Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and proteomics: a new era in anaerobic microbiology.
Genome sequence data provide a framework for predicting potential microbial activities; however, the proteome content of the cell dictates its response to its environment. Microbiology is witnessing a major initiative to elucidate the nature of the proteome of large numbers of species. The tool driving the proteomic revolution is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. During the analysis process, proteins are ionized and separated on the basis of their mass-to-charge ratios, which results in a characteristic mass-spectral profile. Because of the dynamic nature of the cell and the large number of external parameters that could influence its mass-spectral profile, considerable work was needed initially to optimize sample analysis and obtain consistent and reproducible results. For many anaerobes that grow poorly or are nonreactive in most diagnostic systems, proteome analysis is likely to have a major impact on microbial diagnosis and the delineation of centers of diversity associated with infections.
Shah
Haroun N
HN
Molecular Identification Services Unit, National Collection of Type Cultures, and Genomics, Proteomics, and Bioinformatics Unit, PHLS Central Public Health Laboratory, London, England. hshah@phls.org.uk
Keys
Carrina J
CJ
Schmid
Oliver
O
Gharbia
Saheer E
SE
D001421:Bacteria, Anaerobic / Q000737:chemistry* / Q000378:metabolism; D016967:Fusobacterium nucleatum / Q000737:chemistry; D017861:Porphyromonas / Q000737:chemistry; D018713:Prevotella intermedia / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D020543:Proteome