title: Cell migration and proliferation are not discriminatory factors in the in vitro sociologic behavior of bronchial epithelial cell lines.
abstract: A model of cellular cohesion has been developed, which permits the in vitro study of the spatial and temporal distribution of two human bronchial cell lines. The spatial distribution of cells in culture was characterized from videomicroscopic recordings and analyzed using an algorithmic program of cellular sociology based on the use of three geometrical models: Voronoï's partition, Delaunay's graph, the and minimum spanning tree (MST). The results obtained suggested that the manner of cellular cohesiveness could be used to differentiate between the organizational behaviors of the cell lines: non-invasive 16HBE14o- cells rapidly formed clusters with a cohesive organization, whereas invasive BZR cells remained isolated and were characterized by a non-cohesive organization. Videomicroscopic and image analysis techniques also demonstrated that cell migration and proliferation are not discriminatory factors for explaining differences in the spatial organizations of the two cell lines. We concluded that the random nature of cell movement combined with the cell adhesion capacity are determinant factors in cell cluster formation.
authors.affiliation: INSERM U514, IFR53, Université de Reims, Reims, France.
authors.forename: Manuela
authors.initials: M
authors.lastname: Matos
authors.forename: Béatrice Nawrocki
authors.initials: BN
authors.lastname: Raby
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Zahm
authors.forename: Myriam
authors.initials: M
authors.lastname: Polette
authors.forename: Philippe
authors.initials: P
authors.lastname: Birembaut
authors.forename: Noël
authors.initials: N
authors.lastname: Bonnet
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D002449:Cell Aggregation / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology; D002455:Cell Division / Q000502:physiology*; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018715:Microscopy, Video; D008954:Models, Biological; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D020545:Respiratory Mucosa / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors