Influence of media nutrients on synthesis of lignin peroxidase from Aspergillus sp.
The effect of carbon and nitrogen sources, lignocellulosic substrates, and metal ions on lignin peroxidase (LiP) activity of Aspergillus sp., which was isolated from a mangrove area, was studied. Glucose (1%) was found to be the best carbon source. Among the various lignocellulosic substrates used, coir pith at 3% concentration increased LiP activity twofold on the second day of incubation. Peptone and KNO3 completely inhibited the enzyme synthesis while (NH4)2SO4 at 12.5 mM produced maximum activity. Since seawater contained all the requisite metal ions, any added ions had a negative effect on activity. Cu2+ had the most inhibiting effect while K+ the least. When all the optimized conditions were provided, in nitrogen- and carbon-sufficient medium, a maximum LiP activity of 345 U/mL was obtained on the second day of incubation.
Ahammed
Shamla
S
Biochemical Processing Division, Regional Research Laboratory, Trivandrum, India.
Prema
Parukuttyamma
P
D000641:Ammonia / Q000378:metabolism; D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development; D002482:Cellulose / Q000378:metabolism; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D007477:Ions; D008031:Lignin / Q000378:metabolism; D008670:Metals / Q000737:chemistry; D010544:Peroxidases / Q000096:biosynthesis* / Q000378:metabolism
D003470:Culture Media; D007477:Ions; D008670:Metals; C036909:lignocellulose; D000641:Ammonia; D002482:Cellulose; D008031:Lignin; D010544:Peroxidases; C042858:lignin peroxidase; D005947:Glucose