A quantitative thin layer chromatography method for determination of theophylline in plasma.
A simple assay method for theophylline in plasma using thin layer chromatography (TLC) was developed. The method involves extraction of the drug and internal standard (acetaminophen) by chloroform-isopropanol (75:25) followed by separation on TLC silica plates using a mixture of acetic acid, isopropanol, toluene (1: 12: 6), as the eluting solvent. Both peak height ratios and peak are ratios showed high correlation coefficient (r>0.98, p<0.001). However we used peak heights for the determinations. Within-day and between-day coefficients of variation were less than 4.4% and 7.8% respectively. The assay proved inexpensive, accurate and reproducible with a limit of detection of 100 ng/ml that makes it suitable for bioavailability studies.
Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
A
A
Mirfazaelian
M
M
Goudarzi
M
M
Tabatabaiefar
M
M
Mahmoudian
D001682:Biological Availability; D002138:Calibration; D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D013806:Theophylline / Q000097:blood*
D013806:Theophylline