5-Alpha- and 5-beta-2-deoxyintegristerone A, a 5-alpha and 5-beta isomer pair of ecdysteroids isolated from the Silene genus.
5-Alpha-2-deoxyintegristerone A and 5-beta-2-deoxyintegristerone A were isolated from the aerial parts of Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman using a specific combination of absorption column chromatography, preparative thin-layer chromatography and preparative HPLC. Both normal-phase and reversed-phase modes of HPLC were employed for isolation. Structural elucidation of 5-alpha-2-deoxyintegristerone A was completed by X-ray diffraction. Both 5-alpha-2-deoxyintegristerone A and 5-beta-2-deoxyintegristerone A were firstly isolated from this plant. We propose that 5-alpha-2-deoxyintegristerone A is not an artifact but an integral part of the ecdysteroid spectrum of Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman.
Báthori
M
M
Department of Pharmacognosy, University of Szeged, 6701 Szeged, Eötvös utca 6, PO Box 121, Hungary.
Kalász
H
H
Pongrácz
Z
Z
Máthé
I
I
Kálmán
A
A
Argay
G
G
D029748:Caryophyllaceae / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D007536:Isomerism; D008958:Models, Molecular; D015394:Molecular Structure; D010840:Phytosterols / Q000737:chemistry / Q000302:isolation & purification*; D014961:X-Ray Diffraction
C471561:2-deoxyintegristerone A; D010840:Phytosterols