[Study on the biosynthesis of para-aminobenzoic acid by S. sanguis. I. Establishment of a reversed-phase high-performance liquid chromatography].
In order to study the synthesis of para-aminobenzoic acid (PABA) by S. mutans and the role of PABA in the interaction between S. mutans and S. sanguis, a reversed-phase high-performance liquid chromatography (RP-HPLC) for analysis of PABA was developed. The results showed that the optimal chromatographic parameters were flow rate of 1.5 ml/min, T = 55 +/- 2 degrees C, methanol/phosphate buffer of 10/90(by vol) (pH 2.2 0.1 mol/L), m-hydroxybenzoic acid as an internal standard. The method is simple, rapid accurate and useful.
Hu
T
T
College of Stomatology, West China University of Medical Sciences.
Zhou
X
X
Zhang
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P
D010129:4-Aminobenzoic Acid / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003773:Dental Plaque / Q000382:microbiology; D006801:Humans; D013295:Streptococcus mutans / Q000378:metabolism; D013298:Streptococcus sanguis / Q000378:metabolism*
D010129:4-Aminobenzoic Acid