title: Distinct ontogenic patterns of overt and latent DGAT activities of rat liver microsomes.
abstract: We have studied the ontogeny of the two functional diacylglycerol acyltransferase (DGAT) activities (overt and latent) during postnatal development in rat liver. We find that the ontogenic patterns of the two are highly distinct. Overt DGAT shows a transient rise in activity up to day 4 postnatally, after which it declines until weaning; thereafter, it increases steadily to reach high adult values that may contribute to the high rates of turnover of cytosolic triacylglycerol (TAG). By contrast, latent DGAT activity increases continuously during the suckling period but falls sharply upon weaning onto chow but not onto a high-fat diet. Rates of TAG secretion by hepatocytes are higher than in the adult during the first 7 days after birth, and are largely dependent on the mobilization of the abundant intrahepatocyte TAG as a source of acyl moieties. When the hepatic steatosis is cleared (after day 7) the TAG secretion rate declines by 80% to reach adult values. Quantification of the content of mRNA for the DGAT1 and DGAT2 genes does not show correlation with either of the DGAT activities. We conclude that post-translational modification may play an important role in the overt and latent distribution of DGAT activity in the liver microsomal membrane.
authors.affiliation: Hannah Research Institute, Ayr, Scotland, United Kingdom.
authors.forename: Ian J
authors.initials: IJ
authors.lastname: Waterman
authors.forename: Nigel T
authors.initials: NT
authors.lastname: Price
authors.forename: Victor A
authors.initials: VA
authors.lastname: Zammit
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D051048:Diacylglycerol O-Acyltransferase; D004041:Dietary Fats / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D008862:Microsomes, Liver / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism; D014886:Weaning
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D012333:RNA, Messenger; D014280:Triglycerides; D000217:Acyltransferases; C494883:Dgat1 protein, rat; D051048:Diacylglycerol O-Acyltransferase