[HPLC determination of uracil xanthine, hypoxanthine and uridine in species of animal drugs].
OBJECTIVE
To make further studies on the water-soluble components in animal drugs by determining the contents of uracil, xanthine, hypoxanthine and uridine from ten species of animal drugs.


METHODS
The determination was affected by RP-HPLC, using Shim-pack CLC-ODS column(150 mm x 6 mm), 0.05 mol/L dibasic ammonium phosphate solution as mobile phase, flow rate 0.8 ml/min and detection at 254 nm.


RESULTS
This method is accurate, rapid and reproducible. Analytical data for Hirudo nipponia, Eupolyphaga sinensis, Scolopendra subspinipes mutilans, Buthus martensii, Bombyx mori, Mylabris cicorri, Aspongopus chinensis, Lytta caraganae, Tabanus bivittatus and Huechys sanguinea are given.


CONCLUSIONS
There are marked discrepancies in the contents of the four components as shown on the chromatograms, a point that may be helpful in identifying the above-cited ten species of animal drugs.
Liu
L
L
Department of Chinese Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210038.
Jin
R
R
Xu
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019271:Hypoxanthine / Q000032:analysis*; D007313:Insecta / Q000737:chemistry; D008419:Materia Medica / Q000737:chemistry*; D014498:Uracil / Q000032:analysis*; D014529:Uridine / Q000032:analysis*; D019820:Xanthine / Q000032:analysis*
D008419:Materia Medica; D019820:Xanthine; D019271:Hypoxanthine; D014498:Uracil; D014529:Uridine