Secretion of alpha-amylase from Pseudoalteromonas haloplanktis TAB23: two different pathways in different hosts.
Secretion of cold-adapted alpha-amylase from Pseudoalteromonas haloplanktis TAB23 was studied in three Antarctic bacteria. We demonstrated that the enzyme is specifically secreted in the psychrophilic hosts even in the absence of a protein domain that has been previously reported to be necessary for alpha-amylase secretion in Escherichia coli. The occurrence of two different secretion pathways in different hosts is proposed.
Tutino
Maria Luisa
ML
Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II--Complesso Universitario Monte Sant'Angelo, Naples, Italy. tutino@unina.it
Parrilli
Ermenegilda
E
Giaquinto
Laura
L
Duilio
Angela
A
Sannia
Giovanni
G
Feller
Georges
G
Marino
Gennaro
G
D000864:Antarctic Regions; D003080:Cold Temperature; D020563:Gammaproteobacteria / Q000201:enzymology* / Q000235:genetics; D005822:Genetic Vectors; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism*
D011994:Recombinant Proteins; D000516:alpha-Amylases