title: Adhesion of normal erythrocytes at depressed venous shear rates to activated neutrophils, activated platelets, and fibrin polymerized from plasma.
abstract: Deep vein thrombosis (DVT) is a low flow pathology often prevented by vascular compression to increase blood movement. We report new heterotypic adhesive interactions of normal erythrocytes operative at low wall shear rates (gamma(w)) below 100 s(-1). Adhesion at gamma(w) = 50 s(-1) of washed red blood cells (RBCs) to fibrinogen-adherent platelets was 4-fold less (P <.005) than to collagen-adherent platelets (279 +/- 105 RBC/mm(2)). This glycoprotein VI (GPVI)-triggered adhesion was antagonized (> 80% reduction) by soluble fibrinogen (3 mg/mL) and ethylenediaminetetraacetic acid (EDTA). RBC-platelet adhesion was reduced in half by antibodies against CD36 or GPIb, but not by antibodies against GPIIb/IIIa, von Willebrand factor (VWF), thrombospondin (TSP), P-selectin, beta(1), alpha(v), or CD47. Adhesion of washed RBCs to fibrinogen-adherent neutrophils was increased 6-fold in the presence of 20 microM N-formyl-Met-Leu-Phe to a level of 67 RBCs per 100 neutrophils after 5 minutes at 50 s(-1). RBC-neutrophil adhesion was diminished by anti-CD11b (76%), anti-RBC Landsteiner-Wiener (LW) (ICAM4; 40%), or by EDTA (> 80%), but not by soluble fibrinogen or antibodies against CD11a, CD11c, CD36, TSP, beta(1), alpha(v), or CD47. RBC adhesion to activated platelets and activated neutrophils was prevented by wall shear stress above 1 dyne/cm(2) (at 100 s(-1)). Whereas washed RBCs did not adhere to fibrin formed from purified fibrinogen, adhesion was marked when pure fibrin was precoated with TSP or when RBCs were perfused over fibrin formed from recalcified plasma. Endothelial activation and unusually low flow may be a setting prone to receptor-mediated RBC adhesion to adherent neutrophils (or platelets/fibrin), all of which may contribute to DVT.
authors.affiliation: Department of Chemical Engineering, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia 19104, USA.
authors.forename: Mukul S
authors.initials: MS
authors.lastname: Goel
authors.forename: Scott L
authors.initials: SL
authors.lastname: Diamond
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology*; D005337:Fibrin / Q000378:metabolism; D018056:Hemorheology; D006487:Hemostasis; D006801:Humans; D018715:Microscopy, Video; D018375:Neutrophil Activation; D009504:Neutrophils / Q000166:cytology / Q000502:physiology; D019007:P-Selectin / Q000494:pharmacology; D010949:Plasma; D015539:Platelet Activation; D013314:Stress, Mechanical; D020246:Venous Thrombosis / Q000209:etiology
chemical_list: D019007:P-Selectin; D005337:Fibrin