title: Expression of the filarial nematode phosphorylcholine-containing glycoprotein, ES62, is stage specific.
abstract: ES62, an immunomodulatory phosphorylcholine-containing glycoprotein secreted by the rodent filarial nematode Acanthocheilonema viteae, has previously been shown to be produced by L4 larvae and adult worms only. However, homologous sequences to ES62 have recently been found in L1 and L3 cDNA libraries of certain human filarial nematodes. Therefore, the various stages of A. viteae were re-examined and it was again found that only the post-L3 stages secreted ES62. Synthesis but not secretion by earlier stages was ruled out by examination of the protein content of whole worm extracts and by immunoelectron microscopy. However, examination by PCR of the mRNA for ES62 revealed that it was found in the L1 and L3 larvae. This may explain why homologous sequences to ES62 have been found in Brugia malayi and Onchocerca volvulus larval cDNA libraries. It also suggests that filarial nematodes, in general, may secrete ES62. To obtain evidence for this, we investigated production by Brugia pahangi, a close relation of B. malayi. We found that ES62 was indeed secreted but, as with A. viteae, only by the post-L3 stages, although again the mRNA for ES62 could be detected in the earlier stages. Overall our results suggest that production of ES62 is not species specific, that it is indeed stage specific, and that this may be due to post-transcriptional control of expression.
authors.affiliation: Department of Immunology, University of Strathclyde, Glasgow.
authors.forename: G
authors.initials: G
authors.lastname: Stepek
authors.forename: M
authors.initials: M
authors.lastname: Auchie
authors.forename: R
authors.initials: R
authors.lastname: Tate
authors.forename: K
authors.initials: K
authors.lastname: Watson
authors.forename: D G
authors.initials: DG
authors.lastname: Russell
authors.forename: E
authors.initials: E
authors.lastname: Devaney
authors.forename: W
authors.initials: W
authors.lastname: Harnett
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D017179:Brugia pahangi / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D004153:Dipetalonema / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D018507:Gene Expression Regulation, Developmental; D005849:Gerbillinae / Q000469:parasitology; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000254:growth & development; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010767:Phosphorylcholine / Q000032:analysis*; D018510:RNA, Helminth / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D006023:Glycoproteins; D018510:RNA, Helminth; D010767:Phosphorylcholine