Expression of Human Low-affinity Nerve Growth Factor Receptor in Mammanlian Neural Cells and its Induction of Apoptosis.
Human low-affinity nerve growth factor receptor (p75NGFR) was amplified from human fetal brain by RT-PCR and ligated into the retrovirus expression vector pXT-1. The cloned pXT-1/p75NGFR was packaged by PA37 cell line and the collected pseudovirus was used to infect neural cell line R2. The expression of p75NGFR on R2 cells at the RNA transcriptional and protein translation levels were demonstrated by Northern bloting and FITC-labeled immunological analysis. Further studies indicated that p75NGFR could induce apoptosis of neural cells in culture medium deprived of serum. Such cell death could be prevented by inhibitors of macromolecular syntheses such as actinomycin D and cycloheximidine.
Gu
Ji-Jie
JJ
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College and Chinese Academy of Medical Science, Beijing 100005,China.
Ming
Hong
H
Huang
Bing-Ren
BR
Cai
Liang-Wan
LW