AtCOX17, an Arabidopsis homolog of the yeast copper chaperone COX17.
We have identified a new plant gene, AtCOX17, encoding a protein that shares sequence similarity to COX17, a Cu-binding protein from yeast (Saccharomyces cerevisiae) and vertebrates that mediates the delivery of Cu to the mitochondria for the assembly of a functional cytochrome oxidase complex. The newly characterized Arabidopsis protein has six Cys residues at positions corresponding to those known to coordinate Cu binding in the yeast homolog. Moreover, we show that the Arabidopsis COX17 cDNA complements a COX17 mutant of yeast restoring the respiratory deficiency associated with that mutation. These two lines of evidence indicate that the plant protein identified here is a functional equivalent of yeast COX17 and might serve as a Cu delivery protein for the plant mitochondria. COX17 was identified by investigating the hypersensitive response-like necrotic response provoked in tobacco (Nicotiana tabacum) leaves after harpin inoculation. AtCOX17 expression was activated by high concentrations of Cu, bacterial inoculation, salicylic acid treatment, and treatments that generated NO and hydrogen peroxide. All of the conditions inducing COX17 are known to inhibit mitochondrial respiration and to produce an increase of reactive oxygen species, suggesting that gene induction occurs in response to stress situations that interfere with mitochondrial function.
Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Campus Universidad Autónoma, Cantoblanco, E-28049 Madrid, Spain.
Teresa
T
Balandin
Carmen
C
Castresana
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000494:pharmacology; D001483:Base Sequence; D019256:Cadmium Chloride / Q000494:pharmacology; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D003300:Copper / Q000378:metabolism; D019327:Copper Sulfate / Q000494:pharmacology; D000079922:Copper Transport Proteins; D003576:Electron Transport Complex IV / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009569:Nitric Oxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D020156:Salicylic Acid / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000187:drug effects; D015533:Transcriptional Activation; D017967:Zinc Compounds / Q000494:pharmacology
D029681:Arabidopsis Proteins; C468565:AtCOX17 protein, Arabidopsis; D001425:Bacterial Outer Membrane Proteins; C433183:CCH protein, Arabidopsis; C100359:COX17 protein, S cerevisiae; D027682:Cation Transport Proteins; D002712:Chlorides; D000079922:Copper Transport Proteins; D018832:Molecular Chaperones; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D017967:Zinc Compounds; C075462:harpin protein, Erwinia amylovora; C087295:HrpZ protein, Pseudomonas syringae; D009569:Nitric Oxide; D003300:Copper; C016837:zinc chloride; D006861:Hydrogen Peroxide; D003576:Electron Transport Complex IV; D019256:Cadmium Chloride; D019327:Copper Sulfate; D020156:Salicylic Acid