Investigation of cervical patency and uterine appearance in domestic cats by fluoroscopy and scintigraphy.
The cervical patency of six domestic female cats was monitored under sedation by infusion of contrast medium (Omnipaque) into the cranial vagina during early oestrus, mid-oestrus, late oestrus and interoestrus or a radiopharmaceutical ((99m)Tc-HSA) during mid- and interoestrus in a non-ovulatory oestrous cycle. The transport of the contrast medium or the radiopharmaceutical through the cervix and within the uterine horns was observed under fluoroscopy and with the aid of scintigraphy. In three of the queens, transcervical transport of contrast medium was demonstrated in all stages of oestrus, in one queen during mid-oestrus, late oestrus and 1 day after oestrus, and in two queens only during late oestrus. The relations between the cervical patency to the contrast medium and the oestrous behaviour, cornification of the vaginal cells and the serum oestradiol-17beta concentration were evaluated, and a relationship was found between the cervical patency and the degree of vaginal cornification. Transcervical transport of the radiopharmaceutical was observed in three queens during mid-oestrus. When the cervix was open, hysterography under a fluoroscope and hysteroscintigraphy were performed. The fluoroscopic and scintigraphic recordings revealed the patterns of the uterine contractions during oestrus in both ascending and descending directions, and the movement of the uterine contents back and forth between the uterine horns. The hysterograms were classified according to the shape of the uterine horns and the appearance of the endometrial lining. Spiral-shaped uterine horns with a smooth inner contour were observed in two queens, and a corkscrew appearance with irregular filling defects in the uterine lumen was shown in two queens that had developed subclinical cystic endometrial hyperplasia. These findings demonstrated that fluids or particles deposited in the cranial vagina of the cat can be transported into the uterus during some stages of the oestrous cycle. The fluoroscopic and scintigraphic techniques developed in this study may be further modified to permit more detailed studies of uterine contractile patterns and sperm transport in the feline female reproductive tract. Hysterography proved useful to diagnose uterine disease. The information on cervical patency is of value also for the development of techniques for artificial insemination in this species, and should be studied also in the ovulatory cycle.
D000818:Animals; D000829:Animals, Domestic; D002415:Cats / Q000033:anatomy & histology*; D002584:Cervix Uteri / Q000000981:diagnostic imaging / Q000502:physiology*; D003287:Contrast Media; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000502:physiology*; D005260:Female; D005471:Fluoroscopy / Q000662:veterinary; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary; D010060:Ovulation / Q000502:physiology; D011877:Radionuclide Imaging / Q000662:veterinary; D019275:Radiopharmaceuticals; D014599:Uterus / Q000033:anatomy & histology
D003287:Contrast Media; D019275:Radiopharmaceuticals; D004958:Estradiol