title: Direct visualization by confocal fluorescent microscopy of the permeation of myristoylated peptides through the cell membrane.
abstract: To study the permeability through the cellular membrane of synthetic peptides containing an hydrophobic moiety, we used a 13-mer myristoylated peptide labeled with a N-terminal fluorescent probe. After 2 h of incubation, the subcellular distribution was analyzed in intact chromaffin cells by confocal fluorescent microscopy. Our results demonstrate that myristoylated peptides diffuse into intact cells, showing an heterogeneous distribution, but they do not reach the cellular nucleous, at least during the time range used.
authors.affiliation: Instituto de Bioingenieria, Universidad Miguel Hernandez, Alicante, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Enseñat-Waser
authors.forename: Franz
authors.initials: F
authors.lastname: Martin
authors.forename: Fernando
authors.initials: F
authors.lastname: Barahona
authors.forename: Jesús
authors.initials: J
authors.lastname: Vazquez
authors.forename: Bernat
authors.initials: B
authors.lastname: Soria
authors.forename: Juan A
authors.initials: JA
authors.lastname: Reig
mesh_terms: D000215:Acylation; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D019439:Chromaffin Cells / Q000378:metabolism* / Q000648:ultrastructure; D004058:Diffusion; D005456:Fluorescent Dyes / Q000032:analysis; D057927:Hydrophobic and Hydrophilic Interactions; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009226:Myristates / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D013347:Subcellular Fractions / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D009226:Myristates; D010455:Peptides