Libraries of composite polyfluors built from fluorescent deoxyribosides.
We report on a new class of water-soluble fluorescent molecules (polyfluors) that are composed of multiple individual fluorophores assembled on a DNA-like backbone. Four fluorophore deoxyribosides were synthesized, and these individual molecules were assembled into oligofluor strings on a DNA synthesizer. A library of 256 tetrafluors was generated by split and pool methods on polystyrene beads. Images of the library under a fluorescence microscope revealed at least 40-50 different hues and intensities. Selected tetrafluors were resynthesized in pure form in solution and displayed properties, such as large Stokes shifts, that individual fluorophores do not have.
Gao
Jianmin
J
Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA.
Strässler
Christoph
C
Tahmassebi
Deborah
D
Kool
Eric T
ET
D004277:DNA, Single-Stranded / Q000737:chemistry; D003853:Deoxyribonucleosides / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D004277:DNA, Single-Stranded; D003853:Deoxyribonucleosides; D005456:Fluorescent Dyes; D011721:Pyrenes; C030984:pyrene