Overexpression of TIAP/m-survivin in thymocytes enhances cell proliferation.
TIAP/m-survivin, a member of the inhibitor of apoptosis (IAP) protein family, is expressed in a cell cycle dependent manner. It is strongly expressed in various subsets of thymocytes. To investigate a role of TIAP/m-survivin in thymocytes, mice carrying the lck-TIAP transgene were established. Two out of six transgenic mice expressed large amounts of TIAP mRNA and protein in thymocytes. Although T cell development and apoptosis of thymocytes were largely unaffected in lck-TIAP mice, transgenic thymocytes displayed hyperproliferation in response to PMA and ionomycin but not to anti-CD3 antibody. Thus, overexpression of TIAP/m-survivin augments cell proliferation of thymocytes to a certain stimulation.
Hikita
Satosi
S
Department of Developmental Genetics (H2), Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
Hatano
Masahiko
M
Inoue
Atsushi
A
Sekita
Nobuyuki
N
Kobayashi
Koichi
K
Otaki
Masayuki
M
Ogasawara
Takeshi
T
Okada
Seiji
S
Hirasawa
Hiroyuki
H
Tokuhisa
Takeshi
T
D000818:Animals; D017209:Apoptosis; D005656:Fungal Proteins / Q000502:physiology; D051034:Inhibitor of Apoptosis Proteins; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D012097:Repressor Proteins / Q000502:physiology*; D000077022:Survivin; D013601:T-Lymphocytes / Q000502:physiology*
C117749:Birc5 protein, mouse; D005656:Fungal Proteins; D051034:Inhibitor of Apoptosis Proteins; D012097:Repressor Proteins; D000077022:Survivin