title: Immunolocalization of PICK1 in the ascending auditory pathways of the adult rat.
abstract: Protein that interacts with C-kinase alpha (PICK1) is a PDZ domain protein that interacts with many binding partners in the central nervous system (CNS), including activated protein kinase Calpha and subunits of the AMPA subtype of glutamate receptor. Almost nothing is known about the anatomic distribution of PICK1 in the intact adult CNS. By using PICK1 antisera and peroxidase immunocytochemistry, we report on the distribution of PICK1 in the ascending pathways of the central auditory system of the adult rat. PICK1-immunoreactivity (ir) was observed in many component nuclei of the central auditory system, including the dorsal cochlear nucleus, anteroventral cochlear nucleus, posteroventral cochlear nucleus, some divisions of the superior olivary complex, inferior colliculus, medial geniculate body, and primary auditory cortex. The general staining pattern for PICK1-immunoreactivity was somatodendritic with scattered puncta in neuropil and somatodendritic regions. The distribution of PICK1 partially overlaps with PKCalpha and glutamate receptor subunits such as GluR2. These data suggest that PICK1 may function in the regulation of PKCalpha and GluR2 localization in components of the rat auditory system, which may be a fundamental mechanism of synaptic transmission and/or plasticity. J. Comp. Neurol.
authors.affiliation: Neuroscience Program, Tulane Medical School, New Orleans, Louisiana 70112, USA.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: McInvale
authors.forename: Jeff
authors.initials: J
authors.lastname: Staudinger
authors.forename: Richard E
authors.initials: RE
authors.lastname: Harlan
authors.forename: Meredith M
authors.initials: MM
authors.lastname: Garcia
mesh_terms: D000818:Animals; D001303:Auditory Cortex / Q000033:anatomy & histology / Q000502:physiology; D001306:Auditory Pathways / Q000033:anatomy & histology* / Q000502:physiology*; D002352:Carrier Proteins / Q000378:metabolism*; D017626:Cochlear Nucleus / Q000033:anatomy & histology / Q000502:physiology; D003598:Cytoskeletal Proteins; D005829:Geniculate Bodies / Q000033:anatomy & histology / Q000502:physiology; D007150:Immunohistochemistry; D007245:Inferior Colliculi / Q000033:anatomy & histology / Q000502:physiology; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism*; D009847:Olivary Nucleus / Q000033:anatomy & histology / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D009687:Nuclear Proteins; C498298:PICK1 protein, rat