[Inhibitory effects of 8-methoxypsoralen combining with all trans retinoic acid on oral squamous cancer cell lines].
To study the inhibitory effects of 8-methoxypsoralen (8-MOP) combining with all trans retinoic acid (RA) on tongue cancer cell line Tca8113 and gingiva cancer cell line Ca9-22, MTT assay (tetrazolium-based colormetric assay) was applied and the changes in morphology were observed with the inverted microscope. The results showed that with 30% growth inhibition of the cell lines, the Combination Indexes (CI30) of the two drugs were no more than 0.82, and with 50% growth inhibition, the Combination Index (CI50) were from 0.9 to 0.95, which indicated synergic combination effects of the two drugs against the two oral squamous cancer cell lines. Apoptosis-body-like structures were observed in the cells treated with RA, 8-MOP, or the combination of RA with 8-MOP. Whether combination of the two drugs at appropriate concentrations has clinical value needs to be investigated.
Yan
X
X
College of Stomatology, Fourth Military Medical University.
Wu
J
J
Chen
J
J
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D008730:Methoxsalen / Q000494:pharmacology*; D014062:Tongue Neoplasms / Q000473:pathology*; D014212:Tretinoin / Q000494:pharmacology*
D014212:Tretinoin; D008730:Methoxsalen