title: Transcription through the iab-7 cis-regulatory domain of the bithorax complex interferes with maintenance of Polycomb-mediated silencing.
abstract: The Fab-7 chromatin domain boundary insures functional autonomy of the iab-6 and iab-7 cis-regulatory domains in the bithorax complex (BX-C). We have previously shown that chromatin insulators such as gypsy or scs(min) are potent insulators that cannot substitute for Fab-7 function within the BX-C. During the early stages of these swapping experiments, we initially used a fragment of scs that was slightly larger than a minimal scs element (scs(min)). We report that this scs fragment, unlike scs(min), interferes in an orientation-dependent manner with the output of a regulatory region covering 80 kb of DNA (from iab-4 to iab-8). At the core of this orientation-dependent phenotype is a promoter located immediately adjacent to the scs insulator. In one orientation, the promoter traps the activity of the iab-3 through iab-5 cis-regulatory domains, diverting them from the abd-A gene. In the opposite orientation, the promoter is transcribing the iab-7 cis-regulatory domain, resulting in ectopic activation of the latter. Our data suggest that transcription through a Polycomb-Response Element (PRE) interferes with the maintenance of a Polycomb repression complex.
authors.affiliation: Department of Zoology and Animal Biology, University of Geneva, 30 quai E. Ansermet, Switzerland.
authors.forename: Ilham
authors.initials: I
authors.lastname: Hogga
authors.forename: François
authors.initials: F
authors.lastname: Karch
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development*; D029721:Drosophila Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing; D005801:Genes, Homeobox; D017344:Genes, Insect; D005809:Genes, Regulator; D005810:Multigene Family; D063150:Polycomb Repressive Complex 1; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D029721:Drosophila Proteins; C066770:Pc protein, Drosophila; D063150:Polycomb Repressive Complex 1