title: Surface engineering of poly(D,L-lactic acid) by entrapment of chitosan-based derivatives for the promotion of chondrogenesis.
abstract: Chitosan and chitosan-amino acid derivatives were explored to engineer poly(D,L-lactic acid) (PDL-LA) as an extracellular matrix-like surface to promote cell adhesion and growth. Four kinds of chitosan-amino acid derivatives were prepared to mimic the carbohydrate moieties of cell matrix glycoprotein. The chitosan-amino acid derivatives were characterized by using Fourier transform infrared and ultraviolet spectra. The amino acid content on chitosan-amino acid derivatives was determined by using a ninhydrin-ultraviolet method. A new strategy, entrapment, was therefore used to modify the PDL-LA membrane with chitosan and chitosan-amino acid derivatives. The results of X-ray photoelectron spectroscopy, attenuated total reflectance-Fourier transform infrared, and contact angle confirmed that a stable thin film of chitosan and its derivatives can be entrapped on the surface of the PDL-LA membrane. From the results of chondrocyte cytocompatibility, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assays, and cell morphology, the chitosan-amino acid derivative modified PDL-LA membranes were shown to promote chondrogenesis. The novel surface treatment method combines the good mechanical property of PDL-LA with the good cytocompatibility of chitosan derivatives, which may have potential for tissue engineering.
authors.affiliation: Department of Polymer Science, Zhejiang University, Hangzhou 310027, China. jianji@mail.hz.zj.cn
authors.forename: Huiguang
authors.initials: H
authors.lastname: Zhu
authors.forename: Jian
authors.initials: J
authors.lastname: Ji
authors.forename: Rongyi
authors.initials: R
authors.lastname: Lin
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
authors.forename: Linxian
authors.initials: L
authors.lastname: Feng
authors.forename: Jiacong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D048429:Cell Size; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism; D048271:Chitosan; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D020219:Chondrogenesis / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000596:Amino Acids; D001672:Biocompatible Materials; D011091:Polyesters; D002686:Chitin; C033616:poly(lactide); D048271:Chitosan