title: Molecular analysis of de novo pyrimidine synthesis in solanaceous species.
abstract: The de novo synthesis of pyrimidine nucleotides in plants has been analysed on a molecular level with special focus on cDNA cloning and structure analysis of all genes involved and their expression pattern during development. The exhaustive cloning of all cDNAs resulted from screening with heterologous cDNAs or by using complementation strategies with Escherichia coli mutants and subsequent enzyme activity measurements. Southern hybridization and comparison with the Arabidopsis genome reveals plant specific aspects and a simple genomic organization of pyrimidine synthesis in plants, which is superimposed by the postulated, complex subcellular compartmentalization. Northern hybridization evinces coordinated expression of all genes under developmental control during tobacco leaf growth.
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D001221:Aspartate Carbamoyltransferase / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D000090722:Dihydroorotate Dehydrogenase; D004926:Escherichia coli / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005796:Genes / Q000235:genetics; D005816:Genetic Complementation Test; D018745:Genome, Plant; D008969:Molecular Sequence Data; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D011743:Pyrimidines / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D000090722:Dihydroorotate Dehydrogenase; D011743:Pyrimidines; D012333:RNA, Messenger; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D001221:Aspartate Carbamoyltransferase; D002222:Carbamoyl-Phosphate Synthase (Ammonia); C030986:pyrimidine