Aquatic insects as a vector for Mycobacterium ulcerans.
Mycobacterium ulcerans is an emerging environmental pathogen which causes chronic skin ulcers (i.e., Buruli ulcer) in otherwise healthy humans living in tropical countries, particularly those in Africa. In spite of epidemiological and PCR data linking M. ulcerans to water, the mode of transmission of this organism remains elusive. To determine the role of aquatic insects in the transmission of M. ulcerans, we have set up an experimental model with aquariums that mimic aquatic microenvironments. We report that M. ulcerans may be transmitted to laboratory mice by the bite of aquatic bugs (Naucoridae) that are infected with this organism. In addition, M. ulcerans appears to be localized exclusively within salivary glands of these insects, where it can both survive and multiply without causing any observable damage in the insect tissues. Subsequently, we isolated M. ulcerans from wild aquatic insects collected from a zone in the Daloa region of Ivory Coast where Buruli ulcer is endemic. Taken together, these results point to aquatic insects as a possible vector of M. ulcerans.
Marsollier
Laurent
L
Laboratoire de Bactériologie-Virologie-Hygiène, CHU, 49033 Angers, France.
Robert
Raymond
R
Aubry
Jacques
J
Saint André
Jean-Paul
JP
Kouakou
Henri
H
Legras
Pierre
P
Manceau
Anne-Lise
AL
Mahaza
Chetaou
C
Carbonnelle
Bernard
B
D000818:Animals; D004195:Disease Models, Animal; D004199:Disease Vectors; D005260:Female; D007313:Insecta / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009165:Mycobacterium Infections, Nontuberculous / Q000635:transmission*; D019911:Mycobacterium ulcerans / Q000235:genetics; D016133:Polymerase Chain Reaction; D012469:Salivary Glands / Q000382:microbiology