Production of alpha-amylase with Aspergillus oryzae on spent brewing grain by solid substrate fermentation.
Ten Aspergillus oryzae strains were screened in solid substrate fermentation for alpha-amylase production on spent brewing grain (SBG) and on corn fiber. SBG proved to be a better substrate for enzyme production than corn fiber. A Plackett-Burman experimental design was used to optimize the medium composition for the best strain. Solid substrate fermentation on optimized medium with A. oryzae NRRL 1808 (=ATCC 12892) strain in stationary 500-mL Erlenmeyer flask culture yielded 4519 U of alpha-amylase/g of dry matter substrate in 3 d. The whole solid substrate fermentation material (crude enzyme, in situ enzyme) may be considered a cheap biocatalytic material for animal feed rations and for bioalcohol production from starchy materials.
Bogar
B
B
Department of Agricultural Chemical Technology, Budapest University of Technology and Economics, Hungary.
Szakacs
G
G
Tengerdy
R P
RP
Linden
J C
JC
Pandey
A
A
D001236:Aspergillus oryzae / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D002523:Edible Grain / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D012044:Regression Analysis; D013379:Substrate Specificity; D014867:Water / Q000032:analysis; D003313:Zea mays / Q000378:metabolism; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000378:metabolism
D003470:Culture Media; D014867:Water; D000516:alpha-Amylases