Enhancing and Inhibiting Effect of Vitamin C on Lipid Peroxidation in the Linoleic Acid Micelle System.
Vitamin C (Vc), a reductant in the body can function as an antioxidant by scavenging free radicals. However, with the coexistence of the transition metal iron (Fe), Vc can induce lipid peroxidation reaction resulting in the production of conjugated diene which has a peek absorbance at UV 234 nm. In order to clarify the proportion of Vc to Fe on the two different effects mentioned above, the authors detected conjugated diene production by means of spectrophotometer in linoleic acid micelles induced by Fe with different amount of Vc. The results showed that in the presence of Te or Vc alone there was no induction of lipid peroxidation. In the presence of some Fe (20 &mgr;M), Vc induced lipid peroxidation at a concentration of 80 &mgr;M or lower, but inhibited lipid peroxidation at concentration of 100 &mgr;M or higher. The pH of the experimental system clearly affected the lipid peroxidation induced by Fe-Vc, with the production of conjugated diene at acidic pH, but none at physiological pH.
Yu
Ying-Yan
YY
Department of Pathology, the Second Affiliated Hospital of Hebei Medical College, Shijiazhuang 050000, China.
Okada
Shigeru
S