The proteomics of senescence in leaves of white clover, Trifolium repens (L.).
A proteomics approach has been used to study changes in protein abundance during leaf senescence in white clover. Changes in cell ultrastructure were also examined using transmission electron microscopy. The most obvious ultrastructural changes during senescence occurred in chloroplasts, with progressive loss of thylakoid integrity and accumulation of osmiophilic globules in the stroma. Quantitative analysis of 590 leaf protein spots separated by two-dimensional electrophoresis indicated that approximately 40% of the spots showed significant senescence related changes in abundance. Approximately one-third of the protein spots present in mature green leaves were also visible by two-dimensional electrophoresis of an isolated chloroplast fraction, and these spots represented a major proportion of the proteins showing senescence related declines in abundance. Chloroplast proteins that were identified by matrix-assisted laser desorption/ionization-time of flight mass fingerprinting included rubisco large and small subunits, a rubisco activase and the 33 kDa protein of the photosystem II oxygen-evolving complex. These proteins declined in abundance late in senescence, indicating that the photosynthetic apparatus was being degraded. A chloroplast glutamine synthetase showed partial decline in abundance during late senescence but was maintained at levels that may support provision of glutamine for export to other tissues. The results emphasise the importance of proteolysis, chloroplast degradation and remobilisation of nitrogen in leaf senescence.
D016922:Cellular Senescence; D002734:Chlorophyll / Q000737:chemistry* / Q000378:metabolism; D002736:Chloroplasts / Q000648:ultrastructure*; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000378:metabolism* / Q000648:ultrastructure; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D029921:Trifolium / Q000737:chemistry* / Q000378:metabolism; D015854:Up-Regulation
D020543:Proteome; D002734:Chlorophyll; D005974:Glutamate-Ammonia Ligase