The effect of long-term supplementation of vitamin C on leukocyte adhesion to the cerebral endothelium in STZ-induced diabetic rats.
The effect of long-term supplementation of vitamin C on leukocyte adhesion to the cerebral endothelium was investigated in streptozotocin (STZ)-induced diabetic rats. Diabetes was induced in male Wistar Furth rats by intravenous injection of STZ. The vitamin C, ascorbic acid, was supplemented with drinking water (1 g/l). The rats were divided into control and diabetic groups without or with supplementation of vitamin C. The cerebral microcirculation was directly observed through a cranial window after different periods (12, 24 and 36 weeks) of vitamin C supplementation, using fluorescence videomicroscopy. Leukocyte adhesion to the venular endothelium was examined by labeling leukocytes with rhodamin 6G. The number density of adherent leukocytes in STZ-diabetic rats was increased significantly, compared with control rats. This increase in leukocyte adhesion was prevented by the long-term supplemented vitamin C. It was suggested that the antioxidant effect of vitamin C might be responsible for the prevention of leukocyte adhesion in diabetes mellitus.
Jariyapongskul
Amporn
A
Department of Physiology, Faculty of Medicine, Srinakharinvirot University, Bangkok 10110, Thailand.
Patumraj
Suthiluk
S
Yamaguchi
Saburo
S
Niimi
Hideyuki
H
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001921:Brain / Q000098:blood supply* / Q000166:cytology; D002448:Cell Adhesion / Q000187:drug effects; D002560:Cerebrovascular Circulation; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000178:diet therapy*; D019587:Dietary Supplements; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects*; D008833:Microcirculation; D018715:Microscopy, Video; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
D013311:Streptozocin; D001205:Ascorbic Acid