title: Expression of exogenous rat collagenase in vitro and in a rat model of liver fibrosis.
abstract: OBJECTIVE
  The present study was conducted to test the hypothesis that the introduction of the collagenase gene into tissue culture cells and into a rat model of liver fibrosis would result in the expression of enzymatically active product.
  
  
  METHODS
  FLAG-tagged full-length rat collagenase cDNA was PCR amplified and cloned into a mammalian expression vector. NIH3T3 cells were then transiently transfected with this construct. Expression of exogenous collagenase mRNA was assessed by RT-PCR, and the exogenous collagenase detected by Western blotting using anti-FLAG monoclonal antibody. Enzymatic activity was detected by gelatin zymography. To determine the effects of exogenous collagenase production in vivo, the construct was bound to glycosyl-poly-L-lysine and then transduced into rats that had developed liver fibrosis as a result of CCl(4) plus ethanol treatment. The hepatic expression of the construct and its effect on the formation of liver fibrosis were demonstrated using RT-PCR and immunohistochemistry.
  
  
  RESULTS
  It was found that exogenously expressed rat collagenase mRNA could be detected in NIH3T3 cells following transfection. Enzymatically active collagenase could also be detected in the culture medium. The recombinant plasmid was also expressed in rat liver after in vivo gene transfer. Expression of exogenous rat collagenase correlated with decreased deposition of collagen types I and III in the livers of rats with experimentally induced liver fibrosis.
  
  
  CONCLUSIONS
  The expression of active exogenous rat collagenase could be achieved in vitro and in vivo. It was suggested that in vivo expression of active exogenous collagenase may have therapeutic effects on the formation of liver fibrosis.
authors.affiliation: Division of Gastroenterology, Zhongshan Hospital, Fu Dan University, Shanghai 200032, China. jiyao_wang@hotmail.com
authors.forename: Ji-Yao
authors.initials: JY
authors.lastname: Wang
authors.forename: Jin-Sheng
authors.initials: JS
authors.lastname: Guo
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Yang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D017364:Collagenases / Q000235:genetics*; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology; D008103:Liver Cirrhosis / Q000201:enzymology* / Q000503:physiopathology*; D008297:Male; D051379:Mice; D010957:Plasmids; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection
chemical_list: D017364:Collagenases