Differentially regulated, vegetative-mycelium-specific hydrophobins of the edible basidiomycete Pleurotus ostreatus.
Three different hydrophobins (Vmh1, Vmh2, and Vmh3) were isolated from monokaryotic and dikaryotic vegetative cultures of the edible fungus Pleurotus ostreatus. Their corresponding genes have a number of introns different from those of other P. ostreatus hydrophobins previously described. Two genes (vmh1 and vmh2) were expressed only at the vegetative stage, whereas vmh3 expression was also found in the fruit bodies. Furthermore, the expression of the three hydrophobins varied significantly with culture time and nutritional conditions. The three genes were mapped in the genomic linkage map of P. ostreatus, and evidence is presented for the allelic nature of vmh2 and POH3 and for the different locations of the genes coding for the glycosylated hydrophobins Vmh3 and POH2. The glycosylated nature of Vmh3 and its expression during vegetative growth and in fruit bodies suggest that it should play a role in development similar to that proposed for SC3 in Schizophyllum commune.
Peñas
María M
MM
Departamento de Producción Agraria, Universidad Pública de Navarra, E-31006 Pamplona, Spain.
Rust
Brian
B
Larraya
Luis M
LM
Ramírez
Lucía
L
Pisabarro
Antonio G
AG
D000483:Alleles; D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D003470:Culture Media; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D020076:Pleurotus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
D003470:Culture Media; D005656:Fungal Proteins