Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants.
This paper demonstrates a simple but effective use of combinatorial probes to label plant chromosomes by multicolour fluorescence in situ hybridisation (FISH). Three different DNA probes were labelled with only two different fluorophores, hybridised to somatic metaphase chromosomes of Secale cereale and Triticum aestivum, simultaneously visualised, and unequivocally distinguished in a single FISH experiment. Combinatorial labelling can augment karyotypical investigations, physical mapping of chromosomes and other analyses in plants based upon FISH.
Hasterok
Robert
R
Department of Plant Anatomy and Cytology, University of Silesia, Katowice, Poland. hasterok@us.edu.pl
Langdon
Tim
T
Taylor
Steve
S
Jenkins
Glyn
G
D002875:Chromosomes / Q000502:physiology / Q000648:ultrastructure; D020650:Combinatorial Chemistry Techniques; D015342:DNA Probes; D018744:DNA, Plant / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008677:Metaphase; D010944:Plants / Q000235:genetics*; D012434:Secale / Q000235:genetics; D014908:Triticum / Q000235:genetics
D015342:DNA Probes; D018744:DNA, Plant