A single nucleotide polymorphism in the alternative oxidase gene among rice varieties differing in low temperature tolerance.
Alternative oxidase (AOX) is encoded in a multigene family, and multiple isoforms have been observed in various plant species. We found for the first time an allelic variation in the same AOX locus. On SDS-gel blots of callus protein of rice (Oryza sativa L.), varieties without the QTL for low temperature tolerance showed a 32-kDa AOX band, whereas those with the QTL showed a 34-kDa band. The variation was attributed to the substitution of Lys(71) for Asn(71) caused by a single nucleotide polymorphism between alleles of OsAOX1a, and was tightly linked to the presence of the QTL.
Abe
Fumitaka
F
Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan.
Saito
Koji
K
Miura
Kiyoyuki
K
Toriyama
Kinya
K
D001216:Asparagine / Q000235:genetics; D002874:Chromosome Mapping; D014644:Genetic Variation; D008239:Lysine / Q000235:genetics; D024101:Mitochondrial Proteins; D012275:Oryza / Q000502:physiology*; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013696:Temperature; D000099188:Alternative Oxidase
D001216:Asparagine; D008239:Lysine; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D011994:Recombinant Proteins; D000099188:Alternative Oxidase