Proton dipolar recoupling in resin-bound peptides under high-resolution magic angle spinning.
Rotational resonance and radiofrequency-driven dipolar recoupling (RFDR) experiments have been used to recover the weak proton dipolar interaction present in peptides bound to swollen resins spun at the magic angle. The intensity of the correlation peaks obtained using these sequences is shown to be significantly stronger than the one obtained using the classical NOESY experiment. In addition, it is found that during the relatively long mixing times required to transfer magnetization in such soft materials, the RFDR sequence also achieves magnetization transfer via the scalar J-coupling.
Raya
Jésus
J
Institut de Chimie, UMR 7510 CNRS-Bruker, Université Louis Pasteur, Strasbourg, France.
Bianco
Alberto
A
Furrer
Julien
J
Briand
Jean-Paul
JP
Piotto
Martial
M
Elbayed
Karim
K
D000595:Amino Acid Sequence; D029544:Foot-and-Mouth Disease Virus / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D012116:Resins, Plant / Q000737:chemistry*
D010455:Peptides; D012116:Resins, Plant