Isolation of a new member of the ecdysteroid glycoside family: 2-deoxy-20-hydroxyecdysone 22-O-beta-D-glucopyranoside.
A new ecdysteroid glycoside, 2-deoxy-20-hydroxyecdysone 22-O-beta-D-glucopyranoside, is isolated from the herb Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman. The compound is purified with multistep chromatography, such as classical column chromatography on alumina and droplet countercurrent distribution. Also, it is expanded using twice low-pressure reversed-phase liquid column chromatography. Chromatography in four steps results in the purified 2-deoxy-20-hydroxyecdysone 22-O-beta-D-glucopyranoside. Two other ecdysteroids have also been separated, including the formerly identified integristerone A and 24(28)-dehydromakisterone A.
Báthori
Mária
M
Department of Pharmacognosy, University of Szeged, Hungary. bathori@pharma.szote.u-szeged.hu
Pongrácz
Zita
Z
Tóth
Gábor
G
Simon
András
A
Kandra
Lili
L
Kele
Zoltán
Z
Ohmacht
Róbert
R
D029748:Caryophyllaceae / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D026461:Ecdysteroids / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D013058:Mass Spectrometry; D009005:Monosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification*
C465597:2-deoxy-20-hydroxyecdysone-22-O-glucopyranoside; D026461:Ecdysteroids; D006027:Glycosides; D009005:Monosaccharides; D013256:Steroids