In vitro studies on the activity of japanese kampo herbal medicines Oren-Gedoku-To (TJ-15) and Toki-Shakuyaku-San (TJ-23) as scavengers of free radicals.
Oren-Gedoku-To (TJ-15) inhibited enzymatically (NADPH or CumOOH) and non-enzymatically (Fe-ascorbate) induced lipid peroxidation in rat liver microsomes as assessed by TBA-reactive product accumulation. Toki-Shakuyaku-San (TJ-23) had little effect on either system. The protective effect of TJ-15 against lipid peroxidation was not dependent upon the presence of microsomal drug-metabolizing activity and could not be fully accounted for by its action on microsomal electron transfer, as evaluated by studying the kinetics of cytochrome c reduction. Both TJ-15 and TJ-23 reduced the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), indicating their free radical scavenging ability. The reactivity of TJ-15 was comparable with that of standard ascorbate, while the activity of TJ-23 was approximately 10 times lower. In a free solution TJ-15 and TJ-23 effectively scavenged OH* radicals, as indicated by the inhibition of ethylene production from 2-keto-4-methiolbutyric acid (KMBA), and O2*- anion radicals, as assessed by the inhibitory effect on the rate of nitro blue tetrazolium (NBT) reduction by the O2*- generating system xanthine/xanthine oxidase.
D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004579:Electron Transport / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000737:chemistry; D066298:In Vitro Techniques; D007501:Iron / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D016877:Oxidants / Q000737:chemistry; D018384:Oxidative Stress / Q000502:physiology; D010851:Picrates / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000737:chemistry; D014969:Xanthine Oxidase / Q000737:chemistry
D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D016877:Oxidants; D010851:Picrates; C061676:oren gedoku to; C413220:toki-shakuyaku-san; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron; D014969:Xanthine Oxidase; D009251:NADPH-Ferrihemoprotein Reductase; D001205:Ascorbic Acid