Onset of in vitro rhizogenesis response and peroxidase activity in Zingiber officinale (Zingiberaceae).
The induction of rooting in microshoots of Zingiber officinale cvs. Suprava, Turia local, Suruchi and V3S18 was achieved on half-strength basal Murashige and Skoog's medium supplemented with 0.5-1.0 mg/l either indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) and 2% (w/v) sucrose within 7-9 days of culture. Rooting was inhibited when the microshoots were cultured under higher concentration of auxins. The microshoots cultured on medium supplemented with NAA induced large number of thin root hairs with friable calluses within 6-7 days. Peroxidase activity was determined during root induction (0-day to the 10th day at every 2 day interval) from microshoots derived in vitro. The activity was minimum in the inductive phase (primary) and at the maximum level during the root initiative phase. These finding may be useful in monitoring the rooting behaviour in microshoots derived from different subculture and peroxidase activity as a marker for root initiation.
Plant Biotechnology Division, Plant Tissue Culture Laboratory, Regional Plant Resource Centre, Bhubaneswar-751 015, India. grrout@hotmail.com
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Rout
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Palai
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Das
D002087:Butyrates / Q000494:pharmacology; D003470:Culture Media; D020939:Zingiber officinale / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D007210:Indoleacetic Acids / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D010946:Plants, Medicinal / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development
D002087:Butyrates; D003470:Culture Media; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D009195:Peroxidase