Clinical isolates of Staphylococcus aureus with ribosomal mutations conferring resistance to macrolides.
Six strains of Staphylococcus aureus isolated from cystic fibrosis patients after treatment with azithromycin were cross-resistant to azithromycin and erythromycin. None of the isolates contained erm or msr(A) genes, but they all carried either A2058G/U or A2059G mutations within the rrl genes, with a majority of the rRNA copies bearing the mutation. One strain displayed an additional mutation in the rplV gene, encoding the L22 ribosomal protein.
Prunier
Anne-Laure
AL
Service de Microbiologie, CHU Côte de Nacre, Caen, France.
Malbruny
Brigitte
B
Tandé
Didier
D
Picard
Bertrand
B
Leclercq
Roland
R
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D003550:Cystic Fibrosis / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D004351:Drug Resistance; D004917:Erythromycin / Q000494:pharmacology; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D026901:Membrane Transport Proteins; D008780:Methyltransferases; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics*; D015345:Oligonucleotide Probes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012270:Ribosomes / Q000235:genetics*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; D015345:Oligonucleotide Probes; C066202:msrA protein, Staphylococcus epidermidis; D004917:Erythromycin; D017963:Azithromycin; D008780:Methyltransferases; C431709:ErmA protein, Bacteria