Biological variability in clinical isolates of Trichomonas vaginalis.
Eighteen clinical isolates of Trichomonas vaginalis were obtained from women who attended health centers of the Government of Madrid. A total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid Diamond medium. Pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. As could be expected, a broad variability was obtained, being some of the isolates more virulent than the reference strain. Regarding to metronidazole susceptibility, none resistant isolate was found but different degrees of susceptibility were determined.
Gómez-Barrio
Alicia
A
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, Madrid, España. agbarrio@farm.ucm.es
Nogal-Ruiz
Juan J
JJ
Montero-Pereira
David
D
Rodríguez-Gallego
Ester
E
Romero-Fernandez
Esperanza
E
Escario
José A
JA
D000818:Animals; D000994:Antitrichomonal Agents / Q000494:pharmacology; D005260:Female; D006801:Humans; D008795:Metronidazole / Q000494:pharmacology; D051379:Mice; D021261:Parasitic Sensitivity Tests; D014245:Trichomonas Infections / Q000188:drug therapy / Q000469:parasitology*; D014246:Trichomonas vaginalis / Q000187:drug effects / Q000472:pathogenicity*; D014774:Virulence
D000994:Antitrichomonal Agents; D008795:Metronidazole