[The characters of proliferating activity and biosynthetic function of fibroblasts from abnormal scars after complete contact in vitro].
OBJECTIVE
This study was to determine the proliferating and biosynthetic characters of fibroblasts from abnormal scars and normal skins after complete contact in vitro.


METHODS
6 samples of keloid, hypertrophic scar and normal skin were collected respectively. Proliferation, inhibition and biosyntheses of different fibroblasts were investigated by detecting proliferating cell nuclear antigen(PCNA), P16, type I and III collagen proteins, and Pro alpha 1 (I) and Pro alpha 1 (III) procollagen gene expressions under the condition of fibroblast complete contact by means of cell culture, immunohistochemistry and molecular biological techniques, etc.


RESULTS
Overlap and high levels of proliferating activity and biosyntheses of complete contact fibroblasts from keloids indicated that these fibroblasts lost the behavior of contact inhibition and density inhibition. However, proliferating activity and biosynthetic function of complete contact fibroblasts from normal skins reduced dramatically. Biosynthetic function of complete contact fibroblasts from hypertrophic scars was still very strong, but proliferating activity was lower than keloid and higher than normal skin.


CONCLUSIONS
Proliferating and biosynthetic characters of complete contact fibroblasts from abnormal scars and normal skins were different, which may play an important role in the mechanisms of abnormal scar formation.
Research Center of Plastic Surgery, Third Clinical School of Medicine, Beijing University, Beijing 100083, China.
Shaojun
S
Xu
Weihan
W
Bao
Dongming
D
Chen
Qi
Q
Wang
D000328:Adult; D002455:Cell Division; D017439:Cicatrix, Hypertrophic / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000096:biosynthesis*; D005260:Female; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis
D018809:Proliferating Cell Nuclear Antigen; D003094:Collagen