Molecular characterization of multiresistant d-tartrate-positive Salmonella enterica serovar paratyphi B isolates.
Since 1996, the National Salmonella Reference Laboratory of Germany has received an increasing number of Salmonella enterica subsp. enterica serovar Paratyphi B isolates. Nearly all of these belonged to the dextrorotatory tartrate-positive variant (S. enterica subsp. enterica serovar Paratyphi B dT(+)), formerly called S. enterica subsp. enterica serovar Java. A total of 55 selected contemporary and older S. enterica subsp. enterica serovar Paratyphi B dT(+) isolates were analyzed by plasmid profiling, antimicrobial resistance testing, pulsed-field gel electrophoresis, IS200 profiling, and PCR-based detection of integrons. The results showed a high genetic heterogeneity among 10 old strains obtained from 1960 to 1993. In the following years, however, new distinct multiresistant S. enterica subsp. enterica serovar Paratyphi B dT(+) clones emerged, and one clonal lineage successfully displaced the older ones. Since 1994, 88% of the isolates investigated were multiple drug resistant. Today, a particular clone predominates in some German poultry production lines, poultry products, and various other sources. It was also detected in contemporary isolates from two neighboring countries as well.
Miko
Angelika
A
National Salmonella Reference Laboratory, Federal Institute for Health Protection of Consumers and Veterinary Medicine, 12277 Berlin, Germany.
Guerra
Beatriz
B
Schroeter
Andreas
A
Dorn
Christina
C
Helmuth
Reiner
R
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D004251:DNA Transposable Elements; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005858:Germany; D019426:Integrases / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011200:Poultry; D011201:Poultry Diseases / Q000382:microbiology; D011202:Poultry Products / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000382:microbiology; D019779:Salmonella enterica / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012703:Serotyping; D013644:Tartrates / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D013644:Tartrates; D019426:Integrases