Quorum sensing and production of autoinducer-2 in Campylobacter spp., Escherichia coli O157:H7, and Salmonella enterica serovar Typhimurium in foods.
Autoinducer molecules are utilized by gram-negative and gram-positive bacteria to regulate density-dependent gene expression by a mechanism known as quorum sensing. PCR and DNA sequencing results showed that Campylobacter jejuni and Campylobacter coli possessed luxS, which is responsible for autoinducer-2 (AI-2) production. Using a Vibrio harveyi luminescence assay, the production of AI-2 was observed in milk, chicken broth, and brucella broth by C. coli, C. jejuni, Salmonella enterica serovar Typhimurium, and Escherichia coli O157:H7 under different conditions.
Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA.
Orla M
OM
Cloak
Barbara T
BT
Solow
Connie E
CE
Briggs
Chin-Yi
CY
Chen
Pina M
PM
Fratamico
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001681:Biological Assay; D002167:Campylobacter / Q000378:metabolism*; D013437:Carbon-Sulfur Lyases; D002645:Chickens; D004777:Environment; D019453:Escherichia coli O157 / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005506:Food Contamination; D005516:Food Microbiology; D005784:Gene Amplification; D006714:Homoserine / Q000031:analogs & derivatives* / Q000378:metabolism*; D007783:Lactones / Q000378:metabolism*; D027845:Malus; D008892:Milk / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019779:Salmonella enterica / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D007783:Lactones; C091088:N-octanoylhomoserine lactone; C457339:QseA protein, E coli; D015534:Trans-Activators; D006714:Homoserine; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria