Expression and function of phospholipase A(2) in brain.
Phospholipase A(2) (PLA(2)) appears to play a fundamental role in cell injury in the central nervous system. We have investigated PLA(2) expression in the astrocytoma cell line 1231N1, and found that GIVA, GIVB, GIVC and GVI PLA(2) messages are expressed. PLA(2) activity is increased by inflammatory/injury stimuli such as interleukin-1beta and lipopolysaccharide in these cells but with very different time courses. The arachidonic acid liberated is converted to prostaglandin E(2), possibly by cyclooxygenase-2, which is induced by inflammatory stimuli. This cell system emerges as a model to study injury/inflammation-related activation of the new PLA(2) forms GIVB and GIVC.
Balboa
María A
MA
Department of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0601, USA.
Varela-Nieto
Isabel
I
Killermann Lucas
Karin
K
Dennis
Edward A
EA
D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D001254:Astrocytoma / Q000378:metabolism; D001921:Brain / Q000201:enzymology*; D001932:Brain Neoplasms / Q000378:metabolism; D051546:Cyclooxygenase 2; D018076:DNA, Complementary / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007249:Inflammation; D007375:Interleukin-1 / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D063065:Organophosphonates / Q000494:pharmacology; D010741:Phospholipases A / Q000096:biosynthesis* / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D014407:Tumor Cells, Cultured
D001095:Arachidonic Acids; D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D007375:Interleukin-1; D007527:Isoenzymes; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D063065:Organophosphonates; C104810:methyl arachidonylfluorophosphonate; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A; D015232:Dinoprostone