title: DnaK chaperone machine and trigger factor are only partially required for normal growth of Bacillus subtilis.
abstract: While dnaK and tig are the essential components for nascent polypeptide folding in E. coli, deletion did not confer synthetic lethality in B. subtilis, suggesting that under normal growth conditions, another system or mechanism with a specific role prevails. Likewise, survival at high temperature suffered dramatically, resulting from deletion of several sets of heat shock genes, thus during sudden stress various heat shock genes act synergistically to protect the proteins.
authors.affiliation: Department of Bioscience, Tokyo University of Agriculture, Sakuragaoka, Japan.
authors.forename: Dindo Y
authors.initials: DY
authors.lastname: Reyes
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
mesh_terms: D001412:Bacillus subtilis / Q000254:growth & development* / Q000502:physiology*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D017353:Gene Deletion; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000235:genetics; D006358:Hot Temperature; D018832:Molecular Chaperones / Q000502:physiology*; D009876:Operon / Q000235:genetics; D019696:Peptidylprolyl Isomerase / Q000378:metabolism*; D010641:Phenotype; D011489:Protein Denaturation; D017510:Protein Folding
chemical_list: D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; C063842:dnaK protein, E coli; C403324:trigger factor, E coli; D019696:Peptidylprolyl Isomerase