title: K(+)-induced changes in the properties of the hyperpolarization-activated cation current I(h) in rat CA1 pyramidal cells.
abstract: The effects of rises in external K(+) (K(ext)) on I(h) were investigated in CA1 pyramidal cells of rat hippocampal slices using the whole-cell patch clamp technique. At the basal K(ext) level (2.5 mM), hyperpolarization-activated cation current (I(h)) had a maximal amplitude of -350+/-60 pA which was enhanced by approximately 60 and approximately 95% at 5 and 7.5 mM K(ext), respectively. The midpoint activation voltage was significantly shifted from -80 mV in the negative direction to about -87 mV at both 5 and 7.5 mM K(ext), without appreciable alterations of the current kinetics. The maximal conductance was approximately 2.4 nS under control conditions and significantly increased to approximately 3.3 and approximately 5.6 nS at 5 and 7.5 mM K(ext), respectively. The reversal potential was shifted in the positive direction, from a control value of approximately -30 mV by approximately 6 and approximately 14 mV at 5 and 7.5 mM K(ext), respectively. Our data demonstrate that even moderate changes in K(ext) have a substantial effect on the properties of I(h).
authors.affiliation: Section of Clinical Neuropharmacology, Department of Neurology, Neurozentrum, Breisacher Strasse 64, 79106, Freiburg i.Br., Germany. surges@nz.ukl.uni-freiburg.de
authors.forename: Rainer
authors.initials: R
authors.lastname: Surges
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Freiman
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Feuerstein
mesh_terms: D000818:Animals; D004594:Electrophysiology; D005260:Female; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D007473:Ion Channels / Q000187:drug effects*; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000494:pharmacology*; D017966:Pyramidal Cells / Q000187:drug effects*; D051381:Rats
chemical_list: D007473:Ion Channels; D011188:Potassium