Protein carbonyl content in erythrocyte membranes in type 2 diabetic patients.
Protein carbonyl groups result from free radical-induced protein oxidation; their level in tissues and plasma is a relatively stable marker of oxidative damage. Protein carbonyl contents in erythrocyte membranes were investigated in the type 2 diabetic patients with good (n = 16) and poor (n = 30) glycemic control. Diabetic patients were classified as patients with (n = 20) and without (n = 26) angiopathy. Protein carbonyl content was evaluated using the 2,4-dinitro-phenyl-hydrazine method. Protein carbonyl content and GHb levels were significantly higher in both patients with poor and good glycemic control than in control subjects (p < 0.001 in each case). There was a significant difference in protein carbonyl content between patients with poor and good glycemic control (p < 0.001). Diabetic patients with angiopathy had significantly higher protein carbonyl content and GHb levels than the diabetic patients without angiopathy (p < 0.001). These results suggest that impaired glycemic control is connected to protein oxidation, and protein oxidation may be related to underlying metabolic abnormalities and complications of diabetes.
Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, Turkey. dkonuk@yahoo.com
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Konukoğlu
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Kemerli
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Sabuncu
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Hatemi
D001786:Blood Glucose / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D003925:Diabetic Angiopathies / Q000473:pathology; D003930:Diabetic Retinopathy / Q000473:pathology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D006834:Hydrazines; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D013056:Spectrophotometry, Ultraviolet
D001786:Blood Glucose; D001798:Blood Proteins; D006442:Glycated Hemoglobin; D006834:Hydrazines; C446799:dinitrophenylhydrazine