Differing expression of enzymes of the glyoxalase system in superficial and invasive bladder carcinomas.
This work aimed to study the activities of the glyoxalase system enzymes (glyoxalase I (GI) and glyoxalase II (GII) and their gene expression in human bladder carcinomas compared with the corresponding normal mucosa. Samples of these tissues were collected from 26 patients with superficial (SBC) or invasive bladder cancer (IBC) and used to evaluate enzyme activity and gene expression by northern blot analysis. In keeping with the electrophoretic pattern and the expression level of the respective genes, GI activity significantly increased in SBC samples, while it remained unchanged in IBC samples compared with the normal mucosa. In contrast, GII showed a higher activity in the tumour (either SBC or IBC samples) versus normal tissues. These results confirm the role of the glyoxalases in detoxifying cytotoxic methylglyoxal (MG) in bladder cancer. The differing levels of GI activity level and gene expression of GI between the SBC and IBC samples could help in their differential diagnosis.
Department of Medical and Surgical Specialities, Division of Urology, University of Perugia, Policlinico Monteluce, 06122 Perugia, Italy.
E
E
Mearini
R
R
Romani
L
L
Mearini
C
C
Antognelli
A
A
Zucchi
T
T
Baroni
M
M
Porena
V N
VN
Talesa
D000368:Aged; D000369:Aged, 80 and over; D015152:Blotting, Northern; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D015870:Gene Expression; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D013869:Thiolester Hydrolases / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000201:enzymology*; D000099301:Hydroxyacylglutathione Hydrolase
D007791:Lactoylglutathione Lyase; D009363:Neoplasm Proteins; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase