Fumonisin B1 affects viability and alters nitric oxide production of a murine macrophage cell line.
Fumonisin B1 (FB1), the major toxin produced by Fusarium verticillioides contaminating corn, is known to elicit many organ- and species-specific toxicities in animals. In the present study, exposure to FB1 decreased viability of a murine macrophage cell line (RAW264.7) in a dose-dependent manner (1-100 microM). Further, when cells exposed to FB1 were stimulated with lipopolysaccharide (LPS), a dose-dependent increase in production of nitric oxide (NO) was observed, but only at FB1 concentrations (10-50 microM) that induced significant cytotoxicity. Stimulation of cells with phorbol myristate acetate (PMA) resulted in increased NO production at 50 microM FB1, but induced a variable NO response at 1-10 microM FB1. Results suggest that FB1 affected cell viability and altered inducible NO production by RAW macrophages in a manner that was dependent on the pathway of stimulation.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D037341:Fumonisins / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis*
D037341:Fumonisins; D006131:Growth Inhibitors; D009569:Nitric Oxide; C056933:fumonisin B1