Bone induction in subcutaneous tissue in rats by a newly developed DNA-coated atelocollagen and bone morphogenetic protein.
A unique biomaterial, a mixture of DNA and collagen (DNA/collagen), was developed and its efficacy as a carrier matrix for bone morphogenetic protein (BMP) was evaluated histologically. The material was prepared as a composite of DNA from salmon milt and pepsin-digested type I collagen (atelocollagen) from bovine dermis. Phase-contrast and fluorescence microscopy showed atelocollagen fibres with DNA coating. The dose-response and time-course of bone induction by BMP in DNA/collagen (5 x 10 x 1 mm) in the subcutaneous tissue was investigated in 20 male Wistar rats. The BMP/DNA/collagen induced new bone in a dose-dependent manner (0, 25, 50 or 100 micrograms of BMP). Histological examination in the time-course study showed that the BMP (100 micrograms)/DNA/collagen induced bone formation, while the DNA/collagen alone resulted in the accumulation of fibroblasts. These results indicate that the DNA/collagen is effective as a carrier matrix for BMP. It provides a cell anchorage for differentiation of osteoblasts and is absorbed as bone matures.
Murata
M
M
Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Hokkaido, Japan. murata@hoku-iryo-u.ac.jp
Arisue
M
M
Sato
D
D
Sasaki
T
T
Shibata
T
T
Kuboki
Y
Y
D020341:Absorbable Implants; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage*; D002417:Cattle; D002454:Cell Differentiation; D020099:Coated Materials, Biocompatible; D003094:Collagen / Q000008:administration & dosage*; D004247:DNA / Q000737:chemistry*; D062109:Dermatologic Surgical Procedures; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D016503:Drug Delivery Systems; D046911:Macromolecular Substances; D008297:Male; D008567:Membranes, Artificial; D010006:Osteoblasts; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012474:Salmon; D013997:Time Factors
D019485:Bone Morphogenetic Proteins; D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D046911:Macromolecular Substances; D008567:Membranes, Artificial; C059821:atelocollagen; D003094:Collagen; D004247:DNA