title: [Dendritic cells transfected with carcinoembryonic antigen-vaccinia recombinant virus induces CEA-specific immunity mediated by cytotoxic T lymphocytes in vitro].
abstract: OBJECTIVE
  To observe whether dendritic cells (DCs) transfected with carcinoembryonic antigen (CEA)-vaccinia recombinant virus (rV-CEA) induces cytotoxic T lymphocyte-mediated CEA-specific immunity in vitro.
  
  
  METHODS
  DCs derived from peripheral blood monocytes were transfected with rV-CEA and then cocultured with autologous T cells. The proliferation of the induced T cells and their cytotoxic activity against CEA-secreting tumor cells were assessed and compared with those of T cells induced by DCs without rV-CEA transfection.
  
  
  RESULTS
  T cells induced by DCs transfected with rV-CEA showed specific cytotoxicity against CEA-secreting tumor cells.
  
  
  CONCLUSIONS
  DCs transfected with rV-CEA can elicit activation of CEA-specific T cells.
authors.affiliation: Department of Oncology, General Hospital of Guangzhou Command, Guangzhou 510010, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Xiao-Huai
authors.initials: XH
authors.lastname: Wang
authors.forename: Tai-Cheng
authors.initials: TC
authors.lastname: Yang
authors.forename: Jiang
authors.initials: J
authors.lastname: Xian
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000276:immunology*; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D002272:Carcinoembryonic Antigen; D011993:Recombinant Fusion Proteins