Affinity Purification of Hepatitis B Virus Surface Antigen Containing PreS1 Region.
The large protein of hepatitis B virus surface antigen (LHBs) contained an attachment site of HBV to liver cells and the antibodies to preS1 were virus-neutralizing. Therefore, vaccines containing preS1 would be more protective. However, One of the key problems in the preparation of gene-expressed proteins was the purification of the products. We have established a method of immuno-affinity chromatography with the anti-HBsAg-preS1 monoclonal antibody. Using this technique, We have successfully purified gene-expressed fusion protein of hepatitis B virus surface antigen containing preS1 (21-47). Results showed that this method was simple, effective and specific as compared to other methods, and might be useful in the future.
Yang
Hai-Lin
HL
Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
Jin
Yi
Y
Cao
Hui-Ting
HT
Xu
Xie
X
Li
Guang-Di
GD
Wang
Yuan
Y
Zhang
Zu-Chuan
ZC