Hypoxic tolerance induction in rat brain slices following hypoxic preconditioning due to expression of neuroprotective proteins as revealed by dynamic changes in glucose metabolism.
We prepared rat brain slices following sublethal hypoxic pretreatment (preconditioning) and untreated (control) rats, and measured the cerebral glucose metabolic rate (CMRglc) by dynamic positron autoradiography with [18F]2-fluoro-2-deoxy-D-glucose before and after originally lethal 20-min hypoxic loading. In the regions of interest such as the frontal cortex, the CMRglc before hypoxic loading did not differ between the preconditioning and control groups. The CMRglc after reoxygenation was markedly lower than that before hypoxic loading in the control group but did not significantly differ from the preloading value in the preconditioning group. Thus, hypoxic tolerance induction by preconditioning was demonstrated using the maintenance of CMRglc as a neuronal viability index. In addition, profiling of gene expression using an Atlas Rat Stress Array suggested the involvement of the expression of genes such as stress protein in hypoxic tolerance induction.
Omata
Naoto
N
Department of Neuropsychiatry, Fukui Medical University, Fukui 910-1193, Japan.
Murata
Tetsuhito
T
Takamatsu
Shinji
S
Maruoka
Nobuyuki
N
Wada
Yuji
Y
Yonekura
Yoshiharu
Y
Fujibayashi
Yasuhisa
Y
D000818:Animals; D001921:Brain / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D015687:Cell Hypoxia / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D005947:Glucose / Q000096:biosynthesis*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D002534:Hypoxia, Brain / Q000378:metabolism / Q000503:physiopathology*; D066298:In Vitro Techniques; D019194:Ischemic Preconditioning / Q000379:methods / Q000706:statistics & numerical data; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D006360:Heat-Shock Proteins; D005947:Glucose