A solid-state NMR study of phospholipid-cholesterol interactions: sphingomyelin-cholesterol binary systems.
We used solid-state NMR techniques to probe the interactions of cholesterol (Chol) with bovine brain sphingomyelin (SM) and for comparison of the interactions of Chol with dipalmitoylphosphatidylcholine (DPPC), which has a similar gel-to-liquid crystalline transition temperature. (1)H-, (31)P-, and (13)C-MASNMR yielded high-resolution spectra from multilamellar dispersions of unlabeled brain SM and Chol for analysis of chemical shifts and linewidths. In addition, (2)H-NMR spectra of oriented lipid membranes with specific deuterium labels gave information about membrane ordering and mobility. Chol disrupted the gel-phase of pure SM and increased acyl chain ordering in the liquid crystalline phase. As inferred from (13)C chemical shifts, the boundaries between the ordered and disordered liquid crystalline phases (L and L) were similar for SM and DPPC. The solubility limit of Chol in SM was ~50 mol %, the same value as previously reported for DPPC membranes. We found no evidence for specific H-bonding between Chol and the amide group of SM. The order parameters of a probe molecule, d31-sn1-DPPC, in SM were slightly higher than in DPPC for all carbons except the terminal groups at 30 mol % but were not significantly different at 5 and 60 mol % Chol. These studies show a general similarity with some subtle differences in the way Chol interacts with DPPC and SM. In the environment of a typical biomembrane, the higher proportion of saturated fatty acyl chains in SM compared to other phospholipids may be the most significant factor influencing interactions with Chol.
Guo
Wen
W
Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118 USA.
Kurze
Volker
V
Huber
Thomas
T
Afdhal
Nezam H
NH
Beyer
Klaus
K
Hamilton
James A
JA
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D001921:Brain / Q000378:metabolism; D002417:Cattle; D002784:Cholesterol / Q000737:chemistry*; D006860:Hydrogen Bonding; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010743:Phospholipids / Q000737:chemistry*; D011485:Protein Binding; D013109:Sphingomyelins / Q000737:chemistry*; D013696:Temperature
D008055:Lipids; D010743:Phospholipids; D013109:Sphingomyelins; D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol