title: Nitric oxide breath testing by tunable-diode laser absorption spectroscopy: application in monitoring respiratory inflammation.
abstract: We used a high-resolution mid-IR tunable-laser absorption spectroscopy (TLAS) system with a single IV-VI laser operating near 5.2 microm to measure the level of exhaled nitric oxide (eNO) in human breath. A method of internal calibration using simultaneous eNO and exhaled CO2 measurements eliminated the need for system calibration with gas standards. The results observed from internally calibrating the instrument for eNO measurements were compared with measurements of eNO calibrated to gas standards and were found to be similar. Various parameters of the TLAS system for eNO breath testing were examined and include gas cell pressure, exhalation time, and ambient NO concentrations. A reduction in eNO from elevated concentrations (approximately 44 parts in 10(9)) to near-normal levels (<20 parts in 10(9)) from an asthmatic patient was observed after the patient had received treatment with an inhaled glucocorticoid anti-inflammatory medication. Such measurements can help in evaluating airway inflammation and in monitoring the effectiveness of anti-inflammatory therapies.
authors.affiliation: Ekips Technologies, Inc, Norman, Oklahoma 73069, USA.
authors.forename: Chad
authors.initials: C
authors.lastname: Roller
authors.forename: Khosrow
authors.initials: K
authors.lastname: Namjou
authors.forename: James D
authors.initials: JD
authors.lastname: Jeffers
authors.forename: Mark
authors.initials: M
authors.lastname: Camp
authors.forename: Adam
authors.initials: A
authors.lastname: Mock
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: McCann
authors.forename: Joe
authors.initials: J
authors.lastname: Grego
mesh_terms: D000042:Absorption; D001249:Asthma / Q000503:physiopathology*; D001944:Breath Tests / Q000295:instrumentation* / Q000379:methods*; D002245:Carbon Dioxide; D006801:Humans; D007834:Lasers; D009569:Nitric Oxide; D014867:Water
chemical_list: D014867:Water; D002245:Carbon Dioxide; D009569:Nitric Oxide