title: 5-HT1B receptor knockout mice exhibit an enhanced response to constant light.
abstract: Serotonin (5-HT) can act presynaptically at 5-HT1B receptors on retinal terminals in the suprachiasmatic nucleus (SCN) to inhibit glutamate release, thereby modulating the effects of light on circadian behavior. 5-HT1B receptor agonists (1) inhibit light-induced phase shifts of circadian activity rhythms, (2) attenuate light-induced Fos expression in the SCN, and (3) reduce the amplitude of optic nerve-evoked excitatory postsynaptic currents in SCN neurons in vitro. To determine whether functional disruption of the 5-HT1B presynaptic receptors would result in an amplified response of the SCN to light, the period (tau) of the circadian rhythm of wheel-running activity was estimated under several different conditions in 5-HT1B receptor knockout (KO) mice and genetically matched wild-type animals. Under constant light (LL) conditions, the tau of 5-HT1B receptor KO mice was significantly greater than the tau of wild-type mice. A quantitative analysis of the wheel-running activity revealed no differences between wild-type and KO mice in either total activity or the temporal distribution of activity under LL conditions, suggesting that the observed increase in tau was not a function of reduced activity. Under constant dark conditions, the period of the circadian rhythm of wheel-running activity of wild-type and 5-HT1B receptor KO mice was similar. In addition, no differences were noted between wild-type and 5-HT1B receptor KO mice in the rate of reentrainment to a 6 h phase advance in the 12:12 light:dark cycle or in phase shifts in response to a 10 min light pulse presented at circadian time 16. The enhanced response of the SCN circadian clock of the 5-HT1B receptor KO mice to LL conditions is consistent with the hypothesis that the endogenous activation of 5-HT1B presynaptic receptors modulates circadian behavior by attenuating photic input to the SCN.
authors.affiliation: Department of Anatomy and Neurobiology, Colorado State University, Fort Collins 80523-1670, USA. psollars@lamar.colostate.edu
authors.forename: Patricia J
authors.initials: PJ
authors.lastname: Sollars
authors.forename: Malcolm D
authors.initials: MD
authors.lastname: Ogilvie
authors.forename: Michael A
authors.initials: MA
authors.lastname: Rea
authors.forename: Gary E
authors.initials: GE
authors.lastname: Pickard
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D008029:Lighting; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009043:Motor Activity / Q000502:physiology; D017440:Photoperiod; D044302:Receptor, Serotonin, 5-HT1B; D011985:Receptors, Serotonin / Q000235:genetics*; D013493:Suprachiasmatic Nucleus / Q000502:physiology; D014795:Visual Pathways / Q000502:physiology
chemical_list: D044302:Receptor, Serotonin, 5-HT1B; D011985:Receptors, Serotonin