A family of novel DNA sequencing instruments based on single-photon detection.
We have developed a family of high-performance capillary DNA sequencing instruments based on a novel multicolor fluorescent detection technology. This technology is based on two technical innovations: the multilaser excitation of fluorescence of labeled DNA fragments and the "color-blind" single-photon detection of modulated fluorescence. Our machines employ modern digital and broadband techniques that are essential for achieving superior instrument performance. We discuss the design and testing results for several versions of the automated single lane DNA sequencers, as well as our approach to scaling up to multilane instruments.
Alaverdian
Ludmila
L
Department of Electrical and Computer Engineering, State University of New York-Stony Brook, Stony Brook, NY 11794-2350, USA.
Alaverdian
Sinok
S
Bilenko
Olga
O
Bogdanov
Iouri
I
Filippova
Elena
E
Gavrilov
Dmitry
D
Gorbovitski
Boris
B
Gouzman
Michael
M
Gudkov
Georgy
G
Domratchev
Sergey
S
Kosobokova
Olga
O
Lifshitz
Nadia
N
Luryi
Serge
S
Ruskovoloshin
Victor
V
Stepoukhovitch
Andrew
A
Tcherevishnick
Marina
M
Tyshko
Georgy
G
Gorfinkel
Vera
V
D003116:Color; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D005456:Fluorescent Dyes; D007834:Lasers; D017785:Photons; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation*
D005456:Fluorescent Dyes