[Experimental studies on cranial defect repair with marrow stromal osteoblast-cancellous bone matrix compound artificial bone].
OBJECTIVE
To investigate the feasibility of using marrow stromal osteoblast-cancellous bone matrix compound artificial bone (MCCAB) as tissue-engineered bone, the osteogenesis of MCCAB in the cranial defect was observed in the experiment.


METHODS
The in vitro cultivated and induced marrow stromal cells of adult New Zealand rabbits were seeded into the alginate-cancellous bone matrix to form MCCAB. The MCCAB was then implanted into the cranial defect for 4 to 8 weeks. The cancellous bone matrix (CBM) alone or the marrow stromal osteoblasts (MSOs) alone was implanted as the control. The effectiveness of bone formation was assessed by histological and roentgenographic analysis.


RESULTS
The osteogenesis of MCCAB was better than CBM or MSOs and superior to the blank group.


CONCLUSIONS
MCCAB can effectively repair cranial defect. It could be used clinically to restore large bone defects.
Tao
Kai
K
Laboratory of Tissue Engineering, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
Mao
Tianqiu
T
Yang
Weidong
W
Chen
Fulin
F
Gu
Xiaoming
X
Chen
Shujun
S
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D001857:Bone Matrix / Q000166:cytology; D002478:Cells, Cultured; D005240:Feasibility Studies; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D011817:Rabbits; D012886:Skull / Q000002:abnormalities*; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology*