Double-staining method for differentiation of morphological changes and membrane integrity of Campylobacter coli cells.
We developed a double-staining procedure involving NanoOrange dye (Molecular Probes, Eugene, Oreg.) and membrane integrity stains (LIVE/DEAD BacLight kit; Molecular Probes) to show the morphological and membrane integrity changes of Campylobacter coli cells during growth. The conversion from a spiral to a coccoid morphology via intermediary forms and the membrane integrity changes of the C. coli cells can be detected with the double-staining procedure. Our data indicate that young or actively growing cells are mainly spiral shaped (green-stained cells), but older cells undergo a degenerative change to coccoid forms (red-stained cells). Club-shaped transition cell forms were observed with NanoOrange stain. Chlorinated drinking water affected the viability but not the morphology of C. coli cells.
D017000:Campylobacter coli / Q000254:growth & development / Q000648:ultrastructure*; D002470:Cell Survival; D003470:Culture Media; D008854:Microscopy, Electron; D011933:Reagent Kits, Diagnostic / Q000382:microbiology; D013194:Staining and Labeling / Q000379:methods*
D003470:Culture Media; D011933:Reagent Kits, Diagnostic