A spectrophotometric assay for feruloyl esterases.
We have developed a spectrophotometric assay for the quantitative determination of feruloyl esterase activity based on release of 4-nitrophenol from a novel substrate, 4-nitrophenyl ferulate in an emulsion of Triton X-100 in aqueous buffer solution. The release of 4-nitrophenol was linear with reaction time at an early stage of the reaction with various esterase preparations. The method proposed here is accurate, rapid, and easy to perform.
Mastihuba
Vladimír
V
Faculty of Chemical Technology, Department of Milk, Fats, and Food Hygiene, Slovak University of Technology, Radlinského 9, Bratislava 812 37, Slovakia.
Kremnický
Lubomír
L
Mastihubová
Mária
M
Willett
J L
JL
Côté
Gregory L
GL
D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism; D004950:Esterases / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D009596:Nitrophenols / Q000032:analysis / Q000378:metabolism; D017830:Octoxynol / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013379:Substrate Specificity
D009596:Nitrophenols; D017830:Octoxynol; D004950:Esterases; D002265:Carboxylic Ester Hydrolases; D008049:Lipase; C070196:feruloyl esterase; D010450:Endopeptidases; C024836:4-nitrophenol