Field testing of Schistosoma japonicum DNA vaccines in cattle in China.
Vaccines are needed to reduce the zoonotic reservoir of Schistosoma japonicum infection in bovines in China. We have developed two experimental DNA vaccines and have already shown these to be capable of inducing partial protection in water buffalo naturally exposed to the risk of S. japonicum infection in the field. We now report a similar field trial in cattle, the other major bovine reservoir host species in China. Groups of cattle were vaccinated with the VRSj28 vaccine or the VRSj23 vaccine, or, to test whether protection could be enhanced by combination vaccination, with both these DNA vaccines together. After vaccination, the cattle were exposed to natural infection in the field for a period of 54 days. Worm and egg counts carried out at the end of the experiment showed that each of the vaccine groups showed partial resistance, and that combined vaccination was not more effective than vaccination with the individual plasmids.
Shi
Fuhui
F
Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, 3 Lane 345 Shi-long Road, Shanghai 200232, PR China.
Zhang
Yaobi
Y
Lin
Jiaojiao
J
Zuo
Xin
X
Shen
Wei
W
Cai
Yiumin
Y
Ye
Ping
P
Bickle
Quentin D
QD
Taylor
Martin G
MG
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D002020:Buffaloes / Q000469:parasitology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000469:parasitology / Q000517:prevention & control*; D002681:China; D005260:Female; D007115:Immunization Schedule; D008297:Male; D010270:Parasite Egg Count / Q000379:methods / Q000706:statistics & numerical data; D012549:Schistosoma japonicum / Q000276:immunology* / Q000302:isolation & purification; D012554:Schistosomiasis japonica / Q000175:diagnosis / Q000517:prevention & control* / Q000662:veterinary*; D017778:Vaccines, Combined / Q000627:therapeutic use; D019444:Vaccines, DNA / Q000627:therapeutic use*; D014614:Vaccines, Synthetic / Q000627:therapeutic use
D000909:Antibodies, Helminth; D017778:Vaccines, Combined; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic