Efficient transduction of liver and muscle after in utero injection of lentiviral vectors with different pseudotypes.
In this study we investigate the efficacy of lentiviral vectors of different pseudotypes for gene transfer to tissues of the preimmune fetus. BALB/c fetuses at 14-15 days' gestation received lentiviral vectors carrying the transgene lacZ under the control of the human cytomegalovirus (CMV) promoter by intramuscular (i.m.) or intrahepatic (i.h.) injection. We pseudotyped the lentiviral vectors with vesicular stomatitis virus (VSV-G), with Mokola virus, or with Ebola virus envelope glycoproteins. We harvested the pups at time points between 5 days and 9 months following injection and performed a detailed histologic assessment. The efficiency and distribution of transduction after in utero administration was highly dependent upon the route of administration and the pseudotype of vector used. Biodistribution studies showed widespread distribution of vector sequences in multiple tissues, albeit at very low levels, and transduced cells were found in significant numbers only in liver, heart, and muscle. Overall, VSV-G was the most efficient in transducing hepatocytes, whereas Mokola and Ebola were more efficient in transducing myocytes. Transduction of cardiomyocytes was observed after both i.m. and i.h. injection of all three vectors. Our findings of long-term transduction of skeletal myocytes and cardiomyocytes after in utero administration suggest a novel strategy for the treatment of congenital muscular dystrophies.
MacKenzie
Tippi C
TC
Children's Institute for Surgical Science, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA.
Kobinger
Gary P
GP
Kootstra
Neeltje A
NA
Radu
Antoneta
A
Sena-Esteves
Miguel
M
Bouchard
Sarah
S
Wilson
James M
JM
Verma
Inder M
IM
Flake
Alan W
AW
D000818:Animals; D029043:Ebolavirus / Q000235:genetics; D005260:Female; D005333:Fetus / Q000187:drug effects*; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000502:physiology* / Q000633:toxicity; D006321:Heart / Q000187:drug effects; D016086:Lentivirus / Q000235:genetics*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D009132:Muscles / Q000166:cytology / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology; D032383:Myocytes, Cardiac / Q000187:drug effects; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D014161:Transduction, Genetic