Two-dimensional gel electrophoresis; better than a poke in the ICAT?
To date, the most widely used technology for conducting proteomic studies has been two-dimensional gel electrophoresis (2DGE), but this approach does have drawbacks. Isotope-coded affinity tagging (ICAT) is starting to challenge 2DGE as a new proteomic tool for the analysis of proteins in complex biological specimens. An appraisal of these two methodologies reveals that neither ICAT nor 2DGE provide comprehensive coverage on a proteome-wide scale.
D002846:Chromatography, Affinity / Q000379:methods* / Q000639:trends; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods* / Q000639:trends; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D046911:Macromolecular Substances; D025261:Mitochondrial Proton-Translocating ATPases / Q000032:analysis* / Q000378:metabolism; D010088:Oxidoreductases / Q000032:analysis* / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D011786:Quality Control
D046911:Macromolecular Substances; D011506:Proteins; D020543:Proteome; D010088:Oxidoreductases; D025261:Mitochondrial Proton-Translocating ATPases