Detection of Helicobacter colonization of the murine lower bowel by genus-specific PCR-denaturing gradient gel electrophoresis.
Helicobacter genus-specific PCR and denaturing gradient gel electrophoresis can detect and speciate the helicobacters that colonize the lower bowel of laboratory mice. The method's sensitivity is comparable to that of species-specific PCR and may detect unnamed Helicobacter species. This approach should prove useful for commercial and research murine facilities.
D000818:Animals; D002432:Cecum / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005243:Feces / Q000382:microbiology*; D016998:Helicobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity
D012336:RNA, Ribosomal, 16S