Isolation and characterization of the ethynylestradiol-biodegrading microorganism Fusarium proliferatum strain HNS-1.
We cultivated hundreds of sediment, soil, and manure samples taken from rivers and farms in a medium containing ethynylestradiol (EE2) as the sole source of carbon, so that microorganisms in the samples would acclimatize to the presence of EE2. Finally, we isolated an EE2-degrading microorganism, designated as strain HNS-1, from a cowshed sample. Based on its partial nucleotide sequence (563 bp) of the 28S rRNA gene, strain HNS-1 was identified as Fusarium proliferatum. Over 15 days, F. proliferatum strain HNS-1 removed 97% of EE2 at an initial concentration of 25 mg.L-1, with a first-order rate constant of 0.6 d-1. Unknown products of EE2 degradation, which may be more polar compounds that have a phenolic group, remained in the culture medium.
Department of Chemical Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka, Koganei, Tokyo 184-8588, Japan. shijiang@cc.tuat.ac.jp
J H
JH
Shi
Y
Y
Suzuki
B D
BD
Lee
S
S
Nakai
M
M
Hosomi
D000383:Agriculture; D000818:Animals; D000829:Animals, Domestic; D001673:Biodegradation, Environmental; D019149:Bioreactors; D045166:Estradiol Congeners / Q000378:metabolism*; D004997:Ethinyl Estradiol / Q000378:metabolism*; D005670:Fusarium / Q000302:isolation & purification* / Q000502:physiology; D019015:Geologic Sediments / Q000382:microbiology; D007700:Kinetics; D008372:Manure / Q000382:microbiology; D012988:Soil Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D045166:Estradiol Congeners; D008372:Manure; D014874:Water Pollutants, Chemical; D004997:Ethinyl Estradiol