A comparative study of an indirect fluorescent antibody test and an immunoperoxidase monolayer assay for the diagnosis of porcine proliferative enteropathy.
The currently used indirect fluorescent antibody test (IFAT) for the detection of antibodies against porcine proliferative enteropathy (PPE) was compared to an immunoperoxidase monolayer assay (IPMA). Serum samples used in this comparison were collected from 5-week-old pigs on day 0 (pre-experimental challenge) and on days 7, 14, 21, and 28 after oral inoculation with intestinal homogenate from pigs affected by PPE (28 challenged pigs) and sucrose phosphate glutamate solution (2 control pigs). All animals were euthanized 4 weeks after inoculation. Immunohistochemistry staining was applied to formalin-fixed, paraffin-embedded sections of ileum for the detection of Lawsonia intracellularis antigen. The serology results with each method agreed in all samples, except on days 0 and 7 in 1 control animal, which was positive by IPMA, but negative by IFAT. The percentage of agreement between IFAT and IPMA was 98.6%.
Guedes
Roberto M C
RM
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, Saint Paul 55108, USA.
Gebhart
Connie J
CJ
Winkelman
Nathan L
NL
Mackie-Nuss
Rebecca A
RA
D000818:Animals; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000379:methods*; D007410:Intestinal Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D020639:Lawsonia Bacteria / Q000276:immunology / Q000302:isolation & purification; D008297:Male; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology