title: Pear transformed with a lytic peptide gene for disease control affects nontarget organism, pear psylla (Homoptera: Psyllidae).
abstract: The biology and behavior of pear psylla, Cacopsylla pyricola Foerster, on a transgenic clone of 'Bartlett' pear, Pyrus communis L., containing a synthetic antimicrobial gene, D5C1, was compared with that of a nontransgenic parental clone to determine whether there were any nontarget effects. The gene construct also contained the marker gene nptII (aminoglycoside 3'-phosphotransferase II) that encodes for antibiotic resistance to identify transformed plants. The purpose of the original transformation was to enhance pear resistance to the bacterial disease fireblight caused by Erwinia amylovora (Burr.) Winslow et al. The biology and behavior of pear psylla on a transgenic clone were compared with a nontransgenic parental pear clone in short- (< or = 7-d) and long-term (32-d) studies. Short-term studies indicated pear psylla adults preferred to settle and oviposit, and nymphs fed more and developed slightly faster, on transgenic pear compared with nontransgenic pear. In contrast, a long-term study on psylla colony development showed considerably fewer eggs, nymphs, and adults were produced on transgenic pear. Although adults reared on transgenic pear did not have weight affected, females produced fewer eggs and nymphal hatch was significantly reduced on the transgenic pear clone. Our results suggest that pear psylla biology and behavior are initially enhanced on this transgenic pear clone. However, chronic exposure of psylla populations to transformed pear plants that express the nptII marker and lytic peptide genes had detrimental effects on pear psylla reproductive biology. Field studies would be required to determine the specific effects of each gene on pear psylla biology and behavior and whether these effects would be expressed under natural conditions. The four-fold reduction in psylla population levels that resulted on this disease resistant transgenic pear line would be an added benefit to a pear integrated pest management (IPM) program. Overall, this study demonstrates that genetically altering plants to control one particular organism can have unintentional yet beneficial effects against other nontarget pest organisms in agricultural crops.
authors.affiliation: USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV 25430, USA. gputerka@afrs.ars.usda.gov
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Puterka
authors.forename: Chris
authors.initials: C
authors.lastname: Bocchetti
authors.forename: Phat
authors.initials: P
authors.lastname: Dang
authors.forename: R L
authors.initials: RL
authors.lastname: Bell
authors.forename: Ralph
authors.initials: R
authors.lastname: Scorza
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001681:Biological Assay; D004885:Erwinia / Q000235:genetics*; D005247:Feeding Behavior; D005260:Female; D029624:Ferns / Q000254:growth & development*; D005798:Genes, Bacterial / Q000502:physiology*; D006430:Hemiptera / Q000502:physiology*; D009758:Nymph / Q000254:growth & development; D010058:Oviposition; D010935:Plant Diseases; D030821:Plants, Genetically Modified; D013997:Time Factors; D014170:Transformation, Genetic
chemical_list: D001426:Bacterial Proteins