Anomalously high cooperativity of oligodeoxycytidylic acid for luminescence resonance energy transfer to lanthanide ions.
The luminescence of terbium(III) and europium(III) through luminescence resonance energy transfer from mononucleotides and oligodeoxynucleotides is examined. Among mononucleotides, dGMP gives the strongest luminescence of terbium(III), while dTMP and dCMP yield a luminescence intensity of europium(III) that is larger than the other two cases. In the homodeoxydecamers, decadeoxycytidylic acid (dC10) produces the highest intensity for both metals. The anomalously large cooperativity of dC10 is explained by the easiness of deformation of the helical structure to bind lanthanide ions, and a circular dichroism study supports this explanation.
Lee
Jihee
J
Center for Integrated Molecular Systems, Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang 790-784, Korea.
Park
Minyoung
M
Son
Hyung Su
HS
Lee
Sang Bum
SB
Lee
Hee Cheon
HC
Ku
Ja Kang
JK
Park
Joon Won
JW
D002942:Circular Dichroism; D004735:Energy Transfer; D005063:Europium; D007700:Kinetics; D028581:Lanthanoid Series Elements; D008163:Luminescent Measurements; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000032:analysis* / Q000737:chemistry*; D013725:Terbium
D028581:Lanthanoid Series Elements; D009838:Oligodeoxyribonucleotides; C033539:oligodeoxycytidylic acid; D013725:Terbium; D005063:Europium