title: -11 Mutation in the ampC promoter increasing resistance to beta-lactams in a clinical Escherichia coli strain.
abstract: A mutation was discovered in the Pribnow box of the ampC promoter in a clinical Escherichia coli strain. This -11 C-to-T transition created a perfect homology with the -10 consensus sequence. The new promoter was cloned upstream of the cat gene of pKK232-8 and induced a sixfold increase in promoter strength.
authors.affiliation: Laboratoire de Bactériologie-Virologie, Hygiène hospitalière, CHU, Université de Nantes, France. stephane.corvec@chu-nantes.fr
authors.forename: S
authors.initials: S
authors.lastname: Corvec
authors.forename: N
authors.initials: N
authors.lastname: Caroff
authors.forename: E
authors.initials: E
authors.lastname: Espaze
authors.forename: J
authors.initials: J
authors.lastname: Marraillac
authors.forename: A
authors.initials: A
authors.lastname: Reynaud
mesh_terms: D001426:Bacterial Proteins; D001483:Base Sequence; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D011401:Promoter Regions, Genetic; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D047090:beta-Lactams; C048795:AmpC beta-lactamases; D001618:beta-Lactamases