An integrated CMOS microluminometer for low-level luminescence sensing in the bioluminescent bioreporter integrated circuit.
We report an integrated CMOS microluminometer for the detection of low-level bioluminescence in whole cell biosensing applications. This microluminometer is the microelectronic portion of the bioluminescent bioreporter integrated circuit (BBIC). This device uses the n-well/p-substrate junction of a standard bulk CMOS IC process to form the integrated photodetector. This photodetector uses a distributed electrode configuration that minimizes detector noise. Signal processing is accomplished with a current-to-frequency converter circuit that forms the causal portion of the matched filter for dc luminescence in wide-band white noise. Measurements show that luminescence can be detected from as few as 4 x 10(5) cells/ml.
Simpson
M L
ML
Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA. simpsonml1@ornl.gov
Sayler
G S
GS
Patterson
G
G
Nivens
D E
DE
Bolton
E K
EK
Rochelle
J M
JM
Arnott
J C
JC
Applegate
B M
BM
Ripp
S
S
Guillorn
M A
MA
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004784:Environmental Monitoring; D004867:Equipment Design; D016014:Linear Models; D008163:Luminescent Measurements; D008839:Microelectrodes; D011551:Pseudomonas fluorescens; D012815:Signal Processing, Computer-Assisted
NASA Discipline Life Sciences Technologies; Non-NASA Center