Evidence for the hydrolysis of topical applied liposomal lipids in human stratum corneum.
Liposomes were incubated with an extract of human plantar stratum corneum. The liposomal lipids were hydrolysed, if composed of soy-bean phosphatidylcholine or phosphatidylglycerol. Rigid lipids were not degraded. The temperature optimum of the hydrolysis was between 30-35degC. CaCl enhanced, while EDTA reduced the rate of hydrolysis, indicating that the hydrolysis is due to a phospholipase A(2).
Zellmer
Sebastian
S
Labsoft Diagnostics AG, Robert-Franz-Ring 21, 06108 Halle, Germany. zellmer@labsoft.de
D000287:Administration, Topical; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008081:Liposomes / Q000008:administration & dosage* / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000008:administration & dosage / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000008:administration & dosage / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*
D008081:Liposomes; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010741:Phospholipases A