title: Tech.Sight. Phage display. Affinity selection from biological libraries.
abstract: Phage display is a simple yet powerful technology that is used to rapidly characterize protein-protein interactions from amongst billions of candidates. This widely practiced technique is used to map antibody epitopes, create vaccines and to engineer peptides, antibodies and other proteins as both diagnostic tools and as human therapeutics. We overview the history of phage display and several recent applications.
authors.affiliation: Department of Antibody Technologies, Amgen Inc., Seattle, WA 98101, USA. SmotherJ@amgen.com
authors.forename: James F
authors.initials: JF
authors.lastname: Smothers
authors.forename: Steven
authors.initials: S
authors.lastname: Henikoff
authors.forename: Paul
authors.initials: P
authors.lastname: Carter
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D000915:Antibody Affinity; D001435:Bacteriophages / Q000235:genetics; D030562:Databases, Protein; D018604:Epitope Mapping; D006801:Humans; D036103:Nanotechnology; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012666:Semiconductors
chemical_list: D000906:Antibodies; D019151:Peptide Library; D010455:Peptides; D011506:Proteins; D011993:Recombinant Fusion Proteins