[Experimental study on microvascular architecture of human and rabbit flaps with two methods].
OBJECTIVE
To study effective method of showing the microvascular architecture of human and rabbit skin flaps.


METHODS
According to endogenous alkaline phosphatase activity, Gomori-Takamatsu method and NBT/BCIP method were used to observe the microvascular architecture.


RESULTS
Both methods had capability to demonstrate the microvascular architecture of the human skin flap, whereas the examining results of rabbits were negative.


CONCLUSIONS
Great difference of endogeneous alkaline phosphatase activity exists in different animals. Gomori-Takamatsu method and NBT/BCIP method are useful in study of microvascular architecture of human skin flaps.
Zhang
Hongwei
H
China-Japan Friendship Hospital, Beijing 100029, China.
Pan
Lin
L
Wang
Hui
H
Tian
Xiaochen
X
Cai
Zhe
Z
Song
Yeguang
Y
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D006801:Humans; D008297:Male; D008833:Microcirculation / Q000033:anatomy & histology; D011817:Rabbits; D013524:Surgical Flaps / Q000098:blood supply*
D000469:Alkaline Phosphatase