title: Amyloid-beta promotes calcium influx and neurodegeneration via stimulation of L voltage-sensitive calcium channels rather than NMDA channels in cultured neurons.
abstract: Exposure of cultured neurons and neuronal cells to aggregated amyloid-beta (Abeta) induces multiple neurodegenerative events including accumulation of cytosolic calcium, generation of reactive oxygen species, abnormal levels of phosphorylation of the microtubule-associated protein tau, and apoptosis. Prevention of accumulation of calcium within the cytosol also prevents all other events, suggesting that calcium accumulation is an early and pivotal event in Abeta neurotoxicity. Calcium influx has been suggested to occur via L voltage-sensitive calcium channels or NMDA channels. Calcium influx into differentiated human neuroblastoma cells has been previously attributed to the L voltage-sensitive calcium channel, but the contribution of the NMDA channel was not examined. In the present study, treatment of these cells with MK-801, an antagonist of NMDA channels, failed to attenuate Abeta-induced calcium influx or neurodegeneration, while nimopridine, an antagonist of the L voltage-sensitive calcium channel, blocked Abeta-induced calcium influx. Our findings suggest that NMDA channels do not contribute significantly to Abeta neurotoxicity in these acute cell culture analyses.
authors.affiliation: Center for Cellular Neurobiology and Neurodegeneration Research, University of Massachusetts, Lowell, Lowell, MA 01854, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Ho
authors.forename: Daniela
authors.initials: D
authors.lastname: Ortiz
authors.forename: Thomas B.
authors.initials: TB
authors.lastname: Shea