Enzymatic hydrolysis of chemosynthesized atactic poly(3-hydroxybutyrate) by poly(3-hydroxyalkanoate) depolymerase from Acidovorax Sp. TP4 and Ralstonia pickettii T1.
The enzymatic degradability of chemosynthesized atactic poly([R,S]-3-hydroxybutyrate) [a-P(3HB)] by two types of extracellular poly(3-hydroxyalkanoate) (PHA) depolymerases purified from Ralstonia pickettii T1 (PhaZ(ral)) and Acidovorax Sp. TP4 (PhaZ(aci)), defined respectively as PHA depolymerase types I and II according to the position of the lipase box in the catalytic domain, were studied. The enzymatic degradation of a-P(3HB) by PhaZ(aci) depolymerase was confirmed from the results of weight loss and the scanning electron micrographs. The degradation products were characterized by one- and two-dimension (1)H NMR spectroscopy. It was found that a-P(3HB) could be degraded into monomer, dimer, and trimer by PhaZ(aci) depolymerase at temperatures ranging from 4 to 20 degrees C, while a-P(3HB) could hardly be hydrolyzed by PhaZ(ral) depolymerase in the same temperature range. These results suggested that the chemosynthesized a-P(3HB) could be degraded in the pure state by natural PHA depolymerase.
Wang
Yi
Y
Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Inagawa
Yasuhide
Y
Osanai
Yasushi
Y
Kasuya
Ken-ichi
K
Saito
Terumi
T
Matsumura
Shuichi
S
Doi
Yoshiharu
Y
Inoue
Yoshio
Y
D001673:Biodegradation, Environmental; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D016946:Gram-Negative Aerobic Rods and Cocci / Q000201:enzymology; D006868:Hydrolysis; D006885:Hydroxybutyrates / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000378:metabolism*; D013696:Temperature; D000094122:Polyhydroxybutyrates
D002265:Carboxylic Ester Hydrolases; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C077226:poly(3-hydroxyalkanoic acid) depolymerase