Genomic and phylogenetic analyses of an adenovirus isolated from a corn snake (Elaphe guttata) imply a common origin with members of the proposed new genus Atadenovirus.
Approximately 60% of the genome of an adenovirus isolated from a corn snake (Elaphe guttata) was cloned and sequenced. The results of homology searches showed that the genes of the corn snake adenovirus (SnAdV-1) were closest to their counterparts in members of the recently proposed new genus ATADENOVIRUS: In phylogenetic analyses of the complete hexon and protease genes, SnAdV-1 indeed clustered together with the atadenoviruses. The characteristic features in the genome organization of SnAdV-1 included the presence of a gene homologous to that for protein p32K, the lack of structural proteins V and IX and the absence of homologues of the E1A and E3 regions. These characteristics are in accordance with the genus-defining markers of atadenoviruses. Comparison of the cleavage sites of the viral protease in core protein pVII also confirmed SnAdV-1 as a candidate member of the genus ATADENOVIRUS: Thus, the hypothesis on the possible reptilian origin of atadenoviruses (Harrach, Acta Veterinaria Hungarica 48, 484-490, 2000) seems to be supported. However, the base composition of DNA sequence (>18 kb) determined from the SnAdV-1 genome showed an equilibrated GC content of 51%, which is unusual for an atadenovirus.
Farkas
Szilvia L
SL
Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary1.
Benkő
Mária
M
Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary1.
Élő
Péter
P
Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary1.
Ursu
Krisztina
K
Central Veterinary Institute, PO Box 2, H-1581 Budapest, Hungary2.|Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary1.
Dán
Ádám
Á
Central Veterinary Institute, PO Box 2, H-1581 Budapest, Hungary2.|Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary1.
Ahne
Winfried
W
Institute for Zoology, Fish Biology, Fish Diseases, University of München, Germany3.
Harrach
Balázs
B
Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary1.
D000256:Adenoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017500:Adenovirus E1A Proteins / Q000235:genetics; D017503:Adenovirus E3 Proteins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000378:metabolism; D036022:Capsid Proteins; D017812:Colubridae / Q000821:virology*; D004279:DNA, Viral; D010450:Endopeptidases / Q000378:metabolism; D019143:Evolution, Molecular; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D014758:Viral Core Proteins / Q000378:metabolism
D017500:Adenovirus E1A Proteins; D017503:Adenovirus E3 Proteins; D036022:Capsid Proteins; D004279:DNA, Viral; D014758:Viral Core Proteins; C018315:hexon capsid protein, Adenovirus; C090876:polypeptide VI, adenovirus; D010450:Endopeptidases