Vgamma9Vdelta2 T cells recovered from eyes of patients with Behçet's disease recognize non-peptide prenyl pyrophosphate antigens.
The phenotype and antigen-specificity of T cells expanded by mitogenic stimulation from intra-ocular fluid (IOF) samples of affected eyes of six Behçet's disease (BD) patients, and seven patients with other uveitis entities, were determined. High numbers of gammadelta T cells, predominantly Vgamma9Vdelta2 T cells, were only detected in the IOF-derived TCL of three BD patients. Whereas no TCL responded to heat shock protein (HSP) 65 kDa, reactivity to isopentyl pyrophosphate (IPP) and related non-peptide prenyl pyrophosphates (PPP) was restricted to the gammadelta T cell containing TCL. Upon IPP stimulation, these TCL secreted IFN-gamma but no IL-4. By single-cell analysis of intracellular IFN-gamma production and CD69 expression the IOF-derived IPP-specific T cells were identified as CD4(-)CD8(-) gammadelta T cells. The data presented suggest the infiltration of PPP-specific Vgamma9Vdelta2 Th1-like cells into the eye of BD patients with uveitis.
Verjans
Georges M G M
GM
Rotterdam Eye Hospital, PO Box 70030, 3000 LM, Rotterdam, The Netherlands. Verjans@viro.fgg.eur.nl
van Hagen
P Martin
PM
van der Kooi
Alexander
A
Osterhaus
Albert D M E
AD
Baarsma
G Seerp
GS
D000328:Adult; D000368:Aged; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D001082:Aqueous Humor / Q000276:immunology; D001528:Behcet Syndrome / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D017252:CD3 Complex / Q000276:immunology; D016516:CD4-CD8 Ratio; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D002648:Child; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D011756:Diphosphates / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D005123:Eye / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D037181:Lectins, C-Type; D008297:Male; D008875:Middle Aged; D017368:Protein Prenylation / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D016207:Cytokines; D011756:Diphosphates; D000939:Epitopes; D007371:Interferon-gamma; D037181:Lectins, C-Type; D016692:Receptors, Antigen, T-Cell, gamma-delta; D000099192:CD69 Antigens