An in vitro study of manure composition on the biochemical origins, composition, and accumulation of odorous compounds in cattle feedlots.
Very little is known about the biochemical origin of cattle feedlot odors and the environmental factors controlling their production. The tie between diet and manure composition is well established, but the effect of different manure compositions on odorous chemical production is unknown. This study describes the effect of starch, casein, and cellulose substrate additions to slurries of fresh (< 24 h) and aged cattle manure (> 1 d) on the anaerobic production of fermentation products and the consumption of substrates relative to no addition treatments. Aged cattle manure accumulated more VFA (245 to 290 mM) than the fresh manure (91 to 181 mM) irrespective of substrate additions (P < 0.001). In fresh manures, VFA concentrations were increased (P < 0.01) over no addition treatments when carbohydrate (starch or cellulose) was added, whereas starch and protein treatments to aged manure increased VFA content relative to no addition treatments (P < 0.001). Branched-chain VFA and aromatic compounds accumulated only in the aged manure (no addition and protein treatments), indicating that some protein fermentation occurred in those treatments. Based upon substrate loss, starch fermentation was the dominant process in both manures and all treatments with losses exceeding 18.6 g/L. Protein fermentation occurred only in the aged manure, specifically the no addition and protein treatments, when starch was no longer available. The production of odorous compounds from manure was controlled by substrate availability and pH, with pH related to lactate accumulation. We believe that calcareous soil and lactate-consuming microorganisms in the aged manure slurries minimized slurry acidification and resulted in greater accumulations of odorous products. Substrate additions had little effect on the overall accumulation of odor compounds in manure but had profound effects on odor compound composition. We propose that modifying cattle diets to limit starch and protein excretion would profoundly affect the production and accumulation of odor compounds in feedlots.
D000818:Animals; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002364:Caseins / Q000378:metabolism; D002417:Cattle; D002482:Cellulose / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007773:Lactates; D008372:Manure / Q000032:analysis / Q000382:microbiology*; D009812:Odorants / Q000032:analysis* / Q000517:prevention & control; D011506:Proteins / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*; D013997:Time Factors
D002364:Caseins; D005232:Fatty Acids, Volatile; D007773:Lactates; D008372:Manure; D011506:Proteins; D002482:Cellulose; D013213:Starch