Bioactive phenolic derivatives from Acaena splendens methanol extract.
Acaena splendens H. et A. has been used in Chilean folk medicine for the treatment of fever and inflammation. A description of the in vivo reduction of bacterial pyrogen-induced fever in rabbits and carrageenan-induced paw oedema in guinea pigs is presented. The methanol extract named ME-1, obtained after succesive extractions with petroleum ether and dichloromethane, showed a strong antipyretic action (45.7% of effect), though the antiinflammatory activity was only observed after submitting this extract to column fractionation, giving a crude mixture of flavonoids named C4 with both activities (55.7% and 98.9% of antiinflammatory and antipyretic effect respectively at a dose of 600 mg/kg). The bioassay-guided fractionation by column chromatography afforded the active fraction, which contained (-,-)-epicatechin, tiliroside, 7-O-acetyl-3-O-beta-D-glucosyl-kaempferol and 7-beta-D-glucosyloxy-5-hydroxy-chromone.
Backhouse
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Pharmacognosy Laboratory, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, P.O. Box 233, Santiago 1, Chile.
Delporte
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Negrete
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Feliciano
S A San
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López-Pérez
J L
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D018712:Analgesics, Non-Narcotic / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification; D002351:Carrageenan / Q000494:pharmacology; D002392:Catechin / Q000737:chemistry / Q000302:isolation & purification; D002867:Chromones / Q000737:chemistry / Q000302:isolation & purification; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005260:Female; D005334:Fever / Q000188:drug therapy; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D006168:Guinea Pigs; D006614:Hindlimb / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy; D044949:Kaempferols; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008519:Medicine, Traditional; D015394:Molecular Structure; D009288:Naproxen / Q000494:pharmacology; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D011817:Rabbits; D027824:Rosaceae
C468755:7-O-acetyl-3-O-glucosylkaempferol; C468756:7-glucosyloxy-5-hydroxychromone; D018712:Analgesics, Non-Narcotic; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001578:Benzopyrans; D002867:Chromones; D005419:Flavonoids; D005960:Glucosides; D044949:Kaempferols; D010636:Phenols; D010936:Plant Extracts; C052083:tiliroside; D009288:Naproxen; D002392:Catechin; D002351:Carrageenan