Effects of abomasal vegetable oil infusion on splanchnic nutrient metabolism in lactating dairy cows.
Changes in the metabolism of nutrients by the portal-drained viscera (PDV) and liver may contribute to the reduction in dry matter intake (DMI) and other production responses generally observed in lactating dairy cows fed supplemental long-chain fatty acids (LCFA). In the present study, effects of a 7-d abomasal infusion of vegetable oil on arterial concentration and splanchnic (PDV and liver) metabolism of nutrients were measured in six cows at 55 (early lactation [ELAC]) and 111 (midlactation [MLAC]) d postpartum. Cows were fed for ad libitum DMI at 8-h intervals, and blood samples for measurement of splanchnic metabolism were obtained over 8 h beginning 2 h before feeding at 0830 h. Blood flow for the PDV and liver was increased by oil infusion and was greater in ELAC, despite similar-feed DMI during blood sampling. Increased blood flow in ELAC was associated with greater liver oxygen removal and glucose release that accompanied greater milk yield. In contrast, oil infusion had no effect on splanchnic oxygen use. Greater blood flow during oil infusion may have been due to specific effects of intestinal LCFA supply on PDV blood flow. Net PDV release and liver removal of branched-chain volatile fatty acids (VFA) and ammonia were increased by oil infusion. Net PDV release of longer-chain (4 and 5 C) VFA and NEFA was greater in ELAC, but net PDV flux of other nutrients was not affected by lactation stage, possibly due to the similarity of feed DMI. Oil infusion increased arterial concentration and net PDV release and liver removal of NEFA, and it decreased net liver release and arterial concentration of glucose. Effects of oil infusion on liver glucose release were associated with decreased daily DMI. In ELAC, arterial concentration and net liver removal of NEFA were also increased, but liver release of glucose was greater than in MLAC. Oil infusion and ELAC both increased net liver removal of L-lactate. The resulting decrease in net total splanchnic release of L-lactate in ELAC reflects decreased tissue energy balance of the cows. Generally, stage of lactation and relative milk yield had greater effects on metabolism of the liver than the PDV, in which metabolism was largely dictated by DMI. In the present study, there was little evidence to suggest an effect of stage of lactation on the metabolic response ofthe PDV and liver to postruminal LCFA supply.
Centre for Dairy Research, Department of Agriculture, The University of Reading, England.
J A
JA
Benson
C K
CK
Reynolds
P C
PC
Aikman
B
B
Lupoli
D E
DE
Beever
D000018:Abomasum / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D001784:Blood Gas Analysis / Q000662:veterinary; D002417:Cattle / Q000378:metabolism / Q000502:physiology*; D005227:Fatty Acids / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005260:Female; D006400:Hematocrit / Q000662:veterinary; D007263:Infusions, Parenteral / Q000662:veterinary; D007774:Lactation / Q000378:metabolism*; D008099:Liver / Q000098:blood supply / Q000378:metabolism*; D008892:Milk / Q000378:metabolism; D010938:Plant Oils / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D011168:Portal System / Q000502:physiology; D012039:Regional Blood Flow / Q000187:drug effects; D014508:Urea / Q000378:metabolism; D014781:Viscera / Q000098:blood supply / Q000378:metabolism*
D005227:Fatty Acids; D005232:Fatty Acids, Volatile; D010938:Plant Oils; D000641:Ammonia; D014508:Urea