title: Appearance and phenotype of murine follicular dendritic cells expressing VCAM-1.
abstract: The architecture of lymphoid follicles is determined by a series of interactions between lymphoid and follicular stromal cells. A cardinal population in the non-lymphoid compartment is the follicular dendritic cell (FDC), whose communication with resting and activated B cells involves various adhesive interactions. The FDC phenotype variably includes the display of vascular cell adhesion molecule (VCAM-1). In this report we investigated the appearance and follicular tissue distribution of VCAM-1 in murine peripheral lymphoid tissues, and compared VCAM-1 with other FDC markers using immunohistochemistry. Correlating the appearance of VCAM-1 with other murine FDC-associated markers (CR1.2 [complement receptor 1.2 or CD35/21] and FDC-M1) revealed that the display of VCAM-1 is restricted to a subset of CR1.2-positive FDCs. We found that the expression of VCAM-1 antigen in the spleen or peripheral lymph nodes on FDCs requires antigenic stimulus, and that it coincides with germinal center formation. The VCAM-1 expression is associated with the appearance of mucosal addressin cell adhesion molecule (MAdCAM-1), with some slight differences in occurrence. The appearance of VCAM-1 and MAdCAM-1 antigens on FDCs may serve as indicators of FDC activation.
authors.affiliation: Immunology Group, Department of Anatomy and Neurobiology, Medical College of Virginia/VCU, Richmond, VA 23298, USA.
authors.forename: Péter
authors.initials: P
authors.lastname: Balogh
authors.forename: Yüksel
authors.initials: Y
authors.lastname: Aydar
authors.forename: John G
authors.initials: JG
authors.lastname: Tew
authors.forename: Andras K
authors.initials: AK
authors.lastname: Szakal
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D015815:Cell Adhesion Molecules; D003713:Dendritic Cells / Q000737:chemistry* / Q000378:metabolism; D000939:Epitopes; D005434:Flow Cytometry; D007136:Immunoglobulins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009088:Mucoproteins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D010581:Peyer's Patches / Q000737:chemistry; D010641:Phenotype; D013154:Spleen / Q000737:chemistry; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D015815:Cell Adhesion Molecules; D000939:Epitopes; D007136:Immunoglobulins; C489082:Madcam1 protein, mouse; D009088:Mucoproteins; D019010:Vascular Cell Adhesion Molecule-1