Probing the effect of a 3'-S-phosphorothiolate link on the conformation of a DNA:RNA hybrid; implications for antisense drug design.
The effects of a single 3'-S-phosphorothiolate link in the DNA strand of a DNA:RNA dodecamer duplex is described; the sulfur induces a conformational shift in the (attached) sugar pucker, as shown by 1H NMR studies, and increases the thermal stability of the duplex compared to the non-modified system.
Department of Chemistry, University of Leeds, Leeds, UK LS2 9JT.
Andrew P G
AP
Beevers
Kevin J
KJ
Fettes
Ian A
IA
O'Neil
Stanley M
SM
Roberts
John R P
JR
Arnold
Richard
R
Cosstick
Julie
J
Fisher
D004247:DNA / Q000737:chemistry*; D015195:Drug Design; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis*; D010710:Phosphates / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
C465503:3'-S-phosphorothiolate; D007202:Indicators and Reagents; D016376:Oligonucleotides, Antisense; D010710:Phosphates; D012313:RNA; D004247:DNA