Dimethylhydrazine: a reliable positive control for the short sampling time in the UDS assay in vivo.
In the first international guideline addressing the unscheduled DNA synthesis (UDS) assay in vivo (OECD guideline no. 486, adopted July 1997) only the genotoxic liver carcinogen N-nitrosodimethylamine (NDMA) is proposed as positive control for the short sampling time. Since NDMA is extremely volatile, alternative positive controls should be identified to facilitate handling and reduce exposure risk during routine testing. At Bayer AG and at RCC-CCR GmbH, the genotoxic but non-volatile dimethylhydrazine (DMH; as dihydrochloride) was used instead as positive control in livers of Wistar rats and to a limited extent of NRMI mice after 2-4h exposure. As shown by the data presented in this paper DMH induced a positive result in a total of 21 UDS in vivo studies over a period of 7 years. A negative result was never seen for DMH. Due to these results DMH was proven to be a suitable and reliable positive control in the UDS assay in vivo. Consequently, DMH should be considered as positive control for the short sampling time in the next issue of OECD guideline no. 486.
Brendler-Schwaab
Susanne Y
SY
Toxicology, Bayer AG, D-42096, Wuppertal, Germany. susanne.brendler-schwaab.sb@bayer-ag.de
Völkner
Wolfgang
W
Fautz
Rolf
R
Herbold
Bernd A
BA
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002478:Cells, Cultured / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D004261:DNA Replication; D004127:Dimethylhydrazines / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009152:Mutagenicity Tests; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012588:Scintillation Counting
D002273:Carcinogens; D004127:Dimethylhydrazines; D004247:DNA