title: The influence of chemical sympathectomy on pain responsivity and alpha 2-adrenergic antinociception in neuropathic animals.
abstract: We studied the effect of chemical sympathectomy by 6-hydroxydopamine (6-OHDA) on pain behavior and alpha(2)-adrenergic antinociception in rats with a spinal nerve ligation-induced neuropathy. For assessment of alpha(2)-adrenergic antinociception, the rats were treated systemically with two alpha(2)-adrenoceptor agonists, one of which only poorly (MPV-2426) and the other very well (dexmedetomidine) penetrates the blood-brain barrier. Moreover, the effect of MPV-2426 on spontaneous activity of dorsal root nerve fibers proximal to the nerve injury was determined. Systemic treatment with 6-OHDA produced a marked decrease in immunocytochemical labeling of sympathetic nerve fibers in the skin but it produced no marked change in basal pain sensitivity to mechanical stimulation either in neuropathic or sham-operated animals. Systemic administration of MPV-2426 and dexmedetomidine produced a dose-dependent tactile antiallodynic effect in neuropathic animals. Intraplantar injection of MPV-2426 had an identical antiallodynic effect independent of whether it was injected into the neuropathic or contralateral hindpaw. In a test of mechanical nociception and hyperalgesia, dexmedetomidine markedly attenuated pain responses in all experimental groups, whereas MPV-2426 had a weak but significant pain attenuating effect only in neuropathic animals. In the tail flick test, both alpha(2)-adrenoceptor agonists had a significant antinociceptive effect. The pain attenuating effect of MPV-2426 was enhanced by pretreatment with 6-OHDA, except in a test of tactile allodynia. MPV-2426-induced modulation of spontaneous activity was not a general property of dorsal root fibers proximal to the injury. The results indicate that a chemical destruction of sympathetic postganglionic nerve fibers innervating the skin does not markedly influence cutaneous pain sensitivity nor is it critical for the alpha(2)-adrenoceptor agonist-induced attenuation of pain behavior in neuropathic or non-neuropathic animals. Chemical sympathectomy, independent of neuropathy, enhanced the pain attenuating effect by MPV-2426, probably due to a peripheral action, whereas in non-sympathectomized control and neuropathic animals peripheral mechanisms have only a minor, if any, role in the alpha(2)-adrenoceptor agonist-induced antinociception.
authors.affiliation: Department of Physiology, Institute of Biomedicine, University of Helsinki, Helsinki, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Wei
authors.forename: E
authors.initials: E
authors.lastname: Jyväsjärvi
authors.forename: S
authors.initials: S
authors.lastname: Niissalo
authors.forename: M
authors.initials: M
authors.lastname: Hukkanen
authors.forename: E
authors.initials: E
authors.lastname: Waris
authors.forename: Y T
authors.initials: YT
authors.lastname: Konttinen
authors.forename: A
authors.initials: A
authors.lastname: Pertovaara
mesh_terms: D058647:Adrenergic alpha-2 Receptor Agonists; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology; D007093:Imidazoles / Q000494:pharmacology; D007189:Indans / Q000494:pharmacology; D008026:Ligation; D008297:Male; D010147:Pain Measurement / Q000187:drug effects* / Q000379:methods / Q000706:statistics & numerical data; D051381:Rats; D017208:Rats, Wistar; D018341:Receptors, Adrenergic, alpha-2 / Q000502:physiology; D013127:Spinal Nerves / Q000293:injuries; D013563:Sympathectomy, Chemical / Q000379:methods / Q000706:statistics & numerical data; D017779:Sympathetic Fibers, Postganglionic / Q000187:drug effects / Q000502:physiology
chemical_list: D058647:Adrenergic alpha-2 Receptor Agonists; D000316:Adrenergic alpha-Agonists; D000700:Analgesics; D007093:Imidazoles; D007189:Indans; D018341:Receptors, Adrenergic, alpha-2; C400294:radolmidine