Enhanced accumulation of the isoprostane, 8-epi-PGF2 alpha, in human aortic and pulmonary valves of patients with coronary heart disease.
The aim of the present study was to investigate whether the isoprostane 8-epi-PGF2 alpha differently accumulates in semilunar valves of patients suffering from coronary heart disease (CHD, n = 19) as compared to valves from healthy heart donors (controls, n = 6). Sections from isolated aortic and pulmonary valves were analyzed by semiquantitative immunohistochemistry. The 8-epi-PGF2 alpha-content was determined by using a specific radioimmunoassay. The accumulation of 8-epi-PGF2 alpha in both valves was higher in CHD-patients in comparison to controls (Aortic valves: 36.49 +/- 11.26% vs. 15.78 +/- 3.04%; pulmonary valves: 46.79 +/- 9.80% vs. 14.99 +/- 3.57%). The results from the radioimmunoassay revealed comparable findings in both groups (CHD vs. controls: 395.95 +/- 86.09 vs. 139.50 +/- 47.46 pg/mg protein in the aortic valves and 430.47 +/- 76.30 vs. 147.33 +/- 53.84 pg/mg protein in pulmonary valves). Pulmonary valves seem to be more susceptible to oxidative stress than aortic valves as evidenced by a higher accumulation of 8-epi-PGF2 alpha in CHD patients. Considering the data presented in this study, we suggest that 8-epi-PGF2 alpha is a valuable indicator of oxidative injury in human semilunar valves.
Mehrabi
M R
MR
Department of Cardiology, University of Vienna, Währinger Gürtel 18-20, Postfach 120, A-1097 Vienna, Austria. mohammad.mehrabi@univie.ac.at
Serbecic
N
N
Ekmekcioglu
C
C
Tamaddon
F
F
Wild
T
T
Plesch
K
K
Sinzinger
H
H
Glogar
H D
HD
D000368:Aged; D001021:Aortic Valve / Q000378:metabolism* / Q000473:pathology; D004396:Coloring Agents; D003327:Coronary Disease / Q000150:complications / Q000378:metabolism* / Q000473:pathology*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D028421:Isoprostanes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D011664:Pulmonary Valve / Q000378:metabolism* / Q000473:pathology; D011863:Radioimmunoassay
D004396:Coloring Agents; D028421:Isoprostanes; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost