New directions for fluorescent speckle microscopy.
Fluorescent Speckle Microscopy (FSM) is a technology for analyzing cytoskeleton dynamics, giving novel insight into their roles in living cells. New applications of FSM, together with the development of computer-based FSM image analysis, will make FSM the first microscopy-based method to deliver quantitative kinetic readouts at high spatial and temporal resolution for a wide variety of macromolecular systems. Here, we review the most recent applications and developments and give a glimpse of future directions and potentials of FSM.
Waterman-Storer
Clare M
CM
Department of Cell Biology and Institute for Childhood and Neglected Diseases, The Scripps Research Institute, La Jolla, CA 92037, USA. waterman@scripps.edu
Danuser
Gaudenz
G
D000199:Actins / Q000378:metabolism; D000818:Animals; D001704:Biopolymers / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D005456:Fluorescent Dyes; D006801:Humans; D046911:Macromolecular Substances; D008856:Microscopy, Fluorescence / Q000379:methods*; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D009474:Neurons / Q000378:metabolism
D000199:Actins; D001704:Biopolymers; D005456:Fluorescent Dyes; D046911:Macromolecular Substances