[Preparation of oral microspheres carrying V. cholera vaccine and its target's distribution].
To prepare oral biodegradable microspheres carrying V. cholera vaccine, the major outer membrane protein (OMP, MW = 41 kd) as a common antigen of cholera Vibriae was obtained from the classical strain Inaba 569 B, and the OMP was encapsulated in the biodegradable delivery system comprising Poly (DL-Lactide)-Co-Poly(ethylene glycol)microspheres. The average size of the microspheres was less than 5 microns, the amount of OMP encapsulated in microspheres was 15.3%. It was found that microspheres were taken up in Peyer's patches and then distributed in spleen, liver and mesenteric lymph nodes after oral administration.
Department of Microbiology, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
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Zhang
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Jia
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Liu
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D022121:Cholera Vaccines / Q000276:immunology / Q000493:pharmacokinetics*; D004337:Drug Carriers; D051379:Mice; D008863:Microspheres; D011817:Rabbits; D014018:Tissue Distribution; D014734:Vibrio cholerae / Q000737:chemistry
D001425:Bacterial Outer Membrane Proteins; D022121:Cholera Vaccines; D004337:Drug Carriers