[Effects of androgen and estrogen on the expressions of basic fibroblast growth factor, transforming growth factor and smoothelin].
OBJECTIVE
To study effects of androgen and estrogen on the expressions of basic fibroblast growth factor (bFGF) and transforming growth factor beta2 (TGFbeta2) in cultured human prostatic stromal cells.


METHODS
Human prostatic stromal cells obtained from 11 patients with benign prostatic hypertrophy (BPH) were cultured and stimulated with dihydrotestosterone (DHT) and estradiol (E2). The expressions of bFGF and TGFbeta2 mRNA along with smoothelin mRNA were observed with reverse transcriptase-polymerase chain reaction.


RESULTS
DHT significantly upregulated bFGF expression, and E2 enhanced TGFbeta2 and smoothelin expressions. A positive correlation between expressions of TGFbeta2 and smoothelin was observed.


CONCLUSIONS
DHT can induce bFGF expression and E2 promotes TGFbeta2 expression, and the transformation toward smooth muscle cells induced by E(2) may involve the action of TGFbeta2.
Tang
Wei
W
Department of Urology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Zheng
Shao-Bin
SB
Zhang
Jin-Hua
JH
D003598:Cytoskeletal Proteins / Q000096:biosynthesis; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis; D011467:Prostate / Q000166:cytology; D017154:Stromal Cells / Q000187:drug effects* / Q000378:metabolism; D015291:Transforming Growth Factors / Q000096:biosynthesis*; D015854:Up-Regulation
D003598:Cytoskeletal Proteins; D009124:Muscle Proteins; C100762:SMTN protein, human; D013196:Dihydrotestosterone; D016222:Fibroblast Growth Factor 2; D004958:Estradiol; D015291:Transforming Growth Factors