Influence of Lipid Composition on the Interaction of Apolipoprotein Model Peptides with Liposomes.
Two amphiphilic peptides, Ampl and Amp2, were synthesized according to the sequence of the lipid-binding domain in apoliporotein. Amp2 has a Val residue substituted for the Lys at the 4th position of Ampl. Interaction between Ampl / Amp2 and liposomes with different lipid compositions were compared by studying the blue shifts of the intrinsic fluorescence emission maxima, the peptide-induced lipsome leakage and the quenching of tryptophan fluorescence by acrylamide. The influence of temperature on interactions was studied as well. Ampo1 interacted stronger with acidic lipids while Amp2 interacted stronger with zwitterionic lipids. The interaction was reinforced with the increasing extent of lipid unsaturation as well as with the increase of temperature. The lipid unsaturation had amore prominent effect.
Wang
Qing-Da
QD
State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
Cui
Da-Fu
DF
Lin
Qi-Shui
QS