title: Evaluation of the murine immune response to Leishmania meta 1 antigen delivered as recombinant protein or DNA vaccine.
abstract: The meta 1 gene of Leishmania is conserved across the genus and encodes a protein upregulated in metacyclic promastigotes. Meta 1 constitutive overexpressing mutants show increased virulence to mice. In this paper, both meta 1 recombinant protein and plasmids bearing the meta 1 gene were tested for their antigenicity and potential for inducing protective immunity in mice. Vaccination with the recombinant protein induced a predominant Th2-type of response and did not result in protection upon challenge with live parasites. Surprisingly, the expected reversal to a CD4(+) Th1-type of response upon genetic immunisation by the intramuscular route was not observed. Instead, vaccination with either the meta 1 gene alone or in fusion with the monocyte chemotactic protein (MCP)-3 cDNA induced a Th2-type of response that correlated with lack of protection against infection.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av Prof Lineu Prestes, 1374, São Paulo, CEP 05508-900 Brazil.
authors.forename: Carlos Henrique Cardoso
authors.initials: CH
authors.lastname: Serezani
authors.forename: Amanda Richards
authors.initials: AR
authors.lastname: Franco
authors.forename: Mariana
authors.initials: M
authors.lastname: Wajc
authors.forename: Jenicer K
authors.initials: JK
authors.lastname: Umada Yokoyama-Yasunaka
authors.forename: Gerhard
authors.initials: G
authors.lastname: Wunderlich
authors.forename: Monamaris Marques
authors.initials: MM
authors.lastname: Borges
authors.forename: Silvia Reni Bortolin
authors.initials: SR
authors.lastname: Uliana
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D019556:COS Cells / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D054410:Chemokine CCL7; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D018320:Leishmania major / Q000276:immunology*; D007894:Leishmania mexicana / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000517:prevention & control; D016774:Leishmaniasis, Diffuse Cutaneous / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D018945:Monocyte Chemoattractant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D015800:Protozoan Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D016052:Protozoan Vaccines / Q000008:administration & dosage* / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D012995:Solubility; D016176:T-Lymphocyte Subsets / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology / Q000627:therapeutic use; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; C515451:Ccl7 protein, mouse; D054410:Chemokine CCL7; D016207:Cytokines; C100528:Meta1 protein, Leishmania major; D018945:Monocyte Chemoattractant Proteins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic