Identification and characterization of a gene coding a novel isoform of DEAD-box protein.
A gene coding a novel isoform of DEAD-box protein named testicular DEAD-box protein (tDbp), presumably involved in testicular function, was identified and characterized. Testicular DEAD-box protein was cloned from a human testis cDNA library. The cDNA microarray hybridization showed that it was expressed at a higher level in adult testis than in embryo testis. Reverse transcription-polymerase chain reaction indicated that tDbp was specifically expressed in testis, but not in some other tissues.
Yin
Lanlan
L
Key Laboratory of Reproductive Medicine, Nanjing Medical University, PR China.
Li
JianMin
J
Zhu
Hu
H
Lin
Min
M
Cheng
Lijun
L
Wang
Youqun
Y
Zhou
Zuomin
Z
Sha
Jiahao
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D053487:DEAD-box RNA Helicases; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics*; D029543:Nucleocytoplasmic Transport Proteins; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics*; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development
D018076:DNA, Complementary; D009687:Nuclear Proteins; D029543:Nucleocytoplasmic Transport Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C121148:DDX19B protein, human; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases