Inhibition of Glutamate Uptake by Superoxide Anion Radical in Rat Cortical Synaptosomes and the Protective Effect of Ebselen.
The inhibition of glutamate uptake by superoxide anion radical (O(-)(2).) in rat cortical synaptosomes and the protective effect of Ebselen was studied by radioisotope method. The exposure to xanthine/xanthine oxidase, a O(-)(2).-generating system, resulted in a marked decrease of high-affinity glutamate transport in the synaptosomes. In parallel, the Na(+),K(+)-ATPase activity was also damaged, while the lactate dehydrogenase activity and the TBARS contents in synaptic culture were not affected. It suggests that the inhibition of glutamate uptake is related to the damage of Na(+), K(+)-ATPase by O(-)(2). Ebselen was showed to have a blocking effect on the glutamate uptake inhibition by O(-)(2)., probably through protecting the Na(+),K(+)-ATPase.
Yi
Yong
Y
Department of Chemistry, Huazhong University of Science Technology, Wuhan 430074, China.
Yang
Xiang-Liang
XL
Xu
Hui-Bi
HB
Zhao
Xi-Long
XL
Zhang
Heng-Shan
HS
Qin
Yu-Hui
YH