title: Dynamic changes of matrix metalloproteinases in rat liver during the development of diethylnitrosamine-induced hepatocarcinoma.
abstract: OBJECTIVE
  To study the dynamic changes of matrix metalloproteinases (MMPs) in the liver of rat during experimental hepatocarcinogenesis.
  
  
  METHODS
  Immunohistochemistry, gelatin zymography, and reverse transcriptase-PCR were used for detection of latent and active forms and mRNA of MMPs in each phases of carcinogenic stages.
  
  
  RESULTS
  MMPs expression was detected in normal and cirrhotic liver, which was most obvious in the cancer cells after the development of hepatic carcinoma. Normal tissue showed only low levels of MMPs expression, which kept increasing in the course of hepatocarcinogenesis, as was also the case with the corresponding mRNA.
  
  
  CONCLUSIONS
  Transcription and expression of MMPs keep increasing throughout the the process of hepatocarcinogenesis.
authors.affiliation: Department of Organ Transplantation, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ze-Sheng
authors.initials: ZS
authors.lastname: Jiang
authors.forename: Shi-Gang
authors.initials: SG
authors.lastname: Fang
authors.forename: Er-Wei
authors.initials: EW
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Bao-Hua
authors.initials: BH
authors.lastname: Chen
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000009:adverse effects; D004052:Diethylnitrosamine / Q000009:adverse effects; D007150:Immunohistochemistry / Q000379:methods; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D012333:RNA, Messenger; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002273:Carcinogens; D012333:RNA, Messenger; D004052:Diethylnitrosamine; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9