Identification of new thyroid hormone-regulated genes in rat brain neuronal cultures.
As a first approach to study the molecular mechanisms that underlie the effects of thyroid hormones on the developing brain, we used a cDNA microarray technology to identify early thyroid hormone-regulated genes in brain neuronal cultures treated with tri-iodothyronine (T3) for 3 h. We identified three genes that were up-regulated by T3, basic transcription element-binding protein, nuclear pore glycoprotein and bone morphogenetic protein-4 and one that was down-regulated, the neuronal apoptosis-inducing gene. We confirmed that these genes were also regulated by the thyroid state in the developing brain. Our findings enrich our knowledge of signaling pathways regulated by thyroid hormones and open new avenues for studying the molecular mechanisms of thyroid hormones in the developing brain.
Martel
Julie
J
Laboratory of Human Genetics, Laval University Medical Research Center, Quebec, Canada.
Cayrou
Christelle
C
Puymirat
Jack
J
D000818:Animals; D000831:Animals, Newborn; D051017:Apoptosis Regulatory Proteins; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics*; D005260:Female; D005333:Fetus; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D051741:Kruppel-Like Transcription Factors; D008562:Membrane Glycoproteins / Q000235:genetics; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009479:Neuropeptides / Q000235:genetics; D028861:Nuclear Pore Complex Proteins; D011247:Pregnancy; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013963:Thyroid Hormones / Q000378:metabolism* / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics; D014284:Triiodothyronine / Q000378:metabolism / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics*
D051017:Apoptosis Regulatory Proteins; C525810:Bmp4 protein, mouse; C525811:Bmp4 protein, rat; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D004268:DNA-Binding Proteins; C484585:Hrk protein, rat; C489675:Klf9 protein, mouse; D051741:Kruppel-Like Transcription Factors; D008562:Membrane Glycoproteins; D009479:Neuropeptides; D028861:Nuclear Pore Complex Proteins; D012333:RNA, Messenger; D013963:Thyroid Hormones; D014157:Transcription Factors; C049312:nuclear pore protein p62; D014284:Triiodothyronine; C077017:Klf9 protein, rat