[Experimental study on microvascular architecture of human and rabbit flaps with two methods].
OBJECTIVE
To study effective method of showing the microvascular architecture of human and rabbit skin flaps.


METHODS
According to endogenous alkaline phosphatase activity, Gomori-Takamatsu method and NBT/BCIP method were used to observe the microvascular architecture.


RESULTS
Both methods had capability to demonstrate the microvascular architecture of the human skin flap, whereas the examining results of rabbits were negative.


CONCLUSIONS
Great difference of endogeneous alkaline phosphatase activity exists in different animals. Gomori-Takamatsu method and NBT/BCIP method are useful in study of microvascular architecture of human skin flaps.
China-Japan Friendship Hospital, Beijing 100029, China.
Hongwei
H
Zhang
Lin
L
Pan
Hui
H
Wang
Xiaochen
X
Tian
Zhe
Z
Cai
Yeguang
Y
Song
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D006801:Humans; D008297:Male; D008833:Microcirculation / Q000033:anatomy & histology; D011817:Rabbits; D013524:Surgical Flaps / Q000098:blood supply*
D000469:Alkaline Phosphatase