title: Cultivation-dependent characterization of bacterial diversity from British Columbia forest soils subjected to disturbance.
abstract: Bacteria from forest surface organic matter and mineral soil horizons were cultivated using four methods and characterized by fatty acid methyl ester (FAME) analysis. Soil samples from a British Columbia Ministry of Forests Long-Term Soil Productivity (LTSP) installation were collected during winter and summer from two disturbance treatments (whole-tree harvesting with no soil compaction (plot N) and whole-tree harvesting plus complete surface organic matter removal with heavy soil compaction (plot S)) and from an unlogged reference plot (REF). Seventy-five percent of 1795 bacterial isolates were affiliated with 42 genera representing beta- and gamma-Proteobacteria, Actinobacteria, the Bacillus/Clostridium group, and the Cytophaga-Flexibacter-Bacteroides group. Approximately half of the culture collection represented genetic diversity confined to four bacterial genera: Pseudomonas, Bacillus, Paenibacillus, and Arthrobacter. A significantly higher proportion of bacterial isolates belonging to Actinobacteria, and the member genus Arthrobacter, were isolated from plot S soil samples compared with soil samples from plots N and REF. Twenty-five percent of bacterial isolates were not conclusively identified to genus with FAME analysis. Sherlock Tracker cluster analysis and partial 16S rRNA gene sequence analysis enabled classification of a subset of these isolates.
authors.affiliation: BC Research Inc, Vancouver, Canada. paxelrood@bcresearch.com
authors.forename: Paige E
authors.initials: PE
authors.lastname: Axelrood
authors.forename: Monica L
authors.initials: ML
authors.lastname: Chow
authors.forename: Clarke S
authors.initials: CS
authors.lastname: Arnold
authors.forename: Karen
authors.initials: K
authors.lastname: Lu
authors.forename: Joseph M
authors.initials: JM
authors.lastname: McDermott
authors.forename: Julian
authors.initials: J
authors.lastname: Davies
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001955:British Columbia; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D017753:Ecosystem; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D014644:Genetic Variation; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil