title: Polyploidization and centrosome hyperamplification in inflammatory bronchi.
abstract: OBJECTIVE
  Inflammatory and tumorous bronchi were screened in order to obtain new tumor relevant cytogenetic parameters.
  
  
  METHODS
  Bronchial cells of 32 patients were cultivated by standard cell culture procedures.
  
  
  METHODS
  Tetraploidy and aneuploidy was determined by enumeration of chromosome 7 and 8 versus the number of centrosomes. The resulting data were correlated with histopathological data.
  
  
  RESULTS
  Tetra- and aneuploidy of epithelial cells were detectable in 76% of tumor cell cultures, 75% of high grade inflammatory tissues and 40% of non- and low grade-inflammatory tissues. Additionally, we observed centrosome hyper-amplification and multipolar mitoses not only in the tumor but also in the early stages of inflammation.
  
  
  CONCLUSIONS
  Inflammatory bronchi already show tumor-specific features and may consequently represent the preliminary genetic stage of cancer development in bronchi.
authors.affiliation: Department of Trauma-, Hand- and Reconstructive Surgery, Saarland University, Homburg, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Lothschütz
authors.forename: M
authors.initials: M
authors.lastname: Jennewein
authors.forename: S
authors.initials: S
authors.lastname: Pahl
authors.forename: H F
authors.initials: HF
authors.lastname: Lausberg
authors.forename: A
authors.initials: A
authors.lastname: Eichler
authors.forename: W
authors.initials: W
authors.lastname: Mutschler
authors.forename: R G
authors.initials: RG
authors.lastname: Hanselmann
authors.forename: M
authors.initials: M
authors.lastname: Oberringer
mesh_terms: D000328:Adult; D000368:Aged; D001984:Bronchial Neoplasms / Q000235:genetics* / Q000473:pathology*; D018385:Centrosome / Q000378:metabolism / Q000473:pathology*; D002869:Chromosome Aberrations; D004171:Diploidy; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007249:Inflammation / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D011123:Polyploidy; D014407:Tumor Cells, Cultured