lal-1: a differentially expressed novel gene during proliferation in liver regeneration and in hepatoma cells.
BACKGROUND
During liver regeneration, 95% of the resting hepatocytes enter in G1/S phase of the cell cycle. A number of hormones, growth factors and cytokines were identified that activate signal transduction pathways playing a primary role in hepatocyte proliferation. A wide and representative cDNA library containing 1.5 x 106 independent clones was constructed from regenerating liver in order to identify and characterize gene the products which play a crucial role in the first hours of the proliferative process of liver regeneration.


RESULTS
A novel gene expressed in liver regeneration was cloned by subtractive hybridization. The putative protein displays in the N'-terminal a annexin-like domain and an aminopeptidase domain. We named the novel gene Liver Annexin Like-1 (lal-1). The lal-1 gene is modulated during liver regeneration, in hepatoma cells following physiological stimulation and after cAMP induction.


CONCLUSIONS
The results indicate that lal-1 is involved in liver regeneration and that its expression is finely regulated during proliferative process. The isolation of lal-1 paves the way for a further characterization helping to assess lal-1 involvement in cell function and proliferation.
Della Fazia
Maria Agnese
MA
Dipartimento di Scienze Biochimiche e Biotecnologie Molecolari, Sezione Fisiopatologia--Facoltà di Medicina e Chirurgia-Università di Perugia, 06100 Perugia, Italy.
Piobbico
Danilo
D
Bartoli
Daniela
D
Castelli
Marilena
M
Brancorsini
Stefano
S
Viola Magni
Mariapia
M
Servillo
Giuseppe
G
D000818:Animals; D017302:Annexins / Q000235:genetics* / Q000502:physiology; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D008115:Liver Regeneration / Q000235:genetics* / Q000502:physiology; D008856:Microscopy, Fluorescence; D009928:Organ Specificity; D051381:Rats; D017422:Sequence Analysis, DNA
D017302:Annexins; C473753:Cpq protein, rat; D004273:DNA, Neoplasm