Comparison of different setups for one- and two-dimensional capillary high-performance liquid chromatography and pressurized capillary electrochromatography coupled on-line with mass spectrometry.
A comparison of different separation methods (high-performance liquid chromatography (HPLC), capillary HPLC (CHPLC) and pressurized capillary electrochromatography (pCEC)) coupled on-line with mass spectrometry (MS) is undertaken using the separation of a crude extract of ergot fungus (secalis cornuti) as an example. New and simple setups for a two-dimensional CHPLC coupled on-line with electrospray ionization (ESI)-MS (2D-CHPLC-MS) as well as for capillary size-exclusion chromatography performed under pCEC conditions and coupled on-line with ESI-MS (CSEC-pCEC-MS) are shown. In addition, an improved method for column packing is presented.
Institut für Organische Chemie, Universität Tübingen, Tübingen, Germany. mark.stahl@uni-tuebingen.de
Mark
M
Stahl
Andreas
A
Jakob
Alexander
A
von Brocke
Graeme
G
Nicholson
Ernst
E
Bayer
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000592:standards; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation* / Q000592:standards; D002967:Claviceps / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000592:standards; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000592:standards; D004867:Equipment Design; D004879:Ergotamines / Q000032:analysis / Q000302:isolation & purification; D009862:Online Systems; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000592:standards
D004879:Ergotamines; D010446:Peptide Fragments; D011506:Proteins