title: Immunohistochemical localization and mRNA expression of apolipoprotein A-I in rat spinal cord.
abstract: Apolipoproteins in the cerebrospinal fluid (CSF) play important roles in lipid metabolism in the central nervous system. Although it has been demonstrated that apo E is synthesized in the neuron, the synthesis of apo A-I has only been determined in fish and chicken. It was demonstrated that apo A-I concentrations in the CSF were increased in poliovirus-infected macaques, however, the origin of the CSF apo A-I was not determined. The present immunohistochemical study provided evidence that apo A-I was localized within the nerve cell body of the rat spinal cord. In situ hybridization also showed that apo A-I mRNA was predominantly expressed in the neurons. As a further experiment, we compared apo A-I levels in the spinal cord from control rats and rats with experimental allergic encephalomyelitis (EAE), which was induced by sensitization with myelin basic protein. Although no significant changes in serum apo A-I levels were observed, apo A-I levels in the spinal cord were significantly elevated in EAE rats. Furthermore, apo A-I in the spinal cord of rats with EAE was not seen in the nerve cell body, but at the interstitium, particularly in lesions where inflammation had occurred. The current study clearly demonstrated that apo A-I is synthesized in the neurons of the rat spinal cord and the synthesis was suppressed in EAE rats.
authors.affiliation: Department of Laboratory Medicine, Gifu University School of Medicine, Japan.
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Fujii
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Saito
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Hamakawa
authors.forename: Naoya
authors.initials: N
authors.lastname: Maekawa
authors.forename: Suwako
authors.initials: S
authors.lastname: Fujigaki
authors.forename: Hisayasu
authors.initials: H
authors.lastname: Wada
authors.forename: Kuniyasu
authors.initials: K
authors.lastname: Shimokawa
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Seishima
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takami
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D016632:Apolipoprotein A-I / Q000032:analysis* / Q000235:genetics* / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000503:physiopathology*; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D013116:Spinal Cord / Q000737:chemistry* / Q000503:physiopathology*
chemical_list: D016632:Apolipoprotein A-I; D012333:RNA, Messenger