title: An economical and preparative orthogonal solid phase synthesis of fluorescein and rhodamine derivatized peptides: FRET substrates for the Staphylococcus aureus sortase SrtA transpeptidase reaction.
abstract: An economical and preparative-scale orthogonal solid-phase method of incorporating carboxyrhodamine and carboxyfluorescein fluorescence resonance energy transfer (FRET) probes site-specifically into synthetic peptide substrates for the S. aureus Sortase transpeptidase SrtA has been developed.
mesh_terms: D019881:Aminoacyltransferases / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins; D003546:Cysteine Endopeptidases; D004735:Energy Transfer; D019793:Fluorescein / Q000737:chemistry*; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D015336:Molecular Probe Techniques; D010455:Peptides / Q000138:chemical synthesis*; D010458:Peptidyl Transferases / Q000737:chemistry / Q000378:metabolism*; D012235:Rhodamines / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D007527:Isoenzymes; D010455:Peptides; D012235:Rhodamines; D019881:Aminoacyltransferases; C120542:sortase A; D010458:Peptidyl Transferases; D003546:Cysteine Endopeptidases; D019793:Fluorescein