title: Regeneration of natural antibody repertoire after massive ablation of lymphoid system: robust selection mechanisms preserve antigen binding specificities.
abstract: Natural Abs (NAbs) are Igs present in the serum and body fluids of healthy vertebrate animals, without any previous intentional immunization. NAbs often exhibit autoreactivity but also play an essential role in immunity, being a first line of defense against infectious microorganisms. We have previously analyzed the natural serum IgM Ab repertoire of normal mice, characterizing their reactivity with hundreds/thousands of self Ags; a significant similarity among different individuals was observed, and it was found that many reactivities of NAbs stably kept during adulthood were established early in life, implicating that period as a critical time window in the physiology of NAb repertoire selection. In the work reported here, experiments were conducted to address the role of normal lymphocyte ontogeny to the formation and stability of adult NAb repertoire. The massive destruction of the lymphoid system was promoted in adult mice with gamma-irradiation, and regeneration of hemopoietic tissues was granted by bone marrow or fetal liver inoculum. NAb repertoire regeneration was followed for 60 days after gamma-irradiation in bone marrow or fetal liver chimeric animals. The analysis of serum IgM reactivity with hundreds/thousands of self Ags showed that the NAb repertoire regenerated most of its original format after massive destruction of lymphoid compartments, characterizing autoreactive repertoire selection as a robust biological process. The data also show that regeneration of the NAb repertoire occurred similarly in fetal liver and bone marrow chimeras, although the latter animals poorly reconstituted their CD5(+) B1 cell compartment, suggesting that B1 cells are not essential for natural Ab regeneration.
authors.affiliation: Institut Pasteur, Paris, France. Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Brazil. alberton@acd.ufrj.br
authors.forename: Alberto
authors.initials: A
authors.lastname: Nobrega
authors.forename: Beatriz
authors.initials: B
authors.lastname: Stransky
authors.forename: Nathalie
authors.initials: N
authors.lastname: Nicolas
authors.forename: Antonio
authors.initials: A
authors.lastname: Coutinho
mesh_terms: D000375:Aging / Q000235:genetics / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity / Q000235:genetics; D001324:Autoantigens / Q000276:immunology / Q000378:metabolism*; D001666:Binding Sites, Antibody / Q000235:genetics; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D016332:Fetal Tissue Transplantation / Q000276:immunology; D007113:Immunity, Innate / Q000235:genetics; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000097:blood; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000276:immunology / Q000378:metabolism; D008212:Lymphocyte Depletion / Q000379:methods; D008221:Lymphoid Tissue / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000276:immunology / Q000378:metabolism; D011828:Radiation Chimera / Q000276:immunology
chemical_list: D001324:Autoantigens; D007075:Immunoglobulin M; D007136:Immunoglobulins