title: The expression of the dodecameric ferritin in Listeria spp. is induced by iron limitation and stationary growth phase.
abstract: The Gram-positive bacterium Listeria innocua possesses an authentic ferritin with an unusual dodecameric assemblage that resembles the quaternary structure of the DNA-binding proteins designated Dps (DNA-binding proteins from starved cells). The L. innocua gene encoding the above protein, termed ferritin from Listeria innocua (fri), has been localized on a 3-kb HindIII chromosomal fragment cloned in the Escherichia coli strain DH5alphaF'. DNA sequence analysis reveals an open reading frame of 468 nucleotides matching perfectly the amino acid sequence of the protein. Primer extension analysis indicates the presence of two transcriptional startpoints located 36 (proximal) and 85 nt (distal) upstream the fri start codon, respectively. Each transcriptional startpoint is preceded by suitably located -10 and -35 elements, which match the sigma(A) (proximal) and sigma(B) (distal) consensus sequences.In L. innocua and Liseria monocytogenes, fri expression increases both upon entry into stationary phase and, more markedly, under low-iron growth conditions. The effect of iron is apparent in the exponential and stationary phases of growth. An up-regulation by iron limitation has never been observed in other proven ferritins and bacterioferritins, but has been reported for several members of the Dps family. The unusual regulation by iron of the Listeria ferritin gene provides further support to the evolutionary link with the Dps family and suggests that the iron storage function may not be the unique role of ferritin in the physiology of this bacterium.
authors.affiliation: Dipartimento di Scienze di Sanità Pubblica, University of Rome La Sapienza, P.le A. Moro, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Polidoro
authors.forename: Daniela
authors.initials: D
authors.lastname: De Biase
authors.forename: Benedetta
authors.initials: B
authors.lastname: Montagnini
authors.forename: Laura
authors.initials: L
authors.lastname: Guarrera
authors.forename: Stefano
authors.initials: S
authors.lastname: Cavallo
authors.forename: Piera
authors.initials: P
authors.lastname: Valenti
authors.forename: Simonetta
authors.initials: S
authors.lastname: Stefanini
authors.forename: Emilia
authors.initials: E
authors.lastname: Chiancone
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D019281:Dimerization; D005293:Ferritins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000378:metabolism / Q000494:pharmacology*; D008087:Listeria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005293:Ferritins; D007501:Iron