title: Remarkable increase in the concentration of 8-hydroxyguanosine in cerebrospinal fluid from patients with Alzheimer's disease.
abstract: To investigate the possible role of oxidative RNA damage in the pathogenesis of Alzheimer's disease (AD), the concentrations of the oxidative stress marker 8-hydroxyguanosine (8-OHG) were measured in the cerebrospinal fluid (CSF) and the serum of patients with AD and control subjects. The concentration of 8-OHG in CSF in AD patients was approximately fivefold that in controls (P < 0.001). The concentration of 8-OHG in CSF decreased significantly with the duration of illness (r(s) = -0.48, P < 0.05) and the progression of cognitive dysfunctions (r(s) = 0.67, P < 0.01). However, the concentration of 8-OHG in CSF showed no correlation with that in serum in both the controls and AD patients. In addition, the concentration of 8-OHG in serum was not significantly altered in AD patients compared to that in controls, suggesting that the 8-OHG concentrations in the CSF do not reflect those in serum and may be probably reflect those in brain tissue. These in vivo findings suggest a possible role of 8-OHG and increased oxidative RNA damage in the early stage of the development of AD.
authors.affiliation: Department of Neurology, Iwate Medical University, Morioka, Iwate, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Abe
authors.forename: Hideo
authors.initials: H
authors.lastname: Tohgi
authors.forename: Chiaki
authors.initials: C
authors.lastname: Isobe
authors.forename: Takahiko
authors.initials: T
authors.lastname: Murata
authors.forename: Chigumi
authors.initials: C
authors.lastname: Sato
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000097:blood / Q000134:cerebrospinal fluid* / Q000235:genetics; D001921:Brain / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D006151:Guanosine / Q000031:analogs & derivatives* / Q000097:blood / Q000134:cerebrospinal fluid*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics*; D012313:RNA / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D006151:Guanosine; C046215:8-hydroxyguanosine; D012313:RNA