Sequence analysis of three Ehrlichia ruminantium LambdaGEM-11 clones.
Three Lambda GEM11 clones were isolated from a large-insert Ehrlichia ruminantium Welgevonden library. The inserts were amplified, sequenced, and analyzed. A total of 39 827 bp was obtained, and 18 different open reading frames (ORFs) were identified. Long repeats (100-200 kbp) were found in all three sequences. These repeats may play a role in the induction of antigenic variation. Along with a 20-kbp sequence of a clone from the E. ruminantium cosmid library, these sequences are the first large sequences to be yielded by the E. ruminantium genome sequencing project.
Pretorius
Alri
A
Molecular Biology Section, Onderstepoort Veterinary Institute, Pretoria, South Africa. Alri@moon.ovi.ac.za
Collins
N E
NE
Steyn
H C
HC
Allsopp
B A
BA
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000737:chemistry*; D016995:Ehrlichia ruminantium / Q000235:genetics*; D015723:Gene Library; D016680:Genome, Bacterial; D016366:Open Reading Frames / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D017385:Sequence Homology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011994:Recombinant Proteins