Successful delivery following cryopreservation of zygotes produced by in vitro matured oocytes retrieved from a woman with polycystic ovarian syndrome-like disease: a case report.
OBJECTIVE
To estimate frozen zygotes, which developed from in vitro matured oocytes retrieved from polycystic ovarian syndrome-like disease.


METHODS
Oocyte retrieval was performed on Day 15 following withdrawal bleeding. The oocytes were incubated for 24 h in TCM-199 maturation medium supplemented with follicle fluid, E2, FSH, and hCG.


RESULTS
A total of 12 immature oocytes were collected. Seven of the 12 oocytes (58.3%) developed to the metaphase-II stage, and subsequently, all seven fertilized oocytes were frozen at the pronuclear stage. The remaining five oocytes failed to develop to the metaphase-II stage after an additional 24 h of incubation. Three of seven cryopreserved oocytes were thawed and developed to 2-8-cell cleaved stage embryos. The first pregnancy failed. However, the second frozen-thawed embryo transfer resulted in the delivery of healthy twins.


CONCLUSIONS
Successful delivery using frozen zygotes from an anovulatory woman with polycystic ovarian syndrome-like disease.
Kyono Reproduction Research Center and Lady's Clinic Kyono, Furukawa, Miyagi, Japan. info@ivf-kyono.or.jp
Koichi
K
Kyono
Noritaka
N
Fukunaga
Kosuke
K
Haigo
Setsuyo
S
Chiba
Chisako
C
Ohira
Yukiko
Y
Nakajo
Yasuhisa
Y
Araki
D000328:Adult; D015925:Cryopreservation; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D009865:Oocytes / Q000502:physiology*; D011085:Polycystic Ovary Syndrome / Q000473:pathology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic