Red blood cells imaging and antigen-antibody interaction measurement.
In the present study the atomic force microscope (AFM) was used to image the surface morphology of red blood cells (RBC) for the first time. The AFM yielded very reproducible images without appreciable modifications of the sample surfaces. In addition to this topographical imaging, we have developed an experimental approach to measure the binding strength between antibody (anti-A), and the RBC antigen A, when reversible bonds between specific molecules such as antigen and antibody mediate the adhesion. The experimental results suggest that the procedure established here may be used for specific antibody detection. This study has also enhanced our understanding under physiological conditions of molecular interaction in particular antigen-antibody.
Touhami
A
A
Laboratoire de Physique des Interfaces, Faculté des Sciences de Monastir, Monastir, Tunisia.
Othmane
A
A
Ouerghi
O
O
Ouada
H Ben
HB
Fretigny
C
C
Jaffrezic-Renault
N
N
D000017:ABO Blood-Group System / Q000276:immunology / Q000648:ultrastructure; D000936:Antigen-Antibody Complex / Q000648:ultrastructure*; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D004912:Erythrocytes / Q000276:immunology* / Q000648:ultrastructure*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*
D000017:ABO Blood-Group System; D000936:Antigen-Antibody Complex