title: High efficient encapsulation of plasmid DNA in PLGA microparticles by organic phase self-emulsification.
abstract: To overcome the drawbacks of encapsulating plasmid DNA (pDNA) in poly (D,L-lactic-co-glycolic acid) (PLGA) by water-in-oil-in-water double-emulsion solvent-evaporation method, we have developed a novel procedure for encapsulating pDNA in PLGA microparticles called DNA organic phase self-emulsification (DOPSM). This method was based on both the extraction plasmid DNA from aqueous phase into organic phase and the spontaneous emulsification DNA in organic phase by solvent diffusion method. The efficiency of extraction plasmid DNA into organic phase is 99% and the concentration of pDNA in organic phase is up to 2.4 mg/ml. The efficiency of microencapsulation of plasmid DNA in PLGA is up to 76% and can be enhanced by lowering the pH of aqueous solution of emulsion. The microparticles size of PLGA of pDNA is in a narrow range of 1-2 microm. This procedure does not involve the high mechanical energy to emulsify which may damage the integrity of pDNA. This method can be applied to encapsulate the pDNA into microparticles of other biocompatible polymers with high efficiency.
authors.affiliation: Department of Biological Science and Biotechnology, Tsinghua University, 100084 Beijing, PR China.
authors.forename: F F
authors.initials: FF
authors.lastname: Zhuang
authors.forename: R
authors.initials: R
authors.lastname: Liang
authors.forename: C T
authors.initials: CT
authors.lastname: Zou
authors.forename: H
authors.initials: H
authors.lastname: Ma
authors.forename: C X
authors.initials: CX
authors.lastname: Zheng
authors.forename: M X
authors.initials: MX
authors.lastname: Duan
mesh_terms: D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000295:instrumentation*; D004655:Emulsions / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D008863:Microspheres; D010316:Particle Size; D010957:Plasmids / Q000008:administration & dosage* / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011786:Quality Control
chemical_list: D020099:Coated Materials, Biocompatible; D004655:Emulsions; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid