The Interaction of Recombinant Androgen Receptor with the Androgen Responsive Element.
A 1 119 bp (1105 to 2224) fragment of androgen receptor (AR) cDNA, named AR1 (containing the whole DNA binding domain, the hinge site region and the partial hormone binding domain) was constructed into an expression vector pGEX. The GST-ARI fusion protein was expressed in E. coli induced by IPTG and purified from glutathione-Sepharose-4B affinity column. With a known ARE of the C3(l) gene as positive probe, the expressed product was proved to be AR with high ARE-binding affinity by gel shifting assay(EMSA) and in vitro Dnase I footprinting analysis.
Liu
Jun
J
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
Zhu
Guo-Zhang
GZ
Xie
Fang
F
Li
Bo-Liang
BL
Zhang
You-Duan
YD
Zhang
Yong-Lian
YL