Construction of eukaryotic expression vector of thrombospondin-1 type I repeat sequence.
OBJECTIVE
To construct eukaryotic expression vector of thrombospondin-1 type I repeat sequences.


METHODS
Thrombospondin-1 type I repeat sequence gene was amplified from human fetal lung tissue by reverse transcriptase-PCR (RT-PCR) to construct both recombinant clone vector and expression vector through coupling reaction, followed by transforming these vectors into E.coli DH5alpha. The positive clones were selected for verification by double enzyme digestion and sequence analysis.


RESULTS
The expected amplification product, thrombospondin-1 type I repeat gene sequence, was acquired by RT-PCR, which had a consistency up to 99% with the sequence from the GenBank.


CONCLUSIONS
The eukaryotic expression vector PcDNA3.1(+)/TSP-1 type I repeat sequence was successfully constructed.
Li
Xiao-Wei
XW
Department of Cardiovascular Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Liu
Yi-Li
YL
Wu
Ping-Sheng
PS
Zhou
Zhong-Jiang
ZJ
D004587:Electrophoresis, Agar Gel; D005057:Eukaryotic Cells; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019700:Thrombospondin 1 / Q000235:genetics*
D019700:Thrombospondin 1