Utilization of liposomes in vesicular transport studies.
Various coated vesicles are implicated in the intracellular transport between different compartments. In vitro reconstitution is a powerful experimental system to study molecular mechanisms involved in assembly of coat proteins from cytosol onto membranes as well as formation of coated vesicles. Liposomes have been recently utilized in the cell-free systems. In this review, we summarize studies on reconstitutions of coated vesicles or coated structures on liposomes. A novel method using dynamic light scattering (DLS) to quantify vesicle formation from liposomes also is described. Our recent study on the role of phospholipids in vesicle formation, where the DSL assay is used in combination with lipid analysis, also is introduced.
Department of Neuroscience, Okayama University Graduate School of Medicine and Dentistry, Japan.
Masahiro
M
Kinuta
Kohji
K
Takei
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002451:Cell Compartmentation / Q000502:physiology*; D018699:Coated Vesicles / Q000502:physiology*; D003584:Cytological Techniques; D006801:Humans; D008081:Liposomes; D010743:Phospholipids / Q000378:metabolism; D021381:Protein Transport / Q000502:physiology
D008081:Liposomes; D010743:Phospholipids