Capillary electrophoresis of anthraquinones from Cassia siamea.
The separation and determination of two anthraquinones, emodin and chrysophanol, and two bianthraquinones, cassiamin A and cassiamin B, were achieved by capillary electrophoresis (CE). The running electrolyte used in this method was 0.05 M hydroxypropyl-gamma-cyclodextrin in 0.1 M borate buffer (pH 9) containing 10% acetonitrile, with an applied voltage of 20 kV. Application of this technique in the determination of the main bianthraquinones, cassiamin A and cassiamin B, of Cassia siamea is demonstrated in this paper.
Kobe Pharmaceutical University, Kobe, Japan. j-koyama@kobepharma-u.ac.jp
Junko
J
Koyama
Izumi
I
Morita
Kiyoshi
K
Tagahara
Jameelah
J
Bakari
Mohammad
M
Aqil
D000880:Anthraquinones / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002366:Cassia / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry
D000880:Anthraquinones; D010936:Plant Extracts