An in vitro screening paradigm for extracts of whole foods for detection of potential toxicants.
The application of organic, conventional and biotechnology techniques can alter the intrinsic levels of natural toxicants in crop foods and methods are needed to screen for unexpected changes in toxicant levels. We evaluated crude, aqueous preparations of 37 foods purchased from a local market in a battery of four in vitro mammalian toxicity screens. The foods were evaluated in one or more of the following tests: (1) cytotoxicity (37 foods) and (2) chromosomal aberration test (nine foods), both in Chinese hamster ovary cells, (3) limb bud micromass assay (nine foods) using 11-day old CD-1 mouse embryos and (4) estrogenicity (MCF-7 cells transfected with estrogen receptor and lucerifase reporter constructs, 12 foods). IC50s for cellular proliferation ranged from < 1% (v/v, garlic) to > 10% (v/v, 18 foods), the maximal concentration tested. Five of nine preparations (soybeans, broccoli, garlic, snow peas and corn) were clastogenic and two (soybeans and snow peas) inhibited chrondrogenesis in the limb bud micromass assay. Five of nine preparations (soybeans, snow peas, cumin, asparagus and bean sprouts) produced significant estrogenic responses. Overall, the 12 foods evaluated in two or more of the tests showed different patterns of response. These preliminary data indicate that screening for potential toxicants is possible with fast, relatively inexpensive in vitro tests. These in vitro tests, while potentially useful to detect unexpected toxicants in plants that may signal the need for further evaluation, are not directly useful to predict human or animal risk from eating these plants.
Charles
G D
GD
gdcharles@dow.com
Linscombe
V A
VA
Tornesi
B
B
Mattsson
J L
JL
Gollapudi
B B
BB
D000014:Abnormalities, Drug-Induced; D000818:Animals; D001943:Breast Neoplasms; D016466:CHO Cells; D016923:Cell Death / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D004622:Embryo, Mammalian; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D005638:Fruit / Q000737:chemistry; D051379:Mice; D009152:Mutagenicity Tests; D011247:Pregnancy; D011960:Receptors, Estrogen; D013025:Glycine max / Q000737:chemistry; D013723:Teratogens / Q000032:analysis; D018675:Toxicity Tests; D014407:Tumor Cells, Cultured; D014675:Vegetables / Q000737:chemistry
D004967:Estrogens; D011960:Receptors, Estrogen; D013723:Teratogens