title: Molecular cloning and characterization of plant genes encoding novel peroxisomal molybdoenzymes of the sulphite oxidase family.
abstract: The Arabidopsis AtMCP and rice OsMCP genes which encode proteins highly homologous to molybdoenzymes of the sulphite oxidase family were isolated and characterized. Both proteins seemed to possess only a molybdenum cofactor as the redox centre, unlike all the other eukaryotic molybdoenzymes. Putative MCP orthologues were identified in 17 plant species, indicating that MCPs are widely distributed over the plant kingdom [corrected]. An analysis using a green fluorescent protein fusion showed that AtMCP possesses a peroxisomal targeting signal at its C-terminus. Putative peroxisomal targeting signals were also found in all plant MCPs, suggesting the existence of a new redox pathway in this organelle.
authors.affiliation: Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630-0101, Japan. t-nakamu@ynu.ac.jp
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Christian
authors.initials: C
authors.lastname: Meyer
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sano
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D008982:Molybdenum / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000235:genetics* / Q000378:metabolism; D020675:Peroxisomes / Q000201:enzymology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D008164:Luminescent Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D008982:Molybdenum; D050862:Oxidoreductases Acting on Sulfur Group Donors