Detection of activity of telomerase in tumor cells using fiber optical biosensors.
Human telomerase plays an important role in the cancerogenesis as it is up-regulated in 80-90% of malignant tumors. Thus, it is considered as a potential cancer marker and relevant target in oncology. Its task is the extension of guanine-rich strands of the telomere using an intrinsic RNA as the template. In this paper we developed a new biosensoric assay based on total internal reflection fluorescence measuring the activity of the telomerase on sensor surface. Two alternatives to determine the telomeric activity are demonstrated without the use of amplifying steps as e.g. PCR. The enzymatic inclusion of FITC-labeled dUTPs should reveal the synthesis process in real-time indicating the elongation of a phosphothioate telomeric substrate (PS/TS)-modified primer. Additionally the elongated strand was detected by hybridization with a FITC-labeled complementary linear DNA probe. As the telomeric guanine-rich single-stranded DNA adopts intramolecular quadruplex structures, it was necessary for the hybridization to linearize the telomeric DNA by increasing the reaction temperature to 48 degrees C. The comparison of the telomerase activity using labeled and unlabeled nucleotides indicated the inhibition effect of the FITC-labeled nucleotides slowing down the synthesis rate of the enzyme. It is shown with the modified biosensor that the PS/TS primer binds the telomerase from the HL-60 cell lysates, effectively elongating the immobilized primer. Furthermore no more purification steps were required as all measurements were performed with crude cell extract.
Schmidt
Peter M
PM
Fraunhofer-Institut für Biomedizinische Technik, Abt. Molekulare Bioanalytik und Bioelektronik, A. Scheunert-Allee 114-116, 14558 Potsdam-Rehbrücke, Germany.
Lehmann
Christine
C
Matthes
Eckart
E
Bier
Frank F
FF
D014408:Biomarkers, Tumor / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002457:Cell Extracts / Q000032:analysis; D017931:DNA Primers; D004789:Enzyme Activation; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D005453:Fluorescence; D018922:HL-60 Cells / Q000201:enzymology; D006801:Humans; D012680:Sensitivity and Specificity; D019098:Telomerase / Q000032:analysis* / Q000378:metabolism
D014408:Biomarkers, Tumor; D002457:Cell Extracts; D017931:DNA Primers; D019098:Telomerase