title: Influence of amino acid side chain packing on Fe-methionine coordination in thermostable cytochrome C.
abstract: Paramagnetic NMR and optical studies of the oxidized forms of mesophile Pseudomonas aeruginosa cytochrome c(551) and its quintuple mutant (F7A/V13M/F34Y/E43Y/V78I), and thermophile Hydrogenobacter thermophilus cytochrome c(552) demonstrated that the amino acid side chain packings in the protein interior influence the coordination bond between the heme iron and the axial methionine in the proteins. The strength of heme axial coordinations was found to correlate with the overall protein thermostability.
authors.affiliation: Department of Chemistry, University of Tsukuba, Tsukuba 305-8571, Japan. yamamoto@staff.chem.tsukuba.ac.jp
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Norifumi
authors.initials: N
authors.lastname: Terui
authors.forename: Naoki
authors.initials: N
authors.lastname: Tachiiri
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Minakawa
authors.forename: Hitomi
authors.initials: H
authors.lastname: Matsuo
authors.forename: Tsunenori
authors.initials: T
authors.lastname: Kameda
authors.forename: Jun
authors.initials: J
authors.lastname: Hasegawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sambongi
authors.forename: Susumu
authors.initials: S
authors.lastname: Uchiyama
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Igarashi
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D003574:Cytochrome c Group / Q000737:chemistry*; D006091:Gram-Negative Chemolithotrophic Bacteria / Q000737:chemistry; D006418:Heme / Q000737:chemistry; D006358:Hot Temperature; D007501:Iron / Q000737:chemistry*; D008715:Methionine / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003574:Cytochrome c Group; D006418:Heme; D008715:Methionine; D007501:Iron