title: Effects of high pressure on glucose transport in the human erythrocyte.
abstract: The effects of raised hydraulic pressure on D-glucose exit from human red cells at 25 degrees C were determined using light scattering measurements in a sealed pressurized spectrofluorimeter cuvette. The reduction in the rates of glucose exit with raised pressure provides an index of the activation volume, deltaV++ (delta ln k/deltaP)(T) = -deltaV++/RT. Raised pressure decreased the rate constant of glucose exit from 0.077 +/- 0.003 s(-1) to 0.050 +/- 0.002 s(-1) (n = 5, P < 0.003). The Ki for glucose binding to the external site was 2.7 +/- 0.4 mm (0.1 MPa) and was reduced to 1.45 +/- 0.15 mm (40 MPa), (P < 0.01, Student's t test). Maltose had a biphasic effect on deltaV++. At [maltose] <250 microM, deltaV++ of glucose exit increased above that with [maltose = 0 mM], at >1 mm maltose, deltaV++ was reduced below that with [maltose = 0 mM]. Pentobarbital (2 mM) decreased the deltaV++ of net glucose exit into glucose-free solution from 30 +/- 5 ml mol(-1) (control) to 2 +/- 0.5 ml mol(-1) (P < 0.01). Raised pressure had a negligible effect on L-sorbose exit. These findings suggest that stable hydrated and liganded forms of GLUT with lower affinity towards glucose permit higher glucose mobilities across the transporter and are modelled equally well with one-alternating or a two-fixed-site kinetic models.
authors.affiliation: Research Center for Cardiovascular Biology and Medicine, GKT School of Biomedical Sciences, King's College London, United Kingdom. richard.naftalin@kcl.ac.uk
authors.forename: R J
authors.initials: RJ
authors.lastname: Naftalin
authors.forename: I
authors.initials: I
authors.lastname: Afzal
authors.forename: J A
authors.initials: JA
authors.lastname: Browning
authors.forename: R J
authors.initials: RJ
authors.lastname: Wilkins
authors.forename: J C
authors.initials: JC
authors.lastname: Ellory
mesh_terms: D001692:Biological Transport; D001786:Blood Glucose / Q000378:metabolism*; D003198:Computer Simulation; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D051272:Glucose Transporter Type 1; D006801:Humans; D066298:In Vitro Techniques; D008954:Models, Biological; D008956:Models, Chemical; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D010424:Pentobarbital / Q000008:administration & dosage; D011312:Pressure; D012680:Sensitivity and Specificity; D013013:Sorbose / Q000378:metabolism; D014867:Water / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; C495677:SLC2A1 protein, human; D014867:Water; D010424:Pentobarbital; D013013:Sorbose