Alkane-1,2-diol-based glycosides and fatty glycosides and wax esters in Roseiflexus castenholzii and hot spring microbial mats.
The lipid composition of Roseiflexus castenholzii, a thermophilic filamentous phototrophic bacterium related to uncultivated filamentous phototrophic bacteria that predominate in hot spring microbial mats, is reported. R. castenholzii lipid extracts were dominated by components characterized by alkane-1-ol-2-alkanoate moieties glycosidically bonded to a C(6) sugar. Similar fatty glycosides, with an additional fatty acid esterified, were detected by HPLC-MS. R. castenholzii also produces a suite of wax esters ranging from 37 to 40 carbon atoms in length. In lipid extracts from two nonsulfidic hot spring microbial mats, similar alkane-1,2-diol-based lipids were detected in minor amounts. R. castenholzii lipids are compared to lipids of mats and other thermophilic mat isolates.
van ver Meer
Marcel T J
MT
Netherlands Institute for Sea Research (NIOZ), Department of Marine Biogeochemistry and Toxicology, P.O. Box 59, 1790 AB Den Burg, Texel, The Netherlands. mmeer@nioz.nl
Schouten
Stefan
S
Hanada
Satoshi
S
Hopmans
Ellen C
EC
Damsté
Jaap S Sinninghe
JS
Ward
David M
DM
D000473:Alkanes / Q000032:analysis; D019414:Chlorobi / Q000737:chemistry*; D002849:Chromatography, Gas; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D008401:Gas Chromatography-Mass Spectrometry; D006027:Glycosides / Q000032:analysis*; D006358:Hot Temperature; D008055:Lipids / Q000032:analysis*; D014885:Waxes / Q000032:analysis
D000473:Alkanes; D004952:Esters; D005227:Fatty Acids; D006027:Glycosides; D008055:Lipids; D014885:Waxes