title: [Changes in serum concentration of podophyllotoxin after its topical application with liposome on rat skin].
abstract: OBJECTIVE
  To investigate the changes of serum concentration of podophyllotoxin after topical application of liposome podophyllotoxin suspension on rat skin.
  
  
  METHODS
  SD rats were used in this study, which were divided into test group (n=48) to receive application of liposome podophyllotoxin (0.5 % ) suspension and control group (n=48) treated with 0.5 % podophyllotoxin alcohol solution. Blood samples were obtained from the heart at 1, 2, 4, 6, 8, 10, 12 and 24 h respectively after drug application, and the serum concentration of podophyllotoxin was determined by spectrofluorometry.
  
  
  RESULTS
  The area under the curve of plasma drug concentration of the control group was 2.3-fold greater than that of the test group. Eight hours after drug application, the serum concentration of podophyllotoxin reached the peak in the test group, while in the control group, only two hours was needed to reach the peak. The peak serum level of podophyllotoxin in the test group were significantly lower than that of the control group (166.395 +/- 14.634 ng/ml vs 378.603 +/- 26.105 ng/ml, P<0.001).
  
  
  CONCLUSIONS
  The systemic absorption of podophyllotoxin in rats after its topical application in liposome suspension is significantly lower than that after application of 0.5 % podophyllotoxin alcohol solution, therefore the systemic toxicity may be reduced.
authors.affiliation: Department of Dermatology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: San-quan
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Kang
authors.initials: K
authors.lastname: Zeng
authors.forename: Bin-bin
authors.initials: BB
authors.lastname: Jiang
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Li
authors.forename: Yu-sheng
authors.initials: YS
authors.lastname: Xie
authors.forename: Xiao-liang
authors.initials: XL
authors.lastname: Zhu
authors.forename: Guo-feng
authors.initials: GF
authors.lastname: Li
mesh_terms: D000279:Administration, Cutaneous; D000465:Algorithms; D000818:Animals; D019540:Area Under Curve; D005260:Female; D008081:Liposomes; D008297:Male; D011034:Podophyllotoxin / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D013050:Spectrometry, Fluorescence
chemical_list: D008081:Liposomes; D011034:Podophyllotoxin