Determination of proteins at nanogram levels by their quenching effect on the chemiluminescence reaction between luminol and hydrogen peroxide with manganese-tetrasulfonatophthalocyanine as a new catalyst.
The manganese-tetrasulfonatophthalocyanine (MnTSPc) catalyzed luminol-hydrogen peroxide chemiluminescence (CL) systems can be quenched in the presence of proteins. A highly sensitive CL quenching method has been developed for the determination of proteins. Under optimum conditions, the linear ranges of the calibration curves were 0.1-20 microg/mL for human serum albumin (HSA), 0.2-20 microg/mL for human gamma-IgG, and 0.5-50 microg/mL for the bovine serum albumin (BSA) with the corresponding detection limits were 1.9 ng/mL, 2.7 ng/mL, and 3.4 ng/mL. The method has been applied to the analysis of total proteins in human serum samples and the results were in good agreement with clinical data provided.
Li
Yongxin
Y
College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, the People's Republic of China.
Zhao
Danhua
D
Zhu
Changqin
C
Wang
Lun
L
Xu
Jingou
J
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002417:Cattle; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007142:Immunoglobulin gamma-Chains / Q000032:analysis; D007211:Indoles / Q000737:chemistry*; D008163:Luminescent Measurements; D009942:Organometallic Compounds / Q000737:chemistry*; D010634:Phenobarbital / Q000737:chemistry*; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000032:analysis
D001798:Blood Proteins; D007142:Immunoglobulin gamma-Chains; D007211:Indoles; D009942:Organometallic Compounds; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; C052739:manganese tetrasulfophthalocyanine; D006861:Hydrogen Peroxide; D010634:Phenobarbital