Enhanced degradation of an endocrine-disrupting chemical, butyl benzyl phthalate, by Fusarium oxysporum f. sp. pisi cutinase.
Compared to yeast esterase, fungal cutinase degraded butyl benzyl phthalate (BBP) far more efficiently; i.e., almost 60% of the BBP disappeared within 7.5 h. Also, the final chemical composition significantly depended on the enzyme used. Toxicity monitoring using bioluminescent bacteria showed that butyl methyl phthalate, a major product of degradation by esterase, was an oxidative toxic hazard.
Department of Chemical and Biological Engineering, Korea University, Sungbuk-Ku, Seoul 136-701.
Yang-Hoon
YH
Kim
Jeewon
J
Lee
Ji-Young
JY
Ahn
Man Bock
MB
Gu
Seung-Hyeon
SH
Moon
D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D004703:Endocrine System / Q000187:drug effects; D004950:Esterases / Q000378:metabolism; D005670:Fusarium / Q000201:enzymology* / Q000378:metabolism; D049449:Luminescence; D010795:Phthalic Acids / Q000378:metabolism* / Q000494:pharmacology; D015003:Yeasts / Q000201:enzymology
D010795:Phthalic Acids; D004950:Esterases; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; C027561:butylbenzyl phthalate