Brevican isoforms associate with neural membranes.
Brevican is a neural-specific proteoglycan of the brain extracellular matrix, which is particularly abundant in the terminally differentiated CNS. It is expressed by neuronal and glial cells, and as a component of the perineuronal nets it decorates the surface of large neuronal somata and primary dendrites. One brevican isoform harbors a glycosylphosphatidylinositol anchor attachment site and, as shown by ethanolamine incorporation studies, is indeed glypiated in stably transfected HEK293 cells as well as in oligodendrocyte precursor Oli-neu cells. The major isoform is secreted into the extracellular space, although a significant amount appears to be tightly attached to the cell membrane, as it floats up in sucrose gradients. Flotation is sensitive to detergent treatment. Brevican is most prominent in the microsomal, light membrane and synaptosomal fractions of rat brain membrane preparations. The association with the particulate fraction is in part sensitive to chondroitinase ABC and phosphatidylinositol-specific phospholipase C treatment. Furthermore, brevican staining on the surface of hippocampal neurons in culture is diminished after hyaluronidase or chondroitinase ABC treatment. Taken together, this could provide a mechanism by which perineuronal nets are anchored on neuronal surfaces.
Seidenbecher
Constanze I
CI
AG Molecular Mechanisms of Plasticity, Department of Neurochemistry/Molecular Biology, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany. seidenc@ifn-magdeburg.de
Smalla
Karl-Heinz
KH
Fischer
Nora
N
Gundelfinger
Eckart D
ED
Kreutz
Michael R
MR
D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry; D058581:Brevican; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D019856:Ethanolamine / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000494:pharmacology; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D037181:Lectins, C-Type; D008861:Microsomes / Q000737:chemistry; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D012995:Solubility / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013347:Subcellular Fractions / Q000737:chemistry; D013574:Synaptosomes / Q000737:chemistry
C546939:Bcan protein, rat; D058581:Brevican; D011508:Chondroitin Sulfate Proteoglycans; D017261:Glycosylphosphatidylinositols; D037181:Lectins, C-Type; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D019856:Ethanolamine; D006026:Glycoside Hydrolases