title: Karyotype and genome characterization in four cartilaginous fishes.
abstract: Different approaches can be used to elucidate the unsolved questions concerning taxonomic evolution in cartilaginous fish. The study of the karyological characteristics of these vertebrates by combining molecular and traditional techniques of chromosome preparation and banding has been demonstrated to be a very effective method. In this paper we studied the localization and the composition of the constitutive heterochromatin by using C- and restriction endonuclease-banding in four selachian species, belonging to two of the four superorders. We also characterized two different types of repetitive genomic sequences in these species: satellite DNA and (TTAGGG)(n) telomeric sequences. Finally, we analysed the nuclear ribosomal gene to determine the number of the nucleolar organizers and their position on chromosomes by using silver staining, chromomycin A(3), and FISH (fluorescent in situ hybridization). The results showed a prevailingly telomeric localization of constitutive heterochromatin in the Galeomorphii, the presence of additional nucleolar organizer sites in Raja asterias, an exclusively telomeric localization of the (TTAGGG)(n) sequences in Scyliorhinus stellaris and both telomeric and interstitial in Taeniura lymma. These data, together with those concerning the conservation of the satellite DNA, seem to support the hypothesis that Chondrichthyes have an evolutionary history leading them to the acquisition of large genomes rich in highly repeated sequences and subjected to some selective pressures favoring the conservation of this DNA fraction.
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004276:DNA, Satellite / Q000235:genetics; D004284:Dogfish / Q000235:genetics; D018940:Elasmobranchii / Q000235:genetics*; D016678:Genome; D006570:Heterochromatin / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D012754:Sharks / Q000235:genetics; D012860:Skates, Fish / Q000235:genetics; D016615:Telomere / Q000235:genetics
chemical_list: D004276:DNA, Satellite; D006570:Heterochromatin; D004247:DNA; D004262:DNA Restriction Enzymes