title: Short estrous cycles and estrous signs after premature ovulations induced with cloprostenol and gonadotropin-releasing hormone in cyclic dairy cows.
abstract: The aim of the present study was to confirm earlier findings, obtained with a small number of animals, that gonadotropin-releasing hormone (GnRH) can shorten corpus luteum functional life when it is administered 24 h after cloprostenol (PG) treatments given 7-9 days after estrus. In addition, the effects of two treatments, PG alone or PG + GnRH given before mid-diestrus, on signs of estrus were studied. Sixty cows in farm conditions were used in the experiment. Eight days after natural estrus, they were given an intramuscularly (i.m.) treatment of cloprostenol (0.5 mg). The animals were then divided into two groups. One group (n = 25) received an i.m. treatment of gonadorelin (0.1 mg) 24 h after the PG treatment (PG + GnRH group), while another group (n = 35) served as controls without any further treatment (PG group). Estrous signs were recorded. Progesterone concentrations were measured from samples of whole milk. No short cycles were observed in the PG group, whereas 33% of the cows in the PG + GnRH group exhibited premature luteal regression (P < 0.05). Cloprostenol treatment on Day 8 had no effect on the intensity of the estrous signs. Instead, GnRH treatment 24 h after PG treatment weakened the estrous signs significantly (P < 0.01). It is concluded that GnRH administration 24 h after a PG treatment given 8 days after estrus can cause short estrous cycles in some cows on an individual basis.
authors.affiliation: Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Saarentaus, Finland. juhani.taponen@helsinki.fi
authors.forename: J
authors.initials: J
authors.lastname: Taponen
authors.forename: M
authors.initials: M
authors.lastname: Kulcsár
authors.forename: T
authors.initials: T
authors.lastname: Katila
authors.forename: L
authors.initials: L
authors.lastname: Kátai
authors.forename: G
authors.initials: G
authors.lastname: Huszenicza
authors.forename: H
authors.initials: H
authors.lastname: Rodríguez-Martínez
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D003008:Cloprostenol / Q000494:pharmacology*; D003338:Corpus Luteum / Q000187:drug effects* / Q000502:physiology; D030762:Estrous Cycle / Q000187:drug effects* / Q000502:physiology; D004971:Estrus / Q000187:drug effects / Q000502:physiology; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D008892:Milk / Q000737:chemistry; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*; D011374:Progesterone / Q000378:metabolism
chemical_list: D007987:Gonadotropin-Releasing Hormone; D003008:Cloprostenol; D011374:Progesterone