title: Use of a new computerized system for evaluation of spermatozoal motility and velocity characteristics in relation to fertility levels in dromedary bulls.
abstract: Three ejaculates from each of 14 dromedary bulls were collected at 7-day intervals and diluted to 50 x 10 (6) spermatozoa per ml with sodium citrate (2.9%) seminal extender. Spermatozoal concentrations, motility percentages and velocity measures were evaluated by a new computerized cell motion analyzer (CMA, medical Technologies Montreux SA, Switzerland) for assessing fertility rates in such animals. Greatest variability in concentrations and motility percentages was generally attributed to animal effect within the first ejaculate. Spermatozoal concentrations and kinematic variables in particular percentages of progressive motility, amplitude of lateral head displacement (ALH) and linearity (LIN) percentage were strongly correlated (P<0.01) with fertility rates of dromedary bulls. The CMA-derived measurements of velocity straight line (VSL), velocity curve line (VCL), and velocity averaged line (VAP) were significantly affected by the type of spermatozoal tracks. The present results evidenced that the CMA is a reliable system for determining spermatozoal concentrations, motility percentages and velocity measures and is considered as an accurate and rapid method for evaluating and predicting fertility in the one-humped camel bulls.
authors.affiliation: Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, King Saud University, PO Box 1482, Bureidah, Al-Qassim, Saudi Arabia.
authors.forename: A A
authors.initials: AA
authors.lastname: Al-Qarawi
authors.forename: H A
authors.initials: HA
authors.lastname: Abdel-Rahman
authors.forename: S A
authors.initials: SA
authors.lastname: El-Mougy
authors.forename: M S
authors.initials: MS
authors.lastname: El-Belely
mesh_terms: D000818:Animals; D002162:Camelus / Q000502:physiology*; D004542:Ejaculation; D005260:Female; D005298:Fertility / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D008297:Male; D008858:Microscopy, Phase-Contrast / Q000662:veterinary; D015999:Multivariate Analysis; D011247:Pregnancy; D012661:Semen / Q000502:physiology; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology