The Cloning and Construction of a Murine-human Chimeric Antibody with Specificity for HBsAg and the Expression of the Chimeric Heavy Chain in E. coli.
The genes encoding the heavy and light chain variable regions (V(H) and V(K)) of the murine monoclonal antibody with the specificity for hepatitis B virus surface antigen (HBsAg) have been clone by using RT-PCR. They were combined to the Human C(gamma)(3) and C(K) genes respectively to construct the murine human chimeric antibody genes The chimeric heavy chain was expressed in E. coli, and characterized by SDS-PAGE, Western-blot, indirect ELISA Assay to demonstrate the expression product has the HBsAg-binding ability.
Chen
Ya-Hua
YH
Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
Zhou
Ge
G
Wu
Xiang-Fu
XF
Yu
Yong-Jun
YJ