Comparative genomic hybridization analysis of PhIP-induced mammary carcinomas in rats reveals a cytogenetic signature.
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a mutagen/carcinogen belonging to the class of heterocyclic amines (HCAs) found in cooked meats, is a known rat mammary gland carcinogen. To gain insight into the genomic alterations associated with PhIP-induced carcinogenesis, we used comparative genomic hybridization (CGH) to examine chromosomal abnormalities in rat mammary gland carcinomas induced by PhIP. The alterations were compared to those induced by 7,12-dimethylbenz[a]anthracene (DMBA), a potent and well-studied mammary carcinogen. All six PhIP-induced carcinomas examined by CGH showed losses in the same specific regions of chromosomes 2, 3, 11, 18, and X, whereas three carcinomas induced by DMBA showed no consistent patterns of chromosomal gain or loss. This indicates that PhIP has a recognizable cytogenetic signature in rat mammary gland carcinomas.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D007093:Imidazoles / Q000633:toxicity*; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000235:genetics*; D051381:Rats
D002273:Carcinogens; D004273:DNA, Neoplasm; D007093:Imidazoles; D015127:9,10-Dimethyl-1,2-benzanthracene; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine