A physical map of the mouse genome.
A physical map of a genome is an essential guide for navigation, allowing the location of any gene or other landmark in the chromosomal DNA. We have constructed a physical map of the mouse genome that contains 296 contigs of overlapping bacterial clones and 16,992 unique markers. The mouse contigs were aligned to the human genome sequence on the basis of 51,486 homology matches, thus enabling use of the conserved synteny (correspondence between chromosome blocks) of the two genomes to accelerate construction of the mouse map. The map provides a framework for assembly of whole-genome shotgun sequence data, and a tile path of clones for generation of the reference sequence. Definition of the human-mouse alignment at this level of resolution enables identification of a mouse clone that corresponds to almost any position in the human genome. The human sequence may be used to facilitate construction of other mammalian genome maps using the same strategy.
Gregory
Simon G
SG
The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
Sekhon
Mandeep
M
Schein
Jacqueline
J
Zhao
Shaying
S
Osoegawa
Kazutoyo
K
Scott
Carol E
CE
Evans
Richard S
RS
Burridge
Paul W
PW
Cox
Tony V
TV
Fox
Christopher A
CA
Hutton
Richard D
RD
Mullenger
Ian R
IR
Phillips
Kimbly J
KJ
Smith
James
J
Stalker
Jim
J
Threadgold
Glen J
GJ
Birney
Ewan
E
Wylie
Kristine
K
Chinwalla
Asif
A
Wallis
John
J
Hillier
LaDeana
L
Carter
Jason
J
Gaige
Tony
T
Jaeger
Sara
S
Kremitzki
Colin
C
Layman
Dan
D
Maas
Jason
J
McGrane
Rebecca
R
Mead
Kelly
K
Walker
Rebecca
R
Jones
Steven
S
Smith
Michael
M
Asano
Jennifer
J
Bosdet
Ian
I
Chan
Susanna
S
Chittaranjan
Suganthi
S
Chiu
Readman
R
Fjell
Chris
C
Fuhrmann
Dan
D
Girn
Noreen
N
Gray
Catharine
C
Guin
Ran
R
Hsiao
Letticia
L
Krzywinski
Martin
M
Kutsche
Reta
R
Lee
Soo Sen
SS
Mathewson
Carrie
C
McLeavy
Candice
C
Messervier
Steve
S
Ness
Steven
S
Pandoh
Pawan
P
Prabhu
Anna-Liisa
AL
Saeedi
Parvaneh
P
Smailus
Duane
D
Spence
Lorraine
L
Stott
Jeff
J
Taylor
Sheryl
S
Terpstra
Wesley
W
Tsai
Miranda
M
Vardy
Jill
J
Wye
Natasja
N
Yang
George
G
Shatsman
Sofiya
S
Ayodeji
Bola
B
Geer
Keita
K
Tsegaye
Getahun
G
Shvartsbeyn
Alla
A
Gebregeorgis
Elizabeth
E
Krol
Margaret
M
Russell
Daniel
D
Overton
Larry
L
Malek
Joel A
JA
Holmes
Mike
M
Heaney
Michael
M
Shetty
Jyoti
J
Feldblyum
Tamara
T
Nierman
William C
WC
Catanese
Joseph J
JJ
Hubbard
Tim
T
Waterston
Robert H
RH
Rogers
Jane
J
de Jong
Pieter J
PJ
Fraser
Claire M
CM
Marra
Marco
M
McPherson
John D
JD
Bentley
David R
DR
D000818:Animals; D002875:Chromosomes / Q000235:genetics; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D020451:Contig Mapping / Q000379:methods; D016678:Genome; D015894:Genome, Human; D006801:Humans; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping / Q000379:methods*; D021101:Radiation Hybrid Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D026801:Synteny