title: Leptin receptor isoform expression in rat osteoblasts and their functional analysis.
abstract: The genetic defect in producing the adipose hormone leptin results among others in a drastic increase of bone mass. The current understanding is that under normal circumstances, osteoblast activity is indirectly suppressed by a hypothalamic relay induced by leptin-signalling in the brain. To investigate whether leptin might also regulate osteoblast activity in a direct manner, expression of leptin receptors in rat osteoblasts was determined and their functionality was analyzed upon recombinant leptin treatment. Reverse transcription-PCR confirmed the expression of four among the six currently described receptor isoforms, which were also able to transduce cell signalling as shown by STAT3 phosphorylation after activation.
authors.affiliation: Laboratory for the Study of Molecular Biointerface, Department of Oral Anatomy, College of Dentistry and Intellectual Biointerface Engineering Center (IBEC), BK21 HLS, Seoul National University, Yeonkun-Dong, Chongro-Ku, South Korea.
authors.forename: Yun-Jung
authors.initials: YJ
authors.lastname: Lee
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Sung-Kyu
authors.initials: SK
authors.lastname: Ju
authors.forename: Kwan-Hee
authors.initials: KH
authors.lastname: You
authors.forename: Jea Seung
authors.initials: JS
authors.lastname: Ko
authors.forename: Hyun-Man
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism; D010006:Osteoblasts / Q000502:physiology*; D010766:Phosphorylation; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D054411:Receptors, Leptin; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D015534:Trans-Activators