Preparative isolation and purification of calycosin from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography.
Calycosin was purified from an ethyl acetate extract of the root of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography. The separation was performed in two steps with a two-phase solvent system composed of n-hexane-chloroform-methanol-water (1:3:3:2, v/v). From 200 mg of the crude extract, 14.8 mg of calycosin was obtained at over 99% purity as determined by HPLC analysis, and its chemical structure was confirmed by MS, 1H and 13C nuclear magnetic resonance.
Ma
Xiaofeng
X
Peking University Modern Research Center for Traditional Chinese Medicine, Beijing 100083, China.
Zhang
Tianyou
T
Wei
Yun
Y
Tu
Pengfei
P
Chen
Yingjie
Y
Ito
Yoichiro
Y
D027883:Astragalus Plant / Q000737:chemistry*; D027885:Astragalus propinquus; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D007529:Isoflavones / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy
D007529:Isoflavones; C121707:7,3'-dihydroxy-4'-methoxyisoflavone