[Determination of lignan glycosides in the roots of Gaultheria yunnanensis Rehd. by HPLC].
OBJECTIVE
To determine the contents of four lignan glycosides in the root of Gaultheria yunnanensis, namely, D1: gaultheroside A, D2:(-)-8'-methoxyisolariciresinol-2a-O-beta-D-xylopyranoside, D3:(-)-isolariciresinol-2a-O-beta-D-xylopyranside, D4:(+)-lyoniresinol-2a-O-beta-D-glucoxylopyranoside.


METHODS
Rp-HPLC was used to separate and analyze the lignan glycosides in the root of the title plant.


RESULTS
The method for sample preparation was set up; the gradient program for the mobile phase was established; and the retention times of four lignan glycosides were determined.


CONCLUSIONS
The method has been proved to be accurate, reproducible and of higher recovery rate.
Zhang
Z
Z
School of Pharmaceutical Sciences, Beijing Medical University, Beijing 100083.
Guo
D
D
Li
C
C
Zheng
J
J
Koike
K
K
Jia
Z
Z
Nikaido
T
T
Fan
G
G
Duan
Y
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D031282:Gaultheria / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis*; D017705:Lignans / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D006027:Glycosides; D017705:Lignans; C471257:gaultheroside A