Glucose microbiosensor based on alumina sol-gel matrix/electropolymerized composite membrane.
A procedure is described that provides co-immobilization of enzyme and bovine serum albumin (BSA) within an alumina sol-gel matrix and a polyphenol layer permselective for endogenous electroactive species. BSA has first been employed for the immobilization of glucose oxidase (GOx) on a Pt electrode in a sol-gel to produce a uniform, thin and compact film with enhanced enzyme activity. Electropolymerization of phenol was then employed to form an anti-interference and protective polyphenol film within the enzyme layer. In addition, a stability-reinforcing membrane derived from (3-aminopropyl)-trimethoxysilane was constructed by electrochemically-assisted crosslinking. This hybrid film outside the enzyme layer contributed both to the improved stability and to permselectivity. The resulting glucose sensor was characterized by a short response time (<10 s), high sensitivity (10.4 nA/mM mm(2)), low interference from endogenous electroactive species, and a working lifetime of at least 60 days.
Chen
Xiaohong
X
Department of Chemistry, University of Kansas, Malott Hall, Lawrence, KS 66045-0046, USA.
Hu
Yibai
Y
Wilson
George S
GS
D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001786:Blood Glucose / Q000032:analysis; D002417:Cattle; D020099:Coated Materials, Biocompatible; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005782:Gels / Q000737:chemistry*; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D008567:Membranes, Artificial; D008904:Miniaturization; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry
D001786:Blood Glucose; D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D005782:Gels; D008567:Membranes, Artificial; D011108:Polymers; D012710:Serum Albumin, Bovine; D005949:Glucose Oxidase; D005947:Glucose; D000537:Aluminum Oxide