Transcriptional regulatory networks in Saccharomyces cerevisiae.
We have determined how most of the transcriptional regulators encoded in the eukaryote Saccharomyces cerevisiae associate with genes across the genome in living cells. Just as maps of metabolic networks describe the potential pathways that may be used by a cell to accomplish metabolic processes, this network of regulator-gene interactions describes potential pathways yeast cells can use to regulate global gene expression programs. We use this information to identify network motifs, the simplest units of network architecture, and demonstrate that an automated process can use motifs to assemble a transcriptional regulatory network structure. Our results reveal that eukaryotic cellular functions are highly connected through networks of transcriptional regulators that regulate other transcriptional regulators.
Lee
Tong Ihn
TI
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.
Rinaldi
Nicola J
NJ
Robert
François
F
Odom
Duncan T
DT
Bar-Joseph
Ziv
Z
Gerber
Georg K
GK
Hannett
Nancy M
NM
Harbison
Christopher T
CT
Thompson
Craig M
CM
Simon
Itamar
I
Zeitlinger
Julia
J
Jennings
Ezra G
EG
Murray
Heather L
HL
Gordon
D Benjamin
DB
Ren
Bing
B
Wyrick
John J
JJ
Tagne
Jean-Bosco
JB
Volkert
Thomas L
TL
Fraenkel
Ernest
E
Gifford
David K
DK
Young
Richard A
RA
D000465:Algorithms; D002453:Cell Cycle; D019295:Computational Biology; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D025461:Feedback, Physiological; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D016681:Genome, Fungal; D008957:Models, Genetic; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors