Susceptibility of nontypeable Haemophilus influenzae to human beta-defensins is influenced by lipooligosaccharide acylation.
Nontypeable Haemophilus influenzae (NTHI) lipooligosaccharide htrB mutants exhibited greater than 45-fold-increased sensitivity to human beta-defensin 2 (HBD-2) compared to the wild type. Complementation by htrB in trans to acylation competence reversed this increased sensitivity. In contrast, NTHI was more susceptible to HBD-3 and showed no changes in sensitivity as a result of lipooligosaccharide mutations in oligosaccharide and lipid A biosynthesis genes.
Department of Pediatrics, The University of Iowa, Iowa City, Iowa 52242, USA.
Timothy D
TD
Starner
W Edward
WE
Swords
Michael A
MA
Apicella
Paul B
PB
McCray
D000215:Acylation; D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006193:Haemophilus influenzae / Q000187:drug effects* / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008050:Lipid A / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism*; D009154:Mutation; D023083:beta-Defensins / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C422950:DEFB103A protein, human; C106641:DEFB4A protein, human; D008050:Lipid A; D008070:Lipopolysaccharides; D023083:beta-Defensins; C023023:lipid-linked oligosaccharides; D000217:Acyltransferases; C464646:HtrB protein, Haemophilus influenzae