Anti-interleukin-8 autoantibodies in patients at risk for acute respiratory distress syndrome.
OBJECTIVE
To test the hypothesis that elevated concentrations of interleukin-8 associated with anti-interleukin-8 autoantibodies (anti-interleukin-8:interleukin-8 complexes) are found in patients at risk for acute respiratory distress syndrome who developed the disease.


METHODS
Measurement of anti-interleukin-8:interleukin-8 complex concentrations in previously collected bronchoalveolar lavage fluids. These fluids were obtained from patients at risk for acute respiratory distress syndrome who subsequently either recovered or developed acute respiratory distress syndrome.


METHODS
A unique population of patients at risk for acute respiratory distress syndrome was studied. There were 26 patients at risk for acute respiratory distress syndrome who were divided into three groups. Group I patients had high interleukin-8 concentrations and developed acute respiratory distress syndrome, group II had high interleukin-8 concentrations and did not develop acute respiratory distress syndrome, and group III had low interleukin-8 concentrations and did not develop acute respiratory distress syndrome. These patients were selected to test the hypothesis that presence of elevated concentrations of anti-interleukin-8:interleukin-8 complexes differentiates patients at risk for acute respiratory distress syndrome who developed acute respiratory distress syndrome from patients who did not.


RESULTS
Bronchoalveolar lavage fluid concentrations of interleukin-8 associated with the anti-interleukin-8 autoantibodies were significantly different between groups (p <.03). The amount of interleukin-8 bound to the anti-interleukin-8 autoantibody was higher in group I than in group II and group III.


CONCLUSIONS
Bronchoalveolar lavage fluid concentration of anti-interleukin-8:interleukin-8 complexes may serve as a marker of disease progression in patients at risk for acute respiratory distress syndrome.
Kurdowska
Anna
A
Department of Biohemistry, University of Texas Health Center, Tyler, USA.
Noble
James M
JM
Grant
Ian S
IS
Robertson
Colin R
CR
Haslett
Christopher
C
Donnelly
Seamas C
SC
D000936:Antigen-Antibody Complex / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis / Q000276:immunology*; D007416:Intestinal Perforation / Q000150:complications / Q000276:immunology; D009104:Multiple Trauma / Q000150:complications / Q000276:immunology; D010195:Pancreatitis / Q000150:complications / Q000276:immunology; D012128:Respiratory Distress Syndrome / Q000209:etiology / Q000276:immunology*; D012307:Risk Factors
D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D015415:Biomarkers; D016209:Interleukin-8