[Determination of columbianetin, columbianetin acetate and osthol in Angelica pubescens Maxim. f. biserrata Shan et Yuan by HPLC].
OBJECTIVE
To develop a method for the determination of columbianetin, columbianetin acetate and osthol in Angelica pubescens f. biserrata


METHODS
HPLC method, ODS column (6.0 mm x 150 mm) with a mobile phase of methanol-acetonitrile-0.025 mol.L-1 phosphoric acid (32:20:48), and wave length detection at 320 nm.


CONCLUSIONS
The method can be used for the quality control of raw medicinal materials.
Cai
Q
Q
Department of Traditional Chinese Pharmacy, Liaoning College of Traditional Chinese Medicine, Shenyang 110032.
Sha
M
M
Yang
S
S
D029969:Angelica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D011564:Furocoumarins / Q000737:chemistry / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D003374:Coumarins; D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; C046733:columbianetin; C076302:columbianetin acetate; C046627:osthol