Differential display analysis of gene expression in yeast.
RNA differential display (DD) is a powerful and straightforward method that employs random reverse-transcription polymerase chain reaction amplification of mRNA species with electrophoresis for comparative analysis of two or more transcriptomes. The small yeast genome represents a convenient model for studying basic functions of the eukaryotic genome and simultaneously provides valuable information towards further refinement of this technique. Several examples discussed below illustrate how DD coupled with classical yeast genetic approaches may be used for studying transcriptionally regulated genetic systems.
D015152:Blotting, Northern; D018797:Cell Cycle Proteins / Q000235:genetics; D017931:DNA Primers; D005544:Forecasting; D020869:Gene Expression Profiling; D011495:Histone-Lysine N-Methyltransferase; D008780:Methyltransferases; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins
D018797:Cell Cycle Proteins; D017931:DNA Primers; D029702:Schizosaccharomyces pombe Proteins; D008780:Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; C112744:clr4 protein, S pombe