Gastric protective effect of peripheral PYY through PYY preferring receptors in anesthetized rats.
The influence of intravenous peptide YY (PYY) on the gastric injury induced by 45% ethanol was investigated in urethane-anesthetized rats. PYY (25, 75, 125, and 250 pmol x kg(-1) x h(-1)) significantly reduced gastric lesions by 36, 59, 40, and 38%, respectively. Antibody against ratPYY (2 mg/rat) injected intravenously completely prevented the gastroprotective effect of intravenous PYY (75 pmol x kg(-1) x h(-1)), whereas injected intracisternally (460 microg/20 microl), it significantly prevented intracisternal PYY (24 pmol/rat)-induced 58% reduction of ethanol lesions but not that induced by intravenous PYY. Vagotomy did not influence the gastroprotective effect of intravenous PYY. The Y(1)/"PYY-preferring" receptor agonist [Pro(34)]PYY (75 pmol x kg(-1) x h(-1) iv) significantly decreased ethanol-induced gastric lesions by 82%, whereas [Leu(31), Pro(34)]NPY, a Y(1)/Y(3) agonist, and PYY-(3-36), a Y(2) agonist, had no effect. These data indicate that PYY-infused intravenously at doses reported to mimic postprandial peak blood levels prevents ethanol-induced gastric injury through vagal independent pathways and PYY-preferring receptors.
Kawakubo
Keishi
K
CURE: Digestive Diseases Research Center, Veteran's Affairs Greater Los Angeles Healthcare System, Department of Medicine, and Brain Research Institute, University of California Los Angeles, Los Angeles, California 90073, USA.
Yang
Hong
H
Taché
Yvette
Y
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000494:pharmacology; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology; D005753:Gastric Mucosa / Q000187:drug effects / Q000473:pathology; D007262:Infusions, Intravenous; D007276:Injections, Intraventricular; D007441:Intubation, Gastrointestinal; D008297:Male; D009478:Neuropeptide Y / Q000008:administration & dosage / Q000494:pharmacology; D019894:Peptide YY / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011964:Receptors, Gastrointestinal Hormone / Q000502:physiology*; D013272:Stomach Diseases / Q000517:prevention & control*; D013552:Swine; D014628:Vagotomy
D000906:Antibodies; D009478:Neuropeptide Y; D011964:Receptors, Gastrointestinal Hormone; C082172:peptide YY receptor; D019894:Peptide YY; D000431:Ethanol