Roles of uracil-DNA glycosylase and dUTPase in virus replication.
Herpesviruses and poxviruses are known to encode the DNA repair enzyme uracil-DNA glycosylase (UNG), an enzyme involved in the base excision repair pathway that specifically removes the RNA base uracil from DNA, while at least one retrovirus (human immunodeficiency virus type 1) packages cellular UNG into virus particles. In these instances, UNG is implicated as being important in virus replication. However, a clear understanding of the role(s) of UNG in virus replication remains elusive. Herpesviruses, poxviruses and some retroviruses encode dUTPase, an enzyme that can minimize the misincorporation of uracil into DNA. The encoding of dUTPase by these viruses also implies their importance in virus replication. An understanding at the molecular level of how these viruses replicate in non-dividing cells should provide clues to the biological relevance of UNG and dUTPase function in virus replication.
Chen
Renxiang
R
Ohio State University Biochemistry Graduate Program, Ohio State University, USA2.|Department of Molecular Virology, Immunology, and Medical Genetics, Center for Retrovirus Research, and Comprehensive Cancer Center, Ohio State University Medical Center, 2078 Graves Hall, 333 West 10th Ave, Columbus, OH 43210, USA1.
Wang
Huating
H
Molecular, Cellular, and Developmental Biology Graduate Program, Ohio State University, USA3.|Department of Molecular Virology, Immunology, and Medical Genetics, Center for Retrovirus Research, and Comprehensive Cancer Center, Ohio State University Medical Center, 2078 Graves Hall, 333 West 10th Ave, Columbus, OH 43210, USA1.
Mansky
Louis M
LM
Molecular, Cellular, and Developmental Biology Graduate Program, Ohio State University, USA3.|Ohio State University Biochemistry Graduate Program, Ohio State University, USA2.|Department of Molecular Virology, Immunology, and Medical Genetics, Center for Retrovirus Research, and Comprehensive Cancer Center, Ohio State University Medical Center, 2078 Graves Hall, 333 West 10th Ave, Columbus, OH 43210, USA1.
D000818:Animals; D045647:DNA Glycosylases; D004261:DNA Replication / Q000502:physiology*; D016336:Gene Products, vpr / Q000235:genetics / Q000502:physiology; D015497:HIV-1 / Q000235:genetics / Q000502:physiology; D006564:Herpesviridae / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D006801:Humans; D016086:Lentivirus / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D011212:Poxviridae / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D011755:Pyrophosphatases / Q000378:metabolism*; D051981:Uracil-DNA Glycosidase; D014779:Virus Replication / Q000502:physiology*; D054325:vpr Gene Products, Human Immunodeficiency Virus
D016336:Gene Products, vpr; D054325:vpr Gene Products, Human Immunodeficiency Virus; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase