[Continuous perfusion culture hybridoma cells for production of monoclonal antibody].
Hybridoma cells were cultured by continuous perfusion in Fibra-Cel of 5L packed-bed bioreactor for 22 days in low serum or serum-free media. The corresponded amino acids were fed and serum concentration was decreased by analyzing glucose concentration, oxygen uptake rate, secretary antibody amount and amino acids concentration in culture supernatant. Comparing with continuous perfusion culture that amino acids were not fed, antibody amount of production was increased about 2-3 times. The inoculated cell density was 2.5 x 10(5) cells/mL, while the final cell density was 8.79 x 10(8) cells/mL. Antibody production was reached 295 mg/L/d at average level, and the highest level was reached 532 mg/L/d. These results provided a primary mode of enlarge culture for monoclonal antibody industralization.
Fourth Military Medical University, National Cell Engineering Reserch Center, Xi'an 710032, China.
Li
L
Mi
Ling
L
Li
Qiang
Q
Feng
Xiao-Ling
XL
Yu
Zhi-Nan
ZN
Chen
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002455:Cell Division; D002478:Cells, Cultured; D005947:Glucose / Q000494:pharmacology; D006825:Hybridomas / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010101:Oxygen Consumption; D010477:Perfusion
D000911:Antibodies, Monoclonal; D005947:Glucose