Attenuation of LPS-induced changes in synaptic activity in rat hippocampus by Vasogen's Immune Modulation Therapy.
Systemic injection of lipopolysaccharide (LPS) blocks the expression of long-term potentiation in the hippocampus of the rat. This is coupled with increased IL-1beta concentration and c-Jun NH(2)-terminal kinase activity, as well as an increase in the number of cells displaying apoptotic characteristics in the hippocampus. Vasogen's Immune Modulation Therapy (IMT) is a procedure involving intramuscular administration of syngeneic blood which has been exposed ex vivo to elevated temperature, oxidation and ultraviolet light. We report that Vasogen's IMT significantly abrogates these LPS-induced effects with a concomitant increase in the concentration of the anti-inflammatory cytokine IL-10. These data suggest that Vasogen's IMT may play a protective role against the deleterious effects of immune insults in the brain.
Nolan
Yvonne
Y
Department of Physiology, Trinity College, Dublin, Ireland.
Minogue
Aedín
A
Vereker
Emily
E
Bolton
Anthony E
AE
Campbell
Veronica A
VA
Lynch
Marina A
MA
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D053938:DNA Fragmentation / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000150:complications* / Q000276:immunology / Q000503:physiopathology; D006624:Hippocampus / Q000187:drug effects / Q000276:immunology* / Q000503:physiopathology; D007167:Immunotherapy; D007375:Interleukin-1 / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000276:immunology; D017774:Long-Term Potentiation / Q000187:drug effects / Q000276:immunology*; D008297:Male; D048055:Mitogen-Activated Protein Kinase 8; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009410:Nerve Degeneration / Q000188:drug therapy* / Q000276:immunology* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000187:drug effects / Q000276:immunology*; D014185:Transplantation, Isogeneic
D007375:Interleukin-1; D008070:Lipopolysaccharides; D016753:Interleukin-10; D048055:Mitogen-Activated Protein Kinase 8; D020928:Mitogen-Activated Protein Kinases