Mammalian Rcd1 is a novel transcriptional cofactor that mediates retinoic acid-induced cell differentiation.
Rcd1, initially identified as a factor essential for the commitment to nitrogen starvation-invoked differentiation in fission yeast, is one of the most conserved proteins found across eukaryotes, and its mammalian homolog is expressed in a variety of differentiating tissues. Here we show that mammalian Rcd1 is a novel transcriptional cofactor and is critically involved in the commitment step in the retinoic acid-induced differentiation of F9 mouse teratocarcinoma cells, at least in part, via forming complexes with retinoic acid receptor and activation transcription factor-2 (ATF-2). In addition, antisense oligonucleotide treatment of embryonic mouse lung explants suggests that Rcd1 also plays a role in retinoic acid-controlled lung development.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000378:metabolism; D013724:Teratoma; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
C468527:CNOT9 protein, human; D011994:Recombinant Proteins; D014157:Transcription Factors; D014212:Tretinoin