title: Enzymatic synthesis of D-glucosone 6-phosphate (D-arabino-hexos-2-ulose 6-(dihydrogen phosphate)) and NMR analysis of its isomeric forms.
abstract: D-Glucosone 6-phosphate (D-arabino-hexos-2-ulose 6-(dihydrogen phosphate)) was prepared from D-glucosone (D-arabino-hexos-2-ulose) by enzymatic conversion with hexokinase. The isomeric composition of D-glucosone 6-phosphate in aqueous solution was quantitatively determined by NMR spectroscopy and compared to D-glucosone. The main isomers are the alpha-anomer (58%) and the beta-anomer (28%) of the hydrated pyranose form, and the beta-D-fructofuranose form (14%).
mesh_terms: D006593:Hexokinase / Q000378:metabolism*; D007536:Isomerism; D007661:Ketoses / Q000096:biosynthesis* / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010710:Phosphates / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D007661:Ketoses; D010710:Phosphates; C474836:glucosone 6-phosphate (arabino-hexos-2-ulose 6-(dihydrogen phosphate)); C017022:glucosone; D006593:Hexokinase