Calcium wave pacemakers in eggs.
During the past 25 years, the characterization of sperm-triggered calcium signals in eggs has progressed from the discovery of a single calcium increase at fertilization in the medaka fish to the observation of repetitive calcium waves initiated by multiple meiotic calcium wave pacemakers in the ascidian. In eggs of all animal species, sperm-triggered inositol (1,4,5)-trisphosphate [Ins(1,4,5)P(3)] production regulates the vast array of calcium wave patterns observed in the different species. The spatial organization of calcium waves is driven either by the intracellular distribution of the calcium release machinery or by the localized and dynamic production of calcium-releasing second messengers. In the highly polarized egg cell, cortical endoplasmic reticulum (ER)-rich clusters act as pacemaker sites dedicated to the initiation of global calcium waves. The extensive ER network made of interconnected ER-rich domains supports calcium wave propagation throughout the egg. Fertilization triggers two types of calcium wave pacemakers depending on the species: in mice, the pacemaker site in the vegetal cortex of the egg is probably a site that has enhanced sensitivity to Ins(1,4,5)P(3); in ascidians, the calcium wave pacemaker may rely on a local source of Ins(1,4,5)P(3) production apposed to a cluster of ER in the vegetal cortex.
Dumollard
Rémi
R
Bio Mar Cell, Unité de Biologie du Développement UMR 7009 CNRS/Paris VI, Observatoire, Station Zoologique, Villefranche sur Mer, 06230 France. r.dumollard@ucl.ac.uk
Carroll
John
J
Dupont
Geneviève
G
Sardet
Christian
C
D000818:Animals; D001683:Biological Clocks / Q000502:physiology*; D020013:Calcium Signaling / Q000502:physiology*; D016764:Cell Polarity / Q000502:physiology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005306:Fertilization / Q000502:physiology*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D010063:Ovum / Q000166:cytology / Q000378:metabolism*; D015290:Second Messenger Systems / Q000502:physiology
D015544:Inositol 1,4,5-Trisphosphate