Proton dipolar recoupling in resin-bound peptides under high-resolution magic angle spinning.
Rotational resonance and radiofrequency-driven dipolar recoupling (RFDR) experiments have been used to recover the weak proton dipolar interaction present in peptides bound to swollen resins spun at the magic angle. The intensity of the correlation peaks obtained using these sequences is shown to be significantly stronger than the one obtained using the classical NOESY experiment. In addition, it is found that during the relatively long mixing times required to transfer magnetization in such soft materials, the RFDR sequence also achieves magnetization transfer via the scalar J-coupling.
Institut de Chimie, UMR 7510 CNRS-Bruker, Université Louis Pasteur, Strasbourg, France.
Jésus
J
Raya
Alberto
A
Bianco
Julien
J
Furrer
Jean-Paul
JP
Briand
Martial
M
Piotto
Karim
K
Elbayed
D000595:Amino Acid Sequence; D029544:Foot-and-Mouth Disease Virus / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D012116:Resins, Plant / Q000737:chemistry*
D010455:Peptides; D012116:Resins, Plant