Manifestation of Y-chromosomal deletions in the human testis: a morphometrical and immunohistochemical evaluation.
BACKGROUND
Deletions of the AZF (azoospermia factor) subregions on the Y chromosome are accompanied by a diverse spectrum of spermatogenic disturbances ranging from hypospermatogenesis to total depletion of germ cells causing infertility. The AZF region encodes gene products which are candidates for the genetic control of spermatogenesis. Although it is known which genes are involved, a general principle of cause and effect cannot yet be deciphered and the deletion type has non-uniform histological phenotypes.


RESULTS
We analysed morphological parameters of testicular biopsies from 17 patients diagnosed for Y chromosome microdeletions. As control groups we analysed testes from patients with idiopathic Sertoli cell-only (SCO) syndrome (n = 11), mixed atrophy (n = 10) and complete spermatogenesis (n = 11). A detailed genetic analysis on the extension of the observed microdeletions revealed similar breakpoints in the distal and proximal region of the AZFc region, indicating a common mechanism of homologous recombination for such deletions, as has been suggested before. Morphometric parameters such as the diameter of the tubules, lumen, thickness of the lamina propria and height of the tubule epithelia were investigated. The diameter of the tubules from patients with microdeletions was found to be significantly smaller compared with patients with mixed atrophy. Considering also the size of the tubules, lumen and epithelia, a Y-chromosomal microdeletion represents an intermediate state between an idiopathic SCO and normal spermatogenesis. The immunohistochemical analysis of six different Sertoli cell markers, cytokeratin 18, vimentin, inhibin alpha subunit, 14-3-3 theta, FSH receptor and androgen receptor, revealed no impact of AZF deletion on the specific expression pattern of these genes.


CONCLUSIONS
Our results suggest that, notwithstanding the deletion of a common region in the AZFc region, microdeletions of the Y chromosome lead to an intermediate status between idiopathic SCO and complete spermatogenesis, resulting in a heterogeneous histological profile regardless of the seminiferous activity. The Sertoli cell function seems not to be altered.
Institute of Reproductive Medicine, Westphalian Wilhelms-University, Domagkstr. 11, D-48129 Muenster, Germany.
C M
CM
Luetjens
J
J
Gromoll
M
M
Engelhardt
S
S
Von Eckardstein
M
M
Bergmann
E
E
Nieschlag
M
M
Simoni
D000328:Adult; D001284:Atrophy; D015415:Biomarkers; D041322:Chromosomes, Human, Y; D017353:Gene Deletion; D006801:Humans; D007150:Immunohistochemistry; D007248:Infertility, Male / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D012708:Sertoli Cells / Q000378:metabolism / Q000473:pathology; D013091:Spermatogenesis; D013737:Testis / Q000473:pathology* / Q000503:physiopathology*
D015415:Biomarkers