Simultaneous identification of ryanodine receptor 1 (RYR1) and estrogen receptor (ESR) genotypes with the multiplex PCR-RFLP method in Polish Large White and Polish Landrace pigs.
A method allowing simultaneous genotyping of two loci: ryanodine receptor 1 (RYR1) and estrogen receptor (ESR) is presented. In multiplex PCR amplification, two amplicons were simultaneously produced: a 272 bp fragment of RYR1 gene and a 185 bp fragment of ESR gene and were then subjected to "one-tube" restriction enzyme digestion with Hin6 I and Ava I, respectively. A total of 122 Polish Large White and Polish Landrace pigs were genotyped by this method, demonstrating its reliability, convenience and lower costs. This method may be useful in the wide-scale genotyping of both loci in pig breeding programmes.
University of Warmia and Mazury in Olsztyn, Department of Animal Genetics, ul. Oczapowskiego 5, 10-957 Olsztyn, Poland. stachel@uwm.edu.pl
Stanisław
S
Kamiński
Anna
A
Ruść
Katarzyna
K
Wojtasik
D000818:Animals; D005838:Genotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011960:Receptors, Estrogen / Q000235:genetics*; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics*; D013552:Swine / Q000235:genetics*
D011960:Receptors, Estrogen; D019837:Ryanodine Receptor Calcium Release Channel