Determination of nitroglycerin and its dinitrate metabolites in urine by gas chromatography-mass spectrometry as potential biomarkers for occupational exposure.
This paper describes a method for the quantitative analysis of nitroglycerin and its dinitrate metabolites (1,2- and 1,3-glycerol dinitrate) in urine. After liquid-liquid extraction the analytes were separated and quantified using gas chromatography-mass spectrometry with negative ion chemical ionisation. The method can detect above 0.3 nmol/l for all analytes and is linear over the range of at least 0-44 nmol/l. The method was then used to determine metabolite levels in a subject using nitroglycerin therapeutically for the treatment of angina. Metabolites were stable in urine for at least 6 days at room temperature, approximately 4 degrees C and -20 degrees C.
Akrill
Peter
P
Health and Safety Laboratory, Broad Lane, Sheffield S3 7HQ, UK.
Cocker
John
J
D015415:Biomarkers / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D009566:Nitrates / Q000652:urine*; D005996:Nitroglycerin / Q000652:urine*; D016273:Occupational Exposure; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015415:Biomarkers; D009566:Nitrates; D005996:Nitroglycerin