title: Diagnosis of group A coxsackieviral infection using polymerase chain reaction.
abstract: OBJECTIVE
  To examine the relation between enteroviral infection, especially group A coxsackieviral infection, and acute febrile illness over two summers using tissue culture and polymerase chain reaction (PCR).
  
  
  METHODS
  Throat swabs were collected from 246 children from June to August 1997 and 1998.
  
  
  RESULTS
  Enteroviruses were isolated from 33/246 samples and 35 other viruses were isolated. Enteroviral genomes were detected in 54/178 samples from which no virus was isolated. Of 41 enteroviral genotypes identified by sequence analysis of PCR products, 38 were group A coxsackieviruses, which are usually difficult to isolate using tissue culture.
  
  
  CONCLUSIONS
  Results indicate that viral detection and identification based on PCR is useful in the diagnosis of group A coxsackieviral infection.
authors.affiliation: Department of Pediatrics, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan. mhosoya@fmu.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Hosoya
authors.forename: H
authors.initials: H
authors.lastname: Ishiko
authors.forename: Y
authors.initials: Y
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Honzumi
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000208:Acute Disease; D002648:Child; D002675:Child, Preschool; D046508:Culture Techniques; D029821:Enterovirus A, Human / Q000145:classification / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis*; D005334:Fever / Q000821:virology; D016679:Genome, Viral; D006557:Herpangina / Q000821:virology; D006801:Humans; D007223:Infant; D010612:Pharyngitis / Q000821:virology; D010614:Pharynx / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D003294:Seizures, Febrile / Q000821:virology; D014069:Tonsillitis / Q000821:virology; D014773:Virology / Q000379:methods