IFNgamma production in peripheral blood of early Lyme disease patients to hLFAalphaL (aa326-345).
BACKGROUND
It has been proposed that outer surface protein A (OspA) of Borrelia burgdorferi sensu stricto contains a T helper 1 (Th1) cell epitope that could play a role in an autoimmune response to hLFA1.


METHODS
We used two peptides, hLFAalphaL (aa326-345) and Borrelia burgdorferi OspAB31 (aa164-183), as stimulating antigens to measure Th1 proinflammatory IFNgamma cytokine production in peripheral blood of Lyme disease patients presenting with EM without history of arthritis, as well as in peripheral blood of healthy individuals.


RESULTS
IFNgamma responses to hLFA1 peptide were observed in 11 of 19 Lyme disease patients and in 3 of 15 healthy controls. In contrast, only 2 of 19 of the Lyme disease patients and none of the controls responded to the homologous OspAB31 peptide.


CONCLUSIONS
IFNgamma was produced in response to stimulation with peptide hLFAalphaL (aa326-345) in peripheral blood of 58% of patients with early Lyme disease without signs of arthritis, as well as in peripheral blood of 20% of healthy individuals, but not in response to stimulation with the homologous OspAB31 (aa164-183) peptide (p < 0.05). Our results suggest that reactivity to the hLFA1 peptide in peripheral blood may be the result of T cell degeneracy.
Gomes-Solecki
Maria J C
MJ
Brook Biotechnologies, Inc., Stony Brook, NY 11790, USA. mgomessoleck@notes.cc.sunysb.edu
Wormser
Gary P
GP
Dattwyler
Raymond J
RJ