Zymographic detection of cinnamic acid decarboxylase activity.
The manuscript includes a concise description of a new, fast and simple method for detection of cinnamic acid decarboxylase activity. The method is based on a color shift caused a by pH change and may be an excellent procedure for large screenings of samples from natural sources, as it involves no complex sample processing or purification. The method developed can be used in preliminary approaches to biotransformation processes involving detection of hydroxycinnamic acid decarboxylase activity.
Prim
Núria
N
Department of Microbiology, Faculty of Biology, University of Barcelona, Avenue Diagonal 645, 08028 Barcelona, Spain.
Pastor
F I Javier
FI
Diaz
Pilar
P
D001407:Bacillus / Q000201:enzymology*; D001431:Bacteriological Techniques; D001962:Bromcresol Purple / Q000378:metabolism; D002262:Carboxy-Lyases / Q000737:chemistry* / Q000378:metabolism*; D002934:Cinnamates / Q000378:metabolism; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration
D002934:Cinnamates; D003470:Culture Media; C029010:cinnamic acid; D001962:Bromcresol Purple; D002262:Carboxy-Lyases; C087194:phenylacrylic acid decarboxylase