title: Torovirus detection in faecal specimens of calves and pigs in Hungary: short communication.
abstract: Bovine torovirus is an established aetiological agent of disease in cattle, while porcine torovirus has only been isolated from healthy animals. Evidence for the presence of torovirus has been described in several European countries and also in the United States. A survey was performed to detect toroviruses in Hungary by means of sampling ten swine and nine bovine herds. Rectal swabs and faecal specimens were collected from diarrhoeic calves and from weaned piglets. The samples were tested by the reverse transcription-polymerase chain reaction (RT-PCR) using torovirus-specific primers and the positive samples were further examined by electron microscopy (EM). Torovirus was detected in 4 diarrhoeic calves (out of 111) and in 10 healthy weaned pigs (out of 200 tested), representing two of the 9 calf herds and two of the 10 pig herds tested. This is the first report of exact diagnosis of torovirus in Hungary.
authors.affiliation: Veterinary Institute of Debrecen, H-4002 Debrecen, P.O. Box 51, Hungary. matizk@oai.hu
authors.forename: Katalin
authors.initials: K
authors.lastname: Matiz
authors.forename: S
authors.initials: S
authors.lastname: Kecskeméti
authors.forename: I
authors.initials: I
authors.lastname: Kiss
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Adám
authors.forename: J
authors.initials: J
authors.lastname: Tanyi
authors.forename: B
authors.initials: B
authors.lastname: Nagy
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology; D017931:DNA Primers; D005243:Feces / Q000382:microbiology; D006814:Hungary / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology; D017003:Torovirus / Q000302:isolation & purification* / Q000648:ultrastructure; D018176:Torovirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology
chemical_list: D017931:DNA Primers