Preparation and sensitization of polystyrene latex beads by some antigens and antibodies. Factors affecting sensitivity and specificity of latex agglutination tests.
Polystyrene latex beads were polymerized at two different pH (7.3 and 9.5) and their ability to create latex agglutination systems were studied. Sensitization of these latexes by DNA, antibodies to C-reactive protein, myoglobin, and human IgG. was conducted. Sensitivity and specificity of these systems were compared. Practical recommendations are given for preparation of such systems.
Gasparyan
V K
VK
H. Ch. Buniatyan Institute of Biochemistry of the Armenian NAS, Yerevan, Republic of Armenia. biochem@ipia.sci.am
D000818:Animals; D000906:Antibodies; D000941:Antigens; D002097:C-Reactive Protein / Q000276:immunology; D002417:Cattle; D004247:DNA; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007074:Immunoglobulin G / Q000276:immunology; D007841:Latex Fixation Tests / Q000592:standards*; D008863:Microspheres; D009211:Myoglobin / Q000276:immunology; D011137:Polystyrenes / Q000138:chemical synthesis* / Q000592:standards; D011485:Protein Binding; D011817:Rabbits; D012680:Sensitivity and Specificity
D000906:Antibodies; D000941:Antigens; D007074:Immunoglobulin G; D009211:Myoglobin; D011137:Polystyrenes; C024075:styrofoam; D002097:C-Reactive Protein; D004247:DNA