Differences in mRNA expression, protein content, and enzyme activity of superoxide dismutases in type II pneumocytes of acute and chronic lung injury.
The lung is protected against oxidative stress by a variety of antioxidants and type II pneumocytes seem to play an important role in antioxidant defense. Previous studies have shown that inhalation of NO2 results in acute and chronic lung injury. How the expression and enzyme activity of antioxidant enzymes are influenced in type II cells of different inflammatory stages has yet not been studied. To elucidate this question, we exposed rats to 10 ppm NO2 for 3 or 20 days to induce acute or chronic lung injury. From these and air-breathing rats, type II pneumocytes were isolated. The mRNA expression and protein content of CuZnSOD and MnSOD as well as total SOD-specific enzyme activity were determined. For the acute lung injury (3 d NO2), the expression of CuZnSOD mRNA was significantly increased, while MnSOD expression was significantly reduced after 3 days of NO2 exposure. For the chronic lung injury (20 d NO2), CuZnSOD expression was still enhanced, while MnSOD expression was comparable to control. In parallel to CuZnSOD mRNA expression, the protein amount was significantly increased in acute and chronic lung injury however MnSOD protein content exhibited no intergroup differences. Total SOD enzyme activity showed a significant decrease after 3 days of NO2 exposure and was similar to control after 20 days. We conclude that during acute and chronic lung injury in type II pneumocytes expression and protein synthesis of CuZnSOD and MnSOD are regulated differently.
Division of Pneumology, Department of Internal Medicine, Philipps University of Marburg, Baldingerstrasse, Marburg D-35033, Germany. hochsche@mailer.uni-marburg.de
Renate
R
Hochscheid
Sven
S
Heinrichs
Stephan
S
Kranz
Holger
H
Garn
Bernd
B
Müller
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D017931:DNA Primers / Q000737:chemistry; D004195:Disease Models, Animal; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D009585:Nitrogen Dioxide / Q000633:toxicity; D010083:Oxidants, Photochemical; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism*
D017931:DNA Primers; D010083:Oxidants, Photochemical; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D009585:Nitrogen Dioxide