[Determination of alkaloids in radix Sophorae flavescentis by high performance capillary electrophoresis].
OBJECTIVE
To develop a simple and rapid method for the determination of alkaloids in Radix Sophorae Flavescentis (RSF).


METHODS
HPCE buffer: 20 mmol.L-1 phosphates, pH 7.0; V = 20 kV, leff = 50 cm, L = 64.5 cm, i.d. = 50 microns, lambda = 201 nm, T = 25 degrees C, sample size = 10 kPa.s.


RESULTS
Matrine(I) and oxymatrine(II) in RSF were completely separated with recoveries of 97.47% and 97.67% respectively.


CONCLUSIONS
Simple, accurate and reproducible, this method can be used for the quality control of Chinese herbal medicines.
Li
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Hunan Academy of Traditional Chinese Medicine and Materia Medica, Changsha 410013.
Wang
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D000470:Alkaloids / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D011807:Quinolizines; D029909:Sophora / Q000737:chemistry*; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; C037573:oxymatrine; D000093842:Matrines