Histamine is involved in gastric vasodilation during acid back diffusion via activation of sensory neurons.
Protective vasodilation during acid back diffusion into the rat gastric mucosa depends on activation of sensory neurons and mast cell degranulation with histamine release. We hypothesized that these two mediator systems interact and that histamine partly exerts its effect via sensory nerves. Gastric blood flow (GBF) and luminal histamine were measured in chambered stomachs, and mast cell numbers were assessed by morphometry. Ablation of sensory neurons and depletion of mast cells were produced by pretreatment with capsaicin or dexamethasone, respectively. Mucosal exposure to 1.5 M NaCl and then to pH 1.0 saline in ablated and control rats caused increased luminal histamine and reduced numbers of mast cells. Enterochromaffin-like cell marker pancreastatin remained unchanged. Only control rats responded with an increase in GBF. Capsaicin stimulation (640 microM) of the undamaged mucosa induced identical increase in GBF and unchanged mast cell mass in normal and dexamethasone-treated rats. Increase in GBF after topical exposure to histamine (30 mM) in rats pretreated with capsaicin or a calcitonin gene-related peptide (CGRP)(1) antagonist human CGRP(8-37) or exposed to the calcium pore blocker ruthenium red was less than one-half of that in control rats. These data suggest that mast cell-derived histamine is involved in gastric vasodilatation during acid back diffusion partly via sensory neurons.
Departments of Surgery, Norwegian University of Science and Technology, N-7006 Trondheim, Norway. Astrid.Rydning@rit.no
Astrid
A
Rydning
Oddveig
O
Lyng
Sture
S
Falkmer
Jon Erik
JE
Grønbech
D000818:Animals; D002211:Capsaicin / Q000494:pharmacology; D015550:Cell Degranulation / Q000502:physiology; D004058:Diffusion; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D008407:Mast Cells / Q000502:physiology; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013270:Stomach / Q000098:blood supply* / Q000294:innervation; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*
D006632:Histamine; D002211:Capsaicin