Genetic alterations in adenoma-carcinoma sequencing of intraductal papillary-mucinous neoplasm of the pancreas.
We investigated the adenoma-carcinoma sequence in intraductal papillary-mucinous neoplasm from the aspect of genetic changes. The formalin-fixed paraffin-embedded tumors and surrounding normal pancreatic tissues from patients with 16 intraductal papillary-mucinous adenoma of the pancreas (IPMA) and 10 intraductal papillary-mucinous carcinoma of the pancreas (IPMC) were provided for DNA extraction after microdissection. SSCP-DNA sequencing analysis demonstrated K-ras mutations at codon 12 in 75% of IPMA and 70% of IPMC, while those at codon 13 were observed neither in IPMA nor IPMC. There were no characteristic K-ras mutation types in IPMA and IPMC and no significant differences in incidence of K-ras mutations between the two categories. The frequencies of p53 mutations analyzed by SSCP-DNA sequencing were not high in IPMA (18.8%) and IPMC (30%), showing no significant difference between them. LOHs of APC in IPMA and IPMC were infrequent (6.3 and 20%, respectively) and showed no significant difference in incidence between the two categories. The LOH frequencies of DCC in IPMA and IPMC were 31.3 and 40%, respectively, and were not statistically different from each other. Taken together, genetic changes such as K-ras, p53, APC and DCC mutations may not be associated with adenoma-carcinoma sequence in intraductal papillary-mucinous neoplasm of pancreas.
Nakata
Bunzo
B
Department of Surgical Oncology (First Department of Surgery), Osaka City University Graduate Shool of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka 545-8585, Japan. bunzo@med.osaka-cu.ac.jp
Yashiro
Masakazu
M
Nishioka
Nobuaki
N
Aya
Makoto
M
Yamada
Shinobu
S
Takenaka
Chiemi
C
Ohira
Masaichi
M
Ishikawa
Tetsuro
T
Nishino
Hiroji
H
Wakasa
Kenichi
K
Hirakawa
Kosei
K
D002288:Adenocarcinoma, Mucinous / Q000235:genetics*; D000236:Adenoma / Q000235:genetics*; D000368:Aged; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics*; D002291:Carcinoma, Papillary / Q000235:genetics*; D005260:Female; D017491:Genes, APC; D016163:Genes, DCC; D016158:Genes, p53; D011905:Genes, ras; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010190:Pancreatic Neoplasms / Q000235:genetics*