title: Detection and characterization of pediatric serum antibody to the OMP P5-homologous adhesin of nontypeable Haemophilus influenzae during acute otitis media.
abstract: We reported earlier that antibody in sera collected from seven children with acute otitis media (AOM) due to nontypeable Haemophilus influenzae (NTHI) recognized immunodominant regions of P5-fimbrin just as we had observed in a chinchilla model of experimental NTHI-induced AOM. To expand upon those preliminary findings, we further characterized pediatric serum antibodies directed against this adhesin during AOM. Collectively, the data show that children respond immunologically to P5-fimbrin and they do so in a manner that allows for the distinction of sequence diversity within short linear peptides representing a focused region of this surface-exposed protein. The immune recognition we observed encourages us to further develop a P5-fimbrin based vaccine component.
authors.affiliation: Department of Pediatrics, College of Medicine & Public Health, Children's Research Institute, The Ohio State University, Rm W302, 700 Children's Drive, Columbus, OH 43205-2696, USA.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Novotny
authors.forename: Michael E
authors.initials: ME
authors.lastname: Pichichero
authors.forename: Philippe A
authors.initials: PA
authors.lastname: Denoël
authors.forename: Cecil
authors.initials: C
authors.lastname: Neyt
authors.forename: Sylvie
authors.initials: S
authors.lastname: Vanderschrick
authors.forename: Guy
authors.initials: G
authors.lastname: Dequesne
authors.forename: Lauren O
authors.initials: LO
authors.lastname: Bakaletz
mesh_terms: D000208:Acute Disease; D018829:Adhesins, Bacterial / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D010861:Fimbriae, Bacterial; D006193:Haemophilus influenzae / Q000276:immunology*; D006801:Humans; D007223:Infant; D010033:Otitis Media / Q000276:immunology* / Q000382:microbiology
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C082912:outer membrane protein P5, Haemophilus