Impaired passive avoidance acquisition in Sprague-Dawley and Lewis rats after restraint and cold stress.
The study examined the effects of restraint combined with cold water stress (IMO+C) on learning and memory of Sprague-Dawley (S-D) and Lewis (LE) rats in the passive avoidance task. The procedure started with 6 days of adaptation to the apparatus during which the recorded latencies to enter the dark compartment were used to assess the process of habituation. On the training day rats were exposed to IMO+C for 60 min and the stressor exposure terminated 1 h before the acquisition trial. Retention trials started 24 h later. To evaluate the possible long-term consequences of the acute and repeated stress presentation on the performance of the two strains with diverse activity of hypothalamic-pituitary-adrenal axis, this procedure was performed three times including stress application (Parts 1-3). Finally, an identical procedure was performed without stress (Part 4). An immediate behavioural effect of the stressor exposure was observed in an increase of latencies to enter the dark compartment before the shock delivery in rats of both strains; this enhancement approached significance after the second and third exposure to the stressor (Parts 2 and 3). Control animals of both strains acquired passive avoidance response after training in Parts 2-4. IMO+C produced significant impairment of this response irrespective of the strain. The three-time repeated exposure did not influence the ability to learn the task in the final procedure without stress. Differences in behaviour of S-D and LE rats were observed already during the first adaptation period. LE rats exhibited longer latencies upon the first exposure to the novel environment compared to S-D rats. Also only LE rats displayed habituation. In Part 4 marked strain differences in the latencies both before and after training were recorded. The results show that the repeated exposure to the IMO+C stressor proved to be a strong amnestic stimulus but without persisting consequences for the ability of rats to acquire the learning task.
Klenerová
Vera
V
Charles University in Prague, First Medical Faculty, Department of Pharmacology, Albertov 4, Czech Republic. vera.klenerova@1fl.cuni.cz
Kaminský
Ondrej
O
Sída
Pavel
P
Krejcí
Ivan
I
Hlinák
Zdenek
Z
Hynie
Sixtus
S
D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D003080:Cold Temperature; D004597:Electroshock; D007101:Immersion; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D013045:Species Specificity; D013315:Stress, Psychological / Q000523:psychology*