title: Molecular cloning and sequence analysis of heavy- and light-chain variable region genes of anti-CD71 monoclonal antibody.
abstract: OBJECTIVE
  To clone heavy-chain and light-chain variable region (VH and VL) gene of mouse-anti-human CD71 monoclonal antibody (mAb).
  
  
  METHODS
  One-step method was used to extract total RNA, and a set of oligonucleotide primers were designed to amplify the cDNAs with reverse transcriptase-polymerase chain reaction (RT-PCR), and the resultant products were respectively cloned into PMD18-T vector and their sequences analyzed.
  
  
  RESULTS
  The PCR product obtained with the oligonucleotide primers for the variable region of mouse immunoglobulin heavy chain was about 350 bp and that with oligonucleotide primers for the light chain was about 320 bp, and their DNA sequences were determined.
  
  
  CONCLUSIONS
  The length of the cloned heavy chain variable region was 348 bp, belonging to mouse heavy-chain subgroup II(A); the light-chain variable region was 336 bp that belongs to mouse kappa light-chain subgroup II.
authors.affiliation: Department of Immunology, Institute of Biological Products of Wuhan, Wuhan 430060, China.
authors.forename: Zhi-You
authors.initials: ZY
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Shen
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Bi
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Jin
authors.forename: Ke-Fang
authors.initials: KF
authors.lastname: Sun
authors.forename: Mo-Song
authors.initials: MS
authors.lastname: Yu
authors.forename: Liang-Ru
authors.initials: LR
authors.lastname: Shi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011990:Receptors, Transferrin; D017422:Sequence Analysis, DNA
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C068126:CD71 antigen; D018076:DNA, Complementary; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D011990:Receptors, Transferrin