title: Genetics of isoprenoid biosynthesis in Paracoccus zeaxanthinifaciens.
abstract: The genes coding for all the enzymes involved in the conversion of acetyl-CoA to farnesyl diphosphate (FPP) in the zeaxanthin-producing bacterium Paracoccus zeaxanthinifaciens were cloned and characterized. Two genes encoding enzymes catalysing the condensation of two acetyl-CoA molecules to acetoacetyl-CoA were found. The six enzymes involved in the conversion of acetyl-CoA and acetoacetyl-CoA to isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) are grouped in an operon, designated the mevalonate operon. The gene encoding the enzyme catalysing two consecutive condensations, IPP and DMAPP to geranyl diphosphate (GPP) and IPP and GPP to FPP, is not clustered with any other gene encoding an enzyme of the isoprenoid pathway. Genes encoding enzymes involved in the biosynthesis of poly-hydroxyalkanoate and non-carotenoid isoprenoids found in P. zeaxanthinifaciens are also presented.
authors.affiliation: Biotechnology Department, Roche Vitamins AG, CH-4070, Basel, Switzerland. markus.huembelin@roche.com
authors.forename: Markus
authors.initials: M
authors.lastname: Hümbelin
authors.forename: Ann
authors.initials: A
authors.lastname: Thomas
authors.forename: Junying
authors.initials: J
authors.lastname: Lin
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Jan
authors.initials: J
authors.lastname: Jore
authors.forename: Alan
authors.initials: A
authors.lastname: Berry
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism; D000214:Acyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D019748:Carbon-Carbon Double Bond Isomerases / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D045782:Hemiterpenes; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics; D008565:Membrane Proteins; D008798:Mevalonic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000378:metabolism; D010230:Paracoccus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012717:Sesquiterpenes; D013729:Terpenes / Q000378:metabolism*
chemical_list: D000214:Acyl Coenzyme A; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D045782:Hemiterpenes; C101292:IspA protein, Shigella flexneri; D008565:Membrane Proteins; D009943:Organophosphorus Compounds; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; D013729:Terpenes; C010667:acetoacetyl CoA; C004809:isopentenyl pyrophosphate; D000105:Acetyl Coenzyme A; C004808:farnesyl pyrophosphate; D006903:Hydroxymethylglutaryl CoA Reductases; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase; D008798:Mevalonic Acid