Antimycobacterial physalins from Physalis angulata L. (Solanaceae).
Crude extracts and fractions from aerial parts of Physalis angulata have been bioassayed for antimycobacterial activity. Fraction A1-29-12 containing physalins B, F and D exhibited a minimum inhibitory concentration value (MIC) against Mycobacterium tuberculosis H(37)Rv strain of 32 microg/mL. Purified physalin B and physalin D were also tested showing MIC values against Mycobacterium tuberculosis H(37)Rv strain of > 128 microg/mL and 32 microg/mL respectively, suggesting that physalin D plays a relevant role in the antimycobacterial activity displayed. Structural elucidation of both physalins D and B was based on detailed (13)C and (1)H NMR spectral analysis with the aid of 2D-correlation spectroscopy ((1)H-(1)H, COSY, HSQC and HMBC). The assignment of the (13)C chemical shift for physalin D is reported here for the first time.
Januário
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AH
Unidade de Biotecnologia Vegetal, Universidade de Ribeirão Preto - UNAERP, Brazil.
Filho
E Rodrigues
ER
Pietro
R C L R
RC
Kashima
S
S
Sato
D N
DN
França
S C
SC
D000995:Antitubercular Agents / Q000737:chemistry / Q000494:pharmacology*; D007783:Lactones / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012632:Secosteroids; D019657:Solanaceae / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry / Q000494:pharmacology*
D000995:Antitubercular Agents; D007783:Lactones; D010936:Plant Extracts; D012632:Secosteroids; D013256:Steroids; C441429:physalin D; C033270:physalin B; C075425:physalin F