[13C]aminopyrine and [13C]caffeine breath test: influence of gender, cigarette smoking and oral contraceptives intake.
The [13C]aminopyrine breath test ([13C]ABT) measures the global activity of cytochrome P450 in vivo and is a sensitive indicator of liver metabolic dysfunction. The present study aims to determine whether gender and cigarette smoking influence the results of [13C]ABT as well as to confirm the effect of oral contraceptive steroids (OCS) intake on this metabolic test. Hundred and ten healthy subjects, including men and women, smoker and non-smoker, women taking OCS or not, were phenotyped for CYP1A2 using the [13C]caffeine breath test and underwent a [13C]ABT. Both tests showed large inter-individual variations in accordance with that of CYP450 liver content. [13C]ABT was sensitive enough to point out a significant induction or inhibition related to cigarette smoking habits or OCS. The combined effect of smoking and OCS resulted in an overall unchanged metabolic activity. Consequently, the impact of the studied conditions on the [13C]ABT parameters must be considered by clinicians or clinical investigators.
Caubet
M S
MS
Université de Montreal, Faculté de Pharmacie, Quebec, Canada. mscaubet@justine.umontreal.ca
Laplante
A
A
Caillé
J
J
Brazier
J L
JL
D000632:Aminopyrine / Q000032:analysis*; D002110:Caffeine / Q000032:analysis*; D002247:Carbon Isotopes / Q000032:analysis*; D003276:Contraceptives, Oral / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008297:Male; D012016:Reference Values; D012737:Sex Factors; D012907:Smoking; D014026:Nicotiana
D002247:Carbon Isotopes; D003276:Contraceptives, Oral; D000632:Aminopyrine; D002110:Caffeine