Top down analysis ceramide-induced mitochondrial dysfunctions: role of mitochondrial swelling.
Mitochondrial role in ceramide-induced apoptosis pathway remains unclear. Direct effects of ceramide on mitochondria (cytochrome c release, respiratory chain inhibition, oxygen radicals production...) have been reported [1, 2] and we previously showed that addition of ceramide to intact cells or isolated mitochondria triggers mitochondrial swelling which appeared to be insensitive to cyclosporin A (CsA) [3, 4]. The purpose of this work was to determine to which extent this CsA-insensitive mitochondrial swelling, therefore distinct from permeability transition, participates to ceramide-induced apoptosis. To achieve this, we applied Top-Down analysis of integrated mitochondrial function [5], in order to better understand ceramide-induced mitochondrial dysfunctions.
Leducq
Nathalie
N
Resonance Magnétique des Systémes Biologiques, UMR 5536, CNRS/Université Victor Segalen-Bordeaux 2, France.
Macouillard-Poulletier de Gannes
Florence
F
Rotureau
Marc-Antoine Belaud
MA
Belloc
Francis
F
Voisin
Pierre
P
Canioni
Paul
P
Diolez
Philippe
P
D000818:Animals; D017209:Apoptosis; D002518:Ceramides / Q000494:pharmacology*; D008564:Membrane Potentials / Q000502:physiology; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D008933:Mitochondrial Swelling; D015999:Multivariate Analysis; D010084:Oxidation-Reduction; D011522:Protons; D051381:Rats
D002518:Ceramides; D011522:Protons