title: Detection of equine Babesia spp. gene fragments in Dermacentor nuttalli Olenev 1929 infesting mongolian horses, and their amplification in egg and larval progenies.
abstract: Babesia equi (EMA-1) and Babesia caballi (BC48) gene fragments were amplified by polymerase chain reaction (PCR), in blood samples, and partially fed-females and egg and larval progenies of Dermacentor nuttalli, collected from horses in Altanbulag, Tuv Province, Mongolia. While Babesia parasite DNA was detected in some horse blood samples during the first PCR, all positive cases in partially fed-female ticks, eggs and larvae were confirmed by nested PCR. Present study reinforces earlier similar findings in unfed D. nuttalli ticks collected from an open space vegetation in Bayanonjuul, Tuv Province in Central Mongolia, pointing to the most likely important role of D. nuttalli in the transmission of equine babesiosis in Mongolia. The detection of parasite DNA in eggs and larval progenies is likewise suggestive of transovarial parasite transmission in this tick species.
authors.affiliation: Department of Basic Veterinary Science, the United Graduate School of Veterinary Sciences, Gifu University, Yanagito, Japan.
authors.forename: Badgar
authors.initials: B
authors.lastname: Battsetseg
authors.forename: Susana
authors.initials: S
authors.lastname: Lucero
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
authors.forename: Florencia
authors.initials: F
authors.lastname: Claveria
authors.forename: Badarch
authors.initials: B
authors.lastname: Byambaa
authors.forename: Banzragch
authors.initials: B
authors.lastname: Battur
authors.forename: Damdinsuren
authors.initials: D
authors.lastname: Boldbaatar
authors.forename: Zayat
authors.initials: Z
authors.lastname: Batsukh
authors.forename: Tsevegmed
authors.initials: T
authors.lastname: Khaliunaa
authors.forename: Gonchigoo
authors.initials: G
authors.lastname: Battsetseg
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Nagasawa
authors.forename: Kozo
authors.initials: K
authors.lastname: Fujisaki
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000469:parasitology*; D001403:Babesia / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D001404:Babesiosis / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D003870:Dermacentor / Q000469:parasitology*; D018562:Disease Transmission, Infectious / Q000662:veterinary; D005260:Female; D005784:Gene Amplification; D006734:Horse Diseases / Q000469:parasitology* / Q000635:transmission; D006736:Horses; D008297:Male; D008986:Mongolia; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D016054:DNA, Protozoan