Lipopolysaccharide administration increases acid and alkaline phosphatase reactivity in the cardiac muscle.
The effect of lipopolysaccharide (LPS) administration on the in situ distribution of the reaction product of acid phosphatase (AcPase) and alkaline phosphatase (AlPase) activity was examined in the rat cardiac muscle using catalytical cytochemistry. Tissues of the heart were fixed and then incubated in reaction media for detection of AcPase and AlPase reactivity. In normal hearts, reaction product of AcPase activity was observed in lysosomes. AlPase reactivity was detected at the extracellular surface of the capillary endothelilal cells and in their caveolae. Following LPS administration, the number and the size of lysosomes possessing AcPase reactivity as well as their electron density significantly increased. Furthermore, they tended to form groups consisting of three to five lysosomes. Cytochemical reaction 2 and 24 hours after injection was similar. One week later, the reaction returned to its normal pattern. As in the case with AcPase, the first changes of the distribution of the reaction product of AlPase activity were detected 2 hours after injection. The changes included a remarkable increase of the number of enzymatically positive capillaries, intensified cytochemical reaction in endothelial cells, and an increased number of caveolae. Again, no noticeable differences in reactivity were observed 2 and 24 hours after injection and the reaction returned to normal one week later. Collectively, our data indicate that both cardiac AcPase and AlPase are affected early after injection of LPS. Although the pattern of cytochemical reaction of both phosphatases was restored one week later, it is believed that the altered distribution of their reactivity in early periods after LPS administration may be a factor contributing to the development of pathological changes in this organ at a later stage.
Okada
Teruhiko
T
Department of Anatomy and Cell Biology, Kochi Medical School, Kochi 783-8505, Japan. okadat@kochi-ms.ac.jp
Zinchuk
Vadim S
VS
Seguchi
Harumichi
H
D000135:Acid Phosphatase / Q000032:analysis / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D021941:Caveolae / Q000201:enzymology; D003331:Coronary Vessels / Q000201:enzymology; D004730:Endothelium, Vascular / Q000201:enzymology; D005260:Female; D006321:Heart / Q000187:drug effects*; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008070:Lipopolysaccharides; D008247:Lysosomes / Q000201:enzymology; D008854:Microscopy, Electron; D009206:Myocardium / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D008070:Lipopolysaccharides; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase