title: Organ heterogeneity of host-derived matrix metalloproteinase expression and its involvement in multiple-organ metastasis by lung cancer cell lines.
abstract: Cancer metastasis is tightly regulated by the interaction of tumor cells and host organ microenvironments. Matrix metalloproteinases (MMPs), produced by both tumor cells and host stromal cells, play a central role in tumor invasion and angiogenesis. We determined whether metastatic potential of lung cancer to multiple organs is dependent solely on the expression of MMPs by tumor cells, using two metastasis models of human lung cancer cell lines expressing various levels of MMPs and a MMP inhibitor (ONO-4817). In the lung metastasis model, tumor cells (PC14, PC14PE6, H226, A549) inoculated i.v. into nude or SCID mice metastasized only in the lung. In the multiple-organ metastasis model, tumor cells (RERF-LC-AI, SBC-3/DOX, H69/VP, which express low levels of MMPs) inoculated i.v. into natural killer cell-depleted SCID mice metastasized into the liver, kidneys, and systemic lymph nodes. Film in situ zymography analysis revealed that the nontumor parenchyma of the lung had no gelatinolytic activity, whereas gelatinolytic activity of the liver and kidney was high and low, respectively. In the lung metastasis model, gelatinolytic activity of lung nodules directly correlated with the in vitro expression of MMP-2 and MMP-9 by tumor cells. Inhibition of MMP activity by ONO-4817 suppressed lung metastasis by the cell lines that expressed MMPs, but not those that did not express MMP, via the inhibition of MMP activity of lung tumors. In the multiple-organ metastasis model, liver parenchyma, but not liver nodules, showed gelatinolytic activity. The MMP inhibition reduced metastasis to the liver, but not to the kidney or lymph nodes, via inhibition of MMP activity of liver parenchyma. These findings suggest that MMP expression varies among the host organ microenvironments and that stromal MMPs may promote metastasis of lung cancer. Therefore, antimetastatic effects based on MMP inhibition may be dependent on MMPs derived not only from tumor cells but also from organ-specific microenvironments.
authors.affiliation: Department of Internal Medicine and Molecular Therapeutics, University of Tokushima School of Medicine, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Shiraga
authors.forename: Seiji
authors.initials: S
authors.lastname: Yano
authors.forename: Akihiko
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Ogawa
authors.forename: Hisatsugu
authors.initials: H
authors.lastname: Goto
authors.forename: Toyokazu
authors.initials: T
authors.lastname: Miki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Miki
authors.forename: Helong
authors.initials: H
authors.lastname: Zhang
authors.forename: Saburo
authors.initials: S
authors.lastname: Sone
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology / Q000556:secondary; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000473:pathology / Q000556:secondary; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology*; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000096:biosynthesis / Q000378:metabolism*; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis; D010647:Phenyl Ethers / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D007527:Isoenzymes; D061965:Matrix Metalloproteinase Inhibitors; C414368:N-hydroxy-5-ethoxymethyloxy-2-methyl-4-(4-phenoxybenzoyl)aminopentanamide; D010647:Phenyl Ethers; D011480:Protease Inhibitors; D020782:Matrix Metalloproteinases