[Use of methylotrophic bacteria Methylobacillus flagellatum KT for isolation of deuterated exogenous carbohydrates].
The cultivation conditions optimal for biosynthesis of exogenous polysaccharides by methylotrophic bacteria Methylobacillus flagellatum were evaluated. The mutant strain most active in accumulating exogenous polysaccharides was selected. Gradual adaptation of this strain to the deuterated medium containing 1 vol % CD3OD in deuterium oxide intensified biosynthesis of exogenous polysaccharides and inhibited bacterial growth (compared to the standard medium). The monomeric composition of exogenous polysaccharides obtained during cultivation on standard and deuterated media was estimated by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy. Nondeuterated exogenous polysaccharides contained only fructose, while deuterated exogenous polysaccharides included 98% fructose and 2% ribose. Paramagnetic resonance spectroscopy showed that the degree of deuterium incorporation into molecules of biosynthetic carbohydrates was 89%.
D000222:Adaptation, Physiological; D002241:Carbohydrates / Q000096:biosynthesis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003903:Deuterium; D020631:Methylobacillus / Q000378:metabolism* / Q000502:physiology; D019906:Nuclear Magnetic Resonance, Biomolecular
D002241:Carbohydrates; D003903:Deuterium
Ispol'zovanie metilotrofnykh bakteriĭ Methylobacillus flagellatum KT dlia polucheniia deĭterirovannykh ékzogennykh uglevodov.