[Isolation, expression analysis of a chilling induced cDNA from rice root with differential display: an evidence role for caffeine-sensitive calcium signal].
Chilling-sensitive rice varieties acquire chilling tolerance when their roots are exposed to water stress for short time. Caffeine-sensitive calcium signal was involved in this procedure. By using total RNA differential display, a chilling induced cDNA(ICT: induction of chilling treatment) was isolated from roots of chilling-sensitive rice variety. It was determined that it is a novel cDNA by homology searching. The transcript level of ict mRNA is up-regulated under chilling stress, it is decreased to low level when the samples were transferred to standard culture conditions. Pre-treated with mannitol for two hours is beneficial to inducing ICT level of expression. This chilling induction was inhibited by caffeine, suggesting that it may play a putative role in signal transduction of caffeine-sensitive calcium.
Yin
Kui-De
KD
Heilongjiang August First Reclamation Agriculture University, Mishan 158308, China. Yinkuide@sohu.com
Zhang
Xing-Mei
XM
Liu
Shi-Qiang
SQ
Li
Le-Gong
LG
D003080:Cold Temperature; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008353:Mannitol / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects / Q000235:genetics*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; D012333:RNA, Messenger; D008353:Mannitol