Evaluation of the anticonvulsant activity of the seed acetone extract of Ferula gummosa Boiss. against seizures induced by pentylenetetrazole and electroconvulsive shock in mice.
Ferula gummosa Boiss. (Apiaceae) which has been used as an antiepileptic remedy in Iranian traditional medicine was evaluated for anticonvulsant activity against experimental seizures. The seed acetone extract of F. gummosa protected mice against tonic convulsions induced by maximal electroshock (the median effective dose [ED(50)]=198.3 mg/kg) and especially by pentylenetetrazole (ED(50)=55 mg/kg). Neurotoxicity (sedation and motor impairment) of the extract was assessed by the rotarod test and the median toxic dose (TD(50)) value of 375.8 mg/kg was obtained. Preliminary phytochemical analysis showed the presence of terpenoids and alkaloids in the extract. The acceptable acute toxicity of the extract recommends further studies to determine the mechanism(s) and compound(s) involved in the anticonvulsant activity.
Sayyah
Mohammad
M
Department of Physiology and Pharmacology, Institute Pasteur of Iran, Tehran, Iran. sayyah@institute.pasteur.ac.ir
Mandgary
Ali
A
Kamalinejad
Mohammad
M
D000096:Acetone / Q000627:therapeutic use*; D000818:Animals; D000927:Anticonvulsants / Q000302:isolation & purification / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004597:Electroshock / Q000379:methods; D005309:Ferula; D008297:Male; D051379:Mice; D010433:Pentylenetetrazole / Q000633:toxicity*; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use; D012639:Seeds; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy*
D000927:Anticonvulsants; D010936:Plant Extracts; D000096:Acetone; D010433:Pentylenetetrazole