Molecular cloning of HR1a and HR1b, high molecular hemorrhagic factors, from Trimeresurus flavoviridis venom.
HR1a and HR1b are two high molecular weight (P-III class) hemorrhagic factors in the venom of Trimeresurus flavoviridis. In this study, we cloned cDNAs of the HR1a and HR1b precursors and analyzed their nucleotide sequences. The cDNA for HR1a was 2368 nucleotides in length and encoded an open reading frame (ORF) of 609 amino acids; that for HR1b was 2237 nucleotides and encoded an ORF of 614 amino acids. Both cDNAs belonged to the N-III class consisting of signal, pro, metalloproteinase, disintegrin-like and cysteine-rich regions, and shared strong amino acid sequence similarity (74.4%). The HR1b precursor was found to have an additional seven amino acid sequence at the carboxyl terminus compared with a mature form of HR1b.
Kishimoto
Masaaki
M
Institute of Medicinal Chemistry, Hoshi University, 2-4-41 Ebara Shinagawa-ku, Tokyo 142-8501, Japan.
Takahashi
Tomoko
T
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D008666:Metalloendopeptidases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012689:Sequence Homology, Nucleic Acid; D017841:Trimeresurus; D014757:Viper Venoms / Q000737:chemistry / Q000235:genetics*
D003435:Crotalid Venoms; D018076:DNA, Complementary; C029748:Trimeresurus venoms; D014757:Viper Venoms; C065690:hemorrhagic toxin HR-1b; D008666:Metalloendopeptidases; C468284:hemorrhagic toxin HR-1a, Trimeresurus flavoviridis