Monitoring of apoptosis of HL60 cells by Fourier-transform infrared spectroscopy.
Fourier-transform infrared (FTIR) spectroscopy is a vibrational technique that gives information on the chemical composition of a sample, providing a "molecular fingerprint" of it. It is a powerful approach to study intact cells. The aim of the present study was to analyse and quantify apoptotic cells by using a FTIR approach based on attenuated total reflection (ATR). We incubated human HL60 leukaemic cells with camptothecin, a cytotoxic drug, and monitored apoptosis induction over a period of time. Several ATR-FTIR spectral changes occurred during the apoptotic process. In particular, we observed that the apoptotic index was inversely correlated with the spectral area in the region 1200-900 cm(-1), assigned to the absorption of nucleic acids. We therefore propose that ATR-FTIR spectral features may be used as a diagnostic marker of apoptotic cells.
Gasparri
Fabio
F
DRO-Oncology, Pharmacology Department, Pharmacia Corp., Via Pasteur 10, 20014 Nerviano, Italy.
Muzio
Marta
M
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis; D002166:Camptothecin / Q000494:pharmacology; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D048429:Cell Size; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared
D000590:Amino Acid Chloromethyl Ketones; D000972:Antineoplastic Agents, Phytogenic; D061945:Caspase Inhibitors; D015853:Cysteine Proteinase Inhibitors; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; D004247:DNA; D020169:Caspases; D002166:Camptothecin