title: Surfactant wound irrigation for the treatment of staphylococcal clinical isolates.
abstract: Deep wound infection involving an implanted biomaterial is a devastating complication in orthopaedic surgery. Two-thirds of such infections are monomicrobial and the most commonly isolated bacteria in human osteomyelitis and orthopaedic device infection are Staphylococcus aureus and Staphylococcus epidermidis. The purpose of the current study was to examine the effectiveness of the previously reported sequential surfactant irrigation protocol against human-isolated clinical strains of Staphylococcus aureus and Staphylococcus epidermidis in the rat model of orthopaedic implant contamination. The infectivity rate of human-isolated clinical strains of Staphylococcus aureus in a contaminated complex orthopaedic wound was reduced effectively by a sequential surfactant irrigation protocol. Also, in this model, the infectivity of Staphylococcus epidermidis was reduced by normal saline irrigation alone when compared with no irrigation. Therefore, the sequential surfactant irrigation protocol may represent an effective method of wound irrigation in monomicrobial Staphylococcus aureus orthopaedic implant contamination, and normal saline irrigation may suffice in cases of monomicrobial Staphylococcus epidermidis contamination. Additional studies are necessary to determine the clinical use of surfactant irrigation.
mesh_terms: D000818:Animals; D019643:Arthroplasty, Replacement / Q000009:adverse effects; D004195:Disease Models, Animal; D007595:Joint Prosthesis / Q000009:adverse effects; D016459:Prosthesis-Related Infections / Q000209:etiology / Q000382:microbiology* / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013203:Staphylococcal Infections / Q000209:etiology / Q000382:microbiology* / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000302:isolation & purification*; D013501:Surface-Active Agents / Q000627:therapeutic use*; D013530:Surgical Wound Infection / Q000209:etiology / Q000382:microbiology* / Q000517:prevention & control*; D007507:Therapeutic Irrigation
chemical_list: D013501:Surface-Active Agents