Histone deacetylase 3 binds to and regulates the multifunctional transcription factor TFII-I.
Histone deacetylase 3 (HDAC3) is one of four members of the human class I histone deacetylases that are implicated in transcriptional repression through deacetylation of acetyllysines in amino-terminal tails of core histones. In an immunoaffinity purification using anti-HDAC3, transcription factor TFII-I copurified with HDAC3. Specificity of the HDAC3-TFII-I interaction was confirmed by coimmunoprecipitation of epitope-tagged proteins, GST pull-down assays, and protein colocalization with indirect immunofluorescence. An anti-TFII-I immunoprecipitate contained histone deacetylase enzymatic activity. Mutational analyses revealed that the carboxyl-terminal of HDAC3 (residues 373-401) and residues 363-606 of TFII-I were required for the HDAC3-TFII-I interaction. Transcriptional activation by TFII-I was severely reduced by overexpression of HDAC3. These results suggest that HDAC3 modulates some of the functions of TFII-I and provides a link between histone deacetylase and a multifunctional transcriptional activator.
Wen
Yu-Der
YD
H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida 33612, USA.
Cress
W Douglas
WD
Roy
Ananda L
AL
Seto
Edward
E
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D019084:Fluorescent Antibody Technique, Indirect; D006655:Histone Deacetylases / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D019473:Transcription Factors, TFII / Q000737:chemistry / Q000378:metabolism*; D000099186:Histone Deacetylase 3
D006655:Histone Deacetylases; D019473:Transcription Factors, TFII; D000099186:Histone Deacetylase 3; C104259:GTF2I protein, human