title: Changes in morphology and inward rectifier currents in human atrial myocytes depend on culture conditions.
abstract: Human atrial myocytes were cultured under systematically varied conditions in order to obtain stable cells for future gene manipulation. Transient (I(to)) and sustained outward current (I(so)), and voltage- and muscarinic receptor-activated inward rectifier K(+) currents (I(K1), I(K,ACh)) were measured in freshly isolated cells and after 5 days in culture. Myocytes were grown on polylysin or laminin in medium with or without 10 % serum (medium+S, medium-S). Cultured myocytes dedifferentiated to a greater extent in medium+S than medium-S, but independent of the chemical nature of the adherence surface. Apparent surface area increased in medium+S, whereas membrane capacitance declined under all culture conditions. I(to) of myocytes cultured in medium-S was increased. Myocytes grown on polylysin and laminin exhibited reduced I(K1) current density. Under all culture conditions, I(K,ACh) was attenuated with carbachol but hardly affected with sphingosine-1-phosphate as agonists. In conclusion, morphological and electrophysiological changes depended on serum in the culture medium rather than on adherence surface being coated with laminin or polylysin.
authors.affiliation: Bayer AG, Abt Sicherheitsstudien, Wuppertal, Germany.
authors.forename: Herbert M
authors.initials: HM
authors.lastname: Himmel
authors.forename: Martina
authors.initials: M
authors.lastname: Pietsch
authors.forename: Uwe
authors.initials: U
authors.lastname: Streller
authors.forename: Eva M
authors.initials: EM
authors.lastname: Graf
authors.forename: Ursula
authors.initials: U
authors.lastname: Ravens
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D001464:Barium / Q000494:pharmacology; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004351:Drug Resistance; D025802:Electric Capacitance; D004553:Electric Conductivity; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006325:Heart Atria; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying / Q000187:drug effects / Q000502:physiology*; D011726:Pyridinium Compounds; D013194:Staining and Labeling
chemical_list: C089669:1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine; D016895:Culture Media, Serum-Free; D005456:Fluorescent Dyes; D024661:Potassium Channels, Inwardly Rectifying; D011726:Pyridinium Compounds; D001464:Barium; D000109:Acetylcholine