Development of an in vitro system detecting pro-embryotoxin.
An in vitro system for detection of embryotoxins has been developed by using primary cultures of embryo fibroblasts. Various embryotoxins, including benzo[a]pyrene and thalidomide, have trivial cytotoxicity in embryo fibroblast systems, which is at least in part due to a lack of capacity for metabolic activation. Introduction of steps for microsomal pre-incubation and calcium-precipitation prior to chemical contact resulted in the clear appearance of embryotoxicity toward thalidomide and benzo[a]pyrene. This pre-incubation method will offer advantages for the detection of embryotoxins, which require maternal metabolic activation, and for understanding the mechanisms of their metabolic activations.
Miyata
Masaaki
M
Division of Drug Metabolism and Molecular Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan. miyata@mail.pharm.tohoku.ac.jp
Tamura
Etsuko
E
Yamazoe
Yasushi
Y
D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000633:toxicity; D001681:Biological Assay / Q000379:methods* / Q000706:statistics & numerical data; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D011817:Rabbits; D013723:Teratogens / Q000378:metabolism* / Q000494:pharmacology*; D013792:Thalidomide / Q000378:metabolism / Q000633:toxicity
D013723:Teratogens; D001564:Benzo(a)pyrene; D013792:Thalidomide