Isolation and immunochemical characterization of immunoglobulin-binding factor from bovine peripheral blood lymphocytes.
The shedding of immunoglobulin-binding factor (IBF) has been studied in peripheral blood lymphocytes during 1 h incubation at 37 degrees C. IBF was detectable in the incubating medium on the basis of their ability to bind immunoglobulin G (IgG) specifically. IBF was affinity purified on Sepharose beads coated with bovine IgG, fluoresceinated and identified by their biological activities, i.e., for binding immunoglobulin-binding factor labeled with fluoresceinizothiocyanate (FITC-IBF) to erythrocytes coated with purified antibodies (EAIgG) by fluorometric binding assay. The effect of various pH, temperatures and proteolytic enzymes on the binding properties of FITC-IBF to EAIgG was also studied. We showed that IBF are sensitive to pronase E, higher temperature and pH.
Miliukiené
V
V
Institute of Biochemistry, Vilnius, Lietuva.
Dédiniené
J
J
Aleksiené
A
A
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D008214:Lymphocytes / Q000378:metabolism*; D008222:Lymphokines / Q000302:isolation & purification* / Q000378:metabolism; D029584:Prostatic Secretory Proteins
D008222:Lymphokines; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors