[Experimental study on bacteriostasis of chitosan and sodium hyaluronate].
OBJECTIVE
To compare the effect and coverage of bacteriostasis of chitosan and sodium hyaluronate.


METHODS
Each of the five bacteria, Proteus mirabilis, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, Staphylococcus aureus, was cultivated for 33 tubes of broth culture. Leaving three tubes each group as control group, ploidy diluted concentration of high relative molecular weight chitosan, low relative molecular weight chitosan and sodium hyaluronate were added respectively in the broth culture. All the tubes were cultivated for 18 hours at 37 degrees C with homeothermia. Then the growth of bacteria was observed.


RESULTS
The minimal inhibitory concentrations (MIC) of high relative molecular weight chitosan were: Proteus mirabilis 0.031%, Escherichia coli 0.063%, Candida albicans 0.063%, Pseudomonas aeruginosa 0.063%, Staphylococcus aureus 0.063%; and the MIC of low relative molecular weight chitosan were: Proteus mirabilis 0.125%, Escherichia coli 0.025%, Candida albicans 0.25%, Pseudomonas aeruginosa 0.25%, Staphylococcus aureus 0.125%; bacteria grew well in each tube of sodium hyaluronate group and control group.


CONCLUSIONS
The above results show that sodium hyaluronate has no bacteriostasis, while chitosan has bacteriostasis on broad spectrum and high relative molecular weigh: chitosan has stronger effect.
Department of Orthopedic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, P. R. China 200003.
Zhao-hui
ZH
Tang
Chun-lin
CL
Hou
Qing-quan
QQ
Chen
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D002686:Chitin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000254:growth & development; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D002686:Chitin; D006820:Hyaluronic Acid