Detection of connective tissue growth factor in human aqueous humor.
OBJECTIVE
Connective tissue growth factor (CTGF) has been shown to be substantially involved in various processes of fibrosis. We investigated if CTGF is present in aqueous humor (AH).


METHODS
Samples from AH were collected from 10 volunteers during cataract surgery. Specific ELISA analysis was performed with goat IGG against human CTGF.


RESULTS
CTGF was above the detection limit of the assay in 80% of the samples. The concentration of CTGF in the anterior chamber fluid was 1.24 +/- (SD) 0.26 ng/ml.


CONCLUSIONS
CTGF is present in a majority of AH samples, possibly representing a constant component in this fluid. The origin and physiological importance of CTGF is yet unclear. The involvement of CTGF in processes of intraocular fibrosis and wound healing is possible.
van Setten
G-B
GB
St. Eriks Eye Clinic, Karolinska Institutet, Stockholm, Sweden. g.van.setten@sankterik.se
Blalock
Timothy D
TD
Grotendorst
Gary
G
Schultz
Gregory S
GS
D000368:Aged; D001082:Aqueous Humor / Q000378:metabolism*; D055513:Connective Tissue Growth Factor; D005260:Female; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration
C526317:CCN2 protein, human; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D055513:Connective Tissue Growth Factor