Microdissection of histologic sections: past, present, and future.
Histologic and cytologic changes are central to the diagnosis and classification of many disease processes, particularly neoplasms. The correlation of these changes with genomics, proteomics, and molecular pathways entails refined microdissection techniques that are frequently used to procure a pure population of cells from complex tissue. Here we review the past, present, and future of some of these new advances in microdissection techniques including manual techniques, laser microdissection, laser capture microdissection, and laser catapulting.
Departments of Pathology, Cell Biology and Surgery, and the UAB Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, Alabama 35233, USA.
Isam A
IA
Eltoum
Gene P
GP
Siegal
Andra R
AR
Frost
D004210:Dissection / Q000295:instrumentation / Q000379:methods*; D023281:Genomics; D006652:Histological Techniques / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007834:Lasers; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D040901:Proteomics