[Optimization of cultivation conditions in se-enriched Spirulina platensis].
Orthogonal combination design was adopted in examining the Spirulina platensis (S. platensis) yield and the influence of four factors (Se content, Se-adding method, S content and NaHCO3 content) on algae growth. The results showed that Se content, Se-adding method and NaHCO3 content were key factors in cultivation conditions of Se-enriched S. platensis with the optimal combination being Se at 300 mg/L, Se-adding amount equally divided into three times and NaHCO3 at 16.8 g/L. Algae yield had a remarkable correlation with OD560 and floating rate by linear regression analysis. There was a corresponding relationship between effects of the four factors on algae yield and on OD560, floating rate too. In conclusion, OD560 and floating rate could be served as yield-forming factors.
Huang
Zhi
Z
Department of Biotechnology, Department of Chemistry, Jinan University, Guangzhou 510632.
Zheng
Wen-Jie
WJ
Guo
Bao-Jiang
BJ
D001639:Bicarbonates / Q000032:analysis; D003470:Culture Media; D000458:Cyanobacteria / Q000254:growth & development*; D012643:Selenium / Q000032:analysis / Q000494:pharmacology*
D001639:Bicarbonates; D003470:Culture Media; D012643:Selenium