[Detection and identification of the transgenes in genetically modified plants on the example of Bt-potato].
We have developed diagnostic system for revealing 35S promoter CaMV and transgene CryIIIA in transgenic potato NL Russet Burbank and NL Superior companies Monsanto. Using the data about nucleotide sequences of 35S promoter (AF234316 GenBank) and gene CryIIIA (X70979 GenBank) oligonucleotides primers were synthesized for polymerase chain reaction (PCR). The technique of genomic DNA isolation from plant material, suitable for PCR has been optimized. Using Touchdown PCR method presence of specific transgene in a Bt-potato was shown. This method can be used in routine laboratory practice for revealing 35S promoter in genetically modified plants and for identification of specific transgene CryIIIA in the transformed Bt-plants.
Mel'nychuk
M D
MD
National Agrarian University, Kyiv.
Dubin
A V
AV
Spyrydonov
V H
VH
Mel'nychuk
S D
SD
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins; D017931:DNA Primers / Q000096:biosynthesis; D004731:Endotoxins / Q000235:genetics; D017343:Genes, Plant; D006460:Hemolysin Proteins; D009029:Mosaic Viruses / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D011198:Solanum tuberosum / Q000235:genetics*; D019076:Transgenes
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017931:DNA Primers; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis