title: PCR assay for the detection and the identification of atypical canine coronavirus in dogs.
abstract: Comparative sequence analysis of the PCR products of the M gene and fragments of the pol1a and pol1b genes of canine coronavirus (CCoV) have demonstrated that two separate clusters of CCoV are present in dogs. This note describes a PCR assay to identify atypical CCoV strains with nucleotide substitutions in the M gene. A total of 177 faecal samples from dogs CCoV positive previously with the PCR assay were analysed. Sixty-two of the 177 samples were amplified with the PCR described in the present study and were thus considered atypical CCoVs. The specificity of the PCR typing assay was confirmed by sequence analysis of the PCR products.
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, Sp per Casamassima km 3, 70010 Valenzano, Bari, Italy. a.pratelli@veterinaria.uniba.it
authors.forename: Annamaria
authors.initials: A
authors.lastname: Pratelli
authors.forename: Antonella
authors.initials: A
authors.lastname: Tinelli
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Michele
authors.initials: M
authors.lastname: Camero
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.forename: Arturo
authors.initials: A
authors.lastname: Gentile
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D001483:Base Sequence; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D000086862:Coronavirus M Proteins; D017939:Coronavirus, Canine / Q000302:isolation & purification*; D004279:DNA, Viral; D004285:Dogs; D005243:Feces / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D014763:Viral Matrix Proteins / Q000235:genetics
chemical_list: D000086862:Coronavirus M Proteins; D004279:DNA, Viral; D014763:Viral Matrix Proteins