title: Mass spectrometric identification of isoforms of PR proteins in xylem sap of fungus-infected tomato.
abstract: The protein content of tomato (Lycopersicon esculentum) xylem sap was found to change dramatically upon infection with the vascular wilt fungus Fusarium oxysporum. Peptide mass fingerprinting and mass spectrometric sequencing were used to identify the most abundant proteins appearing during compatible or incompatible interactions. A new member of the PR-5 family was identified that accumulated early in both types of interaction. Other pathogenesis-related proteins appeared in compatible interactions only, concomitantly with disease development. This study demonstrates the feasibility of using proteomics for the identification of known and novel proteins in xylem sap, and provides insights into plant-pathogen interactions in vascular wilt diseases.
authors.affiliation: Plant Pathology, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands. rep@science.uva.nl
authors.forename: Martijn
authors.initials: M
authors.lastname: Rep
authors.forename: Henk L
authors.initials: HL
authors.lastname: Dekker
authors.forename: Jack H
authors.initials: JH
authors.lastname: Vossen
authors.forename: Albert D
authors.initials: AD
authors.lastname: de Boer
authors.forename: Petra M
authors.initials: PM
authors.lastname: Houterman
authors.forename: Dave
authors.initials: D
authors.lastname: Speijer
authors.forename: Jaap W
authors.initials: JW
authors.lastname: Back
authors.forename: Chris G
authors.initials: CG
authors.lastname: de Koster
authors.forename: Ben J C
authors.initials: BJ
authors.lastname: Cornelissen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D005670:Fusarium / Q000254:growth & development*; D043326:Glucan 1,3-beta-Glucosidase; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism; D007113:Immunity, Innate; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018514:Plant Structures / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D020033:Protein Isoforms; C053376:pathogenesis-related proteins, plant; D006026:Glycoside Hydrolases; D043326:Glucan 1,3-beta-Glucosidase; C117648:beta-1,3-exoglucanase