Mitogenic potential inducible by He:Ne laser in human lymphocytes in vitro.
The objective of the study was to investigate the mitogenic and genotoxic effects of He:Ne laser irradiation (632.8 nm) on human peripheral lymphocytes in vitro. We used the cytokinesis-block micronucleus assay, which incorporates cytochalasin B to inhibit cytokinesis while karyokinesis proceeds normally leading to the appearance of proliferating lymphocytes as binucleated cells. Also micronuclei will appear in cases of genotoxicologically-affected cells. Buffy coat leukocytes were exposed to 10 mW He:Ne laser at energy densities of 1, 2, 3 and 5 J/cm(2). Cells were then cultured in media 199 without any supplementation for 24, 48, 72 and 96 h adding cytochalasin B 24 h before harvesting of cells. Our results showed that laser-induced lymphocytes proliferate throughout the four consecutive days post-laser irradiation. The difference in the frequency of micronuclei between pre- and post-laser irradiation indicates that a He:Ne laser at such energy densities 1, 2, 3 and 5 J/cm(2) does not induce micronucleus formation. These results shed some light on the mechanism encountered by lymphocytes in the process of He:Ne laser-induced biostimulation.
Department of Surgery, Faculty of Medicine, National Institute for Laser Enhanced Sciences, Cairo University, Egypt. melbatanouny@hotmail.com
Mohamed
M
El Batanouny
Soheir
S
Korraa
Osama
O
Fekry
D001769:Blood / Q000528:radiation effects; D001790:Blood Physiological Phenomena; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000473:pathology / Q000528:radiation effects; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D066298:In Vitro Techniques; D007834:Lasers / Q000009:adverse effects*; D008214:Lymphocytes / Q000473:pathology / Q000528:radiation effects*; D008934:Mitogens / Q000633:toxicity*; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects
D008934:Mitogens; D003571:Cytochalasin B