Genetic integrity of ex situ Genebank collections.
The genetic integrity of four accessions of the cross-pollinating species rye (Secale cereale L.) was investigated. Seeds available from the first and most recent regeneration cycles, multiplied 8, 12 (twice) or 14 times were fingerprinted using microsatellite markers. In all four accessions the allele numbers and frequencies changed after regeneration. Alleles present in the original seed sample were not detectable in the regenerated populations, whereas on the other hand, alleles were found in the recent seed sample, which were not observed in the investigated plants of the original one.
Chebotar
Sabina
S
Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, D06466 Gatersleben, Germany.
Röder
Marion S
MS
Korzun
Victor
V
Börner
Andreas
A
D000483:Alleles; D018070:Biological Specimen Banks; D018556:Crops, Agricultural / Q000235:genetics / Q000592:standards*; D003433:Crosses, Genetic; D005787:Gene Frequency; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D009154:Mutation; D011058:Pollen; D012434:Secale / Q000235:genetics*; D012639:Seeds
D005819:Genetic Markers