A simple and effective NMR cell for studies of encapsulated proteins dissolved in low viscosity solvents.
Application of triple-resonance and isotope-edited-NOE methods to the study of increasingly larger macromolecules and their complexes remains a central goal of solution NMR spectroscopy. The slow reorientational motion of larger molecules leads to rapid transverse relaxation and results in losses in both resolution and sensitivity of multidimensional-multinuclear solution NMR experiments. A recently described technique employs a physical approach to increase the tumbling rate of macromolecules in an attempt to preserve access to the full range of structural restraints available to studies of smaller systems. This technique involves encapsulation of a hydrated protein in a surfactant shell which is subsequently solubilized in a low viscosity solvent. A simple, efficient and cost effective NMR cell that accommodates the moderate liquefaction pressures required in the encapsulation method is described. Application of the method to the 56 kD triose phosphate isomerase homodimer is demonstrated.
Flynn
Peter F
PF
The Johnson Research Foundation and Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104-6059, USA.
Milton
Mark J
MJ
Babu
Charles R
CR
Wand
A Joshua
AJ
D004058:Diffusion; D004339:Drug Compounding; D004867:Equipment Design; D009038:Motion; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D012997:Solvents / Q000494:pharmacology*; D014783:Viscosity; D014867:Water / Q000737:chemistry
D011506:Proteins; D012997:Solvents; D014867:Water