Effect of cryoglobulin and crystalcryoglobulin on TNF-alpha production by normal human monocytes.
OBJECTIVE
We recovered an IgG1-kappa cryocrystalglobulin in synovial fluid and membrane specimens from a patient with destructive arthropathy. In the present study, we investigated its proinflammatory properties by measuring its effects on TNF-alpha production by normal human monocytes.


METHODS
Normal human monocytes isolated by plastic adhesion were cultured in microtiter plates. Adherent monocytes were cultured for 6, 8, and 24 hours with sterile cryocrystalglobulin (150 microg/mL and 2 mg/mL), type I noncrystallised cryoglobulin (same concentrations), monosodium urate (MSU) crystals (2 mg/mL), LPS (10 microg/mL), or medium alone. Supernatant TNF-a concentrations were assayed using an ELISA.


RESULTS
Cryocrystalglobulin had no effect on TNF-alpha production by normal human monocytes. Noncrystallised cryoglobulin increased TNF-alpha levels in supernatants in a time-dependent and concentration-dependent fashion. This increase was significantly less marked than the increases achieved with MSU crystals or LPS.


CONCLUSIONS
IgG1kappa cryocrystalglobulin has no effect on TNF-alpha production by normal human monocytes. Fc region changes within the cryocrystalglobulin molecule may explain this finding.
Laboratoire de radiologie expérimentale et de physiopathologie articulaire, hĵpital Lariboisière, Assistance publique-hĵpitaux de Paris, AP-HP, France.
Barbara
B
Bucki
Romuald
R
Champy
Lucille
L
Musset
Thomas
T
Bardin
Frédéric
F
Lioté
D002478:Cells, Cultured; D003450:Cryoglobulins / Q000737:chemistry / Q000494:pharmacology*; D003460:Crystallization; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism*; D012016:Reference Values; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D003450:Cryoglobulins; D007074:Immunoglobulin G; D014409:Tumor Necrosis Factor-alpha