Pax-2 interacts with RB and reverses its repression on the promoter of Rig-1, a Robo member.
RB plays dual roles in the regulation of cell proliferation and differentiation. The nervous tissue-specific gene Rig-1, a member of the roundabout (Robo) guidance receptor family, was identified as an RB-regulated gene in the mouse embryo. Herein, we report that a 2.3kb genomic DNA fragment, which contains the first 129 bases of the 5'-untranslated region and 2.2kb of the 5'-flanking region of Rig-1, has a cell type-specific promoter activity. Rig-1 promoter activity is downregulated by RB and upregulated by Pax-2. Furthermore, Rig-1 and Pax-2 mRNAs are coexpressed in the hindbrain and spinal cord of the E11.5 mouse embryo, suggesting that Pax-2 may regulate Rig-1 expression during the embryonic stage. Pax-2 interacts with RB and reverses its transcriptional suppression on the Rig-1 promoter. In summary, the ubiquitous tumor suppressor RB and the neuron-enriched transcription factor Pax-2 may play a role in the regulation of Rig-1 expression during embryogenesis.
Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan, ROC. yuanssf@ms33.hinet.net
Shyng-Shiou F
SS
Yuan
Yao-Tsung
YT
Yeh
Eva Y H-P
EY
Lee
D016475:3T3 Cells; D020121:5' Untranslated Regions; D000818:Animals; D001921:Brain / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015536:Down-Regulation; D017930:Genes, Reporter; D015151:Immunoblotting; D017403:In Situ Hybridization; D008156:Luciferases / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism*; D051762:PAX2 Transcription Factor; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000235:genetics; D016160:Retinoblastoma Protein / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D015854:Up-Regulation; D000097905:Roundabout Proteins
D020121:5' Untranslated Regions; D004247:DNA; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D008156:Luciferases; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D051762:PAX2 Transcription Factor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D016160:Retinoblastoma Protein; D014157:Transcription Factors; D000097905:Roundabout Proteins; C497319:Pax2 protein, mouse; C118348:Robo3 protein, mouse