[DNA, RNA and hemoglobin extraction from one sample of peripheral blood].
Nucleated cells were isolated from peripheral blood of healthy normal adults, dissolved and centrifuged to acquire the supernatant for RNA and the deposit for DNA extraction. Hemoglobin was extracted from the deposit formed by the red blood cells. Property assessment of the extracts indicated high quality products, demonstrating that this method is highly efficient and operable in DNA, RNA and hemoglobin extraction from the same blood sample of limited volume.
Li
Xi-Ping
XP
Department of Pediatrics, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Qian
Xin-Hua
XH
Liu
Zhi-Jie
ZJ
D004247:DNA / Q000302:isolation & purification*; D004586:Electrophoresis; D019075:Electrophoresis, Capillary; D006454:Hemoglobins / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D012313:RNA / Q000302:isolation & purification*
D006454:Hemoglobins; D012313:RNA; D004247:DNA