Activity of adenosine deaminase and its isoforms in pleural fluid in tuberculous pleuritis.
BACKGROUND
The problem of tuberculosis is increasing in a number of countries. Adenosine deaminase activity is considered in many clinics to be a valuable biochemical test of this pathology. Considerable research has been devoted to the activity of enzyme isoforms as significant tests for diagnosing tuberculosis. The aim of our study was to compare the significance of different adenosine deaminase dependent parameters in diagnosing tuberculosis.


METHODS
The level of adenosine deaminase and the activity of its two isoenzymes in the pleural fluids of patients with tuberculous and non-tuberculous pleurisy were compared.


RESULTS
The adenosine deaminase level in tuberculous pleural effusions was higher than in non-tuberculous pleural effusions. The data we obtained suggest that the enzyme activity level could be a very reliable test in the differential diagnosis of tuberculous pleurisy in the Armenian population. The activity of isoenzymes ADA1 and ADA2, or their ratios to the total ADA activity, though valuable information, has no diagnostic advantage over total ADA activity in diagnosing this pathology.


CONCLUSIONS
The results clearly point up the value of using a total ADA activity assay in Armenian clinics for the differential diagnosis of tuberculous pleurisy. Determinations of the activity level of the ADA1 and ADA2 isoenzymes provide no diagnostic advantage over total ADA activity.
Andreasyan
Nune A
NA
H. Buniatian Institute of Biochemistry, National Academy of Sciences, Republic of Armenia.
Hairapetian
Hripsime L
HL
Sargisova
Yelizaveta G
YG
Mardanyan
Sona S
SS
Badalyan
Lena T
LT
Khanoyan
Armen S
AS
D000243:Adenosine Deaminase / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D001826:Body Fluids / Q000201:enzymology*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010996:Pleural Effusion; D012189:Retrospective Studies; D013223:Statistics as Topic; D014396:Tuberculosis, Pleural / Q000175:diagnosis / Q000201:enzymology*
D007527:Isoenzymes; D000243:Adenosine Deaminase