Effects of 4-alkylmorpholine N-oxides on ATP-producing processes in Ehrlich ascites and L1210 leukaemia cells.
The main purpose of the present investigation was to study the effect of the homologous series of 4-alkylmorpholine N-oxides on ATP-producing processes in Ehrlich ascites and L1210 murine leukaemia cells. The effects on aerobic glucose consumption, lactic acid formation, content of total (T-SH) and non-protein thiol groups (NP-SH), endogenous and exogenous respiration and the level of ATP in tumour cells incubated in vitro were investigated. 4-Dodecylmorpholine N-oxide (DMNO), one of the most active compounds, decreased the level of ATP immediately after addition to the suspension of Ehrlich cells in an ice bath. After 2 h incubation at 37 degrees C the drop in the ATP level was much lower. A possible explanation for the decrease in the ATP level might be interaction of the amine oxide with the cell membrane.
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D003497:Cyclic N-Oxides / Q000138:chemical synthesis / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism; D007939:Leukemia L1210 / Q000378:metabolism*; D009025:Morpholines / Q000138:chemical synthesis / Q000494:pharmacology*; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010101:Oxygen Consumption / Q000187:drug effects; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D003497:Cyclic N-Oxides; D009025:Morpholines; D009363:Neoplasm Proteins; D013438:Sulfhydryl Compounds; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D005947:Glucose