title: [Construction of the recombinant plasmid FasAD-pTYB2 capable of Fas activation domain fusion expression].
abstract: OBJECTIVE
  To construct the recombinant plasmid FasAD-pTYB2 for the fusion expression of Fas activation domain (FasAD).
  
  
  METHODS
  FasAD cDNA was cloned by semi-nested reverse transcriptase-polymerase chain reaction (RT-PCR), and then inserted into the universal vector of pGEM-T for the identification of its DNA sequence. The target DNA fragment was inserted into the prokaryote vector pTYB2 that expressed intein to construct the plasmid recombinant FasAD-pTYB2 which was induced by isopropylthio-beta-D-galactoside for the expression of the fusion protein.
  
  
  RESULTS
  DNA sequence analysis confirmed sequence of FasAD cDNA previously cloned, and expression of the fusion protein by the recombinant plasmid was achieved, the product recognizable by rabbit anti-human Fas polyclonal antibody as demonstrated by Western blotting analysis.
  
  
  CONCLUSIONS
  The recombinant plasmid FasAD-pTYB2 constructed in this study is capable of expressing the fusion protein of FasAD and intein, which may be significant for further study of the epitope and function of Fas antigen.
authors.affiliation: Department of Pediatrics, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Feng
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Wang
authors.forename: Yun-Shan
authors.initials: YS
authors.lastname: Ning
authors.forename: Shu-Yun
authors.initials: SY
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D010957:Plasmids / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D019014:fas Receptor / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D019014:fas Receptor