Steroids in Duchenne muscular dystrophy: from clinical trials to genomic research.
Steroids represent the only pharmacological palliative treatment for Duchenne muscular dystrophy. However, they do have side effects and despite a large number of published studies showing their efficacy, they are still not universally used. This is largely due to the lack of functional outcome and quality of life measures in most of the published studies and suggests that further trials might be required to answer some of the still unclear aspects of their role. Another important aspect of steroid therapy in Duchenne dystrophy is that we do not know how they work in dystrophic muscle. We have initiated a collaborative study on gene profiling using microarray in steroid-treated mdx mice. cDNA microarray studies were performed to examine the levels of skeletal muscle gene expression in a pool of mdx mice treated with prednisolone for 1 and 6 weeks. Interesting preliminary data on untreated mdx mice suggest that the gene profiling of young (7 weeks) versus older (12 weeks) mice is very significantly different. Furthermore, a large number of genes showed significant changes in expression at the mRNA level on treatment with prednisolone. These included structural protein genes; signalling genes and genes involved in immune response. Hopefully, analysis of this pattern of steroid-induced gene expression will provide some insight into understanding how glucocorticoids improve strength in Duchenne dystrophy, and may help in developing more effective and less toxic therapeutic approaches.
Muntoni
Francesco
F
The Dubowitz Neuromuscular Centre, Hammersmith Hospital, Du Cane Road, London W12 ONN, UK. f.muntoni@ic.ac.uk
Fisher
Ivan
I
Morgan
Jennifer E
JE
Abraham
David
D
D000305:Adrenal Cortex Hormones / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D020869:Gene Expression Profiling; D005938:Glucocorticoids / Q000494:pharmacology*; D051379:Mice; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism*; D009137:Muscular Dystrophy, Animal / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D020388:Muscular Dystrophy, Duchenne / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011239:Prednisolone / Q000494:pharmacology*; D012333:RNA, Messenger
D000305:Adrenal Cortex Hormones; D005938:Glucocorticoids; D012333:RNA, Messenger; D011239:Prednisolone