[Abnormal expression of the FHIT gene in human primary gastrointestinal cancers].
OBJECTIVE
To evaluate the relationship between FHIT gene and gastrointestinal cancers.


METHODS
Matched normal and cancerous tissues from 26 patients with primary gastric carcinoma and 30 patients with colorectal carcinoma were analysed by nested polymerase chain reaction(RT-PCR) and direct sequencing of the products.


RESULTS
Seven of 26(26.9%) gastric cancer tissues and eight of 30(26.7%) colorectal cancer tissues expressed aberrant FHIT transcripts, but no aberrant FHIT transcript was observed in all the matched normal tissues. Sequence analysis of the aberrant transcripts of 2 gastric cancer tissues(G8T, G25T) and 2 colorectal cancer tissues(C5T, C26T) confirmed the absence of 1 to 3 exons of FHIT gene.


CONCLUSIONS
The expression of aberrant FHIT transcripts is common and tumor-specific in gastrointestinal cancers and abnormalities of FHIT gene may be associated with gastrointestinal tumorigenesis.
Xiangya Hospital, Hunan Medical University, Changsha 410008.
X M
XM
Duan
G Y
GY
Zhang
E S
ES
Wu
D017766:Acid Anhydride Hydrolases; D003110:Colonic Neoplasms / Q000235:genetics*; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000235:genetics*; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase