Genomic organization and fine-mapping of the human leucine zipper-bearing kinase (LZK) gene.
Leucine zipper-bearing kinase (LZK) is a new member of the mixed lineage protein kinase family. We previously cloned a cDNA encoding LZK from a human cerebellum cDNA library. The following studies indicated that LZK serves as a MAPKKK in the JNK/SAPK pathway in cells, and a scaffold protein, JIP-1, enhances LZK-induced JNK/SAPK pathway activation via physical association. Here we report characterization of the gene structure and fine chromosomal mapping of the human LZK gene. Polymerase chain-reaction (PCR) studies indicated that the human LZK coding sequence is composed of 13 exons, and that all the splice acceptor and donor sequences obey the GT-AG rule. Chromosomal localization studies involving FISH mapping demonstrated that the human LZK gene is located at 3q27.
Ikeda
Atsushi
A
Department of Biological Chemistry and CREST (Core Research for Educational Science and Technology) Project, Japan Science and Technology Corporation, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
Sakuma
Hiroyuki
H
Masaki
Megumi
M
Kozutsumi
Yasunori
Y
Oka
Shogo
S
Inazawa
Johji
J
Kawasaki
Toshisuke
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D016350:Leucine Zippers / Q000235:genetics; D020930:MAP Kinase Kinase Kinases / Q000235:genetics*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D018076:DNA, Complementary; D020930:MAP Kinase Kinase Kinases; C109162:MAP3K13 protein, human