Production of bacteriocins from Lactococcus lactis subsp. lactis CECT 539 and Pediococcus acidilactici NRRL B-5627 using mussel-processing wastes.
The growth and bacteriocin production by Lactococcus lactis subsp. lactis CECT 539 and Pediococcus acidilactici NRRL B-5627 were investigated on mussel-processing wastes. Both bacteriocin productions were satisfactorily modelled using a modified form of the Luedeking and Piret expression, which includes a term for the influence of the pH reduction rate. Experimental data from cultures buffered at different initial concentrations (0, 0.03, 0.10 and 0.25 M) of both bacteria were used to fit and verify the model. The influence of total sugars, nitrogen, phosphorus and buffer concentration on nisin and pediocin production was also studied using response-surface methodology and empirical modelling. Enhanced nisin production (33 BU/ml) was achieved in media buffered with 0.10 M potassium hydrogen phthalate/NaOH. However, the highest levels of pediocin (368 BU/ml) were obtained in the non-buffered media.
Pérez Guerra
Nelson
N
Departamento de Bioquímica, Xenética e Inmunoloxía, Facultade de Ciencias de Ourense, Universidade de Vigo, As Lagoas, 32004 Ourense, Spain.
Pastrana Castro
Lorenzo
L
D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis*; D049872:Bivalvia; D002460:Cell Line; D003198:Computer Simulation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods; D007220:Industrial Waste; D013294:Lactococcus lactis / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D008954:Models, Biological; D009561:Nisin / Q000096:biosynthesis; D010376:Pediococcus / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D012037:Refuse Disposal / Q000379:methods; D012680:Sensitivity and Specificity; D013045:Species Specificity
D001430:Bacteriocins; D007220:Industrial Waste; D009561:Nisin