[High-performance liquid chromatography determination of histamine in nasal mucosa of guinea pig after post-column derivatization].
A rapid and simple method for determining histamine by post-column derivatization liquid chromatography with fluorescent detection was described. SCX weakly acidic cation exchange column was used as an analytical column. Histamine was eluted at 13 min 12 s by 40 mmol.L-1 of trisodium citrate (pH5.50) at flow rate of 1.0 ml.min-1. The recoveries of histamine ranged from 1.0 mumol.L-1 to 100 mumol.L-1 were above 92%. The detection limit for histamine was 50 nmol.L-1 and the linear range for histamine was 50 nmol.L(-1)-500 mumol.L-1.
Huang
X H
XH
Department of Equipment Management, Hunan Medical University, Changsha 410078. Analytical and Testing Center, Hunan Medical University, Changsha 410078.
Chen
B M
BM
Liang
S X
SX
Deng
F L
FL
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006168:Guinea Pigs; D006632:Histamine / Q000032:analysis*; D066298:In Vitro Techniques; D009297:Nasal Mucosa / Q000737:chemistry*
D006632:Histamine