Dose response relationship of disturbed migration of Purkinje cells in the cerebellum due to X-irradiation.
Pregnant rats were exposed to 2.0, 2.25 or 2.5 Gy X-irradiation on gestation day 21. Pups were sacrificed 12 hr after exposure, and on postnatal day 5 (P5), P7 and P9. Their cerebella were observed immunohistochemically using anti-inositol 1,4,5 triphosphate (IP3) receptor antibody to identify Purkinje cells. These cells were disturbed to migrate and remained in the internal granular layer and white matter of the cerebellum. They had short dendrites, and some showed an abnormal direction of dendrites in rats exposed to 2.25 or 2.5 Gy. Alignment of Purkinje cells was also disturbed when examined either on P5, P7 or P9 especially by doses of 2.25 and 2.5 Gy. There was a relationship between X-ray doses and the number of cells piling up in the Purkinje cell layer of the cerebellum. The dose-response relationship with the number of ectopic Purkinje cells was noted in the anterior lobes of the cerebellum.
Darmanto
W
W
Department of Teratology and Genetics, Division of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya University, Nagoya, Japan.
Inouye
M
M
Hayasaka
S
S
Takagishi
Y
Y
Aolad
H M
HM
Murata
Y
Y
D000818:Animals; D002465:Cell Movement / Q000528:radiation effects; D002531:Cerebellum / Q000166:cytology / Q000196:embryology / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005314:Embryonic and Fetal Development / Q000528:radiation effects; D005260:Female; D011247:Pregnancy; D011689:Purkinje Cells / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D014965:X-Rays