Cytogenetic characterization of telomeres in the holocentric chromosomes of the lepidopteran Mamestra brassicae.
Telomeres of the Mamestra brassica holocentric chromosomes were studied by Southern blotting, in-situ hybridization and Bal31 assay evidencing the presence of the telomeric (TTAGG)n repeat. Successively, molecular analysis of telomeres showed that TRAS1 transposable elements were present at the subtelomeric regions of autosomes but not in the NOR-bearing telomeres of the Z and W sex chromosomes. TRAS1 appeared to be transcriptionally active and non-methylated, as evaluated by RT-PCR and digestion with MspI and HpaII. Finally, dot-blotting experiments showed that the 2.8 +/- 0.5% of the M. brassicae genome consists of TRAS1.
Dipartimento di Biologia Animale, Università di Modena e Reggio Emilia, Via Campi 213/D, 41100 Modena, Italy. mandriol@unimo.it
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D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D017931:DNA Primers; D009036:Moths / Q000235:genetics*; D010675:Pheromones / Q000235:genetics; D016133:Polymerase Chain Reaction; D016615:Telomere / Q000235:genetics* / Q000648:ultrastructure
D017931:DNA Primers; D010675:Pheromones