Evaluation of inhouse rapid urease test for detection of Helicobacter pylori from gastric biopsy specimens.
Inhouse rapid urease test for detecting Helicobacter pylori was evaluated. Biopsy specimens were taken for inhouse urease test, commercial rapid urease CLO test, culture, gram stain and histology from the antrium or duodenum of patients who had peptic ulcer. The culture and/or histologic examination and CLO test were used as the gold standard. One hundred and twelve specimens were evaluated. The sensitivity and specificity of the inhouse urease test was 100 per cent and 90 per cent respectively. The inhouse urease test was suitable for detecting Helicobacter pylori from gastric antral biopsy specimens. The medium can be kept in a refrigerator for up to 6 months.
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Pongpun
P
Nunthapisud
Kanchalee
K
Lertpocasombat
Orrawadee
O
Hanvivatvong
Kamjorn
K
Tatiyakavee
Duangporn
D
Thong-Ngam
Pinit
P
Kullavanijaya
Sutep
S
Gonlachanvit
Sombat
S
Treeprasertsuk
Pongsapeera
P
Suwanagool
D001707:Biopsy, Needle; D015169:Colony Count, Microbial; D003470:Culture Media; D046508:Culture Techniques; D005753:Gastric Mucosa / Q000382:microbiology* / Q000473:pathology; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D007297:Inpatients; D012680:Sensitivity and Specificity; D013276:Stomach Ulcer / Q000382:microbiology / Q000473:pathology; D014510:Urease / Q000032:analysis*
D003470:Culture Media; D014510:Urease