[Modification of Chinese hamster ovary cells].
Chinese hamster ovary cells (CHO) are preferable to prokaryotic, yeast or insect cells as hosts for biopharmaceutical production due to the products are more similar to their natural conformation. However, CHO cells confront tremendous difficulties when cultured in large scale such as mal-adaptation to serum-free medium, apoptosis and over-growth without limitation. So in addition to optimizing CHO system in respect of medium, environment and expression vector, modification of CHO cells themselves has drawn more and more attention. Here the main progress in CHO-modification is reviewed.
Lai
Da-Zhi
DZ
Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. dzlai@yahoo.com
Qi
Lian-Quan
LQ
Yu
Chang-Ming
CM
Wang
Hai-Tao
HT
Chen
Wei
W
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D016466:CHO Cells / Q000187:drug effects / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D018797:Cell Cycle Proteins / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D005822:Genetic Vectors / Q000235:genetics; D014162:Transfection
D018797:Cell Cycle Proteins