title: Development of a frozen cell array as a high-throughput approach for cell-based analysis.
abstract: Recent advances in molecular biology, human genetics, and functional genomics tremendously increase the number of molecular targets available for potential therapeutic and diagnostic use. To complement DNA array data, cost-efficient high-throughput technologies providing reliable information at the protein level need to be developed. Here we describe the generation of a frozen cell array that required the use of single cell suspensions and could serve various applications such as the analysis of specific antibody or ligand binding to a large panel of different cell types. As an example, binding of an anti-human epithelial cell adhesion molecule monoclonal antibody to 24 different cell lines has been analyzed using the cell array and compared to the data generated by fluorescence-activated cell sorting. The reliability and flexibility of our frozen cell array technology is compatible with the needs of high-throughput screening for drug discovery and target validation.
authors.affiliation: Department of Assay and Automation Technology, South San Francisco, California 94080, USA. stephanj@gene.com
authors.forename: Jean Philippe
authors.initials: JP
authors.lastname: Stephan
authors.forename: Silvia
authors.initials: S
authors.lastname: Schanz
authors.forename: Anne
authors.initials: A
authors.lastname: Wong
authors.forename: Peter
authors.initials: P
authors.lastname: Schow
authors.forename: Wai Lee T
authors.initials: WL
authors.lastname: Wong
mesh_terms: D000906:Antibodies / Q000032:analysis; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules / Q000032:analysis; D002460:Cell Line; D015925:Cryopreservation; D003584:Cytological Techniques; D000071858:Epithelial Cell Adhesion Molecule; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule