title: Polyamine Accumulation and Near Loss of Morphogenesis in Long-Term Callus Cultures of Rice (Restoration of Plant Regeneration by Manipulation of Cellular Polyamine Levels).
abstract: We have shown (S. Bajaj and M.V. Rajam [1995] Plant Cell Rep 14: 717-720) that a significant reduction in morphogenetic potential occurs in callus cultures of rice (Oryza sativa L. cv TN-1) (up to 1 year old), and that plant regeneration could be improved in such cultures with spermidine treatment. We now show a near loss in plant regeneration capacity, concomitant with massive polyamine accumulation (primarily the diamine putrescine), due to the increase in arginine decarboxylase activity and an altered putrescine-to-spermidine ratio in 20- and 36-month-old rice callus cultures. The blockage of polyamine accumulation due to the reduction in arginine decarboxylase activity by a putrescine synthesis inhibitor, [alpha]-difluoromethylarginine, completely restored plant regeneration capacity in these long-term cultures. Additionally, spermidine treatment of long-term cultures caused an increase in cellular spermidine content and a reduction in putrescine content and arginine decarboxylase activity, leading to an adjustment in putrescine-to-spermidine ratio and the restoration of plant regeneration ability.
authors.affiliation: Department of Genetics, University of Delhi, South Campus, Benito Juarez Road, New Delhi 110021, India.
authors.forename: S.
authors.initials: S
authors.lastname: Bajaj
authors.forename: M. V.
authors.initials: MV
authors.lastname: Rajam