title: Prevalence of Mycoplasma pneumoniae in children in Diyarbakir, the south-east of Turkey.
abstract: BACKGROUND
  Mycoplasma pneumoniae is a well known causative agent of infection in childhood but clinical presentation may be variable. History and physical examination may not be so helpful for the diagnosis. It is difficult to culture this infectious agent, with the culture technique for the multiplication of the organism for routine investigation hard to obtain. The determination of exposure and prevalence of the disease of any region should be helpful for clinical diagnosis. In this study, we aimed to determine the seroprevalence of M. pneumoniae in children aged 0-14 years in Diyarbakir and establish the distribution of infection prevalence of various age groups of children.
  
  
  METHODS
  Thirty streets were determined according to the cluster method for the 0-6 years age group. In this age group, a total of 180 blood samples were collected; so that there were 30 samples for each age. For children aged 7-14 years, elementary school children were selected. There were 70 elementary schools in the city and 14 schools were randomly selected according to the randomized numbers table with a 20% sample. Finally, 276 blood samples were collected. Mycoplasma pneumoniae was determined by using the enzyme immunoassay method. For this method MELOTEST Mycoplasma IgG reagent was used.
  
  
  RESULTS
  The highest seropositivity rate was encountered at 10 years of age (65%) and lowest was at 2 years of age (0%). The overall M. pneumoniae seropositivity was 27% when all ages were considered together. The first 2 years of age excluded this rate and was 31.9%. A sudden increase of seropositivity was observed at 7 years of age.
  
  
  CONCLUSIONS
  Increasing seroprevalence rates of M. pneumoniae after 2 years of age should alert clinicians to consider the organism in the differential diagnosis of infectious diseases in this age group. The low seroprevalence rates in the first 2 years of age was thought to be due to low risk of community-based spread of the organism in this age group. The most prominent increase in seroprevalence was at 10 years of age. This data indicated that M. pneumoniae should be kept in mind while making differential diagnosis of infections. The spreading of disease and community-based transmission is also important to consider. In our study, seroprevalence rates increased at 6 and 7 years of age, at the beginning of the school age as community-based transmission takes place most commonly in crowded circumstances. In conclusion, M. pneumoniae should be thought as an important infectious agent in childhood for all age groups.
authors.affiliation: Department of Pediatrics, Dicle University Medical School, Diyarbakir, Turkey. mbosnak@dicle.edu.tr
authors.forename: Mehmet
authors.initials: M
authors.lastname: Bosnak
authors.forename: Bunyamin
authors.initials: B
authors.lastname: Dikici
authors.forename: Vuslat
authors.initials: V
authors.lastname: Bosnak
authors.forename: Omer
authors.initials: O
authors.lastname: Dogru
authors.forename: Ilker
authors.initials: I
authors.lastname: Ozkan
authors.forename: Ali
authors.initials: A
authors.lastname: Ceylan
authors.forename: Kenan
authors.initials: K
authors.lastname: Haspolat
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification*; D011019:Pneumonia, Mycoplasma / Q000453:epidemiology* / Q000635:transmission; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014421:Turkey / Q000453:epidemiology