Beneficial effects of Hippophae rhamnoides L. on nicotine induced oxidative stress in rat blood compared with vitamin E.
The aim of this study was to determine the effects of Hippophae rhamnoides L. extract (HRe-1) and also vitamin E as a positive control on nicotine-induced oxidative stress in rat blood, specifically alterations in erythrocyte malondialdehyde (MDA) level, activities of some erythrocyte antioxidant enzymes, and plasma vitamin E and A levels. The groups were: nicotine (0.5 mg/kg/d, intraperitoneal, i.p.); nicotine+vitamin E (75 mg/kg/d, intragastric, i.g.); nicotine+HRe-1 (1 ml/kg/d, i.g.); and control group (receiving only vehicles). There were 8 rats per group and the supplementation period was 3 weeks. Nicotine-induced increase in erythrocyte MDA level was prevented by both HRe-1 and vitamin E. Nicotine-induced decrease in erythrocyte superoxide dismutase (SOD) activity was prevented by HRe-1, but not vitamin E. HRe-1 increased the erythrocyte glutathione peroxidase (GSH-Px) activity compared with nicotine and the vitamin E groups. Catalase activity was not affected. Vitamin E supplementation increased plasma vitamin E level. Plasma vitamin A level was higher in both vitamin E and HRe-1 supplemented groups compared with nicotine and control groups. The results suggest that HRe-1 extract can be used as a dietary supplement, especially by people who smoke, in order to prevent nicotine-induced oxidative stress.
Suleyman
Halis
H
Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
Gumustekin
Kenan
K
Taysi
Seyithan
S
Keles
Sait
S
Oztasan
Nuray
N
Aktas
Omer
O
Altinkaynak
Konca
K
Timur
Handan
H
Akcay
Fatih
F
Akar
Sedat
S
Dane
Senol
S
Gul
Mustafa
M
D000818:Animals; D005638:Fruit; D005979:Glutathione Peroxidase / Q000097:blood; D031281:Hippophae; D008315:Malondialdehyde / Q000097:blood; D009538:Nicotine / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood; D014810:Vitamin E / Q000494:pharmacology*
D010936:Plant Extracts; D014810:Vitamin E; D008315:Malondialdehyde; D009538:Nicotine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase