Analysis of oligoguluronic acids with NMR, electrospray ionization-mass spectrometry and high-performance anion-exchange chromatography.
Oligoguluronates were prepared by enzymatic hydrolysis of homopolymeric blocks of guluronic acids. Two different oligosaccharides were prepared by separating final hydrolysates on Q-Sepharose FF ion chromatography. High-performance anion-exchange chromatography analysis showed the high purity of these oligosaccharides. The molecular masses of these two oligosaccharides, determined by electrospray ionization-mass spectrometry, were 396 and 594, respectively. 1H, 13C NMR, H-H COSY and HSQC analysis proved that they were the unsaturated dimer and trimer oligoguluronates. The 1H and 13C NMR chemical shifts of these two oligosaccharides are also reported.
Liu
Yan
Y
Food Science and Technology, Fishery College, Ocean University of Qingdao, China. yanliu@mail.wsu.edu
Jiang
Xiao-Lu
XL
Liao
Wei
W
Guan
Hua-Shi
HS
D000837:Anion Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006603:Hexuronic Acids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000837:Anion Exchange Resins; D006603:Hexuronic Acids; C007896:guluronic acid