Functional characterization of Brucella melitensis NorMI, an efflux pump belonging to the multidrug and toxic compound extrusion family.
Two putative proteins (NorMI and NorMII) similar to the multidrug efflux protein NorM of Vibrio parahaemolyticus are encoded by the Brucella melitensis 16 M genome. We show that a drug-hypersusceptible Escherichia coli strain overexpressing NorMI displays increased resistance to norfloxacin, ciprofloxacin, gentamicin, tetraphenylphosphonium ion, acriflavine, and berberine. This elevated resistance was proven to be mediated by an energy-dependent efflux mechanism. NorMI belongs to the multidrug and toxic compound extrusion family and is the first multidrug efflux protein identified in Brucella spp.
Unité de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France.
Martine
M
Braibant
Laurence
L
Guilloteau
Michel S
MS
Zygmunt
D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology*; D001693:Biological Transport, Active; D017347:Brucella melitensis / Q000235:genetics* / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004734:Energy Metabolism / Q000502:physiology; D004926:Escherichia coli / Q000187:drug effects; D016680:Genome, Bacterial; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data
D000900:Anti-Bacterial Agents; D026901:Membrane Transport Proteins