Functional domain of bovine milk lactoferrin which inhibits the adherence of Streptococcus mutans cells to a salivary film.
The bovine lactoferrin molecule and relatively long lactoferrin fragments containing residues 473 to 538 strongly inhibited adherence of Streptococcus mutans to saliva-coated hydroxyapatite beads. Each cysteine residue in Lf411 (residues 473 to 538) was replaced by a serine residue, and the mutants Lf411-C481S and Lf411-C532S strongly inhibited S. mutans adherence. These results suggest that the functional domain of lactoferrin that binds to a salivary film lies in residues 473 to 538 and that the region might be concealed by disulfide bond formation between Cys481 and Cys532 in the Lf411 fragment.
Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan. oho@dent.kyushu-u.ac.jp
Takahiko
T
Oho
Morihide
M
Mitoma
Toshihiko
T
Koga
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002417:Cattle; D003545:Cysteine / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D017886:Durapatite; D066298:In Vitro Techniques; D007781:Lactoferrin / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D017434:Protein Structure, Tertiary; D012463:Saliva / Q000382:microbiology*; D013295:Streptococcus mutans / Q000472:pathogenicity* / Q000502:physiology*
D004269:DNA, Bacterial; D010446:Peptide Fragments; D017886:Durapatite; D007781:Lactoferrin; D003545:Cysteine