title: Apolipoprotein E and other cerebrospinal fluid proteins differentiate ante mortem variant Creutzfeldt-Jakob disease from ante mortem sporadic Creutzfeldt-Jakob disease.
abstract: The ability to perform an ante mortem differential diagnosis of Creutzfeld-Jakob disease (CJD) is aided by several clinical and molecular tests. There is a need for molecular tests which can reliably distinguish ante mortem variant CJD (vCJD) from ante mortem sporadic CJD (spCJD). A proteomics approach employing two-dimensional protein electrophoresis is applied to the study of ante mortem CSF samples obtained in collaboration with the CJD Surveillance Unit and the National Hospital for Neurology and Neurosurgery. The sample set includes two cases of vCJD, three cases of spCJD and three neurologic controls. Preliminary data using a panel of seven molecular markers is able to distinguish vCJD from spCJD using a heuristic clustering algorithm. One of the molecular markers has been identified as apolipoprotein E which appears to be upregulated in the cerebrospiral fluid (CSF) of patients with vCJD as compared to spCJD. Analysis of ante mortem CSF may help to differentiate patients with vCJD from those patients with spCJD.
authors.affiliation: 120 Olin Hall, School of Chemical and Biomolecular Engineering, Ithaca, NY, USA.
authors.forename: Leila H
authors.initials: LH
authors.lastname: Choe
authors.forename: Alison
authors.initials: A
authors.lastname: Green
authors.forename: Richard S G
authors.initials: RS
authors.lastname: Knight
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Thompson
authors.forename: Kelvin H
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D000465:Algorithms; D001057:Apolipoproteins E / Q000134:cerebrospinal fluid*; D015415:Biomarkers / Q000134:cerebrospinal fluid; D016022:Case-Control Studies; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid / Q000145:classification / Q000175:diagnosis*; D003937:Diagnosis, Differential; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D040901:Proteomics
chemical_list: D001057:Apolipoproteins E; D015415:Biomarkers; D002556:Cerebrospinal Fluid Proteins