Optimization of glycosidases production by Pseudoalteromonas issachenkonii KMM 3549(T).
OBJECTIVE
The present work aimed to design an optimized medium to yield a higher production of glycosides by Pseudoalteromonas issachenkonii KMM 3549(T).


RESULTS
Higher levels of fucoidan hydrolase, alginase, laminaranase and b-N-acetylglucosaminidase production were obtained with peptone concentrations ranging from 2.5 g l(-1) to 10 g l(-1), while the presence of both yeast extract and glucose did not affect enzyme production. The activity of fucoidan hydrolase and laminaranase increased up to 4.83 microM h(-1) mg(-1) and 19.23 microM h(-1) mg(-1) protein, respectively, in growth media containing xylose (1.0 g l(-1)), laminarin (0.5 g l(-1)) or alginate (0.5 g l(-1)), and production of b-N-acetylglucosaminidase substantially increased in the presence of fucoidan (0.5 g l(-1)) or galactose (1 g l(-1)). All polysaccharides tested in concentrations of 0.5 g l(-1) fucoidan and 0.2 g l(-1) fucose induced production of alginase (up to 5.06 microM h(-1) mg-1 protein).


CONCLUSIONS
The production of glycosidases is not only stimulated by the presence of algal polysaccharides, but may also be stimulated by monosaccharides (e.g. xylose).


CONCLUSIONS
The production of glycosidases by Pseudoalteromonas issachenkonii KMM 3549(T) was significantly improved by using a simple nutrient medium containing peptone (2.5 g l(-1)) and xylose (5.0 g l(-1)) in 100% natural seawater.
D003470:Culture Media; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D011133:Polysaccharide-Lyases; D011134:Polysaccharides / Q000378:metabolism*; D031161:Pseudoalteromonas / Q000201:enzymology* / Q000254:growth & development; D014994:Xylose / Q000008:administration & dosage / Q000378:metabolism*
D003470:Culture Media; D011134:Polysaccharides; D014994:Xylose; D006026:Glycoside Hydrolases; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase