title: An improved western blotting technique effectively reduces background.
abstract: We have developed a simple method to dramatically improve Western blotting by use of water washing as well as altered buffer conditions. Our improved protocol reduces the cost and labor of Western blotting by shortening the washing time and uses less buffer and other reagents. This procedure does not significantly change existing protocols of Western blotting analysis; it simply contains a few modifications to the washing step and buffer conditions. Our improved technique may therefore have broad applications in various experimental conditions.
authors.affiliation: Division of Pulmonary, Allergy, Critical Care and Occupational Medicine, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN 46202-2879, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Peter G
authors.initials: PG
authors.lastname: Stockley
authors.forename: William J
authors.initials: WJ
authors.lastname: Martin
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002021:Buffers; D002460:Cell Line; D006801:Humans; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D019950:Mitogen-Activated Protein Kinase 1 / Q000032:analysis; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000032:analysis; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000032:analysis; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D051381:Rats; D019096:Vitronectin / Q000032:analysis; D014867:Water
chemical_list: D002021:Buffers; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D011506:Proteins; D019096:Vitronectin; D014867:Water; D019853:O(6)-Methylguanine-DNA Methyltransferase; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases