Separating nucleation and growth in protein crystallization using dynamic light scattering.
A means of controlling crystallization is to separate the phases of nucleation and growth. Methods to achieve this, other than seeding, involve lowering the supersaturation by changing the temperature or diluting drops after incubating them for a given time at nucleation conditions. However, by the time nuclei or crystals are visible under the microscope too many nuclei will have formed. Dynamic Light Scattering was applied practically, to determine the most likely time for nucleation-growth decoupling to be performed successfully. The time at which DLS showed a significant change in the size-distribution of species in solution, corresponded to that optimal time.
D000818:Animals; D003460:Crystallization / Q000379:methods*; D008027:Light; D010316:Particle Size; D010940:Plant Proteins / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012542:Scattering, Radiation; D012996:Solutions; D013549:Sweetening Agents / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry
D010940:Plant Proteins; D011506:Proteins; D012996:Solutions; D013549:Sweetening Agents; C003427:thaumatin protein, plant; D014357:Trypsin