title: Design and synthesis of activity probes for glycosidases.
abstract: [structure: see text] A new synthetic route was developed for the preparation of activity probe 1 for beta-glucosidase in this study. The key glycosidation step begins with benzyl p-hydroxyphenylacetate. Benzylic functionalization for the construction of the trapping device was achieved at later stages. Probe 1 was shown to be able to label the target enzyme. This cassette-like design offers great flexibility for future alterations. It would allow the synthetic scheme to expand to other glycosidase probes with different linker/reporter combinations.
authors.affiliation: Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Charng-Sheng
authors.initials: CS
authors.lastname: Tsai
authors.forename: Yaw-Kuen
authors.initials: YK
authors.lastname: Li
authors.forename: Lee-Chiang
authors.initials: LC
authors.lastname: Lo
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D015335:Molecular Probes; D001617:beta-Glucosidase