The isolated perfused liver. a new model using autologous blood and porcine slaughterhouse organs.
BACKGROUND
Current models of isolated and perfused livers are limited by nonphysiologic perfusates or the need for the use of high numbers of laboratory animals. The present study was performed in order to rectify these difficulties.


METHODS
To establish a new isolated perfused liver model, a perfusion circuit was developed using normothermic, autologous hemoperfusion and organs obtained from a slaughterhouse.


RESULTS
Stable organ function was maintained over 220 min. The organs displayed physiologic values for measured variables, including oxygen consumption which varied from 5.2+/-1.5 ml/min at 40 min to 5.2+/-2.4 ml/min at 220 min, and bile production (0.15-0.31 ml/min, respectively).


CONCLUSIONS
The present studies demonstrate a new approach for experimental liver perfusion by combining the optimal perfusion medium of autologous blood and slaughterhouse organs as source material.
Grosse-Siestrup
C
C
Department of Comparative Medicine and Experimental Animal Science, Charité Campus Virchow, Humboldt University, Augustenburger Platz 1, D-13353 Berlin, Germany.
Nagel
S
S
Unger
V
V
Meissler
M
M
Pfeffer
J
J
Fischer
A
A
Groneberg
D A
DA
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D006439:Hemodynamics; D006464:Hemoperfusion / Q000379:methods*; D008099:Liver / Q000473:pathology / Q000502:physiology*; D013552:Swine
D000410:Alanine Transaminase