title: PCR and molecular detection for differentiating Vibrio species.
abstract: Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.
authors.affiliation: Centre for Marine Biodiversity and Biotechnology (CMBB), Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland, UK. olivier.sparagano@ncl.ac.uk
authors.forename: O A E
authors.initials: OA
authors.lastname: Sparagano
authors.forename: P A W
authors.initials: PA
authors.lastname: Robertson
authors.forename: I
authors.initials: I
authors.lastname: Purdom
authors.forename: J
authors.initials: J
authors.lastname: McInnes
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: D-H
authors.initials: DH
authors.lastname: Yu
authors.forename: Z-J
authors.initials: ZJ
authors.lastname: Du
authors.forename: H-S
authors.initials: HS
authors.lastname: Xu
authors.forename: B
authors.initials: B
authors.lastname: Austin
mesh_terms: D000818:Animals; D017756:Aquaculture; D001483:Base Sequence; D003257:Consumer Product Safety; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D018076:DNA, Complementary; D005393:Fish Diseases / Q000453:epidemiology / Q000821:virology; D005399:Fishes; D006801:Humans; D007448:Invertebrates / Q000821:virology; D008322:Mammals / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity; D016415:Sequence Alignment / Q000662:veterinary; D013045:Species Specificity; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D014735:Vibrio Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D015047:Zoonoses
chemical_list: D004269:DNA, Bacterial; D018076:DNA, Complementary; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S