[Observation on morphology and histology of Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. var. formosana (Boiss.) Shan et Yuan in the process of in vitro cultivation and polyploid induction].
OBJECTIVE
To explore the relationship between morphogenesis and histogenesis in the process of in vitro cultivation of Angelica dahurica f. var. formosana, and determine the best period for its polyploid induction.


METHODS
In the different periods of in vitro cultivation, the explants were obtained and their sections observed. When the adventitiousbuds began to differentiate, aqueous solution was applied for polyploid induction.


RESULTS
The adventitious buds were mainly externally originated and thus fit for polyploid induction. The most appropriate period for induction was around the 38th day of cultivation.


CONCLUSIONS
Induction treatment can be used as an effective biotechnological approach to adventitious buds in the process of tissue cultivation.
Hunan College of Traditional Chinese Medicine, Hunan Changsha 410004, China.
F
F
Peng
Y
Y
Guo
D029969:Angelica / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D046508:Culture Techniques; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D011123:Polyploidy; D013997:Time Factors