Highly efficient oligosaccharide synthesis on water-soluble polymeric primers by recombinant glycosyltransferases immobilised on solid supports.
Recombinant beta-1,4-galactosyltranferase (beta 1,4-GalT) and alpha-2,6-sialytransferase (alpha 2,6-SiaT) immobilised covalently with activated Sepharose beads were employed for the practical synthesis of a trisaccharide derivative, Neu-5Ac alpha(2-->6)Gal beta(1-->4)GlcNAc beta-O-(CH2)6-NH2, on a water-soluble primer having GlcNAc residues through a alpha-chymotrypsin-sensitive linker.
Nishiguchi
S
S
Sapporo Laboratory ofr Glycocluster Project, Japan Bioindustry Association, Sapporo 060-0810, Japan.
Yamada
K
K
Fuji
Y
Y
Shibatani
S
S
Toda
A
A
Nishimura
S
S
D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism; D006001:Glycoconjugates / Q000138:chemical synthesis*; D006031:Glycosylation; D009237:N-Acetyllactosamine Synthase / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D011108:Polymers; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry; D012799:Sialyltransferases / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D014531:Uridine Diphosphate Galactose / Q000378:metabolism; D014867:Water; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D006001:Glycoconjugates; D009844:Oligosaccharides; D011108:Polymers; D011994:Recombinant Proteins; D014867:Water; D014531:Uridine Diphosphate Galactose; D012685:Sepharose; D009237:N-Acetyllactosamine Synthase; D012799:Sialyltransferases; D002918:Chymotrypsin; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase