Methyl linoleate oxidation in the presence of bovine serum albumin.
The oxidation of methyl linoleate (LMe) in the presence of bovine serum albumin (BSA) was studied to analyze both the processes involved when lipid oxidation occurs in the presence of proteins and the relative progression of the several reactions implicated. The disappearance of LMe, the formation of primary and secondary lipid oxidation products, the loss of essential amino acids, and the production of oxidized lipid/amino acid reaction products (OLAARPs) were studied as a function of incubation time. During the first steps of lipid oxidation, LMe was converted quantitatively to methyl linoleate hydroperoxides, which were very rapidly degraded to either secondary products of lipid oxidation or OLAARPs. No significant differences were identified in the major lipid oxidation products formed in incubations with or without proteins, indicating that mechanisms for formation of these compounds are similar in both cases. In addition, no significant differences were observed between the time-courses of formation of secondary oxidation products and OLAARPs, suggesting that hydroperoxide decomposition and OLAARP formation occur simultaneously when the lipid oxidation process takes place in the presence of proteins. Furthermore, OLAARP formation seems to be an unavoidable process that should be considered as a last step in the lipid peroxidation process.
Instituto de la Grasa, CSIC, Avenida Padre García Tejero 4, 41012 Seville, Spain.
Francisco J
FJ
Hidalgo
Rosario
R
Zamora
D000596:Amino Acids / Q000737:chemistry; D003116:Color; D006868:Hydrolysis; D007700:Kinetics; D008041:Linoleic Acids / Q000737:chemistry*; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000596:Amino Acids; D008041:Linoleic Acids; D017392:Thiobarbituric Acid Reactive Substances; C005575:methyl linoleate; D012710:Serum Albumin, Bovine