Construction of a zebrafish cDNA microarray: gene expression profiling of the zebrafish during development.
Vertebrate embryogenesis is a complex process controlled by a transcriptional hierarchy that coordinates the action of thousands of genes. To identify and analyze the expression patterns of these genes, we constructed a zebrafish cDNA microarray containing 4512 unique genes identified from zebrafish embryonic heart, adult hearts, and skeletal muscle cDNA libraries. We examined the patterns of gene expression during development in the zebrafish between five time points relative to 12h post-fertilization (hpf). Differentially expressed genes can be grouped into two categories, early genes that are expressed at 5hpf and genes expressed at 48/72/120hpf. Furthermore, we report the utilization of cDNA microarray technology to investigate the adaptive molecular responses of zebrafish to hypoxia during development. Our study provides the first utilization of cDNA microarray in the zebrafish and reveals dynamic changes in levels of gene expression in relation to development and survival of the zebrafish embryos under hypoxic stress.
Department of Laboratory Medicine and Pathobiology, University of Toronto, M5G 1L5, Toronto, Ont., Canada.
Christopher
C
Ton
Dimitri
D
Stamatiou
Victor J
VJ
Dzau
Choong-Chin
CC
Liew
D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004625:Embryo, Nonmammalian / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D007700:Kinetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger