[Inhibitory effects of endostatin on interleukin-6 and interleukin-8 release by human umbilical vein endothelial cells].
OBJECTIVE
To investigate the inhibitory effects exercised by endostatin on the production of interleukin-6 (IL-6) and IL-8 by human umbilical vein endothelial cells (HUVECs).


METHODS
(HUVECs were isolated and cultured in vitro with endostatin (treated group) or PBS (control group), and the supernatant was harvested from the primary culture medium daily for 9 consecutive days starting from the first day of culture, followed by centrifugation. IL-6 and IL-8 contents in the supernatant were measured using sandwich enzyme-linked immunosorbent assay (ELISA).


RESULTS
IL-6 and IL-8 were detected in the supernatant of the control cell culture, and their amounts increased as the cell culture was prolonged, reaching the peak levels on day 6 (2 979.32+/-19.65 pg/ml and 6 018.87+/-56.74 pg/ml, respectively). In the treated group, however, the amounts of IL-6 and IL-8 were significantly lower than the control levels (P<0.01).


CONCLUSIONS
Endostatin can inhibit the growth and proliferation of endothelial cells, reducing their biological activities.
Zhang
Lin
L
Department of Respiratory Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Luo
Li-Min
LM
Xia
Hu
H
Zhuang
Lie-Qian
LQ
Tong
Wan-Cheng
WC
Wen
Jin-Xu
JX
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D043169:Endostatins; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*
D020533:Angiogenesis Inhibitors; D043169:Endostatins; D015850:Interleukin-6; D016209:Interleukin-8; D010446:Peptide Fragments; D003094:Collagen