Influence of conjugated vs. conventional linoleic acid on liver metastasis and hepatic lipidperoxidation in BOP-induced pancreatic cancer in Syrian hamster.
While conjugated linoleic acid (CLA) is regarded as an essential fatty acid with anticarcinogenic effects, conventional linoleic acid (LA) is reported to promote tumour growth in various experimental studies probably caused by high sensitivity to non-enzymatic lipid peroxidation. In order to evaluate the impact of dietary LA and CLA on liver metastasis and lipidperoxidation (LPO), 60 Syrian hamsters were injected with 10 mg N -nitrosobis-2-oxopropylamine (BOP)/kg body weight s.c. for 12 weeks. Animals were fed a special diet containing LA or CLA. The experiment was terminated after 24 weeks. Incidence, number and size of liver metastases were histologically determined. Furthermore, the activities of antioxidative enzymes and concentration of hepatic lipidperoxidation were measured intra- and extrametastatically. Incidence, number and size of liver metastases did not differ between the tumour groups. Otherwise, antioxidative enzyme activity of GSH-Px was higher in non-metastatic liver, while SOD activity and lipidperoxidation were increased in liver metastases. Conclusively there was no difference between the groups fed with LA and CLA according to the impact on liver metastasis in ductal pancreatic cancer.
Kilian
M
M
Department of General, Visceral, Vascular and Thoracic Surgery, Charité Campus Mitte, Humboldt-University of Berlin, Berlin, Germany.
Mautsch
I
I
Gregor
J I
JI
Stahlknecht
P
P
Jacobi
C A
CA
Schimke
I
I
Guski
H
H
Wenger
F A
FA
D000818:Animals; D006224:Cricetinae; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D008041:Linoleic Acids / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000139:chemically induced / Q000201:enzymology / Q000556:secondary*; D009602:Nitrosamines; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000473:pathology*; D013482:Superoxide Dismutase / Q000378:metabolism
D004042:Dietary Fats, Unsaturated; D008041:Linoleic Acids; D009602:Nitrosamines; C013622:nitrosobis(2-oxopropyl)amine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase