[Purification of PCR products with cetyltrimethylammonium bromide].
OBJECTIVE
To establish a method for purifying PCR products with cetyltrimethylammonium bromide (CTAB).


METHODS
Selective precipitation of the PCR product was performed using CTAB, which forms compound with DNA fragment in salt solution of appropriate concentration, but not with single strain oligonucleotide or dNTPs. The precipitation could be dissolved in 1.2 mol/L NaCl while the addition of ethanol caused the desired PCR product to precipitate so as to be recovered.


RESULTS
The primers and small-molecule dNTP could be effectively eliminated after this procedure. Although the output of the purification process reached only 80% that by current reagent kit, it reduces the cost to as low as 1/8 of that normally required by the kit.


CONCLUSIONS
CTAB is applicable for purification of the PCR product.
Zhang
Bao
B
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China. zhangb@fimmu.edu.cn
Ma
Wen-Li
WL
Liu
Li-Yang
LY
Wu
Qing-Hua
QH
Guo
Qiu-Ye
QY
Zheng
Wen-Ling
WL
D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D011232:Chemical Precipitation; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods
D002593:Cetrimonium Compounds; D004247:DNA; D000077286:Cetrimonium