title: Apoptosis induced by culturing MH134 cells in the presence of porcine skin gelatin in vitro.
abstract: Porcine skin (PS) gelatin showed antitumor effect in vitro on MH134 murine hepatoma cells. We analyzed the effect of PS gelatin on MH134 cells compared to the effect of Bovine bone (BB) gelatin, 5-Fluorouracil (5-FU) and 7-ethyl-10-hydroxycamptothecin (SN-38). We previously suggested that PS gelatin induces apoptosis in MH134 cells using flow cytometric analysis. We performed agarose gel electrophoresis and electron microscopic studies to ascertain apoptosis. Agarose gel electrophoresis showed the typical DNA ladder pattern and electron microscopic findings revealed characteristic features in the case of apoptosis on PS gelatin. SN-38 also showed DNA ladder pattern and ultrastructural changes in apoptosis. 5-FU didn't show DNA ladder pattern but electron microscope revealed changes in necrosis. On the other hand, BB gelatin didn't induce apoptosis or necrosis.
authors.affiliation: Department of Surgery, Aichi Medical University, Nagakute-cyo, Japan. tetsu-i@t92.so-net.ne.jp
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ito
authors.forename: Takashi
authors.initials: T
authors.lastname: Kojima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nagata
authors.forename: Norio
authors.initials: N
authors.lastname: Ozeki
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nonami
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000032:analysis; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation; D005434:Flow Cytometry; D005780:Gelatin / Q000494:pharmacology*; D051379:Mice; D008854:Microscopy, Electron; D013552:Swine; D014407:Tumor Cells, Cultured
chemical_list: D017304:Annexin A5; D000970:Antineoplastic Agents; D005780:Gelatin