Autogenous demineralized dentin matrix for tissue engineering applications: radiographic and histomorphometric studies.
OBJECTIVE
This work evaluated the osteoconductive properties of autogenous demineralized dentin matrix (ADDM) on surgical bone defects in the parietal bone of rabbits, using the guided bone regeneration technique and polytetrafluoroethylene (PTFE) membrane.


METHODS
Surgical bone defects were created in 24 adult rabbits and repaired with either ADDM and PTFE (experimental group) or PTFE alone (control group). The ADDM had been obtained from the central incisors of the experimental rabbits. The rabbits were sacrificed after 15, 30, 60, and 90 days and the defects examined radiographically and histologically.


RESULTS
Radiographically, the defects in the experimental animals achieved radiopacity more quickly than the defects in the control group.


CONCLUSIONS
After 15, 30, 60, and 90 days of observation following surgery, the ADDM slices appeared to stimulate new bone formation. The dentin slices were completely incorporated into the new bone tissue and were resorbed during the bone repair.


CONCLUSIONS
Bone repair was accelerated on the bone defects treated with ADDM when compared to the control group.
Gomes
Monica Fernandes
MF
Department of Biosciences and Oral Diagnosis, Dental School, São Paulo State University, UNESP, São Paulo, Brazil. mfgomes@fosjc.unesp.br
dos Anjos
Mário James da Silva
MJ
Nogueira
Terezinha de Oliveira
Tde O
Catanzaro Guimarães
Sérgio Augusto
SA
D000818:Animals; D015519:Bone Density; D001861:Bone Regeneration; D003648:Decalcification Technique; D003764:Dental Materials; D003804:Dentin; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D008567:Membranes, Artificial; D010294:Parietal Bone / Q000033:anatomy & histology / Q000000981:diagnostic imaging / Q000601:surgery; D011138:Polytetrafluoroethylene; D011817:Rabbits; D011859:Radiography; D023822:Tissue Engineering / Q000379:methods*
C093073:Demineralized Dentin Matrix; D003764:Dental Materials; D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene