A microfabrication-based dynamic array cytometer.
We have developed a microfabricated device for use in parallel luminescent single-cell assays that can sort populations upon the basis of dynamic functional responses to stimuli. This device is composed of a regular array of noncontact single-cell traps. These traps use dielectrophoresis to stably confine cells and hold them against disrupting fluid flows. Using quantitative modeling, we have designed traps with a novel asymmetric extruded-quadrupole geometry. This new trap can be physically arrayed and electrically addressed, enabling our cytometer. Situating an array of these traps in a microchannel, we have introduced cells into the array and demonstrated observation of fluorescent dynamic responses followed by sorting. Such a device has potential for use in investigating functional processes, as revealed by temporal behavior, in large numbers of single cells.
Voldman
Joel
J
Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge 02139, USA. voldman@mit.edu
Gray
Martha L
ML
Toner
Mehmet
M
Schmidt
Martin A
MA
D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D003584:Cytological Techniques / Q000295:instrumentation*; D004566:Electrodes; D018922:HL-60 Cells; D006801:Humans; D008904:Miniaturization