title: Antioxidant pathways in human aged skeletal muscle: relationship with the distribution of type II fibers.
abstract: Type II fiber loss and reactive oxygen species (ROS)-induced damage are hallmarks of muscle aging. The aim of this study was to analyze whether there exists a relationship between age-dependent changes in cellular antioxidant capacity and type II fiber loss in aged human skeletal muscles. Forty-five male and female subjects ranging in age from 65 to 90 year-old were divided into +40 and -40% type II fiber groups. We measured both total and Mn superoxide dismutase (total and MnSOD), glutathione peroxidase (GSHPx) and catalase (CAT) activities. We also measured the reduced and oxidized forms of glutathione (GSH and GSSG) and lipid peroxide (LPO) levels. Total SOD activity was lower in the -40% type II fiber group than in the +40% group; MnSOD tended to be lower but data are not statistically consistent. Both GSHPx and CAT activities remained unchanged; as did GSH, GSSG and GSH/GSSG ratio. Finally, muscle samples with -40% type II fibers had a significantly higher LPO content compared to those with +40% type II fibers. In summary, a relationship between human skeletal muscle aging, type II fiber loss and ROS reactions seems to exist.
authors.affiliation: Department of Physiological and Pharmacological Cellular and Molecular Sciences, Section of Pharmacology and Pharmacological Biotechnologies, University of Pavia, P.zza Botta 11, 27100 Pavia, Italy.
authors.forename: Orietta
authors.initials: O
authors.lastname: Pansarasa
authors.forename: Giorgio
authors.initials: G
authors.lastname: Felzani
authors.forename: Jacopo
authors.initials: J
authors.lastname: Vecchiet
authors.forename: Fulvio
authors.initials: F
authors.lastname: Marzatico
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000032:analysis; D006801:Humans; D008054:Lipid Peroxides / Q000032:analysis; D008297:Male; D008560:Membrane Fluidity; D018485:Muscle Fibers, Skeletal / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D008054:Lipid Peroxides; D013482:Superoxide Dismutase; D005978:Glutathione