Portal absorption of 14C after ingestion of spiked milk with 14C-phenanthrene, 14C-benzo[a]pyrene or 14C-TCDD in growing pigs.
Polycyclic aromatic hydrocarbons (PAHs) and dioxins are lipophilic organic pollutants occurring widely in the terrestrial environment. In order to study the PAHs and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) transfer in the food chain, pigs have been fed with milk mixed either with 14C-phenanthrene, with 14C-benzo[a]pyrene or with 14C-TCDD. The analysis of portal and arterial blood radioactivity showed that both PAHs and TCDD were absorbed with a maximum concentration at 4-6 h after milk ingestion. Then, the blood radioactivity decreased to reach background levels 24 h after milk ingestion. Furthermore, the portal and arterial blood radioactivities were higher for phenanthrene (even if the injected load was the lowest) than these of benzo[a]pyrene or these of TCDD, in agreement with their lipophilicity and water solubility difference. Main 14C absorption occurred during the 1-3 h time period after ingestion for 14C-phenanthrene and during the 3-6 h time period for 14C-benzo[a]pyrene and for 14C-TCDD. 14C portal absorption rate was high for 14C-phenanthrene (95%), it was close to 33% for 14C-benzo[a]pyrene and very low for 14C-TCDD (9%). These results indicate that the three studied molecules have a quite different behaviour during digestion and absorption. Phenanthrene is greatly absorbed and its absorption occurs via the blood system, whereas benzo[a]pyrene and TCDD are partly and weakly absorbed respectively. However these two molecules are mainly absorbed via the portal vein.
Laurent
Claire
C
Laboratoire Sciences Animales, INPL-UHP-INRA, Ecole Nationale Supérieure d'Agronomie et des Industries Alimentaires, University of Nancy I, Vandoeuvre les Nancy, France.
Feidt
Cyril
C
Grova
Nathalie
N
Mpassi
Didier
D
Lichtfouse
Eric
E
Laurent
François
F
Rychen
Guido
G
D000042:Absorption; D000818:Animals; D001564:Benzo(a)pyrene / Q000493:pharmacokinetics*; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D004064:Digestive System; D008892:Milk / Q000737:chemistry; D000072317:Polychlorinated Dibenzodioxins / Q000493:pharmacokinetics*; D013552:Swine; D013723:Teratogens / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D002250:Carbon Radioisotopes; D000072317:Polychlorinated Dibenzodioxins; D013723:Teratogens; D001564:Benzo(a)pyrene