A new type of capillary column for open-tubular electrochromatography.
A new type of open-tubular C(18) ester-bonded electrochromatographic column was prepared with sol-gel technology, followed by an on-column octadecyl silylation reaction. Glycidoxypropyltrimethoxysilane, a widely used and important silane agent, was used as the sol-gel precursor to form a thin coating layer on the wall of the fused-silica capillary. The C(18) groups were introduced into the coating layer by on-column esterification reaction with stearic acid. The electrochromatography behavior of the column was evaluated in terms of the separation of peptides. A high efficiency of 4.8x10(5) plates/m was achieved for a basic pentapeptide using the C(18 )ester-bonded column. In comparison with bare capillaries and glycidoxypropyltrimethoxysilane sol-gel-coated capillaries, the C(18) ester-bonded column showed the best separation of a mixture of seven pentapeptides under identical conditions of buffer, pH, and applied voltage.
Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, PR China.
Yingxin
Y
Zhao
Rui
R
Zhao
Dihua
D
Shangguan
Guoquan
G
Liu
D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D007202:Indicators and Reagents; D015203:Reproducibility of Results; D012821:Silanes / Q000138:chemical synthesis / Q000737:chemistry
D007202:Indicators and Reagents; D012821:Silanes