Silent haemoglobin variants and determination of HbA(1c) with the HPLC Bio-Rad Variant II.
OBJECTIVE
To evaluate the determination of HbA(1c) with an automated high performance liquid chromatography (HPLC) method in patients with clinically silent haemoglobin variants.


METHODS
HbA(1c) values were determined with the ion exchange HPL Bio-Rad Variant II using the high resolution beta thalassaemia programme in patients with silent haemoglobin variants, namely: Hb Graz, Hb Sherwood Forest, Hb O Padova, and Hb D.


RESULTS
All of these haemoglobin variants caused additional peaks in the chromatograms. No clinically useful HbA(1c) results were produced for patients with Hb Graz and Hb Sherwood Forest, the results for the patient with Hb D were too low, but the results for patients with Hb O Padova were acceptable.


CONCLUSIONS
The development of this automated HPLC method modification with high resolution mode aids the identification of interference caused by the described clinically silent haemoglobin variants in HbA(1c) determination.
Department of Internal Medicine, Karl-Franzens University School of Medicine, Auenbruggerplatz 15, A-8036 Graz, Austria.
T
T
Lahousen
R E
RE
Roller
R W
RW
Lipp
W J
WJ
Schnedl
D016477:Artifacts; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006442:Glycated Hemoglobin / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans
D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal; C079327:hemoglobin Graz; C032001:hemoglobin D; C079325:hemoglobin Sherwood Forest; C038278:hemoglobin O Padova