Conditional control of selectin ligand expression and global fucosylation events in mice with a targeted mutation at the FX locus.
Glycoprotein fucosylation enables fringe-dependent modulation of signal transduction by Notch transmembrane receptors, contributes to selectin-dependent leukocyte trafficking, and is faulty in leukocyte adhesion deficiency (LAD) type II, also known as congenital disorder of glycosylation (CDG)-IIc, a rare human disorder characterized by psychomotor defects, developmental abnormalities, and leukocyte adhesion defects. We report here that mice with an induced null mutation in the FX locus, which encodes an enzyme in the de novo pathway for GDP-fucose synthesis, exhibit a virtually complete deficiency of cellular fucosylation, and variable frequency of intrauterine demise determined by parental FX genotype. Live-born FX(-/-) mice exhibit postnatal failure to thrive that is suppressed with a fucose-supplemented diet. FX(-/-) adults suffer from an extreme neutrophilia, myeloproliferation, and absence of leukocyte selectin ligand expression reminiscent of LAD-II/CDG-IIc. Contingent restoration of leukocyte and endothelial selectin ligand expression, general cellular fucosylation, and normal postnatal physiology is achieved by modulating dietary fucose to supply a salvage pathway for GDP-fucose synthesis. Conditional control of fucosylation in FX(-/-) mice identifies cellular fucosylation events as essential concomitants to fertility, early growth and development, and leukocyte adhesion.
Howard Hughes Medical Institute, The University of Michigan Medical School, 1150 W. Medical Center Drive, Ann Arbor, MI 48109, USA.
Peter L
PL
Smith
Jay T
JT
Myers
Clare E
CE
Rogers
Lan
L
Zhou
Bronia
B
Petryniak
Daniel J
DJ
Becker
Jonathon W
JW
Homeister
John B
JB
Lowe
D000818:Animals; D030801:Animals, Genetically Modified; D002238:Carbohydrate Epimerases / Q000235:genetics / Q000378:metabolism*; D019587:Dietary Supplements; D004622:Embryo, Mammalian / Q000002:abnormalities; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005328:Fetal Viability; D005643:Fucose / Q000378:metabolism*; D005838:Genotype; D016023:Integrins / Q000378:metabolism*; D007658:Ketone Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000235:genetics / Q000378:metabolism; D007964:Leukocytosis / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D011134:Polysaccharides / Q000378:metabolism; D019087:Selectins / Q000378:metabolism*
D029968:Escherichia coli Proteins; D016023:Integrins; D009097:Multienzyme Complexes; D011134:Polysaccharides; D019087:Selectins; C110682:wcaG protein, E coli; D005643:Fucose; D007658:Ketone Oxidoreductases; D002238:Carbohydrate Epimerases