FT-IR study for hydroxyapatite/collagen nanocomposite cross-linked by glutaraldehyde.
FT-IR analysis was performed for the hydroxyapatite (HAp)/collagen (COL) nanocomposite cross-linked by glutaraldehyde (GA). The amide bands I, II and III from COL matrix, and phosphate and carbonate bands from HAp were identified. The amide B band arising from C-H stretching mode showed a sensitive conformation by the degree of cross-linking. The amide I band showed a complicate conformational change by the degree of cross-linking. The characteristic amide I band at 1685 cm(-1), which is known as an aging parameter in the biological bone, did not show a monotonous tendency by the degree of cross-linking. The relative contents of the organics in the cross-linked HAp/COL nanocomposite were evaluated as an integration ratio between the amide I band at 1600-1700 cm(-1) and PO(4)(3-) band at 900-1200 cm(-1). The increase of the organics content by the cross-linking is enabled by the further organization of Ca(2+) ions of HAp crystals in HAp/COL nanocomposite. The complicate conformational behavior in the amide I, II and III bands seems to be affected by the cross-linking induced directional arrangement of HAp/COL nanocomposite fibrils.
D000577:Amides / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D002254:Carbonates / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry*; D005976:Glutaral / Q000494:pharmacology*; D010710:Phosphates / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D000577:Amides; D001672:Biocompatible Materials; D002254:Carbonates; D003432:Cross-Linking Reagents; D010710:Phosphates; D003094:Collagen; D017886:Durapatite; D005976:Glutaral