Voltammetric studies of the interaction of transition-metal complexes with DNA.
The interaction of the two new synthesized transition-metal complexes, ML(2) (M=Co, Cu, L=1,8-dihydroxyethyl-1, 3,8,10,13-hexa-azacyclotetradecane) with calf thymus DNA was probed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Adding deoxyribonucleic acid (DNA) into [CoL](2+) and [CuL](2+) solution, the i(p) value of all the peaks of [CoL](2+) and [CuL](2+) significantly decreased in proportion to concentration of DNA. Glassy carbon electrodes (GCEs) were modified with DNA by adsorption, and it was electrochemically characterized with transition-metal complexes, [ML](2+). The DNA modification layer on the GCE is unstable to alkali and to heat, but stable to acid solutions and very stable in long stock in a dry state. It could be seen that peak potential shifted positively and the peak current increased significantly. The electrochemical parameters, binding constant (k(n+)) and binding sites(s) were calculated by a nonlinear regression method.
Lu
Xiaoquan
X
Department of Chemistry, Northwest Normal University, Lanzhou 730070, Gansu, China. xqlu88@nwnu.edu.cn
Zhu
Kaimei
K
Zhang
Min
M
Liu
Hongde
H
Kang
Jingwan
J
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003035:Cobalt / Q000032:analysis / Q000737:chemistry; D003300:Copper / Q000032:analysis / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D008670:Metals / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry; D017186:Titrimetry / Q000379:methods*; D028561:Transition Elements / Q000032:analysis* / Q000737:chemistry*
D008670:Metals; D010618:Phenanthrolines; D028561:Transition Elements; D003035:Cobalt; D003300:Copper; D004247:DNA; C025205:1,10-phenanthroline