Comparison of red wine extract and polyphenol constituents on endothelin-1 synthesis by cultured endothelial cells.
Regular consumption of red wine reduces mortality from coronary heart disease. This observation has been attributed to the anti-thrombotic effects of ethanol and to the antioxidant properties of polyphenolic compounds present in red wine. Here we show that an extract of red wine polyphenols causes a concentration-dependent inhibition of endothelin-1 synthesis in cultured bovine aortic endothelial cells. This action was associated with modifications in phosphotyrosine staining, indicating that the active components of red wine cause specific modifications of tyrosine kinase signalling. Thus inhibition of endothelin-1 synthesis by red wine may reduce the development of atherosclerosis, and hence decrease coronary heart disease.
William Harvey Research Institute, Barts & The London School of Medicine & Dentistry, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, U.K.
Noorafza Q
NQ
Khan
Delphine M
DM
Lees
Julie A
JA
Douthwaite
Martin J
MJ
Carrier
Roger
R
Corder
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001011:Aorta; D002417:Cattle; D002478:Cells, Cultured; D003327:Coronary Disease / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology; D014920:Wine
D000975:Antioxidants; D019332:Endothelin-1; D010936:Plant Extracts