Effect of platelet growth factors on proliferation of guinea pig bone marrow and splenic stromal precursor cells and proliferation of cultural descendants from bone marrow precursor cells.
Elimination of platelets from guinea pig splenocyte suspension (lacking megakaryocytes) with EDTA considerable reduces the efficiency cloning of splenic stromal precursor cells. It means that platelet-derived growth factors are necessary for stromal precursor cells from different organs (bone marrow, thymus, and spleen). The dependence on the platelet growth factors can vary within a wide range in descendants from cultured bone marrow precursor cells (passaged bone marrow fibroblasts at different stages of differentiation.
D000818:Animals; D001792:Blood Platelets / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D006133:Growth Substances / Q000494:pharmacology*; D006168:Guinea Pigs; D008297:Male; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects*
D006133:Growth Substances