title: The role of vagal efferents in regulation of gastric emptying and motility in rats.
abstract: Nonpharmacologic regulation of gastrointestinal motility may become competitive to actually applied methods in the nearest future. The Aim of this study was to evaluate effect of vagal stimulation on gastric motility and emptying. Experiments were performed on 30 male Wistar rats in vivo. Electrodes (120 um O Cr/Mo Microfil Industries) were placed on right vagal trunk below diaphragm without affecting its integrity. The fistula was implanted in gastric antrum. Stimulation parameters were: 0.3 V; 0.5 Hz, impulse duration--10 msec, time stimulation--5 min. Gastric pressure (balloon, Synectics pressure transducer, Sweden) and gastric emptying (red phenol method) were measured subsequently during and between stimulations.
  
  
  RESULTS
  Stimulation significantly decreased amplitude of gastric contractions about 14% (52.7 +/- +/- 24.5 vs control 66.8 +/- 15.0; p < 0.05) and increased liquid gastric emptying from isotonic solution about 10% (87.35 +/- 4.75 vs control 75.31 +/- 11.24; p < 0.001), hypertonic liquid about 15% (49.05 +/- 12.16 vs control 34.1 +/- 13.68; p < 0.001) and hypotonic liquid about 7% (83.05 +/- 8.8 vs control 76.29 +/- 11.88). The frequency of gastric contractions did not change significantly in Fast Fourier Analysis of the period of stimulations and in control group. CCK concentrations were not significantly different between stimulated and control group (0.3 +/- 0.08 vs control 0.27 +/- 0.06 pmol/L). L-NAME infusion abolished completely acceleration of gastric emptying of isotonic solution (50.38 +/- 12.66 vc control 87.82 +/- 5.49; p < 0.05), hypertonic solution (32.17 +/- 15.09 vs control 51.65 +/- 10.74; p < 0.05) and hypotonic solution (60.42 +/- 12.05 vs control 82.67 +/- 8.06; p < 0.05) during electrical stimulation.
  
  
  CONCLUSIONS
  In this experiment efferent stimulation of abdominal vagal nerve release neuromediators from afferent and efferent fibers. The main regulator seems to be nitric oxide. These results indicate the effective vagal nerve stimulation affects gastric motility and emptying. It is likely that observed effects reflect integrated response with activating vago-vagal reflexes and neurohumoral factors.
mesh_terms: D000818:Animals; D005583:Fourier Analysis; D005746:Gastric Emptying / Q000502:physiology*; D007262:Infusions, Intravenous; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000294:innervation*; D014630:Vagus Nerve / Q000502:physiology*
chemical_list: D019331:NG-Nitroarginine Methyl Ester