Microbial community in acidic hydrothermal waters of volcanically active White Island, New Zealand.
We report the first description of the microbial community in a stream of acidic hydrothermal waters on volcanically active White Island, New Zealand, using both molecular and microbiological methods. alpha- and beta-Proteobacteria, green-sulfur bacteria, and uncultured Firmicutes were identified from the community DNA-based 16s rRNA gene library. The same bacterial groups and the Rhodophyte Cyanidium caldarium were represented in enrichment cultures. C. caldarium, two Firmicutes and an acidophilic alpha-Proteobacterium, Acidiphilium cryptum, were brought into pure culture. Bacteria cultured from the stream grow at pH > or =2, and the Cyanidium grows at pH 0.2.
Donachie
Stuart P
SP
Department of Microbiology, University of Hawai'i, Snyder Hall, 2538 The Mall, Honolulu, HI 96822, USA.
Christenson
Bruce W
BW
Kunkel
Dennis D
DD
Malahoff
Alexander
A
Alam
Maqsudul
M
D020561:Alphaproteobacteria / Q000302:isolation & purification; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D020562:Betaproteobacteria / Q000302:isolation & purification; D019414:Chlorobi / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D019143:Evolution, Molecular; D015723:Gene Library; D006094:Gram-Positive Bacteria / Q000302:isolation & purification; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D009520:New Zealand; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D018707:Volcanic Eruptions; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S