title: Functional analysis of yeast gene families involved in metabolism of vitamins B1 and B6.
abstract: In order to clarify their physiological functions, we have undertaken a characterization of the three-membered gene families SNZ1-3 and SNO1-3. In media lacking vitamin B(6), SNZ1 and SNO1 were both required for growth in certain conditions, but neither SNZ2, SNZ3, SNO2 nor SNO3 were required. Copies 2 and 3 of the gene products have, in spite of their extremely close sequence similarity, slightly different functions in the cell. We have also found that copies 2 and 3 are activated by the lack of thiamine and that the Snz proteins physically interact with the thiamine biosynthesis Thi5 protein family. Whereas copy 1 is required for conditions in which B(6) is essential for growth, copies 2 and 3 seem more related with B(1) biosynthesis during the exponential phase.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universitat de València, C/Dr Moliner 50, E-46100, Burjassot, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Rodríguez-Navarro
authors.forename: Bertrand
authors.initials: B
authors.lastname: Llorente
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Rodríguez-Manzaneque
authors.forename: Anna
authors.initials: A
authors.lastname: Ramne
authors.forename: Genoveva
authors.initials: G
authors.lastname: Uber
authors.forename: Denis
authors.initials: D
authors.lastname: Marchesan
authors.forename: Bernard
authors.initials: B
authors.lastname: Dujon
authors.forename: Enrique
authors.initials: E
authors.lastname: Herrero
authors.forename: Per
authors.initials: P
authors.lastname: Sunnerhagen
authors.forename: José E
authors.initials: JE
authors.lastname: Pérez-Ortín
mesh_terms: D015152:Blotting, Northern; D003470:Culture Media; D015966:Gene Expression Regulation, Fungal; D005810:Multigene Family / Q000502:physiology*; D011730:Pyridoxal; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D013831:Thiamine / Q000096:biosynthesis / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D025101:Vitamin B 6 / Q000096:biosynthesis / Q000378:metabolism*; D024483:Vitamin K 3; D019759:Carbon-Nitrogen Lyases
chemical_list: D003470:Culture Media; D011730:Pyridoxal; D012331:RNA, Fungal; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D013831:Thiamine; D025101:Vitamin B 6; D024483:Vitamin K 3; C103168:SNZ1 protein, S cerevisiae; D019759:Carbon-Nitrogen Lyases