On-line conversion and determination of artemisinin using a flow-injection capillary electrophoresis system.
A novel, rapid, and simple capillary electrophoresis (CE) method has been developed for the determination of antimalarial artemisinin by on-line treatment with alkaline. By on-line reaction, artemisinin was automatically and reproducibly converted to the strongly UV-absorbing compound, Q292, by treating it with 0.20 mol/L NaOH solution for 3 min at 40 degrees C. Analysis was carried out in less than 12 min after conversion of artemisinin in a flow injection (FI) system that was coupled to CE equipment via a split-flow interface cell, and a sampling frequency of 8 h(-1) is achievable. The on-line conversion method has been applied to the determination of artemisinin in the traditional Chinese herbal drug Artemisia annua L., and the results are satisfactory.
Chen
Hong Li
HL
Department of Chemistry, Lanzhou University, Lanzhou, PR China.
Wang
Ke Tai
KT
Pu
Qiao Sheng
QS
Chen
Xing Guo
XG
Hu
Zhi De
ZD
D000962:Antimalarials / Q000032:analysis* / Q000737:chemistry; D031172:Artemisia annua / Q000737:chemistry; D037621:Artemisinins / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004867:Equipment Design; D009862:Online Systems; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D012717:Sesquiterpenes / Q000032:analysis* / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry; D013997:Time Factors
D000962:Antimalarials; D037621:Artemisinins; D010936:Plant Extracts; D012717:Sesquiterpenes; D012972:Sodium Hydroxide; C031327:artemisinin