title: A selective effect of Mpl ligand on mRNA stabilization during megakaryocyte differentiation.
abstract: Megakaryocytes, the platelet precursors, are induced to differentiate in response to Mpl ligand. Here we report that stability of the megakaryocyte-specific platelet factor 4 (PF4) mRNA is substantially augmented in the presence of Mpl ligand. This stabilization requires protein synthesis, but the 3'-untranslated region of PF4 mRNA is not sufficient for granting the effect. This cytokine also significantly or mildly stabilizes Mpl receptor or GAPDH mRNAs, respectively, in contrast to a previously reported lack of effect on P2Y(1) receptor mRNA. Our study is the first to suggest that Mpl ligand-induced lineage specification is also determined by message stabilization.
authors.affiliation: Department of Biochemistry, K225, Boston University School of Medicine, 715 Albany St., Boston, MA 02118, USA.
authors.forename: Yulia
authors.initials: Y
authors.lastname: Kaluzhny
authors.forename: Beatrice
authors.initials: B
authors.lastname: Hechler
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.forename: Hao G
authors.initials: HG
authors.lastname: Nguyen
authors.forename: Leah M
authors.initials: LM
authors.lastname: Cataldo
authors.forename: Katya
authors.initials: K
authors.lastname: Ravid
mesh_terms: D024509:3' Flanking Region; D020413:3' Untranslated Regions; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000187:drug effects / Q000235:genetics; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics; D010978:Platelet Factor 4 / Q000187:drug effects / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics; D020871:RNA Stability; D012333:RNA, Messenger / Q000187:drug effects*; D018121:Receptors, Cytokine / Q000235:genetics; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000235:genetics; D058472:Receptors, Purinergic P2Y1; D053628:Receptors, Thrombopoietin; D013926:Thrombopoietin / Q000494:pharmacology*
chemical_list: D020413:3' Untranslated Regions; D009363:Neoplasm Proteins; C546382:P2RY1 protein, human; C546383:P2ry1 protein, mouse; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D018048:Receptors, Purinergic P2; D058472:Receptors, Purinergic P2Y1; D053628:Receptors, Thrombopoietin; C082218:MPL protein, human; D010978:Platelet Factor 4; D013926:Thrombopoietin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases