Homologous recombination: ends as the means.
Broken chromosomal ends in somatic cells of higher plants frequently heal by the ligation of DNA ends to unrelated sequences or to sequences with micro-homologies. This pathway of DNA-strand-break repair is the bane of gene-targeting attempts in plants. However, there is a second somatic pathway of chromosome repair, which is driven by DNA-sequence homology. Observations from yeast, fly and plants of homologous-recombination mechanisms point towards new strategies of gene targeting in plants.
Ray
Animesh
A
Keck Graduate Institute, 535 Watson Drive, Claremont, CA 91711, USA. animesh_ray@kgi.edu
Langer
Marybeth
M
D029681:Arabidopsis Proteins / Q000235:genetics; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D032461:Chromosomes, Plant / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics*; D008540:Meiosis / Q000235:genetics; D008957:Models, Genetic; D009154:Mutation; D011995:Recombination, Genetic / Q000235:genetics*; D018741:Replication Origin / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics
D029681:Arabidopsis Proteins; D002843:Chromatin; C427727:rad50 protein, Arabidopsis