[Expression of ET-1 and Pr ET-1 mRNA in kidney of early diabetic rats].
The purpose of this study was to explore the role of endothelin 1 (ET-1) and pr ET-1 mRNA in kidney of early diabetic rats. The location and level of ET-1 were determined by immuno-histochemical staining; ET-1 cDNA probe was used for in situ hybridization. The expressions of ET-1 and ET-1 mENA in the kidneys of 24 early diabetic rats were detected by microscopy and Mia-2000 patho-image-analysis system. The results revealed that the ET-1 mRNA and ET-1 were principally distributed in the glomerular endothelial cells, mesangial cells, smooth muscle cells of vessels, epithelial cell lining tubules and collecting ducts, showing no difference in the distribution over kidney, but the expressions of ET-1 and ET-1 mRNA were higher in the renal medulla. The levels of ET-1 and ET-1 mRNA in glomeruli significantly decreased at week 1 and week 2(P < 0.05), but increased at week 4(P < 0.05). However, the levels of ET-1 and ET-1 mRNA continually increased from week 1 to week 4 in the medulla of diabetic rats. These data suggest that ET-1 and ET-1 mRNA may induce the glomerular hyperperfusion and damage the tubulointerstitial of the kidneys in early diabetic rats.
Huang
S
S
Department of Nephropathy, First Affiliated Hospital, WCUMS, Chengdu 610041.
Chen
M
M
Li
Y
Y
Tian
Q
Q
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000378:metabolism*; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000235:genetics; D007668:Kidney / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D019332:Endothelin-1; D012333:RNA, Messenger