Protein Engineering of Insulin: [B9Glu, B10Asp] Human Insulin.
B9Ser and B10His of the insulin B chain are substituted respectively by Glu and Asp using a gapped duplex DNA approach for site-directed mutagenesis. A mutant insulin-[B9Glu, B10Asp] human insulin was obtained. The receptor binding capacity of the mutant insulin is 34.4% as that of porcine insulin. However, the in vivo biological activity of [B9Glu, B10Asp] human insulin is almost as the same as that of porcine insulin.
Liang
Zhen-He
ZH
State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
Liu
Bin
B
Tang
Yue-Hua
YH
Feng
You-Min
YM