title: Structural analysis of an extracellular polysaccharide bioflocculant of Klebsiella pneumoniae.
abstract: The glycoside composition and sequence of an extracellular polysaccharide flocculant of Klebsiella pneumoniae H12 was analyzed. GC and HPLC analysis of the acid-hydrolysate identified its constituent monosaccharides as D-Glc, D-Man, D-Gal, and D-GlcA in an approximate molar ratio of 3.9:1.0:2.3:3.6. To analyze the glycoside sequence, the polysaccharide was partially hydrolyzed by acid and enzyme treatment. GC, HPLC, TLC, MALDI-TOF/MS, and 1H- and 13C- NMR spectroscopy characterized the obtained oligosaccharides. The results clarified the partial structure of H12 polysaccharide as a linear polymer of a unit of pentasaccharide with a side chain of one D-GlcA to D-Glc moiety (see below). Although the existence of other sequences or other constituent glycosides could not be fully excluded, H12 polysaccharide must be a novel types as such a complicated unit for a polymer has not so far been reported. The partial structure of a H12 polysaccharide flocculant is also discussed in this report. [structure: see text]
authors.affiliation: Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology, Narashino, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Takiguchi
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Yazawa
authors.forename: Kuniho
authors.initials: K
authors.lastname: Nakata
authors.forename: Tatsuaki
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Kurane
mesh_terms: D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D005285:Fermentation; D006868:Hydrolysis; D007711:Klebsiella pneumoniae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002241:Carbohydrates; D011134:Polysaccharides