Evaluation of antibiotic sensitivity of Chlamydia trachomatis using RT-PCR.
The sensitivity of 11 clinical strains of Chlamydia trachomatis to azithromycin, ofloxacin, doxycycline, and erythromycin was evaluated. The minimum inhibiting concentrations of all antibiotics for 90% strains, determined by PCR with reverse transcription of omp3B gene RNA (GenBank U68443) corresponded to, and those with reverse transcription of 16S rRNA gene RNA (GenBank X54451) far surpassed the minimum bactericidal concentrations for 90% strains determined by direct immunofluorescence with monoclonal antibodies to the major outer membrane protein.
D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000187:drug effects* / Q000235:genetics; D017931:DNA Primers; D004352:Drug Resistance, Microbial; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D012329:RNA, Bacterial