Growth and differentiation of mouse tracheal epithelial cells: selection of a proliferative population.
Highly regulated programs for airway epithelial cell proliferation and differentiation during development and repair are often disrupted in disease. These processes have been studied in mouse models; however, it is difficult to isolate and identify epithelial cell-specific responses in vivo. To investigate these processes in vitro, we characterized a model for primary culture of mouse tracheal epithelial cells. Small numbers of cells seeded at low density (7.5 x 10(4) cells/cm2) rapidly proliferated and became polarized. Subsequently, supplemented media and air-liquid interface conditions resulted in development of highly differentiated epithelia composed of ciliated and nonciliated cells with gene expression characteristic of native airways. Genetically altered or injured mouse tracheal epithelial cells also reflected in vivo patterns of airway epithelial cell gene expression. Passage of cells resulted in continued proliferation but limited differentiation after the first passage, suggesting that transit-amplifying cell populations were present but with independent programs for proliferation and differentiation. This approach provides a high-fidelity in vitro model for evaluation of gene regulation and expression in mouse airway epithelial cells.
You
Yingjian
Y
Department of Medicine, Washington University School of Medicine, Saint Louis, Missouri 63110, USA.
Richer
Edward J
EJ
Huang
Tao
T
Brody
Steven L
SL
D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D002455:Cell Division; D002462:Cell Membrane / Q000648:ultrastructure; D016764:Cell Polarity / Q000502:physiology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D007700:Kinetics; D051379:Mice; D019108:Tight Junctions / Q000502:physiology; D014132:Trachea / Q000166:cytology* / Q000293:injuries / Q000502:physiology; D014945:Wound Healing / Q000502:physiology
D015415:Biomarkers