title: Use of a highly sensitive quantitative telomerase assay in intracytoplasmic sperm injection programmes for the treatment of 47,XXY non-mosaic Klinefelter men.
abstract: We evaluated the role of the sensitive quantitative telomerase assay (SQTA) in the management of men with non-mosaic Klinefelter's syndrome (KS). Diagnostic testicular biopsy (DTB) was performed in 24 men with KS. A part of the DTB was stained and the remaining fragment was processed for the SQTA. After 3-18 months, a therapeutic testicular biopsy (TTB) was performed in the same testicle and the recovered specimens were processed to identify spermatozoa. Men with a SQTA outcome equal to 0.00 Units microg-1 protein (n = 7) demonstrated therapeutic testicular biopsy material that was negative for spermatogenic cells. In five men with a SQTA outcome of 8.11-38.03 Units microg-1, the most advanced germ cell was the spermatogonium/primary spermatocyte. In the remaining 12 men, the most advanced spermatogenic cell in the TTB was the spermatozoon. In these men, the SQTA outcome was equal to 25.76-92.68 Units microg-1 protein. Using 39.00 Units microg-1 protein as a cut-off value, the accuracy of the SQTA in identifying men positive for spermatozoa was 91.6%. It appears that the SQTA has a role for identifying non-mosaic KS men who have testicular spermatozoa.
authors.affiliation: Tottori University School of Medicine, Tottori, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Sofikitis
authors.forename: A
authors.initials: A
authors.lastname: Kaponis
authors.forename: J
authors.initials: J
authors.lastname: Georgiou
authors.forename: D
authors.initials: D
authors.lastname: Giannakis
authors.forename: Ch
authors.initials: Ch
authors.lastname: Mamoulakis
authors.forename: D
authors.initials: D
authors.lastname: Loutradis
authors.forename: X
authors.initials: X
authors.lastname: Yiannakopoulos
authors.forename: Y
authors.initials: Y
authors.lastname: Mio
authors.forename: I
authors.initials: I
authors.lastname: Miyagawa
authors.forename: A
authors.initials: A
authors.lastname: Chatzikyziakidou
mesh_terms: D000328:Adult; D001706:Biopsy; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007713:Klinefelter Syndrome / Q000235:genetics* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009030:Mosaicism; D012680:Sensitivity and Specificity; D020554:Sperm Injections, Intracytoplasmic; D019098:Telomerase / Q000378:metabolism*; D013737:Testis / Q000473:pathology
chemical_list: D019098:Telomerase