Development of a microslide agglutination assay with the aid of an inexpensive projection microscope.
A microslide agglutination assay was developed involving the mixing of 2.5 microl each of antiserum and a cell suspension of Listeria monocytogenes. Cell agglutination in the final volume of 5.0 microl was visually observed at a direct magnification of 22 x on the projection screen of an inexpensive 20 US dollar projection microscope. The procedure has the advantage of increasing by a factor of 20 the number of agglutination assays that can be performed with a given volume of antiserum with the use of an inexpensive optical projection system.
Abolmaaty
A
A
Department of Food Science, Massachusetts Agricultural Experiment Station, University of Massachusetts, Amherst, MA 01003, USA.
Levin
R E
RE
D000372:Agglutination Tests; D000818:Animals; D015373:Bacterial Typing Techniques; D008089:Listeria monocytogenes / Q000145:classification*; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*