[Effects of Helicobacter pylori infection on porcine gastric epithelial cell proliferation].
OBJECTIVE
To investigate the association between porcine gastric epithelial cell proliferation and Helicobacter pylori (Hp) infection.


METHODS
Animal models of gastritis associated with Hp infection were established using "Chinese No.1 pigs". The expressions of proliferating cell nuclear antigen (PCNA) and DNA polyploid content in porcine gastric epithelial cells were quantitatively assayed by means of immunohistochemistry and Feulgen stain respectively.


RESULTS
The values of PCNA labeling index (LI) in the porcine gastric epithelial cells of the experimental group were significantly higher than those of the control group (40.95+/-3.60 vs 29.4+/-12.82, P<0.01). The DNA diploid or approximate diploid content in the gastric mucosa of the pigs was significantly lower (70.78% vs 90.65%, P<0.01), but the proliferation polyploid and non-doubleploid of DNA significantly higher in the experimental group than in the control group (P<0.01).


CONCLUSIONS
Hp infection may play a role to enhance the proliferation of gastric epithelial cells in pigs.
Song
Hong
H
Department of Gastroenterology, General Hospital of Guangzhou Command, Guangzhou 510010, China.
Sun
Gui-Hua
GH
Huang
Xiao-Rang
XR
Lai
Ri-Quan
RQ
Luo
Zhu-Quan
ZQ
Zhang
Wei
W
Tian
Ye
Y
Deng
Yong-Jian
YJ
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D005753:Gastric Mucosa / Q000473:pathology*; D016481:Helicobacter Infections / Q000473:pathology*; D016480:Helicobacter pylori / Q000502:physiology*; D013552:Swine