[Allelic polymorphism of HLA-DQA1 locus in Han nationality in Hunan province].
OBJECTIVE
To explore the genetic polymorphism of HLA-DQA1 locus in Han nationality in Hunan province.


METHODS
Sixty samples randomly selected from healthy individuals of Han nationality in Hunan province were typed for HLA-DQA1 by PCR/SSP in combination with silver-staining PCR/SSCP.


RESULTS
Ten types of HLA-DQA1 alleles were found in all 60 samples, HLA-DQA1*0302, *0501, *0102 were the common alleles with gene frequencies of 0.2254, 0.2254, and 0.2041 respectively. Twenty-six kinds of genotypes were found, and the genotype distribution fitted the Hardy-Weinberg equilibrium test (P > 0.05). Five samples which had ambiguity in their PCR/SSP typing were further clarified with PCR/SSCP analysis.


CONCLUSIONS
Normal gene frequency values of HLA-DQA1 alleles in Han nationality in Hunan province are provided; PCR/SSCP can be used to eliminate the ambiguity which might exist in PCR/SSP typing for HLA-DQA1 locus.
Tian
W
W
Immunology Research Laboratory, Hunan Medical University, Changsha 410078.
Li
L X
LX
Guo
S S
SS
D000483:Alleles; D044466:Asian People; D002681:China; D005787:Gene Frequency; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D006801:Humans; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011897:Random Allocation
D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen