Technical improvement to prevent DNA degradation of enteric pathogens in pulsed-field gel electrophoresis.
This study used a modified pulsed-field gel electrophoresis (PFGE) method with HEPES as a running buffer to prevent electrophoresis-related DNA degradation of nine Salmonella enterica subsp. enterica serovar Ohio, seven Salmonella serovar Newport, and two enterohemorrhagic Escherichia coli (non-O157) strains. All strains yielded identifiable bands with this method in contrast to a commonly applied PFGE method using Tris buffer.
Laboratory of Enteric Pathogens, National Public Health Institute, Helsinki, Finland.
Joanna M K
JM
Koort
Susanna
S
Lukinmaa
Marjatta
M
Rantala
Erja
E
Unkila
Anja
A
Siitonen
D015373:Bacterial Typing Techniques / Q000379:methods*; D002021:Buffers; D004269:DNA, Bacterial / Q000737:chemistry*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D004926:Escherichia coli / Q000145:classification / Q000235:genetics*; D006531:HEPES; D006801:Humans; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics*
D002021:Buffers; D004269:DNA, Bacterial; D006531:HEPES