A general synthesis of specifically deuterated nucleotides for studies of DNA and RNA.
An efficient procedure is described for the preparation of ribonucleotides and deoxyribonucleotides with deuterium incorporated at the 1', 4', or 5' position. Three intermediates-[1-2H]-D-ribose, [4-2H]-D-ribose, and [5-2H(2)]-D-ribose-were prepared by chemical synthesis and subsequently converted to ribonucleotides and deoxyribonucleotides via enzymatic reactions. Milligram quantities of the desired products were obtained with an average deuterium content of 96+/-1%.
Chen
Bingzi
B
Department of Chemistry, Boston University, Boston, MA 02215, USA.
Jamieson
Elizabeth R
ER
Tullius
Thomas D
TD
D000818:Animals; D004247:DNA / Q000737:chemistry*; D003854:Deoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D003903:Deuterium / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods*; D010766:Phosphorylation; D012313:RNA / Q000737:chemistry*; D016337:RNA, Catalytic / Q000378:metabolism; D012264:Ribonucleotide Reductases / Q000737:chemistry; D012265:Ribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012266:Ribose / Q000737:chemistry; D013768:Tetrahymena / Q000378:metabolism
D003854:Deoxyribonucleotides; D007202:Indicators and Reagents; D016337:RNA, Catalytic; D012265:Ribonucleotides; D012313:RNA; D012266:Ribose; D004247:DNA; D003903:Deuterium; D012264:Ribonucleotide Reductases