A special photoproduct of UV-irradiated DNA in vacuo.
DNA isolated from E. coli B/r cells was UV-irradiated at 254 nm under high vacuum of 10(-7) torr. Compared with DNA in solution, the formation of cis-syn thymine dimers (TT) was decreased, and the formation of cytosine thymine dimers (CT) and a photoproduct of RF value 0.41 was increased. The latter photoproduct was identified as tran-syn TT which is preferentially produced in heat-denaturated DNA.
Bucker
H
H
DFVLR, Institut fur Flugmedizin, Frankfurt/M. FRG.
Dose
K
K
Horneck
G
G
Thomas
C
C
D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects*; D019281:Dimerization; D004926:Escherichia coli; D005118:Extraterrestrial Environment; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000138:chemical synthesis* / Q000378:metabolism; D011853:Radiobiology; D013171:Spores, Bacterial / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays; D014618:Vacuum
D004269:DNA, Bacterial; D011740:Pyrimidine Dimers