[Genetic polymorphism at D3S1545 locus in Chengdu Han and Dali Bai populations in China].
The objective of this study was to understand the distribution of the genetic polymorphisms of STR locus D3S1545 in Chinese populations. EDTA-blood specimens were collected from 148 healthy unrelated Han individuals in Chengdu and 96 healthy unrelated Bai individuals in Dali. The DNA samples were extracted using Chelex method, and then they were amplified by PCR technique. PAGE horizontal electrophoresis was used for typing the PCR product. The results there were nine alleles at D3S1545 locus in Chengdu Han population and six alleles at D3S1545 locus in Dali Bai population. The allele frequencies of Chengdu Han population were D3S1545 * 8:0.0642; D3S1545 * 9:0.0034; D3S1545 * 10:0.0068; D3S1545 * 11:0.0203; D3S1545 * 12:0.1824; D3S1545 * 13:0.3075; D3S1545 * 14:0.3243; D3S1545 * 15:0.0844; D3S1545 * 16:0.0068; and 23 genotypes were observed. The allele frequencies of Dali Bai population were D3S1545 * 8:0.00781; D3S1545 * 11:0.0261; D3S1545 * 12:0.1719; D3S1545 * 13:0.3906; D3S1545 * 14:0.2448; D3S1545 * 15:0.0885; and 16 genotypes were observed. The results of test for Hardy-Weinberg equilibrium showed that the genotype distributions observed at the populations were correspondent with the expected. These findings suggest that the allele frequencies at D3S1545 locus in Chengdu Hans have not remarkable different from those in the Bai population of Dali. D3S1545 may be a useful marker for paternity test and personal identification of biological stain.
Wu
J
J
Institute for Forensic Medicine, WCUMS, Chengdu 610041.
Li
Y
Y
Hou
Y
Y
D000483:Alleles; D044466:Asian People; D002893:Chromosomes, Human, Pair 3; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007438:Introns; D009875:Operator Regions, Genetic / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences