Capillary magnetophoresis of human blood cells and their magnetophoretic trapping in a flow system.
The performance of a capillary magnetophoretic device was improved by enhancing the magnetic field gradient using a pair of small iron tips attached to the Nd-Fe-B magnets. The magnetophoretic intensity, B(dB/dx), was determined as a function of distance along the gap between the tips from the magnetophoretic velocity of a 3 microm polystyrene microparticle in 0.6 M manganese(II) chloride solution. The maximum intensity was increased 4.5 times by the attached iron pieces. The magnetophoresis of a single human blood cell in 0.1 M manganese(II) solution was studied by this method and its magnetic susceptibility was estimated. Magnetophoretic trapping of red blood cells was demonstrated under counter-current flow conditions in the capillary.
Department of Chemistry, Graduate School of Science, Osaka University, Machikaneyama, Toyonaka, Japan. watarai@chem.sci.osaka-u.ac.jp
Hitoshi
H
Watarai
Makoto
M
Namba
D002469:Cell Separation; D019075:Electrophoresis, Capillary / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D008280:Magnetics