title: Protein splicing of the Deinococcus radiodurans strain R1 Snf2 intein.
abstract: Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein that splices by the Ala1 protein splicing pathway, with splicing dependent on adjacent residues.
authors.affiliation: New England Biolabs, Beverly, Massachusetts 01915, USA.
authors.forename: Maurice W
authors.initials: MW
authors.lastname: Southworth
authors.forename: Francine B
authors.initials: FB
authors.lastname: Perler
mesh_terms: D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D034301:Deinococcus / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D019154:Protein Splicing; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors