Regulation of gene expression by melatonin: a microarray survey of the rat retina.
The pineal secretory product melatonin is synthesized by pinealocytes and retinal photoreceptors on a cyclic rhythm, with highest levels occurring at night. Our previous work has demonstrated that melatonin treatment increases the sensitivity of the rat retina to light-induced photoreceptor cell death. This raises the possibility that inappropriate exposure of photoreceptors to melatonin may result in visual impairment, caused by a loss of retinal photoreceptors. We hypothesize that retinal genes whose expression levels are altered in response to melatonin may be involved in processes that contribute to light-induced photoreceptor cell death. To identify retinal genes that are up- or down-regulated in response to melatonin receptor binding, rats were treated with or without melatonin, and the RNA from the neural retinas and retinal pigment epithelium (RPE) were analyzed for differential gene expression by hybridization of labeled cRNA probes to an Affymetrix rat genome microarray set. GeneChip algorithms were applied to measured hybridization intensities of compared samples and showed that in the neural retina, six genes were up-regulated, and eight were down-regulated. In the RPE, 15 genes were up-regulated, and two genes were down-regulated. The protein products of these specific genes are potentially involved in the molecular mechanism of melatonin action in the retina, and may play a role in the effect of melatonin on light-induced photoreceptor cell death. Identification of these candidate genes and their response to melatonin administration may provide a foundation for further studies on gene regulation by melatonin, the function of melatonin in the retina, and the role of circadian signaling in inherited and environmentally induced photoreceptor degenerations.
Wiechmann
Allan F
AF
Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA. allan-wiechmann@ouhsc.edu
D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D008550:Melatonin / Q000494:pharmacology* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D020419:Photoreceptor Cells, Vertebrate / Q000187:drug effects / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000187:drug effects* / Q000378:metabolism*
D012313:RNA; D008550:Melatonin