title: Opening the high-pressure domain beyond 2 kbar to protein and virus crystallography--technical advance.
abstract: The combined use of a diamond anvil cell and ultrashort-wavelength undulator radiation has allowed the collection of high-resolution diffraction data from protein and virus crystals submitted to hydrostatic pressures beyond 2 kbar. Crystals of cubic cowpea mosaic virus (CPMV) can be compressed to at least 3.5 kbar. Diffraction from CPMV crystals displaying an unusual disorder at atmospheric pressure was considerably enhanced by application of pressure. These experiments suggest that pressure may be used in some cases to improve order in crystals.
authors.affiliation: SYNCHROTRON SOLEIL, bât. 209H, Université Paris-Sud, 91898 Orsay cedex, France. roger.fourme@soleil.u-psud.fr
authors.forename: Roger
authors.initials: R
authors.lastname: Fourme
authors.forename: Isabella
authors.initials: I
authors.lastname: Ascone
authors.forename: Richard
authors.initials: R
authors.lastname: Kahn
authors.forename: Mohamed
authors.initials: M
authors.lastname: Mezouar
authors.forename: Pierre
authors.initials: P
authors.lastname: Bouvier
authors.forename: Eric
authors.initials: E
authors.lastname: Girard
authors.forename: Tianwei
authors.initials: T
authors.lastname: Lin
authors.forename: John E
authors.initials: JE
authors.lastname: Johnson
mesh_terms: D003461:Crystallography / Q000379:methods*; D011312:Pressure; D011506:Proteins / Q000737:chemistry*; D014780:Viruses / Q000737:chemistry*
chemical_list: D011506:Proteins