title: A general synthesis of specifically deuterated nucleotides for studies of DNA and RNA.
abstract: An efficient procedure is described for the preparation of ribonucleotides and deoxyribonucleotides with deuterium incorporated at the 1', 4', or 5' position. Three intermediates-[1-2H]-D-ribose, [4-2H]-D-ribose, and [5-2H(2)]-D-ribose-were prepared by chemical synthesis and subsequently converted to ribonucleotides and deoxyribonucleotides via enzymatic reactions. Milligram quantities of the desired products were obtained with an average deuterium content of 96+/-1%.
authors.affiliation: Department of Chemistry, Boston University, Boston, MA 02215, USA.
authors.forename: Bingzi
authors.initials: B
authors.lastname: Chen
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Jamieson
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Tullius
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry*; D003854:Deoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D003903:Deuterium / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods*; D010766:Phosphorylation; D012313:RNA / Q000737:chemistry*; D016337:RNA, Catalytic / Q000378:metabolism; D012264:Ribonucleotide Reductases / Q000737:chemistry; D012265:Ribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012266:Ribose / Q000737:chemistry; D013768:Tetrahymena / Q000378:metabolism
chemical_list: D003854:Deoxyribonucleotides; D007202:Indicators and Reagents; D016337:RNA, Catalytic; D012265:Ribonucleotides; D012313:RNA; D012266:Ribose; D004247:DNA; D003903:Deuterium; D012264:Ribonucleotide Reductases