An application of microplate hybridization assay for the confirmation and probe typing of "Norwalk-like viruses".
"Norwalk-like virus" (NLV) genomes are generally detected by using reverse transcription-PCR and confirmed by blot hybridization and nucleotide sequencing because of their fastidious nature. In the present study, the confirmation and typing of NLV genomes were carried out using a streptavidin-biotin binding technique and microplate hybridization assay with digoxigenin labeled probes. Eight probe typing sets (G1A, G1B, G2A, G2B, G2C, G2D, G2E, and G2F) formatted from 6 newly designed probes and 8 probes reported elsewhere were used for hybridization. The correlation between probe typing and nucleotide sequencing was found and our 8 probe sets were useful for the typing of NLVs.
Fukuda
Shinji
S
Division of Microbiology II, Hiroshima Prefectural Institute of Public Health and Environment, Hiroshima, Japan. sfukuda@hicat.ne.jp
Takao
Shinichi
S
Shimazu
Yukie
Y
Miyazaki
Kazuo
K
D017250:Caliciviridae Infections / Q000453:epidemiology / Q000821:virology; D015342:DNA Probes / Q000235:genetics; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology; D006801:Humans; D029322:Norovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D017423:Sequence Analysis, RNA; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D012367:RNA, Viral