Phylogenetic analysis and in situ identification of "Nostocoida limicola"-like filamentous bacteria in activated sludge from industrial wastewater treatment plants.
The diversity of filamentous bacteria present in industrial wastewater treatment plants was analysed by a combination of classical and molecular-biological approaches. Many unknown filamentous bacteria were observed in about 80 screened activated sludge samples from different industries with sometimes severe bulking sludge problems. A special focus was paid to filaments which resembled "Nostocoida limicola", a filamentous bacterium which was found to be present in many WWTPs. These filamentous bacteria are hardly cultivable and only one strain was obtained and maintained in co-culture with a yeast. The 16S rRNA sequences of several other "Nostocoida limicola"-like filamentous bacteria from different sludge samples were obtained by micromanipulation and different molecular-biological methods. The sequences were phylogenetically analyzed and specific molecular probes were developed and applied. The results clearly demonstrate that "Nostocoida limicola"-like filaments from industrial WWTPs are different from all other "Nostocoida limicola" types investigated so far. Our strains are affiliated to the alpha-subclass of Proteobacteria.
Snaidr
I
I
Vermicon AG, München, Germany.
Beimfohr
C
C
Levantesi
C
C
Rossetti
S
S
van der Waarde
J
J
Geurkink
B
B
Elkelboom
D
D
Lemaitre
M
M
Tandoi
V
V
D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D007220:Industrial Waste; D010802:Phylogeny; D020560:Proteobacteria / Q000302:isolation & purification / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid
D017931:DNA Primers; D004269:DNA, Bacterial; D007220:Industrial Waste; D012722:Sewage