Vasoactive intestinal peptide regulation of nerve growth factor in the embryonic mouse.
Vasoactive intestinal peptide (VIP), a regulator of embryonic growth, increased the concentration of nerve growth factor (NGF)-like immunoreactivity in the conditioned medium of cultured explanted embryonic day (E) 9.5 neural tube preparations compared to control preparations. VIP treatment also induced an increase of NGF-like immunoreactivity (NGF-IR) within the neural tube preparation tissue. A 60 kDa isoform was the primary form of NGF detected. VIP is shown to be a regulator of NGF in the E9.5 embryonic mouse and stimulates the release of a high molecular weight isoform of NGF.
Hill
Joanna M
JM
Section on Developmental and Molecular Pharmacology, Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA. jh139h@nih.gov
Mehnert
Janice
J
McCune
Susan K
SK
Brenneman
Douglas E
DE
D000818:Animals; D017077:Culture Media, Conditioned; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D051379:Mice; D020932:Nerve Growth Factor / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D020033:Protein Isoforms / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D017077:Culture Media, Conditioned; D020033:Protein Isoforms; D014660:Vasoactive Intestinal Peptide; D020932:Nerve Growth Factor; D007770:L-Lactate Dehydrogenase