title: The skeletal muscle Ca2+ release channel has an oxidoreductase-like domain.
abstract: We used a combination of bioinformatics, electron cryomicroscopy, and biochemical techniques to identify an oxidoreductase-like domain in the skeletal muscle Ca2+ release channel protein (RyR1). The initial prediction was derived from sequence-based fold recognition for the N-terminal region (41-420) of RyR1. The putative domain was computationally localized to the clamp domain in the cytoplasmic region of a 22A structure of RyR1. This localization was subsequently confirmed by difference imaging with a sequence specific antibody. Consistent with the prediction of an oxidoreductase domain, RyR1 binds [3H]NAD+, supporting a model in which RyR1 has a oxidoreductase-like domain that could function as a type of redox sensor.
authors.affiliation: Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Baker
authors.forename: Irina I
authors.initials: II
authors.lastname: Serysheva
authors.forename: Serap
authors.initials: S
authors.lastname: Sencer
authors.forename: Yili
authors.initials: Y
authors.lastname: Wu
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Ludtke
authors.forename: Wen
authors.initials: W
authors.lastname: Jiang
authors.forename: Susan L
authors.initials: SL
authors.lastname: Hamilton
authors.forename: Wah
authors.initials: W
authors.lastname: Chiu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D003198:Computer Simulation; D020285:Cryoelectron Microscopy; D066298:In Vitro Techniques; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011817:Rabbits; D019837:Ryanodine Receptor Calcium Release Channel / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D019837:Ryanodine Receptor Calcium Release Channel; D009243:NAD; D010088:Oxidoreductases