Expression and role of phosphodiesterase 3 in human squamous cell carcinoma KB cells.
Phosphodiesterase (PDE) 3s have been characterized in human squamous cell carcinoma KB cells. PDE3 activity was detected in homogenates of KB cells. PDE3A and 3B mRNAs were detected by RT-PCR in RNA from KB cells; the nucleotide sequences of the fragments were identical to those of human PDE3A and 3B. Immunoblotting with anti-PDE3 antibodies detected both PDE3A- and 3B-immunoreactive proteins in KB cells. The PDE3-specific inhibitor, cilostamide, inhibited the proliferation of KB cells. Our results indicate that PDE3s may be important regulators of the growth of KB cells. Therefore, PDE3 inhibitors may be potential new drugs for antiproliferative therapies in squamous cell carcinoma in the head and neck.
Shimizu
Kasumi
K
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Mie University, Mie 514-8507, Japan. skasumi@clin.medic.mie-u.ac.jp
Murata
Taku
T
Okumura
Kenya
K
Manganiello
Vincent C
VC
Tagawa
Toshiro
T
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000235:genetics / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D006801:Humans; D007624:KB Cells / Q000201:enzymology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; C517287:PDE3A protein, human