Molecular imaging of perfusion disturbances in glaucoma.
Ocular ischemia resulting from perfusion disturbances may play a major role in initiation of glaucoma. Possibly secondary to ischemia autoimmunogenic events are activated in glaucoma patients with increased prevalence of systemic autoimmune diseases. The determination of potential molecular markers in blood leukocytes could be useful for early noninvasive diagnostics of glaucoma. Our study using subtractive hybridization showed altered gene expression in leukocytes of glaucoma patients in comparison to age and sex matched healthy subjects. Subtracted genes encoding lymphocyte IgE receptor (Fc epsilon RII/CD23), T cell-specific tyrosine kinase, thromboxan A2 receptor, alkaline phosphatase and Na(+)/K(+)-ATPase are differentially expressed in circulating leukocytes of glaucoma patients. These genes show expression profiles characteristic for adherent leukocytes which could be an important contributor to blood-brain barrier breakdown which has been found in glaucoma patients.
Golubnitschaja
O
O
Department of Radiology, University of Bonn, Germany. Olga.Golubnitschaja@ukb.uni-bonn.de
Wunderlich
K
K
Decker
C
C
Mönkemann
H
H
Schild
H H
HH
Flammer
J
J
D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D001812:Blood-Brain Barrier / Q000502:physiology* / Q000503:physiopathology; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D005901:Glaucoma / Q000503:physiopathology*; D006801:Humans; D006824:Hybridization, Genetic; D007511:Ischemia / Q000378:metabolism*; D007962:Leukocytes / Q000166:cytology / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D017455:Receptors, IgE / Q000235:genetics; D044244:Receptors, Thromboxane A2, Prostaglandin H2 / Q000235:genetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics
D017455:Receptors, IgE; D044244:Receptors, Thromboxane A2, Prostaglandin H2; D011505:Protein-Tyrosine Kinases; C080813:emt protein-tyrosine kinase; D000469:Alkaline Phosphatase; D000254:Sodium-Potassium-Exchanging ATPase