[An analysis of DNA content in 405 cases of human tumor by flow cytometry].
This study was intended to evaluate the relationship between the DNA content of cell cycle and the histology in human tumors. We detected the DNA content in 405 cases of fresh human tumor tissue by means of flow cytometry and observed the histology of tumor with light microscopy. The occurrences of aneuploidy in 22 cases of benign tumor and 383 cases of malignant tumor were 27% and 52% respectively. There were differences in aneuploidy in different histologic types of tumor. The aneuploidy in adenocarcinoma was about 50%, that in sarcoma was more than 37%, and that in squamous carcinoma less than 17%. S > or = 10, G2/m > or = 10 or S > or = 20, G2/m > or = 5 were present in malignant tumors, but were not found in benign tumors. The results suggest that there is obvious difference in the occurrences of aneuploidy in benign and malignant tumors and in different histologic type of tumor(P < 0.05). Benign and malignant tumor may be distinguished when SPF and G2/m reach a higher level (P < 0.01).
Lei
S
S
Tumor Biotherapy Research Center, First Affiliated Hospital, WCUMS, Chengdu 610041.
Wei
Y
Y
Mao
Y
Y
Hang
Z
Z
Zhao
X
X
Yan
L
L
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D007414:Intestinal Neoplasms / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm