Distinctive response of thyroid-infiltrating mononuclear cells to B cell activation through CD40 and interleukin-4 in Graves' patients.
The possible role of abnormal T cell-dependent B-cell activation in Graves' disease was investigated by comparing lymphocyte subset distribution and the production of soluble CD8 (sCD8), sCD23, IL-10 and IL-12 by peripheral blood cells (PBMC) and thyroid-infiltrating lymphocytes (TL) in vitro. In TL, the percentage of CD8(+) cells was slightly higher and the sCD8 concentration was significantly higher than in PBMC. The ratio CD23(+) cells to CD20(+) cells (activated B/pan B cells) was increased in TL compared to PBMC from Graves' or normal controls, although the percentage of CD20(+) cells was decreased. Compared to PBMC in Graves' disease, the relative ratio of IL-10 to IL-12 release (IL-10/IL-12) by unstimulated TL was increased, despite a lack of significant difference between PBMC and TL in mean values for either IL-10 or IL-12 secretion. Incubating PBMC with a combination of anti-CD40 monoclonal antibodies and interleukin-4 (IL-4) resulted in B cell activation, reflected in an increase in the sCD23 level in both controls and Graves' patients, but especially prominent in the latter. Stimulation with anti-CD40 antibody and IL-4 also decreased the percentage of CD8(+) cells in PBMC but not TL from both Graves' disease and normal controls, and the percentage of CD8(+) cells in TL was higher than PBMC after the stimulation. The sCD23 concentration in TL was decreased compared to PBMC both in patients with Graves' disease and normal controls. However, in contrast to the increased responses observed in Graves' PBMC or normal controls after stimulation, sCD23 levels remained the same in stimulated TL from Graves' patients. This combination of B cell stimulants increased production of IL-10 in PBMC but not in TL obtained from patients with Graves' disease, and the increased IL-10/IL-12 ratio declined to a value no different from that in PBMC group after stimulation. Thus, T cell-dependent B-cell activation via a CD40 pathway may cause a shift in the Th(1)/Th(2) balance to Th(2) dominance in Graves' disease, while increased CD8(+) cells in TL may suppress sCD23 production and IL-10-producing Th(2) cells.
Itoh
Mitsuyasu
M
Department of Internal Medicine, Fujita Health University, School of Medicine, Aichi 470-1192, Japan. mituyasu@fujita-hu.ac.jp
Uchimura
Keiko
K
Yamamoto
Keiko
K
Makino
Masaki
M
Imamura
Shigeo
S
Kobayashi
Takako
T
Fujiwara
Kentaro
K
Kato
Taiya
T
Hayakawa
Nobuki
N
Sawai
Yoshikuni
Y
Nagasaka
Akio
A
Iwase
Katsumi
K
Nomura
Takahide
T
Hagino
Yasumichi
Y
D000328:Adult; D018951:Antigens, CD20 / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism*; D016827:CD8 Antigens / Q000096:biosynthesis; D002469:Cell Separation; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D006111:Graves Disease / Q000097:blood* / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D017455:Receptors, IgE / Q000096:biosynthesis; D018418:Th2 Cells; D013961:Thyroid Gland / Q000378:metabolism*
D018951:Antigens, CD20; D019013:CD40 Antigens; D016827:CD8 Antigens; D017455:Receptors, IgE; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4