[Determination of sarsasapogenin in Anemarrhena asphodeloides Bunge by GC].
OBJECTIVE
To determine sarsasapogenin in Anemarrhena asphodeloides.


METHODS
Chloromethane extract (1 microliter) with cholesterol as internal standard was analyzed on HP-1 column, operated at 270 degrees C with N2 as carrier gas and FID.


RESULTS
The method was linear within the range of 0.245-2.94 micrograms.microliter-1, with a correlation coefficient of 0.9996. The average recovery was 95.52% +/- 1.77%,


CONCLUSIONS
The method is reproducible, rapid and sensitive.
Department of Education Guarantee, Second Military Medical University, Shanghai 200433.
Y
Y
Liu
W
W
Chen
C
C
Qiao
N
N
Zhao
D031403:Anemarrhena / Q000737:chemistry*; D002849:Chromatography, Gas; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D013150:Spirostans / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D013150:Spirostans; C046297:sarsasapogenin