Tightly regulated gene expression system in Salmonella enterica serovar Typhimurium.
A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P(BAD) control element into the bacterial chromosome allows dynamic control of T7 promoter-driven RNA transcription.
McKinney
Jeffrey
J
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
Guerrier-Takada
Cecilia
C
Galán
Jorge
J
Altman
Sidney
S
D051777:AraC Transcription Factor; D001089:Arabinose / Q000378:metabolism; D001426:Bacterial Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial; D012097:Repressor Proteins / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics*; D014157:Transcription Factors; D014158:Transcription, Genetic; D014764:Viral Proteins
D051777:AraC Transcription Factor; D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D001089:Arabinose; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D005947:Glucose