NMR studies on the solution structure of a deletion mutant of the transcarboxylase biotin carrier subunit.
A deletion mutant of the transcarboxylase biotin carrier protein was completely labeled with 13C and 15N. A multitude of 2D and 3D NMR spectra were recorded and assigned. An NMR solution structure was derived from the data and compared in detail with the recently published structure of the wild-type. It is shown that deletion of 30% of the amino acids does not alter the structure of the rigid protein core.
Jank
Matthias M
MM
Institut für Analytische Chemie, Universität Leipzig, Linnestrasse 3, D-04103, Leipzig, Germany.
Sadowsky
Jack D
JD
Peikert
Claudia
C
Berger
Stefan
S
D000595:Amino Acid Sequence; D001710:Biotin / Q000378:metabolism*; D002247:Carbon Isotopes; D019878:Carboxyl and Carbamoyl Transferases / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D003196:Computer Graphics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009587:Nitrogen Isotopes; D017433:Protein Structure, Secondary; D021122:Protein Subunits / Q000737:chemistry; D011549:Pseudomonas / Q000201:enzymology; D011994:Recombinant Proteins / Q000737:chemistry; D017384:Sequence Deletion; D012996:Solutions
D002247:Carbon Isotopes; D002352:Carrier Proteins; D009587:Nitrogen Isotopes; D021122:Protein Subunits; D011994:Recombinant Proteins; D012996:Solutions; D001710:Biotin; D019878:Carboxyl and Carbamoyl Transferases; C021580:Methylmalonyl-CoA carboxytransferase