Screening approach for chiral separation of pharmaceuticals part II. Reversed-phase liquid chromatography.
A screening strategy for the rapid separation of drug enantiomers by reversed-phase liquid chromatography was developed using three cellulose/amylose stationary phases. The key point to achieve enantioselectivity is the control of the compound ionisation. Only two mobile phases, i.e. an acidic phosphate buffer (pH 2.0) containing a chaotropic salt (KPF6) and a borate buffer (pH 9.0) mixed with acetonitrile, are used in the proposed strategy. This strategy was successfully applied to a set of 37 diverse chiral pharmaceuticals. Satisfactory enantioselectivity was achieved for 89% of them.
Perrin
C
C
Department of Pharmaceutical and Biomedical Analysis, Pharmaceutical Institute, Vrije Universiteit Brussel, Belgium.
Matthijs
N
N
Mangelings
D
D
Granier-Loyaux
C
C
Maftouh
M
M
Massart
D L
DL
Heyden
Y Vander
YV
D000143:Acids; D002021:Buffers; D002853:Chromatography, Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents; D013237:Stereoisomerism
D000143:Acids; D002021:Buffers; D004364:Pharmaceutical Preparations; D012997:Solvents