A nonradioactive assay method for determination of enzymatic activity of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco).
A sensitive and nonradioactive assay method for activity determination of Rubisco is described. The method is based on thin-layer chromatographic separation of 3-phosphoglycerate (3-PGA) and D-ribulose-1,5-bisphosphate (RuBP). This assay method allows the quantitative determination of Rubisco activity. Rates of carbon dioxide fixation on RuBP determined by this method were comparable to those obtained independently by other methods. This assay method is reproducible and relatively free from interference.
Chakrabarti
Subhra
S
Environmental Biotechnology Division, ABRD Company LLC, 1555 Wood Road, Cleveland, OH 44121, USA. sbc-abrd@usa.net
Bhattacharya
Sumana
S
Bhattacharya
Sanjoy K
SK
D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer / Q000379:methods*; D004789:Enzyme Activation; D005988:Glyceric Acids / Q000737:chemistry / Q000302:isolation & purification; D007700:Kinetics; D015203:Reproducibility of Results; D012273:Ribulose-Bisphosphate Carboxylase / Q000032:analysis* / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D012274:Ribulosephosphates / Q000737:chemistry / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013045:Species Specificity; D018724:Spinacia oleracea / Q000737:chemistry / Q000201:enzymology; D014908:Triticum / Q000737:chemistry / Q000201:enzymology; D003313:Zea mays / Q000737:chemistry / Q000201:enzymology
D002250:Carbon Radioisotopes; D005988:Glyceric Acids; D012274:Ribulosephosphates; C001933:ribulose-1,5 diphosphate; C005156:3-phosphoglycerate; D012273:Ribulose-Bisphosphate Carboxylase