Integrated microfluidic system enabling (bio)chemical reactions with on-line MALDI-TOF mass spectrometry.
A continuous flow micro total analysis system (micro-TAS) consisting of an on-chip microfluidic device connected to a matrix assisted laser desorption ionization [MALDI] time-of-flight [TOF] mass spectrometer (MS) as an analytical screening system is presented. Reaction microchannels and inlet/outlet reservoirs were fabricated by powderblasting on glass wafers that were then bonded to silicon substrates. The novel lab-on-a-chip was realized by integrating the microdevice with a MALDI-TOFMS standard sample plate used as carrier to get the microfluidic device in the MALDI instrument. A novel pressure-driven pumping mechanism using the vacuum of the instrument as a driving force induces flow in the reaction microchannel in a self-activating way. Organic syntheses as well as biochemical reactions are carried out entirely inside the MALDI-MS ionization vacuum chamber and analyzed on-line by MALDI-TOFMS in real time. The effectiveness of the micro-TAS system has been successfully demonstrated with several examples of (bio)chemical reactions.
Brivio
Monica
M
Laboratory of Supramolecular Chemistry and Technology, MESA+ Research Institute, University of Twente, Enschede, The Netherlands.
Fokkens
Roel H
RH
Verboom
Willem
W
Reinhoudt
David N
DN
Tas
Niels R
NR
Goedbloed
Martijn
M
van den Berg
Albert
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D009841:Oligonucleotides / Q000032:analysis; D009862:Online Systems; D010455:Peptides / Q000032:analysis; D017421:Sequence Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009841:Oligonucleotides; D010455:Peptides