Scanning laser polarimetry in monkey eyes using variable corneal polarization compensation.
OBJECTIVE
To determine if scanning laser polarimetry (SLP) using variable anterior segment birefringence compensation can provide meaningful retinal nerve fiber layer (RNFL) thickness measurements in monkey eyes.


METHODS
A scanning laser polarimeter (GDx; Laser Diagnostic Technologies, San Diego, CA) was modified so that anterior segment birefringence could be compensated on an eye-specific basis. Six eyes of three adult Cynomolgus (Macaca fascicularis) monkeys were imaged. The authors determined the corneal polarization magnitude (CPM) and corneal polarization axis (CPA) in these eyes, and compared them with the fixed values in the commercial scanning laser polarimeter. Individually compensated RNFL images, using eye-specific CPM and CPA, were then obtained to determine if the resulting retardation profiles reflected the expected RNFL appearance observed with stereoscopic optic disc photographs. Two of the imaged monkeys had experimental glaucoma of the right eye, which allowed comparison of RNFL thickness measures between healthy eyes and those damaged by experimental glaucoma.


RESULTS
The CPM was small in each of the six eyes examined, ranging from 5.7 to 8.7 nm. The CPA ranged from -62 degrees to 78.7 degrees (nasally upward CPA values were recorded as negative; nasally downward CPA values were recorded as positive). These values are different from the values assumed by the commercially available fixed-compensator GDx. When eye-specific compensation was used, RNFL retardation profiles mimicked the expected appearance of the RNFL in all eyes. The authors also observed a substantial decrease in retardation in experimental glaucoma eyes compared with healthy fellow eyes.


CONCLUSIONS
Scanning laser polarimetry using eye-specific corneal polarization compensation can provide meaningful RNFL thickness measurements in monkey eyes. Observed differences in retardation between healthy and experimental glaucoma eyes suggest that SLP may be useful for detecting and monitoring RNFL loss in experimental primate glaucoma.
D000818:Animals; D001718:Birefringence; D003315:Cornea / Q000033:anatomy & histology*; D003941:Diagnostic Techniques, Ophthalmological / Q000295:instrumentation; D005901:Glaucoma / Q000175:diagnosis*; D007834:Lasers; D008252:Macaca fascicularis; D009412:Nerve Fibers / Q000473:pathology*; D009901:Optic Nerve Diseases / Q000175:diagnosis*; D012165:Retinal Ganglion Cells / Q000473:pathology*