Microarray analysis of VEGF-responsive genes in myometrial endothelial cells.
There is evidence that the vasculature of different organs display different functional characteristics in response to cytokines and growth factors. The aim of this study was to use cDNA gene expression microarray to analyse changes in gene expression following stimulation of myometrial microvascular endothelial cells (MMECs) with vascular endothelial growth factor (VEGF). Primary isolates of MMECs were obtained from fresh hysterectomy specimens and purified with magnetic beads. Cells were stimulated with 15 ng/ml VEGF for 3, 6 and 12 h, and two unstimulated experiments served as controls. A total of six arrays was performed over these time-points. A total of 110 genes were identified as up-regulated by VEGF, 19% of which (21 genes) have previously been reported as up-regulated by VEGF or by angiogenesis. Among the novel genes to be up-regulated by VEGF were brain-derived growth factor, oxytocin receptor and estrogen sulphotransferase. The significance of the genes identified in the physiological and pathological functioning of the myometrial vasculature is discussed.
Centre for Women's Health Research, Department of Obstetrics and Gynaecology, Monash University, 246 Clayton Rd, Clayton, Victoria 3168, Australia. gareth.weston@med.monash.edu.au
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D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics; D016207:Cytokines / Q000235:genetics; D016228:Endothelial Growth Factors / Q000494:pharmacology / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006133:Growth Substances / Q000235:genetics; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology / Q000502:physiology*; D008222:Lymphokines / Q000494:pharmacology / Q000502:physiology*; D009215:Myometrium / Q000187:drug effects / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D018045:Receptors, Oxytocin / Q000235:genetics; D015238:Sulfotransferases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D019208:Brain-Derived Neurotrophic Factor; D016207:Cytokines; D016228:Endothelial Growth Factors; D016326:Extracellular Matrix Proteins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D018045:Receptors, Oxytocin; D014157:Transcription Factors; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; C019277:brain-derived growth factor; D015238:Sulfotransferases; C020122:estrone sulfotransferase