Effect of short-term hypothermia on lipid peroxidation and antioxidant enzyme activity in rats.
This experiment was carried out to determine the effect of short-term hypothermia on blood malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glucose-6-phosphate dehydrogenase (G-6-PD) concentrations in rats. Twenty Sprague-Dawley rats were used weighing 180-200 g and on average 3.5 months old. They were randomly divided into two experimental groups: control (without cooling) and hypothermic (with cooling). The rats of the hypothermic group were cooled by immersion into cold water (10-12 degrees C), and the control rats were immersed into water of body temperature (37 degrees C) up to the neck without using any anaesthetic or tranquilizer for 3 min Rectal body temperatures of both groups were measured and blood samples to analyse MDA, GSH, SOD, GSH, GSH-Px and G-6-PD were collected immediately after the treatment. It was found that the MDA level was higher and the GSH and G-6-PD levels were lower in the hypothermic group than those in the controls. There was no difference between the control or hypothermic group regarding SOD or GSH-Px levels. It is concluded that acute hypothermia increased the lipid peroxidation and decreased the GSH and G-6-PD levels in rats.
Dede
S
S
Yuzuncu Yil Universitesi, Veteriner Fakultesi, Biyokimya Anabilim Dali, Van, Turkey. ssdede@hotmail.com
Deger
Y
Y
Meral
I
I
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001831:Body Temperature; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D007035:Hypothermia / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood; D051381:Rats / Q000097:blood / Q000378:metabolism*; D017207:Rats, Sprague-Dawley / Q000097:blood / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood
D000975:Antioxidants; D008315:Malondialdehyde; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione