Effective isolation of magnesium lithospermate B and its inhibition of aldose reductase and fibronectin on mesangial cell line.
We developed an effective isolation method of magnesium lithospermate B from Salviae miltiorrhizae Radix and found for the first time that magnesium lithospermate B shows strong in vitro inhibition (IC50=0.04 microM) of aldose reductase (AR), 2.5 times than that of clinically used epalrestat (IC50=0.1 microM) and accumulation of fibronectin dose dependently.
Jung
Mankil
M
Department of Chemisty, Yonsei University, Seoul, Korea. mkjung@alchemy.yonsei.ac.kr
Lee
Hyun Chul
HC
Ahn
Chul Woo
CW
Park
Whuijung
W
Choi
Sunhee
S
Kim
Hanjo
H
Cho
Donghak
D
Lee
Geun Taek
GT
Li
Hai-Ri
HR
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005353:Fibronectins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D051379:Mice; D010936:Plant Extracts; D018517:Plant Roots / Q000737:chemistry; D027561:Salvia miltiorrhiza
D004365:Drugs, Chinese Herbal; D005353:Fibronectins; D010936:Plant Extracts; C061091:lithospermate B; D000449:Aldehyde Reductase