title: The DNA replication-related element-binding factor (DREF) is a transcriptional regulator of the Drosophila myb gene.
abstract: Drosophila melanogaster possesses a single gene, Dm myb, that is closely related to the vertebrate proto-oncogene c-Myb, and its other family members (A-Myb and B-Myb), all of which encode transcription factors. Dm myb is expressed in all proliferating cells throughout development, and previous studies demonstrate that Dm myb promotes both S-phase and M-phase in proliferating cells, while preserving diploidy by suppressing endoreduplication. We have initiated a characterization of the mechanisms that regulate Dm myb expression, and we report here that the transcriptional activator DREF (the DNA replication-related element binding factor) activates Dm myb transcription via two binding sites located in the 5' flanking region; that the Dm myb promoter lacks a prototypical TATA box sequence and instead appears to use an initiator/downstream promoter element (Inr/DPE) type promoter; and that Dm myb expression is regulated at the translational as well as transcriptional level.
authors.affiliation: Department of Molecular Genetics (M/C 669), University of Illinois at Chicago, College of Medicine, 900 South Ashland Avenue, Room 2368, Chicago, IL 60607-7170, USA.
authors.forename: Nikolai V
authors.initials: NV
authors.lastname: Sharkov
authors.forename: Gary
authors.initials: G
authors.lastname: Ramsay
authors.forename: Alisa L
authors.initials: AL
authors.lastname: Katzen
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D005786:Gene Expression Regulation; D020570:Genes, myb / Q000235:genetics*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D014176:Protein Biosynthesis; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016385:TATA Box / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D020121:5' Untranslated Regions; C099494:Dref protein, Drosophila; D029721:Drosophila Proteins; D020598:Proto-Oncogene Proteins c-myb; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D008156:Luciferases