title: Identification of 1088 new transposon insertions of Caenorhabditis elegans: a pilot study toward large-scale screens.
abstract: We explored the feasibility of a strategy based on transposons to generate identified mutants of most Caenorhabditis elegans genes. A total of 1088 random new insertions of C. elegans transposons Tc1, Tc3, and Tc5 were identified by anchored PCR, some of which result in a mutant phenotype.
authors.affiliation: CGMC, CNRS-UMR 5534, Université Lyon1, 69100 Villeurbanne, France.
authors.forename: Edwige
authors.initials: E
authors.lastname: Martin
authors.forename: Hélène
authors.initials: H
authors.lastname: Laloux
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Couette
authors.forename: Thierry
authors.initials: T
authors.lastname: Alvarez
authors.forename: Catherine
authors.initials: C
authors.lastname: Bessou
authors.forename: Oliver
authors.initials: O
authors.lastname: Hauser
authors.forename: Satis
authors.initials: S
authors.lastname: Sookhareea
authors.forename: Michel
authors.initials: M
authors.lastname: Labouesse
authors.forename: Laurent
authors.initials: L
authors.lastname: Ségalat
mesh_terms: D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D017931:DNA Primers; D004251:DNA Transposable Elements; D005091:Exons; D009153:Mutagens; D010865:Pilot Projects
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D009153:Mutagens