title: A biotechnological process involving filamentous fungi to produce natural crystalline vanillin from maize bran.
abstract: A new process involving the filamentous fungi Aspergillus niger and Pycnoporus cinnabarinus has been designed for the release of ferulic acid by enzymic degradation of a cheap and natural agricultural byproduct (autoclaved maize bran) and its biotransformation into vanillic acid and/or vanillin with a limited number of steps. On the one hand, the potentialities of A. niger I-1472 to produce high levels of polysaccharide-degrading enzymes including feruloyl esterases and to transform ferulic acid into vanillic acid were successfully combined for the release of free ferulic acid from autoclaved maize bran. Then vanillic acid was recovered and efficiently transformed into vanillin by P. cinnabarinus MUCL39533, since 767 mg/L of biotechnologic vanillin could be produced in the presence of cellobiose and XAD-2 resin. On the other hand, 3-d-old high-density cultures of P. cinnabarinus MUCL39533 could be fed with the autoclaved fraction of maize bran as a ferulic acid source and A. niger I-1472 culture filtrate as an extracellular enzyme source. Under these conditions, P. cinnabarinus MUCL39533 was shown to directly biotransform free ferulic acid released from the autoclaved maize bran by A. niger I-1472 enzymes into 584 mg/L of vanillin. These processes, involving physical enzymic, and fungal treatments, permitted us to produce crystallin vanillin from autoclaved maize bran without any purification step.
authors.affiliation: Unité de Biotechnologie des Champignons Filamenteux de l'INRA, IFR de Biotechnologie Agro-Industrielle de Marseille, France. lesage@esil.univ-mrs.fr
authors.forename: Laurence
authors.initials: L
authors.lastname: Lesage-Meessen
authors.forename: Anne
authors.initials: A
authors.lastname: Lomascolo
authors.forename: Estelle
authors.initials: E
authors.lastname: Bonnin
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Thibault
authors.forename: Alain
authors.initials: A
authors.lastname: Buleon
authors.forename: Marc
authors.initials: M
authors.lastname: Roller
authors.forename: Michele
authors.initials: M
authors.lastname: Asther
authors.forename: Eric
authors.initials: E
authors.lastname: Record
authors.forename: Benoit Colonna
authors.initials: BC
authors.lastname: Ceccaldi
authors.forename: Marcel
authors.initials: M
authors.lastname: Asther
mesh_terms: D001234:Aspergillus niger / Q000378:metabolism*; D001487:Basidiomycota / Q000378:metabolism*; D001547:Benzaldehydes / Q000737:chemistry / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D003373:Coumaric Acids / Q000378:metabolism; D003460:Crystallization; D006867:Hydrolases / Q000378:metabolism; D014641:Vanillic Acid / Q000378:metabolism; D014961:X-Ray Diffraction; D003313:Zea mays / Q000378:metabolism*
chemical_list: D001547:Benzaldehydes; D003373:Coumaric Acids; C004999:ferulic acid; C100058:vanillin; D006867:Hydrolases; D014641:Vanillic Acid