Capillary electrophoretic separation by double-strand polyaniline-coate capillaries of the advanced glycation endproducts formed from N-alpha-acetyl-L-lysine with reducing sugars.
Capillary electrophoresis using a capillary coated with a double-strand coating of polyaniline:poly(methyacrylate-co-acrylic acid) (PAN:P[MA-AAI) was used to separate advanced glycation endproducts (AGEs) formed at 37 degrees C from model systems containing either glucose (Glc), fructose (Fru), or glyceraldehyde (GA) and N-alpha-acetyl-L-lysine (NALys). The presence of the P(MA-AA) as a second strand in the polymer allows the maintenance of the conductive state of the PAN at a wide pH range. Effects of buffer pH and coating concentration on the electroosmotic flow (EOF) were investigated. More AGE species can be detected for the GA/NALys mixtures using this coated capillary than upon an uncoated capillary. The coating procedure is simple and the stability of the coated capillary is good.
de Sa
Paula F G
PF
Department of Chemistry, University of Rhode Island, Kingston 02882, USA.
Robb
Christina
C
Resende
Elmo
E
McCarthy
Patrick
P
Yang
Sze C
SC
Brown
Phyllis R
PR
Dain
Joel A
JA
D002241:Carbohydrates; D019075:Electrophoresis, Capillary / Q000379:methods*; D017127:Glycation End Products, Advanced / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry*; D010084:Oxidation-Reduction
D002241:Carbohydrates; D017127:Glycation End Products, Advanced; D007202:Indicators and Reagents; C002315:N(alpha)-acetyllysine; D008239:Lysine