title: Construction of stable anionic liposome-plasmid particles using the heating method: a preliminary investigation.
abstract: Recent advances in liposome technology have resulted in the production of effective drug delivery formulations, although toxicity concerns remain. In order to overcome this problem we prepared anionic liposomes without using any volatile organic solvent or detergent. Liposomes prepared by this heating method (HM-liposomes) were characterised in terms of morphology, stability and DNA incorporation efficiency. Scanning tunnelling microscopy (STM) and optical microscopy were used to study the morphological characteristics and size distribution of HM-liposomes. Microscopic studies revealed formation of spherical bilayered structures with stabilities of at least eight months and also enabled measuring the diameter and the bilayer thickness of the vesicles. Plasmid DNA encapsulation efficiencies of up to 70.3% were determined for HM-liposomes.
authors.affiliation: School of Pharmacy and Chemistry, Liverpool John Moores University, Liverpool, L3 3AF, England, UK. pacmmoza@livjm.ac.uk
authors.forename: M Reza
authors.initials: MR
authors.lastname: Mozafari
authors.forename: Celia J
authors.initials: CJ
authors.lastname: Reed
authors.forename: Christopher
authors.initials: C
authors.lastname: Rostron
authors.forename: Cengiz
authors.initials: C
authors.lastname: Kocum
authors.forename: Erhan
authors.initials: E
authors.lastname: Piskin
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine; D000838:Anions; D002214:Capsules; D002784:Cholesterol; D004337:Drug Carriers; D008081:Liposomes / Q000138:chemical synthesis* / Q000493:pharmacokinetics*; D016252:Microscopy, Scanning Tunneling / Q000379:methods; D010755:Organophosphates; D010957:Plasmids; D014018:Tissue Distribution
chemical_list: D000838:Anions; D002214:Capsules; D004337:Drug Carriers; D008081:Liposomes; D010755:Organophosphates; D015060:1,2-Dipalmitoylphosphatidylcholine; C002290:dicetylphosphate; D002784:Cholesterol