Human meprin beta: O-linked glycans in the intervening region of the type I membrane protein protect the C-terminal region from proteolytic cleavage and diminish its secretion.
Human meprin (hmeprin; N -benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase; EC 3.4.24.18) is a member of the astacin family of zinc metalloendopeptidases. The major site of expression is the brush border membrane of small intestinal and kidney epithelial cells. The enzyme is a type I integral membrane protein composed of two distinct subunits, alpha and beta, which are linked by disulphide bridges. The enzyme complex is attached to the plasma membrane only via the beta-subunit. The alpha-subunit is cleaved in the endoplasmic reticulum in a constitutive manner to remove the C-terminal membrane anchor which leads to secretion of the protein. While the beta-subunit of hmeprin remains largely attached to the brush-border membrane some proteolytic processing occurs intracellularly as well as at the cell surface and results in the release of this subunit from the cell. In the present paper, we report that the beta-subunit bears multiple O-linked sugar residues in the intervening domain. In contrast, the alpha-subunit does not contain O-linked oligosaccharides. Our results show that the O-linked carbohydrate side chains in hmeprinbeta are clustered around a 13 amino acid sequence that contains the main cleavage site for proteolytic processing of the subunit. Prevention of O-glycosylation by specific inhibitors leads to enhanced proteolytic processing and the consequence is an increased release of hmeprinbeta into the culture medium.
Leuenberger
Boris
B
Institute of Biochemistry and Molecular Biology and Department of Pediatrics, Faculty of Medicine, University of Berne, Switzerland.
Hahn
Dagmar
D
Pischitzis
Anastassios
A
Hansen
Marianne K
MK
Sterchi
Erwin E
EE
D000116:Acetylgalactosamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001593:Benzyl Compounds / Q000494:pharmacology; D002236:Carbohydrate Conformation; D002364:Caseins / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D021122:Protein Subunits; D034622:RNA Interference
D001593:Benzyl Compounds; D002364:Caseins; D008565:Membrane Proteins; D011134:Polysaccharides; D021122:Protein Subunits; C026018:azocasein; C080739:benzyl-alpha-N-acetylgalactosamine; D008666:Metalloendopeptidases; C053833:meprin A; C095663:meprin B; D000116:Acetylgalactosamine