Cloning and chromosomal localization of MYO15A to chromosome 5 of the dog (Canis familiaris).
Mutations in the myosin XVA gene (MYO15A) cause congenital non-syndromic deafness in humans and mice. Therefore, the M YO15A gene represents a candidate gene for hereditary hearing loss in dogs. Using a human cDNA to screen a dog BAC library, we isolated a canine BAC clone. Sequencing of the BAC ends confirmed homology to the human gene. To facilitate future linkage studies, we report the physical mapping of the canine MYO15A gene to CFA5q23-q24 by FISH and RH mapping.
Rak
Simone G
SG
Institute of Animal Breeding and Genetics, School of Veterinary Medicine Hannover, Germany.
Drögemüller
Cord
C
Kuiper
Heidi
H
Leeb
Tosso
T
Quignon
Pascale
P
André
Catherine
C
Distl
Ottmar
O
D000818:Animals; D002871:Chromosome Banding; D003001:Cloning, Molecular; D004285:Dogs; D016678:Genome; D006319:Hearing Loss, Sensorineural / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009154:Mutation; D009218:Myosins / Q000235:genetics*; D020161:Physical Chromosome Mapping; D021101:Radiation Hybrid Mapping
C485245:MYO15A protein, human; D009218:Myosins