Laminin-1 increases motility, path-searching, and process dynamism of rat and mouse Muller glial cells in vitro: implication of relationship between cell behavior and formation of retinal morphology.
Spatial correlation was observed between the localization of laminin-1 at the inner limiting membrane (ILM) and extensive Muller glial process arborization in the same area, as demonstrated by immunolabeling of Muller glial processes and laminin-1 in rat retinae in situ. To test if this spatial correlation is due to a functional relationship, we investigated the impact of laminin-1 on the motility of cultured primary rat and mouse retinal Muller glial cells by statistical analysis of computer-controlled videomicroscopic time-lapse images. We demonstrate that laminin-1 increases motility and path-searching activity of Muller cells in vitro and it also enhances the cells' process formation/withdrawal dynamism. The increase in path-searching activity and cell process dynamism indicates that there is a functional relationship between laminin-1 and Muller glial cells presumably involving signaling towards the cytoskeleton. We hypothesize that laminin-1 is involved in process arborization of Muller cells at the vitread border of the retina resulting in the formation of the functional barrier made up of Muller glial endfeet.
Méhes
Elod
E
Department of Anatomy and Histology, Faculty of Veterinary Science, Szent István University, Budapest, Hungary.
Czirók
András
A
Hegedüs
Balázs
B
Vicsek
Tamás
T
Jancsik
Veronika
V
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018715:Microscopy, Video; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000166:cytology*; D014746:Vimentin / Q000378:metabolism
D007797:Laminin; D014746:Vimentin; C121346:laminin 1