[The effect of FHIT gene on renal cell carcinomas].
OBJECTIVE
To study the potential role of FHIT gene in renal cell carcinomas.


METHODS
Cancerous tissues derived from 23 patients with renal cell carcinomas and an established cell line were examined for the alteration of FHIT gene transcripts and structure of genomic DNA by using RT-PCR and Southern blot hybridization assays.


RESULTS
Eight point three percent(2/24) of cancerous tissues exhibited aberrant transcripts. The transcript in the renal-cell cancer cell line appeared a small-size one when cDNA was amplified with outside nest primer; absent expression of transcript was shown when amplified with inside nest primer. About twenty percent(5/24) cancerous tissues showed genomic rearrangement in FHIT gene locus.


CONCLUSIONS
FHIT gene exhibited aberrant transcripts and genomics in some of renal cell cancers. Therefore change of FHIT gene may play a role in development of renal cell carcinomas.
Chen
X
X
Department of Urology, Xiangya Hospital, Hunan Medical University, Changsha 41008.
Shen
P F
PF
Chen
Z C
ZC
D017766:Acid Anhydride Hydrolases; D018262:Adenocarcinoma, Clear Cell / Q000235:genetics*; D017353:Gene Deletion; D015321:Gene Rearrangement; D016147:Genes, Tumor Suppressor; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase