title: A novel ubiquitin carboxyl terminal hydrolase is involved in toad oocyte maturation.
abstract: p28, a 28kD protein from toad (Bufo bufo gargarizans) oocytes, was identified by using p13(suc1)-agarose affinity chromatography. Sequence homology analysis of the full-length cDNA of p28 (Gene Bank accession number: AF 314091) indicated that it encodes a protein containing 224 amino-acids with about 55% identities and more than 70% positives to human, rat or mouse UCH-L1, and contains homological functional domains of UCH family. Anti-p28 monoclonal antibody, on injecting into the oocytes, could inhibit the progesterone-induced resumption of meiotic division in a dose-dependent manner. The recombinant protein p28 showed similar SDS/PAGE behaviors to the native one, and promoted ubiquitin ethyl ester hydrolysis, a classical catalytic reaction for ubiquitin carboxyl terminal hydrolases (UCHs). The results in this paper reveal that a novel protein, p28, exists in the toad oocytes, is a UCH L1 homolog, was engaged in the process of progesterone-induced oocyte maturation possibly through an involvement in protein turnover and degradation.
authors.affiliation: National Laboratory of Contraceptives and Devices Research, Shanghai Institute of Planned Parenthood Research, China
authors.forename: Zhao Gui
authors.initials: ZG
authors.lastname: Sun
authors.forename: Wei Hua
authors.initials: WH
authors.lastname: Kong
authors.forename: Yan Jun
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Shan
authors.initials: S
authors.lastname: Yan
authors.forename: Ji Ning
authors.initials: JN
authors.lastname: Lu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Gu
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.forename: Jia Ke
authors.initials: JK
authors.lastname: Tso
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002023:Bufo bufo / Q000196:embryology*; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D005260:Female; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009865:Oocytes / Q000737:chemistry / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D014452:Ubiquitins / Q000737:chemistry*
chemical_list: D014452:Ubiquitins; D002265:Carboxylic Ester Hydrolases