Different unfolding pathways for mesophilic and thermophilic homologues of serine hydroxymethyltransferase.
To determine how much information can be transferred from folding and unfolding studies of one protein to another member of the same family or between the mesophilic and thermophilic homologues of a protein, we have characterized the equilibrium unfolding process of the dimeric enzyme serine hydroxymethyltransferase (SHMT) from two sources, Bacillus subtilis (bsSHMT) and Bacillus stearothermophilus (bstSHMT). Although the sequences of the two enzymes are highly identical ( approximately 77%) and homologous (89%), bstSHMT shows a significantly higher stability against both thermal and urea denaturation than bsSHMT. The GdmCl-induced unfolding of bsSHMT was found to be a two-step process with dissociation of the native dimer, resulting in stabilization of a monomeric species, followed by the unfolding of the monomeric species. A similar unfolding pathway has been reported for Escherichia coli aspartate aminotransferase, a member of the type I fold family of PLP binding enzymes such as SHMT, the sequence of which is only slightly identical ( approximately 14%) with that of SHMT. In contrast, for bstSHMT, a highly cooperative unfolding without stabilization of any monomeric intermediate was observed. These studies suggest that mesophilic proteins of the same structural family even sharing a low level of sequence identity may follow a common unfolding mechanism, whereas the mesophilic and thermophilic homologues of the same protein despite having a high degree of sequence identity may follow significantly different unfolding mechanisms.
D000595:Amino Acid Sequence; D001219:Aspartate Aminotransferases / Q000235:genetics; D001412:Bacillus subtilis / Q000378:metabolism; D002845:Chromatography; D002942:Circular Dichroism; D004795:Enzyme Stability; D005456:Fluorescent Dyes; D005976:Glutaral / Q000378:metabolism; D012696:Glycine Hydroxymethyltransferase / Q000235:genetics / Q000378:metabolism*; D019791:Guanidine / Q000378:metabolism; D006358:Hot Temperature; D008969:Molecular Sequence Data; D011489:Protein Denaturation / Q000502:physiology*; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011732:Pyridoxal Phosphate / Q000378:metabolism; D016415:Sequence Alignment; D017385:Sequence Homology; D014508:Urea / Q000378:metabolism
D005456:Fluorescent Dyes; D011732:Pyridoxal Phosphate; D014508:Urea; D012696:Glycine Hydroxymethyltransferase; D001219:Aspartate Aminotransferases; D019791:Guanidine; D005976:Glutaral