Cell adhesion behavior of chitosan surface modified by bonding 2-methacryloyloxyethyl phosphorylcholine.
2-Methacryloyloxyethyl phosphorylcholine (MPC)-bonded chitosan was prepared by Michael addition of MPC to the amino groups of chitosan. The modified surfaces were characterized by static contact angle and electron spectroscopy for chemical analysis (ESCA). The water contact angle of chitosan decreased with the MPC bonding and the rate of decrease depended on the amount of MPC bonding. ESCA analysis results proved that MPC had been bonded on the chitosan surface and the chitosan modified directly by MPC had a much higher concentration of MPC on the surface compared with that of MPC on chitosan modified indirectly by MPC. Cell adhesion tests indicated that a low concentration of MPC bonded chitosan was more favorable to cell adhesion while a high concentration of MPC bonded chitosan inhibited cell attachment.
Department of Polymer Science and Engineering, Nanjing University, PR China.
Aiping
A
Zhu
Shiquan
S
Wang
Youling
Y
Yuan
Jian
J
Shen
D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism; D048271:Chitosan; D005347:Fibroblasts / Q000166:cytology*; D008689:Methacrylates / Q000737:chemistry* / Q000494:pharmacology; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects
D008689:Methacrylates; D010767:Phosphorylcholine; D002686:Chitin; C070638:2-methacryloyloxyethyl phosphorylcholine; D048271:Chitosan