A novel brain-expressed protein related to carnitine palmitoyltransferase I.
Malonyl-CoenzymeA acts as a fuel sensor, being both an intermediate of fatty acid synthesis and an inhibitor of the two known isoforms of carnitine palmitoyltransferase I (CPT I), which control mitochondrial fatty acid oxidation. We describe here a novel CPT1 family member whose mRNA is present predominantly in brain and testis. Chromosomal locations and genome organization are reported for the mouse and human genes. The protein sequence contains all the residues known to be important for both carnitine acyltransferase activity and malonyl-CoA binding in other family members. Yeast expressed protein has no detectable catalytic activity with several different acyl-CoA esters that are good substrates for other carnitine acyltransferases, including the liver isoform of CPT I, which is also expressed in brain; however, it displays high-affinity malonyl-CoA binding. Thus this new CPT I related protein may be specialized for the metabolism of a distinct class of fatty acids involved in brain function.
Price
Nigel
N
Hannah Research Institute, Ayr, KA6 5HL, UK. pricen@hri.sari.ac.uk
van der Leij
Feike
F
Jackson
Vicky
V
Corstorphine
Clark
C
Thomson
Ross
R
Sorensen
Annette
A
Zammit
Victor
V
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000276:immunology / Q000378:metabolism*; D002334:Carnitine O-Palmitoyltransferase / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D002874:Chromosome Mapping; D017124:Conserved Sequence; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D016415:Sequence Alignment
D018076:DNA, Complementary; D002334:Carnitine O-Palmitoyltransferase