Detection and identification of aqueous saccharides by using surface-enhanced Raman spectroscopy.
The sensitive detection and characterization of carbohydrates by means of a strategy based on surface-enhanced Raman spectroscopy is demonstrated. Spectra are obtained after injecting a small amount of saccharide solution onto a roughened silver substrate, with subsequent deposition of silver colloid. The sensitivity achieved by this two-step approach enables high-quality Raman spectra to be obtained for small amounts of aqueous saccharides (5 microL of a 10(-2) M solution) utilizing minimal laser power and small signal acquisition times (a few seconds). Spectral "fingerprints" obtained for seven structurally similar monosaccharides demonstrate clearly an effective means by which each sugar can be identified. The application to more complex analyses is demonstrated for monosaccharide mixtures and a disaccharide, whereby the SERS fingerprints aid in the determination of components.
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393, USA. mrozek@purdue.edu
Melissa F
MF
Mrozek
Michael J
MJ
Weaver
D002241:Carbohydrates / Q000032:analysis*; D012680:Sensitivity and Specificity; D012834:Silver; D012996:Solutions; D013059:Spectrum Analysis, Raman
D002241:Carbohydrates; D012996:Solutions; D012834:Silver