title: Novel locus required for expression of high-level macrolide-lincosamide-streptogramin B resistance in Staphylococcus aureus.
abstract: The yycF1(Ts) mutation in Staphylococcus aureus conferred hypersensitivity to macrolide-lincosamide-streptogramin B (MLS(B)) antibiotics on strains either containing or lacking ermB. The overexpression of the S. aureus Ssa protein restored the yycF1 mutant to wild-type levels of susceptibility. Inactivation of ssa in an unmutagenized strain dramatically reduced ermB-based resistance. Conditional loss of function or expression of ssa in the yycF1 mutant is proposed to result in the observed hypersensitivity to MLS(B) antibiotics.
authors.affiliation: Essential Therapeutics, Inc., Mountain View, California 94043, USA. pmartin@oselinc.com
authors.forename: P K
authors.initials: PK
authors.lastname: Martin
authors.forename: Y
authors.initials: Y
authors.lastname: Bao
authors.forename: E
authors.initials: E
authors.lastname: Boyer
authors.forename: K M
authors.initials: KM
authors.lastname: Winterberg
authors.forename: L
authors.initials: L
authors.lastname: McDowell
authors.forename: M B
authors.initials: MB
authors.lastname: Schmid
authors.forename: J M
authors.initials: JM
authors.lastname: Buysse
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial; D055231:Lincosamides; D018942:Macrolides; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D025361:Streptogramins / Q000494:pharmacology*; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D055231:Lincosamides; D018942:Macrolides; D025361:Streptogramins; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase