title: Effect of psoralens on Fenton-like reaction generating reactive oxygen species.
abstract: Psoralens (psoralen, 5-methoxypsoralen, 8-methoxypsoralen, khellin, and visnagin) in 1 mM doses were shown to enhance the generation of reactive oxygen species, such as the hydroxyl radical (HO*), the superoxide anion radical (O2(-)), and singlet oxygen ((1)O(2)), from the system generating chemiluminescence (CL), as well as free radicals in the absence of light. The system that generated CL was made up of CoCl(2) and H(2)O(2). Incubation of psoralens in 0.2 mM doses with the generating system showed that only 8-methoxypsoralen and khellin have antioxidative effects. Antioxidative effects were also observed in the case of visnagin but in low concentration (0.05 mM). High doses of psoralens (1 mM) showed prooxidative effects. Measurements were done using a deoxyribose assay, the CL method, and spin-trapping with 5,5-dimethyl-1-pyrroline-N-oxide and 2,2,6,6-tetramethylpiperidine combined with electron spin resonance spectroscopy and spectrophotometry methods.
authors.affiliation: Pharmaceutical Analysis Laboratory, Biological & Medical Research Department (MBC-03-65), King Faisal Specialist Hospital & Research Centre, P.O. Box 3354, Riyadh 11211, Saudi Arabia. enein@kfshrc.edu.sa
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kladna
authors.forename: Irena
authors.initials: I
authors.lastname: Kruk
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Lichszteld
authors.forename: Teresa
authors.initials: T
authors.lastname: Michalska
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000138:chemical synthesis / Q000737:chemistry; D011564:Furocoumarins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D007700:Kinetics; D008163:Luminescent Measurements; D015394:Molecular Structure; D009589:Nitrogen Oxides / Q000138:chemical synthesis / Q000737:chemistry; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000138:chemical synthesis* / Q000737:chemistry; D013997:Time Factors
chemical_list: C045076:Fenton's reagent; D005609:Free Radicals; D011564:Furocoumarins; D009589:Nitrogen Oxides; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D007501:Iron; C039900:nitroxyl