Internal dynamics and ionization states of the macrophage migration inhibitory factor: comparison between wild-type and mutant forms.
The macrophage migration inhibitory factor (MIF) is a cytokine that shares a common structural architecture and catalytic strategy with three isomerases: 4-oxalocrotonate tautomerase, 5-carboxymethyl-2-hydroxymuconate isomerase, and D-dopachrome tautomerase. A highly conserved N-terminal proline acts as a base-acid during the proton transfer reaction catalyzed by these enzymes. Such unusual catalytic strategy appears to be possible only due to the N-terminal proline pK(a) shifted to 5.0-6.0 units. Mutations of this residue result in a significant decrease of the catalytic activity of MIF. Two hypotheses have been proposed to explain the catalytic inefficiency of MIF: the lower basicity of primary amines with regard to secondary ones and the increased flexibility resulting from the replacement of a proline by residues like glycine. To investigate that, we have performed molecular dynamics simulations of MIF wild-type and its mutant P1G, as well as calculated the protonation properties of several mutant forms. It was found that the N-terminal glycine does not show larger fluctuations compared to proline, but the former residue is more exposed to the solvent throughout the simulations. The apparent pK(a) of these residues displays very little change (as expected from the structural rigidity of MIF) and is not significantly affected by the surrounding ionizable residues. Instead, the hydrophobic character of the active site seems to be the main factor in determining the pKa of the N-terminal residue and the catalytic efficiency of MIF.
Soares
T A
TA
Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, Zurich, Switzerland. tsoares@igc.phys.chem.ethz.ch
Lins
R D
RD
Straatsma
T P
TP
Briggs
J M
JM
D001665:Binding Sites; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007477:Ions; D008263:Macrophage Migration-Inhibitory Factors / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D013816:Thermodynamics
D007477:Ions; D008263:Macrophage Migration-Inhibitory Factors