Dynamic changes of matrix metalloproteinases in rat liver during the development of diethylnitrosamine-induced hepatocarcinoma.
OBJECTIVE
To study the dynamic changes of matrix metalloproteinases (MMPs) in the liver of rat during experimental hepatocarcinogenesis.


METHODS
Immunohistochemistry, gelatin zymography, and reverse transcriptase-PCR were used for detection of latent and active forms and mRNA of MMPs in each phases of carcinogenic stages.


RESULTS
MMPs expression was detected in normal and cirrhotic liver, which was most obvious in the cancer cells after the development of hepatic carcinoma. Normal tissue showed only low levels of MMPs expression, which kept increasing in the course of hepatocarcinogenesis, as was also the case with the corresponding mRNA.


CONCLUSIONS
Transcription and expression of MMPs keep increasing throughout the the process of hepatocarcinogenesis.
Gao
Yi
Y
Department of Organ Transplantation, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Zhang
Zhi
Z
Jiang
Ze-Sheng
ZS
Fang
Shi-Gang
SG
Sun
Er-Wei
EW
Wang
Yu
Y
Chen
Bao-Hua
BH
D000818:Animals; D002273:Carcinogens / Q000009:adverse effects; D004052:Diethylnitrosamine / Q000009:adverse effects; D007150:Immunohistochemistry / Q000379:methods; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D012333:RNA, Messenger; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002273:Carcinogens; D012333:RNA, Messenger; D004052:Diethylnitrosamine; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9