title: Combined one-tube osmotic fragility (OF) test and dichlorophenol-indolphenol (DCIP) test screening for hemoglobin disorders, an experience in 213 Thai pregnant women.
abstract: OBJECTIVE
  Hemoglobin disorders are an important health problem in Thailand. Therefore, screening tests for abnormal genotypes offer an effective method for coping with this problem. This study has been designed to study basic screening tests for inherited hemoglobin disorders in Thai pregnant women.
  
  
  METHODS
  This study was designed as a diagnostic test. EDTA blood specimens from 213 pregnant women were used in this study. The screening methods studied were the one-tube osmotic fragility (OF) test and the dichlorophenol-indolphenol (DCIP) test. The standard method used in this study was hemoglobin typing applying the cellulose acetate hemoglobin electrophoresis method. Diagnostic properties such as sensitivity, specificity and predictive value were used for data analysis.
  
  
  RESULTS
  The results obtained from this study demonstrated the sensitivity, specificity, positive and negative predictive value for Hb E disorders screened by the DCIP test to be 100%, 97.2%, 94.4% and 100%, respectively. For beta thalassemia disorder screened by the OF test, the sensitivity, specificity, positive and negative predictive value were 100%, 83.7%, 10.5% and 100%, respectively. Evaluation for combining both methods as screening tools for all hemoglobin disorders revealed a sensitivity, specificity, positive and negative predictive value of 100%, 97.1%, 94.9% and 100%, respectively.
  
  
  CONCLUSIONS
  The combination of both the OF and the DCIP test as a screening tool for hemoglobin disorders in pregnant women is an effective method and should be used in the antenatal clinic.
authors.affiliation: Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. Viroj.W@Chula.ac.th
authors.forename: V
authors.initials: V
authors.lastname: Wiwanitkit
authors.forename: J
authors.initials: J
authors.lastname: Suwansaksri
authors.forename: N
authors.initials: N
authors.lastname: Paritpokee
mesh_terms: D015086:2,6-Dichloroindophenol; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D006403:Hematologic Tests; D006446:Hemoglobin E / Q000032:analysis; D006453:Hemoglobinopathies / Q000175:diagnosis*; D006455:Hemoglobins, Abnormal / Q000032:analysis; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D009996:Osmotic Fragility; D011237:Predictive Value of Tests; D011247:Pregnancy; D012680:Sensitivity and Specificity; D013785:Thailand / Q000453:epidemiology; D017086:beta-Thalassemia / Q000175:diagnosis
chemical_list: D006455:Hemoglobins, Abnormal; D006446:Hemoglobin E; D015086:2,6-Dichloroindophenol