Crystallization and preliminary crystallographic studies of pyridoxal kinase from sheep brain.
Pyridoxal kinase (ATP:pyridoxal 5'-phosphotransferase; EC 2.7.1.35) is a key enzyme in the transformation of vitamin B(6) to pyridoxal-5'-phosphate. Pyridoxal-5'-phosphate is the crucial cofactor required by numerous enzymes involved in the metabolism of amino acids and the synthesis of many neurotransmitters. Pyridoxal kinase from sheep brain was crystallized in an orthorhombic form using the hanging-drop vapour-diffusion method with sodium citrate as the precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 59.8, b = 94.4, c = 128.2 A, and diffract to a resolution of 2.1 A. Crystals were transferred into a soaking liquid without citrate and two heavy-atom derivatives were prepared.
Li
Ming-Hui
MH
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, People's Republic of China.
Kwok
Francis
F
An
Xiao-Min
XM
Chang
Wen-Rui
WR
Lau
Chi-Kong
CK
Zhang
Ji-Ping
JP
Liu
Sheng-Quan
SQ
Leung
Yun-Chung
YC
Jiang
Tao
T
Liang
Dong-Cai
DC
D000818:Animals; D001921:Brain / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D012756:Sheep