Making 'random amplification' predictable in whole genome analysis.
One of the remaining obstacles to large-scale genetic mapping is the lack of an efficient way to genotype hundreds of thousands of genetic markers. Recently Jordan et al. reported that the 'random' sequences amplified by degenerate oligonucleotide primer (DOP)-PCR can be precisely mapped onto the human genome sequence and that it is possible to predict which DNA sequences will be amplified by a particular degenerate primer.
Kwok
Pui-Yan
PY
University of California, San Francisco, 505 Parnassus Ave., Long 1332A, Box 0130, San Francisco, CA 94118, USA. kwok@cvrimail.ucsf.edu
D017931:DNA Primers; D015894:Genome, Human; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D011786:Quality Control; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*
D017931:DNA Primers