Effect of nitrite on interaction between the giant freshwater prawn Macrobrachium rosenbergii and its pathogen Lactococcus garvieae.
Addition of nitrite-N at 1.5 mg l(-1) in tryptic soy broth (TSB) significantly (p < 0.05) decreased the growth rate of the bacterial pathogen Lactococcus garvieae and significantly (p < 0.05) reduced mortality compared to zero nitrite controls when injected into giant freshwater prawns Macrobrachium rosenbergii at 5 x 10(5) colony-forming units (CFU) per prawn. In other experiments, whereby prawns were injected with TSB-grown L. garvieae (5 x 10(5) CFU prawn(-1)) and then held in water containing nitrite-N, mortality at 72 h post-injection was significantly (p < 0.05) higher for prawns held in water containing 1.68 mg l(-1) nitrite than at lower concentrations. Prawns exposed to different concentrations of nitrite-N were examined for THC (total hemocyte count), phenoloxidase activity, respiratory burst, phagocytic activity and bacterial clearance efficiency. No significant differences in THC and phenoloxidase activity were observed among treatments. With prawns exposed to nitrite-N for 168 h (7 d) at 1.59 mg l(-1), phagocytic activity and clearance efficiency decreased, while at 1.15 mg l(-1) or more, respiratory burst increased, generating the superoxide anion at levels considered cytoxic to the host. We conclude that nitrite-N at 1.68 mg l(-1) causes depression in the immune response and increased mortality in M. rosenbergii infected with L. garvieae.
Cheng
Winton
W
Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, Taiwan, ROC.
Liu
Chun-Hung
CH
Chen
Jiann-Chu
JC
D000818:Animals; D015169:Colony Count, Microbial; D004198:Disease Susceptibility / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D006434:Hemocytes / Q000187:drug effects; D017045:Lactococcus / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009573:Nitrites / Q000494:pharmacology*; D033541:Palaemonidae / Q000187:drug effects / Q000276:immunology / Q000382:microbiology*; D010587:Phagocytosis / Q000187:drug effects; D016897:Respiratory Burst / Q000187:drug effects; D014774:Virulence
D009573:Nitrites; D014442:Monophenol Monooxygenase