On-probe digestion of bacterial proteins for MALDI-MS.
On-probe digestion combined with MALDI mass spectrometry is studied as a rapid method for the analysis and identification of bacterial proteins. The use of trypsin adsorbed to the probe surface reduces the digestion time from hours to minutes. A high amount of trypsin must be applied to the probe for the successful digestion of bacterial proteins. Mass spectra of the digest contain a number of low-mass digest fragments. Several components of a B. subtilis bacterial digest can be identified through postsource decay and database searching.
Harris
William A
WA
Department of Chemistry, Indiana University, Bloomington 47405, USA.
Reilly
James P
JP
D001412:Bacillus subtilis / Q000737:chemistry; D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis; D010449:Peptide Mapping / Q000295:instrumentation* / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D010446:Peptide Fragments; D014357:Trypsin