Study on Endogenous Substrates for Calcineurin in Rat Synaptosome.
The inhibition of endogenous calcineurin activity with polyclonal antibody revealed the presence of 11 phosphoprotein substrates for this enzyme in adult rat cerebral cortex synaptosome as studied by high resolution two-dimensional PAGE and autoradiography. The changes of these endogenous substrates for calcineurin from newborn rat and adult rat cerebral cortex synaptosome were analyzed quantitatively by using digital image scanning system based on the 2-D software and simultaneous, comparison with those of whole rat brain was made. Some of them were shown to be expressed at higher levels on postnatal day 3 and believed to be the neuromodulin/GAP-43 as reported in its literature. The important role of calcineurin in nerve growth and development was discussed.
Yan
Li-Jun
LJ
Department of Biology, Beijing Normal University, Beijing 100875, China.
Wei
Qun
Q