[Effect of continuous gamma-radiation at low doses on clonogenic hemopoietic (CFU-S) and stromal (CFU-F) bone marrow cells ].
We studied the effects of low doses of continuous gamma-irradiation (Co60, 10 days, mean daily dose power 1.5-2.0 mGy, total dose 15 mGy) on hemopoietic and stromal progenitor cells of murine bone marrow. The content of hemopoietic clonogenic cells representing a "younger" (CFU-S-11) and more "mature" (CFU-S-7) categories in the compartment of stem cells was determined in the bone marrow. The state of bone marrow stroma was estimated by the method of in vitro cloning according to the number of progenitor cells that form colonies of fibroblasts (CFU-F) and by the method of ectopic transplantation according to the capacity of stroma of organizing and building new hemopoietic territories. Continuous gamma-irradiation at low doses, that were by one order of magnitude lower than those inducing hermesis, exerted a stimulating effect on both hemopoietic (CFU-S) and stromal (CFU-F) progenitor cells. The number of CFU-S in the compartment of stem cells of the bone marrow markedly increased and they formed larger hemopoietic territories but these cells appeared to create a qualitatively different microenvironment, which stimulated the proliferation of CFU-S.
Domaratskaia
E I
EI
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 117808 Russia. edomar@proxima.idb.ac.ru
Starostin
V I
VI
Tsetlin
V V
VV
Butorina
N N
NN
Bueverova
E I
EI
Bragina
E V
EV
Khrushchov
N G
NG
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000528:radiation effects*; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D004307:Dose-Response Relationship, Radiation; D005121:Extremities / Q000528:radiation effects; D005260:Female; D005720:Gamma Rays; D018380:Hematopoietic Stem Cell Transplantation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018561:Space Simulation; D017154:Stromal Cells / Q000528:radiation effects