title: Orientation of ribosome recycling factor in the ribosome from directed hydroxyl radical probing.
abstract: Ribosome recycling factor (RRF) disassembles posttermination complexes in conjunction with elongation factor EF-G, liberating ribosomes for further rounds of translation. The striking resemblance of its L-shaped structure to that of tRNA has suggested that the mode of action of RRF may be based on mimicry of tRNA. Directed hydroxyl radical probing of 16S and 23S rRNA from Fe(II) tethered to ten positions on the surface of E. coli RRF constrains it to a well-defined location in the subunit interface cavity. Surprisingly, the orientation of RRF in the ribosome differs markedly from any of those previously observed for tRNA, suggesting that structural mimicry does not necessarily reflect functional mimicry.
authors.affiliation: Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Lancaster
authors.forename: Michael C
authors.initials: MC
authors.lastname: Kiel
authors.forename: Akira
authors.initials: A
authors.lastname: Kaji
authors.forename: Harry F
authors.initials: HF
authors.lastname: Noller
mesh_terms: D004926:Escherichia coli / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D012338:RNA, Ribosomal, 23S / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012269:Ribosomal Proteins; D012270:Ribosomes / Q000737:chemistry*
chemical_list: D011506:Proteins; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D012269:Ribosomal Proteins; C087267:ribosome releasing factor; D017665:Hydroxyl Radical; D012343:RNA, Transfer