title: Specific immunological detection of the (V+C)(-) fibronectin isoform.
abstract: The population of fibronectins in adult mammalian cartilage includes high levels of a cartilage-specific (V+C)(-) isoform which lacks the V, III-15, and I-10 segments and thus contains a novel junction between protein segments III-14 and I-11. We report production of a monoclonal antibody specific for (V+C)(-) fibronectin without cross-recognition of V(+)C(+) and V(-)C(+) isoforms found in plasma and other tissues. Presentation of epitope to this antibody requires the III-14/I-11 junction, but the epitope itself extends beyond 14 amino acids immediately surrounding the junction site and involves a conformational change in III-14 and/or the N-terminal portion of I-11. The antibody, designated Mab 5D10 anti (V+C)(-), displays specificity for (V+C)(-) fibronectin from multiple mammalian species including humans and utility in immunoblots, immunohistochemistry, and ELISA.
authors.affiliation: James A Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. niwl@cornell.edu
authors.forename: Nancy
authors.initials: N
authors.lastname: Burton-Wurster
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Rina
authors.initials: R
authors.lastname: Gendelman
authors.forename: Michael L
authors.initials: ML
authors.lastname: Jackson
authors.forename: Lucille F
authors.initials: LF
authors.lastname: Gagliardo
authors.forename: Da Nian
authors.initials: DN
authors.lastname: Gu
authors.forename: Russell R
authors.initials: RR
authors.lastname: Zelko
authors.forename: George
authors.initials: G
authors.lastname: Lust
authors.forename: James N
authors.initials: JN
authors.lastname: MacLeod
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002356:Cartilage / Q000737:chemistry; D003429:Cross Reactions; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007158:Immunologic Techniques; D020033:Protein Isoforms / Q000032:analysis; D013582:Synovial Fluid / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D005353:Fibronectins; D020033:Protein Isoforms