Crystallization and preliminary crystallographic studies of pyridoxal kinase from sheep brain.
Pyridoxal kinase (ATP:pyridoxal 5'-phosphotransferase; EC 2.7.1.35) is a key enzyme in the transformation of vitamin B(6) to pyridoxal-5'-phosphate. Pyridoxal-5'-phosphate is the crucial cofactor required by numerous enzymes involved in the metabolism of amino acids and the synthesis of many neurotransmitters. Pyridoxal kinase from sheep brain was crystallized in an orthorhombic form using the hanging-drop vapour-diffusion method with sodium citrate as the precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 59.8, b = 94.4, c = 128.2 A, and diffract to a resolution of 2.1 A. Crystals were transferred into a soaking liquid without citrate and two heavy-atom derivatives were prepared.
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, People's Republic of China.
Ming-Hui
MH
Li
Francis
F
Kwok
Xiao-Min
XM
An
Wen-Rui
WR
Chang
Chi-Kong
CK
Lau
Ji-Ping
JP
Zhang
Sheng-Quan
SQ
Liu
Yun-Chung
YC
Leung
Tao
T
Jiang
Dong-Cai
DC
Liang
D000818:Animals; D001921:Brain / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D012756:Sheep