Expression systems for production of recombinant allergens.
Recombinant allergenic proteins have been produced in a variety of different expression systems. This review gives examples of and compares prokaryotic expression systems, such as Escherichia coli, and eukaryotic systems including the yeasts, Saccharomyces cerevisiae and Pichia pastoris, baculovirus-insect cell systems, mammalian cell systems and plant systems. Important factors to consider in choosing an expression system are discussed and examples of applications are given.
Schmidt
M
M
Department of Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27858, USA. SchmidtM@mail.ecu.edu
Hoffman
D R
DR
D000485:Allergens / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D010843:Pichia / Q000235:genetics; D010944:Plants / Q000235:genetics; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D018411:Spodoptera / Q000235:genetics
D000485:Allergens; D011994:Recombinant Proteins