Prospective survey of beta-lactamases produced by ceftazidime- resistant Pseudomonas aeruginosa isolated in a French hospital in 2000.
In 2000, at the Université d'Auvergne teaching hospital in Clermont-Ferrand, France, 44 (6.2%) strains of Pseudomonas aeruginosa were found to be resistant to ceftazidime. After genotyping, 34 strains were selected. Nine had an additional beta-lactamase: OXA-21 (n = 6), PSE-1 (CARB-2) (n = 2), or PER-1 (n = 1). Ceftazidime resistance was related solely to the overproduction of the cephalosporinase in 30 strains. Sequencing of five bla(AmpC) genes encoding cephalosporinases with different pIs showed 99% identity with the ampC gene of P. aeruginosa PAO1.
De Champs
Christophe
C
Service de Bactériologie, Faculté de Médecine, Université d'Auvergne, 63001 Clermont-Ferrand, France. christophe.dechamps@u-clermont1.fr
Poirel
Laurent
L
Bonnet
Richard
R
Sirot
Danielle
D
Chanal
Catherine
C
Sirot
Jacques
J
Nordmann
Patrice
P
D002442:Ceftazidime / Q000494:pharmacology* / Q000627:therapeutic use; D002510:Cephalosporinase / Q000235:genetics / Q000378:metabolism; D002511:Cephalosporins / Q000494:pharmacology* / Q000627:therapeutic use; D003625:Data Collection; D004352:Drug Resistance, Microbial; D005602:France / Q000453:epidemiology; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D011446:Prospective Studies; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology*; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D002511:Cephalosporins; D065093:beta-Lactamase Inhibitors; D002442:Ceftazidime; D002510:Cephalosporinase; D001618:beta-Lactamases