title: Analysis of biomolecular interactions using a miniaturized surface plasmon resonance sensor.
abstract: A commercially available miniaturized surface plasmon resonance sensor has been investigated for its applicability to biological interaction analysis. The sensor was found to exhibit excellent repeatability and linearity for high-refractive index solutions and good reproducibility for the binding of proteins. Its detection limit for the monoclonal antibody M1 was found to be 2.1 fmol, which corresponds to a surface concentration of 21 pg/mm2. Simple surface immobilization procedures relying on biotin/avidin or glycoprotein/lectin chemistry have been explored. Equilibrium dissociation constants for the binding of the FLAG peptide to its monoclonal antibody (M1) and for the binding of concanavalin A to a glycoprotein have been determined. The close agreement of these measurements with values obtained by surface fluorescence microscopy and fluorescence correlation spectroscopy helps to validate the use of this device. Thus, this sensor shows promise as an inexpensive, portable, and accurate tool for bioanalytical applications in laboratory and clinical settings.
authors.affiliation: Department of Chemistry, Stanford University, 94305-5080, USA.
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Whelan
authors.forename: Thorsten
authors.initials: T
authors.lastname: Wohland
authors.forename: Lars
authors.initials: L
authors.lastname: Neumann
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
authors.forename: Brian K
authors.initials: BK
authors.lastname: Kobilka
authors.forename: Richard N
authors.initials: RN
authors.lastname: Zare
mesh_terms: D000818:Animals; D006801:Humans; D008904:Miniaturization; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000592:standards*
chemical_list: D011506:Proteins