Influence of inoculum type, inorganic salt and nitrogen to carbon ratio on sclerotium formation and carotenoid production in surface culture of Penicillium sp. PT95.
This study examined the respective effect of inoculum type, inorganic salt and nitrogen to carbon ratio on sclerotium formation and carotenoid production in surface culture of Penicillium sp. PT95. Neither the spore inoculum nor the mycelial pellet inoculum could result in the formation of sclerotium on a modified Czapek agar medium after incubation of 28 days, whereas the inoculum in the form of sclerotium caused the formation of numerous orange, sand-shaped sclerotia after incubation of 14 days. Among four inorganic salts tested, K(2)HPO(4) was more essential to the sclerotium formation and carotenoid production of strain PT95 as compared to KCl, MgSO(4) or FeSO(4). It was also shown that the combination of K(2)HPO(4), KCl and MgSO(4) could produce the best positive cooperation and give the highest sclerotia biomass (782 mg/plate) and carotenoid content in sclerotium (420 microg/g of dry sclerotia) as well as pigment yield (328 microg/plate). The medium containing 0.24 approximately 0.48 g/l sodium nitrate-nitrogen was effective to both the sclerotium formation and carotenoid production of strain PT95 when available maltose-carbon concentrations were at 5.26 approximately 21.05 g/l. The optimal N:C ratio was found to be 1:25.
Han
Jian-Rong
JR
Department of Life Science, Shanxi University, Taiyuan 030006, People's Republic of China. hjr@sxu.edu.cn
Xu
Jun
J
Zhou
Xiao-Mei
XM
D018533:Biomass; D002244:Carbon / Q000378:metabolism; D002338:Carotenoids / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D008320:Maltose / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010407:Penicillium / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D012988:Soil Microbiology
D003470:Culture Media; D009566:Nitrates; D010710:Phosphates; D002338:Carotenoids; D008320:Maltose; D002244:Carbon; C031618:sodium nitrate; D009584:Nitrogen