Mechanisms of monocrotophos resistance in cotton bollworm, Helicoverpa armigera (Hübner).
Insensitive acetylcholinesterase was identified as a resistance mechanism by comparing biochemical analysis with a laboratory selected monocrotophos resistant cotton bollworm (RR: 200) and the susceptible strain. The cDNA encoding AChE was cloned by the method of RACE (rapid amplification of cDNA ends). The complete AChE gene deduced from the cDNA consisted of a putative signal peptide of 32 amino acid residues, a mature protein of 615 residues, 5' untranslated regions (UTR) of 315 bp and 3' UTR of 324 bp. The coding sequence had a high degree of homology to the AChE from other insect species reported in the GenBank. After comparing analysis of the entire AChE gene sequence from 5 resistant and 6 susceptible cotton bollworm individuals, nine mutations were identified. One of them, the Ala/Thr mutation, is likely to be responsible for the AChE insensitivity to monocrotophos.
Ren
Xiaoxia
X
Key Lab of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, People's Republic of China.
Han
Zhaojun
Z
Wang
Yinchang
Y
D000110:Acetylcholinesterase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002800:Cholinesterase Inhibitors; D018076:DNA, Complementary; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides; D008969:Molecular Sequence Data; D008999:Monocrotophos; D009036:Moths / Q000201:enzymology* / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D002800:Cholinesterase Inhibitors; D018076:DNA, Complementary; D007306:Insecticides; D008999:Monocrotophos; D000110:Acetylcholinesterase