The Purification and Renaturation of the Interleukin-2-Pseudomonas Exotoxin Fusion Protein (IL-2-PE).
The IL-2-PE fusion protein expressed in E. coli forms the inclusion body which can be isolated easily. The purity of the fusion protein was 90%-95% after washing the inclusion body with PBS containing 4 M urea and 0.5% Triton X-100 followed by chromatography on Sephacryl S-300 and DEAE-Sepharose FF. Then, parameters relative to the renaturation, including the concentrations of GSSG and L-Arg, the initiation concentrations of the protein, pH, temperature and Reaction time etc, were systematically analysed. An optimum condition for IL-2-PE renaturation was proposed.
Gao
Ji-Min
JM
The First Military Medical University, Guangzhou 510515, China.
Zheng
Zhong-Cheng
ZC
LinLai
Xin-Mei
XM
Wang
Xiao-Ning
XN