title: groEL expression in gyrB mutants of Borrelia burgdorferi.
abstract: GroEL protein and groEL mRNA transcript were up-regulated in gyrB mutants of Borrelia burgdorferi, a causative agent of Lyme disease. Furthermore, the protein and transcript levels in gyrB mutants were greater than those in experimentally heat-shocked cultures of wild-type B. burgdorferi. Circular DNA in the gyrB mutants was more relaxed than in wild-type cells, although groEL is on the linear chromosome of B. burgdorferi. To our knowledge, this is the first evidence, albeit indirect, for the effect of DNA topology on gene expression from a linear DNA molecule in a bacterium.
authors.affiliation: Division of Biological Sciences, The University of Montana, Missoula, Montana 59812, USA. janalverson@msa-msstate.ars.usda.gov
authors.forename: Janet
authors.initials: J
authors.lastname: Alverson
authors.forename: D Scott
authors.initials: DS
authors.lastname: Samuels
mesh_terms: D049933:Aminocoumarins; D025065:Borrelia burgdorferi / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D018834:Chaperonin 60 / Q000235:genetics* / Q000378:metabolism; D003374:Coumarins / Q000494:pharmacology; D027081:DNA Gyrase / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D009690:Nucleic Acid Conformation; D059005:Topoisomerase II Inhibitors; D015854:Up-Regulation
chemical_list: D049933:Aminocoumarins; D018834:Chaperonin 60; D003374:Coumarins; D059005:Topoisomerase II Inhibitors; D027081:DNA Gyrase; C004628:coumermycin