title: Analysis of oligoguluronic acids with NMR, electrospray ionization-mass spectrometry and high-performance anion-exchange chromatography.
abstract: Oligoguluronates were prepared by enzymatic hydrolysis of homopolymeric blocks of guluronic acids. Two different oligosaccharides were prepared by separating final hydrolysates on Q-Sepharose FF ion chromatography. High-performance anion-exchange chromatography analysis showed the high purity of these oligosaccharides. The molecular masses of these two oligosaccharides, determined by electrospray ionization-mass spectrometry, were 396 and 594, respectively. 1H, 13C NMR, H-H COSY and HSQC analysis proved that they were the unsaturated dimer and trimer oligoguluronates. The 1H and 13C NMR chemical shifts of these two oligosaccharides are also reported.
authors.affiliation: Food Science and Technology, Fishery College, Ocean University of Qingdao, China. yanliu@mail.wsu.edu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiao-Lu
authors.initials: XL
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Liao
authors.forename: Hua-Shi
authors.initials: HS
authors.lastname: Guan
mesh_terms: D000837:Anion Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006603:Hexuronic Acids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000837:Anion Exchange Resins; D006603:Hexuronic Acids; C007896:guluronic acid