title: Action of Rubus coreanus extract on systemic and local anaphylaxis.
abstract: The effect was investigated of the aqueous extract of Rubus coreanus Miq. (Rosaceae) fruits (RCAE) on systemic and local anaphylaxis. RCAE (0.01-1 g/kg) dose-dependently inhibited systemic anaphylaxis induced by compound 48/80 in mice. RCAE (1 g/kg) also significantly inhibited local anaphylaxis activated by anti-DNP IgE. Pretreatment with RCAE at the same concentration before systemic anaphylaxis reduced the plasma histamine levels in a dose-dependent manner. RCAE (0.001-1 mg/mL) dose-dependently inhibited the histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. The level of cAMP in RPMC, when RCAE was added, significantly increased, compared with that of the normal control. Moreover, RCAE (0.01-1 mg/mL) had a significant inhibitory effect on anti-DNP IgE-induced tumour necrosis factor-alpha production from RPMC. These results indicate that RCAE may possess antianaphylactic action.
authors.affiliation: College of Pharmacy, Woosuk University, Chonju, Chonbuk, 565-701, Republic of Korea. tyshin@core.woosuk.ac.kr
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Eun-Soon
authors.initials: ES
authors.lastname: Lee
authors.forename: Dong-Ok
authors.initials: DO
authors.lastname: Eom
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry; D006632:Histamine / Q000097:blood; D006636:Histamine Release / Q000187:drug effects; D007073:Immunoglobulin E / Q000187:drug effects / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D010537:Peritoneum / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027824:Rosaceae; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D010936:Plant Extracts; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D000242:Cyclic AMP