Stability of total selenium and selenium species in lyophilised oysters and in their enzymatic extracts.
To obtain reliable information on speciation analysis it is necessary to previously evaluate the stability of the species in the sample of interest. Furthermore, in those cases in which sample treatment to extract the species is time-consuming, an evaluation of how to maintain species integrity in the extracts is paramount. Thus, the present paper reports the stability of total Se, SeMet and TMSe+ in freeze-dried oyster and in the enzymatic extracts stored in Pyrex and polyethylene containers at different temperatures (-18, 4 and 20 degrees C). Total selenium determinations and Se speciation were carried out by HG-AAS after acid digestion in a microwave oven and by on-line coupling of cation exchange HPLC-ICP-MS after enzymatic hydrolysis, respectively. The results obtained for the freeze-dried sample showed that total Se and the selenium species evaluated are stable for at least 12 months, under all the conditions tested. However, Se species in the enzymatic extracts are only stable for 10 days if stored at 4 degrees C in Pyrex containers. These results show that the extracts do not necessarily have to be analysed just after sample treatment.
Moreno
P
P
Departamento de Química Analítica, Facultad de C.C. Químicas, Universidad Complutense, Ciudad Universitaria s/n, 28040 Madrid, Spain.
Quijano
M A
MA
Gutiérrez
A M
AM
Pérez-Conde
M C
MC
Cámara
C
C
D000818:Animals; D005612:Freeze Drying / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010125:Ostreidae / Q000737:chemistry* / Q000201:enzymology*; D012643:Selenium / Q000032:analysis*; D018036:Selenium Compounds / Q000032:analysis*; D012645:Selenomethionine / Q000032:analysis*; D013054:Spectrophotometry, Atomic / Q000379:methods*; D020860:Subtilisin / Q000378:metabolism
D018036:Selenium Compounds; D012645:Selenomethionine; D020860:Subtilisin; D012643:Selenium; C003717:trimethylselenonium