DNA microarrays: implications for clinical medicine.
DNA microarrays are capable of measuring the expression of thousands of genes in a single assay. This technology has led to an explosion in global gene expression profiling studies, allowing researchers to more fully explore a multitude of complex biological systems ranging from the yeast cell cycle to the progression of cancer. By analyzing collections of these global profiles, the molecular underpinnings of biological phenomena are being unraveled. It is the goal of this review to address the basic principles of the technology and methodology employed in gene expression profiling with DNA microarrays, and then to highlight recent studies with particular importance for clinical medicine, focusing on cancer.
Rhodes
Daniel R
DR
Department of Pathology, Urology, and the Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor 48109, USA.
Chinnaiyan
Arul M
AM
D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*