Immunohistochemical characterization of lymphosarcoma in two alpacas (Lama pacos).
Species cross-reactive anti-peptide antibodies were assessed in formalin-fixed tissue for use in immunophenotyping of lymphosarcoma in two alpacas. Diagnosis of lymphosarcoma was made by routine histopathological examination. Primary antibodies used for immunophenotyping were anti-human CD3 and anti-human CD5 for T cells; and anti-human CD79a and anti-human CD79b for B cells/plasma cells. In one case, most of the neoplastic cells were labelled with both anti-CD3 and anti-CD79b, and smaller numbers were labelled with anti-CD79a. The other case was classified as a B-cell tumour on the basis of labelling of the majority of neoplastic cells with anti-CD79b and anti-CD79a. This is the first recorded attempt at immunophenotyping lymphosarcoma in alpacas and, to our knowledge, the first record of presumptive co-expression of T- and B-cell-associated molecules in lymphosarcoma in the veterinary literature.
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D001402:B-Lymphocytes / Q000737:chemistry / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis; D002161:Camelids, New World; D003429:Cross Reactions / Q000276:immunology; D017809:Fatal Outcome; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000737:chemistry / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000737:chemistry / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D008297:Male; D013601:T-Lymphocytes / Q000737:chemistry / Q000473:pathology
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor