Preformulation study of the vaccine candidate TAB9 against HIV-1.
A preformulation study was performed for the evaluation of a vaccine candidate against HIV-1. Aluminium hydroxide was used in the preformulation. However, this adjuvant is not a good adsorbent for basic proteins since it is positively charged at a physiological pH. In the present study, we determined the adsorption of TAB9 (basic protein, pI: 11.3) by treating Alhydrogel with different ions. The immunogenicity of the vaccine candidate against HIV was also evaluated using three batches, 9801-A, 9802-A and 9803-A, and a placebo P-001. The evaluation was performed twice (0 and 9 months). Each batch was tested using groups of 10 mice that had a single inoculation. The results showed that the protein was totally adsorbed to the aluminium gel. Seroconvertion was attained in all analysed batches, indicating the potentiality of TAB9 as a vaccine candidate.
Expósito Raya
Néstor
N
Departamento de Desarrollo de Formulaciones, Centro de Ingenieri;a Genética y Biotecnologi;a, P.O. Box 6162, Cubanacán, Havana 10600, Cuba.
García Díaz
Alina
A
Carrazana López
Yamilka
Y
Quintana Vazquez
Diógenes
D
Pichardo Díaz
Dagmara
D
Martínez de la Puente
Nieves
N
Duarte Cano
Carlos
C
D016915:AIDS Vaccines / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000737:chemistry; D000327:Adsorption; D000536:Aluminum Hydroxide / Q000737:chemistry*; D000818:Animals; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology*; D005260:Female; D015488:HIV Antigens / Q000276:immunology; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010710:Phosphates / Q000737:chemistry; D012016:Reference Values; D012680:Sensitivity and Specificity; D014614:Vaccines, Synthetic / Q000276:immunology*
D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D000939:Epitopes; D015488:HIV Antigens; D010710:Phosphates; C417682:TAB9; D014614:Vaccines, Synthetic; D000536:Aluminum Hydroxide