Rapid trace analysis of alachlor in water and vegetable samples.
The use of a rapid and specific (cross-reactivity<4%) enzyme-linked immunosorbent assay (ELISA) for the determination of alachlor residues in water and vegetable samples is addressed. The analytical method consists of a fast extraction procedure followed by an optimised ELISA. The detection limit was 0.44 microg l(-1), with a linear range from 0.89 to 143.2 microg l(-1). For alachlor extraction from water samples, different solid-phase cartridges (C, Ph, C8 and C2) were assayed using MeOH as eluent. Extracts were diluted (1:4) with distilled water before ELISA. This procedure gave recoveries close to 100% with RSDs<14%. For vegetable samples, alachlor was extracted directly with MeOH and the extracts diluted 1:40 (v/v) with saline buffer prior to ELISA. The results obtained by the proposed procedure correlate well with the reference method (multiresidue extraction-GC-MS) for vegetable samples (r>0.85).
Centro Tecnológico Nacional de la Conserva (CTC), Molina de Segura, Murcia, Spain.
José Antonio
JA
Gabaldón
José Miguel
JM
Cascales
Angel
A
Maquieira
Rosa
R
Puchades
D000081:Acetamides / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006540:Herbicides / Q000032:analysis*; D012680:Sensitivity and Specificity; D014675:Vegetables / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D000081:Acetamides; D006540:Herbicides; D014874:Water Pollutants, Chemical; C000188:alachlor