title: Tandem genomic arrangement of a G protein (Gna15) and G protein-coupled receptor (s1p(4)/lp(C1)/Edg6) gene.
abstract: A genomic analysis of the s1p(4)/lp(C1)/Edg6 mouse sphingosine-1-phosphate (S1P) G protein-coupled receptor gene revealed it to be located on central chromosome 10 and to consist of two exons with an intronless coding region. Surprisingly, we found the gene encoding the promiscuously coupling G(alpha15) protein (Gna15) located in tandem just upstream, an arrangement conserved in the human genome (on chromosome 19p13.3). Given that Northern blots demonstrated similar tissue distributions of the mouse s1p(4) and Gna15 transcripts, we propose that transcription of the two genes may be under control of the same enhancer elements and that their protein products may couple in vivo.
authors.affiliation: Department of Pharmacology, School of Medicine, University of California at San Diego, La Jolla, CA 92093-0636, USA.
authors.forename: James J A
authors.initials: JJ
authors.lastname: Contos
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Ye
authors.forename: Valerie P
authors.initials: VP
authors.lastname: Sah
authors.forename: Jerold
authors.initials: J
authors.lastname: Chun
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D019992:Databases as Topic; D004742:Enhancer Elements, Genetic; D005091:Exons; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D019204:GTP-Binding Proteins / Q000235:genetics*; D020962:Heterotrimeric GTP-Binding Proteins; D006801:Humans; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D019204:GTP-Binding Proteins; C112631:G protein alpha 16; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D020962:Heterotrimeric GTP-Binding Proteins