title: Oat bran stimulates bile acid synthesis within 8 h as measured by 7alpha-hydroxy-4-cholesten-3-one.
abstract: BACKGROUND
  Oat bran contains soluble fibers, such as beta-glucan, that increase bile acid excretion and thus decrease serum cholesterol. Bile acid synthesis correlates with serum concentrations of the metabolite 7alpha-hydroxy-4-cholesten-3-one (alpha-HC).
  
  
  OBJECTIVE
  The objective was to investigate whether consumption of beta-glucan from oat bran increases bile acid synthesis, as measured by the serum alpha-HC concentration, within hours after consumption in response to the loss of bile acids from the liver.
  
  
  METHODS
  In a randomized, single-blind, wheat bran-controlled study with crossover design, 8 subjects were served a controlled diet during 2 periods of 3 d each, with an 11-d washout between the periods. Breakfast included either 75 g extruded oat bran, of which 11 g was beta-glucan, or 75 g wheat bran, of which 1 g was beta-glucan. Alpha-HC was measured by HPLC on each day at 0, 12, and 24 h after breakfast and also at 8 h after breakfast on the first day.
  
  
  RESULTS
  After 8 and 12 h of the oat bran diet period, the serum alpha-HC concentration was 84% (P = 0.012) and 92% (P = 0.017) higher, respectively, than that before breakfast. Serum concentrations returned to the baseline value after 24 h. Wheat bran did not influence serum alpha-HC concentrations.
  
  
  CONCLUSIONS
  Consumption of beta-glucan from oat bran nearly doubled the serum alpha-HC concentration within 8 h, indicating increased bile acid synthesis. alpha-HC in serum could be used as a marker of increased bile acid excretion induced by the diet.
authors.affiliation: Department of Clinical Nutrition, Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Andersson
authors.forename: Lars
authors.initials: L
authors.lastname: Ellegård
authors.forename: Henrik
authors.initials: H
authors.lastname: Andersson
mesh_terms: D000328:Adult; D018554:Avena / Q000737:chemistry; D001647:Bile Acids and Salts / Q000096:biosynthesis*; D002783:Cholestenones / Q000097:blood*; D018592:Cross-Over Studies; D004032:Diet; D004043:Dietary Fiber / Q000378:metabolism; D005936:Glucans / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D009994:Osmolar Concentration; D020033:Protein Isoforms / Q000378:metabolism; D016037:Single-Blind Method; D013997:Time Factors
chemical_list: D001647:Bile Acids and Salts; D002783:Cholestenones; D004043:Dietary Fiber; D005936:Glucans; D020033:Protein Isoforms; C002656:7 alpha-hydroxy-4-cholesten-3-one