Infrared study of human serum very-low-density and low-density lipoproteins. Implication of esterified lipid C=O stretching bands for characterizing lipoproteins.
Fourier-transform infrared (FT-IR) spectroscopy was applied to examine human serum very-low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) in aqueous solution and in solid film for characterizing lipid components. On the basis of the FT-IR second-derivative spectra for standard samples of triglycerides, cholesterol esters and phospholipids, it was found that the band at 1746 cm(-1) for VLDL and the band at 1738 cm(-1) for LDL were mainly due to the unsaturated triglycerides and unsaturated cholesterol esters, respectively. The implications of ester C=O stretching bands are discussed.
Laboratory of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, 2-8-30 Kohnodai, Ichikawa, Chiba 272-0827, Japan. nara.las@tmd.ac.jp
Masayuki
M
Nara
Mitsuyo
M
Okazaki
Hiroyuki
H
Kagi
D002244:Carbon / Q000737:chemistry; D004951:Esterification; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry*; D008079:Lipoproteins, VLDL / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D002244:Carbon; D010100:Oxygen