Derivatization of small biomolecules for optimized matrix-assisted laser desorption/ionization mass spectrometry.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is a powerful tool for the measurement of low molecular mass compounds of biological interest. The limitations for this method are the volatility of many analytes, possible interference with matrix signals or bad ionization or desorption behavior of the compounds. We investigated the application of well-known and straightforward one-pot derivatization procedures to circumvent these problems. The derivatizations tested allow the measurement and the labeling of alcohols, aldehydes and ketones, carboxylic acids, alpha-ketocarboxylic acids and amines.
Tholey
Andreas
A
Technische Biochemie, Universität des Saarlandes, 66123 Saarbrücken, Germany.
Wittmann
Christoph
C
Kang
Min-Jung
MJ
Bungert
Ditte
D
Hollemeyer
Klaus
K
Heinzle
Elmar
E
D000438:Alcohols / Q000737:chemistry; D000447:Aldehydes / Q000737:chemistry; D000588:Amines / Q000737:chemistry; D001704:Biopolymers / Q000032:analysis*; D002264:Carboxylic Acids / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D007202:Indicators and Reagents; D007659:Ketones / Q000737:chemistry; D008970:Molecular Weight; D012997:Solvents; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D000438:Alcohols; D000447:Aldehydes; D000588:Amines; D001704:Biopolymers; D002264:Carboxylic Acids; D005227:Fatty Acids; D007202:Indicators and Reagents; D007659:Ketones; D012997:Solvents