title: HP (2-20) derived from the amino terminal region of helicobacterpylori ribosomal protein L1 exerts its antifungal effects by damaging the plasma membranes of Candida albicans.
abstract: The fungicidal effects of the peptide HP (2-20). derived from the N-terminal sequence of Helicobacter pylori ribosomal protein L1 (RPL1). have been investigated. HP (2-20) displays a strong fungicidal activity against various fungi, without haemolytic activity against human erythrocyte cells, and the fungicidal activity is inhibited by Ca2+ and Mg2+ ions. In order to investigate the fungicidal mechanism(s) of HP (2-20). the amount of intracellular trehalose was measured in C. albicans. It was found that the amounts of intracellular trehalose were decreased when HP (2-20) was used. The action of the peptide against fungal cell membranes was further examined by the potassium-release test; HP (2-20) was found to increase the amount of K+ released from the cells. Furthermore, HP (2-20) caused significant morphological changes, as shown by scanning electron microscopy, and by testing the membrane disrupting activity using liposomes (phosphatidyl choline/cholesterol; 10: 1, w/w). Our results suggest that HP (2-20) may exert its antifungal activity by disrupting the structure of cell membranes, via pore formation or direct interaction with the lipid bilayers.
authors.affiliation: Research Center for Proteineous Materials, Chosun University, Kwangju, Korea.
authors.forename: Dong Gun
authors.initials: DG
authors.lastname: Lee
authors.forename: Pyoung Il
authors.initials: PI
authors.lastname: Kim
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Park
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Jang
authors.forename: Seong-Cheol
authors.initials: SC
authors.lastname: Park
authors.forename: Eun-Rhan
authors.initials: ER
authors.lastname: Woo
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Hahm
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000648:ultrastructure; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D016480:Helicobacter pylori / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D008555:Melitten / Q000737:chemistry / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D012269:Ribosomal Proteins / Q000737:chemistry
chemical_list: D000935:Antifungal Agents; D001426:Bacterial Proteins; C433783:Hp (2-20) protein, Helicobacter pylori; D010446:Peptide Fragments; D012269:Ribosomal Proteins; C028546:ribosomal protein L1; D008555:Melitten