[Research on anti-thrombosis effect of ethanol extract from beheaded leech (EEBL)].
OBJECTIVE
To study the anti-thrombosis effect of EEBL and its possible mechanism.


METHODS
Using a thrombosis model induced by collagen-adrenaline in mice and an artery-vein bypass in rats. The effect of EEBL on erythrocyte membrane and thrombocyte membrane fluidity were measured by fluorescence polarization.


RESULTS
EEFL inhibited the thrombosis induced by collagen-adrenaline in mice and the thrombosis in artery-vein bypass in rats significantly. EEBL also enhanced in vitro erythrocyte membrane fluidity and thrombocyte membrane fluidity markedly.


CONCLUSIONS
The anti-thrombosis effect of EEBL may possibly be associated with the enhancement of fluidity of erythrocyte membrane and thrombocyte membrane. There exist anti-thrombosis chemical constituents in the body of beheaded leech.
Guangdong College of Pharmacy, Guangzhou 510224.
Y
Y
Tan
P
P
Xu
X
X
Zhang
S
S
Zhao
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects; D005260:Female; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D007865:Leeches; D008297:Male; D008419:Materia Medica / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D011817:Rabbits; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013927:Thrombosis / Q000517:prevention & control*
D005343:Fibrinolytic Agents; D008419:Materia Medica