Overexpression of chemokine-like factor 2 promotes the proliferation and survival of C2C12 skeletal muscle cells.
Chemokine-like factor 1 (CKLF1) is a novel cytokine first cloned from U937 cells. It contains different splicing forms and has chemotactic effects on a wide spectrum of cells both in vitro and in vivo; it can also stimulate the regeneration of skeletal muscle cells in vivo, but the mechanism remains unclear. To probe the myogenesis function of CKLF2, which is the largest isoform of CKLFs, C2C12 murine myoblasts were stably transfected with human CKLF2 eukaryotic expression vector. Compared with control vector transfected C2C12 cells, CKLF2 overexpression causes accelerated myoblast proliferation as determined by cell counting and [(3)H]TdR incorporation assays. In addition, CKLF2 overexpression also promotes cell differentiation, which was determined by higher expression levels of myogenin, creatine kinase, myosin and the accelerated myoblast fusion. Further analysis also indicates that CKLF2 could activate the transcription activity of the bHLH/MyoD and MEF2 families. Finally, DNA synthesis and myotube formation could also be promoted by growing C2C12 cells in conditioned media from CKLF2-transfected cells. These findings strongly suggest a role for human CKLF2 in regulation of skeletal muscle myogenesis.
Laboratory of Medical Immunology, School of Basic Medical Science, Peking University, Xueyuan Road 38, Beijing 100083, China.
Donglan
D
Xia
Xianting
X
Li
Yaxin
Y
Lou
Wenling
W
Han
Peiguo
P
Ding
Yingmei
Y
Zhang
Chunhui
C
Di
Quansheng
Q
Song
Dalong
D
Ma
D000818:Animals; D024722:Cardiac Myosins / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D018925:Chemokines / Q000096:biosynthesis / Q000502:physiology*; D003402:Creatine Kinase / Q000378:metabolism; D052283:Creatine Kinase, MM Form; D004268:DNA-Binding Proteins / Q000235:genetics; D007527:Isoenzymes / Q000378:metabolism; D062792:MARVEL Domain-Containing Proteins; D064326:MEF2 Transcription Factors; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology* / Q000502:physiology; D017570:MyoD Protein / Q000235:genetics; D018006:Myogenic Regulatory Factors; D018008:Myogenin / Q000096:biosynthesis; D018994:Myosin Light Chains / Q000378:metabolism; D019899:Paracrine Communication / Q000502:physiology; D020033:Protein Isoforms / Q000096:biosynthesis / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic / Q000502:physiology
C432676:CKLF protein, human; D018925:Chemokines; D004268:DNA-Binding Proteins; D007527:Isoenzymes; D062792:MARVEL Domain-Containing Proteins; D064326:MEF2 Transcription Factors; D017570:MyoD Protein; C503801:Myog protein, mouse; D018006:Myogenic Regulatory Factors; D018008:Myogenin; D018994:Myosin Light Chains; D020033:Protein Isoforms; D014157:Transcription Factors; C107184:myosin light chain 2; D003402:Creatine Kinase; D052283:Creatine Kinase, MM Form; D024722:Cardiac Myosins