Embryonic brain expression analysis of lysophospholipid receptor genes suggests roles for s1p(1) in neurogenesis and s1p(1-3) in angiogenesis.
In a comparison of embryonic brain expression patterns of lysophosphatidic acid and sphingosine 1-phosphate receptor genes (lpa(1-3) and s1p(1-5), respectively), transcripts detected by Northern blot were subsequently localized using in situ hybridization. We found striking s1p(1) expression adjacent to several ventricles. Near the lateral ventricle, s1p(1) expression was temporally and spatially coincident with neurogenesis and overlapped with lpa(1) in the neocortical area. We also observed a widespread diffuse pattern for lpa(2-3) and a scattered punctate pattern for s1p(1-3). The punctate pattern colocalized with vascular endothelial markers. Together, these results suggest that s1p(1) influences neurogenesis and s1p(1-3) influence angiogenesis in the developing brain.
McGiffert
Christine
C
Neurosciences Graduate Program, School of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0636, USA.
Contos
James J A
JJ
Friedman
Beth
B
Chun
Jerold
J
D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division; D002540:Cerebral Cortex / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D017403:In Situ Hybridization; D008246:Lysophospholipids / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic; D018919:Neovascularization, Physiologic; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid; D049312:Receptors, Lysophospholipid; D013997:Time Factors; D014018:Tissue Distribution
D018952:Antigens, CD34; D008246:Lysophospholipids; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid; D049312:Receptors, Lysophospholipid; D001973:Bromodeoxyuridine