A new approach based on monitoring of phase formation kinetics for examination of biological particles and cells, using aqueous two phase polymer systems.
An original technique of use of two-phase polymer systems as an analytical research method is described. The technique is based on the absorbance change of two-phase systems in visible spectrum during formation of the phases. Dynamics of this process was demonstrated as the kinetic curves. Addition of studied objects (macromolecules or cells) to the two-phase system modified the shape of the kinetic curve, depending on their individual surface properties. The technique has the following advantages as compared with traditional procedures of the particle surface analysis with the help of two-phase polymer systems: examination of particles with partition coefficients approaching zero; multiple analyses of the same samples; use of interphase as a matrix for study of spontaneous formation of studied particle complexes. The opportunities of the technique were demonstrated in a series of previous authors' works.
Petrov
Yu P
YP
Department of Cell Cultures, Institute of Cytology, Russian Academy of Sciences, Tichoretsky ave. 4, St. Petersburg 194064, Russia. jpetrov@fromru.com
Pinaev
G P
GP
D000199:Actins / Q000737:chemistry; D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003377:Countercurrent Distribution; D003911:Dextrans; D004655:Emulsions; D004912:Erythrocytes / Q000737:chemistry; D007700:Kinetics; D046911:Macromolecular Substances; D010316:Particle Size; D011092:Polyethylene Glycols; D011108:Polymers; D013053:Spectrophotometry; D013499:Surface Properties; D014867:Water
D000199:Actins; D003911:Dextrans; D004655:Emulsions; D046911:Macromolecular Substances; D011108:Polymers; D014867:Water; D011092:Polyethylene Glycols