Significant quantities of the glycolytic enzyme phosphoglycerate mutase are present in the cell wall of yeast Saccharomyces cerevisiae.
NaOH was used to extract proteins from the cell walls of the yeast Saccharomyces cerevisiae. This treatment was shown not to disrupt yeast cells, as NaOH-extracted cells displayed a normal morphology upon electron microscopy. Moreover, extracted and untreated cells had qualitatively similar protein contents upon disruption. When yeast was grown in the presence of 1 M mannitol, two proteins were found to be present at an elevated concentration in the cell wall. These were found to be the late-embryogenic-abundant-like protein heat-shock protein 12 and the glycolytic enzyme phosphoglycerate mutase. The presence of phosphoglycerate mutase in the cell wall was confirmed by immunocytochemical analysis. Not only was the phosphoglycerate mutase in the yeast cell wall found to be active, but whole yeast cells were also able to convert 3-phosphoglycerate in the medium into ethanol, provided that the necessary cofactors were present.
Motshwene
Precious
P
Department of Molecular and Cellular Biology, University of Cape Town, Private Bag, Rondebosch 7701, South Africa.
Brandt
Wolf
W
Lindsey
George
G
D002473:Cell Wall / Q000201:enzymology*; D005988:Glyceric Acids / Q000378:metabolism; D007150:Immunohistochemistry; D008353:Mannitol / Q000378:metabolism; D010736:Phosphoglycerate Mutase / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012972:Sodium Hydroxide / Q000378:metabolism
D005988:Glyceric Acids; D029701:Saccharomyces cerevisiae Proteins; D008353:Mannitol; D012972:Sodium Hydroxide; C005156:3-phosphoglycerate; D010736:Phosphoglycerate Mutase