Catalytic spectrofluorimetric determination of superoxide anion radical and superoxide dismutase activity using N,N-dimethylaniline as the substrate for horseradish peroxidase (HRP).
The coupled reaction of N,N-dimethylaniline (DMA) with 4-aminoantipyrine (4-AAP) using superoxide anion radical (O2-) as oxidizing agent under the catalysis of horseradish peroxidase (HRP) was studied. Based on the reaction, O2- produced by irradiating Vitamin B2, (VB2) was spectrophotometricly determined at 554 nm. The linear range of this method was 1.8 x 10(-6)-1.2 x 10(-4) mol l(-1) with a detection limit of 5.3 x 10(-7) mol l(-1). The effect of interferences on the determination of O2- was investigated. The proposed method was successfully applied to the determination of superoxide dismutase (SOD) activity in human blood and mouse blood.
Tang
Bo
B
Department of Chemistry, Shandong Normal University, Jinan, People's Republic of China. toplet@jn-public.sd.cninfo.net
Wang
Yan
Y
Chen
Zhen-zhen
ZZ
D000328:Adult; D000367:Age Factors; D000675:Ampyrone / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D051379:Mice; D008875:Middle Aged; D012256:Riboflavin / Q000528:radiation effects; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013482:Superoxide Dismutase / Q000097:blood*; D013481:Superoxides / Q000097:blood* / Q000378:metabolism
D000814:Aniline Compounds; D000675:Ampyrone; D013481:Superoxides; C015157:N,N-dimethylaniline; D006735:Horseradish Peroxidase; D013482:Superoxide Dismutase; D012256:Riboflavin