Standardized staining methods: Feulgen-Rossenbeck reaction for desoxyribonucleic acid and periodic acid-Schiff (PAS) procedure.
A project group working under the European Confederation of Laboratory Medicine (ECLM) presents recommendations for standardized procedures for the Feulgen-Rossenbeck-Schiff and the periodic acid-Schiff (PAS) reactions on cytological and histological material. The advantages and disadvantages of such standardized procedures are presented here in a preamble. Both users and manufacturers are encouraged to give their opinions with a view to achieving consensus on these procedures and on how further work on these lines may proceed.
Lyon
H O
HO
Department of Pathology, Hvidovre Hospital, University of Copenhagen, Denmark. lyon@post4.tele.dk
Schulte
E K
EK
Prento
P
P
Barer
M R
MR
Béné
M-C
MC
D004396:Coloring Agents; D004247:DNA / Q000032:analysis*; D010503:Periodic Acid-Schiff Reaction / Q000592:standards; D012394:Rosaniline Dyes / Q000378:metabolism; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D014052:Toluidines / Q000378:metabolism
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D014052:Toluidines; C005409:pararosaniline; D004247:DNA