title: cDNA cloning and expression analysis of a novel human F-box only protein.
abstract: F-box proteins are an expanding family of eukaryotic proteins that are characterized by an approximately 40 amino acid motif. Some F-box proteins are critical for the controlled degradation of cellular regulatory proteins. During a large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a cDNA clone that encodes a novel F-box protein. It showed a 90.0% identity with the previously isolated mouse F-box protein16 at the amino acid level. Northern blot analysis showed no detectable expression, while reverse transcription-polymerase chain reaction analysis indicated that FBXO16 was expressed in the heart, spleen, and colon. By mapping, we localized the FBXO16 gene to the human chromosome 8p12. The FBXO16 gene consisted of 9 exons that spanned 67,816 bp of human genomic DNA.
authors.affiliation: State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Cheng
authors.forename: Yushu
authors.initials: Y
authors.lastname: Ma
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Ni
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Lingchen
authors.initials: L
authors.lastname: Guo
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Weiwen
authors.initials: W
authors.lastname: Xu
authors.forename: Gentao
authors.initials: G
authors.lastname: Cao
authors.forename: Chaoneng
authors.initials: C
authors.lastname: Ji
authors.forename: Kang
authors.initials: K
authors.lastname: Yin
authors.forename: Shaohua
authors.initials: S
authors.lastname: Gu
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Ma
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D018076:DNA, Complementary; D044783:F-Box Proteins; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D044783:F-Box Proteins; C473095:FBXO16 protein, human; D011506:Proteins