title: Effects of osmolytes on unfolding of chicken liver fatty acid synthase.
abstract: Urea-induced aggregation of chicken liver fatty acid synthase [acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl- and enoyl-reducing and thioester-hydrolyzing), EC 2.3.1.85] was studied. The aggregation was facilitated at increased ionic strength. Methyl-beta-cyclodextrin and some osmolytes, such as glycerol, sucrose, proline, glycine, and heparin, could effectively prevent the aggregation, implying an artificial chaperone role of those substances during fatty acid synthase unfolding. The osmolytes also protected the enzyme from inactivation.
authors.affiliation: Department of Biological Science and Biotechnology, School of Life Science and Engineering, Tsinghua University, Beijing 100084, P. R. China.
authors.forename: Y-D
authors.initials: YD
authors.lastname: Park
authors.forename: B-N
authors.initials: BN
authors.lastname: Wu
authors.forename: W-X
authors.initials: WX
authors.lastname: Tian
authors.forename: H-M
authors.initials: HM
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology*; D002364:Caseins / Q000494:pharmacology*; D002645:Chickens; D003505:Cyclodextrins / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D064429:Fatty Acid Synthases / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D008055:Lipids / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D008110:Liver Extracts; D018832:Molecular Chaperones / Q000494:pharmacology; D009994:Osmolar Concentration; D014674:Plant Proteins, Dietary / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D014508:Urea / Q000494:pharmacology; D047392:beta-Cyclodextrins
chemical_list: D002241:Carbohydrates; D002364:Caseins; D003505:Cyclodextrins; D008055:Lipids; D008110:Liver Extracts; D018832:Molecular Chaperones; D014674:Plant Proteins, Dietary; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; C080652:Osmolite; D014508:Urea; D064429:Fatty Acid Synthases