title: A novel gene, NMES1, downregulated in human esophageal squamous cell carcinoma.
abstract: To isolate genes with different expression levels in human esophageal SCC, SSH and reverse Northern were performed between cancer tissue and its normal counterpart. Among the differentially expressed genes identified, we report here cDNA corresponding to a 0.88 kb mRNA (NMES1), whose expression was observed in all 36 adjacent normal esophageal mucosae, while 31 (86%) matched cancer tissues showed a marked reduction or complete lack of its expression. Sequence analysis of its full-length cDNA revealed a gene encoding a predicted polypeptide of 9 kDa. Northern blot showed that NMES1 was distributed mainly in the alimentary tract. The gene was mapped to 15q21.1 by screening the GenBridge 4 RH panel. Immunohistochemistry confirmed the downregulation of NMES1 in esophageal SCC at the protein level and showed that it is a nuclear protein. In situ hybridization revealed its different expression during mouse embryonic development, especially in bone, brain, stomach and intestine. The negative correlation of NMES1 expression with esophageal oncogenesis suggests its suppressive role in tumorigenesis of the esophagus, while the precise function of NMES1 still needs further investigation.
authors.affiliation: National Laboratory of Molecular Oncology, Department of Cell Biology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Huixin
authors.initials: H
authors.lastname: Wang
authors.forename: Ailing
authors.initials: A
authors.lastname: Lu
authors.forename: Gengxi
authors.initials: G
authors.lastname: Hu
authors.forename: Aiping
authors.initials: A
authors.lastname: Luo
authors.forename: Fang
authors.initials: F
authors.lastname: Ding
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D018076:DNA, Complementary / Q000302:isolation & purification; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D009687:Nuclear Proteins; D009693:Nucleic Acid Hybridization; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; C465665:C15orf48 protein, human