Fine needle aspiration of Langerhans cell histiocytosis of the lymph nodes. A report of six cases.
BACKGROUND
Fine needle aspiration (FNA) diagnosis of Langerhans cell histiocytosis (LCH) of the lymph nodes has been described rarely.


METHODS
LCH was confined to the lymph nodes in six children. The FNA smears showed high cellularity composed of many isolated Langerhans cells (LCs) with nuclear grooves and intranuclear inclusions. Also seen were numerous eosinophils, lymphocytes, giant cells, dendriticlike cells, macrophages and Charcot-Leyden crystals in a background of eosinophilic granules. Immunohistochemical study revealed a positive reaction with S-100 protein.


CONCLUSIONS
The presence of LCs with nuclear grooves, eosinophils, giant cells and a positive reaction with S-100 protein aided the diagnosis of LCH of the lymph nodes. Charcot-Leyden crystals, intranuclear inclusions and dendriticlike cells were seen in this study. These findings have not been reported before in lymph node FNA smears of LCH.
Kumar
Perikala V
PV
Department of Pathology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
Mousavi
Abdullah
A
Karimi
Mehran
M
Bedayat
Gholam Reza
GR
D001707:Biopsy, Needle; D002452:Cell Count; D002467:Cell Nucleus / Q000648:ultrastructure; D002648:Child; D004804:Eosinophils / Q000473:pathology*; D006023:Glycoproteins / Q000032:analysis; D006644:Histiocytes / Q000473:pathology*; D006646:Histiocytosis, Langerhans-Cell / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000473:pathology*; D008245:Lysophospholipase; D011110:Polymorphism, Genetic; D009418:S100 Proteins / Q000032:analysis / Q000276:immunology
D006023:Glycoproteins; D009418:S100 Proteins; D008245:Lysophospholipase; C011711:lysolecithin acylhydrolase