Mutation of ras oncogene in di-isopropanolnitrosamine-induced rat thyroid carcinogenesis.
To clarify the role of ras gene mutation in thyroid tumorigenesis, DNAs extracted from various rat thyroid lesions induced by di-isopropanolnitrosamine (DIPN) administration were analyzed using the microdissection-polymerase chain reaction-direct sequence (MD-PCR-DS) method. The MD-PCR-DS method revealed that K- ras gene mutation (G-A transition at codon 12) was frequently detected in nodular lesions (incidence of mutation 75%) and absent in diffuse hyperplastic and pre-nodular lesions. Although the incidence of mutation in nodular lesions was not correlated with the histological type (type 2A 76%; type 2B 84%; and type 3 71%) or treatment period (15 weeks 84% and 30 weeks 71%), it was correlated with the administration method (single injection 55% and serial injection 91%). In conclusion, K- ras mutation plays an important role in DIPN-induced rat thyroid tumorigenesis, possibly regarded as an early event in the tumorigenic process.
Kobayashi
Yoshihiko
Y
Fuji Biomedix Co., Ltd. Kobuchizawa Laboratories 10221, Kobuchizawa-cho, Kitakoma-gun, Yamanashi-ken, 408-0044, Japan.
Kawaoi
Akira
A
Katoh
Ryohei
R
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D011905:Genes, ras; D008297:Male; D009154:Mutation; D009602:Nitrosamines / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013964:Thyroid Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology
D002273:Carcinogens; D009602:Nitrosamines; C012457:diisopropanolnitrosamine