FGFR-related gene nou-darake restricts brain tissues to the head region of planarians.
The study of planarian regeneration may help us to understand how we can rebuild organs and tissues after injury, disease or ageing. The robust regenerative abilities of planarians are based upon a population of totipotent stem cells (neoblasts), and among the organs regenerated by these animals is a well-organized central nervous system. In recent years, methodologies such as whole-mount in situ hybridizations and double-stranded RNA have been extended to planarians with the aim of unravelling the molecular basis of their regenerative capacities. Here we report the identification and characterization of nou-darake (ndk), a gene encoding a fibroblast growth factor receptor (FGFR)-like molecule specifically expressed in the head region of the planarian Dugesia japonica. Loss of function of ndk by RNA interference results in the induction of ectopic brain tissues throughout the body. This ectopic brain formation was suppressed by inhibition of two planarian FGFR homologues (FGFR1 and FGFR2). Additionally, ndk inhibits FGF signalling in Xenopus embryos. The data suggest that ndk may modulate FGF signalling in stem cells to restrict brain tissues to the head region of planarians.
Cebrià
Francesc
F
Group for Evolutionary Regeneration Biology, Center for Developmental Biology RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Kobe 650-0047, Japan.
Kobayashi
Chiyoko
C
Umesono
Yoshihiko
Y
Nakazawa
Masumi
M
Mineta
Katsuhiko
K
Ikeo
Kazuho
K
Gojobori
Takashi
T
Itoh
Mari
M
Taira
Masanori
M
Sánchez Alvarado
Alejandro
A
Agata
Kiyokazu
K
D000595:Amino Acid Sequence; D000818:Animals; D002490:Central Nervous System / Q000196:embryology; D005346:Fibroblast Growth Factors / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010932:Planarians / Q000196:embryology / Q000235:genetics*; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor / Q000737:chemistry; D012038:Regeneration; D015398:Signal Transduction; D014981:Xenopus
D011506:Proteins; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors