Characterization of copolymer hydroxybutyrate/hydroxyvalerate from saponified vernonia, soybean, and "spent" frying oils.
Poly(beta-hydroxyalkanoate)s (PHAs) were biosynthesized by Ralstonia eutropha (formerly known as Alcaligenes eutrophus) by using saponified soybean, vernonia, and "spent" frying oils. These PHAs were isolated and characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), gas chromatography/mass spectrometry (GC/MS), proton nuclear magnetic resonance spectrometry (1H NMR), and 2-dimensional homonuclear (1H-1H) correlation spectroscopy (COSY). The analytical results revealed that the PHAs produced from saponified vernonia and soybean oils were copolymers of hydroxybutyrate (HB) and hydroxyvalerate (HV), that is, P(HB/HV)s, whereas the saponified "spent" frying oil produced only poly(beta- hydroxybutyrate) (PHB) homopolymer. MALDI-MS, GC/MS, and NMR independently confirmed the composition of the PHAs. Saponified soybean oil and vernonia oil PHAs contained approximately 4 and 1% HV units, respectively. For comparison, commercial PHB and P(HB/HV), produced by R. eutropha by using glucose and a cosubstrate of glucose and propionic acid, respectively, as carbon sources, were similarly characterized.
Saeed
Kamal A
KA
Department of Chemistry, Howard University, Washington, DC 20059, USA.
Eribo
Broderick E
BE
Ayorinde
Folahan O
FO
Collier
Ledelle
L
D004042:Dietary Fats, Unsaturated / Q000032:analysis*; D004951:Esterification; D005504:Food Analysis / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D009682:Magnetic Resonance Spectroscopy; D011091:Polyesters / Q000032:analysis* / Q000737:chemistry; D012503:Saponins; D013024:Soybean Oil / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D035741:Vernonia; D000094122:Polyhydroxybutyrates
D004042:Dietary Fats, Unsaturated; D011091:Polyesters; D012503:Saponins; D013024:Soybean Oil; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates