Potentiation of serum response factor activity by a family of myocardin-related transcription factors.
Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac and smooth muscle cells, MRTF-A and -B are expressed in numerous embryonic and adult tissues. In SRF-deficient embryonic stem cells, myocardin and MRTFs are unable to activate SRF-dependent reporter genes, confirming their dependence on SRF. Myocardin and MRTFs comprise a previously uncharacterized family of SRF cofactors with the potential to modulate SRF target genes in a wide range of tissues.
Wang
Da-Zhi
DZ
Department of Molecular Biology, University of Texas, Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas 75390-9148, USA.
Li
Shijie
S
Hockemeyer
Dirk
D
Sutherland
Lillian
L
Wang
Zhigao
Z
Schratt
Gerhard
G
Richardson
James A
JA
Nordheim
Alfred
A
Olson
Eric N
EN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D018507:Gene Expression Regulation, Developmental; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D026362:Serum Response Factor / Q000235:genetics* / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors; D014981:Xenopus; D000099251:Myocardin
D018076:DNA, Complementary; D005982:Glutathione Transferase; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D026362:Serum Response Factor; D015534:Trans-Activators; D014157:Transcription Factors; D000099251:Myocardin