title: Mutations in ribosomal protein L16 and in 23S rRNA in Enterococcus strains for which evernimicin MICs differ.
abstract: Mutations in ribosomal protein L16 and in 23S rRNA were investigated in 22 Enterococcus strains of different species and for which the MICs of evernimicin differ (MICs, 0.023 to 16 micro g/ml). Amino acid changes (Arg56His, Ile52Thr, or Arg51His) in protein L16 were found in seven strains, and a nucleotide G2535A mutation in 23S rRNA was found in 1 strain among 13 for which the MICs are > or =1 micro g/ml.
authors.affiliation: Area de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.
authors.forename: Myriam
authors.initials: M
authors.lastname: Zarazaga
authors.forename: Carmen
authors.initials: C
authors.lastname: Tenorio
authors.forename: Rosa
authors.initials: R
authors.lastname: Del Campo
authors.forename: Fernanda
authors.initials: F
authors.lastname: Ruiz-Larrea
authors.forename: Carmen
authors.initials: C
authors.lastname: Torres
mesh_terms: D019943:Amino Acid Substitution; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D012338:RNA, Ribosomal, 23S; D012269:Ribosomal Proteins; C027937:ribosomal protein L16; C001202:evernimicin