[Effects of lipofectin-mediated alpha1,4Gal T antisense oligonucleotide on human glioma cell line SWO-38].
OBJECTIVE
To study the effects of alpha1,4Gal T antisense oligonucleotide mediated by lipofectin on human glioma cell line SWO-38.


METHODS
SWO38 glioma cells were exposed to 10 micromol/L alpha1,4Gal T antisense oligonucleotide for 72 h by means of lipofectin transfection, and the growth inhibition of the cells was detected by colony-forming unit assay. Analysis of DNA fragmentation was performed with flow cytometry (FCM) and agarose gel eletrophoresis with Fas protein expression determined by FCM.


RESULTS
alpha1,4Gal T antisense oligonucleotide significantly inhibited the growth of glioma cells (P<0.01) and induced apoptosis in SWO-38 cells (P<0.05). Marked up-regulation of Fas protein expression was observed in response to the treatment (P<0.01).


CONCLUSIONS
alpha1,4Gal T antisense oligonucleotide can significantly inhibit SWO-38 cell proliferation and induce cellular apoptosis, the mechanism of which may involve up-regulated Fas expression.
Zheng
Gang
G
Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Zhang
Ji-Ren
JR
Liu
Fa-Quan
FQ
Rao
Zhi-Guo
ZG
Wang
Xiong-Wen
XW
Zhang
Xin-Yu
XY
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D005700:Galactosyltransferases / Q000037:antagonists & inhibitors* / Q000235:genetics; D005910:Glioma / Q000473:pathology*; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay; D015854:Up-Regulation; D019014:fas Receptor / Q000378:metabolism
D000970:Antineoplastic Agents; D016376:Oligonucleotides, Antisense; D010714:Phosphatidylethanolamines; D019014:fas Receptor; C020888:1,2-dielaidoylphosphatidylethanolamine; D005700:Galactosyltransferases; C045097:UDP-galactose-lactosylceramide alpha 1-4-galactosyltransferase