title: Genetic variability in the coat protein genes of two orchid viruses: Cymbidium mosaic virus and Odontoglossum ringspot virus.
abstract: The variability in coat protein gene sequences of Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) that naturally infect orchids worldwide was investigated. Samples were collected from Korea, Singapore and Taiwan. The sequence data were compared with available published coat protein gene sequences of CymMV and ORSV, including those from Japan and Thailand. Among CymMV isolates, the homology was 89.1%-99.7% and 93.2%-100% at the nucleotide and amino acid levels, respectively. Among the ORSV isolates, the homology was 95.5%-100% and 93%-100% at the nucleotide and amino acid levels, respectively. No particular region of variability could be defined in either of the viruses. In deduced amino acid sequence, the N-terminal was more conserved than the C-terminal in both CymMV and ORSV. By comparing all sequences determined in this study and those that are published in the GenBank databases, we did not find clustering based on geographical distribution or sequence identity. Such high sequence conservation suggests that both CymMV and ORSV coat protein genes are suitable candidates to provide resistance to orchids cultivated in different geographical locations.
authors.affiliation: Department of Biological Sciences, The National University of Singapore, Kent Ridge, Singapore.
authors.forename: P A
authors.initials: PA
authors.lastname: Ajjikuttira
authors.forename: C L
authors.initials: CL
authors.lastname: Lim-Ho
authors.forename: M H
authors.initials: MH
authors.lastname: Woon
authors.forename: K H
authors.initials: KH
authors.lastname: Ryu
authors.forename: C A
authors.initials: CA
authors.lastname: Chang
authors.forename: C S
authors.initials: CS
authors.lastname: Loh
authors.forename: S M
authors.initials: SM
authors.lastname: Wong
mesh_terms: D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D017863:Potexvirus / Q000235:genetics*; D016415:Sequence Alignment; D017898:Tobamovirus / Q000235:genetics*
chemical_list: D036022:Capsid Proteins