Purification of Protein Disulfide-isomerase from Human Liver and Preparation of Its Antiserum.
Protein disulfide-isomerase has been isolated from human liver. The preparative procedure involved heat treatment, (NH(4))(2)SO(4) precipitation, CM-Sephadex C50 and DEAE-fast flow chromatography. The enzyme was homogenous and had a molecular mass of 60 kD or 120 kD as determined by sodium dodecy1 sulphate electro-phoresis and gel filtration respectively, indicating that the enzyme was a 120 kD dimmer with a subunit with molecular mass of 60 kD. The enzyme activity was as high as 830 U/g.protein as measured by the reactivation of "scrambled" ribonuclease. The antiserum of high titer was prepared by immunizing New Zealand rabbit with a mixture of the protein disulfide-isomerase and adjuvant.
Wu
Bing-Yi
BY
Zhujiang Hospital, First Military University, Guangzhou 510280, China.
Feng
Gui-Xiang
GX
Lu
Jing
J
Zhang
Fan
F
Feng
Yong-Qing
YQ
Wang
Fu-Qin
FQ