PCR-RFLP based molecular typing of enteroviruses isolated from patients with aseptic meningitis in Korea.
We have evaluated PCR-RFLP as a practical method for rapid typing of enteroviruses causing aseptic meningitis in Korea. Through blind examination of 80 clinical isolates from patients with aseptic meningitis, we have compared the results of conventional serotyping with PCR-RFLP based genotyping, which was developed for this study. Among the 80 case isolates, which had been previously typed by routine neutralization test, only 42 cases (52.5%) were matched with typing by PCR-RFLP. The result clearly demonstrated that the enterovirus serotype does not coincide with the genotype. Therefore, the classification of enteroviruses by genotyping with PCR-RFLP, although rapid and simple, may be complicated by regional or seasonal differences. However, the PCR-RFLP method developed in this study is applicable to the epidemiological study of enteroviruses when regional or seasonal differences exist, and is useful in identifying the source of an infection.
Lee
Y S
YS
Department of Life Science, College of Natural Science, Chung-Ang University, Seoul, Korea.
Kim
K S
KS
Chung
Y S
YS
Cheon
D S
DS
Jung
S K
SK
Park
K S
KS
Na
B K
BK
Jee
Y M
YM
Kim
D S
DS
Yoon
J D
JD
Song
C Y
CY
Lee
K H
KH
D004770:Enterovirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D005838:Genotype; D006801:Humans; D008582:Meningitis, Aseptic / Q000821:virology*; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping