Liquid chromatographic determination of alcohols in food and beverages with indirect polarimetric detection using a beta-cyclodextrin mobile phase.
An indirect polarimetric detection method for the determination of alcohols has been proposed in liquid chromatography (LC). Optically active mobile-phase additives, such as beta-cyclodextrin (beta-CD), could be used to visualize optically inactive alcohols in reversed-phase LC. The visualization of alcohols is based on a perturbation of the partition of beta-CD caused by the alcohols. The detection limits of the present system at a signal-to-noise ratio of 3 were 0.031, 0.019, 0.018, 0.013, 0.011, 0.008 and 0.008% (v/v) for ethanol, 2-propanol, 1-propanol, 2-methyl-2-propanol, 2-butanol, 2-methyl-1-propanol and 1-butanol, respectively. The method was successfully applied to the determination of ethanol present in food and beverage samples.
Munaf
Edison
E
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas University, Padang 25163, Indonesia.
Zein
Rahmiana
R
Jin
Ji-Ye
JY
Takeuchi
Toyohide
T
D000438:Alcohols / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D003505:Cyclodextrins / Q000737:chemistry; D005504:Food Analysis; D007214:Indonesia; D009904:Optical Rotation; D013057:Spectrum Analysis; D047392:beta-Cyclodextrins
D000438:Alcohols; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C031215:betadex