Water structuring centers of mammalian cell surfaces.
Cultured mammalian cells appeared to express specific particles on their surface, which could be detected by their ability to nucleate ice crystals (I-centers) in a newly developed, two-dimensional crystallization assay. Their expression required approximately 24 h independent of cell density, and metabolic energy, and the number and distribution of the I-centers were cell-type specific. Their characteristic ability to nucleate ice crystals was highly sensitive to dehydration, to hyaluronidase and phospholipase C, but not to a number of proteases such as trypsin, chymotrypsin, collagenase, and pronase. However, these proteases, especially pronase, were able to detach the I-centers from the cell surface, without destroying their ability to nucleate ice crystals. I-centers were specific products of live cells, located in relatively small numbers at the cell surface organized in a detachable, sheet-like structure. We propose to consider the ice nucleating ability of I-centers as an expression of their ability to influence the water structure in the surface of cells. Even though their biological function is not known at this time, as water-structuring centers they appear remarkable enough to warrant our attention.
Albrecht-Buehler
Guenter
G
Department of Cell and Molecular Biology, Northwestern University Medical School, 303 E Chicago Avenue, Chicago, Illinois 60611, USA.
D016475:3T3 Cells; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D003198:Computer Simulation; D003460:Crystallization; D005612:Freeze Drying / Q000379:methods*; D006801:Humans; D007053:Ice; D051379:Mice; D011142:Polyvinyl Alcohol / Q000737:chemistry; D016707:Tissue Fixation / Q000379:methods; D014867:Water / Q000737:chemistry*
D007053:Ice; D014867:Water; D011142:Polyvinyl Alcohol