Effect of cholesterol on the interaction of seminal plasma protein, PDC-109 with phosphatidylcholine membranes.
Binding of PDC-109, the major protein of the bovine seminal plasma, to sperm plasma membrane results in an efflux of cholesterol and choline phospholipids, a necessary event before capacitation can occur. The selectivity of PDC-109 for different spin-labelled phospholipids and sterol probes in dimyristoylphosphatidylcholine (DMPC) host matrix has been characterized earlier by EPR spectroscopy [Ramakrishnan, M., Anbazhagan, V., Pratap, T.V., Marsh, D. and Swamy, M.J. (2001) Biophys. J. 81, 2215-2225]. In this report the effect of cholesterol on the interaction of PDC-109 with DMPC membranes has been investigated by spin-label EPR spectroscopy. The results indicate that the presence of cholesterol leads to an increased association of different phospholipid as well as sterol probes, thus modulating the interaction of PDC-109 with phospholipid membranes.
Swamy
Musti J
MJ
School of Chemistry, University of Hyderabad, India. mjssc@uohyd.ernet.in
Marsh
D
D
Anbazhagan
V
V
Ramakrishnan
M
M
D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000378:metabolism / Q000494:pharmacology*; D004134:Dimyristoylphosphatidylcholine / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D066298:In Vitro Techniques; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D008954:Models, Biological; D029607:Seminal Vesicle Secretory Proteins / Q000187:drug effects* / Q000378:metabolism*; D013113:Spin Labels
D008563:Membrane Lipids; D029607:Seminal Vesicle Secretory Proteins; D013113:Spin Labels; C424303:seminal vesicle secretory protein 109, Bos taurus; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine