[Effect of Helicobacter pylori infection on gastric mucosal cell proliferation in Mongolian gerbils].
OBJECTIVE
To explore the mechanism that Helicobacter pylori (H. pylori) infection can induce adenocarcinoma in Mongolian gerbil model with long-term H. pylori infection.


METHODS
Mongolian gerbil model with long-term H. pylori infection was established by inoculation H. pylori NCTC 11637 strain, and immunohistochemical straining and in situ hybridization were employed to observe changes in gastric mucosal cell proliferation due to H. pylori infection.


RESULTS
Mongolian gerbil model with long-term H. pylori infection was successfully established. Immunohistochemical staining of 5'-bromodeoxyuridine (BrdU) and proliferating cell nuclei antigen (PCNA) showed that H. pylori infection induced the increase in gastric mucosal cell proliferation (P<0.05), and in situ hybridization of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) revealed elevated expressions of EGF mRNA and EGFR mRNA with time passage after H. pylori infection (P<0.05).


CONCLUSIONS
H. pylori inoculation can induce abnormality in gastric mucosal cell proliferation, which is instrumental for the progression from chronic gastritis to glandular atrophy, intestinal metaplasis and ultimately to atypical hyperplasia. The abnormal expressions of EGF and EGFR may be the key element for abnormality of gastric mucosal cell proliferation.
Yao
Yong-Li
YL
Institute of Digestive Diseases of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Xu
Bo
B
Song
Yu-Gang
YG
Zhang
Wan-Dai
WD
Zhang
Ya-Li
YL
Zhang
Zhen-Shu
ZS
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D004195:Disease Models, Animal; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000382:microbiology / Q000473:pathology*; D016480:Helicobacter pylori; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000473:pathology; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism
D018809:Proliferating Cell Nuclear Antigen; D001973:Bromodeoxyuridine