title: Screening of tumor necrosis factor-alpha-binding peptides by phage display peptide library.
abstract: OBJECTIVE
  To identify and characterize tumor necrosis factor (TNF)-alpha-binding peptides from c7c phage display peptide library, in an attempt to find short peptides that can be used as antagonist of TNF-alpha.
  
  
  METHODS
  The TNF-alpha-binding peptides were screened from c7c phage display peptide library by using rhTNF-alpha as target protein and identified by sandwich ELISA.
  
  
  RESULTS
  After 3 rounds of screening, 11 of 23 phage clones were identified as positive clones which can bind to rhTNF-alpha. The amino acid sequence in two of these 11 clones is c-ALWHWWH-c, and that in the others is c-(T/S)WLHWWA-c.
  
  
  CONCLUSIONS
  These phage display peptides are TNFalpha-binding peptides.
authors.affiliation: Department of Immunology, First Military Medical University, Guangzhou 510515, China.
authors.forename: Hai-Ping
authors.initials: HP
authors.lastname: Guo
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Luo
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: Ning
authors.initials: N
authors.lastname: Fu
mesh_terms: D006801:Humans; D007158:Immunologic Techniques; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D019151:Peptide Library; D010455:Peptides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha