[Analysis with DNA chips of the changes of gene expressions in K562 cells in response to As2O3 treatment].
OBJECTIVE
To investigate differential gene expression in apoptotic cells induced by As(2)O(3), and identify novel apoptosis-related genes.


METHODS
Apoptosis of K562 cells cultured in RPMI 1640 medium supplemented with 10 % calf serum was induced by As(2)O(3). Total RNA of the apoptotic and normal cells were then extracted, purified and subject to reverse transcription into first-strand cDNA, labeled with Cy3/Cy5. Placenta DNA microarrays containing 348 DNA fragments were used to analyze the changes in gene expressions in the cells treated with As(2)O(3).


RESULTS
Eleven differentially expressed genes were identified in the apoptotic cells in comparison with the normal cells, 3 of which were associated with apoptosis, while the others were related to cell growth and proliferation.


CONCLUSIONS
The placenta DNA microarrays we constructed may well apply to the analysis of the differentially expressed genes.
Feng
Chun-qiong
CQ
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China. feng1215@fimmu.com
Ma
Wen-li
WL
Li
Ling
L
Song
Yan-bin
YB
Shi
Rong
R
Wu
Qing-hua
QH
Guo
Qiu-ye
QY
Zhu
Ji
J
Zheng
Wen-ling
WL
D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010087:Oxides / Q000494:pharmacology*
D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D000077237:Arsenic Trioxide