Amorphous solid water produced by cryosectioning of crystalline ice at 113 K.
Amorphous solid (vitreous) water can be obtained by a number of methods, including quick freezing of a very small volume of pure water, low pressure condensation of water vapour on a cold substrate or transformation of hexagonal ice (the ice which is naturally formed) under very high pressure at liquid nitrogen temperature. Larger volumes can be vitrified if cryoprotectant is added or when samples are frozen under high pressure. We show that a sample of 17.5% dextran solution or mouse brain tissue, respectively, frozen under high pressure (200 MPa) into cubic or hexagonal ice can be transformed into vitreous water by the very process of cryosectioning. The vitreous sections obtained by this procedure differ from cryosections obtained from vitreous samples by the irregular aspect of the sections and by small but significant differences in the electron diffraction patterns. For the growing community of cryo-ultramicrotomists it is important to know that vitrification can occur at the knife edge. A vitreous sample is considered to show the best possible structural preservation. The sort of vitrification described here, however, can lead to bad structural preservation and is therefore considered to be a pitfall. Furthermore, we compare these sections with other forms of amorphous solid water and find it similar to high density amorphous water produced at very high pressures (about 1 GPa) from hexagonal ice and annealed close to its transformation temperature at 117 K.
Laboratoire d'Analyse Ultrastructurale, Bâtiment de Biologie, Université de Lausame, CH-1015, Lausame, Switzerland.
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AL-Amoudi
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Dubochet
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Studer
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D016616:Cryoultramicrotomy / Q000379:methods*; D003460:Crystallization; D007053:Ice; D051379:Mice; D014867:Water / Q000737:chemistry*
D007053:Ice; D014867:Water