Implantation of VX2 carcinoma into the liver of rabbits: a comparison of three direct-injection methods.
The efficacy of agarose in preventing VX2 carcinoma cell leakage was evaluated and the results were compared with two traditional methods. Forty-five rabbits were divided into 3 groups: Group 1, VX2 tumor cells were injected directly into the liver and no special procedure after removal of the needle; Group 2, the puncture site was gently compressed, using an alcoholic cotton gauze, for three minutes; Group 3, 0.2 ml of heated liquid agarose was injected to seal the aperture after injection of VX2 cells. The leakage rates were 80%, 53.3% and 6.6% for group 1, group 2 and group 3, respectively. We consider agarose is a useful material in preventing the leakage in the establishment of VX2 liver tumor models.
Department of Nuclear Medicine, Taichung Veterans General Hospital, 160 Taichung Harbor Road Section 3, Taichung, Korea.
Wan-Yu
WY
Lin
Jeonhor
J
Chen
Yungchang
Y
Lin
Kunwang
K
Han
D000818:Animals; D002277:Carcinoma / Q000473:pathology*; D004195:Disease Models, Animal; D006358:Hot Temperature; D007267:Injections; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D009366:Neoplasm Seeding; D009368:Neoplasm Transplantation / Q000379:methods*; D011817:Rabbits; D012685:Sepharose
D012685:Sepharose