title: An active compound against allergen absorption in hypoallergenic wheat flour produced by enzymatic modification.
abstract: Hypoallergenic wheat flour produced by modification with cellulase and actinase showed inhibitory activity against ovalbumin permeation in an in vitro model by using the Caco-2 cell monolayer. The activity was found in the cellulase preparation used for producing the flour. An active compound was isolated by HPLC and identified as Trp-Ser-Asn-Ser-Gly-Asn-Phe-Val-Gly-Gly-Lys by 1H-NMR data and Edman degradation. The undecapeptide, some oligopeptides with the N-terminal sequences and Trp ethyl ester showed activity at 10(-7) M, acetyl Trp being active at 10(-2) M. These data suggest that the Trp residue without a free carboxyl group would be required for the inhibitory activity of ovalbumin absorption through the intestinal tract.
authors.affiliation: Department of Nutrition and Life-science, Takasaki University of Health and Welfare, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Tesaki
authors.forename: Jun
authors.initials: J
authors.lastname: Watanabe
authors.forename: Soichi
authors.initials: S
authors.lastname: Tanabe
authors.forename: Kei
authors.initials: K
authors.lastname: Sonoyama
authors.forename: Eri
authors.initials: E
authors.lastname: Fukushi
authors.forename: Jun
authors.initials: J
authors.lastname: Kawabata
authors.forename: Michiko
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000042:Absorption; D000485:Allergens / Q000378:metabolism*; D000595:Amino Acid Sequence; D018938:Caco-2 Cells; D002480:Cellulase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases / Q000378:metabolism*; D005433:Flour / Q000032:analysis*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010047:Ovalbumin / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D014908:Triticum / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism
chemical_list: D000485:Allergens; D010446:Peptide Fragments; D010047:Ovalbumin; D002480:Cellulase; D010450:Endopeptidases