[Stability in association of the peripheral material with mitotic chromosomes].
The localization of nucleolar proteins (fibrillarin and B-23), and of the protein of interphase nuclear matrix (NMP-65) was studied in the perichromosomal material (CM) after of short hypotonic treatment (15% solution of Henks medium) on cultured pig embryonic kidney cells, followed by restoration of isotonic conditions. It is shown that during hypotonic shock the mitotic chromosomes demonstrate reversible swelling, but their periphery is bounded with a rim of PCM, containing antibodies to fibrillarin and NMP-65, but not to B-23. After returning the cells to the initial isotonic medium, all the three proteins can be detected again on the periphery of chromosomes. It suggests the existence of different stability in the association of free proteins with chromosome bodies. Besides, B-23 and fibrillarin could be visualized in residual nucleoli after a complete extraction of histones and DNA from nuclei.
Kosykh
M I
MI
Department of Cytology and Histology, Biological Faculty, Moscow State University.
Chentsov
Iu S
IuS
D000818:Animals; D002460:Cell Line; D002466:Cell Nucleolus / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D002875:Chromosomes / Q000737:chemistry / Q000378:metabolism*; D054637:Dual-Specificity Phosphatases; D007038:Hypotonic Solutions; D007668:Kidney; D008938:Mitosis; D034521:Nuclear Matrix-Associated Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D013552:Swine
D002868:Chromosomal Proteins, Non-Histone; D007038:Hypotonic Solutions; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins; C047458:fibrillarin; C442320:DUSP5 protein, human; D054637:Dual-Specificity Phosphatases; D017027:Protein Tyrosine Phosphatases