title: Capillary electrophoretic separation by double-strand polyaniline-coate capillaries of the advanced glycation endproducts formed from N-alpha-acetyl-L-lysine with reducing sugars.
abstract: Capillary electrophoresis using a capillary coated with a double-strand coating of polyaniline:poly(methyacrylate-co-acrylic acid) (PAN:P[MA-AAI) was used to separate advanced glycation endproducts (AGEs) formed at 37 degrees C from model systems containing either glucose (Glc), fructose (Fru), or glyceraldehyde (GA) and N-alpha-acetyl-L-lysine (NALys). The presence of the P(MA-AA) as a second strand in the polymer allows the maintenance of the conductive state of the PAN at a wide pH range. Effects of buffer pH and coating concentration on the electroosmotic flow (EOF) were investigated. More AGE species can be detected for the GA/NALys mixtures using this coated capillary than upon an uncoated capillary. The coating procedure is simple and the stability of the coated capillary is good.
authors.affiliation: Department of Chemistry, University of Rhode Island, Kingston 02882, USA.
authors.forename: Paula F G
authors.initials: PF
authors.lastname: de Sa
authors.forename: Christina
authors.initials: C
authors.lastname: Robb
authors.forename: Elmo
authors.initials: E
authors.lastname: Resende
authors.forename: Patrick
authors.initials: P
authors.lastname: McCarthy
authors.forename: Sze C
authors.initials: SC
authors.lastname: Yang
authors.forename: Phyllis R
authors.initials: PR
authors.lastname: Brown
authors.forename: Joel A
authors.initials: JA
authors.lastname: Dain
mesh_terms: D002241:Carbohydrates; D019075:Electrophoresis, Capillary / Q000379:methods*; D017127:Glycation End Products, Advanced / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D002241:Carbohydrates; D017127:Glycation End Products, Advanced; D007202:Indicators and Reagents; C002315:N(alpha)-acetyllysine; D008239:Lysine