Effect of cyclosporin A treatment on renal calcium oxalate binding in experimental hyperoxaluria.
This study was aimed to investigate the effect ofCyclosporin A administration on renal calcium oxalate binding under hyperoxaluric condition. Cyclosporin A administration or ammonium oxalate treatment increased calcium oxalate binding, which was further increased in kidney treated with cyclosporin A and ammonium oxalate together. The increase of calcium oxalate binding was associated with lipid peroxidation as well as with a concomitant decrease in total thiol in both rat and human kdiney homogenate. Cyclosporin A administration to hyperoxaluric rats resulted with increased calcium oxalate binding protein. However there was no change with specific activity of the protein. In conclusion, Cyclosporin A administration either to normal or hyperoxaluric rats is resulted with increased concentration of calcium oxalate binding protein as well as enhanced activity due to membrane lipid peroxidation.
Department of Medical Biochemistry, Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, India.
Marimuthu
M
Adhirai
Ramasamy
R
Sakthivel
Ramasamy
R
Selvam
D000818:Animals; D002129:Calcium Oxalate / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D016572:Cyclosporine / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D010070:Oxalates / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D013997:Time Factors
D004791:Enzyme Inhibitors; D010070:Oxalates; D002129:Calcium Oxalate; D016572:Cyclosporine