[High-level mIL-12 expression and replication of replicating adenovirus carrying mIL12 gene in naso-pharyngeal carcinoma cells].
OBJECTIVE
To enhance the therapeutic effects on nasopharyngeal carcinoma by combining treatment with selectively replicating adenovirus and IL12.


METHODS
Replicating adenovirus with mouse IL12 gene insert (CNHK200-mIL12) was constructed to transfect nasopharyngeal carcinoma cell lines CNE3 and 915. Adenovirus hexon was detected by immunohistochemical staining and flow cytometry (FCM), and mIL12 expression examined by enzyme-linked immunosorbent assay (ELISA). The replication rates of CNHK200-mIL12 and dl1520 was determined by 50% tissue culture infectious dose (TCID50).


RESULTS
Twenty-four hours after transfection with CNHK200-mIL12, most of cells were positive for adenovirus hexon and FCM demonstrated increased positivity rates of 39% and 4% among CNE3 and 915 cells respectively. It was observed that CNHK200-mIL12 replication increased by 1 000 folds with mIL12 expression level reaching as high as 84.5+/-4.6 ng in CNE3 cells and 75.6+/-3.4 ng in 915 cells as determined 72 h after transfection with 1x10(5) PFU CNHK200-mIL12 into 1x10(4) cells.


CONCLUSIONS
CNHK200-mIL12 can replicate in vitro in nasopharyngeal carcinoma cells (dl1520 cells, for instance) with high mIL12 expression, which suggests that CNHK200-mIL12 may potentially be used to treat nasopharyngeal carcinoma.
Nie
Biao
B
Central Laboratory, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Qian
Qi-Jun
QJ
Che
Xiao-Yan
XY
Xue
Hui-Bin
HB
Sham
Jonathan
J
D000256:Adenoviridae / Q000235:genetics / Q000502:physiology; D000818:Animals; D015870:Gene Expression; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000473:pathology* / Q000628:therapy; D019076:Transgenes / Q000502:physiology*; D014779:Virus Replication
D018664:Interleukin-12