A comparative study of commercial liquid chromatographic detectors for the analysis of underivatized amino acids.
This study compares the main commercial detectors that can detect amino acids in their underivatized form. The detectors tested are: the chemiluminescent nitrogen detector (CLND), the evaporative light scattering detector (ELSD), the nuclear magnetic resonance spectrometer, conductivity detector, refractive index, UV, and electrospray quadrupole mass spectrometry (in simple and tandem MS mode). As ELSD, CLND and MS require a volatile mobile phase, an ion-pair reversed-phase liquid chromatographic system was selected, consisting of an octadecyl column and an aqueous mobile phase containing pentadecafluorooctanoic acid as volatile ion-pairing reagent. Underivatized taurine, hypotaurine, aspartic acid, hydroxyproline, asparagine, serine, glycine, glutamine, cysteine, glutamic acid, threonine and alanine were simultaneously analysed with each detector. In order to test the applicability of these detectors to "real world" samples, the amino acid stoichiometry of the tetrapeptide Gly-Gly-Asp-Ala was determined with each detector after acid hydrolysis. The detectors were compared in terms of linearity, limit of detection, advantages and disadvantages as well as special features (capacity to provide structural information, specificity, quantification with single calibration curve, etc.).
Petritis
Konstantinos
K
Institut de Chimie Organique et Analytique, CNRS UMR-6005, Université d'Orléans, France.
Elfakir
Claire
C
Dreux
Michel
M
D000596:Amino Acids / Q000032:analysis*; D002853:Chromatography, Liquid / Q000295:instrumentation*; D013053:Spectrophotometry / Q000295:instrumentation* / Q000379:methods
D000596:Amino Acids