Constitutive activation of the LH receptor is associated with an alteration in the conformation of the ectodomain.
The human LH receptor (hLHR) consists of a heptahelical segment prototypical of G protein-coupled receptors and a large ectodomain. The binding of hormone to the ectodomain or the presence of activating mutations stabilize the hLHR in an active conformation. Although it has been inferred that activation of the hLHR involves conformational alterations, direct evidence supporting this has not been reported. We have addressed this issue by comparing the protease sensitivity of the wild-type hLHR as compared to three activating mutants of the hLHR. Our data demonstrate that the ectodomains of the activating hLHR mutants are more susceptible to proteolysis as compared to the wild-type hLHR. As such, they provide the first experimental data demonstrating that the conformations of the active and inactive states of the hLHR are distinct and suggest that activation of the hLHR (at least as induced by mutations) involves a tighter interaction of the ectodomain with the heptahelical segment of the receptor as opposed to a release of the ectodomain from the heptahelical segment.
Jaquette
Julie
J
Department of Physiology and Biophysics, The University of Iowa College of Medicine, Iowa City, IA 52242, USA.
Segaloff
Deborah L
DL
D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D009154:Mutation; D010449:Peptide Mapping; D011485:Protein Binding / Q000235:genetics; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011974:Receptors, LH / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D011974:Receptors, LH; D010450:Endopeptidases