title: [CPP32 expression and its significance in multidrug-resistant tumor cells and their parent cells].
abstract: OBJECTIVE
  To study the expression difference of CPP32 in multidrug-resistant (mdr) tumor cells and their parent cells and to understand the possible effect of CPP32 in apoptosis induction in the 2 cell lines.
  
  
  METHODS
  Sequence analysis of CPP32 mRNA extracted form mdr gastric cancer cell line SGC7901/VCR08 and naive cell line SGC7901 was performed by means of reverse transcriptase-polymerase chain reaction (RT-PCR), and the protein expression of CPP32 in the 2 cell lines assayed by way of Western blotting.
  
  
  RESULTS
  The expressions of CPP32 mRNA was comparable in the 2 cell lines, and sequence analysis found consistent sequence of CPP32 in both cell lines with previous report. No differences was identified in the protein expression, either.
  
  
  CONCLUSIONS
  The apoptosis resistance of mdr cells is not related to the abnormality of CPP32 but the upstream of caspase, the fact of which indicates promising prospect of the research on reversion of mdr cells using CPP32 as target.
authors.affiliation: Institute of Digestive Disease of PLA, Nangfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Shao-Dong
authors.initials: SD
authors.lastname: Wang
authors.forename: Zhen-Shu
authors.initials: ZS
authors.lastname: Zhang
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Dian-Yuan
authors.initials: DY
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000502:physiology; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis*; D018432:Drug Resistance, Multiple / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases