Determination of the Secondary Structure of the king Cobra Neurotoxin CM-11.
The king cobra neurotoxin CM-11 is a small protein with 72 amino acid residues. After its complete assignments of (1)H-NMR resonance's were obtained using various 2D-NMR technologies, including DQF-COSY, clean-TOCSY and NOESY, the secondary structure was analysed by studying the various NOEs extracted from the NOESY spectra and the distribution of chemical shifts. The secondary structure was finally determined by MCD as follows: a triple-strand antiparallel beta sheet with I20-W26, R37-A43 and V53-S59 as its beta strands, a short alpha helix formed by W30-G35 and four turns formed by P7-K1O, C14-G17, K50-V53 and D61-N64.
Pang
Yu-Xi
YX
Applied Spectroscopy Lab, Changchun Institute of Applied Chemistry, the Chinese Academy of Sciences, Changchun 130022, China.
Liu
Wei-Dong
WD
Liu
Ai-Zhuo
AZ
Pei
Feng-Kui
FK