The effect of carboxymethyl-chitosan on proliferation and collagen secretion of normal and keloid skin fibroblasts.
In this study, different molecular weight CM-chitosans were prepared and the effects on the growth and collagen secretion of normal skin fibroblasts and keloid fibroblasts were investigated in vitro. CM-chitosan promoted the proliferation of the normal skin fibroblast significantly but inhibited the proliferation of keloid fibroblast. The higher CM-chitosan concentration had a higher initial effect and the lower CM-chitosan concentration had a longer affecting time to the normal skin fibroblast. The lower molecular weight CM-chitosan had significant twofold activities. The CM-chitosan could reduce the ratio of type I/III collagen in keloid fibroblast by inhibiting the secretion of collagen type I; and had no effect on the secretion of types I and III collagen in the normal skin fibroblast.
D000328:Adult; D001672:Biocompatible Materials; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D048271:Chitosan; D003094:Collagen / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007627:Keloid / Q000378:metabolism*; D008970:Molecular Weight; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
D001672:Biocompatible Materials; D002686:Chitin; C068302:N-(carboxymethyl)chitosan; D003094:Collagen; D048271:Chitosan