A novel sialyl Lewis(x) analogue attenuates ischemia reperfusion injury in rabbit lung.
BACKGROUND
We investigated the effects of OJ-R9545, a novel Sialyl Lewis x analogue, on lung ischemia-reperfusion (IR) injury using an in vivo rabbit model.


METHODS
The left hilum of the lung was clamped for 110 minutes; the lung was then reperfused for 90 minutes. Either OJ-R9545 (10 mg/kg) or vehicle solution was administered from 10 minutes before reperfusion to 60 minutes after reperfusion in the OJ-R (+) and OJ-R (-) group (n = 6 in each group), respectively. The sham group (n = 3) underwent an identical procedure without ischemia.


RESULTS
Arterial oxygen tensions in the OJ-R (+) group were superior to those in the OJ-R (-) group from 30 to 90 minutes after reperfusion (p < 0.05 and p < 0.01). Lung wet/dry weight ratio and myeloperoxidase activity after reperfusion in the OJ-R (+) group were both significantly lower than the corresponding figures in the OJ-R (-) group (p < 0.05). The intrapulmonary leukocytes were significantly reduced in the OJ-R (+) group compared with those in the OJ-R (-) group (p < 0.01).


CONCLUSIONS
OJ-R9545 attenuates lung IR injury by preventing leukocyte infiltration into the lung.
Chen
Q
Q
Department of Thoracic Surgery, Faculty of Medicine, Kyoto University, Kyoto, Japan.
Yoshimura
T
T
Kawashima
M
M
Hanaoka
N
N
Fukuse
T
T
Bando
T
T
Wada
H
H
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D008297:Male; D009844:Oligosaccharides / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D011817:Rabbits; D011897:Random Allocation; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000201:enzymology; D000080506:Sialyl Lewis X Antigen
D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D009195:Peroxidase