title: Excretion of herpes simplex virus type 2 glycoprotein D into the culture medium.
abstract: Glycoprotein D (gD) of herpes simplex virus type 2 (HSV-2) was excreted from infected cells into the medium. Peptide mapping analysis and lectin binding assays suggested that the gD in the medium is secreted after full glycosylation and cleavage at its C terminus. Release of HSV-2 gD was inhibited by addition of either tunicamycin or brefeldin A, suggesting that the gD in the medium was secreted through the endoplasmic reticulum and Golgi apparatus.
authors.affiliation: Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan1.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Murata
authors.affiliation: Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan1.
authors.forename: Fumi
authors.initials: F
authors.lastname: Goshima
authors.affiliation: Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan1.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Takakuwa
authors.affiliation: Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan1.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Nishiyama
mesh_terms: D001692:Biological Transport; D003208:Concanavalin A / Q000378:metabolism; D003470:Culture Media; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D018258:Herpesvirus 2, Human / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D037102:Lectins / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D003470:Culture Media; D006023:Glycoproteins; D037102:Lectins; D014764:Viral Proteins; D003208:Concanavalin A