Inducible AmpC beta-lactamase of a new member Enterobacteriaceae.
Extensive biochemical testing and 16S rRNA and rpoB sequence analysis revealed that clinical strain CF01Ent1, initially identified as Buttiauxella agrestis by the use of Api 32 biochemical strips, is a new organism in the Enterobacteriaceae family. It produced an inducible AmpC-type beta-lactamase whose sequence shares 69 to 72% identity with those of the other AmpC-type beta-lactamases of ENTEROBACTERIACEAE: This enzyme exhibits an atypical high affinity for all beta-lactams tested.
Bonnet
Richard
R
Laboratoire de Bactériologie, Faculté de Médecine, 63001 Clermont-Ferrand Cedex, France. richard.bonnet@u-clermont1.fr
Chanal
Catherine
C
Ageron
Elisabeth
E
Sirot
Danielle
D
De Champs
Christophe
C
Grimont
Patrick
P
Sirot
Jacques
J
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D004790:Enzyme Induction; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D047090:beta-Lactams / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D047090:beta-Lactams; C071526:AmpR protein, Bacteria; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; C048795:AmpC beta-lactamases; D001618:beta-Lactamases