Detection of Cryptosporidium parvum antigen by co-agglutination test and ELISA.
Confirmation of the presence of Cryptosporidium in environmental samples is laborious, costly and often difficult. We report here a simple and economic slide agglutination test (co-agglutination test) for detecting cryptosporidial antigen in stool, serum and water. The results show that as a screening method co-agglutination is clearly superior to enzyme-linked immunosorbent assay (ELISA) and modified Ziehl-Neelsen staining, although ELISA is more accurate. The co-agglutination test is recommended for application as a new tool for detecting cryptosporidial antigen in large-scale epidemiological surveys.
Michel
M Y
MY
Faculty of Medicine, University of Alexandria, Alexandria, Egypt.
Khalifa
A M
AM
Ibrahim
I R
IR
D000208:Acute Disease; D000372:Agglutination Tests / Q000379:methods* / Q000592:standards; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000097:blood / Q000276:immunology; D001769:Blood / Q000469:parasitology; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D003457:Cryptosporidiosis / Q000097:blood / Q000150:complications / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000276:immunology*; D003967:Diarrhea / Q000469:parasitology; D004195:Disease Models, Animal; D004534:Egypt; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005243:Feces / Q000469:parasitology; D006801:Humans; D008403:Mass Screening / Q000379:methods / Q000592:standards; D051379:Mice; D012680:Sensitivity and Specificity; D014867:Water / Q000469:parasitology
D000953:Antigens, Protozoan; D014867:Water