Gene disruption by homologous recombination in the Xylella fastidiosa citrus variegated chlorosis strain.
Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa by using the bga gene, coding for beta-galactosidase, as a model. Integration of replicative plasmids by homologous recombination between the cloned truncated copy of bga and the endogenous gene was produced by one or two crossover events leading to beta-galactosidase mutants. A promoterless chloramphenicol acetyltransferase gene was used to monitor the expression of the target gene and to select a cvaB mutant.
Fundo de Defesa da Citricultura (Fundecitrus), Araraquara, São Paulo, Brazil.
Patrice
P
Gaurivaud
Leonardo C A
LC
Souza
Andrea C D
AC
Virgílio
Anelise G
AG
Mariano
Renê R
RR
Palma
Patrícia B
PB
Monteiro
D000483:Alleles; D002701:Chloramphenicol / Q000494:pharmacology; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D020563:Gammaproteobacteria / Q000235:genetics*; D017930:Genes, Reporter; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D018741:Replication Origin / Q000235:genetics; D001616:beta-Galactosidase / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism
D002701:Chloramphenicol; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase