Immunohistochemical labeling of normal melanocytes.
Comparison of seven antibodies for the demonstration of normal melanocytes in formalin-fixed, paraffin-embedded surgical discard skin showed that the monoclonal antibody Mel-5 (clone TA99) directed against pigment associated antigen was the most sensitive. Quantitative data were obtained for the sensitivity of the antibodies NKI/beteb, S100, T311, Melan A (clone A103), c-kit, and Mel-5 in parallel sections of human skin. An anticytokeratin antibody (CK34betaE12) was also used to stain basal keratinocytes and provide a negative image of the melanocytes present. Optimal conditions for the use of Mel-5 in paraffin sections of skin are described.
Dean
Nicola R
NR
Co-operative Research Centre for Tissue Growth and Repair, Child Health Research Institute, Department of Plastic and Reconstructive Surgery, Royal Adelaide Hospital and University of Adelaide, Adelaide, Australia.
Brennan
James
J
Haynes
Julie
J
Goddard
Christopher
C
Cooter
Rodney D
RD
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D005260:Female; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods* / Q000706:statistics & numerical data; D015603:Keratinocytes / Q000276:immunology / Q000378:metabolism; D007633:Keratins / Q000276:immunology / Q000378:metabolism; D058965:MART-1 Antigen; D008544:Melanocytes / Q000276:immunology / Q000378:metabolism*; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000276:immunology / Q000378:metabolism; D009418:S100 Proteins / Q000276:immunology / Q000378:metabolism; D012680:Sensitivity and Specificity; D014746:Vimentin / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; C121278:CK-34 beta E12; D006023:Glycoproteins; D058965:MART-1 Antigen; C094218:MEL-5 protein, human; C548667:MLANA protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D009418:S100 Proteins; D014746:Vimentin; D007633:Keratins; D019009:Proto-Oncogene Proteins c-kit