Survey of human papillomavirus infection in cervical intraepithelial neoplasia in Thai women.
HPV infection is known to be associated with cervical cancer development. Precancerous lesions named cervical intraepithelial neoplasia (CIN) are divided into 3 grades, i.e., CIN-1, CIN-2, and CIN-3. Here, HPV infection determined by PCR and dot hybridization was observed in these 3 different grades of formalin-fixed paraffin embedded tissues. The HPV infection was demonstrated in 33.3 per cent of CIN-1, 36.8 per cent of CIN-2 and 75 per cent of CIN-3. Using type specific probes for HPV-6, 11, 16, 18 and 33, HPV-16 was the most prevalent type (44.44%) followed by HPV-18 (16.05%) in CIN-3. Only one HPV-18 was identified in CIN-1 while CIN-2 contained one HPV-6 and one HPV-18. Mixed infection was found in CIN-3 (12.35%). All of them had HPV-16. The cervicitis cases with normal histopathology were included as control. Only 2.7 per cent of HPV infection was shown. The relative risk of HPV infection was high in CIN-3 (OR = 107.25, 95% CI = 50.29-228.73). Our data confirm the association between high-risk HPV types and development of CIN.
Bhattarakosol
Parvapan
P
Department of Microbiology, Faculty of Medicine, Chulalongkom University, Bangkok, Thailand.
Lertworapreecha
Monthon
M
Kitkumthorn
Nakarin
N
Triratanachai
Surang
S
Niruthisard
Somchai
S
D000328:Adult; D000368:Aged; D001707:Biopsy, Needle; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D016001:Confidence Intervals; D003625:Data Collection; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D016017:Odds Ratio; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000473:pathology*; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000473:pathology; D012016:Reference Values; D012680:Sensitivity and Specificity; D013785:Thailand / Q000453:epidemiology; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000473:pathology*; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000382:microbiology / Q000473:pathology*; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000382:microbiology / Q000473:pathology*