Cdc37 is essential for chromosome segregation and cytokinesis in higher eukaryotes.
Cdc37 has been shown to be required for the activity and stability of protein kinases that regulate different stages of cell cycle progression. However, little is known so far regarding interactions of Cdc37 with kinases that play a role in cell division. Here we show that the loss of function of Cdc37 in Drosophila leads to defects in mitosis and male meiosis, and that these phenotypes closely resemble those brought about by the inactivation of Aurora B. We provide evidence that Aurora B interacts with and requires the Cdc37/Hsp90 complex for its stability. We conclude that the Cdc37/Hsp90 complex modulates the function of Aurora B and that most of the phenotypes brought about by the loss of Cdc37 function can be explained by the inactivation of this kinase. These observations substantiate the role of Cdc37 as an upstream regulatory element of key cell cycle kinases.
Lange
Bodo M H
BM
European Molecular Biology Laboratory, Cell Biology and Biophysics Programme and Gene Expression Programme, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. Blange@EMBL-Heidelberg.de
Rebollo
Elena
E
Herold
Andrea
A
González
Cayetano
C
D000818:Animals; D064127:Aurora Kinases; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D020090:Chromosome Segregation; D004330:Drosophila / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D017344:Genes, Insect; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D008540:Meiosis; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D034622:RNA Interference; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism
C088085:Cdc37 protein, Drosophila; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D018841:HSP90 Heat-Shock Proteins; D018832:Molecular Chaperones; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases