title: The ratio of plasminogen activator inhibitor type-1 activity to the concentration of plasminogen activator inhibitor type-1 protein in diabetes: adding insult to injury.
abstract: BACKGROUND
  This study was performed to delineate the relationship between plasminogen activator inhibitor type-1 (PAI-1) activity and PAI-1 protein to characterize the functional importance of elevated PAI-1 in diabetes.
  
  
  METHODS
  PAI-1 activity (chromogenic substrate kinetic assay) and protein (enzyme-linked immunosorbent assay) were assayed in baseline central venous catheter-acquired samples from 170 patients with type 2 diabetes studied in the Troglitazone Atherosclerosis Regression Trial (TART).
  
  
  RESULTS
  The data demonstrated that the ratio of PAI-1 activity to the concentration of PAI-1 protein is increased as a function of increasing concentrations of PAI-1 protein in blood.
  
  
  CONCLUSIONS
  The results demonstrate that the ratio of PAI-1 activity to the concentration of PAI-1 protein is higher when the concentration of PAI-1 is elevated. The nonlinearity appears to depend on the fact that the rate of disappearance of PAI-1 activity in blood is a function of the concentration of PAI-1 protein.
authors.affiliation: Department of Medicine, University of Vermont College of Medicine, Burlington, Vermont, USA. burt.sobel@vtnednet.org
authors.forename: Burton E
authors.initials: BE
authors.lastname: Sobel
authors.forename: Dagnija
authors.initials: D
authors.lastname: Neimane
authors.forename: Wendy J
authors.initials: WJ
authors.lastname: Mack
authors.forename: Howard N
authors.initials: HN
authors.lastname: Hodis
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Buchanan
mesh_terms: D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood*; D010960:Plasminogen Activators / Q000097:blood; D015842:Serine Proteinase Inhibitors / Q000097:blood*; D010959:Tissue Plasminogen Activator / Q000097:blood
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D015842:Serine Proteinase Inhibitors; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator