The protein-tyrosine phosphatase CD45 reaches the cell surface via golgi-dependent and -independent pathways.
CD45 is a receptor protein-tyrosine phosphatase essential for T cell development and lymphocyte activation. It is highly glycosylated, with multiple isoforms and glycoforms expressed on the cell surface depending on the cell type and stage of differentiation. Interestingly, we found two pools of newly synthesized CD45 expressed on plasma membrane, one of which arrived by 5 min after synthesis. The remaining pool of CD45 was fully glycosylated and began to arrive at the cell surface at approximately 15 min. The rapidly expressed population of CD45 possessed exclusively endoglycosidase H-sensitive N-linked carbohydrate. Additionally, this rapidly expressed pool of CD45 appeared on the cell surface in a brefeldin A (BFA)-insensitive manner, suggesting that it reached the cell surface independent of the Golgi complex. The remaining CD45 trafficked through the Golgi complex, and transport proceeded via a BFA-sensitive mechanism. These data suggest that CD45 is able to reach the cell surface via two distinct routes. The first is a conventional Golgi-dependent pathway that allows fully processed CD45 to be expressed. The second utilizes an ill defined mechanism that is independent of the Golgi, is BFA-resistant, and allows for the expression of CD45 with immature carbohydrate on the cell surface.
Baldwin
Troy A
TA
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada.
Ostergaard
Hanne L
HL
D015703:Antigens, CD / Q000302:isolation & purification / Q000378:metabolism; D019921:Biotinylation; D020126:Brefeldin A / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology*; D005347:Fibroblasts; D006056:Golgi Apparatus / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D017493:Leukocyte Common Antigens / Q000302:isolation & purification / Q000378:metabolism*; D016399:Lymphoma, T-Cell; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D021381:Protein Transport; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D011500:Protein Synthesis Inhibitors; D020126:Brefeldin A; D017493:Leukocyte Common Antigens