Cloning of Schistosoma japonicum Chinese Strain Fatty Acid Binding Protein (Sj-FABPc) Gene and Its Overproduction in Escherichia coli.
A 600 bp DNA fragment was amplified by PCR, from an adult Schistosoma japonicum cDNA library. Sequence analysis revealed that this fragment containedthe S. japonicum Chinese Mainland strain fatty acid binding protein (Sj-14FABPc) gene. This gene was then cloned into the expression vector pGEX-2T, and subsequently expressed in Escherichia coli. The recombinant GST-fusion protein could be purified by glutathione agarose affinity chromatography. Its molecular weight was about 41 kD. The yield of expression was around 25 mg/L E. coli culture. The immunological test suggested that the recombinant protein had good antigenicity, and could be developed into a new vaccine molecule of S. japonicum.
Cai
Xue-Zhong
XZ
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
Tian
E
E
Yang
Guan-Zhen
GZ
Shi
Fu-Hui
FH
Shen
Wei
W
Cai
You-Min
YM
Wu
Xiang-Fu
XF