[A thermostable Clostridium thermocellum lichenase-based reporter system for studying the gene expression regulation in prokaryotic and eukaryotic cells].
A new reporter system was developed to study the gene expression regulation in prokaryotic (Escherichia coli) and eukaryotic (Saccharomyces cerevisiae and mammalian) cells. The system was based on the modified bacterial lichenase gene (licBM2), which was shown to meet the requirements for a reporter. The gene product was active and did not undergo modification in heterologous hosts. Simple and sensitive methods were used to detect and to quantitate the lichenase activity. Inducible licBM2 expression was demonstrated with E. coli and yeast cells, allowing the system to be employed in dynamic studies.
Goldenkova
I V
IV
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia. irengold@vigg.ru
Musiĭchuk
K A
KA
Piruzian
E S
ES
D000818:Animals; D003013:Clostridium / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005057:Eukaryotic Cells / Q000502:physiology*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D016716:PC12 Cells; D010957:Plasmids / Q000235:genetics; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000235:genetics
D006026:Glycoside Hydrolases; C028020:licheninase