Inhibitory effect of Artemisia capillaris on ethanol-induced cytokines (TNF-alpha, IL-1alpha) secretion in Hep G2 cells.
A human hepatoma cell line, Hep G2 cell, is reliable for the study of alcohol-induced hepatotoxicity. In this study, we investigated the effect of an aqueous extract of Artemisia capillaris Thunb (Compositae) plant (AC) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. AC (0.5-5 microg/mL) inhibited the secretion of EtOH-induced interluekin-1alpha (IL-1alpha) and tumor necrosis factor-alpha (TNF-alpha). AC also inhibited the EtOH-, IL-1alpha-, and TNF-alpha-induced cytotoxicity. Furthermore, we found that AC inhibited the EtOH-induced apoptosis of Hep G2 cells. These results suggest that AC may prevent the EtOH-induced cytotoxicity through inhibition of the apoptosis of Hep G2 cells.
Koo
Hyun-Na
HN
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, and KI Co. Ltd., Iksan, Jeonbuk, South Korea.
Hong
Seung-Heon
SH
Jeong
Hyun-Ja
HJ
Lee
Eun-Hee
EH
Kim
Nam-Guek
NG
Choi
Su-Deock
SD
Ra
Ki-Woong
KW
Kim
Kang-San
KS
Kang
Byung-Ki
BK
Kim
Jeong-Joong
JJ
Oh
Jeong Guk
JG
Kim
Hyung-Min
HM
D017209:Apoptosis / Q000187:drug effects; D019306:Artemisia; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D008099:Liver / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007375:Interleukin-1; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000431:Ethanol