A RP-LC method with evaporative light scattering detection for the assay of simethicone in pharmaceutical formulations.
A reversed-phase liquid chromatographic method has been developed and validated for the determination of the polydimethylsiloxane (PDMS) component of Simethicone, which is used as an anti-foaming agent in pharmaceutical formulations. The method involves acidification to neutralise antacid components of the formulation, then a single extraction of the PDMS with dichloromethane. This is followed by separation with a reversed-phase column using an acetonitrile-chloroform solvent gradient, and quantification by an evaporative light scattering detector. An assay precision of 3% was achieved in intraday and interday determinations. No interference was found from the aluminium and magnesium hydroxide components of antacid formulations.
Faculty of Pharmacy, The University of Sydney, Sydney 2006, Australia. demoore@pharm.usyd.edu.au
Douglas E
DE
Moore
Tina X
TX
Liu
William G
WG
Miao
Alison
A
Edwards
Russell
R
Elliss
D000536:Aluminum Hydroxide / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D008027:Light; D008276:Magnesium Hydroxide / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis; D012542:Scattering, Radiation; D012841:Simethicone / Q000032:analysis*
D004338:Drug Combinations; D004364:Pharmaceutical Preparations; C008570:aluminum hydroxide, magnesium hydroxide, simethicone drug combination; D000536:Aluminum Hydroxide; D012841:Simethicone; D008276:Magnesium Hydroxide