title: Effect of somatostatin on rabbit isolated coronary arteries.
abstract: Somatostatin analogues are capable of inhibiting vascular smooth muscle and endothelial cell proliferation. However, little is known about the effect of somatostatin on vascular responses in endothelium-denuded coronary arteries in vitro. The aim of this work was to determine whether or not somatostatin prevented the contractile response induced by 5-hydroxytryptamine and acetylcholine in endothelium-denuded rabbit coronary arteries. Somatostatin attenuated the contraction produced by 5-hydroxytryptamine in both proximal (PC) and distal coronary (DC) arteries (contraction induced by 10(-4) M 5-hydroxytryptamine was inhibited by 10(-6) M somatostatin by 90.8 +/- 11.0% (P < 0.001, n = 9) and by 46.2 +/- 14.0% (P < 0.05, n = 9) in DC and PC, respectively), but concentration-dependently decreased the contraction induced by U46619 (11alpha-epoxy-methanoprostaglandin F2alpha) only in PC arteries, suggesting that the response of PC and DC arteries to somatostatin were qualitatively different. Furthermore, we suggest that somatostatin may enhance acetylcholine-induced relaxation by combination of increasing endothelium-dependent relaxation (by a NO-dependent mechanism) and blocking contraction at the muscle level.
authors.affiliation: Department of Pharmacology, School of Medicine, Universidad Complutense, Madrid, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Ruiz
authors.forename: Eugenia
authors.initials: E
authors.lastname: Padilla
authors.forename: Teresa
authors.initials: T
authors.lastname: Tejerina
mesh_terms: D000818:Animals; D001158:Arteries / Q000187:drug effects / Q000502:physiology; D003331:Coronary Vessels / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits; D013004:Somatostatin / Q000494:pharmacology*; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology
chemical_list: D014662:Vasoconstrictor Agents; D013004:Somatostatin