Corneal injury threshold in rabbits for the 1540 nm infrared laser.
BACKGROUND
In the 40 yr since lasers were invented, they have become commonplace in military operations, and while their utility in this setting is undeniable, they also represent a potential hazard for those in contact with them. This threat must be recognized, information must be gathered to understand this injury potential, and the necessary measures must be taken to properly protect those who will work, train, and fight with these systems. The exact mechanisms of laser/tissue interaction at 1540 nm are not well understood. Previous studies and textbooks show remarkable disparity in reporting where 1540 nm laser energy is deposited and the quantity of energy required to cause tissue damage. Rabbit cornea is very similar histologically to that of humans with the exception that it lacks Bowman's membrane. This model has been recommended as a reasonable approximation by past researchers and avoids the use of valuable non-human, primate research animals.


METHODS
A rabbit model was used to demonstrate the ability of the 1540 nm laser to produce corneal injuries. Various energies were applied to find the threshold at which injury is consistently produced. Observations included the appearance of the injury in the rabbit cornea. All rabbits were between 5 and 6 kg.


RESULTS
Corneal injury was consistent at energies above 56 J x cm(-2). Injuries involved the deeper corneal stroma rather than only the epithelial layer, thus raising concern for permanent visual disability in those affected. The gross appearance of these injuries was white opaque areas easily seen within the corneal stroma.


CONCLUSIONS
Data shows conclusively that the 1540 nm laser causes significant corneal damage at reproducible energy levels. Further research is clearly necessary to advance our understanding of the role of Bowman's membrane, the healing properties of the injured cornea, and the epidemiology of laser injury.
Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Thomas F
TF
Clarke
Thomas E
TE
Johnson
Margaret B
MB
Burton
Bryan
B
Ketzenberger
William P
WP
Roach
D000818:Animals; D003315:Cornea / Q000473:pathology; D065306:Corneal Injuries; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D004781:Environmental Exposure / Q000009:adverse effects; D005131:Eye Injuries / Q000209:etiology / Q000473:pathology / Q000517:prevention & control; D007259:Infrared Rays / Q000009:adverse effects*; D007834:Lasers / Q000009:adverse effects*; D016015:Logistic Models; D011817:Rabbits; D012449:Safety; D013816:Thermodynamics; D013997:Time Factors