[Effect of ethanol on ATP-hydrolase activity of myometrial actomyosin].
In order of estimating some regularities of ethanol effective action on the uterus smooth muscles contractile proteins the effects of spiritus introduced into incubation medium on myometrium actomyosine ATP-hydrolase activity and superprecipitation was studied. ATP-hydrolase activity was displayed as more sensitive to ethanol action; its dependence on ethyl spiritus concentration had three-phase character expressed in two inhibiting and one activating sites. While defining the kinetic parameters of ATP hydrolysis reaction catalysed by uterus myometrium the correlation between inhibition the ATP-hydrolase activity of myometrium contractile complex under introducing into incubation medium 2% ethanol and decreasing the affinity of actomyosine to Mg2+ was made; the highest activating effect of ethanol on ATP-hydrolase activity of actomyosine complex in the presence of 8% ethanol correlated with increasing the affinity of actomyosine to Mg2+ and ATP.
Labyntseva
R D
RD
Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv.
Ul'ianenko
T V
TV
Kosterin
S O
SO
D000205:Actomyosin / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D009215:Myometrium / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D013552:Swine
D000431:Ethanol; D000255:Adenosine Triphosphate; D000205:Actomyosin; D006867:Hydrolases