LaPSvS1, a (1-->3)-beta-galactan sulfate and its effect on angiogenesis in vivo and in vitro.
LaPSvS1, a highly sulfated branched (1-->3)-beta-galactan was prepared from the arabino-galactan from Larix decidua Miller by partial hydrolysis and subsequent sulfation with SO(3)-pyridine in DMF. The molecular weight was analyzed by GPC and the sulfate content was determined by ion chromatography. LaPSvS1 exhibited good antiangiogenic and antiinflammatory effects in two different modifications of the known CAM-assay. In vitro results obtained in the FGF-2-trypsin-assay and in fluorospectrometric experiments revealed that LaPSvS1 interacts with the fibroblast growth factor 2 system. This interaction is correlated with the in vivo effect of LaPSvS1 on FGF-2 induced angiogenesis.
Institute of Pharmaceutical Biology, University of Regensburg, Universitätsstrasse 31, D-93040, Regensburg, Germany.
Jutta
J
Bürgermeister
Dietrich H
DH
Paper
Horst
H
Vogl
Robert J
RJ
Linhardt
Gerhard
G
Franz
D000482:Allantois / Q000098:blood supply; D020533:Angiogenesis Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001089:Arabinose / Q000737:chemistry; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology; D005685:Galactans / Q000737:chemistry* / Q000494:pharmacology*; D006493:Heparin / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010946:Plants, Medicinal; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013431:Sulfates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013463:Sulfuric Acid Esters; D013997:Time Factors
D020533:Angiogenesis Inhibitors; D000893:Anti-Inflammatory Agents; D005685:Galactans; C472749:LaPSvS1; D013431:Sulfates; D013463:Sulfuric Acid Esters; D016222:Fibroblast Growth Factor 2; D012967:Sodium Dodecyl Sulfate; D006493:Heparin; D001089:Arabinose