Utilization of Lactobacillus sp. for steroid glycoalkaloids degradation by lactic acid fermentation.
The degradation fo steroid glycoalkaloids (SGAs) has been studied in model solutions. The number of colony forming units (CFU) was determined using a nondirect (cultivation) method during all stages of fermentation. The changes in SGAs content were observed by HPLC on the Supelcosil LC-NH2 column. The changes in alpha-tomatine concentration added to fermented Lactobacillus MRS broth have been studied. A mathematical model of steroid glycoalkaloids degradation during lactic fermentation was proposed. The mathematical model was based on the experimental data of SGA and glucose concentration and should be used for study and prediction of SGA concentration changes of fermented samples. The ratio of SGA degradation rate by fermentation and by lactic acid hydrolysis was calculated. The experimental data evaluated by proposed mathematical model for the selected strain of Lactobacillus plantarum 976H show real feasibility for SGA degradation by lactic acid fermentation.
Veselá
Maria
M
Faculty of Chemistry, Brno University of Technology, Brno, Czech Republic. vesela@fch.vutbr.cz
Drdák
M
M
Simon
P
P
Veselý
M
M
D000470:Alkaloids / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015169:Colony Count, Microbial; D003470:Culture Media; D005285:Fermentation; D006027:Glycosides / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism*; D008433:Mathematics; D008956:Models, Chemical; D013256:Steroids / Q000378:metabolism; D014053:Tomatine / Q000378:metabolism
D000470:Alkaloids; D003470:Culture Media; D006027:Glycosides; D013256:Steroids; D014053:Tomatine; D019344:Lactic Acid