Separation of salidroside from Rhodiola crenulata by high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) was used to purify salidroside from an extract of Rhodiola crenulata with two steps using a two-phase solvent system composed of ethyl acetate-n-butanol-water (1:4:5, v/v) in the first run and chloroform-methanol-isopropanol-water (5:6:1:4) in the second run. The method yielded 21.9 mg of salidroside from 1.216 g of the crude sample at 98% purity determined by HPLC analyses. Identification was performed by 1H NMR, 13C NMR, and MS.
D003377:Countercurrent Distribution / Q000379:methods*; D005960:Glucosides / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010636:Phenols / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D029771:Rhodiola / Q000737:chemistry*
D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; C009172:rhodioloside