title: Kinetic proofreading in receptor-mediated transduction of cellular signals: receptor aggregation, partially activated receptors, and cytosolic messengers.
abstract: Signaling by the T cell receptor (TCR), and the related immunoreceptor Fc epsilon RI, is sensitive to ligand-receptor binding kinetics. Differences in the rate at which a ligand dissociates from a receptor cause disproportionate differences in signaling events and cellular responses to ligand-receptor engagement. Analysis of a simple mathematical model, developed by McKeithan (1995, Proc. Natl. Acad. Sci. USA, 92, 5042-5046), has indicated that such sensitivity to binding kinetics is expected if a bound receptor must complete a cascade of modifications before generating a productive signal. However, recent experiments show that some cellular responses mediated by immunoreceptors escape from the control of kinetic proofreading, in the sense that these responses do not exhibit the expected sensitivity to the lifetime of a ligand-receptor bond. Here, we use an extended form of the McKeithan model to investigate possible explanations for such exceptions to the kinetic proofreading rule. We examine cellular responses triggered by cytosolic messengers, which are activated by modified receptors, and responses triggered by receptors in intermediate states of modification, i.e., receptors that have not progressed through the full series of potential modifications. Receptor aggregation is also considered. We find that the expected relationship between ligand-receptor binding kinetics and cellular responses can change significantly when signal transduction depends on a messenger or a partially modified receptor. In particular, cellular responses triggered by a messenger, such as a transcription factor that translocates from the membrane to the nucleus after receptor-mediated activation, can be sensitive or insensitive to a change in the lifetime of a ligand-receptor bond, depending on the parameters that govern the activation and decay of a messenger.
authors.affiliation: Theoretical Biology & Biophysics, Theoretical Division, Los Alamos National Laboratory, Mail Stop K710, T-10, Los Alamos, NM 87545, USA. wish@lanl.gov
authors.forename: William S
authors.initials: WS
authors.lastname: Hlavacek
authors.forename: Antonio
authors.initials: A
authors.lastname: Redondo
authors.forename: Carla
authors.initials: C
authors.lastname: Wofsy
authors.forename: Byron
authors.initials: B
authors.lastname: Goldstein
mesh_terms: D000818:Animals; D003600:Cytosol / Q000502:physiology; D007700:Kinetics; D008024:Ligands; D008954:Models, Biological; D051381:Rats; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D017455:Receptors, IgE / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D008024:Ligands; D011948:Receptors, Antigen, T-Cell; D017455:Receptors, IgE