Induction of fetal diuresis with intraamniotic furosemide increases the clearance of intraamniotic substances: An alternative therapy aimed at reducing intraamniotic meconium concentration.
OBJECTIVE
Contact with amniotic fluid (AF) causes intestinal damage in gastroschisis. Intraamniotic meconium has been shown to be responsible for intestinal damage, and occurrence of this damage has been shown to depend on the concentration of intraamniotic meconium. When intraamniotic meconium concentration is lowered below threshold level by exchanging AF with saline in gastroschisis, intestinal damage can be prevented. Theoretically, induction of fetal diuresis with intraamniotic furosemide may increase AF volume and fetal swallowing rate, thus, increase absorption of AF by intestines; therefore, the clearance of meconium from the AF may increase. An experimental study was planned to investigate the effects of intraamniotic diuretic injection on the clearance of intraamniotic substances.


METHODS
Pregnant rabbits on the 23rd to 25th gestational day were divided into 2 groups as furosemide and control. Technetium tc99m labeled "tin colloid" was injected into the amniotic cavity, and AF sample was taken 10 minutes later. Furosemide was injected into the amniotic cavity afterwards. Two and 6 hours later, AF samples were obtained. Intestines were harvested at the end of the study. Control group received intraamniotic saline instead of furosemide. Radioactivities of the AF samples and intestines were determined by gamma counter. Clearance of the radioisotope from AF and intestinal accumulation were calculated.


RESULTS
The clearance of the radioisotope from AF was increased significantly in the furosemide group (n = 10) compared with the control group (n = 8; P <.01). Gastrointestinal accumulation of the radioisotope in the furosemide group was 4-fold higher than that the control group (P <.01).


CONCLUSIONS
Induction of fetal diuresis with intraamniotic furosemide accelerates the clearance of intraamniotic substances. This is probably caused by increased urinary output rate, which increases AF volume and consequently results in increased fetal swallowing of AF. In the diseases like gastroschisis and myelomeningocele, in which the contact with AF causes tissue damage, the elimination of meconium from AF in a somewhat natural manner like this method, should be studied further because it may be an alternative minimal invasive in utero treatment modality.
Departments of Pediatric Surgery and Nuclear Medicine, Dokuz Eylül University, Medical School, Izmir, Turkey.
Gülce
G
Hakgüder
Oğuz
O
Ateş
Mustafa
M
Olguner
Arman
A
Api
Ozhan
O
Ozdoğan
Berna
B
Değirmenci
Feza M
FM
Akgür
D000653:Amniotic Fluid / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D004231:Diuresis / Q000187:drug effects; D004232:Diuretics / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005315:Fetal Diseases / Q000188:drug therapy / Q000378:metabolism*; D005665:Furosemide / Q000008:administration & dosage / Q000494:pharmacology*; D020139:Gastroschisis / Q000188:drug therapy / Q000196:embryology* / Q000378:metabolism; D008470:Meconium / Q000378:metabolism*; D011247:Pregnancy; D011817:Rabbits
D004232:Diuretics; D005665:Furosemide