The human cytomegalovirus ribonucleotide reductase homolog UL45 is dispensable for growth in endothelial cells, as determined by a BAC-cloned clinical isolate of human cytomegalovirus with preserved wild-type characteristics.
An endothelial cell-tropic and leukotropic human cytomegalovirus (HCMV) clinical isolate was cloned as a fusion-inducing factor X-bacterial artificial chromosome in Escherichia coli, and the ribonucleotide reductase homolog UL45 was deleted. Reconstituted virus RVFIX and RV Delta UL45 grew equally well in human fibroblasts and human endothelial cells. Thus, UL45 is dispensable for growth of HCMV in both cell types.
Hahn
Gabriele
G
Max von Pettenkofer Institut für Virologie, Ludwig-Maximilians-Universität München, 80336 Munich, Germany. ghahn@m3401.mpk.med.uni-muenchen.de
Khan
Hanna
H
Baldanti
Fausto
F
Koszinowski
Ulrich H
UH
Revello
M Grazia
MG
Gerna
Giuseppe
G
D017209:Apoptosis; D002460:Cell Line; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000201:enzymology / Q000254:growth & development*; D003586:Cytomegalovirus Infections / Q000821:virology; D004730:Endothelium, Vascular / Q000166:cytology / Q000821:virology*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000821:virology; D005434:Flow Cytometry; D017353:Gene Deletion; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology; D012264:Ribonucleotide Reductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
C088748:UL45 protein, herpes simplex virus type 1; D014764:Viral Proteins; D012264:Ribonucleotide Reductases