Challenges in applying microarrays to environmental studies.
Although DNA microarray technology has been used successfully to analyze global gene expression in pure cultures, it has not been rigorously tested and evaluated within the context of complex environmental samples. Adapting microarray hybridization for use in environmental studies faces several challenges associated with specificity, sensitivity and quantitation.
Zhou
Jizhong
J
Environmental Sciences Division, Oak Ridge National Laboratory, PO Box 2008, Oak Ridge, TN 37831, USA. zhouj@ornl.gov
Thompson
Dorothea K
DK
D004783:Environmental Microbiology; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000639:trends; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity