Schemes of flux control in a model of Saccharomyces cerevisiae glycolysis.
We used parameter scanning to emulate changes to the limiting rate for steps in a fitted model of glucose-derepressed yeast glycolysis. Three flux-control regimes were observed, two of which were under the dominant control of hexose transport, in accordance with various experimental studies and other model predictions. A third control regime in which phosphofructokinase exerted dominant glycolytic flux control was also found, but it appeared to be physiologically unreachable by this model, and all realistically obtainable flux control regimes featured hexose transport as a step involving high flux control.
Institute of Biological Sciences, University of Wales, Aberystwyth, UK.
Leighton
L
Pritchard
Douglas B
DB
Kell
D000255:Adenosine Triphosphate / Q000378:metabolism; D000465:Algorithms; D001692:Biological Transport; D003198:Computer Simulation; D005947:Glucose / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism; D006019:Glycolysis; D007700:Kinetics; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism
D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D006003:Glycogen; D014199:Trehalose; D005947:Glucose