Neuropeptide F and its expression in the yellow fever mosquito, Aedes aegypti.
A neuropeptide F (NPF) was isolated from an extract of adult Aedes aegypti mosquitoes based on its immunoreactivity in a radioimmunoassay for Drosophila NPF. After sequencing the peptide, cDNAs encoding the NPF were identified from head and midgut. These cDNAs encode a prepropeptide containing a 36 amino acid peptide with an amidated carboxyl terminus, and its sequence shows it to be a member of the neuropeptide F/Y superfamily. Immunocytochemistry and Northern blots confirmed that both the brain and midgut of females are likely sources of NPF, found at its highest hemolymph titer before and 24 h after a blood meal.
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
Dawn M
DM
Stanek
Jan
J
Pohl
Joe W
JW
Crim
Mark R
MR
Brown
D000330:Aedes / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D017124:Conserved Sequence / Q000235:genetics; D004199:Disease Vectors; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D015004:Yellow Fever / Q000635:transmission
D009479:Neuropeptides; C076101:neuropeptide F