[Preliminary study on the gene expression profiles of oligodendrogliom as with cDNA array].
OBJECTIVE
To study the gene expression profiles of oligodendrogliomas with gene cDNA array.


METHODS
(32)P tagged cDNA probes converted from the total RNA, which had been extracted from 2 fresh samples of oligodendroglioma and 1 of normal brain tissue, were hybridized with the Atlas array. After washing the membranes, the autoradiography was performed and the autoradiograms were analyzed through the special software.


RESULTS
As compared to the normal brain tissue, there were 63 co-upregulated   genes and 4 co-downregulated genes in these 2 tumor samples. However, a significant quantitative difference existed between them. The expression trend of some genes differed from the known information.


CONCLUSIONS
cDNA array is effective for studying the gene expression profiles of oligodendrogliomas and provides new information for the further research on their molecular mechanisms.
D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D009837:Oligodendroglioma / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction