title: Integrated classification of lung tumors and cell lines by expression profiling.
abstract: The utility of cancer cell lines depends largely on their accurate classification, commonly based on histopathological diagnosis of the cancers from which they were derived. However, because cancer is often heterogeneous, the cell line, which also has the opportunity to alter in vitro, may not be representative. Yet without the overall architecture used in histopathological diagnosis of fresh samples, reclassification of cell lines has been difficult. Gene-expression profiling accurately reproduces histopathological classification and is readily applicable to cell lines. Here, we compare the gene-expression profiles of 41 cell lines with 44 tumors from lung cancer. These profiles were generated after hybridization of samples to four replicate 7,685-element cDNA microarrays. After removal of genes that were uniformly up- or down-regulated in fresh compared with cell-line samples, cluster analysis produced four major branch groups. Within these major branches, fresh tumor samples essentially clustered according to pathological type, and further subclusters were seen for both adenocarcinoma (AC) and small cell lung carcinoma (SCLC). Four of eight squamous cell carcinoma (SCC) cell lines clustered with fresh SCC, and 11 of 13 SCLC cell lines grouped with fresh SCLC. In contrast, although none of the 11 AC cell lines clustered with AC tumors, three clustered with SCC tumors and six with SCLC tumors. Although it is possible that preexisting SCC or SCLC cells are being selected from AC tumors after establishment of cell lines, we propose that, even in situ, AC will ultimately progress toward one of two poorly differentiated phenotypes with expression profiles resembling SCC or SCLC.
authors.affiliation: Chugai Pharmaceuticals, 3-41-8 Takada, Toshima-ku, Tokyo 171-8545, Japan.
authors.forename: Carl
authors.initials: C
authors.lastname: Virtanen
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Honjoh
authors.forename: Mami
authors.initials: M
authors.lastname: Kimura
authors.forename: Miyuki
authors.initials: M
authors.lastname: Shimane
authors.forename: Tatsu
authors.initials: T
authors.lastname: Miyoshi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nomura
authors.forename: Michael H
authors.initials: MH
authors.lastname: Jones
mesh_terms: D000230:Adenocarcinoma / Q000145:classification / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics; D018287:Carcinoma, Large Cell / Q000145:classification / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000145:classification / Q000235:genetics; D018288:Carcinoma, Small Cell / Q000145:classification / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000145:classification / Q000235:genetics; D002454:Cell Differentiation; D016000:Cluster Analysis; D004273:DNA, Neoplasm / Q000235:genetics; D020869:Gene Expression Profiling / Q000706:statistics & numerical data; D006801:Humans; D008175:Lung Neoplasms / Q000145:classification* / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000706:statistics & numerical data; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm