Use of a CO2 electrode to monitor fermentations that use xenobiotic compounds as source of carbon.
Bacterial strains were isolated from contaminated waters, mud or soils. They are capable of growing in mineral medium with different chemicals as carbon source, such as aliphatic or aromatic hydrocarbons and polychlorinated biphenyls (PCBs). Most of these strains tolerate high concentrations (up to 30% v/v) of the xenobiotic substrates. This is particularly important for the development of fermenting processes to treat effluents or residues with a high content of contaminating compounds. An ion-specific potentiometric electrode (CO2) has been developed to measure CO2 production continuously. When the different strains were incubated in a mineral medium and in the presence of the corresponding substrate, a parallel between growth, substrate consumption and CO2 production was found. The developed system is suggested as an efficient and economical alternative to evaluate the potential of biodegradation by different microorganisms.
Laboratorio de Microbiología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, 4o Piso, Ciudad Universitaria, 1428 Buenos Aires, Argentina.
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D000421:Alcaligenes / Q000302:isolation & purification / Q000378:metabolism*; D000473:Alkanes / Q000378:metabolism; D001140:Aroclors / Q000378:metabolism; D001673:Biodegradation, Environmental; D002138:Calibration; D002244:Carbon / Q000378:metabolism*; D002245:Carbon Dioxide / Q000032:analysis* / Q000378:metabolism; D004566:Electrodes; D004785:Environmental Pollutants / Q000378:metabolism; D004867:Equipment Design; D005285:Fermentation; D006838:Hydrocarbons / Q000378:metabolism*; D008837:Micrococcus / Q000302:isolation & purification / Q000378:metabolism*; D011199:Potentiometry / Q000295:instrumentation*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D020058:Styrene / Q000378:metabolism; D014050:Toluene / Q000378:metabolism; D014871:Water Microbiology; D014877:Water Pollution, Chemical; D015262:Xenobiotics / Q000378:metabolism*
D000473:Alkanes; D001140:Aroclors; D004785:Environmental Pollutants; D006838:Hydrocarbons; D015262:Xenobiotics; D002245:Carbon Dioxide; D014050:Toluene; D020058:Styrene; D002244:Carbon; C012867:decane