Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans.
Using inverse PCR, two new genes (btrN and btrS) were identified upstream of the putative glycosyltransferase gene btrM in the butirosin-biosynthetic btr gene cluster of Bacillus circulans. The upstream gene btrS showed significant homology with stsC of Streptomyces griseus, which encodes L-glutamine:scyllo-inosose aminotransferase in the biosynthesis of streptomycin. The function of BtrS was further confirmed by heterologous expression in Escherichia coli and chemical identification of the conversion of 2-deoxy-scyllo-inosose into 2-deoxy-scyllo-inosamine. The identification of BtrS as L-glutamine:2-deoxy-scyllo-inosose aminotransferase is the first report of the aminotransferase gene responsible for 2-deoxystreptamine biosynthesis.
Tamegai
Hideyuki
H
Department of Chemistry, Tokyo Institute of Technology, Japan.
Nango
Eriko
E
Kuwahara
Mieko
M
Yamamoto
Hideki
H
Ota
Yasumasa
Y
Kuriki
Hisako
H
Eguchi
Tadashi
T
Kakinuma
Katsumi
K
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002076:Butirosin Sulfate / Q000096:biosynthesis*; D005798:Genes, Bacterial; D005973:Glutamine / Q000378:metabolism*; D006595:Hexosamines / Q000096:biosynthesis; D007294:Inositol / Q000031:analogs & derivatives* / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D000637:Transaminases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D006595:Hexosamines; D005973:Glutamine; D007294:Inositol; D002076:Butirosin Sulfate; C075548:2-deoxyinosose; D000637:Transaminases; C007265:2-deoxystreptamine