Fractional isolation and physico-chemical characterization of hemicelluloses by a two-stage treatment from Haloxylon ammodendron and Elaeagnus angustifolia.
The cell wall material of Chinese shrubs Haloxylon ammodendron and Elaeagnus angustifolia was fractionated by successive extractions with ethanol/H(2)O (60:40, v/v) under acidic conditions (0.2 N HCl) at 70 degrees C for 4 h, and 2% H(2)O(2) at pH 11.5 for 16 h, respectively. The sequential two-step treatment resulted in the dissolution of 83.9% and 87.6% of the original hemicelluloses from dewaxed H. ammodendron and E. angustifolia, respectively. Xylose, glucose, and galactose were the major sugar constituents in the two acidic organosolv-soluble hemicellulosic preparations. The two alkaline peroxide-soluble hemicellulosic fractions were shown to be composed primarily of xylose, comprising over 80% of the total sugars. The results also showed that the two alkaline peroxide-soluble hemicellulosic fractions were more linear and acidic, and had higher molecular mass and thermal stability than the two acidic organosolv-soluble hemicellulosic preparations. The 2% H(2)O(2) posttreatment did not result in any significant changes in the macromolecular structure of the isolated hemicelluloses. It is probable that lignin protects hemicelluloses and cellulose from being attacked by peroxide.
Sun
Xiao-Feng
XF
College of Forestry, The North-Western Sciences and Technology University of Agriculture and Forestry, Yangling, People's Republic of China.
Sun
Run-Cang
RC
Lu
Qi
Q
Xu
Feng
F
Lin
Lu
L
D002473:Cell Wall / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods; D019663:Chenopodiaceae / Q000737:chemistry*; D031263:Elaeagnaceae / Q000737:chemistry*; D008031:Lignin / Q000737:chemistry; D008970:Molecular Weight; D010545:Peroxides; D010944:Plants / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*
D010545:Peroxides; D011134:Polysaccharides; C007916:hemicellulose; D008031:Lignin