Early inflammatory markers in elicitation of allergic contact dermatitis.
BACKGROUND
Allergic Contact Dermatitis (ACD) is regarded as a T-cell-mediated delayed-type hypersensitivity reaction. We studied the kinetics of the expression of CS-1 fibronectin, thymus and activation-regulated chemokine (CCL17/ TARC) and different chemokine receptors (CR) in skin biopsies from individuals suffering from back problems, with the antigen responsible of their contact dermatitis and an irrelevant antigen.


METHODS
Samples were taken at 2, 10, and 48 hours for histological and immunohistochemical studies using monoclonal antibodies against human CS-1 fibronectin, CCL17, CD3, CD68, CD49d, CXCR3, CCR5, and CCR3.


RESULTS
At positive antigen stimulated sites there was an early expression of CS-1 fibronectin (2 hours), followed by CCL17 and a later accumulation of alplha4/beta1+ (CD49d), CD3+, CD68+, CXCR3+ and CCR5+ mononuclear cells. At 48 hours, approximately 59 % of infiltrating cells were CXCR3+, 42% CCR5+, and only 14 % CCR3+.


CONCLUSIONS
These results showed for the first time a very early expression of CS-1 fibronectin which preceded production of CCL17 in blood endothelial cells (BCEs) from patients' skin with ACD. The role of these molecules in recruitment of monocytes and effector T cells in ACD is discussed.
Martín
Andrea
A
Dpto Bioquímica Clínica, Facultad de Ciencias Químicas-Universidad Nacional de Córdoba, Córdoba, Argentina. amartin@bioclin.fcq.unc.edu.ar
Gallino
Norberto
N
Gagliardi
Julio
J
Ortiz
Susana
S
Lascano
Alejandro Ruiz
AR
Diller
Ana
A
Daraio
María Cristina
MC
Kahn
Adrián
A
Mariani
Ana Lía
AL
Serra
Horacio Marcelo
HM
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D017252:CD3 Complex / Q000032:analysis; D054414:Chemokine CCL17; D019742:Chemokines, CC / Q000032:analysis; D017449:Dermatitis, Allergic Contact / Q000473:pathology*; D005353:Fibronectins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D039441:Integrin alpha4 / Q000032:analysis; D015603:Keratinocytes / Q000737:chemistry; D008875:Middle Aged; D019713:Receptors, CCR5 / Q000032:analysis; D054399:Receptors, CCR6; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000032:analysis*; D012867:Skin / Q000737:chemistry* / Q000473:pathology*; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D017252:CD3 Complex; D054414:Chemokine CCL17; D019742:Chemokines, CC; D005353:Fibronectins; D039441:Integrin alpha4; D019713:Receptors, CCR5; D054399:Receptors, CCR6; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine; C106230:CCL17 protein, human; C106311:CCR6 protein, human; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C515187:CXCR3 protein, human