title: Establishment and characterization of a lymphoepithelial-like carcinoma cell line (HUUCLEC) derived from the human uterine cervix.
abstract: A cell line designated HUUCLEC was established from a human uterine cervical lymphoepithelial carcinoma obtained from a 61-year-old Japanese woman. The cell line has grown slowly without interruption and serial passages were successively carried out 60 times within 3 years. The cultured cells were spindle or round in shape, showing anaplastic and pleomorphic features, a pavement cell arrangement and multilayering without contact inhibition. The population doubling time of the HUUCLEC line was 72 hours while the chromosomal number varied widely and showed aneuploidy. The modal chromosomal number was stable at the triploid range and marker chromosomes were present; the Ebstein-Barr virus was absent in the cultured cells.
authors.affiliation: Dept Ob & Gy, Tokyo Hosp, Sch of Med, St Marianna Univ, Japan.
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kiguchi
authors.forename: Isamu
authors.initials: I
authors.lastname: Ishiwata
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tokieda
authors.forename: Megumi
authors.initials: M
authors.lastname: Iguchi
authors.forename: Chieko
authors.initials: C
authors.lastname: Ishiwata
authors.forename: Masanori
authors.initials: M
authors.lastname: Iwata
authors.forename: Bunpei
authors.initials: B
authors.lastname: Ishizuka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Tachibana
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikawa
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology* / Q000821:virology; D006224:Cricetinae; D005260:Female; D006801:Humans; D007621:Karyotyping; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009368:Neoplasm Transplantation; D027383:Papillomaviridae / Q000302:isolation & purification; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology* / Q000821:virology