title: Amperometric enzyme electrode for organic peroxides determination prepared from horseradish peroxidase immobilized in poly(vinylferrocenium) film.
abstract: Organic peroxides, t-butyl hydroperoxide, 2-butanone peroxide, cumene hydroperoxide and t-butyl peracetate, were determined by an amperometric enzyme electrode. The enzyme electrode was prepared through electrostatic immobilization of horseradish peroxidase (HRP) in a polyvinylferrocenium (PVF) film. A PVF(+)ClO(4)(-) film was coated on a Pt foil at +0.70 V by electrooxidation of polyvinylferrocene in methylene chloride with 0.1 M tetrabutylammonium perchlorate (TBAP). The enzyme modified electrode PVF(+)HRP(-) was prepared by anion-exchange in a solution of HRP(-) in 0.05 M phosphate buffer at pH 8.5. FTIR spectroscopy was used to identify PVF, PVF(+)ClO(4)(-), and PVF(+)HRP(-). The immobilized amount of the enzyme in the film was determined by UV spectroscopy. The effects of the polymeric film thickness, bulk enzyme concentration used in the immobilization treatment and the temperature on the performance of enzyme electrode were investigated. The inhibitory effect of oxygen was also examined. Linearities, lower detection limits, active life times and sensitivities of the electrode were determined for each peroxide.
authors.affiliation: Ankara Nuclear Research and Training Center, 06100 Beşevler, Ankara, Turkey.
authors.forename: Mehtap
authors.initials: M
authors.lastname: Gündoğan-Paul
authors.forename: Serdar S
authors.initials: SS
authors.lastname: Celebi
authors.forename: Haluk
authors.initials: H
authors.lastname: Ozyörük
authors.forename: Attila
authors.initials: A
authors.lastname: Yildiz
mesh_terms: D001555:Benzene Derivatives / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D004566:Electrodes; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005296:Ferrous Compounds; D006735:Horseradish Peroxidase / Q000378:metabolism*; D010545:Peroxides / Q000032:analysis*; D011145:Polyvinyls; D013696:Temperature
chemical_list: D001555:Benzene Derivatives; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D010545:Peroxides; D011145:Polyvinyls; C451099:polyvinylferrocenium; D006735:Horseradish Peroxidase; C007164:cumene hydroperoxide