Protective effect of a lignan-containing flaxseed extract against CCl(4)-induced hepatic injury.
Carbon tetrachloride (CCl(4)) -induced hepatotoxicity is a commonly used model for investigating lipid peroxidation-related tissue injury. In the present study, the effect of flaxseed extract was observed on histological sections, glutathione-content and DNA strand breaks. Lignan-containing flaxseed extract (1.6 g/kg body weight/day) was daily administered with intragastric injection to rats for three days, on the fourth day, CCl(4) (2 g/kg) was intraperitoneally injected. Liver tissue was sampled at 24 hr after administering CCl(4). Liver-necrosis was observed in CCl(4)-injected rats without pretreatment of flaxseed extract. Pretreatment of flaxseed extract reduced extent of the necrosis found 24 hr after the intraperitoneal administration of CCl(4). Pretreatment of flaxseed extract protect against CCl(4)-induced decrease of reduced glutathione-content measured from reactions with 5,5'-dithiobis-(2-nitrobenzoic acid) and also protect against the elevation of DNA strand breaks in the liver cells measured by comet assay. Flaxseed-extract appears to protect liver cells against CCl(4)-induced necrosis.
Endoh
Daiji
D
Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
Okui
Toyo
T
Ozawa
Shuji
S
Yamato
Osamu
O
Kon
Yasuhiro
Y
Arikawa
Jiro
J
Hayashi
Masanobu
M
D000818:Animals; D002251:Carbon Tetrachloride / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury; D004249:DNA Damage / Q000187:drug effects; D004334:Drug Administration Schedule; D019597:Flax / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D017705:Lignans / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008107:Liver Diseases / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D009336:Necrosis; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D017705:Lignans; D010936:Plant Extracts; D002251:Carbon Tetrachloride; D005978:Glutathione