Simultaneous determination of selenium and antimony compounds by capillary electrophoresis with indirect fluorescence detection.
Capillary electrophoresis (CE) with indirect fluorescence detection was used to analyze selenium (selenite, selenate, selenomethionine, and selenocystine) and antimony (antimonite and antimonate) compounds. The separation was achieved by CE in 6 min with a 1.2 mM fluorescein solution at pH 9.5. Fluorescein also functioned as a background fluorophore for the indirect detection of these nonfluorescent species. Linearity of more than two orders of magnitude was generally obtained. Precision of migration times and peak areas was less than 1.0% and 7.2%, respectively. The concentration limits of detection (CLODs) was in the microM range. The detection sensitivity was generally dependent upon the transfer ratio (TR, defined as the number of moles of fluorescein ions displaced by one mole of analyte ions) of each species.
Chang
Sarah Y
SY
Department of Applied Chemistry, Chaoyang University of Technology, Wufeng, Taichung County, Taiwan. ychang@cyut.edu.tw
Chiang
Hsien-Tsung
HT
D000965:Antimony / Q000032:analysis*; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D019793:Fluorescein; D005456:Fluorescent Dyes; D009942:Organometallic Compounds / Q000032:analysis; D012643:Selenium / Q000032:analysis*; D018036:Selenium Compounds / Q000032:analysis; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D018036:Selenium Compounds; D000965:Antimony; D012643:Selenium; D019793:Fluorescein