title: [Rapid induction of mRNAs for liver regeneration genes by hepatopoietin and partial hepatectomy].
abstract: OBJECTIVE
  To investigate the effect of recombinant human hepatopoietin (rhHPO) and partial hepatectomy on rapidly induced expression of immediate early gene.
  
  
  METHODS
  We investigated the different gene expression within 1 hour after 2/3 partial hepatectomy by representational difference analysis and in primary cultured hepatocytes system.
  
  
  RESULTS
  In the expressed sequence tag (EST) library, we identified that most of these genes were immediate early gene, and found one new gene PC3 that might be associated to liver regeneration in the EST library. Moreover, PC3 gene was rapidly induced after 2/3 partial hepatectomy and the expressing peak was within 1~2 hours after operation. HPO can rapidly induce the expression of these genes (c-fos, LRF-1, and PC3, etc.) in primarily cultured rat hepatocyte, which might be one of HPO molecular mechanism on stimulating hepatocyte proliferation.
  
  
  CONCLUSIONS
  rhHPO and partial hepatectomy can rapidly induce the expression of immediate early gene. PC3 gene is immediate early gene related to liver regeneration.
authors.affiliation: Department of Digestive Diseases, Daping Hospital of Third Military Medical University, Chongqing 400042, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Wang
authors.forename: Xiao Rong
authors.initials: XR
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Hu
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: En Ren
authors.initials: ER
authors.lastname: Leng
authors.forename: Dian Chun
authors.initials: DC
authors.lastname: Fang
authors.forename: Xiao Ming
authors.initials: XM
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fu Chu
authors.initials: FC
authors.lastname: He
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D015152:Blotting, Northern; D005786:Gene Expression Regulation / Q000187:drug effects; D017781:Genes, Immediate-Early; D006498:Hepatectomy; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D008115:Liver Regeneration / Q000235:genetics*; D043484:Proprotein Convertases; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor; D043484:Proprotein Convertases; D016282:Aspartic Acid Endopeptidases