[Influence of mutation in HBV precore region on the expression of HLA-I in HepG2 cells].
OBJECTIVE
To study the influence of host cellular HLA-I molecules expression by HBV precore region mutants.


METHODS
The plasmids of the wild type of HBV precore region and the mutants of A83 and A83/A86 were constructed, and then transected into HepG2 cells. The biological activity of HBV precore gene in the host cells was identified. The expression of HLA-I molecules was detected by flow cytometric analysis.


RESULTS
DNA segments similar with HBV precore gene size and HBeAg were detected by PCR and ELISA, respectively. The fluorescence intensity of HLA-I on host cells was different: wild type being 1.3; A83, 17.6; and A83/A86, 7.3.


CONCLUSIONS
HBV precore gene hot-spot mutants in vitro can increase the expression of HLA-I molecules on host cells.
Chen
Weihong
W
Department of Infectious Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Lin
Yulong
Y
Luo
Kangxian
K
Hou
Jinlin
J
D004279:DNA, Viral; D006680:HLA Antigens / Q000276:immunology*; D056945:Hep G2 Cells; D006513:Hepatitis B e Antigens; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D006680:HLA Antigens; D006513:Hepatitis B e Antigens