Association of Pasteurella multocida toxin with vimentin.
To help understand the molecular mechanisms of Pasteurella multocida toxin (PMT) action, we searched for a cellular protein interacting with PMT. The ligand overlay assay revealed a 60-kDa cellular protein that binds to a region from the 840th to 985th amino acids of the toxin. This protein was identified as vimentin by peptide mass fingerprinting. The N-terminal head domain of vimentin was further found to be responsible for the binding to the toxin.
Shime
Hiroaki
H
Department of Bacterial Toxinology, Research Institute for Microbial Diseases. Research Center for Structural and Functional Proteomics, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan.
Ohnishi
Takahiro
T
Nagao
Kaori
K
Oka
Kiyomasa
K
Takao
Toshifumi
T
Horiguchi
Yasuhiko
Y
D016475:3T3 Cells; D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D016979:Pasteurella multocida / Q000472:pathogenicity*; D014746:Vimentin / Q000737:chemistry / Q000378:metabolism*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C062258:Pasteurella multocida toxin; D014746:Vimentin