[L-Glutamate oxidase from Streptomyces cremeus 510 MGU: growth media optimization for the enhancement of the producer fermentative activity].
The investigation was devoted to culture conditions optimization aimed to maximum secretion of extracellular L-glutamate oxidase by Streptomyces cremeus 510 MGU. It was shown that Ca ions at the concentration 5-20 mM and 0.1% ammonium sulphate enhanced activity of extracellular enzyme to 4 folds. L-glutamate acid supplement had no effect on enzyme activity. Influence of some bivalent cations and aeration regimes on L-glutamate oxidase activity was investigated. Growth media optimization along with screening of active variants resulted with isolation of the strain with L-glutamate oxidase activity about 2 U/mL Rate of peroxide degradation in the presence of filtrated culture of S. cremeus was determined by chemiluminescence method.
D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis* / Q000378:metabolism; D000645:Ammonium Sulfate / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D003470:Culture Media; D005285:Fermentation; D018698:Glutamic Acid / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008163:Luminescent Measurements; D013237:Stereoisomerism; D013302:Streptomyces / Q000378:metabolism*
D003470:Culture Media; D018698:Glutamic Acid; D003300:Copper; D006861:Hydrogen Peroxide; D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase; D002122:Calcium Chloride; C029892:cupric chloride; D000645:Ammonium Sulfate
L-glutamatoksidaza iz Streptomyces cremeus 510 MGU: povyshenie fermentativnoĭ aktivnosti produtsenta putem optimizatsii fermentatsionnoĭ sredy.