title: Influence of severe endometriosis on gene expression of vascular endothelial growth factor and interleukin-6 in granulosa cells from patients undergoing controlled ovarian hyperstimulation for in vitro fertilization-embryo transfer.
abstract: OBJECTIVE
  To evaluate how endometriosis affects expression of vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) in granulosa cells.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  IVF-ET program at Osaka Medical College.
  
  
  METHODS
  Seventeen patients with revised American Fertility Society stage IV endometriosis and 17 patients with tubal infertility and no endometriosis.
  
  
  METHODS
  Granulosa cells obtained at oocyte retrieval were examined for VEGF and IL-6 gene expression.
  
  
  METHODS
  Serum E(2) and P levels at hCG administration, number of oocytes, fertilization rate, high-quality embryo rate, and pregnancy rate, and expression of VEGF and IL-6 genes.
  
  
  RESULTS
  Total hMG and FSH levels were statistically significantly higher in patients with endometriosis; however, the number of retrieved oocytes and the fertilization rate were lower compared with patients with tubal infertility. Serum E(2) levels and expression of VEGF in patients with tubal infertility were statistically significantly higher than those in patients with endometriosis. Interleukin-6 gene expression did not differ between the groups.
  
  
  CONCLUSIONS
  In severe endometriosis, lower VEGF gene expression in granulosa cells may adversely affect oocyte development and maturation.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka Medical College, Takatsuki, Osaka, Japan. gyn028@poh.osaka-med.ac.jp
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Ueda
authors.forename: Mikio
authors.initials: M
authors.lastname: Takehara
authors.forename: Hikari
authors.initials: H
authors.lastname: Yamashita
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Yao-Ching
authors.initials: YC
authors.lastname: Hung
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Terai
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueki
mesh_terms: D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D004624:Embryo Transfer; D004715:Endometriosis / Q000150:complications / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000235:genetics*; D004958:Estradiol / Q000097:blood; D005184:Fallopian Tube Diseases / Q000150:complications; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000097:blood; D015870:Gene Expression; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000628:therapy; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D008222:Lymphokines / Q000235:genetics*; D008596:Menotropins / Q000097:blood; D009865:Oocytes / Q000502:physiology; D010062:Ovulation Induction; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011446:Prospective Studies; D027724:Reproductive Techniques, Assisted; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D006063:Chorionic Gonadotropin; D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D015850:Interleukin-6; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D011374:Progesterone; D004958:Estradiol; D008596:Menotropins; D005640:Follicle Stimulating Hormone