Novel optical solid-state glucose sensor using immobilized glucose oxidase.
Meso-tetra(4-carboxyphenyl)porphine (CTPP(4)) binds reversibly to immobilized glucose oxidase (GOD), resulting in an absorbance peak for the CTPP(4)-GOD complex at 427nm. The absorbance intensity of the 427nm peak is reduced upon exposure to glucose, which causes the dissociation of CTPP(4) from GOD. The change in absorbance at 427nm shows linear dependence on glucose concentration from 20 to 200mg/dL (1.1-11.1mM).
White
Brandy J
BJ
Department of Physics, Oklahoma State University, 74078, Stillwater, OK, USA.
James Harmon
H James
HJ
D015374:Biosensing Techniques / Q000379:methods*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D011166:Porphyrins / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*
D011166:Porphyrins; C018395:tetracarboxyphenylporphine; D005949:Glucose Oxidase; D005947:Glucose