The complete genome sequence of gill-associated virus of Penaeus monodon prawns indicates a gene organisation unique among nidoviruses.
We report here a 5596 nt sequence comprising the 3'-end of the (+) ssRNA genome of gill-associated virus (GAV), an invertebrate nidovirus of Penaeus monodon prawns. The sequence extends from a subgenomic RNA start site 35 nt upstream of the 4923 nt ORF3 gene to a 3'-poly(A) tail of the 26235 nt genome of GAV. The putative 1640 amino acid (aa) ORF3 protein (MW = 182049 Da, pI = 6.62) contains 15 potential N-linked glycosylation sites, 15 potential O-linked glycosylation sites and six highly hydrophobic regions predicted to represent transmembrane (TM) domains. Three of the predicted TM domains occur in the amino-terminal 228 aa, two in the central portion, and one near the carboxy-terminus of ORF3. Only one short (83 aa) open reading frame (ORF4) was identified between ORF3 and the 3'-poly(A) tail. Completion of the genome sequence of GAV has revealed a gene organisation unique among nidoviruses in there is no discrete membrane protein gene and that the putative ORF3 spike glycoprotein gene resides downstream of a gene (ORF2) encoding a structural protein associated with nucleocapsids.
Cowley
J A
JA
Cooperative Research Centre for Aquaculture, CSIRO Livestock Industries, Long Pocket Laboratories, Indooroopilly, Australia. jeff.cowley@csiro.au
Walker
P J
PJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D033364:Decapoda / Q000821:virology*; D016679:Genome, Viral; D005880:Gills / Q000821:virology*; D008969:Molecular Sequence Data; D028381:Nidovirales / Q000145:classification / Q000235:genetics*; D016366:Open Reading Frames; D012367:RNA, Viral / Q000737:chemistry*
D012367:RNA, Viral