title: Stimulation of Barley Plasmalemma H+-ATPase by Phytotoxic Peptides from the Fungal Pathogen Rhynchosporium secalis.
abstract: A small family of necrosis-inducing peptides has been identified as virulence factors of Rhynchosporium secalis, a fungal pathogen of barley (Hordeum vulgare L.) Two members of this family, NIP1 and NIP3, were found to stimulate the phosphohydrolyzing activity of the Mg2+-dependent, K+-stimulated H+-ATPase of plasma membrane vesicles isolated from barley leaves by partitioning in an aqueous two-phase system. Stimulation of enzyme activity was saturated by 10 to 15 [mu]M fungal protein. Another member of the peptide family, NIP2, did not affect the enzyme, indicating that it has a different mode of action.
authors.affiliation: Max-Planck-Institut fur Zuchtungsforschung, Department of Biochemistry, Carl-von-Linne-Weg 10, D-5000 Koln 30, Federal Republic of Germany.
authors.forename: L.
authors.initials: L
authors.lastname: Wevelsiep
authors.forename: E.
authors.initials: E
authors.lastname: Rupping
authors.forename: W.
authors.initials: W
authors.lastname: Knogge