Temperature non-homogeneity in rapid airflow-based cycler significantly affects real-time PCR.
Zuna
J
J
2nd Department of Pediatrics, 2nd Medical School, Charles University Prague, Czech Republic. jan.zuma@lfmotol.cuni.cz
Muzikova
K
K
Madzo
J
J
Krejci
O
O
Trka
J
J
D000392:Air Movements; D004247:DNA / Q000032:analysis; D019544:Equipment Failure Analysis; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D001613:beta 2-Microglobulin / Q000235:genetics
D016159:Tumor Suppressor Protein p53; D001613:beta 2-Microglobulin; D004247:DNA