[DNA flow cytometry of bone metastases of different primary tumors].
OBJECTIVE
Some human tumors of different primary localization metastasize preferably into the bone. This concerns especially kidney, breast, prostate and lung cancer. So far, the reason for this specific pattern of metastazing could not sufficiently be clarified. The aim of the study was the search for mutuality in the results of the DNA analysis of bone metastases of different primary tumors and their importance for prognosis and therapy.


METHODS
In this study, the DNA content of 40 excised bone metastases of different primary localisation has been determined by flow cytometry. By means of the DNA distribution pattern, the percentage of tumor cells in the particular cell cycle phase as well as the respective ploidy condition have been calculated.


RESULTS
Altogether, no preference of DNA diploid or DNA aneuploid stem cell lines could be found. Whether this can be applied for metastases of all primary tumors has not been clarified yet. The fact that, in 11 out of 12 tested cases of the same metastasis, DNA diploid as well as DNA aneuploid areas could be demonstrated, seems to be fundamental.


CONCLUSIONS
There exist a high variability of the extent of dysregulation and the intensity of the growth of bone metastasis. This does not seem to be crucial for the preferable tendency to metastazise into the bone. Due to the often insufficient tumor amount for material extraction from different areas and the large amount of DNA diploid metastases after flow cytometry analysis there should be considered the additional use of pictorial analytical methods for the DNA ploidy and proliferation assessment. A knowledge of the intensity of the course and the degree of dysregulation of tumor cell proliferation of bone metastases is beneficial in order to estimate the prognosis and to design an individual therapy regime.
Klinik und Poliklinik für Orthopädie und Physikalische Medizin, Martin-Luther-Universität Halle-Wittenberg. david.wohlrab@medizin.uni-halle.de
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Reichel
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D000328:Adult; D000368:Aged; D001859:Bone Neoplasms / Q000473:pathology / Q000556:secondary* / Q000601:surgery; D001842:Bone and Bones / Q000473:pathology / Q000601:surgery; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D011379:Prognosis
D004273:DNA, Neoplasm