[Effect of thrombin on cell proliferation and plasminogen activator inhibitor type (PAI-1) expression in human embryonic glomerular mesangial cells].
OBJECTIVE
To explore the effect of thrombin on cell proliferation and plasminogen-activator inhibitor Type 1(PAI-1) expression in human embryonic glomerular mesangial cells.


METHODS
Methyl-tetragolium (MTT) incorporation, fibrin plate assay and Northern blotting hybridization were used to examine the mesangial cells proliferation, protein activity and mRNA expression of PAI-1 in cultured human embryonic mesangial cells induced by thrombin, respectively.


RESULTS
Thrombin enhanced glomerular mesangial cell proliferation, PAI-1 protein activity and mRNA expression in a dose-dependent manner, which could be blocked by hirudin, a specific inhibitor of thrombin.


CONCLUSIONS
Thrombin could enhance mesangial cell proliferation, and up-regulate protein activity and mRNA expression of PAI-1 in cultured human embryonic glomerular mesangial cells. It may play an important role in the pathogenesis of glomerulosclerosis induced by thrombin.
He
Q N
QN
Department of Pediatrics, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
Yi
Z W
ZW
Chen
X M
XM
Fu
B
B
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005333:Fetus; D005920:Glomerular Mesangium / Q000166:cytology*; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013917:Thrombin / Q000494:pharmacology*
D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D013917:Thrombin