title: A-type natriuretic peptide receptor in the spontaneously hypertensive rat kidney.
abstract: Renal NPR-A binding characteristics was examined in SHR. Renal ANP binding sites of NPR-A showed a lower maximal binding capacity and higher affinity in SHR than in WKY at all intrarenal sites. Despite the lower B(max) in SHR, both ANP(1-28) and ANP(5-25) stimulate similar or greater cGMP production in isolated glomeruli. Studies on guanylate cyclase from glomerular and papillary membranes have reported an increased basal and stimulated guanylate cyclase activity in SHR. The present study provides further evidences for altered NPR-A receptors in SHR kidney, which might act as a negative feedback in response to hypertension.
authors.affiliation: Physiological Laboratory, University of Cambridge, Downing Street, Cambridge, UK. geoffreyw@intra.niddk.nih.gov
authors.forename: Geoffrey E
authors.initials: GE
authors.lastname: Woodard
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Juan A
authors.initials: JA
authors.lastname: Rosado
authors.forename: John
authors.initials: J
authors.lastname: Brown
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006162:Guanylate Cyclase / Q000096:biosynthesis* / Q000378:metabolism; D006973:Hypertension / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000378:metabolism; D008024:Ligands; D008297:Male; D011485:Protein Binding; D011869:Radioligand Assay; D051381:Rats; D011918:Rats, Inbred SHR; D017461:Receptors, Atrial Natriuretic Factor / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D008024:Ligands; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; C091715:atrial natriuretic factor receptor A; D006152:Cyclic GMP