title: Hospital-acquired Aspergillus fumigatus infection: can molecular typing methods identify an environmental source?
abstract: Aspergillus fumigatus infection in hospitalized immunocompromised patients often raises suspicion regarding the potential for hospital acquisition. Hospital staff have an important responsibility in implementing preventive measures, especially since the advent of current legislation concerning hospital-acquired infections. There have been high expectations that molecular typing methods might determine the source of Aspergillus fumigatus, a ubiquitous mould. The aim of the present epidemiological study, was therefore, to identify the origin(s) of Aspergillus infection in six well-documented patients. All the clinical strains (N=33), and those from hospital (N=14) and home environments (N=34) were isolated according to a standardized protocol and typed by sequence-specific DNA primer analysis. The results confirmed the huge biodiversity of the A. fumigatus population, and consequently the difficulty in ascertaining a hospital source of the infection, as opposed to infections due to other Aspergillus species less frequently encountered.
authors.affiliation: Scientific Institute of Public Health-Louis Pasteur, Mycology Section, Brussels, Belgium. f.symoens@iph.fgov.be
authors.forename: F
authors.initials: F
authors.lastname: Symoens
authors.forename: J
authors.initials: J
authors.lastname: Burnod
authors.forename: B
authors.initials: B
authors.lastname: Lebeau
authors.forename: M A
authors.initials: MA
authors.lastname: Viviani
authors.forename: M A
authors.initials: MA
authors.lastname: Piens
authors.forename: A M
authors.initials: AM
authors.lastname: Tortorano
authors.forename: N
authors.initials: N
authors.lastname: Nolard
authors.forename: F
authors.initials: F
authors.lastname: Chapuis
authors.forename: R
authors.initials: R
authors.lastname: Grillot
mesh_terms: D000328:Adult; D000368:Aged; D001228:Aspergillosis / Q000453:epidemiology / Q000209:etiology* / Q000401:mortality; D001230:Aspergillus / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D003428:Cross Infection / Q000453:epidemiology / Q000209:etiology* / Q000401:mortality; D004781:Environmental Exposure; D005260:Female; D005602:France / Q000453:epidemiology; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008297:Male; D008875:Middle Aged