Evaluation of genotype and LiPA MYCOBACTERIA assays for identification of Finnish mycobacterial isolates.
Two DNA strip assays, INNO-LiPA MYCOBACTERIA and GenoType Mykobakterien, were evaluated for identification of 81 Finnish mycobacterial isolates. The LiPA assay correctly identified 89.4% of the 66 isolates studied, and the GenoType assay identified 95.1% of 81 isolates. The GenoType assay had a wider selection of species and less stringent temperature requirements.
National Public Health Institute. Turku Graduate School of Biomedical Sciences Department of Medical Microbiology, University of Turku, Turku, Finland.
Johanna
J
Mäkinen
Aleksi
A
Sarkola
Merja
M
Marjamäki
Matti K
MK
Viljanen
Hanna
H
Soini
D015373:Bacterial Typing Techniques; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D005387:Finland; D005838:Genotype; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics; D009164:Mycobacterium Infections / Q000382:microbiology*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips
D015342:DNA Probes; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips