title: A new pentaplex system to study short tandem repeat markers of forensic interest on X chromosome.
abstract: A new method has been optimised to amplify five X chromosome short tandem repeat (STR) markers of interest in forensic medicine: human phosphoribosyl transferase (HPRTB), DXS101, androgen receptor (ARA), DXS7423 and DXS8377. Markers were conveniently amplified in a single PCR reaction with fluorochrome-labelled primers, which allowed the analysis of fragment sizes after injection into a capillary electrophoresis system. The most common alleles of each locus were sequenced and used in a control ladder to type unknown samples.
authors.affiliation: Unit of Legal Medicine, Faculty of Medicine, University of Cantabria, Av. Herrera Oria, s/n 30911 Santander, Spain. zarrabet@unican.es
authors.forename: María T
authors.initials: MT
authors.lastname: Zarrabeitia
authors.forename: Teresa
authors.initials: T
authors.lastname: Amigo
authors.forename: Carolina
authors.initials: C
authors.lastname: Sañudo
authors.forename: Ana
authors.initials: A
authors.lastname: Zarrabeitia
authors.forename: Domingo
authors.initials: D
authors.lastname: González-Lamuño
authors.forename: José A
authors.initials: JA
authors.lastname: Riancho
mesh_terms: D000328:Adult; D041321:Chromosomes, Human, X; D019075:Electrophoresis, Capillary; D005554:Forensic Medicine / Q000379:methods*; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences
chemical_list: D005819:Genetic Markers