Tag-reporter and resin capture--release strategy in oligosaccharide synthesis.
Low molecular weight poly(ethylene glycol) (LWPEG) has been found to be useful as a "tag" in oligosaccharide synthesis due to its high polarity. Real-time reaction monitoring was achieved by use of MALDI-TOF MS in the glycosylation reactions and color tests in the deprotection of the chloroacetyl group. Further, a cysteine-supported insoluble resin enables the purification of the chloroacetyl-bound compounds on soluble PEG.
RIKEN, The Institute of Physical and Chemical Research, Wako-shi, Saitama, Japan. yukito@postman.riken.go.jp
Yukishige
Y
Ito
Shino
S
Manabe
D020650:Combinatorial Chemistry Techniques / Q000295:instrumentation / Q000379:methods*; D009844:Oligosaccharides / Q000032:analysis / Q000138:chemical synthesis* / Q000302:isolation & purification; D009862:Online Systems; D011092:Polyethylene Glycols; D012117:Resins, Synthetic / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009844:Oligosaccharides; D012117:Resins, Synthetic; D011092:Polyethylene Glycols