Action of Rubus coreanus extract on systemic and local anaphylaxis.
The effect was investigated of the aqueous extract of Rubus coreanus Miq. (Rosaceae) fruits (RCAE) on systemic and local anaphylaxis. RCAE (0.01-1 g/kg) dose-dependently inhibited systemic anaphylaxis induced by compound 48/80 in mice. RCAE (1 g/kg) also significantly inhibited local anaphylaxis activated by anti-DNP IgE. Pretreatment with RCAE at the same concentration before systemic anaphylaxis reduced the plasma histamine levels in a dose-dependent manner. RCAE (0.001-1 mg/mL) dose-dependently inhibited the histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. The level of cAMP in RPMC, when RCAE was added, significantly increased, compared with that of the normal control. Moreover, RCAE (0.01-1 mg/mL) had a significant inhibitory effect on anti-DNP IgE-induced tumour necrosis factor-alpha production from RPMC. These results indicate that RCAE may possess antianaphylactic action.
Shin
Tae-Yong
TY
College of Pharmacy, Woosuk University, Chonju, Chonbuk, 565-701, Republic of Korea. tyshin@core.woosuk.ac.kr
Kim
Sang-Hyun
SH
Lee
Eun-Soon
ES
Eom
Dong-Ok
DO
Kim
Hyung-Min
HM
D000707:Anaphylaxis / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry; D006632:Histamine / Q000097:blood; D006636:Histamine Release / Q000187:drug effects; D007073:Immunoglobulin E / Q000187:drug effects / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D010537:Peritoneum / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027824:Rosaceae; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D010936:Plant Extracts; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D000242:Cyclic AMP