title: [Transient expression of fusion protein with a chimeric HBsAg-HSP70 construct in HepG2 cells].
abstract: OBJECTIVE
  To investigate transient expression of fusion protein with a chimeric HBsAg-HSP70 construct in HepG2 cells.
  
  
  METHODS
  Enkaryotic expression plasmids inserted HBsAg gene or chimeric HBsAg-HSP70 gene were prepared and transfected into HepG2 cells by means of cationic liposome. mRNA were detected by RT-PCR and proteins expressed in the cells were detected by immunocytochemistry 48 hours later. HBsAg in cultured supernatants and cell lysates were assayed by ELISA.
  
  
  RESULTS
  Fusion protein (HBsAg-HSP70) transient expression in HepG2 cells were confirmed by RT-PCR, immunocytochemistry or ELISA, but fusion protein was not assayed in cell cultured supernatants by ELISA.
  
  
  CONCLUSIONS
  Transfection of HepG2 cells with a chimeric HBsAg-HSP70 construct leads to express fusion protein, but it does not secrete into cell cultured supernatants.
authors.affiliation: Institute for Viral Hepatitis, Chongqing University of Medical Science, Chongqing 400010, China.
authors.forename: Zeng Wei
authors.initials: ZW
authors.lastname: Liang
authors.forename: Ying Hua
authors.initials: YH
authors.lastname: Lan
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
authors.forename: Yong Guo
authors.initials: YG
authors.lastname: Li
authors.forename: Da Chuan
authors.initials: DC
authors.lastname: Cai
mesh_terms: D001426:Bacterial Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D017325:Hepatitis B Vaccines / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D018840:HSP70 Heat-Shock Proteins; C444642:HSP70 protein, Mycobacterium tuberculosis; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA