[Cloning and expression of simplex herpes virus ? US4 fragment].
BACKGROUND
To get early laboratory study of type specific antigenicity of herpes simplex virus II.


METHODS
PCR and prokaryotic expression technique.


RESULTS
Herpes simplex virus II type specific gene fragment was expressed in E.coli and the products can be used as specific antigen for the detection of anti\HSV in the recovery sera.


CONCLUSIONS
Cloning and express of HSVII type specific antigen found the basis for developing specific diagnosis methods and vaccine of HSV.
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Yao
Y
Yi
Wenbo
W
Xu
Mingcheng
M
Zhang
Yongdong
Y
Zhou
Yong
Y
Li
Shengli
S
Bi
D000956:Antigens, Viral / Q000276:immunology; D003001:Cloning, Molecular; D015870:Gene Expression; D018258:Herpesvirus 2, Human / Q000235:genetics*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000276:immunology
D000956:Antigens, Viral; D007074:Immunoglobulin G; D011994:Recombinant Proteins