Structural biology of viruses by the combination of electron cryomicroscopy and X-ray crystallography.
Recent developments in electron cryomicroscopy and image analysis have made it a powerful tool to investigate the structure, assembly, and dynamics of biological supramolecular assemblies. The subjects of study now include a variety of biological samples that may be homogeneous or heterogeneous, symmetric or nonsymmetric. The combination of this technique with X-ray crystallography plays an increasingly important role in structural biology and provides unique structural information for understanding large, complex biological systems. Here we provide an overview of the technologies and specific applications to virus structure and function.
Tang
Liang
L
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Johnson
John E
JE
D020285:Cryoelectron Microscopy / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods*; D012270:Ribosomes / Q000737:chemistry / Q000648:ultrastructure / Q000821:virology; D013329:Structure-Activity Relationship; D014764:Viral Proteins / Q000737:chemistry / Q000648:ultrastructure; D014780:Viruses / Q000737:chemistry* / Q000648:ultrastructure*
D014764:Viral Proteins