[Theoretical validation of local express auto-cytokine therapy].
Theoretical validation for local express autocytokine therapy (LEACKT), used in recent years with good results for the treatment of viral and nonviral diseases of the eyes, is provided. In vitro experiments showed that autologous cytokine complex appearing after stimulation of patient's peripheral whole blood with poludan (polyA:polyY complex, 200 micrograms) includes all three interferon types (alpha, beta, and gamma) and interleukin-8 (IL-8) and tumor necrosis factor (TNF). It was shown for the first time that poludan stimulated the production of IL-8, the main chemotactic cytokine inducing chemotaxis, neutrophil activation, angiogenesis stimulation, and regeneration. This was not paralleled by an increase in the production of TNF, the key mediator of inflammation, whose level in whole blood did not increase. Addition of poludan to cell culture led to a 2-fold decrease of lymphocyte apoptosis in comparison with that after treatment with dexamethasone alone. The results of experiments indicate that LEACKT with poludan is characterized by pronounced antiinflammatory, interferonogenic, regeneratory, and antiapoptosis effects in various diseases of the anterior segment of the eye and is a simple and safe method.
Kasparova
E A
EA
Fadeeva
L L
LL
Pavliuk
A S
AS
Pereverzina
O K
OK
Kasparov
A A
AA
D000293:Adolescent; D000328:Adult; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D017209:Apoptosis; D002478:Cells, Cultured; D002633:Chemotaxis; D003470:Culture Media; D016207:Cytokines / Q000627:therapeutic use*; D003907:Dexamethasone / Q000494:pharmacology; D005128:Eye Diseases / Q000188:drug therapy*; D015817:Eye Infections / Q000188:drug therapy; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007372:Interferons; D007378:Interleukins; D007962:Leukocytes / Q000187:drug effects; D011131:Polyribonucleotides / Q000494:pharmacology*; D012038:Regeneration; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha
D000893:Anti-Inflammatory Agents; D003470:Culture Media; D016207:Cytokines; D007378:Interleukins; D011131:Polyribonucleotides; D014409:Tumor Necrosis Factor-alpha; C044366:poludan; D003907:Dexamethasone; D007372:Interferons