Iodoinsulin used to determine specific activity of iodine-131.
Anodal mobility of iodoinsulin on starch-gel electrophoresis increases progressively as the number of iodine atoms substituted in the molecule increases. The iodine content of iodine-131 solutions is determined by comparison of autoradiographic patterns of insulin heavily labeled with iodine-131 and of insulins lightly labeled with iodine-131 and known quantities of iodine-127.
D001345:Autoradiography; D004592:Electrophoresis, Starch Gel; D007328:Insulin / Q000031:analogs & derivatives* / Q000032:analysis; D007455:Iodine / Q000032:analysis; D007457:Iodine Radioisotopes / Q000032:analysis*; D012015:Reference Standards
D007328:Insulin; D007457:Iodine Radioisotopes; C008147:insulin, iodo-; D007455:Iodine