Global analysis of Brucella melitensis proteomes.
Brucella melitensis is a facultative, intracellular, gram-negative cocco-bacillus that causes Malta fever in humans and brucellosis in animals. There are at least six species in the genus, and the disease is classified as zoonotic because several species infect humans. Using 2-D gel electrophoresis and mass spectrometry, we have initiated (i) a comprehensive mapping and identification of all the expressed proteins of B. melitensis virulent strain 16M, and (ii) a comparative study of its proteome with the attentuated vaccinal strain Rev 1. Comprehensive proteome maps of all six Brucella species will be generated in order to obtain vital information for vaccine development, identification of pathogenicity islands, and establishment of host specificity and evolutionary relatedness.
Mujer
Cesar V
CV
Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510, USA.
Wagner
Mary Ann
MA
Eschenbrenner
Michel
M
Horn
Troy
T
Kraycer
Jo Ann
JA
Redkar
Rajendra
R
Hagius
Sue
S
Elzer
Philip
P
Delvecchio
Vito G
VG
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001428:Bacterial Vaccines; D002002:Brucella / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D002004:Brucella Vaccine; D017347:Brucella melitensis / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D002006:Brucellosis / Q000517:prevention & control / Q000662:veterinary; D002874:Chromosome Mapping / Q000662:veterinary; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D020543:Proteome / Q000032:analysis*; D013045:Species Specificity; D014774:Virulence; D015047:Zoonoses
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002004:Brucella Vaccine; D020543:Proteome