title: The high-speed Hydra-Plus-One system for automated high-throughput protein crystallography.
abstract: An automated high-throughput dispenser has been developed for the setup of protein crystallization trials by vapor diffusion or Microbatch methods. The Hydra-Plus-One is composed of a Hydra-PP system equipped with a motorized XYZ-platform, 96 precision glass syringes and a single-channel microsolenoid dispenser, which transfers 100 nl-50 micro l of protein solution with an accuracy of > 90% at a speed of 60s per 96 wells. Up to 300 micro l of premixed cocktails can be aspirated with the 96-syringe-assembly and dispensed into reservoir and droplet wells within 60s. The Hydra-Plus-One combines high precision, reliability and speed in a cost-effective high-throughput system ideally suited for protein crystallization
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D003461:Crystallography / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D009169:Mycobacterium tuberculosis / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D001426:Bacterial Proteins; D011506:Proteins