title: Derivatization of small biomolecules for optimized matrix-assisted laser desorption/ionization mass spectrometry.
abstract: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is a powerful tool for the measurement of low molecular mass compounds of biological interest. The limitations for this method are the volatility of many analytes, possible interference with matrix signals or bad ionization or desorption behavior of the compounds. We investigated the application of well-known and straightforward one-pot derivatization procedures to circumvent these problems. The derivatizations tested allow the measurement and the labeling of alcohols, aldehydes and ketones, carboxylic acids, alpha-ketocarboxylic acids and amines.
authors.affiliation: Technische Biochemie, Universität des Saarlandes, 66123 Saarbrücken, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Tholey
authors.forename: Christoph
authors.initials: C
authors.lastname: Wittmann
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kang
authors.forename: Ditte
authors.initials: D
authors.lastname: Bungert
authors.forename: Klaus
authors.initials: K
authors.lastname: Hollemeyer
authors.forename: Elmar
authors.initials: E
authors.lastname: Heinzle
mesh_terms: D000438:Alcohols / Q000737:chemistry; D000447:Aldehydes / Q000737:chemistry; D000588:Amines / Q000737:chemistry; D001704:Biopolymers / Q000032:analysis*; D002264:Carboxylic Acids / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D007202:Indicators and Reagents; D007659:Ketones / Q000737:chemistry; D008970:Molecular Weight; D012997:Solvents; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000438:Alcohols; D000447:Aldehydes; D000588:Amines; D001704:Biopolymers; D002264:Carboxylic Acids; D005227:Fatty Acids; D007202:Indicators and Reagents; D007659:Ketones; D012997:Solvents