title: [A role of protein kinase C in signal reactions in outer segments of the bovine retinal rods].
abstract: The effect of modulators of protein kinase C activity on Ca2+ translocation in dark-adapted and bleached retinal rod outer segments (ROS) was studied. The activators (1,2-diacyl glycerol and phorbol-12-myristate-13-acetate) and the inhibitor (chelerythrine chloride) of protein kinase C were shown to stimulate and inhibit the ATP-dependent Ca(2+)-uptake in dark-adapted retinal ROS, correspondingly. Apparently, this action is due to the influence of protein kinase C on Ca(2+)-ATPase activity in these vesicular structures. No involvement of modulators of protein kinase C activity on ATP-dependent Ca(2+)-uptake in bleached retinal ROS was found. The influence of protein kinase C on Ca(2+)-release from retinal ROS was observed. It was shown that the activators and inhibitors of protein kinase C increased the efficiency of this process both in dark-adapted and bleached retinal ROS. The mechanisms of action of the protein kinase C activity modulators on the Ca(2+)-uptake and Ca(2+)-release in retinal ROS are discussed.
authors.affiliation: Institute of Photobiology, National Academy of Sciences of Belarus, ul. Akademicheskaya 27, Minsk, 220072 Belarus.
authors.forename: V P
authors.initials: VP
authors.lastname: Emel'ianova
authors.forename: L A
authors.initials: LA
authors.lastname: Baranova
authors.forename: S S
authors.initials: SS
authors.lastname: Siniushkina
authors.forename: I D
authors.initials: ID
authors.lastname: Volotovskiĭ
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000470:Alkaloids; D000818:Animals; D053119:Benzophenanthridines; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D002417:Cattle; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D010617:Phenanthridines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology*; D012374:Rod Cell Outer Segment / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: C011439:1,2-diacylglycerol; D000470:Alkaloids; D053119:Benzophenanthridines; D004075:Diglycerides; D010617:Phenanthridines; D000255:Adenosine Triphosphate; C016299:chelerythrine; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium