Low dose of resveratrol enhanced immune response of mice.
OBJECTIVE
To study the immune modulating effect of low dose of resveratrol.


METHODS
Concanavalin A (ConA) and Staphylococcus aureus Cowan (Sac) were used to induce the activation of T lymphocyte and antigen presenting cell and cytokine production. [3H]-Thymidine incorporation was used to evaluate the proliferation of lymphocyte. Cytokine production was detected by ELISA method. Dinitrofluorobenzene (DNFB) was used to induce mice delayed type hypersensitivity (DTH, delayed hypersensitivity) response and ear swelling was used as an evaluating indicator. Changes of lymphocyte subtypes were detected by flow cytometry.


RESULTS
Resveratrol (0.75-6 micromol/L) concentration-dependently promoted lymphocyte proliferation and IL-2 production induced by ConA. Sac induced IL-12 and IFN-gamma (interferon type II) production were also concentration-dependently enhanced by resveratrol, while IL-10 production was inhibited. Resveratrol (4 mg/kg, ig) promoted DTH response of mouse, which was suppressed by ethanol (16 %, w/v) consumption. Resveratrol treatment had no significant influence on lymphocyte subtypes in mice, however it could reverse the suppressive effect of ethanol both on macrophage percentage and on macrophage MHC-II molecule expression.


CONCLUSIONS
Low dose resveratrol enhanced cell-mediate immune response. Promoting Th1 cytokine production and influencing on macrophage function might be its mechanisms.
Feng
Yong-Hong
YH
Lab of Immunopharmacology, Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Zhou
Wen-Liang
WL
Wu
Qing-Li
QL
Li
Xiao-Yu
XY
Zhao
Wei-Min
WM
Zou
Jian-Ping
JP
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D004139:Dinitrofluorobenzene; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000276:immunology; D007109:Immunity / Q000187:drug effects; D007371:Interferon-gamma / Q000276:immunology*; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D000077185:Resveratrol; D013154:Spleen / Q000166:cytology; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology
D000276:Adjuvants, Immunologic; D007376:Interleukin-2; D013267:Stilbenes; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma; D004139:Dinitrofluorobenzene; D000077185:Resveratrol