Direct quantification of human serum albumin in human blood serum without separation of gamma-globulin by the total internal reflected resonance light scattering of thorium-sodium dodecylbenzene sulfonate at water/tetrachloromethane interface.
A direct quantification of human serum albumin (HSA) in blood serum samples without separation is proposed based on the measurements of total internal reflected resonance light scattering (TIR-RLS) at water/tetrachloromethane (H(2)O/CCl(4)) interfaces. In the pH range of 6.37-6.59, the coadsorption of the binary complex of HSA-Th(IV) with sodium dodecylbenzene sulfonate occurs at the H(2)O/CCl(4) interface, forming an amphiphilic layer and displaying greatly enhanced TIR-RLS signals with the maximum peak located at 340-370 nm. The enhanced TIR-RLS intensity is in proportion to the HSA concentration in the range 0.15-1.0 micro gml(-1). The limit of detection is 14.4 ngml(-1). The contents of HSA in blood serum samples were determined with the recovery of 97.1-102.3% and RSD of 0.6-2.9%, which are identical to those obtained according to the spectrofluorimetric method using chrome azurol S.
Feng
Ping
P
Institute of Environmental Chemistry, Southwest Normal University, Chongqing 400715, People's Republic of China.
Huang
Cheng Zhi
CZ
Li
Yuan Fang
YF
D001557:Benzenesulfonates / Q000737:chemistry*; D002251:Carbon Tetrachloride / Q000032:analysis / Q000737:chemistry; D004655:Emulsions; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012031:Refractometry / Q000379:methods; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis*; D013501:Surface-Active Agents / Q000494:pharmacology; D017970:Thorium Compounds / Q000737:chemistry*; D014867:Water / Q000032:analysis; D005719:gamma-Globulins / Q000737:chemistry* / Q000302:isolation & purification
D001557:Benzenesulfonates; D004655:Emulsions; D012709:Serum Albumin; D013501:Surface-Active Agents; D017970:Thorium Compounds; D005719:gamma-Globulins; D014867:Water; C001114:dodecylbenzenesulfonic acid; D002251:Carbon Tetrachloride