Effects of oleanane-type triterpenoids from fabaceous plants on the expression of ICAM-1.
We examined the inhibitory effects of oleanane-type triterpenoids from fabaceous plants on the TNF-alpha-induced expression of cell adhesion molecules on THP-1 human monocytic leukemia cells and compared them with a glucocorticoid dexamethasone. In a cell-based ELISA, abrisapogenol E, soyasapogenols B and C, soyasapogenol B, kuzusapogenol B-methyl ester, and oleanolic acid significantly inhibited intercellular adhesion molecule (ICAM-1) expression. Moreover, these triterpenoids showed the same activity as dexamethasone. On the other hand, the absence of hydroxyl group at C-24 position of sapogenin rather to increase ICAM-1 expression compared with the untreated control. We concluded that the activity of oleanane saponins and sapogenins against ICAM-1 expression are dependent upon the position of the hydroxyl group, and in particular upon the status of the C-21 and C-24 positions and of the glycosyl group at C-3 position.
Immmunomodulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejeon.
Kyung-Seop
KS
Ahn
Jung-Hee
JH
Kim
Sei-Ryang
SR
Oh
Byung-Sun
BS
Min
Junei
J
Kinjo
Hyeong-Kyu
HK
Lee
D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D009828:Oleanolic Acid / Q000031:analogs & derivatives; D018514:Plant Structures / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D014315:Triterpenes; C413246:oleanane; D018799:Intercellular Adhesion Molecule-1; D009828:Oleanolic Acid