Mitochondrial DNA restriction map for the Caribbean fruit fly, Anastrepha suspensa, and occurrence of mitochondrial DNA diversity within highly inbred colonies.
A restriction map has been constructed for Anastrepha suspensa mitochondrial DNA. One HaeIII site was found to be polymorphic among individuals in highly inbred colonies and a feral population. Based on mapping information, the polymorphic site was determined to be in the ATPase 6 gene. Primers TK-J-3804 and C3-N-5460 amplified this region. The amplicon was cut by HaeIII in flies of one haplotype and not cut in flies of the other haplotype. From 30 to 43% of the individual flies studied had this additional HaeIlI site. After cloning of the approximately 5200 bp XbaI fragment, the two mitotypes were identified. A 988 base fragment, coding for the entire tRNA-Lys(AAG), tRNA-Asp(GAC), and ATPase 8genes, and a partial ATPase 6gene was sequenced Four silent mutations, including the one at the informative site were located. The HaeIII polymorphism and other sequence differences may prove useful as a diagnostic for identification of the origin of introduced fruit flies.
Heath
M
M
USDA-ARS, National Germplasm Repository, Subtropical Horticulture Research Station, Miami, Florida 33158, USA. mheath@saa.ars.usda.gov
Kuhn
D
D
Schnell
R
R
Olano
C
C
D000818:Animals; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D007178:Inbreeding; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D033621:Tephritidae / Q000235:genetics*
D004272:DNA, Mitochondrial