Results of crossmatch between chimpanzee lymphocytes and sera of highly sensitized potential renal transplant recipients.
BACKGROUND
We tested the hypothesis that patients with a high frequency of lymphocytotoxic antibodies against human cells are not highly sensitized to major histocompatibility complexes expressed by chimpanzee cells.


METHODS
Sera from six "hopelessly" sensitized patients (percentage reactive antibodies (PRA) > 99%) on the renal transplant waiting list were crossmatched with peripheral blood lymphocytes from 10 chimpanzees. Lymphocytotoxic antibodies reacting with chimpanzee peripheral blood lymphocytes were identified.


RESULTS
Three of the six patients with a high frequency of lymphocytotoxic antibodies against human cells had no xenospecific antibodies.


CONCLUSIONS
Patients with a high frequency of lymphocytotoxic antibodies against human cells are not all panel-reactive to chimpanzees. Unilateral donor nephrectomy in non-human primates may offer an opportunity for safe expansion of the donor organ pool for these patients.
Department of Surgery, Presbyterian Hospital of New York, Columbia-Presbyterian Medical Center, New York, NY, USA.
David G
DG
Rabkin
Roman
R
Nowygrod
Mark A
MA
Hardy
Keith
K
Reemtsma
D000818:Animals; D000910:Antibodies, Heterophile / Q000097:blood / Q000276:immunology*; D015478:Antigens, Heterophile / Q000276:immunology*; D001788:Blood Grouping and Crossmatching; D006680:HLA Antigens / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000097:blood / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology*; D016030:Kidney Transplantation / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D009392:Nephrectomy; D002679:Pan troglodytes / Q000276:immunology*; D013045:Species Specificity; D009927:Tissue and Organ Procurement
D000910:Antibodies, Heterophile; D015478:Antigens, Heterophile; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007518:Isoantibodies; D007519:Isoantigens