A polydimethylsiloxane/glass capillary electrophoresis microchip for the analysis of biogenic amines using indirect fluorescence detection.
A polydimethylsiloxane-glass capillary microchip is fabricated for the rapid analysis of a mixture of common biogenic amines using indirect fluorescence detection. Using a running buffer of phosphate and 2-propanol, and Rhodamine 110 as a background fluorophore, both co-ionic and counter-ionic systems are explored. Studies demonstrate the separation and analysis of cations using indirect fluorescence detection for the first time in a chip-based system. Resulting electrophoretic separations are achieved within a few tens of seconds with detection limits of approximately 6 microM. The reduced sample handling and rapid separations afforded by the coupling of indirect fluorescence detection with chip-based capillary electrophoresis provide a highly efficient method for the analysis and detection of molecules not possessing a chromophore or fluorophore. Furthermore, limits of detection are on a par with reported chip-based protocols that incorporate precolumn derivatisation with fluorescence detection. The current device circumvents lengthy sample preparation stages and therefore provides an attractive alternative technique for the analysis biogenic amines.
Beard
Nigel P
NP
AstraZeneca/SmithKline Beecham Centre for Analytical Sciences, Imperial College of Science, Technology and Medicine, Department of Chemistry, South Kensington, London, UK.
de Mello
Andrew J
AJ
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis / Q000302:isolation & purification*; D004129:Dimethylpolysiloxanes; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D008904:Miniaturization; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012828:Silicones
D001679:Biogenic Amines; D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D012828:Silicones; C013830:baysilon