The Haemophilus influenzae Hap autotransporter binds to fibronectin, laminin, and collagen IV.
Nontypeable Haemophilus influenzae (NTHI) initiates infection by colonizing the upper respiratory tract mucosa. NTHI disease frequently occurs in the context of respiratory tract inflammation, where organisms encounter damaged epithelium and exposed basement membrane. In this study, we examined interactions between the H. influenzae Hap adhesin and selected extracellular matrix proteins. Hap is an autotransporter protein that undergoes autoproteolytic cleavage, with release of the adhesive passenger domain, Hap(s), from the bacterial cell surface. We found that Hap promotes bacterial adherence to purified fibronectin, laminin, and collagen IV and that Hap-mediated adherence is enhanced by inhibition of autoproteolysis. Adherence is inhibited by pretreatment of bacteria with a polyclonal antiserum recognizing Hap(s). Purified Hap(s) binds with high affinity to fibronectin, laminin, and collagen IV but not to collagen II. Binding of Hap(s) to fibronectin involves interaction with the 45-kDa gelatin-binding domain but not the 30-kDa heparin-binding domain of fibronectin. Taken together, these observations suggest that interactions between Hap and extracellular matrix proteins may play an important role in NTHI colonization of the respiratory tract.
Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Doran L
DL
Fink
Bruce A
BA
Green
Joseph W
JW
St  Geme
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D024141:Collagen Type IV / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism; D006192:Haemophilus Infections / Q000209:etiology; D006193:Haemophilus influenzae / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007797:Laminin / Q000378:metabolism; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012697:Serine Endopeptidases
D001425:Bacterial Outer Membrane Proteins; D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; C091552:Hap protein, Hemophilus influenzae; D007797:Laminin; D012697:Serine Endopeptidases