Production of insoluble dextran using cell-bound dextransucrase of Leuconostoc mesenteroides NRRL B-523.
Water-insoluble, cell-free dextran biosynthesis from Leuconostoc mesenteroides NRRL B-523 has been examined. Cell-bound dextransucrase is used to produce cell-free dextran in a sucrose-rich acetate buffer medium. A comparison between the soluble and insoluble dextrans is made for various sucrose concentrations, and 15% sucrose gave the highest amount of cell-free dextran for a given time. L. mesenteroides B-523 produces more insoluble dextran than soluble dextran. The near cell-free synthesis was validated in a batch reactor, by monitoring the cell growth which is a small (10(6)-10(7) CFU/mL) and constant value throughout the synthesis.
D002021:Buffers; D002477:Cells / Q000201:enzymology*; D015169:Colony Count, Microbial; D003911:Dextrans / Q000096:biosynthesis* / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005285:Fermentation; D005964:Glucosyltransferases / Q000378:metabolism*; D007934:Leuconostoc / Q000145:classification* / Q000166:cytology / Q000201:enzymology*; D012995:Solubility; D013045:Species Specificity
D002021:Buffers; D003911:Dextrans; D004800:Enzymes, Immobilized; D005964:Glucosyltransferases; C020025:dextransucrase