title: IL-15 up-regulates iNOS expression and NO production by gingival epithelial cells.
abstract: To investigate the biological activity of epithelial cells in view of host defense, we analyzed the mRNA expression of inducible NOS (iNOS) as well as NO production by human gingival epithelial cells (HGEC) stimulated with IL-15. RT-PCR analysis revealed that HGEC expressed IL-15 receptor alpha-chain mRNA. In addition, stimulation with IL-15 enhanced iNOS expression by HGEC through an increase of both mRNA and protein levels. Moreover, IL-15 up-regulated the production of NO(2)(-)/NO(3)(-), a NO-derived stable end product, from HGEC. The enhanced NO production by IL-15 was inhibited by AMT, an iNOS-specific inhibitor. These results suggest that IL-15 is a potent regulator of iNOS expression by HGEC and involved in innate immunity in the mucosal epithelium.
authors.affiliation: Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Yanagita
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Shimabukuro
authors.forename: Takenori
authors.initials: T
authors.lastname: Nozaki
authors.forename: Naoko
authors.initials: N
authors.lastname: Yoshimura
authors.forename: Junko
authors.initials: J
authors.lastname: Watanabe
authors.forename: Hiroko
authors.initials: H
authors.lastname: Koide
authors.forename: Mami
authors.initials: M
authors.lastname: Terakura
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Saho
authors.forename: Masahide
authors.initials: M
authors.lastname: Takedachi
authors.forename: Myoung-Ho
authors.initials: MH
authors.lastname: Jang
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyono
authors.forename: Shinya
authors.initials: S
authors.lastname: Murakami
mesh_terms: D002469:Cell Separation; D002478:Cells, Cultured; D015638:Cytochalasin D / Q000494:pharmacology; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005434:Flow Cytometry; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D019409:Interleukin-15 / Q000235:genetics / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D004791:Enzyme Inhibitors; D019409:Interleukin-15; D019384:Nucleic Acid Synthesis Inhibitors; D012333:RNA, Messenger; D015638:Cytochalasin D; D009569:Nitric Oxide; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II