title: Use of selective medium for Burkholderia cepacia isolation in respiratory samples from cystic fibrosis patients.
abstract: Burkholderia cepacia colonizes cystic fibrosis (CF) patients. We evaluated the impact of the use of a selective medium in the rate of B. cepacia recovery from respiratory samples of CF patients. During a 6-month period, respiratory samples were collected from 106 CF patients and cultivated on selective media including a B. cepacia selective medium. Confirmation of the identity of B. cepacia isolates was carried out by species specific PCR and determination of genomovar status performed by a sequential PCR approach. Results of B. cepacia isolation during this period were compared to the preceding two years, when the sample processing was identical except for the lack of the B. cepacia selective medium. B. cepacia was isolated in 11/257 (4.2%) of the samples using the selective medium, in contrast with the preceding two years, when it was isolated in 6/1029 samples (0.58%), p < 0.0001. Identity of all 11 isolates was confirmed by PCR and genomovar determination was accomplished in all but one isolate. These results suggest that the use of a selective medium increases recovery rate of B. cepacia from respiratory samples.
authors.affiliation: Instituto da Criança Prof. Pedro de Alcântara, Hospital das Clínicas, University of São Paulo Medical School, São Paulo, Brazil. vicres@usp.br
authors.forename: Luiz V F
authors.initials: LV
authors.lastname: da Silva Filho
authors.forename: Luciana de F
authors.initials: Lde F
authors.lastname: Velloso
authors.forename: Christina N O
authors.initials: CN
authors.lastname: Bento
authors.forename: Edelyn
authors.initials: E
authors.lastname: Gytin
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Tateno
authors.forename: José E
authors.initials: JE
authors.lastname: Levi
authors.forename: Joaquim C
authors.initials: JC
authors.lastname: Rodrigues
authors.forename: Sonia R T S
authors.initials: SR
authors.lastname: Ramos
mesh_terms: D000293:Adolescent; D000328:Adult; D016956:Burkholderia cepacia / Q000235:genetics / Q000302:isolation & purification*; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D003550:Cystic Fibrosis / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D013183:Sputum / Q000382:microbiology*; D013997:Time Factors
chemical_list: D003470:Culture Media