Biocompatibility, encrustation and biodegradation of ofloxacine and silver nitrate coated poly-L-lactic acid stents in rabbit urethra.
The purpose of this study was to evaluate the biocompatibility, encrustation and biodegradation properties of silver nitrate and ofloxacine blended caprolactone-L-lactide copolymer coated self-reinforced poly-L-lactic acid (SR-PLLA) urospirals in situ in the male rabbit urethra. SR-PLLA urospirals coated with 10% by weight silver nitrate or 5% by weight ofloxacine blended copolymer or pure copolymer were inserted into the posterior urethra of 18 male rabbits. No prophylactic antibiotics were given. The animals were sacrificed 1 or 6 months after insertion. Urethral tissue reactions were histologically scored semiquantitavely and the appearence of the stents assessed using scanning electron microscopy. The biodegradation time of SR-PLLA stents was remarkably reduced by the caprolactone coating. Silver nitrate and ofloxacine blended copolymer coated urospirals caused less tissue reaction than urospirals with a pure copolymer coating. Silver nitrate coating effectively prevented biofilm formation and stent encrustation. Silver nitrate and ofloxacine blended copolymer coated SR-PLLA urospirals had good biocompatibility properties in rabbit urethra. In particular, coating with silver nitrate may provide possibilities of preventing bacterial adhesion to bioabsorbable stents.
Multanen
Markku
M
Department of Urology, Kuusankoski District Hospital, Kuusankoski, 45750 Sairaalamäki, Finland. am.multanen@pp.fimnet.fi
Tammela
Teuvo L J
TL
Laurila
Marita
M
Seppälä
Jukka
J
Välimaa
Tero
T
Törmälä
Pertti
P
Talja
Martti
M
D000818:Animals; D000892:Anti-Infective Agents, Urinary / Q000494:pharmacology*; D001673:Biodegradation, Environmental / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000378:metabolism* / Q000494:pharmacology*; D003460:Crystallization; D008297:Male; D008855:Microscopy, Electron, Scanning; D015242:Ofloxacin / Q000494:pharmacology*; D011091:Polyesters; D011817:Rabbits; D012835:Silver Nitrate / Q000494:pharmacology*; D015607:Stents; D014521:Urethra / Q000187:drug effects* / Q000648:ultrastructure
D000892:Anti-Infective Agents, Urinary; D020099:Coated Materials, Biocompatible; D011091:Polyesters; C062968:lactide-caprolactone copolymer; D012835:Silver Nitrate; D015242:Ofloxacin