title: Production and stability of 3-hydroxypropionaldehyde in Lactobacillus reuteri.
abstract: 3-Hydroxypropionaldehyde (3-HPA) is considered as a potent antimicrobial substance. Exploration of its application as a food preservative or as a therapeutic auxiliary agent has been documented in the literature. In the present work, factors that may impact on 3-HPA accumulation by Lactobacillus reuteri and on the stability of 3-HPA were investigated. Three media - H(2)O, milk and MRS broth - were chosen as test systems. Data indicated that 3-HPA accumulation in resting cells of L. reuteri in a two-step fermentation is greatly affected by temperature, pH, cell age and biomass as well as components in the test system. Within 2 h of incubation, 170 mM 3-HPA could be produced with a cell dry weight of 30 g/l, representing 85% of the glycerol supplied (200 mM) in H(2)O. The presence of glycerol during cell growth increased the productivity of 3-HPA by resting cells. In general, 3-HPA is much more stable in H(2)O than in milk and MRS. Factors that enhanced accumulation of 3-HPA did not simply show the same positive impact on the stability of 3-HPA. Thus, for defined applications, factors affecting production and stability of 3-HPA should be evaluated separately.
authors.affiliation: Laboratory of Dairy Science, Institute of Food Science and Nutrition, Swiss Federal Institute of Technology, 8092 Zurich, Switzerland. qiaoqian.luethi-peng@ilw.agrl.ethz.ch
authors.forename: Q
authors.initials: Q
authors.lastname: Lüthi-Peng
authors.forename: S
authors.initials: S
authors.lastname: Schärer
authors.forename: Z
authors.initials: Z
authors.lastname: Puhan
mesh_terms: D000447:Aldehydes; D005285:Fermentation; D005985:Glyceraldehyde / Q000031:analogs & derivatives* / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D007778:Lactobacillus / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D011407:Propane
chemical_list: D000447:Aldehydes; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; D005990:Glycerol; D011407:Propane