The level of 8-hydroxyguanine, a possible repair product of oxidative DNA damage, is higher in urine of cancer patients than in control subjects.
Using high-performance liquid chromatography prepurification/isotope dilution gas chromatography/mass spectrometry technique, we examined whether the amount of 8-hydroxyguanine and 8-hydroxy-2'-deoxyguanosine excreted into urine is higher in cancer patients with advanced-stage disease than in the control group. The control group consisted of 38 healthy subjects, and the patient group comprised 42 cancer patients suffering from metastasis of their primary tumors into the bones. We have found that the amount of the modified base (but not the nucleoside) excreted into urine is about 50% higher in cancer patients than in the control group. Because the presence of the modified base in urine may represent the primary repair product of oxidative DNA damage in vivo, our results suggest an important role of DNA glycosylases (most likely OGG1) in removal of the damage induced as a result of cancer development.
Rozalski
Rafal
R
Department of Clinical Biochemistry, The Ludwik Rydygier Medical University in Bydgoszcz, Karlowicza 24, 85-092 Bydgoszcz, Poland.
Gackowski
Daniel
D
Roszkowski
Krzysztof
K
Foksinski
Marek
M
Olinski
Ryszard
R
D014408:Biomarkers, Tumor / Q000032:analysis*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage; D045647:DNA Glycosylases; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine*; D006801:Humans; D008875:Middle Aged; D009699:N-Glycosyl Hydrolases / Q000494:pharmacology; D018384:Oxidative Stress; D014594:Uterine Neoplasms / Q000473:pathology*
D014408:Biomarkers, Tumor; C024829:8-hydroxyguanine; D006147:Guanine; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases