[Effects of microfilaments on the expression of collagenases and tissue inhibitor of metalloproteinases-1 in fibroblasts].
OBJECTIVE
To investigate the roles of microfilaments in the expression of collagenase and TIMP-1 in normal and hypertrophic scar.


METHODS
Cell culture and Northern blot hybridization methods were used in this study.


RESULTS
After the disruption of microfilaments with cytochalasin B, mRNA contents of collagenase and TIMP-1 both increased significantly in normal and hypertrophic scar fibroblasts.


CONCLUSIONS
The result suggest that the microfilament cytoskeleton may regulate the synthesis and degradation of ECM, which may be a mechanism of gene regulation during wound healing.
Department of Plastic Surgery, The 85th Hospital of PLA, Shanghai 200052, China.
Haifeng
H
Gu
Qinglian
Q
He
Zihao
Z
Lin
Qi
Q
Liu
Xingmei
X
Zhang
D008841:Actin Cytoskeleton / Q000502:physiology*; D002478:Cells, Cultured; D017364:Collagenases / Q000235:genetics*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics*; D014945:Wound Healing / Q000502:physiology
D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D017364:Collagenases