title: Establishment of clonal colony-forming assay system for pancreatic stem/progenitor cells.
abstract: Pluripotent stem cells found in a number of organs are usually in small cell populations. However, under adaptive stimulation, they enter the stage of growth and differentiation to compensate for the loss of differentiated cells. To analyze stem cell potential precisely, the exclusion of other differentiated cells and a clonal assay system are strongly required. In this study, we established a colony-forming assay system for pancreatic stem/progenitor cells in vitro. In this culture condition, they received signals for growth and differentiation, and formed clonal colonies including pancreatic endocrine-lineage cells, such as alpha and beta cells. By combining this culture system with flow cytometric cell sorting, pancreatic stem/progenitor cells will be enriched, and their potential can be analyzed precisely in single cell-based experiments.
authors.affiliation: Department of Surgery, Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suzuki
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Oyama
authors.forename: Katashi
authors.initials: K
authors.lastname: Fukao
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Nakauchi
authors.forename: Hideki
authors.initials: H
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells; D003114:Colony-Forming Units Assay / Q000379:methods*; D005934:Glucagon / Q000302:isolation & purification; D007328:Insulin; D007515:Islets of Langerhans / Q000254:growth & development; D051379:Mice; D010179:Pancreas / Q000166:cytology*; D052336:Proglucagon; D011384:Proinsulin / Q000302:isolation & purification; D011498:Protein Precursors / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology
chemical_list: D007328:Insulin; D011498:Protein Precursors; D052336:Proglucagon; C013737:preproinsulin; D005934:Glucagon; D011384:Proinsulin