title: Histocompatible healing of periodontal defects after application of an injectable calcium phosphate bone cement. A preliminary study in dogs.
abstract: BACKGROUND
  A novel injectable, fast setting calcium phosphate cement (CPC) is currently used in orthopedic therapy for bone fractures. This study evaluated the possibility of applying this cement to healing periodontal defects.
  
  
  METHODS
  Fenestrations and 3-walled periodontal defects were surgically created on bilateral first molars and canines in 5 beagle dogs. CPC was applied to the defects on one side of the mandible. Untreated defects on the contralateral side served as controls. CPC was applied to all defects in the maxilla. Twelve weeks after surgery, the animals were sacrificed and decalcified and undecalcified specimens were prepared. Periodontal tissue healing was evaluated histologically and histometrically under a light microscope.
  
  
  RESULTS
  Healing of periodontal tissues in terms of bone and cementum formation was consistently observed in the CPC-applied sites. CPC was partly replaced by new bone. The residual CPC appeared detached from the denuded root surface. New cementum and periodontal ligament-like tissue were observed between the detached CPC and root surface. No unfavorable reaction was noted in the CPC-applied sites. No statistically significant difference was noted in the experimental or control sites under histometric analysis.
  
  
  CONCLUSIONS
  Although there were no statistically significant differences between the 2 treatment groups, histological observation indicated that CPC seemed to act as a scaffold for bone formation and provided histocompatible healing of periodontal tissues in this study. This cement might be applicable to periodontal therapy; however, further investigations are required.
authors.affiliation: Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Japan. yoshi.peri@tmd.ac.jp
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Shirakata
authors.forename: Shigeru
authors.initials: S
authors.lastname: Oda
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Kinoshita
authors.forename: Shigenari
authors.initials: S
authors.lastname: Kikuchi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tsuchioka
authors.forename: Isao
authors.initials: I
authors.lastname: Ishikawa
mesh_terms: D000539:Alveolar Process / Q000502:physiology; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D001843:Bone Cements / Q000737:chemistry / Q000627:therapeutic use*; D001861:Bone Regeneration; D002119:Calcium Carbonate / Q000008:administration & dosage / Q000627:therapeutic use; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000627:therapeutic use*; D003739:Dental Cementum / Q000502:physiology; D004285:Dogs; D007267:Injections; D008297:Male; D010510:Periodontal Diseases / Q000628:therapy*; D010710:Phosphates / Q000008:administration & dosage / Q000627:therapeutic use; D012038:Regeneration
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; D002130:Calcium Phosphates; D010710:Phosphates; D002119:Calcium Carbonate; C018279:sodium phosphate