title: [Chronobiologic contrast of expression of p53, p16, and cyclin D1 in hepatocarcinoma cells].
abstract: OBJECTIVE
  To contrast the feature of different periods of p53, p16, and cyclin D1 expression in cells of hepatocarcinoma.
  
  
  METHODS
  Sixty hepatocarcinoma specimens were collected and divided into three periods in one yearly cycle with each period of 20 cases. Hepatocellular carcinoma was documented in all cases by pathomorphological classification. Edmondson-Steinet grading belonged to II~III stages. The expression of gene proteins of p53, p16, and cyclin D1 was determined by immunohistochemical assay (S-P method). The expressive intensity was analysed by the rank test.
  
  
  RESULTS
  p16 expression showed significant difference of period (H=10.334, P<0. 05). Arpil-July was the high expressive period.
  
  
  CONCLUSIONS
  p16 may take an important role in the chronobiologic mechanism of gene control of hepatocyte canceration and hepatocarcinoma cell growth.
authors.affiliation: Department of CT, Second Affiliated Hospital, Jiangxi Medical University. Nanchang 330006, China.
authors.forename: Wan Xian
authors.initials: WX
authors.lastname: Yu
authors.forename: Yong Hong
authors.initials: YH
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Cai
authors.forename: Xiao Jun
authors.initials: XJ
authors.lastname: Zhou
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000737:chemistry*; D019938:Cyclin D1 / Q000032:analysis*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1