title: [Construction and expression of red fluorescent protein fusion vector incorporating p38 mitogen-activated protein kinase].
abstract: OBJECTIVE
  To construct the vector that expresses the fusion protein of p38 mitogen-activated protein kinase (MAPK) and red fluorescent protein (RFP) in mammalian cells.
  
  
  METHODS
  FLAG-tagged p38 MAPK in pcDNA3 vector was subcloned into RFP vector pDsRed1-N1, the construct of which was then transfected into HeLa cells and observed with fluorescence microscope.
  
  
  RESULTS
  The recombinant plasmid was verified by enzyme digestion, PCR and sequence analysis, and p38 MAPK-RFP fusion protein was highly expressed in HeLa cells. Fluorescence microscope found the red fluorescence distributed all over the cytoplasm and in the nuclei as well.
  
  
  CONCLUSIONS
  The expression vector for p38 MAPK-RFP fusion protein is successfully constructed and effective expression of this fusion protein is achieved, which might be instrumental in the study of intracellular localization of p38 MAPK.
authors.affiliation: Department of Pathophysiology and Key Laboratory of Shock and Microcirculation of PLA, First Military Medical University, Guangzhou 510515, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Gong
authors.forename: Qing-He
authors.initials: QH
authors.lastname: Qin
authors.forename: Jing-Zhen
authors.initials: JZ
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D015870:Gene Expression; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014162:Transfection; D048051:p38 Mitogen-Activated Protein Kinases; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D020928:Mitogen-Activated Protein Kinases; D011993:Recombinant Fusion Proteins; D048051:p38 Mitogen-Activated Protein Kinases; D000097573:Red Fluorescent Protein