Capillary electrophoresis assay of alanine:glyoxylate aminotransferase activity in rat liver.
We measured hepatic alanine:glyoxylate aminotransferase (AGT) activity using capillary electrophoresis. After rat liver homogenate was incubated in the presence of substrates and pyridoxal 5'-phosphate, the pyruvate and glycine produced by AGT were measured. The AGT activity was 10.02+/-0.31 micro mol pyruvate/h/mg protein and 10.21+/-0.15 micro mol glycine/h/mg protein. This method is relatively simple and shows superior sensitivity, allowing the measurement of enzyme activity in 5 micro g of protein. Therefore, it appears to be suitable for laboratory use and may also have advantages for measuring AGT activity in liver biopsy specimens.
Nishijima
Saori
S
Department of Urology, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Okinawa Nishihara 903-0215, Japan. k998739@med.u-ryukyu.ac.jp
Sugaya
Kimio
K
Morozumi
Makoto
M
Hatano
Tadashi
T
Ogawa
Yoshihide
Y
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D000637:Transaminases / Q000378:metabolism*
D000637:Transaminases; C019635:Alanine-glyoxylate transaminase