Effect of aging on gene expression rates of endothelin-1 and endothelin-2/vasoactive intestinal contractor in ethanol-induced gastric mucosal injury of the mouse.
To elucidate the physiological roles of endothelin-1 (ET-1) and endothelin-2 (ET-2)/vasoactive intestinal contractor (VIC) in gastric injury of mice, we measured the gene expression rates of ET-1 and ET-2/VIC in gastric injury induced by ethanol in young (8 weeks) and old (>33 weeks) mice. Mice that were fasted for 24 h were injected with absolute ethanol intragastrically and killed after 1 or 4 h of ethanol exposure. The size of the gastric lesions increased gradually after ethanol exposure and was at its greatest after 4 h, in both young and old mice. The gene expression of ET-1 tended to increase after 1 h and to decrease by 4 h of ethanol exposure in both young and old mice. However, the gene expression of ET-2/VIC in young mice increased significantly after 1 and 4 h of ethanol exposure, whereas the gene expression of ET-2/VIC in the old mice did not change after ethanol exposure. Based on these results, we conclude that aging influences the gene expression of ET-2/VIC but not lesion size or gene expression of ET-1 in ethanol-induced gastric injury in the mouse. We therefore suggest that regulation of gene expression of these two genes differs during the course of aging.
Institute of Molecular and Cell Biology, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8566, Japan.
Takaharu
T
Kozakai
Hong
H
Zhao
Mitsue
M
Sakate
Yoshinori
Y
Masuo
Tsuyoshi
T
Uchide
Kaname
K
Saida
D000375:Aging / Q000502:physiology*; D000818:Animals; D019332:Endothelin-1 / Q000235:genetics*; D019333:Endothelin-2 / Q000235:genetics*; D000431:Ethanol; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D023421:Models, Animal; D018919:Neovascularization, Physiologic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013276:Stomach Ulcer / Q000378:metabolism*
D019332:Endothelin-1; D019333:Endothelin-2; D000431:Ethanol