Development of a PCR test for the identification of Staphylococcus intermedius based on the 16S rDNA sequence.
The development of a PCR assay based on the 16S ribosomal RNA gene (rDNA) sequence was carried out for the identification of Staphylococcus intermedius. Sixty-six strains of S. intermedius, 70 of Staphylococcus aureus and 2 of Staphylococcus hyicus were examined for the assay. The 16S rDNA, of which the PCR target fragment makes up 901 bp corresponding to the sequence data of the gene, was detected in all strains of S. intermedius, but it was not detected in any strains of either S. aureus or S. hyicus. These results suggest that the PCR allows a simple and precise identification of S. intermedius.
Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe-shi, Hyogo 657-8501, Japan.
Yoshihisa
Y
Wakita
Junichi
J
Kawano
Akira
A
Shimizu
Václav
V
Hajek
Emi
E
Tomisaka
Ryo
R
Yasuda
Eiko
E
Matsuo
D003030:Coagulase; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000235:genetics*
D003030:Coagulase; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S