title: Protein Engineering of Insulin: [B9 Glutamic Acid] Human Insulin.
abstract: B9Ser of insulin B chain was substituted by Glu using site-directed mutagenesis to obtain a fast-acting insulin-[B9Glu] human insulin. The receptor binding capacity and in vivo biological activity of [B9Glu] human insulin are 21% and 40% as those of porcine insulin respectively.
authors.affiliation: National Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Zhen-He
authors.initials: ZH
authors.lastname: Liang
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Tang
authors.forename: Xin-Tang
authors.initials: XT
authors.lastname: Zhang
authors.forename: Shang-Quan
authors.initials: SQ
authors.lastname: Zhu
authors.forename: You-Min
authors.initials: YM
authors.lastname: Feng