Localisation of CD25+ cells and MHCII+ cells in lymph nodes draining Mycobacterium avium subsp. paratuberculosis vaccination granuloma and the presence of a systemic immune response.
Vaccination of goat kids against paratuberculosis protects against lesions and clinical disease. The systemic cellular response was studied in goat kids 3-9 weeks after vaccination. Peripheral blood cells showed increased interferon-gamma production and expression of interleukin-2 receptor (CD25) after stimulation with Mycobacterium avium subsp. paratuberculosis antigens. The lymph node draining the vaccination granuloma was studied three weeks after vaccination in a parallel group of goat kids. In deep cortex, MHCII+ cells were observed surrounded by CD4+ T-cells, while follicular hypertrophy and hyperplasia were prominent in the subcapsular region and along connective tissue trabecula. Comparison of the local and systemic immune responses revealed an inverse relationship between CD25+ T-cells in the lymph node deep cortex and cells in peripheral blood that up-regulate CD25 upon in vitro stimulation, suggesting that activated and regulatory T-cells in the local lymph node influence the level of circulating antigen-specific T-cells following vaccination against paratuberculosis in goats.
Department of Morphology, Genetics and Aquatic Biology, Norwegian School of Veterinary Science, P.O. Box 8146 Dep. N-0033 Oslo, Norway. mette.valheim@vetinst.no
M
M
Valheim
H J
HJ
Hasvold
A K
AK
Storset
H J S
HJ
Larsen
C McL
CM
Press
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D015511:Goat Diseases / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control; D006041:Goats / Q000276:immunology* / Q000382:microbiology; D006099:Granuloma / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D008297:Male; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology* / Q000302:isolation & purification; D010283:Paratuberculosis / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control; D015375:Receptors, Interleukin-2 / Q000032:analysis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D000949:Histocompatibility Antigens Class II; D015375:Receptors, Interleukin-2; D007371:Interferon-gamma