title: V-1, a catecholamine biosynthesis regulatory protein, positively controls catecholamine secretion in PC12D cells.
abstract: Stably transfected PC12D cell lines overexpressing a catecholamine biosynthesis regulatory protein, V-1, were used to examine the functional role of V-1 in catecholamine secretion. High K(+)-induced dopamine secretion in V-1 overexpressing clones was shown to be markedly potentiated compared with control clones carried with a vector alone. As assayed intracellular calcium concentration ([Ca(2+)](i)) using fura-PE3, V-1 overexpression was observed to enhance high K(+)-elicited [Ca(2+)](i) elevation. Electron microscopic analysis revealed an increase in dense-cored vesicle formation by V-1 overexpression. These results suggest that the enhancement of high K(+)-induced dopamine secretion by V-1 overexpression results from the potentiation of high K(+)-induced [Ca(2+)](i) elevation and the increase in the number of dense-cored vesicles.
authors.affiliation: Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan. yamakuni@mail.pharm.tohoku.ac.jp
authors.forename: Tohru
authors.initials: T
authors.lastname: Yamakuni
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Yukisato
authors.initials: Y
authors.lastname: Ishida
authors.forename: Hideko
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Si Young
authors.initials: SY
authors.lastname: Song
authors.forename: Eijiro
authors.initials: E
authors.lastname: Adachi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Hiwatashi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ohizumi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004298:Dopamine / Q000096:biosynthesis* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C065700:myotrophin; D004298:Dopamine