Effect of intraocular pressure on optic disc topography, electroretinography, and axonal loss in a chronic pressure-induced rat model of optic nerve damage.
OBJECTIVE
To characterize the effect of intraocular pressure (IOP) on optic disc topography, retinal function, and axonal survival in a model of IOP-induced optic nerve damage in rat.


METHODS
Hypertonic (1.75 M) saline was injected into an episcleral vein of one eye of 49 Brown Norway rats, with the fellow untreated eye serving as the control. During the 1 to 3 months of follow-up, IOP was measured twice weekly in conscious animals with a handheld tonometer, and changes in disc topography and retinal function were monitored with scanning laser tomography and electroretinography (ERG), respectively. Peak IOP elevation in the experimental eye compared with the fellow control eye (peak deltaIOP), integral of IOP elevation over time (deltaIOP integral), and days of IOP elevation were calculated. Axon counts were obtained from electron micrographs of the sectioned optic nerves.


RESULTS
Progressive cupping was found in 9 (56.3%) of 16 eyes with peak deltaIOP of more than 15 mm Hg and in none of 21 eyes with peak deltaIOP less than 15 mm Hg. A strong correlation between deltaIOP integral and progressive cupping was also found, but not with days of IOP elevation. ERG abnormalities (limited to the b-wave) were found in 11 (64.7%) of 17 eyes with peak deltaIOP of more than 15 mm Hg and in 2 (8.7%) of 23 eyes with peak deltaIOP of less than 15 mm Hg. Neither of the other IOP parameters was predictive of ERG damage. The proportion of surviving axons was negatively correlated to both deltaIOP and deltaIOP integral (P <or= 0.001). Progressive cupping was evident only with more than 55% axonal loss. Similarly, ERG results remained mostly within normal limits for low to moderate axonal loss, but were markedly abnormal with more than 70% axonal loss.


CONCLUSIONS
Structural and functional changes in this model are best correlated to peak deltaIOP and not to duration of IOP elevation, suggesting the existence of an IOP-related damage threshold.
Chauhan
Balwantray C
BC
Laboratory for Retina and Optic Nerve, Dalhousie University, Halifax, Nova Scotia, Canada. bal@is.dal.ca
Pan
Jingyi
J
Archibald
Michele L
ML
LeVatte
Terry L
TL
Kelly
Melanie E M
ME
Tremblay
François
F
D000818:Animals; D001369:Axons / Q000473:pathology*; D002470:Cell Survival; D002908:Chronic Disease; D004195:Disease Models, Animal; D004596:Electroretinography; D007429:Intraocular Pressure; D007834:Lasers; D008297:Male; D009898:Optic Disk / Q000473:pathology*; D009901:Optic Nerve Diseases / Q000175:diagnosis* / Q000503:physiopathology; D051381:Rats; D011914:Rats, Inbred BN; D012160:Retina / Q000502:physiology*; D014054:Tomography