title: Characterization of cartilagenous tissue formed on calcium polyphosphate substrates in vitro.
abstract: Successful joint resurfacing by tissue-engineered cartilage has been limited, in part, by an inability to secure the implant to bone. To overcome this, we have developed the methodology to form a cartilage implant in vitro consisting of a layer of cartilagenous tissue overlying a porous, biodegradable calcium polyphosphate (CPP) substrate. As bone will grow into the CPP after implantation, it will result in anchorage of the cartilage. In this study, the cartilagenous tissue formed in vitro after 8 weeks in culture was characterized and compared to native articular cartilage. Light microscopic examination of histological sections showed that there was a continuous layer of cartilagenous tissue on, and integrated with the subsurface of, the CPP substrate. The in vitro-formed tissue achieved a similar thickness to native articular cartilage (mean +/- SEM: in vitro = 0.94 +/- 0.03 mm; ex vivo = 1.03 +/- 0.01 mm). The cells in the in vitro-formed tissue synthesized large proteoglycans (Kav +/- SEM: in vitro = 0.27 +/- 0.01; ex vivo = 0.27 +/- 0.01) and type II collagen similar to the chondrocytes in the ex-vivo cartilage. The in vitro-formed tissue had a similar amount of proteoglycan (GAG microg/mg dry wt.: in vitro = 198 +/- 10; ex vivo = 201 +/- 13) but less collagen than the native cartilage (hydroxyproline microg/mg dry wt.: in vitro = 21 +/- 1; ex vivo = 70 +/- 8). The in vitro-formed tissue had only about 3% of the load-bearing capacity and stiffness of the native articular cartilage, determined from unconfined mechanical compression testing. Although low, this was within the range of properties reported by others for tissue-engineered cartilage. It is possible that the limited load-bearing capacity is the result of the low collagen content and further studies are required to identify the conditions that will increase collagen synthesis.
authors.affiliation: Department of Pathology and Laboratory Medicine, Mount Sinai Hospital and University of Toronto, 600 University Avenue, Suite 600-9, Toronto, Ontario, Canada M5G 1X5.
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Waldman
authors.forename: Marc D
authors.initials: MD
authors.lastname: Grynpas
authors.forename: Robert M
authors.initials: RM
authors.lastname: Pilliar
authors.forename: Rita A
authors.initials: RA
authors.lastname: Kandel
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002130:Calcium Phosphates / Q000737:chemistry*; D002358:Cartilage, Articular / Q000166:cytology* / Q000378:metabolism; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D066298:In Vitro Techniques; D009929:Organ Size; D011509:Proteoglycans / Q000378:metabolism
chemical_list: D002130:Calcium Phosphates; D011509:Proteoglycans; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; D003094:Collagen; D004247:DNA; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous