Use of an adenosine triphosphate-based 'cocktail' early in reperfusion substantially improves brain protein synthesis after global ischemia in rats.
Neurological damage is a serious problem after cardiac arrest and resuscitation. We used a rat cardiac arrest model to test the ability of a post-ischemic infusion of adenosine triphosphate-magnesium chloride (ATP-MgCl(2)) to sustain cortical protein synthesis after 7 min global ischemia. We used norepinephrine (NE) to block the vasodilatory action of ATP, and a trace of vanadate to simulate the equine-derived ATP Chaudry used to protect against ischemia or hemorrhage in other organs. Our ATP 'cocktail' (3 ml of 4 mg/ml ATP-MgCl(2), 18 microg/ml NE, 2.4 microg/ml vanadate, infused intravenously over 18 min) tripled post-ischemic protein synthesis. ATP without vanadate, and vanadate without ATP, both had lesser but still significant effects. This ATP 'cocktail' may be useful as a neuroprotectant after cardiac arrest and resuscitation.
Paskitti
Megan
M
Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY 40292, USA.
Reid
Kenneth H
KH
D000255:Adenosine Triphosphate / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D006323:Heart Arrest / Q000503:physiopathology; D007262:Infusions, Intravenous; D007511:Ischemia / Q000188:drug therapy*; D008297:Male; D009638:Norepinephrine / Q000008:administration & dosage / Q000627:therapeutic use; D014176:Protein Biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D013997:Time Factors; D014638:Vanadates / Q000008:administration & dosage / Q000627:therapeutic use; D014662:Vasoconstrictor Agents / Q000008:administration & dosage / Q000627:therapeutic use
D014662:Vasoconstrictor Agents; D014638:Vanadates; D000255:Adenosine Triphosphate; D009638:Norepinephrine