A novel gene, NMES1, downregulated in human esophageal squamous cell carcinoma.
To isolate genes with different expression levels in human esophageal SCC, SSH and reverse Northern were performed between cancer tissue and its normal counterpart. Among the differentially expressed genes identified, we report here cDNA corresponding to a 0.88 kb mRNA (NMES1), whose expression was observed in all 36 adjacent normal esophageal mucosae, while 31 (86%) matched cancer tissues showed a marked reduction or complete lack of its expression. Sequence analysis of its full-length cDNA revealed a gene encoding a predicted polypeptide of 9 kDa. Northern blot showed that NMES1 was distributed mainly in the alimentary tract. The gene was mapped to 15q21.1 by screening the GenBridge 4 RH panel. Immunohistochemistry confirmed the downregulation of NMES1 in esophageal SCC at the protein level and showed that it is a nuclear protein. In situ hybridization revealed its different expression during mouse embryonic development, especially in bone, brain, stomach and intestine. The negative correlation of NMES1 expression with esophageal oncogenesis suggests its suppressive role in tumorigenesis of the esophagus, while the precise function of NMES1 still needs further investigation.
Zhou
Jin
J
National Laboratory of Molecular Oncology, Department of Cell Biology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Wang
Huixin
H
Lu
Ailing
A
Hu
Gengxi
G
Luo
Aiping
A
Ding
Fang
F
Zhang
Jian
J
Wang
Xiuqin
X
Wu
Min
M
Liu
Zhihua
Z
D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D018076:DNA, Complementary / Q000302:isolation & purification; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D009687:Nuclear Proteins; D009693:Nucleic Acid Hybridization; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
D018076:DNA, Complementary; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; C465665:C15orf48 protein, human