title: Characterisation of a hydroxymandelate oxidase involved in the biosynthesis of two unusual amino acids occurring in the vancomycin group of antibiotics.
abstract: ORF22 from the chloroeremomycin gene cluster has been cloned, expressed and characterised as a hydroxymandelate oxidase (HmO) that is involved in the formation of both (S)-4-hydroxyphenylglycine and (S)-3,5-dihydroxyphenylglycine.
authors.affiliation: Cambridge Centre for Molecular Recognition, Department of Chemistry, Lensfield Road, Cambridge, UK CB2 1EW.
authors.forename: T L
authors.initials: TL
authors.lastname: Li
authors.forename: O W
authors.initials: OW
authors.lastname: Choroba
authors.forename: E H
authors.initials: EH
authors.lastname: Charles
authors.forename: A M
authors.initials: AM
authors.lastname: Sandercock
authors.forename: D H
authors.initials: DH
authors.lastname: Williams
authors.forename: J B
authors.initials: JB
authors.lastname: Spencer
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000596:Amino Acids / Q000096:biosynthesis*; D001426:Bacterial Proteins; D002849:Chromatography, Gas; D015964:Gene Expression Regulation, Bacterial; D005998:Glycine / Q000031:analogs & derivatives* / Q000096:biosynthesis*; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015394:Molecular Structure; D010088:Oxidoreductases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012118:Resorcinols; D013053:Spectrophotometry; D014640:Vancomycin / Q000031:analogs & derivatives* / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D008565:Membrane Proteins; C435514:ORF22 protein, Corynebacterium glutamicum; D012118:Resorcinols; C112142:chloroeremomycin; C079215:3,5-dihydroxyphenylglycine; D014640:Vancomycin; C023010:4-hydroxyphenylglycine; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C021538:L-mandelate dehydrogenase; D005998:Glycine