Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells.
This study describes a reproducible cell culture system that permits the growth and secondary multiplication of the V4 strain of Newcastle disease virus. Allantoic fluid, magnesium chloride and diethylaminoethyl dextran were incorporated in Dulbecco's modified Eagle's medium to encourage secondary viral multiplication without adversely affecting healthy Madin Derby bovine kidney cell growth.
D000481:Allantoin / Q000378:metabolism; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002460:Cell Line; D003470:Culture Media / Q000032:analysis*; D003588:Cytopathogenic Effect, Viral; D003637:DEAE-Dextran / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000821:virology; D015636:Magnesium Chloride / Q000378:metabolism; D009522:Newcastle disease virus / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D014779:Virus Replication
D000956:Antigens, Viral; D003470:Culture Media; D015636:Magnesium Chloride; D000481:Allantoin; D003637:DEAE-Dextran