title: Comparison of tracheal aspirate and bronchoalveolar lavage specimens from premature infants.
abstract: Lung fluid obtained by tracheal aspiration (TA) or bronchoalveolar lavage (BAL) has been used to study bronchopulmonary dysplasia (BPD). These two sample collection methods have seldom been compared. Paired BAL and TA specimens were collected 1, 3, 7 and 28 days after birth in 40 infants <34 weeks' gestation during a randomized, controlled trial of dexamethasone for BPD prophylaxis. Interleukin 8 (IL-8) and cell counts were measured. Compared to subjects without BPD, those who developed BPD or died by 28 days had elevated IL-8 in epithelial lining fluid on day 1 in both BAL specimens (20 ng/ml vs. 2 ng/ml) and TA specimens (101 ng/ml vs. 18 ng/ml). IL-8 levels (r = 0.55) and neutrophil proportions (r = 0.51) were moderately correlated between BAL and TA samples. TA specimens may be suitable substitutes for BAL samples in some studies of newborn lung fluid.
authors.affiliation: Department of Pediatrics (Neonatology), Strong Children's Research Center, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA. carl_dangio@urmc.rochester.edu
authors.forename: Carl T
authors.initials: CT
authors.lastname: D'Angio
authors.forename: Kumar
authors.initials: K
authors.lastname: Basavegowda
authors.forename: Nelly E
authors.initials: NE
authors.lastname: Avissar
authors.forename: Jacob N
authors.initials: JN
authors.lastname: Finkelstein
authors.forename: Robert A
authors.initials: RA
authors.lastname: Sinkin
mesh_terms: D001826:Body Fluids / Q000737:chemistry*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D001997:Bronchopulmonary Dysplasia / Q000175:diagnosis* / Q000517:prevention & control; D003907:Dexamethasone / Q000627:therapeutic use; D004311:Double-Blind Method; D005865:Gestational Age; D005938:Glucocorticoids / Q000627:therapeutic use; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D016209:Interleukin-8 / Q000032:analysis*; D007958:Leukocyte Count; D009504:Neutrophils; D010919:Placebos; D011379:Prognosis; D012680:Sensitivity and Specificity; D013396:Suction; D014132:Trachea; D014508:Urea / Q000032:analysis / Q000097:blood
chemical_list: D005938:Glucocorticoids; D016209:Interleukin-8; D010919:Placebos; D003907:Dexamethasone; D014508:Urea