The effect of commercially available saliva substitutes on predemineralized bovine dentin in vitro.
OBJECTIVE
The objective of this study was to evaluate the effects of various saliva substitutes (Artisial; Glandosane; Oralube; Saliva medac; Oralbalance) on the lesion depth and the mineral content of predemineralized bovine dentin in vitro.


METHODS
Ninety-six dentin samples were prepared from 24 freshly extracted bovine incisors. The samples were predemineralized (pH 5.5; 10 days), and subsequently exposed to the various artificial salivas. A carbonate-reduced mineral water (Hirschquelle) as well as the mouthwash solutions Meridol and biotène served as controls. Lesion depth and mineral loss were evaluated from microradiographs of thin sections (110 microm) by a dedicated software package (TMR 1.24).


RESULTS
After storage in Glandosane and biotène, the results indicated a significant mineral loss and an increase in lesion depth (P < 0.01; Wilcoxon). In contrast, specimens stored in Oralube showed a decrease in lesion depth and an increase in mineral content (P < 0.05; Wilcoxon).


CONCLUSIONS
Thus, the use of neither Glandosane nor biotène can be recommended in dentate patients with severe xerostomia.
Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Freie Universitaet, Berlin, Germany.
H
H
Meyer-Lueckel
J
J
Schulte-Mönting
A M
AM
Kielbassa
D000588:Amines / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000494:pharmacology; D002417:Cattle; D003804:Dentin / Q000187:drug effects* / Q000473:pathology; D004338:Drug Combinations; D005949:Glucose Oxidase / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted; D007784:Lactoperoxidase / Q000494:pharmacology; D008852:Microradiography; D008859:Microscopy, Polarization; D008900:Mineral Waters; D008903:Minerals / Q000032:analysis / Q000494:pharmacology; D009067:Mouthwashes / Q000494:pharmacology; D009113:Muramidase / Q000494:pharmacology; D012464:Saliva, Artificial / Q000145:classification / Q000494:pharmacology*; D012969:Sodium Fluoride / Q000494:pharmacology; D018709:Statistics, Nonparametric; D014002:Tin Fluorides / Q000494:pharmacology; D017001:Tooth Demineralization / Q000473:pathology / Q000503:physiopathology*; D014089:Tooth Remineralization
D000588:Amines; C111669:Biotene; D004338:Drug Combinations; D008900:Mineral Waters; D008903:Minerals; D009067:Mouthwashes; D012464:Saliva, Artificial; D014002:Tin Fluorides; C059813:Meridol; C030296:VA-OraLube; D012969:Sodium Fluoride; D005949:Glucose Oxidase; D007784:Lactoperoxidase; D009113:Muramidase; D002266:Carboxymethylcellulose Sodium