title: Lipid peroxidation in vitamin E-deficient rats submitted to subtotal nephrectomy.
abstract: Clinical and experimental evidence has indicated that chronic renal failure (CRF) is related to increased free radical production. CRF patients show increased lipid peroxidation after a progressive reduction in vitamin E, one of the most important antioxidants. In the present study the role of vitamin E deficiency in lipid peroxidation was investigated in rats submitted to subtotal nephrectomy. Male Wistar rats were divided into groups receiving different diets for a period of 45 days: SND - sham rats submitted to a regular diet containing vitamin E; ERD nephrectomized rats submitted to a regular diet containing vitamin E; SDD - sham rats submitted to a vitamin E-deficient diet; EDD nephrectomized rats submitted to a vitamin E-deficient diet. After 30 days the Experimental animals were submitted to 5/6 nephrectomy and the Controls were submitted to sham operation. The vitamin E levels of the SDD and EDD groups were significantly reduced (p < 0.05) in plasma (4.92 +/- 1.22 and 8.37 +/- 2.09 mmol/L, respectively), liver (7.57 +/- 2.72 and 9.44 +/- 2.55mg/g tissue, respectively) and kidney (8.17 +/- 2.38 and 9.40 +/- 3.10 mg/g tissue, respectively) when compared to the SRD and ERD groups. In contrast, in the EDD group the levels of thiobarbituric acid-reactive substances, expressed as nmol/mg protein, were significantly increased (p < 0.05) in the liver (1.41 +/- 0.27) and kidney (1.67 +/- 0.47), and superoxide dismutase activity was significantly increased in the erythrocytes (4455.80 +/- 1322.63 Ug/Hb) compared to all other groups. The vitamin E-deficient diet associated with subtotal nephrectomy determined an increase in lipid peroxidation, suggesting an important role of free radicals in the development of chronic renal failure.
authors.affiliation: Faculty of Pharmacological Sciences, USP, Ribeirão Preto, Brazil.
authors.forename: Maria Bernarda C
authors.initials: MB
authors.lastname: Cordeiro
authors.forename: Terezila M
authors.initials: TM
authors.lastname: Coimbra
authors.forename: Roberto Silva
authors.initials: RS
authors.lastname: Costa
authors.forename: Mĵnica S
authors.initials: MS
authors.lastname: Meirelles
authors.forename: Alceu Afonso
authors.initials: AA
authors.lastname: Jordão
authors.forename: Helio
authors.initials: H
authors.lastname: Vannucchi
mesh_terms: D000818:Animals; D007676:Kidney Failure, Chronic / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D009392:Nephrectomy; D051381:Rats; D017208:Rats, Wistar; D014811:Vitamin E Deficiency / Q000378:metabolism*