Characterization of the early stages of programmed cell death in maize root cells by using comet assay and the combination of cell electrophoresis with annexin binding.
An emerging topic in plant biology is whether plant cells display similar elements of programmed cell death (PCD) as animal cells do. We have studied cell death in maize roots exposed to cold stress by using fluorescence microscopy, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL), DNA gel electrophoresis, single cell gel electrophoresis (SCGE), cell electrophoresis, and annexin binding techniques. The results showed that cell death in maize root cells triggered by cold stress was accompanied by a subset of features characteristic of animal PCD such as nuclear condensation and fragmentation, and oligonucleosomal DNA fragmentation. In addition to DNA laddering and TUNEL positivity, a "comet" pattern indicative of DNA breakage appeared as short as after one day of treatment. The maize root cell PCD process was also accompanied by an increase in negative surface charge of the dying cells due to exposure of phosphatiolylserine (PS) from inner to outer membrane. After annexin binding, however, the enhanced electrophoretic mobility (EPM) of the dying cells decreased nearly to normal values. This result suggests that the combination between cell electrophoresis and annexin binding provides a quantitative method for monitoring PS exposure during plant PCD.
Ning
Shun-Bin
SB
Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan, P. R. China.
Song
Yun-Chun
YC
Damme Pv
Patrick van
Pv
D017302:Annexins / Q000502:physiology*; D017209:Apoptosis / Q000502:physiology*; D020552:Comet Assay / Q000379:methods; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D020287:In Situ Nick-End Labeling; D010940:Plant Proteins / Q000502:physiology; D018517:Plant Roots / Q000166:cytology*; D011485:Protein Binding; D003313:Zea mays / Q000166:cytology*
D017302:Annexins; D010940:Plant Proteins