title: Liquid chromatographic resolution of gemifloxacin mesylate on a chiral stationary phase derived from crown ether.
abstract: A new racemic fluoroquinolone antibacterial agent, gemifloxacin mesylate, has been successfully resolved on a chiral stationary phase (CSP) derived from (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid. Compared to the Crownpak CR(+) column, the CSP used in this study was more effective for the resolution of racemic gemifloxacin mesylate, especially in terms of analytical time. The resolution of gemifloxacin mesylate enantiomers on the CSP was found to be dependent on the type and content of organic and acidic modifiers in the aqueous mobile phase and the column temperature.
authors.affiliation: Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Pusan 609-735, Korea. mhhyun@pusan.ac.kr
authors.forename: Myung Ho
authors.initials: MH
authors.lastname: Hyun
authors.forename: Sang Cheol
authors.initials: SC
authors.lastname: Han
authors.forename: Yoon Jae
authors.initials: YJ
authors.lastname: Cho
authors.forename: Jong Sung
authors.initials: JS
authors.lastname: Jin
authors.forename: Wonjae
authors.initials: W
authors.lastname: Lee
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004988:Ethers, Cyclic / Q000737:chemistry*; D024841:Fluoroquinolones; D000077735:Gemifloxacin; D009287:Naphthyridines / Q000032:analysis*; D013237:Stereoisomerism
chemical_list: D000890:Anti-Infective Agents; D004988:Ethers, Cyclic; D024841:Fluoroquinolones; D009287:Naphthyridines; D000077735:Gemifloxacin