title: Dermatome setting for autografts to cover INTEGRA.
abstract: Using the INTEGRA Dermal Regeneration Template requires the outer silastic layer to be replaced with an autograft. We followed the manufacturer's directions for epidermal autografting and frequently obtained shredded, useless grafts, therefore, it seemed important to determine the proper dermatome setting. We evaluated dermatome settings from 0.002 to 0.012 inches. First, with feeler gauges, we verified the dermatome settings. Second, we harvested skin at various dermatome settings and measured the thickness histologically. We found that 1) the dermatome settings are reasonably accurate; 2) harvesting useful sheets at 0.002 and 0.004 inches is virtually impossible; 3) the variability of histologic graft thickness is enormous; and 4) a dermatome setting of 0.006 inches yields useful grafts. We no longer use the term epidermal autografting but rather ultrathin split-thickness grafting. To harvest these grafts, we now merely set the dermatome to 0.006 inches and make whatever midcourse corrections are necessary to obtain translucent grafts.
mesh_terms: D000328:Adult; D001672:Biocompatible Materials / Q000592:standards* / Q000627:therapeutic use*; D002056:Burns / Q000473:pathology / Q000628:therapy*; D002809:Chondroitin Sulfates; D002983:Clinical Competence / Q000592:standards; D003094:Collagen; D004817:Epidermis / Q000473:pathology / Q000637:transplantation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D016038:Skin Transplantation / Q000473:pathology / Q000592:standards*; D020858:Tissue and Organ Harvesting / Q000592:standards*; D014182:Transplantation, Autologous / Q000473:pathology / Q000592:standards*
chemical_list: D001672:Biocompatible Materials; C114835:integra artificial skin; D002809:Chondroitin Sulfates; D003094:Collagen