Interaction of nitric oxide and cell adhesion molecules after 24 hours of complete ureteric obstruction in the rats on a solitary kidney.
We aimed to show whether the administration of exogenous L-Arg would alter the morphological, functional changes and interaction of nitric oxide and cell adhesion molecules such as tenascin and lectin after release of twenty-four hours complete ureteric obstruction in the solitary rat kidney tissue. Forty prepubertal Wistar-Albino rats were separated into 4 groups, each containing 10 rats. In the group 1 (Sham-control, n = 10), right nefrectomy was performed; the left ureter was visualized but not ligated. In the remaining 30 rats, the abdomen was opened and undergone right nephrectomy and the left ureter was completely obstructed. After 24 hours, thirty rats were divided as group 2, 3, and 4, each containing 10 rats. In-group 2, no drug treatments were given. In-group 3 L-Arg (L-arginine methyl ester) was infused immediately after abolishing ureteric obstruction. In-group 4 L-NAME was give separately during L-Arg administration during 30 minutes immediately after abolishing ureteric obstruction. Than, the animals were prepared for functional and histopathological studies. BUN value was decreased significantly in L-Arg group when compared with untreatment and L-NAME groups (p < 0.05, p < 0.001 respectively). Creatinine values were decreased in L-Arg group when compared with untreatment group (p < 0.002). Urine flow and urinary Na value was increased significantly in L-Arg group when compared to other obstruction groups (p < 0.001, p < 0.001). The increase in the number of macrophages in Untreated and L-NAME group were significant (p < 0.001, p < 0.001) when compared to L-Arg group. Immunohistochemical study showed that tenascin and lectin expression was severe in tubulus basal membrane of untreated and L-NAME treated rats. In L-Arg group, tenascin and lectin expression was moderate in tubulus membrane. Our results suggest that the administration of exogenous L-Arg protect the functional and degenerative effects of acute complete obstruction in the solitary kidney tissue of the rats. Nitric oxide cause these positive effects by decreasing preglomerular vascular resistance, regulation of neutrophil function and preventing the expression of cell adhesion molecules such as tenascin and lectin.
Oztürk
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Department of Paediatric Surgery, Dicle University Hospital, Diyarbakir, Turkey. ozturkhayrettin@hotmail.com
Dokucu
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Buyukbayram
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D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D007150:Immunohistochemistry; D007668:Kidney / Q000473:pathology / Q000601:surgery*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism*; D037121:Plant Lectins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D054788:Ribosome Inactivating Proteins; D019063:Tenascin / Q000378:metabolism*; D013997:Time Factors; D014517:Ureteral Obstruction / Q000378:metabolism*
D037121:Plant Lectins; C050600:Sambucus nigra lectins; D019063:Tenascin; D009569:Nitric Oxide; D001120:Arginine; D003404:Creatinine; D054788:Ribosome Inactivating Proteins; D019331:NG-Nitroarginine Methyl Ester