Induction of DNA double strand breaks in scid cells by carbon ions.
The DNA double strand breaks (DSBs) induced by X ray and carbon ion beam irradiation in scid cells were analysed using pulsed-field gel electrophoresis. Scid cells and hybrid cells were ideal to study the DNA DSB repair mechanisms, because their genetic backgrounds were identical except DNA-PK activity. Induction of DNA DSBs was determined after exposure to X rays and carbon beams. DNA DSB repair was by biphasic kinetics with a fast and a slow component. For scid cells only a slow component was observed, whereas the kinetics of DSBs repair was biphasic with a fast and a slow component. It was concluded from the experimental data that the induced DSB rejoining in scid cells was due to the lack of DNA-PK activity.
Research Centre for Isotope Science, Kumamoto University, 2-2-1 Honjo, 860-0811 Kumamoto, Japan. tshima@kaiju.medic.kumamoto-u.ac.jp
T
T
Shimasaki
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M
Ihara
Y
Y
Furusawa
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Y
Okumura
D002250:Carbon Radioisotopes; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002898:Chromosomes, Human, Pair 8 / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D016511:Severe Combined Immunodeficiency / Q000235:genetics* / Q000473:pathology; D014965:X-Rays
D002250:Carbon Radioisotopes; D004247:DNA