title: OSBPa, a predicted oxysterol binding protein of Dictyostelium, is required for regulated entry into culmination.
abstract: The oxysterol binding proteins (OSBPs) are believed to control cholesterol homeostasis but their precise mechanism of action is not well understood. The Dictyostelium osbA gene encodes a predicted OSBP, OSBPa, which lacks the PH domain that in most other OSBPs directs targetting to the Golgi. OSBPa instead localises selectively to the cell periphery and also, in some cells, to the perinuclear region. OSBPa null strains form normal fruiting bodies but are defective in the regulation of the transition from slug migration to culmination. Thus a plasma membrane-enriched OSBP family member is essential for correct regulation of the slug-fruiting body switch.
authors.affiliation: School of Life Sciences, University of Dundee, Wellcome Trust Biocentre, Dow Street, DD1 5EH, Dundee, UK.
authors.forename: Masashi
authors.initials: M
authors.lastname: Fukuzawa
authors.forename: Jeffrey G
authors.initials: JG
authors.lastname: Williams
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D003600:Cytosol / Q000378:metabolism; D004023:Dictyostelium / Q000254:growth & development / Q000502:physiology*; D020868:Gene Silencing; D006056:Golgi Apparatus / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D011987:Receptors, Steroid / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D000098825:Oxysterol Binding Proteins
chemical_list: D015800:Protozoan Proteins; D011987:Receptors, Steroid; D000098825:Oxysterol Binding Proteins