title: Screening approach for chiral separation of pharmaceuticals part II. Reversed-phase liquid chromatography.
abstract: A screening strategy for the rapid separation of drug enantiomers by reversed-phase liquid chromatography was developed using three cellulose/amylose stationary phases. The key point to achieve enantioselectivity is the control of the compound ionisation. Only two mobile phases, i.e. an acidic phosphate buffer (pH 2.0) containing a chaotropic salt (KPF6) and a borate buffer (pH 9.0) mixed with acetonitrile, are used in the proposed strategy. This strategy was successfully applied to a set of 37 diverse chiral pharmaceuticals. Satisfactory enantioselectivity was achieved for 89% of them.
authors.affiliation: Department of Pharmaceutical and Biomedical Analysis, Pharmaceutical Institute, Vrije Universiteit Brussel, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Perrin
authors.forename: N
authors.initials: N
authors.lastname: Matthijs
authors.forename: D
authors.initials: D
authors.lastname: Mangelings
authors.forename: C
authors.initials: C
authors.lastname: Granier-Loyaux
authors.forename: M
authors.initials: M
authors.lastname: Maftouh
authors.forename: D L
authors.initials: DL
authors.lastname: Massart
authors.forename: Y Vander
authors.initials: YV
authors.lastname: Heyden
mesh_terms: D000143:Acids; D002021:Buffers; D002853:Chromatography, Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents; D013237:Stereoisomerism
chemical_list: D000143:Acids; D002021:Buffers; D004364:Pharmaceutical Preparations; D012997:Solvents