Prevalence, rate of persistence and respiratory tract symptoms of Chlamydia pneumoniae infection in 1211 kindergarten and school age children.
BACKGROUND
is a common cause of respiratory disease, but little is known about asymptomatic infection, duration of persistent respiratory tract infection and seasonal changes of prevalence in a normal large sample size pediatric population.


METHODS
We studied the prevalence of infection in 1211 children of 3 age groups: 3- to 4-year-old kindergarten children ( = 184) and schoolchildren attending first and second ( = 353) or seventh and eighth grade classes ( = 674). Polymerase chain reaction and enzyme immunoassay detection (PCR-EIA) of throat swabs were used. Respiratory tract symptoms (cough, rhinitis, earache or sore throat) were recorded in 1028 schoolchildren. Follow-up examinations in PCR-positive patients were performed until negative.


RESULTS
PCR was positive in 68 children (5.6%) without significant age and gender related differences in prevalence. Epidemics were confirmed with a prevalence up to 24% in a primary school in December and April. In schoolchildren, asymptomatic infection was a common feature, reaching 54% (32 of 59) of PCR-EIA positives. The rate of asymptomatic infection was 6% (32 of 531 schoolchildren without symptoms). Of the 32 asymptomatic PCR-EIA positives, 26 (81%) were children attending seventh and eighth grade classes. In 3 children PCR-EIA remained positive at 3 to 5 weeks and became negative during the next 7 to 9 weeks. One of 2 schoolchildren with persistent infection was asymptomatic.


CONCLUSIONS
We conclude that infection is common in the childhood population studied with seasonal variations in prevalence and epidemic-like occurrence. Asymptomatic infection occurs, especially in teenagers, but persistent infection is rare.
Department of Infectious, Bronchopulmorary and Allergic Deseases, Children's and Youth Hospital, Ernst-Moritz-Arndt University, Greifwald, Germany.
Sebastian M
SM
Schmidt
Cornelia E
CE
Muller
Bernd
B
Mahner
Siegfried K
SK
Wiersbitzky
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D023521:Chlamydophila Infections / Q000453:epidemiology* / Q000503:physiopathology*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000382:microbiology / Q000503:physiopathology*; D012574:Schools; D012621:Seasons; D013334:Students