A physical map of the mouse genome.
A physical map of a genome is an essential guide for navigation, allowing the location of any gene or other landmark in the chromosomal DNA. We have constructed a physical map of the mouse genome that contains 296 contigs of overlapping bacterial clones and 16,992 unique markers. The mouse contigs were aligned to the human genome sequence on the basis of 51,486 homology matches, thus enabling use of the conserved synteny (correspondence between chromosome blocks) of the two genomes to accelerate construction of the mouse map. The map provides a framework for assembly of whole-genome shotgun sequence data, and a tile path of clones for generation of the reference sequence. Definition of the human-mouse alignment at this level of resolution enables identification of a mouse clone that corresponds to almost any position in the human genome. The human sequence may be used to facilitate construction of other mammalian genome maps using the same strategy.
The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
Simon G
SG
Gregory
Mandeep
M
Sekhon
Jacqueline
J
Schein
Shaying
S
Zhao
Kazutoyo
K
Osoegawa
Carol E
CE
Scott
Richard S
RS
Evans
Paul W
PW
Burridge
Tony V
TV
Cox
Christopher A
CA
Fox
Richard D
RD
Hutton
Ian R
IR
Mullenger
Kimbly J
KJ
Phillips
James
J
Smith
Jim
J
Stalker
Glen J
GJ
Threadgold
Ewan
E
Birney
Kristine
K
Wylie
Asif
A
Chinwalla
John
J
Wallis
LaDeana
L
Hillier
Jason
J
Carter
Tony
T
Gaige
Sara
S
Jaeger
Colin
C
Kremitzki
Dan
D
Layman
Jason
J
Maas
Rebecca
R
McGrane
Kelly
K
Mead
Rebecca
R
Walker
Steven
S
Jones
Michael
M
Smith
Jennifer
J
Asano
Ian
I
Bosdet
Susanna
S
Chan
Suganthi
S
Chittaranjan
Readman
R
Chiu
Chris
C
Fjell
Dan
D
Fuhrmann
Noreen
N
Girn
Catharine
C
Gray
Ran
R
Guin
Letticia
L
Hsiao
Martin
M
Krzywinski
Reta
R
Kutsche
Soo Sen
SS
Lee
Carrie
C
Mathewson
Candice
C
McLeavy
Steve
S
Messervier
Steven
S
Ness
Pawan
P
Pandoh
Anna-Liisa
AL
Prabhu
Parvaneh
P
Saeedi
Duane
D
Smailus
Lorraine
L
Spence
Jeff
J
Stott
Sheryl
S
Taylor
Wesley
W
Terpstra
Miranda
M
Tsai
Jill
J
Vardy
Natasja
N
Wye
George
G
Yang
Sofiya
S
Shatsman
Bola
B
Ayodeji
Keita
K
Geer
Getahun
G
Tsegaye
Alla
A
Shvartsbeyn
Elizabeth
E
Gebregeorgis
Margaret
M
Krol
Daniel
D
Russell
Larry
L
Overton
Joel A
JA
Malek
Mike
M
Holmes
Michael
M
Heaney
Jyoti
J
Shetty
Tamara
T
Feldblyum
William C
WC
Nierman
Joseph J
JJ
Catanese
Tim
T
Hubbard
Robert H
RH
Waterston
Jane
J
Rogers
Pieter J
PJ
de Jong
Claire M
CM
Fraser
Marco
M
Marra
John D
JD
McPherson
David R
DR
Bentley
D000818:Animals; D002875:Chromosomes / Q000235:genetics; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D020451:Contig Mapping / Q000379:methods; D016678:Genome; D015894:Genome, Human; D006801:Humans; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping / Q000379:methods*; D021101:Radiation Hybrid Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D026801:Synteny