Effect of tea catechins on cellular lipid peroxidation and cytotoxicity in HepG2 cells.
Tea catechins inhibited TBARS accumulation in HepG2 cells, the order of effectiveness being (-)-epigallocatechin gallate (EGCG) > (-)-epigallocatechin (EGC) > or = (-)-epicatechin gallate (ECG) > (-)-epicatechin (EC). EGCG and EGC protected the depletion of alpha-tocopherol in the cells, and the glutathione content was enhanced by all four catechins. Moreover, all four catechins suppressed the formation of glutathione disulfide and the activation of glutathione peroxidase induced by tert-butylated hydroperoxide.
Murakami
Chikako
C
Faculty of Nutrition, Kobe Gakuin University, Japan.
Hirakawa
Yuki
Y
Inui
Hiroshi
H
Nakano
Yoshihisa
Y
Yoshida
Hiromi
H
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005978:Glutathione / Q000494:pharmacology; D019803:Glutathione Disulfide / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008113:Liver Neoplasms / Q000473:pathology; D013662:Tea / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000494:pharmacology; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D013662:Tea; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D002392:Catechin; C062669:epicatechin gallate; D020122:tert-Butylhydroperoxide; C045651:epigallocatechin gallate; D005979:Glutathione Peroxidase; D005978:Glutathione; C057580:gallocatechol; D019803:Glutathione Disulfide