Envelope sequence variation and phylogenetic relations of human T cell lymphotropic virus type 1 from endemic areas of Colombia.
The HTLV-1 envelope gene of 12 TSP/HAM patients from two endemic areas of southwest Colombia (Tumaco and Buenaventura) was amplified by nested PCR, sequenced, and compared with previously reported HTLV-1 envelope sequences from isolates worldwide. In general, the sequence divergences among all Colombian samples ranged from 0.1 to 1.6%. Some amino acid substitutions, referring to the ATK-1 prototype strain in the surface domain gp46 and in p21, were highly prevalent in southwest Colombia, suggesting a geographical clustering of mutations in the envelope gene. The phylogenetic analysis showed that the Colombian isolates belong to the HTLV-1a lineage with minor subgroups. The genetic distance between Colombian and Japanese isolates ranged from 0.1 to 1.8%; in comparison, the genetic distance between Colombian and Caribbean isolates ranged from 0.4 to 2.2%. Our results strongly suggest that the actual quasispecies populations in southwest Colombia have been generated by separate, differently timed introductions of virus.
Dominguez
Martha C
MC
Laboratory of Molecular Biology and Pathogenesis, Department of Physiological Sciences, Faculty of Health, University of Valle, 25360 Cali, Colombia.
Castillo
Andres
A
Cabrera
Jesus
J
Eizuru
Yoshito
Y
Garcia-Vallejo
Felipe
F
D001483:Base Sequence; D003105:Colombia; D017931:DNA Primers; D015752:Genes, env; D015368:Human T-lymphotropic virus 1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D015493:Paraparesis, Tropical Spastic / Q000821:virology*; D010802:Phylogeny; D017354:Point Mutation
D017931:DNA Primers