Analysis of the reg1alpha and reg1beta gene transcripts in patients with fibrocalculous pancreatopathy.
Fibrocalculous pancreatopathy is a form of diabetes, associated with tropical chronic calcific pancreatitis, in which islet beta-cell loss and pancreatic stone formation are found. It is likely to be a multifactorial disease with both genetic and environmental components. Regenerating (reg) gene encodes protein that has been involved in pancreatic lithogenesis and the regeneration of islet cells and therefore the abnormality of reg genes could be associated with fibrocalculous pancreatopathy. In this study, regla and reg1beta mRNAs were isolated from peripheral blood lymphocytes obtained from 16 patients with fibrocalculous pancreatopathy, 42 patients with type 1 diabetes, 37 patients with type 2 diabetes, and 22 normal controls. mRNAs were amplified by reverse-transcription polymerase chain reaction (RT-PCR) and analysed by a single strand conformation polymorphism (SSCP) technique. The reg1alpha and reg1beta mRNAs were isolated, indicating the ectopic expression of these genes in peripheral blood lymphocytes; however, variation among mobility patterns was not observed in the SSCP analysis of the RT-PCR products. The results indicated that there was no abnormality of the regla and reg1beta mRNAs obtained from the study groups.
Boonyasrisawat
Watip
W
Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Pulsawat
Pinya
P
Yenchitsomanus
Pa-Thai
PT
Vannasaeng
Sathit
S
Pramukkul
Pakom
P
Deerochanawong
Chaicharn
C
Sriussadaporn
Sutin
S
Ploybutr
Sirirat
S
Pasurakul
Thawatchai
T
Banchuin
Napatawn
N
D002135:Calcium-Binding Proteins / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006801:Humans; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D010182:Pancreatic Diseases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000235:genetics*; D013785:Thailand
D002135:Calcium-Binding Proteins; D051847:Lithostathine; D009419:Nerve Tissue Proteins; C497617:REG1A protein, human; C497618:REG1B protein, human; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes