Effects of garlic components diallyl sulfide and diallyl disulfide on arylamine N-acetyltransferase activity and 2-aminofluorene-DNA adducts in human promyelocytic leukemia cells.
Two components of garlic, diallyl sulfide (DAS) and diallyl disulfide (DADS), inhibited arylamine N-acetyltransferase (NAT) activity and 2-aminofluorene-DNA adduct in human promyelocytic leukemia cells (HL-60). The NAT activity was measured by high performance liquid chromatography assaying for amounts of N-acetyl-2-aminofluorene (2-AAF) and remaining 2-aminofluorene (2-AF). Cellular cytosols and intact cell suspensions were assayed. The inhibition of NAT activity and 2-AF-DNA adduct formation in human leukemia cells by DAS and DADS were dose-dependent and were directly proportional. The data also indicated that DAS and DADS decrease the apparent values of Km and Vmax from human leukemia cells in both assays. This is the first report of garlic components affecting human leukemia cell NAT activity and 2-AF-DNA adduct formation.
Lin
J G
JG
Institute of Chinese Medical Science, China Medical College, Taichung, Taiwan, ROC.
Chen
G W
GW
Su
C C
CC
Hung
C F
CF
Yang
C C
CC
Lee
J H
JH
Chung
J G
JG
D000498:Allyl Compounds / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D001191:Arylamine N-Acetyltransferase / Q000187:drug effects; D018736:DNA Adducts / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D004220:Disulfides / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D005449:Fluorenes / Q000737:chemistry / Q000378:metabolism; D005737:Garlic; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000517:prevention & control*; D008517:Phytotherapy; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D013440:Sulfides / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*
C052668:2-aminofluorene-DNA complex; D000498:Allyl Compounds; D016588:Anticarcinogenic Agents; D018736:DNA Adducts; D004220:Disulfides; D005449:Fluorenes; D010938:Plant Oils; D013440:Sulfides; C028009:diallyl disulfide; C038491:allyl sulfide; D001191:Arylamine N-Acetyltransferase