Early phenotypic changes of in vitro transformed cells during in vivo progression: possible role of the host innate immunity.
We described the earliest in vivo changes of in vitro transformed cells of various origin manifested in highly-increased H(2)O(2)-catabolizing and PGE(2)-releasing activities. We designate it as [H(2)O(2)(CA) + PGE(S)]-phenotype. It provided tumor cells with resistance to cytotoxicity of Mph and NK cells and correlated with increased tumorigenicity. Used as a functional marker of the early step of in vivo tumor progression [H(2)O(2)(CA) + PGE(S)]-phenotype allowed to estimate the difference in the rates of natural selection of tumor cells grown locally and/or disseminated and its significant delay related to bcl-2 gene expression. The data suggests that in vivo sublethal damage of tumor cells and activation of src gene are relevant to acquisition of [H(2)O(2)(CA) + PGE(S)]-phenotype.
D000818:Animals; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D006224:Cricetinae; D015232:Dinoprostone / Q000276:immunology / Q000378:metabolism*; D004622:Embryo, Mammalian; D019254:Genes, bcl-2 / Q000235:genetics; D006861:Hydrogen Peroxide / Q000276:immunology / Q000378:metabolism*; D007113:Immunity, Innate; D008647:Mesocricetus; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000209:etiology / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D009857:Oncogenes / Q000235:genetics; D010641:Phenotype; D014407:Tumor Cells, Cultured
D006861:Hydrogen Peroxide; D015232:Dinoprostone