title: Activity staining of plasma amine oxidase after polyacrylamide gel electrophoresis and its application to natural inhibitor screening.
abstract: Plasma amine oxidase (plasma AO, EC 1.4.3.6) is a copper-containing AO which converts benzylamine (BZ) to benzaldehyde, generating hydrogen peroxide and ammonia. The peroxidase was used as an ancillary enzyme to couple hydrogen peroxide to 3-amino-9-ethylcarbazole (AEC) to achieve plasma AO activity after electrophoresis on native polyacrylamide gels. It was confirmed that plasma AO is inhibited by semicarbazide but neither by clorgyline nor by deprenyl. We also used plasma AO activity staining for the screening of natural inhibitors. This fast and sensitive method can be used in the process of plasma AO purification, characterization, and inhibitor screening.
authors.affiliation: Graduate Institute of Pharmacognosy Science, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Mei-Hsien
authors.initials: MH
authors.lastname: Lee
authors.forename: Mao-Te
authors.initials: MT
authors.lastname: Chuang
authors.forename: Wen-Chi
authors.initials: WC
authors.lastname: Hou
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors* / Q000097:blood* / Q000302:isolation & purification; D000818:Animals; D002417:Cattle; D004353:Drug Evaluation, Preclinical; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006861:Hydrogen Peroxide; D066298:In Vitro Techniques; D012663:Semicarbazides / Q000494:pharmacology; D013194:Staining and Labeling
chemical_list: D004791:Enzyme Inhibitors; D012663:Semicarbazides; C010059:carbamylhydrazine; D006861:Hydrogen Peroxide; D006631:Amine Oxidase (Copper-Containing)