Comparison of gene expression at the feto-maternal interface between normal and recurrent pregnancy loss patients.
Normal pregnancy requires a series of immunological, metabolic, vascular and endocrine regulating processes. However, the specific genes and proteins involved in these processes are not well defined. Aberration of these processes may lead to problems in pregnancy. One of these problems may be recurrent pregnancy loss (RPL). Little information is available on the level of expression of genes that may play a role in normal pregnancy. Therefore, this study determined whether different levels of gene expression at the feto-maternal interface could be associated with factors for RPL. The expression patterns of genes isolated from subtractive hybridization analysis performed with chorionic villi from normal and abnormal pregnancies were investigated. Eight genes classified into groups, including immunosuppression-related, embryo attachment-related and angiogenesis-related, were isolated.
Baek
Kwang-Hyun
KH
College of Medicine, Pochon CHA University, Infertility Medical Center, CHA General Hospital, Kangnam-Gu, Seoul, Korea. baek@cha.ac.kr
Choi
Bum Chae
BC
Lee
Jin-Hie
JH
Choi
Hee-Kyung
HK
Lee
Sook-Hwan
SH
Kim
Jung-Wook
JW
Hill
Joseph A
JA
Chung
Hyung-Min
HM
Ko
Jung Jae
JJ
Cha
Kwang Yul
KY
D000026:Abortion, Habitual / Q000235:genetics*; D006063:Chorionic Gonadotropin / Q000235:genetics; D002824:Chorionic Villi / Q000737:chemistry; D010064:Embryo Implantation / Q000235:genetics; D005260:Female; D005353:Fibronectins / Q000235:genetics; D015870:Gene Expression; D005914:Globins / Q000235:genetics; D000071816:Glycodelin; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D007108:Immune Tolerance / Q000235:genetics; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D018396:Mucin-1 / Q000235:genetics; D018919:Neovascularization, Physiologic / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods; D010446:Peptide Fragments / Q000235:genetics; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D006063:Chorionic Gonadotropin; D005353:Fibronectins; D000071816:Glycodelin; D006023:Glycoproteins; C116055:MUC1 tandem repeat peptide; D018396:Mucin-1; C045246:PAEP protein, human; D010446:Peptide Fragments; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D005914:Globins; D020778:Matrix Metalloproteinase 2