Evaluation of support materials for the surface immobilization and decoloration of amaranth by Trametes versicolor.
The ability of Trametes versicolor ATCC 20869 to colonize several natural and synthetic materials (wheat straw, jute, hemp, maple woodchips, and nylon and polyethylene teraphthalate fibers) and to subsequently decolorize amaranth was evaluated. Jute was found to be the best support material as T. versicolor grew well on it without color leaching from the support and without loss of the jute's integrity over a 4 week period. The fungus immobilized on jute, straw and hemp decolorized amaranth (50 mg l(-1)) at a rate of about 5 mg l(-1) h(-1) without glucose being added. When 1 g l(-1) glucose was added, the dye was degraded more quickly (about 8 mg l(-1) h(-1)). Decoloration did not occur in a suspension culture without glucose. As the number of decoloration cycles increased, the rate of decoloration decreased. This rate was restored to its original level after the biomass was incubated in fresh growth medium for 5 days. With all immobilization supports, the toxicity of the medium before and after decoloration was the same or lower.
Shin
M
M
Department of Chemical Engineering, Queen's University, Kingston, Ontario, K7L 3N6, Canada.
Nguyen
T
T
Ramsay
J
J
D000548:Amaranth Dye / Q000378:metabolism*; D001487:Basidiomycota / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D018914:Cells, Immobilized; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism / Q000633:toxicity; D005069:Evaluation Studies as Topic; D005947:Glucose / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism; D014866:Waste Products
D004396:Coloring Agents; D003470:Culture Media; D014866:Waste Products; D000548:Amaranth Dye; D005947:Glucose