In situ immunocytochemical detection of potyviral proteins in plant cells.
A method for in situ protein immunodetection using a peroxidase labeling system is described for detecting functional and structural proteins encoded by potato virus Y (Tunisian isolate) in plant tissues. Such Potyviruses are characterized by the accumulation of inclusion bodies containing viral encoded proteins other than coat protein. These proteins are functional at early stages of infection, making them easy to detect. Data are compared to those obtained by immunofluorescence techniques. Our technique can be used as a preliminary method for rapid detection of virus infection using antibodies directed against functional proteins.
Rouis
S
S
Center of Biotechnology, Sfax, Tunisia.
Ayadi
H
H
Bouaziz
S
S
Lakhoua
L
L
Gargouri
R
R
D000911:Antibodies, Monoclonal; D007150:Immunohistochemistry / Q000379:methods*; D009195:Peroxidase; D017800:Potyvirus / Q000378:metabolism*; D014026:Nicotiana / Q000378:metabolism* / Q000821:virology; D014764:Viral Proteins / Q000032:analysis* / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D014764:Viral Proteins; D009195:Peroxidase