Novel characterisation of the gene encoding conglutinin reveals that previously characterised promoter corresponds to the CL-43 promoter.
Conglutinin, a collectin found in bovine serum, is an opsonin that binds to glycoconjugates on the surface of microorganisms or on deposited iC3b, and acts in concert with phagocytes to establish a first-line of immune defense. We have isolated a genomic conglutinin phage clone and found that the 5'-flanking region shows 95.8% identity with the sequence previously published, which on the other hand shows 99.7% identity with the CL-43 promoter. We conclude that the previously published promoter corresponds to the CL-43 promoter and that the functional studies performed on it apply to transcription of CL-43. Comparison of potential cis-regulatory elements in relation to the functional studies indicates that the two genes are regulated by different mechanisms.
Hansen
Soren
S
Department of Immunology and Microbiology, University of Southern Denmark, Odense, Winsloewparken 21.1, DK-5000 Odense C, Denmark.
Moeller
Vivi
V
Holm
Dorte
D
Vitved
Lars
L
Bendixen
Christian
C
Skjodt
Karsten
K
Holmskov
Uffe
U
D001483:Base Sequence; D037222:Collectins / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012712:Serum Globulins / Q000235:genetics*
D037222:Collectins; D012712:Serum Globulins; C024014:conglutinin; C086954:collectin-43 protein, bovine