PsrR, a member of the AraC family of transcriptional regulators, is required for the synthesis of Wolinella succinogenes polysulfide reductase.
Wolinella succinogenes grows by polysulfide respiration with formate or hydrogen as electron donor. Polysulfide reduction is catalyzed by the membrane-bound polysulfide reductase complex encoded by the psrABC operon. An open reading frame, designated psrR, was found in close proximity upstream of the psr operon, but oriented in the opposite direction. The deduced amino acid sequence of PsrR is similar to those of transcriptional regulators of the AraC family and includes all typical features. Polysulfide reductase is not detectable in a Delta psrR deletion mutant of W. succinogenes. Mutant cells grown with fumarate as terminal electron acceptor did not catalyze polysulfide reduction with formate or hydrogen, in contrast to the wild-type strain. The phenotype of W. succinogenes wild-type cells was restored by genomic complementation of W. succinogenes Delta psrR. The results suggest that the gene product of psrR is involved in the regulation of polysulfide reductase synthesis.
Institut für Mikrobiologie, Johann Wolfgang-Goethe Universität Frankfurt am Main, Marie-Curie-Strasse 9, 60439 Frankfurt am Main, Germany.
Stephan
S
Braatsch
Torsten
T
Krafft
Jörg
J
Simon
Roland
R
Gross
Oliver
O
Klimmek
Achim
A
Kröger
D000595:Amino Acid Sequence; D051777:AraC Transcription Factor; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D010088:Oxidoreductases / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013440:Sulfides / Q000378:metabolism; D014157:Transcription Factors; D014158:Transcription, Genetic; D016968:Wolinella / Q000201:enzymology* / Q000235:genetics
D051777:AraC Transcription Factor; D001426:Bacterial Proteins; D012097:Repressor Proteins; D013440:Sulfides; D014157:Transcription Factors; C032915:polysulfide; D010088:Oxidoreductases; C074883:polysulfide reductase