Induction of glutathione-dependent hepatic biotransformation enzymes by lycopene in the hamster cheek pouch carcinogenesis model.
Consumption of carotenoids is recognised to be inversely related with cancer incidence. Lycopene, a major carotenoid in tomatoes is a potent antioxidant with potential anticarcinogenic properties. We undertook the study to investigate the effect of lycopene on hepatic biotransformation enzymes during 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis using hepatic lipid peroxidation, reduced glutathione (GSH) and biotransformation enzymes that use GSH as a substrate such as glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR). Enhanced lipid peroxidation in the liver of tumour-bearing animals was accompanied by significant decreases in the activities of GSH and GSH-dependent enzymes. Administration of lycopene significantly decreased the formation of lipid peroxides and enhanced the activities of hepatic biotransformation enzymes. Our results indicate that elevation of hepatic GSH and biotransformation enzymes by lycopene may play a key role in preventing cancer development at extrahepatic sites.
Faculty of Science, Department of Biochemistry, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India.
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Bhuvaneswari
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Velmurugan
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Nagini
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001711:Biotransformation; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000494:pharmacology; D002338:Carotenoids / Q000737:chemistry / Q000494:pharmacology*; D002610:Cheek; D006224:Cricetinae; D004195:Disease Models, Animal; D005978:Glutathione / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology*; D000077276:Lycopene; D008647:Mesocricetus; D017392:Thiobarbituric Acid Reactive Substances
D000975:Antioxidants; D002273:Carcinogens; D017392:Thiobarbituric Acid Reactive Substances; D002338:Carotenoids; D015127:9,10-Dimethyl-1,2-benzanthracene; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D000077276:Lycopene