Cytochrome c folding pathway: kinetic native-state hydrogen exchange.
Native-state hydrogen exchange experiments under EX1 conditions can distinguish partially unfolded intermediates by their formation rates and identify the amide hydrogens exposed and protected in each. Results obtained define a cytochrome c intermediate seen only poorly before and place it early on the major unfolding pathway. Four distinct unfolding steps are found to be kinetically ordered in the same pathway sequence inferred before.
Hoang
Linh
L
The Johnson Research Foundation, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6059, USA. lhoang@mail.med.upenn.edu
Bedard
Sabrina
S
Krishna
Mallela M G
MM
Lin
Yan
Y
Englander
S Walter
SW
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D003574:Cytochrome c Group / Q000737:chemistry*; D006736:Horses; D006859:Hydrogen / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008958:Models, Molecular; D011489:Protein Denaturation; D017510:Protein Folding
D003574:Cytochrome c Group; D006859:Hydrogen