A rapid PCR method of genotyping LDL receptor mutations in WHHL rabbits.
WHHL rabbits are a valuable model for the study of human familial hypercholesterolemia and atherosclerosis. To use this animal model, it is often necessary to confirm LDL receptor status in WHHL rabbits. Here, we described a simple and rapid PCR method to detect LDL mutations in WHHL rabbits.
Sun
Huijun
H
Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan.
Usui
Shin-ichi
S
Shiomi
Masashi
M
Watanabe
Teruo
T
Fan
Jianglin
J
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004195:Disease Models, Animal; D005838:Genotype; D006938:Hyperlipoproteinemia Type II / Q000235:genetics; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D011817:Rabbits; D011973:Receptors, LDL / Q000235:genetics*
D017931:DNA Primers; D011973:Receptors, LDL