OcaB is required for normal transcription and V(D)J recombination of a subset of immunoglobulin kappa genes.
OcaB, a transcriptional coactivator also known as Bob-1 or OBF-1, was isolated on the basis of its ability to enhance transcription of immunoglobulin (Ig) genes in vitro. Paradoxically, OcaB(-/-) mice showed no apparent deficiency in Ig gene transcription, only cellular immune defects including absence of germinal centers (GC) and decreased numbers of immature B cells; the genes targeted by OcaB were not determined. Here we report that OcaB is essential for V(D)J recombination of a subset of Igkappa genes. We show that OcaB modulates recombination by directly enhancing Igkappa gene transcription in vivo.
Casellas
Rafael
R
Laboratory of Molecular Immunology, New York, NY 10021, USA.
Jankovic
Mila
M
Meyer
Gesa
G
Gazumyan
Anna
A
Luo
Yan
Y
Roeder
Robert
R
Nussenzweig
Michel
M
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D005803:Genes, Immunoglobulin; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D015534:Trans-Activators / Q000235:genetics*; D014158:Transcription, Genetic
D007145:Immunoglobulin kappa-Chains; C490424:Pou2af1 protein, mouse; D015534:Trans-Activators