Caveolin expression during chondrogenesis in the avian limb.
Caveolin is the principal component and critical structural and functional element of caveolae, omega-shaped plasmalemmal invaginations, which have been implicated in a wide range of cellular processes in several different tissues. In the present study, we have investigated both the spatial and temporal expression of caveolin proteins during chondrogenesis in the avian tibiotarsus at days 10-20 of embryonic development. By using semiquantitative Western blotting, we found that caveolin-1 was clearly expressed in developing avian cartilage. The positive expression of caveolin-1 in cartilage showed an upward trend of accumulation temporally, with the highest levels of expression at 20 days of development. By using immunocytochemistry, we detected all three caveolin proteins in the cells of the outer fibrous articular surface, although caveolin-1 demonstrated the strongest and most consistent reactivity. In all cases, however, immunoreactivity appeared to be concentrated in cells facing the articular cavity. In the epiphyseal cartilage, immunocytochemistry revealed that caveolin-1 was present in the majority of chondrocytes within all layers of the cartilage and at all stages examined. A discrete, intense band of caveolin-1 immunoreactivity was apparent within the layer of flattened cells immediately underlying the proliferating rounded chondrocytes and suggests that caveolin-1 might be involved in regulating the progression of cells through these gradually maturing cell layers. In contrast to the results for caveolin-1, in the case of caveolin-2 and -3, chondrocytes were devoid of immunoreactivity in all regions of the epiphyseal cartilage. Overall, this study demonstrates that caveolin-1, -2, and -3 are expressed during chondrogenesis in the developing avian limb, although the patterns of expression are restricted both spatially and temporally throughout the differentiating cell layers of the cartilage. The results suggest that caveolin proteins might play a differentiation-dependent role during avian chondrogenesis.
Hollins
Andrew J
AJ
Pharmaceutical Cell Biology, Welsh School of Pharmacy, Cardiff University, Wales, United Kingdom.
Campbell
Lee
L
Gumbleton
Mark
M
Evans
Darrell J R
DJ
D000818:Animals; D015153:Blotting, Western; D002358:Cartilage, Articular / Q000196:embryology; D051242:Caveolin 1; D022461:Caveolins / Q000096:biosynthesis*; D002454:Cell Differentiation; D002642:Chick Embryo; D019902:Chondrocytes / Q000378:metabolism*; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry
D051242:Caveolin 1; D022461:Caveolins