title: The regions of securin and cyclin B proteins recognized by the ubiquitination machinery are natively unfolded.
abstract: The proteins securin and cyclin B are destroyed in mitosis by the ubiquitin/proteasome system. This destruction is important to mitotic progression. The N-terminal regions of these proteins contain the sequence features recognized by the ubiquitination system. We have demonstrated using circular dichroism and 1-D and 2-D nuclear magnetic resonance that these rather substantial regions are natively unfolded. Based on these findings, we propose a model that helps to explain previously enigmatic observations.
authors.affiliation: Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, 601 Elmwood Ave., Rochester, NY 14618, USA.
authors.forename: Cathleen J
authors.initials: CJ
authors.lastname: Cox
authors.forename: Kaushik
authors.initials: K
authors.lastname: Dutta
authors.forename: Edward T
authors.initials: ET
authors.lastname: Petri
authors.forename: William C
authors.initials: WC
authors.lastname: Hwang
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Lin
authors.forename: Steven M
authors.initials: SM
authors.lastname: Pascal
authors.forename: Ravi
authors.initials: R
authors.lastname: Basavappa
mesh_terms: D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins; D002942:Circular Dichroism; D019926:Cyclin B / Q000737:chemistry* / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*; D017510:Protein Folding; D029701:Saccharomyces cerevisiae Proteins; D064249:Securin; D017386:Sequence Homology, Amino Acid; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D019926:Cyclin B; D005656:Fungal Proteins; D009687:Nuclear Proteins; C578004:PDS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D064249:Securin; D025801:Ubiquitin