Up-scaling capillary zone electrophoresis separations of polydisperse anionic polyelectrolytes with preparative free-flow electrophoresis exemplified with a soil fulvic acid.
A scale-up of analytical capillary zone electrophoresis (CZE) to preparative free-flow electrophoresis (FFE) is described. FFE allows fractionations based on charge densities in larger amounts than in CZE, enabling further off-line analysis of the fractions. Model compounds (carboxylic acids and polystyrene sulfonates) showed a similar behavior in FFE as in CZE. Diffusion and electrodynamic distortion effects are more pronounced in FFE than in CZE. A soil fulvic acid was analyzed by CZE and fractionated by FFE. A comparison of the FFE fractions with CZE measurements of the same sample using the effective mobility scale showed good agreement of the two methods.
Institute for Soil Ecology, Forschungszentrum für Umwelt und Gesundheit, Neuherberg, Germany.
Jens
J
Junkers
Philippe
P
Schmitt-Kopplin
Jean-Charles
JC
Munch
Antonius
A
Kettrup
D001578:Benzopyrans / Q000032:analysis / Q000302:isolation & purification*; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes; D006812:Humic Substances / Q000032:analysis / Q000302:isolation & purification; D007202:Indicators and Reagents; D000071228:Polyelectrolytes; D011108:Polymers / Q000032:analysis / Q000302:isolation & purification*; D012987:Soil
D001578:Benzopyrans; D005456:Fluorescent Dyes; D006812:Humic Substances; D007202:Indicators and Reagents; D000071228:Polyelectrolytes; D011108:Polymers; D012987:Soil; C005023:fulvic acid