title: Biochemical engineering of the acyl side chain of sialic acids stimulates integrin-dependent adhesion of HL60 cells to fibronectin.
abstract: Sialylation of glycoproteins and glycolipids plays an important role during development, regeneration and pathogenesis of several diseases. The physiological precursor of all sialic acids is N-acetyl- D-mannosamine. The N-acyl side chain of sialic acid can be modified by exposure of cells to synthetic N-acyl-modified D-mannosamines. In a new experimental approach cells were cultivated in the presence of N-propanoyl- D-mannosamine. This unnatural precursor of sialic acid is taken up by cells and efficiently metabolized to the respective N-acyl-modified neuraminic acid in vitro and in vivo. Here we report on the biological consequences of the incorporation of the unnatural N-propanoylneuraminic acid into glycoconjugates of HL60 cells. Biochemical engineering of the acyl side chain of neuraminic acids activates beta(1)-integrins (VLA4 or VLA5), resulting in an increased adhesion of HL60 cells to fibronectin.
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, 14195 Berlin-Dahlem, Germany.
authors.forename: Pablo
authors.initials: P
authors.lastname: Villavicencio-Lorini
authors.forename: Stephan
authors.initials: S
authors.lastname: Laabs
authors.forename: Kerstin
authors.initials: K
authors.lastname: Danker
authors.forename: Werner
authors.initials: W
authors.lastname: Reutter
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Horstkorte
mesh_terms: D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D018929:Cell Culture Techniques; D005353:Fibronectins / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000494:pharmacology; D012794:Sialic Acids / Q000494:pharmacology*
chemical_list: D005353:Fibronectins; D006023:Glycoproteins; D019012:Integrin beta1; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid