Structural heterogeneity of the streptococcal C5a peptidase gene in Streptococcus pyogenes.
The 3' ends of the genes for the C-terminal region of C5a peptidase from 15 Streptococcus pyogenes isolates were analyzed by PCR. Amplicons were found to differ in size. DNA sequence analysis revealed that the differences between PCR fragment sizes accorded with the number of R repeats in the C5a peptidase gene.
Koroleva
Irina V
IV
Institute of Experimental Medicine, St. Petersburg, Russia.
Efstratiou
Androulla
A
Suvorov
Alexander N
AN
D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D010450:Endopeptidases / Q000737:chemistry* / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification
D018829:Adhesins, Bacterial; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010450:Endopeptidases; C053088:C5a peptidase