title: Identification of Trichophyton rubrum by PCR  fingerprinting.
abstract: OBJECTIVE
  To observe the unique DNA profile and the relationship between DNA profile and phenotype of Trichophyton rubrum,and establish an effective molecular method to identify T. rubrum.
  
  
  METHODS
  Three primers, including (GACA)(4), (GTG)(5) and M13 core sequence (5'-GAGGGTG-GCGGTTCT-3'), were used to distinguish variations among 20 clinical isolates of T. rubrum and Trichophyton mentagrophytes.
  
  
  RESULTS
  Different PCR-fingerprinting was seen between T. rubrum and T. mentagrophytes using three different primers. 2 stains of T. rubrum were identified among 6 supposed T. mentagrophytes.
  
  
  CONCLUSIONS
  T. rubrum can be distinguished using PCR, and (GACA)(4) is the most suitable primer.
authors.affiliation: Cyptoccucus Laboratory, Department of Dermatology, Changzheng Hospital Second Military Medical University, Shanghai 200003, China. hmzhu__cn@yahoo.com.cn
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhu
authors.forename: Hai
authors.initials: H
authors.lastname: Wen
authors.forename: Wanqing
authors.initials: W
authors.lastname: Liao
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D014249:Trichophyton / Q000235:genetics / Q000302:isolation & purification*