An improved general approach for cloning and characterizing telomeres: the protozoan parasite Trypanosoma cruzi as model organism.
We here describe a general strategy for cloning and characterizing telomeric and sub-telomeric regions of the human protozoan parasite Trypanosoma cruzi. The use of a bacterial artificial chromosome vector and a telomeric adaptor produced stable telomeric recombinant clones with inserts ranging from 5 to 25 kb. Analysis of these recombinants provided unique landmarks for chromosomal mapping and sequencing and enabled us to derive a more accurate picture of T. cruzi telomeric organization.
Chiurillo
Miguel Angel
MA
Laboratorio de Genetica Molecular, Instituto de Biologia Experimental Universidad Central de Venezuela calle Suapure, Colinas de Bello Monte, Apdo 47525, Caracas 1041-A, Venezuela.
Santos
Marcia R M
MR
Franco Da Silveira
Jose
J
Ramírez
Jose Luis
JL
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA; D016615:Telomere / Q000235:genetics*; D014349:Trypanosoma cruzi / Q000235:genetics*
D016054:DNA, Protozoan; D015800:Protozoan Proteins