title: Molecular cloning and sequencing of partial cDNA of tumor necrosis factor and p75 tumor necrosis factor receptor of Syrian golden hamster (Mesocricetus auratus) with the use of universal primers.
abstract: In this study we cloned and analysed partial cDNA of tumor necrosis factor (TNF) and p75 TNF-R receptor of Syrian golden hamster (Mesocricetus auratus). We obtained a 382-bp sequence of TNF and a 148-bp sequence coding for p75 TNF-R. The primers used for the cloning were designed on the basis of inter-species homology, thus presumably can be used for cloning and analysis of TNF and p75 TNF-R genes of other mammals.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D008297:Male; D008647:Mesocricetus / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha