title: [Determination of lignan glycosides in the roots of Gaultheria yunnanensis Rehd. by HPLC].
abstract: OBJECTIVE
  To determine the contents of four lignan glycosides in the root of Gaultheria yunnanensis, namely, D1: gaultheroside A, D2:(-)-8'-methoxyisolariciresinol-2a-O-beta-D-xylopyranoside, D3:(-)-isolariciresinol-2a-O-beta-D-xylopyranside, D4:(+)-lyoniresinol-2a-O-beta-D-glucoxylopyranoside.
  
  
  METHODS
  Rp-HPLC was used to separate and analyze the lignan glycosides in the root of the title plant.
  
  
  RESULTS
  The method for sample preparation was set up; the gradient program for the mobile phase was established; and the retention times of four lignan glycosides were determined.
  
  
  CONCLUSIONS
  The method has been proved to be accurate, reproducible and of higher recovery rate.
authors.affiliation: School of Pharmaceutical Sciences, Beijing Medical University, Beijing 100083.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: D
authors.initials: D
authors.lastname: Guo
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Zheng
authors.forename: K
authors.initials: K
authors.lastname: Koike
authors.forename: Z
authors.initials: Z
authors.lastname: Jia
authors.forename: T
authors.initials: T
authors.lastname: Nikaido
authors.forename: G
authors.initials: G
authors.lastname: Fan
authors.forename: Y
authors.initials: Y
authors.lastname: Duan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D031282:Gaultheria / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis*; D017705:Lignans / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; D017705:Lignans; C471257:gaultheroside A