Infection of mice with aerosolized Mycobacterium tuberculosis: use of a nose-only apparatus for delivery of low doses of inocula and design of an ultrasafe facility.
Aerosolized delivery of virulent or hypervirulent Mycobacterium tuberculosis requires careful consideration of methodology and safety. To maximize safety, we installed a nose-only aerosol apparatus that can reproducibly deliver a low dose (<100 CFU per mouse) of M. tuberculosis in a carefully designed biohazard facility.
Schwebach
J Reid
JR
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Chen
Bing
B
Glatman-Freedman
Aharona
A
Casadevall
Arturo
A
McKinney
John D
JD
Harb
John L
JL
McGuire
Patrick J
PJ
Barkley
W Emmett
WE
Bloom
Barry R
BR
Jacobs
William R
WR
D000336:Aerosols; D000818:Animals; D003264:Containment of Biohazards; D019570:Inhalation Exposure; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009169:Mycobacterium tuberculosis; D009330:Nebulizers and Vaporizers; D012107:Research Design; D012449:Safety; D014397:Tuberculosis, Pulmonary / Q000635:transmission*
D000336:Aerosols