Relative biological effectiveness of light ions in human tumoural cell lines: role of protein p53.
Protons and alpha particles of high linear energy transfer (LET) have shown an increased relative biological effectiveness (RBE) with respect to X/gamma rays for several cellular and molecular endpoints in different in vitro cell systems. To contribute to understanding the biochemical mechanisms involved in the increased effectiveness of high LET radiation, an extensive study has been designed. The present work reports the preliminary result of this study on two human tumoural cell lines, DLD1 and HCT116, (with different p53 status), which indicate that for these cell lines, p53 does not appear to take a part in the response to radiation induced DNA damage, suggesting an alternative p53-independent pathway and a cell biochemical mechanism dependent on the cell type.
INFN-Laboratori Nazionali di Legnaro, Legnaro-Padova, Italy.
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L
Baggio
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Cavinato
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R
Cherubini
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M
Conzato
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F
Cucinotta
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Favaretto
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Gerardi
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Lora
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Stoppa
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JR
Williams
D000512:Alpha Particles; D002470:Cell Survival / Q000528:radiation effects*; D015179:Colorectal Neoplasms; D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D006801:Humans; D008027:Light; D011522:Protons; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000528:radiation effects*
D011522:Protons; D016159:Tumor Suppressor Protein p53
NASA Center JSC; NASA Discipline Radiation Health; Non-NASA Center