[Complete genome sequence of 84FLi, a Hantaan virus strain isolated from the liver of fetus aborted by a pregnant women with hemmorrhagic fever with renal syndrome].
OBJECTIVE
To study the complete genome sequence of the Chinese Hantaan virus vaccine strain 84FLi and learn about its molecular characters.


METHODS
The virus strain 84FLi was isolated from the liver of a fetus aborted by pregnant women with hemorrhagic fever with renal syndrome. The cDNAs of L  M and S segments were amplified fragment by fragment using RT-PCR. The purified PCR products were sequenced directly or cloned into pMD18-T vector and then sequenced.


RESULTS
The complete genome of strain 84FLi was composed of L ( 6 533 bp)   M (3 616 bp) and S (1 688 bp)  coding 2151  1135 and 429 amino acids  respectively. The entire sequence composition was 3830A  2050C  2510G and 3447T  the GC and AT contents were 38.52% and 61.48%. Homology analysis showed that the homologies of 84 FLi S segment nucleotide sequences with strain RG9 (isolated in Guangzhou) and strain Chen4 (isolated in Anhui) were 99.6%. There were 83.7%  84.0% and 87.2% nucleotide sequences homology with the three segments of Hantaan virus foreign standard strain  76-118   while the amino acids sequences homology with those of 76-118 were 97.5%  96.0% and 97.9% respectively.


CONCLUSIONS
The strain 84FLi is highly related to other Chinese Hantaan virus isolates and is in the same subtype with the other two Chinese isolates RG9 and Chen4.
Center for Infectious Diseases of People's Liberation Army  Tangdu Hospital  Fourth Military Medical University   Xi'an 710038, China.
Xinhong
X
Li
Weisong
W
Yang
Changshou
C
Hang
Guangyu
G
Li
Jiuping
J
Wang
D000595:Amino Acid Sequence; D001483:Base Sequence; D005260:Female; D005333:Fetus / Q000821:virology*; D016679:Genome, Viral; D018063:Hantaan virus / Q000235:genetics*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000821:virology*; D006801:Humans; D008099:Liver / Q000821:virology*; D010802:Phylogeny; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D017385:Sequence Homology