[The influential factors in using cadmium reduction method for measurement of the stable products of NO--nitrates and nitrites].
To establish an efficient assay method for detecting nitric oxide indirectly, we compared the effects of cadmium filing, 5 mmol/L and 80 mmol/L copperized cadmium filing on the measurement of nitrates and nitrites (the stable products of NO) using the cadmium reduction method. The results demonstrated that cadmium filing was the most efficient cadmium preparations (P < 0.001). It's mean reduction rate was 96%, and it showed stronger reduction effectiveness in deproteinization cell culture media. The results indicated that cadmium filing has strong anti-interference capacity in biological fluid. We recommend the cadmium filing reduction method because it is simple, practical, inexpensive, highly efficient and can be performed in ordinary laboratories.
Gu
L
L
Department of Pathophysiology, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Yang
Z
Z
Jing
D
D
Wang
S
S
D001011:Aorta / Q000166:cytology; D002104:Cadmium; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D009566:Nitrates / Q000032:analysis; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000032:analysis; D019163:Reducing Agents
D009566:Nitrates; D009573:Nitrites; D019163:Reducing Agents; D002104:Cadmium; D009569:Nitric Oxide