p23 and HSP20/alpha-crystallin proteins define a conserved sequence domain present in other eukaryotic protein families.
We identified families of proteins characterized by the presence of a domain similar to human p23 protein, which include proteins such as Sgt1, involved in the yeast kinetochore assembly; melusin, involved in specific interactions with the cytoplasmic integrin beta1 domain; Rar1, related to pathogenic resistance in plants, and to development in animals; B5+B5R flavo-hemo cytochrome NAD(P)H oxidoreductase type B in humans and mice; and NudC, involved in nucleus migration during mitosis. We also found that p23 and the HSP20/alpha-crystallin family of heat shock proteins, which share the same three-dimensional folding, show a pattern of conserved residues that points to a common origin in the evolution of both protein domains. The p23 and HSP20/alpha-crystallin phylogenetic relationship and their similar role in chaperone activity suggest a common function, probably involving protein-protein interaction, for those proteins containing p23-like domains.
Garcia-Ranea
J A
JA
Protein Design Group, Centro Nacional de Biotecnologia, 28049 Cantoblanco, Madrid, Spain. garcia@gredos.cnb.uam.es
Mirey
Gladys
G
Camonis
Jacques
J
Valencia
Alfonso
A
D000595:Amino Acid Sequence; D000818:Animals; D004331:Drosophila melanogaster / Q000737:chemistry / Q000378:metabolism*; D050886:HSP20 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D000071876:Prostaglandin-E Synthases; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D038201:alpha-Crystallins / Q000737:chemistry / Q000378:metabolism*
D050886:HSP20 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D010750:Phosphoproteins; D038201:alpha-Crystallins; C490849:PTGES3 protein, human; D000071876:Prostaglandin-E Synthases