Characterization of the complexes of furosemide with 2-hydroxypropyl-beta-cyclodextrin and sulfobutyl ether-7-beta-cyclodextrin.
The purpose of this study was to prepare and characterize complexes of furosemide with 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) and sulfobutyl ether-7-beta-cyclodextrin (SBE-7-beta-CD). Solid complexes of furosemide with 2-HP-beta-CD and SBE-7-beta-CD were prepared by using both a freeze-drying and kneading method. Physical mixtures were prepared for comparison. The inclusion complexes were characterized by differential scanning calorimetry, X-ray diffractometry (XRD) and 1H nuclear magnetic resonance spectroscopy (1H-NMR). 1H-NMR, and especially the use of the two-dimensional ROESY spectrum, was used to determine the position of the furosemide molecule inside the cyclodextrin cavity. 1H-NMR studies showed that furosemide fit into the cyclodextrin torus cavity with its furane ring nearest to the primary hydroxyl side.
Department of Pharmaceutics, School of Pharmacy, Potchefstroom University for Christian Higher Education, 2520, Potchefstroom, South Africa .
Elisma
E
Spamer
Douw G
DG
Müller
Philippus L
PL
Wessels
Johannes P
JP
Venter
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D002152:Calorimetry, Differential Scanning; D003505:Cyclodextrins / Q000737:chemistry*; D004339:Drug Compounding; D005612:Freeze Drying; D005665:Furosemide / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015394:Molecular Structure; D012995:Solubility; D014961:X-Ray Diffraction; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; C093196:SBE4-beta-cyclodextrin; D005665:Furosemide