[Use of colloid gold conjugates for identification of actins of various origin].
Some optimized methods of purifying actin from animal, plant, and bacterial cells were developed. Variants of the preparation of antiactin antibodies are described which use both traditional methods of immunization and phase display technology and antigen adsorption on colloidal gold particles. The conjugates of colloidal gold with phalloidin and heavy meromyosin as well as with antibodies were proposed. It was shown that these markers make it possible to reliably identify actins of different origin by the methods of light, and electron microscopy and dot-analysis.
Dykman
L A
LA
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015 Russia.
Bogatyrev
V A
VA
Zaĭtseva
I S
IS
Sokolova
M K
MK
Ivanov
V V
VV
Sokolov
O I
OI
D000199:Actins / Q000737:chemistry* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D015806:Azospirillum brasilense / Q000737:chemistry / Q000648:ultrastructure; D015415:Biomarkers / Q000737:chemistry; D002645:Chickens; D017979:Gold Colloid / Q000737:chemistry* / Q000276:immunology; D018551:Solanum lycopersicum / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D015879:Myosin Subfragments / Q000737:chemistry / Q000276:immunology; D010590:Phalloidine / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D014908:Triticum / Q000737:chemistry
D000199:Actins; D000906:Antibodies; D015415:Biomarkers; D017979:Gold Colloid; D015879:Myosin Subfragments; D010590:Phalloidine