Functional interaction between human papillomavirus type 18 E2 and poly(ADP-ribose) polymerase 1.
Human papillomavirus E2 protein is a transcription factor of viral gene expression and DNA replication. Here we show that PARP is a positive regulator of the E2 protein of human papillomavirus type 18 (HPV-18). PARP interacted with the COOH terminal region of HPV-18 E2 in vitro. The E2 interaction domain within PARP is located in the NH(2)-terminal zinc finger motif and the BRCT motif included in the automodification domain. Overexpression of either wild type or the NH(2)-terminal region of PARP containing zinc finger and BRCT stimulated E2-dependent transcription. Gel retardation assay indicates that PARP augments DNA binding activity of E2 in vitro. We also show that PARP-1 is recruited to E2-dependent promoter in vivo using ChIP assay. These results suggest that PARP serves a transcriptional co-activator in E2-dependent transcription by interacting directly with the HPV E2 protein.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
Daeyoup
D
Lee
Jin Woo
JW
Kim
Karam
K
Kim
Cheol O
CO
Joe
Valérie
V
Schreiber
Josiane
J
Ménissier-De Murcia
Joonho
J
Choe
D019313:BRCA1 Protein / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D006801:Humans; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D016335:Zinc Fingers
D019313:BRCA1 Protein; D009856:Oncogene Proteins, Viral; C069102:oncogene protein E2, Human papillomavirus type 18; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases