Mechanism and biological role of nitric oxide binding to cytochrome c'.
The binding of nitric oxide to ferric and ferrous Chromatium vinosum cytochrome c' was studied. The extinction coefficients for the ferric and ferrous nitric oxide complexes were measured. A binding model that included both a conformational change and dissociation of the dimer into subunits provided the best fit for the ferric cytochrome c' data. The NO (nitric oxide) binding affinity of the WT ferric form was found to be comparable to the affinities displayed by the ferric myoglobins and hemoglobins. Using an improved fitting model, positive cooperativity was found for the binding of NO to the WT ferric and ferrous forms, while anticooperativity was the case for the Y16F mutant. Structural explanations accounting for the binding are proposed. The NO affinity of ferrous cytochrome c' was found to be much lower than the affinities of myoglobins, hemoglobins, and pentacoordinate heme models. Structural factors accounting for the difference in affinities were analyzed. The NO affinity of ferrous cytochrome c' was found to be in the range typical of receptors and carriers. In addition, cytochrome c' was found to react with cytosolic light-irradiated membranes in the presence of succinate and carbon monoxide. With these results, a biochemical model of cytochrome c' functioning as a nitric oxide carrier was proposed.
Mayburd
Anatoly L
AL
Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, USA. amaybu@yahoo.com
Kassner
Richard J
RJ
D019943:Amino Acid Substitution / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D001665:Binding Sites / Q000235:genetics; D002844:Chromatium / Q000201:enzymology / Q000235:genetics; D003461:Crystallography; D003574:Cytochrome c Group / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D019281:Dimerization; D005290:Ferric Compounds / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry; D008956:Models, Chemical; D009569:Nitric Oxide / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000737:chemistry; D013053:Spectrophotometry; D013329:Structure-Activity Relationship
D001426:Bacterial Proteins; D003574:Cytochrome c Group; D005290:Ferric Compounds; D005296:Ferrous Compounds; D020030:Nitric Oxide Donors; D009569:Nitric Oxide