[Experimental study on transplantation of acellular xeno-dermis and suspended keratinocytes].
OBJECTIVE
To investigate the outcome and histological changes of transplantation of acellular xeno-dermis combined with suspended keratinocytes.


METHODS
Forty-two nude mice with full-thickness skin defect on the back were randomly divided into 2 groups, then acellular xeno-dermisand and suspended keratinocytes were adopted to cover the skin defect in the experimental group, pure suspended keratinocytes in the control group. The area of wound healing was calculated 2, 3 and 5 weeks after transplantation, and the rates of wound contraction were also calculated, and biopsy for histological examination was performed 3, 6 and 12 weeks after transplantation.


RESULTS
Compared with the experimental group, the control group showed delayed wound healing (P < 0.05), intensive wound contraction (P < 0.05), poor durability, elasticity, and cosmetic appearances as well as disordered collagen fibers. In contrast, it was observed that the proliferation of collagen fibers was regularly organized, with no obvious acute immuno-rejection responses in the experimental group.


CONCLUSIONS
The composite transplantation of acellular xenodermis and suspended keratinocytes could promote the wound healing with a satisfactory outcome.
Burn Center, Changhai Hospital, Second Military University, Shanghai, P. R. China 200433.
Wang
W
Liu
Jing-ning
JN
Huan
Yu-lin
YL
Chen
Zhao-fan
ZF
Xia
Shi-chu
SC
Xiao
Zhi-guo
ZG
Liu
Lu
L
Wang
Su-zhen
SZ
Zhang
D000818:Animals; D020405:Dermis / Q000637:transplantation*; D006085:Graft Survival; D015603:Keratinocytes / Q000637:transplantation*; D051379:Mice; D008819:Mice, Nude; D014183:Transplantation, Heterologous; D014945:Wound Healing