Detection of human papillomavirus DNA in urine specimens from human immunodeficiency virus-positive women.
Human immunodeficiency virus (HIV)-positive women may represent one of the fastest-growing populations at risk for acquiring cervical cancer and thus require frequent screening. The purpose of the present studies was to validate a PCR-based urine assay by comparing detection and genotyping of human papillomavirus (HPV) DNA in urine samples and matching cervical swab specimens of HIV-positive women. Despite a difference in amplifiability, the prevalence of any HPV genotype (58% for the cervical swab specimens and 48% for the urine specimens) was not significantly different in this population. The levels of concordance were 70, 71, and 78% for detection of any HPV type, any high-risk HPV type, or any low-risk HPV type in the two specimen types, respectively. While instances of discordant detection were greater for the cervical swab specimens than for the urine specimens, this was not statistically significant. The distributions of HPV genotypes were similar in the cervix and the urine for the majority of types examined. Importantly, detection of HPV DNA in urine was associated with an abnormal Papanicolaou smear to the same extent that detection of HPV DNA in a cervical swab specimen was. These data provide preliminary support for the proposal to use urine testing as a primary or secondary screening tool for cervical cancer in HIV-positive women or as an epidemiological tool. Additional studies with larger sample sizes must be conducted in order to further verify these findings.
Brinkman
Joeli A
JA
Department of Microbiology. Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112-2822, USA. jbrink@lsuhsc.edu
Jones
W Elizabeth
WE
Gaffga
Ann M
AM
Sanders
Jonathan A
JA
Chaturvedi
Anil K
AK
Slavinsky III
Joseph
J
Clayton
John L
JL
Dumestre
Jeanne
J
Hagensee
Michael E
ME
D000328:Adult; D002584:Cervix Uteri / Q000821:virology; D004279:DNA, Viral / Q000652:urine*; D005260:Female; D006679:HIV Seropositivity; D006801:Humans; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D013048:Specimen Handling; D014412:Tumor Virus Infections / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000821:virology; D002578:Uterine Cervical Dysplasia
D004279:DNA, Viral