title: Effect of loss of bite force on interleukin-1beta expression in rat periodontium.
abstract: OBJECTIVE
  To study the expression of interleukin-1beta(IL-1beta) in the fibroblasts and osteoblasts in the periodontal ligament cells(PDLC) of normal rats and rats with loss of bite force, to explain the molecular mechanism of periodontium remodeling.
  
  
  METHODS
  Rat models of lost bite force were established using Wistar rats. The changes in the histological morphology of the periodontium and the expression of IL-1beta in the PDLC and osteoblasts were observed in both normal rats and the rat models using HE staining and immunohistochemistry techniques.
  
  
  RESULTS
  In rat models of lost bite force, the periodontal ligament structure appeared to be disorganized and absorption occurred in the alveolar bone. The expression level of IL-1beta in the PDLC and osteoblasts was significantly enhanced in comparison with that in the normal rats, as demonstrated by immunohistochemical study.
  
  
  CONCLUSIONS
  Loss of bite force may induce structural disorganization of the periodontal ligament and absorption in the alveolar bone, and the expression level of IL-1beta in PDLC and osteoblasts show significant elevation, indicating that the changes in histological morphology is closely coordinated with the functional changes in the periodontium.
authors.affiliation: Department of Stomatology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001732:Bite Force; D003766:Dental Occlusion; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods; D007375:Interleukin-1 / Q000096:biosynthesis*; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000276:immunology; D010513:Periodontal Ligament / Q000166:cytology / Q000276:immunology; D010519:Periodontium / Q000166:cytology / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007375:Interleukin-1