The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.
Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semi-conservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids.
Przetakiewicz
Jarosław
J
Plant Breeding and Acclimatization Institute, Radzików, 05-870 Błonie, Poland.
Nadolska-Orczyk
Anna
A
Orczyk
Wacław
W
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004171:Diploidy; D005819:Genetic Markers; D006824:Hybridization, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D011198:Solanum tuberosum / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers