title: Immunophenotypic clustering of myelodysplastic syndromes.
abstract: Myelodysplastic syndromes (MDSs) are heterogeneous diseases of bone marrow (BM) cell precursors for which immunophenotypic characterization is still considered irrelevant despite the accuracy and sensitivity of flow cytometry techniques. The aim of this study was to determine whether immunophenotypic abnormalities could be defined in MDSs and could correlate with the French-American-British classification and cytogenetics. Analysis was performed on 275 BM samples (207 MDS patients, 68 controls) and 25 control blood samples. Immunophenotyping was based on a primary gating of blast cells, monocytes, and granulocytes according to CD45 antigen expression and side scatter light diffraction. Immunophenotypic hierarchical clustering was performed to analyze the results. The data obtained show that (1) immunophenotypic clustering partly discriminates patients with refractory anemia with excess blasts/refractory anemia with excess blasts in transformation (RAEB/RAEB-T), chronic myelomonocytic leukemia (CMML), and refractory anemia/refractory anemia with ring sideroblasts (RA/RARS) for CD45(lo) blast cells and patients with RA/CMML, RARS, and RAEB/RAEB-T for CD45(hi)/side scatter(hi) (SS(hi)) granulocytes; (2) the most discriminating markers were CD16, CD34, CD36, CD38, CD71, and HLA-DR for blast cells and CD11b, CD13, CD33, CD36, CD38, CD71, and HLA-DR for CD45(hi)/SS(hi) granulocytes; (3) clusters related to CD34 expression were associated with high levels of blast cells on BM smear; (4) clusters related to high levels of CD36 expression on CD45(lo) blast cells and CD45(hi)/SS(hi) granulocytes were associated with a poor International Prognosis Scoring System score; and (5) high levels of CD71 expression on CD45(hi)/SS(hi) granulocytes were associated with the RARS category. These results show a close relationship between immunophenotypic abnormalities and BM dysplasia and suggest that flow cytometry could be a future tool for the characterization of MDSs.
authors.affiliation: Service d'Hématologie Biologique, CHU Bocage, Dijon, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Maynadié
authors.forename: Françoise
authors.initials: F
authors.lastname: Picard
authors.forename: Bernard
authors.initials: B
authors.lastname: Husson
authors.forename: Bernard
authors.initials: B
authors.lastname: Chatelain
authors.forename: Yvan
authors.initials: Y
authors.lastname: Cornet
authors.forename: Geneviève
authors.initials: G
authors.lastname: Le Roux
authors.forename: Lydia
authors.initials: L
authors.lastname: Campos
authors.forename: Alex
authors.initials: A
authors.lastname: Dromelet
authors.forename: Pascalle
authors.initials: P
authors.lastname: Lepelley
authors.forename: Hélène
authors.initials: H
authors.lastname: Jouault
authors.forename: Michèle
authors.initials: M
authors.lastname: Imbert
authors.forename: Michelle
authors.initials: M
authors.lastname: Rosenwadj
authors.forename: Véronique
authors.initials: V
authors.lastname: Vergé
authors.forename: Philippe
authors.initials: P
authors.lastname: Bissières
authors.forename: Martine
authors.initials: M
authors.lastname: Raphaël
authors.forename: Marie Christine
authors.initials: MC
authors.lastname: Béné
authors.forename: Jean
authors.initials: J
authors.lastname: Feuillard
mesh_terms: D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology; D018952:Antigens, CD34 / Q000032:analysis; D001854:Bone Marrow Cells / Q000276:immunology / Q000473:pathology; D016000:Cluster Analysis; D006801:Humans; D016130:Immunophenotyping / Q000145:classification* / Q000379:methods; D017493:Leukocyte Common Antigens / Q000032:analysis; D009190:Myelodysplastic Syndromes / Q000145:classification / Q000276:immunology* / Q000473:pathology
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D017493:Leukocyte Common Antigens