title: Coordinate binding studies of the substrate (factor X) with the cofactor (factor VIII) in the assembly of the factor X activating complex on the activated platelet surface.
abstract: The assembly of the factor X activating complex on the platelet surface requires the occupancy of three receptors: (1) enzyme factor IXa, (2) cofactor factor VIII(a), and (3) substrate factor X. To further evaluate this three-receptor model, simultaneous binding isotherms of (125)I-factor X and (131)I-factor VIII(a) to activated platelets were determined as a function of time and also as a function of the concentrations of both ligands in the presence of active site-inhibited factor IXa (45 nM) and 5 mM CaCl(2). In the presence of active site-inhibited factor IXa and factor VIIIa there are two independent factor X binding sites: (1) low affinity, high capacity (approximately 9000 sites/platelet; K(d) approximately 380 nM) and (2) low capacity, high affinity (1700 sites/platelet; K(d) approximately 30 nM). A single specific and selective factor X binding site was expressed (1200 sites/platelet; K(d) approximately 9 nM) when the shared factor X/factor II site was blocked by excess factor II (4 microM). In the presence of active site-inhibited factor IXa (4 nM) and factor II (4 microM), factor X binds to 3-fold more platelet sites than procofactor VIII with relatively low affinity (K(d) approximately 250 nM). The activation of procofactor VIII to factor VIIIa increases the affinity of binding to platelets of both factor VIIIa ( approximately 4-fold to K(d) approximately 0.8-1.5 nM) and factor X ( approximately 25-50-fold to K(d) approximately 5-9 nM). In the presence of excess zymogen factor IX, which blocks the shared factor IX/factor IXa binding site, the substrate, factor X, and the active cofactor, factor VIIIa, form a 1:1 stoichiometric complex. These coordinate binding studies support the conclusion that factor X initially binds to a high-capacity, low-affinity platelet binding site shared with prothrombin, which then presents factor X to a specific high-affinity site consisting of factor VIIIa bound to a high-affinity, low-capacity receptor on activated platelets.
authors.affiliation: The Sol Sherry Thrombosis Research Center and Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
authors.forename: Syed S
authors.initials: SS
authors.lastname: Ahmad
authors.forename: Peter N
authors.initials: PN
authors.lastname: Walsh
mesh_terms: D001665:Binding Sites; D001780:Blood Coagulation Tests; D001792:Blood Platelets / Q000201:enzymology / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004792:Enzyme Precursors / Q000378:metabolism; D005164:Factor IX / Q000378:metabolism; D015949:Factor IXa / Q000378:metabolism; D005169:Factor VIII / Q000378:metabolism*; D015944:Factor VIIIa / Q000378:metabolism; D005170:Factor X / Q000378:metabolism*; D015951:Factor Xa / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes / Q000378:metabolism; D046911:Macromolecular Substances; D015539:Platelet Activation; D011485:Protein Binding; D011516:Prothrombin / Q000378:metabolism; D011869:Radioligand Assay; D013379:Substrate Specificity
chemical_list: C408471:5-dimethylaminonaphthalene-1-sulfonyl glutamyl-glycyl-arginyl chloromethyl ketone inactivated factor IXa; D004792:Enzyme Precursors; D007457:Iodine Radioisotopes; D046911:Macromolecular Substances; D015944:Factor VIIIa; D011516:Prothrombin; D005169:Factor VIII; D005164:Factor IX; D005170:Factor X; D015949:Factor IXa; D015951:Factor Xa