Effect of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in mice.
The distinctive properties of Brucella outer membrane have been considered to be critical for Brucella sp. virulence. Among the outer membrane molecules possibly related to these properties, Omp10 and Omp19 are immunoreactive outer membrane lipoproteins. Moreover, these proteins of Brucella could constitute a new family of outer membrane proteins specifically encountered in the family RHIZOBIACEAE: We evaluated the impact of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in mice. The omp10 mutant was dramatically attenuated for survival in mice and was defective for growth in minimal medium but was not impaired in intracellular growth in vitro, nor does it display clear modification of the outer membrane properties. Significantly fewer brucellae were recovered from the spleens of mice infected with the omp19 mutant than from those of mice infected with the parent strain at 4 and 8 weeks postinfection. The omp19 mutant exhibited an increase in sensitivity to the polycation polymyxin B and to sodium deoxycholate. These results indicate that inactivation of the omp19 gene alters the outer membrane properties of B. abortus.
Tibor
Anne
A
Unité de Recherche en Biologie Moléculaire, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium. anne.tobor@fundp.ac.be
Wansard
Valérie
V
Bielartz
Valery
V
Delrue
Rose-May
RM
Danese
Isabelle
I
Michel
Patrick
P
Walravens
Karl
K
Godfroid
Jacques
J
Letesson
Jean-Jacques
JJ
D000818:Animals; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000502:physiology; D002003:Brucella abortus / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology; D002006:Brucellosis / Q000209:etiology / Q000382:microbiology; D002417:Cattle; D002460:Cell Line; D005260:Female; D017353:Gene Deletion; D005798:Genes, Bacterial; D006367:HeLa Cells; D006801:Humans; D008074:Lipoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins; C097318:OMP10 protein, Brucella melitensis biovar Abortus; C097319:OMP19 protein, Brucella abortus