The responsiveness of human V delta 1 gamma delta T cells to Borrelia burgdorferi is largely restricted to synovial-fluid cells from patients with Lyme arthritis.
It has been shown that human gamma delta T cells expressing the V delta 1 T cell receptor (TCR) respond to the spirochete Borrelia burgdorferi. Lysates of 3 Borrelia genospecies triggered the proliferation of peripheral-blood mononuclear cells not only from patients with skin manifestations of Borrelia infection and from patients with Lyme arthritis but also from healthy donors. However, with the exception of 1 patient with Lyme arthritis, no selective expansion of V delta 1-expressing gamma delta T cells was induced. In contrast, synovial-fluid mononuclear cells (SFMC) from 3 of 5 patients with Lyme arthritis responded with a selective outgrowth of V delta 1 gamma delta T cells. V delta 1 gamma delta T cell lines established from SFMC coexpressed various TCR V gamma chains, although V gamma 8 was preferentially used. Thus, the responsiveness to Borrelia antigens is not a general property of V delta 1-expressing gamma delta T cells but is largely restricted to V delta 1 gamma delta T cells recruited into the inflamed tissue.
Glatzel
Andrea
A
Institute of Immunology, University of Kiel, Kiel, Germany.
Entschladen
Frank
F
Zollner
Thomas M
TM
Kraiczy
Peter
P
Brade
Volker
V
Kaufmann
Roland
R
Janssen
Ottmar
O
Lengl-Janssen
Beate
B
Wesch
Daniela
D
Kabelitz
Dieter
D
D000293:Adolescent; D000328:Adult; D000368:Aged; D001170:Arthritis, Infectious / Q000276:immunology*; D025065:Borrelia burgdorferi / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008193:Lyme Disease / Q000276:immunology*; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D013582:Synovial Fluid / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D016692:Receptors, Antigen, T-Cell, gamma-delta