Preparative isolation of polyphenolic compounds from Vitis vinifera by centrifugal partition chromatography.
This study deals with a centrifugal partition chromatography developed for the separation of phenolic compounds from Vitis vinifera. EtOAc grape seed extracts were separated using the solvent system hexane-ethyl acetate-ethanol-water (1:8:2:7; v/v) in two fractions: one containing about 75% of flavanol monomers (catechin and epicatechin) corresponding to 18% of crude extract and another fraction B-type dimers (22% of crude extract). From the stalk extracts, we could separate stilbenoid compounds (resveratrol and its oligomers; 12% of crude extract) which were eluted in less than 30 min from flavanols (which required a few hours of additional elution). Using the same solvent system but in different ratios (4:5:3:3; v/v), we isolated the trans-resveratrol (7@1000; 90% purity).
Laboratoire de Pharmacognosie, EA no. 491, Faculté des Sciences Pharmaceutiques, Université Victor Segalen Bordeaux 2, 146 Rue Léo-Saignat, 33076 Bordeaux, France.
Jean-Claude
JC
Delaunay
Chantal
C
Castagnino
Catherine
C
Chèze
Joseph
J
Vercauteren
D002498:Centrifugation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids; D010636:Phenols / Q000302:isolation & purification*; D011108:Polymers / Q000302:isolation & purification*; D059808:Polyphenols; D013056:Spectrophotometry, Ultraviolet; D027843:Vitis / Q000737:chemistry*
D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D059808:Polyphenols