title: Mass spectrometric proteome analyses of synovial fluids and plasmas from patients suffering from rheumatoid arthritis and comparison to reactive arthritis or osteoarthritis.
abstract: Differential proteome analysis is used to study body fluids from patients suffering from rheumatoid arthritis (RA), reactive arthritis (reaA) or osteoarthritis (OA). Mass spectrometric structure characterization of gel-separated proteins provided a detailed view of the protein-processing events that lead to distinct protein species present in the respective body fluids. (i) Fibrin(ogen) beta-chain degradation products, presumably plasmin-derived, appeared solely in synovial fluids (SF) from both patient collectives, (ii) calgranulin B (MRP14) was exclusively identified in SF samples derived from 5 out of 6 patients suffering from RA. Calgranulin B was not observed in synovial fluids from OA patients, nor in plasmas from either patient group. In all cases where calgranulin B was detected, calgranulin C was identified as well. (iii) Serum amyloid A protein spots were determined in plasmas and synovial fluids from patients with RA, but not in patients with OA. In addition to disease-relevant differences, interindividual differences in haptoglobin patterns of the patients under investigation were observed. Hence, in-depth proteome analysis of body fluids has proven effective for identification of multiple molecular markers and determination of associated protein structure modifications, that are thought to play a role for specifically determining a defined pathological state of diseased joints.
authors.affiliation: Proteome Center Rostock, Medical Faculty, University of Rostock, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Sinz
authors.forename: Marcus
authors.initials: M
authors.lastname: Bantscheff
authors.forename: Stefan
authors.initials: S
authors.lastname: Mikkat
authors.forename: Bruno
authors.initials: B
authors.lastname: Ringel
authors.forename: Susanne
authors.initials: S
authors.lastname: Drynda
authors.forename: Jörn
authors.initials: J
authors.lastname: Kekow
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Thiesen
authors.forename: Michael O
authors.initials: MO
authors.lastname: Glocker
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D016918:Arthritis, Reactive / Q000097:blood / Q000378:metabolism*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D008970:Molecular Weight; D010003:Osteoarthritis / Q000097:blood / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010949:Plasma / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013582:Synovial Fluid / Q000737:chemistry*
chemical_list: D010446:Peptide Fragments; D020543:Proteome; D005340:Fibrinogen