Methodology of platelet membrane glycoproteins detection in whole blood by flow cytometry.
OBJECTIVE
To establish a method for platelet membrane glycoproteins (PMGP) detection in the whole blood by flow cytometry (FCM) and to evaluate the methodological factors involved.


METHODS
Expressions of PMGP GP I b-I X, GP II b-III a and P-selectin were detected by FCM under different experimental conditions to evaluate the factors affecting their expressions.


RESULTS
FCM rapidly and accurately detected PMGP expressions with distinct specificity and sufficient sensitivity, and reduced the chances for artificial activation of the platelet during the experiment.


CONCLUSIONS
FCM provides a new method for PMGP function studies.
Chen
Lian-Bo
LB
Central Laboratory, First Military Medical University, Guangzhou 510515, China.
Dong
Wei-Ren
WR
Dong
Fang
F
D000328:Adult; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006490:Hemostatics / Q000494:pharmacology; D006801:Humans; D008297:Male; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis*; D013917:Thrombin / Q000494:pharmacology
D006490:Hemostatics; D010980:Platelet Membrane Glycoproteins; D013917:Thrombin