title: Responses of IgM for enterovirus 71 infection.
abstract: A rapid serological assay was developed for detection of specific IgM to enterovirus 71, a human picornavirus that is usually associated with severe central nervous system complications. The sensitivity and specificity of this "in-house" mu-capture enzyme linked-immunosorbent assay was assessed by testing 213 serum samples. With the conventional virus culture as a standard method, the sensitivity and specificity were 91.5 and 93.1%, respectively, for this newly developed immunoassay. This method allows for detection of the IgM responses from the patients either infected by genotype B or genotype C of enterovirus 71. IgM can be detected as early as the second day from the onset of disease. IgM responses exhibit 100% positive rate from enterovirus 71-infected patients with complications, including encephalitis, meningitis, polio-like syndrome, pulmonary edema, and fatal cases. These findings suggest that detection of specific IgM by the use of enzyme linked-immunosorbent assay is a rapid and valuable way for the diagnosis of enterovirus 71 infection.
authors.affiliation: School of Medical Technology, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Kuo-Chien
authors.initials: KC
authors.lastname: Tsao
authors.forename: Err-Cheng
authors.initials: EC
authors.lastname: Chan
authors.forename: Luan-Yin
authors.initials: LY
authors.lastname: Chang
authors.forename: Pi-Yueh
authors.initials: PY
authors.lastname: Chang
authors.forename: Chung-Guei
authors.initials: CG
authors.lastname: Huang
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Chen
authors.forename: Shih-Cheng
authors.initials: SC
authors.lastname: Chang
authors.forename: Tzou-Yien
authors.initials: TY
authors.lastname: Lin
authors.forename: Chien-Fen
authors.initials: CF
authors.lastname: Sun
authors.forename: Shin-Ru
authors.initials: SR
authors.lastname: Shih
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D004770:Enterovirus / Q000276:immunology / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D012680:Sensitivity and Specificity; D012703:Serotyping
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M