title: Identification of a role for actin in translational fidelity in yeast.
abstract: Numerous studies have suggested a role for actin in translation, but the molecular details of this role are unknown. To elucidate the function(s) of actin in translation, we have studied 25 isogenic, conditional yeast actin mutants. Strikingly, analysis of these mutants indicates that none of those tested have conditional growth defects caused by reduced rates of protein synthesis; and analysis of latrunculin A-treated wild-type cells indicates that even complete disruption of the actin cytoskeleton has no significant effect on the rate of translation. However, analysis of the effect of the 25 actin mutations on fidelity and sensitivity to translation inhibitors identified two mutations ( act1-2 and act1-122) that cause a significant reduction in the fidelity of translation, as assayed by nonsense suppression, and several mutants that are sensitive to paromomycin, which affects translational fidelity. Translation elongation factor 1A (eEF1A) also has a role in fidelity, and in the presence of excess eEF1A four of the mutants ( act1-2, act1-20, act1-120, and act1-125) are even more sensitive to paromomycin, while one mutant ( act1-122) becomes less sensitive. Together, these findings suggest that actin may not be important for the rate of translation, but may have a critical role in ensuring translational fidelity.
authors.affiliation: Department of Biology, St. Olaf College, Northfield, MN 55057, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Kandl
authors.forename: R
authors.initials: R
authors.lastname: Munshi
authors.forename: P A
authors.initials: PA
authors.lastname: Ortiz
authors.forename: G R
authors.initials: GR
authors.lastname: Andersen
authors.forename: T G
authors.initials: TG
authors.lastname: Kinzy
authors.forename: A E M
authors.initials: AE
authors.lastname: Adams
mesh_terms: D000199:Actins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000483:Alleles; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002455:Cell Division; D003599:Cytoskeleton / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D016366:Open Reading Frames / Q000235:genetics; D020648:Peptide Elongation Factor 1 / Q000378:metabolism; D010641:Phenotype; D014176:Protein Biosynthesis / Q000187:drug effects; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics; D013489:Suppression, Genetic; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines
chemical_list: D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D020648:Peptide Elongation Factor 1; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D013844:Thiazoles; D053778:Thiazolidines; C037067:latrunculin A