Functional cloning using pFB retroviral cDNA expression libraries.
Retroviral cDNA expression libraries allow the efficient introduction of complex cDNA libraries into virtually any mitotic cell type for screening based on gene function. The cDNA copy number per cell can be easily controlled by adjusting the multiplicity of infection, thus cell populations may be generated in which >90% of infected cells contain one to three cDNAs. We describe the isolation of two known oncogenes and one cell-surface receptor from a human Burkitt's lymphoma (Daudi) cDNA library inserted into the high-titer retroviral vector pFB.
D016475:3T3 Cells / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009857:Oncogenes / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D019908:Proto-Oncogene Proteins c-raf / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051567:Proto-Oncogene Proteins c-vav; D019948:Receptors, Interleukin-4 / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics* / Q000378:metabolism*
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D011518:Proto-Oncogene Proteins; D051567:Proto-Oncogene Proteins c-vav; D019948:Receptors, Interleukin-4; D011994:Recombinant Proteins; C496618:VAV1 protein, human; C496619:Vav1 protein, mouse; D019908:Proto-Oncogene Proteins c-raf