Optimization of media by evolutionary algorithms for production of polyols.
Biotransformation of sucrose-based medium to polyols has been reported for the first time using osmophilic yeast, Hansenula anomala. A new, real coded evolutionary algorithm was developed for optimization of fermentation medium in parallel shake-flask experiments. By iteratively employing the nature-inspired techniques of selection, crossover, and mutation for a fixed number of generations, the algorithm obtains the optimal values of important process variables, namely, inoculum size and sugar, yeast extract, urea, and MgSO4 concentrations. Maximum polyols yield of 76.43% has been achieved. The method is useful for reducing the overall development time to obtain an efficient fermentation process.
Patil
S V
SV
Department of Alcohol Technology, Vasantdada Sugar Institute, Pune, India.
Jayaraman
V K
VK
Kulkarni
B D
BD
D000465:Algorithms; D001711:Biotransformation; D003470:Culture Media; D005285:Fermentation; D008278:Magnesium Sulfate / Q000494:pharmacology; D008957:Models, Genetic; D010843:Pichia / Q000254:growth & development / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology; D014508:Urea / Q000378:metabolism / Q000494:pharmacology
D003470:Culture Media; D011108:Polymers; C024617:polyol; D013395:Sucrose; D008278:Magnesium Sulfate; D014508:Urea