Intracellular redox regulation by a cystine derivative suppresses UV-induced NF-kappa B activation.
Nuclear factor (NF)-kappa B pathways are influenced by the intracellular reduction-oxidation (redox) balance. While NF-kappa B is activated through inhibitor (I)-kappa B degradation by oxidative stress, its DNA binding is accelerated in the reduced state. We found that N,N'-diacetyl-L-cystine dimethylester (DACDM) suppressed the UVB-induced NF-kappa B binding activity at a much lower concentration (50-100 microM) than N-acetyl-L-cysteine (NAC, 10-30 mM). While NAC suppressed the I-kappa B degradation but not the DNA binding, DACDM prevented the activated NF-kappa B from binding DNA, without influencing the I-kappa B degradation. These properties of DACDM make it possible to effectively regulate the intracellular redox balance.
AminoScience Laboratories, Ajinomoto Co., Inc., Suzuki-cho 1-1, Kawasaki-ku, Kawasaki 210-8681, Japan.
Manabu
M
Kitazawa
Takashi
T
Nakano
Hiromi
H
Chuujou
Eiji
E
Shiojiri
Keiji
K
Iwasaki
Kazutami
K
Sakamoto
D000111:Acetylcysteine / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D003553:Cystine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism / Q000528:radiation effects; D005978:Glutathione / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D016328:NF-kappa B / Q000378:metabolism / Q000528:radiation effects*; D010084:Oxidation-Reduction; D014466:Ultraviolet Rays
D000975:Antioxidants; D016328:NF-kappa B; D003553:Cystine; D005978:Glutathione; D019803:Glutathione Disulfide; D000111:Acetylcysteine