Characterization of a strawberry cDNA clone homologous to calcium-dependent protein kinases that is expressed during fruit ripening and affected by low temperature.
A full-length cDNA clone (FaCDPK1) encoding a calcium-dependent protein kinase (CDPK) has been isolated from a strawberry fruit cDNA library. FaCDPK1 contains the basic features of CDPKs: a catalytic kinase domain linked to a regulatory calmodulin-like domain by a junction sequence that has been shown to act as an autoinhibitory pseudosubstrate. Although the calmodulin-like domain of CDPKs typically contains four EF-hand calcium-binding motifs, FaCDPK1 was predicted to contain only three EF-hand motifs. FaCDPK1 gene expression was observed in roots, stolons, meristems, flowers, and leaves. FaCDPK1 mRNA was not detected in young fruits, but accumulated as fruit turned to white, suggesting a role for this gene in the developing strawberry fruit. In ripe fruit the levels of transcript increased in response to low temperature.
Llop-Tous
Immaculada
I
Departmento de Fisiología y Biodiversidad Molecular, Instituto de Biología Molecular de Barcelona, CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
Domínguez-Puigjaner
Eva
E
Vendrell
Miquel
M
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D031985:Fragaria / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005638:Fruit / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D018076:DNA, Complementary; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases