The Epstein-Barr virus immediate-early protein BZLF1 induces both a G(2) and a mitotic block.
The Epstein-Barr virus immediate-early protein BZLF1 is a transcriptional activator that mediates the switch from latent to lytic infection. Here we demonstrate that BZLF1 induces both a G(2) block and a mitotic block in HeLa cells and inhibits chromosome condensation. While the G(2) block is associated with decreased cyclin B1 in host cells and can be rescued by overexpression of cyclin B1, the mechanism for the mitotic defect is as yet undetermined.
Mauser
Amy
A
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7295, USA.
Holley-Guthrie
Elizabeth
E
Simpson
Dennis
D
Kaufmann
William
W
Kenney
Shannon
S
D019926:Cyclin B / Q000502:physiology; D056744:Cyclin B1; D004268:DNA-Binding Proteins / Q000502:physiology*; D016195:G2 Phase; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D015534:Trans-Activators / Q000502:physiology*; D014764:Viral Proteins
C063676:BZLF1 protein, Herpesvirus 4, Human; C534699:CCNB1 protein, human; D019926:Cyclin B; D056744:Cyclin B1; D004268:DNA-Binding Proteins; D015534:Trans-Activators; D014764:Viral Proteins