title: [Construction and biological activity assessment of core mutants of hepatitis B virus adr subtype].
abstract: OBJECTIVE
  To construct the core mutant (L97 and V60) plasmids of hepatitis B virus (HBV) and assess their biological activity.
  
  
  METHODS
  Site-directed mutagenesis was performed to induce specific core point mutations in HBV adr suhtype 1.2 copy genome plasmid p3.8 II. The plasmids were transfected into HepG2 cells via liposome, and intracellular HBV DNA was analyzed by Southern hybridization, while extracellular HBV antigens (HBsAg and HBeAg) in the culture supernatant were assayed by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Sequence analysis of the constructed mutant plasmids p3.8 L97 and p3.8 V60 demonstrated mutations at 2 189 A C and 2 086 C HBV adr subtype genome core mutant (L97 and V60) plasmids we have constructed possess biological activity.G respectively. These mutant plasmids exhibited HBV DNA replication activity and gene expression in host cells.
  
  
  CONCLUSIONS
  HBV adr subtype genome core mutant (L97 and V60) plasmids we have constructed possess biological activity.
authors.affiliation: Department of Infectious Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Chen
authors.forename: Yu-Long
authors.initials: YL
authors.lastname: Lin
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: Kang-Xian
authors.initials: KX
authors.lastname: Luo
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D017354:Point Mutation; D014407:Tumor Cells, Cultured; D014758:Viral Core Proteins / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics
chemical_list: D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D014758:Viral Core Proteins