title: New HLA-B alleles identified and sequences extended in potential bone marrow donors: B*5804, B*4418, B*1558, and B*4805.
abstract: Nucleic acid-based methods for allele identification have revealed more than 470 polymorphic variants at the HLA-B locus. Screening of potential bone marrow donors with sequence specific primer polymerase chain reactions and sequence specific oligonucleotide probe hybridization assays revealed apparent variants within the B*58, *44, *15, and *48 allele groups. DNA sequencing of cloned DNA identified the new alleles B*5804, B*4418, and B*1558 within these groups and observed new sequence information for the previously reported allele B*4805. These findings further extend our knowledge of the substantial genetic variation present at the HLA-B locus within human populations.
authors.affiliation: Department of Pathology, University of New Mexico, Albuquerque 87131-5051, USA. twilliams@salud.unm.edu
authors.forename: T M
authors.initials: TM
authors.lastname: Williams
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: S
authors.initials: S
authors.lastname: Bassinger
authors.forename: D
authors.initials: D
authors.lastname: Feldman
authors.forename: S
authors.initials: S
authors.lastname: Birkos
authors.forename: J
authors.initials: J
authors.lastname: Kearns
authors.forename: M
authors.initials: M
authors.lastname: Kamoun
mesh_terms: D000483:Alleles; D019943:Amino Acid Substitution / Q000235:genetics; D001483:Base Sequence; D044383:Black People / Q000235:genetics; D016026:Bone Marrow Transplantation; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D033581:Stem Cell Transplantation; D014019:Tissue Donors
chemical_list: D015235:HLA-B Antigens