Functional domain of bovine milk lactoferrin which inhibits the adherence of Streptococcus mutans cells to a salivary film.
The bovine lactoferrin molecule and relatively long lactoferrin fragments containing residues 473 to 538 strongly inhibited adherence of Streptococcus mutans to saliva-coated hydroxyapatite beads. Each cysteine residue in Lf411 (residues 473 to 538) was replaced by a serine residue, and the mutants Lf411-C481S and Lf411-C532S strongly inhibited S. mutans adherence. These results suggest that the functional domain of lactoferrin that binds to a salivary film lies in residues 473 to 538 and that the region might be concealed by disulfide bond formation between Cys481 and Cys532 in the Lf411 fragment.
Oho
Takahiko
T
Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan. oho@dent.kyushu-u.ac.jp
Mitoma
Morihide
M
Koga
Toshihiko
T
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002417:Cattle; D003545:Cysteine / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D017886:Durapatite; D066298:In Vitro Techniques; D007781:Lactoferrin / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D017434:Protein Structure, Tertiary; D012463:Saliva / Q000382:microbiology*; D013295:Streptococcus mutans / Q000472:pathogenicity* / Q000502:physiology*
D004269:DNA, Bacterial; D010446:Peptide Fragments; D017886:Durapatite; D007781:Lactoferrin; D003545:Cysteine