Copper/zinc superoxide dismutases in Dictyostelium discoideum: amino acid sequences and expression kinetics.
Genes for Copper/Zinc superoxide dismutases, designated sodA and sodB, in the cellular slime mold Dictyostelium discoideum were analyzed. It was found that these gene products contain charged amino acid residues and hydrophobic stretches in their N-terminal regions, suggesting that they are extracellular Cu/Zn superoxide dismutases. The sodA and sodB are expressed in cells in the growth phase and throughout the developmental phases, suggesting that they are the housekeeping genes. The sodA is induced by H(2)O(2) exposure but not by UV irradiation, whereas sodB is induced both by H(2)O(2) exposure and UV irradiation. Such distinct kinetics of the expression patterns suggests that these enzymes play unique roles in D. discoideum.
Tsuji
Atsushi
A
Division of Bioengineering, Faculty of Engineering, Toyama University, Japan.
Akaza
Yuya
Y
Kodaira
Ken-Ichi
K
Yasukawa
Hiroo
H
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D018076:DNA, Complementary / Q000378:metabolism; D004023:Dictyostelium / Q000201:enzymology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007700:Kinetics; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D017434:Protein Structure, Tertiary; D017382:Reactive Oxygen Species; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism; D014466:Ultraviolet Rays
D000596:Amino Acids; D018076:DNA, Complementary; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase