Isolation and characterization of highly liganded protein from brewer's barley grain.
Two basic proteins, HLP-1 and HLP-2, were isolated from brewer's barley grain (Hordeum vulgare L.) and characterized as glycoproteins with molecular masses of 16 and 13 kDa and pI values of 7.4 and 8.8, respectively. They could bind sugars, metal ions, and both hydrophobic and hydrophylic molecules of low molecular mass. These characteristics may be related to their potential plant-protecting role.
Gorjanović
Stanislava
S
Institute of General and Physical Chemistry, Belgrade, Yugoslavia. inzenjer@eunet.yu
Cvetković
Ana
A
Suznjević
Desanka
D
Beljanski
Milos
M
Vrvić
Miroslav
M
Hranisavljević
Jovan
J
D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D001467:Hordeum / Q000737:chemistry*; D008024:Ligands; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D011485:Protein Binding; D012639:Seeds / Q000737:chemistry*
D006023:Glycoproteins; D008024:Ligands; D010940:Plant Proteins