title: Association between HaeIII polymorphism of scavenger receptor class B type I gene and plasma HDL-cholesterol concentration.
abstract: BACKGROUND
  Evidence has recently been found for significant associations between genetic variation within the scavenger receptor class B type I gene (SR-BI), plasma lipids and anthropometric measurements in healthy Caucasians. The present case-control study was conducted to determine whether there is an association between three polymorphisms identified by the restriction endonucleases HaeIII, AluI and ApaI of SR-BI and coronary artery disease (CAD) in Korean subjects.
  
  
  METHODS
  DNA was extracted from 137 subjects with CAD and 124 age-matched controls; it was amplified using the polymerase chain reaction. Individual alleles at each of the three polymorphic sites were identified by digestion with the appropriate restriction enzyme.
  
  
  RESULTS
  Only a single allele was identified at the AluI and ApaI polymorphic sites. The frequency of the common (+) allele at the HaeIII polymorphic site was higher in CAD patients than in the controls (P = 0.001). The concentrations of plasma HDL-cholesterol and apolipoprotein AI also varied significantly among HaeIII genotypes in the CAD patients. The common (+) allele of the HaeIII polymorphism was associated with a lower body mass index in female controls.
  
  
  CONCLUSIONS
  Allele frequencies of the AluI and ApaI polymorphisms in this study were different to those in a Caucasian population studied previously, suggesting a difference in the genetic background. Further comparative studies of SR-BI polymorphism in other racial or ethnic groups should therefore prove to be of value.
authors.affiliation: Department of Science Education, JeJu National University of Education, JeJu, Korea.
authors.forename: Seung Ho
authors.initials: SH
authors.lastname: Hong
authors.forename: Young-Ree
authors.initials: YR
authors.lastname: Kim
authors.forename: Yeo Min
authors.initials: YM
authors.lastname: Yoon
authors.forename: Won Ki
authors.initials: WK
authors.lastname: Min
authors.forename: Sa Il
authors.initials: SI
authors.lastname: Chun
authors.forename: Jin Q
authors.initials: JQ
authors.lastname: Kim
mesh_terms: D000368:Aged; D000483:Alleles; D000704:Analysis of Variance; D018955:CD36 Antigens / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000097:blood*; D003327:Coronary Disease / Q000097:blood / Q000235:genetics*; D003710:Demography; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005787:Gene Frequency; D006579:Heterozygote; D006801:Humans; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B
chemical_list: D018955:CD36 Antigens; D008076:Cholesterol, HDL; D008565:Membrane Proteins; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C495124:SCARB1 protein, human; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases; C038485:GGGCCC-specific type II deoxyribonucleases