Determination of sulfonamide residues in eggs by liquid chromatography.
A method was developed for determining residual sulfonamide antibacterials such as sulfamethazine (SMZ), sulfamonomethoxine (SMM), sulfadimethoxine (SDM), and sulfaquinoxaline (SQ) in eggs using liquid chromatography with a photodiode array detector. The spiked and blank samples were cleaned up by using an Ultrafree-MC/PL centrifugal ultrafiltration unit. A Mightysil RP-4 GP column and a mobile phase of 28% (v/v) ethanol-H2O with a photodiode array detector were used for the determination. Average recoveries from eggs spiked with each drug at 0.1, 0.2, 0.4, and 1.0 ppm were > or = 80.9%, with relative standard deviations between 1.3 and 4.7%. The limits of quantitation were 0.060 ppm for SMZ, 0.045 for SMM, 0.044 for SDM, and 0.093 for SQ. The analysis of one sample required < 30 min and < 5 mL ethanol as solvent.
Furusawa
Naoto
N
Osaka City University, Faculty of Human Life Science, Osaka, Japan. furusawa@life.osaka-cu.ac.jp
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D012997:Solvents; D013412:Sulfadimethoxine / Q000032:analysis; D013418:Sulfamethazine / Q000032:analysis; D013422:Sulfamonomethoxine / Q000032:analysis; D013428:Sulfaquinoxaline / Q000032:analysis; D013449:Sulfonamides / Q000032:analysis*
D000890:Anti-Infective Agents; D012997:Solvents; D013449:Sulfonamides; D013412:Sulfadimethoxine; D013418:Sulfamethazine; D013422:Sulfamonomethoxine; D013428:Sulfaquinoxaline