Correlation of phylogenetic lineages of group B Streptococci, identified by analysis of restriction-digestion patterns of genomic DNA, with infB alleles and mobile genetic elements.
Phylogenetic lineages of pathogenic Streptococcus agalactiae (group B streptococci [GBS]) can be identified by analysis of restriction-digestion patterns (RDPs) of chromosomal DNA. The purpose of the present study was to correlate GBS RDP types and (1) alleles of the highly conserved gene encoding translation-initiation factor IF2, infB, and/or (2) the inserted elements IS1548 and GBSi1. Only 1 combination of serotype and infB allele was found within each RDP type. Strains within a particular RDP type also tend to have the same inserted elements in each of 3 loci examined. A novel insertion sequence, designated "IS1563," was found within all RDP type II-2 strains. Most RDP types could be identified by a combination of serotype, infB allele, and inserted elements at each of the loci. These molecular markers can be used to identify GBS populations and to correlate RDP types and phylogenetic lineages identified by different methods.
Takahashi
Shinji
S
Division of Microbiology, Joshi-Eiyoh University, Chiyoda, Sakado, Saitama, Japan.
Detrick
Shauna
S
Whiting
April A
AA
Blaschke-Bonkowksy
Anne J
AJ
Aoyagi
Youko
Y
Adderson
Elisabeth E
EE
Bohnsack
John F
JF
D000483:Alleles; D015373:Bacterial Typing Techniques; D015342:DNA Probes / Q000032:analysis; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D039881:Prokaryotic Initiation Factor-2 / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D013290:Streptococcal Infections / Q000382:microbiology*; D013292:Streptococcus agalactiae / Q000145:classification* / Q000235:genetics
D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005819:Genetic Markers; D039881:Prokaryotic Initiation Factor-2