title: Gelatinase B deficiency protects against endotoxin shock.
abstract: Gelatinase B or matrix metalloproteinase-9 (MMP-9) is stored in the tertiary granules of polymorphonuclear leukocytes. These cells are key effectors in acute inflammatory diseases such as sepsis. Endotoxin leads to rapid release of gelatinase B from these granules in vitro and in vivo, but the role of this enzyme in bacterial sepsis and endotoxin shock remains unclear. We studied the clinical course of endotoxinemia and its relation with the expression of gelatinase B from the pool of circulating leukocytes in adult as well as in young mice in a model of endotoxin-induced shock and compared wild-type with gelatinase B-deficient mice. The gelatinase B-deficient mice were resistant to endotoxin shock, which implies that specific MMP-9 inhibition constitutes anapproach for the treatment of septic shock syndromes.
authors.affiliation: Rega Institute, Laboratory of Molecular Immunology, University of Leuven, Leuven, Belgium.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Dubois
authors.forename: Sofie
authors.initials: S
authors.lastname: Starckx
authors.forename: Axel
authors.initials: A
authors.lastname: Pagenstecher
authors.forename: Joost van den
authors.initials: Jv
authors.lastname: Oord
authors.forename: Bernd
authors.initials: B
authors.lastname: Arnold
authors.forename: Ghislain
authors.initials: G
authors.lastname: Opdenakker
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D007962:Leukocytes / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology; D020780:Matrix Metalloproteinase 9 / Q000172:deficiency* / Q000378:metabolism / Q000502:physiology; D020782:Matrix Metalloproteinases / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D012772:Shock, Septic / Q000517:prevention & control*; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics
chemical_list: D008070:Lipopolysaccharides; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases; D020780:Matrix Metalloproteinase 9