Assignment of UVB-responsive cis-element and protoplastization-(dilution-) and elicitor-responsive ones in the promoter region of a carrot phenylalanine ammonia-lyase gene (gDcPAL1).
Expression of a carrot phenylalanine ammonia-lyase (PAL) gene (gDcPAL1) in suspension-cultured carrot cells is induced by dilution of the culture or by application of a fungal elicitor, as well as by ultraviolet B (UVB) irradiation. We demonstrated that among its upstream cis-elements (Takeda et al. [1997] Photochem. Photobiol. 66, 464-470), L4 is UVB responsive, and L1 is protoplastization- (dilution-) and elicitor responsive, from studies with transiently transformed mutated or truncated g-DcPAL1 promoter-luc constructs. This conclusion is consistent with our observation that PAL activities induced by UVB and by protoplastization (dilution) or elicitor are additive.
Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan. jtakeda@kyoto.zaq.ne.jp
Junko
J
Takeda
Yoshio
Y
Ito
Kazuhiro
K
Maeda
Yoshihiro
Y
Ozeki
D018552:Daucus carota / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D017343:Genes, Plant; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics; D018462:Photobiology; D011401:Promoter Regions, Genetic; D014466:Ultraviolet Rays
D010650:Phenylalanine Ammonia-Lyase