Application of vitamin B(12)-targeting site on Lactobacillus helveticus B-1 to vitamin B(12) assay by chemiluminescence method.
Lactobacillus helveticus B-1 is assumed to have a vitamin B(12)-targeting (or B(12)-binding) site on the cells, since the binding reaction of vitamin B(12) with L. helveticus B-1 cells proceeded instantly and quantitatively. This reaction is specific to complete B(12) compounds, cobalamins, and can be used for a vitamin B(12) assay method by chemiluminescence. The calibration graph was linear from 0.1 to 10.0 ng/mL. The B(12) contents in oyster and sardine were 75.9 and 39.4 microg/100g, respectively. These values were very close to those obtained using a chemilumi-ADVIA Centaur immunoassay system with intrinsic factor and to those obtained by microbiological assays.
Sato
Kazuyoshi
K
Graduate School of Education, Hiroshima University, 1-1-1 Kagamiyama, Higashi-Hiroshima 739-8524, Japan. ksato@hiroshima-u.ac.jp
Muramatsu
Kumi
K
Amano
Setsumi
S
D000166:Acridines / Q000378:metabolism; D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D005399:Fishes; D007118:Immunoassay / Q000379:methods; D007778:Lactobacillus / Q000166:cytology / Q000378:metabolism*; D016014:Linear Models; D008163:Luminescent Measurements; D008828:Microbiological Techniques / Q000379:methods; D010125:Ostreidae / Q000737:chemistry; D015203:Reproducibility of Results; D014805:Vitamin B 12 / Q000032:analysis* / Q000378:metabolism*
D000166:Acridines; D000345:Affinity Labels; D014805:Vitamin B 12