Bone induction in subcutaneous tissue in rats by a newly developed DNA-coated atelocollagen and bone morphogenetic protein.
A unique biomaterial, a mixture of DNA and collagen (DNA/collagen), was developed and its efficacy as a carrier matrix for bone morphogenetic protein (BMP) was evaluated histologically. The material was prepared as a composite of DNA from salmon milt and pepsin-digested type I collagen (atelocollagen) from bovine dermis. Phase-contrast and fluorescence microscopy showed atelocollagen fibres with DNA coating. The dose-response and time-course of bone induction by BMP in DNA/collagen (5 x 10 x 1 mm) in the subcutaneous tissue was investigated in 20 male Wistar rats. The BMP/DNA/collagen induced new bone in a dose-dependent manner (0, 25, 50 or 100 micrograms of BMP). Histological examination in the time-course study showed that the BMP (100 micrograms)/DNA/collagen induced bone formation, while the DNA/collagen alone resulted in the accumulation of fibroblasts. These results indicate that the DNA/collagen is effective as a carrier matrix for BMP. It provides a cell anchorage for differentiation of osteoblasts and is absorbed as bone matures.
Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Hokkaido, Japan. murata@hoku-iryo-u.ac.jp
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M
Murata
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M
Arisue
D
D
Sato
T
T
Sasaki
T
T
Shibata
Y
Y
Kuboki
D020341:Absorbable Implants; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage*; D002417:Cattle; D002454:Cell Differentiation; D020099:Coated Materials, Biocompatible; D003094:Collagen / Q000008:administration & dosage*; D004247:DNA / Q000737:chemistry*; D062109:Dermatologic Surgical Procedures; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D016503:Drug Delivery Systems; D046911:Macromolecular Substances; D008297:Male; D008567:Membranes, Artificial; D010006:Osteoblasts; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012474:Salmon; D013997:Time Factors
D019485:Bone Morphogenetic Proteins; D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D046911:Macromolecular Substances; D008567:Membranes, Artificial; C059821:atelocollagen; D003094:Collagen; D004247:DNA