title: Inhibition of inducible nitric oxide synthesis by catalposide from Catalpa ovata.
abstract: Catalposide (1) and two related iridoids were isolated from the stem of Catalpa ovata (Bignoniaceae) by bioassay guided fractionation. Catalposide (1) significantly inhibited the production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages in a dose-dependent manner. RT-PCR and Western blot analyses demonstrated that catalposide (1) suppressed the expression of inducible nitric oxide synthase (iNOS) gene and iNOS protein. Catalposide (1) also inhibited the activation of LPS-induced NF-kappaB as analyzed by electrophoretic mobility shift assay (EMSA). In addition to the inhibitory effect on NO production in LPS-stimulated RAW 264.7 cells, catalposide (1) significantly inhibited the NO production in cytokine-stimulated human DLD-1 and rat vascular smooth muscle (VSM) cells in a dose-dependent manner.
authors.affiliation: Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Oh
authors.forename: Hyun-Ock
authors.initials: HO
authors.lastname: Pae
authors.forename: Gi-Su
authors.initials: GS
authors.lastname: Oh
authors.forename: Seung Yeob
authors.initials: SY
authors.lastname: Lee
authors.forename: Kyu-Yun
authors.initials: KY
authors.lastname: Chai
authors.forename: Choong Eui
authors.initials: CE
authors.lastname: Song
authors.forename: Tae-Oh
authors.initials: TO
authors.lastname: Kwon
authors.forename: Hun-Taeg
authors.initials: HT
authors.lastname: Chung
authors.forename: Ho-Sub
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000818:Animals; D029402:Bignoniaceae / Q000737:chemistry*; D002460:Cell Line; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005960:Glucosides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D015394:Molecular Structure; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D005960:Glucosides; D012333:RNA, Messenger; C006928:catalposide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse