Apisimin, a new serine-valine-rich peptide from honeybee (Apis mellifera L.) royal jelly: purification and molecular characterization.
A peptide named apisimin was found in honeybee (Apis mellifera L.) royal jelly (RJ). N-terminal sequencing showed that this peptide corresponded to the sequence of a cDNA clone isolated from an expression cDNA library prepared from heads of nurse honeybees. No homology was found between the protein sequence of apisimin with a molecular mass of 5540.4 Da and sequences deposited in the Swiss-Prot database. The 54 amino acids of apisimin do not include Cys, Met, Pro, Arg, His, Tyr, and Trp residues. The peptide shows a well-defined secondary structure as observed by CD spectroscopy, and has the tendency to form oligomers. Isoelectrofocusing showed apisimin to be an acidic peptide.
Bíliková
K
K
Laboratory of Genetic Engineering, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84238 Bratislava, Slovak Republic.
Hanes
J
J
Nordhoff
E
E
Saenger
W
W
Klaudiny
J
J
Simúth
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000737:chemistry* / Q000235:genetics; D002942:Circular Dichroism; D018076:DNA, Complementary / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry*; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017433:Protein Structure, Secondary; D012694:Serine / Q000032:analysis; D014633:Valine / Q000032:analysis; D000099219:Royal Jelly
D018076:DNA, Complementary; D005227:Fatty Acids; D019476:Insect Proteins; D012694:Serine; D014633:Valine; D000099219:Royal Jelly