title: YIL113w encodes a functional dual-specificity protein phosphatase which specifically interacts with and inactivates the Slt2/Mpk1p MAP kinase in S. cerevisiae.
abstract: We show here that the YIL113w gene of Saccharomyces cerevisiae encodes a functional protein phosphatase. Yil113p shows no activity in vitro towards either phosphorylated casein or myelin basic protein. However, Yil113p dephosphorylates activated extracellular signal-regulated kinase 2 MAP kinase indicating that it is a dual-specificity MAP kinase phosphatase. In support of this we find that Yil113p specifically interacts with the stress-activated Slt2/Mpk1p MAP kinase of S. cerevisiae. Furthermore, expression of Yil113p causes the dephosphorylation of Slt2/Mpk1p in vivo, while expression of an inactive mutant of Yil113p causes the accumulation of phosphorylated Slt2/Mpk1p. We conclude that the physiological target of YIL113p is Slt2/Mpk1p.
authors.affiliation: Cancer Research UK, Molecular Pharmacology Unit, Biomedical Research Centre, Ninewells Hospital, DD1 9SY, Dundee, UK
authors.forename: Michelle
authors.initials: M
authors.lastname: Collister
authors.forename: Mark P
authors.initials: MP
authors.lastname: Didmon
authors.forename: Fiona
authors.initials: F
authors.lastname: MacIsaac
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Stark
authors.forename: Neil Q
authors.initials: NQ
authors.lastname: MacDonald
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Keyse
mesh_terms: D054637:Dual-Specificity Phosphatases; D004789:Enzyme Activation; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism*; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D020798:Two-Hybrid System Techniques
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D020928:Mitogen-Activated Protein Kinases; C072724:SLT2 protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; D054637:Dual-Specificity Phosphatases; C467054:SDP1 protein, S cerevisiae