title: Interaction between malnutrition and ovarian hormones on the systemic IGF-I axis.
abstract: OBJECTIVE
  In malnutrition both the GH-IGF and reproductive axes are greatly affected. Because the actions of IGF and sex steroids are inter-dependent in many tissues, we have examined how ovariectomy modulates the response of the systemic IGF system to undernutrition.
  
  
  METHODS
  Peripubertal (30 days of age) female rats were either sham operated or ovariectomized. Five days later half of each group was submitted to a protein-caloric restriction diet. All rats were killed at 60 days of age.
  
  
  RESULTS
  Growth was decreased in all rats submitted to calorie restriction and this was consistent with a decrease in circulating IGF-I concentrations and liver IGF-I mRNA expression. While in normally fed rats ovariectomy had no significant effect on serum IGF-I concentrations, ovariectomized and underfed rats had significantly higher levels than intact underfed rats. In undernourished rats, serum IGF-binding proteins (IGFBP)-1, -2 and -3 concentrations were significantly reduced and this was not modified by ovariectomy. In contrast, liver mRNA concentrations of IGFBP-1 and -2 were increased and IGFBP-3 unchanged in intact undernourished animals, suggesting that undernutrition could be affecting the proteolysis of these binding proteins, and this response was significantly modulated by ovariectomy.
  
  
  CONCLUSIONS
  These results indicate that the presence of circulating ovarian hormones significantly affects the response of the IGF system to undernutrition.
authors.affiliation: Institute of Biochemistry, CSIC-Universidad Complutense, Madrid, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Goya
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: García-Segura
authors.forename: Sonia
authors.initials: S
authors.lastname: Ramos
authors.forename: Ana María
authors.initials: AM
authors.lastname: Pascual-Leone
authors.forename: Jesús
authors.initials: J
authors.lastname: Argente
authors.forename: María Angeles
authors.initials: MA
authors.lastname: Martín
authors.forename: Julie A
authors.initials: JA
authors.lastname: Chowen
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001835:Body Weight; D004044:Dietary Proteins / Q000008:administration & dosage; D002149:Energy Intake; D005260:Female; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000097:blood / Q000235:genetics; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000097:blood / Q000235:genetics; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000097:blood / Q000235:genetics; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000502:physiology*; D008099:Liver / Q000737:chemistry; D009748:Nutrition Disorders / Q000503:physiopathology*; D010052:Ovariectomy; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012260:Ribonucleases
chemical_list: D004044:Dietary Proteins; D018970:Insulin-Like Growth Factor Binding Protein 1; D018971:Insulin-Like Growth Factor Binding Protein 2; D018972:Insulin-Like Growth Factor Binding Protein 3; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D012260:Ribonucleases