Insertion of an exogenous domain in the adenovirus type 2 fiber globular region.
Adenoviruses have been used for gene therapy or immunization due to their ability to efficiently infect a broad range of cells and tissues. These applications as well as specificity could be improved further by redirecting binding of the virus to specific cell types. In this regard, modification of viral genes encoding coat proteins is an option to achieve improvement in retargeting. In this report, we describe a substitution in the adenovirus type 2 fiber globular region by the 44 amino acid C4 domain of human immunodeficiency virus type 1 gp120. In vitro translation analysis and immunoprecipitation assays show that the incorporation of the C4 domain into the fiber protein does not ablate its trimerization property and demonstrates the availability of the C4 epitope for interaction with monoclonal anti-C4 antibody. The recombinant adenovirus containing this modified fiber was also characterized by immunoprecipitation with the same antibody, showing the viability of such kind of modification.
Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Brazil. lzerbini@caregroup.harvard.edu
L F
LF
Zerbini
T A
TA
Libermann
A M
AM
Ventura
D000256:Adenoviridae / Q000737:chemistry / Q000378:metabolism*; D002213:Capsid / Q000737:chemistry*; D002460:Cell Line; D000939:Epitopes; D016679:Genome, Viral; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D008024:Ligands; D008954:Models, Biological; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D011485:Protein Binding; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic; D014158:Transcription, Genetic
D000939:Epitopes; D015699:HIV Envelope Protein gp120; D008024:Ligands