Centrosome aberrations in acute myeloid leukemia are correlated with cytogenetic risk profile.
Genetic instability is a common feature in acute myeloid leukemia (AML). Centrosome aberrations have been described as a possible cause of aneuploidy in many human tumors. To investigate whether centrosome aberrations correlate with cytogenetic findings in AML, we examined a set of 51 AML samples by using a centrosome-specific antibody to pericentrin. All 51 AML samples analyzed displayed numerical and structural centrosome aberrations (36.0% +/- 16.6%) as compared with peripheral blood mononuclear cells from 21 healthy volunteers (5.2% +/- 2.0%; P <.0001). In comparison to AML samples with normal chromosome count, the extent of numerical and structural centrosome aberrations was higher in samples with numerical chromosome changes (50.5% +/- 14.2% versus 34.3% +/- 12.2%; P <.0001). When the frequency of centrosome aberrations was analyzed within cytogenetically defined risk groups, we found a correlation of the extent of centrosome abnormalities to all 3 risk groups (P =.0015), defined as favorable (22.5% +/- 7.3%), intermediate (35.3% +/- 13.1%), and adverse (50.3% +/- 15.6%). These results indicate that centrosome defects may contribute to the acquisition of chromosome aberrations and thereby to the prognosis in AML.
Neben
Kai
K
Medizinische Klinik und Poliklinik V, Universität Heidelberg, Germany.
Giesecke
Christian
C
Schweizer
Silja
S
Ho
Anthony D
AD
Krämer
Alwin
A
D000208:Acute Disease; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology; D016022:Case-Control Studies; D018385:Centrosome / Q000473:pathology*; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis; D006801:Humans; D007951:Leukemia, Myeloid / Q000209:etiology / Q000235:genetics / Q000473:pathology*; D012307:Risk Factors
D000911:Antibodies, Monoclonal; D000941:Antigens; C086178:pericentrin