Role of dynamic interactions in effective signal transfer for Gbeta stimulation of phospholipase C-beta 2.
Heterotrimeric G protein subunits regulate their effectors by protein-protein interactions. The regions involved in these direct interactions have either signal transfer or general binding functions (Buck, E., Li, J., Chen, Y., Weng, G., Scarlata, S., and Iyengar, R. (1999) Science 283, 1332-1335). Although key determinants of signal transfer regions for G protein subunits have been identified, the mechanisms of signal transfer are not fully understood. We have used a combinatorial peptide approach to analyze one Gbeta region, Gbeta86-105, involved in signal transfer to the effector phospholipase C (PLC)-beta2 to gain a more mechanistic understanding of Gbeta/PLC-beta2 signaling. Binding and functional studies with the combinatorial peptides on interaction with and stimulation/inhibition of phospholipase Cbeta2 indicate that binding affinity can be resolved from EC(50) for functional effects, such that peptides that have wild type binding affinities have 15- to 20-fold lower EC(50) values. Although more potent, these peptides display a much lower extent of maximal stimulation. These peptides synergize with Gbetagamma or peptides encoding the second Gbeta42-54 signal transfer region in maximally stimulating phospholipase C-beta2. Other combinatorial peptides from the Gbeta86-105 region that bind to PLC-beta2 by themselves submaximally stimulate and extensively inhibit Gbetagamma stimulation of PLC-beta2. The intrinsic stimulation function can be attributed to Arg-96 and Ser-97, the synergy function to Trp-99, and the binding affinity to Thr-87, Val-90, Pro-94, Arg-96, Ser-97, and Val-100. These results indicate that, even within signal transfer regions, residues involved in binding can be resolved from those involved in signal transfer and that signal transfer is likely to be achieved through dynamic rather than steady-state interactions.
Buck
Elizabeth
E
Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029, USA.
Schatz
Peter
P
Scarlata
Suzanne
S
Iyengar
Ravi
R
D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D019204:GTP-Binding Proteins / Q000378:metabolism* / Q000502:physiology*; D006801:Humans; D007313:Insecta; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry; D054799:Phospholipase C beta; D010957:Plasmids / Q000378:metabolism; D011392:Proline / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D012694:Serine / Q000737:chemistry; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence; D010738:Type C Phospholipases / Q000235:genetics / Q000378:metabolism*; D014633:Valine / Q000737:chemistry
D007527:Isoenzymes; D009841:Oligonucleotides; D019151:Peptide Library; D010455:Peptides; D012694:Serine; D001120:Arginine; D011392:Proline; D010738:Type C Phospholipases; C518036:PLCB2 protein, human; D054799:Phospholipase C beta; D019204:GTP-Binding Proteins; D014633:Valine