Antigen-induced airway inflammation in the Brown Norway rat results in airway smooth muscle hyperplasia.
Asthma is characterized by chronic airways inflammation, airway wall remodeling, and airway hyperresponsiveness (AHR). An increase in airway smooth muscle has been proposed to explain a major part of AHR in asthma. We have used unbiased stereological methods to determine whether airway smooth muscle hyperplasia and AHR occurred in sensitized, antigen-challenged Brown Norway (BN) rats. Ovalbumin (OA)-sensitized BN rats chronically exposed to OA aerosol displayed airway inflammation and a modest level of AHR to intravenously administered ACh 24 h after the last antigen challenge. However, these animals did not show an increase in smooth muscle cell (SMC) number in the left main bronchus, suggesting that short-lived inflammatory mechanisms caused the acute AHR. In contrast, 7 days after the last aerosol challenge, there was a modest increase in SMC number, but no AHR to ACh. Addition of FCS to the chronic OA challenge protocol had no effect on the degree of inflammation but resulted in a marked increase in both SMC number and a persistent (7-day) AHR. These results raise the possibility that increases in airway SMC number rather than, or in addition to, chronic inflammation contribute to the persistent AHR detected in this model.
Xu
K F
KF
Bernard O'Brien Institute of Microsurgery, St. Vincent's Hospital, Fitzroy, Victoria 3065, Australia.
Vlahos
R
R
Messina
A
A
Bamford
T L
TL
Bertram
J F
JF
Stewart
A G
AG
D000109:Acetylcholine / Q000494:pharmacology; D000403:Airway Resistance; D000818:Animals; D001980:Bronchi / Q000187:drug effects / Q000473:pathology*; D016535:Bronchial Hyperreactivity / Q000276:immunology* / Q000503:physiopathology; D001991:Bronchitis / Q000276:immunology* / Q000473:pathology* / Q000503:physiopathology; D002452:Cell Count; D006965:Hyperplasia; D006969:Hypersensitivity, Immediate / Q000276:immunology / Q000503:physiopathology; D008297:Male; D009130:Muscle, Smooth / Q000473:pathology*; D010047:Ovalbumin / Q000276:immunology*; D051381:Rats; D011914:Rats, Inbred BN
D010047:Ovalbumin; D000109:Acetylcholine