title: Developmental changes in expression of small GTPase RhoG mRNA in the rat brain.
abstract: We have recently reported that RhoG, a member of Rho family small GTPases, is involved in neurite outgrowth in cultured neuronal cells. Here, we report the expression of RhoG mRNA in the developing rat brain by in situ hybridization analysis. At embryonic day 16, RhoG expression was observed throughout the ventricular zone, but was down-regulated in the region at birth. On the other hand, RhoG expression at postnatal day 20 was highly enriched in white matter tracts, including the corpus callosum, the anterior commissure, and the cerebellar white matter, and double-labeling experiments demonstrated that major RhoG-expressing cells in white matter tracts were oligodendrocytes. These results suggest distinct pre- and postnatal roles of RhoG in the development of the central nervous system.
authors.affiliation: Laboratory of Molecular Neurobiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Hironori
authors.initials: H
authors.lastname: Katoh
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakamura
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Mori
authors.forename: Manabu
authors.initials: M
authors.lastname: Negishi
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology*; D004622:Embryo, Mammalian / Q000033:anatomy & histology; D005260:Female; D020558:GTP Phosphohydrolases / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C489578:Rhog protein, rat; D020558:GTP Phosphohydrolases