Iron status of infants fed low-iron formula: no effect of added bovine lactoferrin or nucleotides.
BACKGROUND
The appropriate level of iron fortification in infant formula remains undetermined.


OBJECTIVE
We compared hematologic indexes and iron-status indicators in infants who were either breast-fed or fed formula with concentrations of 2 or 4 mg Fe/L and evaluated the effects of providing part of the iron as bovine lactoferrin and of adding nucleotides.


METHODS
Healthy term infants were exclusively breast-fed (n = 16) or fed formula (n = 10-12) from age 4 +/- 2 wk to 6 mo. Anthropometric measures were taken monthly, and blood samples were taken at 1, 4, and 6 mo. Hematologic indexes; indicators of iron, zinc, and copper status; and erythrocyte fatty acids were assessed.


RESULTS
No significant differences in hematology or iron status were observed between groups at 4 and 6 mo of age. Although 34% of all infants had a hemoglobin concentration <110 g/L at 6 mo, the absence of iron deficiency or defective erythropoiesis suggests that this hemoglobin cutoff is too high for this age group. Neither the source or the concentration of iron in formula nor fortification with nucleotides had any significant effect on serum zinc or copper, and nucleotide fortification did not affect erythrocyte fatty acids.


CONCLUSIONS
A concentration of 1.6 mg Fe/L formula meets the iron requirement of healthy term infants aged </=6 mo, and providing more iron does not benefit iron stores. Fortification with bovine lactoferrin or nucleotides did not benefit either iron status or erythrocyte fatty acids. Additional studies are needed to establish age-appropriate cutoffs for iron deficiency and iron deficiency anemia in infancy.
D000818:Animals; D001724:Birth Weight; D001827:Body Height; D001942:Breast Feeding; D002417:Cattle; D004909:Erythrocyte Indices; D004910:Erythrocyte Membrane / Q000737:chemistry; D005227:Fatty Acids / Q000097:blood; D005293:Ferritins / Q000097:blood; D006801:Humans; D007223:Infant; D007225:Infant Food; D007231:Infant, Newborn; D007501:Iron / Q000097:blood*; D019266:Iron, Dietary / Q000008:administration & dosage*; D007781:Lactoferrin / Q000008:administration & dosage*; D009711:Nucleotides / Q000008:administration & dosage*; D009752:Nutritional Status; D011485:Protein Binding; D011990:Receptors, Transferrin / Q000097:blood
D005227:Fatty Acids; D019266:Iron, Dietary; D009711:Nucleotides; D011990:Receptors, Transferrin; D005293:Ferritins; D007501:Iron; D007781:Lactoferrin