title: Has the oxidative stress a role in late phase of experimental acute necrotizing pancreatitis?
abstract: OBJECTIVE
  Reactive oxygen species and derivatives which are implicated in pathogenesis of pancreatitis are a group of substances which may provide distribution of proenzymes following destruction of acinary cells and may activate lysosomal enzymes. We aimed to show that oxidative stress in rats rendered acute necrotizing pancreatitis experimentally.
  
  
  METHODS
  Twenty Spraque-Dawley rats were included into the study and they were divided into the subgroups of pancreatitis and controls. Taurocholate (3%) in doses of 1 mL/kg was administered into the biliopancreatic duct of the rats to render them with acute necrotizing pancreatitis. Rats were killed at 48 hours after the procedure. Malondealdehyde levels and superoxide dismutase activities in pancreatic tissue and erythrocytes were determined.
  
  
  RESULTS
  Significantly elevated tissue and erythrocyte malondealdehyde levels were found in rats with pancreatitis (p < 0.001). Superoxide dismutase activities in both tissues and erythrocytes of the rats with pancreatitis were significantly lower than those of the controls (p < 0.001).
  
  
  CONCLUSIONS
  High levels of malondealdehyde in tissues and erythrocytes reveal the importance of reactive oxygen species in this process. To reveal the reason of decreased activities of superoxide dismutase in tissues and erythrocytes of the rats with pancreatitis may lead to a better understanding of pathogenesis of the disease and appearance of new treatment modalities.
authors.affiliation: Gülhane School of Medicine, Department of Surgery, Etlik, 06018, Ankara, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yasar
authors.forename: M Refik
authors.initials: MR
authors.lastname: Mas
authors.forename: Bilgin
authors.initials: B
authors.lastname: Comert
authors.forename: Cemal
authors.initials: C
authors.lastname: Akay
authors.forename: Salih
authors.initials: S
authors.lastname: Deveci
authors.forename: M Ilker
authors.initials: MI
authors.lastname: Yilmaz
authors.forename: Bulent
authors.initials: B
authors.lastname: Kocer
authors.forename: Nuket
authors.initials: N
authors.lastname: Mas
authors.forename: Huseyin
authors.initials: H
authors.lastname: Doruk
authors.forename: I Hakki
authors.initials: IH
authors.lastname: Kocar
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D019283:Pancreatitis, Acute Necrotizing / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric; D013482:Superoxide Dismutase / Q000378:metabolism; D013656:Taurocholic Acid
chemical_list: D008315:Malondialdehyde; D013656:Taurocholic Acid; D013482:Superoxide Dismutase