Supercomplex formation between Mlh1-Mlh3 and Sgs1-Top3 heterocomplexes in meiotic yeast cells.
The genome of Saccharomyces cerevisia encodes four mismatch repair MutL proteins and these proteins form three heterocomplexes: Mlh1-Mlh2, Mlh1-Mlh3, and Mlh1-Pms1. Only, the Mlh1-Mlh3 heterocomplex has been implicated specifically in promotion of meiotic crossing-over. In this report, we show that yeast Mlh3 co-immunoprecipitates with Sgs1 helicase in sporulating cells at late stage of meiotic prophase I. Sgs1, a member of the RecQ DNA helicase family, appears to form a stable complex with topoisomerase III (Top3) during meiosis. We suggest that Mlh1-Mlh3 heterocomplex may act as a molecular matchmaker to coordinate Sgs1-Top3 complex in the resolution of meiotic recombination intermediates.
Wang
Ting-Fang
TF
Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan, ROC. tfwang@gate.sinica.edu.tw
Kung
Wen-Mei
WM
D048868:Adaptor Proteins, Signal Transducing; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D004265:DNA Helicases / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D008540:Meiosis; D000070957:MutL Protein Homolog 1; D000070956:MutL Proteins; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D011418:Prophase; D011485:Protein Binding; D053484:RecQ Helicases; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013997:Time Factors
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D005656:Fungal Proteins; C088859:MLH1 protein, S cerevisiae; C492408:MLH3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C090472:SGS1 protein, S cerevisiae; D000070957:MutL Protein Homolog 1; D000070956:MutL Proteins; D004265:DNA Helicases; D053484:RecQ Helicases; D004264:DNA Topoisomerases, Type I