title: Three-dimensional quantitative structure-activity relationships of mazindol analogues at the dopamine transporter.
abstract: A three-dimensional quantitative structure-activity relationship (3D-QSAR) study was performed on a series of mazindol analogues using the comparative molecular field analysis (CoMFA) method with their corresponding binding affinities for the displacement of [(3)H]WIN 35 428 from rat caudate putamen tissue. The cross-validated CoMFA models were derived from a training set of 50 compounds, and the predictive ability of the resulting CoMFA models was evaluated against a test set of 21 compounds. A set of alignment rules was derived to superimpose these compounds onto a template structure, mazindol (1). These CoMFA models yielded significant cross-validated r(2)(cv) values. Inclusion of additional descriptors did not improve the significance of the CoMFA models; thus, steric and electrostatic fields are the relevant descriptors for these compounds. The best QSAR model was selected on the basis of the predictive ability of the activity on the external test set of compounds. The analysis of coefficient contour maps provided further insight into the binding interactions of mazindol analogues with the DAT. The aromatic rings C and D are involved in hydrophobic interactions in which ring D may bind in a large hydrophobic groove. The relative orientation of these two rings is also important for high binding affinity to the DAT.
authors.affiliation: Medicinal Chemistry Section, National Institute on Drug Abuse-Intramural Research Program/NIH, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA.
authors.forename: Santosh S
authors.initials: SS
authors.lastname: Kulkarni
authors.forename: Amy Hauck
authors.initials: AH
authors.lastname: Newman
authors.forename: William J
authors.initials: WJ
authors.lastname: Houlihan
mesh_terms: D000818:Animals; D001665:Binding Sites; D004298:Dopamine / Q000378:metabolism*; D050483:Dopamine Plasma Membrane Transport Proteins; D018765:Dopamine Uptake Inhibitors / Q000737:chemistry* / Q000378:metabolism; D066298:In Vitro Techniques; D008454:Mazindol / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D009419:Nerve Tissue Proteins; D011699:Putamen / Q000378:metabolism; D021281:Quantitative Structure-Activity Relationship; D011869:Radioligand Assay; D051381:Rats
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; D018765:Dopamine Uptake Inhibitors; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; C493216:Slc6a3 protein, rat; D008454:Mazindol; D004298:Dopamine