title: Highly efficient over-production in E. coli of YvcC, a multidrug-like ATP-binding cassette transporter from Bacillus subtilis.
abstract: ATP-binding cassette (ABC) transporters have often been refractory to over-expression. Using the C41(DE3) E. coli as a host strain, membrane vesicles highly enriched (>50%) in YvcC, a previously uncharacterized ABC transporter from Bacillus subtilis homologous to P-glycoprotein multidrug transporters, were obtained. The functionality of YvcC was assessed by its high vanadate-sensitive ATPase activity and its ability to transport a fluorescent drug, the Hoechst 33342.
authors.affiliation: Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/Université Claude Bernard Lyon I, 7 passage du Vercors, 69367, Lyon, France. jm.jault@ibcp.fr
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Steinfels
authors.forename: Cédric
authors.initials: C
authors.lastname: Orelle
authors.forename: Olivier
authors.initials: O
authors.lastname: Dalmas
authors.forename: François
authors.initials: F
authors.lastname: Penin
authors.forename: Bruno
authors.initials: B
authors.lastname: Miroux
authors.forename: Attilio
authors.initials: A
authors.lastname: Di Pietro
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Jault
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis*; D001562:Benzimidazoles / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D014638:Vanadates
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D001562:Benzimidazoles; D005456:Fluorescent Dyes; D014638:Vanadates; C017807:bisbenzimide ethoxide trihydrochloride