Expression patterns of antioxidant proteins in brains of patients with sporadic Creutzfeldt-Jacob disease.
Using two-dimensional gel electrophoresis (2-DE) and Western blot analysis, we were able to identify and quantify six antioxidant proteins, peroxiredoxin (Prx) I, Prx II, Prx III, 1-Cys Prx, putative peroxisomal antioxidant enzyme (PLP), and mitochondrial Mn superoxide dismutase (Mn-SOD) in two individual brain regions, cerebellum and frontal cortex of patients with sporadic Creutzfeldt-Jacob (sCJD). Among six antioxidant proteins, 1-Cys Prx showed significant increase (P > 0.05) in sCJD frontal cortex whereas Prx I was decreased (P > 0.01). In cerebellum, levels of all antioxidant proteins studied were comparable to those of controls. Our findings provide evidence for the link between aberrant expression of antioxidant proteins, 1-Cys Prx and Prx I and CJD neuropathogenesis and we discuss the neuropathological meaning of these dysregulated antioxidant proteins in sCJD brain.
Krapfenbauer
Kurt
K
F. Hoffmann-La Roche Ltd, Pharmaceuticals Division, Basel, Switzerland.
Yoo
Byong Chul
BC
Fountoulakis
Michael
M
Mitrova
Eva
E
Lubec
Gert
G
D000368:Aged; D000975:Antioxidants / Q000302:isolation & purification; D015153:Blotting, Western; D016022:Case-Control Studies; D007562:Creutzfeldt-Jakob Syndrome / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000302:isolation & purification*; D010544:Peroxidases / Q000235:genetics / Q000302:isolation & purification; D054464:Peroxiredoxins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000975:Antioxidants; D009419:Nerve Tissue Proteins; D010544:Peroxidases; D054464:Peroxiredoxins