title: [Cloning of AE1-c-end cDNA and construction of its expression plasmid for yeast two-hybrid system].
abstract: In this study, about 350 bp cDNA fragment was amplified by PCR. After being sequenced, the AE1-c-end gene fragment was cloned into EcoR I-Pst I site of pGADT7 to form AD ends in the yeast two-hybrid system. The recombinant plasmid was transformed into yeast AH109, and the expression in the yeast was observed. The results demonstrate that AE1-c-end was obtained. pGADT7-AE1-c-end has no toxic effect on the yeast. It can serve as a target gene of yeast two-hybrid system.
authors.affiliation: Department of Pathophysiology, Harbin Medical University, Harbin 150086.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Li
authors.forename: Guohui
authors.initials: G
authors.lastname: Fu
authors.forename: Yong
authors.initials: Y
authors.lastname: Qin
authors.forename: Hongqing
authors.initials: H
authors.lastname: Du
authors.forename: Xiaoshu
authors.initials: X
authors.lastname: Jiang
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Xiangang
authors.initials: X
authors.lastname: Kong
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary; D010957:Plasmids; D016133:Polymerase Chain Reaction; D020798:Two-Hybrid System Techniques; D015003:Yeasts
chemical_list: D018076:DNA, Complementary