Chromatin damage induced by fast neutrons or UV laser radiation.
Chromatin samples from livers of Wistar rats were subjected to fast neutron irradiation in doses of 10-100 Gy or to a 248 nm excimer laser radiation, in doses of 0.5-3 MJ.m-2. The action of the radiation on chromatin was monitored by chromatin intrinsic fluorescence and fluorescence lifetimes (of bound ethidium bromide to chromatin) and by analysing fluorescence resonance energy transfer between dansyl chloride and acridine orange coupled to chromatin. For the mentioned doses of UV excimer laser radiation, the action on chromatin was more intense than in the case of fast neutrons. The same types of damage are produced by the two radiations: acidic and basic destruction of chromatin protein structure, DNA strand breaking and the increase of the distance between DNA and proteins in chromatin.
Department of Molecular Genetics, V. Babes National Institute, Bucharest, Romania.serbanradu@penet.ro
L
L
Radu
B
B
Constantinescu
D
D
Gazdaru
I
I
Mihailescu
D000818:Animals; D002843:Chromatin / Q000528:radiation effects*; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects; D005214:Fast Neutrons; D007834:Lasers; D008099:Liver / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D014466:Ultraviolet Rays
D002843:Chromatin; D004247:DNA