Development of an enzyme assay for rapid assessment of Escherichia coli in seawaters.
OBJECTIVE
An analytical protocol has been developed and applied for the detection of glucuronidase activity in marine waters as a rapid alternative approach to assess the microbiological quality of seawaters.


RESULTS
The fluorogenic substrate 4-methylumbelliferyl-beta-D-glucuronide is cleaved to a fluorescent product, methylumbelliferone, by the enzyme beta-glucuronidase, specific to Escherichia coli and closely related enterobacterial species (Shigella). The results suggest that this test is related to E. coli numbers, as estimated by immunofluorescence, more significantly than to faecal coliform numbers, obtained from culture media.


CONCLUSIONS
The determination of the potential rate of glucuronidase activity may be used as a diagnostic tool for the indirect estimation of the presence of E. coli in seawaters.


CONCLUSIONS
The method may be particularly useful in the early warning of seawater pollution, allowing the screening of coastal areas with different contamination levels in reduced time.
Caruso
G
G
Istituto Sperimentale Talassografico CNR, Messina, Italy. caruso@ist.me.cnr.it
Crisafi
E
E
Mancuso
M
M
D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification*; D005456:Fluorescent Dyes / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism*; D006923:Hymecromone / Q000031:analogs & derivatives* / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D013997:Time Factors; D014876:Water Pollution
D005456:Fluorescent Dyes; D006923:Hymecromone; C034888:4-methylumbelliferyl glucuronide; D005966:Glucuronidase