title: Influence of Elevated Fructose-2,6-Bisphosphate Levels on Starch Mobilization in Transgenic Tobacco Leaves in the Dark.
abstract: The aim of this work was to study the effect of elevated fructose-2,6-bisphosphate (Fru-2,6-bisP) levels on carbohydrate metabolism in leaves in the dark. In transgenic tobacco (Nicotiana tabacum L.) lines containing mammalian 6-phosphofructo-2-kinase activity there is an inverse relationship between the level of Fru-2,6-bisP in leaves and the rate of starch breakdown in the dark. Estimates of the flux response coefficient for the rate of net starch degradation with respect to changes in Fru-2,6-bisP level are -0.57 for whole leaves and -0.69 to -0.89 for excised leaf discs. We suggest that this decrease in the net rate of starch breakdown is caused, at least in part, by stimulation of unidirectional starch synthesis. Measurements of the levels of metabolic intermediates and the metabolism of [U-14C]glucose indicate that the stimulation of starch synthesis in the dark is a result of high Fru-2,6-bisP levels, increasing the 3-phosphoglycerate:inorganic phosphate ratio in leaves. We argue that the observed response to changes in the level of Fru-2,6-bisP are effected through activation of pyrophosphate:fructose-6-phosphate 1-phosphotransferase. However, the extent to which changes in Fru-2,6-bisP influence starch metabolism in wild-type plants is not known.
authors.affiliation: Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom.
authors.forename: P.
authors.initials: P
authors.lastname: Scott
authors.forename: N. J.
authors.initials: NJ
authors.lastname: Kruger