Amine fluoride gel affects the viability and the generation of superoxide anions in human polymorphonuclear leukocytes: an in vitro study.
Amine hydrofluorides are widely used to prevent caries. As an acidulated gel, they were also studied for their applicability to reduce pathogenic bacteria in periodontal pockets. We assessed the toxicity of this pharmaceutical amine hydrofluoride preparation on human polymorphonuclear leukocytes in vitro by measuring Trypan blue exclusion and the generation of superoxide anions (O2) by the chemotactic peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP) after a 3-min contact with gel. Depending on the experimental conditions, gel dilutions up to 1.3 x 10(4) resulted in an increase in Trypan blue-colored cells and liberation of beta-glucuronidase. Dilutions between 3 x 10(4) and 1 x 10(5) augmented the fMLP-mediated O2- generation, which could be prevented by Ca2+ chelation with BAPTA-AM (1,2'-bis (o-aminophenoxyethane-N.N.N'.N'-tetraacetic acid tetra (acetoxymethyl) ester) and ethyleneglycoltetraacetic acid (EGTA) or inhibition of protein kinase C (PKC) with staurosporine and bisindolylmaleimide I. respectively. Compared with data published on the minimal inhibitory concentration for periodontal pathogenic bacteria, the cytotoxicity of amine hydrofluorides on eukaryotic cells is much greater and thus of consequence for their clinical use.
Institute of Pharmacology, Benjamin Franklin Medical Center, Freie Universität Berlin, Germany. kno@zedat.fu-berlin.de
Elisabeth
E
Knoll-Köhler
Juliane
J
Stiebel
D000588:Amines / Q000633:toxicity*; D002327:Cariostatic Agents / Q000633:toxicity*; D015550:Cell Degranulation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003959:Diamines; D005459:Fluorides / Q000633:toxicity; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D013481:Superoxides / Q000378:metabolism*; D014002:Tin Fluorides / Q000633:toxicity*
D000588:Amines; D002327:Cariostatic Agents; D003959:Diamines; D014002:Tin Fluorides; D013481:Superoxides; C075174:amine fluoride gel; D009240:N-Formylmethionine Leucyl-Phenylalanine; C495399:Olaflur; D005459:Fluorides