Genomic organization and fine-mapping of the human leucine zipper-bearing kinase (LZK) gene.
Leucine zipper-bearing kinase (LZK) is a new member of the mixed lineage protein kinase family. We previously cloned a cDNA encoding LZK from a human cerebellum cDNA library. The following studies indicated that LZK serves as a MAPKKK in the JNK/SAPK pathway in cells, and a scaffold protein, JIP-1, enhances LZK-induced JNK/SAPK pathway activation via physical association. Here we report characterization of the gene structure and fine chromosomal mapping of the human LZK gene. Polymerase chain-reaction (PCR) studies indicated that the human LZK coding sequence is composed of 13 exons, and that all the splice acceptor and donor sequences obey the GT-AG rule. Chromosomal localization studies involving FISH mapping demonstrated that the human LZK gene is located at 3q27.
Department of Biological Chemistry and CREST (Core Research for Educational Science and Technology) Project, Japan Science and Technology Corporation, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
Atsushi
A
Ikeda
Hiroyuki
H
Sakuma
Megumi
M
Masaki
Yasunori
Y
Kozutsumi
Shogo
S
Oka
Johji
J
Inazawa
Toshisuke
T
Kawasaki
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D016350:Leucine Zippers / Q000235:genetics; D020930:MAP Kinase Kinase Kinases / Q000235:genetics*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D018076:DNA, Complementary; D020930:MAP Kinase Kinase Kinases; C109162:MAP3K13 protein, human