Differential processing of the Xenopus ATP(CTP):tRNA nucleotidyltransferase mRNA.
The ATP(CTP):tRNA nucleotidyltransferase (CCA-adding enzyme) adds CCA to the 3(') end of immature or damaged tRNAs. It is reported on here the cloning, expression analysis, and functional characterization of the Xenopus CCA-adding enzyme, XCCA (GenBank Accession #AF466151). It is demonstrated that XCCA adds cytosine and adenosine residues to the ends of prepared tRNA and is therefore a functional CCA-adding enzyme. XCCA is encoded by a rare mRNA present at less than 0.001% of the cellular mRNA in all adult tissues examined. The mRNA is expressed as two transcripts of 1.5 and 2.3kb, generated through differential utilization of two transcription start sites and two 3' cleavage and polyadenylation sites. Utilization of the most 5' transcription initiation site produces an mRNA encoding a putative mitochondrial import sequence. It is anticipated that the Xenopus oocyte will be an excellent system for analyzing the regulation of XCCA expression and the intracellular targeting of the XCCA enzyme.
Keady
Brian T
BT
Biology Department, Boston College, 428 Higgins Hall, 140 Commonwealth Avenue, 02467, Chestnut Hill, MA 02467, USA.
Attfield
Kathleen R
KR
Hake
Laura E
LE
D000241:Adenosine / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D003062:Codon / Q000235:genetics; D017124:Conserved Sequence; D003570:Cytidine Triphosphate / Q000378:metabolism*; D003596:Cytosine / Q000378:metabolism; D017931:DNA Primers; D018076:DNA, Complementary; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008322:Mammals; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D016133:Polymerase Chain Reaction; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014981:Xenopus
D003062:Codon; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D003570:Cytidine Triphosphate; D003596:Cytosine; D000255:Adenosine Triphosphate; D012316:RNA Nucleotidyltransferases; C023198:tRNA nucleotidyltransferase; D000241:Adenosine