TACC3 expression and localization in the murine egg and ovary.
A protein spot cored from a silver-stained two dimensional (2D) gel of germinal vesicle stage immature mouse oocytes was identified as Transforming Acidic Coiled Coil containing protein (TACC3) by tandem mass spectrometry. PCR amplification revealed two alternatively spliced forms, Tacc3a and Tacc3b, in mouse ovarian cDNA libraries. TACC3a encoded a 630 aa protein with a predicted mass of 70 kDa. It contained seven 24 aa repeats at the N-terminus and two coiled-coil domains at the C-terminus. TACC3b encoded a 426 aa protein with a predicted mass of 49 kDa also containing two coiled coil domains, but lacking the 168 aa repeat region. In addition to homology to the TACC family members, murine TACC3 also showed 35.7% identity to the Xenopus protein, Maskin, a cytoplasmic polyadenylation element binding protein (CPEB)-associated factor. Northern blot analysis demonstrated that TACC3a is abundantly expressed in adult testis and spleen and is moderately expressed in the ovary, heart, and lung, suggesting a wide tissue distribution. Both myc-tagged TACC3a and TACC3b targeted to the cytoplasm of transiently transfected CV-1 cells. In situ hybridization of mouse ovarian tissue sections displayed abundant expression of TACC3 specifically in the cytoplasm of growing oocytes, but not in primordial or atretic follicles. This pattern of expression suggests that TACC3 is expressed in ovarian cells undergoing active growth and development.
Hao
Zhonglin
Z
Department of Cell Biology and Center for Recombinant Gamete Immunocontraceptive Vaccinogens, University of Virginia, Charlottesville, Virginia 22908, USA.
Stoler
Mark H
MH
Sen
Buer
B
Shore
Amy
A
Westbrook
Anne
A
Flickinger
Charles J
CJ
Herr
John C
JC
Coonrod
Scott A
SA
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D020033:Protein Isoforms; D020543:Proteome / Q000378:metabolism; D016415:Sequence Alignment; D013737:Testis / Q000378:metabolism
D008869:Microtubule-Associated Proteins; D020033:Protein Isoforms; D020543:Proteome; C120551:TACC3 protein, human