Decreased inhibitory activity of PKC in OCD patients after six months of treatment.
We investigated 5-HT reuptake and protein kinase of type C (PKC) activation in platelets of 14 OCD patients at baseline and after six months of treatment with different serotonin (5-HT) reuptake inhibitors (SRIs). The results showed that all SRIs provoked a significant increase in both the maximal velocity (V(max)) and the Michaelis-Menten constant (K(m)) of 5-HT reuptake, as compared with baseline values. The activation of PKC by means of 4-beta-12-tetradecanoylphorbol 13-acetate provoked a significant decrease in V(max) values, but the effect was not as evident as at baseline. These findings could indicate that, in OCD patients, SRIs increase the rate of reuptake and decrease the inhibitory effect of PKC and that the two phenomena may be linked, the first perhaps depending upon the second.
Marazziti
Donatella
D
Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, University of Pisa, via Roma 67, 56100 Pisa, Italy. dmarazzi@psico.med.unipi.it
Dell'Osso
Liliana
L
Masala
Irene
I
Baroni
Stefano
S
Presta
Silvio
S
Giannaccini
Gino
G
Di Nasso
Elena
E
Mungai
Francesco
F
Lucacchini
Antonio
A
Cassano
Giovanni B
GB
D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000201:enzymology; D002648:Child; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D009771:Obsessive-Compulsive Disorder / Q000188:drug therapy* / Q000201:enzymology* / Q000523:psychology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000097:blood; D011569:Psychiatric Status Rating Scales; D012701:Serotonin / Q000097:blood; D017367:Selective Serotonin Reuptake Inhibitors / Q000627:therapeutic use*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004791:Enzyme Inhibitors; D011493:Protein Kinase C; D012701:Serotonin; D017367:Selective Serotonin Reuptake Inhibitors; D013755:Tetradecanoylphorbol Acetate