Angiopoietin 1 promotes tumor angiogenesis and tumor vessel plasticity of human cervical cancer in mice.
Angiopoietins have been increasingly implicated to play important roles in blood vessel formation, remodeling, maturation, and maintenance. However, their roles in tumor angiogenesis and hence tumor growth and metastasis still remain uncertain. In this work, angiopoietin 1 expression was amplified in human cervical cancer HeLa cells by stable transfection or recombinant human adenovirus-mediated gene transfer. We show that increased angiopoietin 1 expression promoted in vivo growth of human cervical cancers in mice by promoting tumor angiogenesis and inhibiting tumor cell apoptosis. Furthermore, we also show for the first time that overexpression of angiopoietin 1 also leads to increased tumor vessel plasticity with a large number of vessels lacking periendothelial supporting cells. These results indicate that angiopoietin 1 promotes tumor angiogenesis and tumor vessel plasticity of human cervical cancer in mice.
Shim
Winston S N
WS
Molecular Angiogenesis Laboratory, Department of Biological Sciences, National University of Singapore, 119260, Singapore.
Teh
Ming
M
Bapna
Akanksha
A
Kim
Injune
I
Koh
Gou-Young
GY
Mack
Peter O P
PO
Ge
Ruowen
R
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D042683:Angiopoietin-1; D000818:Animals; D001808:Blood Vessels / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000378:metabolism; D005260:Female; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D008222:Lymphokines / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009389:Neovascularization, Pathologic / Q000503:physiopathology*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
C467630:ANGPT1 protein, human; D042683:Angiopoietin-1; C467628:Angpt1 protein, mouse; D016228:Endothelial Growth Factors; D008222:Lymphokines; D008562:Membrane Glycoproteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors