Maturation of dendritic cells induced by Candida beta-D-glucan.
We investigated whether Candida beta-D-glucan (CSBG) alters the maturation of dendritic cells (DCs). DC phenotypes were analyzed using FACScan. The expression of surface molecules, including major histocompatibility complex (MHC) classes I and II, as well as CD80 and CD86, increased on DCs that were stimulated with lipopolysaccaride (DCs/LPS), in comparison with unstimulated bone marrow-derived DCs (BM-DCs). Furthermore, the level of surface molecule expression on DCs stimulated with CSBG (DCs/CSBG) was between that of DCs and DCs/LPS. Phagocytosis was assessed by the uptake of FITC-dextran. There were no differences in the uptake of dextran among DCs/LPS and DCs/CSBG. The ability of BM-DCs to uptake dextran was higher than that of DCs/LPS and DCs/CSBG. We analyzed the concentration of IL-12 secreted by DCs using ELISA. BM-DCs secreted a low concentration of IL-12, while DCs/LPS and DCs/CSBG secreted higher levels of IL-12 than BM-DCs. There were no remarkable differences in the concentrations of IL-12 produced by DCs/LPS and DCs/CSBG. This data suggests that CSBG may augment DC maturation.
Kikuchi
Tetsuro
T
Department of Oncology, The Institute of DNA Medicine, Jikei University, Tokyo, Japan. tkikuchi@jikei.ac.jp
Ohno
Naohito
N
Ohno
Tsuneya
T
D000818:Animals; D002175:Candida / Q000737:chemistry*; D003713:Dendritic Cells / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005936:Glucans / Q000494:pharmacology*; D018664:Interleukin-12 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010873:Pinocytosis; D047071:beta-Glucans
D005936:Glucans; D047071:beta-Glucans; D018664:Interleukin-12; C064197:beta-1,6-glucan; C033363:beta-1,3-glucan