Longitudinal (1)H relaxation optimization in TROSY NMR spectroscopy.
A general method to enhance the sensitivity of the multidimensional NMR experiments performed at high-polarizing magnetic field via the significant reduction of the longitudinal proton relaxation times is described. The method is based on the use of two vast pools of "thermal bath" 1H spins residing on hydrogens covalently attached to carbon and oxygen atoms in 13C,15N labeled and fully protonated or fractionally deuterated proteins to uniformly enhance longitudinal relaxation of the 1HN spins and concomitantly the sensitivity of multipulse NMR experiments. The proposed longitudinal relaxation optimization is implemented in the 2D [15N,1H]-LTROSY, 2D [15N,1H]-LHSQC and 3D LTROSY-HNCA experiments yielding the factor 2-2.5 increase of the maximal signal-to-noise ratio per unit time at 600 MHz. At 900 MHz, the predicted decrease of the 1HN longitudinal relaxation times can be as large as one order of magnitude, making the proposed method an important tool for protein NMR at high magnetic fields.
Pervushin
Konstantin
K
Laboratorium für Physikalische Chemie, Eidgenössische Technische Hochschule Hönggerberg, CH-8092 Zürich, Switzerland. kopeko@phys.chem.ethz.ch
Vögeli
Beat
B
Eletsky
Alexander
A
D001412:Bacillus subtilis / Q000201:enzymology; D002826:Chorismate Mutase / Q000737:chemistry*; D007700:Kinetics; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011522:Protons; D025801:Ubiquitin / Q000737:chemistry*
D009587:Nitrogen Isotopes; D011522:Protons; D025801:Ubiquitin; D002826:Chorismate Mutase