title: Connexin-30 mRNA is up-regulated in astrocytes and expressed in apoptotic neuronal cells of rat brain following kainate-induced seizures.
abstract: Glial connexins (Cxs) make an extensively interconnected functional syncytium created by a network of gap junctions between astrocytes and oligodendrocytes. Among Cxs expressed in the brain, Cx30 is expressed in grey matter astrocytes, as shown at the protein level by immunoistochemistry. In the present study we aimed to perform a detailed study of the regional distribution of Cx30 mRNA in the adult and postnatal developing rat brain, analyzing its expression by in situ hybridization, and determining its cell type localization by double labeling. Recently, it has been suggested that neuronal activity may control the level of intercellular communication between astrocytes through gap junctions channels. Thus, a second aim of the present study was to investigate the short-term effects of kainate-induced seizures on Cx30 expression. The results showed that, in basal condition, Cx30 was expressed only in grey matter astrocytes with distinct regional patterns in developing and adult brain. Kainate treatment induced strong and region-specific changes of astroglial Cx30 mRNA levels and expression of Cx30 mRNA in neuronal cells undergoing cell death, suggesting a direct or indirect involvement of this connexin in the neuronal apoptotic process.
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000235:genetics*; D001253:Astrocytes / Q000378:metabolism*; D001921:Brain / Q000254:growth & development / Q000378:metabolism* / Q000503:physiopathology; D002450:Cell Communication / Q000235:genetics; D017630:Connexins / Q000235:genetics*; D018690:Excitatory Amino Acid Agonists; D015870:Gene Expression / Q000235:genetics*; D007150:Immunohistochemistry; D007608:Kainic Acid; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000235:genetics / Q000503:physiopathology; D013226:Status Epilepticus / Q000139:chemically induced / Q000235:genetics* / Q000503:physiopathology; D015854:Up-Regulation / Q000235:genetics
chemical_list: D017630:Connexins; D018690:Excitatory Amino Acid Agonists; D012333:RNA, Messenger; D007608:Kainic Acid