Sequence analysis and expression of the mouse full-length vasoactive intestinal contractor/endothelin-2 gene (EDN2): comparison with the endothelin-1 gene (EDN1).
Vasoactive intestinal contractor (VIC)/endothelin-2 (ET2) is a vasoactive peptide hormone comprising 21 amino acids. The complete nucleotide sequence of the full-length gene encoding preproVIC (PPVIC) was determined. The PPVIC gene contains five exons that span 6 kb and shows a duplication on exons 2 and 3, coding for the VIC and VIC-like peptides respectively. Similarities between the genomic organization of the PPVIC/preproET2 and preproendothelin-1 genes suggest that the two are distantly related. PPVIC gene expression was observed in foetal and adult mouse intestine. The expression level in adults was approx. 10-fold higher than in the foetus, suggesting an involvement of VIC in intestinal development.
Saida
Kaname
K
National Institute of Advanced Industrial Science and Technology, Ministry of Economy, Trade and Industry, Tsukuba, Ibaraki 305-8566, Japan. k.saida@aist.go.jp
Kometani
Naoko
N
Uchide
Tsuyoshi
T
Mitsui
Youji
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019332:Endothelin-1; D016232:Endothelins / Q000235:genetics*; D004847:Epithelial Cells / Q000378:metabolism; D005091:Exons; D015870:Gene Expression; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000196:embryology*; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000378:metabolism; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D019332:Endothelin-1; D016232:Endothelins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011498:Protein Precursors; C059568:vasoactive intestinal constrictor