title: [Application of multiplex PCR in genotyping of hepatitis B virus prevailing in Guangdong Province of China].
abstract: OBJECTIVE
  To establish a convenient method for the genotyping of hepatitis B virus (HBV) using multiplex PCR.
  
  
  METHODS
  Based on the alignment of 114 complete nucleotide sequences of HBV DNA belonging to different genotypes, acquired from the GenBank, genotype-specific sequences were identified according to which 6 pairs of primers were designed corresponding to each genotype. Subsequent genotyping of HBV was performed using these primers that were added, either alone or in conjunction with others, into a multiplex PCR reaction tube, and HBV genotype was determined according to the length of amplified DNA.
  
  
  RESULTS
  The genotyping result of multiplex PCR was consistent with that produced by PCR- restriction fragment length polymorphism as established by Lindh. We found in this study that among the HBV carriers in the vicinities Guangzhou of City, about 45% belonged to B genotype, 38.75% to C genotype and 16.75% to D genotype.
  
  
  CONCLUSIONS
  This multiplex PCR method is simple, convenient and more differential.
authors.affiliation: Department of Infectious Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. yangjie@fimmu.edu.cn
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Lin
authors.initials: L
authors.lastname: Dai
authors.forename: Ya-Bing
authors.initials: YB
authors.lastname: Guo
authors.forename: Shou-Chang
authors.initials: SC
authors.lastname: Yang
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: Kang-Xian
authors.initials: KX
authors.lastname: Luo
mesh_terms: D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D005838:Genotype; D006509:Hepatitis B / Q000453:epidemiology / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral