title: Rotenone-insensitive NADH dehydrogenase is a potential source of superoxide in procyclic Trypanosoma brucei mitochondria.
abstract: The rotenone-insensitive NADH dehydrogenase isolated from mitochondria of the procyclic form of Trypanosoma brucei has the ability to produce superoxide anions (Biochemistry 41 (2002) 3065). Superoxide production by the purified enzyme was 60% inhibited by diphenyl iodonium (DPI), stimulated significantly by ubiquinone analogues, and unaffected by metal ions. Production of reactive oxygen species (ROS) in intact cells was not affected by addition of rotenone with proline and malate as substrates; however, addition of rotenone inhibited 41% ROS production with succinate as substrate. These results suggest that complex I is not involved in production of ROS and that succinate-linked reversed electron transport occurs in trypanosome mitochondria. Superoxide formation in mitochondria with NADH as substrate was stimulated by antimycin A but was unaffected by myxothiazol plus stigmatellin, indicating that bc(1) complex is not a source of superoxide. DPI and fumarate inhibited by 68 and 36%, respectively, the rate of superoxide production with NADH as substrate. Addition of both fumarate and DPI blocked 70% superoxide production in mitochondria, a total inhibition similar to that observed with DPI addition alone. These results suggest that the rotenone-insensitive NADH dehydrogenase in addition to NADH fumarate reductase is a potential source of superoxide production in procyclic trypanosome mitochondria.
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, P.O. Box 9142, West Virginia University School of Medicine, Morgantown, WV 26506-9142, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.forename: Diana S
authors.initials: DS
authors.lastname: Beattie
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000494:pharmacology; D005650:Fumarates / Q000494:pharmacology; D008293:Malates / Q000378:metabolism; D008689:Methacrylates; D008928:Mitochondria / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism*; D009861:Onium Compounds / Q000494:pharmacology; D011090:Polyenes / Q000494:pharmacology; D011392:Proline / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology*; D013379:Substrate Specificity; D019802:Succinic Acid / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013844:Thiazoles / Q000494:pharmacology; D014346:Trypanosoma brucei brucei / Q000201:enzymology*; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000494:pharmacology; D014475:Uncoupling Agents / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001713:Biphenyl Compounds; D005650:Fumarates; D008293:Malates; D008689:Methacrylates; D009861:Onium Compounds; D011090:Polyenes; D013844:Thiazoles; D014475:Uncoupling Agents; D012402:Rotenone; D009243:NAD; C031291:diphenyliodonium; D013481:Superoxides; D014451:Ubiquinone; D000968:Antimycin A; C030517:myxothiazol; C030298:malic acid; C032005:fumaric acid; C041573:stigmatellin; D011392:Proline; D019802:Succinic Acid; D009245:NADH Dehydrogenase