title: [Cloning E. coli mRNAs with poly (A) tails by restriction digest polymerase chain reaction].
abstract: OBJECTIVE
  To investigate the polyadenylation at the 3' terminal of the mRNAs in E.coli.
  
  
  METHODS
  mRNAs of E.coli was enriched from total RNA with oligo (dT)-cellulose, and reverse transcription was performed using oligo(dT)18 as primer prior to synthesis of double strands cDNA which was digested with Sau 3A I to produce multiple gene fragments that were then ligated with adapters. Restriction digest-polymerase chain reaction (RD-PCR) was employed to divide the fragments into 10 groups using 10 different combinations of the 4 primers, and the products were cloned into T-vectors.
  
  
  RESULTS
  More than 100 gene fragments were cloned, 30 of which were sequenced.
  
  
  CONCLUSIONS
  Polyadenylation of E.coli mRNA is not a biochemical curiosity, and very likely, it is a general attribute of the mRNAs of bacteria.
authors.affiliation: Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
authors.forename: Zi-You
authors.initials: ZY
authors.lastname: Hu
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Ma
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Song
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Wu
authors.forename: Qiu-Ye
authors.initials: QY
authors.lastname: Guo
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Peng
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D011061:Poly A; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases