Formation of active oxygen species from diethylstilbestrol, a synthetic estrogen, and its metabolite in the presence of RAW 264.7 cells.
Previously, we reported that (+/-)-IA but not DES produces O2- spontaneously in PBS. We are interested in the possibility that these compounds might produce active oxygen species under mild cell culture conditions. On incubation of RAW 264.7 cells with (+/-)-IA, the signal of 5,5'-dimethyl-1-pyrroline-N-oxide (DMPO)-OH adducts increased but no more than the additive effect. However, stimulation of RAW cells with LPS and INF-gamma enhanced the formation of DMPO-OH adducts slightly more than the additive effect, especially when the concentration of (+/-)-IA increased. In the case of DES, the spectra of DMPO-OH adducts did not increase concentration-dependently in the absence of RAW 264.7 cells, however in their presence, they increased concentration-dependently, especially when these cells were stimulated with LPS and IFN-gamma. The results were interpreted to mean that DES would have a higher oxidation potential than (+/-)-IA, not be oxidized to semiquinoes spontaneously, and therefore not produce DMPO-OH adducts in the absence of RAW cells. In their presence, DES might be easily oxidized to semiquinones by the reaction with O2- produced from RAW 264.7 cells.
Oda
Taiko
T
Department of Biochemistry, Kyoritsu College of Pharmacy, Tokyo, Japan. oda-ti@kyoritsu-ph.ac.jp
Kaneko
Motohisa
M
D000818:Animals; D002460:Cell Line; D004054:Diethylstilbestrol / Q000737:chemistry / Q000378:metabolism*; D045166:Estradiol Congeners / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism*
D045166:Estradiol Congeners; D017382:Reactive Oxygen Species; D004054:Diethylstilbestrol