Differences in growth and nitrogen productivity between a stay-green genotype and a wild-type of Lolium perenne under limiting relative addition rates of nitrate supply.
The stay-green mutation of the nuclear gene sid inhibits chlorophyll degradation during leaf senescence in grasses. Decreased productivity is expected under conditions of limited external N availability, due to the higher retention of N in senescent tissues. However, this has not been reported when plants are grown at limiting low external concentrations of N. In the present study a different approach was taken, based on the relative addition rate method for defining limiting N supply. Wild-type and stay-green genotypes of Lolium perenne L. were grown for 30 days in flowing solution culture and then supplied with NO3- on an hourly basis over 35 days at relative addition rates (RARs) of 0.03, 0.06, 0.09 and 0.12 day-1, ranging from severe N limitation to optimal supply. Plants were steady-state labelled with 15NO3- prior to RAR treatments, then switched to 14NO3- to allow measurement of the re-distribution of N absorbed prior to RAR control. Following acclimation, relative growth rates (RGRs) approached the corresponding RAR, but were significantly lower for stay-green than wild-type at RARs of 0.03 and 0.06 day-1. Tiller numbers were lower in stay-green plants after 35 days at all RARs except 0.12 day-1. Concentrations of total N in senescent laminae of stay-green plants exceeded those in wild-type plants by a similar margin (4.8-6.8 mg g-1 DW) irrespective of RAR. Maximum nitrogen productivity (Pn) was 3.9 g DW g-1 N day-1 (Nmin = 7.1 mg g-1 DW) in wild-type plants, and 5.1 g DW g-1 N day-1 (Nmin = 10.7 mg g-1 DW) in stay-green plants. The higher N productivity of stay-green plants indicated these plants used a smaller pool of metabolically available N more efficiently in biosynthesis compared with wild-type plants. The retention of N, absorbed prior to RAR treatments, in senescent laminae was significantly higher in stay-green plants at RAR of 0.03 day-1 after day 21 (i.e. 20% versus 15% of the total N recovered). However, in terms of the whole N economy of the plant the margin represented only 1.7% of the total N content on day 35.
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion, SY23 3EB, UK.
James
J
Macduff
Neil
N
Raistrick
Mervyn
M
Humphreys