Extracellular putrescine content after acute excitotoxic brain damage in the rat.
We examined the effects of the local infusion of kainic acid (KA), by reverse dialysis in the rat striatum, on the concentration of polyamines in the extracellular striatal compartment and in tissue. KA infusion markedly increased (3-fold) extracellular putrescine (PUT) concentration, which reached its maximum at the end of the dialysis experiments (6 h). Tissue PUT concentration was also increased (2-fold) in the striatum perfused with KA but not in the contralateral side. Extracellular spermidine (SD) concentration but not tissue SD concentration was affected by KA. The increase in PUT was accompanied by histological damage around the probe and by an increase in ornithine decarboxylase content, as assessed by immunohistochemistry. These results indicate that in the first stages of the excitotoxic lesion, there is an increase in the extracellular concentrations of PUT and SD.
Vivó
Meritxell
M
Department of Neurochemistry, Institut d'Investigacions Biomèdiques de Barcelona, (CSIC-IDIBAPS), c/Rosselló 161, 6th floor, E-08036 Barcelona, Spain.
Camón
Lluísa
L
de Vera
Núria
N
Martínez
Emili
E
D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018690:Excitatory Amino Acid Agonists / Q000009:adverse effects*; D005110:Extracellular Space / Q000378:metabolism*; D002534:Hypoxia, Brain / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007608:Kainic Acid / Q000009:adverse effects; D008297:Male; D011700:Putrescine / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013095:Spermidine / Q000378:metabolism
D018690:Excitatory Amino Acid Agonists; D007608:Kainic Acid; D013095:Spermidine; D011700:Putrescine