Disruption of protein-protein interactions: design of a synthetic receptor that blocks the binding of cytochrome c to cytochrome c peroxidase.
Synthetic receptor 1 has been found via fluorescence titration to compete effectively with cytochrome c peroxidase for binding cytochrome c (Cc), forming 1:1 Cc:1 complex with a binding constant of (3 +/- 1) x 10(8) M-1, and to disrupt Cc: Apaf-1 complex, a key adduct in apoptosis.
Wei
Y
Y
Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA. weiyen@drexel.edu
McLendon
G L
GL
Hamilton
A D
AD
Case
M A
MA
Purring
C B
CB
Lin
Q
Q
Park
H S
HS
Lee
C S
CS
Yu
T
T
D017209:Apoptosis / Q000187:drug effects; D053477:Apoptotic Protease-Activating Factor 1; D001667:Binding, Competitive / Q000187:drug effects; D002352:Carrier Proteins / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology*; D003574:Cytochrome c Group / Q000037:antagonists & inhibitors* / Q000378:metabolism; D003578:Cytochrome-c Peroxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation; D011506:Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D053477:Apoptotic Protease-Activating Factor 1; D002352:Carrier Proteins; D003574:Cytochrome c Group; D011506:Proteins; D003578:Cytochrome-c Peroxidase