The T-lineage-affiliated CD2 gene lies within an open chromatin environment in acute promyelocytic leukemia cells.
The nature of hemopoietic progenitors subject to leukemic transformation in acute myeloid leukemia (AML) has not been clearly defined. To address this issue, we have used DNase I hypersensitivity assays to study the chromatin structure surrounding the T-lineage-affiliated CD2 gene in the acute promyelocytic subtype of AML (APL). Upstream and downstream flanking regions of CD2 were found to be hypersensitive to DNase I in primary APL blasts, with an identical pattern of hypersensitive sites to those detected in cells of T-lineage. All of the sites were confirmed to be inaccessible to DNase I in B-lineage leukemia cells. The demonstration of T-cell-associated chromatin features in primary APL blasts has implications for the origin of APL that may arise in more primitive progenitors than previously considered to be the case.
Grimwade
David
D
Division of Medical and Molecular Genetics, Guy's, King's and St. Thomas' School of Medicine, London SE1 9RT, UK. david.grimwade@kcl.ac.uk
Outram
Susan V
SV
Flora
Rajinder
R
Ings
Stuart J
SJ
Pizzey
Arnold R
AR
Morilla
Ricardo
R
Craddock
Charles F
CF
Linch
David C
DC
Solomon
Ellen
E
D018801:CD2 Antigens / Q000235:genetics*; D019070:Cell Lineage; D002843:Chromatin / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D003850:Deoxyribonuclease I / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D019169:Jurkat Cells; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*
D018801:CD2 Antigens; D002843:Chromatin; D003850:Deoxyribonuclease I