[Expression of precore and core proteins of hepatitis B virus in human hepatoma cell line].
To further investigate the functions of proteins encoded by C open reading frame of hepatitis B virus (HBV) in HBV infectious cycle, cell lines expressing these proteins were established. The DNA fragments encoding 25 000, 22 000 precore-core and 21 000 core protein respectively were amplified from plasmid pCP10 which contained HBV genome, and inserted into a eukaryotic expression vector pcDNA-3, and then transfected into hepatoma cell line HepG2. The transformants were selected with neomycin, and three isolated colonies expressing precore or core proteins were obtained stably, which were demonstrated by immunohistochemistry and Western blotting analysis.
Liu
Ding-Xie
DX
Department of Infectious Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Dai
Lin
L
Luo
Kang-Xian
KX
D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006515:Hepatitis B virus / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D014162:Transfection; D014407:Tumor Cells, Cultured; D014758:Viral Core Proteins / Q000096:biosynthesis* / Q000235:genetics
D014758:Viral Core Proteins