title: Linear dichroism for the detection of single base pair mutations.
abstract: Flow linear dichroism is shown to be able to detect single base mismatches in a polymerase chain reaction (PCR) amplimers from exon 10 of the human beta-glucocerebrosidase gene (associated with Gaucher disease) over a kilobase long with no post PCR manipulation.
authors.affiliation: Department of Clinical Biochemistry, Addenbrooke's Hospital, Hills Rd, Cambridge, UK CB2 2XY.
authors.forename: D J
authors.initials: DJ
authors.lastname: Halsall
authors.forename: A
authors.initials: A
authors.lastname: Rodger
authors.forename: T R
authors.initials: TR
authors.lastname: Dafforn
mesh_terms: D000483:Alleles; D020137:Base Pair Mismatch; D005091:Exons / Q000235:genetics; D005776:Gaucher Disease / Q000235:genetics*; D005962:Glucosylceramidase / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D013057:Spectrum Analysis
chemical_list: D005962:Glucosylceramidase