A new spectrofluorometric method for the determination of ascorbic acid based on its activating effect on a hemoglobin-catalyzed reaction.
A spectrofluorometric method for the determination of ascorbic acid (AA) based on its activation on the hemoglobin-catalyzed reaction was proposed. The fluorescence intensity of the product was measured under the optimal experimental conditions, i.e. 4.0 x 10(-6) M H2O2, 6.0 x 10(-5) M p-cresol, 1.2 M NH3-NH4Cl (pH 10.4) and 2.0 x 10(-7) M hemoglobin. The order of additions of the reagents was also studied. The activation of AA was found to be associated with a high ammonia concentration. The linear range of the method was 9.0 x 10(-10)-3.6 x 10(-8) M of AA. The detection limit was calculated to be 3.0 x 10(-10) M. The relative standard deviation of this method is 1.6% at 7.0 x 10(-9) M for 11 determinations.
Wang
Ruiyong
R
Department of Chemistry, Wuhan University, PR China.
Liu
Zhihong
Z
Cai
Ruxiu
R
Li
Xiaoyan
X
D000641:Ammonia; D001205:Ascorbic Acid / Q000032:analysis* / Q000494:pharmacology*; D002021:Buffers; D002384:Catalysis / Q000187:drug effects; D005453:Fluorescence; D006454:Hemoglobins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013607:Tablets / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D002021:Buffers; D006454:Hemoglobins; D013607:Tablets; D000641:Ammonia; D001205:Ascorbic Acid