Determination of the nucleotide sequences of heat shock operon groESL and the citrate synthase gene (gltA) of Anaplasma (Ehrlichia) platys for phylogenetic and diagnostic studies.
The 1,670-bp nucleotide sequence of the heat shock operon groESL and the 1,236-bp sequence of the citrate synthase gene (gltA) of Anaplasma (Ehrlichia) platys were determined. The topology of the groEL- and gltA-based phylogenetic tree was similar to that derived from 16S rRNA gene analyses with distances. Both groESL- and gltA-based PCRs specific to A. platys were also developed based upon the alignment data.
Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, 753-8515 Yamaguchi, Japan. InokumaHisashi@aol.com
Hisashi
H
Inokuma
Kaori
K
Fujii
Masaru
M
Okuda
Takafumi
T
Onishi
Jean-Pierre
JP
Beaufils
Didier
D
Raoult
Philippe
P
Brouqui
D000709:Anaplasma / Q000235:genetics*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D018833:Chaperonins / Q000235:genetics*; D002950:Citrate (si)-Synthase / Q000235:genetics*; D004285:Dogs; D016873:Ehrlichiosis / Q000175:diagnosis*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D001426:Bacterial Proteins; C097970:GroESL protein, Bacteria; D012336:RNA, Ribosomal, 16S; D002950:Citrate (si)-Synthase; D018833:Chaperonins