title: Early, selective, and marked loss of sympathetic nerves from the islets of BioBreeder diabetic rats.
abstract: To discover whether islet sympathetic nerves are damaged during the autoimmune destruction of islet B-cells, we immunostained sections of pancreas from BioBreeder (BB) diabetic rats, using antibodies against vesicular monoamine transporter 2 (VMAT2), a marker of sympathetic nerve terminals. We found a marked decrease in the VMAT2-positive fiber area in the islets of BB rats that had been diabetic for only 1-2 weeks compared with their nondiabetic controls. In contrast, there was no significant decrease in the VMAT2-positive fiber area in the exocrine pancreas in these early diabetic BB rats. Furthermore, streptozotocin-diabetic rats showed no decrease in VMAT2-positive fiber area in their islets compared with controls. The classical diabetic autonomic neuropathy (DAN) that eventually occurs in the heart was not present in BB diabetic rats at this early stage as evidenced by normal cardiac VMAT2 immunostaining and normal cardiac norepinephrine content. Also, in contrast to DAN, this islet neuropathy did not worsen with duration of diabetes. These data provide evidence of a heretofore unrecognized early sympathetic islet neuropathy (eSIN). Because eSIN occurs selectively in the islet, is rapid in onset, and is associated with autoimmune but not chemically induced diabetes, it is distinct from DAN in location, time course, and mechanism.
mesh_terms: D000818:Animals; D000906:Antibodies; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000473:pathology*; D005260:Female; D007515:Islets of Langerhans / Q000294:innervation* / Q000473:pathology*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology; D026901:Membrane Transport Proteins; D009412:Nerve Fibers / Q000737:chemistry / Q000473:pathology; D009479:Neuropeptides; D016627:Oxidopamine; D010523:Peripheral Nervous System Diseases / Q000235:genetics / Q000473:pathology; D051381:Rats; D011913:Rats, Inbred BB; D013563:Sympathectomy, Chemical; D013564:Sympathetic Nervous System / Q000473:pathology*; D013565:Sympatholytics; D050492:Vesicular Biogenic Amine Transport Proteins; D050493:Vesicular Monoamine Transport Proteins
chemical_list: D000906:Antibodies; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009479:Neuropeptides; C493454:Slc18a2 protein, rat; D013565:Sympatholytics; D050492:Vesicular Biogenic Amine Transport Proteins; D050493:Vesicular Monoamine Transport Proteins; D016627:Oxidopamine