Molecular cytogenetic analysis of the monoblastic cell line U937. karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization.
Previous reports on the analysis of the human monoblastic cell line U937 had described several sublines containing unidentified rearrangements and marker chromosomes. In order to determine the true nature of the rearrangements, conventional banding analysis was carried out with various combinations of molecular cytogenetic techniques: comparative genomic hybridization, fluorescence in situ hybridization (FISH) with whole chromosome painting probes, and microdissection and reverse painting FISH. The origins of the marker chromosomes were identified and the composite karyotype is described.
Lee
Ji-Yun
JY
Department of Medical Genetics, College of Medicine, Hanyang University, Seoul 133-791, South Korea.
Lee
Chul-Hoon
CH
Shim
Sung-Han
SH
Seo
Han-Kyu
HK
Kyhm
Jee-Hong
JH
Cho
Sechin
S
Cho
Youl-Hee
YH
D002871:Chromosome Banding / Q000379:methods*; D020223:Chromosome Painting / Q000379:methods*; D002877:Chromosomes, Human / Q000235:genetics; D004210:Dissection; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020298:U937 Cells