Implantation of VX2 carcinoma into the liver of rabbits: a comparison of three direct-injection methods.
The efficacy of agarose in preventing VX2 carcinoma cell leakage was evaluated and the results were compared with two traditional methods. Forty-five rabbits were divided into 3 groups: Group 1, VX2 tumor cells were injected directly into the liver and no special procedure after removal of the needle; Group 2, the puncture site was gently compressed, using an alcoholic cotton gauze, for three minutes; Group 3, 0.2 ml of heated liquid agarose was injected to seal the aperture after injection of VX2 cells. The leakage rates were 80%, 53.3% and 6.6% for group 1, group 2 and group 3, respectively. We consider agarose is a useful material in preventing the leakage in the establishment of VX2 liver tumor models.
Lin
Wan-Yu
WY
Department of Nuclear Medicine, Taichung Veterans General Hospital, 160 Taichung Harbor Road Section 3, Taichung, Korea.
Chen
Jeonhor
J
Lin
Yungchang
Y
Han
Kunwang
K
D000818:Animals; D002277:Carcinoma / Q000473:pathology*; D004195:Disease Models, Animal; D006358:Hot Temperature; D007267:Injections; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D009366:Neoplasm Seeding; D009368:Neoplasm Transplantation / Q000379:methods*; D011817:Rabbits; D012685:Sepharose
D012685:Sepharose