title: The effect of in vivo mechanical loading on estrogen receptor alpha expression in rat ulnar osteocytes.
abstract: The presence of estrogen receptor alpha (ER alpha) in osteocytes was identified immunocytochemically in transverse sections from 560 to 860 microm distal to the midshaft of normal neonatal and adult male and female rat ulnas (n = 3 of each) and from adult male rat ulnas that had been exposed to 10 days of in vivo daily 10-minute periods of cyclic loading producing peak strains of either -3000 (n = 3) or -4000 microstrain (n = 5). Each animal ambulated normally between loading periods, and its contralateral ulna was used as a control. In animals in which limbs were subject to normal locomotor loading alone, 14 +/- 1.2% SEM of all osteocytes in each bone section were ER alpha positive. There was no influence of either gender (p = 0.725) or age (p = 0.577) and no interaction between them (p = 0.658). In bones in which normal locomotion was supplemented by short periods of artificial loading, fewer osteocytes expressed ER alpha (7.5 +/- 0.91% SEM) than in contralateral control limbs, which received locomotor loading alone (14 +/- 1.68% SEM; p = 0.01; median difference, 6.43; 95% CI, 2.60, 10.25). The distribution of osteocytes expressing ER alpha was uniform across all sections and thus did not reflect local peak strain magnitude. This suggests that osteocytes respond to strain as a population, rather than as individual strain-responsive cells. These data are consistent with the hypothesis that ER alpha is involved in bone cells' responses to mechanical strain. High strains appear to decrease ER alpha expression. In osteoporotic bone, the high strains assumed to accompany postmenopausal bone loss may reduce ER alpha levels and therefore impair the capacity for appropriate adaptive remodeling.
authors.affiliation: Department of Veterinary Basic Sciences, The Royal Veterinary College, London, United Kingdom.
authors.forename: P J
authors.initials: PJ
authors.lastname: Ehrlich
authors.forename: B S
authors.initials: BS
authors.lastname: Noble
authors.forename: H L
authors.initials: HL
authors.lastname: Jessop
authors.forename: H Y
authors.initials: HY
authors.lastname: Stevens
authors.forename: J R
authors.initials: JR
authors.lastname: Mosley
authors.forename: L E
authors.initials: LE
authors.lastname: Lanyon
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001696:Biomechanical Phenomena; D047628:Estrogen Receptor alpha; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D010011:Osteocytes / Q000378:metabolism*; D015663:Osteoporosis, Postmenopausal / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000378:metabolism*; D013314:Stress, Mechanical; D014457:Ulna / Q000166:cytology / Q000378:metabolism / Q000502:physiology
chemical_list: D047628:Estrogen Receptor alpha; D011960:Receptors, Estrogen