Migration of bovine spermatozoa in a synthetic medium and its relation to in vivo bull fertility.
Although sperm migration has been extensively refined and validated in human infertility studies, its application to predict bovine fertility has been very limited, and a clear relation between the sperm migration distance and in vivo bull fertility has never been demonstrated. A synthetic medium based upon methyl cellulose (MC) was tested for its suitability to serve as a migration medium for frozen-thawed bovine spermatozoa. The effects of the concentration of MC, the incubation time, and sperm concentration on sperm migration capacity was determined. The relation between sperm migration capacity at different incubation times of the frozen-thawed spermatozoa of five bulls, and their 56 days nonreturn rates (NRRs) was assessed in order to evaluate its suitability as a tool to predict in vivo bull fertility. The highest repeatability of the sperm migration test (CV = 10.7%) was obtained when the sperm migration distance of the five vanguard motile spermatozoa was determined at 30 min incubation at 37 degrees C in a migration medium with 1.35% MC. No significant difference in migration distance was demonstrated when sperm concentrations of 100 x 10(6) and 150 x 10(6) spermatozoa/ml, respectively, were used. Despite the relatively high repeatability of the migration test, no relation was found between the sperm migration distance and the 56 days NRRs of five sire bulls. Therefore, the sperm migration test in 1.35% MC cannot be used to predict in vivo bull fertility accurately.
Verberckmoes
S
S
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. stevenv.verberckmoes@rug.ac.be
Van Soom
A
A
De Pauw
I
I
Dewulf
J
J
de Kruif
A
A
D000818:Animals; D002417:Cattle; D015925:Cryopreservation; D005298:Fertility; D006863:Hydrogen-Ion Concentration; D008297:Male; D008747:Methylcellulose; D015203:Reproducibility of Results; D012662:Semen Preservation / Q000662:veterinary; D012996:Solutions; D013076:Sperm Count; D013081:Sperm Motility
D012996:Solutions; D008747:Methylcellulose