title: Activation of peroxidase-catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine with poly(salicylic acid 5-aminodisulfide).
abstract: 5-Aminosalicylic acid (5-ASA) inhibited by a mixed mechanism the peroxidase catalyzed oxidation of tetramethylbenzidine (TMB) in 0.015 M phosphate-citrate buffer (pH 6.4) supplemented with 5% DMSO and 5% DMF. Poly(salicylic acid 5-aminodisulfide) (poly(SAADS)) in 0.01 M phosphate buffer (pH 6.2-7.4) supplemented with 5% DMSO and 5% DMF effectively activated the peroxidase-catalyzed oxidation of TMB. The activation was quantitatively characterized by coefficients alpha (M-1) determined at different pH values: alpha increased linearly with increase in pH up to the maximal value of 2.44*105 M-1 at pH 7.0. The activating effect of poly(SAADS) on the peroxidase-catalyzed oxidation of TMB is explained by the activator properties of polyelectrolyte, with its anionic form interacting with peroxidase sites responsible for the acid-base catalysis.
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, 220141, Belarus. metelitza@iboch.bas-net.by
authors.forename: D I
authors.initials: DI
authors.lastname: Metelitza
authors.forename: E I
authors.initials: EI
authors.lastname: Karasyova
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001560:Benzidines / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D004220:Disulfides / Q000737:chemistry* / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019804:Mesalamine / Q000737:chemistry / Q000494:pharmacology; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D012459:Salicylates / Q000737:chemistry* / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D000975:Antioxidants; D001560:Benzidines; D004220:Disulfides; D011108:Polymers; D012459:Salicylates; C473767:poly(salicylic acid 5-aminodislufide); C021758:3,3',5,5'-tetramethylbenzidine; D019804:Mesalamine; D006735:Horseradish Peroxidase