title: [Application of PCR technique in genetic diagnosis of Duchenne/Becker muscular dystrophy].
abstract: OBJECTIVE
  To study the application of PCR technique in genetic detection of Duchenne/Becker muscular dystrophy (DMD/BMD).
  
  
  METHODS
  A multiple PCR system is established according to the multiple sites of DMD/BMD exon deletion. Under different PCR conditions, multiple exon deletions, single-strand conformation polymorphism, allopolyploid, chain labeling, restriction fragment length polymorphism and microsatellite phenomenon were examined in 23 DMD/BMD patients and 57 suspected carriers of these genes.
  
  
  RESULTS
  Fourteen of the 23 DMD/BMD patients were identified as having gene deletion, with another 2 carried gene duplicates. Forty female relatives of these 23 DMD/BMD patients were diagnosed as carriers of the genes.
  
  
  CONCLUSIONS
  This PCR system can be applied in detecting gene mutation of DMD/BMD, screening the carriers and in appropriate genealogical analysis of the patients with DMD/BMD.
authors.affiliation: Department of Pediatrics, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Liu
authors.forename: Zhi-Chun
authors.initials: ZC
authors.lastname: Feng
authors.forename: Zhen-Wei
authors.initials: ZW
authors.lastname: Fang
mesh_terms: D019937:Diagnostic Techniques and Procedures; D005260:Female; D017353:Gene Deletion; D006801:Humans; D008297:Male; D020388:Muscular Dystrophy, Duchenne / Q000175:diagnosis* / Q000235:genetics; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide