Endocrine-mediated effects of UV-A irradiation on grass shrimp (Palaemonetes pugio) reproduction.
Although much is known regarding photoperiodic effects on crustacean egg production, the effects of ultraviolet (UV) light on reproduction has not been investigated. Likewise, little is known concerning the interaction between UV and xenobiotic exposure on crustacean reproductive cycles. In this study, male and female grass shrimp, Palaemonetes pugio, were exposed to sublethal concentrations of endosulfan (200 ng/l and 400 ng/l ES) under both white fluorescent (WF) and UV-A (315-400 nm) light conditions for 50 days in laboratory bioassays. Female endocrine (vitellogenin, ecdysteroids, and cholesterol), reproductive (percent gravid, clutch size), and embryo (days to hatch, hatching success, and hatching survival) responses were assessed. UV-exposure alone caused a significant (>4-fold) increase in total Palaemonetes pugio female egg production over the course of 50 days. Exposure to ES and UV significantly lowered the percentage of gravid females relative to UV controls, whereas ES-exposed shrimp under WF lighting did not exhibit these trends. Although higher vitellogenin concentrations and lower ecdysteroid titers were correlated with increased female egg production, cholesterol titers only exhibited a dose-dependent change when exposed to ES. Embryos from females exposed to UV had significantly lower ecdysteroid titers and shorter hatching times but there were no differences in embryo vitellogenin concentrations, hatching success, or hatching survival. These results indicate that UV-A exposure has a pronounced effect on grass shrimp (Palaemonetes pugio) reproduction and is likely mediated through 5-hydroxytrptamine (5-HT)-related neuroendocrine pathways. The implications for decapod aquaculture and evaluating chronic contaminant effects are discussed.
Volz
David C
DC
Department of Environmental Health Sciences, University of South Carolina, Columbia, SC 29208, USA.
Wirth
Edward F
EF
Fulton
Michael H
MH
Scott
Geoffrey I
GI
Block
David S
DS
Chandler
G Thomas
GT
D000818:Animals; D004305:Dose-Response Relationship, Drug; D026461:Ecdysteroids / Q000378:metabolism; D004527:Egg Proteins / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000528:radiation effects; D004726:Endosulfan / Q000633:toxicity*; D005260:Female; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides / Q000633:toxicity*; D008136:Longevity / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D033541:Palaemonidae / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D010777:Photochemistry; D012098:Reproduction / Q000187:drug effects / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014819:Vitellogenins / Q000378:metabolism
D026461:Ecdysteroids; D004527:Egg Proteins; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D014819:Vitellogenins; D004726:Endosulfan