title: Increased stability of human growth hormone with reduced lactogenic potency.
abstract: Human growth hormone (hGH), whose main function is the somatic growth stimulation, induces diverse effects including lactation. We examined the possibility of hGH stabilization by elimination of its lactogenic activity. Chimeric GHs were constructed by replacement of different segments of hGH with sequences derived from non-lactogenic porcine GH. As was observed in the rat Nb2-11C lymphoma cell test, lactogenic activity of some chimeric hormones was seriously destroyed. This kind of hormones displayed the substantial increase in thermal and guanidine hydrochloride stability. The more stable hGH variants were found to be more soluble in Escherichia coli cells.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 17/10, Moscow 117997, Russia.
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Schulga
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Makarov
authors.forename: Iliya V
authors.initials: IV
authors.lastname: Levichkin
authors.forename: Yuliya V
authors.initials: YV
authors.lastname: Belousova
authors.forename: Vladimir M
authors.initials: VM
authors.lastname: Lobachov
authors.forename: Irina I
authors.initials: II
authors.lastname: Protasevich
authors.forename: C Nick
authors.initials: CN
authors.lastname: Pace
authors.forename: Mikhail P
authors.initials: MP
authors.lastname: Kirpichnikov
mesh_terms: D000818:Animals; D002460:Cell Line; D004355:Drug Stability; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D013006:Growth Hormone / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D019791:Guanidine; D006358:Hot Temperature; D019382:Human Growth Hormone / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D002479:Inclusion Bodies / Q000378:metabolism; D007774:Lactation / Q000187:drug effects; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D012995:Solubility; D013552:Swine
chemical_list: D011993:Recombinant Fusion Proteins; D019382:Human Growth Hormone; D013006:Growth Hormone; D019791:Guanidine