Ultrasound extraction and thin layer chromatography-flame ionization detection analysis of the lipid fraction in marine mucilage samples.
This paper reports an analytical procedure based on ultrasound to extract lipids in marine mucilage samples. The experimental conditions of the ultrasound procedure (solvent and time) were identified by a FT-IR study performed on different standard samples of lipids and of a standard humic sample, before and after the sonication treatment. This study showed that diethyl ether was a more suitable solvent than methanol for the ultrasonic extraction of lipids from environmental samples because it allowed to minimize the possible oxidative modifications of lipids due to the acoustic cavitation phenomena. The optimized conditions were applied to the extraction of total lipid amount in marine mucilage samples and TLC-flame ionization detection analysis was used to identify the relevant lipid sub-fractions present in samples.
Istituto Centrale per la Ricerca Scientifica e Tecnologica Applicata al Mare, Rome, Italy. mecoma@rdn.it
M
M
Mecozzi
M
M
Amici
G
G
Romanelli
E
E
Pietrantonio
A
A
Deluca
D002855:Chromatography, Thin Layer / Q000379:methods*; D003124:Colorimetry; D005410:Flame Ionization / Q000379:methods*; D012623:Seawater; D017550:Spectroscopy, Fourier Transform Infrared; D014465:Ultrasonics