Characterisation of immunodominant protein encoded by the F1L gene of orf virus strains isolated in Italy.
We analysed the molecular properties of the immunodominant protein of different orf virus strains isolated in Italy. The F1L encoding genes and the deduced amino acid sequences of all strains were determined and compared, and they showed several mutations. Structural analysis was carried out in order to assess the influence of amino acid variations on protein structure demonstrating a conservation of the secondary structure. Western blot analysis and immunogold electron microscopy showed that all orf virus strains were antigenically identical. The results of our study confirmed the immunogenicity of the F1L protein; furthermore, our data suggest a possible involvement of the protein in the virus cycle.
Scagliarini
A
A
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale, Facoltá di Medicina Veterinaria, Università degli Studi di Bologna, Bologna, Italy. ascagliarini@vet.unibo.it
Ciulli
S
S
Battilani
M
M
Jacoboni
I
I
Montesi
F
F
Casadio
R
R
Prosperi
S
S
D000595:Amino Acid Sequence; D000818:Animals; D004474:Ecthyma, Contagious / Q000821:virology*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D011212:Poxviridae / Q000737:chemistry* / Q000235:genetics; D017433:Protein Structure, Secondary; D012756:Sheep; D014764:Viral Proteins / Q000737:chemistry* / Q000276:immunology
D014764:Viral Proteins