Characterization of a new HLA-A allele, A*0256, identified in a Caucasian individual.
HLA-A typing by the PCR-SSP method in a male 21-year-old Caucasian individual revealed a very rare allele combination corresponding to a unique reaction pattern. Therefore, the result was examined using sequence-based typing. Sequencing of exons 2 and 3 of the HLA-A locus after allelic separation with specific primers revealed the sequence of a new allele, similar to A*0245. Sequencing of exons 1 and 4 resulted in no additional inconclusive positions. The sequence pattern of the new allele HLA-A*0256 might have been generated as a result of a double crossing over recombination of an A*0201 and either an A*03 or an A*11.
Czachurski
D
D
Department of Transplantation Immunology, Institute of Immunology, University of Heidelberg, Germany.
Rausch
M
M
Scherer
S
S
Opelz
G
G
Mytilineos
J
J
D000328:Adult; D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D015234:HLA-A Antigens / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D044465:White People / Q000235:genetics*
D017931:DNA Primers; D015234:HLA-A Antigens