Functional expression of Acetabularia acetabulum vacuolar H(+)-pyrophosphatase in a yeast VMA3-deficient strain.
The function of the translation product of cDNA for Acetabularia vacuolar H(+)-pyrophosphatase was examined using the Saccharomyces cerevisiae VMA3-deficient strain. The open reading frame of Acetabularia H(+)-pyrophosphatase was revealed to encode 751 amino acids (721 or 751 amino acids in a previous paper). The acidification of the vacuole was observed by fluorescence microscopy when the cDNA was constructed in pYES2. Immunoblot analysis also supported the localization of the translation product in the vacuolar-membrane-enriched fraction.
Ikeda
Mikiko
M
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Soja 719-1197, Japan. mikeda@fhw.oka-pu.ac.jp
Umami
Kimiko
K
Hinohara
Misato
M
Tanimura
Yoshie
Y
Ohmae
Atsuko
A
Nakanishi
Yoichi
Y
Maeshima
Masayoshi
M
D000076:Acetabularia / Q000201:enzymology* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D006863:Hydrogen-Ion Concentration; D043564:Inorganic Pyrophosphatase; D008856:Microscopy, Fluorescence; D009154:Mutation; D011755:Pyrophosphatases / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014617:Vacuoles / Q000201:enzymology* / Q000235:genetics
D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase