title: Molecular characterization of ribonucleotide reductase from Cryptosporidium parvum.
abstract: The Apicomplexan enteric parasite, Cryptosporidium, infects a broad range of mammals, birds, fish and reptiles. Cryptosporidium parvum, the principal causative agent of human cryptosporidiosis, has emerged as a major contributor to waterborne outbreaks of this disease. The absence of effective drugs against C. parvum has necessitated the search for new drug targets. One attractive class of target enzymes is ribonucleotide reductase, an essential enzyme required for the de novo synthesis of deoxyribonucleotides. We report the cloning and sequencing of the small and large subunits of ribonucleotide reductase from a C. parvum genotype 2 isolate, GCH1. Southern blot analysis showed that these subunits are encoded by single copy genes. Both subunits have been expressed as recombinant proteins in Escherichia coli.
authors.affiliation: Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, MA 01536, USA. donna.akiyoshi@tufts.edu
authors.forename: Donna E
authors.initials: DE
authors.lastname: Akiyoshi
authors.forename: Ramaswamy
authors.initials: R
authors.lastname: Balakrishnan
authors.forename: Christian
authors.initials: C
authors.lastname: Huettinger
authors.forename: Giovanni
authors.initials: G
authors.lastname: Widmer
authors.forename: Saul
authors.initials: S
authors.lastname: Tzipori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016785:Cryptosporidium parvum / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010961:Plasmodium / Q000235:genetics; D012264:Ribonucleotide Reductases / Q000235:genetics*; D016415:Sequence Alignment; D014345:Trypanosoma / Q000235:genetics
chemical_list: D012264:Ribonucleotide Reductases