[Establishment of biopanning model of phage display peptide library in the blood vessels of excised human osteosarcoma vasculature and its significance].
OBJECTIVE
To establish an in vivo biopanning model of phage display peptide library in the blood vessels contained in surgically removed human osteosarcoma.


METHODS
In 28 patients with osteosarcoma, digital subtraction angiography (DSA) of the involved limb was performed preoperatively to understand the approximate status of the arteries in the tumors. The tumors were then surgically removed and carefully trimmed, perfused via a simulated Langendorff perfusion apparatus with the indexes as the pH value, temperature and O2 partial pressure monitored in the blood vessels. A 12-meres phage display peptide library was biopanned to isolate peptides capable of homing specifically to the excised osteosarcoma.


RESULTS
In vivo biopanning models were successfully established in all the excised tumors, which could be directly used in perfusion experiment and the indexes monitored in the blood vessels in the tumors were comparable to those of living tissues. Some high-affinity peptides specific to the blood vessels in osteosarcoma were obtained with the motif of RLTR.


CONCLUSIONS
In vivo biopanning model simulating the Langendorff perfusion apparatus can be easily established which is instrumental for the application of phage display technology in human living tissues and may facilitate the study of targeted chemotherapy of osteosarcoma.
Li
Zhong-Qi
ZQ
Department of Orthopedics, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. zongqil@263.net
Shi
Zhan-Jun
ZJ
Hao
Wen-Bo
WB
L
Hai
H
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics; D001667:Binding, Competitive; D001808:Blood Vessels / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D001831:Body Temperature; D002648:Child; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008954:Models, Biological; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use; D012516:Osteosarcoma / Q000098:blood supply* / Q000235:genetics; D010100:Oxygen / Q000097:blood; D010313:Partial Pressure; D019151:Peptide Library; D010477:Perfusion
D009842:Oligopeptides; D019151:Peptide Library; D010100:Oxygen