Single column approach for the liquid chromatographic separation of polar and non-polar glucosinolates from broccoli sprouts and seeds.
Ion-pair and hydrophilic interaction chromatographies are considered to be complementary methods of choice for analyzing intact glucosinolates from broccoli. Ion-pair chromatography resolves non-polar glucosinolates, such as those containing indole moieties, while hydrophilic interaction chromatography is superior for separating polar glucosinolates, such as glucoraphanin and glucoiberin. Reversed-phase separations using hydrophilic endcapped C18-bonded silica and a 50 mM ammonium acetate-methanol gradient mobile phase resolve both polar and non-polar glucosinolates negating the need for switching columns.
West
Leslie
L
Kraft Foods North America, Research and Development, Glenview, IL 60025, USA. lwest@kraft.com
Tsui
Ida
I
Haas
George
G
D001937:Brassica / Q000737:chemistry* / Q000196:embryology; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005961:Glucosinolates / Q000032:analysis*; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D005961:Glucosinolates