title: The efficacy of Salenvac, a Salmonella enterica subsp. Enterica serotype Enteritidis iron-restricted bacterin vaccine, in laying chickens.
abstract: The protective effect of two vaccination regimes using Salenvac, a commercially available iron-restricted Salmonella enterica subsp. Enterica serotype Enteritidis PT4 bacterin vaccine, was verified in laying birds. Immunization was intramuscular at 1 day old and again at 4 weeks of age (V2), or at 1 day and 4 weeks with a third dose at 18 weeks of age (V3). Challenge S. Enteritidis (5 to 7.5); x 10(7) colony forming units) was given intravenously at 8, 17, 23, 30 and 59 weeks of age. For all age groups, both vaccination regimes reduced significantly the number of tissues and faecal samples that were culture positive for the challenge strain. For laying birds, fewer eggs (P < 0.001) were culture positive for S. Enteritidis after challenge from vaccinated laying birds (56/439 batches of eggs) than unvaccinated birds (99/252 batches). The data give compelling evidence that the vaccine is efficacious and may contribute to the reduction of layer infection and egg contamination.
authors.affiliation: Department of Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK. M.J.Woodward@vla.defra.gsi.gov.uk
authors.forename: M J
authors.initials: MJ
authors.lastname: Woodward
authors.forename: G
authors.initials: G
authors.lastname: Gettinby
authors.forename: M F
authors.initials: MF
authors.lastname: Breslin
authors.forename: J D
authors.initials: JD
authors.lastname: Corkish
authors.forename: S
authors.initials: S
authors.lastname: Houghton
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D002645:Chickens; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary; D005260:Female; D010058:Oviposition / Q000502:physiology*; D011201:Poultry Diseases / Q000453:epidemiology; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000517:prevention & control; D012477:Salmonella enteritidis / Q000276:immunology*; D013997:Time Factors; D014611:Vaccination / Q000009:adverse effects / Q000379:methods / Q000662:veterinary
chemical_list: D001428:Bacterial Vaccines