The value of tissue imprint hybridization for rapid detection of infectious bursal disease virus from field outbreaks.
The use of a peroxidase labelled PCR generated probe followed by enhanced chemiluminescence hybridization assay detected infectious bursal disease virus directly from bursal imprints on a nylon membrane. Tissue imprint hybridization proved to be a simple, rapid and safe means of detecting IBD virus for screening large numbers of field samples. The PCR generated probe was highly specific for IBD virus and did not hybridize with cellular nucleic acids in control imprints. Tissue imprint hybridization was found to be a more sensitive method than conventional antigen detection assays.
Parthiban
M
M
Department of Animal Biotechnology, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India.
Thiagarajan
V
V
D000818:Animals; D018175:Birnaviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D002060:Bursa of Fabricius / Q000821:virology; D002645:Chickens; D007194:India; D007243:Infectious bursal disease virus / Q000235:genetics / Q000302:isolation & purification*; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011201:Poultry Diseases / Q000175:diagnosis / Q000821:virology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D015678:Viral Structural Proteins / Q000235:genetics
D012367:RNA, Viral; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins