Simultaneous estimation of intracellular free Mg2+ and pH by use of a new pH-dependent dissociation constant of MgATP.
In the present technical note for 31P-NMR, we used a new pH-dependent dissociation constant of MgATP, and re-estimated changes in the intracellular free Mg2+ concentration and pH from the chemical shifts of beta- and gamma-ATP during Na+-removal in smooth muscle. We confirmed the role of Na+ - Mg2+ exchange.
Nakayama
Shinsuke
S
Department of Cell Physiology, Nagoya University Graduate School of Medicine, Nagoya, 466-8550 Japan. h44673a@nucc.cc.nagoya-u.ac.jp
Nomura
Hideki
H
Smith
Lorraine M
LM
Clark
Joseph F
JF
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002432:Cecum / Q000378:metabolism*; D006168:Guinea Pigs; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism*; D008954:Models, Biological; D012964:Sodium / Q000494:pharmacology
D006859:Hydrogen; D000255:Adenosine Triphosphate; D012964:Sodium; D008274:Magnesium