Gene encoding a trehalose phosphorylase from Thermoanaerobacter brockii ATCC 35047.
A gene encoding a trehalose phosphorylase was cloned from Thermoanaerobacter brockii ATCC 35047. The gene encodes a polypeptide of 774 amino acid residues. The deduced amino acid sequence was homologous to bacterial maltose phosphorylases and a trehalose 6-phosphate phosphorylase catalyzing anomer-inverting reactions. On the other hand, no homology was found between the T. brockii enzyme and an anomer-retaining trehalose phosphorylase from Grifola frondosa.
Maruta
Kazuhiko
K
Amase Institute, Hayashibara Biochemical Laboratories, Inc, Okayama, Japan. amaseken@hayashibara.co.jp
Mukai
Kazuhisa
K
Yamashita
Hiroshi
H
Kubota
Michio
M
Chaen
Hiroto
H
Fukuda
Shigeharu
S
Kurimoto
Masashi
M
D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D005964:Glucosyltransferases; C023181:alpha,alpha-trehalose phosphorylase