Nestin immunoreactivity in local neurons of the adult rat striatum after remote cortical injury.
Nestin is a marker for the neuronal and glial precursor cells and is expressed in reactive astrocytes after brain injury. Following restricted neocortical injury, we found that cells with neuronal morphology in the adult rat striatum became immunoreactive for both nestin and the neuronal marker, microtubule-associated protein 2 (MAP-2), but not for the astroglial marker, glial fibrillary acidic protein (GFAP). The number of nestin-positive cells transiently increased in the striatum. Continuous administration of 5-bromo-2'-deoxyuridine (BrdU) after cortical injury did not reveal any newly generated neurons in the striatum. Double-labeling fluorescent immunocytochemistry revealed that the nestin-positive striatal cells were also substance-P-positive. These findings suggest that some factors released from the injured cortex may induce nestin immunoreactivity in striatal neurons.
Kuroda
Tatsuya
T
Department of Neurosurgery, Gifu University School of Medicine, Japan.
Nakamura
Hiroyuki
H
Itoh
Kazuo
K
Le
Wu Ri
WR
Yoshimura
Shin-ichi
S
Takenaka
Katsunobu
K
Sakai
Noboru
N
D000818:Animals; D002540:Cerebral Cortex / Q000737:chemistry / Q000293:injuries* / Q000378:metabolism; D003342:Corpus Striatum / Q000737:chemistry / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D009419:Nerve Tissue Proteins; D064231:Nestin; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin