Oligoclonal Peyer's patch follicles in the terminal small intestine of cattle.
In small ruminants, the development of B cells differs from that in mice or in man. The anti-body repertoire is expanded in the Peyer's patches of the terminal ileum where each B-cell follicle is found by a few cells. To investigate the amount of founder clones in bovine ileal follicles, we have used sex mismatched cattle twins. These animals are chimeric due to placental anastomoses. Y-chromosome targeted in situ hybridization was used to trace donor-derived cells of the male genotype in a female recipient (called a freemartin). A strong clustering of lymphoid cells originating from either twin was seen in the ileal Peyer's patches (IPPs). Furthermore, the follicles displayed a low amount of immunoglobulin heavy chain gene configurations in comparison with the splenic or jejunal follicles. These findings strongly suggest that as in sheep, the B-cell follicles in cattle IPPs develop oligoclonally.
Division of Anatomy, Department of Basic Veterinary Sciences, University of Helsinki, FIN-00014 Helsinki, Finland.
Mikael
M
Niku
Tiina
T
Pessa-Morikawa
Leif C
LC
Andersson
Antti
A
Iivanainen
D000818:Animals; D000833:Animals, Suckling; D001402:B-Lymphocytes / Q000276:immunology*; D002417:Cattle; D002465:Cell Movement; D002678:Chimera; D005260:Female; D005611:Freemartinism / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D017403:In Situ Hybridization; D007421:Intestine, Small / Q000254:growth & development / Q000276:immunology*; D010581:Peyer's Patches / Q000276:immunology*; D014427:Twins; D014998:Y Chromosome
D007143:Immunoglobulin Heavy Chains