Imipenem-EDTA disk method for differentiation of metallo-beta-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp.
Rapid detection of metallo-beta-lactamase (MBL)-producing gram-negative bacilli is necessary to prevent their dissemination. The method using a disk with imipenem plus 750 micro g of EDTA differentiated all MBL-producing pseudomonads, and the sensitivity and specificity for acinetobacters were 95.7 and 91.0%, respectively. The imipenem-EDTA disks were stable for 12 and 16 weeks at 4 and -20 degrees C, respectively.
D000150:Acinetobacter / Q000201:enzymology* / Q000302:isolation & purification; D004351:Drug Resistance / Q000502:physiology; D004492:Edetic Acid / Q000737:chemistry; D006801:Humans; D015378:Imipenem / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology* / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000378:metabolism*
D011933:Reagent Kits, Diagnostic; D015378:Imipenem; D004492:Edetic Acid; D001618:beta-Lactamases