Photosensitizing effect of protoporphyrin IX in pigmented melanoma of mice.
No fluorescence of protoporphyrin IX (PpIX) was measured using a fiber optic probe in pigmented B16F10 melanoma in mice after topical application of 5-aminolevulinic acid methylester (ALA-Me). However, chemical extraction of tissues excised from mice after intratumoral administration of ALA-Me or its parent compound ALA revealed that this tumor had the capability to produce PpIX. Small amounts of endogenous porphyrins, mainly PpIX, were found in the melanoma not treated with these drugs. Topical application of ALA-Me followed by exposure with laser light (633nm) delayed the growth of the tumors slightly. Light alone also had a significant effect on the tumor growth.
Juzenas
Petras
P
Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, NO-0310 Montebello, Oslo, Norway. petras.juzenas@labmed.uio.no
Juzeniene
Asta
A
Stakland
Silje
S
Iani
Vladimir
V
Moan
Johan
J
D000287:Administration, Topical; D000408:Alamethicin / Q000627:therapeutic use; D000622:Aminolevulinic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D018327:Hutchinson's Melanotic Freckle / Q000188:drug therapy / Q000473:pathology; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D011524:Protoporphyrins / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D012878:Skin Neoplasms / Q000188:drug therapy* / Q000473:pathology; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured
D017319:Photosensitizing Agents; D011524:Protoporphyrins; C114767:delta-aminolevulinic acid methyl ester; D000408:Alamethicin; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX