The spectrin-ankyrin skeleton controls CD45 surface display and interleukin-2 production.
With T cell receptor stimulation, intracellular pools of CD45 and spectrin move to the surface. These processes are coupled. In both peripheral lymphocytes and Jurkat T cells, betaI spectrin and ankyrin associate with CD45. In Jurkat T cells, betaI spectrin peptides suppress surface recruitment of CD45 and CD3 and abrogate T cell activation. Other glycoproteins such as CD43 are not altered by the spectrin peptides. Spectrin's effects are mediated by ankyrin, which binds directly to the cytoplasmic domain of CD45 (K(d) = 4.3 +/- 3.0 nM). These data reveal a novel and unexpected contribution of the spectrin-ankyrin skeleton to the control of T lymphocyte function.
D017487:Ankyrins / Q000502:physiology* / Q000648:ultrastructure; D017252:CD3 Complex / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D019169:Jurkat Cells / Q000276:immunology / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000502:physiology*; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000502:physiology; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D011485:Protein Binding; D025941:Protein Interaction Mapping; D020033:Protein Isoforms / Q000502:physiology / Q000648:ultrastructure; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000502:physiology; D013049:Spectrin / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014162:Transfection
D017487:Ankyrins; D017252:CD3 Complex; D007376:Interleukin-2; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; C452439:SPTBN1 protein, human; D013049:Spectrin; D017493:Leukocyte Common Antigens