title: Iron overload in the peritoneal cavity of women with pelvic endometriosis.
abstract: OBJECTIVE
  To examine the possible involvement of iron in the physiopathology of endometriosis.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Department of gynecology in a university hospital.
  
  
  METHODS
  Seventy patients undergoing laparoscopy.
  
  
  METHODS
  Collection of peritoneal fluid (n = 57), blood samples, and biopsy samples from endometrium (n = 62) and from endometriotic (n = 33) and normal-appearing peritoneum (n = 53).
  
  
  METHODS
  Measurement of iron and ferritin in serum and peritoneal fluid and staining of iron deposits with Prussian blue in tissues.
  
  
  RESULTS
  Iron and ferritin concentrations were significantly higher in the peritoneal fluid of patients with endometriosis compared with controls during the secretory phase. Higher rates of ferritin and hemosiderin deposits were observed in the peritoneum adjacent to red (100%), black (57%), and white (62%) lesions compared with normal-appearing peritoneum (25%). Deposits were more frequent during the secretory phase than the proliferative phase in healthy peritoneum from controls, whereas they were found throughout the cycle in the vicinity of lesions in patients with endometriosis. Similar rates of iron deposition were observed in the stroma of black and white lesions and in eutopic endometrium from patients with endometriosis.
  
  
  CONCLUSIONS
  Iron overload was observed in the cellular and peritoneal fluid compartments of the peritoneal cavity of women with endometriosis. Iron deposits seem to be related to the presence of lesions, suggesting that iron may be involved in the pathogenesis of endometriosis.
authors.affiliation: Department of Gynecology, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Van Langendonckt
authors.forename: Françoise
authors.initials: F
authors.lastname: Casanas-Roux
authors.forename: Jacques
authors.initials: J
authors.lastname: Donnez
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000737:chemistry; D001706:Biopsy; D004715:Endometriosis / Q000150:complications* / Q000378:metabolism; D004717:Endometrium / Q000737:chemistry; D005260:Female; D005293:Ferritins / Q000032:analysis* / Q000097:blood; D005295:Ferrocyanides; D006801:Humans; D007501:Iron / Q000032:analysis* / Q000097:blood; D019190:Iron Overload / Q000150:complications / Q000175:diagnosis*; D017737:Macrophages, Peritoneal / Q000737:chemistry; D010537:Peritoneum / Q000737:chemistry*; D011446:Prospective Studies; D013194:Staining and Labeling
chemical_list: D005295:Ferrocyanides; D005293:Ferritins; D007501:Iron; C000170:ferric ferrocyanide