Strong antigenic selection shaping the immunoglobulin heavy chain repertoire of B-1a lymphocytes in lambda 2(315) transgenic mice.
The B lymphocyte compartment of the recombinant mouse line L2 consists predominantly of CD5(+) B-1 cells that exclusively express the transgenic (Tg) lambda 2 L chain of the plasmacytoma MOPC315. Using such Tg mice as a simplified model to study positive selection processes, we show that restriction to a single L chain results in a strongly oligoclonal IgH chain repertoire in fetal and neonatal liver-derived B cells, as well as in peritoneal CD5(+) B-1 lymphocytes from adult mice. In contrast, in vitro differentiated fetal liver Pro/PreB-I cells from L2 embryos show a clear increase of complementarity determining region three (CDR3) diversity. These data suggest that the antibody repertoire of B-1a cells is strongly selected by antigen during fetal as well as adult peritoneal phase.
Kretschmer
Karsten
K
Molecular Immunology, GBF, German Research Centre for Biotechnology, Braunschweig, Germany. kkr@gbf.de
Engel
Holger
H
Weiss
Siegfried
S
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000502:physiology; D022801:Complementarity Determining Regions / Q000737:chemistry; D005333:Fetus / Q000276:immunology; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D007072:Immunoglobulin D / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics / Q000502:physiology*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008099:Liver / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008969:Molecular Sequence Data
D000941:Antigens; D022801:Complementarity Determining Regions; D007072:Immunoglobulin D; D007143:Immunoglobulin Heavy Chains; D007133:Immunoglobulin Joining Region; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region