Angiotensin converting enzyme DD genotype is associated with hypertensive crisis.
OBJECTIVE
The genetic background of hypertensive crisis is unknown. We examined the association of polymorphisms in genes involved in the renin-angiotensin-aldosterone-system with hypertensive crisis.


METHODS
Population-based case-control study.


METHODS
Emergency department at a tertiary care university hospital.


METHODS
A total of 182 patients with essential hypertension who were admitted to an emergency department for treatment of hypertensive crisis and 182 age- and sex-matched healthy individuals.


METHODS
None.


METHODS
Analysis of polymorphisms in genes coding for angiotensinogen (AJT 704T-->C), angiotensin II receptor 1 (AGTR1 1166A-->C), renin (REN 2646G-->A), renin-binding protein (RENBP 61T-->C), alpha-adducin (ADD1 1378G-->T), beta-2-adrenergic receptor (ADRB2 46A-->G, 79C-->G), and angiotensin I converting enzyme (ACE I/D) was performed by polymerase chain reaction and restriction fragment length polymorphism analysis. MAIN RESULTS Among patients, the ACE I/D polymorphism showed a deviation from Hardy-Weinberg equilibrium (p =.01). In controls, all polymorphisms were in the Hardy-Weinberg equilibrium. The frequency of the DD genotype was increased in patients (n = 70, 38.5%) vs. controls (n = 51; 28.0%;p =.03; odds ratio, 1.61; 95% confidence interval, 1.03-2.50), which was due to the DD genotype in 40 male patients (44%) vs. 23 in male controls (25.3%;p =.004; odds ratio, 3.48; 95% confidence interval, 1.47-8.30). There were no differences in genotype distributions among other polymorphisms.


CONCLUSIONS
We demonstrate a possible association of the DD genotype with hypertensive crisis in men.
Sunder-Plassmann
Gere
G
Department of Medicine III, University of Vienna, Austria.
Kittler
Harald
H
Eberle
Corinna
C
Hirschl
Michael M
MM
Woisetschläger
Christian
C
Derhaschnig
Ulla
U
Laggner
Anton N
AN
Hörl
Walter H
WH
Födinger
Manuela
M
D000808:Angiotensinogen / Q000235:genetics; D002148:Calmodulin-Binding Proteins / Q000235:genetics; D002238:Carbohydrate Epimerases / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D016022:Case-Control Studies; D004630:Emergencies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D044140:Receptor, Angiotensin, Type 1; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics; D011945:Receptors, Angiotensin / Q000235:genetics; D012083:Renin / Q000235:genetics; D012084:Renin-Angiotensin System / Q000235:genetics*; D000099291:Adducins
D000808:Angiotensinogen; D002148:Calmodulin-Binding Proteins; D002238:Carbohydrate Epimerases; D002352:Carrier Proteins; D007703:Peptidyl-Dipeptidase A; D044140:Receptor, Angiotensin, Type 1; D018343:Receptors, Adrenergic, beta-2; D011945:Receptors, Angiotensin; D012083:Renin; D000099291:Adducins; C024519:N-acyl-D-glucosamine 2-epimerase; C498259:RENBP protein, human