Egg transport in the coelom of the newt cynops pyrrhogaster. I. The ovulated egg is transported to the ostium by the ciliary movement of coelomic epithelial cells.
We investigated the mechanism of egg transport in the newt not only by inserting various conditioned eggs into the recipient's body but also by placing them on the coelomic epithelia of the opened body cavity in the adult female newt. Most of the inserted coelomic eggs were oviposited, while 4 of 14 inserted de-jellied uterine eggs and 3 of 10 inserted de-jellied fertilized eggs were oviposited. The coelomic eggs placed on the coelomic epithelia were transported toward the ostium and entered the ostium. The de-jellied uterine eggs and the de-jellied fertilized eggs were transported to the ostium as well. Of all the eggs examined, the coelomic egg was transported the fastest. The transport speeds of coelomic eggs treated with periodic acid and the speed of boiled coelomic eggs were less than those of untreated coelomic eggs. In contrast, the transport speeds of coelomic eggs treated with trypsin and the speed of coelomic eggs removed from their vitelline envelopes (naked eggs) were faster than those of untreated coelomic eggs. Other experiments were carried out in order to ascertain the dependence of sexual activity on egg transport. The speed of coelomic egg transport in artificially sexually activated females was faster than in sexually inactive females, although the ciliary movement could always be observed in both sexually active females and sexually inactive females. This suggests that the speed of egg transport on the coelomic epithelia is controlled by the sexual activity of the female.
Yamahama
Yumi
Y
Department of Biology, Faculty of Science, Yamagata University, Japan.
Onitake
Kazuo
K
D000818:Animals; D002465:Cell Movement; D002923:Cilia / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D008099:Liver / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010058:Oviposition; D010060:Ovulation / Q000502:physiology*; D010063:Ovum / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D012455:Salamandridae / Q000033:anatomy & histology / Q000502:physiology*