Development of an enzyme-linked immunosorbent assay for bisphenol a using chicken immunoglobulins.
Bisphenol A was coupled, after derivatization into a suitable hapten, to bovine serum albumin and ovalbumin in order to produce immunizing and coating antigens. The immunizing antigens were injected into chickens, which allowed the isolation of specific bisphenol A immunoglobulins from the egg yolk. These antibodies were used in an indirect competitive enzyme-linked immunosorbent assay for the determination of bisphenol A in aqueous solutions. Various parameters, influencing the assay sensitivity, were evaluated. The applicability of the assay for the determination of bisphenol A in milk was also studied. The assay was not as sensitive as other analytical techniques used in bisphenol A analysis, since typical I(50) levels of 2.5 microM were reached in aqueous solutions. This study nevertheless illustrates the usefulness and the potency of chicken antibodies in the analysis of migration residues from packaging materials using immunochemical techniques. In addition, the assay showed to be quite specific for bisphenol A as well. Only for bisphenol A analogues, cross reactivities of about 40% were reached, enabling the use of the antibodies for the screening of bisphenol A and alike compounds.
Laboratory of Food Chemistry and Analysis, Department of Food Technology and Nutrition, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. Bruno.Demeulenaer@rug.ac.be
Bruno
B
De Meulenaer
Katleen
K
Baert
Heikki
H
Lanckriet
Vera
V
Van Hoed
Andre
A
Huyghebaert
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D001559:Benzhydryl Compounds; D001667:Binding, Competitive; D002645:Chickens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006241:Haptens / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007114:Immunization; D008892:Milk / Q000737:chemistry; D009994:Osmolar Concentration; D010636:Phenols / Q000032:analysis* / Q000276:immunology; D011136:Polysorbates; D012680:Sensitivity and Specificity; D012996:Solutions; D014867:Water; D000098826:Bisphenol A Compounds
D000906:Antibodies; D000941:Antigens; D001559:Benzhydryl Compounds; D006241:Haptens; D010636:Phenols; D011136:Polysorbates; D012996:Solutions; D014867:Water; C006780:bisphenol A; D000098826:Bisphenol A Compounds