title: High Expression in Insect Cells of A Functional Chimeric Antibody with Specificity for HBsAg.
abstract: We have constructed a double-recombinant virus BacHL4.2 containing both the chimeric heavy- and light-chain cDNAs from monoclonal antibody of the HBsAg gene. Both murine-human chimeric antibody heavy- and light-chain were expressed in Sf9 cells infected with a double-recombinant virus BacHL4.2 or co-infected with separate heavy- and light-chain recombinant viruses. In both cases, expressed products were correctly assembled into normal H(2)L(2) immunoglobulin monomers. ELISA and functional immunoblot assay showed that the recombinant chimeric antibody exhibited a specificity for HBsAg similar to that of the parental murine monoclonal antibody OH3.
authors.affiliation: Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Yu
authors.forename: Yu-Lei
authors.initials: YL
authors.lastname: Jiang
authors.forename: Guan-Zhen
authors.initials: GZ
authors.lastname: Yang
authors.forename: Zai-Ping
authors.initials: ZP
authors.lastname: Li
authors.forename: Xiang-Fu
authors.initials: XF
authors.lastname: Wu