title: Rapid HPLC measurement of buflomedil in plasma in poisoning cases.
abstract: A rapid high-performance liquid chromatographic method for the determination of buflomedil in human plasma is described. It requires a single liquid-liquid extraction step from 1 mL of plasma with diethyl ether followed by chromatography on a Nova Pak C(18) reversed-phase column and detection by ultaviolet light. Metoclopramide was used as internal standard. The method is sensitive with a quantification limit at 500 ng/mL. It was used for the determination of buflomedil in biological fluids in poisoning cases.
authors.affiliation: EA2962-Pharmacochimie, Université Victor Segalen Bordeaux 2, Bordeaux, France. isabelle.bares@chimphys.u-bordeaux2.fr
authors.forename: I
authors.initials: I
authors.lastname: Forfar-Bares
authors.forename: F
authors.initials: F
authors.lastname: Pehourcq
authors.forename: C
authors.initials: C
authors.lastname: Jarry
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011041:Poisoning / Q000097:blood*; D011759:Pyrrolidines / Q000097:blood* / Q000506:poisoning; D012015:Reference Standards; D015203:Reproducibility of Results; D014665:Vasodilator Agents / Q000097:blood* / Q000506:poisoning
chemical_list: D011759:Pyrrolidines; D014665:Vasodilator Agents; C010651:buflomedil