Micronuclei frequencies in hospital workers occupationally exposed to low levels of ionizing radiation: influence of smoking status and other factors.
In the context of a medical surveillance program aimed at preventing cancer risk from exposure to ionizing radiation, we investigated chromosomal damage in peripheral lymphocytes from 37 hospital workers exposed to low levels of ionizing radiation and 37 controls. The micronuleus (MN) assay was used as a biomarker of genetic damage. The influence of confounding factors like smoking status, age and gender was investigated by multiple regression analysis. The results indicated that, overall, MN frequency was higher in exposed workers than in controls, although the difference was not statistically significant. Interestingly, smoking status significantly raised MN frequency among the exposed workers but not among controls. This suggests that smoking can influence chromosomal damage induced in humans by ionizing radiation. Among both exposed workers and controls, MN frequency was found to increase with age. Female gender influenced the increase in MN frequency in the exposed group. Our results suggest that the effect of cigarette smoking should be carefully factored into genetic monitoring studies assessing the risks associated with low level radiation exposure.
Department of Pharmacology, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy. fmaffei@biofarm.unibo.it
Francesca
F
Maffei
Sabrina
S
Angelini
Giorgio Cantelli
GC
Forti
Vittorio
V
Lodi
Francesco Saverio
FS
Violante
Stefano
S
Mattioli
Patrizia
P
Hrelia
D000328:Adult; D017677:Age Distribution; D016022:Case-Control Studies; D002869:Chromosome Aberrations / Q000528:radiation effects*; D005260:Female; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects*; D016273:Occupational Exposure / Q000032:analysis*; D010564:Personnel, Hospital; D011839:Radiation, Ionizing; D017678:Sex Distribution; D012907:Smoking / Q000009:adverse effects*; D014965:X-Rays