title: The expression of tenascin mRNA in human temporomandibular joint specimens.
abstract: The expression of mRNA of tenascin in the temporomandibular joint (TMJ) disc and synovial membrane was examined in 20 human TMJ samples from patients with internal derangement of the TMJ and 10 control specimens by in situ hybridization technique using paraffine-embedded tissue, and antisense and sense cRNA probes. In control specimens, tenascin mRNA was not expressed. However, we were able to find tenascin mRNA expression in the surgical specimens. In 15 of 20 samples, ranging numbers of synovial cells expressed tenascin mRNA in the hypertrophic synovial membranes. Also, in 6 of 20 samples, tenascin mRNA was identified in fibroblasts. In four specimens, vascular endothelial cells were positive for the mRNA. In internal derangement cases, histopathological findings are often found such as synovitis, new capillary growth and fibrosis. The present study demonstrates that tenascin is produced specifically in synovial cells, vascular endothelial cells and fibroblasts affected in the portion of TMJ with internal derangement.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Fukui Prefectural Hospital, Fukui-city, Japan. ao73j30l@yu.incl.ne.jp
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: S
authors.initials: S
authors.lastname: Fujita
authors.forename: M
authors.initials: M
authors.lastname: Nishida
authors.forename: T
authors.initials: T
authors.lastname: Iizuka
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Sakakura
mesh_terms: D000328:Adult; D000368:Aged; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D004204:Joint Dislocations / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism*; D019224:Temporomandibular Joint Disc / Q000378:metabolism*; D013705:Temporomandibular Joint Disorders / Q000378:metabolism*; D019063:Tenascin / Q000096:biosynthesis*
chemical_list: D015347:RNA Probes; D012333:RNA, Messenger; D019063:Tenascin