DCMU inhibits in vivo nitrate reduction in illuminated barley (C(3)) leaves but not in maize (C(4)): a new mechanism for the role of light?
The leaves of C(4) plants possess a superior metabolic efficiency not only in terms of photosynthetic carbon assimilation, but also in terms of inorganic nitrogen assimilation, when compared to C(3)plants. In vivo nitrate assimilation efficiency of leaves is dependent on light, but the obligatory presence of light has been debated and its role remains confounded. This problem has not been addressed from the standpoint of the C(3) vs. C(4) nature of the species investigated, which may actually hold the key to resolve the controversy. Here, we present the first report providing evidence for differential photo-regulation of leaf nitrate reduction in barley ( Hordeum vulgare L.) vs. maize ( Zea mays L.) plants, which may help explain the superior nitrogen-use efficiency (and hence superior productivity) of maize plants. The novel finding that carbohydrate-depleted maize leaves were able to reduce nitrate when photosynthesis was inhibited by 3-(3',4'-dichlorophenyl)-1,1'-dimethylurea (DCMU) in the presence of light, raises a very important question about the possibilities of a new photo-regulatory mechanism for supporting nitrate reduction in maize leaves operating independently of photosynthetic carbon dioxide fixation. On the other hand, leaves of barley could not carry out any in vivo nitrate assimilation, whatsoever, under these conditions. We find another fundamental difference between the two species in terms of differential regulation of nitrate reductase (NR; EC 1.6.6.1). In barley leaves, NR activity and activation state remained unaffected due to DCMU, but in sharp contrast, both were appreciably upregulated in maize. Collectively, the results indicate that enzyme capacity is not limiting for nitrate reduction in leaves, as the NR activity was higher in barley than in maize. The maize leaves may have had a selective advantage due to C(4) morphology/metabolism in terms of maintaining a better reductant/carbon skeleton supply for nitrate reduction.
Basra
Amarjit S
AS
Department of Agronomy and Range Science, University of California, Davis 95616, USA.
Dhawan
Ashok K
AK
Goyal
Sham S
SS
D002245:Carbon Dioxide / Q000378:metabolism; D004237:Diuron / Q000494:pharmacology*; D006540:Herbicides / Q000494:pharmacology; D001467:Hordeum / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008027:Light; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010788:Photosynthesis / Q000187:drug effects / Q000502:physiology; D045322:Photosynthetic Reaction Center Complex Proteins / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D010944:Plants / Q000145:classification / Q000378:metabolism; D003313:Zea mays / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects
D006540:Herbicides; D009566:Nitrates; D045322:Photosynthetic Reaction Center Complex Proteins; D002245:Carbon Dioxide; D004237:Diuron; D009565:Nitrate Reductases; D050901:Nitrate Reductase