title: Effect of extract of medicinal plants on the labeling of blood elements with Technetium-99m and on the morphology of red blood cells: I--a study with Paullinia cupana.
abstract: Drugs can alter the labeling and the morphology of red blood cells. As Paullinia cupana is used in popular medicine, we evaluated its influence on the labeling process using technetium-99m (Tc-99m). Blood was incubated with P. cupana, stannous chloride and Tc-99m. Samples were centrifuged and plasma (P) and blood cells (BC) were separated and precipitated with trichloroacetic acid. Soluble (SF) and insoluble fractions (IF) were isolated. The morphology of the blood cells was evaluated under an optical microscope. The results showed a significant (P = 0.05) decrease in the uptake of radioactivity for the RBC (97.93 +/- 0.74 to 36.90 +/- 4.71%), in IF-P and in IF-BC due to P. cupana extract. The study of the morphology of the RBC revealed alterations in the shape of these cells. We suggest that the P. cupana effect could be explained by an inhibition of the stannous and pertechnetate ions or oxidation of the stannous ion or by damages in the plasma membrane.
authors.affiliation: Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofísica e Biometria, Av. 28 de Setembro, 87, Rio de Janeiro, RJ 20551-030, Brazil.
authors.forename: J F
authors.initials: JF
authors.lastname: de Oliveira
authors.forename: A S
authors.initials: AS
authors.lastname: Avila
authors.forename: A C S
authors.initials: AC
authors.lastname: Braga
authors.forename: M B N
authors.initials: MB
authors.lastname: de Oliveira
authors.forename: E M
authors.initials: EM
authors.lastname: Boasquevisque
authors.forename: R L
authors.initials: RL
authors.lastname: Jales
authors.forename: V N
authors.initials: VN
authors.lastname: Cardoso
authors.forename: M
authors.initials: M
authors.lastname: Bernardo-Filho
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000187:drug effects* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D015609:Organotechnetium Compounds; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D010949:Plasma / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D029631:Sapindaceae; D017971:Tin Compounds
chemical_list: D015609:Organotechnetium Compounds; D010936:Plant Extracts; D017971:Tin Compounds; C105829:technetium Tc 99m stannous chloride