Evaluation of an in-house-developed radioassay kit for antibody detection in cases of pulmonary tuberculosis and tuberculous meningitis.
A radioassay for the detection of antitubercular antibody has been developed. The technique involves the addition of (125)I-labeled Mycobacterium tuberculosis antigen as a tracer, diluted clinical sample (serum or cerebrospinal fluid [CSF]), and heat-inactivated Staphylococcus aureus to capture the antibody, incubation for 4 h, and quantitation of the amount of antibody present in the sample. A total of 330 serum samples from patients with pulmonary tuberculosis and 138 control serum samples from individuals who were vaccinated with M. bovis BCG and from patients with pulmonary disorders of nontubercular origin were analyzed. Also, 26 CSF samples from patients with tuberculous meningitis and 24 CSF samples as controls from patients with central nervous system disorders of nontuberculous origin were analyzed. Sensitivities of 80 and 73% were observed for patients with pulmonary tuberculosis and tuberculous meningitis, respectively, and specificities of 90 and 88% were seen for the two groups of patients, respectively. The sensitivity was lower, however, for human immunodeficiency virus-infected patients coinfected with M. tuberculosis. The control population could be differentiated from the patient population. This assay is rapid and user friendly and, with its good sensitivity and specificity, should benefit the population by providing diagnoses early in the course of disease and, hence, permit the early administration of appropriate chemotherapy.
Laboratory Nuclear Medicine Section, Bhabha Atomic Research Centre, c/o Tata Memorial Centre Annexe. Department of Microbiology. Department of Pediatrics, K.E.M. Hospital, Mumbai-400012, India.
M
M
Kameswaran
K
K
Shetty
M K
MK
Ray
M A
MA
Jaleel
G V
GV
Kadival
D000907:Antibodies, Bacterial / Q000032:analysis*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011869:Radioligand Assay / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012698:Serologic Tests / Q000379:methods; D014390:Tuberculosis, Meningeal / Q000175:diagnosis*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D011933:Reagent Kits, Diagnostic