Synthesis of a rabbit polyclonal antibody to the human sodium-dependent multivitamin transporter.
In mammalian cells, biotin is covalently attached to carboxylases and histones and is required for cell proliferation and function. Cellular uptake of biotin (as well as pantothenic acid and lipoic acid) is mediated by the sodium-dependent multivitamin transporter, SMVT. Studies of cellular biotin homeostasis have been hampered by the lack of an antibody to SMVT. Here, we describe the synthesis of a rabbit polyclonal antibody to human SMVT. Using this antibody, SMVT has been identified in human peripheral blood mononuclear cells, Caco-2 cells, and HepG2 cells. Moreover, we observed that cells respond to proliferation with increased synthesis of SMVT.
Griffin
Jacob B
JB
Department of Nutritional Sciences and Dietetics, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583-0806, USA.
Stanley
J Steven
JS
Zempleni
Janos
J
D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D001710:Biotin / Q000378:metabolism*; D015153:Blotting, Western; D018938:Caco-2 Cells; D002352:Carrier Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D006706:Homeostasis; D006801:Humans; D007106:Immune Sera / Q000096:biosynthesis / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D010205:Pantothenic Acid / Q000378:metabolism; D011817:Rabbits; D027981:Symporters; D008063:Thioctic Acid / Q000378:metabolism
D000906:Antibodies; D002352:Carrier Proteins; D007106:Immune Sera; D008562:Membrane Glycoproteins; D027981:Symporters; C119981:biotin transporter; D010205:Pantothenic Acid; D001710:Biotin; D008063:Thioctic Acid