Interferon-gamma stimulates the expression of galectin-9 in cultured human endothelial cells.
Galectin-9 is a member of the galectin family and has been identified as an eosinophil chemoattractant produced by activated T lymphocytes. Vascular endothelial cells play an important role in the initial step of eosinophil recruitment and activation in immune and inflammatory responses. We have addressed the stimulation of galectin-9 expression in endothelial cells. Galectin-9 was detected in membrane and cytosolic fractions of human umbilical vein endothelial cells stimulated with interferon-gamma (IFN-gamma). IFN-gamma also enhanced the adhesion of human eosinophilic leukemia-1 cells to endothelial monolayers, and it was inhibited by the presence of lactose. Interleukin-4, which induces eotaxin expression, did not affect the expression of galectin-9. The in situ endothelium from patients with inflammatory diseases was found to express galectin-9. IFN-gamma-induced production of galectin-9 by endothelial cells may play an important role in immune responses by regulating interactions between the vascular wall and eosinophils.
Imaizumi
Tadaatsu
T
Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, Japan. timaizum@cc.hirosaki-u.ac.jp
Kumagai
Mika
M
Sasaki
Naoko
N
Kurotaki
Hidekachi
H
Mori
Fumiaki
F
Seki
Masako
M
Nishi
Nozomu
N
Fujimoto
Koji
K
Tanji
Kunikazu
K
Shibata
Takeo
T
Tamo
Wakako
W
Matsumiya
Tomoh
T
Yoshida
Hidemi
H
Cui
Xue-Fan
XF
Takanashi
Shingo
S
Hanada
Katsumi
K
Okumura
Ken
K
Yagihashi
Soroku
S
Wakabayashi
Koichi
K
Nakamura
Takanori
T
Hirashima
Mitsuomi
M
Satoh
Kei
K
D001327:Autoimmune Diseases / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D054413:Chemokine CCL11; D019742:Chemokines, CC / Q000096:biosynthesis / Q000235:genetics; D003600:Cytosol / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D004804:Eosinophils / Q000166:cytology; D037161:Galectins; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D017681:Hypereosinophilic Syndrome / Q000473:pathology; D007371:Interferon-gamma / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology; D007785:Lactose / Q000494:pharmacology; D037102:Lectins / Q000096:biosynthesis* / Q000235:genetics; D009298:Nasal Polyps / Q000737:chemistry; D011994:Recombinant Proteins; D006255:Rhinitis, Allergic, Seasonal / Q000378:metabolism / Q000473:pathology; D012859:Sjogren's Syndrome / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects; D014471:Umbilical Veins
C515456:CCL11 protein, human; D054413:Chemokine CCL11; D019742:Chemokines, CC; D037161:Galectins; C105032:LGALS9 protein, human; D037102:Lectins; D011994:Recombinant Proteins; D015847:Interleukin-4; D007371:Interferon-gamma; D007785:Lactose