Effect of hemodialysis on the plasma level of type IV collagenases and their inhibitors.
OBJECTIVE
Increased cell expression of matrix metalloproteinase-9 (MMP-9) was associated with the development of atherosclerosis and osseoarticular tissue destruction in hemodialysis patients. In this study, the pre- and post-HD plasma concentrations of type IV collagenases and their inhibitors in HD patients were examined.


METHODS
Commercial ELISA kits and Zymography techniques were used to assay the parameters in 40 patients pre- and post-HD session.


RESULTS
After the hemodialysis process, MMP-9, MMP-2 and TIMP-2 levels were 124 +/- 72, 706 +/- 242 and 248 +/- 90 ng/mL, significantly different from the pre-HD values (187 +/- 148, 759 +/- 304 and 43 +/- 14 ng/mL). TIMP-1 were not affected by HD. Female subjects and patients with chronic glomerulonephritis had higher TIMP-2 than their counterparts (p < 0.05). The effect of gender on MMP-2 levels was interacted with that of membrane types (p < 0.01).


CONCLUSIONS
These results indicated that the hemodialysis process tends to decrease the overall activity of the peripheral plasma MMP system in HD patients.
Chou
Fen-Pi
FP
Institute of Biochemistry, Chung Shan Medical University, Taichung 402, Taiwan.
Chu
Shu-Chen
SC
Cheng
Ming-Chun
MC
Yang
Shun-Fa
SF
Cheung
Wing-Nam
WN
Chiou
Hui-Ling
HL
Hsieh
Yih-Shou
YS
D000328:Adult; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood*; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D061965:Matrix Metalloproteinase Inhibitors; D008875:Middle Aged; D006435:Renal Dialysis; D013997:Time Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000097:blood*
D061965:Matrix Metalloproteinase Inhibitors; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9