Ni+-affinity purification of untagged cAMP receptor protein.
Wickstrum
Jason R
JR
University of Kansas, Lawrence, USA.
Egan
Susan M
SM
D002846:Chromatography, Affinity / Q000379:methods; D002373:Cyclic AMP Receptor Protein / Q000737:chemistry / Q000302:isolation & purification*; D004926:Escherichia coli / Q000378:metabolism; D006639:Histidine / Q000737:chemistry; D007475:Ion Exchange Resins; D009532:Nickel / Q000737:chemistry; D011137:Polystyrenes; D011145:Polyvinyls; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification
D002373:Cyclic AMP Receptor Protein; D007475:Ion Exchange Resins; D011137:Polystyrenes; D011145:Polyvinyls; D011993:Recombinant Fusion Proteins; D006639:Histidine; D009532:Nickel; C006960:chelex