Identification of cultured progenitor cells from human marrow stroma.
The marrow stromal cells (MSC) are essential for regulation of bone remodeling and hematopoiesis. It is of prime importance to isolate MSC and to expand the proliferating cells ex vivo. In this study, we analyzed cultured MSC for various cellular parameters, including cell morphology, cell cycle, and expression of cell surface antigens by flow cytometry. MSC were divided based on cell size to small (S-cells) and large (L-cells) and were visualized by light and electron microscope. The S-cells were proliferating cells correlated with G0/G1 phase of cell cycle, and expressed cFOS. The expression of surface markers CD-34, -44, -51, -61, -62E, -62P, -62L was quantified using flow cytometry. CD-44 was ubiquitously expressed by S and L cells, CD-51 and -61 were expressed by 30%-38% of S-cells. CD-34 and -62 expressed 20% positive of the analyzed cells that were of the proliferating progenitors (S-cells). This study enables the identification of subpopulations from MSC with special attention paid to the proliferating cells from ex vivo cultures of marrow stroma.
Shur
I
I
Department of Cell Biology and Histology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv 66978, Israel.
Marom
R
R
Lokiec
F
F
Socher
R
R
Benayahu
D
D
D018952:Antigens, CD34 / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology*; D016723:Bone Remodeling; D002455:Cell Division; D002478:Cells, Cultured; D005434:Flow Cytometry; D016193:G1 Phase; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D039564:Integrin alphaV / Q000096:biosynthesis; D039661:Integrin beta3 / Q000096:biosynthesis; D019041:L-Selectin / Q000096:biosynthesis; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D019007:P-Selectin / Q000096:biosynthesis; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016192:Resting Phase, Cell Cycle; D016196:S Phase; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*
D018952:Antigens, CD34; D018960:Hyaluronan Receptors; D039564:Integrin alphaV; D039661:Integrin beta3; D019007:P-Selectin; D016760:Proto-Oncogene Proteins c-fos; D019041:L-Selectin