Cold shock induction of the cspL gene in Lactobacillus plantarum involves transcriptional regulation.
Fragments of the cspL promoter region were fused to the gusA reporter and reintroduced into Lactobacillus plantarum cells, either on multicopy plasmids or through single-copy chromosomal integration. beta-Glucuronidase activity and primer extension data demonstrate that the cspL promoter is induced in response to cold shock and that multicopy constructs quench the induction of the resident cspL gene.
Derzelle
Sylviane
S
Institut des Sciences de la Vie/Unité de Génétique, Université Catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium.
Hallet
Bernard
B
Ferain
Thierry
T
Delcour
Jean
J
Hols
Pascal
P
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003080:Cold Temperature; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C105798:CspL protein, Lactobacillus plantarum; D006360:Heat-Shock Proteins; D011993:Recombinant Fusion Proteins; D005966:Glucuronidase