Inclusion counter: a public domain image analysis system for measurement of Chlamydiaceae growth in vitro.
The traditional method of measuring chlamydial growth in vitro, counting Chlamydiaceae inclusions by eye, is time-consuming and error prone. This paper describes a novel automated image analysis system suitable for high-throughput screening of novel anti-Chlamydiaceae compounds. The software, Inclusion Counter v3.0, is freely available in the public domain (http://www.image-analysis.co.uk).
Brown
Jeremy K
JK
Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK. jeremy.brown2@ed.ac.uk
Howie
Sarah E M
SE
Entrican
Gary
G
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002460:Cell Line; D002693:Chlamydiaceae / Q000187:drug effects / Q000254:growth & development* / Q000648:ultrastructure; D023501:Chlamydophila / Q000187:drug effects; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D002479:Inclusion Bodies / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests / Q000379:methods; D012394:Rosaniline Dyes; D012984:Software; D013194:Staining and Labeling / Q000379:methods
D000900:Anti-Bacterial Agents; D012394:Rosaniline Dyes; C006898:carbol-fuchsin solution