Determination of residues of enrofloxacin and its metabolite ciprofloxacin in chicken muscle by capillary electrophoresis using laser-induced fluorescence detection.
A method for the residue analysis of the veterinary antimicrobial agent enrofloxacin and its active desethyl metabolite ciprofloxacin in chicken muscle tissue has been developed and validated. The detection of the analytes was performed by laser-induced fluorescence (LIF) detection using a HeCd laser (lambda(ex) = 325 nm) providing an enhancement in sensitivity and selectivity compared to conventional UV detection. The assay has been validated with satisfying results. The limits of quantification for enrofloxacin and ciprofloxacin were 5 microg/kg and 20 microg/kg, respectively, with a fivefold preconcentration yielded by a sample clean-up with a simple liquid-liquid extraction procedure. Calibration graphs were linear from 5 to 1000 microg/kg for enrofloxacin and from 20 to 1000 microg/kg for ciprofloxacin. The assay allows the detection of contaminated muscle samples at the required maximum residue limit of the European Union, which is 100 microg/kg for the sum of enrofloxacin and ciprofloxacin.
Horstkötter
Christian
C
Institute of Pharmaceutical and Medicinal Chemistry, University of Münster, Münster, Germany.
Jiménez-Lozano
Eulalia
E
Barrón
Dolores
D
Barbosa
José
J
Blaschke
Gottfried
G
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis* / Q000592:standards; D002138:Calibration; D002645:Chickens; D002939:Ciprofloxacin / Q000032:analysis* / Q000592:standards; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D000077422:Enrofloxacin; D005456:Fluorescent Dyes; D024841:Fluoroquinolones; D005506:Food Contamination / Q000032:analysis; D007834:Lasers; D018482:Muscle, Skeletal / Q000737:chemistry*; D015363:Quinolones / Q000032:analysis* / Q000592:standards; D012680:Sensitivity and Specificity; D019155:Veterinary Drugs / Q000032:analysis / Q000592:standards
D000890:Anti-Infective Agents; D005456:Fluorescent Dyes; D024841:Fluoroquinolones; D015363:Quinolones; D019155:Veterinary Drugs; D000077422:Enrofloxacin; D002939:Ciprofloxacin