Simultaneous determination of dopa and carbidopa enantiomers by capillary zone electrophoresis.
Dopa and carbidopa, components of the dual therapy for Parkinson's disease treatment, are both provided as single enantiomers, since their D-forms are inactive. To ensure the efficiency and safety of the therapy, these D-enantiomers, therefore, should be considered as impurities. In this paper, the enantioseparation power of different types of cyclodextrins, both neutral and charged ones, on dopa and carbidopa enantiomers was tested. Three methods of simultaneous separation of dopa and carbidopa enantiomers were developed, using highly sulfated beta-cyclodextrin and sulfated beta-cyclodextrin as chiral selector, in normal and reversed polarity mode. Two methods among these three were found sensitive enough for the quantitation of 0.1% D-enantiomers in L-forms (impurity level). After the optimization study, the best method was selected, using 16 mM sulfated beta-cyclodextrin in 15 mM sodium phosphate buffer pH 2.45, an uncoated fused-silica capillary (50 num inner diameter, 30 cm total length), and an applied voltage of -12 kV. This method is robust and efficient, with very high resolution for all peaks within a short analysis time of 10 min. Quantitatively, the method offers a limit of detection (LOD) of 0.2 nug/mL and a limit of quantitation (LOQ) of 0.5 nug/mL for both D-dopa and D-carbidopa, which is equivalent to 0.02% and 0.05% against the respective L-enantiomers. A linear relationship was found between the concentration of the analyte and the corresponding peak area in a range of 0.5-2.0 nug/mL.
Ha
Pham Thi Thanh
PT
Laboratory for Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmaceutical Sciences, K. U. Leuven, Leuven, Belgium.
Van Schepdael
Ann
A
Hauta-Aho
Tuula
T
Roets
Eugene
E
Hoogmartens
Jos
J
D002230:Carbidopa / Q000032:analysis* / Q000737:chemistry*; D003505:Cyclodextrins / Q000032:analysis / Q000737:chemistry; D004295:Dihydroxyphenylalanine / Q000032:analysis* / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D003505:Cyclodextrins; D004295:Dihydroxyphenylalanine; D002230:Carbidopa