High-protein induced renal enlargement is growth hormone independent.
BACKGROUND
Growth hormone (GH) and insulin-like growth factors (IGFs) have been postulated as pathogenic factors in several forms of renal growth, including that induced by high-protein (HP) diets. Compensatory renal growth (CRG) following renal uninephrectomy is strictly GH dependent, while the exact role of GH as a regulating factor in HP induced renal growth has not been fully clarified.


METHODS
To elucidate a possible direct role for GH in HP-induced renal growth, we examined the effect of a newly developed specific GH-receptor (GHR) antagonist (B2036-PEG) on renal growth and renal GH/IGF-system expression in HP-fed mice.


RESULTS
Mice fed a HP diet (45% protein) for one week demonstrated renal hypertrophy and increased renal IGF-I. GH receptor antagonist (GHRA) treatment neither modified renal IGF-I nor abolished the renal hypertrophy. In contrast, however, GHRA administration did modify renal mRNA expression of many members of the GH and IGF systems.


CONCLUSIONS
The major new finding is that HP-induced renal growth in adult mice is GH independent.
van Neck
Johan W
JW
Laboratory of Pediatrics, Subdivision of Molecular Endocrinology, Erasmus University/Sophia Children's Hospital Rotterdam, PO Box 1738, 3000 DR Rotterdam, The Netherlands. vanneck@plch.fgg.eur.nl
Cingel
Vesna
V
van Vliet
Arlène K
AK
Drop
Stenvert L S
SL
Flyvbjerg
Allan
A
D000818:Animals; D001786:Blood Glucose; D001835:Body Weight; D004044:Dietary Proteins / Q000494:pharmacology*; D004435:Eating; D005260:Female; D015870:Gene Expression; D013006:Growth Hormone / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D019382:Human Growth Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D006984:Hypertrophy; D007328:Insulin / Q000097:blood; D018969:Insulin-Like Growth Factor Binding Proteins / Q000097:blood / Q000235:genetics; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000473:pathology* / Q000503:physiopathology; D008099:Liver / Q000033:anatomy & histology / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size; D012333:RNA, Messenger / Q000032:analysis
C422172:B 2036; D001786:Blood Glucose; D004044:Dietary Proteins; D007328:Insulin; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D019382:Human Growth Hormone; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone