Antimicrobial peptides are expressed and produced in healthy and inflamed human synovial membranes.
The objective of this study was to determine the expression and production of antimicrobial peptides by healthy and inflamed human synovial membranes. Deposition of the antimicrobial peptides lysozyme, lactoferrin, secretory phospholipase A(2) (sPA(2)), matrilysin (MMP7), human neutrophil alpha-defensins 1-3 (HNP 1-3), human beta-defensin 1 (HBD-1), and human beta-defensin 2 (HBD-2) was determined by immunohistochemistry. Expression of mRNA for the antimicrobial peptides bactericidal permeability-increasing protein (BPI), heparin binding protein (CAP37), human cationic antimicrobial protein (LL37), human alpha-defensin 5 (HD5), human alpha-defensin 6 (HD6), HBD-1, HBD-2, and human beta-defensin 3 (HBD-3) was analysed by reverse transcription polymerase chain reaction (RT-PCR). RT-PCR revealed CAP37 and HBD-1 mRNA in samples of healthy synovial membrane. Additionally, HBD-3 and/or LL37 mRNA was detected in synovial membrane samples from patients with pyogenic arthritis (PA), osteoarthritis (OA) or rheumatoid arthritis (RA). BPI, HD5, HD6, and HBD-2 mRNAs were absent from all samples investigated. Immunohistochemistry identified lysozyme, lactoferrin, sPA(2), and MMP7 in type A synoviocytes of all samples. HBD-1 was only present in type B synoviocytes of some of the samples. Immunoreactive HBD-2 peptide was only visible in some inflamed samples. HNP1-3 was detected in both healthy and inflamed synovial membranes. The data suggest that human synovial membranes produce a broad spectrum of antimicrobial peptides. Under inflammatory conditions, the expression pattern changes, with induction of HBD-3 in PA (LL37 in RA; HBD-3 and LL37 in OA) as well as down-regulation of HBD-1. HBD-3 holds therapeutic potential in PA as it has a broad spectrum of antimicrobial activity and accelerates epithelial healing. However, caution is appropriate since defensins also promote fibrin formation and cell proliferation - key elements in joint infection. Clarification of the role of antimicrobial peptides in OA and RA will require further investigation.
Paulsen
Friedrich
F
Institute of Anatomy, Christian Albrecht University of Kiel, Germany. fpaulsen@anat.uni-kiel.de
Pufe
Thomas
T
Conradi
Lenard
L
Varoga
Deike
D
Tsokos
Michael
M
Papendieck
Jann
J
Petersen
Wolf
W
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001168:Arthritis / Q000378:metabolism*; D001170:Arthritis, Infectious / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D018929:Cell Culture Techniques; D023082:Defensins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007719:Knee Joint / Q000378:metabolism*; D020370:Osteoarthritis, Knee / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013583:Synovial Membrane / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D023082:Defensins