[Determination of vanillymandelic acid in urine by high performance liquid chromatography].
OBJECTIVE
To develop a high performance liquid chromatography(HPLC) for determining vanillylmandelic acid(VMA) in urine.


METHODS
The analytical column was packed with Nova-Pak C18. Flow rate was 0.9 ml.min-1. The mobile phase was 40 mmol.L-1 NaH2PO4 buffer. The VMA of 30 healthy people in 24 hour urine were tested by the internal standard method and with electrochemical detector.


RESULTS
The linear range was from 5 mumol.L-1 to 200 mumol.L-1. The mean analytical recovery rate was 99.1%. The mean within-day coefficient of variation(CV) was 5.8%, and the mean day-to-day CV was 7.7%. The detection limit was 0.28 mumol.L-1. The VMA of 24 hour urine was 18.13 +/- 6.17 mumol in healthy people.


CONCLUSIONS
The method is suitable for routine determination of VMA in urine.
Zheng
L M
LM
Department of Medical Laboratory, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Tang
Y
Y
Shen
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D014642:Vanilmandelic Acid / Q000652:urine*
D014642:Vanilmandelic Acid