title: Performance of an ultraviolet light-emitting diode-induced fluorescence detector in capillary electrophoresis.
abstract: The performance of a fluorescence detector in capillary electrophoresis (CE) using a light-emitting diode (LED) as excitation source is reported. An ultraviolet LED pulsed at a repetition rate of 500 Hz, combined with a time-discrimination and averaging acquisition system, was used. Limits of detection of 3 and 18 fmoles (at a signal-to-noise ratio equal to 3) were achieved for fluorescamine-derivatized bradykinin and lysine, respectively. This system exhibited a linear response for a concentration range between 54 and 417 microM for derivatized lysine, and between 1.81 and 23.58 microM for derivatized bradykinin. This detection system showed to be very convenient for routine analytical applications.
authors.affiliation: Centro de Biotecnologia do Estado do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Sandro
authors.initials: S
authors.lastname: Hillebrand
authors.forename: Júlio Ricardo
authors.initials: JR
authors.lastname: Schoffen
authors.forename: Marcos
authors.initials: M
authors.lastname: Mandaji
authors.forename: Carlos
authors.initials: C
authors.lastname: Termignoni
authors.forename: Hans Peter Henrik
authors.initials: HP
authors.lastname: Grieneisen
authors.forename: Tarso Benigno Ledur
authors.initials: TB
authors.lastname: Kist
mesh_terms: D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000032:analysis; D001920:Bradykinin / Q000031:analogs & derivatives / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D005450:Fluorescamine; D007202:Indicators and Reagents; D008239:Lysine / Q000031:analogs & derivatives / Q000032:analysis; D010447:Peptide Hydrolases / Q000032:analysis; D012694:Serine / Q000031:analogs & derivatives / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods* / Q000706:statistics & numerical data; D014466:Ultraviolet Rays
chemical_list: D007202:Indicators and Reagents; D001224:Aspartic Acid; D005450:Fluorescamine; D012694:Serine; D010447:Peptide Hydrolases; C035855:keratinase; D008239:Lysine; D001920:Bradykinin