[12S rRNA, cytochrome b and MDR1 gene DNA sequence and phylogenetic evolution of Muntiacus (M. muntjak, M. reevesi, M. crinifros)].
In the paper, 784 bp-DNA fragmensts of 12S rRNA and cytochrome b gene on Mitochondrial DNA and 728 bp-DNA fragments of MDR1 (multidrug resistance) gene from Muntiacus (M. muntjak, M. reevesi, M. crinifrons) were amplified and sequenced, while their phylogenetic relationships and classification were analysed. The molecular phylogenetic trees were constructed based on these combined DNA sequences, the results suggested that DIST is 0.042 between M. crinifrons and M. muntjak; DIST is 0.047 between M. crinifrons and M. reevesi; DIST is 0.055 between M. muntjak and M. reevesi, counted according to the evolution speed of 2.5% per million year, the divergent time between M. crinifrons and M. muntjak is 1.68 million year; the divergent time between M. crinifrons and M. reevesi is 1.88 million year; the divergent time between M. muntjak and M. reevesi is 2.2 million year; The conclusion is that the M. crinifrons and M. reevesi were diverged from an ancestor analogied with M. muntjak, M. nuntjak is the oldest species, M. crinifrons and M. reevesi are newer ones.
Yi
Guang-Cai
GC
College of Life Sciences, Nanjing Normal University, Nanjing 210097, China.
Zhang
Xiao-Mei
XM
Shan
Xiang-Nian
XN
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D018668:Muntjacs / Q000235:genetics*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D003573:Cytochrome b Group; D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D004247:DNA