Selective separation of cis-trans geometrical isomers of beta-carotene via CO2 supercritical fluid extraction.
We investigated a novel method for the selective separation of beta-carotene isomers from a freeze-dried powder of the algae Dunaliella bardawil using supercritical fluid extraction. The separation method relies on the different dissolution rate of the 9Z and all-E isomers of beta-carotene in SC-CO(2). At first, the equilibrium solubility of the two isomers in SC-CO(2) was determined at the extraction conditions of 44.8 MPa and 40 degrees C. The solubility of the 9Z isomer was found to be nearly 4 times higher than that of the all-E isomer (1.92 x 10(-5) g all-E isomer/g CO(2) compared to 7.64 x 10(-5) g 9Z isomer/g CO(2)). When supercritical fluid extraction was applied to a carotenoid concentrate from the algae (29 wt% beta-carotene) or a freeze-dried powder of the algae (3.1% beta-carotene), a selective separation of the 9Z/all-E isomers of beta-carotene was obtained. Thirty-nine percent recovery of beta-carotene with 80% purity of 9Z isomer was achieved at the initial stages of extraction (40 mL CO(2)). The extraction rate of beta-carotene from the freeze-dried algae powder was slower than that from the carotenoid concentrate, resulting in a reduction in the recovery and purity of the 9Z isomer. This indicates that even at the initial stage of the extraction the internal mass resistance is significant. Isomer purity and recovery could be enhanced upon grinding of the algae powder.
Gamlieli-Bonshtein
Iris
I
Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
Korin
Eli
E
Cohen
Smadar
S
D002245:Carbon Dioxide; D000460:Chlorophyta / Q000378:metabolism*; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation / Q000379:methods*; D005240:Feasibility Studies; D005612:Freeze Drying / Q000379:methods; D011208:Powders; D012680:Sensitivity and Specificity; D012995:Solubility; D013237:Stereoisomerism; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000302:isolation & purification*
D011208:Powders; D019207:beta Carotene; D002245:Carbon Dioxide