Isolation, identification and characterization of chicken anaemia virus in Malaysia.
A study was conducted to isolate and identify chicken anaemia virus (CAV) from field samples of clinically infected broiler chickens in Malaysia. A total of 125 samples were collected from chickens aged 2-6 weeks with clinically depressed and retarded growth, of which five samples were found positive to CAV directly by polymerase chain reaction (PCR). Later, five isolates of CAV from the respective five PCR positive samples were isolated in MDCC-MSB1 cells at passage 4 based on cytopathic effects, PCR and indirect immunofluorescent antibody test. The isolates were identified as BL-1, BL-2, BL-3, BL-4 and BL-5. These CAV isolates were found to resist treatment with chloroform and heat at 37 degrees C for 2 h, 56 degrees C for 30 min and 70 degrees C for 5 min. One of the isolates, BL-5 produced significant reduction (p < 0.001) of hematocrit values (9-19%), pale bone marrow, thymus atrophy and haemorrhages in skin/muscle when inoculated into 1-day old SPF chickens. Restriction enzyme digestion of 926 bp genomic fragments of all the isolates including Cux-1 isolate with HindIII exhibited a similar pattern of bands in 2% agarose gel. The present findings confirmed the presence of CAV in Malaysia.
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
S M Z H
SM
Chowdhury
A R
AR
Omar
I
I
Aini
M
M
Hair-Bejo
A A
AA
Jamaluddin
Y
Y
Kono
A
A
Darus
H M
HM
Yatim
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D001853:Bone Marrow / Q000821:virology; D002460:Cell Line; D017727:Chicken anemia virus / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D002645:Chickens / Q000821:virology*; D002725:Chloroform / Q000494:pharmacology; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D019084:Fluorescent Antibody Technique, Indirect; D006358:Hot Temperature; D008296:Malaysia; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D012867:Skin / Q000821:virology; D013696:Temperature; D013950:Thymus Gland / Q000821:virology; D013997:Time Factors
D000956:Antigens, Viral; D002725:Chloroform; D004262:DNA Restriction Enzymes