Determination of 5-methoxypsoralen in human serum.
A high performance liquid chromatography with fluorometric and ultraviolet detection described to quantify 5-methoxypsoralen (5-MOP) percutaneous absorption in humans after the application of an essential oil, as well as 5-MOP in bergamot oil and cosmetics by fluorometric and voltammetric measurement, respectively. A muBondapack C(18) analytical column (particle size 5 microm, 3.9 x 300 mm) eluted with acetonitrile-tetrahydrofuran-water (70:15:15, v/v/v) containing 0.07% trifluoroacetic acid. The quantification limits are 0.05 and 0.26 ng for 5-MOP and 5-geranoxypsoralen, respectively. For fluorometric measurement was found linear over the range 0.05-3.00 microg/ml for psoralens. Five volunteers blood samples were collected over a 2-day period were investigated before and after treatment with bergamot oil. Serum levels of 5-MOP were significantly increased from the 4 h after the application of bergamot essential oil. The hourly mean levels were significantly higher after the application of bergamot essential oil compared to baseline values.
D000078223:5-Methoxypsoralen; D000328:Adult; D004355:Drug Stability; D005260:Female; D006801:Humans; D008297:Male; D008730:Methoxsalen / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood* / Q000737:chemistry; D009822:Oils, Volatile / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D012869:Skin Absorption / Q000502:physiology
D009822:Oils, Volatile; D000078223:5-Methoxypsoralen; D008730:Methoxsalen