Factors influencing cDNA microarray hybridization on silylated glass slides.
cDNA microarray technology is becoming the technique of choice for studying gene expression and gene expression patterns. Although experimental protocols are available, only limited methodological information on microarray manufacture, hybridization, and signal interpretation has been published. The aim of this paper is to provide more insight into the practical aspects of microarray construction and hybridization. The influence of the size, composition, and concentration of the spotted DNA fragments on the final hybridization signal and the effect of hybridization volume, sample concentration, and sample depletion have been tested and are discussed.
Franssen-van Hal
Nicole L W
NL
RIKILT (State Institute for Quality Control of Agricultural Products), Gene Expression and Detection Centre, P.O. Box 230, 6700 AE Wageningen, The Netherlands. n.l.w.franssen@rikilt.wag-ur.nl
Vorst
Oscar
O
Kramer
Evelien
E
Hall
Robert D
RD
Keijer
Jaap
J
D000588:Amines / Q000737:chemistry; D001482:Base Composition; D018938:Caco-2 Cells; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005822:Genetic Vectors / Q000378:metabolism; D005898:Glass / Q000737:chemistry*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D032306:Petunia / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry*; D013379:Substrate Specificity
D000588:Amines; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D012821:Silanes; D011061:Poly A; D008156:Luciferases