Marsupialization inhibits interleukin-1alpha expression and epithelial cell proliferation in odontogenic keratocysts.
BACKGROUND
Marsupialization results in the reduction of odontogenic cyst size. Interleukin-1alpha (IL-1alpha) is thought to play a crucial role for the expansion of odontogenic keratocysts. The aim of this study was to investigate the effects of marsupialization on the expression of IL-1alpha and on the proliferating activity of a lining epithelium in odontogenic keratocysts.


METHODS
The concentrations of IL-1alpha, interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in the intracystic fluids of odontogenic keratocysts were measured by the enzyme-linked immunosorbent assay (ELISA). The expression of IL-1alpha mRNA in odontogenic keratocysts was measured before and after marsupialization by in situ hybridization. The expression of IL-1alpha and epithelial cell-proliferating activities in odontogenic keratocysts were also measured by immunohistochemistry using antibodies for human IL-1alpha and Ki-67 antigen, respectively.


RESULTS
The intracystic fluid levels of IL-1alpha were significantly higher than those of IL-6 and TNF-alpha in odontogenic keratocysts. In situ hybridization and immunohistochemistry showed that strong expression of IL-1alpha mRNA and protein was mainly detected in the epithelial cells of odontogenic keratocysts. After marsupialization, the signal intensities for IL-1alpha mRNA and protein were significantly decreased. In addition, the Ki-67 labeling index of the epithelial cells was decreased proportionally with the grade of IL-1alpha mRNA expression after the marsupialization.


CONCLUSIONS
Our findings suggest that marsupialization may reduce the size of odontogenic keratocyst by inhibiting IL-1alpha expression and the epithelial cell proliferation.
Ninomiya
Tomohiro
T
Department of Oral and Maxillofacial Surgery, Graduate School of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
Kubota
Yasutaka
Y
Koji
Takehiko
T
Shirasuna
Kanemitsu
K
D000328:Adult; D002455:Cell Division; D016009:Chi-Square Distribution; D020420:Cyst Fluid / Q000737:chemistry* / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D009807:Odontogenic Cysts / Q000378:metabolism* / Q000601:surgery*; D015345:Oligonucleotide Probes; D019647:Oral Surgical Procedures; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis
D007375:Interleukin-1; D015850:Interleukin-6; D019394:Ki-67 Antigen; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha