title: Molecular cloning and complete cDNA sequence of UBH1 in mouse testis.
abstract: We have identified a full-length mouse UBH1 cDNA, encoding a putative deubiquitinating enzyme, from the testis by RT-PCR using primers prepared from sequences conserved amongst deubiquitinating enzymes. Sequence analysis predicts that the UBH1 cDNA encodes a 355 amino acid polypeptide with the molecular weight of approximately 39 kDa containing the highly conserved Cys, Asp, and His domains characteristic of the ubiquitin-specific processing proteases. Biochemical assay revealed that the mouse UBH1 has deubiquitinating enzyme activity and sequence analysis showed 98.3% amino acid identity with human UBH1.
authors.affiliation: College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute, CHA General Hospital, Seoul, South Korea. baek@cha.ac.kr
authors.forename: Kwang-Hyun
authors.initials: KH
authors.lastname: Baek
authors.forename: Kyu-Hyung
authors.initials: KH
authors.lastname: Park
authors.forename: Yong-Soo
authors.initials: YS
authors.lastname: Kim
authors.forename: Myung-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Hee-Kyung
authors.initials: HK
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D010450:Endopeptidases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013737:Testis / Q000378:metabolism*; D043222:Ubiquitin Thiolesterase
chemical_list: D018076:DNA, Complementary; D010450:Endopeptidases; D043222:Ubiquitin Thiolesterase; C486401:Usp12 protein, mouse