New Fe(III)Zn(II) complex containing a single terminal Fe-O(phenolate) bond as a structural and functional model for the active site of red kidney bean purple acid phosphatase.
The new heterodinuclear complex [Fe(III)Zn(II)(BPBPMP)(OAc)(2)]ClO(4) (1) with the unsymmetrical N(5)O(2) donor ligand 2-bis[((2-pyridylmethyl)-aminomethyl)-6-[(2-hydroxybenzyl)(2-pyridylmethyl)]-aminomethyl]-4-methylphenol (H(2)BPBPMP) has been synthesized and characterized by X-ray crystallography, which reveals that the complex cation has an Fe(III)Zn(II)(mu-phenoxo)-bis(mu-carboxylato) core. Solution studies of 1 indicate that a pH-induced change of the bridging acetate occurs, and the formation of an active [(OH)Fe(III)Zn(II)(OH(2))] species as a highly efficient catalyst under weakly acidic conditions for phosphate diesters hydrolysis is proposed.
Lanznaster
Mauricio
M
Departamento de Química-LABINC-Universidade Federal de Santa Catarina, Campus Trindade, BR-88040-900 Florianópolis-SC, Brazil.
Neves
Ademir
A
Bortoluzzi
Adailton J
AJ
Szpoganicz
Bruno
B
Schwingel
Erineu
E
D000135:Acid Phosphatase / Q000737:chemistry*; D018360:Crystallography, X-Ray; D007887:Fabaceae / Q000201:enzymology*; D005296:Ferrous Compounds / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D062385:Hydroxybenzoates / Q000737:chemistry*; D007202:Indicators and Reagents; D007700:Kinetics; D000432:Methanol; D008958:Models, Molecular; D011487:Protein Conformation; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D015032:Zinc / Q000737:chemistry*
D005296:Ferrous Compounds; D062385:Hydroxybenzoates; D007202:Indicators and Reagents; D012997:Solvents; D000135:Acid Phosphatase; C017616:phenolic acid; D015032:Zinc; D000432:Methanol