title: A bovine whole-genome radiation hybrid panel and outline map.
abstract: A 3000-rad radiation hybrid panel was constructed for cattle and used to build outline RH maps for all 29 autosomes and the X and Y chromosomes. These outline maps contain about 1200 markers, most of which are anonymous microsatellite loci. Comparisons between the RH chromosome maps, other published RH maps, and linkage maps allow regions of chromosomes that are poorly mapped or that have sparse marker coverage to be identified. In some cases, mapping ambiguities can be resolved. The RH maps presented here are the starting point for mapping additional loci, in particular genes and ESTs that will allow detailed comparative maps between cattle and other species to be constructed. Radiation hybrid cell panels allow high-density genetic maps to be constructed, with the advantage over linkage mapping that markers do not need to be polymorphic. A large quantity of DNA has been prepared from the cells forming the RH panel reported here and is publicly available for mapping large numbers of loci.
authors.affiliation: Roslin Institute (Edinburgh), Roslin, Midlothian, Scotland EH25 9PS, United Kingdom. John.Williams@BBSRC.AC.UK
authors.forename: John L
authors.initials: JL
authors.lastname: Williams
authors.forename: André
authors.initials: A
authors.lastname: Eggen
authors.forename: Luca
authors.initials: L
authors.lastname: Ferretti
authors.forename: Christine J
authors.initials: CJ
authors.lastname: Farr
authors.forename: Mathieu
authors.initials: M
authors.lastname: Gautier
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Amati
authors.forename: Glynn
authors.initials: G
authors.lastname: Ball
authors.forename: Tiziana
authors.initials: T
authors.lastname: Caramorr
authors.forename: Ricky
authors.initials: R
authors.lastname: Critcher
authors.forename: Sandro
authors.initials: S
authors.lastname: Costa
authors.forename: Patrick
authors.initials: P
authors.lastname: Hextall
authors.forename: David
authors.initials: D
authors.lastname: Hills
authors.forename: Aurore
authors.initials: A
authors.lastname: Jeulin
authors.forename: Susanna L
authors.initials: SL
authors.lastname: Kiguwa
authors.forename: Olivia
authors.initials: O
authors.lastname: Ross
authors.forename: Angela L
authors.initials: AL
authors.lastname: Smith
authors.forename: Katiana
authors.initials: K
authors.lastname: Saunier
authors.forename: Barbara
authors.initials: B
authors.lastname: Urquhart
authors.forename: Dave
authors.initials: D
authors.lastname: Waddington
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D005819:Genetic Markers; D016678:Genome; D008297:Male; D018895:Microsatellite Repeats; D021101:Radiation Hybrid Mapping
chemical_list: D005819:Genetic Markers