[The extraction of DNA from milligram amounts of wild mountain ginseng tissues].
OBJECTIVE
To keep wild mountain ginseng, a rare medicinal remedy, as intact as possible when extracting DNA from it, a new way to extract DNA has been explored.


METHODS
The CTAB method was improved and a simple and direct way was developed.


RESULTS
From 0.001 g of dried samples of ginseng root DNA was obtained in an amount up to 2.25 micrograms.


CONCLUSIONS
The RAPD fingerprinting generated by this DNA is as good as that by CTAB method. This new method may also be used for extraction of other precious Chinese medicinal materials.
Ma
X
X
Institute of Medicinal Plants, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094.
Wang
X
X
Cai
M
M
Sun
S
S
Xiao
P
P
D018744:DNA, Plant / Q000302:isolation & purification*; D005894:Panax / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods
D018744:DNA, Plant