[Experimental study of multidrug resistance mediated by human laminin receptor in gastric cancer cells].
OBJECTIVE
To study the mechanisms of multidrug resistance (MDR) mediated by human 67 000 laminin receptor (LR) with a relative molecular mas of 67 000 in gastric cancer cells.


METHODS
Antisense RNA expression vector corresponding to LR precursor (LRP) was constructed by DNA recombinant technique, and transferred into gastric cancer MDR cells SGC7901/VCR with Lipofect AMINE. Western blot was employed to determine the LR expression level in gastric cancer cells. The sensitivity of gastric cancer cells to chemotherapeutic drugs was evaluated with MTT assay. Flow cytometry was used to analyze the cell cycle and to assess the mean fluorescence intensity of intracellular adriamycin in gastric cancer cells.


RESULTS
Western blotting analysis demonstrated a decreased expression level of LR in SGC7901/VCR cells transfected with LRP antisense RNA expression vector. In comparison with the gastric cancer cells with out transfection or transfected with invalid vector, LR down-regulated transfectants (SGC7901/VCR-anLRP) showed higher sensitivity to vincristine, adriamycin, 5-fluodrouracil and cisplatin, and increased accumulation and retention of adriamycin. Cell cycle analysis suggested G1 block and spontaneous apoptosis of SGC7901/VCR-anLRP cells.


CONCLUSIONS
LR might take part in mediation of MDR in gastric cancer cells through interfering with drug accumulation and cell apoptosis.
Shi
Yongquan
Y
Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Zhai
Huihong
H
Wang
Xin
X
Ning
Xiaoxuan
X
Zhao
Yanqiu
Y
Fan
Daiming
D
D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D016193:G1 Phase; D006801:Humans; D017474:Receptors, Laminin / Q000032:analysis / Q000502:physiology*; D013274:Stomach Neoplasms / Q000737:chemistry / Q000188:drug therapy* / Q000473:pathology; D014162:Transfection
D017474:Receptors, Laminin