Sesquiterpenes with hepatoprotective activity from Cnidium monnieri on tacrine-induced cytotoxicity in Hep G2 cells.
Bioassay-guided fractionation of the EtOH extract of Cnidium monnieri (Apiaceae) furnished two hepatoprotective sesquiterpenes, torilin (1) and torilolone (2), together with a new derivative, 1-hydroxytorilin (3). Compounds 1 and 2 showed hepatoprotective effects on tacrine-induced cytotoxicity in human liver-derived Hep G2 cells. The EC50 values of compounds 1 and 2 were 20.6 +/- 1.86 (P < 0.01) and 3.6 +/- 0.1 (P < 0.01) microM, respectively. Silybin as a positive control showed an EC50 value of 69.0 +/- 3.4 microM.
Oh
Hyuncheol
H
Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Korea.
Kim
Jung-Sik
JS
Song
Eun-Kyoung
EK
Cho
Hyun
H
Kim
Do-Hoon
DH
Park
Sung-Eun
SE
Lee
Ho-Sub
HS
Kim
Youn-Chul
YC
D031048:Cnidium / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D012717:Sesquiterpenes / Q000302:isolation & purification* / Q000494:pharmacology*; D013619:Tacrine / Q000037:antagonists & inhibitors* / Q000633:toxicity; D014407:Tumor Cells, Cultured
D010936:Plant Extracts; D012717:Sesquiterpenes; D013619:Tacrine