[Frequency of prothrombin gene G20210A variant in the 3'-untranslated region in Chinese people with ischemic stroke].
OBJECTIVE
To ascertain the frequency of prothrombin(F II) gene 3'-untranslated region(3'-UT) G20210A variant and to explore whether this mutation is related to arterial thrombosis in Chinese people.


METHODS
We studied 49 patients with thrombosis and 46 healthy Chinese persons. The patients had a first ischemic stroke. The G20210A mutant allele of the prothrombin gene in all blood specimens was investigated by DNA extraction, polymerase chain reaction amplification, Hind III digestion and polyacrylamide gel electrophoresis (PAGE).


RESULTS
The patients and normal control subjects were all homozygous for the normal G20210G allele, and there was no any F II G20210A variant.


CONCLUSIONS
Factor II gene 3'-UT G20210A mutant allele is absent in Chinese patients with ischemic stroke and normal subjects; its mutation may not be a major risk factor for thrombogenesis in Chinese people.
Chang
J H
JH
Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
Zhang
G S
GS
D020413:3' Untranslated Regions / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D020520:Brain Infarction / Q000097:blood / Q000235:genetics*; D005260:Female; D006801:Humans; D002546:Ischemic Attack, Transient / Q000097:blood / Q000235:genetics; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D011516:Prothrombin / Q000235:genetics*
D020413:3' Untranslated Regions; D011516:Prothrombin