title: Tightly regulated gene expression system in Salmonella enterica serovar Typhimurium.
abstract: A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P(BAD) control element into the bacterial chromosome allows dynamic control of T7 promoter-driven RNA transcription.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: McKinney
authors.forename: Cecilia
authors.initials: C
authors.lastname: Guerrier-Takada
authors.forename: Jorge
authors.initials: J
authors.lastname: Galán
authors.forename: Sidney
authors.initials: S
authors.lastname: Altman
mesh_terms: D051777:AraC Transcription Factor; D001089:Arabinose / Q000378:metabolism; D001426:Bacterial Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial; D012097:Repressor Proteins / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics*; D014157:Transcription Factors; D014158:Transcription, Genetic; D014764:Viral Proteins
chemical_list: D051777:AraC Transcription Factor; D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D001089:Arabinose; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D005947:Glucose