LC-MS determination of urinary toluenediamine in workers exposed to toluenediisocyanate.
To improve the biological monitoring method for 2,6- and 2,4-toluenediisocyanate (TDI) exposure, we developed a simple and rapid method for analysis of the corresponding urinary metabolites, 2,6- and 2,4-toluenediamine (TDA) using liquid chromatograph-mass spectrometry (LC-MS). One ml of urine was hydrolyzed at 100 degrees C for 1.5 h with H(2)SO(4). Alkalinized hydrolysate was extracted with dichloromethane (DCM) and analyzed by atmospheric pressure chemical ionization (APCI) LC-MS, in positive-ion mode. The mass spectra of TDA isomers showed the protonated molecule [M+H](+), at m/z 123 as the base peak. Calibration curves of 2,6-TDA were linear up to 400 microg/l. TDA isomers in urine of exposed workers as determined by LC-MS correlated well with those obtained by gas chromatography-mass spectrometry. 2,6- and 2,4-TDA were not detected in non-exposed subjects, whereas exposed workers showed urinary levels up to 250 and 63 microg/l, respectively.
Tokyo Rosai Hospital, Occupational Poisoning Center, 13-21, Omoriminami-4, Ota-Ku, 143-0013 Tokyo, Japan. opc@msa.biglobe.ne.jp
Tadashi
T
Sakai
Y
Y
Morita
Y
Y
Kim
Y X
YX
Tao
D002853:Chromatography, Liquid / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D019570:Inhalation Exposure; D013058:Mass Spectrometry / Q000379:methods; D016273:Occupational Exposure; D010655:Phenylenediamines / Q000652:urine*; D014051:Toluene 2,4-Diisocyanate / Q000493:pharmacokinetics*
D010655:Phenylenediamines; D014051:Toluene 2,4-Diisocyanate; C026942:2,6-diisocyanatotoluene; C025235:2,6-diaminotoluene; C010914:2,4-diaminotoluene