[A study on digestion of Gal alpha (1,3) Gal on porcine vascular endothelial cells by alpha-galactosidase].
This study was designed to explore ways to prevent immune rejection in xenotransplantation. Porcine vein endothelial cells treated by alpha-Galactosidase were used to investigate the expression of Gal alpha(1,3) Gal on porcine vascular endothelial cells, and the morphology of vascular endothelial cells. The results showed that Gal alpha (1,3) Gal (alpha-Gal) on porcine vascular endothelial cells, which is the major epitome of xenoantigen, could be digested by alpha-Galactosidase within 30 minutes. No morphological changes were observed in the porcine vascular endothelial cells within a period of one hour after the treatment. This suggested that the treatment of porcine tissues with alpha-Galactosidase could be a new and effective way to prevent hyperacute rejection after xenotransplantation.
Wen
J
J
Department of General Surgery, First Affiliated Hospital, WCUMS, Chengdu 610041.
Luo
Y
Y
Shen
W
W
D000818:Animals; D004187:Disaccharides / Q000276:immunology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D000939:Epitopes / Q000378:metabolism; D005260:Female; D006084:Graft Rejection / Q000276:immunology* / Q000517:prevention & control; D008297:Male; D013552:Swine; D014183:Transplantation, Heterologous; D000519:alpha-Galactosidase / Q000494:pharmacology*
D004187:Disaccharides; D000939:Epitopes; C055075:galactosyl-(1-3)galactose; D000519:alpha-Galactosidase