A desaturase-like protein from white spruce is a Delta(9) desaturase.
Gymnospermae seed lipids are characterized by a high degree of desaturation, most having a Delta(9) double bond. By degenerate polymerase chain reaction (PCR) we have isolated a white spruce (Picea glauca) cDNA clone that encodes an amino acid sequence sharing a high degree of homology with other putative plant acyl-coenzyme A (CoA) Des9 desaturases. Both in vivo and in vitro expression studies in a Delta(9) desaturase-deficient yeast strain demonstrated the desaturation functionality of the white spruce clone, and gas chromatography-mass spectrometry (GC-MS) analyses confirmed the regioselectivity of the encoded enzyme. This is the first report of the functional characterization of a plant membrane-bound acyl-CoA-like protein Delta(9) desaturase by heterologous expression in yeast.
National Research Council of Canada, Plant Biotechnology Institute, Seed Oil Biotechnology Group, 110 Gymnasium Place, Saskatoon, SK, Canada S7N 0W9.
Elizabeth-France
EF
Marillia
E Michael
EM
Giblin
Patrick S
PS
Covello
David C
DC
Taylor
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D032462:Cycadopsida / Q000201:enzymology*; D017931:DNA Primers; D007700:Kinetics; D010446:Peptide Fragments / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014197:Trees / Q000201:enzymology
D017931:DNA Primers; D010446:Peptide Fragments; D011994:Recombinant Proteins; D013230:Stearoyl-CoA Desaturase