title: Cellular effects of dopamine--beyond oxidative mechanisms.
abstract: Cell cycle blockers inhibit growth in dividing cells, but promote survival of differentiated cells, including neurons. Low micromolar dopamine profoundly inhibited cell growth in dopamine transporter transfected SK-N-MC neuroblastoma cells by cell cycle arrest at G(1). This effect was independent of oxy radical formation, antagonized by transporter block, abolished by FeCl(3) and mimicked by the iron chelator deferoxamine. We propose that dopamine inhibits cell growth by its ability to chelate intracellular iron. This novel biological action unrelated to neurotransmitter receptors, second messengers or oxidative stress, observed in human neuroblastoma cells of striatal origin, may be important for cell differentiation during neurodevelopment and survival of differentiated dopamine (nigral) neurons.
authors.affiliation: Brain Research Institute, University of Vienna, Spitalgasse 4, 1090, Vienna, Austria. christian.pifl@univie.ac.at
authors.forename: Christian
authors.initials: C
authors.lastname: Pifl
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kattinger
authors.forename: Harald
authors.initials: H
authors.lastname: Reither
authors.forename: Oleh
authors.initials: O
authors.lastname: Hornykiewicz
mesh_terms: D002452:Cell Count / Q000379:methods; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D004298:Dopamine / Q000378:metabolism / Q000502:physiology*; D050483:Dopamine Plasma Membrane Transport Proteins; D006801:Humans; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D018076:DNA, Complementary; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D016877:Oxidants; D004298:Dopamine