The Recombination and Expression of a Chimeric Antibody Light Chain Gene in Cells of Silkworm Bombyx mori.
A chimeric immunoglobulin light chain gene had been expressed in a baculovirus expression system, which was achieved by infecting the silkworm cells with the recombinant virus r-NPVL2 containing the cDNA of immunoglobulin against a small cell lung cancer. PCR analysis and Southern hybridization proved that the antibody light chain cDNA hand been integrated into the modified BmNPV genome. Expression products of human-mouse chimeric antibody light chain in the silkworm cells were detected by both Western blot and ELISA.
Yang
Guan-Zhen
GZ
Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
Wu
Xiang-Fu
XF
Zhang
Wen-Xiang
WX
Li
Si-De
SD