title: Primary cutaneous extragenital canine transmissible venereal tumour with Leishmania-laden neoplastic cells: a further suggestion of histiocytic origin?
abstract: The clinical signs and histopathological features of a primary extragenital canine transmissible venereal tumour (TVT) are described. Three subcutaneous round alopecic nodules were located on the anterior and caudal dorsal region and in the ventral area of the neck. Cytologically, tumour cells were intermediate in size with a moderate amount of cytoplasm, and the nuclei were immature with finely reticular chromatin. The cytoplasm was lightly to heavily basophilic and contained distinct small vacuoles at the periphery. On the basis of these characteristics, a diagnosis of TVT was made and confirmed by histological and ultrastructural investigations. Leishmania amastigotes were detected in the cytoplasm of macrophages and neoplastic cells of the tumoral mass. The presence of the parasite within neoplastic cells is consistent with a histiocytic origin of TVT.
mesh_terms: D000818:Animals; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000175:diagnosis* / Q000473:pathology; D004285:Dogs; D005260:Female; D007891:Leishmania / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D009333:Neck; D012878:Skin Neoplasms / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D014685:Venereal Tumors, Veterinary / Q000150:complications / Q000175:diagnosis*