Actions of gluco-oligosaccharide against Staphylococcus aureus.
Heavy colonization of atopic dermatitis (AD) with Staphylococcus aureus is well documented. The purpose of the present study is to examine the actions of gluco-oligosaccharide (G-OS) against S. aureus for the control of AD skin lesions infected with S. aureus. The colony counts of S. aureus cells in 0.5% sodium chloride solution supplemented with 5% G-OS (pH 4.8) were about 10-fold lower than those in 0.5% sodium chloride solution (pH 6.6; control) after incubation for 24 hours. The colony counts of S. aureus cells attached on the coverslips (pre-treatment with 1% and 5% G-OS/PBS and following treatment with plasma) were about 10-fold lower than those on the coverslips (pre-treatment with PBS and following treatment with plasma; control) in PBS after incubation for 24 hours. The materials (sugars, probably glycocalyx) that stained positively for fluorescein-isothiocyanate (FITC) -concanavalin A and were consistent with the presence of S. aureus cells were reduced when S. aureus cells attached to the coverslips treated with 5% GC-OS. In conclusion, C-OS is a promising agent that can be applied topically in a cream to clear adherent S. aureus cells from skin lesions of AD in order to prevent its exacerbation. Further, 5% C-OS can inhibit glycocalyx production by S. aureus cells and consequently have some suppressive effect on the colonization of S. aureus on the horny cells of AD lesions.
Akiyama
Hisanori
H
Department of Dermatology, Okayama University Graduate School of Medicine and Dentistry, Japan.
Oono
Takashi
T
Huh
Wook Kang
WK
Yamasaki
Osamu
O
Akagi
Yukie
Y
Uemura
Hideto
H
Yamada
Tetsumasa
T
Iwatsuki
Keiji
K
D015169:Colony Count, Microbial; D003470:Culture Media; D003876:Dermatitis, Atopic / Q000188:drug therapy / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D009844:Oligosaccharides / Q000494:pharmacology*; D012016:Reference Values; D012680:Sensitivity and Specificity; D013207:Staphylococcal Skin Infections / Q000175:diagnosis / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification
D003470:Culture Media; D009844:Oligosaccharides