title: [Influence of the stratum corneum and dermis of the skin on the percutaneous absorption of fluorouracil delivered through skin patch].
abstract: OBJECTIVE
  To investigate the respective influence of skin layers, specifically the stratum corneum (SC) and the viable layer, and isopropyl myristate (IPM), an absorption enhancer, on the permeability (K(p)) of drug.
  
  
  METHODS
  Patches saturated with fluorouracil (5-FU), which was used as a test drug, were prepared. The Franz diffusion experiment via different rat skin layers was carried out, and high-performance liquid chromatography (HPLC) or gas chromatography (GC) was employed to determine the contents of 5-FU and IPM. K(p) of 5-FU in various conditions was calculated.
  
  
  RESULTS
  The K(p) of 5-FU via SC -stripped skin was 2.3 times that via intact skin, and in the presence of IPM, K(p) was 1.8 times that via intact skin and 2.4 times that via SC-stripped skin.
  
  
  CONCLUSIONS
  The permeability of 5-FU is enhanced after the SC is stripped, and IPM can overcome the barrier of SC in intact skin to facilitate 5-FU absorption, but can not enhance the K(p) of 5-FU via SC-stripped skin.
authors.affiliation: Department of Pharmacy, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. lgfnf@fimmu.com
authors.forename: Guo-feng
authors.initials: GF
authors.lastname: Li
authors.forename: Jian-hai
authors.initials: JH
authors.lastname: Chen
authors.forename: Fumio
authors.initials: F
authors.lastname: Kamiyama
authors.forename: Akira
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Zhi-liang
authors.initials: ZL
authors.lastname: Chen
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D020405:Dermis / Q000378:metabolism*; D005472:Fluorouracil / Q000008:administration & dosage / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D051381:Rats; D017208:Rats, Wistar; D012869:Skin Absorption
chemical_list: D000964:Antimetabolites, Antineoplastic; D005472:Fluorouracil