title: The disaccharide anthracycline MEN 10755 binds human serum albumin to a non-classical drug binding site.
abstract: The interaction of the novel disaccharide anthracycline MEN 10755 with human serum albumin (HSA) was investigated by visible absorption and fluorescence spectroscopies and by ultrafiltration. Notably, MEN 10755 binds serum albumin far stronger than doxorubicin. Albumin binding results into a drastic quenching of the intrinsic fluorescence of MEN 10755; a binding constant of 1.1 x 10(5) was determined from fluorescence data. To localize the HSA binding site of MEN 10755 competition experiments were carried out with ligands that are selective for the different drug binding sites of the protein. No relevant competition effects were seen in the case of warfarin, diazepam and hemin, known ligands of sites I, II and III, respectively. Modest effects were observed following addition of palmitic acid that targets the several fatty acid binding sites of the protein. In contrast, extensive displacement of the bound anthracycline was achieved upon addition of ethacrinic acid. On the basis of these results, it is proposed that MEN 10755 binds serum albumin tightly to a non-canonical surface binding site for which it competes specifically with ethacrinic acid.
authors.affiliation: Department of Chemistry, University of Florence, Via Gino Capponi 7, Florence, Italy. messori@cerm.unifi.it
authors.forename: Luigi
authors.initials: L
authors.lastname: Messori
authors.forename: Francesca
authors.initials: F
authors.lastname: Piccioli
authors.forename: Silvia
authors.initials: S
authors.lastname: Gabrielli
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Orioli
authors.forename: Leonardo
authors.initials: L
authors.lastname: Angeloni
authors.forename: Cristina
authors.initials: C
authors.lastname: Di Bugno
mesh_terms: D000465:Algorithms; D000970:Antineoplastic Agents / Q000378:metabolism*; D001665:Binding Sites; D004187:Disaccharides / Q000378:metabolism*; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014462:Ultrafiltration
chemical_list: D000970:Antineoplastic Agents; D004187:Disaccharides; D008024:Ligands; D012709:Serum Albumin; D004317:Doxorubicin; C107602:sabarubicin