[Study on point mutations in mitochondrial DNA control region for replication DLP(6) of cultured dermal fibroblast with 8-MOP/UVA treatment].
OBJECTIVE
To investigate the relationships between skin photoaging and point mutations in mitochondrial DNA (mtDNA) control region for replication of dermal fibroblast.


METHODS
Cultured dermal fibroblasts were treated by 8-methoxypsora len /ultraviolet A (8-MOP/UVA). mtDNA was extracted by one-step-method and th e PCR products of D-loop and adjacent transcription promoter (DLP(6)) fragment of mtDNA control region for replication were detected by polymerase chain reaction-single strain conformation polymorphism and direct sequencing.


RESULTS
After treated by 8-MOP/UVA, point mutations of 414 T-->G of DLP(6) fragment of mtDNA control region for replication largely accumulated.


CONCLUSIONS
Accumulation of point mutations of DLP(6) fragment of mtDNA control region for replication may be closely associated with skin photoaging.
D000328:Adult; D001665:Binding Sites; D002478:Cells, Cultured; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000187:drug effects / Q000528:radiation effects*; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D008297:Male; D008730:Methoxsalen / Q000494:pharmacology; D017319:Photosensitizing Agents / Q000494:pharmacology; D017354:Point Mutation; D014466:Ultraviolet Rays
D004272:DNA, Mitochondrial; D017319:Photosensitizing Agents; D008730:Methoxsalen