[Investigation of the gene expression profile in spermatocytes].
OBJECTIVE
To investigate the gene expression profile in the mature spermatocytes and the molecular mechanism of the male infertility syndromes.


METHODS
Restriction display PCR (RD-PCR), a new method of differential display technique, was used to collect plenty of gene fragments expressed in either normal or abnormal spermatocytes, the gene expression profile of which were demonstrated by these fragments(gene expression sequence tags, ESTs), in the form of cDNA fragments length polymorphism. These ESTs will be used to make DNA microarray as probes to further explore the gene expression profile in spermatocytes and to diagnose the male infertility.


RESULTS
Lots of ESTs were expressed in either normal or abnormal spermatocytes and some differentially expressed ESTs have been collected and purified.


CONCLUSIONS
Either for known or unknown genes, RD-PCR is very effective in rapid acquisition of large amount of ESTs with proper and similar length fit for making microarray as the probes.
Mao
Xiang-Ming
XM
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China. mxm@fimmu.edu.cn
Ma
Wen-Li
WL
Peng
Yi-Fei
YF
Zheng
Wen-Ling
WL
D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D012150:Polymorphism, Restriction Fragment Length; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013090:Spermatocytes / Q000378:metabolism*
D012313:RNA