title: Ssdp proteins interact with the LIM-domain-binding protein Ldb1 to regulate development.
abstract: The LIM-domain-binding protein Ldb1 is a key factor in the assembly of transcriptional complexes involving LIM-homeodomain proteins and other transcription factors that regulate animal development. We identified Ssdp proteins (previously described as sequence-specific, single-stranded-DNA-binding proteins) as components of Ldb1-associated nuclear complexes in HeLa cells. Ssdp proteins are associated with Ldb1 in a variety of additional mammalian cell types. This association is specific, does not depend on the presence of nucleic acids, and is functionally significant. Genes encoding Ssdp proteins are well conserved in evolution from Drosophila to humans. Whereas the vertebrate Ssdp gene family has several closely related members, the Drosophila Ssdp gene is unique. In Xenopus, Ssdp encoded by Drosophila Ssdp or mouse Ssdp1 mRNA enhances axis induction by Ldb1 in conjunction with the LIM-homeobox gene Xlim1. Furthermore, we were able to demonstrate an interaction between Ssdp and Chip (the fly homolog of Ldb1) in Drosophila wing development. These findings indicate functional conservation of Ssdp as a cofactor of Ldb1 during invertebrate and vertebrate development.
authors.affiliation: Laboratory of Mammalian Genes and Development, and Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20814, USA.
authors.forename: Lan
authors.initials: L
authors.lastname: Chen
authors.forename: Daniel
authors.initials: D
authors.lastname: Segal
authors.forename: Neil A
authors.initials: NA
authors.lastname: Hukriede
authors.forename: Alexandre V
authors.initials: AV
authors.lastname: Podtelejnikov
authors.forename: Dashzeveg
authors.initials: D
authors.lastname: Bayarsaihan
authors.forename: James A
authors.initials: JA
authors.lastname: Kennison
authors.forename: Vasily V
authors.initials: VV
authors.lastname: Ogryzko
authors.forename: Igor B
authors.initials: IB
authors.lastname: Dawid
authors.forename: Heiner
authors.initials: H
authors.lastname: Westphal
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D060588:LIM Domain Proteins; D060850:LIM-Homeodomain Proteins; D051379:Mice; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases; D014709:Vero Cells; D014981:Xenopus / Q000196:embryology; D029867:Xenopus Proteins; D060589:Zyxin
chemical_list: C108740:Chi protein, Drosophila; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; C486312:LDB1 protein, human; C085473:LHX1 protein, human; D060588:LIM Domain Proteins; D060850:LIM-Homeodomain Proteins; C102084:Ldb1 protein, mouse; C543964:Lhx1 protein, Xenopus; C499523:Lhx1 protein, mouse; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; C504091:NABP2 protein, human; C504094:SSBP3 protein, human; C504092:Ssbp2 protein, mouse; C504093:Ssbp3 protein, mouse; C520429:Ssdp protein, Drosophila; D014157:Transcription Factors; C486314:XLdb1 protein, Xenopus; D029867:Xenopus Proteins; C499520:Zyx protein, Drosophila; D060589:Zyxin; C467728:STUB1 protein, human; D044767:Ubiquitin-Protein Ligases