title: Macrophages, but not T and B lymphocytes, are critical for subepidermal blister formation in experimental bullous pemphigoid: macrophage-mediated neutrophil infiltration depends on mast cell activation.
abstract: Bullous pemphigoid (BP) is a subepidermal blistering disease associated with autoantibodies against two hemidesmosomal proteins, BP180 and BP230. Numerous inflammatory cells infiltrate the upper dermis in BP. We have previously shown by passive transfer studies that Abs to the ectodomain of murine BP180 are capable of triggering blisters in mice that closely mimic human BP. Experimental BP depends on complement activation and neutrophil infiltration. In the present study, we investigated the relative contribution of neutrophils, mast cells (MCs), macrophages (Mphi), and lymphocytes and their functional relationship in the immunopathogenesis of this disease model by using mice deficient in these cells. Wild-type, T cell-deficient, and T and B cell-deficient mice injected intradermally with pathogenic anti-murine BP180 IgG exhibited extensive subepidermal blisters. In contrast, mice deficient in neutrophils, MCs, and Mphi were resistant to experimental BP. MCs play a major role in neutrophil recruitment into the dermis. Furthermore, Mphi-mediated neutrophil infiltration depends on MC activation/degranulation.
authors.affiliation: Department of Dermatology, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Ruoyan
authors.initials: R
authors.lastname: Chen
authors.forename: Janet A
authors.initials: JA
authors.lastname: Fairley
authors.forename: Ming-Lang
authors.initials: ML
authors.lastname: Zhao
authors.forename: George J
authors.initials: GJ
authors.lastname: Giudice
authors.forename: Detlef
authors.initials: D
authors.lastname: Zillikens
authors.forename: Luis A
authors.initials: LA
authors.lastname: Diaz
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001768:Blister / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D004817:Epidermis / Q000276:immunology* / Q000473:pathology; D007074:Immunoglobulin G / Q000008:administration & dosage; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000473:pathology; D007271:Injections, Intradermal; D008264:Macrophages / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008819:Mice, Nude; D020556:Neutrophil Infiltration / Q000235:genetics / Q000276:immunology*; D024041:Non-Fibrillar Collagens; D010391:Pemphigoid, Bullous / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology*; D000097805:Collagen Type XVII
chemical_list: D001324:Autoantigens; D007074:Immunoglobulin G; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII