Preparative isolation and purification of calycosin from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography.
Calycosin was purified from an ethyl acetate extract of the root of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography. The separation was performed in two steps with a two-phase solvent system composed of n-hexane-chloroform-methanol-water (1:3:3:2, v/v). From 200 mg of the crude extract, 14.8 mg of calycosin was obtained at over 99% purity as determined by HPLC analysis, and its chemical structure was confirmed by MS, 1H and 13C nuclear magnetic resonance.
Peking University Modern Research Center for Traditional Chinese Medicine, Beijing 100083, China.
Xiaofeng
X
Ma
Tianyou
T
Zhang
Yun
Y
Wei
Pengfei
P
Tu
Yingjie
Y
Chen
Yoichiro
Y
Ito
D027883:Astragalus Plant / Q000737:chemistry*; D027885:Astragalus propinquus; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D007529:Isoflavones / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy
D007529:Isoflavones; C121707:7,3'-dihydroxy-4'-methoxyisoflavone