A modified micropipette aspiration technique and its application to tether formation from human neutrophils.
Tether formation, which is mechanically characterized by its threshold force and effective viscosity, is involved in neutrophil emigration from blood circulation. Using the micropipette aspiration technique, which was improved by quantitative contact control and computerized data analysis, we extracted tethers from human neutrophils treated with IL-8, PMA, or cytochalasin D. We found that both IL-8 and PMA elevated the threshold force to about twice as large as the value for passive neutrophils. All these treatments decreased the effective viscosity dramatically (approximately 80%). With a novel method, the residual cortical tension of the cytochalasin-D-treated non-spherical neutrophils was measured to be approximately 8.8 pN/microm.
Department of Biomedical Engineering, Washington University, Saint Louis, MO 63130, USA. shao@biomed.wustl.edu
Jin-Yu
JY
Shao
Jinbin
J
Xu
D002462:Cell Membrane / Q000502:physiology; D002465:Cell Movement; D003571:Cytochalasin B / Q000494:pharmacology*; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology; D008560:Membrane Fluidity; D008863:Microspheres; D009038:Motion; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013314:Stress, Mechanical; D013396:Suction / Q000295:instrumentation* / Q000379:methods; D013500:Surface Tension; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014159:Transducers
D016209:Interleukin-8; D003571:Cytochalasin B; D013755:Tetradecanoylphorbol Acetate