title: In vivo gene transfer of hepatocyte growth factor to skeletal muscle prevents changes in rat kidneys after 5/6 nephrectomy.
abstract: Hepatocyte growth factor (HGF) has been reported to be a renal regeneration factor. We previously reported that HGF acts as a renotropic factor, inducing cell recovery from ischemic injury or drug toxicity. Gene transfer by electroporation, which uses plasmid DNA as the vector, has several advantages over the conventional gene transfer method using viral vectors, inducing the ability to perform repeated transfers without apparent immunologic responses to the DNA vector. We recently demonstrated that electroporation of the HGF gene into skeletal muscle was an effective treatment for liver injury in an animal model. We presently investigated prevention of development of chronic renal disease by repetitive HGF gene transfer in rats with 5/6 nephrectomy. Hepatocyte growth factor gene transfer-treated rats showed better growth in body weight than untreated rats. Histologic changes such as glomerulosclerosis and interstitial fibrosis were significantly ameliorated by HGF gene transfer compared with untreated rats. Hepatocyte growth factor gene transfer by electroporation into skeletal muscle is feasible and effective against morphologic injury in subtotally nephrectomized rats.
authors.affiliation: Department of Urology, Gunma University School of Medicine, Maebashi, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Naotsugu
authors.initials: N
authors.lastname: Ichimaru
authors.forename: Shiro
authors.initials: S
authors.lastname: Takahara
authors.forename: Koji
authors.initials: K
authors.lastname: Yazawa
authors.forename: Motoaki
authors.initials: M
authors.lastname: Hatori
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Isaka
authors.forename: Toshiki
authors.initials: T
authors.lastname: Moriyama
authors.forename: Enyu
authors.initials: E
authors.lastname: Imai
authors.forename: Haruhito
authors.initials: H
authors.lastname: Azuma
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Nakamura
authors.forename: Akihiko
authors.initials: A
authors.lastname: Okuyama
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Yamanaka
mesh_terms: D000818:Animals; D001835:Body Weight; D018274:Electroporation; D018014:Gene Transfer Techniques; D017228:Hepatocyte Growth Factor / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000378:metabolism* / Q000601:surgery; D008264:Macrophages / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D009392:Nephrectomy; D051381:Rats
chemical_list: D017228:Hepatocyte Growth Factor