title: Detection of marine birnavirus in the Japanese pearl oyster Pinctada fucata and seawater from different depths.
abstract: This study examines the seasonal changes of marine birnavirus (MABV) in seawater and the Japanese pearl oyster Pinctada fucata reared at different depths (2 and 15 m). Oysters and seawater were collected in 1998, and a 2-step PCR was carried out to detect MABV. Virus isolation was performed on the PCR-positive samples in the oyster. The detection rate of the MABV genome in the oyster was low during June, but increased after July at both 2 and 15 m depths. MABV was not isolated until after September, when isolation rates of 10 to 28.6% were recorded. The results suggest that growth of MABV in the oyster is similar at 2 and 15 m depth. In contrast, the MABV genome in seawater was present through the year at 15 m depth, but was not detected in summer at 2 m. This suggests that the virus is destroyed by UV and/or other factors at 2 m in summer, but is stable in deeper waters.
authors.affiliation: Center for Marine Environmental Studies, Ehime University, Matsuyama, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kitamura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tomaru
authors.forename: Zen'ichiro
authors.initials: Z
authors.lastname: Kawabata
authors.forename: Satoru
authors.initials: S
authors.lastname: Suzukii
mesh_terms: D000818:Animals; D019201:Aquabirnavirus / Q000235:genetics / Q000302:isolation & purification* / Q000528:radiation effects; D001483:Base Sequence; D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D016679:Genome, Viral; D007564:Japan; D008969:Molecular Sequence Data; D010125:Ostreidae / Q000254:growth & development / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012621:Seasons; D012623:Seawater / Q000821:virology*; D013696:Temperature; D014466:Ultraviolet Rays / Q000009:adverse effects