title: Enhancement of circulating dendritic cell activity by immunomodulators (OK432 and KP-40).
abstract: The effects of biological response modifiers (BRMs) on the functions of two types of dendritic cells (DCs) were examined in relation to anti-tumor therapy. The two BRMs studied in our assay system were OK432 (a streptococcal preparation) and KP-40 (Propionibacterium avidum: a heat-inactivated bacterial vaccine). Recently, techniques for isolating human DCs from peripheral blood have been established, and DCs can be divided into two subsets: CD11c+ DCs (myeloid DC population) and CD11c- DCs (lymphoid DC population). Both OK432 and KP-40 were found to up-regulate the activity of myeloid-lineage DCs: the expression of major histocompatibility complex (MHC) class II molecules and adhesion/costimulatory molecules increased, and the production of IL-12 also increased. Therefore, DCs pulsed with OK432 and KP-40 could be applied to vaccination as a new adjuvant for specific immunotherapies.
authors.affiliation: First Department of Surgery, Kansai Medical University, Moriguchi, Osaka, Japan.
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Toyokawa
authors.forename: Muneo
authors.initials: M
authors.lastname: Inaba
authors.forename: Soichiro
authors.initials: S
authors.lastname: Takai
authors.forename: Sohei
authors.initials: S
authors.lastname: Satoi
authors.forename: Josef
authors.initials: J
authors.lastname: Beuth
authors.forename: H L
authors.initials: HL
authors.lastname: Ko
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Matsui
authors.forename: A-Hon
authors.initials: AH
authors.lastname: Kwon
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kamiyama
authors.forename: Susumu
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D001428:Bacterial Vaccines; D002478:Cells, Cultured; D003712:Dendrites / Q000187:drug effects / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D010844:Picibanil / Q000494:pharmacology*; D015164:Vaccines, Inactivated / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001428:Bacterial Vaccines; C465012:KP 40 vaccine; D015164:Vaccines, Inactivated; D010844:Picibanil