Penicillium digitatum metabolites on synthetic media and citrus fruits.
Penicillium digitatum has been cultured on citrus fruits and yeast extract sucrose agar media (YES). Cultivation of fungal cultures on solid medium allowed the isolation of two novel tryptoquivaline-like metabolites, tryptoquialanine A (1) and tryptoquialanine B (2), also biosynthesized on citrus fruits. Their structural elucidation is described on the basis of their spectroscopic data, including those from 2D NMR experiments. The analysis of the biomass sterols led to the identification of 8-12. Fungal infection on the natural substrates induced the release of citrus monoterpenes together with fungal volatiles. The host-pathogen interaction in nature and the possible biological role of citrus volatiles are also discussed.
Ariza
Marta R
MR
Department of Organic Chemistry, University of Granada, Avdn. Fuentenueva 18071, Spain.
Larsen
Thomas O
TO
Peterson
Bent O
BO
Duus
Jens O
JO
Barrero
Alejandro F
AF
D002957:Citrus / Q000382:microbiology*; D003470:Culture Media; D005516:Food Microbiology; D007211:Indoles / Q000032:analysis / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009183:Mycotoxins / Q000032:analysis / Q000378:metabolism; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D007211:Indoles; D009183:Mycotoxins; C010522:tryptoquivaline