title: Assessment of bioaccumulation, neuropathology, and neurobehavior following subchronic (90 days) inhalation in Sprague-Dawley rats exposed to manganese phosphate.
abstract: Methylcyclopentadienyl manganese tricarbonyl (MMT) is an organic manganese (Mn) compound added to unleaded gasoline. It has been suggested that the combustion products of MMT containing Mn, such as manganese phosphate, could cause neurological symptoms similar to Parkinson's disease in humans. The aim of this work was to investigate the exposure-response relationship of bioaccumulation, neuropathology, and neurobehavior following a subchronic inhalation exposure to manganese phosphate in Sprague-Dawley male rats. Rats were exposed 6 h/day, 5 days/week for 13 consecutive weeks at 30, 300, or 3000 microg/m(3) Mn phosphate and compared to controls. Some rats were implanted with chronic EMG electrodes in the gastrocnemius muscle of the hind limb to assess tremor at the end of Mn exposure. Spontaneous motor activity was measured for 36 h using a computerized autotrack system. Rats were then sacrificed by exsanguination and Mn level in different brain tissues and other organs was determined by instrumental neutron activation analysis. Neuronal cell counts were obtained by assessing the sum of five grid areas for the caudate/putamen and the sum of two adjacent areas for the globus pallidus. Increased manganese concentrations were observed in all tissues of the brain and was dose-dependent in olfactory bulb and caudate/putamen. In fact, beginning with the highest level of exposure (3000 microg/m(3)) and ending with the control group, Mn concentrations in the olfactory bulb were 2.47 vs 1.28 vs 0.77 vs 0.64 ppm (P < 0.05) while for the caudate/putamen, Mn concentrations were 1.06 vs 0.73 vs 0.62 vs 0.47 ppm (P < 0.05). The Mn concentrations in lung were also dose-dependent (10.30 vs 1.40 vs 0.42 vs 0.17 ppm; P < 0.05). No statistical difference was observed for loss of neurons in caudate/putamen and globus pallidus. Locomotor activity assessment and tremor assessment did not reveal in neurobehavioral changes between the groups. Our results reinforce the hypothesis that the olfactory bulb and caudate/putamen are the main brain tissues for Mn accumulation after subchronic inhalation exposure.
authors.affiliation: TOXHUM (Human Toxicology Research Group), Department of Environmental and Occupational Health, Faculty of Medicine, Université de Montréal, C.P. 6128, Succursale Centre-Ville, Montréal, Québec, Canada H3C 3J7.
authors.forename: Louise
authors.initials: L
authors.lastname: Normandin
authors.forename: Gaétan
authors.initials: G
authors.lastname: Carrier
authors.forename: Phillip F
authors.initials: PF
authors.lastname: Gardiner
authors.forename: Greg
authors.initials: G
authors.lastname: Kennedy
authors.forename: Alan S
authors.initials: AS
authors.lastname: Hazell
authors.forename: Donna
authors.initials: D
authors.lastname: Mergler
authors.forename: Roger F
authors.initials: RF
authors.lastname: Butterworth
authors.forename: Suzanne
authors.initials: S
authors.lastname: Philippe
authors.forename: Joseph
authors.initials: J
authors.lastname: Zayed
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001774:Blood Chemical Analysis; D001835:Body Weight / Q000187:drug effects; D004576:Electromyography; D019570:Inhalation Exposure; D009043:Motor Activity / Q000187:drug effects; D009420:Nervous System / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*; D010095:Oxotremorine / Q000494:pharmacology; D010316:Particle Size; D051381:Rats; D014018:Tissue Distribution
chemical_list: D009942:Organometallic Compounds; D010095:Oxotremorine; C406182:manganese phosphate