title: AFM structural study of the molecular chaperone GroEL and its two-dimensional crystals: an ideal "living" calibration sample.
abstract: Supramolecular complexes, such as chaperonins, are suitable samples for atomic force microscope structural studies because they have a very well defined shape. High-resolution images can be made using tapping mode in liquid under native conditions. Details about the two-dimensional structures formed onto the surface upon adsorption and of the single protein can be observed. Dissection of the upper ring of the supramolecular complex as a result of the applied lateral force through scanning tip is observed. Finally, the combination of lateral convolution and tip penetration into the cavity of chaperonins offers a direct evaluation of the tip convolution effect on images of macromolecular samples.
authors.affiliation: Institut de Physique de la Matière Condensèe, BSP, Université de Lausanne, Dorigny, Switzerland. francesco.valle@ipmc.unil.ch
authors.forename: F
authors.initials: F
authors.lastname: Valle
authors.forename: J A
authors.initials: JA
authors.lastname: DeRose
authors.forename: G
authors.initials: G
authors.lastname: Dietler
authors.forename: M
authors.initials: M
authors.lastname: Kawe
authors.forename: A
authors.initials: A
authors.lastname: Plückthun
authors.forename: G
authors.initials: G
authors.lastname: Semenza
mesh_terms: D002138:Calibration; D018834:Chaperonin 60 / Q000737:chemistry*; D003460:Crystallization; D007091:Image Processing, Computer-Assisted; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D018832:Molecular Chaperones / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D018834:Chaperonin 60; D018832:Molecular Chaperones