Gene delivery to the rat liver using cationic lipid emulsion/DNA complex: comparison between intra-arterial, intraportal and intravenous administration.
OBJECTIVE
To compare the efficiency of intra-arterial, intraportal, and intravenous administration of cationic lipid emulsion/DNA complex, as used for gene transfer to rat liver.


METHODS
DNA-carrier complex for the in-vivo experiment was prepared by mixing DNA and a cationic lipid emulsion. According to the administration route used (intra-arterial, intraportal, or intravenous), the animals were assigned to one of three groups. The heart, lung, liver, spleen and kidneys were removed and assayed for total protein and luciferase concentration.


RESULTS
The cationic lipid emulsion/DNA complex used successfully transfected the various organs via the different administration routes employed. Luciferase activity in each organ of untreated animals was negligible. Liver luciferase values were significantly higher in the groups in which intra-arterial or intraportal administration was used.


CONCLUSIONS
The intra-arterial or intraportal administration of cationic lipid emulsion/DNA complex is superior to intravenous administration and allows selective gene transfer to the liver.
Choi
Bo Yoon
BY
Department of Radiology, Seoul National University College of Medicine, Seoul, Korea.
Chung
Jin Wook
JW
Park
Jae Hyung
JH
Kim
Keon Ha
KH
Kim
Young Il
YI
Koh
Young Hwan
YH
Kwon
Jong Won
JW
Lee
Kyoung Ho
KH
Choi
Hyuk Jae
HJ
Kim
Tae Woo
TW
Kim
Young Jin
YJ
Chung
Hesson
H
Kwon
Ik Chan
IC
Jeong
Seo Young
SY
D000818:Animals; D002404:Catheterization; D002412:Cations / Q000008:administration & dosage*; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004655:Emulsions / Q000008:administration & dosage; D018014:Gene Transfer Techniques; D007269:Injections, Intra-Arterial; D007275:Injections, Intravenous; D008055:Lipids / Q000008:administration & dosage* / Q000235:genetics; D008099:Liver / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002412:Cations; D004655:Emulsions; D008055:Lipids; D004247:DNA