title: Brucella sp. bind to sialic acid residues on human and animal red blood cells.
abstract: We report that Brucella abortus and Brucella melitensis agglutinate human (A+ and B+), hamster and rabbit erythrocytes, a heretofore undescribed feature in this genus. This activity was associated with a 29-kDa surface protein (SP29) that bound selectively to these erythrocytes and this binding was inhibited by rabbit anti-SP29 antibodies. Hemagglutination was inhibited by pretreatment of erythrocytes with neuraminidase and by preincubation of B. abortus with chondroitin sulfate, N-acetylneuraminic acid and N-acetylneuramin-lactose.
authors.affiliation: Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio 76, Complejo de Ciencias, Cd. Universitaria, Pue. C.P. 72000, Puebla, Mexico
authors.forename: Rosa
authors.initials: R
authors.lastname: del C  Rocha-Gracia
authors.forename: Elsa I
authors.initials: EI
authors.lastname: Castañeda-Roldán
authors.forename: Silvia
authors.initials: S
authors.lastname: Giono-Cerezo
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Girón
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000937:Antigen-Antibody Reactions; D001422:Bacterial Adhesion; D002002:Brucella / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology; D002809:Chondroitin Sulfates / Q000494:pharmacology; D006224:Cricetinae; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D006386:Hemagglutination Tests; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009439:Neuraminidase / Q000494:pharmacology; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D011956:Receptors, Cell Surface; C092488:sialic acid receptor; D002809:Chondroitin Sulfates; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid