G-protein-coupled receptor microarrays.
Membrane-bound proteins represent the single most important class of drug targets. Arraying these proteins is difficult because they typically need to be embedded in membranes to maintain their correctly folded conformations. We describe here the fabrication of microarrays consisting of G-protein-coupled receptors (GPCRs)--the single largest family of membrane-bound proteins-by robotic pin-printing on slides, and demonstrate assays for screening of ligands on these arrays.
D001667:Binding, Competitive; D005454:Fluorescence Polarization; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D009496:Neurotensin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D010446:Peptide Fragments / Q000378:metabolism; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011941:Receptors, Adrenergic / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D018028:Receptors, Neurotensin / Q000378:metabolism; D012235:Rhodamines / Q000737:chemistry / Q000494:pharmacology; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry; D013379:Substrate Specificity
D008024:Ligands; D008055:Lipids; D010446:Peptide Fragments; D011941:Receptors, Adrenergic; D011956:Receptors, Cell Surface; D018028:Receptors, Neurotensin; D012235:Rhodamines; D012821:Silanes; C042090:neuromedin N; D009496:Neurotensin; C024702:aminopropylsilane; D019204:GTP-Binding Proteins