Interaction of plexin-B1 with PDZ domain-containing Rho guanine nucleotide exchange factors.
The Rho family GTPase has been implicated in plexin-B1, a receptor for Semaphorin 4D (Sema4D), mediating signal transduction. Rho may also play a function in this signaling pathway as well as Rac, but the mechanisms for Rho regulation are poorly understood. In this study, we have identified two kinds of PDZ domain-containing Rho-specific guanine nucleotide exchange factors (RhoGEFs) as proteins interacting with plexin-B1 cytoplasmic domain. These PDZ domain-containing RhoGEFs showed significant homology to human KIAA0380 (PDZ-RhoGEF) and LARG (KIAA0382), respectively. Both KIAA0380 and LARG could bind plexin-B1 and a deletion mutant analysis of plexin-B1, KIAA0380 and LARG revealed that KIAA0380 and LARG bound plexin-B1 cytoplasmic tail through their PDZ domains. The tissue distribution analysis indicated that plexin-B1 was co-localized with KIAA0380 and LARG in various tissues. Immunocytochemical analysis showed that LARG was recruited to plasma membrane by plexin-B1. These results suggest that PDZ domain-containing RhoGEFs play a role in Sema4D-plexin-B1 mediating signal transduction.
Hirotani
Mutsumi
M
Group of Neurobiology, School of Allied Health Sciences, Osaka University Faculty of Medicine, Yamadaoka 1-7, Suita-shi, Osaka 565-0871, Japan.
Ohoka
Yoshiharu
Y
Yamamoto
Takahiro
T
Nirasawa
Hiromi
H
Furuyama
Tatsuo
T
Kogo
Mikihiko
M
Matsuya
Tokuzo
T
Inagaki
Shinobu
S
D000818:Animals; D001665:Binding Sites; D002478:Cells, Cultured; D015870:Gene Expression; D020662:Guanine Nucleotide Exchange Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000378:metabolism*; D064067:Rho Guanine Nucleotide Exchange Factors; D014162:Transfection
C577234:ARHGEF11 protein, human; C577222:ARHGEF12 protein, human; C577356:ARHGEF2 protein, human; D020662:Guanine Nucleotide Exchange Factors; D009419:Nerve Tissue Proteins; C492119:PLXNB1 protein, human; D011956:Receptors, Cell Surface; D064067:Rho Guanine Nucleotide Exchange Factors