Up-scaling capillary zone electrophoresis separations of polydisperse anionic polyelectrolytes with preparative free-flow electrophoresis exemplified with a soil fulvic acid.
A scale-up of analytical capillary zone electrophoresis (CZE) to preparative free-flow electrophoresis (FFE) is described. FFE allows fractionations based on charge densities in larger amounts than in CZE, enabling further off-line analysis of the fractions. Model compounds (carboxylic acids and polystyrene sulfonates) showed a similar behavior in FFE as in CZE. Diffusion and electrodynamic distortion effects are more pronounced in FFE than in CZE. A soil fulvic acid was analyzed by CZE and fractionated by FFE. A comparison of the FFE fractions with CZE measurements of the same sample using the effective mobility scale showed good agreement of the two methods.
Junkers
Jens
J
Institute for Soil Ecology, Forschungszentrum für Umwelt und Gesundheit, Neuherberg, Germany.
Schmitt-Kopplin
Philippe
P
Munch
Jean-Charles
JC
Kettrup
Antonius
A
D001578:Benzopyrans / Q000032:analysis / Q000302:isolation & purification*; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes; D006812:Humic Substances / Q000032:analysis / Q000302:isolation & purification; D007202:Indicators and Reagents; D000071228:Polyelectrolytes; D011108:Polymers / Q000032:analysis / Q000302:isolation & purification*; D012987:Soil
D001578:Benzopyrans; D005456:Fluorescent Dyes; D006812:Humic Substances; D007202:Indicators and Reagents; D000071228:Polyelectrolytes; D011108:Polymers; D012987:Soil; C005023:fulvic acid