title: Purification of Protein Disulfide-isomerase from Human Liver and Preparation of Its Antiserum.
abstract: Protein disulfide-isomerase has been isolated from human liver. The preparative procedure involved heat treatment, (NH(4))(2)SO(4) precipitation, CM-Sephadex C50 and DEAE-fast flow chromatography. The enzyme was homogenous and had a molecular mass of 60 kD or 120 kD as determined by sodium dodecy1 sulphate electro-phoresis and gel filtration respectively, indicating that the enzyme was a 120 kD dimmer with a subunit with molecular mass of 60 kD. The enzyme activity was as high as 830 U/g.protein as measured by the reactivation of "scrambled" ribonuclease. The antiserum of high titer was prepared by immunizing New Zealand rabbit with a mixture of the protein disulfide-isomerase and adjuvant.
authors.affiliation: Zhujiang Hospital, First Military University, Guangzhou 510280, China.
authors.forename: Bing-Yi
authors.initials: BY
authors.lastname: Wu
authors.forename: Gui-Xiang
authors.initials: GX
authors.lastname: Feng
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Feng
authors.forename: Fu-Qin
authors.initials: FQ
authors.lastname: Wang