The genetic organization of a 2,966 basepair DNA fragment of a single capsid nucleopolyhedrovirus isolated from Trichoplusia ni.
In order to investigate the genomic organization of the Trichoplusia ni Single Capsid Nucleopolyhedrovirus (TnSNPV), a 2,966 basepairs (bp) genomic fragment was sequenced. The fragment was found to contain five open reading frames (ORFs) homologous to baculovirus genes, including p26, fibrillin (p10), AcMNPV ORF-29, late expression factor 6 (lef-6) and the C-terminal portion of p74, on either strand of DNA. Predicted amino acid sequences for the ORFs were compared and identity values of between 12% and 54% were observed. TnSNPV has previously been tentatively identified as a member of the Group II NPVs. Clustering and arrangement of the TnSNPV genes were similar to the clustering reported for SeMNPV, confirming TnSNPV as a Group II NPV.
Department of Microbiology, University of the Western Cape, Bellville, South Africa. bfielding@uwc.ac.za
Burtram Clinton
BC
Fielding
Sehaam
S
Khan
Weizhou
W
Wang
Courtney
C
Kruger
Rayaana
R
Abrahams
Sean
S
Davison
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000235:genetics*; D017924:Nucleopolyhedroviruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D016415:Sequence Alignment; D018411:Spodoptera / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D019590:Nucleocapsid Proteins; D014764:Viral Proteins; C078072:p10 protein, Baculovirus