Identification of a single genome by electron paramagnetic resonance (EPR) with nitroxide-labeled oligonucleotide probes.
Nitroxide-labeled nucleic acids are used as a molecular size sensor to identify as few as one genome under polymerase chain reaction (PCR) conditions by electron paramagnetic resonance (EPR) spectroscopy. DNA identification is based on differences in the EPR spectra of mononitroxide-labeled nucleic acids. The experimental data imply that rapid DNA identification can be achieved in many systems by EPR at the molecular level.
Hester
Jeff D
JD
Department of Chemistry, University of Cincinnati, OH 45221-0172, USA.
Bobst
Elizabeth V
EV
Kryak
David D
DD
Bobst
Albert M
AM
D000818:Animals; D002942:Circular Dichroism; D003497:Cyclic N-Oxides / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000032:analysis*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D016819:Encephalitozoon / Q000737:chemistry / Q000235:genetics*; D018503:Genome, Protozoan; D009589:Nitrogen Oxides / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet; D013113:Spin Labels
D003497:Cyclic N-Oxides; D017931:DNA Primers; D016054:DNA, Protozoan; D009589:Nitrogen Oxides; D015345:Oligonucleotide Probes; D013113:Spin Labels; C039900:nitroxyl