title: Induction of nitric oxide synthase by Oldenlandia diffusa in mouse peritoneal macrophages.
abstract: Oldenlandia diffusa (OD) has been used to treat malignant tumors. In this study using mouse peritoneal macrophages, we have examined the mechanism by which OD regulates nitric oxide (NO) production. When OD (1 mg/ml) was used in combination with 10 U/ml of recombinant interferon-gamma (rIFN-gamma), there was a marked cooperative induction of NO production (36.13+/-7.12 microM) by the Griess method (nitrite). Treatment of macrophages with rIFN-gamma plus OD (1 mg/ml) caused a significant increase in tumor necrosis factor-alpha (TNF-alpha) production (4.49+/-1.43 ng/ml) by enzyme-linked immunosorbent assay. The increased production of NO and TNF-alpha from rIFN-gamma-plus OD-stimulated cells was almost completely inhibited by pretreatment with 100 microM of pyrrolidine dithiocarbamate (PDTC), an inhibitor of nuclear factor kappa B (NF-kappaB). PDTC also inhibited phosphorylation of IkappaB in rIFN-gamma-plus OD-stimulated cells. These findings demonstrate that OD increases the production of NO and TNF-alpha by rIFN-gamma-primed macrophages and suggest that NF-kappaB plays a critical role in mediating these effects of OD.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Chonbuk, South Korea. hmkim@wonkwang.ac.kr
authors.forename: Hwan-Suck
authors.initials: HS
authors.lastname: Chung
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: So-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Bong-Keun
authors.initials: BK
authors.lastname: Song
authors.forename: In-Seok
authors.initials: IS
authors.lastname: Jeong
authors.forename: Eon-Jeong
authors.initials: EJ
authors.lastname: Lee
authors.forename: Jong-Woong
authors.initials: JW
authors.lastname: Ahn
authors.forename: Seung-Hwa
authors.initials: SH
authors.lastname: Baek
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects / Q000502:physiology; D006801:Humans; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D032067:Oldenlandia; D010936:Plant Extracts / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D010936:Plant Extracts; D019001:Nitric Oxide Synthase