Heat-shock protein (HSP70) response in the eastern oyster, Crassostrea virginica, exposed to PAHs sorbed to suspended artificial clay particles and to suspended field contaminated sediments.
Sediments are a potentially significant source of pollutants, containing not only organic contaminants but heavy metals as well. The heat shock protein response (HSP70 family) in the eastern oyster exposed to suspended clay particles spiked with polynuclear aromatic hydrocarbons (PAHs) and to suspended field contaminated sediments (SFCS) was investigated. In experiment 1, oysters were exposed to 1.0, 1.5 or 2.0 g suspended clay particles with concentrations of 65.6, 159.0 and 242 micro g PAHs per g of wet clay particles, respectively, and sampled after 40 days. Controls were exposed to 0, 1.0, 1.5, and 2.0 g suspended unspiked clay particles. In experiment 2, oysters were exposed to 0, 1.0, 1.5, and 2.0 g SFCS and the HSP70 expression was determined after 5, 10, 20 and 40 days exposure. Oysters exposed to suspended clay particles spiked with PAHs showed a significant increase in HSP70 levels, while oysters exposed to 1.0, 1.5 or 2.0 g suspended unspiked clay particles did not show changes (P=0.78) in HSP70 levels compared to the group receiving 0 g clay particles. Exposure to the SFCS resulted in a significant increase in HSP70 as a function of exposure (P<0.001) and treatment (P=0.006). The response, however, was not dose dependent. Compared to the control group (0 g SFCS), groups exposed to 1.0, 1.5 and 2.0 g SFCS reached significantly higher levels in HSP70 at 40 days of exposure, with those exposed to 2.0 g SFCS expressing the highest levels. The HSP70 expression for each treatment showed fluctuations at various time intervals. No mortalities were recorded during the exposure experiments. The major contaminants in the SFCS were PAHs, heavy metals and polychlorinated biphenyls (PCBs). These results reveal that exposure to PAHs sorbed to clay particles and to SFCS induced a HSP70 response in the eastern oyster.
Cruz-Rodríguez
Luis A
LA
Virginia Institute of Marine Science, School of Marine Science, College of William and Mary, Department of Environmental Sciences, Box 1346, Gloucester Point, VA 23062-1346, USA.
Chu
Fu-Lin E
FL
D000538:Aluminum Silicates; D000818:Animals; D015153:Blotting, Western; D000077215:Clay; D004781:Environmental Exposure / Q000009:adverse effects; D019015:Geologic Sediments / Q000737:chemistry*; D005880:Gills / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D010125:Ostreidae / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000009:adverse effects / Q000378:metabolism
D000538:Aluminum Silicates; D018840:HSP70 Heat-Shock Proteins; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D000077215:Clay