Structure of genes for Hsp30 from the white-rot fungus Coriolus versicolor and the increase of their expression by heat shock and exposure to a hazardous chemical.
We isolated and analysed two genomic DNAs that encode the heat-shock protein Hsp30 from Coriolus versicolor. The amino acid sequences substitute only three amino acid substitutions. The promoter regions contain the consensus heat-shock element, a xenobiotic-response element, a stress-response element, and a metal-response element. The levels of mRNAs for Hsp30 increased markedly after exposure of C. versicolor to pentachlorophenol and levels were higher than those after heat shock.
Iimura
Yosuke
Y
Institute for Environmmental Management Technology, National Institute of Advanced Industrial Science, Tsukuba, Ibaraki, Japan. y.iimura@aist.go.jp
Tatsumi
Kenji
K
D024506:5' Flanking Region / Q000235:genetics; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D001487:Basidiomycota / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D015152:Blotting, Northern; D005800:Genes, Fungal / Q000235:genetics; D050890:HSP30 Heat-Shock Proteins; D015386:Hazardous Substances / Q000633:toxicity*; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010416:Pentachlorophenol / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D012331:RNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D014475:Uncoupling Agents / Q000494:pharmacology
D050890:HSP30 Heat-Shock Proteins; D015386:Hazardous Substances; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D014475:Uncoupling Agents; D010416:Pentachlorophenol