Mechanochemical ATPases and transcriptional activation.
Transcriptional activator proteins that act upon the sigma54-containing form of the bacterial RNA polymerase belong to the extensive AAA+ superfamily of ATPases, members of which are found in all three kingdoms of life and function in diverse cellular processes, often via chaperone-like activities. Formation and collapse of the transition state of ATP for hydrolysis appears to engender the interaction of the activator proteins with sigma54 and leads to the protein structural transitions needed for RNA polymerase to isomerize and engage with the DNA template strand. The common oligomeric structures of AAA+ proteins and the creation of the active site for ATP hydrolysis between protomers suggest that the critical changes in protomer structure required for productive interactions with sigma54-holoenzyme occur as a consequence of sensing the state of the gamma-phosphate of ATP. Depending upon the form of nucleotide bound, different functional states of the activator are created that have distinct substrate and chaperone-like binding activities. In particular, interprotomer ATP interactions rely upon the use of an arginine finger, a situation reminiscent of GTPase-activating proteins.
Zhang
X
X
Imperial College Centre for Structural Biology, Imperial College of Science, Technology and Medicine, London, UK.
Chaney
M
M
Wigneshweraraj
Siva R
SR
Schumacher
J
J
Bordes
P
P
Cannon
W
W
Buck
M
M
D000251:Adenosine Triphosphatases / Q000502:physiology*; D000595:Amino Acid Sequence; D001704:Biopolymers; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D051877:RNA Polymerase Sigma 54; D017386:Sequence Homology, Amino Acid; D012808:Sigma Factor / Q000378:metabolism; D015533:Transcriptional Activation / Q000502:physiology*
D001704:Biopolymers; D004268:DNA-Binding Proteins; D012808:Sigma Factor; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54; D000251:Adenosine Triphosphatases