A model of isolated autologously hemoperfused porcine slaughterhouse kidneys.
BACKGROUND
The rapidly evolving field of transplantation research with a focus on ischemic and reperfusion injuries has gained importance since the methodology of organ preservation significantly limits graft survival. Numerous models of isolated perfused kidneys have been established in the past years but limitations such as organ size, perfusate and ethical standards have restricted a widespread research in this area.


METHODS
A model of hemoperfused isolated porcine slaughterhouse kidneys was established which encompasses the advantages of autologous blood as optimal perfusate and a reduction of animal experiments.


RESULTS
The size and geometry of the porcine kidney is more comparable to human conditions and various renal functions, blood parameters and morphology can easily be accessed in the present model. Stable organ function can be maintained over 2 h with an amount of 500-1,000 ml of autologous blood which is metabolically controlled via a dialysis system.


CONCLUSIONS
In summary, the present model describes a new and economic approach for targeting renal function in transplantation models by combining autologous blood as optimal perfusate with a well-defined organ geometry and function and slaughterhouse animals as a source.
Grosse-Siestrup
Christian
C
Department of Experimental Animal Sciences, Biomedical Research Center, Charité Faculty of Medicine, Humboldt University of Berlin, Augustenburger Platz 1, D-13353 Berlin, Germany. christian.grosse-siestrup@charite.de
Unger
Volker
V
Fehrenberg
Claudia
C
v  Baeyer
Hans
H
Fischer
Axel
A
Schäper
Frank
F
Groneberg
David A
DA
D000818:Animals; D001804:Blood Transfusion, Autologous; D005260:Female; D006464:Hemoperfusion; D066298:In Vitro Techniques; D007511:Ischemia / Q000473:pathology / Q000628:therapy; D007668:Kidney / Q000098:blood supply* / Q000293:injuries / Q000473:pathology; D016030:Kidney Transplantation; D008297:Male; D008954:Models, Biological; D009926:Organ Preservation; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control; D034421:Sus scrofa