title: Solid-state fermentation for production of phytase by Rhizopus oligosporus.
abstract: Solid-state fermentation of coconut oil cake has been carried out with Rhizopus oligosporus for the production of phytase. Phytase is used commercially in the animal feed industry to improve animal performance because there is a substantial and growing interest among swine and poultry producers in the application of phytase to improve the nutritional quality in animal feeds. Demonstrated benefits include improved feed yield ratios and reduction in the environmental costs associated with the disposal of animal wastes. We report the production of extracellular phytase by R. oligosporus under solid-state fermentation using coconut oil cake as substrate. Maximal enzyme production (14.29 U/g of dry substrate) occurred at pH 5.3, 30 degrees C, and 54.5% moisture content after 96 h of incubation. The addition of extra nutrients to the substrate resulted in inhibition of product formation. The results indicate the scope for production of phytase using coconut oil cake as solid substrate without additional nutrients.
authors.affiliation: Biotechnology Division, Regional Research Laboratory (CSIR), Trivandrum, India.
authors.forename: Abdulhameed
authors.initials: A
authors.lastname: Sabu
authors.forename: Saradamma
authors.initials: S
authors.lastname: Sarita
authors.forename: Ashok
authors.initials: A
authors.lastname: Pandey
authors.forename: Barbara
authors.initials: B
authors.lastname: Bogar
authors.forename: George
authors.initials: G
authors.lastname: Szakacs
authors.forename: Carlos Ricardo
authors.initials: CR
authors.lastname: Soccol
mesh_terms: D010832:6-Phytase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D000074263:Coconut Oil; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D010833:Phytic Acid / Q000494:pharmacology; D010938:Plant Oils / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology*; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000032:analysis
chemical_list: D003470:Culture Media; D010938:Plant Oils; D014867:Water; D010833:Phytic Acid; D010832:6-Phytase; D000074263:Coconut Oil