title: A rapid PCR method of genotyping LDL receptor mutations in WHHL rabbits.
abstract: WHHL rabbits are a valuable model for the study of human familial hypercholesterolemia and atherosclerosis. To use this animal model, it is often necessary to confirm LDL receptor status in WHHL rabbits. Here, we described a simple and rapid PCR method to detect LDL mutations in WHHL rabbits.
authors.affiliation: Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Huijun
authors.initials: H
authors.lastname: Sun
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Usui
authors.forename: Masashi
authors.initials: M
authors.lastname: Shiomi
authors.forename: Teruo
authors.initials: T
authors.lastname: Watanabe
authors.forename: Jianglin
authors.initials: J
authors.lastname: Fan
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004195:Disease Models, Animal; D005838:Genotype; D006938:Hyperlipoproteinemia Type II / Q000235:genetics; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D011817:Rabbits; D011973:Receptors, LDL / Q000235:genetics*
chemical_list: D017931:DNA Primers; D011973:Receptors, LDL