Cytotoxic effects of gossypol and vitamin E on human and rat lymphocytes and spermatozoa.
The cytotoxicity of vitamin E, gossypol, and mixture of both was determined in lymphocytes and spermatozoa using different concentrations of the substances.  The cellular viability was assessed by means of the trypan blue exclusion test at 0, 60, and 120 minutes.  Spermatozoa exhibited a higher sensitivity to these compounds, which was proportional to the length of exposure and the dose.
D001695:Biology; D002477:Cells; D019411:Clinical Laboratory Techniques; D003267:Contraception; D003270:Contraceptive Agents; D003272:Contraceptive Agents, Male; D003933:Diagnosis; D005193:Family Planning Services; D005835:Genitalia; D005854:Germ Cells; D006072:Gossypol; D006651:Histocytochemistry; D010827:Physiology; D013094:Spermatozoa; D014566:Urogenital System; D014815:Vitamins
D003270:Contraceptive Agents; D003272:Contraceptive Agents, Male; D014815:Vitamins; D006072:Gossypol
Biology; Contraception; Contraceptive Agents; Contraceptive Agents, Male; Cytochemical Effects; Cytologic Effects; Examinations And Diagnoses; Family Planning; Genitalia; Germ Cells; Gossypol; Laboratory Examinations And Diagnoses; Laboratory Procedures; Physiology; Spermatozoa; Urogenital System; Vitamins