[The changes of rare codons increase expression level of SEA].
The rare codons of a fragment in staphylococcal enterotoxin A gene were turned into the most high usage frequency codons in E. coli by overlap PCR technique. Genes of sea and seam were cloned into 7ZTS expression vector and transformed into JM109(DE3), respectively. The result shows that expression level of sea gene was very low, but the expression level of seam was as high as 15% of total cell proteins. The expression product shows activity of antitumor in vivo.
Shi
Cheng-Bo
CB
ShenYang Institute of Applied Ecology, Chinese Academy of Science, ShenYang 110015, China.
Lu
An-Guo
AG
Wu
Wen-Fang
WF
Yang
Li-Quan
LQ
Feng
Jia-Xun
JX
Bai
Xue-Liang
XL
D000818:Animals; D001483:Base Sequence; D003062:Codon / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology; D017354:Point Mutation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology
D003062:Codon; D004768:Enterotoxins; D011994:Recombinant Proteins; C026066:enterotoxin A, Staphylococcal