title: Expression of cadherin-8 in renal cell carcinoma and fetal kidney.
abstract: Cadherins represent a family of calcium-dependent cell adhesion molecules with an important regulatory function for maintenance of tissue architecture. Alterations of cadherin expression have been demonstrated in the development and progression of different epithelial tumors. In renal cell carcinoma (RCC), the majority of tumors express N-cadherin and cadherin-6. Screening a series of 16 RCC cell lines for the expression of different novel type II cadherins by RT-PCR revealed a complex pattern of cadherin expression: cadherins 6 and 14 were expressed in most of the RCC cell lines, whereas cadherins 11, 12 and 13 could not be detected at all. Interestingly, cadherin-8, previously shown in mice to be restricted to the CNS and thymus during development, was detected by RT-PCR, immunofluorescence and in situ hybridization in 4 of 16 RCC cell lines as well as in paraffin sections of the corresponding human RCC biopsies. In normal renal tissue, however, cadherin-8 could be detected only during the early stages of kidney development. These results suggest that alterations of type II cadherin expression may play a role in RCC development. In particular, cadherin-8 may be involved in both kidney morphogenesis as well as tumorigenesis in some types of RCC.
authors.affiliation: Department of Nephrology and Rheumatology, Georg-August University, Göttingen, Germany. sblasch@gwdg.de
authors.forename: Sabine
authors.initials: S
authors.lastname: Blaschke
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Mueller
authors.forename: Jasmina
authors.initials: J
authors.lastname: Markovic-Lipkovski
authors.forename: Sabine
authors.initials: S
authors.lastname: Puch
authors.forename: Nicolai
authors.initials: N
authors.lastname: Miosge
authors.forename: Volker
authors.initials: V
authors.lastname: Becker
authors.forename: Gerhard A
authors.initials: GA
authors.lastname: Mueller
authors.forename: Gerd
authors.initials: G
authors.lastname: Klein
mesh_terms: D001706:Biopsy; D015153:Blotting, Western; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007668:Kidney / Q000196:embryology* / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism*; D009024:Morphogenesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: C112316:CDH8 protein, human; D015820:Cadherins