title: Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans.
abstract: Using inverse PCR, two new genes (btrN and btrS) were identified upstream of the putative glycosyltransferase gene btrM in the butirosin-biosynthetic btr gene cluster of Bacillus circulans. The upstream gene btrS showed significant homology with stsC of Streptomyces griseus, which encodes L-glutamine:scyllo-inosose aminotransferase in the biosynthesis of streptomycin. The function of BtrS was further confirmed by heterologous expression in Escherichia coli and chemical identification of the conversion of 2-deoxy-scyllo-inosose into 2-deoxy-scyllo-inosamine. The identification of BtrS as L-glutamine:2-deoxy-scyllo-inosose aminotransferase is the first report of the aminotransferase gene responsible for 2-deoxystreptamine biosynthesis.
authors.affiliation: Department of Chemistry, Tokyo Institute of Technology, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tamegai
authors.forename: Eriko
authors.initials: E
authors.lastname: Nango
authors.forename: Mieko
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Ota
authors.forename: Hisako
authors.initials: H
authors.lastname: Kuriki
authors.forename: Tadashi
authors.initials: T
authors.lastname: Eguchi
authors.forename: Katsumi
authors.initials: K
authors.lastname: Kakinuma
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002076:Butirosin Sulfate / Q000096:biosynthesis*; D005798:Genes, Bacterial; D005973:Glutamine / Q000378:metabolism*; D006595:Hexosamines / Q000096:biosynthesis; D007294:Inositol / Q000031:analogs & derivatives* / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D000637:Transaminases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D006595:Hexosamines; D005973:Glutamine; D007294:Inositol; D002076:Butirosin Sulfate; C075548:2-deoxyinosose; D000637:Transaminases; C007265:2-deoxystreptamine