title: G350 of Escherichia coli RNase P RNA contributes to Mg2+ binding near the active site of the enzyme.
abstract: G350 of Escherichia coli RNase P RNA is a highly conserved residue among all bacteria and lies near the known magnesium binding site for the RNase P ribozyme, helix P4. Mutations at G350 have a dramatic effect on substrate cleavage activity for both RNA alone and holoenzyme; the G350C mutation has the most severe phenotype. The G350C mutation also inhibits growth of cells that express the mutant RNA in vivo under conditions of magnesium starvation. The results suggest that G350 contributes to Mg(2+) binding at helix P4 of RNase P RNA.
authors.affiliation: Department of Biochemistry-SL43, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112-2699, USA.
authors.forename: Terri A
authors.initials: TA
authors.lastname: Rasmussen
authors.forename: James M
authors.initials: JM
authors.lastname: Nolan
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001667:Binding, Competitive; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D004492:Edetic Acid / Q000494:pharmacology; D004722:Endoribonucleases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test; D006151:Guanosine / Q000235:genetics / Q000378:metabolism*; D020035:Holoenzymes / Q000235:genetics / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016337:RNA, Catalytic / Q000235:genetics* / Q000378:metabolism; D043262:Ribonuclease P
chemical_list: D029968:Escherichia coli Proteins; D020035:Holoenzymes; D012329:RNA, Bacterial; D016337:RNA, Catalytic; D006151:Guanosine; D004492:Edetic Acid; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli; D008274:Magnesium