Recombinant glucocorticoid induced tumour necrosis factor receptor (rGITR) induced COX-2 activity in murine macrophage Raw 264.7 cells.
Stimulation of murine macrophages with recombinant soluble glucocorticoid induced tumour necrosis factor receptor (GITR) induced cyclooxygenase (COX)-2 protein and generated significant amounts of PGE(2). Previous result demonstrated that macrophages express GITR and GITR ligand constitutively. Induction of COX-2 was synergistic with interferon (INF)-gamma. GITR/ligand system could deliver an activation signal to macrophages in inflammatory processes.
Shin
Hyun-Hee
HH
Department of Biological Sciences and the Immunomodulation Research Center, University of Ulsan, Ulsan, Korea.
Kwon
Byoung S
BS
Choi
Hye-Seon
HS
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D051546:Cyclooxygenase 2; D018076:DNA, Complementary / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D053450:Glucocorticoid-Induced TNFR-Related Protein; D015151:Immunoblotting; D007371:Interferon-gamma / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008024:Ligands; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013997:Time Factors
D018076:DNA, Complementary; D053450:Glucocorticoid-Induced TNFR-Related Protein; D007527:Isoenzymes; D008024:Ligands; D017475:Receptors, Nerve Growth Factor; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; C106544:Tnfrsf18 protein, mouse; D007371:Interferon-gamma; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone