Reconstruction of glycosaminoglycan chains in decorin.
The glycosaminoglycan chain of decorin from human spinal ligaments was digested using the hydrolysis of bovine testicular hyaluronidase. As a result, decorin with hexasaccharide, octasaccharide, and decasaccharide including the linkage region, GlcA-Gal-Gal-Xyl, was obtained. The obtained decorin as an acceptor and hyaluronic acid as a donor were incubated with bovine testicular hyaluronidase under the condition of transglycosylation reaction. The transglycosylation reaction product had hexasaccharide to triacontasaccharide. Judging from the analysis of glycosaminoglycan chain in the transglycosylation reaction product, it was confirmed that hyaluronic acid chain as a donor was transferred to the retained glycosaminoglycan chain of decorin as an acceptor. Similarly, it was possible to reconstruct the glycosaminoglycan chain in decorin to chondroitin, chondroitin 4-sulfate or chondroitin 6-sulfate. Therefore, we succeeded in synthesizing an artificial family of decorins.
Iwafune
Mito
M
Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, 036-8562, Hirosaki, Japan. mito29@c.hirosaki-u.ac.jp
Kakizaki
Ikuko
I
Yukawa
Masahiro
M
Kudo
Daisuke
D
Ota
Sakae
S
Endo
Masahiko
M
Takagaki
Keiichi
K
D000818:Animals; D002417:Cattle; D002807:Chondroitin / Q000737:chemistry; D002809:Chondroitin Sulfates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005690:Galactose / Q000737:chemistry; D006025:Glycosaminoglycans / Q000737:chemistry*; D006031:Glycosylation; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D006868:Hydrolysis; D007118:Immunoassay; D011509:Proteoglycans / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013131:Spine / Q000378:metabolism; D013997:Time Factors
C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; D011509:Proteoglycans; D002807:Chondroitin; D002809:Chondroitin Sulfates; D006821:Hyaluronoglucosaminidase; D005690:Galactose