Detection of viral infection and gene expression in clinical tissue specimens using branched DNA (bDNA) in situ hybridization.
In situ hybridization (ISH) methods for detection of nucleic acid sequences have proved especially powerful for revealing genetic markers and gene expression in a morphological context. Although target and signal amplification technologies have enabled researchers to detect relatively low-abundance molecules in cell extracts, the sensitive detection of nucleic acid sequences in tissue specimens has proved more challenging. We recently reported the development of a branched DNA (bDNA) ISH method for detection of DNA and mRNA in whole cells. Based on bDNA signal amplification technology, bDNA ISH is highly sensitive and can detect one or two copies of DNA per cell. In this study we evaluated bDNA ISH for detection of nucleic acid sequences in tissue specimens. Using normal and human papillomavirus (HPV)-infected cervical biopsy specimens, we explored the cell type-specific distribution of HPV DNA and mRNA by bDNA ISH. We found that bDNA ISH allowed rapid, sensitive detection of nucleic acids with high specificity while preserving tissue morphology. As an adjunct to conventional histopathology, bDNA ISH may improve diagnostic accuracy and prognosis for viral and neoplastic diseases.
Kenny
Daryn
D
Bayer Diagnostics, Berkeley, California 94702-0466, USA.
Shen
Lu-Ping
LP
Kolberg
Janice A
JA
D015415:Biomarkers; D021121:Branched DNA Signal Amplification Assay; D002584:Cervix Uteri / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005838:Genotype; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D015345:Oligonucleotide Probes; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000821:virology*
D015415:Biomarkers; D004279:DNA, Viral; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012367:RNA, Viral