Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2.
We examined cytoplasmic trafficking and nuclear translocation of adeno-associated virus type 2 (AAV) by using Alexa Fluor 488-conjugated wild-type AAV, A20 monoclonal antibody immunocytochemistry, and subcellular fractionation techniques followed by DNA hybridization. Our results indicated that in the absence of adenovirus (Ad), AAV enters the cell rapidly and escapes from early endosomes with a t(1/2) of about 10 min postinfection. Cytoplasmically distributed AAV accumulated around the nucleus and persisted perinuclearly for 16 to 24 h. Viral uncoating occurred before or during nuclear entry beginning about 12 h postinfection, when viral protein and DNA were readily detected in the nucleus. Few, if any, intact AAV capsids were found in the nucleus. In the presence of Ad, however, cytoplasmic AAV quickly translocated into the nucleus as intact particles as early as 40 min after coinfection, and this facilitated nuclear translocation of AAV was not blocked by the nuclear pore complex inhibitor thapsigargan. The rapid nuclear translocation of intact AAV capsids in the presence of Ad suggested that one or more Ad capsid proteins might be altering trafficking. Indeed, coinfection with empty Ad capsids also resulted in the appearance of AAV DNA in nuclei within 40 min. Escape from early endosomes did not seem to be affected by Ad coinfection.
Xiao
Wu
W
Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, Florida 32610, USA.
Warrington
Kenneth H
KH
Hearing
Patrick
P
Hughes
Jeffrey
J
Muzyczka
Nicholas
N
D000260:Adenoviruses, Human / Q000472:pathogenicity* / Q000502:physiology; D002467:Cell Nucleus / Q000821:virology*; D003593:Cytoplasm / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D000229:Dependovirus / Q000472:pathogenicity* / Q000502:physiology; D005456:Fluorescent Dyes / Q000378:metabolism; D006367:HeLa Cells; D006378:Helper Viruses / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D022022:Nuclear Pore / Q000187:drug effects; D019284:Thapsigargin / Q000494:pharmacology
D004279:DNA, Viral; D005456:Fluorescent Dyes; D019284:Thapsigargin