Number of levels needed for diagnosis of cervical biopsies.
BACKGROUND
Three levels of histologic sections are routinely prepared for small biopsies in many surgical pathology laboratories. The first level is superficial and may not be representative of the entire biopsy, and may therefore represent wasted resources and time for technologists and pathologists alike.


OBJECTIVE
To determine if disposing of the first of 3 standard levels materially affects the diagnosis of cervical biopsies.


METHODS
We retrospectively reviewed levels 2 and 3 of 241 cervical biopsies and compared the review diagnosis with the original diagnosis, using 6 diagnostic categories: I, benign lesions; II, human papillomavirus-associated changes or low-grade dysplasia; III, high-grade dysplasia/carcinoma in situ; IV, invasive carcinoma; V, insufficient tissue for diagnosis; and VI, further workup needed. If there was a discrepancy between the original and review diagnostic categories, then we examined level 1 to determine if this would resolve the disagreement.


METHODS
The surgical pathology laboratory.


METHODS
Women undergoing cervical biopsy.


METHODS
None.


METHODS
The frequency with which level 1 information changed the diagnostic category determined with levels 2 and 3 only.


RESULTS
After review of levels 2 and 3, the diagnosis in 42 (17%) of 241 cases was in disagreement with the original diagnosis. Upon review of level 1, the review category was changed to that of the original diagnosis in only one case.


CONCLUSIONS
The first of 3 levels contributed little to reaching a diagnosis in these cervical biopsies. Control of interobserver variation would seem to be superior to preparation of additional levels as a strategy for reducing diagnostic error.
Luo
Yehung Vivian
YV
Department of Pathology, University of Texas Health Science Center at San Antonio, 78229, USA.
Prihoda
Thomas J
TJ
Sharkey
Francis E
FE
D001706:Biopsy; D003951:Diagnostic Errors / Q000517:prevention & control*; D005260:Female; D006801:Humans; D015588:Observer Variation; D010340:Pathology, Surgical / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results; D012189:Retrospective Studies; D013048:Specimen Handling / Q000379:methods*; D002577:Uterine Cervical Diseases / Q000473:pathology*