title: Relationship between adenosine deaminase activity and cytokine-secreting T cells in normal pregnancy.
abstract: OBJECTIVE
  To evaluate the relationship between plasma adenosine deaminase activity and the proportion of cytokine-secreting T cells as causes of changes in adenosine deaminase activity in normal pregnancy.
  
  
  METHODS
  Plasma adenosine deaminase activity and the proportions of cytokine-secreting T cells were measured in the peripheral blood of 26 nonpregnant and normal pregnant women in the third trimester. The proportion of CD4-positive T cells secreting interferon-gamma derived from T helper 1 cells, and interleukin-4 derived from T helper 2 cells, were analyzed by flow cytometry. The ratio of interferon-gamma-secreting cells to interleukin-4-secreting cells was taken as the T helper 1/T helper 2 ratio.
  
  
  RESULTS
  Mean plasma adenosine deaminase activity in normal pregnant women, averaged, was significantly lower than that in nonpregnant women (10.3 +/- 0.6 U/L versus 13.8 +/- 0.5 U/L, P <.05). In normal pregnant women, the proportion of interferon-gamma-secreting cells was significantly lower than that in nonpregnant women (20.5% +/- 1.0% versus 24.7% +/- 1.2%, P <.05), but the proportion of interleukin-4-secreting cells did not differ from that of nonpregnant women. Consequently, the T helper 1/T helper 2 ratios were significantly decreased during normal pregnancy. A significant correlation was found between adenosine deaminase activity and the proportion of interferon-gamma-secreting cells (r =.54, P <.05).
  
  
  CONCLUSIONS
  Decreased plasma adenosine deaminase activity in normal pregnant women may be in part associated with changes in the immunological status, especially the decrease of the proportion of interferon-gamma-secreting cells.
authors.affiliation: Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan. yoshi-1@nms.ac.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yoneyama
authors.forename: Rintaro
authors.initials: R
authors.lastname: Sawa
authors.forename: Shunji
authors.initials: S
authors.lastname: Suzuki
authors.forename: Koichi
authors.initials: K
authors.lastname: Yoneyama
authors.forename: Daisuke
authors.initials: D
authors.lastname: Doi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Araki
mesh_terms: D000243:Adenosine Deaminase / Q000097:blood / Q000378:metabolism*; D000328:Adult; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood / Q000378:metabolism; D015847:Interleukin-4 / Q000097:blood / Q000378:metabolism; D011247:Pregnancy / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D016207:Cytokines; D015847:Interleukin-4; D007371:Interferon-gamma; D000243:Adenosine Deaminase