title: Alpha-glucosidase inhibitor from the seeds of balsam pear (Momordica charantia) and the fruit bodies of Grifola frondosa.
abstract: Alpha-glucosidase inhibitory activities were found in aqueous methanol extracts of the seeds of Momordica charantia and the fruit bodies of Grifola frondosa. An active principle against the enzyme prepared from rat small intestine acetone powders was isolated and characterized. The structure of the isolated compound was identified as D-(+)-trehalose by FDMS, 1H-, 13C-NMR, and [alpha]D measurements. The inhibitory activity of trehalose was compared with 1-deoxynojirimycin. Trehalose showed 45% inhibitory activity at the concentration of 2 x 10(-3) m, but 1-deoxynojirimycin had 52% inhibitory activity at 1 x 10(-7) M.
authors.affiliation: Northern Advancement Center for Science & Technology, Sapporo, Japan. matsuura@chem.agr.hokudai.ac.jp
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsuur
authors.forename: Chikako
authors.initials: C
authors.lastname: Asakawa
authors.forename: Masanori
authors.initials: M
authors.lastname: Kurimoto
authors.forename: Junya
authors.initials: J
authors.lastname: Mizutani
mesh_terms: D017485:1-Deoxynojirimycin / Q000494:pharmacology; D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D002240:Carbohydrate Sequence; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D029775:Momordica / Q000737:chemistry*; D051381:Rats; D012639:Seeds / Q000737:chemistry; D014199:Trehalose / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D017485:1-Deoxynojirimycin; D014199:Trehalose