title: Experimental results of preparing laser-shaped stromal implants for laser-assisted intrastromal keratophakia in extremely complicated laser in situ keratomileusis cases.
abstract: OBJECTIVE
  To evaluate the feasibility of laser-shaped stromal implants from a donor eye for correcting extreme high hyperopia with irregular astigmatism and an exceptionally thin corneal bed.
  
  
  METHODS
  Thirty-one fresh enucleated porcine eyes were used. The procedure was initiated with mechanical de-epithelialization followed by a lamellar cut with a microkeratome, resulting in a hinged flap (thickness 150 microm, diameter 9.5 mm). The cornea was photoablated with PRK treatment for +8.00 D sphere (hyperopic lenticule group) and -5.00 D sphere (myopic group) by a scanning spot excimer laser. A customized scanning software algorithm was used to create a circumferential cut with a 6.5-mm internal diameter. The lenticule was removed from the stromal bed and measured by a surface profiling system. A clinical case was performed on a patient with previous LASIK and highly irregular hyperopic astigmatism to verify the clinical utility of the experimental setup.
  
  
  RESULTS
  Under the microscope, lenticules seemed round, regular, and transparent. The average surface profile of porcine corneas demonstrated good parabolic shape with individual variations as large as 30 microm, probably due to the corneal size and curvature differences between the porcine eye and the human eye--for which the microkeratome is designed. The patient underwent a topography-guided treatment after laser-assisted intrastromal keratophakia (LAIK) and 1-month follow-up showed an increase of UCVA, BSCVA, and central corneal thickness.
  
  
  CONCLUSIONS
  The use of modern scanning-spot excimer lasers and microkeratomes enabled us to produce stromal lenticules of good quality, which might be acceptable to implant into a human eye.
mesh_terms: D000328:Adult; D000818:Animals; D001251:Astigmatism / Q000601:surgery; D003319:Corneal Stroma / Q000601:surgery*; D019781:Corneal Topography; D005260:Female; D006801:Humans; D006956:Hyperopia / Q000601:surgery*; D020007:Implants, Experimental; D020731:Keratomileusis, Laser In Situ / Q000379:methods*; D013552:Swine; D014792:Visual Acuity