D-RNAi (messenger RNA-antisense DNA interference) as a novel defense system against cancer and viral infections.
D-RNAi (Messenger RNA-antisense DNA interference), a novel posttranscriptional phenomenon of silencing gene expression by transfection of mRNA-aDNA hybrids, was originally observed in the effects of bcl-2 on phorbol ester-induced apoptosis in human prostate cancer LNCaP cells. This phenomenon was also demonstrated in chicken embryos and a human CD4(+) T cell line, H9. The in vivo transduction of beta-catenin D-RNAi was shown to knock out more than 99% endogenous beta-catenin gene expression, while the in cell transfection of HIV-1 D-RNAi homolog rejected viral gene replication completely. D-RNAi was found to have long-term gene knockout effects resulting from a posttranscriptional gene silencing mechanism that may involve the homologous recombination between intracellular mRNA and the mRNA components of a D-RNAi construct. These findings provide a potential intracellular defense system against cancer and viral infections.
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Lin
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Sukasweang
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Chuong
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Rasheed
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Ying
D016373:DNA, Antisense / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism*; D020868:Gene Silencing; D006801:Humans; D009369:Neoplasms / Q000235:genetics* / Q000628:therapy; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
D016373:DNA, Antisense; D018076:DNA, Complementary; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D022661:RNA, Untranslated