[Studies on quality standards for Pollen Typhae(puhuang)].
OBJECTIVE
To work out quality standards for Pollen Typhae(Puhuang).


METHODS
Isorhamnetin-3-O-neohesperidoside, typhaneoside, isorhamnetin and beta-sitosterol in Pollen Typhae marketed in ten cities of China were identified by TLC. The contents of isorhamnetin-3-O-neohesperidoside and typhaneoside were determined by HPLC.


RESULTS
The method is sensitive and specific for identifying isorhamnetin-3-O-neohesperidoside, typhaneoside, isorhamnetin and beta-sitosterol. The linear ranges of isorhamnetin-3-O-neohesperidoside and typhaneoside were 0.188-0.940 microgram with and average recovery of 97.77%; and 0.164-0.820 microgram with an average recovery of 98.42% respectively.


CONCLUSIONS
The method can be used both qualitatively and quantitatively as standards for the quality control of Pollen Typhae.
Zhang
S H
SH
Hunan Academy of Traditional Chinese Medicine and Materia Medica, Changsha 410006, Hunan, China.
Wang
S Q
SQ
Liu
C H
CH
Yang
Y H
YH
D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000592:standards*; D044948:Flavonols; D006027:Glycosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry; D011786:Quality Control; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis*; D032401:Typhaceae / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D044948:Flavonols; D006027:Glycosides; C047368:3-methylquercetin; C051428:typhaneoside; D011794:Quercetin