Methodology for urinary 8-hydroxy-2'-deoxyguanosine analysis by HPLC with electrochemical detection.
The compound 8-hydroxy-2'-deoxyguanosine (8-OHdG), one of the major oxidative DNA base products, is a commonly used biomarker of oxidative DNA damage. The routine measurement of 8-OHdG in biologic samples, and especially in urine, is inherently a challenging analytical problem due to the low levels of the analyte and the complexity of the sample. In this study, a simple and rapid procedure based on one step solid phase extraction (SPE) followed by high-performance liquid chromatography with electrochemical detection (HPLC-ECD) analysis is described. The methodology proposed resulted in a recovery of 74.5+/-12% for urinary 8-OHdG with a detection limit of 5.0 microg l(-1).
De Martinis
Bruno Spinosa
BS
Department of Pathology, Center of Legal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Rua Tenente Catão Roxo 2418, Ribeirão Preto SP14051-140, Brazil. martinis@spider.usp.br
de Lourdes Pires Bianchi
Maria
M
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D015415:Biomarkers / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D004563:Electrochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003404:Creatinine; D003849:Deoxyguanosine