The presence of chlorophyll b in Synechocystis sp. PCC 6803 disturbs tetrapyrrole biosynthesis and enhances chlorophyll degradation.
Both chlorophyll (Chl) a and b accumulate in the light in a Synechocystis sp. PCC 6803 strain that expresses higher plant genes coding for a light-harvesting complex II protein and Chl a oxygenase. This cyanobacterial strain also lacks photosystem (PS) I and cannot synthesize Chl in darkness because of the lack of chlL. When this PS I-less/chlL(-)/lhcb(+)/cao(+) strain was grown in darkness, small amounts of two unusual tetrapyrroles, protochlorophyllide (PChlide) b and pheophorbide (pheide) b, were identified. Accumulation of PChlide b trailed that of PChlide a by several days, suggesting that PChlide a is an inefficient substrate of Chl a oxygenase. The presence of pheide b in this organism suggests a breakdown of Chl b via a pathway that does not involve conversion to a-type pigments. When the PS I-less/chlL(-) control strain was grown in darkness, Chl degradation was much slower than in the PS I-less/chlL(-)/lhcb(+)/cao(+) strain, suggesting that the presence of Chl b leads to more rapid turnover of Chl-binding proteins and/or a more active Chl degradation pathway. Levels and biosynthesis kinetics of Chl and of its biosynthetic intermediates are very different in the PS I-less/chlL(-)/lhcb(+)/cao(+) strain versus in the control. Moreover, when grown in darkness for 14 days, upon the addition of delta-aminolevulinic acid, the level of magnesium-protoporphyrin IX increased 60-fold in the PS I-less/chlL(-)/lhcb(+)/cao(+) strain (only approximately 2-fold in the PS I-less/chlL(-) control strain), whereas the PChlide and protoheme levels remained fairly constant. We propose that a b-type PChlide, Chl, or pheide in the PS I-less/chlL(-)/lhcb(+)/cao(+) strain may bind to tetrapyrrole biosynthesis regulatory protein(s) (for example, the small Cab-like proteins) and thus affect the regulation of this pathway.
Xu
Hong
H
Department of Plant Biology and Center for the Study of Early Events in Photosynthesis, Arizona State University, Tempe, Arizona 85287-1601, USA.
Vavilin
Dmitrii
D
Vermaas
Wim
W
D002734:Chlorophyll / Q000096:biosynthesis / Q000378:metabolism*; D000458:Cyanobacteria / Q000254:growth & development / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism; D011758:Pyrroles / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D045725:Tetrapyrroles
D045322:Photosynthetic Reaction Center Complex Proteins; D011758:Pyrroles; D045725:Tetrapyrroles; D002734:Chlorophyll; C037184:chlorophyll b