Effect of piperine, the active ingredient of black pepper, on intestinal secretion in mice.
We have investigated the effect piperine on castor oil-stimulated fluid accumulation in the mouse small intestine. Piperine (2.5-20 mg/kg, i.p.) dose-dependently reduced castor oil-induced intestinal fluid accumulation. The inhibitory effect of piperine (10 mg/kg i.p.) was strongly attenuated in capsaicin (75 mg/kg in total, s.c.)-treated mice but it was not modified by the vanilloid receptor antagonist capsazepine (30 mg/kg i.p.). Pretreatment of mice with hexamethonium (1 mg/kg i.p.), naloxone (2 mg/kg i.p.), yohimbine (1 mg/kg i.p.) or the cannabinoid CB(1) receptor antagonist SR141716A (0.3 mg/kg i.p.) did not modify the inhibitory effect of piperine (10 mg/kg i.p.). These results suggest that piperine reduces castor oil-induced fluid secretion with a mechanism involving capsaicin-sensitive neurons, but not capsazepine-sensitive vanilloid receptors.
Capasso
Raffaele
R
Department of Pharmaceutical Science, University of Salerno, Italy.
Izzo
Angelo A
AA
Borrelli
Francesca
F
Russo
Alessandra
A
Sautebin
Lidia
L
Pinto
Aldo
A
Capasso
Francesco
F
Mascolo
Nicola
N
D000470:Alkaloids; D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000494:pharmacology*; D052117:Benzodioxoles; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002368:Castor Oil / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D007274:Injections, Intraperitoneal; D007419:Intestinal Secretions / Q000187:drug effects*; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size / Q000187:drug effects; D010880:Piperidines / Q000008:administration & dosage / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides
D000470:Alkaloids; D000897:Anti-Ulcer Agents; D052117:Benzodioxoles; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D002368:Castor Oil; C071423:capsazepine; D002211:Capsaicin; C008922:piperine