[Spin-spin interaction upon introduction of a spin label into immunoglobulins M and G at the carbohydrate moiety].
By spin labeling the monoclonal IgM and normal IgG at the carbohydrate moiety with 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl, preparations were obtained whose ESR spectra indicate rapid exchange spin-spin interactions between two spin labels. It was shown that, in the case of spin-labeled IgM, this spectrum is determined by a glycopeptide noncovalently bound to IgM, which incorporates two spin labels.
Timofeev
V P
VP
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 117984 Russia.
Nikol'skiĭ
D O
DO
Lapuk
V A
VA
Aleshkin
V A
VA
D002850:Chromatography, Gel; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D007074:Immunoglobulin G / Q000737:chemistry*; D007075:Immunoglobulin M / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009844:Oligosaccharides; D013113:Spin Labels; C015527:4-amino-TEMPO