title: Studies on the Effect of Recombinant Human Tumor Necrosis Factor-alpha on Leukemic Cell Lines by (31)P-NMR.
abstract: 31P nuclear magnetic resonance (NMR) spectroscopy has been used for monitoring cellular metabolism in human lymphoma cell line Molt-4 and human promyelocyte leukemia cell line HL-60. (31)P-NMR spectra of Molt-4 cells and HL-60 cells consist primarily of peaks of phosphomonoesters, inorganic phosphate (Pi), diphosphodiesters and ATP. A decrease in the ATP/Pi ration occurred after treatment of the Molt-4 cells with recombinant human tumor necrosis factor-alpha(rhTHF-alpha). The ATP decreased after treatment of the Hl-60 cells with rhTNF-alpha.
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Rong-Qing
authors.initials: RQ
authors.lastname: Huang
authors.forename: Ze-Han
authors.initials: ZH
authors.lastname: Du
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Feng