YIL113w encodes a functional dual-specificity protein phosphatase which specifically interacts with and inactivates the Slt2/Mpk1p MAP kinase in S. cerevisiae.
We show here that the YIL113w gene of Saccharomyces cerevisiae encodes a functional protein phosphatase. Yil113p shows no activity in vitro towards either phosphorylated casein or myelin basic protein. However, Yil113p dephosphorylates activated extracellular signal-regulated kinase 2 MAP kinase indicating that it is a dual-specificity MAP kinase phosphatase. In support of this we find that Yil113p specifically interacts with the stress-activated Slt2/Mpk1p MAP kinase of S. cerevisiae. Furthermore, expression of Yil113p causes the dephosphorylation of Slt2/Mpk1p in vivo, while expression of an inactive mutant of Yil113p causes the accumulation of phosphorylated Slt2/Mpk1p. We conclude that the physiological target of YIL113p is Slt2/Mpk1p.
Collister
Michelle
M
Cancer Research UK, Molecular Pharmacology Unit, Biomedical Research Centre, Ninewells Hospital, DD1 9SY, Dundee, UK
Didmon
Mark P
MP
MacIsaac
Fiona
F
Stark
Michael J
MJ
MacDonald
Neil Q
NQ
Keyse
Stephen M
SM
D054637:Dual-Specificity Phosphatases; D004789:Enzyme Activation; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism*; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D020798:Two-Hybrid System Techniques
D029701:Saccharomyces cerevisiae Proteins; D020928:Mitogen-Activated Protein Kinases; C072724:SLT2 protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; D054637:Dual-Specificity Phosphatases; C467054:SDP1 protein, S cerevisiae