Transcriptional regulation of the human high affinity IgE receptor alpha-chain gene.
Transcriptional regulation of the gene encoding human high affinity IgE receptor (Fc epsilon RI) alpha-chain was analyzed. Previously, we reported that GATA-1 and Elf-1 recognition sites were necessary for cell type-specific activation of the alpha-chain gene promoter. More detailed analysis revealed that other transcription factors bound the regions close to the Elf-1 recognition site and there was a more complex mechanism for the regulation of the promoter activity. On the other hand, during a course of studies to find cis-elements over this gene, CAGCTG sequence in the first intron was revealed to serve as an enhancer. A complex composed of USF1 and USF2 activated the human alpha-chain gene expression via this intronic element. Furthermore, we found two novel exons at 18.4 and 12.6kb upstream from the reported first exon and discovered an additional distal promoter.
Takahashi
Kyoko
K
Department of Molecular Cell Immunology and Allergology, Advanced Medical Research Center, Nihon University School of Medicine, 30-1 Oyaguchi-kamimachi, Itabashi-ku, 173-8610, Tokyo, Japan.
Nishiyama
Chiharu
C
Ra
Chisei
C
D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017455:Receptors, IgE / Q000096:biosynthesis / Q000235:genetics*; D015533:Transcriptional Activation
C475664:FCER1A protein, human; D017455:Receptors, IgE