Five age-dependently expressed genes in mouse brain revealed by the fluorescence differential display-PCR technique.
We used a fluorescence differential display-PCR (FDD-PCR) technique to analyze the genes expressed in mouse brains collected at nine different developmental stages ranging from 3 days to 15 months after birth, and 5 age-dependently expressed genes were found. Age-dependent expression of each of these 5 genes was confirmed by quantitative real-time PCR analysis. Of the 5 genes, 4 (B1-B4) had high homology with the nucleotide sequences of cDNA clones of known mouse genes (myelin proteolipid protein, transferrin, embryo cDNA from the RIKEN full-length enriched library, and protein tyrosine phosphatase), and the rest (B5) with expressed sequence tags of an unknown gene. Sequencing analysis of the full-length cDNA constructed based on the B5 sequence demonstrated that the gene product of B5 was identical to G-substrate, a specific substrate for cGMP-dependent protein kinase. The expression patterns of known genes obtained in our study may provide a further opportunity to investigate the biological and physiological roles of the proteins they encode.
Iida
R
R
Department of Forensic Medicine, Fukui Medical University, Matsuoka-cho, Fukui 910-1193, Japan. ireiko@fmsrsa.fukui-med.ac.jp
Yasuda
T
T
Tsubota
E
E
Takatsuka
H
H
Masuyama
M
M
Matsuki
T
T
Kishi
K
K
D000367:Age Factors; D000818:Animals; D001921:Brain / Q000378:metabolism*; D018076:DNA, Complementary / Q000737:chemistry; D005453:Fluorescence; D020869:Gene Expression Profiling; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction / Q000379:methods*
D018076:DNA, Complementary