title: Partial VP1/2A gene sequence based molecular epidemiology of wild type 1 poliovirus isolates from some parts of India.
abstract: Genomic variability within the sequences of VP1/2A junction among polioviruses from across the globe has revealed the existence of several endemic genotypes and their epidemiological inter-relationships; but such data on Indian isolates are scanty. The present work was intended to ascertain the persistence and transmission pattern of different genotypes of wild type 1 polioviruses circulating in India. Forty-eight wild type 1 poliovirus isolates obtained from different parts of India during 1996-8 were subjected to RT-PCR and nucleotide sequencing using M13 tailed primers. A 293 base pair region was amplified and sequenced for genetic variation study. Considering the 15% divergence of the sequences from Sabin 1, the isolates from six different states of India confirmed a single dominant genotype 4. Phylogenetic analysis revealed the circulation and active inter-state transmission of many genetically distinct strains of wild poliovirus type 1 belonging to genotype 4. This warrants the need for insisting on more efficient surveillance mechanisms so as to assess the impact of an extensive pulse polio immunization programme in India.
authors.affiliation: Division of Biochemistry and Biotechnology and Microbiology, National Institute of Communicable Diseases, Delhi, India.
authors.forename: R
authors.initials: R
authors.lastname: Anand
authors.forename: D
authors.initials: D
authors.lastname: Ghosh
authors.forename: A V
authors.initials: AV
authors.lastname: Bhupatiraju
authors.forename: S
authors.initials: S
authors.lastname: Broor
authors.forename: S T
authors.initials: ST
authors.lastname: Pasha
authors.forename: S
authors.initials: S
authors.lastname: Khare
authors.forename: M
authors.initials: M
authors.lastname: Kumar
authors.forename: K K
authors.initials: KK
authors.lastname: Dutta
authors.forename: A
authors.initials: A
authors.lastname: Rai
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D011051:Poliomyelitis / Q000517:prevention & control / Q000635:transmission; D017955:Poliovirus / Q000145:classification / Q000235:genetics*; D014764:Viral Proteins
chemical_list: D036022:Capsid Proteins; C442540:VP1 protein, Poliovirus; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; C057400:picornain 2A, Picornavirus