title: The FLEXGene repository: exploiting the fruits of the genome projects by creating a needed resource to face the challenges of the post-genomic era.
abstract: Thanks to the results of the multiple completed and ongoing genome sequencing projects and to the newly available recombination-based cloning techniques, it is now possible to build gene repositories with no precedent in their composition, formatting, and potential. This new type of gene repository is necessary to address the challenges imposed by the post-genomic era, i.e., experimentation on a genome-wide scale. We are building the FLEXGene (Full Length EXpression-ready) repository. This unique resource will contain clones representing the complete ORFeome of different organisms, including Homo sapiens as well as several pathogens and model organisms. It will consist of a comprehensive, characterized (sequence-verified), and arrayed gene repository. This resource will allow full exploitation of the genomic information by enabling genome-wide scale experimentation at the level of functional/phenotypic assays as well as at the level of protein expression, purification, and analysis. Here we describe the rationale and construction of this resource and focus on the data obtained from the Saccharomyces cerevisiae project.
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Institute of Proteomics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Brizuela
authors.forename: Aaron
authors.initials: A
authors.lastname: Richardson
authors.forename: Gerald
authors.initials: G
authors.lastname: Marsischky
authors.forename: Joshua
authors.initials: J
authors.lastname: Labaer
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D019295:Computational Biology; D030541:Databases, Genetic; D016678:Genome; D015894:Genome, Human; D023281:Genomics; D006801:Humans; D016366:Open Reading Frames; D040901:Proteomics