Simple and reliable method for detection and genotyping of hepatitis C virus RNA in dried blood spots stored at room temperature.
We describe a simple, sensitive, and reproducible method for using whole blood collected onto filter paper (dried blood spots) for detection and genotyping of hepatitis C virus RNA that can be useful in large field studies, particularly in settings where collection, preparation, storage, and shipment of samples at controlled temperature can be difficult.
National Institute for Infectious Diseases L. Spallanzani, IRCCS, Rome, Italy.
Mariacarmela
M
Solmone
Enrico
E
Girardi
Francesco
F
Costa
Leopoldo
L
Pucillo
Giuseppe
G
Ippolito
Maria R
MR
Capobianchi
D001800:Blood Specimen Collection / Q000379:methods*; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000821:virology; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D015203:Reproducibility of Results; D013696:Temperature
D012367:RNA, Viral