[Fungal contamination detection in cornea preservation media].
OBJECTIVE
Fungi can cause major complications following corneal grafting. In this study we aimed to verify the efficiency of a fungal contamination detection protocol for human corneas in preservation before clinical use.


METHODS
The 12 most frequently found species of fungi responsible for keratitis and endophthalmitis were inoculated to preservation medium used by most French eye banks. A protocol used by several centers, including a daily visual control, was followed in order to check that it showed the presence of all microorganisms, particularly slow-growing filamentous fungi.


RESULTS
Every species was detected in a time from 2 to 4 days.


CONCLUSIONS
This microbiological contamination detection protocol of detection for human corneas in organ culture at +31 degrees C, seems to effectively detect the main agents responsible for fungal contamination.
Rousset
A
A
Laboratoire de Parasitologie-Mycologie, CHU Jean Minjoz, 25030 Besançon, France.
Piarroux
R
R
Reboux
G
G
Roubi
N
N
Delbosc
B
B
D002175:Candida / Q000145:classification / Q000302:isolation & purification*; D003315:Cornea / Q000382:microbiology*; D016039:Corneal Transplantation; D005658:Fungi / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D009926:Organ Preservation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013172:Spores, Fungal / Q000302:isolation & purification