Testing biomaterials by the in-situ evaluation of cell response.
In this work, we describe a technique for the in-situ observation of cells adhered on opaque biomaterial surfaces. The visualisation of the morphology of cells adhered onto a surface allows to derive nuclear apoptotic signs or even the existence of organisation between groups of these cells. The technique is based on the use of an auto-immune reaction combined with a fluorescent agent that allows a direct inspection of the cell behaviour. The versatility of the technique is demonstrated by presenting several examples with different cultured cells (human chondrosarcome cells (CSRCs) and pluripotential mesenchymal stem cells (MSCs) from bone marrow) seeded over two different Ti-based surfaces (TiO(2) and TiN, respectively). These in-vitro observations are compared with the behaviour of the same cells on bare TiAlV alloy. From our results it is concluded that both TiO(2) and TiN surfaces show enhanced biological responses.
Dpto. Física Aplicada C-XII, Universidad Autónoma de Madrid, 28049 Madrid, Spain. miguel.manso@uam.es
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M
Manso
S
S
Ogueta
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Pérez-Rigueiro
J P
JP
García
J M
JM
Martínez-Duart
D000497:Alloys; D002448:Cell Adhesion / Q000276:immunology; D002455:Cell Division; D002478:Cells, Cultured; D002813:Chondrosarcoma / Q000276:immunology* / Q000473:pathology*; D020099:Coated Materials, Biocompatible; D003599:Cytoskeleton; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D008422:Materials Testing / Q000379:methods*; D008648:Mesoderm / Q000276:immunology; D014025:Titanium
D000497:Alloys; D020099:Coated Materials, Biocompatible; C031462:titanium alloy (TiAl6V4); C009495:titanium dioxide; C041500:titanium nitride; D014025:Titanium