Functional mimicry between anti-Tendamistat antibodies and alpha-amylase.
A proteinaceous inhibitor of alpha-amylase, Tendamistat, was evaluated as an immunogen to induce antibodies that mimic the enzyme activity and to investigate a new strategy to produce catalytic antibodies. Anti-Tendamistat polyclonal antibodies (pAbs) were shown to cross-react with acarbose, a strong carbohydrate inhibitor of alpha-amylases, and the alpha-amylase carbohydrate substrates, maltotetraose and maltoheptaose. Catalytic features of pAbs were characterized after denaturing natural serum alpha-amylase by treatment at pH 10 and in the presence of EDTA. A significant residual alpha-amylase activity was detected and associated with the IgG fraction, demonstrating that some antibodies behave as a functional mimic of natural amylase. Such antibodies, which behave as an "internal image" of the alpha-amylase, were used as immunogens to elicit anti-idiotype antibodies. It was demonstrated that the anti-idiotype antiserum contains a significant amount of antibodies that bind to porcine pancreatic amylase, which shows that they contain structural information from the original hapten, Tendamistat.
Gonçalves
Olivier
O
FRE-CNRS No. 2230 Biocatalyse, Faculté des Sciences et des Techniques 2, rue de la Houssinière, BP 92208, 44322 Nantes Cedex 03, France.
Dintinger
Thierry
T
Blanchard
Dominique
D
Tellier
Charles
C
D020909:Acarbose / Q000276:immunology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism; D017151:Antibodies, Catalytic / Q000096:biosynthesis / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D002384:Catalysis; D003429:Cross Reactions; D004791:Enzyme Inhibitors / Q000276:immunology*; D005936:Glucans / Q000378:metabolism; D008320:Maltose / Q000031:analogs & derivatives / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D009844:Oligosaccharides / Q000378:metabolism; D010455:Peptides / Q000276:immunology*; D011817:Rabbits; D000516:alpha-Amylases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000888:Antibodies, Anti-Idiotypic; D017151:Antibodies, Catalytic; D004791:Enzyme Inhibitors; D005936:Glucans; D009844:Oligosaccharides; D010455:Peptides; C031146:maltoheptaose; D008320:Maltose; C009819:maltotetraose; D000516:alpha-Amylases; D020909:Acarbose; C030526:tendamistate