Determination of L-sesamin and L-asarinin in Zanthoxylum(Roxb.) DC. by high performance liquid chromatography.
OBJECTIVE
To establish a method for determining the content of L-sesamin and L-asarinin indifferent parts of Zanthoxylum(Roxb.)DC.


METHODS
High-performance liquid chromatography (HPLC) was utilized for this purpose with the column of HYPERSIL BDS C18 and the mobile phase containing acetonitrile and water (50:50), the detection wavelength being 287 nm.


RESULTS
The average recovery rates for L-sesamin and L-asarinin were 100.13% and 1.61% and their relative standard deviation (RSD) were 101.24% and 1.46% respectively.


CONCLUSIONS
The method can be used for the quality control of Zanthoxylum(Roxb.)DC, and the roots of this herb contains more L-sesamin and L-asarinin than its other parts do.
Zhang
Shou-Yao
SY
Department of Pharmaceutics, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Zhou
Ben-Jie
BJ
Wang
Yan
Y
D002851:Chromatography, High Pressure Liquid; D004149:Dioxoles / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D017705:Lignans / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D032106:Zanthoxylum / Q000737:chemistry*
D004149:Dioxoles; D004365:Drugs, Chinese Herbal; D017705:Lignans; C054125:sesamin