title: The trans-spliced variants of Sp1 mRNA in rat.
abstract: trans-Splicing is the biological reaction that generates a mature mRNA from separate strands of pre-mRNAs. Previously, we reported that the trans-splicing between the two Sp1 pre-mRNA strands produced an mRNA with the exon 3-2-3 alignment in human HepG2 cells. Here we describe the rat counterpart as well as a newly identified variant with the exon 3-3 alignment in cultured rat cells. A qualitative evaluation of such alignments in poly(A)(+) RNA-rich preparation showed that both alignments arose from trans-splicing rather than circularization of a single strand. The identification of the trans-spliced products in both rat and human raises the possibility that trans-splicing on Sp1 pre-mRNA is rather common to mammals. It was observed that the level of the trans-spliced variants varies in different rat organs.
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro, Tokyo, 153-8902, Japan.
authors.forename: Terunao
authors.initials: T
authors.lastname: Takahara
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kasahara
authors.forename: Daisuke
authors.initials: D
authors.lastname: Mori
authors.forename: Shuichi
authors.initials: S
authors.lastname: Yanagisawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akanuma
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D040461:Gene Components; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012689:Sequence Homology, Nucleic Acid; D016329:Sp1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution; D020040:Trans-Splicing
chemical_list: D012333:RNA, Messenger; D016329:Sp1 Transcription Factor