A DNA-electrochemical biosensor for screening environmental damage caused by s-triazine derivatives.
An electrochemical DNA-biosensor has been used to investigate the interactions between DNA and members of a group of ten derivatives of 1,3,5-triazine herbicides: chloro-s-triazines (atrazine, propazine, terbutylazin, and cyanazin), thiomethyl-s-triazines (ametryn, prometryn, terbutryn, and simetryn), and methoxy-s-triazines (prometon and terbumeton). A UV spectrophotometric study of this group of herbicides was also undertaken. Of this group only cyanazin could be oxidized in aqueous solution using a glassy carbon electrode. Use of the electrochemical DNA-biosensor revealed the occurrence of a time-dependent interaction of all the herbicides with DNA, via the appearance of guanine, guanosine, and adenosine oxidation signals that correspond to DNA damage. Adduct formation between the herbicide and the DNA purine bases guanine and adenine is suggested as a mechanism.
Oliveira-Brett
Ana Maria
AM
Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, 3004-535 Coimbra, Portugal. brett@ci.uc.pt
da Silva
Luís Antônio
LA
D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis* / Q000187:drug effects; D004249:DNA Damage; D004563:Electrochemistry / Q000295:instrumentation*; D004784:Environmental Monitoring / Q000295:instrumentation*; D006540:Herbicides / Q000032:analysis* / Q000633:toxicity; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines
D006540:Herbicides; D014227:Triazines; D004247:DNA