title: Inhibitory effect of Artemisia capillaris on ethanol-induced cytokines (TNF-alpha, IL-1alpha) secretion in Hep G2 cells.
abstract: A human hepatoma cell line, Hep G2 cell, is reliable for the study of alcohol-induced hepatotoxicity. In this study, we investigated the effect of an aqueous extract of Artemisia capillaris Thunb (Compositae) plant (AC) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. AC (0.5-5 microg/mL) inhibited the secretion of EtOH-induced interluekin-1alpha (IL-1alpha) and tumor necrosis factor-alpha (TNF-alpha). AC also inhibited the EtOH-, IL-1alpha-, and TNF-alpha-induced cytotoxicity. Furthermore, we found that AC inhibited the EtOH-induced apoptosis of Hep G2 cells. These results suggest that AC may prevent the EtOH-induced cytotoxicity through inhibition of the apoptosis of Hep G2 cells.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, and KI Co. Ltd., Iksan, Jeonbuk, South Korea.
authors.forename: Hyun-Na
authors.initials: HN
authors.lastname: Koo
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Lee
authors.forename: Nam-Guek
authors.initials: NG
authors.lastname: Kim
authors.forename: Su-Deock
authors.initials: SD
authors.lastname: Choi
authors.forename: Ki-Woong
authors.initials: KW
authors.lastname: Ra
authors.forename: Kang-San
authors.initials: KS
authors.lastname: Kim
authors.forename: Byung-Ki
authors.initials: BK
authors.lastname: Kang
authors.forename: Jeong-Joong
authors.initials: JJ
authors.lastname: Kim
authors.forename: Jeong Guk
authors.initials: JG
authors.lastname: Oh
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D019306:Artemisia; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D008099:Liver / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000431:Ethanol