Molecular cloning, characterization and mapping of a rhodanese like gene in wheat.
To isolate genes related to resistance to Erysiphe graminis (Blumeria graminis) DC. f. sp. tritici in wheat (Triticum aestivum L.), differential display analysis was conducted for mRNA extracted from seedlings of a wheat-Haynaldia villosa 6VS/6AL translocation line 92R137 that contains a powdery mildew resistance gene Pm21. A full-length cDNA sequence named TaTST (Triticum aestivum thiosulfate sulfurtransferase) homologous to the thiosulfate sulfurtransferase (rhodanese) in Datisca glomerata was isolated. Northern blot showed that the expression of TaTST was enhanced after infection with Erysiphe graminis. TaTST was mapped on the short arm of 6B chromosomes of wheat through Southern blot and GSP-PCR using Chinese Spring nullisomic/tetrasomic lines and ditelosomic lines. There is a homologue of TaTST on 6VS too.
Key Laboratory of Crop Cytogenetics, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. jsniu@263.net
Ji-Shan
JS
Niu
Ling
L
Yu
Zheng-Qiang
ZQ
Ma
Pei-Du
PD
Chen
Da-Jun
DJ
Liu
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017343:Genes, Plant; D008969:Molecular Sequence Data; D013884:Thiosulfate Sulfurtransferase / Q000737:chemistry / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
D013884:Thiosulfate Sulfurtransferase