Polyelectrolyte multilayer films modulate cytoskeletal organization in chondrosarcoma cells.
The aim of this study was to evaluate polyelectrolyte multilayer films as interfaces for implants. Polyelectrolyte multilayers were built up with different terminating layers by alternate deposition of oppositely charged polyelectrolytes on which chondrosarcoma (HCS-2/8) cells were grown in the presence of serum. Films formed by an increasing number of layers were investigated. The terminating layer was made of one of the following polyelectrolytes: poly-sodium-4-styrenesulfonate (PSS), poly-L-glutamic acid (PGA), poly-allylamine hydrochloride (PAH), or poly(L-lysine) (PLL). Cell viability, inflammatory response, adherence, and cytoskeletal organization were studied. Induction of interleukin-8 (IL-8) secretion was detected on PAH and PLL ending polyelectrolyte films. Early cellular adherence was enhanced with PGA, PAH, PLL, and, to a lower extent, PSS terminating layers. Adherence was independent of the number of layers constituting the films. The presence of actin filaments and vinculin focal adhesion spots was observed on PSS or PAH ending films. They were respectively partially and totally absent on PGA and PLL terminating multilayer architectures. For PLL ending films, vinculin and actin organization was clearly dependent on the number of deposited layers. The results of this study suggest that PSS ending multilayered films constitute a good interfacial micro-environment at the material surface for HCS-2/8 cells.
Vautier
Dominique
D
INSERM U 424, UFR Odontologie, Strasbourg, France. Dominique.Vautier@medicine.u-strasbg.fr
Karsten
Veronique
V
Egles
Christophe
C
Chluba
Johanna
J
Schaaf
Pierre
P
Voegel
Jean-Claude
JC
Ogier
Joëlle
J
D000199:Actins / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology; D001672:Biocompatible Materials / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D002813:Chondrosarcoma; D003599:Cytoskeleton / Q000378:metabolism*; D004573:Electrolytes / Q000378:metabolism*; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D011108:Polymers / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D016596:Vinculin / Q000378:metabolism
D000199:Actins; D001672:Biocompatible Materials; D004573:Electrolytes; D016209:Interleukin-8; D011108:Polymers; D016596:Vinculin