Effects of chlorpromazine, pentoxifylline and dexamethasone on mRNA expression of lipopolysaccharide-induced inflammatory cytokines in bovine peripheral blood mononuclear cells.
The effects of chlorpromazine (CPZ), pentoxifylline (PTX) and dexamethasone (DEX) on mRNA expression of lipopolysaccharide (LPS)-induced proinflammatory cytokines were examined in bovine peripheral blood mononuclear cells (PBMCs) in vitro. The expression of inflammatory cytokine mRNAs was analyzed by RT-PCR and Southern blot hybridization in bovine PBMCs. CPZ and DEX decreased the expression of cytokine mRNA (such as interleukin-1 beta and tumor necrosis factor-alpha) after stimulation with LPS in a dose-dependent manner. However, pretreatment with PTX had no inhibitory effect on the mRNA expression of proinflammatory cytokines. These results indicated that pretreatment with CPZ and DEX might be effective to reduce the production of LPS-induced inflammatory cytokines in bovine PBMCs in vitro.
Kiku
Yoshio
Y
Department of Veterinary Pathology, large animal Clincal Center, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Matsuzawa
Hironori
H
Ohtsuka
Hiromichi
H
Terasaki
Nobuhiro
N
Fukuda
Shigeo
S
Kon-Nai
Satoru
S
Koiwa
Masateru
M
Yokomizo
Yuichi
Y
Sato
Hiroshi
H
Rosol
Thomas J
TJ
Okada
Hiroyuki
H
Yoshino
Tomo-o
TO
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D015139:Blotting, Southern; D002417:Cattle; D002746:Chlorpromazine / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D005786:Gene Expression Regulation; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D010431:Pentoxifylline / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D003907:Dexamethasone; D010431:Pentoxifylline; D002746:Chlorpromazine