Design and synthesis of activity probes for glycosidases.
[structure: see text] A new synthetic route was developed for the preparation of activity probe 1 for beta-glucosidase in this study. The key glycosidation step begins with benzyl p-hydroxyphenylacetate. Benzylic functionalization for the construction of the trapping device was achieved at later stages. Probe 1 was shown to be able to label the target enzyme. This cassette-like design offers great flexibility for future alterations. It would allow the synthetic scheme to expand to other glycosidase probes with different linker/reporter combinations.
Tsai
Charng-Sheng
CS
Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
Li
Yaw-Kuen
YK
Lo
Lee-Chiang
LC
D004591:Electrophoresis, Polyacrylamide Gel; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D001617:beta-Glucosidase / Q000378:metabolism*
D015335:Molecular Probes; D001617:beta-Glucosidase