Quantitative determination of oleane derivatives in Terminalia arjuna by high performance thin layer chromatography.
A simple, precise and rapid high performance thin layer chromatographic method has been developed for the simultaneous quantitative determination of five oleane derivatives, namely, arjunic acid, arjunolic acid, arjungenin, arjunetin and arjunglucoside I from stem bark extract of Terminalia arjuna. The isolation and separation of these compounds was carried out on 60F254 layers eluted with chloroform:methanol (90:10), and the analytes were visualised through colour development with vanillin in concentrated sulphuric acid:ethanol. Scanning and quantification of the spots at 640 nm showed good recoveries in the range 96.40-101.7%.
Singh
D V
DV
Central Institute of Medicinal and Aromatic Plants, Lucknow 226 015, India.
Verma
R K
RK
Gupta
M M
MM
Kumar
Sushil
S
D002138:Calibration; D002855:Chromatography, Thin Layer / Q000379:methods*; D009828:Oleanolic Acid / Q000032:analysis*; D015203:Reproducibility of Results; D029766:Terminalia / Q000737:chemistry*
D009828:Oleanolic Acid