PIAS3 (protein inhibitor of activated STAT-3) modulates the transcriptional activation mediated by the nuclear receptor coactivator TIF2.
PIAS3, a member of the protein inhibitor of activated STAT family, was found to interact in vivo and in vitro with TIF2, a previously described coactivator for nuclear receptors. The interaction is mediated by two distinct non-contiguous regions of TIF2. We found that TIF2-PIAS3 interaction occurs through a unique domain of PIAS3, very rich in acidic residues and conserved throughout the PIAS family. PIAS3 modulates the ability of TIF2 to mediate ligand-enhanced transcription activation positively or negatively, for different steroid receptors. Taken together, our results indicate a potential role of PIAS3 as transcriptional modulator of TIF2-mediated signalling.
Jiménez-Lara
Ana M
AM
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Collège de France, P.O. Box 10142, 67404 Illkirch Cedex, France.
Heine
Mattias J S
MJ
Gronemeyer
Hinrich
H
D000728:Androgens / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D017931:DNA Primers; D004622:Embryo, Mammalian; D005938:Glucocorticoids / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins; D008715:Methionine / Q000378:metabolism; D051379:Mice; D016296:Mutagenesis; D009687:Nuclear Proteins / Q000378:metabolism; D051958:Nuclear Receptor Coactivator 2; D050828:Protein Inhibitors of Activated STAT; D011980:Receptors, Progesterone / Q000502:physiology; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017384:Sequence Deletion; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects / Q000502:physiology*; D014162:Transfection
D000728:Androgens; D002352:Carrier Proteins; D017931:DNA Primers; D005938:Glucocorticoids; D047908:Intracellular Signaling Peptides and Proteins; C498002:Ncoa2 protein, mouse; D009687:Nuclear Proteins; D051958:Nuclear Receptor Coactivator 2; C109279:Pias3 protein, mouse; D050828:Protein Inhibitors of Activated STAT; D011980:Receptors, Progesterone; D014157:Transcription Factors; D008715:Methionine