Impediometric detection of Campylobacter coli.
The rapid automated bacterial impedance technique (RABIT) was examined as a method for the detection of two wild-type isolates of Campylobacter coli in broth media. Both isolates failed to produce a change in impedance that was sufficient for detection in any combination of six nonselective basal broth media, including Mueller-Hinton broth, nutrient broth no. 2, brain heart infusion broth supplemented with yeast extract (0.5% [wt/vol]), brucella broth, Campy broth supplemented with yeast extract (0.5% [wt/voll), and Whitley impedance broth, at 37 and 42 degrees C. Although the strains did proliferate in the media, changes in conductivity were very small (ranging from 0 to 1,000 microS) and were not significantly greater than the drift in conductance observed in the control broth medium. Additional work is therefore required to define a nonionic growth substrate that will produce charged ions upon metabolism that are detectable by RABIT.
Moore
John E
JE
Department of Food Science (Food Microbiology), The Queen's University of Belfast, Northern Ireland. jemoore@niphl.dnet.co.uk
Madden
Robert H
RH
D001431:Bacteriological Techniques; D017000:Campylobacter coli / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D017097:Electric Impedance; D005516:Food Microbiology
D003470:Culture Media