New liquid chromatography/electron ionization mass spectrometry methods in water analysis.
A method to extract and analyze organic compounds from water is presented. A solid phase micro-trap (micro-SPE) directly connected to the micro-analytical column is used. Sensibility and specificity needed for trace analysis are guaranteed by mass spectrometric electron ionization (EI) detection. A new micro-HPLC/EI-MS interface called Capillary-EI (Cap-EI) is described. The ultimate evolution of this interface is also presented: in this extremely simplified interface the analytes are nebulized, vaporized and ionized in the small volume of the ion source. This interface, called Direct-EI, exploits nano- and micro-HPLC columns with a mobile phase flow rates ranging from 0.3 to 1.5 microL/min. Contemporary use of micro SPE, Cap-EI or Direct-EI gives us a powerful technique to identify and quantify organic pollutants at part per billion level (ppb).
Cappiello
Achille
A
Istituto di Scienze Chimiche F. Bruner, Università di Urbino, P. Rinascimento 6, 61029 Urbino. achille@uniurb.it
Famiglini
Giorgio
G
Berloni
Arnaldo
A
Palma
Pierangela
P
Mangani
Filippo
F
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity; D014835:Volatilization; D014867:Water / Q000737:chemistry; D014873:Water Pollutants / Q000032:analysis*
D014873:Water Pollutants; D014867:Water