Vesicular stomatitis virus nucleocapsid protein production in cells treated with selected fast protein liquid chromatography fractions of tick salivary gland extracts.
A salivary gland extract (SGE) prepared from 5-days-fed Dermacentor reticulatus female ticks was fractionated by fast protein liquid chromatography (FPLC). The effect of three FPLC fractions selected on the basis of anti-interleukin 8 (anti-IL-8) activity on vesicular stomatitis virus (VSV) nucleocapsid (N) protein formation in mouse L-cells was determined. Infected 14C-labeled cells treated with the FPLC fractions were analyzed by two-dimensional (2D) electrophoresis. The yields of VSV N protein were evaluated by Imagemaster software analysis. Most noticeable was an increase in the N protein production after treatment with the fraction 39 corresponding to the major peak of the anti-IL-8 activity. The nature of the substance in SGE that was responsible for this effect remains unclear.
Sláviková
M
M
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Kocáková
P
P
Slovák
M
M
Vancová
I
I
Hajnická
V
V
Gasperík
J
J
Fuchsberger
N
N
Nuttall
P A
PA
D000818:Animals; D002457:Cell Extracts / Q000494:pharmacology; D002458:Cell Fractionation; D002853:Chromatography, Liquid; D003870:Dermacentor / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D016209:Interleukin-8 / Q000302:isolation & purification / Q000378:metabolism; D007739:L Cells; D051379:Mice; D019251:Nucleocapsid / Q000096:biosynthesis* / Q000378:metabolism; D019590:Nucleocapsid Proteins; D012469:Salivary Glands / Q000737:chemistry* / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism*
D002457:Cell Extracts; D016209:Interleukin-8; D019590:Nucleocapsid Proteins