Arrhythmogenic peroxynitrite-induced alterations in mammalian heart contractility and its prevention with quercetin-filled liposomes.
The aim of the present study was to evaluate the effects of quercetin-filled phosphatidylcholine liposomes (PCLs) on peroxynitrite (ONOO-)-induced cardiac arrhythmias. Experiments were done using different experimental models, including isolated rat papillary muscle, Langendorff perfused rat hearts, and anesthetized animals. Being exogenously applied in a concentration greater than 50 microM, ONOO- caused inhibition of isometric twitch amplitude in isolated papillary muscles and led to an appearance of arrhythmias. Decomposed ONOO- had no similar effects and reversibly increased twitch amplitude. Authentic nitric oxide (NO, 100 microM) did not produce arrhythmias and had no significant effect on twitch amplitude. Verapamil and ruthenium red were with-out effect on ONOO- -induced arrhythmias, whereas tetrodotoxin and nicorandil effectively prevented arrhythmias development. Ouabain increased the arrhythmogenic effect of ONOO-. ONOO- significantly decreased coronary perfusion pressure (CPP) and mean left-ventricular pressure (MLVP) in the Langendorff perfused rat heart and produced severe arrhythmias. Authentic nitric oxide (NO) decreased CPP and MLVP insignificantly and resulted in a low incidence of arrhythmias. The NO donor SIN-1 in doses greater than 50 microM led to the appearance of low-incidence arrhythmias in anesthetized rats. Intraventricular injection of ONOO- promotes the appearance of a high incidence of arrhythmias in anesthetized rats and decreased MLVP. PCLs filled with the antioxidant quercetin restored normal cardiac contractility in both isolated tissues and anesthetizes animals. In conclusion, we hypothesized that ONOO-, but not its decomposed products, can initiate membrane lipid peroxidation and damage the phospholipid environment of ionic channels in myocardial cell plasma membranes inducing abnormal cardiac action potentials, arrhythmogenesis, and contractile dysfunction. Quercetin-filled PCL provide reliable protection against peroxynitrite-induced myocardial injury in isolated cardiac tissues and anesthetized animals primarily as a result of the decomposition of endogenously formed ONOO-.
Soloviev
A
A
Institute of Pharmacology and Toxicology, Academy of Medical Sciences, 03057 Kiev, Ukraine. s.a.pharm@naverex.kiev.ua
Stefanov
A
A
Parshikov
A
A
Khromov
A
A
Moibenko
A
A
Kvotchina
L
L
Balavoine
G
G
Geletii
Yu
Y
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000139:chemically induced* / Q000503:physiopathology* / Q000517:prevention & control; D001794:Blood Pressure / Q000187:drug effects; D003331:Coronary Vessels / Q000503:physiopathology; D004195:Disease Models, Animal; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004341:Drug Evaluation; D006352:Heart Ventricles / Q000503:physiopathology; D015994:Incidence; D008081:Liposomes; D008955:Models, Cardiovascular; D009200:Myocardial Contraction / Q000187:drug effects*; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control; D015428:Myocardial Reperfusion Injury / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control; D010210:Papillary Muscles / Q000187:drug effects / Q000503:physiopathology; D030421:Peroxynitrous Acid / Q000009:adverse effects*; D011794:Quercetin / Q000008:administration & dosage*; D051381:Rats; D011921:Rats, Inbred WKY; D012720:Severity of Illness Index; D017725:Ventricular Pressure / Q000187:drug effects
D008081:Liposomes; D030421:Peroxynitrous Acid; D011794:Quercetin