Genetic diversity within human erythroviruses: identification of three genotypes.
B19 virus is a human virus belonging to the genus Erythrovirus: The genetic diversity among B19 virus isolates has been reported to be very low, with less than 2% nucleotide divergence in the whole genome sequence. We have previously reported the isolation of a human erythrovirus isolate, termed V9, whose sequence was markedly distinct (>11% nucleotide divergence) from that of B19 virus. To date, the V9 isolate remains the unique representative of a new variant in the genus Erythrovirus, and its taxonomic position is unclear. We report here the isolation of 11 V9-related viruses. A prospective study conducted in France between 1999 and 2001 indicates that V9-related viruses actually circulate at a significant frequency (11.4%) along with B19 viruses. Analysis of the nearly full-length genome sequence of one V9-related isolate (D91.1) indicates that the D91.1 sequence clusters together with but is notably distant from the V9 sequence (5.3% divergence) and is distantly related to B19 virus sequences (13.8 to 14.2% divergence). Additional phylogenetic analysis of partial sequences from the V9-related isolates combined with erythrovirus sequences available in GenBank indicates that the erythrovirus group is more diverse than thought previously and can be divided into three well-individualized genotypes, with B19 viruses corresponding to genotype 1 and V9-related viruses being distributed into genotypes 2 and 3.
Laboratoire de Virologie, Hôpital Armand Trousseau, EA2391, UFR Saint-Antoine, Paris, France.
Annabelle
A
Servant
Syria
S
Laperche
Francis
F
Lallemand
Valérie
V
Marinho
Guillemette
G
De Saint Maur
Jean François
JF
Meritet
Antoine
A
Garbarg-Chenon
D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D019218:Erythrovirus / Q000145:classification* / Q000235:genetics*; D005602:France / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000453:epidemiology* / Q000821:virology; D016732:Parvovirus B19, Human; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D000914:Antibodies, Viral; D004279:DNA, Viral