[Improved three-dimension reconstruction of serial frozen tissue sections of the temporal bone of guinea pigs].
OBJECTIVE
To improve the serial frozen tissue sections of the temporal bone of guinea pigs for better three-dimension reconstruction of the computer images.


METHODS
The temporal bone of the guinea pigs was first decalcified by formate before embedding with lines marking the position of the specimen in glutin at low temperature.


CONCLUSIONS
Thinner slices, shorter period and more accurate orientation were achieved to highlight the spatial features of the temporal bone, thus facilitating the anatomical study of the temporal bone.
Huang
Wei
W
Henan Occupational Training Medical College, Zhengzhou 450003, China.
Dong
Ming-min
MM
Liu
Hong-jian
HJ
D000818:Animals; D005629:Frozen Sections / Q000379:methods*; D006168:Guinea Pigs; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D013701:Temporal Bone / Q000033:anatomy & histology*