title: Immunologically driven chemical engineering of antibodies for catalytic activity.
abstract: We describe a new strategy for the preparation of catalytic antibodies based on a two-step procedure. Firstly, monoclonal antibodies are selected only if displaying the following binding features: binding both the substrate and a reactive group in such a way that the two groups are in a reactive position towards each other. Secondly, the selected monoclonal antibodies (mAbs) are chemically engineered by covalently binding the reactive group into the binding pocket of the antibody. Using previously isolated monoclonal antibodies, we have focused our studies on the control of this second step.
authors.affiliation: Service de Pharmacologie et d'Immunologie, DRM/DSV CEA Saclay, 91191 Gif sur Yvette cedex, France.
authors.forename: Sonia
authors.initials: S
authors.lastname: Dias
authors.forename: Florence
authors.initials: F
authors.lastname: Jovic
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Renard
authors.forename: Fréderic
authors.initials: F
authors.lastname: Taran
authors.forename: Christophe
authors.initials: C
authors.lastname: Créminon
authors.forename: Charles
authors.initials: C
authors.lastname: Mioskowski
authors.forename: Jacques
authors.initials: J
authors.lastname: Grassi
mesh_terms: D000110:Acetylcholinesterase / Q000276:immunology*; D000818:Animals; D017151:Antibodies, Catalytic / Q000096:biosynthesis* / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D017151:Antibodies, Catalytic; D000911:Antibodies, Monoclonal; D000110:Acetylcholinesterase