Studies on the Correlation of the TATA-box Binding Proteins of the Rat PSBP C(3) Gene with Its Transcription Activities.
The transcription of the rat C(3)(1) gene is markedly stimulated by testosterone and is confined to the ventral prostate (V.P.). The potential correlation of the TATA-box binding proteins of the C(3) gene with its highly tissue-specific and androgen-controlled expression was investigated by using electrophoresis mobility shifting assay (EMSA) and in vitro DNaseI footprinting analysis. The results showed that the nuclear proteins bound on the 40 bp C(3)(1) TATA-box DNA fragment (-50 to -10) appeared in a highly tissue-specific manner. By comparing the protein binding activities of the C(3)(1) probes with a silent allelic form C(3)(2) probe which shared sequence homology with only one bp difference in the TATA-box (A to G at -29), it was found that the loss of binding activity occurred only in the V.P. which expresses C(3) gene when A was replaced by G at -29. This finding indicated that the specific -29(A)-dependent binding proteins of the V.P. may have additional domains which facilitate the binding of androgen receptor or other cofactors involved in androgen regulation of C(3) expression.
Zhang
Yong-Lian
YL
National Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
Parker
M.G.
MG