Inhibition of vacuolation toxin activity of Helicobacter pylori by iodine, nitrite and potentiation by sodium chloride, sterigmatocystin and fluoride.
The toxin VacA produced by Helicobacter pylori is an important determinant of virulence. VacA causes vacuolation of cultured cells such as HeLa cells. Iodine, nitrite, sodium chloride, thiocyanate and fungus toxin sterigmatocystin are universally present in nature and could possibly be related to carcinogenesis of the stomach. The present study was designed to examine the effects of the above-mentioned compound on VacA-induced vacuolation of HeLa cells, which was quantitated using the neutral red uptake assay. VacA-induced vacuolation was inhibited by BafA1 and NPPB. Formation of large vacuoles was inhibited in the presence of iodine, nitrite, but enhanced by sodium chloride, thiocyanate, fluoride and sterigmatocystin. Our results indicate that VacA toxin may interact with other gastric cancer risk factors present naturally in the environment, and suggest that those compounds may modulate the development of gastric cancer induced by H. pylori.
Department of Public Health and Hygiene, Oita Medical University, 1-1 Idaigaoka, Hasama-machi, Oita 879-5593, Japan.
Fengjuan
F
Ma
Wenyuan
W
Zhao
Masanobu
M
Kudo
Kazuo
K
Aoki
Junichi
J
Misumi
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005459:Fluorides / Q000494:pharmacology*; D006367:HeLa Cells / Q000187:drug effects; D016480:Helicobacter pylori / Q000737:chemistry / Q000502:physiology; D006801:Humans; D007455:Iodine / Q000494:pharmacology*; D018942:Macrolides; D009573:Nitrites / Q000494:pharmacology*; D009579:Nitrobenzoates / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology*; D013241:Sterigmatocystin / Q000494:pharmacology*; D014617:Vacuoles / Q000187:drug effects
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; D009573:Nitrites; D009579:Nitrobenzoates; C074689:VacA protein, Helicobacter pylori; D013241:Sterigmatocystin; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D012965:Sodium Chloride; C040929:bafilomycin A1; D007455:Iodine; D005459:Fluorides