title: Bovine gamma delta T cell subsets express distinct patterns of chemokine responsiveness and adhesion molecules: a mechanism for tissue-specific gamma delta T cell subset accumulation.
abstract: Subsets of gammadelta T cells localize to distinct tissue sites in the absence of exogenous Ag stimulation or development of effector/memory cells. Selective lymphocyte homing from the blood into tissues is controlled by a multistep process involving vascular and lymphocyte adhesion molecules, and G protein-linked chemokine receptors. The role of these mechanisms in the tissue tropism of gammadelta T cells is still poorly understood. In this study, we demonstrate that a subset of gammadelta T cells, most of which express an antigenically distinct TCR and are characterized by coexpression of CD8, selectively accumulated in tissues that expressed high levels of the mucosal vascular addressin, mucosal addressin cell adhesion molecule 1. These cells expressed higher levels of alpha(4)beta(7) integrins than other gammadelta T cell subsets and selectively migrated to the CCR7 ligand secondary lymphoid-tissue chemokine (CCL21). Integrin activation by CCL21 selectively increased CD8(+)gammadelta T cell binding to recombinant mucosal addressin cell adhesion molecule 1. These results suggest that the tropism of circulating CD8(+)gammadelta T cells for mucosal tissues is due, at least in part, to selective developmental expression of adhesion molecules and chemokine receptors.
authors.affiliation: Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University School of Medicine, and Center for Molecular Biology and Medicine, Veterans Affairs Palo Alto Health Care System, CA 94304, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Wilson
authors.forename: Jodi F
authors.initials: JF
authors.lastname: Hedges
authors.forename: Eugene C
authors.initials: EC
authors.lastname: Butcher
authors.forename: Michael
authors.initials: M
authors.lastname: Briskin
authors.forename: Mark A
authors.initials: MA
authors.lastname: Jutila
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D016466:CHO Cells; D002417:Cattle; D002448:Cell Adhesion / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D019742:Chemokines, CC / Q000378:metabolism / Q000502:physiology*; D002634:Chemotaxis, Leukocyte / Q000235:genetics / Q000276:immunology*; D006224:Cricetinae; D005434:Flow Cytometry; D007136:Immunoglobulins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D039441:Integrin alpha4 / Q000096:biosynthesis; D039641:Integrin beta Chains / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D009088:Mucoproteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D009928:Organ Specificity / Q000276:immunology; D011485:Protein Binding / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000097:blood / Q000502:physiology*; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D011994:Recombinant Proteins / Q000378:metabolism; D012995:Solubility; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D014162:Transfection
chemical_list: C515461:Ccl19 protein, mouse; C515479:Ccl21c protein, mouse; C515371:Ccr7 protein, mouse; D015815:Cell Adhesion Molecules; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D019742:Chemokines, CC; D007136:Immunoglobulins; D039641:Integrin beta Chains; C489082:Madcam1 protein, mouse; D009088:Mucoproteins; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta; D054400:Receptors, CCR7; D019707:Receptors, Chemokine; D011994:Recombinant Proteins; C091368:integrin beta7; D039441:Integrin alpha4