title: Analysis of the reg1alpha and reg1beta gene transcripts in patients with fibrocalculous pancreatopathy.
abstract: Fibrocalculous pancreatopathy is a form of diabetes, associated with tropical chronic calcific pancreatitis, in which islet beta-cell loss and pancreatic stone formation are found. It is likely to be a multifactorial disease with both genetic and environmental components. Regenerating (reg) gene encodes protein that has been involved in pancreatic lithogenesis and the regeneration of islet cells and therefore the abnormality of reg genes could be associated with fibrocalculous pancreatopathy. In this study, regla and reg1beta mRNAs were isolated from peripheral blood lymphocytes obtained from 16 patients with fibrocalculous pancreatopathy, 42 patients with type 1 diabetes, 37 patients with type 2 diabetes, and 22 normal controls. mRNAs were amplified by reverse-transcription polymerase chain reaction (RT-PCR) and analysed by a single strand conformation polymorphism (SSCP) technique. The reg1alpha and reg1beta mRNAs were isolated, indicating the ectopic expression of these genes in peripheral blood lymphocytes; however, variation among mobility patterns was not observed in the SSCP analysis of the RT-PCR products. The results indicated that there was no abnormality of the regla and reg1beta mRNAs obtained from the study groups.
authors.affiliation: Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Watip
authors.initials: W
authors.lastname: Boonyasrisawat
authors.forename: Pinya
authors.initials: P
authors.lastname: Pulsawat
authors.forename: Pa-Thai
authors.initials: PT
authors.lastname: Yenchitsomanus
authors.forename: Sathit
authors.initials: S
authors.lastname: Vannasaeng
authors.forename: Pakom
authors.initials: P
authors.lastname: Pramukkul
authors.forename: Chaicharn
authors.initials: C
authors.lastname: Deerochanawong
authors.forename: Sutin
authors.initials: S
authors.lastname: Sriussadaporn
authors.forename: Sirirat
authors.initials: S
authors.lastname: Ploybutr
authors.forename: Thawatchai
authors.initials: T
authors.lastname: Pasurakul
authors.forename: Napatawn
authors.initials: N
authors.lastname: Banchuin
mesh_terms: D002135:Calcium-Binding Proteins / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006801:Humans; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D010182:Pancreatic Diseases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000235:genetics*; D013785:Thailand
chemical_list: D002135:Calcium-Binding Proteins; D051847:Lithostathine; D009419:Nerve Tissue Proteins; C497617:REG1A protein, human; C497618:REG1B protein, human; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes