Identification of 1088 new transposon insertions of Caenorhabditis elegans: a pilot study toward large-scale screens.
We explored the feasibility of a strategy based on transposons to generate identified mutants of most Caenorhabditis elegans genes. A total of 1088 random new insertions of C. elegans transposons Tc1, Tc3, and Tc5 were identified by anchored PCR, some of which result in a mutant phenotype.
Martin
Edwige
E
CGMC, CNRS-UMR 5534, Université Lyon1, 69100 Villeurbanne, France.
Laloux
Hélène
H
Couette
Gaëlle
G
Alvarez
Thierry
T
Bessou
Catherine
C
Hauser
Oliver
O
Sookhareea
Satis
S
Labouesse
Michel
M
Ségalat
Laurent
L
D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D017931:DNA Primers; D004251:DNA Transposable Elements; D005091:Exons; D009153:Mutagens; D010865:Pilot Projects
D017931:DNA Primers; D004251:DNA Transposable Elements; D009153:Mutagens