[The mechanism of macrophage apoptosis induced by lipopolysaccharide in mice].
Apoptosis is a major form of cell death, characterized morphologically by chromatin condensation and biochemically by endonuclease cleavage of DNA into oligonucleosomal fragments. We investigated apoptosis mechanism of peritoneal macrophage(M phi) induced by peritoneal injection of lipopolysaccharide(LPS) in mice. The results showed that: LPS induced the apoptosis of peritoneal M phi and concomitant decrease of phagocytosis(vs control group, P < 0.01), the concentration of NO2-/No3- in the peritoneal lavage fluid significantly increased after LPS injection; AG(inhibitor of iNOS) and PDTC(inhibitor of reactive oxygen species) prevented the apoptosis of M phi and reduced the concentration of NO2-/NO3- in the peritoneal lavage fluid. In vitro experiment, we found that AG and PDTC inhibited the apoptosis of M phi induced by IFN(100 U.ml-1) + LPS (10 micrograms.ml-1) by using DNA gel electrophoresis analysis. These evidences support that NO and active oxygen species may be involved in the apoptosis process of peritoneal M phi induced by LPS in mice.
Luo
F J
FJ
Department of Pathophysiology, Hunan Medical University, Changsha 410078.
Xiao
X Z
XZ
You
J L
JL
Wang
Y R
YR
Luo
Z Y
ZY
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D007372:Interferons / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000473:pathology*; D008297:Male; D051379:Mice; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D011897:Random Allocation
D008070:Lipopolysaccharides; D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide; D000255:Adenosine Triphosphate; D007372:Interferons