Genetic analysis and functional characterization of the Streptococcus pneumoniae vic operon.
The vic two-component signal transduction system of Streptococcus pneumoniae is essential for growth. The vic operon comprises three genes encoding the following: VicR, a response regulator of the OmpR family; VicK, its cognate histidine kinase; and VicX, a putative protein sharing 55% identity to the predicted product (YycJ) of an open reading frame in the Bacillus subtilis genome. We show that not only is vic essential for viability but it also influences virulence and competence. A putative transcriptional start site for the vic operon was mapped 16 bp upstream of the ATG codon of vicR. Only one transcript of 2.9 kb, encoding all three genes, was detected by Northern blot analysis. VicK, an atypical PAS domain-containing histidine kinase, can be autophosphorylated in vitro, and VicR functions in vitro as a phospho-acceptor protein. (PAS is an acronym formed from the names of the proteins in which the domains were first recognized: the Drosophila period clock protein [PER], vertebrate aryl hydrocarbon receptor nuclear translocator [ARNT], and Drosophila single-minded protein [SIM].) PAS domains are commonly involved in sensing intracellular signals such as redox potential, which suggests that the signal for vic might also originate in the cytoplasm. Growth rate, competence, and virulence were monitored in strains with mutations in the vic operon. Overexpression of the histidine kinase, VicK, resulted in decreased virulence, whereas the transformability of a null mutant decreased by 3 orders of magnitude.
Wagner
Christian
C
F. Hoffmann-La Roche Ltd., 4070 Basel. Division of Microbiology, Biozentrum, University of Basel, 4056 Basel, Switzerland.
Saizieu Ad
Antoine de
Ad
Schönfeld
Hans-Joachim
HJ
Kamber
Markus
M
Lange
Roland
R
Thompson
Charles J
CJ
Page
Malcolm G
MG
D000818:Animals; D001426:Bacterial Proteins; D005260:Female; D000071677:Histidine Kinase; D051379:Mice; D008810:Mice, Inbred C57BL; D009876:Operon / Q000502:physiology*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D013296:Streptococcus pneumoniae / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D015534:Trans-Activators / Q000235:genetics*; D014774:Virulence
D001426:Bacterial Proteins; D015534:Trans-Activators; C112177:osmolarity response regulator proteins; D011494:Protein Kinases; D000071677:Histidine Kinase