DNase I sensitive site in the core region of the human beta-globin origin of replication.
HeLa cells were synchronized at late G1, early S, and late S phase of the cell cycle by nocodazole treatment. The cells were permeabilized with Triton X-100, digested with DNAse I, and extracted with 0.2 M ammonium sulfate to remove the digested chromatin. DNA was isolated from the residual chromatin attached to the nuclear matrix, digested with Hind III, and subjected to hybridization with [(32)P] labeled probe located upstream of the core region of the human beta-globin replication origin. The hybridization pattern revealed the existence of a DNase I sensitive site in the core region of the beta-globin replicator. The results suggest that association with the nuclear matrix induce alteration in the chromatin structure of the origin of replication that represents a more open chromatin configuration.
Djeliova
Vera
V
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Russev
George
G
Anachkova
Boyka
B
D001665:Binding Sites; D002453:Cell Cycle; D002463:Cell Membrane Permeability; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D003850:Deoxyribonuclease I / Q000378:metabolism*; D005914:Globins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D015739:Nocodazole / Q000494:pharmacology; D015530:Nuclear Matrix / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D018741:Replication Origin / Q000235:genetics*; D015183:Restriction Mapping
D005914:Globins; D004247:DNA; D003850:Deoxyribonuclease I; D015739:Nocodazole