title: [Determination of gallic acid in garden burnet by HPLC].
abstract: OBJECTIVE
  To establish a HPLC method for the determination of gallic acid in garden burnet.
  
  
  METHODS
  The sample was extracted with methanol under ultrasonic management for 25 min. Chromatographic conditions: Shimadzu LC-6A with SPD-6AV detector at 270 nm and auto injector SIL-6A, methanol-0.025 mol/L phosphoric acid(15:85) as the mobile phase. Shin-pack CLC-ODS column(6.0 mm x 15 mm).
  
  
  RESULTS
  Gallic acid in garden burnet was baseline separated and determined. The average content was 0.22%, recovery rate 97.4% and relative standard deviation 1.3%.
  
  
  CONCLUSIONS
  Simple, rapid and accurate, the method provides new indexes for quality control of Garden Burnet.
authors.affiliation: Liaoning College of Traditional Chinese Medicine, Shenyang 110032.
authors.forename: M
authors.initials: M
authors.lastname: Sha
authors.forename: A
authors.initials: A
authors.lastname: Cao
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Yang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005707:Gallic Acid / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D019685:Rosales / Q000737:chemistry*
chemical_list: D005707:Gallic Acid