Genomic and proteomic analyses reveal multiple homologs of genes encoding enzymes of the methanol:coenzyme M methyltransferase system that are differentially expressed in methanol- and acetate-grown Methanosarcina thermophila.
Each of the genomic sequences of Methanosarcina acetivorans, Methanosarcina mazei, and Methanosarcina thermophila revealed two homologs of mtaA, three homologs of mtaB, and three homologs of mtaC encoding enzymes specific for methanogenesis from methanol. Two-dimensional gel electrophoretic analyses of polypeptides from M. thermophila established that methanol induces the expression of mtaA-1, mtaB-1, mtaB-2, mtaB-3, mtaC-1, mtaC-2, and mtaC-3 whereas mtaB-3 and mtaC-3 are constitutively expressed in acetate-grown cells. The gene product of one of three mttC homologs, encoding trimethylamine-specific methyltransferase I, was detected in methanol- but not acetate-grown M. thermophila. A postulated role for the multiple homologs is discussed.
Ding
Yan-Huai R
YH
Department of Biochemistry and Molecular Biology, Eberly College of Science, The Pennsylvania State University, 205 South Frear Laboratory, University Park, PA 16802-4500, USA.
Zhang
Shi-Ping
SP
Tomb
Jean-Francois
JF
Ferry
James G
JG
D000085:Acetates / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D019848:Gene Expression Regulation, Archaeal; D023281:Genomics; D000432:Methanol / Q000378:metabolism; D017020:Methanosarcina / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D040901:Proteomics; D017386:Sequence Homology, Amino Acid
D000085:Acetates; D008780:Methyltransferases; C036890:methanol-2-mercaptoethanesulfonic acid methyltransferase; D000432:Methanol