Identical T-cell receptor transcripts in multiple melanoma metastases.
Immune therapy for melanoma largely relies on preexisting T-cell responses. However, recent reports demonstrated the localized nature of such responses. Thus, we characterized the effect of immune therapy on the distribution of clonotypic T cells. To this end, we analyzed the T-cell receptor repertoire of multiple metastases of differentially treated melanoma patients revealing oligoclonal T-cell responses and the occurrence of identical T-cell clones in several metastases. However, these findings were not limited to immune therapy but were also observed after chemotherapy, suggesting its similar impact on the distribution of T cells.
Schrama
David
D
Department of Dermatology, Julius Maximilians-University, D-97080 Würzburg, Germany.
Fuchs
Eva
E
Bröcker
Eva-Bettina
EB
Thor Straten
Per
P
Becker
Jürgen C
JC
D002999:Clone Cells; D004586:Electrophoresis / Q000379:methods; D005260:Female; D006801:Humans; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008297:Male; D008545:Melanoma / Q000235:genetics / Q000276:immunology* / Q000556:secondary*; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology*
D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell