Effects of Insulin and cAMP on the High Density Lipoprotein Receptor in Cultured Human Arterial Smooth Muscle Cells.
Effects of insulin and cyclic AMP (cAMP) on HDL binding to cultured human arterial smooth muscles cells (SMC) were studied. Twenty-four hours after adding insulin to SMC cultured medium, the B(max) was decreased by 18% when compared with the control (P<0.05). On the other hand, twenty-four hours after adding db-cAMP to SMC cultured medium, the B(max) value was unchanged, but K(d) value was decreased 34% when compared with the control (P<0.001). The results suggested that insulin could induce the down regulation of the high density lipoprotein receptor and cAMP could promote HDL3 binding to SMC HDL receptor by increasing the affinity of the receptor in cultured human SMC.
Zhu
Hong
H
Apolipoprotein Research Unit, Institute of Biochemistry and Molecular Biology, West China University of Medical Sciences, Chengdu 610041, China.
Liu
Bing-Wen
BW