Flavonoids suppress the cytotoxicity of linoleic acid hydroperoxide toward PC12 cells.
The suppressive effect of flavonoids on the cytotoxicity of linoleic acid hydroperoxide (LOOH) toward rat phenochromocytoma PC12 cells was examined. The extent of cytotoxicity was shown on the basis of % survival determined by the trypan blue exclusion test. On preincubation of cells with either 3-hydroxyflavone, quercetin, or luteolin prior to LOOH exposure, the cytotoxicity was considerably suppressed. In contrast, on coincubation of cells with either eriodictyol, quercetin, kaempherol, luteolin, or 3-hydroxyflavone and LOOH, it was markedly suppressed. Regardless of incubation conditions, quercetin, 3-hydroxyflavone, and luteolin were thus more effective as protective agents against the cytotoxicity than the other flavonoids. These flavonoids further showed a suppressive effect on coincubation rather than on preincubation. These results suggest that such flavonoids are beneficial for cells under oxidative stress.
Sasaki
Naoko
N
Department of Biology, Faculty of Science and High Technology Research Center, Konan University Kobe, Japan.
Toda
Takeshi
T
Kaneko
Takao
T
Baba
Naomichi
N
Matsuo
Mitsuyoshi
M
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D008041:Linoleic Acids / Q000037:antagonists & inhibitors / Q000633:toxicity*; D008054:Lipid Peroxides / Q000037:antagonists & inhibitors / Q000633:toxicity*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000633:toxicity
D005419:Flavonoids; D008041:Linoleic Acids; D008054:Lipid Peroxides; D017382:Reactive Oxygen Species; C008285:linoleic acid hydroperoxide