[Preparation of oral microspheres carrying V. cholera vaccine and its target's distribution].
To prepare oral biodegradable microspheres carrying V. cholera vaccine, the major outer membrane protein (OMP, MW = 41 kd) as a common antigen of cholera Vibriae was obtained from the classical strain Inaba 569 B, and the OMP was encapsulated in the biodegradable delivery system comprising Poly (DL-Lactide)-Co-Poly(ethylene glycol)microspheres. The average size of the microspheres was less than 5 microns, the amount of OMP encapsulated in microspheres was 15.3%. It was found that microspheres were taken up in Peyer's patches and then distributed in spleen, liver and mesenteric lymph nodes after oral administration.
Zhang
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W
Department of Microbiology, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Jia
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W
Liu
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C
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D022121:Cholera Vaccines / Q000276:immunology / Q000493:pharmacokinetics*; D004337:Drug Carriers; D051379:Mice; D008863:Microspheres; D011817:Rabbits; D014018:Tissue Distribution; D014734:Vibrio cholerae / Q000737:chemistry
D001425:Bacterial Outer Membrane Proteins; D022121:Cholera Vaccines; D004337:Drug Carriers