Macro-alkaline phosphatase due to IgG kappa complex: demonstration with polyethylene glycol precipitation and immunofixation.
An otherwise unexplained, persistently elevated plasma alkaline phosphatase concentration in a 71-year-old woman was found to be attributable to the presence of macro-alkaline phosphatase using polyethylene glycol precipitation. Gel filtration showed two high MW peaks with masses of about 330 kDa and 560 kDa. The alkaline phosphatase (ALP) complex was characterized by immunoelectrophoresis as a complex with IgG with kappa light chains.
Owen
M C
MC
Clinical Biochemistry Unit, Canterbury Health Laboratories, Christchurch, New Zealand. Maurice.Owen@chmeds.ac.nz
Pike
L S
LS
George
P M
PM
Barclay
M L
ML
Florkowski
C M
CM
D000368:Aged; D000469:Alkaline Phosphatase / Q000097:blood* / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000276:immunology*; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000097:blood* / Q000276:immunology; D007527:Isoenzymes / Q000097:blood / Q000276:immunology; D011092:Polyethylene Glycols
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007527:Isoenzymes; D011092:Polyethylene Glycols; D000469:Alkaline Phosphatase