Effects of phytohormones on the surfaces of plant-parasitic nematodes.
The direct effects of phytohormones (auxin and kinetin) and root diffusates on the surface lipophilicity of the plant parasitic nematodes Globodera rostochiensis and Meloidogyne incognita were investigated. The fluorescent lipid probe AF18 (5-N(octodecanoyl) aminofluorescein) was used to detect surface changes. Root diffusates increased AF18 uptake by G. rostochiensis while it had no effect on M. incognita. Kinetin and auxin decreased AF18 uptake in G. rostochiensis, while they had the opposite effect on M. incognita. Auxin/kinetin ratio was also found to be important in triggering the surface changes, especially at high concentrations. Whether plant nematodes have auxin and/or kinetin binding proteins is discussed as well as the mechanism behind the surface lipophilicity changes due to root diffusates and phytohormones.
Akhkha
A
A
Division of Biochemistry and Molecular Biology, University of Glasgow, UK. a.akhkha@bio.gla.ac.uk
Kusel
J
J
Kennedy
M
M
Curtis
R
R
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D007210:Indoleacetic Acids / Q000378:metabolism / Q000494:pharmacology; D007701:Kinetin; D050356:Lipid Metabolism; D018551:Solanum lycopersicum; D009348:Nematoda / Q000187:drug effects* / Q000378:metabolism*; D009349:Nematode Infections / Q000469:parasitology; D010935:Plant Diseases / Q000469:parasitology; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D010944:Plants / Q000469:parasitology*; D011493:Protein Kinase C / Q000378:metabolism; D011198:Solanum tuberosum; D014431:Tylenchoidea / Q000187:drug effects / Q000378:metabolism
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D011493:Protein Kinase C; D000225:Adenine; D007701:Kinetin