Palgin sensitizes the adriamycin-induced apoptosis via the enhancement of Fas/Fas ligand expression.
This study was designed to evaluate the synergistic cytotoxicity of herb prescription, Palgin, in adriamycin-treated cancer cells. The combination of Palgin and adriamycin synergistically augmented the cytotoxicity of Chang and HL-60 cells, but not in Hep3B and Alexander cells. The cytotoxicity of two drugs was revealed as apoptosis characterized by nuclear fragmentation. The apoptotic cell death was accompanied by the activation of caspase-3 as well as cleavage of poly(ADP) ribose polymerase (PARP) in Chang cells. Interestingly, a synergistic increase in apoptosis by the combination of two drugs was accompanied by the enhancement of Fas and Fas ligand (FasL) expression in Chang cells. Taken together, the combination of Palgin and adriamycin significantly augmented the apoptotic cytotoxicity of Fas-positive cells, such as Chang and HL-60 cells, via activation of caspase signaling pathway. This notion will provide a new trial to treat cancer patients in clinical fields as a complementary treatment of Western and Oriental medicine.
Lim
Yun-Sook
YS
Professional Graduate School of Oriental Medicine, Wonkwang University, 344-2 Shinyong-dong, Iksan, Jeonbuk 570-749, South Korea.
So
Hong-Seob
HS
Kim
Myung-Sunny
MS
Moon
Gu
G
Won
Jin-Hee
JH
Baek
Seung-Wha
SW
Moon
Sun-Rock
SR
Yang
Sei-Hoon
SH
Kim
Byoung-Ju
BJ
Ko
Chang-Bo
CB
Park
Raekil
R
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology*; D004789:Enzyme Activation; D053222:Fas Ligand Protein; D018922:HL-60 Cells; D006801:Humans; D006868:Hydrolysis; D008562:Membrane Glycoproteins / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism*
D002843:Chromatin; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D010936:Plant Extracts; D019014:fas Receptor; C467627:palgin; D004317:Doxorubicin; D011065:Poly(ADP-ribose) Polymerases; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases