[Experimental study of tendon allograft].
A series of immunologic tests, including lymphocyte cell toxicity test, as well as lymphocyte transformation test, macrophage function test, histological examination, and biomechanical measurement were carried out in guinea pigs to compare the effect of deep freezing(Group I) with deep freeze drying(Group II) on tendon allografts. The results revealed that the lymphocyte mortality, lymphocytic-transformed rate and phagocytizing rate of the macrophages in Group I and Group II were lower than those in non-processed allograft group (Group III) and had no statistical difference among Group I, Group II and Group III. As compared with the non-processed allograft, the freezed and freeze-dried allografts showed ameliorated immunologic reactions, mild morphological lesion and better mechanical strength after transplantation. The results suggest that deep freezing and deep freeze-drying processing are valid measures in tendon allograft treatment.
Wang
X Y
XY
Department of Ortheopedics, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Li
K H
KH
Wang
W G
WG
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D003601:Cytotoxicity Tests, Immunologic; D006168:Guinea Pigs; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D011897:Random Allocation; D013710:Tendons / Q000637:transplantation*