Diagnostic techniques to detect cryptic leishmaniasis in dogs.
This study of several techniques for detecting cryptic leishmaniasis in dogs from areas in Spain where Leishmania infantum is highly endemic concludes that immunological techniques (enzyme-linked immunosorbent assay, immunofluorescence antibody test, Western blotting, delayed-type hypersensitivity reaction, and in vitro lymphocyte proliferation assay) do not clearly differentiate between noninfected and infected asymptomatic dogs and that culture and PCR are more reliable diagnostic tools.
Iniesta
Laura
L
Laboratori de Parasitologia, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain.
Fernández-Barredo
Salceda
S
Bulle
Béatrice
B
Gómez
M Teresa
MT
Piarroux
Renaud
R
Gállego
Montserrat
M
Alunda
José M
JM
Portús
Montserrat
M
D000818:Animals; D015153:Blotting, Western; D002353:Carrier State; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006968:Hypersensitivity, Delayed / Q000175:diagnosis / Q000469:parasitology; D018314:Leishmania infantum / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000175:diagnosis* / Q000276:immunology