Implication of the lymphocyte-specific nuclear body protein Sp140 in an innate response to human immunodeficiency virus type 1.
The viral infectivity factor (Vif) of human immunodeficiency virus type 1 (HIV-1) neutralizes an unidentified antiviral pathway that occurs only in nonpermissive (NP) cells. Using a yeast two-hybrid screen of a human lymphocyte cDNA library, we identified several potential Vif partners. One, the nuclear body protein Sp140, was found specifically in all NP cells (n = 12 cell lines tested; P < or = 0.001), and HIV-1 infection induced its partial dispersal from nuclear bodies into cytosolic colocalization with Vif. Our results implicate Sp140 in a response to HIV-1 that may be related to or coordinated with the pathway that inactivates HIV-1 lacking vif.
D034961:Antigens, Nuclear / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D016346:Gene Products, vif / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D008214:Lymphocytes / Q000378:metabolism / Q000821:virology; D016296:Mutagenesis; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D020298:U937 Cells; D054320:vif Gene Products, Human Immunodeficiency Virus
D034961:Antigens, Nuclear; D016346:Gene Products, vif; C102470:SP140 protein, human; D014157:Transcription Factors; D054320:vif Gene Products, Human Immunodeficiency Virus