title: [RNA isolation and purification methods].
abstract: Recent advances in human, bacterial and viral genome projects and the development of quantitative real-time reverse transcription-polymerase chain reaction methods offer the possibility of analysing a large number of gene transcripts. These molecular developments represent an important advancein the field of genetics, cancer, virology, bacteriology and hematology. A limiting step remains the isolation of high quality mRNA purified from biological samples. This review describes the different methods used to isolate mRNA from biological samples and to verify RNA integrity and gives precise details about RNA storage conditions.
mesh_terms: D015152:Blotting, Northern / Q000379:methods; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005821:Genetic Techniques / Q000295:instrumentation / Q000592:standards; D006801:Humans; D015336:Molecular Probe Techniques / Q000295:instrumentation / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods; D011786:Quality Control; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D017423:Sequence Analysis, RNA / Q000379:methods
chemical_list: D012333:RNA, Messenger; D012313:RNA
vernacular_title: Les méthodes d'extraction et de purification des ARN.