Molecular cloning and characterization of human DDX36 and mouse Ddx36 genes, new members of the DEAD/H box superfamily.
With the strategy of homologue molecular cloning using the sequence of the maleless gene (mle) of Drosophila, the novel homologous human and mouse genes with longer DNA/RNA helicase box (DEAD/DEAH box), named DDX36 and Ddx36 genes, respectively, were cloned as new members of the DEAD/H box superfamily. The predicted protein encoded by human DDX36 gene has a sequence identity of 37% and similarity of 58% with the MLE protein of Drosophila and 91% and 94% with the predicted protein encoded by mouse Ddx36 gene, respectively. Northern blotting of DDX36 shows a single strong signal of 3.8 kb in the hybridization pattern in human testis but no or very weak signal in other tissues. The DDX36 gene is mapped to chromosome 3q25.1-3q25.2, in which 26 exons and 25 introns have been identified. DDX36 and Ddx36 genes may be involved in sex development, spermatogenesis and male reproduction.
Fu
Jun-Jiang
JJ
Laboratory of Human Reproductive Engineering, Central South University Xiangya Medical College, Changsha 410078, China. lgxdirector@sina.com
Li
Lu-Yun
LY
Lu
Guang-Xiu
GX
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002868:Chromosomal Proteins, Non-Histone; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D053487:DEAD-box RNA Helicases; D004265:DNA Helicases / Q000145:classification / Q000235:genetics*; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D020365:RNA Helicases / Q000145:classification / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics
D002868:Chromosomal Proteins, Non-Histone; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D014157:Transcription Factors; C070837:mle protein, Drosophila; C466988:DHX36 protein, human; C466989:Dhx36 protein, mouse; D004265:DNA Helicases; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases