Determination of histamine by capillary zone electrophoresis with amperometric detection.
Capillary zone electrophoresis was employed for the determination of histamine using end-column amperometric detection with a carbon fiber microelectrode, at a constant potential. The optimum conditions of separation and detection were 10 mmol/L phosphate buffer, pH 5.6 for the buffer solution, 15 kV for the separation voltage, and 1.35 V (versus SCE) for the detection potential. The linear range was from 6.3 x 10(-7) to 1.5 x 1(-5) mol/L with the regression coefficient of 0.9997, and the detection limit was 4.0 x 10(-7) mol/L (S/N = 3). The proposed method was successfully applied to the direct determination of histamine in the beer samples without any sample clean-up procedures.
Zhang
Liyao
L
Department of Chemistry, Wuhan University, China.
Huang
Weihua
W
Wang
Zongli
Z
Cheng
Jieke
J
D001515:Beer / Q000032:analysis; D002021:Buffers; D004563:Electrochemistry; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006632:Histamine / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D012680:Sensitivity and Specificity
D002021:Buffers; D006632:Histamine