Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice.
The kinesin superfamily proteins (KIFs) play essential roles in receptor transportation along the microtubules. KIF17 transports the N-methyl-d-aspartate receptor NR2B subunit in vitro, but its role in vivo is unknown. To clarify this role, we generated transgenic mice overexpressing KIF17 tagged with GFP. The KIF17 transgenic mice exhibited enhanced learning and memory in a series of behavioral tasks, up-regulated NR2B expression with the potential involvement of a transcriptional factor, the cAMP-dependent response element-binding protein, and increased phosphorylation of the cAMP-dependent response element-binding protein. Our results suggest that the motor protein KIF17 contributes to neuronal events required for learning and memory by trafficking fundamental N-methyl-d-aspartate-type glutamate receptors.
Wong
Richard Wing-Chuen
RW
Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Setou
Mitsutoshi
M
Teng
Junlin
J
Takei
Yosuke
Y
Hirokawa
Nobutaka
N
D000818:Animals; D001483:Base Sequence; D001522:Behavior, Animal; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression; D016547:Kinesins / Q000096:biosynthesis* / Q000235:genetics; D018782:Maze Learning; D008568:Memory / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D020409:Molecular Motor Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D016548:Prosencephalon / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D013647:Task Performance and Analysis
D017362:Cyclic AMP Response Element-Binding Protein; D018076:DNA, Complementary; C409642:KIF17 protein, mouse; D020409:Molecular Motor Proteins; C120997:NR2A NMDA receptor; C121001:NR2B NMDA receptor; D016194:Receptors, N-Methyl-D-Aspartate; D016547:Kinesins