Enhancement of NK cytotoxicity, antimetastasis and elongation effect of survival time in B16-F10 melanoma cells by oregonin.
We investigated the antitumor activity of oregonin, a diarylheptanoid derivative purified from Alnus hirsuta Turcz, Betulaceae. Oregonin is a potential novel immunomodulator, which augments the activation of natural killer (NK) cells, and thereby leads to a powerful antitumor activity. To evaluate the cytotoxicity of oregonin against tumor cells, we examined the effectiveness of NK cells and determined that oregonin could increase NK cell cytotoxicity. This was confirmed by MTT assay. In addition, the survival time of C57BL/6 mice were measured by inoculating B16-F10 melanoma cells to mice via intra muscular (i.m.) injection. Oregonin treatment after 10 hours of inoculation at 10 mg/kg dosage showed a significant extension of survival time by up to 51.32%, when compared to the control group. Moreover, oregonin significantly reduced the incidence of pulmonary metastasis, which may be developed from B16-F10 melanoma cells. These findings suggest that oregonin may be classified as a new and novel immunomodulator due to its potential antitumor activity.
Joo
Seong-Soo
SS
Division of Immunology College of Pharmacy, Chungang University, Seoul, Korea.
Kim
Min-Soo
MS
Oh
Won-Sik
WS
Lee
Do-Ik
DI
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic; D036381:Diarylheptanoids / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects*; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D009362:Neoplasm Metastasis / Q000473:pathology / Q000517:prevention & control*; D013268:Stimulation, Chemical; D013534:Survival; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D036381:Diarylheptanoids; D013778:Tetrazolium Salts; D013844:Thiazoles; C409456:oregonin; C022616:thiazolyl blue