title: TPA stimulation culture for improved detection of t(11;14)(q13;q32) in mantle cell lymphoma.
abstract: Cytogenetic analysis of mantle cell lymphoma (MCL), characterized by the presence of t(11;14)(q13;q32) translocation, is often difficult because of the low proliferating rate of MCL cells and the presence of normal cells in bone marrow which may interfere with growth of MCL cells. We describe herein a TPA (12-O-tetradecanoylphorbol 13-acetate) stimulated culture to improve detection of t(11;14)(q13;q32) in 20 MCL patients regardless of the samples used.
authors.affiliation: Laboratoire de Génétique Médicale, Barre Nord Hôpital Jeanne de Flandre, CHRU de Lille, Lille, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Barouk-Simonet
authors.forename: Joris
authors.initials: J
authors.lastname: Andrieux
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Copin
authors.forename: Nathalie
authors.initials: N
authors.lastname: Grardel-Duflos
authors.forename: Pauline
authors.initials: P
authors.lastname: Huyghe
authors.forename: Jean-Hugues
authors.initials: JH
authors.lastname: Patte
authors.forename: Claude
authors.initials: C
authors.lastname: Preudhomme
authors.forename: Bruno
authors.initials: B
authors.lastname: Quesnel
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Laï
mesh_terms: D000328:Adult; D000368:Aged; D018929:Cell Culture Techniques / Q000379:methods; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D005260:Female; D006801:Humans; D020522:Lymphoma, Mantle-Cell / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D013755:Tetradecanoylphorbol Acetate