title: Immunocompetence of macrophages in rats exposed to Candida albicans infection and stress.
abstract: The integration of innate and adaptive immune responses is required for efficient control of Candida albicans. The present work aimed to assess, at the local site of the infection, the immunocompetence of macrophages in rats infected intraperitoneally with C. albicans and exposed simultaneously to stress during 3 days (CaS group). We studied the 1) ability to remove and kill C. albicans, 2) tumor necrosis factor-alpha (TNF-alpha) release, 3) balance of the inducible enzymes NO synthase (iNOS) and arginase, and 4) expression of interleukin (IL)-1beta and IL-1 receptor antagonist (ra) mRNA. Compared with only infected animals (Ca group), the number of colony-forming units was significantly higher in CaS rats (P < 0.01), and the macrophage candidicidal activity was approximately 2.5-fold lower (P < 0.01). Release of TNF-alpha was diminished in both unstimulated and heat-killed C. albicans restimulated macrophages of the CaS group (Ca vs. CaS, P < 0.03 and P < 0.05, respectively). In Ca- and CaS-group rats, the rates for both the arginase activity and the NO synthesis were significantly enhanced. However, the stress exposure downregulated the activity of both enzymes (CaS vs. Ca, P < 0.05). After in vitro restimulation, the IL-1ra/IL-1beta ratio was significantly diminished in CaS-group rats (P < 0.05). Our results indicate that a correlation exists between early impairment of macrophage function and stress exposure.
authors.affiliation: Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Pabellón Argentina 5000, Córdoba, Argentina.
authors.forename: Maria Cecilia
authors.initials: MC
authors.lastname: Rodriguez-Galán
authors.forename: Claudia
authors.initials: C
authors.lastname: Sotomayor
authors.forename: Maria Eugenia
authors.initials: ME
authors.lastname: Costamagna
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Cabanillas
authors.forename: Beatriz Salido
authors.initials: BS
authors.lastname: Rentería
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Masini-Repiso
authors.forename: Silvia
authors.initials: S
authors.lastname: Correa
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000254:growth & development / Q000276:immunology; D002177:Candidiasis / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D003345:Corticosterone / Q000097:blood; D003602:Cytotoxicity, Immunologic; D005260:Female; D007121:Immunocompetence / Q000502:physiology*; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism*; D010587:Phagocytosis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D003345:Corticosterone