title: Enteropathogens associated with acute diarrhea in community and hospital patients in Jakarta, Indonesia.
abstract: The prevalence of bacteria, parasite and viral pathogens in 3875 patients with diarrhea in community and hospital settings from March 1997 through August 1999 in Jakarta, Indonesia was determined using routine bacteriology and molecular assay techniques. Bacterial pathogens isolated from hospital patients were, in decreasing frequency, Vibrio cholerae O1, Shigella flexneri, Salmonella spp. and Campylobacter jejuni, while S. flexneri, V. cholerae O1, Salmonella spp. and C. jejuni were isolated from the community patients. V. cholerae O1 was isolated more frequently (P<0.005) from the hospital patients than the community patients. Overall, bacterial pathogens were isolated from 538 of 3875 (14%) enrolled cases of diarrhea. Enterotoxigenic Escherichia coli were detected in 218 (18%) of 1244 rectal swabs. A small percentage of enterohemorrhagic E. coli (1%) and of Clostridium difficile (1.3%) was detected. Parasitic examination of 389 samples resulted in 43 (11%) positives comprising Ascaris lumbricoides (1.5%), Blastocystis hominis (5.7%), Giardia lamblia (0.8%), Trichuris trichiura (2.1%) and Endolimax nana (0.5%). Rotavirus (37.5%), adenovirus (3.3%) and Norwalk-like virus (17.6%) were also detected. Antimicrobial resistance was observed among some isolates. Bacterial isolates were susceptible to quinolones, with the exception of some isolates of C. jejuni which were resistant to ciprofloxacin, nalidixic acid and norfloxacin. Data obtained from this community- and hospital-based study will enable the Indonesian Ministry of Health to plan relevant studies on diarrheal diseases in the archipelago.
authors.affiliation: US Naval Medical Research Unit No 2, Jakarta, Indonesia. oyofoba@namru2.med.navy.mil
authors.forename: Buhari A
authors.initials: BA
authors.lastname: Oyofo
authors.forename: Decy
authors.initials: D
authors.lastname: Subekti
authors.forename: Periska
authors.initials: P
authors.lastname: Tjaniadi
authors.forename: Nunung
authors.initials: N
authors.lastname: Machpud
authors.forename: S
authors.initials: S
authors.lastname: Komalarini
authors.forename: B
authors.initials: B
authors.lastname: Setiawan
authors.forename: C
authors.initials: C
authors.lastname: Simanjuntak
authors.forename: Narain
authors.initials: N
authors.lastname: Punjabi
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Corwin
authors.forename: Momtaz
authors.initials: M
authors.lastname: Wasfy
authors.forename: James R
authors.initials: JR
authors.lastname: Campbell
authors.forename: Murad
authors.initials: M
authors.lastname: Lesmana
mesh_terms: D000208:Acute Disease; D000328:Adult; D000818:Animals; D002648:Child; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology* / Q000469:parasitology; D004064:Digestive System / Q000382:microbiology / Q000469:parasitology / Q000473:pathology; D024881:Drug Resistance, Bacterial; D019353:Endemic Diseases / Q000145:classification / Q000517:prevention & control / Q000706:statistics & numerical data; D006760:Hospitalization; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D015995:Prevalence; D011446:Prospective Studies; D012111:Residence Characteristics