Local levels of interleukin-1beta, -4, -6 and tumor necrosis factor alpha in an experimental model of murine osteomyelitis due to staphylococcus aureus.
The purpose of this study is to evaluate local levels of interleukin-1 beta (IL-1 beta), -4 (IL-4), -6 (IL-6), and tumour necrosis factor-alpha (TNF-alpha), in a model of murine osteomyelitis due to Staphylococcus aureus. Cytokine levels in supernatants derived from bone homogenates were determined by enzyme-linked immunosorbent assay, for 28 days following the direct implantation of murine tibiae with S.aureus. Levels of IL-1 beta and IL-6 in infected bone were elevated in the early post-infection period and then decreased. In contrast, TNF-alpha levels remained elevated 3 to 28 days post-infection, while IL-4 levels were elevated late in the course of infection. The histopathology of infected bone showed predominant infiltration of inflammatory cells and bone resorption 3 to 7 days after infection, and bone resorption and adjacent areas of formation 14 to 28 days after infection. These results suggest that the elevated IL-1 beta and IL-6 levels induced by infection may be related to bone damage mainly in the early phase of infection, and that TNF-alpha and IL-4 may at least in part be associated with histopathological changes, including both bone resorption and formation in the later phase of this osteomyelitis model.
Yoshii
Takashi
T
Department of Dentistry, Hyogo Prefectural Staff Health Center, Kobe, 650-8567, Japan. takashi3@basil.ocn.ne.jp
Magara
Shinya
S
Miyai
Daisuke
D
Nishimura
Hidetaka
H
Kuroki
Eiji
E
Furudoi
Shungo
S
Komori
Takahide
T
Ohbayashi
Chiho
C
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D007375:Interleukin-1 / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D010010:Osteoclasts / Q000378:metabolism; D010019:Osteomyelitis / Q000209:etiology / Q000378:metabolism* / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000150:complications / Q000378:metabolism; D013211:Staphylococcus aureus; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4