Radiographic evaluation of the effect of endotoxin (LPS) plus calcium hydroxide on apical and periapical tissues of dogs.
The aim of this study was the radiographic evaluation of the apical and periapical region of dog teeth submitted to intracanal bacterial endotoxin (lipopolysaccharide, LPS), associated or not with calcium hydroxide. After removal of the pulp, 60 premolars were divided into four groups and were filled with bacterial endotoxin (group 1), bacterial endotoxin plus calcium hydroxide (group 2), saline solution (group 3), or periapical lesions were induced with no treatment (group 4), for a period of 30 days. Similar periapical lesions were observed in groups 1 and 4. The lamina dura was intact in groups 2 and 3. Bacterial endotoxin (LPS) caused radiographically visible periapical lesions, but when associated with calcium hydroxide, this endotoxin was detoxified.
Nelson-Filho
Paulo
P
Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Brazil.
Leonardo
Mario Roberto
MR
Silva
Léa Assed Bezerra
LA
Assed
Sada
S
D000818:Animals; D001485:Basement Membrane / Q000000981:diagnostic imaging; D001862:Bone Resorption / Q000000981:diagnostic imaging / Q000382:microbiology; D002126:Calcium Hydroxide / Q000627:therapeutic use*; D004285:Dogs; D004731:Endotoxins / Q000009:adverse effects* / Q000037:antagonists & inhibitors; D004926:Escherichia coli; D008070:Lipopolysaccharides / Q000009:adverse effects* / Q000037:antagonists & inhibitors; D010483:Periapical Diseases / Q000000981:diagnostic imaging / Q000382:microbiology; D010486:Periapical Tissue / Q000000981:diagnostic imaging* / Q000382:microbiology; D010513:Periodontal Ligament / Q000000981:diagnostic imaging; D011859:Radiography; D012387:Root Canal Filling Materials / Q000627:therapeutic use*; D012391:Root Resorption / Q000000981:diagnostic imaging / Q000382:microbiology; D018709:Statistics, Nonparametric; D019227:Tooth Apex / Q000000981:diagnostic imaging* / Q000382:microbiology
D004731:Endotoxins; D008070:Lipopolysaccharides; D012387:Root Canal Filling Materials; D002126:Calcium Hydroxide