Expression of Human Bone Morphogenetic Protein 2 in Bombyx mori Larvae and Its Purification.
Recombinant Bm-BacPAK-BMP2 virus which contains the human bone morphogenetic protein 2 (BMP2) cDNA gene under the control of polyhedrin gene promoter was generated by cotransfection into Bm-N cells with the transfer plasmid pBacPAK-BMP2 and the modified Bombyx mori nuclear polyhedrosis virus Bm-BacPAK genomic DNA. On the fifth day after infection with the recombinant virus, Bombyx mori larvae produced about 10 &mgr;g rBMP2 in 1 ml hemolymph. The expressed rBMP2 was processed into 16 kD C-terminal fragments and formed 30 kD dimmers with disulfide bonds in vivo. The protein was purified to about 90% purity and induced cartilage locally in rats 7 days after subcutaneous transplantation.
Lin
Wong
W
Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
Mi
Yi-De
YD
Dai
Nie-Hong
NH
Wu
Xiang-Fu
XF
Li
Bo-Liang
BL