Characterisation of a hydroxymandelate oxidase involved in the biosynthesis of two unusual amino acids occurring in the vancomycin group of antibiotics.
ORF22 from the chloroeremomycin gene cluster has been cloned, expressed and characterised as a hydroxymandelate oxidase (HmO) that is involved in the formation of both (S)-4-hydroxyphenylglycine and (S)-3,5-dihydroxyphenylglycine.
Li
T L
TL
Cambridge Centre for Molecular Recognition, Department of Chemistry, Lensfield Road, Cambridge, UK CB2 1EW.
Choroba
O W
OW
Charles
E H
EH
Sandercock
A M
AM
Williams
D H
DH
Spencer
J B
JB
D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000596:Amino Acids / Q000096:biosynthesis*; D001426:Bacterial Proteins; D002849:Chromatography, Gas; D015964:Gene Expression Regulation, Bacterial; D005998:Glycine / Q000031:analogs & derivatives* / Q000096:biosynthesis*; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015394:Molecular Structure; D010088:Oxidoreductases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012118:Resorcinols; D013053:Spectrophotometry; D014640:Vancomycin / Q000031:analogs & derivatives* / Q000737:chemistry*
D000596:Amino Acids; D001426:Bacterial Proteins; D008565:Membrane Proteins; C435514:ORF22 protein, Corynebacterium glutamicum; D012118:Resorcinols; C112142:chloroeremomycin; C079215:3,5-dihydroxyphenylglycine; D014640:Vancomycin; C023010:4-hydroxyphenylglycine; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C021538:L-mandelate dehydrogenase; D005998:Glycine