[The effects of a novel aminosteroid on WEHI-3B leukemia cells].
OBJECTIVE
To evaluate the effect of a novel aminosteroid(KH) on WEHI-3B cells.


METHODS
The effects and mechanism of KH on proliferation, differentiation and apoptosis of WEHI-3B cells in vitro were studied by semi-solid colony culture, MTT assay, morphologic examination, NBT reduction test, NSE assay, DNA fragmentation in gel electrophoresis and RT-PCR of c-myc oncogene.


RESULTS
The growth of leukemic colony was inhibited by KH(10(-8)-10(-4) mol.L-1) after treatment of KH for 7 days. The percentages of NBT and NSE positive cells were increased from 40.38% to 71.17% and 48.25% to 79.25%, respectively as well as the typical ladders of DNA fragments in gel electrophoresis were also observed after WEHI-3B cells were treated with KH(10(-8)-10(-4) mol.L-1) for 5 days. The c-myc mRNA expression of WEHI-3B cells was decreased by 58.7% after WEHI-3B cells were treated with KH(10(-8) mol.L-1) for 5 days.


CONCLUSIONS
KH might suppress the proliferation of WEHI-3B cells, induce the differentiation of WEHI-3B cells into macrophage-like cells and facilitate the cell apoptosis. There is relationship between the above effects and c-myc mRNA expression of WEHI-3B cells.
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Research Laboratory of Blood Physiology, Department of Physiology, Hunan Medical University, Changsha 410078.
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Jiang
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D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D015479:Leukemia, Myelomonocytic, Acute / Q000473:pathology*; D051379:Mice; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013256:Steroids / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D013256:Steroids