Identification of a herpes B virus-specific glycoprotein d immunodominant epitope recognized by natural and foreign hosts.
The mapping of linear epitopes of B virus (Cercopithecine herpesvirus 1 and herpes B virus) glycoprotein D (gD) was accomplished by screening the constructed gD epitope library with serum from B virus-infected macaques. The immunodominant epitope, gD (362-370), was identified within the C-terminal region of B virus gD that was highly conserved among 19 B virus clinical isolates but was not present in either herpes simplex virus (HSV)-1 or HSV-2 gD. A substantial percentage of serum samples from macaques (95%) and humans (80%) infected with B virus contained antibodies to this epitope. Antibodies against HSV types 1 or 2 did not react with this epitope; thus, gD (362-370) has unique potential to detect B virus-specific antibody responses in human serum, even in the presence of antibodies to HSV-1 and HSV-2.
Department of Biology, Viral Immunology Center, Georgia State University, Atlanta 30302-4118, USA. biolmp@panther.gsu.edu
Ludmila
L
Perelygina
Holley
H
Zurkuhlen
Irina
I
Patrusheva
Julia K
JK
Hilliard
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D006566:Herpesviridae Infections / Q000175:diagnosis* / Q000662:veterinary / Q000821:virology; D006568:Herpesvirus 1, Cercopithecine / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D008251:Macaca; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000175:diagnosis / Q000821:virology; D009500:Neutralization Tests; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D016056:Immunodominant Epitopes; D010455:Peptides; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1