title: The Recombination and Expression of a Chimeric Antibody Light Chain Gene in Cells of Silkworm Bombyx mori.
abstract: A chimeric immunoglobulin light chain gene had been expressed in a baculovirus expression system, which was achieved by infecting the silkworm cells with the recombinant virus r-NPVL2 containing the cDNA of immunoglobulin against a small cell lung cancer. PCR analysis and Southern hybridization proved that the antibody light chain cDNA hand been integrated into the modified BmNPV genome. Expression products of human-mouse chimeric antibody light chain in the silkworm cells were detected by both Western blot and ELISA.
authors.affiliation: Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China.
authors.forename: Guan-Zhen
authors.initials: GZ
authors.lastname: Yang
authors.forename: Xiang-Fu
authors.initials: XF
authors.lastname: Wu
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: Zhang
authors.forename: Si-De
authors.initials: SD
authors.lastname: Li