Oral administration of recombinant adeno-associated virus elicits human immunodeficiency virus-specific immune responses.
Oral vaccines can induce both systemic and mucosal immunity. Mucosal immunity, especially regional cell-mediated immunity, plays an important role in protecting individuals from infectious diseases such as acquired immunodeficiency syndrome. In this study, a recombinant adeno-associated virus vector expressing human immunodeficiency virus type 1 env gene (AAV-HIV) was orally administered to BALB/c mice. Systemic and regional immunity was induced in the mice. Furthermore, the immunization significantly reduced viral load after an intrarectal challenge with a recombinant vaccinia virus expressing HIV env gene. Moreover, we also show that dendritic cells might contribute to the AAV-HIV vector-induced immune responses.
Xin
Ke-Qin
KQ
Department of Bacteriology, Yokohama City University School of Medicine, Yokohama 236-0004, Japan.
Ooki
Takaaki
T
Mizukami
Hiroaki
H
Hamajima
Kenji
K
Okudela
Koji
K
Hashimoto
Kumiko
K
Kojima
Yoshitsugu
Y
Jounai
Nao
N
Kumamoto
Yasuko
Y
Sasaki
Shin
S
Klinman
Dennis
D
Ozawa
Keiya
K
Okuda
Kenji
K
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000235:genetics; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control*; D000284:Administration, Oral; D000818:Animals; D000229:Dependovirus; D015686:Gene Products, env / Q000235:genetics* / Q000276:immunology; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D018928:Immunity, Mucosal / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics
D016915:AIDS Vaccines; D015686:Gene Products, env; D014614:Vaccines, Synthetic