New human embryo liver cell lines obtained by stabilization and immortalization enhance in vitro clonal growth of cordonal blood cells.
We developed two new cell lines derived from embryo liver and tested their inductive capacity on in vitro clonal growth of cordonal blood (CB) hematopoietic cells. One line was stabilized and named BAEP2-WILD (W), and the other one was immortalized by retroviral transduction with SV40 Large T antigen and called BAEP2-SV40. Southern blot analysis demonstrated the integration of the Large T antigen gene in the BAEP2-SV40 cell genome, but this line did not display the expected growth arrest at the non-permissive temperature of 39 degrees C. Immunocytochemistry showed that BAEP2-SV40 cell line was positive for several cytokeratins and stromal markers (vimentin, desmin and laminin), as well as for epidermal growth factor (EGF), fibroblast growth factor (FGF) and their receptors (Rs). In contrast, BAEP2-W evidenced positivity only for cytokeratin-7 and laminin, and low positivity to EGF, EGF-R, FGF and FGF-R. BAEP2-SV40 cell line, but not BAEP2-W, expressed interleukin (IL)-1, IL-6, granulocyte macrophage-colony stimulating factor (GM-CSF), granulocyte-colony stimulating factor, stem cell factor and vascular-cell adhesion molecule-1 mRNAs, and secreted IL-6 and GM-CSF. Taken together, these findings could suggest that BAEP2-W cell line possesses the phenotype of fetal hepatocytes, while BAEP2-SV40 cell line has that of stromal cells. The supernatants conditioned by both cell lines stimulated the clonal growth of CB hematopoietic cells cultured on semisolid media deprived of growth factors and cytokines, the inductive capacity of the BAEP2-SV40 cell line being markedly higher than that of its wild counterpart, conceivably due to its ability to produce cytokines. Our study indicates that these two new cell lines, and especially BAEP2-SV40 one could be used in co-culture systems as feeder-layers for hematopoietic CB SC expansion in vitro.
Del Pup
Laura
L
Department of Pharmaceutical Sciences, School of Pharmacy, University of Padua, I-35121 Padua, Italy.
De Angeli
Sergio
S
Conconi
Maria Teresa
MT
Grandi
Claudio
C
Gamba
Pier Giorgio
PG
Parnigotto
Pier Paolo
PP
Nussdorfer
Gastone G
GG
D015415:Biomarkers; D002455:Cell Division; D002460:Cell Line; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D016207:Cytokines / Q000235:genetics; D004622:Embryo, Mammalian / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D007621:Karyotyping; D008099:Liver / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015415:Biomarkers; D016207:Cytokines