title: The prognostic value of p27 in gastric cancer.
abstract: OBJECTIVE
  p27 is a cyclin-dependent kinase inhibitor and a putative tumor suppressor preventing progression of the cell cycle from G1 phase. Recent studies have suggested loss of p27 to correlate with poor prognosis in patients with a variety of solid tumors. Results in gastric cancer are contradictory. We therefore decided to study the potential of p27 as a prognostic marker in a consecutively surgically treated, single-institution series of patients.
  
  
  METHODS
  Using a monoclonal antibody against p27, immunohistochemistry was performed in paraffin-embedded tumor specimens from 316 patients.
  
  
  RESULTS
  Loss of p27 immunoreactivity (< or = 5% of the cancer cell nuclei positive) was observed in 241 (76%) out of 316 stained tumors. We observed no significant correlation between the expression of p27 and stage of disease, tumor size, depth of tumor invasion, lymph node metastases, distant metastases, Laurén classification, Borrmann type, grade of differentiation, age or gender. There was no significant difference in gastric cancer specific overall survival between patients with low and high p27 expression.
  
  
  CONCLUSIONS
  Our results add further doubt to the usefulness of p27 as a prognostic marker in gastric cancer.
authors.affiliation: Department of Surgery, University of Helsinki, Helsinki, Finland.
authors.forename: Jan-Patrik
authors.initials: JP
authors.lastname: Wiksten
authors.forename: Johan
authors.initials: J
authors.lastname: Lundin
authors.forename: Stig
authors.initials: S
authors.lastname: Nordling
authors.forename: Arto
authors.initials: A
authors.lastname: Kokkola
authors.forename: Kristina
authors.initials: K
authors.lastname: von Boguslawski
authors.forename: Caj
authors.initials: C
authors.lastname: Haglund
mesh_terms: D000368:Aged; D018797:Cell Cycle Proteins / Q000032:analysis* / Q000235:genetics; D050760:Cyclin-Dependent Kinase Inhibitor p27; D005260:Female; D005500:Follow-Up Studies; D005753:Gastric Mucosa / Q000473:pathology; D016147:Genes, Tumor Suppressor; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology* / Q000601:surgery; D016019:Survival Analysis; D013997:Time Factors; D025521:Tumor Suppressor Proteins / Q000032:analysis* / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27