title: Transcriptional regulation of the human high affinity IgE receptor alpha-chain gene.
abstract: Transcriptional regulation of the gene encoding human high affinity IgE receptor (Fc epsilon RI) alpha-chain was analyzed. Previously, we reported that GATA-1 and Elf-1 recognition sites were necessary for cell type-specific activation of the alpha-chain gene promoter. More detailed analysis revealed that other transcription factors bound the regions close to the Elf-1 recognition site and there was a more complex mechanism for the regulation of the promoter activity. On the other hand, during a course of studies to find cis-elements over this gene, CAGCTG sequence in the first intron was revealed to serve as an enhancer. A complex composed of USF1 and USF2 activated the human alpha-chain gene expression via this intronic element. Furthermore, we found two novel exons at 18.4 and 12.6kb upstream from the reported first exon and discovered an additional distal promoter.
authors.affiliation: Department of Molecular Cell Immunology and Allergology, Advanced Medical Research Center, Nihon University School of Medicine, 30-1 Oyaguchi-kamimachi, Itabashi-ku, 173-8610, Tokyo, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Takahashi
authors.forename: Chiharu
authors.initials: C
authors.lastname: Nishiyama
authors.forename: Chisei
authors.initials: C
authors.lastname: Ra
mesh_terms: D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017455:Receptors, IgE / Q000096:biosynthesis / Q000235:genetics*; D015533:Transcriptional Activation
chemical_list: C475664:FCER1A protein, human; D017455:Receptors, IgE