Studies on the Conformation of Apocytochrome c in Different Folding States Inserted into the Membranes.
Correlation between the folding states of the chicken heart apocytochrome c and its membrane insertion ability was studied by the monolayer experiment. The penetration ability of apocytochrome c decreased with its folding. Intrinsic fluorescence emissions of apocytochrome c interacted with soybean phospholipids liposomes suggested that the conformations of apocytochrome c with different folding states in aqueous solution were also different in the membranes. The conformational differences were further characterized by CD spectra of apocytochrome c interacted with DMPC and DMPG liposomes. The results showed that apocytochrome c, which were randomly coiled in aqueous solution, adopted alpha-helical conformations after interaction with DMPG liposome. The apocytochrome c with a folded state in aqueous solution also adopted alpha-helical conformations, but the alpha-helical contents were less than the former. When DMPC liposomes were used, no distinct conformational change was observed.
Han
Xue-Hai
XH
National Laboratory of Biomacromolecules, Institute of Biophysics Academia Sinica, Beijing 100101, China.
Sui
Sen-Fang
SF
Yang
Fu-Yu
FY