Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae.
The 2,160,267 bp genome sequence of Streptococcus agalactiae, the leading cause of bacterial sepsis, pneumonia, and meningitis in neonates in the U.S. and Europe, is predicted to encode 2,175 genes. Genome comparisons among S. agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, and the other completely sequenced genomes identified genes specific to the streptococci and to S. agalactiae. These in silico analyses, combined with comparative genome hybridization experiments between the sequenced serotype V strain 2603 V/R and 19 S. agalactiae strains from several serotypes using whole-genome microarrays, revealed the genetic heterogeneity among S. agalactiae strains, even of the same serotype, and provided insights into the evolution of virulence mechanisms.
Tettelin
Herve
H
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
Masignani
Vega
V
Cieslewicz
Michael J
MJ
Eisen
Jonathan A
JA
Peterson
Scott
S
Wessels
Michael R
MR
Paulsen
Ian T
IT
Nelson
Karen E
KE
Margarit
Immaculada
I
Read
Timothy D
TD
Madoff
Lawrence C
LC
Wolf
Alex M
AM
Beanan
Maureen J
MJ
Brinkac
Lauren M
LM
Daugherty
Sean C
SC
DeBoy
Robert T
RT
Durkin
A Scott
AS
Kolonay
James F
JF
Madupu
Ramana
R
Lewis
Matthew R
MR
Radune
Diana
D
Fedorova
Nadezhda B
NB
Scanlan
David
D
Khouri
Hoda
H
Mulligan
Stephanie
S
Carty
Heather A
HA
Cline
Robin T
RT
Van Aken
Susan E
SE
Gill
John
J
Scarselli
Maria
M
Mora
Marirosa
M
Iacobini
Emilia T
ET
Brettoni
Cecilia
C
Galli
Giuliano
G
Mariani
Massimo
M
Vegni
Filippo
F
Maione
Domenico
D
Rinaudo
Daniela
D
Rappuoli
Rino
R
Telford
John L
JL
Kasper
Dennis L
DL
Grandi
Guido
G
Fraser
Claire M
CM
D000595:Amino Acid Sequence; D005075:Biological Evolution; D016680:Genome, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D012703:Serotyping; D013045:Species Specificity; D013290:Streptococcal Infections / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D013296:Streptococcus pneumoniae / Q000235:genetics; D013297:Streptococcus pyogenes / Q000235:genetics; D014774:Virulence / Q000235:genetics