Physical properties of detergent-based aqueous two-phase systems.
The physical behavior of the binary phase systems of the non-ionic polyoxyethylene detergent Agrimul NRE 1205 and water was investigated. This technical detergent can be used for the large-scale recovery of biomolecules in detergent based aqueous two-phase systems. The phase diagram was determined. It shows significant and unexpected differences to highly purified detergents. Very similar to neat detergents the phase diagram can be influenced by auxiliary chemicals thus shifting the entire phase diagram in general to lower temperatures. This was demonstrated by lowering the cloud-point by various additions. The concentration factor, as an important parameter of a first capture step in purification was investigated and modeled. Auxiliary chemicals, temperature change and change in detergent concentration also influence the viscosity and density of the phases. These experimental data are shown. They can help to explain the separation behavior of proteins. In large-scale separations aqueous two-phase systems are separated using disc-stack centrifuges. It is demonstrated that this is not a feasible method for detergent-based aqueous two-phase extraction and the physical reason is presented.
Selber
K
K
Institut für Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, Jülich, Germany.
Müller
S
S
Gieren
H
H
Thömmes
J
J
Sottmann
T
T
Strey
R
R
Kula
M R
MR
D001709:Biotechnology / Q000379:methods; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003902:Detergents; D011092:Polyethylene Glycols; D011506:Proteins / Q000302:isolation & purification*; D014783:Viscosity; D014867:Water
D003902:Detergents; D011506:Proteins; D014867:Water; D011092:Polyethylene Glycols