title: Tissue-specific association of the human tyrosine hydroxylase gene with the nuclear matrix.
abstract: Association of the human tyrosine hydroxylase (TH) gene with the nuclear matrix was studied using bovine matrices from tissues differing in expression of the gene. An in vitro binding assay was performed with fragments encompassing the -2300/+2300 region of the human TH gene. All fragments bound to the nuclear matrix from the adrenal medulla but none bound to that from the liver. A computer search of the sequenced part of the studied region revealed putative matrix attachment regions (S/MAR) - one in the promoter and two in the first intron. The (TCAT)(n) microsatellite repeat included in one of the intronic S/MARs seems to influence the binding potential of the human TH gene to the nuclear matrices.
authors.affiliation: N. Copernicus University, Institute of General and Molecular Biology, Laboratory of Genetics, Gagarina 9, 87 100 Toruń, Poland. rlenart@biol.uni.torun.pl
authors.forename: Robert
authors.initials: R
authors.lastname: Lenartowski
authors.forename: Anna
authors.initials: A
authors.lastname: Goc
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000235:genetics; D001667:Binding, Competitive; D015139:Blotting, Southern; D002417:Cattle; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D015530:Nuclear Matrix / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics* / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; D004247:DNA; D014446:Tyrosine 3-Monooxygenase