Poliovirus transcytosis through M-like cells.
During the digestive-tract phase of infection, poliovirus (PV) is found in the oropharynx and the intestine. It has been proposed that PV enters the organism by crossing M cells, which are scattered in the epithelial sheet covering lymphoid follicles of Peyer's patches. However, PV translocation through M cells has never been demonstrated. A model of M-like cells has been previously developed using monolayers of polarized Caco-2 enterocytes cocultured with lymphocytes isolated from Peyer's patches. In this model, lymphoepithelial interactions trigger the appearance of epithelial cells having morphological and functional characteristics of M cells. We have demonstrated efficient, temperature-dependent PV transcytosis in Caco-2 cell monolayers containing M-like cells. This experimental evidence is consistent with M cells serving as gateways allowing PV access to the basal face of enterocytes, the underlying immune follicle cells, and PV transport toward mesenteric lymph nodes.
Ouzilou
Laurent
L
Génétique Virale-NRSN, Département Neuroscience1, Interactions Lymphoépithéliales2 and Plate-forme de microscopie électronique3, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris cedex 15, France.
Caliot
Elise
E
Génétique Virale-NRSN, Département Neuroscience1, Interactions Lymphoépithéliales2 and Plate-forme de microscopie électronique3, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris cedex 15, France.
Pelletier
Isabelle
I
Génétique Virale-NRSN, Département Neuroscience1, Interactions Lymphoépithéliales2 and Plate-forme de microscopie électronique3, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris cedex 15, France.
Prévost
Marie-Christine
MC
Génétique Virale-NRSN, Département Neuroscience1, Interactions Lymphoépithéliales2 and Plate-forme de microscopie électronique3, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris cedex 15, France.
Pringault
Eric
E
Génétique Virale-NRSN, Département Neuroscience1, Interactions Lymphoépithéliales2 and Plate-forme de microscopie électronique3, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris cedex 15, France.
Colbère-Garapin
Florence
F
Génétique Virale-NRSN, Département Neuroscience1, Interactions Lymphoépithéliales2 and Plate-forme de microscopie électronique3, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris cedex 15, France.
D000818:Animals; D018938:Caco-2 Cells; D006801:Humans; D008214:Lymphocytes / Q000821:virology*; D051379:Mice; D009068:Movement; D010581:Peyer's Patches / Q000276:immunology; D011051:Poliomyelitis / Q000821:virology*; D017955:Poliovirus / Q000254:growth & development / Q000378:metabolism*; D014779:Virus Replication