Identification of the Tslc1 gene, a mouse orthologue of the human tumor suppressor TSLC1 gene.
We have recently identified the TSLC1 gene as a novel tumor suppressor in human non-small lung cancer on chromosome 11q23.2. TSLC1 encodes a membrane glycoprotein showing significant homology with immunoglobulin superfamily molecules. Here, we report the isolation of a mouse orthologous gene, Tslc1. The Tslc1 cDNA contains a single open reading frame of 1335 bp encoding a putative protein of 445 amino acids, and its expression was detected in all tissues examined. The Tslc1 gene is mapped on mouse chromosome 9, a synteny of human chromosome 11q, and is composed of ten exons, the exon-intron junctions being highly conserved between human and mouse. The predicted amino acids of mouse Tslc1 display 98% identity with that of human TSLC1. Furthermore, data base analysis indicates that the amino acid sequences corresponding to the cytoplasmic domain of Tslc1 are identical in five mammals and highly conserved in vertebrates, suggesting an important role of Tslc1 in normal cell-cell interaction.
Fukami
Takeshi
T
Tumor Suppression & Functional Genomics Project, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan.
Satoh
Hitoshi
H
Fujita
Eriko
E
Maruyama
Tomoko
T
Fukuhara
Hiroshi
H
Kuramochi
Masami
M
Takamoto
Shinichi
S
Momoi
Takashi
T
Murakami
Yoshinori
Y
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005091:Exons; D015870:Gene Expression; D005796:Genes / Q000235:genetics; D016147:Genes, Tumor Suppressor; D006801:Humans; D007136:Immunoglobulins; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D026801:Synteny; D025521:Tumor Suppressor Proteins
C403514:CADM1 protein, human; C498598:Cadm1 protein, mouse; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D007136:Immunoglobulins; D008565:Membrane Proteins; D011506:Proteins; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins