Age-dependent response to insecticides and enzymatic variation in susceptible and resistant codling moth larvae.
Insecticide resistance in the codling moth, Cydia pomonella, partly results from increased metabolic detoxification. The aim of this study was to follow the age variations in larval susceptibility to deltamethrin and teflubenzuron in one susceptible (S) strain, and two resistant (Rv and Rt) ones selected for resistance to deltamethrin and diflubenzuron, respectively. The age variation of the activities of cytochrome P450-dependent monooxygenase (MFO), glutathione S-transferases (GST), and esterases in S and both resistant strains were simultaneously investigated. The highest levels of insecticide resistance were recorded in late instars in both resistant strains, although Rv neonates exhibited enhanced resistance to deltamethrin. The involvement of an additional deltamethrin-specific mechanism of resistance, which could be mainly expressed in early instars, was supported by previous demonstration of a kdr point mutation in the Rv strain. The cross-resistance between deltamethrin and teflubenzuron indicated the involvement of non-specific metabolic pathways in resistance to teflubenzuron, rather than target site modification. A positive correlation between enhanced GST activities and deltamethrin resistance suggested that this mechanism might take place into the adaptive response of C. pomonella to pyrethroids treatments. Enhanced MFO activity was recorded in each instar of the two resistant strains compared to the susceptible one. But these activities were not correlated to the responses to deltamethrin nor to teflubenzuron. In the light of these findings, studying age-dependence of responses to selection is central to the implementation of monitoring tests of resistances, especially if the target instars are difficult to collect in the field.
Bouvier
Jean-Charles
JC
I.N.R.A., U.M.R. Ecologie des Invertébrés, Avignon, France. jbouvier@avignon.inra.fr
Boivin
Thomas
T
Beslay
Dominique
D
Sauphanor
Benoît
B
D000367:Age Factors; D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D001681:Biological Assay; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D007605:Juvenile Hormones / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D009036:Moths / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D009570:Nitriles; D011722:Pyrethrins / Q000494:pharmacology*
D001549:Benzamides; D007306:Insecticides; D007605:Juvenile Hormones; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase; D004950:Esterases; C052595:teflubenzuron