title: Interference of the life cycle of fish nodavirus with fish retrovirus.
abstract: Interference of the life cycle of grouper nervous necrosis virus (GNNV), a member of the Nodaviridae, genus Betanodavirus, by snakehead retrovirus (SnRV) has been studied in vitro. SGF-1, a new fish cell line that is persistently infected with SnRV, was induced by inoculating SnRV into the grouper fin cell line GF-1. Culture supernatants and cell pellets from both GNNV-infected SGF-1 and GF-1 cells were collected and employed for virus productivity analysis. The yields of GNNV RNA and capsid protein in GNNV-infected SGF-1 cells were similar to those in GNNV-infected GF-1 cells. However, when GF-1 cells were used for titration, the titre of the culture supernatant from GNNV-infected SGF-1 cells was much higher than that from GNNV-infected GF-1 cells. The titration result suggested that SnRV enhanced the infection or cytopathic effect (CPE) of GNNV during GNNV and SnRV coinfection of the GF-1 cell titration system, although SnRV cannot induce any CPE in GF-1 cells alone, nor can it increase the yield of GNNV after GNNV superinfection of SGF-1 cells. Moreover, GNNV cDNA was detected in both the pellet and the supernatant from GNNV-infected SGF-1 cells. This result indicated that SnRV reverse-transcribed the GNNV single-stranded genomic RNA into cDNA during GNNV superinfection of SGF-1 cells and created a new cDNA stage in the life cycle of the fish nodavirus.
authors.affiliation: Department of Zoology, National Taiwan University, Taipei, Taiwan, Republic of China1.
authors.forename: K W
authors.initials: KW
authors.lastname: Lee
authors.affiliation: Department of Zoology, National Taiwan University, Taipei, Taiwan, Republic of China1.
authors.forename: S C
authors.initials: SC
authors.lastname: Chi
authors.affiliation: Department of Zoology, National Taiwan University, Taipei, Taiwan, Republic of China1.
authors.forename: T M
authors.initials: TM
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D015153:Blotting, Western / Q000379:methods; D002460:Cell Line; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D005393:Fish Diseases / Q000821:virology; D005399:Fishes / Q000821:virology*; D030381:Nodaviridae / Q000235:genetics / Q000502:physiology*; D012327:RNA Virus Infections / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D012190:Retroviridae / Q000502:physiology*; D014762:Viral Interference
chemical_list: D000956:Antigens, Viral; D003470:Culture Media; D012367:RNA, Viral