Peripheral blood mononuclear cell proliferation to heat shock protein-70 derived from autologous lung carcinoma.
In animals, tumor-derived heat shock proteins (HSP) induce immune-mediated protection against autologous cancer. We investigated whether HSP-70 derived from human lung carcinoma are also complexed to tumor-specific antigens. Peripheral blood mononuclear cells collected 10 days after surgery from patients with lung cancer were stimulated with HSP-70 purified from autologous and heterologous tumors. The stimulation index (SI) obtained when stimulating cells with autologous tumor-derived HSP-70 averaged 3.07 +/- 0.75 in patients with lung cancer and 1.57 +/- 0.33 in control subjects (p < 0.001 by analysis of variance). No significant stimulation was observed with HSP-70 derived either from the majority of heterologous tumors or from autologous tumor-free lung tissue. SI decreased from 3.59 +/- 0.65 to 1.65 +/- 0.38 in six patients tested again 3 months after surgery (p = 0.02 by Wilcoxon test for paired data). HSP-70 derived from lung carcinoma are shown to be associated with T cell antigens. The T cell reactivity appears transient and restricted to antigens complexed to HSP-70 derived from autologous tumors only. This suggests that the antigenicity of human lung tumors is unique, which may be crucial for the design of new vaccines.
Chest Department, IRIBHN Statistical Unit, Erasme University Hospital, CUB Erasme, Brussels, Belgium. amichils@ulb.ac.be
Alain
A
Michils
Dominique
D
Dutry
Valérie Zegers
VZ
de Beyl
Myriam
M
Remmelink
Viviane
V
de Maertelaer
Pierre
P
Rocmans
D000230:Adenocarcinoma / Q000473:pathology; D000368:Aged; D000704:Analysis of Variance; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002455:Cell Division / Q000187:drug effects*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000494:pharmacology*; D006801:Humans; D015151:Immunoblotting; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000502:physiology*; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011336:Probability; D012680:Sensitivity and Specificity; D014182:Transplantation, Autologous; D014407:Tumor Cells, Cultured
D018840:HSP70 Heat-Shock Proteins