Influence of nimodipine on elimination of soman in rabbit blood and distribution of [3H]soman in mice.
OBJECTIVE
To investigate the effect of nimodipine on the elimination of soman in rabbit blood and distribution of [3H]soman in mice.


METHODS
Chirasil capillary gas chromatographic analysis method with large volume injections was used to determine the concentration of C(+/-)P(-)soman in rabbit blood. [3H]soman trace method was used to study the effect of nimodipine on soman distribution in mice.


RESULTS
Nimodipine (10 mg/kg, ip, 1 h pre-treated) could significantly reduce the concentration of C(+/-)P(-)soman in rabbit blood from (54+/-13) to (19+/-12) microg/L blood at 15 s after soman injection (43.2 microg/kg, iv). Nimodipine could increase clearance rate [CL(S)] from (20.8+/-1.5) to (31+/-11) mL/kg/s and reduce AUC of C(+/-)P(-)soman from (2.08+/-0.15) to (1.6+/-0.4) mg/s. Nimodipine (10 mg/kg, ip, 1 h pre-treated) treatment could significantly reduce the distribution amount of bound [3H]soman in plasma, brain, lung, and liver, moreover increased the distribution amount of bound [3H]soman in small intestine during 0-120 min after mice received [3H]soman (0.544 GBq*119 microg/kg, sc) compared to soman control group.


CONCLUSIONS
Nimodipine might alter the distribution of soman and reduce the initial concentration of soman in rabbit blood, then accelerated the metabolic detoxication of soman.
Li
Jin-Tong
JT
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. lijintong@163.net.
Ruan
Jin-Xiu
JX
Yuan
Shu-Lan
SL
Zhang
Zhen-Qing
ZQ
Song
Zheng-Yan
ZY
Wang
Xiao-Ying
XY
D000818:Animals; D019540:Area Under Curve; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002849:Chromatography, Gas; D004347:Drug Interactions; D005260:Female; D008658:Inactivation, Metabolic; D008297:Male; D051379:Mice; D009553:Nimodipine / Q000494:pharmacology*; D011817:Rabbits; D012999:Soman / Q000097:blood / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D002121:Calcium Channel Blockers; D009553:Nimodipine; D012999:Soman