title: A T7 Promoter-based Versatile Expression Vector System.
abstract: A family of T7 promoter-based versatile expression plamides for E. coli Were constructed. These vectors were featured strong T7 promoter, translational start, stop elements, a multiple cloning site with eight unique restriction sites in all three reading frames and f1 phage origin which allows packaging of single stranded plasmid, mutagensis and gene sequencing without subcloning steps. With these vectors recombinant proteins can be expressed in mature or short fusion forms. Many heterolegous genes have been highly expressed using these vectors, most of them were expressed in soluble and active forms.
authors.affiliation: Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
authors.forename: Chang-Zheng
authors.initials: CZ
authors.lastname: Chen
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Duan
authors.forename: Xin-Ying
authors.initials: XY
authors.lastname: Yang
authors.forename: Qi-Chang
authors.initials: QC
authors.lastname: Xia
authors.forename: Bo-Liang
authors.initials: BL
authors.lastname: Li
authors.forename: Ying-Lai
authors.initials: YL
authors.lastname: Wang