title: Oxygen-independent poly(dimethylsiloxane)-based carbon-paste glucose biosensors.
abstract: Several silicone oils have been assessed and compared as an internal source of oxygen in connection to their use as binders for carbon-paste glucose biosensors. All four poly(dimethylsiloxane) (PDMS) oils tested a dramatic increase in the oxygen capacity of carbon-paste enzyme electrodes to allow convenient biosensing under severe oxygen-deficit conditions. The resulting oxygen independence is better than that exerted by perfluorocarbon binders or that displayed by mediator-based bioelectrodes. The resistance to oxygen effects is indicated from the identical response (observed in the presence and absence of oxygen) up to 2 x 10(-2) M glucose and the slight (12%) sensitivity loss at 4 x 10(-2) M. The influence of the viscosity of the PDMS binder upon the internal oxygen supply is examined. The PDMS carbon-paste enzyme electrode displays a stable glucose response over prolonged (15 h) operation in an oxygen-free solution. On-line continuous testing indicates favorable dynamic properties with no carry-over effects over the physiological and pathophysiological range (3-12 mM glucose).
authors.affiliation: Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA. joewang@nmsu.edu
authors.forename: Joseph
authors.initials: J
authors.lastname: Wang
authors.forename: Shifang
authors.initials: S
authors.lastname: Li
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Mo
authors.forename: Josh
authors.initials: J
authors.lastname: Porter
authors.forename: Mustafa M
authors.initials: MM
authors.lastname: Musameh
authors.forename: Purnendu K
authors.initials: PK
authors.lastname: Dasgupta
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002244:Carbon; D004129:Dimethylpolysiloxanes / Q000737:chemistry* / Q000145:classification; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D009824:Ointments; D010100:Oxygen / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012828:Silicones / Q000737:chemistry* / Q000145:classification
chemical_list: D004129:Dimethylpolysiloxanes; D009824:Ointments; D012828:Silicones; C013830:baysilon; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose; D010100:Oxygen