Possible physiological role of H19 RNA.
The product of the imprinted oncofetal H19 gene is an untranslated RNA of unknown function. With the human cDNA Atlas microarray, we detected differentially expressed genes modulated by the presence of H19 RNA. Many of the genes that are upregulated by H19 RNA are known to contribute to the invasive, migratory, and angiogenic capacities of cells. Moreover, we provided experimental data indicating that whereas H19 RNA did not have any growth advantage for the cells when cultured in 10% fetal calf serum, it did confer an advantage when cells were cultured in serum-poor medium. This observation can be explained in part by the inability of the H19-expressing cells to induce the cyclin-dependent kinase inhibitor p57(kip2) in response to serum stress. Our results favor the possible role of the H19 gene in promoting cancer progression, angiogenesis, and metastasis.
D001973:Bromodeoxyuridine; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D004396:Coloring Agents; D016895:Culture Media, Serum-Free / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27; D017931:DNA Primers / Q000737:chemistry; D020869:Gene Expression Profiling; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010078:Oxazines; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm; D022661:RNA, Untranslated / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism; D014966:Xanthenes
D018797:Cell Cycle Proteins; D004396:Coloring Agents; D016895:Culture Media, Serum-Free; D017931:DNA Primers; C094335:H19 long non-coding RNA; D010078:Oxazines; D018809:Proliferating Cell Nuclear Antigen; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm; D022661:RNA, Untranslated; D025521:Tumor Suppressor Proteins; D014966:Xanthenes; D050760:Cyclin-Dependent Kinase Inhibitor p27; C005843:resazurin; D008156:Luciferases; D001973:Bromodeoxyuridine