Effect of hypothyroidism on synaptosomal-associated protein of 25 kDa and syntaxin-1 expression in adenohypophyses of rat.
The aim of this study was to analyze the expression of SNAP-25 and syntaxin-1 in the adenohypophyses of hypothyroid rats. Rats were divided into: 1) controls; 2) thyroidectomized 40 days; and 3) thyroidectomized 40 days with replacement of T4 20 days after surgery. Adenohypophyses were studied by immunohistochemistry and immunoblot analysis using antibodies against SNAP-25, syntaxin-1 and TSH. By immunostaining, SNAP-25 and syntaxin-1 were conspicuous and were localized around cytoplasmic vacuoles of thyroidectomy cells. Immunoblot analysis shows that thyroidectomy increases adenohypophysial SNAP-25 expression and decreases syntaxin-1 levels. T4 administration for 20 days produces a recovery similar to control values. In conclusion, thyroidectomy produces changes in both expression and immunoreactivity of SNAP-25 and syntaxin-1 in adenohypophyses of rats and these effects can be reversed by T4 administration.
Quintanar
J L
JL
Department of Physiology and Pharmacology, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, México. jlquinta@correo.uaa.mx
Salinas
E
E
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D005260:Female; D007037:Hypothyroidism / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D010903:Pituitary Gland, Anterior / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1; D013965:Thyroidectomy; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood / Q000494:pharmacology
D000954:Antigens, Surface; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C494210:Snap25 protein, rat; C494213:Stx1a protein, rat; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1; D013972:Thyrotropin; D013974:Thyroxine