Analysis of peptides using partial (no discharge) atmospheric pressure chemical ionization conditions with ion trap mass spectrometry.
A novel approach, based on the use of atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS) conditions, but without using corona discharge, was used to analyze peptides. The proposed method was applied to three standard peptides (bombesin, trityrosine and tyrosine-glycine-glycine) as well as peptides obtained through enzymatic digestion of two standard proteins (horse cytochrome c and horse myoglobin).
Università degli Studi di Milano, Centro Interdisciplinare Studi Bio-molecolari e Applicazioni Industriali CISI, Via Fratelli Cervi 93, 20090 Segrate, Milan, Italy. dtoycr@tin.it
Simone
S
Cristoni
Luigi Rossi
LR
Bernardi
Ida
I
Biunno
Federico
F
Guidugli
D000399:Air Pressure; D000818:Animals; D001839:Bombesin / Q000032:analysis*; D003574:Cytochrome c Group / Q000737:chemistry / Q000378:metabolism; D006736:Horses; D013058:Mass Spectrometry / Q000379:methods*; D009211:Myoglobin / Q000737:chemistry / Q000378:metabolism; D009842:Oligopeptides / Q000032:analysis*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis*
D003574:Cytochrome c Group; D009211:Myoglobin; D009842:Oligopeptides; C101095:trityrosine; C044757:tyrosyl-glycyl-glycine; D014443:Tyrosine; D001839:Bombesin