Reactive oxygen species mediate leukocyte-endothelium interactions in prostaglandin F2alpha -induced luteolysis in rats.
We investigated the contribution of neutrophils to prostaglandin (PG)F(2 alpha)-induced luteolysis and the role of reactive oxygen species (ROS) as potential mediators of neutrophil accumulation in regressing corpora lutea of superovulated rats. On day 8 of pseudopregnancy, subcutaneous injection of PGF(2 alpha) (500 microg) significantly increased rhodamine 6G-labeled leukocyte adhesion in luteal venules, as observed by intravital microscopy. Neutrophil accumulation was confirmed by significantly increased myeloperoxidase (MPO) activity. Pretreatment with anti-leukocyte antibody (CD18-directed monoclonal antibody, WT-3) significantly inhibited the PGF(2 alpha)-induced increases in adherent leukocytes and MPO activity. Anti-leukocyte antibody also maintained serum progesterone concentrations. Pretreatment with oxygen free radical scavengers, superoxide dismutase (50,000 U/kg) and catalase (90,000 U/kg), also attenuated these PGF(2 alpha)-induced alterations. Corpora lutea preloaded with dichlorodihydrofluorescein diacetate succinimidyl ester, a fluorescent indicator for determining intracellular ROS generation, exhibited an increase in fluorescence after PGF(2 alpha) treatment. These findings suggest that leukocyte-endothelium interactions mediated by ROS generation are important in PGF(2 alpha)-induced luteolysis in rats.
Minegishi
Kazuhiro
K
Department of Obstetrics and Gynecology, Keio University School of Medicine, 160-8582 Tokyo, Japan.
Tanaka
Mamoru
M
Nishimura
Osamu
O
Tanigaki
Shinji
S
Miyakoshi
Kei
K
Ishimoto
Hitoshi
H
Yoshimura
Yasunori
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018821:CD18 Antigens / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D003338:Corpus Luteum / Q000098:blood supply / Q000166:cytology / Q000187:drug effects; D015237:Dinoprost / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D003341:Luteolysis / Q000187:drug effects / Q000502:physiology*; D008833:Microcirculation / Q000166:cytology / Q000502:physiology; D018715:Microscopy, Video; D020556:Neutrophil Infiltration / Q000187:drug effects / Q000502:physiology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014699:Venules / Q000166:cytology / Q000502:physiology
D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D015237:Dinoprost