Characterization of the complexes of furosemide with 2-hydroxypropyl-beta-cyclodextrin and sulfobutyl ether-7-beta-cyclodextrin.
The purpose of this study was to prepare and characterize complexes of furosemide with 2-hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD) and sulfobutyl ether-7-beta-cyclodextrin (SBE-7-beta-CD). Solid complexes of furosemide with 2-HP-beta-CD and SBE-7-beta-CD were prepared by using both a freeze-drying and kneading method. Physical mixtures were prepared for comparison. The inclusion complexes were characterized by differential scanning calorimetry, X-ray diffractometry (XRD) and 1H nuclear magnetic resonance spectroscopy (1H-NMR). 1H-NMR, and especially the use of the two-dimensional ROESY spectrum, was used to determine the position of the furosemide molecule inside the cyclodextrin cavity. 1H-NMR studies showed that furosemide fit into the cyclodextrin torus cavity with its furane ring nearest to the primary hydroxyl side.
Spamer
Elisma
E
Department of Pharmaceutics, School of Pharmacy, Potchefstroom University for Christian Higher Education, 2520, Potchefstroom, South Africa .
Müller
Douw G
DG
Wessels
Philippus L
PL
Venter
Johannes P
JP
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D002152:Calorimetry, Differential Scanning; D003505:Cyclodextrins / Q000737:chemistry*; D004339:Drug Compounding; D005612:Freeze Drying; D005665:Furosemide / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015394:Molecular Structure; D012995:Solubility; D014961:X-Ray Diffraction; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; C093196:SBE4-beta-cyclodextrin; D005665:Furosemide