Patterning DNA on microm scale on mica.
Double-stranded DNA molecules were patterned by selective adsorption to aminosilane patterns on mica surfaces. Line patterns with 10 microm spacing were made by photolithography and transferred to a polymer stamp. The stamp was then used for applying aminosilane molecules by microcontact-printing technique on mica substrates. We applied DNA in Tris-EDTA (TE) buffer solution on the patterned substrate, and incubated it for 5 min at room temperature. The sample was then rinsed with pure water, and dried with nitrogen gas. Tapping mode force microscopy showed that DNA was adsorbed selectively on the aminosilanized parts of the mica substrate. We also tried to bridge two aluminum electrodes with DNA using AC electrophoresis.
Fujita
M
M
National Institute of Advanced Industrial Science and Technology, Nanotechnology Research Institute, Tsukuba, Japan.
Mizutani
W
W
Gad
M
M
Shigekawa
H
H
Tokumoto
H
H
D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D004566:Electrodes; D004586:Electrophoresis / Q000379:methods; D018625:Microscopy, Atomic Force / Q000379:methods*; D012821:Silanes / Q000737:chemistry
D000538:Aluminum Silicates; D012821:Silanes; D004247:DNA; C011934:mica