H(2)O(2) generation during the auto-oxidation of coniferyl alcohol drives the oxidase activity of a highly conserved class III peroxidase involved in lignin biosynthesis.
Characterization of lignified Zinnia elegans hypocotyls by both alkaline nitrobenzene oxidation and thioacidolysis reveals that coniferyl alcohol units are mainly found as part of 4-O-linked end groups and aryl-glycerol-beta-aryl ether (beta-O-4) structures. Z. elegans hypocotyls also contain a basic peroxidase (EC 1.11.1.7) capable of oxidizing coniferyl alcohol in the absence of H(2)O(2). Results showed that the oxidase activity of the Z. elegans basic peroxidase is stimulated by superoxide dismutase, and inhibited by catalase and anaerobic conditions. Results also showed that the oxidase activity of this peroxidase is due to an evolutionarily gained optimal adaptation of the enzyme to the microM H(2)O(2) concentrations generated during the auto-oxidation of coniferyl alcohol, the stoichiometry of the chemical reaction (mol coniferyl alcohol auto-oxidized/mol H(2)O(2) formed) being 0.496. These results therefore suggest that the H(2)O(2) generated during the auto-oxidation of coniferyl alcohol is the main factor that drives the unusual oxidase activity of this highly conserved lignin-synthesizing class III peroxidase.
Pomar
Federico
F
Department of Plant Biology (Plant Physiology), University of Murcia, E-30100 Murcia, Spain.
Caballero
Nuria
N
Pedreño
María
M
Ros Barceló
A
A
D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008031:Lignin / Q000096:biosynthesis*; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*
D010636:Phenols; D008031:Lignin; D006861:Hydrogen Peroxide; C010559:coniferyl alcohol; D010544:Peroxidases