title: MHC class II genes in European wolves: a comparison with dogs.
abstract: The genome of the grey wolf, one of the most widely distributed land mammal species, has been subjected to both stochastic factors, including biogeographical subdivision and population fragmentation, and strong selection during the domestication of the dog. To explore the effects of drift and selection on the partitioning of MHC variation in the diversification of species, we present nine DQA, 10 DQB, and 17 DRB1 sequences of the second exon for European wolves and compare them with sequences of North American wolves and dogs. The relatively large number of class II alleles present in both European and North American wolves attests to their large historical population sizes, yet there are few alleles shared between these regions at DQB and DRB1. Similarly, the dog has an extensive array of class II MHC alleles, a consequence of a genetically diverse origin, but allelic overlap with wolves only at DQA. Although we might expect a progression from shared alleles to shared allelic lineages during differentiation, the partitioning of diversity between wolves and dogs at DQB and DRB1 differs from that at DQA. Furthermore, an extensive region of nucleotide sequence shared between DRB1 and DQB alleles and a shared motif suggests intergenic recombination may have contributed to MHC diversity in the Canidae.
authors.affiliation: Department of Evolutionary Biology, EBC, Uppsala University, Norbyvagen 18D, 752 36 Uppsala, Sweden. jennifer.seddon@ebc.uu.se
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Seddon
authors.forename: Hans
authors.initials: H
authors.lastname: Ellegren
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D004247:DNA; D004285:Dogs / Q000235:genetics*; D019143:Evolution, Molecular; D005802:Genes, MHC Class II; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D019596:Wolves / Q000235:genetics*
chemical_list: D004247:DNA