title: Trypanosoma cruzi H+-ATPase 1 (TcHA1) and 2 (TcHA2) genes complement yeast mutants defective in H+ pumps and encode plasma membrane P-type H+-ATPases with different enzymatic properties.
abstract: Previous studies in Trypanosoma cruzi have shown that intracellular pH homeostasis requires ATP and is affected by H(+)-ATPase inhibitors, indicating a major role for ATP-driven proton pumps in intracellular pH control. In the present study, we report the cloning and sequencing of a pair of genes linked in tandem (TcHA1 and TcHA2) in T. cruzi which encode proteins with homology to fungal and plant P-type proton-pumping ATPases. The genes are expressed at the mRNA level in different developmental stages of T. cruzi: TcHA1 is expressed maximally in epimastigotes, whereas TcHA2 is expressed predominantly in trypomastigotes. The proteins predicted from the nucleotide sequence of the genes have 875 and 917 amino acids and molecular masses of 96.3 and 101.2 kDa, respectively. Full-length TcHA1 and an N-terminal truncated version of TcHA2 complemented a Saccharomyces cerevisiae strain deficient in P-type H(+)-ATPase activity, the proteins localized to the yeast plasma membrane, and ATP-driven proton pumping could be detected in proteoliposomes reconstituted from plasma membrane purified from transfected yeast. The reconstituted proton transport activity was reduced by inhibitors of P-type H(+)-ATPases. C-terminal truncation did not affect complementation of mutant yeast, suggesting the lack of C-terminal autoinhibitory domains in these proteins. ATPase activity in plasma membrane from TcHA1- and (N-terminal truncated) TcHA2-transfected yeast was inhibited to different extents by vanadate, whereas the latter yeast strain was more resistant to extremes of pH, suggesting that the native proteins may serve different functions at different stages in the T. cruzi life cycle.
authors.affiliation: Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana-Champaign, 61802, USA.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Luo
authors.forename: David A
authors.initials: DA
authors.lastname: Scott
authors.forename: Roberto
authors.initials: R
authors.lastname: Docampo
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000378:metabolism; D017353:Gene Deletion; D015723:Gene Library; D005816:Genetic Complementation Test; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D017434:Protein Structure, Tertiary; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000235:genetics*; D011522:Protons; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000201:enzymology*; D014638:Vanadates / Q000494:pharmacology
chemical_list: D005656:Fungal Proteins; D011522:Protons; D015800:Protozoan Proteins; D012333:RNA, Messenger; D014638:Vanadates; D000255:Adenosine Triphosphate; C469751:HA1 protein, Trypanosoma cruzi; C469752:HA2 protein, Trypanosoma cruzi; D006180:Proton-Translocating ATPases; D005947:Glucose