IF3, a novel cell-differentiation factor, highly expressed in murine liver and ovary.
The IF3 gene was isolated by expression cloning from a cDNA library of mouse oocytes. This gene was revealed to have no homology to any known gene and its cDNA encodes a 202-amino acid protein that contains a signal-peptide sequence. Moreover, an IF3 isoform, IF3(2), was expressed in both liver and ovary. Its cDNA encoded a 92-amino acid protein contains a signal-peptide sequence, which may be an alternative splice and frameshift form of IF3. The mRNA of IF3s was expressed in oocytes, ovary, and liver. Moreover, the gene expression of IF3s was regulated in a development-dependent manner in preimplantation-embryo and liver. Both IF3(1) and IF3(2) isoforms induced the differentiation of 2T3 and ATDC5 cells to the osteogenic and chondrogenic phenotype, respectively, suggesting that IF3s may modulate the differentiation status. Our findings suggest that IF3 may be one of the secreted factors that regulate oogenesis and certain liver functions.
Mano
Hiroshi
H
Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan. h-mano@josai.ac.jp
Nakatani
Sachie
S
Aoyagi
Rika
R
Ishii
Rina
R
Iwai
Yuka
Y
Shimoda
Nanako
N
Jincho
Yuko
Y
Hiura
Hitoshi
H
Hirose
Minako
M
Mochizuki
Chikako
C
Yuri
Motoko
M
Hyock Im
Ryang
R
Funada-Wada
Ulala
U
Wada
Masahiro
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D005260:Female; D015723:Gene Library; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009865:Oocytes / Q000737:chemistry / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D020033:Protein Isoforms; D021382:Protein Sorting Signals; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
C467579:IF3 protein, mouse; D020033:Protein Isoforms; D021382:Protein Sorting Signals; D011506:Proteins