The expression of p73, p21 and MDM2 proteins in gliomas.
p73 protein, a member of the p53 family protein, induce p21 and MDM2 transcription. In some carcinomas, the expression of p73 was higher in carcinoma than in normal tissue, and the overexpression was correlated to grade, stage or prognosis. However, either the expression of p73 protein or the relationship among p73, p21 and MDM2 proteins was not known well in glioma. In this study, we examined the expression of p73, p21 and MDM2 proteins immunohistochemically and analyzed the relationship among these three proteins in sixty surgical specimens of gliomas including 10 glioblastomas, 10 anaplastic astrocytomas, 6 diffuse astrocytomas, 8 pilocytic astrocytomas, 1 anaplastic ependymoma, 8 ependymomas, 9 anaplastic oligodendroglial tumors and 8 low grade oligodendroglial tumors. The p73 labelling index (LI)s and p21 LIs differed among the tumor types, but there was no difference in the MDM2 LIs among all of the tumor types. The mean p73 LI of ependymomas was significantly higher than in any other tumor type. The mean p2I LIs of ependymomas and pilocytic astrocytomas were significantly higher than in any other tumor type. There were no significant correlations among p73 LIs, p21 LIs, MDM2 LIs and MIB-1 LIs in all p73 immunopositive tumors. The present results suggest that p73 and p21 overexpression of ependymomas and p21 overexpression of pilocytic astrocytomas are one of the features of these tumors, and that p73 overexpression does not influence the expression manners of either p21 or MDM2 in gliomas.
Kamiya
Makoto
M
Department of Pathology, Gunma University School of Medicine, Maebashi, Japan. mkamiya@med.gunma-u.ac.jp
Nakazato
Yoichi
Y
D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D016147:Genes, Tumor Suppressor; D005910:Glioma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D009687:Nuclear Proteins / Q000096:biosynthesis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D051736:Proto-Oncogene Proteins c-mdm2; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis; D013347:Subcellular Fractions; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; D015854:Up-Regulation
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)