Epitope distribution of randomly established monoclonal antibodies against human type II DNA topoisomerases.
The epitopes of about 100 monoclonal antibodies against human type II DNA topoisomerase were mapped along the enzyme molecules. Although they were randomly and independently established, epitope sites were unevenly distributed the toward N-terminal or C-terminal region. We suggest that the central catalytic domain is hidden inside the molecule and inaccessible to the antigen recognition sites. Using antibodies, we demonstrate the distinct localization of isoforms of Topo II in cultured cells. Some particularly useful antibodies are listed.
Nagoya University Graduate School & Faculty of Medicine, 466-8550, Japan.
Ayako
A
Sakaguchi
Mitsuko
M
Miyaike
Kazufumi
K
Kuroda
Naohito
N
Nozaki
Masato
M
Tanaka
Makoto
M
Hibino
Yasuo
Y
Fujii
Satoshi
S
Kato
Akihiko
A
Kikuchi
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D001483:Base Sequence; D017931:DNA Primers; D004250:DNA Topoisomerases, Type II / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D000939:Epitopes; D004250:DNA Topoisomerases, Type II