Responses of IgM for enterovirus 71 infection.
A rapid serological assay was developed for detection of specific IgM to enterovirus 71, a human picornavirus that is usually associated with severe central nervous system complications. The sensitivity and specificity of this "in-house" mu-capture enzyme linked-immunosorbent assay was assessed by testing 213 serum samples. With the conventional virus culture as a standard method, the sensitivity and specificity were 91.5 and 93.1%, respectively, for this newly developed immunoassay. This method allows for detection of the IgM responses from the patients either infected by genotype B or genotype C of enterovirus 71. IgM can be detected as early as the second day from the onset of disease. IgM responses exhibit 100% positive rate from enterovirus 71-infected patients with complications, including encephalitis, meningitis, polio-like syndrome, pulmonary edema, and fatal cases. These findings suggest that detection of specific IgM by the use of enzyme linked-immunosorbent assay is a rapid and valuable way for the diagnosis of enterovirus 71 infection.
Tsao
Kuo-Chien
KC
School of Medical Technology, Chang Gung University, Tao-Yuan, Taiwan.
Chan
Err-Cheng
EC
Chang
Luan-Yin
LY
Chang
Pi-Yueh
PY
Huang
Chung-Guei
CG
Chen
Yu-Ping
YP
Chang
Shih-Cheng
SC
Lin
Tzou-Yien
TY
Sun
Chien-Fen
CF
Shih
Shin-Ru
SR
D000914:Antibodies, Viral / Q000032:analysis*; D004770:Enterovirus / Q000276:immunology / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D012680:Sensitivity and Specificity; D012703:Serotyping
D000914:Antibodies, Viral; D007075:Immunoglobulin M