Comparison of the activities of wild type and mutant enhancing factor/mouse secretory phospholipase A2 proteins.
Enhancing factor (EF) protein, an isoform of secretory phospholipase A2 (PLA2), was purified as a modulator of epidermal growth factor from the small intestine of the Balb/c mouse. It was for the first time that a growth modulatory property of sPLA2 was demonstrated. Deletion mutation analysis of EF cDNA carried out in our laboratory showed that enhancing activity and phospholipase activity are two separate activities that reside in the same molecule. In order to study the specific amino acids involved in each of these activities, two site-directed mutants of EF were made and expressed in vitro. Comparison of enhancing activity as well as phospholipase A2 activity of these mutant proteins with that of wild type protein helped in identification of some of the residues important for both the activities
Kirtane
Bhakti M
BM
Laboratory of Genetic Engineering, Cancer Research Institute, Tata Memorial Centre, Parel, Mumbai 400 012, India.
Mulherkar
Rita
R
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D017931:DNA Primers; D054501:Group II Phospholipases A2; D006801:Humans; D051379:Mice; D016297:Mutagenesis, Site-Directed; D010741:Phospholipases A / Q000235:genetics / Q000378:metabolism*; D054467:Phospholipases A2
D017931:DNA Primers; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2; C516288:Pla2g2a protein, mouse