TPA stimulation culture for improved detection of t(11;14)(q13;q32) in mantle cell lymphoma.
Cytogenetic analysis of mantle cell lymphoma (MCL), characterized by the presence of t(11;14)(q13;q32) translocation, is often difficult because of the low proliferating rate of MCL cells and the presence of normal cells in bone marrow which may interfere with growth of MCL cells. We describe herein a TPA (12-O-tetradecanoylphorbol 13-acetate) stimulated culture to improve detection of t(11;14)(q13;q32) in 20 MCL patients regardless of the samples used.
Barouk-Simonet
Emmanuelle
E
Laboratoire de Génétique Médicale, Barre Nord Hôpital Jeanne de Flandre, CHRU de Lille, Lille, France.
Andrieux
Joris
J
Copin
Marie-Christine
MC
Grardel-Duflos
Nathalie
N
Huyghe
Pauline
P
Patte
Jean-Hugues
JH
Preudhomme
Claude
C
Quesnel
Bruno
B
Laï
Jean-Luc
JL
D000328:Adult; D000368:Aged; D018929:Cell Culture Techniques / Q000379:methods; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D005260:Female; D006801:Humans; D020522:Lymphoma, Mantle-Cell / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D013755:Tetradecanoylphorbol Acetate