title: Assessment of human exposure to PCDDs, PCDFs and Co-PCBs using hair as a human pollution indicator sample I: Development of analytical method for human hair and evaluation for exposure assessment.
abstract: Dioxins including polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and co-planar polychlorinated biphenyls (Co-PCBs) are highly toxic. Even at environmental pollution levels, they cause hormonal damage in women, and they have been shown to induce immunosuppression and genital function damage in humans. In this study, a new method using isotope dilution was established to detect PCDDs, PCDFs and Co-PCBs in human hair. This method, comprised of washing and cutting of hair, alkaline decomposition, hexane extraction, multilayer silica gel column chromatography, high performance liquid chromatography with a porous graphite carbon column and analysis by high resolution gas chromatography/high resolution mass spectrometry, enabled us to analyze PCDDs, PCDFs and Co-PCBs at trace levels of less than pg/g with good reproducibility. In addition, there was a correlation between some isomers in human hair and blood collected from identical donors. Human hair analysis is useful to evaluate human risk assessment including that due to environmental pollution.
authors.affiliation: Faculty of Pharmaceutical Science, Setsunan University, Hirakata, Osaka, Japan. nakao@pharm.setsunan.ac.jp
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nakao
authors.forename: Osamu
authors.initials: O
authors.lastname: Aozasa
authors.forename: Souichi
authors.initials: S
authors.lastname: Ohta
authors.forename: Hideaki
authors.initials: H
authors.lastname: Miyata
mesh_terms: D001572:Benzofurans / Q000032:analysis* / Q000493:pharmacokinetics; D000072338:Dibenzofurans, Polychlorinated; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis* / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry; D006801:Humans; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000493:pharmacokinetics; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis* / Q000493:pharmacokinetics
chemical_list: D001572:Benzofurans; D000072338:Dibenzofurans, Polychlorinated; D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D012989:Soil Pollutants; D011078:Polychlorinated Biphenyls