title: Anti-phospholipid antibodies in diabetes mellitus.
abstract: The presence of autoantibodies to phospholipids may be associated with various pathological disorders; diabetes could be one of them because of the changes occurring in lipid metabolism but there are only few reports examining this question, and they are not always leading to the same conclusions because of the differences in the procedures or in the phospholipids tested. We carried out a systematic comparative study of diabetic serum antibody binding to all phospholipids, anionic and zwitterionic, by a quantitative ELISA. The implication of the hydrophobic moiety of the lipids was also studied: the presence of autoantibodies to the fatty acyl chains was investigated. Our results show the presence of anti-phospholipid antibodies in diabetic sera, particularly anti-phosphatidylinositol and anti-phosphatidylcholine which have never been tested before, and appear to be associated with macroangiopathic complications. The antigenic epitopes are mainly the polar heads as no antibody binding to the hydrophobic moiety was observed. We discuss the relation of those antibodies to the angiopathic complications and to the direct effects of hyperglycemia on lipid antigenicity.
authors.affiliation: Laboratoire de Biogenèse Membranaire, CNRS-UMR 5544, Université Victor Segalen Bordeaux, France.
authors.forename: Henri
authors.initials: H
authors.lastname: Gin
authors.forename: Vincent
authors.initials: V
authors.lastname: Vergnot
authors.forename: Vicky
authors.initials: V
authors.lastname: Diakou
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Perret
authors.forename: Jerĵme
authors.initials: J
authors.lastname: Poix
authors.forename: Claude
authors.initials: C
authors.lastname: Cassagne
authors.forename: Lilly
authors.initials: L
authors.lastname: Maneta-Peyret
mesh_terms: D000328:Adult; D017152:Antibodies, Antiphospholipid / Q000097:blood*; D001786:Blood Glucose / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000276:immunology*; D003925:Diabetic Angiopathies / Q000097:blood / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000276:immunology; D010716:Phosphatidylinositols / Q000276:immunology; D012016:Reference Values
chemical_list: D017152:Antibodies, Antiphospholipid; D001786:Blood Glucose; D006442:Glycated Hemoglobin; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols