title: Phylogenetic analysis and in situ identification of "Nostocoida limicola"-like filamentous bacteria in activated sludge from industrial wastewater treatment plants.
abstract: The diversity of filamentous bacteria present in industrial wastewater treatment plants was analysed by a combination of classical and molecular-biological approaches. Many unknown filamentous bacteria were observed in about 80 screened activated sludge samples from different industries with sometimes severe bulking sludge problems. A special focus was paid to filaments which resembled "Nostocoida limicola", a filamentous bacterium which was found to be present in many WWTPs. These filamentous bacteria are hardly cultivable and only one strain was obtained and maintained in co-culture with a yeast. The 16S rRNA sequences of several other "Nostocoida limicola"-like filamentous bacteria from different sludge samples were obtained by micromanipulation and different molecular-biological methods. The sequences were phylogenetically analyzed and specific molecular probes were developed and applied. The results clearly demonstrate that "Nostocoida limicola"-like filaments from industrial WWTPs are different from all other "Nostocoida limicola" types investigated so far. Our strains are affiliated to the alpha-subclass of Proteobacteria.
authors.affiliation: Vermicon AG, München, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Snaidr
authors.forename: C
authors.initials: C
authors.lastname: Beimfohr
authors.forename: C
authors.initials: C
authors.lastname: Levantesi
authors.forename: S
authors.initials: S
authors.lastname: Rossetti
authors.forename: J
authors.initials: J
authors.lastname: van der Waarde
authors.forename: B
authors.initials: B
authors.lastname: Geurkink
authors.forename: D
authors.initials: D
authors.lastname: Elkelboom
authors.forename: M
authors.initials: M
authors.lastname: Lemaitre
authors.forename: V
authors.initials: V
authors.lastname: Tandoi
mesh_terms: D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D007220:Industrial Waste; D010802:Phylogeny; D020560:Proteobacteria / Q000302:isolation & purification / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D007220:Industrial Waste; D012722:Sewage