Role of nuclear PKC delta in mediating caspase-3-upregulation in Jurkat T leukemic cells exposed to ionizing radiation.
The response of Jurkat T cells to ionizing radiation (IR) includes cell cycle arrest and DNA damage, which lead to the occurrence of apoptosis. Here, we try to elucidate some of the early intracellular signals which control the induction of such a process upon IR exposure, addressing to examine the specific role of several PKC isoforms (delta, epsilon, zeta) and their subcellular distribution. Attention has been focused on the connections between nuclear PKC delta activation and the expression of cell death regulators (Bcl-2 family proteins Bad, Bax and Bcl-2) and cell death effector caspase-3 (CPP32) which lead to the cleavage of cytoskeletal and nuclear proteins and induction of apoptosis. Altogether these results let us to conclude that PKC delta, potentiating the pro-apoptotic effect of caspase 3, plays a key role in the cellular response to IR and thus can be considered a molecular target for therapy.
Cataldi
Amelia
A
Dipartimento di Biomorfologia, Università G.D'Annunzio, Chieti, Italy. cataldi@unich.it
Miscia
Sebastiano
S
Centurione
Lucia
L
Rapino
Monica
M
Bosco
Domenico
D
Grifone
Giovanna
G
Valerio
Valentina Di
VD
Garaci
Francesco
F
Rana
Rosalba
R
D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology* / Q000528:radiation effects*; D003593:Cytoplasm / Q000201:enzymology / Q000528:radiation effects; D004790:Enzyme Induction / Q000528:radiation effects; D006801:Humans; D020287:In Situ Nick-End Labeling; D019169:Jurkat Cells; D009687:Nuclear Proteins / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D051745:Protein Kinase C-delta; D011839:Radiation, Ionizing
D009687:Nuclear Proteins; C497258:PRKCD protein, human; D011493:Protein Kinase C; D051745:Protein Kinase C-delta; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases