title: Effect of cryopreservation on the structural and functional integrity of cell membranes of sugarcane (Saccharum sp.) embryogenic calluses.
abstract: In this paper, we investigated if the differences consistently noted in survival and plantlet production between cryopreserved and non-cryopreserved, control sugarcane embryogenic calluses were related to modifications induced during cryopreservation in the structural and functional integrity of cell membranes. For this, the evolution of electrolyte leakage, lipid peroxidation products and cell membrane protein contents was measured during 5 d after cryopreservation. Differences between control and frozen calluses were observed only during the first 2 (electrolyte leakage) or 3 d (lipid peroxidation products and membrane protein content) after freezing. It was not possible to link these differences with the differences noted in survival and plant production between control and cryopreserved calluses. Additional studies are thus needed to elucidate which biochemical factors, linked to survival and plantlet regeneration, are affected by cryopreservation.
authors.affiliation: Laboratorio de Mejoramiento Genético, Centro de Bioplantas, Universidad de Ciego de Avila, CP 69450, Ciego de Avila, Cuba. cubamarcose00@hotmail.com
authors.forename: M E
authors.initials: ME
authors.lastname: Martinez-Montero
authors.forename: N
authors.initials: N
authors.lastname: Mora
authors.forename: J
authors.initials: J
authors.lastname: Quiñones
authors.forename: M T
authors.initials: MT
authors.lastname: González-Arnao
authors.forename: F
authors.initials: F
authors.lastname: Engelmann
authors.forename: J C
authors.initials: JC
authors.lastname: Lorenzo
mesh_terms: D002462:Cell Membrane / Q000502:physiology*; D015925:Cryopreservation; D006801:Humans; D008315:Malondialdehyde / Q000032:analysis; D031786:Saccharum / Q000166:cytology* / Q000196:embryology
chemical_list: D008315:Malondialdehyde