[Experimental study on Jurkat cell apoptosis induced by Boswellia carterii Birdw extractive].
OBJECTIVE
To study the influence of Boswellia carterii Birdw(BCB) extractive of different concentrations on human Jurkat cell apoptosis at different time points.


METHODS
Agarose gel electrophoresis, transmission electron microscope(TEM) and flow cytometry(FCM) were used for observing DNA ladders, morphology of Jurkat cells and cell cycle, respectively.


RESULTS
BCB induced apoptosis of Jurkat cells and typical DNA ladders; TEM demonstrated the presence of apoptosis Jurkat cells, condensation of cytoplasm, numerous vaculoes in cytoplasm, compaction of the nuclear chromatin and formation of apoptosis dody. The sub-G1 peak was detected by cell cycle analysis. It revealed that G1 phage cells increased and S phage cells decreased.


CONCLUSIONS
The data indicate that BCB extractive induces time- and concentration-dependent apoptosis in Jurkat cell line.
Liu
X
X
Department of Hematology, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Qi
Z H
ZH
D017209:Apoptosis / Q000187:drug effects*; D029743:Boswellia; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells / Q000166:cytology*; D014407:Tumor Cells, Cultured
D004365:Drugs, Chinese Herbal