title: Genotyping of Mycobacterium bovis by geographic location within Mexico.
abstract: The spacer oligonucleotide typing (spoligotyping) method was used to differentiate 62 Mycobacterium bovis isolates obtained from tissues with macroscopic lesions typical of tuberculosis in dairy cattle from different regions of Mexico. Our purpose was to see if a strain from one region was genetically different from those of other regions (with the long-term aim of doing molecular trace back of isolates obtained in the laboratory). Results from the genetic analysis indicate that M. bovis isolates cannot be grouped by geographic location due to a wide range of genetic types involved in dairy cattle infections. Isolates even from the same herd showed different spoligotypes but some isolates from different region had similar genetic patterns. Genetic typing without epidemiologic information does not seem to be a plausible method to trace back animals to source of origin to detect and eliminate sources of infection.
authors.affiliation: Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias-CENID-Microbiología, Km 15.5 Carretera Mexico-Toluca, Palo Alto, D.F. Delagación Cuajimalpa, C.P. 05110 Mexico. milian1@sunserver.uaq.mx
authors.forename: Feliciano
authors.initials: F
authors.lastname: Milián-Suazo
authors.forename: Victor
authors.initials: V
authors.lastname: Banda-Ruíz
authors.forename: Carolina
authors.initials: C
authors.lastname: Ramírez-Casillas
authors.forename: Camila
authors.initials: C
authors.lastname: Arriaga-Díaz
mesh_terms: D000818:Animals; D002417:Cattle; D003612:Dairying; D005838:Genotype; D005843:Geography; D008800:Mexico / Q000453:epidemiology; D009163:Mycobacterium bovis / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D014380:Tuberculosis, Bovine / Q000453:epidemiology* / Q000382:microbiology*