Effect of conditioned media from decidual cell culture on the expression of genes regulating the invasion of trophoblastic cells.
OBJECTIVE
To investigate the effect of the conditioned media from decidual cell cultures (DCM) on the expression of genes regulating the invasion of trophoblastic cells.


METHODS
In vitro culture of decidual cells obtained from healthy women of both early (within the first trimester) and full-term pregnancy respectively was performed to prepare conditioned media of decidual cells (DCM). Trophoblastic cells were also obtained in these subjects and treated with DCM for 24 h in in vitro culture, to observe the effect of DCM, with the help of semi-quantitative reverse transcriptase-PCR, on the expression of genes in these cells that regulate their invasion.


RESULTS
Expression of matrix metallsoproteinase (MMP)-2, MMP-9, tissue inhibitor of metalloproteinases (TIMP)-1 and urokinase-type plasminogen activator (u-PA), other than that of TIMP-2 and plasminogen activator inhibitor type (PAI)-1, was observed in normal trophoblastic cells in in vitro culture. DCM derived from wemon of early and full-term pregnancy down-regulated the expression of MMP-2, MMP-9, u-PA while up-regulated the expression of TIMP-1, PAI-1.


CONCLUSIONS
DCM may exhibit its anti-invasive activity by regulating the expression of genes involved in the regulation of trophoblastic cell invasion, such as MMP-2, MMP-9, TIMP-1, u-PA and PAI-1.
Zhang
Xi-Qian
XQ
Department of Obstetrics and Gynecology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Pang
Zhan-Jun
ZJ
Chen
Shi-Ling
SL
Xing
Fu-Qi
FQ
D017077:Culture Media, Conditioned; D010064:Embryo Implantation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness; D014327:Trophoblasts / Q000473:pathology* / Q000502:physiology
D017077:Culture Media, Conditioned