How to distinguish between the vacuum cleaner and flippase mechanisms of the lmrA multi-drug transporter in Lactococcus lactis.
A numerical model of the LmrA multi-drug transport system of Lactococcus lactis is used to explore the possibility of distinguishing experimentally between two putative transport mechanisms, i.e., the vacuum-cleaner and the flippase mechanisms. This comparative model also serves as an example of numerical simulation with the scripting language Python and its scientific add-on Scipy.
Hofmeyr
J H S
JH
Dept of Biochemistry, University of Stellenbosch, South Africa. jhsh@sun.ac.za
Rohwer
J M
JM
Snoep
J L
JL
Westerhoff
H V
HV
Konings
W N
WN
D001426:Bacterial Proteins / Q000378:metabolism*; D003198:Computer Simulation; D018432:Drug Resistance, Multiple; D013294:Lactococcus lactis / Q000378:metabolism*; D008433:Mathematics; D008954:Models, Biological; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000378:metabolism*; D012984:Software
D001426:Bacterial Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C101824:LmrA protein, Lactococcus lactis