title: The origin of a developmentally regulated Igh replicon is located near the border of regulatory domains for Igh replication and expression.
abstract: The 3' Ig heavy chain locus (Igh) regulatory region is the most downstream known element of the murine Igh gene cluster. We report here that the nearest non-Igh genes-Crip, Crp2, and Mta1-are located approximately 70 kb further downstream and are beyond the end of the domain of Igh transcriptional regulation. We have localized an origin of replication in MEL cells to a 3-kb segment located between the 3' Igh regulatory region and Crip. Sequences downstream of this origin are replicated by forks that move in both directions. Sequences upstream of this origin (Igh-C, -D, and -J) are replicated in a single direction through a 500-kb segment in which no active bidirectional origins can be detected. We propose that this origin may lie at or near the end of the Igh regulation domain.
authors.affiliation: Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Nasrin
authors.initials: N
authors.lastname: Ashouian
authors.forename: Marc
authors.initials: M
authors.lastname: Delepine
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Matsuda
authors.forename: Christophe
authors.initials: C
authors.lastname: Chevillard
authors.forename: Roy
authors.initials: R
authors.lastname: Riblet
authors.forename: Carl L
authors.initials: CL
authors.lastname: Schildkraut
authors.forename: Barbara K
authors.initials: BK
authors.lastname: Birshtein
mesh_terms: D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D002460:Cell Line; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation; D006655:Histone Deacetylases; D007070:Immunoglobulin A / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D060588:LIM Domain Proteins; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011506:Proteins / Q000235:genetics; D018741:Replication Origin; D012093:Replicon / Q000235:genetics*; D012097:Repressor Proteins; D015534:Trans-Activators
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; C486181:CRIP1 protein, human; D002352:Carrier Proteins; C489507:Crip1 protein, mouse; D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains; D060588:LIM Domain Proteins; C089320:MTA1 protein, human; D011506:Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; D006655:Histone Deacetylases