Genetic mapping and tagging of wheat genes using RAPD, STS and SSR markers.
We applied SSR markers for mapping genes determining red coleoptile colour in wheat (Rc1, Rc2, Rc3) using F2 populations. All three genes map at about 15 to 20 cM distally from the centromere of chromosomes 7AS, 7BS and 7DS, respectively. The locations of the glume colour (Bg, Rg1) and glume hairiness (Hg) genes relative to the SSR markers of the homoeologous chromosomes group 1 were determined using molecular analysis of near-isogenic lines (NILs). One RAPD marker for the vernalisation response gene Vrn-A1 was identified by screening 95 random primers against two pairs of NILs. New PCR (STS) markers were developed based on RFLP-markers PSR426 (5A, 5B, 5D) and PSR1201 (1A, 5A, 5B). Analysis of nulli-tetrasomic and near-isogenic lines of wheat using the STS markers developed gave an indication that these new STS markers have the same chromosomal and intrachromosomal positions as the correspondent RFLP markers. Therefore, they could be used for mapping and/or tagging the vernalisation response (Vrn-A1, Vrn-B1, Vrn-D1) and homoeologous pairing (Ph1) genes.
Khlestkina
Elena K
EK
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. khlest@bionet.nsc.ru
Pestsova
Elena G
EG
Salina
Elena
E
Röder
Marion S
MS
Arbuzova
Valentina S
VS
Koval
Sergej F
SF
Börner
Andreas
A
D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D005819:Genetic Markers; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique; D016324:Sequence Tagged Sites; D014908:Triticum / Q000235:genetics*
D018744:DNA, Plant; D005819:Genetic Markers