title: [Effects of serum-free murine bone marrow endothelial cell conditioned medium on the growth of CFU-E and BFU-E].
abstract: Serum-free murine bone marrow endothelial cell conditioned medium(mBMEC-cm) was collected. The mBMEC-cm was ultrafiltered in Centriprep. The concentrated retentive substance of mBMEC-cm (MW > 10,000) and filtrate of mBMEC-cm(MW < 10,000) were obtained. The effects of mBMEC-cm on the growth of CFU-E and BFU-E were investigated. The results showed: 1. The retentive substance of mBMEC-cm significantly enhanced the growth of CFU-E and BFU-E. The effects of mBMEC-cm(2%-6%, V/V) on the growth of CFU-E and BFU-E were dose-dependent. 2. The filtrate of mBMEC-cm markedly inhibited the growth of CFU-E and BFU-E. The effects of the filtrate of mBMEC-cm on the growth of CFU-E and BFU-E were also dose-dependent. The mechanism of regulation will be studied further.
authors.affiliation: Research Laboratory of Blood Physiology, Hunan Medical University, Changsha 410078.
authors.forename: Y H
authors.initials: YH
authors.lastname: Huang
authors.forename: W M
authors.initials: WM
authors.lastname: Li
authors.forename: D Z
authors.initials: DZ
authors.lastname: Jiang
authors.forename: Q R
authors.initials: QR
authors.lastname: Wang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002455:Cell Division; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016895:Culture Media, Serum-Free; D004727:Endothelium / Q000166:cytology*; D015672:Erythroid Precursor Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free