Complementary use of partial least-squares and artificial neural networks for the non-linear spectrophotometric analysis of pharmaceutical samples.
The complementary use of partial least-squares (PLS) multivariate calibration and artificial neural networks (ANNs) for the simultaneous spectrophotometric determination of three active components in a pharmaceutical formulation has been explored. The presence of non-linearities caused by chemical interactions was confirmed by a recently discussed methodology based on Mallows augmented partial residual plots. Ternary mixtures of chlorpheniramine, naphazoline and dexamethasone in a matrix of excipients have been resolved by using PLS for the two major analytes (chlorpheniramine and naphazoline) and ANNs for the minor one (dexamethasone). Notwithstanding the large number of constituents, their high degree of spectral overlap and the occurrence of non-linearities, rapid and simultaneous analysis has been achieved, with reasonably good accuracy and precision. No extraction procedures using non-aqueous solvents are required.
Goicoechea
Héctor C
HC
Laboratorio de Control de Calidad de Medicamentos, Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe (3000), Argentina. hgoico@fbcb.unl.edu.ar
Collado
María S
MS
Satuf
María L
ML
Olivieri
Alejandro C
AC
D002744:Chlorpheniramine / Q000032:analysis; D003907:Dexamethasone / Q000032:analysis; D005938:Glucocorticoids / Q000032:analysis; D006634:Histamine H1 Antagonists / Q000032:analysis; D016018:Least-Squares Analysis; D009278:Naphazoline / Q000032:analysis; D014663:Nasal Decongestants / Q000032:analysis; D016571:Neural Networks, Computer; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D005938:Glucocorticoids; D006634:Histamine H1 Antagonists; D014663:Nasal Decongestants; D004364:Pharmaceutical Preparations; D002744:Chlorpheniramine; D003907:Dexamethasone; D009278:Naphazoline