Solid-state fermentation for production of phytase by Rhizopus oligosporus.
Solid-state fermentation of coconut oil cake has been carried out with Rhizopus oligosporus for the production of phytase. Phytase is used commercially in the animal feed industry to improve animal performance because there is a substantial and growing interest among swine and poultry producers in the application of phytase to improve the nutritional quality in animal feeds. Demonstrated benefits include improved feed yield ratios and reduction in the environmental costs associated with the disposal of animal wastes. We report the production of extracellular phytase by R. oligosporus under solid-state fermentation using coconut oil cake as substrate. Maximal enzyme production (14.29 U/g of dry substrate) occurred at pH 5.3, 30 degrees C, and 54.5% moisture content after 96 h of incubation. The addition of extra nutrients to the substrate resulted in inhibition of product formation. The results indicate the scope for production of phytase using coconut oil cake as solid substrate without additional nutrients.
Sabu
Abdulhameed
A
Biotechnology Division, Regional Research Laboratory (CSIR), Trivandrum, India.
Sarita
Saradamma
S
Pandey
Ashok
A
Bogar
Barbara
B
Szakacs
George
G
Soccol
Carlos Ricardo
CR
D010832:6-Phytase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D000074263:Coconut Oil; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D010833:Phytic Acid / Q000494:pharmacology; D010938:Plant Oils / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology*; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000032:analysis
D003470:Culture Media; D010938:Plant Oils; D014867:Water; D010833:Phytic Acid; D010832:6-Phytase; D000074263:Coconut Oil