Enantiomer separation of flavour and fragrance compounds by liquid chromatography using novel urea-covalent bonded methylated beta-cyclodextrins on silica.
A novel methylated beta-cyclodextrin chiral stationary phase (CSP-ME), which was chemically immobilised onto porous silica via multiple urea-linkages was synthesised. The CSP-ME chiral stationary phase depicted good enantiomer separation abilities for some well-known flavour as well as fragrance compounds using high-performance liquid chromatography under reverse phase conditions. The optimum resolution for alpha-ionone, 3-methyl-alpha-ionone, flavanone, 5-methoxyflavanone, 6-methoxyflavanone, 7-methoxyflavanone, hesperetin, naringenin and taxifolin was achieved using a mobile phase composition consisting of 1 wt.% triethylammonium acetate buffer (pH 4.68)-methanol. The effects of pH of triethylammonium acetate buffer and the methanol-acetonitrile content of the mobile phase composition on their retention time and resolution were examined to optimise the separation conditions.
Ng
Siu-Choon
SC
Department of Chemistry, National University of Singapore, Singapore. chmngsc@leonis.nus.edu.sg
Ong
Teng-Teng
TT
Fu
Ping
P
Ching
Chi-Bun
CB
D002853:Chromatography, Liquid / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry*; D005421:Flavoring Agents / Q000302:isolation & purification*; D008745:Methylation; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D005421:Flavoring Agents; D047392:beta-Cyclodextrins; D012822:Silicon Dioxide; C031215:betadex