Drug delivery in pluronic micelles: effect of high-frequency ultrasound on drug release from micelles and intracellular uptake.
The effect of high-frequency ultrasound on doxorubicin (DOX) release from Pluronic micelles and intracellular DOX uptake was studied for promyelocytic leukemia HL-60 cells, ovarian carcinoma drug-sensitive and multidrug-resistant (MDR) cells (A2780 and A2780/ADR, respectively), and breast cancer MCF-7 cells. Cavitation events initiated by high-frequency ultrasound were recorded by radical trapping. The onset of transient cavitation and DOX release from micelles were observed at much higher power densities than at low-frequency ultrasound (20-100 kHz). Even a short (15-30 s) exposure to high-frequency ultrasound significantly enhanced the intracellular DOX uptake from PBS, RPMI 1640, and Pluronic micelles. The mechanisms of the observed effects are discussed.
Marin
Alexandre
A
Department of Bioengineering, 20 S. 2030 E. Room 108, University of Utah, Salt Lake City 84112, USA.
Sun
Hao
H
Husseini
Ghaleb A
GA
Pitt
William G
WG
Christensen
Douglas A
DA
Rapoport
Natalya Y
NY
D000465:Algorithms; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D016503:Drug Delivery Systems; D004578:Electron Spin Resonance Spectroscopy; D005079:Excipients; D005260:Female; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism; D008823:Micelles; D010051:Ovarian Neoplasms / Q000378:metabolism; D020442:Poloxamer / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D014465:Ultrasonics
D000903:Antibiotics, Antineoplastic; D005079:Excipients; D008823:Micelles; D020442:Poloxamer; D004317:Doxorubicin