Cloning and identification of a novel cDNA which may be associated with FKBP25.
A 1209 bp novel cDNA with a putative open reading frame of 627 bp that has an overlapped fragment of 527 bp with FKBP25 was isolated from a human fetal brain library. Northern Blot analysis detected a signal about 1.5 kb that suggests it is a full-length cDNA. Reverse transcription-PCR demonstrates that the transcripts are high in adult brain, testis, ovary, spleen, and fetal brain. Comparing the cDNA with the human genome database identifies a contig that reveals that the gene contains six exons and is located on human chromosome 20p11. The gene encodes a putative protein with an unknown domain conserved in many species and is similar to bacterial histidyl-tRNA synthetase, and so it is named human histidyl-tRNA-synthetase-related gene.
Meng
Xianfang
X
State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, People's Republic of China.
Chen
Jinzhong
J
Yang
Quansheng
Q
Wang
Shu
S
Chao
Yaoqiong
Y
Ying
Kang
K
Xie
Yi
Y
Mao
Yumin
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002890:Chromosomes, Human, Pair 20; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D006643:Histidine-tRNA Ligase / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D022021:Tacrolimus Binding Proteins / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; C074856:FKBP3 protein, human; D022021:Tacrolimus Binding Proteins; D006643:Histidine-tRNA Ligase