[Preliminary study of development of gene chips for HIV diagnosis].
OBJECTIVE
To study the technology for establishing DNA chips for the diagnosis of HIV.


METHODS
HIV 1U26942 DNA fragments were isolated by restriction display-PCR (RD-PCR) and printed onto aminosilane-coated glass slides by Pixsys 5500 arrayer as probes to prepare the gene chips. HIV samples, after labeled with Cy3, were hybridized with the microarray followed by scanning for analysis of hybridization kinetics of the RD fragments.


RESULTS
The experimental condition for preparing the gene chips was investigated and 12 RD fragments were screened as probes for further study.


CONCLUSIONS
The technique established in this study for preparing DNA chips is specific and applicable.
Li
Ling
L
Institute of Molecular Biology and Key Laboratory of Biochip of PLA, First Military Medical University, Guangzhou 510515, China. liling@fimmu.edu.cn
Ma
Wen-Li
WL
Mao
Xiang-Ming
XM
Zhu
Ji
J
Zheng
Wen-Ling
WL
D019937:Diagnostic Techniques and Procedures; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012680:Sensitivity and Specificity