title: Flanking sequence tags in Arabidopsis thaliana T-DNA insertion lines: a pilot study.
abstract: Eight hundred and fifty Arabidopsis thaliana T-DNA insertion lines have been selected on a phenotypic basis. The T-DNA flanking sequences (FST) have been isolated using a PCR amplification procedure and sequenced. Seven hundred plant DNA sequences have been obtained revealing a T-DNA insertion in, or in the immediate vicinity of 482 annotated genes. Limited deletions of plant DNA have been observed at the site of insertion of T-DNA as well as in its left (LB) and right (RB) T-DNA signal sequences. The distribution of the T-DNA insertions along the chromosomes shows that they are essentially absent from the centrometric and pericentrometric regions.
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D010865:Pilot Projects; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D018744:DNA, Plant; D004277:DNA, Single-Stranded; C036483:T-DNA