Potential role of Homer-2a on cutaneous vascular anomaly.
Homer protein was identified based on its rapid induction in rat hippocampal granule cell neurons following excitatory synaptic activity. Although the presence of the Homer gene in the peripheral tissues has been observed in previous reports, the physiological function of the Homer protein in these tissues has not been noted. In this experiment, a Homer-2a cDNA fragment was successfully amplified by RTPCR in the involuting phase of human hemangioma but not in the human vascular malformation and normal vessel. After isolation of full Homer cDNA in a mouse liver cDNA library, E1-deleted recombinant adenovirus expressing the Homer protein (Adv.CMV.mHomer-2a) was constructed to determine its physiological function in peripheral tissues. Adv.CMV.mHomer2a, but not Adv.CMV.LacZ (recombinant adenovirus expressing Beta-galactosidase), strongly inhibited the growth rate of HUVECs (human umbilical vein endothelial cells) probably via inducing apoptosis determined by acridine orange/ethidium bromide (AO/EB) staining methods. This study suggests that the Homer gene is present in human specimens in the involuting phase of hemangioma, and it might be involved in the growth control.
Kim
Jeong Tae
JT
Department of Plastic and Reconstructive Surgery, Dong-A University, Busan, Korea.
Park
Si Hyun
SH
Kim
Soek Kwun
SK
Kwon
Eun-Young
EY
Do
Mi-Hyang
MH
Hwang
Tae Ho
TH
D000293:Adolescent; D000328:Adult; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D001808:Blood Vessels / Q000002:abnormalities*; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D018076:DNA, Complementary / Q000235:genetics; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D006391:Hemangioma / Q000098:blood supply / Q000235:genetics*; D000072233:Homer Scaffolding Proteins; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009479:Neuropeptides / Q000235:genetics / Q000502:physiology*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000098:blood supply; D012878:Skin Neoplasms / Q000098:blood supply / Q000235:genetics*
D002352:Carrier Proteins; D018076:DNA, Complementary; D000072233:Homer Scaffolding Proteins; D009479:Neuropeptides