Molecular cloning and sequence analysis of the Zygosaccharomyces bailii HIS3 gene encoding the imidazole glycerolphosphate dehydratase.
Zygosaccharomyces bailii is a spoilage yeast belonging to the Zygosaccharomyces genus. In recent years these yeasts, due to their exceptional resistance to several stresses, have become more and more interesting as model organisms to study the molecular basis of the said resistance. A Z. bailii cDNA library has been built and the 672 bp nucleotide sequence coding for the HIS3 gene was cloned by complementation of a Saccharomyces cerevisiae his3 mutant strain. The deduced 223 amino acid sequence shares a high degree of homology with His3p homologues in other non-conventional yeast species. The GeneBank Accession No. is AY050224.
Branduardi
Paola
P
Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, Piazza della Scienza, 2-20126 Milano, Italy. paola.branduardi@unimib.it
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D006836:Hydro-Lyases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D017385:Sequence Homology; D020068:Zygosaccharomyces / Q000201:enzymology / Q000235:genetics*
D006836:Hydro-Lyases; C021434:imidazoleglycerolphosphate dehydratase