title: Involvement of Jasmonic Acid in Elicitor-Induced Phytoalexin Production in Suspension-Cultured Rice Cells.
abstract: It has been suggested that jasmonic acid (JA) could be an integral part of a general signal transduction system regulating inducible defense genes in plants. It was reported that treatment with an elicitor (N-acetylchitoheptaose) induced production of phytoalexin in suspension-cultured rice (Oryza sativa L.) cells. In this study, the role of JA in the induction of phytoalexin production by N-acetylchitoheptaose was investigated. Exogenously applied ([plus or minus])-JA (10-4 M) clearly induced the production of momilactone A, a major phytoalexin, in suspension-cultured rice cells. On the other hand, in rice cells treated with N-acetylchitoheptaose, endogenous JA was rapidly and transiently accumulated prior to accumulation of momilactone A. Treatment with ibuprofen, an inhibitor of JA biosynthesis, reduced production of momilactone A in the cells treated with N-acetylchitoheptaose, but the addition of ([plus or minus])-JA increased production of momilactone A to levels higher than those in the elicited rice cells. These results strongly suggest that JA functions as a signal transducer in the induction of biosynthesis of momilactone A by N-acetylchitoheptaose in suspension-cultured rice cells.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan (H.N., M.S., H.Y., Y.N., T.O.).
authors.forename: H.
authors.initials: H
authors.lastname: Nojiri
authors.forename: M.
authors.initials: M
authors.lastname: Sugimori
authors.forename: H.
authors.initials: H
authors.lastname: Yamane
authors.forename: Y.
authors.initials: Y
authors.lastname: Nishimura
authors.forename: A.
authors.initials: A
authors.lastname: Yamada
authors.forename: N.
authors.initials: N
authors.lastname: Shibuya
authors.forename: O.
authors.initials: O
authors.lastname: Kodama
authors.forename: N.
authors.initials: N
authors.lastname: Murofushi
authors.forename: T.
authors.initials: T
authors.lastname: Omori