title: Anti-human immunodeficiency virus-type 1 activity of constituents from Juglans mandshurica.
abstract: Three naphthalene glycosides (1-3), four flavonoids (4-7), and two galloyl glycosides (8-9) were isolated from the stem-bark of Juglans mandshurica (Juglandaceae). Their structures were determined by chemical and spectral means, including to 2D-NMR (COSY, HMQC, and HMBC) experiments. Amongst the isolated compounds, taxifolin (4) showed the most potent HIV-induced cytopethic activity against MT-4 cells with complete inhibitory concentration (IC100) value of 25 microg/ml and maximum cytotoxic concentration (CC100) value of above 100 microg/ml. However, naphthalene glycosides (1-3), flavonoids (5-7), and galloyl tannins (8-9) were inactive against anti-HIV-1 activity.
authors.affiliation: Immunomodulator Research Laboratory, KRIBB, Taejon, Korea. bsmin@kribb.re.kr
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
authors.forename: Hyeong Kyu
authors.initials: HK
authors.lastname: Lee
authors.forename: Sang Myung
authors.initials: SM
authors.lastname: Lee
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Ki Hwan
authors.initials: KH
authors.lastname: Bae
authors.forename: Toru
authors.initials: T
authors.lastname: Otake
authors.forename: Norio
authors.initials: N
authors.lastname: Nakamura
authors.forename: Masao
authors.initials: M
authors.lastname: Hattori
mesh_terms: D019380:Anti-HIV Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D007202:Indicators and Reagents; D031324:Juglans / Q000737:chemistry*; D007723:Korea; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D024301:Plant Bark / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D019380:Anti-HIV Agents; D007202:Indicators and Reagents