[Detection of apoptosis with gel eletrophoresis combined with computerized gel analysis system].
OBJECTIVE
To explore a new method for detecting cellular apoptosis.


METHODS
Using routine electrophoresis assisted by computerized gel documentation analysis system(GDAS), apoptosis of the macrophages treated with dexamethasone was qualitatively and then quantitatively detected.


RESULTS
Apoptosis occurred in the macrophages treated with dexamethasone, and DNA fragmentation of the apoptotic macrophages was visualized on 1% agarose gel electrophoresis. The apoptosis rates as determined by computerized GDAS were consistent with the results obtained from flow cytometry that showed typical apoptosis peak.


CONCLUSIONS
Agarose gel electrophoresis assisted by computerized GDAS was relatively simple and less costly in qualitative and quantitative detection of apoptosis with precision.
Zhao
Qing
Q
Central Laboratory, First Military Medical University, Guangzhou 510515, China.
Wu
Jin-Ya
JY
Huang
Xing-Xu
XX
Ma
Xiao-Dong
XD
Bao
Yong-Yao
YY
Zhou
Jun-Ling
JL
D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D051379:Mice
D003907:Dexamethasone