Resonance CARS study of the structure of "green" and "red" chromophores within the red fluorescent protein DsRed.
Vibrational spectra of red fluorescent protein DsRed have been studied for the first time by polarization-sensitive multiplex coherent anti-Stokes Raman scattering at two excitation wavelengths, 545 and 583 nm, in resonance with the absorption bands of the immature "green" and mature "red" protein chromophores. Overall vibrational patterns of both DsRed chromophores were found to be similar to each other and to differ from that of S65T-GFP at pH8. The combined analysis of our CARS data and published structural information suggest that both "green" and "red" DsRed species possess an extended chromophore structure. Consequently, our data suggest that pi-bonding system extension during isomerization around the cis peptide bond between Phe 65 and Gln 66 is a necessary but not sufficient step in DsRed chromophore maturation.
Kruglik
Sergei G
SG
Department of Applied Physics, Biophysical Techniques Group, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Subramaniam
Vinod
V
Greve
Jan
J
Otto
Cees
C
D000818:Animals; D003063:Cnidaria / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods; D000097573:Red Fluorescent Protein
D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D000097573:Red Fluorescent Protein