Prevotella intermedia native plasmid can be mobilized by an Escherichia coli conjugal IncP plasmid.
We have determined the nucleotide sequence of a small Prevotella intermedia cryptic plasmid, pYHBi1, which consisted of sequences that were highly homologous to the amino acid sequence of the replication and mobilization proteins found in related organisms. We have also demonstrated that chimeric plasmids derived from this P. intermedia native plasmid can be mobilized between Escherichia coli strains by using a broad-host-range E. coli conjugative plasmid, IncP plasmid RP4. The results suggest that pYHBi1 possesses gene(s) responsible for conjugal transfer.
US Army Dental Research Detachment, Microbiology Branch, Walter Reed Army Institute of Research, 310B, B Street, Building 1-H, Great Lakes, IL 60088, USA. kai.leung@amedd.army.mil
K-P
KP
Leung
A S
AS
Lewis
S P
SP
Concannon
H
H
Yoshimoto
H
H
Fukushima
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003227:Conjugation, Genetic; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics*; D033903:Periplasmic Proteins; D010957:Plasmids / Q000235:genetics*; D018713:Prevotella intermedia / Q000235:genetics*; D015183:Restriction Mapping
D001426:Bacterial Proteins; D017931:DNA Primers; D033903:Periplasmic Proteins