title: Expression profiling of the whole Arabidopsis shaggy-like kinase multigene family by real-time reverse transcriptase-polymerase chain reaction.
abstract: The recent publication of the complete sequence of the Arabidopsis genome allowed us to identify and characterize the last two members of the SHAGGY-like kinase (AtSK) gene family. As a result, the study of the overall spatio-temporal organization of the whole AtSK family in Arabidopsis has become an achievable and necessary aim to understand the role of each SHAGGY-like kinase during plant development. An analysis of the transcript level of the 10 members of the family has been performed using the technique of real-time quantitative reverse transcriptase-polymerase chain reaction. Transcript levels in several organs, under different growth conditions, were analyzed. To calibrate the results obtained, a number of other genes, such as those coding for the two MAP3Kepsilons and the two MAP4Kalphas, as well as the stress response marker RD29A; the small subunit of the Rubisco photosynthetic enzyme Ats1A; the MEDEA chromatin remodeling factor; and the SCARECROW, ASYMMETRIC LEAVES 1, and SUPERMAN transcription factors all involved in key steps of plant development were used. The analysis of our data revealed that eight of the 10 genes of the AtSK family displayed a pseudo-constitutive expression pattern at the organ level. Conversely, AtSK13 responded to osmotic changes and saline treatment, whereas AtSK31 was flower specific and responded to osmotic changes and darkness.
authors.affiliation: Laboratoire de Biologie du Développement des Plantes, Bâtiment 630, Unité Mixte de Recherche-Centre National de la Recherche Scientifique 8618, Université Paris-Sud (XI), 91405 Orsay cedex, France. charrier@ibp.u-psud.fr
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Charrier
authors.forename: Anthony
authors.initials: A
authors.lastname: Champion
authors.forename: Yves
authors.initials: Y
authors.lastname: Henry
authors.forename: Martin
authors.initials: M
authors.lastname: Kreis
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D038362:Glycogen Synthase Kinase 3 / Q000031:analogs & derivatives* / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D008027:Light; D009997:Osmotic Pressure / Q000187:drug effects; D010802:Phylogeny; D011092:Polyethylene Glycols / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012965:Sodium Chloride / Q000494:pharmacology; D013314:Stress, Mechanical; D014157:Transcription Factors / Q000235:genetics; D014867:Water / Q000494:pharmacology / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D014157:Transcription Factors; D014867:Water; D011092:Polyethylene Glycols; D012965:Sodium Chloride; C085936:ATSK11 protein, Arabidopsis; D038362:Glycogen Synthase Kinase 3