title: Activation of K+ Channels in the Plasma Membrane of Arabidopsis by ATP Produced Photosynthetically.
abstract: Light activates a K+ channel and transiently depolarizes the plasma membrane of Arabidopsis mesophyll cells. Genetically or chemically impairing photosynthesis abolished this electrical response to light. These results indicate that illuminated chloroplasts produce a factor that activated K+ channels in the plasma membrane. By patch clamping at the single-channel level, we have obtained evidence that ATP is one such factor. Application of 0.2 to 2 mM ATP to the cytoplasmic side of excised patches of membrane reversibly activated the type of channel that was activated by light in cell-attached patches. In addition, an outward-rectifying K+ channel and an outward-rectifying nonselective cation channel were similarly activated by ATP, whereas a nonselective stretch-activated channel was unaffected by this treatment. This novel mechanism for controlling the permeability of the plasma membrane to K+ may be important to photosynthetic metabolism.
authors.affiliation: Department of Biology, Yale University, P.O. Box 6666, New Haven, Connecticut 06511.
authors.forename: E. P.
authors.initials: EP
authors.lastname: Spalding
authors.forename: MHM.
authors.initials: M
authors.lastname: Goldsmith