title: Improved procedure for the separation of major stratum corneum lipids by means of automated multiple development thin-layer chromatography.
abstract: The separation of the major stratum corneum lipids, i.e., ceramides, fatty acids, cholesterol and its esters by means of high-performance thin-layer chromatography is hereby presented. The used automated multiple development technique allows the reproducible development of a 17-step solvent gradient also capable of separating seven ceramide classes in the same run. Reliable quantification has been performed after visualisation and densitometric scanning. The present approach is less time and solvent-consuming than previously described procedures. The application to samples obtained by in vivo skin surface extraction with hexane-ethanol (2:1) demonstrates that the method can be routinely used for diagnostic purposes.
authors.affiliation: Institute of Pharmaceutics and Biopharmaceutics, Martin Luther University, W-Langenbeck-Str 4, 06120 Halle (S), Germany.
authors.forename: Hany
authors.initials: H
authors.lastname: Farwanah
authors.forename: Reinhard
authors.initials: R
authors.lastname: Neubert
authors.forename: Sebastian
authors.initials: S
authors.lastname: Zellmer
authors.forename: Klaus
authors.initials: K
authors.lastname: Raith
mesh_terms: D000328:Adult; D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000302:isolation & purification*; D008297:Male; D012867:Skin / Q000737:chemistry*
chemical_list: D008055:Lipids