title: Field evaluation of a second-generation cytometer UF-100 in diagnosis of acute urinary tract infections in adult patients.
abstract: OBJECTIVE
  The authors evaluated the analytical performance of the Sysmex UF-100 cytometer vs. the diagnosis of urinary tract infections (UTI).
  
  
  METHODS
  We considered 2010 subjects, aged between 18 and 78, 870 males and 1140 females. The majority (90.2%) of the samples were voided urine specimens collected by using the midstream technique. Each sample was subjected to microbiological evaluation (culture + residual antibacterial activity), dipstick tests, UF-100 examination and microscopic observation. In order to obtain a final diagnosis of UTI these laboratory results were taken into consideration together with clinical data and patients' characteristics. The analytical performance of the laboratory tests was obtained by adopting this diagnosis as standard practice.
  
  
  RESULTS
  Out of the total 2010 subjects considered a clinical diagnosis of UTI was obtained in 529 cases (26.32%). The UF-100-based screening had sensitivity, 0.94; specificity, 0.93; positive predictive value, 0.83; negative predictive value, 0.98; and correctly classified incidence, 0.93.
  
  
  CONCLUSIONS
  In our experience the results of the UF-100-based screening show a very good correlation with the diagnosis of acute UTI in adults patients.
authors.affiliation: Clinical Pathology Department, ASL 14, Civil Hospital, Via Madonna Marina 500, 30019 Chioggia (Venice), Italy.
authors.forename: F
authors.initials: F
authors.lastname: Manoni
authors.forename: S
authors.initials: S
authors.lastname: Valverde
authors.forename: F
authors.initials: F
authors.lastname: Antico
authors.forename: M M
authors.initials: MM
authors.lastname: Salvadego
authors.forename: A
authors.initials: A
authors.lastname: Giacomini
authors.forename: G
authors.initials: G
authors.lastname: Gessoni
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D003470:Culture Media; D004909:Erythrocyte Indices / Q000502:physiology; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008297:Male; D015995:Prevalence; D012680:Sensitivity and Specificity; D014552:Urinary Tract Infections / Q000175:diagnosis*
chemical_list: D003470:Culture Media