DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis with mitochondrial genes.
For DNA differential diagnosis of human Taenia cestodes, a base excision sequence scanning thymine-base method using the cytochrome c oxidase subunit I and cytochrome b genes as targets was used. The characteristic thymine-base peak profiles provide four distinct types, unique for T. saginata, T. asiatica, and two genotypes of T. solium. This approach provides a useful tool for the identification and diagnosis of human taeniid cestodes without DNA sequencing if nucleotide sequence databases are available.
D003573:Cytochrome b Group / Q000235:genetics; D018509:DNA, Helminth / Q000032:analysis*; D004272:DNA, Mitochondrial / Q000032:analysis*; D003576:Electron Transport Complex IV / Q000235:genetics; D006801:Humans; D041202:Taenia saginata / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D041201:Taenia solium / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology
D003573:Cytochrome b Group; D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV