Cimetidine prevents suppression of delayed hypersensitivity in an animal model of haemorrhagic shock.
BACKGROUND
Cimetidine reverses immunosuppression following trauma, however, its effect on pure haemorrhagic shock is unknown.


METHODS
Mice sensitized by injection of sheep red blood cells (SRBCs), were subjected to cardiac puncture and randomized to a control group-A (n=11) and three shock groups (35% of blood volume extracted): group-S had no treatment (n=16), group-CP received cimetidine 50mg/kg intraperitoneally (n=16), group-CW received oral cimetidine (200mg/kg per day, n=16). After 5 days, animals were challenged by injection of SRBCs into the foot-pad of the right hind paw (same volume of saline was injected into left paw). Foot-pad thickness ratios (FPTRs) were determined at 16 and 40 h, and inflammatory response was assessed histologically.


RESULTS
At 16 h, FPTRs were greater in group-CW than group-S (P=0.01). There were no differences at 40 h. More animals in groups-CP and -CW had grade 3/4 inflammation, whilst group-S had the least inflammatory response (NS).


CONCLUSIONS
Cimetidine prevents suppression of delayed hypersensitivity in this model.
Hewitt
Peter MacD
PM
Department of Surgery, Cancer Research Laboratories, University of New South Wales and St. George Hospital, NSW 2217, Sydney, Australia.
Armstrong
Neil
N
Bowrey
Patricia
P
Cherian
Maya
M
Morris
David Lawson
DL
D000284:Administration, Oral; D000818:Animals; D016009:Chi-Square Distribution; D002927:Cimetidine / Q000008:administration & dosage* / Q000627:therapeutic use; D017707:Erythrocyte Transfusion; D006635:Histamine H2 Antagonists / Q000008:administration & dosage* / Q000627:therapeutic use; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D012756:Sheep; D012771:Shock, Hemorrhagic / Q000276:immunology*
D006635:Histamine H2 Antagonists; D002927:Cimetidine