[Isolation of S-adenosyl-L-methionine from an enriched Kluyveromyces lactis mutant, grown in whey media].
The ability of six lactose-digesting yeast strains to accumulate S-adenosyl-L-methionine (AdoMet) when grown on whey medium was studied. Kluyveromyces lactis ATCC 8585 accumulated the greatest amount of AdoMet (15.2 mg per gram biomass) and was chosen for the development of an Ado-Met-enriched mutant. The Ado-Met-enriched mutant AM-65 was selected from mutants induced with N-methyl-N-nitro-N'-nitrosoguanidine. The strain accumulated 61.6 mg AdoMet per gram biomass. The effect of concentrations of medium components on AdoMet biosynthesis was studied. The ability of the mutant to accumulate AdoMet remained stable after multiple reinoculations.
D003470:Culture Media; D007716:Kluyveromyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008894:Milk Proteins; D009154:Mutation; D012436:S-Adenosylmethionine / Q000302:isolation & purification*; D000067816:Whey Proteins
D003470:Culture Media; D008894:Milk Proteins; D000067816:Whey Proteins; D012436:S-Adenosylmethionine
Vydelenie S-adenozyl-L-metionin--obogashchennogo mutanta Kluyveromyces lactis, vyrashchivaemogo na srede s syvrotkoĭ.