Vesicles in the subacrosomal space and partial diaphragms in the subacrosomal nuclear envelope of round spermatids of a rat injected intravenously with gold labeled-testosterone-bovine serum albumin conjugate: vesicular trafficking from acrosome to nucleus.
Colloidal gold labeled-testosterone-bovine serum albumin conjugate (testosterone-BSA-gold) injected into the vascular system of rats is taken up by endocytosis into round spermatids. Based on observation of silver deposits indicating testosterone-BSA-gold with silver enhancement, we have suggested that testosterone-BSA-gold enters the nuclei through not only the postacrosomal nuclear envelope but also the subacrosomal nuclear envelope (SNE) via the acrosome (Nishimura and Nakano, 1997). However, it was unclear how testosterone-BSA-gold in the acrosome entered the nucleoplasm. Spermatids showing silver deposits on the subacrosomal space were observed under electron microscope without silver enhancement, to clarify the courses of translocation. In the spermatids, vesicles with the gold particles were seen in the subacrosomal space. Some of the vesicles were in contact with the SNE. A part of the outer nuclear membrane projected into the space. Furthermore, local single-bilayer nuclear membranes, which seemed to partially lack nuclear lamina, were present in the SNE. These results indicate the possibility that the vesicles mediate the transport of testosterone-BSA-gold from acrosome to nucleus, and that the vesicle membrane fuses with not only the outer nuclear membrane but also a shared bilayer in the SNE.
Nishimura
Toshikazu
T
Department of Anatomy, Aichi Medical University School of Medicine, Yazako, Japan. nishimur@aichi-med-u.ac.jp
Nakano
Takashi
T
D000177:Acrosome / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D002417:Cattle; D017979:Gold Colloid; D007275:Injections, Intravenous; D008297:Male; D009685:Nuclear Envelope / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D013087:Spermatids / Q000378:metabolism*; D013739:Testosterone / Q000008:administration & dosage / Q000493:pharmacokinetics*; D022161:Transport Vesicles / Q000378:metabolism* / Q000648:ultrastructure
D017979:Gold Colloid; C045036:testosterone-15 beta-bovine serum albumin conjugate; D012710:Serum Albumin, Bovine; D013739:Testosterone