Enhanced cooperative binding of oligonucleotides to form DNA duplexes mediated by metal ion chelation.
Metal chelating iminodiacetic acid moieties were appended to abutting ends of two 9-mer oligonucleotides designed to hybridize contiguously on an 18-mer target. Chelation of 1 equivalent of Gd3+ cooperatively increases affinities of the oligonucleotides to the single stranded DNA target as evidenced by a 15 degrees C increase in Tm of the complex.
Horsey
Imogen
I
University Chemical Laboratories, University of Cambridge, Lensfield Road, Cambridge, UK CB2 1EW.
Krishnan-Ghosh
Yamuna
Y
Balasubramanian
Shankar
S
D000495:Allosteric Site; D020029:Base Pairing; D001483:Base Sequence; D002412:Cations / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D005682:Gadolinium / Q000737:chemistry*; D007098:Imino Acids / Q000737:chemistry; D015394:Molecular Structure; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
D002412:Cations; D002614:Chelating Agents; D004277:DNA, Single-Stranded; D007098:Imino Acids; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; D005682:Gadolinium; C008109:iminodiacetic acid