title: The obligate root parasite Orobanche cumana exhibits several rbcL sequences.
abstract: Orobanche species characterization using plastid sequences as molecular markers revealed that O. cumana contains at least two distinct rbcL sequences: one similar in size to the truncated rbcL pseudogene from O. cernua, a closely related species, and another with a size comparable to that of rbcL plastid genes from autotrophic plants. In this work, the nucleotide sequences of these two copies are reported and analysed. The organization of the O. cumana plastid genome was investigated using a long-distance PCR strategy in order to determine their localization. Because of the non-plastid localization of the rbcL larger copy, Southern blot and PCR chromosome-walking experiments were carried out to better characterize this transferred sequence and to identify its localization. Then the mode of multiple transfer of genetic information from plastid to nucleus and the concomitant plastid sequence disorganization and migration during parasitic plant evolution are discussed.
authors.affiliation: Groupe de Physiologie et Pathologie Végétales, Faculté des Sciences et des Techniques, BP 92208, 2 rue de la Houssinière, 44322 Cedex 3, Nantes, France. delavault@svt.univ-nantes.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Delavault
authors.forename: Patrick
authors.initials: P
authors.lastname: Thalouarn
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D016386:Chromosome Walking; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D018745:Genome, Plant; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D031672:Orobanche / Q000201:enzymology / Q000235:genetics*; D018087:Plastids / Q000235:genetics / Q000378:metabolism; D011544:Pseudogenes / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018744:DNA, Plant; D007527:Isoenzymes; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase