Use of MALDI-TOF mass spectrometry to monitor solid-phase synthesis of oligonucleotides.
MALDI-TOF mass spectrometry has been used to characterize solid-supported oligonucleotides containing natural and non-natural and non-nucleoside moieties and a variety of internucleosidic linkages including phosphate and phosphite triesters and H-phosphonate diesters. This technique was used to follow the reactions involved in oligonucleotide synthesis; this enabled direct control of the elongation and optimization of the coupling process.
Laboratoire de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-UM II, Université Montpellier II, Place E. Bataillon, France.
T
T
Guerlavais
A
A
Meyer
F
F
Debart
J-L
JL
Imbach
F
F
Morvan
J-J
JJ
Vasseur
D001483:Base Sequence; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D009838:Oligodeoxyribonucleotides