[Effect of L-arginine and N(omega)-nitro-L-arginine on oxidative phosphorylation and lipid peroxidation in rats with various tolerance to hypoxia under stressful conditions].
The influence of L-arginine (600 mg/kg) and NO-synthase blocator N omega-nitro-L-arginine L-NNA (35 mg/kg) on processes of ADP-stimulated respiration (under using 0.35 mM succinate, 1 mM alpha-ketoglutarate, 2 mM pyruvate, 2 mM glutamate, 2 Mm malate and succinate dehydrohenase blocator--2 mM malonate as substrates of oxidation), lipid peroxidation (concentration of DK and MDA), activities of succinate dehydrohenase and aminotransferases in rats tissues with different resistance to hypoxia under stress conditions have been investigated. It have been shown that the energy metabolism indices (respiration rate and efficiency of phosphorilation ADP/O) are higher in high resistent (HR) animals in the control group. Stress causes the increase of ADP-stimulated respiration in low resistent (LR) under succinate oxidation and decrease of NADPH-dependent utilization, indicative of more effort of energy system in LR animals. Stress conditions are connected with the increase of lipid peroxidation products in blood both in LR and in HR animals, though in hepar their concentration change unimportantly. Injection of L-arginine decreases aerobic component of energy metabolism on the background decreasing aminotransferases ways of oxidation and succinate dehydrohenase activity. L-arginine causes decrease of lipid peroxidation products in LR, in HR the same effect reaches by L-NNA injection. The has been made conclusion about tight correlation between energy metabolism, processes of lipid peroxidation with resistance to hypoxia and functioning of nitric oxide cycle under stress conditions.
Ikkert
O V
OV
Ivan Franko Lviv National University, Ukraine.
Kurhaliuk
N M
NM
Hordiĭ
S K
SK
D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D000860:Hypoxia; D015227:Lipid Peroxidation; D008297:Male; D009249:NADP / Q000378:metabolism; D019335:Nitroarginine / Q000494:pharmacology*; D010085:Oxidative Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism
D019335:Nitroarginine; D009249:NADP; D000244:Adenosine Diphosphate; D001120:Arginine; D013385:Succinate Dehydrogenase