title: Trichomonas vaginalis: observation of coexistence of multiple viruses in the same isolate.
abstract: Trichomonas vaginalis is a flagellated, parasitic protozoan that inhabits the urogenital tract of humans. Some isolates of T. vaginalis are infected with a double-stranded RNA (dsRNA) virus, which was described in the literature as homogeneous icosahedral viral particles with an isometric symmetry and 33 nm in diameter. This study examined in detail the viral particles in T. vaginalis isolate 347 and describes a heterogeneous population of viral particles. The different dsRNA viruses were only observed after a change in the technique. The sample was prepared by the negative staining carbon-film method directly onto freshly cleft mica. The detected viruses ranged in size from 33 to 200 nm. Among the shapes observed were filamentous, cylindrical, and spherical particles. These results show that T. vaginalis may be a reservoir for several different dsRNA viruses simultaneously.
authors.affiliation: Universidade Santa Ursula, Rua Jornalista Orlando Dantas, 59, Botafogo RJ, CEP 22231-010, Brazil. marleneben@uol.com.br
authors.forename: Marlene
authors.initials: M
authors.lastname: Benchimol
authors.forename: T-H
authors.initials: TH
authors.lastname: Chang
authors.forename: John F
authors.initials: JF
authors.lastname: Alderete
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient / Q000379:methods; D008854:Microscopy, Electron; D016624:Negative Staining / Q000379:methods*; D012328:RNA Viruses / Q000648:ultrastructure*; D012330:RNA, Double-Stranded / Q000648:ultrastructure; D014246:Trichomonas vaginalis / Q000254:growth & development / Q000821:virology*; D014771:Virion / Q000648:ultrastructure
chemical_list: D012330:RNA, Double-Stranded