Distribution of thymidine phosphorylase activity in gastric carcinoma and adjacent normal gastric tissue.
OBJECTIVE
Thymidine phosphorylase was reported to be identical to the angiogenic factor, platelet-derived endothelial cell growth factor. In this study we investigated the distribution of thymidine phosphorylase activity in human gastric carcinoma or normal gastric tissue using the ELISA system.


METHODS
A longitudinal slice in the center of the gastric carcinoma of resected specimens from 6 patients with gastric carcinoma was used, and thymidine phosphorylase activity was mapped in each case.


RESULTS
In all cases, the thymidine phosphorylase activities were significantly higher in tumors than adjacent normal gastric tissues. The amount and distribution of thymidine phosphorylase activity were different between intestinal-type and diffuse-type carcinoma. The thymidine phosphorylase activities in the invasive front of tumor were significantly lower than those in the other part in intestinal-type carcinoma.


CONCLUSIONS
The ELISA system used in this study proved useful for the determination of thymidine phosphorylase activities in tissue sections.
Sugiyama
Atsushi
A
First Department of Surgery, Shinshu University, School of Medicine, Matsumoto, Japan. sugiyama@hsp.md.shinshu-u.ac.jp
Maruta
Fukuto
F
Tsuzuki
Shigetoshi
S
Murakami
Maki
M
Kawasaki
Seiji
S
D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000201:enzymology*; D013939:Thymidine Phosphorylase / Q000378:metabolism*
D013939:Thymidine Phosphorylase