title: [Simultaneous determination of N-methylcarbamate pesticides and their metabolites in agricultural products].
abstract: We investigated the determination of N-methylcarbamate pesticides and their metabolites in agricultural products by HPLC with post-column fluorescence detection after clean-up with an SPE cartridge. The homogenate of agricultural products was extracted with acetone. The crude extract was partitioned between 5% sodium chloride solution and dichloromethane, and the dichloromethane layer was concentrated to dryness. The residue was dissolved with a mixture of acetone and n-hexane, and purified by using Supelclean ENVI-Carb SPE, Bond Elut Extraction Cartridge PSA and SAX in series with a mixture of acetone and n-hexane. N-Methylcarbamate pesticide was analyzed by HPLC with post-column reaction and fluoresce detection. N-Methylcarbamate pesticide in citrus fruits and various kinds of agricultural products could be analyzed accurately by the presented method. Recoveries of N-methylcarbamate pesticides added to several agricultural products at the level of 0.10 ppm were mostly in the range of 60-110%. The limit of detection was 0.005 ppm.
authors.affiliation: Tokyo Metropolitan Research Laboratory of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nagayama
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takano
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tamura
authors.forename: Naoko
authors.initials: N
authors.lastname: Kimura
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kitayama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Saito
mesh_terms: D002219:Carbamates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D018556:Crops, Agricultural / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006540:Herbicides / Q000032:analysis*; D007306:Insecticides / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*
chemical_list: D002219:Carbamates; D006540:Herbicides; D007306:Insecticides; D010573:Pesticide Residues; C020401:N-methylcarbamate