Lipid peroxidation by nitric oxide supplements after spinal cord injury: effect of antioxidants in rats.
To determine the extent to which exogenous nitric oxide (NO) might affect hemodynamics and/or increase oxidative damage after acute spinal cord (SC) injury, rats were submitted to SC contusion, and given a NO donor or NO precursor. Intravenous isosorbide dinitrate (10 microg/kg per min) or L-arginine (300 mg/kg per 23 h) showed a tendency to increase lipid peroxidation (LP), although without reaching significance compared to non-treated injured rats 24 h post-injury, and without affecting mean arterial pressure and heart rate importantly. LP due to injury and exogenous NO was significantly inhibited by the co-administration of a cocktail of antioxidants (12 mg/kg superoxide dismutase mimetic, 27000 U/kg catalase, and 12 mg/kg glutathione), but less effectively for the injury-L-arginine condition. These results demonstrate that in order to further test the potential neuroprotective effect of NO enhancing reagents after SC injury, antioxidants must be included in the treatment scheme.
Santoscoy
Carlos
C
Research Unit for Neurological Diseases, IMSS, Tlalpan 4430, Mexico City 14050, Mexico.
Ríos
Camilo
C
Franco-Bourland
Rebecca E
RE
Hong
Enrique
E
Bravo
Guadalupe
G
Rojas
Guillermo
G
Guízar-Sahagún
Gabriel
G
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D051381:Rats; D020318:Rats, Long-Evans; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism
D000975:Antioxidants; D020030:Nitric Oxide Donors; D009569:Nitric Oxide