[RNA transduction by phage MS2 noninfectious virions].
Two fragments (1.9 and 1.8 kb) including, respectively, "structural" genes (A, B, and lysis polypeptide) and "replicase" gene, were obtained by reverse transcription PCR on the template of bacteriophage MS2 genome RNA. The fragments contained a common sequence which allowed the assembly of the entire functional genome sDNA. 1.8 kb fragment was subjected to two independent modifications: 1) so that the replicase gene could be replaced by other sequences no more than 1.5 kb in size and 2) it was inserted (on an artifician Tn-like structure) into E. coli chromosome. A system for replication of MS2-like corpuscles was thus created, containing a "shortened" genome and having extra genes. For extra genes, aminoglycoside-3'-phosphotransferase (kanamycin resistance) or a sequence of antiviral genetic program directed against hepatitis C virus were used.
Kniazhev
V A
VA
Institute of Physicochemical Medicine, Moscow.
Sivov
I G
IG
Sergienko
V I
VI
D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000235:genetics; D006997:Hypochlorous Acid / Q000494:pharmacology; D017909:Levivirus / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D012367:RNA, Viral; D014161:Transduction, Genetic; D014771:Virion / Q000235:genetics* / Q000472:pathogenicity
D004251:DNA Transposable Elements; D004279:DNA, Viral; D012367:RNA, Viral; D006997:Hypochlorous Acid