Fibroblast growth factor 2 induces increased calvarial osteoblast proliferation and cranial suture fusion.
OBJECTIVE
Craniosynostosis has been associated with fibroblast growth factors (FGFs) and their receptors. The purpose of this study was to quantitatively determine the effect of FGF2 on rat calvarial osteoblasts and a rat cranial suture formation model.


METHODS
Fetal rat calvarial osteoblasts were cultured with and without FGF2. Cell attachment and proliferation was determined by alamar Blue dye assay and cell morphology by toluidine-blue staining. In rat calvarial organ culture, postnatal day 15 rat calvariae with dura mater were placed in serum-free media with and without FGF2. A unique quantitative analysis of suture fusion was developed by obtaining measurements of suture bridging in histological serial sections at progressive stages of fusion.


RESULTS
Attachment for cells treated with FGF2 was similar to control. In contrast, proliferation was higher for cells treated with FGF2 while maintaining an osteoblastic morphology. After 5 days in organ culture, FGF2-treated posterior frontal sutures showed a dramatic increase in fusion, compared with untreated controls. This increased fusion was maintained throughout days 7 and 10 in culture. Also, fusion was enhanced on the dural side of the suture, as is normally observed in vivo, and the normal tissue architecture was maintained.


CONCLUSIONS
These results indicate that FGF2 can promote rat osteoblast attachment and normal cell morphology as well as induce cell proliferation. In calvarial organ culture, FGF2 treatment produced an enhanced suture fusion. These results provide further support for a critical role for FGF2 in cranial suture development. These studies also present a new quantitative approach to evaluating the effect of suture-perturbing growth factors on cranial suture fusion.
Department of Pediatric Dentistry, College of Dentistry, Pediatric Dentistry, Ohio State University, Columbus, Ohio 43218-2357, USA. moursi.1@osu.edu
Amr M
AM
Moursi
Phillip L
PL
Winnard
Alissa V
AV
Winnard
John M
JM
Rubenstrunk
Mark P
MP
Mooney
D000704:Analysis of Variance; D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003393:Cranial Sutures / Q000187:drug effects*; D003398:Craniosynostoses / Q000209:etiology*; D004195:Disease Models, Animal; D004388:Dura Mater / Q000378:metabolism; D005333:Fetus; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology*; D009924:Organ Culture Techniques; D010006:Osteoblasts / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D016222:Fibroblast Growth Factor 2