Matrilin-2 interacts with itself and with other extracellular matrix proteins.
Matrilin-2 is a component of extracellular filamentous networks. To study the interactions by which it can be integrated into such assemblies, full-length and truncated forms of matrilin-2 were recombinantly expressed in HEK-293 cells and purified from conditioned medium. The recombinant proteins, when used in interaction assays, showed affinity to matrilin-2 itself, but also to other collagenous and non-collagenous extracellular matrix proteins. The interaction between matrilin-2 and collagen I was studied in greater detail and could be shown to occur at distinct sites on the collagen I molecule and to have a K (D) of about 3 x 10(-8) M. Interactions with some non-collagenous protein ligands were even stronger, with matrilin-2 binding to fibrillin-2, fibronectin and laminin-1-nidogen-1 complexes, with K (D) values in the range of 10(-8)-10(-11) M. Co-localization of matrilin-2 with these ligands in the dermal-epidermal basement membrane, in the microfibrils extending from the basement membrane into the dermis, and in the dermal extracellular matrix, indicates a physiological relevance of the interactions in the assembly of supramolecular extracellular matrix structures.
Institute for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, D-50931 Cologne, Germany.
Dorothea
D
Piecha
Charlotte
C
Wiberg
Matthias
M
Mörgelin
Dieter P
DP
Reinhardt
Ferenc
F
Deák
Patrik
P
Maurer
Mats
M
Paulsson
D001483:Base Sequence; D002460:Cell Line; D024042:Collagen Type I / Q000378:metabolism; D017931:DNA Primers; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D064235:Matrilin Proteins; D008854:Microscopy, Electron; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D020349:Surface Plasmon Resonance
D024042:Collagen Type I; D017931:DNA Primers; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D008024:Ligands; C577541:MATN2 protein, human; D064235:Matrilin Proteins; D011994:Recombinant Proteins