The analysis of the fine specificity of celiac disease antibodies using tissue transglutaminase fragments.
Celiac disease is an intestinal malabsorption characterized by an intolerance to cereal proteins accompanied by immunological responses to dietary gliadins and an autoantigen located in the endomysium. The latter has been identified as the enzyme tissue transglutaminase which belongs to a family of enzymes that catalyze protein cross-linking reactions and is constitutively expressed in many tissues as well as being activated during apoptosis. In a recent paper, we described the selection and characterization of anti-transglutaminase Igs from phage antibody libraries created from intestinal lymphocytes from celiac disease patients. In this work, using transglutaminase gene fragments, we identify a region of tissue transglutaminase recognized by these antibodies as being conformational and located in the core domain of the enzyme. This is identical to the region recognized by anti-transglutaminase Igs found in the serum of celiac disease patients.
Sblattero
Daniele
D
Dipartimento di Biologia, University of Trieste, Trieste, Italy. daniele@icgeb.org
Florian
Fiorella
F
Azzoni
Elisabetta
E
Zyla
Trevin
T
Park
Min
M
Baldas
Valentina
V
Not
Tarcisio
T
Ventura
Alessandro
A
Bradbury
Andrew
A
Marzari
Roberto
R
D000918:Antibody Specificity / Q000502:physiology*; D001323:Autoantibodies / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002446:Celiac Disease / Q000276:immunology*; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D019204:GTP-Binding Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011487:Protein Conformation; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D017434:Protein Structure, Tertiary / Q000502:physiology; D011503:Transglutaminases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D001323:Autoantibodies; D010446:Peptide Fragments; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins