title: Transcription profiling of Candida albicans cells undergoing the yeast-to-hyphal transition.
abstract: The ability of the pathogenic fungus Candida albicans to switch from a yeast to a hyphal morphology in response to external signals is implicated in its pathogenicity. We used glass DNA microarrays to investigate the transcription profiles of 6333 predicted ORFs in cells undergoing this transition and their responses to changes in temperature and culture medium. We have identified several genes whose transcriptional profiles are similar to those of known virulence factors that are modulated by the switch to hyphal growth caused by addition of serum and a 37 degrees C growth temperature. Time course analysis of this transition identified transcripts that are induced before germ tube initiation and shut off later in the developmental process. A strain deleted for the Efg1p and Cph1p transcription factors is defective in hyphae formation, and its response to serum and increased temperature is almost identical to the response of a wild-type strain grown at 37 degrees C in the absence of serum. Thus Efg1p and Cph1p are needed for the activation of the transcriptional program that is induced by the presence of serum.
authors.affiliation: Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada H4P 2R2. andre.nantel@bri.nrc.ca
authors.forename: André
authors.initials: A
authors.lastname: Nantel
authors.forename: Daniel
authors.initials: D
authors.lastname: Dignard
authors.forename: Catherine
authors.initials: C
authors.lastname: Bachewich
authors.forename: Doreen
authors.initials: D
authors.lastname: Harcus
authors.forename: Anne
authors.initials: A
authors.lastname: Marcil
authors.forename: Anne-Pascale
authors.initials: AP
authors.lastname: Bouin
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Sensen
authors.forename: Hervé
authors.initials: H
authors.lastname: Hogues
authors.forename: Marco
authors.initials: M
authors.lastname: van het Hoog
authors.forename: Paul
authors.initials: P
authors.lastname: Gordon
authors.forename: Tracey
authors.initials: T
authors.lastname: Rigby
authors.forename: François
authors.initials: F
authors.lastname: Benoit
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Tessier
authors.forename: David Y
authors.initials: DY
authors.lastname: Thomas
authors.forename: Malcolm
authors.initials: M
authors.lastname: Whiteway
mesh_terms: D002176:Candida albicans / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D010641:Phenotype; D013696:Temperature; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D003470:Culture Media; D014157:Transcription Factors