Characterization of Lactobacillus plantarum from wine must by PCR species-specific and RAPD-PCR.
OBJECTIVE
Physiological and molecular analysis such as PCR species-specific and randomly amplified polymorphic PCR (RAPD-PCR) have been used for typing of Lactobacillus plantarum strains from typical wine must.


RESULTS
Phenotypic tests such as API 50CH and evaluation of D-L-lactate production from glucose were used to perform a preliminary characterization of lactobacilli. Furthermore, 18 strains of lactobacilli were analyzed by PCR species-specific oligonucleotides based on short sequences of the recA gene.


CONCLUSIONS
Four strains were identified as belonging to the L. plantarum species and were further analysed by RAPD-PCR. The RAPD-PCR profiles were similar in all strains that had positive results for species-specific PCR, suggesting that the four L. plantarum strains were closely related.


CONCLUSIONS
Using PCR species-specific as a preliminary screening test and then RAPD-PCR can be as considered the most reliable method of performing a rapid and correct typing of L. plantarum from wine must.
Spano
G
G
Istituto di Produzioni e Preparazioni Alimentari, Facoltà di Agraria, Università degli Studi di Foggia, Foggia, Italy. lab.biomol.tiscali.it
Beneduce
L
L
Tarantino
D
D
Zapparoli
G
G
Massa
S
S
D001430:Bacteriocins / Q000032:analysis / Q000302:isolation & purification*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis* / Q000145:classification; D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012680:Sensitivity and Specificity; D014920:Wine / Q000382:microbiology*
D001430:Bacteriocins; D002241:Carbohydrates; D017931:DNA Primers; C417164:plantaricin UG1