title: Interaction of low-molecular-weight chitosan with mimic membrane studied by electrochemical methods and surface plasmon resonance.
abstract: Chitosan has shown its potential as a non-viral gene carrier and an adsorption enhancer for subsequent drug delivery to cells. These results showed that chitosan acted as a membrane perturbant. However, there is currently a lack of direct experimental evidence of this membrane perturbance effect, especially for chitosans with low molecular weight (LMW). In this report, the interaction between a lipid (didodecyl dimethylammonium bromide; DDAB) bilayer and chitosan with molecular weight (MW) of 4200 Da was studied with cyclic voltammetry (CV), electrochemical impedance spectroscopy and surface plasmon resonance (SPR). A lipid bilayer was formed by fusion of oppositely charged lipid vesicles on a mercaptopropionic acid (MPA)-modified gold surface to mimic a cell membrane. The results showed that the LMW chitosan could disrupt the lipid bilayer, and the effect seemed to be in a concentration-dependent manner.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry and National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin 130022, Changchun, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Cui
authors.forename: Xiurong
authors.initials: X
authors.lastname: Yang
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000493:pharmacokinetics; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000493:pharmacokinetics; D048271:Chitosan; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000379:methods; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008567:Membranes, Artificial; D008970:Molecular Weight; D010539:Permeability; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance
chemical_list: D001672:Biocompatible Materials; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D000644:Quaternary Ammonium Compounds; C046112:didodecyldimethylammonium; D002686:Chitin; D048271:Chitosan