Overexpression of human thioredoxin in transgenic mice controls oxidative stress and life span.
Transgenic (Tg) mice overexpressing human thioredoxin (TRX), a small redox-active protein, were produced to investigate the role of the protein in a variety of stresses. Bone marrow cells from TRX-Tg mice were more resistant to ultraviolet C-induced cytocide compared with those from wild type (WT) C57BL/6 mice. TRX-Tg mice exhibited extended median and maximum life spans compared with WT mice. Telomerase activity in spleen tissues in TRX-Tg mice was higher than that in WT mice. These results suggest that overexpression of TRX results in resistance against oxidative stress and a possible extension of life span without apparent abnormality in mammals.
Mitsui
Akira
A
Pharmaceutical Research Laboratories, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki, 210-8681, Japan.
Hamuro
Junji
J
Nakamura
Hajime
H
Kondo
Norihiko
N
Hirabayashi
Yoko
Y
Ishizaki-Koizumi
Sonoko
S
Hirakawa
Tadashi
T
Inoue
Tohru
T
Yodoi
Junji
J
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000528:radiation effects; D001921:Brain / Q000378:metabolism; D006801:Humans; D008136:Longevity; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress; D013154:Spleen / Q000201:enzymology; D015996:Survival Rate; D019098:Telomerase / Q000378:metabolism; D013879:Thioredoxins / Q000235:genetics* / Q000378:metabolism*; D014466:Ultraviolet Rays
D013879:Thioredoxins; D019098:Telomerase