Genomic DNA methylation-demethylation during aging and reinvigoration of Pinus radiata.
In animals, DNA methylation is related to gene silencing during ontogenic development. Little is known about DNA methylation in plants, although occasional changes in the DNA methylation state of specific gene promoters have been reported in angiosperms during some developmental processes. We found large differences in the extent of DNA methylation between meristematic areas of juvenile and mature Pinus radiata D. Don. trees, whereas differences in the extent of DNA methylation between differentiated tissues of juvenile and mature trees were small. In meristematic areas, there was a gradual decrease in extent of DNA methylation as the degree of reinvigoration increased. The observed changes in extent of DNA methylation during aging and reinvigoration indicate that reinvigoration could be a consequence of epigenetic modifications opposite in direction to those that occur during aging.
Lab. Fisiología Vegetal, Dpto. B.O.S., Facultad de Biología, Universidad de Oviedo, C/ Catedrático Rodrigo Uría s/n, E-33071 Oviedo, Spain.
Mario F
MF
Fraga
Roberto
R
Rodríguez
Maria Jesús
MJ
Cañal
D019175:DNA Methylation; D018744:DNA, Plant / Q000378:metabolism; D019075:Electrophoresis, Capillary; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D028223:Pinus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D014197:Trees / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
D018744:DNA, Plant