The ferredoxin docking site of photosystem I.
The reaction center of photosystem I (PSI) reduces soluble ferredoxin on the stromal side of the photosynthetic membranes of cyanobacteria and chloroplasts. The X-ray structure of PSI from the cyanobacterium Synechococcus elongatus has been recently established at a 2.5 A resolution [Nature 411 (2001) 909]. The kinetics of ferredoxin photoreduction has been studied in recent years in many mutants of the stromal subunits PsaC, PsaD and PsaE of PSI. We discuss the ferredoxin docking site of PSI using the X-ray structure and the effects brought by the PSI mutations to the ferredoxin affinity.
Sétif
Pierre
P
CEA Saclay, Département de Biologie Joliot-Curie, Service de Bioénergétique and URA CNRS 2096, 91191 Gif sur Yvette, Cedex, France. setif@dsvidf.cea.fr
Fischer
Nicolas
N
Lagoutte
Bernard
B
Bottin
Hervé
H
Rochaix
Jean-David
JD
D000595:Amino Acid Sequence; D002736:Chloroplasts / Q000737:chemistry; D000458:Cyanobacteria / Q000737:chemistry*; D005288:Ferredoxins / Q000737:chemistry*; D008565:Membrane Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000235:genetics; D045331:Photosystem I Protein Complex; D010940:Plant Proteins / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry; D016415:Sequence Alignment
D005288:Ferredoxins; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D011506:Proteins; C070290:PsaD protein, cyanobacteria; C089909:photosystem I, psaB subunit; C079741:photosystem I, psaE subunit