Microbial transformation of hydrocortisone by Acremonium strictum PTCC 5282.
The ability of a genus of cephalosporium-like fungus isolated from soil, Acremonium strictum PTCC 5282, for hydrocortisone biotransformation has been investigated. This potential had not been previously examined. The fermentation yielded 11beta,17beta-dihydroxyandrost-4-en-3-one, 11beta,17alpha,20beta,21-tetrahydroxypregn-4-en-3-one and 21-acetoxy-11beta,17alpha,20-trihydroxypregn-4-en-3-one. Each microbial metabolite was purified and characterized using spectroscopic methods.
Faramarzi
Mohammad Ali
MA
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, 14174, Tehran, Iran.
Yazdi
Mojtaba Tabatabaei
MT
Shafiee
Abbas
A
Zarrini
Gholamreza
G
D000164:Acremonium / Q000378:metabolism*; D000732:Androstanols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D001711:Biotransformation; D003460:Crystallization; D005285:Fermentation; D006854:Hydrocortisone / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D017550:Spectroscopy, Fourier Transform Infrared
D000732:Androstanols; D006854:Hydrocortisone