[The mechanism of bactericidal activity in phagosomes of neutrophils].
Myeloperoxidase plays the key role in antimicrobial of phagocytes. This enzyme uses hydrogen peroxide and chloride to catalyze hypochlorous acid formation. HOCl is the most probable agent in the oxygen-dependent bactericidal activity in the phagocyte phagosome. Chlorination markers indicate HOCl generation in the quantities lethal for bacteria. Enzymatic assay for myeloperoxidase indicates proceeding of other reactions involved in bactericidal activity. Superoxide integrates many activities of this kind and is important for physiological function of myeloperoxidase. Elucidation of phagosomes biochemistry can help us to understand why certain pathogens survive in such unfavorable environment.
Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991 Russia.
R A
RA
Murav'ev
P G
PG
But
V A
VA
Fomina
V V
VV
Rogovin
D000818:Animals; D017209:Apoptosis; D001770:Blood Bactericidal Activity / Q000502:physiology*; D006801:Humans; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry / Q000502:physiology*; D010588:Phagosomes / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism
D013481:Superoxides; D009195:Peroxidase