Melting curve analysis of feline calicivirus isolates detected by real-time reverse transcription PCR.
A real-time reverse transcription polymerase chain reaction assay was developed to detect feline calicivirus infection in cats. Using SYBR green I melting curve analysis, isolates could be distinguished by their melting temperature. The assay is sensitive, linear over a wide range of template concentrations and allows accurate quantitation of viral load.
Helps
Chris
C
Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol BS40 5DU, UK. c.r.helps@bristol.ac.uk
Lait
Philippa
P
Tasker
Séverine
S
Harbour
Dave
D
D000818:Animals; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017927:Calicivirus, Feline / Q000235:genetics / Q000302:isolation & purification*; D002371:Cat Diseases / Q000175:diagnosis* / Q000821:virology; D002415:Cats; D009691:Nucleic Acid Denaturation; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary