Ratiometric probes for hydrogencarbonate analysis in intracellular or extracellular environments using europium luminescence.
A series of six, cationic, zwitterionic and anionic Eu complexes has been examined for the analysis of hydrogencarbonate concentration in the intracellular and extracellular ranges; an anionic complex incorporating three glutarate residues and a sensitising acridone chromophore (lambda exc = 410 nm) exhibits a 69% change in the intensity ratio of the 618/588 nm Eu emission bands between 5 and 15 mM HCO3- in a cell lysate medium.
Bretonnière
Yann
Y
Department of Chemistry, University of Durham, South Road, Durham, UK DH1 3LE.
Cann
Martin J
MJ
Parker
David
D
Slater
Rachel
R