[Effects of conditioned medium derived from different keratinocytes on proliferation and collagen synthesis of hypertrophic scar fibroblasts].
OBJECTIVE
To observe the effects of supernatants of normal skin keratinocytes(NK) and scar keratinocytes(SK) on proliferation and collagen synthesis of hypertrophic scar fibroblasts(HSFB).


METHODS
The supernatant, collected from cultured NK and SK, was added to the cultivated HSFB. The MTT-method, 3H-proline incorporation and radioimmunoassay were employed to measure the cell proliferation, collagen synthesis and secretion.


RESULTS
NK supernatant could inhibit HSFB proliferation and increase the collagen synthesis, but inhibit collagen secretion, as compared with the control group. On the contrary, SK supernatant could increase collagen synthesis and secretion, which had little effects on HSFB proliferation.


CONCLUSIONS
Keratinocytes derived from normal skin and hypertrophic scar show different effects on hypertrophic scar fibroblasts.
Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Shuzhong
S
Guo
Linxi
L
Zhang
Zhen
Z
Wang
Jianbo
J
Liu
D002455:Cell Division; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000096:biosynthesis*; D017077:Culture Media, Conditioned; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000502:physiology*
D017077:Culture Media, Conditioned; D003094:Collagen