title: Soluble interleukin-1 receptor type II blocks monocyte chemotactic protein-1 secretion by U937 cells in response to peripheral blood serum of women with endometriosis.
abstract: OBJECTIVE
  To assess the ability of peripheral blood serum from women with endometriosis to induce monocyte chemotactic protein-1 (MCP-1) secretion by monocytes and the putative role of the interleukin-1 (IL-1) system in endometriosis-associated monocyte activation.
  
  
  METHODS
  Cultures of U937 monocytic cells exposed to serum from normal women (control group) or women with endometriosis.
  
  
  METHODS
  Gynecology clinic and human reproduction research laboratory.
  
  
  METHODS
  Seventy-nine women with endometriosis and 38 control women with no evidence of endometriosis at laparoscopy.
  
  
  METHODS
  Peripheral blood obtained a few days before laparoscopy.
  
  
  METHODS
  MCP-1 secretion in the culture medium and serum concentrations of soluble IL-1 receptor type II (sIL-1RII), IL-1beta, and IL-1alpha by ELISA or by enzyme immunometric assay.
  
  
  RESULTS
  Serum concentrations of sIL-1RII were significantly lower in women with stage I-II endometriosis than in control women, whereas serum concentrations of IL-1beta and IL-1alpha were comparable between the two groups. The serum of women with endometriosis induced higher secretion of MCP-1 by U937 cells than that of control women, particularly in the initial stages of endometriosis (stages I-II), and recombinant IL-1RII (rIL-1RII) significantly blocked that secretion.
  
  
  CONCLUSIONS
  These findings point toward a deficiency in the mechanisms involved in the down-regulation of IL-1 actions at the systemic level and reveal sIL-1RII as a key factor involved in that process.
authors.affiliation: Département d'Obstétrique-Gynécologie, Faculté de Médecine, Université Laval, Québec, Québec, Canada.
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Kharfi
authors.forename: Ali
authors.initials: A
authors.lastname: Akoum
mesh_terms: D018932:Chemokine CCL2 / Q000378:metabolism*; D003470:Culture Media; D004715:Endometriosis / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D009000:Monocytes / Q000502:physiology*; D017472:Receptors, Interleukin-1 / Q000097:blood / Q000235:genetics / Q000502:physiology*; D053574:Receptors, Interleukin-1 Type II; D011994:Recombinant Proteins / Q000494:pharmacology; D012995:Solubility; D020298:U937 Cells
chemical_list: D018932:Chemokine CCL2; D003470:Culture Media; D017472:Receptors, Interleukin-1; D053574:Receptors, Interleukin-1 Type II; D011994:Recombinant Proteins