Irradiation-induced apoptosis of mouse bone marrow stromal cells.
OBJECTIVE
To study the apoptotic patterns of bone marrow stromal cells (BMSCs) after irradiation.


METHODS
Apoptosis of BMSCs induced by (60)Co gamma irradiation was detected by both flow cytometry and electron microscopy.


RESULTS
Compared with normal control group, no manifest changes in the apoptosis ratio occurred in BMSCs after 50 Gy irradiation (P>0.05) as demonstrated by flow cytometry. Four hours after 100 Gy irradiation, manifest changes in apoptosis ratio of BMSCs were observed (P<0.01), reaching the peak 20 h later. Similar apoptotic patterns occurred in BMSCs exposed to irradiation of 200 Gy (P>0.05), only the apoptosis ratio was slightly higher.


CONCLUSIONS
Irradiation of proper dose may induce apoptosis in vitro in BMSCs that possess strong resistance to irradiation.
Yang
Jun
J
Department of Teaching Affairs, First Military Medical University, Guangzhou 510515, China.
Ding
Zhen-Hua
ZH
Luo
Cheng-Ji
CJ
He
Pei
P
Zheng
Li
L
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D001854:Bone Marrow Cells / Q000528:radiation effects* / Q000648:ultrastructure; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D005720:Gamma Rays; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D017154:Stromal Cells / Q000528:radiation effects* / Q000648:ultrastructure