title: Identification of the first isolates of Trichosporon asahii var asahii from disseminated trichosporonosis in China.
abstract: Infection with Trichosporon asahii is a major cause of deep-seated and disseminated trichosporonosis, which is associated with a high mortality rate. Disseminated trichosporonosis in individuals with no underlying disease has not been reported. In this study, we report the identification of the first isolate of Trichosporon asahii var. asahii in China. Two isolates were obtained from the liver and skin of a patient with disseminated trichosporonosis who displayed no evidence of underlying disease. The morphologic and physiologic characteristics of the two isolates differed slightly from those of usual strains of T. asahii var. asahii, including the type strain CBS 2479. Ubiquinone-9 was identified as the major ubiquinone in both isolates. Sequence analysis of the LSUrDNA D1/D2, ITS, and IGS1 regions from the two isolates showed them to be T. asahii var. asahii, and random amplified polymorphic DNA analysis strongly suggested that they were the same strain.
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan. kfuky@myco.pf.chiba-u.ac.jp
authors.forename: Paride
authors.initials: P
authors.lastname: Abliz
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Fukushima
authors.forename: Kayoko
authors.initials: K
authors.lastname: Takizawa
authors.forename: Rongya
authors.initials: R
authors.lastname: Yang
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Li
authors.forename: Kazuko
authors.initials: K
authors.lastname: Nishimura
mesh_terms: D000328:Adult; D001483:Base Sequence; D002681:China; D015169:Colony Count, Microbial; D004271:DNA, Fungal / Q000032:analysis*; D005260:Female; D016469:Fungemia / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D009181:Mycoses / Q000175:diagnosis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014250:Trichosporon / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004271:DNA, Fungal