Linear dichroism for the detection of single base pair mutations.
Flow linear dichroism is shown to be able to detect single base mismatches in a polymerase chain reaction (PCR) amplimers from exon 10 of the human beta-glucocerebrosidase gene (associated with Gaucher disease) over a kilobase long with no post PCR manipulation.
Halsall
D J
DJ
Department of Clinical Biochemistry, Addenbrooke's Hospital, Hills Rd, Cambridge, UK CB2 2XY.
Rodger
A
A
Dafforn
T R
TR
D000483:Alleles; D020137:Base Pair Mismatch; D005091:Exons / Q000235:genetics; D005776:Gaucher Disease / Q000235:genetics*; D005962:Glucosylceramidase / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D013057:Spectrum Analysis
D005962:Glucosylceramidase