Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting.
The sorting of transmembrane proteins (e.g., cell surface receptors) into the multivesicular body (MVB) pathway to the lysosomal/vacuolar lumen requires the function of the ESCRT protein complexes. The soluble coiled-coil-containing proteins Vps2, Vps20, Vps24, and Snf7 are recruited from the cytoplasm to endosomal membranes where they oligomerize into a protein complex, ESCRT-III. ESCRT-III contains two functionally distinct subcomplexes. The Vps20-Snf7 subcomplex binds to the endosomal membrane, in part via the myristoyl group of Vps20. The Vps2-Vps24 subcomplex binds to the Vps20-Snf7 complex and thereby serves to recruit additional cofactors to this site of protein sorting. We provide evidence for a role for ESCRT-III in sorting and/or concentration of MVB cargoes.
Babst
Markus
M
Department of Cellular and Molecular Medicine and Howard Hughes Medical Institute, School of Medicine, University of California, San Diego, La Jolla 92093, USA.
Katzmann
David J
DJ
Estepa-Sabal
Eden J
EJ
Meerloo
Timo
T
Emr
Scott D
SD
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism* / Q000648:ultrastructure; D002451:Cell Compartmentation / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D056827:Endosomal Sorting Complexes Required for Transport; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure; D004926:Escherichia coli; D005057:Eukaryotic Cells / Q000378:metabolism* / Q000648:ultrastructure; D005656:Fungal Proteins / Q000378:metabolism* / Q000648:ultrastructure; D008247:Lysosomes / Q000378:metabolism / Q000648:ultrastructure; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008854:Microscopy, Electron; D009154:Mutation / Q000502:physiology; D009687:Nuclear Proteins; D010447:Peptide Hydrolases / Q000378:metabolism; D021381:Protein Transport / Q000502:physiology*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D022161:Transport Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D021601:trans-Golgi Network / Q000378:metabolism / Q000648:ultrastructure
D002352:Carrier Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D005656:Fungal Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D009687:Nuclear Proteins; C083890:SNF7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D010447:Peptide Hydrolases