Genetic variability in Hoplias malabaricus (Osteichthyes: Erythrinidae) in fluvial and lacustrine environments in the Upper Paraná river floodplain (Paraná State, Brazil).
Genetic variability in Hoplias malabaricus, from two localities in the upper Paraná Riverfloodplain, was investigated by starch and polyacrylamide gel electrophoresis. A total of 52 specimens were analyzed for 14 enzymatic systems. Twenty-three gene loci of 13 enzymatic systems (AAT, ACP, ADH, GDH, G6PDH, GPI, IDH, LDH, MDH, MEP, PGM, PER, and SOD) were analyzed by starch gel electrophoresis (Penetrose-30). The EST system was analyzed by polyacrylamide gel electrophoresis, and one polymorphic locus was found (EST-1). Twenty-four loci were detected. The proportion of polymorphic loci was 37.5% in the lagoon and 33.3% in the river. Significant differences in allele frequencies of five loci were found between specimens from the two environments. Expected mean heterozygosity (He = 0.14) is the same in the river and lagoon, however, Nei's genetic distance (D) between the population of the two locations was 0.049.
Peres
Maria Dolores
MD
Nupelia, Núcleo de Pesquisas em Limnologia, Ictiologia e Aqüicultura).
Renesto
Erasmo
E
Lapenta
Ana Sílvia
AS
Zawadzki
Cláudio Henrique
CH
D000818:Animals; D001938:Brazil; D017753:Ecosystem; D004592:Electrophoresis, Starch Gel; D004798:Enzymes / Q000235:genetics*; D005399:Fishes / Q000235:genetics* / Q000254:growth & development; D005618:Fresh Water; D014644:Genetic Variation; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism
D004798:Enzymes; D007527:Isoenzymes