title: [Epitope mapping of hepatitis C virus non-structure protein 5 from a 7 peptide phage library by using immobilized specific monoclonal antibody].
abstract: OBJECTIVE
  To screen HCV NS5 mimotopes by using monoclonal antibody and phage peptide library.
  
  
  METHODS
  By using HCV NS5 monoclonal antibody as selective molecule, a 7 peptide phage library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.
  
  
  RESULTS
  Twelve positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was confirmed as the mimotope of HCV NS5.
  
  
  CONCLUSIONS
  HCV mimotope is obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development.
authors.affiliation: Gene Therapy Research Center, Institute of infectious Diseases, 302th Hospital of PLA, Beijing 100039, China.
authors.forename: Yan Wei
authors.initials: YW
authors.lastname: Zhong
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Xiao Jun
authors.initials: XJ
authors.lastname: Tian
authors.forename: Xinhua
authors.initials: X
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Ling Xia
authors.initials: LX
authors.lastname: Zhang
authors.forename: Ju Mei
authors.initials: JM
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D006526:Hepatitis C / Q000628:therapy; D019151:Peptide Library; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology; D012324:RNA-Dependent RNA Polymerase
chemical_list: D000911:Antibodies, Monoclonal; D019151:Peptide Library; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase