Polyploidization and centrosome hyperamplification in inflammatory bronchi.
OBJECTIVE
Inflammatory and tumorous bronchi were screened in order to obtain new tumor relevant cytogenetic parameters.


METHODS
Bronchial cells of 32 patients were cultivated by standard cell culture procedures.


METHODS
Tetraploidy and aneuploidy was determined by enumeration of chromosome 7 and 8 versus the number of centrosomes. The resulting data were correlated with histopathological data.


RESULTS
Tetra- and aneuploidy of epithelial cells were detectable in 76% of tumor cell cultures, 75% of high grade inflammatory tissues and 40% of non- and low grade-inflammatory tissues. Additionally, we observed centrosome hyper-amplification and multipolar mitoses not only in the tumor but also in the early stages of inflammation.


CONCLUSIONS
Inflammatory bronchi already show tumor-specific features and may consequently represent the preliminary genetic stage of cancer development in bronchi.
Lothschütz
D
D
Department of Trauma-, Hand- and Reconstructive Surgery, Saarland University, Homburg, Germany.
Jennewein
M
M
Pahl
S
S
Lausberg
H F
HF
Eichler
A
A
Mutschler
W
W
Hanselmann
R G
RG
Oberringer
M
M
D000328:Adult; D000368:Aged; D001984:Bronchial Neoplasms / Q000235:genetics* / Q000473:pathology*; D018385:Centrosome / Q000378:metabolism / Q000473:pathology*; D002869:Chromosome Aberrations; D004171:Diploidy; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007249:Inflammation / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D011123:Polyploidy; D014407:Tumor Cells, Cultured