title: A model for interphase chromosomes and evaluation of radiation-induced aberrations.
abstract: We have developed a theoretical model for evaluating radiation-induced chromosomal exchanges by explicitly taking into account interphase (G(0)/G(1)) chromosome structure, nuclear organization of chromosomes, the production of double-strand breaks (DSBs), and the subsequent rejoinings in a faithful or unfaithful manner. Each of the 46 chromosomes for human lymphocytes (40 chromosomes for mouse lymphocytes) is modeled as a random polymer inside a spherical volume. The chromosome spheres are packed randomly inside a spherical nucleus with an allowed overlap controlled by a parameter Omega. The rejoining of DSBs is determined by a Monte Carlo procedure using a Gaussian proximity function with an interaction range parameter sigma. Values of Omega and sigma have been found which yield calculated results of interchromosomal aberration frequencies that agree with a wide range of experimental data. Our preferred solution is one with an interaction range of 0.5 microm coupled with a relatively small overlap parameter of 0.675 microm, which more or less confirms previous estimates. We have used our model with these parameter values and with resolution or detectability limits to calculate yields of translocations and dicentrics for human lymphocytes exposed to low-LET radiation that agree with experiments in the dose range 0.09 to 4 Gy. Five different experimental data sets have been compared with the theoretical results. Essentially all of the experimental data fall between theoretical curves corresponding to resolution limits of 1 Mbp and 20 Mbp, which may reflect the fact that different investigators use different limits for sensitivity or detectability. Translocation yields for mouse lymphocytes have also been calculated and are in good agreement with experimental data from 1 cGy to 10 cGy. There is also good agreement with recent data on complex aberrations. Our model is expected to be applicable to both low- and high-LET radiation, and we include a sample prediction of the yield of interchromosomal rejoining in the dose range 0.22 Gy to 2 Gy of 1000 MeV/nucleon iron particles. This dose range corresponds to average particle traversals per nucleus ranging from 1.0 to 9.12.
authors.affiliation: Lawrence Berkeley National Laboratory, Life Sciences Division, University of California, Berkeley, California 94720, USA.
authors.forename: W R
authors.initials: WR
authors.lastname: Holley
authors.forename: I S
authors.initials: IS
authors.lastname: Mian
authors.forename: S J
authors.initials: SJ
authors.lastname: Park
authors.forename: B
authors.initials: B
authors.lastname: Rydberg
authors.forename: A
authors.initials: A
authors.lastname: Chatterjee
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics / Q000528:radiation effects; D002869:Chromosome Aberrations / Q000528:radiation effects*; D019457:Chromosome Breakage; D033481:Chromosomes, Mammalian / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects; D006801:Humans; D007399:Interphase / Q000528:radiation effects*; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects; D008433:Mathematics; D051379:Mice; D008962:Models, Theoretical; D011829:Radiation Dosage; D011995:Recombination, Genetic / Q000528:radiation effects