Generation of multicolored, prestained molecular weight markers for gel electrophoresis.
Molecular weight marker proteins are routinely used in sodium dodecyl sulfate-polyacrylamide gel electrophoresis to estimate the relative molecular mass of specific proteins within a sample. This report describes a simple procedure for the generation of multicolored molecular weight proteins using a variety of Remazol-reactive textile dyes. These multicolored proteins provide a set of unambiguous markers for gel electrophoresis. Furthermore, the colored markers can be used in conjunction with Western blotting techniques to provide a visual display of marker proteins on the transfer membrane.
Compton
Mark M
MM
Department of Poultry Science, The University of Georgia, Athens, GA 30602-2772, USA. mompton@arches.uga.edu
Lapp
Stacey A
SA
Pedemonte
Ron
R
D003116:Color; D004396:Coloring Agents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008970:Molecular Weight; D012015:Reference Standards
D004396:Coloring Agents