[Identification of differentially expressed genes in bone marrow CD34+ cells by suppression subtractive hybridization].
OBJECTIVE
To understand the differential gene expression profiles between bone marrow cells and mobilized peripheral blood CD34(+) cells.


METHODS
Suppression subtractive hybridization (SSH) was employed to identify the genes differentially expressed in bone marrow and mobilized peripheral blood CD34(+) cells obtained from a healthy donor.


RESULTS
Twenty-one differentially expressed genes were identified that could be categorized into S-phase- or G(2)-M cell cycle-related genes and CCAAT/enhancer binding protein (C/EBP) transcription factor family. The genes highly expressed in bone marrow CD34(+) cells indicated low expression of their counterparts as SSH demands strict paired comparison between the 2 types of CD34(+) cells of different origins.


CONCLUSIONS
CD34(+) cells derived from bone marrow are featured by more active proliferation than are those from mobilized peripheral blood.
Chen
Jie
J
Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. chinsyou@263.net
Zhou
Shu-yun
SY
Feng
Ru
R
D018952:Antigens, CD34 / Q000097:blood*; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*
D018952:Antigens, CD34