Expression of SHV-2 beta-lactamase and of reduced amounts of OmpK36 porin in Klebsiella pneumoniae results in increased resistance to cephalosporins and carbapenems.
A Klebsiella pneumoniae clinical isolate was resistant to cefoxitin, cefotaxime, ceftazidime, ceftazidime-clavulanate, piperacillin-tazobactam (MICs, >256 micro g/ml in all cases), and meropenem (MIC, 16 micro g/ml) and was intermediate to imipenem (MIC, 8 micro g/ml). Decreased expression of the OmpK36 porin and expression of an SHV-2 beta-lactamase contributed to the observed resistance to these beta-lactam-containing agents.
Crowley
Brendan
B
Public Health Laboratory, University Hospital Aintree, Liverpool L9 7AL, United Kingdom.
Benedí
Vicente J
VJ
Doménech-Sánchez
Antonio
A
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001426:Bacterial Proteins; D015780:Carbapenems / Q000494:pharmacology*; D018550:Cephalosporin Resistance; D002511:Cephalosporins / Q000494:pharmacology*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D018272:Porins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D015780:Carbapenems; D002511:Cephalosporins; D003470:Culture Media; C092233:OmpK36 protein, Klebsiella pneumoniae; D018272:Porins; C062760:beta-lactamase SHV-2; D001618:beta-Lactamases