Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum.
An erythromycin resistance plasmid, pAJ01 was isolated from Loctococcus lactis isolate C5 that was isolated from a healthy two-week-old chicken cecum. A 4 kb plasmid was transformed into plasmidless L. lactis MG1363 before a restriction endonuclease map was constructed. It was then fused with pUC19 to form pAJ02, which can replicate in Escherichia coli XLI-Blue as well as L. lactis MG1363. The plasmid was stably maintained in Lactococcus for more than 100 generations.
Raha
A R
AR
Faculty of Food Science and Biotechnology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. raha@fsb.upm.edu.my
Ross
E
E
Yusoff
K
K
Manap
M Y
MY
Ideris
A
A
D000818:Animals; D002432:Cecum / Q000382:microbiology*; D002645:Chickens; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial; D004917:Erythromycin / Q000494:pharmacology*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D013294:Lactococcus lactis / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D014169:Transformation, Bacterial
D004917:Erythromycin