Inhibition of inducible nitric oxide synthesis by catalposide from Catalpa ovata.
Catalposide (1) and two related iridoids were isolated from the stem of Catalpa ovata (Bignoniaceae) by bioassay guided fractionation. Catalposide (1) significantly inhibited the production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages in a dose-dependent manner. RT-PCR and Western blot analyses demonstrated that catalposide (1) suppressed the expression of inducible nitric oxide synthase (iNOS) gene and iNOS protein. Catalposide (1) also inhibited the activation of LPS-induced NF-kappaB as analyzed by electrophoretic mobility shift assay (EMSA). In addition to the inhibitory effect on NO production in LPS-stimulated RAW 264.7 cells, catalposide (1) significantly inhibited the NO production in cytokine-stimulated human DLD-1 and rat vascular smooth muscle (VSM) cells in a dose-dependent manner.
Oh
Hyuncheol
H
Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
Pae
Hyun-Ock
HO
Oh
Gi-Su
GS
Lee
Seung Yeob
SY
Chai
Kyu-Yun
KY
Song
Choong Eui
CE
Kwon
Tae-Oh
TO
Chung
Hun-Taeg
HT
Lee
Ho-Sub
HS
D000818:Animals; D029402:Bignoniaceae / Q000737:chemistry*; D002460:Cell Line; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005960:Glucosides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D015394:Molecular Structure; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D005960:Glucosides; D012333:RNA, Messenger; C006928:catalposide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse