title: Characterization of Clostridium butyricum neurotoxin associated with food-borne botulism.
abstract: The neurotoxin of Clostridium butyricum strain LCL155 (BuNT/LCL155) associated with type E food-borne botulism showed antigenic and biological properties different from those of C. botulinum type E (BoNT/E) andC. butyricum strain BL5262 (BuNT/BL5262). The specific toxicity of BuNT/LCL155 was found to be about 10% those of BoNT/E and BuNT/BL5262. Immunological analysis with monoclonal antibodies against BoNT/E showed that the heavy chain of BuNT/LCL155 differs partially from those of BoNT/E and BuNT/BL5262. Binding experiments with rat brain synaptic membrane revealed that BuNT/LCL155 possesses a binding activity lower than either BoNT/E or BuNT/BL5262. There was no difference in the catalytic activity of the three neurotoxins, which had been determined with a recombinant of the intracellular target protein SNAP-25. These data suggest that the BuNT/LCL155 shares the receptor-recognition site structurally different from BoNT/E and BuNT/BL5262, perhaps causing a decreased specific toxicity.
authors.affiliation: Department of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Tsukamoto
authors.forename: Masafumi
authors.initials: M
authors.lastname: Mukamoto
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kohda
authors.forename: Hideshi
authors.initials: H
authors.lastname: Ihara
authors.forename: Xingmin
authors.initials: X
authors.lastname: Wang
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Maegawa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Nakamura
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Karasawa
authors.forename: Shunji
authors.initials: S
authors.lastname: Kozaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001906:Botulism / Q000382:microbiology*; D003013:Clostridium / Q000472:pathogenicity*; D004797:Enzyme-Linked Immunosorbent Assay; D005516:Food Microbiology; D005732:Gangliosides / Q000378:metabolism; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry / Q000276:immunology / Q000633:toxicity*; D051381:Rats
chemical_list: D005732:Gangliosides; D009498:Neurotoxins