title: The responsiveness of human V delta 1 gamma delta T cells to Borrelia burgdorferi is largely restricted to synovial-fluid cells from patients with Lyme arthritis.
abstract: It has been shown that human gamma delta T cells expressing the V delta 1 T cell receptor (TCR) respond to the spirochete Borrelia burgdorferi. Lysates of 3 Borrelia genospecies triggered the proliferation of peripheral-blood mononuclear cells not only from patients with skin manifestations of Borrelia infection and from patients with Lyme arthritis but also from healthy donors. However, with the exception of 1 patient with Lyme arthritis, no selective expansion of V delta 1-expressing gamma delta T cells was induced. In contrast, synovial-fluid mononuclear cells (SFMC) from 3 of 5 patients with Lyme arthritis responded with a selective outgrowth of V delta 1 gamma delta T cells. V delta 1 gamma delta T cell lines established from SFMC coexpressed various TCR V gamma chains, although V gamma 8 was preferentially used. Thus, the responsiveness to Borrelia antigens is not a general property of V delta 1-expressing gamma delta T cells but is largely restricted to V delta 1 gamma delta T cells recruited into the inflamed tissue.
authors.affiliation: Institute of Immunology, University of Kiel, Kiel, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Glatzel
authors.forename: Frank
authors.initials: F
authors.lastname: Entschladen
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Zollner
authors.forename: Peter
authors.initials: P
authors.lastname: Kraiczy
authors.forename: Volker
authors.initials: V
authors.lastname: Brade
authors.forename: Roland
authors.initials: R
authors.lastname: Kaufmann
authors.forename: Ottmar
authors.initials: O
authors.lastname: Janssen
authors.forename: Beate
authors.initials: B
authors.lastname: Lengl-Janssen
authors.forename: Daniela
authors.initials: D
authors.lastname: Wesch
authors.forename: Dieter
authors.initials: D
authors.lastname: Kabelitz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001170:Arthritis, Infectious / Q000276:immunology*; D025065:Borrelia burgdorferi / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008193:Lyme Disease / Q000276:immunology*; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D013582:Synovial Fluid / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta