Pharmaceutical availability of clobetasol-17-propionate from cream and ointment.
Kinetics of drug release from both compared preparations available as a cream and an ointment, was in vitro studied. A reversed-phase HPLC method was developed for the determination of clobetasol-17-propionate in lipophylic bases using clobetasol-17-butyrate as an internal standard. Analyses were performed using a C18 reversed-phase column with a mobile phase of methanol-water and ultraviolet detection at lambda=254 nm. The calibration curve was constructed for the concentration range 0.5-40.0 microg/ml. The method is simple, accurate and precise.
Department of Clinical Pharmacy, K. Marcinkowski University of Medical Sciences, Poznań, Poland.
S
S
Dyderski
E
E
Grześkowiak
E
E
Szałek
A
A
Mrzygłód
D000287:Administration, Topical; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000493:pharmacokinetics*; D001682:Biological Availability; D002626:Chemistry, Pharmaceutical; D002990:Clobetasol / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics*; D005938:Glucocorticoids; D009824:Ointments
D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D009824:Ointments; D002990:Clobetasol