Use of a highly sensitive quantitative telomerase assay in intracytoplasmic sperm injection programmes for the treatment of 47,XXY non-mosaic Klinefelter men.
We evaluated the role of the sensitive quantitative telomerase assay (SQTA) in the management of men with non-mosaic Klinefelter's syndrome (KS). Diagnostic testicular biopsy (DTB) was performed in 24 men with KS. A part of the DTB was stained and the remaining fragment was processed for the SQTA. After 3-18 months, a therapeutic testicular biopsy (TTB) was performed in the same testicle and the recovered specimens were processed to identify spermatozoa. Men with a SQTA outcome equal to 0.00 Units microg-1 protein (n = 7) demonstrated therapeutic testicular biopsy material that was negative for spermatogenic cells. In five men with a SQTA outcome of 8.11-38.03 Units microg-1, the most advanced germ cell was the spermatogonium/primary spermatocyte. In the remaining 12 men, the most advanced spermatogenic cell in the TTB was the spermatozoon. In these men, the SQTA outcome was equal to 25.76-92.68 Units microg-1 protein. Using 39.00 Units microg-1 protein as a cut-off value, the accuracy of the SQTA in identifying men positive for spermatozoa was 91.6%. It appears that the SQTA has a role for identifying non-mosaic KS men who have testicular spermatozoa.
Yamamoto
Y
Y
Tottori University School of Medicine, Tottori, Japan.
Sofikitis
N
N
Kaponis
A
A
Georgiou
J
J
Giannakis
D
D
Mamoulakis
Ch
Ch
Loutradis
D
D
Yiannakopoulos
X
X
Mio
Y
Y
Miyagawa
I
I
Chatzikyziakidou
A
A
D000328:Adult; D001706:Biopsy; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007713:Klinefelter Syndrome / Q000235:genetics* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009030:Mosaicism; D012680:Sensitivity and Specificity; D020554:Sperm Injections, Intracytoplasmic; D019098:Telomerase / Q000378:metabolism*; D013737:Testis / Q000473:pathology
D019098:Telomerase