title: Immunolocalization of cholecystokinin-2 receptors in rat gastric mucosa.
abstract: BACKGROUND
  Gastrin exerts trophic effects on the gastric mucosa by mechanisms not yet completely elucidated. Our aim was to localize the cholecystokinin-2 (CCK2) receptor in epithelial cells of foetal and adult rat stomachs in order to determine the cell types that are directly affected by gastrin.
  
  
  METHODS
  Gastric tissue was subjected to indirect double immunofluorescence staining with antiserum against the C-terminal decapeptide of the CCK2 receptor and antibodies against 5' bromo-2-deoxyuridine, which had been injected into the rats I h before they were killed, the acid pump H,K-ATPase, the membrane-cytoskeletal linker ezrin, pepsin/pepsinogen or histidine decarboxylase.
  
  
  RESULTS
  Undifferentiated foetal gastric epithelial cells expressed CCK2 receptors, whereas stem cells of adult gastric glands did not exhibit immunoreactivity. However, other epithelial cells in the progenitor zone of adult gastric glands did express CCK2 receptors. Some of these cells were faintly stained for H,K-ATPase; pepsin/pepsinogen was also detected in this region. Parietal cells in the isthmus/pit region of the glands contained ezrin, and some showed weak immunoreactivity for the CCK2 receptor. As expected, enterochromaffin-like cells also expressed CCK2 receptors.
  
  
  CONCLUSIONS
  Our findings are consistent with the hypothesis that a CCK2 receptor mediates direct effects of gastrin on gastric epithelial cells during both stomach organogenesis and adult life.
authors.affiliation: Dept of Biomedicine and Surgery, Faculty of Health Sciences, Linköping University, Sweden. karin.tommeras@medisin.ntnu.no
authors.forename: K
authors.initials: K
authors.lastname: Tømmerås
authors.forename: P
authors.initials: P
authors.lastname: Hammer
authors.forename: F
authors.initials: F
authors.lastname: Sundler
authors.forename: K
authors.initials: K
authors.lastname: Borch
authors.forename: S
authors.initials: S
authors.lastname: Mårdh
authors.forename: J L
authors.initials: JL
authors.lastname: Cabero
mesh_terms: D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D005753:Gastric Mucosa / Q000196:embryology / Q000378:metabolism*; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism; D008297:Male; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin / Q000378:metabolism*
chemical_list: D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin; D017506:H(+)-K(+)-Exchanging ATPase