Type I collagen inhibits hydroxyl radical-induced apoptosis.
The extracellular matrix (ECM) plays an important role in cell differentiation and apoptosis. Collagen is the major component of ECM. Here, an ESR signal of the hydroxyl radicals (.OH) generated via Fe2+ -mediated Fenton reaction was found to be significantly inhibited by type I collagen. Further study showed that type I collagen also inhibited cell apoptosis induced by.OH, as evidenced by morphological criteria (DAPI and annexin V staining) and quantitive assays for apoptotic cells (MTT and flow-cytometric assay for subG1 cells). By addition of type I collagen in HeLa cells, the lipid peroxidation caused by.OH was inhibited and the cellular GSH was protected. In comparison with type I collagen, BSA and the denatured collagen, gelatin, lacked such antioxidative and antiapoptotic effects. Together, the results suggest that type I collagen can uniquely prevent.OH-mediated apoptosis by scavenging free radicals.
Department of Biological Sciences and Biotechnology, Tsinghua University, and State Key Laboratory of Biomembrane and Membrane Biotechnology, Beijing 100084, P.R. China.
Yuhong
Y
He
Jing
J
Chen
Jianguo
J
Ren
Guochun
G
Wang
Guoping
G
Cai
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D024042:Collagen Type I / Q000502:physiology*; D004578:Electron Spin Resonance Spectroscopy; D005978:Glutathione / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism* / Q000494:pharmacology; D015227:Lipid Peroxidation
D024042:Collagen Type I; D017665:Hydroxyl Radical; D005978:Glutathione