Prediction of protein structural class by amino acid and polypeptide composition.
A new approach of predicting structural classes of protein domain sequences is presented in this paper. Besides the amino acid composition, the composition of several dipeptides, tripeptides, tetrapeptides, pentapeptides and hexapeptides are taken into account based on the stepwise discriminant analysis. The result of jackknife test shows that this new approach can lead to higher predictive sensitivity and specificity for reduced sequence similarity datasets. Considering the dataset PDB40-B constructed by Brenner and colleagues, 75.2% protein domain sequences are correctly assigned in the jackknife test for the four structural classes: all-alpha, all-beta, alpha/beta and alpha + beta, which is improved by 19.4% in jackknife test and 25.5% in resubstitution test, in contrast with the component-coupled algorithm using amino acid composition alone (AAC approach) for the same dataset. In the cross-validation test with dataset PDB40-J constructed by Park and colleagues, more than 80% predictive accuracy is obtained. Furthermore, for the dataset constructed by Chou and Maggiona, the accuracy of 100% and 99.7% can be easily achieved, respectively, in the resubstitution test and in the jackknife test merely taking the composition of dipeptides into account. Therefore, this new method provides an effective tool to extract valuable information from protein sequences, which can be used for the systematic analysis of small or medium size protein sequences. The computer programs used in this paper are available on request.
Luo
Rui-yan
RY
Department of Mathematics, Tianjin University, Tianjin 300 072, China.
Feng
Zhi-ping
ZP
Liu
Jia-kun
JK
D000465:Algorithms; D000596:Amino Acids / Q000032:analysis / Q000737:chemistry*; D000818:Animals; D016208:Databases, Factual; D006801:Humans; D010455:Peptides / Q000737:chemistry*; D017434:Protein Structure, Tertiary
D000596:Amino Acids; D010455:Peptides