title: Determination of biomass detachment rate coefficients in anaerobic fluidized-bed-GAC reactors.
abstract: In this study a simple analytical technique that determines the active biomass in a sample capable of degrading specific substrates anaerobically was developed and used to measure first-order detachment rate coefficients of acetate and phenol utilizing organisms. Different detachment rate coefficients were measured for the two microbial populations. A general relationship between the detachment rate coefficients and biomass accumulation was developed. The detachment rate coefficient was found to increase sharply after a certain biomass accumulation until it reaches a maximum, after which it decreases continuously with increasing biomass until it levels off at a constant value independent of biomass. Detachment rate coefficients measured in different reactors, operating at different conditions with respect to loading, biological SRT, and attachment media particle size, were in close agreement with each other after being normalized to the shear stress and daily methane production rate per unit biofilm area.
authors.affiliation: Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario, Canada N6A 5B9.
authors.forename: George
authors.initials: G
authors.lastname: Nakhla
authors.forename: Makram T
authors.initials: MT
authors.lastname: Suidan
mesh_terms: D000085:Acetates / Q000378:metabolism; D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000502:physiology*; D001422:Bacterial Adhesion / Q000502:physiology*; D018441:Biofilms; D018533:Biomass; D019149:Bioreactors; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D007220:Industrial Waste; D008697:Methane / Q000378:metabolism; D010316:Particle Size; D019800:Phenol / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D033081:Shear Strength; D013045:Species Specificity; D013314:Stress, Mechanical; D013499:Surface Properties; D014865:Waste Disposal, Fluid / Q000379:methods; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D000085:Acetates; D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D019800:Phenol; D008697:Methane