Michaelis-Menten elimination kinetics of acetaldehyde during ethanol oxidation.
BACKGROUND
Acetaldehyde (AcH) is a toxic metabolite of ethanol (EtOH). The pharmacokinetics of blood AcH during EtOH oxidation was studied with or without the administration of aldehyde dehydrogenase 2 inhibitor (cyanamide) in rabbits.


METHODS
An bolus of EtOH saline solution (0.25, 0.5, 1.0, 1.5, and 2.0 g/kg) was injected intravenously. Cyanamide was administered intraperitoneally (25 mg/kg body weight) to the cyanamide-treated group. Blood EtOH and AcH concentrations were measured by using head-space gas chromatography.


RESULTS
In the control group, the first peak of the blood AcH appeared immediately and the second elevation appeared 1 to 4 hr after administration at a high EtOH dose. The blood AcH levels other than the second elevation part were significantly correlated to the blood EtOH levels. In the cyanamide-treated group, a peak and a plateau formed at the time corresponding to the second peak in the control group. The peak and plateau concentration of AcH increased markedly. We attempted simultaneous curve fitting, using the five blood EtOH and AcH concentration-time curves, to determine the pharmacokinetic model. Consequently, the AcH elimination was best described by a Michaelis-Menten kinetic model in both groups.


CONCLUSIONS
The blood AcH profile was suggested to consist of the first and second components that are related to the blood EtOH concentration itself and the metabolic formation of AcH, respectively. With higher EtOH doses or aldehyde dehydrogenase 2 inhibition, the second component becomes prominent as a result of the capacity-limited property of the metabolism of AcH, which is described by Michaelis-Menten elimination kinetics.
Department of Legal Medicine, Yamaguchi University of Medicine, Minamikogushi, Ube, Japan. fujimiya@po.cc.yamaguchi-u.ac.jp
Tatsuya
T
Fujimiya
Kiyoshi
K
Yamaoka
Yumiko
Y
Ohbora
Toshihiko
T
Aki
Hiromi
H
Shinagawa
D000079:Acetaldehyde / Q000493:pharmacokinetics; D000444:Aldehyde Dehydrogenase / Q000037:antagonists & inhibitors / Q000502:physiology; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D000818:Animals; D003484:Cyanamide / Q000494:pharmacology; D000431:Ethanol / Q000493:pharmacokinetics*; D008657:Metabolic Clearance Rate / Q000502:physiology; D010084:Oxidation-Reduction; D011817:Rabbits
D000431:Ethanol; D003484:Cyanamide; C096127:ALDH2 protein, human; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D000079:Acetaldehyde