MagiProbe: a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator.
Fluorescence is the favored signaling technology for molecular diagnoses. Fluorescence energy transfer-based methods are powerful homogeneous assay tools. A novel oligonucleotide probe, named MagiProbe, which is simple to use, is described, and information given about the duplex formed with a target. The probe internally has a fluorophore and an intercalator. Its fluorescence is quenched by the intercalator in the absence of a target sequence. On hybridization with a target sequence, the probe emits marked fluorescence due to the interference in quenching by intercalation. Furthermore, MagiProbe hybridized with a single-base mismatched target emits less fluorescence than with a perfect matched target. It therefore can detect a single base difference in a double-stranded form with a target.
D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics; D065731:Cytochrome P-450 CYP2C19; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D015342:DNA Probes; D019793:Fluorescein / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D005914:Globins / Q000235:genetics; D006801:Humans; D007364:Intercalating Agents / Q000737:chemistry; D006899:Mixed Function Oxygenases / Q000235:genetics; D015394:Molecular Structure; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D011721:Pyrenes / Q000737:chemistry
D015342:DNA Probes; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D015345:Oligonucleotide Probes; D011721:Pyrenes; D005914:Globins; D004247:DNA; C030984:pyrene; D006899:Mixed Function Oxygenases; D001189:Aryl Hydrocarbon Hydroxylases; C045793:CYP2C19 protein, human; D065731:Cytochrome P-450 CYP2C19; D019793:Fluorescein