Influence of aging and calorie restriction on MAPKs activity in rat kidney.
Mitogen-activated protein kinases (MAPK), which include the extracellular signal-related kinases (ERK), the c-Jun N-terminal kinases (JNK), and the p38 MAPK, are important regulatory proteins by which a wide variety of extracellular signals are transduced into intracellular sites. Recent studies reported that mitogenic signal transduction in various cell types are exquisitely sensitive to reactive oxygen species (ROS) and the celluar redox status. In the present study, we investigated the activation of MAPK activity by aging and calorie restriction (CR) in rat kidneys isolated from Fischer 344 rats, ages 6, 12, 18, and 24 months fed ad libitum (AL) and CR diets. Results showed that the aging process strongly enhanced all three of the MAPK activities studied, ERK, JNK, and p38 MAPK, in parallel to increased ROS status. In contrast, we observed CR to markedly suppress the age-related activation of MAPKs. Based on these data, we concluded that an age-related increase in MAPK activity is associated with increased ROS, which was effectively suppressed by the anti-oxidative action of CR.
Kim
Hyon Jeen
HJ
Department of Pharmacy, College of Pharmacy, Pusan National University, Gumjung-ku, Pusan 609-735, South Korea.
Jung
Kyung Jin
KJ
Yu
Byung Pal
BP
Cho
Chong Gun
CG
Chung
Hae Young
HY
D021581:Active Transport, Cell Nucleus; D000375:Aging / Q000378:metabolism*; D000818:Animals; D002149:Energy Intake; D007668:Kidney / Q000201:enzymology*; D008297:Male; D048055:Mitogen-Activated Protein Kinase 8; D048056:Mitogen-Activated Protein Kinase 9; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species; D018808:Transcription Factor AP-1 / Q000378:metabolism
D017382:Reactive Oxygen Species; D018808:Transcription Factor AP-1; D048056:Mitogen-Activated Protein Kinase 9; D048055:Mitogen-Activated Protein Kinase 8; D020928:Mitogen-Activated Protein Kinases