[Cloning of AE1-c-end cDNA and construction of its expression plasmid for yeast two-hybrid system].
In this study, about 350 bp cDNA fragment was amplified by PCR. After being sequenced, the AE1-c-end gene fragment was cloned into EcoR I-Pst I site of pGADT7 to form AD ends in the yeast two-hybrid system. The recombinant plasmid was transformed into yeast AH109, and the expression in the yeast was observed. The results demonstrate that AE1-c-end was obtained. pGADT7-AE1-c-end has no toxic effect on the yeast. It can serve as a target gene of yeast two-hybrid system.
Li
Hongtao
H
Department of Pathophysiology, Harbin Medical University, Harbin 150086.
Fu
Guohui
G
Qin
Yong
Y
Du
Hongqing
H
Jiang
Xiaoshu
X
Liu
Ming
M
Kong
Xiangang
X
D003001:Cloning, Molecular; D018076:DNA, Complementary; D010957:Plasmids; D016133:Polymerase Chain Reaction; D020798:Two-Hybrid System Techniques; D015003:Yeasts
D018076:DNA, Complementary