Exposure to power frequency magnetic fields and X-rays induces GAP-43 gene expression in human glioma MO54 cells.
We investigated the distribution and expression of growth associated protein-43 (GAP-43) in human glioma cells (MO54) after exposure to a magnetic field (60 Hz, 5 mT), with or without initial X-ionizing radiation (2 Gy), by using immunocytochemistry and the reverse transcription polymerase chain reaction (RT-PCR). GAP-43 was present in the cytoplasm, accumulating in the perinuclear area. An increase in GAP-43 expression was observed with a peak at 10 h at the mRNA level and at 12 h at the protein level, after exposure to the magnetic field. The increased level of GAP-43 protein returned to a normal level within 24 h of exposure to a 5 mT magnetic field. The kinetic pattern of GAP-43 expression induced by X-ionizing radiation was very similar to that induced by the magnetic field. These results suggest that the stimulation of GAP-43 expression could occur by a similar mechanism following exposure to X-rays or magnetic fields. We have provided the first evidence that exposure to a 5 mT magnetic field can induce GAP-43 gene expression in human glioma MO54 cells.
Ding
Gui-Rong
GR
Department of Radiation Medicine, The Fourth Military Medical University, Xi'an, China.
Nakahara
Takehisa
T
Miyakoshi
Junji
J
D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D019922:GAP-43 Protein / Q000096:biosynthesis* / Q000235:genetics / Q000528:radiation effects*; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D005910:Glioma / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D012016:Reference Values; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D014965:X-Rays
D019922:GAP-43 Protein