Direct electrochemistry of hemoglobin in egg-phosphatidylcholine films and its catalysis to H(2)O(2).
Direct electrochemistry of hemoglobin was observed in stable thin film composed of a natural lipid (egg-phosphatidylcholine) and hemoglobin on pyrolytic graphite (PG) electrode. Hemoglobin in lipid films shows thin layer electrochemistry behavior. The formal potential E degrees ' of hemoglobin in the lipid film was linearly varied with pH in the range from 3.5 to 7.0 with a slope of -46.4 mV pH(-1). Hemoglobin in the lipid film exhibited elegant catalytic activity for electrochemical reduction of H(2)O(2), based which a unmediated biosensor for H(2)O(2) was developed.
Laboratory of Electroanalytical Chemistry and National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People's Republic of China.
Xiaojun
X
Han
Weimin
W
Huang
Jianbo
J
Jia
Shaojun
S
Dong
Erkang
E
Wang
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon; D002384:Catalysis; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D004531:Eggs; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry*; D012680:Sensitivity and Specificity
D020099:Coated Materials, Biocompatible; D006454:Hemoglobins; D008081:Liposomes; D010713:Phosphatidylcholines; C009139:pyrolytic carbon; D002244:Carbon; D006861:Hydrogen Peroxide