[The experimental study of culture in vitro of fibroblasts seeded onto human amnion extracellular matrix (HA-ECM)].
OBJECTIVE
The aim of this experiment is to find proper cell carrier for skin tissue engineering.


METHODS
Various concentration of fibroblasts were seeded onto HA-ECM and cultured in vitro. The performance of cells' growth, array, adhesion and collagen secretion on HA-ECM was observed with light microscope and transmission electron microscope.


RESULTS
The fusiform fibroblasts oriented radiantly or longitudinally and closely packed onto the HA-ECM, they attached firmly and proliferated to confluence on the stromal surface of HA-ECM.


CONCLUSIONS
The optimal cell concentration is 3.5 x 10(6)/ml, HA-ECM is ideal carrier for fibroblasts because of its excellent scaffold and diffusion characteristics. Futhermore, it has the bioactive molecules such as fibronectin and laminin which play an important role in fibroblasts attachment and proliferation on HA-ECM.
He
Qingyi
Q
Department of Orthopaedics, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Chen
Bingli
B
Wang
Zhibiao
Z
Li
Qihong
Q
D000650:Amnion / Q000166:cytology*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D005109:Extracellular Matrix; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D006416:Hematoxylin; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011817:Rabbits
D006416:Hematoxylin