[Participation of dexamethasone and E and C vitamins in the modulation of the hepatotoxic effect induced by fomesafen and 2,4-D amino herbicides, in rats ].
The fomesafen and 2,4-D amine herbicide induce cytotoxic effects at hepatic level in rats, such as: hepatomegaly, hyperplasia and increase in the enzymes activity which participate in the processes of peroxisomal beta-oxidation of fatty acids. In this work, the effect of vitamin E and C was evaluated, as well as, the dexamethasone in the modulation of these hepatotoxic effects. Sprague-Dawley rats were treated with the herbicides and with the agents to be evaluated. The different treatments were given during 15 days orally route. The herbicides combined with the dexamethasone and antioxidant agents were administrated only and simultaneously with the herbicides. Once concluded the different treatment, the rats were weighed and sacrificed. It was evaluated the liver size and liver fragments were obtained to determine the enzymatic activity of Fatty Acyl CoA-oxidase (FACO) and cellular number. The results showed that the hepatomegaly induced by fomesafen was inhibited by the vitamins and by the dexamethasone, while any effect was not observed in the group of rats treated with 2,4-D amine. None of the agents modulated the FACO activity induced by herbicides in treated rats. However, the dexamethasone showed a protective effect in the hyperplasia induced by two herbicides. The hepatotoxic effects induced by the herbicides responded to a different mechanism due to the differences of the effects observed at the antioxidant agents. On the other hand, the inhibition of the cellular proliferation by the dexamethasone does not keep relation with the responsible mechanisms of inducing the oxidant stress into FACO activity. Under experimental conditions of this study, the use of these agents does not guarantee protection against the hepatotoxic effects induced by the herbicides.
Orfila
Luz
L
Instituto de Investigaciones Farmacéuticas, Unidad de Cultivo Celular-Toxicología, Facultad de Farmacia Universidad Central de Venezuela, Caracas-Venezuela.
Mendoza
Solangela
S
Rodríguez
Jesús
J
Arvelo
Francisco
F
D015084:2,4-Dichlorophenoxyacetic Acid / Q000037:antagonists & inhibitors* / Q000633:toxicity; D042962:Acyl-CoA Oxidase; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001549:Benzamides / Q000037:antagonists & inhibitors* / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury; D003907:Dexamethasone / Q000494:pharmacology*; D004123:Dimethylamines / Q000037:antagonists & inhibitors* / Q000633:toxicity; D006529:Hepatomegaly / Q000139:chemically induced; D006540:Herbicides / Q000037:antagonists & inhibitors* / Q000633:toxicity; D006965:Hyperplasia / Q000139:chemically induced; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D010088:Oxidoreductases / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology*
D000975:Antioxidants; D001549:Benzamides; D004123:Dimethylamines; D006540:Herbicides; D014815:Vitamins; D014810:Vitamin E; C037991:2,4-D amine; D015084:2,4-Dichlorophenoxyacetic Acid; D003907:Dexamethasone; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase; C088563:fomesafen; D001205:Ascorbic Acid