Gluconacetobacter diazotrophicus, a sugar cane endosymbiont, produces a bacteriocin against Xanthomonas albilineans, a sugar cane pathogen.
Gluconacetobacter diazotrophicus in liquid culture secretes proteins into the medium. Both medium containing Gluconacetobacter protein and a solution of this protein after acetone precipitation appeared to inhibit the growth of Xanthomonas albilineans in solid culture. This apparent inhibition of bacterial growth has, in fact, been revealed to be lysis of bacterial cells, as demonstrated by transmission electron microscopy. Fractionation of the Gluconacetobacter protein mixture in size-exclusion chromatography reveals a main fraction with lysozyme-like activity which produces lysis of both living bacteria and isolated cell walls.
Piñón
Dolores
D
Casas
Mario
M
Blanch
María
M
Fontaniella
Blanca
B
Blanco
Yolanda
Y
Vicente
Carlos
C
Solas
María-Teresa
MT
Legaz
María-Estrella
ME
D016947:Acetobacteraceae / Q000378:metabolism* / Q000502:physiology; D001430:Bacteriocins / Q000096:biosynthesis* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology; D006109:Poaceae / Q000382:microbiology*; D013559:Symbiosis; D014974:Xanthomonas / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
D001430:Bacteriocins