title: The controversial nuclear matrix: a balanced point of view.
abstract: The nuclear matrix is defined as the residual framework after the removal of the nuclear envelope, chromatin, and soluble components by sequential extractions. According to several investigators the nuclear matrix provides the structural basis for intranuclear order. However, the existence itself and the nature of this structure is still uncertain. Although the techniques used for the visualization of the nuclear matrix have improved over the years, it is still unclear to what extent the isolated nuclear matrix corresponds to an in vivo existing structure. Therefore, considerable skepticism continues to surround the nuclear matrix fraction as an accurate representation of the situation in living cells. Here, we summarize the experimental evidence in favor of, or against, the presence of a diffuse nucleoskeleton as a facilitating organizational nonchromatin structure of the nucleus.
authors.affiliation: Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell'Apparato Locomotore, Sezione di Anatomia, Università di Bologna, Bologna, Italy. amartell@biocfarm.unibo.it
authors.forename: A M
authors.initials: AM
authors.lastname: Martelli
authors.forename: E
authors.initials: E
authors.lastname: Falcieri
authors.forename: M
authors.initials: M
authors.lastname: Zweyer
authors.forename: R
authors.initials: R
authors.lastname: Bortul
authors.forename: G
authors.initials: G
authors.lastname: Tabellini
authors.forename: A
authors.initials: A
authors.lastname: Cappellini
authors.forename: L
authors.initials: L
authors.lastname: Cocco
authors.forename: L
authors.initials: L
authors.lastname: Manzoli
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D015530:Nuclear Matrix / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism; D016707:Tissue Fixation
chemical_list: D009687:Nuclear Proteins