Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat.
Desmin content and immunohistochemical appearance were measured in tibialis anterior muscles of rats subjected to a single bout of 30 eccentric contractions (ECs). Ankle torque was measured before EC and at various recovery times, after which immunohistochemical and immunoblot analyses were performed. Torque decreased by approximately 50% immediately after EC and fully recovered 168 h later (P < 0.001). Loss of desmin staining was maximal 12 h after EC and recovered by 72 h. Immunoblots unexpectedly demonstrated a significant increase in the desmin-to-actin ratio by 72 h after EC (P < 0.01) and was still increasing after 168 h (P < 0.0001). These data demonstrate a relatively rapid qualitative loss of desmin immunostaining immediately after a single EC bout but a tremendous quantitative increase in desmin content 72-168 h later. This dynamic restructuring of the muscle's intermediate filament system may be involved in the mechanism of EC-induced muscle injury and may provide a structural explanation for the protective effects observed in muscle after a single EC bout.
Barash
Ilona A
IA
Department of Orthopaedics, and the Biomedical Sciences Graduate Group, University of California and Veterans Affairs Medical Centers, San Diego, California 92161, USA.
Peters
David
D
Fridén
Jan
J
Lutz
Gordon J
GJ
Lieber
Richard L
RL
D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism*; D003893:Desmin / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D003893:Desmin