Extracellular metabolisation of NADH by blood cells correlates with intracellular ATP levels.
A new assay allowing quantitation of extracellular NADH metabolisation by intact blood cells was compared with the intracellular ATP/ADP ratio of these cells. The sensitivity, reproducibility and NADH specificity of this assay were determined. The diagnostic potential of this test was examined in a study with highly conditioned athletes. NADH consumption was measured before and immediately after maximum aerobic performance as well as 1 day later and was compared with the ATP/ADP level in these blood cells. A significant decline of cellular energy after aerobic performance was detected with both approaches to a similar extent (P<0.01). However, the extracellular NADH metabolisation assay (ENMA) is more convenient to perform than the determination of intracellular ATP/ADP. Due to its easy and versatile handling, a huge array of possible applications like monitoring the training efficiency of athletes, the fitness of senior citizens or the recovery from disease may be envisioned.
Nadlinger
Karl
K
Department of Research and Development, Birkmayer Laboratories, Schwarzspanierstrasse 15, A-1090 Vienna, Austria. K.Nadlinger@birkmeyer.com
Westerthaler
Wilhelm
W
Storga-Tomic
Danijela
D
Birkmayer
Jörg G D
JG
D000244:Adenosine Diphosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D001773:Blood Cells / Q000378:metabolism*; D015444:Exercise / Q000502:physiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D009243:NAD / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D015203:Reproducibility of Results; D013048:Specimen Handling
D009243:NAD; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate