Specific immunological detection of the (V+C)(-) fibronectin isoform.
The population of fibronectins in adult mammalian cartilage includes high levels of a cartilage-specific (V+C)(-) isoform which lacks the V, III-15, and I-10 segments and thus contains a novel junction between protein segments III-14 and I-11. We report production of a monoclonal antibody specific for (V+C)(-) fibronectin without cross-recognition of V(+)C(+) and V(-)C(+) isoforms found in plasma and other tissues. Presentation of epitope to this antibody requires the III-14/I-11 junction, but the epitope itself extends beyond 14 amino acids immediately surrounding the junction site and involves a conformational change in III-14 and/or the N-terminal portion of I-11. The antibody, designated Mab 5D10 anti (V+C)(-), displays specificity for (V+C)(-) fibronectin from multiple mammalian species including humans and utility in immunoblots, immunohistochemistry, and ELISA.
Burton-Wurster
Nancy
N
James A Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. niwl@cornell.edu
Chen
Hao
H
Gendelman
Rina
R
Jackson
Michael L
ML
Gagliardo
Lucille F
LF
Gu
Da Nian
DN
Zelko
Russell R
RR
Lust
George
G
MacLeod
James N
JN
D000818:Animals; D000911:Antibodies, Monoclonal; D002356:Cartilage / Q000737:chemistry; D003429:Cross Reactions; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007158:Immunologic Techniques; D020033:Protein Isoforms / Q000032:analysis; D013582:Synovial Fluid / Q000737:chemistry
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D020033:Protein Isoforms