title: Loss of heterozygosity at microsatellite markers from region p11-21 of chromosome 8 in microdissected breast tumor but not in peritumoral cells.
abstract: Alterations of chromosomal region 8p11-21 are very frequent in human cancers, and especially in breast cancer; yet, most of the genes involved have not been identified. We performed laser capture microdissection in a series of 52 consecutive breast tumor samples to obtain pure tumor cells without surrounding normal breast. To determine genomic subregions in which some of the cancer genes may be located, we conducted a search for loss of heterozygosity (LOH) at 13 microsatellite markers from this region. Two-thirds of the tumors showed LOH at least at one marker. Microdissection of pure tumor samples was helpful to precisely define four LOH subregions. No LOH was observed in the corresponding peritumoral tissues. We studied by immunohistochemistry (IHC) on tissue-microarrays the expression in the same tumors, of the protein product of three potential tumor genes lying close to or within the subregions of LOH. In most samples, the TACC1 gene product was downregulated in tumor cells as compared to normal cells. Our results show that the centromeric portion of chromosome arm 8p is frequently altered in breast tumor cells.
authors.affiliation: Departement d'Oncologie Moleculaire, Institut Paoli-Calmettes (IPC), Marseille, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Charafe-Jauffret
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Moulin
authors.forename: Christophe
authors.initials: C
authors.lastname: Ginestier
authors.forename: Didier
authors.initials: D
authors.lastname: Bechlian
authors.forename: Nathalie
authors.initials: N
authors.lastname: Conte
authors.forename: Jeannine
authors.initials: J
authors.lastname: Geneix
authors.forename: José
authors.initials: J
authors.lastname: Adélaïde
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Noguchi
authors.forename: Jacques
authors.initials: J
authors.lastname: Hassoun
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Jacquemier
authors.forename: Daniel
authors.initials: D
authors.lastname: Birnbaum
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry / Q000235:genetics*; D002898:Chromosomes, Human, Pair 8; D005260:Female; D005326:Fetal Proteins / Q000032:analysis; D005819:Genetic Markers; D006801:Humans; D007150:Immunohistochemistry; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D008869:Microtubule-Associated Proteins / Q000032:analysis; D008875:Middle Aged; D020890:Neuregulin-1 / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis
chemical_list: D005326:Fetal Proteins; D005819:Genetic Markers; D008869:Microtubule-Associated Proteins; D020890:Neuregulin-1; D009687:Nuclear Proteins; C120584:TACC1 protein, human