title: Fluorine electron double resonance imaging for 19F MRI in low magnetic fields.
abstract: This work demonstrates the feasibility of generating fluorine NMR images at a very low magnetic field of 0.015 T by making use of the Overhauser enhancement of (19)F NMR signal brought about by a stable, water-soluble, narrow-line paramagnetic contrast agent. The enhancement in the (19)F NMR images depends on the concentration of the single electron contrast agent, the pO(2), and the electron paramagnetic resonance (EPR) irradiation power. The applicability of this technique for (19)F NMR imaging is demonstrated with phantom samples, where a time resolution of 4-10 min is achieved. Proton electron double resonance imaging (PEDRI) and fluorine electron double resonance imaging (FEDRI) images were also obtained from rat kidneys ex vivo, perfused with 10 mM Oxo63 and 10 M trifluoroacetic acid. The spatial and temporal resolutions of these images are comparable to those obtained at magnetic fields 2-3 orders of magnitude larger. Constant NMR frequency (628 kHz) operation permits both FEDRI and PEDRI of identical slices without removing the object under investigation. This feasibility of coregistration of proton-based anatomical PEDRI image with physiological FEDRI image offers good potential for studying fluorine-containing tracers.
authors.affiliation: Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Ramachandran
authors.initials: R
authors.lastname: Murugesan
authors.forename: Sean
authors.initials: S
authors.lastname: English
authors.forename: Koen
authors.initials: K
authors.lastname: Reijnders
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Yamada
authors.forename: John A
authors.initials: JA
authors.lastname: Cook
authors.forename: James B
authors.initials: JB
authors.lastname: Mitchell
authors.forename: Sankaran
authors.initials: S
authors.lastname: Subramanian
authors.forename: Murali C
authors.initials: MC
authors.lastname: Krishna
mesh_terms: D000818:Animals; D003287:Contrast Media; D005260:Female; D005461:Fluorine; D007668:Kidney / Q000033:anatomy & histology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D019047:Phantoms, Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003287:Contrast Media; D005461:Fluorine