title: Pitfalls in cefepime titration from human plasma: plasma- and temperature-related drug degradation in vitro.
abstract: While developing a high-pressure liquid chromatography assay for cefepime in plasma, we observed significant drug degradation at 20 and 37 degrees C but not at 4 degrees C. This plasma-related degradation persisted after protein removal. This warrants caution regarding cefepime assays for pharmacokinetic and pharmacodynamic studies of cefepime in vitro and in vivo.
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland.
authors.forename: Denis
authors.initials: D
authors.lastname: Bugnon
authors.forename: Eric
authors.initials: E
authors.lastname: Giannoni
authors.forename: Paul
authors.initials: P
authors.lastname: Majcherczyk
authors.forename: Michel P
authors.initials: MP
authors.lastname: Glauser
authors.forename: Philippe
authors.initials: P
authors.lastname: Moreillon
mesh_terms: D000077723:Cefepime; D002511:Cephalosporins / Q000097:blood* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D015203:Reproducibility of Results; D013696:Temperature; D014462:Ultrafiltration
chemical_list: D002511:Cephalosporins; D000077723:Cefepime