[In vivo HSV-TK gene transfer and the therapeutic effects of ganciclovir on bladder cancer in mice with HSV-TK gene transfer].
OBJECTIVE
To explore an effective method for in vivo HSV-TK gene transfer and observe the therapeutic effect of ganciclovir (GCV) on bladder cancer in mice after receiving such gene transfer therapy.


METHODS
Mice models bearing subcutaneous tumors of bladder cancer were established in 24 syngeneic mice, and HSV-TK gene transfer was performed by means of naked DNA plasmids, DNA-liposome complex or retroviral mediators respectively. GCV treatment was administered in all the mice and its effect on the growth of the tumor was subsequently observed.


RESULTS
Gene transfer therapies by DNA-liposome complex and retroviral mediators both significantly inhibited the growth of the tumor by 54% and 68% respectively, and the survival of the mice was prolonged by 28.81% and 44.16% (P<0.05). The effect of gene transfer by naked DNA plasmids was not obvious (P>0.05).


CONCLUSIONS
Gene transfer therapies by DNA-liposome complex and retroviral vectors supplemented by GCV treatment are effective therapeutic modalities against bladder cancer.
Liu
Wei-Chi
WC
Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Ye
Gang
G
Zhang
Rong-Gui
RG
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D015774:Ganciclovir / Q000378:metabolism / Q000627:therapeutic use*; D018014:Gene Transfer Techniques; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011355:Prodrugs / Q000627:therapeutic use*; D018139:Simplexvirus / Q000201:enzymology; D013937:Thymidine Kinase / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy*
D000970:Antineoplastic Agents; D011355:Prodrugs; D013937:Thymidine Kinase; D015774:Ganciclovir