Shiga toxin 2 induces macrophage-granulocyte colonies from human bone marrow and cord blood stem cells.
Addition of Shiga toxin 2 to human bone marrow or cord blood cell culture induced macrophage-granulocyte colonies. Although Shiga toxin 2 alone induced colonies mainly composed of macrophages, it induced colonies mainly consisting of granulocytes when combined with physiological doses of interleukin-1beta, granulocyte colony-stimulating factor, or stem cell factor with interleukin-3.
Nagasaki Red Cross Blood Center, Nagasaki, Japan.
Shin
S
Chiyoda
Tae
T
Takeda
Yosuke
Y
Aoki
D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D003114:Colony-Forming Units Assay; D019453:Escherichia coli O157 / Q000472:pathogenicity; D005312:Fetal Blood / Q000166:cytology / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D006098:Granulocytes / Q000187:drug effects* / Q000473:pathology; D018922:HL-60 Cells; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000473:pathology; D006463:Hemolytic-Uremic Syndrome / Q000209:etiology / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000008:administration & dosage; D007377:Interleukin-3 / Q000008:administration & dosage; D019169:Jurkat Cells; D008264:Macrophages / Q000187:drug effects* / Q000473:pathology; D022641:Shiga Toxin 2 / Q000008:administration & dosage / Q000633:toxicity*; D019089:Stem Cell Factor / Q000008:administration & dosage
D007375:Interleukin-1; D007377:Interleukin-3; D022641:Shiga Toxin 2; D019089:Stem Cell Factor; D016179:Granulocyte Colony-Stimulating Factor