title: Diagnostic evidence for the presence of beta-agonists using two consecutive derivatization procedures and gas chromatography-mass spectrometric analysis.
abstract: A GC-MS procedure for the detection of different beta-agonists in urine samples based on two consecutive derivatization steps is described. The derivatization procedure is based on the consecutive formation of cyclic methylboronate derivatives followed by a second derivatization step with MSTFA on the same extract, forming TMS derivatives. Injections in the GC-MS system may be carried out after each one of the derivatization steps, obtaining enough information for unambiguous identification. Limits of detection for the two derivatization steps ranged from 0.5 to 5 ng/ml. This procedure was tested with the beta-agonists bambuterol, clenbuterol, fenoterol, formoterol, salbutamol, salmeterol, alpha-hydroxy-salmeterol and terbutaline.
authors.affiliation: Unitat de Farmacologia, Institut Municipal d'Investigació Mèdica, Barcelona, Spain.
authors.forename: Lúcia
authors.initials: L
authors.lastname: Damasceno
authors.forename: Rosa
authors.initials: R
authors.lastname: Ventura
authors.forename: Jari
authors.initials: J
authors.lastname: Cardoso
authors.forename: Jordi
authors.initials: J
authors.lastname: Segura
mesh_terms: D000318:Adrenergic beta-Agonists / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000318:Adrenergic beta-Agonists