Induction of endotoxin tolerance improves lung function after warm ischemia in dogs.
In shock models, induction of endotoxin tolerance (ET) is known to have a protective effect. The present study was designed to explore if ET is effective in protecting lungs from reperfusion injury. Twelve foxhounds were used as experimental animals. After a left thoracotomy, the left hilum was clamped for 3 h, followed by 8 h of reperfusion. In the treatment group (ET, n = 6), dogs were pretreated with incremental daily endotoxin doses of up to 60 microg/kg on day 6. The ischemia and reperfusion experiment was carried out on day 9. Control group animals (n = 6) were not subjected to endotoxin. After 8 h of observation, functional parameters of the reperfused lung of the ET and the control group were statistically different (P < 0.05) with respect to Po(2) [ET vs. control: 172.7 +/- 12.9 vs. 66.1 +/- 7.2 (SE) mmHg], compliance (16.0 +/- 1.2 vs. 8.3 +/- 1.0 ml/0.1 kPa), and the wet-to-dry ratio (9.4 +/- 0.8 vs. 16.7 +/- 1.2). After 3 h of warm ischemia and 8 h of reperfusion, pulmonary function and lung water content improved in the endotoxin-tolerant group.
Friedrich
Ivar
I
Thoracic and Cardiovascular Surgery, Institute of Epidemiology, Biometrics and Informatics, Martin-Luther-University, Ernst Grube Strasse 40, 06097 Halle, Germany. ivar.friedrich@medizin.uni-halle.de
Spillner
Jan
J
Lu
Er-Xiong
EX
Barnscheidt
Markus
M
Kuss
Oliver
O
Sablotzki
Armin
A
Schade
F Ulrich
FU
Borgermann
Jochen
J
D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D004285:Dogs; D004361:Drug Tolerance; D004731:Endotoxins / Q000494:pharmacology*; D006439:Hemodynamics; D006358:Hot Temperature; D007511:Ischemia / Q000503:physiopathology*; D008168:Lung / Q000378:metabolism / Q000503:physiopathology*; D010743:Phospholipids / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000378:metabolism; D011652:Pulmonary Circulation; D011659:Pulmonary Gas Exchange; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control
D004731:Endotoxins; D010743:Phospholipids; D011506:Proteins