[Cloning and characterization of cuticle degrading enzyme CDEP-1 from Beauveria bassiana].
Beauveria bassiana extracellular subtilisin-like serine endoprotease is a potential virulence factor by virtue of its activity against insect cuticles. A cDNA library was constructed using mRNA from mycelia of Beauveria bassiana grown on cuticle/chitin cultures. A cDNA clone of the protease, designated CDEP-1, was isolated from cDNA library. CDEP-1 contained an 1,134 bp ORF that predicted a protein of 377 amino acids with M(r) = 38,616 and PI = 8.302. The amino acid sequence of the gene shows 57.9%, 83.3% and 54.7% identity to Metarhizium nisopliae Pr1, Beauveria bassiana Pr1 and proteinase K, respectively. Southern analysis indicated that CDEP-1 was present as singly copy in Beauveria bassiana.
Fang
Wei-Guo
WG
Biotechnology Research Center Southwest Agricultural University, Beibei Chongqing 400716, China.
Zhang
Yong-Jun
YJ
Yang
Xing-Yong
XY
Wang
Zhong-Kang
ZK
Pei
Yan
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D019476:Insect Proteins / Q000378:metabolism*; D003904:Mitosporic Fungi / Q000201:enzymology*; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics*
D005656:Fungal Proteins; D019476:Insect Proteins; C047663:cuticle proteins, insects; D012697:Serine Endopeptidases