Amygdala kindling induces upregulation of mRNA for NKCC1, a Na(+), K(+)-2Cl(-) cotransporter, in the rat piriform cortex.
GABA, the main inhibitory neurotransmitter in the brain, elicits a hyperpolarizing response by activation of the GABA(A)-receptor/chloride-channel complex under conditions of normal Cl(-) homeostasis. Thus the pathogenesis of epilepsy could involve an impairment of GABA(A)-receptor-mediated inhibition due to a collapse of the Cl(-) gradient. We examined the expression patterns of Cl(-) transporters and a Cl(-) channel in a rat amygdala-kindling model. Activity-dependent increases were observed in the mRNA for NKCC1, an inwardly-directed Cl(-) transporter, in the piriform cortex. This suggests that an increase in [Cl(-)](i) and a resultant reduction in GABAergic inhibition may occur in the kindled piriform cortex.
D000679:Amygdala / Q000378:metabolism* / Q000503:physiopathology; D000818:Animals; D002712:Chlorides / Q000378:metabolism; D004195:Disease Models, Animal; D004827:Epilepsy / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D005786:Gene Expression Regulation / Q000502:physiology; D007696:Kindling, Neurologic / Q000235:genetics; D008297:Male; D009433:Neural Inhibition / Q000235:genetics*; D009434:Neural Pathways / Q000378:metabolism* / Q000503:physiopathology; D009833:Olfactory Pathways / Q000378:metabolism* / Q000503:physiopathology; D011188:Potassium / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D028021:Sodium-Potassium-Chloride Symporters / Q000235:genetics*; D064506:Solute Carrier Family 12, Member 2; D009435:Synaptic Transmission / Q000502:physiology; D015854:Up-Regulation / Q000235:genetics*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D002712:Chlorides; D012333:RNA, Messenger; D011963:Receptors, GABA-A; C578879:Slc12a2 protein, rat; D028021:Sodium-Potassium-Chloride Symporters; D064506:Solute Carrier Family 12, Member 2; D005680:gamma-Aminobutyric Acid; D012964:Sodium; D011188:Potassium