title: Bean [alpha]-Amylase Inhibitor Confers Resistance to the Pea Weevil (Bruchus pisorum) in Transgenic Peas (Pisum sativum L.).
abstract: Bruchid larvae cause major losses of grain legume crops through-out the world. Some bruchid species, such as the cowpea weevil and the azuki bean weevil, are pests that damage stored seeds. Others, such as the pea weevil (Bruchus pisorum), attack the crop growing in the field. We transferred the cDNA encoding the [alpha]-amylase inhibitor ([alpha]-AI) found in the seeds of the common bean (Phaseolus vulgaris) into pea (Pisum sativum) using Agrobacterium-mediated transformation. Expression was driven by the promoter of phytohemagglutinin, another bean seed protein. The [alpha]-amylase inhibitor gene was stably expressed in the transgenic pea seeds at least to the T5 seed generation, and [alpha]-AI accumulated in the seeds up to 3% of soluble protein. This level is somewhat higher than that normally found in beans, which contain 1 to 2% [alpha]-AI. In the T5 seed generation the development of pea weevil larvae was blocked at an early stage. Seed damage was minimal and seed yield was not significantly reduced in the transgenic plants. These results confirm the feasibility of protecting other grain legumes such as lentils, mungbean, groundnuts, and chickpeas against a variety of bruchids using the same approach. Although [alpha]-AI also inhibits human [alpha]-amylase, cooked peas should not have a negative impact on human energy metabolism.
authors.affiliation: Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, GPO Box 1600, Canberra ACT 2601, Australia.
authors.forename: H. E.
authors.initials: HE
authors.lastname: Schroeder
authors.forename: S.
authors.initials: S
authors.lastname: Gollasch
authors.forename: A.
authors.initials: A
authors.lastname: Moore
authors.forename: L. M.
authors.initials: LM
authors.lastname: Tabe
authors.forename: S.
authors.initials: S
authors.lastname: Craig
authors.forename: D. C.
authors.initials: DC
authors.lastname: Hardie
authors.forename: M. J.
authors.initials: MJ
authors.lastname: Chrispeels
authors.forename: D.
authors.initials: D
authors.lastname: Spencer
authors.forename: TJV.
authors.initials: T
authors.lastname: Higgins