Pleurotus Eous mushroom lectin (PEL) with mixed carbohydrate inhibition and antiproliferative activity on tumor cell lines.
A novel Pleurotus eous mushroom lectin (PEL) having mixed binding specificity was purified to homogeneity from the fruitbodies of P. eous. The lectin showed monomer weight of 16 kDa and was a glycoprotein having a 9% neutral sugar content. The antiproliferative activity of PEL was tested on four human tumor cell lines. A 50% inhibition of proliferation was demonstrated in MCF-7, K562 and Hep2 cell lines at the lowest concentration (2 microg/ml) of lectin tested. SK-N-MC cell line showed 50% inhibition at 50 microg/ml of PEL. The inhibitory activity of PEL was not associated with toxicity to cell lines.
Mahajan
R G
RG
Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, India. padmashastry@hotmail.com
Patil
Satyali I
SI
Mohan
D R K
DR
Shastry
Padma
P
D000970:Antineoplastic Agents / Q000633:toxicity*; D002294:Carcinoma, Squamous Cell; D002455:Cell Division / Q000187:drug effects; D002850:Chromatography, Gel; D006595:Hexosamines / Q000494:pharmacology; D006601:Hexoses / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D007822:Laryngeal Neoplasms; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D020076:Pleurotus / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D006595:Hexosamines; D006601:Hexoses; D037102:Lectins