Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration.
The molecular mechanisms underlying lymphocyte extravasation remain poorly characterized. We have recently identified junctional adhesion molecule-2 (JAM-2), and have shown that antibodies to JAM-2 stain high endothelial venules (HEVs) within lymph nodes and Peyer patches of adult mice. Here we show that mouse lymphocytes migrate in greater numbers across monolayers of endothelioma cells transfected with JAM-2. The significance of these findings to an understanding of both normal and pathologic lymphocyte extravasation prompted us to clone the human homologue of JAM-2. We herein demonstrate that an anti-JAM-2 antibody, or a soluble JAM-2 molecule, blocks the transmigration of primary human peripheral blood leukocytes across human umbilical vein endothelial cells expressing endogenous JAM-2. Furthermore, we show that JAM-2 is expressed on HEVs in human tonsil and on a subset of human leukocytes, suggesting that JAM-2 plays a central role in the regulation of transendothelial migration.
Johnson-Léger
Caroline A
CA
Department of Pathology, University Medical Centre, 1, Rue Michel-Servet, Geneva, Switzerland. caroline.johnson-leger@medecine.unige.ch
Aurrand-Lions
Michel
M
Beltraminelli
Nicola
N
Fasel
Nicolas
N
Imhof
Beat A
BA
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D004730:Endothelium, Vascular / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D007136:Immunoglobulins / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D008214:Lymphocytes / Q000502:physiology*; D008565:Membrane Proteins / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D062765:Junctional Adhesion Molecule B
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D008565:Membrane Proteins; C411887:JAM2 protein, human; D062765:Junctional Adhesion Molecule B; C498781:Jam2 protein, mouse