title: Expression of the Variable Region Genes of the Monoclonal Antibodies Against Metal-bound Tetrapeptide in E. coli.
abstract: The variable region genes of the light and heavy chains obtained from three stems of McAb against metal-bound tetrapeptide were joined into a single chain by a linker. A 39 bp fragment of the N-terminal of CGRP was joined to the C-terminal of the heavy chain to constitute the Lv-linker-Hv-CGRP single chain gene which was cloned into the vector pTC01 and expressed in E. coli 71/18. The molecular weight of the expressed product was approximately 26 kD as shown by SDS-PAGE. Its expression level was about 20%-30% to he total cellular proteins. The product was a soluble protein and showed binding activity with its hapten by indirect ELISA assay.
authors.affiliation: Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Han
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Shen
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Chen
authors.forename: Cheng-Zhi
authors.initials: CZ
authors.lastname: Su