[Expression of polyprotein of infectious bursal disease virus in Bombyx mori].
Segment A of the genome of infectious bursal disease virus(IBDV) encodes structure protein VP2 and VP3 and protease VP4. In this study a polyprotein gene of IBDV was inserted into a Bombyx mori baculovirus transfer vector pAcHLT--C and contransfected into BmN cells with linear genome DNA of virus Bm-BacPAK6. Dot hybridization suggested that the segment A of the virus genome was inserted in the genome of Bm-BacPAK6. The silkworm of fifth instars were infected by the recombinant virus and the immunogenicity of the infected larvae's blood were examined with ELISA, SDS-PAGE and Western blotting. It appears that the recombinant polyprotein has the property of immunoreactivity and the expression in larvae reached the pick 5-day post infection.
Lu
Mi-Jia
MJ
Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China.
Li
Jian-Rong
JR
Wang
Ying-Fei
YF
Jin
Yong-Feng
YF
Yu
Lian
L
D000818:Animals; D015153:Blotting, Western; D012831:Bombyx / Q000235:genetics* / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D007243:Infectious bursal disease virus / Q000235:genetics*; D020815:Polyproteins / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D020815:Polyproteins; D014764:Viral Proteins