title: Nine-year follow-up of a case of Grzybowski type multiple keratoacanthomas and failure to demonstrate human papillomavirus.
abstract: We describe a patient with a 9-year history of generalized eruptive keratoacanthoma (KA) of the Grzybowski type whose multiple skin lesions showed steady progression, resulting in a sclerotic, mask-like facial expression and ectropion. Eleven tumour biopsies representing lesions of different stages and localizations (erupting and regressing KAs, biopsies from non-involved light-protected and light-exposed skin, dermatosclerosis and squamous cell carcinomas) were analysed for human papillomavirus (HPV) sequences using a polymerase chain reaction approach capable of detecting the majority of all presently known HPV genotypes. None of the biopsy specimens proved to be HPV-positive, although HPV was detected in weakly and heavily affected control specimens by the method applied. These findings suggest an HPV-independent aetiology of this rare type of multiple KA.
mesh_terms: D000368:Aged; D018450:Disease Progression; D005153:Facial Neoplasms / Q000821:virology; D019066:Facies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007636:Keratoacanthoma / Q000821:virology*; D027383:Papillomaviridae / Q000302:isolation & purification*; D012878:Skin Neoplasms / Q000821:virology*