title: Wheat (Triticum aestivum L.) [gamma]-Gliadin Accumulates in Dense Protein Bodies within the Endoplasmic Reticulum of Yeast.
abstract: Following their sequestration into the endoplasmic reticulum (ER), wheat storage proteins may either be retained and packaged into protein bodies within this organelle or transported via the Golgi to vacuoles. We attempted to study the processes of transport and packaging of wheat storage proteins using the heterologous expression system of yeast. A wild-type wheat [gamma]-gliadin, expressed in the yeast cells, accumulated mostly within the ER and was deposited in protein bodies with similar density to natural protein bodies from wheat endosperm. This suggested that wheat storage proteins contain sufficient information to initiate the formation of protein bodies in the ER of a heterologous system. Only a small amount of the [gamma]-gliadin was transported to the yeast vacuoles. When a deletion mutant of the [gamma]-gliadin, lacking the entire N-terminal repetitive region, was expressed in the yeast cells, the mutant was unable to initiate the formation of protein bodies within the ER and was completely transported to the yeast vacuole. This strongly indicated that the information for packaging into dense protein bodies within the ER resides in the N-terminal repetitive region of the [gamma]-gliadin. The advantage of using yeast to identify the signals and mechanisms controlling the transport of wheat storage proteins and their deposition in protein bodies is discussed.
authors.affiliation: Department of Plant Genetics (N.R., Y.S., Y.A., H.L., G.G.) and Department of Biochemistry (M.V.), The Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: N.
authors.initials: N
authors.lastname: Rosenberg
authors.forename: Y.
authors.initials: Y
authors.lastname: Shimoni
authors.forename: Y.
authors.initials: Y
authors.lastname: Altschuler
authors.forename: H.
authors.initials: H
authors.lastname: Levanony
authors.forename: M.
authors.initials: M
authors.lastname: Volokita
authors.forename: G.
authors.initials: G
authors.lastname: Galili