Glutathione determination and a study of the activity of glutathione-peroxidase, glutathione-transferase, and glutathione-reductase in renal transplants.
The aim of this work is to study the temporary variation of oxidative stress in renal transplants, both in plasma and in erythrocytes (CR). In order to do so, we determined total glutathione (GST) levels, both oxidized (GSSG) and reduced (GSH), and the activity of enzymes, glutathione peroxidase (G-px), glutathione reductase (G-red) and glutathione transferase (GSt), in renal transplant patients. Determinations were made 48 h before the transplant 1 week and 2 weeks after the renal transplant. The results obtained confirm a high "oxidative stress" rate, resulting from the equilibrium between the production of free radicals and the activity of antioxidants, the former being higher proportionally. Immediately after the transplant there is an increase of oxidative stress, which results in an increase of G-red, a marked decrease of G-px in plasma and in erythrocytes (CR) and an abrupt drop both in GST levels in plasma and in GSG (as well as in the [GSH]/[GSSG] relationship). As times goes on, after the transplant, there is a significant improvement in the activity of antioxidant enzymes, but there is no normalization, which is easily seen in the fact that total glutathione levels and the activity of the various enzymes approach the average values of the control group.
De Vega
L
L
Department of Biochemistry, School of Science, University of Valladolid, Spain.
Férnandez
R Pérez
RP
Mateo
M C Martin
MC
Bustamante
J Bustamante
JB
Herrero
A Mendiluce
AM
Munguira
E Bustamante
EB
D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005978:Glutathione / Q000097:blood*; D005979:Glutathione Peroxidase / Q000097:blood*; D005980:Glutathione Reductase / Q000097:blood*; D005982:Glutathione Transferase / Q000097:blood*; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D018384:Oxidative Stress / Q000502:physiology
D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione