Antigenic relationships between six genotypes of the small hydrophobic protein gene of mumps virus.
Six different genotypes of mumps virus, A, C, D, G, H and I, genotyped on the basis of the small hydrophobic protein gene sequence, were subjected to antigenic comparison. Monoclonal antibodies directed against the haemagglutinin-neuraminidase protein of the SBL-1 strain of genotype A were used in immunofluorescence tests with different mumps virus strains. In addition, the six virus genotypes were compared by cross-neutralization tests with human post-vaccination sera after vaccination with the Jeryl Lynn (JL) strain of mumps virus and with rabbit hyperimmune sera directed against the A or D genotypes of mumps virus. Genotypes C, D, G, H and I could not be antigenically separated. In contrast, three different virus strains of genotype A, SBL-1, JL and Kilham, were distinct and were found to represent three different serotypes within the A genotype of mumps virus. Vaccination of Swedish children with the JL strain of mumps virus resulted in clearly lower neutralization titres against the SBL-1 strain, which is endemic in Sweden, compared to the homologous vaccine titres.
Örvell
Claes
C
Huddinge University Hospital, Department of Clinical Virology, Karolinska Institutet, SE-141 86 Stockholm, Sweden1.
Tecle
Tesfaldet
T
Huddinge University Hospital, Department of Clinical Virology, Karolinska Institutet, SE-141 86 Stockholm, Sweden1.
Johansson
Bo
B
Huddinge University Hospital, Department of Clinical Virology, Karolinska Institutet, SE-141 86 Stockholm, Sweden1.
Saito
Hiroyuki
H
Department of Microbiology, Akita Prefectural Institute of Public Health, Akita 010-0874, Japan2.
Samuelson
Agneta
A
Huddinge University Hospital, Department of Clinical Virology, Karolinska Institutet, SE-141 86 Stockholm, Sweden1.
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009109:Mumps virus / Q000235:genetics* / Q000276:immunology; D009500:Neutralization Tests; D010802:Phylogeny; D011817:Rabbits; D017422:Sequence Analysis, DNA
D000914:Antibodies, Viral; D000956:Antigens, Viral