Incorporation of large heterologies into heteroduplex DNA during double-strand-break repair in mouse cells.
In this study, the formation and repair of large (>1 kb) insertion/deletion (I/D) heterologies during double-strand-break repair (DSBR) was investigated using a gene-targeting assay that permits efficient recovery of sequence insertion events at the haploid chromosomal immunoglobulin (Ig) mu-locus in mouse hybridoma cells. The results revealed that (i) large I/D heterologies were generated on one or both sides of the DSB and, in some cases, formed symmetrically in both homology regions; (ii) large I/D heterologies did not negatively affect the gene targeting frequency; and (iii) prior to DNA replication, the large I/D heterologies were rectified.
Raynard
Steven J
SJ
Department of Molecular Biology and Genetics, College of Biological Science, University of Guelph, Ontario, Canada.
Baker
Mark D
MD
D000818:Animals; D004249:DNA Damage / Q000502:physiology*; D004260:DNA Repair / Q000502:physiology*; D005819:Genetic Markers; D020180:Heteroduplex Analysis / Q000379:methods*; D051379:Mice; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics*; D015003:Yeasts / Q000235:genetics
D005819:Genetic Markers; D009692:Nucleic Acid Heteroduplexes