title: Klebsiella pneumoniae KE-1 degrades endosulfan without formation of the toxic metabolite, endosulfan sulfate.
abstract: For bioremediation of toxic endosulfan, endosulfan degradation bacteria, which do not form toxic endosulfan sulfate, were isolated from various soil samples using endosulfan as sole carbon and energy source. Among the 40 isolated bacteria, strain KE-1, which was identified as Klebsiella pneumoniae by physiological and 16S rDNA sequence analysis, showed superior endosulfan degradation activity. Analysis of culture pH, growth, free sulfate and endosulfan and its metabolites demonstrated that KE-1 biologically degrades 8.72 microg endosulfan ml(-1) day(-1) when incubated with 93.9 microg ml(-1) endosulfan for 10 days without formation of toxic endosulfan sulfate. Our results suggest that K. pneumoniae KE-1 degraded endosulfan by a non-oxidative pathway and that strain KE-1 has potential as a biocatalyst for endosulfan bioremediation.
authors.affiliation: The School of Bioresource Science, Andong National University, South Korea. gskwon@andong.ac.kr
authors.forename: Gi-Seok
authors.initials: GS
authors.lastname: Kwon
authors.forename: Jang-Eok
authors.initials: JE
authors.lastname: Kim
authors.forename: Taek-Kyum
authors.initials: TK
authors.lastname: Kim
authors.forename: Ho-Yong
authors.initials: HY
authors.lastname: Sohn
authors.forename: Sung-Cheol
authors.initials: SC
authors.lastname: Koh
authors.forename: Kee-Sun
authors.initials: KS
authors.lastname: Shin
authors.forename: Dong-Geol
authors.initials: DG
authors.lastname: Kim
mesh_terms: D001673:Biodegradation, Environmental; D004275:DNA, Ribosomal / Q000032:analysis; D004726:Endosulfan / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism
chemical_list: D004275:DNA, Ribosomal; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D013431:Sulfates; D004726:Endosulfan