The effect of oleanolic acid on sperm motion characteristics and fertility of male Wistar rats.
The study was designed to examine the effect of oleanolic acid on cauda epididymal sperm motion using a computer-aided sperm analysis system and to elucidate the relationship between sperm motion and fertility, as a tool for contraceptive studies. Oleanolic acid-polyvinylpyrrollidone suspension was orally administered to adult male Wistar rats for 30 days, followed by a 14-day drug withdrawal from half of the rats in the group. Control rats received only polyvinylpyrrollidone. All males were mated with untreated females. Treated males failed to impregnate females, whereas control and oleanolic acid withdrawn males achieved 100% pregnancies. Sperm motion analysed on the Sperm Motility Quantifier (SMQ) showed significant differences in linearity (P < 0.001) and wobble (P < 0.01) between control and treated groups. However, the curvilinear velocities were not significantly different (P > 0.05) among all the groups. Sperm motility patterns verified differences among kinematic parameters.
Mdhluli
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Diabetes Research Group: Primate Unit, South African Medical Research Council, PO Box 19070, Tygerberg 7505, South Africa.
van der Horst
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D000284:Administration, Oral; D000818:Animals; D003272:Contraceptive Agents, Male / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D005298:Fertility / Q000187:drug effects*; D007091:Image Processing, Computer-Assisted; D008297:Male; D009828:Oleanolic Acid / Q000008:administration & dosage / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D013078:Sperm Immobilizing Agents / Q000008:administration & dosage / Q000633:toxicity*; D013081:Sperm Motility / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
D003272:Contraceptive Agents, Male; D013078:Sperm Immobilizing Agents; D009828:Oleanolic Acid