Characterization of Clostridium butyricum neurotoxin associated with food-borne botulism.
The neurotoxin of Clostridium butyricum strain LCL155 (BuNT/LCL155) associated with type E food-borne botulism showed antigenic and biological properties different from those of C. botulinum type E (BoNT/E) andC. butyricum strain BL5262 (BuNT/BL5262). The specific toxicity of BuNT/LCL155 was found to be about 10% those of BoNT/E and BuNT/BL5262. Immunological analysis with monoclonal antibodies against BoNT/E showed that the heavy chain of BuNT/LCL155 differs partially from those of BoNT/E and BuNT/BL5262. Binding experiments with rat brain synaptic membrane revealed that BuNT/LCL155 possesses a binding activity lower than either BoNT/E or BuNT/BL5262. There was no difference in the catalytic activity of the three neurotoxins, which had been determined with a recombinant of the intracellular target protein SNAP-25. These data suggest that the BuNT/LCL155 shares the receptor-recognition site structurally different from BoNT/E and BuNT/BL5262, perhaps causing a decreased specific toxicity.
Tsukamoto
Kentaro
K
Department of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
Mukamoto
Masafumi
M
Kohda
Tomoko
T
Ihara
Hideshi
H
Wang
Xingmin
X
Maegawa
Tsuneo
T
Nakamura
Shinichi
S
Karasawa
Tadahiro
T
Kozaki
Shunji
S
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001906:Botulism / Q000382:microbiology*; D003013:Clostridium / Q000472:pathogenicity*; D004797:Enzyme-Linked Immunosorbent Assay; D005516:Food Microbiology; D005732:Gangliosides / Q000378:metabolism; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry / Q000276:immunology / Q000633:toxicity*; D051381:Rats
D005732:Gangliosides; D009498:Neurotoxins