Lung transplantation by continuous perfusion in an experimental auto-transplant animal model.
OBJECTIVE
The aim of this study was to evaluate the preservation of the lung using the cold flushing technique in association with continuous perfusion of the organ during static hypothermic storage.


METHODS
In the first phase, the hearts and lungs of 5 New Zealand rabbits were removed three hours after establishing brain death. The left lungs were each conserved in 200 ml of low-potassium UW solution at 10 degrees C for 3 hours of cold ischemia (control group I). The right lungs were also placed in cold storage but were perfused continuously for three hours with low-potassium UW solution at 10 degrees C (group II). In the second phase, ten rabbits underwent a right lung auto-transplant. Lungs were conserved using two techniques. Histoenzymatic and pathological tests were performed: lung function was evaluated.


RESULTS
In the first phase the histopathological examination carried out at the end of storage revealed fewer ischemic alterations in the second group compared to the first. In the second phase a significant hypoxia was observed in group I when both lungs and the right lung only were perfused. The histopathological examination revealed ischaemia/reperfusion lesions in both groups though mainly in group I and a good level of ATPase activity in group II though these results were not significant.


CONCLUSIONS
Cold flushing of the pulmonary artery and continuous perfusion during static hypothermic storage appears to guarantee a better partial arterial pressure of oxygen in this model of auto-transplant compared to the classical cold storage method.
Divisi
D
D
Department of Thoracic Surgery, University of L'Aquila, Italy. duiliodivisi@vizgilio.it
Montagna
P
P
Jegaden
O
O
Ferrera
R
R
Frika
R
R
Santé
P
P
Crisci
R
R
Mikaeloff
P
P
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003080:Cold Temperature; D005978:Glutathione; D007328:Insulin; D016040:Lung Transplantation; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010477:Perfusion / Q000379:methods; D011817:Rabbits; D011887:Raffinose
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose