Antibody responses of cattle with respiratory coronavirus infections during pathogenesis of shipping fever pneumonia are lower with antigens of enteric strains than with those of a respiratory strain.
The serum antibody responses of cattle with respiratory coronavirus infections during the pathogenesis of shipping fever pneumonia were analyzed with different bovine coronavirus antigens, including those from a wild-type respiratory bovine coronavirus (RBCV) strain (97TXSF-Lu 15-2) directly isolated from lung tissue from a fatally infected bovine, a wild-type enteropathogenic bovine coronavirus (EBCV) strain (Ly 138-3), and the highly cell culture-adapted, enteric prototype strain (EBCV L9-81). Infectivity-neutralizing (IN) and hemagglutinin-inhibiting (HAI) activities were tested. Sequential serum samples, collected during the onset of the respiratory coronavirus infection and at weekly intervals for 5 weeks thereafter, had significantly higher IN and HAI titers for antigens of RBCV strain 97TXSF-Lu15-2 than for the wild-type and the highly cell culture-adapted EBCV strains, with P values ranging from <0.0001 to 0.0483. The IN and HAI antibody responses against the two EBCV strains did not differ significantly, but the lowest titers were detected with EBCV strain L9-81.
Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
Xiao-Qing
XQ
Lin
Kathy L
KL
O'Reilly
Johannes
J
Storz
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000097:blood; D002417:Cattle; D018352:Coronavirus Infections / Q000276:immunology* / Q000662:veterinary*; D017938:Coronavirus, Bovine / Q000145:classification / Q000276:immunology*; D007422:Intestines / Q000821:virology; D008168:Lung / Q000821:virology; D009500:Neutralization Tests; D012766:Pasteurellosis, Pneumonic / Q000209:etiology / Q000276:immunology*; D012703:Serotyping / Q000662:veterinary
D000914:Antibodies, Viral; D000956:Antigens, Viral