title: Properties of a chimeric simian-human immunodeficiency virus expressing an hybrid HIV-1 Nef/SIVmac Nef protein.
abstract: The nef genes of human and simian immunodeficiency viruses code for a membrane associated protein critical for AIDS development. SIVmac Nef presents C-terminal a 27 amino acid extension absent of HIV-1 Nef. To estimate the influence of this C-terminal domain on virus properties, we constructed viruses derived from SIVmac239 by replacing SIV nef with HIV-1 Lai nef gene (SHIV NefLai4) or with a sequence encoding a Nef fusion protein: HIV-1 Lai Nef/SIV Nef-Cterm (SHIV-Cterm). The recombinant viruses replicated efficiently in vitro in CEMx174 cells and in activated macaque PBMCs. The addition of SIV Nef C-terminal domain to HIV-1 Nef in SHIVNefLai4 did not change the in vitro properties of the chimeric virus, both viruses being more infectious than a nef deleted virus. Although the half-life of Nef fusion protein was augmented, SHIV-Cterm remained slightly less infectious than SIVmac239.
authors.affiliation: INSERM U544, Institut de Virologie, Université Louis Pasteur, Strasbourg, France.
authors.forename: C
authors.initials: C
authors.lastname: Bertsch
authors.forename: D
authors.initials: D
authors.lastname: Cluet
authors.forename: C
authors.initials: C
authors.lastname: Beyer
authors.forename: L
authors.initials: L
authors.lastname: Gloeckler
authors.forename: A
authors.initials: A
authors.lastname: Cecile
authors.forename: J P
authors.initials: JP
authors.lastname: Gut
authors.forename: A M
authors.initials: AM
authors.lastname: Aubertin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015754:Genes, nef; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity; D008251:Macaca; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000472:pathogenicity; D054334:Viral Regulatory and Accessory Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D014779:Virus Replication
chemical_list: C429284:NEF protein, SIV; D011993:Recombinant Fusion Proteins; D054334:Viral Regulatory and Accessory Proteins