A non-aphid-transmissible isolate of bean yellow mosaic potyvirus has an altered NAG motif in its coat protein.
An isolate of Bean yellow mosaic virus (BYMV) not transmitted by aphids (NAT) was compared with the aphid-transmissible isolate (MI) from which it was derived. For each isolate, the sequence of the coat protein and parts of the helper component was determined. A single nucleotide substitution caused a NAG to NAS alteration in the coat protein of the non aphid-transmissible isolate. Loss of aphid transmissibility in isolate BYMV(MI)-NAT was most likely caused by this mutation within the NAG motif. Systemic movement and accumulation of the virus in infected plants were not affected by the mutation.
Wylie
S J
SJ
Centre for Legumes in Mediterranean Agriculture, University of Western Australia, Crawley, Australia. wylie@central.murdoch.edu.au
Kueh
J
J
Welsh
B
B
Smith
L J
LJ
Jones
M G K
MG
Jones
R A C
RA
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry*; D007887:Fabaceae / Q000821:virology*; D008969:Molecular Sequence Data; D017800:Potyvirus / Q000737:chemistry*