Cyclosporin A therapy differently affects immunological-relevant gene expression following immunization.
We have studied the effect of cyclosporin A (CSA) on the expression of genes involved in the immune response in mice bearing a transgenic T cell receptor specific for the peptide 88-104 of the pigeon cytochrome c. Immunization of mice treated with CSA resulted in the blockade of the IL2, IFN-gamma, CXCR-5, CCR-5 and CD25 gene transcription. CSA decreased the density of CD69 on T-cells but did not interfere with the induction of the chemokine receptors CCR-1, CCR-4, CXCR2 and CXCR-4. Finally, CSA accelerated the kinetics of CD44 and CD62L expression or re-expression and increased the density of both markers on T-cell membrane. The present data show that priming in presence of CSA resulted in the acquisition of a particular phenotype by the activated T-cells.
Mascarell
Laurent
L
Unité Biologie des Populations Lymphocytaires, Department of Immunology, CNRS URA 1961, Institut Pasteur, F-75724 Paris Cedex 15, France.
Truffa-Bachi
Paolo
P
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000187:drug effects; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D003574:Cytochrome c Group / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D016207:Cytokines / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects*; D007114:Immunization; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology; D019707:Receptors, Chemokine / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D014158:Transcription, Genetic / Q000187:drug effects*
D015703:Antigens, CD; D003574:Cytochrome c Group; D016207:Cytokines; D007166:Immunosuppressive Agents; D011948:Receptors, Antigen, T-Cell; D019707:Receptors, Chemokine; D016572:Cyclosporine