title: Use of a resorbable sheet in iliac crest reconstruction in a sheep model.
abstract: Iliac crest bone graft harvesting can result in major complications that occur at rates of approximately 6%-8%. The objective of this study was to evaluate the postoperative regeneration of iliac crest donor defects in an animal model after harvesting a full-thickness tricortical graft. In skeletally mature sheep, a tricortical iliac crest graft was harvested. The graft sites were allowed to heal nonprotected or protected with the resorbable polylactic acid sheet material, MacroPore OS Protective Sheeting, (MacroPore Biosurgery, San Diego, Calif). After 6 months of healing, the sites were assessed by examination of undecalcified histologic sections. Histomorphometric measurements of the original defect area, the area of new bone within the defect site, and the percentage of defect filled with new bone were quantified for both control and protected groups. In all histologic sections, new bone growth within the defect sites appeared normal, with no excessive inflammatory cells observed. The developing bone tissue appeared to be remodeling normally. For the nonprotected sites, the area of new bone averaged 16.3 mm2 (+/-7.2 mm2), and the percentage of the defect area filled with bone averaged 10.7% (+/-6.5%). In the protected sites, the area of new bone averaged 64.8 mm2 (+/-11.6 mm2) and the percentage of the defect area filled with bone averaged 25.9% (+/-1.6%). Both differences in area of new bone growth and percentage of defect area filled were statistically significant. Literature review has indicated that regeneration of donor site defects is desirable. Based upon the results of the present study, MacroPore resorbable protective sheeting can improve bone regeneration significantly within the donor site following tricortical iliac crest graft harvesting.
authors.affiliation: MacroPore Biosurgery, Inc, San Diego, Calif, USA.
authors.forename: G Bryan
authors.initials: GB
authors.lastname: Cornwall
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Thomas
authors.forename: A Simon
authors.initials: AS
authors.lastname: Turner
authors.forename: Donna L
authors.initials: DL
authors.lastname: Wheeler
authors.forename: William R
authors.initials: WR
authors.lastname: Taylor
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001707:Biopsy, Needle; D016025:Bone Transplantation / Q000379:methods*; D005260:Female; D006084:Graft Rejection; D006085:Graft Survival; D007085:Ilium / Q000473:pathology* / Q000637:transplantation*; D007150:Immunohistochemistry; D008297:Male; D023421:Models, Animal; D011336:Probability; D019651:Plastic Surgery Procedures / Q000295:instrumentation* / Q000379:methods; D012016:Reference Values; D012680:Sensitivity and Specificity; D012756:Sheep; D020858:Tissue and Organ Harvesting; D014182:Transplantation, Autologous