Pulsed amperometric detection of carbohydrates on an electrophoretic microchip.
The first report of pulsed amperometric detection (PAD) on an electrophoretic microchip is presented. A hybrid poly-(dimethylsiloxane)/glass device was coupled with a platinum working electrode for the electrochemical detection of glucose, maltose, and xylose. Under optimized detection conditions, glucose was found to respond linearly from 20 to 500 microM with a measured detection limit of 20 microM. The coupling of PAD with a microchip provides a straightforward approach to the analysis of a wide range of carbohydrates using microfluidics.
Fanguy
Joseph C
JC
Department of Chemistry, Center for Integrated Chemical Measurements, Mississippi State University, MS 39762, USA.
Henry
Charles S
CS
D002241:Carbohydrates / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D008904:Miniaturization
D002241:Carbohydrates