title: Macrolide resistance phenotypes in Streptococcus pneumoniae in Santiago, Chile.
abstract: The mechanism of resistance was investigated in 39 macrolide-resistant clinical isolates of Streptococcus pneumoniae isolated from January 1997 to July 1999 in Santiago, Chile. Our results showed that 22 (56.5%) were macrolide-resistant, clindamycin-susceptible isolates (M phenotype) and 17 (43.5%) were macrolide and clindamycin resistant (MLS(B) phenotype). mefE gene was detected in all M phenotype, while ermB gene was detected in all MLS(B)-phenotype strains. Serotype 14 was the most frequent serotype among M-phenotype strains, and serotypes 19 and 23F were the most frequent serotypes in MLS(B) strains. These results demonstrate that both phenotypes of macrolide-resistant S. pneumoniae are found in Santiago, Chile, with the M phenotype predominating.
authors.affiliation: Laboratory Services, Universidad Catolica, Santiago, Chile. epalavecino@hotmail.com
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Palavecino
authors.forename: I
authors.initials: I
authors.lastname: Riedel
authors.forename: C
authors.initials: C
authors.lastname: Duran
authors.forename: S
authors.initials: S
authors.lastname: Bajaksouzian
authors.forename: M
authors.initials: M
authors.lastname: Joloba
authors.forename: T
authors.initials: T
authors.lastname: Davies
authors.forename: P C
authors.initials: PC
authors.lastname: Appelbaum
authors.forename: M R
authors.initials: MR
authors.lastname: Jacobs
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D002648:Child; D002677:Chile; D002981:Clindamycin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology*; D006801:Humans; D008565:Membrane Proteins; D008780:Methyltransferases / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification / Q000187:drug effects* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C431304:MefE protein, Streptococcus pneumoniae; D008565:Membrane Proteins; D002981:Clindamycin; D004917:Erythromycin; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase