Intracellular calcium involved in the long-term potentiation induced by securinine in dentate gyrus of anesthetized rats.
The effect of nimodipine on securinine-induced long-term potentiation in rat hippocampus was studied. The rise of intracellular calcium during the induction of long-term potentiation was measured using Fura-2/AM--a Ca (2+) fluorescent chelator. Results showed that intracerebroventricular injection of nimodipine (4 nmol) blocked the induction of long-term potentiation elicited by securinine (0.4 pmol) and high-frequency stimulation (p < 0.05), but 2-amino-5-phosphonovaleric acid (5 nmol) had no inhibitory effect on securinine-induced long-term potentiation. In addition, securinine-induced intracellular calcium increment was inhibited by nimodipine (10(-6) mol. L(-1), p < 0.01), but not by 2-amino-5-phosphonovaleric acid (10(-6) mol. L(-1), p > 0.05).
Lin
Xu
X
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Guolin
Wen
W
Jun-tian
Zhang
Z
D000470:Alkaloids / Q000494:pharmacology*; D000758:Anesthesia; D000818:Animals; D001381:Azepines; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D018891:Dentate Gyrus / Q000187:drug effects* / Q000378:metabolism*; D004347:Drug Interactions; D019665:Euphorbiaceae / Q000737:chemistry; D006576:Heterocyclic Compounds, 4 or More Rings; D006572:Heterocyclic Compounds, Bridged-Ring; D007783:Lactones; D017774:Long-Term Potentiation / Q000187:drug effects*; D008297:Male; D015394:Molecular Structure; D009553:Nimodipine / Q000494:pharmacology; D010880:Piperidines; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D000470:Alkaloids; D001381:Azepines; D002121:Calcium Channel Blockers; D006576:Heterocyclic Compounds, 4 or More Rings; D006572:Heterocyclic Compounds, Bridged-Ring; D007783:Lactones; D010880:Piperidines; D009553:Nimodipine; C000785:securinine; D002118:Calcium