title: Protective effect of the phenolic fraction from virgin olive oils against oxidative stress in human cells.
abstract: This paper reports the protective effect of the phenolic fraction extracted from extra virgin olive oils (OOPEs) against the cytotoxic effects of reactive oxygen species in human erythrocytes and Caco-2 cells, employed as model systems. Pretreatment of cells with various OOPEs, indeed, provides a remarkable protection against oxidative damages: this effect was strictly dependent on the o-diphenolic content of the extracts. Moreover, the protective effects observable in cellular systems were compared with in vitro antioxidant properties, measured by using the FRAP (ferric reducing/antioxidant power) assay; the reducing ability of OOPEs strictly parallels their o-phenolic content. The linear relationship demonstrated between biological effects and antioxidant capacity measured by the FRAP assay allows us to propose the use of this rapid colorimetric method in assessing and certifying the antioxidant power of extra virgin olive oil.
authors.affiliation: Department of Biochemistry and Biophysics F. Cedrangolo, Medical School, Second University of Naples, Naples, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Manna
authors.forename: Stefania
authors.initials: S
authors.lastname: D'Angelo
authors.forename: Valentina
authors.initials: V
authors.lastname: Migliardi
authors.forename: Evelina
authors.initials: E
authors.lastname: Loffredi
authors.forename: Orazio
authors.initials: O
authors.lastname: Mazzoni
authors.forename: Patrizia
authors.initials: P
authors.lastname: Morrica
authors.forename: Patrizia
authors.initials: P
authors.lastname: Galletti
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Zappia
mesh_terms: D018938:Caco-2 Cells; D038441:Diet, Mediterranean; D004912:Erythrocytes; D006801:Humans; D000069463:Olive Oil; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry*; D017382:Reactive Oxygen Species
chemical_list: D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D017382:Reactive Oxygen Species