title: Secretion and dynamics of herpes simplex virus in tears and saliva of patients with Bell's palsy.
abstract: OBJECTIVE
  For clarification of the direct relationship between the reactivation of herpes simplex virus and the development of Bell's Palsy, a detection of the virus genome by deoxyribonucleic acid diagnostics and a quantitative analysis of its time-course change are both needed. The authors detected the HSV genome in specimens from patients with Bell's Palsy, quantified its number of copies, and examined time-course changes.
  
  
  METHODS
  The subjects were 16 patients with Bell's Palsy. The tear fluid and saliva from the submandibular gland and the parotid gland were separately collected from the affected and unaffected sides twice or more. A total of 244 specimens were subjected to extraction of deoxyribonucleic acid, polymerase chain reaction, and microplate hybridization.
  
  
  RESULTS
  Herpes simplex virus-1 deoxyribonucleic acid was detected in 38 specimens (11.8%) from 5 patients (31%). The high detection (28.5%) was obtained within 2 weeks after onset. Detection at 3 weeks and later (2.8%) was significantly lower ( < 0.05). In three cases, deoxyribonucleic acid was also found on the unaffected side in the initial phase of the disease, but detection on that side (18.9%) was significantly lower than on the affected side (83.8%) ( < 0.01). The number of copies of the herpes simplex virus-1 genome was large on the affected side and early after the onset of the disease.
  
  
  CONCLUSIONS
  The reactivation of herpes simplex virus-1 on the affected side is involved as a pathogenic factor of Bell's Palsy. A reactivation of herpes simplex virus-1 may be generated even on the unaffected side in the early phase of the disease. Herpes simplex virus deoxyribonucleic acid was not detected in any of the examined specimens collected from the remaining 11 cases. The need for constant study to clarify other causative factors of Bell's Palsy remains.
authors.affiliation: Department of Otolaryngology, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Abiko
authors.forename: Minoru
authors.initials: M
authors.lastname: Ikeda
authors.forename: Ryo
authors.initials: R
authors.lastname: Hondo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D020330:Bell Palsy / Q000821:virology*; D005260:Female; D006561:Herpes Simplex / Q000150:complications*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000821:virology*; D018139:Simplexvirus / Q000302:isolation & purification*; D013666:Tears / Q000821:virology*