Preparation of Unilamellar Immunoliposomes by Passing Reversed Micelles through Oil-water Interface.
A novel method for the preparation of unilamellar immunoliposomes is introduced. In this method, the aqueous phase is first encapsulated into reverse micelles passing through the oil-water interface, where the monolayer of lecithin embedded with antibody has been formed to self-assemble into immunoliposomes. The main advantages of this method are that the procedure of preparation is simple with high encapsulation yield and it is favorable for large scale production. As shown by negative staining electronic micrograph, the immunoliposomes are unilamellar and 100-500 nm in size. The UV spectra of immunoliposomes solution and lysis assay show that sheep anti-human IgG has been coated on liposomes.
Xiao
Zhong-Dang
ZD
National Laboratory of Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096, China.
Gu
Jian-Hua
JH
Xu
Min-Hua
MH
Lu
Zu-Hong
ZH