Small molecule affinity fingerprinting. A tool for enzyme family subclassification, target identification, and inhibitor design.
Classifying proteins into functionally distinct families based only on primary sequence information remains a difficult task. We describe here a method to generate a large data set of small molecule affinity fingerprints for a group of closely related enzymes, the papain family of cysteine proteases. Binding data was generated for a library of inhibitors based on the ability of each compound to block active-site labeling of the target proteases by a covalent activity based probe (ABP). Clustering algorithms were used to automatically classify a reference group of proteases into subfamilies based on their small molecule affinity fingerprints. This approach was also used to identify cysteine protease targets modified by the ABP in complex proteomes by direct comparison of target affinity fingerprints with those of the reference library of proteases. Finally, experimental data were used to guide the development of a computational method that predicts small molecule inhibitors based on reported crystal structures. This method could ultimately be used with large enzyme families to aid in the design of selective inhibitors of targets based on limited structural/function information.
Greenbaum
Doron C
DC
Department of Pharmaceutical Chemistry, San Francisco, CA 94143, USA.
Arnold
William D
WD
Lu
Felice
F
Hayrapetian
Linda
L
Baruch
Amos
A
Krumrine
Jennifer
J
Toba
Samuel
S
Chehade
Kareem
K
Brömme
Dieter
D
Kuntz
Irwin D
ID
Bogyo
Matthew
M
D000465:Algorithms; D000818:Animals; D001665:Binding Sites; D016000:Cluster Analysis; D015853:Cysteine Proteinase Inhibitors / Q000737:chemistry* / Q000378:metabolism; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004852:Epoxy Compounds / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D010206:Papain / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D016415:Sequence Alignment; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D015853:Cysteine Proteinase Inhibitors; D004852:Epoxy Compounds; D010206:Papain