title: Enumeration, phenotyping, and identification of activation events in conjugates between T cells and antigen-presenting cells by flow cytometry.
abstract: Microscopic analysis of T cell-antigen-presenting cell (T cell:APC) interactions at the single cell level has been a powerful, but tedious and subjective, technique. In this paper, we describe a rapid and quantitative method to identify T cell:APC conjugates using succinimidyl ester dyes, which irreversibly label free amine groups on the cell surface. The labeled cell conjugates and subsequent activation events are detected by flow cytometry.
authors.affiliation: Integrated Department of Immunology, National Jewish Medical and Research Center and the University of Colorado Health Sciences Center, 1400 Jackson Street, Denver, CO 80206, USA.
authors.forename: Kristie M
authors.initials: KM
authors.lastname: Grebe
authors.forename: Terry A
authors.initials: TA
authors.lastname: Potter
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism*; D016827:CD8 Antigens / Q000032:analysis; D002450:Cell Communication / Q000276:immunology*; D002452:Cell Count; D002460:Cell Line; D002463:Cell Membrane Permeability; D005434:Flow Cytometry / Q000379:methods; D006834:Hydrazines / Q000032:analysis; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D051379:Mice; D008822:Mice, Transgenic; D019000:Phosphotyrosine / Q000032:analysis / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*
chemical_list: C417561:Alexa 488 hydrazide; D016827:CD8 Antigens; D006834:Hydrazines; D019000:Phosphotyrosine