Effect of N-tricyanovinylamines on the level of glutathione in hepatocytes.
The effects of N-substituted tricyanovinylamines (N-TCVA; RNHC(CN)=C(CN)2) have been studied on rat hepatocytes and liver mitochondria. Derivatives of N-TCVA act on oxidative phosphorylation as uncouplers, and react with thiols within pH 5.0-8.5. N-Isobutyl-, N-benzyl-, and N-cyclohexyl-TCVA influence the level of GSH and GSSG in isolated hepatocytes. They can act as oxidants, but the level of GSSG increases (about 40%) only if the concentration of N-TCVA is higher than 1 micromol/l. If N-TCVA is added to a final concentration higher than 50 micromol/l a decrease of GSH and GSSG level is observed. Derivatives of N-TCVA also influence the level of GSH and GSSG in mitochondria. At 40-400 micromol/l N-TCVA in the incubation medium the level of GSSG increased and the ratio GSH/GSSG was influenced, but the level of total SH groups did not decrease.
D000588:Amines / Q000737:chemistry*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014753:Vinyl Compounds / Q000494:pharmacology*
D000588:Amines; D009570:Nitriles; D014753:Vinyl Compounds; D005978:Glutathione