title: The biotic ligand model and a cellular approach to class B metal aquatic toxicity.
abstract: The biotic ligand model (BLM) and a cellular molecular mechanism approach represent two approaches to the correlation of metal speciation with observed toxicity to aquatic organisms. The two approaches are examined in some detail with particular reference to class B, or soft metals. Kinetic arguments are presented to suggest situations that can arise where the BLM criterion of equilibrium between all metal species in the bulk solution and the biotic ligand may not be satisfied and what might the consequences be to BLM predictive capability. Molecular mechanisms of toxicity are discussed in terms of how a class B metal might enter a cell, how it is distributed in a cell, and how the cell might respond to the unwanted metal. Specific examples are given for copper as an organism trace essential metal, which is toxic in excess, and for silver, a non-essential metal. As class B metals all bind strongly to sulfur, regulation of these metals requires that all S(II-) species be accounted for in aquatic systems, even under oxic conditions.
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects* / Q000502:physiology; D008024:Ligands; D008670:Metals / Q000378:metabolism / Q000633:toxicity*; D008954:Models, Biological; D014867:Water / Q000032:analysis
chemical_list: D008024:Ligands; D008670:Metals; D014867:Water