[Some peculiarities of DNA structure of cyanophage LPP-3].
The efficiency of radioactive labeling of 3'- and 5'-ends of cyanophage LPP-3 DNA by polynucleotide kinase T4 and terminal transferase under various reaction conditions has been investigated. The obtained data prove that cyanophage LPP-3 DNA has the protruding 3'-ends. The experiments on ligation of native molecules of LPP-3 DNA evidence that the virus genome ends do not display any complimentarity. Separate fragments of LPP-3 DNA were cloned. The restriction analysis of the cloned fragments has confirmed a supposition on the absence of LPP-3 cyanophage of GGGCC and GGCCC sequences in the genome. A hypothesis has been suggested about similar site-specificity of the virus. Counterselection of the genome LPP-3 cyanophage allows it to be considered a promising one in the construction of new cloning vectors in cyanobacterium.
Syrchin
S A
SA
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Mendzhul
M I
MI
D001435:Bacteriophages / Q000235:genetics*; D001665:Binding Sites; D000458:Cyanobacteria / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry*; D016679:Genome, Viral; D015183:Restriction Mapping
D004279:DNA, Viral