Size and sequence variability of the Arabis mosaic virus protein 2A.
The RNA 2 of the nepovirus Arabis mosaic virus (ArMV) encodes a polyprotein from which protein 2A is released by proteolytic cleavage at the N-terminus. The 2A gene of 19 ArMV isolates from different geographical origin and 9 distinct natural hosts was amplified by RT/PCR and subsequently cloned and sequenced. These 19 isolates and those from databanks were classified into four groups based on the size of the protein 2A which ranged from 233 to 280 amino acids, and sequence identities. Sequence variability was mainly located in the N-terminus of the proteins, whereas the core region and the C-terminus were conserved.
Wetzel
Thierry
T
Centrum Gruene Gentechnik, Staatliche Lehr- und Forschungsanstalt, Breitenweg 71, D-67435 Neustadt an der Weinstrasse, Germany. twetzel.slfa-nw@agrarinfo.rlp.de
Fuchs
M
M
Bobko
M
M
Krczal
G
G
D000595:Amino Acid Sequence; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry*
D014764:Viral Proteins; C400561:protein 2A, nepovirus