Biocompatibility, encrustation and biodegradation of ofloxacine and silver nitrate coated poly-L-lactic acid stents in rabbit urethra.
The purpose of this study was to evaluate the biocompatibility, encrustation and biodegradation properties of silver nitrate and ofloxacine blended caprolactone-L-lactide copolymer coated self-reinforced poly-L-lactic acid (SR-PLLA) urospirals in situ in the male rabbit urethra. SR-PLLA urospirals coated with 10% by weight silver nitrate or 5% by weight ofloxacine blended copolymer or pure copolymer were inserted into the posterior urethra of 18 male rabbits. No prophylactic antibiotics were given. The animals were sacrificed 1 or 6 months after insertion. Urethral tissue reactions were histologically scored semiquantitavely and the appearence of the stents assessed using scanning electron microscopy. The biodegradation time of SR-PLLA stents was remarkably reduced by the caprolactone coating. Silver nitrate and ofloxacine blended copolymer coated urospirals caused less tissue reaction than urospirals with a pure copolymer coating. Silver nitrate coating effectively prevented biofilm formation and stent encrustation. Silver nitrate and ofloxacine blended copolymer coated SR-PLLA urospirals had good biocompatibility properties in rabbit urethra. In particular, coating with silver nitrate may provide possibilities of preventing bacterial adhesion to bioabsorbable stents.
Department of Urology, Kuusankoski District Hospital, Kuusankoski, 45750 Sairaalamäki, Finland. am.multanen@pp.fimnet.fi
Markku
M
Multanen
Teuvo L J
TL
Tammela
Marita
M
Laurila
Jukka
J
Seppälä
Tero
T
Välimaa
Pertti
P
Törmälä
Martti
M
Talja
D000818:Animals; D000892:Anti-Infective Agents, Urinary / Q000494:pharmacology*; D001673:Biodegradation, Environmental / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000378:metabolism* / Q000494:pharmacology*; D003460:Crystallization; D008297:Male; D008855:Microscopy, Electron, Scanning; D015242:Ofloxacin / Q000494:pharmacology*; D011091:Polyesters; D011817:Rabbits; D012835:Silver Nitrate / Q000494:pharmacology*; D015607:Stents; D014521:Urethra / Q000187:drug effects* / Q000648:ultrastructure
D000892:Anti-Infective Agents, Urinary; D020099:Coated Materials, Biocompatible; D011091:Polyesters; C062968:lactide-caprolactone copolymer; D012835:Silver Nitrate; D015242:Ofloxacin