[The expression of Aeromonas hydrophila ompTS gene and the immunogenicity of recombinant OmpTS].
Primers were designed based on ompTS gene reported recently. With the specific primers, one target fragment about 1024 bp lacking the signal sequence of ompTS gene was amplified from A. hydrophila genomic DNA via PCR. The ompTS gene was hyperexpressed using gene fusion expression vector pRSET system, and the recombinant OMP exhibited a size of 39.9 kD with SDS-PAGE and Western blot analysis, which showed about 51% of total lysate proteins. Antibody to the purified recombinant OMP reacted not only to the recombinant OMP but also to the purified OMPs from A. hydrophila in ELISA and the 36.9 kD OMP in Western blot. The result indicates that the recombinant OMP has the same epitope with the nature one.
State Key Lab of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
Jun-Feng
JF
Xie
Qiao-Zhen
QZ
Ye
Jian-Guo
JG
He
Wei-Jun
WJ
Dai
Xiao
X
Huang
Ming-Xiao
MX
He
Cheng
C
Chen
D016980:Aeromonas hydrophila / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology
D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins