Pulmonary responses to tracheal or esophageal acidification in guinea pigs with airway inflammation.
The association between asthma and gastroesophageal reflux has been attributed to microaspiration of gastric contents and/or vagally mediated reflex bronchoconstriction. In previous experimental studies concerning the pulmonary effects of tracheal or esophageal acid infusion, only animals without airway inflammation have been studied. We assessed the effects of esophageal and tracheal administration of hydrochloric acid (HCl) on normal guinea pigs (GP) and GP with airway inflammation induced by repeated ovalbumin exposures. These GP were anesthetized (pentobarbital sodium) and received 1) 20 microl of either 0.2 N HCl or saline into the trachea, or 2) 1 ml of either 1 N HCl or saline into the esophagus. Intratracheal HCl resulted in a significant increase in both respiratory system elastance and resistance (P < 0.001). There were no significant changes in respiratory mechanics when HCl was infused into the esophagus. In conclusion, we observed that infusion of large volumes of HCl into the esophagus did not change pulmonary mechanics significantly, even in guinea pigs with chronic allergen-induced airway inflammation. In contrast, intratracheal administration of small amounts of acid had substantial effects in normal GP and GP with airway inflammation.
Lopes
Fernanda D T Q S
FD
Department of Medicine, School of Medicine, University of Sao Paulo, 01246-903 Sao Paulo, Brazil.
Alvarenga
Glauco S
GS
Quiles
Raquel
R
Dorna
Mayra B
MB
Vieira
Joaquim E
JE
Dolhnikoff
Marisa
M
Martins
Milton A
MA
D000403:Airway Resistance / Q000187:drug effects; D000818:Animals; D004548:Elasticity; D004947:Esophagus / Q000187:drug effects / Q000503:physiopathology*; D006168:Guinea Pigs; D006851:Hydrochloric Acid / Q000008:administration & dosage* / Q000494:pharmacology; D008168:Lung / Q000503:physiopathology*; D008297:Male; D011014:Pneumonia / Q000503:physiopathology*; D012016:Reference Values; D015656:Respiratory Mechanics / Q000187:drug effects; D014132:Trachea / Q000187:drug effects / Q000503:physiopathology*
D006851:Hydrochloric Acid