Fibroblast adhesion and proliferation on poly(ethylene glycol) hydrogels crosslinked by hydrolyzable polyrotaxane.
Fibroblast culture was performed to evaluate cell adhesion and proliferation on poly(ethylene glycol) (PEG) hydrogels crosslinked by a hydrolyzable polyrotaxane. The polyrotaxane consisting of alpha-cyclodextrins (alpha-CDs) and PEG terminated by benzyloxycarbonyl (Z)-L-phenylalanine (L-Phe) via ester linkage was used as a multi-functional crosslinker in the PEG hydrogels. From the results of contact angle and small angle light scattering measurements, it was suggested that the surface and bulk structure of the PEG hydrogels were heterogeneous. Fibroblast adhesion and proliferation on the hydrogels was observed. The number of fibroblast adhesion on the hydrogels crosslinked by the polyrotaxane was proportional to contact angle values and correlation length, and was significantly higher than those crosslinked by alpha-CDs in spite of similar contact angle and correlation length. These findings suggest that the cells recognize the surface heterogeneity due to the polyrotaxane structure, and the number of cell adhesion and proliferation is controllable by the polyrotaxane content in feed.
Watanabe
Junji
J
School of Materials Science, Japan Advanced Institute of Science and Technology,Tatsunokuchi, Ishikawa.
Ooya
Tooru
T
Nitta
Kou-Hei
KH
Park
Ki Dong
KD
Kim
Young Ha
YH
Yui
Nobuhiko
N
D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D020100:Hydrogels; D006868:Hydrolysis; D051379:Mice; D011092:Polyethylene Glycols; D043862:Rotaxanes
D003432:Cross-Linking Reagents; D020100:Hydrogels; D043862:Rotaxanes; D011092:Polyethylene Glycols