FCP1, a phosphatase specific for the heptapeptide repeat of the largest subunit of RNA polymerase II, stimulates transcription elongation.
FCP1, a phosphatase specific for the carboxy-terminal domain of RNA polymerase II (RNAP II), was found to stimulate transcript elongation by RNAP II in vitro and in vivo. This activity is independent of and distinct from the elongation-stimulatory activity associated with transcription factor IIF (TFIIF), and the elongation effects of TFIIF and FCP1 were found to be additive. Genetic experiments resulted in the isolation of several distinct fcp1 alleles. One of these alleles was found to suppress the slow-growth phenotype associated with either the reduction of intracellular nucleotide concentrations or the inhibition of other transcription elongation factors. Importantly, this allele of fcp1 was found to be lethal when combined individually with two mutations in the second-largest subunit of RNAP II, which had been shown previously to affect transcription elongation.
Mandal
Subhrangsu S
SS
Division of Nucleic Acids Enzymology, Department of Biochemistry, Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
Cho
Helen
H
Kim
Sungjoon
S
Cabane
Kettly
K
Reinberg
Danny
D
D000483:Alleles; D000818:Animals; D007611:Aprotinin; D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D005656:Fungal Proteins / Q000378:metabolism; D005838:Genotype; D006367:HeLa Cells; D006801:Humans; D007313:Insecta; D007700:Kinetics; D051379:Mice; D018345:Mice, Knockout; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000378:metabolism* / Q000502:physiology*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics; D019473:Transcription Factors, TFII / Q000378:metabolism; D014158:Transcription, Genetic; D035602:Transcriptional Elongation Factors
D005656:Fungal Proteins; D009687:Nuclear Proteins; D011994:Recombinant Proteins; C080991:SPT4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D035602:Transcriptional Elongation Factors; C090706:recombinant neutrophil elastase inhibitor R-020; D007611:Aprotinin; D010749:Phosphoprotein Phosphatases; C116022:carboxy-terminal domain phosphatase; C056604:transcription factor TFIIF