Quantitation of platelet adhesion to Ti and DLC-coated Ti in vitro using (125)I-labeled platelets.
Measurement of platelet adhesion in vitro is a good indicator of its reactivity to implant devices in vivo. Platelets were labeled with I-125 without affecting its normal morphology and function and the labeled platelets were suspended in platelet poor plasma and exposed to Ti and diamond like carbon-coated (DLC) Ti discs, under dynamic conditions, using a parallel plate flow chamber. The test materials were washed, dried, exposed to a phosphor screen and scanned to get the images. The number of platelets that adhered to Ti was found to be higher than those that adhered to DLC coated Ti sample, irrespective of the shear stress which was varied between 2 and 16 dynes/cm(2).
Krishnan
L K
LK
BMT Wing, Si Chitra Tirunal Institute of Medical Sciences, Thiruvanandhapuram 690512, India.
Varghese
N
N
Muraleedharan
C V
CV
Bhuvaneshwar
G S
GS
Derangère
F
F
Sampeur
Y
Y
Suryanarayanan
R
R
D001792:Blood Platelets / Q000000981:diagnostic imaging* / Q000502:physiology; D002244:Carbon; D002448:Cell Adhesion; D002452:Cell Count; D020099:Coated Materials, Biocompatible; D018130:Diamond; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D008422:Materials Testing / Q000379:methods*; D010973:Platelet Adhesiveness; D011877:Radionuclide Imaging; D014025:Titanium
D020099:Coated Materials, Biocompatible; D007457:Iodine Radioisotopes; D002244:Carbon; D018130:Diamond; D014025:Titanium