The Göttingen minipig as a model for postprandial hyperlipidaemia in man: experimental observations.
Postprandial hyperlipidaemia is believed to be atherogenic. This study aimed to establish a minipig model to investigate determinants of postprandial lipid metabolism. In a randomized cross-over design seven minipigs were subjected to six different feeding regimens: intragastric fat loads of 1, 2, and 4 g fat (Intralipid, 20%) kg(-1) in two fractions 1.5 h apart (1/3 first, 2/3 second), 2 g fat (Intralipid kg(-1) in one fraction, and 2 g olive oil kg(-1) in two fractions, all after pre-feeding with standard diet, and finally 2 g fat (Intralipid kg(-1) in two fractions without pre-feeding. Blood was sampled before and hourly for 7 h after gavaging, and plasma triglycerides were measured. Triglycerides increased significantly in all the feeding regimens (P < 0.001), except when olive oil was used as the fat source. A borderline significant dose-response effect of the Intralipid dose on the triglyceride response was observed. We found no significant differences in triglyceride response whether 2 g fat (Intralipid kg(-1) was given in one or two fractions, with or without pre-feeding. We conclude that postprandial hyperlipidaemia in minipigs can be induced by gavaging an emulgated lipid solution (1-4 g fat/kg, Intralipid, while olive oil is not applicable. There is no need to administer the fat fractionated or to withhold food prior to administration.
Olsen
A K
AK
Department for Thrombosis Research, University of Southern Denmark, Ribe County Hospital, Esbjerg, Denmark. aako@ribeamt.dk
Bladbjerg
E M
EM
Marckmann
P
P
Larsen
L F
LF
Hansen
A K
AK
D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage / Q000009:adverse effects; D006949:Hyperlipidemias / Q000097:blood* / Q000209:etiology; D000069463:Olive Oil; D010938:Plant Oils / Q000008:administration & dosage / Q000009:adverse effects; D019518:Postprandial Period; D013552:Swine; D013556:Swine, Miniature / Q000502:physiology*; D013997:Time Factors; D014280:Triglycerides / Q000097:blood
D004041:Dietary Fats; D005217:Fat Emulsions, Intravenous; D000069463:Olive Oil; D010938:Plant Oils; D014280:Triglycerides