New HLA-B alleles identified and sequences extended in potential bone marrow donors: B*5804, B*4418, B*1558, and B*4805.
Nucleic acid-based methods for allele identification have revealed more than 470 polymorphic variants at the HLA-B locus. Screening of potential bone marrow donors with sequence specific primer polymerase chain reactions and sequence specific oligonucleotide probe hybridization assays revealed apparent variants within the B*58, *44, *15, and *48 allele groups. DNA sequencing of cloned DNA identified the new alleles B*5804, B*4418, and B*1558 within these groups and observed new sequence information for the previously reported allele B*4805. These findings further extend our knowledge of the substantial genetic variation present at the HLA-B locus within human populations.
Williams
T M
TM
Department of Pathology, University of New Mexico, Albuquerque 87131-5051, USA. twilliams@salud.unm.edu
Wu
J
J
Bassinger
S
S
Feldman
D
D
Birkos
S
S
Kearns
J
J
Kamoun
M
M
D000483:Alleles; D019943:Amino Acid Substitution / Q000235:genetics; D001483:Base Sequence; D044383:Black People / Q000235:genetics; D016026:Bone Marrow Transplantation; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D033581:Stem Cell Transplantation; D014019:Tissue Donors
D015235:HLA-B Antigens