Microwave assisted high yielding preparation of N-protected 2'-deoxyribonucleosides useful for oligonucleotide synthesis.
A rapid and high yielding method for the synthesis of precursors of synthons for DNA synthesis, N-protected 2'-deoxyribonucleosides is described, which occur under mild conditions using microwave irradiation. The desired material, N-protected nucleosides, was obtained in 93-96% yield in few minutes. The final products were then characterized by 1H-NMR and MALDI-TOF and compared with the standard samples. The method is amenable to small to moderate scale of synthesis.
Rao
N S
NS
Nucleic Acids Research Laboratory, Centre for Biochemical Technology, Delhi, India.
Kumar
P
P
Chauhan
V K
VK
Garg
B S
BS
Gupta
K C
KC
D003841:Deoxycytidine / Q000494:pharmacology; D003853:Deoxyribonucleosides / Q000138:chemical synthesis* / Q000494:pharmacology; D006868:Hydrolysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008872:Microwaves; D008956:Models, Chemical; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003853:Deoxyribonucleosides; D003841:Deoxycytidine