Laser-induced multidimensional fluorescence spectroscopy in Shpol'skii matrixes for the analysis of polycyclic aromatic hydrocarbons in HPLC fractions and complex environmental extracts.
Fluorescence measurements are easily made with the aid of a cryogenic fiber optic probe. Emission wavelength time matrixes, excitation emission matrixes, and time-resolved excitation emission matrixes are rapidly collected with a pulsed tunable dye laser, a spectrograph, and an intensified charged-coupled device. Compound identification is based on spectral and lifetime information. The potential of this approach for supporting high-performance liquid chromatography analysis and for the direct determination of benzo[a]pyrene without previous separation is demonstrated.
Bystol
Adam J
AJ
Thorstenson
Tim
T
Campiglia
Andres D
AD
D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis*; D005336:Fiber Optic Technology; D007834:Lasers; D055100:Optical Fibers; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D004785:Environmental Pollutants; D011084:Polycyclic Aromatic Hydrocarbons