NADPH-diaphorase and calcium binding proteins in the trigeminal nucleus oralis of rats.
We have examined the distribution of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) and the calcium binding proteins (CBPs), calbindin D-28k (CB), calretinin (CR) and parvalbumin (PV), in the trigeminal nucleus oralis (Sp5O). NADPH-d was detected by histochemistry while CBP was detected by immunohistochemistry. NADPH-d-positive neurons were distributed in the medial rostro-dorsomedial part (RDMsp5O) and dorsomedial part (DMsp5O) of Sp5O, and the rostrolateral part of the nucleus of the solitary tract (NTS). CB- and CR-positive neurons were mainly distributed in the dorsal part of Sp5O. In contrast, PV-positive neurons were mainly distributed in the ventral part of Sp5O. NADPH-d colocalized with CB (40%) and CR (20%) but not with PV in neurons of DMsp5O/ NTS. The mean cell sizes of neurons in RDMsp5O were larger than those in DMsp5O/NTS. PV-positive neurons were larger than NADPH-d-positive neurons. NADPH-d-, CB- and CR-positive neurons were generally small in RDMsp5O and DMsp5O/NTS. Few neurons were retrogradely labeled in RDMsp5O and DMsp5O from the thalamus, when numerous labeled neurons were in the principal and interpolar nuclei. These data indicate that NADPH-d histochemistry and CB, CR and PV immunohistochemistry identify a discrete cell population in Sp5O. Those labeled neurons in RDMsp5O and DMsp5O/NTS were considered to be involved in sensorimotor reflexive function of the intra-oral structures.
Munekawa
Noboru
N
Department of Oral Anatomy and Neurobiology, Osaka University Graduate School of Dentistry, Japan.
Sugiyo
Shinichi
S
Varathan
Vidya
V
Fukami
Hideyuki
H
Wakisaka
Satoshi
S
Shigenaga
Yoshio
Y
Takemura
Motohide
M
D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010320:Parvalbumins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017552:Solitary Nucleus / Q000378:metabolism; D013788:Thalamus / Q000378:metabolism; D014018:Tissue Distribution; D014278:Trigeminal Nuclei / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D010320:Parvalbumins; D009252:NADPH Dehydrogenase