A novel method for the determination of carbonyl groups in cellulosics by fluorescence labeling. 2. Validation and applications.
Fluorescence labeling with the marker carbazole-9-carboxylic acid [2-(2-aminooxyethoxy)ethoxy]amide was shown to be a promising approach toward the accurate determination of carbonyls in cellulosic materials. Combined with gel permeation chromatography in DMAc/LiCl with fluorescence/multiple-angle laser light scattering/refractive index detection, the method yields carbonyl profiles relative to the molecular weight of the cellulosic material. The derivatization procedure can be carried out either homogeneously in DMAc/LiCl or advantageously as heterogeneous derivatization in aqueous buffer. The heterogeneous carbonyl group determination, offering shorter reaction times and increased simplicity as compared to the homogeneous approach, was comprehensively validated. The carbonyl content in numerous dissolving pulps of different provenience has been determined, including pulps with carbonyl contents additionally increased by oxidative treatment. The method was also applied to follow bleaching sequences and oxidative treatments of pulps.
Röhrling
Jürgen
J
Christian-Doppler-Laboratory, University of Agricultural Sciences Vienna, Muthgasse 18, A-1190 Vienna, Austria.
Potthast
Antje
A
Rosenau
Thomas
T
Lange
Thomas
T
Borgards
Andrea
A
Sixta
Herbert
H
Kosma
Paul
P
D000447:Aldehydes / Q000032:analysis; D002482:Cellulose / Q000737:chemistry*; D002850:Chromatography, Gel; D029964:Fagus / Q000737:chemistry; D005456:Fluorescent Dyes / Q000592:standards*; D007659:Ketones / Q000032:analysis; D008722:Methods; D014934:Wood
D000447:Aldehydes; D005456:Fluorescent Dyes; D007659:Ketones; D002482:Cellulose