title: Immobilized enzymes as catalytically-active tools for nanofabrication.
abstract: One efficient strategy for creating nanostructures on surfaces is to use the catalytic properties of a surface molecule. This strategy benefits from the amplification and chemical specificity inherent in catalysis. We describe a demonstration of the key step of such a strategy: the surface trapping of a product generated by a nanometer-scale patch of surface-bound enzyme. Nanografting was used to create a approximately 70-nm patch of carboxylic acid groups surrounded by antibiofouling oligio(ethyleneoxide) groups on the surface of a gold ball. A catalytic site was prepared by immobilization of acetylcholine esterase to the carboxylic acid patch, and a product trap was prepared by scratching a small hole in the antibiofouling surface to reveal the gold surface. Two hours after addition of acetylthiocholine, the trap was filled. This demonstrated that the enzyme had catalyzed a reaction and that the product had been used to modify the surface film.
authors.affiliation: Department of Chemistry, Virginia Tech, Blacksburg, Virginia 24061-0212, USA.
authors.forename: Chang-Hyun
authors.initials: CH
authors.lastname: Jang
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Stevens
authors.forename: Paul R
authors.initials: PR
authors.lastname: Carlier
authors.forename: Michael A
authors.initials: MA
authors.lastname: Calter
authors.forename: William A
authors.initials: WA
authors.lastname: Ducker
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D000122:Acetylthiocholine / Q000737:chemistry; D000818:Animals; D002384:Catalysis; D004593:Electrophorus; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*
chemical_list: D004800:Enzymes, Immobilized; D000122:Acetylthiocholine; D006046:Gold; D000110:Acetylcholinesterase