title: Purification and characterization of two forms of methanol dehydrogenases from a marine methylotroph.
abstract: Two methanol dehydrogenases (MDHs), MDH1 and MDH2, were purified from a marine methylotroph, Methylophaga sp. strain 1. Both enzymes had very similar properties, including the same native molecular weight, sizes of subunits and substrate specificity. The N-terminal amino acid sequence of the alpha-subunit of MDH2 differed from that of MDH1 by having a histidine residue at a highly conserved glutamate position, but both sequences showed approximately 50% homology to the alpha-subunits of other MDHs. MDH1 had higher specific activity than MDH2 with respect to methanol and ethanol as a substrate. The two enzymes did not appear to be isoforms but that either MDH1 or MDH2 could be a mutant arising from spontaneous mutation.
authors.affiliation: Department of Biological Sciences and Research Center for Proteineous Materials, Chosun University, Gwangju 501-759, Republic of Korea.
authors.forename: Alan Kuei-Chieh
authors.initials: AK
authors.lastname: Chang
authors.forename: Chae Young
authors.initials: CY
authors.lastname: Lim
authors.forename: Si Wouk
authors.initials: SW
authors.lastname: Kim
authors.forename: Ho Jin
authors.initials: HJ
authors.lastname: You
authors.forename: Kyung Soo
authors.initials: KS
authors.lastname: Hahm
authors.forename: Seong Myeong
authors.initials: SM
authors.lastname: Yoon
authors.forename: Jong Kun
authors.initials: JK
authors.lastname: Park
authors.forename: Jung Sup
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D020563:Gammaproteobacteria / Q000201:enzymology*; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D000432:Methanol / Q000378:metabolism; D008969:Molecular Sequence Data; D012623:Seawater / Q000382:microbiology*; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); D000432:Methanol