In vivo site-directed mutagenesis of yeast plasmids using a three-fragment homologous recombination system.
Kitagawa
Katsumi
K
Department of Molecular Pharmacology, St. Jude Children's Resarch Hospital, Memphis, TN 38105, USA. katsumi.kitagawa@stjude.org
Abdulle
Rashid
R
D017931:DNA Primers / Q000235:genetics; D005800:Genes, Fungal; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011995:Recombination, Genetic; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012680:Sensitivity and Specificity; D017385:Sequence Homology; D015003:Yeasts / Q000235:genetics*
D017931:DNA Primers