Infection of mice with aerosolized Mycobacterium tuberculosis: use of a nose-only apparatus for delivery of low doses of inocula and design of an ultrasafe facility.
Aerosolized delivery of virulent or hypervirulent Mycobacterium tuberculosis requires careful consideration of methodology and safety. To maximize safety, we installed a nose-only aerosol apparatus that can reproducibly deliver a low dose (<100 CFU per mouse) of M. tuberculosis in a carefully designed biohazard facility.
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
J Reid
JR
Schwebach
Bing
B
Chen
Aharona
A
Glatman-Freedman
Arturo
A
Casadevall
John D
JD
McKinney
John L
JL
Harb
Patrick J
PJ
McGuire
W Emmett
WE
Barkley
Barry R
BR
Bloom
William R
WR
Jacobs
D000336:Aerosols; D000818:Animals; D003264:Containment of Biohazards; D019570:Inhalation Exposure; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009169:Mycobacterium tuberculosis; D009330:Nebulizers and Vaporizers; D012107:Research Design; D012449:Safety; D014397:Tuberculosis, Pulmonary / Q000635:transmission*
D000336:Aerosols