Expression of 5-HT(2A) receptor mRNA in some nuclei of brain stem enhanced in monoarthritic rats.
The present study was designed to investigate the expression of 5-hydroxytryptamine (5-HT) (2A) receptor mRNA in the nucleus of raphe magnus (NRM), ventrolateral periaqueductal gray (vlPAG) and dorsal raphe nucleus (DRN) in a monoarthritic rat model using an in situ hybridization technique. 5-HT(2A) receptor mRNA was expressed with low to moderate levels in NRM, vlPAG and DRN. After complete Freund's adjuvant (CFA)-induced monoarthritis, a significant increase in 5-HT(2A) receptor mRNA expression was observed in bilateral NRM, vlPAG and DRN, and the change lasted, at least, for 2 weeks. This result indicates that the synthesis of 5-HT(2A) receptor is enhanced in bilateral NRM, vlPAG and DRN of monoarthritic rats, suggesting that 5-HT(2A) receptor is involved in the central regulation of pain transmission following CFA-induced chronic inflammation.
Xie
Hong
H
Department of Neurobiology, State Key Laboratory of Medical Neurobiology, Medical Center of Fudan University (Former Shanghai Medical University), P.O. Box 291, 138 Yi-Xue-Yuan Road, Shanghai 200032, People's Republic of China.
Ma
Fei
F
Zhang
Yu-Qiu
YQ
Gao
Xiu
X
Wu
Gen-Cheng
GC
D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D001933:Brain Stem / Q000378:metabolism*; D015870:Gene Expression; D017403:In Situ Hybridization; D008297:Male; D010147:Pain Measurement; D010487:Periaqueductal Gray / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D011903:Raphe Nuclei / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044402:Receptor, Serotonin, 5-HT2A; D011985:Receptors, Serotonin / Q000096:biosynthesis*
D012333:RNA, Messenger; D044402:Receptor, Serotonin, 5-HT2A; D011985:Receptors, Serotonin