[Search for non-relative donor by the Russian register of bone marrow donors].
OBJECTIVE
To select maximally HLA compatible donor for hematological patients who need transplantation of bone marrow from non-relative donor.


METHODS
75 patients with hematological malignancy were observed. All of them have indications to non-relative transplantation of the bone marrow. Methods of polymerase chain reaction with sequence-specific primers and classic microlymphocytotoxic test were used.


RESULTS
Typing of HLA antigens of class I and alleles of class II loci enabled search for non-relative donor for transplantation of bone marrow in accordance with the requirements of the European Federation of Immunogenetics. Most of the patients (86.6%) had at least one potential HLA-A, -B, -DR compatible donor. Half of the patients had potential donors typed at the allele level by class II loci. This diminishes time of HLA compatible donor selection.


CONCLUSIONS
DNA typing enables the search for the non-relative donors meeting modern requirements. This allowed 5 non-relative bone marrow transplantations.
Zaretskaia
Iu M
IuM
Khamaganova
E G
EG
Aleshchenko
S M
SM
Murashova
L A
LA
D014408:Biomarkers, Tumor / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D005260:Female; D006680:HLA Antigens / Q000097:blood*; D006684:HLA-DR Antigens / Q000097:blood / Q000235:genetics; D059811:HLA-DRB1 Chains; D019337:Hematologic Neoplasms / Q000628:therapy*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012042:Registries; D014019:Tissue Donors; D014184:Transplantation, Homologous
D014408:Biomarkers, Tumor; D006680:HLA Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains