RPD3 is required for the inactivation of yeast ribosomal DNA genes in stationary phase.
rRNA transcription in Saccharomyces cerevisiae is performed by RNA polymerase I and regulated by changes in growth conditions. During log phase, approximately 50% of the ribosomal DNA (rDNA) genes in each cell are transcribed and maintained in an open, psoralen-accessible conformation. During stationary phase, the percentage of open rDNA genes is greatly reduced. In this study we found that the Rpd3 histone deacetylase was required to inactivate (close) individual rDNA genes as cells entered stationary phase. Even though approximately 50% of the rDNA genes remained open during stationary phase in rpd3Delta mutants, overall rRNA synthesis was still reduced. Using electron microscopy of Miller chromatin spreads, we found that the number of RNA polymerases transcribing each open gene in the rpd3Delta mutant was significantly reduced when cells grew past log phase. Bulk levels of histone H3 and H4 acetylation were reduced during stationary phase in an RPD3-dependent manner. However, histone H3 and H4 acetylation was not significantly altered at the rDNA locus in an rpd3Delta mutant. Rpd3 therefore regulates the number of open rDNA repeats.
Sandmeier
Joseph J
JJ
Department of Biochemistry and Molecular Genetics and Department of Microbiology, University of Virginia Health System, Charlottesville, VA 22908, USA.
French
Sarah
S
Osheim
Yvonne
Y
Cheung
Wang L
WL
Gallo
Christopher M
CM
Beyer
Ann L
AL
Smith
Jeffrey S
JS
D000107:Acetylation; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D004275:DNA, Ribosomal / Q000187:drug effects / Q000235:genetics*; D005363:Ficusin / Q000494:pharmacology; D005800:Genes, Fungal; D006655:Histone Deacetylases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D009154:Mutation; D012318:RNA Polymerase I / Q000378:metabolism; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014157:Transcription Factors; D014158:Transcription, Genetic
D002843:Chromatin; D003432:Cross-Linking Reagents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D006657:Histones; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D012318:RNA Polymerase I; C071665:RPD3 protein, S cerevisiae; D006655:Histone Deacetylases; D005363:Ficusin