title: Oral administration of recombinant adeno-associated virus elicits human immunodeficiency virus-specific immune responses.
abstract: Oral vaccines can induce both systemic and mucosal immunity. Mucosal immunity, especially regional cell-mediated immunity, plays an important role in protecting individuals from infectious diseases such as acquired immunodeficiency syndrome. In this study, a recombinant adeno-associated virus vector expressing human immunodeficiency virus type 1 env gene (AAV-HIV) was orally administered to BALB/c mice. Systemic and regional immunity was induced in the mice. Furthermore, the immunization significantly reduced viral load after an intrarectal challenge with a recombinant vaccinia virus expressing HIV env gene. Moreover, we also show that dendritic cells might contribute to the AAV-HIV vector-induced immune responses.
authors.affiliation: Department of Bacteriology, Yokohama City University School of Medicine, Yokohama 236-0004, Japan.
authors.forename: Ke-Qin
authors.initials: KQ
authors.lastname: Xin
authors.forename: Takaaki
authors.initials: T
authors.lastname: Ooki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Mizukami
authors.forename: Kenji
authors.initials: K
authors.lastname: Hamajima
authors.forename: Koji
authors.initials: K
authors.lastname: Okudela
authors.forename: Kumiko
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Kojima
authors.forename: Nao
authors.initials: N
authors.lastname: Jounai
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Kumamoto
authors.forename: Shin
authors.initials: S
authors.lastname: Sasaki
authors.forename: Dennis
authors.initials: D
authors.lastname: Klinman
authors.forename: Keiya
authors.initials: K
authors.lastname: Ozawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Okuda
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000235:genetics; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control*; D000284:Administration, Oral; D000818:Animals; D000229:Dependovirus; D015686:Gene Products, env / Q000235:genetics* / Q000276:immunology; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D018928:Immunity, Mucosal / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics
chemical_list: D016915:AIDS Vaccines; D015686:Gene Products, env; D014614:Vaccines, Synthetic