Comparative study on ELISA, IFA and direct methods in diagnosis of giardiasis.
A total of 200 cases aged 1-13 years complaining of symptoms suggestive of giardiasis in addition to thirty normal healthy children as a control group was examined clinically and laboratory using stool examination and detection of Giardia coproantigen in faeces by Dot ELISA and direct IFA. Stool examination detected 39 cases (19.5%) while Dot ELISA and direct IFA detected 49 cases (24.5%) with 100% sensitivity and 93.8% specificity. The common clinical findings were diarrhea, abdominal cramps, anorexia, underweight, pallor and manifestations of vitamin deficiency. The results were discussed.
Rashid
Samia M
SM
Department of Parasitology, Benha Faculty of Medicine, Benha Branch of Zagazig University, Egypt.
Nagaty
Ibraheim M
IM
Maboud
Amina I Abdel
AI
Fouad
Mahmoud A
MA
Shebl
Adel
A
D000293:Adolescent; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000469:parasitology*; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D016829:Giardia lamblia / Q000276:immunology / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis*; D006801:Humans; D007223:Infant; D008297:Male; D010270:Parasite Egg Count; D012680:Sensitivity and Specificity
D000953:Antigens, Protozoan