Ceftazidime-hydrolysing CTX-M-15 extended-spectrum beta-lactamase (ESBL) in Poland.
The CTX-M-15 extended-spectrum beta-lactamase (ESBL) was recently identified in Enterobacteriaceae isolates in India, and demonstrated significant hydrolytic activity against ceftazidime, in contrast to the majority of CTX-M enzymes. CTX-M-15 differs from CTX-M-3, which is one of the most prevalent ESBLs in Poland, by only a single amino acid change (Asp-240-->Gly). Three cefotaxime- and ceftazidime-resistant Enterobacteriaceae isolates, recovered during 1998-2000 in two Polish hospitals, were found to produce CTX-M-15. Similar to those from India, the isolates contained the ISEcp1 insertion sequence located upstream of the bla(CTX-M-15) gene, which has been recently demonstrated to mobilize 3'-adjacent genes to transfer between DNA replicons. However, its different position with respect to the beta-lactamase gene indicated the independent selection of the ESBL gene in the two countries.
Baraniak
Anna
A
Sera and Vaccines Central Research Laboratory, ul. Chessmska 30/34, 00-725 Warsaw, Poland.
Fiett
Janusz
J
Hryniewicz
Waleria
W
Nordmann
Patrice
P
Gniadkowski
Marek
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D002442:Ceftazidime / Q000494:pharmacology*; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D006786:Hospitals, Urban; D006801:Humans; D008969:Molecular Sequence Data; D011044:Poland / Q000453:epidemiology; D012706:Serratia marcescens / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D002442:Ceftazidime; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases