title: Application of the yeast two-hybrid system in molecular gerontology.
abstract: Most -- if not all -- proteins are bound to interact with other proteins to exert their function, and thus the identification of the interaction partners of a protein is vital in proteomics. The yeast two-hybrid system is a popular and effective tool for studying protein-protein interactions. Although the advantages of the system are manifold, it also has certain drawbacks and limitations. The two-hybrid system has been shown to be extremely useful for placing a protein of unknown function within a functional context, thereby providing information about a putative role of the uncharacterised protein. This concept has also been successfully applied in molecular gerontology.
authors.affiliation: Institute of Molecular and Structural Biology, Aarhus University, Gustav Wieds Vej 10C, DK-8000 Arhus C, Denmark. crk@imsb.au.dk
authors.forename: Charlotte Rohde
authors.initials: CR
authors.lastname: Knudsen
authors.forename: Mandana
authors.initials: M
authors.lastname: Jadidi
authors.forename: Irene
authors.initials: I
authors.lastname: Friis
authors.forename: Francisco
authors.initials: F
authors.lastname: Mansilla
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D005853:Geriatrics; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012106:Research; D020798:Two-Hybrid System Techniques
chemical_list: D011506:Proteins