title: 1 L-myo-Inositol 1-Phosphate Synthase from Arabidopsis thaliana.
abstract: A recombinant phage containing an Arabidopsis thaliana cDNA sequence encoding a protein with 1L-myo-inositol 1-phosphate synthase (EC 5.5.1.4) activity has been isolated and used for transcriptional and translational studies. The identification of the recombinant phage relied on the observations that (a) the clone complements a mutation in the structural gene for 1L-myo-inositol 1-phosphate synthase in the yeast Saccharomyces cerevisiae, (b) the in vitro synthesized polypeptide enzymatically converts glucose 6-phosphate into inositol 1-phosphate, (c) in vitro transcription and translation of this cDNA sequence produces a polypeptide that is recognized by anti-yeast myo-inositol 1-phosphate synthase antiserum, and (d) inositol regulates the expression of the corresponding gene in Arabidopsis.
authors.affiliation: Department of Biological Sciences, University of Alabama, Tuscaloosa, Alabama 35487 (M.D.J.).
authors.forename: M. D.
authors.initials: MD
authors.lastname: Johnson
authors.forename: I. M.
authors.initials: IM
authors.lastname: Sussex