title: Inactivation of the antibacterial activity of iodine potassium iodide and chlorhexidine digluconate against Enterococcus faecalis by dentin, dentin matrix, type-I collagen, and heat-killed microbial whole cells.
abstract: The antibacterial activity of chlorhexidine digluconate and iodine potassium iodide on Enterococcus faecalis A197A was tested in the presence of dentin, dentin matrix, dentin pretreated by EDTA and citric acid, collagen, and heat-killed cells of Enterococcus faecalis and Candida albicans. Medications were preincubated for 1 h with each of the potential inhibitors and tested for their antibacterial activity against E. faecalis, strain A197A. Surviving bacteria were sampled after 1 and 24 h of incubation. Dentin matrix and heat-killed microbial cells were the most effective inhibitors of chlorhexidine, whereas dentin pretreated by citric acid or EDTA showed only slight inhibition. Dentin and skin collagen showed some inhibition at 1 h but not after 24 h. Iodine potassium iodide was effectively inhibited by dentin, dentin matrix, and heat-killed microbial cells. Skin collagen and dentin pretreated by EDTA or by citric acid showed little or no inhibitory effect on iodine potassium iodide. Different components of dentin are responsible for the divergent patterns of inhibition of the antibacterial activity of chlorhexidine digluconate and iodine potassium iodide. Chemical treatment of dentin before applying the medication into the root canal may alter the antibacterial effect of the medication.
authors.affiliation: Department of Endodontics, Dental Faculty, University of Oslo, Norway.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Portenier
authors.forename: Hanna
authors.initials: H
authors.lastname: Haapasalo
authors.forename: Dag
authors.initials: D
authors.lastname: Orstavik
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Yamauchi
authors.forename: Markus
authors.initials: M
authors.lastname: Haapasalo
mesh_terms: D002176:Candida albicans / Q000502:physiology; D002614:Chelating Agents / Q000494:pharmacology; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors*; D019343:Citric Acid / Q000494:pharmacology; D024042:Collagen Type I / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003804:Dentin / Q000502:physiology*; D004202:Disinfectants / Q000037:antagonists & inhibitors*; D017886:Durapatite / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D017613:Iodine Compounds / Q000037:antagonists & inhibitors*; D011193:Potassium Iodide / Q000037:antagonists & inhibitors*; D012388:Root Canal Irrigants / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
chemical_list: D002614:Chelating Agents; D024042:Collagen Type I; D004202:Disinfectants; D017613:Iodine Compounds; D012388:Root Canal Irrigants; C439385:iodine potassium iodide; D011193:Potassium Iodide; D019343:Citric Acid; D017886:Durapatite; D004492:Edetic Acid; C010882:chlorhexidine gluconate; D002710:Chlorhexidine