[Development of quantitative immunoenzyme and radioimmunologic analysis of lambda-free immunoglobulin light chains].
New quantitative techniques of radioimmunoassay (RIA) and enzyme immunoassay (EIA) were developed for determination of free light lambda-chains of immunoglobulins (immunometric sandwich-like variants) in biological fluids using two types of monoclonal specific antibodies (MAB-1 and MAB-2) to different epitopes of the antigen molecule: MAB-1 were immobilized on a solid phase (polystyrene beads), whereas MAB-2 were labeled with iodine or with the enzyme. The test systems prepared can be used for determination of concentrations from 25 to 1000 ng/ml, are very sensitive (25 ng/ml), and the analysis time is 5 h. The two methods were compared, and their clinical and diagnostic validity was evaluated in patients with various diseases associated with disorders in the antibody synthesis by the immune system.
Piven'
N V
NV
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, 220141 Belarus. piven@ns.iboch.ac.by
Goncharik
A V
AV
Reznikov
Iu P
IuP
Shkumatova
G A
GA
Kul'bakova
A I
AI
D000911:Antibodies, Monoclonal / Q000276:immunology; D007124:Immunoenzyme Techniques / Q000379:methods*; D007147:Immunoglobulin Light Chains / Q000737:chemistry* / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D007147:Immunoglobulin Light Chains