[Purification of corneal endothelial antigen and anti-corneal endothelial antibody].
OBJECTIVE
To explore the methods for purifying corneal endothelial antigen (CEN-Ag) and preparing anti-corneal endothelial antibody (aCEN-Ab).


METHODS
Porcine corneal endothelia were obtained with the epithelia serving as control, CEN-Ag and corneal epithelial antigen (CEP-Ag) were isolated and purified by ion-exchange chromatography and affinity chromatography with 2B4.14.1 monoclonal antibody. The rabbits were immunized with the resultant CEN-Ag and CEP-Ag and their sera collected to harvest aCEN-Ab and anti-corneal epithelial antibody (aCEP-Ab) by affinity chromatography.


CONCLUSIONS
The relative molecular mass of CEN-Ag and CEP-Ag were 43 000 and 53 000 respectively as determined by SDS-PAGE, with their immunoglobulins being IgG2 and IgG3.
Tang
Na
N
Department of Ophthalmology, First Military Medical University, Guangzhou 510515, China.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004728:Endothelium, Corneal / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008970:Molecular Weight; D011817:Rabbits; D013552:Swine
D000941:Antigens; D007074:Immunoglobulin G