Immunocytochemically defined populations of neurons progressively increase in size through embryogenesis of Hydra vulgaris.
The hydra nervous system shares many features with nervous systems of more complex organisms but serves as a unique model system due to its simplicity and constant regeneration. Development of neuron populations during and after hydra embryogenesis is not well understood. In this study, neurons were identified at prehatching and posthatching stages with RFamide or JD1 antisera. These populations were further subdivided into ganglion, sensory, or unclassifiable neurons, and all identified populations were statistically analyzed over developmental time. RFamide-positive neurons appeared 20 days after the cuticle formed around the embryo. The JD1-positive neuron population appeared just after hatching, but by adulthood it had surpassed the size of the RFamide-positive population. All neuron populations progressively increased through their adult levels. Density of most of the populations, however, did not. For instance, during the 5-fold increase in size that the hydra experienced between 5 days posthatching and adulthood, the number of RFamide-positive neurons rose approximately 2-fold and the number of JD1-positive neurons 4-fold. However, the density of neurons in each of these populations fell. These data do not support the hypothesis that large-scale culling of neurons during development, frequently found in other animals, occurs in hydra.
Brumwell
Gordon B
GB
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556, USA. gordon.b.brumwell.1@nd.edu
Martin
Vicki J
VJ
D000818:Animals; D001824:Body Constitution / Q000502:physiology; D048429:Cell Size; D004625:Embryo, Nonmammalian / Q000166:cytology*; D006829:Hydra / Q000033:anatomy & histology* / Q000196:embryology*; D009474:Neurons / Q000145:classification / Q000166:cytology* / Q000502:physiology