A new pentaplex system to study short tandem repeat markers of forensic interest on X chromosome.
A new method has been optimised to amplify five X chromosome short tandem repeat (STR) markers of interest in forensic medicine: human phosphoribosyl transferase (HPRTB), DXS101, androgen receptor (ARA), DXS7423 and DXS8377. Markers were conveniently amplified in a single PCR reaction with fluorochrome-labelled primers, which allowed the analysis of fragment sizes after injection into a capillary electrophoresis system. The most common alleles of each locus were sequenced and used in a control ladder to type unknown samples.
Zarrabeitia
María T
MT
Unit of Legal Medicine, Faculty of Medicine, University of Cantabria, Av. Herrera Oria, s/n 30911 Santander, Spain. zarrabet@unican.es
Amigo
Teresa
T
Sañudo
Carolina
C
Zarrabeitia
Ana
A
González-Lamuño
Domingo
D
Riancho
José A
JA
D000328:Adult; D041321:Chromosomes, Human, X; D019075:Electrophoresis, Capillary; D005554:Forensic Medicine / Q000379:methods*; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences
D005819:Genetic Markers