[Enterotoxic strains of Clostridium perfringens and sporulation].
Clostridium perfringens strains of A type capable of enterotoxin (CPE) synthesis may be a potential source of food-poisoning. Suitability of methods for CPE detection on the protein level is limited by difficulties in inducing sporulation in vitro. A number of unknown facts concerning coregulation the sporulation processes and CPE synthesis are recognised. The goal of the work was to determine the level of correlation between CPE synthesis and spores formation. Enterotoxin and cpe gen were detected by RPLA after sporulation induction test and by methods based on amplification on the DNA and mRNA levels. Sixty-four C. perfringens strains of A type isolated from patients with food poisoning symptoms and from food samples were analysed. Collection of isolates was differentiated as not enterotoxic, enterotoxic, and potentially enterotoxic strains based on appropriate strain profile: plc(+), cpe(-), CPE(-); plc(+), cpe(+), CPE(+); and plc(+), cpe(-), CPE(-), respectively. No significant difference between expression of cpe mRNA in vegetative and sporulation phase was found. The obtained results indicate that sporulation is not an essential factor for cpe gene expression.
Zakład Badania Surowic i Szczepionek, PZH w Warszawie.
Ewa
E
Augustynowicz
Anna
A
Gzyl
Janusz
J
Slusarczyk
D001483:Base Sequence; D003016:Clostridium perfringens / Q000145:classification* / Q000502:physiology*; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D013045:Species Specificity; D013171:Spores, Bacterial / Q000502:physiology*
D004768:Enterotoxins; D012333:RNA, Messenger; C035598:enterotoxin, Clostridium