title: Sex hormone binding globulin facilitates female sexual receptivity except when coupled to dihydrotestosterone.
abstract: Sex hormone binding globulin (SHBG) is produced in brain where it is often co-localized with oxytocin. Infusions of SHBG into the medial preoptic area-anterior hypothalamus facilitate female sexual receptivity. SHBG has receptors on plasma membranes of the prostate gland where binding of the 5alpha-reduced androgen dihydrotestosterone (DHT) by SHBG acts as an antagonist on SHBG receptors. This study attempted to determine whether pre-coupling DHT to SHBG would inhibit SHBG-induced facilitation of female sexual receptivity. Ovariectomized rats were injected daily with 0.75 microg estradiol benzoate for 3 days. On the fourth day after a pre-infusion baseline behavioral test animals were infused with 1 microl per side through bilateral cannulae with SHBG (1.77x10(-6) M), SHBG coupled to DHT (SHBG-DHT; 1.66x10(-6) M DHT), with DHT alone or with artificial cerebrospinal fluid vehicle. As before, SHBG significantly increased female sexual receptivity when infused into the medial preoptic area-anterior hypothalamus. Rats infused with SHBG-DHT had significantly lower sexual receptivity. Therefore, whereas SHBG in the medial preoptic area facilitated female sexual behavior, SHBG coupled to DHT did not. DHT itself did not significantly affect sexual receptivity. Pre-coupling DHT to SHBG eliminated the facilitative effect of SHBG on female sexual receptivity just as DHT inhibits SHBG activity at prostate SHBG receptors suggesting that central receptors for SHBG are similar to those demonstrated in the periphery.
authors.affiliation: Department of Biomedical Sciences, University of Illinois College of Medicine, 1601 Parkview Avenue, Rockford, IL 61107-1897, USA. jackc@uic.edu
authors.forename: Jack D
authors.initials: JD
authors.lastname: Caldwell
authors.forename: Simone
authors.initials: S
authors.lastname: Höfle
authors.forename: Ila
authors.initials: I
authors.lastname: Englöf
mesh_terms: D000818:Animals; D002404:Catheterization; D013196:Dihydrotestosterone / Q000008:administration & dosage / Q000378:metabolism*; D004333:Drug Administration Routes; D004334:Drug Administration Schedule; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology; D010052:Ovariectomy; D011187:Posture; D011301:Preoptic Area / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012738:Sex Hormone-Binding Globulin / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology*; D012726:Sexual Behavior, Animal / Q000187:drug effects* / Q000502:physiology
chemical_list: D012738:Sex Hormone-Binding Globulin; D013196:Dihydrotestosterone; C074283:estradiol 3-benzoate; D004958:Estradiol