[Effect of angiotensin II on L-type calcium channel in ischemic ventricular myocytes of the guinea pig].
Using whole cell patch clamp, the effects of angiotensin II (Ang II) on the current L-type calcium channel (I(Ca.L)) were observed in guinea pig isolated ventricular myocytes under simulated ischemia condition, which was realized through hypoxia, glucose deficiency, high lactic acid and acidosis. The results showed that, under the condition of simulated ischemia, the peak of I(Ca.L) was reduced with maximal activation potential at 0 mV. Administration of Ang II (100 nmol/L) enhanced the peak of I(Ca.L) during ischemia and shifted the maximal activation potential to -10 mV. The possible mechanism of these effects is discussed.
Wang
Wen-Wei
WW
Department of Physiology, Shanghai Medical College of Fudan University, National Key Laboratory for Medical Neurobiology, Shanghai, China.
Zhu
Yi-Chun
YC
Yao
Tai
T
Zheng
Ping
P
Gong
Qian-Ling
QL
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D020746:Calcium Channels, L-Type / Q000187:drug effects*; D002478:Cells, Cultured; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000166:cytology; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects*; D018408:Patch-Clamp Techniques
D020746:Calcium Channels, L-Type; D000804:Angiotensin II