title: Eucosterol oligoglycosides isolated from Scilla scilloides and their anti-tumor activity.
abstract: Two new eucosterol oligoglycosides, 15-deoxo-30-hydroxyeucosterol 3-O-alpha-L-rhamnopyranosyl-(1-->2)-[(beta-D-glucopyranosyl-(1-->3)]-beta-D-glucopyranosyl-(1-->2)-alpha-L-arabinopyranosyl-(1-->6)-beta-D-glucopyranoside (scillanoside L-1, 1) and 3beta,31-dihydroxy-17alpha,23-epoxy-5alpha-lanost-8-en-23,26-olactone 3-O-alpha-L-rhamnopyranosyl-(1-->2)-[beta-D-glucopyranosyl-(1-->3)]-beta-D-glucopyranosyl-(1-->2)-alpha-L-arabinopyranosyl-(1-->6)-beta-D-glucopyranoside (scillanoside L-2, 2), were isolated from the bulbs of Scilla scilloides, together with four that were known (3-6), have been isolated from the bulbs of Scilla scilloides. The structures of the new compounds were determined on the basis of spectroscopic and chromatographic methods, and some chemical transformations were discussed. Amongst the isolated compounds, 3 showed the most significant cytotoxicity against tumor cells tested several types with ED(50) value of 1.53-3.06 nM. In vivo experiments, 3 apparently increased the life span of mice bearing Sarcoma 180 tumor cell with T/C value of 239% at dose of 3 mg/kg.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon.
authors.forename: Sang-Myung
authors.initials: SM
authors.lastname: Lee
authors.forename: Hyo-Kon
authors.initials: HK
authors.lastname: Chun
authors.forename: Choong-Hwan
authors.initials: CH
authors.lastname: Lee
authors.forename: Byung-Sun
authors.initials: BS
authors.lastname: Min
authors.forename: Eun-Sook
authors.initials: ES
authors.lastname: Lee
authors.forename: Yung-Hee
authors.initials: YH
authors.lastname: Kho
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000473:pathology; D008958:Models, Molecular; D008968:Molecular Conformation; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D010840:Phytosterols / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D012510:Sarcoma 180 / Q000188:drug therapy / Q000473:pathology; D031445:Scilla / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D009844:Oligosaccharides; D010840:Phytosterols