A new method for determination of buformin in plasma and urine by ion-paired reversed-phase HPLC with ultraviolet detection.
Buformin is a widely used as an antidiabetic agent but its renal excretion is still controversial. A new HPLC method with ultraviolet (UV) detection for the determination of buformin in plasma and urine has been developed. After protein precipitation or dilution, buformin and internal standard phenformin were resolved on an octadecyl silica column and detected by UV detection at 233 nm. Intra- and inter-day coefficients of variation were <9%. The limit of quantification was around 0.05 micro g/ml for plasma and 2.5 micro g/ml for urine.
Yamamoto
Takehito
T
Department of Pharmacy, The University of Tokyo Hospital, Faculty of Medicine, The University of Tokyo, 3-1 Hongo 7-chome, Bunkyo-ku, Tokyo 113-8655, Japan.
Kusama
Makiko
M
Matsuno
Kumi
K
Sugiyama
Erika
E
Yamada
Yasuhiko
Y
Iga
Tatsuji
T
D002026:Buformin / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D007004:Hypoglycemic Agents; D002026:Buformin