Liquid chromatographic determination of florfenicol in the plasma of multiple species of fish.
A simple method was developed for determining florfenicol concentration in a small volume (250 micro l) of plasma from five phylogenetically diverse species of freshwater fish. Florfenicol was isolated from the plasma matrix through C(18) solid-phase extraction and quantified by reversed-phase high-performance liquid chromatography with UV detection. The accuracy (84-104%), precision (%RSD<or=8), and sensitivity (quantitation limit <30 ng/ml) of the method indicate its usefulness for conducting pharmacokinetic studies on a variety of freshwater fish.
Vue
Chue
C
US Geological Survey, Upper Midwest Environmental Sciences Center, 2630 Fanta Reed Road, La Crosse, WI 54603, USA. chue_vue@usgs.gov
Schmidt
Larry J
LJ
Stehly
Guy R
GR
Gingerich
William H
WH
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005399:Fishes; D015203:Reproducibility of Results; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics
C035534:florfenicol; D013839:Thiamphenicol