title: Local release of human neutrophil lipocalin (HNL), IL-8, and TNF-alpha is decreased as response to topical prednisolone treatment in distal ulcerative colitis and proctitis.
abstract: The local release of human neutrophil lipocalin, considered to be highly specific for neutrophil granulocyte activation, and interleukin-8 and tumor necrosis factor-alpha were studied in 11 patients with distal ulcerative colitis and proctitis before and during treatment with steroid enemas. A rectal perfusion technique for sampling and specific immunoassays for analysis were used. In responders (N = 8) the concentrations of all proteins decreased during the study. There was a close correlation between human neutrophil lipocalin concentrations and treatment response. Tumor necrosis factor-alpha showed an initial decline in concentrations irrespective of treatment outcome and preceded the decline of human neutrophil lipocalin and interleukin-8. We conclude that decreased neutrophil degranulation is correlated with treatment outcome. Furthermore, an important role of tumor necrosis factor-alpha in the process of stimulating neutrophil activation and degranulation in ulcerative colitis is suggested.
mesh_terms: D000209:Acute-Phase Proteins; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002352:Carrier Proteins / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000378:metabolism; D004733:Enema; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D000071068:Lipocalin-2; D054834:Lipocalins; D009504:Neutrophils / Q000378:metabolism*; D015513:Oncogene Proteins; D011239:Prednisolone / Q000008:administration & dosage / Q000627:therapeutic use*; D011349:Proctitis / Q000188:drug therapy* / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D000209:Acute-Phase Proteins; D000893:Anti-Inflammatory Agents; D002352:Carrier Proteins; D016209:Interleukin-8; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D014409:Tumor Necrosis Factor-alpha; D011239:Prednisolone