title: Association of Pasteurella multocida toxin with vimentin.
abstract: To help understand the molecular mechanisms of Pasteurella multocida toxin (PMT) action, we searched for a cellular protein interacting with PMT. The ligand overlay assay revealed a 60-kDa cellular protein that binds to a region from the 840th to 985th amino acids of the toxin. This protein was identified as vimentin by peptide mass fingerprinting. The N-terminal head domain of vimentin was further found to be responsible for the binding to the toxin.
authors.affiliation: Department of Bacterial Toxinology, Research Institute for Microbial Diseases. Research Center for Structural and Functional Proteomics, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shime
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ohnishi
authors.forename: Kaori
authors.initials: K
authors.lastname: Nagao
authors.forename: Kiyomasa
authors.initials: K
authors.lastname: Oka
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Takao
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Horiguchi
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D016979:Pasteurella multocida / Q000472:pathogenicity*; D014746:Vimentin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; C062258:Pasteurella multocida toxin; D014746:Vimentin