Characterisation of tumour-associated antigens in colon cancer.
In order to search for clinically relevant cancer-associated genes and to define further the spectrum of immunogenic proteins, we applied SEREX (serological identification of antigens by recombinant expression cloning) to analyse genes expressed in colon adenocarcinoma. Eight different serum-reactive cDNA clones were isolated by immunoscreening from a colon cancer-derived cDNA expression library. mRNA expression studies showed that 2 of them, RHAMM and AD034, have a differential tissue distribution, and that 3 genes, NAP1L1, RHAMM and AD034, are overexpressed in tumours in comparison with the adjacent non-cancerous tissues. 5' RLM-RACE analysis of AD034, a sequence with a tyrosine kinase motif, revealed a frameshifting insertion of 32 bp, most likely generated by use of cryptic splice site in tumour-derived cDNA. Analysis of full-length RHAMM cDNA sequence revealed the presence of two splice variants, which are known to have a different sub-cellular localisation; expression of these splice variants is altered in colon cancer tissues. Serological responses to three antigens (C21ORF2, EPRS and NAP1L1) were found mainly in cancer patients' sera.
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Aija
A
Biomedical Research and Study Centre, University of Latvia, 1 Ratsupites St, LV-1067, Riga, Latvia. aija@biomed.lu.lv
Slucka
Zane
Z
Stengrevics
Aivars
A
Silina
Karina
K
Li
Geng
G
Rees
Robert C
RC
D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology*; D017398:Alternative Splicing; D000912:Antibodies, Neoplasm / Q000097:blood / Q000276:immunology; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D003110:Colonic Neoplasms / Q000235:genetics / Q000276:immunology*; D016326:Extracellular Matrix Proteins / Q000235:genetics; D015723:Gene Library; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics; D007518:Isoantibodies / Q000276:immunology; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger / Q000096:biosynthesis; D014018:Tissue Distribution
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D016326:Extracellular Matrix Proteins; D018960:Hyaluronan Receptors; D007518:Isoantibodies; D012333:RNA, Messenger; C098946:hyaluronan-mediated motility receptor; D017346:Protein Serine-Threonine Kinases; C469385:RIOK1 protein, human