Routes of FA delivery to cardiac muscle: modulation of lipoprotein lipolysis alters uptake of TG-derived FA.
Long-chain fatty acids (FA) supply 70-80% of the energy needs for normal cardiac muscle. To determine the sources of FA that supply the heart, [(14)C]palmitate complexed to bovine serum albumin and [(3)H]triolein [triglyceride (TG)] incorporated into Intralipid were simultaneously injected into fasted male C57BL/6 mice. The ratio of TG to FA uptake was much greater for hearts than livers. Using double-labeled Intralipid with [(3)H]cholesteryl oleoyl ether (CE) and [(14)C]TG, we observed that hearts also internalize intact core lipid. Inhibition of lipoprotein lipase (LPL) with tetrahydrolipstatin or dissociation of LPL from the heart with heparin reduced cardiac uptake of TG by 82 and 64%, respectively (P < 0.01). Palmitate uptake by the heart was not changed by either treatment. Uptake of TG was 88% less in hearts from LPL knockout mice that were rescued via LPL expression in the liver. Our data suggest that the heart is especially effective in removal of circulating TG and core lipids and that this is due to LPL hydrolysis and not its bridging function.
Augustus
Ayanna S
AS
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
Kako
Yuko
Y
Yagyu
Hiroaki
H
Goldberg
Ira J
IJ
D000818:Animals; D002250:Carbon Radioisotopes; D015243:Cholesterol, VLDL / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000493:pharmacokinetics; D007783:Lactones / Q000494:pharmacology; D008066:Lipolysis / Q000502:physiology; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009206:Myocardium / Q000201:enzymology*; D000077403:Orlistat; D010168:Palmitates / Q000493:pharmacokinetics*; D012710:Serum Albumin, Bovine / Q000493:pharmacokinetics; D014280:Triglycerides / Q000493:pharmacokinetics*; D014316:Tritium
D002250:Carbon Radioisotopes; D015243:Cholesterol, VLDL; D004791:Enzyme Inhibitors; D005230:Fatty Acids, Nonesterified; D007783:Lactones; D010168:Palmitates; D014280:Triglycerides; D014316:Tritium; D012710:Serum Albumin, Bovine; D000077403:Orlistat; D008071:Lipoprotein Lipase