title: Tumor-related expression of alpha1,2fucosylated antigens on colorectal carcinoma cells and its suppression by cell-mediated priming using sugar acceptors for alpha1,2fucosyltransferase.
abstract: The accumulation of alpha1,2fucosylated antigens, such as Y (Fucalpha1,2Galbeta1,4 [Fucalpha1,3]GlcNAcbeta), Le(b) (Fucalpha1,2Galbeta1,3-[Fucalpha1,4]GlcNAcbeta), and H type 2 (Fucalpha1,2 Galbeta1,4GlcNAcbeta) occurs specifically within human colorectal tumor tissues and can be detected by an antifucosylated antigen antibody, such as the YB-2 antibody. In the present investigation, we found that the expression of these antigens bearing an alpha1,2-linked fucose correlated with the resistance of the tumor cells to anticancer treatments. Addition of an exogenous sugar acceptor for alpha1,2fucosyltransferase to the cell medium resulted in suppression of alpha1,2fucosylated antigen expression on the tumor cells and increased susceptibility to anticancer treatment. The increased susceptibility may be attributed to cancer cell-mediated priming by sugar acceptors for alpha1,2fucosyltransferase added to the medium.
authors.affiliation: Japan Immunoresearch Laboratories, 351-1 Nishiyokote-cho, Takasaki 370-0021, Japan. syazawa@po.sphere.ne.jp
authors.forename: Shin
authors.initials: S
authors.lastname: Yazawa
authors.forename: Toyo
authors.initials: T
authors.lastname: Nishimura
authors.forename: Munenori
authors.initials: M
authors.lastname: Ide
authors.forename: Takayuki
authors.initials: T
authors.lastname: Asao
authors.forename: Akihiko
authors.initials: A
authors.lastname: Okamura
authors.forename: Susumu
authors.initials: S
authors.lastname: Tanaka
authors.forename: Izumi
authors.initials: I
authors.lastname: Takai
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yagihashi
authors.forename: Abby R
authors.initials: AR
authors.lastname: Saniabadi
authors.forename: Naohisa
authors.initials: N
authors.lastname: Kochibe
mesh_terms: D000951:Antigens, Neoplasm / Q000276:immunology*; D000970:Antineoplastic Agents / Q000627:therapeutic use; D050260:Carbohydrate Metabolism; D002455:Cell Division; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D005643:Fucose / Q000378:metabolism*; D005647:Fucosyltransferases / Q000378:metabolism*; D006801:Humans; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D005643:Fucose; D005647:Fucosyltransferases