Biotransformation of p-xylene and 2,6-dimethylnaphthalene by xylene monooxygenase cloned from a Sphingomonas isolate.
Sphingomonas strain ASU1 was isolated from an industrial wastewater bioreactor and grew on 2,6-dimethylnaphthalene (2,6-DMN) as the sole carbon/energy source. The genes for a xylene monooxygenase were cloned from strain ASU1. Expression of the ASU1 xylene monooxygenase was compared to expression of the pWWO xylene monooxygenase in Escherichia coli. Both monooxygenases transformed p-xylene and 2,6-DMN by initially hydroxylating one methyl group. In addition, the ASU1 monooxygenase also hydroxylated the second methyl group on p-xylene and 2,6-DMN whereas the pWWO monooxygenase hydroxylated the second methyl group only on p-xylene. Endogenous E. coli enzymes contributed to further oxidation of the resulting aromatic alcohols to form aromatic carboxylates.
Bramucci
M
M
Central Research and Development, DuPont Company, PO Box 80328, Wilmington, DE 19880-0328, USA. Michael.G.Bramucci@usa.dupont.com
Singh
M
M
Nagarajan
V
V
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D007220:Industrial Waste; D009281:Naphthalenes / Q000378:metabolism*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D020578:Sphingomonas / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D014992:Xylenes / Q000378:metabolism*
D004269:DNA, Bacterial; D007220:Industrial Waste; D009281:Naphthalenes; D012722:Sewage; D014992:Xylenes; C031286:4-xylene; C028519:2,6-dimethylnaphthalene; D010105:Oxygenases; C067624:xylene monooxygenase