Expression and characterization of flagella in nonmotile enteroinvasive Escherichia coli isolated from diarrhea cases.
We report that enteroinvasive Escherichia coli (EIEC) serotypes considered to be nonmotile produce an unusually large (77 kDa) flagellin that is assembled into functional flagellum filaments that allow the bacteria to swim in modified motility agar. The EIEC flagellin showed N-terminal identity to most common enterobacterial flagellins, especially those of the E. coli H7 serotype. These data are important in terms of the epidemiology, evolution, and biology of EIEC.
Andrade
Agda
A
Departamento de Analises Clinicas e Toxicologicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.
Girón
Jorge A
JA
Amhaz
Juliana M K
JM
Trabulsi
Luiz R
LR
Martinez
Marina B
MB
D000595:Amino Acid Sequence; D001483:Base Sequence; D003429:Cross Reactions; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology; D004755:Enterobacteriaceae / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology / Q000648:ultrastructure*; D004927:Escherichia coli Infections / Q000382:microbiology; D005407:Flagella / Q000502:physiology / Q000648:ultrastructure*; D005408:Flagellin / Q000235:genetics / Q000276:immunology; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D014774:Virulence
D004269:DNA, Bacterial; D005408:Flagellin