A statistical experimental design to study factors affecting enantioseparation of propranolol by capillary electrophoresis with cellobiohydrolase (Cel7A) as chiral selector.
The capillary electrophoretic enantioseparation of rac-propranolol using cellobiohydrolase Tr Cel7A as selector was optimized by an unbiased statistical experimental design. A set of pre-experiments was performed in order to identify critical experimental factors. In the definitive chemometric design pH, ranging from 5 to 7, ionic strength ranging between 0.01 and 0.02 and organic solvent additive in concentration from 1 to 19% v/v were studied. The response surface plot revealed a separation optimum in the pH interval studied. When all parameters were taken into account, a background electrolyte consisting of 0.016 M bistris-acetate buffer with pH 6.5 and 17% v/v acetonitrile gave the optimum separation. The significance of the statistical design was confirmed by the generally good agreement obtained between predicted response and actual experimental data.
Harang
Valérie
V
Product Analysis, Pharmaceutical and Analytical R&D, AstraZeneca, Södertälje, Sweden. valerie.harang@astrazeneca.com
Tysk
Marcus
M
Westerlund
Douglas
D
Isaksson
Roland
R
Johansson
Gunnar
G
D002021:Buffers; D002480:Cellulase / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008956:Models, Chemical; D009994:Osmolar Concentration; D011433:Propranolol / Q000302:isolation & purification*; D012107:Research Design / Q000706:statistics & numerical data*; D012997:Solvents; D013237:Stereoisomerism
D002021:Buffers; D012997:Solvents; D011433:Propranolol; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase