The Application of Monoclonal Antibodies to the Imunoaffinity Purification of Human Ferritin.
There was difference between the reactivities of anti-human ferritin monoclonal antibodies, 6D6 and A-hF-C with liver and heart ferritins. 6D6 reacts with liver and heart ferritin with similar intensity, while A-hF-C reacts preferentially with liver ferritin. Two affinity gels were made with 6D6 and A-hF-C respectively, and used to purify ferritins from crude extracts of human liver and heart ferritins. The results have showed that it is a simple and time-saving method for the purification of ferritin with high purity and yield.
Zhang
De-Sheng
DS
Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
Cao
Hui-Ting
HT
Niu
Ching-I
CI
Zhang
Zu-Chuan
ZC