title: Sequencing Analysis of Oligodeoxynucleotide by Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry.
abstract: A mixed matrix alpha-cyano 4-hydroxycinnamic acid (alpha-Cyano)/3-hydroxypicolinic acid (3HPA) was found to be a good matrix for the analysis of oligodeoxynucleotides by matrix-assisted laser desorption ionization time-of-flight mass spectrometry ( MALDI-TOF-MS). Using the mixed matrix, a similar sensitivity was obtained for the different oligodeoxynucleotides: d(T)(10), d(A)(10) and d(C)(10). This methodology can be used in combination with the partial digestion of oligodeoxynucleotides by 5'- and 3'-exonucleases as a powerful tool for sequencing analysis of oligonucleotides.
authors.affiliation: National Center of Biomedical Analysis, Beijing 100850, China.
authors.forename: Qing-Jin
authors.initials: QJ
authors.lastname: Yan
authors.forename: Song-Cheng
authors.initials: SC
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Cai
authors.forename: Sheng-Qi
authors.initials: SQ
authors.lastname: Wang
authors.forename: Bao-Zhen
authors.initials: BZ
authors.lastname: Zhu