Correlation between cellular localization of TEL/AML1 fusion protein and repression of AML1-mediated transactivation of CR1 gene.
Human chromosome translocation t(12;21)(p12;q22) is the most frequent chromosome rearrangement in childhood B-lineage acute lymphoblastic leukemia (ALL), and produces the TEL/AML1 fusion protein. The chimeric protein, TEL/AML1 contains the first 336 amino acids of TEL that is linked to residues 21-480 of AML1 and the fusion protein is generally known as a transcription repressor to the various target genes. Furthermore, TEL/AML1 has been shown to interfere with AML1-mediated transactivation on the CR1 gene. To understand the mechanism of the TEL/AML1-mediated repression, we used transient-transfection assay and immunofluorescence to monitor subcellular localization of TEL/AML1. Here, we show that TEL/AML1 is localized in the cytoplasm and the transcriptional activities of CR1 promoter are affected by the subcellular localization of TEL/AML1 fusion protein.
Rho
Jae Kyun
JK
Department of Biology, School of Life Sciences, Research Center for Bioresource and Health, Chungbuk National University Cheongju, 361-764, Chungbuk, Republic of Korea.
Kim
Jae Hyun
JH
Yu
Jiyeon
J
Choe
Soo Young
SY
D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D017434:Protein Structure, Tertiary; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D017463:Receptors, Complement 3b / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D013223:Statistics as Topic; D013347:Subcellular Fractions; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D015533:Transcriptional Activation / Q000187:drug effects*; D014407:Tumor Cells, Cultured; D000097764:ETS Translocation Variant 6 Protein
D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D017463:Receptors, Complement 3b; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D000097764:ETS Translocation Variant 6 Protein; C493728:RUNX1 protein, human