[Effects and mechanism of thrombin on the expression of tissue factor activity in cultured bovine aortic endothelial cells].
OBJECTIVE
To explore the effects and mechanism of thrombin on the expression of tissue factor (TF) activity in cultured bovine aortic endothelial cells(BAECs).


METHODS
BAECs were cultured according to Schwartz's method, and one stage clotting time assay was used to measure TF activity.


RESULTS
1. Thrombin enhanced the expression of TF activity in cultured BAECs. 2. A23187, a calcium ionophore, induced slightly the expression of TF activity, but markedly promoted the thrombin-induced TF activity in BAECs(P < 0.05). 3. TFP, a Ca2+/CaM inhibitor, inhibited the inductive effects of thrombin. 4. Pentoxifylline, a phosphodiesterase inhibitor, inhibited thrombin-induced TF activity in BAECs.


CONCLUSIONS
Thrombin can induce the expression of TF activity in cultured BAECs, and the processes of signal transduction may be associated with Ca2+/CaM-dependent pathway and cAMP.
Li
Z G
ZG
Department of Physiology, Hunan Medical University, Changsha 410078.
Wen
Z B
ZB
He
X F
XF
D000818:Animals; D001011:Aorta / Q000166:cytology; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction; D013917:Thrombin / Q000494:pharmacology*; D013925:Thromboplastin / Q000096:biosynthesis*
D002147:Calmodulin; D013925:Thromboplastin; D013917:Thrombin; D002118:Calcium