title: 1,3-beta-D glucan binding protein (BGBP) from the white shrimp, Penaeus vannamei, is also a heparin binding protein.
abstract: Shrimp BGBP was purified as a 100 kDa glycoprotein by affinity chromatography using immobilised heparin. BGBP bound simple carbohydrates, glycosaminoglycans like heparin sulphate and glycoproteins, but it was unable to agglutinate erythrocytes. Using an ELISA-based microplate assay, it was shown that simple carbohydrates such as n-glucose and D-mannose are competitive inhibitors of heparin sulphate binding to BGBP. Based on these properties BGBP is considered as a new type of heparin binding protein.
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods / Q000662:veterinary; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005947:Glucose / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000378:metabolism*; D006384:Hemagglutination; D006493:Heparin / Q000378:metabolism*; D006494:Heparin Antagonists / Q000494:pharmacology; D006497:Heparan Sulfate / Q000378:metabolism; D037102:Lectins; D008358:Mannose / Q000494:pharmacology; D033561:Penaeidae / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D005947:Glucose; D006025:Glycosaminoglycans; D006493:Heparin; D006494:Heparin Antagonists; D006497:Heparan Sulfate; D037102:Lectins; D008358:Mannose; C037937:glucan-binding proteins