Performance of an ultraviolet light-emitting diode-induced fluorescence detector in capillary electrophoresis.
The performance of a fluorescence detector in capillary electrophoresis (CE) using a light-emitting diode (LED) as excitation source is reported. An ultraviolet LED pulsed at a repetition rate of 500 Hz, combined with a time-discrimination and averaging acquisition system, was used. Limits of detection of 3 and 18 fmoles (at a signal-to-noise ratio equal to 3) were achieved for fluorescamine-derivatized bradykinin and lysine, respectively. This system exhibited a linear response for a concentration range between 54 and 417 microM for derivatized lysine, and between 1.81 and 23.58 microM for derivatized bradykinin. This detection system showed to be very convenient for routine analytical applications.
Hillebrand
Sandro
S
Centro de Biotecnologia do Estado do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Schoffen
Júlio Ricardo
JR
Mandaji
Marcos
M
Termignoni
Carlos
C
Grieneisen
Hans Peter Henrik
HP
Kist
Tarso Benigno Ledur
TB
D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000032:analysis; D001920:Bradykinin / Q000031:analogs & derivatives / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D005450:Fluorescamine; D007202:Indicators and Reagents; D008239:Lysine / Q000031:analogs & derivatives / Q000032:analysis; D010447:Peptide Hydrolases / Q000032:analysis; D012694:Serine / Q000031:analogs & derivatives / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods* / Q000706:statistics & numerical data; D014466:Ultraviolet Rays
D007202:Indicators and Reagents; D001224:Aspartic Acid; D005450:Fluorescamine; D012694:Serine; D010447:Peptide Hydrolases; C035855:keratinase; D008239:Lysine; D001920:Bradykinin