[Establishment of segregating inbred strain of Yuyi hairless mice and its monitoring of genetic characteristics].
Using brother-sister inbreeding with forced heterozygosity to breed a new segregating inbred strain which carry mutant hairless gene. Then, genetic monitoring was conducted by the skin grafting test, coat color test and biochemical marker analysis, and its basic biological characteristics were studied with corresponding methods. The Results are that the Yuyi Hairless Mice (YYHL) with unique biological characteristics have been bred successfully and have progressed to 30th generation since 1991. Thirteen biochemical markers loci on nine chromosomes coding biochemical markers measured with electrophoresis were all homogenous. The skin grafting test showed that no dropping graft was found during 100 days after transplanting, implying the YYHL was of isohistogeneicity. Analysis of coat color genes indicated that the hair color of first generation hybrid crossed between YYHL and DBA/2 was all agouti suggesting that the coat color genes of the YYHL were homogenous. The gene type is AABBccDD. All these showed that the YYHL have been an inbred strain reaching the international standards.
Zhang
Jin-Tao
JT
Laboratory Animal Center of Medical College of Zhengzhou University, Zhengzhou 450052, China.
Wang
Chun-Yao
CY
Kang
Qiao-Zhen
QZ
Du
Xian-Tang
XT
Xue
Jing-Li
JL
D000818:Animals; D005260:Female; D006200:Hair Color / Q000235:genetics; D008297:Male; D051379:Mice; D008812:Mice, Hairless / Q000235:genetics*; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D016038:Skin Transplantation