[Expression of ouabain-sensitive H+,K+-ATPase catalytic subunit gene in the rat epidermis].
A comparative localization of Na+,K(+)-ATPase and ouabain-sensitive H+,K(+)-ATPase in rat skin was performed using in situ RNA hybridization and immunohistochemistry. Na+,K(+)-ATPase was predominantly detected in the basal layer of epithelium, whereas the ouabain-sensitive H+,K(+)-ATPase, in the granular and prickle cell layers. The genes of these ATPases are thus expressed in epithelial cells at different stages of their development. The hypothesis was advanced that the ouabain-sensitive H+,K(+)-ATPase is involved in maintaining the skin pH value. The probes specific to the mRNAs of the full-size alpha-subunit of the ouabain-sensitive H+,K(+)-ATPase and its truncated form were used to establish a similar distribution of both mRNA variants in skin. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2002, vol. 28, no. 4; see also http://www.maik.ru.
Romanova
L G
LG
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia.
Novoselov
S V
SV
Egorov
M V
MV
Kostina
M B
MB
Shakhparonov
M I
MI
D000818:Animals; D002384:Catalysis; D004817:Epidermis / Q000033:anatomy & histology / Q000378:metabolism*; D017506:H(+)-K(+)-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D021122:Protein Subunits; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism
D021122:Protein Subunits; D017506:H(+)-K(+)-Exchanging ATPase; D000254:Sodium-Potassium-Exchanging ATPase