Estrogen receptor specificity for the effects of estrogen in ovariectomized mice.
Estrogen exerts a variety of important physiological effects, which have been suggested to be mediated via the two known estrogen receptors (ERs), alpha and beta. Three-month-old ovariectomized mice, lacking one or both of the two estrogen receptors, were given estrogen subcutaneously (2.3 micro g/mouse per day) and the effects on different estrogen-responsive parameters, including skeletal effects, were studied. We found that estrogen increased the cortical bone dimensions in both wild-type (WT) and double ER knockout (DERKO) mice. DNA microarray analysis was performed to characterize this effect on cortical bone and it identified four genes that were regulated by estrogen in both WT and DERKO mice. The effect of estrogen on cortical bone in DERKO mice might either be due to remaining ERalpha activity or represent an ERalpha/ERbeta-independent effect. Other effects of estrogen, such as increased trabecular bone mineral density, thymic atrophy, fat reduction and increased uterine weight, were mainly ERalpha mediated.
Lindberg
M K
MK
Division of Endocrinology, Department of Internal Medicine, Gröna Stråket 8, Sahlgrenska University Hospital, S-41345 Göteborg, Sweden.
Weihua
Z
Z
Andersson
N
N
Movérare
S
S
Gao
H
H
Vidal
O
O
Erlandsson
M
M
Windahl
S
S
Andersson
G
G
Lubahn
D B
DB
Carlsten
H
H
Dahlman-Wright
K
K
Gustafsson
J-A
JA
Ohlsson
C
C
D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects*; D024102:Collagen Type VIII / Q000235:genetics; D003176:Complement C3 / Q000235:genetics; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D058574:Integrin-Binding Sialoprotein; D007377:Interleukin-3 / Q000235:genetics; D008099:Liver / Q000187:drug effects; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D009929:Organ Size / Q000187:drug effects; D010052:Ovariectomy; D012333:RNA, Messenger / Q000032:analysis; D011960:Receptors, Estrogen / Q000235:genetics / Q000502:physiology*; D012795:Sialoglycoproteins / Q000235:genetics; D013950:Thymus Gland / Q000166:cytology; D014599:Uterus / Q000166:cytology / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000235:genetics
D024102:Collagen Type VIII; D003176:Complement C3; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; C546872:Ibsp protein, mouse; D058574:Integrin-Binding Sialoprotein; D007377:Interleukin-3; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D012795:Sialoglycoproteins; D000511:alpha-Macroglobulins; D020780:Matrix Metalloproteinase 9