Direct PCR detection of Burkholderia cepacia complex and identification of its genomovars by using sputum as source of DNA.
We developed a nested PCR assay that detects the recA gene of the Burkholderia cepacia complex in sputum. The product of the first PCR round is also used to identify the genomovar of the pathogen. The protocol achieves high sensitivity and specificity with simple interpretation of genomovar status.
Drevínek
Pavel
P
2nd Department of Pediatrics, 2nd Medical School of Charles University, Prague, Czech Republic. pavel.drevinek@Lfmotol.cuni.cz
Hrbácková
Hana
H
Cinek
Ondrej
O
Bartosová
Jana
J
Nyc
Otakar
O
Nemec
Alexandr
A
Pohunek
Petr
P
D000293:Adolescent; D000328:Adult; D019121:Burkholderia Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D016956:Burkholderia cepacia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000453:epidemiology / Q000382:microbiology*; D018153:Czech Republic / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011938:Rec A Recombinases / Q000235:genetics; D012680:Sensitivity and Specificity; D013183:Sputum / Q000737:chemistry*
D004269:DNA, Bacterial; D011938:Rec A Recombinases