title: Inhibition of phosphatidylcholine synthesis induces expression of the endoplasmic reticulum stress and apoptosis-related protein CCAAT/enhancer-binding protein-homologous protein (CHOP/GADD153).
abstract: Inhibition of de novo synthesis of phosphatidylcholine (PC) by some anti-cancer drugs such as hexadecylphosphocholine leads to apoptosis in various cell lines. Likewise, in MT58, a mutant Chinese hamster ovary (CHO) cell line containing a thermo-sensitive mutation in CTP:phosphocholine cytidylyltransferase (CT), an important regulatory enzyme in the CDP-choline pathway, inhibition of PC synthesis causes PC depletion. Cellular perturbations like metabolic insults and unfolded proteins can be registered by the endoplasmic reticulum (ER) and result in ER stress responses, which can lead eventually to apoptosis. In this study we investigated the effect of PC depletion on the ER stress response and ER-related proteins. Shifting MT58 cells to the non-permissive temperature of 40 degrees C resulted in PC depletion via an inhibition of CT within 24 h. Early apoptotic features appeared in several cells around 30 h, and most cells were apoptotic within 48 h. The temperature shift in MT58 led to an increase of pro-apoptotic CCAAT/enhancer-binding protein-homologous protein (CHOP; also known as GADD153) after 16 h, to a maximum at 24 h. Incubation of wild-type CHO-K1 or CT-expressing MT58 cells at 40 degrees C did not induce differences in CHOP protein levels in time. In contrast, expression of the ER chaperone BiP/GRP78, induced by an increase in misfolded/unfolded proteins, and caspase 12, a protease specifically involved in apoptosis that results from stress in the ER, did not differ between MT58 and CHO-K1 cells in time when cultured at 40 degrees C. Furthermore, heat-shock protein 70, a protein that is stimulated by accumulation of abnormal proteins and heat stress, displayed similar expression patterns in MT58 and K1 cells. These results suggest that PC depletion in MT58 induces the ER-stress-related protein CHOP, without raising a general ER stress response.
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine and Institute of Biomembranes, University of Utrecht, The Netherlands. MHM.vanderSanden@vet.uu.nl
authors.forename: Michiel H M
authors.initials: MH
authors.lastname: van der Sanden
authors.forename: Martin
authors.initials: M
authors.lastname: Houweling
authors.forename: Lambert M G
authors.initials: LM
authors.lastname: van Golde
authors.forename: Arie B
authors.initials: AB
authors.lastname: Vaandrager
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism*; D016466:CHO Cells; D002352:Carrier Proteins / Q000378:metabolism; D053454:Caspase 12; D020169:Caspases / Q000378:metabolism; D019916:Choline-Phosphate Cytidylyltransferase / Q000235:genetics / Q000378:metabolism; D006224:Cricetinae; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones / Q000378:metabolism; D009154:Mutation; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D013312:Stress, Physiological; D013696:Temperature; D051742:Transcription Factor CHOP; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D002352:Carrier Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D010713:Phosphatidylcholines; D014157:Transcription Factors; D051742:Transcription Factor CHOP; D019916:Choline-Phosphate Cytidylyltransferase; D053454:Caspase 12; D020169:Caspases