[The RYR1 genotype of the pigs from Neijiang of China].
The aim of this study was to test the RYR1 genotype of 25 Neijiang pigs ao as to provide data for the selective breeding of medical experimental pig strains. PCR-RFLP technique was used. The results showed that the RYR1 genotype of all the 25 porcine cases was HALNN and all of the 25 porcine cases were Halothane Negative. These suggested that Neijiang pig could be one of the porcine strains selectively bred for medical experiments and clinically for pig-to-human xenotransplantation. PCR-RFLP technique is a reliable and simple method for RYR1 gene testing.
Chen
Y
Y
Lab of Transplant Immunology, First Affiliated Hospital, WCUMS, Chengdu 610041.
Bu
H
H
Li
Y
Y
Liu
J
J
Li
S
S
He
Q
Q
D000818:Animals; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics*; D013552:Swine; D014183:Transplantation, Heterologous
D019837:Ryanodine Receptor Calcium Release Channel