title: Electroconvulsive shock increases the phosphorylation of amphiphysin II in the rat cerebellum.
abstract: Amphiphysin II (Amph2) is known to undergo rapid dephosphorylation and phosphorylation at nerve terminals. After in vivo electroconvulsive shock (ECS) in the rat cerebellum, we found an electrophoretic mobility retardation of Amph2, which suggested an increased degree of phosphorylation above the non-stimulated level. This shifted signal was observed from 1 min, reached the maximum level at 5 min and extended beyond 2 h after ECS. The shifted band was markedly decreased by the phosphatase treatment. Pretreatment with cyclosporin A augmented the mobility retardation of Amph2 after ECS. Our results indicate that ECS induces the phosphorylation of Amph2 in the rat cerebellum.
authors.affiliation: Department of Psychiatry and Behavioral Science, Seoul National University College of Medicine, Clinical Research Institute, Seoul National University Hospital and Institute for Neuroscience, Seoul National University, Seoul, South Korea.
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Koo
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Song Hee
authors.initials: SH
authors.lastname: Jeon
authors.forename: Seung Ryul
authors.initials: SR
authors.lastname: Kim
authors.forename: Ung Gu
authors.initials: UG
authors.lastname: Kang
authors.forename: Joo-Bae
authors.initials: JB
authors.lastname: Park
authors.forename: Yong Sik
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002531:Cerebellum / Q000378:metabolism*; D016572:Cyclosporine / Q000494:pharmacology; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D004597:Electroshock; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004791:Enzyme Inhibitors; D009419:Nerve Tissue Proteins; C075637:amphiphysin; D016572:Cyclosporine; D010744:Phosphoric Monoester Hydrolases