title: [Effects of androgen and estrogen on the expressions of basic fibroblast growth factor, transforming growth factor and smoothelin].
abstract: OBJECTIVE
  To study effects of androgen and estrogen on the expressions of basic fibroblast growth factor (bFGF) and transforming growth factor beta2 (TGFbeta2) in cultured human prostatic stromal cells.
  
  
  METHODS
  Human prostatic stromal cells obtained from 11 patients with benign prostatic hypertrophy (BPH) were cultured and stimulated with dihydrotestosterone (DHT) and estradiol (E2). The expressions of bFGF and TGFbeta2 mRNA along with smoothelin mRNA were observed with reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  DHT significantly upregulated bFGF expression, and E2 enhanced TGFbeta2 and smoothelin expressions. A positive correlation between expressions of TGFbeta2 and smoothelin was observed.
  
  
  CONCLUSIONS
  DHT can induce bFGF expression and E2 promotes TGFbeta2 expression, and the transformation toward smooth muscle cells induced by E(2) may involve the action of TGFbeta2.
authors.affiliation: Department of Urology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Shao-Bin
authors.initials: SB
authors.lastname: Zheng
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D003598:Cytoskeletal Proteins / Q000096:biosynthesis; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis; D011467:Prostate / Q000166:cytology; D017154:Stromal Cells / Q000187:drug effects* / Q000378:metabolism; D015291:Transforming Growth Factors / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D003598:Cytoskeletal Proteins; D009124:Muscle Proteins; C100762:SMTN protein, human; D013196:Dihydrotestosterone; D016222:Fibroblast Growth Factor 2; D004958:Estradiol; D015291:Transforming Growth Factors