Transcriptional programs activated by exposure of human prostate cancer cells to androgen.
BACKGROUND
Androgens are required for both normal prostate development and prostate carcinogenesis. We used DNA microarrays, representing approximately 18,000 genes, to examine the temporal program of gene expression following treatment of the human prostate cancer cell line LNCaP with a synthetic androgen.


RESULTS
We observed statistically significant changes in levels of transcripts of more than 500 genes. Many of these genes were previously reported androgen targets, but most were not previously known to be regulated by androgens. The androgen-induced expression programs in three additional androgen-responsive human prostate cancer cell lines, and in four androgen-independent subclones derived from LNCaP, shared many features with those observed in LNCaP, but some differences were observed. A remarkable fraction of the genes induced by androgen appeared to be related to production of seminal fluid and these genes included many with roles in protein folding, trafficking, and secretion.


CONCLUSIONS
Prostate cancer cell lines retain features of androgen responsiveness that reflect normal prostatic physiology. These results provide a broad view of the effect of androgen signaling on the transcriptional program in these cancer cells, and a foundation for further studies of androgen action.
Department of Urology, Room S 287, Stanford University School of Medicine, Stanford, CA 94305, USA. jdbrooks@stanford.edu
Samuel E
SE
DePrimo
Maximilian
M
Diehn
Joel B
JB
Nelson
Robert E
RE
Reiter
John
J
Matese
Mike
M
Fero
Robert
R
Tibshirani
Patrick O
PO
Brown
James D
JD
Brooks
D000728:Androgens / Q000494:pharmacology*; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008297:Male; D015741:Metribolone / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D045165:Testosterone Congeners / Q000494:pharmacology; D015533:Transcriptional Activation / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D000728:Androgens; D045165:Testosterone Congeners; D015741:Metribolone