title: Gene expression in epithelial ovarian carcinoma.
abstract: We analysed the mRNA levels corresponding to 12,600 transcripts in primary cultures of ovarian epithelial cells derived from nine normal ovaries and 21 epithelial ovarian carcinoma. The class distinction and hierarchical clustering of expression data revealed a clear distinction in gene expression between normal and carcinoma-derived ovarian epithelial cells. Comparison of expression levels revealed 111 genes with mean expression values of >2.5-fold higher in carcinoma cells. Similarly, 62 genes were expressed at >2.5-fold higher levels in normal ovarian epithelial cells. For a few selected genes, we demonstrate that the pattern of differential expression observed in cultured epithelial cells is present in the normal ovaries and epithelial ovarian carcinoma. Use of cultured epithelial cells represents a novel strategy to study gene expression in a cell-type specific manner.
authors.affiliation: Department of Medicine, UCLA School of Medicine, 10833 Le Conte Avenue, Los Angeles, California, CA 90095, USA.
authors.forename: Daniela
authors.initials: D
authors.lastname: Matei
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Graeber
authors.forename: Rae Lynn
authors.initials: RL
authors.lastname: Baldwin
authors.forename: Beth Y
authors.initials: BY
authors.lastname: Karlan
authors.forename: Jianyu
authors.initials: J
authors.lastname: Rao
authors.forename: David D
authors.initials: DD
authors.lastname: Chang
mesh_terms: D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction