The ratio of plasminogen activator inhibitor type-1 activity to the concentration of plasminogen activator inhibitor type-1 protein in diabetes: adding insult to injury.
BACKGROUND
This study was performed to delineate the relationship between plasminogen activator inhibitor type-1 (PAI-1) activity and PAI-1 protein to characterize the functional importance of elevated PAI-1 in diabetes.


METHODS
PAI-1 activity (chromogenic substrate kinetic assay) and protein (enzyme-linked immunosorbent assay) were assayed in baseline central venous catheter-acquired samples from 170 patients with type 2 diabetes studied in the Troglitazone Atherosclerosis Regression Trial (TART).


RESULTS
The data demonstrated that the ratio of PAI-1 activity to the concentration of PAI-1 protein is increased as a function of increasing concentrations of PAI-1 protein in blood.


CONCLUSIONS
The results demonstrate that the ratio of PAI-1 activity to the concentration of PAI-1 protein is higher when the concentration of PAI-1 is elevated. The nonlinearity appears to depend on the fact that the rate of disappearance of PAI-1 activity in blood is a function of the concentration of PAI-1 protein.
Sobel
Burton E
BE
Department of Medicine, University of Vermont College of Medicine, Burlington, Vermont, USA. burt.sobel@vtnednet.org
Neimane
Dagnija
D
Mack
Wendy J
WJ
Hodis
Howard N
HN
Buchanan
Thomas A
TA
D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood*; D010960:Plasminogen Activators / Q000097:blood; D015842:Serine Proteinase Inhibitors / Q000097:blood*; D010959:Tissue Plasminogen Activator / Q000097:blood
D017395:Plasminogen Activator Inhibitor 1; D015842:Serine Proteinase Inhibitors; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator