Gender differences in contractile and relaxing responses of aortic rings from lipopolysaccharide-treated rats.
Aortic rings from male and female Sprague-Dawley rats were placed in organ chambers filled with Krebs solution, and the isometric tension was recorded. There were no gender differences in responses to potassium chloride or phenylephrine in either intact or endothelium-denuded rings from control animals. Treatment with 10 mg/kg of lipopolysaccharide (LPS) attenuated the contractile response to potassium chloride in intact, but not in endothelium-denuded rings, and suppressed the phenylephrine contractions in rings both with and without endothelium from male and female rats. Endothelium-dependent and--independent relaxation by acetylcholine and the nitric oxide donor diethylamine/NO, respectively, did not depend on gender and were equally suppressed by LPS treatment. Treatment with LPS accentuated the effects of oxyhemoglobin in rings with and without endothelium and this was significantly larger in rings from females compared to males. Thus, gender differences were present in the effect of LPS on vascular responses. LPS induced a greater inhibition of depolarization by potassium chloride, but not receptor-activated contractions, in aortic rings from female verus male rats.
Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, TX, USA.
T
T
Okawa
Y P
YP
Vedernokov
G R
GR
Saade
R E
RE
Garfield
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular; D005260:Female; D006090:Gram-Negative Bacteria; D006834:Hydrazines / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D009569:Nitric Oxide / Q000096:biosynthesis; D009589:Nitrogen Oxides; D010108:Oxyhemoglobins / Q000494:pharmacology; D010656:Phenylephrine; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012723:Sex; D012772:Shock, Septic / Q000503:physiopathology
D006834:Hydrazines; D008070:Lipopolysaccharides; D009589:Nitrogen Oxides; D010108:Oxyhemoglobins; D010656:Phenylephrine; D009569:Nitric Oxide; D011189:Potassium Chloride; C084012:1,1-diethyl-2-hydroxy-2-nitrosohydrazine; D000109:Acetylcholine