Transformation of cinoxacin by Beauveria bassiana.
The ability of the fungus Beauveria bassiana ATCC 7159 to transform the antibacterial agent cinoxacin was investigated. Cultures in sucrose-peptone broth were dosed with cinoxacin, grown for 20 days, and then extracted with ethyl acetate. Two metabolites were detected and purified by high-performance liquid chromatography. The major metabolite was identified by mass and proton nuclear magnetic resonance spectra as 1-ethyl-1,4-dihydro-3-(hydroxymethyl)[1,3]dioxolo[4,5-g]cinnolin-4-one and the minor metabolite was identified as 1-ethyl-1,4-dihydro-6,7-dihydroxy-3-(hydroxymethyl)cinnolin-4-one. B. bassiana also reduced quinoline-3-carboxylic acid to 3-(hydroxymethyl)quinoline.
National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079-9502, USA.
Igor A
IA
Parshikov
Joanna D
JD
Moody
Thomas M
TM
Heinze
James P
JP
Freeman
Anna J
AJ
Williams
John B
JB
Sutherland
D042462:4-Quinolones; D000890:Anti-Infective Agents / Q000378:metabolism*; D001203:Ascomycota / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D002937:Cinoxacin / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy
D042462:4-Quinolones; D000890:Anti-Infective Agents; D002937:Cinoxacin