title: The hormonogenic tyrosine 5 of porcine thyroglobulin is sulfated.
abstract: Our previous results showed that sulfated tyrosines of thyroglobulin (Tg), the molecular support of thyroid hormonosynthesis, are involved in the hormonogenic process. Moreover, the consensus sequence required for tyrosine sulfation is present in most of the hormonogenic sites. These observations suggest that tyrosine sulfation might play a critical role in the hormonogenic process. In this paper we studied the putative sulfation of tyrosine 5 contained in the preferential hormonogenic site. Porcine thyrocytes were cultured with thyrotropin but without iodide to preserve the sulfation state of tyrosine 5 and then incubated or not with [35S]sulfate. Secreted Tg was purified and submitted to peptide sequence analysis which confirmed the known peptide sequence of the NH(2) extremity of Tg:NIFEYQV. The treatment of [35S]sulfate-labeled Tg by leucine aminopeptidase, which sequentially digested its amino-terminal extremity, released the same amino acids and further analysis by thin layer chromatography showed that the tyrosine was sulfated. We concluded that tyrosine 5 is sulfated but the role of sulfate group in the hormonogenic process remains to be elucidated.
authors.affiliation: INSERM U555, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard J. Moulin, FR-13385 Marseille Cédex 05, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Venot
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Nlend
authors.forename: David
authors.initials: D
authors.lastname: Cauvi
authors.forename: Odile
authors.initials: O
authors.lastname: Chabaud
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D013552:Swine; D013954:Thyroglobulin / Q000737:chemistry* / Q000378:metabolism; D013961:Thyroid Gland / Q000166:cytology; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis*
chemical_list: C005662:tyrosine O-sulfate; D014443:Tyrosine; D013954:Thyroglobulin; D007931:Leucyl Aminopeptidase