title: Endotoxins of enteric pathogens are chemotactic factors for human neutrophils.
abstract: Early activation of human peripheral blood polymorphonuclear neutrophils is characterized by their morphological changes from spherical to polarized shapes. The endotoxins from enteric pathogens (S. dysenteriae type 1, V. cholerae Inaba 569B, S. typhimurium, and K. pneumoniae) were assessed by their ability to induce morphological polarization of the neutrophils as measures of early activation. Phagocytic activity, adhesion, chemokinetic locomotion, and nitroblue tetrazolium (NBT) dye-reduction ability measured the later activation of the cells. Neutrophils showed distinct morphological polarization in suspension over a wide range of concentrations of these endotoxins when were compared with those that were induced by the standard chemotactic factor, N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP). It was discovered that all of the endotoxins induced locomotor responses in neutrophils in suspension that were dose- and time-dependent. The optimum concentration for the endotoxins of S. dysenteriae, V. cholerae, and K. pneumoniae was 1 mg/ml in which 71, 69, and 66% of the neutrophils were polarized. However, the S. typhimurium dose was 2 mg/ml in which 50% of the cells responded. Neutrophils that were stimulated with endotoxins also showed increased random locomotion (p<0.005) through cellulose nitrate filters, but an enhanced adhesion of the cells to glass surfaces (p<0.03). These are important functions of these cells to reach and phagocytose damaged cells, as well as invading microorganisms. Interestingly, the endotoxins had a highly-significant inhibitory effect upon the proportions of neutrophils phagocytosing opsonized yeast (p<0.01) with a small number of yeast that were engulfed by the cells (p<0.02). Further, endotoxin-treated cells showed an enhanced ability to reduce NBT dye (p<0.03). Therefore, we concluded that endotoxins of enteric pathogens are neutrophil chemotactic factors.
mesh_terms: D002448:Cell Adhesion; D002465:Cell Movement; D002630:Chemotactic Factors / Q000502:physiology*; D002633:Chemotaxis; D004731:Endotoxins / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007711:Klebsiella pneumoniae / Q000502:physiology*; D008070:Lipopolysaccharides; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology; D010587:Phagocytosis; D012762:Shigella dysenteriae / Q000502:physiology*; D013997:Time Factors; D014734:Vibrio cholerae / Q000502:physiology*
chemical_list: D002630:Chemotactic Factors; D004731:Endotoxins; D008070:Lipopolysaccharides