title: Preparation of Neoglycoproteins by Using Homo-bifunctional Reagent as the Coupling Agent.
abstract: Several neoglycoproteins (Man-BSA, Gal-BSA, Glc-BSA, Lac-BSA, GlcNAc-BSA, Fuc-BSA and Rha-BSA) have been prepared by using homo-bifunctional reagents (divinyl sulfone, DVS and 1,4-butanediol diglycidyl ether, Epoxy) as coupling agents and purilied with the respective immobilized lectins (except Fuc-BSA and Rha-BSA). These purified neoglycoproteins showed binding specificity to the corresponding lectins by hemagglutinating inhibition test and double diffusion experiment.
authors.affiliation: Cancer Hospital, Shanghai Medical University, Shanghai 200032, China.
authors.forename: Da-Ren
authors.initials: DR
authors.lastname: Shi
authors.forename: Xiang
authors.initials: X
authors.lastname: Du
authors.forename: Ce
authors.initials: C
authors.lastname: Sun
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang