title: A novel single-stranded DNA expression vector.
abstract: A novel expression vector system has been developed that can intracellularly generate any single-stranded DNA (ssDNA) molecule, such as a triplex-forming oligonucleotide (TFO), antisense oligodeoxynucleotide (ODN) and DNA enzyme, by our laboratory at CytoGenix, Inc. 'Proof of concept' studies from our laboratory as well as our collaborators' indicate that this ssDNA expression vector system is capable of producing, intracellularly, antisense ODNs, DNA enzymes or TFOs for the purpose of downregulating gene expression or inducing targeted genome modification. This technology provides new research tools and has potential applications in gene target validation and drug development.
authors.affiliation: CytoGenix, Inc., 9881 S. Wilcrest Drive, Houston, TX 77099, USA. ychen@cytogenix.com
authors.forename: Yin
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D019908:Proto-Oncogene Proteins c-raf / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004277:DNA, Single-Stranded; D019908:Proto-Oncogene Proteins c-raf; D001616:beta-Galactosidase