Construction and Physical Mapping of the 35 cM YAC Contigs for the Region of Xp11.2 - p21.3.
One hundred and fifty-one YACs were selected from three libraries with a series of STS and other DNA probes of the human X chromosome p11.2 - p21.3 region. The YAC contigs, spanning about 35cM in all and generally cover the whole region, were constructed based on the physical analysis of the positive clones. A total of seventy-seven DNA markers of this region were located and ordered on this map with an average 454 kb spacing, in which fifty-three were polymorphic markers with an average 0.6 cM genetic spacing. These results facilitate the search for novel genes and the sequence analysis of this region.
Wei
Yong
Y
Institute of Genetics, Fudan University, Shanghai 200433, China.
Wu
Xue-Jun
XJ
Miao
Wei-Min
WM
Cai
Ti
T
Wu
Xiao-Hui
XH
Kang
Yi-Bin
YB
Chai
Jian-Hua
JH