title: Antigenic cross-reactivity among avian pneumoviruses of subgroups A, B, and C at the matrix but not nucleocapsid proteins.
abstract: Earlier findings from our laboratory based on analysis of nucleotide and predicted amino acid sequence identities of 15 avian pneumoviruses (APVs) isolated from the United States (subgroup C) demonstrated that the viruses were phylogenetically separated from the European subgroup A and subgroup B viruses. Here, we investigated whether viruses from the three subgroups were cross-reactive by testing field sera positive for each of the APV subgroups in an enzyme-linked immunosorbent assay (ELISA) test with recombinant matrix (M) and nucleoprotein (N) proteins generated from a Minnesota APV isolate (APV/MN2A). Sera from turkeys infected with APV subgroup A, B, or C reacted with recombinant M protein derived from APV/MN2A. In contrast, recombinant N protein from APV/MN2A virus was reactive with sera from subtypes A and C viruses but not from subtype B virus. The results illustrate that viruses from the three APV subtypes share antigenic homology, and the M protein-based ELISA is adequate for monitoring APV outbreaks but not for distinguishing between different subtypes.
authors.affiliation: Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
authors.forename: Humphrey C M
authors.initials: HC
authors.lastname: Lwamba
authors.forename: David A
authors.initials: DA
authors.lastname: Halvorson
authors.forename: Kakambi V
authors.initials: KV
authors.lastname: Nagaraja
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Turpin
authors.forename: David
authors.initials: D
authors.lastname: Swayne
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Seal
authors.forename: M Kariuki
authors.initials: MK
authors.lastname: Njenga
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D015373:Bacterial Typing Techniques; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D019251:Nucleocapsid / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D018111:Pneumovirus / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000276:immunology; D014422:Turkeys; D014763:Viral Matrix Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins