The effect of benzalkonium chloride on rabbit nasal mucosa in vivo: an electron microscopy study.
In this study we investigated the histopathological changes of nasal mucosa after the use of topical benzalkonium-chloride solution. In the study, 28 male New Zealand white rabbits were used. The animals were divided into two groups. In the first group, 50 micro l of 0.001% benzalkonium-chloride solution was sprayed into the right nostril and 50 micro l of 0.9% saline into the left twice daily for 14 days. In the second group, the same solutions were applied twice daily for 28 days. All of the biopsies from each nasal cavity were processed for electron microscopy. In the nostrils treated with 0.001% benzalkonium chloride, there were areas with obvious squamous cell metaplasia. Some cells showed broken cristae of the mitochondria. Degenerative changes in the supportive and olfactory cells, deformation of nuclei and the increase of heterochromatin were observed. The present investigation has shown that the application of topical benzalkonium chloride does induce degenerative morphologic changes in the nasal mucosa in rabbits in vivo.
Dicle University School of Medicine, Department of Otorhinolaryngology, 21280 Diyarbakir, Turkey.
Sebahattin
S
Cüreoğlu
Murat
M
Akkuş
Ustün
U
Osma
Mehmet
M
Yaldiz
Faruk
F
Oktay
Belgin
B
Can
Cengiz
C
Güven
Muhammet
M
Tekin
Faruk
F
Meriç
D000287:Administration, Topical; D000818:Animals; D001548:Benzalkonium Compounds / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008297:Male; D008679:Metaplasia / Q000139:chemically induced / Q000473:pathology; D008854:Microscopy, Electron; D009297:Nasal Mucosa / Q000187:drug effects* / Q000648:ultrastructure*; D011310:Preservatives, Pharmaceutical / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D011817:Rabbits
D001548:Benzalkonium Compounds; D011310:Preservatives, Pharmaceutical