NRAMP1- or cytokine-induced bacteriostasis of Mycobacterium avium by mouse macrophages is independent of the respiratory burst.
Restriction of the growth of Mycobacterium avium was studied in wild-type and p47(phox)-deficient macrophages. The ability of gamma interferon and tumour necrosis factor alpha to induce antimycobacterial activity in bone-marrow-derived macrophages or the expression of the NRAMP1-mediated resistance to M. avium were not affected by the deficiency in p47(phox). The addition of exogenous iron increased mycobacterial growth in macrophages expressing a functional NRAMP1 protein or a mutant NRAMP1 protein. Reactive oxygen species are therefore not involved in the constitutive or induced anti-M. avium activities of the mouse macrophage.
D000818:Animals; D001853:Bone Marrow; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D009162:Mycobacterium avium / Q000254:growth & development* / Q000276:immunology; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D016897:Respiratory Burst / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D000099173:Natural Resistance-associated Macrophage Protein 1
D027682:Cation Transport Proteins; D007371:Interferon-gamma; D007501:Iron; D019255:NADPH Oxidases; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D000099173:Natural Resistance-associated Macrophage Protein 1