Human antibody response to Toscana virus glycoproteins expressed by recombinant baculovirus.
The arthropod-borne Toscana virus has been associated with acute neurological disease in humans. In this study, the viral envelope glycoproteins were expressed in soluble form in a baculovirus system. The recombinant sGN and sGC proteins were used as viral antigens in a Western blot assay to analyze the specific immune response in sera from patients with recognized virus-associated aseptic meningitis. The anti-glycoprotein and the anti-nucleoprotein N IgG responses were compared by an immunoassay based on the recombinant proteins. In this system, all the sera showed a high reactivity to the N protein, but they differed in the response to the glycoproteins.
Di Bonito
Paola
P
Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy.
Bosco
Simona
S
Mochi
Stefania
S
Accardi
Luisa
L
Ciufolini
Maria G
MG
Nicoletti
Loredana
L
Giorgi
Colomba
C
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D016367:Baculoviridae / Q000235:genetics; D015153:Blotting, Western; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000096:biosynthesis / Q000276:immunology; D010217:Phlebotomus Fever / Q000175:diagnosis / Q000276:immunology*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D029301:Sandfly fever Naples virus / Q000235:genetics / Q000276:immunology*; D018411:Spodoptera; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000276:immunology*
D000914:Antibodies, Viral; D007106:Immune Sera; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins