title: Sequence of the 3'-terminal end (8.1 kb) of the genome of porcine haemagglutinating encephalomyelitis virus: comparison with other haemagglutinating coronaviruses.
abstract: A cytopathogenic coronavirus, serologically identified as porcine haemagglutinating encephalomyelitis virus (HEV), has recently been associated with acute outbreaks of wasting and encephalitis in nursing piglets from pig farms in southern Québec and Ontario, Canada. The 3'-terminal end of the genome of the prototype HEV-67N strain and that of the recent Québec IAF-404 field isolate, both propagated in HRT-18 cells, were sequenced. Overall, sequencing data indicated that HEV has remained antigenically and genetically stable since its first isolation in North America in 1962. Compared with the prototype strain of bovine enteropathogenic coronavirus (BCoV), HEV, as well as the human respiratory coronavirus (HCoV-OC43) showed a major deletion in their ORF4 gene. Deduced amino acid sequences for both HEV strains revealed 89/88, 80, 93/92 and 95/94% identities with the structural proteins HE, S, M and N of BCoV and HCoV-OC43, respectively. Major variations were observed in the S1 portion of the S gene of both HEV strains, with only 73/71% amino acid identities compared with those of the two other haemagglutinating coronaviruses.
authors.affiliation: INRS-Institut Armand-Frappier, Centre de Microbiologie et Biotechnologie, Université du Québec, 531 boul. des Prairies, Laval, Québec, Canada H7V 1B71.
authors.forename: A Marie-Josée
authors.initials: AM
authors.lastname: Sasseville
authors.affiliation: INRS-Institut Armand-Frappier, Centre de Microbiologie et Biotechnologie, Université du Québec, 531 boul. des Prairies, Laval, Québec, Canada H7V 1B71.
authors.forename: Martine
authors.initials: M
authors.lastname: Boutin
authors.affiliation: INRS-Institut Armand-Frappier, Centre de Microbiologie et Biotechnologie, Université du Québec, 531 boul. des Prairies, Laval, Québec, Canada H7V 1B71.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Gélinas
authors.affiliation: INRS-Institut Armand-Frappier, Centre de Microbiologie et Biotechnologie, Université du Québec, 531 boul. des Prairies, Laval, Québec, Canada H7V 1B71.
authors.forename: Serge
authors.initials: S
authors.lastname: Dea
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D017934:Coronavirus / Q000235:genetics* / Q000302:isolation & purification; D018352:Coronavirus Infections / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine; D017361:Viral Nonstructural Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins