Studies on properties and application of non-protected room temperature phosphorescence of propranolol.
A direct and simple non-protected room temperature phosphorimetry (NP-RTP) for determine propranolol, which using I- as a heavy atom perturber and sodium sulfite as a deoxygenator, has been developed. The phosphorescence peak wavelength maxima lambda(ex)/lambda(em) = 288/494, 522 nm. The analytical curve of propranolol gives a linear dynamic range of 8.0 x 10(-8)-2.0 x 10(-5) mol l(-1) and a detection limit of 3 x 10(-8) mol l(-1). The influence of I- concentration on RTP lifetime of propranolol was studied and the luminescence kinetic parameters were calculated. It is found that the relation between I- concentration (x) and RTP lifetime (tau) can be expressed as tau = 1.25e(-0.477x) and the rate constants of phosphorescence emission k(p) was 0.800 per ms. The method was applied directly to determination of propranolol in urine and drug tablets with a satisfactory result. The recoveries were 96.6-97.4% and the relative standard deviation was 2% for the 1.00 x 10(-6)-4.00 x 10(-6) mol l(-1) propranolol in spiked urine sample.
Long
Wen-Qing
WQ
Department of Chemistry, Tsinghua University, Beijing, People's Republic of China. lild@chem.tsinghua.edu.cn
Zhang
Zhong-Xiao
ZX
Li
Long-Di
LD
D006801:Humans; D007202:Indicators and Reagents; D049449:Luminescence; D011433:Propranolol / Q000032:analysis / Q000737:chemistry* / Q000652:urine; D013053:Spectrophotometry / Q000379:methods; D013447:Sulfites; D013696:Temperature; D013816:Thermodynamics
D007202:Indicators and Reagents; D013447:Sulfites; D011433:Propranolol; C025026:sodium sulfite