title: Prostaglandin D2 generation by rat peritoneal mast cells stimulated with Datura stramonium agglutinin and its inhibition by haptenic sugar and wheat germ agglutinin.
abstract: The production of prostaglandin D2 (PGD2) by rat peritoneal mast cells incubated with N-acetyl glucosamine (GlcNAc) oligomer-specific Datura stramonium agglutinin (DSA) for 10 min in the presence of 0.3 mM Ca2+ was examined. Previously, our group reported that the incubation of rat mast cells with DSA (5 - 100 microg/ml) under similar conditions resulted in a calcium influx and histamine release via a pertussis toxin-sensitive G-protein pathway of the mast cells, and the histamine release was inhibited by haptenic sugar chitooligosaccharides or GlcNAc-specific lectin wheat germ agglutinin (WGA) (K. Matsuda et al., Jpn J Pharmacol 66, 195 - 204 (1994)). DSA (5 - 100 microg/ml) dose-dependently stimulated the mast cells to generate PGD2. Chitooligosaccharides (1% w/v) and WGA (100 microg/ml) inhibited the production of PGD2 induced by 100 microg/ml of DSA, suggesting that the effect of DSA is sugar-specific. A prostaglandin G/H synthase inhibitor NS-398 (N-[cyclohexyloxy-4-nitrophenyl] methanesulfonamide) (10 microM) inhibited the formation of PGD2 induced by DSA (20 microg/ml). These results suggest that the binding of DSA to the corresponding sugar residues on the mast cell surface mediates the signaling of the prostaglandin G/H synthase pathway.
authors.affiliation: Department of Pharmacology, Meiji Pharmaceutical University, Tokyo, Japan. nishimura@kiko.go.jp
authors.forename: Tamiko
authors.initials: T
authors.lastname: Suzuki-Nishimura
authors.forename: Masaatsu K
authors.initials: MK
authors.lastname: Uchida
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology; D000818:Animals; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D013286:Datura stramonium; D006241:Haptens / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D009844:Oligosaccharides / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000502:physiology; D037121:Plant Lectins / Q000494:pharmacology*; D015230:Prostaglandin D2 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014909:Wheat Germ Agglutinins / Q000494:pharmacology*
chemical_list: D016861:Cyclooxygenase Inhibitors; C041384:Datura stramonium lectin; D006241:Haptens; D009844:Oligosaccharides; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins; D015230:Prostaglandin D2; D000117:Acetylglucosamine