[Effect of insulin-like growth factor-I on cultured articular chondrocytes of rabbits].
OBJECTIVE
To investigate the influence of insulin-like growth factor-I (IGF-I) on biological characteristics of articular chondrocytes cultured in vitro of rabbits.


METHODS
Monolayer articular chondrocytes of 4-week old rabbits were cultured in medium with IGF-I, at the concentrations of 3, 10, 30, 100, and 300 ng/ml. The DNA content in cells and glucuronic acid content in matrix were detected on the 2nd, 4th, 6th days after culture.


RESULTS
The DNA content in cells and the glucuronic acid content in matrix in articular chondrocytes cultured in medium with IGF-I at concentrations of 3-300 ng/ml were all significantly higher than those in control group (P < 0.01), which reached the peak at the concentrations of 30-100 mg/ml on the 4th day.


CONCLUSIONS
IGF-I could obviously promote the proliferation of articular chondrocytes in vitro, and there exist time-dependent and dose-dependent effect.
Department of Orthopedic Surgery, Xijing Hospital, 4th Military Medical University, Xi'an Shaanxi, P. R. China 710032.
Gang
G
Liu
Yun-yu
YY
Hu
Ping
P
Ma
Yi-sheng
YS
Han
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D020723:Glucuronic Acid / Q000096:biosynthesis; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D011817:Rabbits
D007334:Insulin-Like Growth Factor I; D020723:Glucuronic Acid; D004247:DNA