Crystallization and preliminary X-ray diffraction analysis of FKBP12 complexed with a novel neurotrophic ligand.
A novel neurotrophic ligand, (3R)-4-(p-Toluenesulfonyl)-1,4-thiazane-3-carboxylic acid-L-Leucine ethyl ester, has been complexed with FKBP12 and crystallized using the hanging-drop vapor-diffusion method. Crystals belong to P2(1) space group, with unit cell parameters a=41.2, b=29.6, c=41.5 A, beta=114.0 degrees. The crystals diffract to 1.8 A resolution limit.
Li
Pengyun
P
Laboratory of Structural Biology, Department of Biological Science and Technology & MOE Laboratory of Protein Science, Tsinghua University, Beijing 100084, China.
Wang
Liwei
L
Ding
Yi
Y
Wu
Beili
B
Shu
Cuiling
C
Nie
Aihua
A
Li
Song
S
Shen
Beifen
B
Rao
Zihe
Z
D018360:Crystallography, X-Ray; D006801:Humans; D008024:Ligands; D022061:Tacrolimus Binding Protein 1A / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D008024:Ligands; D022061:Tacrolimus Binding Protein 1A