[Study of monoclonal anti-idiotype antibody that bears the internal image of nasopharyngeal carcinoma associated antigen].
We prepared anti-idiotypic monoclonal antibodies (Ab2) against FC2 and HNL5 that recognized as nasopharyngeal cancer (NPC) associated antigens. These Ab2, which designated as 2H4 and 5D3, could inhibit the binding of FC2 or HNL5 to NPC cell lines. The anti-sera from the immunized mice that contained anti-anti-idiotype antibodies (Ab3) could compete with FC2 or HNL5 for binding with NPC cell by a competitive inhibition assay. When 2H4 or 5D3 immunized mice coupled with keyhole limpet hemocyanin(KLH), positive and specific delayed-type hypersensitivity (DTH) reaction occurred after the mice were stimulated by NPC cells. The proliferative response of the splenocytes was significantly higher in experimental groups than that in control groups. Therefore, it is suggested that the anti-idiotypic antibodies (Ab2) 2H4 and 5D3 are the Ab2 beta bearing the internal image of the epitope of NPC associated antigen, that either 2H4 or 5D3 expressing three dimensional shapes which resemble the structure of natural antigens can induce humoral and cellular immune response.
Li
G C
GC
Cancer Research Institute, Hunan Medical University, Changsha 410078.
Zhou
G H
GH
Zhu
J G
JG
Sun
Q B
QB
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D009303:Nasopharyngeal Neoplasms / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D014407:Tumor Cells, Cultured
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes