Complete nucleotide sequence, genome organization and phylogenic analysis of the canine calicivirus.
The complete genomic sequence of canine calicivirus (CaCV) isolated from feces of a dog with diarrhea was determined. The CaCV genome, a positive-sense single-stranded RNA, contained 8513 nucleotides excluding the poly(A) tail and was longer than that of any other calicivirus strain with a completely known sequence. There were three open reading frames (ORF1, nt 12-5801; ORF2, nt 5805-7880; and ORF3, nt 7877-8278). ORF1 encoded a polyprotein (calculated Mr of 214,802) which had the conserved motifs of non-structural proteins of other caliciviruses and picornaviruses. Regions containing characteristic motifs in the non-structural polyprotein of CaCV showed highest similarity with those of the species Feline calicivirus and Vesicular exanthema of swine virus in the genus Vesivirus. Phylogenic analysis indicated that CaCV formed a distinct branch within the genus. Our results strongly suggested that CaCV is a new species in the genus Vesivirus.
Matsuura
Yuichi
Y
Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Japan.
Tohya
Yukinobu
Y
Nakamura
Kazuya
K
Shimojima
Masayuki
M
Roerink
Frank
F
Mochizuki
Masami
M
Takase
Kozo
K
Akashi
Hiroomi
H
Sugimura
Takaaki
T
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002139:Caliciviridae / Q000145:classification* / Q000235:genetics*; D017124:Conserved Sequence; D004279:DNA, Viral / Q000032:analysis; D004285:Dogs; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D011061:Poly A / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000235:genetics
D004279:DNA, Viral; D017361:Viral Nonstructural Proteins; D011061:Poly A