title: Gene delivery to the rat liver using cationic lipid emulsion/DNA complex: comparison between intra-arterial, intraportal and intravenous administration.
abstract: OBJECTIVE
  To compare the efficiency of intra-arterial, intraportal, and intravenous administration of cationic lipid emulsion/DNA complex, as used for gene transfer to rat liver.
  
  
  METHODS
  DNA-carrier complex for the in-vivo experiment was prepared by mixing DNA and a cationic lipid emulsion. According to the administration route used (intra-arterial, intraportal, or intravenous), the animals were assigned to one of three groups. The heart, lung, liver, spleen and kidneys were removed and assayed for total protein and luciferase concentration.
  
  
  RESULTS
  The cationic lipid emulsion/DNA complex used successfully transfected the various organs via the different administration routes employed. Luciferase activity in each organ of untreated animals was negligible. Liver luciferase values were significantly higher in the groups in which intra-arterial or intraportal administration was used.
  
  
  CONCLUSIONS
  The intra-arterial or intraportal administration of cationic lipid emulsion/DNA complex is superior to intravenous administration and allows selective gene transfer to the liver.
authors.affiliation: Department of Radiology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Bo Yoon
authors.initials: BY
authors.lastname: Choi
authors.forename: Jin Wook
authors.initials: JW
authors.lastname: Chung
authors.forename: Jae Hyung
authors.initials: JH
authors.lastname: Park
authors.forename: Keon Ha
authors.initials: KH
authors.lastname: Kim
authors.forename: Young Il
authors.initials: YI
authors.lastname: Kim
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Koh
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Kwon
authors.forename: Kyoung Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Hyuk Jae
authors.initials: HJ
authors.lastname: Choi
authors.forename: Tae Woo
authors.initials: TW
authors.lastname: Kim
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Hesson
authors.initials: H
authors.lastname: Chung
authors.forename: Ik Chan
authors.initials: IC
authors.lastname: Kwon
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Jeong
mesh_terms: D000818:Animals; D002404:Catheterization; D002412:Cations / Q000008:administration & dosage*; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004655:Emulsions / Q000008:administration & dosage; D018014:Gene Transfer Techniques; D007269:Injections, Intra-Arterial; D007275:Injections, Intravenous; D008055:Lipids / Q000008:administration & dosage* / Q000235:genetics; D008099:Liver / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002412:Cations; D004655:Emulsions; D008055:Lipids; D004247:DNA