Cell cycle dependence of boron uptake from two boron compounds used for clinical neutron capture therapy.
In neutron capture therapy, it is important that the boron is selectively uptaken by tumor cells. In the present study, we used flow cytometry to sort the cells in the G0/G1 phase and those in the G2/M phase, and the boron concentration in each fraction was measured with inductively coupled plasma atomic emission spectroscopy. The results revealed that sodium borocaptate and boronophenylalanine (BPA), were associated with higher rates of boron uptake in the G2/M than in the G0/G1 phase. However, the difference was more prominent in the case of BPA. The G2/M:G0/G1 ratio decreased as a function of exposure time in BPA containing culture medium, thereby indicating the cell cycle dependency of BPA uptake. Such heterogeneity of boron uptake by tumor cells should be considered for microdosimetry.
D000818:Animals; D001894:Borohydrides / Q000493:pharmacokinetics*; D001895:Boron / Q000378:metabolism*; D001896:Boron Compounds / Q000493:pharmacokinetics*; D016754:Boron Neutron Capture Therapy; D002453:Cell Cycle / Q000502:physiology*; D006224:Cricetinae; D005434:Flow Cytometry; D051379:Mice; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D051381:Rats; D013054:Spectrophotometry, Atomic; D013438:Sulfhydryl Compounds / Q000493:pharmacokinetics*; D014407:Tumor Cells, Cultured / Q000378:metabolism* / Q000473:pathology*
C436045:4-dihydroxyborylphenylalanine; D001894:Borohydrides; D001896:Boron Compounds; D013438:Sulfhydryl Compounds; C014651:mercaptoundecahydrododecaborate; D010649:Phenylalanine; D001895:Boron