Antioxidant and non-toxic properties of Piper betle leaf extract: in vitro and in vivo studies.
Piper betle leaves are used in folk medicine for the treatment of various disorders and is commonly chewed among Asians. The present study investigates the protective efficacy of P. betle leaf extract. The presence of the extract inhibited the radiation induced lipid peroxidation process effectively. This could be attributed to its ability to scavenge free radicals involved in initiation and propagation steps. Oral supplementation with extract (1, 5 and 10 mg/kg) was administered daily for 2 weeks to Swiss albino mice and the hepatic antioxidant status was analysed. The GSH content was enhanced and no appreciable change was found in the levels of oxidative damage in terms of lipid peroxidation. Also, the specific activity of SOD increased in a dose dependent manner. These factors indicate the elevation of antioxidant status in the animals. The effect on the glyoxalase system which is considered to be activated under stress conditions was also investigated. Our findings did not observe any significant change in gly I and gly II activities, implying a non-stress condition after oral treatment of the extract. The present study indicates the antioxidant activity of P. betle leaf extract and its potential to elevate the antioxidant status.
Nuclear Science Centre, Aruna Asaf Ali Marg, New Delhi, India 110067.
Dharamainder
D
Choudhary
Raosaheb K
RK
Kale
D000818:Animals; D000975:Antioxidants / Q000009:adverse effects / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays; D007791:Lactoylglutathione Lyase / Q000378:metabolism; D008297:Male; D008515:Medicine, Ayurvedic; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects; D031721:Piper betle / Q000737:chemistry*; D010936:Plant Extracts / Q000009:adverse effects / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013869:Thiolester Hydrolases / Q000378:metabolism; D000099301:Hydroxyacylglutathione Hydrolase
D000975:Antioxidants; D002374:Catalase; D007791:Lactoylglutathione Lyase; D010936:Plant Extracts; D013482:Superoxide Dismutase; D017392:Thiobarbituric Acid Reactive Substances; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase