title: Interleukin-1beta expression on ovarian granulosa cells and its clinical implication in women undergoing in vitro fertilization.
abstract: OBJECTIVE
  To detect the expression of interleukin-1beta (IL-1beta) on the surface of human luteinized granulosa cells and to understand its relation to the hormones in human follicular fluid and sera.
  
  
  METHODS
  Thirty-six women receiving in vitro fertilization-embryo transfer (IVF-ET) were enrolled in this study. The luteinizing granulosa cells were recovered from the follicular fluid obtained during transvaginal ultrasound-guided oocyte retrieval for IVF-ET. Flow cytometry was employed to detect the expression of IL-1beta on human granulosa cells, and the concentrations of IL-1beta and the hormones in the follicular fluid were measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  IL-1beta was detected in the follicular fluid of women with IVT-ET and in the luteinizing granulosa cells. A positive correlation was observed between the sera concentrations of IL-1beta and estradiol on the day when human chorionic gonadotrophin (hCG) was injected (r=0.92, P<0.001), while on the same day the concentration of IL-1beta was shown to be inversely correlated with progesterone levels (r=-0.34, P<0.05).
  
  
  CONCLUSIONS
  IL-1beta in the follicular fluid comes not only from plasma ultrafiltrate but also from luteinizing granulosa cells. The inhibition of progesterone production by luteal cells in the presence of IL-1beta may be one of the factors resulting in the failure of implantation.
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Wang
authors.forename: Fu-Qi
authors.initials: FQ
authors.lastname: Xing
authors.forename: Shi-Ling
authors.initials: SL
authors.lastname: Chen
mesh_terms: D000328:Adult; D015232:Dinoprostone / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000097:blood; D006107:Granulosa Cells / Q000276:immunology*; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000276:immunology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D013373:Substance P / Q000097:blood
chemical_list: D007375:Interleukin-1; D013373:Substance P; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D015232:Dinoprostone