Insights into the biology of HIV-1 viral protein R.
HIV-1 viral protein R (Vpr) is a small, highly conserved accessory protein encoded by the HIV genome that serves many functions in the viral life cycle. Vpr induces G2 cell cycle arrest, which is thought to indirectly enhance viral replication by increasing transcription from the LTR. Vpr has also been implicated in facilitating infection of nondividing cells, most notably macrophages. Because Vpr is a nucleo-cytoplasmic shuttling protein, its role in enhancing viral replication in macrophages may be mediated through enhanced entry of the HIV preintegration complex through the limiting nuclear pore. Free Vpr is detectable in the serum of patients, and in vitro studies implicate extracellular forms of Vpr as an effector of cellular responses mediated through its ability to transduce through intact cytoplasmic membranes. We review the biologic properties of Vpr, focusing on its mechanism of action, role in HIV replication, and significance for host pathogenesis.
Sherman
Michael P
MP
Gladstone Institute of Virology and Immunology and Department of Medicine, University of California, San Francisco, California 94141-9100, USA.
De Noronha
Carlos M C
CM
Williams
Samuel A
SA
Greene
Warner C
WC
D021581:Active Transport, Cell Nucleus; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D002467:Cell Nucleus / Q000821:virology; D016213:Cyclins / Q000378:metabolism; D016336:Gene Products, vpr / Q000502:physiology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D054325:vpr Gene Products, Human Immunodeficiency Virus
D016213:Cyclins; D016336:Gene Products, vpr; D054325:vpr Gene Products, Human Immunodeficiency Virus; D016203:CDC2 Protein Kinase