title: Porcine-specific hemoglobin saturation measurements.
abstract: The determination of O(2) consumption by using arteriovenous O(2) content differences is dependent on accurate oxyhemoglobin saturation measurements. Because swine are a common experimental species, we describe the validation of CO-oximeter for porcine-specific oxyhemoglobin saturation. After developing a nonlinear mathematical model of the porcine oxyhemoglobin saturation curve, we made 366 porcine oxyhemoglobin saturation determinations with a calibrated blood-gas analyzer and a porcine-specific CO-oximeter. There was a high degree of correlation with minimal variability (r(2) = 0.99, SE of the estimate = 5.2%) between the mathematical model and the porcine-specific CO-oximeter measurements. Bland-Altman comparison showed that the CO-oximeter measurements were biased slightly lower (-0.4 vol%), and the limits of agreement (+/-2 SD) were 0.7 and -1.5 vol%. This is in contrast to a 10-20 vol% error if human-specific methods were used. The results show excellent agreement between the nonlinear model and CO-oximeter for porcine-specific oxyhemoglobin saturation measurements. In contrast, comparison of the porcine-specific oxyhemoglobin saturations with saturations obtained by using human methods highlights the necessity of species-specific measurement methodology.
authors.affiliation: Department of Anesthesiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Serianni
authors.forename: Jed
authors.initials: J
authors.lastname: Barash
authors.forename: Timothy
authors.initials: T
authors.lastname: Bentley
authors.forename: Pushpa
authors.initials: P
authors.lastname: Sharma
authors.forename: John L
authors.initials: JL
authors.lastname: Fontana
authors.forename: Darin
authors.initials: D
authors.lastname: Via
authors.forename: Jochen
authors.initials: J
authors.lastname: Duhm
authors.forename: Rolf
authors.initials: R
authors.lastname: Bunger
authors.forename: Paul D
authors.initials: PD
authors.lastname: Mongan
mesh_terms: D000818:Animals; D006801:Humans; D008954:Models, Biological; D017711:Nonlinear Dynamics; D010092:Oximetry; D010100:Oxygen / Q000097:blood; D010108:Oxyhemoglobins / Q000032:analysis*; D010313:Partial Pressure; D013552:Swine
chemical_list: D010108:Oxyhemoglobins; D010100:Oxygen