Application of PCR and probe hybridization techniques in detection of airborne fungal spores in environmental samples.
Specific PCR amplification and probe hybridization techniques were applied to examine the compositions of airborne fungi in samples from three different environments. The results from microscopic and CFU counting were compared to those of the molecular-based detections. The detection sensitivity for PCR amplifications was 9 to 73 spores and 1.3 to 19.3 CFUs per PCR reaction. The hybridization detection limit was 2 to 4 spores and 0.2 to 1.2 CFU. The hybridization method was more sensitive than PCR amplification and showed less variation among samples. Using specific PCR primers and probes we identified the presence of several fungal groups and species in the air samples. Specific detections through probe hybridization to PCR products amplified with universal or group-specific fungal primers have promising applications in the examination of air samples for environmental monitoring.
Wu
Zhihong
Z
Programme for Chemical Exposure Assessment, National Institute for Working Life, Umeå, Sweden.
Blomquist
Göran
G
Westermark
Sven-Olof
SO
Wang
Xiao-Ru
XR
D016902:Air Pollution, Indoor / Q000032:analysis*; D017931:DNA Primers; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D005658:Fungi / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013170:Spores
D017931:DNA Primers; D015342:DNA Probes; D004271:DNA, Fungal