Neurons possessing enzymes of dopamine synthesis in the mediobasal hypothalamus of rats. Topographic relations and axonal projections to the median eminence in ontogenesis.
We evaluated the topographic relations between tyrosine hydroxylase (TH)- and/or aromatic L-amino acid decarboxylase (AADC)-immunoreactive neurons in the arcuate nucleus (AN), as well as between TH- and/or AADC-immunoreactive axons in the median eminence (ME) in rats at the 21st embryonic day, 9th postnatal day, and in adulthood. The double-immunofluorescent technique in combination with confocal microscopy was used. Occasional bienzymatic neurons but numerous monoenzymatic TH- or AADC-immunoreactive neurons were observed in fetuses. There was almost no overlap in the distribution of monoenzymatic neurons, and therefore few appositions were observed in between. In postnatal animals, numerous bienzymatic neurons appeared in addition to monoenzymatic neurons. They were distributed throughout the AN resulting in the increased frequency of appositions. Furthermore, specialized-like contacts between monoenzymatic TH- and AADC-immunoreactive neurons appeared. The quantification of the fibers in the ME showed that there were large specific areas of the monoenzymatic TH-immunoreactive fibers and bienzymatic fibers in fetuses, followed by the gradual reduction of the former and the increase of the latter to adulthood. The specific area of the monoenzymatic AADC-immunoreactive fibers in fetuses was rather low, and thereafter increased progressively to adulthood. The fibers of all the types were in apposition in the ME at each studied age. Close topographic relations between the neurons containing individual complementary enzymes of dopamine synthesis at the level of cell bodies and axons suggest functional interaction in between.
Laboratory of Neurohistology, Institute of Normal Physiology, Russian Academy of Medical Sciences, 8 Baltiiskaya St., Moscow, Russia.
Petr V
PV
Ershov
Michael V
MV
Ugrumov
André
A
Calas
Irina G
IG
Makarenko
Monique
M
Krieger
Jean
J
Thibault
D000818:Animals; D000831:Animals, Newborn; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000096:biosynthesis; D001369:Axons / Q000201:enzymology*; D001931:Brain Mapping / Q000379:methods; D004298:Dopamine / Q000096:biosynthesis*; D004622:Embryo, Mammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D007033:Hypothalamus, Middle / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D008297:Male; D008473:Median Eminence / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D009434:Neural Pathways / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D009474:Neurons / Q000201:enzymology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis
D014446:Tyrosine 3-Monooxygenase; D001142:Aromatic-L-Amino-Acid Decarboxylases; D004298:Dopamine