Genetic characterization of dengue virus type 3 isolates in the State of Rio de Janeiro, 2001.
The genetic characterization of dengue virus type 3 (DEN-3) strains isolated from autochthonous cases in the State of Rio de Janeiro, Brazil, in 2001 is presented. Restriction site-specific (RSS)-PCR performed on 22 strains classified the Brazilian DEN-3 viruses as subtype C, a subtype that contains viruses from Sri Lanka, India, Africa and recent isolates from Central America. Nucleic acid sequencing (positions 278 to 2550) of one DEN-3 strain confirmed the origin of these strains, since genotype III - classified by sequencing - and RSS-PCR subtype C are correlated. This genetic subtype has been associated with hemorrhagic dengue epidemics and the information provided here could be useful to implement appropriate prevention and control measures.
Departamento de Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil. marizepm@ioc.fiocruz.br
M P
MP
Miagostovich
F B
FB
dos Santos
T S
TS
de Simone
E V
EV
Costa
A M B
AM
Filippis
H G
HG
Schatzmayr
R M R
RM
Nogueira
D001938:Brazil; D030561:Databases, Nucleic Acid; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping