title: PACAP enhances the expression of CD11b, CD66b and CD63 in human neutrophils.
abstract: Pituitary adenylate cyclase-activating peptide (PACAP) is a neuropeptide with strong bronchodilator capacity, present in the human airways. There is recent evidence that PACAP decreases the release of proinflammatory cytokines. We have previously shown that PACAP inhibits neutrophil chemotaxis, but altogether little is known about the effects of PACAP on granulocytes. The present study was designed to investigate if PACAP and the closely related peptide vasoactive intestinal peptide (VIP) could affect the cell surface expression of CD11b, CD63 and CD66b in human neutrophils. Neutrophils isolated from 12 healthy blood donors were incubated with either PACAP or VIP, and the expression of neutrophil cell surface markers was assessed using flowcytometry. Neutrophils incubated with PACAP38 exhibited a marked, concentration-dependent increase in their expression of CD11b, CD63 and CD66b. In contrast, neutrophils incubated with VIP showed no increase of the investigated surface markers. This indicates a role for PACAP in granulocyte activation, mediated via a pathway not shared with VIP. Together with the previously presented data on leukocyte migration it suggests that PACAP acts as a regulator of neutrophil inflammation.
authors.affiliation: Laboratory of Clinical and Experimental Allergy Research, Department of Otorhinolaryngology, Malmö University Hospital, Malmö, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Kinhult
authors.forename: Arne
authors.initials: A
authors.lastname: Egesten
authors.forename: Rolf
authors.initials: R
authors.lastname: Uddman
authors.forename: Lars Olaf
authors.initials: LO
authors.lastname: Cardell
mesh_terms: D015703:Antigens, CD / Q000187:drug effects / Q000378:metabolism*; D000954:Antigens, Surface; D039481:CD11b Antigen / Q000187:drug effects / Q000378:metabolism*; D015815:Cell Adhesion Molecules; D004306:Dose-Response Relationship, Immunologic; D058851:GPI-Linked Proteins; D006801:Humans; D009479:Neuropeptides / Q000494:pharmacology*; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
chemical_list: C495401:ADCYAP1 protein, human; D015703:Antigens, CD; D000954:Antigens, Surface; D039481:CD11b Antigen; C504120:CEACAM8 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D009479:Neuropeptides; D010446:Peptide Fragments; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; C474275:antigen CD63b, human; C072440:vasoactive intestinal peptide (1-11); D014660:Vasoactive Intestinal Peptide