Linker histone subtype composition and affinity for chromatin in situ in nucleated mature erythrocytes.
The replacement linker histones H1(0) and H5 are present in frog and chicken erythrocytes, respectively, and their accumulation coincides with cessation of proliferation and compaction of chromatin. These cells have been analyzed for the affinity of linker histones for chromatin with cytochemical and biochemical methods. Our results show a stronger association between linker histones and chromatin in chicken erythrocyte nuclei than in frog erythrocyte nuclei. Analyses of linker histones from chicken erythrocytes using capillary electrophoresis showed H5 to be the subtype strongest associated with chromatin. The corresponding analyses of frog erythrocyte linker histones using reverse-phase high performance liquid chromatography showed that H1(0) dissociated from chromatin at somewhat higher ionic strength than the three additional subtypes present in frog blood but at lower ionic strength than chicken H5. Which of the two H1(0) variants in frog is expressed in erythrocytes has thus far been unknown. Amino acid sequencing showed that H1(0)-2 is the only H1(0) subtype present in frog erythrocytes and that it is 100% acetylated at its N termini. In conclusion, our results show differences between frog and chicken linker histone affinity for chromatin probably caused by the specific subtype composition present in each cell type. Our data also indicate a lack of correlation between linker histone affinity and chromatin condensation.
Koutzamani
Elisavet
E
Department of Biomedicine and Surgery, Division of Cell Biology, Faculty of Health Sciences, Linköpings universitet, SE-581 85 Linköping, Sweden.
Loborg
Helena
H
Sarg
Bettina
B
Lindner
Herbert H
HH
Rundquist
Ingemar
I
D000818:Animals; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D002645:Chickens; D002843:Chromatin / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004072:Digitonin / Q000494:pharmacology; D004900:Erythroblasts / Q000187:drug effects / Q000502:physiology*; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007202:Indicators and Reagents / Q000494:pharmacology; D008856:Microscopy, Fluorescence / Q000379:methods; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012965:Sodium Chloride / Q000378:metabolism; D014982:Xenopus laevis
D002843:Chromatin; D006657:Histones; D007202:Indicators and Reagents; D012965:Sodium Chloride; D004072:Digitonin