DNA binding properties of oligodeoxynucleotides containing pyrrolidino C-nucleosides.
We have incorporated pyrrolidino-C-nucleosides (pyrrolidino-pseudonucleosides) containing the base uracil and N-1-methyl uracil into oligodeoxynucleotides and compared their thermal duplex and triplex stabilities with unmodified or pseudouridine-containing oligodeoxynucleotides. We find relative destabilizations of triplex formation by ca. -13 to -1 degrees C per modification (relative to thymidine) in a strongly sequence dependent mode. Duplex formation is less destabilizing and more homogeneous with -4 to -2 degrees C per modification.
Häberli
Adrian
A
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, Switzerland.
Leumann
Christian J
CJ
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006358:Hot Temperature; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009705:Nucleosides / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism*
D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D004247:DNA