title: Methodology of platelet membrane glycoproteins detection in whole blood by flow cytometry.
abstract: OBJECTIVE
  To establish a method for platelet membrane glycoproteins (PMGP) detection in the whole blood by flow cytometry (FCM) and to evaluate the methodological factors involved.
  
  
  METHODS
  Expressions of PMGP GP I b-I X, GP II b-III a and P-selectin were detected by FCM under different experimental conditions to evaluate the factors affecting their expressions.
  
  
  RESULTS
  FCM rapidly and accurately detected PMGP expressions with distinct specificity and sufficient sensitivity, and reduced the chances for artificial activation of the platelet during the experiment.
  
  
  CONCLUSIONS
  FCM provides a new method for PMGP function studies.
authors.affiliation: Central Laboratory, First Military Medical University, Guangzhou 510515, China.
authors.forename: Lian-Bo
authors.initials: LB
authors.lastname: Chen
authors.forename: Wei-Ren
authors.initials: WR
authors.lastname: Dong
authors.forename: Fang
authors.initials: F
authors.lastname: Dong
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006490:Hemostatics / Q000494:pharmacology; D006801:Humans; D008297:Male; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis*; D013917:Thrombin / Q000494:pharmacology
chemical_list: D006490:Hemostatics; D010980:Platelet Membrane Glycoproteins; D013917:Thrombin