Effect of periodontal treatment on the activity of chitinase in whole saliva of periodontitis patients.
Human salivary chitinase could play a role in the defence against chitin-containing oral pathogens. The activity levels of chitinase in the whole saliva of periodontitis patients were significantly higher than those in saliva from controls. Periodontal treatment for a period of 5-6 months resulted in a three- to fourfold decrease in this enzyme activity. The activity of beta-N-acetylhexosaminidase, which is another enzyme that hydrolyses glycosidic linkages, also decreased as a result of treatment, although to a lesser extent. The decrease in chitinase activity upon treatment of the disease did not correlate with the decrease that was seen in clinical attachment loss and bleeding on probing, and only a weak correlation was observed with the changes in probing pocket depth and plaque index. No correlations were found between the above clinical parameters and the decrease in beta-N-acetylhexosaminidase activity.
Department of Medical Chemistry, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
G J
GJ
Van Steijn
A V Nieuw
AV
Amerongen
E C I
EC
Veerman
S
S
Kasanmoentalib
B
B
Overdijk
D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D002688:Chitinases / Q000032:analysis*; D003774:Dental Plaque Index; D012534:Dental Scaling; D005260:Female; D005500:Follow-Up Studies; D005884:Gingival Hemorrhage / Q000201:enzymology / Q000628:therapy; D006801:Humans; D008297:Male; D008875:Middle Aged; D009910:Oral Hygiene; D017622:Periodontal Attachment Loss / Q000201:enzymology / Q000628:therapy; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000201:enzymology / Q000628:therapy; D010518:Periodontitis / Q000201:enzymology / Q000628:therapy*; D012044:Regression Analysis; D016745:Root Planing; D012463:Saliva / Q000201:enzymology*; D012471:Salivary Proteins and Peptides / Q000032:analysis; D013223:Statistics as Topic; D013357:Subgingival Curettage; D001619:beta-N-Acetylhexosaminidases / Q000032:analysis
D012471:Salivary Proteins and Peptides; D002688:Chitinases; D001619:beta-N-Acetylhexosaminidases