Modulation of metabolite concentrations with no net effect on fluxes.
The concentration of a metabolite in a metabolic system can be varied without affecting any other concentrations or any fluxes by varying the concentrations of two inhibitors, one a competitive inhibitor of the enzyme that produces the metabolite, the other a competitive inhibitor of the enzyme that consumes it. The two concentrations need to be varied in opposite directions in such a way that the they add up to 100% when each is expressed as a percentage of the concentration that gives the desired flux in the absence of the other. The general approach can be extended to systems in which the inhibited enzymes do not catalyse consecutive reactions.
María
Luz Cárdenas
LC
IBSM-BIP-CNRS, Marseille, France. cardenas@ibsm.cnrs-mrs.fr
Ortega
Fernando
F
Cascante
Marta
M
Cornish-Bowden
Athel
A
D004791:Enzyme Inhibitors / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D008433:Mathematics; D008954:Models, Biological; D009097:Multienzyme Complexes
D004791:Enzyme Inhibitors; D004798:Enzymes; D009097:Multienzyme Complexes