title: Biphasic Stimulation of Translational Activity Correlates with Induction of Translation Elongation Factor 1 Subunit [alpha] upon Wounding in Potato Tubers.
abstract: Potato (Solanum tuberosum) tubers exhibit an increase in translational activity in response to mechanical wounding. The response is biphasic, with an initial stimulation apparent within the first 2 h after wounding and a second increase occurring 12 to 24 h after wounding. Increased activity is apparent by measurement of protein synthesis both in vivo and in vitro using a cell-free extract. Accumulation of the translational elongation factor 1 subunit [alpha] (EF-1[alpha]) parallels translational activity. Changes in the steady-state level of EF-1[alpha] mRNA, and expression of a chimeric EF-1[alpha] promoter/[beta]-glucuronidase construct in transgenic potato tubers, indicate that the gene encoding EF-1[alpha] is transcribed during both periods of translational stimulation. These results indicate that stimulation of translational activity is coordinated with increased expression and accumulation of translation factors.
authors.affiliation: Department of Biochemistry, Microbiology and Molecular Biology, University of Maine, Orono, Maine 04469-5735 (J.K.M, M.E.V).
authors.forename: J. K.
authors.initials: JK
authors.lastname: Morelli
authors.forename: C. K.
authors.initials: CK
authors.lastname: Shewmaker
authors.forename: M. E.
authors.initials: ME
authors.lastname: Vayda