title: C-terminal truncation of WT1 delays but does not abolish hematopoiesis in embryoid bodies.
abstract: The effect of mutations that truncate the WT1 protein on in vitro hematopoietic differentiation from embryonal stem cells has been examined by CFU-A assay, o-dianisidine staining for heme, and RT-PCR analysis of the expression of fetal and adult globins. In two independently isolated ES cell lines the mutations delay but do not abolish hematopoiesis. Analysis of replated CFU-A colonies indicates that the delay occurs prior to the formation of hematopoietic stem cells. The results demonstrate a role for WT1 at the onset of hematopoiesis.
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D019070:Cell Lineage; D003114:Colony-Forming Units Assay; D004622:Embryo, Mammalian / Q000166:cytology*; D006410:Hematopoiesis; D051379:Mice; D009154:Mutation; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D013997:Time Factors; D025721:WT1 Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D025721:WT1 Proteins