Development of (17)O NMR approach for fast imaging of cerebral metabolic rate of oxygen in rat brain at high field.
A comprehensive technique was developed for using three-dimensional (17)O magnetic resonance spectroscopic imaging at 9.4T for rapidly imaging the cerebral metabolic rate of oxygen consumption (CMRO(2)) in the rat brain during a two-min inhalation of (17)O(2). The CMRO(2) value (2.19 +/- 0.14 micromol/g/min, n = 7) was determined in the rat anesthetized with alpha-chloralose by independent and concurrent (17)O NMR measurements of cerebral H(2)17O content, arterial input function, and cerebral perfusion. CMRO(2) values obtained were consistent with the literature results for similar conditions. Our results reveal that, because of its superior sensitivity at ultra-high fields, the (17)O magnetic resonance spectroscopic imaging approach is capable of detecting small dynamic changes of metabolic H(2)17O during a short inhalation of (17)O(2) gas, and ultimately, for imaging CMRO(2) in the small rat brain. This study provides a crucial step toward the goal of developing a robust and noninvasive (17)O NMR approach for imaging CMRO(2) in animal and human brains that can be used for studying the central role of oxidative metabolism in brain function under normal and diseased conditions, as well as for understanding the mechanisms underlying functional MRI.
Zhu
Xiao-Hong
XH
Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota Medical School, 2021 6th Street SE, Minneapolis, MN 55455, USA.
Zhang
Yi
Y
Tian
Run-Xia
RX
Lei
Hao
H
Zhang
Nanyin
N
Zhang
Xiaoliang
X
Merkle
Hellmut
H
Ugurbil
Kamil
K
Chen
Wei
W
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D015233:Models, Statistical; D009462:Neurology / Q000379:methods; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D017207:Rats, Sprague-Dawley; D013687:Telencephalon / Q000378:metabolism*; D013997:Time Factors
D010100:Oxygen