title: Karyological and flow cytometric evidence of triploid specimens in Bufo viridis (Amphibia Anura).
abstract: Karyological and flow cytometric (FCM) analyses were performed on a group of 14 green toads of the Bufo viridis species from seven Eurasian populations. Both approaches gave concordant results concerning the DNA ploidy level. All the populations examined were represented exclusively by diploid or tetraploid specimens, except one, where triploids were found. Results evidenced an interpopulation variability in DNA content against the same ploidy level, as well as an unusually high number of triploids in a particular reproductive place. The origin of polyploidy and the presence and persistence of a high number of triploids in a particular population are discussed.
authors.affiliation: Environmental Department, ENEA C.R. Casaccia, Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Cavallo
authors.forename: R
authors.initials: R
authors.lastname: De Vita
authors.forename: P
authors.initials: P
authors.lastname: Eleuteri
authors.forename: L
authors.initials: L
authors.lastname: Borkin
authors.forename: V
authors.initials: V
authors.lastname: Ermechenko
authors.forename: G
authors.initials: G
authors.lastname: Odiema
authors.forename: E
authors.initials: E
authors.lastname: Balletto
mesh_terms: D000818:Animals; D002025:Bufonidae / Q000235:genetics*; D002467:Cell Nucleus / Q000235:genetics*; D002871:Chromosome Banding; D004247:DNA / Q000032:analysis; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D014644:Genetic Variation; D016678:Genome; D007620:Karyometry; D007621:Karyotyping; D008297:Male; D011123:Polyploidy; D015203:Reproducibility of Results
chemical_list: D004247:DNA