title: Binding affinities and protein ligand complex geometries of nucleotides at the F(1) part of the mitochondrial ATP synthase obtained by ligand docking calculations.
abstract: F(0)F(1) ATP synthases utilize a transmembrane electrochemical potential difference to synthesize ATP from ADP and phosphate. In this work, the binding modes of ADP, ATP and ATP analogues to the catalytic sites of the F(1) part of the mitochondrial ATP synthase were investigated with ligand docking calculations. Binding geometries of ATP and ADP at the three catalytic sites agree with X-ray crystal data; their binding free energies suggest an assignment to the 'tight', 'open' and 'loose' states. The rates of multi-site hydrolysis for two fluorescent ATP derivatives were measured using a fluorescence assay. Reduced hydrolysis rates compared to ATP can be explained by the ligand docking calculations.
authors.affiliation: Institut für Physikalische Chemie der Albert-Ludwigs-Universität Freiburg, Albertstrasse 23a, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Steinbrecher
authors.forename: Oliver
authors.initials: O
authors.lastname: Hucke
authors.forename: Stefan
authors.initials: S
authors.lastname: Steigmiller
authors.forename: Michael
authors.initials: M
authors.lastname: Börsch
authors.forename: Andreas
authors.initials: A
authors.lastname: Labahn
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000465:Algorithms; D018360:Crystallography, X-Ray; D006868:Hydrolysis; D008024:Ligands; D008928:Mitochondria / Q000201:enzymology*; D008958:Models, Molecular; D011485:Protein Binding; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008024:Ligands; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases