Protective effects of Ganoderma lucidum polysaccharides peptide on injury of macrophages induced by reactive oxygen species.
OBJECTIVE
To study the protective effects of Ganoderma lucidum polysaccharides peptide (GLPP) on the mice peritoneal macrophages injured by reactive oxygen species (ROS), derived from tert-butylhydroperoxide (tBOOH) in vitro and in vivo.


METHODS
Mice peritoneal macrophages were injured by ROS, derived from tBOOH. The survival rate of macrophages was measured by MTT assay, and the morphological changes of macrophages were observed under light and electron microscopes.


RESULTS
GLPP (50, 100, 200 mg/kg, ip for 5 d) could inhibit the foam cell formation and necrosis of macrophages. The survival rate of macrophages was increased. GLPP (3.125, 12.5, 50, 200 mg/L) given to the cultured macrophages brought the same protective effects. Under the electron microscope it was found that GLPP (100 mg/kg, ip, for 5 d) could protect the organelle such as mitochondria against injury by tBOOH.


CONCLUSIONS
GLPP had significant scavenging ROS and antioxidant effects.
You
Yu-Hong
YH
Department of Pharmacology, School of Basic Medical Science, Peking University Health Science Center, Beijing 100083, China.
Lin
Zhi-Bin
ZB
D000818:Animals; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011509:Proteoglycans / Q000302:isolation & purification / Q000494:pharmacology*; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D020940:Reishi / Q000737:chemistry*
D016166:Free Radical Scavengers; D011509:Proteoglycans; D017382:Reactive Oxygen Species; C093216:polysaccharide peptide