Peripheral blood lymphocyte subsets in adolescents: a longitudinal analysis from the REACH project.
Flow cytometry analysis of lymphocyte subset markers was performed for a group of sexually active, human immunodeficiency virus (HIV)-negative adolescents over a 2-year period to establish normative data. Data were collected in the REACH Project (Reaching for Excellence in Adolescent Care and Health), a multicenter, longitudinal study of HIV-positive and high-risk HIV-negative adolescents. Two- and three-color flow cytometry data were collected every 6 months for these subjects. We determined the effects of gender, race, and age on the following lymphocyte subset markers: total CD4(+) cells, CD4(+) naïve cells, CD4(+) memory cells, all CD8(+) cells, CD8(+) naïve cells, CD8(+) memory cells, CD16(+) natural killer cells, and CD19(+) B cells. Gender was the demographic characteristic most frequently associated with differences in lymphocyte subset measures. Females had higher total CD4(+) cell and CD4(+) memory cells counts and lower CD16(+) cell counts than males. Age was associated with higher CD4(+) memory cell counts as well as higher CD8(+) memory cell counts. For CD19(+) cells, there was an interaction between age and gender, with males having significantly lower CD19(+) cell counts with increasing age, whereas there was no age effect for females. Race and/or ethnicity was associated with differences in total CD8(+) cell counts and CD8(+) memory cell counts, although both of these associations involved an interaction with gender.
Children's Hospital of Philadelphia, Department of Pediatrics, The University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, 19104, USA. rudy@email.chop.edu
Bret J
BJ
Rudy
Craig M
CM
Wilson
Stephen
S
Durako
Anna-Barbara
AB
Moscicki
Larry
L
Muenz
Steven D
SD
Douglas
D000293:Adolescent; D000328:Adult; D018941:Antigens, CD19 / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000166:cytology*; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000166:cytology*; D002648:Child; D015331:Cohort Studies; D005260:Female; D005434:Flow Cytometry / Q000592:standards*; D006801:Humans; D007156:Immunologic Memory; D008137:Longitudinal Studies; D008297:Male; D017452:Receptors, IgG / Q000032:analysis; D012016:Reference Values
D018941:Antigens, CD19; D017452:Receptors, IgG