Genotype of 86 Toxoplasma gondii isolates associated with human congenital toxoplasmosis, and correlation with clinical findings.
To study the influence of Toxoplasma gondii genotypes on the severity of human congenital toxoplasmosis (asymptomatic, benign, or severe infection or newborn or fetal death), 8 microsatellite markers were used to analyze 86 T. gondii isolates collected from patients with congenital toxoplasmosis. Seventy-four different genotypes were detected, some identical genotypes originating probably from the same source of contamination. The 3 less polymorphic microsatellite markers associated with 6 isoenzymatic markers allowed a classification of isolates into the 3 classical types and detected atypical genotypes. Whatever the clinical findings, type II isolates were largely predominant (84.88% in the whole collection and 96.49% in 57 consecutive cases). Type I and atypical isolates were not found in asymptomatic or benign congenital toxoplasmosis. However, in 4 cases in which children were not infected despite isolation of T. gondii from placenta, only type I isolates were found.
Service de Parasitologie-Mycologie, EA 3174, Faculté de Médecine, Limoges, France.
Daniel
D
Ajzenberg
Nadine
N
Cogné
Luc
L
Paris
Marie-Hélène
MH
Bessières
Philippe
P
Thulliez
Denis
D
Filisetti
Hervé
H
Pelloux
Pierre
P
Marty
Marie-Laure
ML
Dardé
D000818:Animals; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D005313:Fetal Death / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D006801:Humans; D007231:Infant, Newborn; D018895:Microsatellite Repeats / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011247:Pregnancy; D014122:Toxoplasma / Q000145:classification / Q000235:genetics*; D014125:Toxoplasmosis, Congenital / Q000382:microbiology* / Q000473:pathology
D004269:DNA, Bacterial