Effect of different particles on cell proliferation in polymer scaffolds using a solvent-casting and particulate leaching technique.
Solvent-casting and particulate leaching are widely used in the manufacturing of porous polymer scaffolds. Salt is the most commonly used particulate because it is easily available and very easy to handle. Gelatin particles are another candidate for this method because they are known as a material that enhances cell attachment and proliferation. In this study, we compared the biocompatibility of the two scaffolds made from either salt (salt scaffold) or gelatin particles (gelatin scaffold). Sieved particles of salt and gelatin (particle size ranging 100-180 um) were dispersed in a poly-lactic-co-glycolic acid (PLGA)/chloroform solution and cast in a Teflon container. The solvent was allowed to evaporate and residual amounts were removed by vacuum drying. The particles were allowed to leach out by immersion in warm water (40 degrees C). Cultured chondrocytes (from knee cartilage) and smooth muscle cells (from bladder) were seeded on each scaffolds (5 x 10(6)/cm2) and cultured for 3 weeks, and their proliferation was compared using hematoxylin and eosin staining. These results demonstrated that the gelatin scaffold showed better attachment of cells at the initial stage, and both cell types showed much better proliferation of cells during 3 months. The better performance of a gelatin scaffold also contributed to the better connection of pores at the same porosity.
Suh
Soo Won
SW
Department of Biomedical Engineering, Samsung Medical Center, School of Medicine, Sungkyunkwan University, Seoul, Korea.
Shin
Ji Youn
JY
Kim
Jinhoon
J
Kim
Jhingook
J
Beak
Chung Hwan
CH
Kim
Dong-Ik
DI
Kim
Hojoong
H
Jeon
Seong Soo
SS
Choo
In-Wook
IW
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002455:Cell Division; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D004396:Coloring Agents; D004285:Dogs; D005780:Gelatin; D032342:Muscle Cells / Q000166:cytology*; D009130:Muscle, Smooth / Q000166:cytology; D010078:Oxazines; D011108:Polymers / Q000737:chemistry*; D011161:Porifera; D012492:Salts; D012997:Solvents; D023822:Tissue Engineering / Q000379:methods; D014966:Xanthenes
D001672:Biocompatible Materials; D004396:Coloring Agents; D010078:Oxazines; D011108:Polymers; D012492:Salts; D012997:Solvents; D014966:Xanthenes; C005843:resazurin; D005780:Gelatin