Acid phosphatase activity in excretion/secretion products from Heligmosomoides polygyrus adults: an indicator of the physiological status of the worms.
Acid phosphatase (AP) activity was detected in 24 h culture media from adult Heligmosomoides polygyrus. Female and male excretion/secretion products showed similar specific activity. For both, the AP had a pH optimum of 4.0 and was inhibited by sodium fluoride, tartaric acid, and sodium orthovanadate. The release of AP by adult worms was significantly inhibited by adverse incubation conditions (temperatures of 20 degrees C and 4 degrees C), known physiological perturbers ( t-butylhydroperoxide and sodium azide), and broad spectrum anthelmintics (albendazole, levamisole, morantel, and ivermectin). These results indicate that the AP activity level in the culture medium may be an indicator of the physiological status of the worms.
Martínez-Grueiro
M M
MM
Department of Parasitology, Faculty of Pharmacy, Complutense University, 28040 Madrid, Spain. mgrueiro@farm.ucm.es
D000135:Acid Phosphatase / Q000378:metabolism*; D015766:Albendazole / Q000494:pharmacology; D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis; D003470:Culture Media; D005260:Female; D006863:Hydrogen-Ion Concentration; D007559:Ivermectin / Q000494:pharmacology; D007978:Levamisole / Q000494:pharmacology; D008297:Male; D009015:Morantel / Q000494:pharmacology; D009351:Nematospiroides dubius / Q000201:enzymology* / Q000502:physiology*; D012680:Sensitivity and Specificity; D012969:Sodium Fluoride / Q000494:pharmacology; D013644:Tartrates / Q000494:pharmacology; D013696:Temperature; D014638:Vanadates / Q000494:pharmacology
D000947:Antigens, Helminth; D003470:Culture Media; D013644:Tartrates; D007978:Levamisole; D014638:Vanadates; D007559:Ivermectin; D009015:Morantel; D012969:Sodium Fluoride; D000135:Acid Phosphatase; D015766:Albendazole; C029768:tartaric acid