title: Local and systemic autonomic nervous effects on cell migration to the spleen.
abstract: This work is based on the hypothesis that sympathetic nerves regulate the uptake of circulating cells by the spleen by affecting splenic blood flow and that the quantity of cells sequestered depends on whether changes in noradrenergic transmission occur at local or systemic levels. Fluorescently labeled lymphoid cells were injected into rats, and organ blood flow was measured by the microsphere method. Increased retention of cells in the spleen paralleled by increased blood flow was detected after local denervation of this organ or administration of bacterial endotoxin. A comparable enhanced splenic blood flow was observed after general sympathectomy. However, the redistribution of blood perfusion during general vasodilatation resulted in deviation of leukocyte flow from the spleen, thus resulting in reduced uptake of cells by this organ. These results indicate that, although the uptake of cells by the spleen depends on arterial blood supply, enhanced perfusion does not always result in increased cell sequestration because general vasodilatation reduces cell uptake by this organ and even overrides stimulatory effects of endotoxin.
authors.affiliation: Department of Immunophysiology, Institute of Physiology, Philipps-University, 35039 Marburg, Germany.
authors.forename: Heiner
authors.initials: H
authors.lastname: Rogausch
authors.forename: Detlev
authors.initials: D
authors.lastname: Zwingmann
authors.forename: Mirjam
authors.initials: M
authors.lastname: Trudewind
authors.forename: Adriana
authors.initials: A
authors.lastname: del Rey
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Voigt
authors.forename: Hugo
authors.initials: H
authors.lastname: Besedovsky
mesh_terms: D000818:Animals; D001341:Autonomic Nervous System / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D003714:Denervation; D008070:Lipopolysaccharides / Q000494:pharmacology; D008221:Lymphoid Tissue / Q000166:cytology / Q000502:physiology; D008297:Male; D051381:Rats; D011921:Rats, Inbred WKY; D012039:Regional Blood Flow / Q000187:drug effects / Q000502:physiology; D013154:Spleen / Q000098:blood supply* / Q000294:innervation / Q000502:physiology*; D013562:Sympathectomy; D014664:Vasodilation / Q000502:physiology
chemical_list: D008070:Lipopolysaccharides