Free-radical damage: a possible mechanism of laryngeal aging.
We conducted a study of lipid peroxidation as a marker of age-related free-radical damage in the human larynx--the first study of its kind. A colorimetric assay for malondialdehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) was performed on extracts taken from thyroarytenoid muscle harvested from fresh cadaveric laryngeal specimens. Levels of MDA and 4-HNE were measured by spectrophotometry. Correlation studies were performed by linear regression analysis. We found that MDA levels in human thyroarytenoid muscle appeared to increase with age while 4-HNE levels showed a slight decrease with age. Our findings are consistent with those of previous studies of other organ systems and indicate that there is a need for further study of free-radical damage and the effects of aging on the human larynx and on voice production.
Department of Otolaryngology-Head and Neck Surgery, University of Iowa Hospital and Clinics, 200 Hawkins Dr., Iowa City, IA 52242, USA.
Jason
J
Diamond
Jennifer
J
Skaggs
Jose M
JM
Manaligod
D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D000447:Aldehydes / Q000032:analysis / Q000378:metabolism*; D002102:Cadaver; D046508:Culture Techniques; D005260:Female; D005609:Free Radicals / Q000009:adverse effects*; D006801:Humans; D007830:Larynx / Q000378:metabolism / Q000503:physiopathology*; D016014:Linear Models; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism*; D008875:Middle Aged; D018384:Oxidative Stress; D012680:Sensitivity and Specificity; D013053:Spectrophotometry
D000447:Aldehydes; D005609:Free Radicals; D008315:Malondialdehyde; C027576:4-hydroxy-2-nonenal