[Expression analysis of protein tyrosine kinases of the FAK (focal adhesion kinase) family in osteosarcoma].
OBJECTIVE
Expression analysis of the protein tyrosine kinases, focal adhesion kinase (FAK) and proline-rich tyrosine kinase2 (Pyk2) in high grade osteosarcomas.


METHODS
Expression of the kinases was evaluated qualitatively by immunohistochemical staining and quantitatively by real-time PCR.


RESULTS
Osteoblastic cells of high grade osteosarcomas show a distinct FAK expression but an overexpression at the transcriptional level could not be detected. The Pyk2-mRNA expression was decreased in osteosarcomas.


CONCLUSIONS
An altered relationship of FAK and Pyk2 was observed for different tumors and could also be important for osteosarcoma development.
Schröder
A
A
Abteilung Osteopathologie/Zentrum für Biomechanik, Pathologisches Institut, Universitätskrankenhaus Hamburg-Eppendorf, Hamburg, Germany.
Delling
G
G
Kaiser
E A
EA
D000293:Adolescent; D000328:Adult; D000368:Aged; D001859:Bone Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D011505:Protein-Tyrosine Kinases / Q000235:genetics*
D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496050:PTK2 protein, human