Comparison of melatonin versus vitamin C on oxidative stress and antioxidant enzyme activity in Alzheimer's disease induced by okadaic acid in neuroblastoma cells.
We demonstrated that exposure of cells to 50 nM okadaic acid for 2 h induced a reduction in cellular glutathione transferase, glutathione reductase and catalase activity. Likewise, this acid prompted an increase in lipid peroxidation. Treatment of cells with 10(-5) M melatonin or 0.5 microg/ml vitamin C prevented the effects of okadaic acid. These results indicate that okadaic acid induces an oxidative stress imbalance, while melatonin and vitamin C prevent the oxidative stress induced by okadaic acid. Likewise, these data indicate the great importance of oxidative stress in both this experimental model and in the development and course of neurodegenerative disease, especially Alzheimer's disease. They show that melatonin is much more efficient than vitamin C in reducing the extent of oxidative stress. This phenomenon was demonstrated by the smaller dose of melatonin needed to obtain effects similar to those obtained with vitamin C on lipid peroxidation and by the protective effect of melatonin on antioxidant enzyme activity.
Montilla-López
Pedro
P
Department of Biochemistry and Molecular Biology, School of Medicine, University of Córdoba, Avda Menéndez Pidal s/n, 14004 Córdoba, Spain. bb1molop@uco.es
Muñoz-Agueda
M Carmen
MC
Feijóo López
Montserrat
M
Muñoz-Castañeda
Juan R
JR
Bujalance-Arenas
Inmaculada
I
Túnez-Fiñana
Isaac
I
D000544:Alzheimer Disease / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D005982:Glutathione Transferase / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008550:Melatonin / Q000494:pharmacology*; D051379:Mice; D009447:Neuroblastoma / Q000188:drug therapy / Q000201:enzymology*; D019319:Okadaic Acid / Q000009:adverse effects / Q000037:antagonists & inhibitors*; D018384:Oxidative Stress / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D019319:Okadaic Acid; D002374:Catalase; D005982:Glutathione Transferase; D008550:Melatonin; D001205:Ascorbic Acid