title: Trypsin-catalysed synthesis of oligopeptide amides: comparison of catalytic efficiency among trypsins of different origin (bovine, Streptomyces griseus and chum salmon).
abstract: A procedure has been developed for the synthesis of oligopeptide amide using inverse substrates as acyl donors with amino acid amide instead of p-nitroanilide as acyl acceptor and trypsins of different origin (bovine, Streptomyces griseus and chum salmon trypsins) as the catalyst. The effectiveness of this procedure was demonstrated by the synthesis of a pentapeptide, Boc-[Leu5]-enkephalin amide, as a model compound. The method was the first enzymatic method shown to be successful at each successive coupling step for the synthesis of the oligopeptide. Bovine and chum salmon trypsins were superior to Streptomyces griseus trypsin as the catalyst.
authors.affiliation: Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Japan.
authors.forename: Haruo
authors.initials: H
authors.lastname: Sekizaki
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Itoh
authors.forename: Eiko
authors.initials: E
authors.lastname: Toyota
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Tanizawa
mesh_terms: D000577:Amides / Q000138:chemical synthesis*; D000818:Animals; D002417:Cattle; D009842:Oligopeptides / Q000138:chemical synthesis*; D017685:Oncorhynchus keta / Q000378:metabolism; D013305:Streptomyces griseus / Q000201:enzymology; D014357:Trypsin / Q000378:metabolism*
chemical_list: D000577:Amides; D009842:Oligopeptides; D014357:Trypsin