title: Mechanisms of shock wave induced endothelial cell injury.
abstract: OBJECTIVE
  Medical procedures, for example, laser angioplasty and extracorporeal lithotripsy as well as high-energy trauma expose human tissues to shock waves (SWs) that may cause tissue injury. The mechanisms for this injury, often affecting blood vessel walls, are poorly understood. Here we sought to assess the role of two suggested factors, viz., cavitation or reactive oxygen species (ROS).
  
  
  METHODS
  A laser driven flyer-plate model was used to expose human umbilical cord vein endothelial cell (HUVEC) monolayers to SWs or to SWs plus cavitation (SWC). Cell injury was quantified with morphometry, trypan blue staining, and release of (51)Cr from labeled HUVECs.
  
  
  RESULTS
  HUVECs, exposed to SWs only, could not be distinguished from controls in morphological appearance or ability to exclude trypan blue. Yet, release of (51)Cr, indicated a significant cell injury (P < 0.05). HUVEC cultures exposed to SWC, exhibited cell detachment and cell membrane damage detectable with trypan blue. Release of (51)Cr was fourfold compared to SW samples (P < 0.01). Signs of cell injury were evident at 15 minutes and did not change over the next 4 hours. No protective effects of ROS scavengers were demonstrated.
  
  
  CONCLUSIONS
  Independent of ROS, SWC generated an immediate cell injury, which can explain, for example, vessel wall perturbation described in relation to SW treatments and trauma.
mesh_terms: D004730:Endothelium, Vascular / Q000293:injuries* / Q000503:physiopathology* / Q000528:radiation effects; D004847:Epithelial Cells / Q000502:physiology / Q000528:radiation effects*; D019220:High-Energy Shock Waves / Q000009:adverse effects*; D006801:Humans; D066298:In Vitro Techniques; D008954:Models, Biological; D017382:Reactive Oxygen Species / Q000009:adverse effects*; D015599:Trauma Severity Indices; D014471:Umbilical Veins / Q000293:injuries / Q000503:physiopathology / Q000528:radiation effects
chemical_list: D017382:Reactive Oxygen Species