Tech.Sight. Phage display. Affinity selection from biological libraries.
Phage display is a simple yet powerful technology that is used to rapidly characterize protein-protein interactions from amongst billions of candidates. This widely practiced technique is used to map antibody epitopes, create vaccines and to engineer peptides, antibodies and other proteins as both diagnostic tools and as human therapeutics. We overview the history of phage display and several recent applications.
Smothers
James F
JF
Department of Antibody Technologies, Amgen Inc., Seattle, WA 98101, USA. SmotherJ@amgen.com
Henikoff
Steven
S
Carter
Paul
P
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D000915:Antibody Affinity; D001435:Bacteriophages / Q000235:genetics; D030562:Databases, Protein; D018604:Epitope Mapping; D006801:Humans; D036103:Nanotechnology; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012666:Semiconductors
D000906:Antibodies; D019151:Peptide Library; D010455:Peptides; D011506:Proteins; D011993:Recombinant Fusion Proteins