Analysis of the polymorphic CAG repeat length in the androgen receptor gene in patients with testicular germ cell cancer.
Changes in the length of a polymorphic trinucleotide (CAG) repeat in the androgen receptor (AR) gene, which may lead to altered transactivation of the AR gene, have been implicated to play a role in the pathogenesis of several forms of endocrine cancer and certain reproductive disorders. Subjects with reproductive disorders that are associated with a relative deficiency of androgen function carry an increased risk for testicular cancer, therefore we have examined the (CAG)n in the AR gene in DNA isolated from peripheral blood cells of 102 patients diagnosed with testicular germ cell neoplasia and compared them with a control group of 110 healthy men with proven fertility. All patients and control subjects underwent comprehensive andrological examination that included reproductive hormone profiles and the analysis of the (CAG)n in the AR gene that was done by means of PCR and DNA sequencing. There was no difference in the distribution of (CAG)n between the subjects and controls, no association of (CAG)n and the tumor type and no association with severity of the disease. We conclude that the high risk of testicular germ cell cancer in the Danish population is not associated with the (CAG)n polymorphism in the AR gene.
Rajpert-De Meyts
Ewa
E
Department of Growth and Reproduction, Copenhagen University Hospital, Section GR-5064, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark. erm@rh.dk
Leffers
Henrik
H
Daugaard
Gedske
G
Andersen
Charlotte B
CB
Petersen
Peter M
PM
Hinrichsen
Jane
J
Pedersen
Lene G
LG
Skakkebaek
Niels E
NE
D000328:Adult; D018237:Germinoma / Q000235:genetics*; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D011944:Receptors, Androgen / Q000235:genetics*; D013736:Testicular Neoplasms / Q000235:genetics*; D018911:Trinucleotide Repeats
D011944:Receptors, Androgen