[Experimental study on growth factor in promotion of chondrocytes proliferation].
OBJECTIVE
To study the effect of two cytokines, basic fibrolast growth factor(bFGF) and insulin-like growth factor-I(IGF-I), on cell proliferation in chondrocytes of adult rabbits.


METHODS
The primary chondrocytes of adult rabbits were harvested and cultured with bFGF and IGF-I at different concentrations, respectively, as well as with the mixture of the two cytokines; the quantity of cultured chondrocytes was detected by MTT assay at the 24th, 48th and 72nd hours; and the final fold increase of different groups was measured by cell count for the 3rd passage; and the proliferation index of the groups was recorded by flowing cytometer on the 14th day.


RESULTS
1. The cultured chondrocytes with either bFGF, IGF-I or their mixture were significantly more than that of control group at the 24th, 48th and 72th hours (P < 0.01). 2. After the 3rd passage, the final folds of proliferation were significantly higher in the groups with cytokines than in the control group (P < 0.01); and the final fold with the mixture of cytokines was significantly higher than that of both IGF-I and bFGF (P < 0.01). 3. The proliferation index was significantly higher in the groups with cytokines than in the control group (P < 0.01); the proliferation index with the mixture of cytokines was significantly higher than that of both IGF-I and bFGF (P < 0.05); besides, proliferation index was higher when cytokine was applied twice than once (P < 0.05); besides, proliferation index was higher when cytokine was applied twice than once (P < 0.05).


CONCLUSIONS
bFGF and IGF-I could promote chondrocytes proliferation of adult rabbits obviously and they are synergistic in cell proliferation.
Fu
Qin
Q
Department of Orthopedic Surgery, Second Affiliated Hospital, China Medical University, Shenyang, Liaoning, P. R. China 110004.
Xiao
Yi-peng
YP
Wang
Zhi-wei
ZW
Zhao
Zhong-hai
ZH
Wang
Zhen-hai
ZH
Li
Lei
L
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects*; D004357:Drug Synergism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D011817:Rabbits
D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I