title: Visualization of translated tau protein in the axons of neuronal P19 cells and characterization of tau RNP granules.
abstract: Localization of tau mRNA to the axon requires the axonal localization cis signal (ALS), which is located within the 3' untranslated region, and trans-acting binding proteins, which are part of the observed granular structures in neuronal cells. In this study, using both biochemical and morphological methods, we show that the granules contain tau mRNA, HuD RNA-binding protein, which stabilizes mRNA, and KIF3A, a member of the kinesin microtubule-associated motor protein family involved in anterograde transport. The granules are detected along the axon and accumulate in the growth cone. Inhibition of KIF3A expression caused neurite retraction and inhibited tau mRNA axonal targeting. Taken together, these results suggest that HuD and KIF3A proteins are present in the tau mRNA axonal granules and suggest an additional function for the kinesin motor family in the microtubule-dependent translocation of RNA granules. Localized tau-GFP expression was blocked by a protein synthesis inhibitor, and upon release from inhibition, nascent tau-GFP 'hot spots' were directly observed in the axon and growth cones. These observations are consistent with local protein synthesis in the axon resulting from the transported tau mRNA.
authors.affiliation: Department of Neurobiology, The Weizmann Institute of Science, Rehovot, 76100 Israel.
authors.forename: Stella
authors.initials: S
authors.lastname: Aronov
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Aranda
authors.forename: Leah
authors.initials: L
authors.lastname: Behar
authors.forename: Irith
authors.initials: I
authors.lastname: Ginzburg
mesh_terms: D000818:Animals; D016375:Antisense Elements (Genetics); D001370:Axonal Transport / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000235:genetics / Q000378:metabolism; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; D049452:Green Fluorescent Proteins; D020439:Growth Cones / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D016547:Kinesins / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins; D014411:Neoplastic Stem Cells; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics; D021381:Protein Transport / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins / Q000378:metabolism*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism; D016875:tau Proteins / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D016375:Antisense Elements (Genetics); D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; C498010:ELAVL4 protein, human; C087415:KIF3A protein, human; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins; D014404:Tubulin; D016875:tau Proteins; D049452:Green Fluorescent Proteins; D016547:Kinesins