title: Separating lysozyme from bacteriophage P22 in two-phase aqueous micellar systems.
abstract: This communication demonstrates that two-phase aqueous mixed (nonionic/ionic) micellar systems have the potential for improving the separation of proteins from viruses. Specifically, two separation experiments were performed to show that the addition of the anionic surfactant sodium dodecyl sulfate (SDS) to the two-phase aqueous nonionic n-decyl tetra(ethylene oxide) (C(10)E(4)) micellar system increases the yield of a model net positively charged protein, lysozyme, in the micelle-rich phase from 75 to 95%, while still maintaining approximately the same yield of a model net negatively charged virus, bacteriophage P22, in the micelle-poor phase (97% vs. 98%).
authors.affiliation: Department of Chemical Engineering, Room 66-444, MIT, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Kamei
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: King
authors.forename: Daniel I C
authors.initials: DI
authors.lastname: Wang
authors.forename: Daniel
authors.initials: D
authors.lastname: Blankschtein
mesh_terms: D017100:Bacteriophage P22 / Q000737:chemistry / Q000302:isolation & purification*; D002460:Cell Line; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D008823:Micelles; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000235:genetics / Q000821:virology; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D055672:Static Electricity; D014780:Viruses / Q000302:isolation & purification; D014867:Water / Q000737:chemistry
chemical_list: D008823:Micelles; D011506:Proteins; D014867:Water; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; C069485:decyltetraoxyethylene glycol monoether; D009113:Muramidase