Differential protein expression at the stage of neural tube closure in the mouse embryo.
Analysis of the protein complement of a biological system through proteomics provides the opportunity to directly monitor the functional readout of gene expression. In this study, proteomics was applied to the mouse embryo to investigate the molecular events underlying the processes occurring at the stage of neural tube closure. Protein profiles of embryos between embryonic days 8.5 and 10.5 exhibited a number of stage-specific changes. Identification of developmentally regulated proteins by mass spectrometry revealed several groups of functionally related proteins including circulatory, cytoskeletal, and stress proteins. Additional proteins of unknown function were identified, such as Copine 1 and PICOT, whose developmental regulation was previously unsuspected.
Neural Development Unit, Institute of Child Health, University College London, United Kingdom. n.greene@ich.ucl.ac.uk
Nicholas D E
ND
Greene
Kit-Yi
KY
Leung
Robin
R
Wait
Shajna
S
Begum
Michael J
MJ
Dunn
Andrew J
AJ
Copp
D000818:Animals; D003598:Cytoskeletal Proteins / Q000096:biosynthesis; D015180:Electrophoresis, Gel, Two-Dimensional; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D005914:Globins / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D051379:Mice; D011247:Pregnancy; D014176:Protein Biosynthesis; D020543:Proteome; D006180:Proton-Translocating ATPases / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D015854:Up-Regulation; D000509:alpha-Fetoproteins / Q000096:biosynthesis
D003598:Cytoskeletal Proteins; D006360:Heat-Shock Proteins; D020543:Proteome; D012333:RNA, Messenger; D000509:alpha-Fetoproteins; D005914:Globins; D006180:Proton-Translocating ATPases