Monitoring cytomegalovirus infection by antigenemia assay and two distinct plasma real-time PCR methods after hematopoietic stem cell transplantation.
We compared a CMV virus load determined by real-time PCR with an antigenemia value to analyze the correlation between these two methods. We also compared the values for virus load determined by the two distinct real-time PCR methods, which amplify the US17 region and immediate-early (IE) gene of CMV, respectively, to evaluate the reliability of these methods. Two hundred and sixty-five samples were obtained weekly from 29 patients, who had engraftment after unrelated bone marrow transplantation or HLA-mismatched related blood stem cell transplantation. CMV infection was detected in 115 samples from 22 patients by US17-PCR and 69 samples from 20 patients by the antigenemia assay. Fifty-eight samples were positive for both assays, but 57 and 11 samples were positive only for US17-PCR and antigenemia, respectively. A good correlation of the results of US17-PCR and antigenemia was demonstrated (r = 0.61). All antigenemia-positive samples and randomly selected antigenemia-negative samples were subjected to IE-PCR. The results of IE-PCR showed a good correlation with those of antigenemia (r = 0.64). Furthermore, the best correlation was observed between US17-PCR and IE-PCR (r = 0.83). In conclusion, both real-time PCR methods showed a good correlation with the antigenemia assay, and could be used to monitor CMV infection after hematopoietic stem cell transplantation.
Tanaka
Y
Y
Department of Hematology, Tokyo Metropolitan Komagome Hospital, Tokyo, Japan.
Kanda
Y
Y
Kami
M
M
Mori
S
S
Hamaki
T
T
Kusumi
E
E
Miyakoshi
S
S
Nannya
Y
Y
Chiba
S
S
Arai
Y
Y
Mitani
K
K
Hirai
H
H
Mutou
Y
Y
D000956:Antigens, Viral / Q000097:blood*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000097:blood*; D017781:Genes, Immediate-Early / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D017874:Immediate-Early Proteins / Q000097:blood / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D012698:Serologic Tests; D019562:Viral Load / Q000379:methods
D000956:Antigens, Viral; D004279:DNA, Viral; D017874:Immediate-Early Proteins