Nitric oxide synthesis and severity of human periodontal disease.
The expression of the inducible nitric oxide synthase enzyme (iNOS) is a response to an inflammatory stimulus and produces a large amount of nitric oxide (NO), which may act as a cytotoxic molecule against the invading microorganism and may be related to both harmful and beneficial effects to tissues.
In order to further characterize the presence of NO in human periodontal disease, we undertook a quantitative study of iNOS positive cells in samples of clinically healthy gingival tissues, plaque-induced gingivitis and localized chronic periodontitis using immunohistochemistry.


RESULTS
A significant increase in the number of iNOS+ cells mm-2 was found in the samples of the gingivitis and periodontitis compared with those of the control. In all groups most of the polymorphonuclear cells showed intense immunoreactivity for iNOS independent of the disease stage, and the percentage of iNOS+ polymorphonuclear cells increased significantly in periodontal disease when compared with the control.


CONCLUSIONS
Our results indicate that iNOS increases in the presence of periodontal disease. In addition, our findings suggest that polymorphonuclear cells present an additional activation pathway in periodontal disease, expressing significant iNOS and probably representing an important source of NO in human periodontal disease that has not been previously reported.
D000704:Analysis of Variance; D002908:Chronic Disease; D003773:Dental Plaque / Q000150:complications; D004848:Epithelium / Q000201:enzymology / Q000473:pathology; D005881:Gingiva / Q000201:enzymology / Q000473:pathology; D005891:Gingivitis / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007958:Leukocyte Count; D018375:Neutrophil Activation; D009504:Neutrophils / Q000201:enzymology / Q000473:pathology; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D017622:Periodontal Attachment Loss / Q000201:enzymology / Q000473:pathology; D010518:Periodontitis / Q000201:enzymology / Q000473:pathology*; D013223:Statistics as Topic; D018709:Statistics, Nonparametric
D009569:Nitric Oxide; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II