Copper accumulation in actinomyces druses during endometritis after long-term use of an intrauterine contraceptive device.
A 32-year-old woman carried a copper intrauterine contraceptive device (IUCD) or intrauterine pessar (IAP) for more than 5 years. She had acyclic menstrual bleedings and underwent a corpus abrasio after explantation of IUCD. The histological study of paraffin sections showed an actinomycotic endometritis with brown to black deposits in or around typical actinomyces druses, but there was no carcinoma. The electron microscopic study of these accumulations by electron energy loss spectroscopy (EELS) in TEM demonstrated copper deposits in the shell and matrix of these druses as well as inside the bacteria. With scanning electron microscopy (SEM) and Energy Dispersive X-Ray Analysis (EDX), the electron-dense accumulations revealed high signals for copper and sulfur, but also of phosphorus and oxygen in a lower extent. This copper accumulation is discussed as an active uptake and concentration by these actinomyces bacteria.
Jonas
Ludwig
L
Electron Microscopic Center, in the Department of Pathology, University of Rostock, Germany. ludwig.jonas@med.uni-rostock.de
Baguhl
Frank
F
Wilken
Hans-Peter
HP
Haas
Hermann Josef
HJ
Nizze
Horst
H
D000190:Actinomyces / Q000378:metabolism* / Q000648:ultrastructure; D000196:Actinomycosis / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D003300:Copper / Q000032:analysis / Q000378:metabolism*; D004577:Electron Probe Microanalysis; D004716:Endometritis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007435:Intrauterine Devices, Copper; D008855:Microscopy, Electron, Scanning; D011247:Pregnancy
D003300:Copper