N4 RNA polymerase II, a heterodimeric RNA polymerase with homology to the single-subunit family of RNA polymerases.
Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4 early promoter Pe3. These genes code for proteins of 269 and 404 amino acid residues with sequence similarity to the single-subunit, phage-like RNA polymerases. The genes encoding the N4 RNAPII subunits, as well as a synthetic construct encoding a fusion polypeptide, have been cloned and expressed. Both the individually expressed subunits and the fusion polypeptide reconstitute functional enzymes in vivo and in vitro.
Department of Molecular Genetics, The University of Chicago, Chicago, Illinois 60637, USA.
S H
SH
Willis
K M
KM
Kazmierczak
R H
RH
Carter
L B
LB
Rothman-Denes
D000595:Amino Acid Sequence; D025522:Bacteriophage N4 / Q000201:enzymology* / Q000502:physiology; D012321:DNA-Directed RNA Polymerases / Q000145:classification / Q000235:genetics; D019281:Dimerization; D004926:Escherichia coli / Q000254:growth & development / Q000821:virology*; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases