title: Nogo-A expressed in Schwann cells impairs axonal regeneration after peripheral nerve injury.
abstract: Injured axons in mammalian peripheral nerves often regenerate successfully over long distances, in contrast to axons in the brain and spinal cord (CNS). Neurite growth-inhibitory proteins, including the recently cloned membrane protein Nogo-A, are enriched in the CNS, in particular in myelin. Nogo-A is not detectable in peripheral nerve myelin. Using regulated transgenic expression of Nogo-A in peripheral nerve Schwann cells, we show that axonal regeneration and functional recovery are impaired after a sciatic nerve crush. Nogo-A thus overrides the growth-permissive and -promoting effects of the lesioned peripheral nerve, demonstrating its in vivo potency as an inhibitor of axonal regeneration.
authors.affiliation: Brain Research Institute, University of Zurich, and Department of Biology, Swiss Federal Institute of Technology Zurich, CH-8057 Zurich, Switzerland. caroline.pot@access.unizh.ch
authors.forename: Caroline
authors.initials: C
authors.lastname: Pot
authors.forename: Marjo
authors.initials: M
authors.lastname: Simonen
authors.forename: Oliver
authors.initials: O
authors.lastname: Weinmann
authors.forename: Lisa
authors.initials: L
authors.lastname: Schnell
authors.forename: Franziska
authors.initials: F
authors.lastname: Christ
authors.forename: Sascha
authors.initials: S
authors.lastname: Stoeckle
authors.forename: Philipp
authors.initials: P
authors.lastname: Berger
authors.forename: Thomas
authors.initials: T
authors.lastname: Rülicke
authors.forename: Ueli
authors.initials: U
authors.lastname: Suter
authors.forename: Martin E
authors.initials: ME
authors.lastname: Schwab
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D019922:GAP-43 Protein / Q000378:metabolism; D005786:Gene Expression Regulation; D006131:Growth Inhibitors / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009185:Myelin Proteins / Q000235:genetics / Q000378:metabolism*; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000502:physiology*; D000070798:Nogo Proteins; D051381:Rats; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000293:injuries / Q000502:physiology* / Q000648:ultrastructure; D019076:Transgenes
chemical_list: D019922:GAP-43 Protein; D006131:Growth Inhibitors; D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603199:RTN4 protein, human; C000603201:Rtn4 protein, mouse; C000603197:Rtn4 protein, rat