Detection of stem cell factor mRNA expression in leukemic cells by in situ reverse transcriptase-PCR.
OBJECTIVE
To evaluate the feasibility of detecting low copy stem cell factor (SCF) mRNA in leukemic cells by in situ reverse transcriptase-PCR (RT-PCR).


METHODS
In situ RT-PCR was carried out on PE480 DNA Thermal Cycler to determine the expression of SCF mRNA in K562, HL-60 and chronic myelogenous leukemia (CML) cells in blast phase.


RESULTS
SCF mRNA was detected in the 3 cell lines by direct or indirect in situ RT-PCR, but not by in situ hybridization.


CONCLUSIONS
SCF mRNA is present in K562, HL-60 and CML cells in blast phase but with very low expression level which can be detected by in situ RT-PCR carried out on PE480 DNA Thermal Cycler.
Wu
Bin
B
Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. wubin63@yahoo.com.cn
Liu
Xiao-Li
XL
Xuan
Wen-Lan
WL
Feng
Ru
R
Yin
Fang
F
Zhou
Shu-Yun
SY
D016475:3T3 Cells; D000818:Animals; D015870:Gene Expression; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D051379:Mice; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019089:Stem Cell Factor / Q000235:genetics*; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D019089:Stem Cell Factor