title: [Spin-spin interaction upon introduction of a spin label into immunoglobulins M and G at the carbohydrate moiety].
abstract: By spin labeling the monoclonal IgM and normal IgG at the carbohydrate moiety with 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl, preparations were obtained whose ESR spectra indicate rapid exchange spin-spin interactions between two spin labels. It was shown that, in the case of spin-labeled IgM, this spectrum is determined by a glycopeptide noncovalently bound to IgM, which incorporates two spin labels.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 117984 Russia.
authors.forename: V P
authors.initials: VP
authors.lastname: Timofeev
authors.forename: D O
authors.initials: DO
authors.lastname: Nikol'skiĭ
authors.forename: V A
authors.initials: VA
authors.lastname: Lapuk
authors.forename: V A
authors.initials: VA
authors.lastname: Aleshkin
mesh_terms: D002850:Chromatography, Gel; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D007074:Immunoglobulin G / Q000737:chemistry*; D007075:Immunoglobulin M / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D003497:Cyclic N-Oxides; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009844:Oligosaccharides; D013113:Spin Labels; C015527:4-amino-TEMPO