Comparison of bone remodeling indicators in climacteric women.
OBJECTIVE
The objective was to study the polymorphism of collagen type 1 alpha 1 (COL1 A1) gene as a genetic marker, the biochemical markers of formation (osteocalcin) and bone reabsorption (cross-links of pyridinoline-CTx), bone mineral density and bone ultrasonometry compared to bone densitometry (DEXA).


METHODS
The study included 82 women ranging in age from 45 to 60 years, menopausal from 1 to 10 years. Polymorphism of COL1 A1 was assessed by PCR (polymerase chain reaction), by the specific allele technique. Bone formation markers were studied using an ELISA (Novocalcin and Active Crosslaps). A Hologic 4500 A QDR (DEXA) densitometer and DBM Sonic 1200 IGEA ultrasonograph were employed.


RESULTS
The bone reabsorption marker (cross-links of pyridinoline-CTx) demonstrated statistically significant negative correlation with bone mineral density (lumbar and femoral neck), while the bone formation marker (osteocalcin) did not display a correlation with bone mineral density. Bone ultrasonometry yielded a statistically significant positive correlation with bone densitometry. Collagen type 1 alpha 1 polymorphism was not identified by the technique employed.


CONCLUSIONS
The bone reabsorption marker (cross-links of pyridinoline) and bone ultrasonometry and densitometry are measurements enabling evaluation of bone remodeling.
Department of Obstetrics and Gynecology, School of Medicine, University of São Paulo, Brazil.
Paulo Augusto de Almeida
PA
Junqueira
Angela Maggio
AM
da Fonseca
Vicente Renato
VR
Bagnoli
Daniel
D
Giannella-Netot
Cristovao Luis Pitangueira
CL
Mangueira
Cassio Negro
CN
Coimbra
José Aristodemo
JA
Pinotti
D000596:Amino Acids / Q000378:metabolism; D015519:Bone Density; D016723:Bone Remodeling; D001862:Bone Resorption / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D002979:Climacteric; D003094:Collagen / Q000235:genetics* / Q000378:metabolism; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D003720:Densitometry; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008593:Menopause; D008875:Middle Aged; D015675:Osteocalcin / Q000378:metabolism; D015663:Osteoporosis, Postmenopausal / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D000596:Amino Acids; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D005819:Genetic Markers; D015675:Osteocalcin; C015484:pyridinoline; D003094:Collagen