Recombinant Rhodococcus sp. strain T09 can desulfurize DBT in the presence of inorganic sulfate.
The putative Rhodococcus rrn promoter region was cloned from the benzothiophene desulfurizing Rhodococcus sp. strain T09, and the dibenzothiophene desulfurizing gene, dsz, was expressed under the control of the putative rrn promoter in the strain T09 using a Rhodococcus-E.coli shuttle vector. Strain T09 harboring the expression vector, pNT, could desulfurize dibenzothiophene in the presence of inorganic sulfate, methionine, or cysteine, while the Dsz phenotype was completely repressed in recombinant cells carrying the gene under the control of the native dsz promoter under the same conditions. Among the sulfur sources examined, no intermediates were detected and only the final desulfurized product, 2-hydroxy-biphenyl, was produced using ammonium sulfate as the sulfur source.
Bio-Refining Process Laboratory, Japan Cooperation Center Petroleum, 1900 Sodeshi-cho Shimizu-shi, Shizuoka 424-0037, Japan. tmatsui@brpl.jccp.or.jp
Toru
T
Matsui
Ken-ichi
K
Noda
Yasuhiro
Y
Tanaka
Kenji
K
Maruhashi
Ryuichiro
R
Kurane
D003001:Cloning, Molecular; D003470:Culture Media; D003545:Cysteine / Q000378:metabolism; D004274:DNA, Recombinant / Q000235:genetics; D008715:Methionine / Q000378:metabolism; D008957:Models, Genetic; D009876:Operon; D011401:Promoter Regions, Genetic; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification; D012240:Rhodococcus / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013431:Sulfates / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*
D003470:Culture Media; D004274:DNA, Recombinant; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D013876:Thiophenes; C088015:benzothiophene; D008715:Methionine; D003545:Cysteine; C016366:dibenzothiophene