title: [Two cases of mixed infection of malaria diagnosed by PCR method].
abstract: We here reported two Japanese cases of mixed infections of plasmodium species, whose DNAs were detected using the PCR test. One case was a 31 year-old male, who presented fever and fatigue, and had a travel history to Kenya, Cameroon and Indonesia. Smear test of his peripheral blood found the presence of Plasmodium vivax, while nested-PCR diagnosis detected the DNAs both P. vivax and Plasmodium malariae. The other was a 54 year-old female suffering from general fatigue. She had been treated with chloroquine for falciparum malaria in Indonesia two weeks before. Malaria antigen test showed positive although no Plasmodium organisms were found in the smear test. The nested PCR detected the DNA of Plasmodium ovale in addition to that of Plasmodium falciparum. In conclusion, the PCR test is helpful and useful for detection of mixed infections of Plasmodium species.
authors.affiliation: First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus.
authors.forename: Shusaku
authors.initials: S
authors.lastname: Haranaga
authors.forename: Manabu
authors.initials: M
authors.lastname: Akashi
authors.forename: Satomi
authors.initials: S
authors.lastname: Yara
authors.forename: Masaki
authors.initials: M
authors.lastname: Tohyama
authors.forename: Masato
authors.initials: M
authors.lastname: Toyama
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Ishimine
authors.forename: Takayuki
authors.initials: T
authors.lastname: Miyara
authors.forename: Takashi
authors.initials: T
authors.lastname: Shinzato
authors.forename: Futoshi
authors.initials: F
authors.lastname: Higa
authors.forename: Masao
authors.initials: M
authors.lastname: Tateyama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Saito
authors.forename: Hiromu
authors.initials: H
authors.lastname: Toma
mesh_terms: D000328:Adult; D000818:Animals; D005260:Female; D006801:Humans; D008288:Malaria / Q000150:complications* / Q000175:diagnosis*; D016778:Malaria, Falciparum / Q000175:diagnosis*; D016780:Malaria, Vivax / Q000150:complications* / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D010965:Plasmodium malariae; D016133:Polymerase Chain Reaction; D014195:Travel