[Identification of genes induced to chondrocytes by nitric oxide].
Nitric oxide (NO) acts as a short-lived paracrine factor and selectively activates transcription of certain genes. The spectrum of inducible genes was studied in primary chondrocytes. A cDNA library was obtained by subtraction hybridization with RNAs isolated from rabbit chondrocytes before and after treatment with nitrosoglutathione, an NO-generating agent. Some of the cloned cDNAs were homologous to known mammalian genes and human EST. NO-dependent transcriptional activation was demonstrated for the stromelysin 1 and cyclooxygenase 2 genes and, for the first time, for mcl1 coding for an apoptosis suppressor.
Nikolaev
A I
AI
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia.
Dubovaia
V I
VI
Litvinov
D Iu
DIu
Poltaraus
A B
AB
Ivanov
D S
DS
Amchenkova
A M
AM
Narovlianskiĭ
A N
AN
Panasiuk
A F
AF
Prasolov
V S
VS
Turpaev
K T
KT
D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology*; D051546:Cyclooxygenase 2; D018076:DNA, Complementary; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D015723:Gene Library; D017403:In Situ Hybridization / Q000379:methods; D007527:Isoenzymes / Q000235:genetics; D019278:Matrix Metalloproteinase 3 / Q000235:genetics; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins / Q000187:drug effects / Q000235:genetics; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology; D020030:Nitric Oxide Donors / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D026422:S-Nitrosoglutathione / Q000494:pharmacology
D018076:DNA, Complementary; D007527:Isoenzymes; C579093:Mcl1 protein, rat; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins; D020030:Nitric Oxide Donors; D019253:Proto-Oncogene Proteins c-bcl-2; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D019278:Matrix Metalloproteinase 3