Active substances against trypomastigote forms of Trypanosoma cruzi and microorganisms are produced in sequence by Talaromyces flavus.
The conditions for the sequential production of antibiotic activity by Talaromyces flavus were determined. The highest level of activity against Trypanosoma cruzi was obtained from the aqueous extract of the Czapeck's fermentative culture after 48 hours, with lysis of 97.58% of the trypomastigote forms of Trypanosoma cruzi (red blood cells remained normal). The antimicrobial activity was detected in the extracts of fermentative cultures from different media just after 144 hours of incubation. Maximum activities against Micrococcus luteus, Staphylococcus aureus and Candida albicans were present in chloroform, butanolic and water extracts, in this order, when Talaromyces flavus was cultivated at pH 5.0. The minimal inhibitory concentration (MIC) of extracts of Takeuchi's cultures were determined.
Freitas
Tatiana P S
TP
Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil.
Furtado
Niege A J C
NA
Bastos
Jairo K
JK
Said
Suraia
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D005949:Glucose Oxidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D016982:Micrococcus luteus / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D032901:Talaromyces / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D005949:Glucose Oxidase