[Determination of tetrandrine in human plasma by high-performance liquid chromatography].
The paper reports the determination of tetrandrine by high performance liquid chromatography (HPLC). A C18 column was used and the mobile phase was methanol-water(80:20) containing 0.03% triethylamine, detective wave was at 282 nm, the internal standard was diazepam, the sample was extracted with ether. The linear range was from 0.289 microgram.ml-1 to 4.618 micrograms.ml-1, the average recovery rate was 95.8%. The coefficients of variation of within-day and day-to-day were less than 5%. This method is simple, rapid and accurate. It can be used in biomedical sample analysis of tetrandrine.
Department of Pharmacy, Xiangya Hospital, Hunan Medical University, Changsha 410008.
G S
GS
Tan
Z Y
ZY
Dai
P S
PS
Xu
D000470:Alkaloids / Q000097:blood*; D044182:Benzylisoquinolines; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D006801:Humans
D000470:Alkaloids; D044182:Benzylisoquinolines; D004365:Drugs, Chinese Herbal; C009438:tetrandrine