Growth and cell cycle regulation by isoflavones in human breast carcinoma cells.
The isoflavones daidzein and biochanin A induced a biphasic growth response in T-47D human breast cancer cells. At growth stimulatory concentrations, daidzein increased the percentage of cells entering the S phase, while at a growth inhibitory concentration, daidzein obstructed the progression of the cell cycle in the G2/M phase. Biochanin A regulated the cell cycle progression in a similar manner and showed a delay in the progression from the S phase to the G2/M phase at growth inhibitory concentrations. The levels of a cell cycle regulatory protein, P53, in response to the treatment of isoflavones, were also determined. Cells that became de-attached and floated in the medium after treatment with growth inhibitory concentrations of daidzein or biochanin A, showed higher P53 levels than cells that remained attached. These results suggest that daidzein and biochanin A influence T-47D cell proliferation and cell cycle progression, and that the underlying mechanisms might be associated with the P53 protein levels.
Ying
Chingwen
C
Department of Microbiology, Soochow University, Taipei, Taiwan, Republic of China. cying@mail.scu.edu.tw
Hsu
Jih-Tay
JT
Hung
Hsiao-Chiao
HC
Lin
Don-Hei
DH
Chen
Long-Fang Oliver
LF
Wang
Lu-Kai
LK
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy; D002277:Carcinoma / Q000188:drug therapy; D002453:Cell Cycle / Q000187:drug effects*; D016195:G2 Phase; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects*; D016196:S Phase; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D016588:Anticarcinogenic Agents; D007529:Isoflavones; D016159:Tumor Suppressor Protein p53; C004742:daidzein; D019833:Genistein; C004541:biochanin A