Genetic characterization of dengue virus type 3 isolates in the State of Rio de Janeiro, 2001.
The genetic characterization of dengue virus type 3 (DEN-3) strains isolated from autochthonous cases in the State of Rio de Janeiro, Brazil, in 2001 is presented. Restriction site-specific (RSS)-PCR performed on 22 strains classified the Brazilian DEN-3 viruses as subtype C, a subtype that contains viruses from Sri Lanka, India, Africa and recent isolates from Central America. Nucleic acid sequencing (positions 278 to 2550) of one DEN-3 strain confirmed the origin of these strains, since genotype III - classified by sequencing - and RSS-PCR subtype C are correlated. This genetic subtype has been associated with hemorrhagic dengue epidemics and the information provided here could be useful to implement appropriate prevention and control measures.
Miagostovich
M P
MP
Departamento de Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil. marizepm@ioc.fiocruz.br
dos Santos
F B
FB
de Simone
T S
TS
Costa
E V
EV
Filippis
A M B
AM
Schatzmayr
H G
HG
Nogueira
R M R
RM
D001938:Brazil; D030561:Databases, Nucleic Acid; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping