Gene expression profiling using laser capture microdissection.
Human disease is governed by a complex array of cellular populations and sub-population. Gene expression profiling is proving an important means of understanding and classifying pathophysiologic processes by identifying genes, gene pathways and pathway networks not previously known to be associated with particular diseases. However, disease-associated gene expression can be obscured by surrounding 'normal' tissue. Laser capture microdissection allows gene expression analysis of pooled single cells, cell subpopulations and cell populations. Analysis of laser capture microdissection-procured cells will allow a better understanding of the cellular components of disease.
Todd
Randy
R
Department of Oral & Maxillofacial Surgery, Harvard School of Dental Medicine/Massachusetts General Hospital, 188 Longwood Avenue, Boston, MA, USA. Randy_todd@hms.harvard.edu
Lingen
Mark W
MW
Kuo
Winston P
WP
D018076:DNA, Complementary / Q000378:metabolism; D004210:Dissection; D015870:Gene Expression; D015723:Gene Library; D020287:In Situ Nick-End Labeling; D007834:Lasers; D008957:Models, Genetic; D025202:Molecular Diagnostic Techniques; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism
D018076:DNA, Complementary; D012313:RNA