title: The correlation between CpG methylation and protein expression of P16 in oral squamous cell carcinomas.
abstract: We examined the P16 expression by immunohistochemical stain and detected the methylation by methylation specific polymerase chain reaction (MSP) in 48 primary oral squamous cell carcinoma (SCC) tissues. The results showed that 20/48 (41.7%) of cancerous tissues had CpG methylation around the promoter region, but 8/48 (17%) of the nearby non-cancerous tissues also had CpG methylation, around the promoter region. The results from immunohistochemical studies showed that reduced and heterogeneous expression of P16 were found in the tissues, which had CpG methylation around the promoter region. In conclusion, the methylation of P16 in oral SCC occurs in pre-cancerous and cancerous stage, which results in decreasing or abolishing the P16 expression, which is heterogeneous in the cancer cells.
authors.affiliation: Department of Molecular Medicine, China Medical College Hospital, Taichung, Taiwan, ROC.
authors.forename: Ming-Jer
authors.initials: MJ
authors.lastname: Huang
authors.forename: Kun-Tu
authors.initials: KT
authors.lastname: Yeh
authors.forename: Hung-Chang
authors.initials: HC
authors.lastname: Shih
authors.forename: Yu-Fen
authors.initials: YF
authors.lastname: Wang
authors.forename: Ting-Hsu
authors.initials: TH
authors.lastname: Lin
authors.forename: Jan-Yi
authors.initials: JY
authors.lastname: Chang
authors.forename: Mu-Chin
authors.initials: MC
authors.lastname: Shih
authors.forename: Jan-Gowth
authors.initials: JG
authors.lastname: Chang
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism*; D018899:CpG Islands; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D019942:Genes, p16; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism*; D016133:Polymerase Chain Reaction
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm