RNA chaperone StpA loosens interactions of the tertiary structure in the td group I intron in vivo.
Efficient splicing of the td group I intron in vivo is dependent on the ribosome. In the absence of translation, the pre-mRNA is trapped in nonnative-splicing-incompetent conformations. Alternatively, folding of the pre-mRNA can be promoted by the RNA chaperone StpA or by the group I intron-specific splicing factor Cyt-18. To understand the mechanism of action of RNA chaperones, we probed the impact of StpA on the structure of the td intron in vivo. Our data suggest that StpA loosens tertiary interactions. The most prominent structural change was the opening of the base triples, which are involved in the correct orientation of the two major intron core domains. In line with the destabilizing activity of StpA, splicing of mutant introns with a reduced structural stability is sensitive to StpA. In contrast, Cyt-18 strengthens tertiary contacts, thereby rescuing splicing of structurally compromised td mutants in vivo. Our data provide direct evidence for protein-induced conformational changes within catalytic RNA in vivo. Whereas StpA resolves tertiary contacts enabling the RNA to refold, Cyt-18 contributes to the overall compactness of the td intron in vivo.
Institute of Microbiology and Genetics, University of Vienna, Vienna Biocenter, A-1030 Vienna, Austria.
Christina
C
Waldsich
Rupert
R
Grossberger
Renée
R
Schroeder
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D017122:Bacteriophage T4 / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D018389:Codon, Nonsense; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D007438:Introns; D018832:Molecular Chaperones / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009492:Neurospora crassa / Q000201:enzymology; D009690:Nucleic Acid Conformation; D012326:RNA Splicing; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D013940:Thymidylate Synthase / Q000235:genetics*
D018389:Codon, Nonsense; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D012367:RNA, Viral; C090104:StpA protein, E coli; D013940:Thymidylate Synthase; D000604:Amino Acyl-tRNA Synthetases