title: Effects of the osmolyte trimethylamine-N-oxide on conformation, self-association, and two-dimensional crystallization of myelin basic protein.
abstract: The osmolyte trimethylamine-N-oxide (TMAO) is a naturally in vivo occurring "chemical chaperone" that has been shown to stabilise the folding of numerous proteins. Myelin basic protein (MBP) is a molecule that has not yet been suitably crystallized either in three dimensions for X-ray crystallography or in two dimensions for electron crystallography. Here, we describe lipid monolayer crystallization experiments of two species of recombinant murine MBP in the presence of TMAO. One protein was unmodified, whereas the other contained six Arg/Lys-->Gln substitutions to mimic the effects of deimination (i.e., the enzymatic modification of Arg to citrulline), which reduces the net positive charge. Planar arrays of both proteins were formed on binary lipid monolayers containing a nickel-chelating lipid and a phosphoinositide. In the presence of TMAO, the diffraction spots of these arrays became sharper and more distinct than in its absence, indicating some improvement of crystallinity. The osmolyte also induced the formation of epitaxial growth of protein arrays, especially with the mutant protein. However, none of these assemblies was sufficiently ordered to extract high-resolution structural information. Circular dichroic spectroscopy showed that MBP gained no increase in ordered secondary structure in the presence of TMAO in bulk solution, whereas it did in the presence of lipids. Dynamic light-scattering experiments confirmed that the MBP preparations were monomodal under the optimal crystallization conditions determined by electron microscopy trials. The salt and osmolyte concentrations used were shown to result in a largely unassociated population of MBP. The amino acid composition of MBP overwhelmingly favours a disordered state, and a neural-network-based scheme predicted large segments that would be unlikely to adopt a regular conformation. Thus, this protein has an inherently disordered nature, which mitigates strongly against its crystallization for high-resolution structure determination.
authors.affiliation: Department of Molecular Biology and Genetics, University of Guelph, 50 Stone Road East, Guelph, Ont., Canada N1G 2W1.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Hill
authors.forename: Ian R
authors.initials: IR
authors.lastname: Bates
authors.forename: Gisele F
authors.initials: GF
authors.lastname: White
authors.forename: F Ross
authors.initials: FR
authors.lastname: Hallett
authors.forename: G
authors.initials: G
authors.lastname: Harauz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000737:chemistry; D002942:Circular Dichroism; D003460:Crystallization; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005973:Glutamine / Q000737:chemistry; D008027:Light; D050356:Lipid Metabolism; D008239:Lysine / Q000737:chemistry; D008744:Methylamines / Q000494:pharmacology*; D051379:Mice; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009154:Mutation; D004676:Myelin Basic Protein / Q000737:chemistry* / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology*; D011485:Protein Binding; D011487:Protein Conformation; D020033:Protein Isoforms; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012542:Scattering, Radiation; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D008744:Methylamines; D004676:Myelin Basic Protein; D016877:Oxidants; D020033:Protein Isoforms; D011994:Recombinant Proteins; D005973:Glutamine; D012965:Sodium Chloride; D001120:Arginine; C005855:trimethyloxamine; D008239:Lysine