DNA microarray technology used for studying foodborne pathogens and microbial habitats: minireview.
Microarray analysis is an emerging technology that has the potential to become a leading trend in bacterial identification in food and feed improvement. The technology uses fluorescent-labeled probes amplified from bacterial samples that are then hybridized to thousands of DNA sequences immobilized on chemically modified glass slides. The whole gene or open reading frame(s) is represented by a polymerase chain reaction fragment of double-strand DNA, approximately 1000 base pair (bp) or 20-70 bp single-strand oligonucleotides. The technology can be used to identity bacteria and to study gene expression in complex microbial populations, such as those found in food and gastrointestinal tracts. Data generated by microarray analysis can be potentially used to improve the safety of our food supply as well as ensure the efficiency of animal feed conversion to human food, e.g., in meat and milk production by ruminants. This minireview addresses the use of microarray technology in bacterial identification and gene expression in different microbial systems and in habitats containing mixed populations of bacteria.
U.S. Food and Drug Administration, CFSAN, Division of Microbiological Studies, College Park, MD 20740-3855, USA. Sufian.Al-Khaldi@cfsan.fda.gov
Sufian F
SF
Al-Khaldi
Scott A
SA
Martin
Avraham
A
Rasooly
Jeff D
JD
Evans
D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004777:Environment; D005516:Food Microbiology; D005798:Genes, Bacterial; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012417:Rumen / Q000382:microbiology
D004269:DNA, Bacterial