[Similarities in periodical structures in the position of nucleotides in regions of initiation of replication of bacterial genomes].
The regions of initiation of replication of some bacterial genomes were studied by the method of Fourier matrix analysis. A generalized spectral portrait of the primary structures of E. coli-like regions of initiation of replication in bacteria was obtained, which reflects the features of their structural and functional organization. It contains well-pronounced peaks that correspond to the periods T = 2, 11, 17, 27, 86-105 of nucleotides. The peaks corresponding to T = 9, 13, 14, 18, 19, 33-35, 45-47, 74-85, 106-110 are less pronounced. The uniqueness of the Fourier spectrum corresponding to the region of initiation of replication of E. coli oriC was considered by the example of the complete genome of E. coli. Some regions of the E. coli genome were identified that differ from oriC in the primary structure but have Fourier spectra resembling the spectrum of oriC. A number of these regions are alternative points of initiation of replication in sdrA(rnh) mutants of E. coli, the others are localized in yet unidentified regions of the E. coli genome but are capable, in our opinion, to participate in the initiation of replication. Thus, from the similarity of spectral portraits of different regions of the genome, it was possible to reveal several regions that have similar functions, i.e., are involved in initiation of replication.
Kravatskaia
G I
GI
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 117984 Russia.
Frank
G K
GK
Makeev
V Iu
VIu
Esipova
N G
NG
D001483:Base Sequence; D004755:Enterobacteriaceae / Q000235:genetics; D005583:Fourier Analysis; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000235:genetics*; D018741:Replication Origin; D014733:Vibrio / Q000235:genetics
D009711:Nucleotides