title: Expression and Activity of Recombinant Human Augmenter of Liver Regeneration.
abstract: The cDNA of human augmenter of liver regeneration was subcloned onto the downstream of the P(R)P(L) promoter of the expression plasmid pBV220. The recombinant plasmid could stably express ALR with high efficiency (up to 20% of the total bacterial proteins) in E. coli through thermal induction. The expressed recombinant ALRs could produce a significant increase in the incorporation of (3)H-TdR into liver DNA of a 1/3 hepatectomized test animal and also had a potent antihepatitis effect. These results suggest that ALR appears to be an important regulator of liver regeneration and may be used in clinical trial for enhancing liver regeneration in the treatment of hepatic diseases.
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Xie
authors.forename: Hao
authors.initials: H
authors.lastname: He
authors.forename: Han-Dong
authors.initials: HD
authors.lastname: Wei
authors.forename: Zu-Ze
authors.initials: ZZ
authors.lastname: Wu
authors.forename: Fu-Chu
authors.initials: FC
authors.lastname: He