title: Isolation and characterisation of nuclear mutants with enhanced mitochondrial mutability in the fission yeast Schizosaccharomyces pombe.
abstract: In this paper we report the isolation and preliminary characterisation of nuclear mutants with increased mitochondrial mutability in fission yeast. Screening of about 2000 clones after nitrosoguanidine mutagenesis led to the isolation of ten mutator mutants. For one of them (mut-1) we show that the mutation is chromosomally encoded. The activity of the mutator is restricted to the mitochondrial genome, since it increases the mutation rate to mitochondrially encoded drug resistance considerably, whereas the mutability of nuclear genes is not altered.
authors.affiliation: International Institute of Genetics and Biophysics, CNR, Naples, Italy.
authors.forename: Domenica Rita
authors.initials: DR
authors.lastname: Massardo
authors.forename: Luigi
authors.initials: L
authors.lastname: Del Giudice
authors.forename: Angelica
authors.initials: A
authors.lastname: Del Giudice
authors.forename: Klaus
authors.initials: K
authors.lastname: Wolf
mesh_terms: D002467:Cell Nucleus / Q000235:genetics*; D003573:Cytochrome b Group / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics*; D004237:Diuron / Q000378:metabolism / Q000494:pharmacology; D025141:Drug Resistance, Fungal; D009154:Mutation; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D003573:Cytochrome b Group; D004272:DNA, Mitochondrial; D004237:Diuron