Novel candidate tumor marker genes for lung adenocarcinoma.
Using the representational difference analysis (RDA) technique on pooled mRNA of five primary lung adenocarcinomas and their corresponding non-neoplastic lung tissues, we identified six genes that were putatively overexpressed in this type of lung cancer. Five corresponded to previously isolated genes, while one (Lc19) matched with the sequence of an unannotated EST. Real-time RT-PCR analyses of expression levels in a panel of 34 paired primary non-small cell lung cancer (NSCLC) and corresponding grossly normal appearing lung tissues confirmed the common overexpression of these genes in non-small cell lung cancer. Among these genes, overexpression of Lc19, hyaluronan binding protein 2 (HABP2) and crystalline-mu appeared more specific to adenocarcinoma, whereas ceruloplasmin, integrin alpha-11 and collagen type XI alpha 1 were overexpressed at high frequency among both adenocarcinoma and squamous cell carcinoma. These genes represent novel candidate tumor biomarker genes for NSCLC and its histological subtypes.
Wang
Kan-Kan
KK
University Health Network - Ontario Cancer Institute/Princess Margaret Hospital, Toronto, Ontario, Canada.
Liu
Ni
N
Radulovich
Nikolina
N
Wigle
Dennis A
DA
Johnston
Michael R
MR
Shepherd
Frances A
FA
Minden
Mark D
MD
Tsao
Ming-Sound
MS
D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D001483:Base Sequence; D014408:Biomarkers, Tumor; D017931:DNA Primers; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D017931:DNA Primers; D005819:Genetic Markers