Characterization of a monoclonal antibody specific for a novel primate cell surface marker with distinct biochemical properties on human erythroleukemia and myeloid cell lines.
A hybridoma, CSC-1, which secretes monoclonal antibody (MAb) specific for a cell surface molecule on African green monkey kidney cell line, BGMK, was isolated and characterized. The cell surface molecule recognized by CSC-1 is widely expressed on a variety of human cell lines. Among the hematopoietic cell lines examined, the CSC-1 marker seems to be preferentially expressed by lymphoid cell lines (e.g., Raji, CEM-SS, Jurkat, and MOLT-3). Although CSC-1 also recognizes some uncommitted myeloid and erythroleukemia cell lines (e.g., U-937 and K562), the CSC-1 marker expression on these cell lines is subjected to PMA-induced down-regulation. In addition, the PMA-induced cell surface down-regulation of the CSC-1 marker required more than 24 h. Proteolytic analysis shows that the CSC-1 marker can be categorized into proteinase K-resistant and-sensitive phenotypes. Furthermore, the CSC-1 marker exhibits a slow cell surface turnover rate after proteinase K treatment. Our data suggest that the CSC-1 cell surface molecule might be useful as a megakaryocytic or monocytic differentiation marker.
The John P. Robarts Research Institute, and Department of Microbiology and Immunology, The University of Western Ontario, London, Ontario, N6G 2V4 Canada.
Chew Shun
CS
Chang
Grant
G
McFadden
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015415:Biomarkers; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology* / Q000378:metabolism*; D002522:Chlorocebus aethiops; D019286:Endopeptidase K / Q000378:metabolism; D000939:Epitopes / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D000939:Epitopes; D019286:Endopeptidase K; D013755:Tetradecanoylphorbol Acetate