Simultaneous detection of hippuric acid and methylhippuric acid in urine by Empore disk and gas chromatography-mass spectrometry.
A method is described for the determination of hippuric acid (HA) and o-, m-, and p-methylhippuric acids (o-, m-, p-MHAs) in urine using solid-phase extraction and gas chromatography-mass spectrometry (GC-MS). The extraction procedure uses an Empore disk, derivatized into the respective trimethyl silyl derivatives. All metabolites including the internal standard (I.S.) were clearly able to be analyzed by the DB-17 column. The calibration curves for the four acids show linearity in the range of 5-70 microg/ml. The detection limit of each acid was 1.0-2.5 microg/ml.
Department of Forensic Medicine, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan. saito@is.icc.u-tokai.ac.jp
Takeshi
T
Saito
Sanae
S
Takeichi
D000328:Adult; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods / Q000706:statistics & numerical data; D006626:Hippurates / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D006801:Humans; D008297:Male; D011137:Polystyrenes; D019966:Substance-Related Disorders / Q000652:urine; D014050:Toluene / Q000009:adverse effects / Q000652:urine
D006626:Hippurates; D011137:Polystyrenes; D014050:Toluene; C003771:Amberlite XAD-2 resin; C030514:hippuric acid