Direct analysis of nine pharmaceuticals in culture media by use of cartridge separation with electrospray mass spectrometric detection.
A 2-cm cartridge has been used for separation before electrospray mass spectrometric analysis of pharmaceutical compounds in cell culture media, alleviating the need for sample extraction and desalting procedures. Nine representative pharmaceuticals listed in the biopharmaceutical classification system (BCS) were chosen as the candidate compounds and Hank's balanced salt solution with Hepes buffer (HBSS-Hepes buffer) was used as the cell-culture medium in an effort to study permeability of chemicals through cell monolayers. Effects of several conditions, e.g. pH and buffer concentration in the mobile phase, flow rate, and temperature on separation efficiency were examined. The nine pharmaceuticals were separated within 2 min by use of a 2-cm C(8) cartridge. Relative standard deviations (RSD) from repeated analysis within the same day or over five days were 0.03-0.2% for retention times and 0.6-5.3% for peak areas; antipyrine was used as internal standard. Calibration curves based on peak-area measurements were linear over the range 0.1-20 micro mol L(-1). The HBSS-Hepes buffer did not interfere with separation and detection; identical separation and peak intensity were obtained when the samples were separately prepared in distilled water or in the culture medium.
Environmental Health Sciences Program, Department of Public Health Sciences, University of Alberta, Edmonton, Canada T6G 2G3. xingfang.li@ualberta.ca
Xing-Fang
XF
Li
Mingsheng
M
Ma
Yun K
YK
Tam
D000319:Adrenergic beta-Antagonists / Q000032:analysis; D002021:Buffers; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry*; D006571:Heterocyclic Compounds / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010539:Permeability; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013696:Temperature
D000319:Adrenergic beta-Antagonists; D002021:Buffers; D003470:Culture Media; D006571:Heterocyclic Compounds; D004364:Pharmaceutical Preparations