Reduction of contamination of mycobacterial growth indicator tubes with a modified antimicrobial combination.
Culture in the fluorimetric Mycobacteria Growth Indicator Tube (MGIT) treated with a combination of vancomycin, amphotericin B, and nalidixic acid (VAN) showed growth of most strains of 31 mycobacterial species with a less-than-1-day delay. The results were similar to those in the MGIT with polymyxin B, amphotericin B, nalidixic acid, trimethoprim, and azlocillin, but with respiratory specimens, the MGIT with VAN showed a lower contamination rate with no change in the detection rate or time.
D000666:Amphotericin B / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001390:Azlocillin / Q000494:pharmacology; D004347:Drug Interactions; D006801:Humans; D009161:Mycobacterium / Q000187:drug effects* / Q000254:growth & development; D009268:Nalidixic Acid / Q000494:pharmacology; D011112:Polymyxin B / Q000494:pharmacology; D014295:Trimethoprim / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D009268:Nalidixic Acid; D014640:Vancomycin; D000666:Amphotericin B; D014295:Trimethoprim; D001390:Azlocillin; D011112:Polymyxin B