title: Expression of the atpE Gene Coding for the epsilon Subunit of Maize Chloroplast Coupling Factor.
abstract: The entire atpE gene of the maize chloroplast coupling factor was inserted into the polylinker region of vectors pJLA505 and pWA to form recombinant plasmids pJLA505-atpE and pWA-atpE respectively. These expression plasmids were transformed into E. coli NM522 which induced at 42 degrees. By the analysis of SDS-PAGE, the expressed product of interest was observed to account fore more than 3o% of total E. coliproteins. The identification of the expressed product demonstrated that its immunological specificity was well retained. The antiserum cross-reacted with the expressed epsilon protein and CF(1)-epsilon protein of spinach and produced precipitin lines on Ouchterlony immunodiffusion test. The expressed product aggregated insolubly as the inclusion body and was purified to over 80% purity. The purified product had the same function as that of the native epsilon subunit.
authors.affiliation: Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai 200032, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Shi
authors.forename: Jian-Mian
authors.initials: JM
authors.lastname: Wei
authors.forename: Yun-Gang
authors.initials: YG
authors.lastname: Shen