Hydrosalpinx fluid diminishes endometrial cell HOXA10 expression.
OBJECTIVE
To determine the effect of hydrosalpinx fluid on the expression of HOXA10, an essential regulator of endometrial receptivity.


METHODS
In vitro study.


METHODS
Academic medical center.


METHODS
Patients with unilateral or bilateral hydrosalpinx.


METHODS
Hydrosalpinx fluid was aspirated from 10 patients at laparoscopy. The fluid was serially diluted in minimum essential medium. Ishikawa cells (an endometrial adenocarcinoma cell line, representative of endometrial epithelium) were incubated with this fluid at concentrations of 10% and 50% for 48 hours. Cells were also incubated in undiluted minimum essential medium (MEM) and in 10% serum as controls. After incubation, the cells were lysed in Trizol, and total RNA was extracted and analyzed by Northern blot using a 32P-labeled HOXA10 riboprobe. A 32P-labeled G3PDH probe was used as a control for loading.


METHODS
HOXA10 mRNA expression.


RESULTS
HOXA10 mRNA expression in endometrial cells decreased with increasing concentrations of hydrosalpinx fluid. Densitometric analysis of the northern blot revealed that HOXA10 mRNA expression was different from control at both concentrations (P<.007).


CONCLUSIONS
HOXA10 is necessary for implantation in the murine model. HOXA10 expression is diminished by hydrosalpinx fluid. This effect on HOXA10 is a potential molecular mechanism by which implantation rates are diminished in women with hydrosalpinges.
Daftary
Gaurang S
GS
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Taylor
Hugh S
HS
D015152:Blotting, Northern; D001826:Body Fluids / Q000502:physiology; D004717:Endometrium / Q000502:physiology / Q000503:physiopathology*; D005184:Fallopian Tube Diseases / Q000235:genetics / Q000503:physiopathology*; D005260:Female; D005801:Genes, Homeobox; D006801:Humans; D007247:Infertility, Female / Q000209:etiology; D012488:Salpingitis / Q000235:genetics / Q000503:physiopathology*