Stimulation of N-methyl-D-aspartate (NMDA) receptors inhibits neuronal migration in embryonic cerebral cortex: a tissue culture study.
We performed a tissue culture study using embryonic rat brain to investigate the effect of excessive stimulation of NMDA receptors on neuronal migration in the cerebral cortex. After progenitor cells in the ventricular zone of E16 cerebral cortex explants were labeled with [(3)H]thymidine, the explants were cultured for 48 h, and distributions of labelled cells were evaluated autoradiographically. Stimulation of NMDA receptors by NMDA and ibotenate, NMDA receptor agonists added to the culture medium in separate studies, caused percentages of labeled cells to decrease significantly in the intermediate zone and increase in the ventricular zone. The results suggest that in rat cerebral cortex, agonist stimulation of NMDA receptors inhibits neuronal migration.
D000818:Animals; D001345:Autoradiography; D002465:Cell Movement / Q000502:physiology; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology; D046508:Culture Techniques; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity; D005260:Female; D007051:Ibotenic Acid / Q000633:toxicity; D008938:Mitosis / Q000187:drug effects; D016202:N-Methylaspartate / Q000633:toxicity; D009474:Neurons / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists*; D013234:Stem Cells / Q000502:physiology; D013936:Thymidine / Q000378:metabolism
D018690:Excitatory Amino Acid Agonists; D016194:Receptors, N-Methyl-D-Aspartate; D007051:Ibotenic Acid; D016202:N-Methylaspartate; D013936:Thymidine