The role of T cells in protection by an inactivated infectious bursal disease virus vaccine.
The current belief is that the humoral immune response plays the principal role in defense against virulent infectious bursal disease virus (IBDV). In this study we used a model, in which chickens were compromised in functional T cells by neonatal thymectomy and Cyclosporin A (TxCsA) treatment, to demonstrate the role of T cells in protective immunity against IBDV. We demonstrated that T cells were necessary to achieve full protection against virulent IBDV. When T cell compromised TxCsA-treated chickens were vaccinated with an inactivated IBDV (iIBDV) vaccine, 91% were not protected against IBDV challenge in comparison to T cell-intact chickens, which had a protection rate of 91%. The iIBDV vaccine induced virus neutralizing (VN) and ELISA antibodies, respectively, in 65 and 5% of TxCsA-treated, and in 100 and 58% of T cell-intact birds. These observations provide evidence that the stimulation of T helper cells is needed for the production of protective antibody levels in iIBDV-vaccinated chickens. Passive administration of VN anti-IBDV antibodies inducing a circulating antibody level of log(2)8 in chickens revealed that the levels of antibodies that protected T cell-intact chickens against virulent IBDV challenge were not protective for TxCsA chickens. These results indicated that antibody alone was not adequate in inducing protection against IBDV in chickens and that T cell-involvement was critical for protection. We propose that the inability of iIBDV to protect TxCsA chickens was due to compromised T cell immunity, functional T helper cells and most likely also cytotoxic T cells are needed in iIBDV vaccine protection.
Rautenschlein
Silke
S
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA. silke.rautenschlien@tiho-hannover.de
Yeh
H-Y
HY
Sharma
J M
JM
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D018175:Birnaviridae Infections / Q000276:immunology* / Q000517:prevention & control* / Q000662:veterinary; D002645:Chickens / Q000276:immunology* / Q000821:virology*; D003520:Cyclophosphamide / Q000276:immunology / Q000494:pharmacology; D016572:Cyclosporine / Q000276:immunology / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000276:immunology / Q000494:pharmacology; D007243:Infectious bursal disease virus / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D013934:Thymectomy; D013950:Thymus Gland / Q000276:immunology; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D007166:Immunosuppressive Agents; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D016572:Cyclosporine; D003520:Cyclophosphamide