Differential post-transcriptional regulations of wnt mRNAs upon axolotl meiotic maturation.
The products of the Wntgene family play an essential role in several aspects of embryo patterning. We have investigated the post-transcriptional regulation of three of these genes: Awnt-1, Awnt-5A and Awnt-5B during axolotl (Ambystoma mexicanum) oogenesis, oocyte maturation and early development. We show that Awnt-1, Awnt-5A and Awnt-5B mRNAs are maternally expressed. The three transcripts are tightly regulated at specific times and display differential mRNA stability, poly(A) tail length and localization. In contrastto Awnt-5Bwhich is restricted to the animal hemisphere, Awnt-1 and Awnt-5A have no particular localization in stage VI oocytes. Interestingly, these two mRNAs exhibit a polyadenylation gradient along the animal-vegetal axis. Moreover, after meiotic maturation, Awnt-1 and 5A mRNAs become exclusively localized to the animal pole. This isthe first evidence of a complete mRNA re-localization to the animal hemisphere during oocyte maturation.
Vaur
Sabine
S
Laboratoire des régulationspost-transcriptionnelles, Institut André Lwoff, CNRS, UPR 1983, Villejuif, France.
Montreau
Nicole
N
Dautry
François
F
Andéol
Yannick
Y
D000557:Ambystoma; D000818:Animals; D015152:Blotting, Northern; D005306:Fertilization; D017403:In Situ Hybridization; D008540:Meiosis; D009865:Oocytes / Q000378:metabolism; D011061:Poly A; D026723:Polyadenylation; D011506:Proteins / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; D029961:Zebrafish Proteins
D011506:Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D051153:Wnt Proteins; C080089:Wnt-5A protein, Ambystoma mexicanum; C080090:Wnt-5B protein, Ambystoma mexicanum; D000071818:Wnt-5a Protein; D029961:Zebrafish Proteins; D011061:Poly A