Diagnostic techniques to detect cryptic leishmaniasis in dogs.
This study of several techniques for detecting cryptic leishmaniasis in dogs from areas in Spain where Leishmania infantum is highly endemic concludes that immunological techniques (enzyme-linked immunosorbent assay, immunofluorescence antibody test, Western blotting, delayed-type hypersensitivity reaction, and in vitro lymphocyte proliferation assay) do not clearly differentiate between noninfected and infected asymptomatic dogs and that culture and PCR are more reliable diagnostic tools.
Laboratori de Parasitologia, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain.
Laura
L
Iniesta
Salceda
S
Fernández-Barredo
Béatrice
B
Bulle
M Teresa
MT
Gómez
Renaud
R
Piarroux
Montserrat
M
Gállego
José M
JM
Alunda
Montserrat
M
Portús
D000818:Animals; D015153:Blotting, Western; D002353:Carrier State; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006968:Hypersensitivity, Delayed / Q000175:diagnosis / Q000469:parasitology; D018314:Leishmania infantum / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000175:diagnosis* / Q000276:immunology