[Experimental study on growth factor in promotion of chondrocytes proliferation].
OBJECTIVE
To study the effect of two cytokines, basic fibrolast growth factor(bFGF) and insulin-like growth factor-I(IGF-I), on cell proliferation in chondrocytes of adult rabbits.


METHODS
The primary chondrocytes of adult rabbits were harvested and cultured with bFGF and IGF-I at different concentrations, respectively, as well as with the mixture of the two cytokines; the quantity of cultured chondrocytes was detected by MTT assay at the 24th, 48th and 72nd hours; and the final fold increase of different groups was measured by cell count for the 3rd passage; and the proliferation index of the groups was recorded by flowing cytometer on the 14th day.


RESULTS
1. The cultured chondrocytes with either bFGF, IGF-I or their mixture were significantly more than that of control group at the 24th, 48th and 72th hours (P < 0.01). 2. After the 3rd passage, the final folds of proliferation were significantly higher in the groups with cytokines than in the control group (P < 0.01); and the final fold with the mixture of cytokines was significantly higher than that of both IGF-I and bFGF (P < 0.01). 3. The proliferation index was significantly higher in the groups with cytokines than in the control group (P < 0.01); the proliferation index with the mixture of cytokines was significantly higher than that of both IGF-I and bFGF (P < 0.05); besides, proliferation index was higher when cytokine was applied twice than once (P < 0.05); besides, proliferation index was higher when cytokine was applied twice than once (P < 0.05).


CONCLUSIONS
bFGF and IGF-I could promote chondrocytes proliferation of adult rabbits obviously and they are synergistic in cell proliferation.
Department of Orthopedic Surgery, Second Affiliated Hospital, China Medical University, Shenyang, Liaoning, P. R. China 110004.
Qin
Q
Fu
Yi-peng
YP
Xiao
Zhi-wei
ZW
Wang
Zhong-hai
ZH
Zhao
Zhen-hai
ZH
Wang
Lei
L
Li
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects*; D004357:Drug Synergism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D011817:Rabbits
D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I