DNA strand exchange stimulated by spontaneous complex formation with cationic comb-type copolymer.
Cationic comb-type copolymers (CCCs) composed of a polycation backbone and water-soluble side chains accelerate by 4-5 orders the DNA strand exchange reaction (SER) between double helical DNA and its homologous single-strand DNA. The accelerating effect is considered due to alleviation of counterion association during transitional intermediate formation in sequential displacement pathway. CCCs stabilize not only matured hybrids but also the nucleation complex to accelerate hybridization.
Kim
Won Jong
WJ
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, and Presto, Japan Science and Technology Corporation, Midori, Yokohama 226-8501, Japan.
Akaike
Toshihiro
T
Maruyama
Atsushi
A
D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D003911:Dextrans / Q000737:chemistry; D004735:Energy Transfer; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes; D007700:Kinetics; D011107:Polylysine / Q000737:chemistry; D013816:Thermodynamics
D002412:Cations; D004277:DNA, Single-Stranded; D003911:Dextrans; D005456:Fluorescent Dyes; D011107:Polylysine; D004247:DNA; D016650:Fluorescein-5-isothiocyanate