Method for determining nitrogenous heterocycle compounds in wine.
Qualitative and quantitative analyses of N-heterocycle compounds were conducted by using a liquid-liquid extraction followed by additional chemical silica gel purification and injection into a gas chromatographic (GC) column coupled to a nitrogen-phosphorus detector (NPD). The purified extract fraction was investigated by GC-NPD and by GC-mass spectrometry. The compounds identified and quantified in wines were 2,4,5-trimethyloxazole, 2,4-dimethylthiazole, and 4-methylthiazole by GC-NPD and both 2-acetylthiazole and 2-acetyl-2-thiazoline by GC-MS. The procedure was used to analyze different wines and gave reliable and reproducible results.
D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006571:Heterocyclic Compounds / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D011786:Quality Control; D015203:Reproducibility of Results; D013844:Thiazoles / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D006571:Heterocyclic Compounds; D013844:Thiazoles