Nucleotide Sequence Analysis of HaSNPV Protein Kinase.
The protein kinase gene of Helicoverpa armigera single nucleocapsid nuclear polyhedrosis virus (HaSNPV) has been cloned and sequenced. It is located approximately 1.25 kb downstream of the polyhedrin gene. The predicted molecular mass of this 267 amino acid protein (HavPK) is 31 kD. HavPK shows a 43.0% amino acid homology to vPK from Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) and a 39.0% homology to PK-1 from Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV). Comparison of HavPK with protein kinases from other organisms showed that HavPK was also homologous to the catalytic domains of eukaryotic protein kinases and strongly indicated that HavPK was a serine/threonine protein kinase.
Zhang
Chuan-Xi
CX
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
Wang
Gen
G
Hu
Cui
C
Wu
Xiang-Fu
XF