A new method of implanting orthotopic rat bladder tumor for experimental therapies.
We developed a model of orthotopic transplantation of bladder tumor cells in female Fischer rats using a new reproducible technique. After first performing the mechanical abrasion of a portion of the bladder urothelium with an Abrader inserted transurethrally via a catheter, we administered a suspension of 5-40 x 10(6) viable AY-27 tumor cells in sterile phosphate-buffered saline to the bladder cavity. This rapidly led to a tumor growth incidence of approximately 100%. The induced bladder tumors grew expansively into the bladder cavity from the surface (mucosa) and gradually invaded the submucosa, muscles, serosa and surrounding tissue (high-stage invasive transitional cell carcinoma). Size and staging were related to the quantity of tumor cells instilled into the bladder cavity. This model matches the characteristics of human bladder tumor more closely than other bladder cancer models induced with tumor cells. Moreover, it presents many advantages: the method is reproducible, tumors grow rapidly, they are directly attached to the bladder surface and they are always located on the bladder wall, in line with the urethra. This proves especially helpful for evaluating chemotherapeutic agents by different means such as in vivo fluorescence spectroscopy, a noninvasive method used in photodynamic therapy, or other methods designed to detect and treat transitional cell carcinoma.
Bisson
Jean-François
JF
Laboratoire d'Hématologie, Physiologie et Biologie Cellulaire, Faculté de Pharmacie, Université Henri Poincaré-Nancy I, 5-7 rue Albert Lebrun, 54001 Nancy Cedex, France.
Parache
Robert M
RM
Droulle
Pierre
P
Notter
Dominique
D
Vigneron
Claude
C
Guillemin
François
F
D000818:Animals; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D009368:Neoplasm Transplantation; D051381:Rats; D011916:Rats, Inbred F344; D035703:Therapies, Investigational / Q000379:methods; D014407:Tumor Cells, Cultured; D014463:Ultrasonography; D001743:Urinary Bladder / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology*