GHRH and IL1beta increase cytoplasmic Ca(2+) levels in cultured hypothalamic GABAergic neurons.
GHRH and IL1beta regulate sleep via the hypothalamus. However, actions of these substances on neurons are poorly understood. In this study, we found both GHRH (100 nM) and IL1beta (1.2 pM) acutely increased cytosolic Ca(2+) in 7.6 and 4.0% of cultured hypothalamic neurons tested, respectively, and 1.2% of neurons responded to both. The neurons that responded were mostly GABAergic (96, 81, and 100% for GHRH, IL1beta, and dual-responsive neurons, respectively).
De
Alok
A
Department of VCAPP, Program in Neuroscience, Washington State University, PO Box 646520, Pullman, WA 99164-6520, USA.
Churchill
Lynn
L
Obal
Ferenc
F
Simasko
Steven M
SM
Krueger
James M
JM
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D018929:Cell Culture Techniques; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism*; D013007:Growth Hormone-Releasing Hormone / Q000378:metabolism* / Q000494:pharmacology; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007375:Interleukin-1 / Q000378:metabolism* / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D007375:Interleukin-1; D005680:gamma-Aminobutyric Acid; D013007:Growth Hormone-Releasing Hormone; D002118:Calcium