Influence of interleukin 10 and transforming growth factor-beta on T cell stimulation through allogeneic retinal pigment epithelium cells in vitro.
The effect of interleukin 10 (IL-10) and transforming growth factor-beta (TGF-beta) on the immunological capability of human retinal pigment epithelium (RPE) cells was tested by measuring the proliferation of allogeneic T cells after stimulation through the RPE cells in vitro. For this, human RPE cells were activated with different concentrations of interferon-gamma (0, 50, 100 and 250 U/ml) used as stimulator cells. The following T cell proliferation was measured with a tetrazolium-based colorimetric assay over a period of up to 4 days. Our results show that RPE cells are able to stimulate allogeneic T cells in the presence or absence of professional antigen-presenting cells (macrophages). Additionally, the proliferation could be significantly decreased by IL-10 or TGF-beta. The maximum of suppression, especially by TGF-beta, was found after 48 and 72 h of incubation. This shows that the antigen-presenting properties of human RPE cells can be altered in culture.
Enzmann
Volker
V
Department of Ophthalmology and Visual Sciences, School of Medicine, University of Louisville, Louisville, Ky., USA.
Hollborn
Margrit
M
Kühnhoff
Sandra
S
Wiedemann
Peter
P
Kohen
Leon
L
D000938:Antigen-Presenting Cells / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D016212:Transforming Growth Factor beta; D016753:Interleukin-10