title: The cytoplasmic tail peptide sequence of membrane type-1 matrix metalloproteinase (MT1-MMP) directly binds to gC1qR, a compartment-specific chaperone-like regulatory protein.
abstract: Membrane type-1 matrix metalloproteinase (MT1-MMP), a key enzyme in cell locomotion, is known to be primarily recruited to the leading edge of migrating cells. This raises a possibility that the C-terminal cytoplasmic tail of MT1-MMP interacts with intracellular regulatory proteins, which modulate translocations of the protease across the cell. Here, we demonstrated that MT1-MMP via its cytoplasmic tail directly associates with a chaperone-like compartment-specific regulator gC1qR. Although a direct functional link between these two proteins remains uncertain, our observations suggest that the transient associations of gC1qR with the cytoplasmic tail of MT1-MMP are likely to be involved in the mechanisms regulating presentation of the protease at the tumor cell surface.
authors.affiliation: The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Rozanov
authors.forename: Berhane
authors.initials: B
authors.lastname: Ghebrehiwet
authors.forename: BorisI
authors.initials: B
authors.lastname: Ratnikov
authors.forename: Edward Z
authors.initials: EZ
authors.lastname: Monosov
authors.forename: Elena I
authors.initials: EI
authors.lastname: Deryugina
authors.forename: Alex Y
authors.initials: AY
authors.lastname: Strongin
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D001943:Breast Neoplasms / Q000378:metabolism; D002352:Carrier Proteins; D002451:Cell Compartmentation; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors; D053504:Matrix Metalloproteinases, Membrane-Associated; D008562:Membrane Glycoproteins; D008666:Metalloendopeptidases / Q000378:metabolism*; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D011233:Precipitin Tests; D011951:Receptors, Complement / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: C087299:C1QBP protein, human; D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D010446:Peptide Fragments; D011951:Receptors, Complement; C032402:complement 1q receptor; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases