title: Vesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts.
abstract: A salivary gland extract (SGE) prepared from 5-days-fed Dermacentor reticulatus female ticks was fractionated by fast protein liquid chromatography (FPLC). The effect of three FPLC fractions selected on the basis of anti-interleukin 8 (anti-IL-8) activity on vesicular stomatitis virus (VSV) nucleocapsid (N) protein formation in mouse L-cells was determined. Infected 14C-labeled cells treated with the FPLC fractions were analyzed by two-dimensional (2D) electrophoresis. The yields of VSV N protein were evaluated by Imagemaster software analysis. Most noticeable was an increase in the N protein production after treatment with the fraction 39 corresponding to the major peak of the anti-IL-8 activity. The nature of the substance in SGE that was responsible for this effect remains unclear.
authors.affiliation: Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Sláviková
authors.forename: P
authors.initials: P
authors.lastname: Kocáková
authors.forename: M
authors.initials: M
authors.lastname: Slovák
authors.forename: I
authors.initials: I
authors.lastname: Vancová
authors.forename: V
authors.initials: V
authors.lastname: Hajnická
authors.forename: J
authors.initials: J
authors.lastname: Gasperík
authors.forename: N
authors.initials: N
authors.lastname: Fuchsberger
authors.forename: P A
authors.initials: PA
authors.lastname: Nuttall
mesh_terms: D000818:Animals; D002457:Cell Extracts / Q000494:pharmacology; D002458:Cell Fractionation; D002853:Chromatography, Liquid; D003870:Dermacentor / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D016209:Interleukin-8 / Q000302:isolation & purification / Q000378:metabolism; D007739:L Cells; D051379:Mice; D019251:Nucleocapsid / Q000096:biosynthesis* / Q000378:metabolism; D019590:Nucleocapsid Proteins; D012469:Salivary Glands / Q000737:chemistry* / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism*
chemical_list: D002457:Cell Extracts; D016209:Interleukin-8; D019590:Nucleocapsid Proteins