title: Severe IgE-mediated anaphylaxis following consumption of fried frog legs: definition of alpha-parvalbumin as the allergen in cause.
abstract: BACKGROUND
  IgE-mediated allergic reactions to bullfrog and edible frog have been reported. The implicated allergens have not been defined so far. The frog material and the patient's serum from a case of severe food-induced anaphylaxis were used to define the implicated allergen at the protein and DNA level.
  
  
  METHODS
  Immunoblotting techniques and N-terminal protein microsequencing were used to define the allergen recognized by the patient's serum. Back translation from the identified protein sequence was used to design degenerated primers to amplify the allergen's cDNA by polymerase chain reaction (PCR). We defined the nucleotide sequence of the allergen from the frog of Indonesian origin that was consumed by the patient, and the homologous cDNA from Rana esculenta.
  
  
  RESULTS
  Protein microsequencing revealed that the implicated frog allergen belonged to the parvalbumin family. cDNAs coding for alpha- and beta-parvalbumin of R. esculenta and Rana species were cloned. Recombinant proteins were expressed in Escherichia coli. The patient's serum IgE antibodies recognized parvalbumin prepared from frog muscle and recombinant alpha-parvalbumin from R. species but not from R. esculenta. Recombinant beta-parvalbumin was not recognized by the IgE antibodies.
  
  
  CONCLUSIONS
  This work defines at the protein and DNA levels alpha-parvalbumin as the allergen implicated in a case of IgE-mediated anaphylaxis to frog muscle. It also shows that a protein belonging to the parvalbumin family is implicated in type I allergies outside the fish species.
mesh_terms: D000485:Allergens / Q000009:adverse effects* / Q000032:analysis; D000595:Amino Acid Sequence; D000707:Anaphylaxis / Q000175:diagnosis / Q000209:etiology*; D000818:Animals; D001001:Anura; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000209:etiology*; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008461:Meat Products / Q000009:adverse effects* / Q000032:analysis; D008875:Middle Aged; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010320:Parvalbumins / Q000737:chemistry; D020539:Sequence Analysis, Protein; D012720:Severity of Illness Index
chemical_list: D000485:Allergens; D010320:Parvalbumins