Properties of a chimeric simian-human immunodeficiency virus expressing an hybrid HIV-1 Nef/SIVmac Nef protein.
The nef genes of human and simian immunodeficiency viruses code for a membrane associated protein critical for AIDS development. SIVmac Nef presents C-terminal a 27 amino acid extension absent of HIV-1 Nef. To estimate the influence of this C-terminal domain on virus properties, we constructed viruses derived from SIVmac239 by replacing SIV nef with HIV-1 Lai nef gene (SHIV NefLai4) or with a sequence encoding a Nef fusion protein: HIV-1 Lai Nef/SIV Nef-Cterm (SHIV-Cterm). The recombinant viruses replicated efficiently in vitro in CEMx174 cells and in activated macaque PBMCs. The addition of SIV Nef C-terminal domain to HIV-1 Nef in SHIVNefLai4 did not change the in vitro properties of the chimeric virus, both viruses being more infectious than a nef deleted virus. Although the half-life of Nef fusion protein was augmented, SHIV-Cterm remained slightly less infectious than SIVmac239.
Bertsch
C
C
INSERM U544, Institut de Virologie, Université Louis Pasteur, Strasbourg, France.
Cluet
D
D
Beyer
C
C
Gloeckler
L
L
Cecile
A
A
Gut
J P
JP
Aubertin
A M
AM
D000595:Amino Acid Sequence; D000818:Animals; D015754:Genes, nef; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity; D008251:Macaca; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000472:pathogenicity; D054334:Viral Regulatory and Accessory Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D014779:Virus Replication
C429284:NEF protein, SIV; D011993:Recombinant Fusion Proteins; D054334:Viral Regulatory and Accessory Proteins