Protective effect of verbascoside on 1-methyl-4-phenylpyridinium ion-induced neurotoxicity in PC12 cells.
The neuroprotective effects of verbascoside, one of phenylpropanoid glucoside isolated from the Chinese herbal medicine Buddleja officinalis Maxim, on 1-methyl-4-phenylpyridinium ion (MPP(+)) induced apoptosis and oxidative stress in PC12 neuronal cells were investigated. Treatment of PC12 cells with MPP(+) for 48 h induced apoptotic death as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry, the activation of caspase-3 measured by the caspase-3 activity assay kit, the reduction in mitochondrial membrane potential with laser scanning confocal microscopy and the increase in the extracellular hydrogen peroxide level. Simultaneous treatment with verbascoside markedly attenuated MPP(+)-induced apoptotic death, increased extracellular hydrogen peroxide level, the activation of caspase-3 and the collapse of mitochondrial membrane potential. These results strongly indicate that verbascoside may provide a useful therapeutic strategy for the treatment of oxidative stress-induced neurodegenerative disease such as Parkinson's disease.
Sheng
Guo-Qing
GQ
Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, 100083, PR China. sheng1974@163.com
Zhang
Jin-Rong
JR
Pu
Xiao-Ping
XP
Ma
Jian
J
Li
Chang-Ling
CL
D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000818:Animals; D032861:Buddleja / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology; D016716:PC12 Cells; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D059808:Polyphenols
D015655:1-Methyl-4-phenylpyridinium; D005960:Glucosides; D006861:Hydrogen Peroxide; D018696:Neuroprotective Agents; D010636:Phenols; C058956:acteoside; D059808:Polyphenols