title: [Relationship between the different replication status of HBV and mutations in core promoter in mother and children infected by mother-to-infant transmission].
abstract: OBJECTIVE
  To understand the relationship between the different replication status of HBV and mutations in core promoter in mother and child infected by mother-to-infant transmission.
  
  
  METHODS
  Core promoter was amplified by PCR and cloned into pGEM-T vector with T-A cloning technique. The recombinant plasmid pGEM-PreS/S was confirmed by digestion with restriction enzyme Apa I and Sac I. Two clones were selected to sequence each patient.
  
  
  RESULTS
  Every pair of mother and child had same serotype and genotype and the homology of nucleotides encoding "a" determinant was 98 to 100%. The number of mutations in core promoter in patients with high replication status was more than that of low replication status. Mutations were distributed in BCP and Kunitz-type serine protease inhibitor region mainly. This difference was not associated with mother or child.
  
  
  CONCLUSIONS
  The different replication status of HBV is caused by mutations in core promoter in mother and child infected by mother-to-infant transmission and seems not to be associated with the development status.
authors.affiliation: Research Institute of Viral Hepatitis, Chongqing 400010, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
authors.forename: Yuling
authors.initials: Y
authors.lastname: Qing
authors.forename: Mingli
authors.initials: M
authors.lastname: Peng
authors.forename: Ning
authors.initials: N
authors.lastname: Ling
mesh_terms: D002648:Child; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis* / Q000235:genetics*; D005838:Genotype; D006509:Hepatitis B / Q000635:transmission / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D007223:Infant; D018445:Infectious Disease Transmission, Vertical; D009035:Mothers; D009154:Mutation; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic
chemical_list: D004279:DNA, Viral