In vitro and in vivo tests of hydrothermally synthesised hydroxyapatite coating.
High pure and crystalline Hydroxyapatite (HA) coatings on titanium alloy were prepared by hydrothermal synthesis (HS) of plasma-sprayed (PS) precursors from brushite powders (HS-HA). In vitro and in vivo tests were done to evaluate its biological property. The HS-HA coating was compared with the current PS-HA coating. Cultures of the primary osteoblasts on these two HA coatings showed similar cell attachment, proliferation and alkaline phosphatase (ALP) expression. The cell morphology on the coatings was demonstrated by scanning electron microscopy (SEM). The cell spread well at 1 day after seeding culture and the extracellular matrix was secreted after 14 days culture. Histomorphometric analysis was conducted on samples implanted in femoral bone of four dogs for 1 and 3 months, and bone-implant contact percentage was evaluated by light microscopy. The calcium and phosphate distribution on the interface of bone-implant was analysed by SEM and electron dispersive X-ray (EDX) analysis. The results show the osteoconduction of HS-HA coated implants.
Zhang
Yumei
Y
School of Materials Science and Engineering, Xi'an Jiaotong University, Xi'an 710049 People's Republic of China. grb@univ-lille2.fr
Fu
Tao
T
Han
Yong
Y
Wang
Qintao
Q
Zhao
Yimin
Y
Xu
Kewei
K
D000497:Alloys; D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry; D002452:Cell Count; D002465:Cell Movement; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D004285:Dogs; D017886:Durapatite / Q000737:chemistry*; D005269:Femur / Q000166:cytology* / Q000503:physiopathology / Q000601:surgery / Q000648:ultrastructure; D008422:Materials Testing / Q000379:methods; D016348:Osseointegration / Q000187:drug effects; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature; D014025:Titanium; D014867:Water / Q000737:chemistry
D000497:Alloys; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D014867:Water; C031462:titanium alloy (TiAl6V4); D017886:Durapatite; D014025:Titanium; C494366:calcium phosphate, dibasic, dihydrate