Dopamine transmission in DYT1 dystonia: a biochemical and autoradiographical study.
Indices of dopamine transmission were measured in the postmortem striatum of DYT1 dystonia brains. A significant increase in the striatal 3,4-dihydroxyphenylacetic acid/dopamine ratio was found. Quantitative autoradiography revealed no differences in the density of dopamine transporter or vesicular monoamine transporter-2 binding; however, there was a trend toward a reduction in D(1) receptor and D(2) receptor binding. One brain with DYT1 parkinsonism was similarly evaluated and marked reductions in striatal dopamine, 3,4-dihydroxyphenylacetic acid, and homovanillic acid content as well as the density of binding of all four dopaminergic ligands were measured.
Augood
Sarah J
SJ
Center for Aging, Genetics, and Neurodegeneration, Massachusetts General Hospital and Harvard Medical School, 114 16th Street, Charlestown, MA 02129, USA. augood@helix.mgh.harvard.edu
Hollingsworth
Z
Z
Albers
D S
DS
Yang
L
L
Leung
J-C
JC
Muller
B
B
Klein
C
C
Breakefield
X O
XO
Standaert
D G
DG
D001345:Autoradiography; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D003342:Corpus Striatum / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D004298:Dopamine / Q000032:analysis / Q000378:metabolism*; D050483:Dopamine Plasma Membrane Transport Proteins; D004422:Dystonia Musculorum Deformans / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000032:analysis / Q000378:metabolism; D008875:Middle Aged; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D011954:Receptors, Dopamine / Q000032:analysis / Q000378:metabolism; D050492:Vesicular Biogenic Amine Transport Proteins; D050493:Vesicular Monoamine Transport Proteins
D002352:Carrier Proteins; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D011954:Receptors, Dopamine; C108175:TOR1A protein, human; D050492:Vesicular Biogenic Amine Transport Proteins; D050493:Vesicular Monoamine Transport Proteins; D004298:Dopamine