Binding of Sly1 to Sed5 enhances formation of the yeast early Golgi SNARE complex.
SLY1 is an essential gene for vesicular transport between the ER and the early Golgi apparatus in Saccharomyces cerevisiae. It encodes a hydrophilic Sec1/Munc18 family protein that binds to the t-SNAREs. The amount of Sly1 protein that coprecipitated with the t-SNARE Sed5 was much reduced in a temperature-sensitive sly1(ts) mutant yeast compared with the wildtype. The mutant Sly1(ts) protein was shown to have a reduced binding activity to Sed5. In the wildtype, a detectable amount of Sly1 was found in the complex between Sed5 and the v-SNARE Bet1. In vitro formation of this complex on different membranes in yeast lysate was enhanced by the addition of recombinant Sly1. These results indicate that binding of Sly1 to Sed5 enhances trans-SNARE complex formation.
Department of Biotechnology, the University of Tokyo, Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan.
Yoichi
Y
Kosodo
Yoichi
Y
Noda
Hiroyuki
H
Adachi
Koji
K
Yoda
D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D006056:Golgi Apparatus / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051938:Munc18 Proteins; D011485:Protein Binding / Q000235:genetics; D021381:Protein Transport / Q000235:genetics; D050765:Qa-SNARE Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics; D050600:SNARE Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D022161:Transport Vesicles / Q000378:metabolism*; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D008565:Membrane Proteins; D051938:Munc18 Proteins; D050765:Qa-SNARE Proteins; D011993:Recombinant Fusion Proteins; C072520:SLY1 protein, S cerevisiae; D050600:SNARE Proteins; D029701:Saccharomyces cerevisiae Proteins; C077554:Sed5 protein, S cerevisiae; D033921:Vesicular Transport Proteins