title: [Characterization of peptide and amino acid profile in casein hydrolysates].
abstract: The nutritional quality of protein hydrolysates has been related in several reports to their di- and tripeptide contents. In the present work different hydrolytic conditions were tested using papain in order to prepare casein hydrolysates with a suitable peptide profile for being used in special diets. The hydrolysates were fractionated by size-exclusion HPLC and the rapid Correct Fraction Area method was used for quantifying the peptides. Among the five hydrolytic conditions studied, three of them gave rise to preparations having nutritionally similar peptide profiles. However, the use of the temperature of 37 degrees C and enzyme:substrate ratio (E:S) of 2% may probably be the most economical condition for industrial production.
authors.affiliation: Laboratório de Bromatologia Faculdade de Farmácia da UFMG, Av. Olegário Maciel-Belo Horizonte-MG-Brasil.
authors.forename: Harriman Aley
authors.initials: HA
authors.lastname: Morais
authors.forename: Cristiane Márcia da Silva
authors.initials: CM
authors.lastname: Barbosa
authors.forename: Daniella Cristine
authors.initials: DC
authors.lastname: Fialho Lopes
authors.forename: Mônica Cristina
authors.initials: MC
authors.lastname: de Oliveira
authors.forename: Marialice Pinto Coelho
authors.initials: MP
authors.lastname: Silvestre
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000378:metabolism; D002364:Caseins / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009753:Nutritive Value; D010206:Papain / Q000494:pharmacology; D010455:Peptides / Q000032:analysis* / Q000378:metabolism; D011492:Protein Hydrolysates / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D014364:Tryptophan / Q000032:analysis / Q000378:metabolism; D014443:Tyrosine / Q000032:analysis / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002364:Caseins; D010455:Peptides; D011492:Protein Hydrolysates; C026375:trypticase-soy broth; D014443:Tyrosine; D014364:Tryptophan; D010206:Papain