Cloning of a grapevine Botrytis-responsive gene that has homology to the tobacco hypersensitivity-related hsr203J.
A cDNA encoding a putative HSR203J-like protein (BIG8.1) was obtained from total RNA isolated from Botrytis cinerea-infected grapevine leaves using differential display and RACE techniques. Real time RT-PCR analysis confirmed that the level of mRNA corresponding to BIG8.1 increased in grapevine leaves during the infection progress by B. cinerea. No significant change in mRNA level was observed in leaves after UV exposure. This expression pattern suggests that BIG8.1 could be a HR-specific marker in grapevine like hsr203J in tobacco.
Bézier
Annie
A
Laboratoire de Biologie et Physiologie Végétales, Université de Reims Champagne-Ardenne, Equipe de Biochimie et Biologie Moléculaire des Plantes, URVVC-EA 2069, UFR Sciences, Moulin de la Housse-BP 1039, F-51687 Reims Cedex 2, France.
Lambert
Bernard
B
Baillieul
Fabienne
F
D000595:Amino Acid Sequence; D020171:Botrytis / Q000254:growth & development*; D003001:Cloning, Molecular; D004950:Esterases / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics*; D014466:Ultraviolet Rays; D027843:Vitis / Q000235:genetics* / Q000382:microbiology
D010940:Plant Proteins; D012333:RNA, Messenger; D004950:Esterases; C088007:hsr203J protein, Nicotiana tabacum