Improvement of the functions of osteoblasts seeded on modified poly(D,L-lactic acid) with poly(aspartic acid).
One of the challenges in the field of tissue engineering is the development of biomaterial/cell interactions. For the purposes of the present study, two molecular weights of poly(aspartic acid) (PASP) were used to modify poly(D,L-lactic acid) (PDLLA) films in order to enhance their cell affinity. The properties of the PDLLA-modified surfaces and the controls were investigated by water contact angle measurement and electron spectroscopy for chemical analysis (ESCA). These data reflect the change in the biocompatibility of modified PDLLA surfaces. Then rat osteoblasts were seeded onto these modified surfaces and on controls to examine their effects on cell adhesion and proliferation. Cell morphologies on these surfaces were studied by scanning electron microscopy (SEM), and cell viability was evaluated with a MTT assay. In addition, differentiated cell function was assessed by measuring alkaline phosphatase (ALP) activity. The results suggest that PASP-modified surfaces may enhance the interactions between osteoblasts and PDLLA films.
Cai
Kaiyong
K
Research Institute of Polymeric Materials, Tianjin University, Tianjin, 300072, Peoples Republic of China.
Yao
Kangde
K
Hou
Xin
X
Wang
Yaquan
Y
Hou
Yongjiang
Y
Yang
Zhiming
Z
Li
Xiuqiong
X
Xie
Huiqi
H
D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018914:Cells, Immobilized; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing; D008567:Membranes, Artificial; D010006:Osteoblasts / Q000187:drug effects* / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D013778:Tetrazolium Salts; D013844:Thiazoles
D008567:Membranes, Artificial; D010455:Peptides; D011091:Polyesters; D011108:Polymers; D011506:Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; C017645:polyaspartate; D019344:Lactic Acid; C033616:poly(lactide); D000469:Alkaline Phosphatase; C022616:thiazolyl blue