title: In the immune synapse, ZAP-70 controls T cell polarization and recruitment of signaling proteins but not formation of the synaptic pattern.
abstract: Recognition by T cells of their ligands at the surface of antigen-presenting cells (APCs) leads to T cell activation, polarization of the T cell toward the APC, and formation of an immune synapse. Using ZAP-70-deficient T cells expressing zeta-GFP, we show that ZAP-70 signaling drives the TCR-dependent reorientation of the microtubule-organizing center thus leading to relocation of a zeta-GFP(+) intracellular compartment close to the APC. ZAP-70 is also necessary to supply the synapse with the signaling molecules PKC-theta and LAT. In contrast, ZAP-70 is not required for clustering of zeta-GFP and CD2 or exclusion of CD45 and CD43 from the synapse. These data show that ZAP-70-dependent signaling is required for formation of a functional immune synapse.
authors.affiliation: INSERM U520, Institut Curie, 12 Rue Lhomond, 75005 Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Blanchard
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Di Bartolo
authors.forename: Claire
authors.initials: C
authors.lastname: Hivroz
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000502:physiology; D015703:Antigens, CD; D018801:CD2 Antigens / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002450:Cell Communication; D002460:Cell Line; D016764:Cell Polarity; D049452:Green Fluorescent Proteins; D006801:Humans; D007365:Intercellular Junctions / Q000502:physiology; D007527:Isoenzymes / Q000378:metabolism; D019169:Jurkat Cells; D017493:Leukocyte Common Antigens / Q000378:metabolism; D051917:Leukosialin; D008164:Luminescent Proteins / Q000378:metabolism; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000378:metabolism; D022101:Microtubule-Organizing Center / Q000276:immunology / Q000502:physiology; D010750:Phosphoproteins / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D000074121:Protein Kinase C-theta; D011505:Protein-Tyrosine Kinases / Q000502:physiology*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology*; D051746:ZAP-70 Protein-Tyrosine Kinase
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015703:Antigens, CD; D018801:CD2 Antigens; D002352:Carrier Proteins; D007527:Isoenzymes; C110810:LAT protein, human; D051917:Leukosialin; D008164:Luminescent Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins; C465014:SPN protein, human; D012795:Sialoglycoproteins; C054979:antigen T cell receptor, zeta chain; D049452:Green Fluorescent Proteins; D011505:Protein-Tyrosine Kinases; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human; C076233:PRKCQ protein, human; D011493:Protein Kinase C; D000074121:Protein Kinase C-theta; D017493:Leukocyte Common Antigens