Antioxidative components of Psoralea corylifolia (Leguminosae).
A meroterpene and four flavonoids were isolated from the seeds of Psoralea corylifolia as antioxidative components. Their structures were elucidated by spectral data and identified as bakuchiol (1), bavachinin (2), bavachin (3), isobavachin (4) and isobavachalcone (5). In particular, meroterpene 1 and flavonoids 4 and 5 showed broad antioxidative activities in rat liver microsomes and mitochondria. They inhibited NADPH-, ascorbate-, t-BuOOH- and CCl(4)-induced lipid peroxidation in microsomes. They also prevented NADH-dependent and ascorbate-induced mitochondrial lipid peroxidation. Bakuchiol (1) was the most potent antioxidant in microsomes and the inhibition of oxygen consumption induced by lipid peroxidation was time-dependent. Furthermore, bakuchiol (1) protected human red blood cells against oxidative haemolysis. These phenolic compounds in P. corylifolia were shown to be effective in protecting biological membranes against various oxidative stresses.
Haraguchi
Hiroyuki
H
Faculty of Engineering, Fukuyama University, Gakuen-cho, Japan. haragu@bt.fubt.fuyukama-u.ac.jp
Inoue
Junji
J
Tamura
Yukiyoshi
Y
Mizutani
Kenji
K
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002599:Chalcone / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D047188:Chalcones; D004912:Erythrocytes / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D015394:Molecular Structure; D010101:Oxygen Consumption / Q000187:drug effects; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D029907:Psoralea; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D047309:Flavones
D000975:Antioxidants; D002599:Chalcone; D047188:Chalcones; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; C459212:bavachin; C468754:isobavachalcone; C468753:isobavachin; C012765:bakuchiol; C468752:bavachinin; D047309:Flavones