Platelet factor 4 fragment induces histamine release from rat peritoneal mast cells.
Platelet factor 4 (PF-4) belongs to a superfamily of low-molecular weight proteins known as chemokines. However, its function has not been fully evaluated. In the present study, we investigated the effect of PF-4 on histamine release from rat peritoneal mast cells by employing its biologically-active carboxyl-terminal fragment, PF-4 (58-70). PF-4 (58-70) stimulated histamine release from mast cells in a dose-dependent manner (10(-8) to 10(-5)M). Histamine release induced by PF-4 (58-70) occurred rapidly (<30s) and was inhibited by extracellular Ca(2+). These results suggest that PF-4 might play a crucial role at the site of inflammation and/or immune response.
Suzuki
Ryujiro
R
Laboratory Medicine, Nagoya University School of Medicine, Nagoya, Japan.
Kimura
Tomoki
T
Kitaichi
Kiyoyuki
K
Tatsumi
Yasuaki
Y
Matsushima
Miyoko
M
Zhao
Ying Lan
YL
Shibata
Eiji
E
Baba
Kenji
K
Hasegawa
Takaaki
T
Takagi
Kenzo
K
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D010978:Platelet Factor 4 / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D010446:Peptide Fragments; D010455:Peptides; D010978:Platelet Factor 4; D002118:Calcium