title: [Determination of vanillymandelic acid in urine by high performance liquid chromatography].
abstract: OBJECTIVE
  To develop a high performance liquid chromatography(HPLC) for determining vanillylmandelic acid(VMA) in urine.
  
  
  METHODS
  The analytical column was packed with Nova-Pak C18. Flow rate was 0.9 ml.min-1. The mobile phase was 40 mmol.L-1 NaH2PO4 buffer. The VMA of 30 healthy people in 24 hour urine were tested by the internal standard method and with electrochemical detector.
  
  
  RESULTS
  The linear range was from 5 mumol.L-1 to 200 mumol.L-1. The mean analytical recovery rate was 99.1%. The mean within-day coefficient of variation(CV) was 5.8%, and the mean day-to-day CV was 7.7%. The detection limit was 0.28 mumol.L-1. The VMA of 24 hour urine was 18.13 +/- 6.17 mumol in healthy people.
  
  
  CONCLUSIONS
  The method is suitable for routine determination of VMA in urine.
authors.affiliation: Department of Medical Laboratory, Xiangya Hospital, Hunan Medical University, Changsha 410008.
authors.forename: L M
authors.initials: LM
authors.lastname: Zheng
authors.forename: Y
authors.initials: Y
authors.lastname: Tang
authors.forename: T
authors.initials: T
authors.lastname: Shen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D014642:Vanilmandelic Acid / Q000652:urine*
chemical_list: D014642:Vanilmandelic Acid