[Rapid high performance liquid chromatography for determination of phenylalanine and tyrosine in serum].
OBJECTIVE
To develop a rapid assay for the determination of phenylalanine and tyrosine in serum.


METHODS
The serum sample was injected directly into High performance liquid chromatography(HPLC) system after precipitation of protein with perchloric acid, and monitored at 190 nm with an ultraviolet detector.


RESULTS
Good linearity was observed in 6-1,512 mumol.L-1 range for phenylalanine, the intra-run and inter-run imprecisions(CV) were 3.68% and 4.01%, the recovery rate was 98.6%; good linearity was observed in 5.4-1,380.0 mumol.L-1 range for tyrosine, the intra-run and inter-run imprecisions were 1.87% and 2.59%, the average recovery rate was 98.9%.


CONCLUSIONS
This method is simple, rapid, sensitive, accurate, and suitable for clinical application.
Tang
A G
AG
Department of Clinical Laboratory, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
D000328:Adult; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010649:Phenylalanine / Q000097:blood*; D010661:Phenylketonurias / Q000097:blood*; D012016:Reference Values; D014443:Tyrosine / Q000097:blood*
D014443:Tyrosine; D010649:Phenylalanine