Volumetric measurement of the hippocampus, the anterior cingulate cortex, and the retrosplenial granular cortex of the rat using structural MRI.
MRI imaging of the rodent brain is a rapidly growing field in the neurosciences. Relatively limited information is available for regional volume determination. The present paper describes a reliable method for the assessment of the hippocampus, the anterior cingulate cortex, the retrosplenial granular cortex and the ventricles in rats. MRI scans were acquired using a 7 T magnet. The anatomical sampling method was found to be highly reliable with an intra-rater reliability of greater than 0.93. The current protocol should facilitate future in vivo neuroimaging research using animal models of neurodegenerative diseases.
Wolf
O T
OT
Center for Brain Health, Neuroimaging Laboratory, NYU School of Medicine, New York, NY 10016, USA. oliver.wolf@uni-duesseldorf.de
Dyakin
V
V
Vadasz
C
C
de Leon
M J
MJ
McEwen
B S
BS
Bulloch
K
K
D000818:Animals; D001931:Brain Mapping / Q000295:instrumentation / Q000379:methods*; D003337:Corpus Callosum / Q000033:anatomy & histology; D006179:Gyrus Cinguli / Q000033:anatomy & histology*; D006624:Hippocampus / Q000033:anatomy & histology*; D020547:Lateral Ventricles / Q000033:anatomy & histology*; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods*; D008297:Male; D015588:Observer Variation; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000033:anatomy & histology*; D015203:Reproducibility of Results