Lysosomal glycosidases and their natural substrates in major salivary glands of hamsters treated with 7,12-dimethylbenzanthracene (DMBA).
Oro-maxillofacial diseases may influence structure and function of salivary glands. In this study, 32 hamsters were treated with topical application of 7,12-dimethylbenzanthracene (DMBA) on the buccal pouch. After 16 weeks, the animals were killed and the major salivary glands extracted. The activities of some lysosomal glycosidases and their natural substrates were measured to understand how the carcinogenetic stress and the inflammatory reaction could influence the physiology of the salivary glands. Large differences were observed in lysosomal activities among treated and untreated animals. Similarly, large differences were shown in the concentration of natural substrates, including sialic acids. These results suggest that inflammation and/or tumors induce profound changes in the biology of the salivary glands.
Giuliani
Michele
M
Dental School, Catholic University of the Sacred Heart, School of Dentistry, Largo A Gemelli 8, Rome, Italy. iclod@rm.unicatt.it
Antuzzi
Daniela
D
Lajolo
Carlo
C
Vitaioli
Lucia
L
Tommasoni
Daniele
D
Ricci
Roberta
R
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage / Q000494:pharmacology*; D000287:Administration, Topical; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000494:pharmacology*; D006224:Cricetinae; D005260:Female; D006026:Glycoside Hydrolases / Q000187:drug effects / Q000378:metabolism*; D006595:Hexosamines / Q000032:analysis; D007249:Inflammation; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D011506:Proteins; D012469:Salivary Glands / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D012737:Sex Factors; D012794:Sialic Acids / Q000032:analysis
D002273:Carcinogens; D006595:Hexosamines; D011506:Proteins; D012794:Sialic Acids; C018589:lysosomal proteins; D015127:9,10-Dimethyl-1,2-benzanthracene; D006026:Glycoside Hydrolases