Nitrite accumulation and nitric oxide emission in relation to cellular signaling in nitrite reductase antisense tobacco.
An antisense nitrite reductase (NiR, EC 1.7.7.1) tobacco ( Nicotiana tabacum L.) transformant (clone 271) was used to gain insight into a possible correlation between nitrate reductase (NR, EC 1.6.6.1)-dependent nitrite accumulation and nitric oxide (NO(.)) production, and to assess the regulation of signal transduction in response to stress conditions. Nitrite concentrations of clone 271 leaves were 10-fold, and NO(.) emission rates were 100-fold higher than in wild type leaves. Increased protein tyrosine nitration in clone 271 suggests that high NO(.) production resulted in increased peroxynitrite (ONOO(-)) formation. Tyrosine nitration was also observed in vitro by adding peroxynitrite to leaf extracts. As in mammalian cells, NO(.) and derivatives also increased synthesis of proteins like 14-3-3 and cyclophilins, which are both involved in regulation of activity and stability of enzymes.
Morot-Gaudry-Talarmain
Y
Y
Laboratoire de Neurobiologie Cellulaire et Moléculaire, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette-Cedex, France.
Rockel
P
P
Moureaux
T
T
Quilleré
I
I
Leydecker
M T
MT
Kaiser
W M
WM
Morot-Gaudry
J F
JF
D048948:14-3-3 Proteins; D016375:Antisense Elements (Genetics) / Q000235:genetics; D002245:Carbon Dioxide / Q000378:metabolism; D021983:Cyclophilins / Q000096:biosynthesis; D050900:Ferredoxin-Nitrite Reductase; D008027:Light; D050895:Nitrate Reductase (NADH); D009565:Nitrate Reductases / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D009572:Nitrite Reductases / Q000235:genetics / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D030421:Peroxynitrous Acid / Q000378:metabolism / Q000494:pharmacology; D030821:Plants, Genetically Modified; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D014443:Tyrosine / Q000187:drug effects / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis
D048948:14-3-3 Proteins; D016375:Antisense Elements (Genetics); D009573:Nitrites; D002245:Carbon Dioxide; D030421:Peroxynitrous Acid; D009569:Nitric Oxide; D014443:Tyrosine; D014446:Tyrosine 3-Monooxygenase; D009565:Nitrate Reductases; D009572:Nitrite Reductases; D050895:Nitrate Reductase (NADH); D050900:Ferredoxin-Nitrite Reductase; D021983:Cyclophilins