Effect of hyperthermic preconditioning on cold preserved rat portal veins.
OBJECTIVE
Little information is available regarding the effect of cold storage and hyperthermic preconditioning on the contractile responses of hepatic vessels. We then studied, after cold preservation, the in vitro contractile responses of rat portal veins (RPV) isolated from normal rats or from rats previously subjected to hyperthermia.


METHODS
Rats were or were not subjected to hyperthermia 24 h before the RPV isolation. Then, RPV were stored at 4 degrees C in Krebs-Henseleit bicarbonate (KHB) or University of Wisconsin solution or conserved at 20 degrees C in KHB solution. Control RPV were tested after rat sacrifice.


RESULTS
The contractile responses were importantly decreased in RPV conserved at room temperature. The morphology of the vessels was altered and the heat shock protein 70 (Hsp70) protein expression disappeared. These abnormalities were prevented by cold preservation. The type of preservation solution did not interfere with the beneficial effect. Hyperthermic preconditioning increased the expression of Hsp70 protein in freshly isolated and cold preserved RPV but decreased the contractile responses. In RPV conserved at room temperature, hyperthermic preconditioning further worsened the decreased contractile response.


CONCLUSIONS
Thus, hyperthermic preconditioning, which is beneficial in protecting hepatic injury following cold preservation, alters the contractile responses of RPV.
Pastor
Catherine M
CM
Division d'Hépatologie et de Gastro-entérologie, Hôpital Cantonal Universitaire de Genève, Rue Micheli-du-Crest, 24, 1211 Geneva 14, Switzerland. catherine.pastor@hcuge.ch
Frossard
Jean-Louis
JL
Mentha
Gilles
G
Mastrangelo
Dominique
D
Quadri
Rafael
R
Hadengue
Antoine
A
D000818:Animals; D018834:Chaperonin 60 / Q000096:biosynthesis; D003080:Cold Temperature; D006085:Graft Survival; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006979:Hyperthermia, Induced; D019194:Ischemic Preconditioning; D008297:Male; D019188:Organ Preservation Solutions; D011169:Portal Vein / Q000378:metabolism / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature; D014661:Vasoconstriction / Q000187:drug effects
D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D019188:Organ Preservation Solutions