Impact of nasopharyngeal swab types on detection of Bordetella pertussis by PCR and culture.
A study was designed to assess the performance of various swabs and transport media routinely used to collect specimens submitted for Bordetella culture and PCR. Calcium-alginate swabs inhibited the PCR. No inhibition was detected in any PCRs with dacron or rayon swabs. All swab materials performed similarly for recovery of Bordetella pertussis in culture. The Amies with charcoal transport system performed poorly for culture. Calcium-alginate swabs are not recommended for PCR-based detection of B. pertussis. Dacron and rayon swabs are an excellent choice for both PCR and culture.
D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014917:Whooping Cough / Q000382:microbiology