A novel low molecular weight hemorrhagic toxin from Trimeresurus flavoviridis (Vorojima) venom.
A low molecular weight hemorrhagic toxin, LMHT, was isolated from the venom of Trimeresurus flavoviridis from Yorojima using Q-Sepharose column chromatography. The purified hemorrhagic toxin was homogeneous by disc polyacrylamide gel electrophoresis at pH 8.3, and Ouchterlony immunodiffusion. LMHT has a molecular weight of 16,500 and possesses hemorrhagic activity. It did not show casein, azocasein, azoalbumin, or TAME (tosyl-L-arginine methyl ester hydrochloride) hydrolytic activities. Hemorrhagic activity was inhibited by EDTA, TEP (tetraethylenepentamine), p-APMSF(p-amidinophenylmethanesulfonylfluoride HCl), and N-bromosuccinimide. The minimum hemorrhagic dose of this hemorrhagic toxin was 7.1 microg/mouse. LMHT induced hydrolysis of the Aa and Bbeta chains of bovine fibrinogen.
Nikai
Toshiaki
T
Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan. nikai@ccmfs.meijo-u.ac.jp
Tateoka
Maki
M
Komori
Yumiko
Y
Tatematsu
Rieko
R
D000818:Animals; D002845:Chromatography; D003435:Crotalid Venoms / Q000009:adverse effects* / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006470:Hemorrhage / Q000139:chemically induced*; D051379:Mice; D008970:Molecular Weight; D011506:Proteins / Q000378:metabolism
D003435:Crotalid Venoms; D011506:Proteins; C029748:Trimeresurus venoms