Chinese medicine induces neurite outgrowth in PC12 mutant cells incapable of differentiation.
During continuous culture of neural PC12 cells, we obtained a drug-hypersensitive PC12 mutant cell that showed high stimulation of neurite outgrowth by various drugs. When several Chinese medicines such as shu-jing-huo-xie-tang and Wu-Ling-San were provided to these PC12 mutant cells, the frequency of nerve growth factor (NGF)-induced neurite outgrowth increased approximately 30-fold compared to NGF alone. Neurite outgrowth induced by NGF in PC12 cells is accompanied by sustained activation of mitogen-activated protein kinase (MAPK); however, these Chinese medicines did not induce MAPK activity. The findings thus indicate that certain Chinese medicines may induce neurite outgrowth by a novel mechanism which is distinct from the NGF-activated pathway in PC12 mutant cells.
Kano
Yoshio
Y
Department of Health Science, Kibi International University, Takahashi-city, Okayama, Japan. yoshio@kiui.ac.jp
Takaguchi
Shinichiro
S
Nohno
Tsutomu
T
Hiragami
Fukumi
F
Kawamura
Kenji
K
Iwama
Michael K
MK
Miyamoto
Kanji
K
Takehara
Manabu
M
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D020935:MAP Kinase Signaling System / Q000187:drug effects; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects*; D016716:PC12 Cells / Q000187:drug effects; D008517:Phytotherapy; D010946:Plants, Medicinal; D051381:Rats; D012585:Sciatica / Q000517:prevention & control
D004365:Drugs, Chinese Herbal; D020932:Nerve Growth Factor