Effects of luteolin on the inhibition of proliferation and induction of apoptosis in human myeloid leukaemia cells.
Luteolin, a flavonoid isolated from the fruit of Vitex rotundifolia, has been examined with regard to the inhibition of proliferation and induction of apoptosis in human myeloid leukaemia HL-60 cells. The concentration required for 50% inhibition of the growth after 96 h was 15 +/- 1.1 microM. The mode of cell death induced by luteolin was found to be apoptosis, as judged by the morphologic alteration of the cells and by the detection of DNA fragmentation using agarose gel electrophoresis. The degree of apoptosis was quantified by a sandwich enzyme immunoassay and flow cytometric analysis. These results suggest that luteolin may be used as potential chemopreventive and chemotherapeutic agents.
Ko
W G
WG
College of Pharmacy and Medicinal Resources Research Center, Wonkwang University, Iksan, Jeonbuk, Korea.
Kang
T H
TH
Lee
S J
SJ
Kim
Y C
YC
Lee
B H
BH
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005434:Flow Cytometry; D005638:Fruit / Q000737:chemistry; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D007951:Leukemia, Myeloid / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D047311:Luteolin; D015394:Molecular Structure; D020950:Vitex
D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D047311:Luteolin