Binding selectivity of conformationally restricted analogues of (-)-indolactam-V to the C1 domains of protein kinase C isozymes.
Two conformationally restricted analogues of (-)-indolactam-V (1) (cis and trans amides) were examined for their binding selectivity to the synthetic C1 peptides of all protein kinase C (PKC) isozymes. Although the binding constants of the cis amide-restricted analogue (2) were equal to those of 1, the trans amide-restricted analogue (3) bound significantly only to the novel PKC (delta, epsilon, eta, theta) isozymes.
Masuda
Akiko
A
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Japan.
Irie
Kazuhiro
K
Nakagawa
Yu
Y
Ohigashi
Hajime
H
D007211:Indoles / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D007769:Lactams / Q000378:metabolism*; D011487:Protein Conformation; D011493:Protein Kinase C / Q000378:metabolism*
D007211:Indoles; D007527:Isoenzymes; D007769:Lactams; C043969:indolactam V; D011493:Protein Kinase C