Granulocyte-macrophage colony-stimulating factor induces an expression program in neonatal microglia that primes them for antigen presentation.
Neonatal microglial cells respond to GM-CSF and M-CSF by acquiring different morphologies and phenotypes. To investigate the extent and consequences of this process, a global gene expression analysis was performed, with significant changes in transcript levels confirmed by biochemical analyses. Primary murine microglial cells underwent substantial expression reprogramming after treatment with GM-CSF or M-CSF with many differentially expressed transcripts important in innate and adaptive immunity. In particular, many gene products involved in Ag presentation were induced by GM-CSF, but not M-CSF, thus potentially priming relatively quiescent microglia cells for Ag presentation. This function of GM-CSF is distinct from its primary function in cell proliferation and survival.
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.
Fabio
F
Re
Svetlana L
SL
Belyanskaya
Richiard J
RJ
Riese
Barbara
B
Cipriani
Falko R
FR
Fischer
Francesca
F
Granucci
Paola
P
Ricciardi-Castagnoli
Celia
C
Brosnan
Lawrence J
LJ
Stern
Jack L
JL
Strominger
Laura
L
Santambrogio
D000818:Animals; D000831:Animals, Newborn / Q000235:genetics* / Q000276:immunology*; D017951:Antigen Presentation / Q000235:genetics*; D015415:Biomarkers / Q000032:analysis; D002403:Cathepsins / Q000096:biosynthesis / Q000235:genetics; D002478:Cells, Cultured; D018925:Chemokines / Q000096:biosynthesis / Q000235:genetics; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology*; D016173:Macrophage Colony-Stimulating Factor / Q000502:physiology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D017628:Microglia / Q000276:immunology* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010587:Phagocytosis / Q000235:genetics; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000235:genetics; D018121:Receptors, Cytokine / Q000096:biosynthesis / Q000235:genetics; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics
D015415:Biomarkers; D018925:Chemokines; D016207:Cytokines; C092228:Marco protein, mouse; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D002403:Cathepsins; D020782:Matrix Metalloproteinases