BrdU-, neuroD (nrd)- and Hu-studies reveal unusual non-ventricular neurogenesis in the postembryonic zebrafish forebrain.
In the postembryonic zebrafish forebrain, subpial locations of neurogenesis do exist in the early cerebellar external granular layer, and--unusually among vertebrates--in the primordial pretectal (M1) and preglomerular (M2) Anlagen as shown here with 5-bromo-2'-deoxyuridine (BrdU)/Hu-immunocytochemistry and in situ hybridization of neuroD. An intermediate BrdU incubation time of 12-16 h reveals in addition to proliferative ventricularly located cells those in M1 and M2. This BrdU saturation-labeling shows--in conjunction with a Hu-assay demonstrating earliest neuronal differentiation--that proliferating cells in M1 and M2 represent neuronal progenitors. This is demonstrated by single BrdU-labeled and double BrdU-/Hu-labeled cells in these aggregates. Further, expression of NeuroD--a marker for freshly determined neuronal cells--confirms this unusual subpial postembryonic forebrain neurogenesis.
Mueller
Thomas
T
FB 2, Brain Research Institute, University of Bremen, PO Box 330440, 28344 Bremen, Germany.
Wullimann
Mario F
MF
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D001973:Bromodeoxyuridine / Q000378:metabolism*; D002454:Cell Differentiation; D002455:Cell Division; D051959:ELAV Proteins; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007814:Larva / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D008938:Mitosis; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D016548:Prosencephalon / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
D051792:Basic Helix-Loop-Helix Proteins; D001973:Bromodeoxyuridine; D051959:ELAV Proteins; D009419:Nerve Tissue Proteins; D018809:Proliferating Cell Nuclear Antigen; D016601:RNA-Binding Proteins; C093564:Neurogenic differentiation factor 1