Direct evidence for excitonically coupled chlorophylls a and b in LHC II of higher plants by nonlinear polarization spectroscopy in the frequency domain.
Occurrence of excitonic interactions in light-harvesting complex II (LHC II) was investigated by nonlinear polarization spectroscopy in the frequency domain (NLPF) at room temperature. NLPF spectra were obtained upon probing in the chlorophyll (Chl) a/b Soret region and pumping in the Q(y) region. The lowest energy Chl a absorbing at 678 nm is strongly excitonically coupled to Chl b.
D002942:Circular Dichroism; D007834:Lasers; D045342:Light-Harvesting Protein Complexes; D018532:Pisum sativum; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D018515:Plant Leaves; D013053:Spectrophotometry; D013057:Spectrum Analysis / Q000379:methods*; D013696:Temperature
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins