Application of solid-phase microextraction to the quantitative analysis of 1,8-cineole in blood and expired air in a Eucalyptus herbivore, the brushtail possum (Trichosurus vulpecula).
We have developed two solid-phase microextraction (SPME) methods, coupled with gas chromatography, for quantitatively analysing the major Eucalyptus leaf terpene, 1,8-cineole, in both expired air and blood from the common brushtail possum (Trichosurus vulpecula). In-line SPME sampling (5 min at 20 degrees C room temperature) of excurrent air from an expiratory chamber containing a possum dosed orally with 1,8-cineole (50 mg/kg) allowed real-time semi-quantitative measurements reflecting 1,8-cineole blood concentrations. Headspace SPME using 50 microl whole blood collected from possums dosed orally with 1,8-cineole (30 mg/kg) resulted in excellent sensitivity (quantitation limit 1 ng/ml) and reproducibility. Blood concentrations ranged between 1 and 1380 ng/ml. Calibration curves were prepared for two concentration ranges (0.05-10 and 10-400 ng/50 microl) for the analysis of blood concentrations. Both calibration curves were linear (r(2)=0.999 and 0.994, respectively) and the equations for the two concentration ranges were consistent.
Boyle
Rebecca R
RR
Tasmanian School of Pharmacy, University of Tasmania, GPO Box 252-26, Hobart 7001, Tasmania, Australia. rebecca.boyle@utas.edu.au
McLean
Stuart
S
Brandon
Sue
S
Pass
Georgia J
GJ
Davies
Noel W
NW
D000388:Air / Q000032:analysis*; D000818:Animals; D001944:Breath Tests; D002138:Calibration; D003511:Cyclohexanols / Q000032:analysis / Q000097:blood*; D000077591:Eucalyptol; D005052:Eucalyptus; D039821:Monoterpenes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013729:Terpenes / Q000032:analysis / Q000097:blood*
D003511:Cyclohexanols; D039821:Monoterpenes; D013729:Terpenes; D000077591:Eucalyptol