Comparison of the prevention of aflatoxin b(1)-induced genotoxicity by quercetin and quercetin pentaacetate.
Earlier work carried out in our laboratory highlighted the mode of action of acetoxy 4-methylcoumarins in preventing the genotoxicity of aflatoxin B(1) (AFB(1)). We have in this report extended the observations to quercetin pentaacetate (QPA), which unlike quercetin (Q) has demonstrated time-dependent inhibition of liver microsome catalysed AFB(1) epoxidation as measured by AFB(1) binding to DNA. The action of QPA is similar to that of the acetoxy 4-methylcoumarins in that they are acted upon by microsomal transacetylase leading to modulation of catalytic activities of certain enzymes (such as P-450 enzymes, NADPH cytochrome C reductase and glutathione S-transferase) possibly by way of protein acetylation. In the present work, we have documented the transacetylase-mediated action of QPA in preventing genotoxicity due to AFB(1).
Kohli
Ekta
E
Department of Biochemistry, V. P. Chest Institute, University of Delhi, -110 007, Delhi, India.
Raj
Hanumantharao G
HG
Kumari
Ranju
R
Rohil
Vishwajit
V
Kaushik
Narendra K
NK
Prasad
Ashok K
AK
Parmar
Virinder S
VS
D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000648:ultrastructure; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D008297:Male; D015162:Micronucleus Tests; D008862:Microsomes, Liver / Q000201:enzymology; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D016587:Antimutagenic Agents; D003577:Cytochrome P-450 Enzyme System; D011794:Quercetin; D016604:Aflatoxin B1; D009251:NADPH-Ferrihemoprotein Reductase; C435940:quercetin pentaacetate