Construction of a genetic linkage map of shiitake mushroom Lentinula edodes strain L-54.
From fruiting bodies of L. edodes strain L-54, single-spore isolates (SSIs) were collected. Two parental types of L-54 were regenerated via monokaryotization. By means of random-amplified polymorphic DNA (RAPD), DNA samples from L-54, its two parental types, and 32 SSIs were amplified with arbitrary primers. Dedikaryotization was demonstrated, and 91 RAPD-based molecular markers were generated. RAPD markers that were segregated at a 1:1 ratio were used to construct a linkage map of L. edodes. This RAPD-linkage map greatly enhanced the mapping of other inheritable and stable markers [such as those that are linked to a phenotype (the mating type), a known gene (priA) and a sequenced DNA fragment (MAT)] with the aid of mating tests, bulked-segregant analysis, and PCR-single-strand conformational polymorphism. These markers comprised a genetic map of L. edodes with 14 linkage groups and a total length of 622.4 cM.
D002874:Chromosome Mapping; D017931:DNA Primers; D005656:Fungal Proteins; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D005819:Genetic Markers; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D019105:Random Amplified Polymorphic DNA Technique; D020942:Shiitake Mushrooms / Q000235:genetics*
D017931:DNA Primers; D005656:Fungal Proteins; D005819:Genetic Markers; C075074:priA protein, Lentinus edodes