Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris.
The Pichia pastoris expression system offers economy, ease of manipulation, the ability to perform complex post-translational modifications, and high expression levels. Using this system, recent advances have been made in the quality of recombinant proteins in fermenter culture and in the quality of the protein product, namely improved secretion signals and glycosylation patterns.
D019149:Bioreactors; D002460:Cell Line; D017077:Culture Media, Conditioned; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D006031:Glycosylation; D006801:Humans; D010843:Pichia / Q000235:genetics* / Q000378:metabolism*; D011786:Quality Control; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D013045:Species Specificity
D017077:Culture Media, Conditioned; D011994:Recombinant Proteins