High-dose glucocorticoids inhibit proliferation of rat olfactory epithelium.
Glucocorticoids (GCs) are commonly prescribed for treatment of olfactory dysfunction. However, the effects of GCs on olfactory epithelium are not well known. We investigated the effects of high-dose GCs on proliferating cells of olfactory epithelium. Five adult male rats (300 g) received a single daily subcutaneous dose of vehicle containing 0.3 mg dexamethasone (DEX) for 9 days (DEX+ group), and a control group received vehicle alone (DEX- group). We compared sections from the two groups for numbers of Ki67-positive cells. The mean number of Ki67-positive cells per 500 olfactory epithelial cells was 9.6 for the DEX+ group and 58 for the DEX- group (significant difference). We conclude that high-dose GC suppressed proliferation of olfactory epithelium. We suggest that high-dose GC suppresses cytokines and growth factors, resulting in secondary suppression of proliferating ability.
Ohta
Yasushi
Y
Department of Otolaryngology-Head and Neck Surgery, Jichi Medical School, Tochigi, Japan.
Takanosawa
Minako
M
Matumoto
Chiori
C
Makino
Nobuko
N
Ishimoto
Shinichi
S
Ichimura
Keiichi
K
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology*; D005938:Glucocorticoids / Q000008:administration & dosage / Q000494:pharmacology*; D007150:Immunohistochemistry; D007279:Injections, Subcutaneous; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D009831:Olfactory Mucosa / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D019394:Ki-67 Antigen; D003907:Dexamethasone