Secreted phosphatase activity induced by dimethyl sulfoxide in Herpetomonas samuelpessoai.
A phosphatase activity of the trypanosomatid parasite Herpetomonas samuelpessoai was characterized using intact living cells. The effects of dimethyl sulfoxide (DMSO) on this activity were investigated. This phosphatase activity (2.53+/-0.01 nmol P(i)/mg protein x min) was linear with cell density and with time for at least 60 min. The optimum pH for the H. samuelpessoai phosphatase lies in the acid range. This phosphatase activity was inhibited by metal chelators and classical phosphatase inhibitors. A robust stimulation of the phosphatase activity was observed when the flagellates were grown in the presence of 4% DMSO, both when intact flagellates and when culture supernatant from those cells were assayed, as observed by biochemical and cytochemical analysis. We also demonstrate that DMSO induced the secretion and/or shedding of this phosphatase to the extracellular medium, with a possible involvement of protein kinase C in this process.
Santos
André L S
AL
Instituto de Microbiologia Prof. Paulo de Góes, CCS, Universidade Federal do Rio de Janeiro, Cidade Universitária, Ilha do Fundão, RJ 21941-590, Rio de Janeiro, Brazil.
Souto-Padrón
Thais
T
Alviano
Celuta S
CS
Lopes
Angela H S C
AH
Soares
Rosangela M A
RM
Meyer-Fernandes
José R
JR
D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005407:Flagella / Q000187:drug effects; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000378:metabolism*; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism; D013644:Tartrates / Q000494:pharmacology; D014351:Trypanosomatina / Q000187:drug effects* / Q000201:enzymology*
D002614:Chelating Agents; D004791:Enzyme Inhibitors; D013644:Tartrates; D004533:Egtazic Acid; D004492:Edetic Acid; D011493:Protein Kinase C; D010744:Phosphoric Monoester Hydrolases; C029768:tartaric acid; D004121:Dimethyl Sulfoxide