Inherited predisposition to CLL is detectable as subclinical monoclonal B-lymphocyte expansion.
Monoclonal chronic lymphocytic leukemia (CLL)-phenotype cells are detectable in 3.5% of otherwise healthy persons using flow cytometric analysis of CD5/CD20/CD79b expression on CD19-gated B cells. To determine whether detection of such CLL-phenotype cells is indicative of an inherited predisposition, we examined 59 healthy, first-degree relatives of patients from 21 families with CLL. CLL-phenotype cells were detected in 8 of 59 (13.5%) relatives, representing a highly significant increase in risk (P =.00002). CLL-phenotype cell levels were stable with time and had the characteristics of indolent CLL. Indolent and aggressive clinical forms were found in family members, suggesting that initiation and proliferation involves distinct factors. The detection of CLL-phenotype cells provides a surrogate marker of carrier status, potentially facilitating gene identification through mapping in families and direct analysis of isolated CLL-phenotype cells.
Rawstron
Andy C
AC
Academic Unit of Haematology and Oncology, University of Leeds, HMDS, West Yorkshire, Surrey, United Kingdom.
Yuille
Martin R
MR
Fuller
Julie
J
Cullen
Matthew
M
Kennedy
Ben
B
Richards
Stephen J
SJ
Jack
Andrew S
AS
Matutes
Estella
E
Catovsky
Daniel
D
Hillmen
Peter
P
Houlston
Richard S
RS
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000235:genetics*; D001402:B-Lymphocytes / Q000276:immunology*; D005190:Family; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D016130:Immunophenotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D015995:Prevalence
D015703:Antigens, CD