title: Assessing the Parasight-F test in northeastern Papua, Indonesia, an area of mixed Plasmodium falciparum and Plasmodium vivax transmission.
abstract: User-friendly, reliable, and inexpensive methods for diagnosing malaria are needed at the primary health care level. During a randomized treatment trial, the Parasight-F test was assessed on days 0, 3, 7, and 28 against standard light microscopy of Giemsa-stained thick blood smears for diagnosing Plasmodium falciparum parasitemia in patients with P. falciparum (n = 84) or P. vivax (n = 59) malaria. The median P. falciparum parasite count on day 0 was 2,373/microL (range = 20-74,432/microL). At the start of treatment, the Parasight-F test had a sensitivity of 95.2% (80 of 84; 95% confidence interval [CI] = 88.2-98.7), and a specificity of 94.9% (56 of 59; 95% CI = 85.8-98.9). On day 7, this test showed false-positive results in 17 (16.3%) of 104 patients (95% CI = 9.8-24.9). The Parasight-F test performed well when compared with light microscopy in detecting P. falciparum parasitemia in patients presenting with clinical malaria. However, the high false-positive rate on day 7 limits its use for patient follow-up.
authors.affiliation: US Naval Medical Research Unit No 2, Jakarta, Indonesia. Taylorw@who.ch
authors.forename: Walter R J
authors.initials: WR
authors.lastname: Taylor
authors.forename: Hendra
authors.initials: H
authors.lastname: Widjaja
authors.forename: Hasan
authors.initials: H
authors.lastname: Basri
authors.forename: David J
authors.initials: DJ
authors.lastname: Fryauff
authors.forename: Colin
authors.initials: C
authors.lastname: Ohrt
authors.lastname: Taufik
authors.forename: Emiliana
authors.initials: E
authors.lastname: Tjitra
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Hoffman
authors.forename: Samuel
authors.initials: S
authors.lastname: Baso
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Richie
mesh_terms: D004641:Emigration and Immigration; D006801:Humans; D007214:Indonesia; D007958:Leukocyte Count; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000635:transmission; D016780:Malaria, Vivax / Q000175:diagnosis* / Q000635:transmission; D008853:Microscopy / Q000379:methods; D010274:Parasitology / Q000379:methods; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic