Expression of fibroblast growth factors in rat dorsal root ganglion neurons and regulation after peripheral nerve injury.
Using cDNA array, we observed the expression of eight members of the fibroblast growth factor (FGF) family, FGF 2, 5, 7, 9, 10, 13 and 14, in rat lumbar 4 and 5 dorsal root ganglia (DRGs). Over a period of 28 days after sciatic nerve transection, the array signals for FGF 2 and 7 were significantly increased in the DRGs, while FGF 13 decreased. Using the reverse transcription polymerase chain reaction (RT-PCR), we confirmed the axotomy-induced changes in the expression of FGF 7 and 13. hybridization showed that FGF 13 was expressed in 60% of DRG neurons under normal circumstance. Seven days after axotomy the number of FGF 13-positive neurons was decreased to 18%, but partially recovered to 40% after 28 days. FGF 13 immunoreactivity was also decreased. These data indicate that FGFs are important for DRG neurons under normal circumstance and after nerve injury.
Li
Guo-Dong
GD
Laboratory of Sensory System, Institute of Neuroscience, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, 200031, China.
Wo
Yan
Y
Zhong
Mei-Fang
MF
Zhang
Fang-Xiong
FX
Bao
Lan
L
Lu
Ying-Jin
YJ
Huang
Yao-De
YD
Xiao
Hua-Sheng
HS
Zhang
Xu
X
D000818:Animals; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000235:genetics; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000378:metabolism; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000503:physiopathology; D015870:Gene Expression / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D009437:Neuralgia / Q000378:metabolism* / Q000503:physiopathology; D009475:Neurons, Afferent / Q000378:metabolism*; D059348:Peripheral Nerve Injuries; D010523:Peripheral Nervous System Diseases / Q000378:metabolism* / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000502:physiology; D012584:Sciatic Nerve / Q000293:injuries / Q000503:physiopathology / Q000601:surgery; D015854:Up-Regulation / Q000235:genetics*
D012333:RNA, Messenger; D005346:Fibroblast Growth Factors