Direct visualization by confocal fluorescent microscopy of the permeation of myristoylated peptides through the cell membrane.
To study the permeability through the cellular membrane of synthetic peptides containing an hydrophobic moiety, we used a 13-mer myristoylated peptide labeled with a N-terminal fluorescent probe. After 2 h of incubation, the subcellular distribution was analyzed in intact chromaffin cells by confocal fluorescent microscopy. Our results demonstrate that myristoylated peptides diffuse into intact cells, showing an heterogeneous distribution, but they do not reach the cellular nucleous, at least during the time range used.
Enseñat-Waser
Roberto
R
Instituto de Bioingenieria, Universidad Miguel Hernandez, Alicante, Spain.
Martin
Franz
F
Barahona
Fernando
F
Vazquez
Jesús
J
Soria
Bernat
B
Reig
Juan A
JA
D000215:Acylation; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D019439:Chromaffin Cells / Q000378:metabolism* / Q000648:ultrastructure; D004058:Diffusion; D005456:Fluorescent Dyes / Q000032:analysis; D057927:Hydrophobic and Hydrophilic Interactions; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009226:Myristates / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D013347:Subcellular Fractions / Q000737:chemistry
D005456:Fluorescent Dyes; D009226:Myristates; D010455:Peptides