Changes in content of macrophage migration inhibitory factor secreted by Schwann cells after peripheral nerve injury.
OBJECTIVE
To investigate whether Schwann cells can secrete macrophage migration inhibitory factor (MIF) after peripheral nerve injury.


METHODS
Two kinds of infant rat Schwann cells(which were derived from intact and injured nerves respectively) were cultured in 10% newborn calf serum (NCS) DMEM/F12 medium for 72 h. Then the level of MIF in the conditioned media was determined by an enzyme-linked immunoadsordent assay (ELISA). As control,MIF level was also determined in 10% NCS DMEM/F12 medium without any cells.


RESULTS
The concentration of MIF in the conditioned medium of Schwann cells derived from injured nerves was significantly higher than that of control samples (P<0.05), while the concentration of MIF in the conditioned medium of Schwann cells from intact nerves was not elevated.


CONCLUSIONS
After peripheral nerve injury, Schwann cells can secrete MIF which may play an important role as an immunomodulatory cytokine for macrophage activation, inflammatory reactions and immune responses.
Huang
Tao
T
Institute of Clinical Anatomy, First Military Medical University, Guangzhou 510515, China.
Qin
Jian-Qiang
JQ
Huo
Xiao-Kun
XK
Yu
Lei
L
Xiong
Shao-Hu
SH
Liu
Da-Yong
DY
Zhong
Shi-Zhen
SZ
D000818:Animals; D005260:Female; D008263:Macrophage Migration-Inhibitory Factors / Q000378:metabolism*; D008297:Male; D059348:Peripheral Nerve Injuries; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000378:metabolism*
D008263:Macrophage Migration-Inhibitory Factors