title: Lipoatrophic diabetes-associated utero-ovarian dysfunction: influence of cellular lipid deposition on norepinephrine indices.
abstract: OBJECTIVE
  Elucidation of the intracellular lipoatrophic diabetic state and the concomitant alterations in norepinephrine (NE) parameters characterizing female reproductive failure.
  
  
  METHODS
  Quantitation of intrinsic NE levels in utero-ovarian and pancreatic tissue samples of C57BL/KsJ (+/?) control and (db/db) diabetic littermate mice was by high performance liquid chromatography (HPLC) and compared with the microspectrofluorometric histofluorescent (HF) localization of cellular and parenchymal NE.
  
  
  RESULTS
  Diabetes-associated elevations in HPLC-detectable tissue NE concentrations occurred in all pancreatic and reproductive tract tissue samples as compared to control-matched samples, whereas concurrent HF analysis revealed suppressed perivascular and parenchymal NE depositions in diabetic mice.
  
  
  CONCLUSIONS
  These data suggest that progressive hypertriglyceridemia/lipidemia may suppress the effectiveness of intrinsic elevations in tissue NE concentrations from effectively counterregulating the deleterious effects of the hyperglycemic, type-2 diabetic condition.
authors.affiliation: Division of Cell Biology and Biophysics, School of Biological Sciences and School of Medicine, University of Missouri-Kansas City, Mo. 64110-2499, USA. garrisd@umkc.edu
authors.forename: David R
authors.initials: DR
authors.lastname: Garris
authors.forename: Bryan L
authors.initials: BL
authors.lastname: Garris
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003593:Cytoplasm / Q000648:ultrastructure; D003923:Diabetes Mellitus, Lipoatrophic / Q000473:pathology / Q000503:physiopathology*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000473:pathology; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005260:Female; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D009638:Norepinephrine / Q000032:analysis*; D015388:Organelles / Q000648:ultrastructure; D010053:Ovary / Q000737:chemistry / Q000503:physiopathology* / Q000648:ultrastructure; D010179:Pancreas / Q000737:chemistry; D014280:Triglycerides / Q000097:blood; D014599:Uterus / Q000737:chemistry / Q000503:physiopathology* / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure; D015430:Weight Gain
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D008055:Lipids; D014280:Triglycerides; D009638:Norepinephrine