Optimisation of a culture medium containing fish silage for L-lysine production by Corynebacterium glutamicum.
In a first step the effects of 10 components of a culture medium designed for L-lysine production were evaluated with a 2(10-6) factorial design. Among them, glucose, fish silage, and ammonium sulphate showed a significant effect. In a second step, an orthogonal-central composite experimental design and response surface methodology was performed with five from the 10 initial compounds. The determination coefficient (R2) of the fitted second-order model was 0.990. L-lysine production with the optimised medium increased 2.6 times as compared with the original medium.
Coello
Nereida
N
Laboratorio de Procesos Biotecnológicos, Instituto de Biologia Experimental, Universidad Central de Venezuela, Caracas. mcoello@reacciun.ve
Montiel
Edie
E
Concepcion
Milagros
M
Christen
Pierre
P
D000645:Ammonium Sulfate / Q000494:pharmacology*; D000818:Animals; D001709:Biotechnology / Q000379:methods; D003352:Corynebacterium / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology*; D005399:Fishes; D005947:Glucose / Q000494:pharmacology*; D007700:Kinetics; D008239:Lysine / Q000096:biosynthesis*; D013997:Time Factors
D003470:Culture Media; D005947:Glucose; D008239:Lysine; D000645:Ammonium Sulfate