Antioxidative effect of ursodeoxycholic acid in the liver of rats with oxidative stress caused by gamma-irradiation.
We studied effects of ursodeoxycholic acid (UDCA) (10 and 100 mg/kg b.w.) on the free radical generation, lipid peroxidation and the antioxidant defense system in the liver of rats with oxidative stress caused by gamma-irradiation. Both doses of UDCA normalized the liver parameters enhanced by gamma-irradiation: the content of superoxide anion and carbonyl-containing products of lipid peroxidation (alkanals, alkenals, alkadienals and ketones), the superoxide dismutase activity and the chemiluminescence enhanced by luminol. Only the highest dose of UDCA (100 mg/kg b.w.) decreased the chemiluminescence enhanced by lucigenin in liver microsomes and the hydroxyalkenals content in the liver. UDCA prevented reduced glutathione depletion caused by gamma-irradiation, whereas glutathione-related enzyme activities did not change under the influence of both the UDCA doses as well as gamma-irradiation. Thus, the data obtained suggest that UDCA is a metabolite having the sufficiently effective antioxidant properties.
Buko
V U
VU
Institute of Biochemistry, National Academy of Sciences, Grodno, Belarus.
Lukivskaya
O Ya
OY
Zavodnik
L V
LV
Sadovnichy
V V
VV
Petushok
N E
NE
Tauschel
N D
ND
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D008163:Luminescent Measurements; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D014580:Ursodeoxycholic Acid / Q000494:pharmacology*
D000975:Antioxidants; D014580:Ursodeoxycholic Acid