title: Molecular characterization of bla(IMP-5), a new integron-borne metallo-beta-lactamase gene from an Acinetobacter baumannii nosocomial isolate in Portugal.
abstract: Acinetobacter baumannii 65FFC, an imipenem-resistant clinical strain, isolated from the urine of a patient at the Coimbra University Hospital, Portugal, in 1998, produced a metallo-beta-lactamase with a calculated pI 9.3. The isolate was highly resistant to penicillins, broad-spectrum cephalosporins, including ceftazidime, ceftriaxone, cefepime, cefpirome, and to aztreonam, but it remained susceptible to ampicillin/sulbactam, aminoglycosides and quinolones. Nucleotide sequence revealed a new allelic variant of other bla(IMP) genes, named bla(IMP-5). IMP-5 beta-lactamase showed a greater homology with IMP-1, IMP-3 and IMP-4 (identified in Southeast Asia), than with IMP-2, found in Italy (93%, 92%, 91% and 87% of amino acid identity, respectively). bla(IMP-5) was the only gene cassette inserted into a class 1 integron, named In76. This is the first IMP-enzyme reported in Portugal and the second in Europe, indicating a wider dissemination in the environment of bla(IMP) alleles.
authors.affiliation: Laboratory of Microbiology, Faculty of Pharmacy and Center of Pharmaceutical Studies, University of Coimbra, Couraça dos Apóstolos, 51, r/c E, 3030, Coimbra, Portugal. gjsilva@ci.uc.pt
authors.forename: Gabriela J
authors.initials: GJ
authors.lastname: Da Silva
authors.forename: Mário
authors.initials: M
authors.lastname: Correia
authors.forename: Clementina
authors.initials: C
authors.lastname: Vital
authors.forename: Graça
authors.initials: G
authors.lastname: Ribeiro
authors.forename: João C
authors.initials: JC
authors.lastname: Sousa
authors.forename: Rui
authors.initials: R
authors.lastname: Leitão
authors.forename: Luísa
authors.initials: L
authors.lastname: Peixe
authors.forename: Aida
authors.initials: A
authors.lastname: Duarte
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology* / Q000652:urine; D040981:Acinetobacter baumannii / Q000235:genetics*; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents; D001483:Base Sequence; D003001:Cloning, Molecular; D003428:Cross Infection / Q000382:microbiology* / Q000652:urine; D006801:Humans; D015378:Imipenem; D032023:Integrons / Q000235:genetics; D008969:Molecular Sequence Data; D011174:Portugal; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; D001618:beta-Lactamases