Surgical stress during operation for gastrointestinal cancer increases plasma thioredoxin levels and decreases mitochondrial membrane potential in peripheral blood lymphocytes.
Surgical stress is difficult to evaluate quantitatively. It has been reported that mitochondrial membrane potential (delta psi(m)) in the peripheral blood lymphocytes (PBLs) is decreased by surgical stress. Thioredoxin (TRX), a small protein with redox-active dithiol/disulfide in the active site, is induced by a variety of oxidative stresses and secreted from the cells. Accumulating evidence shows that plasma levels of TRX are elevated in oxidative stress-associated disorders. In the present study, we examined plasma levels of TRX in cases undergoing operations for gastrointestinal cancer. Plasma levels of TRX were significantly elevated on the first postoperative day compared with the pre-operative levels. The changes in the plasma TRX levels tended to show an inverse relationship with the changes in delta psi(m) in PBLs, which shows a significant decrease caused by surgical stress. Plasma TRX levels as well as delta psi(m) in PBLs are valuable markers to evaluate surgical stress.
Department of Surgery, Kitano Medical Research Institute and Hospital, 13-3 Kamiyama-cho, Kita-ku, Osaka 530-8480, Japan.
Kazuki
K
Sugawara
Hajime
H
Nakamura
Michiyuki
M
Kanai
Shingo
S
Iwata
Yasuhiro
Y
Kawai
Yoshiro
Y
Taki
Junji
J
Yodoi
Arimichi
A
Takabayashi
D005434:Flow Cytometry / Q000379:methods; D005770:Gastrointestinal Neoplasms / Q000097:blood / Q000503:physiopathology / Q000601:surgery*; D006801:Humans; D007425:Intracellular Membranes / Q000502:physiology*; D008113:Liver Neoplasms / Q000097:blood / Q000503:physiopathology / Q000556:secondary / Q000601:surgery; D008214:Lymphocytes / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D008928:Mitochondria / Q000502:physiology*; D016343:Monitoring, Intraoperative / Q000379:methods; D010084:Oxidation-Reduction; D012016:Reference Values; D013312:Stress, Physiological / Q000097:blood / Q000503:physiopathology*; D013879:Thioredoxins / Q000097:blood*
D013879:Thioredoxins