title: Hydroxylamine reductase activity of the hybrid cluster protein from Escherichia coli.
abstract: The hybrid cluster protein (HCP; formerly termed the prismane protein) has been extensively studied due to its unique spectroscopic properties. Although the structural and spectroscopic characteristics are well defined, its enzymatic function, up to this point, has remained unidentified. While it was proposed that HCP acts in some step of nitrogen metabolism, a specific role for this enzyme remained unknown. Recent studies of HCP purified from Escherichia coli have identified a novel hydroxylamine reductase activity. These data reveal the ability of HCP to reduce hydroxylamine in vitro to form NH(3) and H(2)O. Further biochemical analyses were completed in order to determine the effects of various electron donors, different pH levels, and the presence of CN(-) on in vitro hydroxylamine reduction.
authors.affiliation: Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Marcus T
authors.initials: MT
authors.lastname: Wolfe
authors.forename: Jongyun
authors.initials: J
authors.lastname: Heo
authors.forename: John S
authors.initials: JS
authors.lastname: Garavelli
authors.forename: Paul W
authors.initials: PW
authors.lastname: Ludden
mesh_terms: D000042:Absorption; D001426:Bacterial Proteins / Q000378:metabolism*; D002248:Carbon Monoxide; D003486:Cyanides; D004926:Escherichia coli / Q000201:enzymology*; D019811:Hydroxylamine / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D010100:Oxygen; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D003486:Cyanides; D007506:Iron-Sulfur Proteins; C075199:prismane protein, Desulfovibrio vulgaris; D019811:Hydroxylamine; D002248:Carbon Monoxide; D010088:Oxidoreductases; C021402:hydroxylamine reductase; D010100:Oxygen