Application of recombinant fluorescent mammalian cells as a toxicity biosensor.
With respect to developing a more sensitive biosensor, a recombinant fluorescent Chinese Hamster Ovary cell line was used for the monitoring of various toxicants. Both cell lines, EFC-500 and KFC-A10, were able to detect toxicants sensitively. They were characterized with mitomycin C and gamma-ray as genotoxicants and bisphenol A, nonylphenol, ziram and methyl bromide as possible and known EDCs. When compared to each other, the response of KFC-A10 was generally more informative and sensitive. Compared to typical bacterial biosensor systems, these cell lines offered a sensitivity of 2- to 50-fold greater for the tested chemicals. Based on these results, the use of mammalian cells offers a sensitive biosensor system that is not only fast, cheap and reproducible but also capable of monitoring the endocrine-like characteristics of environmental toxicants.
Kim
E J
EJ
Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology and the Advanced Environmental Monitoring Research Center, Puk-gu, Korea.
Lee
Y
Y
Lee
J E
JE
Gu
M B
MB
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D016466:CHO Cells; D006224:Cricetinae; D004785:Environmental Pollutants / Q000633:toxicity*; D005453:Fluorescence; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004785:Environmental Pollutants