Expression of CD10 by stromal cells during colorectal tumor development.
CD10 is a cell surface metalloprotease expressed by a variety of normal cell types, including lymphoid precursor cells, germinal center B lymphocytes, and some epithelial cells. We noticed that stromal cells of some cancers are positive for CD10. In this study, we investigated the role of CD10 produced by the stromal cells of colorectal neoplasms in the progression of colorectal neoplasms. Immunohistochemical examination of CD10 and p53 was performed in 169 colorectal epithelial neoplasms representing various stages of carcinogenesis. The results were correlated with the morphologic characteristics of the neoplasms. There was no expression of CD10 in the stromal cells of normal colorectal tissue. CD10-positive stromal cells were present adjacent to the tumor cells in 16 of 73 adenomas with mild or moderate dysplasia. More frequent expression of CD10 by the stromal cells was detected in adenomas with severe dysplasia (12 of 17), intramucosal carcinomas (10 of 16), and invasive carcinomas (50 of 63) than in adenomas with mild or moderate dysplasia (P < 0.0001). Expression of CD10 by > 10% of the stromal cells was detected only within the area of the invasive growth front of invasive carcinomas, not in adenomas and in only 1 of the intramucosal carcinomas. The difference between invasive and non invasive tumors was significant (P < 0.0001). The stromal expression of CD10 was significantly associated with the accumulation of p53 and a larger tumor size. These results indicate that CD10 expression is an integral part of colorectal carcinogenesis. CD10 expression seems to contribute to the invasion and thus probably facilitates metastasis.
Department of Pathology, Tokyo Medical University, Tokyo, Japan.
Hitoshi
H
Ogawa
Keiichi
K
Iwaya
Miki
M
Izumi
Masahiko
M
Kuroda
Hiromi
H
Serizawa
Yasuhisa
Y
Koyanagi
Kiyoshi
K
Mukai
D000199:Actins / Q000032:analysis; D000236:Adenoma / Q000201:enzymology / Q000473:pathology; D003106:Colon / Q000201:enzymology; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000473:pathology; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D015260:Neprilysin / Q000032:analysis*; D012007:Rectum / Q000201:enzymology; D017154:Stromal Cells / Q000201:enzymology*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D000199:Actins; D016159:Tumor Suppressor Protein p53; D015260:Neprilysin