Protective effect of polypeptide from Chlamys farreri on hairless mice damaged by ultraviolet A.
OBJECTIVE
To study the protective effect of the polypeptide isolated from Chlamys farreri (PCF) on hairless mice skin damaged by ultraviolet A.


METHODS
Enzymes and malondialdehyde (MDA) were determined by biochemical methods; the expressions of Bcl-2 protein and NOS protein were examined by immunohistochemical technique. The ultra-structure of the skin was observed through electronic microscope.


RESULTS
PCF could enhance the activities of glutathione peroxidase (GSH-px), superoxide dismutase (SOD), and total anti-oxidative capacity (T-AOC). Also PCF could reduce the amount of MDA, increase the expression of Bcl-2 protein, and inhibit the expression of NOS protein. The ultra-structure of epidermis and fibroblasts remained normal in 20 % PCF groups; there were vacuoles in smooth endoplasm reticulum in epidermis of mice and the number of rough endoplasm reticulum in fibroblasts was decreased in model group.


CONCLUSIONS
PCF had the protective effects on hairless mice skin damaged by ultraviolet A via its anti-oxidative mechanisms.
Wang
Chun-Bo
CB
Medical College Qingdao University, Qingdao 266021, China.
Yao
Ru-Yong
RY
Liu
Zhan-Tao
ZT
Zhong
Wei-Zhen
WZ
Liu
Xiao-Ping
XP
Wang
Yue-Jun
YJ
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D051379:Mice; D008812:Mice, Hairless; D008974:Mollusca / Q000737:chemistry*; D010455:Peptides / Q000627:therapeutic use*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D012867:Skin / Q000528:radiation effects / Q000648:ultrastructure; D014466:Ultraviolet Rays
D000975:Antioxidants; D010455:Peptides; D019253:Proto-Oncogene Proteins c-bcl-2; D011837:Radiation-Protective Agents