[Rapid induction of mRNAs for liver regeneration genes by hepatopoietin and partial hepatectomy].
OBJECTIVE
To investigate the effect of recombinant human hepatopoietin (rhHPO) and partial hepatectomy on rapidly induced expression of immediate early gene.


METHODS
We investigated the different gene expression within 1 hour after 2/3 partial hepatectomy by representational difference analysis and in primary cultured hepatocytes system.


RESULTS
In the expressed sequence tag (EST) library, we identified that most of these genes were immediate early gene, and found one new gene PC3 that might be associated to liver regeneration in the EST library. Moreover, PC3 gene was rapidly induced after 2/3 partial hepatectomy and the expressing peak was within 1~2 hours after operation. HPO can rapidly induce the expression of these genes (c-fos, LRF-1, and PC3, etc.) in primarily cultured rat hepatocyte, which might be one of HPO molecular mechanism on stimulating hepatocyte proliferation.


CONCLUSIONS
rhHPO and partial hepatectomy can rapidly induce the expression of immediate early gene. PC3 gene is immediate early gene related to liver regeneration.
Wang
Ge
G
Department of Digestive Diseases, Daping Hospital of Third Military Medical University, Chongqing 400042, China.
Zhang
Xiao Rong
XR
Hu
Lu
L
Wang
Jun
J
Leng
En Ren
ER
Fang
Dian Chun
DC
Yang
Xiao Ming
XM
Zhang
Yong
Y
He
Fu Chu
FC
D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D015152:Blotting, Northern; D005786:Gene Expression Regulation / Q000187:drug effects; D017781:Genes, Immediate-Early; D006498:Hepatectomy; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D008115:Liver Regeneration / Q000235:genetics*; D043484:Proprotein Convertases; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology
D012333:RNA, Messenger; D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor; D043484:Proprotein Convertases; D016282:Aspartic Acid Endopeptidases