Effect of beer on the plasma concentrations of uridine and purine bases.
We conducted the present study to determine whether beer, both with and without ethanol content, increases the plasma concentration and urinary excretion of purine bases and uridine. Because 10 mL of regular beer (with ethanol) was found to contain 0.34 g of freeze-dried beer (without ethanol) and 0.5 mg of uridine, 5 healthy males were given regular beer (10 mL/kg of body weight) and freeze-dried beer (0.34 g/kg of body weight) or uridine (0.5 mg/kg of body weight). The plasma concentrations of hypoxanthine, xanthine, and uridine increased by 3.5-fold (P <.05), 4.7-fold (P <.05), and 1.8-fold (P <.05), respectively, 30 minutes after regular beer ingestion, and the urinary excretion of hypoxanthine, xanthine, and uridine increased by 4.0-fold (P <.05), 4.5-fold (P <.01), and 1.7-fold (P <.05), respectively, when measured 1 hour after ingestion. The plasma concentrations of uric acid and total purine bases increased by 6.5% (P <.05) and 7.6% (P <.05), respectively, 30 minutes after regular beer ingestion, whereas the urinary excretion of uric acid did not increase, while that of total purine bases increased by 1.3-fold (P <.05) when measured 1 hour after ingestion. As for freeze-dried beer, the plasma concentrations of uric acid total purine bases increased by 4.4% (P <.05) and 4.6% (P <.05), respectively, and that of uridine by 1.5-fold (P <.01) 30 minutes after ingestion, while the urinary excretion of uridine increased by 1.4-fold (P <.01) 1 hour after ingestion. However, the plasma concentrations and urinary excretion of hypoxanthine and xanthine and the urinary excretion of uric acid and total purine bases did not change significantly. As for uridine ingestion, the plasma concentration of uridine increased by 1.37-fold (P <.01) 30 minutes after ingestion, and the urinary excretion of uridine increased by 1.3-fold (P <.01) 1 hour after ingestion. However, the plasma concentrations and urinary excretion of hypoxanthine, xanthine, uric acid, and total purine bases did not change significantly. These results suggest that the purines in beer played a major role in the increase in the plasma concentration of uric acid, while both uridine and ethanol in beer had a significant effect on the increase in plasma concentration of uridine.
Yamamoto
Tetsuya
T
Division of Endocrinology and Metabolism, Department of Internal Medicine, Hyogo College of Medicine, Hyogo, Japan.
Moriwaki
Yuji
Y
Takahashi
Sumio
S
Tsutsumi
Zenta
Z
Ka
Tsuneyoshi
T
Fukuchi
Minoru
M
Hada
Toshikazu
T
D000328:Adult; D001515:Beer / Q000009:adverse effects* / Q000032:analysis; D002492:Central Nervous System Depressants / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000494:pharmacology; D005612:Freeze Drying; D006801:Humans; D019271:Hypoxanthine / Q000097:blood / Q000652:urine; D019344:Lactic Acid / Q000097:blood; D008297:Male; D008875:Middle Aged; D011687:Purines / Q000032:analysis / Q000097:blood*; D011743:Pyrimidines / Q000032:analysis; D019289:Pyruvic Acid / Q000097:blood; D013056:Spectrophotometry, Ultraviolet; D014527:Uric Acid / Q000097:blood / Q000652:urine; D014529:Uridine / Q000097:blood* / Q000652:urine; D014970:Xanthines / Q000097:blood / Q000652:urine
D002492:Central Nervous System Depressants; D011687:Purines; D011743:Pyrimidines; D014970:Xanthines; D014527:Uric Acid; D019271:Hypoxanthine; D019344:Lactic Acid; D000431:Ethanol; D019289:Pyruvic Acid; D014529:Uridine