Microbiological comparative study of isolates of Edwardsiella tarda isolated in different countries from fish and humans.
It is difficult to use tissue culture assays to investigate adherence and other properties of Edwardsiella tarda because the organism is invasive and produces a potent hemolysin. We therefore relied on polymerase chain reaction (PCR) to determine the occurrence of genes for enterotoxins (LT-I, EAST-1), Shiga toxin (Stx-1, Stx-2), cytotoxic necrotizing factors (CNF-1, CNF-2), aerobactin, invasion plasmid of enteroinvasive Escherichia coli, EPEC adherence factor (EAF), intimin (Eae), enterohemolysin (EntHly) and hemolysin (Hly) in 53 isolates of E. tarda from humans and fish from several countries. All isolates were negative for all genes investigated by PCR. Adhesion to and invasion of HeLa cells were determined by using the unusually short incubation time of 1h or 30 min. All isolates adhered and invaded in these tests. Finally, a random amplified polymorphic DNA (RAPD) test distinguished, with a few exceptions, isolates of human and fish origin.
Nucci
C
C
Department of Microbiology and Immunology, Institute of Biology, University of Campinas, SP, Campinas, Brazil.
da Silveira
W D
WD
da Silva Corrêa
S
S
Nakazato
G
G
Bando
S Y
SY
Ribeiro
M A
MA
Pestana de Castro
A F
AF
D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D020609:Edwardsiella tarda / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D005260:Female; D010861:Fimbriae, Bacterial / Q000502:physiology; D005393:Fish Diseases / Q000382:microbiology*; D005399:Fishes; D006367:HeLa Cells; D006801:Humans; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique
D001427:Bacterial Toxins; D004269:DNA, Bacterial