Inhibition of gamma-radiation induced DNA damage in plasmid pBR322 by TMG, a water-soluble derivative of vitamin E.
Alpha-tocopherol monoglucoside (TMG), a water-soluble derivative of alpha-tocopherol, has been examined for its ability to protect DNA against radiation-induced strand breaks. Gamma radiation, up to a dose of 6 Gy (dose rate, 0.7 Gy/minute), induced a dose-dependent increase in single strand breaks (SSBs) in plasmid pBR322 DNA. TMG inhibited the formation of gamma-radiation induced DNA single strand breaks (SSBs) in a concentration-dependent manner; 500 microM of TMG protected the single strand breaks completely. It also protected thymine glycol formation induced by gamma-radiation in a dose-dependent manner, based on an estimation of thymine glycol by HPLC.
Rajagopalan
Rema
R
Radiation Biology Division, Bhabha Atomic Research Centre, Mumbai 400 085.
Wani
Khalida
K
Huilgol
Nagaraj G
NG
Kagiya
Tsutomu V
TV
Nair
Cherupally K Krishnan
CK
D002839:Chromans / Q000494:pharmacology*; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D005720:Gamma Rays; D006027:Glycosides / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics* / Q000528:radiation effects*; D014810:Vitamin E / Q000031:analogs & derivatives*
C106323:2-(glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol; D002839:Chromans; D006027:Glycosides; D014810:Vitamin E; D004247:DNA