title: Myostatin and TGF-beta2 gene expression patterns in response to in ovo administration of rhIGF-I during chicken embryonic development.
abstract: The objective of the study was to evaluate the impact of in ovo administration of recombinant human insulin-like growth factor-I (rhIGF-I) on myostatin and transforming growth factor-beta2 (TGF-beta2) gene expression during chicken embryogenesis with emphasis on skeletal muscle development. Eggs were injected once with 100 ng rh IGF-I in 10 mM acetic acid, 0.1% BSA per embryo on day 3 of embryonic development. Total RNA was isolated from whole embryos on each of embryonic days (E) 0 to 6 (n = 6 per day/per treatment), from thoracic/abdominal halves of the embryo at E 7 to 8 (n = 6 per day/per treatment), and from pectoralis muscle tissues at E 9 to 20 (n = 4 per day/per treatment). Reverse-transcription polymerase chain reaction (RT-PCR) was used to synthesize cDNAs. Myostatin mRNA isolated from pectoralis muscles of the rhIGF-I treated group increased on E 10 (approximately 2.5 fold) and remained high through E 13, whereas myostatin mRNA from control pectoralis muscles increased at E 9 and remained high until E 12. TGF-beta2 gene expression from in ovo rhIGF-I treated pectoralis muscles dramatically increased at E 13 (approximately 2.5 fold), in contrast to E 14 from control pectoralis muscle, and gradually declined through E 16. Our results demonstrate that in ovo administration of rhIGF-I on E 3 may alter developmental expression patterns of myostatin and TGF-beta2 genes.
authors.affiliation: Division of Animal and Veterinary Sciences, West Virginia University, Morgantown 26506, USA.
authors.forename: Hakan
authors.initials: H
authors.lastname: Kocamis
authors.forename: Scott A
authors.initials: SA
authors.lastname: Gahr
authors.forename: Jennifer
authors.initials: J
authors.lastname: Richter
authors.forename: Diana C
authors.initials: DC
authors.lastname: Kirkpatrick-Keller
authors.forename: John
authors.initials: J
authors.lastname: Killefer
mesh_terms: D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D055435:Myostatin; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000235:genetics*; D053781:Transforming Growth Factor beta2
chemical_list: C525911:MSTN protein, human; D055435:Myostatin; D012333:RNA, Messenger; D011994:Recombinant Proteins; C509041:TGFB2 protein, human; D016212:Transforming Growth Factor beta; D053781:Transforming Growth Factor beta2; D007334:Insulin-Like Growth Factor I