[A study on chemical composition of Gardenia sootepensis Hutch--quantitative determination of iridoid compounds by RP-HPLC].
OBJECTIVE
To determine the iridoid contents in Gardenia sootepensis.


METHODS
Using RP-HPLC with geniposidic acid, geniposide and scandoside methyl ether as standards.


RESULTS
According to the selected chromatographic conditions, the linear ranges of geniposidic acid, geniposide and scandoside methyl ether were 0.097-0.606 microgram, 0.0638-3.990 micrograms and 0.198-1.239 micrograms, respectively. The average recoveries of these three compounds were 100.0%, 99.9% and 100.1%, and RSD 1.23%, 1.38% and 1.53% (n = 5) respectively.


CONCLUSIONS
The method is easy, rapid, and reproducible.
National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050.
G
G
Wang
S
S
Zhao
D
D
Chen
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005638:Fruit / Q000737:chemistry; D032062:Gardenia / Q000737:chemistry* / Q000145:classification; D005960:Glucosides / Q000032:analysis*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011714:Pyrans / Q000032:analysis*
D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; C007835:geniposide; C058966:geniposidic acid