[The standardized techniques for preparing samples used in pathologic diagnostic transmission electron microscopy].
Transmission electron microscopy (TEM) has been used widespreadly in the field of diagnostic pathology. Based on the practical domestic condition and aimed at fully satisfying the needs of the pathologic diagnostic TEM, a set of standardized techniques were established for preparing different kinds of samples. The techniques included the preparation and storage of fixatives and embedding media, the requirement of sampling, the 5 h and 24 h embedding procedures for fresh specimens, the embedding procedure for suspension specimens and specimens collected from fine needle aspiration biopsy, as well as the procedures for preparing samples taken from paraffin embedded blocks or paraffin sections. In practical use, we found that the advantage of these techniques was that the ultrastructures of the tissues and cells were well preserved and they perfectly met the needs for diagnosis of tumors, kidney diseases, et al. The main points of the standardized techniques have been discussed in detail in this paper.
Yang
G
G
Electron Microscopy Laboratory, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Tang
X
X
Gan
D
D
D001943:Breast Neoplasms / Q000648:ultrastructure; D002294:Carcinoma, Squamous Cell / Q000648:ultrastructure; D003110:Colonic Neoplasms / Q000648:ultrastructure*; D006801:Humans; D008175:Lung Neoplasms / Q000648:ultrastructure*; D013048:Specimen Handling / Q000592:standards*