title: The interference of insulin antibodies in insulin immunometric assays.
abstract: We investigated the interference of insulin antibodies in two insulin immunometric assays (Bio-Rad and Elecsys) by measuring direct and free insulin in plasma from 30 patients without insulin antibodies (group 1), as screened by a sensitive radio-binding assay, and in plasma from 80 patients with insulin antibodies (group 2). In group 1, the direct/free insulin ratio did not differ from 1, showing the equivalence of free and direct insulin results in theses samples. In group 2, this ratio was markedly increased (mean: Bio-Rad 2.63, Elecsys 5.02) and correlated positively with the insulin antibody radio-binding assay result (r=0.92 for the correlation between Bio-Rad and Elecsys assays after log-transformation of the ratios). In samples containing insulin antibodies, direct insulin concentration was frequently lower than total (bound and unbound) insulin measured with the Bio-Rad and Elecsys assays. This study underlines the interference of insulin antibodies in insulin immunometric assays and the importance of assessing an insulin immunometric assay for sensitivity towards the presence of these antibodies.
authors.affiliation: Unité d'Analyses Endocriniennes, CNRS UMR 7004, Université Louis Pasteur, Faculté de Médecine, Strasbourg, France. sapin@ipb.u-strasbg.fr
authors.forename: Rémy
authors.initials: R
authors.lastname: Sapin
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D003920:Diabetes Mellitus / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D003951:Diagnostic Errors; D006801:Humans; D007118:Immunoassay / Q000592:standards*; D007328:Insulin / Q000097:blood / Q000276:immunology*; D011869:Radioligand Assay
chemical_list: D001323:Autoantibodies; D007328:Insulin