Expression of the AMBP gene transcript and its two protein products, alpha(1)-microglobulin and bikunin, in mouse embryogenesis.
The expression pattern of the alpha(1)-microglobulin/bikunin precursor (AMBP) gene, and its two protein products were studied in mouse embryos of 8.5-15.5 days of embryonic development by in situ hybridization and immunohistochemistry. AMBP mRNA is strongly transcribed in liver parenchyma, pancreas, and intestine epithelium. Sites of weaker expression are the vessels of the umbilical cord, the developing vertebral bodies, and kidney. The alpha(1)-microglobulin and bikunin proteins are accordingly present in developing hepatocytes, pancreas, kidney, and gut. However, additional sites of protein distribution were found that do not correlate to mRNA localization: alpha(1)-microglobulin was found in myocytes and bikunin in cardiac muscle, nervous system microvasculature, and connective tissue. Both proteins were found in brain mesenchyme and meninges. Thus, a restricted expression of the AMBP mRNA in a few organs contrasts to a widespread and unique distribution of each of the two proteins.
Instituto de Neurociencias, Universidad Miguel Hernández-CSIC, Alicante, Spain. lazarill@ibgm.uva.es
Diego
D
Sánchez
Salvador
S
Martínez
Annika
A
Lindqvist
Bo
B
Akerström
Cecilia
C
Falkenberg
D000818:Animals; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012712:Serum Globulins / Q000235:genetics*; D014018:Tissue Distribution; D014360:Trypsin Inhibitor, Kunitz Soybean
D008562:Membrane Glycoproteins; D011498:Protein Precursors; D012333:RNA, Messenger; C109148:SPINT2 protein, human; D012712:Serum Globulins; D014360:Trypsin Inhibitor, Kunitz Soybean