A quantitative analysis of perineurial cell basement membrane collagen IV, laminin and fibronectin in diabetic and non-diabetic human sural nerve.
The thickness of the perineurial cell basement membrane was examined in diabetic and non-diabetic human sural nerve. A significant increase in thickness was found in the diabetic group. The nature of this thickening was investigated using immunohistochemistry and image analysis in order to semi-quantify three of the major intrinsic components of the perineurial cell basement membrane: collagen IV, laminin and fibronectin. Amounts of all three components were shown to be increased in the diabetic group, but not significantly so. However, significant linear correlations between fascicle size and perineurial collagen IV, laminin and fibronectin were identified in both diabetic and non-diabetic nerve.
Hill
R E
RE
Department of Biological Sciences, The University of Hull, UK. R.E.Hill@biosci.hull.ac.uk
Williams
R E
RE
D000368:Aged; D001485:Basement Membrane / Q000737:chemistry / Q000473:pathology; D016022:Case-Control Studies; D024141:Collagen Type IV / Q000032:analysis*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005353:Fibronectins / Q000032:analysis; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry / Q000379:methods; D007797:Laminin / Q000032:analysis; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D010525:Peripheral Nerves / Q000737:chemistry* / Q000473:pathology; D018709:Statistics, Nonparametric; D013497:Sural Nerve
D024141:Collagen Type IV; D005353:Fibronectins; D007797:Laminin; D008562:Membrane Glycoproteins