Cloning and sequences of beta-actin genes from Rhodeus notatus and the silver carp Hypophthalmichthys molitrix (cyprinidae) and the phylogeny of Cyprinid fishes inferred from beta-actin genes.
Complete beta-actin genes from Rhodeus notatus and Hypophthalmichthys molitrix (silver carp) were amplified by polymerase chain reaction and cloned into pCRII vector for further characterization. The beta-actin genes of these two fishes were 1718 and 1755 bp in sequence length, respectively, and both of them contained 5 exons and 4 introns. The exon and intron boundaries were identified with the GT/AG rule. The phylogenetic implications of beta-actin gene in the family Cyprinidae were shortly discussed.
Hwang
Ui-Wook
UW
Department of Biology, Teachers College, Kyungpook National University, Taegu 702-701, South Korea.
Han
Myung-Soo
MS
Kim
Ii-Chan
IC
Lee
Yong-Sung
YS
Aoki
Yasunobu
Y
Lee
Jae-Seong
JS
D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D019143:Evolution, Molecular; D005432:Flounder / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny
D000199:Actins