title: [Rapid analysis of glufosinate by improving the bulletin method and its application to soybean and corn].
abstract: A rapid analytical method for residues of the herbicide glufosinate [DL-homoalanin-4-yl (methyl)phosphinic acid] and its metabolite (MPPA: 3-methylphosphinicopropionic acid) in soybeans and corns was developed by improving the bulletin method. Fifty mL of solution extracted with water (corresponding to 2 g of the sample) was loaded on a column packed with 5 mL of anion exchange resin, and then the trapped glufosinate and MPPA were eluted with 40 mL of 50% acetic acid. After the derivatization of glufosinate and MPPA with trimethyl orthoacetate, the derivatives were purified and separated on a silica gel cartridge column. The determination of the derivatives was performed with a GC-FPD. The detection limits for glufosinate and MPPA were 0.01 microgram/g and 0.005 microgram/g, respectively. The recoveries of glufosinate from soybeans and corns were 86.3-92.0%, and those of MPPA were 86.5-95.2%.
authors.affiliation: Kanagawa Prefectural Public Health Laboratory: 1-1-1, Nakao, Asahi-ku, Yokohama 241-0815, Japan.
authors.forename: Sadao
authors.initials: S
authors.lastname: Watanabe
mesh_terms: D000613:Aminobutyrates / Q000032:analysis*; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D006540:Herbicides / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000613:Aminobutyrates; D005227:Fatty Acids; D006540:Herbicides; D010573:Pesticide Residues; C061191:15-phenyl-beta-methylpentadecanoic acid; C003121:phosphinothricin