Identification of atypical strains of Malassezia spp. from cattle and dog.
Yeast species in the genus Malassezia are lipophilic with the exception of Malassezia pachydermatis. During a study of the occurrence of Malassezia species in the external ear of 964 cattle and 6 dogs in Minas Gerais, Brazil, six lipid-dependent isolates could not be identified to known species. Four isolates came from healthy cows, one from a cow with otitis, and one from a healthy dog. When tested with Tweens and Cremophor EL as single sources of lipids, the strains grew on all sources except Cremophor EL. None of the six strains hydrolyzed esculin, and all produced catalase. Pigment production from tryptophan was variable. Partial large subunit rRNA sequences were obtained for two isolates that remained viable in culture. The strain from the cow with otitis was identified as a lipid-dependent variant of M. pachydermatis, and the strain from the dog was an atypical variant of Malassezia furfur.
Duarte
Eduardo Robson
ER
Department of Microbiology, Biological Sciences Institute, Federal University of Minas Gerais, Brazil.
Lachance
Marc-André
MA
Hamdan
Júnia Soares
JS
D000818:Animals; D001483:Base Sequence; D001717:Birds; D002417:Cattle / Q000382:microbiology*; D003470:Culture Media; D004275:DNA, Ribosomal / Q000032:analysis; D004285:Dogs / Q000382:microbiology*; D006801:Humans; D008290:Malassezia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data
D003470:Culture Media; D004275:DNA, Ribosomal