title: Novel characterisation of the gene encoding conglutinin reveals that previously characterised promoter corresponds to the CL-43 promoter.
abstract: Conglutinin, a collectin found in bovine serum, is an opsonin that binds to glycoconjugates on the surface of microorganisms or on deposited iC3b, and acts in concert with phagocytes to establish a first-line of immune defense. We have isolated a genomic conglutinin phage clone and found that the 5'-flanking region shows 95.8% identity with the sequence previously published, which on the other hand shows 99.7% identity with the CL-43 promoter. We conclude that the previously published promoter corresponds to the CL-43 promoter and that the functional studies performed on it apply to transcription of CL-43. Comparison of potential cis-regulatory elements in relation to the functional studies indicates that the two genes are regulated by different mechanisms.
authors.affiliation: Department of Immunology and Microbiology, University of Southern Denmark, Odense, Winsloewparken 21.1, DK-5000 Odense C, Denmark.
authors.forename: Soren
authors.initials: S
authors.lastname: Hansen
authors.forename: Vivi
authors.initials: V
authors.lastname: Moeller
authors.forename: Dorte
authors.initials: D
authors.lastname: Holm
authors.forename: Lars
authors.initials: L
authors.lastname: Vitved
authors.forename: Christian
authors.initials: C
authors.lastname: Bendixen
authors.forename: Karsten
authors.initials: K
authors.lastname: Skjodt
authors.forename: Uffe
authors.initials: U
authors.lastname: Holmskov
mesh_terms: D001483:Base Sequence; D037222:Collectins / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012712:Serum Globulins / Q000235:genetics*
chemical_list: D037222:Collectins; D012712:Serum Globulins; C024014:conglutinin; C086954:collectin-43 protein, bovine