Effects of prostaglandin E2 on gastric irritant-induced apoptosis.
We previously reported that various gastric irritants induced both apoptosis and necrosis in cultured gastric mucosal cells. In a continuation of this work, the present study has examined the effects of prostaglandin E2 (PGE2), a cytoprotective factor for gastric mucosa in vivo, on gastric irritant-induced apoptosis and necrosis in vitro. PGE2 inhibited ethanol-induced apoptosis and increased cell viability in a dose-dependent manner in primary cultures of guinea pig gastric mucosal cells. PGE2 also inhibited hydrogen peroxide-induced apoptosis. In contrast, PGE2 showed no cytoprotective effects against ethanol-induced necrosis. Based on these results, we consider that the cytoprotective effects of PGE2 on gastric mucosa in vivo can be partially explained by its inhibitory effect on gastric irritant-induced apoptosis.
Hoshino
Tatsuya
T
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Takano
Tatsunori
T
Tsutsumi
Shinji
S
Tomisato
Wataru
W
Tsuchiya
Tomofusa
T
Mizushima
Tohru
T
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D006168:Guinea Pigs; D006851:Hydrochloric Acid / Q000633:toxicity; D006861:Hydrogen Peroxide / Q000633:toxicity; D066298:In Vitro Techniques; D008297:Male; D009336:Necrosis
D000431:Ethanol; D006861:Hydrogen Peroxide; D015232:Dinoprostone; D006851:Hydrochloric Acid