A complex of the Srb8, -9, -10, and -11 transcriptional regulatory proteins from yeast.
The Srb8, -9, -10, and -11 proteins of yeast have been isolated as a discrete, stoichiometric complex. The isolated complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II at serines 2 and 5. In addition to the previously reported human homologs of Srb10 and 11, we have identified TRAP230/ARC240 and TRAP240/ARC250 as the human homologs of Srb8 and Srb9, showing the entire Srb8/9/10/11 complex is conserved from yeast to humans.
Department of Structural Biology, Stanford University School of Medicine, California 94305-5400, USA.
Tilman
T
Borggrefe
Ralph
R
Davis
Hediye
H
Erdjument-Bromage
Paul
P
Tempst
Roger D
RD
Kornberg
D000595:Amino Acid Sequence; D000818:Animals; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D016213:Cyclins; D019992:Databases as Topic; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D056892:Mediator Complex; D008969:Molecular Sequence Data; D009154:Mutation; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D012319:RNA Polymerase II / Q000737:chemistry* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D035381:TATA-Binding Protein Associated Factors; D013997:Time Factors; D035362:Transcription Factor TFIID; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000378:metabolism*; D019473:Transcription Factors, TFII / Q000378:metabolism; D014158:Transcription, Genetic
D016213:Cyclins; D007074:Immunoglobulin G; D056892:Mediator Complex; C093967:SSN2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C469746:Srb8 protein, S cerevisiae; C490039:TAF6 protein, S cerevisiae; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH; C093345:SSN8 protein, S cerevisiae; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases; C498309:SSN3 protein, S cerevisiae; D012319:RNA Polymerase II