Functional analysis of the tissue-engineered stomach wall.
We have established a method for in situ tissue engineering of the stomach in a canine model using an acellular collagen scaffold graft. The current study was conducted to evaluate the functional aspects of the tissue-engineered stomach wall. The anterior wall of the stomach in beagle dogs was replaced with a collagen sponge scaffold measuring 4 x 4 cm. At 16 weeks after implantation, the animals were sacrificed and the stomach specimens were evaluated immunohistochemically and physiologically. Regeneration of the proton pump and thin muscle layer, which are essential for mechanical and chemical digestion by the stomach, was observed in the tissue-engineered gastric tissue. However, acetylcholine-induced contraction was not observed in the tissue-engineered stomach wall. Although there is still room for improvement, the tissue-engineered stomach wall had a highly organized structure, and it is anticipated that this approach could eventually become an alternative for stomach reconstruction after gastrectomy.
Hori
Yoshio
Y
Department of Bioartificial Organs, Institute for Frontier Medical Science, Kyoto University, Japan. hori@frontier.kyoto-u.ac.jp
Nakamura
Tatsuo
T
Kimura
Dai
D
Kaino
Kenji
K
Kurokawa
Yoshimochi
Y
Satomi
Susumu
S
Shimizu
Yasuhiko
Y
D000818:Animals; D003094:Collagen / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004285:Dogs; D005069:Evaluation Studies as Topic; D005260:Female; D005743:Gastrectomy / Q000009:adverse effects*; D009119:Muscle Contraction / Q000502:physiology; D017494:Proton Pumps / Q000502:physiology; D020127:Recovery of Function / Q000502:physiology*; D013270:Stomach / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology*; D023822:Tissue Engineering
D017494:Proton Pumps; D003094:Collagen