Interstitial PO(2) determination by phosphorescence quenching microscopy.
OBJECTIVE
This study introduces the technique of microinjection of phosphor probe into skeletal muscle tissue to determine oxygen tension (PO(2)) in the interstitium by phosphorescence quenching microscopy.


METHODS
The spinotrapezius muscle of Wistar-Kyoto rats weighing 240-280 g was surgically isolated and underwent the microinjection procedure. We measured the spatial distribution of phosphor probe 10, 30, and 80 minutes after injection; the tissue PO(2) at sites adjacent to arteriolar and venular microvessels; and the decline in tissue PO(2) during a 3-minute period of 8-Hz contraction.


RESULTS
The phosphorescence signal from the probe was undetectable outside a 2.5-mm radius from the site of injection at the 10-minute time point, increased to measurable values after 30 minutes, and was double the 30-minute intensity value after 80 minutes. When used to measure periarteriolar PO(2), the tissue microinjection technique demonstrated a nonlinear fall in tissue PO(2) with distance away from secondary arterioles (30-40 microm diameter). Conversely, perivenular tissue PO(2) increased in a nonlinear manner with distance away from secondary venules (60-70 microm diameter). The tissue PO(2) at distances of 16 microm and greater from both types of secondary microvessels was significantly different from values taken directly over the centerline of these microvessels. During muscle contraction, the PO(2) fell from a mean precontraction value of 28.3 +/- 4.9 mmHg to 8.2 +/- 0.9 mmHg at the end of the contraction period.


CONCLUSIONS
These observations indicate that the microinjection technique yields values for tissue PO(2) that are in good agreement with previously published results using oxygen microelectrodes.
Smith
Lane M
LM
Department of Physiology, Virginia Commonwealth University/Medical College of Virginia Campus, Richmond, VA 23298-0551, USA.
Golub
Aleksander S
AS
Pittman
Roland N
RN
D000818:Animals; D005110:Extracellular Space / Q000737:chemistry*; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008297:Male; D008722:Methods; D008833:Microcirculation; D008845:Microinjections; D008853:Microscopy / Q000379:methods*; D015335:Molecular Probes / Q000008:administration & dosage; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000098:blood supply / Q000737:chemistry / Q000166:cytology; D010100:Oxygen / Q000032:analysis*; D010313:Partial Pressure; D051381:Rats; D017208:Rats, Wistar
D015335:Molecular Probes; D010100:Oxygen