masato
M
Kroppenstedt
Reiner M
RM
Mizoguchi
Masako
M
Hasegawa
Atsuhiko
A
D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D008271:Mycetoma / Q000382:microbiology* / Q000473:pathology; D009615:Nocardia / Q000235:genetics / Q000302:isolation & purification*; D009617:Nocardia Infections / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S