title: Analytical electron microscopy as a tool for accessing colloid formation process in natural waters.
abstract: Analytical electron microscopy was used to characterize aquatic iron-rich colloids. We focused our attention on a redox transition medium in the drainage water of a peat soil. In the anoxic peat water, observations by transmission electron microscopy and associated energy dispersive analyses (TEM-EDS) highlight the presence of spherical entities (approximately 100-600 nm), containing only traces of iron. The increase of dissolved oxygen concentration favours the formation of iron oxy(hydr)oxides. In the oxygenated drain, particles with the same morphology and size range are present. Statistical TEM-EDS analyses show that they represent the only colloidal form of iron in the drain samples. Nevertheless, although Fe-K peaks appear clearly on EDS spectra, the proportion of iron in these colloids reaches at most 4% at. (whereas C + O > 90% at.). Structural information completes this study. Both electron spectroscopic imaging (ESI) and electron energy-loss spectroscopy (EELS) reveal the disparity between element distributions within the drain entities. Iron and calcium are preferably distributed on the outer sphere of the particle, whereas carbon and oxygen follow the theoretical variation of the signal intensity within a plain sphere. The implication of organic matter as nucleation site for iron precipitation is spectacularly demonstrated by the presence of nanometre-sized iron-rich phases highlighted by EELS line scans.
authors.affiliation: Institut de Chimie Minérale et Analytique, Université de Lausanne, CH-1015 Lausanne, Switzerland. christine.mondi@lmcp.jussieu.fr
authors.forename: C
authors.initials: C
authors.lastname: Mondi
authors.forename: K
authors.initials: K
authors.lastname: Leifer
authors.forename: D
authors.initials: D
authors.lastname: Mavrocordatos
authors.forename: D
authors.initials: D
authors.lastname: Perret
mesh_terms: D003102:Colloids / Q000737:chemistry*; D004583:Electrons; D008854:Microscopy, Electron / Q000379:methods*; D014867:Water / Q000737:chemistry*
chemical_list: D003102:Colloids; D014867:Water