title: OcaB is required for normal transcription and V(D)J recombination of a subset of immunoglobulin kappa genes.
abstract: OcaB, a transcriptional coactivator also known as Bob-1 or OBF-1, was isolated on the basis of its ability to enhance transcription of immunoglobulin (Ig) genes in vitro. Paradoxically, OcaB(-/-) mice showed no apparent deficiency in Ig gene transcription, only cellular immune defects including absence of germinal centers (GC) and decreased numbers of immature B cells; the genes targeted by OcaB were not determined. Here we report that OcaB is essential for V(D)J recombination of a subset of Igkappa genes. We show that OcaB modulates recombination by directly enhancing Igkappa gene transcription in vivo.
authors.affiliation: Laboratory of Molecular Immunology, New York, NY 10021, USA.
authors.forename: Rafael
authors.initials: R
authors.lastname: Casellas
authors.forename: Mila
authors.initials: M
authors.lastname: Jankovic
authors.forename: Gesa
authors.initials: G
authors.lastname: Meyer
authors.forename: Anna
authors.initials: A
authors.lastname: Gazumyan
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.forename: Robert
authors.initials: R
authors.lastname: Roeder
authors.forename: Michel
authors.initials: M
authors.lastname: Nussenzweig
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D005803:Genes, Immunoglobulin; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D015534:Trans-Activators / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D007145:Immunoglobulin kappa-Chains; C490424:Pou2af1 protein, mouse; D015534:Trans-Activators