Mucociliary transport function and damage of ciliated epithelium.
The influence of epithelial damage on mucociliary transport was studied in relation to the amount of mucus. The mucosal epithelium of mucus-depleted frog palate was dissected and mounted on a plastic plate. Mechanical damages were created on the frog mucosa by pressing a different thickness of boards (2, 3, 5, and 8 mm). Two different amounts of frog mucus (7.9 microL [small amount of mucus (S-mucus]]) and 51.0 microL [large amount of mucus [L-mucus]]) were acpplied on the frog mucosa witih and without mucosal damage. There was no difference in mucociliary transport rate (MTR) on undamaged frog mucosa between S-mucus and L-mucus. However, on the damaged mucosa, MTR of S-miucus was significantly decreased compared with that of L-mucus. Moreover, capability of mucus transportation across the mucosal damage was significantly lower in S-mucus than in L-mnucus. Results indicate that the larger the mucus amount becomes, the more the mucus travels the damaged epithelium. Moreover, MTR of L-mucus was decreased with increasing the degree of epithelial damage. This study indicates that the degree of loss of cilia is an important factor of mucociliarn deceleration.
Yang
Tian-qun
TQ
Department of Otorhinolaryngology, Mie University School of Medicine, Tsu, Japan.
Majima
Yuichi
Y
Guo
Yongqing
Y
Harada
Teruhiko
T
Shimizu
Takeshi
T
Takeuchi
Kazuhiko
K
D000818:Animals; D002923:Cilia / Q000502:physiology*; D004848:Epithelium / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D009079:Mucociliary Clearance / Q000502:physiology*; D009092:Mucous Membrane / Q000648:ultrastructure; D009093:Mucus / Q000502:physiology; D011892:Rana catesbeiana