Evaluation of the murine immune response to Leishmania meta 1 antigen delivered as recombinant protein or DNA vaccine.
The meta 1 gene of Leishmania is conserved across the genus and encodes a protein upregulated in metacyclic promastigotes. Meta 1 constitutive overexpressing mutants show increased virulence to mice. In this paper, both meta 1 recombinant protein and plasmids bearing the meta 1 gene were tested for their antigenicity and potential for inducing protective immunity in mice. Vaccination with the recombinant protein induced a predominant Th2-type of response and did not result in protection upon challenge with live parasites. Surprisingly, the expected reversal to a CD4(+) Th1-type of response upon genetic immunisation by the intramuscular route was not observed. Instead, vaccination with either the meta 1 gene alone or in fusion with the monocyte chemotactic protein (MCP)-3 cDNA induced a Th2-type of response that correlated with lack of protection against infection.
Serezani
Carlos Henrique Cardoso
CH
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av Prof Lineu Prestes, 1374, São Paulo, CEP 05508-900 Brazil.
Franco
Amanda Richards
AR
Wajc
Mariana
M
Umada Yokoyama-Yasunaka
Jenicer K
JK
Wunderlich
Gerhard
G
Borges
Monamaris Marques
MM
Uliana
Silvia Reni Bortolin
SR
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D019556:COS Cells / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D054410:Chemokine CCL7; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D018320:Leishmania major / Q000276:immunology*; D007894:Leishmania mexicana / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000517:prevention & control; D016774:Leishmaniasis, Diffuse Cutaneous / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D018945:Monocyte Chemoattractant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D015800:Protozoan Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D016052:Protozoan Vaccines / Q000008:administration & dosage* / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D012995:Solubility; D016176:T-Lymphocyte Subsets / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology / Q000627:therapeutic use; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
D000913:Antibodies, Protozoan; C515451:Ccl7 protein, mouse; D054410:Chemokine CCL7; D016207:Cytokines; C100528:Meta1 protein, Leishmania major; D018945:Monocyte Chemoattractant Proteins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic