Correlation between the osmotic second virial coefficient and solubility for equine serum albumin and ovalbumin.
The Haas - Drenth - Wilson (HDW) (Haas et al., 1999) theoretical model was used to correlate osmotic second virial coefficient (B) values with solubility (S) values for equine serum albumin (ESA) and ovalbumin for corresponding solution conditions. The best fit from the theoretical model was compared to experimental S versus B data. B values were experimentally measured using static light scattering. Solubilities of ESA were estimated using a sitting drop method. When the experimental data for S versus B were plotted, an excellent fit for ESA was obtained according to the HDW model. The results showed that the coordination number (z) in the crystal lattice was 6, and the adjustable parameter (A) was 0.072. For ovalbumin, previously reported solubility data in aqueous ammonium sulfate solutions were utilized. The solubility data for ovalbumin were correlated with the measured B values obtained in our laboratory. The resulting best fit from the HDW model showed that z = 6 and A = 0.084.
D000645:Ammonium Sulfate; D000818:Animals; D016880:Anisotropy; D003460:Crystallization; D006736:Horses; D008027:Light; D008956:Models, Chemical; D008970:Molecular Weight; D009995:Osmosis; D010047:Ovalbumin / Q000737:chemistry*; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000737:chemistry*; D012995:Solubility
D012709:Serum Albumin; D010047:Ovalbumin; D000645:Ammonium Sulfate