Carbon and nitrogen substrate utilization by archival Salmonella typhimurium LT2 cells.
BACKGROUND
A collection of over 20,000 Salmonella typhimurium LT2 mutants, sealed for four decades in agar stabs, is a unique resource for study of genetic and evolutionary changes. Previously, we reported extensive diversity among descendants including diversity in RpoS and catalase synthesis, diversity in genome size, protein content, and reversion from auxotrophy to prototrophy.


RESULTS
Extensive and variable losses and a few gains of catabolic functions were observed by this standardized method. Thus, 95 catabolic reactions were scored in each of three plates in wells containing specific carbon and nitrogen substrates.


CONCLUSIONS
While the phenotype microarray did not reveal a distinct pattern of mutation among the archival isolates, the data did confirm that various isolates have used multiple strategies to survive in the archival environment. Data from the MacConkey plates verified the changes in carbohydrate metabolism observed in the Biolog system.
Tracy
Breca S
BS
Stephens College, 1200 E. Broadway, Columbia, MO 65215, USA. brecastarr@hotmail.com
Edwards
Kelly K
KK
Eisenstark
Abraham
A
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D002374:Catalase / Q000096:biosynthesis; D019143:Evolution, Molecular; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D014644:Genetic Variation; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism*; D010641:Phenotype; D011309:Preservation, Biological; D012486:Salmonella typhimurium / Q000737:chemistry / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D012808:Sigma Factor / Q000235:genetics; D013534:Survival
D001426:Bacterial Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D002244:Carbon; D002374:Catalase; D009584:Nitrogen