[Effect of in vitro interferon on bone marrow megakaryocyte in patients with idiopathic thrombocytopenic purpura].
The effect of rIFN alpha-2a on the megakaryocyte colony growth and maturation was observed in 33 patients with idiopathic thrombocytopenic purpura (CITP) in vitro by plasma clot cultures and by GP III a McAb and ABC kit. Twenty five healthy persons were in the control group. The results showed that the number of colony formation units of megakaryocyte in the patients was similar to that in the controls, but the diameter and area levels of megakaryocyte in the patients were lower. The ratio of inhibition of rIFN alpha-2a on megakaryocyte progenitors colony growth was significantly lower in the patients and was lowest in the group with increasing number of megakaryocytes on bone marrow smear. The rIFN alpha-2a had no effect on promoting the megakaryocyte maturation. Since this study has indicated that the inhibition of rIFN alpha-2a on colony formation unit of megakaryocyte is slight in CITP, interferon therapy should be a suitable prescription for the CITP patients who have an increasing number of megakaryocyte on bone marrow smear.
Xue
M
M
Department of Hematology, First Affiliated Hospital, WCUMS, Chengdu 610041.
Deng
C
C
Meng
W
W
Wei
J
J
D000293:Adolescent; D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D008297:Male; D008533:Megakaryocytes / Q000166:cytology*; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood*; D011994:Recombinant Proteins
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins