NMR spectroscopy: a useful tool for characterisation of plant extracts, the case of supercritical CO2 arnica extract.
The efficiency of two-dimensional homonuclear (1)H-(1)H correlated spectroscopy (COSY) and two-dimensional reverse heteronuclear shift correlation spectroscopy (i.e. heteronuclear multiple quantum correlation, HMQC) in characterising the content of the constituents of innovative extracts is demonstrated. These experiments were performed directly on a supercritical carbon dioxide (CO(2)) commercial extract of arnica and were able to fully characterise the active constituents, sesquiterpenes, and other metabolites extracted with the supercritical CO(2), namely polyketides. Identification of constituents was performed by combining literature data and information obtained by 2D-NMR experiments.
Department of Pharmaceutical Sciences, University of Florence, Via G. Capponi 9, 50121 Florence, Italy. ar.bilia@unifi.it
Anna Rita
AR
Bilia
Maria Camilla
MC
Bergonzi
Giovanni
G
Mazzi
Franco Francesco
FF
Vincieri
D018650:Arnica / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid / Q000379:methods* / Q000706:statistics & numerical data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
D010936:Plant Extracts