Evaluation of antioxidative and mutagenic properties of 50% ethanolic extract from red beans fermented by Aspergillus oryzae.
Various bean products fermented by microorganisms are commonly consumed in Asian diets; however, the safety or functional properties of fermented beans can vary with different microbial species and with different processes being applied to different beans. The objectives of this study were to evaluate the antioxidative and mutagenic properties of 50% ethanolic extracts from red beans fermented by Aspergillus oryzae. The extracts' antioxidative activities, including alpha,alpha;-diphenyl-beta-picryl-hydrazyl (DPPH) radical-scavenging effects, Fe(2+)-chelating ability, and reducing power, were studied in vitro. The antioxidative effects provided by the extracts depended strongly on their concentrations. In general, antioxidative activity increased with extract concentration to a certain point and then leveled off as the concentration further increased. The fermented red bean extracts showed less of a scavenging effect on the DPPH radical and less reducing power than the commercial antioxidants alpha-tocopherol and butylated hydroxytoluene, but better Fe(2+)-chelating ability. No mutagenicity or toxicity effect on any of the tested strains (Salmonella Typhimurium TA97, TA98, TA100, TA102, and TA1535) was found for the 50% ethanolic extracts of fermented red beans with the Ames mutagenicity assay. These results suggest that the 50% ethanolic extracts were not mutagenic.
Chou
Su-Tze
ST
Department of Food and Nutrition, Providence University, Shalu, Taiwan, Republic of China.
Chang
Cheng-Tien
CT
Chao
Wen-Wan
WW
Chung
Yun-Chin
YC
D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D001236:Aspergillus oryzae / Q000378:metabolism*; D001713:Biphenyl Compounds; D000431:Ethanol; D007887:Fabaceae / Q000382:microbiology*; D005285:Fermentation; D005296:Ferrous Compounds / Q000737:chemistry; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D007502:Iron Chelating Agents / Q000494:pharmacology; D009152:Mutagenicity Tests; D010084:Oxidation-Reduction; D010851:Picrates; D010936:Plant Extracts
D000975:Antioxidants; D001713:Biphenyl Compounds; D005296:Ferrous Compounds; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D007502:Iron Chelating Agents; D010851:Picrates; D010936:Plant Extracts; D000431:Ethanol; C004931:1,1-diphenyl-2-picrylhydrazyl