title: Reemergence, in southwestern Alaska, of invasive Haemophilus influenzae type b disease due to strains indistinguishable from those isolated from vaccinated children.
abstract: Haemophilus influenzae type b (Hib) invasive disease and oropharyngeal carriage continue in rural Alaska despite widespread vaccination. This study investigated whether invasive-disease reemergence during 1996-1997 could be attributed to strains distinguishable from strains carried by vaccinated children. Twenty-four invasive and 42 carriage Hib isolates, collected during 1992-1997, were characterized by pulsed-field gel electrophoresis (PFGE), multilocus enzyme electrophoresis, and biotyping. This Hib population was highly clonal, since only 2 strains, electrophoretic type (ET) 55/PFGE 1 and ET 56/PFGE 3, accounted for 62% of all isolates. The ET 55/PFGE 1 and ET 56/PFGE 3 strains were found in 74% of the carriers and caused 80% of the invasive Hib disease that occurred during April 1996-March 1997. Strains causing invasive disease could not be distinguished from strains carried by vaccinated children. Continued monitoring of Hib carriage may provide insights into the epidemiology of continued transmission in an era of widespread vaccination.
authors.affiliation: Arctic Investigations Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Anchorage, Alaska 99508, USA.
authors.forename: Lynne A
authors.initials: LA
authors.lastname: Lucher
authors.forename: Michael
authors.initials: M
authors.lastname: Reeves
authors.forename: Thomas
authors.initials: T
authors.lastname: Hennessy
authors.forename: Orin S
authors.initials: OS
authors.lastname: Levine
authors.forename: Tanja
authors.initials: T
authors.lastname: Popovic
authors.forename: Nancy
authors.initials: N
authors.lastname: Rosenstein
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Parkinson
mesh_terms: D000413:Alaska / Q000453:epidemiology; D002353:Carrier State / Q000453:epidemiology*; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006192:Haemophilus Infections / Q000453:epidemiology* / Q000517:prevention & control; D018073:Haemophilus Vaccines / Q000008:administration & dosage; D019741:Haemophilus influenzae type b / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D017720:Molecular Epidemiology; D014611:Vaccination
chemical_list: D018073:Haemophilus Vaccines