Peptide fragments released from Phe-caseinomacropeptide in vivo in the rat.
The aim of this study was to investigate the pharmacokinetics of bovine Phe-caseinomacropeptide (Phe-CMP) in the rat after oral administration. This polypeptide was monophosphorylated and mainly nonglycosylated: Phe-CMP-1P. During gastrointestinal digestion and absorption, Phe-CMP-1P was degraded. Intact Phe-CMP-1P and CMP-1P were rapidly released from the stomach. In contrast, partial hydrolysis by pancreatic enzymes was observed. In vitro hydrolysis by brush-border membrane vesicles also indicated that the peptide was degraded. In the blood, "CMP-immunoreactive material" appeared rapidly, reaching a maximum level of 5.5 microg/ml at 60 min.
Fosset
S
S
Unité INRA/INAPG de Physiologie de la Nutrition et du Comportement Alimentaire, Institut National Agronomique de Paris-Grignon, 16 rue Claude Bernard, F-75231 Paris Cedex 05, France.
Fromentin
G
G
Gietzen
D W
DW
Dubarry
M
M
Huneau
J F
JF
Antoine
J M
JM
Lang
V
V
Mathieu-Casseron
F
F
Tomé
D
D
D000284:Administration, Oral; D000821:Animal Feed; D000818:Animals; D002364:Caseins / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics*; D002417:Cattle; D022162:Cytoplasmic Vesicles / Q000737:chemistry; D004063:Digestion; D005983:Glutens; D006868:Hydrolysis; D007408:Intestinal Absorption; D008297:Male; D010179:Pancreas / Q000201:enzymology; D010446:Peptide Fragments / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics*; D010649:Phenylalanine / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D002364:Caseins; D010446:Peptide Fragments; C065989:caseinomacropeptide; D010649:Phenylalanine; D005983:Glutens