New prevalence estimate of TT virus (TTV) infection in low- and high-risk population from São Paulo, Brazil.
The prevalence of TT virus (TTV) infection was investigated by Polymerase Chain Reaction (PCR) in low- (blood donors and healthy children/adolescents) and high-risk (hemophiliacs) groups from São Paulo, Brazil. Primers based on the untranslated region (UTR) of the viral genome proved to be much more ubiquitous, leading to much higher frequencies for both groups (>or= 81%) than the earlier N22-PCR directed to the open reading frame 1 (blood donors, 5.5%, and hemophiliacs, 42.3%). The UTR-PCR also revealed an interesting profile for healthy children/adolescents: very high prevalence at the early years and significant decrease in male teenagers. The N22-PCR, in turn, demonstrated higher frequency in hemophiliacs treated with fresh blood products (58%), than in those treated with virus-inactivated clotting factors (9.4%) and blood donors (5.5%).
Bassit
Leda
L
Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, SP, Brazil.
Takei
Kioko
K
Hoshino-Shimizu
Sumie
S
Nishiya
Anna S
AS
Sabino
Ester C
EC
Bassitt
Rogério P
RP
Focaccia
Roberto
R
D'Amico
Elbio
E
Chamone
Dalton F
DF
Ribeiro-Dos-Santos
Gabriela
G
D000293:Adolescent; D001782:Blood Donors; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004266:DNA Virus Infections / Q000175:diagnosis / Q000453:epidemiology*; D005260:Female; D006467:Hemophilia A / Q000821:virology; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D016036:Seroepidemiologic Studies; D022783:Torque teno virus; D020506:Untranslated Regions
D020506:Untranslated Regions