A bovine whole-genome radiation hybrid panel and outline map.
A 3000-rad radiation hybrid panel was constructed for cattle and used to build outline RH maps for all 29 autosomes and the X and Y chromosomes. These outline maps contain about 1200 markers, most of which are anonymous microsatellite loci. Comparisons between the RH chromosome maps, other published RH maps, and linkage maps allow regions of chromosomes that are poorly mapped or that have sparse marker coverage to be identified. In some cases, mapping ambiguities can be resolved. The RH maps presented here are the starting point for mapping additional loci, in particular genes and ESTs that will allow detailed comparative maps between cattle and other species to be constructed. Radiation hybrid cell panels allow high-density genetic maps to be constructed, with the advantage over linkage mapping that markers do not need to be polymorphic. A large quantity of DNA has been prepared from the cells forming the RH panel reported here and is publicly available for mapping large numbers of loci.
Williams
John L
JL
Roslin Institute (Edinburgh), Roslin, Midlothian, Scotland EH25 9PS, United Kingdom. John.Williams@BBSRC.AC.UK
Eggen
André
A
Ferretti
Luca
L
Farr
Christine J
CJ
Gautier
Mathieu
M
Amati
Giuseppe
G
Ball
Glynn
G
Caramorr
Tiziana
T
Critcher
Ricky
R
Costa
Sandro
S
Hextall
Patrick
P
Hills
David
D
Jeulin
Aurore
A
Kiguwa
Susanna L
SL
Ross
Olivia
O
Smith
Angela L
AL
Saunier
Katiana
K
Urquhart
Barbara
B
Waddington
Dave
D
D000818:Animals; D002417:Cattle / Q000235:genetics*; D005819:Genetic Markers; D016678:Genome; D008297:Male; D018895:Microsatellite Repeats; D021101:Radiation Hybrid Mapping
D005819:Genetic Markers