Prostaglandin D2 generation by rat peritoneal mast cells stimulated with Datura stramonium agglutinin and its inhibition by haptenic sugar and wheat germ agglutinin.
The production of prostaglandin D2 (PGD2) by rat peritoneal mast cells incubated with N-acetyl glucosamine (GlcNAc) oligomer-specific Datura stramonium agglutinin (DSA) for 10 min in the presence of 0.3 mM Ca2+ was examined. Previously, our group reported that the incubation of rat mast cells with DSA (5 - 100 microg/ml) under similar conditions resulted in a calcium influx and histamine release via a pertussis toxin-sensitive G-protein pathway of the mast cells, and the histamine release was inhibited by haptenic sugar chitooligosaccharides or GlcNAc-specific lectin wheat germ agglutinin (WGA) (K. Matsuda et al., Jpn J Pharmacol 66, 195 - 204 (1994)). DSA (5 - 100 microg/ml) dose-dependently stimulated the mast cells to generate PGD2. Chitooligosaccharides (1% w/v) and WGA (100 microg/ml) inhibited the production of PGD2 induced by 100 microg/ml of DSA, suggesting that the effect of DSA is sugar-specific. A prostaglandin G/H synthase inhibitor NS-398 (N-[cyclohexyloxy-4-nitrophenyl] methanesulfonamide) (10 microM) inhibited the formation of PGD2 induced by DSA (20 microg/ml). These results suggest that the binding of DSA to the corresponding sugar residues on the mast cell surface mediates the signaling of the prostaglandin G/H synthase pathway.
Suzuki-Nishimura
Tamiko
T
Department of Pharmacology, Meiji Pharmaceutical University, Tokyo, Japan. nishimura@kiko.go.jp
Uchida
Masaatsu K
MK
D000117:Acetylglucosamine / Q000494:pharmacology; D000818:Animals; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D013286:Datura stramonium; D006241:Haptens / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D009844:Oligosaccharides / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000502:physiology; D037121:Plant Lectins / Q000494:pharmacology*; D015230:Prostaglandin D2 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014909:Wheat Germ Agglutinins / Q000494:pharmacology*
D016861:Cyclooxygenase Inhibitors; C041384:Datura stramonium lectin; D006241:Haptens; D009844:Oligosaccharides; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins; D015230:Prostaglandin D2; D000117:Acetylglucosamine