title: Isolation of ovocleidin-116 from chicken eggshells, correction of its amino acid sequence and identification of disulfide bonds and glycosylated Asn.
abstract: Fractionation of the soluble chicken eggshell matrix by chromatographic methods yielded 13 endogenous proteolytic fragments of the eggshell-specific proteoglycan core protein ovocleidin-116. The N-terminal amino acid sequences of these fragments in general confirmed the recently cDNA-deduced sequence of ovocleidin-116, with one exception. One fragment yielded a completely new sequence and was instrumental in detecting a frame shift error in the nucleotide sequence. The correction yielded a new sequence which was 38 amino acids shorter than before and contained a 57-amino acid long novel C-terminal sequence. The predicted sequence of ovocleidin-116 contained two consensus N-glycosylation sites, only one of which (Asn62) was found to be fully modified. A disulfide bond was identified between Cys31 and 42 implying that Cys329 and 421 form a second disulfide bond. Finally, the yield of fragments indicated that ovocleidin-116 is a major component of the chicken eggshell matrix.
authors.affiliation: Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, D-82152 Martinsried, Germany. mann@biochem.mpg.de
authors.forename: Karlheinz
authors.initials: K
authors.lastname: Mann
authors.forename: Maxwell T
authors.initials: MT
authors.lastname: Hincke
authors.forename: Yves
authors.initials: Y
authors.lastname: Nys
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001216:Asparagine; D001483:Base Sequence / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D002645:Chickens; D004220:Disulfides / Q000737:chemistry; D004527:Egg Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D004528:Egg Shell / Q000737:chemistry*; D006031:Glycosylation; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry
chemical_list: D004220:Disulfides; D004527:Egg Proteins; D010446:Peptide Fragments; C402224:ovocleidin-116; D001216:Asparagine