title: Cloning and characterisation of a procorticotrophin-releasing hormone in the IZD-MB-0503 immunocyte line from the insect Mamestra brassicae.
abstract: The cloning and characterisation of a procorticotrophin-releasing hormone (proCRH) and the related CRH fragment in the IZD-MB-0503 cell line from the leptidopteran Mamestra brassicae were performed. PCR amplification of the genomic DNA reveals a fragment of 276 bp, while inverse PCR shows the presence of a gene consisting of 1153 bp. The comparison of the insect genomic proCRH gene with proCRH cDNA obtained by RACE shows the presence of three introns. There was a 61% identity with the corresponding coding sequence in Tilapia mossambica, and a 65.2% identity with the human proCRH coding sequence.
authors.affiliation: Department of Animal Biology, University of Modena and Reggio Emilia, via Campi, 213/D-41100 Modena, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Malagoli
authors.forename: Mauro
authors.initials: M
authors.lastname: Mandrioli
authors.forename: Enzo
authors.initials: E
authors.lastname: Ottaviani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003346:Corticotropin-Releasing Hormone / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics*; D007915:Lepidoptera / Q000166:cytology / Q000235:genetics*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D019476:Insect Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D003346:Corticotropin-Releasing Hormone