Retinoblastoma-related gene RB2/p130 exons 19-22 are rarely mutated in glioblastomas.
The retinoblastoma gene family RB1, p107 and RB2/p130 cooperate to regulate cell cycle progression through the G1 phase of the cell cycle. Previous data demonstrated that RB2/p130 inhibits proliferation of the glioblastoma cell line T98G, which is resistant to the growth suppressive effects of both RB1 and p107, and that RB2/p130 gene overexpresion induces astrocyte differentiation. We screened by single-strand conformation polymorphism and sequence analysis the structure of exons 19 through 22 of the RB2/p130 gene, which encodes the B domain and C terminus, in a series of 42 glioblastomas (32 primary and 10 secondary). Sequence variations were identified in one tumor, suggesting that mutation inactivation of RB2/p130 is a rare event in glioblastoma.
Gonzalez-Gomez
Pilar
P
Departamento C. Experimental, Hospital Universitario La Paz, Paseo de la Castellana 261, 28046 Madrid, Spain.
Bello
M Josefa
MJ
Arjona
Dolores
D
Alonso
M Eva
ME
Lomas
Jesus
J
De Campos
Jose M
JM
Vaquero
Jesus
J
Isla
Alberto
A
Gutierrez
Manuel
M
Rey
Juan A
JA
D001253:Astrocytes / Q000166:cytology; D001483:Base Sequence; D002453:Cell Cycle; D002454:Cell Differentiation; D002455:Cell Division; D004252:DNA Mutational Analysis; D005091:Exons; D016193:G1 Phase; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D010750:Phosphoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017434:Protein Structure, Tertiary; D011506:Proteins; D050717:Retinoblastoma-Like Protein p130; D014407:Tumor Cells, Cultured
D010750:Phosphoproteins; D011506:Proteins; C493837:RBL2 protein, human; D050717:Retinoblastoma-Like Protein p130