title: Terminal glycosylation in cystic fibrosis (CF): a review emphasizing the airway epithelial cell.
abstract: Altered terminal glycosylation, with increased fucosylation and decreased sialylation is a hallmark of the cystic fibrosis (CF) glycosylation phenotype. Oligosaccharides purified from the surface membrane glycoconjugates of CF airway epithelial cells have the Lewis x, selectin ligand in terminal positions. This review is focused on the investigations of the glycoconjugates of the CF airway epithelial cell surface. Two of the major bacterial pathogens in CF, Pseudomonas aeruginosa and Haemophilus influenzae, have binding proteins which recognize fucose in alpha-1,3 linkage and asialoglycoconjugates. Therefore, consideration has been given to the possibility that the altered terminal glycosylation of airway epithelial glycoproteins in CF contributes to both the chronic infection and the robust, but ineffective, inflammatory response in the CF lung. Since the glycosylation phenotype of CF airway epithelial cells have been modulated by the expression of wtCFTR, the hypotheses which have been proposed to relate altered function of CFTR to the regulation of the glycosyltransferases are discussed. Understanding the effects of mutant CFTR on glycosylation may provide further insight into the regulation of glycoconjugate processing as well as new approaches to the therapy of CF.
authors.affiliation: The Cystic Fibrosis Center and Department of Pediatrics, University of Pennsylvania School of Medicine, The Children's Hospital of Philadelphia, 3516 Civic Center Boulevard, Philadelphia, PA 19104, USA.
authors.forename: A D
authors.initials: AD
authors.lastname: Rhim
authors.forename: L
authors.initials: L
authors.lastname: Stoykova
authors.forename: M C
authors.initials: MC
authors.lastname: Glick
authors.forename: T F
authors.initials: TF
authors.lastname: Scanlin
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D003550:Cystic Fibrosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005643:Fucose / Q000378:metabolism; D005647:Fucosyltransferases / Q000378:metabolism; D006031:Glycosylation; D006193:Haemophilus influenzae / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D012137:Respiratory System / Q000378:metabolism* / Q000473:pathology; D012799:Sialyltransferases / Q000378:metabolism; D021601:trans-Golgi Network / Q000378:metabolism
chemical_list: C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005643:Fucose; D005647:Fucosyltransferases; D012799:Sialyltransferases