title: [Improved three-dimension reconstruction of serial frozen tissue sections of the temporal bone of guinea pigs].
abstract: OBJECTIVE
  To improve the serial frozen tissue sections of the temporal bone of guinea pigs for better three-dimension reconstruction of the computer images.
  
  
  METHODS
  The temporal bone of the guinea pigs was first decalcified by formate before embedding with lines marking the position of the specimen in glutin at low temperature.
  
  
  CONCLUSIONS
  Thinner slices, shorter period and more accurate orientation were achieved to highlight the spatial features of the temporal bone, thus facilitating the anatomical study of the temporal bone.
authors.affiliation: Henan Occupational Training Medical College, Zhengzhou 450003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Ming-min
authors.initials: MM
authors.lastname: Dong
authors.forename: Hong-jian
authors.initials: HJ
authors.lastname: Liu
mesh_terms: D000818:Animals; D005629:Frozen Sections / Q000379:methods*; D006168:Guinea Pigs; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D013701:Temporal Bone / Q000033:anatomy & histology*