Physical mapping of 18S-25S rDNA and 5S rDNA in Lupinus via fluorescent in situ hybridization.
Double-target fluorescent in situ hybridization (FISH) was used to determine the genomic distribution of ribosomal RNA genes in five Lupinus species: L. cosentinii (2n=32), L. pilosus (2n=42), L. angustifolius (2n=40), L. luteus (2n=52) and L. mutabilis (2n=48). 18S-25S rDNA and 5S rDNA were used as probes. Some interspecific variation was observed in the number and size of the 18S-25S rDNA loci. All the studied species had one chromosome pair carrying 5S rDNA.
Naganowska
Barbara
B
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań Poland.
Zielińska
Anna
A
D018744:DNA, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D017343:Genes, Plant; D017404:In Situ Hybridization, Fluorescence; D031297:Lupinus / Q000235:genetics*; D020161:Physical Chromosome Mapping; D018749:RNA, Plant / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D013045:Species Specificity
D018744:DNA, Plant; D004275:DNA, Ribosomal; D018749:RNA, Plant; D012337:RNA, Ribosomal, 18S; D012338:RNA, Ribosomal, 23S; D012341:RNA, Ribosomal, 5S