title: An electron microscopic study of neuronal degeneration and glial cell reaction in the retina of glaucomatous rats.
abstract: The present investigation was focused on the ultrastructural changes in the neurons and glial cells in the retina of rats with experimentally-induced glaucoma. An experimental glaucoma model was created by limbal-derived vein cauterization. Animals were sacrificed at 1, 3 weeks and 3 months post-operation. Retinae were dissected and processed for electron microscopy. Neuronal degeneration was observed in all the different layers of the retina at both 1 and 3 weeks post-operation. Some degenerating neurons were found in the ganglion cell layer (GCL), inner nuclear layer (INL) and outer nuclear layer (ONL). And the dying neurons presented apoptotic-like more than necrotic neurons. Many degenerating axons and axon terminals were observed between neurons in the GCL, inner plexiform layer (IPL), INL, and outer plexiform layer (OPL). Activated astrocytes and microglial cells were present in close association with degenerating neurons and axons. The Müller cells in the INL also presented longer and darker processes with more microfilaments than in normal cells. Degenerating neuronal debris, degenerating axonal profiles and electron-dense bodies were often found in the cytoplasm of macrophages. The results suggest that both microglial cells and astrocytes are activated in the process of neuronal degeneration in the retina of experimentally-induced glaucomatous rats. It is hypothesized that they may play a protective role in removing degenerating neuronal elements in the retina after the onset of glaucoma.
authors.affiliation: Department of Anatomy, Faculty of Medicine, National University of Singapore, MD10, 4 Medical Drive, Singapore 117597.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: S S W
authors.initials: SS
authors.lastname: Tay
authors.forename: Y K
authors.initials: YK
authors.lastname: Ng
mesh_terms: D000818:Animals; D016923:Cell Death; D005901:Glaucoma / Q000473:pathology* / Q000503:physiopathology; D020287:In Situ Nick-End Labeling; D007429:Intraocular Pressure / Q000502:physiology; D008297:Male; D008854:Microscopy, Electron; D009410:Nerve Degeneration / Q000473:pathology*; D009457:Neuroglia / Q000473:pathology* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000473:pathology* / Q000648:ultrastructure; D016707:Tissue Fixation