Evaluation of adriamycin induced DNA damage and repair in human and animal cells.
Adriamycin induced DNA damage and repair were investigated by the comet assay in four human cell lines (CRL2088, ME18, Lu106, WISH) and five animal cell lines (L929, Balb/3T3, CHO. MDBK, Vero). The results indicated the concentration-dependent induction of DNA strand breaks in all cell lines after adriamycin treatment. Simultaneously, wide differences in sensitivity of cells to the damaging action of adriamycin were observed. The most sensitive were two human cell lines derived from epithelial tissues, Lu106 and WISH. In these lines the breaks induced by adriamycin were not repaired within 6 h postincubation. In two other human cell lines, CRL2088 and ME18, only a small number of DNA breaks appeared after adriamycin exposure and these were very quickly repaired. Among the animal cell lines only the mouse cell line L929 was very sensitive to adriamycin, but most of the induced breaks were repaired within 6 h postincubation. The remaining animal cell lines were less sensitive to DNA damage by adriamycin and all except 3T3, completely repaired the breaks within 6 h postincubation.
D000818:Animals; D016466:CHO Cells; D002417:Cattle; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004249:DNA Damage / Q000187:drug effects* / Q000502:physiology; D004260:DNA Repair / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D004341:Drug Evaluation / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D014709:Vero Cells
D004317:Doxorubicin