Establishment of a primary effusion lymphoma cell line (RM-P1) and in vivo growth system using SCID mice.
Primary effusion lymphoma (PEL) is recognized as a unique lymphoma entity, which occurs exclusively in body cavities as a serous lymphomatous effusion without tumor formation or organ infiltration. We established a cell line of B-cell origin from a pericardial effusion of a 63-year-old Japanese PEL patient who did not have human immunodeficiency virus infection. This PEL cell line had human herpesvirus-8 (HHV-8) and Epstein-Barr virus (EBV) infection. We named this cell line RM-P1. This cell line showed complex chromosomal abnormalities that could not be identified by G-banding. However, spectral karyotyping analysis determined the origin and organization of all unidentified chromosomal abnormalities. When inoculated into the peritoneal cavity of 8 severe combined immunodeficiency (SCID) mice depleted of natural killer cells, RM-P1 cells induced solid tumor with ascites in all animals tested. These tumor and ascitic cells had the same immunogenotypic features as those of the original RM-P1. These 2 types of cells were positive for both HHV-8 and EBV as demonstrated using polymerase chain reaction. Fluorescence-activated cell sorting analyses showed that neither tumors nor ascitic cells grown in SCID mice expressed leukocyte function-associated antigen (LFA)-1alpha (CD11a), LFA-1lbeta (CD18), LFA-2 (CD2), LFA-3 (CD58), intercellular adhesion molecule (ICAM)-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), or leukocyte endothelial adhesion molecule (LECAM)-1 (CD62L), suggesting that these cytoadhesion molecules are not involved in tumor formation of RM-P1 cells in vivo. The establishment of the RM-P1 cell line and the animal model of PEL may provide insights for understanding the relationship between these viruses and PEL and for understand the mechanism for PEL.
Miyagi
Jun-ichi
J
Second Department of Internal Medicine, Faculty of Medicine, University of the Ryukus, Nishihara, Okinawa, Japan. mygj1@lime.ocn.ne.jp
Masuda
Masato
M
Takasu
Nobuyuki
N
Nagasaki
Akitoshi
A
Shinjyo
Tetsuharu
T
Uezato
Hiroshi
H
Kakazu
Naoki
N
Tanaka
Yuetsu
Y
D000818:Animals; D015815:Cell Adhesion Molecules / Q000032:analysis; D002455:Cell Division; D004854:Herpesvirus 4, Human; D019288:Herpesvirus 8, Human; D006801:Humans; D007621:Karyotyping; D008223:Lymphoma / Q000473:pathology* / Q000821:virology; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D009368:Neoplasm Transplantation; D016066:Pleural Effusion, Malignant / Q000473:pathology* / Q000821:virology; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000637:transplantation / Q000821:virology
D015815:Cell Adhesion Molecules