Detection of mouse hepatitis virus infection by assay of anti-liver autoantibodies.
The observation that mice infected with mouse hepatitis virus (MHV) develop autoantibodies directed mainly to liver fumarylacetoacetate hydrolase (FAH) enabled the development of an ELISA applicable to the detection of MHV-infection. The method, based on the titration of antibodies to semipurified FAH from rat liver, is easy, economical, and does not require the isolation of viral proteins from large MHV stocks. Furthermore, since sera from mice immunized with a purified fraction of the rat liver enzyme do react with its homologous protein, this antiserum can be used as a positive control avoiding the manipulation of samples from MHV-infected animals.
Mathieu
Patricia A
PA
Facultad de Farmacia y Bioquímica, Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Junín 956-1113, Buenos Aires, Argentina.
Gómez
Karina A
KA
Coutelier
Jean-Paul
JP
Retegui
Lilia A
LA
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D018352:Coronavirus Infections / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006867:Hydrolases / Q000276:immunology*; D008099:Liver / Q000201:enzymology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D006517:Murine hepatitis virus
D000914:Antibodies, Viral; D001323:Autoantibodies; D006867:Hydrolases; C020156:fumarylacetoacetase