Higher radical scavenging activities of polyphenolic antioxidants can be ascribed to chemical reactions following their oxidation.
Radical scavenging activities of 34 natural antioxidants were investigated from an electrochemical viewpoint. While the correlation of the oxidation potentials with their DPPH radical scavenging activities (represented by EC(50)) was not high (the correlation coefficient, r=0.73), the number of electrons n required for oxidation of an antioxidant, being obtained by continuous flow-column electrolysis with a slower flow rate (0.05 ml min(-1)), did show a good correlation with EC(50) (1/EC(50)=1.67n+0.50 with r=0.94). The n values of most polyphenols were increased with a decrease in the flow rate, while those of nonpolyphenols were invariant. This suggests that a slower subsequent chemical reaction(s) should be involved in the oxidation of polyphenols, whose higher radical scavenging activities seem to be ascribed to the chemical reactions. In this study, we have proposed a possible mechanism for the oxidation of polyphenols, in which the oxidizable -OH moieties are reproduced through an oxidative dimerization (or more highly polymerization).
Hotta
Hiroki
H
Department of Chemistry, Faculty of Science, Kobe University, Nada, Japan.
Nagano
Satomi
S
Ueda
Masashi
M
Tsujino
Yoshio
Y
Koyama
Junko
J
Osakai
Toshiyuki
T
D000975:Antioxidants / Q000737:chemistry*; D001713:Biphenyl Compounds; D019281:Dimerization; D004572:Electrolysis / Q000379:methods; D005419:Flavonoids; D016166:Free Radical Scavengers / Q000737:chemistry*; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D059808:Polyphenols; D012212:Rheology
D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D011108:Polymers; D059808:Polyphenols; C004931:1,1-diphenyl-2-picrylhydrazyl