title: Characterization of a new HLA-DRB3 allele, DRB3*0217, by direct sequencing.
abstract: We report the identification of a novel DRB3*02 using sequence-based typing (SBT). This new allele, officially named DRB3*0217, was detected while performing HLA high resolution typing of a bone marrow recipient and his siblings. DNA sequencing demonstrated the presence of a nucleotide substitution in exon 2 at position 199 where a C was substituted by a T. This point mutation at codon 67 (CTC-->TTC) has resulted in an amino acid substitution from Leucine to Phenylalanine.
authors.affiliation: Centro Transfusionale e di Immunologia dei Trapianti, Ospedale Maggiore, Policinico IRCCS, Milano, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Frison
authors.forename: E
authors.initials: E
authors.lastname: Longhi
authors.forename: F
authors.initials: F
authors.lastname: Poli
authors.forename: A
authors.initials: A
authors.lastname: Espadas de Arias
authors.forename: M
authors.initials: M
authors.lastname: Scalamogna
mesh_terms: D000483:Alleles; D019943:Amino Acid Substitution; D001483:Base Sequence; D006684:HLA-DR Antigens / Q000145:classification / Q000235:genetics*; D059826:HLA-DRB3 Chains; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D017354:Point Mutation
chemical_list: D006684:HLA-DR Antigens; D059826:HLA-DRB3 Chains