Sonodynamic effect of erythrosin B on sarcoma 180 cells in vitro.
The ultrasonically induced cytotoxic effect of erythrosin B (EB) on isolated sarcoma 180 cells was investigated. The tumor cells were suspended in an air-saturated phosphate buffered saline and exposed to ultrasound at 1.93 MHz in a standing-wave mode for up to 60 s in the presence and absence of EB. The rate of cell damage induction by ultrasound was enhanced by 4-5 times with 160-microM EB, while no cell damage was observed with EB alone. This enhancement was significantly inhibited by histidine. Sonochemical generation of active oxygen species in the presence of EB, measured by ESR spectroscopy, was also inhibited by histidine. These results indicate the involvement of a sonochemical mechanism.
Yumita
Nagahiko
N
School of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
Kawabata
Ken-ichi
K
Sasaki
Kazuaki
K
Umemura
Shin-ichiro
S
D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D004578:Electron Spin Resonance Spectroscopy; D004923:Erythrosine / Q000378:metabolism* / Q000494:pharmacology* / Q000627:therapeutic use; D006639:Histidine / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010100:Oxygen / Q000378:metabolism; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000473:pathology; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014464:Ultrasonic Therapy
D000970:Antineoplastic Agents; D006639:Histidine; D004923:Erythrosine; D010100:Oxygen