Effects of alumina and zirconium dioxide particles on arachidonic acid metabolism and proinflammatory interleukin production in osteoarthritis and rheumatoid synovial cells.
We describe a model which can be used for in vitro biocompatibility assays of biomaterials. We studied the in vitro response of human osteoarthritis or rheumatoid arthritis fibroblast-like synoviocytes to Al2O3 or ZrO2 particles by analysing the production of interleukin-1 (IL-1) and interleukin-6 (IL-6) and the metabolism of arachidonic acid via lipoxygenase and cyclo-oxygenase pathways. Our results show that, in these cells and under our experimental conditions, Al2O3 and ZrO2 did not significantly modify the synthesis of IL-1 and IL-6 or the metabolism of arachidonic acid.
Liagre
B
B
Moalic
S
S
Vergne
P
P
Charissoux
J L
JL
Bernache-Assollant
D
D
Beneytout
J L
JL
D000537:Aluminum Oxide / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism*; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001672:Biocompatible Materials / Q000494:pharmacology; D001697:Biomedical and Dental Materials / Q000494:pharmacology*; D018929:Cell Culture Techniques; D003239:Connective Tissue Cells / Q000187:drug effects* / Q000502:physiology*; D015232:Dinoprostone / Q000096:biosynthesis; D015777:Eicosanoids / Q000096:biosynthesis; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007375:Interleukin-1 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D008422:Materials Testing / Q000379:methods*; D008954:Models, Biological; D010003:Osteoarthritis / Q000276:immunology; D015040:Zirconium / Q000494:pharmacology*
D001672:Biocompatible Materials; D001697:Biomedical and Dental Materials; D015777:Eicosanoids; D007375:Interleukin-1; D015850:Interleukin-6; D016718:Arachidonic Acid; D015040:Zirconium; D015232:Dinoprostone; D000537:Aluminum Oxide; C028541:zirconium oxide