Simultaneous analysis of monosaccharides and oligosaccharides by high-performance liquid chromatography with postcolumn fluorescence derivatization.
To develop a fluorimetric HPLC technique for the simultaneous microanalysis of reducing mono- and oligosaccharides, the technique of linear gradient elution was introduced into the postcolumn fluorimetric detemination system of reducing saccharides with benzamidine. Fluorescence measurement was performed at 288 nm for excitation and 470 nm for emission and an optimization study for this postcolumn fluorescence derivatization carried out. Under optimum conditions, the detection limits of D-glucose and maltohexaose were 1.78 and 2.59 pmol, respectively. The present method was successfully applied to saccharide analysis and should prove useful for automated simultaneous microanalysis of reducing mono- and oligosaccharides in foods.
Kakita
Hirotaka
H
AIST Shikoku, National Institute of Advanced Industrial Science and Technology, Takamatsu, Kagawa, Japan. h-kakita@aist.go.jp
Kamishima
Hiroshi
H
Komiya
Katsuo
K
Kato
Yoshio
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009005:Monosaccharides / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D009005:Monosaccharides; D009844:Oligosaccharides