Whole inactivated SIV virion vaccines with functional envelope glycoproteins: safety, immunogenicity, and activity against intrarectal challenge.
A novel type of whole inactivated simian immunodeficiency virus (SIV) virion vaccine immunogen with functional envelope glycoproteins was evaluated, without adjuvant, in rhesus macaques. Immunogens included purified inactivated virions of SIVmac239, a designed mutant of SIVmac239 with gp120 carbohydrate attachment sites deleted (SIVmac239 g4,5), and SIVmneE11S. The vaccines were noninfectious, safe, and immunogenic, inducing antibody responses and cellular responses, including responses by CD8+ lymphocytes. Interpretation of protective efficacy following intrarectal challenge was complicated by incomplete take of the challenge in some SIV naïve controls.
Lifson
J D
JD
AIDS Vaccine Program, SAIC Frederick, National Cancer Institute at Frederick, MD 21702, USA. lifson@avpaxp1.ncifcrf.gov
Piatak
M
M
Rossio
J L
JL
Bess
J
J
Chertova
E
E
Schneider
D
D
Kiser
R
R
Coalter
V
V
Poore
B
B
Imming
R
R
Desrosiers
R C
RC
Henderson
L E
LE
Arthur
L O
LO
D000285:Administration, Rectal; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015686:Gene Products, env / Q000276:immunology*; D008213:Lymphocyte Activation; D008253:Macaca mulatta / Q000276:immunology* / Q000821:virology*; D008297:Male; D012367:RNA, Viral / Q000097:blood; D018449:SAIDS Vaccines / Q000009:adverse effects* / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000502:physiology; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000009:adverse effects* / Q000276:immunology*; D019562:Viral Load
D000914:Antibodies, Viral; D015686:Gene Products, env; D012367:RNA, Viral; D018449:SAIDS Vaccines; D014613:Vaccines, Attenuated