[Human herpesvirus-8 detection in Kaposi's sarcoma, multiple myeloma, and lymphoproliferative syndromes occurring in immunocompetent and immunocompromised patients].
BACKGROUND
The purposes of this study were: to study the presence of human herpesvirus-8 (HHV-8) in different Kaposi's sarcoma (KS) epidemiological groups, multiple myeloma (MM), and immunodeficiency-associated lymphoid proliferations; to investigate the potential sexual transmission of HHV-8 by analyzing its presence in women from the general population, human immunodeficiency virus (HIV) seropositive women, and prostitutes; and to establish a reliable and efficient PCR strategy for the detection of HHV-8.


METHODS
HHV-8 detection was performed by PCR and positive cases were confirmed by automatic bi-directional sequencing. We selected 25 KS, 70 immunodeficiency associated non-Hodgkin's lymphomas (NHL), 30 HIV-positive Hodgkin's lymphomas (HL), and 2 primary effusion lymphomas (PEL). Bone marrow aspirates were available from 41 MM, 9 monoclonal gammopathies of undetermined significance and 24 patients with other disorders. Bone marrow dendritic cell cultures from 12 MM patients were also performed. Cells from cervical, anal, and oral cavity scrapes were examined for the presence of HHV-8 in 40 control women, 10 HIV-seropositive women, and 20 HIV-seronegative prostitutes. Serologic tests were also performed.


RESULTS
HHV-8 was specifically detected in 100% KS and PEL, and in 5.7% immunodeficiency associated NHL. All cases of HIV-HL and MM were HHV-8 negative. Antibodies against HHV-8 were found in 10% of control women, 10% HIV-positive women, and 25% prostitutes. Only 1 sample was positive for HHV-8 by PCR.


CONCLUSIONS
HHV-8 is associated with all epidemiological forms of KS; HHV-8 does not contribute to the pathogenesis of MM, and this virus is not ubiquitous in the human population. Seroprevalence of HHV-8 is increased in prostitutes, although this may partially be attributed to the geographical origin. For a reliable PCR detection of HHV-8, it is necessary to target different regions of the viral genome and to sequence amplification products.
Santón Roldán
Almudena
A
Departamento de Anatomía Patológica, Hospital Ramón y Cajal, Madrid, Spain.
De San José
Silvia
S
Gómez Sanz
Elvira
E
Fernández Muñoz
Rafael
R
Herrera
Pilar
P
Bellas Menéndez
Carmen
C
D001003:Anal Canal / Q000821:virology; D002584:Cervix Uteri / Q000821:virology; D005260:Female; D006566:Herpesviridae Infections / Q000453:epidemiology* / Q000276:immunology / Q000635:transmission; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D007121:Immunocompetence; D016867:Immunocompromised Host; D008223:Lymphoma / Q000276:immunology / Q000821:virology*; D008232:Lymphoproliferative Disorders / Q000276:immunology / Q000821:virology; D009061:Mouth Mucosa / Q000821:virology; D009101:Multiple Myeloma / Q000276:immunology / Q000821:virology*; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000276:immunology / Q000821:virology*; D016036:Seroepidemiologic Studies; D011477:Sex Work; D015229:Sexually Transmitted Diseases, Viral