Expansins in the bryophyte Physcomitrella patens.
Expansins are cell wall proteins which play a key function in basic processes of plant growth and differentiation. It has been proposed that expansins are likely to be present in all land plants and, to date, they have been reported in angiosperms, gymnosperms and pteridophytes. In this paper, we provide the first report and analysis of genes encoding expansin-like proteins in the bryophyte, Physcomitrella patens. Our analysis indicates that both alpha- and beta-expansins are present as gene families in this plant and expression analysis indicates that these genes are subject to a complex regulation by both hormonal and environmental factors. In particular, the expression of many expansin genes in P. patens is upregulated by stress conditions, suggesting that they play a role in the specific cellular differentiation displayed by P. patens in response to such stress. Finally, we provide the first report on the generation and analysis of a series of knockout mutants for individual expansin genes.
Schipper
Ori
O
Institute of Plant Sciences, Swiss Federal Institute of Technology, Zurich.
Schaefer
Didier
D
Reski
Ralf
R
Flemin
Andrew
A
D000040:Abscisic Acid / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000595:Amino Acid Sequence; D001593:Benzyl Compounds; D019068:Bryopsida / Q000166:cytology / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009154:Mutation; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D011687:Purines; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001593:Benzyl Compounds; D018076:DNA, Complementary; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011687:Purines; C095825:expansin protein, plant; C030737:indoleacetic acid; D000040:Abscisic Acid; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin