title: Serine repeat antigen (SERA5) is predominantly expressed among the SERA multigene family of Plasmodium falciparum, and the acquired antibody titers correlate with serum inhibition of the parasite growth.
abstract: The Plasmodium falciparum serine repeat antigen (SERA) is one of the blood stage malaria vaccine candidates. The malaria genome project has revealed that SERA is a member of the SERA multigene family consisting of eight SERA homologues clustered on chromosome 2 and one SERA homologue on chromosome 9. Northern blotting and real time quantitative reverse transcription-PCR with five independent parasite strains, including three allelic representative forms of the SERA gene, have shown that all of the SERA homologues are transcribed most actively at trophozoite and schizont stages and that SERA5 (SERA/SERP) is transcribed predominantly among the family. Polyclonal antibodies were raised against recombinant proteins representing the N-terminal portions of four significantly transcribed SERA homologues (SERA3 to -6) in the center of the cluster on chromosome 2. Using these antibodies, indirect immunofluorescence microscopy detected the expression of SERA3 to -6, with similar localization, in all trophozoite- and schizont-infected erythrocytes. We have examined 40 sera from Ugandan adults for their antibody reactivity and found that enzyme-linked immunosorbent assay titer against SERA5 N-terminal domain, but not against other SERA proteins, is positively correlated with the inhibition of in vitro parasite growth by individual sera. Our data confirm the usefulness of the N-terminal domain of SERA5 as a promising malaria candidate vaccine.
authors.affiliation: Department of Molecular Protozoology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Aoki
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Sawako
authors.initials: S
authors.lastname: Itagaki
authors.forename: Brenda A
authors.initials: BA
authors.lastname: Okech
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Egwang
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Matsuoka
authors.forename: Nirianne Marie Q
authors.initials: NM
authors.lastname: Palacpac
authors.forename: Toshihide
authors.initials: T
authors.lastname: Mitamura
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Horii
mesh_terms: D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis* / Q000235:genetics*; D015152:Blotting, Northern; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D017780:Malaria Vaccines / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D005810:Multigene Family; D010957:Plasmids / Q000378:metabolism; D010963:Plasmodium falciparum / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D000953:Antigens, Protozoan; D017780:Malaria Vaccines; D012333:RNA, Messenger; D011994:Recombinant Proteins