title: Expression of members of Wnt and Frizzled gene families in the postnatal rat cochlea.
abstract: The functioning of the mammalian cochlea is entirely based on its mechanical properties, which are supported by a highly complex tissue architecture resulting from the precise arrangement of sensory hair cells and non-sensory supporting cells. Growing evidence indicates that evolutionary conserved signaling pathways are involved in inner ear development and in the differentiation of its diverse cell types. We investigated whether members of the Wnt and Frizzled gene families, which play key roles in a wide variety of cellular and developmental processes, are expressed in the postnatal rat cochlea. A PCR screening of a rat cochlea cDNA library performed with degenerate primers allowed us to isolate five members of the Wnt gene family (RWnt-2B, -4, -5A, -5B, and -7A) and six members of the Frizzled gene family (Rfz1, Rfz2, Rfz3, Rfz4, Rfz6, Rfz9). In situ hybridization and immunocytochemistry experiments demonstrated that RWnt-4, -5B, -7A have distinct, although partly overlapping, expression patterns in the juvenile rat cochlea. These results suggest that the Wnt-Frizzled signaling pathway could be involved in several aspects of late cochlear differentiation and/or auditory function.
authors.affiliation: INSERM U254, Université Montpellier I, Montpellier, France. nicolas.daudet@cancer.org.uk
authors.forename: Nicolas
authors.initials: N
authors.lastname: Daudet
authors.forename: Chantal
authors.initials: C
authors.lastname: Ripoll
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Molès
authors.forename: Guy
authors.initials: G
authors.lastname: Rebillard
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000235:genetics*; D003051:Cochlea / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D051157:Frizzled Receptors; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D015723:Gene Library; D005820:Genetic Testing; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D007760:Labyrinth Supporting Cells / Q000166:cytology / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D043562:Receptors, G-Protein-Coupled; D017981:Receptors, Neurotransmitter / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D051153:Wnt Proteins; D060528:Wnt4 Protein; D029961:Zebrafish Proteins
chemical_list: D018076:DNA, Complementary; C489740:FZD1 protein, human; D051157:Frizzled Receptors; C489739:Fzd1 protein, mouse; D006023:Glycoproteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D017981:Receptors, Neurotransmitter; D014157:Transcription Factors; C555581:WNT4 protein, human; D051153:Wnt Proteins; D060528:Wnt4 Protein; C555582:Wnt4 protein, mouse; C555583:Wnt4 protein, rat; C468567:Wnt5b protein, mouse; C490508:Wnt7a protein, mouse; C490507:Wnt7a protein, rat; D029961:Zebrafish Proteins; C078603:Fzd1 protein, rat