Induction of light emission by luminescent bacteria treated with UV light and chemical mutagens.
Intensity of light emission by luminescent bacteria in response to UV irradiation and chemical mutagens was tested. We demonstrated that luminescence of six strains of marine bacteria (belonging to four species: Photobacterium leiognathi, P. phosphoreum, Vibrio fischeri and V. harveyi) is significantly increased by UV irradiation relatively shortly after dilution of cultures. Such a stimulation of luminescence was abolished in cells treated with chloramphenicol 15 min before UV irradiation, indicating that effective gene expression is necessary for UV-mediated induction of light emission. These results suggest that stimulation of luminescence in UV-irradiated bacterial cells may operate independently of the quorum sensing regulation. A significant induction of luminescence was also observed upon treatment of diluted cultures of all investigated strains with chemical mutagens: sodium azide (SA), 2-methoxy-6-chloro-9-(3-(2-chloroethyl)aminopropylamino)acridine x 2HCl (ICR-191), 4-nitro-o-phenylenediamine (NPD), 4-nitroquinolone-N-oxide (NQNO), 2-aminofluorene (2-AF), and benzo[alpha]pyrene. These results support the proposal that genes involved in bioluminescence belong to the SOS regulon. The use of bacterial luminescence systems in assays for detection of mutagenic compounds is discussed in the light of this proposal.
Laboratory of Molecular Biology, University of Gdańsk, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Gdańsk, Poland.
Agata
A
Czyz
Konrad
K
Plata
Grzegorz
G
Wegrzyn
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D015964:Gene Expression Regulation, Bacterial; D008027:Light; D008163:Luminescent Measurements; D016254:Mutagenesis, Insertional; D009153:Mutagens / Q000494:pharmacology*; D018462:Photobiology; D010957:Plasmids / Q000235:genetics; D013014:SOS Response, Genetics; D014466:Ultraviolet Rays; D014733:Vibrio / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*
D001426:Bacterial Proteins; D009153:Mutagens