Induction of telomerase activity during an early burst of proliferation in pancreatic regeneration.
Telomerase activity (TA) and telomerase reverse transcriptase (rTERT) were investigated in Sprague-Dawley rats, killed at 6 and 12 h, and 1, 2, 3, 7, and 14 days after 90% pancreatectomy (px), by TRAPeze enzyme-linked immunosorbent assay telomerase detection and reverse transcriptase-polymerase chain reaction. TA increased at 2 days and reached a maximum at 3 days after px, when the small ductules showed the highest proliferation activity. After 3 days, TA decreased to basal levels as the ductules differentiated into new endocrine and exocrine pancreas. The expression of rTERT showed a correlation with TA. These results suggest telomerase is actively regulated during pancreatic regeneration.
Department of Pathology and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, CPO Box 8044, Seoul, South Korea.
Bong-Kyeong
BK
Oh
Je Kyung
JK
Seong
Jeung Eun
JE
Lee
Kwang Jo
KJ
Chae
Kyung Jin
KJ
Roh
Chanil
C
Park
Young Nyun
YN
Park
D000681:Amylases / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D004268:DNA-Binding Proteins; D004790:Enzyme Induction; D008297:Male; D010179:Pancreas / Q000166:cytology / Q000201:enzymology* / Q000502:physiology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000096:biosynthesis* / Q000235:genetics
D001786:Blood Glucose; D004268:DNA-Binding Proteins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D019098:Telomerase; D000681:Amylases