Frequent somatic mutations in PTEN and TP53 are mutually exclusive in the stroma of breast carcinomas.
We have recently shown that loss of heterozygosity of specific markers, including those at 10q23, 17p13-p15 and 16q24, can occur in the stromal and epithelial compartments of primary invasive breast carcinomas. Here, we demonstrate high frequencies of somatic mutations in TP53 (encoding tumor protein p53) and PTEN (encoding phosphate and tensin homolog) in breast neoplastic epithelium and stroma. Mutations in TP53 and PTEN are mutually exclusive in either compartment. In contrast, mutations in WFDC1 (16q24, encoding WAP four-disulfide core domain 1) occur with low frequency in the stroma.
Kurose
Keisuke
K
Comprehensive Cancer Center, and the Division of Human Genetics, Department of Internal Medicine, The Ohio State University, Columbus, Ohio 43210, USA.
Gilley
Kristie
K
Matsumoto
Satoshi
S
Watson
Peter H
PH
Zhou
Xiao-Ping
XP
Eng
Charis
C
D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D005091:Exons; D020868:Gene Silencing; D016158:Genes, p53; D006801:Humans; D019656:Loss of Heterozygosity; D008969:Molecular Sequence Data; D009154:Mutation; D009361:Neoplasm Invasiveness; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D011506:Proteins; D025521:Tumor Suppressor Proteins; C484310:WFDC1 protein, human; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human