Intrinsic degradation of volatile fatty acids in laboratory-compacted clayey soil.
Volatile fatty acids (VFAs) represent the major organic constituent of landfill leachate and provide the greatest potential for leachate induced organic contamination of groundwater (e.g. as represented by an increase in the concentration of dissolved organic carbon and chemical oxygen demand). Long-term diffusion tests were performed for laboratory-compacted clayey soil plugs exposed to continuous supply of synthetic leachate containing VFAs. Significant microbial activity developed upon exposure of the soil's indigenous microorganisms to these degradable contaminants. The growth of heterotrophic aerobic bacteria (HAB, which include facultative anaerobes), sulfate reducing bacteria (SRB) and methanogenic bacteria carrying out fermentation and mineralization of the VFAs became evident after 30-50 days of testing. The maximum microbial counts of (2-8) x 10(8) and (0.1-1) x 10(8) cfu/g for HAB and SRB were localized in the soil layer at the interface with the source of organic and inorganic nutrients. Regardless of this rapid growth in microbial population, the VFA consumption was small and measurable only after a lag of 140-180 days. It is considered that this lag of otherwise readily degradable organic compounds (such as VFAs) persisted due to a combination of the effects of a high initial concentration of these acids (2.4 g/l as dissolved organic carbon, DOC) applied to carbon starved soil microorganisms and the small pore size of the compacted clay. Once the significant amounts of gas were generated from fermentation, conditions developed for improved mass transport and exchange of the nutrients and bacteria and the outcome of the intrinsic degradation was more apparent. The breakdown of VFAs that followed after the lag was localized near the top of the soil and was characterized by a short half-life of 0.75-5 days for DOC (total VFAs as dissolved organic carbon).
Hrapovic
L
L
Department of Civil and Environmental Engineering, The University of Western Ontario, London, Canada.
Rowe
R K
RK
D000538:Aluminum Silicates / Q000737:chemistry; D001420:Bacteria, Aerobic / Q000254:growth & development / Q000378:metabolism; D000077215:Clay; D019605:Euryarchaeota / Q000254:growth & development / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D012037:Refuse Disposal / Q000379:methods*; D012988:Soil Microbiology; D012989:Soil Pollutants; D016999:Sulfur-Reducing Bacteria / Q000254:growth & development / Q000378:metabolism
D000538:Aluminum Silicates; D005232:Fatty Acids, Volatile; D012989:Soil Pollutants; D000077215:Clay