title: Differential sensitivity of Jurkat and primary T cells to caspase-independent cell death triggered upon Fas stimulation.
abstract: Fas-induced apoptosis, a key event in the regulation of lymphocyte homeostasis, is mainly mediated by the activation of a cascade of caspases. Using caspase- and Fas-associated death domain protein-deficient Jurkat cell lines as well as a pan-caspase inhibitor (Z-VAD-fmk), we observed a second Fas-induced cell death event, independent of any known caspase activation. This pathway is of slow kinetics and displays some features of necrosis. However, this caspase-independent pathway does not seem to play a significant role in the Fas-mediated death of primary activated T cell blasts. Indeed, in this setting, Fas-induced cell death was always substantially inhibited by Z-VAD-fmk, suggesting that caspase activation is an absolute requirement in the Fas-induced death of primary human T lymphocytes.
authors.affiliation: INSERM U429, Développement Normal et Pathologique du Système Immunitaire, Hôpital Necker-Enfants Malades, Paris, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Vonarbourg
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Stolzenberg
authors.forename: Eliska
authors.initials: E
authors.lastname: Hölzelova
authors.forename: Alain
authors.initials: A
authors.lastname: Fischer
authors.forename: Françoise Le
authors.initials: FL
authors.lastname: Deist
authors.forename: Frédéric
authors.initials: F
authors.lastname: Rieux-Laucat
mesh_terms: D017209:Apoptosis; D020169:Caspases / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D007700:Kinetics; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000502:physiology*; D019014:fas Receptor / Q000502:physiology*
chemical_list: D019014:fas Receptor; D020169:Caspases