title: Optimization of fermentation conditions for production of glycopeptide antibiotic vancomycin by Amycolatopsis orientalis.
abstract: Glycopeptides produced by Streptomyces species are the drugs used against beta-lactam drug-resistant staphylococcal infections, and vancomycin is important among them. Increased prevalence of resistant strains increased the usage of vancomycin worldwide and also promoted attempts for indigenous production. The optimum process conditions pH, temperature, inoculum size, agitation, and aeration for vancomycin production by Amycolatopsis orientalis were evaluated, statistically analyzed, and the response surface curves were constructed. The optimum process conditions were a pH of 7.6, a temperature of 29 degrees C, an inoculum size of 4.5%, an agitation of 255 rpm, and an aeration of less than 1:10 medium-to-air ratio.
authors.affiliation: Department of Microbiology, Osmania University, Hyderabad, India.
authors.forename: P Naga
authors.initials: PN
authors.lastname: Padma
authors.forename: A Bhaskar
authors.initials: AB
authors.lastname: Rao
authors.forename: J S
authors.initials: JS
authors.lastname: Yadav
authors.forename: Gopal
authors.initials: G
authors.lastname: Reddy
mesh_terms: D000192:Actinomycetales / Q000145:classification / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D019149:Bioreactors; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D015233:Models, Statistical; D010100:Oxygen / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014640:Vancomycin / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D014640:Vancomycin; D010100:Oxygen