title: Expression of fibroblast growth factors in rat dorsal root ganglion neurons and regulation after peripheral nerve injury.
abstract: Using cDNA array, we observed the expression of eight members of the fibroblast growth factor (FGF) family, FGF 2, 5, 7, 9, 10, 13 and 14, in rat lumbar 4 and 5 dorsal root ganglia (DRGs). Over a period of 28 days after sciatic nerve transection, the array signals for FGF 2 and 7 were significantly increased in the DRGs, while FGF 13 decreased. Using the reverse transcription polymerase chain reaction (RT-PCR), we confirmed the axotomy-induced changes in the expression of FGF 7 and 13. hybridization showed that FGF 13 was expressed in 60% of DRG neurons under normal circumstance. Seven days after axotomy the number of FGF 13-positive neurons was decreased to 18%, but partially recovered to 40% after 28 days. FGF 13 immunoreactivity was also decreased. These data indicate that FGFs are important for DRG neurons under normal circumstance and after nerve injury.
authors.affiliation: Laboratory of Sensory System, Institute of Neuroscience, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, 200031, China.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Wo
authors.forename: Mei-Fang
authors.initials: MF
authors.lastname: Zhong
authors.forename: Fang-Xiong
authors.initials: FX
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Bao
authors.forename: Ying-Jin
authors.initials: YJ
authors.lastname: Lu
authors.forename: Yao-De
authors.initials: YD
authors.lastname: Huang
authors.forename: Hua-Sheng
authors.initials: HS
authors.lastname: Xiao
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000235:genetics; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000378:metabolism; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000503:physiopathology; D015870:Gene Expression / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D009437:Neuralgia / Q000378:metabolism* / Q000503:physiopathology; D009475:Neurons, Afferent / Q000378:metabolism*; D059348:Peripheral Nerve Injuries; D010523:Peripheral Nervous System Diseases / Q000378:metabolism* / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000502:physiology; D012584:Sciatic Nerve / Q000293:injuries / Q000503:physiopathology / Q000601:surgery; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D005346:Fibroblast Growth Factors