title: Outbreak of jaundice and hemorrhagic fever in the Southeast of Brazil in 2001: detection and molecular characterization of yellow fever virus.
abstract: Between January and March 2001, an outbreak of jaundice and hemorrhagic fever occurred in the state of Minas Gerais, Southeast region of Brazil, in which a mortality rate of 53% was reported. Seroconversion, virus isolation, histopathological and immunohistochemical findings, and reverse transcription-polymerase chain reaction (RT-PCR) identified yellow fever virus (YFV) as the etiological agent responsible for the outbreak. Partial nucleotide sequence analysis from a fragment of the YFV genome spanning parts of nonstructural (NS) 5 gene and 3' noncoding region (3' UTR) showed that the YFV involved in this outbreak belongs to South American genotype I and differs from the Brazilian virus identified in 1996.
authors.affiliation: Laboratório de Flavivírus, Departamento de Virologia, Instituto Oswaldo Cruz, Fiocruz, RJ, Brasil. ambispo@gene.dbbm.fiocruz.br
authors.forename: A M B
authors.initials: AM
authors.lastname: de Filippis
authors.forename: R M R
authors.initials: RM
authors.lastname: Nogueira
authors.forename: H G
authors.initials: HG
authors.lastname: Schatzmayr
authors.forename: D S
authors.initials: DS
authors.lastname: Tavares
authors.forename: A V
authors.initials: AV
authors.lastname: Jabor
authors.forename: S C M
authors.initials: SC
authors.lastname: Diniz
authors.forename: J C
authors.initials: JC
authors.lastname: Oliveira
authors.forename: E
authors.initials: E
authors.lastname: Moreira
authors.forename: M P
authors.initials: MP
authors.lastname: Miagostovich
authors.forename: E V
authors.initials: EV
authors.lastname: Costa
authors.forename: R
authors.initials: R
authors.lastname: Galler
mesh_terms: D000595:Amino Acid Sequence; D001938:Brazil / Q000453:epidemiology; D004196:Disease Outbreaks; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D007565:Jaundice / Q000453:epidemiology* / Q000209:etiology / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*; D016036:Seroepidemiologic Studies; D015004:Yellow Fever / Q000453:epidemiology* / Q000209:etiology / Q000821:virology; D015005:Yellow fever virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D007075:Immunoglobulin M; D012367:RNA, Viral