title: Natural and induced polyploidy in Acacia dealbata Link. and Acacia mangium Willd.
abstract: Seeds were obtained from seven natural populations of Acacia dealbata, three natural populations of A. mangium and a seed orchard of A. mangium, representing the natural range of the two species. Polyploids were discovered in two of the seven populations of A. dealbata. The 2C DNA amount for diploid A. dealbata (2n = 2x = 26) was 1.74 pg, and for diploid A. mangium (2n = 2x = 26) was 1.30 pg. A naturally occurring tetraploid of A. dealbata (2n = 4x = 52) had a 2C DNA amount of 3.41 pg and a naturally occurring triploid genotype had a 2C DNA amount of 2.53 pg. The use of colchicine and oryzalin was investigated as a means of producing higher frequencies of tetraploids of both A. mangium and A. dealbata for incorporation into breeding programmes. Colchicine treatment gave tetraploid frequencies up to 29% for A. dealbata seedlings, and up to 18% for A. mangium seedlings. In contrast, no tetraploid A. mangium was detected following oryzalin treatment, and the low frequencies of tetraploids observed in A. dealbata could be attributed to their natural occurrence.
authors.affiliation: Horticulture Research International, West Malling, Kent, UK. david.blakesley@hri.ac.uk
authors.forename: David
authors.initials: D
authors.lastname: Blakesley
authors.forename: Annabel
authors.initials: A
authors.lastname: Allen
authors.forename: Till K
authors.initials: TK
authors.lastname: Pellny
authors.forename: Andy V
authors.initials: AV
authors.lastname: Roberts
mesh_terms: D000045:Acacia / Q000145:classification* / Q000187:drug effects / Q000235:genetics*; D032461:Chromosomes, Plant / Q000187:drug effects / Q000235:genetics; D003078:Colchicine / Q000494:pharmacology; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D004136:Dinitrobenzenes / Q000494:pharmacology; D005434:Flow Cytometry; D011123:Polyploidy; D013424:Sulfanilamides
chemical_list: D018744:DNA, Plant; D004136:Dinitrobenzenes; D013424:Sulfanilamides; C012465:oryzalin; D003078:Colchicine