An LC/DAD-UV/MS method for the rapid detection of aristolochic acid in plant preparations.
A new method using direct on-line coupling between HPLC and UV-DAD/MS was developed for the rapid detection of aristolochic acid I in plant preparations. The use of both UV-DAD and MS detectors enables this method to be very selective. A ng range detection limit was determined for both UV and MS detections. A quantitative determination of aristolochic acid I in plant preparations was achieved using UV and MS detection. A thin layer chromatography assay was also developed for the preliminary detection of aristolochic acid I in complex mixtures.
D030022:Aristolochia; D034341:Aristolochic Acids / Q000737:chemistry* / Q000302:isolation & purification; D002845:Chromatography / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D014466:Ultraviolet Rays
D034341:Aristolochic Acids; D010936:Plant Extracts; C000228:aristolochic acid I