title: Absence of simian virus 40 in human brain tumors from northern India.
abstract: Simian virus 40 (SV40), a monkey polyomavirus, was a contaminant of early poliovirus vaccines administered to millions of individuals in the 1950s and early 1960s. SV40 causes brain tumors in laboratory animals, and SV40 DNA sequences have been variably identified in human choroid plexus tumors and ependymomas. We studied the possible association between SV40 and human brain tumors in northern India, where humans have frequent contact with SV40-infected rhesus macaques. DNA from pathologic specimens from 33 ependymomas, 14 choroid plexus tumors and 18 control brain tissues (contused brain, brain metastases) was extracted and analyzed under masked conditions. We used real-time PCR to detect and quantify SV40 (T antigen) and human (GAPDH) DNA sequences. The SV40 PCR assay detected as few as 10 copies of SV40 DNA and had a linear range from 1 x 10(2) to 1 x 10(6) copies. SV40 DNA was detected in 1 specimen (an ependymoma). However, few SV40 DNA copies were detected in this sample (<10 copies, equivalent to <1 copy/350 cells, based on simultaneous GAPDH quantification), and SV40 was not detected when this sample was retested. Our findings do not support a role for SV40 in choroid plexus tumors or ependymomas from northern India.
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, MD 20852, USA. engelse@exchange.nih.gov
authors.forename: Eric A
authors.initials: EA
authors.lastname: Engels
authors.forename: Chitra
authors.initials: C
authors.lastname: Sarkar
authors.forename: Richard W
authors.initials: RW
authors.lastname: Daniel
authors.forename: Patti E
authors.initials: PE
authors.lastname: Gravitt
authors.forename: Kusum
authors.initials: K
authors.lastname: Verma
authors.forename: Martha
authors.initials: M
authors.lastname: Quezado
authors.forename: Keerti V
authors.initials: KV
authors.lastname: Shah
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001921:Brain / Q000821:virology; D001932:Brain Neoplasms / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D016545:Choroid Plexus Neoplasms / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D004340:Drug Contamination; D004806:Ependymoma / Q000821:virology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D007194:India; D008253:Macaca mulatta / Q000821:virology; D009693:Nucleic Acid Hybridization; D023321:Poliovirus Vaccines; D016133:Polymerase Chain Reaction; D013539:Simian virus 40 / Q000235:genetics
chemical_list: D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D023321:Poliovirus Vaccines; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases