title: Co-expression of interleukin-1beta and tumor necrosis factor alpha in synovial tissues and synovial fluids of temporomandibular joint with internal derangement: comparison with histological grading of synovial inflammation.
abstract: BACKGROUND
  It has been clarified that interleukin-1 (IL-1)beta and tumor necrosis factor (TNF)alpha play an important role in pathogenesis of various joint disease. The purpose of this study was to investigate the cellular source of IL-1beta and TNFalpha in temporomandibular joint (TMJ), and to analyze the relation between the expression of these cytokines and the intensity of TMJ synovial inflammation.
  
  
  METHODS
  We examined 33 synovial biopsy specimens from patients with internal derangement of the TMJ by an immunohistochemical technique using specific antibodies to IL-1beta and TNFalpha. We also studied 20 synovial fluids from the patients by enzyme-linked immunosorbent assay method. These data are compared with histological grading of synovial inflammation by Gynther's system.
  
  
  RESULTS
  Both IL-1beta and TNFalpha were predominantly localized in the synovial lining cell layer and the blood vessels of synovial biopsy specimens obtained from patients with TMJ internal derangement. A statistically significant correlation was found between the intensity of IL-1beta expression and that of TNFalpha. Additionally, the intensity of TNFalpha expression was statistically correlated with histological grading by Gynther's system.
  
  
  CONCLUSIONS
  These results supported that IL-1beta and TNFalpha may be involved in the occurrence of TMJ internal derangement and that they coordinately play an role in pathogenesis of TMJ internal derangement.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kanazawa Medical University, Ishikawa, Japan. suzukig3@kanazawa-med.ac.jp
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Suzuki
authors.forename: Natsuki
authors.initials: N
authors.lastname: Segami
authors.forename: Masaaki
authors.initials: M
authors.lastname: Nishimura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nojima
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology; D004204:Joint Dislocations / Q000378:metabolism; D008297:Male; D018709:Statistics, Nonparametric; D013582:Synovial Fluid / Q000378:metabolism; D013583:Synovial Membrane / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D013585:Synovitis / Q000378:metabolism* / Q000473:pathology; D013705:Temporomandibular Joint Disorders / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology
chemical_list: D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha