Separation and detection of angiotensin peptides by Cu(II) complexation and capillary electrophoresis with UV and electrochemical detection.
The use of capillary electrophoresis (CE) with on-capillary Cu(II) complexation for the determination of angiotensin and its metabolites is described. The resulting copper-peptide complexes can be detected using either UV or electrochemical (EC) detection. Optimal reaction and separation conditions for the angiotensin peptides were first determined using CE with UV detection. With UV detection, the limit of detection (signal-to noise ratio S/N = 3) for native angiotensin II was 18 microM, while the limit of detection (LOD) obtained for the copper-angiotensin II complex is 2 microM. CE with EC detection was then evaluated, yielding significantly lower LODs--2 microM for native angiotensin II and 200 nM for the copper-angiotensin II complex. The addition of copper to the run buffer improved the separation and sensitivity for both CE-UV and CE-EC detection. The method was demonstrated by monitoring the conversion of angiotensin I to angiotensin II in plasma via angiotensin-converting enzyme (ACE) and subsequent inhibition of ACE by captopril.
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS, USA.
Nathan A
NA
Lacher
Kenneth E
KE
Garrison
Susan M
SM
Lunte
D000803:Angiotensin I / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism; D000804:Angiotensin II / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism; D000809:Angiotensins / Q000097:blood / Q000302:isolation & purification* / Q000378:metabolism; D001738:Biuret Reaction; D003300:Copper / Q000737:chemistry*; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
D000809:Angiotensins; D000804:Angiotensin II; D003300:Copper; D000803:Angiotensin I