Voltammetric determination of nitrosocimetidine in simulated gastric juice.
OBJECTIVE
To develop a voltammetric method for the determination of nitrosocimetidine in simulated gastric juice (SGJ).


METHODS
Measurement of the polarographic wave of the nitroso derivative at the dropping mercury electrode in 0.1 m HCl.


RESULTS
A well-defined diffusion-controlled cathodic wave was obtained at -0.34 V vs. Ag/AgCl electrode. The current-concentration relationship was found to be rectilinear over the range 10-80 microg/mL and 2-50 microg/mL with percentage recovery of 101.24 +/- 0.79 and 100.79 +/- 0.56 for the direct current (DCt) and differential pulse polarography (DPP) modes, respectively, with a minimum detectability (S/N=2) of 0.16 microg/ml (5.6 x 10-7 m) using DPP mode.


CONCLUSIONS
The proposed method was successfully applied to study the nitrosation of cimetidine in SGJ.
Walash
M I
MI
Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt. manaleid@mum.mans.eun.eg
Belal
F
F
Ibrahim
F
F
Hefnawy
M
M
Eid
M
M
D002927:Cimetidine / Q000031:analogs & derivatives* / Q000032:analysis*; D005750:Gastric Juice / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015538:Nitrosation / Q000187:drug effects; D011048:Polarography / Q000379:methods*; D013997:Time Factors
C019503:nitrosocimetidine; D002927:Cimetidine