Rapid alterations of serum oxidant and antioxidant status with the intravenous administration of n-6 polyunsaturated fatty acids.
In an attempt to achieve the safe intravenous administration of two n-6 polyunsaturated fatty acids (PUFAs), gamma-linolenic acid (GLA) and arachidonic acid (AA), and to study the subsequent changes on the total oxidant and antioxidant status, various steadily increasing doses of each acid were injected intravenously at different infusion times in 28 male rabbits. Blood samples were collected at 15-min time intervals by the hepatic veins and from the carotid artery; oxidant status was determined by the thiobarbiturate assay and total antioxidant status (TAS) was assessed by a colorimetric assay. Both n-6 PUFAs were administered with safety at a dose of 25 mg/kg within 10 min accompanied by an increase of malonodialdehyde concentrations in the hepatic veins and in the carotid artery 30-45 min, respectively, after the end of the infusion of GLA and/or AA. Similar changes did not occur in red cell membranes after the infusion of AA. TAS presented reciprocal changes to malonodialdehyde production; the main consumption of TAS was observed in all samples 30-60 min after the end of the infusion of n-6 PUFAs. The above-mentioned rapid alterations occurring in both serum oxidant and antioxidant status after GLA might have a future clinical therapeutic significance in conditions like cancer and disseminated infectious diseases.
Giamarellos-Bourboulis
E J
EJ
4th Department of Internal Medicine, Medical School, University of Athens, Greece. giamarel@internet.gr
Skiathitis
S
S
Dionyssiou-Asteriou
A
A
Donta
I
I
Hatziantoniou
S
S
Demetzos
K
K
Papaioannou
G Th
GT
Karatzas
G
G
Giamarellou
H
H
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001774:Blood Chemical Analysis; D002339:Carotid Arteries / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage* / Q000494:pharmacology*; D006503:Hepatic Veins / Q000187:drug effects / Q000378:metabolism; D007275:Injections, Intravenous; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D016877:Oxidants / Q000097:blood*; D011817:Rabbits
D000975:Antioxidants; D043371:Fatty Acids, Omega-6; D016877:Oxidants; D008315:Malondialdehyde