Molecular cloning and characterization of human DDX36 and mouse Ddx36 genes, new members of the DEAD/H box superfamily.
With the strategy of homologue molecular cloning using the sequence of the maleless gene (mle) of Drosophila, the novel homologous human and mouse genes with longer DNA/RNA helicase box (DEAD/DEAH box), named DDX36 and Ddx36 genes, respectively, were cloned as new members of the DEAD/H box superfamily. The predicted protein encoded by human DDX36 gene has a sequence identity of 37% and similarity of 58% with the MLE protein of Drosophila and 91% and 94% with the predicted protein encoded by mouse Ddx36 gene, respectively. Northern blotting of DDX36 shows a single strong signal of 3.8 kb in the hybridization pattern in human testis but no or very weak signal in other tissues. The DDX36 gene is mapped to chromosome 3q25.1-3q25.2, in which 26 exons and 25 introns have been identified. DDX36 and Ddx36 genes may be involved in sex development, spermatogenesis and male reproduction.
Laboratory of Human Reproductive Engineering, Central South University Xiangya Medical College, Changsha 410078, China. lgxdirector@sina.com
Jun-Jiang
JJ
Fu
Lu-Yun
LY
Li
Guang-Xiu
GX
Lu
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002868:Chromosomal Proteins, Non-Histone; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D053487:DEAD-box RNA Helicases; D004265:DNA Helicases / Q000145:classification / Q000235:genetics*; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D020365:RNA Helicases / Q000145:classification / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics
D002868:Chromosomal Proteins, Non-Histone; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D014157:Transcription Factors; C070837:mle protein, Drosophila; C466988:DHX36 protein, human; C466989:Dhx36 protein, mouse; D004265:DNA Helicases; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases