title: Minimum inhibitory concentration breakpoints and disk diffusion inhibitory zone interpretive criteria for tilmicosin susceptibility testing against Pasteurella multocida and Actinobacillus pleuropneumoniae associated with porcine respiratory disease.
abstract: Tilmicosin is a novel macrolide antibiotic developed for exclusive use in veterinary medicine. Tilmicosin has been approved as a feed premix to control porcine respiratory disease associated with Pasteurella multocida and Actinobacillus pleuropneumoniae. The development of antimicrobial susceptibility testing guidelines for tilmicosin was predicated on the relationship of clinical efficacy studies that demonstrated a favorable therapeutic outcome, on pharmacokinetic data, and on in vitro test data, as recommended by the National Committee for Clinical Laboratory Standards (NCCLS). The approved breakpoints for the minimum inhibitory concentration dilution testing for both species are resistant, > or = 32 microg/ml, and susceptible, < or = 16 microg/ml. The zone of inhibition interpretive criteria for disk diffusion testing with a 15-microg tilmicosin disk are resistant, < or = 10 mm, and susceptible, > or = 11 mm.
authors.affiliation: Elanco Animal Health, Greenfield, IN 46140, USA.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Shryock
authors.forename: J Mitchell
authors.initials: JM
authors.lastname: Staples
authors.forename: David C
authors.initials: DC
authors.lastname: DeRosa
mesh_terms: D000189:Actinobacillus Infections / Q000188:drug therapy / Q000382:microbiology* / Q000662:veterinary; D016977:Actinobacillus pleuropneumoniae / Q000187:drug effects* / Q000302:isolation & purification; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004058:Diffusion; D024881:Drug Resistance, Bacterial; D004917:Erythromycin / Q000494:pharmacology / Q000627:therapeutic use; D018942:Macrolides; D008826:Microbial Sensitivity Tests; D010326:Pasteurella Infections / Q000188:drug therapy / Q000382:microbiology* / Q000662:veterinary; D016979:Pasteurella multocida / Q000187:drug effects* / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy / Q000382:microbiology*; D015645:Tylosin / Q000031:analogs & derivatives* / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; D004917:Erythromycin; C052319:tilmicosin; D015645:Tylosin