Capillary electrophoresis of Cr(VI) reducer Arthrobacter oxydans.
Rapid and effective separation of bacteria Arthrobacter oxydans was performed using capillary electrophoresis. For optimal separation of bacteria the influence of buffer concentration, pH and applied voltage were studied. It was found that the most appropriate conditions for electrophoretic mobility measurements are as follows: applied voltage 6-14 kV; buffer concentration 5-10 mM pH 6-8. At the stationary phase of growth there are always two main heterogeneous peaks. They are connected with the morphology of bacteria as well as with cell aggregation. The heterogeneity of samples may be explained by surface modifications of bacterial cells.
Tsibakhashvili
Nelly Ya
NY
E. Andronikashvili Institute of Physics, Georgian Academy of Sciences, Tamarashvili str.6, Tbilisi, Georgia. ciban2@iph.hepi.edu.ge
Asatiani
Nino V
NV
Abuladze
Marina K
MK
Birkaya
Barbara G
BG
Sapojnikova
Nelly A
NA
Mosulishvili
Liguri M
LM
Holman
Hoi-Ying N
HY
D001173:Arthrobacter / Q000302:isolation & purification* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002857:Chromium / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration
D002857:Chromium