Chicken model for studying dietary antioxidants reveals that apple (Cox's Orange)/broccoli (Brassica oleracea L. var. italica) stabilizes erythrocytes and reduces oxidation of insoluble muscle proteins and lipids in cooked liver.
A chicken model for studying the effects of antioxidants in the diet on oxidative status was set up. Chickens fed a semi-synthetic diet low in antioxidants showed a remarkable decrease in erythrocyte stability toward H(2)O(2) or 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH), but increases in catalase activity in liver, carbonyls in insoluble muscle proteins, and enhanced lipid oxidation in heat-treated liver samples compared to that of conventionally fed chickens. Thus, this chicken model proved to be more susceptible to oxidative changes than conventionally fed chickens, reflecting a low antioxidative defense. Supplementing this low antioxidant diet with 10% apple/broccoli mixture counteracted these changes, except for activity of catalase in the liver and AAPH-induced lysis of erythrocytes. Supplementation with 10% sweet corn only reduced the carbonyl content in insoluble proteins. However, neither low antioxidant diet nor vegetable supplements affected selected antioxidative enzymes or oxidative stability of lipids in heat-treated muscle tissue.
Young
Jette F
JF
Danish Institute of Agricultural Sciences, Department of Animal Product Quality, Research Centre Foulum, Tjele, Denmark. JetteF.Young@agrsci.dk
Steffensen
Charlotte L
CL
Nielsen
Jacob H
JH
Jensen
Søren K
SK
Stagsted
Jan
J
D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001937:Brassica / Q000737:chemistry*; D002645:Chickens; D004032:Diet; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000737:chemistry*; D027845:Malus / Q000737:chemistry*; D009124:Muscle Proteins / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014810:Vitamin E / Q000032:analysis / Q000097:blood
D000975:Antioxidants; D009124:Muscle Proteins; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E