title: Characterization of a general amino acid permease from Hebeloma cylindrosporum.
abstract: Besides a role in phosphate supply, ectomycorrhizas play a crucial role in nitrogen nutrition of plants. The ectomycorrhizal association between Hebeloma cylindrosporum and Pinus pinaster serves as a model system accessible to molecular manipulation. Hebeloma mycelium is able to take up and use amino acids as the sole nitrogen source. Suppression cloning allowed identification of a Hebeloma transporter (HcGAP1) mediating histidine uptake. HcGAP1 mediates secondary active uptake of a wide spectrum of different amino acids. The secondary active transport mechanism together with the expression in hyphae, but not in mycorrhizas, indicate a role in uptake of organic nitrogen from the soil.
authors.affiliation: ZMBP, Plant Physiology, Auf der Morgenstelle 1, D-72076 Tübingen, Germany. daniel.wipf@zmbp.uni-tuebingen.de
authors.forename: Daniel
authors.initials: D
authors.lastname: Wipf
authors.forename: Mariam
authors.initials: M
authors.lastname: Benjdia
authors.forename: Mechthild
authors.initials: M
authors.lastname: Tegeder
authors.forename: Wolf B
authors.initials: WB
authors.lastname: Frommer
mesh_terms: D000363:Agaricales / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D026905:Amino Acid Transport Systems / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D001693:Biological Transport, Active; D003001:Cloning, Molecular; D005800:Genes, Fungal; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D013559:Symbiosis; D014197:Trees / Q000378:metabolism / Q000382:microbiology
chemical_list: D026905:Amino Acid Transport Systems; D000596:Amino Acids; D001224:Aspartic Acid; D009584:Nitrogen