Restriction analysis of PCR amplified nrDNA regions revealed intraspecific variation within populations of Fusarium culmorum.
Seventy-five isolates of Fusarium culmorum with diverse geographical origin and host were analyzed using restriction digestion of polymerase chain reaction amplified nuclear ribosomal DNA intergenic spacer (IGS) and 28S gene regions. The 28S gene was conserved and has produced identical restriction patterns, however, the IGS region was substantially variable. The isolates were divided into 29 unique IGS haplotypes. There was limited resolution between clustering of isolates and their origin and/or host. The variability was distributed largely equally at both macro- and micro-geographical scale. The phylogeographic distribution pattern suggests a seed-borne dispersal of F. culmorum.
Mishra
Prashant K
PK
School of Plant Sciences, The University of Reading, Whiteknights, Berks RG6 6AS, UK. pmishra@afhe.ualberta.ca
Fox
Roland T V
RT
Culham
Alastair
A
D004271:DNA, Fungal / Q000032:analysis; D021901:DNA, Intergenic / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis*; D005670:Fusarium / Q000145:classification / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012339:RNA, Ribosomal, 28S
D004271:DNA, Fungal; D021901:DNA, Intergenic; D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S