Gene expression changes during mouse skeletal myoblast differentiation revealed by transcriptional profiling.
Studies described here utilize high-density oligonucleotide arrays to characterize changes in global mRNA expression patterns during proliferation, cell cycle withdrawal, and terminal differentiation in mouse C2C12 myoblasts. Statistical analyses revealed 629 sequences differentially regulated between proliferating and differentiating myoblasts. These genes were clustered using self-organizing maps to identify sets of coregulated genes and were assigned to functional categories that were analyzed for distribution across expression clusters. Clusters were identified with statistically significant enrichment of functional categories including muscle contraction, cell adhesion, extracellular matrix function, cellular metabolism, mitochondrial transport, DNA replication, cell cycle control, mRNA transcription, and unexpectedly, immune regulation. In addition, functional category enrichment data can be used to predict gene function for numerous differentially regulated expressed sequence tags. The results provide new insight into how genes involved in these cellular processes may play a role in skeletal muscle growth and differentiation.
Department of Genomics, Wyeth Research, Cambridge, Massachusetts 02140, USA.
Jennifer L
JL
Moran
Yizheng
Y
Li
Andrew A
AA
Hill
William M
WM
Mounts
Christopher P
CP
Miller
D000818:Animals; D002448:Cell Adhesion / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D004261:DNA Replication; D005109:Extracellular Matrix / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D007109:Immunity / Q000235:genetics; D051379:Mice; D005810:Multigene Family; D009119:Muscle Contraction / Q000235:genetics; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger; D014158:Transcription, Genetic
D002843:Chromatin; D012333:RNA, Messenger