Diffraction-based assay for detecting multiple analytes.
The principles of diffraction are utilized to enable the simultaneous detection of multiple analytes in solution, forming the basis of a multi-analyte sensor. Probe molecules are immobilized on a substrate such that each type of molecule defines a specific pattern within the same region of substrate. The binding of a target molecule to its complementary probe is heralded by a characteristic diffraction image. This principle is demonstrated using antibody conjugates.
Goh
Betty
B
Department of Chemistry, University of Toronto, Canada M5S 3H6.
Loo
Richard W
RW
McAloney
Richard A
RA
Goh
M Cynthia
MC
D000818:Animals; D000918:Antibody Specificity; D001360:Avidin / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001710:Biotin / Q000378:metabolism; D019793:Fluorescein / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D008027:Light; D051379:Mice; D015335:Molecular Probes / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits; D012680:Sensitivity and Specificity; D012996:Solutions; D013379:Substrate Specificity
D007074:Immunoglobulin G; D015335:Molecular Probes; D012996:Solutions; D001360:Avidin; D001710:Biotin; D019793:Fluorescein