title: Expression of COUP-TFII in metabolic tissues during development.
abstract: In mammals, the COUP-TF-family consisting of two structurally related proteins, COUP-TFI and COUP-TFII belongs to the orphan member of the steroid/thyroid hormone receptor superfamily. In an attempt to gain insights into the role of COUP-TFII, we examined developmental expression pattern of the mouse COUP-TFII focusing our studies on endoderm-derived tissues, pancreas and liver in particular. Independent lines of transgenic mice expressing Escherichia coli beta-galactosidase driven by the COUP-TFII promoter were generated. Embryonic expression of the beta-gal protein at day 9 of gestation was detected in the notochord, the ventral neural tube and, interestingly, in the gut endoderm, a site where COUP-TFII has not been detected previously. Between 9.5 and 11.5 dpc, beta-gal expression pattern that was established earlier persisted and sections revealed a staining of the common atrial chamber of the heart. At 15.5 dpc, beta-gal activity was found in all endoderm-derived tissues. We found that COUP-TFII mRNA and protein were present in fetal and adult hepatocytes. Finally, COUP-TFII expression was detected in pancreas, as judged by co-expression of the beta-gal in some of the glucagon and PDX1 positive-cells at 12.5 dpc and co-expression with insulin positive-cells at 15.5 dpc. In adult pancreas, COUP-TFII protein was present in the endocrine islet cells.
authors.affiliation: Institut Cochin, IFR 116, INSERM U567, Département de Génétique, Développement et Physiopathologies Moléculaires, 24 rue du Faubourg Saint Jacques, 75014 Paris, France.
authors.forename: Pili
authors.initials: P
authors.lastname: Zhang
authors.forename: Myriam
authors.initials: M
authors.lastname: Bennoun
authors.forename: Cécile
authors.initials: C
authors.lastname: Gogard
authors.forename: Pascale
authors.initials: P
authors.lastname: Bossard
authors.forename: Isabelle
authors.initials: I
authors.lastname: Leclerc
authors.forename: Axel
authors.initials: A
authors.lastname: Kahn
authors.forename: Mireille
authors.initials: M
authors.lastname: Vasseur-Cognet
mesh_terms: D000818:Animals; D051837:COUP Transcription Factor II; D051836:COUP Transcription Factors; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D004707:Endoderm / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D006321:Heart / Q000196:embryology; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D018398:Homeodomain Proteins; D007150:Immunohistochemistry; D008099:Liver / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009206:Myocardium / Q000378:metabolism; D010179:Pancreas / Q000196:embryology / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011987:Receptors, Steroid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D051837:COUP Transcription Factor II; D051836:COUP Transcription Factors; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C497587:Nr2f2 protein, mouse; D012333:RNA, Messenger; D011987:Receptors, Steroid; D015534:Trans-Activators; D014157:Transcription Factors; C084010:pancreatic and duodenal homeobox 1 protein; D005934:Glucagon; D001616:beta-Galactosidase