Quantification of cytochrome P450 aromatase transcripts before and during luteal phase in rabbit.
The aim of the present study was to quantify the promoter II- and I.r-derived transcripts of p450 aromatase gene during follicular stages and during corpus luteum formation in the rabbit. An ovulatory dose of hCG induced, first the disappearance of 90% of aromatase transcripts since 6 h before ovulation, and second a gradual decrease during pseudopregnancy. Individual quantification of both the promoter-derived transcripts showed that promoter II-derived transcript was the main transcript expressed both during follicular phase and pseudopregnancy, but kinetics of disappearance were not similar between both the promoter-derived transcripts. Moreover, hCG up-regulates aromatase expression in vitro in luteal tissue but estradiol, which was without effect on aromatase expression in preovulatory granulosa cells, down-regulates this expression in luteal tissue. In conclusion, the regulation of P450 aromatase in rabbit is mainly under control of promoter II regardless of which cyclic stage is studied. Moreover, we reported an opposite effect of estradiol on aromatase expression in vitro between follicular and luteal cells.
Bouraïma
H
H
Laboratoire de Biochimie, UPRES EA 2608-USC INRA, Esplanade de la paix, Université de Caen, 14032, Caen, France. helene.bouraima@wanadoo.fr
Hanoux
V
V
Mittre
H
H
Féral
C
C
Benhaïm
A
A
Leymarie
P
P
D000818:Animals; D001141:Aromatase / Q000235:genetics*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005498:Follicular Phase; D005786:Gene Expression Regulation / Q000187:drug effects; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D008184:Luteal Cells / Q000187:drug effects / Q000378:metabolism; D008183:Luteal Phase / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011555:Pseudopregnancy / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis* / Q000187:drug effects; D011817:Rabbits
D006063:Chorionic Gonadotropin; D012333:RNA, Messenger; D004958:Estradiol; D001141:Aromatase