Cell-Surface display of heterologous proteins: From high-throughput screening to environmental applications.
A variety of expression systems for the display of either short peptides or fully folded proteins on E.coli and, to a lesser extent, on Gram-positive bacteria have been developed. The expression of proteins on the surface of microbial cells has proved extremely important for numerous applications ranging from combinatorial library screening and protein engineering, to whole cell biocatalysts and adsorbants for bioremediation purposes.
Chen
Wilfred
W
Department of Chemical and Environmental Engineering, University of California, Riverside 92521, USA.
Georgiou
George
G
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D030562:Databases, Protein; D019020:Directed Molecular Evolution / Q000379:methods*; D004787:Environmental Pollution / Q000517:prevention & control; D015964:Gene Expression Regulation, Bacterial; D015723:Gene Library; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D019216:Metals, Heavy / Q000378:metabolism; D015202:Protein Engineering / Q000295:instrumentation / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014183:Transplantation, Heterologous
D001426:Bacterial Proteins; D008565:Membrane Proteins; D019216:Metals, Heavy; D011994:Recombinant Proteins