title: The Application of Monoclonal Antibodies to the Imunoaffinity Purification of Human Ferritin.
abstract: There was difference between the reactivities of anti-human ferritin monoclonal antibodies, 6D6 and A-hF-C with liver and heart ferritins. 6D6 reacts with liver and heart ferritin with similar intensity, while A-hF-C reacts preferentially with liver ferritin. Two affinity gels were made with 6D6 and A-hF-C respectively, and used to purify ferritins from crude extracts of human liver and heart ferritins. The results have showed that it is a simple and time-saving method for the purification of ferritin with high purity and yield.
authors.affiliation: Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
authors.forename: De-Sheng
authors.initials: DS
authors.lastname: Zhang
authors.forename: Hui-Ting
authors.initials: HT
authors.lastname: Cao
authors.forename: Ching-I
authors.initials: CI
authors.lastname: Niu
authors.forename: Zu-Chuan
authors.initials: ZC
authors.lastname: Zhang