Chromosome 14q may harbor multiple tumor suppressor genes in primary glioblastoma multiforme.
OBJECTIVE
To evaluate whether deletion of chromosome 14q is involved in the carcinogenesis of primary glioblastoma multiforme and to identify possibly common deletion regions. METHJODS: Fourteen fluorescent dye-labeled polymorphic markers were used and polymerase chain reaction-based microsatellite analysis was employed to investigate loss of heterozygosity (LOH) on chromosome 14q in 20 primary glioblastoma multiforme (GBM).


RESULTS
Ten of twenty (50%) GBM displayed LOH at one or more of the markers on chromosome 14q. Five tumors showed either LOH or non-informative on all markers tested. The most frequent LOH was observed at locus D14S65 (57.1%) on 14q32.1, and in the chromosomal region spanning from D14S63 (47.1%) to D14S74 (46.7%) on 14q23-31. None of the informative loci exhibited microsatellite instability.


CONCLUSIONS
Allelic deletion on chromosome 14q plays an important role in the pathogenesis of GBM.   Chromosomal regions at locus D14S65 on 14q32.1 and spanning from D14S63 to D14S74 on 14q23-31 may harbor multiple tumor suppressor genes associated with GBM.
Hu
Jie
J
Department of Neurosurgery Hua Shan Hospital Fudan University Shanghai 200040 China. ly045012@online.sh.cn
Jiang
Chengchuan
C
Ng
Ho-Keung
HK
Pang
Jesse C S
JC
Tong
Carol Y K
CY
D000328:Adult; D000368:Aged; D002883:Chromosomes, Human, Pair 14; D005260:Female; D016147:Genes, Tumor Suppressor; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged