The positive effects of postnatal handling on defensive burying are more obvious in a situation that enlarges the potential coping responses.
A recent study reported few effects of postnatal handling in the defensive burying test. Since the importance of testing conditions has been emphasised in this paradigm, the lack of handling effects in this test could be attributed to the testing situation. Our experiment was carried out to test whether postnatal handling in DA/HAN strain rats have positive effects in two situations of the defensive burying test: a no-hide situation, in which avoidance of the probe was not possible, and a hide situation, in which animals were allowed to avoid the probe by sheltering in a hiding compartment. Our results showed no difference between control and handled rats defensive reactions in the no-hide situation. However, the general coping style of handled rats was different, mostly by a higher level of exploration of the apparatus and of the probe. In the hide situation, the time spent burying the probe was significantly lower in handled rats and they also spent less time in the hiding compartment. Those results, along with approach/avoidance behaviours directed towards the probe demonstrate that postnatal handling do have some positive effects in the defensive burying test. In addition, our results also point out that testing situations in which complex coping strategies are available are appropriate to test the effects of early stimulation such as handling.
Roy
Vincent
V
Laboratoire de Neurobiologie de l'Apprentissage, UFR des Sciences de Rouen, UPRES PSY.CO, EA 1780, 76821 Mont-Saint-Aignan, France. vincent.roy@univ-rouen.fr
Chapillon
Pierre
P
D000223:Adaptation, Psychological / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001007:Anxiety / Q000523:psychology*; D006185:Habituation, Psychophysiologic; D006234:Handling, Psychological; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains