Peripheral blood lymphocyte subsets in adolescents: a longitudinal analysis from the REACH project.
Flow cytometry analysis of lymphocyte subset markers was performed for a group of sexually active, human immunodeficiency virus (HIV)-negative adolescents over a 2-year period to establish normative data. Data were collected in the REACH Project (Reaching for Excellence in Adolescent Care and Health), a multicenter, longitudinal study of HIV-positive and high-risk HIV-negative adolescents. Two- and three-color flow cytometry data were collected every 6 months for these subjects. We determined the effects of gender, race, and age on the following lymphocyte subset markers: total CD4(+) cells, CD4(+) naïve cells, CD4(+) memory cells, all CD8(+) cells, CD8(+) naïve cells, CD8(+) memory cells, CD16(+) natural killer cells, and CD19(+) B cells. Gender was the demographic characteristic most frequently associated with differences in lymphocyte subset measures. Females had higher total CD4(+) cell and CD4(+) memory cells counts and lower CD16(+) cell counts than males. Age was associated with higher CD4(+) memory cell counts as well as higher CD8(+) memory cell counts. For CD19(+) cells, there was an interaction between age and gender, with males having significantly lower CD19(+) cell counts with increasing age, whereas there was no age effect for females. Race and/or ethnicity was associated with differences in total CD8(+) cell counts and CD8(+) memory cell counts, although both of these associations involved an interaction with gender.
Rudy
Bret J
BJ
Children's Hospital of Philadelphia, Department of Pediatrics, The University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, 19104, USA. rudy@email.chop.edu
Wilson
Craig M
CM
Durako
Stephen
S
Moscicki
Anna-Barbara
AB
Muenz
Larry
L
Douglas
Steven D
SD
D000293:Adolescent; D000328:Adult; D018941:Antigens, CD19 / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000166:cytology*; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000166:cytology*; D002648:Child; D015331:Cohort Studies; D005260:Female; D005434:Flow Cytometry / Q000592:standards*; D006801:Humans; D007156:Immunologic Memory; D008137:Longitudinal Studies; D008297:Male; D017452:Receptors, IgG / Q000032:analysis; D012016:Reference Values
D018941:Antigens, CD19; D017452:Receptors, IgG