title: Remineralization of advanced root dentin lesions in vitro.
abstract: The possibility for remineralization of advanced root dentin lesions was investigated in vitro. Lesions were produced in acidic buffer solution with 0.1 and 0.5 ppm fluoride, for 3 and 5 weeks, respectively, using a single-section model. Next, the sections were immersed in a remineralization solution containing 0.1 ppm F (as NaF) for 20 weeks. Microradiographs were made after each 4-week-period. The lesions were around 500 micro m deep, with mineral loss values between 11,000 and 18000 vol%.microm. After 20 weeks of remineralization, the absolute amounts of deposited mineral ranged from 7500 to 10000 vol%. micro m, corresponding to a repair between 50 and 85%. Lesion depth was, on average, reduced by 199 micro m. This was not significantly different (at p < 0.05) between the four experimental groups. This study showed that remineralization of advanced dentinal lesions is possible in spite of the virtual absence of mineral in the lesion body after demineralization. The surface layer of the lesion was the preferred site for mineral deposition until mineral content values reached those of sound dentine. The mineral content in the lesion body increased with time, at a rate reflecting the initial severity of the lesion. We conclude that a remineralized surface layer does not inhibit transport of mineral ions to the body and that the remineralization in the lesion body reflected the number of sites for crystal growth.
authors.affiliation: Department of Cariology, Endodontology, Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands. mukaiyos@kdcnet.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Mukai
authors.forename: J M
authors.initials: JM
authors.lastname: ten Cate
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002122:Calcium Chloride / Q000627:therapeutic use; D002327:Cariostatic Agents / Q000493:pharmacokinetics / Q000627:therapeutic use*; D002417:Cattle; D003804:Dentin / Q000378:metabolism; D066298:In Vitro Techniques; D008852:Microradiography; D010710:Phosphates / Q000627:therapeutic use; D017680:Potassium Compounds / Q000627:therapeutic use; D017213:Root Caries / Q000188:drug therapy*; D012969:Sodium Fluoride / Q000493:pharmacokinetics / Q000627:therapeutic use*; D017001:Tooth Demineralization / Q000188:drug therapy*; D014089:Tooth Remineralization / Q000379:methods*; D014092:Tooth Root / Q000378:metabolism
chemical_list: D002327:Cariostatic Agents; D010710:Phosphates; D017680:Potassium Compounds; D012969:Sodium Fluoride; C013216:potassium phosphate; D002122:Calcium Chloride