[Hemopoiesis on stromal sublayers formed by constant lines of fibroblasts].
We studied the formation of hemopoietic colonies on an artificial sublayer in the peritoneal cavity of mice under the influence of stromal sublayers consisting of fibroblasts of six constant cell limes. All studied lines of stromal cells supported the formation of granulocytic foci and some of them supported the formation of erythroid foci as well. It was shown that hemopoiesis was preserved or, in some cases, enhanced on sublayers of fixed (metabolically inactive) cells. The treatment of fibroblasts by E. coli lipopolysaccharide did not lead, as a rule, to significant stimulation of hemopoiesis.
Michurina
T V
TV
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 117808.
Paiushina
O V
OV
Bueverova
E I
EI
Satdykova
G P
GP
Bragina
E V
EV
Nikonova
T M
TM
Khrushchov
N G
NG
D016475:3T3 Cells; D000818:Animals; D001773:Blood Cells / Q000166:cytology* / Q000187:drug effects; D002460:Cell Line; D017690:Cell Transplantation; D016207:Cytokines / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005976:Glutaral / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology*; D051381:Rats; D017154:Stromal Cells
D016207:Cytokines; D008070:Lipopolysaccharides; D005976:Glutaral