Effect of tranilast on proliferation, collagen gel contraction, and transforming growth factor beta secretion of retinal pigment epithelial cells and fibroblasts.
The efficiency of tranilast for the treatment of proliferative vitreoretinopathy (PVR) was investigated in vitro. A tetrazolium-based colorimetric assay showed that the 300-microM concentration of tranilast inhibited proliferation of bovine retinal pigment epithelial (RPE) cells and rabbit dermal fibroblasts with no toxicity. The contraction of collagen gels embedded with these cells was evaluated in the cultures. Compared with the gel incubated with minimal essential medium and 0.35% bovine serum albumin and/or fetal calf serum, tranilast inhibited gel contraction. Enzyme-linked immunosorbent assay revealed that a 300-microM concentration of tranilast inhibited transforming growth factor-beta(1) (TGF-beta(1)) secretion significantly (p < 0.01). These results suggest that tranilast may inhibit the proliferation of RPE cells and fibroblasts and contraction of intraocular fibrous membranes by suppressing TGF-beta(1) secretion from these cells with a potential to treat PVR.
Yasukawa
Tsutoma
T
University Eye Hospital Leipzig, Germany.
Kimura
Hideya
H
Dong
Jin
J
Tabata
Yasuhiko
Y
Miyamoto
Hideki
H
Honda
Yoshihito
Y
Ogura
Yuichiro
Y
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D005782:Gels; D006634:Histamine H1 Antagonists / Q000008:administration & dosage / Q000494:pharmacology*; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000378:metabolism*; D011817:Rabbits; D012867:Skin / Q000166:cytology; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors*; D062367:ortho-Aminobenzoates / Q000008:administration & dosage / Q000494:pharmacology*
D005782:Gels; D006634:Histamine H1 Antagonists; D016212:Transforming Growth Factor beta; D062367:ortho-Aminobenzoates; D003094:Collagen; C012293:tranilast