Alteration in expression of polyamine and glucose-related enzyme mRNA after small bowel resection in the rat residual ileum.
The adaptive hyperplasia of the residual intestine after a massive bowel resection is not fully understood. We investigated the alterations in polyamine and glucose-related enzyme mRNA expression during intestinal adaptation. Six-week-old male Wistar rats underwent an 80% resection of the small intestine. The residual ileum was removed on the preoperative day (control) and on postoperative day (POD) 1, 3, 5 and 7. The total RNA was extracted from the mucosa, and a Northern blot analysis was performed. In the residual small intestine, the expression of polyamine synthesis enzymes, ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (SAMDC) mRNAs were increased on POD 1. The expression of polyamine degradation enzymes diamine oxidase (DAO) and spermidine/spermine N1-acetyltransferase (SSAT) mRNA did not change dramatically. Antizyme-1 (AZ-1) mRNA was significantly increased on POD 1. The mRNA expression of glucose absorption and metabolism-related proteins, including the Na+-dependent D-glucose cotransporter (SGLT1), fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase (Fru-6-P,2-kinase/Fru-2,6-Pase) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were only slightly changed on POD 1. The enzymes responsible for polyamine biosynthesis but not catabolism were upregulated at the translational level in enterocytes after a small bowel resection. The expression of glucose transport and glycolysis enzyme mRNAs did not increase after a small bowel resection.
Tsujikawa
Tomoyuki
T
Division of Gastroenterology, Shiga University of Medical Science, Tsukinowa-cho, Seta, Otsu, Shiga 520-2192, Japan. tujikawa@belle.shiga-med.ac.jp
Fukunaga
Tetsuya
T
Itoh
Akihiko
A
Satoh
Jin
J
Yasuoka
Takashi
T
Sasaki
Masaya
M
Fujiyama
Yoshihide
Y
Bamba
Tadao
T
D012437:Adenosylmethionine Decarboxylase / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D005947:Glucose / Q000378:metabolism*; D007421:Intestine, Small / Q000201:enzymology* / Q000601:surgery*; D008297:Male; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000235:genetics; D011073:Polyamines / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
D011073:Polyamines; D012333:RNA, Messenger; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase; D005947:Glucose