Cutting edge: quantitative imaging of raft accumulation in the immunological synapse.
Although the accumulation of lipid rafts at the immunological synapse is now well accepted, the degree of the accumulation, the localization within the fine structure of the immunological synapse, and the region from which lipid rafts are recruited have not been defined. In this work we show that lipid rafts preferentially accumulate in the central zone of the immunological synapse, the central supramolecular activation complex (C-SMAC). However, quantitative analyses indicate that the level of recruitment of lipid rafts to the C-SMAC is relatively small and suggests that rearrangement of lipid rafts from the peripheral zone of the synapse into the C-SMAC can account for this accumulation. We also assessed the effects of CD28 deficiency on lipid raft recruitment to the immunological synapse. The accumulation of lipid occurred independently of the CD28/B7 system and was not measurably altered by CD28.
Burack
W Richard
WR
Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Lee
Kyeong-Hee
KH
Holdorf
Amy D
AD
Dustin
Michael L
ML
Shaw
Andrey S
AS
D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D018106:CD28 Antigens / Q000235:genetics; D002450:Cell Communication / Q000276:immunology*; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D003505:Cyclodextrins / Q000494:pharmacology; D005677:G(M1) Ganglioside / Q000378:metabolism; D008213:Lymphocyte Activation; D021962:Membrane Microdomains / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D047392:beta-Cyclodextrins
D018106:CD28 Antigens; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D005677:G(M1) Ganglioside; D002772:Cholera Toxin; D002784:Cholesterol