The effects of vitamin E succinate on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells.
OBJECTIVE
To investigate the effects of vitamin E succinate (VES) on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells.


METHODS
After SGC-7901 cells were treated with VES at different doses (5,10,20 mg x L(-1)) at different time, reverse transcription-PCR technique was used to detect the level of c-jun mRNA; Western Blot was applied to measure the expression of c-jun protein.


RESULTS
After the cells were treated with VES at 20 mg x L(-1) for 3 h, the expression rapidly reached its maximum that was 3.5 times of UT control (P<0.01). The level of c-jun mRNA was also increased following treatment of VES for 6 h. However,the expression after treatment of VES at 5 mg x L(-1) for 24 h was 1.6 times compared with UT control (P<0.01). Western blot analysis showed that the level of c-jun protein was obviously elevated in VES-treated SGC-7901 cells at 20 mg x L(-1) for 3 h. The expression of c-jun protein was gradually increased after treatment of VES at 20 mg x L(-1) for 3, 6, 12 and 24 h, respectively, with an evident time-effect relationship.


CONCLUSIONS
The levels of c-jun mRNA and protein in VES-treated SGC-7901 cells were increased in a dose- and time-dependent manner; the expression of c-jun was prolonged by VES, indicating that c-jun is involved in VES-induced apoptosis in SGC-7901 cells.
Zhao
Yan
Y
Department of Nutrition and Food Hygiene, Public Health School, Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
Wu
Kun
K
Xia
Wei
W
Shan
Yu-Juan
YJ
Wu
Li-Jie
LJ
Yu
Wei-Ping
WP
D015153:Blotting, Western; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D013274:Stomach Neoplasms; D024505:Tocopherols; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology*
D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D014810:Vitamin E; D024505:Tocopherols