Simple and reliable method for detection and genotyping of hepatitis C virus RNA in dried blood spots stored at room temperature.
We describe a simple, sensitive, and reproducible method for using whole blood collected onto filter paper (dried blood spots) for detection and genotyping of hepatitis C virus RNA that can be useful in large field studies, particularly in settings where collection, preparation, storage, and shipment of samples at controlled temperature can be difficult.
Solmone
Mariacarmela
M
National Institute for Infectious Diseases L. Spallanzani, IRCCS, Rome, Italy.
Girardi
Enrico
E
Costa
Francesco
F
Pucillo
Leopoldo
L
Ippolito
Giuseppe
G
Capobianchi
Maria R
MR
D001800:Blood Specimen Collection / Q000379:methods*; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000821:virology; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D015203:Reproducibility of Results; D013696:Temperature
D012367:RNA, Viral