A preliminary evaluation of the functional significance of alpha-1-acid glycoprotein glycosylation on wound healing.
The laying down of collagen and fibrous tissue is a key process in wound healing, however excessive collagen (and glycoprotein) deposition causes hypertrophic and keloid scars, eg after burns. Collagen synthesis is increased in these scars compared with normal healing, as is collagenase activity, which controls the degradation pathway of collagen. The processes of wound healing are inextricably linked to those of the acute-phase response (APR): alpha-1-acid glycoprotein (AGP), a plasma glycoprotein that undergoes both an increase in concentration and an alteration in its glycosylation pattern during the APR. This study determined that AGP isolated from the plasma of burns patients was of an increased concentration and altered glycosylation pattern compared with normal plasma and was capable of directly interacting with type I collagen. It also had a profound effect on both collagen fibril formation and collagenase activity, to a degree dependent upon the percentage body surface area burned. Additionally, the results obtained provided the basis for predicting the formation of hypertrophic scars.
French
Deborah
D
University of Strathclyde, Department of Bioscience, Royal College Building, 204 George Street, Glasgow, G1 1XW, UK. deborah.french@strath.ac.uk
Watson
John
J
McCahill
Breeda
B
Taggart
Ian
I
Smith
Kevin D
KD
D002056:Burns / Q000097:blood; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006031:Glycosylation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009961:Orosomucoid / Q000378:metabolism*; D014945:Wound Healing
D009961:Orosomucoid; D003208:Concanavalin A; D003094:Collagen; D019158:N-Acetylneuraminic Acid