title: Detection of epstein-barr virus in nasal T-cell lymphoma.
abstract: The close relationship between Epstein-Barr virus (EBV) and nasal T-cell lymphoma (NTL) has frequently been reported. However, the status of the infection, either lytic or latent, is obscure. This study involved 16 patients with NTL. Phenotypes of lymphoma cells were examined by immunohistochemical staining using CD3, CD4, CD8, CD20 and CD45RO monoclonal antibodies. EBV-encoded small nuclear RNA (EBER)-1 and EBV NotI tandem repeat region were detected by reverse transcription, using a rapid (< or = 60 min) in situ hybridization technique. Tumor cells expressed at least one T-cell marker, such as CD3, CD4, CD8 and CD45RO. CD20 was not detected in any of the cases. EBER-1 was identified in all cases; no Notl tandem DNA repeat was demonstrated. All cases demonstrated a T-cell phenotype. These data suggest that NTL is associated with EBV infection in the latent phase.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Makoshi
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Takahashi
authors.forename: Toru
authors.initials: T
authors.lastname: Kameya
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yao
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sato
authors.forename: Meijin
authors.initials: M
authors.lastname: Nakayama
authors.forename: Benio
authors.initials: B
authors.lastname: Tsuchiya
authors.forename: Makito
authors.initials: M
authors.lastname: Okamoto
mesh_terms: D015703:Antigens, CD / Q000032:analysis / Q000235:genetics; D020031:Epstein-Barr Virus Infections / Q000235:genetics* / Q000382:microbiology / Q000473:pathology*; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D017403:In Situ Hybridization; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000382:microbiology / Q000473:pathology*; D009296:Nasal Cavity / Q000382:microbiology* / Q000473:pathology*; D009669:Nose Neoplasms / Q000235:genetics* / Q000382:microbiology / Q000473:pathology*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020080:Tandem Repeat Sequences / Q000235:genetics
chemical_list: D015703:Antigens, CD; C099537:Epstein-Barr virus encoded RNA 1; D012367:RNA, Viral