title: Folic acid, ascorbic acid and sodium selenite restore the motility of Dictyostelium discoideum inhibited by triethyllead.
abstract: The effect of triethyllead (TriEL) on motile activity, structure of cytoskeleton and chemotaxis of Dictyostelium discoideum amoebae in developing concentration gradients of folic acid (FA) and cAMP has been studied. It was observed that 3 microM TriEL had little or no effect on locomotion and chemotactic response of cells, whereas 5 microM TriEL strongly reduced the motile activity of Dictyostelium discoideum amoebae and inhibited their chemotaxis towards cAMP, but not towards FA. FA was found to restore the motile activity of Dictyostelium discoideum, inhibited by TriEL. A similar effect was observed in the presence of other antioxidants, i.e. ascorbic acid and sodium selenite, suggesting that oxidative stress may be involved in the action of TriEL. Moreover, the treatment of Dictyostelium amoebae with 5 microM TriEL caused disruption of microtubules while 3 microM TriEL had little effect on their structure. FA caused restoration of microtubules only in some cells within 1 h of incubation, i.e. when the directional movement of cells towards this chemoattractant was already observed. However, their organization was significantly different from that observed in the untreated cells, suggesting that microtubule undisturbed organisation may be not necessary for Dictyostelium discoideum amoebae locomotion and chemotaxis
authors.affiliation: Department of Cell Biology, The J. Zurzycki Institute of Molecular Biology and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland. jola@mol.uj.edu.pl
authors.forename: Jolanta
authors.initials: J
authors.lastname: Sroka
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Madeja
authors.forename: Marta
authors.initials: M
authors.lastname: Michalik
authors.forename: Stanisław
authors.initials: S
authors.lastname: Przestalski
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Korohoda
mesh_terms: D000199:Actins / Q000187:drug effects / Q000648:ultrastructure; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000633:toxicity; D002633:Chemotaxis / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology; D004023:Dictyostelium / Q000187:drug effects*; D005455:Fluorescent Antibody Technique; D005492:Folic Acid / Q000494:pharmacology*; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure; D009942:Organometallic Compounds / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D018038:Sodium Selenite / Q000494:pharmacology*
chemical_list: D000199:Actins; D002630:Chemotactic Factors; D009942:Organometallic Compounds; C010360:triethyllead; D005492:Folic Acid; D000242:Cyclic AMP; D018038:Sodium Selenite; D001205:Ascorbic Acid