Mesorhizobium amorphae, a rhizobial species that nodulates Amorpha fruticosa, is native to American soils.
Amorpha fruticosa was inoculated with rhizosphere soil from Iowa, USA, and 140 rhizobia isolated from root nodules were compared with Mesorhizobium amorphae originating from Chinese soils. PCR-RFLP patterns of the 16S rRNA gene from the isolates and from M. amorphaewere the same. All isolates had a symbiotic plasmid of the same size with a single nifHgene. DNA:DNA hybridization values, DNA G+C content, and induced Nod factor patterns also were similar. We concluded that the four genotypes distinguished among 53 representative American isolates were M. amorphae. Since A. fruticosa is native to the Americas and is highly specific in its nodulation requirement, M. amorphae probably was transmitted to China.
Wang
En Tao
ET
Departmento de Microbiología, Escuala Nacional de Ciencias Biológicas, IPN, México DF, México.
Rogel
M Antonio
MA
Sui
Xin Hua
XH
Chen
Wen Xin
WX
Martínez-Romero
Esperanza
E
van Berkum
Peter
P
D002681:China; D004275:DNA, Ribosomal / Q000032:analysis; D004586:Electrophoresis / Q000379:methods; D007887:Fabaceae / Q000382:microbiology*; D007484:Iowa; D007527:Isoenzymes / Q000032:analysis; D009586:Nitrogen Fixation; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012230:Rhizobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013559:Symbiosis
D004275:DNA, Ribosomal; D007527:Isoenzymes; D012336:RNA, Ribosomal, 16S