Analysis of surface properties of human cancer cells using derivatized beads.
Standard histochemical analysis of cells and tissues generally involves procedures that utilize a relatively small number of probes such as dyes, and generally requires hours or days to process. Our laboratory has developed a novel method for histochemical surveys of cell surface properties that utilizes a large number of probes (derivatized agarose beads) and takes seconds or minutes to accomplish. In this study, 4 human cell lines (CCL-255 (LS123) human colon cancer cells that are non-tumorigenic in nude mice; CRL-1459 (CCD-18CO) human colon endothelial cells that are non-malignant; CCL-220 (COLO 320DM) human colon cancer cells that are tumorigenic in nude mice; and HTB-171 (NCI H446) human lung carcinoma cells) were tested for their ability to bind to agarose beads derivatized with 51 different molecules. There were statistically significant differences in binding of the 4 cell types to all of the 51 types of beads, but 15 types of beads showed dramatic differences in binding to one or more of the 4 cell types. For example, only HTB-171 (NCI H446) bound to p-aminophenyl-beta-D-glucopyranoside-derivatized beads and only CCL-220 (COLO 320DM) bound to L-tyrosine-derivatized beads. The specificity of cell-bead binding was examined by performing assays in the presence or absence of exogenously added compounds in hapten-type of inhibition experiments. This assay, that utilizes large numbers of novel probes, may help in the development of new libraries of surface properties of specific cell types, with differing degrees of malignancy, that at this time could not be developed by using other available technologies.
Khurrum
Maria R
MR
Department of Biology and Center for Cancer and Developmental Biology, California State University, Northridge 91330-8303, USA.
Weerasinghe
Gayani R
GR
Soriano
Evelyn S
ES
Riman
Rashad
R
Badali
Oliver
O
Gipson
Stephanie
S
Medina
Jessica
J
Alfaro
Juan
J
Navarro
Vanessa M
VM
Harieg
Caroline B
CB
Ngo
Lylla
L
Sakhakorn
Tharinee
T
Kirszenbaum
Lital
L
Khatibi
David
D
Abedi
Karolin
K
Barajas
Marcela
M
Zem
Gregory C
GC
Kirszenbaum
Adit
A
Razi
Arash
A
Oppenheimer
Steven B
SB
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D054418:Chemokine CCL20; D019742:Chemokines, CC / Q000737:chemistry / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D006639:Histidine / Q000737:chemistry; D006651:Histocytochemistry; D006801:Humans; D019402:Macrophage Inflammatory Proteins / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008863:Microspheres; D054399:Receptors, CCR6; D019707:Receptors, Chemokine; D012685:Sepharose / Q000737:chemistry; D014407:Tumor Cells, Cultured
C108355:CCL20 protein, human; C106311:CCR6 protein, human; D054418:Chemokine CCL20; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D054399:Receptors, CCR6; D019707:Receptors, Chemokine; D003208:Concanavalin A; D006639:Histidine; D012685:Sepharose; D001120:Arginine