title: Advantages of continuous over batch reactors for the kinetic analysis of enzymes inhibited by an unknown substrate impurity.
abstract: A new experimental technique, employing a continuous stirred-tank reactor, for studying enzyme kinetics in the presence of inhibitor-contaminated substrate is described. The proposed method is simulated mathematically for competitive, uncompetitive, and mixed-type noncompetitive inhibition. The step-by-step experimental procedure is described, as is the necessary data analysis for determining the kinetic parameters. Differences in system response for enzyme inhibition by excess substrate and by an impurity are illustrated, and a stability analysis of the system is performed.
authors.affiliation: Dipartimento di Chimica, Ingegneria Chimica e Materiali, Università di L'Aquila, 67040 Monteluco di Roio, AQ, Italy. gallifuo@ing.univaq.it
authors.forename: Alberto
authors.initials: A
authors.lastname: Gallifuoco
authors.forename: Francesco
authors.initials: F
authors.lastname: Alfani
authors.forename: Maria
authors.initials: M
authors.lastname: Cantarella
mesh_terms: D019149:Bioreactors; D003198:Computer Simulation; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D008954:Models, Biological; D008956:Models, Chemical; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
chemical_list: D004791:Enzyme Inhibitors; D004798:Enzymes