title: The screening of extracts from Goniothalamus scortechinii, Aralidium pinnatifidum and Andrographis paniculata for anti-malarial activity using the lactate dehydrogenase assay.
abstract: Goniothalamus scortechinii, Andrographis paniculata and Aralidium pinnatifidum were selected for the study based on their ethnomedicinal values. They were screened for anti-malarial activity towards Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The crude extract of G. scortechinii exhibited the most potent schizonticidal activity compared to the other extracts. It is effective against both the chloroquine resistant isolate, Gombak A and the sensitive strain, D10 of Plasmodium falciparum. Furthermore a better IC(50) value was obtained against the resistant strain, (9 microg/ml) compared to the sensitive strain, 40 microg/ml. When the crude extract was fractionated into 3 fractions, the chloroform fraction yielded the best activity, exhibiting equipotency against both strains of parasite used; IC(50) of 23.53 microg/ml against Gombak A and 21.06 microg/ml against D10.
authors.affiliation: Herbal Medicine Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia. siti_najila@hotmail.com
authors.forename: M J
authors.initials: MJ
authors.lastname: Siti Najila
authors.forename: A
authors.initials: A
authors.lastname: Noor Rain
authors.forename: A G
authors.initials: AG
authors.lastname: Mohamad Kamel
authors.forename: S I
authors.initials: SI
authors.lastname: Syed Zahir
authors.forename: S
authors.initials: S
authors.lastname: Khozirah
authors.forename: S
authors.initials: S
authors.lastname: Lokman Hakim
authors.forename: I
authors.initials: I
authors.lastname: Zakiah
authors.forename: A K
authors.initials: AK
authors.lastname: Azizol
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D035685:Andrographis; D000818:Animals; D029061:Annonaceae; D000962:Antimalarials / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001681:Biological Assay / Q000379:methods / Q000706:statistics & numerical data; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D018515:Plant Leaves; D018547:Plant Stems; D010963:Plasmodium falciparum / Q000187:drug effects
chemical_list: D000962:Antimalarials; D010936:Plant Extracts; D000445:Aldehyde Oxidoreductases; C021493:lactaldehyde dehydrogenase