Sequence analysis and expression of the mouse full-length vasoactive intestinal contractor/endothelin-2 gene (EDN2): comparison with the endothelin-1 gene (EDN1).
Vasoactive intestinal contractor (VIC)/endothelin-2 (ET2) is a vasoactive peptide hormone comprising 21 amino acids. The complete nucleotide sequence of the full-length gene encoding preproVIC (PPVIC) was determined. The PPVIC gene contains five exons that span 6 kb and shows a duplication on exons 2 and 3, coding for the VIC and VIC-like peptides respectively. Similarities between the genomic organization of the PPVIC/preproET2 and preproendothelin-1 genes suggest that the two are distantly related. PPVIC gene expression was observed in foetal and adult mouse intestine. The expression level in adults was approx. 10-fold higher than in the foetus, suggesting an involvement of VIC in intestinal development.
National Institute of Advanced Industrial Science and Technology, Ministry of Economy, Trade and Industry, Tsukuba, Ibaraki 305-8566, Japan. k.saida@aist.go.jp
Kaname
K
Saida
Naoko
N
Kometani
Tsuyoshi
T
Uchide
Youji
Y
Mitsui
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019332:Endothelin-1; D016232:Endothelins / Q000235:genetics*; D004847:Epithelial Cells / Q000378:metabolism; D005091:Exons; D015870:Gene Expression; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000196:embryology*; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000378:metabolism; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D019332:Endothelin-1; D016232:Endothelins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011498:Protein Precursors; C059568:vasoactive intestinal constrictor