Screening of tumor necrosis factor-alpha-binding peptides by phage display peptide library.
OBJECTIVE
To identify and characterize tumor necrosis factor (TNF)-alpha-binding peptides from c7c phage display peptide library, in an attempt to find short peptides that can be used as antagonist of TNF-alpha.


METHODS
The TNF-alpha-binding peptides were screened from c7c phage display peptide library by using rhTNF-alpha as target protein and identified by sandwich ELISA.


RESULTS
After 3 rounds of screening, 11 of 23 phage clones were identified as positive clones which can bind to rhTNF-alpha. The amino acid sequence in two of these 11 clones is c-ALWHWWH-c, and that in the others is c-(T/S)WLHWWA-c.


CONCLUSIONS
These phage display peptides are TNFalpha-binding peptides.
Guo
Hai-Ping
HP
Department of Immunology, First Military Medical University, Guangzhou 510515, China.
Luo
Hai-Bo
HB
Liu
Yan-Jun
YJ
Zhu
Ping
P
Fu
Ning
N
D006801:Humans; D007158:Immunologic Techniques; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism*
D019151:Peptide Library; D010455:Peptides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha