Monitoring of a pyrite-oxidising bacterial population using DNA single-strand conformation polymorphism and microscopic techniques.
The single-strand conformation polymorphism (SSCP) technique was used to study the evolution of a bacterial consortium during the batch oxidation of a cobaltiferous pyrite in two types of bio-reactor: a bubble column and a classical stirred tank. Sequencing 16S rDNA revealed the presence of three organisms affiliated to Leptospirillum ferrooxidans, Acidithiobacillus thiooxidans and Sulfobacillus thermosulfidooxidans, respectively. Attempts were made to determine the proportions of bacteria attached to solid particles or freely suspended in the medium using a combination of PCR-SSCP and a microscopic technique. Ac. thiooxidans-related bacteria were dominant in the liquid during the early phase of the batch, but were later supplanted by L. ferrooxidans-related bacteria. L. ferrooxidans-related organisms were always in the majority on the solids. The growth of S. thermosulfidooxidans-related bacteria seemed to be favoured by the bubble-column reactor.
Battaglia-Brunet
F
F
BRGM, Environment and Process Division, Biotechnology Unit, 3 avenue Claude Guillemin, BP 6009, 45060 Orléans cedex 2, France, f.battaglia@brgm.fr
Clarens
M
M
D'Hugues
P
P
Godon
J J
JJ
Foucher
S
S
Morin
D
D
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019149:Bioreactors; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D004784:Environmental Monitoring; D007501:Iron / Q000378:metabolism*; D008906:Mining; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D013440:Sulfides / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013440:Sulfides; C011342:pyrite; D007501:Iron