Effect of salvianolic acids from Radix Salviae miltiorrhizae on regional cerebral blood flow and platelet aggregation in rats.
This study was conducted to observe the effect of salvianolic acids (SA) on regional cerebral blood flow (rCBF) in rats and on platelet aggregation in vitro and in vivo. Cerebral ischemia was produced in rats by occluding of the right middle cerebral artery, together with the right common carotid artery. rCBF was monitored by H2 clearance method with a tissue blood-flow meter. Platelet aggregation induced by collagen, ADP, and AA was measured in vitro and in vivo by platelet aggregometer. Doses of SA at 6 and 10 mg/kg body wt. (i.v.) improved rCBF in rats after ischemia, but had no obvious effect on normal rCBF. In vitro, SA inhibited significantly the platelet aggregation induced by collagen, ADP, and AA with IC50 values of 0.197, 2.22 and 3.29 x 10(3) mg/l, respectively. In vivo, doses of SA at 6 and 10 mg/kg body wt. inhibited significantly the platelet aggregation induced by collagen, and SA at 10 mg/kg body wt. inhibited remarkably platelet aggregation induced by ADP. The results suggest that SA could improve rCBF in the ischemic hemisphere and inhibit platelet aggregation in rats.
Tang
Min-Ke
MK
Pharmacology Department 2, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China. tangmk@imm.ac.cn
Ren
De-Cheng
DC
Zhang
Jun-Tian
JT
Du
Guan-Hua
GH
D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D002109:Caffeic Acids / Q000494:pharmacology*; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D066298:In Vitro Techniques; D007773:Lactates / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D053630:Depsides
D001572:Benzofurans; D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D007773:Lactates; D010975:Platelet Aggregation Inhibitors; C066201:salvianolic acid A; C076944:salvianolic acid B; D053630:Depsides