[PCR sensitivity in detection of DNA of Borrelia burgdorferi sensu lato in different isolates].
OBJECTIVE
Because of lack of standardization of PCR method in Poland to amplify Borrelia burgdorferi s. l. DNA in this case we applied three different primer sets for amplification of: fla gene, 16S rRNA small subunit gene as well as 5S-23S rRNA intergenic spacer and two different polymerases. We compare the efficacy of Borrelia burgdorferi s. l. detection in whole blood from borreliosis suspected patients and isolates of intestine contents of Ixodes ricinus ticks. A quality of polymerase is also important in sensitivity of PCR method.


RESULTS
The best sensitivity of PCR was in blood with primers complementary to 16S rRNA gene, then in ticks isolates with primers complementary to fla gene.


CONCLUSIONS
We affirm that results of PCR detection depend on genetic markers employed to DNA detection and DNA isolation method.
Katedra Genetyki Wydziału Nauk Przyrodniczych, Uniwersytet Szczeciński.
Bogumiła
B
Skotarczak
Beata
B
Wodecka
Teresa
T
Hermanowska-Szpakowicz
D000818:Animals; D015748:Borrelia burgdorferi Group / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D018884:Ixodes / Q000382:microbiology; D008193:Lyme Disease / Q000382:microbiology*; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005819:Genetic Markers; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D012341:RNA, Ribosomal, 5S