Distribution of the hyaluronate lyase encoding gene hylB and the insertion element IS1548 in streptococci of serological group B isolated from animals and humans.
The present study was performed to investigate streptococci of serological group B obtained from various sources and group B streptococcal reference strains for serotype, hyaluronate lyase enzyme activity, the occurrence of the hylB gene and the insertion sequence IS1548. All group B streptococci were identified by cultural, biochemical, and serological properties and by polymerase chain reaction amplification of species-specific parts of the 16S-23S rDNA intergenic spacer region, the 16S rRNA gene and the CAMP-factor (cfb) gene. Of the 73 group B streptococci investigated, 59 strains displayed hyaluronate lyase enzyme activity. All hyaluronate-lyase-positive strains and three phenotypically hyaluronate-lyase-negative strains had a hylB gene with an amplicon size of 3.3kb. Eleven of the 14 phenotypically hyaluronate-lyase-negative strains generated a hylB gene PCR product with a size of 4.6kb, and 10 of these strains displayed a IS1548 amplicon with a size of 0.98kb. The hyaluronate-lyase-negative isolates were mainly observed among group B streptococci of serotype III/Rib. All strains harbouring IS1548 had an additional copy of IS1548 located downstream of the C5a peptidase (scpB) gene.
Yildirim
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AO
Institut für Frankfurter Strasse 107, 35392 der Justus-Liebig-Universität Giessen, Pharmakologie und Toxikologie, Germany.
Fink
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K
Lämmler
Ch
Ch
D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D011133:Polysaccharide-Lyases / Q000235:genetics*; D013045:Species Specificity; D013292:Streptococcus agalactiae / Q000145:classification* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification
D004251:DNA Transposable Elements; D011133:Polysaccharide-Lyases; C100002:hyaluronate lyase