Peripheral blood mononuclear cell proliferation to heat shock protein-70 derived from autologous lung carcinoma.
In animals, tumor-derived heat shock proteins (HSP) induce immune-mediated protection against autologous cancer. We investigated whether HSP-70 derived from human lung carcinoma are also complexed to tumor-specific antigens. Peripheral blood mononuclear cells collected 10 days after surgery from patients with lung cancer were stimulated with HSP-70 purified from autologous and heterologous tumors. The stimulation index (SI) obtained when stimulating cells with autologous tumor-derived HSP-70 averaged 3.07 +/- 0.75 in patients with lung cancer and 1.57 +/- 0.33 in control subjects (p < 0.001 by analysis of variance). No significant stimulation was observed with HSP-70 derived either from the majority of heterologous tumors or from autologous tumor-free lung tissue. SI decreased from 3.59 +/- 0.65 to 1.65 +/- 0.38 in six patients tested again 3 months after surgery (p = 0.02 by Wilcoxon test for paired data). HSP-70 derived from lung carcinoma are shown to be associated with T cell antigens. The T cell reactivity appears transient and restricted to antigens complexed to HSP-70 derived from autologous tumors only. This suggests that the antigenicity of human lung tumors is unique, which may be crucial for the design of new vaccines.
Michils
Alain
A
Chest Department, IRIBHN Statistical Unit, Erasme University Hospital, CUB Erasme, Brussels, Belgium. amichils@ulb.ac.be
Dutry
Dominique
D
de Beyl
Valérie Zegers
VZ
Remmelink
Myriam
M
de Maertelaer
Viviane
V
Rocmans
Pierre
P
D000230:Adenocarcinoma / Q000473:pathology; D000368:Aged; D000704:Analysis of Variance; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002455:Cell Division / Q000187:drug effects*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000494:pharmacology*; D006801:Humans; D015151:Immunoblotting; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000502:physiology*; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011336:Probability; D012680:Sensitivity and Specificity; D014182:Transplantation, Autologous; D014407:Tumor Cells, Cultured
D018840:HSP70 Heat-Shock Proteins