pH Change of buffer solution in a microcapillary chip and its suppression.
During the electrophoresis separation of B- and T-cells from lymphocytes employing a microcapillary chip, they were found to become inactive in the reservoir after a short time. This was caused by the buffer solution becoming alkaline due to electrolysis. This was considered to take place in chips with small reservoir volumes. The pH change was confirmed by the ISFET (ion-sensitive field effect transistor) embedded in the chip. To suppress the pH change, two methods were studied. One is the insertion of a salt bridge just in front of and behind the capillary, thus introducing an electric potential but stopping flow of the acid and alkaline solutions into the capillary. The other is neutralization of the alkaline solution in the reservoir by injecting the acid solution produced in another capillary with the same structure by employing an electroosmotic flow (EOF) pump. Both methods achieved no pH change during electrophoresis measurements in the microcapillary.
Department of Materials Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan. oki@micro.mm.t.u-tokyo.ac.jp
Akio
A
Oki
Yuzuru
Y
Takamura
Yoshitaka
Y
Ito
Yasuhiro
Y
Horiike
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002021:Buffers; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D013601:T-Lymphocytes / Q000166:cytology
D002021:Buffers