[Determination of oleanolic acid and ursolic acid in spica Prunellae by derivative GC method].
OBJECTIVE
To develop a GC method to determine the oleanolic acid and ursolic acid in Spica Prunellae.


METHODS
Before GC analysis, the sample was derivatized with CH2N2 solution. The GC conditions were as follows: comumn-10% SE-30(2m x 3mm) and column temperature -270 degrees C.


RESULTS
The two constituents were well separated and had good linearity in the range of 0.0025-0.4000 mg/ml. The average recoveries and RSD of oleanolic acid were 93.53% and 3.5%, 94.18% and 3.0% respectively.


CONCLUSIONS
The method is good for determining oleanolic acid and ursolic acid in Chinese medicines.
Baoji Municipal Institute for Drug Control, Baoji 721001.
H
H
Yan
L
L
Zhao
D
D
Zhu
L
L
Ding
D002849:Chromatography, Gas / Q000379:methods; D009828:Oleanolic Acid / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D031347:Prunella / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis*; D000097245:Ursolic Acid
D009828:Oleanolic Acid; D014315:Triterpenes; D000097245:Ursolic Acid