Free radical scavenging properties of sulfinpyrazone.
Sulfinpyrazone, a potent uricosuric drug, was tested in vitro for its scavenging action against oxygen free radicals. In this study, sulfinpyrazone was able to scavenge 1,1-diphenyl-2-picrylhydrazyl radical with IC50 value of 29.82 micrograms/ml compared to butylated hydroxytoluene (BHT, IC50 value = 20.15 micrograms/ml) and Trolox (IC50 value = 16.01 micrograms/ml). It was able to scavenge superoxide anion with IC50 value of 27.72 micrograms/ml compared to Trolox (IC50 value = 22.08 micrograms/ml) and ascorbic acid (IC50 value = 14.65 micrograms/ml). The hydroxyl radical scavenging activity of sulfinpyrazone is in a concentration-dependent fashion. In the range of concentrations used, sulfinpyrazone was not a scavenger toward H2O2. However, the intracellular H2O2-induced 2',7'-dichlorofluorescin diacetate (DCF-DA) fluorescence in HL-60 cells was significantly reduced by sulfinpyrazone during 30-60 min of incubation. Finally, phorbol-12-myristate-13-acetate induced-lucigenin chemiluminescence in whole blood was markedly inhibited by sulfinpyrazone. Our results suggest a new direction for the pharmacological actions of sulfinpyrazone in free radical scavenging properties.
Yang
Mei-Ling
ML
Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, No. 1, Jen-Ai Road, Sec. 1, Taipei, Taiwan, ROC.
Huang
Tien-Shang
TS
Lee
Yashang
Y
Lu
Fung-Jou
FJ
D000166:Acridines / Q000378:metabolism; D001713:Biphenyl Compounds; D001769:Blood / Q000187:drug effects / Q000378:metabolism; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D018929:Cell Culture Techniques; D002474:Cell-Free System; D002839:Chromans / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005452:Fluoresceins / Q000737:chemistry / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D008163:Luminescent Measurements; D010851:Picrates / Q000378:metabolism; D013442:Sulfinpyrazone / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000187:drug effects / Q000378:metabolism
D000166:Acridines; D001713:Biphenyl Compounds; D002839:Chromans; D005452:Fluoresceins; D016166:Free Radical Scavengers; D010851:Picrates; D002084:Butylated Hydroxytoluene; C029569:diacetyldichlorofluorescein; C033472:10,10'-dimethyl-9,9'-biacridinium; C004931:1,1-diphenyl-2-picrylhydrazyl; D014969:Xanthine Oxidase; D013755:Tetradecanoylphorbol Acetate; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; D013442:Sulfinpyrazone