title: [Effect of thrombin on cell proliferation and plasminogen activator inhibitor type (PAI-1) expression in human embryonic glomerular mesangial cells].
abstract: OBJECTIVE
  To explore the effect of thrombin on cell proliferation and plasminogen-activator inhibitor Type 1(PAI-1) expression in human embryonic glomerular mesangial cells.
  
  
  METHODS
  Methyl-tetragolium (MTT) incorporation, fibrin plate assay and Northern blotting hybridization were used to examine the mesangial cells proliferation, protein activity and mRNA expression of PAI-1 in cultured human embryonic mesangial cells induced by thrombin, respectively.
  
  
  RESULTS
  Thrombin enhanced glomerular mesangial cell proliferation, PAI-1 protein activity and mRNA expression in a dose-dependent manner, which could be blocked by hirudin, a specific inhibitor of thrombin.
  
  
  CONCLUSIONS
  Thrombin could enhance mesangial cell proliferation, and up-regulate protein activity and mRNA expression of PAI-1 in cultured human embryonic glomerular mesangial cells. It may play an important role in the pathogenesis of glomerulosclerosis induced by thrombin.
authors.affiliation: Department of Pediatrics, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
authors.forename: Q N
authors.initials: QN
authors.lastname: He
authors.forename: Z W
authors.initials: ZW
authors.lastname: Yi
authors.forename: X M
authors.initials: XM
authors.lastname: Chen
authors.forename: B
authors.initials: B
authors.lastname: Fu
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005333:Fetus; D005920:Glomerular Mesangium / Q000166:cytology*; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013917:Thrombin / Q000494:pharmacology*
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D013917:Thrombin