Isolation of an intertypic poliovirus capsid recombinant from a child with vaccine-associated paralytic poliomyelitis.
The isolation of a capsid intertypic poliovirus recombinant from a child with vaccine-associated paralytic poliomyelitis is described. Virus 31043 had a Sabin-derived type 3-type 2-type 1 recombinant genome with a 5'-end crossover point within the capsid coding region. The result was a poliovirus chimera containing the entire coding sequence for antigenic site 3a derived from the Sabin type 2 strain. The recombinant virus showed altered antigenic properties but did not acquire type 2 antigenic characteristics. The significance of the presence in nature of such poliovirus chimeras and the consequences for the current efforts to detect potentially dangerous vaccine-derived poliovirus strains are discussed in the context of the global polio eradication initiative.
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D002213:Capsid / Q000276:immunology; D002675:Child, Preschool; D003434:Crossing Over, Genetic; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009500:Neutralization Tests; D010243:Paralysis / Q000276:immunology / Q000821:virology*; D011051:Poliomyelitis / Q000276:immunology / Q000821:virology*; D017955:Poliovirus / Q000235:genetics* / Q000276:immunology; D011055:Poliovirus Vaccine, Oral / Q000009:adverse effects / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D013696:Temperature; D014407:Tumor Cells, Cultured; D014774:Virulence
D000914:Antibodies, Viral; D000956:Antigens, Viral; D011055:Poliovirus Vaccine, Oral