[Correlation of protein kinase C isoform expression with multidrug resistance in KBV200 cells].
OBJECTIVE
To investigate the association of protein kinase C (PKC) isoforms and multidrug resistance (MDR) mechanism in KBV200 cells.


METHODS
Western blotting was utilized to examine the expression and subcellular distribution of PKC isoforms were measured in KBV200 cells with MDR and drug-sensitive KB cells, and the fluorescence intensity of PKC isoforms was detected by flow cytometry (FCM).


RESULTS
KBV200 cells possessed higher PKC activities, with increased percentage of membrane fraction. As compared with parental KB cells, the expression of PKCalpha was significantly increased in KBV200 cells, while PKCbeta and epsilon expressions remained unchanged, and PKCgamma and zeta failed to be detected. Fluorescence intensity of PKCalpha in KBV200 cells was increased.


CONCLUSIONS
PKC might contribute to MDR phenotype in KBV200 cells, and of the isoforms so far detected, PKCalpha might play an important role in MDR phenotype.
Yuan
Ya-Wei
YW
Department of Radiotherapy, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China. ywyuan@163.net
Sun
Ai-Min
AM
Li
Chuan-Gang
CG
Chen
Jun
J
Qiu
Peng-Xin
PX
D015153:Blotting, Western; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
D007527:Isoenzymes; D011493:Protein Kinase C