High Expression in Insect Cells of A Functional Chimeric Antibody with Specificity for HBsAg.
We have constructed a double-recombinant virus BacHL4.2 containing both the chimeric heavy- and light-chain cDNAs from monoclonal antibody of the HBsAg gene. Both murine-human chimeric antibody heavy- and light-chain were expressed in Sf9 cells infected with a double-recombinant virus BacHL4.2 or co-infected with separate heavy- and light-chain recombinant viruses. In both cases, expressed products were correctly assembled into normal H(2)L(2) immunoglobulin monomers. ELISA and functional immunoblot assay showed that the recombinant chimeric antibody exhibited a specificity for HBsAg similar to that of the parental murine monoclonal antibody OH3.
Yu
Yong-Jun
YJ
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
Jiang
Yu-Lei
YL
Yang
Guan-Zhen
GZ
Li
Zai-Ping
ZP
Wu
Xiang-Fu
XF