title: Blue myoglobin reconstituted with an iron porphycene shows extremely high oxygen affinity.
abstract: Myoglobin will be a good scaffold for engineering a function into proteins. To modulate the physiological function of myoglobin, almost all approaches have been demonstrated by site-directed mutagenesis, however, there are few studies which show a significant improvement in the function. In contrast, we focused on the replacement of heme in the protein with an artificial prosthetic group. Recently, we prepared a novel myoglobin reconstituted with an iron porphycene as a structural isomer of mesoheme. The bluish colored reconstituted myoglobin is relatively stable and the deoxymyoglobin reversibly binds ligands. Interestingly, the O2 affinity of the reconstituted myoglobin, 1.1 x 109 M-1, is a significant 1,400-fold higher than that of the native myoglobin. Furthermore, the unfavorable autoxidation kinetics show 7-fold decrease in rate for the reconstituted myoglobin relative to the native myoglobin, indicating the stable oxy-form against autoxidation. The net results come from the slow dissociation of the O2 ligand in the reconstituted myoglobin, koff = 0.11 s-1, because of the formation of strong hydrogen bond between His64 and negatively charged dioxygen. The present study indicates that the replacement of native heme with an artificially created prosthetic group will give us a unique function into a hemoprotein.
authors.affiliation: Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, and PRESTO in Japan Science and Technology Corporation, Fukuoka 812-8581, Japan. thayatcm@mbox.nc.kyushu-u.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Dejima
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuo
authors.forename: Hideaki
authors.initials: H
authors.lastname: Sato
authors.forename: Dai
authors.initials: D
authors.lastname: Murata
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hisaeda
mesh_terms: D006418:Heme / Q000737:chemistry*; D007700:Kinetics; D009211:Myoglobin / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry* / Q000378:metabolism; D011166:Porphyrins / Q000737:chemistry*
chemical_list: D009211:Myoglobin; D011166:Porphyrins; C051407:porphycene; D006418:Heme; D010100:Oxygen