Effect of topical iganidipine on experimental elevation of aqueous flare induced by prostaglandin E(2) and EP agonists in pigmented rabbits.
We evaluated the role of topical iganidipine on experimental aqueous flare elevation in rabbits. Transcorneal diffusion of prostaglandin E(2) (PGE(2)), 25 microg/ml or 7.09 x 10(-2) mmol/l, or highly selective agonists for prostaglandin E(2) receptor subtypes (EP), 25 microg/ml, was achieved with the use of a glass cylinder to produce aqueous flare elevation in pigmented rabbits. Iganidipine was topically administered before application of PGE(2) or EP agonists. Aqueous flare was measured with a laser flare cell meter. Topical instillation of 0.1% iganidipine once or twice inhibited 64 +/- 8% (p < 0.01) and 84 +/- 9% (p < 0.01) of PGE(2)-induced aqueous flare elevation, respectively. Two instillations of 0.1% iganidipine inhibited 95 +/- 5% (p < 0.01) of EP2-agonist(ONO-AE1-259-01)-induced flare elevation and 98 +/- 3% (p < 0.01) of EP4-agonist(ONO-AE1-392)-induced flare rise. Topical iganidipine may have anti-inflammatory activity in the eye.
Yanagisawa
Shuichiro
S
Department of Ophthalmology, Toyama Medical and Pharmaceutical University, Toyama, Japan. ophthal@ms.toyama-mpu.ac.jp
Hayasaka
Seiji
S
Zhang
Xue-Yun
XY
Hayasaka
Yoriko
Y
Nagaki
Yasunori
Y
D000287:Administration, Topical; D000818:Animals; D001082:Aqueous Humor / Q000187:drug effects*; D002121:Calcium Channel Blockers / Q000008:administration & dosage* / Q000494:pharmacology; D015232:Dinoprostone / Q000819:agonists*; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology; D010879:Piperazines / Q000008:administration & dosage* / Q000494:pharmacology; D020033:Protein Isoforms / Q000819:agonists; D011725:Pyridines / Q000008:administration & dosage* / Q000494:pharmacology; D011817:Rabbits; D018078:Receptors, Prostaglandin E / Q000819:agonists*
D002121:Calcium Channel Blockers; D008738:Methyl Ethers; C461399:ONO-AE1-329; D010879:Piperazines; D020033:Protein Isoforms; D011725:Pyridines; D018078:Receptors, Prostaglandin E; C108540:iganidipine; D015232:Dinoprostone