Interleukin (IL)-4 and IL-17 synergistically stimulate IL-6 secretion in human colonic myofibroblasts.
There is increasing evidence that interleukin (IL)-4 can aid in Th1-type inflammatory responses in chronic colitis models. In this study, we evaluated the effects of IL-4 and/or IL-17 on IL-6 secretion in human colonic myofibroblasts. IL-6 secretion was determined by ELISA and Northern blotting. IL-6 secretion was rapidly induced by either IL-4 or IL-17. IL-17 induced IL-6 mRNA expression within 1 h after stimulation, and reached a maximum at 3 h. IL-6 mRNA induction by IL-4 occurred more rapidly. A maximum induction of IL-6 mRNA by IL-4 was observed at 1 h after stimulation, and this was rapidly decreased. The combination of IL-4 plus IL-17 greatly enhanced IL-6 secretion and mRNA expression. In conclusion, IL-4, in particular IL-4 plus IL-17, induced IL-6 secretion in human colonic myofibroblasts. Th2 immune responses might play an important role in the pathogenesis of gut inflammation.
Andoh
Akira
A
Division of Gastroenterology, Shiga University of MEdical Science, Seta Tukinowa, Otsu, Japan. andoh@belle.shiga-med.ac.jp
Hata
Kazunori
K
Araki
Yoshio
Y
Fujiyama
Yoshihide
Y
Bamba
Tadao
T
D002478:Cells, Cultured; D003106:Colon / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D004357:Drug Synergism; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D015212:Inflammatory Bowel Diseases / Q000209:etiology / Q000276:immunology; D020381:Interleukin-17 / Q000008:administration & dosage*; D015847:Interleukin-4 / Q000008:administration & dosage*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D018836:Inflammation Mediators; D020381:Interleukin-17; D015850:Interleukin-6; D012333:RNA, Messenger; D015847:Interleukin-4