title: Response regulator important in pathogenesis of Streptococcus suis serotype 2.
abstract: In this study, we describe the first response regulator of Streptococcus suis serotype 2, designated RevS. RevS was cloned and the sequence was determined. No histidine kinase was found in the vicinity ofrevS, therefore RevS was considered to be an orphan response regulator. An isogenic knock-out mutant of RevS was shown to be attenuated in colonising the S. suis specific organs in a competitive assay in four piglets, indicating thatrevS plays a role in the pathogenesis of S. suis infections. We analysed the protein expression profiles of various fractions of the wild-type strain 10 and the mutant strain 10DeltaRevS. The expression of known virulence factors ofS. suis by wild-type and the mutant strain, was not different. However, one protein in the protoplast fraction, with unknown identity was shown to be repressed by revS in the exponential growth phase of the mutant.
authors.affiliation: Department of Medical Microbiology, University of Amsterdam, Academical Medical Center, The Netherlands. a.degreeff@id.wag-ur.nl
authors.forename: Astrid
authors.initials: A
authors.lastname: de Greeff
authors.forename: Herma
authors.initials: H
authors.lastname: Buys
authors.forename: Loek
authors.initials: L
authors.lastname: van Alphen
authors.forename: Hilde E
authors.initials: HE
authors.lastname: Smith
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis; D001483:Base Sequence; D003001:Cloning, Molecular; D000071677:Histidine Kinase; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000502:physiology; D012703:Serotyping; D015398:Signal Transduction; D017011:Streptococcus suis / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D013552:Swine; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011494:Protein Kinases; D000071677:Histidine Kinase