title: Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation.
abstract: A large number of hDAF transgenic pigs to be used for xenotransplantation research were generated by using sperm-mediated gene transfer (SMGT). The efficiency of transgenesis obtained with SMGT was much greater than with any other method. In the experiments reported, up to 80% of pigs had the transgene integrated into the genome. Most of the pigs carrying the hDAF gene transcribed it in a stable manner (64%). The great majority of pigs that transcribed the gene expressed the protein (83%). The hDAF gene was transmitted to progeny. Expression was stable and found in caveolae as it is in human cells. The expressed gene was functional based on in vitro experiments performed on peripheral blood mononuclear cells. These results show that our SMGT approach to transgenesis provides an efficient procedure for studies involving large animal models.
authors.affiliation: Dipartimento di Medicina Sperimentale, Università La Sapienza, 00161 Roma, Italy. marialuisa.lavitrano@unibo.it
authors.forename: Marialuisa
authors.initials: M
authors.lastname: Lavitrano
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Bacci
authors.forename: Monica
authors.initials: M
authors.lastname: Forni
authors.forename: Davide
authors.initials: D
authors.lastname: Lazzereschi
authors.forename: Carla
authors.initials: C
authors.lastname: Di Stefano
authors.forename: Daniela
authors.initials: D
authors.lastname: Fioretti
authors.forename: Paola
authors.initials: P
authors.lastname: Giancotti
authors.forename: Gabriella
authors.initials: G
authors.lastname: Marfé
authors.forename: Loredana
authors.initials: L
authors.lastname: Pucci
authors.forename: Luigina
authors.initials: L
authors.lastname: Renzi
authors.forename: Hongjun
authors.initials: H
authors.lastname: Wang
authors.forename: Antonella
authors.initials: A
authors.lastname: Stoppacciaro
authors.forename: Giorgio
authors.initials: G
authors.lastname: Stassi
authors.forename: Massimo
authors.initials: M
authors.lastname: Sargiacomo
authors.forename: Paola
authors.initials: P
authors.lastname: Sinibaldi
authors.forename: Valeria
authors.initials: V
authors.lastname: Turchi
authors.forename: Roberto
authors.initials: R
authors.lastname: Giovannoni
authors.forename: Giacinto
authors.initials: G
authors.lastname: Della Casa
authors.forename: Eraldo
authors.initials: E
authors.lastname: Seren
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Rossi
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D018958:CD55 Antigens / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D018014:Gene Transfer Techniques; D006801:Humans; D008297:Male; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine; D019076:Transgenes; D014183:Transplantation, Heterologous
chemical_list: D018958:CD55 Antigens; D004247:DNA