Differential transcriptional expression of Ca2+ BP superfamilies in murine gastrointestinal smooth muscles.
Calmodulin (Cal) plays important roles for contractile activity in smooth muscles. Recently, two distinct Ca(2+)-binding protein superfamilies with sequence similarities to Cal have been identified in neuronal cells: neuronal Ca(2+)-binding proteins (NCBPs) and Cal-like Ca(2+)-binding proteins (CaBPs). Some NCBPs and CaBPs play significant roles for Ca(2+)-dependent cellular signaling in the nervous system. In gastrointestinal smooth muscles (GISMs), Cal functions as the regulator of contractile behavior and electrical rhythmicity. However, the molecular identification of NCBPs and CaBPs has not been elucidated in GISMs. Here, we have identified NCBPs and CaBPs expressed in GISMs and determined the expression levels of their transcripts by quantitative RT-PCR. Of 12 NCBPs, the transcripts for neuronal Ca(2+) sensor 1, neural visinin-like proteins 1, 2, and 3, and K(+) channel-interacting proteins 1 and 3 were detected in proximal colon, gastric fundus, gastric antrum, and jejunum. On the other hand, of seven CaBPs including alternatively spliced variants, only CaBP1L transcripts were detected in GISMs.
Ohya
Susumu
S
Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada 89557-0046, USA.
Horowitz
Burton
B
D017398:Alternative Splicing; D000818:Animals; D001923:Brain Chemistry; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000502:physiology; D002147:Calmodulin / Q000502:physiology; D003106:Colon / Q000737:chemistry; D004064:Digestive System / Q000737:chemistry / Q000378:metabolism*; D005748:Gastric Fundus / Q000737:chemistry; D015870:Gene Expression; D007583:Jejunum / Q000737:chemistry; D051605:Kv Channel-Interacting Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000737:chemistry / Q000378:metabolism*; D009206:Myocardium / Q000737:chemistry; D009419:Nerve Tissue Proteins / Q000235:genetics; D051596:Neurocalcin; D051580:Neuronal Calcium-Sensor Proteins; D009474:Neurons / Q000737:chemistry; D009479:Neuropeptides / Q000235:genetics; D011706:Pyloric Antrum / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D044169:Receptors, Calcium-Sensing; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D002135:Calcium-Binding Proteins; D002147:Calmodulin; C496756:Csen protein, mouse; C496754:Kcnip1 protein, mouse; D051605:Kv Channel-Interacting Proteins; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; D012333:RNA, Messenger; D044169:Receptors, Calcium-Sensing; D012097:Repressor Proteins; C496689:Vsnl1 protein, mouse; C101464:frequenin calcium sensor proteins