title: Adenoviral p53 gene therapy in head and neck squamous cell carcinoma cell lines.
abstract: We reconstructed the recombinant p53-expressing adenovirus and examined its infections and effects in head and neck squamous cell carcinoma cell lines. Eight human head and neck squamous cell carcinoma cell lines were infected by the recombinant adenovirus harboring the lacZ gene (AxCAiLacZ) or the wild-type p53 gene (AxCAip53), and the effects were investigated. The eight cell lines were successfully infected by AxCAiLacZ at a level of more than 50%. The survival of all 8 squamous cell lines were inhibited in the range from 8 to 26.7% by only one treatment of the AxCAip53 infection. This result suggested that p53 gene therapy might become a useful tool in head and neck squamous cell carcinoma treatment.
authors.affiliation: Department of Oral and Maxillofacial Radiology, Graduate School of Medicine and Dentistry, Okayama University, Okayama, Japan.
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Higuchi
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Asaumi
authors.forename: Jun
authors.initials: J
authors.lastname: Murakami
authors.forename: Toru
authors.initials: T
authors.lastname: Wakasa
authors.forename: Tetsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kuroda
authors.forename: Koichi
authors.initials: K
authors.lastname: Shibuya
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shigehara
authors.forename: Shoji
authors.initials: S
authors.lastname: Kawasaki
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Fukui
authors.forename: Kanji
authors.initials: K
authors.lastname: Kishi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hiraki
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D002470:Cell Survival; D017931:DNA Primers / Q000737:chemistry; D005697:Galactosides / Q000378:metabolism; D016158:Genes, p53; D015316:Genetic Therapy; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D006801:Humans; D007211:Indoles / Q000378:metabolism; D007763:Lac Operon; D016133:Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D017931:DNA Primers; D005697:Galactosides; D007211:Indoles; D001616:beta-Galactosidase; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside