title: Preparation and identification of monoclonal antibodies against human brain-derived neurotrophic factor.
abstract: OBJECTIVE
  To prepare monoclonal antibodies (mAbs) against human brain-derived neurotrophic factor (BDNF).
  
  
  METHODS
  BALB/C mice were immunized with purified BDNF protein in conjunction with acid-treated Salmonella (antigen:thallus=1:5), and mAbs were subsequently derived by hybridoma technique.
  
  
  RESULTS
  Three hybridoma cell lines secreting anti-BDNF mAbs were obtained, designated as B1, B2 and 4D1 respectively and all categorized into IgG1 subtype. The titers of the mAbs in the ascitic fluid of the rats ranged from 1x10(6) to 1x10(5), and their relative affinities were B2>B1>4D1.
  
  
  CONCLUSIONS
  mAbs against BDNF are successfully prepared, which can be instrumental for further study of the expression and distribution of BDNF in vivo, and the detection of BDNF produced by genetic engineering.
authors.affiliation: Department of Respiratory Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Su
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Zhang
authors.forename: Chang-Xuan
authors.initials: CX
authors.lastname: You
authors.forename: Jin-Ya
authors.initials: JY
authors.lastname: Wu
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Che
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001921:Brain; D019208:Brain-Derived Neurotrophic Factor / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D019208:Brain-Derived Neurotrophic Factor