DNA separation at a liquid-solid interface.
We demonstrate that it is possible to separate a broad band of DNA on a solid substrate without topological obstacles. The mobility was found to scale with molecular size (N) as N(-0.25), while the resolution scaled as N(0.75) indicating that diffusivity on this substrate was minimal. By varying the buffer concentration we were able to show that the mobility for a given chain length scaled with the persistent length (p) as p(1/2). This could be shown to be related to the Gaussian conformation of the chains adsorbed on the surface. A two-dimensional corrugated surface of nonporous silica beads was produced using a self-assembling process at the air/water interface. Even though the surface corrugations were comparable to persistence length we show that they do not affect the mobility, indicating that surface friction rather than topological constraints are the predominant mechanism of separation on a surface.
Seo
Young-Soo
YS
Department of Materials Science and Engineering, SUNY at Stony Brook, NY 11794, USA.
Samuilov
Vladimir A
VA
Sokolov
Jonathan
J
Rafailovich
Miriam
M
Tinland
Bernard
B
Kim
Jaeseung
J
Chu
Benjamin
B
D000327:Adsorption; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D008863:Microspheres; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide; D013499:Surface Properties
D012822:Silicon Dioxide; D004247:DNA