A novel structure-specific endonuclease activity associated with polypyrimidine-tract binding (PTB) related protein from rat testis.
Nucleases are involved in the processing of various intermediates generated during crucial DNA metabolic processes such as replication, repair, and recombination and also during maturation of RNA precursors. An endonuclease, degrading specifically single-stranded circular DNA, was identified earlier in rat testis nuclear extract while purifying a strand-transfer activity. We are now reporting the purification of this endonuclease, which is a monomeric 42 kDa protein, from rat testis to near-homogeneity. In addition to degrading single-stranded circular DNA, it nicks supercoiled plasmid DNA to generate relaxed DNA and does not act on linear single-stranded or double-stranded DNA. It also makes specific incisions at the single-strand/duplex junction of pseudo-Y, 3'- and 5'-overhangs and 3'- and 5'-flap structures. Other structures such as mismatch, insertion loop, and Holliday junction are not substrates for the testis endonuclease. In contrast to FEN1, the testis endonuclease makes asymmetric incisions on both strands of the branched structures, and free single-stranded ends are not necessary for the structure-specific incisions. Neither 5'-3' nor 3'-5' exonuclease activity is associated with the testis endonuclease. The amino acid sequences of tryptic peptides of the 42 kDa endonuclease show near-identity to polypyrimidine-tract binding protein (PTB) that is involved in the regulation of splicing of eukaryotic mRNA. The significance of the results on the association of structure-specific endonucleae activities with PTB-related protein is discussed.
Haldar
Devyani
D
Department of Biochemistry, Indian Institute of Science, Bangalore-560012, India.
Acharya
Samir
S
Rao
M R S
MR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004270:DNA, Circular / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D004720:Endonucleases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000737:chemistry; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000737:chemistry; D005092:Exonucleases / Q000737:chemistry; D006868:Hydrolysis; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D038941:Polypyrimidine Tract-Binding Protein / Q000037:antagonists & inhibitors / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013737:Testis / Q000201:enzymology*
D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004791:Enzyme Inhibitors; D038941:Polypyrimidine Tract-Binding Protein; D004720:Endonucleases; D005090:Exodeoxyribonucleases; D005092:Exonucleases; D043211:Exodeoxyribonuclease V; C120663:structure-specific endonuclease I