title: Receptors for recombinant feline interferon-omega in hemocytes of the Japanese pearl oyster Pinctada fucata martensii.
abstract: In a previous study we determined that administration of recombinant feline interferon-omega (rFeIFN-omega) could protect Japanese pearl oysters Pinctada fucata martensii from akoya-virus infection. Our results suggested that rFeIFN-omega enhanced the potential of agranulocytes to phagocytize necrotic cells and to produce collagen fibers that repair the lesions caused by the akoya-virus. In the present study, using an indirect immunofluorescence technique with an anti-rFeIFN-omega rabbit serum, we examined whether hemocytes bear receptors that bind rFeIFN-omega. rFeIFN-omega receptors were present on agranulocytes and bound rFeIFN-omega, and appeared as green fluorescent spots in the cytoplasm under a fluorescent microscope. Around 56% of agranulocytes in Japanese pearl oysters bore the rFeIFN-omega receptors.
mesh_terms: D000818:Animals; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006434:Hemocytes / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D006651:Histocytochemistry / Q000662:veterinary; D007370:Interferon Type I / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007564:Japan; D008856:Microscopy, Fluorescence / Q000662:veterinary; D010125:Ostreidae / Q000276:immunology / Q000378:metabolism; D017471:Receptors, Interferon / Q000378:metabolism*
chemical_list: D007370:Interferon Type I; D017471:Receptors, Interferon; C063743:interferon omega 1