-11 Mutation in the ampC promoter increasing resistance to beta-lactams in a clinical Escherichia coli strain.
A mutation was discovered in the Pribnow box of the ampC promoter in a clinical Escherichia coli strain. This -11 C-to-T transition created a perfect homology with the -10 consensus sequence. The new promoter was cloned upstream of the cat gene of pKK232-8 and induced a sixfold increase in promoter strength.
Corvec
S
S
Laboratoire de Bactériologie-Virologie, Hygiène hospitalière, CHU, Université de Nantes, France. stephane.corvec@chu-nantes.fr
Caroff
N
N
Espaze
E
E
Marraillac
J
J
Reynaud
A
A
D001426:Bacterial Proteins; D001483:Base Sequence; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D011401:Promoter Regions, Genetic; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
D001426:Bacterial Proteins; D047090:beta-Lactams; C048795:AmpC beta-lactamases; D001618:beta-Lactamases