title: Gene sequence and the 1.8 A crystal structure of the tungsten-containing formate dehydrogenase from Desulfovibrio gigas.
abstract: Desulfovibrio gigas formate dehydrogenase is the first representative of a tungsten-containing enzyme from a mesophile that has been structurally characterized. It is a heterodimer of 110 and 24 kDa subunits. The large subunit, homologous to E. coli FDH-H and to D. desulfuricans nitrate reductase, harbors the W site and one [4Fe-4S] center. No small subunit ortholog containing three [4Fe-4S] clusters has been reported. The structural homology with E. coli FDH-H shows that the essential residues (SeCys158, His159, and Arg407) at the active site are conserved. The active site is accessible via a positively charged tunnel, while product release may be facilitated, for H(+) by buried waters and protonable amino acids and for CO(2) through a hydrophobic channel.
authors.affiliation: REQUIMTE/CQFB, Departamento de Química, FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Hans
authors.initials: H
authors.lastname: Raaijmakers
authors.forename: Sofia
authors.initials: S
authors.lastname: Macieira
authors.forename: João M
authors.initials: JM
authors.lastname: Dias
authors.forename: Susana
authors.initials: S
authors.lastname: Teixeira
authors.forename: Sergey
authors.initials: S
authors.lastname: Bursakov
authors.forename: Robert
authors.initials: R
authors.lastname: Huber
authors.forename: José J G
authors.initials: JJ
authors.lastname: Moura
authors.forename: Isabel
authors.initials: I
authors.lastname: Moura
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Romão
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002245:Carbon Dioxide / Q000378:metabolism; D018360:Crystallography, X-Ray; D003901:Desulfovibrio / Q000201:enzymology* / Q000235:genetics; D004583:Electrons; D005560:Formate Dehydrogenases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D011522:Protons; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity; D013329:Structure-Activity Relationship; D014414:Tungsten / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; D011522:Protons; D002245:Carbon Dioxide; D005560:Formate Dehydrogenases; D014414:Tungsten