The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is involved in L-ascorbate metabolism in Escherichia coli.
Mutations in yjfQ allowed us to identify this gene as the regulator of the operon yjfS-X (ula operon), reported to be involved in L-ascorbate metabolism. Inactivation of this gene renders constitutive the expression of the ula operon, indicating that YjfQ acts as a repressor. We also demonstrate that this repressor regulates the nearby yjfR gene, which in this way constitutes a regulon with the ula operon.
Campos
Evangelina
E
Department of Biochemistry, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, Spain.
Aguilar
Juan
J
Baldoma
Laura
L
Badia
Josefa
J
D000595:Amino Acid Sequence; D001205:Ascorbic Acid / Q000378:metabolism*; D001483:Base Sequence; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D009876:Operon; D018085:Regulon; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
D029968:Escherichia coli Proteins; D012097:Repressor Proteins; C467899:YifQ protein, E coli; D001205:Ascorbic Acid