title: The use of cytoplasmic markers in onion hybrid breeding.
abstract: We applied the RFLP approach to identify the cytoplasmic genotypes of selected onion breeding materials from Poland. For this purpose, mitochondrial DNA from cytoplasmic male-sterile (CMS) and male-fertile onions were hybridized with the probes for the following mitochondrial genes: atpA, atp6, atp9, cob, cox1, nad3, nad4 and nad6. S-, T- or C-cytoplasm was represented in each analyzed sterile accession. Some new polymorphisms shared by S- and C-cytoplasmic onions were identified. We also used currently available PCR markers to test if cytoplasmic heterogenity occurs within onion inbreds. A fraction of the plants bearing S-cytoplasm were found within two male-fertile lines, but such plants were not detected in the open-pollinated cultivars Sochaczewska, Wolska and Zytawska. Both the RFLP and PCR approaches gave some proof of existing mitochondrial heteroplasmy in onions
authors.affiliation: Department of Genetics, Plant Breeding and Seed Science, Agricultural University of Krakó, 29 Listopada 54, 31-425 Kraków, Poland. marekszk@hotmail.com
authors.forename: Marek
authors.initials: M
authors.lastname: Szklarczyk
authors.forename: Magdalena
authors.initials: M
authors.lastname: Simlat
authors.forename: Barbara
authors.initials: B
authors.lastname: Jagosz
authors.forename: Grazyna
authors.initials: G
authors.lastname: Ba
mesh_terms: D001947:Breeding; D003593:Cytoplasm / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D006824:Hybridization, Genetic; D019697:Onions / Q000235:genetics*; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004272:DNA, Mitochondrial; D018744:DNA, Plant; D005819:Genetic Markers