Mechanism of rapid transcriptional induction of tumor necrosis factor alpha-responsive genes by NF-kappaB.
NF-kappaB induces the expression of genes involved in immune response, apoptosis, inflammation, and the cell cycle. Certain NF-kappaB-responsive genes are activated rapidly after the cell is stimulated by cytokines and other extracellular signals. However, the mechanism by which these genes are activated is not entirely understood. Here we report that even though NF-kappaB interacts directly with TAF(II)s, induction of NF-kappaB by tumor necrosis factor alpha (TNF-alpha) does not enhance TFIID recruitment and preinitiation complex formation on some NF-kappaB-responsive promoters. These promoters are bound by the transcription apparatus prior to TNF-alpha stimulus. Using the immediate-early TNF-alpha-responsive gene A20 as a prototype promoter, we found that the constitutive association of the general transcription apparatus is mediated by Sp1 and that this is crucial for rapid transcriptional induction by NF-kappaB. In vitro transcription assays confirmed that NF-kappaB plays a postinitiation role since it enhances the transcription reinitiation rate whereas Sp1 is required for the initiation step. Thus, the consecutive effects of Sp1 and NF-kappaB on the transcription process underlie the mechanism of their synergy and allow rapid transcriptional induction in response to cytokines.
Ainbinder
Elena
E
Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.
Revach
Merav
M
Wolstein
Orit
O
Moshonov
Sandra
S
Diamant
Noam
N
Dikstein
Rivka
R
D002843:Chromatin / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019169:Jurkat Cells; D008954:Models, Biological; D008957:Models, Genetic; D016328:NF-kappa B / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D016329:Sp1 Transcription Factor / Q000378:metabolism; D013997:Time Factors; D035362:Transcription Factor TFIID; D019473:Transcription Factors, TFII / Q000378:metabolism*; D014158:Transcription, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D002843:Chromatin; D016328:NF-kappa B; D016329:Sp1 Transcription Factor; D035362:Transcription Factor TFIID; D019473:Transcription Factors, TFII; D014409:Tumor Necrosis Factor-alpha