title: Establishment and characterization of a new osteogenic cell line (MOS-J) from a spontaneous C57BL/6J mouse osteosarcoma.
abstract: In this paper we describe the establishment and characterization of a transplantable cell line derived from a spontaneously occurring chondroblastic osteosarcoma in a C57BL/6J mouse. The tumor line, MOS-J, forms solid tumors when injected intramuscularly into immunocompetent syngeneic hosts, mimicking endochondral bone development. These transplantable tumors have the capacity to destroy and invade existing bone and invade vessels in close proximity to the tumor. In culture, MOS-J cells form layers of pleomorphic cells with high mitotic activity. These cells have marked alkaline phosphatase activity and form calcified foci in vitro that stain with alizarin red. MOS-J cells also promote osteoclast development in vitro from normal bone marrow cells. These characteristics indicate the potential utility of the MOS-J osteosarcoma cell line as a model for studies of human osteosarcoma and bone biology.
authors.affiliation: Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Joliat
authors.forename: Syuji
authors.initials: S
authors.lastname: Umeda
authors.forename: Bonnie L
authors.initials: BL
authors.lastname: Lyons
authors.forename: Michael A
authors.initials: MA
authors.lastname: Lynes
authors.forename: Leonard D
authors.initials: LD
authors.lastname: Shultz
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones; D001859:Bone Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D004195:Disease Models, Animal; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D010010:Osteoclasts / Q000166:cytology; D012516:Osteosarcoma / Q000000981:diagnostic imaging / Q000473:pathology*; D011859:Radiography; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured / Q000637:transplantation / Q000648:ultrastructure
chemical_list: D000880:Anthraquinones; C010078:alizarin; D000469:Alkaline Phosphatase