Effect of psoralens on Fenton-like reaction generating reactive oxygen species.
Psoralens (psoralen, 5-methoxypsoralen, 8-methoxypsoralen, khellin, and visnagin) in 1 mM doses were shown to enhance the generation of reactive oxygen species, such as the hydroxyl radical (HO*), the superoxide anion radical (O2(-)), and singlet oxygen ((1)O(2)), from the system generating chemiluminescence (CL), as well as free radicals in the absence of light. The system that generated CL was made up of CoCl(2) and H(2)O(2). Incubation of psoralens in 0.2 mM doses with the generating system showed that only 8-methoxypsoralen and khellin have antioxidative effects. Antioxidative effects were also observed in the case of visnagin but in low concentration (0.05 mM). High doses of psoralens (1 mM) showed prooxidative effects. Measurements were done using a deoxyribose assay, the CL method, and spin-trapping with 5,5-dimethyl-1-pyrroline-N-oxide and 2,2,6,6-tetramethylpiperidine combined with electron spin resonance spectroscopy and spectrophotometry methods.
Aboul-Enein
Hassan Y
HY
Pharmaceutical Analysis Laboratory, Biological & Medical Research Department (MBC-03-65), King Faisal Specialist Hospital & Research Centre, P.O. Box 3354, Riyadh 11211, Saudi Arabia. enein@kfshrc.edu.sa
Kladna
Alexandra
A
Kruk
Irena
I
Lichszteld
Krzysztof
K
Michalska
Teresa
T
D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000138:chemical synthesis / Q000737:chemistry; D011564:Furocoumarins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D007700:Kinetics; D008163:Luminescent Measurements; D015394:Molecular Structure; D009589:Nitrogen Oxides / Q000138:chemical synthesis / Q000737:chemistry; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000138:chemical synthesis* / Q000737:chemistry; D013997:Time Factors
C045076:Fenton's reagent; D005609:Free Radicals; D011564:Furocoumarins; D009589:Nitrogen Oxides; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D007501:Iron; C039900:nitroxyl