Enhancement of gene transfer to cervical cancer cells using transferrin-conjugated liposome.
Transferrin-conjugated cationic liposome (Tf-DDAB liposome) was developed as a targeted gene delivery system by using heterobifunctional cross-linking agent, N-succimidyl-3-(2-pyridyldithio) propionate (SPDP), and gradient metrizamide ultracentrifugation method. Physico-chemical properties of Tf-liposome were determined by scanning/transmission electron microscopy (SEM/TEM) and dynamic laser-light scattering method (DLS) with a mean diameter of 584 +/- 15 nm. Gel retardation assay was performed using various DDAB:DNA ratios, and proved that the 6:1 weight ratio formulation gave the most neutralized complex. In vitro transfection was done in human cervical cancer cells, HeLa, and the transfection efficiency of Tf-liposome was found to be fivefold higher than that of unconjugated (plain) DDAB liposome and twofold higher than that of Lipofectin. In conclusion, a target-oriented Tf-DDAB liposome was made successfully and proved to be very efficient in DNA delivery into the cervical cancer cells in culture.
Joo
Soo-Yeon
SY
College of Pharmacy, Sookmyung Women's University, Chungpa-Dong 2-Ka, Yongsan-Ku, Seoul 140-742, Korea.
Kim
Jin-Seok
JS
D003432:Cross-Linking Reagents; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes; D008854:Microscopy, Electron; D013388:Succinimides; D014162:Transfection; D014168:Transferrin / Q000737:chemistry* / Q000378:metabolism; D002583:Uterine Cervical Neoplasms; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D003432:Cross-Linking Reagents; D008081:Liposomes; D013388:Succinimides; D014168:Transferrin; C018151:N-succinimidyl 3-(2-pyridyldithio)propionate; D001616:beta-Galactosidase