title: Identification and characterization of a novel gene encoding a SEREX antigen in chronic myeloid leukaemia.
abstract: The novel gene encoding Clone 4, previously isolated through the Serological screening of recombinant expression cDNA library (SEREX) in chronic myeloid leukaemia (CML), was characterized. It is localized to chromosome 7 at q22, occupies approximately 4.5 kb of DNA and comprises 4 exons. Three single nucleotide polymorphisms and a 13 base deletion/insertion polymorphism have been identified. The mRNA transcript was approximately 2.4 kb and contained a long 5'-untranslated region of almost 1 kb before the candidate open reading frame. Northern blot analysis and multiple tissue expression array indicated a restricted normal tissue distribution. Our findings provide a platform for future investigations into the role of the gene product in CML.
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D015152:Blotting, Northern; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics; D009857:Oncogenes / Q000235:genetics; D011110:Polymorphism, Genetic
chemical_list: D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins