Effect of NADH on the redox state of human hemoglobin.
In this work, we report that NADH can increase the autoxidation rate of hemoglobin (HbA) in a pH-dependent fashion. During this process, this cofactor is itself oxidized. The presence of superoxide dismutase (SOD) and/or catalase (CAT) can inhibit this result. At lower pH rates, the effect of NADH on the hemoglobin autoxidation rate is more enhanced; in addition, the rate of NADH oxidation is increased. Our data indicates that the reduced pyridine nucleotide may influence the redox state of human hemoglobin by a mechanism, which probably involves free radical species.
Department of Bioenergetics, Jedrzej Sniadecki University School of Physical Education Wiejska 1, 80-336 Gdansk, Poland.
Robert A
RA
Olek
Jedrzej
J
Antosiewicz
Gian Carlo
GC
Caulini
Giancarlo
G
Falcioni
D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009243:NAD / Q000378:metabolism*; D016877:Oxidants / Q000378:metabolism; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D005609:Free Radicals; D006454:Hemoglobins; D016877:Oxidants; D009243:NAD; D002374:Catalase; D013482:Superoxide Dismutase