title: Analysis of reaction products of cocaine and hydrogen peroxide by high-performance liquid chromatography/mass spectrometry.
abstract: The change of chemical structure of cocaine in the presence of hydrogen peroxide, a main component of hair dye and decolorant treatments, was studied. High-performance liquid chromatography/mass spectrometry (LC/MS) was used for the separation and identification of cocaine derivatives. After a mixture of cocaine and hydrogen peroxide solutions was incubated at 39 degrees C (this temperature is commonly used when the hair is treated with hair dye or decolorant) for 24 h, six reaction products were detected by LC/MS. Two of them were ecgonine methyl ester and benzoylecgonine, which are metabolites of cocaine. The other reaction products were assumed to be ortho-, meta- and para-hydroxycocaines and dihydroxycocaine, in each of which the benzene ring was hydroxylated by the reaction. These five reaction products (except for dihydroxycocaine) were found immediately after mixing cocaine and hydrogen peroxide. Therefore, the above reaction products might be present in the hair of cocaine users that had treated their hair with hair dye or decolorant.
authors.affiliation: Forensic Science Laboratory, Ishikawa Prefectural Police Headquarters, 2-1-1 Hirosaka, Kanazawa 920-8553, Japan.
authors.forename: Seishi
authors.initials: S
authors.lastname: Tanaka
authors.forename: Reiko
authors.initials: R
authors.lastname: Iio
authors.forename: Satoshi
authors.initials: S
authors.lastname: Chinaka
authors.forename: Nariaki
authors.initials: N
authors.lastname: Takayama
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003042:Cocaine / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D006861:Hydrogen Peroxide; D003042:Cocaine