title: [Cytoskeletal mechanisms of heterocellular contact formation].
abstract: We studied the interaction of two main cell types, epitheliocytes and fibroblasts, in a mixed culture. Heterotypic cells had a different cytoskeleton organization and expressed different cell adhesion molecules, cadherins. In spite of this, when the cells contacted in the mixed cultures, a heterophilic contact was formed and the actin cytoskeleton of an epitheliocyte at the site of contact was reorganized: the marginal actin bundle was decomposed and actin structures were formed in its place, that were typical for the fibroblast lamella. No changes were observed in the actin organization of the fibroblast. In architecture, the heterophilic adhesion contacts resembled the contacts between fibroblasts. Both heterophilic and homophilic contacts were transient, rather than constant structures. The formation of heterophilic contacts in mixed cultures can serve as a model of formation of a tissue system consisting of epithelium and mesenchyme.
authors.affiliation: Belozersky Research Institute of Physicochemical Biology, Moscow State University, Vorob'evy gory, Moscow, 119992 Russia.
authors.forename: E K
authors.initials: EK
authors.lastname: Fetisova
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Ivanova
authors.forename: T
authors.initials: T
authors.lastname: Omelchenko
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Vasil'ev
mesh_terms: D000199:Actins / Q000502:physiology; D000818:Animals; D015820:Cadherins / Q000502:physiology; D002460:Cell Line; D018920:Coculture Techniques; D003599:Cytoskeleton / Q000502:physiology / Q000648:ultrastructure; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007365:Intercellular Junctions / Q000502:physiology / Q000648:ultrastructure; D051381:Rats
chemical_list: D000199:Actins; D015820:Cadherins