Nitric oxide synthase inhibitor L-NAME has no effect on 86Rb accumulation in rat renal cortical slices.
The aim of present study was to investigate the effect of the nitric oxide synthase inhibitor L-NAME on the 86Rb uptake in rat renal cortical slices. Rats were divided into three groups: 1. CONTROL. 2. Acute: L-NAME (10 mg/kg i.v.) as a bolus 15 min before the excision of the kidneys. 3. Sub-chronic: L-NAME (10 mg/kg/day) per os for 4 days. Renal cortical slices were incubated for 10, 20, 30, 60, 90, 180 seconds in Krebs-Ringer solution containing 50 kBq 86Rb/100 ml (T = 37 degrees C, PO2 approximately 159 mm Hg). 56Rb accumulation (S/M) was calculated as the ratio of the radioactivity of the cortical slices (S) and the radioactivity of the incubating medium (M). The S/M ratio can be described as a function of time by the following equations.


METHODS
y = 0.265 ln(x) - 0.220, r(xy) = 0.886; acute L-NAME: y = 0.224 ln(x) - 0.171, r(xy) = 0.921; sub-chronic L-NAME: y = 0.331 ln(x) - 0.496, r(xy) = 0.942. (y = S/M, x = t). p < 0.001 in all of the groups, but there is no difference between the groups. In conclusion, L-NAME administered in vivo failed to influence the in vitro 86Rb accumulation in rat renal cortical slices.
Hably
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Semmelweis University, Faculty of Medicine, Department of Physiology, Budapest, Hungary. hably@puskin.sote.hu
Bartha
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D000818:Animals; D004791:Enzyme Inhibitors / Q000008:administration & dosage; D005260:Female; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012414:Rubidium Radioisotopes / Q000493:pharmacokinetics*
D004791:Enzyme Inhibitors; D019275:Radiopharmaceuticals; D012414:Rubidium Radioisotopes; D019331:NG-Nitroarginine Methyl Ester