[Generation of proton motive force (Delta mu H+) in Neisseria gonorrhoeae cells].
The value of the proton motive force in the gonococci cells under incubation medium pH changing from 5 to 8 was equal to 183-192 mB. The membrane potential changed in the limits from 103 to 145 mB, while the hydrogen ions concentration gradient (delta pH) from 47 to 90 mB. The character of phenyldicarbaundecaborane absorption by the N. gonorrhoeae vesicules displays the presence of two membrane potential generators presence: respiratory chain and H(+)-ATPase. It is shown, that the inhibitors of the energy processes KCN, DCCD, CCF cause the suppression of proton motive force generators and membrane potential dissipation It is marked, that in the gonococci strains resistant to antibiotics the membrane potential is higher, than at the sensitive ones.
Dnepropetrovsk National University, Scientific-Research Institute of Biology, Ukraine. vinnikov@ff.dsu.dp.ua
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D001896:Boron Compounds / Q000378:metabolism; D004579:Electron Transport; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials; D008826:Microbial Sensitivity Tests; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D006180:Proton-Translocating ATPases / Q000378:metabolism
D001896:Boron Compounds; C100079:phenyldicarbaundecaborane; D006180:Proton-Translocating ATPases