A further step towards single-molecule sequencing: Escherichia coli exonuclease III degrades DNA that is fluorescently labeled at each base pair.
Applied Molecular Evolution, Institute for Zoology, University of Leipzig, Talstrasse 33, 04103 Leipzig, Germany. sbrakma@rz.uni-leipzig.de
Susanne
S
Brakmann
Sylvia
S
Löbermann
D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology*; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005456:Fluorescent Dyes; D006868:Hydrolysis; D012235:Rhodamines; D017422:Sequence Analysis, DNA / Q000379:methods*; D013696:Temperature
D005456:Fluorescent Dyes; D012235:Rhodamines; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III