Structural analysis of the lipopolysaccharide from Neisseria meningitidis strain BZ157 galE: localisation of two phosphoethanolamine residues in the inner core oligosaccharide.
The structure of the phase-variable lipopolysaccharide (LPS) from the group B Neisseria meningitidis strain BZ157 galE was elucidated. The structural basis for the LPS's variation in reactivity with a monoclonal antibody (MAb) B5 that has specificity for the presence of phosphoethanolamine (PEtn) at the 3-position of the distal heptose residue (HepII) was established. The structure of the O-deacylated LPS was deduced by a combination of monosaccharide analyses, nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. These analyses revealed the presence of a novel inner core oligosaccharide (OS) structure in the MAb B5 reactive (B5+) LPS that contained two PEtn residues simultaneously substituting the 3- and 6-positions of the HepII residue. The determination of this structure has identified a further degree of variability within the inner core OS of meningococcal LPS that could contribute to the interaction of meningococcal strains with their host.
Cox
Andrew D
AD
Institute for Biological Sciences, National Research Council, 100 Sussex Drive, Rm 3089, ON, K1A 0R6, Ottawa, Canada. andrew.cox@nrc.ca
Li
Jianjun
J
Brisson
Jean-Robert
JR
Moxon
E Richard
ER
Richards
James C
JC
D000215:Acylation; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004983:Ethanolamines / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D014644:Genetic Variation; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry / Q000276:immunology; D010446:Peptide Fragments / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014534:UDPglucose 4-Epimerase / Q000235:genetics
D000911:Antibodies, Monoclonal; D004983:Ethanolamines; D008070:Lipopolysaccharides; D019081:O Antigens; D010446:Peptide Fragments; C005448:phosphorylethanolamine; D014534:UDPglucose 4-Epimerase; C020551:galactose epimerase