title: Characterization of 11 human sarcoma cell strains: evaluation of cytogenetics, tumorigenicity, metastasis, and production of angiogenic factors.
abstract: BACKGROUND
  Human sarcomas have a propensity for aggressive local invasion and early pulmonary metastasis. Frequently, deaths are due to uncontrolled pulmonary metastases. The purpose of the current study was to evaluate cytogenetics, tumorigenicity, metastatic potential, and production of angiogenic factors in human sarcoma cell strains. A secondary purpose was to establish low passage cell strains for studying new therapeutic approaches.
  
  
  METHODS
  The authors established 11 cell strains from human sarcoma surgical specimens and characterized their in vitro tumor properties, including growth in soft agar, expression of angiogenic growth factors (vascular endothelial growth factor [VEGF] and basic-fibroblast growth factor [bFGF]), and cytogenetics.
  
  
  RESULTS
  All of the cell strains remained diploid. All exhibited the ability to grow in soft agar and expressed both VEGF as well as bFGF. In addition, 6 of the 11 established sarcoma cell strains were tumorigenic, 5 of which spontaneously metastasized to the lungs in nude mice. Four of the five cell strains that yielded lung metastases were derived from lung metastases in patients.
  
  
  CONCLUSIONS
  The 11 cell strains, which were derived from diverse sarcoma histologies, will provide a model for studying not only metastatic progression but also the in vitro and in vivo efficacy of new therapeutic modalities for human sarcomas.
authors.affiliation: Division of Surgical Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Hu
authors.forename: Garth L
authors.initials: GL
authors.lastname: Nicolson
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Trent
authors.forename: Dihua
authors.initials: D
authors.lastname: Yu
authors.forename: Lianglin
authors.initials: L
authors.lastname: Zhang
authors.forename: Aiqing
authors.initials: A
authors.lastname: Lang
authors.forename: Ann
authors.initials: A
authors.lastname: Killary
authors.forename: Lee M
authors.initials: LM
authors.lastname: Ellis
authors.forename: Corazon D
authors.initials: CD
authors.lastname: Bucana
authors.forename: Raphael E
authors.initials: RE
authors.lastname: Pollock
mesh_terms: D000818:Animals; D016228:Endothelial Growth Factors / Q000096:biosynthesis; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D006801:Humans; D008222:Lymphokines / Q000096:biosynthesis; D051379:Mice; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental; D009389:Neovascularization, Pathologic; D011003:Ploidies; D012509:Sarcoma / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2