A Method of the Simultaneous Purification of Cardiac Troponin T and I.
A method of the simultaneous purification of cardiac troponin T (cTnT) and troponin I (cTnI) from human cardiac left ventricular muscle have been developed. Five mg cTnT and 10.2 mg of cTnI were obtained from 100 mg of cardiac muscle. The purity of cTnT and cTnI could reach to 97.6% and 97.2% respectively. Their immunoactivity and specificity have been identified by ELISA method.
Li
Zhi-Liang
ZL
Department of Cardiology, Zhujiang Hospital, The First Military Medical College, Guangzhou 510280, China.
Fu
Chao-Ping
CP
Qian
Xue-Xian
XX
Lu
Qing
Q
Wang
Su-Hua
SH
Li
Mei-Lan
ML