Sequence-, tissue-, and delivery-specific targeting of RNA during post-transcriptional gene silencing.
Transgenic Nicotiana benthamiana plants expressing an untranslatable version of the coat protein (CP) gene from the Tamarillo mosaic virus (TaMV) were either resistant to TaMV infection or recovered from infection. These phenotypes were the result of a post-transcriptional gene silencing (PTGS) mechanism that targeted TaMV-CP sequences for degradation. The TaMV-CP sequences were degraded when present in the wild-type TaMV potyvirus, in transgene mRNA, or in chimeric viral vectors based on White clover mosaic virus. The more efficiently targeted region was mapped to a 134-nt segment. Differences were observed in the efficiency of targeting during cell-to-cell and long-distance movement of the chimeric viruses. However, the TaMV-CP sequences do not appear to be targeted for degradation when delivered by biolistics.
The Horticulture and Food Research Institute of New Zealand Limited, Auckland.
Ezequiel
E
Balmori-Melian
Robin M
RM
MacDiarmid
David L
DL
Beck
Richard C
RC
Gardner
Richard L S
RL
Forster
D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D020868:Gene Silencing; D009029:Mosaic Viruses / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics; D012323:RNA Processing, Post-Transcriptional; D012367:RNA, Viral / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*; D014779:Virus Replication
D036022:Capsid Proteins; D017931:DNA Primers; D012367:RNA, Viral