Production of long-chain levan by a sacC insertional mutant from Bacillus subtilis 327UH.
A hyper extracellular protein producer, Bacillus subtilis 327UH, produced large amounts of levan in a medium containing 20% sucrose, and the yield of levan after 10 hours was more than 60%, when based on the fructose amount of sucrose. After transformation of 327UH with a levanase-deficient 168SC (sacC::Cm(r)) chromosomal DNA, a Cm(r) transformant 327UHSC (sacC::Cm(r) degSU(Hy)) produced 3 times longer levan than that of the wild type.
Shida
Toshio
T
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.
Mukaijo
Kohsuke
K
Ishikawa
Shu
S
Yamamoto
Hiroki
H
Sekiguchi
Junichi
J
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005630:Fructans / Q000096:biosynthesis* / Q000737:chemistry; D005798:Genes, Bacterial / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000172:deficiency / Q000235:genetics; D016254:Mutagenesis, Insertional; D009154:Mutation / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D013395:Sucrose; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D005630:Fructans; D013395:Sucrose; D006026:Glycoside Hydrolases; C021512:levanase