title: Maternal lymphocyte subpopulations (CD45RA+ and CD45RO+) in preeclampsia.
abstract: OBJECTIVE
  The maternal syndrome of preeclampsia has been attributed to a systemic intravascular inflammatory response and endothelial cell dysfunction. The stimulus responsible for intravascular inflammation in preeclampsia has not been determined. The expression of CD45 isoforms on the surface of human T cells has been used to classify CD4(+) T lymphocytes into naïve cells (CD45RA+) and memory T cells (CD45RO+). An increased percentage of CD45RO+ cells has been interpreted as consistent with previous exposure to microbial products or other antigens. The purpose of this study was to determine whether preeclampsia is associated with a change in the proportion of CD45RA+ and CD45RO+.
  
  
  METHODS
  A prospective study was conducted in patients with preeclampsia (n = 24) and normal pregnancy (n = 75). The percentage of CD45RA+ and CD45RO+ on CD4(+) T lymphocytes in peripheral blood was determined using flow cytometry and monoclonal antibodies. Results were reported as a percentage of CD4(+) lymphocytes. Parametric statistics were used for analysis. A probability value of <.05 was considered statistically significant.
  
  
  RESULTS
  Patients with preeclampsia had a significantly higher percentage of CD45RO+ than normal pregnant women (P <.01). A significantly lower percentage of CD45RA+ was found in patients with preeclampsia than in normal pregnant women (P <.01).
  
  
  CONCLUSIONS
  Preeclampsia is associated with an increase in the percentage of CD45RO+ and a decrease in the CD45RA+ lymphocyte subpopulation. Therefore, patients with preeclampsia have evidence of previous antigenic exposure, the nature of which remains to be established.
authors.affiliation: Perinatology Research Branch, National Institute of Child Health and Human Development, and the Department of Obstetrics and Gynecology, Wayne State University/Hutzel Hospital, 4707 St. Antoine Boulevard, Detroit, MI 48201, USA.
authors.forename: Tinnakorn
authors.initials: T
authors.lastname: Chaiworapongsa
authors.forename: Maria-Teresa
authors.initials: MT
authors.lastname: Gervasi
authors.forename: Jerrie
authors.initials: J
authors.lastname: Refuerzo
authors.forename: Jimmy
authors.initials: J
authors.lastname: Espinoza
authors.forename: Jun
authors.initials: J
authors.lastname: Yoshimatsu
authors.forename: Susan
authors.initials: S
authors.lastname: Berman
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D003430:Cross-Sectional Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis*; D011225:Pre-Eclampsia / Q000276:immunology*; D011247:Pregnancy; D011446:Prospective Studies; D012016:Reference Values
chemical_list: D000911:Antibodies, Monoclonal; D017493:Leukocyte Common Antigens