Desferrioxamine decreases NAD redox potential of intact red blood cells: evidence for desferrioxamine as an inducer of oxidant stress in red blood cells.
BACKGROUND
Desferrioxamine (DFO) is an important iron chelating agent. It has also been thought of as an agent with anti-oxidant potential as it chelates ferric iron in various parts of the body. However, there is evidence suggesting that it may paradoxically affect red blood cells (RBC) by inducing intracellular oxidant stress. To further understand the mechanism of DFO's interaction with RBC, we conducted a study to determine the effect of DFO upon RBC's redox status.


METHODS
We examined NAD redox potential in intact RBC (N = 5) incubated with DFO. RBC were incubated with 6 mM DFO for 2 hours.


RESULTS
Significant decreases in NAD redox potential were observed after incubation of RBC with 6 mM DFO. The mean decrease was 10.01 PlusMinus; 1.98% (p < 0.0004).


CONCLUSIONS
The data confirm the oxidant effect of DFO on RBC.
Niihara
Yutaka
Y
Department of Medicine, Harbor-UCLA Medical Center, UCLA School of Medicine, Torrance, California, USA. ysniihara@msn.com
Ge
Junli
J
Shalev
Oded
O
Wu
Huiyuan
H
Tu
Amy
A
Tanaka
Kouichi R
KR
D003676:Deferoxamine / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007502:Iron Chelating Agents / Q000494:pharmacology*; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*
D007502:Iron Chelating Agents; D009243:NAD; D003676:Deferoxamine