Effect of hot water extract from Agaricus blazei Murill on antibody-producing cells in mice.
We investigated the immunopotentiating activities of boiled water-soluble extracts from desiccated Agaricus blazei Murill (ABM). Effect of ABM extract on antibody production was investigated by method of hemolytic plaque-forming cells (PFC) against sheep red blood cells (SRBC) antigen. ABM extracts significantly (p<0.01) increased the number of PFC in spleen with intraperitoneal administration at doses of 25 mg/kg as compared with control group. The populations of Mac-1- or CD25-positive cells significantly (p<0.01, p<0.001) increased, but in CD19-positive cells, there were no differences in ABM-treated mice as compared with control mice. The expressions of IL-6 and IL-1beta mRNA were augmented by ABM extract in both peritoneal macrophages and spleen cells. These results suggested that ABM extract might be an effective stimulator for T cell and macrophage to IL-1beta and IL-6 release, resulting in augmentation of antibody production against SRBC antigen.
D000364:Agaricus; D000818:Animals; D000921:Antibody-Producing Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D005260:Female; D006358:Hot Temperature; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D014867:Water
D010936:Plant Extracts; D014867:Water