title: The promoter polymorphism of the matrix metalloproteinase 3 (MMP-3) gene in women with ovarian cancer.
abstract: Matrix metalloproteinases (MMPs) comprise family proteolytic enzymes that degrade extracellular matrix components and therefore play an important role in tumour cell invasion and cancer metastasis. Overexpression of matrix metalloproteinase 3 (MMP-3 or stromelysin 1) gene has been demonstrated in various types of cancers and high level of MMP-3 protein in tumour is a poor prognostic factor for patients. The insertion (6A)/deletion (5A) polymorphism (5A/6A polymorphism) located at the promoter of the MMP-3 gene may have functional significance in the regulation of its expression. In the present work the distribution of genotypes and frequencies of alleles of the 5A/6A polymorphism in subjects with ovarian cancer were investigated. Paraffin embedded tumour tissues were obtained from 100 women with ovarian cancer. The genotypes of 5A/6A polymorphism were determined by PCR amplification using the allele specific primers. The distribution of the genotypes of the 5A/6A polymorphism in both control and patients did not differ significantly (p > 0.05) from those predicted by the Hardy-Weinberg distribution. Additionally, there were no significant differences (p > 0.05) in genotype distributions and allele frequencies between subgroups assigned to histological stage. The results suggest that the 5A/6A polymorphism of MMP-3 gene may not be linked with appearance and development to ovarian cancer.
authors.affiliation: Dept. of Surgical Gynaecology, Institute of Polish Mother's Memorial Hospital, Lodz.
authors.forename: K
authors.initials: K
authors.lastname: Szyllo
authors.forename: B
authors.initials: B
authors.lastname: Smolarz
authors.forename: H
authors.initials: H
authors.lastname: Romanowicz-Makowska
authors.forename: M
authors.initials: M
authors.lastname: Niewiadomski
authors.forename: E
authors.initials: E
authors.lastname: Kozlowska
authors.forename: A
authors.initials: A
authors.lastname: Kulig
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005838:Genotype; D006801:Humans; D019656:Loss of Heterozygosity; D019278:Matrix Metalloproteinase 3 / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic
chemical_list: D017931:DNA Primers; D019278:Matrix Metalloproteinase 3