Peptidomics-based approach reveals the secretion of the 29-residue COOH-terminal fragment of the putative tumor suppressor protein DMBT1 from pancreatic adenocarcinoma cell lines.
Deleted in malignant brain tumors 1 is a putative tumor suppressor protein in brain, lung, esophageal,gastric, and colorectal cancer. Here we report the mass spectrometric identification of a 3335 Da peptide, which was found in serum-free conditioned medium from 5 of 15 pancreatic adenocarcinoma cell lines but not from 35 carcinoma cell lines and 2 nonmalignant pancreatic duct cell lines. The peptide was the 29 COOH-terminal amino acids from deleted in malignant brain tumors 1. It is suggested that the peptide is generated inside the cells by limited proteolysis and extracellularly secreted. Our peptidomics-based approach will help screen candidate marker peptides for a particular type of cancer.
Growth Factor Division, National Cancer Center Research Institute, Tokyo 104-0045, Japan. ksasaki@ncc.go.jp
Kazuki
K
Sasaki
Kae
K
Sato
Yasuto
Y
Akiyama
Kazuyoshi
K
Yanagihara
Masaaki
M
Oka
Ken
K
Yamaguchi
D000230:Adenocarcinoma / Q000737:chemistry / Q000378:metabolism*; D000373:Agglutinins; D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins; D016895:Culture Media, Serum-Free; D004268:DNA-Binding Proteins; D006801:Humans; D008969:Molecular Sequence Data; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D019151:Peptide Library; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
D000373:Agglutinins; D002135:Calcium-Binding Proteins; D016895:Culture Media, Serum-Free; C108174:DMBT1 protein, human; D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D019151:Peptide Library; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins