Orexin signaling in recombinant neuron-like cells.
To assess the role of orexin receptor signaling in neuron-like cells, Neuro-2a murine neuroblastoma and PC12 human pheochromocytoma cells were stably transfected with human OX(1) or OX(2) receptors. Activation of both receptors strongly elevated cellular inositol phosphates and Ca(2+). A difference in the potency between orexin-A and -B was seen for OX(1), but not OX(2) receptors. Dependence of the orexin-mediated Ca(2+) response on extracellular Ca(2+) and the observed Ba(2+) influx indicate that in addition to phospholipase C, orexin receptors also may couple to similar non-voltage-gated Ca(2+) channels in neuronal cells as previously characterized in non-neuronal cells.
Department of Neuroscience, Physiology, Uppsala University, BMC, P.O. Box 572, SE-75123 Uppsala, Sweden. tomas.holmqvist@fysiologi.uu.se
Tomas
T
Holmqvist
Karl E O
KE
Akerman
Jyrki P
JP
Kukkonen
D000818:Animals; D002118:Calcium / Q000502:physiology; D002352:Carrier Proteins / Q000502:physiology*; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D009447:Neuroblastoma; D009474:Neurons; D009479:Neuropeptides / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000502:physiology; D064446:Orexin Receptors; D000068797:Orexins; D016716:PC12 Cells; D010673:Pheochromocytoma; D051381:Rats; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D002352:Carrier Proteins; C000596229:HCRT protein, human; D007295:Inositol Phosphates; D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D064446:Orexin Receptors; D000068797:Orexins; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide; D011994:Recombinant Proteins; D002118:Calcium