title: [Food-grade gene expression systems for lactic acid bacteria].
abstract: Lactic acid bacteria (LAB) are important industrial microorganism used in the production and preservation of food-stuffs. Recently, considerable advances have been made in the genetics and molecular biology of LAB. These have resulted in the construction of food-grade gene expression systems for these bacteria. This paper aims to review the essential features for food-grade systems, food-grade selection markers, food-grade controlled gene expression and food-grade inducible signaling molecule, and recent developments on food-grade cloning and expression systems for LAB. These gene expression systems have great potential for studies on gene expression and regulation in LAB and a variety of bioprocessing application in industrial fermentations.
authors.affiliation: Research Center for Molecular Microbiology, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Zhang
authors.forename: Xiu-Zhu
authors.initials: XZ
authors.lastname: Chen
authors.forename: Shi-Fang
authors.initials: SF
authors.lastname: Jia
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Chen
authors.forename: Lian-Dong
authors.initials: LD
authors.lastname: Huan
mesh_terms: D019649:Food Industry / Q000379:methods / Q000592:standards; D005516:Food Microbiology; D015964:Gene Expression Regulation, Bacterial; D013294:Lactococcus lactis / Q000235:genetics* / Q000254:growth & development