title: Genetic analysis of de novo CD5+ diffuse large B-cell lymphomas suggests an origin from a somatically mutated CD5+ progenitor B cell.
abstract: CD5(+) diffuse large B-cell lymphomas (DLBLs) have recently been described as a particular subgroup of DLBLs. Classical banding and interphase cytogenetic analyses targeting ATM, TP53, and P16(INK4a) genes and the D13S25 locus from 13 CD5(+) DLBLs were compared with 55 CD5(-) DLBLs. Additionally, analysis of somatic mutations of the immunoglobulin heavy chain variable region (IgVH) genes were performed in CD5(+) DLBLs. CD5(+) DLBLs were somatically mutated (7 of 8 cases) and were negative for t(11;14)(q13;q32) and t(14;18)(q32;q21), whereas t(3;14)(q27;q32) was found in only one tumor. Trisomy 3 and gains on chromosomes 16/16p and 18/18q were significantly overrepresented in CD5(+) DLBLs. No ATM deletions were detected. The prevalence of deletions at the D13S25 locus was significantly higher in CD5(+) DLBLs (4 of 12 [33%]) compared with CD5(-) DLBLs (4 of 42 [10%]), as were p16(INK4a) deletions (33% versus 8%). On the basis of these findings, CD5(+) DLBLs are likely to arise from the same progenitor cell as the mutated variant of CD5(+) lymphocytic lymphoma/B-cell chronic lymphocytic leukemia (B-CLL).
authors.affiliation: Institute of Pathology, University of Würzburg, Department of Medicine III, University Hospital Grosshadern/LMU München, Germany. path054@mail.uni-wuerzburg.de
authors.forename: Tiemo
authors.initials: T
authors.lastname: Katzenberger
authors.forename: Andreas
authors.initials: A
authors.lastname: Lohr
authors.forename: Stephan
authors.initials: S
authors.lastname: Schwarz
authors.forename: Martin
authors.initials: M
authors.lastname: Dreyling
authors.forename: Julia
authors.initials: J
authors.lastname: Schoof
authors.forename: Christina
authors.initials: C
authors.lastname: Nickenig
authors.forename: Stephan
authors.initials: S
authors.lastname: Stilgenbauer
authors.forename: Jörg
authors.initials: J
authors.lastname: Kalla
authors.forename: M Michaela
authors.initials: MM
authors.lastname: Ott
authors.forename: Hans Konrad
authors.initials: HK
authors.lastname: Müller-Hermelink
authors.forename: German
authors.initials: G
authors.lastname: Ott
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D018956:CD5 Antigens; D019070:Cell Lineage; D020732:Cytogenetic Analysis; D005803:Genes, Immunoglobulin; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D016393:Lymphoma, B-Cell / Q000209:etiology / Q000235:genetics / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D009154:Mutation
chemical_list: D018956:CD5 Antigens; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region