[Expression, purification and diagnostic application of EBV TK kinase].
BACKGROUND
To find a rapid and sensitive method for early diagnosis of nasopharyngeal carcinoma by using EBV TK kinase.


METHODS
Prokaryotic expression plasmid pRSETTK was constructed. EBV TK kinase was highly expressed in E.coil BL21 (DE3). The authors identified specificity of TK kinase by Western blot, then used purified TK kinase in ELISA to detect the IgG antibody in the serum of NPC patients.


RESULTS
Specific IgG antibody against TK kinase was found in the serum of NPC patients. The specificity and sensitivity of TK kinase were both 100% in Western blot and were 98.0% and 93.4% respectively in ELISA.


CONCLUSIONS
The EBV TK kinase showed high specificity and sensitivity in ELISA, therefore it can be used for early diagnosis of NPC
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Bin
B
Huang
Bojun
B
Li
Weimin
W
Zhou
Guohua
G
Pi
Weiping
W
Tang
Shuyan
S
Gu
D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004854:Herpesvirus 4, Human / Q000201:enzymology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013937:Thymidine Kinase / Q000096:biosynthesis* / Q000302:isolation & purification
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D013937:Thymidine Kinase