Fine genetic mapping and physical delimitation of the lesion mimic gene Spl11 to a 160-kb DNA segment of the rice genome.
The rice lesion mimic mutant spl11 was previously found to confer broad-spectrum disease resistance to both Magnaporthe grisea and Xanthomonas oryzae pv. oryzae. To better understand the molecular basis underlying cell death and disease resistance in rice, a map-based cloning strategy has been employed to isolate Spl11. Five Spl11-linked RAPD markers were developed and four of them were mapped to rice chromosome 12. A high-resolution genetic map was developed using a segregating population consisting of 1138 lesion mimic individuals. Recombination suppression was observed in the vicinity of Spl11. Three molecular markers tightly linked to Spl11 were identified and used to screen a BAC library. A contig spanning the Spl11 locus was constructed and physical mapping delimited Spl11 to a 160-kb DNA segment within a single BAC clone. These results provide the essential information for the final isolation of this important gene in the rice defense pathway.
Zeng
L
L
Department of Plant Pathology, The Ohio State University, Kottman Hall, 2021 Coffey Road, Columbus, OH 43210-1087, USA.
Yin
Z
Z
Chen
J
J
Leung
H
H
Wang
G-L
GL
D017209:Apoptosis; D017343:Genes, Plant; D005819:Genetic Markers; D007113:Immunity, Innate / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D020161:Physical Chromosome Mapping; D019105:Random Amplified Polymorphic DNA Technique
D005819:Genetic Markers