Novel optical solid-state glucose sensor using immobilized glucose oxidase.
Meso-tetra(4-carboxyphenyl)porphine (CTPP(4)) binds reversibly to immobilized glucose oxidase (GOD), resulting in an absorbance peak for the CTPP(4)-GOD complex at 427nm. The absorbance intensity of the 427nm peak is reduced upon exposure to glucose, which causes the dissociation of CTPP(4) from GOD. The change in absorbance at 427nm shows linear dependence on glucose concentration from 20 to 200mg/dL (1.1-11.1mM).
Department of Physics, Oklahoma State University, 74078, Stillwater, OK, USA.
Brandy J
BJ
White
H James
HJ
James Harmon
D015374:Biosensing Techniques / Q000379:methods*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D011166:Porphyrins / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*
D011166:Porphyrins; C018395:tetracarboxyphenylporphine; D005949:Glucose Oxidase; D005947:Glucose