Optical barcoding of colloidal suspensions: applications in genomics, proteomics and drug discovery.
The enormous amount of information generated through sequencing of the human genome has increased demands for more economical and flexible alternatives in genomics, proteomics and drug discovery. Many companies and institutions have recognised the potential of increasing the size and complexity of chemical libraries by producing large chemical libraries on colloidal support beads. Since colloid-based compounds in a suspension are randomly located, an encoding system such as optical barcoding is required to permit rapid elucidation of the compound structures. We describe in this article innovative methods for optical barcoding of colloids for use as support beads in both combinatorial and non-combinatorial libraries. We focus in particular on the difficult problem of barcoding extremely large libraries, which if solved, will transform the manner in which genomics, proteomics and drug discovery research is currently performed.
Centre for Nanotechnology and Biomaterials, Department of Chemistry, University of Queensland, St. Lucia, QLD 4072, Australia.
Bronwyn J
BJ
Battersby
Gwendolyn A
GA
Lawrie
Angus P R
AP
Johnston
Matt
M
Trau
D003102:Colloids / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques; D001330:Electronic Data Processing; D015698:Genomic Library; D023281:Genomics / Q000379:methods*; D006801:Humans; D010600:Pharmacology / Q000379:methods*; D020543:Proteome / Q000737:chemistry*; D013535:Suspensions
D003102:Colloids; D020543:Proteome; D013535:Suspensions