Expression of survivin during liver regeneration.
Survivin functions to suppress cell death and regulate cell division, and is observed uniquely in tumor cells and developmental cells. However, the expression and regulation of survivin in non-transformed cells are not well elucidated. Therefore, we investigated the expression of survivin in a murine liver regeneration model after partial hepatectomy and intraperitoneal carbon tetrachloride (CCl(4)) injection. We found that the expression of survivin transcript and protein were markedly elevated with the onset of DNA synthesis and remained elevated during G2 and M phases during liver regeneration. In a normal mouse liver cell line, over-expression of survivin resulted in a decrease in the G0/G1 phase and an increase in the S and G2/M phases, resulting in Rb phosphorylation. These findings suggest that survivin is dramatically expressed in a cell cycle-dependent manner during liver regeneration and provide a new insight into the regulation of cell proliferation and differentiation.
Deguchi
Masatoshi
M
First Department of Internal Medicine, Mie University School of Medicine, 2-174, Edobashi, Tsu, Mie, Japan.
Shiraki
Katsuya
K
Inoue
Hidekazu
H
Okano
Hiroshi
H
Ito
Takeshi
T
Yamanaka
Takenari
T
Sugimoto
Kazushi
K
Sakai
Takahisa
T
Ohmori
Shigeru
S
Murata
Kazumoto
K
Furusaka
Akihiro
A
Hisatomi
Hisashi
H
Nakano
Takeshi
T
D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D002478:Cells, Cultured; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis*; D016195:G2 Phase / Q000502:physiology; D022781:Hepatocytes / Q000378:metabolism*; D051034:Inhibitor of Apoptosis Proteins; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins; D008938:Mitosis / Q000502:physiology; D009363:Neoplasm Proteins; D010766:Phosphorylation; D016160:Retinoblastoma Protein / Q000378:metabolism; D016196:S Phase / Q000502:physiology; D000077022:Survivin; D014162:Transfection
C107489:BIRC5 protein, human; D002868:Chromosomal Proteins, Non-Histone; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D016160:Retinoblastoma Protein; D000077022:Survivin