Protein array technology: the tool to bridge genomics and proteomics.
The generation of protein chips requires much more efforts than DNA microchips. While DNA is DNA and a variety of different DNA molecules behave stable in a hybridisation experiment, proteins are much more difficult to produce and to handle. Outside of a narrow range of environmental conditions, proteins will denature, lose their three-dimensional structure and a lot of their specificity and activity. The chapter describes the pitfalls and challenges in Protein Microarray technology to produce native and functional proteins and store them in a native and special environment for every single spot on an array, making applications like antibody profiling and serum screening possible not only on denatured arrays but also on native protein arrays.
Eickhoff
Holger
H
Scienion AG, Berlin, Germany. eickhoff@scienion.com
Konthur
Zoltán
Z
Lueking
Angelika
A
Lehrach
Hans
H
Walter
Gerald
G
Nordhoff
Eckhard
E
Nyarsik
Lajos
L
Büssow
Konrad
K
D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods / Q000639:trends; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D020543:Proteome / Q000737:chemistry / Q000235:genetics*; D020539:Sequence Analysis, Protein / Q000295:instrumentation* / Q000379:methods* / Q000639:trends
D011506:Proteins; D020543:Proteome