[Protective effect of ischemic preconditioning on ischemia-reperfusion injury].
OBJECTIVE
To study the protective effect of ischemic preconditioning(IP) on ischemia-reperfusion injury of limb.


METHODS
One to three times of short-term ischemia was applicated before prolonged ischemia, a series of biological examinations, including creatine phosphate kinase(CPK), glutamic-oxalacetic transaminase(GOT), lactic dehydrogenase(LDH), malondiadehyde(MDA) and superoxide dismutase(SOD) were carried out in each group.


RESULTS
Compared with the control, release of skeletal muscle intracellular enzyme(CPK, GOT and LDH) decreased in experiment groups after preconditioning had been performed. The serum concentration of MDA was lower, activity of SOD was higher, and pathological lesion was less in preconditioning groups than that in non-preconditioning group. Different frequency of preconditioning led to different results.


CONCLUSIONS
Preconditioning has protective effect on ischemia-reperfusion injury of limbs, and the effect of IP is correlated with the number of preconditioning cycles.
Department of Orthopeadics, Xiangya Hospital, Hunan Medical University, Changsha 410008.
W G
WG
Wang
Z S
ZS
Deng
J L
JL
Gong
Y
Y
Zhu
D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D005260:Female; D006614:Hindlimb / Q000098:blood supply; D019194:Ischemic Preconditioning; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000097:blood
D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D003402:Creatine Kinase