Protein Engineering of Insulin: [B9 Glutamic Acid] Human Insulin.
B9Ser of insulin B chain was substituted by Glu using site-directed mutagenesis to obtain a fast-acting insulin-[B9Glu] human insulin. The receptor binding capacity and in vivo biological activity of [B9Glu] human insulin are 21% and 40% as those of porcine insulin respectively.
Liu
Bin
B
National Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
Liang
Zhen-He
ZH
Tang
Yue-Hua
YH
Zhang
Xin-Tang
XT
Zhu
Shang-Quan
SQ
Feng
You-Min
YM