A rapid, nongenomic action of glucocorticoids in rat B103 neuroblastoma cells.
We report here a new example in which glucocorticoids (GCs) acted in a rapid, nongenomic way. In rat B103 neuroblastoma cells, 5-hydroxytryptamine (5-HT) was found to evoke an immediate rise in intracellular free calcium concentration ([Ca(2+)](i)). Pre-incubation of B103 cells for 5 min with corticosterone (B) or bovine serum albumin-conjugated corticosterone (B-BSA) concentration-dependently (10(-4)-10(-8) M) inhibited the peak increments in [Ca(2+)](i). Cortisol and dexamethasone had a similar effect, while deoxycorticosterone and cholesterol were ineffective. This rapid inhibitory effect of corticosterone could be mimicked by protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) and abolished completely by PKC inhibitors Ro31-8220 or GF-109203X. Neither pertussis toxin (PTX) nor nuclear GC receptor (GR) antagonist RU38486 influenced the rapid action of B. Our results suggest that GCs can modulate the 5-HT-induced Ca(2+) response in B103 cells in a membrane-initiated, nongenomic, and PKC-dependent manner.
Neuroscience Research Institute and Department of Neurobiology, Second Military Medical University, 800 Xiangyin Road, 200433 Shanghai, China.
Jian-Zhong
JZ
Han
Wen
W
Lin
Shu-Jie
SJ
Lou
Jian
J
Qiu
Yi-Zhang
YZ
Chen
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004347:Drug Interactions; D005938:Glucocorticoids / Q000494:pharmacology*; D009447:Neuroblastoma / Q000378:metabolism*; D051381:Rats; D012701:Serotonin / Q000494:pharmacology*; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D005938:Glucocorticoids; D012701:Serotonin; D002118:Calcium