Molecular characterisation of Victorian Newcastle disease virus isolates from 1976 to 1999.
OBJECTIVE
To characterise Newcastle disease virus isolates obtained in Victoria from 1976 to 1999 and identify the diversity of FO cleavage signal.


METHODS
RT-PCR using viral RNA extracted from positive NDV allantoic fluid was performed to amplify a segment of the NDV F and HN genes. Molecular characterisation of the nucleotide and amino acid sequences within the FO cleavage site was undertaken.


RESULTS
All isolates contained 'avirulent FO cleavage signal sequence of varied amino acid composition.


CONCLUSIONS
Molecular characterisation of past and present NDV FO cleavage signal sequences will provide valuable epidemiological information and assist in understanding the genetic origins and relationships of outbreaks.
Peroulis-Kourtis
I
I
Department of Natural Resources and Environment, Victorian Institute of Animal Science, Attwood. irene.peroulis@nre.vic.gov.au
O'Riley
K
K
Grix
D
D
Condron
R J
RJ
Ainsworth
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001717:Birds; D002645:Chickens; D004372:Ducks; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000453:epidemiology* / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics* / Q000302:isolation & purification; D010311:Parrots; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D020310:Struthioniformes; D014739:Victoria / Q000453:epidemiology
D012367:RNA, Viral