[Construction and expression of recombinant retrovirus vector carrying HBV vector].
BACKGROUND
To explore the possibility of using retrovirus vector to carry HBV vector, and to prove that replication defective HBV could be normally packaged.


METHODS
Two kinds of full length of mutant HBV gene, which express dominant negative mutants, were inserted into retrovirus vector. After recombinant retroviruses were harvested, they were used to infect Hep G2 and 2.2.15 cell line. Then the expression of HBV core antigen in the Hep G2 cell was examined by immune fluorescence, and the existence of recombinant HB virion in the culture medium was examined by PCR.


RESULTS
High titer of recombinant retroviruses were obtained in the culture medium of transfected PA317 cell line. Core antigen was detectable in the recombinant retrovirus infected Hep G2 cell. Recombinant HB virion was detectable in the culture medium of recombinant retrovirus infected 2.2.15 cell.


CONCLUSIONS
The results suggested that recombinant retrovirus could carry HBV vector and express HBV products. When structural protein is offered by wt-HBV, the recombinant retrovirus may function as HBV vector, therefore it could be used in anti?HBV gene therapy.
Institute of Hepatology, Beijing Army General Hospital, Beijing 100700, China.
Dianxing
D
Sun
Darong
D
Hu
Guanghui
G
Wu
Xueling
X
Hu
Juan
J
Li
Gongren
G
Fan
D015316:Genetic Therapy; D005822:Genetic Vectors; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D006512:Hepatitis B Core Antigens