Exogenous protein kinases A and C, but not endogenous prostasome-associated protein kinase, phosphorylate semenogelins I and II from human semen.
Semenogelins I and II are the quantitatively dominating proteins in human semen. They comprise the major part of the sperm-entrapping gel formed at ejaculation, which subsequently liquefies due to proteolysis of the gel-forming proteins by prostate-specific antigen (PSA). The mechanism behind gel formation and its physiological significance is not known. We have studied phosphorylation and dephosphorylation of human semenogelins. Both were phosphorylated by protein kinases A and C (PKA and PKC, respectively) at a rate about 5 times less than that of histone. For PKA, incorporated ((32)P)phosphate into semenogelin approached a maximum above 1 mol/mol. Corresponding values for phosphorylation of the semenogelins with PKC were greater than 10. There was no change in the sensitivity of phosphosemenogelins to proteolysis by PSA. Serine (PKA) and serine and threonine (PKC) were the phosphate-accepting amino acid residues, and all incorporated ((32)P)phosphate could be removed from the semenogelins with human acid phosphatase. Nil or very little phosphate could be detected in purified semenogelins isolated from seminal plasma. In vivo, about half the protein kinase activity in seminal plasma was bound to prostasomes. PKA but not PKC purified from prostasomes could phosphorylate specific substrates, but they could phosphorylate either of the semenogelins.
Ek
Pia
P
Department of Medical Biochemistry and Microbiology, University of Uppsala, Sweden. pia.ek@imbim.uu.se
Malm
Johan
J
Lilja
Hans
H
Carlsson
Lena
L
Ronquist
Gunnar
G
D000135:Acid Phosphatase / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000494:pharmacology*; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D006801:Humans; D008297:Male; D009097:Multienzyme Complexes / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D017430:Prostate-Specific Antigen / Q000494:pharmacology; D046988:Proteasome Endopeptidase Complex; D011493:Protein Kinase C / Q000494:pharmacology*; D011494:Protein Kinases / Q000502:physiology*; D029589:Seminal Plasma Proteins / Q000378:metabolism*; D029607:Seminal Vesicle Secretory Proteins / Q000378:metabolism*
D000596:Amino Acids; D009097:Multienzyme Complexes; D010710:Phosphates; D029589:Seminal Plasma Proteins; D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen; D011494:Protein Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D000135:Acid Phosphatase; D017430:Prostate-Specific Antigen; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex