Interaction between the Anion Exchange Protein and the Glucose Transport Protein in Human Red Cell Membranes.
Cytochalasin B was used to inhibit the glucose transport through the glucose transport protein (GluT-1) in red cell membranes. It was observed that the anion transport was increased at the same time. The rate of anion transport was also increased when GluT-1 was bound with its substrate molecule-glucose. On the other hand, the experimental result on the relationship between glucose transport and Cl(-) concentration showed that K(m) was decreased and V(max) increased following the increase of Cl(-) concentration. There would be a bidirectional linkage between Band 3 (anion exchange protein) and GluT-1 in the intact red cell membranes. In other words, the conformation change of membrane protein could affect the function of other kinds of membrane proteins.
Zhang
Zhi-Hong
ZH
Department of Physiology and Biophysics, Fudan University, Shanghai 200433, China.
Peng
Feng
F
Li
Xiao-Quan
XQ
Zhou
Han-Qing
HQ
Hu
Xiao-Jian
XJ