Expression of Nogo protein by growing axons in the developing nervous system.
We produced monoclonal antibody NG1 that strongly binds growing axons in the developing nervous system of mice. This antibody intensely labeled the growth cone of cultured neurons. Although these immunostaining patterns suggested the association of growing axons with the antigen recognized by this antibody, the antigen was identified as Nogo protein, an axonal repulsive factor isolated from the myelin. On the basis of this unexpected finding, we discuss the possible functions of Nogo in the developing nervous system.
Tozaki
Hirokazu
H
Division of Brain Function, National Institute of Genetics, Graduate University for Advanced Studies, Yata 1111, Mishima, Japan.
Kawasaki
Takahiko
T
Takagi
Yoshiko
Y
Hirata
Tatsumi
T
D000818:Animals; D002450:Cell Communication / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D002633:Chemotaxis / Q000502:physiology; D006224:Cricetinae; D005260:Female; D020439:Growth Cones / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D009185:Myelin Proteins / Q000276:immunology / Q000378:metabolism*; D000070798:Nogo Proteins; D009830:Olfactory Bulb / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D009833:Olfactory Pathways / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding / Q000276:immunology; D017434:Protein Structure, Tertiary / Q000235:genetics
D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603201:Rtn4 protein, mouse