Structure of the O-polysaccharide of Aeromonas hydrophila O:34; a case of random O-acetylation of 6-deoxy-L-talose.
The O-polysaccharide of Aeromonas hydrophila O:34 was obtained by mild-acid degradation of the lipopolysaccharide and studied by chemical methods and NMR spectroscopy before and after O-deacetylation. The polysaccharide was found to contain D-Man, D-GalNAc and 6-deoxy-L-talose (L-6dTal), and the following structure of the tetrasaccharide repeating unit was established [carbohydrate structure see text] where 6dTal(I) is O-acetylated stoichiometrically at position-2 and 6dTal(II) carries no, one or two O-acetyl groups at any positions.
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, Moscow 119991, Russia. knirel@ioc.ac.re
Yuriy A
YA
Knirel
Alexander S
AS
Shashkov
Sof'ya N
SN
Senchenkova
Susana
S
Merino
Juan M
JM
Tomás
D000107:Acetylation; D016980:Aeromonas hydrophila / Q000737:chemistry*; D002240:Carbohydrate Sequence; D003837:Deoxy Sugars / Q000737:chemistry* / Q000378:metabolism*; D006601:Hexoses / Q000737:chemistry* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*
D003837:Deoxy Sugars; D006601:Hexoses; D019081:O Antigens; C011754:6-deoxytalose