title: Cryopreservation prevents arterial allograft dilation.
abstract: Historically, immune-mediated degradation and subsequent aneurysm formation have limited the usefulness of cryopreserved arterial allografts. This study tested the hypothesis that modern cryopreserved arterial allografts are protected from immune-mediated dilation. Abdominal aortas were harvested from anesthetized rats (Lewis and Brown-Norway) for immediate implantation or cryopreservation. Subsequently, Lewis rats underwent infrarenal aortic replacement with either an acutely harvested or a cryopreserved graft. There were four experimental groups: (1) acutely harvested isografts (Iso; n = 6), (2) cryopreserved isografts (C-Iso; n = 6), (3) cryopreserved allografts (C-Allo; n = 6), and (4) acutely harvested allografts (Allo; n = 6). All grafts were explanted at 8 weeks. A video camera and edge detection software were used to measure systolic and diastolic in vivo graft diameter (d). Measurement of arterial blood pressure (p) allowed calculation of compliance (Dd/Dp). Tail-cuff plethysmography was used to assess graft patency at 1 week. Graft diameter and blood pressure measurements were repeated at harvest. All harvested grafts were examined histologically. Our results showed that cryopreservation prevented immune-mediated dilation in arterial allografts in our 8-week rat implant model. Furthermore, the compliance of the cryopreserved grafts and was similar to that of controls. Further investigation is needed to delineate the exact mechanism of these potential clinically significant findings.
authors.affiliation: Division of Vascular Surgery, Department of Surgery, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Medical Science Building Room 2363, Cincinnati, OH 45267-0558, USA. Joseph.Giglia@UC.edu
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Giglia
authors.forename: Jeremy D
authors.initials: JD
authors.lastname: Ollerenshaw
authors.forename: Patti E
authors.initials: PE
authors.lastname: Dawson
authors.forename: Kirby S
authors.initials: KS
authors.lastname: Black
authors.forename: William M
authors.initials: WM
authors.lastname: Abbott
mesh_terms: D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000503:physiopathology / Q000517:prevention & control / Q000601:surgery; D015415:Biomarkers / Q000032:analysis; D001794:Blood Pressure / Q000502:physiology; D015925:Cryopreservation; D004108:Dilatation, Pathologic / Q000503:physiopathology / Q000517:prevention & control / Q000601:surgery; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D014184:Transplantation, Homologous; D014654:Vascular Patency / Q000502:physiology
chemical_list: D015415:Biomarkers