title: Expression of ATP1AL1, a non-gastric proton pump, in human colorectum.
abstract: The expression of mRNAs encoding a human nongastric proton pump (ATP1AL1) in the colorectum was investigated. The real-time PCR gave significant levels of signals not only in the distal part of human colon and rectum, but also in the proximal part of the colon. ATP1AL1 mRNA was overexpressed in 12 out of 20 human colorectal adenocarcinomas compared with the level in the accompanying normal mucosa. It is noted that astonishing levels of the mRNA overexpression were found in 4 carcinomas, which were detected even by Northern blot. The very high levels of ATP1AL1 mRNA expression in some cancer tissues may be connected to an unknown specific pathophysiological condition.
authors.affiliation: Department of Pharmaceutical Physiology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Toyama, 930-0194 Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Hideki
authors.initials: H
authors.lastname: Sakai
authors.forename: Mutsuko
authors.initials: M
authors.lastname: Kuragari
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Suzuki
authors.forename: Katsunori
authors.initials: K
authors.lastname: Tauchi
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Minamimura
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tsukada
authors.forename: Shinji
authors.initials: S
authors.lastname: Asano
authors.forename: Noriaki
authors.initials: N
authors.lastname: Takeguchi
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism; D000818:Animals; D002277:Carcinoma / Q000378:metabolism; D003106:Colon / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism; D003199:Computer Systems; D017506:H(+)-K(+)-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction; D017494:Proton Pumps / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012007:Rectum / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D017494:Proton Pumps; D012333:RNA, Messenger; C088641:ATP12A protein, human; D017506:H(+)-K(+)-Exchanging ATPase; D000254:Sodium-Potassium-Exchanging ATPase