title: E26 leukemia virus converts primitive erythroid cells into cycling multilineage progenitors.
abstract: Acute chicken leukemia retroviruses, because of their capacity to readily transform hematopoietic cells in vitro, are ideal models to study the mechanisms governing the cell-type specificity of oncoproteins. Here we analyzed the transformation specificity of 2 acute chicken leukemia retroviruses, the Myb-Ets- encoding E26 virus and the ErbA/ErbB-encoding avian erythroblastosis virus (AEV). While cells transformed by E26 are multipotent (designated "MEP" cells), those transformed by AEV resemble erythroblasts. Using antibodies to separate subpopulations of precirculation yolk sac cells, both viruses were found to induce the proliferation of primitive erythroid progenitors within 2 days of infection. However, while AEV induced a block in differentiation of the cells, E26 induced a gradual shift in their phenotype and the acquisition of the potential for multilineage differentiation. These results suggest that the Myb-Ets oncoprotein of the E26 leukemia virus converts primitive erythroid cells into proliferating definitive-type multipotent hematopoietic progenitors.
authors.affiliation: Biomedical Research Centre, Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
authors.forename: Kelly M
authors.initials: KM
authors.lastname: McNagny
authors.forename: Thomas
authors.initials: T
authors.lastname: Graf
mesh_terms: D001355:Alpharetrovirus / Q000502:physiology*; D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000502:physiology; D001756:Blastoderm / Q000473:pathology / Q000821:virology; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D015672:Erythroid Precursor Cells / Q000473:pathology / Q000821:virology*; D006412:Hematopoietic Stem Cells / Q000473:pathology / Q000821:virology; D016130:Immunophenotyping; D039902:Multipotent Stem Cells / Q000473:pathology / Q000821:virology*; D014411:Neoplastic Stem Cells / Q000473:pathology / Q000821:virology*