PKC-dependent IL-6 production and inhibition of IL-8 production by PKC activation in normal human skin fibroblasts under extremely high hydrostatic pressure.
Normal human dermal fibroblasts were found to survive and to be active in producing interleukin (IL)-6 and IL-8 under extremely high hydrostatic pressure, up to 40 MPa (1 atm=0.101325 MPa=1.03323 kgf/cm(2)), for 20 min. An inhibitor of protein kinase C (PKC) reduced the amount of IL-6 production, whereas IL-8 production was increased following pressure application. The activation of PKC in response to exposure to the pressure stress was detected by using the PKC-specific probe Rim-1. These findings indicate that IL-6 production induced by hydrostatic pressure stresses was dependent on the PKC signaling pathway. In contrast, pressure-induced IL-8 production was inhibited by PKC activity.
Koyama
Sumihiro
S
Frontier Research System for Extremophiles, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. skoyama@jamstec.go.jp
Aizawa
Masuo
M
D020134:Catalytic Domain / Q000187:drug effects; D002478:Cells, Cultured / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D006874:Hydrostatic Pressure; D007211:Indoles / Q000494:pharmacology; D007375:Interleukin-1 / Q000032:analysis; D019409:Interleukin-15 / Q000032:analysis; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D008301:Maleimides / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D012235:Rhodamines / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D013347:Subcellular Fractions / Q000201:enzymology
D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D007211:Indoles; D007375:Interleukin-1; D019409:Interleukin-15; D015850:Interleukin-6; D016209:Interleukin-8; D008301:Maleimides; D012235:Rhodamines; C082337:rim 1; D011493:Protein Kinase C; C070515:bisindolylmaleimide I