[Histamine receptors expression on lymphocytes and neutrophils in glucocorticosteroid sensitive and resistant asthma patients].
Histamine is a physiological mediator which exerts both, effector and regulatory influence through its receptors on various cells. In the study we evaluated the expression of histamine receptors (HR) on inflammatory cells (lymphocytes and neutrophils) in glucocorticosteroid sensitive (n. = 17) and resistant (n. = 19) asthmatic patients and healthy subjects (n = 14). Moreover, we examined in vitro the influence of cortisol on HR expression in both studied groups with asthma and in a control group. We concluded that HR expression on lymphocytes was similar in all studied subjects. However, HR expression on neutrophils in both groups of asthmatic patients was significantly decreased compared to the control group. After the in vitro incubation with cortisol, we observed a significant decrease in HR expression on neutrophils (10(-8)-10(-5) M) and lymphocytes (10(-8)-10(-4) M) only in healthy subjects. Taking into consideration that histamine proinflammatory action is mediated by H1 receptor, and that in patients treated with long-time systemic GCS therapy we did not observe any decrease in HR expression in vitro, we suggest additional therapy with the second-generation antihistamines. These drugs not only block H1 receptors, but also have an anti-inflammatory effect.
Zak-Nejmark
Teresa
T
Katedra i Klinika Chorób Wewnetrznych i Alergologii AM we Wrocławiu.
Wytrychowski
Krzysztof
K
Patkowski
Janusz
J
Nadobna
Grazyna
G
Małolepszy
Józef
J
D000328:Adult; D000368:Aged; D000893:Anti-Inflammatory Agents / Q000097:blood / Q000494:pharmacology* / Q000627:therapeutic use; D001249:Asthma / Q000097:blood / Q000188:drug therapy*; D016022:Case-Control Studies; D004351:Drug Resistance; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood / Q000494:pharmacology* / Q000627:therapeutic use; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011968:Receptors, Histamine / Q000187:drug effects* / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D011968:Receptors, Histamine; D006854:Hydrocortisone