[RAPD markers linked to the stripe rust resistance gene Yr5 in the wheat variety Triticum spelta album].
A total of 520 10-mer random primers were used to identify the RAPD markers linked to the Yr5 gene between the near-isogenic line Yr5/6 x Avocet S and recurrent parent Avocet S. Three polymorphic DNA fragments, S1496(761), S1453(880) and S1418(1950), were found linked to the Yr5 gene. In which the genetic distance between S1496(761) and Yr5 gene was 2.7 cM. The fragment S1496(761) was recovered from the gel and cloned and sequenced. A pair of specific PCR primers was designed based on the sequence. The specific primers amplified the same fragment about 761bp as the random primer S1496 did. Because the primers could amplify another non-specific fragment, the PCR products must be analyzed by electrophoresis on polyacrylamide gels.
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China.
Ming
M
Zhong
Yong-Chun
YC
Niu
Shi-Chang
SC
Xu
Li-Ren
LR
Wu
D001487:Basidiomycota / Q000254:growth & development; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics*
D018744:DNA, Plant; D005819:Genetic Markers