Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment.
Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene beta-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by beta-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which "model" cultivar was used.
Melchiorre
Mariana N
MN
Instituto de Fitopatología y Fisiología Vegetal, (IFFIVE-INTA), Camino 60 Cuadras Km 5o, (X5020ICA) Córdoba, Argentina. mmelchiorre@correo.inta.gov.ar
Lascano
Hernán R
HR
Trippi
Victorio S
VS
D000613:Aminobutyrates / Q000494:pharmacology*; D015139:Blotting, Southern; D018744:DNA, Plant; D004351:Drug Resistance; D005966:Glucuronidase / Q000378:metabolism; D006540:Herbicides / Q000494:pharmacology*; D008957:Models, Genetic; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D013997:Time Factors; D014908:Triticum / Q000235:genetics*
D000613:Aminobutyrates; D018744:DNA, Plant; D006540:Herbicides; C003121:phosphinothricin; D005966:Glucuronidase