High-resolution magic angle spinning MRS of breast cancer tissue.
High-resolution magic angle spinning (HR MAS) may develop into a new diagnostic tool for studying intact tissue samples, and several types of cancer have been investigated with promising results. In this study HR MAS spectra of breast cancer tissue from 10 patients have been compared to conventional high-resolution spectra of perchloric acid extracts of the same tissue type. The HR MAS spectra show resolution comparable to spectra of extracts, and two-dimensional techniques lead to identification of a majority of the constituents. More than 30 different metabolites have been detected and assigned. To our knowledge this is the most detailed assignment of biochemical components in intact human breast tissue. The spectra of intact breast cancer tissue differ from perchloric acid extracts by the presence of lipids and fewer signals in the low field region. HR MAS analysis of intact breast tissue specimens is a rapid method, providing spectra with resolution where relative quantification of the majority of the detected metabolites is possible.
SINTEF Unimed, MR-Center, N-7465 Trondheim, Norway. Beathe.Sitter@unimed.sintef.no
Beathe
B
Sitter
Ursula
U
Sonnewald
Manfred
M
Spraul
Hans E
HE
Fjösne
Ingrid S
IS
Gribbestad
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000378:metabolism*; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008875:Middle Aged; D010472:Perchlorates; D011522:Protons; D014020:Tissue Extracts / Q000032:analysis
D010472:Perchlorates; D011522:Protons; D014020:Tissue Extracts