Comparative proteomic analysis of extracellular proteins of Edwardsiella tarda.
A comparison of extracellular proteins of virulent and avirulent Edwardsiella tarda strains revealed several major, virulent-strain-specific proteins. Proteomic analysis identified two of the proteins in the virulent strain PPD130/91 as flagellin and SseB, which are virulence factors in bacterial pathogens. PCR amplification and DNA sequencing confirmed the presence of the genes that encode these proteins. Our results clearly demonstrated the potency of the proteomic approach in identifying virulence factors.
Tan
Y P
YP
Department of Biological Sciences, Faculty of Science. Tropical Marine Science Institute, the National University of Singapore, Singapore 117543.
Lin
Q
Q
Wang
X H
XH
Joshi
S
S
Hew
C L
CL
Leung
K Y
KY
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D020609:Edwardsiella tarda / Q000737:chemistry / Q000235:genetics / Q000472:pathogenicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D029968:Escherichia coli Proteins; D005408:Flagellin / Q000032:analysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D040901:Proteomics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014774:Virulence
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C099931:SseB protein, E coli; D005408:Flagellin