Determination of clindamycin in animal plasma by high-performance liquid chromatography combined with electrospray ionization mass spectrometry.
A method for the quantification of clindamycin in animal plasma using high-performance liquid chromatography combined with electrospray ionization mass spectrometry (LC/ESI-MS/MS) is presented. Lincomycin is used as the internal standard. The sample preparation includes a simple deproteinization step with trichloroacetic acid. Chromatographic separation is achieved on an RP-18 Hypersil column using gradient elution with 0.01 M ammonium acetate and acetonitrile as mobile phase. Good linearity was observed in the range 0-10 microg ml(-1). The limit of quantification of the method is 50 ng ml(-1) and the limit of detection is 1.3 ng ml(-1). The method was shown out to be of use for pharmacokinetic studies of clindamycin formulations in dogs.
Cherlet
Marc
M
Faculty of Veterinary Medicine, Department of Pharmacology, Pharmacy and Toxicology, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. mcherlet@yahoo.com
Croubels
Siska
S
De Backer
Patrick
P
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002981:Clindamycin / Q000097:blood*; D004285:Dogs; D008034:Lincomycin / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
D000900:Anti-Bacterial Agents; D002981:Clindamycin; D008034:Lincomycin