A comparison of imaging methods for use in an array biosensor.
An array biosensor has been developed which uses an actively-cooled, charge-coupled device (CCD) imager. In an effort to save money and space, a complementary metal-oxide semiconductor (CMOS) camera and photodiode were tested as replacements for the cooled CCD imager. Different concentrations of CY5 fluorescent dye in glycerol were imaged using the three different detection systems with the same imaging optics. Signal discrimination above noise was compared for each of the three systems.
Golden
Joel P
JP
Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, Code 6900, Washington, DC 20375-5348, USA. jgolden@cbmse.nrl.navy.mil
Ligler
Frances S
FS
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003080:Cold Temperature; D003952:Diagnostic Imaging / Q000379:methods*; D004867:Equipment Design; D015200:Fluoroimmunoassay / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012666:Semiconductors; D012680:Sensitivity and Specificity; D014741:Video Recording / Q000295:instrumentation* / Q000379:methods
NASA Discipline Life Sciences Technologies; Non-NASA Center