Isolation and characterization of cold-shock domain protein genes, Oryzias latipes Y-box protein 2 (OlaYP2) and Fugu rubripes Y-box protein 1 (FruYP1), in medakafish and pufferfish.
The Y-box protein (YP) family shares a nucleic acid binding domain, called cold-shock domain, that has been evolutionarily highly conserved from bacteria to human. The different YPs identified so far in vertebrates are thought to function as transcriptional activators, transcriptional repressors and/or translational repressors. Medakafish and pufferfish are very suitable vertebrate models for the study of developmental genetics and comparative genomics, respectively. Here we report the isolation of two teleost YP genes, medakafish Oryzias latipes (Ola)YP2 and Fugu rubripes (Fru)YP1, which are expressed in multiple tissues. Phylogenetic analysis demonstrated that OlaYP2 and FruYP1 belong to different subclasses of the cold-shock domain protein genes. Future studies in suitable model systems, like the medaka for developmental biology and Fugu for evolutionary genomics, are expected to contribute to our understanding of YPs.
Zend-Ajusch
Enchshargal
E
Research School of Biological Sciences, Australian National University, Canberra, ACT 2601, Australia.
Hornung
Ute
U
Burgtorf
Carola
C
Lütjens
Götz
G
Shan
Zhihong
Z
Schartl
Manfred
M
Haaf
Thomas
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015870:Gene Expression; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D009990:Oryzias / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D023741:Takifugu / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D018076:DNA, Complementary; D014157:Transcription Factors