title: Purification and Characterization of Phospholipase A(2) from the Venom of Snake Trimeresurus stejnegeri Schmidt.
abstract: A phospholipase A(2)(PLA(2)) was purified from the venom of the snake Trimeresurus stejnegeri Schmidt by Sephadex G-75 gel filtration, Mono Q ion exchange chromatography and Superose-12 gel filtration with FPLC and proved to be homogeneous as shown in SDS-PAGE and IEF. Its molecular weight is around 18 000 and isoelectric point is 4.7. Amino acid composition of PLA(2) was determined. Apart from hydrolyzing phosphatidylcholine, the enzyme has a potent inhibitory effect on platelet aggregation induced by ADP or collagen with half-inhibitory concentration of 10-15 &mgr;g/ml and 50-80 &mgr;g/ml respectively.
authors.affiliation: Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.forename: Wei-Jia
authors.initials: WJ
authors.lastname: Wu
authors.forename: Rong
authors.initials: R
authors.lastname: Qian
authors.forename: Ke-Yi
authors.initials: KY
authors.lastname: Wang
authors.forename: Yuan-Cong
authors.initials: YC
authors.lastname: Zhou