Immunoprecipitation and isoelectric focusing of sheep MHC class I antigens reveal higher complexity than serology.
Biochemical and serological methods were used to characterize sheep MHC class I polymorphism at the product level. The cells of 65 selected animals were subjected to immunoprecipitation and one-dimensional isoelectric focusing (IEF) with two cross-reacting monoclonal antibodies, B1.1G6 and HC-10. We were able to define up to 18 distinct haplotypes in the Latxa breed sample. Most of the locally defined serological specificities were confirmed or subdivided by biochemical typing. As IEF detected antigens not yet defined by serology, it provided us with a guide to producing more specific antisera by appropriate immunizations. Lastly, evidence of expression of two class I loci products was found.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005260:Female; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000276:immunology; D007525:Isoelectric Focusing; D011110:Polymorphism, Genetic; D011233:Precipitin Tests; D012698:Serologic Tests; D034561:Sheep, Domestic / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I