title: Intragenomic length variation of the ribosomal DNA intergenic spacer in a malaria vector, Anopheles sinensis.
abstract: We determined the complete sequences of six size variants of intergenic spacer (IGS) region from one individual of the malaria vector mosquito species, Anopheles sinensis. All six size variants observed in this study show almost the same basic primary structure in which three repeat regions (A, B, and C) are interspersed by highly conserved nonrepeating sections. In contrast to the well-ordered subrepeating patterns found in A and C, the repeat region B displays extremely variable and complicated profiles in the number and arrangement of subrepeat units among different size classes. It is apparent that the prominent level of length difference in the repeat regions B and C is responsible for the intragenomic length variations of the IGS molecule observed in the present study. High level of sequence homology and regularly arranged repeating pattern of 11 to 14 bp motif sequences harbored within the B repeat region allow us to consider that these motif sequences may be associated with their potential role as a recombination site. Compared to those previously published in other mosquito species, the IGS of A. sinensis showed a very unique structural format in subrepeat patterns of the IGS region. This result suggests that the structure and sequence profiles of the IGS region would provide useful information for the exploitation of a convenient molecular marker to identify morphologically complicated species complex and to characterize the genetic variation of population. This suggestion is far from being conclusive at present, but a further genetic study will bring more compelling evidences for this pending issue.
authors.affiliation: School of Biological Sciences, Seoul National University, Korea.
authors.forename: In-Jung
authors.initials: IJ
authors.lastname: Whang
authors.forename: Jongwoo
authors.initials: J
authors.lastname: Jung
authors.forename: Joong-Ki
authors.initials: JK
authors.lastname: Park
authors.forename: Gi-Sik
authors.initials: GS
authors.lastname: Min
authors.forename: Won
authors.initials: W
authors.lastname: Kim
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D017931:DNA Primers; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers; D014644:Genetic Variation; D016678:Genome; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers