Effective artificial proteases synthesized by covering silica gel with aldehyde and various other organic groups.
Organic artificial proteases with broad substrate specificity were synthesized by covering the surface of silica gel with aldehyde and the functional groups present in amino acids. The artificial proteases hydrolyzed ovalbumin, albumin, hemoglobin, gamma-globulin with half-lives as short as 50 min at 25 degrees C or 7 min at 50 degrees C.
Kim
Hyunsook
H
School of Chemistry and Center for Molecular Catalysis, Seoul National University, Republic of Korea.
Kim
Myoung-soon
MS
Paik
Hyesun
H
Chung
Yeon-Sook
YS
Hong
In Seok
IS
Suh
Junghun
J
D000447:Aldehydes / Q000737:chemistry*; D000818:Animals; D002384:Catalysis; D010450:Endopeptidases / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D018716:Molecular Mimicry; D009930:Organic Chemicals / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D058428:Silica Gel; D012822:Silicon Dioxide; D013379:Substrate Specificity
D000447:Aldehydes; D009930:Organic Chemicals; D011506:Proteins; D058428:Silica Gel; D012822:Silicon Dioxide; D010450:Endopeptidases