title: Differential receptor usage of small ruminant lentiviruses in ovine and caprine cells: host range but not cytopathic phenotype is determined by receptor usage.
abstract: The ovine maedi-visna (MVV) and caprine arthritis-encephalitis (CAEV) small ruminant lentiviruses (SRLV) exhibit differential species tropism and cytopathic effects in vitro. Icelandic MVV-K1514 is a lytic SRLV which can infect cells from many species in addition to ruminants, whereas a lytic North American MVV strain (85/34) as well as nonlytic MVV strain S93 and CAEV can infect only ruminant cells. In the present study, we determined if differential receptor usage in sheep and goat cells is the basis of differential species tropism or cytopathic phenotype of SRLV. Infection interference assays in sheep and goat synovial membrane cells using pseudotyped CAEV vectors showed that North American MVV strains 85/34 and S93 and CAEV use a common receptor (SRLV receptor A), whereas MVV-K1514 uses a different receptor (SRLV receptor B). In addition, human 293T cells expressing CAEV but not MVV-K1514 envelope glycoproteins fused with a goat cell line persistently infected with MVV-K1514, indicating that MVV-K1514 does not use SRLV receptor A for cell-to-cell fusion. Therefore, our results indicate that the differential species tropism of SRLV is determined by receptor usage. However, receptor usage is unrelated to cytopathic phenotype.
mesh_terms: D000818:Animals; D016088:Arthritis-Encephalitis Virus, Caprine / Q000472:pathogenicity / Q000502:physiology*; D006041:Goats; D008561:Membrane Fusion; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D011991:Receptors, Virus / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012756:Sheep; D014759:Viral Envelope Proteins / Q000096:biosynthesis; D014762:Viral Interference; D014779:Virus Replication; D014790:Visna-maedi virus / Q000472:pathogenicity / Q000502:physiology*
chemical_list: D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins