Expression of preadipocyte factor-1(Pref-1), a delta-like protein, in healing mouse ears.
Preadipocyte factor-1 (Pref-1), a delta-like protein containing epidermal growth factor-repeats, is expressed in proliferating cells in a variety of tissues and is believed to be involved in maintaining the undifferentiated state of these cells. Using microarray analysis, reverse transcriptase-polymerase chain reaction, in-situ hybridization, and immunohistochemistry, we have identified Pref-1 expression in the healing ears of two strains of mice, MRL and C57BL/6. MRL is unusual in that ear punches completely regenerate the ear tissue along with new cartilage with no scarring. Pref-1 is more highly expressed in the MRL wounds, is uniquely found in a condensation of cells within the regenerating tissue of the blastema, and may contribute to the regenerative capacity of the MRL ear wound.
Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
Stefan Jude
SJ
Samulewicz
Alexander
A
Seitz
Lise
L
Clark
Ellen
E
Heber-Katz
D000818:Animals; D002135:Calcium-Binding Proteins; D004195:Disease Models, Animal; D004431:Ear, External / Q000293:injuries* / Q000473:pathology; D015870:Gene Expression / Q000235:genetics*; D006131:Growth Inhibitors / Q000032:analysis* / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D019463:Mice, Inbred MRL lpr; D012097:Repressor Proteins / Q000032:analysis* / Q000235:genetics*; D014945:Wound Healing / Q000235:genetics*; D014950:Wounds, Penetrating / Q000235:genetics* / Q000473:pathology
D002135:Calcium-Binding Proteins; C512925:Dlk1 protein, mouse; D006131:Growth Inhibitors; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012097:Repressor Proteins; C064045:delta protein