title: Apoptotic rate in peripheral T-cell lymphomas. A study using a tissue microarray with validation on full tissue sections.
abstract: Peripheral T-cell lymphomas (PTCLs) are a heterogeneous group of non-Hodgkin lymphomas with a wide spectrum of clinicopathologic features, and apoptosis mechanisms may have a role in lymphomagenesis. We assessed apoptotic rate (AR) in 112 PTCLs using a tissue microarray developed in our laboratory and a modified terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay. The mean AR was 1.47% +/- 1.38% for the entire group of PTCLs (range, 0.06%-5.15%), and AR varied significantly among different tumor types. In mycosis fungoides, the mean AR was 0.74%; angioimmunoblastic T-cell lymphoma, 1.02%; PTCL, not otherwise specified, 1.38%; cutaneous anaplastic large cell lymphoma (ALCL), 1.41%; anaplastic lymphoma kinase protein (ALK)-negative ALCL, 1.43%; extranodal natural killer/T-cell lymphoma of nasal type, 2.04%; ALK-positive ALCL, 2.95%; and enteropathy-type T-cell lymphoma, 3.06%. Mean AR was higher in PTCL with large cell vs small/medium cell morphologic features (1.66% +/- 1.1% vs 0.99% +/- 1.0%). In a subset of 33 PTCLs, the tissue microarray results comparedfavorably with those obtained in full tissue sections. We conclude that the highest ARs in PTCLs are found in enteropathy-type T-cell lymphoma and ALK-positive ALCL, and that AR can be assessed reliably by using a tissue microarray.
authors.affiliation: Department of Hematopathology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
authors.forename: George Z
authors.initials: GZ
authors.lastname: Rassidakis
authors.forename: Dan
authors.initials: D
authors.lastname: Jones
authors.forename: Athanasios
authors.initials: A
authors.lastname: Thomaides
authors.forename: Filiz
authors.initials: F
authors.lastname: Sen
authors.forename: Raymond
authors.initials: R
authors.lastname: Lai
authors.forename: Fernando
authors.initials: F
authors.lastname: Cabanillas
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: McDonnell
authors.forename: L Jeffrey
authors.initials: LJ
authors.lastname: Medeiros
mesh_terms: D000077548:Anaplastic Lymphoma Kinase; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000032:analysis; D002452:Cell Count; D002455:Cell Division; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007124:Immunoenzyme Techniques; D020287:In Situ Nick-End Labeling; D007694:Killer Cells, Natural / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000737:chemistry / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000737:chemistry / Q000473:pathology*; D011505:Protein-Tyrosine Kinases / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D020794:Receptor Protein-Tyrosine Kinases; D012878:Skin Neoplasms / Q000737:chemistry / Q000473:pathology; D016610:Tissue Embedding / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D019253:Proto-Oncogene Proteins c-bcl-2; C000626173:ALK protein, human; D000077548:Anaplastic Lymphoma Kinase; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases