A reference database for tumor-related genes co-expressed with interleukin-8 using genome-scale in silico analysis.
BACKGROUND
The EST database provides a rich resource for gene discovery and in silico expression analysis. We report a novel computational approach to identify co-expressed genes using EST database, and its application to IL-8.


RESULTS
IL-8 is represented in 53 dbEST cDNA libraries. We calculated the frequency of occurrence of all the genes represented in these cDNA libraries, and ranked the candidates based on a Z-score. Additional analysis suggests that most IL-8 related genes are differentially expressed between non-tumor and tumor tissues. To focus on IL-8's function in tumor tissues, we further analyzed and ranked the genes in 16 IL-8 related tumor libraries.


CONCLUSIONS
This method generated a reference database for genes co-expressed with IL-8 and could facilitate further characterization of functional association among genes.
Benbow
Lawrence
L
Discovery Technology Department, Schering-Plough Research Institute, 2015 Galloping Hill Rd, Kenilworth, NJ 07033, USA. lawrence.benbow@spcorp.com
Wang
Lynn
L
Laverty
Maureen
M
Liu
Suxing
S
Qiu
Ping
P
Bond
Richard W
RW
Gustafson
Eric
E
Hedrick
Joseph A
JA
Kostich
Mitchell
M
Greene
Jonathan R
JR
Wang
Luquan
L