Clinical strain of Pseudomonas aeruginosa carrying a bla(TEM-21) gene located on a chromosomal interrupted TnA type transposon.
A clinical isolate of Pseudomonas aeruginosa was found to produce a clavulanic acid-inhibited extended-spectrum beta-lactamase with a pI of 6.4. PCR, cloning, and sequencing experiments showed that the corresponding bla(TEM-21) gene was part of a chromosomally located Tn801 transposon disrupted by an IS6100 element and adjacent to an aac(3)-II gene.
Dubois
Véronique
V
Laboratoire de Microbiologie, Faculté de Pharmacie, Université de Bordeaux 2, Bordeaux, France. veronique.dubois@bacterio.u-bordeaux2.fr
Arpin
Corinne
C
Noury
Patrick
P
Quentin
Claudine
C
D000368:Aged; D000904:Antibiotics, Antitubercular / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D002876:Chromosomes, Bacterial / Q000187:drug effects / Q000235:genetics*; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012293:Rifampin / Q000494:pharmacology; D001618:beta-Lactamases / Q000235:genetics*
D000904:Antibiotics, Antitubercular; D001386:Azides; D004269:DNA, Bacterial; D001618:beta-Lactamases; C472386:beta-lactamase TEM-21, Pseudomonas aeruginosa; D012293:Rifampin