Inhibition of human hemoglobin autoxidation by sodium n-dodecyl sulphate.
The effect of sodium n-dodecyl sulphate (SDS) on hemoglobin autoxidation was studied in the presence of a 100 mM phosphate buffer (pH 7.0) by different methods. These included spectrophotometry, fluorescence technique, cyclic voltametry, differential scanning calorimetry, and densitometry. Spectroscopic studies showed that SDS concentrations up to 1 mM increased deoxy-, decreases oxy-, and had no significant effect on the met- conformation of hemoglobin. Therefore, a SDS concentration up to 1 mM increased the deoxy form of hemoglobin as the folded, compact state and decreases the oxy conformation. The turbidity measurements and differential scanning calorimetry techniques indicated a more stable conformation for hemoglobin in the presence of SDS up to 1 mM. Electrochemical studies also confirmed a more difficult oxidation under these conditions. The induction of the deoxy form in the presence of SDS was confirmed by densitometry techniques. The compact structure of deoxyhemoglobin blocks the formation of met-conformation in low SDS concentrations.
Reza
Dayer Mohammad
DM
Institute of Biochemistry and Biophysics, University of Tehran, Iran. moosavi@ibb.ut.ac.ir
Akbar
Moosavi-Movahedi Ali
MM
Parviz
Norouzi
N
Ghourchian
Hedayat-Olah
Shahrokh
Safarian
S
D003902:Detergents / Q000494:pharmacology; D004563:Electrochemistry; D006441:Hemoglobin A / Q000737:chemistry* / Q000187:drug effects*; D006454:Hemoglobins / Q000737:chemistry / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008706:Methemoglobin / Q000737:chemistry / Q000187:drug effects; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000737:chemistry / Q000187:drug effects; D011193:Potassium Iodide / Q000494:pharmacology; D011489:Protein Denaturation / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D003902:Detergents; D006454:Hemoglobins; D010108:Oxyhemoglobins; D012996:Solutions; D011193:Potassium Iodide; D012967:Sodium Dodecyl Sulfate; C100003:deoxyhemoglobin; D008706:Methemoglobin; D006441:Hemoglobin A