An internal control for the diagnosis of crown gall by PCR.
The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown gall diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the attainment of at least one amplification product in every PCR reaction if the DNA extracted is of high enough quality to be amplified.
Cubero
J
J
Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Cra Moncada Náquera Km 4,5, Apartado Oficial 46113 Moncada, Valencia, Spain.
van der Wolf
J
J
van Beckhoven
J
J
López
M M
MM
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005188:False Negative Reactions; D005638:Fruit / Q000382:microbiology*; D010941:Plant Tumors / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D011786:Quality Control; D012015:Reference Standards
D017931:DNA Primers; D004269:DNA, Bacterial