Gas-phase separations of protein and peptide ion fragments generated by collision-induced dissociation in an ion trap.
Ion mobility/time-of-flight mass spectrometry techniques have been used to examine distributions of fragment ions generated by collision-induced dissociation (CID) in a quadrupole ion trap. The mobility-based separation step prior to mass-to-charge (m/z) analysis reduces spectral congestion and provides information that complements m/z-based assignments of peaks. The approach is demonstrated by examining fragmentation patterns of insulin chain B (a 30-residue peptide), and ubiquitin (a protein containing 76 amino acids). Some fragments of ubiquitin show evidence for multiple stable conformations.
Badman
Ethan R
ER
Department of Chemistry, Indiana University, Bloomington 47405, USA.
Myung
S
S
Clemmer
David E
DE
D008401:Gas Chromatography-Mass Spectrometry; D007328:Insulin / Q000737:chemistry; D010084:Oxidation-Reduction; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D025801:Ubiquitin / Q000032:analysis
D007328:Insulin; D010455:Peptides; D011506:Proteins; D025801:Ubiquitin