title: [Analysis of CD8(+) and CD8(+)CD28(-) cell subsets in patients with hepatocellular carcinoma].
abstract: OBJECTIVE
  To study the expression of the costimulatory molecule CD28 on peripheral blood CD8(+)T cells from patients with hepatocellular carcinoma (HCC).
  
  
  METHODS
  The expression of CD28 on CD8(+), CD8(+)CD28(-)- and CD8(+)CD28(+) cell subsets in the peripheral blood obtained from 20 HCC patients and 15 healthy controls were assayed by flow cytometry.
  
  
  RESULTS
  The elevated percentages of CD8(+) cells, decreased percentages of CD8(+)CD28(+) cells and increased percentages of CD8(+)CD28(-)- cells were observed in the peripheral blood of HCC patients as compared with those of the control group (P<0.01). Positive correlation between the percentage of CD8(+)CD28(-)- cells and that of CD8(+) cells were found.
  
  
  CONCLUSIONS
  CD28 expression on CD8(+) T cells was aberrant in the peripheral blood of HCC patients, and the elevation of CD8(+)CD28(-)- cell number results in the increase of CD8(+) cell number.
authors.affiliation: Clinical Laboratory, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Yu-Rong
authors.initials: YR
authors.lastname: Qiu
authors.forename: Chun-Li
authors.initials: CL
authors.lastname: Yang
authors.forename: Lian-Bo
authors.initials: LB
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000328:Adult; D018106:CD28 Antigens / Q000032:analysis* / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology*; D008297:Male; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D018106:CD28 Antigens