[Induction of non-disjunction of chromosome 8 by Tripterygium hypoglaucum (Lévl.) Hutch in mouse sperm].
Fluorescence in situ hybridization (FISH) was employed to detect non-disjunction of chromosome 8 during meiosis in young adult male mice after treated by Tripterygium hypoglaucum(Lévl.) Hutch(THH). The Kunming male mice were given THH(120 mg/kg, 240 mg/kg, 480 mg/kg) by abdominal cavity injection, and sacrificed 22 days sfter the treatment of THH. Epididymis sperm was used to make smear. FISH using bio-16-dUTP-labelled DNA probes specific for chromosome 8 was performed on epididymal sperm decondensed for 60 min each in 10 mmol/L DTT and 4 mmol/L LIS. The aneuploidy frequencies of chromosome 8 were significantly higher than the solvent control (P < 0.001) in both 240 mg/kg group and 480 mg/kg group. The aneuploidy frequencies were not significantly different between the sperm in 120 mg/kg group and the solvent control (P > 0.05). It is concluded that THH could induce non-disjunction of chromosome 8 in mouse sperm, and THH is a potential germinal aneugen in mammalian during the meiosis.
Wang
Xiao-Yan
XY
School of Life Sciences, Yunnan Normal University, Kunming 650092, China. jxabcdef@public.km.yn.con
Ding
Yin-Run
YR
Wang
Xu
X
D000782:Aneuploidy; D000818:Animals; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000633:toxicity*; D013094:Spermatozoa / Q000187:drug effects* / Q000648:ultrastructure; D029757:Tripterygium
D010936:Plant Extracts