Simultaneous expression of different variant-specific surface proteins in single Giardia lamblia trophozoites during encystation.
A novel variant-specific surface protein (VSP) from Giardia was identified using the monoclonal antibody 9B10, raised against purified cyst walls. VSP9B10B is preferentially induced during encystation and expressed simultaneously with other VSPs on the surface of encysting trophozoites. These results support the hypothesis that encystation and antigenic variation are processes that are mechanistically related.
Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, CP 5000 Córdoba, Argentina.
Pedro G
PG
Carranza
Gisela
G
Feltes
Andrea
A
Ropolo
Silvia M C
SM
Quintana
María C
MC
Touz
Hugo D
HD
Luján
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000940:Antigenic Variation; D000953:Antigens, Protozoan / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics; D015870:Gene Expression; D017125:Genes, Protozoan; D016829:Giardia lamblia / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D008969:Molecular Sequence Data; D015800:Protozoan Proteins; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C072464:variant surface protein H7, Giardia lamblia