title: Analysis of surface properties of human cancer cells using derivatized beads.
abstract: Standard histochemical analysis of cells and tissues generally involves procedures that utilize a relatively small number of probes such as dyes, and generally requires hours or days to process. Our laboratory has developed a novel method for histochemical surveys of cell surface properties that utilizes a large number of probes (derivatized agarose beads) and takes seconds or minutes to accomplish. In this study, 4 human cell lines (CCL-255 (LS123) human colon cancer cells that are non-tumorigenic in nude mice; CRL-1459 (CCD-18CO) human colon endothelial cells that are non-malignant; CCL-220 (COLO 320DM) human colon cancer cells that are tumorigenic in nude mice; and HTB-171 (NCI H446) human lung carcinoma cells) were tested for their ability to bind to agarose beads derivatized with 51 different molecules. There were statistically significant differences in binding of the 4 cell types to all of the 51 types of beads, but 15 types of beads showed dramatic differences in binding to one or more of the 4 cell types. For example, only HTB-171 (NCI H446) bound to p-aminophenyl-beta-D-glucopyranoside-derivatized beads and only CCL-220 (COLO 320DM) bound to L-tyrosine-derivatized beads. The specificity of cell-bead binding was examined by performing assays in the presence or absence of exogenously added compounds in hapten-type of inhibition experiments. This assay, that utilizes large numbers of novel probes, may help in the development of new libraries of surface properties of specific cell types, with differing degrees of malignancy, that at this time could not be developed by using other available technologies.
authors.affiliation: Department of Biology and Center for Cancer and Developmental Biology, California State University, Northridge 91330-8303, USA.
authors.forename: Maria R
authors.initials: MR
authors.lastname: Khurrum
authors.forename: Gayani R
authors.initials: GR
authors.lastname: Weerasinghe
authors.forename: Evelyn S
authors.initials: ES
authors.lastname: Soriano
authors.forename: Rashad
authors.initials: R
authors.lastname: Riman
authors.forename: Oliver
authors.initials: O
authors.lastname: Badali
authors.forename: Stephanie
authors.initials: S
authors.lastname: Gipson
authors.forename: Jessica
authors.initials: J
authors.lastname: Medina
authors.forename: Juan
authors.initials: J
authors.lastname: Alfaro
authors.forename: Vanessa M
authors.initials: VM
authors.lastname: Navarro
authors.forename: Caroline B
authors.initials: CB
authors.lastname: Harieg
authors.forename: Lylla
authors.initials: L
authors.lastname: Ngo
authors.forename: Tharinee
authors.initials: T
authors.lastname: Sakhakorn
authors.forename: Lital
authors.initials: L
authors.lastname: Kirszenbaum
authors.forename: David
authors.initials: D
authors.lastname: Khatibi
authors.forename: Karolin
authors.initials: K
authors.lastname: Abedi
authors.forename: Marcela
authors.initials: M
authors.lastname: Barajas
authors.forename: Gregory C
authors.initials: GC
authors.lastname: Zem
authors.forename: Adit
authors.initials: A
authors.lastname: Kirszenbaum
authors.forename: Arash
authors.initials: A
authors.lastname: Razi
authors.forename: Steven B
authors.initials: SB
authors.lastname: Oppenheimer
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D054418:Chemokine CCL20; D019742:Chemokines, CC / Q000737:chemistry / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D006639:Histidine / Q000737:chemistry; D006651:Histocytochemistry; D006801:Humans; D019402:Macrophage Inflammatory Proteins / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008863:Microspheres; D054399:Receptors, CCR6; D019707:Receptors, Chemokine; D012685:Sepharose / Q000737:chemistry; D014407:Tumor Cells, Cultured
chemical_list: C108355:CCL20 protein, human; C106311:CCR6 protein, human; D054418:Chemokine CCL20; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D054399:Receptors, CCR6; D019707:Receptors, Chemokine; D003208:Concanavalin A; D006639:Histidine; D012685:Sepharose; D001120:Arginine