Maltooligosaccharide disproportionation reaction: an intrinsic property of amylosucrase from Neisseria polysaccharea.
Amylosucrase from Neisseria polysaccharea (AS) is a remarkable transglycosidase of family 13 of the glycoside hydrolases that catalyses the synthesis of an amylose-like polymer from sucrose and is always described as a sucrose-specific enzyme. Here, we demonstrate for the first time the ability of pure AS to catalyse the disproportionation of maltooligosaccharides by cleaving the alpha-1,4 linkage at the non-reducing end of a maltooligosaccharide donor and transferring the glucosyl unit to the non-reducing end of another maltooligosaccharide acceptor. Surprisingly, maltose, maltotriose and maltotetraose are very poor glucosyl donors whereas longer maltooligosaccharides are even more efficient glucosyl donors than sucrose. At least five glucose units are required for efficient transglucosylation, suggesting the existence of strong binding subsites, far from the sucrose binding site, at position +4 and above.
Albenne
Cécile
C
Centre de Bioingénierie Gilbert Durand, UMR CNRS 5504, UMR INRA 792, INSA, 135 avenue de Rangueil, 31077 Cedex 4, Toulouse, France.
Skov
Lars K
LK
Mirza
Osman
O
Gajhede
Michael
M
Potocki-Véronèse
Gabrielle
G
Monsan
Pierre
P
Remaud-Simeon
Magali
M
D002236:Carbohydrate Conformation; D005964:Glucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D007700:Kinetics; D008320:Maltose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D009343:Neisseria / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D013395:Sucrose / Q000737:chemistry; D014312:Trisaccharides / Q000737:chemistry / Q000378:metabolism
D009844:Oligosaccharides; D014312:Trisaccharides; C021705:maltooligosaccharides; D013395:Sucrose; C008317:maltotriose; D008320:Maltose; C009819:maltotetraose; D005964:Glucosyltransferases; C019700:amylosucrase