Evaluation of the effect of triterpenes on urinary risk factors of stone formation in pyridoxine deficient hyperoxaluric rats.
Investigations were carried out to evaluate the efficacy of the pentacyclic triterpene, lupeol and its ester, lupeol linoleate, against calcium oxalate urolithiasis in rats. Administration of a pyridoxine deficient diet containing 3% glycollic acid for 21 days led to increased excretion of stone forming constituents such as calcium, oxalate and uric acid. Crystal deposition and subsequent renal tubular damage resulted in increased excretion of the tubular enzymes alkaline phosphatase, lactate dehydrogenase, gamma glutamyl transferase, beta glucuronidase and N-acetyl glucosaminidase along with reduced fibrinolytic enzymes. A reduction in the urinary inhibitory factors magnesium and glycosaminoglycans was also observed. Treatment with lupeol and lupeol linoleate reduced the extent of tubular damage as evidenced from reduced enzymuria and minimized the excretion of stone forming constituents.
Vidya
Lakshminarasimhan
L
Department of Medical Biochemistry, Dr. A.L.M. Post Graduate Institute of Basic Medical Sciences, Taramani, Chennai 600 113, India.
Lenin
Mahimainathan
M
Varalakshmi
Palaninathan
P
D000118:Acetylglucosaminidase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000652:urine; D002129:Calcium Oxalate / Q000652:urine; D005966:Glucuronidase / Q000378:metabolism; D006959:Hyperoxaluria / Q000188:drug therapy*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D010070:Oxalates / Q000652:urine; D053978:Pentacyclic Triterpenes; D011736:Pyridoxine / Q000172:deficiency; D051381:Rats; D017208:Rats, Wistar; D012307:Risk Factors; D014315:Triterpenes / Q000494:pharmacology* / Q000627:therapeutic use / Q000652:urine; D014527:Uric Acid / Q000652:urine; D014545:Urinary Calculi / Q000188:drug therapy*; D005723:gamma-Glutamyltransferase / Q000378:metabolism; D008041:Linoleic Acids; D000094042:Lupanes
D000118:Acetylglucosaminidase; D000469:Alkaline Phosphatase; D002118:Calcium; D002129:Calcium Oxalate; D005966:Glucuronidase; D007770:L-Lactate Dehydrogenase; D010070:Oxalates; D053978:Pentacyclic Triterpenes; D011736:Pyridoxine; D014315:Triterpenes; D014527:Uric Acid; D005723:gamma-Glutamyltransferase; C403497:lupeol linoleate; D008041:Linoleic Acids; D000094042:Lupanes; C010480:lupeol