title: Rapid isolation of cancer cells from tumor tissue by micromanuiplator and extraction of tiny amount of RNA.
abstract: OBJECTIVE
  To establish a rapid method for isolating and purifying cancer cells from tumor tissue and for RNA extraction from tiny amount of the cells thus obtained.
  
  
  METHODS
  Frozen sections of the tumor tissues were prepared followed by rapid staining. Clusters of the cancer cells were isolated from the sections by micromanipulation technique and purified for extracting intact RNA that was subsequently assayed.
  
  
  RESULTS
  Clear vision was achieved by the staining of the sections. The cancer cell clusters were precisely isolated from which high-quality intact RNA was obtained as demonstrated by reverse transcriptase-PCR.
  
  
  CONCLUSIONS
  Micromanuiplation can be effectively used in stead of laser capture microdissection to isolate and purify targeted cells from tiny amount of tissue samples, therefore making RNA extraction possible in this context.
authors.affiliation: Department of Pathology, First Military Medical University, Guangzhou 510515, China.
authors.forename: Pei-Zhou
authors.initials: PZ
authors.lastname: Jiang
authors.forename: Xin-Ming
authors.initials: XM
authors.lastname: Shen
authors.forename: Hua
authors.initials: H
authors.lastname: Huang
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Shi
authors.forename: Kai-Tai
authors.initials: KT
authors.lastname: Yao
mesh_terms: D004587:Electrophoresis, Agar Gel / Q000379:methods; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology; D004947:Esophagus / Q000473:pathology; D006801:Humans; D008846:Micromanipulation / Q000379:methods; D012334:RNA, Neoplasm / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D012334:RNA, Neoplasm