Cloning and characterization of rhesus monkey MCH-R1 and MCH-R2.
Rhesus monkey MCH-R1 and MCH-R2 receptors were cloned. Amino acid homology is 98.8% between monkey and human MCH-R1, while monkey and human MCH-R2 are 98% homologous. Binding and intracellular signaling characteristics of the monkey receptors were compared with the human homologues. The results demonstrate that MCH binds to the monkey MCH-R1 receptor with a K(d) of 6.5 nM and monkey MCH-R2 with a K(d) of 2.2 nM similar to K(d) values for human MCH-R1 and MCH-R2. Additionally, monkey MCH-R1 couples through G(i)/G(o) and G(q)-type G proteins similar to human MCH-R1 whereas monkey and human MCH-R2 utilize the G(q) signaling pathway.
Schering-Plough Research Institute, CNS/CV Biological Research, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA.
Steven
S
Fried
Kim
K
O'Neill
Brian E
BE
Hawes
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D007028:Hypothalamic Hormones / Q000378:metabolism; D008253:Macaca mulatta; D008543:Melanins / Q000378:metabolism; D008969:Molecular Sequence Data; D010907:Pituitary Hormones / Q000378:metabolism; D016133:Polymerase Chain Reaction; D043562:Receptors, G-Protein-Coupled; D011979:Receptors, Pituitary Hormone / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
D007028:Hypothalamic Hormones; C431280:MCHR2 protein, human; D008543:Melanins; D010907:Pituitary Hormones; D043562:Receptors, G-Protein-Coupled; D011979:Receptors, Pituitary Hormone; C092274:melanin-concentrating hormone receptor; C019463:melanin-concentrating hormone; D000242:Cyclic AMP; D002118:Calcium