title: Coordinated methyl and RNA binding is required for heterochromatin localization of mammalian HP1alpha.
abstract: In mammalian cells, as in Schizosaccharomyces pombe and Drosophila, HP1 proteins bind histone H3 tails methylated on lysine 9 (K9). However, whereas K9-methylated H3 histones are distributed throughout the nucleus, HP1 proteins are enriched in pericentromeric heterochromatin. This observation suggests that the methyl-binding property of HP1 may not be sufficient for its heterochromatin targeting. We show that the association of HP1alpha with pericentromeric heterochromatin depends not only on its methyl-binding chromo domain but also on an RNA-binding activity present in the hinge region of the protein that connects the conserved chromo and chromoshadow domains. Our data suggest the existence of complex heterochromatin binding sites composed of methylated histone H3 tails and RNA, with each being recognized by a separate domain of HP1alpha.
authors.affiliation: Expression Génétique et Maladies, URA 1644 du CNRS, Institut Pasteur, Paris, France. muchardt@pasteur.fr
authors.forename: Christian
authors.initials: C
authors.lastname: Muchardt
authors.forename: Marie
authors.initials: M
authors.lastname: Guilleme
authors.forename: Jacob-S
authors.initials: JS
authors.lastname: Seeler
authors.forename: Didier
authors.initials: D
authors.lastname: Trouche
authors.forename: Anne
authors.initials: A
authors.lastname: Dejean
authors.forename: Moshe
authors.initials: M
authors.lastname: Yaniv
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004330:Drosophila; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000378:metabolism; D006570:Heterochromatin / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006570:Heterochromatin; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D000090266:Chromobox Protein Homolog 5; D012313:RNA; D005982:Glutathione Transferase