Stacking by electroinjection with discontinuous buffers in capillary zone electrophoresis.
The work presented here demonstrates that electroinjection can be performed using discontinuous buffers, which can result in better stacking than that obtained by hydrodynamic injection. The sample can be concentrated at the tip of the capillary leaving practically the whole capillary for sample separation. This results in several advantages, such as better sample concentration, higher plate number and shorter time of stacking. However, sample introduction by electromigration is suited for samples free or low in salt content. Samples, which are high in salt content, are better introduced by the hydrodynamic injection for stacking by the discontinuous buffers. Different simple methods to introduce the discontinuity in the buffer for electroinjection are discussed.
Shihabi
Zak K
ZK
Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. zshihabi@wfubmc.edu
D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D009647:Normetanephrine / Q000032:analysis; D012492:Salts; D014439:Tyramine / Q000032:analysis
D002021:Buffers; D012492:Salts; D009647:Normetanephrine; D014439:Tyramine