Production of polyclonal antiserum specific to the 27.5 kDa envelope protein of white spot syndrome virus.
A truncated version of the white spot syndrome virus (WSSV) 27.5 kDa envelope protein was expressed as a histidine tag fusion protein in Escherichia coli. The bacterial expression system allowed the production of up to 10 mg of purified recombinant protein per liter of bacterial culture. Antiserum from a rabbit immunized with the recombinant protein recognized the 27.5 kDa viral envelope protein of WSSV isolated from different geographic regions. The antiserum did not recognize any of the other known WSSV structural proteins. A sensitive immunodot assay for WSSV was developed using the specific rabbit polyclonal antiserum.
You
Zerong
Z
Department of Microbiology, University of Hawaii, Honolulu 96822, USA. zerong@hawaii.edu
Nadala
E Cesar B
EC
Yang
Jinsheng
J
van Hulten
Marielle C W
MC
Loh
Philip C
PC
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D017756:Aquaculture; D004267:DNA Viruses / Q000737:chemistry* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D007106:Immune Sera / Q000096:biosynthesis* / Q000276:immunology; D015151:Immunoblotting / Q000662:veterinary; D008970:Molecular Weight; D033561:Penaeidae / Q000276:immunology / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011817:Rabbits; D013047:Specific Pathogen-Free Organisms; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D007106:Immune Sera; D014759:Viral Envelope Proteins