Ultrastructural and biophysical studies on protein conformations of epithelium and stroma in benign prostatic hyperplasia before and after transurethral resection of the prostate.
The alterations in protein conformation of epithelium and stroma in human benign prostatic hyperplasia (BPH) undergoing prostatic needle biopsy (PNB) and transurethral resection of the prostate (TURP) were isolated in vitro and investigated using attenuated total reflectance (ATR)/Fourier transform infrared (FT-IR) microspectroscopy. Second-derivative spectral analysis and a curve-fitting program were used to verify and obtain the protein secondary conformation and composition of the samples. TURP uses an electric cutting heat but PNB is a nonheating process. The results indicate a predominantly higher proportion of beta-sheet conformation of the epithelium or stroma in BPH tissue at 1633 or 1630 cm(-1) in the amide I band, but after treatment with TURP, both maximum peaks shifted to 1644 or 1646 cm(-1) due to random coil structure, respectively. This conversion suggests that the heat generated by the cutting loop could increase the temperature in the tissue surrounding the prostate, thereby modifying the protein conformational structure of epithelium and stroma in BPH tissue.
Division of Urology, Lotung Pohai Hospital, Ilan, Taiwan, Republic of China.
Hueih-Shing
HS
Hsu
Shan-Yang
SY
Lin
Mei-Jane
MJ
Li
Run-Chu
RC
Liang
D000368:Aged; D001707:Biopsy, Needle; D004848:Epithelium / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D008297:Male; D011467:Prostate / Q000737:chemistry* / Q000473:pathology* / Q000601:surgery / Q000648:ultrastructure; D011470:Prostatic Hyperplasia / Q000473:pathology* / Q000601:surgery; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011506:Proteins / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D017154:Stromal Cells / Q000737:chemistry / Q000648:ultrastructure; D020728:Transurethral Resection of Prostate / Q000009:adverse effects*
D011506:Proteins