Three new human members of the lipid transfer/lipopolysaccharide binding protein family (LT/LBP).
We have identified three novel, rarely expressed human genes that encode new members of the lipid transfer/lipopolysaccharide binding protein (LT/LBP) gene family based on sequence homology. BPI and other members of the LT/LBP family are structurally related proteins capable of binding phospholipids and lipopolysaccharides. Real-time PCR studies indicate that BPIL1 and BPIL3 are highly expressed in hypertrophic tonsils. In situ hybridization analysis of BPIL2 shows prominent expression in skin specimens from psoriasis patients. BPIL1 and BPIL3 map to Chromosome 20q11; thus, these novel genes form a cluster with BPI and two other members of the LT/LBP gene family on the long arm of human Chr 20. BPIL2maps to Chr 22q13. The exon/intron organization of all three genes is highly conserved with that of BPI, suggesting evolution from a common ancestor.
Department of Functional Genomics, Hyseq Pharmaceuticals, 670 Almanor Ave., Sunnyvale, CA 94086, USA. jmulero@hyseq.com
Julio J
JJ
Mulero
Bryan J
BJ
Boyle
Shannon
S
Bradley
Jessica M
JM
Bright
Sarah T
ST
Nelken
Tam T
TT
Ho
Nancy K
NK
Mize
John D
JD
Childs
Dennis G
DG
Ballinger
John E
JE
Ford
Fabio
F
Rupp
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D003001:Cloning, Molecular; D003199:Computer Systems; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D011487:Protein Conformation
C466096:BPIFB2 protein, human; C466098:BPIFB6 protein, human; C466097:BPIFC protein, human; D002352:Carrier Proteins; D018076:DNA, Complementary