title: Development of an optimized, simple chemically defined medium for bacterial cellulose production by Acetobacter sp. A9 in shaking cultures.
abstract: The genus Acetobacter can synthesize cellulose when grown in an undefined medium containing glucose. By using the technique of the omission of a single medium component, an optimized and simple chemically defined medium was developed to support cellulose production by Acetobacter sp. A9 in shaking culture. It contained 4.0% (w/v) glucose, 0.2% (w/v) (NH(4))(2)SO(4), 0.25% (w/v) KH(2)PO(4), 0.3% (w/v) Na(2)HPO(4).12H(2)O, 0.05% (w/v) MgSO(4).7H(2)O, 0.0002% (w/v) FeSO(4).7H(2)O, 0.00025% (w/v) H(3)BO(3), 0.00006% (w/v) nicotinamide, 0.00025% (w/v) inositol and 1.4% (v/v) ethanol. A maximum cellulose concentration of around 8 g/l was achieved after 9 days of cultivation at 200 rev./min. The production of cellulose by Acetobacter sp. A9 was greater in simplified synthetic medium than in complex medium (Hestrin and Schramm medium) conventionally used for Acetobacter strains.
authors.affiliation: Faculty of Applied Marine Science, Cheju National University, Jeju 690-756, South Korea.
authors.forename: Moon-Soo
authors.initials: MS
authors.lastname: Heo
authors.forename: Hong-Joo
authors.initials: HJ
authors.lastname: Son
mesh_terms: D000091:Acetobacter / Q000145:classification / Q000378:metabolism*; D002482:Cellulose / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011786:Quality Control; D012680:Sensitivity and Specificity
chemical_list: D003470:Culture Media; D011135:Polysaccharides, Bacterial; D000431:Ethanol; D002482:Cellulose; D005947:Glucose