title: Differentiation of entamoeba histolytica/entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis.
abstract: To correlate a particular state of immunity with Entamoeba spp., we used colorimetric PCR to differentiate E. histolytica from E. dispar in individuals with amoebiasis and to associate its presence with the clinical profile, including humoral and cellular immune responses to E. histolytica. Our results showed high levels of antibody in acute amoebiasis and elevation of IL-4 production, a cytokine related to Th2 profile, associated with E. histolytica. In chronic amoebiasis, even with anti-E. histolytica seropositivity, intestinal symptoms were associated with E. dispar in all the cases, without differences in level of antibodies, BTI, CD4+/CD8+ ratio, INF-gamma, and IL-4. Among asymptomatic carriers, E. dispar was more frequently found; however, identification of E. histolytica in two asymptomatic carriers associated with high levels of INF-gamma, a cytokine related to Th1 profile, demonstrate the importance of making specific diagnosis of Entamoeba spp., to establish the clinical and epidemiological behavior in both intestinal and extra-intestinal amoebiasis.
authors.affiliation: Laboratorio de Parasitología, Centro de Investigación Biomédica de Oriente, IMSS, Puebla, México.
authors.forename: María Del Carmen
authors.initials: Mdel C
authors.lastname: Sánchez-Guillén
authors.forename: Ricardo
authors.initials: R
authors.lastname: Pérez-Fuentes
authors.forename: Hilda
authors.initials: H
authors.lastname: Salgado-Rosas
authors.forename: Alejandro
authors.initials: A
authors.lastname: Ruiz-Argüelles
authors.forename: John
authors.initials: J
authors.lastname: Ackers
authors.forename: Abdirashid
authors.initials: A
authors.lastname: Shire
authors.forename: Patricia
authors.initials: P
authors.lastname: Talamas-Rohana
mesh_terms: D000208:Acute Disease; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000917:Antibody Formation; D016516:CD4-CD8 Ratio; D002908:Chronic Disease; D016207:Cytokines / Q000097:blood; D004404:Dysentery, Amebic / Q000276:immunology / Q000503:physiopathology; D004747:Entamoeba / Q000145:classification* / Q000235:genetics / Q000276:immunology; D004748:Entamoeba histolytica / Q000145:classification* / Q000235:genetics / Q000276:immunology; D004749:Entamoebiasis / Q000175:diagnosis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008800:Mexico; D016133:Polymerase Chain Reaction / Q000379:methods; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
chemical_list: D000913:Antibodies, Protozoan; D016207:Cytokines; D015847:Interleukin-4; D007371:Interferon-gamma