title: The application of the AFLP method to determine the purity of homozygous lines of barley (Horedum vulgare L.).
abstract: Amplified fragment length polymorphism of DNA has been used to analyse the equality of plants obtained from isolated microspores. Although the control parental material was regarded as being highly homozygous, the analysis of the banding patterns of single plants showed a certain level of polymorphism. The analysis of regenerants with a doubled chromosome number did not show any diversity within the progeny of a single line. The differences in banding patterns coming from single plants were only observed in microspore donor lines. These results have proven the high purity of homozygous lines obtained via androgenesis from isolated microspores.
authors.affiliation: Department of Biotechnology and Plant Cytogenetics, Plant Breeding and Acclimatization Institute, Radzików, 05-870 Błonie, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Oleszczuk
authors.forename: Janusz
authors.initials: J
authors.lastname: Zimny
authors.forename: Piotr Tomasz
authors.initials: PT
authors.lastname: Bednarek
mesh_terms: D001947:Breeding; D018744:DNA, Plant / Q000235:genetics; D006720:Homozygote; D001467:Hordeum / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011123:Polyploidy; D012038:Regeneration
chemical_list: D018744:DNA, Plant