Basic fibroblast growth factor, glutamine synthetase, and interleukin-6 in vitreous fluid from eyes with retinal detachment complicated by proliferative vitreoretinopathy.
OBJECTIVE
To investigate the presence of basic fibroblast growth factor (bFGF), glutamine synthetase (GS), and interleukin-6 (IL-6) in vitreous fluid from eyes with retinal detachment complicated by proliferative vitreoretinopathy (PVR).


METHODS
Comparative case series; experimental study.


METHODS
In a prospective study, we measured bFGF, GS, IL-6, and total protein in vitreous fluid samples from 53 eyes from 53 consecutive patients with PVR operated on in our hospital. As controls, vitreous fluid samples from eyes with a macular hole (n = 9) or pucker (n = 11) were used.


METHODS
Laboratory data of the patient group were compared with the control group and correlated with various clinical data, especially with visual recovery and redetachment.


RESULTS
For IL-6, bFGF, and total protein we found significantly higher levels in PVR patients' eyes than in control eyes (P =.03, P =.046, and P <.0001, respectively). Within the PVR group, no significant correlation was found for IL-6, bFGF, GS, or total protein with the various tested clinical variables.


CONCLUSIONS
We found increased levels of IL-6, bFGF, and total protein in vitreous fluid from patients' eyes with PVR. Whether the increased levels of IL-6, bFGF, and total protein are the result of an injury-induced upregulation of these proteins as part of a self-protective mechanism of the retina to minimize photoreceptor damage after the mechanical injury induced by retinal detachment is, at present, not known.
Department of Ophthalmology, University Hospital Maastricht, The Netherlands.
Ellen C
EC
La Heij
Marjo P H
MP
van de Waarenburg
Harriet G T
HG
Blaauwgeers
Alfons G H
AG
Kessels
Albert T A
AT
Liem
Charlotte
C
Theunissen
Harry
H
Steinbusch
Fred
F
Hendrikse
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012163:Retinal Detachment / Q000209:etiology / Q000378:metabolism* / Q000601:surgery; D014821:Vitrectomy; D018630:Vitreoretinopathy, Proliferative / Q000150:complications / Q000378:metabolism* / Q000601:surgery; D014822:Vitreous Body / Q000378:metabolism*
D005136:Eye Proteins; D015850:Interleukin-6; D016222:Fibroblast Growth Factor 2; D005974:Glutamate-Ammonia Ligase