[Studies on the stability of sodium taurocholate in different media and in the course of sterilization].
OBJECTIVE
To investigate the stability of the main active constituent of snake gall sodium taurocholate, TCANa in 25% alcoholic solution and artificial gastric juice, and in the course of hot pressurized sterilization.


METHODS
The stability of the solution was predicted by an accelerated isothermal test, and the content of TCANa was determined by TLC-colorimetry.


RESULTS
The half-time(t1/2) of TCANa in 25% alcoholic solution is 60.57 d at 25 degrees C, and in artificial gastric juice 11.37 d at 37 degrees C, the loss rate under the condition(115 degrees C, 30 min) of sterilization about 3%.


CONCLUSIONS
TCANa in 25% alcoholic solution is unstable. Therefore it is suggested that in order to raise the stability of TCANa the alcoholic concentration be increased and the solution be stored at temperatures below 0 degree C.
Liu
Y
Y
School of Pharmacy, Tongji Medical University, Wuhan 430030.
Shi
C
C
Hu
Y
Y
Zheng
Z
Z
Lu
X
X
D000818:Animals; D004355:Drug Stability; D005704:Gallbladder / Q000737:chemistry*; D005750:Gastric Juice; D008419:Materia Medica / Q000737:chemistry*; D012911:Snakes; D013242:Sterilization; D013656:Taurocholic Acid / Q000302:isolation & purification*
D008419:Materia Medica; D013656:Taurocholic Acid