Beta-1,2-mannosylation of Candida albicans mannoproteins and glycolipids differs with growth temperature and serotype.
Increasing the growth temperature from 28 to 37 degrees C reduced the expression of beta-1,2-oligomannoside epitopes on mannoproteins of Candida albicans serotypes A and B. In contrast, beta-1,2-mannosylation of phospholipomannan (PLM) remained constant despite a slight decrease in the relative molecular weight (M(r)) of this compound. At all growth temperatures investigated, serotype A PLM displayed an M(r) and an antigenicity different from those of serotype B PLM when they were tested with a panel of monoclonal antibodies.
Laboratoire de Mycologie Fondamentale et Appliquée, INSERM EPI 9915, Faculté de Médecine, Pôle Recherche, 59045 Lille Cedex, France.
P A
PA
Trinel
T
T
Jouault
J E
JE
Cutler
D
D
Poulain
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal / Q000737:chemistry / Q000378:metabolism; D002176:Candida albicans / Q000145:classification / Q000254:growth & development / Q000276:immunology / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006017:Glycolipids / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008970:Molecular Weight; D012703:Serotyping; D013696:Temperature
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D005656:Fungal Proteins; D006017:Glycolipids; D008562:Membrane Glycoproteins; C025363:mannoproteins; C400188:phospholipomannan; D008358:Mannose