title: Protein and peptide degradation in the intestine of the common brushtail possum (Trichosurus vulpecula).
abstract: A protein (bovine serum albumin: BSA) and a peptide (luteinizing hormone releasing hormone: LHRH) were used to evaluate proteolytic activity in the intestine of common brushtail possums (Marsupiala, Trichosurus vulpecula). Luminal and mucosal extracts were isolated from the duodenum, jejunum, ileum, caecum, proximal colon and distal colon, their protein content assessed and specific activities in metabolising LHRH and BSA determined in vitro. The degradation of LHRH by luminal extracts was compared with that by the pancreatic enzymes, chymotrypsin, trypsin, and elastase. The protein concentration (microg x mg-1) of mucosal extract in the duodenum was higher ( P<0.05) than in the proximal colon, but that of luminal extracts did not differ significantly between regions. Proteolytic activity of luminal extracts was greater ( P<0.01) in the jejunum and ileum than in the hindgut. In the small intestine, proteolytic activity of luminal enzymes far exceeded that of mucosal enzymes ( P<0.05). All three pancreatic enzymes hydrolysed LHRH, but chymotrypsin had the greatest activity. This study has demonstrated that, in possums, proteolysis occurs primarily in the small intestine through luminal enzymes, with chymotrypsin playing a major role. The possum hindgut contributes little to the metabolism of peptides and proteins, identifying it as a potential site to target for their absorption following oral delivery.
mesh_terms: D000818:Animals; D002918:Chymotrypsin / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D005766:Gastrointestinal Contents / Q000737:chemistry; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D009893:Opossums / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology; D010447:Peptide Hydrolases / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D004798:Enzymes; D012710:Serum Albumin, Bovine; D007987:Gonadotropin-Releasing Hormone; D010447:Peptide Hydrolases; D002918:Chymotrypsin