Isolation and characterization of a strain of Bacillus thuringiensis ssp. kurstaki containing a new delta-endotoxin gene.
A strain of Bacillus thuringiensis that showed significantly high toxicity to Plutella xylostella and Spodoptera exigua was isolated from a Korean soil sample and characterized. The isolate, named B. thuringiensis K1, was determined to belong to ssp. kurstaki (H3a3b3c) type by an H antisera agglutination test and produced bipyramidal inclusions. Plasmid pattern of K1 was different from that of the reference strain, ssp. kurstaki HD-1, but the parasporal inclusion protein profile of K1 had two major bands that were similar in size to those of ssp. kurstaki HD-1. To verify the delta-endotoxin gene types of K1, PCR analysis with specific cry gene primers was performed to show that K1 contained a new cry gene in addition to cry1Aa, cry1Ab, cry1Ac, cry1E and cry2 genes. PCR-amplified region of the new cry gene, cryX, showed 79% similarity to cry1Fa1 gene (GenBank Accession No. M63897). In an insect toxicity assay, K1 had higher toxicity against Plutella xylostella and S. exigua than ssp. kurstaki HD-1.
Li
M S
MS
School of Agricultural Biotechnology, Seoul National University, 103 Seodoon-Dong, Suwon 441-744, Korea.
Je
Y H
YH
Lee
I H
IH
Chang
J H
JH
Roh
J Y
JY
Kim
H S
HS
Oh
H W
HW
Boo
K S
KS
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000302:isolation & purification; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology / Q000633:toxicity; D001427:Bacterial Toxins; D001483:Base Sequence; D004731:Endotoxins / Q000235:genetics* / Q000494:pharmacology / Q000633:toxicity; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D007306:Insecticides / Q000633:toxicity; D008018:Life Cycle Stages / Q000187:drug effects; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D018411:Spodoptera / Q000187:drug effects
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C084957:cryIX protein, Bacillus thuringiensis; C030956:insecticidal crystal protein, Bacillus Thuringiensis