Galactosylated N-vinylpyrrolidone-maleic acid copolymers: synthesis, characterization, and interaction with lectins.
Water-soluble artificial glycoconjugate polymers were synthesized from poly(N-vinylpyrrolidone-co-maleic anhydride) by amidation with an amine-containing galactose derivative. The glycopolymers having different galactose contents were fully characterized in terms of chemical structure by NMR and potentiometric titrations, and their aqueous behavior was studied by viscometric measurements. Their specific binding properties were examined by enzyme-linked lectin assays using RCA(120) lectin. Whatever the glycopolymer, the grafted galactoses were shown to behave similarly to free galactose.
Auzély-Velty
Rachel
R
Centre de Recherches sur les Macromolécules Végétales, BP53, 38041 Grenoble cedex 9, France. rachel.auzely@cermav.cnrs.fr
Cristea
Mariana
M
Rinaudo
Marguerite
M
D016503:Drug Delivery Systems; D005690:Galactose / Q000737:chemistry*; D006031:Glycosylation; D037102:Lectins / Q000378:metabolism*; D008298:Maleates / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D011205:Povidone / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D012995:Solubility; D014783:Viscosity
D037102:Lectins; D008298:Maleates; C114335:maleic acid-vinylpyrrolidone copolymer; D011205:Povidone; D005690:Galactose