Effects of dietary deficiency of selective amino acids on the function of the cornea and lens in rats.
Effects of dietary deficiencies of tryptophan and methionin on the transparency of cornea and lens were investigated in young rats (Brown-Norway, BN; Sprague-Dawley, SD) over 3 months. Transparency of the cornea and lens were evaluated in weekly intervals using a photo-slitlamp microscope. After sacrifice and lens fresh weight determination the lenses were prepared for histopathology. Methionin deficiency had no effect on the parameters investigated. Tryptophan deficiency caused severe loss of body weight in both strains, with additional loss of hair in SD rats. These developed corneal neovascularisations and cataracts. BN rats showed an enhanced zone of discontinuity in the lens. Diet intermission arrested the pathological processes in the eye which restarted when feeding the diet again. This observation is supported by lens fresh weight data. DNA staining evidenced that tryptophan deficiency arrested lens fiber maturation in both strains but stimulated corneal neovascularisation only in SD rats.
Wegener
A
A
Department of Experimental Ophthalmology, Medical Faculty, Rheinische Friedrich-Wilhelms-Universität, Bonn, Germany. a.wegener@uni-bonn.de
Golubnitschaja
O
O
Breipohl
W
W
Schild
H H
HH
Vrensen
G F J M
GF
D000818:Animals; D003315:Cornea / Q000033:anatomy & histology / Q000378:metabolism*; D004032:Diet; D005260:Female; D007908:Lens, Crystalline / Q000033:anatomy & histology / Q000378:metabolism*; D008715:Methionine / Q000172:deficiency*; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains; D017207:Rats, Sprague-Dawley; D014364:Tryptophan / Q000172:deficiency*
D014364:Tryptophan; D008715:Methionine