Direct in vitro effects of androgens and estrogens on ob gene expression and leptin secretion in human adipose tissue.
In the present study, we have explored, in vitro, the possibility that short exposure to androgens and estrogens for 24 h may directly influence leptin expression (ARNm and secretion) in sc adipose tissue from men and women. In men, only dihydrotestosterone at high concentration (100 nM) induced a reduction in leptin secretion and ob mRNA level. In women, 17beta-estradiol (10-100 nM) increased ob mRNA expression (+180 to +500%) and leptin release (+75%). Moreover, in adipose tissue of women, the estrogen precursors testosterone (100 nM) and dehydroepiandrosterone (1 microM) also induced an increase in leptin secretion (+84 and +96%, respectively), an effect that was prevented by the aromatase inhibitor letrozole. Finally, the stimulatory effect of 17beta-estradiol observed in women was antagonized by the antiestrogen ICI182780. Altogether, these results suggest that the sexual dimorphism of leptinemia in humans is mainly owing to the estrogen receptor-dependent stimulation of leptin expression in adipose tissue by estrogens and estrogen precursors in women.
Machinal-Quélin
Florence
F
Service de Biochimie et de Biologie Moléculaire, Faculté de Médecine Paris-Ouest, Université René Descartes (Paris V), Centre Hospitalier de Poissy, France.
Dieudonné
Marie-Noëlle
MN
Pecquery
René
R
Leneveu
Marie-Christine
MC
Giudicelli
Yves
Y
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D000328:Adult; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D012739:Gonadal Steroid Hormones / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D020738:Leptin / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D013739:Testosterone / Q000494:pharmacology
D012739:Gonadal Steroid Hormones; D020738:Leptin; D012333:RNA, Messenger; D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol