title: Prolactin regulation of the pendrin-iodide transporter in the mammary gland.
abstract: Iodide is an essential constituent of milk that is present in concentrations more than an order of magnitude higher than in the maternal plasma. Earlier, a sodium-iodide symporter was identified in the mammary gland; this transporter is presumed to take iodide from the maternal plasma into the alveolar epithelial cells of the mammary gland. We now report the existence of a second iodide transporter, pendrin, which is also essential for iodide accumulation in milk. Via Western blotting methods, high levels of the transporter were detected in lactating tissues; lesser amounts were found in tissues from midpregnant and virgin mice. Prolactin, at physiological concentrations, stimulated the expression of the pendrin transporter in cultured mammary tissues taken from 12- to 14-day-pregnant mice. The prolactin effect on iodide uptake into cultured mammary tissues was abolished by pendrin transport inhibitors, including DIDS, furosemide, and probenecid. These studies suggest that the prolactin stimulation of pendrin activity is an essential element in the prolactin stimulation of iodide uptake into milk.
authors.affiliation: Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201-1928, USA. jrillema@med.wayne.edu
authors.forename: James A
authors.initials: JA
authors.lastname: Rillema
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Hill
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics*; D046508:Culture Techniques; D005260:Female; D005665:Furosemide / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D007454:Iodides / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000737:chemistry / Q000378:metabolism*; D026901:Membrane Transport Proteins; D051379:Mice; D011247:Pregnancy; D011339:Probenecid / Q000494:pharmacology; D011388:Prolactin / Q000494:pharmacology*; D000076988:Sulfate Transporters
chemical_list: D002352:Carrier Proteins; D007454:Iodides; D026901:Membrane Transport Proteins; C085609:SLC26A4 protein, human; D000076988:Sulfate Transporters; D005665:Furosemide; D011388:Prolactin; D011339:Probenecid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid