[Effects of serum-free murine bone marrow endothelial cell conditioned medium on the growth of CFU-E and BFU-E].
Serum-free murine bone marrow endothelial cell conditioned medium(mBMEC-cm) was collected. The mBMEC-cm was ultrafiltered in Centriprep. The concentrated retentive substance of mBMEC-cm (MW > 10,000) and filtrate of mBMEC-cm(MW < 10,000) were obtained. The effects of mBMEC-cm on the growth of CFU-E and BFU-E were investigated. The results showed: 1. The retentive substance of mBMEC-cm significantly enhanced the growth of CFU-E and BFU-E. The effects of mBMEC-cm(2%-6%, V/V) on the growth of CFU-E and BFU-E were dose-dependent. 2. The filtrate of mBMEC-cm markedly inhibited the growth of CFU-E and BFU-E. The effects of the filtrate of mBMEC-cm on the growth of CFU-E and BFU-E were also dose-dependent. The mechanism of regulation will be studied further.
Huang
Y H
YH
Research Laboratory of Blood Physiology, Hunan Medical University, Changsha 410078.
Li
W M
WM
Jiang
D Z
DZ
Wang
Q R
QR
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002455:Cell Division; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016895:Culture Media, Serum-Free; D004727:Endothelium / Q000166:cytology*; D015672:Erythroid Precursor Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C
D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free