Spin trapping study of reactive oxygen species formation during bopindolol peroxidation.
The generation of singlet molecular oxygen ((1)O(2)) and hydroxyl radicals (HO*) during peroxidation of bopindolol in the presence of Co(II) ions was studied using electron spin resonance (ESR) and spectrophotometry methods. 2,2,6,6-Tetramethyl-4-piperidone and 5,5-dimethyl-1-pyrroline-1-oxide were used as traps. The spectrophotometry determination of (1)O(2) was based on bleaching of p-nitrosodimethylaniline (RNO), which was caused by the product of the reaction of (1)O(2) with imidazole and was followed by monitoring the decrease in optical density at 440 nm. The effect of (1)O(2) quenchers and oxygen free radical scavengers on the ESR signal and the bleaching of RNO was studied. The data presented here give new evidence for generation of the reactive oxygen species during peroxidation of bopindolol.
Kruk
Irena
I
Institute of Physics, Technical University of Szczecin, Al. Piastów 48/49, 70-310 Szczecin, Poland.
Michalska
Teresa
T
Kladna
Aleksandra
A
Aboul-Enein
Hassan Y
HY
D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals / Q000138:chemical synthesis; D009603:Nitroso Compounds / Q000737:chemistry; D010084:Oxidation-Reduction; D010869:Pindolol / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000138:chemical synthesis* / Q000737:chemistry; D013053:Spectrophotometry; D013113:Spin Labels; D019097:Spin Trapping; D013997:Time Factors
D016166:Free Radical Scavengers; D005609:Free Radicals; D009603:Nitroso Compounds; D017382:Reactive Oxygen Species; D013113:Spin Labels; C008651:4-nitrosodimethylaniline; D010869:Pindolol; C036007:bopindolol