title: Application of recombinant fluorescent mammalian cells as a toxicity biosensor.
abstract: With respect to developing a more sensitive biosensor, a recombinant fluorescent Chinese Hamster Ovary cell line was used for the monitoring of various toxicants. Both cell lines, EFC-500 and KFC-A10, were able to detect toxicants sensitively. They were characterized with mitomycin C and gamma-ray as genotoxicants and bisphenol A, nonylphenol, ziram and methyl bromide as possible and known EDCs. When compared to each other, the response of KFC-A10 was generally more informative and sensitive. Compared to typical bacterial biosensor systems, these cell lines offered a sensitivity of 2- to 50-fold greater for the tested chemicals. Based on these results, the use of mammalian cells offers a sensitive biosensor system that is not only fast, cheap and reproducible but also capable of monitoring the endocrine-like characteristics of environmental toxicants.
authors.affiliation: Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology and the Advanced Environmental Monitoring Research Center, Puk-gu, Korea.
authors.forename: E J
authors.initials: EJ
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Lee
authors.forename: J E
authors.initials: JE
authors.lastname: Lee
authors.forename: M B
authors.initials: MB
authors.lastname: Gu
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D016466:CHO Cells; D006224:Cricetinae; D004785:Environmental Pollutants / Q000633:toxicity*; D005453:Fluorescence; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004785:Environmental Pollutants