title: Libraries of composite polyfluors built from fluorescent deoxyribosides.
abstract: We report on a new class of water-soluble fluorescent molecules (polyfluors) that are composed of multiple individual fluorophores assembled on a DNA-like backbone. Four fluorophore deoxyribosides were synthesized, and these individual molecules were assembled into oligofluor strings on a DNA synthesizer. A library of 256 tetrafluors was generated by split and pool methods on polystyrene beads. Images of the library under a fluorescence microscope revealed at least 40-50 different hues and intensities. Selected tetrafluors were resynthesized in pure form in solution and displayed properties, such as large Stokes shifts, that individual fluorophores do not have.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Gao
authors.forename: Christoph
authors.initials: C
authors.lastname: Strässler
authors.forename: Deborah
authors.initials: D
authors.lastname: Tahmassebi
authors.forename: Eric T
authors.initials: ET
authors.lastname: Kool
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry; D003853:Deoxyribonucleosides / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D004277:DNA, Single-Stranded; D003853:Deoxyribonucleosides; D005456:Fluorescent Dyes; D011721:Pyrenes; C030984:pyrene