Effect of cyclosporin A treatment on renal calcium oxalate binding in experimental hyperoxaluria.
This study was aimed to investigate the effect ofCyclosporin A administration on renal calcium oxalate binding under hyperoxaluric condition. Cyclosporin A administration or ammonium oxalate treatment increased calcium oxalate binding, which was further increased in kidney treated with cyclosporin A and ammonium oxalate together. The increase of calcium oxalate binding was associated with lipid peroxidation as well as with a concomitant decrease in total thiol in both rat and human kdiney homogenate. Cyclosporin A administration to hyperoxaluric rats resulted with increased calcium oxalate binding protein. However there was no change with specific activity of the protein. In conclusion, Cyclosporin A administration either to normal or hyperoxaluric rats is resulted with increased concentration of calcium oxalate binding protein as well as enhanced activity due to membrane lipid peroxidation.
Adhirai
Marimuthu
M
Department of Medical Biochemistry, Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, India.
Sakthivel
Ramasamy
R
Selvam
Ramasamy
R
D000818:Animals; D002129:Calcium Oxalate / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D016572:Cyclosporine / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D010070:Oxalates / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D013997:Time Factors
D004791:Enzyme Inhibitors; D010070:Oxalates; D002129:Calcium Oxalate; D016572:Cyclosporine