6-Sulfo LacNAc, a novel carbohydrate modification of PSGL-1, defines an inflammatory type of human dendritic cells.
The monoclonal antibody M-DC8 defines a major subset of human blood dendritic cells (DCs). Here we identify the M-DC8 structure as 6-sulfo LacNAc, a novel carbohydrate modification of the P selectin glycoprotein ligand 1 (PSGL-1). In contrast to previously described blood DCs, M-DC8+ DCs lack the cutaneous lymphocyte antigen (CLA) on PSGL-1 and fail to bind P and E selectin. Yet they express anaphylatoxin receptors (C5aR and C3aR) and the Fcgamma receptor III (CD16), which recruit cells to inflammatory sites. While sharing with DC1 the expression of myeloid markers and a potent capacity to prime T cells in vitro, M-DC8+ DCs produce far more TNF-alpha in response to the bacterial endotoxin lipopolysaccharide (LPS). Thus, 6-sulfo LacNAc-expressing DCs appear as a novel proinflammatory DC subset.
D000328:Adult; D000606:Amino Sugars; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis; D001773:Blood Cells / Q000276:immunology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002465:Cell Movement; D003713:Dendritic Cells / Q000145:classification / Q000276:immunology* / Q000637:transplantation; D019040:E-Selectin / Q000378:metabolism; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D006031:Glycosylation; D006801:Humans; D007249:Inflammation / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology* / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000276:immunology; D008565:Membrane Proteins; D051379:Mice; D016513:Mice, SCID; D008969:Molecular Sequence Data; D019007:P-Selectin / Q000378:metabolism; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement / Q000032:analysis; D017452:Receptors, IgG / Q000032:analysis; D014183:Transplantation, Heterologous; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
C467438:6-sulfo-LacNac; D000606:Amino Sugars; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D019040:E-Selectin; D000939:Epitopes; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D019007:P-Selectin; C084882:P-selectin ligand protein; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha; C085567:complement C3a receptor