Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae.
The 2,160,267 bp genome sequence of Streptococcus agalactiae, the leading cause of bacterial sepsis, pneumonia, and meningitis in neonates in the U.S. and Europe, is predicted to encode 2,175 genes. Genome comparisons among S. agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, and the other completely sequenced genomes identified genes specific to the streptococci and to S. agalactiae. These in silico analyses, combined with comparative genome hybridization experiments between the sequenced serotype V strain 2603 V/R and 19 S. agalactiae strains from several serotypes using whole-genome microarrays, revealed the genetic heterogeneity among S. agalactiae strains, even of the same serotype, and provided insights into the evolution of virulence mechanisms.
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
Herve
H
Tettelin
Vega
V
Masignani
Michael J
MJ
Cieslewicz
Jonathan A
JA
Eisen
Scott
S
Peterson
Michael R
MR
Wessels
Ian T
IT
Paulsen
Karen E
KE
Nelson
Immaculada
I
Margarit
Timothy D
TD
Read
Lawrence C
LC
Madoff
Alex M
AM
Wolf
Maureen J
MJ
Beanan
Lauren M
LM
Brinkac
Sean C
SC
Daugherty
Robert T
RT
DeBoy
A Scott
AS
Durkin
James F
JF
Kolonay
Ramana
R
Madupu
Matthew R
MR
Lewis
Diana
D
Radune
Nadezhda B
NB
Fedorova
David
D
Scanlan
Hoda
H
Khouri
Stephanie
S
Mulligan
Heather A
HA
Carty
Robin T
RT
Cline
Susan E
SE
Van Aken
John
J
Gill
Maria
M
Scarselli
Marirosa
M
Mora
Emilia T
ET
Iacobini
Cecilia
C
Brettoni
Giuliano
G
Galli
Massimo
M
Mariani
Filippo
F
Vegni
Domenico
D
Maione
Daniela
D
Rinaudo
Rino
R
Rappuoli
John L
JL
Telford
Dennis L
DL
Kasper
Guido
G
Grandi
Claire M
CM
Fraser
D000595:Amino Acid Sequence; D005075:Biological Evolution; D016680:Genome, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D012703:Serotyping; D013045:Species Specificity; D013290:Streptococcal Infections / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D013296:Streptococcus pneumoniae / Q000235:genetics; D013297:Streptococcus pyogenes / Q000235:genetics; D014774:Virulence / Q000235:genetics