Effect of diaspirin cross-linked haemoglobin (DCLHb) on mean arterial pressure during cardiopulmonary bypass in swine.
Diaspirin cross-linked haemoglobin (DCLHb) is a haemoglobin-based oxygen carrier which had been proposed as a resuscitative solution to replace red cell transfusion in many clinical situations. The present study was designed to evaluate the effect of different volumes of DCLHb 10% (1, 5 and 10 mL kg-1) on the cardiovascular system during cardiopulmonary bypass (CPB), and to determine the effect of DCLHb (18 mL kg-1) when added directly to the CPB prime in anaesthetized swine. DCLHb, when used as a priming solution, induced a significant increase (around 20%) in mean arterial pressure (MAP), which persisted during the entire period of CPB (P < 0.05) as compared with controls. Administration of increasing doses of DCLHb during the time course of CPB resulted in a progressive increase in MAP (P < 0.05), suggesting a linear dose-response relationship. Nicardipine, a calcium channel blocker, returned MAP to baseline. Finally, weaning of CPB was easier in animals that received DCLHb, thereby suggesting a potential protective effect of free haemoglobin in this particular clinical situation.
Ferrera
R
R
INSERM EMI-U 0226, Laboratoire de Physiologie, Lyon, France. ferrera@lyon151.inserm.fr
Hadour
G
G
Chiari
P
P
Montagna
P
P
Jegaden
O
O
Burhop
K
K
Lehot
J J
JJ
D000818:Animals; D001158:Arteries / Q000502:physiology; D001241:Aspirin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D001784:Blood Gas Analysis; D001794:Blood Pressure / Q000187:drug effects*; D001802:Blood Substitutes / Q000008:administration & dosage / Q000494:pharmacology*; D002315:Cardiopulmonary Bypass / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D006454:Hemoglobins / Q000008:administration & dosage / Q000494:pharmacology*; D009529:Nicardipine / Q000008:administration & dosage / Q000494:pharmacology; D019990:Perioperative Care; D013552:Swine
D001802:Blood Substitutes; D006454:Hemoglobins; D009529:Nicardipine; C083478:diaspirin-cross-linked hemoglobin; D001241:Aspirin