title: Ribonucleotide reductase subunits from the yellow fever mosquito, Aedes aegypti: cloning and expression.
abstract: Ribonucleotide reductase catalyses the de novo synthesis of deoxyribonucleotides. Class I reductases use an iron center to generate a tyrosyl free radical that can initiate formation of the deoxyribonucleotide. These reductases are alpha 2 beta 2 holoenzymes, and the subunits are denoted as R1 and R2. R1 contains the allosteric binding site and the active site, whereas R2 contains a binuclear iron center that initiates formation of the tyrosyl radical. We have cloned and sequenced the cDNAs encoding the R1 and R2 subunit in the yellow fever mosquito, Aedes aegypti. The messages for these proteins are increased in response to blood-feeding.
authors.affiliation: Department of Biological Sciences, University of Wisconsin-Parkside, Kenosha, WI 53141-2000, USA. daphne.pham@uwp.edu
authors.forename: D Q D
authors.initials: DQ
authors.lastname: Pham
authors.forename: B J
authors.initials: BJ
authors.lastname: Blachuta
authors.forename: H
authors.initials: H
authors.lastname: Nichol
authors.forename: J J
authors.initials: JJ
authors.lastname: Winzerling
mesh_terms: D000330:Aedes / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D012264:Ribonucleotide Reductases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D012264:Ribonucleotide Reductases; C108427:ribonucleotide reductase R2 subunit