Gelatinase activities in the airways of premature infants and development of bronchopulmonary dysplasia.
Matrix-degrading metalloproteinases may play a role in the pathophysiology of bronchopulmonary dysplasia (BDP). We, therefore, evaluated correlations between gelatinase activities [metalloproteinase (MMP)-2 and MMP-9] or tissue inhibitor of metalloproteinase (TIMP)-1 levels present in the airways during the initial phase of hyaline membrane disease and the onset of BPD. Tracheal aspirates were obtained within 6 h of birth (day 0) from 64 intubated neonates with a gestational age < or =30 wk. Forty-five neonates were resampled on day 3 or 5. Total MMP-2 level measured by zymography fell with time, whereas total MMP-9 level and TIMP-1 levels, assayed by ELISA, increased; the MMP-9 increase correlated with the increase in airway inflammatory cell numbers. Among the parameters measured on day 0, 3, or 5, lower total MMP-2 level, lower birth weight, and higher fraction of inspired oxygen on day 0 were significantly and independently associated with the development of BPD. In conclusion, MMP-9 level and TIMP-1 levels increased after birth but are not linked to BPD outcome. In contrast, low MMP-2 level at birth is strongly associated with the development of BPD.
Danan
Claude
C
Unité de Réanimation Néonatale, Centre Hospitalier Intercommunal de Créteil, France.
Jarreau
Pierre-Henri
PH
Franco
Marie-Laure
ML
Dassieu
Gilles
G
Grillon
Christophe
C
Abd Alsamad
Issam
I
Lafuma
Chantal
C
Harf
Alain
A
Delacourt
Christophe
C
D001997:Bronchopulmonary Dysplasia / Q000503:physiopathology*; D004789:Enzyme Activation; D018093:Gelatinases / Q000378:metabolism*; D006801:Humans; D006819:Hyaline Membrane Disease / Q000201:enzymology / Q000503:physiopathology; D007231:Infant, Newborn; D007234:Infant, Premature; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009130:Muscle, Smooth / Q000201:enzymology; D012129:Respiratory Function Tests; D013997:Time Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D014132:Trachea / Q000201:enzymology*
D019715:Tissue Inhibitor of Metalloproteinase-1; D018093:Gelatinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9