Ethanol-induced Cas tyrosine phosphorylation and Fyn kinase activation in rat brain.
BACKGROUND
Tyrosine phosphorylation signaling has been reported to be involved in regulatory mechanisms of ethanol-induced modulation of the central nervous system.


METHODS
We investigated the effect of ethanol administration on tyrosine phosphorylation signaling in rat brain and also examined the possible involvement of Fyn kinase in the process.


RESULTS
Immunoprecipitation experiments showed that Crk-associated substrate (Cas) was tyrosine-phosphorylated in response to ethanol administration. Fyn kinase was shown to be activated by ethanol administration and to phosphorylate Cas on tyrosine residue in vitro. Furthermore, Fyn kinase was co-localized with Cas in rat cerebellum and cerebral cortex.


CONCLUSIONS
Cas was tyrosine-phosphorylated in rat brain by ethanol administration, and Fyn kinase was most likely involved in the process.
Department of Legal Medicine, Osaka Medical College, Daigaku, Takatsuki, Japan.
Hajime
H
Nishio
Koichi
K
Suzuki
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000473:pathology; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D002531:Cerebellum / Q000187:drug effects / Q000473:pathology; D002540:Cerebral Cortex / Q000187:drug effects / Q000473:pathology; D003712:Dendrites / Q000187:drug effects / Q000473:pathology; D004789:Enzyme Activation / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D008297:Male; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism*; D050736:Oncogene Protein v-crk; D010766:Phosphorylation / Q000187:drug effects; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D051076:Proto-Oncogene Proteins c-fyn; D051381:Rats; D017207:Rats, Sprague-Dawley; D015682:Retroviridae Proteins, Oncogenic / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013379:Substrate Specificity; D014443:Tyrosine / Q000378:metabolism*; D018909:src Homology Domains / Q000187:drug effects*; D019061:src-Family Kinases / Q000502:physiology
D050736:Oncogene Protein v-crk; D011518:Proto-Oncogene Proteins; D015682:Retroviridae Proteins, Oncogenic; D000431:Ethanol; D014443:Tyrosine; C494961:Fyn protein, rat; D015688:Oncogene Protein pp60(v-src); D051076:Proto-Oncogene Proteins c-fyn; D019061:src-Family Kinases