E-selectin-inducing activity in plasma from type 2 diabetic patients with maculopathy.
Diabetic maculopathy (DMa) is a leading cause of visual loss in the western world. We examined whether plasma from type 2 diabetic patients with DMa contains factor(s) capable of inducing expression of the adhesion molecules E-selectin and VCAM-1 or cellular proliferation in cultured endothelial cells. Four gender-, age-, and duration (diabetes groups)-matched groups of 20 subjects each participated: 1) subjects with normal glucose tolerance (NGT), 2) subjects with impaired glucose tolerance (IGT), 3) type 2 diabetic patients without retinopathy, and 4) type 2 diabetic patients with DMa. Fasting plasma was added to in vitro-grown human umbilical vein endothelial cells for 6 h, after which E-selectin and VCAM-1 expression was measured. Proliferation was evaluated by thymidine incorporation. The individuals were characterized by measurement of 24-h ambulatory blood pressure, urinary albumin excretion rate, Hb A(1c), and blood lipids. Plasma from type 2 diabetic patients with DMa induced a significantly higher expression of E-selectin in endothelial cells than did plasma from subjects with NGT (259 +/- 23 x 10(3) vs. 198 +/- 19 x 10(3); arbitrary absorbance units; P < 0.05). There were no significant differences in plasma stimulatory effects on VCAM-1 expression or on thymidine incorporation between groups. These findings suggest that plasma from type 2 diabetic patients with DMa contains factor(s) capable of inducing the expression of E-selectin in endothelial cells. Enhanced expression of E-selectin may contribute to the development of DMa in type 2 diabetes.
Knudsen
S T
ST
Medical Department M (Diabetes & Endocrinology), Institute of Pathology, Aarhus University Hospital, Denmark. stk@dadlnet.dk
Foss
C H
CH
Poulsen
P L
PL
Bek
T
T
Ledet
T
T
Mogensen
C E
CE
Rasmussen
L M
LM
D000419:Albuminuria; D001769:Blood; D001794:Blood Pressure; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D003930:Diabetic Retinopathy / Q000097:blood*; D019040:E-Selectin / Q000032:analysis / Q000235:genetics*; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D018149:Glucose Intolerance / Q000097:blood; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D014471:Umbilical Veins; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis / Q000235:genetics
D004247:DNA; D019040:E-Selectin; D006442:Glycated Hemoglobin; D008055:Lipids; D019010:Vascular Cell Adhesion Molecule-1