New Rev-transport inhibitor with anti-HIV activity from Valerianae Radix.
Bioassay-guided separation by use of the fission yeast expressing NES of Rev, a HIV-1 viral regulatory protein, resulted in isolation of valtrate (1) as a new Rev-transport inhibitor from the nucleus to cytoplasm from Valerianae Radix. Valtrate (1) also inhibited the p-24 production of HIV-1 virus without showing any cytotoxicity against the host MT-4 cells.
Murakami
Nobutoshi
N
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka, Japan.
Ye
Ying
Y
Kawanishi
Motoyuki
M
Aoki
Shunji
S
Kudo
Nobuaki
N
Yoshida
Minoru
M
Nakayama
Emi E
EE
Shioda
Tatsuo
T
Kobayashi
Motomasa
M
D001710:Biotin; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification / Q000494:pharmacology; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D054303:HIV Reverse Transcriptase; D006801:Humans; D039823:Iridoids / Q000302:isolation & purification* / Q000494:pharmacology*; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D014632:Valerian / Q000737:chemistry*
D005231:Fatty Acids, Unsaturated; D016655:HIV Core Protein p24; D039823:Iridoids; D018894:Reverse Transcriptase Inhibitors; C421091:callystatin A; D001710:Biotin; D054303:HIV Reverse Transcriptase; C030127:valtrate; C038753:leptomycin B