title: Glucocorticoid inhibits expression of V-1, a catecholamine biosynthesis regulatory protein, in cultured adrenal medullary cells.
abstract: V-1 acts as a positive and coordinate regulator of gene expression of catecholamine biosynthetic enzymes in PC12D cells. The present study was conducted to investigate the mechanism controlling expression of V-1 in the adrenal gland. Immunohistochemical analysis demonstrated that noradrenergic chromaffin cells more highly expressed V-1 than adrenergic chromaffin cells preferentially expressing the glucocorticoid receptor in rat adrenal glands. Western blotting showed that in cultured bovine adrenal medullary cells, dexamethasone, a synthetic glucocorticoid, inhibited expression of V-1, and that this inhibition was prevented by RU-486, a glucocorticoid receptor antagonist. These results suggest that adrenal expression of V-1 is differentially controlled by glucocorticoids through the specific receptor, and that thereby V-1 regulates catecholamine biosynthesis in a catecholaminergic phenotype-dependent manner.
authors.affiliation: Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Hiwatashi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kurahashi
authors.forename: Risa
authors.initials: R
authors.lastname: Hatada
authors.forename: Susumu
authors.initials: S
authors.lastname: Ueno
authors.forename: Takao
authors.initials: T
authors.lastname: Honma
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yanagihara
authors.forename: Haruko
authors.initials: H
authors.lastname: Yanase
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Iwanaga
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ohizumi
authors.forename: Tohru
authors.initials: T
authors.lastname: Yamakuni
mesh_terms: D000313:Adrenal Medulla / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000187:drug effects / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D005938:Glucocorticoids / Q000494:pharmacology*; D006727:Hormone Antagonists / Q000494:pharmacology; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D015735:Mifepristone / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D005938:Glucocorticoids; D006727:Hormone Antagonists; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D011965:Receptors, Glucocorticoid; C065700:myotrophin; D015735:Mifepristone; D003907:Dexamethasone; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D009638:Norepinephrine; D004837:Epinephrine