Differentiation between non-Hodgkin's lymphoma recurrence and second primary lymphoma by VDJ rearrangement analysis.
Relapses in non-Hodgkin's lymphomas (NHL) could be due to the reappearance of the initial neoplasm or new primary tumours. Discrimination between the two events would allow a more targeted therapeutic approach. VDJ rearrangement was used as marker of clonality in metachronous biopsy specimens from 10 patients with relapsed B-NHL. Complimentary determining region 3 was amplified and sequenced. D-JH was identical in eight matched primary/secondary tumours, confirming the diagnosis of recurrence. In contrast, primary and secondary tumours in two patients were of different clonal origin. Our data indicate that VDJ analysis is a fundamental tool for identification of relapses in NHL.
Division of Experimental Oncology 1, Centro di Riferimento Oncologico Aviano, Italy.
Massimo
M
Libra
Valli
V
De Re
Daniela
D
Gasparotto
Annunziata
A
Gloghini
Alessandra
A
Marzotto
Isabella
I
Milan
Umberto
U
Tirelli
Franca
F
Stivala
Antonino
A
Carbone
Mauro
M
Boiocchi
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D022801:Complementarity Determining Regions / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D005500:Follow-Up Studies; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016609:Neoplasms, Second Primary / Q000175:diagnosis* / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000473:pathology; D012008:Recurrence
D022801:Complementarity Determining Regions; D004273:DNA, Neoplasm; D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region