Group IB secretory phospholipase A(2)induces cell death in the cultured cortical neurons: a possible involvement of its binding sites.
In primary cultures of rat cortical neurons, group IB secretory phospholipase A(2) (sPLA(2)-IB) induced cell death. In rat cortical membranes, there were high affinity binding sites of [125I]sPLA(2)-IB. The high-affinity binding sites were decreased by sPLA(2)-IB and anti-sPLA(2) receptor immunoglobulin G (anti-sPLA(2)R IgG). Furthermore, anti-sPLA(2)R IgG caused neuronal cell death in a concentration-dependent manner. The present study suggests that sPLA(2)-IB induces neuronal cell death via its high-affinity binding sites.
Yagami
Tatsurou
T
Discovery Research Laboratories, Shionogi and Co Ltd, 12-4 Sagisu 5-Chome, Fukushima-ku, Osaka 553-0002, Japan. tatsurou.yagami@shionogi.co.jp
Ueda
Keiichi
K
Asakura
Kenji
K
Hata
Satoshi
S
Kuroda
Takayuki
T
Sakaeda
Toshiyuki
T
Kishino
Jyunji
J
Sakaguchi
G
G
Itoh
Naohiro
N
Hori
Yozo
Y
D000818:Animals; D001665:Binding Sites; D018929:Cell Culture Techniques; D016923:Cell Death / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D010741:Phospholipases A / Q000009:adverse effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D010741:Phospholipases A