Determination of xylanase, beta-glucanase, and cellulase activity.
A simple, robust and highly reproducible method for the determination of xylanase, beta-glucanase, and cellulase in commercial feed enzyme preparations is described. The method is based on measurement of reducing moieties released by the enzymes from arabinoxylan, beta-glucan, or carboxymethylcellulose (CMC) and is independent of enzyme standards.
König
Joachim
J
Biopract GmbH, Rudower Chaussee 29, 12489 Berlin, Germany.
Grasser
Roland
R
Pikor
Heather
H
Vogel
Kurt
K
D000821:Animal Feed; D002480:Cellulase / Q000032:analysis* / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000032:analysis* / Q000378:metabolism*; D007700:Kinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013379:Substrate Specificity; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000032:analysis* / Q000378:metabolism*
D006026:Glycoside Hydrolases; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase