Potential regulatory function of human dendritic cells expressing indoleamine 2,3-dioxygenase.
Antigen-presenting cells (APCs) can induce tolerance or immunity. We describe a subset of human APCs that express indoleamine 2,3-dioxygenase (IDO) and inhibit T cell proliferation in vitro. IDO-positive APCs constituted a discrete subset identified by coexpression of the cell-surface markers CD123 and CCR6. In the dendritic cell (DC) lineage, IDO-mediated suppressor activity was present in fully mature as well as immature CD123+ DCs. IDO+ DCs could also be readily detected in vivo, which suggests that these cells may represent a regulatory subset of APCs in humans.
Munn
David H
DH
Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA. dmunn@mail.mcg.edu
Sharma
Madhav D
MD
Lee
Jeffrey R
JR
Jhaver
Kanchan G
KG
Johnson
Theodore S
TS
Keskin
Derin B
DB
Marshall
Brendan
B
Chandler
Phillip
P
Antonia
Scott J
SJ
Burgess
Russell
R
Slingluff
Craig L
CL
Mellor
Andrew L
AL
D000938:Antigen-Presenting Cells / Q000201:enzymology / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis; D002448:Cell Adhesion; D019070:Cell Lineage; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000201:enzymology* / Q000276:immunology*; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007371:Interferon-gamma / Q000494:pharmacology; D016753:Interleukin-10 / Q000494:pharmacology; D053650:Interleukin-3 Receptor alpha Subunit; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008221:Lymphoid Tissue / Q000166:cytology / Q000201:enzymology; D008264:Macrophages / Q000201:enzymology; D054399:Receptors, CCR6; D019707:Receptors, Chemokine / Q000032:analysis; D016185:Receptors, Interleukin-3 / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000494:pharmacology; D014366:Tryptophan Oxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D015703:Antigens, CD; C106311:CCR6 protein, human; D004791:Enzyme Inhibitors; C115022:IL3RA protein, human; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D053650:Interleukin-3 Receptor alpha Subunit; D054399:Receptors, CCR6; D019707:Receptors, Chemokine; D016185:Receptors, Interleukin-3; D016753:Interleukin-10; D007371:Interferon-gamma; D014364:Tryptophan; D014366:Tryptophan Oxygenase