A repeat biopsy study in spontaneous crescentic glomerulonephritis mice.
BACKGROUND
Spontaneous crescentic glomerulonephritis (SCG)/Kj mice are a candidate for suitable animal model of human pauci-immune crescentic glomerulonephritis (GN). In the present study, we used renal biopsy technique and analyzed time sequence correlations among crescent formation and glomerular neutrophil infiltration in SCG/Kj mice.


METHODS
We observed the progress of GN in SCG/Kj mice according to the urinary abnormalities, and performed the serial renal biopsies. The kinetics of histopathology and glomerular neutrophil influx corresponding disease stage were examined by enzyme-histochemistry and immunohistochemistry.


RESULTS
We divided natural course of GN into three periods in view of urinalysis: a proteinuria-negative/hematuria-negative (P- H-) period, followed by a proteinuria-positive/hematuria-negative (P+ H-) period, and finally a proteinuria-positive/hematuria-positive (P+ H+) period. Endocapillary proliferation phase existed in P+ H- period, whereas crescent formation occurred and extended in P+ H+ period. In P+ H- period, prominent glomerular neutrophil infiltration was observed, while these numbers decreased with the progression of crescent formation.


CONCLUSIONS
These observations suggest that there is a good correlation between urinalysis and histopathological events of SCG/Kj mice, and that endocapillary proliferation, which contains neutrophil infiltration, may contribute to the subsequent crescent formation in these mice.
Miyazawa
Shinobu
S
Department of Clinical Pharmacology, Research Institute, International Medical Center of Japan, Tokyo. smiyazawa@ri.imcj.go.jp
Saiga
Kan
K
Nemoto
Kyuichi
K
Mae
Takako
T
Hotta
Osamu
O
D000818:Animals; D001706:Biopsy; D004195:Disease Models, Animal; D005260:Female; D005919:Glomerular Filtration Rate / Q000502:physiology*; D005921:Glomerulonephritis / Q000473:pathology* / Q000503:physiopathology* / Q000652:urine; D006417:Hematuria / Q000473:pathology* / Q000503:physiopathology* / Q000652:urine; D007668:Kidney / Q000473:pathology / Q000503:physiopathology; D007678:Kidney Glomerulus / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000473:pathology / Q000502:physiology; D011507:Proteinuria / Q000473:pathology* / Q000503:physiopathology* / Q000652:urine; D012086:Reoperation; D013997:Time Factors