Isoforms and single nucleotide polymorphisms of the FSH receptor gene: implications for human reproduction.
The FSH receptor shows three single nucleotide polymorphisms (SNPs), one in the promoter and two in exon 10. In addition, the FSH receptor mRNA undergoes extensive alternative splicing. While no physiological role for the SNP in the promoter and for alternative spliced isoforms has been demonstrated so far, the SNPs in exon 10 result in four discrete allelic variants characterized by the amino acid combinations Thr307-Asn680, Ala307-Ser680, Ala307-Asn680 and Thr307-Ser680. Several studies have demonstrated that the first two allelic variants are very frequent (approximately 60 and 40% respectively) in the Caucasian population. The rarer Ala307-Asn680 and Thr307-Ser680 variants are much less frequent (<5%) in the Chinese. In males the FSH receptor variants are not related to testicular volume, serum FSH or serum inhibin B levels. The two most common receptor variants transiently transfected in COS-7 cells displayed similar functional characteristics. Frequency distribution of the two polymorphisms in normal women and patients with polycystic ovarian syndrome or premature ovarian failure is still under investigation. The homozygous Ala307-Ser680 variant seems to be associated with significantly higher basal serum FSH levels and with a higher amount of FSH required for ovarian stimulation in women undergoing assisted reproduction. This suggests that the FSH receptor genotype can influence the ovarian response to FSH stimulation. The presence of SNPs in the FSH receptor gene capable of modifying FSH action paves the way for future patient-tailored, genotype-based hormone therapies.
Simoni
M
M
Institute of Reproductive Medicine of the University, Münster, Germany. simoni@uni-muenster.de
Nieschlag
E
E
Gromoll
J
J
D000483:Alleles; D000818:Animals; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D009154:Mutation; D010053:Ovary / Q000502:physiology; D011110:Polymorphism, Genetic / Q000502:physiology; D020641:Polymorphism, Single Nucleotide; D020033:Protein Isoforms / Q000502:physiology; D011962:Receptors, FSH / Q000235:genetics* / Q000502:physiology; D012098:Reproduction / Q000502:physiology*; D013737:Testis / Q000502:physiology
D020033:Protein Isoforms; D011962:Receptors, FSH