Comparative proteome analysis of human temporal cortex lobes by two-dimensional electrophoresis and identification of selected common proteins.
Human brain proteins were isolated from left and right temporal cortex lobes at the age of 73, 23, 84 years and separated by two-dimensional gel electrophoresis (2-DE). 2-DE was carried out with an immobilized pH gradient strip in the first dimension and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. Over 800 polypeptide spots were resolved with a silver-staining protocol by computerized 2-D gel analsis. Seven of the polypeptide spots were evidently distinguishable between human left and right temporal lobes. Four of the polypeptide spots were larger and three were smaller in human right temporal lobe. One of these three protein spots that have descendent expression in human right temporal lobe was identified as carbonyl reductase (NADPH) 1 by MALDI-TOF MS. Thirty-three common spots were identified by ESI-MS/MALDI-TOF MS/Edman sequencing and a protein database search. These identified proteins include some important enzymes and regulating proteins.
Chen
Wen
W
National Key Laboratory of Protein Engineering, College of Life Sciences, Peking University, Beijing, People's Republic of China.
Ji
Jianguo
J
Zhao
Rui
R
Ru
Binggen
B
D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000302:isolation & purification*; D010449:Peptide Mapping; D020543:Proteome; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013702:Temporal Lobe / Q000737:chemistry*
D009419:Nerve Tissue Proteins; D020543:Proteome