title: Protein array technology: the tool to bridge genomics and proteomics.
abstract: The generation of protein chips requires much more efforts than DNA microchips. While DNA is DNA and a variety of different DNA molecules behave stable in a hybridisation experiment, proteins are much more difficult to produce and to handle. Outside of a narrow range of environmental conditions, proteins will denature, lose their three-dimensional structure and a lot of their specificity and activity. The chapter describes the pitfalls and challenges in Protein Microarray technology to produce native and functional proteins and store them in a native and special environment for every single spot on an array, making applications like antibody profiling and serum screening possible not only on denatured arrays but also on native protein arrays.
authors.affiliation: Scienion AG, Berlin, Germany. eickhoff@scienion.com
authors.forename: Holger
authors.initials: H
authors.lastname: Eickhoff
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Konthur
authors.forename: Angelika
authors.initials: A
authors.lastname: Lueking
authors.forename: Hans
authors.initials: H
authors.lastname: Lehrach
authors.forename: Gerald
authors.initials: G
authors.lastname: Walter
authors.forename: Eckhard
authors.initials: E
authors.lastname: Nordhoff
authors.forename: Lajos
authors.initials: L
authors.lastname: Nyarsik
authors.forename: Konrad
authors.initials: K
authors.lastname: Büssow
mesh_terms: D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods / Q000639:trends; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D020543:Proteome / Q000737:chemistry / Q000235:genetics*; D020539:Sequence Analysis, Protein / Q000295:instrumentation* / Q000379:methods* / Q000639:trends
chemical_list: D011506:Proteins; D020543:Proteome