Transient pseudo-isotachophoresis for sample concentration in capillary electrophoresis.
We show that many water miscible organic solvents such as acetonitrile, acetone and small alcohols can function as a terminating ion in transient isotachophoresis, which leads to sample concentration on the capillary. It is suggested that this method could be termed transient "pseudo-isotachophoresis" (pseudo-ITP). Because of their low conductivity, these water miscible organic solvents provide the high field strength necessary for band sharpening similar to that provided by the terminating ion. Salts, when present in such samples act briefly as leading ions, migrating rapidly in the organic solvent until they are slowed at the interface of the separation buffer. When the organic solvents are added to the sample, both the migrations as well as the stacking of the analytes are affected by the concentration of salts (leading ions) in the sample, similar to that observed in isotachophoresis. Our results show that this type of stacking offers good reproducibility and reliability for practical analysis. In practice, pseudo-ITP stacking is much easier to perform compared to that of true ITP with several added practical advantages as discussed.
Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC, USA. zshihabi@wfubmc.edu
Zakariya K
ZK
Shihabi
D000097:Acetonitriles / Q000737:chemistry / Q000494:pharmacology; D000409:Alanine / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000737:chemistry / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry / Q000494:pharmacology; D013806:Theophylline / Q000032:analysis
D000097:Acetonitriles; D012997:Solvents; D012965:Sodium Chloride; D013806:Theophylline; D000409:Alanine; C032159:acetonitrile