title: Cytoskeletal structure of myoblasts with the mitochondrial DNA 3243A-->G mutation and of osteosarcoma cells with respiratory chain deficiency.
abstract: The cytoskeleton, mainly composed of actin filaments, microtubules, and intermediate filaments, is involved in cell proliferation, the maintenance of cell shape, and the formation of cellular junctions. The organization of the intermediate filaments is regulated by phosphorylation and dephosphorylation. We examined cell population growth, apoptotic cell death, and the morphology of cytoskeletal components in myoblast cultures derived from patients with the 3243A-->G mutation in mitochondrial DNA (mtDNA) and from control subjects by means of assays detecting cellular nucleic acids, histone-associated DNA fragments and by immunolabeling of cytoskeletal components. Population growth was slower in the 3243A-->G myoblast cultures, with no difference in the amount of apoptotic cell death. The organization of vimentin filaments in myoblasts with 3243A-->G was disturbed by randomization of filament direction and length, whereas no disturbances were observed in the other cytoskeletal proteins. Vimentin filaments formed large bundles surrounding the nucleus in mtDNA-less (rho(0)) osteosarcoma cells and in osteosarcoma cells after incubation with sodium azide and nocodazole. We conclude that defects in oxidative phosphorylation lead to selective disruption of the vimentin network, which may have a role in the pathophysiology of mitochondrial diseases.
authors.affiliation: Department of Neurology, University of Oulu, Oulu, Finland.
authors.forename: Harri
authors.initials: H
authors.lastname: Rusanen
authors.forename: Johanna
authors.initials: J
authors.lastname: Annunen
authors.forename: Heli
authors.initials: H
authors.lastname: Ylä-Outinen
authors.forename: Aino
authors.initials: A
authors.lastname: Laurila
authors.forename: Juha
authors.initials: J
authors.lastname: Peltonen
authors.forename: Ilmo E
authors.initials: IE
authors.lastname: Hassinen
authors.forename: Kari
authors.initials: K
authors.lastname: Majamaa
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004579:Electron Transport / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D032446:Myoblasts / Q000502:physiology* / Q000648:ultrastructure*; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology; D010085:Oxidative Phosphorylation; D010641:Phenotype; D017354:Point Mutation; D019810:Sodium Azide / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000378:metabolism*
chemical_list: D004272:DNA, Mitochondrial; D004791:Enzyme Inhibitors; D014404:Tubulin; D014746:Vimentin; D019810:Sodium Azide