title: An improved general approach for cloning and characterizing telomeres: the protozoan parasite Trypanosoma cruzi as model organism.
abstract: We here describe a general strategy for cloning and characterizing telomeric and sub-telomeric regions of the human protozoan parasite Trypanosoma cruzi. The use of a bacterial artificial chromosome vector and a telomeric adaptor produced stable telomeric recombinant clones with inserts ranging from 5 to 25 kb. Analysis of these recombinants provided unique landmarks for chromosomal mapping and sequencing and enabled us to derive a more accurate picture of T. cruzi telomeric organization.
authors.affiliation: Laboratorio de Genetica Molecular, Instituto de Biologia Experimental Universidad Central de Venezuela calle Suapure, Colinas de Bello Monte, Apdo 47525, Caracas 1041-A, Venezuela.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Chiurillo
authors.forename: Marcia R M
authors.initials: MR
authors.lastname: Santos
authors.forename: Jose
authors.initials: J
authors.lastname: Franco Da Silveira
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Ramírez
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA; D016615:Telomere / Q000235:genetics*; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins