In vitro immunomodulatory activity of verbascoside from Nepeta ucrainica L.
Nepeta ucrainica L. is used as a herbal tea in Kazakhistan. Phytochemical investigations of the aerial parts of the plant resulted in the isolation of verbascoside (1) and 1,5,9-epi-deoxyloganic acid (2). The structures of the compounds were elucidated by spectral (UV, IR, (1)H-NMR and (13)C-NMR) methods and HPLC analysis. The in vitro immunomodulatory activity of verbascoside was investigated by assessing neutrophil function; chemotaxis and intracellular killing activity. Verbascoside showed an increased chemotactic activity in all doses applied compared with the medium used as a negative control and had a positive effect on respiratory burst of neutrophils, but there was an opposite effect with increasing doses, pointing to a possible suppression of neutrophil intracellular killing activity.
Akbay
Pinar
P
Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, 06100 Sihhiye, Ankara, Turkey.
Calis
Ihsan
I
Undeger
Ulkü
U
Basaran
Nursen
N
Basaran
A Ahmet
AA
D000276:Adjuvants, Immunologic / Q000097:blood / Q000494:pharmacology*; D000470:Alkaloids / Q000097:blood / Q000737:chemistry / Q000494:pharmacology; D001628:Beverages; D002633:Chemotaxis / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D057888:Iridoid Glucosides; D039823:Iridoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D027521:Nepeta; D009504:Neutrophils / Q000187:drug effects; D010636:Phenols / Q000097:blood / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet; D059808:Polyphenols
D000276:Adjuvants, Immunologic; D000470:Alkaloids; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D010636:Phenols; D010936:Plant Extracts; C468757:1,5,9-epi-deoxyloganic acid; C058956:acteoside; D059808:Polyphenols