IL-15 up-regulates iNOS expression and NO production by gingival epithelial cells.
To investigate the biological activity of epithelial cells in view of host defense, we analyzed the mRNA expression of inducible NOS (iNOS) as well as NO production by human gingival epithelial cells (HGEC) stimulated with IL-15. RT-PCR analysis revealed that HGEC expressed IL-15 receptor alpha-chain mRNA. In addition, stimulation with IL-15 enhanced iNOS expression by HGEC through an increase of both mRNA and protein levels. Moreover, IL-15 up-regulated the production of NO(2)(-)/NO(3)(-), a NO-derived stable end product, from HGEC. The enhanced NO production by IL-15 was inhibited by AMT, an iNOS-specific inhibitor. These results suggest that IL-15 is a potent regulator of iNOS expression by HGEC and involved in innate immunity in the mucosal epithelium.
Yanagita
Manabu
M
Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Japan.
Shimabukuro
Yoshio
Y
Nozaki
Takenori
T
Yoshimura
Naoko
N
Watanabe
Junko
J
Koide
Hiroko
H
Terakura
Mami
M
Saho
Teruyuki
T
Takedachi
Masahide
M
Jang
Myoung-Ho
MH
Kiyono
Hiroshi
H
Murakami
Shinya
S
D002469:Cell Separation; D002478:Cells, Cultured; D015638:Cytochalasin D / Q000494:pharmacology; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005434:Flow Cytometry; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D019409:Interleukin-15 / Q000235:genetics / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D004791:Enzyme Inhibitors; D019409:Interleukin-15; D019384:Nucleic Acid Synthesis Inhibitors; D012333:RNA, Messenger; D015638:Cytochalasin D; D009569:Nitric Oxide; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II