Constitutive overexpression of immunoidentical forms of PCP-induced Euglena gracilis CYP-450.
Environmental pollutants are classically associated with increased drug metabolism. In this report, antibodies that are able to detect mammalian CYP proteins, namely the CYP1A1, CYP1A2, CYP2B1/B2, and CYP3A4 proteins, were used to investigate the expression of CYP-related proteins in Euglena gracilis (EG) cells under normal and PCP-treated conditions and in a EG-cell line adapted to PCP. Compared to normal conditions, the presence of PCP in the culture medium induced elevated levels of EG CYP-like proteins. With the exception of CYP3A4, this overexpression was correlated with expression of additional forms of CYP proteins having, respectively, the same molecular weight but slightly different pIs. Even in EG cells which had lost their PCP-adapted property after having been cultured without PCP, these additional forms were continuously expressed. This observation raised the question about the definition of a biomarker of pollution.
Barque
Jean-Philippe
JP
Faculté de Pharmacie, Laboratoire de Biologie Cellulaire et Biochimie, EA 1595, rue J.B. Clément, Châtenay-Malabry Cedex 92296, France.
Abahamid
Abdelouahab
A
Flinois
Jean-Pierre
JP
Beaune
Philippe
P
Bonaly
Jacqueline
J
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002460:Cell Line; D003429:Cross Reactions; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000276:immunology*; D004785:Environmental Pollutants / Q000494:pharmacology*; D005056:Euglena gracilis / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D015151:Immunoblotting; D007527:Isoenzymes / Q000096:biosynthesis / Q000276:immunology; D010416:Pentachlorophenol / Q000494:pharmacology*; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000276:immunology; D051381:Rats
D000906:Antibodies; D004785:Environmental Pollutants; D007527:Isoenzymes; D015800:Protozoan Proteins; D003577:Cytochrome P-450 Enzyme System; D010416:Pentachlorophenol