Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats.
BACKGROUND
T-cells extravasation and CNS parenchyma infiltration during autoimmune neurodegenerative disease can be evoked by local antigen presenting cells. Studying the chemoattracting potential of spinal perivascular macrophages (SPM) during experimental allergic encephalomyelitis (EAE), we observed numerous infiltrates of densely-packed mononuclear cells. Apart from the poor spatial and optical resolution, no differentiation between the resident SPM (mabs ED1+, ED2+) and the just recruited monocytes/macrophages (mab ED1+) was possible.


RESULTS
This is why we labeled SPM by injections of different fluoresecent dyes into the lateral cerebral ventricle before induction of active EAE. Within an additional experimental set EAE was induced by an intraperitoneal injection of T-cells specifically sensitized to myelin basic protein (MBP) and engineered to express the green fluorescent protein (GFP). In both experiments we observed a strong activation of SPM (mabs OX6+, SILK6+, CD40+, CD80+, CD86+) which was accompanied by a consistently increased expression of ICAM-1, VCAM-1, and the chemokines MCP-1 and MIP-1alpha.


CONCLUSIONS
These observations indicate that SPM play a role in promoting lymphocyte extravasation.
Institut für Anatomie der Universität zu Köln, Germany. nils.hofmann@gmx.net
Nils
N
Hofmann
Nina
N
Lachnit
Michael
M
Streppel
Brigitte
B
Witter
Wolfram F
WF
Neiss
Orlando
O
Guntinas-Lichius
Doychin N
DN
Angelov
D000818:Animals; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism* / Q000473:pathology; D016130:Immunophenotyping; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D008214:Lymphocytes / Q000737:chemistry; D008262:Macrophage Activation / Q000502:physiology; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis*; D008264:Macrophages / Q000378:metabolism* / Q000502:physiology; D008297:Male; D020335:Paraparesis / Q000473:pathology; D010586:Phagocytes / Q000737:chemistry; D051381:Rats; D011917:Rats, Inbred Lew; D013116:Spinal Cord / Q000098:blood supply* / Q000737:chemistry / Q000473:pathology; D013194:Staining and Labeling; D015854:Up-Regulation / Q000502:physiology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis*
D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019402:Macrophage Inflammatory Proteins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1