title: Comparative profiles of intramacrophage behavior of Mycobacterium tuberculosis and Mycobacterium avium complex with different levels of virulence.
abstract: Mycobacterium tuberculosis (MTB) and M. avium complex (MAC) strains with different levels of virulence in mice were examined for profiles of interaction with murine peritoneal macrophages (Mphis). Their growth rates in Mphis were in these orders: H37Ra strain (attenuated) > H37Rv strain (virulent) for MTB, and N-260 strain (moderate virulence) > MAC N-444 strain (low virulence) for MAC. MTB but not MAC caused the necrotic death of host Mphis in terms of increased release of lactate dehydrogenase from infected Mphis. The MTB H37Ra strain induced a greater production of reactive nitrogen intermediates (RNI) by Mphis than the MTB H37Rv strain did. However, this phenomenon was not observed with MAC, implying less important roles of RNI in the expression of Mphi antimicrobial activity against MAC organisms.
authors.affiliation: Department of Microbiology and Immunology, Shimane Medical University, Izumo, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sano
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Sato
authors.forename: Chiaki
authors.initials: C
authors.lastname: Sano
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Shimizu
authors.forename: Haruaki
authors.initials: H
authors.lastname: Tomioka
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009162:Mycobacterium avium / Q000254:growth & development / Q000472:pathogenicity*; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000472:pathogenicity*; D026361:Reactive Nitrogen Species / Q000096:biosynthesis / Q000502:physiology; D014774:Virulence
chemical_list: D026361:Reactive Nitrogen Species; D007770:L-Lactate Dehydrogenase