Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis.
Resistance to linezolid has been associated with a G2576U mutation in domain V of the 23S rRNA. We analyzed nine clinical isolates of linezolid-resistant enterococci and showed a clear association between the number of 23S rRNA genes containing this mutation and the level of linezolid resistance expressed.
Marshall
S H
SH
Research and Medical Services, Department of Veterans Affairs Medical Center and Division of Infectious Diseases, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
Donskey
C J
CJ
Hutton-Thomas
R
R
Salata
R A
RA
Rice
L B
LB
D000081:Acetamides / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics; D018628:Gene Dosage; D020459:Genes, rRNA; D006801:Humans; D000069349:Linezolid; D009154:Mutation; D023303:Oxazolidinones / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012338:RNA, Ribosomal, 23S / Q000235:genetics
D000081:Acetamides; D023303:Oxazolidinones; D012338:RNA, Ribosomal, 23S; D000069349:Linezolid