The African swine fever virus protein j4R binds to the alpha chain of nascent polypeptide-associated complex.
The African swine fever virus (ASFV) j4R protein is expressed late during the virus replication cycle and is present in both the nucleus and the cytoplasm of infected cells. By using the yeast two-hybrid system, direct binding, and coprecipitation from cells, we showed that the j4R protein binds to the alpha chain of nascent polypeptide-associated complex (alpha NAC). Confocal microscopy indicated that a proportion of j4R and alpha NAC interact in areas close to the plasma membrane, as well as through the cytoplasm in cells. In vitro binding studies suggested that binding of j4R to alpha NAC did not interfere with the binding of alpha- and beta NAC subunits (the BTF3 transcription factor).
Institute for Animal Health, Pirbright, Woking, Surrey GU24 ONF, United Kingdom.
Lynnette C
LC
Goatley
Steve R F
SR
Twigg
James E
JE
Miskin
Paul
P
Monaghan
René
R
St-Arnaud
Geoffrey L
GL
Smith
Linda K
LK
Dixon
D000358:African Swine Fever Virus / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000737:chemistry; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D016366:Open Reading Frames; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection; D020798:Two-Hybrid System Techniques; D014709:Vero Cells; D014764:Viral Proteins / Q000378:metabolism*
D018832:Molecular Chaperones; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D014764:Viral Proteins; C096099:nascent-polypeptide-associated complex; C063552:transcription factor BTF3