Effects of pre- and postnatal protein deprivation and postnatal refeeding on myenteric neurons of the rat large intestine: a quantitative morphological study.
We investigated the effect of protein deprivation and refeeding on weight gain, the size of the colon, and the numbers and sizes of enteric neurons. Neurons were located by reduced nicotinamide adenine dinucleotide (NADH) diaphorase staining. Protein deprivation of the mother throughout pregnancy, and the mother and unweaned rat pups in the first 21 postnatal days, reduced the weights of pups to about 50% of control. The size of the colon was also reduced, by about 40%. Despite this, total numbers of neurons in the colon were not reduced. However, there was a small, but significant, 15% reduction in the areas of neuron profiles. After 21 days the remaining pups were removed from the mothers, and either maintained on the control diet, maintained on the protein-deprived diet, or changed from the protein-deprived diet to a normal diet (refed group). These rats were examined after a further 21 days. Refeeding restored body weight to 20% below control, restored colon size, and restored nerve cell size. After a total of 42 days of protein deprivation, nerve cell numbers were not significantly different from control. In undernourished rats at 21 and 42 days, neurons were less well stained than control for NADH diaphorase. Refeeding between 21 and 42 days restored the normal appearance of the neurons. It is concluded that enteric neurons are protected from loss even when there is a substantial reduction in body weight and organ size caused by protein deprivation. The neurons become smaller, but recover size after refeeding.
Castelucci
Patrícia
P
Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Brazil. p.castelucci@unimelb.edu.au
de Souza
Romeu Rodrigues
RR
de Angelis
Rebeca Carlota
RC
Furness
John B
JB
Liberti
Edson Aparecido
EA
D000818:Animals; D048429:Cell Size / Q000502:physiology; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D007420:Intestine, Large / Q000196:embryology* / Q000254:growth & development* / Q000294:innervation; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology / Q000196:embryology* / Q000254:growth & development*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009929:Organ Size; D011247:Pregnancy; D039401:Prenatal Nutritional Physiological Phenomena / Q000502:physiology*; D011488:Protein Deficiency / Q000150:complications*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000502:physiology
D008058:Dihydrolipoamide Dehydrogenase