The influence of two concentrations of sodium hypochlorite on human blood: changes in haemolysis, pH and protein.
OBJECTIVE
To evaluate the in vitro effect of 2.5% and 5.0% sodium hypochlorite (NaOCl) on human blood.


METHODS
Each concentration of NaOCl was reacted with human blood for 5 min at volume ratios of 1 : 1, 1 : 6, 1 : 12, each creating changes in colour, pH and temperature. Reaction suspensions were separated by centrifugation, and absorption measurements made for separated bilirubin, Fe, and protein supernatants. Each supernatant was desalted, lyophilized, and treated by SDS polyacrylamide gel electrophoresis (SOS-PAGE).


RESULTS
Increased ratios and concentrations of NaOCl caused an increase in both pH and temperature. Protein supernatants tended to decompose on SDS-PAGE. Supernatants showed increased decolourisation with 5.0% NaOCl. Concentrations of bilirubin, Ferrum and protein in supernatants decreased with increased NaOCl concentration. NaOCl had an effect on the protein component in blood.


CONCLUSIONS
These data suggest that changes in molecular structure are due to the chemical effects of NaOCl. Protein bands tended to show low molecular weight, suggesting that haemoglobin components effect the oxidation-reduction reaction.
Department of Periodontics and Endodontics, Tsurumi University, School of Dental Medicine, Tsurumi, Yokohama, Japan.
H
H
Yamaguchi
N
N
Hosoya
K
K
Kobayashi
T
T
Yokota
T
T
Arai
J
J
Nakamura
C F
CF
Cox
D000328:Adult; D001663:Bilirubin / Q000097:blood; D001790:Blood Physiological Phenomena / Q000187:drug effects*; D001798:Blood Proteins / Q000187:drug effects*; D003116:Color; D004202:Disinfectants / Q000008:administration & dosage / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000097:blood; D008422:Materials Testing; D008970:Molecular Weight; D011489:Protein Denaturation; D012973:Sodium Hypochlorite / Q000008:administration & dosage / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors
D001798:Blood Proteins; D004202:Disinfectants; D012973:Sodium Hypochlorite; D007501:Iron; D001663:Bilirubin