Cellulose amphiphilic adsorbent for the removal of low density lipoprotein.
Cellulose adsorbent with amphiphilic ligands for adsorption of low density lipoprotein (LDL) was prepared by the following procedures: Cellulose beads were reacted with cholesterol N-(6-isocyanatohexyl) carbamate in the presence of pyridine in DMSO at 80 degrees C in order to introduce the hydrophilic moiety, it was then reacted with chlorosulfonic acid in dimethyl formamide, which introduced the sulfonic group. Adsorption capacity of adsorbent was studied, which showed the removal of LDL, TC, TG to be 0.857 mg/ml, 1.317 mg/ml, 1.002 mg/ml respectively without significantly affecting total protein levels in the plasma. Moreover, it has a better selectivity in removing LDL, TC, TG compared with cellulose adsorbent with only hydrophobic or hydrophilic ligand.
Shenqi
Wang
W
Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, China. sanyiwang@yahoo.com
Yaoting
Yu
Y
Tao
Cui
C
Yan
Cheng
C
D000327:Adsorption; D000368:Aged; D002482:Cellulose / Q000138:chemical synthesis* / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood; D007700:Kinetics; D008024:Ligands; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification*; D008863:Microspheres; D008875:Middle Aged; D013431:Sulfates / Q000737:chemistry; D013501:Surface-Active Agents / Q000138:chemical synthesis* / Q000737:chemistry
D008024:Ligands; D008077:Lipoproteins, LDL; D013431:Sulfates; D013501:Surface-Active Agents; D002482:Cellulose; D002784:Cholesterol