Neonatal ovine adrenal cortical and medullary Cell H+ responses to ACTH and prostaglandin E(2).
The microphysiometric technique was used to evaluate 1- and 3-day-old lamb adrenal gland H+ production in response to ACTH or PGE(2). ACTH stimulated cortical, but not medullary H+ production; maximal H+ by combined cortical and medullary cells was greater than by cortical cells alone (p < 0.05). In contrast, the magnitude of the H+ response to PGE(2) was greater with medullary than with cortical cells alone or with cortical and medullary cells combined (p < 0.05). The H+ response to ACTH was greater in 3-day-old compared to 1-day-old lambs while the potency of PGE(2) was not different at the ages studied. We conclude that in neonatal sheep: (1) ACTH and PGE(2) are potentially important in adrenal regulation, and (2) paracrine communication appears to be functioning as has been shown for adults.
Zambrano
E
E
Laboratory for Pregnancy and Newborn Research, College of Veterinary Medicine, Department of Biomedical Sciences, Cornell University, Ithaca, N.Y. 14853, USA.
Nathanielsz
P W
PW
McDonald
T J
TJ
D000302:Adrenal Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000313:Adrenal Medulla / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D015232:Dinoprostone / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011522:Protons; D012756:Sheep
D011522:Protons; D000324:Adrenocorticotropic Hormone; D015232:Dinoprostone