Large-scale morphological survey of mouse retinal ganglion cells.
Five hundred twenty ganglion cells in an isolated whole-mount preparation of the mouse retina were labeled using the "DiOlistic" method (Gan et al. [2000] Neuron 27:219-225) and were classified according to their morphological properties. Tungsten particles coated with a lipophilic dye (DiI) were propelled into the whole-mount retina using a gene gun. When a dye-coated particle contacted the cell membrane, the entire cell was labeled. The ganglion cells were classified into four groups based on their soma size, dendritic field size, and pattern and level of stratification. Broadly monostratified cells were classified into three groups: RG(A) cells (large soma, large dendritic field), RG(B) cells (small to medium-sized soma, small to medium-sized dendritic field), and RG(C) cells (small to medium-sized size soma, medium-sized to large dendritic field). Bistratified cells were classified as RG(D). This study represents the most complete morphological classification of mouse retinal ganglion cells available to date and provides a foundation for further understanding of the correlation of physiology and morphology and ganglion cell function with genetically manipulated animals.
Sun
Wenzhi
W
Institute of Neuroscience and Shanghai Research Center for Life Sciences, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, People's Republic of China.
Li
Ning
N
He
Shigang
S
D000818:Animals; D002232:Carbocyanines; D048429:Cell Size; D003584:Cytological Techniques; D003712:Dendrites / Q000502:physiology / Q000648:ultrastructure; D004594:Electrophysiology; D051379:Mice; D008810:Mice, Inbred C57BL; D012165:Retinal Ganglion Cells / Q000145:classification / Q000502:physiology* / Q000648:ultrastructure*; D012172:Retinaldehyde / Q000502:physiology; D014414:Tungsten
D002232:Carbocyanines; C072024:carbocyanine dye DiIC12(3); D012172:Retinaldehyde; D014414:Tungsten