title: Molecular analysis of microscopic ezrin dynamics by two-photon FRAP.
abstract: Ezrin plays a key role in coupling signal transduction to cortical cell organization. This actin-membrane linker undergoes a series of conformational changes that modulate its interactions with various partners and its localization in membrane or cytosolic pools. Its mobility and exchange rates within and between these two pools were assessed by two-photon fluorescence recovery after photobleaching in epithelial cell microvilli. Analysis of ezrin mutants with an altered actin-binding site revealed three ezrin membrane states of different mobilities and exchange properties, reflecting sequential association with membrane components and F-actin in the context of a fast overall turnover.
authors.affiliation: Laboratoires de Physico-Chimie,Unité Mixte de Recherche 168 and Morphogenèse et Signalisation Cellulaires, Unité Mixte de Recherche 144, Centre National de la Recherche Scientifique/Institut Curie, 26, Rue d'Ulm, 75248 Paris Cedex 05, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Coscoy
authors.forename: François
authors.initials: F
authors.lastname: Waharte
authors.forename: Alexis
authors.initials: A
authors.lastname: Gautreau
authors.forename: Marianne
authors.initials: M
authors.lastname: Martin
authors.forename: Daniel
authors.initials: D
authors.lastname: Louvard
authors.forename: Paul
authors.initials: P
authors.lastname: Mangeat
authors.forename: Monique
authors.initials: M
authors.lastname: Arpin
authors.forename: Françis
authors.initials: F
authors.lastname: Amblard
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary / Q000378:metabolism; D049452:Green Fluorescent Proteins; D008027:Light; D008164:Luminescent Proteins / Q000378:metabolism; D008954:Models, Biological; D010750:Phosphoproteins / Q000737:chemistry* / Q000502:physiology*; D017785:Photons; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013552:Swine; D014162:Transfection; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D000099149:Ezrin