A panel of monoclonal antibodies against human polycomb group proteins.
Polycomb-group (PcG) proteins are chromatin-associated proteins that heritably repress gene activity in many organisms, including man. Two distinct human PcG complexes have been identified. The HPC/HPH PcG complex I contains the HPC, HPH, RING1, and BMI1 proteins, the EED/EZH2 PcG complex II contains the EED, EZH2, and YY1 proteins. Previously we found that the relative expression levels of proteins of the human PcG complexes I and II are severely deregulated in human tumors. These findings signify an important role for antibodies against human PcG proteins as diagnostic tools. To be able to produce standardized anti-human PcG antibodies, we developed a panel of five mouse monoclonal antibodies (MAbs) against the human PcG proteins HPC2, BMI1, RING1A, EED, and EZH2. All MAbs can be used for Western blot analysis and immunofluorescence labeling of tissue culture cells. With the exception of the MAb against HPC2, all MAbs can also be used in immunoprecipitation experiments and immunohistochemistry of human tissues. The novel MAbs are therefore valuable tools for the cell biological, biochemical, and pathological analysis of human PcG proteins.
Swammerdam Institute for Life Sciences, BioCentrum Amsterdam, University of Amsterdam, 1018 TV Amsterdam, The Netherlands.
Karien M
KM
Hamer
Richard G A B
RG
Sewalt
Jan L
JL
den Blaauwen
Thijs
T
Hendrix
David P E
DP
Satijn
Arie P
AP
Otte
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002352:Carrier Proteins / Q000276:immunology; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000276:immunology; D000071221:Enhancer of Zeste Homolog 2 Protein; D005455:Fluorescent Antibody Technique; D018922:HL-60 Cells; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D008025:Ligases; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins / Q000276:immunology; D063150:Polycomb Repressive Complex 1; D063151:Polycomb Repressive Complex 2; D063146:Polycomb-Group Proteins; D011233:Precipitin Tests; D011506:Proteins / Q000276:immunology; D011518:Proto-Oncogene Proteins / Q000276:immunology; D012097:Repressor Proteins / Q000276:immunology*; D014157:Transcription Factors; D044767:Ubiquitin-Protein Ligases
D000911:Antibodies, Monoclonal; C069077:BMI1 protein, human; C489421:Bmi1 protein, mouse; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C491488:EED protein, human; C107275:Eed protein, mouse; D009687:Nuclear Proteins; D063146:Polycomb-Group Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; C498410:Ezh2 protein, mouse; D011495:Histone-Lysine N-Methyltransferase; D063151:Polycomb Repressive Complex 2; D063150:Polycomb Repressive Complex 1; C080355:RING1 protein, human; C489840:Ring1 protein, mouse; D044767:Ubiquitin-Protein Ligases; D008025:Ligases; C570427:CBX4 protein, human