Identification of an occludin cell adhesion recognition sequence.
The molecular mechanisms by which the tight junction integral membrane protein, occludin promotes cell adhesion and establishes an endothelial monolayer permeability barrier have not been elucidated. In particular, the amino acid sequences of the occludin cell adhesion recognition (CAR) sites have not been determined. Here we demonstrate that a cyclic peptide containing the sequence LYHY, which is found in the second extracellular domain of occludins in all mammalian species, inhibits the establishment of endothelial cell barriers in vitro and in vivo. This cyclic peptide also prevents the aggregation of fibroblasts stably transfected with cDNA encoding occludin. The data suggest that the LYHY motif is an occludin CAR sequence.
Department of Surgery, Royal Victoria Hospital, Montreal, Quebec, Canada.
Orest W
OW
Blaschuk
Tadayuki
T
Oshima
Barbara J
BJ
Gour
J Matthew
JM
Symonds
Jae H
JH
Park
Christopher G
CG
Kevil
Steven D
SD
Trocha
Stephanie
S
Michaud
Naotsuka
N
Okayama
John W
JW
Elrod
J Steven
JS
Alexander
Makoto
M
Sasaki
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D002199:Capillary Permeability / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002449:Cell Aggregation; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000502:physiology*; D008833:Microcirculation / Q000187:drug effects; D062793:Occludin; D010446:Peptide Fragments / Q000494:pharmacology; D017434:Protein Structure, Tertiary / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; C568987:Ocln protein, rat; D010446:Peptide Fragments