The strength of the OVA-induced airway inflammation in rats is strain dependent.
To examine the influence of genetics on the OVA-induced allergic inflammatory response in lungs we compared rats that are genetically Th2-predisposed (Brown Norway, inbred) or not genetically predisposed (Sprague Dawley, outbred). Rats were sensitized with ovalbumin (OVA) and challenged four weeks later with OVA aerosol. Eighteen hours after challenge, lung tissue was studied for evaluation of numbers of eosinophils, neutrophils, macrophages and mast cells, as well as for expression of P-selectin, E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on endothelial cells. From a separate portion of the pulmonary tissue, leucocytes were isolated to analyse numbers of IFNgamma and IL-4 producing cells (ELISPOT assay) and frequencies of T-cell subsets and B cells. We found increased numbers of eosinophils and neutrophils in the lung, an increased number of IL-4 producing cells in lung cell isolates and increased levels of serum (OVA- specific)-IgE in both rat strains. In addition, expression of E-selectin and ICAM-1 was up regulated in both rat strains whereas expression of VCAM-1 was only up regulated in the BN rat. Although the 'allergic' Th2 response to OVA was detectable in both rat strains, it was more pronounced in the BN rat than in the SD rat. However, the SD rat, which is not predisposed to respond in either a Th2 or Th1-like way, appeared capable of mounting an allergic response to OVA. This suggests that other factors than genetic contribute to allergic disease.
Department of Pathology and Laboratory Medicine, University Hospital Groningen, The Netherlands.
M N
MN
Hylkema
M O
MO
Hoekstra
M
M
Luinge
W
W
Timens
D000818:Animals; D001249:Asthma / Q000235:genetics / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D020022:Genetic Predisposition to Disease; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation / Q000235:genetics / Q000276:immunology; D007700:Kinetics; D007962:Leukocytes / Q000276:immunology; D008168:Lung / Q000166:cytology / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000145:classification / Q000276:immunology; D008297:Male; D010047:Ovalbumin / Q000276:immunology*; D011657:Pulmonary Eosinophilia / Q000276:immunology; D051381:Rats; D011914:Rats, Inbred BN; D017207:Rats, Sprague-Dawley; D013045:Species Specificity
D015815:Cell Adhesion Molecules; D016207:Cytokines; D007073:Immunoglobulin E; D010047:Ovalbumin