Pitfalls in cefepime titration from human plasma: plasma- and temperature-related drug degradation in vitro.
While developing a high-pressure liquid chromatography assay for cefepime in plasma, we observed significant drug degradation at 20 and 37 degrees C but not at 4 degrees C. This plasma-related degradation persisted after protein removal. This warrants caution regarding cefepime assays for pharmacokinetic and pharmacodynamic studies of cefepime in vitro and in vivo.
Bugnon
Denis
D
Division of Infectious Diseases, Department of Internal Medicine, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland.
Giannoni
Eric
E
Majcherczyk
Paul
P
Glauser
Michel P
MP
Moreillon
Philippe
P
D000077723:Cefepime; D002511:Cephalosporins / Q000097:blood* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D015203:Reproducibility of Results; D013696:Temperature; D014462:Ultrafiltration
D002511:Cephalosporins; D000077723:Cefepime