title: Nitric oxide synthase in the frog cerebellum: response of Purkinje neurons to unilateral eighth nerve transection.
abstract: When vestibular damage occurs, nitric oxide synthase (NOS) expression in rat cerebellar flocculus is affected. Since compensation for postural symptoms occurs and Purkinje cells play an important role in movement coordination and motor learning, we analyzed in situ the induction of NOS in the Purkinje cell population of the cerebellum (corpus cerebelli) of frog after unilateral transection of the eighth statoacoustic nerve to gain insight into the role of NO in neural plasticity after injury. Three days after neurectomy, the early effects induced NADPH diaphorase reactivity in most of the Purkinje cells on the ipsilateral side, while on the contralateral side the highest labeling was observed at 15 days. This finding can give information on the dynamics of vestibular compensation, in which NOS involvement was investigated. At 30 days, NADPH diaphorase reactivity was present in a large number of Purkinje cells of the whole cerebellum, while at 60 days a down-regulation for NADPH diaphorase reactivity was evident. A similar trend was observed for NOS-immunoreactivity, which was still present at 60 days in a high percentage of Purkinje cells, mainly on the ipsilateral side. On the basis of cell density evaluations, it was proposed that the early induction of NOS after neurectomy was linked to the degeneration of a part of the Purkinje neurons, while the permanence of NOS labeling might be due to a neuroprotective role of NO in the restoration phase of the vestibular compensation process.
authors.affiliation: Dipartimento di Biologia Animale, Università di Pavia, Pavia, Italy.
authors.forename: Maria Bonaria
authors.initials: MB
authors.lastname: Pisu
authors.forename: Elena
authors.initials: E
authors.lastname: Conforti
authors.forename: Laura
authors.initials: L
authors.lastname: Botta
authors.forename: Paolo
authors.initials: P
authors.lastname: Valli
authors.forename: Graziella
authors.initials: G
authors.lastname: Bernocchi
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D016923:Cell Death / Q000502:physiology; D015536:Down-Regulation / Q000502:physiology; D007839:Functional Laterality / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D023421:Models, Animal; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D011187:Posture / Q000502:physiology; D011689:Purkinje Cells / Q000166:cytology / Q000201:enzymology*; D011893:Rana esculenta / Q000033:anatomy & histology / Q000378:metabolism*; D011930:Reaction Time / Q000502:physiology; D020127:Recovery of Function / Q000502:physiology; D015854:Up-Regulation / Q000502:physiology; D014725:Vestibular Nerve / Q000293:injuries* / Q000503:physiopathology / Q000601:surgery; D000160:Vestibulocochlear Nerve Diseases / Q000201:enzymology* / Q000503:physiopathology
chemical_list: D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase