title: Expression of Chicken Smooth Muscle Myosin Light Chain Kinase in NIH 3T3 Cells.
abstract: The myosin light chain kinase (MLCK) is important in regulating the smooth muscle contraction. The pBKrsv-MLCK was obtained by inserting MLCK cDNA into pBKrsv polylinker and transfected into NIH 3T3 cells. The results revealed that the transfected cells can express chicken MLCK identified by DNA-PCR, RT-PCR and Western blot. The expressed MLCK has high activity, thus providing the basis for further investigation of the signal transduction and regulation of smooth muscle contraction of MLCK.
authors.affiliation: Department of Biochemistry and Molecular Biology Laboratory, Anhui Medical University, Hefei 230032, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Shu-Yu
authors.initials: SY
authors.lastname: Gui
authors.forename: D.M.
authors.initials: DM
authors.lastname: Watterson