title: Overexpression of heat shock protein 60/10 in myocardium of patients with chronic atrial fibrillation.
abstract: BACKGROUND
  Cardiomyocytes respond to chronic atrial fibrillation with increased expression of heat shock protein 60 (HSP60). The aim of this study was to investigate whether expression of the coprotein HSP10 is also increased.
  
  
  METHODS
  Right atrial samples from 16 patients undergoing elective cardiac operation were excised and immediately frozen in liquid nitrogen. Eight patients had chronic atrial fibrillation and 8 patients were in sinus rhythm. The HSP60 and HSP10 protein levels were determined by SDS-PAGE, Western blot, and quantified by optical densitometry according to the immunoreactive bands of actin.
  
  
  RESULTS
  In myocardial samples from patients with chronic atrial fibrillation we found simultaneous upregulation of both stress proteins. HSP60 expression was more than 2.3-fold and HSP10 expression was more than 2.4-fold increased in atrial myocardium of patients with chronic atrial fibrillation.
  
  
  CONCLUSIONS
  These results indicate functional upregulation of mitochondrial HSP60 and HSP10 in response to chronic atrial fibrillation.
authors.affiliation: Department of Cardiac Surgery and Cardiovascular Research Center, University of Ulm, Germany. aschaefler@gmx.de
authors.forename: Alfons E
authors.initials: AE
authors.lastname: Schäfler
authors.forename: Kiriakos
authors.initials: K
authors.lastname: Kirmanoglou
authors.forename: Peter
authors.initials: P
authors.lastname: Pecher
authors.forename: Andreas
authors.initials: A
authors.lastname: Hannekum
authors.forename: Bernd
authors.initials: B
authors.lastname: Schumacher
mesh_terms: D000368:Aged; D001281:Atrial Fibrillation / Q000473:pathology*; D015153:Blotting, Western; D018835:Chaperonin 10 / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism*; D002908:Chronic Disease; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006325:Heart Atria / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008929:Mitochondria, Heart / Q000473:pathology; D009206:Myocardium / Q000473:pathology*; D012016:Reference Values; D015854:Up-Regulation / Q000502:physiology
chemical_list: D018835:Chaperonin 10; D018834:Chaperonin 60