title: Induction of apoptotic cellular death in lymphatic tissues of cattle experimentally infected with different strains of rinderpest virus.
abstract: The presence, type, and extent of cellular death in lymphatic tissues of cattle experimentally infected with rinderpest virus strains of different virulence was investigated morphologically. Cells with DNA strand breaks were identified in histological sections of palatine tonsil, spleen, and mesenteric and mandibular lymph nodes by the TUNEL (terminal desoxynucleotidyl transferase-mediated dUTP nick end labelling) assay. In addition, representative samples of lymphatic tissues were examined by transmission electron microscopy. The results indicated that cellular disassembly in lymphatic tissues was caused by both apoptosis and oncosis. Cells with DNA strand breaks were observed in follicular and parafollicular areas of lymphatic tissues and their numbers were determined. A significant correlation was found between the number of TUNEL-positive cells and viral virulence. These results suggest that, in addition to oncosis, apoptotic cellular death in lymphatic tissues contributes substantially to the pathogenesis of rinderpest.
mesh_terms: D000818:Animals; D017209:Apoptosis; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology* / Q000821:virology; D002452:Cell Count; D022082:Cellular Structures / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D020287:In Situ Nick-End Labeling / Q000662:veterinary; D008221:Lymphoid Tissue / Q000473:pathology* / Q000821:virology; D012301:Rinderpest / Q000209:etiology / Q000473:pathology* / Q000821:virology; D012302:Rinderpest virus / Q000145:classification / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D013045:Species Specificity
chemical_list: D004247:DNA