title: Induction of apoptosis by penta-acetyl geniposide in rat C6 glioma cells.
abstract: Penta-acetyl geniposide, (Ac)(5)-GP, was produced by acetylation of a glycoside, isolated from an extract of Gardenia fructus. Previously, we have reported that C6 glioma cells could be inhibited in culturing as well as in bearing rats by treating with (Ac)(5)-GP. In this study, the effect and action of (Ac)(5)-GP on inducing cell death was examined in rat C6 glioma cells. Treatment of C6 glioma cells with (Ac)(5)-GP caused cell death, chromatin condensation, and internucleosomal DNA ladder. Also, cell cycle arrest at G(0)/G(1) phase revealed that (Ac)(5)-GP-induced cell death appears to be mediated by apoptosis. In addition, the results also showed that p53 and c-Myc increased due to treatment of (Ac)(5)-GP in a dose-response and time-dependent manner. Concomitant with the expression of p53 and c-Myc, decreased level of Bcl-2 and increased level of Bax protein were observed. These results suggest that cell death caused by (Ac)(5)-GP through apoptosis and cell cycle arrest at G(0)/G(1) may be associated with the induction of p53, c-Myc and may be mediated with apoptosis-related Bcl-2 family proteins.
authors.affiliation: Institute of Biochemistry, Chung Shan Medical University, No. 110, Section 2, Chien Kuo N. Road, Taichung, Taiwan.
authors.forename: Yun Ching
authors.initials: YC
authors.lastname: Chang
authors.forename: Tsui Hwa
authors.initials: TH
authors.lastname: Tseng
authors.forename: Miao Jane
authors.initials: MJ
authors.lastname: Lee
authors.forename: Jeng Dong
authors.initials: JD
authors.lastname: Hsu
authors.forename: Chau Jong
authors.initials: CJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D032062:Gardenia / Q000737:chemistry; D005910:Glioma / Q000473:pathology*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D011714:Pyrans / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051028:bcl-2-Associated X Protein
chemical_list: C469218:1-(2',3',4',6'-tetraacetylglucopyranosyloxyl)-1,4a,5,7a-tetrahydro-7-(acetomethyl)-cyclopentapyran-4-caroboxylic acid methyl ester; C494828:Bax protein, rat; D010936:Plant Extracts; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016271:Proto-Oncogene Proteins c-myc; D011714:Pyrans; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein