title: Citrate carrier activity and cardiolipin level in eel (Anguilla anguilla) liver mitochondria.
abstract: The activity of the tricarboxylate (citrate) carrier has been assayed in intact liver mitochondria from yellow eel (Anguilla anguilla) and compared to that from rat. The eel-citrate carrier specific activity was approximately 1.7-fold higher than that assayed in rat-liver mitochondria. The content of the main mitochondrial phospholipids, phosphatidylethanolamine and phosphatidylcholine, did not show a significant difference between the two species, while in eel a higher cardiolipin level was observed. Fatty acid composition of eel-liver mitochondrial phospholipids was characterised by a large amount of unsaturated fatty acids, dominated by octadecaenoic acid (C(18:1) (n-9)) and docosahexaenoic acid (C(22:6) (n-3)). The cardiolipin fatty acid pattern of eel-liver mitochondria showed, with respect to the rat, a higher C(20:5) (n-3) and C(22:6) (n-3) content and a lower amount of C(18:2) (n-6) and C(20:4) (n-6). A noticeable activity of lipogenic enzymes was also detected in eel liver cytosol. The results of this study suggest that the remarkable activity of the citrate carrier in eel-liver mitochondria can most likely be ascribed to a considerable cardiolipin level. A covariance of citrate carrier and lipogenic enzyme activities was observed.
authors.affiliation: Laboratory of Biochemistry, Department of Biological and Environmental Sciences and Technologies, University of Lecce, Via per Monteroni, 73100 Lecce, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Giudetti
authors.forename: L
authors.initials: L
authors.lastname: Siculella
authors.forename: G V
authors.initials: GV
authors.lastname: Gnoni
mesh_terms: D000818:Animals; D002308:Cardiolipins / Q000032:analysis*; D002352:Carrier Proteins / Q000378:metabolism*; D019343:Citric Acid / Q000378:metabolism; D004524:Eels / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D007425:Intracellular Membranes / Q000737:chemistry; D008293:Malates / Q000378:metabolism; D008930:Mitochondria, Liver / Q000737:chemistry* / Q000648:ultrastructure; D010743:Phospholipids / Q000032:analysis; D051381:Rats
chemical_list: D002308:Cardiolipins; D002352:Carrier Proteins; D005227:Fatty Acids; D008293:Malates; D010743:Phospholipids; C020723:citrate-binding transport protein; D019343:Citric Acid; C030298:malic acid