title: Failure to demonstrate involvement of antibodies to Acinetobacter calcoaceticus in transmissible spongiform encephalopathies of animals.
abstract: Acinetobacter calcoaceticus, a soil microbe, contains molecular sequences which resemble those found in neurofilaments of the brain tissue. It was hypothesized that if cattle ingest large amounts of feedstuff containing A. calcoaceticus, they may develop an autoimmune reaction, with consequences of pathological changes associated with transmissible spongiform encephalopathies (TSEs). The hypothesis was tested using a small number of serum samples collected from cattle and it was found that affected individuals had elevated serum antibody levels to this organism. If this finding was substantiated, it would provide a possible means of diagnosing TSEs in vivo. In the present communication, a larger number of cattle, elk and sheep with or without TSEs were tested using A. calcoaceticus whole cell and lipopolysaccharide antigens as well as myelin basic protein (MBP). It was found that antibody levels in normal and affected animals overlapped considerably, thus casting doubt on the usefulness of these antigens as diagnostic tools for TSEs and on the hypothesis of A. calcoaceticus being a cause of TSEs.
authors.affiliation: Animal Diseases Research Institute, Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, Ont, Canada K2H 8P9. nielsenk@inspection.gc.ca
authors.forename: K
authors.initials: K
authors.lastname: Nielsen
authors.forename: J
authors.initials: J
authors.lastname: Widdison
authors.forename: A
authors.initials: A
authors.lastname: Balachandran
authors.forename: D
authors.initials: D
authors.lastname: Stevenson
authors.forename: J
authors.initials: J
authors.lastname: Algire
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood / Q000276:immunology; D002417:Cattle / Q000276:immunology / Q000382:microbiology; D003670:Deer / Q000276:immunology / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D017096:Prion Diseases / Q000276:immunology* / Q000382:microbiology*; D012756:Sheep / Q000276:immunology / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial