Immunocytochemical localization of substance P receptor in hypothalamic oxytocin-containing neurons of C57 mice.
With the use of double immunofluorescence, we have examined the distribution of oxytocin-containing neurons that express substance P receptor (SPR) in the hypothalamus of C57 mice. The distribution of oxytocin-like immunoreactive neurons overlapped with that of SPR-like immunoreactive neurons in the paraventricular nucleus and supraoptic nucleus of the hypothalamus. Neurons showing both oxytocin- and SPR-like immunoreactivities were predominantly found in both nuclei. A few neurons that were double-labeled with oxytocin- and SPR-like immunoreactivities were also scattered in the hypothalamic periventricular and preoptic regions. Semi-quantitative analysis indicated that about 94% of the oxytocin-like neurons displayed SPR-like immunoreactivity. These double-labeled cells constituted about 91% of the SPR-like neurons in the aforementioned regions. The present study provides morphological evidence for tachykinin-induced modulation of oxytocin-containing neurons as mediated by substance P receptor in the hypothalamus of mammals.
Liu
Hui-Ling
HL
Institute of Neurosciences, The Fourth Military Medical University, Xi'an 710032, PR China.
Cao
Rong
R
Jin
Liang
L
Chen
Liang-Wei
LW
D000818:Animals; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010121:Oxytocin / Q000096:biosynthesis*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000378:metabolism; D018040:Receptors, Neurokinin-1 / Q000096:biosynthesis*; D013495:Supraoptic Nucleus / Q000166:cytology / Q000378:metabolism
D018040:Receptors, Neurokinin-1; D010121:Oxytocin