Xenon derivatization of halide-soaked protein crystals.
Crystals of porcine pancreatic elastase can be derived by soaking in high-molarity bromide solutions. These crystals, either in a glycerol-based cryoprotectant solution or in paraffin oil, can be subsequently pressurized under a xenon atmosphere to incorporate xenon. When paraffin oil is used, a larger number of bromide ions are observed on the protein surface. Intensity data collected to lower energy than the bromine absorption edge can be used to determine the xenon position and the resultant phase information can be used to determine the bromine substructure from data collected to higher energy than the bromine absorption edge. The method would appear to have general applicability where large substructures need to be determined.
EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, Germany.
Santosh
S
Panjikar
Paul A
PA
Tucker
D000818:Animals; D001965:Bromides / Q000737:chemistry*; D003460:Crystallization; D010196:Pancreatic Elastase / Q000737:chemistry*; D013552:Swine; D014978:Xenon / Q000737:chemistry*
D001965:Bromides; D014978:Xenon; D010196:Pancreatic Elastase