Pregenomic RNA is required for efficient incorporation of pol polyprotein into foamy virus capsids.
The foamy virus (FV) Pol polyprotein is translated independently of Gag from a spliced mRNA. This method of expression raises the question of how Pol is associated with the viral particle. Using a transient FV vector transfection system, it is shown that pregenomic RNA is required for efficient virion incorporation of functionally active Pol and that protein-protein interactions of Pol with Gag are not sufficient to complete particle assembly.
Institut für Virologie, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
Martin
M
Heinkelein
Cordula
C
Leurs
Matthias
M
Rammling
Katrin
K
Peters
Helmut
H
Hanenberg
Axel
A
Rethwilm
D002213:Capsid / Q000378:metabolism*; D015684:Gene Products, pol / Q000378:metabolism*; D012367:RNA, Viral / Q000502:physiology*; D016092:Spumavirus / Q000502:physiology*; D014771:Virion / Q000502:physiology; D019065:Virus Assembly
D015684:Gene Products, pol; D012367:RNA, Viral