title: Preparation and sensitization of polystyrene latex beads by some antigens and antibodies. Factors affecting sensitivity and specificity of latex agglutination tests.
abstract: Polystyrene latex beads were polymerized at two different pH (7.3 and 9.5) and their ability to create latex agglutination systems were studied. Sensitization of these latexes by DNA, antibodies to C-reactive protein, myoglobin, and human IgG. was conducted. Sensitivity and specificity of these systems were compared. Practical recommendations are given for preparation of such systems.
authors.affiliation: H. Ch. Buniatyan Institute of Biochemistry of the Armenian NAS, Yerevan, Republic of Armenia. biochem@ipia.sci.am
authors.forename: V K
authors.initials: VK
authors.lastname: Gasparyan
mesh_terms: D000818:Animals; D000906:Antibodies; D000941:Antigens; D002097:C-Reactive Protein / Q000276:immunology; D002417:Cattle; D004247:DNA; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007074:Immunoglobulin G / Q000276:immunology; D007841:Latex Fixation Tests / Q000592:standards*; D008863:Microspheres; D009211:Myoglobin / Q000276:immunology; D011137:Polystyrenes / Q000138:chemical synthesis* / Q000592:standards; D011485:Protein Binding; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D000941:Antigens; D007074:Immunoglobulin G; D009211:Myoglobin; D011137:Polystyrenes; C024075:styrofoam; D002097:C-Reactive Protein; D004247:DNA