title: Comparison of four reverse transcription-polymerase chain reaction procedures for the detection of dengue virus in clinical specimens.
abstract: The sensitivity of dengue virus identification by mosquito inoculation and four reverse transcription-polymerase chain reaction (RT-PCR) procedures (Am. J. Trop. Med. Hyg. 45 (1991) 418 (H); J. Clin. Microbiol. 29 (1991) 2107 (M); J. Clin. Microbiol. 30 (1992) 545 (L); and Southeast Asian J. Trop. Med. Public Health 27 (1996) 228 (Y)) were compared using coded clinical specimens derived from areas in Thailand where all four dengue serotypes circulate. The sensitivity of virus detection in serologically confirmed dengue cases was 54, 52, 60, 79, and 80% for mosquito inoculation, procedures H, M, L and Y, respectively. In comparison to clinical specimens which yielded virus isolates by mosquito inoculation, there was relatively low sensitivity in detecting each of the four dengue serotypes by PCR: procedure H-dengue 4 (25%), procedure M-dengue 3 (73%), procedure L-dengue 1 (70%), and procedure Y-dengue 1 (79%). Dengue virus was detectable by RT-PCR for more days of illness and in the presence of dengue-specific antibody when compared to virus isolated in mosquitoes. Procedures L and Y were more sensitive than mosquito inoculation or procedures H and M in detecting all four dengue serotypes in clinical specimens and may be the RT-PCR methods of choice for virus surveillance or research use.
authors.affiliation: Department of Virology, US Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. fnbrs@diamond.mahidol.ac.th
authors.forename: Boonyos
authors.initials: B
authors.lastname: Raengsakulrach
authors.forename: Ananda
authors.initials: A
authors.lastname: Nisalak
authors.forename: Niwat
authors.initials: N
authors.lastname: Maneekarn
authors.forename: Pa-Thai
authors.initials: PT
authors.lastname: Yenchitsomanus
authors.forename: Chandhana
authors.initials: C
authors.lastname: Limsomwong
authors.forename: Aroonroong
authors.initials: A
authors.lastname: Jairungsri
authors.forename: Vipa
authors.initials: V
authors.lastname: Thirawuth
authors.forename: Sharone
authors.initials: S
authors.lastname: Green
authors.forename: Siripen
authors.initials: S
authors.lastname: Kalayanarooj
authors.forename: Saroj
authors.initials: S
authors.lastname: Suntayakorn
authors.forename: Nopporn
authors.initials: N
authors.lastname: Sittisombut
authors.forename: Prida
authors.initials: P
authors.lastname: Malasit
authors.forename: David
authors.initials: D
authors.lastname: Vaughn
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology / Q000635:transmission; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D016133:Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013785:Thailand
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M