Functional screening of intracellular proteins in single cells and in patterned cell arrays using electroporation.
A tool for detection and characterization of intracellular enzyme-substrate and receptor-ligand interactions inside the cytoplasm of single targeted cells or small confined groups of cells is presented. Fluorogenic enzyme substrates and receptor ligands were rapidly delivered by electroosmosis and internalized by electroporation in cells using an electrolyte-filled capillary (EFC) biased at a high voltage. Specifically, alkaline phosphatase and proteases were detected in single NG108-15 cells using fluorescein diphosphate and casein BODIPY FL, respectively. The intracellular 1,4,5-inositol triphosphate (IP3) and ryanodine receptors were detected after EFC introduction of the selective receptor agonists IP3 and cyclic adenosine diphosphate ribose (cADPr), respectively. Receptor activation in both cases resulted in increased cytosolic concentrations of free calcium ions that were measured using the calcium-ion-selective probe, fluo-3. The effect of cADPr could be blocked by coadministration of the ryanodine receptor antagonist ruthenium red. Furthermore, electroporation of a plurality of cells grown in microwell structures (100 x 100 x 45 microm) molded in PDMS is demonstrated. The methods and systems described using an EFC for electroporation and delivery of protein markers, ligands, and substrates might be useful in high-throughput screening of intracellular targets, with applications in proteomics and phenotype profiling, as well as in drug discovery.
Nolkrantz
Kerstin
K
Department of Chemistry, Göteborg University, Sweden.
Farre
Cecilia
C
Hurtig
K Johan
KJ
Rylander
Petra
P
Orwar
Owe
O
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002460:Cell Line; D018274:Electroporation / Q000379:methods*; D010450:Endopeptidases / Q000032:analysis; D004798:Enzymes / Q000032:analysis; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D008024:Ligands; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis
D004798:Enzymes; D005456:Fluorescent Dyes; D008024:Ligands; D011506:Proteins; D011956:Receptors, Cell Surface; D000469:Alkaline Phosphatase; D010450:Endopeptidases