Chromosomal FISH analysis of unfertilized human oocytes and polar bodies.
The incidence of chromosomal aneuploidy was studied in 208 unfertilized metaphase II human oocytes from an in vitro fertilization program by fluorescence in situ hybridization using probes for chromosomes 18, 21, and X. Chromosome spreads were prepared by a gradual fixation-air-drying method. We obtained analyzable results from 183 oocytes and 93 polar bodies; 167 oocytes (91%) were normal, 11 (6%) were diploid, and 5 (3%) were aneuploid. Of the five aneuploid oocytes, four involved chromosome 21, and one involved the X chromosome. In this study, oocyte aneuploidy caused by both nondisjunction of bivalent chromosomes and predivision of univalent chromosomes was observed. The aneuploidy rate (9.8%) in the oocytes from women aged >==35 years was significantly higher than that (0.7%) in those aged 23 to 34 years ( P = 0.0017).
Department of Obstetrics and Gynecology, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. naohonda@iroshima-u.ac.jp
Nao
N
Honda
Norio
N
Miharu
Tetsuaki
T
Hara
Osamu
O
Samura
Hiroshi
H
Honda
Koso
K
Ohama
D000328:Adult; D000782:Aneuploidy; D002869:Chromosome Aberrations; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D004171:Diploidy; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015994:Incidence; D007564:Japan / Q000453:epidemiology; D008423:Maternal Age; D008875:Middle Aged; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism / Q000473:pathology*; D014960:X Chromosome / Q000235:genetics*
D015342:DNA Probes; D004247:DNA