title: A non-aphid-transmissible isolate of bean yellow mosaic potyvirus has an altered NAG motif in its coat protein.
abstract: An isolate of Bean yellow mosaic virus (BYMV) not transmitted by aphids (NAT) was compared with the aphid-transmissible isolate (MI) from which it was derived. For each isolate, the sequence of the coat protein and parts of the helper component was determined. A single nucleotide substitution caused a NAG to NAS alteration in the coat protein of the non aphid-transmissible isolate. Loss of aphid transmissibility in isolate BYMV(MI)-NAT was most likely caused by this mutation within the NAG motif. Systemic movement and accumulation of the virus in infected plants were not affected by the mutation.
authors.affiliation: Centre for Legumes in Mediterranean Agriculture, University of Western Australia, Crawley, Australia. wylie@central.murdoch.edu.au
authors.forename: S J
authors.initials: SJ
authors.lastname: Wylie
authors.forename: J
authors.initials: J
authors.lastname: Kueh
authors.forename: B
authors.initials: B
authors.lastname: Welsh
authors.forename: L J
authors.initials: LJ
authors.lastname: Smith
authors.forename: M G K
authors.initials: MG
authors.lastname: Jones
authors.forename: R A C
authors.initials: RA
authors.lastname: Jones
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry*; D007887:Fabaceae / Q000821:virology*; D008969:Molecular Sequence Data; D017800:Potyvirus / Q000737:chemistry*