title: Residual caries detection using visible fluorescence.
abstract: This study investigated the ability of a new fluorescence method to detect residual caries in vitro. Gross caries was removed from 40 teeth with D2 caries. Samples were excited with violet-blue light and viewed through a 530-nm high-pass filter. Residual caries (orange-red fluorescing dentin) was detected in all samples. Further tooth substance was removed from half of the samples until no residual caries was detectable using the new method. Half of the samples remained untreated. A blinded examiner checked all samples for residual caries using DIAGNOdent, a visual tactile examination, and Caries Detector dye. Presence or absence of residual caries in each sample was determined using a fluorescent nucleic acid stain in conjunction with confocal microscopy. The new method, Visible Fluorescence, had the greatest sensitivity, specificity, percent correct score and predictive values of any of the methods tested. The new method had significantly higher percent correct score than any of the other methods and significantly higher specificity than visual tactile and Caries Detector. It was concluded that Visible Fluorescence is an improvement on the currently available aids for residual caries detection.
authors.affiliation: Department of Preventive and Community Dentistry, Oral Health Research Institute, Indiana University School of Dentistry, Indianapolis, Ind, USA. lennon@med.uni-goettingen.de
authors.forename: A M
authors.initials: AM
authors.lastname: Lennon
authors.forename: W
authors.initials: W
authors.lastname: Buchalla
authors.forename: L
authors.initials: L
authors.lastname: Switalski
authors.forename: G K
authors.initials: GK
authors.lastname: Stookey
mesh_terms: D001641:Bicuspid; D003731:Dental Caries / Q000175:diagnosis*; D003732:Dental Caries Activity Tests; D003804:Dentin / Q000473:pathology; D005453:Fluorescence; D006801:Humans; D007834:Lasers; D018613:Microscopy, Confocal; D008963:Molar; D012680:Sensitivity and Specificity