[Immunonutrition in experimental colitis: beneficial effects of omega-3 fatty acids].
BACKGROUND
Recent data have given emphasis to the benefits of immunonutrition with omega-3 fatty acids (n-3 FA) in various clinical situations. This work presents the results of parenteral administration of different lipid emulsions in experimental acute colitis and reviews the pertinent literature.


METHODS
Seventy-four adult male Wistar rats were randomized in six groups that had 10% acetic acid-induced colitis (except CS). During 7 days, control groups CS (without colitis) and CC (with colitis) received physiological solution and the others received specific lipid emulsion by a central venous catheter (0.5 mL/h). The n-3/n-6 FA ratio and lipidic compositions were: group L--1:7.7 (LCT, n = 12), M--1:7.0 (MCT and LCT, n = 12), LW-3--1:4.5 (LCT plus FO, n = 12) and MW-3--1:3.0 (MCT and LCT plus FO, n = 13). Rats were evaluated to assess abdominal and intestinal alterations, macrophage cellularity and colonic concentrations of LTB4, LTC4, PGE2 and TXB2.


RESULTS
N-3 FA treated rats (LW-3 and MW-3) presented less inflammatory abdominal alterations than CC rats. Mucosal ulcer formation in MW-3 group was the only comparable to CS group. Only CS, M and MW-3 rats presented smaller cellularity than CC group. Comparing to CC group, there were found smaller averages of LTB4 in CS, LW-3 and MW-3 groups, of PGE2 in CS, M and MW-3 groups, and of TXB2 in CS and MW-3 groups. LTC4 averages were not different.


CONCLUSIONS
1) LCT-containing lipid emulsion with low n-3/n-6 ratio do not modify inflammatory colitis derived manifestations; 2) the association of MCT/LCT-containing lipid emulsion with fish oil with high n-3/n-6 ratio impels great beneficial impact, attenuating morphological and inflammatory consequences and decreasing colonic concentrations of proinflammatory mediators.
Campos
Fábio Guilherme
FG
Departamento de Gastroenterologia, Faculdade de Medicina, Universidade de São Paulo, Laboratório de Metabologia e Nutrição em Cirurgia, Disciplina de Coloproctologia, Faculdade de Medicina, Universidade de São Paulo. fgmcampos@terra.com.br
Waitzberg
Dan L
DL
Logulo
Angela Flávia
AF
Torrinhas
Raquel Susana
RS
Teixeira
William Gemio Jacobsen
WG
Habr-Gama
Angelita
A
D000818:Animals; D003092:Colitis / Q000150:complications / Q000473:pathology / Q000628:therapy*; D005217:Fat Emulsions, Intravenous / Q000494:pharmacology*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000502:physiology; D008264:Macrophages; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005217:Fat Emulsions, Intravenous; D015525:Fatty Acids, Omega-3; D018836:Inflammation Mediators