Characterization and Identification of Recombinant [B18Ile] Human Insulin.
Recombinant [B18Ile] human insulin was obtained from a mutant [B18Ile] PIP purified by transpeptidation. [B18Ile] human insulin can be crystallized and has 82% of receptor binding activity as that of porcine insulin and retains almost the same level of in vivo biological activity comparing with porcine insulin. It is proposed that the B18Val residue may not be involved in the expression of insulin activity.
Liu
Bin
B
State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
Tang
Yue-Hua
YH
Liang
Zhen-He
ZH
Feng
You-Min
YM