Idiotypic network mimicry and antibody catalysis: lessons for the elicitation of efficient anti-idiotypic protease antibodies.
An important challenge in the field of catalytic antibodies is the generation of antibodies with designed sequence-specific protease activities. Such catalysts would not only be recruited for diverse applications in basic biological science, but could also offer new approaches in biotechnology and medicine. We have previously used the "internal image" property of the idiotypic network to elicit antibodies with efficient esterase and amidase activities. In the present report, we present preliminary results for the production of anti-idiotypic antibodies mimicking subtilisin. A monoclonal inhibitory antibody of subtilisin was characterized and used to elicit anti-idiotypic antibodies. Some of these antibodies exhibit not only an amidase activity against synthetic substrates, but are also able to cleave a protein, bovine serum albumin (BSA).
Pillet
Dominique
D
Génie Enzymatique et Cellulaire, UMR 6022 CNRS, Université de Technologie de Compiègne, BP 20529, Compiègne Cedex 60205, France.
Paon
Marjorie
M
Vorobiev
Ivan I
II
Gabibov
Alexander G
AG
Thomas
Daniel
D
Friboulet
Alain
A
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism*; D001665:Binding Sites; D010450:Endopeptidases / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D015842:Serine Proteinase Inhibitors / Q000276:immunology*; D013379:Substrate Specificity; D013381:Subtilisins / Q000037:antagonists & inhibitors / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D015842:Serine Proteinase Inhibitors; D010450:Endopeptidases; D013381:Subtilisins