title: Actions of gluco-oligosaccharide against Staphylococcus aureus.
abstract: Heavy colonization of atopic dermatitis (AD) with Staphylococcus aureus is well documented. The purpose of the present study is to examine the actions of gluco-oligosaccharide (G-OS) against S. aureus for the control of AD skin lesions infected with S. aureus. The colony counts of S. aureus cells in 0.5% sodium chloride solution supplemented with 5% G-OS (pH 4.8) were about 10-fold lower than those in 0.5% sodium chloride solution (pH 6.6; control) after incubation for 24 hours. The colony counts of S. aureus cells attached on the coverslips (pre-treatment with 1% and 5% G-OS/PBS and following treatment with plasma) were about 10-fold lower than those on the coverslips (pre-treatment with PBS and following treatment with plasma; control) in PBS after incubation for 24 hours. The materials (sugars, probably glycocalyx) that stained positively for fluorescein-isothiocyanate (FITC) -concanavalin A and were consistent with the presence of S. aureus cells were reduced when S. aureus cells attached to the coverslips treated with 5% GC-OS. In conclusion, C-OS is a promising agent that can be applied topically in a cream to clear adherent S. aureus cells from skin lesions of AD in order to prevent its exacerbation. Further, 5% C-OS can inhibit glycocalyx production by S. aureus cells and consequently have some suppressive effect on the colonization of S. aureus on the horny cells of AD lesions.
authors.affiliation: Department of Dermatology, Okayama University Graduate School of Medicine and Dentistry, Japan.
authors.forename: Hisanori
authors.initials: H
authors.lastname: Akiyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Oono
authors.forename: Wook Kang
authors.initials: WK
authors.lastname: Huh
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamasaki
authors.forename: Yukie
authors.initials: Y
authors.lastname: Akagi
authors.forename: Hideto
authors.initials: H
authors.lastname: Uemura
authors.forename: Tetsumasa
authors.initials: T
authors.lastname: Yamada
authors.forename: Keiji
authors.initials: K
authors.lastname: Iwatsuki
mesh_terms: D015169:Colony Count, Microbial; D003470:Culture Media; D003876:Dermatitis, Atopic / Q000188:drug therapy / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D009844:Oligosaccharides / Q000494:pharmacology*; D012016:Reference Values; D012680:Sensitivity and Specificity; D013207:Staphylococcal Skin Infections / Q000175:diagnosis / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D009844:Oligosaccharides