The immediate early gene of canine herpesvirus is transcribed through early and late phases.
The immediate early (IE) gene of canine herpesvirus (CHV), homologue of the infected cell protein 4 (ICP4) gene of herpes simplex virus 1, is transcribed as a 4.9kb mRNA during IE phase. The IE gene was further transcribed as a 4.8kb mRNA through early (E) and late (L) phases of productive infection. Transcription of the 4.8kb mRNA initiated from downstream of the TATA box in an intron which was spliced out during IE phase. The reverse transcription-polymerase chain reaction revealed that the IE promoter was turned off during L phase at a permissive temperature. We, thus, propose to redesignate the IE gene of CHV as CICP4 gene.
Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Masahiro
M
Miyoshi
Katsunori
K
Okazaki
Mitsuyoshi
M
Takiguchi
Hiroshi
H
Kida
Akira
A
Hashimoto
D018138:Alphaherpesvirinae / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D004285:Dogs; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics*; D017874:Immediate-Early Proteins / Q000235:genetics; D007668:Kidney; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics
D017874:Immediate-Early Proteins; D012367:RNA, Viral; D014764:Viral Proteins