The course of Leishmania major infection in mice lacking granzyme-mediated mechanisms.
We previously showed that T cells expressing granzyme (gzm) A are more frequent in skin lesions of susceptible mice than in those of resistant mice infected with the intracellular parasite Leishmania major. To determine the in vivo role of gzm in cutaneous leishmaniasis, we examined the course of L. major infection in gzmA-deficient mice. Despite a delay in host colonization of susceptible mice, the lack of gzmA did not influence the course of lesion development or result in a discernible alteration of the interferon-gamma and interleukin-4 production. Moreover, no differences in these parameters were observed between wild-type controls and mice deficient in gzmB or both gzmA and gzmB. These findings indicate that neither gzmA nor gzmB are critical for the development of T helper cell responses and the outcome of L. major infection.
Institute for Molecular Biology of Infectious Diseases, University of Würzburg, Germany,
Veronika
V
Eisert
Uwe
U
Münster
Markus M
MM
Simon
Heidrun
H
Moll
D000818:Animals; D053804:Granzymes; D006790:Host-Parasite Interactions / Q000502:physiology; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D018320:Leishmania major / Q000502:physiology; D016773:Leishmaniasis, Cutaneous / Q000201:enzymology* / Q000235:genetics; D008198:Lymph Nodes / Q000378:metabolism; D051379:Mice / Q000469:parasitology*; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012697:Serine Endopeptidases / Q000172:deficiency* / Q000235:genetics
D015847:Interleukin-4; D007371:Interferon-gamma; D053804:Granzymes; C509214:Gzmb protein, mouse; D012697:Serine Endopeptidases