title: Thermal stability of catalases active in dormant saffron (Crocus sativus L.) corms.
abstract: Catalase activity was detected in crude extract prepared from dormant saffron (Crocus sativus L.) corms. The activity was independent of pH in the range 6.0-11.0. Thermostability studies suggested the presence of three isoenzymes with transition temperatures of 30 degrees C, 45 degrees C and 60 degrees C, respectively, as given by Arrhenius plots. When stained for catalase activity gel electropherograms of extract revealed 3 distinct bands with apparent molecular weight of 323,000, 295,000 and 268,000, respectively. Thus it appeared that at least three isoenzymes of catalase were present in dormant saffron corms.
authors.affiliation: Laboratory for Life Sciences, Saadat Abade, Tehran, Iran. keyhanie@ibb.ut.ac.ir
authors.forename: J
authors.initials: J
authors.lastname: Keyhani
authors.forename: E
authors.initials: E
authors.lastname: Keyhani
authors.forename: J
authors.initials: J
authors.lastname: Kamali
mesh_terms: D002374:Catalase / Q000737:chemistry / Q000378:metabolism*; D027622:Crocus / Q000201:enzymology*; D004795:Enzyme Stability; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D016877:Oxidants / Q000378:metabolism; D018514:Plant Structures / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D016877:Oxidants; D006861:Hydrogen Peroxide; D002374:Catalase