Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine values measured by an ELISA correlated well with measurements by high-performance liquid chromatography with electrochemical detection.
Measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) has recently become more popular as a means of assessing oxidative stress in the human body. Although using high-performance liquid chromatography with electrochemical detection (HPLC-ECD) is a reliable method of measuring 8-oxo-dG, easier and simpler alternative methods may be preferred, if they are quantitatively accurate. The ELISA method is the likeliest candidate for a useful alternative. Anti-8-oxo-dG monoclonal antibody N45.1 has been shown to have better specificity for 8-oxo-dG than other anti-8-oxo-dG antibodies, but the urinary 8-oxo-dG values measured by ELISA using N45.1 have been claimed to only weakly correlate with the values obtained by HPLC-ECD. Because the commercial ELISA kit has been improved, we compared the urinary 8-oxo-dG values measured by the ELISA with the values obtained by HPLC-ECD. We sampled the urine of 72 healthy Japanese individuals and measured their urinary 8-oxo-dG levels by the ELISA with appropriate controls and by HPLC-ECD. When X was defined as the values of 8-oxo-dG measured by HPLC-ECD, and Y was defined as the values of 8-oxo-dG measured by the ELISA, simple regression analysis showed the most likely relationship to be Y = 1.83X + 0.8. The correlation coefficient was 0.88, which indicated a good correlation between X and Y. These results show that the ELISA can be applied to studies comparing relative urinary 8-oxo-dG values among several groups, if the studies do not require determination of the exact concentration of 8-oxo-dG in urine.
Yoshida
Rie
R
National Institute of Industrial Health, Kawasaki 214-8585, Japan.
Ogawa
Yasutaka
Y
Kasai
Hiroshi
H
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine