title: Anti-idiotypic antibody specific for an infectious bursal disease virus-neutralizing monoclonal antibody does not interfere with the virus infection.
abstract: Infectious bursal disease virus (IBDV) capsid protein, VP2, contains a hypervariable domain that is recognized by virus-neutralizing antibodies. The virus-neutralizing epitope is highly conformation-dependent and the domain is speculated to be involved in the virus-target cell interaction. In this study, a polyclonal anti-idiotypic antibody (anti-id) was generated by the sequential immunization of a rabbit with a virus-neutralizing monoclonal antibody GI-11 which recognizes the VP2 hypervariable domain. Although the anti-id, which mimics the conformational epitope in the VP2 hypervariable domain, was expected to inhibit the virus infection, the anti-id did not interfere with either the virus binding or the infection to the target cell.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan. yamaguti@cc.gifu-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: A
authors.initials: A
authors.lastname: Igusa
authors.forename: A
authors.initials: A
authors.lastname: Setiyono
authors.forename: H
authors.initials: H
authors.lastname: Fukushi
authors.forename: K
authors.initials: K
authors.lastname: Hirai
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D018175:Birnaviridae Infections / Q000517:prevention & control*; D007243:Infectious bursal disease virus / Q000276:immunology*; D009500:Neutralization Tests; D011817:Rabbits; D015678:Viral Structural Proteins / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins