title: Secretory phospholipase A2 and phospholipids in neural membranes in an experimental epilepsy model.
abstract: OBJECTIVE
  Previous studies have revealed an increase in several forms of phospholipase A2 activity associated with cell injury, but the secretory form of phospholipase A2 has not previously been studied in neurological disorders. We investigated the influence of seizures on secretory phospholipase A2 and phospholipid breakdown in synaptosome fractions prepared from rat hippocampus, cortex and cerebellum in pentylenetetrazol-induced epilepsy.
  
  
  METHODS
  Secretory phospholipase A2 concentration was measured by a photometric enzyme immunoassay. The synaptosomes underwent extraction, and the phospholipids fractions for phosphatidylcholine, phosphatidylethanolamine and lysophosphatidylcholine were recovered from the thin layer chromatography plates. The amount of each phospholipid was quantified using the amount of recovered phosphate in each phospholipid spot.
  
  
  RESULTS
  Secretory phospholipase A2 concentration was found to be significantly higher in the epileptic group when compared with the control group. The amounts of phospholipids were found to be highly variable in different brain regions.
  
  
  CONCLUSIONS
  Our results suggest that epileptic seizures enhanced phospholipid breakdown and induced alterations in the distribution of phospholipids in different brain regions.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Yegin
authors.forename: S H
authors.initials: SH
authors.lastname: Akbas
authors.forename: T
authors.initials: T
authors.lastname: Ozben
authors.forename: D K
authors.initials: DK
authors.lastname: Korgun
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000201:enzymology* / Q000378:metabolism; D002531:Cerebellum / Q000201:enzymology* / Q000378:metabolism; D002540:Cerebral Cortex / Q000201:enzymology* / Q000378:metabolism; D003292:Convulsants / Q000009:adverse effects; D004195:Disease Models, Animal; D004827:Epilepsy / Q000139:chemically induced / Q000201:enzymology*; D005260:Female; D006624:Hippocampus / Q000201:enzymology* / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000032:analysis; D009474:Neurons / Q000201:enzymology* / Q000378:metabolism; D010433:Pentylenetetrazole / Q000009:adverse effects; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010741:Phospholipases A / Q000032:analysis* / Q000378:metabolism*; D054467:Phospholipases A2; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000201:enzymology* / Q000378:metabolism
chemical_list: D003292:Convulsants; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010741:Phospholipases A; D054467:Phospholipases A2; D010433:Pentylenetetrazole