[Immunohistochemical detection of hepatitis C virus antigen from hepatocellular carcinoma].
To investigate the infection of HCV and the expression of HCV antigen (HCAg) in HCC, immunohistochemical protocol was performed on tumorous liver tissues from 40 patients with HCC to detect HCAg and hepatitis B virus surface antigen (HBsAg) simultaneously. The results showed that the positive rates for HCAg and HBsAg were 17.5% (7/40) and 70% (28/40) respectively. HBsAg and HCAg were simultaneously detected in six of 40 cases and only one case had HCAg positive alone in the liver tumorous tissue. The positive signals were localized in diffuse cytoplasm of hepatocytes and the positively stained cells were mainly distributed in local pattern. The results suggested that HCV infection did exist in some cases of HCC, so HCV might be a viral risk factor in addition to HBV in the genesis of HCC in China.
Zhou
F
F
Institute of Viral Heaptitis, First Affiliated Hospital, WCUMS, Chengdu 610041.
Zhao
L
L
Liu
C
C
Lei
X
X
Zhou
S
S
Wang
J
J
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000821:virology*; D005260:Female; D016174:Hepacivirus / Q000276:immunology*; D006509:Hepatitis B / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006526:Hepatitis C / Q000821:virology; D018936:Hepatitis C Antigens / Q000032:analysis*; D006801:Humans; D007120:Immunochemistry; D008099:Liver / Q000276:immunology / Q000821:virology; D008113:Liver Neoplasms / Q000821:virology*; D008297:Male; D008875:Middle Aged
D006514:Hepatitis B Surface Antigens; D018936:Hepatitis C Antigens