title: Protease-catalyzed peptide synthesis on solid support.
abstract: The direct enzymatic synthesis of peptides from amino acids is widely used as a useful alternative to chemical synthesis. However, good yields of such enzyme-catalyzed reactions require altered reaction conditions to overcome the bias for hydrolysis in aqueous medium. We argue that the synthesis/hydrolysis equilibrium can be shifted toward synthesis in aqueous medium by immobilizing the amine on solid support. In this report, we show the first examples of solid-phase peptide synthesis catalyzed by a protease in bulk aqueous buffer.
authors.affiliation: Department of Chemistry, The University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3JJ, Scotland.
authors.forename: Rein V
authors.initials: RV
authors.lastname: Ulijn
authors.forename: Beatriz
authors.initials: B
authors.lastname: Baragaña
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Halling
authors.forename: Sabine L
authors.initials: SL
authors.lastname: Flitsch
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D002384:Catalysis; D002625:Chemistry, Organic / Q000379:methods; D010450:Endopeptidases / Q000737:chemistry* / Q000378:metabolism; D005449:Fluorenes / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis*; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D000178:Acrylamides; D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010455:Peptides; D011092:Polyethylene Glycols; D010450:Endopeptidases