Myoglobin and cytochrome b5: a nuclear magnetic resonance study of a highly dynamic protein complex.
The transient complex of bovine myoglobin and cytochrome b(5) has been investigated using a combination of NMR chemical shift mapping, (15)N relaxation data, and protein docking simulations. Chemical shift perturbations observed for cytochrome b(5) amide resonances upon complex formation with either metmyoglobin (Fe(III)) or carbon monoxide-bound myoglobin (Fe(II)) are more than 10-fold smaller than in other transient redox protein complexes. From (15)N relaxation experiments, an increase in the overall correlation time of cytochrome b(5) in the presence of myoglobin is observed, confirming that complex formation is occurring. The chemical shift perturbations of proton and nitrogen amide nuclei as well as heme protons of cytochrome b(5) titrate with increasing myoglobin concentrations, also demonstrating the formation of a weak complex with a K(a) in the inverse millimolar range. The perturbed residues map over a wide surface area of cytochrome b(5), with patches of residues located around the exposed heme 6-propionate as well as at the back of the protein. The nature of the affected residues is mostly negatively charged contrary to perturbed residues in other transient complexes, which are mainly hydrophobic or polar. Protein docking simulations using the NMR data as constraints show several docking geometries both close to and far away from the exposed heme propionates of myoglobin. Overall, the data support the emerging view that this complex consists of a dynamic ensemble of orientations in which each protein constantly diffuses over the surface of the other. The characteristic NMR features may serve as a structural tool for the identification of such dynamic complexes.
Worrall
Jonathan A R
JA
Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Liu
Yijeng
Y
Crowley
Peter B
PB
Nocek
Judith M
JM
Hoffman
Brian M
BM
Ubbink
Marcellus
M
D000818:Animals; D001665:Binding Sites; D001704:Biopolymers / Q000737:chemistry; D002417:Cattle; D015786:Cytochromes b5 / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry; D008786:Metmyoglobin / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011522:Protons; D013816:Thermodynamics
D001704:Biopolymers; D005290:Ferric Compounds; D009211:Myoglobin; D009587:Nitrogen Isotopes; D011522:Protons; C018013:carboxymyoglobin; D008786:Metmyoglobin; D015786:Cytochromes b5