[Detection of P53 and K-ras gene mutations in lung cancer with oligonucleotide chip].
Different factors including hybridization solution components, hybridization temperature, and the concentration and proportion of the labelled primer, which affected the sensitivity and specificity of single mutation identification, were exploited. Asymmetric PCR increased the hybridization sensitivity, and the asymmetric multi-PCR did not affect the specificity, while the sensitivity was improved a little. Among 30 lung cancer samples detected with the oligonucleotide microarray, 12 was found P53 gene mutations and 5 had K-ras gene mutations. The P53 gene mutations identified by the oligonucleotide microarray was proved 80% same as the sequencing results. The obvious statistical relations of K-ras and P53 gene mutations with tumor type, tumor stage and smoking were not obtained because of less samples and mutation sites.
Liu
Jun-Bo
JB
ShenZhen Yishengtang Biopharmceutical Co., Ltd., Shenzhen 518260, China.
Xie
Ji-Pan
JP
Zou
Zong-Liang
ZL
Chen
Lin-Jie
LJ
Li
Long-Yun
LY
Wang
Sheng-Qi
SQ
D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009841:Oligonucleotides / Q000235:genetics; D012680:Sensitivity and Specificity; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D009841:Oligonucleotides; D016159:Tumor Suppressor Protein p53