title: Global analysis of Brucella melitensis proteomes.
abstract: Brucella melitensis is a facultative, intracellular, gram-negative cocco-bacillus that causes Malta fever in humans and brucellosis in animals. There are at least six species in the genus, and the disease is classified as zoonotic because several species infect humans. Using 2-D gel electrophoresis and mass spectrometry, we have initiated (i) a comprehensive mapping and identification of all the expressed proteins of B. melitensis virulent strain 16M, and (ii) a comparative study of its proteome with the attentuated vaccinal strain Rev 1. Comprehensive proteome maps of all six Brucella species will be generated in order to obtain vital information for vaccine development, identification of pathogenicity islands, and establishment of host specificity and evolutionary relatedness.
authors.affiliation: Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510, USA.
authors.forename: Cesar V
authors.initials: CV
authors.lastname: Mujer
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Wagner
authors.forename: Michel
authors.initials: M
authors.lastname: Eschenbrenner
authors.forename: Troy
authors.initials: T
authors.lastname: Horn
authors.forename: Jo Ann
authors.initials: JA
authors.lastname: Kraycer
authors.forename: Rajendra
authors.initials: R
authors.lastname: Redkar
authors.forename: Sue
authors.initials: S
authors.lastname: Hagius
authors.forename: Philip
authors.initials: P
authors.lastname: Elzer
authors.forename: Vito G
authors.initials: VG
authors.lastname: Delvecchio
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001428:Bacterial Vaccines; D002002:Brucella / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D002004:Brucella Vaccine; D017347:Brucella melitensis / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D002006:Brucellosis / Q000517:prevention & control / Q000662:veterinary; D002874:Chromosome Mapping / Q000662:veterinary; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D020543:Proteome / Q000032:analysis*; D013045:Species Specificity; D014774:Virulence; D015047:Zoonoses
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002004:Brucella Vaccine; D020543:Proteome