title: Genetic alterations in adenoma-carcinoma sequencing of intraductal papillary-mucinous neoplasm of the pancreas.
abstract: We investigated the adenoma-carcinoma sequence in intraductal papillary-mucinous neoplasm from the aspect of genetic changes. The formalin-fixed paraffin-embedded tumors and surrounding normal pancreatic tissues from patients with 16 intraductal papillary-mucinous adenoma of the pancreas (IPMA) and 10 intraductal papillary-mucinous carcinoma of the pancreas (IPMC) were provided for DNA extraction after microdissection. SSCP-DNA sequencing analysis demonstrated K-ras mutations at codon 12 in 75% of IPMA and 70% of IPMC, while those at codon 13 were observed neither in IPMA nor IPMC. There were no characteristic K-ras mutation types in IPMA and IPMC and no significant differences in incidence of K-ras mutations between the two categories. The frequencies of p53 mutations analyzed by SSCP-DNA sequencing were not high in IPMA (18.8%) and IPMC (30%), showing no significant difference between them. LOHs of APC in IPMA and IPMC were infrequent (6.3 and 20%, respectively) and showed no significant difference in incidence between the two categories. The LOH frequencies of DCC in IPMA and IPMC were 31.3 and 40%, respectively, and were not statistically different from each other. Taken together, genetic changes such as K-ras, p53, APC and DCC mutations may not be associated with adenoma-carcinoma sequence in intraductal papillary-mucinous neoplasm of pancreas.
authors.affiliation: Department of Surgical Oncology (First Department of Surgery), Osaka City University Graduate Shool of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka 545-8585, Japan. bunzo@med.osaka-cu.ac.jp
authors.forename: Bunzo
authors.initials: B
authors.lastname: Nakata
authors.forename: Masakazu
authors.initials: M
authors.lastname: Yashiro
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Nishioka
authors.forename: Makoto
authors.initials: M
authors.lastname: Aya
authors.forename: Shinobu
authors.initials: S
authors.lastname: Yamada
authors.forename: Chiemi
authors.initials: C
authors.lastname: Takenaka
authors.forename: Masaichi
authors.initials: M
authors.lastname: Ohira
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Hiroji
authors.initials: H
authors.lastname: Nishino
authors.forename: Kenichi
authors.initials: K
authors.lastname: Wakasa
authors.forename: Kosei
authors.initials: K
authors.lastname: Hirakawa
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000235:genetics*; D000236:Adenoma / Q000235:genetics*; D000368:Aged; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics*; D002291:Carcinoma, Papillary / Q000235:genetics*; D005260:Female; D017491:Genes, APC; D016163:Genes, DCC; D016158:Genes, p53; D011905:Genes, ras; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010190:Pancreatic Neoplasms / Q000235:genetics*