title: Dependence of Na+-K+ pump current-voltage relationship on intracellular Na+, K+, and Cs+ in rabbit cardiac myocytes.
abstract: To examine effects of cytosolic Na+, K+, and Cs+ on the voltage dependence of the Na+-K+ pump, we measured Na+-K+ pump current (Ip) of ventricular myocytes voltage-clamped at potentials (Vm) from 100 to +60 mV. Superfusates were designed to eliminate voltage dependence at extracellular pump sites. The cytosolic compartment of myocytes was perfused with patch pipette solutions with a Na+ concentration ([Na]pip) of 80 mM and a K+ concentration from 0 to 80 mM or with solutions containing Na+ in concentrations from 0.1 to 100 mM and K+ in a concentration of either 0 or 80 mM. When [Na]pip was 80 mM, K+ in pipette solutions had a voltage-dependent inhibitory effect on Ip and induced a negative slope of the Ip-Vm relationship. Cs+ in pipette solutions had an effect on Ip qualitatively similar to that of K+. Increases in Ip with increases in [Na]pip were voltage dependent. The dielectric coefficient derived from [Na]pip-Ip relationships at the different test potentials was 0.15 when pipette solutions included 80 mM K+ and 0.06 when pipette solutions were K+ free.
authors.affiliation: Department of Cardiology, Royal North Shore Hospital, St. Leonards, NSW 2065, Australia.
authors.forename: Peter S
authors.initials: PS
authors.lastname: Hansen
authors.forename: Kerrie A
authors.initials: KA
authors.lastname: Buhagiar
authors.forename: Benjamin Y
authors.initials: BY
authors.lastname: Kong
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Clarke
authors.forename: David F
authors.initials: DF
authors.lastname: Gray
authors.forename: Helge H
authors.initials: HH
authors.lastname: Rasmussen
mesh_terms: D000818:Animals; D002586:Cesium / Q000493:pharmacokinetics*; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000201:enzymology; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000493:pharmacokinetics*; D011817:Rabbits; D012964:Sodium / Q000493:pharmacokinetics*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D002586:Cesium; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium