[Use of molecular biology methods for diagnosing fungal infections].
A total of 130 various clinical materials taken from 48 children with suspected systemic fungal infection were used for the study. Clinical samples were tested by use of classical mycological procedures well as by use of molecular technique (PCR assay). The fragments of 125-bp (EO3) and 317 bp (HSP) specific for C. albicans were used for amplification. Fifty seven samples (48%) were positive for Candida albicans and eighty four (68%) by use of PCR. It should be stressed that 4 blood samples, 21 urine samples and 5 other samples were positive by use of molecular technique, only. PCR is sensitive and rapid method for detection and identification of Candida albicans from clinical materials of children with fungal infection. This technique can be applied for monitoring presence of fungal DNA in tested samples during antifungal therapy.
Zakład Mikrobiologii Klinicznej Instytutu Pomnik-Centrum Zdrowia Dziecka w Warszawie.
Barbara
B
Garczewska
Danuta
D
Dzierzanowska
D001769:Blood / Q000382:microbiology; D002176:Candida albicans / Q000302:isolation & purification; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology; D002648:Child; D005821:Genetic Techniques; D006801:Humans; D009181:Mycoses / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D016133:Polymerase Chain Reaction; D014556:Urine / Q000382:microbiology