Effects of granulocyte colony-stimulating factor (g-csf) on neutrophil phagocytosis during experimental obstructive jaundice.
BACKGROUND
Obstruction ofthe extrahepati(biliary tree produces profound depression of many components ofthe immune system. G-CSF improves diseasedfiinction fneutrophils in various conditions. In this study, we planned to investigate the changes on neutrophil phagocytosis in obstruct;ve jaundice and the effect ofG-CSF adm;nistration on thisfiinction.


METHODS
Rats were divided into 5 groups as follows: the sham group and four other groups that underwent double ligation and division of common bile duct. Two of these four groups (Group 3 and 5) received G-CSF during experiment. Neutrophil hagocytosis index was determinedforgroup2and 3 attheend ofthe 15 daysand forgroup 1, 4and 5 attheend of the21 days.


RESULTS
Neutrophil phagocytosis index significantly increased at the end ofthe 15th day after the bile duct ligation (Group 2) and significantly decreased at the end ofthe 21th day after the bile duct ligation (Group 4). Neutrophil phagocytosis index in G-CSF-treated groups was significantly increased at the end ofthe 15'h days (Group 3) and increased at the end of the 21th day (Group 5).


CONCLUSIONS
As a result, neutrophil phagocytosis index is improved if G-CSF is administered later in the course of prolonged jaundice.
Kocaeli University, Medical School, Department of Genaral Surgery, Kocaeli, Turkey.
Bekir
B
Aldemir
Zafer
Z
Canturk
Gul
G
Ilbay
Deniz
D
Sahin
Mustafa
M
Dulger
D000818:Animals; D003135:Common Bile Duct / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D041781:Jaundice, Obstructive; D009504:Neutrophils / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects
D016179:Granulocyte Colony-Stimulating Factor