Biosensors designed for environmental and food quality control based on screen-printed graphite electrodes with different configurations.
Graphite electrodes fabricated by screen-printing have been used as amperometric detectors in biosensors based on NAD(+)-dependent dehydrogenases, tyrosinase, or genetically modified acetylcholinesterases. The mono-enzyme sensors have been optimized as disposable or reusable devices for detection of a variety of substrates important in the food industry ( D-lactic acid, L-lactic acid, acetaldehyde) or in environmental pollution control (phenols and dithiocarbamate, carbamate and organophosphorus pesticides). The sensors were prepared in four configurations differing in enzyme confinement, enzyme immobilization and location of the immobilization agent in the biosensor assembly. Tests on real samples have been performed with the biosensors; D-lactic acid and acetaldehyde have been detected in wine and phenols in air.
Centre de Phytopharmacie, Université de Perpignan, UMR CNRS 5054, 66860 Perpignan Cedex, France.
Alina
A
Avramescu
Silvana
S
Andreescu
Thierry
T
Noguer
Camelia
C
Bala
Daniel
D
Andreescu
Jean-Louis
JL
Marty
D000079:Acetaldehyde / Q000032:analysis; D000110:Acetylcholinesterase / Q000378:metabolism; D000388:Air / Q000032:analysis; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004787:Environmental Pollution; D004800:Enzymes, Immobilized / Q000378:metabolism; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010636:Phenols / Q000032:analysis; D011786:Quality Control; D011817:Rabbits; D014920:Wine / Q000032:analysis
D004800:Enzymes, Immobilized; D010636:Phenols; D019344:Lactic Acid; D006108:Graphite; D007770:L-Lactate Dehydrogenase; D014442:Monophenol Monooxygenase; D000444:Aldehyde Dehydrogenase; D000110:Acetylcholinesterase; D000079:Acetaldehyde