[Substance P up-regulates the TGF-beta 1 mRNA expression of human dermal fibroblasts in vitro].
OBJECTIVE
To investigate the role of substance P in the formation of hypertrophic scar.


METHODS
Dermal fibroblasts derived from human normal skin were cultured with substance P alone or together with selective non-peptide NK-1 tachykinin antagonist, L-703, 606 oxalate salt. The effect of substance P on proliferation of fibroblasts was measured by MTT assay. Furthermore, the TGF-beta 1 mRNA expression in the fibroblasts was determined by in situ hybridization and image analysis.


RESULTS
Substance P enhanced fibroblast proliferation dose-dependently, which showed the maximum rate when the concentration of substance P was 25 ng/ml or higher in the culture media. By 48 hours cultured with 25 ng/ml of substance P, the fibroblasts expressed TGF-beta 1 mRNA more significantly than the fibroblasts without substance P. The effects of substance P on both fibroblast proliferation and TGF-beta 1 mRNA expression could be antagonized by L-703, 606 oxalate salt.


CONCLUSIONS
The results suggest that substance P may play an important role in phenotype changes of fibroblasts in skin scarring.
Hu
Dahai
D
Department of Burns, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Chen
Bi
B
Zhu
Xiongxinag
X
Tao
Ke
K
Tang
Chaowu
C
Wang
Jianbo
J
D002455:Cell Division; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D064729:Neurokinin-1 Receptor Antagonists; D011812:Quinuclidines / Q000494:pharmacology; D012333:RNA, Messenger; D013373:Substance P / Q000378:metabolism* / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000235:genetics*; D053773:Transforming Growth Factor beta1; D015854:Up-Regulation
D064729:Neurokinin-1 Receptor Antagonists; D011812:Quinuclidines; D012333:RNA, Messenger; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C076851:L 703606; D013373:Substance P