title: The alpha(2)beta(1) and alpha(3)beta(1) integrins do not mediate attachment of endometrial cells to peritoneal mesothelium.
abstract: OBJECTIVE
  To evaluate the possible role of mesothelial alpha(2)beta(1) and alpha(3)beta(1) integrins in the attachment of endometrial stromal cells (ESCs) and endometrial epithelial cells (EECs).
  
  
  METHODS
  In vitro study.
  
  
  METHODS
  University medical center.
  
  
  METHODS
  Women of reproductive age (n = 26).
  
  
  METHODS
  Mesothelial cells were grown on collagen IV. Endometrial stromal cells and EECs were plated on mesothelial cells for 1 hour. Before plating, mesothelial cells or endometrial cells were incubated with antibodies to alpha2, alpha3, and beta1 integrin subunits. The effect of these antibodies on ESC and EEC binding to collagen IV and collagen I was also examined. The expression of collagen I, collagen IV, fibronectin, and laminin by cultured ESCs and EECs was examined.
  
  
  RESULTS
  The anti-integrin antibodies had no effect on endometrial binding to mesothelium. The beta1 integrin antibody decreased binding of ESCs and EECs to the collagen matrices. In culture, ESCs and EECs expressed collagen I, collagen IV, fibronectin, and laminin to varying degrees.
  
  
  CONCLUSIONS
  The initial adhesion of ESCs and EECs to mesothelium is not mediated by beta1 integrins. In contrast, ESC and EEC attachment to collagen IV and collagen I, which are present in the submesothelial extracellular matrix, is mediated by beta1 integrins.
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, Texas, San Antonio 78229-3900, USA. witz@uthscsa.edu
authors.forename: Craig A
authors.initials: CA
authors.lastname: Witz
authors.forename: Sook
authors.initials: S
authors.lastname: Cho
authors.forename: Iris A
authors.initials: IA
authors.lastname: Montoya-Rodriguez
authors.forename: Robert S
authors.initials: RS
authors.lastname: Schenken
mesh_terms: D000328:Adult; D000906:Antibodies / Q000494:pharmacology; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D024042:Collagen Type I / Q000032:analysis / Q000378:metabolism; D024141:Collagen Type IV / Q000032:analysis / Q000378:metabolism; D003470:Culture Media; D004717:Endometrium / Q000166:cytology*; D004848:Epithelium / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D038982:Integrin alpha2beta1 / Q000276:immunology / Q000502:physiology*; D039201:Integrin alpha3beta1 / Q000276:immunology / Q000502:physiology*; D007797:Laminin / Q000032:analysis; D010537:Peritoneum / Q000166:cytology*
chemical_list: D000906:Antibodies; D024042:Collagen Type I; D024141:Collagen Type IV; D003470:Culture Media; D005353:Fibronectins; D038982:Integrin alpha2beta1; D039201:Integrin alpha3beta1; D007797:Laminin