title: Microfabricated 384-lane capillary array electrophoresis bioanalyzer for ultrahigh-throughput genetic analysis.
abstract: A microfabricated 384-lane capillary array electrophoresis device is developed and utilized for massively parallel genetic analysis. The 384 capillary lanes, arrayed radially about the center of a 200-mm-diameter glass substrate sandwich, are constructed using scalable microfabrication techniques derived from the semiconductor industry. Samples are loaded into reservoirs on the perimeter of the wafer, separated on the 8-cm-long poly(dimethylacrylamide) gel-filled channels, and detected with a four-color rotary confocal fluorescence scanner. The performance and throughput of this bioanalyzer are demonstrated by simultaneous genotyping 384 individuals for the common hemochromatosis-linked H63D mutation in the human HFE gene in only 325 s. This lab-on-a-chip device thoroughly exploits the power of microfabrication to produce high-density capillary electrophoresis arrays and to use them for high-throughput bioanalysis.
authors.affiliation: Department of Chemistry, University of California, Berkeley 94720, USA.
authors.forename: Charles A
authors.initials: CA
authors.lastname: Emrich
authors.forename: Huijun
authors.initials: H
authors.lastname: Tian
authors.forename: Igor L
authors.initials: IL
authors.lastname: Medintz
authors.forename: Richard A
authors.initials: RA
authors.lastname: Mathies
mesh_terms: D004247:DNA / Q000737:chemistry / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D005838:Genotype; D006801:Humans; D008838:Microcomputers; D008967:Molecular Biology / Q000295:instrumentation*; D036103:Nanotechnology
chemical_list: D004247:DNA