[The features and significance of immunophenotyping in adult acute leukemia].
Immunophenotypic assay was determined by using indirect immunofluorescent method in 120 adult patients with acute leukemia. Eight monoclonal CD antibodies consisting of anti-CD2, -CD7, -CD10, -CD19, -CD13, -CD33, -CD14, and anti-HLA-DR monoclonal antibodies were applied conventionally. The results were that immunophenotyping had high corresponding rate to FAB morphology typing in diagnosing acute leukemia. Also there were some patients who had cross or inverse CD marker expression or no specific CD antigen expression. There were some immunophenotypic features in AML-M3, ALL-L3, and B-Lineage and T-Lineage acute lymphocytic leukemia respectively. Prognostic significance was observed in the patients with specific immunophenotypes, that is acute myeloid leukemia patients with lymphoid antigen (Ly+ AML) and some cases with inverse immunophenotypes.
Pei
M F
MF
Research Laboratory of Hematology, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
Zhang
G S
GS
Xiao
L
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D018941:Antigens, CD19 / Q000276:immunology; D018826:CD13 Antigens / Q000276:immunology; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D015470:Leukemia, Myeloid, Acute / Q000276:immunology*; D015473:Leukemia, Promyelocytic, Acute / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015260:Neprilysin / Q000276:immunology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology*
D018941:Antigens, CD19; D018826:CD13 Antigens; D015260:Neprilysin