title: Genetic interactions link ARF1, YPT31/32 and TRS130.
abstract: A genetic screen for synthetic lethal interactions with arf1(-) identified a recessive mutation in TRS130, one of 10 components in the trafficking protein particle (TRAPP) complex (Sacher et al., 2000). As TRS130 is an essential gene, the synthetic lethal allele (trs130-101) is a novel one that requires ARF1 for viability. This allele was found to exhibit no defects in secretory function, i.e. processing of carboxypeptidase Y or invertase. YPT31 and YPT32 were identified in a subsequent screen as high-copy suppressors of arf1(-)trs130-101. Increasing the gene dosage of YPT31/32 also suppressed lethality resulting from deletion of TRS130 or TRS120 but not three other essential TRAPP subunit-encoding genes. Although unable to suppress defects in several alleles of ARF1, increasing the gene dosage of YPT31/32 suppressed the cold sensitivity of gcs1(-), an Arf GTPase-activating protein (GAP). Thus, these genetic interactions provide initial evidence for linkage of Arf and TRAPP signalling and for Ypt31/32 proteins functioning downstream of both components in the TRAPP complex and of Arf signalling via the Gcs1 Arf GAP.
authors.affiliation: Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322-3050, USA.
authors.forename: Chun-jiang
authors.initials: CJ
authors.lastname: Zhang
authors.forename: J Bradford
authors.initials: JB
authors.lastname: Bowzard
authors.forename: Michelle
authors.initials: M
authors.lastname: Greene
authors.forename: Aimee
authors.initials: A
authors.lastname: Anido
authors.forename: Katherine
authors.initials: K
authors.lastname: Stearns
authors.forename: Richard A
authors.initials: RA
authors.lastname: Kahn
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000235:genetics* / Q000378:metabolism; D000483:Alleles; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D020690:GTPase-Activating Proteins; D018628:Gene Dosage; D015966:Gene Expression Regulation, Fungal; D016153:Genes, Suppressor; D006026:Glycoside Hydrolases / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D033921:Vesicular Transport Proteins; D015003:Yeasts / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D043324:beta-Fructofuranosidase; D020691:rab GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C089147:GCS1 protein, S cerevisiae; D020690:GTPase-Activating Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; C406327:transport protein particle, TRAPP; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; C087905:YPT31 protein, S cerevisiae; C121633:YPT32 protein, S cerevisiae; D020823:ADP-Ribosylation Factor 1; D020691:rab GTP-Binding Proteins