Clinical isolates of Staphylococcus aureus with ribosomal mutations conferring resistance to macrolides.
Six strains of Staphylococcus aureus isolated from cystic fibrosis patients after treatment with azithromycin were cross-resistant to azithromycin and erythromycin. None of the isolates contained erm or msr(A) genes, but they all carried either A2058G/U or A2059G mutations within the rrl genes, with a majority of the rRNA copies bearing the mutation. One strain displayed an additional mutation in the rplV gene, encoding the L22 ribosomal protein.
Service de Microbiologie, CHU Côte de Nacre, Caen, France.
Anne-Laure
AL
Prunier
Brigitte
B
Malbruny
Didier
D
Tandé
Bertrand
B
Picard
Roland
R
Leclercq
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D003550:Cystic Fibrosis / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D004351:Drug Resistance; D004917:Erythromycin / Q000494:pharmacology; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D026901:Membrane Transport Proteins; D008780:Methyltransferases; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics*; D015345:Oligonucleotide Probes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012270:Ribosomes / Q000235:genetics*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; D015345:Oligonucleotide Probes; C066202:msrA protein, Staphylococcus epidermidis; D004917:Erythromycin; D017963:Azithromycin; D008780:Methyltransferases; C431709:ErmA protein, Bacteria