Specific 14-3-3 isoform detection and immunolocalization in prion diseases.
14-3-3 proteins are involved in signalling processes in neuronal cells. Using isoform-specific antibodies we have examined the variation in 14-3-3 isoform neurolocation in normal and scrapie-infected murine brain and show that in defined areas of the brain there are significant changes associated with the pathology of the disease process. The appearance of 14-3-3 proteins in the cerebrospinal fluid (CSF) is a consequence of neuronal disease and the detection of specific isoforms of the 14-3-3 proteins in the CSF is characteristic of some neurodegenerative diseases. In this study, monitoring specifically for the gamma 14-3-3 isoform in the CSF by both Western-blot analysis and ELISA we can show a level of correlation between the assays.
Department of Biomedical Sciences, University of Edinburgh, George Square, Edinburgh EH8 9XD, UK. hbaxter@ed.ac.uk
H C
HC
Baxter
J R
JR
Fraser
W-G
WG
Liu
J L
JL
Forster
S
S
Clokie
P
P
Steinacker
M
M
Otto
E
E
Bahn
J
J
Wiltfang
A
A
Aitken
D048948:14-3-3 Proteins; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D019636:Neurodegenerative Diseases / Q000134:cerebrospinal fluid / Q000175:diagnosis; D009474:Neurons / Q000201:enzymology; D017096:Prion Diseases / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D015398:Signal Transduction; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis* / Q000134:cerebrospinal fluid
D048948:14-3-3 Proteins; D014446:Tyrosine 3-Monooxygenase