Effects of stereochemistry of sugars on protein stabilities.
We investigated thermal stabilities of four proteins in the presence of four kinds of sugars to analyze the mechanism of stabilization of proteins by additives. These proteins were stabilized by the addition of sugars, and the degree of stabilization correlated to the partial molar isentropic compressibility of the sugar.
Masumoto
Kiyonari
K
Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Ueda
Tadashi
T
Nagata
Makiko
M
Yamada
Yuko
Y
Yoshida
Yuichiro
Y
Hashimoto
Yoshio
Y
Imoto
Taiji
T
D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000737:chemistry*; D004795:Enzyme Stability; D006358:Hot Temperature; D007700:Kinetics; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry*
D002241:Carbohydrates; D011506:Proteins