Class 1 integron-associated tobramycin-gentamicin resistance in Campylobacter jejuni isolated from the broiler chicken house environment.
Using PCR, we screened 105 isolates of poultry-associated Campylobacter jejuni for the presence of class 1 integrons. Of those isolates, 21% (22 of 105) possessed the integrase gene, but only 5 isolates produced an amplicon in a 5'-3' conserved sequence PCR directed toward amplification of the resistance cassettes. DNA sequencing demonstrated that all five isolates possessed the aminoglycoside resistance gene, aacA4.
Lee
Margie D
MD
Department of Medical Microbiology and Parasitology. Athens Diagnostic Laboratory. Department of Environmental Health Sciences, The University of Georgia, Athens, Georgia 30602, USA. leem@vet.uga.edu
Sanchez
Susan
S
Zimmer
Martha
M
Idris
Umelaalim
U
Berrang
Mark E
ME
McDermott
Patrick F
PF
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002169:Campylobacter Infections / Q000382:microbiology* / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000187:drug effects*; D002645:Chickens / Q000382:microbiology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004797:Enzyme-Linked Immunosorbent Assay; D005839:Gentamicins / Q000494:pharmacology*; D032023:Integrons / Q000235:genetics*; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014031:Tobramycin / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D004269:DNA, Bacterial; D005839:Gentamicins; D014031:Tobramycin