Optimizing lipases and related enzymes for efficient application.
Although numerous reactions have been performed using lipases and related enzymes (e.g. esterases and phospholipases), it is still a challenge to identify the most suitable biocatalyst and best reaction conditions for an efficient application. Frequently used methods such as immobilization and optimization of the reaction medium cannot be transferred from one reaction system or substrate to another. However, in the past few years, rational protein design and directed evolution have emerged as efficient alternative methods to optimize biocatalytic reactions.
Bornscheuer
Uwe T
UT
Institute of Chemistry & Biochemistry, Department of Technical Chemistry & Biotechnology, Greifswald University, Soldmannstr. 16, D-17487 Greifswald, Germany. uwe.bornscheuer@uni-griefswald.de
Bessler
Cornelius
C
Srinivas
Ramisetti
R
Krishna
Sajja Hari
SH
D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D030562:Databases, Protein; D019020:Directed Molecular Evolution / Q000379:methods*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D005658:Fungi / Q000201:enzymology* / Q000235:genetics; D008049:Lipase / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D013045:Species Specificity
D004800:Enzymes, Immobilized; D008049:Lipase