title: Natural killer cell activation after infection with lactate dehydrogenase-elevating virus.
abstract: Early after infection, lactate dehydrogenase-elevating virus (LDV) alters the immune system by polyclonally activating B lymphocytes, which leads to IgG2a-restricted hypergammaglobulinaemia, and by suppressing the secretion of Th2 cytokines. Considering that these alterations may involve cells of the innate immune system and cytokines such as interferon-gamma (IFN-gamma), we analysed the effect of LDV on natural killer (NK) cells. Within a few days of infection, a strong and transient NK cell activation, characterized by enhanced IFN-gamma message expression and cytolysis, was observed. LDV triggered a large increase in serum IFN-gamma levels. Because NK cells and IFN-gamma may participate in the defence against virus infection, we analysed their possible role in the control of LDV titres with a new agglutination assay. Our results indicate that neither the activation of NK cells nor the IFN-gamma secretion affect the early and rapid virus replication that follows LDV inoculation.
authors.affiliation: Unit of Experimental Medicine1 and Unit of Cellular Genetics2, Institute for Cellular Pathology, Université Catholique de Louvain, UCL MEXP 7430, Av. Hippocrate 74, 1200 Bruxelles, Belgium.
authors.forename: Dominique
authors.initials: D
authors.lastname: Markine-Goriaynoff
authors.affiliation: Unit of Experimental Medicine1 and Unit of Cellular Genetics2, Institute for Cellular Pathology, Université Catholique de Louvain, UCL MEXP 7430, Av. Hippocrate 74, 1200 Bruxelles, Belgium.
authors.forename: Xavier
authors.initials: X
authors.lastname: Hulhoven
authors.affiliation: Unit of Experimental Medicine1 and Unit of Cellular Genetics2, Institute for Cellular Pathology, Université Catholique de Louvain, UCL MEXP 7430, Av. Hippocrate 74, 1200 Bruxelles, Belgium.
authors.forename: César L
authors.initials: CL
authors.lastname: Cambiaso
authors.affiliation: Unit of Experimental Medicine1 and Unit of Cellular Genetics2, Institute for Cellular Pathology, Université Catholique de Louvain, UCL MEXP 7430, Av. Hippocrate 74, 1200 Bruxelles, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Monteyne
authors.affiliation: Unit of Experimental Medicine1 and Unit of Cellular Genetics2, Institute for Cellular Pathology, Université Catholique de Louvain, UCL MEXP 7430, Av. Hippocrate 74, 1200 Bruxelles, Belgium.
authors.forename: Thérèse
authors.initials: T
authors.lastname: Briet
authors.affiliation: Unit of Experimental Medicine1 and Unit of Cellular Genetics2, Institute for Cellular Pathology, Université Catholique de Louvain, UCL MEXP 7430, Av. Hippocrate 74, 1200 Bruxelles, Belgium.
authors.forename: Maria-Dolores
authors.initials: MD
authors.lastname: Gonzalez
authors.affiliation: Unit of Experimental Medicine1 and Unit of Cellular Genetics2, Institute for Cellular Pathology, Université Catholique de Louvain, UCL MEXP 7430, Av. Hippocrate 74, 1200 Bruxelles, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Coulie
authors.affiliation: Unit of Experimental Medicine1 and Unit of Cellular Genetics2, Institute for Cellular Pathology, Université Catholique de Louvain, UCL MEXP 7430, Av. Hippocrate 74, 1200 Bruxelles, Belgium.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Coutelier
mesh_terms: D000818:Animals; D018174:Arterivirus Infections / Q000097:blood / Q000276:immunology* / Q000821:virology; D015415:Biomarkers; D003602:Cytotoxicity, Immunologic; D015870:Gene Expression; D039421:Integrin alpha2; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D007772:Lactate dehydrogenase-elevating virus / Q000276:immunology*; D018655:Lymphocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D018345:Mice, Knockout; D016513:Mice, SCID; D010537:Peritoneum / Q000166:cytology; D017471:Receptors, Interferon / Q000235:genetics; D013154:Spleen / Q000166:cytology; D014766:Viremia; D000096743:Interferon gamma Receptor
chemical_list: D015415:Biomarkers; D039421:Integrin alpha2; D007371:Interferon-gamma; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor