title: Parathyroid hormone-related protein (PTHrP) produced by dog lymphoma cells.
abstract: Parathyroid hormone-related protein (PTHrP) was investigated in a canine lymphoma case with hypercalcemia by means of immunoradiomentric assay (IRMA) and molecular analysis. The plasma calcium level of the patient dog was 13.7 mg/dl. The PTHrP concentration examined by IRMA was 6.1 pmol/L in the plasma sample from the dog, but it was undetectable (< 1.1 pmol/L) in plasma samples from 4 lymphoma cases without hypercalcemia or 5 normal dogs. The PTHrP concentration examined in the culture supernatant of the lymphoma cells from this case was 1.3 pmol/L, whereas those of the lymphoma cells from a lymphoma case without hypercalcemia was undetectable. PTHrP mRNA was clearly detected not only in the lymphoma cells from this dog with hypercalcemia but also in lymphoma cells from 4 lymphoma cases without hypercalcemia and 2 canine lymphoma cell lines.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kubota
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Takuya
authors.initials: T
authors.lastname: Mizuno
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hisasue
authors.forename: Peter F
authors.initials: PF
authors.lastname: Moore
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Watari
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D002118:Calcium / Q000097:blood; D002460:Cell Line; D004283:Dog Diseases / Q000097:blood / Q000235:genetics*; D004285:Dogs; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006934:Hypercalcemia / Q000097:blood / Q000150:complications / Q000235:genetics; D008223:Lymphoma / Q000150:complications / Q000235:genetics* / Q000662:veterinary*; D008297:Male; D044162:Parathyroid Hormone-Related Protein; D036361:Peptide Hormones / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D044162:Parathyroid Hormone-Related Protein; D036361:Peptide Hormones; D012333:RNA, Messenger; D002118:Calcium