Coating with genetic engineered hydrophobin promotes growth of fibroblasts on a hydrophobic solid.
Class I Hydrophobins self-assemble at hydrophilic-hydrophobic interfaces into a highly insoluble amphipathic film. Upon self-assembly of these fungal proteins hydrophobic solids turn hydrophilic, while hydrophilic materials can be made hydrophobic. Hydrophobins thus change the nature of a surface. This property makes them interesting candidates to improve physio- and physico-chemical properties of implant surfaces. We here show that growth of fibroblasts on Teflon can be improved by coating the solid with genetically engineered SC3 hydrophobin. Either deleting a stretch of 25 amino acids at the N-terminus of the mature hydrophobin (TrSC3) or fusing the RGD peptide to this end (RGD-SC3) improved growth of fibroblasts on the solid surface. In addition, we have shown that assembled SC3 and TrSC3 are not toxic when added to the medium of a cell culture of fibroblasts in amounts up to 125 microg ml(-1).
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004396:Coloring Agents / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000494:pharmacology*; D017353:Gene Deletion; D005818:Genetic Engineering; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D012567:Schizophyllum / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors
D020099:Coated Materials, Biocompatible; D004396:Coloring Agents; D005656:Fungal Proteins; D010455:Peptides; D013778:Tetrazolium Salts; D013844:Thiazoles; D011138:Polytetrafluoroethylene; C022616:thiazolyl blue