title: Gene encoding a trehalose phosphorylase from Thermoanaerobacter brockii ATCC 35047.
abstract: A gene encoding a trehalose phosphorylase was cloned from Thermoanaerobacter brockii ATCC 35047. The gene encodes a polypeptide of 774 amino acid residues. The deduced amino acid sequence was homologous to bacterial maltose phosphorylases and a trehalose 6-phosphate phosphorylase catalyzing anomer-inverting reactions. On the other hand, no homology was found between the T. brockii enzyme and an anomer-retaining trehalose phosphorylase from Grifola frondosa.
authors.affiliation: Amase Institute, Hayashibara Biochemical Laboratories, Inc, Okayama, Japan. amaseken@hayashibara.co.jp
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Maruta
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Mukai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamashita
authors.forename: Michio
authors.initials: M
authors.lastname: Kubota
authors.forename: Hiroto
authors.initials: H
authors.lastname: Chaen
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Fukuda
authors.forename: Masashi
authors.initials: M
authors.lastname: Kurimoto
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D005964:Glucosyltransferases; C023181:alpha,alpha-trehalose phosphorylase