Klebsiella pneumoniae KE-1 degrades endosulfan without formation of the toxic metabolite, endosulfan sulfate.
For bioremediation of toxic endosulfan, endosulfan degradation bacteria, which do not form toxic endosulfan sulfate, were isolated from various soil samples using endosulfan as sole carbon and energy source. Among the 40 isolated bacteria, strain KE-1, which was identified as Klebsiella pneumoniae by physiological and 16S rDNA sequence analysis, showed superior endosulfan degradation activity. Analysis of culture pH, growth, free sulfate and endosulfan and its metabolites demonstrated that KE-1 biologically degrades 8.72 microg endosulfan ml(-1) day(-1) when incubated with 93.9 microg ml(-1) endosulfan for 10 days without formation of toxic endosulfan sulfate. Our results suggest that K. pneumoniae KE-1 degraded endosulfan by a non-oxidative pathway and that strain KE-1 has potential as a biocatalyst for endosulfan bioremediation.
Kwon
Gi-Seok
GS
The School of Bioresource Science, Andong National University, South Korea. gskwon@andong.ac.kr
Kim
Jang-Eok
JE
Kim
Taek-Kyum
TK
Sohn
Ho-Yong
HY
Koh
Sung-Cheol
SC
Shin
Kee-Sun
KS
Kim
Dong-Geol
DG
D001673:Biodegradation, Environmental; D004275:DNA, Ribosomal / Q000032:analysis; D004726:Endosulfan / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism
D004275:DNA, Ribosomal; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D013431:Sulfates; D004726:Endosulfan