Evaluation of restriction fragment length polymorphism analysis of 16S rDNA as a tool for genomovar characterisation within the Burkholderia cepacia complex.
A total of 154 Burkholderia cepacia complex strains, isolated from cystic fibrosis and non-cystic fibrosis patients and the environment, representing all nine genomovars and a putative tenth, were analysed by 16S rDNA-restriction fragment length polymorphism using the restriction enzymes AluI, CfoI and DdeI. Examining this diverse strain collection resulted in very diverse restriction patterns. Only B. cepacia genomovar VI could be identified unambiguously. The same restriction patterns were observed for B. cepacia genomovars I and III and approximately half of the Burkholderia ambifaria, B. anthina and B. pyrrocinia strains. Burkholderia vietnamiensis and B. ubonensis, a putative tenth B. cepacia complex genomovar, shared identical restriction profiles. The majority of Burkholderia multivorans and B. stabilis isolates generated a unique restriction pattern, but two strains of each showed divergent restriction profiles which were also observed in other genomovars.
Laboratory for Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ghent University, Belgium.
Karen
K
Vermis
Christoph
C
Vandekerckhove
Hans J
HJ
Nelis
Peter A R
PA
Vandamme
D000818:Animals; D015373:Bacterial Typing Techniques; D019121:Burkholderia Infections / Q000382:microbiology; D016956:Burkholderia cepacia / Q000145:classification* / Q000235:genetics*; D003550:Cystic Fibrosis / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D004783:Environmental Microbiology; D005838:Genotype; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D015252:Deoxyribonucleases, Type II Site-Specific