[Enhanced sensitivity of ovarian cell line to cisplatin induced by mifepristone and its mechanism].
OBJECTIVE
To observe the effect of mifepristone in enhancing chemosensitivity of drug-resistant ovarian cancer cell line to cisplatin and investigate its mechanism.


METHODS
Human ovarian cancer cell lines COC1 (DDP-sensitive) and COC1/DDP (DDP-resistant) were incubated with or without mifepristone. The proliferation rate of the cells was determined by methyl thiazolyl tetrazolium (MTT) assay and positive expression of apoptosis-associated proteins Bcl-2 and Bax were observed by means of flow cytometry.


RESULTS
It was observed that mifepristone at the dose of 1.25 micromol/L reversed the resistance of COC1/DDP cells to cisplatin, and Bcl-2 protein expression was deceased from (23.8067+/-0.4382)% to (19.3967+/-0.6866)% (P=0.000) while Bax protein expression increased from (12.75+/-0.2524)% to (25.5967+/-0.8834)% (P=0.000).


CONCLUSIONS
Mifepristone may act to enhance the sensitivity of COC1/DDP cells to cisplatin, possibly through regulating Bcl-2 and Bax protein expressions.
Qin
Tang-Ni
TN
Department of Obstetrics and Gynecology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Wang
Li-Li
LL
D000021:Abortifacient Agents, Steroidal / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D004347:Drug Interactions; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D015735:Mifepristone / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein
D000021:Abortifacient Agents, Steroidal; D000970:Antineoplastic Agents; C494826:BAX protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D015735:Mifepristone; D002945:Cisplatin