Image reconstruction through turbid media under a transmission-mode microscope.
In this paper, image enhancement and reconstruction through a turbid medium by utilizing polarization gating and mathematical image reconstruction methods in a microscopic imaging system are investigated. A Monte Carlo simulation model based on Mie theory and the concept of the effective point spread function (EPSF) is adopted to study image formation under a transmission-mode microscope. The results show that polarization gating methods, and particularly the differential polarization gating method, can be efficient in suppressing highly scattered light, which leads to a significant enhancement of image quality. An image reconstruction method based on the concept of the EPSF is demonstrated to be efficient in further improving image quality.
Gan
Xiaosong
X
Swinburne University of Technology, Centre for Micro-Photonics, School of Biophysical Sciences and Electrical Engineering, P.O. Box 218 Hawthorn 3122, Victoria, Australia. xgan@swin.edu.au
Gu
Min
M
D055592:Biophysical Phenomena; D001703:Biophysics; D007091:Image Processing, Computer-Assisted / Q000379:methods* / Q000706:statistics & numerical data; D008853:Microscopy; D008962:Models, Theoretical; D009010:Monte Carlo Method; D009391:Nephelometry and Turbidimetry; D055095:Optics and Photonics; D012542:Scattering, Radiation