Trefoil peptide expression and goblet cell number in rat intestine: effects of KGF and fasting-refeeding.
The trefoil factor family peptides TFF1, TFF2, and TFF3 are important for gut mucosal protection and restitution. Keratinocyte growth factor (KGF) stimulates proliferation and differentiation of epithelial cells with potent effects on goblet cells. To investigate interactions between food intake and KGF, rats were fed ad libitum (control), fasted for 72 h, or fasted for 72 h and then refed for 72 h with or without KGF (3 mg. kg(-1). day(-1)). With fasting, goblet cell number in duodenum increased, TFF3 mRNA in duodenum and jejunum decreased, and TFF3 protein did not change or increased. KGF during fasting stimulated colonic growth, normalized TFF3 mRNA in duodenum and jejunum, and broadly upregulated gut goblet cell number and TFF3 protein expression. With fasting-refeeding, KGF increased small bowel and colonic mucosal growth, goblet cell number, and TFF3 protein but had variable effects on TFF3 mRNA. KGF induced TFF2 mRNA and protein in duodenum and jejunum with both nutritional regimens. We conclude that nutrient availability modifies rat intestinal goblet cell number, TFF3 mRNA, and the gut-trophic effects of KGF in a region-specific manner. KGF enhances TFF2 expression in proximal small bowel and increases goblet cell number and TFF3 protein content throughout the intestine independent of food intake.
Fernandez-Estivariz
Concepcion
C
Department of Medicine, Laboratory Medicine, and Biochemistry, Emory University School of Medicine; Nutrition and Health Sciences Program, Emory University, Atlanta, Georgia 30322, USA.
Gu
Li H
LH
Gu
Liang
L
Jonas
Carolyn R
CR
Wallace
Timothy M
TM
Pascal
Robert R
RR
Devaney
Kathryn L
KL
Farrell
Catherine L
CL
Jones
Dean P
DP
Podolsky
Daniel K
DK
Ziegler
Thomas R
TR
D000818:Animals; D015153:Blotting, Western; D001835:Body Weight; D002452:Cell Count; D004435:Eating; D005215:Fasting / Q000502:physiology*; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005508:Food Deprivation; D005786:Gene Expression Regulation / Q000187:drug effects*; D020397:Goblet Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D006133:Growth Substances / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008297:Male; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3
C496455:Fgf7 protein, rat; D006133:Growth Substances; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; D012333:RNA, Messenger; C099037:TFF3 protein, rat; C000604008:Tff2 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors