[The experimental study on antisense TGF-beta 1 in inhibiting scar formation in III degree burn wound].
OBJECTIVE
To investigate the effect of antisense TGF-beta 1 in inhibiting scar formation during wound healing.


METHODS
SD rats were divided into three groups after skin burn: group one was treated with antisense TGF-beta 1 oligonucleotide; group two was treated with antisense TGF-beta 1 recombinant plasmid and the control group. In different time, RT-PCR and immunohistochemistry were used to verify the expression of TGB beta 1 mRNA and protein. Type I Collagen mRNA expression was determined by hybridization in situ. Inflammatory reaction and collagen distribution were observed pathologically.


RESULTS
In the groups received antisense ODN and recombinant plasmid, the expression of TGF-beta 1 mRNA and protein reduced during 14 days after burn. In the control group, type I collagen mRNA began to express at the second week and reached a peak at the fourth week, while the antisense groups kept low expression. The antisense group also showed mild inflammatory reaction and less synthesis of collagen.


CONCLUSIONS
Antisense TGF-beta 1 could prevent the scar formation during wound healing.
Luo
Xiaoping
X
Department of Biochemistry, Shanghai, Second Medical University, Shanghai 200025, China.
Zhao
Hanfang
H
Zhang
Youzhang
Y
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000503:physiopathology; D002921:Cicatrix / Q000517:prevention & control*; D008297:Male; D016376:Oligonucleotides, Antisense / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors*; D053773:Transforming Growth Factor beta1; D014945:Wound Healing / Q000502:physiology*
D016376:Oligonucleotides, Antisense; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1