title: Eotaxin gene single nucleotide polymorphisms in the promoter and exon regions are not associated with susceptibility to atopic dermatitis, but two of them in the promoter region are associated with serum IgE levels in patients with atopic dermatitis.
abstract: Eotaxin is believed to play an important role in atopic dermatitis (AD) as a potent chemoattractant and activator of eosinophils and Th2 lymphocytes. The eotaxin gene is located at chromosome 17q21.1-q21.2, and linkage findings of AD on chromosome 17 were reported. Recently we have identified single nucleotide polymorphisms (SNPs) of eotaxin gene (-426C > T, -384A > G, 67G > A). To learn whether eotaxin gene SNPs are associated with susceptibility to AD or phenotypes of AD, we investigated the genotype frequencies at each SNP of the gene in AD patients and in controls. We examined 140 Japanese AD patients and 140 healthy Japanese individuals. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism method. No significant difference was observed in allele or genotype frequencies of any SNP between AD patients and controls. Serum immunoglobulin E (IgE) levels were significantly lower in CT and TT genotype than in CC (P = 0.038) in -426C > T SNP, and lower in GG than in AA and AG with borderline significance (P = 0.053) in -384A > G SNP in AD patients. Eotaxin gene SNPs in the promoter and exon regions are not associated with susceptibility to AD, but two of them in the promoter region are associated with phenotype of AD.
authors.affiliation: Department of Dermatology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan. tsunemiy-der@h.u-tokyo.ac.jp
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Tsunemi
authors.forename: Hidehisa
authors.initials: H
authors.lastname: Saeki
authors.forename: Koichiro
authors.initials: K
authors.lastname: Nakamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Sekiya
authors.forename: Koichi
authors.initials: K
authors.lastname: Hirai
authors.forename: Hideki
authors.initials: H
authors.lastname: Fujita
authors.forename: Noriko
authors.initials: N
authors.lastname: Asano
authors.forename: Yuka
authors.initials: Y
authors.lastname: Tanida
authors.forename: Takashi
authors.initials: T
authors.lastname: Kakinuma
authors.forename: Motoshi
authors.initials: M
authors.lastname: Wakugawa
authors.forename: Hideshi
authors.initials: H
authors.lastname: Torii
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Tamaki
mesh_terms: D000293:Adolescent; D000328:Adult; D054413:Chemokine CCL11; D019742:Chemokines, CC / Q000235:genetics*; D002648:Child; D003876:Dermatitis, Atopic / Q000097:blood* / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: C515456:CCL11 protein, human; D054413:Chemokine CCL11; D019742:Chemokines, CC; D007073:Immunoglobulin E