Schistosoma mansoni ferredoxin NADP(H) oxidoreductase and its role in detoxification.
Ferredoxin NADP(H) oxidoreductases (FNR) are flavoenzymes that catalyze the electron transfer between NADP(H) and a wide range of compounds including ferredoxins and bacterial flavodoxins. FNRs are classified into two major groups: plant- and vertebrate-type. Plant-type FNRs are implicated in photosynthesis and nitrogen fixation in plastids and photosynthetic bacteria, and were recently implicated in cell protection against reactive oxygen species (ROS). Vertebrate-type FNRs are mitochondrial enzymes implicated in steroid hormone biosynthesis in mammals and in Fe(+) uptake and metabolism in yeasts. We have cloned and sequenced a cDNA coding for the vertebrate-type Schistosoma mansoni FNR. Gel diaphorase activity and western blot assays demonstrated that SmFNR represented the major diaphorase activity of adult worms. An active recombinant SmFNR was expressed in Escherichia coli that made the bacteria tolerant to oxygen peroxide, cumene hydroperoxide and the superoxide-generating herbicide, methyl viologen (MV).
Girardini
Javier E
JE
Facultad de Ciencias Bioquimicas y Farmacéuticas, Instituto de Biologia Molecular y Celular de Rosario IBR-CONICET, UNR, Rosario, Argentina.
Dissous
Colette
C
Serra
Esteban
E
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D005260:Female; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D010545:Peroxides / Q000494:pharmacology; D012550:Schistosoma mansoni / Q000201:enzymology* / Q000254:growth & development; D017422:Sequence Analysis, DNA
D010545:Peroxides; D005287:Ferredoxin-NADP Reductase; D008058:Dihydrolipoamide Dehydrogenase; D010269:Paraquat