title: Monitoring cytomegalovirus infection by antigenemia assay and two distinct plasma real-time PCR methods after hematopoietic stem cell transplantation.
abstract: We compared a CMV virus load determined by real-time PCR with an antigenemia value to analyze the correlation between these two methods. We also compared the values for virus load determined by the two distinct real-time PCR methods, which amplify the US17 region and immediate-early (IE) gene of CMV, respectively, to evaluate the reliability of these methods. Two hundred and sixty-five samples were obtained weekly from 29 patients, who had engraftment after unrelated bone marrow transplantation or HLA-mismatched related blood stem cell transplantation. CMV infection was detected in 115 samples from 22 patients by US17-PCR and 69 samples from 20 patients by the antigenemia assay. Fifty-eight samples were positive for both assays, but 57 and 11 samples were positive only for US17-PCR and antigenemia, respectively. A good correlation of the results of US17-PCR and antigenemia was demonstrated (r = 0.61). All antigenemia-positive samples and randomly selected antigenemia-negative samples were subjected to IE-PCR. The results of IE-PCR showed a good correlation with those of antigenemia (r = 0.64). Furthermore, the best correlation was observed between US17-PCR and IE-PCR (r = 0.83). In conclusion, both real-time PCR methods showed a good correlation with the antigenemia assay, and could be used to monitor CMV infection after hematopoietic stem cell transplantation.
authors.affiliation: Department of Hematology, Tokyo Metropolitan Komagome Hospital, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Kanda
authors.forename: M
authors.initials: M
authors.lastname: Kami
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Hamaki
authors.forename: E
authors.initials: E
authors.lastname: Kusumi
authors.forename: S
authors.initials: S
authors.lastname: Miyakoshi
authors.forename: Y
authors.initials: Y
authors.lastname: Nannya
authors.forename: S
authors.initials: S
authors.lastname: Chiba
authors.forename: Y
authors.initials: Y
authors.lastname: Arai
authors.forename: K
authors.initials: K
authors.lastname: Mitani
authors.forename: H
authors.initials: H
authors.lastname: Hirai
authors.forename: Y
authors.initials: Y
authors.lastname: Mutou
mesh_terms: D000956:Antigens, Viral / Q000097:blood*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000097:blood*; D017781:Genes, Immediate-Early / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D017874:Immediate-Early Proteins / Q000097:blood / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D012698:Serologic Tests; D019562:Viral Load / Q000379:methods
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D017874:Immediate-Early Proteins