The use of resonance energy transfer in high-throughput screening: BRET versus FRET.
Bioluminescence resonance energy transfer has developed in recent years as a new technique to study protein-protein interactions. Protein partners of interest are tagged with either luciferase or green fluorescent protein (GFP). Non-radiative energy transfer between the excited luciferase and the GFP permits the study of spatial relationships between the two partners. This technique constitutes an important tool for the study of the functional activity of different types of receptors, and can be used in sensitive, homogenous high-throughput screening assays.
Boute
Nicolas
N
Institut Cochin, CNRS UMR 8104, INSERM U567, Université Paris V, Département de Biologie Cellulaire, 22 Rue Méchain, 75014, Paris, France.
Jockers
Ralf
R
Issad
Tarik
T
D000818:Animals; D005453:Fluorescence; D049452:Green Fluorescent Proteins; D006801:Humans; D008156:Luciferases; D008163:Luminescent Measurements; D008164:Luminescent Proteins; D008967:Molecular Biology / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D008156:Luciferases