title: Procyanidins from Vitis vinifera seeds: in vivo effects on oxidative stress.
abstract: The purpose of this study was to evaluate the effect of supplementation with procyanidins from Vitis vinifera on markers of oxidative stress. Ten healthy volunteers received a daily dose of 110 mg of procyanidins for 30 days. Fasting venous blood samples were taken before and at the end of the supplementation period and after 7 days of wash-out. The total antioxidant activity and the plasma concentrations of alpha-tocopherol were not modified. Conversely, the levels of alpha-tocopherol in red blood cell membranes increased significantly from 1.8 +/- 0.1 to 2.8 +/- 0.2 mg/g. Similarly, the lymphocyte oxidized DNA [8-oxo-7,8-dihydro-2'-deoxyguanosine/2'-deoxyguanosine ratio] was reduced from 7.23 +/- 2.47 to 2.34 +/- 0.51, and the red blood cell membrane fatty acid composition shifted to a higher level of polyunsaturated fatty acids. On the basis of these results, it may be suggested that dietary procyanidins exert their antioxidant protection in vivo by sparing liposoluble vitamin E and reducing DNA oxidative damage.
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage*; D044946:Biflavonoids; D002392:Catechin / Q000008:administration & dosage* / Q000032:analysis; D004249:DNA Damage; D004032:Diet; D019587:Dietary Supplements; D004910:Erythrocyte Membrane / Q000737:chemistry; D005227:Fatty Acids / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000097:blood; D006801:Humans; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000008:administration & dosage; D010936:Plant Extracts / Q000737:chemistry; D044945:Proanthocyanidins; D012639:Seeds / Q000737:chemistry*; D011795:Surveys and Questionnaires; D027843:Vitis / Q000737:chemistry*; D024502:alpha-Tocopherol / Q000097:blood
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010636:Phenols; D010936:Plant Extracts; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin; D024502:alpha-Tocopherol