[Changes in serum concentration of podophyllotoxin after its topical application with liposome on rat skin].
OBJECTIVE
To investigate the changes of serum concentration of podophyllotoxin after topical application of liposome podophyllotoxin suspension on rat skin.


METHODS
SD rats were used in this study, which were divided into test group (n=48) to receive application of liposome podophyllotoxin (0.5 % ) suspension and control group (n=48) treated with 0.5 % podophyllotoxin alcohol solution. Blood samples were obtained from the heart at 1, 2, 4, 6, 8, 10, 12 and 24 h respectively after drug application, and the serum concentration of podophyllotoxin was determined by spectrofluorometry.


RESULTS
The area under the curve of plasma drug concentration of the control group was 2.3-fold greater than that of the test group. Eight hours after drug application, the serum concentration of podophyllotoxin reached the peak in the test group, while in the control group, only two hours was needed to reach the peak. The peak serum level of podophyllotoxin in the test group were significantly lower than that of the control group (166.395 +/- 14.634 ng/ml vs 378.603 +/- 26.105 ng/ml, P<0.001).


CONCLUSIONS
The systemic absorption of podophyllotoxin in rats after its topical application in liposome suspension is significantly lower than that after application of 0.5 % podophyllotoxin alcohol solution, therefore the systemic toxicity may be reduced.
Zhang
San-quan
SQ
Department of Dermatology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Zeng
Kang
K
Jiang
Bin-bin
BB
Li
Jian-hua
JH
Xie
Yu-sheng
YS
Zhu
Xiao-liang
XL
Li
Guo-feng
GF
D000279:Administration, Cutaneous; D000465:Algorithms; D000818:Animals; D019540:Area Under Curve; D005260:Female; D008081:Liposomes; D008297:Male; D011034:Podophyllotoxin / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D013050:Spectrometry, Fluorescence
D008081:Liposomes; D011034:Podophyllotoxin