[Polymorphic loci and polymorphism analysis of short tandem repeats within XNP gene].
To select polymorphic short tandem repeat markers within X-linked nuclear protein (XNP) gene, genomic clones which contain XNP gene were recognized by homologous analysis with XNP cDNA. By comparing the cDNA with genomic DNA, non-exonic sequences were identified, and short tandem repeats were selected from non-exonic sequences by using BCM search Launcher. Polymorphisms of the short tandem repeats in Chinese population were evaluated by PCR amplification and PAGE. Five short tandem repeats were identified from XNP gene, two of which were polymorphic. Four and 11 alleles were observed in Chinese population for XNPSTR1 and XNPSTR4, respectively. Heterozygosities were 47% for XNPSTR1 and 70% for XNPSTR4. XNPSTR1 and XNPSTR4 localized within 3' end and intron 10, respectively. Two polymorphic short tandem repeats have been identified within XNP gene and will be useful for linkage analysis and gene diagnosis of XNP gene.
Liu
Qi-Ji
QJ
Dept. of Medical Genetics, Medical School, Shandong University, Jinan 250012, China.
Gong
Yao-Qin
YQ
Guo
Chen-Hong
CH
Chen
Bing-Xi
BX
Li
Jiang-Xia
JX
Guo
Yi-Shou
YS
D002874:Chromosome Mapping; D004265:DNA Helicases; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences; D000075924:X-linked Nuclear Protein
D009687:Nuclear Proteins; D004265:DNA Helicases; C092332:ATRX protein, human; D000075924:X-linked Nuclear Protein