title: Investigation of cellular metallic elements in single neurons of human brain tissues.
abstract: The purpose of this report is to introduce the potential of synchrotron radiation X-ray fluorescence (SRXRF) spectroscopy, by describing the application of this method to mapping and quantification of metallic elements in neurons from brain tissues affected by Alzheimer's disease (AD). In this study, clear images of Fe, Zn and Ca within certain single neurons were obtained. There was a high degree of correlation between Zn and Ca in the groups of cells with lower and relatively higher levels of Ca. These results demonstrate that further investigation with larger numbers of neurons using SRXRF spectroscopy may contribute to advancing the knowledge of the mechanisms of metal-induced cell degeneration in human brain tissues, including those affected by AD.
authors.affiliation: Department of Psycgiatry, Nagoya University Graduate School of Medicine, Japan. ishiyo@med.nagoya-u.ac.jp
authors.forename: Ryoko
authors.initials: R
authors.lastname: Ishihara
authors.forename: Ari
authors.initials: A
authors.lastname: Ide-Ektessabi
authors.forename: Kenji
authors.initials: K
authors.lastname: Ikeda
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Mizuno
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Fujisawa
authors.forename: Tohru
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Ohta
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D001921:Brain / Q000378:metabolism* / Q000503:physiopathology; D002118:Calcium / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000378:metabolism; D008670:Metals / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D013052:Spectrometry, X-Ray Emission / Q000379:methods*; D017356:Synchrotrons; D015032:Zinc / Q000378:metabolism
chemical_list: D008670:Metals; D007501:Iron; D015032:Zinc; D002118:Calcium