A chip-based electrophoresis system with electrochemical detection and hydrodynamic injection.
A simple capillary electrophoresis system in the planar format that uses a new, hydrodynamic injection principle is described. The system was realized with poly(dimethylsiloxane)-glass chips and microdisk electrodes for amperometric detection. Using a double-tee injector, no precise voltage control of the electrolyte reservoirs was needed, thus making the microchip CE system more user-friendly. The analytical characteristics of chip-based CE-EC were evaluated using ascorbic acid as the model analyte. The reproducibility of migration time and signal height was expressed by relative standard deviations of 1.2% and 5.1%, respectively (n = 5). The limit of detection for ascorbic acid was approximately 5 microM at a signal-to-noise ratio of 3. Practical application concerning the determination of physiologically relevant compounds such as noradrenaline and L-dopa are discussed.
Backofen
Ulli
U
Department of Chemistry, University of Kansas, Lawrence 66045, USA.
Matysik
Frank-Michael
FM
Lunte
Craig E
CE
D001205:Ascorbic Acid / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000592:standards; D004867:Equipment Design; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001205:Ascorbic Acid