title: Further evidence for the importance of lipid bilayers in the interaction between lactate dehydrogenase and phosphatidylserine.
abstract: Lactate dehydrogenase (LDH) is one of the glycolytic enzymes, which have been proved to have the capability to reverse non-specific adsorption on cellular membranous structures in vitro, as well as on the structural proteins of the contractile system of muscle cells. It has been suggested that this binding may play a physiological role, as it alters the enzyme's kinetic properties. Our previous studies on this enzyme showed that its interaction with some anionic phospholipids reveals similar characteristics and similar effect on the activity of the enzyme to those which had been observed for the interaction with membranous structures. Disruption of the lipid bilayers by nonionic detergent (Tween 20) restored the enzyme activity inhibited by the presence of phosphatidylserine (PS) liposomes. In this study, we used the measurement of enzyme tryptophanyl fluorescence spectra to monitor the interaction and possible changes in the enzyme conformation. The investigation provided further evidence of the importance of the bilayer structure in this interaction. Similarly to the effect on the activity of the enzyme, the addition of Tween 20 diminishes the quenching of the LDH tryptophanyl fluorescence, and finally completely restores the fluorescence.
authors.affiliation: Department of Medical Biochemistry, Wrocław Medical University, Wrocław, Poland. terlecki@biochem.am.wroc.pl
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Terlecki
authors.forename: Jan
authors.initials: J
authors.lastname: Gutowicz
mesh_terms: D000818:Animals; D006019:Glycolysis; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008051:Lipid Bilayers; D018482:Muscle, Skeletal / Q000201:enzymology; D010718:Phosphatidylserines / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013552:Swine
chemical_list: D008051:Lipid Bilayers; D010718:Phosphatidylserines; D007770:L-Lactate Dehydrogenase