Identification of Echinococcus granulosus eggs.
The eggs from Echinococcus granulosus contaminate the environment spreading out the disease among the herbivorous. The differential diagnosis of the embriophores recovered from the soil is very difficult by morphologic and immunologic methods. In this paper we evaluate the EgO/DNA-IM1 for identification of E. granulosus oncosphere DNA and differentiation of eggs from other Taeniid. The positive result of the PCR technique shows an amplification fragment of the expected size (285 bp) corresponding to the partial sequence of the mitochondrial gene of the cytochrome oxidase CO1 from E. granulosus (391 bp). The fragment is not present in the DNA from Echinococcus multilocularis, Taenia hydatigena, Taenia saginata, Diphyll-obothrium latum, and Hymenolepis nana. It could be useful to rule out Taenia taeniformis, Taenia solium, Taenia pisiformis, and Taenia crassiceps, which sequences do not belong to the primer. We concluded that the PCR amplification employing the EgO/DNA-IM1 primer set showed high sensitivity and specificity for the identification of Echinococcus granulosus eggs.
Cabrera
Marta
M
Departamento de Parasitologi;a. Instituto Nacional de Enfermedades Infecciosas "Dr Carlos G Malbrán," Av Vélez Sársfield 563 (1281), Buenos Aires, Argentina.
Canova
Sergio
S
Rosenzvit
Mara
M
Guarnera
Eduardo
E
D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001483:Base Sequence; D015342:DNA Probes; D018509:DNA, Helminth / Q000032:analysis; D004285:Dogs; D004443:Echinococcosis / Q000175:diagnosis* / Q000453:epidemiology; D004446:Echinococcus / Q000302:isolation & purification*; D019353:Endemic Diseases; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D015342:DNA Probes; D018509:DNA, Helminth