Analysis of the association between apolipoprotein E polymorphism and cardiovascular risk factors in an elderly population with longevity.
OBJECTIVE
To establish the allelic and genotypic frequencies related to apolipoprotein E (ApoE) polymorphism and association of the genotypes with risk factors and cardiovascular morbidity in an elderly population with longevity.


METHODS
We analyzed 70 elderly patients aged 80 years or more who were part of the Projeto Veranópolis. We used the gene amplification technique through the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and cleavage with the restriction enzyme Hha I to identify the ApoE genotypes. The most frequent genotypes were compared considering biological variables and cardiovascular risks and morbidity.


RESULTS
The frequencies of the E2, E3, and E4 alleles were 0.05, 0.84, and 0.11, respectively, and of the genotypes were as follows: E3E3 (0.70), E3E4 (0.22), E2E3 (0.06), and E2E2 (0.02). Individuals with the E3E4 had a mean age greater than those with the E3E3. No association was observed between the genotypes and the variables analyzed, except for obesity, which was associated with the E3E3 genotype. Individuals with the E3E4 genotype had high levels of LDL-cholesterol and fibrinogen as compared with those with the E3E3 genotype.


CONCLUSIONS
The results suggest that the E4E4 genotype may be associated with early mortality. A balance between the protective or neutral factors and the cardiovascular risk factors may occur among the individuals with different genotypes, attenuating the negative effects of the E4 allele.
Faculdade de Medicina da Pontifícia Universidade Católica do Rio Grande do Sul.
Carla Helena Augustin
CH
Schwanke
Ivana Beatrice Mânica
IB
da Cruz
Ney Furhmann
NF
Leal
Rosane
R
Scheibe
Yukio
Y
Moriguchi
Emílio Hideyuki
EH
Moriguchi
D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001057:Apolipoproteins E / Q000097:blood / Q000235:genetics*; D001938:Brazil / Q000453:epidemiology; D002318:Cardiovascular Diseases / Q000097:blood / Q000235:genetics* / Q000401:mortality; D016009:Chi-Square Distribution; D002784:Cholesterol / Q000097:blood; D003430:Cross-Sectional Studies; D005838:Genotype; D006801:Humans; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors
D001057:Apolipoproteins E; D002784:Cholesterol