Prevention of adhesions with crystalloids during laparoscopic surgery in mice.
OBJECTIVE
To evaluate the effect of saline and Ringer's lactate solutions in preventing adhesions during laparoscopic surgery in mice.


METHODS
Prospective, randomized trial (Canadian Task Force classification I).


METHODS
Academic research center.


METHODS
Ninety-two female Naval Medical Research Institute mice.


METHODS
Adhesions were induced laparoscopically by opposing bipolar lesions in the uterine horns and pelvic sidewalls, and saline or Ringer's lactate solution was added at different times during the procedure.


RESULTS
Adhesions were scored quantitatively and qualitatively for extent, type, and tenacity after 7 days under microscopic vision during laparotomy. After 45 minutes of pneumoperitoneum, neither solution reduced adhesion formation, but when added immediately after surgery they did (p = 0.002). Coagulation was not significantly different with addition of either solution immediately after coagulation. In the third experiment the presence of fluid during pneumoperitoneum decreased adhesion formation (p = 0.0001) but Ringer's lactate was more effective than saline (p = 0.0005).


CONCLUSIONS
Crystalloids reduced CO(2) pneumoperitoneum-enhanced adhesion formation in a laparoscopic mouse model, but Ringer's lactate solution was more effective than saline.
Elkelani
Osama Ali
OA
Centre for Surgical Technologies, Minderbroederstraat 17, 3000 Leuven, Belgium.
Molinas
Carlos Roger
CR
Mynbaev
Ospan
O
Koninckx
Philippe Robert
PR
D000818:Animals; D000077324:Crystalloid Solutions; D004195:Disease Models, Animal; D005260:Female; D005500:Follow-Up Studies; D007431:Intraoperative Complications / Q000517:prevention & control; D007552:Isotonic Solutions / Q000494:pharmacology*; D010535:Laparoscopy / Q000009:adverse effects* / Q000379:methods; D051379:Mice; D008815:Mice, Inbred Strains; D010952:Plasma Substitutes / Q000494:pharmacology*; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects; D011183:Postoperative Complications / Q000517:prevention & control; D011336:Probability; D011897:Random Allocation; D012016:Reference Values; D000077325:Ringer's Lactate; D012680:Sensitivity and Specificity; D012965:Sodium Chloride / Q000494:pharmacology*; D000267:Tissue Adhesions / Q000517:prevention & control*; D016896:Treatment Outcome
D000077324:Crystalloid Solutions; D007552:Isotonic Solutions; D010952:Plasma Substitutes; D000077325:Ringer's Lactate; D012965:Sodium Chloride