title: Analysis of gene expression in Egr-1 transfected human fibrosarcoma cells.
abstract: OBJECTIVE
  It has been shown previously that transcription factor Egr-1 functions as a tumor suppressor gene. This study was conducted to investigate the possible mechanisms responsible for this function.
  
  
  METHODS
  A cDNA microarray technique was applied to study differential gene expression between Egr-1-transfected cells and control-transfected cells.
  
  
  RESULTS
  A number of genes differentially expressed were identified. Among 5,184 genes examined by cDNA microarray technique, 53 were up-regulated in Egr-1 transfected cells, and 276 were down-regulated in Egr-1 transfected cells by at least two times or more; 13 genes were independently confirmed by reverse transcription polymerase chain reaction (RT-PCR). Of these 13 genes, 11 were in agreement with cDNA microarrays in terms of gene expression levels.
  
  
  CONCLUSIONS
  Egr-1 regulated almost every category of genes according to gene ontology system, and repressed more genes than it induced. The identification and further characterization of genes regulated by Egr-1 should provide valuable information to understand Egr-1 tumor suppressor action and the role of Egr-1 as a transcription factor in general.
authors.affiliation: Division of Research, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
authors.forename: Ya-Guang
authors.initials: YG
authors.lastname: Liu
authors.forename: Jacob
authors.initials: J
authors.lastname: Gano
authors.forename: Ruochun
authors.initials: R
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
authors.forename: Shen-Ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Ruo-Pan
authors.initials: RP
authors.lastname: Huang
mesh_terms: D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D015536:Down-Regulation; D051766:Early Growth Response Protein 1; D005354:Fibrosarcoma / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D017874:Immediate-Early Proteins; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D017874:Immediate-Early Proteins; D014157:Transcription Factors