Advantages of soxflo extractions for phytochemical analysis and bioassay screening. 1. Terpenoids.
The Soxflo technique was evaluated for the rapid extraction of plant materials (<90 min) at room temperature. Qualitatively similar chromatograms were obtained by gas chromatography and thin layer chromatography (TLC) with Soxflo (SoF) and Soxhlet (SoL) extracts. Sequential solvent extractions by SoF gave slightly higher yields (132%) of five major sesquiterpenoids. TLC revealed that SoF extractions at room temperature were more selective as extracts contained compounds with a narrower range of R(f)() values. This means that the SoF technique offers the potential for one-step extractions and partial fractionation. This study also showed that there were large differences in the volatile composition of dried and fresh Piper fruits: dried fruits had predominantly sesquiterpenoids while fresh fruits had considerable quantities of both mono- and sesquiterpenoids. This is the first report of alpha-guaiene and alpha- and beta-selinene in Piper guineense fruits. It is suggested that the SoF technique can be useful for the screening of large numbers of plants for phytochemicals or for the preparation of plant extracts for subsequent bioassay studies.
Ntonifor
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Department of Agriculture, The University of Reading, P.O. Box 236, Reading RG6 6AT, United Kingdom.
Brown
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Mueller-Harvey
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D002855:Chromatography, Thin Layer / Q000379:methods; D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D029582:Meliaceae / Q000737:chemistry*; D009822:Oils, Volatile / Q000032:analysis; D031701:Piper / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis*; D012997:Solvents; D013729:Terpenes / Q000032:analysis / Q000302:isolation & purification*
D009822:Oils, Volatile; D010936:Plant Extracts; D012997:Solvents; D013729:Terpenes