title: [Expression of cre gene in Escherichia coli and bioassay its expression product].
abstract: The Cre recombinase from bacteriophage P1 can recognize specific DNA sequences, cleave DNA at specific target sites, and then ligate it to the cleaved DNA of a second site. In this study, cre gene was cloned into the pGEM-T Easy vector via PCR procedure. Then the cre gene was inserted into an expression vector pET-29a and expressed in E. coli BL21 (DE3). A 38 kD soluble protein was expressed and named CRE. CRE was purified by DEAE-52 chromatography. Bioassay of the partially purified product showed that CRE can cleave the plasmid pGLGFP which contains two loxP site with the same direction.
authors.affiliation: National Key Laboratory of Protein Engineering and Plant Molecular Biology of Peking University, China.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Wang
authors.forename: Zhu-Qiang
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Xiao-Qian
authors.initials: XQ
authors.lastname: Hu
authors.forename: Yuan-Lei
authors.initials: YL
authors.lastname: Hu
authors.forename: Yin
authors.initials: Y
authors.lastname: Gao
authors.forename: Zhong-Ping
authors.initials: ZP
authors.lastname: Lin
mesh_terms: D002848:Chromatography, DEAE-Cellulose; D004926:Escherichia coli / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D049452:Green Fluorescent Proteins; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D008164:Luminescent Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases