Cytochrome P450 1B1 mRNA levels in peripheral blood cells and exposure to polycyclic aromatic hydrocarbons in Chinese coke oven workers.
Cytochrome P450 1B1 (CYP1B1) is induced through the Ah receptor and is involved in the activation of polycyclic aromatic hydrocarbons (PAHs). To determine the validity of a quantitative analysis of CYP1B1 mRNA in peripheral human blood cells for the estimation of PAH exposure, a real-time quantitative polymerase chain reaction method was used to measure the relative levels of CYP1B1 mRNA in 37 Chinese coke oven workers and 13 control workers. A large inter-individual difference in the levels was observed. The average level of the CYP1B1 mRNA in workers at the top work site, where the PAH exposure level from the coke ovens was highest, was significantly higher than in workers at the middle site (P<0.01) or the controls (P=0.02). A non-significant positive correlation was found between the CYP1B1 mRNA levels and urinary 1-hydroxypyrene (R=0.22, P=0.13), and a significant correlation between these mRNA levels and urinary cotinine (R=0.33, P=0.02). It was interesting that a significant positive correlation between CYP1B1 mRNA and 1-hydroxypyrene was observed in subjects with the Leu/Leu type of CYP1B1 Leu432Val polymorphism (R=0.33, P=0.02, n=38) and a non-significant correlation in subjects with the Leu/Val and Val/Val types (R=-0.36, P=0.25, n=12), although the number of subjects in this strata analysis was small. Our preliminary study suggests that PAH exposure in coke ovens and smoking maybe associated with CYP1B1 mRNA levels in peripheral blood cells although mRNA is generally unstable and could be expressed following exposure to other agents.
Hanaoka
Tomoyuki
T
Epidemiology and Biostatistics Division, National Cancer Center Research Institute East, Kashiwa-shi, Chiba, Japan. thanaoka@east.ncc.go.jp
Yamano
Yuko
Y
Pan
Guowei
G
Hara
Kunio
K
Ichiba
Masayoshi
M
Zhang
Jiusong
J
Zhang
Shujuan
S
Liu
Tiefu
T
Li
Landi
L
Takahashi
Ken
K
Kagawa
Jun
J
Tsugane
Shoichiro
S
D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis*; D015415:Biomarkers / Q000032:analysis; D001773:Blood Cells; D002681:China; D003077:Coke / Q000009:adverse effects*; D065633:Cytochrome P-450 CYP1B1; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects*; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000032:analysis; D012680:Sensitivity and Specificity; D012907:Smoking / Q000009:adverse effects
D015415:Biomarkers; D003077:Coke; D011084:Polycyclic Aromatic Hydrocarbons; D012333:RNA, Messenger; D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1