[The role of cell proliferation in tubulogenesis of human keratinocytes].
We studied kinetics and role of keratinocyte proliferation in the process of cysto- and tubulogenesis of human postnatal epidermal keratinocytes in collagen gel. The influence of media conditioned by embryonic and postnatal fibroblasts on morphogenesis has been comparatively analyzed. We studied the influence of proliferation inhibitors and inductors on tubulogenesis. The obtained data indicate partial dissociation of migration, proliferation, and differentiation of keratinocytes during morphogenetic processes in culture. We propose a new model considering proliferation of epithelial cells during cysto- and tubulogenesis.
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 117808 Russia. vsh@grey.ru
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Chernaia
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D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology; D005782:Gels; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D008870:Microtubules / Q000187:drug effects / Q000502:physiology*; D016685:Mitomycin / Q000494:pharmacology; D051381:Rats; D013936:Thymidine / Q000494:pharmacology
D005782:Gels; D016685:Mitomycin; D004815:Epidermal Growth Factor; D003094:Collagen; D013936:Thymidine