title: Effects of estradiol and its antagonist tamoxifen on motility and metabolism of human spermatozoa.
abstract: An investigation was conducted to evaluate the motility and metabolism of human spermatozoa incubated at 37 degrees Celsius for 3 hours in a krebs phosphate Ringer solution containing different concentrations of 17beta-estradiol and tamoxifen.  17beta-estradiol (17beta E2) stimulated significantly the motility of human spermatozoa.  The oxidative metabolism and the intracellular ATP concentrations are enhanced with 10-20 mcg/ml of 17beta E2; the lactate production remains unchanged however.  Tamoxifen, an antiestrogen, inhibited the motility of spermatozoa, and was toxic at high concentrations.  Its effects on the energetic metabolism is dose-dependent; it increases the oxidative metabolism and decreases the lactate production and intracellular ATP at 3 mcg/ml.
mesh_terms: D001695:Biology; D019411:Clinical Laboratory Techniques; D003933:Diagnosis; D004703:Endocrine System; D004958:Estradiol; D004967:Estrogens; D005069:Evaluation Studies as Topic; D005193:Family Planning Services; D005299:Fertility Agents; D005835:Genitalia; D005854:Germ Cells; D006728:Hormones; D010827:Physiology; D012098:Reproduction; D012102:Reproductive Control Agents; D013083:Sperm Transport; D013094:Spermatozoa; D013629:Tamoxifen; D014566:Urogenital System
chemical_list: D004967:Estrogens; D005299:Fertility Agents; D006728:Hormones; D012102:Reproductive Control Agents; D013629:Tamoxifen; D004958:Estradiol
keywords: Biology; Endocrine System; Estradiol--analysis; Estradiol--side effects; Estrogens; Evaluation; Examinations And Diagnoses; Family Planning; Fertility Agents; Genitalia; Germ Cells; Hormones; Laboratory Examinations And Diagnoses; Laboratory Procedures; Physiology; Reproduction; Reproductive Control Agents; Sperm Transport; Spermatozoa--analysis; Tamoxifen--analysis; Tamoxifen--side effects; Urogenital System