cDNA cloning and expressions of cytochrome P450 1A in zebrafish embryos.
Cytochrome P450 1A (CYP1A) is well known for being induced by aromatic hydrocarbons, including 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD). We determined the complete cDNA sequence of a CYP1A open reading frame with both 5'- and 3'-ends in zebrafish (zfCYP1A), a useful model for environmental toxicology. zfCYP1A shows high percentage identity with CYP1As of mammals, domestic fowl and xenopus (51.9-60.4%), as well as the other fish species (63.8-89.2%). As revealed by in situ hybridization and immunohistochemistry, zfCYP1A was scarcely detected in control embryos but was markedly induced by TCDD especially in heart, vascular endothelial cells, intestinal epithelium, pronephros and outer integument in both prehatched and hatched embryos. These expression patterns are consistent with possible involvement of zfCYP1A in TCDD-induced toxicities.
Yamazaki
Koji
K
Department of Toxicology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Teraoka
Hiroki
H
Dong
Wu
W
Stegeman
John J
JJ
Hiraga
Takeo
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000201:enzymology* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*
D018076:DNA, Complementary; D019363:Cytochrome P-450 CYP1A1