Transcriptional upregulation of SCG10 and CAP-23 is correlated with regeneration of the axons of peripheral and central neurons in vivo.
We have compared SCG10 and CAP-23 expression with that of GAP-43 during axonal regeneration in the peripheral and central nervous systems (PNS, CNS) of adult rats. SCG10, CAP-23, and GAP-43 mRNAs were strongly upregulated by motor and dorsal root ganglion (DRG) neurons following sciatic nerve crush, but not after dorsal rhizotomy. When the sciatic nerve was cut and ligated to prevent reinnervation of targets, expression of all three mRNAs was prolonged. Neurons in the thalamic reticular nucleus and deep cerebellar nuclei transiently upregulated these mRNAs after axotomy, and showed prolonged upregulation of all three molecules when regenerating axons into peripheral nerve grafts inserted into the thalamus of cerebellum. Neurons in the dorsal thalamus and cerebellar cortex showed poor regenerative capacity and most did not upregulate any of these mRNAs. Thus, in both PNS and CNS neurons, the transcription of SCG10, CAP-23, and GAP-43 mRNAs is coregulated following axotomy and during regeneration. Signals from living peripheral nerve appear to maintain expression of all three mRNAs in regenerating neurons, and in PNS neurons downregulation correlates with target reinnervation. Thus, SCG10 and CAP-23, as well as GAP-43, are likely to be important neuronal determinants of regenerative ability.
Mason
Matthew R J
MR
Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom.
Lieberman
A R
AR
Grenningloh
G
G
Anderson
P N
PN
D000818:Animals; D001369:Axons / Q000502:physiology*; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins; D002531:Cerebellum / Q000503:physiopathology / Q000601:surgery; D003598:Cytoskeletal Proteins / Q000235:genetics; D005260:Female; D019922:GAP-43 Protein / Q000235:genetics; D005727:Ganglia, Spinal / Q000293:injuries / Q000378:metabolism; D008565:Membrane Proteins; D008868:Microtubule Proteins; D009409:Nerve Crush; D009414:Nerve Growth Factors / Q000235:genetics* / Q000378:metabolism*; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012584:Sciatic Nerve / Q000293:injuries / Q000503:physiopathology; D013116:Spinal Cord / Q000378:metabolism; D013787:Thalamic Nuclei / Q000503:physiopathology; D013788:Thalamus / Q000378:metabolism / Q000601:surgery; D013979:Tibial Nerve / Q000378:metabolism / Q000637:transplantation; D014158:Transcription, Genetic / Q000502:physiology*; D015854:Up-Regulation; D014947:Wounds and Injuries / Q000378:metabolism
D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D019922:GAP-43 Protein; D008565:Membrane Proteins; D008868:Microtubule Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C512848:Stmn2 protein, rat; C066853:Basp1 protein, rat