Development of a RT-nested PCR test detecting pigeon Paramyxovirus-1 directly from organs of infected animals.
A RT-nested PCR that amplifies part of the conserved nucleoprotein gene of avian Paramyxovirus type 1 is described. The technique allowed the detection of pigeon Paramyxovirus type 1 (pPMV-1) virus directly from a wide range of infected chicken and pigeon organs, and should be able to detect typical Newcastle disease viruses too. Compared with the reference method, the developed RT-nested PCR was found more sensitive, as it was able to detect virus genome in infected pigeon organs at late stage of infection, when virus isolation failed. Such a molecular technique represents an alternative method of diagnosis for research purposes on pPMV-1 variants, for example to study pathogenesis aspects of the infection or to assess the efficacy of vaccines.
Barbezange
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AFSSA-site de Ploufragan, U VIPAC, BP53, 22440 Ploufragan, France.
Jestin
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D000818:Animals; D001715:Bird Diseases / Q000821:virology; D002645:Chickens; D010856:Columbidae; D017931:DNA Primers; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000302:isolation & purification*; D009928:Organ Specificity; D011201:Poultry Diseases / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers