Colorimetric SNP analysis using oligonucleotide-modified nanoparticles.
A point mutation in the p53 gene has been detected by means of fluorescence microscopy and fluorescent resonance energy transfer (FRET) through sequence selective aggregation of DNA-modified nanoparticles, in which fluorescent dyes were impregnated.
D001483:Base Sequence; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D004735:Energy Transfer; D005456:Fluorescent Dyes; D016158:Genes, p53 / Q000235:genetics*; D008856:Microscopy, Fluorescence / Q000379:methods; D015345:Oligonucleotide Probes / Q000737:chemistry*; D010316:Particle Size; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D004247:DNA