Detection of increased icb-1 transcript levels in maturing HL-60 cells: a novel marker for granulocytic and monocytic in vitro differentiation.
Human gene icb-1 was initially described as a gene with increased expression in endometrial tumor cells differentiated in vitro by culture on a reconstituted basement membrane. We provide evidence for a more general involvement of icb-1 gene function in cellular differentiation processes. We report the up-regulation of icb-1 transcript levels in HL-60 promyelocytic leukemia cells during their in vitro differentiation induced by all-trans retinoic acid, vitamin D(3) or DMSO. Increased icb-1 mRNA levels could be observed both in monocytic and granulocytic differentiation. We also report the identification of the novel icb-1 splice variants icb-1beta and gamma, and the spleen-specific isoform icb-1delta. Expression of icb-1 can be used as a novel marker for in vitro differentiation processes of HL-60 cells.
Treeck
Oliver
O
Department of Obstetrics and Gynecology, Medical University of Lubeck, Ratzeburger Allee 160, D-23538 Lubeck, Germany. treeck@email.com
Odani
Tomoyuki
T
Itoh
Norio
N
Imai
Harue
H
Fujita
Sayaka
S
Kohroki
Jyunya
J
Nakanishi
Tsuyoshi
T
Diedrich
Klaus
K
Ortmann
Olaf
O
Tanaka
Keiichi
K
Vollmer
Günter
G
D017398:Alternative Splicing; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation / Q000235:genetics; D020869:Gene Expression Profiling; D006098:Granulocytes / Q000166:cytology; D018922:HL-60 Cells / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D009000:Monocytes / Q000166:cytology; D009363:Neoplasm Proteins / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism*; D015533:Transcriptional Activation; D015854:Up-Regulation
D015415:Biomarkers; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C111850:THEMIS2 protein, human