Lactate dehydrogenase estimation in follicular fluid: correlation with patient age, follicle size and super ovulation in ART cycles.
OBJECTIVE
To quantify the lactate dehydrogenase (LDH) level in human follicular fluids, and to define its relationship with follicle size, patient age, serum estradiol (E(2)) level, and the amount of gonadotropins administered during superovulation in ART cycles.


METHODS
In this prospective study, 21 women undergoing ART treatment were selected. Follicular fluid from the largest follicle of both ovaries was collected from each patient on the day of oocyte aspiration and analyzed for LDH. Serum oestradiol was estimated on the day of hCG administration. Relationship between LDH level and (1) patient age, (2) follicle size, (3) follicle stimulating hormone administered during superovulation period and (4) serum oestradiol was studied.


RESULTS
LDH activity increased with chronological age of the patient. As follicular size (diameter) increased, increase in the LDH concentration in follicular fluid was observed. Serum estradiol level did not show any relationship with LDH activity. Similarly, administration of various doses of follicle stimulating hormone during superovulation did not show any correlation with LDH level.


CONCLUSIONS
Follicular fluid LDH level has shown association with patient age and the follicle size, however, no significant association was found with other parameters studied.
Adiga
Satish Kumar
SK
Manipal Assisted Reproduction Centre, Kasturba Medical College, Manipal 576 119, India. satish.adiga@kmc.manipal.edu
Kumar
Pratap
P
Bijoor
Shivananda Nayak
SN
D000328:Adult; D000375:Aging; D004624:Embryo Transfer; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D015571:Follicular Fluid / Q000201:enzymology*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D006080:Ovarian Follicle / Q000033:anatomy & histology*; D011247:Pregnancy; D011446:Prospective Studies; D027724:Reproductive Techniques, Assisted; D013480:Superovulation
D004958:Estradiol; D005640:Follicle Stimulating Hormone; D007770:L-Lactate Dehydrogenase