title: [Detection of apoptosis with gel eletrophoresis combined with computerized gel analysis system].
abstract: OBJECTIVE
  To explore a new method for detecting cellular apoptosis.
  
  
  METHODS
  Using routine electrophoresis assisted by computerized gel documentation analysis system(GDAS), apoptosis of the macrophages treated with dexamethasone was qualitatively and then quantitatively detected.
  
  
  RESULTS
  Apoptosis occurred in the macrophages treated with dexamethasone, and DNA fragmentation of the apoptotic macrophages was visualized on 1% agarose gel electrophoresis. The apoptosis rates as determined by computerized GDAS were consistent with the results obtained from flow cytometry that showed typical apoptosis peak.
  
  
  CONCLUSIONS
  Agarose gel electrophoresis assisted by computerized GDAS was relatively simple and less costly in qualitative and quantitative detection of apoptosis with precision.
authors.affiliation: Central Laboratory, First Military Medical University, Guangzhou 510515, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.forename: Jin-Ya
authors.initials: JY
authors.lastname: Wu
authors.forename: Xing-Xu
authors.initials: XX
authors.lastname: Huang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Ma
authors.forename: Yong-Yao
authors.initials: YY
authors.lastname: Bao
authors.forename: Jun-Ling
authors.initials: JL
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D051379:Mice
chemical_list: D003907:Dexamethasone