Fluorescein-based pI markers for capillary isoelectric focusing with laser-induced fluorescence detection.
We prepared a series of low-molecular-mass fluorescent ampholytes with narrow pI range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent pI markers, were examined. The pI values of new fluorescein-based pI markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass pI markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented.
Slais
Karel
K
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veverí 97, CZ-61142 Brno, Czech Republic. slais@iach.cz
Horká
Marie
M
Novácková
Jana
J
Friedl
Zdenek
Z
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods; D019793:Fluorescein; D016650:Fluorescein-5-isothiocyanate; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D007834:Lasers; D009994:Osmolar Concentration; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D012015:Reference Standards
D005456:Fluorescent Dyes; D011506:Proteins; D016650:Fluorescein-5-isothiocyanate; D019793:Fluorescein