A method to detect major serotypes of foot-and-mouth disease virus.
Nucleic acid sequence-based amplification (NASBA) is an isothermal technique that allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A rapid and specific NASBA technique was developed, allowing the detection of foot-and-mouth disease virus genetic material in a range of sample material, including preserved skin biopsy material from infected animals, vaccines prepared from denatured cell-free material, and cell-free antigen-based detection kits. A single pair of DNA oligonucleotide primers was able to amplify examples of all major FMD virus subtypes. The amplified viral RNA was detected by electrochemiluminescence. The method was at least as sensitive as existing cell-free antigen detection methods.
Collins
Richard A
RA
Hong Kong DNA Chips Ltd, 1805-6, 18/F, Lu Plaza, Kowloon, Hong Kong SAR, China.
Ko
Lung-Sang
LS
Fung
King Yip
KY
Lau
Lok-Ting
LT
Xing
Jun
J
Yu
Albert C H
AC
D000818:Animals; D002417:Cattle; D017931:DNA Primers / Q000235:genetics / Q000378:metabolism; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000276:immunology; D008163:Luminescent Measurements; D011189:Potassium Chloride / Q000378:metabolism; D021161:Self-Sustained Sequence Replication / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping
D017931:DNA Primers; D011189:Potassium Chloride