Distribution, immunohistochemical characteristics and nerve pathways of primary sensory neurons supplying the porcine vas deferens.
The present study investigated: (1) the distribution and chemical coding of primary sensory neurons supplying the vas deferens in juvenile pigs by the use of retrograde tracing combined with double-labelling immunofluorescence, (2) nerve pathways from dorsal root ganglia (DRG) to the vas deferens by means of denervation procedures involving transection of the hypogastric or pelvic nerve combined with a retrograde tracing method, and (3) possible interactions of the substance P (SP)/calcitonin gene-related peptide (CGRP)-immunoreactive varicose nerve fibres on vas deferens projecting neurons (VDPN) in the anterior pelvic ganglion (APG). The vast majority of VDPN were found mainly in the lumbar L2, L3 and sacral S2, S3 pairs of DRG and showed a clear ipsilaterally organized projection pattern. Immunohistochemistry revealed that most of these neurons contained SP and/or CGRP, occasionally coexpressed with galanin. Interestingly, pronounced differences in the expression of SP and/or CGRP were observed between the lumbar and sacral VDPN in that most of the lumbar but less than half of the sacral neurons stained for these peptides. Denervation experiments showed that the neurons located within the lumbar DRG project through the ipsilateral hypogastric nerve, whereas those found within the sacral DRG send their processes through the ipsilateral and contralateral pelvic nerve. In the nerve-lesioned animals, especially in those with the hypogastric nerve cut, a dramatic reduction in the number of SP and/or CGRP-containing nerve terminals surrounding the efferent VDPN within the APG was observed. This study has disclosed the distribution and, for the first time, chemical coding and nerve pathways of vas deferens-projecting primary sensory neurons in a mammalian species, the pig. The results obtained also provide some novel information about the possible morphological and functional relationship between vas deferens-projecting primary sensory and pelvic efferent nerve cells.
Kaleczyc
Jerzy
J
Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn-Kortowo II, Bldg. 105J, Poland. jerzyk@moskit.uwm.edu.pl
Scheuermann
Dietrich W
DW
Pidsudko
Zenon
Z
Majewski
Mariusz
M
Lakomy
Miroslaw
M
Timmermans
Jean-Pierre
JP
D000344:Afferent Pathways / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D004525:Efferent Pathways / Q000166:cytology / Q000378:metabolism; D005725:Ganglia, Autonomic / Q000166:cytology / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology* / Q000378:metabolism; D007001:Hypogastric Plexus / Q000166:cytology* / Q000378:metabolism; D007150:Immunohistochemistry; D008159:Lumbar Vertebrae; D008297:Male; D009475:Neurons, Afferent / Q000166:cytology* / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D012447:Sacrum; D011984:Sensory Receptor Cells / Q000166:cytology* / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D034421:Sus scrofa / Q000033:anatomy & histology / Q000378:metabolism*; D014649:Vas Deferens / Q000294:innervation*
D013373:Substance P; D015740:Calcitonin Gene-Related Peptide