title: Progenitors in the postnatal cerebellar white matter are antigenically heterogeneous.
abstract: Progenitors that migrate through the white matter of the postnatal cerebellum give rise to cortical interneurons, astroglia, and oligodendroglia. To determine whether this progenitor population is heterogeneous with respect to specific lineage markers, we infected progenitors in vivo with a retrovirus encoding the green fluorescent protein on postnatal day 4/5 and labeled them in situ with various antibodies 2 days postviral injection: the neuronal marker was the transcription factor SOX1; early oligodendroglial markers were chondroitin sulfate proteoglycan antigen and platelet-derived growth factor receptor-alpha. Markers for astroglial progenitors were vimentin, nestin, zebrin II, and the astroglial-specific glutamate transporter subtype GLAST. None of the progenitors was doubly labeled with any combination of markers characteristic for different cell lineages. Most progenitors were not labeled with any of the various combinations of antibodies used. Progenitors did not express markers characteristic for mature astroglia (GFAP), oligodendroglia (CNPase), or neurons (MAP2). Thus, although these progenitors are morphologically indistinguishable, a minority expresses markers of early neuronal or glial lineages, suggesting that they begin to differentiate during migration.
authors.affiliation: Department of Pathology, Division of Neuropathology, The Center for Neurobiology and Behavior, Columbia University College of Physicians and Surgeons, 630 W. 168th Street, New York, NY 10032, USA. am829@columbia.edu
authors.forename: Ana
authors.initials: A
authors.lastname: Milosevic
authors.forename: James E
authors.initials: JE
authors.lastname: Goldman
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000943:Antigens, Differentiation / Q000096:biosynthesis / Q000276:immunology*; D001253:Astrocytes / Q000166:cytology / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology*; D002465:Cell Movement; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D009474:Neurons / Q000166:cytology / Q000276:immunology; D009836:Oligodendroglia / Q000166:cytology / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000276:immunology
chemical_list: D000943:Antigens, Differentiation