Kinetics and equilibrium in the immunoradiometric assay (IRMA) of thyroglobuline.
This paper studies the kinetics of the thyroglobuline reaction with its specific antibody immobilised on the inner wall of the reaction tube, and the subsequent binding of the immunocomplex formed with a second 125I-labelled antibody. These reactions are used in the immunoradiometric determination of thyroglobuline. Independent variables were analyte and labelled antibody, temperature, viscosity, and the medium's ionic strength. For the global process, mono-exponential kinetics were found to be dependent on the concentrations, such dependence fitting with the models discussed in the paper. Viscosity results clearly indicate its negative influence on the direct reaction rate. Ionic strength shows noticeable, but not too relevant, effects, which suggests that the variation caused by the glycerol addition is not due to the influence of the dielectric constant of the solutions used. The effect of temperature shows activation parameters similar to the viscous flow energy of water, which suggests diffusion control for the global process.
Garcia Gomez
J
J
Department of Physical Chemistry, Faculty of Pharmacy, Valencia University Hospital, Spain.
Moreno Frigols
J L
JL
D004058:Diffusion; D006801:Humans; D018796:Immunoconjugates; D015592:Immunoradiometric Assay; D007457:Iodine Radioisotopes; D007700:Kinetics; D008956:Models, Chemical; D009994:Osmolar Concentration; D013696:Temperature; D013954:Thyroglobulin / Q000032:analysis*; D014783:Viscosity
D018796:Immunoconjugates; D007457:Iodine Radioisotopes; D013954:Thyroglobulin