title: Bronchoalveolar lavage fluid protein composition in patients with sarcoidosis and idiopathic pulmonary fibrosis: a two-dimensional electrophoretic study.
abstract: We used two-dimensional (2-D) electrophoresis to analyze the protein composition of fluid recovered by bronchoalveolar lavage (BALF) from patients with sarcoidosis and idiopathic pulmonary fibrosis, two forms of interstitial lung disease with different cellular composition and cytokine profile in BALF. They are also characterized by different pathogenesis and clinical evolution, idiopathic pulmonary fibrosis being less favorable than sarcoidosis due to rapidly progressive pulmonary fibrosis. Thirty-eight proteins or protein fragments, never previously assigned in BALF samples, were identified by various methods including mass fingerprinting of tryptic digests. Comparison of the BALF protein maps of the two groups of patients showed 32 spots with statistically significant disease-related variations in relative abundance. In sarcoidosis we found an increase in the amount of several plasma proteins, while in idiopathic pulmonary fibrosis we observed a statistically significant increase in low-molecular-weight proteins, many of which are involved in inflammatory processes (such as MIF and calgranulin) or antioxidant response (such as antioxidant peroxysomal enzyme and thioredoxin peroxidase 2). 2-D electrophoresis allowed us to identify new BALF proteins and to characterize protein composition in patients with sarcoidosis and idiophatic pulmonary fibrosis. Comparison of the gels of the two diseases showed that they differ in BALF protein profiles as they do in type of immune response.
authors.affiliation: Department of Molecular Biology, University of Siena, Italy. magi@unisi.it
authors.forename: Barbara
authors.initials: B
authors.lastname: Magi
authors.forename: Luca
authors.initials: L
authors.lastname: Bini
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Perari
authors.forename: Antonella
authors.initials: A
authors.lastname: Fossi
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Sanchez
authors.forename: Denis
authors.initials: D
authors.lastname: Hochstrasser
authors.forename: Salvo
authors.initials: S
authors.lastname: Paesano
authors.forename: Roberto
authors.initials: R
authors.lastname: Raggiaschi
authors.forename: Annalisa
authors.initials: A
authors.lastname: Santucci
authors.forename: Vitaliano
authors.initials: V
authors.lastname: Pallini
authors.forename: Paola
authors.initials: P
authors.lastname: Rottoli
mesh_terms: D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000032:analysis*; D011658:Pulmonary Fibrosis / Q000378:metabolism*; D012507:Sarcoidosis / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D011506:Proteins