Molecular identification of Trichophyton rubrum isolate from a dog by chitin synthase 1 (CHS1) gene analysis.
A nonsporulating isolate from a dog with dermatophytosis was identified as Trichophyton rubrum by molecular analysis. The nucleotide sequence analysis of the chitin synthase 1 (CHS1) gene from the isolate indicated more than 99% sequence similarity with other human and canine isolates of T rubrum. The molecular typing suggested that isolates of T. rubrum from human and canine sources were genetically identical.
Kano
R
R
Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan. kano@brs.nihon-u.ac.jp
Hirai
A
A
Yoshiike
M
M
Nagata
M
M
Nakamura
Y
Y
Watanabe
S
S
Hasegawa
A
A
D000818:Animals; D002687:Chitin Synthase / Q000235:genetics* / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis; D003881:Dermatomycoses / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D016533:Mycological Typing Techniques; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014249:Trichophyton / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D004271:DNA, Fungal; D002687:Chitin Synthase