Nogo-A expression in mature oligodendrocytes of rat spinal cord in association with specific molecules.
Nogo-A is known as an oligodendrocyte/myelin-associated molecule having an inhibitory effect on neurite outgrowth in the central nervous system. During development, starting from P21 Nogo-A was detected in the cytoplasm of mature oligodendrocytes with compact myelin sheaths in the rat spinal cord. COS7 cells transfected with recNogo-A displayed strong Nogo-A immunoreactivity in their cytoplasm as well as on the mitotic spindle. Nogo-A was not detected in membrane protein fractions from transfected plus biotinylated COS7 cells. Nogo-A was co-immunoprecipitated with alpha-tubulin and myelin basic protein (MBP) from rat brain tissue. These results show that Nogo-A is expressed in association with tubulin and MBP in the mature oligodendrocytes.
Taketomi
Masanori
M
Department of Anatomy and Neurobiology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan. taketomi@anat2.med.kyoto-u.ac.jp
Kinoshita
Nagatoki
N
Kimura
Kazushi
K
Kitada
Masaaki
M
Noda
Toru
T
Asou
Hiroaki
H
Nakamura
Takashi
T
Ide
Chizuka
C
D000818:Animals; D000831:Animals, Newborn; D019556:COS Cells; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D004676:Myelin Basic Protein / Q000096:biosynthesis* / Q000648:ultrastructure; D009185:Myelin Proteins / Q000096:biosynthesis* / Q000648:ultrastructure; D009186:Myelin Sheath / Q000378:metabolism / Q000648:ultrastructure; D000070798:Nogo Proteins; D009836:Oligodendroglia / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000378:metabolism* / Q000648:ultrastructure; D014404:Tubulin / Q000096:biosynthesis* / Q000648:ultrastructure
D004676:Myelin Basic Protein; D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603197:Rtn4 protein, rat; D014404:Tubulin