Cyclohexylamino-demethoxy-hypocrellin B and photodynamic therapy decreases human cancer in vitro.
2-Cyclohexylamino-2-demethoxy-hypocrellin B (CHAHB) is a new photosensitizer synthesized by the mild reaction between hypocrellin B (HB) and cyclohexylamine, in which the peri-hydroxylated perylenequinone structure of the parent HB is preserved and the photoresponse is enhanced markedly. Electron paramagnetic resonance (EPR) spin trapping measurements and 9,10-diphenylanthracene (DPA) bleaching studies were employed to investigate the photodynamic action of CHAHB in the presence of oxygen. Singlet oxygen ((1)O2) and superoxide anion radical (O2*-) generated by illuminating CHAHB in aerobic solution have been observed. Compared with HB, CHAHB distinctly enhanced the O2(*-)-generating abilities, although (1)O2-generating abilities were lowered. The photodynamic action of CHAHB in the therapy of cancer was investigated in vitro. The results in vitro revealed a much more significant decrease in cancer cell growth than with HB. Laser or dye alone had no effect, indicating that intratumor CHAHB and laser therapy may prove useful in unresectable cancer.
Xu
S
S
Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, PR China.
Chen
S
S
Zhang
M
M
Shen
T
T
Liu
Z
Z
Zhao
Y
Y
Wu
Y
Y
D002460:Cell Line / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D003497:Cyclic N-Oxides / Q000138:chemical synthesis / Q000627:therapeutic use; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010190:Pancreatic Neoplasms / Q000188:drug therapy*; D010569:Perylene / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000627:therapeutic use*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000627:therapeutic use*; D011809:Quinones / Q000138:chemical synthesis / Q000627:therapeutic use*; D017382:Reactive Oxygen Species; D014407:Tumor Cells, Cultured / Q000187:drug effects
C467707:2-cyclohexylamino-2-demethoxyhypocrellin B; D003497:Cyclic N-Oxides; D005609:Free Radicals; D017319:Photosensitizing Agents; D011809:Quinones; D017382:Reactive Oxygen Species; D010569:Perylene