Investigating specific antigen/antibody binding with the atomic force microscope.
The aim of this work is to detect immune complexes without any kind of labelling of each of the immunological species, with a view to create a very sensitive biosensor. This is achieved by using the atomic force microscopy. We have proceeded by imaging the antibody (anti-rabbit IgG) or anti-rabbit IgG moieties adsorbed onto mica surface, before and after incubation of two kinds of antigens: a specific (rabbit IgG) and a non-specific one (sheep IgG). The analysis using the height histograms reveals many interesting features. We propose a general framework for interpreting these analysis, which enables the discrimination between specific and non-specific complexes.
Ouerghi
O
O
Laboratoire de Physique des Interfaces, Faculté des Science de Monastir, Monastir, Tunisia.
Touhami
A
A
Othmane
A
A
Ouada
H Ben
HB
Martelet
C
C
Fretigny
C
C
Jaffrezic-Renault
N
N
D000327:Adsorption; D000538:Aluminum Silicates / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology / Q000648:ultrastructure; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis / Q000276:immunology / Q000648:ultrastructure; D001666:Binding Sites, Antibody; D006041:Goats; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D018625:Microscopy, Atomic Force / Q000379:methods*; D012756:Sheep
D000538:Aluminum Silicates; D000906:Antibodies; D000936:Antigen-Antibody Complex; D000941:Antigens; D007074:Immunoglobulin G; C011934:mica