title: Purification and Characterization of Porin from Corn (Zea mays L.) Mitochondria.
abstract: Mitochondrial porin from corn (Zea mays L. B 73) shoots was solubilized with lauryl(dimethyl)-amine oxide and purified by chromatography on a hydroxyapatite:celite column. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified protein had an apparent molecular mass of 35 kD. When reconstituted in planar lipid bilayer membranes the porin formed ion-permeable channels with single-channel conductance of 2.0 and 4.0 nanosiemens in 1 M KCl. At low transmembrane voltages corn porin had the properties of a general diffusion pore with an estimated effective diameter of 1.6 nm and a small selectivity for anions over cations. The primary structure of corn porin seems to be quite different from that of other mitochondrial porins, because it did not cross-react with monoclonal antibodies against human porin and with polyclonal antibodies against yeast porin. Furthermore, the peptide maps of corn and bovine heart porins were very different. A sequence of 21 amino acids obtained by Edman degradation of peptides generated by porin proteolysis with Staphylococcus aureus V8 protease did not show any significant homology with known sequences of mitochondrial porins. Results of our investigation suggest that corn porin possesses functional properties similar to those of other mitochondrial porins, despite major structural differences.
authors.affiliation: Department of Pharmaco-Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari and Consiglio Nazionale delle Ricerche Unit for the Study of Mitochondria, Bari, Italy (J.A.A., G.G., V.D.P., L.S., F.P).
authors.forename: J. A.
authors.initials: JA
authors.lastname: Aljamal
authors.forename: G.
authors.initials: G
authors.lastname: Genchi
authors.forename: V.
authors.initials: V
authors.lastname: De Pinto
authors.forename: L.
authors.initials: L
authors.lastname: Stefanizzi
authors.forename: A.
authors.initials: A
authors.lastname: De Santis
authors.forename: R.
authors.initials: R
authors.lastname: Benz
authors.forename: F.
authors.initials: F
authors.lastname: Palmieri