[Isolation of endo-1,4-beta-xylanase from Geotrichum candidum 3C with various ability of sorption on an insoluble substrate].
Culture liquid from Geotrichum candidum 3C was shown to contain three endoxylanase types: endoxylanase I that binds to cellulose, endoxylanase II that sorbs to insoluble xylan, and endoxylanase III that cannot sorb to dissoluble substrate. The catalytic and substrate-binding domains of endoxylanase II were isolated.
Rodionova
N A
NA
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071 Russia.
Dubovaia
N V
NV
Odintsova
T I
TI
Gracheva
I M
IM
Bezborodov
A M
AM
D004591:Electrophoresis, Polyacrylamide Gel; D043364:Endo-1,4-beta Xylanases; D005848:Geotrichum / Q000201:enzymology*; D012995:Solubility; D013379:Substrate Specificity; D014995:Xylosidases / Q000302:isolation & purification* / Q000378:metabolism
D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases