title: Protective effect of polypeptide from Chlamys farreri on hairless mice damaged by ultraviolet A.
abstract: OBJECTIVE
  To study the protective effect of the polypeptide isolated from Chlamys farreri (PCF) on hairless mice skin damaged by ultraviolet A.
  
  
  METHODS
  Enzymes and malondialdehyde (MDA) were determined by biochemical methods; the expressions of Bcl-2 protein and NOS protein were examined by immunohistochemical technique. The ultra-structure of the skin was observed through electronic microscope.
  
  
  RESULTS
  PCF could enhance the activities of glutathione peroxidase (GSH-px), superoxide dismutase (SOD), and total anti-oxidative capacity (T-AOC). Also PCF could reduce the amount of MDA, increase the expression of Bcl-2 protein, and inhibit the expression of NOS protein. The ultra-structure of epidermis and fibroblasts remained normal in 20 % PCF groups; there were vacuoles in smooth endoplasm reticulum in epidermis of mice and the number of rough endoplasm reticulum in fibroblasts was decreased in model group.
  
  
  CONCLUSIONS
  PCF had the protective effects on hairless mice skin damaged by ultraviolet A via its anti-oxidative mechanisms.
authors.affiliation: Medical College Qingdao University, Qingdao 266021, China.
authors.forename: Chun-Bo
authors.initials: CB
authors.lastname: Wang
authors.forename: Ru-Yong
authors.initials: RY
authors.lastname: Yao
authors.forename: Zhan-Tao
authors.initials: ZT
authors.lastname: Liu
authors.forename: Wei-Zhen
authors.initials: WZ
authors.lastname: Zhong
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Liu
authors.forename: Yue-Jun
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D051379:Mice; D008812:Mice, Hairless; D008974:Mollusca / Q000737:chemistry*; D010455:Peptides / Q000627:therapeutic use*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D012867:Skin / Q000528:radiation effects / Q000648:ultrastructure; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D010455:Peptides; D019253:Proto-Oncogene Proteins c-bcl-2; D011837:Radiation-Protective Agents