title: Acute-phase protein gene expression in rat liver following whole body X-irradiation or partial hepatectomy.
abstract: We studied the effect of total body X-irradiation and partial hepatectomy on the acute phase protein gene expression in rat liver. Male rats of AO strain were irradiated with high X-ray doses, without any visible tissue damage. In contrast, partial hepatectomy consisted of surgical removal of 40% liver tissue. The changes in liver mRNA concentrations for positive acute-phase reactants including cysteine protease inhibitor, alpha(1)-acid glycoprotein, fibrinogen and haptoglobin, and albumin as a negative reactant were monitored by Northern blot and slot-blot hybridizations using corresponding [32P]dCTP labeled cDNA probes. While in the first 24 h after the partial hepatectomy, liver mRNA levels for the positive acute-phase reactants increased, briefly followed by an immediate decrease, the duration and timing of the acute-phase responses to the whole body X-irradiation were slightly different and lasted for as long as 72 h. Although both treatments induced the mRNA expression of acute-phase reactants in rat liver, the observed variations in the duration and intensity of the changes in mRNA levels for the acute-phase proteins in these two types of tissue damage suggest the involvement of specific mechanisms in a fine tuning of the non-specific acute-phase responses to meet the unique requirements of the particular injury.
authors.affiliation: Department of Medical Chemistry, Faculty of Medicine, University of Nis, Nis, Yugoslavia. trutic@medfak.medfak.ni.ac.yu
authors.forename: Natasa
authors.initials: N
authors.lastname: Trutic
authors.forename: Zvonko
authors.initials: Z
authors.lastname: Magic
authors.forename: Nadezda
authors.initials: N
authors.lastname: Urosevic
authors.forename: Koviljka
authors.initials: K
authors.lastname: Krtolica
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015870:Gene Expression / Q000528:radiation effects; D006498:Hepatectomy; D008099:Liver / Q000378:metabolism / Q000528:radiation effects*; D008115:Liver Regeneration; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism / Q000528:radiation effects; D051381:Rats; D011919:Rats, Inbred Strains; D014916:Whole-Body Irradiation / Q000009:adverse effects*
chemical_list: D000209:Acute-Phase Proteins; D012333:RNA, Messenger