Effects of microcin SF608 and microcystin-LR, two cyanotobacterial compounds produced by Microcystis sp., on aquatic organisms.
Effects of two cyanobacterial compounds, microcin SF608 and microcystin-LR, were investigated on different physiological parameters of two organisms, the water moss, Vesicularia dubyana, and the waterflea, Daphnia magna. Both compounds are produced by Microcystis species. Microcystin-LR is a potent inhibitor of protein phosphatases 1 and 2A, and microcin SF608 inhibits serine proteases. Other effects of microcystin-LR are well documented in the literature, but adverse effects of microcin SF608 have not been investigated as yet. This study compared the effects of both compounds on detoxication enzymes, microsomal and soluble glutathione-S-transferase (m-, sGST); oxygen stress enzymes, glutathione peroxidase (GP-X), and peroxidase (POD); photosynthetic oxygen production and chlorophyll a:chlorophyll b ratio. mGST was inhibited by both compounds in both organisms, significantly by microcin SF608, possibly indirectly by inhibition of that serine protease transforming the mGST to its active form. The sGST of D. magna was inhibited by microcin SF608, but elevated by microcystin-LR, and elevated by both compounds in V. dubyana. The GP-X in D. magna was not altered by microcin SF608, but elevated parallel to the sGST, whereas the POD in V. dubyana was decreased by both. Photosynthetic oxygen production as well as the chlorophyll a/b ratio showed typical stress reactions, a decrease of oxygen production, and an increase of chlorophyll b, caused both by microcin SF608 and by microcystin-LR. Microcin SF608 was not likely to be detoxified via conjugation to glutathione. The effects of microcin SF608 and microcystin-LR demonstrate that the impact of cyanobacteria on other organisms may not only be directly related to the presently known toxins.
Institute of Biology (Genetics), Humboldt University, Chausseestrasse 117, 10115 Berlin, Germany. cwiegand@igb-berlin.de
Claudia
C
Wiegand
Anja
A
Peuthert
Stephan
S
Pflugmacher
Shmuel
S
Carmeli
D000818:Animals; D001430:Bacteriocins / Q000633:toxicity*; D019068:Bryopsida; D003621:Daphnia; D004791:Enzyme Inhibitors / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000494:pharmacology; D008387:Marine Toxins; D052998:Microcystins; D018384:Oxidative Stress; D010456:Peptides, Cyclic / Q000633:toxicity*; D009195:Peroxidase / Q000187:drug effects / Q000494:pharmacology; D010788:Photosynthesis / Q000187:drug effects; D012697:Serine Endopeptidases / Q000187:drug effects / Q000494:pharmacology; D018675:Toxicity Tests
D001430:Bacteriocins; D004791:Enzyme Inhibitors; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C020411:microcin; D009195:Peroxidase; D005979:Glutathione Peroxidase; D012697:Serine Endopeptidases; C057862:cyanoginosin LR