title: Egg yolk platelet proteins from Xenopus laevis are amyloidogenic.
abstract: The association of amphibian (Xenopus laevis) egg yolk platelet proteins, represented predominantly by lipovitellin, was studied as a model of the formation of amyloid deposits. Two kinds of molecular organization formed by this protein material - native and heat-denatured - were found to exhibit amyloid properties although they differ significantly in structural organization. The first consisted in protein molecules arranged in the natural, physiological, net-like platelet organization, with a tendency to orient uni-directionally. The second was obtained by the gradual removal of Congo red from lipovitellin denatured by heating in an excess of dye. This procedure produced the twisted fibrillar organization of molecules typical for amyloids, represented predominantly by end-to-end associated major polypeptide chains of lipovitellin. Both native and denatured structural forms bind Congo red and produce a green birefringence effect, confirming the near parallel alignment of the complexed Congo red molecules. However, a dye(1,4-bis(1-amino-4-sulfonaphtyl-2-azo)phenylene) closely related to Congo red but with a very weak self-assembling tendency appeared inactive when the spectral shift was studied in a cross-polarization system, indicating in this way that dye supramolecularity is an extra factor which may determine binding to amyloid proteins and specific spectral effects.
authors.affiliation: Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University,Cracow, Poland.
authors.forename: G
authors.initials: G
authors.lastname: Zemanek
authors.forename: L
authors.initials: L
authors.lastname: Konieczny
authors.forename: B
authors.initials: B
authors.lastname: Piekarska
authors.forename: J
authors.initials: J
authors.lastname: Rybarska
authors.forename: B
authors.initials: B
authors.lastname: Stopa
authors.forename: P
authors.initials: P
authors.lastname: Spólnik
authors.forename: B
authors.initials: B
authors.lastname: Urbanowicz
authors.forename: M
authors.initials: M
authors.lastname: Nowak
authors.forename: M
authors.initials: M
authors.lastname: Król
authors.forename: I
authors.initials: I
authors.lastname: Roterman
mesh_terms: D000682:Amyloid / Q000737:chemistry; D000686:Amyloidosis; D000818:Animals; D001792:Blood Platelets / Q000502:physiology; D004527:Egg Proteins / Q000378:metabolism*; D005260:Female; D010063:Ovum / Q000166:cytology* / Q000502:physiology; D011489:Protein Denaturation; D014982:Xenopus laevis
chemical_list: D000682:Amyloid; D004527:Egg Proteins