Gene expression profiling of the aging mouse cardiac myocytes.
Heart disease remains the most frequent cause of death in the general population with increasing incidence in the elderly population. The pathologic failure of the aging heart may be related to structural and functional alterations in cardiac muscle cells. However, the molecular mechanisms underlying the aging-related decline in cardiac muscle function are largely unknown. To provide the first analysis of cardiac aging at the level of gene expression, we established and compared cDNA libraries from apparently healthy young and aged mouse ventricular cardiac muscle cells. We report the identification of genes that exhibit aging-related changes of mRNA levels. Aging expression profiles in aged hearts indicate decreased cellular adaptation and protection against stress-induced injury together with the development of contractile dysfunction. The data suggest reduced activity of the mitochondrial electron transport system and reduced levels of cardiac-specific transcription regulators. The cardiomyocyte aging profile of gene expression displays similarities with known heart disorders. Genes whose mRNA levels change with aging in cardiomyocytes might profoundly affect pathological changes in the heart.
Division of Endocrinology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Natalya
N
Bodyak
Peter M
PM
Kang
Makoto
M
Hiromura
Indra
I
Sulijoadikusumo
Nobuo
N
Horikoshi
Konstantin
K
Khrapko
Anny
A
Usheva
D000185:Actinin / Q000737:chemistry; D000375:Aging / Q000502:physiology*; D000818:Animals; D015152:Blotting, Northern; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D020869:Gene Expression Profiling; D015723:Gene Library; D006352:Heart Ventricles / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D012333:RNA, Messenger; D000185:Actinin; D012313:RNA; D016650:Fluorescein-5-isothiocyanate