Identification of Trichophyton rubrum by PCR  fingerprinting.
OBJECTIVE
To observe the unique DNA profile and the relationship between DNA profile and phenotype of Trichophyton rubrum,and establish an effective molecular method to identify T. rubrum.


METHODS
Three primers, including (GACA)(4), (GTG)(5) and M13 core sequence (5'-GAGGGTG-GCGGTTCT-3'), were used to distinguish variations among 20 clinical isolates of T. rubrum and Trichophyton mentagrophytes.


RESULTS
Different PCR-fingerprinting was seen between T. rubrum and T. mentagrophytes using three different primers. 2 stains of T. rubrum were identified among 6 supposed T. mentagrophytes.


CONCLUSIONS
T. rubrum can be distinguished using PCR, and (GACA)(4) is the most suitable primer.
Zhu
Hongmei
H
Cyptoccucus Laboratory, Department of Dermatology, Changzheng Hospital Second Military Medical University, Shanghai 200003, China. hmzhu__cn@yahoo.com.cn
Wen
Hai
H
Liao
Wanqing
W
D016172:DNA Fingerprinting / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D014249:Trichophyton / Q000235:genetics / Q000302:isolation & purification*