A direct method for regiospecific analysis of TAG using alpha-MAG.
An analytical procedure was developed for regiodistribution analysis of TAG using alpha-MAG prepared by an ethyl magnesium bromide deacylation. In the present communication, the deacylation procedure is shown to lead to representative alpha-MAG, allowing the composition of the native TAG in the alpha-position to be determined directly. The composition in the beta-position can then be estimated from the composition of the alpha-MAG and TAG according to the formula 3 x TAG - 2 x alpha-MAG. The estimates are superior to those obtained using the alpha,beta-DAG and Brockerhoff calculations as they come closer to the theoretical value and have smaller SD. The present procedure, first demonstrated on a synthetic TAG, was then successfully applied to the analysis of borage oil, milkfat, and tuna oil.
Turon
F
F
Sea Oil, Beaumont-Hague, France. fturon@danone.com
Bachain
P
P
Caro
Y
Y
Pina
M
M
Graille
J
J
D000215:Acylation; D002623:Chemistry Techniques, Analytical / Q000379:methods; D004041:Dietary Fats / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D005395:Fish Oils / Q000032:analysis; D005989:Glycerides / Q000737:chemistry*; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism; D009942:Organometallic Compounds / Q000737:chemistry; D010938:Plant Oils / Q000032:analysis; D015203:Reproducibility of Results; D013237:Stereoisomerism; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry; D017965:gamma-Linolenic Acid
D004041:Dietary Fats; D005227:Fatty Acids; D005395:Fish Oils; D005989:Glycerides; D009942:Organometallic Compounds; D010938:Plant Oils; D014280:Triglycerides; D017965:gamma-Linolenic Acid; C067783:ethyl magnesium bromide; D008049:Lipase; C079363:borage oil