title: Ile-Lys-Val-Ala-Val (IKVAV)-containing laminin alpha1 chain peptides form amyloid-like fibrils.
abstract: The Ile-Lys-Val-Ala-Val (IKVAV) sequence derived from laminin-1 promotes cell adhesion, neurite outgrowth, and tumor growth and metastasis. Here, we examined amyloid formation of an IKVAV-containing peptide (LAM-L: AASIKVAVSADR, mouse laminin alpha1 chain 2097-2108). The LAM-L peptide was stained with Congo red and exhibited fibrils in electron microscopy with a characteristic cross-beta X-ray diffraction pattern. Further, infrared spectra of LAM-L suggested a beta-sheet structure. These results indicate that LAM-L forms amyloid-like fibrils. We also examined amyloid-like fibril formation of LAM-L analogs. The neurite outgrowth activity of the LAM-L analogs was closely related to their amyloid-like fibril formation.
authors.affiliation: Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Kita 10 Nishi 5, Kita-ku, Sapporo 060-0810, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Yamada
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kadoya
authors.forename: Shingo
authors.initials: S
authors.lastname: Kasai
authors.forename: Kozue
authors.initials: K
authors.lastname: Kato
authors.forename: Mayumi
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Norio
authors.initials: N
authors.lastname: Nishi
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Watanabe
authors.forename: Hynda K
authors.initials: HK
authors.lastname: Kleinman
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Yamada
authors.forename: Motoyoshi
authors.initials: M
authors.lastname: Nomizu
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D003224:Congo Red; D007797:Laminin / Q000737:chemistry*; D008854:Microscopy, Electron; D010446:Peptide Fragments / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D000682:Amyloid; D007797:Laminin; D010446:Peptide Fragments; C083321:isoleucyl-lysyl-valyl-alanyl-valine; C084098:laminin A; D003224:Congo Red