Cutting edge: a novel role for Fas ligand in facilitating antigen acquisition by dendritic cells.
Fas ligand (FasL)-expressing tumor cells are found to effectively mediate rejection of the coinoculated FasL negative parental cells while having no effect on the growth of histologically distinct tumor cells. These observations indicate that FasL induces a specific immune response against Ag derived from FasL-bearing tumors and suggest a possible role for FasL in tumor Ag presentation. Indeed, tumor cells expressing FasL can efficiently interact with dendritic cells (DCs) and this interaction requires the expression of membrane-bound FasL on tumors and Fas on DCs. Moreover, DCs cocultured with FasL-expressing tumors are able to elicit a tumor-specific immune response in vivo, suggesting that DCs acquire tumor Ag during the Fas/FasL-mediated DC-tumor contact. These results identify a novel role for FasL in augmenting tumor-DC interactions and subsequent tumor Ag acquisition by DCs, and suggest that FasL-expressing tumor cells could be used to generate tumor-specific DC vaccines.
Division of Pathology, Chiba Cancer Center Research Institute, Graduate School of Medicine, Chiba University, Japan.
Yuji
Y
Tada
Jiyang
J
O-Wang
Yuichi
Y
Takiguchi
Koichiro
K
Tatsumi
Takayuki
T
Kuriyama
Seiji
S
Okada
Takeshi
T
Tokuhisa
Shigeru
S
Sakiyama
Masatoshi
M
Tagawa
D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism*; D018827:Carcinoma, Lewis Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D002450:Cell Communication / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism* / Q000637:transplantation; D053222:Fas Ligand Protein; D005260:Female; D006084:Graft Rejection / Q000276:immunology; D006131:Growth Inhibitors / Q000096:biosynthesis / Q000502:physiology; D008024:Ligands; D008175:Lung Neoplasms / Q000517:prevention & control / Q000556:secondary; D008546:Melanoma, Experimental / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011485:Protein Binding / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000378:metabolism / Q000637:transplantation; D019014:fas Receptor / Q000378:metabolism*
D000951:Antigens, Neoplasm; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D006131:Growth Inhibitors; D008024:Ligands; D008562:Membrane Glycoproteins; D019014:fas Receptor