Expression of Chicken Smooth Muscle Myosin Light Chain Kinase in NIH 3T3 Cells.
The myosin light chain kinase (MLCK) is important in regulating the smooth muscle contraction. The pBKrsv-MLCK was obtained by inserting MLCK cDNA into pBKrsv polylinker and transfected into NIH 3T3 cells. The results revealed that the transfected cells can express chicken MLCK identified by DNA-PCR, RT-PCR and Western blot. The expressed MLCK has high activity, thus providing the basis for further investigation of the signal transduction and regulation of smooth muscle contraction of MLCK.
Wang
Yuan
Y
Department of Biochemistry and Molecular Biology Laboratory, Anhui Medical University, Hefei 230032, China.
Gui
Shu-Yu
SY
Watterson
D.M.
DM