title: A human sex hormone-binding globulin isoform accumulates in the acrosome during spermatogenesis.
abstract: Human sex hormone-binding globulin (SHBG) binds estradiol and testosterone with high affinity. Plasma SHBG is produced by hepatocytes, but the human SHBG gene is also expressed in the testis. Little is known about SHBG gene expression in the human testis, but human SHBG transcripts accumulate in a spermatogenic stage-dependent manner in the testes of mice containing an 11-kb human SHBG transgene. We have now found that human SHBG transcripts containing an alternative exon 1 sequence are located specifically in the testicular germ cells of these transgenic mice, whereas murine SHBG transcripts are confined to Sertoli cells. In addition, we have detected immunoreactive human SHBG in the acrosome during all stages of spermiogenesis in mice containing an 11-kb human SHBG transgene. Western blots of germ cell extracts from these transgenic mice and from human sperm indicate that the immunoreactive human SHBG in the acrosome composes electrophoretic variants, which are 3-5 kDa smaller than the major electrophoretic isoforms of human SHBG in the blood. This apparent size difference is due in part to differences in glycosylation of plasma and acrosomal SHBG isoforms. The function of the human SHBG isoform in the acrosome is unknown, but it binds steroid ligands with high affinity. This is the first demonstration that human SHBG transcripts encode an SHBG isoform that remains within a cellular compartment.
authors.affiliation: Department of Obstetrics & Gynecology, Canadian Institutes of Health Research Group in Fetal and Neonatal Health and Development, University of Western Ontario, London, Ontario N6A 4L6, Canada.
authors.forename: David M
authors.initials: DM
authors.lastname: Selva
authors.forename: Kevin N
authors.initials: KN
authors.lastname: Hogeveen
authors.forename: Koji
authors.initials: K
authors.lastname: Seguchi
authors.forename: Francis
authors.initials: F
authors.lastname: Tekpetey
authors.forename: Geoffrey L
authors.initials: GL
authors.lastname: Hammond
mesh_terms: D000177:Acrosome / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D005091:Exons / Q000235:genetics; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012738:Sex Hormone-Binding Globulin / Q000235:genetics / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism; D013737:Testis / Q000502:physiology; D019076:Transgenes
chemical_list: D020033:Protein Isoforms; D012738:Sex Hormone-Binding Globulin