title: The respiratory burst activity of bottlenose dolphin neutrophils elicited by several stimulants.
abstract: To investigate the early host defense function in aquatic animals, the respiratory burst activity of bottlenose dolphin neutrophils against soluble and particulate stimulants was measured by luminol-dependent chemiluminescence assays and compared with those of bovine and human. Dolphin neutrophils generated the respiratory burst in response to phorbol 12-myristate 13-acetate (PMA), concanavalinA (ConA), heated-plasma (HP), and homologous-plasma opsonized zymosan except N-formyl-Met-Leu-Phe (fMLP). However, the respiratory burst of dolphin neutrophils stimulated by lipopolysaccharide and Staphylococcus aureus was inferior to those of bovine and human. Furthermore, DP-OZ also induced the respiratory burst of bovine and human neutrophils. In conclusion, dolphin neutrophils responded to several soluble and particulate stimulants as well as human neutrophils, but were refractory or slightly responded to bacterial agents.
authors.affiliation: Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan.
authors.forename: Rikiya
authors.initials: R
authors.lastname: Shiraishi
authors.forename: Takuya
authors.initials: T
authors.lastname: Itou
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sugisawa
authors.forename: Youko
authors.initials: Y
authors.lastname: Shoji
authors.forename: Tomoko
authors.initials: T
authors.lastname: Endo
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000818:Animals; D002417:Cattle; D004289:Dolphins / Q000276:immunology*; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010940:Plant Proteins / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects*; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D015054:Zymosan / Q000494:pharmacology*
chemical_list: D008070:Lipopolysaccharides; D010940:Plant Proteins; C006882:canavalin protein, Canavalia ensiformis; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate