Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation.
A large number of hDAF transgenic pigs to be used for xenotransplantation research were generated by using sperm-mediated gene transfer (SMGT). The efficiency of transgenesis obtained with SMGT was much greater than with any other method. In the experiments reported, up to 80% of pigs had the transgene integrated into the genome. Most of the pigs carrying the hDAF gene transcribed it in a stable manner (64%). The great majority of pigs that transcribed the gene expressed the protein (83%). The hDAF gene was transmitted to progeny. Expression was stable and found in caveolae as it is in human cells. The expressed gene was functional based on in vitro experiments performed on peripheral blood mononuclear cells. These results show that our SMGT approach to transgenesis provides an efficient procedure for studies involving large animal models.
Lavitrano
Marialuisa
M
Dipartimento di Medicina Sperimentale, Università La Sapienza, 00161 Roma, Italy. marialuisa.lavitrano@unibo.it
Bacci
Maria Laura
ML
Forni
Monica
M
Lazzereschi
Davide
D
Di Stefano
Carla
C
Fioretti
Daniela
D
Giancotti
Paola
P
Marfé
Gabriella
G
Pucci
Loredana
L
Renzi
Luigina
L
Wang
Hongjun
H
Stoppacciaro
Antonella
A
Stassi
Giorgio
G
Sargiacomo
Massimo
M
Sinibaldi
Paola
P
Turchi
Valeria
V
Giovannoni
Roberto
R
Della Casa
Giacinto
G
Seren
Eraldo
E
Rossi
Giancarlo
G
D000818:Animals; D030801:Animals, Genetically Modified; D018958:CD55 Antigens / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D018014:Gene Transfer Techniques; D006801:Humans; D008297:Male; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine; D019076:Transgenes; D014183:Transplantation, Heterologous
D018958:CD55 Antigens; D004247:DNA