A new collagen from the extracellular matrix of Sepia officinalis cartilage.
Guanidinium chloride treatment of Sepia officinalis cartilage solubilized a component that contained hydroxyproline. Electron-microscopy observation of rotary-shadowed preparations of this component revealed it to consist of rod-like units themselves consisting of filaments. Dialysis of an acetic acid solution against ATP afforded polymeric aggregates consisting of a succession of two or three thick sections showing transverse electron-opaque banding, separated by thinner sections without banding. Electrophoresis produced a main band of about 140 kDa sensitive to bacterial collagenase. After reduction with mercaptoethanol, electrophoresis afforded a 40-kDa band. Pepsin digestion resulted in additional electrophoretic bands. These data suggest the presence of a collagen in Sepia cartilage with characteristics unlike those of any known collagen.
Rigo
Cristina
C
Dipartimento di Fisiologia e Biochimica Generali, Sezione di Istologia ed Anatomia Umana, Università degli Studi di Milano, via Celoria 26, 20133 Milan, Italy. rigo@mailserver.unimi.it
Bairati
Aurelio
A
D019342:Acetic Acid / Q000737:chemistry; D000818:Animals; D001704:Biopolymers / Q000737:chemistry* / Q000378:metabolism; D002356:Cartilage / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D017364:Collagenases / Q000378:metabolism; D003956:Dialysis; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D019791:Guanidine / Q000494:pharmacology; D006909:Hydroxyproline / Q000032:analysis; D008623:Mercaptoethanol / Q000737:chemistry; D008970:Molecular Weight; D008974:Mollusca / Q000737:chemistry*; D010084:Oxidation-Reduction; D010434:Pepsin A / Q000737:chemistry
D001704:Biopolymers; D008623:Mercaptoethanol; D003094:Collagen; D010434:Pepsin A; D017364:Collagenases; D019791:Guanidine; D019342:Acetic Acid; D006909:Hydroxyproline