Expression of cysteine peptidase cathepsin L and its inhibitors stefins A and B in relation to tumorigenicity of breast cancer cell lines.
The in vitro invasiveness of human breast cancer cell lines was compared with their reported tumorigenicity in vivo, increasing from MCF7, MDA-MB468, MDA-MB231 to MDA-MB435 cells. The invasiveness roughly corresponded to the tumorigenicity of the cell lines. The levels of cathepsin L mRNA and protein correlated with the invasiveness of the cells. Stefin A protein decreased with the invasiveness and the reported tumorigenicity, whereas stefin B protein was significantly lower in all MDA-MB lines compared with the least invasive and tumorigenic MCF7 line. Our results suggest that the imbalance between cathepsin L and the stefins contributes to the development of a malignant cell phenotype.
D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D056668:Cathepsin L; D002403:Cathepsins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D003094:Collagen / Q000737:chemistry; D055312:Cystatin A; D055313:Cystatin B; D015891:Cystatins / Q000235:genetics / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000235:genetics / Q000378:metabolism*; D018450:Disease Progression; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007797:Laminin / Q000737:chemistry; D009361:Neoplasm Invasiveness; D011509:Proteoglycans / Q000737:chemistry; D012334:RNA, Neoplasm / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism*
C524994:CSTB protein, human; D055312:Cystatin A; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D012334:RNA, Neoplasm; C059485:matrigel; C026407:CSTA protein, human; D055313:Cystatin B; D003094:Collagen; D002403:Cathepsins; D003546:Cysteine Endopeptidases; C534281:CTSL protein, human; D056668:Cathepsin L