title: Histone chaperone ASF1 cooperates with the Brahma chromatin-remodelling machinery.
abstract: De novo chromatin assembly into regularly spaced nucleosomal arrays is essential for eukaryotic genome maintenance and inheritance. The Anti-Silencing Function 1 protein (ASF1) has been shown to be a histone chaperone, participating in DNA-replication-coupled nucleosome assembly. We show that mutations in the Drosophila asf1 gene derepress silencing at heterochromatin and that the ASF1 protein has a cell cycle-specific nuclear and cytoplasmic localization. Furthermore, using both genetic and biochemical methods, we demonstrate that ASF1 interacts with the Brahma (SWI/SNF) chromatin-remodelling complex. These findings suggest that ASF1 plays a crucial role in both chromatin assembly and SWI/SNF-mediated chromatin remodelling.
authors.affiliation: Department of Zoology and Animal Biology, University of Geneva, 1211 Geneva 4, Switzerland.
authors.forename: Yuri M
authors.initials: YM
authors.lastname: Moshkin
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Armstrong
authors.forename: Robert K
authors.initials: RK
authors.lastname: Maeda
authors.forename: John W
authors.initials: JW
authors.lastname: Tamkun
authors.forename: Peter
authors.initials: P
authors.lastname: Verrijzer
authors.forename: James A
authors.initials: JA
authors.lastname: Kennison
authors.forename: Francois
authors.initials: F
authors.lastname: Karch
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000302:isolation & purification / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D003433:Crosses, Genetic; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D005127:Eye Color / Q000502:physiology; D005260:Female; D017353:Gene Deletion; D020868:Gene Silencing; D006579:Heterozygote; D066298:In Vitro Techniques; D008297:Male; D018832:Molecular Chaperones / Q000502:physiology*; D009154:Mutation; D009707:Nucleosomes / Q000378:metabolism*; D021381:Protein Transport; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; D018832:Molecular Chaperones; D009707:Nucleosomes; C445179:SWI-SNF-B chromatin-remodeling complex; D014157:Transcription Factors; C491642:asf1 protein, Drosophila