Assignment of UVB-responsive cis-element and protoplastization-(dilution-) and elicitor-responsive ones in the promoter region of a carrot phenylalanine ammonia-lyase gene (gDcPAL1).
Expression of a carrot phenylalanine ammonia-lyase (PAL) gene (gDcPAL1) in suspension-cultured carrot cells is induced by dilution of the culture or by application of a fungal elicitor, as well as by ultraviolet B (UVB) irradiation. We demonstrated that among its upstream cis-elements (Takeda et al. [1997] Photochem. Photobiol. 66, 464-470), L4 is UVB responsive, and L1 is protoplastization- (dilution-) and elicitor responsive, from studies with transiently transformed mutated or truncated g-DcPAL1 promoter-luc constructs. This conclusion is consistent with our observation that PAL activities induced by UVB and by protoplastization (dilution) or elicitor are additive.
Takeda
Junko
J
Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan. jtakeda@kyoto.zaq.ne.jp
Ito
Yoshio
Y
Maeda
Kazuhiro
K
Ozeki
Yoshihiro
Y
D018552:Daucus carota / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D017343:Genes, Plant; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics; D018462:Photobiology; D011401:Promoter Regions, Genetic; D014466:Ultraviolet Rays
D010650:Phenylalanine Ammonia-Lyase