Isolation of a galactomannan that enhances macrophage activation from the edible fungus Morchella esculenta.
The edible mushroom Morchella esculenta is among the most highly prized and morphologically recognizable fungi in the world. We describe the isolation from a polar extract of M. esculenta carpophores of a high-molecular-weight galactomannan, about 1.0 million Da, that exhibits immunostimulatory activity. At 3.0 microg/mL the galactomannan polysaccharide increased NF-kappa B directed luciferase expression in THP-1 human monocytic cells to levels 50% of those achieved by maximal activating concentration (10 microg/mL) of lipopolysaccharide. This galactomannan comprises about 2.0% of the dry fungal material weight, and its glycosyl components include mannose (62.9%) and galactose (20.0%).
Duncan
Christine J G
CJ
Department of Pharmacognosy and National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, 38677, USA.
Pugh
Nirmal
N
Pasco
David S
DS
Ross
Samir A
SA
D000363:Agaricales / Q000737:chemistry*; D002460:Cell Line; D005690:Galactose / Q000032:analysis / Q000031:analogs & derivatives; D015870:Gene Expression; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics; D008262:Macrophage Activation / Q000187:drug effects*; D008351:Mannans / Q000302:isolation & purification* / Q000494:pharmacology; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D009000:Monocytes / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism
D008070:Lipopolysaccharides; D008351:Mannans; D016328:NF-kappa B; C012990:galactomannan; D008156:Luciferases; D008358:Mannose; D005690:Galactose