title: Radiographic evaluation of the effect of endotoxin (LPS) plus calcium hydroxide on apical and periapical tissues of dogs.
abstract: The aim of this study was the radiographic evaluation of the apical and periapical region of dog teeth submitted to intracanal bacterial endotoxin (lipopolysaccharide, LPS), associated or not with calcium hydroxide. After removal of the pulp, 60 premolars were divided into four groups and were filled with bacterial endotoxin (group 1), bacterial endotoxin plus calcium hydroxide (group 2), saline solution (group 3), or periapical lesions were induced with no treatment (group 4), for a period of 30 days. Similar periapical lesions were observed in groups 1 and 4. The lamina dura was intact in groups 2 and 3. Bacterial endotoxin (LPS) caused radiographically visible periapical lesions, but when associated with calcium hydroxide, this endotoxin was detoxified.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Brazil.
authors.forename: Paulo
authors.initials: P
authors.lastname: Nelson-Filho
authors.forename: Mario Roberto
authors.initials: MR
authors.lastname: Leonardo
authors.forename: Léa Assed Bezerra
authors.initials: LA
authors.lastname: Silva
authors.forename: Sada
authors.initials: S
authors.lastname: Assed
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000000981:diagnostic imaging; D001862:Bone Resorption / Q000000981:diagnostic imaging / Q000382:microbiology; D002126:Calcium Hydroxide / Q000627:therapeutic use*; D004285:Dogs; D004731:Endotoxins / Q000009:adverse effects* / Q000037:antagonists & inhibitors; D004926:Escherichia coli; D008070:Lipopolysaccharides / Q000009:adverse effects* / Q000037:antagonists & inhibitors; D010483:Periapical Diseases / Q000000981:diagnostic imaging / Q000382:microbiology; D010486:Periapical Tissue / Q000000981:diagnostic imaging* / Q000382:microbiology; D010513:Periodontal Ligament / Q000000981:diagnostic imaging; D011859:Radiography; D012387:Root Canal Filling Materials / Q000627:therapeutic use*; D012391:Root Resorption / Q000000981:diagnostic imaging / Q000382:microbiology; D018709:Statistics, Nonparametric; D019227:Tooth Apex / Q000000981:diagnostic imaging* / Q000382:microbiology
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides; D012387:Root Canal Filling Materials; D002126:Calcium Hydroxide