Quantitation of yeast ceramides using high-performance liquid chromatography-evaporative light-scattering detection.
A high-performance liquid chromatograph equipped with an evaporative light scattering detector (ELSD) (HPLC-ELSD) was used to assay the ceramides in yeast cells. The HPLC-ELSD method employed a cyanopropyl bonded column (CN column) that effectively separated the main interfering substance ergosterol without any derivatization process; most other interfering substances were also removed. The method can be applied for routine assay of ceramide content in yeast.
Zhou
Quan
Q
Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China.
Zhang
Lin
L
Fu
Xue-Qi
XQ
Chen
Guo-Qiang
GQ
D002518:Ceramides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004875:Ergosterol / Q000032:analysis; D008027:Light; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D012542:Scattering, Radiation
D002518:Ceramides; D004875:Ergosterol