title: A synthetic strategy for ON-resin amino acid specific multiple fatty acid acylation of peptides.
abstract: Covalent modification with fatty acids is observed in several proteins that play crucial roles in cellular physiology. In this paper, a convenient method for the generation of multiple fatty acylated synthetic peptides is described. Peptides were synthesized using solid phase procedures with fluorenylmethoxycarbonyl a-amino protected amino acids. Acetamidomethyl protected cysteines were employed. The thiol protecting group was selectively deprotected and acylation was carried out on the resin-bound peptides. The strategy described in this report is applicable to any peptide sequence.
authors.affiliation: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
authors.forename: Avaronnan
authors.initials: A
authors.lastname: Harishchandran
authors.forename: Bhattaram
authors.initials: B
authors.lastname: Pallavi
authors.forename: Ramakrishnan
authors.initials: R
authors.lastname: Nagaraj
mesh_terms: D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism*; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000378:metabolism*; D013058:Mass Spectrometry; D008623:Mercaptoethanol / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000378:metabolism; D012116:Resins, Plant / Q000737:chemistry; D019061:src-Family Kinases / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D010455:Peptides; D012116:Resins, Plant; D008623:Mercaptoethanol; D000217:Acyltransferases; D019061:src-Family Kinases