title: Ubiquinone-1 Induces External Deamino-NADH Oxidation in Potato Tuber Mitochondria.
abstract: The addition of ubiquinone-1 (UQ-1) induced Ca2+-independent oxidation of deamino-NADH and NADH by intact potato (Solanum tuberosum L. cv Bintje) tuber mitochondria. The induced oxidation was coupled to the generation of a membrane potential. Measurements of NAD+-malate dehydrogenase activity indicated that the permeability of the inner mitochondrial membrane to NADH and deamino-NADH was not altered by the addition of UQ-1. We conclude that UQ-1-induced external deamino-NADH oxidation is due to a change in specificity of the external rotenone-insensitive NADH dehydrogenase. The addition of UQ-1 also induced rotenone-insensitive oxidation of deamino-NADH by inside-out submitochondrial particles, but whether this was due to a change in the specificity of the internal rotenone-insensitive NAD(P)H dehydrogenase or to a bypass in complex I could not be determined.
authors.affiliation: Department of Plant Physiology, Lund University, Box 117, S-221 00 Lund, Sweden.
authors.forename: I. M.
authors.initials: IM
authors.lastname: Moller
authors.forename: T. H.
authors.initials: TH
authors.lastname: Roberts
authors.forename: A. G.
authors.initials: AG
authors.lastname: Rasmusson