Relationship between coffee husk caffeine degradation and respiration of Aspergillus sp. LPBx in solid-state fermentation.
Studies were carried out in a packed-bed column fermentor using coffee husk as substrate in order to verify a relationship between caffeine degradation and the respiration of Aspergillus sp. LPBx. Fermentation conditions were optimized by using factorial design experiments. The kinetic study showed that the caffeine degradation was related to the development of mold and its respiration and also with the consumption of reducing sugars present in coffee husk. From the values obtained experimentally for oxygen uptake rate and CO2 evolved, we determined a biomass yield of 3.811 g of biomass/g of consumed O2 and a maintenance coefficient of 0.0031 g of consumed O2/(g of biomass x h). The maximum caffeine degradation achieved was 90%.
Brand
Débora
D
Departamento Engenharia Quimica, Universidade Federal do Paraná, Curitiba, Brazil.
Pandey
Ashok
A
Rodriguez-Leon
Jose A
JA
Roussos
Sevastianos
S
Brand
Ivo
I
Soccol
Carlos R
CR
D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002110:Caffeine / Q000737:chemistry / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D019069:Cell Respiration; D003069:Coffee / Q000737:chemistry* / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D012107:Research Design; D014867:Water / Q000032:analysis
D003069:Coffee; D003470:Culture Media; D014867:Water; D002245:Carbon Dioxide; D002110:Caffeine; D010100:Oxygen