title: Development of a RT-nested PCR test detecting pigeon Paramyxovirus-1 directly from organs of infected animals.
abstract: A RT-nested PCR that amplifies part of the conserved nucleoprotein gene of avian Paramyxovirus type 1 is described. The technique allowed the detection of pigeon Paramyxovirus type 1 (pPMV-1) virus directly from a wide range of infected chicken and pigeon organs, and should be able to detect typical Newcastle disease viruses too. Compared with the reference method, the developed RT-nested PCR was found more sensitive, as it was able to detect virus genome in infected pigeon organs at late stage of infection, when virus isolation failed. Such a molecular technique represents an alternative method of diagnosis for research purposes on pPMV-1 variants, for example to study pathogenesis aspects of the infection or to assess the efficacy of vaccines.
authors.affiliation: AFSSA-site de Ploufragan, U VIPAC, BP53, 22440 Ploufragan, France.
authors.forename: C
authors.initials: C
authors.lastname: Barbezange
authors.forename: V
authors.initials: V
authors.lastname: Jestin
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000821:virology; D002645:Chickens; D010856:Columbidae; D017931:DNA Primers; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000302:isolation & purification*; D009928:Organ Specificity; D011201:Poultry Diseases / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers