title: Antitumor effect of radioimmunotherapy in a mouse model of testicular tumor with micrometastases defined by polymerase chain reaction.
abstract: The efficacy of radioimmunotherapy (RIT) was evaluated by using a mouse model of testicular tumor with macro- and micrometastases to various organs. RIT consisting of a single intravenous injection of 5.8 MBq of I-131-labeled anti-placental alkaline phosphatase (PLAP) MAb was conducted one day or 7 days after testicular implantation in SCID mice of HeLa Hep2 cells expressing PLAP. RIT antitumor effect was significant for primary tumors as well as micrometastases defined by polymerase chain reaction (PCR) assay. However, ablation of tumor cells could be achieved with this treatment only at the initial stage of tumor growth in the testis, when metastasis could also be prevented. Once micrometastasis had occurred, however, complete elimination of tumor cell(s) was difficult. These findings appear to have implications for the use of RIT in treatment for micrometastasis, especially when detectable only by PCR assay.
authors.affiliation: Department of Urology, School of Medicine, Kanazawa University, Kanazawa 920-8640, Japan. tak1019@kenroku.kanazawa-u.ac.jp
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Koshida
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Endo
authors.forename: Yoshihumi
authors.initials: Y
authors.lastname: Kadono
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hirano
authors.forename: Takuma
authors.initials: T
authors.lastname: Sasaki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mizokami
authors.forename: Mikio
authors.initials: M
authors.lastname: Namiki
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000556:secondary / Q000628:therapy*; D000469:Alkaline Phosphatase / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis; D005914:Globins / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D007457:Iodine Radioisotopes / Q000627:therapeutic use*; D008113:Liver Neoplasms / Q000235:genetics / Q000556:secondary / Q000628:therapy*; D008175:Lung Neoplasms / Q000235:genetics / Q000556:secondary / Q000628:therapy*; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D018365:Neoplasm, Residual / Q000276:immunology / Q000628:therapy; D010920:Placenta / Q000201:enzymology; D016133:Polymerase Chain Reaction; D016499:Radioimmunotherapy; D013736:Testicular Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D014183:Transplantation, Heterologous
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D004273:DNA, Neoplasm; D007457:Iodine Radioisotopes; D005914:Globins; D000469:Alkaline Phosphatase