title: Changes of interleukin expression correlate with Helicobacter pylori infection and lymph node metastases in gastric carcinoma.
abstract: Helicobacter pylori (HP) infection induces expression of IL-8 and IL-10 in benign gastric epithelium. This study compared the expression of cytokines in CD4+ and CD8+ lymphocyte subsets of peripheral blood lymphocytes (PBL), benign mucosal lymphocytes (ML), and tumor infiltrative lymphocytes (TIL) as well as in the benign and malignant epithelial cells of the same patient, with respect to the presence of HP infection, lymph node metastases, and tumor histologic type. The mRNA of the cytokines was measured by a semiquantitative RT-PCR method. The levels were ranked and compared using the Wilcoxon sign-ranked test. Compared with CD8+ ML, the CD8+ TIL expresses higher levels of IL-6 and IL-8 but lower level of IL-4 in patients with lymph node metastases. In patients with HP infection, expression of IL-8 and IL-10 was higher in the gastric carcinoma cells than in the benign epithelial cells while expression of IL-6 and IL-8 were higher in CD8+ TIL than CD8+ ML. Overexpression of IL-8 in HP associated gastric carcinomas suggested that they might have arisen from HP-infected epithelial cells.
authors.affiliation: Institute of Digestive Diseases, 4th Military University, Xian, PR China.
authors.forename: Xiao Y
authors.initials: XY
authors.lastname: Zhang
authors.forename: Wing Y
authors.initials: WY
authors.lastname: Chan
authors.forename: Bruce M
authors.initials: BM
authors.lastname: Whitney
authors.forename: Dai M
authors.initials: DM
authors.lastname: Fan
authors.forename: John H S
authors.initials: JH
authors.lastname: Chow
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Enders K W
authors.initials: EK
authors.lastname: Ng
authors.forename: Sheung C S
authors.initials: SC
authors.lastname: Chung
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000556:secondary / Q000821:virology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D016481:Helicobacter Infections / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D016131:Lymphocyte Subsets / Q000378:metabolism / Q000473:pathology; D016246:Lymphocytes, Tumor-Infiltrating / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000821:virology
chemical_list: D017931:DNA Primers; D007378:Interleukins; D012333:RNA, Messenger