[Agroinfiltration, a useful technique in plant molecular biology research].
Agroinfiltration is a newly developed plant transient gene expression technique, which is simple, rapid and reproducible. It has been widely used in analyses of foreign gene expression, hypersensitive reaction, gene silencing, promoter activity and identification of new disease-resistance genes. In this paper, we describe the principle and the operation procedure of Agroinfiltration and its application in diverse aspects of plant molecular biology research. Our experiences in modification of the Agroinfiltration technique are also provided.
Liu
Zhao-Ming
ZM
Institute of Microbiology, Chinese Academy of Science, Beijing 100080, China.
Liu
Zong-Zhi
ZZ
Bai
Qing-Wu
QW
Fang
Rong-Xiang
RX
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D012107:Research Design