Efferent projections from the striatal patch compartment: anterograde degeneration after selective ablation of neurons expressing mu-opioid receptor in rats.
Local injection of mu-opioid receptor specific neurotoxin, dermorphin-saporin, into the striatum resulted in selective degeneration of striatal neurons in the patch compartment. We analyzed subsequent anterograde degeneration of axons and terminals at light and electron microscopic level. Light microscopic examination after silver impregnation method revealed that degenerating axons and terminals arising from the striatal patch compartment were distributed in the globus pallidus, entopeduncular nucleus, and substantia nigra. They were found in both pars reticulata and compacta of the substantia nigra. Electron microscopic examination revealed that the degenerating axon terminals contained large pleomorphic vesicles and formed symmetric synapses on dendrites. The present results suggest that patch neurons expressing mu-opioid receptor send projection fibers to multiple nuclei of the basal ganglia.
Tokuno
Hironobu
H
Department of Brain Structure, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Tokyo 183-8526, Japan. tokuno@tmin.ac.jp
Chiken
Satomi
S
Kametani
Kiyokazu
K
Moriizumi
Tetsuji
T
D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D004525:Efferent Pathways / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D005260:Female; D000922:Immunotoxins / Q000494:pharmacology; D008297:Male; D009699:N-Glycosyl Hydrolases; D009410:Nerve Degeneration / Q000378:metabolism* / Q000473:pathology*; D009842:Oligopeptides / Q000494:pharmacology; D018847:Opioid Peptides; D010940:Plant Proteins / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017450:Receptors, Opioid, mu / Q000096:biosynthesis* / Q000648:ultrastructure; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins
D000922:Immunotoxins; D009842:Oligopeptides; D018847:Opioid Peptides; D010940:Plant Proteins; D017450:Receptors, Opioid, mu; D054789:Ribosome Inactivating Proteins, Type 1; C030058:dermorphin; D009699:N-Glycosyl Hydrolases; D000076985:Saporins