title: Drosophila yolk protein produced in E. coli is accumulated by mosquito ovaries.
abstract: Despite similar functions, the yolk proteins of the higher dipteran flies and the vitellogenins found in other insects are unrelated at the sequence level and have evolved from different genes. Both are selectively endocytosed into the ovary via receptors belonging to the LDLR receptor subfamily. We cloned the Drosophila yp1 gene into an E. coli expression vector and showed that the yolk protein produced by E. coli is taken up into ovaries of both Drosophila melanogaster and the malaria mosquito Anopheles gambiae, which normally uses vitellogenin.
authors.affiliation: Institute of Cell and Molecular Biology, The University of Edinburgh, Edinburgh, UK. Mary.Bownes@ed.ac.uk
authors.forename: M
authors.initials: M
authors.lastname: Bownes
authors.forename: H
authors.initials: H
authors.lastname: Hurd
authors.forename: T
authors.initials: T
authors.lastname: Büsgen
authors.forename: D
authors.initials: D
authors.lastname: Servay
authors.forename: S
authors.initials: S
authors.lastname: Alvis
authors.forename: B
authors.initials: B
authors.lastname: Popovic
authors.forename: S
authors.initials: S
authors.lastname: Bruce
authors.forename: I
authors.initials: I
authors.lastname: Burns
authors.forename: K
authors.initials: K
authors.lastname: Rothwell
authors.forename: Malcolm
authors.initials: M
authors.lastname: Walkinshaw
mesh_terms: D000818:Animals; D000852:Anopheles / Q000378:metabolism*; D003001:Cloning, Molecular; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors; D010053:Ovary / Q000378:metabolism; D014819:Vitellogenins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D014819:Vitellogenins; C105973:YP1 protein, Drosophila