Changes of serum MDA and SOD levels in ischemia-reperfusion injuries of the limbs in rats preconditioned with heat stress.
OBJECTIVE
To study the effect of pretreatment with heat stress on ischemia-reperfusion injuries of the limbs in rats.


METHODS
Rat models of ischemia-reperfusion injury were established with or without prior heat stress treatment, in which serum malondialdehyde (MDA) level and superoxide dismutase (SOD) activities were measured by means of thiobarbituric acid and nitroblue tetrazolium respectively.


RESULTS
Comparison between ischemia-reperfusion group and the control group revealed significantly elevated MDA levels (P<0.01) and depressed SOD activities (P<0.01) in the condition of ischemia-reperfusion injury. Pretreatment with heat stress, however, significantly lowered the elevated MDA levels (P<0.01) and enhanced SOD activities (P<0.01) during ischemia-reperfusion injury.


CONCLUSIONS
Pretreatment with heat stress can enhance the activity of SOD to remove free oxygen radicals in the cells.
Sun
Yong-Jian
YJ
Department of Orthopedics and Traumatology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Wang
Qian
Q
Pei
Guo-Xian
GX
Zheng
Lei
L
Tan
Xiong-Jin
XJ
D000818:Animals; D005121:Extremities / Q000098:blood supply*; D006358:Hot Temperature; D019194:Ischemic Preconditioning; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood*; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000097:blood*
D008315:Malondialdehyde; D013482:Superoxide Dismutase