title: An ultracentrifugation analysis of two hundred fish genomes.
abstract: The goal of this study was to provide a comprehensive view of the compositional characteristics of fish genomes. We therefore expanded the number of fish species that we had explored so far in their DNAs by analytical ultracentrifugation in CsCl density gradient from 122 to 201. This study included representatives from three out of nine orders of Elasmobranchs (sharks and rays), both orders of dipnoan lungfishes, and both orders of chondrosteans (sturgeons and bichirs). We also studied 19 out of 38 teleostean orders, which represent all but four (minor) superorders of the subdivision Teleostei, a group comprising about 23,600 species (96% of all extant fishes). This leaves for further studies two subclasses, Holocephali (chimaeras), and Coelacanthimorpha (gombessas). In spite of this substantial increase in the number of species and orders analysed, all average properties (the modal buoyant density, rho(0), the average buoyant density, <rho>, the CsCl profile asymmetry, A, and the compositional heterogeneity, H), and all their ranges were unchanged compared to a previous study [J. Mol. Evol. 31 (1990) 265]. This suggests that, in all likelihood, the properties reported in the present paper can be considered as generally valid for all fish genomes.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D004247:DNA / Q000032:analysis / Q000235:genetics; D005399:Fishes / Q000235:genetics*; D016678:Genome
chemical_list: D002712:Chlorides; D002586:Cesium; D004247:DNA; C028019:cesium chloride