Behavior of HO-1-N-1, buccal mucosa carcinoma derived cells, on [Ca2+]i responses to stimulants.
Buccal mucosa carcinoma-derived cell line, HO-1-N-1, epithelial-like cells, was obtained in order to investigate the characteristics of oral cancer cells and examine the [Ca2+]i responses to stimulants, such as bradykinin (BK), histamine (HIST), thapsigargin (TG), epidermal growth factor (EGF) and transforming growth factor alpha (TGF alpha ). Intracellular Ca2+ influx was observed by all stimulants that enhanced the [Ca2+]i response. However, intracellular Ca2+ release was not observed in response to growth factors. The [Ca2+]i response of BK (100 nM) was inhibited by 10 micro M of the BKB2 antagonist, D-Arg-[Hyp3, Thi5,8, D-Phe7]-BK, and HIST (1 mM) was completely inhibited by 100 nM of the H1 antagonist, (+)-chlorpheniramine, in the presence and absence of extracellular Ca2+ (1.5 mM).
Arai
Takafumi
T
Department of Pharmacology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Matsumoto
Hiroko
H
Akimoto
Yoshiaki
Y
Nishimura
Hitoshi
H
Ono
Makiko
M
Fujii
Akira
A
D001920:Bradykinin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects*; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006801:Humans; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D011956:Receptors, Cell Surface / Q000378:metabolism; D013268:Stimulation, Chemical; D019284:Thapsigargin / Q000494:pharmacology; D016211:Transforming Growth Factor alpha / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*
D006634:Histamine H1 Antagonists; D011956:Receptors, Cell Surface; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor; D019284:Thapsigargin; D006632:Histamine; D001920:Bradykinin; D002118:Calcium