Dietary bile acids inhibit potentially elemental diet-induced small intestinal atrophy in rats.
The mechanism responsible for elemental diet (ED)-induced small intestinal atrophy is still unknown. However, it is possible that bile acids in the gut lumen influence this process. The aim of this study was to evaluate the effects of oral bile acid administration during ED feeding. Specific pathogen-free male Sprague-Dawley rats, 10 weeks old, were fed an ED only, ED plus 0.1% (w/w) hyocholic acid, or ED plus 0.1% (w/w) hyodeoxycholic acid ad libitum for 4 weeks. The control rats were fed standard chow ad libitum for 4 weeks. After 4 weeks, the wet weight and whole length of the small intestine, and the mucosal diamine oxidase (DAO) and alkaline phosphatase (ALP) activities were measured. Microscopic histological observation was also performed. ED feeding induced atrophy and elevations in the mucosal DAO and ALP activities in the small intestine. Hyocholic acid and hyodeoxycholic acid administration both tended to inhibit these alterations. In conclusion, ED feeding induced atrophy and elevations in the mucosal DAO and ALP activities in the small intestine. Oral bile acid administration may prevent this atrophy and the elevations in mucosal DAO and ALP activities, which may lead to new therapeutic strategies in patients managed with ED.
Araki
Yoshio
Y
Ace Bio Product Co, Tokyo, Japan. bileacidaraki@hotmail.com
Andoh
Akira
A
Sasaki
Akira
A
Shimada
Mitsue
M
Bamba
Shigeki
S
Fujino
Sanae
S
Fujiyama
Yoshihide
Y
D000284:Administration, Oral; D000469:Alkaline Phosphatase / Q000378:metabolism; D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D000818:Animals; D001284:Atrophy; D001647:Bile Acids and Salts / Q000008:administration & dosage* / Q000378:metabolism; D005526:Food, Formulated / Q000009:adverse effects*; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000178:diet therapy / Q000188:drug therapy; D007421:Intestine, Small / Q000187:drug effects* / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D001647:Bile Acids and Salts; D006631:Amine Oxidase (Copper-Containing); D000469:Alkaline Phosphatase