title: Maltooligosaccharide disproportionation reaction: an intrinsic property of amylosucrase from Neisseria polysaccharea.
abstract: Amylosucrase from Neisseria polysaccharea (AS) is a remarkable transglycosidase of family 13 of the glycoside hydrolases that catalyses the synthesis of an amylose-like polymer from sucrose and is always described as a sucrose-specific enzyme. Here, we demonstrate for the first time the ability of pure AS to catalyse the disproportionation of maltooligosaccharides by cleaving the alpha-1,4 linkage at the non-reducing end of a maltooligosaccharide donor and transferring the glucosyl unit to the non-reducing end of another maltooligosaccharide acceptor. Surprisingly, maltose, maltotriose and maltotetraose are very poor glucosyl donors whereas longer maltooligosaccharides are even more efficient glucosyl donors than sucrose. At least five glucose units are required for efficient transglucosylation, suggesting the existence of strong binding subsites, far from the sucrose binding site, at position +4 and above.
authors.affiliation: Centre de Bioingénierie Gilbert Durand, UMR CNRS 5504, UMR INRA 792, INSA, 135 avenue de Rangueil, 31077 Cedex 4, Toulouse, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Albenne
authors.forename: Lars K
authors.initials: LK
authors.lastname: Skov
authors.forename: Osman
authors.initials: O
authors.lastname: Mirza
authors.forename: Michael
authors.initials: M
authors.lastname: Gajhede
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Potocki-Véronèse
authors.forename: Pierre
authors.initials: P
authors.lastname: Monsan
authors.forename: Magali
authors.initials: M
authors.lastname: Remaud-Simeon
mesh_terms: D002236:Carbohydrate Conformation; D005964:Glucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D007700:Kinetics; D008320:Maltose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D009343:Neisseria / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D013395:Sucrose / Q000737:chemistry; D014312:Trisaccharides / Q000737:chemistry / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D014312:Trisaccharides; C021705:maltooligosaccharides; D013395:Sucrose; C008317:maltotriose; D008320:Maltose; C009819:maltotetraose; D005964:Glucosyltransferases; C019700:amylosucrase