Expression of a linker histone-like gene in the primordial germ cells in zebrafish.
Similar to many other organisms, specification of primordial germ cells (PGCs) in zebrafish occurs during early development and depends on inheritance of 'germ plasm' determinants. Following their specification, the PGCs exhibit characteristic transcriptional profile and cell behaviour. Here we describe the cloning, expression pattern and sub-cellular localization of the zebrafish H1 type linker histone, H1M, which is specifically expressed in the PGCs in the second phase of their development. H1M transcripts are uniformly distributed during blastula stages, but become restricted to the PGCs during gastrulation and remain visible in PGCs as they migrate towards the region of the gonad.
Germ Cell Development, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
Katja
K
Müller
Christine
C
Thisse
Bernard
B
Thisse
Erez
E
Raz
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000378:metabolism*; D006657:Histones / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics*
D006657:Histones; D012333:RNA, Messenger; D029961:Zebrafish Proteins