Quantitative trait loci that harbor genes regulating muscle size in (MRL/MPJ x SJL/J) F(2) mice.
The genetic mechanisms that determine muscle size have not been elucidated, even though it is a key musculoskeletal parameter that reflects muscle strength. In this study, we performed a high-density genome-wide scan using 633 (MRL/MPJ x SJL/J) F(2) intercross 7-week-old mice to identify quantitative trait loci (QTL) involved in the determination of muscle size. Significant QTL were identified for muscle size and body length. Muscle size (adjusted by body length) QTL were identified on chromosomes 7, 9, 11, 14 (two QTL) and 17, which together explained 19.2% of phenotypic variance in F(2)mice, while body length QTL were located on chromosome 2 (two QTL), 9, 11 and 17 which accounted for 28.3% of phenotypic variance in F(2) mice. Three significant epistatic interactions between different QTL positions from muscle size and body length were identified ( P <0.01) on chromosomes 2, 9, 14 and 17, which explained 16.1% of the variance in F(2) mice.
Molecular Genetics Division, Musculoskeletal Disease Center, Jerry L. Pettis VA Medical Center and Loma Linda University, CA 92357, USA.
Godfred L
GL
Masinde
Xinmin
X
Li
Weikuan
W
Gu
Melanie
M
Hamilton-Ulland
Subburaman
S
Mohan
David J
DJ
Baylink
D000483:Alleles; D000704:Analysis of Variance; D000818:Animals; D001824:Body Constitution; D004843:Epistasis, Genetic; D005260:Female; D005552:Forelimb / Q000033:anatomy & histology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018895:Microsatellite Repeats; D018482:Muscle, Skeletal / Q000033:anatomy & histology* / Q000502:physiology; D010641:Phenotype; D040641:Quantitative Trait Loci