Detection of sialoglycomolecules in five plant trypanosomatids and in an insect phytophagous isolate.
The sialoglycoprotein profiles of five plant trypanosomatids (Phytomonas spp.) and of one flagellate (Herpetomonas sp.) isolated from the salivary gland of a phytophagous insect (Phthia picta) were analyzed by Western blotting using three distinct lectins (LFA, SNA and MAA), which recognize specifically sialic acid residues in glycoconjugates. All six flagellates presented at least one polypeptide recognized by the lectins, with the exception of Phytomonas françai, which did not show any reactivity with SNA agglutinin. Phytomonas serpens and P. françai showed the most distinct pattern of sialoglycoproteins. Phytomonas mcgheei, Herpetomonas sp. and the two other Phytomonas spp., isolated from latex, displayed an identical sialomolecule profile. We discuss the possible role of the sialoglycoproteins in the physiology of these trypanosomatids.
Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes (IMPPG), Centro de Ciências da Saúde (CCS), Bloco I, Universidade do Brasil (UFRJ), Ilha do Fundão, 21941-590 Rio de Janeiro, Brazil.
André Luis
AL
Souza dos Santos
Celuta
C
Sales Alviano
Rosangela Maria
RM
de Araújo Soares
D000818:Animals; D015153:Blotting, Western; D006430:Hemiptera / Q000469:parasitology*; D037102:Lectins; D010944:Plants / Q000469:parasitology*; D012469:Salivary Glands / Q000469:parasitology; D012795:Sialoglycoproteins / Q000032:analysis*; D014351:Trypanosomatina / Q000737:chemistry* / Q000302:isolation & purification
D037102:Lectins; D012795:Sialoglycoproteins