Common gene targets of Ginkgo biloba extract (EGb 761) in human tumor cells: relation to cell growth.
The standardized extract of Ginkgo biloba leaves (EGb 761) has been shown to inhibit aggressive human breast cancer cell proliferation both in vitro and in vivo. These results were extended to human glioma and hepatoma cells in vitro suggesting that EGb 761 may have a more widespread application for tumor growth control. To understand the mechanism by which EGb 761 acts to inhibit cell proliferation, we investigated the effects of EGb 761 on human breast cancer, glioma and hepatoma cell transcriptomes by means of various large-scale DNA array techniques. The data presented focus on genes regulated by EGb 761 that are common to the three tumor cell types and for which the data were verified by two different types of DNA microarray and/or RNA (Northern) blot analysis and real-time quantitative PCR. These results could therefore help elucidate the mechanism of cytostatic action of EGb 761 and identify genes important for tumor growth.
Li
Wenping
W
Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20057 USA.
Pretner
Ewald
E
Shen
Liya
L
Drieu
Katy
K
Papadopoulos
Vassilios
V
D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics*; D002352:Carrier Proteins / Q000187:drug effects*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D020441:Ginkgo biloba; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000494:pharmacology*; D039103:Poly(A)-Binding Protein II / Q000187:drug effects; D018079:Receptors, GABA / Q000187:drug effects*; D014407:Tumor Cells, Cultured; D000096983:Ginkgo Extract
D002352:Carrier Proteins; D010936:Plant Extracts; D039103:Poly(A)-Binding Protein II; D018079:Receptors, GABA; C499229:TSPO protein, human; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract