V-1, a catecholamine biosynthesis regulatory protein, positively controls catecholamine secretion in PC12D cells.
Stably transfected PC12D cell lines overexpressing a catecholamine biosynthesis regulatory protein, V-1, were used to examine the functional role of V-1 in catecholamine secretion. High K(+)-induced dopamine secretion in V-1 overexpressing clones was shown to be markedly potentiated compared with control clones carried with a vector alone. As assayed intracellular calcium concentration ([Ca(2+)](i)) using fura-PE3, V-1 overexpression was observed to enhance high K(+)-elicited [Ca(2+)](i) elevation. Electron microscopic analysis revealed an increase in dense-cored vesicle formation by V-1 overexpression. These results suggest that the enhancement of high K(+)-induced dopamine secretion by V-1 overexpression results from the potentiation of high K(+)-induced [Ca(2+)](i) elevation and the increase in the number of dense-cored vesicles.
Yamakuni
Tohru
T
Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan. yamakuni@mail.pharm.tohoku.ac.jp
Yamamoto
Toshifumi
T
Ishida
Yukisato
Y
Yamamoto
Hideko
H
Song
Si Young
SY
Adachi
Eijiro
E
Hiwatashi
Yusuke
Y
Ohizumi
Yasushi
Y
D000818:Animals; D015153:Blotting, Western; D004298:Dopamine / Q000096:biosynthesis* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats
D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C065700:myotrophin; D004298:Dopamine