In situ tetramer staining.
The development of MHC tetramer staining has opened the doors to multiple avenues of new research [Science 274 (1996) 94]. In this review, we will discuss the development and application of in situ MHC tetramer (IST) staining. We describe two independently developed IST staining methodologies and discuss current uses, limitations, future uses and the interesting biology revealed by the use of IST staining.
Skinner
Pamela J
PJ
Department of Microbiology and Great Lakes Center for AIDS Research, University of Minnesota Medical School, MMC 196, 420 Delaware St. SE, Minneapolis 55455, USA.
Haase
Ashley T
AT
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D006678:HIV / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008822:Mice, Transgenic; D020836:Protein Structure, Quaternary; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D013194:Staining and Labeling / Q000379:methods*
D015395:Histocompatibility Antigens Class I; D011948:Receptors, Antigen, T-Cell