[Dendritic cells transfected with carcinoembryonic antigen-vaccinia recombinant virus induces CEA-specific immunity mediated by cytotoxic T lymphocytes in vitro].
OBJECTIVE
To observe whether dendritic cells (DCs) transfected with carcinoembryonic antigen (CEA)-vaccinia recombinant virus (rV-CEA) induces cytotoxic T lymphocyte-mediated CEA-specific immunity in vitro.


METHODS
DCs derived from peripheral blood monocytes were transfected with rV-CEA and then cocultured with autologous T cells. The proliferation of the induced T cells and their cytotoxic activity against CEA-secreting tumor cells were assessed and compared with those of T cells induced by DCs without rV-CEA transfection.


RESULTS
T cells induced by DCs transfected with rV-CEA showed specific cytotoxicity against CEA-secreting tumor cells.


CONCLUSIONS
DCs transfected with rV-CEA can elicit activation of CEA-specific T cells.
Wu
Jun
J
Department of Oncology, General Hospital of Guangzhou Command, Guangzhou 510010, China.
Wang
Xiao-Huai
XH
Yang
Tai-Cheng
TC
Xian
Jiang
J
Zheng
Wen-Ling
WL
D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000276:immunology*; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D014616:Vaccinia virus / Q000235:genetics
D002272:Carcinoembryonic Antigen; D011993:Recombinant Fusion Proteins