title: The Isolation of Carbohydrate-binding Peptide from Scarlet Runner Bean Lectin.
abstract: The carbohydrate-binding peptide fragment of scarlet runner bean (Phaseolus coccineus var. rubronanus) lectin has been prepared by trypsin digestion. The carbohydrate-binding peptide was isolated from digested solution by affinity chromatography on thyroglobulin-Sepharose column, Bio-Gel P-4 gel filtration column and reverse phase HPLC on C-8 column. The fraction of peak I from HPLC which bound specifically with Man(8)GlcNAc(2) was demonstrated by using dot blot technique with [(3)H]- Man(8)GlcNAc(2).
authors.affiliation: Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Wei-Xing
authors.initials: WX
authors.lastname: Shi
authors.forename: Jin-Fang
authors.initials: JF
authors.lastname: Song
authors.forename: Ce
authors.initials: C
authors.lastname: Sun