Glucose metabolism and regulation in lactating mink (Mustela vison)--effects of low dietary protein supply.
Eighteen lactating mink raising litters of 6 to 7 kits were fed ad libitum from parturition on diets with 32% of ME derived from protein and decreasing fat:carbohydrate ratios [high fat:low carbohydrate (HFLC): 67:1, medium fat:medium carbohydrate (MFMC): 52:16, low fat:high carbohydrate (LFHC): 37:31]. Four weeks post partum the dams were fitted with a jugular vein catheter, and the experiment started with a 3 hours fasting period, after which the dams were fed 210 kJ ME of the experimental diets. Blood samples were collected 10 and 5 min before feeding and 30, 60, 90, 120, 150 and 180 min postprandially. Two hours postprandially a single dose of 50 microCi U-14C-labelled glucose was administered to each dam and blood samples were collected 5, 10, 20, 30, 45 and 60 min after the tracer administration. Plasma concentrations of glucose and insulin 30 to 120 min postprandially were higher in dams fed the LFHC diet, than in dams fed the HFLC diet, values for dams fed the MFMC diet being intermediate. Plasma glucagon concentrations were not significantly affected by dietary treatment. The glucagon:insulin ratios decreased postprandially in all dams, the response being significant in dams fed the LFHC diet. Plasma concentrations of urea were not significantly affected by dietary treatment. Plasma FFA concentrations tended to increase postprandially in dams fed the HFLC diet. Glucose turnover rates were approximately 4.0% per min in all dams, irrespective of dietary treatment. However, the daily glucose flux was lower in dams fed the HFLC diet than in dams fed the LFHC diet, and tended to be lower than in dams fed the MFMC diet. In conclusion, a dietary protein supply of 32% of ME simultaneously with a carbohydrate supply of 16% or 31% of ME had no adverse effects on glucose homeostasis or glucose metabolism in lactating mink.
Fink
Rikke
R
Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, P.O. Box 50, DK-8830 Tjele, Denmark. Rikke.Fink@agrsci.dk
Børsting
Chr F
CF
Damgaard
Birthe Marie
BM
Rosted
Anne Katrine Lundegård
AK
D000821:Animal Feed; D000818:Animals; D019540:Area Under Curve; D001786:Blood Glucose / Q000378:metabolism; D002250:Carbon Radioisotopes; D018753:Diet, Protein-Restricted / Q000662:veterinary*; D004040:Dietary Carbohydrates / Q000008:administration & dosage* / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D005947:Glucose / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007328:Insulin / Q000097:blood; D007774:Lactation / Q000378:metabolism* / Q000502:physiology; D008907:Mink / Q000378:metabolism* / Q000502:physiology; D009751:Nutritional Requirements; D019518:Postprandial Period
D001786:Blood Glucose; D002250:Carbon Radioisotopes; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004044:Dietary Proteins; D007328:Insulin; D005947:Glucose