Unraveling lipid metabolism with microarrays: effects of arachidonate and docosahexaenoate acid on murine hepatic and hippocampal gene expression.
The functions, actions, and regulation of polyunsaturated fatty acids (PUFAs) are beginning to be unraveled. Mice were fed diets rich in either arachidonic acid, docosahexaenoic acid, or both. Liver and hippocampus tissue were then analyzed through a combined gene expression-, lipid-, and behavioral- profiling strategy. Novel hippocampal PUFA-molecular targets suggest that PUFA transcriptionally regulated genes with roles in appetite and learning.
Metabolic and Genomic Regulation, Nestle Research Center, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland.
Alvin
A
Berger
David M
DM
Mutch
J Bruce
JB
German
Matthew-Alan
MA
Roberts
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism / Q000494:pharmacology*; D001835:Body Weight / Q000235:genetics; D004032:Diet; D004041:Dietary Fats / Q000378:metabolism / Q000494:pharmacology; D004281:Docosahexaenoic Acids / Q000378:metabolism / Q000494:pharmacology*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000235:genetics*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
D004041:Dietary Fats; D008055:Lipids; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid