[Determination of gallic acid in garden burnet by HPLC].
OBJECTIVE
To establish a HPLC method for the determination of gallic acid in garden burnet.


METHODS
The sample was extracted with methanol under ultrasonic management for 25 min. Chromatographic conditions: Shimadzu LC-6A with SPD-6AV detector at 270 nm and auto injector SIL-6A, methanol-0.025 mol/L phosphoric acid(15:85) as the mobile phase. Shin-pack CLC-ODS column(6.0 mm x 15 mm).


RESULTS
Gallic acid in garden burnet was baseline separated and determined. The average content was 0.22%, recovery rate 97.4% and relative standard deviation 1.3%.


CONCLUSIONS
Simple, rapid and accurate, the method provides new indexes for quality control of Garden Burnet.
Sha
M
M
Liaoning College of Traditional Chinese Medicine, Shenyang 110032.
Cao
A
A
Wang
B
B
Liu
C
C
Yang
S
S
D002851:Chromatography, High Pressure Liquid; D005707:Gallic Acid / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D019685:Rosales / Q000737:chemistry*
D005707:Gallic Acid