title: Establishing lymphoblastoid cell lines from frozen blood of extremely old individuals.
abstract: In population studies involving peripheral blood samples from nonagenarian and centenarian donors, the amount of biological material available for research is restricted, as only a few millilitres of blood can be obtained from extremely old donors without significantly compromising their health. Here we describe a protocol to immortalize small amounts of total frozen blood from extremely old donors (90+) using the Epstein-Barr virus, despite the low level of circulating B cells present in nonagenarian and centenarian blood samples. This methodology provides a unique way to maximize resources of biological material available for research.
authors.affiliation: Department of Anatomy and Cell Biology, McGill University, 3640 University Street, Que, Montreal, Canada.
authors.forename: Chantale
authors.initials: C
authors.lastname: Lacelle
authors.forename: Eugenia
authors.initials: E
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008875:Middle Aged; D010949:Plasma
keywords: Non-programmatic