High P-glycoprotein-mediated export observed in patients with a history of idiopathic thrombocytopenic purpura.
Studies have suggested that high P-glycoprotein expression in lymphocytes from patients with autoimmune disorders may affect disease outcome. Idiopathic thrombocytopenic purpura (ITP) and Evans' syndrome are widely thought to be autoimmune processes, however, the precise mechanisms remain unknown. Peripheral blood mononuclear cells from patients with refractory or recurrent ITP or Evans' syndrome were studied using the rhodamine 123 flow cytometric assay to investigate functional export levels. Lymphocytes from ITP and Evans' syndrome patients showed a significantly decreased ability to retain rhodamine, suggesting increased export protein function. Reverse transcription polymerase chain reaction distinguished P-glycoprotein as the likely export protein.
Levy
Adam S
AS
Department of Pediatrics, New York University School of Medicine, New York, USA. Adam.Levy@med.nyu.edu
Cunningham-Rundles
Susanna
S
Mazza
BethAnne
B
Simm
Maciej
M
Gorlick
Richard
R
Bussel
James
J
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000097:blood*; D000293:Adolescent; D000328:Adult; D000368:Aged; D001327:Autoimmune Diseases / Q000097:blood*; D018929:Cell Culture Techniques; D005260:Female; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020112:Rhodamine 123 / Q000493:pharmacokinetics; D013577:Syndrome; D013921:Thrombocytopenia / Q000097:blood
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D005456:Fluorescent Dyes; D020112:Rhodamine 123