"Stemness": transcriptional profiling of embryonic and adult stem cells.
The transcriptional profiles of mouse embryonic, neural, and hematopoietic stem cells were compared to define a genetic program for stem cells. A total of 216 genes are enriched in all three types of stem cells, and several of these genes are clustered in the genome. When compared to differentiated cell types, stem cells express a significantly higher number of genes (represented by expressed sequence tags) whose functions are unknown. Embryonic and neural stem cells have many similarities at the transcriptional level. These results provide a foundation for a more detailed understanding of stem cell biology.
Ramalho-Santos
Miguel
M
Department of Molecular and Cellular Biology and Howard Hughes Medical Institute (HHMI), Harvard University, Cambridge, MA 02138, USA.
Yoon
Soonsang
S
Matsuzaki
Yumi
Y
Mulligan
Richard C
RC
Melton
Douglas A
DA
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D002454:Cell Differentiation; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology*; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D020547:Lateral Ventricles / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology*; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D015203:Reproducibility of Results; D015398:Signal Transduction; D013234:Stem Cells / Q000502:physiology*; D014158:Transcription, Genetic
D018528:ATP-Binding Cassette Transporters; D004265:DNA Helicases