Occupational exposure to pesticides and cytogenetic damage: results of a Hungarian population study using the micronucleus assay in lymphocytes and buccal cells.
The frequency of micronuclei (MN) in peripheral blood lymphocytes and in buccal epithelial cells was used as a biomarker of genotoxic effects resulting from occupational exposure to pesticides. In addition, the cytokinesis-block proliferation index (CBPI) was calculated to detect possible variations in the proliferative kinetics of lymphocytes due to pesticide exposure. This study was performed on 84 pesticide-exposed workers and 65 unexposed controls from Hungary. The pesticide-exposed workers, classified as moderately and highly exposed, were also evaluated separately. Statistical evaluation of the cytogenetic biomarkers indicated that there were no significant differences between pesticide-exposed workers and controls, nor between moderately and highly exposed workers. Nevertheless, the statistical analysis revealed that additional factors such as age, sex, ingestion of raw vegetables, and working as a pesticide applicator affected lymphocyte MN frequency. In addition, age, sex, and smoking affected the frequency of MN in buccal cells. Results from the CBPI analysis showed that the proliferation index decreased with pesticide exposure and that this parameter was also affected by smoking and by the gender of individuals. The results of this study indicate no significant increase in MN in this group of Hungarian workers; however, the reduced CBPI in the highly exposed population suggests a possible genotoxic effect of pesticide exposure.
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici Cn, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Susana
S
Pastor
Amadeu
A
Creus
Noel
N
Xamena
Csaba
C
Siffel
Ricard
R
Marcos
D000328:Adult; D002478:Cells, Cultured; D004249:DNA Damage; D005260:Female; D006801:Humans; D006814:Hungary; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009061:Mouth Mucosa / Q000187:drug effects; D016273:Occupational Exposure / Q000009:adverse effects*; D010575:Pesticides / Q000633:toxicity*; D012016:Reference Values; D012044:Regression Analysis; D012307:Risk Factors
D010575:Pesticides