title: Cofortification of iron-fortified flour with zinc sulfate, but not zinc oxide, decreases iron absorption in Indonesian children.
abstract: BACKGROUND
  Iron deficiency is a major nutritional concern in developing countries, and food fortification is a common strategy to treat it. In Indonesia wheat flour is fortified with 60 mg Fe/kg, but because of increasing concerns about marginal zinc status in at-risk populations, consideration is being given to cofortifying flour with zinc. However, little is known about the effect of zinc fortification of flour on iron bioavailability or about the optimum form of zinc supplementation.
  
  
  OBJECTIVE
  We measured iron and zinc bioavailability from wheat-flour dumplings containing 25 g flour fortified with 60 mg Fe/kg, either alone or with 60 mg Zn/kg as zinc oxide or as zinc sulfate.
  
  
  METHODS
  Ninety children aged 4-8 y were recruited and assigned randomly to the 3 groups; 86 completed the study. Iron and zinc absorption were measured with established stable-isotope methods.
  
  
  RESULTS
  Iron absorption from the flour fortified with iron only was good (15.9 +/- 6.8%), but when corrections were made for hemoglobin concentrations, it was significantly lower from the flour cofortified with zinc sulfate (11.5 +/- 4.9%; P < 0.05) but not from the flour cofortified with zinc oxide (14.0 +/- 8.9%). Zinc absorption was not significantly different between the zinc oxide and zinc sulfate cofortified flours (24.1 +/- 8.2% compared with 23.7 +/- 11.2%; P = 0.87).
  
  
  CONCLUSIONS
  Iron and zinc appear to be highly bioavailable from foods made from fortified flour, but zinc sulfate cofortification may have a detrimental effect on iron absorption.
mesh_terms: D001682:Biological Availability; D002648:Child; D002675:Child, Preschool; D005260:Female; D005433:Flour; D005527:Food, Fortified; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007214:Indonesia; D007501:Iron / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D008297:Male; D015032:Zinc / Q000493:pharmacokinetics; D015034:Zinc Oxide / Q000008:administration & dosage*; D019287:Zinc Sulfate / Q000008:administration & dosage* / Q000009:adverse effects
chemical_list: D006454:Hemoglobins; D019287:Zinc Sulfate; D007501:Iron; D015032:Zinc; D015034:Zinc Oxide