Isolation and partial characterisation of a protein from buck seminal plasma (Capra hircus), homologous to spermadhesins.
Spermadhesins are a family of secretory proteins expressed in the male genital tract of pig, horse and bull. Their function and structure have been widely studied, especially those isolated from boar. However, there are no data concerning spermadhesins isolated from buck. Buck seminal plasma was collected and subjected to ion exchange chromatography on DEAE-Sephacel column followed by chromatography in a C18 column coupled to a HPLC system. The purification of the protein was determined by SDS-PAGE and MALDI-TOF analysis exhibiting a molecular mass of 12.5 KDa and showed to be structurally homologous to spermadhesins from boar and stallion.
Teixeira
D I A
DI
State University of Ceará, Laboratory of Physiology and Control of Reproduction, Fortaleza, Brazil CE 60740-000.
Cavada
B S
BS
Sampaio
A H
AH
Havt
A
A
Bloch
C
C
Prates
M V
MV
Moreno
F B M B
FB
Santos
E A
EA
Gadelha
C A A
CA
Gadelha
T S
TS
Crisóstomo
F S M
FS
Freitas
V J F
VJ
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D006041:Goats; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D029589:Seminal Plasma Proteins; C425042:spermadhesin