title: Cloning and expression of the exo-beta-D-1,3-glucanase gene (exgS) from Aspergillus saitoi.
abstract: A gene of exo-1,3-beta-D-glucanase (exgS) was cloned from a koji mold, Aspergillus saitoi, genomic DNA using PCR. The exgS has an ORF comprising 2832 bp, which contains one intron of 45 bp, and encodes 945 amino acids. The deduced amino acid sequences showed that the ExgS has a non-homologous linker region consisting of 180 amino acids, which encompassed highly conserved regions observed in Exg homologues from filamentous fungi. A recombinant protein (ExgS) has been recovered from the cultural filtrate of an Aspergillus oryzae strain that carried an expression vector containing full length of the exgS. The N-terminal amino acid sequences of the recombinant exo-1,3-beta-D-glucanase (ExgS) were identical to that of native ExgS from A. saitoi.
authors.affiliation: Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. odaken@biochem.tohoku.ac.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Oda
authors.forename: Shin
authors.initials: S
authors.lastname: Kasahara
authors.forename: Youhei
authors.initials: Y
authors.lastname: Yamagata
authors.forename: Keietsu
authors.initials: K
authors.lastname: Abe
authors.forename: Tasuku
authors.initials: T
authors.lastname: Nakajima
mesh_terms: D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C021780:exo-1,3-alpha-glucanase