[Cloning and analysis of different regions of SRY promoter].
The luciferase systems were used to assay the promoter activity of SRY gene with clones of different parts of the 5' flanking region within 544 bp which has basal promoter activity. The results were that the 179 bp region from nt. -353 to nt. -174 upstream of the first ATG included a silent element; the 49 bp region from nt. -112 to nt. -63 included an enhanced element and the 63 bp region from nt. -174 to nt. -112 included an essential promoter sequency for gene transcription. These results give some important clues to elucidate the expression and regulation mechanisms of SRY gene.
Yuan
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C
Research Centre of Molecular Biology, Hunan Medical University, Changsha 410078.
Zhu
M
M
Liu
Z
Z
Xie
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SS
D024506:5' Flanking Region / Q000235:genetics*; D003001:Cloning, Molecular; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005786:Gene Expression Regulation; D024282:Genes, sry / Q000235:genetics*; D006367:HeLa Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008156:Luciferases; D008297:Male; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014162:Transfection
D008156:Luciferases