title: A new improved method for the concentration of HIV-1 infective particles.
abstract: Improvement of the sensitivity of detection systems for human immunodeficiency virus-1 (HIV-1) has been carried out. One approach to improve the sensitivity is purification and/or concentration of the virus from a specimen. In this study, a method for concentrating HIV-1 using polyethylene glycol (PEG) has been re-evaluated and the optimal protocol for concentrating the virus from low-titer specimens was determined. That is, to obtain a virus pellet, a mixture of equal volumes of a specimen and 20% PEG 20,000 solution in saline is incubated at 4 degrees C for 16 h and then centrifuged at 17860 x g in a microcentrifuge for 20 min. HIV-1 in the pellet could be detectable by HIV-1 p24 antigen capture assay for viral protein, reverse transcriptase (RT) assay for viral enzyme, reverse transcriptase polymerase chain reaction (RT-PCR) assay for viral RNA and a virus infectivity assay.
authors.affiliation: Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Kohno
authors.forename: Sharad
authors.initials: S
authors.lastname: Mohan
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Goto
authors.forename: Chizuko
authors.initials: C
authors.lastname: Morita
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakano
authors.forename: Wu
authors.initials: W
authors.lastname: Hong
authors.forename: Joseph Carlo E
authors.initials: JC
authors.lastname: Sangco
authors.forename: Shinichi
authors.initials: S
authors.lastname: Morimatsu
authors.forename: Kouichi
authors.initials: K
authors.lastname: Sano
mesh_terms: D002460:Cell Line; D002498:Centrifugation; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008828:Microbiological Techniques; D011092:Polyethylene Glycols; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011092:Polyethylene Glycols