title: Proteasome-mediated degradation antagonizes critical levels of the apoptosis-inducing C1D protein.
abstract: The C1D gene is expressed in a broad spectrum of mammalian cells and tissues but its product induces apoptotic cell death when exceeding a critical level. Critical levels are achieved in a fraction of cells by transient transfection with EGFP-tagged C1D expression constructs. However, transfected cells expressing sub-critical levels of C1D(EGFP) escape apoptotic cell death by activation of a proteasome-mediated rescue mechanism. Inhibition of the proteasome-dependent degradation of the C1D(EGFP) protein results in a parallel increase of the intracellular C1D level and in the fraction of apoptotic cells.
authors.affiliation: Division Biochemistry of the Cell (B0300), German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany. werner@dkfz.de
authors.forename: Karsten
authors.initials: K
authors.lastname: Rothbarth
authors.forename: Hermann
authors.initials: H
authors.lastname: Stammer
authors.forename: Dieter
authors.initials: D
authors.lastname: Werner