title: A family of novel DNA sequencing instruments based on single-photon detection.
abstract: We have developed a family of high-performance capillary DNA sequencing instruments based on a novel multicolor fluorescent detection technology. This technology is based on two technical innovations: the multilaser excitation of fluorescence of labeled DNA fragments and the "color-blind" single-photon detection of modulated fluorescence. Our machines employ modern digital and broadband techniques that are essential for achieving superior instrument performance. We discuss the design and testing results for several versions of the automated single lane DNA sequencers, as well as our approach to scaling up to multilane instruments.
authors.affiliation: Department of Electrical and Computer Engineering, State University of New York-Stony Brook, Stony Brook, NY 11794-2350, USA.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Alaverdian
authors.forename: Sinok
authors.initials: S
authors.lastname: Alaverdian
authors.forename: Olga
authors.initials: O
authors.lastname: Bilenko
authors.forename: Iouri
authors.initials: I
authors.lastname: Bogdanov
authors.forename: Elena
authors.initials: E
authors.lastname: Filippova
authors.forename: Dmitry
authors.initials: D
authors.lastname: Gavrilov
authors.forename: Boris
authors.initials: B
authors.lastname: Gorbovitski
authors.forename: Michael
authors.initials: M
authors.lastname: Gouzman
authors.forename: Georgy
authors.initials: G
authors.lastname: Gudkov
authors.forename: Sergey
authors.initials: S
authors.lastname: Domratchev
authors.forename: Olga
authors.initials: O
authors.lastname: Kosobokova
authors.forename: Nadia
authors.initials: N
authors.lastname: Lifshitz
authors.forename: Serge
authors.initials: S
authors.lastname: Luryi
authors.forename: Victor
authors.initials: V
authors.lastname: Ruskovoloshin
authors.forename: Andrew
authors.initials: A
authors.lastname: Stepoukhovitch
authors.forename: Marina
authors.initials: M
authors.lastname: Tcherevishnick
authors.forename: Georgy
authors.initials: G
authors.lastname: Tyshko
authors.forename: Vera
authors.initials: V
authors.lastname: Gorfinkel
mesh_terms: D003116:Color; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D005456:Fluorescent Dyes; D007834:Lasers; D017785:Photons; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation*
chemical_list: D005456:Fluorescent Dyes