Anti-platelet effects of different phenolic compounds from Yucca schidigera Roezl. bark.
Resveratrol (3,4',5-trihydroxystilbene) has been reported to have a variety of anti-inflammatory, anti-carcinogenic, anti-fungal and anti-platelet effects. It occurs naturally in different medicinal plants. Recently, resveratrol and other related phenolic compounds including trans-3,3',5,5'-tetrahydroxy-4'-methoxystilbene and yuccaols A and C were isolated from the bark of Yucca schidigera. The aim of the present study was to evaluate in vitro the effects of these compounds on platelet aggregation induced by thrombin and ADP. Pretreatment of platelets with resveratrol or other tested phenolics (1-25 microg/ml) slightly reduced platelet aggregation stimulated by 5 microM ADP (P < 0.05) or 10 microM ADP (P < 0.005). The comparison of the inhibitory effects of tested compound in thrombin-induced platelet aggregation revealed that phenolic showed even stronger antiplatelet actions than resveratrol. These compounds also had an inhibitory effect on the thrombin-induced enzymatic platelet lipid peroxidation determined as the level of thiobarbituric acid reactive substances.
Olas
Beata
B
Department of General Biochemistry, Institute of Biochemistry, University of Lodz, Poland. olasb@taxus.biol.uni-lodz.pl
Wachowicz
Barbara
B
Stochmal
Anna
A
Oleszek
Wieslaw
W
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D004353:Drug Evaluation, Preclinical; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D024301:Plant Bark / Q000737:chemistry; D028321:Plant Preparations / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013552:Swine; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology; D027623:Yucca / Q000737:chemistry*
D010636:Phenols; D028321:Plant Preparations; D010975:Platelet Aggregation Inhibitors; D013267:Stilbenes; D017392:Thiobarbituric Acid Reactive Substances; D000244:Adenosine Diphosphate; D013917:Thrombin; D000077185:Resveratrol