[Effect of urotensin II on the nitric oxide production in neonatal rat cardiomyocytes].
The aim of this study was to investigate the effect of urotensin II (U II) on the nitric oxide (NO) production in cultured neonatal rat cardiomyocytes. The endothelial nitric oxide synthase (eNOS) mRNA expression was assessed by semi-quantitative reverse transcription-polymerase chain reaction. The activity of nitric oxide synthase (NOS) and NO content in cardiomyocytes were measured. The current results showed that U inhibited eNOS mRNA expression, the NOS activity and the NO production of cardiomyocytes. U II (0.1 micromol/L) inhibited the NOS activity and the NO production in cardiomyocytes in a time-dependent manner. These results suggest that the cardiovascular effect of U II might be partially associated with NO production in cultured neonatal rat cardiomyocytes.
Department of Physiology, The Second Military Medical University, Shanghai 200433.
Ling
L
Li
Wen-Jun
WJ
Yuan
Xiu-Jie
XJ
Pan
Wei-Zhong
WZ
Wang
Jing-Wei
JW
Qiu
Chao-Shu
CS
Tang
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D051381:Rats; D014579:Urotensins / Q000494:pharmacology*
D014579:Urotensins; D009569:Nitric Oxide; C029677:urotensin II; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat