Effects of extract derived from Eriobotrya japonica on liver function improvement in rats.
Eriobotrya japonica is considered a medicinal plant, and its leaves (Eriobotrya folia) have been used to treat skin diseases, as well as to relieve inflammation, pain, coughing, and sputa. In our evaluation of the pharmacological efficacy of the seed extracts, constituents of the seeds were found to contain the unsaturated fatty acids linolenic and linoleic acids and the sterol beta-sitosterol in the 70% EtOH and the MeOH extracts. The seed extracts were orally administered to rats with dimethylnitrosamine-induced hepatopathy, and blood L-asparate aminotransferase (AST) and L-alanine aminotransferase (ALT) levels, liver retinoid level, and hydroxyproline level were measured. Liver fibrosis rates calculated after Azan-Mallory staining and evaluation of the liver function-improving effects of extracts were showed that AST, ALT, and hydroxyproline levels and liver fibrosis rates were significantly lower, and retinoid levels were significantly higher in hepatopathic rats treated with 70% EtOH and MeOH extracts of the seed than in water-treated control rats. This suggests that the positive effect on liver function of the extracts varies depending on the extracting solvent used. 70% EtOH and MeOH extract of the seeds inhibited the development of liver fibrosis in hepatopathic rats, thus exhibiting potent improvement. The unsaturated linolenic and linoleic acids and the sterol beta-sitosterol contained in these extracts may also contribute to the improvement of liver function.
Nishioka
Yutaka
Y
Department of Pharmacy, Kochi Medical School Hospital, Japan. nisiokay@kochi-ms.ac.jp
Yoshioka
Saburo
S
Kusunose
Masahiko
M
Cui
Tailin
T
Hamada
Atuhide
A
Ono
Masahide
M
Miyamura
Mituhiko
M
Kyotani
Shojiro
S
D000818:Animals; D004128:Dimethylnitrosamine / Q000633:toxicity; D031983:Eriobotrya; D008099:Liver / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds
D010936:Plant Extracts; D004128:Dimethylnitrosamine