title: Nitric oxide and tumor necrosis factor-alpha production by Ixeris dentata in mouse peritoneal macrophages.
abstract: Using mouse peritoneal macrophages, we have examined the mechanism by which Ixeris dentata (IXD) regulates nitric oxide (NO) production. When IXD was used in combination with recombinant interferon-gamma (rIFN-gamma), there was a marked cooperative induction of NO production. However, IXD had no effect on NO production by itself. The increased production of NO from rIFN-gamma plus IXD-stimulated cells was almost completely inhibited by pre-treatment with pyrrolidine dithiocarbamate (PDTC), an inhibitor of nuclear factor kappa B (NF-kappaB). Furthermore, treatment with IXD alone or rIFN-gamma plus IXD in peritoneal macrophages caused a significant increase in tumor necrosis factor-alpha (TNF-alpha) production. PDTC decreased TNF-alpha production induced by IXD significantly. These findings demonstrate that IXD increases the production of NO and TNF-alpha by rIFN-gamma-primed macrophages and suggest that NF-kappaB plays a critical role in mediating these effects of IXD.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Korea Institute of Oriental Pharmacy, Wonkwang University, Iksan, Chonbuk, South Korea.
authors.forename: Hwan-Suck
authors.initials: HS
authors.lastname: Chung
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Mi-Jung
authors.initials: MJ
authors.lastname: Han
authors.forename: Seung-Taeck
authors.initials: ST
authors.lastname: Park
authors.forename: Kang-Kyung
authors.initials: KK
authors.lastname: Seong
authors.forename: Seung-Hwa
authors.initials: SH
authors.lastname: Baek
authors.forename: Dong-Myong
authors.initials: DM
authors.lastname: Jeong
authors.forename: Myong Jo
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000818:Animals; D019659:Asteraceae; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide