[Cloning of the recombinant human hepatocyte growth factor gene].
The full length of HGF cDNA gene(2,184 bp) was amplified successfully from human placental tissue using RT-PCR technique, and then cloned into pGEM-T vector, which was identified and confirmed by restriction endonuclease mapping using Nde I, Bg1 II, Hind III, BamH I and Xho I, as well as DNA sequencing. The above clone of HGF cDNA gene was successfully subcloned into the retrovirus vector(pLNL-XHC), which may be used for further studies of gene expression and gene therapy.
Luo
J X
JX
Department of Clinical Biochemistry, Research Center of Molecular Biology, Hunan Medical University, Changsha 410078.
Tan
W B
WB
Tan
Y N
YN
Chen
G J
GJ
D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D017228:Hepatocyte Growth Factor / Q000235:genetics*; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004274:DNA, Recombinant; D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor; D004247:DNA