Fluorescein-based pI markers for capillary isoelectric focusing with laser-induced fluorescence detection.
We prepared a series of low-molecular-mass fluorescent ampholytes with narrow pI range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent pI markers, were examined. The pI values of new fluorescein-based pI markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass pI markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented.
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veverí 97, CZ-61142 Brno, Czech Republic. slais@iach.cz
Karel
K
Slais
Marie
M
Horká
Jana
J
Novácková
Zdenek
Z
Friedl
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods; D019793:Fluorescein; D016650:Fluorescein-5-isothiocyanate; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D007834:Lasers; D009994:Osmolar Concentration; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D012015:Reference Standards
D005456:Fluorescent Dyes; D011506:Proteins; D016650:Fluorescein-5-isothiocyanate; D019793:Fluorescein