title: Determinant of the distribution volume at steady state for novel quinolone pazufloxacin in rats.
abstract: The distribution properties of the novel quinolone pazufloxacin (PZFX) in rats were compared with those of sparfloxacin (SPFX) and ofloxacin (OFLX). Following intravenous administration of these quinolonesto rats, the distribution volume atsteadystate (Vd(ss)) of PZFX wasfound to be 0.945 L kg(-1), whereas for OFLX and SPFX it was 1.83 and 3.42 L kg(-1), respectively. In order to understand this difference in Vd(ss), first the contribution of each tissue to the Vd(ss) using pharmacokinetic parameters was estimated and it was found that the type of tissue contributing the most was muscle. Subsequently, we determined the extent of tissue binding and uptake clearance (CLuptake) in the muscle. The ratio of unbound fraction of plasma to unbound fraction of muscle was almost equal for all quinolones tested, with values of 1.60, 1.52 and 1.32 for PZFX, OFLX and SPFX, respectively. In contrast, the tissue CLuptake of PZFX in the muscle (0.012 mL min(-1) g(-1)) was significantly lowerthan that of OFLX and SPFX (0.118 and 0.195 mL min(-1) g(-1), respectively). These results suggest that the low Vd(ss) value for PZFX can be attributed to the low CLuptake in the muscle.
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism / Q000493:pharmacokinetics*; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D024841:Fluoroquinolones; D008297:Male; D008657:Metabolic Clearance Rate; D009130:Muscle, Smooth / Q000378:metabolism; D015242:Ofloxacin / Q000378:metabolism / Q000493:pharmacokinetics; D010078:Oxazines / Q000378:metabolism / Q000493:pharmacokinetics*; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D000890:Anti-Infective Agents; D001798:Blood Proteins; D024841:Fluoroquinolones; D010078:Oxazines; C078052:pazufloxacin; D015242:Ofloxacin; C061363:sparfloxacin