[Investigation of practical application of fluorescence in situ hybridization (FISH) analysis using microwave irradiation in formalin-fixed, paraffin-embedded tissue sections].
We investigated fluorescence in situ hybridization (FISH) analysis using microwave irradiation in formalin-fixed, paraffin-embedded tissue sections of breast fibroadenoma. Higher percentage of cells with 2 signal copies of chromosome 3 centromere could be obtained in the condition of 5 microns thick sections, when we counted cells of more than 4 microns of nuclei in thickness. This method showed about the same results as FISH using cells separated from the same tissues. Percentage of cells with 2 signal copies of chromosome 17 centromere in 14 cases was 80.6 +/- 4.0% (Mean +/- S.D.). This method is expected in the application of the prognosis estimation of the breast cancer.
Kurosou
Kazuko
K
Department of Pathology, Dokkyo University School of Medicine, Koshigaya Hospital, Koshigaya 343-8555.
Murakami
Shigeru
S
Jinnai
Mutsuo
M
Koba
Hiroyuki
H
Imai
Yuuko
Y
Yamada
Takashi
T
Ueda
Yoshihiko
Y
Mori
Yoshiomi
Y
D001943:Breast Neoplasms / Q000175:diagnosis*; D018226:Fibroadenoma / Q000175:diagnosis; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008872:Microwaves; D016612:Paraffin Embedding; D011379:Prognosis; D016707:Tissue Fixation