A Dirofilaria immitis polyprotein up-regulates nitric oxide production.
We investigated the effect of recombinant Dirofilaria immitis polyprotein (rDiAg) on nitric oxide (NO) production by peritoneal macrophages. rDiAg induced NO production by macrophages from wild-type and lipopolysaccharide-hyporesponsive C3H/HeJ, but not CD40(-/-), mice. These results suggest that CD40 is involved in rDiAg-driven NO production by murine macrophages.
Section of Environmental Parasitology, Department of International Health Development, Division of Public Health, Graduate School, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8519, Japan.
Hiroyuki
H
Tezuka
Shinjiro
S
Imai
Setsuko
S
Tsukidate
Koichiro
K
Fujita
D000818:Animals; D000947:Antigens, Helminth / Q000502:physiology; D001483:Base Sequence; D019013:CD40 Antigens / Q000235:genetics / Q000378:metabolism; D004183:Dirofilaria immitis / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D015801:Helminth Proteins / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D006790:Host-Parasite Interactions; D066298:In Vitro Techniques; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D015854:Up-Regulation
D000947:Antigens, Helminth; D019013:CD40 Antigens; D015801:Helminth Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse