KClO(4) inhibits thyroidal activity in the larval lamprey endostyle in vitro.
An in vitro experimental system was devised to assess the direct effects of the goitrogen, potassium perchlorate (KClO(4)), on radioiodide uptake and organification by the larval lamprey endostyle. Organification refers to the incorporation of iodide into lamprey thyroglobulin (Tg). Histological and biochemical evidence indicated that endostyles were viable at the termination of a 4h in vitro incubation. A single iodoprotein, designated as lamprey Tg, was identified in the endostylar homogenates by polyacrylamide gel electrophoresis and Western blotting. Lamprey Tg was immunoreactive with rabbit anti-human Tg serum and had an electrophoretic mobility similar to that of reduced porcine Tg. When KClO(4) was added to the incubation medium, both iodide uptake and organification by the endostyle were significantly reduced relative to controls as determined by gamma counting, and gel-autoradiography and densitometry, respectively. Western blotting showed that KClO(4) significantly lowered the total amount of lamprey Tg in the endostyle. Based on the results of this in vitro investigation, we conclude that KClO(4) acts directly on the larval lamprey endostyle to inhibit thyroidal activity. These data support a previous supposition from in vivo experimentation that KClO(4) acts directly on the endostyle to suppress the synthesis of thyroxine and triiodothyronine, resulting in a decrease in the serum levels of these two hormones.
Manzon
Richard G
RG
Department of Zoology and Division of Life Sciences, University of Toronto at Scarborough, 1265 Military Trail, Toronto, Ont, Canada MIC 1A4. Richard.Manzon@uregina.ca
Youson
John H
JH
D000704:Analysis of Variance; D000818:Animals; D013956:Antithyroid Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004707:Endoderm / Q000187:drug effects / Q000473:pathology; D066298:In Vitro Techniques; D007455:Iodine / Q000493:pharmacokinetics; D007798:Lampreys / Q000254:growth & development*; D007814:Larva / Q000187:drug effects; D008675:Metamorphosis, Biological / Q000187:drug effects; D009024:Morphogenesis / Q000187:drug effects; D010472:Perchlorates / Q000494:pharmacology*; D017680:Potassium Compounds / Q000494:pharmacology*; D013954:Thyroglobulin / Q000032:analysis / Q000187:drug effects*; D013961:Thyroid Gland / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D013963:Thyroid Hormones / Q000378:metabolism
D013956:Antithyroid Agents; D010472:Perchlorates; D017680:Potassium Compounds; D013963:Thyroid Hormones; C009006:potassium perchlorate; D013954:Thyroglobulin; D007455:Iodine