[Anther culture generated stem borer-resistance DH lines of Minghui 81(Oryza sativa L. subsp. indica) expressing modified cry1Ac gene].
2600 Anthers from T0 modified cry1 Ac-transgenic rice lines of Minghui 81, an elite restoring line of commercial CMS indica hybrid rice, were cultured on SK3 media. 83 green plantlets were recovered, 43 double haploid (DH) and 40 haploid among them. Results of PCR analyzes indicated that 55 plants of 83 were harbored the cry1Ac gene, and the ratio of cry1Ac-positive against cry1Ac-negative was 2:1 (55/28). 36 putative transgenic DH plants were further confirmed by Southern blot. ELISA detection showed that Cry1Ac level in different transgenic rice plants of the same cry1Ac-DH clone was almost equal and the highest one amount to 0.25% of the total soluble protein. Pest insect-resistant bioassay at field trials demonstrated that some of the homozygous cry1Ac-transgenic rice plants not only showed high-level resistance against striped stem borer (Chilo suppressalis) but also retained elite agronomy characters. These results demonstrated that rice anther culture has a great value in rice molecular breeding.
Zeng
Qian-Chun
QC
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Wu
Qian
Q
Feng
De-Jiang
DJ
Zhou
Kai-Da
KD
Liu
Xiang
X
Zhu
Zhen
Z
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001427:Bacterial Toxins; D015139:Blotting, Southern; D046508:Culture Techniques; D018744:DNA, Plant / Q000235:genetics; D004731:Endotoxins / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D006460:Hemolysin Proteins; D007113:Immunity, Innate / Q000235:genetics; D009036:Moths / Q000254:growth & development*; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000469:parasitology; D018514:Plant Structures / Q000235:genetics / Q000254:growth & development* / Q000469:parasitology; D030821:Plants, Genetically Modified
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D018744:DNA, Plant; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis