title: Expression of adhesion molecule T-cadherin is increased during neointima formation in experimental restenosis.
abstract: Phenotypic modulation, migration and proliferation of vascular smooth muscle cells (SMCs) are major events in restenosis after percutaneous transluminal angioplasty. Surface cell adhesion molecules, essential to morphogenesis and maintenance of adult tissue architecture, are likely to be involved, but little is known about cell adhesion molecules expressed on SMCs. T-cadherin is a glycosyl phosphatidylinositol-anchored member of the cadherin superfamily of adhesion molecules. Although highly expressed in vascular and cardiac tissues, its function in these tissues is unknown. We previously reported increased expression of T-cadherin in intimal SMCs in atherosclerotic lesions and proposed a role for T-cadherin in phenotype control. Here we performed immunohistochemical analysis of spatial and temporal changes in vascular T-cadherin expression following balloon catheterisation of the rat carotid artery. T-cadherin expression in SMCs markedly increases in the media early (1-4 days) after injury, and later (day 7-28) in forming neointima, especially in its preluminal area. Staining for monocyte/macrophage antigen ED-1, proliferating cell nuclear antigen and smooth muscle alpha-actin revealed that spatial and temporal changes in T-cadherin level coincided with the peak in cell migration and proliferation activity during neointima formation. In colchicine-treated cultures of rat aortic SMCs T-cadherin expression is increased in dividing M-phase cells but decreased in non-dividing cells. Together the data support an association between T-cadherin expression and SMC phenotype.
authors.affiliation: Laboratory for Molecular Endocrinology, Institute of Experimental Cardiology, 121552 Moscow, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Kudrjashova
authors.forename: Pavel
authors.initials: P
authors.lastname: Bashtrikov
authors.forename: Valery
authors.initials: V
authors.lastname: Bochkov
authors.forename: Yelena
authors.initials: Y
authors.lastname: Parfyonova
authors.forename: Vsevolod
authors.initials: V
authors.lastname: Tkachuk
authors.forename: Julia
authors.initials: J
authors.lastname: Antropova
authors.forename: Olga
authors.initials: O
authors.lastname: Iljinskaya
authors.forename: Eduard
authors.initials: E
authors.lastname: Tararak
authors.forename: Paul
authors.initials: P
authors.lastname: Erne
authors.forename: Danila
authors.initials: D
authors.lastname: Ivanov
authors.forename: Maria
authors.initials: M
authors.lastname: Philippova
authors.forename: Therese J
authors.initials: TJ
authors.lastname: Resink
mesh_terms: D000800:Angioplasty, Balloon / Q000009:adverse effects; D000818:Animals; D001011:Aorta / Q000166:cytology; D015820:Cadherins / Q000378:metabolism*; D020212:Carotid Artery Injuries / Q000378:metabolism / Q000473:pathology; D017536:Carotid Artery, Common / Q000378:metabolism* / Q000473:pathology; D016893:Carotid Stenosis / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011921:Rats, Inbred WKY; D017539:Tunica Intima / Q000378:metabolism* / Q000473:pathology
chemical_list: D015820:Cadherins; C099972:H-cadherin