title: The role of the Ets domain transcription factor Erm in modulating differentiation of neural crest stem cells.
abstract: The transcription factor Erm is a member of the Pea3 subfamily of Ets domain proteins that is expressed in multipotent neural crest cells, peripheral neurons, and satellite glia. A specific role of Erm during development has not yet been established. We addressed the function of Erm in neural crest development by forced expression of a dominant-negative form of Erm. Functional inhibition of Erm in neural crest cells interfered with neuronal fate decision, while progenitor survival and proliferation were not affected. In contrast, blocking Erm function in neural crest stem cells did not influence their ability to adopt a glial fate, independent of the glia-inducing signal. Furthermore, glial survival and differentiation were normal. However, the proliferation rate was drastically diminished in glial cells, suggesting a glia-specific role of Erm in controlling cell cycle progression. Thus, in contrast to other members of the Pea3 subfamily that are involved in late steps of neurogenesis, Erm appears to be required in early neural crest development. Moreover, our data point to multiple, lineage-specific roles of Erm in neural crest stem cells and their derivatives, suggesting that Erm function is dependent on the cell intrinsic and extrinsic context.
authors.affiliation: Institute of Cell Biology, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland.
authors.forename: Christian
authors.initials: C
authors.lastname: Paratore
authors.forename: Guya
authors.initials: G
authors.lastname: Brugnoli
authors.forename: Hye-Youn
authors.initials: HY
authors.lastname: Lee
authors.forename: Ueli
authors.initials: U
authors.lastname: Suter
authors.forename: Lukas
authors.initials: L
authors.lastname: Sommer
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D019070:Cell Lineage; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D016296:Mutagenesis; D009432:Neural Crest / Q000166:cytology*; D009457:Neuroglia / Q000166:cytology; D017434:Protein Structure, Tertiary; D051381:Rats; D013234:Stem Cells / Q000166:cytology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; C086680:ETV5 protein, human; C513243:Etv5 protein, rat; D014157:Transcription Factors