title: Mathematical modeling of noise and discovery of genetic expression classes in gliomas.
abstract: The microarray array experimental system generates noisy data that require validation by other experimental methods for measuring gene expression. Here we present an algebraic modeling of noise that extracts expression measurements true to a high degree of confidence. This work profiles the expression of 19 200 cDNAs in 35 human gliomas; the experiments are designed to generate four replicate spots/gene with switching of probes. The validity of the extracted measurements is confirmed by: (1) cluster analysis that generates a molecular classification differentiating glioblastoma from lower-grade tumors and radiation necrosis; (2) By what other investigators have reported in gliomas using paradigms for assaying molecular expression other than gene profiling; and (3) Real-time RT-PCR. The results yield a genetic analysis of gliomas and identify classes of genetic expression that link novel genes to the biology of gliomas.
authors.affiliation: Department of Neurological Sciences, Rush Presbyterian-St. Lukes Medical Center, Chicago, Illinois, IL 60612, USA. hfathall@rush.edu
authors.forename: Hassan M
authors.initials: HM
authors.lastname: Fathallah-Shaykh
authors.forename: Mo
authors.initials: M
authors.lastname: Rigen
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zhao
authors.forename: Kanti
authors.initials: K
authors.lastname: Bansal
authors.forename: Bin
authors.initials: B
authors.lastname: He
authors.forename: Herbert H
authors.initials: HH
authors.lastname: Engelhard
authors.forename: Leonard
authors.initials: L
authors.lastname: Cerullo
authors.forename: Kelvin Von
authors.initials: KV
authors.lastname: Roenn
authors.forename: Richard
authors.initials: R
authors.lastname: Byrne
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Munoz
authors.forename: Gail L
authors.initials: GL
authors.lastname: Rosseau
authors.forename: Roberta
authors.initials: R
authors.lastname: Glick
authors.forename: Terry
authors.initials: T
authors.lastname: Lichtor
authors.forename: Elia
authors.initials: E
authors.lastname: DiSavino
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D005909:Glioblastoma / Q000235:genetics; D005910:Glioma / Q000235:genetics*; D006801:Humans; D008962:Models, Theoretical; D005810:Multigene Family; D020133:Reverse Transcriptase Polymerase Chain Reaction