title: [Association of immune response to hepatitis B vaccine with HLA-DRB1*02, 07, 09 genes in the population of Han nationality in Guangdong Province].
abstract: OBJECTIVE
  To study the association between immune response to hepatitis B vaccine and HLA-DRB1*02, 07, 09 genes in the population of Han nationality in Guangdong Province.
  
  
  METHODS
  A total of 1 400 healthy college students of Han nationality from Guangdong Province were recruited in this study, who received standard courses of vaccination with recombinant hepatitis B virus vaccine at month 0, 1 and 6. Eight weeks after the final vaccination, the serum levels of anti-HBs antibody was examined with enzyme-linked immunosorbent assay (ELISA). Detection of HLA-DRB1*02, 07, 09 alleles were performed with PCR using sequence-specific primers in 118 individuals who failed to produce or produced insufficient antibodies (nonresponders or weak responders), 100 normal responders and 45 strong responders.
  
  
  RESULTS
  The frequency of HLA-DRB1*07 in nonresponders or weak responders was significantly higher than that in normal or strong responders (15.25% vs 3.44%, P<0.05), while the frequency of HLA-DRB1*02 was much lower (5.08% vs 17.9%, P<0.05). The frequency of HLA-DRB1*09 showed no obvious difference between the 2 groups (19.49% vs 17.9%, P>0.05).
  
  
  CONCLUSIONS
  Failure or deficiency in the response to HBV vaccine is associated with the HLA-DRB1*07 gene, while HLA-DRB1*02 gene is related to the normal vaccination process or overreaction. Definite relation of HLA-DRB1*09 to the response to HBV vaccine is not found.
authors.affiliation: Department of Infectious Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. gzqy1226@21cn.com
authors.forename: Yi
authors.initials: Y
authors.lastname: Qian
authors.forename: Lian
authors.initials: L
authors.lastname: Zhang
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Liang
authors.forename: Jin-Lin
authors.initials: JL
authors.lastname: Hou
authors.forename: Kang-Xian
authors.initials: KX
authors.lastname: Luo
mesh_terms: D000328:Adult; D002681:China; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005828:Genetics, Population; D006684:HLA-DR Antigens / Q000276:immunology*; D059811:HLA-DRB1 Chains; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D007109:Immunity; D008297:Male
chemical_list: D017931:DNA Primers; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D017325:Hepatitis B Vaccines