Determination of chiral ratio of o,p-DDT and o,p-DDD pesticides on polysaccharides chiral stationary phases by HPLC under reversed-phase mode.
A simple and reliable HPLC method for the chiral resolution of o,p-DDT and o,p-DDD is described. The enantiomeric resolution of o,p-DDT and o,p-DDD has been achieved on Chiralpak AD-R, Chiralcel OD-R, and Chiralcel OJ-R chiral stationary phases. The mobile phases used were acetonitrile-water (50:50 [v/v]) and acetonitrile-2-propanol (50:50 [v/v]) at a flow rate of 1.0 mL/min. For both pesticides detection was done at 220 nm. The values for o,p-DDT of alpha and R(s) varied from 1.24 to 2.52 and from 0.80 to 2.47, respectively. The values of alpha and R(s) for o,p-DDD were 1.26 and 0.60, respectively.
Pharmaceutical Analysis Laboratory, Biological and Medical Research Department (MBC-03), King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
Imran
I
Ali
Hassan Y
HY
Aboul-Enein
D018931:Antineoplastic Agents, Hormonal / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003634:DDT / Q000032:analysis* / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis; D004968:Estrogens, Non-Steroidal / Q000032:analysis* / Q000737:chemistry*; D007536:Isomerism; D008939:Mitotane / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results
D018931:Antineoplastic Agents, Hormonal; D004785:Environmental Pollutants; D004968:Estrogens, Non-Steroidal; D008939:Mitotane; D003634:DDT; C016340:o,p'-DDT