title: Phage-displayed mimotopes recognizing a biologically active anti-HIV-1 gp120 murine monoclonal antibody.
abstract: Antibody-dependent cellular cytotoxicity (ADCC) is a host defense mechanism in which Fc receptor-bearing effector cells in combination with antigen-specific antibodies recognize and kill antigen-expressing target cells. The authors previously described a murine monoclonal antibody (MAb-ID6) that mediated ADCC activity against HIV-infected cells. It was demonstrated that the specificity of MAb-ID6 maps to the first 204 amino acids of gp120; however, the exact epitope was not identified. In the present work, by screening phage display libraries with MAb-ID6, the authors have mapped the corresponding epitope to amino acids 86-100 (HIV-1 gp120 sequence). This epitope lies within the C1 region of gp120 and is highly conserved among all subtypes and circulating recombinant forms of HIV-1. Thus, these phage mimotopes of C1 may serve as components of a vaccine for the induction of gp120-specific antibodies mimicking MAb-ID6.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, DF.
authors.forename: Victor Raul
authors.initials: VR
authors.lastname: Gómez-Román
authors.forename: Chuanhai
authors.initials: C
authors.lastname: Cao
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
authors.forename: Hugo
authors.initials: H
authors.lastname: Santamaría
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Acero
authors.forename: Karen
authors.initials: K
authors.lastname: Manoutcharian
authors.forename: David B
authors.initials: DB
authors.lastname: Weiner
authors.forename: Kenneth E
authors.initials: KE
authors.lastname: Ugen
authors.forename: Goar
authors.initials: G
authors.lastname: Gevorkian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology; D001435:Bacteriophages / Q000737:chemistry / Q000276:immunology*; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D015483:HIV Antibodies / Q000096:biosynthesis / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D051379:Mice; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D019151:Peptide Library
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D019151:Peptide Library