[Approach to application of laser scanning confocal microscope in bone morphometry].
The laser scanning confocal microscope(LSCM) is the new high distinguishing microscope, which can be used in observation on fluorescence, since 1990. The bone morphometry is an important way to study metabolic bone diseases. Our study on research combination of LSCM with bone morphometry in observing microarchitecture of bone found that LSCM could make the photo of bone trabecula distinctly and its location accurately. Because of its technical advantages, LSCM could link histochemical or immunohistochemical method for the bone morphometry and bone cells at the same time and obserrate the thick-slide. It is a new method and way to study and diagnose metabolic bone diseases.
Institute of Metabolism and Endocrinology, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
E Y
EY
Liao
C
C
Yang
R C
RC
Dai
W B
WB
Wang
X G
XG
Deng
D000818:Animals; D005260:Female; D018613:Microscopy, Confocal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013131:Spine / Q000473:pathology; D013977:Tibia / Q000473:pathology*