title: Effects of GABA(A) and glycine receptor agonists on the medullary inspiratory neuronal activity during spontaneous augmented breaths in anesthetized rats.
abstract: To clarify whether GABAergic or glycinergic transmission alters the activity of inspiratory neurons during spontaneous augmented breaths, we recorded the single unit activity from inspiratory neurons in the dorsal and ventral respiratory groups in the medulla of pentobarbital anesthetized rats and applied GABA(A) and glycine receptor agonists by iontophoresis using multibarrel microelectrodes. The spontaneous augmented breath was divided into two different phases; the first phase (phase I) resembled a normal inspiration but the second phase (phase II) indicated a marked increase in diaphragm electromyogram activity. During application of either muscimol or glycine, the discharge of inspiratory neurons during the phase I of spontaneous augmented breaths was suppressed, but the augmenting discharge of the phase II did not change significantly in any cell type of the neurons (I-augmenting, I-decrementing and I-other). These results suggested that the excitatory inputs to inspiratory neurons during the phase II of augmented breaths may not be significantly influenced by the activation of either GABA(A) receptors or glycine receptors.
mesh_terms: D000758:Anesthesia; D000818:Animals; D004594:Electrophysiology; D005110:Extracellular Space / Q000502:physiology; D018755:GABA Agonists / Q000008:administration & dosage / Q000494:pharmacology; D058785:GABA-A Receptor Agonists; D005998:Glycine / Q000008:administration & dosage / Q000494:pharmacology; D007478:Iontophoresis; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009118:Muscimol / Q000008:administration & dosage / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D018009:Receptors, Glycine / Q000819:agonists*; D015656:Respiratory Mechanics / Q000187:drug effects / Q000502:physiology*
chemical_list: D018755:GABA Agonists; D058785:GABA-A Receptor Agonists; D018009:Receptors, Glycine; D009118:Muscimol; D005998:Glycine