title: A new method for determination of buformin in plasma and urine by ion-paired reversed-phase HPLC with ultraviolet detection.
abstract: Buformin is a widely used as an antidiabetic agent but its renal excretion is still controversial. A new HPLC method with ultraviolet (UV) detection for the determination of buformin in plasma and urine has been developed. After protein precipitation or dilution, buformin and internal standard phenformin were resolved on an octadecyl silica column and detected by UV detection at 233 nm. Intra- and inter-day coefficients of variation were <9%. The limit of quantification was around 0.05 micro g/ml for plasma and 2.5 micro g/ml for urine.
authors.affiliation: Department of Pharmacy, The University of Tokyo Hospital, Faculty of Medicine, The University of Tokyo, 3-1 Hongo 7-chome, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Takehito
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Makiko
authors.initials: M
authors.lastname: Kusama
authors.forename: Kumi
authors.initials: K
authors.lastname: Matsuno
authors.forename: Erika
authors.initials: E
authors.lastname: Sugiyama
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Yamada
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Iga
mesh_terms: D002026:Buformin / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D007004:Hypoglycemic Agents; D002026:Buformin