Long-term alterations of oral mucosa in radiotherapy patients.
OBJECTIVE
The aim of this investigation was to describe the alterations in oral mucosa after radiotherapy.


METHODS
Biopsies were taken from patients before irradiation, at 60 Gy, and 6-12 months after radiotherapy. Histomorphological evaluation of the vessels was performed, and endothelial expression of ICAM-1, VCAM-1, and E-selectin was also evaluated, as well as distribution of LFA-1-, Mac-1-, VLA-4-, RM3/1-, 27E10-, and 25F9-bearing cells in the subepithelial tissue.


RESULTS
The expression of ICAM-1 was downregulated after radiotherapy, whereas the percentage of LFA-1- and VLA-4-bearing cells increased. VCAM-1 remained at low levels. The subepithelial infiltration was still dominated by RM3/1-positive macrophages. The number of vessels decreased, while the lumina of the remaining vessels in the deeper connective layer increased.


CONCLUSIONS
The late effects of radiotherapy are characterized by a decreased number of blood vessels and by significantly different expression patterns of the adhesion molecules studied, and of integrins and macrophage subpopulations compared to the conditions before irradiation and at 60 Gy.
Department of Radiotherapy, University of Münster, Münster, Germany. prott@stahlentherapie-wiesbaden.de
Franz-Josef
FJ
Prott
Jörg
J
Handschel
Oliver
O
Micke
Cord
C
Sunderkötter
Ulrich
U
Meyer
Josef
J
Piffko
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000532:radiotherapy*; D006258:Head and Neck Neoplasms / Q000737:chemistry / Q000532:radiotherapy*; D006801:Humans; D039041:Integrin alpha4beta1; D016023:Integrins / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D008264:Macrophages / Q000528:radiation effects; D008875:Middle Aged; D009061:Mouth Mucosa / Q000737:chemistry / Q000473:pathology / Q000528:radiation effects*; D011446:Prospective Studies; D011878:Radiotherapy / Q000009:adverse effects*; D016201:Receptors, Lymphocyte Homing / Q000032:analysis; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis
D039041:Integrin alpha4beta1; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1; D016201:Receptors, Lymphocyte Homing; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1