Antioxidant activity of black currant anthocyanin aglycons and their glycosides measured by chemiluminescence in a neutral pH region and in human plasma.
The antioxidant activity of nine anthocyanin glycosides was measured in a neutral pH region using a chemiluminescence (CL) emission system in the presence of an H(2)O(2)-acetaldehyde system, and the intensities were found to be affected by three factors, pH value and both moieties of the aglycon and C-3 sugar. With an increase in pH from 4.0 to 9.0, the CL intensities increased from pH 5.0, reached their maxima at pH 6.0-7.0, and decreased at pH 9.0. Comparison of the intensities among the 3-glucosides with five different aglycons and the 3-glycosides with three different sugar moieties at C-3 showed that their strongest intensities were given by the delphinidin aglycon and 3-rutinosyl moiety, respectively. Monitoring of the CL intensity of human blood plasma for 8 h after oral administration of black currant anthocyanins (BCA) showed a rapid increase until 2 h, and a significant difference (P < 0.05) was recognized at 1-8 h.
Health and Bioscience Laboratories, Meiji Seika Kaisha, Ltd., 5-3-1, Chiyoda, Sakado-shi, Saitama 350-0289, Japan. hitoshi_matsumoto@meiji.co.jp
Hitoshi
H
Matsumoto
Yuko
Y
Nakamura
Masao
M
Hirayama
Yumiko
Y
Yoshiki
Kazuyoshi
K
Okubo
D000872:Anthocyanins / Q000032:analysis* / Q000097:blood; D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis* / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008163:Luminescent Measurements; D019684:Magnoliopsida / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry
D000872:Anthocyanins; D000975:Antioxidants; D006027:Glycosides; D010936:Plant Extracts