title: Regeneration of flowering plants from difficile lily protoplasts by means of a nurse culture.
abstract: The regeneration of difficile lily protoplasts isolated from suspension cells of the Oriental hybrid lily ( Lilium L.) cultivars Casablanca, Siberia and Acapulco was achieved by using the nurse-culture method. The divided protoplasts grew into colonies with nurse cells that have no regeneration ability, and developed to visible calli on a medium containing picloram. Many plantlets were formed on the calli after transfer of the proliferated calli to hormone-free medium. We were able to transplant the plantlets to soil in pots without acclimatization, and the plantlets grew in a greenhouse until flowering 2 years later.
authors.affiliation: Hokkaido Green-Bio Institute, Naganuma, Japan.
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Horita
authors.forename: Hitomi
authors.initials: H
authors.lastname: Morohashi
authors.forename: Fuminori
authors.initials: F
authors.lastname: Komai
mesh_terms: D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods; D035264:Flowers / Q000187:drug effects / Q000254:growth & development*; D006540:Herbicides / Q000494:pharmacology; D027762:Lilium / Q000166:cytology / Q000254:growth & development*; D018519:Meristem / Q000187:drug effects / Q000254:growth & development; D010846:Picloram / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D011523:Protoplasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
chemical_list: D003470:Culture Media; D006540:Herbicides; D010846:Picloram