Infiltrating eosinophils and eotaxin: their association with idiopathic eosinophilic esophagitis.
BACKGROUND
Idiopathic eosinophilic esophagitis (IEE) is a very rare disease characterized by thickening and eosinophil infiltration of the esophagus. The most potent chemotactic factor for eosinophils is eotaxin, and its pathophysiologic significance in IEE needs to be elucidated.


OBJECTIVE
To study the association between eotaxin and IEE.


METHODS
We examined eotaxin expression in the esophagus of an IEE patient in comparison to controls by immunohistochemistry using a monoclonal antibody for human eotaxin. We also measured the free eotaxin level and the total (free and bound-form) eotaxin level in blood by enzyme-linked immunosorbent assay before and after the initiation of steroid therapy.


RESULTS
Most of the infiltrating eosinophils in the affected esophageal tissue showed immunohistochemical staining with anti-eotaxin antibody. In blood samples, the free eotaxin level was slightly elevated before treatment, whereas the total eotaxin level was within the normal range. Unexpectedly, the total eotaxin level increased dramatically after the initiation of steroid therapy, whereas the increase of free eotaxin was modest.


CONCLUSIONS
Infiltrating eosinophils that express eotaxin and the changes of blood eotaxin levels during steroid therapy suggest that eotaxin may be associated with IEE.
Fujiwara
Hiroshi
H
Department of Molecular Medicine, Osaka University Graduate School of Medicine, Suita, Japan. fujiwara@qc5.so-net.ne.jp
Morita
Atsushi
A
Kobayashi
Hideyuki
H
Hamano
Katsura
K
Fujiwara
Yoshiyuki
Y
Hirai
Koichi
K
Yano
Masahiko
M
Naka
Tetsuji
T
Saeki
Yukihiko
Y
D054413:Chemokine CCL11; D019742:Chemokines, CC / Q000097:blood*; D002631:Chemotactic Factors, Eosinophil / Q000378:metabolism; D004802:Eosinophilia / Q000276:immunology*; D004804:Eosinophils / Q000502:physiology*; D004941:Esophagitis / Q000276:immunology / Q000503:physiopathology*; D004947:Esophagus / Q000276:immunology; D005260:Female; D006801:Humans; D008875:Middle Aged
C515456:CCL11 protein, human; D054413:Chemokine CCL11; D019742:Chemokines, CC; D002631:Chemotactic Factors, Eosinophil