[Nano-ESI-MS/MS identification on apoptosis qssociated proteins induced by inhibiting ubiquitin-proteasome pathway].
CapLC-ESI-MS/MS and nano-ESI-MS/MS techniques were used to identify the apoptosis associated proteins induced by inhibiting the ubiquitin-proteasome pathway in Mo7e leukaemic cells. In 2-DE, spot H was found to initiate its overexpression at 2 h after the inhibition and reached its peak at 6 h. It was identified as Rho GDI beta protein after the tandem mass spectrum and after the sequence of its tryptic peptides were obtained by the ESI-MS/MS techniques. It was not revealed by peptide mass fingerprint using MALDI-TOF-MS. Other two spots induced by the inhibition appeared close to spot H were also revealed identical to Rho GDI brg;, possibly due to unknown modifications.
National Center of Biomedical Analysis, Academy of Military Medical Sciences, Beijing 100850, China. xmzhang@nic.bmi.ac.cn
Hong-Xia
HX
Wang
Bao-Feng
BF
Jin
Jie
J
Wang
Kun
K
He
Song-Cheng
SC
Yang
Bei-Feng
BF
Shen
Xue-Min
XM
Zhang
D017209:Apoptosis; D003546:Cysteine Endopeptidases; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000032:analysis*; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors*; D009363:Neoplasm Proteins / Q000032:analysis*; D046988:Proteasome Endopeptidase Complex; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014407:Tumor Cells, Cultured; D025801:Ubiquitin / Q000037:antagonists & inhibitors*; D020741:rho GTP-Binding Proteins / Q000032:analysis*
D020730:Guanine Nucleotide Dissociation Inhibitors; D009097:Multienzyme Complexes; D009363:Neoplasm Proteins; D025801:Ubiquitin; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D020741:rho GTP-Binding Proteins