title: GENOTRACE: cDNA-based local GENOme assembly from TRACE archives.
abstract: GENOTRACE identifies the genomic organization for a cDNA using raw data from genome sequencing projects in progress (trace archives). Local genomic contigs are generated, allowing for example the design of PCR primers in intronic sequences to amplify coding regions of a gene, needed for example for mutation or SNP detection.
  
  
  BACKGROUND
  The package and examples of output files can be downloaded from http://rat.niob.knaw.nl/GENOTRACE
authors.affiliation: Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
authors.forename: Eugene
authors.initials: E
authors.lastname: Berezikov
authors.forename: Ronald H A
authors.initials: RH
authors.lastname: Plasterk
authors.forename: Edwin
authors.initials: E
authors.lastname: Cuppen
mesh_terms: D001109:Archives; D002874:Chromosome Mapping / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics*; D003628:Database Management Systems; D030561:Databases, Nucleic Acid; D019146:Hypermedia; D016247:Information Storage and Retrieval / Q000379:methods*; D020407:Internet; D009317:National Library of Medicine (U.S.); D017422:Sequence Analysis, DNA / Q000379:methods; D012984:Software; D012985:Software Design; D014481:United States
chemical_list: D018076:DNA, Complementary