Frequent polymorphic changes but rare tumor specific mutations of the LATS2 gene on 13q11-12 in esophageal squamous cell carcinoma.
We previously reported that loss of heterozygosity on 13q12-13 is related with poor prognosis of esophageal cancer. However, a target tumor-suppressor gene on this region is not yet identified. Recently, LATS2, a new human homologue of the Drosophila tumor suppressor gene (lats/warts) was identified on 13q11-12. We therefore screened esophageal tumor cell lines and tumor tissues to detect tumor specific mutations of the LATS2 gene. Although we found 5 different polymorphisms in this gene (4 kinds of single-base changes and a 6-bp insertion), a tumor specific mutation was identified in only one out of 60 tumor tissues. These results indicated that the LATS2 gene is inactivated in only a small part of esophageal tumors, if any.
Ishizaki
Kanji
K
Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan. kishizaki@aichi-cc.jp kishizaki@aichi-cc.jp
Fujimoto
Jiro
J
Kumimoto
Hiroshi
H
Nishimoto
Yoshio
Y
Shimada
Yutaka
Y
Shinoda
Masayuki
M
Yamamoto
Tadashi
T
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002882:Chromosomes, Human, Pair 13; D004273:DNA, Neoplasm / Q000032:analysis; D004938:Esophageal Neoplasms / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D009154:Mutation; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
D004273:DNA, Neoplasm; D025521:Tumor Suppressor Proteins; D011494:Protein Kinases; C406449:LATS2 protein, human; D017346:Protein Serine-Threonine Kinases