Impact of laparoscopic CO2-insufflation on tumor-associated molecules in cultured colorectal cancer cells.
BACKGROUND
Laparoscopic CO2-insufflation is believed to stimulate proliferation and metastatic potential of gastrointestinal carcinomas. E-cadherin, I-CAM1, I-CAM2, and CD44 are involved into the metastatic process of different cancer cell lines. The current study investigates the influence of CO2-insufflation on the expression of E-cadherin, I-CAM1, I-CAM2, and CD44 in vitro.


METHODS
CX-2 and CC531 colon carcinoma cells (human/rat) were exposed to pneumoperitoneal CO2-insufflation. E-cadherin, I-CAM1, I-CAM2, and CD44 were measured 0, 12, 24, 48, and 72 h after CO2-insufflation using flowcytometry. Control groups were exposed to room air. Data were analyzed by the Wilcoxon-Mann-Whitney U-test.


RESULTS
Both cell lines showed significant alteration in E-cadherin, I-CAM1, and CD44 expression after CO2 exposure (p <0.05). No significant differences were found regarding I-CAM2 expression.


CONCLUSIONS
The present study demonstrates CO2-insufflation to influence the expression of E-cadherin, I-CAM1 and CD44. Whether these changes increases the metastatic potential of colorectal cancer cells in vivo needs further investigation.
Department of General and Vascular Surgery, Johann Wolfgang Goethe-University, Theodor-Stern-Kai 7, D-60590 Frankfurt/Main, Germany.
Z G
ZG
Kim
C
C
Mehl
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M
Lorenz
C N
CN
Gutt
D000230:Adenocarcinoma / Q000503:physiopathology* / Q000556:secondary; D000388:Air; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002245:Carbon Dioxide; D003110:Colonic Neoplasms / Q000503:physiopathology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D007327:Insufflation / Q000009:adverse effects*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D010535:Laparoscopy / Q000009:adverse effects*; D051381:Rats; D014407:Tumor Cells, Cultured
D015820:Cadherins; D018960:Hyaluronan Receptors; D018799:Intercellular Adhesion Molecule-1; D002245:Carbon Dioxide