Liquid chromatography analysis of enrofloxacin and ciprofloxacin in chicken blood spotted on filter-paper disks.
A simple, low-cost, sensitive and selective LC method was developed for the determination of enrofloxacin and ciprofloxacin in chicken blood. The method was applied to whole blood from a chicken using dried blood spots on filter paper disks. The detection limits of enrofloxacin and ciprofloxacin (100 microl of whole blood on a disk) were 0.005 and 0.01 microg/ml, respectively. The whole procedure was verified in intra-laboratory studies (recoveries of both compounds were above 90%), and its applicability was tested with blood from the chicken receiving enrofloxacin in a single oral dose at a level of 10 mg/kg body mass. The method permits the use of a small volume of blood from a chicken and should be useful for pharmacokinetic studies.
Posyniak
Andrzej
A
National Veterinary Institute, Department Pharmacology and Toxicology, Al Partyzantow 57, 24-100 Pulawy, Poland. aposyn@piwet.pulawy.pl
Zmudzki
Jan
J
Niedzielska
Jolanta
J
D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood*; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000097:blood*; D000077422:Enrofloxacin; D024841:Fluoroquinolones; D010209:Paper; D015363:Quinolones / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D000077422:Enrofloxacin; D002939:Ciprofloxacin