title: Increased expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) and lymphocyte recruitment in murine gastritis induced by Helicobacter pylori.
abstract: Although T cell involvement in Helicobactor pylori-induced gastritis is known, mechanism about T cell recruitment is not understood. In this study we examined how mucosal addressin cell adhesion -molecule-1 (MAdCAM-1) is involved in lymphocyte recruitment in murine chronic gastritis induced by H. pylori. C57 BL/6 mice were infected with Sydney strain (SS1). Six months after infection, the stomach was removed. The expression of adhesion molecules, MAdCAM-1, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), and the cell surface antigens CD4, CD8, CD45R/B220 or beta7-integrin were determined by immunohistochemistry. A significant increase in CD4 lymphocytes was observed in the body portion of stomach in SS1-infected mice and most of these CD4 cells express beta7-integrin, a known counter ligand for MAdCAM-1 molecule. Strong MAdCAM-1 expression was observed adjacent to these cells in the lamina propria as well as in the submucosa of SS1-infected stomach. Quantitative analysis showed that the area of MAdCAM-1 expression well correlated with the infiltration of beta7-integrin positive lymphocytes. On the other hand, expression of ICAM-1 or VCAM-1 in the lamina propria was few even in the SS1-infected stomach. Increased expression of MAdCAM-1 was well correlated to the location of lymphocytes, which express CD4 and beta7-integrin. These results suggest the possibility that MAdCAM-1 may be largely involved in the lymphocyte recruitment in the gastritis mucosa with H. pylori.
authors.affiliation: Second Department of Internal Medicine, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hatanaka
authors.forename: R
authors.initials: R
authors.lastname: Hokari
authors.forename: K
authors.initials: K
authors.lastname: Matsuzaki
authors.forename: S
authors.initials: S
authors.lastname: Kato
authors.forename: A
authors.initials: A
authors.lastname: Kawaguchi
authors.forename: S
authors.initials: S
authors.lastname: Nagao
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Miyazaki
authors.forename: E
authors.initials: E
authors.lastname: Sekizuka
authors.forename: H
authors.initials: H
authors.lastname: Nagata
authors.forename: H
authors.initials: H
authors.lastname: Ishii
authors.forename: S
authors.initials: S
authors.lastname: Miura
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism; D002465:Cell Movement; D002908:Chronic Disease; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology / Q000473:pathology; D005756:Gastritis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000276:immunology* / Q000473:pathology; D016480:Helicobacter pylori; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000276:immunology; D007150:Immunohistochemistry; D039641:Integrin beta Chains / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009088:Mucoproteins / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D039641:Integrin beta Chains; C489082:Madcam1 protein, mouse; D009088:Mucoproteins; C091368:integrin beta7