Expression of a green fluorescence protein-carrier protein into mouse spermatozoa.
Intra-testicular inoculation of an adenoviral vector carrying the fusion gene Aequorea victoria green fluorescence protein/rat-liver glycogen synthase (GFP/LGS) resulted in the presence of GFP/GLS in spermatozoa from 7days to, at least, 16days after inoculation. The GFP/LGS was detected in the sperm heads after an "in vitro" fertilization procedure, either before or after the oocyte penetration. Our results indicate that spermatozoa carrying GFP/LGS protein conserved their fertilizing ability and were also detectable after oocyte penetration. This technique will allow to develop an easy system to follow the fate of mature sperm proteins.
D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D017930:Genes, Reporter; D005822:Genetic Vectors; D006006:Glycogen Synthase / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D037401:Scyphozoa; D013094:Spermatozoa / Q000378:metabolism*; D013997:Time Factors
D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D006006:Glycogen Synthase