A transient receptor potential channel expressed in taste receptor cells.
We used differential screening of cDNAs from individual taste receptor cells to identify candidate taste transduction elements in mice. Among the differentially expressed clones, one encoded Trpm5, a member of the mammalian family of transient receptor potential (TRP) channels. We found Trpm5 to be expressed in a restricted manner, with particularly high levels in taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as alpha-gustducin, Ggamma13, phospholipase C-beta2 (PLC-beta2) and inositol 1,4,5-trisphosphate receptor type III (IP3R3). Our heterologous expression studies of Trpm5 indicate that it functions as a cationic channel that is gated when internal calcium stores are depleted. Trpm5 may be responsible for capacitative calcium entry in taste receptor cells that respond to bitter and/or sweet compounds.
D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D006224:Cricetinae; D015870:Gene Expression; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007527:Isoenzymes / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D009865:Oocytes / Q000502:physiology; D054799:Phospholipase C beta; D012333:RNA, Messenger / Q000032:analysis; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D050053:TRPM Cation Channels; D013649:Taste / Q000502:physiology*; D013650:Taste Buds / Q000502:physiology*; D015293:Transducin / Q000235:genetics; D010738:Type C Phospholipases / Q000378:metabolism; D014982:Xenopus laevis
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D050053:TRPM Cation Channels; C498617:Trpm5 protein, mouse; C075192:gustducin; D010738:Type C Phospholipases; D054799:Phospholipase C beta; C518037:Plcb2 protein, mouse; C518038:Plcb2 protein, rat; D015293:Transducin; D002118:Calcium