Structural characterization of a water-soluble beta-D-glucan from fruiting bodies of Agaricus blazei Murr.
A beta-D-glucan, Ab2-2N, was isolated from the hot-water extract of fruiting bodies of Agaricus blazei Murr by ethanol precipitation, anion-exchange and gel-permeation chromatography. Its structure was investigated by composition analysis, methylation analysis, Smith degradation, mild hydrolysis, and NMR spectroscopy. It contains a (1-->6)-linked beta-D-glucopyranosyl backbone, with one side chain consisting of terminal and 3-substituted beta-D-glucopyranosyl residues attached at O-3 for every three backbone residues.
Shanghai Institute of Materia Medica, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, 294 Tai-Yuan Road, Shanghai 200031, People's Republic of China.
Qun
Q
Dong
Jian
J
Yao
Xiao-tong
XT
Yang
Ji-nian
JN
Fang
D000364:Agaricus / Q000737:chemistry* / Q000254:growth & development*; D002240:Carbohydrate Sequence; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D012995:Solubility; D014867:Water / Q000737:chemistry*
D005936:Glucans; D014867:Water; C083094:polyglucosan