title: Phenotype, antigen-presenting capacity, and migration of antigen-presenting cells in young and old age.
abstract: In the present paper, we investigated whether the phenotype, the antigen-presenting capacity, and the migration of antigen-presenting cells (APCs), are affected by the aging process. APCs were obtained incubating peritoneal monocyte-macrophage cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) (immature APCs) or GM-CSF and IFNgamma (mature APCs). Phenotypically, after 8 days incubation, APCs cultures were composed of CD11c and Mac-3 cells, with a similar representation, both in young and old mice. The absolute number and the expression of MHC I and II, CD80, and CD86 both on immature and mature APCs were not significantly different in young and old mice. APCs from old mice induced similar lymphocyte proliferative responses but lower lymphocyte cytotoxicity and a reduced number of CD8(+) T cells producing IFNgamma in comparison with APCs from young animals. Lymphocyte responses were antigen-specific, since TS/A pulsed APCs induced lymphocyte cytotoxicity against TS/A but not against syngeneic TUBO tumor cells. The low expression of the mRNA for the migratory CCR7 chemokine receptor present in immature APCs from old mice was greatly increased in mature APCs up to the levels found in APCs from young animals. The in vivo migration of APCs was higher in old than in young mice. These results demonstrate that some alterations in APCs function are present in aging, suggesting that an increased migratory capacity of old APCs may be required to balance their reduced antigen presentation to cytotoxic lymphocytes.
authors.affiliation: Laboratory of Tumor Immunology, Immunology Center, Department of Gerontology Research, INRCA, IRCCS, Via Birarelli 8, 60121 Ancona, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Donnini
authors.forename: Katy
authors.initials: K
authors.lastname: Argentati
authors.forename: Romina
authors.initials: R
authors.lastname: Mancini
authors.forename: Arianna
authors.initials: A
authors.lastname: Smorlesi
authors.forename: Beatrice
authors.initials: B
authors.lastname: Bartozzi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Bernardini
authors.forename: Mauro
authors.initials: M
authors.lastname: Provinciali
mesh_terms: D000375:Aging / Q000276:immunology*; D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology*; D002465:Cell Movement; D003602:Cytotoxicity, Immunologic; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000235:genetics
chemical_list: C515371:Ccr7 protein, mouse; D054400:Receptors, CCR7; D019707:Receptors, Chemokine