Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p.
The cytoplasmic tail of Kre6p, a Golgi membrane protein involved in cell wall synthesis, interacts with the actin patch assembly components Las17p and Sla1p in a two-hybrid assay, and Kre6p co-immunoprecipitates with Las17p. Kre6p showed extensive co-localization with Och1p-containing cis-Golgi vesicles. The correct localization of Kre6p requires its cytoplasmic tail, Las17p, Sla1p and Vrp1p, suggesting that the cytoplasmic tail of Kre6p acts as a receptor, linking this cis-Golgi protein to Las17p and Sla1p. The actin patch assembly mutants las17 delta, sla1delta and vrp1 delta showed elevated levels of cell wall beta-1,6-glucan, and mutant cells were capable of only a limited number of cell divisions compared to wild-type. EM image analysis and beta-1,6-glucan localization indicated abnormal wall proliferation in the mother cells of these mutants. The pattern of cell wall hypertrophy indicates a failure to restrict cell wall growth to the bud.
Li
Huijuan
H
Department of Biology, McGill University, Montreal H3A 1B1, Canada.
Pagé
Nicolas
N
Bussey
Howard
H
D000199:Actins / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry*; D002473:Cell Wall / Q000378:metabolism*; D003593:Cytoplasm / Q000737:chemistry; D003598:Cytoskeletal Proteins; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry*; D006056:Golgi Apparatus / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D020798:Two-Hybrid System Techniques; D051316:Wiskott-Aldrich Syndrome Protein
D000199:Actins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D005656:Fungal Proteins; C081933:KRE6 protein, S cerevisiae; C480361:LAS17 protein, S cerevisiae; D008565:Membrane Proteins; C082265:SLA1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D051316:Wiskott-Aldrich Syndrome Protein