Engineering the refolding pathway and the quaternary structure of seminal ribonuclease by newly introduced disulfide bridges.
Seminal RNase (BS-RNase), a ribonuclease from bovine seminal vesicles, is a homodimeric enzyme with a strong cytotoxic activity selective for tumor cells. It displays the unusual structural feature of existing in solution as an equilibrium mixture of two quaternary isoforms. The major one is characterized by the swap between subunits of their N-terminal ends, whereas the minor isoform shows no swap. The tendency of the two isolated isoforms to interconvert into each other has so far made it difficult to attribute the functional properties of BS-RNase to either isoform. Herein, molecular modeling and site-directed mutagenesis were used to engineer the refolding pathway of BS-RNase and obtain a stable variant of its non-swapping isoform. The protein was engineered with two extra disulfide bridges linking the N-terminal helix of each subunit to the main body of the same subunit. Purified as an active enzyme, the BS-RNase variant was found to be very resistant to thermal denaturation. Its functional characterization revealed that the lack of swapping has a negative effect on the cytotoxic activity of BS-RNase.
Russo
Aniello
A
Department of Life Sciences, Second University of Naples, Via Vivaldi 43, 81100 Caserta, Italy.
Antignani
Antonella
A
Giancola
Concetta
C
D'Alessio
Giuseppe
G
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002942:Circular Dichroism; D017931:DNA Primers; D004220:Disulfides / Q000737:chemistry*; D004795:Enzyme Stability; D008297:Male; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D012260:Ribonucleases / Q000737:chemistry* / Q000235:genetics; D012669:Seminal Vesicles / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet
D017931:DNA Primers; D004220:Disulfides; D012260:Ribonucleases