Differential regulation of CD36 expression in antigen-presenting cells: Oct-2 dependence in B lymphocytes but not dendritic cells or macrophages.
In mice, three antigen-presenting cell types [B lymphocytes, macrophages and dendritic cells (DC)] express the scavenger receptor CD36. This molecule has been implicated in many important functions, including DC maturation and antigen presentation. In murine B cells, the CD36 gene requires the Oct-2 transcription factor for its expression. We previously found that B cells from Oct-2-null mice display defects in maturation, survival and proliferation. Here we have looked for a possible role for CD36 in B cells, but found that CD36 is dispensable for all responses tested. Although loss of CD36 did not directly affect B cell function, it did modulate slightly the isotype and level of IgG produced in vivo in naive mice, and IgM in Leishmania-infected mice. We also show that in DC and macrophages, CD36 expression is independent of Oct-2. We conclude that CD36 does not play a major role in B cell function, but that CD36 may contribute indirectly to humoral immunity through cells of the innate immune system.
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D000938:Antigen-Presenting Cells / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism*; D018955:CD36 Antigens / Q000235:genetics* / Q000502:physiology; D004268:DNA-Binding Proteins / Q000502:physiology*; D003713:Dendritic Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D018320:Leishmania major / Q000276:immunology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000378:metabolism*; D008562:Membrane Glycoproteins; D051379:Mice; D008810:Mice, Inbred C57BL; D050813:Octamer Transcription Factor-2; D027361:Organic Anion Transporters; D051117:Scavenger Receptors, Class A; D014157:Transcription Factors / Q000502:physiology*
D000913:Antibodies, Protozoan; D018955:CD36 Antigens; D004268:DNA-Binding Proteins; D008562:Membrane Glycoproteins; D050813:Octamer Transcription Factor-2; D027361:Organic Anion Transporters; C489206:Pou2f2 protein, mouse; D051117:Scavenger Receptors, Class A; D014157:Transcription Factors