Status of NAT screening for HCV, HIV and HBV: experience in Japan.
The first nationwide nucleic acid amplification testing (NAT) for hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type 1 (HIV-1) of voluntarily donated blood after serological pre-screening and before release of cellular components and plasma for fractionation was implemented by the Japanese Red Cross Blood Transfusion Services. The NAT screening assay using multiplex reagent is time-saving, cost effective, and labour-saving procedure for all blood and blood products including short-shelf life platelets. During the 50-mini-pool NAT screening of serologically negative donations (February 1, 2001-April 30, 2001), we were able to screen out 112 HBV-positive, 25 HCV-positive, and 4 HIV-1 positive units from blood and blood components.
Tomono
T
T
JRC Headquarter, Blood Services Department. tomono@pfc.jrc.or.jp
Murokawa
H
H
Minegishi
K
K
Yamanaka
R
R
Lizuka
H Y
HY
Miyamoto
M
M
Satoh
S
S
Nakahira
S
S
Murozuka
T
T
Emura
H
H
Doi
Y
Y
Mine
H
H
Yokoyama
S
S
Ohnuma
H
H
Tanaka
T
T
Yoshikawa
A
A
Nishioka
K
K
D001769:Blood / Q000821:virology*; D001782:Blood Donors; D001803:Blood Transfusion; D004279:DNA, Viral; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification; D006518:Hepatitis Viruses / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007564:Japan; D008403:Mass Screening; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis; D012011:Red Cross; D014766:Viremia
D004279:DNA, Viral; D012367:RNA, Viral