title: Alteration of cell morphology and viability in a recA mutant of Streptococcus thermophilus upon induction of heat shock and nutrient starvation.
abstract: We identified the recA gene of the moderately thermophilic bacterium Streptococcus thermophilus and investigated the role of its product in the adaptation to heat shock and nutrient starvation. Expression of recA was required for optimal viability and normal cell morphology upon induction of both stresses. Normal induction of GroEL and ClpL in a recA knock-out mutant suggests that the RecA role in heat shock and nutrient starvation response of S. thermophilus is independent from the intracellular accumulation of these stress-specific chaperones.
authors.affiliation: Dipartimento di Fisiologia Generale ed Ambientale, Università Federico II, via Mezzocannone 16, 80134 Naples, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Giliberti
authors.forename: Gino
authors.initials: G
authors.lastname: Naclerio
authors.forename: Luca
authors.initials: L
authors.lastname: Martirani
authors.forename: Ezio
authors.initials: E
authors.lastname: Ricca
authors.forename: Maurilio
authors.initials: M
authors.lastname: De Felice
mesh_terms: D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D018834:Chaperonin 60 / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004917:Erythromycin / Q000494:pharmacology; D006358:Hot Temperature; D008855:Microscopy, Electron, Scanning; D018832:Molecular Chaperones / Q000378:metabolism; D009154:Mutation; D011938:Rec A Recombinases / Q000235:genetics*; D013291:Streptococcus / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D018832:Molecular Chaperones; D004917:Erythromycin; D011938:Rec A Recombinases