Investigation of cellular metallic elements in single neurons of human brain tissues.
The purpose of this report is to introduce the potential of synchrotron radiation X-ray fluorescence (SRXRF) spectroscopy, by describing the application of this method to mapping and quantification of metallic elements in neurons from brain tissues affected by Alzheimer's disease (AD). In this study, clear images of Fe, Zn and Ca within certain single neurons were obtained. There was a high degree of correlation between Zn and Ca in the groups of cells with lower and relatively higher levels of Ca. These results demonstrate that further investigation with larger numbers of neurons using SRXRF spectroscopy may contribute to advancing the knowledge of the mechanisms of metal-induced cell degeneration in human brain tissues, including those affected by AD.
Ishihara
Ryoko
R
Department of Psycgiatry, Nagoya University Graduate School of Medicine, Japan. ishiyo@med.nagoya-u.ac.jp
Ide-Ektessabi
Ari
A
Ikeda
Kenji
K
Mizuno
Yutaka
Y
Fujisawa
Shigeyoshi
S
Takeuchi
Tohru
T
Ohta
Tatsuro
T
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D001921:Brain / Q000378:metabolism* / Q000503:physiopathology; D002118:Calcium / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000378:metabolism; D008670:Metals / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D013052:Spectrometry, X-Ray Emission / Q000379:methods*; D017356:Synchrotrons; D015032:Zinc / Q000378:metabolism
D008670:Metals; D007501:Iron; D015032:Zinc; D002118:Calcium