Kinetics and inhibition during the decolorization of reactive anthraquinone dyes under methanogenic conditions.
The objective of this study was to assess the biological decolorization of two reactive anthraquinone dyes (Reactive Blue 4, RB 4; Reactive Blue 19, RB 19) under methanogenic conditions. Using a mixed, methanogenic culture, batch assays were performed to evaluate both the rate and extent of color removal as well as any potential inhibition. The effect of initial dye, biomass, and organic feed concentration, as well as the effect of repetitive dye addition on color removal kinetics and culture inhibition were assessed. Overall, a lower rate and extent of color removal was observed in RB 4-amended cultures as opposed to the RB 19-amended cultures. For an incubation time of ca. 15 days and an initial dye concentration of 2000 mg/L, the extent of color removal was 50 and 95% for RB 4 and RB 19, respectively. Inhibition of acidogenesis and to a larger degree of methanogenesis, resulting in accumulation of volatile fatty acids, was observed in both RB 4- and RB 19-amended cultures. Although the degree of inhibition varied among the two dyes tested (RB 19 was more inhibitory than RB 4), an increase of inhibition was observed with increasing initial dye concentration. At an initial dye concentration of 500 mg/L or higher, methane production was lower than 6% of that of the control culture for both RB 4 and RB 19. However, color removal occurred despite culture inhibition.
Fontenot
E J
EJ
School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta 30332-0512, USA.
Beydilli
M I
MI
Lee
Y H
YH
Pavlostathis
S G
SG
D000880:Anthraquinones / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors; D004396:Coloring Agents / Q000378:metabolism*; D019605:Euryarchaeota / Q000502:physiology*; D007700:Kinetics; D009930:Organic Chemicals; D013783:Textile Industry; D014227:Triazines / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
D000880:Anthraquinones; D004396:Coloring Agents; D009930:Organic Chemicals; D014227:Triazines; C036598:procion blue MX-R; C024758:Remazol Brilliant Blue R