title: Effects of chlorpromazine, pentoxifylline and dexamethasone on mRNA expression of lipopolysaccharide-induced inflammatory cytokines in bovine peripheral blood mononuclear cells.
abstract: The effects of chlorpromazine (CPZ), pentoxifylline (PTX) and dexamethasone (DEX) on mRNA expression of lipopolysaccharide (LPS)-induced proinflammatory cytokines were examined in bovine peripheral blood mononuclear cells (PBMCs) in vitro. The expression of inflammatory cytokine mRNAs was analyzed by RT-PCR and Southern blot hybridization in bovine PBMCs. CPZ and DEX decreased the expression of cytokine mRNA (such as interleukin-1 beta and tumor necrosis factor-alpha) after stimulation with LPS in a dose-dependent manner. However, pretreatment with PTX had no inhibitory effect on the mRNA expression of proinflammatory cytokines. These results indicated that pretreatment with CPZ and DEX might be effective to reduce the production of LPS-induced inflammatory cytokines in bovine PBMCs in vitro.
authors.affiliation: Department of Veterinary Pathology, large animal Clincal Center, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kiku
authors.forename: Hironori
authors.initials: H
authors.lastname: Matsuzawa
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Terasaki
authors.forename: Shigeo
authors.initials: S
authors.lastname: Fukuda
authors.forename: Satoru
authors.initials: S
authors.lastname: Kon-Nai
authors.forename: Masateru
authors.initials: M
authors.lastname: Koiwa
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Yokomizo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sato
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Rosol
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okada
authors.forename: Tomo-o
authors.initials: TO
authors.lastname: Yoshino
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D015139:Blotting, Southern; D002417:Cattle; D002746:Chlorpromazine / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D005786:Gene Expression Regulation; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D010431:Pentoxifylline / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D003907:Dexamethasone; D010431:Pentoxifylline; D002746:Chlorpromazine