Rapid detection of the hypertension-associated A1166C polymorphism of the angiotensin II type 1 receptor (AGTR1).
Screening for polymorphisms in the human type 1 angiotensin II receptor locus (AGTR1) has led to the identification of an A1166C transversion in the 3'-untranslated region. This molecular variant, C(1166), has been linked to essential hypertension. We describe here a rapid method for the detection of this point mutation by a simple modification of PCR amplification with allele-specific oligonucleotides (ASO), so as to avoid a hybridization procedure involving either radioactive- or non-radioactive-labeled probes, labeled primers, or restriction typing. The procedure described is convenient for routine clinical laboratory use with manual sample processing and offers the potential for further automation, as well.
Stankovic
Aleksandra
A
VINCA Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics, Belgrade, Yugoslavia.
Alavantic
Dragan
D
D001483:Base Sequence; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin / Q000235:genetics*
D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin