Identification of GtgE, a novel virulence factor encoded on the Gifsy-2 bacteriophage of Salmonella enterica serovar Typhimurium.
The Gifsy-2 temperate bacteriophage of Salmonella enterica serovar Typhimurium contributes significantly to the pathogenicity of strains that carry it as a prophage. Previous studies have shown that Gifsy-2 encodes SodCI, a periplasmic Cu/Zn superoxide dismutase, and at least one additional virulence factor. Gifsy-2 encodes a Salmonella pathogenicity island 2 type III secreted effector protein. Sequence analysis of the Gifsy-2 genome also identifies several open reading frames with homology to those of known virulence genes. However, we found that null mutations in these genes did not individually have a significant effect on the ability of S. enterica serovar Typhimurium to establish a systemic infection in mice. Using deletion analysis, we have identified a gene, gtgE, which is necessary for the full virulence of S. enterica serovar Typhimurium Gifsy-2 lysogens. Together, GtgE and SodCI account for the contribution of Gifsy-2 to S. enterica serovar Typhimurium virulence in the murine model.
Ho
Theresa D
TD
Department of Microbiology. College of Medicine, University of Illinois, Urbana, Illinois 61801, USA.
Figueroa-Bossi
Nara
N
Wang
Minhua
M
Uzzau
Sergio
S
Bossi
Lionello
L
Slauch
James M
JM
D000818:Animals; D029968:Escherichia coli Proteins; D005260:Female; D005816:Genetic Complementation Test; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000503:physiopathology*; D012483:Salmonella Phages / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics / Q000472:pathogenicity* / Q000821:virology; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014774:Virulence / Q000235:genetics
D029968:Escherichia coli Proteins; D014764:Viral Proteins; D013482:Superoxide Dismutase; C116114:sodC protein, E coli