title: Yeast Mon1p/Aut12p functions in vacuolar fusion of autophagosomes and cvt-vesicles.
abstract: Here we identify Mon1p as being essential for the cvt-pathway and autophagy. Thus, mon1Delta cells are impaired in proaminopeptidase I maturation and homozygous diploid mon1Delta cells do not sporulate. Quantitative autophagy measurements suggest a complete autophagy block. The autophagosomal marker protein GFP-Aut7p accumulates in mon1Delta cells at punctate structures outside the vacuole. Furthermore, proaminopeptidase I accumulates in mon1Delta cells in a proteinase-protected form. Our data demonstrate that mon1Delta cells are defective in the fusion of cvt-vesicles and autophagosomes with the vacuole. Consistent with this, GFP-Mon1p localizes to the cytosol and to punctate structures within the cytosol.
authors.affiliation: Institute of Biochemistry, University of Stuttgart, Pfaffenwaldring 55, Germany.
authors.forename: Khuyen
authors.initials: K
authors.lastname: Meiling-Wesse
authors.forename: Henning
authors.initials: H
authors.lastname: Barth
authors.forename: Christiane
authors.initials: C
authors.lastname: Voss
authors.forename: Gunilla
authors.initials: G
authors.lastname: Barmark
authors.forename: Eva
authors.initials: E
authors.lastname: Murén
authors.forename: Hans
authors.initials: H
authors.lastname: Ronne
authors.forename: Michael
authors.initials: M
authors.lastname: Thumm
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism; D001343:Autophagy; D001483:Base Sequence; D017931:DNA Primers; D005656:Fungal Proteins / Q000378:metabolism*; D010588:Phagosomes / Q000201:enzymology / Q000502:physiology*; D011498:Protein Precursors / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D014617:Vacuoles / Q000201:enzymology / Q000502:physiology*
chemical_list: D017931:DNA Primers; D005656:Fungal Proteins; D011498:Protein Precursors; D000626:Aminopeptidases; C450422:proaminopeptidase I