Liquid chromatographic determination of alcohols in food and beverages with indirect polarimetric detection using a beta-cyclodextrin mobile phase.
An indirect polarimetric detection method for the determination of alcohols has been proposed in liquid chromatography (LC). Optically active mobile-phase additives, such as beta-cyclodextrin (beta-CD), could be used to visualize optically inactive alcohols in reversed-phase LC. The visualization of alcohols is based on a perturbation of the partition of beta-CD caused by the alcohols. The detection limits of the present system at a signal-to-noise ratio of 3 were 0.031, 0.019, 0.018, 0.013, 0.011, 0.008 and 0.008% (v/v) for ethanol, 2-propanol, 1-propanol, 2-methyl-2-propanol, 2-butanol, 2-methyl-1-propanol and 1-butanol, respectively. The method was successfully applied to the determination of ethanol present in food and beverage samples.
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas University, Padang 25163, Indonesia.
Edison
E
Munaf
Rahmiana
R
Zein
Ji-Ye
JY
Jin
Toyohide
T
Takeuchi
D000438:Alcohols / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D003505:Cyclodextrins / Q000737:chemistry; D005504:Food Analysis; D007214:Indonesia; D009904:Optical Rotation; D013057:Spectrum Analysis; D047392:beta-Cyclodextrins
D000438:Alcohols; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C031215:betadex