title: Murine calmodulin binding protein 1 (Calmbp1): tissue-specific expression during development and in adult tissues.
abstract: Expression of the 1.9 kb cDNA of murine Calmbp1 has been shown to interfere with the mitotic S-M checkpoint in yeast (J. Cell Sci. 111 (1998) 3609). The physiological function and expression pattern of Calmbp1 in mice, however, are unknown. We have investigated the expression of Calmbp1 in mid-gestation and late-gestation fetuses and in adult organs of the mouse. In Northern blot analyses, using a Calmbp1-specific probe, a single mRNA of more than 7.4 kb was found that showed a progressive decline in total RNA preparations of fetal heads during the period from day E12 to E16. In the adult, this Calmbp1 transcript was detectable by Northern blot analysis exclusively in testis, ovary and spleen of all organs examined. In situ hybridizations revealed that Calmbp1 is expressed (a) in the differentiating central and peripheral nervous system, (b) in the epithelial cells lining the crypts of the small intestine in late gestation and adult mice, (c) in the fetal, but not the adult liver, (d) in both the fetal and adult spleen, where the signal colocalized with hematopoetic cells in the red pulp, (e) in late gestation embryos in the thymus, S-shaped tubules in the kidney, epidermis, and (f) in leptotene, zygotene and pachytene spermatocytes of the adult testis and the follicle epithelium of the activated follicles in the adult ovary.
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002148:Calmodulin-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D018076:DNA, Complementary / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins; D010053:Ovary / Q000196:embryology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013091:Spermatogenesis; D013737:Testis / Q000196:embryology / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: C512460:ASPM protein, mouse; D002148:Calmodulin-Binding Proteins; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger