title: Deletion polymorphism in the alpha2B-adrenergic receptor gene is associated with flow-mediated dilatation of the brachial artery.
abstract: A deletion variant of the alpha(2B)-adrenergic receptor (alpha(2B)-AR) has been associated with an increased risk of acute cardiac events in middle-aged men. Our aim was to determine the possible associations between the alpha(2B)-AR gene deletion variant and indicators of subclinical atherosclerosis in the brachial and carotid arteries. A total of 148 middle-aged men participating in an epidemiological twin study on risk factors for subclinical coronary heart disease were genotyped using PCR. Flow-mediated dilatation (FMD) of the brachial artery, carotid artery compliance and carotid intima-media thickness were measured using high-resolution ultrasound. FMD was 6.2+/-5.0% in subjects with the I/I (insertion/insertion) genotype, 5.5+/-4.1% in the I/D (insertion/deletion) group and 4.1+/-3.8% in the D/D (deletion/deletion) group ( P =0.03 for trend). In multivariate regression analysis controlling for age, presence of hypertension, smoking, use of angiotensin-converting enzyme inhibitors and plasma levels of low-density lipoprotein cholesterol and lipoprotein (a), the association between the alpha(2B)-AR genotype and FMD remained significant ( P =0.04 for trend). The alpha(2B)-AR genotype was not associated with intima-media thickness or carotid artery compliance. These findings indicate that subjects homozygous for the deletion allele of alpha(2B)-AR appear to have an increased risk of impaired endothelial function, which may provide an explanation for the previously observed increased risk of myocardial infarction in male subjects with this genotype. It is not known whether the association of the alpha(2B)-AR polymorphism with endothelial function is direct, or is mediated via altered sympathetic activation.
authors.affiliation: Department of Pharmacology and Clinical Pharmacology, University of Turku, Itäinen pitkäkatu 4B, Finland.
authors.forename: Paula
authors.initials: P
authors.lastname: Heinonen
authors.forename: Laura
authors.initials: L
authors.lastname: Jartti
authors.forename: Mikko J
authors.initials: MJ
authors.lastname: Järvisalo
authors.forename: Ullamari
authors.initials: U
authors.lastname: Pesonen
authors.forename: Jaakko A
authors.initials: JA
authors.lastname: Kaprio
authors.forename: Tapani
authors.initials: T
authors.lastname: Rönnemaa
authors.forename: Olli T
authors.initials: OT
authors.lastname: Raitakari
authors.forename: Mika
authors.initials: M
authors.lastname: Scheinin
mesh_terms: D000328:Adult; D000368:Aged; D001916:Brachial Artery / Q000000981:diagnostic imaging / Q000503:physiopathology*; D002318:Cardiovascular Diseases / Q000235:genetics*; D017536:Carotid Artery, Common / Q000000981:diagnostic imaging / Q000503:physiopathology; D004730:Endothelium, Vascular / Q000503:physiopathology; D017353:Gene Deletion; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011110:Polymorphism, Genetic; D018341:Receptors, Adrenergic, alpha-2 / Q000235:genetics*; D012307:Risk Factors; D014463:Ultrasonography; D014664:Vasodilation / Q000235:genetics*
chemical_list: D018341:Receptors, Adrenergic, alpha-2