title: Comparative positions of kinetoplasts in Trypanosoma musculi and Trypanosoma lewisi during development in vitro.
abstract: The development of Trypanosoma musculi and Trypanosoma lewisi were studied in vitro in the presence of adherent splenic cells. Both parasites developed only when attached by their flagellar tips to adherent splenic cells. During the proliferation of T. musculi, the kinetoplast migrated towards the nucleus, and once in the vicinity of the nucleus, the nuclear division was triggered. The kinetoplast of T. lewisi did not migrate towards the nucleus, but remained at its original location. The nucleus and kinetoplast divided at the same time in both parasites, and parasites started dividing from their flagellar ends and T. musculi and T. lewisi daughter cells were formed within 48 h. The unavailability of the adherent splenic cells in vitro led the parasites to transform into round/oval nonviable forms.
authors.affiliation: Department of Biology, Howard University, Washington, DC 20059, USA. mashraf@howard.edu
authors.forename: M
authors.initials: M
authors.lastname: Ashraf
authors.forename: R A
authors.initials: RA
authors.lastname: Nesbitt
authors.forename: P A
authors.initials: PA
authors.lastname: Humphrey
authors.forename: M
authors.initials: M
authors.lastname: Siewe
authors.forename: C M
authors.initials: CM
authors.lastname: Lee
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D015388:Organelles / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000469:parasitology; D014345:Trypanosoma / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure*; D014350:Trypanosoma lewisi / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure*