title: The transcriptional regulator AlgR is essential for Pseudomonas aeruginosa pathogenesis.
abstract: Chronic Pseudomonas aeruginosa lung infection is the major cause of morbidity and mortality in cystic fibrosis (CF) patients. One P. aeruginosa virulence factor unique to CF isolates is overproduction of alginate, phenotypically termed mucoidy. Mucoidy is the result of increased transcription from the algD gene and is activated by the transcriptional regulator AlgR. Mutations in algR result in a nonmucoid phenotype and loss of twitching motility. Additionally, AlgR controls transcription of algC, encoding a dual-function enzyme necessary for both lipopolysaccharide (LPS) and alginate production. Therefore, to determine the effect of algR on P. aeruginosa virulence, an algR mutant was examined for sensitivity to reactive oxygen intermediates, killing by phagocytes, systemic virulence, and the ability to maintain a murine lung infection. We found that P. aeruginosa PAO700 (algR::Gm(r)) was less lethal than PAO1, as tested in an acute septicemia infection mouse model, and was cleared more efficiently in a mouse pneumonia model. Additionally, the algR mutant (PAO700) was more sensitive to hypochlorite. However, PAO700 was more resistant to hydrogen peroxide and killed less readily in an acellular myeloperoxidase assay than PAO1. There was little difference in killing between PAO1 and PAO700 with macrophage-like J774 cells and human polymorhonuclear leukocytes. Two-dimensional gel analysis of P. aeruginosa algR mutant and wild-type protein extracts revealed 47 differentially regulated proteins, suggesting that AlgR plays both a positive role and a negative role in gene expression. Together, these results imply that AlgR is necessary for virulence and regulates genes in addition to the genes associated with alginate and LPS production and pilus function.
authors.affiliation: Department of Microbiology and Immunology, Program in Molecular Pathogenesis and Immunity, Tulane University Health Sciences Center, New Orleans, Louisiana 70112-2699, USA.
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Lizewski
authors.forename: Derek S
authors.initials: DS
authors.lastname: Lundberg
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Schurr
mesh_terms: D000464:Alginates / Q000378:metabolism*; D000818:Animals; D016470:Bacteremia / Q000382:microbiology; D001426:Bacterial Proteins / Q000502:physiology*; D005260:Female; D005949:Glucose Oxidase / Q000494:pharmacology; D006801:Humans; D006997:Hypochlorous Acid / Q000494:pharmacology; D008168:Lung / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009195:Peroxidase / Q000494:pharmacology; D017875:Phosphotransferases (Phosphomutases) / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D015534:Trans-Activators; D014158:Transcription, Genetic; D014774:Virulence
chemical_list: C110681:AlgR protein, Pseudomonas aeruginosa; D000464:Alginates; D001426:Bacterial Proteins; D015534:Trans-Activators; D006997:Hypochlorous Acid; D005949:Glucose Oxidase; D009195:Peroxidase; D017875:Phosphotransferases (Phosphomutases); C052799:phosphomannomutase