Human serum albumin adsorption onto a-SiC:H and a-C:H thin films deposited by plasma enhanced chemical vapor deposition.
In the present paper, we report the study of the adsorption behavior of a model protein such as human serum albumin (HSA) onto surfaces of a-SiC:H and a-C:H thin films deposited by using the plasma-enhanced chemical vapor deposition (PECVD) technique. The surface composition and surface energy of the various substrates as well as the evaluation of the adsorbed amount of protein has been carried out by means of X-ray photoelectron spectroscopy (XPS) and contact angle measurements. It has been found that HSA tends to preferentially adsorb on Si-rich surfaces, as far as the relative amount of adsorbed HSA decreases with increasing S-C concentration. Preliminary elements of mechanistic models are proposed for the correlation between chemical factors and the observed protein adsorption behavior.
Department of Chemistry, University of Catania, v. A.Doria, 6-95125 Catania, Italy.
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Auditore
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Satriano
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Coscia
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Ambrosone
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Parisi
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Marletta
D000327:Adsorption; D002244:Carbon; D017554:Carbon Compounds, Inorganic; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D005898:Glass; D006801:Humans; D006859:Hydrogen; D008422:Materials Testing / Q000379:methods*; D012709:Serum Albumin / Q000737:chemistry*; D012825:Silicon; D017655:Silicon Compounds; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D017554:Carbon Compounds, Inorganic; D020099:Coated Materials, Biocompatible; D012709:Serum Albumin; D017655:Silicon Compounds; D002244:Carbon; D006859:Hydrogen; C022088:silicon carbide; D012825:Silicon