Pan-Mediterranean comparison for the molecular detection of Theileria annulata.
Seven laboratories decided to compare their molecular diagnostic techniques to identify Mediterranean theileriosis caused by Theileria annulata. Each laboratory used either PCR or PCR and reverse line blot hybridization (RLB) to identify T. annulata. Five laboratories sent their own samples to laboratory 4 to be recoded and passed on to at least two other laboratories. A total of 120 blood samples were analyzed during this study, generating 540 results. Laboratory 1 sent only T. annulata-infected samples (positive control batch), and all the laboratories testing this batch found 100% infection. Laboratory 2 sent only negative samples from a Mediterranean area where T. annulata was unknown, and two laboratories out of three found a few positive samples in these negative samples. For the remaining samples, detection performance was variable. Agreement between laboratories ranged from 21.4 to 91.3%. The overall mismatch between laboratories was around 30% by whatever technique used. This paper describes the methodological parameters that could explain the variation of results.
Sparagano
Olivier A E
OA
School of Agriculture, Food and Rural Development, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK. olivier.sparagano@ncl.ac.uk
Carelli
Grazia
G
Ceci
Luigi
L
Shkap
Varda
V
Molad
Thea
T
Vitale
Fabrizio
F
Loria
Guido R
GR
Reale
Stefano
S
Caracappa
Santo
S
Bouattour
Ali
A
Almeria
Sonia
S
Castella
Joaquim
J
Corchero
Eduardo
E
Habela
Miguel
M
D000818:Animals; D002417:Cattle; D015342:DNA Probes; D005189:False Positive Reactions; D005784:Gene Amplification; D019083:Mediterranean Region / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D016796:Theileria annulata / Q000235:genetics / Q000302:isolation & purification*; D013801:Theileriasis / Q000175:diagnosis* / Q000453:epidemiology
D015342:DNA Probes