Association between the cell cycle and neural crest delamination through specific regulation of G1/S transition.
Delamination of premigratory neural crest cells from the dorsal neural tube depends both upon environmental signals and cell-intrinsic mechanisms and is a prerequisite for cells to engage in migration. Here we show that avian neural crest cells synchronously emigrate from the neural tube in the S phase of the cell cycle. Furthermore, specific inhibition of the transition from G1 to S both in ovo and in explants blocks delamination, whereas arrest at the S or G2 phases has no immediate effect. Thus, the events taking place during G1 that control the transition from G1 to S are necessary for the epithelial to mesenchymal conversion of crest precursors.
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle / Q000502:physiology; D002454:Cell Differentiation; D002465:Cell Movement; D002467:Cell Nucleus / Q000502:physiology; D002642:Chick Embryo; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016193:G1 Phase / Q000502:physiology; D007701:Kinetin; D008898:Mimosine / Q000494:pharmacology; D008954:Models, Biological; D009024:Morphogenesis; D017570:MyoD Protein / Q000737:chemistry / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology / Q000196:embryology* / Q000502:physiology; D011687:Purines / Q000494:pharmacology; D011784:Quail / Q000196:embryology; D016196:S Phase / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism; D020032:Tyrphostins / Q000494:pharmacology
D000963:Antimetabolites; D004791:Enzyme Inhibitors; D017570:MyoD Protein; D011687:Purines; D014157:Transcription Factors; D020032:Tyrphostins; C095713:alpha-cyano-(3,4-dihydroxy)-N-(3-phenylpropyl)cinnamide; D008898:Mimosine; C090046:olomoucine; D004247:DNA; D001973:Bromodeoxyuridine; D007701:Kinetin