[Effect of human serum on the growth of Helicobacter pylori].
To investigate whether sheep blood could be replaced with human serum or fetal calf serum for Helicobacter pylori(Hp) culture, Hp was inoculated onto the culture plates containing 7% of sheep blood, human serum, or fetal calf serum respectively. The colonies were counted after 5 days of culture. Similar culture results were obtained in the plates containing sheep blood, human serum, or fetal calf serum respectively. No effect of anti-Hp-IgG antibody in human serum on the growth of Hp was found (P > 0.05). However, heat-inactivated human serum significantly suppressed the growth of Hp (P < 0.01). Human serum or fetal calf serum may be used for Hp culture where sheep blood is not available. Bactericidal activity against Hp in human serum is mediated by complements.
Department of Infectious Diseases, Xiangya Hospital, Hunan Medical University, Changsha 410008.
X G
XG
Fan
T G
TG
Li
H X
HX
Harry
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002417:Cattle; D003470:Culture Media; D016480:Helicobacter pylori / Q000254:growth & development* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D010949:Plasma; D012756:Sheep
D000907:Antibodies, Bacterial; D003470:Culture Media; D007074:Immunoglobulin G