In planta protein-protein interactions assessed using a nanovirus-based replication and expression system.
The multipartite genome of the nanovirus Faba bean necrotic yellows virus, which consists of one gene on each DNA component, was exploited to construct a series of virus-based episomal vectors designed for transient replication and gene expression in plants. This nanovirus based expression system yields high levels of protein which allows isolation of recombinant protein and protein complexes from plant tissues. As examples, we demonstrated in planta interaction between the nanovirus F-box protein Clink and SKP1, a constituent of the ubiquitin-dependent protein turnover pathway. Thus, replicative nanovirus vectors provide a simple and efficient means for in planta characterization of protein-protein interaction.
Aronson
Marie N
MN
Institut des Sciences du Végétale, CNRS, 91198 Gif sur Yvette, France.
Complainville
Arnaud
A
Clérot
Danielle
D
Alcalde
Hélène
H
Katul
Lina
L
Vetten
H Josef
HJ
Gronenborn
Bruno
B
Timchenko
Tatiana
T
D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D017791:Geminiviridae / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics; D025682:Nanovirus / Q000235:genetics / Q000254:growth & development*; D010944:Plants / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D012231:Rhizobium / Q000235:genetics / Q000254:growth & development; D044786:S-Phase Kinase-Associated Proteins; D014779:Virus Replication / Q000235:genetics*
D018797:Cell Cycle Proteins; D044786:S-Phase Kinase-Associated Proteins