[Spectrophotometric determination of copper in the hair of the patients with rheumatoid arthritis].
A new color reaction of copper (II) with solochrome cyanine R was studied by spectrophotometry. The results were that in the pH6 of HAc-NaAc buffer solution solochrome cyanine R reacted with copper (II) to form a violet complex; the maximum absorption of the complex was at 565 nm and its apparent molar absorption coefficient was 8.25 x 10(5) L.mol-1.cm-1. Beer's Law was obeyed in the range of 0-0.48 microgram.ml-1. This method was used to determine copper levels in the hair samples of 5 normal persons and 5 rheumatoid arthritis patients. The results showed that the hair copper level of arthritis patients was significantly higher than that of normal persons (P < 0.01).
Cheng
X Y
XY
Department of Chemistry, Hunan Medical University, Changsha 410078.
Li
Z H
ZH
D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D001557:Benzenesulfonates; D003300:Copper / Q000032:analysis*; D006197:Hair / Q000737:chemistry; D006801:Humans; D013053:Spectrophotometry / Q000379:methods
D001557:Benzenesulfonates; C010332:solochrome cyanine R; D003300:Copper