A microarray-based method for detecting methylated loci.
CpG island DNA methylation plays an important role in regulating gene expression in development and carcinogenesis. We developed a new microarray-based method called methylation amplification DNA chip (MAD) for detecting differences in methylation. In this method, only methylated CpG islands from the two samples that we wanted to compare were amplified and used for hybridization. The resource material for the microarray was derived from the methylated DNA library of the sample in which we wanted to detect hypermethylation. Choosing the methylated DNA library as the resource material of the microarray increased the percentage of DNA fragments derived from hypermethylated loci on the microarray.
Hatada
Izuho
I
Gene Research Center, Gunma University, 3-39-22 Showa-machi, Maebashi 371-8511, Japan. Ihatada@showa.gunma-u.ac.jp
Kato
Azusa
A
Morita
Sumiyo
S
Obata
Yayoi
Y
Nagaoka
Kayuri
K
Sakurada
Akira
A
Sato
Masami
M
Horii
Akira
A
Tsujimoto
Atsumi
A
Matsubara
Kenichi
K
D000818:Animals; D018899:CpG Islands; D019175:DNA Methylation; D006801:Humans; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013234:Stem Cells; D014407:Tumor Cells, Cultured