Transcriptome analysis during cell division in plants.
Using synchronized tobacco Bright Yellow-2 cells and cDNA-amplified fragment length polymorphism-based genomewide expression analysis, we built a comprehensive collection of plant cell cycle-modulated genes. Approximately 1,340 periodically expressed genes were identified, including known cell cycle control genes as well as numerous unique candidate regulatory genes. A number of plant-specific genes were found to be cell cycle modulated. Other transcript tags were derived from unknown plant genes showing homology to cell cycle-regulatory genes of other organisms. Many of the genes encode novel or uncharacterized proteins, indicating that several processes underlying cell division are still largely unknown.
Breyne
Peter
P
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, K.L. Ledeganckstraat 35, Belgium.
Dreesen
Rozemarijn
R
Vandepoele
Klaas
K
De Veylder
Lieven
L
Van Breusegem
Frank
F
Callewaert
Lindy
L
Rombauts
Stephane
S
Raes
Jeroen
J
Cannoot
Bernard
B
Engler
Gilbert
G
Inzé
Dirk
D
Zabeau
Marc
M
D002453:Cell Cycle / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008938:Mitosis / Q000235:genetics; D011110:Polymorphism, Genetic; D014026:Nicotiana / Q000235:genetics*; D014158:Transcription, Genetic
D016207:Cytokines