Prevention and regression of atopic dermatitis by ointment containing NF-kB decoy oligodeoxynucleotides in NC/Nga atopic mouse model.
Atopic dermatitis, a chronic inflammatory skin disease characterized by relapsing eczema and intense prurigo, requires effective and safe pharmacological therapy. In this study, we examined the efficacy of ointment containing NF-kB decoy oligodeoxynucleotides (ODN) on atopic dermatitis lesions in NC/Nga mice, which are characterized by the spontaneous onset of atopic dermatitis in conventional conditions. Topical administration of NF-kB decoy ODN twice a month resulted in a significant reduction in clinical skin condition score and marked improvement of histological findings. Reduction of the atopic skin condition by NF-kB decoy ODN was accompanied by a significant decrease in migration of mast cells into the dermis and an increase in apoptotic cells. Here, we demonstrated the successful treatment of atopic dermatitis with ointment containing NF-kB decoy ODN in a mouse model, promising new therapy for atopic dermatitis.
Nakamura
H
H
Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Suita, Japan.
Aoki
M
M
Tamai
K
K
Oishi
M
M
Ogihara
T
T
Kaneda
Y
Y
Morishita
R
R
D000279:Administration, Cutaneous; D000818:Animals; D003876:Dermatitis, Atopic / Q000276:immunology / Q000628:therapy*; D015316:Genetic Therapy / Q000379:methods*; D020287:In Situ Nick-End Labeling; D008297:Male; D008407:Mast Cells / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D016328:NF-kappa B / Q000235:genetics*; D009824:Ointments; D012869:Skin Absorption; D014162:Transfection / Q000379:methods*
D016328:NF-kappa B; D009824:Ointments