Platelet-agglutinating protein p37 from a patient with thrombotic thrombocytopenic purpura has characteristics similar to prethrombin 2.
Thrombotic thrombocytopenic purpura (TTP) is characterized by widespread platelet thrombi in arterioles and capillaries. Unusually large or multimeric von Willebrand factor, as well as one or more platelet-agglutinating factors, have been implicated in the pathogenesis of TTP. But, the actual mechanisms of platelet agglutination have not been satisfactorily explained. Recent studies suggested the 37-kDa platelet-agglutinating protein (PAP) p37 to be partially responsible for the formation of platelet thrombi in patients with TTP. We studied mobility in SDS-PAGE, the sequence of N-terminal amino acid residues, DNA and antigenic characteristics of PAP p37, which might be related to the pathogenesis of TTP. PAP p37 was purified from the plasma of a 31-year-old male Korean patient with acute TTP. The findings are as follows: (1) We compared PAP p37 with thrombin through the use of SDS-PAGE, either with or without beta-mercaptoethanol. PAP p37 did not appear to be cleaved between the A- and B-chains of prethrombin 2. However, thrombin did cleave between those of prethrombin 2, but linked with disulfide bridge. (2) N-terminal 21 amino acid sequence of PAP p37 was T-F-G-S-G- E-A-D-X-G-L-R-P-L-F-E-K-K-S-L-E. It appeared to be identical to that of 285-305 amino acid residues of human prothrombin (prethrombin 2). (3) No prothrombin gene DNA mutation was revealed. (4) The antigenicity of PAP p37 was similar to thrombin, which was a result of the competitive binding against the anti-thrombin antibody. With these results, we conclude that PAP p37 has similar characteristics to prethrombin2.
D000328:Adult; D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002847:Chromatography, Agarose; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000737:chemistry* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D008623:Mercaptoethanol / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D011499:Protein Processing, Post-Translational; D017433:Protein Structure, Secondary; D011516:Prothrombin / Q000737:chemistry* / Q000235:genetics; D011697:Purpura, Thrombotic Thrombocytopenic / Q000097:blood* / Q000209:etiology; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
D001798:Blood Proteins; D004792:Enzyme Precursors; C046935:platelet agglutinating protein, thrombotic thrombocytopenic purpura; D008623:Mercaptoethanol; D011516:Prothrombin; C012007:prethrombins