Differential expression of Musashi1 and nestin in the adult rat hippocampus after ischemia.
Both nestin and the neural RNA-binding protein Musashi1 (Msi1) are expressed in neural stem cells in the subventricular zone. Neurogenesis in the hippocampus has received much attention, so we evaluated the expression of Msi1 and nestin in the adult rat hippocampus after transient forebrain ischemia. Both Msi1 and nestin were induced in the reactive astrocytes after ischemia, especially in the CA1 region, until 35 days after ischemia. Induction of both molecules suggested that reactive astrocytes might have immature characteristics. In the subgranular zone (SGZ) of the hippocampal dentate gyrus, Msi1-positive cells formed clusters after ischemia. These cells were labeled by bromodeoxyuridine (BrdU) but did not express glial fibrillary acidic protein. In contrast, very few nestin-positive cells were labeled by BrdU. Our results suggest that neuronal progenitor cells in the SGZ expressed Msi1 but not nestin.
Division of Strokology, Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Suita City, Osaka, Japan.
Yoshiki
Y
Yagita
Kazuo
K
Kitagawa
Tsutomu
T
Sasaki
Takaki
T
Miyata
Hideyuki
H
Okano
Masatsugu
M
Hori
Masayasu
M
Matsumoto
D000367:Age Factors; D000818:Animals; D000963:Antimetabolites; D015415:Biomarkers; D002545:Brain Ischemia / Q000378:metabolism*; D001973:Bromodeoxyuridine; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism*; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis*; D064231:Nestin; D016601:RNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
D000963:Antimetabolites; D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; C491296:Msi1 protein, rat; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D016601:RNA-Binding Proteins; D001973:Bromodeoxyuridine