Microgravity modifies protein kinase C isoform translocation in the human monocytic cell line U937 and human peripheral blood T-cells.
Individual protein kinase C (PKC) isoforms fulfill distinct roles in the regulation of the commitment to differentiation, cell cycle arrest, and apoptosis in both monocytes and T-cells. The human monocyte like cell line U937 and T-cells were exposed to microgravity, during spaceflight and the translocation (a critical step in PKC signaling) of individual isoforms to cell particulate fraction examined. PKC activating phorbol esters induced a rapid translocation of several PKC isoforms to the particulate fraction of U937 monocytes under terrestrial gravity (1 g) conditions in the laboratory. In microgravity, the translocation of PKC beta II, delta, and epsilon in response to phorbol esters was reduced in microgravity compared to 1 g, but was enhanced in weak hypergravity (1.4 g). All isoforms showed a net increase in particulate PKC following phorbol ester stimulation, except PKC delta which showed a net decrease in microgravity. In T-cells, phorbol ester induced translocation of PKC delta was reduced in microgravity, compared to 1 g, while PKC beta II translocation was not significantly different at the two g-levels. These data show that microgravity differentially alters the translocation of individual PKC isoforms in monocytes and T-cells, thus providing a partial explanation for the modifications previously observed in the activation of these cell types under microgravity.
Hatton
Jason P
JP
Laboratory of Cell Growth, Veterans Affairs Medical Center, Mail Code 151F, San Francisco, California 94121, USA. hattonjasonp@aol.com
Gaubert
Francois
F
Cazenave
Jean-Pierre
JP
Schmitt
Didier
D
D015153:Blotting, Western; D005434:Flow Cytometry; D006801:Humans; D008954:Models, Biological; D009000:Monocytes / Q000166:cytology*; D010703:Phorbol Esters / Q000494:pharmacology; D020033:Protein Isoforms; D011493:Protein Kinase C / Q000737:chemistry* / Q000378:metabolism*; D021381:Protein Transport; D013026:Space Flight; D013601:T-Lymphocytes / Q000166:cytology*; D013997:Time Factors; D020298:U937 Cells; D014893:Weightlessness
D010703:Phorbol Esters; D020033:Protein Isoforms; D011493:Protein Kinase C