Oxygen distribution and consumption in rat lower incisor pulp.
The aim was to determine the oxygen tension (P(O(2))) and rate of oxygen consumption in the pulp. Twelve rats were anaesthetised and artificially ventilated. Under an operating microscope, a recessed oxygen-sensitive microelectrode was inserted into the pulp through a small saline-covered cavity on the labial surface of the lower incisor. P(O(2)) was measured as a function of the transverse distance from the saline medium through to the middle of the pulp. Oxygen profiles were characterised by a decline of oxygen tension outside the pulp in the saline medium and a steeper gradient across the interface, before a localised oxygen consuming region corresponding to the odontoblasts. A plateau with some localised fluctuations was then followed by an increase in oxygen tension in the middle of the pulp. The average oxygen tension in the plateau region was 23.2 mmHg+/-2.1 mmHg (n=12). A mathematical model was used to extract oxygen consumption data from P(O(2)) profiles recorded from non-perfused pulp (created by reducing systemic blood pressure). The analysis revealed that there was a distinct oxygen consumption zone in the outer pulp, which anatomically corresponded to the odontoblast layer. The average oxygen consumption rate of the odontoblasts was 3.2+/-0.2 ml O(2)/min per 100g pulp tissue. The zone of high oxygen consumption was 68.7 micro m+/-6.9 micro m (n=24) thick. It is concluded that pulpal oxygen distribution is heterogeneous and that the odontoblast could be a major oxygen consumer within the rat incisor pulp.
School of Dentistry, The University of Western Australia, 17 Monash Avenue, Nedlands, 6009, WA, Australia. chrisyu@cyllene.uwa.edu.au
C Y
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Yu
N M
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Boyd
S J
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Cringle
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Alder
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Yu
D000818:Animals; D003782:Dental Pulp / Q000098:blood supply* / Q000378:metabolism*; D007022:Hypotension; D007180:Incisor / Q000098:blood supply / Q000378:metabolism; D016018:Least-Squares Analysis; D008334:Mandible; D008833:Microcirculation; D008839:Microelectrodes; D008954:Models, Biological; D009804:Odontoblasts / Q000378:metabolism; D010100:Oxygen / Q000097:blood / Q000378:metabolism*; D010101:Oxygen Consumption; D010313:Partial Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D010100:Oxygen