Differential display analysis of gene expression in the hippocampus, marginal division and other related brain areas: a preliminary study.
OBJECTIVE
To investigate the difference in the expression profiles in the hippocampus, marginal division and other related brain areas for identification of genes specific to the hippocampus and marginal division.


METHODS
Fetal hippocampus, marginal division, caudate putamen and amygdala were obtained and mRNA differential display technique was employed to study the gene expression in the 4 brain areas.


RESULTS
Two differentially expressed fragments were isolated from each of the hippocampus and marginal division, and cloning and sequence analysis of one of the fragment 277 bp in length from the hippocampus showed total homology with the sequence of human clone AC0049.


CONCLUSIONS
The 4 brain areas have different gene expression profiles.
Ning
Qun
Q
Institute of Neuroscience, First Military Medical University, Guangzhou 510282, China.
Shu
Si-Yun
SY
Bao
Xin-Min
XM
D001921:Brain / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D015870:Gene Expression; D020869:Gene Expression Profiling; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger