Alkane-1,2-diol-based glycosides and fatty glycosides and wax esters in Roseiflexus castenholzii and hot spring microbial mats.
The lipid composition of Roseiflexus castenholzii, a thermophilic filamentous phototrophic bacterium related to uncultivated filamentous phototrophic bacteria that predominate in hot spring microbial mats, is reported. R. castenholzii lipid extracts were dominated by components characterized by alkane-1-ol-2-alkanoate moieties glycosidically bonded to a C(6) sugar. Similar fatty glycosides, with an additional fatty acid esterified, were detected by HPLC-MS. R. castenholzii also produces a suite of wax esters ranging from 37 to 40 carbon atoms in length. In lipid extracts from two nonsulfidic hot spring microbial mats, similar alkane-1,2-diol-based lipids were detected in minor amounts. R. castenholzii lipids are compared to lipids of mats and other thermophilic mat isolates.
Netherlands Institute for Sea Research (NIOZ), Department of Marine Biogeochemistry and Toxicology, P.O. Box 59, 1790 AB Den Burg, Texel, The Netherlands. mmeer@nioz.nl
Marcel T J
MT
van ver Meer
Stefan
S
Schouten
Satoshi
S
Hanada
Ellen C
EC
Hopmans
Jaap S Sinninghe
JS
Damsté
David M
DM
Ward
D000473:Alkanes / Q000032:analysis; D019414:Chlorobi / Q000737:chemistry*; D002849:Chromatography, Gas; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D008401:Gas Chromatography-Mass Spectrometry; D006027:Glycosides / Q000032:analysis*; D006358:Hot Temperature; D008055:Lipids / Q000032:analysis*; D014885:Waxes / Q000032:analysis
D000473:Alkanes; D004952:Esters; D005227:Fatty Acids; D006027:Glycosides; D008055:Lipids; D014885:Waxes