Cloning and characterization of the chick Oct binding factor OBF-1.
We have cloned the chicken homolog of OBF-1, chOBF-1, which comprises 256 amino acids, and exhibits only 65% overall identity to the human and mouse OBF-1 proteins. Amino acid sequence alignment revealed the putative Oct-binding sequence, RPYQGVRVKEPVKELL(K/R)RKRG, which is conserved among chicken, mouse and human. chOBF-1 protein was demonstrated to bind chicken Oct-1 protein by the in vitro immunoprecipitation experiment, and chOBF-1 was shown to functionally activate the chicken immunoglobulin (Ig) light chain promoter in the NIH 3T3 cell. Taken together, these data indicate that the Ig gene transcription machinery, including Oct-1 and OBF-1, has been highly conserved in vertebrate evolution.
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002645:Chickens; D004268:DNA-Binding Proteins / Q000737:chemistry; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D051863:Host Cell Factor C1; D051379:Mice; D008969:Molecular Sequence Data; D050811:Octamer Transcription Factor-1; D011233:Precipitin Tests; D016415:Sequence Alignment; D015534:Trans-Activators / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry
D004268:DNA-Binding Proteins; C497693:Hcfc1 protein, mouse; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; C494166:Pou2f1 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors