Monoclonal antibodies to gonadotropin-releasing hormone (GnRH) inhibit binding of the hormone to its receptor.
Monoclonal antibodies (MAbs) specific to gonadotropin-releasing hormone (GnRH) were obtained using different strategies of conjugation of the peptide to carrier protein and immunization. Of several antibodies obtained, two, namely F1D3C5 and E2D2 bound GnRH in solution phase. Though the epitopes corresponding to the two overlapped, there was a one amino acid shift in the core epitope. These two antibodies were characterized with respect to inhibition of GnRH induced responses in rat pituitary cultures and alpha-T3.1 mouse gonadotrope cell line.
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Pradnya
P
Gangatirkar
Sunil
S
Gangadharan
Anita
A
Narendranath
Sushma
S
Nagpal
Dinakar M
DM
Salunke
Anjali A
AA
Karande
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002478:Cells, Cultured; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000235:genetics; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D006825:Hybridomas / Q000276:immunology; D007986:Luteinizing Hormone / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010902:Pituitary Gland / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D011966:Receptors, LHRH / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D016760:Proto-Oncogene Proteins c-fos; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone