Simultaneous detection of monohydroxybenzo[a]pyrene positional isomers by reversed-phase liquid chromatography coupled to electrospray ionization mass spectrometry.
A liquid chromatographic (LC) method has been developed for the separation of 11 monohydroxybenzo[a]pyrenes (OH BaPs) positional isomers, and for their detection using electrospray ionization mass spectrometry (ESI-MS). All OH BaPs isomers were separated on an octadecylsilyl (C(18))-bonded amorphous organosilica column utilizing gradient elution with acetonitrile-water and triethylamine (TEA) at pH 11.0 and determined by MS, except 2- and 8-OH BaPs which were coeluted. The lower detection limits were in the range from 1.6 micro g/L for 12-OH BaP to 12 micro g/L for 5-OH BaP without any sample enrichment. The relative standard deviations of area response were in the range from 1.8% (9-OH BaP) to 4.9% (12-OH BaP) except for 9.4% (7-OH BaP). The developed method was successfully applied to incubation mixtures of BaP and CYP1A1/epoxide hydrolase. This method identified 1-, 3- and 9-OH BaPs as the major metabolites, and 2- (and/or 8-) and 12-OH BaPs as the minor metabolites in the incubation mixture.
Sasaki
Hideki
H
Nihon Waters K.K., 3-12, Kitashingawa 1-chome, Shinagawa-ku, Tokyo 140-0001, Japan. hideki_sasaki@waters.com
Yonekubo
Jun
J
Kanai
Michiko
M
Toriba
Akira
A
Kizu
Ryoichi
R
Hayakawa
Kazuichi
K
D001564:Benzo(a)pyrene / Q000031:analogs & derivatives / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007536:Isomerism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D001564:Benzo(a)pyrene