Costunolide induces differentiation of human leukemia HL-60 cells.
Costunolide has been reported to be a cytotoxic and chemopreventive agent. This work investigated the mechanism of the antiproliferative effect of costunolide and determined that it induced differentiation of the human leukemia cell line HL-60. Costunolide exhibited a potent antiproliferative activity against HL-60 cells. It was also found to be a potent inducer of differentiation in human leukemia derived HL-60 cells through the examination of differentiation markers, as assessed by the reduction of nitroblue-tetrazolium, the increase in esterase activities and phagocytic activity, morphology change and the expression of CD14 and CD66b surface antigens. These results, accompanied by a decline in the expression of c-myc protein, suggest that costunolide induces differentiation of human leukemia cells to granulocytes and monocytes/macrophages lineage.
Choi
Jung-Hye
JH
College of Pharmacy, Kyung Hee University, Seoul, Korea.
Seo
Bo-Rim
BR
Seo
Seong-Hoon
SH
Lee
Kyung-Tae
KT
Park
Jae-Hoon
JH
Park
Hee-Juhn
HJ
Choi
Jong-Won
JW
Itoh
Yoshie
Y
Miyamoto
Ken-ichi
K
D015703:Antigens, CD; D000951:Antigens, Neoplasm / Q000096:biosynthesis; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects; D005434:Flow Cytometry; D058851:GPI-Linked Proteins; D018922:HL-60 Cells; D006801:Humans; D007202:Indicators and Reagents; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D009580:Nitroblue Tetrazolium; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D012717:Sesquiterpenes / Q000494:pharmacology*; D007783:Lactones
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000972:Antineoplastic Agents, Phytogenic; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D007202:Indicators and Reagents; D018950:Lipopolysaccharide Receptors; D009580:Nitroblue Tetrazolium; D016271:Proto-Oncogene Proteins c-myc; D012717:Sesquiterpenes; C504120:CEACAM8 protein, human; C002602:costunolide; D007783:Lactones