Effects of phthalate esters on actin cytoskeleton of Py1a rat osteoblasts.
We evaluated, by confocal laser scanning microscopy, the actin cytoskeleton of immortalized rat Py1a osteoblasts treated with phthalate esters (butyl benzyl phthalate, BBP and dibutyl phthalate, DBP), endocrine disruptors with estrogenic activity. We observed some peculiar modifications of actin cytoskeleton and cells changing from a spindle shape to a rounded form. In particular, F-actin formed thick bundles around the cell membrane but only a weak labeling was observed in rounded cells. Also influence on apoptosis and short-term effects on FGF-2 were studied. It was found that BBP and DBP exert their action in a similar way, act in a transient manner and do not induce apoptosis.
Marchetti
L
L
Department of Comparative Morphology and Biochemistry, University of Camerino, Italy.
Sabbieti
M G
MG
Menghi
M
M
Materazzi
S
S
Hurley
M M
MM
Menghi
G
G
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism*; D053938:DNA Fragmentation; D003993:Dibutyl Phthalate / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D019793:Fluorescein; D018613:Microscopy, Confocal; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D010795:Phthalic Acids / Q000494:pharmacology*; D051381:Rats; D013723:Teratogens / Q000494:pharmacology*
D000199:Actins; D010795:Phthalic Acids; D013723:Teratogens; D016222:Fibroblast Growth Factor 2; D003993:Dibutyl Phthalate; D019793:Fluorescein; C027561:butylbenzyl phthalate