Effects of gentamicin, lipopolysaccharide, and contrast media on immortalized proximal tubular cells.
Aminoglycosides are widely used in the treatment of gram-negative bacterial infections. Gentamicin (GE) acts mainly in proximal tubular cells, where it is uptake via organic anion transport system and it induces a high incidence of nephrotoxicity, which is characterized by tubular necrosis leading to acute renal failure in 10 to 50% of patients. Gram-negative bacteria have lipopolysaccharide (LPS) which is an endotoxin that causes renal damage. Moreover, many patients are undergone exams using radiologic contrast, which is a risk factor to induce a hemodynamic change in the kidney and to develop acute renal failure. Intracellular calcium [Ca2+]i is involved in renal cellular injury and maybe mediate the effects provoked by these drugs. This study was performed to evaluate necrosis, apoptosis and intracellular calcium levels ([Ca2+]i) in LLC-PK1 (epithelial cell line from pig kidney) induced by GE associated with LPS and a low-osmolality media, Hexabrix (HE).
Cunha
Maria A
MA
Nephrology Division, Esola Paulista de Medicina-UNIFESP, São Paulo, Brazil. Adelaide@nefro.com.br
Schor
Nestor
N
D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002118:Calcium / Q000032:analysis; D003287:Contrast Media / Q000009:adverse effects*; D004195:Disease Models, Animal; D005839:Gentamicins / Q000009:adverse effects*; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D007485:Ioxaglic Acid / Q000009:adverse effects*; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D018374:LLC-PK1 Cells / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D008070:Lipopolysaccharides / Q000009:adverse effects*; D013552:Swine
D000900:Anti-Bacterial Agents; D003287:Contrast Media; D005839:Gentamicins; D008070:Lipopolysaccharides; D002118:Calcium; D007485:Ioxaglic Acid