Determination of clindamycin in animal plasma by high-performance liquid chromatography combined with electrospray ionization mass spectrometry.
A method for the quantification of clindamycin in animal plasma using high-performance liquid chromatography combined with electrospray ionization mass spectrometry (LC/ESI-MS/MS) is presented. Lincomycin is used as the internal standard. The sample preparation includes a simple deproteinization step with trichloroacetic acid. Chromatographic separation is achieved on an RP-18 Hypersil column using gradient elution with 0.01 M ammonium acetate and acetonitrile as mobile phase. Good linearity was observed in the range 0-10 microg ml(-1). The limit of quantification of the method is 50 ng ml(-1) and the limit of detection is 1.3 ng ml(-1). The method was shown out to be of use for pharmacokinetic studies of clindamycin formulations in dogs.
Faculty of Veterinary Medicine, Department of Pharmacology, Pharmacy and Toxicology, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. mcherlet@yahoo.com
Marc
M
Cherlet
Siska
S
Croubels
Patrick
P
De Backer
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002981:Clindamycin / Q000097:blood*; D004285:Dogs; D008034:Lincomycin / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
D000900:Anti-Bacterial Agents; D002981:Clindamycin; D008034:Lincomycin