title: Secretion of alpha-amylase from Pseudoalteromonas haloplanktis TAB23: two different pathways in different hosts.
abstract: Secretion of cold-adapted alpha-amylase from Pseudoalteromonas haloplanktis TAB23 was studied in three Antarctic bacteria. We demonstrated that the enzyme is specifically secreted in the psychrophilic hosts even in the absence of a protein domain that has been previously reported to be necessary for alpha-amylase secretion in Escherichia coli. The occurrence of two different secretion pathways in different hosts is proposed.
authors.affiliation: Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II--Complesso Universitario Monte Sant'Angelo, Naples, Italy. tutino@unina.it
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Tutino
authors.forename: Ermenegilda
authors.initials: E
authors.lastname: Parrilli
authors.forename: Laura
authors.initials: L
authors.lastname: Giaquinto
authors.forename: Angela
authors.initials: A
authors.lastname: Duilio
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sannia
authors.forename: Georges
authors.initials: G
authors.lastname: Feller
authors.forename: Gennaro
authors.initials: G
authors.lastname: Marino
mesh_terms: D000864:Antarctic Regions; D003080:Cold Temperature; D020563:Gammaproteobacteria / Q000201:enzymology* / Q000235:genetics; D005822:Genetic Vectors; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D000516:alpha-Amylases