Effects of endothelin-1 on DNA synthesis and NADPH diaphorase activity in epithelial and smooth muscle cells of the tracheobronchial system in newborn albino rats after repeated treatment with NG-nitro-L-arginine methyl ester.
Newborn rats received intraperitoneal injections of endothelin-1 in a daily dose of 5 x 10(-8)mol/kg 30 min after administration of NG-nitro-L-arginine methyl ester (9.3 x 10(-5)mol/kg intraperitoneally) from the 2nd to 6th day of life. NADPH diaphorase activity in epithelial and smooth muscle cells of the tracheobronchial system increased 24 h after the last treatment. In this period DNA synthesis underwent opposite changes, which included the inhibition of epithelial cell proliferation and stimulation of smooth muscle cell division. Intensification of free radical oxidation in the lung tissue was accompanied by inactivation of the antioxidant system.
D000818:Animals; D000831:Animals, Newborn; D001980:Bronchi / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D019332:Endothelin-1 / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D051381:Rats; D014132:Trachea / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism
D019332:Endothelin-1; D004791:Enzyme Inhibitors; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase; D019331:NG-Nitroarginine Methyl Ester