The use of DODT as a non-malodorous scavenger in Fmoc-based peptide synthesis.
We have synthesized a random group of peptides and performed cleavages using various cleavage cocktails including 3,6-dioxa-1,8-octanedithiol (DODT). Purity of the peptides was compared to that obtained with standard protocols for cleavage using RP-HPLC and Maldi-Tof mass spectrometry. We show that stinking thiols can be replaced by the almost odourless (DODT) without negatively affecting the purity of the end product.
Teixeira
A
A
Department of Immunohematology and Bloodtransfusion, Building 1, E3-Q, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
Benckhuijsen
W E
WE
de Koning
P E
PE
Valentijn
A R P M
AR
Drijfhout
J W
JW
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D005019:Ethyl Ethers / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009812:Odorants; D010455:Peptides / Q000138:chemical synthesis*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
C471080:3,6-dioxa-1,8-octanedithiol; D005019:Ethyl Ethers; D010455:Peptides; D013438:Sulfhydryl Compounds