title: Supercritical fluid extraction and liquid chromatography-electrospray mass analysis of vinblastine from Catharanthus roseus.
abstract: Supercritical fluid extraction using carbon dioxide modified with methanol, methanol-diethylamine, or methanol-triethylamine was used to extract vinblastine from the aerial portions of Catharanthus roseus. An HPLC-electrospray ionization (ESI)/MS analysis method was also developed to quantify the alkaloids in these extracts. Of the supercritical solvents evaluated, carbon dioxide-methanol-triethylamine (80 : 18 : 2) at 80 degrees C and 34.0 MPa greatly improved the supercritical fluid extraction (SFE) yield of vinblastine by as much as 76.4% over methanol extraction, while the other solvent conditions extracted the compound at yields less than 25% that of a methanol extraction. These results were confirmed by the robust HPLC-ESI/MS analytical method developed in this study.
authors.affiliation: GreenTek21 Co., Ltd., Mapo-gu, Seoul, Korea.
authors.forename: Young Hae
authors.initials: YH
authors.lastname: Choi
authors.forename: Ki-Pung
authors.initials: KP
authors.lastname: Yoo
authors.forename: Jinwoong
authors.initials: J
authors.lastname: Kim
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification; D002245:Carbon Dioxide / Q000737:chemistry; D030008:Catharanthus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D014747:Vinblastine / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012997:Solvents; D002245:Carbon Dioxide; D014747:Vinblastine