[The influence of pineal peptides on in vitro immunoglobulin secretion by Peyer's patches in rats].
The influence of the pineal peptides (1 mcg/ml) on immunoglobulin secretion by rat's Peyer's patches isolated from nonimmunized rats and from orally immunized by ovalbumin during 40 min incubation, was investigated. Using alpha- and beta-adrenoblockers--phentolamine hydrochloride (0.02 mg/ml) and anaprilin (0.6 mg/ml), respectively--the possibility of pineal peptides' effect on the gut secondary lymphoid organ' adrenoreceptors, was evaluated. Basic levels of immunized rats' control Peyer's patches secretory activity were 2.4--fold (p < 0.01) higher than those of non-immunized rats. The effect of pineal peptides on antibody-producing cells' secretory activity depended on Peyer's patches' functional condition. Application of pineal peptides led to a 35% (p < 0.05) increasing of immunoglobulin secretion by non-immunized rat Peyer's patches and to a 25% (p < 0.05) decreasing of antibody production by immunized rats lymphoid organs. These data suggest that the pineal peptides activation influence on Peyer's patches antibody-producing cells of nonimmunized rats connected with alpha-adrenoreceptors, and inhibiting action of these peptides on immunized rats lymphoid organs immunoglobulin secretion are not mediated through adrenoreceptors.
Zimina
O A
OA
St. Petersburg State University, St. Petersburg, 199034, Universitetskaya Nab., 7/9, Russia.
Kovalenko
R I
RI
Nozdrachev
A D
AD
D018674:Adrenergic Antagonists / Q000494:pharmacology; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D005260:Female; D007114:Immunization; D007136:Immunoglobulins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D010047:Ovalbumin / Q000276:immunology; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology*; D010870:Pineal Gland / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D011941:Receptors, Adrenergic / Q000378:metabolism
D018674:Adrenergic Antagonists; D007136:Immunoglobulins; D010455:Peptides; D011941:Receptors, Adrenergic; D010047:Ovalbumin