title: A single p450 allele associated with insecticide resistance in Drosophila.
abstract: Insecticide resistance is one of the most widespread genetic changes caused by human activity, but we still understand little about the origins and spread of resistant alleles in global populations of insects. Here, via microarray analysis of all P450s in Drosophila melanogaster, we show that DDT-R, a gene conferring resistance to DDT, is associated with overtranscription of a single cytochrome P450 gene, Cyp6g1. Transgenic analysis of Cyp6g1 shows that overtranscription of this gene alone is both necessary and sufficient for resistance. Resistance and up-regulation in Drosophila populations are associated with a single Cyp6g1 allele that has spread globally. This allele is characterized by the insertion of an Accord transposable element into the 5' end of the Cyp6g1 gene.
mesh_terms: D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002874:Chromosome Mapping; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D003634:DDT; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D017344:Genes, Insect; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000378:metabolism; D007438:Introns; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D014158:Transcription, Genetic; D019076:Transgenes
chemical_list: D029721:Drosophila Proteins; D007306:Insecticides; D003577:Cytochrome P-450 Enzyme System; D003634:DDT; C446928:Cyp6g1 protein, Drosophila