High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma.
A HPLC assay was developed to determine IDN 5390, a new paclitaxel analogue, in mouse plasma. The method involves solid-phase extraction from cyano cartridges (recovery >80%), HPLC separation on Symmetry C(18) (4.6 x 150 mm), on isocratic mobile phase of water-acetonitrile-acetic acid (49:50:1) and detection at 227 nm. Retention times of IDN 5390 and IDN 5517 (internal standard, I.S.) were 9.1 and 10.5 min, respectively. The assay was linear from 0.05 to 5 micro g/ml (r(2)>or=0.995), showed intra- and inter-day precision within 1.0 and 6.2%, and accuracy of 94.7-106.8%. LOQ was 0.050 micro g/ml. Using this method IDN 5390 pharmacokinetics was determined in mice.
Zaffaroni
M
M
Department of Oncology, Istituto di Ricerche Farmacologiche "Mario Negri", Via Eritrea 62, 20157, Milan, Italy.
Frapolli
R
R
Colombo
T
T
Fruscio
R
R
Bombardelli
E
E
Morazzoni
P
P
Riva
A
A
D'Incalci
M
M
Zucchetti
M
M
D000818:Animals; D001952:Bridged-Ring Compounds / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D051379:Mice; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D043823:Taxoids
D001952:Bridged-Ring Compounds; C459840:IDN 5390; D043823:Taxoids; D017239:Paclitaxel