title: Germ cell lineage from a single blastomere at 8-cell stage in shiro-uo (ice goby).
abstract: Shiro-uo (ice goby; teleost fish), Leucopsarion petersii, shows a unique cleavage pattern characterized by two tires of blastomeres at 8-cell stage, like that of echinoderm and amphibian embryo. Such a pattern is suitable to isolation and cell lineage experiments. In this study, cell lineage of germ-line was traced by histological observation and cell labelling experiment at the 8-cell stage. Primordial germ cells (PGCs) were first detected histologically at the 10-somite stage, and migrated to gonadal anlage at 10 days post-fertilization, through usual way described in other teleost species. When a single blastomere was labelled with tracer dye at 8-cell stage, both upper and lower tires generated labelled PGCs at gonadal anlage although upper tires occasionally. This result suggests that all blastomeres at the 8-cell stage have potential to produce PGCs in shiro-uo.
mesh_terms: D000818:Animals; D001757:Blastomeres / Q000166:cytology*; D019070:Cell Lineage; D002465:Cell Movement; D004396:Coloring Agents; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000196:embryology; D005260:Female; D005399:Fishes / Q000196:embryology*; D005854:Germ Cells / Q000166:cytology*; D008297:Male; D013194:Staining and Labeling
chemical_list: D004396:Coloring Agents