title: Expression of MMP-8 and MMP-13 mRNAs in rat periodontium during tooth eruption.
abstract: The present study was designed to investigate mRNA expression of matrix metalloproteinase-8 (MMP-8) and MMP-13 in forming periodontium during tooth eruption in the rat. RT-PCR for the decalcified paraffin sections indicated expression of MMP-8 and MMP-13 in the periodontal tissues. In situ hydridization demonstrated expression of MMP-8 in osteoblasts, osteocytes, periodontal ligament cells, cementoblasts, and cementocytes along with collagen types I and III. In contrast, transcripts of MMP-13 were confined to a small population of osteoblasts and osteocytes in alveolar bone. The results suggested that MMP-8 may be involved in remodeling the periodontium during tooth eruption, and its expression may be coordinated with that of collagen types I and III, whereas the participation of MMP-13 may be rather limited.
mesh_terms: D000539:Alveolar Process / Q000166:cytology / Q000378:metabolism; D000818:Animals; D003094:Collagen / Q000032:analysis / Q000235:genetics; D024042:Collagen Type I / Q000032:analysis / Q000235:genetics; D000090602:Collagen Type I, alpha 1 Chain; D024061:Collagen Type III / Q000032:analysis / Q000235:genetics; D017364:Collagenases / Q000032:analysis* / Q000235:genetics; D003739:Dental Cementum / Q000378:metabolism; D015870:Gene Expression; D017403:In Situ Hybridization; D008297:Male; D053509:Matrix Metalloproteinase 13; D020784:Matrix Metalloproteinase 8 / Q000032:analysis* / Q000235:genetics; D010006:Osteoblasts / Q000378:metabolism; D010011:Osteocytes / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism; D010519:Periodontium / Q000378:metabolism*; D011498:Protein Precursors / Q000032:analysis / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014078:Tooth Eruption / Q000235:genetics / Q000502:physiology*
chemical_list: D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D024061:Collagen Type III; D011498:Protein Precursors; D015347:RNA Probes; D012333:RNA, Messenger; D003094:Collagen; D017364:Collagenases; D053509:Matrix Metalloproteinase 13; C507083:Mmp13 protein, rat; D020784:Matrix Metalloproteinase 8