Characterization of a fungal protein kinase from Cryphonectria parasitica and its transcriptional upregulation by hypovirus.
The chestnut blight fungus Cryphonectria parasitica and its hypovirus comprise useful model system to study the mechanisms of hypoviral infection. We used degenerate primers based on fungal protein kinases to isolate a gene, cppk1, which encodes a novel Ser/Thr protein kinase of C. parasitica. The gene showed highest homology to ptk1, a Ser/Thr protein kinase from Trichoderma reesei. The encoded protein had a predicted mass of 70.5 kDa and a pI of 7.45. Northern blot analyses revealed that the cppk1 transcript was expressed from the beginning of culture, with a slight increase by 5 days of culture. However, its expression was specifically affected by the presence of virus, and it was transcriptionally upregulated in the fungal strain infected with the hypovirus. A kinase assay using Escherichia coli-derived CpPK1 revealed CpPK1-specific phosphorylated proteins with estimated masses of 50 kDa and 44 kDa. In addition, the phosphorylation of both proteins was higher in a cell-free extract from the hypovirulent strain. The increased expression of cppk1 by the introduction of an additional copy results in a subset of viral symptoms of reduced pigmentation and conidiation in a virus-free isolate. cppk1 overexpression also causes the downregulation of mating factor genes Mf2/1 and Mf2/2, resulting in female sterility. The present study suggests that the hypovirus disturbs fungal signalling by transcriptional upregulation of cppk1, which results in reduced pigmentation and conidiation and female sterility.
Kim
Myoung-Ju
MJ
Institute of Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University, Chonbuk, Korea.
Choi
Jin-Won
JW
Park
Seung-Moon
SM
Cha
Byeong-Jin
BJ
Yang
Moon-Sik
MS
Kim
Dae-Hyuk
DH
D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology; D005658:Fungi / Q000201:enzymology*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012328:RNA Viruses / Q000502:physiology*; D013379:Substrate Specificity; D014158:Transcription, Genetic; D015854:Up-Regulation
D017931:DNA Primers; D011494:Protein Kinases