title: Potentiation of serum response factor activity by a family of myocardin-related transcription factors.
abstract: Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac and smooth muscle cells, MRTF-A and -B are expressed in numerous embryonic and adult tissues. In SRF-deficient embryonic stem cells, myocardin and MRTFs are unable to activate SRF-dependent reporter genes, confirming their dependence on SRF. Myocardin and MRTFs comprise a previously uncharacterized family of SRF cofactors with the potential to modulate SRF target genes in a wide range of tissues.
authors.affiliation: Department of Molecular Biology, University of Texas, Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas 75390-9148, USA.
authors.forename: Da-Zhi
authors.initials: DZ
authors.lastname: Wang
authors.forename: Shijie
authors.initials: S
authors.lastname: Li
authors.forename: Dirk
authors.initials: D
authors.lastname: Hockemeyer
authors.forename: Lillian
authors.initials: L
authors.lastname: Sutherland
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Wang
authors.forename: Gerhard
authors.initials: G
authors.lastname: Schratt
authors.forename: James A
authors.initials: JA
authors.lastname: Richardson
authors.forename: Alfred
authors.initials: A
authors.lastname: Nordheim
authors.forename: Eric N
authors.initials: EN
authors.lastname: Olson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D018507:Gene Expression Regulation, Developmental; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D026362:Serum Response Factor / Q000235:genetics* / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors; D014981:Xenopus; D000099251:Myocardin
chemical_list: D018076:DNA, Complementary; D005982:Glutathione Transferase; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D026362:Serum Response Factor; D015534:Trans-Activators; D014157:Transcription Factors; D000099251:Myocardin