[The effect of culture conditions on hydrophobic properties of Pseudomonas aeruginosa].
The cell surface hydrophobicity (CSH) plays an important role in a adhesion of bacteria on solid surfaces. CSH of 62 Pseudomonas aeruginosa strains isolated from humans and different animals was assessed using the ammonium sulfate salt aggregation test. Bacteria were grown for 24 h and 48 h at a room temperature (22 degrees C) and 37 degrees C on enrichment broth and agar (Biomed) and tryptic soy agar (Difco). The hydrophobic properties of the Pseudomonas aeruginosa strains were depended on the temperature, time of the culture of bacteria and the kind of media. CSH properties were most frequently expressed when the analyzed strains were cultured in enrichment broth. In a such conditions Pseudomonas aeruginosa strains were more hydrophobic when grown at 22 degrees C (94% after 24 h and 87% after 48%) than those at 37 degrees C (72% after 24 h and 71% after 48 h). Among strains cultured in tryptic soy agar at 37 degrees C, 48% after 24 h and 75% after 48 h were autoaggregating, representing very strong hydrophobic properties.
Katedra Mikrobiologii Akademii Podlaskiej w Siedlcach.
Katarzyna
K
Wolska
Sylwia
S
Pogorzelska
Elzbieta
E
Fijoł
Antoni
A
Jakubczak
Kazimierz
K
Bukowski
D000362:Agar; D000818:Animals; D003470:Culture Media; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008828:Microbiological Techniques; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000502:physiology*
D003470:Culture Media; D000362:Agar