title: Changes in oligomerization are essential for the chaperone activity of a small heat shock protein in vivo and in vitro.
abstract: The ability of small heat shock proteins (sHSPs) to prevent thermal aggregation of other proteins may require disassembly and reassembly of sHSP oligomers. We investigated the role of changes in sHSP oligomerization by studying a mutant with reduced oligomeric stability. In HSP16.6, the single sHSP in the cyanobacterium Synechocystis sp. PCC 6803, the mutation L66A causes oligomer instability and reduced chaperone activity in vitro. Because thermotolerance of Synechocystis depends on HSP16.6, a phenotype that is enhanced in a deltaClpB1 strain, the effect of mutations can also be assayed in vivo. L66A causes severe defects in thermotolerance, suggesting that oligomeric stability of sHSPs is required for cellular function. This hypothesis was supported by a selection for intragenic suppressors of L66A, which identified mutations that stabilize oligomers of both L66A and wild-type HSP16.6. Analysis of both over- and under-oligomerizing mutants suggests that sHSPs must disassemble before they can release substrates. Furthermore, the suppressor mutations not only restore in vivo activity to L66A, they also ameliorate chaperone defects in vitro, and thus provide the first direct evidence for a chaperone function of an sHSP in cellular thermotolerance.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA.
authors.forename: Kim C
authors.initials: KC
authors.lastname: Giese
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Vierling
mesh_terms: D001704:Biopolymers; D000458:Cyanobacteria / Q000737:chemistry / Q000235:genetics / Q000254:growth & development; D016153:Genes, Suppressor; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D010957:Plasmids
chemical_list: D001704:Biopolymers; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones