Receptor component protein (RCP): a member of a multi-protein complex required for G-protein-coupled signal transduction.
The calcitonin-gene-related peptide (CGRP) receptor component protein (RCP) is a 148-amino-acid intracellular protein that is required for G-protein-coupled signal transduction at receptors for the neuropeptide CGRP. RCP works in conjunction with two other proteins to constitute a functional CGRP receptor: calcitonin-receptor-like receptor (CRLR) and receptor-activity-modifying protein 1 (RAMP1). CRLR has the stereotypical seven-transmembrane topology of a G-protein-coupled receptor; it requires RAMP1 for trafficking to the cell surface and for ligand specificity, and requires RCP for coupling to the cellular signal transduction pathway. We have made cell lines that expressed an antisense construct of RCP and determined that CGRP-mediated signal transduction was reduced, while CGRP binding was unaffected. Furthermore, signalling at two other endogenous G-protein-coupled receptors was unaffected, suggesting that RCP was specific for a limited subset of receptors.
Department of Physiology and Biophysics, University of Miami School of Medicine, P.O. Box 016430, Miami, FL 33101, USA.
M A
MA
Prado
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B
Evans-Bain
I M
IM
Dickerson
D000595:Amino Acid Sequence; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000502:physiology*; D017124:Conserved Sequence; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000737:chemistry / Q000502:physiology; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000502:physiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018015:Receptors, Calcitonin Gene-Related Peptide; D011956:Receptors, Cell Surface; D015740:Calcitonin Gene-Related Peptide