[Comparative study on livertaxis of lactosaminated human growth hormone and galactosyl-human growth hormone].
This study was conducted to compare the livertaxis of lactosaminated human growth hormone (hGH-L) with that of galactosyl human growth hormone (hGH-Gal), using radioactive tracer technique. hGH-L and hGH-Gal were first prepared. After hGH-L, hGH-Gal and hGH were labelled with 131I or 125I, the data on biodistribution in mice, imaging in rabbits and chicken were obtained. hGH-L and hGH-Gal had remarkable livertaxis and their percentages for uptake in liver were 68.83%-74.65% and 68.18%-74% respectively, which were about two times as high as that of hGH. All of the hepatic bindings were receptor-mediated. But, the synthesizing of hGH-L was more easy-to-do, more mild in reaction conditions, and more economical of raw material as compared with the synthesizing of hGH-Gal. Therefore, hGH-L is a more potential receptor-mediated hepatic targeting secreting somatomedian drug for treating dwarfism.
Li
Y
Y
Department of Nuclear Medicine, First Affiliated Hospital, WCUMS, Chengdu 610041.
Guan
C
C
Zhang
H
H
Li
L
L
Yang
X
X
Chen
C
C
Li
T
T
Pang
Q
Q
D000818:Animals; D037263:Asialoglycoprotein Receptor / Q000378:metabolism; D001212:Asialoglycoproteins / Q000378:metabolism; D002645:Chickens; D004393:Dwarfism, Pituitary / Q000188:drug therapy; D019382:Human Growth Hormone / Q000145:classification / Q000493:pharmacokinetics*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D051379:Mice; D011817:Rabbits; D011897:Random Allocation
D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D019382:Human Growth Hormone