Expression of Interferon-gamma and tumour necrosis factor-alpha in pigs experimentally infected with Porcine Reproductive and Respiratory Syndrome Virus (PRRSV).
The expression of mRNA encoding interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) was studied, by reverse transcription-polymerase chain reaction and by in-situ hybridization with a non-radioactive digoxigenin-labelled probe, in formalin-fixed, paraffin wax-embedded lung tissue from pigs experimentally infected with a Korean isolate (North American genotype) of PRRSV. Both IFN-gamma and TNF-alpha mRNA were detected in the lung as early as 1 day post-inoculation (dpi) and up to 10 dpi. Hybridization signals for IFN-gamma and TNF-alpha were usually associated with inflammation, in particular with macrophages within alveolar septa. Expression of these cytokines was minimal in non-lesional lung of PRRSV-infected pigs and in normal lung from control pigs. The results suggest that IFN-gamma and TNF-alpha play a role in pulmonary defence against PRRSV.
D000818:Animals; D000831:Animals, Newborn; D017931:DNA Primers / Q000737:chemistry; D017403:In Situ Hybridization / Q000662:veterinary; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000378:metabolism / Q000473:pathology; D017563:Lung Diseases, Interstitial / Q000473:pathology / Q000662:veterinary / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000378:metabolism* / Q000473:pathology; D019316:Porcine respiratory and reproductive syndrome virus / Q000472:pathogenicity / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
D017931:DNA Primers; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma