title: Conformational analysis of sea cucumber (Caudina arenicola) C globin 94-97 fragment, Pro-Glu-Leu-Leu.
abstract: The tetrapeptide Pro-Glu-Leu-Leu forms the 94-97 fragment of C globin in sea cucumber. 2% Butanediol dimethacrylate-cross linked polystyrene (2% BDDMA-PS), which had been optimized, was used for the synthesis of the tetrapeptide Pro-Glu-Leu-Leu. The peptide was synthesized by using Boc-amino acid strategy. The peptide purity was checked by RP-HPLC and the peptide was characterized by (1)H NMR spectroscopy and amino acid analysis. Conformation of the peptide was studied by 1D- and 2D- homonuclear (1)H NMR, in DMSO-d6 at 300K. The conformation of the synthetic tetrapeptide (extended backbone conformation) is not in agreement with that in C globin.
authors.affiliation: School of Biosciences, Mahatma Gandhi University, Kottayam-686 560, Kerala, India.
authors.forename: P N
authors.initials: PN
authors.lastname: Sunil Kumar
authors.forename: K S
authors.initials: KS
authors.lastname: Devaky
authors.forename: C
authors.initials: C
authors.lastname: Sadasivan
authors.forename: M
authors.initials: M
authors.lastname: Haridas
mesh_terms: D000818:Animals; D005914:Globins / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D012616:Sea Cucumbers / Q000737:chemistry*
chemical_list: D010455:Peptides; D005914:Globins