Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion.
Obligate intracellular apicomplexan parasites rely on gliding motion powered by their actomyosin system to disperse throughout tissues and to penetrate host cells. Toxoplasma gondii myosin A has been implicated in this process, but direct proof has been lacking. We designed a genetic screen to generate a tetracycline-inducible transactivator system in T. gondii. The MyoA gene was disrupted in the presence of a second regulatable copy of MyoA. Conditional removal of this myosin caused severe impairment in host cell invasion and parasite spreading in cultured cells, and unambiguously established the pathogenic function of this motor in an animal model.
Meissner
Markus
M
Department of Biological Sciences, Imperial College of Science, Technology and Medicine, Imperial College Road, London SW7 2AZ, UK.
Schlüter
Dirk
D
Soldati
Dominique
D
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D005089:Exocytosis; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D009068:Movement; D024507:Nonmuscle Myosin Type IIA / Q000235:genetics / Q000502:physiology*; D015388:Organelles / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000502:physiology; D013752:Tetracycline / Q000494:pharmacology; D014122:Toxoplasma / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology*; D014124:Toxoplasmosis, Animal / Q000469:parasitology*; D015534:Trans-Activators / Q000378:metabolism; D014162:Transfection; D019076:Transgenes; D014774:Virulence; D037521:Virulence Factors / Q000502:physiology*
D015800:Protozoan Proteins; D015534:Trans-Activators; D037521:Virulence Factors; D000001:Calcimycin; D024507:Nonmuscle Myosin Type IIA; D013752:Tetracycline; D002118:Calcium