PCR-based identification of bacteria associated with endodontic infections.
PCR primers that target the bacterial 16S rRNA genes (or the tuf gene for the genus Enterococcus) were used to identify 10 putative bacterial pathogens in root canals with necrotic pulp. In addition, the associations of these microorganisms with symptoms and a history of diabetes mellitus were investigated. Microbial samples from the root canals of 24 teeth with necrotic pulp were included in the study. PCR with universal bacterial primers identified bacterial DNA in 22 specimens; the remaining 2 specimens were from intact teeth that had been traumatized 6 months prior to treatment. PCR with specific primers showed that preoperative symptoms were significantly associated with the presence of Streptococcus spp. (P < 0.001 by chi-square analysis). There was also a nonsignificant trend for symptoms to be associated with Fusobacterium nucleatum and Porphyromonas gingivalis (odds ratio, >2) and for diabetes mellitus to be associated with P. gingivalis and Porphyromonas endodontalis (odds ratio, >2). Cloning and sequencing of the universal PCR product in one specimen revealed the presence of an organism related to the genus Olsenella, which has not previously been described in endodontic infections.
Fouad
Ashraf F
AF
Department of Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, Connecticut 06030, U.S.A. fouad@nso.uchc.edu
Barry
Jody
J
Caimano
Melissa
M
Clawson
Michael
M
Zhu
Qiang
Q
Carver
Rachaele
R
Hazlett
Karsten
K
Radolf
Justin D
JD
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D004275:DNA, Ribosomal / Q000032:analysis; D003786:Dental Pulp Cavity / Q000382:microbiology*; D003790:Dental Pulp Necrosis / Q000382:microbiology*; D003920:Diabetes Mellitus / Q000382:microbiology; D008969:Molecular Sequence Data; D010485:Periapical Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S