[Effects of rhGM-CSF and rhG-CSF on growth of CFU-F derived from acute leukemia (AL) patient bone marrow in vitro].
By using the cultural method of CFU-F derived from bone marrow in the patient with acute leukemia in vitro, we dissected the effects of rhGM-CSF and rhG-CSF on growth of CFU-F derived from AL patient bone marrow. The results showed that rhGM-CSF and rhG-CSF enhanced the number of colonies of CFU-F and its proliferation activity. Both factors had a synergy on the above effects. The effects of rhGM-CSF on CFU-F were significantly greater than that of rhG-CSF. The data indicate that rhGM-CSF and rhG-CSF might regulate hemopoiesis more effectively by stimulating the growth of fibroblast and improving bone marrow microenvironment.
Xu
Y X
YX
Department of Haematological Research, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
Lu
H B
HB
Zhang
G S
GS
D000293:Adolescent; D000328:Adult; D001854:Bone Marrow Cells / Q000473:pathology; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology; D011994:Recombinant Proteins / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor