Electrophoretic methods for fractionation of native and heat-denatured bovine beta-lactoglobulin.
We have developed three electrophoretic methods for analytical and preparative separation of native and heat-denatured beta-lactoglobulin. The methods can be applied, e.g., to optimize dairy milk processing, especially in laboratories which are not provided with expensive column chromatographic instruments. The methods consist of native PAGE followed by electroelution. Consequently, the eluted proteins are in solution in a biologically active and native form, and can therefore be used immediately for further analyses.
Valkonen
K H
KH
Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, Sotkamo, Finland. kaija.valkonen@oulu.fi
Marttinen
N
N
Alatossava
T
T
D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006358:Hot Temperature; D007120:Immunochemistry; D007782:Lactoglobulins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D008892:Milk / Q000737:chemistry; D008970:Molecular Weight; D011489:Protein Denaturation
D007782:Lactoglobulins