DNA-induced partial unfolding of prion protein leads to its polymerisation to amyloid.
The full-length mouse recombinant prion protein (23-231 amino acid residues) contains all of its structural elements viz. three alpha-helices and a short two-stranded antiparallel beta-sheet in its C-terminal fragment comprising 121-231 amino acid residues. The incubated mixture of this prion protein fragment and nucleic acid results in the formation of amyloid fibres evidenced from electron microscopy, birefringence and fluorescence of the fibre bound Congo Red and Thioflavin T dyes, respectively. The secondary structure of the amyloid formed in nucleic acid solution is similar to the in vivo isolated prion protein 27-30 amyloid but unlike in it, a hydrophobic milieu is absent in the 121-231 amyloid. Thermal denaturation study demonstrates a partial unfolding of the protein fragment in nucleic acid solution. We propose that nucleic acid catalyses unfolding of prion protein helix 1 followed by a nucleation-dependent polymerisation of the protein to amyloid.
Nandi
P K
PK
Institut National de la Recherche Agronomique, Pathologie Infectieuse et Immunologie, 37380 Nouzilly, France. nandi@tours.inra.fr
Leclerc
E
E
Nicole
J-C
JC
Takahashi
M
M
D000682:Amyloid / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D000818:Animals; D052160:Benzothiazoles; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D001718:Birefringence; D002942:Circular Dichroism; D003224:Congo Red / Q000378:metabolism; D004247:DNA / Q000494:pharmacology*; D005453:Fluorescence; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D008854:Microscopy, Electron; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D011328:Prions / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D017433:Protein Structure, Secondary / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013844:Thiazoles / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism
D000682:Amyloid; D000817:Anilino Naphthalenesulfonates; D052160:Benzothiazoles; D001704:Biopolymers; D010446:Peptide Fragments; D011328:Prions; D013844:Thiazoles; C100569:prion protein (121-231); C009462:thioflavin T; D003224:Congo Red; C027132:1-anilino-8-naphthalenesulfonate; D014364:Tryptophan; D004247:DNA