Detection of epstein-barr virus in nasal T-cell lymphoma.
The close relationship between Epstein-Barr virus (EBV) and nasal T-cell lymphoma (NTL) has frequently been reported. However, the status of the infection, either lytic or latent, is obscure. This study involved 16 patients with NTL. Phenotypes of lymphoma cells were examined by immunohistochemical staining using CD3, CD4, CD8, CD20 and CD45RO monoclonal antibodies. EBV-encoded small nuclear RNA (EBER)-1 and EBV NotI tandem repeat region were detected by reverse transcription, using a rapid (< or = 60 min) in situ hybridization technique. Tumor cells expressed at least one T-cell marker, such as CD3, CD4, CD8 and CD45RO. CD20 was not detected in any of the cases. EBER-1 was identified in all cases; no Notl tandem DNA repeat was demonstrated. All cases demonstrated a T-cell phenotype. These data suggest that NTL is associated with EBV infection in the latent phase.
Makoshi
Tomohiro
T
Takahashi
Hiroomi
H
Kameya
Toru
T
Yao
Kazuo
K
Sato
Yuichi
Y
Nakayama
Meijin
M
Tsuchiya
Benio
B
Okamoto
Makito
M
D015703:Antigens, CD / Q000032:analysis / Q000235:genetics; D020031:Epstein-Barr Virus Infections / Q000235:genetics* / Q000382:microbiology / Q000473:pathology*; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D017403:In Situ Hybridization; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000382:microbiology / Q000473:pathology*; D009296:Nasal Cavity / Q000382:microbiology* / Q000473:pathology*; D009669:Nose Neoplasms / Q000235:genetics* / Q000382:microbiology / Q000473:pathology*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020080:Tandem Repeat Sequences / Q000235:genetics
D015703:Antigens, CD; C099537:Epstein-Barr virus encoded RNA 1; D012367:RNA, Viral