Genotoxic effects of stannous chloride (SnCl2) in K562 cell line.
The toxic effects of SnCl2 in K562 cells were analyzed in this study. This cell line is resistant to reactive oxygen species (ROS) making it suitable to evaluate the impact of SnCl2 in culture either through ROS or by direct toxicity using Trypan blue dye exclusion, comet and flow cytometry assays. An important loss of viability induced by SnCl2 in a dose-response manner was observed in cells treated in Tris-buffered saline (TBS). This necrotic cell death was further confirmed by flow cytometry. On the other hand, there was no loss of viability when cells were treated in rich medium (RPMI). DNA damage was visualized in SnCl2-treated K562 cells in both tested conditions. The data indicate that SnCl2 induces DNA damage and reduces K562 viability. Both actions seem to be correlated with ROS formation and direct linkage to DNA.
Dantas
F J S
FJ
Departamento de Biofísica e Biometria, IBRAG, UERJ, Rio de Janeiro, RJ, Brazil.
de Mattos
J C P
JC
Moraes
M O
MO
Viana
M E
ME
Lage
C A S
CA
Cabral-Neto
J B
JB
Leitão
A C
AC
Bernardo-Filho
M
M
Bezerraa
R J A C
RJ
Carvalho
J J
JJ
Caldeira-de-Araújo
A
A
D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D009153:Mutagens / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000494:pharmacology; D017971:Tin Compounds / Q000633:toxicity*; D014343:Trypan Blue
D004396:Coloring Agents; D009153:Mutagens; D017382:Reactive Oxygen Species; D017971:Tin Compounds; C023599:stannous chloride; D014343:Trypan Blue