Syntheses of polycationic dendrimers on lipophilic peptide core for complexation and transport of oligonucleotides.
Synthesis of novel polycationic lipophilic peptide core(s) was accomplished and these agents successfully transfected human retinal pigment epithelium cells with ODN1 upon complexation with the oligonucleotide. The level of transfection was indirectly measured by the decreased production of the protein hVEGF (human vascular endothelial growth factor) in comparison to the transfection agent cytofectin GSV.
Wimmer
Norbert
N
School of Pharmacy, The University of Queensland, Steele Building, St. Lucia, Qld 4072, Australia.
Marano
Robert J
RJ
Kearns
Philip S
PS
Rakoczy
Elizabeth P
EP
Toth
Istvan
I
D002478:Cells, Cultured; D016228:Endothelial Growth Factors / Q000235:genetics; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D008222:Lymphokines / Q000235:genetics; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D010455:Peptides / Q000737:chemistry*; D011073:Polyamines / Q000138:chemical synthesis* / Q000737:chemistry; D000071228:Polyelectrolytes; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D009841:Oligonucleotides; D010455:Peptides; D011073:Polyamines; D000071228:Polyelectrolytes; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors