[Characteristics of defective strains of methicillin resistance Staphylococcus aureus that do not produce coagulase or CF].
The aim of the study was to estimate frequency of coagulase-negative and CF-negative strains among methicillin-resistant Staphylococcus aureus (MRSA) and to assess their homogeneicity in respect of genotype, phagotype and drug resistance pattern. A total of 186 MRSA strains collected from different hospitals in Gdańsk region were studied. Gens: nuc, mecA, and coa were identified by PCR method. The coagulase tube test for staphylocoagulase and the slide test for clumping factor were used. Coagulase-negative and CF-negative MRSA strains were confirmed by PCR-RFLP method of coa gene; phage typing and drug resistance pattern were evaluated by disc diffusion test. The results of the study showed low frequency of both coagulase-negative and CF-negative MRSA strains (7.25% and 3.76% respectively). Among MRSA population tested the simultaneous occurrence of the strains lacking coagulase and clumping factor was not observed. All coagulase negative MRSA had coagulase gene (coa) and differed from CF-negative strains in respect of coa gene.
Katedra i Zakład Mikrobiologii Lekarskiej Akademii Medycznej w Gdańsku.
Katarzyna
K
Garbacz
Lidia
L
Piechowicz
Katarzyna
K
Wiśniewska
Janusz
J
Galiński
D003030:Coagulase / Q000235:genetics / Q000378:metabolism*; D003428:Cross Infection / Q000382:microbiology; D005838:Genotype; D006801:Humans; D016106:Methicillin Resistance / Q000502:physiology*; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000201:enzymology* / Q000235:genetics
D003030:Coagulase