Evaluation and applications of a new dye affinity adsorbent.
The basic properties of a new dye affinity adsorbent Toyopearl AF-Blue HC-650M and its applications to the purification of proteins were studied. The binding capacity for human serum albumin (HSA) was greater than 18 mg per ml gel. The dye leakage from Toyopearl AF-Blue HC-650M in 0.5 M NaOH and 0.5 M HCI was less compared with an agarose adsorbent. Caustic stability study also demonstrated this material withstood exposure to 0.1 M NaOH for 1 month with no significant loss of binding capacity for HSA. We purified human albumin from human serum and lactate dehydrogenase (LDH) from rabbit muscle extract in a single step. Sodium dodecylsulfate-polyacrylamide gel electrophoresis indicates that human albumin and LDH were highly purified.
Nakamura
Koji
K
Nan-yo Research Laboratory, Tosoh Corporation, Yamaguchi, Japan. k_naka@tosoh.co.jp
Suzuki
Takao
T
Kamichika
Toshito
T
Hasegawa
Masazumi
M
Kato
Yoshio
Y
Sasaki
Hiroo
H
Inouye
Kuniyo
K
D000327:Adsorption; D002846:Chromatography, Affinity / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D012709:Serum Albumin / Q000032:analysis*
D004396:Coloring Agents; D012709:Serum Albumin; D007770:L-Lactate Dehydrogenase