[Modulation of tonic herbs on the immune cell inhibition in stressed model animal and its possible mechanism].
OBJECTIVE
To observe the effect of tonic herbs on the immune cell proliferation in stressed mice.


METHODS
A model of stressed mice was built to observe the effect of such tonic herbs as Tusizi, Chuanduan; Nüzhenzi, Gouqizi on the calcium concentration in cytoplasm in the process of immune cell reproduction, as well as on the membrane fluidity of splenetic lymphocytes, IL-2, IL-2R and cell period.


RESULTS
The tonic herbs could improve the proliferation ability of spleen lymphocyte in the stressed mice, reduce the calcium concentration, and recover the fluidity of cell membrane. They could also improve the activity of IL-2 and increase the amount of IL-2R cell(P < 0.01). The combination of the four tonic herbs proved more effective, facilitating the cell into DNA synthetic period and reducing the retention period in G0/G1 (P < 0.01).


CONCLUSIONS
Tonic herbs can modulate the reproductive function of the spleen lymphocyte and relieve the unfavorable response of the stress on the organism.
Qian
R Q
RQ
Department of Integration of Chinese and Western Medicine, Beijing Medical University, Beijing 100083, China.
Meng
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Y
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000097:blood; D002455:Cell Division / Q000187:drug effects; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007376:Interleukin-2 / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010946:Plants, Medicinal; D011897:Random Allocation; D013312:Stress, Physiological / Q000276:immunology*
D000276:Adjuvants, Immunologic; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007376:Interleukin-2; D002118:Calcium