Effect of vaccinations with recombinant fusion proteins on Ancylostoma caninum habitat selection in the canine intestine.
Laboratory dogs were vaccinated subcutaneously with 3 different recombinant fusion proteins, each precipitated with alum or calcium phosphate. The vaccinated dogs were then challenged orally with 400 third-stage infective larvae (L3) of the canine hookworm, Ancylostoma caninum. The 3 A. caninum antigens selected were Ac-TMP, an adult-specific secreted tissue inhibitor of metalloproteases; Ac-AP, an adult-specific secreted factor Xa serine protease inhibitor anticoagulant; and Ac-ARR-1, a cathepsin D-like aspartic protease. Each of the 3 groups comprised 6 male beagles (8 +/- 1 wk of age). A fourth group comprised control dogs injected with alum. All of the dogs vaccinated with Ac-TMP or Ac-APR-1 exhibited a vigorous antigen-specific antibody response, whereas only a single dog vaccinated with Ac-AP developed an antibody response. Dogs with circulating antibody responses exhibited 4.5-18% reduction in the numbers of adult hookworms recovered from the small intestines at necropsy, relative to alum-injected dogs. In contrast, there was a concomitant increase in the number of adult hookworms recovered from the colon. The increase in colonic hookworms was as high as 500%, relative to alum-injected dogs. Female adult hookworms were more likely to migrate into the colon than were males. Anti-enzyme and anti-enzyme inhibitor antibodies correlated with an alteration in adult hookworm habitat selection in the canine gastroinntestinal tract.
Hotez
Peter J
PJ
Department of Microbiology and Tropical Medicine, Sabin Vaccine Institute, George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037, USA.
Ashcom
James
J
Bin
Zhan
Z
Bethony
Jeffrey
J
Williamson
Angela
A
Hawdon
John M
JM
Jianjun
Feng
F
Dobardzic
Azra
A
Rizo
Ivania
I
Bolden
Janelle
J
Jin
Qun
Q
Yan
Wang
W
Dobardzic
Reshad
R
Chung-Debose
Sophia
S
Crowell
Melissa
M
Datu
Bennett
B
Delaney
Angela
A
Dragonovski
Dilyan
D
Jiang
Yang
Y
Yueyuan
Liu
L
Ghosh
Kashinath
K
Loukas
Alex
A
Brandt
Walter
W
Russell
Philip K
PK
Zook
Bernard C
BC
D000722:Ancylostoma; D000724:Ancylostomiasis / Q000276:immunology* / Q000469:parasitology; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000008:administration & dosage* / Q000276:immunology; D004285:Dogs; D005260:Female; D007422:Intestines / Q000469:parasitology; D008297:Male; D010270:Parasite Egg Count; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage* / Q000276:immunology; D014611:Vaccination
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011993:Recombinant Fusion Proteins