[PCR amplification and cloning of the specific region of the gene encoding 83kd antigen from Borrelia burgdorferi].
According to the published gene sequence coding the 83 kd antigen from B31 strain of Borrelia Burgdorferi, we selected the region from 1419 bp to 1896 bp as the target sequence to design a pair of oligonucleotide primers, obtained the gene fragment by using PCR, and recombinant expression vector pBX1 by cloning the gene fragment into plasmid vector pBK-CMV. The recombinant plasmid can be made into nucleic acid probe for the classification of Borrelia burgdorferi. The gene fragment product expressed by pBX1 can be used as antigen in the serological diagnosis of Lyme disease.
Institute of Infection and Immunity, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
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D000942:Antigens, Bacterial / Q000235:genetics*; D025065:Borrelia burgdorferi / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics; D012703:Serotyping
D000942:Antigens, Bacterial; D011994:Recombinant Proteins