Time dependent responses of glycolytic intermediates in a detailed glycolytic model of Lactococcus lactis during glucose run-out experiments.
Glucose addition and subsequent run-out experiments were compared to simulations with a detailed glycolytic model of Lactococcus lactis. The model was constructed largely on bases of enzyme kinetic data taken from literature and not adjusted for the specific simulations shown here. Upon glucose depletion a rapid increase in PEP, inorganic phosphate and a gradual decrease in fructose 1,6-bisphosphate (FBP) were measured and predicted by simulation. The dynamic changes in these and other intermediate concentrations as measured in the experiments were well predicted by the kinetic model.
Hoefnagel
M H N
MH
Wageningen Centre for Food Sciences, Food and Bioprocess Engineering Group, Wageningen University, The Netherlands. marcel.hoefnagel@algemeen.pk.wau.nl
van der Burgt
A
A
Martens
D E
DE
Hugenholtz
J
J
Snoep
J L
JL
D000255:Adenosine Triphosphate / Q000378:metabolism; D003198:Computer Simulation; D005635:Fructosediphosphates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D007700:Kinetics; D013294:Lactococcus lactis / Q000378:metabolism*; D008954:Models, Biological; D009243:NAD / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D010728:Phosphoenolpyruvate / Q000378:metabolism
D005635:Fructosediphosphates; D009243:NAD; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate; D005947:Glucose; C029063:fructose-1,6-diphosphate