Sox-4 is a positive regulator of Hep3B and HepG2 cells' apoptosis induced by prostaglandin (PG)A(2) and delta(12)-PGJ(2).
We reported earlier that expression of Sox-4 was found to be elevated during prostaglandin (PG) A(2) and delta(12)-PGJ(2) induced apoptosis in human hepatocarcinoma Hep3B cells. In this study, the role of Sox-4 was examined using human Hep3B and HepG2 cell lines. Sox-4 induction by several apoptotic inducer such as A23187 (Ca(2+) ionophore) and etoposide (topoisomerase II inhibitor) and Sox-4 transfection into the cells were able to induce apoptosis as observed by the cellular DNA fragmentation. Antisense oligonucleotide of Sox-4 inhibited the induction of Sox-4 expression and blocked the formation of DNA fragmentation by PGA(2) and delta(12)-PGJ(2) in Hep3B and HepG2 cells. Sox-4-induced apoptosis was accompanied with caspase-1 activation indicating that caspase cascade was involved in this apoptotic pathway. These results indicate that Sox-4 is involved in Hep3B and HepG2 cells apoptosis as an important apoptotic mediator.
Ahn
Sang-Gun
SG
Department of Biochemistry, College of Medicine, The Catholic University of Korea, Socho-Gu, Seoul 137-701, Korea.
Kim
Ho-Shik
HS
Jeong
Seong-Whan
SW
Kim
Bo-Eun
BE
Rhim
Hyangshuk
H
Shim
Jae-Yong
JY
Kim
Jin-Woo
JW
Lee
Jeong-Hwa
JH
Kim
In-Kyung
IK
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D020170:Caspase 1 / Q000378:metabolism; D061945:Caspase Inhibitors; D005047:Etoposide / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006609:High Mobility Group Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D009842:Oligopeptides / Q000494:pharmacology; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011454:Prostaglandins A / Q000494:pharmacology*; D055751:SOXC Transcription Factors; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
D061945:Caspase Inhibitors; D006609:High Mobility Group Proteins; D009842:Oligopeptides; D011454:Prostaglandins A; C083431:SOX4 protein, human; D055751:SOXC Transcription Factors; D015534:Trans-Activators; C096429:L 709049; D000001:Calcimycin; C037112:9-deoxy-delta-9-prostaglandin D2; D005047:Etoposide; D020170:Caspase 1; C100008:prostaglandin A2; D015230:Prostaglandin D2