title: Histochemical localisation of FMRFamide-gated Na+ channels in Helisoma trivolvis and Helix aspersa neurones.
abstract: FMRFamide-gated Na+ channels of molluscan neurones belong to the ENa/Deg family of channels which have diverse functions. FMRFamide (Phe-Met-Arg-Phe-NH2) Na+ channels were detected electrophysiologically in specified neurones of Helix (Helix aspersa) and Helisoma (Helisoma trivolvis), and clones (FaNaCs) subsequently identified. We have now made a study to determine the distribution of mRNA for the clones HaFaNaC (Helix) and HtFaNaC (Helisoma) in the nervous systems of these species using standard in situ hybridization techniques. Immunohistochemical experiments were also made using an HtFaNaC antibody to detect the channel protein in Helisoma neurones. Many neurones in the central ganglia, including those which exhibit the FMRFamide Na+ current, stained for FaNaC-mRNA, suggesting a much wider distribution of the channel than was indicated by the earlier work. An immunoreactive response to the channel antibody was also observed in some Helisoma neurones, such as the giant dopamine neurone of the left pedal ganglion, also shown to possess HtFaNaC-mRNA and to exhibit the FMRFamide Na+ current. Taken together, these experiments suggest that the clones HaFaNaC and HtFaNaC are major, if not the only, subunits of the FMRFamide-gated Na+ channel detected electrophysiologically in the identified neurones of these species. However, fewer neurones in Helisoma reacted with the HtFaNaC-antibody than those which exhibited message for the channel. This discrepancy may be due to a difference in sensitivity of the two techniques, or because not all of the channel mRNA is normally expressed as a membrane protein.
authors.affiliation: School of Biology, University of St Andrews, St Andrews, Fife, KY16 9TS, UK. gac@st-andrews.ac.uk
authors.forename: F
authors.initials: F
authors.lastname: Davey
authors.forename: S J
authors.initials: SJ
authors.lastname: Harris
authors.forename: G A
authors.initials: GA
authors.lastname: Cottrell
mesh_terms: D000818:Animals; D004298:Dopamine / Q000032:analysis; D019835:FMRFamide / Q000494:pharmacology*; D019084:Fluorescent Antibody Technique, Indirect; D017952:Ganglia, Invertebrate / Q000166:cytology; D015870:Gene Expression; D006372:Helix, Snails / Q000166:cytology; D017403:In Situ Hybridization; D015640:Ion Channel Gating / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000187:drug effects / Q000235:genetics; D009474:Neurons / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000032:analysis; D012680:Sensitivity and Specificity; D012908:Snails / Q000166:cytology*; D015222:Sodium Channels / Q000032:analysis* / Q000187:drug effects / Q000235:genetics
chemical_list: C097095:FaNaCh protein, Helix aspersa; D009419:Nerve Tissue Proteins; D016372:RNA, Antisense; D012333:RNA, Messenger; D015222:Sodium Channels; D019835:FMRFamide; D004298:Dopamine