Multilaboratory comparison of anaerobe susceptibility results using 3 different agar media.
A 5-laboratory study was performed that used the National Committee for Clinical Laboratory Standards (NCCLS) reference agar dilution method with 3 media formulations to determine whether the use of different media would affect minimum inhibitory concentration (MIC) results. Wilkins-Chalgren, Brucella-based blood agar (BRU), and Wilkins-Chalgren agar plus blood (WCB) and 6 antibiotics (clindamycin, cefoxitin, ceftizoxime, piperacillin, metronidazole, and trovafloxacin) were evaluated with 58 isolates. The MIC values were compared, and a significant correlation of >0.80 was demonstrated for all media and each antibiotic/organism group. The cumulative rate of errors for all antibiotics was 0.1%. These data indicate that a change in the NCCLS reference medium for testing of anaerobic bacteria susceptibility to either BRU or WCB will not affect the MIC results for the antibiotics and organisms evaluated.
Roe
D E
DE
Anaerobe Systems, Morgan Hill, CA, USA.
Finegold
S M
SM
Citron
D M
DM
Goldstein
E J C
EJ
Wexler
H M
HM
Rosenblatt
J E
JE
Cox
M E
ME
Jenkins
S G
SG
Hecht
D W
DW
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000302:isolation & purification; D001769:Blood; D003470:Culture Media; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010837:Vitamin K 1 / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D003470:Culture Media; D006427:Hemin; D010837:Vitamin K 1