title: Group IB secretory phospholipase A(2)induces cell death in the cultured cortical neurons: a possible involvement of its binding sites.
abstract: In primary cultures of rat cortical neurons, group IB secretory phospholipase A(2) (sPLA(2)-IB) induced cell death. In rat cortical membranes, there were high affinity binding sites of [125I]sPLA(2)-IB. The high-affinity binding sites were decreased by sPLA(2)-IB and anti-sPLA(2) receptor immunoglobulin G (anti-sPLA(2)R IgG). Furthermore, anti-sPLA(2)R IgG caused neuronal cell death in a concentration-dependent manner. The present study suggests that sPLA(2)-IB induces neuronal cell death via its high-affinity binding sites.
authors.affiliation: Discovery Research Laboratories, Shionogi and Co Ltd, 12-4 Sagisu 5-Chome, Fukushima-ku, Osaka 553-0002, Japan. tatsurou.yagami@shionogi.co.jp
authors.forename: Tatsurou
authors.initials: T
authors.lastname: Yagami
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ueda
authors.forename: Kenji
authors.initials: K
authors.lastname: Asakura
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hata
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kuroda
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sakaeda
authors.forename: Jyunji
authors.initials: J
authors.lastname: Kishino
authors.forename: G
authors.initials: G
authors.lastname: Sakaguchi
authors.forename: Naohiro
authors.initials: N
authors.lastname: Itoh
authors.forename: Yozo
authors.initials: Y
authors.lastname: Hori
mesh_terms: D000818:Animals; D001665:Binding Sites; D018929:Cell Culture Techniques; D016923:Cell Death / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D010741:Phospholipases A / Q000009:adverse effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010741:Phospholipases A