[Role of the three-dimensional distribution of abl and bcr genes in the formation of bcr/abl fusion gene in interphase neucleus].
OBJECTIVE
To explore the mechanism of bcr/abl fusion gene formation in view of its biological stereology.


METHODS
Assisted by fluorescent in situ hybridization combined with confocal laser scanning microscopy, we observed the effect of gamma-ray exposure on three-dimensional distribution of bcr and abl genes in the interphase nucleus of IM-9 cell line.


RESULTS
In interphase nuclei of IM-9 cells, abl and bcr genes retained their own definite distribution patterns that were dynamically and regularly modulated along with the phases of the cell cycle. gamma-ray exposure, however, produced shortened distances between the 2 genes.


CONCLUSIONS
The accessibility of abl and bcr genes to each other in the interphase nuclei is one of the factors for bcr/acl fusion gene formation.
Zhang
Qing
Q
Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. qingzhang@vip.163.com
Zhou
Shu-Yun
SY
Liu
Xiao-Li
XL
Niu
Chao
C
Xu
Lan
L
Chen
Sai-Juan
SJ
D002453:Cell Cycle / Q000235:genetics; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics* / Q000528:radiation effects; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D016313:Genes, abl / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase / Q000235:genetics / Q000528:radiation effects; D018613:Microscopy, Confocal; D015513:Oncogene Proteins / Q000235:genetics*; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D051562:Proto-Oncogene Proteins c-bcr
D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D016044:Fusion Proteins, bcr-abl; C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr