title: An exocellular thermolysin-like metalloprotease produced by Vibrio fluvialis: purification, characterization, and gene cloning.
abstract: An exocellular metalloprotease produced by Vibrio fluvialis, an enteropathogenic vibrio, was purified and characterized. The metalloprotease (V. fluvialis protease [VFP]) was found to have very similar characteristics to V. vulnificus protease, including a molecular mass of 45kDa, sensitivity to chelating agents or competitive inhibitors for thermolysin-like metalloproteases, and the substrate specificity. The structural gene for VFP was also cloned, and its nucleotide sequence was determined. The deduced amino acid sequence confirmed that VFP was a member of the thermolysin family. VFP, like V. vulnificus protease, showed the haemagglutinating, permeability-enhancing and haemorrhagic activities in addition to the proteolytic activity toward oligopeptide, casein or elastin.
authors.affiliation: Faculty of Pharmaceutical Sciences and Okayama University, Tsushima-Naka, Okayama, 700-8530, Japan
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Miyoshi
authors.forename: Yuka
authors.initials: Y
authors.lastname: Sonoda
authors.forename: Hiroko
authors.initials: H
authors.lastname: Wakiyama
authors.forename: Md Monzur
authors.initials: MM
authors.lastname: Rahman
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Tomochika
authors.forename: Sumio
authors.initials: S
authors.lastname: Shinoda
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tobe
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008666:Metalloendopeptidases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D013820:Thermolysin / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014733:Vibrio / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010455:Peptides; D008666:Metalloendopeptidases; C466986:VfpA protein, Vibrio fluvialis; D013820:Thermolysin