Use of the post-insertion method for the formation of ligand-coupled liposomes.
A new technique is described for the formation of ligand-targeted liposomes that can be used with whole antibodies, antibody fragments, peptides or other ligands. The ligands are coupled to polyethylene glycol micelles and then transferred in a simple incubation step from the micelles into the outer monolayer of pre-formed, drug-loaded liposomes. This versatile method allows a combinatorial approach to the design of targeted liposomes that minimises manufacturing complexities, allowing a variety of ligands to be inserted into a variety of pre-formed liposomes containing a variety of drugs. This allows the ligand-targeted therapeutics to be tailored to the needs of individual patients.
Allen
Theresa M
TM
Department of Pharmacology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.
Sapra
Puja
P
Moase
Elaine
E
D016503:Drug Delivery Systems; D008024:Ligands; D008081:Liposomes / Q000737:chemistry*; D008823:Micelles; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011092:Polyethylene Glycols
D008024:Ligands; D008081:Liposomes; D008823:Micelles; D010714:Phosphatidylethanolamines; C038089:1,2-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols