A rapid method for measuring intracellular pH using BCECF-AM.
A rapid intracellular pH (pH(i)) measurement method based on initial rate of increase of fluorescence ratio of 2',7'-bis(2-carboxyethyl)-5,6-carboxyfluorescein upon dye addition to a cell suspension in growth medium is reported. A dye transport model that describes dye concentration and fluorescence values in intracellular and extracellular spaces provides the mathematical basis for the approach. Experimental results of ammonium chloride challenge response of the two suspension cells, Spodoptera frugiperda and Chinese hamster ovary (CHO) cells, successfully compared with results obtained using traditional perfusion method. Since the cell suspension does not require any preparation, measurement of pH(i) can be completed in about 1 min minimizing any potential errors due to dye leakage.
Ozkan
Pinar
P
Molecular Biology and Genetics Department, Golden Horn University, Istanbul, Turkey.
Mutharasan
Raj
R
D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000737:chemistry; D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002478:Cells, Cultured / Q000737:chemistry*; D006224:Cricetinae; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005822:Genetic Vectors; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D015203:Reproducibility of Results; D018411:Spodoptera
D005452:Fluoresceins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; C057433:2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester; D000469:Alkaline Phosphatase