title: Protein Engineering of Insulin: [B9Glu, B10Asp] Human Insulin.
abstract: B9Ser and B10His of the insulin B chain are substituted respectively by Glu and Asp using a gapped duplex DNA approach for site-directed mutagenesis. A mutant insulin-[B9Glu, B10Asp] human insulin was obtained. The receptor binding capacity of the mutant insulin is 34.4% as that of porcine insulin. However, the in vivo biological activity of [B9Glu, B10Asp] human insulin is almost as the same as that of porcine insulin.
authors.affiliation: State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
authors.forename: Zhen-He
authors.initials: ZH
authors.lastname: Liang
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Tang
authors.forename: You-Min
authors.initials: YM
authors.lastname: Feng