title: Local presentation of Steel factor increases expression of c-kit immunoreactive interstitial cells of Cajal in culture.
abstract: The binding of Steel factor (SF) to c-kit initiates a signaling pathway essential for development and maintenance of interstitial cells of Cajal (ICC). Soluble and membrane-bound isoforms of SF are expressed in the gastrointestinal tract, but the role for either isoform in supporting ICC development is unknown. The aim of this study was to determine the role of SF in supporting ICC in culture. ICC were cultured from dissociated mouse jejunum and grown with fibroblast cell lines that produced either soluble, membrane-bound or membrane-restricted SF. ICC were identified and counted by c-kit immunoreactivity. The number of c-kit immunoreactive cells was greater in the coculture system compared with cultures grown without SF-producing fibroblasts. All forms of SF-producing fibroblasts increased ICC number in culture but physical separation of the fibroblasts from the c-kit immunoreactive cells, the addition of exogenous SF to the culture medium, or fibroblast-conditioned media did not. These results are consistent with the hypothesis that the membrane-bound form of SF preferentially contributes to expression of c-kit-positive ICC under cell culture conditions.
authors.affiliation: Enteric NeuroScience Program, Department of Physiology and Biophysics, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA. adam.rich@bms.com
authors.forename: Adam
authors.initials: A
authors.lastname: Rich
authors.forename: Steven M
authors.initials: SM
authors.lastname: Miller
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Gibbons
authors.forename: John
authors.initials: J
authors.lastname: Malysz
authors.forename: Joseph H
authors.initials: JH
authors.lastname: Szurszewski
authors.forename: G
authors.initials: G
authors.lastname: Farrugia
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology / Q000187:drug effects; D007583:Jejunum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019009:Proto-Oncogene Proteins c-kit / Q000502:physiology*; D019089:Stem Cell Factor / Q000494:pharmacology*
chemical_list: D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit