title: Combined use of the fully automated Bactec MGIT 960 system and a PCR-restriction fragment length polymorphism analysis for routine detection and identification of mycobacteria from clinical samples.
abstract: The combined use of Bactec MGIT 960 system and a PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis was assessed for the rapid detection and identification of mycobacteria from clinical samples. The diagnostic sensitivity and the time of detection of MGIT 960 system were significantly higher than of Löwenstein-Jensen medium. PCR-RFLP identification analysis results were in concordance with those obtained by the conventional biochemical tests.
authors.affiliation: Department of Microbiology, Medical School, University Hospital of Larissa, Papakyriazi 22, Larissa, Greece.
authors.forename: Fanourios
authors.initials: F
authors.lastname: Kontos
authors.forename: Efthymia
authors.initials: E
authors.lastname: Petinaki
authors.forename: Zoe
authors.initials: Z
authors.lastname: Gitti
authors.forename: Christos
authors.initials: C
authors.lastname: Costopoulos
authors.forename: Spyridoula
authors.initials: S
authors.lastname: Anagnostou
authors.forename: Ioannis
authors.initials: I
authors.lastname: Tselentis
authors.forename: Antonios N
authors.initials: AN
authors.lastname: Maniatis
mesh_terms: D001431:Bacteriological Techniques / Q000295:instrumentation*; D006801:Humans; D009164:Mycobacterium Infections / Q000175:diagnosis / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic