Jak3-regulated genes: DNA array analysis of concanavalin a-interleukin-2-activated chicken T cells treated with a specific jak3 inhibitor.
Janus kinase 3 (Jak3) is important in the activation and proliferation of lymphoid cells and binds to the common gamma subunit of several cytokine receptors, including the interleukin-2 (IL-2) receptor (IL-2R). DNA arrays were used to measure mRNA levels of a large number of genes regulated by signaling through the Jak3 tyrosine kinase pathway by blocking concanavalin A (ConA)-IL-2-activated chicken splenic T cells with a specific Jak3 inhibitor (WHI-P154). Of the 635 genes detected by arrays containing about 1200 cDNAs, 12 were upregulated in control cells compared with inhibitor-treated cells, and 6 were expressed at higher levels in the inhibitor-treated group. By identifying genes that are directly or indirectly regulated by Jak3, we can gain insight into the roles of this key intermediate in avian T cell activation and further our understanding of intracellular signaling networks in the immune response.
Kampa
Dione
D
Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19711, USA.
Burnside
Joan
J
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D002645:Chickens; D003208:Concanavalin A / Q000494:pharmacology*; D018076:DNA, Complementary / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D015723:Gene Library; D007376:Interleukin-2 / Q000494:pharmacology*; D053616:Janus Kinase 3; D008213:Lymphocyte Activation / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation / Q000187:drug effects; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000502:physiology*; D011799:Quinazolines / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism
D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D007376:Interleukin-2; D011799:Quinazolines; D011994:Recombinant Proteins; C113888:WHI P154; D003208:Concanavalin A; D011505:Protein-Tyrosine Kinases; D053616:Janus Kinase 3