title: Affinity Purification of Hepatitis B Virus Surface Antigen Containing PreS1 Region.
abstract: The large protein of hepatitis B virus surface antigen (LHBs) contained an attachment site of HBV to liver cells and the antibodies to preS1 were virus-neutralizing. Therefore, vaccines containing preS1 would be more protective. However, One of the key problems in the preparation of gene-expressed proteins was the purification of the products. We have established a method of immuno-affinity chromatography with the anti-HBsAg-preS1 monoclonal antibody. Using this technique, We have successfully purified gene-expressed fusion protein of hepatitis B virus surface antigen containing preS1 (21-47). Results showed that this method was simple, effective and specific as compared to other methods, and might be useful in the future.
authors.affiliation: Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
authors.forename: Hai-Lin
authors.initials: HL
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Jin
authors.forename: Hui-Ting
authors.initials: HT
authors.lastname: Cao
authors.forename: Xie
authors.initials: X
authors.lastname: Xu
authors.forename: Guang-Di
authors.initials: GD
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Zu-Chuan
authors.initials: ZC
authors.lastname: Zhang