Cytogenetic biomonitoring of workers from laboratories of clinical analyses occupationally exposed to chemicals.
A cytogenetic monitoring study was carried out on a group of workers in clinical analysis laboratories to investigate the risk of occupational exposure to chronic low levels of chemicals.Thirty-four clinical laboratories have been involved in the study. In these laboratories, toxicants and analytical procedures utilized have been characterized. The individual occupational exposure of workers was assessed by use of a questionnaire concerning the chemical substances utilized. About 300 different chemicals have been identified. Cytogenetic analyses (chromosomal aberration and micronucleus tests) were carried out on a strictly selected group of 50 workers enrolled from these laboratories and compared to 53 controls (healthy blood donors) matched for gender and age. The exposed group shows a significantly higher frequency of genetic damage than the control group. Both chromatid and chromosome aberration frequencies in workers appear significantly higher than in controls. Similarly, comparison between micronucleated cells rates of exposed and unexposed groups show significantly higher frequencies of binucleated cells with micronucleus (BNMN) and of total micronuclei (MN tot) in workers than in controls.
Testa
A
A
Section of Toxicology and Biomedical Science, E.N.E.A, CR Casaccia, Via Anguillarese 301, 00060, Rome, Italy. antonella.testa@casaccia.enea.it
Ranaldi
R
R
Carpineto
L
L
Pacchierotti
F
F
Tirindelli
D
D
Fabiani
L
L
Giuliani
A R
AR
Urso
M
M
Rossini
A
A
Materazzo
F
F
Petyx
M
M
Leoni
V
V
D000328:Adult; D016022:Case-Control Studies; D002455:Cell Division / Q000502:physiology; D002869:Chromosome Aberrations / Q000187:drug effects*; D004784:Environmental Monitoring; D005260:Female; D006801:Humans; D007621:Karyotyping; D007755:Laboratories, Hospital; D008214:Lymphocytes / Q000097:blood / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D010564:Personnel, Hospital