Chromosomal amplification of the Escherichia coli lipB region confers high-level resistance to selenolipoic acid.
One of the mutants (slr7 mutant) of a wild-type Escherichia coli strain resistant to selenolipoic acid reported previously (K. E. Reed, T. W. Morris, and J. E. Cronan, Jr., Proc. Natl. Acad. Sci. USA 91:3720-3724, 1994) unexpectedly grew on minimal medium following transductional introduction of a lipA null mutation. We report that the slr7 strain carries a duplication of the lip chromosomal region that causes the phenotype of the mutant strain.
Jordan
Sean W
SW
Departments of Microbiology. Biochemistry, University of Illinois, Urbana, Illinois 61801, USA.
Cronan
John E
JE
D000217:Acyltransferases; D000975:Antioxidants / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015139:Blotting, Southern; D002876:Chromosomes, Bacterial; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins; D009154:Mutation; D010957:Plasmids; D011506:Proteins / Q000235:genetics*; D011995:Recombination, Genetic; D012643:Selenium / Q000737:chemistry / Q000494:pharmacology*; D008063:Thioctic Acid / Q000494:pharmacology*; D014161:Transduction, Genetic
D000975:Antioxidants; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C079999:LipA protein, Bacteria; D011506:Proteins; D008063:Thioctic Acid; D000217:Acyltransferases; C421483:LipB protein, E coli; D012643:Selenium