Incorporation of a novel nucleobase allows stable oligonucleotide-directed triple helix formation at the target sequence containing a purine.pyrimidine interruption.
Thermal denaturation experiments have established that an oligonucleotide incorporating the artificial nucleobase S, does form a stable triplex with a double stranded DNA which exhibits a pyrimidine interruption within the oligopurine sequence.
Guianvarc'h
D
D
Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse 91198. Gif-sur-Yvette, France.
Benhida
R
R
Fourrey
J L
JL
Maurisse
R
R
Sun
J S
JS
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism*; D011687:Purines / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism
D009841:Oligonucleotides; D011687:Purines; D011743:Pyrimidines; C051802:triplex DNA; D004247:DNA