Immortalized renal proximal and collecting duct cell lines derived from transgenic mice harboring L-type pyruvate kinase promoters as tools for pharmacological and toxicological studies.
Targeted oncogenesis in transgenic mice, where an oncogene is placed under the control of the regulatory sequences of a cell-specific gene, has been used to derive lines of differentiated kidney epithelial cells derived from proximal or distal tubules or from the collecting duct. These renal cell lines were obtained from kidneys of transgenic mice harboring the large-T and little-t antigens placed under the control of regulatory sequences of the L-type pyruvate kinase gene. This review summarizes the main properties of these differentiated cell lines, which are useful ex vivo cell systems for pharmacological and toxicological studies.
Vandewalle
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Institut National de la Santé et de la Recherche Médicale U478, Faculté de Médecine Xavier Bichat, Paris, France. vandewal@bichat.inserm.fr
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001427:Bacterial Toxins / Q000633:toxicity; D002461:Cell Line, Transformed; D018432:Drug Resistance, Multiple; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D051379:Mice; D008822:Mice, Transgenic; D009857:Oncogenes; D011401:Promoter Regions, Genetic; D011770:Pyruvate Kinase / Q000235:genetics*
D000952:Antigens, Polyomavirus Transforming; D001427:Bacterial Toxins; D011770:Pyruvate Kinase