Effects of volatile aromatics, aldehydes, and phenols in tobacco smoke on viability and proliferation of mouse lymphocytes.
Thirteen chemicals present in tobacco smoke were assessed for their effect on viability and proliferation of mouse lymphocytes in vitro. Acetaldehyde, benzene, butyraldehyde, isoprene, styrene, and toluene produced no effect on either viability or proliferation after 3 h of exposure. Formaldehyde, catechol, acrylonitrile, propionaldehyde, and hydroquinone significantly inhibited T-lymphocyte and B-lymphocyte proliferation with IC50 values ranging from 1.19 x 10(-5) M to 8.20 x 10(-4) M after 3 h of exposure. Acrolein and crotonaldehyde not only inhibited T-cell and B-cell proliferation, but also acted on viability with IC50 values ranging from 2.06 x 10(5) M to 4.26 x 10(-5) M. Mixtures of acrolein, formaldehyde, and propionaldehyde or crotonaldehyde were tested and interactive effects at 0.5 and 1 x IC50 were observed. Two mixtures significantly inhibited T-cell proliferation when compared to the control at 0.1 x IC50 concentration. The present study shows that some chemicals known to be present in tobacco smoke exert an effect on lymphocyte viability and proliferation in vitro.
Poirier
Martin
M
INRS-Institut Armand Frappier, Pointe-Claire, Quebec, Canada.
Fournier
Michel
M
Brousseau
Pauline
P
Morin
André
A
D000447:Aldehydes / Q000633:toxicity*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D005260:Female; D005434:Flow Cytometry; D006841:Hydrocarbons, Aromatic / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D010636:Phenols / Q000633:toxicity*; D012906:Smoke / Q000009:adverse effects* / Q000032:analysis; D014835:Volatilization
D000447:Aldehydes; D004338:Drug Combinations; D006841:Hydrocarbons, Aromatic; D010636:Phenols; D012906:Smoke