title: Absence of voltage-dependent calcium channels delays photoreceptor degeneration in rd mice.
abstract: Retinal degeneration results from the apoptotic cell death of photoreceptors. While mutations in a large number of genes give rise to retinal degeneration, the specific mechanisms are not well understood. One hypothesis involves mediation of apoptosis by high concentrations of intracellular Ca(2+). We used a mouse line that carries the rd mutation but also lacks the major L-type voltage-dependent Ca(2+) channel at the photoreceptor synapse to examine whether this route of Ca(2+) entry plays a role in photoreceptor degeneration. In both experimental and control mice, the photoreceptors degenerate. However, at postnatal days 16, 18, and 21 there is a delay in photoreceptor cell loss in the experimental mice, which lack L-type voltage-dependent Ca(2+) channels, compared to controls. These data indicate that Ca(2+) entry via the L-type voltage-dependent Ca(2+) channel contributes to the mechanisms responsible for photoreceptor cell death in this mouse model of retinitis pigmentosa.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Louisville, KY 40202, USA.
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Read
authors.forename: Maureen A
authors.initials: MA
authors.lastname: McCall
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Gregg
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015220:Calcium Channels / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology*; D012160:Retina / Q000473:pathology / Q000503:physiopathology; D012162:Retinal Degeneration / Q000473:pathology / Q000503:physiopathology*; D012174:Retinitis Pigmentosa / Q000473:pathology / Q000503:physiopathology
chemical_list: D015220:Calcium Channels