Targeted expression of human serum albumin to potato tubers.
Complementary DNA expression of mature human serum albumin was engineered into potato plants under the transcriptional control of patatin B33 promoter and potato proteinase inhibitor II terminator. Protein secretion was achieved by using the signal sequence from potato proteinase inhibitor II. Recombinant albumin accumulated up to 0.2% of total soluble tuber protein in single transformant lines, regardless of the potato cultivar used. Electrophoretic mobility and N-terminal amino acid sequence analysis of partially purified recombinant albumin confirmed proper processing of an immune responsive recombinant albumin, and revealed that the proteinase inhibitor II signal sequence was correctly removed. No further optimisation of these yields was obtained by HSA expression in patatin antisense plants (line Pas58). Subcellular localisation showed that recombinant protein was successfully targeted to the apoplast. Potato tubers may be used, by applying this technology, to produce other heterologous proteins of interest in the biopharmaceutical industry.
Farran
Inma
I
Institute of Agrobiotechnology CSIC, Agricultural Production Dept., UPNA, Pamplona, Spain.
Sánchez-Serrano
José J
JJ
Medina
Juan F
JF
Prieto
Jesús
J
Mingo-Castel
Angel M
AM
D017209:Apoptosis; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D006801:Humans; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011480:Protease Inhibitors; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012709:Serum Albumin / Q000235:genetics*; D011198:Solanum tuberosum / Q000145:classification / Q000166:cytology / Q000235:genetics*; D014158:Transcription, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D010940:Plant Proteins; D011480:Protease Inhibitors; D012709:Serum Albumin; C034104:proteinase inhibitor II protein, plant