title: [Effect of nitric oxide on the invasive ability of human osteosarcoma through a filter membrane: an in vitro study].
abstract: OBJECTIVE
  To study the effect of nitric oxide on the in vitro invasiveness of human osteosarcoma cell line (OS-732).
  
  
  METHODS
  In vitro cultured OS-732 cell suspensions containing different concentrations of sodium nitroprusside (SNP) or PBS (control group) were used to assess the in vitro invasiveness of the tumor cells utilizing Boyden's chamber assay. The tumor cells growing across the filter membrane were counted by means of HE staining and compared between the groups.
  
  
  RESULTS
  With the increase of SNP concentration in the cell suspension, the number of tumor cells growing across the membrane tended to decrease. With the exception of the cell suspension containing 500 micromol/L SNP that had a cell number across the membrane significantly different from those of any other groups, significant difference was not observed between any adjacent groups. Between the groups with SNP concentrations that did not come adjacent, however, significant differences were noted.
  
  
  CONCLUSIONS
  Nitric oxide can depress the invasiveness of OS-732 cells across the membrane in vitro.
authors.affiliation: Department of Orthopaediatrics, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
authors.forename: Xin-Yu
authors.initials: XY
authors.lastname: Zhang
authors.forename: An-Min
authors.initials: AM
authors.lastname: Jin
authors.forename: Zhan-Jun
authors.initials: ZJ
authors.lastname: Shi
authors.forename: Ping
authors.initials: P
authors.lastname: Lu
mesh_terms: D016475:3T3 Cells; D000704:Analysis of Variance; D000818:Animals; D006801:Humans; D051379:Mice; D009361:Neoplasm Invasiveness; D009569:Nitric Oxide / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D012516:Osteosarcoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide