NF-kappaB activation in cerebrospinal fluid cells from patients with meningitis.
We examined whether or not NF-kappaB, a factor that regulates expression of the genes that code for pro-inflammatory cytokines, is activated in cerebrospinal fluid (CSF) cells to investigate the production of pro-inflammatory cytokines by CSF cells in patients with meningitis. Western blotting demonstrated that NF-kappaB was more activated in CSF cells of patients with bacterial meningitis than in those of patients with aseptic meningitis. NF-kappaB was hardly activated in carcinomatous meningitis. The NF-kappaB activation in CSF cells of patients with meningitis tended to be correlated with the CSF interleukin-6 concentration. Our data suggested that CSF cells produce pro-inflammatory cytokines through NF-kappaB activation in meningitis, and that increased NF-kappaB activation in CSF cells indicate infectious meningitis rather than carcinomatous meningitis.
Ichiyama
Takashi
T
Department of Pediatrics, Yamaguchi University School of Medicine, Ube, Japan. ichiyama@yamaguchi-u.ac.jp
Isumi
Hiroshi
H
Yoshitomi
Tomomi
T
Nishikawa
Miki
M
Matsubara
Tomoyo
T
Furukawa
Susumu
S
D002555:Cerebrospinal Fluid / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis / Q000134:cerebrospinal fluid* / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015850:Interleukin-6 / Q000134:cerebrospinal fluid; D008297:Male; D008577:Meningeal Neoplasms / Q000134:cerebrospinal fluid / Q000556:secondary; D008581:Meningitis / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000276:immunology*; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000276:immunology; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000276:immunology; D016328:NF-kappa B / Q000134:cerebrospinal fluid* / Q000276:immunology; D009442:Neurilemmoma / Q000134:cerebrospinal fluid / Q000556:secondary
D016207:Cytokines; D015850:Interleukin-6; D016328:NF-kappa B