Crystallization of RNA/protein complexes.
Different complexes of ribosomal proteins with specific rRNA fragments have been crystallized and studied by our group during the last six years. There are several factors important for successful crystallization of RNA/protein complexes, among them: length and content of RNA fragments, homogeneity of RNA and protein preparations, stability of the complexes, conditions for mixing RNA and protein components before crystallization, effect of Se-Met on RNA/protein complex crystal quality. In this paper we describe findings and methodical details, which helped us to succeed in obtaining X-ray quality crystals of several RNA/protein complexes.
D001426:Bacterial Proteins / Q000737:chemistry; D001483:Base Sequence; D003460:Crystallization / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D046911:Macromolecular Substances; D017017:Methanococcus / Q000737:chemistry / Q000235:genetics; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000737:chemistry* / Q000235:genetics; D012269:Ribosomal Proteins / Q000737:chemistry*; D013696:Temperature; D016963:Thermus thermophilus / Q000737:chemistry / Q000235:genetics; D013997:Time Factors
D001426:Bacterial Proteins; D046911:Macromolecular Substances; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C106697:protein CTC, Bacteria; C029742:ribosomal protein L5; C036004:ribosomal protein S15; C030254:ribosomal protein S8