title: Ammonium detoxifying activity is maintained after 72 hours of cold preservation of rat hepatocytes in University of Wisconsin (UW) solution.
abstract: The ammonium removal efficiency (ARE) and cell viability was investigated in freshly isolated rat hepatocytes exposed to increasing ammonium loads (0.1-2.0 mM). No difference was observed in both ARE and cell viability at the different ammonium concentrations tested. Storage of hepatocytes at 0 degrees C for 72 hours inhibited ammonium removal and urea synthesis. Rewarming of cells at 37 degrees C for 120 min was followed by an ARE fully comparable to freshly isolated hepatocytes. These data indicated that cold preservation of rat hepatocytes for 3 days in UW followed by a rewarming is associated with normal ammonium detoxification efficiency.
authors.affiliation: Departamento de Ciencias Fisiologicas, Facultad de Ciencias, Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Suipacha 531-2000 Rosario (S2002LRK), Argentina.
authors.forename: Sebastián D
authors.initials: SD
authors.lastname: Calligaris
authors.forename: Luciana L
authors.initials: LL
authors.lastname: Almada
authors.forename: Edgardo E
authors.initials: EE
authors.lastname: Guibert
authors.forename: Claudio
authors.initials: C
authors.lastname: Tiribelli
authors.forename: Joaquin V
authors.initials: JV
authors.lastname: Rodriguez
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D015925:Cryopreservation; D005978:Glutathione; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D007328:Insulin; D008297:Male; D019188:Organ Preservation Solutions; D000644:Quaternary Ammonium Compounds / Q000378:metabolism / Q000494:pharmacology*; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; D000644:Quaternary Ammonium Compounds; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose