[Plasmatic dosage of antiretroviral drugs by high performance liquid chromathography ].
A high-performance liquid chromatographic method has been developed for the determination of eight antiretroviral drugs (amprenavir, indinavir, lopinavir, nelfinavir, ritonavir, saquinavir, efavirenz and nevirapine) in a single run. After a liquid-liquid extraction with diethylether, the antiretroviral drugs are separated on a Stability RP18 column eluted with a gradient of acetonitrile and phosphate buffer (50 mM pH 5.65). A sequential ultraviolet detection allowed for simultaneous quantitation of antiretroviral drugs (240, 215, 260 nm). Calibration curves were linear in the range 100-10,000 ng/ml. The limit of quantitation was 50 ng/ml for all drugs except for nevirapine (100 ng/ml). The accuracies ranged from 88.2% to 110.9% and both inter- and intra-day coefficients of variation were lower than 11%. The extraction recoveries were higher than 62%. This method is simple and shows good specificity with respect to commonly coprescribed drugs.
Laboratoire de Pharmacologie Clinique et de Toxicologie, Hôpital Pellegrin et Université Victor Ségalen, Bordeaux, France.
K
K
Titier
F
F
Lagrange
F
F
Péhourcq
L
L
Edno-Mcheik
N
N
Moore
M
M
Molimard
D019380:Anti-HIV Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D016903:Drug Monitoring / Q000379:methods; D006801:Humans; D012680:Sensitivity and Specificity
D019380:Anti-HIV Agents