title: From one well to 9000: using high-density streptavidin-coated membranes for kinase detection.
abstract: The study of kinases and their role in cellular regulation continues to expand as the human genome is sequenced and new kinases are identified as expression products of newly discovered genes. Reagents and assay systems that allow for sensitive, accurate, and high-throughput analysis of both purified kinases as well as crude extracts will enhance the characterization of these important cellular components and will speed the identification of appropriate therapeutic targets and the development of new and more effective treatments.
authors.affiliation: Promega Corporation, Madison, WI 53711, USA. mcurtin@promega.com
authors.forename: Michael
authors.initials: M
authors.lastname: Curtin
authors.forename: John
authors.initials: J
authors.lastname: Watson
authors.forename: Said
authors.initials: S
authors.lastname: Goueli
mesh_terms: D001345:Autoradiography; D001710:Biotin / Q000378:metabolism*; D019921:Biotinylation; D003720:Densitometry; D015195:Drug Design; D006801:Humans; D008567:Membranes, Artificial; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D009363:Neoplasm Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D010761:Phosphorus Radioisotopes / Q000032:analysis*; D010766:Phosphorylation; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011485:Protein Binding; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000032:analysis* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012588:Scintillation Counting; D019809:Streptavidin / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D008567:Membranes, Artificial; D009363:Neoplasm Proteins; D010455:Peptides; D010761:Phosphorus Radioisotopes; D047428:Protein Kinase Inhibitors; D001710:Biotin; D019809:Streptavidin; D011494:Protein Kinases