title: Comparing transforming growth factor-beta2, talc and bleomycin as pleurodesing agents in sheep.
abstract: BACKGROUND
  Transforming growth factor (TGF)-beta2 can produce effective pleurodesis in animals, but its efficacy has not been compared with commonly used pleurodesing agents in sheep, which have a thick pleura resembling that of humans. The acute physiological effects and the level of systemic TGF-beta absorption after its intrapleural administration have not been studied. The aims of the present study were to compare: (i) the effectiveness of TGF-beta2, talc and bleomycin in producing pleurodesis in sheep; (ii) the acute side-effects and systemic TGF-beta levels following the intrapleural administration of these agents; and (iii) histological changes after intrapleural injections of these agents.
  
  
  METHODS
  Twelve sheep were divided into three groups and were given a single intrapleural dose of TGF-beta2 (0.25 microg/kg), talc slurry (5 g) or bleomycin (60 IU) via a chest tube. Saline or buffer was injected into the contralateral side, which served as the control. Arterial blood gases and respiratory and heart rates were monitored for the first 24 h. Plasma levels of TGF-beta1 and TGF-beta2 were measured. Pleurodesis was graded macroscopically from 1 (none) to 8 (symphysis > 50% of hemithorax) at day 14.
  
  
  RESULTS
  At day 14, the pleurodesis score of the TGF-beta2 group (7.7+/-0.6) was similar to that of the talc (7.0+/-1.7) group and significantly higher than that of the bleomycin group (3.3+/-2.3; P < 0.05). No significant differences were seen in arterial blood gas analysis, vital signs and plasma TGF-beta1 and TGF-beta2 concentrations among the three groups.
  
  
  CONCLUSIONS
  Transforming growth factor-beta2 was as effective as talc and more so than bleomycin in inducing pleurodesis in sheep. Intrapleural administration of TGF-beta2 appeared safe. No acute changes in gaseous exchange or macroscopic abnormalities were seen following intrapleural TGF-beta2. Importantly, there was no evidence of an increase in systemic TGF-beta levels following its intrapleural administration.
authors.affiliation: Department of Pulmonary Medicine, St Thomas Hospital, Nashville, Tennessee, USA. ycgarylee@hotmail.com
authors.forename: Y C Gary
authors.initials: YC
authors.lastname: Lee
authors.forename: Justin R
authors.initials: JR
authors.lastname: Yasay
authors.forename: Joyce E
authors.initials: JE
authors.lastname: Johnson
authors.forename: Richard E
authors.initials: RE
authors.lastname: Parker
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Thompson
authors.forename: Kirk B
authors.initials: KB
authors.lastname: Lane
authors.forename: Richard W
authors.initials: RW
authors.lastname: Light
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use; D001761:Bleomycin / Q000627:therapeutic use; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D010996:Pleural Effusion / Q000473:pathology / Q000628:therapy*; D018700:Pleurodesis / Q000379:methods*; D012756:Sheep; D018709:Statistics, Nonparametric; D013627:Talc / Q000627:therapeutic use; D016212:Transforming Growth Factor beta / Q000097:blood / Q000627:therapeutic use*; D053781:Transforming Growth Factor beta2
chemical_list: D000903:Antibiotics, Antineoplastic; D007166:Immunosuppressive Agents; D016212:Transforming Growth Factor beta; D053781:Transforming Growth Factor beta2; D001761:Bleomycin; D013627:Talc