title: [Study of the functional role of Ctp-family proteins in Synechocystis sp. PCC 6803 cyanobacteria].
abstract: To understand the functional role of CtpB and CtpC proteins, which are similar to the C-terminal processing CtpA peptidase, the effect of the insertional inactivation of the ctpB and ctpC genes on the phenotypic characteristics of Synechocystis sp. PCC 6803 was studied. The inactivation of the ctpC gene was found to be lethal to the cyanobacterium, which indicates a vital role of the CtpC protein. The mutant with the inactivated ctpB gene had the same photosynthetic characteristics as the wild-type strain. The double mutant@[delta]ctpA delta ctpB with the two deleted genes was identical, in the phenotypic characteristics, to the mutant with a knock-out mutation in the ctpA gene, which was unable to grow photoautotrophically. The data obtained suggest that, in spite of the high similarity of the Ctp proteins, they serve different functions in Synechocystis sp. PCC 6803 cells and cannot compensate for each other.
authors.affiliation: Department of Genetics and Selection, Faculty of Biology, Moscow State University, Vorob'evy gory, Moscow, 119899 Russia.
authors.forename: N B
authors.initials: NB
authors.lastname: Ivleva
authors.forename: K V
authors.initials: KV
authors.lastname: Sidoruk
authors.forename: Kh B
authors.initials: KhB
authors.lastname: Pakrasi
authors.forename: S V
authors.initials: SV
authors.lastname: Shestakov
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers