Changes of sialomolecules during the dimethylsulfoxide-induced differentiation of Herpetomonas samuelpessoai.
The expression of sialoglycoconjugates during the dimethylsulfoxide (DMSO)-induced differentiation of Herpetomonas samuelpessoai was analyzed by flow cytometry and Western blotting using sialic acid-specific lectins. Parasites reacted strongly with Limax flavus (LFA) and Sambucus nigra (SNA) agglutinins, and only weakly with Maackia amurensis (MAA) lectin. However, analysis of crude protein extracts by Western blotting revealed that bands with molecular masses corresponding to 15 and 40 kDa are recognized by MAA, and that treatment with DMSO induced the expression of two additional polypeptides with molecular masses of 65 and 90 kDa. Profiles of binding to LFA were indistinguishable when protein extracts from control or differentiated cells were analyzed. SNA recognized a major molecule with 25 kDa in extracts from non-differentiated forms and two low-molecular-weight bands from differentiated cells. These results indicate that molecules containing alpha2,6 and alpha2,3 sialyl-galactosyl sequences are present in H. samuelpessoai, and that their biosynthesis and expression are influenced by DMSO-induced differentiation.
dos Santos
André Luis Souza
AL
Departamento de Microbiologia Geral, Instituto de Microbiologia Prof Paulo de Góes, Centro de Ciências da Saúde, Bloco I, Universidade do Brasil (UFRJ), Ilha do Fundão, 21941-590, Rio de Janeiro, RJ, Brazil.
Rodrigues
Márcio Lourenço
ML
Alviano
Celuta Sales
CS
de Araujo Soares
Rosangela Maria
RM
D000818:Animals; D015153:Blotting, Western; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006001:Glycoconjugates / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism; D012794:Sialic Acids / Q000378:metabolism*; D014351:Trypanosomatina / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development*
D006001:Glycoconjugates; D015800:Protozoan Proteins; D012794:Sialic Acids; D004121:Dimethyl Sulfoxide