FRET probes to monitor phospholipase A2 activity.
Phosphatidylethanolamine and -choline derivatives equipped with fluorescent donor-acceptor pairs of dyes connected to the tips of the fatty acids were synthesised and shown to be suitable substrates for phospholipase A2.
Wichmann
O
O
Max-Planck-Institut für molekulare Physiologie, Otto-Hahn-Str. 11, 44227 Dortmund, Germany.
Schultz
C
C
D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000138:chemical synthesis / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000138:chemical synthesis / Q000737:chemistry*; D010741:Phospholipases A / Q000737:chemistry* / Q000378:metabolism*; D054467:Phospholipases A2; D013379:Substrate Specificity
D005456:Fluorescent Dyes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010741:Phospholipases A; D054467:Phospholipases A2