Lumin, a cyanine dye, enhances interleukin 12-dependent interferon gamma production by lipopolysaccharide-stimulated mouse splenocytes.
Lumin was orally administered to mice daily for 3 d, and on the day following the final administration, mice were sacrificed and splenocytes were stimulated with lipopolysaccharide (LPS). Splenocytes obtained from lumin-treated mice showed enhanced production of interferon gamma (IFN-gamma) and increased percentages of CD3+ cells. Although T cells are considered to be the source of IFN-gamma, it is unlikely that LPS directly stimulates T cells. Next we performed neutralization experiments using a monoclonal antibody (mAb) against interleukin (IL-)12 because this cytokine, which is produced by macrophages, has the direct ability to induce IFN-gamma production and the proliferation of activated T cells. This antibody inhibited IFN-gamma production by splenocytes. We thus show that orally administered lumin enhances IFN-gamma production by splenocytes when the latter are stimulated with LPS, a phenomenon that was observed in correlation with activation of T cells by IL-12, that is produced by macrophages.
Kunikata
Toshio
T
Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc, Okayama, Japan. fujisaki@hayashibara.co.jp
Ishihara
Tatsuya
T
Ushio
Shimpei
S
Iwaki
Kanso
K
Ikeda
Masao
M
Kurimoto
Masashi
M
D000818:Animals; D004357:Drug Synergism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011806:Quinolinium Compounds / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D005456:Fluorescent Dyes; D008070:Lipopolysaccharides; D011806:Quinolinium Compounds; D018664:Interleukin-12; C052194:lumin; D007371:Interferon-gamma