Ultrastructural localization of orexin-1 receptor in pre- and post-synaptic neurons in the rat arcuate nucleus.
The distribution and ultrastructural localization of the orexin-1 receptor (OX(1)R) in the rat arcuate nucleus were studied using immunocytochemical techniques. OX(1)R-containing neurons were found throughout the nucleus, but were concentrated in its posterior region. Both OX(1)R-positive perikarya and dendrites were found to receive synapses from other unknown axon terminals. In addition, a small number of OX(1)R-positive axon terminals were observed. These OX(1)R-immunoreactive axon terminals were often found to make synapses on unidentified immunonegative dendritic processes. The present results indicate that orexin may act on food intake regulating neurons through both pre- and post-synaptic OX(1)R.
Guan
Jian-Lian
JL
Department of Anatomy, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Suzuki
Ryusuke
R
Funahashi
Hisayuki
H
Wang
Qing-Ping
QP
Kageyama
Haruaki
H
Uehara
Kichio
K
Yamada
Shuori
S
Tsurugano
Shinobu
S
Shioda
Seiji
S
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000737:chemistry / Q000648:ultrastructure*; D008297:Male; D009474:Neurons / Q000737:chemistry / Q000648:ultrastructure*; D064446:Orexin Receptors; D017729:Presynaptic Terminals / Q000737:chemistry / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide / Q000032:analysis / Q000648:ultrastructure*
D064446:Orexin Receptors; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide