Genetically stable picornavirus expression vectors with recombinant internal ribosomal entry sites.
In many respects, picornaviruses are well suited for their proposed use as immunization vectors. However, their inherent genetic instability hinders application for prophylactic purposes. We demonstrate the improved expression and stability of a heterologous insert through a novel vector design strategy that partially replaces noncoding regulatory sequences with coding sequences for foreign gene products.
Dufresne
Andrew T
AT
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Dobrikova
Elena Y
EY
Schmidt
Stacie
S
Gromeier
Matthias
M
D000818:Animals; D001483:Base Sequence; D015195:Drug Design; D004355:Drug Stability; D017353:Gene Deletion; D005822:Genetic Vectors / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D010849:Picornaviridae / Q000235:genetics*; D011995:Recombination, Genetic; D012229:Rhinovirus / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism
D014763:Viral Matrix Proteins