title: Genetic variability in Hoplias malabaricus (Osteichthyes: Erythrinidae) in fluvial and lacustrine environments in the Upper Paraná river floodplain (Paraná State, Brazil).
abstract: Genetic variability in Hoplias malabaricus, from two localities in the upper Paraná Riverfloodplain, was investigated by starch and polyacrylamide gel electrophoresis. A total of 52 specimens were analyzed for 14 enzymatic systems. Twenty-three gene loci of 13 enzymatic systems (AAT, ACP, ADH, GDH, G6PDH, GPI, IDH, LDH, MDH, MEP, PGM, PER, and SOD) were analyzed by starch gel electrophoresis (Penetrose-30). The EST system was analyzed by polyacrylamide gel electrophoresis, and one polymorphic locus was found (EST-1). Twenty-four loci were detected. The proportion of polymorphic loci was 37.5% in the lagoon and 33.3% in the river. Significant differences in allele frequencies of five loci were found between specimens from the two environments. Expected mean heterozygosity (He = 0.14) is the same in the river and lagoon, however, Nei's genetic distance (D) between the population of the two locations was 0.049.
authors.affiliation: Nupelia, Núcleo de Pesquisas em Limnologia, Ictiologia e Aqüicultura).
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Peres
authors.forename: Erasmo
authors.initials: E
authors.lastname: Renesto
authors.forename: Ana Sílvia
authors.initials: AS
authors.lastname: Lapenta
authors.forename: Cláudio Henrique
authors.initials: CH
authors.lastname: Zawadzki
mesh_terms: D000818:Animals; D001938:Brazil; D017753:Ecosystem; D004592:Electrophoresis, Starch Gel; D004798:Enzymes / Q000235:genetics*; D005399:Fishes / Q000235:genetics* / Q000254:growth & development; D005618:Fresh Water; D014644:Genetic Variation; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism
chemical_list: D004798:Enzymes; D007527:Isoenzymes