Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue.
The structurally homologous mononuclear iron and manganese superoxide dismutases (FeSOD and MnSOD, respectively) contain a highly conserved glutamine residue in the active site which projects toward the active-site metal centre and participates in an extensive hydrogen bonding network. The position of this residue is different for each SOD isoenzyme (Q69 in FeSOD and Q146 in MnSOD of Escherichia coli). Although site-directed mutant enzymes lacking this glutamine residue (FeSOD[Q69G] and MnSOD[Q146A]) demonstrated a higher degree of selectivity for their respective metal, they showed little or no activity compared with wild types. FeSOD double mutants (FeSOD[Q69G/A141Q]), which mimic the glutamine position in MnSOD, elicited 25% the activity of wild-type FeSOD while the activity of the corresponding MnSOD double mutant (MnSOD[G77Q/Q146A]) increased to 150% (relative to wild-type MnSOD). Both double mutants showed reduced selectivity toward their metal. Differences exhibited in the thermostability of SOD activity was most obvious in the mutants that contained two glutamine residues (FeSOD[A141Q] and MnSOD[G77Q]), where the MnSOD mutant was thermostable and the FeSOD mutant was thermolabile. Significantly, the MnSOD double mutant exhibited a thermal-inactivation profile similar to that of wild-type FeSOD while that of the FeSOD double mutant was similar to wild-type MnSOD. We conclude therefore that the position of this glutamine residue contributes to metal selectivity and is responsible for some of the different physicochemical properties of these SODs, and in particular their characteristic thermostability.
Hunter
Thérèse
T
Department of Physiology and Biochemistry, University of Malta, Msida, Malta.
Bannister
Joe V
JV
Hunter
Gary J
GJ
D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D004795:Enzyme Stability / Q000502:physiology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D005973:Glutamine / Q000737:chemistry*; D005982:Glutathione Transferase / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010269:Paraquat / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D019810:Sodium Azide / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics; D013696:Temperature
D016877:Oxidants; D011993:Recombinant Fusion Proteins; D005973:Glutamine; D008345:Manganese; D019810:Sodium Azide; D006861:Hydrogen Peroxide; D007501:Iron; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D010269:Paraquat