Identification and initial characterization of 6,000 expressed sequenced tags (ESTs) from rat normal-growing cartilage and swarm rat chondrosarcoma cDNA libraries.
Chondrosarcoma is the second most common type of skeletal malignancy with a survival rate at five years for histological grade III of only 29 percent. The development of a reliable chondrosarcoma animal model could enable the study of tumor growth and progression, the effect of the host on tumor behavior, and the effectiveness of various therapeutic modalities. The Swarm rat chondrosarcoma is a tumor tissue line that has been maintained through the years by serial subcutaneous injections, and the histochemical characteristics of the tumor have remained essentially similar in all transplants over the years. This study was designed to initiate the characterization of the Swarm rat chondrosarcoma model by gene expression profiling as compared to normal-growing rat cartilage. Analysis of the gene expression from both libraries revealed a complex pattern of gene expression, including many genes not yet reported to be expressed by chondrocytes. It suggests that the biochemical characterization of growing cartilage and chondrosarcoma reported to date has only begun to describe the complexity of these tissues.
Morcuende
Jose A
JA
Department of Orthopaedic Surgery, University of Iowa, Iowa City 52242, USA. jose-morcuende@uiowa.edu
Huang
Xiao Dong
XD
Stevens
Jeff
J
Kucaba
Tammy A
TA
Brown
Bart
B
Abdulkawy
Hakeem
H
Scheetz
Todd E
TE
Malchenko
Sergey
S
Bonaldo
Fatima
F
Casavant
Thomas L
TL
Soares
Bento
B
D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D002813:Chondrosarcoma / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured