Single strand DNA breaks in T- and B-lymphocytes and granulocytes in workers exposed to benzene.
Comet assays were carried out to evaluate DNA damage in T- and B-lymphocytes and granulocytes from 41 workers exposed to benzene in a printing company and 41 unexposed donors. In T-lymphocytes, DNA damage was slightly higher in exposed workers than in controls. The tail moments in the two groups were 1.75+/-0.29 and 1.47+/-0.41, respectively (P<0.0006). DNA damage of B-lymphocytes in the two groups showed the most significant difference among the three cell types. The tail moments were 3.86+/-0.71 and 1.51+/-0.39, respectively (P<0.0001). In granulocytes, DNA damage was also different, the tail moments being 3.61+/-0.75 and 2.60+/-0.59, respectively (P<0.0001). The comparison of DNA damage in both groups shows that B-lymphocytes could be a useful target in biomonitoring of human exposure to low levels of benzene.
Sul
Donggeun
D
Department of Preventive Medicine, School of Medicine and Institute for Environmental Health, Medical Science Research Center, Korea University, Seoul, South Korea.
Lee
Doyoung
D
Im
Hosub
H
Oh
Eunha
E
Kim
Jooja
J
Lee
Eunil
E
D000328:Adult; D001402:B-Lymphocytes / Q000187:drug effects; D001554:Benzene / Q000009:adverse effects*; D020552:Comet Assay; D003404:Creatinine / Q000652:urine; D004249:DNA Damage / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000097:blood / Q000187:drug effects*; D006098:Granulocytes / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects* / Q000032:analysis; D011327:Printing; D013011:Sorbic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D013601:T-Lymphocytes / Q000187:drug effects
D004277:DNA, Single-Stranded; C005939:muconic acid; D003404:Creatinine; D001554:Benzene; D013011:Sorbic Acid