A putative receptor for dengue virus in mosquito tissues: localization of a 45-kDa glycoprotein.
Dengue virus (DENV) infects target cells by attaching to various cell receptors, many of which are still unknown. In C6/36 cells (Aedes albopictus cell line), DENV-4 bound to two glycoproteins of 40 and 45 kDa, located on the cell surface. Preincubation of cells with polyclonal antibody against the 45-kDa protein specifically blocked DENV-4 infection of C6/36 cells. The antibody and purified DENV-4 detected the 45-kDa molecule in total extracts from eggs, larvae, and pupae as well as from the midgut, ovary, and salivary glands from adult-stage Aedes aegypti mosquitoes, whereas in malphigian tubules it was absent. This suggests that the distribution of the 45-kDa protein correlates with tissue tropism of DENV infection in mosquitoes. The 45-kDa molecule was not detected in Anopheles albimanus mosquito. The relevance of our findings is discussed from the pathogenetic and vector competence viewpoints.
D000330:Aedes / Q000378:metabolism* / Q000821:virology; D000818:Animals; D002460:Cell Line; D003716:Dengue Virus / Q000502:physiology*; D005260:Female; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*
D008562:Membrane Glycoproteins; D011991:Receptors, Virus