title: Melting curve analysis of feline calicivirus isolates detected by real-time reverse transcription PCR.
abstract: A real-time reverse transcription polymerase chain reaction assay was developed to detect feline calicivirus infection in cats. Using SYBR green I melting curve analysis, isolates could be distinguished by their melting temperature. The assay is sensitive, linear over a wide range of template concentrations and allows accurate quantitation of viral load.
authors.affiliation: Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol BS40 5DU, UK. c.r.helps@bristol.ac.uk
authors.forename: Chris
authors.initials: C
authors.lastname: Helps
authors.forename: Philippa
authors.initials: P
authors.lastname: Lait
authors.forename: Séverine
authors.initials: S
authors.lastname: Tasker
authors.forename: Dave
authors.initials: D
authors.lastname: Harbour
mesh_terms: D000818:Animals; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017927:Calicivirus, Feline / Q000235:genetics / Q000302:isolation & purification*; D002371:Cat Diseases / Q000175:diagnosis* / Q000821:virology; D002415:Cats; D009691:Nucleic Acid Denaturation; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary