title: Optimization of media by evolutionary algorithms for production of polyols.
abstract: Biotransformation of sucrose-based medium to polyols has been reported for the first time using osmophilic yeast, Hansenula anomala. A new, real coded evolutionary algorithm was developed for optimization of fermentation medium in parallel shake-flask experiments. By iteratively employing the nature-inspired techniques of selection, crossover, and mutation for a fixed number of generations, the algorithm obtains the optimal values of important process variables, namely, inoculum size and sugar, yeast extract, urea, and MgSO4 concentrations. Maximum polyols yield of 76.43% has been achieved. The method is useful for reducing the overall development time to obtain an efficient fermentation process.
authors.affiliation: Department of Alcohol Technology, Vasantdada Sugar Institute, Pune, India.
authors.forename: S V
authors.initials: SV
authors.lastname: Patil
authors.forename: V K
authors.initials: VK
authors.lastname: Jayaraman
authors.forename: B D
authors.initials: BD
authors.lastname: Kulkarni
mesh_terms: D000465:Algorithms; D001711:Biotransformation; D003470:Culture Media; D005285:Fermentation; D008278:Magnesium Sulfate / Q000494:pharmacology; D008957:Models, Genetic; D010843:Pichia / Q000254:growth & development / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology; D014508:Urea / Q000378:metabolism / Q000494:pharmacology
chemical_list: D003470:Culture Media; D011108:Polymers; C024617:polyol; D013395:Sucrose; D008278:Magnesium Sulfate; D014508:Urea