title: Response of in vivo protein synthesis in T lymphocytes and leucocytes to an endotoxin challenge in healthy volunteers.
abstract: In vivo determination of protein synthesis in immune cells reflects metabolic activity and immunological activation. An intravenous injection of endotoxin to healthy volunteers was used as a human sepsis model, and in vivo protein synthesis of T lymphocytes and leucocytes was measured. The results were related to plasma concentrations of selected cytokines, peripheral cell counts and subpopulations of immune cells. The subjects (n = 8 + 8) were randomized to an endotoxin (4 ng/kg) or a saline group. In vivo protein synthesis was determined twice: before and 1-2.5 h after the endotoxin/saline injection. Protein synthesis decreased in isolated T lymphocytes, but increased in leucocytes. Plasma levels of TNF-alpha, IL-8, IL-6, IL-1 ra and IL-10 were elevated, whereas IL-2 and IFN-gamma, produced predominantly by T lymphocytes, did not change in response to endotoxin. Neutrophils increased, whereas lymphocytes and monocytes decreased 2.5 h after the endotoxin injection. Flow cytometry revealed a drop in total CD3+ T lymphocytes and CD56+ natural killer cells, accompanied by an increase in CD15+ granulocytes. In summary, in vivo protein synthesis decreased in T lymphocytes, while the total leucocyte population showed a concomitant increase immediately after the endotoxin challenge. The changes in protein synthesis were accompanied by alterations in immune cell subpopulations and in plasma cytokine levels.
authors.affiliation: Department of Anaesthesiology and Intensive Care, Huddinge University Hospital, Karolinska Institutet, Stockholm, Sweden. anna.januszkiewicz@anaesth.hs.sll.se
authors.forename: A
authors.initials: A
authors.lastname: Januszkiewicz
authors.forename: K
authors.initials: K
authors.lastname: Loré
authors.forename: P
authors.initials: P
authors.lastname: Essén
authors.forename: B
authors.initials: B
authors.lastname: Andersson
authors.forename: M A
authors.initials: MA
authors.lastname: McNurlan
authors.forename: P J
authors.initials: PJ
authors.lastname: Garlick
authors.forename: O
authors.initials: O
authors.lastname: Ringdén
authors.forename: J
authors.initials: J
authors.lastname: Andersson
authors.forename: J
authors.initials: J
authors.lastname: Wernerman
mesh_terms: D000328:Adult; D017252:CD3 Complex / Q000032:analysis; D019002:CD56 Antigen / Q000032:analysis; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004731:Endotoxins / Q000494:pharmacology*; D006801:Humans; D007694:Killer Cells, Natural / Q000737:chemistry; D007958:Leukocyte Count; D007962:Leukocytes / Q000378:metabolism*; D016256:Lewis X Antigen / Q000032:analysis; D008297:Male; D009504:Neutrophils / Q000737:chemistry; D014176:Protein Biosynthesis; D018805:Sepsis / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D017252:CD3 Complex; D019002:CD56 Antigen; D016207:Cytokines; D004731:Endotoxins; D016256:Lewis X Antigen