The tRNA-Tyr gene family of Saccharomyces cerevisiae: agents of phenotypic variation and position effects on mutation frequency.
Extensive phenotypic diversity or variation exists in clonal populations of microorganisms and is thought to play a role in adaptation to novel environments. This phenotypic variation or instability, which occurs by multiple mechanisms, may be a form of cellular differentiation and a stochastic means for modulating gene expression. This work dissects a case of phenotypic variation in a clinically derived Saccharomyces cerevisiae strain involving a cox15 ochre mutation, which acts as a reporter. The ochre mutation reverts to sense at a low frequency while tRNA-Tyr ochre suppressors (SUP-o) arise at a very high frequency to produce this phenotypic variation. The SUP-o mutations are highly pleiotropic. In addition, although all SUP-o mutations within the eight-member tRNA-Tyr gene family suppress the ochre mutation reporter, there are considerable phenotypic differences among the different SUP-o mutants. Finally, and of particular interest, there is a strong position effect on mutation frequency within the eight-member tRNA-Tyr gene family, with one locus, SUP6, mutating at a much higher than average frequency and two other loci, SUP2 and SUP8, mutating at much lower than average frequencies. Mechanisms for the position effect on mutation frequency are evaluated.
Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Sayoko
S
Ito-Harashima
Phillip E
PE
Hartzog
Himanshu
H
Sinha
John H
JH
McCusker
D016153:Genes, Suppressor; D014644:Genetic Variation; D008565:Membrane Proteins / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D012365:RNA, Transfer, Tyr / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
C107633:COX15 protein, S cerevisiae; D008565:Membrane Proteins; D012365:RNA, Transfer, Tyr; D029701:Saccharomyces cerevisiae Proteins