The use of field emission scanning electron microscopy to assess recombinant adenovirus stability.
A field emission scanning electron microscopy (FESEM) method was developed to assess the stability of a recombinant adenovirus (rAd). This method was designed to simultaneously sort, count, and size the total number of rAd viral species observed within an image field. To test the method, a preparation of p53 transgene-expressing recombinant adenovirus (rAd/p53) was incubated at 37 degrees C and the viral particles were evaluated by number, structure, and degree of aggregation as a function of time. Transmission electron microscopy (TEM) was also used to obtain ultrastructural detail. In addition, the infectious activity of the incubated rAd/p53 samples was determined using flow cytometry. FESEM image-analysis revealed that incubation at 37 degrees C resulted in a time-dependent decrease in the total number of detectable single rAd/p53 virus particles and an increase in apparent aggregates composed of more than three adenovirus particles. There was also an observed decrease in both the diameter and perimeter of the single rAd/p53 viral particles. TEM further revealed the accumulation of damaged single particles with time at 37 degrees C. The results of this study demonstrate that FESEM, coupled with sophisticated image analysis, may be an important tool in quantifying the distribution of aggregated species and assessing the overall stability of rAd samples.
Obenauer-Kutner
Linda J
LJ
Biotechnology Development, Schering-Plough Research Institute, Union, NJ 07083, USA. linda.obenauer-kutner@spcorp.com
Ihnat
Peter M
PM
Yang
Tong-Yuan
TY
Dovey-Hartman
Barbara J
BJ
Balu
Arthi
A
Cullen
Constance
C
Bordens
Ronald W
RW
Grace
Michael J
MJ
D000260:Adenoviruses, Human / Q000235:genetics / Q000648:ultrastructure*; D002138:Calibration; D036022:Capsid Proteins / Q000235:genetics / Q000502:physiology; D003673:Defective Viruses / Q000235:genetics / Q000648:ultrastructure*; D005434:Flow Cytometry; D016158:Genes, p53; D005822:Genetic Vectors / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D016624:Negative Staining; D010316:Particle Size; D013696:Temperature; D014771:Virion / Q000648:ultrastructure
D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus