BanAI a new isoschizomer of the type II restriction endonuclease HaeIII discovered in a Bacillus anthracis isolate from Amazon Basin.
Bacillus anthracis was isolated and identified from a bacterial collection of samples from the Amazon river bank. Type II restriction endonuclease activity was detected in this prokaryote, the enzyme was purified, the molecular mass of the native protein estimated by gel filtration, and optima pH, temperature and salt requirements were determined. Quality control assays showed complete absence of 'non-specific nucleases'. Restriction cleavage analysis and DNA sequencing of restriction fragments allowed unequivocal demonstration of 5'-GG downward arrow CC-3' as the recognition sequence. This enzyme was named BanAI and is therefore an isoschizomer of the prototype restriction endonuclease HaeIII. This is the first report of a type II restriction endonuclease identified, purified from a natural isolate of B. anthracis.
Chies
Jocelei M
JM
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. jocelei@dna.cbiot.ufrgs.br
de O  Dias
Ana C
AC
Maia
Helio M M
HM
Astolfi-Filho
Spartaco
S
D001408:Bacillus anthracis / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D001938:Brazil; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics* / Q000302:isolation & purification*
D001426:Bacterial Proteins; C031851:endodeoxyribonuclease HaeII; D015252:Deoxyribonucleases, Type II Site-Specific