title: Patterns of chromosomal aberrations in urinary bladder tumours and adjacent urothelium.
abstract: Bladder cancer is often characterized by recurrent and multifocal growth, and tumours are frequently accompanied by precancerous alterations of the surrounding urothelium. These findings have led to the hypothesis that cells from areas of genetically aberrant but morphologically non-cancerous or even unremarkable mucosa may be the source of bladder carcinomas. Fluorescence in situ hybridization (FISH) was performed using ten probes targeting five different chromosomes that are known to be frequently altered in bladder cancer (centromere 1, 8, 9, 11, 17 and 1p36, 8p23, 9p21, 11q13, 17p13) on paraffin-embedded tissue sections of 11 superficial bladder cancers. Copy number changes of the tumours were compared to those in the urothelium adjacent to the tumour. Eleven of 11 (100%) tumours and eight of 11 (73%) samples of adjacent urothelium showed copy number changes of at least one chromosome. The occurrence of similar patterns of chromosomal aberrations in the tumours and their associated urothelium supports the hypothesis of a clonal relationship. It is concluded that FISH analysis targeting five different chromosomes is more sensitive than conventional histology for distinguishing between neoplastic and normal cells of the urothelium.
authors.affiliation: Gerhard-Domagk-Institute of Pathology and Department of Urology, University of Muenster, Muenster, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Steidl
authors.forename: Ronald
authors.initials: R
authors.lastname: Simon
authors.forename: Horst
authors.initials: H
authors.lastname: Bürger
authors.forename: Christian
authors.initials: C
authors.lastname: Brinkschmidt
authors.forename: Lothar
authors.initials: L
authors.lastname: Hertle
authors.forename: Werner
authors.initials: W
authors.lastname: Böcker
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Terpe
mesh_terms: D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology; D019459:Urothelium / Q000473:pathology
chemical_list: D015342:DNA Probes