Universal hybridization using LNA (locked nucleic acid) containing a novel pyrene LNA nucleotide monomer.
A novel pyrene LNA nucleotide monomer is shown to mediate universal hybridization when incorporated into a DNA strand or a 2'-OMe-RNA/LNA chimeric strand. For the latter, high-affinity universal hybridization without compromising the base-pairing selectivity of bases neighbouring the universal pyrene LNA nucleotide monomer is documented.
Babu
B R
BR
Department of Chemistry, University of Southern Denmark, DK-5230 Odense M, Denmark.
Wengel
J
J
D020029:Base Pairing; D004247:DNA / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D013696:Temperature
D009696:Nucleic Acids; D009711:Nucleotides; D011721:Pyrenes; D012313:RNA; D004247:DNA