title: Detection of soluble HLA class I antigens by ELISA and determination of its reference value in population of Guangdong province.
abstract: OBJECTIVE
  To explore a method for quantitative detection of soluble human leukocyte antigen class I(HLA-I) antigens, with the assistance of which we attempt to define the reference value of the antigen in the population of Guangdong Province.
  
  
  METHODS
  Sandwich enzyme-linked immunosorbent assay (ELISA) was employed with W6/32 monoclonal antibody serving as the solid phase antibody and beta2 microglobulin antibody as the first antibody. HRP-labeled second antibody and the substrate were added for enzyme-catalyzed coloring. On the basis of the standard curve obtained by sHLA-I standard reagent in serial dilution, the concentration of sHLA-I antigens in the samples of 60 normal subjects from the population of Guangdong province was detected.
  
  
  RESULTS
  The sensitivity of this assay was 2.84 ng/ml with variation coefficients of 5.80% within the same batch and 9.00% between batches. The recovery rate ranged from 98.57% to 100.15%. The level of sHLA-I antigens in normal individuals was 699.54+/-360.10 ng/ml.
  
  
  CONCLUSIONS
  Sandwich ELISA is sensitive, specific and stable in the detection of sHLA-I.
authors.affiliation: Department of Transfusion, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Jiong-Cai
authors.initials: JC
authors.lastname: Lan
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Cong-Rong
authors.initials: CR
authors.lastname: Wang
authors.forename: Gao-Cai
authors.initials: GC
authors.lastname: Mao
mesh_terms: D000328:Adult; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility
chemical_list: D015395:Histocompatibility Antigens Class I