Modulation of neuritogenesis by ganglioside-specific sialidase (Neu 3) in human neuroblastoma NB-1 cells.
Plasma membrane-associated sialidase (Neu 3), which specifically hydrolyzes gangliosides, is relatively abundantly present in the nervous system. To understand the role of Neu 3 in neuronal differentiation, we studied the relationship between neurite outgrowth and Neu 3 expression in human neuroblastoma NB-1 cells. The expression of Neu 3 in NB-1 cells increased when neurite outgrowth in these cells was induced by dibutyryl cAMP. While treatment with dibutyryl cAMP alone enhanced the outgrowth of dendrite-like processes, transfection of the Neu 3 gave rise to a more prominent outgrowth of neurites with axon-like characteristics, even in the absence of dibutyryl cAMP. Neu 3 induction by dibutyryl cAMP is probably attributable, in part, to transactivation of the Neu 3 gene through cAMP responsive elements in the 5'-upstream region, as revealed by the promotor activity assay using Neu 3 promotor expression plasmid. These results indicate that Neu 3 regulates neurite formation in NB-1 cells, and suggest that this effect may be enhanced by dibutyryl cAMP via a cAMP-dependent pathway.
Proshin
Sergey
S
Division of Biochemistry, Research Institute of Miyagi Prefectural Cancer Center, Natori, Japan.
Yamaguchi
Kazunori
K
Wada
Tadashi
T
Miyagi
Taeko
T
D001483:Base Sequence; D003994:Bucladesine / Q000494:pharmacology; D017931:DNA Primers; D006801:Humans; D009439:Neuraminidase / Q000235:genetics / Q000378:metabolism*; D016501:Neurites; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D003994:Bucladesine; C465436:Neu3 protein, human; D009439:Neuraminidase