Secondary structure of DNA is recognized by slightly cross-linked cationic hydrogel.
Interaction of salmon sperm DNA (300-500 bp) and ultrahigh molecular mass DNA (166 kbp) from bacteriophage T4dC with linear poly(N-diallyl-N-dimethylammonium chloride) (PDADMAC) and slightly cross-linked (#) PDADMAC (#PDADMAC) hydrogel in water has been studied by means of UV-spectroscopy, ultracentrifugation, atomic force, and fluorescence microscopy (FM). It is found that the linear polycation induced compaction of either native (double-stranded) or denatured (single-stranded) DNA by forming PDADMAC-DNA interpolyelectrolyte complexes (IPEC)s. At the same time, #PDADMAC hydrogel is able to distinguish between native and denatured DNA. Native DNA is adsorbed and captured in the hydrogel surface layer, while denatured DNA diffuses to the hydrogel interior until the whole hydrogel sample is transformed into the cross-linked IPEC. Both native and denatured DNA can be completely released from the hydrogel in appropriate conditions with no degradation by adding a low molecular salt. The data observed using conventional physicochemical methods with respect to DNA of a moderate molecular mass remarkably correlate with the pictures directly observed for ultrahigh molecular mass DNA in dynamics by using FM.
Sergeyev
Vladimir G
VG
Chemistry Department, Polymer Science Division, Moscow State University, Moscow, 119899 Russia. sergeyev@genebee.msu.su
Novoskoltseva
Olga A
OA
Pyshkina
Olga A
OA
Zinchenko
Anatoly A
AA
Rogacheva
Valentina B
VB
Zezin
Alexander B
AB
Yoshikawa
Kenichi
K
Kabanov
Victor A
VA
D000818:Animals; D017122:Bacteriophage T4 / Q000737:chemistry / Q000235:genetics; D002412:Cations; D003432:Cross-Linking Reagents / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008297:Male; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D009690:Nucleic Acid Conformation; D011095:Polyethylenes / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012474:Salmon / Q000235:genetics; D013056:Spectrophotometry, Ultraviolet; D013094:Spermatozoa / Q000737:chemistry
D002412:Cations; D003432:Cross-Linking Reagents; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D020100:Hydrogels; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D004247:DNA