title: Comparative study between elisa IgG, IgM and PCR in diagnosing and studying toxoplasmosis in Qualyobia Governorate, Egypt.
abstract: ELISA IgG, IgM antibodies and PCR for toxoplasmosis were performed on 55 women with complicated gestation and their babies. Besides, ELISA IgG and IgM were applied on 27 uncomplicated gestation (mothers & babies) and 152 randomly selected individuals. Seropositivity to specific IgG antibodies was 36.4%, 59.2% and 57.9% and for IgM was 27.3%, 7.4% and 10.5% in complicated gestation. uncomplicated gestation and random population respectively. PCR was positive in 20%, 50% and 60% of mothers with abortion, premature deliveries and deliveries of babies with congenital anomalies respectively. 55.5% and 40% were found seropositive for IgG from normal full term babies and abnormal babies. 13% of abnormal babies were IgM positive and 46.6% were PCR positive from the same group.
authors.affiliation: Department of Medical Parasitology, Faculty of Medicine, Benha Branch of Zagazig University, Benha, Egypt.
authors.forename: Amany F
authors.initials: AF
authors.lastname: el Fakahany
authors.forename: Amina I
authors.initials: AI
authors.lastname: Abdel-Maboud
authors.forename: Manal F
authors.initials: MF
authors.lastname: el-Garhy
authors.forename: Maysa A
authors.initials: MA
authors.lastname: Eraky
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D016022:Case-Control Studies; D016054:DNA, Protozoan / Q000097:blood; D004534:Egypt / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005312:Fetal Blood / Q000276:immunology / Q000469:parasitology; D005315:Fetal Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D007231:Infant, Newborn; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis / Q000453:epidemiology; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000453:epidemiology; D014125:Toxoplasmosis, Congenital
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M