[Integration of DNA clones related to important economic traits of Brassica napus onto Arabidopsis genetic map].
Arbidopsis thaliana and Brassica oilcrops are closely related species in Brassicae, Cruciferae, and show highly homologous genomes. After a serial important agronomic characteristics of Brassica napus being mapped with probes from EST clones of Arabidopsis and DNA clones of rapeseed, DNA segment sequencing was performed with 25 clones which were found to be closely linked to the CMS restore gene, the boron efficiency gene (BE1), the Sclerotinia resistant QTLs and the characteristic of interspecific biomass heterosis in rapeseed respectively. Highly homologous sequences were found in Arabidopsis genome by searching the relative databases. According to the position of homologous sequences on the chromosomes of Arabidopsis, the 25 clones were integrated into the genetic map of Arabidopsis. Two markers flanking BE1 in B. napus were integrated into a narrow region at the short arm of chromosome 1 of Arabidopsis. One of EST clones, pA24, from this region was used as a probe to map B. napus and consequently been integrated in a position between the two flanking markers of BE1. This event increased the confident for one to map the important characteristics of cultured Brassica species via reciprocal comparative mapping between Arabidopsis and target Brassica crops.
Wang
Li-Xia
LX
National Key Laboratory of Cenetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
Zhao
Jian-Wei
JW
Xu
Fang-Sen
FS
Liu
Ren-Hu
RH
Meng
Jin-Ling
JL
D017360:Arabidopsis / Q000235:genetics*; D029688:Brassica napus / Q000235:genetics* / Q000254:growth & development; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D018745:Genome, Plant; D026801:Synteny
D018744:DNA, Plant