Leptotrichia amnionii sp. nov., a novel bacterium isolated from the amniotic fluid of a woman after intrauterine fetal demise.
A novel bacterium was isolated and characterized from the amniotic fluid of a woman who experienced intrauterine fetal demise in the second trimester of pregnancy. The bacterium was a slow-growing, gram-negative anaerobic coccobacillus belonging to the genus LEPTOTRICHIA: Unlike Leptotrichia sanguinegens, the isolate did not grow in chopped-meat glucose broth or on sheep blood agar upon subculturing. The isolate was characterized by sequencing and analyzing its 16S rRNA gene. The 1,493-bp 16S ribosomal DNA sequence had only 96% homology with L. sanguinegens. Several phylogenetic analyses indicated that L. amnionii is a distinct species and most closely related to L. sanguiegens.
Clinical Research Center, Marshfield Medical Research and Education Foundation, Wisconsin 54449, USA. shuklas@mmrf.mfldclin.edu
Sanjay K
SK
Shukla
Paul R
PR
Meier
Paul D
PD
Mitchell
Daniel N
DN
Frank
Kurt D
KD
Reed
D000328:Adult; D000653:Amniotic Fluid / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D005313:Fetal Death; D006089:Gram-Negative Anaerobic Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D011262:Pregnancy Trimester, Second; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S