title: Mutations in the aph(2")-Ic gene are responsible for increased levels of aminoglycoside resistance.
abstract: Random PCR mutagenesis of the enterococcal aph(2")-Ic gene followed by selection for mutant enzymes that confer enhanced levels of aminoglycoside resistance resulted in mutants of APH(2")-Ic with His-258-Leu and Phe-108-Leu substitutions, all of which conferred rises in the MICs of several aminoglycosides. The mutated residues are located outside conserved regions of aminoglycoside phosphotransferases.
authors.affiliation: Division of Infectious Diseases, Wayne State University, Detroit, Michigan 48201, USA.
authors.forename: Hae Kyung
authors.initials: HK
authors.lastname: Lee
authors.forename: Sergei B
authors.initials: SB
authors.lastname: Vakulenko
authors.forename: Don B
authors.initials: DB
authors.lastname: Clewell
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Lerner
authors.forename: Joseph W
authors.initials: JW
authors.lastname: Chow
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins; D024881:Drug Resistance, Bacterial; D016983:Enterococcus / Q000187:drug effects* / Q000201:enzymology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C425933:APH(2'')-Ic protein, Enterococcus faecalis; D017853:Phosphotransferases (Alcohol Group Acceptor)