Hydrophilic interaction liquid chromatography/electrospray mass spectrometry determination of acyclovir in pregnant rat plasma and tissues.
Reversed-phase chromatography is the most common means of separation for small drug molecules. However, polar drugs may suffer from poor retention and peak shape in reversed-phase high-performance liquid chromatography (RP-HPLC). Hydrophilic interaction liquid chromatography (HILIC) provides a viable alternative to RP-HPLC and is an excellent way to separate polar compounds. This paper describes a HILIC/ESI-MS/MS assay for the determination of acyclovir from rat plasma, amniotic fluid, placental tissue, and fetal tissue. The isocratic separation utilizes an underivatized silica column with an acetonitrile/formate buffer mobile phase (80:20). The method is validated over a range of 50 ng/mL to 50 micro g/mL with % error and % relative standard deviation of <15% over 3 days. All samples are prepared by acetonitrile protein precipitation, which yields high recovery (>84% for acyclovir). This assay can be applied to the pharmacokinetic study of the placental transfer of acyclovir.
Brown
Stacy D
SD
College of Pharmacy, Department of Pharmaceutical and Biomedical Sciences, The University of Georgia, Athens, GA 30602-2353, USA.
White
Catherine A
CA
Bartlett
Michael G
MG
D000212:Acyclovir / Q000032:analysis* / Q000097:blood; D000653:Amniotic Fluid / Q000737:chemistry; D000818:Animals; D000998:Antiviral Agents / Q000032:analysis* / Q000097:blood; D002021:Buffers; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005333:Fetus / Q000737:chemistry / Q000378:metabolism; D007202:Indicators and Reagents; D008431:Maternal-Fetal Exchange; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
D000998:Antiviral Agents; D002021:Buffers; D007202:Indicators and Reagents; D000212:Acyclovir