title: Decomposition of field-induced transmembrane potential responses of single cardiac cells.
abstract: In this study, we used a multi-site optical mapping system to record field-induced responses of single cells isolated from guinea pig hearts. The cells were stained with voltage sensitive dye di-8-ANEPPS and stimulated with two uniform field (S1-S2) pulses along their longitudinal axes. The first pulse (S1 = 5 ms, <10 V/cm) was applied during rest and elicited an action potential. The second pulse (S2 = 10 ms, 4-50 V/cm) was applied 15 ms after the break of the S1 pulse (during the action potential plateau). The transmembrane potential responses, Vm(F)s, were optically recorded from up to 12 sites along the cell length using a fiber optic based optical mapping system at a resolution of 17 or 25 microm. The field-induced Vm(F)s had a complex spatio-temporal pattern. We show that these responses can be decomposed into simpler components. The first component, termed the differential-mode component (Vmd(F)), is like the response of a passive cell. The second component, termed the common-mode component (Vmc(F)), is identical all along the cell and adds a constant offset to the differential mode response of various sites along the cell length, to produce the total Vm(F) responses of the cell.
authors.affiliation: vinod.sharma@medtronic.com
authors.forename: Vinod
authors.initials: V
authors.lastname: Sharma
authors.forename: Steven N
authors.initials: SN
authors.lastname: Lu
authors.forename: Leslie
authors.initials: L
authors.lastname: Tung
mesh_terms: D000465:Algorithms; D000818:Animals; D004558:Electric Stimulation / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D004574:Electromagnetic Fields; D004594:Electrophysiology; D005071:Evoked Potentials / Q000502:physiology*; D005456:Fluorescent Dyes; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000502:physiology*; D008955:Models, Cardiovascular; D008962:Models, Theoretical; D009206:Myocardium; D011726:Pyridinium Compounds; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016276:Ventricular Function
chemical_list: C089669:1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds