Identification of negative regulator of interleukin-3 (NIL-3) in bone marrow.
We have reported that an inhibitor of interleukin-3 (NIL-3) is produced from murine bone marrow cells in response to excess stimulation of interleukin-3. In this report, we attempted the purification of the NIL-3 activity from bone marrow culture supernatant in the presence of interleukin-3. The purified NIL-3 activity was a protein with relative molecular weight of 54.5 kDa (SDS-PAGE), which inhibited the growth of IL-3 dependent DA-1 cell growth in a dose dependent manner. The N-terminal amino acid sequence of purified NIL-3 activity was determined to be homologous to beta-2 glycoprotein I (apolipoprotein H: APO-H). The gene expression of APO-H was detected by nested-PCR in STIL-3 C5-CM stimulated total bone marrow cells and STIL-3 C5-CM stimulated bone marrow fraction 2 (Fr. 2) which has been reported as a hematopoietic stem cell rich fraction. These observations indicate the possibility that the APO-H is the NIL-3 which was produced from bone marrow cells in response to excess IL-3 stimuli.
Yasuhiro
Adachi
A
Department of Cell Science, Graduate School of Science and Technology, Nigata University, Japan. adachiy@yamaguchi-u.ac.jp
Sugimoto
Kenkichi
K
Sato
Asako K
AK
Mori
Kazuhiro John
KJ
D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D005260:Female; D015870:Gene Expression / Q000276:immunology; D006023:Glycoproteins / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000378:metabolism*; D007377:Interleukin-3 / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D007938:Leukemia; D051379:Mice; D008809:Mice, Inbred C3H; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D053482:beta 2-Glycoprotein I
D006023:Glycoproteins; D007377:Interleukin-3; D012333:RNA, Messenger; D011994:Recombinant Proteins; D053482:beta 2-Glycoprotein I