Cloning and expression of Xenopus Lrp5 and Lrp6 genes.
LRP5 and LRP6 comprise a subfamily of lipoprotein-receptor related proteins that function as co-receptors for Wnt proteins. Mutation of human LRP5 is responsible for osteoporosis-pseudoglioma syndrome and disruption of Lrp6 in mice causes similar effects to mutation of several different Wnt genes. We have cloned Xenopus homologues of Lrp5 and Lrp6 (Xlrp5, Xlrp6) and examined their expression during embryogenesis. Both genes are expressed maternally and ubiquitously through early development. At later stages, Xlrp5 is found in the eye, forebrain, hindbrain, branchial arches and the tip of the tail bud. Xlrp6 is expressed throughout the central nervous system, branchial arches, in the eye and otic vesicle. Both genes are also expressed at the intersomitic boundary. These results suggest roles for Wnt signaling via LRP proteins in these tissues.
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229-3039, USA.
Douglas W
DW
Houston
Chris
C
Wylie
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D026502:LDL-Receptor Related Proteins; D060492:Low Density Lipoprotein Receptor-Related Protein-5; D060488:Low Density Lipoprotein Receptor-Related Protein-6; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010024:Osteoporosis / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D011973:Receptors, LDL / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D051153:Wnt Proteins; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins
D026502:LDL-Receptor Related Proteins; C555482:LRP5 protein, human; C555475:LRP6 protein, human; D060492:Low Density Lipoprotein Receptor-Related Protein-5; D060488:Low Density Lipoprotein Receptor-Related Protein-6; C555483:Lrp5 protein, mouse; C555476:Lrp6 protein, mouse; D011518:Proto-Oncogene Proteins; D011973:Receptors, LDL; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D004247:DNA