In vitro phototoxicity testing: development and validation of a new concentration response analysis software and biostatistical analyses related to the use of various prediction models.
As demonstrated in several validation studies, the dermal phototoxic potential of chemicals in humans can be effectively assessed by in vitro methods. The core of these methods is to monitor dose-response curves of a chemical in the absence and presence of light, to quantify the difference between these two curves by appropriate measures (either the photo-irritancy factor [PIF], or the mean photo effect [MPE]), and to use these measures as predictors of in vivo phototoxicity. We present new concentration-response analysis software for in vitro phototoxicity testing, which runs on current personal computers, and takes into account all the limitations identified when using a former program. We also demonstrate the validity and robustness of this new software by applying it retrospectively to all data available from two phases of the EU/COLIPA validation trial for the 3T3 neutral red update in vitro phototoxicity test. Some frequently raised questions pertaining to the use of prediction models in phototoxicity testing are addressed, including: the necessity of using prediction models based on a cut-off; whether it is justifiable to use sharp prediction cut-off values; whether there is a biostatistical justification for the highest concentration of the test chemical; and whether repeated testing of a chemical is required.
Peters
Björn
B
Humboldt-Universität Berlin, Medizinische Fakultät (Charité), Institut für Biochemie, Monbijoustrasse 2, 10117 Berlin, Germany.
Holzhütter
Hermann-Georg
HG
D016475:3T3 Cells / Q000187:drug effects / Q000378:metabolism; D000826:Animal Testing Alternatives / Q000379:methods*; D000818:Animals; D017484:Dermatitis, Phototoxic; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007509:Irritants / Q000145:classification / Q000633:toxicity; D051379:Mice; D008962:Models, Theoretical; D009499:Neutral Red / Q000378:metabolism; D011237:Predictive Value of Tests; D012984:Software; D012985:Software Design; D015262:Xenobiotics / Q000145:classification / Q000633:toxicity
D007509:Irritants; D015262:Xenobiotics; D009499:Neutral Red