Adenoviral p53 gene therapy in head and neck squamous cell carcinoma cell lines.
We reconstructed the recombinant p53-expressing adenovirus and examined its infections and effects in head and neck squamous cell carcinoma cell lines. Eight human head and neck squamous cell carcinoma cell lines were infected by the recombinant adenovirus harboring the lacZ gene (AxCAiLacZ) or the wild-type p53 gene (AxCAip53), and the effects were investigated. The eight cell lines were successfully infected by AxCAiLacZ at a level of more than 50%. The survival of all 8 squamous cell lines were inhibited in the range from 8 to 26.7% by only one treatment of the AxCAip53 infection. This result suggested that p53 gene therapy might become a useful tool in head and neck squamous cell carcinoma treatment.
Higuchi
Yuzuru
Y
Department of Oral and Maxillofacial Radiology, Graduate School of Medicine and Dentistry, Okayama University, Okayama, Japan.
Asaumi
Jun-Ichi
J
Murakami
Jun
J
Wakasa
Toru
T
Inoue
Tetsuyoshi
T
Kuroda
Masahiro
M
Shibuya
Koichi
K
Shigehara
Hiroshi
H
Kawasaki
Shoji
S
Fukui
Kazuhiro
K
Kishi
Kanji
K
Hiraki
Yoshio
Y
D000256:Adenoviridae / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D002470:Cell Survival; D017931:DNA Primers / Q000737:chemistry; D005697:Galactosides / Q000378:metabolism; D016158:Genes, p53; D015316:Genetic Therapy; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D006801:Humans; D007211:Indoles / Q000378:metabolism; D007763:Lac Operon; D016133:Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000378:metabolism
D017931:DNA Primers; D005697:Galactosides; D007211:Indoles; D001616:beta-Galactosidase; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside