title: Hydrogen peroxide sensitive amperometric biosensor based on horseradish peroxidase entrapped in a polypyrrole electrode.
abstract: The enzyme horseradish peroxidase (HRP) has been entrapped in situ by electropolymerization of pyrrole onto a platinum electrode. The latter was previously coated by a polypyrrole layer for better adhesion of the biocatalyst film and in order to avoid the enzyme folding onto the Pt electrode. The biosensor allowed the determination of hydrogen peroxide in the concentration range comprised between 4.9 x 10(-7) and 6.3 x 10(-4) M. The biosensor retained more than 90% of its original activity after 35 days of use.
authors.affiliation: Institut de Pharmacie, Université Libre de Bruxelles, Campus Plaine, CP 205/6, 1050 Brussels, Belgium.
authors.forename: S Serradilla
authors.initials: SS
authors.lastname: Razola
authors.forename: B Lopez
authors.initials: BL
authors.lastname: Ruiz
authors.forename: N Mora
authors.initials: NM
authors.lastname: Diez
authors.forename: H B
authors.initials: HB
authors.lastname: Mark
authors.forename: J M
authors.initials: JM
authors.lastname: Kauffmann
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D019544:Equipment Failure Analysis; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010984:Platinum; D011108:Polymers; D011758:Pyrroles; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D010984:Platinum; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase