title: Crystal structure of the cytoskeleton-associated protein glycine-rich (CAP-Gly) domain.
abstract: Cytoskeleton-associated proteins (CAPs) are involved in the organization of microtubules and transportation of vesicles and organelles along the cytoskeletal network. A conserved motif, CAP-Gly, has been identified in a number of CAPs, including CLIP-170 and dynactins. The crystal structure of the CAP-Gly domain of Caenorhabditis elegans F53F4.3 protein, solved by single wavelength sulfur-anomalous phasing, revealed a novel protein fold containing three beta-sheets. The most conserved sequence, GKNDG, is located in two consecutive sharp turns on the surface, forming the entrance to a groove. Residues in the groove are highly conserved as measured from the information content of the aligned sequences. The C-terminal tail of another molecule in the crystal is bound in this groove.
authors.affiliation: Southeast Collaboratory for Structural Genomics, University of Georgia, Athens 30602, USA.
authors.forename: Songlin
authors.initials: S
authors.lastname: Li
authors.forename: Jim
authors.initials: J
authors.lastname: Finley
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Shi-Hong
authors.initials: SH
authors.lastname: Qiu
authors.forename: Hongli
authors.initials: H
authors.lastname: Chen
authors.forename: Chi-Hao
authors.initials: CH
authors.lastname: Luan
authors.forename: Mike
authors.initials: M
authors.lastname: Carson
authors.forename: Jun
authors.initials: J
authors.lastname: Tsao
authors.forename: David
authors.initials: D
authors.lastname: Johnson
authors.forename: Guangda
authors.initials: G
authors.lastname: Lin
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Willie
authors.initials: W
authors.lastname: Thomas
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Nagy
authors.forename: Bingdong
authors.initials: B
authors.lastname: Sha
authors.forename: Lawrence J
authors.initials: LJ
authors.lastname: DeLucas
authors.forename: Bi-Cheng
authors.initials: BC
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D003598:Cytoskeletal Proteins / Q000737:chemistry*; D005998:Glycine / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D029742:Caenorhabditis elegans Proteins; D003598:Cytoskeletal Proteins; D005998:Glycine