[Observation of the microstructure and ultrastructure of microvessels induced by OS-732 cell line].
OBJECTIVE
To observe the characteristics of the microstructure and ultrastructure of the microvessels induced by human osteosarcoma cell line (OS-732).


METHODS
OS-732 cells were inoculated into the chick embryo chorioallantoic membrane to induce the growth of microvessels, the microstructure and ultrastructure of which were observed using dissecting microscope, light microscope and transmission electron microscope.


RESULTS
OS-732 cell line was strongly angiogenic, and newly generated capillaries converging upon the tumor were visualized under dissecting microscope. Observation with transmission electron microscope found that the wall of the microvessels deficient in elements of smooth muscles was consisted of a monolayer of vascular endothelial cells with widened gaps in between, and the basement membrane appeared irregular.


CONCLUSIONS
The microvessels induced by tumors have pathological and physiopathological characteristics consistent with the tumors, and setting the microvessels as a therapeutic target against osteosarcoma may help improve the prognosis of patients with this disease.
Li
Ying-Jia
YJ
Departments of Ultrasonography, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Wang
Dong
D
Zhou
Ya
Y
D000818:Animals; D002642:Chick Embryo; D006801:Humans; D008833:Microcirculation / Q000503:physiopathology; D008854:Microscopy, Electron; D009389:Neovascularization, Pathologic / Q000473:pathology / Q000503:physiopathology*; D012516:Osteosarcoma / Q000473:pathology* / Q000503:physiopathology; D014407:Tumor Cells, Cultured