Application of restriction display PCR in preparing the probes of the gene chip for investigating gene expression profile of K562 cells.
OBJECTIVE
To collect the gene fragments of the gene chips for investigating the expression profile of K562 cells.


METHODS
The mRNA was extracted from cultured K562 cells to obtain cDNA by reverse transcription-PCR. The gene fragment products of different groups were acquired and isolated by reverse display PCR (RD-PCR).


RESULTS
The probes for the gene chip were successfully prepared.


CONCLUSIONS
RD-PCR adapted in this study suits the needs for collecting the probes for the gene chips.
Mao
Xiang-Ming
XM
Key Laboratory of Biochips of PLA, Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
Ma
Wen-Li
WL
Jiang
Li
L
Zheng
Wen-Ling
WL
D015342:DNA Probes; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D020014:K562 Cells; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods*
D015342:DNA Probes