title: Palgin sensitizes the adriamycin-induced apoptosis via the enhancement of Fas/Fas ligand expression.
abstract: This study was designed to evaluate the synergistic cytotoxicity of herb prescription, Palgin, in adriamycin-treated cancer cells. The combination of Palgin and adriamycin synergistically augmented the cytotoxicity of Chang and HL-60 cells, but not in Hep3B and Alexander cells. The cytotoxicity of two drugs was revealed as apoptosis characterized by nuclear fragmentation. The apoptotic cell death was accompanied by the activation of caspase-3 as well as cleavage of poly(ADP) ribose polymerase (PARP) in Chang cells. Interestingly, a synergistic increase in apoptosis by the combination of two drugs was accompanied by the enhancement of Fas and Fas ligand (FasL) expression in Chang cells. Taken together, the combination of Palgin and adriamycin significantly augmented the apoptotic cytotoxicity of Fas-positive cells, such as Chang and HL-60 cells, via activation of caspase signaling pathway. This notion will provide a new trial to treat cancer patients in clinical fields as a complementary treatment of Western and Oriental medicine.
authors.affiliation: Professional Graduate School of Oriental Medicine, Wonkwang University, 344-2 Shinyong-dong, Iksan, Jeonbuk 570-749, South Korea.
authors.forename: Yun-Sook
authors.initials: YS
authors.lastname: Lim
authors.forename: Hong-Seob
authors.initials: HS
authors.lastname: So
authors.forename: Myung-Sunny
authors.initials: MS
authors.lastname: Kim
authors.forename: Gu
authors.initials: G
authors.lastname: Moon
authors.forename: Jin-Hee
authors.initials: JH
authors.lastname: Won
authors.forename: Seung-Wha
authors.initials: SW
authors.lastname: Baek
authors.forename: Sun-Rock
authors.initials: SR
authors.lastname: Moon
authors.forename: Sei-Hoon
authors.initials: SH
authors.lastname: Yang
authors.forename: Byoung-Ju
authors.initials: BJ
authors.lastname: Kim
authors.forename: Chang-Bo
authors.initials: CB
authors.lastname: Ko
authors.forename: Raekil
authors.initials: R
authors.lastname: Park
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology*; D004789:Enzyme Activation; D053222:Fas Ligand Protein; D018922:HL-60 Cells; D006801:Humans; D006868:Hydrolysis; D008562:Membrane Glycoproteins / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D002843:Chromatin; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D010936:Plant Extracts; D019014:fas Receptor; C467627:palgin; D004317:Doxorubicin; D011065:Poly(ADP-ribose) Polymerases; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases