Inositol pyrophosphates regulate endocytic trafficking.
The high energy potential and rapid turnover of the recently discovered inositol pyrophosphates, such as diphosphoinositol-pentakisphosphate and bis-diphosphoinositol-tetrakisphosphate, suggest a dynamic cellular role, but no specific functions have yet been established. Using several yeast mutants with defects in inositol phosphate metabolism, we identify dramatic membrane defects selectively associated with deficient formation of inositol pyrophosphates. We show that this phenotype reflects specific abnormalities in endocytic pathways and not other components of membrane trafficking. Thus, inositol pyrophosphates are major regulators of endocytosis.
Saiardi
Adolfo
A
Department of Neuroscience, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.
Sciambi
Catherine
C
McCaffery
J Michael
JM
Wendland
Beverly
B
Snyder
Solomon H
SH
D004705:Endocytosis; D007295:Inositol Phosphates / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
D007295:Inositol Phosphates; D029701:Saccharomyces cerevisiae Proteins; C079849:1-diphosphoinositol pentakisphosphate; C079848:bis(1,4)-diphosphoinositol tetrakisphosphate; C066454:inositol 4,5-bisphosphate; C120127:IPK1 protein, S cerevisiae; D017853:Phosphotransferases (Alcohol Group Acceptor); C434257:inositol polyphosphate multikinase; D017856:Phosphotransferases (Phosphate Group Acceptor); C099438:inositol hexakisphosphate kinase