title: Effects of atrial natriuretic peptide on DNA synthesis and NADPH diaphorase activity in epitheliocytes and smooth muscle cells of the respiratory tract in newborn albino rats.
abstract: Newborn rats received single intraperitoneal injections of atrial natriuretic peptide (1-28) in a dose of 3.2x10(-8) mol/kg on day 6 of life. Autoradiography with 3H-thymidine showed that the peptide inhibited DNA synthesis in smooth muscle cells of the respiratory tract. Pretreatment with nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester attenuated, but did not abolish the effect of atrial natriuretic peptide (1-28). Histochemical assay for NADPH diaphorase showed that nitric oxide constitutively produced in the epithelium is involved in the growth-inhibitory effect of atrial natriuretic peptide (1-28) on proliferating smooth muscle cells.
authors.affiliation: Institute of Maternity and Child Welfare, Khabarovsk Branch, Far-Eastern Research Center for Physiology and Pathology of Respiration, Siberian Division of the Russian Academy of Medical Sciences, Far-Eastern State Medical University, Khabarovsk.
authors.forename: O A
authors.initials: OA
authors.lastname: Lebed'ko
authors.forename: S S
authors.initials: SS
authors.lastname: Timoshin
authors.forename: V I
authors.initials: VI
authors.lastname: Tsygankov
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D009320:Atrial Natriuretic Factor / Q000378:metabolism* / Q000494:pharmacology; D001345:Autoradiography; D001980:Bronchi / Q000201:enzymology / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004848:Epithelium / Q000201:enzymology / Q000378:metabolism*; D009130:Muscle, Smooth / Q000201:enzymology / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D051381:Rats; D014132:Trachea / Q000201:enzymology / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D009320:Atrial Natriuretic Factor; D004247:DNA; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase; D019331:NG-Nitroarginine Methyl Ester