Effects of butyltins on human 5alpha-reductase type 1 and type 2 activity.
Butyltins are widely used biocides and accumulate in the food chain. Tributyltin is an imposex-inducing endocrine disrupter in animals. Imposex is characterized by the development of additional male sex organs on females. In a previous study, we identified tributyltin as an inhibitor of human cytochrom P450 aromatase activity. The present work focuses on the impact of butyltins on human androgen metabolism. Activation of androgens is mediated by two human 5alpha-reductase isoenzymes. 5alpha-Reductase type 1 was completely inhibited by tributyltin chloride (IC50=19.9 microM) and dibutyltin dichloride (IC50=32.9 microM), whereas 5alpha-reductase type 2 was only inhibited by tributyltin chloride (IC50=10.8 microM). Both isoenzymes were not affected by tetrabutyltin or monobutyltin indicating that at least two butyl groups bound to the positively charged Sn are required for the interaction of butyltins with the enzymes. Tributyltin inhibited 5alpha-reductase type 1 competitively whereas an irreversible inhibition was evident for the type 2 isoenzyme. In contrast to the distinct effects on 5alpha-reductases, reductive brain 17beta-hydroxysteroid dehydrogenase activity was not inhibited by any butyltin. Insufficient activation of androgens is responsible for developmental disorders of the male reproductive system such as hypospadias. At pharmacologic levels butyltins might contribute to the onset of developmental disorders of the male reproductive system. At present, however, it is unknown whether these levels are reached after acute or chronic exposure to butyltins.
Doering
Dagmar D
DD
Department of Clinical Biochemistry, University of Bonn, Sigmund-Freud-Strasse 25, Bonn, Germany.
Steckelbroeck
Stephan
S
Doering
Thomas
T
Klingmüller
Dietrich
D
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism; D058891:5-alpha Reductase Inhibitors; D000735:Androstenedione / Q000378:metabolism; D001921:Brain / Q000201:enzymology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism; D009947:Organotin Compounds; D020033:Protein Isoforms / Q000037:antagonists & inhibitors / Q000378:metabolism; D014220:Trialkyltin Compounds / Q000494:pharmacology*
D058891:5-alpha Reductase Inhibitors; D009947:Organotin Compounds; D020033:Protein Isoforms; D014220:Trialkyltin Compounds; D000735:Androstenedione; C011559:tributyltin; C048581:monotributyltin trichloride; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; C020735:dibutyldichlorotin