Detection by enzyme-linked immunosorbent assay of antibodies to West Nile virus in birds.
We adapted an indirect immunoglobulin G enzyme-linked immunosorbent assay to facilitate studies of West Nile virus (WNV) and evaluated its application to taxonomically diverse avian species. Anti-WNV antibodies were detected in 23 bird species, including many exotic species, demonstrating its value in studies of WNV epizootiology.
The Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, Slingerlands, New York 12159, USA. ebel@wadsworth.org
Gregory D
GD
Ebel
Alan P
AP
Dupuis
David
D
Nicholas
Donna
D
Young
Joseph
J
Maffei
Laura D
LD
Kramer
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D001715:Bird Diseases / Q000276:immunology* / Q000821:virology*; D001717:Birds / Q000276:immunology* / Q000821:virology*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D014709:Vero Cells; D014901:West Nile Fever / Q000175:diagnosis / Q000276:immunology; D014902:West Nile virus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G