Convenient and rapid detection of pesticides in extracts of sheep wool.
A relatively simple, rapid extraction technique based on acetonitrile was combined with the use of screen-printed electrodes bearing cholinesterases to detect organo-phosphate pesticides from an otherwise intractable matrix, sheep wool. It proved possible to separate, for convenience, the exposure of the electrodes from measurement of their (inhibited) activity. The electrodes were used once and then discarded. Estimation of the extent of inhibition is dependent on reference to the activity of control electrodes. The presence of pesticides in the extracts could be detected with any of three commonly available cholinesterases but the most sensitive enzyme was butyryl cholinesterase from horse serum.
Food Systems and Technology, AgResearch Ltd., Private Bag 11008, Palmerston North, New Zealand.
W A
WA
Collier
M
M
Clear
A L
AL
Hart
D000097:Acetonitriles / Q000737:chemistry*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002709:Chlorfenvinphos / Q000032:analysis; D002800:Cholinesterase Inhibitors / Q000032:analysis; D002802:Cholinesterases / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D003976:Diazinon / Q000032:analysis; D004566:Electrodes; D004867:Equipment Design; D007306:Insecticides / Q000032:analysis*; D010261:Paraoxon / Q000032:analysis; D010575:Pesticides / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep; D014935:Wool / Q000737:chemistry*
D000097:Acetonitriles; D002800:Cholinesterase Inhibitors; D020099:Coated Materials, Biocompatible; D007306:Insecticides; D010575:Pesticides; D002802:Cholinesterases; D002709:Chlorfenvinphos; D010261:Paraoxon; D003976:Diazinon; C032159:acetonitrile