Development of quantitative extraction method of amygdalin without enzymatic hydrolysis from tonin(Persicae Semen) by high performance liquid chromatography.
Tonin(Persicae Semen) is the herb medicine that contains amygdalin as a major ingredient. Amygdalin in water is decomposed into benzaldehyde, HCN, and glucose by emulsin, a hydrolysis enzyme in tonin. A useful and practical method for the optimum extraction condition of amygdalin without enzymatic hydrolysis is required. The extraction yield of amygdalin of natural formula tonin was 0.1% from crude powders, 1.4% from small pieces, 3.5% from half pieces and 2.4% from whole pieces. The extraction yield of amygdalin of outer shell-eliminated tonin was 0.3% from crude powders, 1.4% from small pieces, and 3.5% from half pieces and whole pieces respectively. The extraction yield of amygdalin was most high when using the size larger than half.
Hwang
Eun-Young
EY
Department of Oriental Pharmaceutical Sciences, Kyung Hee University, Seoul, Korea.
Lee
Sang-Soo
SS
Lee
Je-Hyun
JH
Hong
Seon-Pyo
SP
D000678:Amygdalin / Q000302:isolation & purification*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D000432:Methanol / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D014867:Water; D000678:Amygdalin; D000432:Methanol