Characterization of nucleic acids by nanopore analysis.
Single-stranded DNA and RNA molecules in solution can be driven through a nanoscopic pore by an applied electric field. As each molecule occupies the pore, a characteristic blockade of ionic current is produced. Information about length, composition, structure, and dynamic motion of the molecule can be deduced from modulations of the current blockade.
Deamer
David W
DW
Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA. deamer@hydrogen.ucsc.edu
Branton
Daniel
D
D001704:Biopolymers / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D004553:Electric Conductivity; D004563:Electrochemistry / Q000379:methods*; D006460:Hemolysin Proteins / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D013696:Temperature
D001704:Biopolymers; D004277:DNA, Single-Stranded; D006460:Hemolysin Proteins; D009696:Nucleic Acids