title: Identification, cDNA cloning, and targeted deletion of p70, a novel, ubiquitously expressed SH3 domain-containing protein.
abstract: In a screen for proteins that interact with Jak2, we identified a previously uncharacterized 70-kDa protein and cloned the corresponding cDNA. The predicated sequence indicates that p70 contains an SH3 domain and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. p70 transcripts were found in all tissues examined. Similarly, when an antibody raised against a C-terminal peptide to analyze p70 protein expression was used, all murine tissues examined were found to express p70. To investigate the in vivo role of p70, we generated a p70-deficient mouse strain. Mice lacking p70 are viable, develop normally, and do not display any obvious abnormalities. No differences were detected in various hematological parameters, including bone marrow colony-forming ability, in response to cytokines that utilize Jak2. In addition, no impairment in B- and T-cell development and proliferative ability was detected.
authors.affiliation: Department of Biochemistry, St. Jude Children's Research Hospital, and University of Tennessee Health Science Center, Memphis, Tennessee 38105, USA.
authors.forename: Nick
authors.initials: N
authors.lastname: Carpino
authors.forename: Ryuji
authors.initials: R
authors.lastname: Kobayashi
authors.forename: Heesuk
authors.initials: H
authors.lastname: Zang
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Shiann-Tarrng
authors.initials: ST
authors.lastname: Jou
authors.forename: Jian
authors.initials: J
authors.lastname: Feng
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nakajima
authors.forename: James N
authors.initials: JN
authors.lastname: Ihle
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology; D019556:COS Cells; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D002455:Cell Division; D002460:Cell Line; D002469:Cell Separation; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005434:Flow Cytometry; D053614:Janus Kinase 2; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010455:Peptides / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011505:Protein-Tyrosine Kinases / Q000737:chemistry* / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000502:physiology; D014018:Tissue Distribution; D014162:Transfection; D014407:Tumor Cells, Cultured; D018909:src Homology Domains
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D018076:DNA, Complementary; D010455:Peptides; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C467906:p70 Jak2-interacting protein, mouse; D011505:Protein-Tyrosine Kinases; C507926:Jak2 protein, mouse; D053614:Janus Kinase 2