Involvement of nitric oxide in orthodontic tooth movement in rats.
Nitric oxide (NO) is an important regulatory molecule in bone formation and resorption. The purpose of this study was to examine the role of NO in orthodontic tooth movement in rats. We used specific inhibitors of NO synthases (NOS). Upper first molars of 9-week-old male Wistar rats were moved buccally for 21 days. The local administration of N(G)-nitro-L-arginine methyl ester. HCl (L-NAME), a general inhibitor of NOS activity, significantly reduced tooth movement. On the other hand, N(6)-(1-iminoethyl)-L-lysine. 2HCl (L-NIL), a selective inhibitor of the inducible isoform of NOS, had no effect. These results suggest that NO is an important biochemical mediator in the response of periodontal tissue to orthodontic force and is produced primarily through the activity of constitutive NOS.
Hayashi
Keigo
K
Division of Orthodontics, Department of Life-Long Oral Health Science, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
Igarashi
Kaoru
K
Miyoshi
Kotaro
K
Shinoda
Hisashi
H
Mitani
Hideo
H
D000704:Analysis of Variance; D000818:Animals; D001862:Bone Resorption / Q000503:physiopathology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D008239:Lysine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D008437:Maxilla; D008963:Molar; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013223:Statistics as Topic; D014087:Tooth Movement Techniques
D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D007527:Isoenzymes; C090663:N(6)-(1-iminoethyl)lysine; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D008239:Lysine; D019331:NG-Nitroarginine Methyl Ester