Comparison of Dehydrin Gene Expression and Freezing Tolerance in Bromus inermis and Secale cereale Grown in Controlled Environments, Hydroponics, and the Field.
There have been very few reports on the expression of stress-responsive genes in field-grown material. A barley dehydrin cDNA was used to investigate the expression of dehydrin-like transcripts after low-temperature and abscisic acid-induced acclimation of bromegrass (Bromus inermis Leyss) suspension cells and of bromegrass and rye (Secale cereale) plants grown in the field and under controlled environmental conditions. Field-acclimated plants accumulated high levels of dehydrin transcripts and were very freezing tolerant. Plants grown in pots and hydroponics under controlled environments also accumulated dehydrin transcripts and showed increased freezing tolerance. Simulation of a combined drought and freezing stress in pots resulted in expression of dehydrin-like transcripts comparable to those observed in field-acclimated material.
Robertson
A. J.
AJ
Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 0W0, Canada.
Weninger
A.
A
Wilen
R. W.
RW
Fu
P.
P
Gusta
L. V.
LV