Proteomic analysis of transcription factor interactions in myeloid stem cell development and leukaemia.
Recent results indicate that interactions of transcription factors with other nuclear proteins play an important role in stem cell development, lineage commitment and differentiation in the haematopoietic system, and the pathogenesis of myeloid leukaemias. High-throughput proteomics by mass spectrometric analysis of gel-separated proteins can identify multi-protein complexes and changes in the expression of multiple proteins simultaneously. This review describes an application of proteomic methods (2D gel electrophoresis (GE) and mass spectrometry (MS)), which can be used to identify regulated protein targets of transcription factors important in myeloid differentiation and leukaemia. This global high-throughput functional proteomics approach could lead to new insights into the network of protein-protein interactions and target proteins involved in myeloid stem cell development and leukaemia as well as provide new targets for rational pathogenesis-based therapies of leukaemia and cancer.
Behre
Gerhard
G
Department of Internal Medicine III, University Hospital Grosshadern, Ludwig-Maximilians-University Munich and GSF-National Research Center for Environment and Health, Germany. gerdbehre@aol.com
Reddy
Venkateshwar A
VA
Tenen
Daniel G
DG
Hiddemann
Wolfgang
W
Zada
Abdul A Peer
AA
Singh
Sheo M
SM
D022762:CCAAT-Enhancer-Binding Proteins / Q000032:analysis / Q000502:physiology; D002460:Cell Line; D050676:Core Binding Factor Alpha 2 Subunit / Q000032:analysis / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D018507:Gene Expression Regulation, Developmental; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000378:metabolism; D022423:Myeloid Cells / Q000502:physiology*; D009363:Neoplasm Proteins / Q000032:analysis / Q000502:physiology*; D015514:Oncogene Proteins, Fusion / Q000032:analysis / Q000502:physiology; D010455:Peptides / Q000032:analysis; D040901:Proteomics; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000502:physiology; D000075142:RUNX1 Translocation Partner 1 Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013234:Stem Cells / Q000502:physiology*; D015534:Trans-Activators / Q000032:analysis / Q000502:physiology; D014157:Transcription Factors / Q000032:analysis / Q000502:physiology*; D000099232:Proto-Oncogene Protein Spi-1
D022762:CCAAT-Enhancer-Binding Proteins; D050676:Core Binding Factor Alpha 2 Subunit; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D010455:Peptides; D011518:Proto-Oncogene Proteins; D000075142:RUNX1 Translocation Partner 1 Protein; D015534:Trans-Activators; D014157:Transcription Factors; D000099232:Proto-Oncogene Protein Spi-1; C107844:AML1-ETO fusion protein, human