Cytokine profile in Behçet's disease patients. Relationship with disease activity.
OBJECTIVE
To analyse proinflammatory Th1 and Th2 cytokines in patients with Behçet's disease (BD) in relation to disease activity.


METHODS
Forty-five BD patients (25 in active stage) were investigated with ELISA for estimation of cytokines levels. Furthermore, cytokines intracellular synthesis (IL-4 and IFN-gamma) of CD4+ T cells was studied.


RESULTS
Active and in remission BD patients showed increased serum levels of Th1 (IFN-gamma, IL-12) and Th2 (IL-4, IL-6 and IL-10) cytokines. Active BD was characterized by a higher increase of IL-6, IL-10 and a striking increase of IL-17, IL-18 and IFN-gamma, compared to remission RD. Upon in vitro stimulation, the percentages of CD4+ T cells containing IFN-gamma and CD40L were higher in active BD compared to healthy controls.


CONCLUSIONS
Our results suggest that the microenvironment of CD4+ T cells in active BD may induce the production of more cells committed to Th1 than in BD patients in remission and healthy controls.
D000328:Adult; D001528:Behcet Syndrome / Q000097:blood* / Q000276:immunology*; D016207:Cytokines / Q000032:analysis / Q000097:blood*; D018450:Disease Progression; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis / Q000097:blood; D016753:Interleukin-10 / Q000032:analysis / Q000097:blood; D018664:Interleukin-12 / Q000032:analysis / Q000097:blood; D020381:Interleukin-17 / Q000032:analysis / Q000097:blood; D020382:Interleukin-18 / Q000032:analysis / Q000097:blood; D015847:Interleukin-4 / Q000032:analysis / Q000097:blood; D015850:Interleukin-6 / Q000032:analysis / Q000097:blood; D008297:Male; D008875:Middle Aged; D018417:Th1 Cells / Q000737:chemistry / Q000276:immunology; D018418:Th2 Cells / Q000737:chemistry / Q000276:immunology
D016207:Cytokines; D020381:Interleukin-17; D020382:Interleukin-18; D015850:Interleukin-6; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma