title: Comprehensive analysis of the gene expression profiles in human gastric cancer cell lines.
abstract: Gastric adenocarcinoma is one of the major malignancies worldwide. Gastric cell lines have been widely used as the model to study the genetics, pharmacology and biochemistry of gastric cancers. Here we describe a comprehensive survey of the gene expression profiles of 12 gastric carcinoma cell lines, using cDNA microarray with 43 000 clones. For comparison, we also explored the gene expression patterns of 15 cell lines derived from lymphoid, endothelial, stromal and other epithelial cancers. Expression levels of specific genes were validated through comparison to protein expression by immunohistochemistry using cell block arrays. We found sets of genes whose expression corresponds to the molecular signature of each cell type. In the gastric cancer cell lines, apart from genes that are highly expressed corresponding to their common epithelial origin from the gastrointestinal tract, we found marked heterogeneity among the gene expression patterns of these cell lines. Some of the heterogeneity may reflect their underlying molecular characteristics or specific differentiation program. Two putative gastric carcinoma cell lines were found to be B-cell lymphoma, and another one had no epithelial specific gene expression and hence was of doubtful epithelial origin. These cell lines should no longer be used in gastric carcinoma research. In conclusion, our gene expression database can serve as a powerful resource for the study of gastric cancer using these cell lines.
authors.affiliation: Department of Surgery, Beijing Cancer Hospital, Peking University School of Oncology, Beijing, China.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Ji
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Suet Yi
authors.initials: SY
authors.lastname: Leung
authors.forename: Jen-Tsan A
authors.initials: JT
authors.lastname: Chi
authors.forename: Kent Man
authors.initials: KM
authors.lastname: Chu
authors.forename: Siu Tsan
authors.initials: ST
authors.lastname: Yuen
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Annie S Y
authors.initials: AS
authors.lastname: Chan
authors.forename: Jiyou
authors.initials: J
authors.lastname: Li
authors.forename: Nina
authors.initials: N
authors.lastname: Dunphy
authors.forename: Samuel
authors.initials: S
authors.lastname: So
mesh_terms: D019070:Cell Lineage; D004847:Epithelial Cells / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D009363:Neoplasm Proteins