title: Thermophilic cytochrome P450 (CYP119) from Sulfolobus solfataricus: high resolution structure and functional properties.
abstract: Crystal structures of a thermostable cytochrome P450 (CYP119) and a site-directed mutant, (Phe24Leu), from the acidothermophilic archaea Sulfolobus solfataricus were determined at 1.5-2.0 A resolution. We identify important crystallographic waters in the ferric heme pocket, observe protein conformational changes upon inhibitor binding, and detect a unique distribution of surface charge not found in other P450s. An analysis of factors contributing to thermostability of CYP119 of these high resolution structures shows an apparent increase in clustering of aromatic residues and optimum stacking. The contribution of aromatic stacking was investigated further with the mutant crystal structure and differential scanning calorimetry.
authors.affiliation: RIKEN Harima Institute/Spring-8, 1-1-1 Kouto, Mikazuki-cho, Sayo, Hyogo, Japan.
authors.forename: Sam-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Yamane
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Adachi
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Shiro
authors.forename: Kara E
authors.initials: KE
authors.lastname: Weiss
authors.forename: Shelley A
authors.initials: SA
authors.lastname: Maves
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Sligar
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins; D018360:Crystallography, X-Ray; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D006358:Hot Temperature; D006860:Hydrogen Bonding; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010105:Oxygenases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016945:Sulfolobus acidocaldarius / Q000201:enzymology*; D013816:Thermodynamics
chemical_list: D019843:Archaeal Proteins; D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; C408874:CYP119 protein, Sulfolobus solfataricus