Fluorescent transformation markers for insect transgenesis.
The first effectively achieved germ-line transformations of non-drosophilid insects were based on mutant rescue of eye color phenotypes. However, for most insect species neither visible mutants nor corresponding cloned genes are available. Therefore, the development of broadly applicable and reliable transformation markers will be of great importance to fully exploit the enormous potential transgenic insect technology has to offer. Here we review transposon-mediated germ-line transformation approaches that employ green fluorescent protein (GFP) variants to identify successful gene transfer. Furthermore, we provide novel data on the use of DsRed as an additional red fluorescent transformation marker for insect transgenesis. In conclusion, fluorescent proteins controlled by suitable strong promoters possess ideal characteristics to serve as transformation markers for a wide range of insect species.
Horn
Carsten
C
Universität Bayreuth, Lehrstuhl für Genetik, Universitätsstrasse 30 NWI, 95447 Bayreuth, Germany.
Schmid
Bernhard G M
BG
Pogoda
Frank S
FS
Wimmer
Ernst A
EA
D000818:Animals; D030801:Animals, Genetically Modified; D005127:Eye Color; D005453:Fluorescence; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D007313:Insecta / Q000235:genetics*; D008164:Luminescent Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins