Rapid methods for detection and enumeration of Campylobacter spp. in foods.
Campylobacter spp. are the most commonly reported bacterial cause of acute diarrheal disease in humans throughout the world. Traditional cultural methods for the detection and quantitation of Campylobacterspp. are slow and tedious; therefore, specific, sensitive, and rapid methods for campylobacters are needed to collect sufficient data for risk assessment and food safety policy development. We developed several rapid methods based on polymerase chain reaction (PCR), DNA hybridization, hydrophobic grid membrane filters (HGMFs), and enzyme immunoassays (EIAs). A PCR assay targeting C. jejuni, combined with a simple sample preparation procedure, detects as few as 0.3 most probable number (MPN)/mL C. jejuni in naturally contaminated chicken rinses after 20-24 h enrichment. An HGMF-EIA method using a commercial polyclonal antibody for Campylobacter detects and enumerates thermophilic Campylobacter spp. from spiked chicken rinse and milk, and naturally contaminated chicken rinses. A C. jejuni-specific probe in an HGMF-DNA hybridization protocol specifically detects and quantitates C. jejuni in food samples. A dot-blot EIA combined with an MPN procedure quantitates thermophilic campylobacters from samples that might be difficult to filter through HGMFs.
Wang
Haiyan
H
Health Canada, Bureau of Microbial Hazards, Health Products and Food Branch, Food Directorate, Ottawa, Ontario. wangha@inspection.gc.ca
D000818:Animals; D001431:Bacteriological Techniques; D002167:Campylobacter / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D002645:Chickens / Q000382:microbiology; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005516:Food Microbiology; D007124:Immunoenzyme Techniques / Q000379:methods; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D018570:Risk Assessment
D004269:DNA, Bacterial