[Epitope mapping of hepatitis C virus non-structure protein 5 from a 7 peptide phage library by using immobilized specific monoclonal antibody].
OBJECTIVE
To screen HCV NS5 mimotopes by using monoclonal antibody and phage peptide library.


METHODS
By using HCV NS5 monoclonal antibody as selective molecule, a 7 peptide phage library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.


RESULTS
Twelve positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was confirmed as the mimotope of HCV NS5.


CONCLUSIONS
HCV mimotope is obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development.
Zhong
Yan Wei
YW
Gene Therapy Research Center, Institute of infectious Diseases, 302th Hospital of PLA, Beijing 100039, China.
Cheng
Jun
J
Wang
Gang
G
Tian
Xiao Jun
XJ
Chen
Xinhua
X
Liu
Yan
Y
Li
Li
L
Zhang
Ling Xia
LX
Chen
Ju Mei
JM
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D006526:Hepatitis C / Q000628:therapy; D019151:Peptide Library; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology; D012324:RNA-Dependent RNA Polymerase
D000911:Antibodies, Monoclonal; D019151:Peptide Library; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase