Effect of p-nitrophenol metabolites on microbial cell electro-optical characteristics.
The effect of cellular p-nitrophenol (PNP) metabolism on the electro-optical (EO) characteristics of Pseudomonas putida C-11, P. putida BA-11, and Acinetobacter calcoaceticum A-122 was studied. When P. putida C-11 was incubated with hydroquinone, the orientational spectra of the cell suspensions changed considerably. When P. putida BA-11 and A. calcoaceticum A-122 were incubated with hydroquinone, no orientational spectrum changes were noted, possibly attesting to the operation of different PNP-metabolic pathways. In C-11, the initial metabolism of PNP may occur via the production of hydroquinone, an intermediate for PNP metabolism; in BA-11 and A. calcoaceticum A-122, via the production of 4-nitropyrocatechin, followed by a rupture of the aromatic ring. The respiratory activity of the strains toward hydroquinone was investigated concurrently. The results suggest that EO analysis is a good candidate for the study of cellular metabolism.
Ignatov
Oleg V
OV
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410015, Russia. oignatov@ibppm.saratov.su
Guliy
Olga I
OI
Shchyogolev
Sergei
S
Bunin
Victor D
VD
Ignatov
Vladimir V
VV
D016954:Acinetobacter calcoaceticus / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D018533:Biomass; D004594:Electrophysiology; D006873:Hydroquinones / Q000378:metabolism; D009596:Nitrophenols / Q000378:metabolism*; D055095:Optics and Photonics; D010101:Oxygen Consumption; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology
D006873:Hydroquinones; D009596:Nitrophenols; C024719:4-nitrophenyl; C031927:hydroquinone