[A study on hemostatic and immunological actions of fresh and dry Dihuang].
OBJECTIVE
To investigate the difference between fresh and dry Dihuang (Radix Rehmanniae) in hemostatic and immunological actions.


METHODS
The hemostatic action was assessed in aspirin-treated mice; immunological action was assessed by phagocytosis of macrophages in dexamethasone-treated mice; and Con A-induced mitogenesis and LPS-induced alkaline phosphatase activity of splenocytes were determined in thyroxin-treated mice.


RESULTS
The blood clotting in mice induced by aspirin was inhibited by oral administrations of juice decoction of fresh Dihuang or decoction of dry Dihuang alike. The action of fresh Dihuang juice was stronger than that of dry Dihuang decoction. Oral administrarion of fresh Dihuang juice or decoction for 10 days enhanced LPS-induced alkaline phosphatase activity of splenocytes in thyroxin-treated mice. The juice also enhanced Con A-induced splenocyte mitogenesis. The action of dry Dihuang decoction was weaker than that of fresh juice in increasing LPS-induced alkaline phosphatase activity.


CONCLUSIONS
It is recommended to follow the tradition of using fresh Dihuang juice or decoction to treat some diseases such as hemorrhagenic troubles.
Liang
A
A
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700.
Xue
B
B
Wang
J
J
Hao
J
J
Yang
H
H
Yi
H
H
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006490:Hemostatics / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000187:drug effects; D018517:Plant Roots; D010946:Plants, Medicinal; D011897:Random Allocation; D032267:Rehmannia
D004365:Drugs, Chinese Herbal; D006490:Hemostatics