Construction of yellow fever-influenza A chimeric virus particles.
In order to obtain a better understanding of the functional mechanisms involved in the fusogenesis of enveloped viruses, the influenza A (X31) and the yellow fever (17DD) virus particles were used to construct a chimeric structure based on their distinct pH requirements for fusion, and the distinct malleability of their nucleocapsids. The malleable nucleocapsid of the influenza A virus particle is characterized by a pleomorphic configuration when observed by electron microscopy. A heat inactivated preparation of X31 virus was used as a lectin to interact with the sialic acid domains present in the 17DD virus envelope. The E spikes of 17DD virus were induced to promote fusion of both envelopes, creating a double genome enveloped structure, the chimeric yellow fever-influenza A virus particle. These chimeric viral particles, originally denominated 'partículas virais quiméricas' (PVQ), were characterized by their infectious capacity for different biological systems. Cell inoculation with PVQ resulted in viral products that showed similar characteristics to those obtained after 17DD virus infections. Our findings open new opportunities towards the understanding of both virus particles and aspects of cellular physiologic quality control. The yellow fever-influenza A chimeric particles, by means of their hybrid composition, should be a valuable tool in the study of cell biology and the function of viral components.
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Laboratório de Estruturas de Superfície de Vírus Envelopados e Interferons, Departamento de Virologia, Instituto de Microbiologia Professor Paulo de Góes, UFRJ, Caixa Postal, 68040 Rio de Janeiro, RJ, Brazil.
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D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D006863:Hydrogen-Ion Concentration; D009980:Influenza A virus / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D019251:Nucleocapsid; D014709:Vero Cells; D015005:Yellow fever virus / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure