Microfabricated 384-lane capillary array electrophoresis bioanalyzer for ultrahigh-throughput genetic analysis.
A microfabricated 384-lane capillary array electrophoresis device is developed and utilized for massively parallel genetic analysis. The 384 capillary lanes, arrayed radially about the center of a 200-mm-diameter glass substrate sandwich, are constructed using scalable microfabrication techniques derived from the semiconductor industry. Samples are loaded into reservoirs on the perimeter of the wafer, separated on the 8-cm-long poly(dimethylacrylamide) gel-filled channels, and detected with a four-color rotary confocal fluorescence scanner. The performance and throughput of this bioanalyzer are demonstrated by simultaneous genotyping 384 individuals for the common hemochromatosis-linked H63D mutation in the human HFE gene in only 325 s. This lab-on-a-chip device thoroughly exploits the power of microfabrication to produce high-density capillary electrophoresis arrays and to use them for high-throughput bioanalysis.
Emrich
Charles A
CA
Department of Chemistry, University of California, Berkeley 94720, USA.
Tian
Huijun
H
Medintz
Igor L
IL
Mathies
Richard A
RA
D004247:DNA / Q000737:chemistry / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D005838:Genotype; D006801:Humans; D008838:Microcomputers; D008967:Molecular Biology / Q000295:instrumentation*; D036103:Nanotechnology
D004247:DNA