Physiological responses to copper in giant clams: a comparison of two methods in revealing effects on photosynthesis in zooxanthellae.
In the present work physiological responses to a short term, sub-lethal exposure to copper (5 microg Cu2 l(-1) were studied on two species of giant clams, Tridacna gigas and Hippopus hippopus. All giant clams (fam. Tridacnidae) maintain a symbiotic relationship with photo-synthesising microalgae (zooxanthellae) and the phytotoxic effects of this copper concentration were approached with two different end points: changes in oxygen production and maximal quantum yield (Fv/Fm). Additionally we measured respiration, whereby Pg:R ratios could be calculated for the system (algae and clam) and used as an indicator of stress. Cu accumulation was only determined for T. gigas and showed significantly higher tissue concentrations in exposed specimens (11.3 microg Cu g(-1) dry wt) compared to the controls (4.2 microg Cu g(-1)). Both species displayed significantly reduced Pg:R ratios due to exposure, mainly explained by lowered gross production. No indication of stress was shown in the fluorescence measurements and possible explanations are discussed in the paper. We propose that inhibitions in the slow kinetics of photosynthesis were not captured by this fluorescence parameter. Accordingly we suggest that measurements of maximum fluorescence yield should be accompanied by complementary parameters when studying pollutants with unclear phytotoxic mechanisms.
Department of Systems Ecology, Stockholm University, Sweden. tina@system.ecology.su.se
Tina
T
Elfwing
Eva
E
Blidberg
Michael
M
Tedengren
D000818:Animals; D049872:Bivalvia / Q000502:physiology*; D003300:Copper / Q000633:toxicity*; D004781:Environmental Exposure / Q000009:adverse effects*; D004784:Environmental Monitoring / Q000379:methods; D056890:Eukaryota / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D010679:Philippines; D010788:Photosynthesis / Q000502:physiology*; D012119:Respiration; D012623:Seawater; D013559:Symbiosis / Q000502:physiology; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D003300:Copper; D010100:Oxygen