title: Differential regulation of the two metallothionein genes in common carp.
abstract: The expression of two metallothionein (MT) genes was followed in carp (Cyprimus carpio) in vivo during exposure to As and Cu. Changes in the levels of MT-1 and MT-2 mRNA in the liver and kidney were detected by semi-quantitative RT-PCR. The inducibility of the two MT isoforms was tissue- and metal-specific. Regardless of whether As or Cu was applied, the liver was more responsive than the kidney. Copper influenced the expression of both isoforms: the MT-1 and MT-2 mRNA levels increased in both the liver and the kidney in a time- and dose-dependent manner. Arsenic affected mostly the MT-2 expression, while the level of the MT-1 transcript did not change significantly in either organ.
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Szeged, Hungary. hermesz@bio.u-szeged.hu
authors.forename: Edit
authors.initials: E
authors.lastname: Hermesz
authors.forename: Anett P
authors.initials: AP
authors.lastname: Gazdag
authors.forename: K Said
authors.initials: KS
authors.lastname: Ali
authors.forename: J
authors.initials: J
authors.lastname: Nemcsók
authors.forename: Magdolna
authors.initials: M
authors.lastname: Abrahám
mesh_terms: D000818:Animals; D001151:Arsenic / Q000494:pharmacology; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D003300:Copper / Q000494:pharmacology; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D003300:Copper; D008668:Metallothionein; D001151:Arsenic