title: Contribution of Kv4 channels toward the A-type potassium current in murine colonic myocytes.
abstract: A rapidly inactivating K(+) current (A-type current; I(A)) present in murine colonic myocytes is important in maintaining physiological patterns of slow wave electrical activity. The kinetic profile of colonic I(A) resembles that of Kv4-derived currents. We examined the contribution of Kv4 alpha-subunits to I(A) in the murine colon using pharmacological, molecular and immunohistochemical approaches. The divalent cation Cd(2+) decreased peak I(A) and shifted the voltage dependence of activation and inactivation to more depolarized potentials. Similar results were observed with La(3+). Colonic I(A) was sensitive to low micromolar concentrations of flecainide (IC(50) = 11 microM). Quantitative PCR indicated that in colonic and jejunal tissue, Kv4.3 transcripts demonstrate greater relative abundance than transcripts encoding Kv4.1 or Kv4.2. Antibodies revealed greater Kv4.3-like immunoreactivity than Kv4.2-like immunoreactivity in colonic myocytes. Kv4-like immunoreactivity was less evident in jejunal myocytes. To address this finding, we examined the expression of K(+) channel-interacting proteins (KChIPs), which act as positive modulators of Kv4-mediated currents. Qualitative PCR identified transcripts encoding the four known members of the KChIP family in isolated colonic and jejunal myocytes. However, the relative abundance of KChIP transcript was 2.6-fold greater in colon tissue than in jejunum, as assessed by quantitative PCR, with KChIP1 showing predominance. This observation is in accordance with the amplitude of the A-type current present in these two tissues, where colonic myocytes possess densities twice that of jejunal myocytes. From this we conclude that Kv4.3, in association with KChIP1, is the major molecular determinant of I(A) in murine colonic myocytes.
authors.affiliation: Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA.
authors.forename: Gregory C
authors.initials: GC
authors.lastname: Amberg
authors.forename: Sang Don
authors.initials: SD
authors.lastname: Koh
authors.forename: William J
authors.initials: WJ
authors.lastname: Hatton
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Murray
authors.forename: Kevin
authors.initials: K
authors.lastname: Monaghan
authors.forename: Burton
authors.initials: B
authors.lastname: Horowitz
authors.forename: Kenton M
authors.initials: KM
authors.lastname: Sanders
mesh_terms: D000818:Animals; D002412:Cations / Q000378:metabolism; D003106:Colon / Q000166:cytology / Q000378:metabolism*; D004553:Electric Conductivity; D005424:Flecainide / Q000494:pharmacology; D007583:Jejunum / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D024642:Potassium Channels, Voltage-Gated; D020033:Protein Isoforms / Q000378:metabolism; D051672:Shal Potassium Channels
chemical_list: D002412:Cations; C497016:Kcnd1 protein, mouse; C497019:Kcnd2 protein, mouse; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D020033:Protein Isoforms; D051672:Shal Potassium Channels; D005424:Flecainide