title: Production of ligninolytic enzymes for dye decolorization by cocultivation of white-rot fungi Pleurotus ostreatus and phanerochaete chrysosporium under solid-state fermentation.
abstract: Lignocellulosic wastes such as neem hull, wheat bran, and sugarcane bagasse, available in abundance, are excellent substrates for the production of ligninolytic enzymes under solid-state fermentation by white-rot fungi. A ligninolytic enzyme system with high activity showing enhanced decomposition was obtained by cocultivation of Pleurotus ostreatus and Phanerochaete chrysosporium on combinations of lignocellulosic waste. Among the various substrate combinations examined, neem hull and wheat bran wastes gave the highest ligninolytic activity. A maximum production of laccase of 772 U/g and manganese peroxidase of 982 U/g was obtained on d 20 and lignin peroxidase of 656 U/g on d 25 at 28 +/- 1 degrees C under solid-state fermentation. All three enzymes thus obtained were partially purified by acetone fractionation and were exploited for decolorizing different types of acid and reactive dyes.
authors.affiliation: Post-Graduate Department of Biosciences, Sardar Patel University, Gujarat, India.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Verma
authors.forename: Datta
authors.initials: D
authors.lastname: Madamwar
mesh_terms: D001673:Biodegradation, Environmental; D005591:Chemical Fractionation / Q000379:methods; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D005285:Fermentation; D007220:Industrial Waste; D042845:Laccase; D008031:Lignin / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010088:Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D010544:Peroxidases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology*; D020076:Pleurotus / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004396:Coloring Agents; D007220:Industrial Waste; D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase