cDNA cloning and expression analysis of a novel human F-box only protein.
F-box proteins are an expanding family of eukaryotic proteins that are characterized by an approximately 40 amino acid motif. Some F-box proteins are critical for the controlled degradation of cellular regulatory proteins. During a large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a cDNA clone that encodes a novel F-box protein. It showed a 90.0% identity with the previously isolated mouse F-box protein16 at the amino acid level. Northern blot analysis showed no detectable expression, while reverse transcription-polymerase chain reaction analysis indicated that FBXO16 was expressed in the heart, spleen, and colon. By mapping, we localized the FBXO16 gene to the human chromosome 8p12. The FBXO16 gene consisted of 9 exons that spanned 67,816 bp of human genomic DNA.
Cheng
Haipeng
H
State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, China.
Ma
Yushu
Y
Ni
Xiaohua
X
Jiang
Min
M
Guo
Lingchen
L
Jin
Wei
W
Xu
Weiwen
W
Cao
Gentao
G
Ji
Chaoneng
C
Yin
Kang
K
Gu
Shaohua
S
Ma
Yuhong
Y
Xie
Yi
Y
Mao
Yumin
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D018076:DNA, Complementary; D044783:F-Box Proteins; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D044783:F-Box Proteins; C473095:FBXO16 protein, human; D011506:Proteins