Protein carbonyl content in erythrocyte membranes in type 2 diabetic patients.
Protein carbonyl groups result from free radical-induced protein oxidation; their level in tissues and plasma is a relatively stable marker of oxidative damage. Protein carbonyl contents in erythrocyte membranes were investigated in the type 2 diabetic patients with good (n = 16) and poor (n = 30) glycemic control. Diabetic patients were classified as patients with (n = 20) and without (n = 26) angiopathy. Protein carbonyl content was evaluated using the 2,4-dinitro-phenyl-hydrazine method. Protein carbonyl content and GHb levels were significantly higher in both patients with poor and good glycemic control than in control subjects (p < 0.001 in each case). There was a significant difference in protein carbonyl content between patients with poor and good glycemic control (p < 0.001). Diabetic patients with angiopathy had significantly higher protein carbonyl content and GHb levels than the diabetic patients without angiopathy (p < 0.001). These results suggest that impaired glycemic control is connected to protein oxidation, and protein oxidation may be related to underlying metabolic abnormalities and complications of diabetes.
Konukoğlu
D
D
Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, Turkey. dkonuk@yahoo.com
Kemerli
G D
GD
Sabuncu
T
T
Hatemi
H H
HH
D001786:Blood Glucose / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D003925:Diabetic Angiopathies / Q000473:pathology; D003930:Diabetic Retinopathy / Q000473:pathology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D006834:Hydrazines; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D013056:Spectrophotometry, Ultraviolet
D001786:Blood Glucose; D001798:Blood Proteins; D006442:Glycated Hemoglobin; D006834:Hydrazines; C446799:dinitrophenylhydrazine