A selective effect of Mpl ligand on mRNA stabilization during megakaryocyte differentiation.
Megakaryocytes, the platelet precursors, are induced to differentiate in response to Mpl ligand. Here we report that stability of the megakaryocyte-specific platelet factor 4 (PF4) mRNA is substantially augmented in the presence of Mpl ligand. This stabilization requires protein synthesis, but the 3'-untranslated region of PF4 mRNA is not sufficient for granting the effect. This cytokine also significantly or mildly stabilizes Mpl receptor or GAPDH mRNAs, respectively, in contrast to a previously reported lack of effect on P2Y(1) receptor mRNA. Our study is the first to suggest that Mpl ligand-induced lineage specification is also determined by message stabilization.
Kaluzhny
Yulia
Y
Department of Biochemistry, K225, Boston University School of Medicine, 715 Albany St., Boston, MA 02118, USA.
Hechler
Beatrice
B
Lu
Jun
J
Nguyen
Hao G
HG
Cataldo
Leah M
LM
Ravid
Katya
K
D024509:3' Flanking Region; D020413:3' Untranslated Regions; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000187:drug effects / Q000235:genetics; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics; D010978:Platelet Factor 4 / Q000187:drug effects / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics; D020871:RNA Stability; D012333:RNA, Messenger / Q000187:drug effects*; D018121:Receptors, Cytokine / Q000235:genetics; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000235:genetics; D058472:Receptors, Purinergic P2Y1; D053628:Receptors, Thrombopoietin; D013926:Thrombopoietin / Q000494:pharmacology*
D020413:3' Untranslated Regions; D009363:Neoplasm Proteins; C546382:P2RY1 protein, human; C546383:P2ry1 protein, mouse; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D018048:Receptors, Purinergic P2; D058472:Receptors, Purinergic P2Y1; D053628:Receptors, Thrombopoietin; C082218:MPL protein, human; D010978:Platelet Factor 4; D013926:Thrombopoietin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases