The kappa-opioid agonist U-69593 affects intracellular calcium level in R1.1 mouse thymoma cell line.
In this study, the effect of the kappa-opioid agonist U-69593 on the intracellular calcium level in R1.1. cells was investigated using FURA 2-AM dye. In the previous study, calcium transport into R1.1 cells was not affected by the kappa-opioid agonist (-)U50,488 [Int. J. Immunopharmacol. 21 (1999) 133]. In this study, the kappa-opioid agonist U-69593 (10(-10)-10(-6) M), decreased intracellular calcium level in unstimulated cells. This decrease could not be reversed by the kappa-opioid antagonist NBI (10(-5) or 10(-6) M). Ionophore A23187 was used to increase intracellular calcium level. Stimulation of intracellular calcium level by Ionophore A23187 was potentiated by the kappa-opioid agonist U-69593. Thus, we have shown that basal intracellular calcium level was decreased in R1.1 by the kappa-opioid agonist U-69593 and increased in R1.1 cells stimulated by Ionophore A23187.
Division of Molecular Medicine, Ruder Bosković Institute, Zagreb 10002, Croatia. kleiner@rudjer.irb.hr
Irena
I
Martin-Kleiner
Jelka
J
Gabrilovac
D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D044482:Benzeneacetamides; D002118:Calcium / Q000378:metabolism*; D007424:Intracellular Fluid / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D011759:Pyrrolidines / Q000494:pharmacology*; D017473:Receptors, Opioid, kappa / Q000819:agonists* / Q000037:antagonists & inhibitors / Q000378:metabolism; D013945:Thymoma / Q000378:metabolism*; D013953:Thymus Neoplasms / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000701:Analgesics, Opioid; D044482:Benzeneacetamides; D011759:Pyrrolidines; D017473:Receptors, Opioid, kappa; C045444:U 69593; D002118:Calcium