Detection of enterotoxin genes in mosquito-larvicidal Bacillus species.
The presence of the B component of hemolysin BL (hblA), enterotoxin BceT (bceT), and enterotoxin S (entS) genes in mosquito-larvicidal Bacillus sp., including 25 B. sphaericus and 4 B. thuringiensis subsp. israelensis strains, has been analyzed by multiplex PCR in this study. The results showed that all four B. thuringiensis strains contain the hblA gene and the sequences of bceT and entS genes. However, none of the enterotoxin gene sequences were detected in the B. sphaericus strains. The enterotoxin production in all strains has also been analyzed by using two commercial immunoassay kits (TECRA and RPLA), and it has been proved that all the B. thuringiensis strains and one B. cereus strain can produce enterotoxins during growth. No enterotoxin activity could be detected in B. sphaericus strains.
Yuan
Zhiming
Z
Wuhan Institute of Virology, Chinese Academy of Sciences, China. yzm@pentium.whiov.ac.cn
Hansen
Bjarne M
BM
Andrup
Lars
L
Eilenberg
Jørgen
J
D000818:Animals; D001407:Bacillus / Q000235:genetics*; D001409:Bacillus cereus / Q000235:genetics; D001413:Bacillus thuringiensis / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D009033:Culicidae; D004269:DNA, Bacterial / Q000235:genetics; D004731:Endotoxins / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D010572:Pest Control, Biological; D016133:Polymerase Chain Reaction
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004731:Endotoxins; D004768:Enterotoxins; D006460:Hemolysin Proteins; C046134:binB protein, Bacillus sphaericus; C040792:enterotoxin, Bacillus cereus; C083961:hemolysin BL protein, Bacillus; C030956:insecticidal crystal protein, Bacillus Thuringiensis