The effect of three bufadienolide cardiac glycosides on contraction of isolated rat jejunum.
Three cardiac glycosides were screened for pharmacological effects on isolated rat jejunum. The contraction of rat jejunum with epoxyscillirosidin, a non-cumulative bufadienolide, and cotyledoside and tyledoside D, both cumulative neurotoxic bufadienolides were compared with methacholine. The results indicate that all three bufadienolides cause contraction of jejunal smooth muscle. When combined with atropine (1 x 10(-6) M) the response of epoxyscillirosidin and tyledoside D decreased, indicating suppression of a cholinergic response caused by the cardiac glycosides.
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002018:Bufanolides / Q000494:pharmacology*; D002301:Cardiac Glycosides / Q000494:pharmacology*; D002770:Cholenes / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007583:Jejunum / Q000187:drug effects*; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002018:Bufanolides; D002301:Cardiac Glycosides; D002770:Cholenes; D004791:Enzyme Inhibitors; C110052:epoxyscillirosidin; C469809:tyledoside D; D016210:Methacholine Chloride; C038322:cotyledoside; D001285:Atropine