Multiple gammac-receptor expression in adult T-cell leukemia.
Constitutive expression of the IL-2 receptor (IL-2R) on adult T-cell leukemia (ATL) cells and the presence of permanent IL-2-dependent ATL cell lines indicate that the signal transduction system via IL-2R is a key element for the development of this disease. IL-2R is a member of the common gamma-chain (gammac)-receptor family and shares gamma with IL-4R, IL-7R, IL-9R, and IL-15R. In addition to IL-2R, ATL cells express IL-15R and respond to IL-15. In the present study, we examined other members of this receptor family. ATL cells showed various levels of IL-4Ralpha (CD124) and IL-7Ralpha (CD127) expression, and responded to these cytokines. In contrast, ATL cells hardly responded to IL-9. As primary samples were a mixed population and the results may have been modified by contaminating normal cells, we used ATL cell lines as pure ATL cell populations. Here, we report that IL-2-dependent ATL cell lines also express IL-4Ralpha and respond to IL-4, which was verified by the activation of cytoplasmic transcriptional activator Stat6 protein. Moreover, a novel ATL cell line that grows stably in an IL-7-dependent manner was established from one of the cell lines, and IL-7 induced Stat5 activation in this cell line. These results indicated that ATL cells have the potential to express all gammac-receptors except IL-9R. Overlapping and switching of cytokine receptors supported the idea that ATL cells can rapidly select the appropriate gammac-receptor according to conditions.
Baba
Hirofumi
H
Department of Laboratory Medicine, Nagasaki University School of Medicine, Nagasaki, Japan.
Yamada
Yasuaki
Y
Mori
Naoki
N
Hayashibara
Toshihisa
T
Harasawa
Hitomi
H
Tsuruda
Kazuto
K
Sugahara
Kazuyuki
K
Soda
Hiroshi
H
Takasaki
Yumi
Y
Tawara
Masayuki
M
Hirakata
Yoichi
Y
Tomonaga
Masao
M
Kamihira
Shimeru
S
D000328:Adult; D015703:Antigens, CD / Q000235:genetics; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D015851:Interleukin-7 / Q000494:pharmacology; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000235:genetics; D019948:Receptors, Interleukin-4 / Q000235:genetics; D020395:Receptors, Interleukin-7 / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D007376:Interleukin-2; D015851:Interleukin-7; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015375:Receptors, Interleukin-2; D019948:Receptors, Interleukin-4; D020395:Receptors, Interleukin-7; D015847:Interleukin-4