title: Human antibody response to Toscana virus glycoproteins expressed by recombinant baculovirus.
abstract: The arthropod-borne Toscana virus has been associated with acute neurological disease in humans. In this study, the viral envelope glycoproteins were expressed in soluble form in a baculovirus system. The recombinant sGN and sGC proteins were used as viral antigens in a Western blot assay to analyze the specific immune response in sera from patients with recognized virus-associated aseptic meningitis. The anti-glycoprotein and the anti-nucleoprotein N IgG responses were compared by an immunoassay based on the recombinant proteins. In this system, all the sera showed a high reactivity to the N protein, but they differed in the response to the glycoproteins.
authors.affiliation: Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Di Bonito
authors.forename: Simona
authors.initials: S
authors.lastname: Bosco
authors.forename: Stefania
authors.initials: S
authors.lastname: Mochi
authors.forename: Luisa
authors.initials: L
authors.lastname: Accardi
authors.forename: Maria G
authors.initials: MG
authors.lastname: Ciufolini
authors.forename: Loredana
authors.initials: L
authors.lastname: Nicoletti
authors.forename: Colomba
authors.initials: C
authors.lastname: Giorgi
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D016367:Baculoviridae / Q000235:genetics; D015153:Blotting, Western; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000096:biosynthesis / Q000276:immunology; D010217:Phlebotomus Fever / Q000175:diagnosis / Q000276:immunology*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D029301:Sandfly fever Naples virus / Q000235:genetics / Q000276:immunology*; D018411:Spodoptera; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007106:Immune Sera; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins