Increased expression of glomerular mesangial cell 5'-nucleotidase in membranous nephropathy.
It has been demonstrated in anti-Thy1 glomerulonephritis that extracellular adenine nucleotides have a significant pro-inflammatory activity, however, glomerular ATP/ADPase, which in concert with 5'-nucleotidase converts ATP/ADP, and AMP to anti-inflammatory adenosine had an anti-inflammatory role. We have studied distribution of 5'-nucleotidase and divalent cation-activated ATPase in kidney biopsies of 15 patients with glomerulonephritis. The major finding was an overexpression of 5'-nucleotidase in the mesangium of kidney from patients with membranous nephropathy. No change in 5'-nucleotidase expression was observed in other common forms of glomerulonephritis: IgA nephropathy, mesangioproliferative and mesangiocapillary glomerulonephritis. The distribution of Mg(2+)-ATPase in investigated specimens was similar to control distribution. Results obtained in this study indicate increased mesangial expression of 5'-nucleotidase in non-proliferative form of glomerulonephritis consistent to a role of mesangial cells in inflammatory processes.
Institute of Nephrology and Hemodialysis, Faculty of Medicine, Nis, Serbia.
Vojin
V
Savić
Predrag
P
Vlahović
Vladisav
V
Stefanović
D015720:5'-Nucleotidase / Q000378:metabolism*; D001706:Biopsy; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D005920:Glomerular Mesangium / Q000201:enzymology* / Q000473:pathology; D015433:Glomerulonephritis, Membranous / Q000201:enzymology* / Q000473:pathology; D006801:Humans
D015720:5'-Nucleotidase; D017301:Ca(2+) Mg(2+)-ATPase