[Effects of Buyang huanwu decoction on the release of vWF and the expression of tissue factor induced by tumor necrosis factor alpha in cultured bovine aortic endothelial cells].
OBJECTIVE
To study the effects of Buyang huanwu decoction (BHD) on the release of von Willebrand factor(vWF) and the expression of tissue factor(TF) activity induced by tumor necrosis factor alpha (TNF alpha) in cultured bovine aortic endothelial cells (BAECs).


METHODS
BAECs from neonatal cox were cultured and 4-8 passages were used. Cells were incubated for 8 hours after addition of different treatments. The supernatant was used to measure vWF and BAECs lysate to determine TF activity.


RESULTS
1. Compared with the control, TNF alpha enhanced the expression of TF activity(12.79 +/- 2.59 vs. 4.69 +/- 0.83, P < 0.01) and the effects were in dose-dependent manner(r = 0.9712, P < 0.01); BHD inhibited the effects of TNF alpha(P < 0.01). 2. Compared with the control, TNF alpha promoted the release of vWF from endothelial cells (13.28 +/- 4.76 vs. 6.42 +/- 2.84, P < 0.01) and BHD inhibited the effect of TNF alpha(P < 0.01).


CONCLUSIONS
BHD can inhibit the expression of TF and the release of vWF induced by TNF alpha.
Shang
G P
GP
Department of Physiology, Hunan Medical University, Changsha 410078.
Wen
Z B
ZB
Li
J C
JC
D000818:Animals; D000831:Animals, Newborn; D001011:Aorta / Q000166:cytology; D002417:Cattle; D002478:Cells, Cultured; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D013925:Thromboplastin / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014841:von Willebrand Factor / Q000378:metabolism*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha; D014841:von Willebrand Factor; D013925:Thromboplastin