Lung-stage protein profile and antigenic relationship between Ascaris lumbricoides and Ascaris suum.
The protein profile and antigenic properties of lung-stage larvae of Ascaris lumbricoides and A. suum were studied using 2-dimensional electrophoresis and immunoblot analysis, respectively. The protein profiles of the 2 parasites were identical except for the presence of only 1 major protein spot specific for each. There was a complete cross-reactivity between the 2 parasites at the immunological level, and no specific antigen was recognized using specific antibody raised against the 2 parasites in rabbits.
Wossene
Abebe
A
Laboratory of Parasitic Diseases, National Institute of Animal Health, National Agricultural Research Organization, Tsukuba, Ibaraki 305-0856, Japan.
Tsuji
Naotoshi
N
Kasuga-Aoki
Harue
H
Miyoshi
Takeharu
T
Isobe
Takashi
T
Arakawa
Takeshi
T
Matsumoto
Yasunobu
Y
Yoshihara
Shinobu
S
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D017164:Ascaris lumbricoides / Q000737:chemistry* / Q000276:immunology; D017165:Ascaris suum / Q000737:chemistry* / Q000276:immunology; D003429:Cross Reactions; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015801:Helminth Proteins / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting; D007814:Larva / Q000737:chemistry / Q000276:immunology; D008168:Lung / Q000469:parasitology; D013045:Species Specificity; D013552:Swine
D000947:Antigens, Helminth; D015801:Helminth Proteins