title: Effect of topical iganidipine on experimental elevation of aqueous flare induced by prostaglandin E(2) and EP agonists in pigmented rabbits.
abstract: We evaluated the role of topical iganidipine on experimental aqueous flare elevation in rabbits. Transcorneal diffusion of prostaglandin E(2) (PGE(2)), 25 microg/ml or 7.09 x 10(-2) mmol/l, or highly selective agonists for prostaglandin E(2) receptor subtypes (EP), 25 microg/ml, was achieved with the use of a glass cylinder to produce aqueous flare elevation in pigmented rabbits. Iganidipine was topically administered before application of PGE(2) or EP agonists. Aqueous flare was measured with a laser flare cell meter. Topical instillation of 0.1% iganidipine once or twice inhibited 64 +/- 8% (p < 0.01) and 84 +/- 9% (p < 0.01) of PGE(2)-induced aqueous flare elevation, respectively. Two instillations of 0.1% iganidipine inhibited 95 +/- 5% (p < 0.01) of EP2-agonist(ONO-AE1-259-01)-induced flare elevation and 98 +/- 3% (p < 0.01) of EP4-agonist(ONO-AE1-392)-induced flare rise. Topical iganidipine may have anti-inflammatory activity in the eye.
authors.affiliation: Department of Ophthalmology, Toyama Medical and Pharmaceutical University, Toyama, Japan. ophthal@ms.toyama-mpu.ac.jp
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Yanagisawa
authors.forename: Seiji
authors.initials: S
authors.lastname: Hayasaka
authors.forename: Xue-Yun
authors.initials: XY
authors.lastname: Zhang
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Hayasaka
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Nagaki
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001082:Aqueous Humor / Q000187:drug effects*; D002121:Calcium Channel Blockers / Q000008:administration & dosage* / Q000494:pharmacology; D015232:Dinoprostone / Q000819:agonists*; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology; D010879:Piperazines / Q000008:administration & dosage* / Q000494:pharmacology; D020033:Protein Isoforms / Q000819:agonists; D011725:Pyridines / Q000008:administration & dosage* / Q000494:pharmacology; D011817:Rabbits; D018078:Receptors, Prostaglandin E / Q000819:agonists*
chemical_list: D002121:Calcium Channel Blockers; D008738:Methyl Ethers; C461399:ONO-AE1-329; D010879:Piperazines; D020033:Protein Isoforms; D011725:Pyridines; D018078:Receptors, Prostaglandin E; C108540:iganidipine; D015232:Dinoprostone