Heat acclimation-induced elevated glycogen, glycolysis, and low thyroxine improve heart ischemic tolerance.
Based on our observations of energy sparing in heat-acclimated (AC) rat hearts, we investigated whether changes in preischemic glycogen level, glycolytic rate, and plasma thyroxine level mediate cardioprotection induced in these hearts during ischemia-reperfusion insults. Control (C) (24 degrees C), AC (34 degrees C, 30 days), acclimated-euthyroid (34 degrees C + 3 ng/ml l-thyroxine), and control hypothyroid (24 degrees C + 0.02% 6-n-propyl-2-thiouracil) groups were studied. Preischemic glycogen was higher in AC than in C hearts [39.0 +/- 8.5 vs. 19.2 +/- 4.2 (SE) micromol glucose/g wet wt; P < 0.0006], and the lactate produced vs. glycogen level during total ischemia ((13)C-NMR spectroscopy) was markedly slower (AC: -0.82x, r = 0.98 vs. C: -4.7x, r = 0.9). Time to onset of ischemic contracture was lengthened, and the fraction of hearts experiencing ischemic contracture was lowered. Pulse pressure recovery was improved in AC compared with C animals before, but not after, absolute sodium iodoacetate-induced glycolysis inhibition. Acclimated-euthyroid hearts exhibited decreased ischemic tolerance, whereas induced hypothyroidism in C improved cardiotolerance. Thus higher preischemic glycogen and slowed glycolysis are associated with hypothyroidism and are likely important mediators of the improved ischemic tolerance exhibited by AC hearts.
Eynan
Mirit
M
Division of Physiology, Hadassah Schools of Dental Medicine and Medicine, The Hebrew University, Jerusalem 91120, Israel.
Knubuvetz
Tanya
T
Meiri
Uri
U
Navon
Gil
G
Gerstenblith
Gary
G
Bromberg
Zohar
Z
Hasin
Yonathan
Y
Horowitz
Michal
M
D000064:Acclimatization / Q000502:physiology*; D000818:Animals; D002247:Carbon Isotopes; D005947:Glucose / Q000493:pharmacokinetics; D006003:Glycogen / Q000378:metabolism*; D006019:Glycolysis / Q000502:physiology*; D006358:Hot Temperature; D007037:Hypothyroidism / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010807:Physical Endurance / Q000502:physiology; D051381:Rats; D013974:Thyroxine / Q000097:blood*
D002247:Carbon Isotopes; D019344:Lactic Acid; D006003:Glycogen; D005947:Glucose; D013974:Thyroxine