[Cloning and expression of the cecropin B-thanatin hybrid antimicrobial peptide in Escherichia coli].
A 44-residue hybrid peptide (CB (1-24)-Arg-Ser-Tyr-Tan (4-21)) incorporating 1-24 residues of cecropin B (CB) and 4-21 residues of thanatin (Tan) was designed and constructed. The CB-Tan gene was cloned into expression plasmid pGEX-3X and expressed in E. coli BL21. The fusion protein was purified by affinity chromatography. After digested with enterokinase the gene product released with antibacterial activity and gave one band in Tricine-SDS-PAGE.
College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. cssswjsz@zaas.org
Hong-Biao
HB
Weng
Bao-Long
BL
Niu
Zhi-Qi
ZQ
Meng
Meng-Kui
MK
Xu
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D019476:Insect Proteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D019476:Insect Proteins; D010456:Peptides, Cyclic; D011993:Recombinant Fusion Proteins; C098076:thanatin; C031162:cecropin B protein, Insecta