Biological monitoring of workers exposed to dichloromethane, using head-space gas chromatography.
A biological monitoring method for urinary dichloromethane (DCM) has been developed by using head-space gas chromatography with FID detection. The calibration curve is linear in a wide range of DCM levels between 0.01 and 2 mg/l. The recovery rate is almost 100% and within-run coefficients of variation are 2.9-3.7%. A highly significant correlation is found between exposure levels and urinary concentrations of DCM. Determination of urine DCM by this method has many advantages such as sample storage, no need for correction of urine concentration, absence of gender difference and also no confounding effect of glutathione S-transferase T1 polymorphism.
Sakai
Tadashi
T
Occupational Poisoning Center, Tokyo Rosai Hospital, 13-21 Omoriminami-4, Ota-ku, Tokyo 143-0013, Japan. opc@msa.biglobe.ne.jp
Morita
Yoko
Y
Wakui
Chuji
C
D002849:Chromatography, Gas / Q000379:methods*; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D008752:Methylene Chloride / Q000652:urine*; D016273:Occupational Exposure; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results
D005982:Glutathione Transferase; D008752:Methylene Chloride; C413545:glutathione S-transferase T1