Enhanced detection of viral hemorrhagic septicemia virus (a salmonid rhabdovirus) by pretreatment of the virus with a combinatorial library-selected peptide.
A 17-mer peptide (SAAEASAKATAEATAKG, p5) was selected by screening a combinatorial library for its ability to enhance in vitro the infectivity of viral hemorrhagic septicemia virus (VHSV), a salmonid rhabdovirus. Preincubation of VHSV samples with p5 at micromolar concentrations led to up to 5-fold increase of viral titers compared to untreated samples, as measured by a 1-day post-infection immunochemical focus assay. Treatment with p5 also increased VHSV titers when using the more traditional plaque and end-point dilution assays. Preincubation of p5 with infectious hematopoietic necrosis virus (another rhabdovirus of salmonids), but not with infectious pancreatic necrosis virus (birnavirus) also led to a similar increase in sensitivity. These results indicate that the addition of p5 may be used to improve the sensitivity of diagnostic tests for salmonid rhabdoviruses.
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002478:Cells, Cultured; D020650:Combinatorial Chemistry Techniques; D003530:Cyprinidae; D005393:Fish Diseases / Q000821:virology*; D008969:Molecular Sequence Data; D029141:Novirhabdovirus / Q000302:isolation & purification* / Q000472:pathogenicity*; D017686:Oncorhynchus mykiss; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits; D018353:Rhabdoviridae Infections / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity; D010948:Viral Plaque Assay
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D019151:Peptide Library; D010455:Peptides