Visual immunoprecipitate assay eight hour method for detection of enterohemorrhagic Escherichia coli O157:H7 in raw and cooked beef (modification of AOAC Official Method 996.09): collaborative study.
AOAC Official Method 996.09, Visual Immunoprecipitate Assay (VIP) for Escherichia coli O157:H7, was modified to incorporate a new enrichment protocol using BioControl EHEC8 medium for testing raw and cooked beef. Foods were tested by VIP assay and the U.S. Department of Agriculture/Food Safety and Inspection Service (USDA/FSIS) enrichment procedure and the FDA Bacteriological Analytical Manual (BAM) isolation and confirmation techniques. A total of 15 collaborators participated. Raw and cooked ground beef were inoculated with E. coli O157:H7 at 2 different levels: a high level, where predominantly positive results were expected, and a low level where fractional recovery was anticipated. Collaborators tested 396 test portions and controls by both methods, for a total of 792 test portions. Of the 396 paired test portions, 75 were positive and 230 were negative by both the VIP and culture methods. Eleven test portions were presumptively positive by VIP and could not be confirmed culturally; 32 were negative by VIP, but confirmed positive by culture; and 65 were negative by the culture method, but confirmed positive by the VIP method. There was no statistical difference between results obtained with the VIP for EHEC 8 h method and the culture method except for cooked beef, where the VIP had significantly higher recovery for one inoculation level.
Feldsine
Philip T
PT
BioControl Systems, Inc., Bellevue, WA 98005, USA. ptf@biocontrolsys.com
Kerr
David E
DE
Leung
Stephanie C
SC
Lienau
Andrew H
AH
Moser
Ruth F
RF
Mui
Linda A
LA
D000818:Animals; D002417:Cattle; D003470:Culture Media; D019453:Escherichia coli O157 / Q000737:chemistry* / Q000302:isolation & purification; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007202:Indicators and Reagents; D008460:Meat / Q000382:microbiology*; D011233:Precipitin Tests; D015203:Reproducibility of Results
D003470:Culture Media; D007202:Indicators and Reagents