RNAi and viral vectors as useful tools in the functional genomics of plants. Construction of BMV-based vectors for RNA delivery into plant cells.
The sequencing of several complete genomes and the development of a DNA microarray technology are among the most important achievements of molecular biology. They gave the proper grounds for the development of modern functional genomics. However, there is one additional condition which needs to be satisfied to truely enable the study of how a genome works: a suitable method of selectively inducing and silencing the expression of each individual gene. The methods used so far have usually only permitted the influencing of gene expression through genetic manipulations at the DNA level (genetically modified plants). The discovery of RNA interference (RNAi) opens up completely new possibilities of research on the functioning of particular plant genes, without the necessity of altering the genome structure. In this case, interference takes place at the transcript level. Thus, at any given moment during plant development, the expression of a specific gene (or several genes) can be inhibited, even if it is important for the survival of the organism under study. To this end, a double-stranded RNA inducing the RNAi phenomenon has to be delivered into the plant cell. Here we describe the construction of four brome mosaic virus-based vectors, which, as our preliminary data indicate, can be used to transfer RNA into barley cells.
Wojtkowiak
Agnieszka
A
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland.
Siek
Anna
A
Alejska
Magdalena
M
Jarmołowski
Artur
A
Szweykowska-Kulińska
Zofia
Z
Figlerowicz
Marek
M
D017795:Bromovirus / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017343:Genes, Plant; D005822:Genetic Vectors / Q000235:genetics*; D018745:Genome, Plant; D001467:Hordeum / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D012313:RNA / Q000008:administration & dosage*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014170:Transformation, Genetic
D010940:Plant Proteins; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D018749:RNA, Plant; D034741:RNA, Small Interfering; C039919:RNA, recombinant; D011993:Recombinant Fusion Proteins; D012313:RNA