Ontogenesis of epithelial phosphate transport systems in goats.
The rapid development of precocial goats in the first weeks after birth requires an adequate adaptation of phosphate transport systems to maintain the P homeostasis at each developmental stage. Here we examined the age-related development of Na+-Pi transport systems in small intestines, kidneys, and parotid glands of goats. Kinetic parameters were determined by brush-border membrane vesicle uptake studies, and relative expression of NaPi type II mRNA and protein was recorded by molecular biological methods. High intestinal Pi transport capacity was already present on the first day of life. Within the first 3 wk of life there seemed to be a change in the type of Na+-dependent Pi transporter, and NaPi IIb was expressed increasingly up to the fifth month of life. Renal Na+-Pi transport capacity was also high at birth, and this was associated with high expression levels of NaPi IIa mRNA, indicating the important role of this transporter for renal Pi reabsorption. At weaning an increase in both intestinal and renal Na+-Pi transport balanced the increasing requirements for Pi to establish the endogenous Pi cycle. Salivary Pi concentration and parotid NaPi II mRNA rose markedly to guarantee an adequate Pi supply for rumen microbes. We concluded that the high demand for Pi in young goats was assured by high basal Na+-Pi transport capacity of small intestines and kidney expressed continuously during ontogenesis.
Huber
Korinna
K
Department of Physiology, School of Veterinary Medicine Hannover, D-30173 Hannover, Germany. korinna.huber@tiho-hannover.de
Roesler
Uta
U
Muscher
Alexandra
A
Hansen
Kathrin
K
Widiyono
Irkham
I
Pfeffer
Ernst
E
Breves
Gerhard
G
D000375:Aging; D000595:Amino Acid Sequence; D000818:Animals; D001693:Biological Transport, Active; D005260:Female; D006041:Goats / Q000254:growth & development* / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008297:Male; D008871:Microvilli / Q000378:metabolism; D008969:Molecular Sequence Data; D010306:Parotid Gland / Q000378:metabolism; D028061:Phosphate Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010710:Phosphates / Q000097:blood / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry; D012964:Sodium / Q000378:metabolism
D028061:Phosphate Transport Proteins; D010710:Phosphates; D012333:RNA, Messenger; D012964:Sodium