Analysis of involvement of the 3'-untranslated regions in regulating mRNA stability for vitellogenin, cyanoprotein alpha, and cyanoprotein beta from the bean bug, Riptortus clavatus.
The degradation of the 3'-untranslated regions (UTRs) of vitellogenin, cyanoprotein alpha, and cyanoprotein beta from the bean bug, Riptortus clavatus, was analyzed in vitro. The degradation pattern was similar for all three RNAs, with a high degradation rate in non-diapausing adult insects and no degradation in the fifth instar nymphs and in diapausing adults, and was not correlated with the expression levels of these three proteins. Proteins binding to the 3'-UTRs were detected in polysomal and cytosolic extracts. These factors, however, were present in all developmental stages. The abundance of the polysomal factor showed little variation, but the cytosolic factor was enriched in adult insects. Cross-competition experiments demonstrated that the same factors bound to all three RNAs with similar affinity. The pattern of degradation, presence of the binding factors in all stages, and their inability to distinguish between the target sequences indicate that the 3'-UTRs do not participate in controlling the expression of these three proteins.
Wojtasek
Hubert
H
Department of Medical Zoology, Mie University, School of Medicine, Tsu, Japan. wojtasek@uni.opole.pl
Miura
Ken
K
Shinoda
Tetsuro
T
Chinzei
Yasuo
Y
D020413:3' Untranslated Regions / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation; D006430:Hemiptera / Q000235:genetics*; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D014819:Vitellogenins / Q000737:chemistry / Q000235:genetics*
D020413:3' Untranslated Regions; D019476:Insect Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014819:Vitellogenins; C041137:storage proteins, Insecta