title: Factors influencing cDNA microarray hybridization on silylated glass slides.
abstract: cDNA microarray technology is becoming the technique of choice for studying gene expression and gene expression patterns. Although experimental protocols are available, only limited methodological information on microarray manufacture, hybridization, and signal interpretation has been published. The aim of this paper is to provide more insight into the practical aspects of microarray construction and hybridization. The influence of the size, composition, and concentration of the spotted DNA fragments on the final hybridization signal and the effect of hybridization volume, sample concentration, and sample depletion have been tested and are discussed.
authors.affiliation: RIKILT (State Institute for Quality Control of Agricultural Products), Gene Expression and Detection Centre, P.O. Box 230, 6700 AE Wageningen, The Netherlands. n.l.w.franssen@rikilt.wag-ur.nl
authors.forename: Nicole L W
authors.initials: NL
authors.lastname: Franssen-van Hal
authors.forename: Oscar
authors.initials: O
authors.lastname: Vorst
authors.forename: Evelien
authors.initials: E
authors.lastname: Kramer
authors.forename: Robert D
authors.initials: RD
authors.lastname: Hall
authors.forename: Jaap
authors.initials: J
authors.lastname: Keijer
mesh_terms: D000588:Amines / Q000737:chemistry; D001482:Base Composition; D018938:Caco-2 Cells; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005822:Genetic Vectors / Q000378:metabolism; D005898:Glass / Q000737:chemistry*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D032306:Petunia / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D000588:Amines; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D012821:Silanes; D011061:Poly A; D008156:Luciferases