[Dot immunoassay with colloidal silver particles as a marker of specific antigen for testing human sera for brucellosis].
Whether the dot immunoassay is suitable for the detection of Brucella antibodies in human sera by using a colloidal silver-labeled Brucella specific antigen as a diagnostic tool is assessed. The antigen was the B. abortus 19BA protein polysaccharide complex isolated by Brucella acetic acid hydrolysis. The dot immunoassay is easy-to-use, cost-effective, highly sensitive, and therefore of more informative value in detecting Brucella antigens than routine serological tests (Huddleson test, Wright agglutination test, passive hemagglutination test, long-term complement fixation test, and Coombs test). It requires no use of expensive equipment and reagents.
Zagoskina
T Iu
TIu
Kalinovskiĭ
A I
AI
Merinov
S P
SP
Dokorina
A A
AA
Golubinskiĭ
E P
EP
D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000737:chemistry; D002003:Brucella abortus / Q000276:immunology / Q000302:isolation & purification*; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis*; D003102:Colloids; D006801:Humans; D015151:Immunoblotting / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D003102:Colloids; D012834:Silver