title: Comparison of Giemsa and acridine orange stains for the diagnosis of Leishmania donovani in biopsies of infected hamsters.
abstract: Identical impression smears of spleen, liver and bone marrow biopsy materials from Leishmania donovani-infected hamsters were stained using either acridine orange or Giemsa. Spleen parasite-loads calculated from the two stains for identical biopsy material were significantly different from each other. However, liver and bone marrow parasite- loads calculated from either Giemsa-stained or acridine orange-stained biopsies were not significantly different from each other. This study has shown that acridine orange, which is a quick and simple technique, has great potential in the diagnosis of kala-azar when liver and bone marrow biopsies are used.
authors.affiliation: Kenya Medial Research Institute, Biomedical Sciences Research Centre, P.O. Box 54840, Nairobi, Kenya.
authors.forename: Peter A.
authors.initials: PA
authors.lastname: Mbati
authors.forename: Kisia
authors.initials: K
authors.lastname: Abok
authors.forename: Christopher O.
authors.initials: CO
authors.lastname: Anjili
authors.forename: Alloys/FIRST_NAME> S. Orago Jim S.
authors.initials: AS
authors.lastname: Orago
authors.forename: Jim M.
authors.initials: JM
authors.lastname: Kagai
authors.forename: John I.
authors.initials: JI
authors.lastname: Githure
authors.forename: Davy K.
authors.initials: DK
authors.lastname: Koech