title: Sorption of pyrene to dissolved humic substances and related model polymers. 2. Solid-phase microextraction (SPME) and fluorescence quenching technique (FHT) as analytical methods.
abstract: Sorption coefficients for pyrene on dissolved humic substances and on poly(acrylic acid) esters as well-defined model polymers were determined using solid-phase microextraction (SPME) and the fluorescence quenching technique (FQT). The results of both analytical methods were compared and theoretically evaluated, which led to the conclusion that the sorption coefficients measured by SPME and FQT are inevitably different: SPME measures activity-based and FQT concentration-based sorption coefficients. The environmental relevance of the two types of sorption coefficients is discussed. FQT is inappropriate to measure sorption coefficients for pyrene with the synthetic sorbents. Inspection of the vibrational structure of the fluorescence spectra of those solutions indicates a highly hydrophobic microenvironment of pyrene. This can be explained by an intra- or intermolecular agglomeration of hydrophobic moieties forming a favorable host for hydrophobic solutes.
authors.affiliation: Department of Remediation Research, UFZ-Center for Environmental Research Leipzig-Halle, Leipzig, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Mackenzie
authors.forename: Anett
authors.initials: A
authors.lastname: Georgi
authors.forename: Michael
authors.initials: M
authors.lastname: Kumke
authors.forename: Frank-Dieter
authors.initials: FD
authors.lastname: Kopinke
mesh_terms: D000327:Adsorption; D002623:Chemistry Techniques, Analytical / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D006812:Humic Substances / Q000737:chemistry*; D011108:Polymers; D011721:Pyrenes / Q000737:chemistry*; D012995:Solubility; D014873:Water Pollutants / Q000032:analysis
chemical_list: D005456:Fluorescent Dyes; D006812:Humic Substances; D011108:Polymers; D011721:Pyrenes; D014873:Water Pollutants; C030984:pyrene