title: The Purification and Renaturation of the Interleukin-2-Pseudomonas Exotoxin Fusion Protein (IL-2-PE).
abstract: The IL-2-PE fusion protein expressed in E. coli forms the inclusion body which can be isolated easily. The purity of the fusion protein was 90%-95% after washing the inclusion body with PBS containing 4 M urea and 0.5% Triton X-100 followed by chromatography on Sephacryl S-300 and DEAE-Sepharose FF. Then, parameters relative to the renaturation, including the concentrations of GSSG and L-Arg, the initiation concentrations of the protein, pH, temperature and Reaction time etc, were systematically analysed. An optimum condition for IL-2-PE renaturation was proposed.
authors.affiliation: The First Military Medical University, Guangzhou 510515, China.
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Gao
authors.forename: Zhong-Cheng
authors.initials: ZC
authors.lastname: Zheng
authors.forename: Xin-Mei
authors.initials: XM
authors.lastname: LinLai
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Wang