title: Ion channels in secretory granules of the pancreas and their role in exocytosis and release of secretory proteins.
abstract: Regulated secretion in exocrine and neuroendocrine cells occurs through exocytosis of secretory granules and the subsequent release of stored small molecules and proteins. The introduction of biophysical techniques with high temporal and spatial resolution, and the identification of Ca(2+)-dependent and -independent "docking" and "fusion" proteins, has greatly enhanced our understanding of exocytosis. The cloning of families of ion channel proteins, including intracellular ion channels, has also revived interest in the role of secretory granule ion channels in exocytotic secretion. Thus secretory granules of pancreatic acinar cell express a ClC-2 Cl(-) channel, a HCO-permeable member of the CLCA Ca(2+)-dependent anion channel family, and a KCNQ1 K(+) channel. Evidence suggests that these channels may facilitate the release of digestive enzymes and/or prevent exocytosed granules from collapsing during "kiss and run" recycling. In pancreatic beta-cells, a granular ClC-3 Cl(-) channel provides a shunt pathway for a vacuolar-type H(+)-ATPase. Acidification "primes" the granules for Ca(2+)-dependent exocytosis and release of insulin. In summary, secretory granules are equipped with specific sets of ion channels, which modulate regulated exocytosis and the release of macromolecules. These channels could represent excellent targets for therapeutic interventions to control exocytotic secretion in relevant diseases, such as pancreatitis, cystic fibrosis, or diabetes mellitus.
authors.affiliation: School of Biological Sciences, University of Manchester, United Kingdom. frank.thevenod@man.ac.uk
authors.forename: Frank
authors.initials: F
authors.lastname: Thévenod
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000818:Animals; D018118:Chloride Channels / Q000502:physiology; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007473:Ion Channels / Q000502:physiology*; D051656:KCNQ Potassium Channels; D051657:KCNQ1 Potassium Channel; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism / Q000502:physiology; D024661:Potassium Channels, Inwardly Rectifying; D024642:Potassium Channels, Voltage-Gated; D011506:Proteins / Q000378:metabolism*; D011955:Receptors, Drug / Q000378:metabolism; D022142:Secretory Vesicles / Q000378:metabolism*; D064233:Sulfonylurea Receptors
chemical_list: D018528:ATP-Binding Cassette Transporters; D018118:Chloride Channels; D007473:Ion Channels; D051656:KCNQ Potassium Channels; D051657:KCNQ1 Potassium Channel; C496909:KCNQ1 protein, human; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D024642:Potassium Channels, Voltage-Gated; D011506:Proteins; D011955:Receptors, Drug; D064233:Sulfonylurea Receptors