title: Biological variability in clinical isolates of Trichomonas vaginalis.
abstract: Eighteen clinical isolates of Trichomonas vaginalis were obtained from women who attended health centers of the Government of Madrid. A total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid Diamond medium. Pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. As could be expected, a broad variability was obtained, being some of the isolates more virulent than the reference strain. Regarding to metronidazole susceptibility, none resistant isolate was found but different degrees of susceptibility were determined.
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, Madrid, España. agbarrio@farm.ucm.es
authors.forename: Alicia
authors.initials: A
authors.lastname: Gómez-Barrio
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Nogal-Ruiz
authors.forename: David
authors.initials: D
authors.lastname: Montero-Pereira
authors.forename: Ester
authors.initials: E
authors.lastname: Rodríguez-Gallego
authors.forename: Esperanza
authors.initials: E
authors.lastname: Romero-Fernandez
authors.forename: José A
authors.initials: JA
authors.lastname: Escario
mesh_terms: D000818:Animals; D000994:Antitrichomonal Agents / Q000494:pharmacology; D005260:Female; D006801:Humans; D008795:Metronidazole / Q000494:pharmacology; D051379:Mice; D021261:Parasitic Sensitivity Tests; D014245:Trichomonas Infections / Q000188:drug therapy / Q000469:parasitology*; D014246:Trichomonas vaginalis / Q000187:drug effects / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D000994:Antitrichomonal Agents; D008795:Metronidazole