Screening of single nucleotide polymorphisms in nasopharyngeal carcinoma associated genes by denaturing high-performance liquid chromatography.
OBJECTIVE
To screen single nucleotide polymorphisms (SNPs) of 4 human genes at 6p21.3 by way of denaturing high-performance liquid chromatography (DHPLC).


METHODS
Four exons and 1 intron fragments in the PPP1R11, PPP1R10, FLOT1 and KIAA0170 genes at 6p21.3 isolated from the blood samples from 30 patients with nasopharyngeal carcinoma (NPC) and 28 healthy subjects were amplified by PCR, and the products analyzed by DHPLC. Some fragments of interest were sequenced and compared with the sequences available in National Center for Biotechnology Information (NCBI) database.


RESULTS
In the 5 fragments, 4 new SNPs were identified and 4 known SNP loci and genotypes were confirmed.


CONCLUSIONS
DHPLC is an effective, economical, and simple method with reliability for SNPs screening.
Huang
Hua
H
Institute of Oncology, First Military Medical University, Guangzhou 510515, China.
Zhou
Yu-Feng
YF
Jiang
Pei-Zhou
PZ
Shen
Xin-Ming
XM
Yao
Kai-Tai
KT
D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D000368:Aged; D018797:Cell Cycle Proteins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002874:Chromosome Mapping; D004273:DNA, Neoplasm / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C413163:MDC1 protein, human; D008565:Membrane Proteins; D009687:Nuclear Proteins; D015534:Trans-Activators; C106354:flotillins