Transcriptional activator, AoXlnR, mediates cellulose-inductive expression of the xylanolytic and cellulolytic genes in Aspergillus oryzae.
AoXlnR was isolated as a transcriptional activator of the major xylanase gene, xynF1, in Aspergillus oryzae. To investigate the spectrum of genes under the control of AoXlnR, expression of the xylanolytic and cellulolytic genes in an A. oryzae wild type strain, an AoxlnR disruptant and an AoXlnR overexpressed strain was analyzed by Northern blotting. AoXlnR mediated expression of at least four xylanolytic genes and four cellulolytic genes when induced by xylan and D-xylose. Moreover, AoXlnR was newly found to mediate the cellulose-inductive expression of the xylanolytic genes as well as the cellulolytic genes.
Marui
Junichiro
J
Department of Biological Mechanisms and functions, Graduate school of Bioagricultural Sciences, Nagoya University, Japan.
Kitamoto
Noriyuki
N
Kato
Masashi
M
Kobayashi
Tetsuo
T
Tsukagoshi
Norihiro
N
D001236:Aspergillus oryzae / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002480:Cellulase / Q000235:genetics*; D002482:Cellulose / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins; D015870:Gene Expression; D005800:Genes, Fungal; D007763:Lac Operon; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000378:metabolism*; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000235:genetics*
D005656:Fungal Proteins; D015534:Trans-Activators; C115443:XlnR protein, Aspergillus; D002482:Cellulose; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase