Distinct response of human B cell subpopulations in recognition of an innate immune signal, CpG DNA.
Innate immunity has recently gained renewed interest in its ability to regulate adaptive immunity. Among the innate immune signals, CpG DNA has revealed its potential as a vaccine adjuvant. However, the cellular mechanism for the effect of CpG DNA on the humoral immune response is not well understood. Here, we investigated the effects of CpG DNA on human B cell differentiation using highly purified B cell subsets: naive, germinal center (GC), and memory B cells. In the in vitro culture system that mimics the primary or secondary immune response in vivo, CpG DNA markedly augmented the proliferation and generation of plasma cells from naive and memory B cells. CpG DNA dramatically increased plasma cell generation from GC B cells. However, CpG DNA did not have effect on memory B cell generation from GC B cells. These results suggest that CpG DNA potentiates the B cell adaptive immune response by enhancing terminal differentiation, but does not affect the generation of memory B cells.
Laboratory of Cellular Immunology, Ochsner Clinic Foundation, New Orleans, LA 70121, USA.
Jaeho
J
Jung
Ae-Kyung
AK
Yi
Xin
X
Zhang
Jongseon
J
Choe
Li
L
Li
Yong Sung
YS
Choi
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D018899:CpG Islands / Q000276:immunology*; D004247:DNA / Q000276:immunology / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D018858:Germinal Center / Q000166:cytology / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology; D007399:Interphase / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000276:immunology / Q000494:pharmacology*; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology*
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D004247:DNA; C427203:calf thymus DNA