Separation and characterization of sub-microm- and microm-sized particles by capillary zone electrophoresis.
The analytical separation and characterization of particles in the size range of sub-microm and microm diameters by capillary zone electrophoresis (CZE) has been reviewed. The theoretical basis, on which the mobility can be interpreted to provide information regarding characteristics of particle surface, has shortly been presented. Particular emphasis was put on the model dependence of that interpretation and the need in most applications to forego the classical idealized model of spherical particles with "smooth" surfaces and to apply more realistic models, which take the "hairy" surface of real particles into account. Some highlights of the literature on the CZE of polystyrene latex microspheres, organic and inorganic colloids, lipoprotein particles, viruses, liposomes, biological membrane vesicles, and biological cells have been discussed. Also summarized are the reports on the particle size dependence of mobility and peak broadening in CZE and on electrophoretic behavior of rodlike particles and particle aggregates. Finally, the effects of neutral polymers in the background electrolyte on particle mobility and peak width are reviewed.
Radko
Sergey P
SP
Biotechnology Department, Research Center for Medical Genetics, Russian Academy of Medical Sciences, Moscow, Russia.
Chrambach
Andreas
A
D003102:Colloids / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D007202:Indicators and Reagents; D008081:Liposomes / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D003102:Colloids; D007202:Indicators and Reagents; D008081:Liposomes