title: Expression and activity of matrix metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ.
abstract: Tooth germ development is associated with morphological and biochemical changes of the dental papilla and enamel organ. Enzymes with gelatinolytic activities were studied by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzymography in tooth germ of newborn to 15-day-old rats. Three major bands with gelatinolytic activity were detected at all periods and characterized as the latent and active forms of MMP-2 using their molecular weight and activity dependent on Zn++ and Ca++ ions as criteria. Expression and activity of MMP-2 increased progressively from 0 to 15 days after birth. Mechanical separation of the tooth germ from 10-day-old rats showed that the gelatinolytic activity was localized mainly in the dental papilla and not the dental organ. These data indicate that the expression and activity of MMP-2 varies during the development and maturation of rat first molar tooth germ.
authors.affiliation: Faculty of Dentistry of Piracicaba, UNICAMP, SP, Brazil.
authors.forename: Paola
authors.initials: P
authors.lastname: Cotrim
authors.forename: Cleverton Roberto
authors.initials: CR
authors.lastname: de Andrade
authors.forename: Sergio
authors.initials: S
authors.lastname: Line
authors.forename: Oslei Paes
authors.initials: OP
authors.lastname: de Almeida
authors.forename: Ricardo D
authors.initials: RD
authors.lastname: Coletta
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D003720:Densitometry; D003771:Dental Papilla / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004658:Enamel Organ / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors; D008963:Molar / Q000201:enzymology*; D008970:Molecular Weight; D009805:Odontogenesis / Q000235:genetics / Q000502:physiology; D010618:Phenanthrolines / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D014083:Tooth Germ / Q000201:enzymology*; D015032:Zinc / Q000378:metabolism
chemical_list: D015853:Cysteine Proteinase Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D010618:Phenanthrolines; D011480:Protease Inhibitors; D015842:Serine Proteinase Inhibitors; D020778:Matrix Metalloproteinase 2; D015032:Zinc; D002118:Calcium; C025205:1,10-phenanthroline