title: Isolation and characterization of cold-shock domain protein genes, Oryzias latipes Y-box protein 2 (OlaYP2) and Fugu rubripes Y-box protein 1 (FruYP1), in medakafish and pufferfish.
abstract: The Y-box protein (YP) family shares a nucleic acid binding domain, called cold-shock domain, that has been evolutionarily highly conserved from bacteria to human. The different YPs identified so far in vertebrates are thought to function as transcriptional activators, transcriptional repressors and/or translational repressors. Medakafish and pufferfish are very suitable vertebrate models for the study of developmental genetics and comparative genomics, respectively. Here we report the isolation of two teleost YP genes, medakafish Oryzias latipes (Ola)YP2 and Fugu rubripes (Fru)YP1, which are expressed in multiple tissues. Phylogenetic analysis demonstrated that OlaYP2 and FruYP1 belong to different subclasses of the cold-shock domain protein genes. Future studies in suitable model systems, like the medaka for developmental biology and Fugu for evolutionary genomics, are expected to contribute to our understanding of YPs.
authors.affiliation: Research School of Biological Sciences, Australian National University, Canberra, ACT 2601, Australia.
authors.forename: Enchshargal
authors.initials: E
authors.lastname: Zend-Ajusch
authors.forename: Ute
authors.initials: U
authors.lastname: Hornung
authors.forename: Carola
authors.initials: C
authors.lastname: Burgtorf
authors.forename: Götz
authors.initials: G
authors.lastname: Lütjens
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Shan
authors.forename: Manfred
authors.initials: M
authors.lastname: Schartl
authors.forename: Thomas
authors.initials: T
authors.lastname: Haaf
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015870:Gene Expression; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D009990:Oryzias / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D023741:Takifugu / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D014157:Transcription Factors