title: Matrix metalloproteinase-2, membranous type 1 matrix metalloproteinase, and tissue inhibitor of metalloproteinase-2 expression in ectopic and eutopic endometrium.
abstract: OBJECTIVE
  To investigate expression of matrix metalloproteinase-2 (MMP-2), membranous type 1 matrix metalloproteinase (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2) in ectopic and eutopic endometrium from women with and without endometriosis throughout the menstrual cycle.
  
  
  METHODS
  Molecular studies in human tissue.
  
  
  METHODS
  Reproductive immunology laboratory of a university medical center.
  
  
  METHODS
  Fifty-three premenopausal woman (23 with endometriosis and 30 without endometriosis) undergoing laparoscopic surgery. Endometrium and ectopic endometriosis tissue were obtained at the time of surgery.
  
  
  METHODS
  Messenger RNA and protein expression from eutopic and ectopic endometrium was analyzed by using quantitative competitive polymerase chain reaction, zymography, and Western blot assay.
  
  
  RESULTS
  Uterine endometrium from women with endometriosis expressed higher levels of MMP-2 and MT1-MMP and lower levels of TIMP-2 than did endometrium from normal women.
  
  
  CONCLUSIONS
  Eutopic endometrium from patients with endometriosis may be more invasive and prone to peritoneal implantation because of greater expression of MMP-2 and MT1-MMP and lower expression of TIMP-2 messenger RNA, compared with endometrium from women without endometriosis. Thus, increased proteolytic activity may help to explain the invasive factors that result in endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University School of Medicine, Seoul, Korea. hyewon@mm.ewha.ac.kr
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Chung
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Hye-Sung
authors.initials: HS
authors.lastname: Moon
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Hur
authors.forename: Mi Hye
authors.initials: MH
authors.lastname: Park
authors.forename: Yan
authors.initials: Y
authors.lastname: Wen
authors.forename: Mary Lake
authors.initials: ML
authors.lastname: Polan
mesh_terms: D000328:Adult; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004715:Endometriosis / Q000201:enzymology* / Q000601:surgery; D004717:Endometrium / Q000201:enzymology* / Q000601:surgery; D005260:Female; D015870:Gene Expression; D006801:Humans; D010535:Laparoscopy; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000235:genetics*; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000032:analysis / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D019716:Tissue Inhibitor of Metalloproteinase-2; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2