[Kinetic characteristics of enzymatic hydrolysis of complex protein substrates for preparing culture media].
Hydrolysis of a protein mixture from muscle and bone tissues with the enzymatic system from procine pancreatic cell suspension was studied. Kinetic constants and the values of activation energy were determined for individual processes of the release of 15 amino acids. The kinetic characteristics of the overall enzymatic hydrolysis calculated from analysis of the changes in concentrations of terminal amino groups were compared with the characteristics obtained while studying the accumulation patterns of individual amino acids.
D000596:Amino Acids / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism; D003470:Culture Media; D006868:Hydrolysis; D007700:Kinetics; D009132:Muscles / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*
D000596:Amino Acids; D003470:Culture Media; D011506:Proteins
Kineticheskie kharakteristiki fermentativnogo gidroliza slozhnykh belkovykh substratov dlia polucheniia pitatel'nykh sred.