Functional proteomics to study protection of the ischaemic myocardium.
Mechanisms to reduce the deleterious effects of myocardial ischaemia are of particular clinical importance and have been the focus of intense research for a number of years. Among novel approaches to studying the ischaemic heart, proteomics, or the analysis of all cellular proteins, presents as a powerful method to deconstruct the mechanisms of disease and protection. Specifically, the field of functional proteomics is an emerging application of proteomics that melds aspects of classical proteomics, biochemistry, molecular biology and physiology into an approach that facilitates an understanding of how proteins and protein interactions engender phenotype. This review highlights different types of proteomic applications and provides a prospectus for functional proteomics as a robust vehicle driving drug discovery and design.
Vondriska
Thomas M
TM
Department of Physiology, Division of Cardiology, University of California, Los Angeles, CA 90095, USA.
Ping
Peipei
P
D000818:Animals; D015415:Biomarkers; D002316:Cardiotonic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002318:Cardiovascular Diseases / Q000453:epidemiology; D015195:Drug Design; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000517:prevention & control; D010449:Peptide Mapping; D010641:Phenotype; D011493:Protein Kinase C / Q000502:physiology; D051744:Protein Kinase C-epsilon; D040901:Proteomics; D015398:Signal Transduction
D015415:Biomarkers; D002316:Cardiotonic Agents; C497256:Prkce protein, mouse; C497255:PRKCE protein, human; D011493:Protein Kinase C; D051744:Protein Kinase C-epsilon