[Effect of probe purification on the reutilization of gene chip].
OBJECTIVE
To investigate the effect of probe purity on the reutilization of gene chips.


METHODS
Purified and unpurified probes were respectively hybridized with the gene chip and after being scanned with Scanarry lite, a laser scanning device, the gene chip was treated with stripping solution to wash off the probes. After another round of scanning under the same condition to obtain the images, the gene chip was recycled for another hybridization.


RESULTS
Hybridization of unpurified probes resulted in high background noise and obscure positive signals, which were present even after wash with stripping solution for 4 times. Hybridization using purified probes, in contrast, presented low background noise that was completely eliminated after the gene chip was washed twice before it was used again.


CONCLUSIONS
The purity of probes is of great importance to the recycling of the gene chips, and hybridization with unpurified probes renders the chips impossible for reutilization.
Zhang
Bao
B
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China. zhangb@fimmu.edu.cn
Ma
Wen-Li
WL
Shi
Rong
R
Wu
Qing-Hua
QH
Zheng
Wen-Ling
WL
D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000592:standards; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary