Enzyme activity after resealing within ghost erythrocyte cells, and protection by alpha-crystallin against fructose-induced inactivation.
The role of alpha-crystallin as a molecular chaperone has been shown in many in vitro studies. In the present paper, we report on the chaperone function of alpha-crystallin within resealed erythrocyte ghosts. Eight enzymes were individually resealed within erythrocyte ghosts and assayed at zero time and at 24 h. The ghost cell suspension was separated into soluble and membrane fractions. Five of the enzymes had significantly greater enzyme activity after 24 h than the control within the soluble fractions. Fructation caused a decrease in enzyme activity (relative to the control). Resealing of alpha-crystallin within the ghost cell alongside the enzymes protected against inactivation by fructose within the soluble fraction.
Nuffield Laboratory of Ophthalmology, University of Oxford, Walton Street, Oxford OX2 6AW, UK.
Barry K
BK
Derham
John J
JJ
Harding
D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D001665:Binding Sites; D002374:Catalase / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002850:Chromatography, Gel; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000201:enzymology*; D005632:Fructose / Q000378:metabolism*; D005649:Fumarate Hydratase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D050356:Lipid Metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D011487:Protein Conformation; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D038201:alpha-Crystallins / Q000378:metabolism*
D038201:alpha-Crystallins; D005632:Fructose; D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D000444:Aldehyde Dehydrogenase; D005980:Glutathione Reductase; D005649:Fumarate Hydratase