[Detection of the HBV DNA with Sybr green 1 realtime polymerase chain reaction].
BACKGROUND
To search a novel sensitive, specific and lower cost method applicable for quantitative analysis of the hepatitis B virus DNA extensively.


METHODS
Quantitative analysis of the DNA from 100 sera by real-time PCR with Sybr green 1. The results of Sybr's assay were compared with the results obtained with Taqman's fluorescent quantitative assay.


RESULTS
Taqman real-time PCR could help evaluate the level of virus reliably. The results of Sybr's assay were in agreement with the Taqman's assay, but detection rate was lower.


CONCLUSIONS
Sybr green 1 real-time PCR appeared to be convenient and cheap, but detection rate was lower.
Department of Infectious Diseases, First Hospital of Beijing University, Beijing 100034, China.
Yan
Y
Wang
Xiaoyuan
X
Xu
Wei
W
He
Zhihong
Z
Liu
Weibo
W
Gong
Qinhuan
Q
Wang
D052160:Benzothiazoles; D004279:DNA, Viral / Q000097:blood*; D003959:Diamines; D005456:Fluorescent Dyes; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines; D012680:Sensitivity and Specificity
D052160:Benzothiazoles; D004279:DNA, Viral; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I