[Determination of astragaloside IV in radix Astragali by HPLC with evaporative light scattering detector].
OBJECTIVE
To develop a new method for the determination of astragaloside IV in Radix Astragali.


METHODS
A HPLC-ELSD method was set up, using Hypersil C18 column (150 mm x 4.6 mm), acetonitrile-water(1:2) as mobile phase with a flow rate of 1.0 ml.min-1. The parameters of drift tube and gas flow rate of the detector were set at 105 degrees C and 2.96 L.min-1 respectively.


RESULTS
The calibration curve was linear in the range of 2.02-10.12 micrograms. The average recovery was 100.5%.


CONCLUSIONS
The active constituent astragaloside IV in Radix Astragali can be separated effectively. ELSD detection is appropriate for the determination of constituents with end absorption at low ultra-violet wavelengths.
Zhou
C L
CL
Liaoning Provincial Institute for Drug Control, Shenyang 110023, Liaoling, China.
Lu
J
J
D027885:Astragalus propinquus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000032:analysis*; D014315:Triterpenes / Q000032:analysis*
D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A