Fos expression in non-catecholaminergic neurons in medullary and pontine nuclei after volume depletion induced by polyethylene glycol.
Fos immunocytochemistry was combined with immunolabeling for dopamine-beta-hydroxylase (DBH) to examine neuronal activation in the medulla and pons after administration of polyethelene glycol (PEG), which produces volume depletion without altering arterial blood pressure. Increased Fos immunoreactivity was observed in the area postrema, nucleus of the solitary tract, rostral ventrolateral medulla, lateral parabrachial nucleus, and the dorsomedial pons at the level of the locus coeruleus. Fos immunolabeling in the caudal nucleus of the solitary tract, rostral ventrolateral medulla, and the dorsomedial pons occurred primarily in neurons that did not contain DBH. Thus, PEG activates non-catecholaminergic neurons in medullary and pontine areas associated with cardiovascular and body fluid regulation.
Curtis
Kathleen S
KS
Department of Psychology and Neuroscience Program, Florida State University, Tallahassee, FL 32306-1270, USA. curtis@psy.fsu.edu
Krause
Eric G
EG
Contreras
Robert J
RJ
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001810:Blood Volume / Q000187:drug effects; D004299:Dopamine beta-Hydroxylase / Q000096:biosynthesis; D007150:Immunohistochemistry; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011149:Pons / Q000166:cytology / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017552:Solitary Nucleus / Q000166:cytology / Q000378:metabolism
D016760:Proto-Oncogene Proteins c-fos; D011092:Polyethylene Glycols; D004299:Dopamine beta-Hydroxylase