Regulatory expression of MDP77 protein in the skeletal and cardiac muscles.
The mdp77 gene was first cloned from the cDNA library of denervated chick muscles, while its role(s) in vivo was unknown. In the present study, using specific polyclonal antibodies against MDP77, we show that MDP77 was expressed specifically in the skeletal and cardiac muscle, and confirm its presence in the cytoplasm of the extrafusal muscle fibers. In mature muscles, MDP77 immunoreactivity was observed in a repetitive manner along the sarcomere. The onset of MDP77 expression occurred just after myotube formation both in vivo and in vitro. Furthermore, MDP77 was enriched in the intrafusal muscle fibers. Our findings suggest that MDP77 plays an important role(s) in the differentiation, maturation and function of both the skeletal and cardiac muscles.
Fujimori
Kazuhiro E
KE
Division for Human Life Technology, National Institute of Advanced Industrial Science and Technology (AIST), Midorigaoka, Ikeda, Osaka 563-8577, Japan. k-fujimori@aist.go.jp
Uyeda
Atsuko
A
Taguchi
Takahisa
T
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins; D011233:Precipitin Tests; D015854:Up-Regulation
C406335:MDP77 protein, Gallus gallus; D009414:Nerve Growth Factors; D010940:Plant Proteins