[The formation of the influenza virus aggregates, enriched with hemagglutinin].
The formation of electrostatic aggregates was studied by analysis of two types of virus-containing liquids: initial warm liquid collected at temperature 37 degrees and the same liquid stored over the night at temperature 4 degrees C. The formation of virus aggregates was revealed at 4 degrees C. The aggregates formed at temperature 4 degrees C had a relatively high HA/NP ratio in comparison with unassociated virus analyzed at 37 degrees. HA-enriched aggregates were found in the precipitate formed under short-term high-speed centrifugation as well as in "heavy arm" of the virus profile in the saccharose gradient. Aggregates formed at 4 degrees C dissociated at 37 degrees. The ability to form aggregates is reversible and correlates with the virus concentration. It is shown also that virus containing liquid contains heterogenic structures with molecular weight under 2000 kD having HA involved in the forming aggregates enriching HA. Possible nature of low-molecular HA-containing structures involved in the aggregates and nature of relations stabilizing aggregates are discussed.
Prokudina
E N
EN
Semenova
N P
NP
Chumakov
V M
VM
Rudneva
I A
IA
Iamnikova
S S
SS
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D004285:Dogs; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000032:analysis*; D029521:Alphainfluenzavirus / Q000276:immunology*; D011864:Radioimmunosorbent Test; D013395:Sucrose; D013696:Temperature
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D013395:Sucrose