Cytokine production by the murine macrophage cell line J774.1 after exposure to lactobacilli.
Eleven strains of lactobacilli were tested for their ability to induce the murine macrophage-like cell line J774.1 to secrete cytokines. Some of the bacteria tested induce the production of interleukin(IL) 6, IL-12, and tumor necrosis factor a (TNF-alpha) by J774.1 cells. Seven strains also induced the production of IL-10. However, no IL-1beta was produced. Lactobacillus acidophilus TMC 0356 significantly induced the production of more IL-6, IL-10, IL-12, and TNF-alpha than the other bacteria tested (p < 0.0001; ANOVA). These results suggest that lactobacilli can activate macrophages to secrete both inflammatory and anti-inflammatory cytokines. Selected strains might be used to bring about pro or antiinflammatory immune reactions.
Morita
Hirotsugu
H
Technical Research Laboratory, Takanashi Milk Products Co, Ltd, Yokohama, Kanagawa, Japan.
He
Fang
F
Fuse
Tetsuo
T
Ouwehand
Arthur C
AC
Hashimoto
Hideo
H
Hosoda
Masataka
M
Mizumachi
Koko
K
Kurisaki
Jun-Ichi
J
D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D007778:Lactobacillus / Q000502:physiology*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D051379:Mice
D016207:Cytokines