title: Cloning and Expression of the Gene Coding for the Fusion Protein rhGM-CSF/LIF.
abstract: A fusion gene was constructed in a plasmid in which the coding regions of human GM_CSF and LIF cDNAs were connected by a synthetic linker sequence encoding a short peptide G-S-G-G-S through DNA recombinant techniques. It was then subcloned into the pBV220 expression vector, and expressed in E. coli after transformation and temperature induction. The expressed protein named as rhGM-LIF was confirmed by Western blot. After purification, the determination of activities showed that rhGM-LIF exhibited both GM-CSF and LIF activities.
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Zhang
authors.forename: Zhong-Liang
authors.initials: ZL
authors.lastname: Ge
authors.forename: Li-Ya
authors.initials: LY
authors.lastname: Sun
authors.forename: Qing-Ming
authors.initials: QM
authors.lastname: Ding
authors.forename: Qun-Wei
authors.initials: QW
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Cao
authors.forename: Li-Zhen
authors.initials: LZ
authors.lastname: Yuan