Characterization of an outbred pregnant mouse model of Neospora caninum infection.
Fetal loss and vertical transmission of Neospora caninum were evaluated in outbred Quackenbush (Qs) mice with respect to dose of parasites, N. caninum isolate, and route of injection. Mice were infected with NC-Liverpool or NC-SweB1 at day 5 or 8 of pregnancy with doses of 10(4), 10(6), or 10(7) parasites, through either a subcutaneous or intraperitoneal injection. Polymerase chain reaction was used to detect N. caninum in the brains of offspring, and enzyme-linked immunosorbent assay was used to analyze the maternal immune response. Vertical transmission occurred in mice given 10(6) NC-Liverpool at day 5 during gestation, and a significant (P < 0.05) maternal antibody response was observed in mice infected with NC-Liverpool or NC-SweB1 at days 5 and 8 of gestation. This study shows that outbred Qs mice are a useful model for the study of vertical transmission associated with N. caninum, as they display less clinical disease and pathogenesis than inbred mice and have large litters, which is advantageous when studying maternal transmission.
Quinn
Helen E
HE
Molecular Parasitology Unit, Department of Cell and Molecular Biology, University of Technology Sydney, Westbourne Street, Gore Hill, New South Wales 2065, Australia.
Miller
Catherine M D
CM
Ryce
Cheryl
C
Windsor
Peter A
PA
Ellis
John T
JT
D000818:Animals; D000831:Animals, Newborn; D021461:Animals, Outbred Strains; D000913:Antibodies, Protozoan / Q000032:analysis; D001921:Brain / Q000469:parasitology; D003048:Coccidiosis / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D016054:DNA, Protozoan / Q000032:analysis; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D051379:Mice; D008807:Mice, Inbred BALB C; D018688:Neospora / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000469:parasitology / Q000662:veterinary*; D012376:Rodent Diseases / Q000469:parasitology / Q000635:transmission*
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan