title: Genetic integrity of ex situ Genebank collections.
abstract: The genetic integrity of four accessions of the cross-pollinating species rye (Secale cereale L.) was investigated. Seeds available from the first and most recent regeneration cycles, multiplied 8, 12 (twice) or 14 times were fingerprinted using microsatellite markers. In all four accessions the allele numbers and frequencies changed after regeneration. Alleles present in the original seed sample were not detectable in the regenerated populations, whereas on the other hand, alleles were found in the recent seed sample, which were not observed in the investigated plants of the original one.
authors.affiliation: Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, D06466 Gatersleben, Germany.
authors.forename: Sabina
authors.initials: S
authors.lastname: Chebotar
authors.forename: Marion S
authors.initials: MS
authors.lastname: Röder
authors.forename: Victor
authors.initials: V
authors.lastname: Korzun
authors.forename: Andreas
authors.initials: A
authors.lastname: Börner
mesh_terms: D000483:Alleles; D018070:Biological Specimen Banks; D018556:Crops, Agricultural / Q000235:genetics / Q000592:standards*; D003433:Crosses, Genetic; D005787:Gene Frequency; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D009154:Mutation; D011058:Pollen; D012434:Secale / Q000235:genetics*; D012639:Seeds
chemical_list: D005819:Genetic Markers