title: The effect of Chinese medicine on bone cell activities.
abstract: In this experiment, we investigate the biochemical effects of traditional Chinese medicines via an in vitro bone cell culture. Ten different Chinese medicines were used in this study. The rat osteoblast-osteoclast co-culture system was used as the experimental model. After the cells grew to 80% confluence, various tested materials were added. The mitochondria activity of the bone cells after exposure to various preparations of Chinese medicines was determined by colorimetric assay. Biochemical markers such as protein content, lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and acid phosphatase (ACP) titer were analyzed to evaluate the bone cell activity. When cultured with various Chinese medicines for 24 hours, only four of these ten Chinese medicines had potential beneficial effects on the bone cell culture; and only Drynaria fortunei (Kunze) J. Sm. had a universal beneficial effect on bone cell metabolism. The major beneficial effect of Drynaria fortunei (Kunze) J. Sm. on bone cells is probably mediated by the induction of apoptosis of the osteoclast cell population. Continued study of alterations in gene expression of bone cells caused by Chinese medicines will improve our understanding of bone cell responses to various pharmacological interventions.
authors.affiliation: School of Pharmacy, Taipei Medical University, Taiwan, ROC.
authors.forename: Chuu-Yu
authors.initials: CY
authors.lastname: Lin
authors.forename: Jui-Sheng
authors.initials: JS
authors.lastname: Sun
authors.forename: Shiow-Yunn
authors.initials: SY
authors.lastname: Sheu
authors.forename: Feng-Huei
authors.initials: FH
authors.lastname: Lin
authors.forename: Yng-Jiin
authors.initials: YJ
authors.lastname: Wang
authors.forename: Li-Ting
authors.initials: LT
authors.lastname: Chen
mesh_terms: D000135:Acid Phosphatase / Q000187:drug effects; D000469:Alkaline Phosphatase / Q000187:drug effects; D000818:Animals; D002460:Cell Line / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007150:Immunohistochemistry; D007770:L-Lactate Dehydrogenase / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D008517:Phytotherapy; D029621:Polypodiaceae; D051381:Rats
chemical_list: D004365:Drugs, Chinese Herbal; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase