title: The activation of nuclear phosphoinositide 3-kinase C2beta in all-trans-retinoic acid-differentiated HL-60 cells.
abstract: The activity of nuclear phosphoinositide 3-kinase C2beta (PI3K-C2beta) was investigated in HL-60 cells induced to differentiate along granulocytic or monocytic lineages. A significant increase in the activity of immunoprecipitated PI3K-C2beta was observed in the nuclei and nuclear envelopes isolated from all-trans-retinoic acid (ATRA)-differentiated cells which was inhibited by the presence of PI3K inhibitor LY 294002. High-performance liquid chromatography analysis of inositol lipids showed an increased incorporation of radiolabelled phosphate in both PtdIns(3)P and PtdIns(3,4,5)P(3) with no changes in the levels of PtdIns(4)P, PtdIns(3,4)P(2) and PtdIns(4,5)P(2). Western blot analysis of the PI3K-C2beta immunoprecipitates with anti-P-Tyr antibody revealed a significant increase in the level of the immunoreactive band corresponding to PI3K-C2beta in the nuclei and nuclear envelopes isolated from ATRA-differentiated cells.
authors.affiliation: Department of Physiology and Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Salata 3, 10000 Zagreb, Croatia.
authors.forename: Dora
authors.initials: D
authors.lastname: Visnjić
authors.forename: Vladiana
authors.initials: V
authors.lastname: Crljen
authors.forename: Josip
authors.initials: J
authors.lastname: Curić
authors.forename: Drago
authors.initials: D
authors.lastname: Batinić
authors.forename: Stefano
authors.initials: S
authors.lastname: Volinia
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Banfić
mesh_terms: D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects*; D002467:Cell Nucleus / Q000201:enzymology*; D004789:Enzyme Activation; D018922:HL-60 Cells; D006801:Humans; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D014212:Tretinoin; D019869:Phosphatidylinositol 3-Kinases