title: Be careful with the primers when screening your clones by polymerase chain reaction.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Campus Universitario, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Alvarez
authors.forename: Víctor M
authors.initials: VM
authors.lastname: González
authors.forename: Antonio
authors.initials: A
authors.lastname: Jiménez-Ruiz
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D004251:DNA Transposable Elements; D005822:Genetic Vectors / Q000378:metabolism; D006824:Hybridization, Genetic / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004251:DNA Transposable Elements