title: Analysis of serum cytokine profile in a holstein heifer with leukocyte adhesion deficiency which survived for long period.
abstract: Serum cytokine levels and their expression of mRNA on neutrophils from a bone marrow (BM) transplanted heifer with leukocyte adhesion deficiency were evaluated. The clinical condition of the affected heifer was relatively stable after BM-transplantation. Persistent hyper gamma-globulinemia and increased serum immunoglobulin G (IgG) concentrations were monitored longitudinally. The concentration of interleukin (IL)-1beta in serum from the affected heifer ranged from 15.8 to 321.7 ng/ml, and maximum concentration occurred at the time which coincided with peak IL-6. Serum levels of IL-6 ranged from 0.32 to 27.9 ng/m l, and they appeared to be associated with the increment of serum IgG in the affected heifer. mRNAs for IL-1beta, IL-6, IL-8 and granulocyte and macrophage colony stimulating factor (GM-CSF) were increased in neutrophils from the affected heifer compared to controls. Persistent hyper gamma-globulinemia of the affected heifer appeared to be associated with enhanced mRNA expression for IL-6 and its serum levels. These findings suggest that humoral immunity of the affected heifer is activated and the production of neutrophils appears to be enhanced under the incapability of beta(2) integrin-mediated functions of phagocytic cells.
authors.affiliation: Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Nagahata
authors.forename: Katsuro
authors.initials: K
authors.lastname: Hagiwara
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Higuchi
authors.forename: Rikio
authors.initials: R
authors.lastname: Kirisawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iwai
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D006942:Hypergammaglobulinemia / Q000097:blood / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood*; D007375:Interleukin-1 / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D016209:Interleukin-8 / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D009504:Neutrophils / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D016207:Cytokines; D007074:Immunoglobulin G; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor