Physical descriptions of experimental selectivity measurements in ion channels.
Three experiments that quantify the amount of selectivity exhibited by a biological ion channel are examined with Poisson-Nernst-Planck (PNP) theory. Conductance ratios and the conductance mole fraction experiments are examined by considering a simple model ion channel for which an approximate solution to the PNP equations with Donnan boundary conditions is derived. A more general result is derived for the Goldman-Hodgkin-Katz permeability ratio. The results show that (1) the conductance ratio measures the ratio of the diffusion coefficients of the ions inside the channel, (2) the mole fraction experiment measures the difference of the excess chemical potentials of the ions inside the channel, and (3) the permeability ratio measures both diffusion coefficients and excess chemical potentials. The results are used to divide selectivity into two components: partitioning, an equilibrium measure of how well the ions enter the channel, and diffusion, a nonequilibrium measure of how well the ions move through the channel.
D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology; D002463:Cell Membrane Permeability / Q000502:physiology*; D003198:Computer Simulation; D004553:Electric Conductivity; D004563:Electrochemistry / Q000379:methods*; D004594:Electrophysiology / Q000379:methods; D015640:Ion Channel Gating / Q000502:physiology; D007473:Ion Channels / Q000737:chemistry* / Q000502:physiology*; D017136:Ion Transport / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D008954:Models, Biological; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013816:Thermodynamics
D007473:Ion Channels