A major substrate for MPF: cDNA cloning and expression of polypeptide chain elongation factor 1 gamma from goldfish (Carassius auratus).
One of the eukaryotic polypeptide chain elongation factors, EF-1 beta gamma delta complex, is involved in polypeptide chain elongation via the GDP/GTP exchange activity of EF-1 alpha. In the complex, EF-1 gamma has been reported to be a major substrate for maturation promoting factor (MPF). Here, we present the cloning, sequencing and expression analysis of goldfish, Carassius auratus, EF-1 gamma from the goldfish ovary. The cloned cDNA was 1490 bp in length and encoded 442 amino acids. The deduced amino acid sequence was highly homologous to EF-1 gamma from other species. Although, the phosphorylation site identified in Xenopus EF-1 gamma was not conserved in the goldfish homologue, phosphorylation analysis showed that the goldfish EF-1 gamma was phosphorylated by MPF. We concluded that EF-1 gamma is a substrate for MPF during oocyte maturation in goldfish.
Tokumoto
Mika
M
Faculty of Science, Department of Biology and Geosciences, Shizuoka University, Shizuoka 422-8529, Japan.
Nagahama
Yoshitaka
Y
Tokumoto
Toshinobu
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006054:Goldfish / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016200:Maturation-Promoting Factor / Q000378:metabolism*; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
D020648:Peptide Elongation Factor 1; D016200:Maturation-Promoting Factor