Expression of CTL1 in myelinating structures of Torpedo marmorata.
We initially isolated CTL1 from the electric lobe of brain through functional complementation of a yeast mutant deficient in choline transport. Here, we present the first characterization of an antibody to the C-terminal of CTL1. When full length torpedo CTL1 was expressed in oocytes, a broad 60 kDa band appeared concomitant with the detection of immunoreactivity at the plasma membrane. In, the native protein was prominent throughout the CNS and along the electric nerves. CTL1 immunolabeling was particularly conspicuous in the myelin sheath surrounding the electric nerve and in central myelinated structures. The association of the presumptive choline transporter, CTL1, with myelin membranes suggests a role for this new protein in lipid production.
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity / Q000276:immunology; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002794:Choline / Q000378:metabolism*; D004557:Electric Organ / Q000294:innervation / Q000378:metabolism; D004560:Electricity; D008563:Membrane Lipids / Q000096:biosynthesis*; D026901:Membrane Transport Proteins / Q000276:immunology / Q000378:metabolism*; D008853:Microscopy; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000502:physiology; D014101:Torpedo / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*
D000906:Antibodies; D008563:Membrane Lipids; D026901:Membrane Transport Proteins; C065637:choline transporter; D002794:Choline