Development of a frozen cell array as a high-throughput approach for cell-based analysis.
Recent advances in molecular biology, human genetics, and functional genomics tremendously increase the number of molecular targets available for potential therapeutic and diagnostic use. To complement DNA array data, cost-efficient high-throughput technologies providing reliable information at the protein level need to be developed. Here we describe the generation of a frozen cell array that required the use of single cell suspensions and could serve various applications such as the analysis of specific antibody or ligand binding to a large panel of different cell types. As an example, binding of an anti-human epithelial cell adhesion molecule monoclonal antibody to 24 different cell lines has been analyzed using the cell array and compared to the data generated by fluorescence-activated cell sorting. The reliability and flexibility of our frozen cell array technology is compatible with the needs of high-throughput screening for drug discovery and target validation.
Stephan
Jean Philippe
JP
Department of Assay and Automation Technology, South San Francisco, California 94080, USA. stephanj@gene.com
Schanz
Silvia
S
Wong
Anne
A
Schow
Peter
P
Wong
Wai Lee T
WL
D000906:Antibodies / Q000032:analysis; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules / Q000032:analysis; D002460:Cell Line; D015925:Cryopreservation; D003584:Cytological Techniques; D000071858:Epithelial Cell Adhesion Molecule; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D012680:Sensitivity and Specificity
D000906:Antibodies; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule