title: Independent translocation of two micronemal proteins in developing Plasmodium falciparum merozoites.
abstract: Apical membrane antigen 1 of Plasmodium falciparum (PfAMA1) contains an N-terminal propeptide that is removed prior to the translocation of the mature protein onto the merozoite surface. We localized unprocessed PfAMA1 to the microneme organelles of the intraerythrocytic schizont. The results have suggested that the processed form of PfAMA1 translocates from the microneme compartment independently of another microneme protein, EBA175, which is also involved in the invasion of human erythrocytes.
authors.affiliation: The Walter and Eliza Hall Institute of Medical Research. School of Botany, University of Melbourne, Melbourne, Australia.
authors.forename: Julie
authors.initials: J
authors.lastname: Healer
authors.forename: Simon
authors.initials: S
authors.lastname: Crawford
authors.forename: Stuart
authors.initials: S
authors.lastname: Ralph
authors.forename: Geoff
authors.initials: G
authors.lastname: McFadden
authors.forename: Alan F
authors.initials: AF
authors.lastname: Cowman
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D016778:Malaria, Falciparum / Q000209:etiology / Q000469:parasitology; D008565:Membrane Proteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D008954:Models, Biological; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D010963:Plasmodium falciparum / Q000254:growth & development* / Q000378:metabolism* / Q000472:pathogenicity / Q000648:ultrastructure; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D015800:Protozoan Proteins / Q000378:metabolism*; D014774:Virulence / Q000502:physiology
chemical_list: D000953:Antigens, Protozoan; D002352:Carrier Proteins; D008565:Membrane Proteins; D011498:Protein Precursors; D015800:Protozoan Proteins; C090077:apical membrane antigen I, Plasmodium; C065668:erythrocyte-binding antigen 175, Plasmodium; D010450:Endopeptidases