Culture conditions dictate protease and tannase production in submerged and solid-state cultures of Aspergillus niger Aa-20.
Undesirable protease production by Aspergillus niger Aa-20 in submerged culture and solid-state culture was evaluated using different concentrations of tannic acid as sole carbon source in a model system designed for tannase production. Protease production was found to be dependent on the culture system used (submerged culture or solid-state culture) and on the initial tannic acid concentration. Expression of protease activity in submerged culture was higher (up to 10 times) than activity obtained in solid-state culture, using identical culture medium composition. In submerged culture, the lowest final protease activity (0.13 IU) was obtained with the highest tannic acid concentration, while in solid-state culture protease activity was not affected by changes in initial substrate concentration. Absence of detectable proteolytic activity in solid-state culture is related to high production of tannase enzyme. Hence, the use of solid-state culture for fungal enzyme production may allow for higher and more stable enzyme titers present in culture extracts.
Aguilar
Cristóbal Noé
CN
Food Research Department, Faculty of Chemical Sciences, Universidad Autónoma de Coahuila, Unidad Saltillo, México.
Favela-Torres
Ernesto
E
Viniegra-González
Gustavo
G
Augur
Christopher
C
D001234:Aspergillus niger / Q000187:drug effects / Q000201:enzymology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D003470:Culture Media; D010450:Endopeptidases / Q000096:biosynthesis* / Q000378:metabolism; D004795:Enzyme Stability; D047348:Hydrolyzable Tannins / Q000378:metabolism* / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D013997:Time Factors
D003470:Culture Media; D047348:Hydrolyzable Tannins; D011480:Protease Inhibitors; D002265:Carboxylic Ester Hydrolases; C022711:tannase; D010450:Endopeptidases