Flow cytometric assay of platelet glycoprotein receptor numbers in hypercholesterolemia.
In this study, platelet glycoprotein (Gp) receptor numbers were measured by a flow cytometric assay using Cytoquant Gp in seven hypercholesterolemic and five normal subjects. Thrombin receptor agonist peptide (TRAP) was used to activate platelets. In hypercholesterolemia the Gp receptor numbers per resting platelet were found to be: 38,629 +/- 8,538 (GpIIb/IIIa), 22,269 +/- 5,628 (GpIb), 37,037 +/- 9,810 (GpIIIa), 224 +/- 504 (CD62-P). After activation, receptor numbers were determined to be: 56,399 +/- 9,003 (GpIIb/IIIa), 10,970 +/- 5,319 (GpIb), 50,715 +/- 7,904 (GpIIIa), 1,222 +/- 687 (P-selectin). In the normal group before the activation, receptor numbers were: 43,828 +/- 8,862 (GpIIb/IIIa), 22,166 +/- 3,847 (GpIb), 42,351 +/- 1,049 (GpIIIa), 62 +/- 139 (CD62-P), After activation, receptor numbers were determined to be: 60,573 +/- 4,294 (GpIIb/IIIa), 13,003 +/- 4,118 (GpIb), 52, 067 +/- 1,039 (GpIIIa), 3,608 +/- 1,508 (CD62-P). In hypercholesterolemic subjects, GpIIb/IIIa and GpIIIa receptor numbers on activated platelets increased significantly, whereas P-selectin numbers remained unchanged. However, the GpIb levels decreased significantly. In the control group, after activation, GpIIb/IIIa and P-selectin receptors increased significantly, GpIIIa receptor numbers did not change significantly, whereas GpIb receptor numbers decreased significantly. When the GpIIb/IIIa, GpIb, GpIIIa receptor numbers of the control group and hypercholesterolemic group were compared before and after activation, no significant changes were observed (P > 0.05). But P-selectin receptor numbers were significantly decreased in hypercholesterolemic patients compared to normals following TRAP activation (P < 0.05). In this study, the effect of hypercholesterolemia on platelet function was observed. The striking observation about present study was the marked decrease in P-selectin expression after activation in the hypercholesterolemics compared to normals. This finding suggests some sort of platelet dysfunction in these individuals.
Ozsavci
Derya
D
Department of Biochemistry, Marmara University Faculty of Pharmacy, Tibbiye Caddesi, No. 49, Haydarpasa 81010 Kadikoy, Istanbul, Turkey. deryaozsavci@hotmail.com
Yardimci
Turay
T
Demirel
Gulderen Yanikkaya
GY
Demiralp
Emel
E
Uras
Fikriye
F
Onder
Ekin
E
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005434:Flow Cytometry; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood*; D039661:Integrin beta3 / Q000097:blood; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis*; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000032:analysis*; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*
D039661:Integrin beta3; D019007:P-Selectin; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019038:Platelet Glycoprotein GPIb-IX Complex; D010980:Platelet Membrane Glycoproteins; C032193:glycoprotein receptor GPIb-IX