Downregulation of voltage-gated potassium channel alpha gene expression in dorsal root ganglia following chronic constriction injury of the rat sciatic nerve.
The hyperexcitability and ectopic spontaneous discharge (ESD) of primary sensory neurons may be important for the generation or maintenance of neuropathic pain. To investigate the relationship between the electrical abnormalities of injured neurons and voltage-gated potassium (Kv) channel gene expression, the expression of the Kv channel alpha genes in the dorsal root ganglion (DRG) was monitored by reverse transcription-polymerase chain reaction (RT-PCR) in a chronic constriction injury (CCI) model of neuropathic pain. Electrophoresis of the RT-PCR products showed the presence of several Kv alpha transcript types with various levels of basal expression in lumbar 4, 5, and 6 DRGs. The Kv 1.2, 1.4, 2.2, 4.2, and 4.3 mRNA levels in the ipsilateral DRG were 63-73% of the contralateral sides of the same animal at 3 days and 34-63% at 7 days following CCI. In addition, Kv 1.1 mRNA levels declined to about 72% of the contralateral level at 7 days. No significant changes in Kv 1.5, 1.6, 2.1, 3.1, 3.2, 3.5, and 4.1 mRNA levels were detectable in the ipsilateral DRG at both days. These results suggest that the downregulation of Kv channel alpha gene expression in the DRG following CCI may result in the reduction of K(+) current and contribute to neuronal excitability and ESD generation.
Kim
Dong Sun
DS
Department of Anatomy, School of Medicine, Kyungpook National University, 2-101 Dongin Dong, Taegu 700-422, South Korea.
Choi
Jeong Ock
JO
Rim
Hyo Deog
HD
Cho
Hee Jung
HJ
D000200:Action Potentials / Q000235:genetics; D000818:Animals; D002908:Chronic Disease; D015536:Down-Regulation / Q000235:genetics*; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000503:physiopathology; D005786:Gene Expression Regulation / Q000235:genetics; D051663:Kv1.2 Potassium Channel; D008297:Male; D009431:Neural Conduction / Q000235:genetics; D009437:Neuralgia / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D009475:Neurons, Afferent / Q000378:metabolism*; D010523:Peripheral Nervous System Diseases / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D015221:Potassium Channels / Q000235:genetics*; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism / Q000601:surgery
C496972:Kcna2 protein, rat; D051663:Kv1.2 Potassium Channel; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger