Differential regulation of thymus- and activation-regulated chemokine induced by IL-4, IL-13, TNF-alpha and IFN-gamma in human keratinocyte and fibroblast.
The CC chemokine thymus- and activation-regulated chemokine (TARC/CCL17) acts on CC chemokine receptor 4 (CCR4), which is known to be selectively expressed in Th2 cells. In order to compare the regulatory profiles of TARC production by tumor necrosis factor-alpha (TNF-alpha), IFN-gamma, interleukin-4 (IL-4) and IL-13 in keratinocytes and fibroblasts, HaCaT cells, a human keratinocyte cell line, and NG1RGB cells, a human skin fibroblast cell line, were used. The expression of TARC protein was measured using enzyme-linked immunosorbent assay (ELISA), and the mRNA level was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The spontaneous expression of TARC protein and mRNA levels were augmented by TNF-alpha and IFN-gamma and were inhibited by IL-4 and IL-13 in the keratinocytes. The fibroblasts expressed the TARC protein and mRNA only in the presence of IL-4+TNF-alpha or IL-13+TNF-alpha stimulation. IFN-gamma further enhanced the IL-4+TNF-alpha or IL-13+TNF-alpha-induced TARC production in the fibroblasts. Thus, TNF-alpha and IFN-gamma -induced TARC production was differentially regulated by IL-4 and IL-13 in human keratinocytes and fibroblasts.
D001483:Base Sequence; D002460:Cell Line; D054414:Chemokine CCL17; D018925:Chemokines / Q000235:genetics*; D019742:Chemokines, CC / Q000235:genetics*; D017931:DNA Primers; D005347:Fibroblasts / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D018793:Interleukin-13 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D015603:Keratinocytes / Q000276:immunology*; D007700:Kinetics; D008213:Lymphocyte Activation; D012333:RNA, Messenger / Q000235:genetics; D012867:Skin / Q000166:cytology / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
C106230:CCL17 protein, human; D054414:Chemokine CCL17; D018925:Chemokines; D019742:Chemokines, CC; D017931:DNA Primers; D018793:Interleukin-13; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007371:Interferon-gamma