title: Thyroid gland function in ovariectomized ewes exposed to phytoestrogens.
abstract: Phytoestrogens are by definition plant-derived substances that are able to activate the mammalian oestrogen receptors. We examined the possible effects of phytoestrogens on the secretion of thyroid hormones as well as on the immunoreactivity to oestrogen receptor alpha (ER alpha) in the thyroid glands of ovariectomized ewes. Eight ovariectomized ewes were fed 3.5 kg of 100% red clover silage for 14 days. Blood samples were collected before and on day 14 of exposure to phytoestrogens. After 5 months, four of the ewes were re-exposed to red clover silage as described above and the other four served as controls. Blood samples were collected as above. All ewes were slaughtered at the end of the experiment and the thyroid glands were weighed and examined for macroscopical changes. Tissue samples were taken for immunohistochemistry and image analysis. Ewes exposed to red clover silage had significantly higher plasma concentrations of total T(3) and free T(3) than ewes fed hay. The cross-section area of thyroid follicles tended to be larger in ewes fed red clover silage than in the control animals. ER alpha immunoreactivity was stronger in thyroid glands from ewes exposed to phytoestrogens than in ewes fed hay. In conclusion, daily ingestion of 81-95 mg phytoestrogens per kg body weight for 14 days stimulated secretion of thyroid hormones and tended to increase follicle size and ER alpha immunoreactivity of thyroid glands of ovariectomized ewes.
authors.affiliation: Department of Animal Physiology, Centre for Reproductive Biology in Uppsala, Swedish University of Agricultural Sciences (SLU), PO Box 7045, S-750 07, Uppsala, Sweden. andrzej.madej@djfys.slu.se
authors.forename: Andrzej
authors.initials: A
authors.lastname: Madej
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Persson
authors.forename: Torbjörn
authors.initials: T
authors.lastname: Lundh
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Ridderstråle
mesh_terms: D000818:Animals; D047628:Estrogen Receptor alpha; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D005260:Female; D007150:Immunohistochemistry; D007529:Isoflavones; D010052:Ovariectomy; D048789:Phytoestrogens; D028321:Plant Preparations; D011960:Receptors, Estrogen / Q000378:metabolism; D013961:Thyroid Gland / Q000378:metabolism / Q000502:physiology*
chemical_list: D047628:Estrogen Receptor alpha; D004968:Estrogens, Non-Steroidal; D007529:Isoflavones; D048789:Phytoestrogens; D028321:Plant Preparations; D011960:Receptors, Estrogen