Increased expression of human luteinizing hormone/human chorionic gonadotropin receptor mRNA in human endometrial cancer.
Fifty randomly selected endometrial tissue samples were used in this study to determine the existence of LH/hCG receptor. Of these, 6 were matched samples of cancerous and surrounding morphologically normal tissues from the same individuals. Conventional RT-PCR, nested RT-PCR, and relative real-time PCR (TaqMan) methods were used to measure the gene expression on the RNA levels. The results show, (1) Real-time PCR can detect LH/hCG receptor expression in all the RNA samples analyzed when conventional RT-PCR only demonstrates the expression of LH/hCG receptor in 17% of the tested samples. (2) Absolute quantitation of LH/hCG receptor by real-time PCR shows higher expression in cancer tissue. This finding was confirmed by the six matched samples. It suggests that LH/hCG receptor gene expresses in endometrium at very low levels and real-time PCR is the best method to detect the expression. Quantitative real-time PCR also reveals increased expressions of LH/hCG receptor in endometrial cancerous tissues compared with surrounding microscopically normal tissues.
Ji
Qing
Q
Department of Pathology, Olive View-UCLA Medical Center, Sylmar, California 91342-1495, USA.
Chen
Peter
P
Aoyoma
Chisa
C
Liu
Paul
P
D017931:DNA Primers; D016889:Endometrial Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics; D011974:Receptors, LH / Q000235:genetics*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011974:Receptors, LH