title: Sucrose stimulates branching morphogenesis of embryonic mouse lung in vitro: a problem of osmotic balance between lumen fluid and culture medium.
abstract: In organ cultures of lung rudiments from 11-day mouse embryos, it was found that addition of sucrose to the culture medium stimulated branching morphogenesis and reduced lumen distension. Two possible roles of sucrose were postulated: one as a nutrient and another as a generator of osmotic pressure inducing osmosis of water from the lumen fluid to the culture medium across a simple columnar epithelial cell layer. To assess which was the case, branching morphogenesis was investigated in lung rudiments cultured in medium in which osmotic pressure was increased by the addition of lactose or NaCl rather than sucrose: similar acceleration of branching was observed in both. In another experiment, lumen fluid of cultured lung rudiments was mechanically drained each day, and significantly stimulated branching morphogenesis was observed even when sucrose was not added to the culture medium. Heparin is known to induce abnormal lumen distension and inhibits branching morphogenesis. Heparin-induced abnormal morphogenesis was prevented either by the addition of sucrose to the culture medium or by the mechanical drainage of lumen fluid. These results suggest that lumen distension caused by the accumulation of lumen fluid disrupts lung branching morphogenesis in vitro, even when the mechanism of branching morphogenesis is intact.
authors.affiliation: Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Inage-ku, Chiba 263-8522, Japan. nogawa@bio.s.chiba-u.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nogawa
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors / Q000378:metabolism; D066298:In Vitro Techniques; D008168:Lung / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice / Q000196:embryology*; D009997:Osmotic Pressure / Q000187:drug effects*; D013395:Sucrose / Q000494:pharmacology*
chemical_list: C496454:Fgf7 protein, mouse; D051523:Fibroblast Growth Factor 7; D013395:Sucrose; D005346:Fibroblast Growth Factors