Cellular distributions of creatine kinase in branchia of euryhaline tilapia (Oreochromis mossambicus).
Although euryhaline teleosts can adapt to environmental fluctuation of salinity, their energy source for responding to changes in salinity and osmolarity remains unclear. This study examines the cellular localization of creatine kinase (CK) expression in branchia of tilapia (Oreochromis mossambicus). Western blot analysis of muscle-type CK (MM form) revealed a high association with salinity changes, but BB and MB forms of CK in the gills of fish adapted to seawater did not change. With the use of immunocytochemistry, three CK isoforms (MM, MB, and BB) were localized in mitochondria-rich (MR) cells and other epithelial cells of tilapia gills. In addition, staining intensity of MM-form CK in MR cells increased after seawater transfer, whereas BB and MB forms did not significantly change. To our knowledge, this work presents the first evidence of CK expression in MR cells of tilapia gills, highlighting the potential role of CK in providing energy for ion transport.
Lin
Li-Yih
LY
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan 100.
Chiang
Chia-Chang
CC
Gong
Hong-Yi
HY
Cheng
Ching-Yi
CY
Hwang
Pung-Pung
PP
Weng
Ching-Feng
CF
D000818:Animals; D001934:Branchial Region / Q000166:cytology* / Q000201:enzymology*; D003402:Creatine Kinase / Q000096:biosynthesis*; D007527:Isoenzymes / Q000096:biosynthesis; D017210:Tilapia
D007527:Isoenzymes; D003402:Creatine Kinase