Urinary excretion of stress hormones of rats in tail-suspension.
Tail-suspension of rats has been employed for the study of disuse atrophy of hindlimb muscles and bones. From our study, it is suggested that stress exacerbates muscle and bone atrophy; however, the determination of stress hormones has been inappropriately performed. For assessing the effect of tail suspension on stress hormone secretion, suspension was performed for 7 days in a metabolic cage. Urinary excretions of corticosterone, catecholamines and antidiuretic hormone were determined during the 7-day suspension. Urine volume was unchanged during the suspension period, whereas water consumption was decreased during suspension. Urinary excretion of corticosterone and catecholamines increased significantly during the initial 3 days of suspension. The excretion of antidiuretic hormone increased throughout the suspension period. Our results demonstrated that the determination of urinary excretion of these hormones is useful for evaluating the stress reaction.
Kanda
K
K
Department of Endocrinology and Metabolism, Division of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya University, Nagoya, Japan.
Omori
S
S
Yamamoto
C
C
Miyamoto
N
N
Kawano
S
S
Murata
Y
Y
Matsui
N
N
Seo
H
H
D000311:Adrenal Glands / Q000502:physiology; D000818:Animals; D001835:Body Weight; D003345:Corticosterone / Q000378:metabolism* / Q000652:urine; D004837:Epinephrine / Q000378:metabolism* / Q000652:urine; D019417:Hindlimb Suspension; D008297:Male; D009133:Muscular Atrophy / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism* / Q000652:urine; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000378:metabolism* / Q000503:physiopathology / Q000652:urine; D013950:Thymus Gland / Q000502:physiology; D014556:Urine; D014667:Vasopressins / Q000378:metabolism* / Q000652:urine
D014667:Vasopressins; D003345:Corticosterone; D009638:Norepinephrine; D004837:Epinephrine