title: Intracellular calcium activity in isolated bovine adrenal chromaffin cells in the presence and absence of 60 Hz magnetic fields.
abstract: This study examined whether 60 Hz magnetic field (MF) exposure alters intracellular calcium levels ([Ca(2+)](i)) in isolated bovine adrenal chromaffin cells, a classic model of neural responses. [Ca(2+)](i) was monitored by fluorescence video imaging of cells loaded with the calcium indicator fluo-4 during exposures to magnetic flux densities of 0.01, 0.1, 1.0, 1.4, or 2.0 mT. MFs generated by Helmholtz coils constructed from bifilar wire allowed both 60 Hz field and sham exposures. Following a 5 min monitoring period to establish baseline patterns, cells were subjected for 10 min to a 60 Hz MF, sham field or no field. Reference calcium responses and assessment of cell excitability were obtained by the sequential addition of the nicotinic cholinergic receptor agonist dimethylphenylpiperazinium (DMPP) and a depolarizing concentration of KCl. Throughout an 8 day culture period, cells exhibited spontaneous fluctuations in [Ca(2+)](i). Comparisons of the number of cells exhibiting transients, the number and types of calcium transients, as well as the time during monitoring when transients occurred showed no significant differences between MF exposed cells and either sham exposed or nonexposed cells. With respect to the percentage of cells responding to DMPP, differences between 1 and 2 mT exposed cells and both nonexposed and sham exposed cells reached statistical significance during the first day in culture. No statistically significant differences were observed for responses to KCl. In summary, our data indicate that [Ca(2+)](i) in chromaffin cells is unaffected by the specific 60 Hz MF intensities used in this study. On the other hand, plasma membrane nicotinic receptors may be affected in a manner that is important for ligand-receptor interactions.
authors.affiliation: Department of Pharmacology, University of Nevada, Reno, Nevada 89557, USA. gcraviso@unr.edu
authors.forename: Gale L
authors.initials: GL
authors.lastname: Craviso
authors.forename: James
authors.initials: J
authors.lastname: Poss
authors.forename: Christine
authors.initials: C
authors.lastname: Lanctot
authors.forename: Steven S
authors.initials: SS
authors.lastname: Lundback
authors.forename: Indira
authors.initials: I
authors.lastname: Chatterjee
authors.forename: Nelson G
authors.initials: NG
authors.lastname: Publicover
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D000818:Animals; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D004246:Dimethylphenylpiperazinium Iodide / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D011189:Potassium Chloride / Q000494:pharmacology; D012016:Reference Values; D012680:Sensitivity and Specificity
chemical_list: D004246:Dimethylphenylpiperazinium Iodide; D011189:Potassium Chloride; D002118:Calcium