Detection of simian immunodeficiency virus in diverse species and of human immunodeficiency virus Type 2 by using consensus primers within the pol region.
Human immunodeficiency virus type 2 (HIV-2) is the result of cross-species transmission of simian immunodeficiency virus (SIV) from sooty mangabey monkeys to humans. Primer pairs (intHIV-2/SIV) based on a region of integrase that has considerable homology across HIV-2 and SIV lineages were designed to develop a broadly cross-reactive molecular assay to detect lentivirus infection in primates. The intHIV-2/SIV primers detect HIV-2 and simian viruses SIVcpz, SIVsmm, SIVsyk, SIVagm, and SIVmnd. The primers are also capable of amplifying some HIV-1 strains. Additionally, sequences from the integrase amplicons were of sufficient genetic diversity to permit not only phylogenetic clustering of all simian viruses to their respective lineages but also HIV type and group classification. Thus, the primers described here provide a method to detect primate lentiviruses from diverse species of nonhuman primates, as well as from persons infected with HIV-1 and HIV-2.
Masciotra
Silvina
S
HIV Immunology and Diagnostics Branch, Division of AIDS, Sexually Transmitted Diseases, and Tuberculosis Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Yang
Chunfu
C
Pieniazek
Danuta
D
Thomas
Chanda
C
Owen
Sherry M
SM
McClure
Harold M
HM
Lal
Renu B
RB
D000818:Animals; D016663:Cercopithecinae; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D015684:Gene Products, pol / Q000235:genetics*; D015658:HIV Infections / Q000821:virology; D019427:HIV Integrase / Q000235:genetics; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015498:HIV-2 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D016097:Simian Acquired Immunodeficiency Syndrome / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D004279:DNA, Viral; D015684:Gene Products, pol; D019427:HIV Integrase