Preparation and analysis of small unilamellar phospholipid vesicles of a uniform size.
The interaction of carbonmonoxyhemoglobin and heme with small unilamellar phospholipid vesicles was studied using dynamic light scattering. Addition of carbonmonoxyhemoglobin to dimyristoylphosphatidylcholine:dimyristoylphosphatidylserine small unilamellar vesicles resulted in an increase of average vesicle size from 17.4 to 32.0nm. Addition of heme to vesicles produced a smaller size increase, from 17.4 to 21.0nm. Also reported is a method for preparing small unilamellar lipid vesicles of a uniform size, suitable for use in NMR spectroscopy.
Department of Chemistry, Stanford University, 94305, Stanford, CA, USA. pitcher@niehs.nih.gov
Wayne H
WH
Pitcher
Wray H
WH
Huestis
D002263:Carboxyhemoglobin / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000032:analysis; D006418:Heme / Q000737:chemistry; D008027:Light; D008081:Liposomes / Q000737:chemistry*; D010718:Phosphatidylserines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D012542:Scattering, Radiation
D008081:Liposomes; D010718:Phosphatidylserines; D010743:Phospholipids; D006418:Heme; C042859:dimyristoylphosphatidylserine; D002263:Carboxyhemoglobin; D004134:Dimyristoylphosphatidylcholine