title: Five age-dependently expressed genes in mouse brain revealed by the fluorescence differential display-PCR technique.
abstract: We used a fluorescence differential display-PCR (FDD-PCR) technique to analyze the genes expressed in mouse brains collected at nine different developmental stages ranging from 3 days to 15 months after birth, and 5 age-dependently expressed genes were found. Age-dependent expression of each of these 5 genes was confirmed by quantitative real-time PCR analysis. Of the 5 genes, 4 (B1-B4) had high homology with the nucleotide sequences of cDNA clones of known mouse genes (myelin proteolipid protein, transferrin, embryo cDNA from the RIKEN full-length enriched library, and protein tyrosine phosphatase), and the rest (B5) with expressed sequence tags of an unknown gene. Sequencing analysis of the full-length cDNA constructed based on the B5 sequence demonstrated that the gene product of B5 was identical to G-substrate, a specific substrate for cGMP-dependent protein kinase. The expression patterns of known genes obtained in our study may provide a further opportunity to investigate the biological and physiological roles of the proteins they encode.
authors.affiliation: Department of Forensic Medicine, Fukui Medical University, Matsuoka-cho, Fukui 910-1193, Japan. ireiko@fmsrsa.fukui-med.ac.jp
authors.forename: R
authors.initials: R
authors.lastname: Iida
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: E
authors.initials: E
authors.lastname: Tsubota
authors.forename: H
authors.initials: H
authors.lastname: Takatsuka
authors.forename: M
authors.initials: M
authors.lastname: Masuyama
authors.forename: T
authors.initials: T
authors.lastname: Matsuki
authors.forename: K
authors.initials: K
authors.lastname: Kishi
mesh_terms: D000367:Age Factors; D000818:Animals; D001921:Brain / Q000378:metabolism*; D018076:DNA, Complementary / Q000737:chemistry; D005453:Fluorescence; D020869:Gene Expression Profiling; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D018076:DNA, Complementary