Influence of severe endometriosis on gene expression of vascular endothelial growth factor and interleukin-6 in granulosa cells from patients undergoing controlled ovarian hyperstimulation for in vitro fertilization-embryo transfer.
OBJECTIVE
To evaluate how endometriosis affects expression of vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) in granulosa cells.


METHODS
Prospective study.


METHODS
IVF-ET program at Osaka Medical College.


METHODS
Seventeen patients with revised American Fertility Society stage IV endometriosis and 17 patients with tubal infertility and no endometriosis.


METHODS
Granulosa cells obtained at oocyte retrieval were examined for VEGF and IL-6 gene expression.


METHODS
Serum E(2) and P levels at hCG administration, number of oocytes, fertilization rate, high-quality embryo rate, and pregnancy rate, and expression of VEGF and IL-6 genes.


RESULTS
Total hMG and FSH levels were statistically significantly higher in patients with endometriosis; however, the number of retrieved oocytes and the fertilization rate were lower compared with patients with tubal infertility. Serum E(2) levels and expression of VEGF in patients with tubal infertility were statistically significantly higher than those in patients with endometriosis. Interleukin-6 gene expression did not differ between the groups.


CONCLUSIONS
In severe endometriosis, lower VEGF gene expression in granulosa cells may adversely affect oocyte development and maturation.
Yamashita
Yoshiki
Y
Department of Obstetrics and Gynecology, Osaka Medical College, Takatsuki, Osaka, Japan. gyn028@poh.osaka-med.ac.jp
Ueda
Masatsugu
M
Takehara
Mikio
M
Yamashita
Hikari
H
Suzuki
Yoshihiko
Y
Hung
Yao-Ching
YC
Terai
Yoshito
Y
Ueki
Minoru
M
D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D004624:Embryo Transfer; D004715:Endometriosis / Q000150:complications / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000235:genetics*; D004958:Estradiol / Q000097:blood; D005184:Fallopian Tube Diseases / Q000150:complications; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000097:blood; D015870:Gene Expression; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000628:therapy; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D008222:Lymphokines / Q000235:genetics*; D008596:Menotropins / Q000097:blood; D009865:Oocytes / Q000502:physiology; D010062:Ovulation Induction; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011446:Prospective Studies; D027724:Reproductive Techniques, Assisted; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D006063:Chorionic Gonadotropin; D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D015850:Interleukin-6; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D011374:Progesterone; D004958:Estradiol; D008596:Menotropins; D005640:Follicle Stimulating Hormone