title: Role of MMPs and plasminogen activators in angiogenesis after transmyocardial laser revascularization in dogs.
abstract: We examined the role of matrix metalloproteinases (MMPs), tissue inhibitors of MMP (TIMPs), and plasminogen activator (PA) in transmyocardial laser revascularization (TMLR)-induced angiogenesis. TMLR was accomplished with a carbon dioxide laser in seven dogs whose left anterior descending coronary artery (LAD) was ligated. Seven control dogs underwent only LAD ligation, and four dogs underwent a sham operation, consisting only of a left thoracotomy. Two weeks later, transmural myocardial samples were harvested from the distributions of the LAD and the left circumflex artery for substrate zymography, immunohistochemical staining, and in situ zymography. MMP-1, MMP-2, TIMP-1, TIMP-2, and urokinase-type PA levels in the distribution of the LAD were higher in the laser group than in the control or sham group. Counts of von Willebrand factor-positive microvessels and smooth muscle alpha-actin-positive arterioles demonstrated that the angiogenesis and ateriogenesis was promoted in the laser group and correlated directly with the number of MMP-stained microvessels. We conclude that TMLR induces the expression of MMPs, TIMPs, and urokinase-type PA and that these proteinases play an important role in angiogenesis after TMLR.
authors.affiliation: Second Department of Surgery, Fukui Medical University, Japan.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Kuniyoshi
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yukio
authors.initials: Y
authors.lastname: Chiba
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kimura
authors.forename: Kouichi
authors.initials: K
authors.lastname: Morioka
authors.forename: Takahiko
authors.initials: T
authors.lastname: Uesaka
authors.forename: Akio
authors.initials: A
authors.lastname: Ihaya
authors.forename: Masato
authors.initials: M
authors.lastname: Sasaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsuda
authors.forename: Narihisa
authors.initials: N
authors.lastname: Yamada
mesh_terms: D000818:Animals; D004285:Dogs; D004699:Endocardium / Q000473:pathology; D018093:Gelatinases / Q000378:metabolism; D007150:Immunohistochemistry; D053685:Laser Therapy; D020782:Matrix Metalloproteinases / Q000502:physiology*; D009204:Myocardial Revascularization; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010496:Pericardium / Q000473:pathology; D010960:Plasminogen Activators / Q000502:physiology*; D011184:Postoperative Period; D012333:RNA, Messenger / Q000378:metabolism; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D010960:Plasminogen Activators; D018093:Gelatinases; D020782:Matrix Metalloproteinases