Ethanol impairs monosaccharide uptake and glycosylation in cultured rat astrocytes.
Astrocyte and glial-neuron interactions have a critical role in brain development, which is partially mediated by glycoproteins, including adhesion molecules and growth factors. Ethanol affects the synthesis, intracellular transport, subcellular distribution and secretion of these glycoproteins, suggesting alterations in glycosylation. We analyzed the effect of long-term exposure to low doses of ethanol (30 mm) on glycosylation process in growing cultured astrocytes in vitro. Cells were incubated for short (5 min) and long (90 min) periods with several radioactively labeled carbohydrate precursors. The uptake, kinetics and metabolism of these precursors, as well as the radioactivity distribution in protein gels were analyzed. The levels of GLUT1 and mannosidase II were also determined. Ethanol increased the uptake of monosaccharides and the protein levels of GLUT1 but decreased those of mannosidase II. It altered the carbohydrate moiety of proteins and increased cell surface glycoproteins containing terminal non-reduced mannose. These results indicate that ethanol impairs glycosylation in rat astrocytes, thus disrupting brain development.
Tomás
M
M
Centro de Investigación, Department of Biology and Cellular Pathology, Hospital Universitario La Fe, Avda. Campenar 21, E-46009 Valencia, Spain.
Fornas
E
E
Megías
L
L
Durán
J-M
JM
Portolés
M
M
Guerri
C
C
Egea
G
G
Renau-Piqueras
J
J
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002455:Cell Division; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000494:pharmacology*; D051272:Glucose Transporter Type 1; D006031:Glycosylation / Q000187:drug effects; D007150:Immunohistochemistry; D037102:Lectins / Q000737:chemistry / Q000633:toxicity; D008358:Mannose / Q000737:chemistry; D008361:Mannosidases / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009005:Monosaccharides / Q000378:metabolism* / Q000493:pharmacokinetics; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D051381:Rats; D013997:Time Factors
D051272:Glucose Transporter Type 1; D037102:Lectins; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D009005:Monosaccharides; C495679:Slc2a1 protein, rat; D000431:Ethanol; D008361:Mannosidases; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase; D019158:N-Acetylneuraminic Acid; D008358:Mannose