Strategies for augmenting the pentachlorophenol degradation potential of UASB anaerobic granules.
Anaerobic degradation of pentachlorophenol (PCP) is an example of a process that may benefit from enrichment or bioaugmentation. In one approach, enrichment acceleration was attempted by applying an on-line control-based selective stress strategy to a native anaerobic upflow sludge bed (UASB) system; this strategy linked PCP loading rate to methane production. As a result, the reactor biomass potential for PCP complete dechlorination reached a rate of 4 mg g(-1) volatile suspended solid (VSS) day(-1) within a period of 120 days. In another approach, a pure culture, Desulfitobacterium frappieri PCP-1, a strictly anaerobic Gram-positive bacterium, was used to augment the granular biomass of the UASB reactor. This also resulted in a specific degradation rate of 4 mg PCPg(-1) VSS day(-1); however, this potential was attained within 56 days. Fluorescent in situ hybridization (FISH) showed that the PCP-1 strain was able to rapidly attach to the granule and densely colonize the outer biofilm layer.
Guiot
S R
SR
Biotechnology Research Institute, Montréal, Québec, Canada. serge.guiot@nrc.ca
Tartakovsky
B
B
Lanthier
M
M
Lévesque
M J
MJ
Manuel
M F
MF
Beaudet
R
R
Greer
C W
CW
Villemur
R
R
D001421:Bacteria, Anaerobic / Q000502:physiology*; D001673:Biodegradation, Environmental; D018441:Biofilms; D018533:Biomass; D019149:Bioreactors; D004269:DNA, Bacterial; D004785:Environmental Pollutants / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence; D010316:Particle Size; D010416:Pentachlorophenol / Q000378:metabolism*; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial; D004785:Environmental Pollutants; D010416:Pentachlorophenol