The Epstein-Barr virus immediate-early protein BZLF1 induces both a G(2) and a mitotic block.
The Epstein-Barr virus immediate-early protein BZLF1 is a transcriptional activator that mediates the switch from latent to lytic infection. Here we demonstrate that BZLF1 induces both a G(2) block and a mitotic block in HeLa cells and inhibits chromosome condensation. While the G(2) block is associated with decreased cyclin B1 in host cells and can be rescued by overexpression of cyclin B1, the mechanism for the mitotic defect is as yet undetermined.
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7295, USA.
Amy
A
Mauser
Elizabeth
E
Holley-Guthrie
Dennis
D
Simpson
William
W
Kaufmann
Shannon
S
Kenney
D019926:Cyclin B / Q000502:physiology; D056744:Cyclin B1; D004268:DNA-Binding Proteins / Q000502:physiology*; D016195:G2 Phase; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D015534:Trans-Activators / Q000502:physiology*; D014764:Viral Proteins
C063676:BZLF1 protein, Herpesvirus 4, Human; C534699:CCNB1 protein, human; D019926:Cyclin B; D056744:Cyclin B1; D004268:DNA-Binding Proteins; D015534:Trans-Activators; D014764:Viral Proteins