Absolute concentrations of high-energy phosphate metabolites in normal, hypertrophied, and failing human myocardium measured noninvasively with (31)P-SLOOP magnetic resonance spectroscopy.
OBJECTIVE
The purpose of the present study was to measure absolute concentrations of phosphocreatine (PCr) and adenosine triphosphate (ATP) in normal, hypertrophied, and failing human heart.


BACKGROUND
Conflicting evidence exists on the extent of changes of high-energy phosphate metabolites in hypertrophied and failing human heart. Previous reports using phosphorus-31 magnetic resonance spectroscopy ((31)P-MRS) have quantified metabolites in relative terms only. However, this analysis cannot detect simultaneous reductions.


METHODS
Four groups of subjects (n = 10 each), were studied: volunteers and patients with hypertensive heart disease (HHD), aortic stenosis, and dilated cardiomyopathy (DCM). Left ventricular (LV) function and mass were measured by cine magnetic resonance imaging. Absolute and relative concentrations of PCr and ATP were determined by (31)P-MRS with spatial localization with optimum point spread function.


RESULTS
Left ventricular ejection fraction remained normal in HHD and aortic stenosis, but was severely reduced to 18% in DCM; LV mass was increased by 55%, 79%, and 68% respectively. In volunteers, PCr and ATP concentrations were 8.82 +/- 1.30 mmol/kg wet weight and 5.69 +/- 1.02 mmol/kg wet weight, and the PCr/ATP ratio was 1.59 +/- 0.33. High-energy phosphate levels were unaltered in HHD. In aortic stenosis, PCr was decreased by 28%, whereas ATP remained constant. In DCM, PCr was reduced by 51%, ATP by 35%, and reduction of the PCr/ATP ratio by 25% was of borderline significance (p = 0.06). Significant correlations were observed among energetic and functional variables, with the closest relations for PCr.


CONCLUSIONS
In human heart failure due to DCM, both PCr and ATP are significantly reduced. Ratios of PCr to ATP underestimate changes of high-energy phosphate levels.
Beer
Meinrad
M
Institut für Röntgendiagnostik, Würzburg, Germany. meinrad.beer@mail.uni-wuerzburg.de
Seyfarth
Tobias
T
Sandstede
Jörn
J
Landschütz
Wilfried
W
Lipke
Claudia
C
Köstler
Herbert
H
von Kienlin
Markus
M
Harre
Kerstin
K
Hahn
Dietbert
D
Neubauer
Stefan
S
D000255:Adenosine Triphosphate / Q000032:analysis*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001024:Aortic Valve Stenosis / Q000175:diagnosis* / Q000378:metabolism* / Q000503:physiopathology; D015982:Bias; D002311:Cardiomyopathy, Dilated / Q000175:diagnosis* / Q000378:metabolism* / Q000503:physiopathology; D016022:Case-Control Studies; D004734:Energy Metabolism; D005260:Female; D006801:Humans; D006973:Hypertension / Q000150:complications*; D017379:Hypertrophy, Left Ventricular / Q000175:diagnosis* / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000737:chemistry* / Q000378:metabolism; D010725:Phosphocreatine / Q000032:analysis*; D010759:Phosphorus Isotopes; D013318:Stroke Volume; D016277:Ventricular Function, Left
D010759:Phosphorus Isotopes; D010725:Phosphocreatine; D000255:Adenosine Triphosphate