title: CYP92B1, A cytochrome P450, expressed in petunia flower buds, that catalyzes monooxidation of long-chain fatty acids.
abstract: In higher plants, long-chain fatty acid hydroperoxides are intermediates in the synthesis of a diverse group of bioactive compounds. We used the reverse trascriptase-polymerase chain reaction to isolate a gene responsible for the oxidization of fatty acids from Petunia hybrida. A P450 cDNA not isolated earlier, CYP92B1, contained an open reading frame predicted to encode a polypeptide consisting of 510 amino acid residues. The transcript of the cyp92B1 gene was expressed at a high level in the early stage of flower development. CYP92B1 cDNA was expressed in a yeast, Saccharomyces cerevisiae, and recombinant yeast microsomes containing CYP92B1, a hemoprotein, metabolized lauric acid, linoleic acid, and linolenic acid.
authors.affiliation: Research Center for Environmental Genomics, Kobe University, Japan.
authors.forename: Mariana
authors.initials: M
authors.lastname: Petkova-Andonova
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Imaishi
authors.forename: Hideo
authors.initials: H
authors.lastname: Ohkawa
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002384:Catalysis; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry* / Q000378:metabolism*; D035264:Flowers / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D007850:Lauric Acids / Q000378:metabolism; D019787:Linoleic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D032306:Petunia / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D015003:Yeasts / Q000235:genetics / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D005227:Fatty Acids; D007850:Lauric Acids; D017962:alpha-Linolenic Acid; C030358:lauric acid; D003577:Cytochrome P-450 Enzyme System; D019787:Linoleic Acid