Detection by enzyme-linked immunosorbent assay of antibodies to West Nile virus in birds.
We adapted an indirect immunoglobulin G enzyme-linked immunosorbent assay to facilitate studies of West Nile virus (WNV) and evaluated its application to taxonomically diverse avian species. Anti-WNV antibodies were detected in 23 bird species, including many exotic species, demonstrating its value in studies of WNV epizootiology.
Ebel
Gregory D
GD
The Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, Slingerlands, New York 12159, USA. ebel@wadsworth.org
Dupuis
Alan P
AP
Nicholas
David
D
Young
Donna
D
Maffei
Joseph
J
Kramer
Laura D
LD
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D001715:Bird Diseases / Q000276:immunology* / Q000821:virology*; D001717:Birds / Q000276:immunology* / Q000821:virology*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D014709:Vero Cells; D014901:West Nile Fever / Q000175:diagnosis / Q000276:immunology; D014902:West Nile virus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G