Flux control of the bacterial phosphoenolpyruvate:glucose phosphotransferase system and the effect of diffusion.
We analyzed the role of diffusion and cell size on the flux control properties of the glucose-PTS of Escherichia coli, in silicon cells under various metabolic conditions. To our surprise, the influence of the concentration of phosphoryl-donor PEP on the distribution of control was small. We found for cells of bacterial size that PTS-flux control was mainly located in processes taking place in the membrane and that diffusion hardly controlled the flux (< 2.8 %). Enlargement of the cells shifted the control from membrane to cytoplasm and from process rates to diffusion rates, the latter now having a total control of about 38 %. In the presence of glucose, nearly all diffusion flux control resided in the component that links the cytoplasmic processes to those in the membrane.
Francke
Christof
C
BioCentrum Amsterdam, Dept of Molecular Cell Physiology, Free University. cfrancke@bio.vu.nl
Westerhoff
Hans V
HV
Blom
Joke G
JG
Peletier
Mark A
MA
D004058:Diffusion; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008433:Mathematics; D008954:Models, Biological; D010728:Phosphoenolpyruvate / Q000378:metabolism*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*
D029968:Escherichia coli Proteins; D010728:Phosphoenolpyruvate; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase; D005947:Glucose