title: Molecular cloning and characterization of a novel carboxylesterase-like protein that is physiologically present at high concentrations in the urine of domestic cats (Felis catus).
abstract: Normal mammals generally excrete only small amounts of protein in the urine, thus avoiding major leakage of proteins from the body. Proteinuria is the most commonly recognized abnormality in renal disease. However, healthy domestic cats ( Felis catus ) excrete proteins at high concentrations (about 0.5 mg/ml) in their urine. We investigated the possible cause of proteinuria in healthy cats, and discovered a 70 kDa glycoprotein, which was excreted as a major urinary protein in cat urine, irrespective of gender. To elucidate the biochemical functions and the excretion mechanism of this protein, we cloned the cDNA for this protein from a cat kidney cDNA library. The deduced amino acid sequence shared 47% identity with the rat liver carboxylesterase (EC 3.1.1.1), and both the serine hydrolase active site and the carboxylesterase-specific sequence were conserved. Therefore we named this protein cauxin (carboxylesterase-like urinary excreted protein). In contrast to the mammalian carboxylesterases, most of which are localized within the cells of various organs, cauxin was expressed specifically in the epithelial cells of the distal tubules, and was secreted efficiently into the urine, probably because it lacked the endoplasmic reticulum retention sequence (HDEL). Based on our finding that cauxin is not expressed in the immature cat kidney, we conclude that cauxin is involved in physiological functions that are specific for mature cats. Recently, cauxin-like cDNAs were found from human brain and teratocarcinoma cells. These data suggest that cauxin and cauxin-like human proteins are categorized as a novel group of carboxylesterase multigene family.
authors.affiliation: Department of Agro-bioscience, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Kamiie
authors.forename: Satoshi
authors.initials: S
authors.lastname: Soeta
authors.forename: Hideharu
authors.initials: H
authors.lastname: Taira
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yamashita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D043182:Carboxylesterase / Q000652:urine*; D002265:Carboxylic Ester Hydrolases / Q000652:urine*; D002415:Cats; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C515680:cauxin protein, Felis catus