A novel single-stranded DNA expression vector.
A novel expression vector system has been developed that can intracellularly generate any single-stranded DNA (ssDNA) molecule, such as a triplex-forming oligonucleotide (TFO), antisense oligodeoxynucleotide (ODN) and DNA enzyme, by our laboratory at CytoGenix, Inc. 'Proof of concept' studies from our laboratory as well as our collaborators' indicate that this ssDNA expression vector system is capable of producing, intracellularly, antisense ODNs, DNA enzymes or TFOs for the purpose of downregulating gene expression or inducing targeted genome modification. This technology provides new research tools and has potential applications in gene target validation and drug development.
Chen
Yin
Y
CytoGenix, Inc., 9881 S. Wilcrest Drive, Houston, TX 77099, USA. ychen@cytogenix.com
D000818:Animals; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D019908:Proto-Oncogene Proteins c-raf / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
D004277:DNA, Single-Stranded; D019908:Proto-Oncogene Proteins c-raf; D001616:beta-Galactosidase