title: Tenascin, E-cadherin and P2X calcium channel receptor expression is increased during rat blastocyst implantation.
abstract: The calcium-activated cell-adhesion proteins tenascin, E-cadherin and the purinergic (P2X) calcium channel receptors are expressed in an identical spatial and temporal pattern in uterine epithelium in the rat during implantation. On Day 1 of pregnancy (estrous), a diffuse cytoplasmic and specific basement membrane label for each of the proteins was observed throughout the uterine epithelium. On Day 3 of pregnancy, a specific and prominent lateral plasma membrane label for each protein was seen. At the time of implantation on Day 6, an additional and significant increase in the label for each was observed on the apical epithelium. At this time, the label for tenascin in the apical epithelium was increased 2.1-fold (p < 0.0004), that of E-cadherin was increased 2.5-fold (p < 0.0001) and the P2X receptor label was increased 2.0-fold (p < 0.0001). These observations suggest a major role for the calcium-activated adhesion proteins tenascin and E-cadherin in attachment and implantation, with ionic calcium for protein activation possibly provided by the P2X calcium channels. These events occur along the entire length of the uterine epithelium in preparation for blastocyst adhesion.
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism / Q000502:physiology*; D015820:Cadherins / Q000096:biosynthesis*; D015220:Calcium Channels / Q000096:biosynthesis*; D020013:Calcium Signaling / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D010064:Embryo Implantation / Q000502:physiology*; D004717:Endometrium / Q000166:cytology / Q000294:innervation / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D004971:Estrus / Q000502:physiology; D005260:Female; D007150:Immunohistochemistry; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis*; D058469:Receptors, Purinergic P2X; D019063:Tenascin / Q000096:biosynthesis*
chemical_list: D015820:Cadherins; D015220:Calcium Channels; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D019063:Tenascin