[Screening and identification of tumor related genes in early stages of transformed NIH3T3 cells associated with overexpressed PTPalpha].
An inducible-PTPalpha expression system was used to screen differential expression genes in NIH3T3 cells overexpressing PTPalpha, which could be used as a model for studying mechanism of tumorigenesis at early stage. After PTPalpha induction for 24 h, 65 differential bands were obtained, among which, there were 29 known genes, 12 known EST, 6 novel EST by bioinformatic analysis. Functions of the known genes were related to signal transduction, structural/adhesion, energy metabolism, transcription/translation, ribosome and apoptosis. The differential expression of the genes coding for G protein, TCR, Trx-related protein, caveolin-1 was verified by RT-PCR and Northern blot. The results showed that the expression of numerous genes was changed after the induction of PTPalpha for 24 h, these genes are related to many cellular physiological functions, and some of them play important roles in the early stage of tumorigenesis. The results provide basis for further exploring the molecular mechanism of tumorigenesis and these genes may well be candidate targets for gene therapy.
Yang
Jin-Song
JS
Department of Biochemistry, Laboratory of Molecular Biology and Research Center for Human Gene Therapy, Shanghai Second Medical University, Shanghai 200025, China. sschen@shsmu.edu.cn
Zheng
Xin-Min
XM
Chen
Shi-Shu
SS
D016475:3T3 Cells; D000818:Animals; D002461:Cell Line, Transformed; D004587:Electrophoresis, Agar Gel / Q000379:methods; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D009363:Neoplasm Proteins; D017027:Protein Tyrosine Phosphatases