[Group A bovine rotavirus: diagnosis and antigenic characterization of strains circulating in the Argentine Republic, 1994-1999].
Group A Bovine Rotavirus (BRV) has been identified as a major cause of neonatal diarrhea in cattle. The study was aimed to determine the prevalence of BRV and to antigenically characterize the G-types of circulating strains in dairy and beef herds in Argentina. A total of 1129 stool samples from diarrheic calves was analyzed from 1994 to 1999. The samples were initially screened for RV by ELISA and PAGE, and then G-typed using monoclonal antibodies (Mab) directed against G1, G2, G3, G6 and G10-specific epitopes. Forty percent (452/1129) of the samples were positive for RV by ELISA, while 24.7% (279/1129) were also positive for PAGE. VP7 was detected in the 70.5% (319/452) of the positive samples using a broadly reactive Mab (C60); 32.6% (104/319) were G6, 15.4% (49/319) were G10, and 6% (19/319) were G1. However, 46.1% (147/319) of the samples remained untypable. Rotavirus diarrhea prevalences were comparable in beef and dairy herds (87.3% and a 74.4%, respectively). Finally, G6 was the most prevalent G-type circulating in beef herds while G10 prevailed in dairy herds. A better understanding of RV epidemiology will contribute to the optimization of current vaccines and prevention programs of RV diarrhea in calves.
Instituto de Virología, CICVyA, INTA Castelar, CC 77, 1708 Morón, Pcia de Buenos Aires, Argentina.
V
V
Costantini
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V
Parreño
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M
Barrandeguy
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G
Combessies
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JC
Bardón
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A
Odeón
M
M
Leunda
L
L
Saif
F
F
Fernández
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D001118:Argentina / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D015995:Prevalence; D012401:Rotavirus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014764:Viral Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014764:Viral Proteins