[Effect of human serum on the growth of Helicobacter pylori].
To investigate whether sheep blood could be replaced with human serum or fetal calf serum for Helicobacter pylori(Hp) culture, Hp was inoculated onto the culture plates containing 7% of sheep blood, human serum, or fetal calf serum respectively. The colonies were counted after 5 days of culture. Similar culture results were obtained in the plates containing sheep blood, human serum, or fetal calf serum respectively. No effect of anti-Hp-IgG antibody in human serum on the growth of Hp was found (P > 0.05). However, heat-inactivated human serum significantly suppressed the growth of Hp (P < 0.01). Human serum or fetal calf serum may be used for Hp culture where sheep blood is not available. Bactericidal activity against Hp in human serum is mediated by complements.
Fan
X G
XG
Department of Infectious Diseases, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Li
T G
TG
Harry
H X
HX
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002417:Cattle; D003470:Culture Media; D016480:Helicobacter pylori / Q000254:growth & development* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D010949:Plasma; D012756:Sheep
D000907:Antibodies, Bacterial; D003470:Culture Media; D007074:Immunoglobulin G