title: [Conserved interactions of the active carboxyls in pepsin-like enzymes and retroviral proteases].
abstract: In addition to previous studies, 30 crystal structures of retroviral proteases corresponding to the highest resolution were inspected to analyze the interactions of the active carboxyl with surroundings groups. The outer oxygen of the active carboxyl in retroviral enzymes form contacts only with the water molecule participating in catalysis. This is an important difference between retroviral proteases and pepsin-like enzymes, which form a net of hydrogen bonds of these outer oxygen with residues neighboring the catalytic site in 3D structures. At the same time, it was found that in all aspartic proteases the inner oxygen of the active carboxyl are also involved in the chain of interactions through peptide groups Thr-Gly adjacent to the active residues. Polarization of these peptide groups influences the donor-acceptor properties of the active carboxyl. The found chain of interactions is more extensive in retroviral than in pepsin-like proteases; however, its main part is conserved for the whole class of these enzymes. Some implications of the role of these interactions are discussed.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia. andreeva@genome.eimb.relarn.ru
authors.forename: N S
authors.initials: NS
authors.lastname: Andreeva
authors.forename: M E
authors.initials: ME
authors.lastname: Popov
mesh_terms: D001224:Aspartic Acid / Q000737:chemistry*; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000378:metabolism; D030562:Databases, Protein; D010450:Endopeptidases / Q000737:chemistry* / Q000378:metabolism*; D005998:Glycine / Q000737:chemistry; D010434:Pepsin A / Q000737:chemistry* / Q000378:metabolism; D012190:Retroviridae / Q000201:enzymology*; D040681:Structural Homology, Protein; D013912:Threonine / Q000737:chemistry
chemical_list: D013912:Threonine; D001224:Aspartic Acid; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; D010434:Pepsin A; D005998:Glycine