Evaluation of antiviral activity of different origin compounds by flow cytometry.
Against many viral diseases caused for example by HSV, EBV, CMV, HIV, RSV, HCV for which vaccines are not available, chemiotherapeutics seem to have the principal significance. High progress in development of new antiviral compounds is observed. In addition to synthetic compounds a large number of naturally occurring substances have been shown to posses antiviral activity. One of such substance is tannic acid. In this study comparison of antiviral activity of tannic acid, acyclovir (ACV) and ganciclovir (GCV) against cytomegalovirus (CMV) is presented. The MRC5 cells infected with CMV and treated with different compounds were analyzed by flow cytometry and cythopatic effect inhibition test for inhibition of virus replication and by MTT assay for cytotoxity. It has been shown that tannic acid has antiviral activity against cytomegalovirus and that expression of virus antigens measured as median fluorescence intensity (MFI) by flow cytometry can be used for evaluation of virus replication.
Department of Virology, National Institute of Hygiene, Warsaw, Chocimska, Poland.
Joanna
J
Siennicka
Agnieszka
A
Trzcińska
Miroslaw
M
Kańtoch
D000212:Acyclovir / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000187:drug effects*; D003586:Cytomegalovirus Infections / Q000188:drug therapy; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D005434:Flow Cytometry; D015774:Ganciclovir / Q000494:pharmacology; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D008168:Lung / Q000166:cytology / Q000821:virology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D047348:Hydrolyzable Tannins; D015774:Ganciclovir; D000212:Acyclovir