title: The effects of vitamin E succinate on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells.
abstract: OBJECTIVE
  To investigate the effects of vitamin E succinate (VES) on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells.
  
  
  METHODS
  After SGC-7901 cells were treated with VES at different doses (5,10,20 mg x L(-1)) at different time, reverse transcription-PCR technique was used to detect the level of c-jun mRNA; Western Blot was applied to measure the expression of c-jun protein.
  
  
  RESULTS
  After the cells were treated with VES at 20 mg x L(-1) for 3 h, the expression rapidly reached its maximum that was 3.5 times of UT control (P<0.01). The level of c-jun mRNA was also increased following treatment of VES for 6 h. However,the expression after treatment of VES at 5 mg x L(-1) for 24 h was 1.6 times compared with UT control (P<0.01). Western blot analysis showed that the level of c-jun protein was obviously elevated in VES-treated SGC-7901 cells at 20 mg x L(-1) for 3 h. The expression of c-jun protein was gradually increased after treatment of VES at 20 mg x L(-1) for 3, 6, 12 and 24 h, respectively, with an evident time-effect relationship.
  
  
  CONCLUSIONS
  The levels of c-jun mRNA and protein in VES-treated SGC-7901 cells were increased in a dose- and time-dependent manner; the expression of c-jun was prolonged by VES, indicating that c-jun is involved in VES-induced apoptosis in SGC-7901 cells.
authors.affiliation: Department of Nutrition and Food Hygiene, Public Health School, Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Kun
authors.initials: K
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.forename: Yu-Juan
authors.initials: YJ
authors.lastname: Shan
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Wu
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Yu
mesh_terms: D015153:Blotting, Western; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D013274:Stomach Neoplasms; D024505:Tocopherols; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D014810:Vitamin E; D024505:Tocopherols