title: Regulation of the Osmotin Gene Promoter.
abstract: By introducing a chimeric gene fusion of the osmotin promoter and [beta]-glucuronidase into tobacco by Agrobacterium-mediated transformation, we have demonstrated a very specific pattern of temporal and spatial regulation of the osmotin promoter during normal plant development and after adaptation to NaCl. We have found that the osmotin promoter has a very high natural level of activity in mature pollen grains during anther dehiscence and in pericarp tissue at the final, desiccating stages of fruit development. GUS activity was rapidly lost after pollen germination. The osmotin promoter thus appears to be unique among active pollen promoters described to date in that it is active only in dehydrated pollen. The osmotin promoter was also active in corolla tissue at the onset of senescence. Adaptation of plants to NaCl highly stimulated osmotin promoter activity in epidermal and cortex parenchyma cells in the root elongation zone; in epidermis and xylem parenchyma cells in stem internodes; and in epidermis, mesophyll, and xylem parenchyma cells in developed leaves. The spatial and temporal expression pattern of the osmotin gene appears consistent with both osmotic and pathogen defense functions of the gene.
authors.affiliation: Center for Plant Environmental Stress Physiology, 1165 Department of Horticulture, Purdue University, West Lafayette, Indiana 47907-1165.
authors.forename: A. K.
authors.initials: AK
authors.lastname: Kononowicz
authors.forename: D. E.
authors.initials: DE
authors.lastname: Nelson
authors.forename: N. K.
authors.initials: NK
authors.lastname: Singh
authors.forename: P. M.
authors.initials: PM
authors.lastname: Hasegawa
authors.forename: R. A.
authors.initials: RA
authors.lastname: Bressan