title: Cloning and expression of the nucleocapsid protein gene of nipah virus in different strains of Escherichia coli.
abstract: The coding region of the nucleocapsid (N) gene was amplified from the viral RNA and inserted into the bacterial expression vector, pTrcHis2, for intracellular expression in three Escherichia coli strains: TOP 10, BL 21 and SG 935. The N protein was expressed as a fusion protein containing the myc epitope and His-tag at its C-terminal end. The amount of the fusion protein expressed in strain SG 935 was significantly higher than the other two strains, and was detected by the anti-myc antibody, anti-His and swine anti-NiV serum. Hence, the N(fus) protein produced in E. coli could serve as an alternative antigen for the detection of anti-NiV in swine.
authors.affiliation: Department of Biochemistry and Microbiology, Faculty of Science and Environmental Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
authors.forename: Swee Tin
authors.initials: ST
authors.lastname: Ong
authors.forename: Wen Siang
authors.initials: WS
authors.lastname: Tan
authors.forename: Sharifah Syed
authors.initials: SS
authors.lastname: Hassan
authors.forename: Mohd Azmi
authors.initials: MA
authors.lastname: Mohd Lila
authors.forename: Khatijah
authors.initials: K
authors.lastname: Yusoff
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D019590:Nucleocapsid Proteins / Q000235:genetics* / Q000302:isolation & purification; D018098:Paramyxovirinae / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D011994:Recombinant Proteins; D015678:Viral Structural Proteins