The effect of Chinese medicine on bone cell activities.
In this experiment, we investigate the biochemical effects of traditional Chinese medicines via an in vitro bone cell culture. Ten different Chinese medicines were used in this study. The rat osteoblast-osteoclast co-culture system was used as the experimental model. After the cells grew to 80% confluence, various tested materials were added. The mitochondria activity of the bone cells after exposure to various preparations of Chinese medicines was determined by colorimetric assay. Biochemical markers such as protein content, lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and acid phosphatase (ACP) titer were analyzed to evaluate the bone cell activity. When cultured with various Chinese medicines for 24 hours, only four of these ten Chinese medicines had potential beneficial effects on the bone cell culture; and only Drynaria fortunei (Kunze) J. Sm. had a universal beneficial effect on bone cell metabolism. The major beneficial effect of Drynaria fortunei (Kunze) J. Sm. on bone cells is probably mediated by the induction of apoptosis of the osteoclast cell population. Continued study of alterations in gene expression of bone cells caused by Chinese medicines will improve our understanding of bone cell responses to various pharmacological interventions.
Lin
Chuu-Yu
CY
School of Pharmacy, Taipei Medical University, Taiwan, ROC.
Sun
Jui-Sheng
JS
Sheu
Shiow-Yunn
SY
Lin
Feng-Huei
FH
Wang
Yng-Jiin
YJ
Chen
Li-Ting
LT
D000135:Acid Phosphatase / Q000187:drug effects; D000469:Alkaline Phosphatase / Q000187:drug effects; D000818:Animals; D002460:Cell Line / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007150:Immunohistochemistry; D007770:L-Lactate Dehydrogenase / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D008517:Phytotherapy; D029621:Polypodiaceae; D051381:Rats
D004365:Drugs, Chinese Herbal; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase