Mutations in ribosomal protein L16 and in 23S rRNA in Enterococcus strains for which evernimicin MICs differ.
Mutations in ribosomal protein L16 and in 23S rRNA were investigated in 22 Enterococcus strains of different species and for which the MICs of evernimicin differ (MICs, 0.023 to 16 micro g/ml). Amino acid changes (Arg56His, Ile52Thr, or Arg51His) in protein L16 were found in seven strains, and a nucleotide G2535A mutation in 23S rRNA was found in 1 strain among 13 for which the MICs are > or =1 micro g/ml.
Zarazaga
Myriam
M
Area de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.
Tenorio
Carmen
C
Del Campo
Rosa
R
Ruiz-Larrea
Fernanda
F
Torres
Carmen
C
D019943:Amino Acid Substitution; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D012338:RNA, Ribosomal, 23S; D012269:Ribosomal Proteins; C027937:ribosomal protein L16; C001202:evernimicin