title: [Detection and analysis of Fas gene (exon 1-6) mutations in keloids].
abstract: OBJECTIVE
  To detect Fas DNA mutations (exon 1-6) in the fibroblasts of patients with keloids, thereby to understand the clinicopathological implications of altered structure of the keloids.
  
  
  METHODS
  PCR followed by single-strand conformational polymorphism analysis and direct DNA sequencing were used to detect Fas gene mutations in 15 patients with keloids.
  
  
  RESULTS
  Insertion and point mutations were identified on the boundary between intron 5 and exon 6 in two patients, while no Fas mutations were found in the fibroblasts derived from normal skin samples of any of the patients.
  
  
  CONCLUSIONS
  Nonfunction of Fas protein may be related to aberrant gene structure that codes for the transmembrane domain.
authors.affiliation: Department of Plastic Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Yong-Bo
authors.initials: YB
authors.lastname: Liu
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Gao
authors.forename: Hong-Jie
authors.initials: HJ
authors.lastname: Duan
mesh_terms: D000328:Adult; D004247:DNA / Q000032:analysis; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D007627:Keloid / Q000235:genetics*; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D019014:fas Receptor / Q000032:analysis / Q000235:genetics*
chemical_list: D019014:fas Receptor; D004247:DNA