N4 RNA polymerase II, a heterodimeric RNA polymerase with homology to the single-subunit family of RNA polymerases.
Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4 early promoter Pe3. These genes code for proteins of 269 and 404 amino acid residues with sequence similarity to the single-subunit, phage-like RNA polymerases. The genes encoding the N4 RNAPII subunits, as well as a synthetic construct encoding a fusion polypeptide, have been cloned and expressed. Both the individually expressed subunits and the fusion polypeptide reconstitute functional enzymes in vivo and in vitro.
Willis
S H
SH
Department of Molecular Genetics, The University of Chicago, Chicago, Illinois 60637, USA.
Kazmierczak
K M
KM
Carter
R H
RH
Rothman-Denes
L B
LB
D000595:Amino Acid Sequence; D025522:Bacteriophage N4 / Q000201:enzymology* / Q000502:physiology; D012321:DNA-Directed RNA Polymerases / Q000145:classification / Q000235:genetics; D019281:Dimerization; D004926:Escherichia coli / Q000254:growth & development / Q000821:virology*; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases