[Changes of neuropeptide Y mRNA expression in the cerebral tissues of rat models of subarachnoid hemorrhage].
OBJECTIVE
To investigate the changes in the expression of neuropeptide Y mRNA in the cerebral tissues of rat models of subarachnoid hemorrhage (SAH), to shed light on the prevention and treatment of cerebral vasospasm induced by SAH.


METHODS
SAH rat models, saline and sham-operation control groups were respectively established, in which the relative abundance of neuropeptide Y mRNA in the corpus striatum, cerebral cortex, hypothalamus were subsequently measured and compared by reverse transcriptase PCR at 15 min, 30 min and 1, 4, 8, 12, 24, 48 h after corresponding treatment.


RESULTS
The abundance of neuropeptide Y mRNA significantly elevated in the cerebral tissues after SAH, and gradually declined with the passage of time, but were still significantly higher than that of saline and sham-operation controls at any of the same time points.


CONCLUSIONS
Neuropeptide Y mRNA level in the cerebral tissues significantly elevates after SAH, which may play an important role in inducing cerebral vasospasm.
Lin
Jing-ming
JM
Department of Pharmaceutics, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Yang
Wei-mong
WM
Yang
Jin-xing
JX
Xia
Ping-guang
PG
D000818:Animals; D001921:Brain / Q000378:metabolism*; D008297:Male; D009478:Neuropeptide Y / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013345:Subarachnoid Hemorrhage / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D020301:Vasospasm, Intracranial / Q000209:etiology / Q000235:genetics / Q000378:metabolism*
D009478:Neuropeptide Y; D012333:RNA, Messenger