Surface topography of hydroxyapatite affects ROS17/2.8 cells response.
Hydroxyapatite (HA) has been used in orthopedic, dental, and maxillofacial surgery as a bone substitute. The aim of this investigation was to study the effect of surface topography produced by the presence of microporosity on cell response, evaluating: cell attachment, cell morphology, cell proliferation, total protein content, and alkaline phosphatase (ALP) activity. HA discs with different percentages of microporosity (< 5%, 15%, and 30%) were confected by means of the combination of uniaxial powder pressing and different sintering conditions. ROS17/2.8 cells were cultured on HA discs. For the evaluation of attachment, cells were cultured for two hours. Cell morphology was evaluated after seven days. After seven and fourteen days, cell proliferation, total protein content, and ALP activity were measured. Data were compared by means of ANOVA and Duncan's multiple range test, when appropriate. Cell attachment (p = 0.11) and total protein content (p = 0.31) were not affected by surface topography. Proliferation after 7 and 14 days (p = 0.0007 and p = 0.003, respectively), and ALP activity (p = 0.0007) were both significantly decreased by the most irregular surface (HA30). These results suggest that initial cell events were not affected by surface topography, while surfaces with more regular topography, as those present in HA with 15% or less of microporosity, favored intermediary and final events such as cell proliferation and ALP activity.
Rosa
Adalberto Luiz
AL
Department of Surgery, Maxillofacial Traumatology and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Brasil.
Beloti
Márcio Mateus
MM
Van Noort
Richard
R
Hatton
Paul Vincent
PV
Devlin
Anne Jane
AJ
D000469:Alkaline Phosphatase / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002455:Cell Division; D048429:Cell Size; D017886:Durapatite; D016348:Osseointegration; D010006:Osteoblasts / Q000502:physiology*; D016062:Porosity
D001672:Biocompatible Materials; D017886:Durapatite; D000469:Alkaline Phosphatase