title: Highly efficient oligosaccharide synthesis on water-soluble polymeric primers by recombinant glycosyltransferases immobilised on solid supports.
abstract: Recombinant beta-1,4-galactosyltranferase (beta 1,4-GalT) and alpha-2,6-sialytransferase (alpha 2,6-SiaT) immobilised covalently with activated Sepharose beads were employed for the practical synthesis of a trisaccharide derivative, Neu-5Ac alpha(2-->6)Gal beta(1-->4)GlcNAc beta-O-(CH2)6-NH2, on a water-soluble primer having GlcNAc residues through a alpha-chymotrypsin-sensitive linker.
authors.affiliation: Sapporo Laboratory ofr Glycocluster Project, Japan Bioindustry Association, Sapporo 060-0810, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nishiguchi
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Fuji
authors.forename: S
authors.initials: S
authors.lastname: Shibatani
authors.forename: A
authors.initials: A
authors.lastname: Toda
authors.forename: S
authors.initials: S
authors.lastname: Nishimura
mesh_terms: D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism; D006001:Glycoconjugates / Q000138:chemical synthesis*; D006031:Glycosylation; D009237:N-Acetyllactosamine Synthase / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D011108:Polymers; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry; D012799:Sialyltransferases / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D014531:Uridine Diphosphate Galactose / Q000378:metabolism; D014867:Water; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D006001:Glycoconjugates; D009844:Oligosaccharides; D011108:Polymers; D011994:Recombinant Proteins; D014867:Water; D014531:Uridine Diphosphate Galactose; D012685:Sepharose; D009237:N-Acetyllactosamine Synthase; D012799:Sialyltransferases; D002918:Chymotrypsin; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase