[Effect of exogenous hyaluronan on wound healing].
OBJECTIVE
To investigate the mechanism of impeded wound healing by exogenous hyaluronan (HA).


METHODS
Wound healing models were established on 18 adult rabbit ears, which were randomly divided into 3 groups, the 2% HA treated-group (group A), the 1% HA treated-group(group B), and the PBS control group (group C). The process of wound healing was observed morphologically and histologically. The expression of alpha-smooth muscle actin in fibroblast was measured by immunohistochemical method.


RESULTS
1. The mean values of wound healing time of groups A, B and C were (11.7 +/- 0.6), (11.3 +/- 0.6), and (10.8 +/- 1.0) days respectively. Wound contraction was greater in group C than in group A and group B. 2. Compared with PBS controls, the collagen fibril was slender and arrayed regularly in HA treated wound. 3. The expression of alpha-smooth muscle actin was greater in group C than in groups A and B.


CONCLUSIONS
It is one of reasons of impeded wound healing that exogenous HA inhibits the expression of alpha-smooth muscle protein and wound contraction. There exists dose-dependent effect.
Liu
Da-lie
DL
Department of Plastic Surgery, Shenyang Army General Hospital, Shenyang, Liaoning, P. R. China 110016.
Li
Xi-jun
XJ
Zhang
Yang
Y
Li
Yu-xia
YX
D000199:Actins / Q000096:biosynthesis; D000818:Animals; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D000199:Actins; D006820:Hyaluronic Acid