[Rapid determination of cefazolin in porcine serum by high performance capillary electrophoresis].
A rapid high performance capillary electrophoretic method was established to assay cefazolin in porcine blood serum. Porcine serum was deproteined by 60% acetonitrile and superlants were injected with 10 s pressure load. The final assay condition was: 40 cm x 75 microns silica capillary, 50 mmol/L bicarbonate buffer at pH10, 20 kV for 5 min, 254 nm UV detection. The assay was linear within the range of 5-320 micrograms/ml, the intra-day and inter-day relative standard deviations ranged from 1.7% to 8.0%. Analytical recovery of cefazolin was 107.9%, the limit of detection was 1.5 micrograms/ml, the limit of quantitation was 3.6 micrograms/ml. To investigate the pharmacokinetic profile of cefazolin in pig, six adult pigs were injected with 16 mg/kg cefazolin and all data was analyzed by pharmacokinetic software 3P87. Cefazolin metabolism in adult pigs appeared to be a typical two-compartment model with a clearance of 8.43 ml/(kg.min).
Wei
Q
Q
Lab of Transplant Immunology, First Affiliated Hospital, WCUMS, Chengdu 610041.
Cheng
J
J
Cai
S
S
Peng
Y
Y
Li
S
S
Li
Y
Y
D000818:Animals; D002437:Cefazolin / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods; D013552:Swine
D002437:Cefazolin