Protein L mutants for the crystallization of antibody fragments.
In many cases, antibody and their complexes can be crystallized and their structure determined without major difficulties. The remaining problematic cases may be approached through techniques such as of combinatorial complex crystallization which uses immunoglobulin binding proteins (IBP). The range of lattices that can be made using this method can be expanded by engineering mutants of IBP domains. We have designed Peptostreptococcus magnus protein L (PpL) mutants with altered immunoglobulin light chain binding characteristics. While the wild type PpL has two binding sites, some of the mutants contact the light chain via only one site. Other mutants have combinations of weakened first and second binding sites that modify their crystallization properties and their packing mode. In this study, we have selected PpL mutants with different behavior and that are most useful for crystallization and we present the various packing modes obtained so far.
D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000936:Antigen-Antibody Complex / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics*; D001665:Binding Sites; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D046911:Macromolecular Substances; D051379:Mice; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010462:Peptostreptococcus / Q000737:chemistry / Q000235:genetics
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001426:Bacterial Proteins; C055047:Ig L-binding protein, Peptostreptococcus; D007140:Immunoglobulin Fab Fragments; D046911:Macromolecular Substances