Preservation of ischemia and isoflurane-induced preconditioning after brain death in rabbit hearts.
We sought to determine whether brain death-induced catecholamine release preconditions the heart, and if not, whether it precludes further protection by repetitive ischemia or isoflurane. Anesthetized rabbits underwent 30 min of coronary occlusion and 4 h of reperfusion. The effect on infarct size of either no intervention (controls), ischemic preconditioning (IPC), or isoflurane inhalation (Iso) was evaluated with or without previous brain death (BD) induced by subdural balloon inflation. Plasma catecholamine levels were measured at several time points. Although it dramatically increase plasma catecholamine levels, BD failed to reduce infarct size that averaged 0.49 +/- 0.34 without BD versus 0.45 +/- 0.27 g with BD. IPC and Iso, alone as well as after BD, significantly reduced infarct size that averaged 0.11 +/- 0.04, 0.21 +/- 0.15, 0.10 +/- 0.09, and 0.22 +/- 0.10 g in IPC, Iso, BD + IPC, and BD + Iso groups, respectively (means +/- SD, P < 0.05 vs. controls). BD-induced catecholamines "storm" does not precondition the rabbit heart that however retains the ability to be protected by repetition of brief ischemia or isoflurane inhalation.
Chiari
Pascal
P
Service d'Anesthésie Réanimation, Hôpital Cardiologique Louis Pradel, BP Lyon-Montchat, 69394 Lyon Cedex 03, France. p.chiari@vnumail.com
Piriou
Vincent
V
Hadour
Guylaine
G
Rodriguez
Claire
C
Loufouat
Joseph
J
Lehot
Jean-Jacques
JJ
Ovize
Michel
M
Ferrera
René
R
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure; D001926:Brain Death; D002395:Catecholamines / Q000097:blood; D005260:Female; D006339:Heart Rate; D019157:Ischemic Preconditioning, Myocardial; D007530:Isoflurane / Q000494:pharmacology*; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009203:Myocardial Infarction / Q000401:mortality / Q000473:pathology / Q000503:physiopathology; D017202:Myocardial Ischemia / Q000401:mortality / Q000473:pathology / Q000503:physiopathology*; D015221:Potassium Channels / Q000378:metabolism; D011817:Rabbits
D018685:Anesthetics, Inhalation; D002395:Catecholamines; D015221:Potassium Channels; D007530:Isoflurane