Liquid chromatographic resolution of gemifloxacin mesylate on a chiral stationary phase derived from crown ether.
A new racemic fluoroquinolone antibacterial agent, gemifloxacin mesylate, has been successfully resolved on a chiral stationary phase (CSP) derived from (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid. Compared to the Crownpak CR(+) column, the CSP used in this study was more effective for the resolution of racemic gemifloxacin mesylate, especially in terms of analytical time. The resolution of gemifloxacin mesylate enantiomers on the CSP was found to be dependent on the type and content of organic and acidic modifiers in the aqueous mobile phase and the column temperature.
Hyun
Myung Ho
MH
Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Pusan 609-735, Korea. mhhyun@pusan.ac.kr
Han
Sang Cheol
SC
Cho
Yoon Jae
YJ
Jin
Jong Sung
JS
Lee
Wonjae
W
D000890:Anti-Infective Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004988:Ethers, Cyclic / Q000737:chemistry*; D024841:Fluoroquinolones; D000077735:Gemifloxacin; D009287:Naphthyridines / Q000032:analysis*; D013237:Stereoisomerism
D000890:Anti-Infective Agents; D004988:Ethers, Cyclic; D024841:Fluoroquinolones; D009287:Naphthyridines; D000077735:Gemifloxacin