title: New prevalence estimate of TT virus (TTV) infection in low- and high-risk population from São Paulo, Brazil.
abstract: The prevalence of TT virus (TTV) infection was investigated by Polymerase Chain Reaction (PCR) in low- (blood donors and healthy children/adolescents) and high-risk (hemophiliacs) groups from São Paulo, Brazil. Primers based on the untranslated region (UTR) of the viral genome proved to be much more ubiquitous, leading to much higher frequencies for both groups (>or= 81%) than the earlier N22-PCR directed to the open reading frame 1 (blood donors, 5.5%, and hemophiliacs, 42.3%). The UTR-PCR also revealed an interesting profile for healthy children/adolescents: very high prevalence at the early years and significant decrease in male teenagers. The N22-PCR, in turn, demonstrated higher frequency in hemophiliacs treated with fresh blood products (58%), than in those treated with virus-inactivated clotting factors (9.4%) and blood donors (5.5%).
authors.affiliation: Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, SP, Brazil.
authors.forename: Leda
authors.initials: L
authors.lastname: Bassit
authors.forename: Kioko
authors.initials: K
authors.lastname: Takei
authors.forename: Sumie
authors.initials: S
authors.lastname: Hoshino-Shimizu
authors.forename: Anna S
authors.initials: AS
authors.lastname: Nishiya
authors.forename: Ester C
authors.initials: EC
authors.lastname: Sabino
authors.forename: Rogério P
authors.initials: RP
authors.lastname: Bassitt
authors.forename: Roberto
authors.initials: R
authors.lastname: Focaccia
authors.forename: Elbio
authors.initials: E
authors.lastname: D'Amico
authors.forename: Dalton F
authors.initials: DF
authors.lastname: Chamone
authors.forename: Gabriela
authors.initials: G
authors.lastname: Ribeiro-Dos-Santos
mesh_terms: D000293:Adolescent; D001782:Blood Donors; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004266:DNA Virus Infections / Q000175:diagnosis / Q000453:epidemiology*; D005260:Female; D006467:Hemophilia A / Q000821:virology; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D016036:Seroepidemiologic Studies; D022783:Torque teno virus; D020506:Untranslated Regions
chemical_list: D020506:Untranslated Regions