Micropatterned surfaces for the control of endothelial cell behaviour.
Micropatterned materials were synthesised by photoimmobilising the sulphated hyaluronic acid, adequately functionalised with a photoreactive moiety, on glass substrates. Four different patterns (10, 25, 50 and 100 microns) were obtained. The spectroscopic and microscopic analysis of the microstructured surfaces revealed that the photoimmobilisation process was successful, demonstrating that the photomask was well reproduced on the sample surface. Analysis of endothelial cell behaviour on these micropatterned materials was performed in terms of adhesion, locomotion and orientation. Decreasing the stripe dimensions a more fusiform shape of the adhered endothelial cells was observed. At the same time the cell locomotion and orientation were increased. Furthermore, a photoimmobilisation of stripes of HyalS (10 and 100 microns) was performed on a continuous HyalS layer, in turn immobilised on glass substrate. Being excluded a different chemistry between the stripe and the substrate, the influence of topography on the behaviour of endothelia cells was thus envisaged.
Department of Chemical and Biosystems Sciences and Technologies, University of Siena, Via A. Moro, 2, 53100 Siena, Italy. barbucci@unisi.it
Rolando
R
Barbucci
Stefania
S
Lamponi
Agnese
A
Magnani
Daniela
D
Pasqui
D001672:Biocompatible Materials / Q000138:chemical synthesis; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D018914:Cells, Immobilized; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D004727:Endothelium / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D005898:Glass; D006801:Humans; D006820:Hyaluronic Acid / Q000031:analogs & derivatives / Q000138:chemical synthesis*; D008422:Materials Testing / Q000379:methods*; D036103:Nanotechnology; D010781:Photography / Q000379:methods; D013455:Sulfur / Q000737:chemistry; D013499:Surface Properties
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D013455:Sulfur; D006820:Hyaluronic Acid