Drug target discovery by gene expression analysis: cell cycle genes.
Gene expression microarrays and gene expression databases provide new opportunities for the discovery of drug targets and for determination of a drug's mode of action. We review gene expression analysis methods and describe studies that have identified cell cycle genes using differential expression analysis and co-expression analysis. We present an example of the identification of previously-unrecognized human cell cycle genes, CDCA1 through CDCA8, that are co-expressed with known cell cycle genes including CDC2, CDC7, CDC23, cyclin, MCAK, mki67a, topoisomerase II, and others.
Walker
M G
MG
Incyte Genomics and Department of Medicine, Stanford University, 1475 Flamingo Way, Sunnyvale, CA, USA. mwalker@stanfordalumni.org
D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D015195:Drug Design; D005544:Forecasting; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism
D018797:Cell Cycle Proteins; D012333:RNA, Messenger