The X-ray crystallographic structure of Escherichia coli branching enzyme.
Branching enzyme catalyzes the formation of alpha-1,6 branch points in either glycogen or starch. We report the 2.3-A crystal structure of glycogen branching enzyme from Escherichia coli. The enzyme consists of three major domains, an NH(2)-terminal seven-stranded beta-sandwich domain, a COOH-terminal domain, and a central alpha/beta-barrel domain containing the enzyme active site. While the central domain is similar to that of all the other amylase family enzymes, branching enzyme shares the structure of all three domains only with isoamylase. Oligosaccharide binding was modeled for branching enzyme using the enzyme-oligosaccharide complex structures of various alpha-amylases and cyclodextrin glucanotransferase and residues were implicated in oligosaccharide binding. While most of the oligosaccharides modeled well in the branching enzyme structure, an approximate 50 degrees rotation between two of the glucose units was required to avoid steric clashes with Trp(298) of branching enzyme. A similar rotation was observed in the mammalian alpha-amylase structure caused by an equivalent tryptophan residue in this structure. It appears that there are two binding modes for oligosaccharides in these structures depending on the identity and location of this aromatic residue.
Department of Chemistry, Michigan State University, East Lansing 48824, USA.
Marta C
MC
Abad
Kim
K
Binderup
Jorge
J
Rios-Steiner
Raghuvir K
RK
Arni
Jack
J
Preiss
James H
JH
Geiger
D015061:1,4-alpha-Glucan Branching Enzyme / Q000737:chemistry*; D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D055672:Static Electricity; D000516:alpha-Amylases / Q000737:chemistry
D029968:Escherichia coli Proteins; D015061:1,4-alpha-Glucan Branching Enzyme; D000516:alpha-Amylases