A preliminary study of chromium distribution in chromium-rich brewer's yeast cell by NAA.
The purpose of this study was to assess the chromium (Cr) distribution in chromium-rich brewer's yeast cell. The chromium concentrations in the cell wall and protoplast fractions of the chromium-rich yeast were determined by neutron activation analysis (NAA). Moreover, the combined state of chromium and amino acid content in the Cr-rich brewer's yeasts was analyzed and measured. The experimental results indicate that the introduction of water-soluble chromium(III) salt as a component of the culture medium for yeasts results in a substantial amount of chromium absorbed through the cell wall by the yeast, among which 80.9% are accumulated in the protoplast. It implies that, under optimal conditions, yeasts are capable of accumulating large amounts of chromium and incorporating chromium into organic compounds.
Ding
W J
WJ
Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, People's Republic of China.
Qian
Q F
QF
Hou
X L
XL
Feng
W Y
WY
Chen
C Y
CY
Chai
Z F
ZF
Zhang
B R
BR
Wang
K
K
D000596:Amino Acids / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D002857:Chromium / Q000032:analysis*; D009501:Neutron Activation Analysis / Q000379:methods*; D011523:Protoplasts / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000166:cytology*
D000596:Amino Acids; D002857:Chromium