Lipoatrophic diabetes-associated utero-ovarian dysfunction: influence of cellular lipid deposition on norepinephrine indices.
OBJECTIVE
Elucidation of the intracellular lipoatrophic diabetic state and the concomitant alterations in norepinephrine (NE) parameters characterizing female reproductive failure.


METHODS
Quantitation of intrinsic NE levels in utero-ovarian and pancreatic tissue samples of C57BL/KsJ (+/?) control and (db/db) diabetic littermate mice was by high performance liquid chromatography (HPLC) and compared with the microspectrofluorometric histofluorescent (HF) localization of cellular and parenchymal NE.


RESULTS
Diabetes-associated elevations in HPLC-detectable tissue NE concentrations occurred in all pancreatic and reproductive tract tissue samples as compared to control-matched samples, whereas concurrent HF analysis revealed suppressed perivascular and parenchymal NE depositions in diabetic mice.


CONCLUSIONS
These data suggest that progressive hypertriglyceridemia/lipidemia may suppress the effectiveness of intrinsic elevations in tissue NE concentrations from effectively counterregulating the deleterious effects of the hyperglycemic, type-2 diabetic condition.
Garris
David R
DR
Division of Cell Biology and Biophysics, School of Biological Sciences and School of Medicine, University of Missouri-Kansas City, Mo. 64110-2499, USA. garrisd@umkc.edu
Garris
Bryan L
BL
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003593:Cytoplasm / Q000648:ultrastructure; D003923:Diabetes Mellitus, Lipoatrophic / Q000473:pathology / Q000503:physiopathology*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000473:pathology; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005260:Female; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D009638:Norepinephrine / Q000032:analysis*; D015388:Organelles / Q000648:ultrastructure; D010053:Ovary / Q000737:chemistry / Q000503:physiopathology* / Q000648:ultrastructure; D010179:Pancreas / Q000737:chemistry; D014280:Triglycerides / Q000097:blood; D014599:Uterus / Q000737:chemistry / Q000503:physiopathology* / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure; D015430:Weight Gain
D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D008055:Lipids; D014280:Triglycerides; D009638:Norepinephrine