Molecular cloning of DAX1 and SHP cDNAs and their expression patterns in the Nile tilapia, Oreochromis niloticus.
Piscine DAX1 and SHP cDNAs with an open reading frame encoding 296 and 258 amino acid residues, respectively, as well as SHP partial gene fragment, were cloned from Nile tilapia. Phylogenetic analyses of DAX1s, SHPs, and homologous EST fragments indicate that DAX1 and SHP are conserved in gene structure and are present throughout vertebrates. A single band of approximately 1.4kb for DAX1 and of approximately 1.2kb for SHP was detected in the Northern blot analysis. Tissue distribution analysis by RT-PCR showed that fish DAX1 and SHP mRNAs are widely expressed in adult tissues, with the most abundant expression in gonads and liver, respectively. DAX1 and SHP were also detected in gonads of both sexes at 5-90 days after hatching (dah). However, the expression of DAX1 is weak at 5 and 10dah and then significantly up-regulated between 10 and 15dah, whereas the expression of SHP is moderate and consistent during the ontogeny.
Wang
De-Shou
DS
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan.
Kobayashi
Tohru
T
Senthilkumaran
Balasubramanian
B
Sakai
Fumie
F
Sudhakumari
Cheni Chery
CC
Suzuki
Taiga
T
Yoshikuni
Michiyasu
M
Matsuda
Masaru
M
Morohashi
Ken-ichirou
K
Nagahama
Yoshitaka
Y
D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D057137:DAX-1 Orphan Nuclear Receptor; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns / Q000235:genetics; D008322:Mammals; D008969:Molecular Sequence Data; D010802:Phylogeny; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000235:genetics*; D018168:Receptors, Retinoic Acid / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000737:chemistry; D012097:Repressor Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D017210:Tilapia / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
D000199:Actins; D057137:DAX-1 Orphan Nuclear Receptor; D017931:DNA Primers; D004268:DNA-Binding Proteins; C539965:NR0B1 protein, human; D018160:Receptors, Cytoplasmic and Nuclear; D018168:Receptors, Retinoic Acid; D011994:Recombinant Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C099713:nuclear receptor subfamily 0, group B, member 2