Cloning and analysis of CoEXG1, a secreted 1,3-beta-glucanase of the yeast biocontrol agent Candida oleophila.
Lytic enzymes may have a role in the biological control of fungi. The yeast biocontrol agent, Candida oleophila, is an excellent subject to research this matter. In the present study, CoEXG1, which encodes for a secreted 1,3-beta-glucanase, is the first gene to be cloned from C. oleophila. It was isolated from a partial genomic library and analysed. Its open reading frame and putative promoter were expressed in baker's yeast, Saccharomyces cerevisiae. The reading frame, expressed under the inducible GAL1 promoter, caused an increased secretion of beta-glucanase, and the putative promoter region activated the lacZ reporter gene, to which it was fused. Sequencing analysis revealed that CoEXG1 carries the signature pattern of the 5 glycohydrolases family and has a putative secretion leader, as well as a high degree of identity to yeast 1,3-beta-glucanases. The GenBank Accession No. of CoEXG1 is AF393806.
Segal
Efrat
E
Department of Molecular Genetics, MIGAL Galilee Technological Center, PO Box 90000, Rosh Pina 12100, Israel.
Yehuda
Hila
H
Droby
Samir
S
Wisniewski
Michael
M
Goldway
Martin
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D005659:Fungicides, Industrial; D043326:Glucan 1,3-beta-Glucosidase; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D011401:Promoter Regions, Genetic; D001617:beta-Glucosidase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D005659:Fungicides, Industrial; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase