Apoptosis and necrosis of hepatocytes induced in vitro by subeschar tissue fluid from guinea pigs with burn injury.
OBJECTIVE
To understand the effects of subeschar tissue fluid (STF) on postburn damage of the hepatocytes.


METHODS
In vitro cultured hepatocytes from normal guinea pigs were incubated with STF collected from guinea pigs with burn injury covering 30% total body surface area. Stained with Annexin-V-Fluos (A-V) and propidium iodide(PI), the hepatocytes were examined by flow cytometry for apoptosis and necrosis at both 12 and 24 h after incubation. The activities of alanine transaminase (ALT) and lactate dehydrogenase (LDH) in the culture medium were also assayed.


RESULTS
Incubation with STF induced apoptosis and necrosis of the hepatocytes and elevated activities of ALT and LDH in the culture medium.


CONCLUSIONS
STF induces apoptosis and necrosis of hepatocytes, which may play an important role in postburn organ damage.
Li
Zhi-Qing
ZQ
Department of Burns, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Zhou
Yi-Ping
YP
D000410:Alanine Transaminase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D001826:Body Fluids / Q000502:physiology*; D002056:Burns; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009336:Necrosis; D013997:Time Factors
D003470:Culture Media; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase