Effects of copper on the expression of metal transporters in human intestinal Caco-2 cells.
Copper is an essential dietary trace metal, however the mechanisms involved in intestinal copper uptake are unclear. Two putative copper transporters are expressed in Caco-2 cells, the divalent metal transporter (DMT1) and copper transporter (Ctr1). Our data demonstrate that copper could compete with iron for uptake via DMT1 and that DMT1 protein and mRNA expression were decreased following exposure (24 h) to high copper. Expression of Ctr1, which acts as a copper transporter in transfected cell lines, was unaffected by copper treatment. Interestingly, exposure to copper increased iron efflux from Caco-2 cells and up regulated IREG1 (iron-regulated mRNA) expression.
Tennant
Jason
J
Centre for Nutrition and Food Safety, School of Biomedical and Life Sciences, University of Surrey, GU2 7XH, Guildford, UK. p.sharp@surrey.ac.uk
Stansfield
Matthew
M
Yamaji
Sachie
S
Srai
Surjit Kaila
SK
Sharp
Paul
P
D018938:Caco-2 Cells; D027682:Cation Transport Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology*; D000079923:Copper Transporter 1; D003470:Culture Media; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000378:metabolism; D033862:Iron-Binding Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020218:Response Elements / Q000187:drug effects; D000099236:Solute Carrier Family 11, Member 2
D027682:Cation Transport Proteins; D003300:Copper; D000079923:Copper Transporter 1; D003470:Culture Media; D007501:Iron; D033862:Iron-Binding Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D000099236:Solute Carrier Family 11, Member 2; C490079:SLC31A1 protein, human