Effect of N-desulfated heparin on hepatic/renal ischemia reperfusion injury in rats.
OBJECTIVE
To investigate the effect of N-desulfated heparin on hepatic/renal ischemia and reperfusion injury in rats.


METHODS
Using rat models of 60 minutes hepatic or renal ischemia followed by 1 h,3 h,6 h and 24 h reperfusion,animals were randomly divided into following groups,the sham operated controls,ischemic group receiving only normal saline,and treated group receiving N-desulfated heparin at a dose of 12 mg/kg at 5 minutes before reperfusion. P-selectin expression was detected in hepatic/renal tissues with immunohistochemistry method.


RESULTS
P-selectin expression, serum ALT, AST, BUN and Cr levels were significantly increased during 60 minute ischemia and 1 h, 3 h, 6 h and 24 h reperfusion,while the increment was significantly inhibited,and hepatic/renal pathology observed by light microscopy was remarkably improved by treatment with the N-desulfated heparin. Furthermore,the heparin was found no effects on PT and KPTT.


CONCLUSIONS
P-selectin might mediate neutrophil infiltration and contribute to hepatic/renal ischemia and reperfusion. The N-desulfated heparin might prevent hepatic/renal damage induced by ischemia and reperfusion injury without significant anticoagulant activity.
Zhou
Tong
T
Department of Nephrology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025,China. zhoutong_cn@hotmail.com
Chen
Jin-Lian
JL
Song
Wei
W
Wang
Feng
F
Zhang
Ming-Jun
MJ
Ni
Pei-Hua
PH
Geng
Jian-Guo
JG
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D001777:Blood Coagulation / Q000187:drug effects; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D006493:Heparin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D007668:Kidney / Q000737:chemistry / Q000473:pathology*; D008099:Liver / Q000737:chemistry / Q000473:pathology*; D008297:Male; D019007:P-Selectin / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology*
D000925:Anticoagulants; D019007:P-Selectin; C044153:heparin, N-desulfated; D006493:Heparin; D003404:Creatinine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase