Prostaglandin F2alpha-induced nest-building behaviour is associated with increased hypothalamic c-fos and c-jun mRNA expression.
Intramuscular injection of the naturally occurring prostaglandin F2alpha (PGF2alpha) to sexually mature female pigs induces luteolysis and rapidly elicits a behavioural response consistent with pre-partum nest-building. Intramuscular injection of the synthetic prostaglandin F2alpha (cloprostenol) also induces luteolysis but no nest-building behaviour is observed. The effects of PGF2alpha, but not cloprostenol, on nest-building behaviour may be mediated via peripheral PGF2alpha receptors (FP) or via direct action on central FP receptors. We have previously shown FP receptor mRNA to be localized in porcine paraventricular nucleus (PVN), supraoptic nucleus (SON) and pars dorso-medialis of the suproptic nucleus (SOD), suprachiasmatic nucleus, choroid plexus and anterior and intermediate pituitary lobes. In this experiment, we examined hypothalamic expression of the immediate early genes c-fos and c-jun mRNA after treatment with PGF2alpha or cloprostenol. Twenty-one 8-month-old nulliparous female pigs (gilts) were injected intramuscularly with a luteolytic dose of PGF2alpha (15 mg), cloprostenol (175 microg) or saline control, their behaviour was recorded and they were killed 60 min later. Coronal hypothalamic sections and control ovarian tissues were incubated with 45-mer oligonucleotide probes complementary to porcine c-fos and c-jun genes using standard in situ hybridization histochemistry techniques. Significantly higher c-fos and c-jun mRNA expression was found in PGF2alpha-treated compared to saline or cloprostenol-treated pigs in the PVN, SON and SOD. Significantly higher c-fos and c-jun mRNA expression was found in corpus lutea of PGF2alphaand cloprostenol-treated pigs compared to saline controls. Treatment with PGF2alpha induced nest-building behaviour whereas treatment with cloprostenol and saline did not. This suggests that PGF2alpha, or one of its metabolites, and not cloprostenol, crosses the blood-brain barrier and acts directly on hypothalamic receptors to mediate its effect on nest-building behaviour.
Walton
S L
SL
Department of Neurobiology, The Babraham Institute, Babraham, Cambridge, UK. sarah.walton@bbsrc.ac.uk
Burne
T H J
TH
Gilbert
C L
CL
D000818:Animals; D003008:Cloprostenol / Q000737:chemistry / Q000494:pharmacology; D015237:Dinoprost / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D009425:Nesting Behavior / Q000187:drug effects*; D010120:Oxytocics / Q000737:chemistry / Q000494:pharmacology*; D010286:Paraventricular Hypothalamic Nucleus / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D013495:Supraoptic Nucleus / Q000502:physiology*; D013552:Swine
D010120:Oxytocics; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D003008:Cloprostenol; D015237:Dinoprost