[Determination of adenosine in Rehmannia glutinosa Libosch. by HPLC].
OBJECTIVE
To establish a method to determine the contents of asenosine in different species of Rehmannia glutinosa.


METHODS
The contents were determined by HPLC, with a column of KYWG-C18 as stationary phase, 6% ace tonitrile as mobile phase and detecting wavelength at 260 nm.


RESULTS
Adenosine has a good linearity in the range of 0.002 mg/ml-0.01 mg/ml, Y = 1.7742 x 10(-4) + 8.9021 x 10(-7) X, r = 0.9995. The average recovery is 94.1% and RSD = 1.86% (n = 5).


CONCLUSIONS
The method is fast, reliable and simple.
Zhu
Q
Q
Beijing Municipal Institute of Clinical Pharmacy Research, Beijing 100035.
Lou
Y
Y
Wang
Y
Y
Gong
T
T
Qie
C
C
D000241:Adenosine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D032267:Rehmannia / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D000241:Adenosine