title: Conformational studies of wheat flour high relative molecular mass glutenin subunits by circular dichroism spectroscopy.
abstract: Conformational studies of 1Dx2, 1Bx7, and 1Dy12 high relative molecular mass glutenin subunits, extracted from Alisei 1 flour, are reported. Circular dichroism (CD) spectroscopy is employed to study their conformational polymorphism induced by urea and by urea in the presence of 1% sodium dodecyl sulfate (SDS). The CD spectra indicate that SDS promotes ordered structures. The addition of urea to the SDS-acetate solution of 1Dx2, 1Bx7, and 1Dy12 subunits eliminates the effect of SDS. Its addition to the acetate solution of proteins induces conformational transitions to form a poly-L-proline II-like structure. All the changes induced by urea follow a multistep transition process that is typical of proteins consisting of different domains.
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Catania, V. le A. Doria 6, 95125 Catania, Italy. sfisichella@dipchi.unict.it
authors.forename: S
authors.initials: S
authors.lastname: Fisichella
authors.forename: G
authors.initials: G
authors.lastname: Alberghina
authors.forename: M E
authors.initials: ME
authors.lastname: Amato
authors.forename: M
authors.initials: M
authors.lastname: Fichera
authors.forename: D
authors.initials: D
authors.lastname: Mantarro
authors.forename: A
authors.initials: A
authors.lastname: Palermo
authors.forename: A
authors.initials: A
authors.lastname: Savarino
authors.forename: G
authors.initials: G
authors.lastname: Scarlata
mesh_terms: D002942:Circular Dichroism; D005983:Glutens / Q000031:analogs & derivatives* / Q000737:chemistry*; D008970:Molecular Weight; D011487:Protein Conformation / Q000187:drug effects; D021122:Protein Subunits; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D012996:Solutions; D014908:Triticum / Q000737:chemistry*; D014508:Urea / Q000494:pharmacology
chemical_list: D021122:Protein Subunits; D012996:Solutions; D012967:Sodium Dodecyl Sulfate; D005983:Glutens; D014508:Urea; C037302:glutenin