Biomonitoring of urine mutagenicity with the Ames test: improvement of the extraction/concentration method.
Comparative extraction efficiency of the pre-packed Bakerbond-spe-SDB-1 resin and of Amberlite-AD2 (XAD-2) resin, for the preparation of urine extracts in biomonitoring studies. Urine extracts were prepared in parallel with the Bakerbond column and with the classical XAD-2 resin from urines (1) spiked with mutagenic chemicals, (2) collected from patients after chemotherapy, and (3) from smokers. Mutagenic activities were evaluated on Salmonella typhimurium tester strains TA97a, TA98, TA100 and TA102 with and without S9 mix. Mutagenic activities obtained with Bakerbond extracts were almost always higher or at least equivalent to those prepared on XAD-2 resin. Similar results were observed for the three urine sample groups. When fully validated, the use of the pre-packed columns will be more convenient and time-saving for large population studies.
Andre
Véronique
V
Laboratoire de Cancérologie Expérimentale, Centre François Baclesse, GRECAN (Université de Caen-Basse-Normandie), Route de Lion sur Mer, 14076 Cedex 5, Caen, France. v.andre@baclesse.fr
Lebailly
Pierre
P
Deslandes
Edwige
E
Henry-Amar
Michel
M
Gauduchon
Pascal
P
D000970:Antineoplastic Agents / Q000652:urine*; D002852:Chromatography, Ion Exchange; D005260:Female; D006801:Humans; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D011137:Polystyrenes / Q000737:chemistry; D012486:Salmonella typhimurium / Q000187:drug effects*; D012907:Smoking / Q000652:urine*
D000970:Antineoplastic Agents; D009153:Mutagens; D011137:Polystyrenes; C003771:Amberlite XAD-2 resin