Isolation and characterization of the immunoglobulin-binding protein from Yersinia pseudotuberculosis.
A high molecular weight immunoglobulin-binding protein localized on the surface of bacterial cells has been isolated from the protein fraction of the outer membrane of Yersinia pseudotuberculosis, and its properties are described. The immunoglobulin-binding protein is a trypsin-resistant and temperature-sensitive beta-structured protein. As shown by MALDI-TOF mass spectrometry, after heating at 100 degrees C the molecular weight of the protein constituted 37.5 kD. The native protein is capable of interacting with human and rabbit IgG but looses the ability to bind the immunoglobulins after the temperature denaturation. The immunoglobulin-binding protein binds to the Fc-fragments of the immunoglobulins and binding depends on the presence of calcium ions.
Naberezhnykh
G A
GA
Pacific Institute of Bioorganic Chemistry, Far East Division of the Russian Academy of Sciences, Vladivostok, 690022, Russia. naber@piboc.dvo.ru
Sidorin
E V
EV
Dmitrenok
P S
PS
Kim
N Yu
NY
Solov'eva
T F
TF
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods; D006358:Hot Temperature; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D008970:Molecular Weight; D011485:Protein Binding; D017433:Protein Structure, Secondary; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015011:Yersinia pseudotuberculosis / Q000276:immunology / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007136:Immunoglobulins; D016650:Fluorescein-5-isothiocyanate