title: Configurational entropy and mechanical properties of cross-linked polymer chains: implications for protein and RNA folding.
abstract: We discuss the statistical mechanical properties of a single polymer chain that forms cross links among its monomers. Models of this type have served as prototypes in theories of RNA and protein folding. The chain is allowed to form pseudoknots and its monomers can each participate in multiple cross links. We demonstrate that the conformational free energy of such a chain can be estimated by using an algorithm that scales as a power of the number of cross links N(N1-N3, depending on the problem). Straightforward exact evaluation of the chain partition function via multidimensional integration scales exponentially with N and often is computationally prohibitive. Our approach can also be used to compute the "entropic force" generated by a cross-linked chain when it is stretched at its ends. Such forces can be directly measured by atomic force microscopy or by laser optical trap experiments performed on single RNA, DNA, and protein molecules.
authors.affiliation: Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA. marakov@mail.cm.utexas.edu
authors.forename: Dmitrii E
authors.initials: DE
authors.lastname: Makarov
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Rodin
mesh_terms: D001696:Biomechanical Phenomena; D001704:Biopolymers / Q000737:chemistry*; D003432:Cross-Linking Reagents; D019277:Entropy; D008958:Models, Molecular; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D017510:Protein Folding; D012313:RNA / Q000737:chemistry
chemical_list: D001704:Biopolymers; D003432:Cross-Linking Reagents; D012313:RNA