Preparation and identification of monoclonal antibodies against human brain-derived neurotrophic factor.
OBJECTIVE
To prepare monoclonal antibodies (mAbs) against human brain-derived neurotrophic factor (BDNF).


METHODS
BALB/C mice were immunized with purified BDNF protein in conjunction with acid-treated Salmonella (antigen:thallus=1:5), and mAbs were subsequently derived by hybridoma technique.


RESULTS
Three hybridoma cell lines secreting anti-BDNF mAbs were obtained, designated as B1, B2 and 4D1 respectively and all categorized into IgG1 subtype. The titers of the mAbs in the ascitic fluid of the rats ranged from 1x10(6) to 1x10(5), and their relative affinities were B2>B1>4D1.


CONCLUSIONS
mAbs against BDNF are successfully prepared, which can be instrumental for further study of the expression and distribution of BDNF in vivo, and the detection of BDNF produced by genetic engineering.
Su
Jin
J
Department of Respiratory Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Zhang
Ya-Li
YL
You
Chang-Xuan
CX
Wu
Jin-Ya
JY
Che
Xiao-Yan
XY
Wang
Xiao-Ning
XN
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001921:Brain; D019208:Brain-Derived Neurotrophic Factor / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D019208:Brain-Derived Neurotrophic Factor