Maximal forced expiratory maneuver to measure airway obstruction in allergen challenged mice.
Our purpose was to develop a method of using a maximal forced expiratory maneuver (MFEM) for the study of bronchoconstriction and bronchial hyperreactivity (BHR) induced in mice by ovalbumin (OA) inhalation challenge. Eight mice (group I) were sensitized and then provocated with OA. Pulmonary function testing (PFT) at baseline and after varying doses of acetylcholine challenge was performed. Eight weight-matched normal mice served as controls (group II). Pulmonary functions include MFEM, dynamic respiratory system compliance (Crs) and respiratory system resistance (Rrs). The results showed that mice treated with OA had worse PFTs than normal controls, characterized by lower MFEF 50%, FEV0.1 and Crs but higher Rrs. The OA-sensitized mice also had more severe bronchoconstriction in response to acetylcholine, characterized by greater decreases in MFEF 50%, FEV0.1 and Crs but a higher Rrs than the controls. There was a good correlation between PD20MFEF50%Ach and PD20FEV0.1Ach with PD20CrsAch and PD20RrsAch. In conclusion, the MFEM can be used to evaluate airway obstruction and BHR induced in mice by allergen challenge.
Lin
Ching-Chi
CC
Chest Division, Department of Internal Medicine, Mackay Memorial Hospital, 92. Sec. 2, Chung Shan North Road, Taipei, Taiwan. cclin@ms2.mmh.org.tw
Chang
Ching-Fen
CF
Liaw
Shwu-Fang
SF
Lin
Ching-Yuang
CY
D000402:Airway Obstruction / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D000485:Allergens / Q000008:administration & dosage*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D016084:Bronchoconstriction / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D008168:Lung / Q000473:pathology; D008297:Male; D008448:Maximal Expiratory Flow Rate / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D012129:Respiratory Function Tests / Q000379:methods
D000485:Allergens