Macrophage-enhanced germination of Bacillus anthracis endospores requires gerS.
Germination of Bacillus anthracis Sterne and plasmidless Delta-Sterne endospores was dramatically enhanced in RAW264.7 macrophage-like cells, while germination of nonpathogenic Bacillus endospores was not. Elimination of gerS, a germinant receptor locus, caused a complete loss of cell-enhanced germination, implicating gerS in the breaking of endospore dormancy in vivo.
Ireland
John A W
JA
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
Hanna
Philip C
PC
D000818:Animals; D001408:Bacillus anthracis / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D002460:Cell Line; D003470:Culture Media; D005798:Genes, Bacterial; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D010587:Phagocytosis; D013171:Spores, Bacterial / Q000235:genetics / Q000502:physiology; D014774:Virulence / Q000235:genetics / Q000502:physiology
D003470:Culture Media