title: Abscisic Acid Structure-Activity Relationships in Barley Aleurone Layers and Protoplasts (Biological Activity of Optically Active, Oxygenated Abscisic Acid Analogs).
abstract: Optically active forms of abscisic acid (ABA) and their oxygenated metabolites were tested for their biological activity by examining the effects of the compounds on the reversal of gibberellic acid-induced [alpha]-amylase activity in barley (Hordeum vulgare cv Himalaya) aleurone layers and the induction of gene expression in barley aleurone protoplasts transformed with a chimeric construct containing the promoter region of an albumin storage protein gene. Promotion of the albumin storage protein gene response had a more strict stereochemical requirement for elicitation of an ABA response than inhibition of [alpha]-amylase gene expression. The naturally occurring stereoisomer of ABA and its metabolites were more effective at eliciting an ABA-like response. ABA showed the highest activity, followed by 7[prime]-hydroxyABA, with phaseic acid being the least active. Racemic 8[prime]-hydroxy-2[prime],3[prime]-dihydroABA, an analog of 8[prime]-hydroxyABA, was inactive, whereas racemic 2[prime],3[prime]-dihydroABA was as effective as ABA. The differences in response of the same tissue to the ABA enantiomers lead us to conclude that there exists more than one type of ABA receptor and/or multiple signal transduction pathways in barley aleurone tissue.
authors.affiliation: Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2 (R.D.H., J.-H.L., D.D.).
authors.forename: R. D.
authors.initials: RD
authors.lastname: Hill
authors.forename: J. H.
authors.initials: JH
authors.lastname: Liu
authors.forename: D.
authors.initials: D
authors.lastname: Durnin
authors.forename: N.
authors.initials: N
authors.lastname: Lamb
authors.forename: A.
authors.initials: A
authors.lastname: Shaw
authors.forename: S. R.
authors.initials: SR
authors.lastname: Abrams