Altered renin synthesis and secretion in the kidneys of heterozygous mice with a null mutation in the TGF-beta(2) gene.
Transforming growth factors beta (TGF-betas) are peptides involved in autocrine and paracrine control of cell growth and differentiation. In the kidneys, TGF-beta(2) has been shown to localize specifically in renin-producing cells in various conditions stimulating the renin response. To test in vivo the functional role of TGF-beta(2), the renin response was investigated in mice heterozygous for a null mutation of the TGF-beta(2) gene, which had a twofold reduction in the amount of TGF-beta(2) mRNA. Although the increase in plasma renin concentration triggered by dehydration was not different from wild-type mice, renal renin mRNA and protein levels were higher in mutant mice under hydrated or dehydrated conditions. These data suggest that TGF-beta(2) exerts an inhibitory effect on renin synthesis and release from the juxtaglomerular apparatuses.
Pietri
Laurence
L
INSERM U367, Hôpital Broussais, Paris, France.
Bloch-Faure
May
M
Belair
Marie-France
MF
Sanford
L Philip
LP
Doetschman
Tom
T
Ménard
Joël
J
Bruneval
Patrick
P
Meneton
Pierre
P
D000818:Animals; D001160:Arterioles / Q000378:metabolism; D001834:Body Water / Q000378:metabolism; D003681:Dehydration / Q000378:metabolism; D005333:Fetus / Q000378:metabolism; D017353:Gene Deletion; D005838:Genotype; D006579:Heterozygote; D007606:Juxtaglomerular Apparatus / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D012079:Renal Circulation; D012083:Renin / Q000235:genetics / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000235:genetics*; D053781:Transforming Growth Factor beta2; D014868:Water Deprivation / Q000502:physiology
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D053781:Transforming Growth Factor beta2; D012083:Renin