A new approach to oligonucleotide labelling using Diels-Alder cycloadditions and detection by SERRS.
Diels-Alder cycloaddition has been used to add a benzotriazole azo dye to a diene tagged oligonucleotide to generate unique SERRS signals at 10 attomoles.
D001391:Azo Compounds / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry*; D003500:Cyclization; D004247:DNA / Q000032:analysis; D005663:Furans / Q000737:chemistry; D008301:Maleimides / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties; D014230:Triazoles / Q000737:chemistry
D001391:Azo Compounds; D004396:Coloring Agents; D005663:Furans; D008301:Maleimides; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D014230:Triazoles; C043592:maleimide; C012771:benzotriazole; D004247:DNA