Construction of a 1.2-Mb BAC/PAC contig of the porcine gene RYR1 region on SSC 6q1.2 and comparative analysis with HSA 19q13.13.
We screened a porcine bacterial artificial chromosome (BAC) and a P1 derived artificial chromosome (PAC) library to construct a sequence-ready approximately 1.2-Mb BAC/PAC contig of the ryanodine receptor-1 gene (RYR1) region on porcine chromosome (SSC) 6q1.2. This genomic segment is of special interest because it harbors the locus for stress susceptibility in pigs and a putative quantitative trait locus for muscle growth. Detailed physical mapping of this gene-rich region allowed us to assign to this contig 17 porcine genes orthologous to known human chromosome 19 genes. Apart from the relatively well-characterized porcine gene RYR1, the other 16 genes represent novel chromosomal assignments and 14 genes have been cloned for the first time in pig. Comparative analysis of the porcine BAC/PAC contig with the human chromosome (HSA) 19q13.13 map revealed a completely conserved gene order of this segment between pig and human. A detailed porcine-human-mouse comparative map of this region was constructed.
Martins-Wess
Flávia
F
Institute of Animal Breeding and Genetics, School of Veterinary Medicine Hannover, Bünteweg 17p, Hannover, Germany.
Voss-Nemitz
Rodja
R
Drögemüller
Cord
C
Brenig
Bertram
B
Leeb
Tosso
T
D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D027042:Chromosomes, Artificial, P1 Bacteriophage; D002888:Chromosomes, Human, Pair 19; D006801:Humans; D020161:Physical Chromosome Mapping; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics*; D013552:Swine; D026801:Synteny
D019837:Ryanodine Receptor Calcium Release Channel