title: The Drosophila BRM complex facilitates global transcription by RNA polymerase II.
abstract: Drosophila brahma (brm) encodes the ATPase subunit of a 2 MDa complex that is related to yeast SWI/SNF and other chromatin-remodeling complexes. BRM was identified as a transcriptional activator of Hox genes required for the specification of body segment identities. To clarify the role of the BRM complex in the transcription of other genes, we examined its distribution on larval salivary gland polytene chromosomes. The BRM complex is associated with nearly all transcriptionally active chromatin in a pattern that is generally non-overlapping with that of Polycomb, a repressor of Hox gene transcription. Reduction of BRM function dramatically reduces the association of RNA polymerase II with salivary gland chromosomes. A few genes, such as induced heat shock loci, are not associated with the BRM complex; transcription of these genes is not compromised by loss of BRM function. The distribution of the BRM complex thus correlates with a dependence on BRM for gene activity. These data suggest that the chromatin remodeling activity of the BRM complex plays a general role in facilitating transcription by RNA polymerase II.
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002843:Chromatin / Q000235:genetics; D002875:Chromosomes / Q000235:genetics / Q000648:ultrastructure; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D021122:Protein Subunits; D012319:RNA Polymerase II / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D018797:Cell Cycle Proteins; D002843:Chromatin; D029721:Drosophila Proteins; D021122:Protein Subunits; D015534:Trans-Activators; C086469:brm protein, Drosophila; D012319:RNA Polymerase II