Combinatorial enantiomeric separation of diverse compounds using capillary array electrophoresis.
Combinatorial chiral separations were performed on a 96-capillary array electrophoresis system. A comprehensive enantioseparation protocol employing neutral and sulfated cyclodextrins as chiral selectors for common basic, neutral and acidic compounds was developed. By using only four judiciously chosen separation buffers, successful enantioseparations were achieved for 49 out of 54 test compounds spanning a large variety of pK and structures. Therefore, unknown compounds can be screened in this manner to identify the optimal enantioselective conditions in just one run.
Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, IA, USA.
Wenwan
W
Zhong
Edward S
ES
Yeung
D002021:Buffers; D020650:Combinatorial Chemistry Techniques / Q000295:instrumentation*; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D009930:Organic Chemicals / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000302:isolation & purification; D013237:Stereoisomerism
D002021:Buffers; D003505:Cyclodextrins; D009930:Organic Chemicals; D004364:Pharmaceutical Preparations