title: Beneficial effects of Hippophae rhamnoides L. on nicotine induced oxidative stress in rat blood compared with vitamin E.
abstract: The aim of this study was to determine the effects of Hippophae rhamnoides L. extract (HRe-1) and also vitamin E as a positive control on nicotine-induced oxidative stress in rat blood, specifically alterations in erythrocyte malondialdehyde (MDA) level, activities of some erythrocyte antioxidant enzymes, and plasma vitamin E and A levels. The groups were: nicotine (0.5 mg/kg/d, intraperitoneal, i.p.); nicotine+vitamin E (75 mg/kg/d, intragastric, i.g.); nicotine+HRe-1 (1 ml/kg/d, i.g.); and control group (receiving only vehicles). There were 8 rats per group and the supplementation period was 3 weeks. Nicotine-induced increase in erythrocyte MDA level was prevented by both HRe-1 and vitamin E. Nicotine-induced decrease in erythrocyte superoxide dismutase (SOD) activity was prevented by HRe-1, but not vitamin E. HRe-1 increased the erythrocyte glutathione peroxidase (GSH-Px) activity compared with nicotine and the vitamin E groups. Catalase activity was not affected. Vitamin E supplementation increased plasma vitamin E level. Plasma vitamin A level was higher in both vitamin E and HRe-1 supplemented groups compared with nicotine and control groups. The results suggest that HRe-1 extract can be used as a dietary supplement, especially by people who smoke, in order to prevent nicotine-induced oxidative stress.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Halis
authors.initials: H
authors.lastname: Suleyman
authors.forename: Kenan
authors.initials: K
authors.lastname: Gumustekin
authors.forename: Seyithan
authors.initials: S
authors.lastname: Taysi
authors.forename: Sait
authors.initials: S
authors.lastname: Keles
authors.forename: Nuray
authors.initials: N
authors.lastname: Oztasan
authors.forename: Omer
authors.initials: O
authors.lastname: Aktas
authors.forename: Konca
authors.initials: K
authors.lastname: Altinkaynak
authors.forename: Handan
authors.initials: H
authors.lastname: Timur
authors.forename: Fatih
authors.initials: F
authors.lastname: Akcay
authors.forename: Sedat
authors.initials: S
authors.lastname: Akar
authors.forename: Senol
authors.initials: S
authors.lastname: Dane
authors.forename: Mustafa
authors.initials: M
authors.lastname: Gul
mesh_terms: D000818:Animals; D005638:Fruit; D005979:Glutathione Peroxidase / Q000097:blood; D031281:Hippophae; D008315:Malondialdehyde / Q000097:blood; D009538:Nicotine / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D014810:Vitamin E; D008315:Malondialdehyde; D009538:Nicotine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase