Novel approach for the effective determination of DNA scission site using the Sanger method.
This paper describes the effective determination of DNA scission site using a novel approach that is based on the Sanger method of nucleotide sequencing. The DNA scission site is determined by contrast with the nucleotide sequence of the DNA. Here, instead of the traditional Maxam-Gilbert method for the determination of the DNA sequence, we utilized the Sanger method and studied its effectiveness in the determination of DNA scission sites. Using this method, the determination of DNA scission site becomes more facile and exact. And the total time for the determination is reduced nearly by half in comparison to the Maxam-Gilbert method. Further advantages of this novel approach include the reduced risks of radiation exposure for researchers and contamination of the apparatus.
Graduate School of Science and Engineering, Ibaraki University, Mito, Ibaraki 310-8512, Japan.
Fuminori
F
Sakamoto
Eiji
E
Suzuki
Yuki
Y
Fujii
D001483:Base Sequence; D003514:Cyclohexylamines / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005821:Genetic Techniques; D006801:Humans; D008969:Molecular Sequence Data; D016273:Occupational Exposure / Q000517:prevention & control; D010957:Plasmids / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000379:methods*
C421444:1,3,5-triaminocyclohexane; D003514:Cyclohexylamines; D004247:DNA