Peptide biosensor for recognition of cross-species cell surface markers.
Biosensors based on phage display-derived peptides as biorecognition molecules were used for the detection of cell surface cross-species markers in tissue homogenates. The peptide selected for murine myofibers was immobilized onto the surface of an acoustic wave sensor by biotin-streptavidin coupling. To detect peptide-receptor interaction, the sensors were exposed to muscle and control (kidney, liver, brain) tissue homogenates. The sensor showed a strong response to murine muscle. The amplitudes of the responses to the feline muscle homogenates were lower compared to those of the murine muscle, while the same K(d) indicated that the peptide has cross-species affinity. In contrast, murine kidney, liver and brain homogenates produced insignificant responses. Specificity of the sensor was shown in a blocking experiment, as reduced signal was detected when muscle preparations were preincubated with free peptide. Additionally, when muscle-specific peptide was replaced with two different random control peptides, the sensors produced no response to murine muscle. Suitability of peptide ligands for a variety of species can be evaluated using this technology.
Samoylov
Alexandre M
AM
Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, AL 36849, USA.
Samoylova
Tatiana I
TI
Pathirana
Suram T
ST
Globa
Ludmila P
LP
Vodyanoy
Vitaly J
VJ
D000162:Acoustics / Q000295:instrumentation; D000818:Animals; D001665:Binding Sites; D015374:Biosensing Techniques; D001921:Brain / Q000378:metabolism; D002138:Calibration; D002415:Cats; D020650:Combinatorial Chemistry Techniques; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D013229:Stearic Acids / Q000378:metabolism
D019151:Peptide Library; D010455:Peptides; D013229:Stearic Acids; C031183:stearic acid