[Pattern of formation of nuclear chromosomal apparatus after remote hybridization in wheat and buckwheat: effect of low temperature].
We studied the pattern of embryo formation in wheat and buckwheat interspecific hybrids. Formation of non-reduced gametes in the embryo sac was induced by low temperature (4-7 degrees C). Changed thermal conditions during meiosis proved to favor formation of hybrid embryos. Vital embryos have been obtained. Karyotypic analysis on the metaphase plates from the root meristem demonstrated different genotypes. Heterogeneous embryos caring both maternal and paternal chromosomes as well as the embryos caring solely maternal or paternal chromosomes have been revealed. Spermatozoa penetrate the embryo sac as a cell, which increases aminopeptidase activity. Sometimes low temperature induces formation of somatic embryos from nucellus cells in the plants.
Institute of Biochemistry and Genetics, Ufa Scientific Center, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa, 450054 Russia.
M S
MS
Iumaguzhin
R A
RA
Fatkhutdinova
D002467:Cell Nucleus / Q000235:genetics*; D002678:Chimera; D002875:Chromosomes; D003080:Cold Temperature; D019612:Fagopyrum / Q000235:genetics*; D007621:Karyotyping; D008540:Meiosis; D012639:Seeds / Q000502:physiology; D013696:Temperature; D014908:Triticum / Q000235:genetics*