title: WARTS tumor suppressor is phosphorylated by Cdc2/cyclin B at spindle poles during mitosis.
abstract: Identification of physiological substrates for Cdc2/cyclin B is crucial for understanding the functional link between mitotic events and Cdc2/cyclin B activation. A human homologue of the Drosophila warts tumor suppressor, termed WARTS, is a serine/threonine kinase and a dynamic component of the mitotic apparatus. We have found that Cdc2/cyclin B forms a complex with a fraction of WARTS in the centrosome and phosphorylates the Ser613 site of WARTS during mitosis. Immunocytochemical analysis has shown that the S613-phosphorylated WARTS appears in the spindle poles at prometaphase and disappears at telophase. Our findings suggest that Cdc/cyclin B regulates functions of WARTS on the mitotic apparatus.
authors.affiliation: Department of Tumor Genetics and Biology, Kumamoto University School of Medicine, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Morisaki
authors.forename: Toru
authors.initials: T
authors.lastname: Hirota
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Iida
authors.forename: Tomotoshi
authors.initials: T
authors.lastname: Marumoto
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Hara
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: Michio
authors.initials: M
authors.lastname: Kawasuzi
authors.forename: Takehisa
authors.initials: T
authors.lastname: Hiraoka
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Mimori
authors.forename: Norie
authors.initials: N
authors.lastname: Araki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Izawa
authors.forename: Masaki
authors.initials: M
authors.lastname: Inagaki
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Saya
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002850:Chromatography, Gel; D019926:Cyclin B / Q000378:metabolism*; D029721:Drosophila Proteins; D016147:Genes, Tumor Suppressor; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008938:Mitosis; D010766:Phosphorylation; D011233:Precipitin Tests; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000276:immunology / Q000378:metabolism*; D008941:Spindle Apparatus; D013347:Subcellular Fractions / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: C503698:CycB protein, Drosophila; D019926:Cyclin B; D029721:Drosophila Proteins; D011494:Protein Kinases; C092745:LATS1 protein, human; C093503:wts protein, Drosophila; D017346:Protein Serine-Threonine Kinases; D016203:CDC2 Protein Kinase