Chiral inversion of (R)-(-)-fenoprofen in guinea-pigs pretreated with clofibrate.
The influence of clofibrate on the stereoconversion of fenoprofen (FPF) was studied in guinea pigs. This hypolipidaemic agent has been related to some biochemical changes in the liver leading to an increase in the chiral inversion process. Two groups of animals (n = 6 per group) were pretreated with oral doses of clofibrate (280 mg/kg per day) for three days and were then given (R)- or (S)-FPF (5 mg/kg, IV). The FPF enantiomers were extracted from the guinea-pigs' plasma using a solid phase procedure and analysed by HPLC with previous derivatization with L-leucinamide. Pretreatment with clofibrate increased the chiral inversion of (R)-FPF in favour of the pharmacologically active (S)-FPF enantiomer. Before this metabolic interaction can be applied to therapy with fenoprofen, the toxic effects of (S)-(+)-FPF on the gastrointestinal and renal tracts and the interference by (R)-(-)-FPF with the metabolism of lipids should be thoroughly evaluated.
Departamento de Fisiopatología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Paraje Arroyo Seco s/n Tandil, Argentina.
M F
MF
San Martín
A
A
Soraci
F
F
Fogel
O
O
Tapia
S
S
Islas
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics*; D019540:Area Under Curve; D001711:Biotransformation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002994:Clofibrate / Q000008:administration & dosage / Q000494:pharmacology*; D005279:Fenoprofen / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics*; D006168:Guinea Pigs; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D015394:Molecular Structure; D013237:Stereoisomerism; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002994:Clofibrate; D005279:Fenoprofen