title: Characterization of the tetracycline resistance plasmid pMD5057 from Lactobacillus plantarum 5057 reveals a composite structure.
abstract: The 10,877bp tetracycline resistance plasmid pMD5057 from Lactobacillus plantarum 5057 was completely sequenced. The sequence revealed a composite structure containing DNA from up to four different sources. The replication region had homology to other plasmids of lactic acid bacteria while the tetracycline resistance region, containing a tet(M) gene, had high homology to sequences from Clostridium perfringens and Staphylococcus aureus. Within the tetracycline resistance region a Lactobacillus IS-element was found. The remaining part of the plasmid contained three open reading frames with unknown functions. The composite structure with several truncated genes suggests a recent assembly of the plasmid. This is the first sequence of an antibiotic resistance plasmid isolated from L. plantarum.
authors.affiliation: Applied Biotechnology, Identification Section, Chr. Hansen A/S, Bøge Allé 10-12, 2970 Hørsholm, Denmark. morten.danielsen@dk.chr-hansen.com
authors.forename: Morten
authors.initials: M
authors.lastname: Danielsen
mesh_terms: D001483:Base Sequence; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D023061:Gene Order; D005798:Genes, Bacterial / Q000235:genetics; D007778:Lactobacillus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification; D013752:Tetracycline / Q000494:pharmacology*
chemical_list: D004269:DNA, Bacterial; D013752:Tetracycline