Detection of ganglion cells in the colonic plexuses by immunostaining for neuron-specific marker NeuN: an aid for the diagnosis of Hirschsprung disease.
The majority of ganglion cells in the colonic plexuses can be easily and specifically identified by immunostaining for neuronal marker NeuN. The distance between the neighboring solitary ganglion cells or groups of ganglion cells varied from 0.18 to 4.0 mm, average 1.0 mm, in ganglionic segments of colons of patients with Hirschsprung disease, and from 0.3 to 6.3 mm, average 1.43 mm, in colons of pediatric patients with chronic constipation of various etiologies. No ganglion cells were detected in aganglionic colonic segments of patients with Hirschsprung disease by this method.
Yang
Shiwen
S
Department of Pathology, Scott and White Clinic and Memorial Hospital, Temple, Texas 76508, USA.
Donner
Ludvik R
LR
D015415:Biomarkers; D002675:Child, Preschool; D003106:Colon / Q000294:innervation* / Q000378:metabolism / Q000473:pathology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005724:Ganglia / Q000378:metabolism / Q000473:pathology*; D006627:Hirschsprung Disease / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007223:Infant; D007231:Infant, Newborn; D009419:Nerve Tissue Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D015415:Biomarkers; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors