Differential expression of NAT1 translational repressor during development of bovine intramuscular adipocytes.
This study was undertaken to test for differential gene expression in intramuscular adipocytes during fat deposition of feedlot steers. Angus x Hereford steers (n = 50) were fed a high-energy concentrate ration ad libitum for 20 (n = 5), 86 (n = 15), 121 (n = 15), and 146 days (n = 15) to obtain various degrees of intramuscular adipocyte development. Carcass traits were significantly different (P < 0.05) between the groups. Intramuscular adipose tissue was excised from the longissimus dorsi and snap frozen in liquid nitrogen. Pooled samples of total RNA representing each group were analyzed by differential-display polymerase chain reaction using 200 primer combinations comprising 20 arbitrary (5') and 10 anchor (3') oligonucleotides. Bands (n = 70) representing putative differences among treatment groups were excised, sequenced, and subjected to BLAST homology search. From these, 40 contained significant homology to known genes. One was of particular interest, the translational repressor NAT1 (novel APOBEC-1 target-1). NAT1 mRNA was quantified in individual animals to confirm differential expression among treatment groups. Results indicate that NAT1 message is more abundant (P < 0.05) in intramuscular adipocytes of younger/leaner animals.
Childs
Kirby D
KD
Department of Animal Science, Oklahoma Agriculture Experiment Station, Oklahoma State University, Stillwater, Oklahoma 74078, USA.
Goad
David W
DW
Allan
Mark F
MF
Pomp
Daniel
D
Krehbiel
Clinton
C
Geisert
Rodney D
RD
Morgan
J Brad
JB
Malayer
Jerry R
JR
D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000166:cytology / Q000254:growth & development; D000821:Animal Feed; D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000254:growth & development; D002874:Chromosome Mapping; D004247:DNA; D002523:Edible Grain; D039603:Eukaryotic Initiation Factor-4G; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008460:Meat; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development*; D010448:Peptide Initiation Factors / Q000096:biosynthesis / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
C491300:EIF4G2 protein, human; D039603:Eukaryotic Initiation Factor-4G; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012097:Repressor Proteins; D004247:DNA