[Determination of flavonoids in pollen typhae(Puhuang) by HPCE and HPLC].
OBJECTIVE
To determine two flavonoids isorhamnetin-3-O-neohesperidoside(I) and typheneoside(II) in Pollen Typhae by HPCE and HPLC.


METHODS
HPCE conditions: buffer = 0.02 mol.L-1 borate, 0.05 mol.L-1 SDS and 10% acetonitrile; V = 20 kV; T = 30 degrees C; lambda = 270 nm; sample size = 30 kPa.s, HPLC conditions: C18 lumn, acetonitrile-water (15:85) as mobile phase detection wavelength at 254 nm.


RESULTS
The contents of flavonoids I and II in five different Typha species and eighteen commercial samples of traditional Chinese drug Puhuang were between 0.1%-0.6%.


CONCLUSIONS
These two methods are rapid, simple, accurate and can be used for the quality control of this traditional Chinese drug.
Yang
Y
Y
Hunan Academy of Traditional Chinese Medicine and Materia Medica, Changsha 410006.
Wang
S
S
Zhang
S
S
D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D044948:Flavonols; D006027:Glycosides / Q000032:analysis*; D006569:Hesperidin / Q000031:analogs & derivatives / Q000032:analysis*; D019684:Magnoliopsida / Q000737:chemistry* / Q000145:classification; D011058:Pollen / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis*
D044948:Flavonols; D006027:Glycosides; C469309:isorhamnetin-3-O-neohesperidoside; C051428:typhaneoside; D011794:Quercetin; D006569:Hesperidin