DNA microarray analysis of differentially expressed genes responsive to bisphenol A, an alkylphenol derivative, in an in vitro mouse Sertoli cell model.
To identify genes elicited by bisphenol A (BPA) in Sertoli cells, we carried out a microarray analysis of TTE3 cells (a mouse Sertoli cell line) treated with BPA. BPA (100, 200 and 400 microM) induced cell death concentration-dependently, with levels being 25%, 33% and 96%, respectively. Of the 1,081 genes analyzed, 3 genes showed decreased levels of expression while the remaining 10 genes showed increased levels in the cells treated with a subtoxic dose of BPA (200 microM). The expressions of six genes were confirmed by the TaqMan assay. These findings suggest that DNA microarray analysis is a useful tool for investigating the molecular mechanisms of the toxic effects of BPA in testicular cells.
Tabuchi
Yoshiaki
Y
Molecular Genetics Research Center, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Japan. ytabu@ms.toyama-mpu.ac.jp
Zhao
Qing-Li
QL
Kondo
Takashi
T
D000818:Animals; D001559:Benzhydryl Compounds; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D019800:Phenol / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology*; D012707:Sertoli Cell Tumor / Q000378:metabolism*; D013736:Testicular Neoplasms / Q000378:metabolism*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D019800:Phenol; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds