Sequence fidelity of a template-directed PNA-ligation reaction.
The ligation method and an appended duplex-stabilizing dye affect both yield and sequence selectivity of a template-controlled PNA-ligation. The highest selectivity was obtained with a peptide condensation that formed an abasic site.
Mattes
A
A
Max-Planck-Institut für Molekulare Physiologie, Otto-Hahn-Str. 11, D-44227 Dortmund, Germany.
Seitz
O
O
D000595:Amino Acid Sequence; D001483:Base Sequence; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D013698:Templates, Genetic
D009841:Oligonucleotides; D020135:Peptide Nucleic Acids