Serum protein synthesis in the early chick embryo.
Isolated yolk-sacs of chick embryos secreted serum proteins when incubated in buffered chick Ringer's solution. The presence of serum transferrin, two embryo-specific alpha-globulins, and a prealbumin were demonstrated by acrylamide gel analysis. Yolk-sacs from embryos explanted at 11-13 somites (40 hr preincubation) and cultured for 48 hr secreted in addition a protein with the mobility of serum albumin. Incubation of yolk-sacs in the presence of radioactive valine indicated that serum proteins were synthesized as early as the primitive streak stage. By incubating isolated yolk-sacs and embryos from 48-hr explants in the presence of radioactive valine, the synthesis of serum proteins was found to be restricted to the yolk-sac at this stage of development. Culturing explants on various nutrient proteins as well as protein starvation medium altered the relative synthesis of several serum proteins. We have proposed that morphological and biochemical changes in embryos resulting from altered nutrition may be mediated by the proteins of the serum.
College of Agriculture and Natural Resources, Department of Animal Genetics, University of Connecticut, Storrs, Connecticut 06268, USA.
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D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002642:Chick Embryo; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D005314:Embryonic and Fetal Development; D008967:Molecular Biology / Q000379:methods*; D009747:Nutritional Physiological Phenomena; D014633:Valine / Q000378:metabolism; D015017:Yolk Sac / Q000378:metabolism*
D001798:Blood Proteins; D003470:Culture Media; D014633:Valine