The PPPY motif of human T-cell leukemia virus type 1 Gag protein is required early in the budding process.
Domains required late in the virus budding process (L domains) have been identified in the Gag proteins of a number of retroviruses. Here we show that the human T-cell leukemia virus type 1 candidate L domain motif PPPY is indeed required for virus production. Strikingly, however, mutation of this motif arrested virus particles at an earlier stage in the budding process than was seen for mutation of the L domain motifs thus far described for retroviruses. In view of the exchangeability of such domains, we propose that the retrovirus budding process may involve a continuum from bud formation to membrane fission.
Le Blanc
Isabelle
I
INSERM U332, Institut Cochin de Génétique Moléculaire. Unité d'Oncologie Virale, Institut Pasteur, Paris, France.
Prévost
Marie-Christine
MC
Dokhélar
Marie-Christine
MC
Rosenberg
Arielle R
AR
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D015683:Gene Products, gag / Q000737:chemistry* / Q000502:physiology; D015368:Human T-lymphotropic virus 1 / Q000737:chemistry* / Q000502:physiology; D006801:Humans; D008561:Membrane Fusion; D008969:Molecular Sequence Data; D014779:Virus Replication
D015683:Gene Products, gag