title: The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.
abstract: Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semi-conservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids.
authors.affiliation: Plant Breeding and Acclimatization Institute, Radzików, 05-870 Błonie, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Przetakiewicz
authors.forename: Anna
authors.initials: A
authors.lastname: Nadolska-Orczyk
authors.forename: Wacław
authors.initials: W
authors.lastname: Orczyk
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004171:Diploidy; D005819:Genetic Markers; D006824:Hybridization, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D011198:Solanum tuberosum / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers