[Synergistic interaction between Ys-96, a bisbenzylisoquinoline compound derived from Stephania tetrandra, and adriamycin or vincristine against human cancer cell lines in vitro].
OBJECTIVE
To study in vitro the anticancer interaction between Ys-96, a bisbenzylisoquinoline compound derived from Stephania tetrandra, and adriamycin or vincristine against human cancer cell lines.


METHODS
Using human breast cancer cell MCF-7 and its adriamycin-resistant cell line MCF-7/Adr, and human nasopharyngeal cancer cell KB and its vincristine-resistant cell line KBv200 in an in vitro system, anticancer interaction between Ys-96 and adriamycin or vincristine was evaluated with a method reported by Berenbaum.


RESULTS
The SFIC values (sum of fractional inhibitory concentration) of the combinations with 3 different ratios between Ys-96 and adriamycin or vincristine were markedly less than 1.0, and the shapes of all the isobologram curves were concave.


CONCLUSIONS
The synergistic interaction between Ys-96 and adriamycin or vincristine against the above human cancer cell lines was positively observed in vitro.
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700.
Z
Z
Ye
A
A
Sun
C
C
Li
B
B
Xue
A
A
Liang
J
J
Wang
Z
Z
Wang
Y
Y
Tong
H
H
Feng
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D044182:Benzylisoquinolines; D001943:Breast Neoplasms / Q000473:pathology; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D007546:Isoquinolines / Q000302:isolation & purification / Q000494:pharmacology*; D009062:Mouth Neoplasms / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D031609:Stephania tetrandra / Q000737:chemistry*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014750:Vincristine / Q000008:administration & dosage / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D044182:Benzylisoquinolines; D004365:Drugs, Chinese Herbal; D007546:Isoquinolines; D014750:Vincristine; D004317:Doxorubicin