Fluorescence-detected circular dichroism by modulated beam in the wavelength axial direction.
A novel device is described that permits fluorescence-detected circular dichroism (FDCD) detection in a conventional fluorescence spectrophotometer. FDCD is a phenomenon in which the emission intensity of a chiral analyte depends on the polarization state of the excitations. Although FDCD is a sensitive chiral detection technique, measurement of FDCD by using a special CD spectrometer has involved many difficulties. The proposed device consists of a polarizing prism and a retardation plate that fit on the excitation light side in the sample compartment of the fluorescence spectrophotometer. In this method, a FDCD wave superimposed on the fluorescence excitation spectrum of a chiral analyte was detected. We have also demonstrated the possibility of the enantiomeric purity determination of chiral analytes in real samples.
Toyama Institute of Health, Kosugi-machi, Japan. LEE07664@nifty.ne.jp
Atsushi
A
Yamamoto
Shuji
S
Kodama
Akinobu
A
Matsunaga
Hiroyuki
H
Nakazawa
Kazuichi
K
Hayakawa
D002392:Catechin / Q000737:chemistry*; D002942:Circular Dichroism; D004970:Estrone / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry*; D005453:Fluorescence; D013056:Spectrophotometry, Ultraviolet
D004970:Estrone; D000431:Ethanol; D002392:Catechin