title: Spectral imaging fluorescence microscopy.
abstract: The spectral resolution of fluorescence microscope images in living cells is achieved by using a confocal laser scanning microscope equipped with grating optics. This capability of temporal and spectral resolution is especially useful for detecting spectral changes of a fluorescent dye; for example, those associated with fluorescence resonance energy transfer (FRET). Using the spectral imaging fluorescence microscope system, it is also possible to resolve emitted signals from fluorescent dyes that have spectra largely overlapping with each other, such as fluorescein isothiocyanate (FITC) and green fluorescent protein (GFP).
authors.affiliation: CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, Japan.
authors.forename: Tokuko
authors.initials: T
authors.lastname: Haraguchi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimi
authors.forename: Takako
authors.initials: T
authors.lastname: Koujin
authors.forename: Noriyo
authors.initials: N
authors.lastname: Hashiguchi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hiraoka
mesh_terms: D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D016650:Fluorescein-5-isothiocyanate