title: [Purification and renaturation of recombinant human interleukin-2-pseudomonas exotoxin (IL2-PE66(4GLU)) fusion protein].
abstract: OBJECTIVE
  To evaluate the effect of a novel approach for purification and renaturation of recombinant human interleukin-2-pseudomonas exotoxin (IL2-PE66(4Glu)) fusion protein.
  
  
  METHODS
  A novel purification method established in our laboratory was adopted for the purification of the inclusion body, and after renaturation, recombinant human IL2-PE66(4Glu) fusion protein was purified by DEAE-Sepharose FF ion-exchange chromatography.
  
  
  RESULTS
  The purity of the fusion protein that retain its biological activity was as high as 95%, and a recovery rate over 80% of the refolded IL2-PE66(4Glu) fusion protein was achieved.
  
  
  CONCLUSIONS
  The purification and refolding method for inclusion body adopted in this study is simple and practical, which lays the foundation for a large-scale production of the fusion protein.
authors.affiliation: Institute of Molecular Immunology, First Military Medical University, Guangzhou 510515, China. hzm@fimmu.edu.cn
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Hu
authors.forename: Xing-Mei
authors.initials: XM
authors.lastname: Linlai
authors.forename: Ming-Qian
authors.initials: MQ
authors.lastname: Zhou
authors.forename: Ze-Hong
authors.initials: ZH
authors.lastname: Chen
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Wang
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005098:Exotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D020673:Protein Renaturation; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D005098:Exotoxins; D007376:Interleukin-2; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C065698:interleukin 2-PE66(4Glu) protein, recombinant