Main targets of tetraaza macrocyclic copper complex on L1210 murine leukemia cells.
Several metal complex agents have already been introduced into clinical tumor therapy and others are subject of antitumor studies. In this study we focused on the tetraaza macrocyclic copper complex (Cu(TAAB)Cl(2)). We studied the influence of the substance on cell growth, cell cycle, membrane integrity, necrosis, apotosis and glutathione level on the leukemic cell line L1210 in 1-day (22 h) and 3-day (72 h) experiments. The metal complex shows a dose-dependent antiproliferative effect, without affecting cell cycle phases. The present results confirm that copper complex can damage plasmatic membranes and trigger apoptosis, and that after treatment of leukemic cells with the copper complex, glutathione levels were increased.
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia.
I
I
Dovinová
H
H
Paulíková
P
P
Rauko
L'
L
Hunáková
E
E
Hanusovská
E
E
Tibenská
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D003300:Copper; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D005978:Glutathione / Q000378:metabolism; D007939:Leukemia L1210 / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009942:Organometallic Compounds / Q000494:pharmacology*; D018384:Oxidative Stress; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D009942:Organometallic Compounds; C042450:copper tetrabenzo(b,f,j,n)-1,5,9,13-tetraazacyclohexadecine; D003300:Copper; D005978:Glutathione