Investigation of the possibility of geometrical electrophoresis.
We investigate the possibility of geometrical electrophoresis, which is based on nanofabrication techniques. (GEE) utilizes geometrical effects during electrophoresis, which are generated by physical interactions between walls and a macromolecule confined in spaces smaller than the Flory radius. When a polymer is injected into a small space, confinement energy is usually required. However, the confinement energy form depends on the geometry of the space. In the case of electrophoresis, the electric field itself changes depending on the geometry. Using a nanofabricated quartz chip with a curved channel, we investigated electrophoretic behavior of high molecular weight DNA based on the curvature effect.
Ueda
Masanori
M
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78 Shohmachi, Tokushima 770-8505, Japan. uedam@v006.vaio.ne.jp
Hayama
Tetsuya
T
Takamura
Yuzuru
Y
Horiike
Yasuhiro
Y
Baba
Yoshinobu
Y
D004247:DNA / Q000302:isolation & purification*; D011088:DNA Ligases / Q000302:isolation & purification; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D008904:Miniaturization; D036103:Nanotechnology; D009690:Nucleic Acid Conformation; D011791:Quartz
D011791:Quartz; D004247:DNA; D011088:DNA Ligases