Concentration gradient used in double-stranded DNA separation by capillary electrophoresis.
Effects of concentration gradient on double-stranded DNA (dsDNA) separation by capillary electrophoresis are presented. By using a concentration gradient in the range between 0.8% and 3.2% for poly(N,N-dimethylacrylamide) (PDMA), the presence of a mesh-size gradient in the capillary could enhance the separation of larger size DNA fragments, better than that based on a single uniform concentration over the same capillary length. A decrease in the column length could make the gradient effect more obvious. An optimal capillary length could be achieved by using a judicious combination of the concentration gradient and the concentration range, yielding a maximum resolution for the system. The standard deviation of the migration time measured for each DNA fragment was less than 5% in ten continuous runs, suggesting that the gradient formed inside the column was quite stable.
Liang
Dehai
D
Department of Chemistry, State University of New York at Stony Brook, Stony Brook, NY 11794-3400, USA.
Chu
Benjamin
B
D000178:Acrylamides; D004247:DNA / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000178:Acrylamides; C429790:poly(N,N-dimethylacrylamide); D004247:DNA