A mycorrhiza-responsive protein in wheat roots.
A small protein, designated Myk15, was found to be strongly induced in wheat ( Triticum aestivum) roots colonized by the arbuscular mycorrhizal fungus Glomus intraradices. This protein, which is most abundant in root fractions characterized by strong mycorrhizal colonization, has been characterized using two-dimensional polyacrylamide gel electrophoresis and microsequencing. It has an apparent molecular mass of 15 kDa and an isoelectric point of 4.5. The N-terminal sequence has high similarity to a peptide sequence deduced from an expressed sequence tag (EST) clone derived from Medicago truncatula roots colonized by G. intraradices. This EST clone is predicted to code for a protein with a similar size and isoelectric point as Myk15. The N-terminus of the deduced M. truncatula protein contains a highly hydrophobic stretch of 24 amino acid residues preceding the region with high similarity to the Myk15 N-terminus. This hydrophobic stretch is predicted to form a transmembrane alpha-helix and may correspond to a cleavable targeting domain.
Leibniz-Institut für Pflanzenbiochemie, Abteilung Sekundärstoffwechsel, Weinberg 3, 06120 Halle (Saale), Germany. tfester@ipb-halle.de
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D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D029902:Medicago / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology*; D016415:Sequence Alignment; D014908:Triticum / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*
D008565:Membrane Proteins; C470809:Myk15 protein, Triticum aestivum; D010940:Plant Proteins