title: [Differences in gene expression between Taxus chinensis cells during Taxol-synthesis phase and those during non-Taxol-synthesis phase].
abstract: In plant, evocation of secondary metabolism is associated with complex biochemical and molecular events that are regulated by developmental and environmental factors. In order to get more information about Taxol biosynthesis, comparison of mRNA populations from Taxus chinensis cells during Taxol-synthesis phase and those during non-Taxol-synthesis phase were performed by mRNA differential display. The results suggested that genes specifically expressed in the Taxol-synthesis phase might be involved in Taxol biosynthesis.
authors.affiliation: School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. hugbonline@263.net
authors.forename: Guo-Bin
authors.initials: GB
authors.lastname: Hu
authors.forename: Xing-Guo
authors.initials: XG
authors.lastname: Mei
authors.forename: Wei
authors.initials: W
authors.lastname: Gong
authors.forename: Tie
authors.initials: T
authors.lastname: Ke
mesh_terms: D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017239:Paclitaxel / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020946:Taxus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D017239:Paclitaxel