Cloning and characterization of SLP3: a novel member of the stomatin family expressed by olfactory receptor neurons.
The detection of odorants with high sensitivity and specificity utilizes specialized transduction proteins that may be assembled into complexes to afford enhanced speed and efficiency in olfactory neurons. We have used a differential cDNA screening technique to identify novel gene products that display restricted expression within the olfactory epithelium. Here we report the characterization of an olfactory neuronal protein, SLP3, which shares extensive homology with the stomatin family of membrane proteins. Other stomatin family members have been implicated in specific interactions with ion channels and G protein-coupled receptors. The pattern of SLP3 mRNA expression during embryonic development and the subcellular localization of the SLP3 protein in mature olfactory neurons observed here is consistent with a specific role for this protein in the assembly, translocation, or function of the odorant transduction complex in olfactory neurons.
Goldstein
Bradley J
BJ
Department of Otolaryngology and Head and Neck Surgery, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Kulaga
Heather M
HM
Reed
Randall R
RR
D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics*; D009833:Olfactory Pathways / Q000502:physiology; D018034:Olfactory Receptor Neurons / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D001798:Blood Proteins; D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; C488964:Stom protein, mouse; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C486764:STOML3 protein, human; C461699:Stoml3 protein, mouse