title: A method to detect major serotypes of foot-and-mouth disease virus.
abstract: Nucleic acid sequence-based amplification (NASBA) is an isothermal technique that allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A rapid and specific NASBA technique was developed, allowing the detection of foot-and-mouth disease virus genetic material in a range of sample material, including preserved skin biopsy material from infected animals, vaccines prepared from denatured cell-free material, and cell-free antigen-based detection kits. A single pair of DNA oligonucleotide primers was able to amplify examples of all major FMD virus subtypes. The amplified viral RNA was detected by electrochemiluminescence. The method was at least as sensitive as existing cell-free antigen detection methods.
authors.affiliation: Hong Kong DNA Chips Ltd, 1805-6, 18/F, Lu Plaza, Kowloon, Hong Kong SAR, China.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Collins
authors.forename: Lung-Sang
authors.initials: LS
authors.lastname: Ko
authors.forename: King Yip
authors.initials: KY
authors.lastname: Fung
authors.forename: Lok-Ting
authors.initials: LT
authors.lastname: Lau
authors.forename: Jun
authors.initials: J
authors.lastname: Xing
authors.forename: Albert C H
authors.initials: AC
authors.lastname: Yu
mesh_terms: D000818:Animals; D002417:Cattle; D017931:DNA Primers / Q000235:genetics / Q000378:metabolism; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000276:immunology; D008163:Luminescent Measurements; D011189:Potassium Chloride / Q000378:metabolism; D021161:Self-Sustained Sequence Replication / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping
chemical_list: D017931:DNA Primers; D011189:Potassium Chloride