title: Two pathways of homologous recombination in Trypanosoma brucei.
abstract: African trypanosomes are unicellular parasites that use DNA recombination to evade the mammalian immune response. They do this in a process called antigenic variation, in which the parasites periodically switch the expression of VSG genes that encode distinct Variant Surface Glycoprotein coats. Recombination is used to move new VSG genes into specialised bloodstream VSG transcription sites. Genetic and molecular evidence has suggested that antigenic variation uses homologous recombination, but the detailed reaction pathways are not understood. In this study, we examine the recombination pathways used by trypanosomes to integrate transformed DNA into their genome, and show that they possess at least two pathways of homologous recombination. The primary mechanism is dependent upon RAD51, but a subsidiary pathway exists that is RAD51-independent. Both pathways contribute to antigenic variation. We show that the RAD51-independent pathway is capable of recombining DNA substrates with very short lengths of sequence homology and in some cases aberrant recombination reactions can be detected using such microhomologies.
mesh_terms: D000818:Animals; D000940:Antigenic Variation; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D051135:Rad51 Recombinase; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000254:growth & development; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan; D004268:DNA-Binding Proteins; D014643:Variant Surface Glycoproteins, Trypanosoma; D051135:Rad51 Recombinase