title: Analysis of sMMO-containing type I methanotrophs in Lake Washington sediment.
abstract: Methane-oxidizing bacteria (methanotrophs) containing soluble methane monooxygenase (sMMO) are of interest in natural environments due to the high co-metabolic activity of this enzyme with contaminants such as trichloroethylene. We have analysed sMMO-containing methanotrophs in sediment from a freshwater lake. Environmental clone banks for a gene encoding a diagnostic sMMO subunit (mmoX) were generated using DNA extracted from Lake Washington sediment and subjected to RFLP analysis. Representatives from the six RFLP groups were cloned and sequenced, and all were found to group with Type I Methylomonas mmoX, although a majority were divergent from known Methylomonas mmoX sequences. Direct hybridization of Lake Washington sediment DNA was carried out using a series of sMMO- and Methylomonas-specific probes to assess the significance of these sMMO-containing Methylomonas-like strains in the sediment. The total sMMO-containing population and the sMMO-containing Methylomonas-like population were estimated to be similar to previous estimates for total methanotrophs and Type I methanotrophs. These results suggest that the major methanotrophic population in Lake Washington sediment consists of sMMO-containing Methylomonas-like (Type I) methanotrophs. The whole-cell TCE degradation kinetics of such a strain, LW15, isolated from this environment, were determined and found to be similar to values reported for other sMMO-containing methanotrophs. The numerical significance of sMMO-containing Methylomonas-like methanotrophs in a mesotrophic lake environment suggests that these methanotrophs may play an important role in methanotroph-mediated transformations, including co-metabolism of halogenated solvents, in natural environments.
authors.affiliation: Department of Microbiology, University of Washington, Box 351750, Seattle, WA 98195, USA.
authors.forename: Ann J
authors.initials: AJ
authors.lastname: Auman
authors.forename: Mary E
authors.initials: ME
authors.lastname: Lidstrom
mesh_terms: D020620:Methylomonas / Q000201:enzymology* / Q000378:metabolism; D015345:Oligonucleotide Probes; D010105:Oxygenases / Q000032:analysis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D014241:Trichloroethylene / Q000378:metabolism
chemical_list: D015345:Oligonucleotide Probes; D014241:Trichloroethylene; D010105:Oxygenases; C021555:methane monooxygenase