Cloning and characterization of an aromatic amino acid and leucine permease of Penicillium chrysogenum.
The gene encoding the amino acid permease ArlP (Aromatic and leucine Permease) was isolated from the filamentous fungus Penicillium chrysogenum after PCR using degenerated oligonucleotides based on conserved regions of fungal amino acid permeases. The cDNA clone was used for expression of the permease in Saccharomyces cerevisiae M4054, which is defective in the general amino acid permease Gap1. Upon overexpression, an increase in the uptake of L-tyrosine, L-phenylalanine, L-tryptophan and L-leucine was observed. Further competition experiments indicate that ArlP recognizes neutral and aromatic amino acids with an unbranched beta-carbon atom.
Trip
Hein
H
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
Evers
Melchior E
ME
Konings
Wil N
WN
Driessen
Arnold J  M
AJ
D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D007930:Leucine / Q000378:metabolism; D008969:Molecular Sequence Data; D010408:Penicillium chrysogenum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D010649:Phenylalanine / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment; D014364:Tryptophan / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D026905:Amino Acid Transport Systems; D018076:DNA, Complementary; D014443:Tyrosine; D010649:Phenylalanine; D014364:Tryptophan; D007930:Leucine